LOCUS       DAOSRG010000001        28607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_1814_length_28607_cov_9.864983, whole genome shotgun sequence.
ACCESSION   DAOSRG010000001 DAOSRG010000000
VERSION     DAOSRG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 28607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28607
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_28607_cov_9.864983"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..428
                     /locus_tag="PLM30_00005"
     CDS             135..428
                     /locus_tag="PLM30_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOR53582.1"
                     /translation="MAEKWKDRLTDQLCDAILSLETVDDVYRFLEDIATIGEIRALAQ
                     RLEVSKLLSEGFTYPQIAQQTGASTATISRVKKFLEYGADGYKLVLERLKKKG"
     gene            complement(459..1544)
                     /locus_tag="PLM30_00010"
     CDS             complement(459..1544)
                     /locus_tag="PLM30_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOR53583.1"
                     /translation="MREALQFCGSFPLNASSTLIKANLLSPSDPSRAVTTHPEILRAL
                     SMELFRAGCQDVMIGDNPGYIFRHQEEELFRKTGVAEICKDIPARFGLLSSRGFEEVR
                     PPKAQKLRSFRISSILMQSPQVIGVSKLKTHVETAMTGAIKNLFGAADTDTRKRAHAP
                     RTRTFLPEAIVDIFSARVPDLFVLDAVEGMEGFGPSHGRAKKVGWVAASTNGLALDYV
                     QSVMMGFSDPFSIPLLSIASKRLEGPGKREDIELKGARWEDLPCKGFERAPSIVSNVP
                     SLFRGAAHHVVTLKPYFNRDTCIRCGVCGKVCPVNAIQATPGGWPVIDRRRCVQCLCC
                     HEMCPTGAMEVRESLVSKLLRKIRRPH"
     gene            complement(1613..2281)
                     /locus_tag="PLM30_00015"
     CDS             complement(1613..2281)
                     /locus_tag="PLM30_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR53584.1"
                     /translation="MNLVKSCLDDLERIWKQGETVHGPYSDEPLDGLVLTILSQNTND
                     RNRDRAFAALKAHFNTWGEAAAASAQELSDVIRSGGLSNIKARRILEVLNRVRSHFGD
                     FSLKTLRGWETEDIRRFLEGTPGVGAKTIACVLLFDLGRPAFPVDTHIARVTRRLGWA
                     EASMAPEEIQILMEELVPPERYNEAHLNLITHGRRLCTSRKAFCVECPLRDRCPYPER
                     ESEG"
     gene            complement(2302..4863)
                     /locus_tag="PLM30_00020"
     CDS             complement(2302..4863)
                     /locus_tag="PLM30_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR53585.1"
                     /translation="MKNTDNLLTVEQLRKRTESGSLGFETTLDVPCLEDLIGQERAVQ
                     AMTFGMAIKNSGYNIFVVGDHGSGRTTYALERLQEQADSEPAPKDYIYAFNFDNPEEP
                     LAIDLPAGRARDLASHLEELVEELKSTLSKAFENSQYEDAKAQLVKEFQEQVNQIMEE
                     LRSWAKGKGFVIKRTPQGFVNIPMTLKGHDKDGEIEKADQPSGEGPLEGKSEADPSSP
                     GSQTDGKEEMREMVQEDFESLTEDQKKVLQGVSEEVSQRTLEILRRIRDLEKTLKEKI
                     KELESEISRAAIDPYLQETRDRFGKEGKIGKWLDALSEDIISNFNMFIAAARDEGAEV
                     DFSRYLANVFVSNDPSEGAPVVWETNPTYYNLCGKVEYESRQGMLTTDFRKIIPGVIH
                     KANGGYLVLQAEEVLRNFLSWDALKRALKTGELVIENLGEQLGVIPVSSLRPEAIPVD
                     LKVVMIGTRYLYHLLMIYDPEYQKLFKIKADFDIDMPRDAETERQMACFVAGFAKKEG
                     LLPFSSEAVSELIEWSARLSGNVDRMSTQFNRIAELIVESSAWAADAELDVVGAEQVR
                     RALREKNFRVNLVEEKIRRAFAEDSIRILTEGAVVGQINGLTVVDMGDHVFGHPVRIT
                     SNVYMGAEGIVNIEREVKMTGPIHNKGLLILESFLGRKFARVFPLSLTARITFEQTYG
                     GIEGDSASSTELYCILSALAEVPLKQGIAVTGSVDQHGNIQPIGGVNEKIEGFFEYCR
                     LRGFTGDQGVIIPRQNVKNLMLSHEVIDAVMSGEFKIWAVSTIEEGISILTGEVPGEM
                     DEKGEFTPRSIYSKVHSKLKDWYEKALKQKKHPGPGKKGKPEDRESTEVEDEAGK"
     gene            complement(4847..5902)
                     /gene="mnmA"
                     /locus_tag="PLM30_00025"
     CDS             complement(4847..5902)
                     /gene="mnmA"
                     /locus_tag="PLM30_00025"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR53586.1"
                     /translation="MKVVIGISGGIDSAVAAALLLDRGFEVVGVHLRIHSYPEVRKRI
                     ERIATALGIDCLSLEVGDLFRMKVLLPFFDDHRKGLTPNPCVICNEEVKFASLLSTAR
                     EVGAKKIATGHYARIFVDPGGPALGRCFDRDKDQSYMLYRLPPRSLGNILFPLGGMTK
                     GAVRELGERRFPGLFDGVAESNDICFIPRGELADAVERAAGPFPSGRVVALNGEVLGM
                     HRGLNRFTVGQRKGHSLSGGPWFVVEKRVNENELVVGRKCDLVVERVTCGDPVWHGEP
                     ASVLLLEGCTRYRGRSSPCIMEYADALGFACRFPEGVSGVAPGQSLVLYYNDRVFGGG
                     IIKTAKKGGPSGHEEHR"
     gene            complement(5910..8462)
                     /locus_tag="PLM30_00030"
     CDS             complement(5910..8462)
                     /locus_tag="PLM30_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR53587.1"
                     /translation="MDLAQAHSLDAYDIFKDLEVDPEKGLDLQRVEMLVKQHGPNQIN
                     PEEPAPWWKKLVVQFMTPMIYLLAGAALISLAMGEELDAAAIGVVILINGGIGFVTEF
                     RAEKALLALREMISPTAVVLREGVIRTINAASLVPGDIILLEAGDVVPADARIFEQYN
                     LGVDESALTGESFPVDKSGERLPVETVLADRKNCLFSGTAVVRGSGRAVIFATGMKTE
                     IGRISQLLSTVNKVETPLEERLARLGVLLIKMVVGIAILVTGLGIIQGRGIARMIETG
                     IALAVAAVPEGLPFVATMTLAIGVHRMARKNALVRNLSSVETLGSTTVICTDKTGTLT
                     MNDMVVGTVAPAHENCEGSLLRTSILCNKAHLVDDDCVGDPMEGALLRYASSRGVDIE
                     AVRKAAPILKEEPFDSRTMRMITWHSDGVAAKGAPERILELCRISCSPGGPVPLGIEM
                     KEEWTEKVEALAARGMRTLAFAWGEDEENLAFLGVSGIFDPPRPEVRDSVNSCINAGI
                     HVIMVTGDHLATARTIAGDVGILRQTGAKVLSGSALADMDESDLASKAREIAVIDRVI
                     PEHKLMIVRSLQRSGEVVAMTGDGVNDAVALKQADVGIAMGIQGTEVSKEASDIILQD
                     DRFSTIVAAIEEGRKIFDNIRKSVLYLLCCNLSEVVVVLAGIVLGLPSILLPLQILWI
                     NLVTDVAPALALALDPAEPDVMNRPPKKRQEDILSRWHMANIVFYGLIISLGVFTAYG
                     FSVRFHPGDTARATELCFHTLVFAQLFFVFNVRSSSLLRDPGKVLSNPWLLAGIGFSA
                     LLQFFIGHLPIMQVLLDIRPLSAVEWVTVISCALLPSLVAQVRKLFGKDMVR"
     gene            8580..10859
                     /gene="hypF"
                     /locus_tag="PLM30_00035"
     CDS             8580..10859
                     /gene="hypF"
                     /locus_tag="PLM30_00035"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOR53588.1"
                     /translation="MERHVRLQVSGVVQGVGFRPFCSRLSAEIGLRGSVRNTSAGVAI
                     ELFGDPEGIDSFIVRLQTDCPTLGHIQSIAMVLDERITEADVSSFEILESGSPGEGNA
                     LFPPDIATCPDCLRELRDPRNRRFRYPFTNCTNCGPRFTIIEGLPYDRPLTSMAGFAF
                     CERCRSEYNDPGDRRYHAQPVACEVCGPRLSLLSGGGSPLAEGDEALRECTRALAAGE
                     IVALKGLGGFHIACLPEDAPVLRLRERKKRPAKPFALMIRDDLVAEGLVTLSPYSRQL
                     LNSPRRPIVICPRKHLSGISPFVAPSQDSLGIMLPYTPLHHLIMEELPVLVMTSANLS
                     DEPLVSENGEALAKLKGVADLFLVHDRPITMKIDDSVVATAGKRSILLRRGRGYVPHP
                     VMTKREMPQILAAGAEMRSTFSLARGRTIYPSQYIGDLKQLDSAIYYEKALRHFLKLF
                     DLRPTLLAADLHPSFACTGIAKKVIGVPEDTLLVQHHHAHMAACLVENGFEGTALGII
                     LDGTGLGSDRHTWGGEFLLGDARTFTRLGHFQEAPLPGGDRAVLEPWRFALALMERAL
                     GQDAARSKAEKLWPERKHVFEKILSTLDAAPLTSSSGRLFDAVSAILGVRAEVSYEGQ
                     AAMELESAAKDYAEPAPFEIREEDGVLILDWKPLVEWLVAEGLHLGPGKASSAFHFGL
                     AEALSDVALELSRRTGVRETVLSGGVWQNKRLLSLALPLLERKGLRPLIHRVLSPNDE
                     CVSVGQAAVASLHWGIKQA"
     gene            complement(10922..11917)
                     /gene="glpX"
                     /locus_tag="PLM30_00040"
     CDS             complement(10922..11917)
                     /gene="glpX"
                     /locus_tag="PLM30_00040"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOR53589.1"
                     /translation="MNTPERNMALELVRATEAAAMAAGRWMGRGDRNASDNAAVNGMR
                     YMLNTIEMDGTIVIGEGEKDEAPMLFNGEKLGTGDPPEVDIAVDPIDGTRLLSLGRPN
                     AVSVVALSERNTLYDPKHIFYMNKIAVGPAAASAIDIEAPIQENLSRVAKALNKAVED
                     VTVVVLDRPRHEQLITEIREAGSRIRLISDGDIAGALMTCKAGSGIDLLVGVGGSPEA
                     VISACALKCVGGAMQCKLWPRNSEEAARCRELGMDLNAVLTIDDLVSSDNVFFAATGV
                     TDGEMLKGVRYEGDRIYSYSIVMRSKSGTIRYVDAIHSLKKLDLISSIDYGPRFE"
     gene            complement(11932..13257)
                     /gene="purB"
                     /locus_tag="PLM30_00045"
     CDS             complement(11932..13257)
                     /gene="purB"
                     /locus_tag="PLM30_00045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOR53590.1"
                     /translation="MIERYNTDEMESLWSQENRFRTWLEVELAVCEAWMKEGVIPEGS
                     LRNILERAEIDQERIKEIEATVHHDVIAFVTAVSEKIGPDGRYLHLGLTSSDVIDTAS
                     SLLMVRSLRLILDELELLSREILSKAFRYRHTPCMGRTHGVHAEPITFGFKLLNWYEE
                     LQRDRSRLLSAMEVVRVGKISGAVGTYAHCPPSVEERACDLLGLARAKVSTQILQRDR
                     HAQVVCALAILGSGLERIAQEVRHLQRTEVIEVLEPFYEGQKGSSAMPHKRNPVICER
                     ICGMARLLRGHNLASMENIPLWHERDISHSSVERVVWPDAFNLSHYMLKKAREVVEGM
                     TVLEDRMAKNLDLLKGLVFSQRVLLGLLEKGFSREDAYAIVQEAARISLEKEEPFLEV
                     LERDPRVAGSFKEQELRSLFDMGYYLRFIDDIFGRFPVGEAVTGDQDVT"
     gene            complement(13254..13775)
                     /locus_tag="PLM30_00050"
     CDS             complement(13254..13775)
                     /locus_tag="PLM30_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOR53591.1"
                     /translation="MQPAVVRIAGILHPDPGWLKWTVDLLTQQWGPVARISETFLFDH
                     TDYYKSISPVLFRTFLSFQGLAGAGELADWKIFSCSAEEKSGENRKVNIDPGYVNGSR
                     LVLASTKDHAHRIYLRDGIFAEVTLRFIKKKWVPFDCTFPDFSSGLYDDFLDGARSDW
                     LGLINSSGGSWVL"
     gene            complement(13781..14695)
                     /gene="ftsY"
                     /locus_tag="PLM30_00055"
     CDS             complement(13781..14695)
                     /gene="ftsY"
                     /locus_tag="PLM30_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOR53592.1"
                     /translation="MFSNLSERLGRVREKWGIQALFSERSRNGISWESLEEVFIAGDV
                     GVTMTERVIDALRAKVSVSGPDVDPAKLLSVVLRDMLLEVKGTGEPIRFSSAPTAVLL
                     VGVNGSGKTTTAAKLSWNLGREGRKTILAAADTFRAGAVEQLRIWGERAGARVVSHPS
                     KGDPGAVVFDAIKATEAKGCDCLIVDTAGRLHNRGNLMEEMARVSGIIDKNCAGWVRE
                     SFLVIDTVSGQNGLKQVEAFGKVVPLTGLVMTKYDHTAKGGVILSAGHELGVPVRYIG
                     LGEGMEDLVLFHPGEFVEALIADIRKEV"
     gene            complement(14717..15976)
                     /gene="ricT"
                     /locus_tag="PLM30_00060"
     CDS             complement(14717..15976)
                     /gene="ricT"
                     /locus_tag="PLM30_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOR53593.1"
                     /translation="MKSYLVIIGKPRYLGLLHSDDVDLDKGMEVVISSARGEEIGVVA
                     GPITMEQAETYRETLTNNGGDGAPRGGEPAFQDVKYLRPVTLQDERESASLREEEAQV
                     LAKGRELLKAHDLEMKLVDVEYLPDRKKLFFYFTAEQRVDFRAYVRDLAKEFKTRIEL
                     RQIGVRDESKVVKGISPCGMPCCCSYWLNRFAPICIRMVKEQNLALNPAKISGICGRL
                     MCCMGYEHEIYRELWKNLPSPGSKIKTPSVNYVVSGIDVAARRVRVTSPGTGELLIDV
                     DDFQDFRESVTRGEAWEKDKAIPAPLIEDLHEPGLSEEIREECDGVDKEPSDHPVDNV
                     REAEPRQGADSAERAGAFRGRRRKKRTTGPARVIEAHKPSSPTVPPAAAKPVNQNAPK
                     NARGGDKKRGRRRRPSGSRTADGGNGD"
     gene            complement(15948..16763)
                     /locus_tag="PLM30_00065"
     CDS             complement(15948..16763)
                     /locus_tag="PLM30_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53594.1"
                     /translation="MKNLGETMKGGKKEREAKNLENNLSSFVDEVSSVVGSDGARLPV
                     AGVMPFSLHMDFAMACARKILCFSGDGCGTCTACRAWSKTEQPDLLFFGAPGEPPGIK
                     DCRKLWEELSLKPVISRKRVAVIHSSHKLSLPAANSLLKISEETPSTGALIFLLEENV
                     LIPTLRSRLRLFQFRGSVGPEPGGEATPPGVHEFIKWVGKTRKLEPKVLAAEIQGWVG
                     FFLGKGDHCRAAGLDTIRLMAEKGKLTTPMAQDLVLVSLEGEVRLEELLGDNW"
     gene            complement(16760..17449)
                     /gene="tmk"
                     /locus_tag="PLM30_00070"
     CDS             complement(16760..17449)
                     /gene="tmk"
                     /locus_tag="PLM30_00070"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOR53595.1"
                     /translation="MTVSGHWGSSTSFDEGHPSLGLFITFEGIDGSGKSTLAGSVFRK
                     LAERLGMDRMVMTHEPGGWDGGEVLRETLIGGDLKSVLTETLLFLADRAEHVERVIRP
                     GLGAGKVILCERYSDSTLAYQVFGKSFPRGVVEEISAGGRFPRPDLTFWLDVPVRVAL
                     GRIVARKGKPDRFEKDGPLLERIRLGYGTLCHEEPARFKRMDGTKDPDELVDTMASSI
                     MALLMKKGSLR"
     gene            complement(17409..18680)
                     /locus_tag="PLM30_00075"
     CDS             complement(17409..18680)
                     /locus_tag="PLM30_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR53596.1"
                     /translation="MNREMRVGLVVFLAIVFLGALVFVSGGARFRQYGYSFGIVFPDA
                     MGLDTGAPVLVSGVESGKVVGIDLLEEGVLVRVNVRSHVIVPLDSRFIIDTGGLLGEP
                     RVKVTRGDSLENITEGDTVSGTIPPSLDEIMTDIKTSLGGLQSTFSNINTFLAKLSEA
                     AGEFQEFSKEARDQVKKAGDSLAGLTENIDSMVQENRGALMASLENLSQFLGNLKKVV
                     DQFDEGGTSGQDLRQMILRIKGAAESLEALSRKIEVALFDDTAPAPSRIQEVKEMVGK
                     ANRIISDIEDLSFGGKVGLYGGISGSGESDMMGDISLWVKSRSRKMGLLIGADDIGGD
                     PKVTAGLGLHGDRWEFWGGAVRGYPGAGFLLHPVGPQGPFSVIAQWWNENGGSWSLEG
                     RYRFDKSWGIFYRYLDKGDDSVGSLGFFYLF"
     gene            complement(18677..19447)
                     /locus_tag="PLM30_00080"
     CDS             complement(18677..19447)
                     /locus_tag="PLM30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR53597.1"
                     /translation="MDPVMRMQGVSVSFDGREVIGGMDMTLPVGEITCLMGPSGCGKT
                     TALKLASGLLKPDKGRIELLGKVIGPETQEDELVEVRKELGVVFQGGALFDSLSVGQN
                     IAFPLKYCREIEDPESISKMVLDMLEHVELEDVRDKYPAMLSGGMRKRVAIARSLVHS
                     PSLLLLDEPTTGLDPVTARHIDQLVVDLCGEFGFTALVVTHDIVSALGIGDTLLLMHE
                     GRIAWRGIPVEWERSDHPMVKSFAQGMVSVGRTGGEKM"
     gene            complement(19452..20231)
                     /locus_tag="PLM30_00085"
     CDS             complement(19452..20231)
                     /locus_tag="PLM30_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR53598.1"
                     /translation="MNLLSWIGRQAIELFSTLGFFFIVGLKAFTGIFSGTWSREDLFE
                     QLERVGVGSVLVVSITSAFTGMVLAVQTLDQFVRFGATGYIGGVIALSMVREMSPVLT
                     GLVVSGRIGAAMAAEIGSMKVTEQIDALRTFGLDPHVFVGVPRLLAGLVMLPVLTLFS
                     FVIGITGGFLYVAAHGVNAHLFRRSVEALLAPSDIYGGLLKAAVFGVLIVVSACGQGF
                     MAGQGARGVGTATTKAVVWGNMLILVFNYVLSSLLFGGIKG"
     gene            complement(20244..22025)
                     /locus_tag="PLM30_00090"
     CDS             complement(20244..22025)
                     /locus_tag="PLM30_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOR53599.1"
                     /translation="MFRKMIFLLVFPVCLCLVLPAYSSTFCVPGGTLSTASLSEGTKG
                     VAYTVIKGSEVVSFPVTVLSVMPSPKSPHNLILVRASGPVIEQTGGIAAGMSGSPVFV
                     GGKLVGAIGYGWSFSEHQMGLVTPIDEMAEIWNWADKVPSLKVPELKLHDPASGDLNL
                     FEPEPGNQKVSPPGDGGGLSAPLIGSGYSSRSARKIADALGNPVILLPGGGGDLSLPV
                     EYKPILRPGEAVSVLVAWGDVVLAATGTLTALSTDGRFLAFAHPFTNRGAVAFPLARS
                     WIHQVVPSLDTPFKIGTPTSIVGIVTQDRPQAIGGFIGRFAPVMDISLNFRDVDSGTE
                     TFKRFKTACDPFMMTKVIPEMITGLIDNVWGRVGEGSAKLTLKIEGGRLAEGWQRTNM
                     FFSSTDLTKILFSEFSDLVNAVSLNPFIEIAPFGVHIDIEATTEPRVVLIEDVNLSRK
                     TVPPGGSFDVEVTLRPFRKETVTHKMTLKVPMDAKGPCEVVVRGGGIEEPDQESIIMG
                     WRTIRSLDELLKEFSAMETNDEVVAEVRSIGLPGEDPLSPEEEESQKKLRSQVKEESI
                     KEGSFKSYRSNYFVDGLQRRMIKVIPE"
     gene            complement(22047..23151)
                     /gene="prfB"
                     /locus_tag="PLM30_00095"
     CDS             complement(join(22047..23078,23080..23151))
                     /gene="prfB"
                     /locus_tag="PLM30_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143330735.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOR53600.1"
                     /translation="MAQVPMTTVMEGLHSLVSELKESLDPEELSVEVEKLTKITSGEG
                     FWASENPQAVTRELSRLQGRLDAWKGVSREMEELQVMTELLGSGEDQELEGEFDRRAT
                     ALEGRIHDLQLLLLLDEEYDESNAILSIHSGAGGHDSQDWAEMLLRMYLRWAEEKGFQ
                     TRILEITEDEEAGIKSATVLVAGEYAYGYLKTERGVHRLVRISPFDTAKRRHTSFASL
                     NVSPELPDDAEIEIDPEDLRIDTYRSGGAGGQHVNMTDSAVRITHLPTGIVVSCQNER
                     SQHMNRHSAMVVLRGRLYERMLQERQEQINAIQGEKKEIGWGSQIRSYVLHPYTMVKD
                     HRTNLEKGNVQAVLDGDIDEFILSFLRFRKHHR"
     gene            23257..24252
                     /locus_tag="PLM30_00100"
     CDS             23257..24252
                     /locus_tag="PLM30_00100"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOR53601.1"
                     /translation="MRPFSKTKIAVLSELMGAGDLTVSGGALCLSLGLTRQAVWKAVN
                     ALREDGYVIESIPHKGYRFVSLPKNDLDPSLIEAFLLDCPWGHPVLYWESLNSTQVPA
                     KELARKGAPEGLIVTTSYQTSGRGRLGRRWVSAPEGGIFFSLVIRPALPPESIQLLSL
                     VSAMSVQDALFTLHGVRCQLKWPNDILWENSKLCGILTEVSSEPGLVHFAVTGIGINA
                     NSNNVSIDEGSETVSLSSITGKKIHRGELIAGVVRYLHGAVRGLEAPGGMKRALDEYS
                     SRCDTLGRKVRVVSDEGEVIGEAVALGEKGEIIVRTKDGTQTFTSADVNHLRALG"
     gene            complement(24257..25051)
                     /locus_tag="PLM30_00105"
     CDS             complement(24257..25051)
                     /locus_tag="PLM30_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53602.1"
                     /translation="MVLSLVILSSLCAGRIEALDIWVTDPWLAMLTRFIGGVQVTVHP
                     VITWDESGQPVKARTSPPAGGVVFSVMKGEGVPLLGKGLEEKYSTFYLFERSPCSSGL
                     DESFLDPATLPFIGLRTLQVLSISGPGDFEYFQRRLAEFQARLDSTVIVGKNMIGDKT
                     ILDLCWKYGRWLQAISGETVRPPADVKEKWSRSQALDVLETALGEAGRHGWVIVIDPW
                     TPATVRERLEGSKRVVELSPPRSEDDIILFLYDHCLKIWDHLRRNP"
     gene            complement(25129..25749)
                     /gene="pyrE"
                     /locus_tag="PLM30_00110"
     CDS             complement(25129..25749)
                     /gene="pyrE"
                     /locus_tag="PLM30_00110"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOR53603.1"
                     /translation="MARRDTSMESMEGIVRLLRASGALQEGHFALSSGLHSGHYLQCA
                     LFLMYPENAALAGSFLARKLSHLEPSLVVSPALGGIIIGHEVAKHLGVPFLFCERERG
                     EMKLRRFPAPGPARFVIIEDVVTSGRSVLEVKKVMDSVPETRFLAAGCIADRSDGLFI
                     EEKGLTSLVRFDFQNYNREECPLCREGIPLSEPGSRRLSSWDGGKV"
     gene            complement(25742..26461)
                     /gene="pyrF"
                     /locus_tag="PLM30_00115"
     CDS             complement(25742..26461)
                     /gene="pyrF"
                     /locus_tag="PLM30_00115"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOR53604.1"
                     /translation="MKKGGPGIILALDVRKPSEAERILETLGTHLRYVKIGPRLFTAG
                     GMPFVRRVIRAGFEVFLDLKLHDIPNTISEAVDLLARSGIWALTLHASGGEEMLKAAV
                     SAKGGARSEVLLLGVTVLTSIDQRGLDRSSPGSELRQSIKCRALLCKECGFDGIVCSP
                     RDLPLLDGLLPVGFLRVVPGIRDGQVSRDDQKRTAGIEEALDMGADFVVIGRPVIQSS
                     SLVGAMARFAERLDLWRKTNG"
     gene            complement(26489..27415)
                     /locus_tag="PLM30_00120"
     CDS             complement(26489..27415)
                     /locus_tag="PLM30_00120"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOR53605.1"
                     /translation="MSPFGTSLRTEVGGVDLDTPVIVASGVWPMDPALWPEGSLDGVG
                     AVCSKGLTLKPRDGNPGCRIVETYSGVLNSIGLQNSGVDHFVAQELPALSFAGRPIIV
                     NIAFETLDDLVEAVEVLKGSALKISALELNVSCPNVSRGGMTWGIHPDSLESAVTAAR
                     RAWDGILWVKLSPQAPSVGEFAELSQGCGAEAVVVGNTWLGTAIDNERERPFFENVFA
                     GLSGPAVFPLALRAVWEAAGAVEIPVIGCGGISRPQDALSMILAGASAVEVGTALFVD
                     SRLPASTCRELERHLRKKGLSSVGDLVGRARG"
     gene            complement(27396..28193)
                     /locus_tag="PLM30_00125"
     CDS             complement(27396..28193)
                     /locus_tag="PLM30_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOR53606.1"
                     /translation="MDSGMGKPGSPADRKGSLVFSRKVGENCRHIAVELSEATAPSAP
                     GQFFLVREWGVFDPLLGRPLASTASGDRRLEFLFQVTGRGTSRLASLPPGAALELRGP
                     CGNGFPAPVGEKLLLVAGTLGIAPFLGAVSALGEKVDVTAVLGVPGRGWEPFAEWCED
                     KIPGIRLNSDDGSLGRRGDPVEDSLTLVGPGRELWGCGPEAMLRELGRAYEKGCEKVL
                     VSLERRMGCGIGGCLSCSITTPSGMKRVCVDGPVFDWREVFGEPLRN"
     gene            complement(28210..>28607)
                     /locus_tag="PLM30_00130"
     CDS             complement(28210..>28607)
                     /locus_tag="PLM30_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR53607.1"
                     /translation="IATDHAPYHLDEKDLPFQEAAFGIASLECAVAAVLNEWARRGKP
                     CPLERILNLFTSGPASILPGKWKKLGKIEEGRPADLTVIDLDQERVVRVDEWESKARL
                     TPWDGKHLQGWPVMTLVGGRIVHSFNGSI"
BASE COUNT         5936 a         8834 c         7602 g         6235 t
ORIGIN      
        1 accccgcgag gcgaggccaa aattggggct ttcggggcag gtttcccggc gatttacttg
       61 cctttatgaa atacttgtcc aaaatggaaa gcagtgctag aatacgctaa agcatttatt
      121 aggagggatt ccgcatggca gaaaagtgga aggatcgtct gaccgatcaa ttatgcgatg
      181 caatcctgtc acttgagaca gtagacgatg tctaccgatt cctggaagat atcgccacga
      241 tcggggagat cagggctctc gcgcagagac tcgaggtttc caagctctta agtgaggggt
      301 ttacttaccc ccagatcgcc cagcagacag gagccagtac cgcgactatc agcagggtca
      361 aaaaattcct ggagtatggg gccgacggat ataagctcgt tctcgaacgc ctgaaaaaga
      421 agggctaact gcctggtccg ttttttccgg cgccctcctc agtgggggcg ccttattttc
      481 cttagcagtt tgctcactaa gctctccctg acctccatcg ccccggtggg gcacatctca
      541 tggcaacaga ggcattgaac acaccttcta cggtcgatca cgggccatcc cccgggggtg
      601 gcctggatcg cgttcaccgg gcaaaccttc ccgcagaccc cgcatctgat gcaggtgtcc
      661 ctgttgaagt atggtttcag agtgaccacg tggtgagccg cccctcgaaa aagggatggc
      721 acgttgctta cgatcgacgg cgctctctca aaccccttgc agggcaagtc ctcccaacgt
      781 gcccccttca gttcgatatc ttccctcttg ccgggaccct ccagcctctt cgaagcgatt
      841 gaaagcaggg gtatggaaaa ggggtcactg aaacccatca tcaccgactg gacataatcg
      901 agagccagac cgttggtcga agcggctacc cagccgactt tcttcgctct tccatgggag
      961 ggcccaaatc cctccattcc ctccaccgcg tcaagaacga aaagatcggg aaccctggcg
     1021 gaaaagatgt ccactatggc ttcagggagg aaggttcgtg ttctaggagc gtgggccctc
     1081 ttcctggtgt ccgtatcagc agcgccgaag aggttcttga tcgcaccggt catcgcggtt
     1141 tccacgtggg tcttgagctt tgacaccccg atgacttgag gggactgcat gagaatggac
     1201 gagatcctga aggatcgcag tttttgcgcc tttgggggcc ggacttcctc gaaacctcgg
     1261 ctggaaagga gtccaaatct tgcaggtatg tccttacaga tctccgccac gccggttttc
     1321 cggaatagtt cttcctcctg gtggcgaaaa atatatcccg ggttatcgcc gatcatgaca
     1381 tcttgacaac ccgccctgaa taattccatg gaaagggccc tcagtatctc agggtgcgtc
     1441 gtcaccgccc ttgacgggtc gcttggggaa agaaggttgg ccttgatgag ggttgaggag
     1501 gcattgagcg gaaaggaccc gcaaaactgc aaggcctccc tcaccgcctc ctgcaccgtt
     1561 gctctttcgt aagagtcgca ggggacgcag gagaccacaa agggaagccg tctcaaccct
     1621 ccgattccct ttcggggtag ggacaacggt cacgtaacgg gcactctacg cagaaggctt
     1681 ttcttgaagt gcaaagcctc ctgccatggg ttatcaggtt caggtgggct tcattgtagc
     1741 gctccggggg caccagttcc tccatcaaaa tctggatctc ctccggggcc atgctcgctt
     1801 ccgcccaacc gagccgtctc gtgaccctcg cgatgtgggt atccacggga aaggccggtc
     1861 gccccaggtc gaaaagcagt acacaggcga tcgtcttcgc ccccacacct ggggtgcctt
     1921 cgaggaacct tcggatatcc tccgtctccc atccccgcaa tgtcttcaaa gagaagtcac
     1981 cgaagtggga acggaccctg ttcaaaacct caaggatcct ccgtgccttt atgttggata
     2041 acccgccgga acggatgaca tcacttaact cctgggccga agcggcggcg gcctcccccc
     2101 aggtattaaa atgggccttc agagcagcaa aggccctgtc ccggttcctg tcattggtgt
     2161 tctgggagag gatcgtgaga acaagcccat caaggggttc atccgaatag ggcccatgga
     2221 cggtctcccc ctgcttccag atcctctcca ggtcgtcaag acacgactta acgaggttca
     2281 tcgagggttt tgtgaaaacc ctcatttccc ggcctcatcc tcgacttctg tactctcccg
     2341 atcctcgggt ttaccttttt tgcccggccc agggtgcttt ttctgcttca aggccttctc
     2401 gtaccagtct ttaagcttac tgtggacctt cgaatagatg ctccggggag tgaattcacc
     2461 cttttcgtcc atctcccccg gcacctcacc ggtgaggatc gagatgcctt cctcgatggt
     2521 cgagacggcc cagatcttga attctccgga cattacagcg tcgatgactt cgtgagaaag
     2581 catcaggttt ttgacattct gcctggggat gatgaccccc tggtcaccgg tgaatcctct
     2641 caaacggcag tattcgaaga aaccttcgat cttctcgtta acgccgccga tgggctggat
     2701 gttgccgtgc tgatcgacgg atccggtgac ggcaataccc tgctttaggg gcacttcggc
     2761 aagggcggag aggatacagt aaagttcagt ggaggaagcg ctatccccct cgatcccgcc
     2821 gtaggtctgc tcgaaggtga tcctggctgt aagggaaagc gggaaaaccc tggcgaactt
     2881 cctgccgagg aagctctcaa ggatcaaaag tcccttgttg tggatggggc ccgtcatctt
     2941 gacctccctt tcgatgttga cgatcccctc ggcacccata tatacattgg atgtgatcct
     3001 gacgggatgc ccgaaaacgt ggtcccccat gtccaccacg gtaaggccgt tgatctgtcc
     3061 caccaccgct ccttcggtga gtatcctgat ggagtcttcg gcgaaggctc tcctgatctt
     3121 ctcctcgacc aggttaaccc tgaagttctt ctccctcagg gctcttcgga cctgttcggc
     3181 cccgaccaca tccaattcgg cgtccgccgc ccatgccgag gattcgacga tgagctccgc
     3241 gatcctgttg aactgcgtgg acatccggtc aacgttgccc gatagcctgg ctgaccactc
     3301 gatcaactcc gacacggcct cggaagaaaa ggggagaagt ccttccttct tggcaaagcc
     3361 cgccacgaag caggccatct gtctttctgt ttccgcgtcc ctcggcatat ctatatcaaa
     3421 gtcggccttg atcttgaaaa gcttctggta ctcgggatcg tagatcatga ggagatggta
     3481 gaggtacctc gtcccgatca tgactacctt gagatccacg gggatcgcct cgggcctgag
     3541 cgaggagacg ggtataacgc ctaactgctc ccccaggttc tcgatcacca gctcccccgt
     3601 tttgagagcc ctcttgagtg catcccagga taggaaattc ctgagcactt cttccgcctg
     3661 aagcaccagg tagcccccgt tggccttatg tatgaccccg gggatgatct ttctgaaatc
     3721 ggtggtgagc atgccttgcc tgctttcata ctcgaccttg ccacagaggt tgtagtaagt
     3781 ggggttggtc tcccacacga cgggagcacc ctccgaggga tcgttggaca cgaaaacgtt
     3841 cgccaggtat cttgaaaaat cgacctcggc accctcgtcc ctcgccgctg cgatgaacat
     3901 attgaagttg gagataatat cctccgaaag ggcgtccagc cacttcccga tcttaccctc
     3961 cttgccgaac ctgtccctgg tttcctggag gtaggggtcg atcgcggcgc gggagatctc
     4021 ggactccaac tccttgatct tctccttcag cgttttctcc agatcacgga tcctcctgag
     4081 gatttccagg gtcctttggg atacttcctc ggatacaccc tgcaaaacct ttttctgatc
     4141 ctctgtaagc gattcgaaat cctcctggac catctccctc atctcttctt ttccatcggt
     4201 ctgagatccg ggggaggaag ggtcagcctc actcttacct tccagcggcc cttctccgga
     4261 gggttggtcg gccttttcaa tctcaccgtc cttatcgtgc cccttgaggg tcatcgggat
     4321 attaacgaag ccctgaggag tcctcttgat cacgaaacct tttcccttcg cccaggacct
     4381 caattcttcc atgatctggt tgacctgttc ctggaattcc ttcaccagtt gggccttggc
     4441 atcttcatac tggctgttct caaaggcctt gctcagcgtg cttttcagct cttcgaccag
     4501 ttcctccagg tgagaggcca ggtccctggc ccggcccgcg ggaaggtcaa tggcgagggg
     4561 ttcttccgga ttatcaaagt taaaggcata aatgtaatcc tttggggccg gttcggagtc
     4621 ggcctgctcc tgaagtctct caagggcgta cgttgttcgg ccgctgccat ggtcccccac
     4681 tacgaaaatg ttgtagccgc tgttttttat cgccattcca aaggtcattg cctggacggc
     4741 tctttcctgg cctatcaggt cttcaaggca gggaacgtcc agggtagtct cgaaacccag
     4801 tgaaccagat tctgtccttt tcctgagctg ttccaccgtc aaaagattat cggtgttctt
     4861 catggcctga tggcccccct ttcttcgccg tttttatgat accaccaccg aacactctgt
     4921 cattataata gagtacaagg gactgtcccg gtgccacgcc gctcacccct tcggggaaac
     4981 ggcaggcaaa acccagggca tcggcatact ccatgataca gggggaagac ctgccccgat
     5041 accttgtaca accctcgagg agcagcaccg aagcgggctc tccatgccag acgggatcgc
     5101 cgcaggtcac cctctccacg acaaggtcac actttcttcc aacaaccagt tcattctcat
     5161 ttactctttt ttcaaccacg aaccagggac ctccggagag ggaatgtcct ttcctctgcc
     5221 ctacggtgaa cctgttcaat cccctgtgca ttcccagtac ttccccgttt agagcgacca
     5281 ccctgccgga agggaaggga cccgcggcgc gctcgaccgc gtcggccagt tcgccccggg
     5341 ggatgaagca gatgtcgttg ctctcggcta ctccgtcgaa tagtccggga aatcgtctct
     5401 cacccagttc gcggacggct cccttggtca tcccccccag agggaacagt atgttcccaa
     5461 gggatcgcgg cggtaacctg tagagcatgt aactctggtc cttgtcgcgg tcaaaacacc
     5521 tgccgagagc ggggccgccg ggatccacaa atatcctggc gtagtggcct gtcgcgatct
     5581 ttttggcgcc aacctctctt gccgtggata ggagtgaggc aaacttgacc tcctcgttgc
     5641 agatcacgca tgggttggga gtaagcccct tccgatgatc atcgaagaag ggcaggagaa
     5701 ccttcatcct gaaaagatcc cccacttcca gcgacaagca gtctatccca agcgcggtgg
     5761 ctatcctctc tatccgcttt ctcacctcag ggtagctgtg gatccgaaga tgaacaccga
     5821 cgacttcaaa acccctatcg aggaggagag ccgcggcgac cgcgctgtcg atccccccgc
     5881 tgatcccaat cactactttc atagcccgat cacctgacca tatccttacc gaacaacttt
     5941 ctcacctggg cgacaaggga gggaagaagg gcacaggaga tcaccgtaac ccattcaacg
     6001 gccgacaggg gcctgatatc caataggacc tgcatgatgg gaagatggcc gatgaaaaac
     6061 tggagaagag cggaaaaacc gatccccgcc aggagccagg gatttgacag gactttccca
     6121 gggtcccgca gcaaggaact gctcctcacg ttaaacacaa agaaaagctg cgcgaacaca
     6181 agggtatgga agcataattc cgtcgcccgg gcggtatctc cagggtggaa tcggaccgaa
     6241 aatccatagg cggtgaaaac acccagcgaa atgatcaaac catagaaaac tatgttcgcc
     6301 atgtgccacc gggagaggat atcctcctgc ctcttcttgg gcggcctatt catcacgtcg
     6361 ggttcggccg ggtccagggc aagagcaagg gcaggcgcca cgtcggtgac gaggttgatc
     6421 cataatatct ggagcggaag gaggatcgat ggaaggccca ggacaatgcc cgcgaggacc
     6481 acgactacct cgctgagatt gcagcacaac aggtaaagca ccgatttcct gatattgtca
     6541 aagatcttcc ttccttcctc gatggctgcc acgatcgttg aaaaacggtc gtcctgaagg
     6601 ataatgtcgg aagcctcctt gctcacctcg gtcccctgta tacccatcgc gatccctacg
     6661 tcagcctgct tgagggccac cgcgtcgtta accccgtcgc cagtcatggc tacgacctcg
     6721 ccggaccgct gtagcgatct cactatcatg agcttgtgtt ccgggatgac cctatcgatg
     6781 acggcgatct ctctcgcctt ggaggcaaga tcgctctcat ccatatcggc cagggctgaa
     6841 ccggagagga ccttagcccc cgtctggcgg agtatgccca cgtccccggc aatggtccgg
     6901 gccgtcgcca ggtggtcacc ggtcaccatg atgacatgga tacccgcatt gatgcaggaa
     6961 ttcaccgaat ccctcacttc gggccgagga gggtcgaaaa tgcccgaaac cccaagaaag
     7021 gcgaggttct cctcgtcctc tccccaggcg aaagccaggg tccgcatacc ccgggccgcc
     7081 agggcttcga ctttttctgt ccattcctct ttcatttcga tccctagcgg gactggtccg
     7141 cctggggaac aggagatcct gcatagttcc aggatccttt cgggcgcccc cttcgctgca
     7201 acaccatcgc tgtgccaggt gatcatcctc atggtcctgg agtcgaaggg ttcttccttc
     7261 aggatcggtg cggccttcct cacggcttcg atatccaccc cccgggaaga cgcgtatcgc
     7321 aggagagctc cttccatggg gtcacccacg cagtcatcat cgacgaggtg tgccttgttg
     7381 cataggatgg aagtccgcag gagcgacccc tcacagtttt catgggcggg ggccacggtc
     7441 cccaccacca tgtcgttcat cgtaagggtc cccgtcttgt cggtgcagat caccgtcgtg
     7501 gagcccaagg tctccacgga ggacaggttt ctcacaaggg cgttcttcct ggccattcgg
     7561 tggaccccaa tggccagggt catggtggca acaaagggta acccctcggg tacggccgcc
     7621 acggccaggg cgatgccagt ctcgatcatt cgggcgatcc ccctgccctg gatgatcccc
     7681 agtcccgtaa ccaagatcgc aatgccgacg accatcttga tcagtaaaac cccaagcctg
     7741 gccaagcgtt cctcaagggg ggtctccacc ttgttcactg tcgagagaag ctggctgatc
     7801 ctgcctatct ccgtcttcat ccccgttgcg aagatgacag cccttcccga tcccctcacg
     7861 acggcggtcc ccgaaaaaag gcagttcttc ctgtcggcca gtacggtttc aacgggtagc
     7921 ctttccccgc ttttatcaac ggggaaggat tcccctgtca gggccgattc gtcgactcca
     7981 aggttgtact gctcgaaaat gcgtgcatcg gcggggacca cgtcacccgc ctcgagcagg
     8041 attatatcgc ccggtacgag gctggccgcg ttgatggtcc tgatcacccc ttcccggaga
     8101 acgacagcgg tcggtgatat catttcccgc agtgccaaaa gtgctttttc cgcccggaat
     8161 tcggtaacga aaccgatgcc gccgttgatc aggatgacta cgcctatcgc tgcggcgtcg
     8221 agttcctctc ccatggcgag gctgatcagg gccgctcccg ccagaagata gatcatgggt
     8281 gtcatgaact gcacgaccag ctttttccac cagggagccg gttcctcagg gttgatctgg
     8341 tttggtccat gctgtttcac cagcatttcc accctttgaa ggtcaaggcc cttttcgggg
     8401 tcgacctcga gatccttgaa gatgtcgtag gcatcaagag aatgggcttg ggctaggtcc
     8461 atatttttcg ttgctccctt cgagttagac gaatcgtacc ccccggccgc ctcatgaaaa
     8521 agaaagggct tgagcctccg agagttgggc attacaattc ccggagaccg gagggagaaa
     8581 tggaaagaca cgtaaggctt caggtttccg gggtagtgca gggtgttggg ttccgtccct
     8641 tctgcagcag gctttcagcc gagatcggtc tcaggggttc ggtgaggaac acttccgccg
     8701 gcgtcgcaat agagctcttc ggcgaccctg aaggcatcga ttccttcatc gtgcggctgc
     8761 agaccgattg tcccaccctg gggcatatcc agtcaatcgc gatggtcctt gacgagagga
     8821 tcaccgaggc cgatgtgagc tccttcgaaa tcctggaaag cggatccccg ggagagggga
     8881 atgccctgtt cccccccgat atcgccactt gccccgattg cctcagggag ttgcgcgatc
     8941 cgcggaacag gaggttccga taccctttca cgaactgcac caattgcggc ccccggttca
     9001 ccatcattga ggggctcccc tatgaccgac cccttacatc catggccggg ttcgcttttt
     9061 gtgaaagatg ccggtcggaa tacaatgacc ccggcgaccg ccggtatcat gcccagccgg
     9121 tggcctgtga ggtttgcggg cctcggcttt ctctcctgtc cggtggcggc tccccgctcg
     9181 cggaaggcga cgaggccctg cgcgaatgca cccgggcctt ggccgccggc gagatcgtcg
     9241 ccttgaaggg ccttggaggt ttccatatcg cctgtcttcc tgaagacgcc cccgtgctcc
     9301 ggttaaggga gaggaaaaaa agaccggcca aacccttcgc attgatgatc cgcgatgacc
     9361 ttgtcgccga agggcttgtg actctgtccc cgtactccag gcaacttttg aattccccaa
     9421 ggcgacccat cgtcatctgt ccccgcaaac atctctcggg gatatcccct tttgtcgccc
     9481 cctcccagga ttccctgggc atcatgttgc cctatacgcc gctgcatcac ctgataatgg
     9541 aagaactccc cgtcctggtc atgaccagcg ccaacctgag cgacgagccc ctggtctcag
     9601 aaaacggaga agccctcgcg aagctgaagg gtgtggcgga ccttttcctg gtccatgacc
     9661 gacccataac catgaagatc gacgactcgg tggtcgccac ggcgggcaag aggtccatac
     9721 tcctccgcag gggacggggg tatgtgcccc acccggtcat gacaaaacgg gagatgcccc
     9781 agatcctggc tgccggtgcc gagatgagat ccaccttttc actggccagg gggaggacga
     9841 tataccccag ccaatacatc ggggatctga agcagctcga ttccgcaata tattatgaga
     9901 aggccctgag gcatttttta aagctcttcg acctaagacc gaccctcttg gcggcagacc
     9961 tccacccttc ctttgcctgc acgggcatag ccaagaaagt gatcggggta ccggaggata
    10021 ctctccttgt acagcatcat catgcccaca tggcggcatg cctggtcgag aacgggttcg
    10081 agggcacggc cctgggtatc atccttgatg ggaccggcct gggaagcgac aggcacacct
    10141 gggggggtga atttctcctc ggtgatgccc ggaccttcac ccggctgggg catttccaag
    10201 aggcacctct ccctggaggg gacagggccg tgctggaacc ttggcgattc gccctcgcgt
    10261 tgatggaaag ggcccttggc caggatgccg ccaggagtaa ggcggaaaag ctttggcccg
    10321 agcgaaaaca tgtctttgaa aagatcctgt ccaccctcga cgccgctccc cttacatcat
    10381 cctccggcag gcttttcgat gccgtatcgg ccatattggg agtaagagcg gaagtctcct
    10441 acgagggcca ggccgcgatg gaactggagt ccgccgcaaa ggattacgcc gagcctgctc
    10501 ccttcgaaat aagggaggag gacggggtgc tcatactgga ttggaagccc ctggtggaat
    10561 ggctcgttgc ggagggtctt cacctgggtc cgggcaaggc ttcatcggcg ttccacttcg
    10621 gtctcgccga ggccctttct gatgttgctc tcgaactttc aaggaggacc ggggtcaggg
    10681 aaacggtcct ctccggtggc gtctggcaga ataaaaggct tctttccctc gcccttccac
    10741 tactggaaag gaaaggacta aggcccctta tccaccgtgt cctttcgccc aacgacgaat
    10801 gcgtatccgt cggccaggcg gcggtggcat cgctgcactg gggcataaaa caggcatagg
    10861 tggcggataa tcccgagcct tcgctaaaag ggacgatccc gctttcggat gggctgacct
    10921 tctattcgaa ccgtggaccg taatctatgc tgcttatgag gtccagcttt tttaggctgt
    10981 gtatggcgtc tacgtacctt atggtcccgc tcttcgagcg catcacgatt gagtaggaat
    11041 agatcctgtc cccctcgtac ctcacgccct ttagcatctc cccgtcggtc acgccggtgg
    11101 ccgcgaaaaa cacgttatcc gaggatacca ggtcatcgat cgtaaggacg gcgttcaagt
    11161 ccatccccaa ttccctgcac cgggcggcct cctcggagtt cctgggccaa agcttgcact
    11221 gcatggcccc gcccacgcac ttcagggcac aggcgcttat taccgcttcc ggcgaccccc
    11281 ccacgcccac caggaggtcg ataccgctcc ctgccttgca cgtcatgagc gcgccggcga
    11341 tatccccgtc cgagatcagc cttatccggg accccgcttc cctgatctcg gtgatcaact
    11401 gttcgtgcct tggtctgtcg agtacgacga cggtgacatc ctcgacggcc ttgttcaaag
    11461 ccttggccac tctggagagg ttttcctgga tcggggcttc gatatcgatc gcggaggcgg
    11521 cggcaggccc cacggctatc ttgttcatgt aaaaaatatg tttcggatcg taaagggtgt
    11581 tccgttctga aagggccacc acgctcacgg cgttgggcct tcccaaggag agaagcctcg
    11641 tcccatcgat ggggtccacg gcgatatcca cctctggggg atcgcccgtg ccgagcttct
    11701 cgccgttaaa gagcatgggc gcttcgtcct tctcgccttc gccgatcaca atggtaccgt
    11761 ccatctctat ggtgtttagc atgtagcgca tgccattgac cgcggcattg tccgaggcgt
    11821 tgcggtcacc ccggcccatc cagcgaccgg ccgccatggc ggccgcttcg gtcgctctca
    11881 ccagttccag ggccatattc ctttcgggtg tgttcattta aagccctcct ttcaagtcac
    11941 gtcctggtct cccgtaacgg cttctccaac cgggaacctt ccgaaaatat cgtcaatgaa
    12001 tcttaaataa taacccatgt cgaacaagga tctcaactcc tgctccttga aggatcccgc
    12061 gactctcggg tccctctcga gtacctccag gaaaggttct tccttttcca ggctgatcct
    12121 ggcggcttcc tgaacgatag cataggcatc ctcacgggaa aagcccttct ccagaagccc
    12181 cagcagaact ctctggctaa acaccagccc cttgagcagg tccaggtttt tcgccatcct
    12241 gtcttcaagg accgtcatgc cctccacgac ctcccgggcc ttcttgagca tgtaatggga
    12301 caggttgaaa gcgtcaggcc acaccaccct ctccaccgaa gaatggctta tatccctttc
    12361 atgccagagc gggatgtttt ccattgaggc caggttatgg cctctaagca gcctcgccat
    12421 cccgcagatc ctctcgcata tcacgggatt cctcttgtgg ggcatcgcgg aggaaccctt
    12481 ctgtccctcg taaaagggtt cgaggacctc gatgacctcc gtcctctgca aatgccttac
    12541 ttcctgggcg atcctttcca gccccgaccc gaggatggcc agggcgcaga ccacctgggc
    12601 gtggcggtcc cgttgaagga tctgagtgga tactttggcg cgggccagcc ccaggagatc
    12661 gcaggctctt tcctcaaccg acggtgggca gtgggcatag gtgcccaccg ccccggagat
    12721 cttgcccacc ctcaccactt ccatggcaga aagcagccgg ctccggtccc gttggagctc
    12781 ctcgtaccag tttaggagtt tgaaaccgaa ggtgatcggc tcggcatgga ccccgtgggt
    12841 cctccccatg cacggggtat gcctgtatct gaaagctttc gagaggatct cccgggagag
    12901 tagttccaat tcgtcgagga tcaaccttaa cgaccttacc atgaggagcg acgaggccgt
    12961 atcgatcaca tcgctgctgg tcaagcccag gtgaaggtac ctcccgtcgg gcccgatctt
    13021 ctccgaaaca gcggtgacaa aggctatcac gtcatgatgc acggtggcct cgatctcctt
    13081 gatcctttcc tggtcgatct cagccctttc gagaatattc ctgagcgatc cctcggggat
    13141 gaccccttct ttcatccagg cttcgcaaac ggccaattcg acctcaagcc aggtcctgaa
    13201 gcggttctcc tgcgaccaaa gactctccat ttcatcggtg ttgtatcttt cgatcaaaga
    13261 acccaactcc ccccggatga attgatcaag cccagccaat ccgatctggc tccatcgagg
    13321 aaatcatcgt acaagccgct ggaaaaatca gggaaggtgc agtcgaaggg gacccacttt
    13381 tttttaataa aacgcaatgt cacctcggcg aagatcccgt cccgaaggta tatcctgtgg
    13441 gcgtggtctt tcgtcgacgc caggacaagc cgtgaaccgt taacataacc cgggtcgatg
    13501 ttgaccttcc ggttttcgcc gctcttttcc tccgccgaac aggaaaagat cttccaatcc
    13561 gccagctccc ccgcgcccgc tagcccctgg aaggatagaa aagtcctgaa gaggacgggg
    13621 gaaatgcttt tgtagtaatc cgtatgatcg aacagaaagg tttcgctgat cctagccacg
    13681 ggaccccatt gttgggtaag aaggtcaacg gtccatttga gccagccagg atcgggatga
    13741 aggatgcccg ctatcctgac cacggcgggt tgcatcgata tcacacctct tttcggatat
    13801 cggcaatgag agcttccacg aattcgcccg gatggaagag gaccaggtct tccatcccct
    13861 ctcccagccc tatgtaacgg acaggaacgc ctaactcatg accggcggaa aggatcaccc
    13921 cgcccttggc ggtatggtca tacttggtca tcaccaggcc cgttagcggg actacttttc
    13981 cgaaggcttc gacctgttta aggccgtttt gcccggaaac ggtgtctatc acgagaaagg
    14041 attcccttac ccacccagcg caattcttat cgatgatgcc cgagaccctg gccatctctt
    14101 ccatcaggtt gccccggtta tgaagcctgc cggcggtatc cacgatcagg cagtcacagc
    14161 ccttggcctc cgttgccttg atggcgtcaa agaccactgc tccggggtcg ccctttgagg
    14221 ggtgagacac caccctcgca ccggctcttt ctccccagat ccggagctgt tcgacggccc
    14281 cggcacggaa tgtgtcggcc gcggcaagga tcgttttcct gccttcccgc ccaaggttcc
    14341 aggagagttt cgcggcggtg gtcgtcttgc cgctcccgtt gaccccgacg agcaggaccg
    14401 ccgtcggtgc cgatgaaaaa cggatcggct cacccgttcc ctttacctcc aggagcatat
    14461 cgcgaagtac gacggacaat aacttcgctg gatcgacatc ggggccgctg accgaaacct
    14521 ttgcacgcag ggcgtcgatc accctttccg tcatggtcac gccgacatcg cccgcgatga
    14581 agacctcctc cagggattcc caggagatcc cgttccttga acgctcggaa aagagcgcct
    14641 ggatacccca tttttctctt accctgccga gacgctcgct cagattgctg aacaaggccg
    14701 tttcacctcc gcagggttaa tcgccgttac cgccgtcagc ggtcctcgaa cccgagggtc
    14761 tgcggcgccg accccgcttc ttgtcaccgc ccctggcatt ctttggcgcg ttctggttca
    14821 ccggcttggc cgcggctggg ggtactgttg gacttgaggg cttatgggcc tcgatgaccc
    14881 tggcgggtcc cgtcgttctc ttttttcttc gccgccctcg aaaggcccct gccctctcgg
    14941 cggagtcggc gccttgtcgc ggttctgcct cgcgaacgtt atcaacgggg tgatccgaag
    15001 gctccttatc cacgccatca cattcttccc ggatctcctc ggaaagaccc ggttcatgaa
    15061 gatcttctat tagaggcgcg ggtatggctt tatccttttc ccaggcttca ccccttgtca
    15121 cactctcccg gaaatcctgg aaatcatcca catcgatgag cagttccccg gtaccgggcg
    15181 atgtgaccct gactctcctc gccgcaacat ctatgccgga aaccacgtag ttgacggagg
    15241 gcgtttttat cttcgaaccg gggctcggca ggttcttcca gagttcccgg tatatctcgt
    15301 gctcgtagcc catgcagcac atcagccgtc cgcatatgcc cgatattttc gccgggttca
    15361 gggcgaggtt ctgctccttg accatcctta tgcagatcgg ggcgaacctg ttcagccagt
    15421 aactgcagca gcagggcatc ccgcagggtg agatcccctt taccactttt gattcatccc
    15481 taacacctat ctggcggagt tctatcctcg tcttgaactc cttggccagg tctcggacat
    15541 aggccctgaa atcaactctc tgttctgccg tgaagtaaaa gaacaatttt ttcctgtcgg
    15601 gaaggtactc gacatcgacc agtttcatct ccagatcgtg ggccttgagc agttcgcgcc
    15661 ccttagccag tacctgggct tcctcttccc tcaaggaggc gctttccctt tcatcctgga
    15721 gtgtgaccgg ccgtaggtat ttcacatcct gaaaggccgg ttctccgcct cggggtgccc
    15781 cgtcaccccc gttgttggtc agggtctctc tatacgtttc agcctgctcc atggttatcg
    15841 ggccggcgac aacccctatc tcctcgcccc tggcggaaga aatgacgacc tccatgcctt
    15901 tatcaaggtc cacatcatcg ctgtggagca gccccaggta acggggttta ccaattatca
    15961 ccaagtagct cttcaagtcg cacctctcct tcaagactca ccaaaacaag gtcctgggcc
    16021 atgggggtgg tcaacttgcc cttttccgcc atgagacgga tcgtatccag gcccgctgcc
    16081 ctacaatggt ctcccttgcc caggaaaaac cccacccaac cctggatctc ggcggcgagg
    16141 accttaggct ccagtttcct ggtcttcccg acccatttta taaattcatg aacaccggga
    16201 ggggtggcct ctcccccggg ttcagggccg acacttccgc gaaactgaaa gagtctcaac
    16261 ctgcttctca gggtgggaat gagcacgttt tcctccagca aaaagatcaa ggcccccgtc
    16321 gaaggcgtct cctcggatat tttcagaaga ctgttcgccg ccgggaggga gagcttgtgg
    16381 gacgagtgga taacggctac cctcttcctg gagatgacgg gtttcaggga aagttcctcc
    16441 caaagcttcc tgcaatcctt gatgccggga ggttccccgg gggccccaaa aaagagcagg
    16501 tccggttgtt ccgttttgga ccaagccctg caggcggtac aggtcccgca accgtcgccg
    16561 gaaaagcaca ggatcttcct ggcgcaggcc atggcaaaat ccatatgtaa cgaaaaaggc
    16621 attacaccgg ccacaggtag gcgtgcccca tcagatccta ccacggagga aacctcgtcg
    16681 acgaaggagg aaaggttatt ctccaagttc tttgcctccc tttccttctt gcctcccttc
    16741 atcgtttcgc caaggttttt caccgaaggc tccccttttt catcaaaagt gccatgatgc
    16801 ttgatgccat ggtatccacc agttcgtcgg gatccttagt gccatccatc ctcttgaatc
    16861 ttgccggctc ctcgtggcaa agggtcccgt aaccgagccg gatgcgttcc aggagtggtc
    16921 cgtccttctc aaacctgtcg ggtttcccct tcctggcaac aattcttccc agtgccaccc
    16981 tgacaggaac atcaagccaa aatgtcaggt ccggcctggg gaaacggcct ccagcggaga
    17041 tctcttccac aacccccctg gggaaacttt ttccgaaaac ctggtatgcc aaggtggaat
    17101 cggagtacct ttcgcaaagg atcacttttc ccgcccctaa ccccggccga atgacccgtt
    17161 cgacgtgttc agcccggtcg gccagaaaaa gaagcgtctc ggtcagtacg ctcttgaggt
    17221 ctcccccgat gagggtctcc cgcagcacct ctcccccatc ccacccgccc ggttcatggg
    17281 tcatgaccat tcggtccata ccaagtctct ctgccagttt gcgaaagacg gacccggcca
    17341 gggtgctctt tccggatccg tcaattccct cgaaggtaat aaataggccg agggaggggt
    17401 gaccctcatc aaaagaggta gaagaacccc aatgacccga cactgtcatc acccttatcc
    17461 aggtagcgat agaaaatgcc ccagctttta tcaaaacgat accggccctc gagggaccag
    17521 ctccctccgt tttcgttcca ccactgggcg ataacggaaa agggaccttg gggtcctacg
    17581 gggtgcaaga ggaagcccgc cccgggatag cctcggacgg ccccccccca gaattcccat
    17641 cggtcaccgt ggagtcccag gccggctgta accttggggt cgcctccgat atcatcggcg
    17701 ccgatcagga gacccatctt cctggaacgg cttttgaccc aaagggagat gtcacccatc
    17761 atgtcgcttt cgcccgagcc cgatattccc ccgtacagtc cgaccttgcc tccaaaagag
    17821 aggtcctcga tgtcggagat gatcctgttg gctttcccca ccatttcttt tacttcctgt
    17881 atccgtgaag gcgccggcgc cgtatcgtcg aaaagggcca cctcgatctt tctggataga
    17941 gcctcgaggc tttccgccgc cccttttatc cttaagatca tctggcgaag atcctgaccg
    18001 gatgtacccc cttcatcgaa ttggtctacc acctttttca ggttgcccag gaactgggaa
    18061 aggttttcga gcgacgccat aagggcgccc ctattctcct ggaccatgga gtcaatattt
    18121 tcggtcaggc ctgccagcga atcgccggcc tttttcacct ggtcgcgggc ttccttggaa
    18181 aactcctgga attcaccggc ggcttcggaa agttttgcaa ggaaagtgtt tatatttgaa
    18241 aaggtcgact ggaggccgcc gaggctggtc ttgatatccg tcattatttc atccagcgag
    18301 ggcggtatgg tccctgacac ggtgtctccc tcggttatgt tttcgaggga gtcgcccctg
    18361 gtgaccttga cacggggttc acccaagagt ccgcctgtgt cgatgataaa acgactgtcg
    18421 aggggcacaa tgacatgact tcttacattc acccttacca gcacaccctc ttccagcaag
    18481 tctatcccga cgactttccc cgattctaca ccggagacga ggacgggagc gcccgtgtcg
    18541 agacccatgg cgtcggggaa gactatgccg aaagaataac catactgtcg gaaacgagct
    18601 cccccgctga caaagacaag ggctcccaga aagacaatgg ctaaaaagac cactagcccg
    18661 actctcatct ccctgttcac atcttctcgc ctcctgtcct cccgacggat accatccctt
    18721 gggcgaaact cttcaccatg ggatgatcgg acctttccca ctccacgggg ataccccgcc
    18781 aggcgatcct gccttcatgc atcagcaaaa gagtgtcccc aatgcccagg gcggaaacga
    18841 tatcgtgggt cactaccaac gcggtgaaac caaactctcc gcagagatcg accacgagct
    18901 ggtcgatatg gcgggccgtt actgggtcga gtcccgtagt gggttcgtcc aggagcagga
    18961 gggacgggct atgcacgagg gacctggcga tggccactct ttttcgcatg cctcccgaaa
    19021 gcatggcagg atacttgtct ctcacatcct ccagctccac gtgttcgagc atatccagga
    19081 ccattttcga gatcgactcg gggtcctcga tctccctgca atacttcagg gggaaggcga
    19141 tattctggcc cactgacagg gaatcaaaaa gagccccccc ctggaagacc actcccagtt
    19201 cctttcttac ctcgaccagt tcatcctctt gcgtttcggg accgatcacc ttgcccaaaa
    19261 gctcgatccg gcctttatcg ggcttcagaa ggcctgaagc caatttaagg gccgtagtct
    19321 tcccgcaccc ggaaggtccc atgaggcaag tgatctcacc gacgggaagg gtcatgtcca
    19381 tcccgccgat cacttctctc ccgtcaaagg aaaccgaaac tccctgcatt ctcatgaccg
    19441 ggtccatggc ctcacccctt gatccctccg aaaagcaggg aagagaggac gtaattgaag
    19501 accagtatta acatattacc ccagacaaca gccttggtgg tggccgtccc cacgccccgg
    19561 gcaccttggc cggccatgaa cccctgaccg caagcagaga caacgatgag tacaccgaag
    19621 actgcagcct tcaggagtcc cccataaata tcggaggggg cgaggagcgc ctccacggac
    19681 cttctgaaaa ggtgggcgtt cactccgtga gccgcgacat acaggaatcc cccggtgatg
    19741 ccgatcacaa aggagaacag ggtgagcacg ggcagcataa ccaggcctgc caacaaccta
    19801 ggcaccccga cgaagacatg gggatcgagg ccgaaggtcc tcaaggcgtc gatctgttcc
    19861 gtcaccttca tagaaccgat ctctgcggcc atggccgccc ctatcctacc gctgaccacg
    19921 agaccggtaa gcacgggtga catctccctt accatgctta gggcgatcac ccctccaata
    19981 tagccggtcg cgccgaaacg gacgaattgg tcaagggtct gcacggccaa gaccatcccc
    20041 gtgaaggcac tggtgatgga taccaccagc actgaaccaa cgccgaccct ttccagttgc
    20101 tcgaaaagat cctccctgct ccaggtacct gagaagatcc ctgtaaaagc ctttagaccg
    20161 acaataaaaa agaaacccaa ggtggaaaaa agctctatgg cctgtcttcc gatccatgaa
    20221 agaaggttca aggccccacc tccttattca gggatcacct tgatcattct tctttgaagg
    20281 ccatcgacaa aataatttga acggtagctc ttgaacgacc cctctttgat actttcttcc
    20341 ttgacctggc tcctgagttt cttctgactc tcttcctctt ccggggatag ggggtcttcc
    20401 ccaggcaacc cgatacttct cacctcggcc acgacctcgt cgttggtttc catcgccgag
    20461 aattccttca ggagctcatc gaggctcctg atggtcctcc aacccatgat gatcgactcc
    20521 tggtcgggct cttcgatccc acccccccgg accaccactt cgcatggacc cttcgcgtcc
    20581 atgggcacct tgagggtcat cttgtgggtc actgtttcct tcctgaaggg acggagagtg
    20641 acctccacgt cgaaactccc tcctggaggg accgttttcc gggaaaggtt cacatcttcg
    20701 atcagtacga ccctgggttc agtcgtggcc tcgatgtcaa tatgcacccc gaagggggcg
    20761 atctcgatga aggggttgag cgatacagcg ttcacaaggt ccgagaactc ggagaaaagt
    20821 atcttggtca gatcggtgga agaaaagaac atatttgtcc tctgccaacc ttcggccagc
    20881 ctccctccct ctatcttcag ggtgagcttg gcgctgcctt ctccaacgcg accccaaacg
    20941 ttatcaatga ggcccgtgat catttcgggg atgaccttgg tcatcatgaa ggggtcgcag
    21001 gccgtcttga atctcttgaa ggtctcggtc ccggaatcca catctcgaaa attcagggag
    21061 atgtccatga ccggggcgaa acggccgata aaacccccga tggcctgggg tcggtcctgg
    21121 gtgacgatcc ccacgatgga tgtgggtgta ccgatcttga aaggcgtatc gaggctgggg
    21181 accacctgat ggatccagga tcgggcaagg gggaaggcca ccgccccacg attggtgaaa
    21241 gggtgggcaa aagcgagaaa acgcccgtcc gtcgacaagg ccgtaagcgt gcccgtcgcc
    21301 gctaggacca catcgcccca ggcaacaaga acgctcacgg cttcgccggg cctcaggatt
    21361 ggtttgtact ccactgggag tgacaggtcc ccgcccccgc ccgggagaag gatgaccgga
    21421 tttcccaggg catccgcgat cttccttgcg ctcctggagc tatagccgct accgatcagg
    21481 ggggctgaaa gcccaccccc atctcccggg ggagacactt tttggttccc gggctctggc
    21541 tcgaaaaggt tcaggtcacc cgaggcggga tcatggagtt ttaattctgg gacctttagg
    21601 gagggtacct tgtcggccca gttccagatc tcagccatct catcgatggg tgtcaccagc
    21661 cccatctggt gttcgctgaa gctccaaccg taacctatcg cgccgaccag cttcccccct
    21721 acgaaaactg ggctcccgct cataccggcg gctatacccc cggtctgttc gatcacgggg
    21781 ccggaagccc tcaccagaat gaggttgtgg gggcttttcg gggagggcat cacgctgagg
    21841 accgtgacag ggaaggagac cacttcactc cctttgatga cggtataggc gacccctttt
    21901 gtcccttcac tcagggaggc ggtagaaaga gtgcccccgg gcacacaaaa ggtcgatgag
    21961 taggcgggca agaccaggca aagacaaacc ggaaaaacca ggaggaagat catcttcctg
    22021 aacatcgagg gacctccatc aatctgtcat cggtgatgct tcctgaacct gaggaaggaa
    22081 aggatgaatt catcgatgtc accgtccagt acggcctgca cgttgccctt ttccaggttc
    22141 gtccggtgat ccttgaccat cgtgtaaggg tgcagaacat aggacctgat ctggctcccc
    22201 cagccgatct cttttttctc cccctggatg gcgttgatct gttcctgtct ctcctgaagc
    22261 atcctctcgt agagacgacc cctgaggacc accatggcgc tatgacgatt catatgctgg
    22321 gatcgctcgt tctggcagct taccacgatc cccgtcggca ggtgggttat cctgacggcg
    22381 gaatcggtca tattgacatg ttgaccgcca gctcctccgg acctgtaggt atctatccgg
    22441 agatcttcag gatcgatctc gatctccgcg tcgtcgggca gttcagggga gacattgagc
    22501 gaggcgaaac tggtgtgtct cctcttggcc gtgtcgaagg gcgatatcct tacaagcctg
    22561 tgtacgcctc tttcggtctt caggtaccca taagcgtact cccctgctac gagtaccgta
    22621 gcggatttga tccccgcctc ctcgtcctcc gtgatctcaa ggatcctggt ctggaaaccc
    22681 ttctcctccg cccagcgaag gtacatcctg aggagcatct cggcccagtc ctgggagtca
    22741 tggccgccgg cccccgagtg aatgctcagg atagcgttgg attcatcata ctcttcatcg
    22801 aggagcagca acagctgaag atcatggatc cttccttcga gggctgtggc cctgcgatcg
    22861 aactctcctt cgagttcttg atcctcgccc gatccgagga gttcggtcat gacctggagt
    22921 tcttccattt cccgggagac tcccttccat gcgtcaagcc gtccctggag gcgggacaac
    22981 tcccttgtga cggcctgggg gttttcgcta gcccagaagc cctctccgga agttatcttc
    23041 gtcagttttt cgacctccac cgaaagttct tccgggtcaa aggctttcct tcaactcgga
    23101 aacaagcgag tgaagtccct ccattacggt ggtcatggga acctgggcca cgcggttcac
    23161 ctccaaatgt ctttgaagtt ggggagtgct attattagta attcaggcat ttcattatat
    23221 catccagtga tcccctgggg ggaaaggtat gcggtcatgc gccccttttc gaaaaccaag
    23281 atagccgttt tgagcgaact catgggagcc ggagatctga ccgtctcggg gggggccctg
    23341 tgcctttcac tggggcttac gagacaagcc gtttggaaag ctgtgaatgc actcagagag
    23401 gacggttatg tgatcgaatc gatcccccac aaaggttatc ggttcgtatc gcttccgaag
    23461 aacgacctag acccgtcatt gatcgaggcc tttctgttgg actgtccctg gggacaccct
    23521 gtcctttatt gggagagtct caactccacc caggtccccg caaaggaact ggcccgcaaa
    23581 ggcgccccgg aggggctgat cgtcaccacg tcctaccaga cttcgggcag agggaggctt
    23641 gggcgacggt gggtctcggc accggagggg gggatctttt tttccctggt gatccgacca
    23701 gcgcttccgc ccgaatccat ccaattgcta agcctcgtct cggccatgtc ggtccaggat
    23761 gcccttttca cccttcacgg agtgaggtgc cagctgaaat ggcccaacga tatcctgtgg
    23821 gaaaactcaa aattgtgcgg cattctcact gaggtttcca gcgaacctgg cctggtccat
    23881 ttcgccgtca ccgggatagg tatcaacgct aactcaaata atgtctcgat cgatgaaggg
    23941 tccgaaacgg tttccctttc atccatcacg ggtaaaaaaa tacaccgggg agagttgatc
    24001 gccggggtgg ttcgctacct ccacggtgct gtgagagggt tggaagcccc tggcggtatg
    24061 aaaagggccc tggacgagta ctcgtccagg tgtgataccc tggggagaaa ggtccgggta
    24121 gtttccgacg agggcgaggt tatcggtgag gccgtcgcct taggggagaa gggagagatc
    24181 atcgtccgga ccaaggacgg gacccaaacc ttcacatccg ccgacgtgaa ccacttgagg
    24241 gctctcggct gatcactcaa gggttcctcc tcaggtgatc ccatatcttc aggcagtggt
    24301 cgtagaggaa aagaatgata tcgtcctccg accttggagg ggaaagctct acgacccttt
    24361 tcgaaccttc gagcctttcc ctgaccgtcg ccggggtcca ggggtcgatc acgatgaccc
    24421 acccgtgcct tccggcttct cctagggccg tttcaaggac atccagcgcc tgtgagcggg
    24481 accacttctc cttgacatcg gcaggaggcc ttactgtctc cccggatata gcctggagcc
    24541 acctcccata cttccagcaa agatcaagga tcgttttgtc cccgatcatg tttttcccaa
    24601 cgatcaccgt gctgtcgagc ctggcctgga attcagcgag cctccgctgg aaatactcaa
    24661 aatcccccgg cccgctgatg gaaaggacct gaagggtacg gagcccaata aagggcaacg
    24721 tcgcgggatc gaggaagctc tcgtcaaggc cggatgaaca gggcgacctc tcaaagagat
    24781 aaaaagtgga gtatttctct tcgagccctt tgccaagaag cgggactccc tcgcccttca
    24841 tcactgaaaa aacgaccccc cccgccggtg gggaggtccg cgccttaacc ggctgcccac
    24901 tctcatccca ggtaatgacc ggatgaaccg tcacctgtac gccccctata aagcgtgtca
    24961 acatggcgag ccaggggtcg gtgacccaaa tatcaagagc ttcgatcctt ccggcgcaaa
    25021 gcgacgaaag gataacaagc gaaaggacca aggttttgga aagggtcagg aaagcctcgg
    25081 cagtatgttt cttttcaatt actggggaac gttctccggc cggagtgatc acactttacc
    25141 cccgtcccag cttgaaagcc tcctgctccc cggttcggag agggggatgc cctcccggca
    25201 caaggggcat tcttcccggt tataattctg gaaatcgaaa cgaacaaggg aggttaaccc
    25261 tttttcttca atgaacaggc cgtcgctcct gtcggcgatg cagccggcgg cgaggaaacg
    25321 cgtctccggt acggagtcca tcactttttt tacctcgagg accgatcttc ccgaggtcac
    25381 cacgtcttcg ataatgacga agcgggcagg accgggggcg ggaaaccttc taagtttcat
    25441 ctcccccctc tctctttcgc aaaaaagaaa gggtactcca aggtgttttg ccacctcgtg
    25501 cccaataatg atcccaccca atgccggcga gaccacgagg gacggctcca ggtgggataa
    25561 cttccttgcc aggaaggaac cggccaaggc cgcgttttcc gggtacatca ggaacagggc
    25621 gcattgaagg taatgaccgc tatggagacc tgaggacagg gcgaaatgac cttcctgcag
    25681 ggcacccgat gctctcaaga ggcggacgat accctccatt gactccattg aggtgtccct
    25741 tctagccatt ggtcttcctc caaaggtcaa gccgttcagc aaagcgtgcc atggctccca
    25801 ccagcgagga ggactggatc acgggcctcc cgatcacgac aaagtcggcc cccatgtcca
    25861 gcgcttcctc aatcccggcg gtccttttct gatcgtcgcg gctgacctgc ccgtcccgta
    25921 tccccgggac caccctcaag aatcccacgg ggagaagacc gtccaggagc gggaggtccc
    25981 gcggcgaaca aacgataccg tcgaaaccgc attccttgca gaggagagcg cggcatttta
    26041 ttgactgccg cagttcggac ccgggggacg atcggtcgag accgcgctga tcgatgctgg
    26101 tgaggacggt aacaccgagt agcaggacct cggaccgggc tccccccttt gccgaaacgg
    26161 ccgccttgag catctcctct cccccggagg catggagggt caatgcccag ataccgcttc
    26221 gagcgaggag atccaccgct tctgagatcg tattgggtat gtcgtgaagc ttaaggtcaa
    26281 gaaaaacttc gaaaccggcc ctgattacac gcctcacaaa gggcatcccg cccgccgtga
    26341 aaagcctggg cccgatcttg acgtagcgaa gatgggttcc aagggtttca aggatccttt
    26401 cggcttcgct gggttttctg acatcgagcg ccaggattat gcccggcccg cctttcttca
    26461 atggcgactc accccctctt cttccttttc acccccgggc ccgcccgacc aagtccccta
    26521 cggaagaaag gcctttcttc cgcagatgtc gctcaagttc ccggcaggtt gacgccggaa
    26581 gtcgggaatc cacgaaaaga gccgtcccga cctcgacggc tgaagctccg gcgaggatca
    26641 tcgaaagggc gtcctggggc ctcgagatcc ccccacagcc tattacgggg atctcaacgg
    26701 ctccagccgc ctcccaaaca gccctgaggg ccagggggaa gacggccggg ccggaaagcc
    26761 cggcgaagac gttctcgaaa aaaggcctct ccctttcgtt gtctatggcc gtcccgagcc
    26821 aggtgttgcc caccacgaca gcctcggccc cacatccctg gctcaactcg gcgaattccc
    26881 ccactgaagg ggcctgggga gacaacttga cccaaaggat gccatcccag gccctcctgg
    26941 cggccgtgac cgcgctctcc aggctatcgg ggtggatccc ccaggtcatt ccaccccttg
    27001 agacgttagg acaggacacg ttcaattcca gggcgctgat ctttaaggcg gatcctttca
    27061 gtacttctac ggcttccacg agatcgtcga gggtctcgaa ggcaatgttc acgatgatgg
    27121 gcctacccgc aaagctgagg gcaggcagtt cttgggccac gaaatgatcc acaccggaat
    27181 tctggagccc gatgctgttc aaaactccgg aataggtctc cactatcctg cagcccgggt
    27241 taccatccct gggcttaagg gtcaaacctt tgctgcaaac ggccccgacc ccatcgaggg
    27301 aaccctctgg ccaaagcgcc ggatccatgg gccatacccc tgaagcaact attaccggcg
    27361 tatcgaggtc aacgcctccg acttccgttc taaggctagt tccgaagggg ctcaccaaaa
    27421 acctcccgcc agtcgaagac cggaccgtcc acacaaaccc ttttcatacc cgatggcgtg
    27481 gttatcgaac aactcaggca accacctatc ccgcagccca ttcttctttc caagctcacg
    27541 agtactttct cgcaaccctt ttcgtatgcc cttcccaact cccgtagcat ggcttccggg
    27601 ccacaccccc ataactcccg acccggcccc accagggtga gtgaatcttc aacgggatcg
    27661 ccccgccttc caagggaacc gtcatcagaa ttcagcctta ttccagggat cttatcttcg
    27721 caccattccg cgaacggttc ccagcccctg cctggcaccc cgagcaccgc cgtaacatcg
    27781 accttctcgc cgagggccga aacggccccg aggaaagggg ctatccccaa cgtaccggcg
    27841 accagcaaaa gtttttcccc gacgggtgct gggaacccgt tcccacaagg tcctctaagt
    27901 tccagagctg ctccgggcgg cagcgaagcc aggcggcttg tgcctctccc ggtcacctgg
    27961 aagaggaact caagccgacg gtcccctgaa gccgttgagg cgagggggcg gccaaggagc
    28021 gggtcaaata ccccccactc tcttaccaag aaaaactgcc ccggtgccga cggcgcggtc
    28081 gcctcgctca gctcgacagc gatatgcctg caattttccc cgaccttccg ggaaaagacc
    28141 agggagcctt tcctgtcagc cggcgaccct ggttttccca ttcctgaatc catcaatacg
    28201 aacaactcct tagatcgagc cattaaagga atggacgatc cgacccccta ccaatgtcat
    28261 gaccggccat ccctggaggt gcttcccgtc ccagggtgtc agcctcgcct tgctctccca
    28321 ttcatccacc cggacaacac gttcctggtc gaggtcaatg acggtcaggt cggcgggccg
    28381 cccctcttct atctttccca acttcttcca tttcccggga aggatgctcg ccggtcccga
    28441 ggtgaagagg ttgaggatcc tttcgagagg gcaaggtttc cctctccttg cccattcgtt
    28501 caacacggcg gccacggcgc attcaaggga ggctatcccg aaggcggcct cttgaaaggg
    28561 caagtccttt tcgtccagat gatagggcgc atggtctgtc gcgatcg
//
LOCUS       DAOSRG010000002        22611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_2471_length_22611_cov_8.908450, whole genome shotgun sequence.
ACCESSION   DAOSRG010000002 DAOSRG010000000
VERSION     DAOSRG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 22611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22611
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_22611_cov_8.908450"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLM30_00135"
     CDS             <1..947
                     /locus_tag="PLM30_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOR53608.1"
                     /translation="WDRVTDPVRVARVTMILSRLTPFTERDLPFEVRLVEEKRPNAFA
                     LPGGRIYMTTGILEFCRSDDEVASILAHELVHTDRKHGMIQSSRNQRLTLGALAVIIA
                     TGGSGAAPILANLAQVAIMNSYSKDLEREADTEGLNILSQSGYEPAGAVTVMERLQEE
                     EMKRPYVDPGIYADHPKTKDRVRSLEDIIREKGWPLERKKALRLLRVQTSEEGGSVLL
                     SIDGKPLWRAPKVEGAAELMNKAAGSLDKYLQMETSPYDISIQETHGQKALRVGLGVI
                     VYEKDLLPGMPSLEEFRENILLSLNRARMVHPVADYNR"
     gene            987..>2427
                     /gene="dnaX"
                     /locus_tag="PLM30_00140"
     CDS             987..>2427
                     /gene="dnaX"
                     /locus_tag="PLM30_00140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR53609.1"
                     /translation="MYLSLYRRHRPGVFSEVVAQEAAVHLLRREIASGKNSHAYLFSG
                     PRGSGKTTLARIVAKAVNCPDRDEEGEPCGQCPSCVSIASGDNLDVIEIDGASNRGID
                     EIRELKEHISLSPFSGRFKVYIIDEVHMLTEPAFNALLKTLEEPPRTVVFILATTEPH
                     KVPATIRSRCQHIPFHRIPLPAIVETIRKVSASEGVHIEEKALWEIARESDGSLRDAL
                     SMLEQAVTTGLPSVSLEEVRGILGGGGRSDLERLLPALRDGSPEAFRGFSELFQRGLT
                     AERFLEGLFLLFRDILVAVLWKEEGIDSLPLNEEERSFVRSEAGHWREPDLWKALDLC
                     SATLPRARYGLKGDVVLGMLLGLFLAPGSKRERIERKKEEPLSEGEFPVKKAAGPEAM
                     GTVEGSLPPVKIETPEDTPTPTSSTGAGGWGDCVAAVAADNLPLYCACASSNCRKDGD
                     SLVVVFPEEQRFSFELATSPRNLAYLDSIR"
     assembly_gap    2428..2527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2528..5605
                     /gene="dnaE"
                     /locus_tag="PLM30_00145"
     CDS             <2528..5605
                     /gene="dnaE"
                     /locus_tag="PLM30_00145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR53610.1"
                     /translation="DGFYYRPRVDHDLLSRYSRGLIASSACLAGEIPSLILEGSEEEA
                     LQRAMLYRDIFGEGNFFLEIMHNGIPEQAVANRALIKMSSQTGIPLVATNDAHYLEAK
                     DATWHDVLLCVQTNATVNAPSRLRFGADDFYLRSPEEMWSLFGNDVPQALENTVAIAE
                     RCNVGFSFGSYRLPRVEVRKGSSLEEELSIKAREGLQARFSGDVVPAGYGERLDYELQ
                     VINQMGFAGYFLIVSDIIHTAKEEGIPVGPGRGSAAGSLVAYSLRITEIDPLRYGLLF
                     ERFLNPERISMPDIDTDISDRGRDRVLEIIVEKYGRSHVAQIITFDRMKSRAAIRDVG
                     RALDMKYPDVDRIAKLVPSGAASIEEALQQSPDLKQARKEDPSVSLLLDYAAKIEGLA
                     RHCSQHAAGIVITPEPLTDVVPIRRIGEEQIVTQYSMEPLESLGLVKMDFLGLRTLSM
                     IEEALSNIRRNGKEAPDLYSIPSGDRAAFDLLWRADTLGIFQLESSGMRQLLKQVRPD
                     CFEDLIAALALYRPGPLGSGMVEQYIDCKHGRREISWLHDDLKEVLRETHGMFLYQEQ
                     VMQCAATLAGYTLGEADLLRRAMGKKQADVMETQRKQFVEGCAGNGVKASDAEHIFDI
                     IQEFAGYGFNKSHSAAYAMITYQTAYLKAHYPCEFYASFLSSHIHSKLDVLANHIRTV
                     RNSGIKVLPPDVNRSEANFSVDRDVILFGLGAVSKMGQPTVEQIIGEREAGPYLSLWD
                     FINRVDTRVVGRSVMENLIRAGAFDSITPNRRQLMESLPGMFELAQRRNDDCHSQQLL
                     ELEDLSEEEGPVLFEVEDYDLHTRMEMEKEATGLYISGHPFDKYEKEVLPYTTCSITD
                     LSFWRSEDCTAVVAGILAGVQEKFTRKGDRMGILELEDSSGKVEVVCFPRTWSSITPP
                     EKGKAVVVKGSPRTRDGLSLIAEKVMAVDELDGSTRKWLRLRVLDDGASSNGRLRDIY
                     RELKGHPGEAGVLLEVPLEGVKALLKVRDVFVEPSADLSTRISELSGGCVEVV"
     gene            5651..5911
                     /gene="mtrB"
                     /locus_tag="PLM30_00150"
     CDS             5651..5911
                     /gene="mtrB"
                     /locus_tag="PLM30_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HOR53611.1"
                     /translation="MPVEKEDKPSVLSDFVVVKALENGVSVIGMTRGQETKFAHSEKL
                     DAGEVWIAQFTEFTSAMKIRGRAHILTAHGVVESGRKNEETG"
     gene            5895..7664
                     /gene="pyk"
                     /locus_tag="PLM30_00155"
     CDS             5895..7664
                     /gene="pyk"
                     /locus_tag="PLM30_00155"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOR53612.1"
                     /translation="MRKRAKIVCTLGPACEDHEVLKRMVQAGMDVARLNFSHGDHGTH
                     GSHLDKVRRIEEELGRPIGTMIDTKGPEIRTGTLEGHRPMMIKSGSTITLTAEPLEKG
                     DASRVGISYPGLPGEVGPGQDLFIDDGTLHLIVEKVEGKDITCRVNVGGELGEKKGLS
                     IPGVKMSLPILTPKDIEDIRWAVEKGMDFLALSFVRDRSDVMDARRVLEEIGGDLRVI
                     AKIETRDAVDNLEEIIEVVDGMMVARGDLGVEIPLEDVPLTQKAIIDRCRSHGKPVIV
                     ATQMLDSMIRNVRATRAEASDVANAVLDGADALMLSGETANGAYPVLAVKTMAGIIGS
                     TEKGGSPWRKPFIFQTGAPGIPDAVSKAAGMISDQMKAQAILSLTQSGVTAQMVSKYR
                     PAAPIIGATPNRETLRYLTLVWGVEPLLVPEEKVLETALTSAMETARKESLLREGDMV
                     VATAGYPIGSPGSTNSIQVLTVAKTLLKGLSLLKKDVSGVVVKALTGSEADAKMKDGA
                     ILVTRQTDKEYIPAMRKASAIICEEGGLTSHAAIVSLELRIPCIVSATGALAALEDGM
                     TVTVDGRRGVVLGGAVRLHSDGD"
     gene            7699..8280
                     /locus_tag="PLM30_00160"
     CDS             7699..8280
                     /locus_tag="PLM30_00160"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOR53613.1"
                     /translation="MKTPSHRLDIGWEDITESQGRMSAVLVPLFPRGEGLFLLLVERS
                     DSLKRHPGQVAFPGGAREKRDRGPVETALREFTEETGIPAERVEVVGALPEVWVYSSD
                     FTIFPVVGYLPSGVAPGDLSPDGNEVKRVLEVPLKQLERPPRMENPAGEGQNFPFPVY
                     RLDDGVILWGASARIILDLTRSLCDSKGGTRCP"
     gene            8271..9113
                     /locus_tag="PLM30_00165"
     CDS             8271..9113
                     /locus_tag="PLM30_00165"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOR53614.1"
                     /translation="MSLIGAHVSVAGGLWKAIERGESLGCEAIQIFTKNQLQWLTPPV
                     SESVASRFMAAWGRSTIKKVVAHASYLLNLASDTEKREKSIAALVEEVARCEALGIED
                     LVLHPGSHLGRGLEYGRQRAAEGLARALERTAGMRTRVLLETMSGQGHSVGSTIEDLA
                     WILERVGDPSRVGVCLDTCHLFAGGYELRGRSSYDRFVRAVEKHIGLVNVGCWHLNDS
                     TAEMGSHIDRHQHLGEGFIGLELFSRIVADPRWEDIPCLLETPKGLPGDSGNLALLRK
                     LRGR"
     gene            9160..10239
                     /locus_tag="PLM30_00170"
     CDS             9160..10239
                     /locus_tag="PLM30_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOR53615.1"
                     /translation="MTSRYPEMSVDTAMITRNAEKVVGLCSGKGISVWGVTKGLSAHP
                     AVASAMLDGGCVGLADSRVDNIRKLRLFGFRRPMMLLRIPMPGEMTNVAENVDCCLVS
                     MPASVLMLDQACDEAGSEVGIIVMVDLGDLREGIWPDGVEEMADALLQSRRTRCLGVG
                     SNLGCLSGVLPSVTNLSLLVRIGGELSNLLGHPLEVVSGGATSSLSLVEDGTMPEGVN
                     QLRVGEGILLGTDVTGNRVIPYLERDAIRITAEVVEVRRKPSMPIGKTGADAFGNIPV
                     FQDRGMRRRVIVAMGRQDVRPEGLTPVAPGLEIIGASSDHLTLDAEDFEGPLNPGDTI
                     SFAADYGAMLAAATSPYVRTKIIKK"
     gene            10258..11103
                     /gene="cdaA"
                     /locus_tag="PLM30_00175"
     CDS             10258..11103
                     /gene="cdaA"
                     /locus_tag="PLM30_00175"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOR53616.1"
                     /translation="MSGILESIRWQDFIDIIVIAFIVYRLLLLLMDTRAMQLVKGLFV
                     IGLFYATARFLKLAALTWILGRLLGVFILAIPIVFQPEIRRMLEELGRGSIWRRRQAE
                     ERASQLTDEITRALLFLQSRKIGAILVFQKDTGLKDIWRSAVQIEGDVSQDLIISIFW
                     PGNPLHDGAVILDDRKVIAAACYLPLTENSNLSRWIGTRHRAALGVSEVSDAMALVVS
                     EERGEVALAINGHLSRELKEGQIRRLLLHYFTRAAGPATAVEGIRERLGSVFSRGGSD
                     NDEID"
     gene            11087..12298
                     /locus_tag="PLM30_00180"
     CDS             11087..12298
                     /locus_tag="PLM30_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOR53617.1"
                     /translation="MMRSIDRLLASRLFLKVLSVLVAVLVWFYLASDRGTEVVRTVTV
                     PLEFLNVPADMSVTSGVRDVDIQVSGTREDTLLKMDTIASQVDLKGLGPGSYRRPIQA
                     ILSSGLRLVEVSPPFVDLDLTRLVSRTLTVKVLVPEDLPPEYHLEEERVEPREVSVKG
                     PEQMVASLDNVWVAPTVEQLLQEKELSLSVIPFPGSDQRSPFMIEPAVADLFFRLIKG
                     FPRKVVPVRVELKGEPGHDFQVEAVVVDPPELTIQGPFESIERIYQVNLAPIDITGLA
                     SDVSEVVTAPDPADDAQIVDVPSVRVRILLAPRMERRLFAKIPVRLAGRSIYPGWRVE
                     PSEVNIILQGPPSVLDSAEAEGSPLDVFVDVTNLVSQKITVPVKVTLRARGATLAEVD
                     PPNVTVYALTE"
     gene            12321..13727
                     /gene="glmM"
                     /locus_tag="PLM30_00185"
     CDS             12321..13727
                     /gene="glmM"
                     /locus_tag="PLM30_00185"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR53618.1"
                     /translation="MLVPLGKSRCLFGTDGIRDVANRGLMTPEMALRLGRSYALFLIE
                     RGVSRPTIAIGRDTRRSGYMIEMALVSGLLSAGAEVASLGVIPTPGVSFAVKRLSCDG
                     GAVVSASHNPFEYNGIKFLGKDGGKLSDEEEAEIEDYLADVVFDEWRPTGSSIGSVSP
                     RPEILHEYTENLVRTCCLGADPELSIVVDCAHGAASEAAALVFGRCFKKAALISDRPD
                     RYNINDGCGVIHIDNVATSVLSEGYSLGMALDGDADRVLFVDPRGRVIDGDIILWILG
                     RWLLSEGKLGNGVVATVMSNKALEEKLSQEGITLQRCPVGDRYVLDAMRLSGSNLGGE
                     QSGHIIAGDFFPTGDGLGSGLLFLSACARLGEDTDTLVDRFERYPQVLKNFHTRDKDA
                     FLGHSEVREAISKASFDLGDLGRILVRPSGTEPLVRVLVEARDDTLLEAVSGTLFERI
                     AKVASSAAFGSLGGGEEP"
     gene            13727..14659
                     /gene="galU"
                     /locus_tag="PLM30_00190"
     CDS             13727..14659
                     /gene="galU"
                     /locus_tag="PLM30_00190"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOR53619.1"
                     /translation="MGDRGRKCLFPVAGMGTRFLPATKEIPKEMLPLIDKPIIQYGVE
                     EAVRSGCVEIVFVTGRGKRAIEDHFDRSIELEQLLLKKGKADCARIVREISEMADFAY
                     VRQSEPLGLGHAVLCGEPFCRGEPFGVILPDDVMISPPGSPPVLEQLFLVHERLGGSV
                     VALEEVPLEEAGRYGMIKGEPVEKGLFRIEDLVEKPRPGGVPSRYAVMGRYVLSPKIF
                     RHLKSSAAGAGDEIQLTDALKVLAKDEPLWGVLYEGERFDCGTMENWLKATLGMALGR
                     EDLRDIVLCELKRLGVLEGGDGAAVSEDGNVRSS"
     gene            14839..16671
                     /gene="glmS"
                     /locus_tag="PLM30_00195"
     CDS             14839..16671
                     /gene="glmS"
                     /locus_tag="PLM30_00195"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOR53620.1"
                     /translation="MCGIVGYVGGREAREVLIDGLKRLEYRGYDSAGLALGSGKDLLI
                     IKEVGKVEDLERMLQKVPAGSRQGIGHTRWATHGGVSQANAHPHKDGSGRFALVHNGI
                     IENFSELREELEREGVVFLSETDTEAIVHLVAASFSRTGDILETLVYLQKRLRGTFAL
                     ALMQQGFPDRIYTIRRGSPLVLGSGDGEAFCASDIPAFLPYTRRIQYPEEGDIAELSP
                     GGIRIWDENGRPVERPAVTVDWDVSMTEKSGFSHYMLKEIHEQGTVLRRSLKDRVVDG
                     KTMISGELGWDDEMVSKWKKIHIVACGSSYYSALVAERLLEKWTDLEIKVDIASEYRY
                     RHVACDPSTLAVFVSQSGETADTLAAQRKARAGGAHCLAITNVRGSTLAREVHSVLLL
                     KAGPEIGVAATKSFTGQLAALFLVALQLGSMRGSLAPHEEERLVNELVRIPYKVEQLL
                     EKEGEIKEVARKYARNKNMLFIGRGMSFPIAQEGALKLKEVSYIHAEALAAGEMKHGP
                     IALLEPGVPVLAVIPKDSLYEKTLSNIQEVKARNASLIAVCTEGDRFVPEHAGSVIET
                     PWTDEEFTPFLSVIPLQLLSFHIALILGREIDQPRNLAKSVTVE"
     gene            complement(16678..17772)
                     /locus_tag="PLM30_00200"
     CDS             complement(16678..17772)
                     /locus_tag="PLM30_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53621.1"
                     /translation="MLLLLFLAADLPPAVASAAALPPSTGVLDGSFTITVELTPGGEI
                     TVQREDGQKTVLGRVKALPANSRWPSFTASAWGREQSVVASAVNAIHILLSVEKGKGR
                     TISLLPEETIAPAAGPGAAIVTDIKAGRALFGAWAPPVGSRVVFEHSDDDPYLVEDVP
                     RKGDVMKIMTDPLPCPYFVELENRPGGRVTAWYGDGVRVLGRVIRPLGGTGRFDGTIF
                     QDTGRIRANHPGVIDVCTSPEGLVGGFQIIPLEHAFSREMLGAWKMTQWMIIGPEMGK
                     VDLKGSGPLFSGGLLPGPARGEVLWDLWSSYGRKPLVLARLKGGPWRKMPALSGRQDH
                     ALNDITHMRIYYPFTREPLRDGTDPSHQLQ"
     gene            complement(17822..18088)
                     /locus_tag="PLM30_00205"
     CDS             complement(17822..18088)
                     /locus_tag="PLM30_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53622.1"
                     /translation="MFRIPLALTVFFFVTSVLLGVLVSRSGSRVRPSAFWGSLLFFLL
                     FSSLTLFFCAPLWIALAPMVPAAIFALLYFYPKKAGWNDPSRNR"
     gene            complement(18092..18550)
                     /locus_tag="PLM30_00210"
     CDS             complement(18092..18550)
                     /locus_tag="PLM30_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOR53623.1"
                     /translation="MWTVEDGIERIRSRGLKITSQRIAILKLLEDRKDHPSAEKIFRE
                     MKEIYPTISFATIYSTAQILRDAGLLQTLSIDDRRVFFDPDPTPHAHFLCSKCGALED
                     IPLEKEILNRIRRSVPHNLDAVQVYCYGTCRACSGREMTGARPRKAPRAH"
     gene            18698..18772
                     /locus_tag="PLM30_00215"
     tRNA            18698..18772
                     /locus_tag="PLM30_00215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18731..18733,aa:Gln,seq:ttg)
     gene            18897..20300
                     /gene="glmU"
                     /locus_tag="PLM30_00220"
     CDS             18897..20300
                     /gene="glmU"
                     /locus_tag="PLM30_00220"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOR53624.1"
                     /translation="MIVMAVPGTGVLVLAAGKGTRMKSDTPKVLLELLDEPLLFYPMN
                     STDDPGFSGRAVVVGYGSEAVSRYLEEAWPGTEMIVQREQLGTGHAVFTAREWVSRFE
                     RVIIIPGDVPLMAKETLTRLNTRNIEADEDCTFLVFEPSDPTGYGRVLDTDRGFRIIE
                     EKDALEHEKKCCRVNSGVYVFRSGPLLEQLENIGRENSQGEYYLTDVIHRLCEGEKRV
                     GIMTCPDPDEMEGVNDPLQLDRVSRILRGRILDRHMRNGLKCVDSQTVWIGPGVVLGD
                     DVTVEPFVQVWGASKIGDRSRLGSFSIVRNSVLGENVHLLGHVVISDSVAKDDSTLGP
                     FTFVRDGSVLGEGAFAGKFVEIKKSIIGERSKIPHLSYIGDATVGKGSNIGAGTVTCN
                     FDGKKKNPTTIGEDCFVGSDTMLVAPVSLGDGCYTAAGSVITKDVPAESLAIGRARQK
                     NIEGWALKRKKTDGGGE"
     gene            20303..21271
                     /locus_tag="PLM30_00225"
     CDS             20303..21271
                     /locus_tag="PLM30_00225"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOR53625.1"
                     /translation="MASKLRELKVFSGGAHPEFTRSICENLGVGVATANIFRFSDGEI
                     GLSVEESVRGADVYVIQPTCEPVNENLMELLIMIDAMRRASAYRVNVVTPYFGYARQD
                     RKSKARDPITAKLVANLIEKAGADRVLAADLHAGQLQGFFDIPVDHLTGIPLLAAYFR
                     DALKKELEQDSLVVIPPDIGAVTRARRFAVPLNADLAIVDKRRSHEVANFSEVMEVIG
                     DVSGKTAVLVDDIIDTAGTMVQAAEAVLARGAKKVYACATHGVLSGPAIERISRSPIH
                     QVVLTNTIPLQEEKRIDKITVLSIAPLFAEAISRIHSDHSVSILFR"
     gene            21317..21976
                     /locus_tag="PLM30_00230"
     CDS             21317..21976
                     /locus_tag="PLM30_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOR53626.1"
                     /translation="MDKSTRIRLEKREETGKQHCKKVRNEGKIPGVLYGPGYLGSLPF
                     YVAVQSIAPIVQSGRWETARLDVETPGGKKELCLMRDLQRNPLTGSILHVDLLQLKKG
                     HKVSVNVPVEITGREKCAGVKQGGVFEQLIHEVEMEVLPSEIPASIAIDVTDLSLDGA
                     IRIADVKFPESAVLTLSHEEVIVTIAQPRVEVEAASEAAEEETTEVEVVTKGKAKEEE
                     V"
     gene            22016..>22611
                     /gene="pth"
                     /locus_tag="PLM30_00235"
     CDS             22016..>22611
                     /gene="pth"
                     /locus_tag="PLM30_00235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR53627.1"
                     /translation="MRYAWTRHNAGWHVLDLLRQRLGAPKSSLSLDSHAWGPIPVDGE
                     RVLLLKPMTYMNLSGSAVMKAFHFFDIGLEDLLVIYDDAALPFGRIRLRARGSAGGHK
                     GMVSIIAHLGSLDFPRLRIGIDSSGPQKDLAEYVTEVFSPEELAELPAVVERAADVAL
                     VWITRGIDEAMREANSAPPDTSGSSKGGFEKKGQPTLR"
BASE COUNT         4594 a         6445 c         7257 g         4215 t
ORIGIN      
        1 agtgggaccg tgtgaccgat cccgtccggg tggccagggt gaccatgatc ctttcgaggc
       61 taaccccctt caccgaacgg gacctcccct tcgaggtccg tctcgtggag gaaaagagac
      121 cgaacgcctt tgccctcccc ggcgggagga tatacatgac cacggggata cttgagttct
      181 gccgcagcga cgatgaggta gcctcgatcc tggcccatga actggtccac accgaccgga
      241 aacacggaat gatccagtca tcaaggaacc agcgcctgac ccttggtgcc ctggcggtga
      301 tcatcgccac ggggggttcc ggcgcggcgc cgatcctggc caacctggcg caggtggcga
      361 tcatgaactc ctacagcaag gacctcgaga gagaggccga tacggagggg ctgaatatcc
      421 tgtcgcagtc gggatacgag ccggccgggg ccgtgacggt catggagagg ttgcaggagg
      481 aagagatgaa gaggccctat gtcgatcccg gtatatacgc ggaccacccc aagaccaagg
      541 atagggtgag gagcctggag gacatcatca gggaaaaggg atggcccctg gagaggaaga
      601 aggcccttcg ccttttaagg gtgcagacga gcgaagaggg cggctccgtc ctgctcagca
      661 tagatgggaa gcccctatgg cgggcgccca aggtcgaggg tgccgctgaa ctgatgaaca
      721 aggcggccgg gtcgctggat aaatacctgc agatggaaac ctcgccctac gatatctcca
      781 tccaggagac ccacggccag aaagcgctca gggtgggcct cggggtgatc gtctatgaaa
      841 aagacctcct ccccggtatg ccctcgctgg aggaattcag ggagaacatc ctgctgtcgc
      901 tcaacagggc caggatggtc caccctgtgg ccgattacaa caggtaggcg aacccacggg
      961 gagcgccttt cacgggaggt gataggatgt acctttccct ttaccgccgg cacaggccgg
     1021 gggttttctc ggaagtcgtg gcccaggaag cggcggtcca ccttcttcgc agggagatcg
     1081 cgagcggtaa aaacagccac gcctatcttt tttccggccc tagggggagc ggcaagacca
     1141 ctctcgccag gatcgtcgcg aaggctgtga attgccccga ccgcgacgag gaaggggaac
     1201 cctgcggaca atgccccagc tgcgtttcca tcgcgtcggg ggacaacctg gatgtcattg
     1261 agatcgacgg ggcctcgaac aggggcatcg acgagataag ggaactcaag gagcatatct
     1321 ccctttcgcc cttttccgga aggttcaagg tctatatcat cgatgaagtg cacatgctca
     1381 ccgaaccggc cttcaacgcg cttctgaaga ccctggaaga acccccccgg acggtggtct
     1441 ttatcctcgc cacgacggaa ccgcacaagg tcccggcgac gatacggtcc cggtgccagc
     1501 acattccttt tcaccgcatc ccccttcccg ccatagtgga gaccatcagg aaggtgtcgg
     1561 cctcggaagg ggtgcatatc gaagagaaag ccctatggga gatagccagg gagtccgacg
     1621 gttcattgag ggacgccctt tccatgctgg agcaggcggt gaccacgggt ctcccatcgg
     1681 tctcgctcga ggaggtcagg ggcatcctcg gggggggcgg aaggagcgac ctggagaggc
     1741 tcctccccgc ccttagggat gggtcccccg aggcgttcag gggcttttcg gagcttttcc
     1801 agcgcggcct gacggcggaa cgcttcctgg agggcctgtt cctgctcttc agggatatcc
     1861 ttgtcgctgt cttgtggaag gaagagggga tcgattccct gccccttaac gaggaggaga
     1921 ggtccttcgt ccggagcgaa gccgggcact ggagagaacc cgacctctgg aaggcccttg
     1981 atctctgctc ggccaccctc cccagggcgc ggtatggcct caagggggat gtcgtcctgg
     2041 gcatgctcct cgggcttttc ctggcgcctg ggtcgaaaag agaacgtatc gaacggaaaa
     2101 aggaggagcc cctgtccgag ggagagttcc ctgtgaagaa ggcggcaggg ccggaagcga
     2161 tggggacggt cgaagggtcc cttcccccgg tgaagatcga aacccccgaa gatactccaa
     2221 ccccgaccag ctccacgggc gcggggggtt ggggagattg cgtcgcggcg gtggccgccg
     2281 ataaccttcc cctctattgc gcctgcgctt ccagcaattg cagaaaggac ggggattcgc
     2341 tggtggtggt cttccccgag gaacagcgct tctcctttga actggccacc tcccccagga
     2401 acctggcata cctggactcg ataaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnngat ggtttttact accgccccag ggtcgatcac gatctactct cccgttattc
     2581 caggggtctt atcgccagtt cggcctgtct cgccggcgag ataccgagcc tcatcctcga
     2641 gggctccgag gaggaagcgc tacagagggc catgctctac cgcgatatct tcggagaagg
     2701 caacttcttc ctcgagataa tgcacaacgg gatccccgag caggccgtcg ctaacagggc
     2761 gttgatcaag atgtcctccc agacaggcat ccccctggtt gccaccaacg acgcccacta
     2821 cctggaggcg aaggatgcca cctggcatga cgttctgctc tgcgtccaga cgaacgccac
     2881 ggtgaatgcc cccagccgat tacgcttcgg tgccgacgat ttctacctcc gctcgcccga
     2941 agagatgtgg tccctcttcg gcaacgacgt cccccaggct cttgaaaaca cggtggctat
     3001 cgccgagcgt tgcaacgtgg gtttttcctt cggttcctac cggttgccca gggtcgaggt
     3061 ccggaaaggg tccagcctcg aggaggaact cagcataaag gcccgcgagg ggctgcaggc
     3121 ccgtttcagc ggtgatgtag tgcccgcggg atatggtgag agactcgact acgagctcca
     3181 ggtcataaac cagatggggt tcgccggtta cttccttatc gtatccgaca taatccacac
     3241 cgccaaggag gaggggatcc ccgtgggtcc cggcaggggt tccgcggctg ggtccctggt
     3301 cgcctattcc ctgaggatca ccgagatcga tcctctcagg tacggcttgc tctttgagag
     3361 gttcctcaac ccggaaagga taagcatgcc cgatattgac accgatatat ccgaccgggg
     3421 cagggaccgg gtcctcgaga tcatcgtgga aaagtacggg cgctcccacg tggcgcagat
     3481 aatcaccttt gaccgtatga agagcagggc ggccatacgc gatgtgggga gagcgctgga
     3541 catgaagtat cccgatgtgg acaggatagc caagttggtg ccctcggggg ccgcctccat
     3601 cgaagaggcc cttcagcagt cccccgacct gaagcaggcc aggaaggagg acccttcggt
     3661 gagcttgctg ctggactatg cagcaaagat cgaggggttg gcccggcact gctcacagca
     3721 cgcggcgggc atcgtgataa caccggagcc gctgacggac gtggttccca tccgaaggat
     3781 cggggaagag cagatcgtga cccagtactc catggaaccc ctggagagcc tgggtctcgt
     3841 gaagatggat ttcctggggc tccggacgct ttcgatgatc gaagaggctc tctcgaacat
     3901 caggcggaac gggaaggaag cgccggacct ttattccatc ccttccggcg acagggctgc
     3961 cttcgacctg ctctggcgtg ccgacaccct gggcatcttc caactggaat cttcgggcat
     4021 gaggcagcta ctcaagcagg tgcgccccga ctgtttcgag gacctcatcg ccgctctcgc
     4081 cctgtatcgc cccggccctc tgggcagcgg tatggtagag cagtatatcg actgcaagca
     4141 tggccggaga gagatatcct ggcttcatga cgacctgaag gaagtcctca gggagactca
     4201 cggcatgttc ctctaccagg aacaggtcat gcagtgcgcg gcaacactcg cggggtatac
     4261 tctcggggag gctgacctgc tccggagggc catggggaag aagcaggccg acgtcatgga
     4321 gacccagcgg aagcagttcg tggaagggtg cgccggtaac ggtgtcaagg cttccgacgc
     4381 ggagcacatc ttcgacataa tccaggagtt cgccggctac ggtttcaaca agtcccacag
     4441 cgcggcctac gcgatgatca cctaccagac ggcctacctg aaggcccatt acccctgtga
     4501 gttctacgcg tccttccttt cgagccatat ccactcgaag ctggacgtgc tcgctaacca
     4561 catcaggacc gtcaggaact ccggaataaa ggtcctcccc cccgatgtca accggtcgga
     4621 ggccaacttc agcgtcgacc gggacgttat ccttttcggg ctgggcgccg tctccaagat
     4681 gggacagccg acggtggagc agataatcgg ggagcgcgag gcaggtccct acctatctct
     4741 ctgggatttc ataaaccggg ttgacacgag ggtcgttggg aggtcggtca tggagaacct
     4801 gatcagggcc ggcgcctttg actcgatcac ccccaacagg cggcaactta tggaatccct
     4861 gccgggcatg ttcgaactgg cccagaggag gaatgacgac tgccacagcc agcaattgct
     4921 ggagcttgag gacctgtccg aggaggaggg ccccgtcctc ttcgaagtgg aggattatga
     4981 cctccacacg aggatggaga tggaaaagga agccacgggg ctttatattt cagggcaccc
     5041 ctttgacaag tacgaaaagg aagtgttgcc ctataccacc tgctccatta ccgacctgtc
     5101 cttctggagg tccgaagact gcaccgcggt ggtcgccggg atactggcgg gggtccagga
     5161 gaagttcacc aggaaaggcg accgcatggg catcctcgag ctggaagatt cttccggtaa
     5221 ggtagaggtg gtctgcttcc ccaggacctg gtcgtcgatc acacctcccg aaaaggggaa
     5281 ggccgtcgtt gtgaaggggt cgcccaggac cagggacggc ctgtcgctga tcgccgagaa
     5341 agtcatggcc gtcgatgaac tggacggaag cacaagaaaa tggctccgcc tgagggtcct
     5401 cgatgacggc gcctcctcga acgggaggct cagggatatc tacagggaac tcaaggggca
     5461 ccccggtgag gctggagtcc tgctggaggt ccccctggaa ggggttaagg ccctcctgaa
     5521 ggtcagggac gtctttgtcg agccctcggc agatctttcc acccggatct ccgagttatc
     5581 gggcggctgc gtcgaagtgg tataaaattg tgaggtcgtc ccctcgagag gggcaccggg
     5641 agggaggagc atgcccgtgg agaaggaaga caagccttcg gtgctttctg atttcgttgt
     5701 ggtaaaggcc ctggaaaacg gggtctccgt gataggcatg acaagggggc aggagacgaa
     5761 gttcgcgcac tccgaaaagc tggatgccgg cgaggtgtgg atcgcccagt tcacggagtt
     5821 cacctcggcc atgaaaatca ggggcagggc ccacatactc acggcccacg gggtcgtcga
     5881 aagcgggagg aagaatgagg aaacgggcta agatcgtctg taccctcggg ccggcctgcg
     5941 aggatcatga agtgctgaaa agaatggttc aggcgggcat ggacgtggcc cgcctgaatt
     6001 ttagccatgg ggaccacgga acccacgggt cccacctgga caaggtgagg aggatcgagg
     6061 aggaactggg gcggcccatc ggcactatga tagacaccaa ggggcccgag atcaggaccg
     6121 ggaccctcga gggacaccgc ccgatgatga taaaaagcgg gagcacgata acgctgaccg
     6181 ccgaacccct tgagaagggt gacgcctcca gggtcggcat aagctacccc ggcctccccg
     6241 gcgaggtcgg ccctgggcag gatcttttta tcgacgacgg cacccttcat ctcatcgtgg
     6301 aaaaggtcga ggggaaggat atcacctgcc gtgtgaacgt gggcggtgaa ctgggcgaaa
     6361 agaagggcct ctccatcccg ggcgtgaaaa tgtcgctgcc gatcctcacg cccaaagaca
     6421 tagaggatat ccgctgggcc gtggagaaag ggatggactt cctggcgctc tcctttgttc
     6481 gcgatcggtc agacgtcatg gacgcgcgcc gggtgctgga agagatcggg ggcgacctca
     6541 gggtgattgc aaagatcgag acccgcgacg cggtggacaa cctggaggag atcatcgagg
     6601 tcgtggacgg gatgatggtg gccaggggcg acctgggggt tgagatcccc ctcgaggatg
     6661 tccccctaac gcagaaggcg atcatcgacc ggtgccgctc tcacggcaag cccgtcatcg
     6721 tggccaccca gatgctggat tccatgataa ggaatgtgcg tgctacgagg gccgaagcga
     6781 gcgatgtcgc caatgccgtc ctggacggtg ccgatgccct tatgctatcg ggcgaaacgg
     6841 ccaacggggc ttacccggtg ctggcggtga aaaccatggc cgggatcatc ggcagcactg
     6901 aaaaaggggg atccccctgg aggaaaccct tcatcttcca gacgggtgcg ccggggatcc
     6961 ccgacgcggt cagcaaggcc gcggggatga tatcggacca gatgaaggcc caggcgatcc
     7021 tttcactaac ccaaagcggc gtgacagccc agatggtcag caagtacagg cccgcggccc
     7081 ccattatcgg ggcgaccccc aaccgggaga ccttgaggta cttgaccctg gtctggggag
     7141 tggagcctct ccttgtcccc gaggagaagg tcctcgagac ggccctgacg tcggccatgg
     7201 agaccgcccg taaggagtcg ctcctgaggg agggcgatat ggtggtggcc accgccgggt
     7261 accctatagg cagcccgggg agcacgaact caatccaggt cttgaccgtg gccaagactc
     7321 tcctgaaggg actctcccta ctgaagaagg acgtctccgg ggtggttgtg aaggccctga
     7381 ccggctccga ggccgacgcc aagatgaagg acggggcgat actggtgacg aggcagaccg
     7441 ataaggaata cataccggcc atgagaaagg cctcggccat aatatgcgaa gagggcggcc
     7501 tcacgagcca tgccgccatc gtcagcctcg agctcaggat accctgcatc gtctccgcca
     7561 cgggagccct ggccgccctg gaggacggca tgacggtgac cgtggatggg agacgcggcg
     7621 tagtgctcgg aggcgcggtc cggctccatt ccgatgggga ctgagcggag aggggcctca
     7681 tggctccgac gggccctttt gaaaacaccc tcccaccggc tggacatagg gtgggaggat
     7741 ataaccgaaa gccaggggag gatgagcgcc gtcctggtgc ctctcttccc gaggggggag
     7801 gggctttttc tgctactggt ggagcggtcc gattccctga agcgtcaccc gggacaggtg
     7861 gctttccccg gcggtgcccg ggaaaaacga gacaggggcc ccgtcgagac cgccctgaga
     7921 gaattcaccg aggagacggg gatccccgcg gaaagggtgg aggtcgtagg cgcgcttccc
     7981 gaggtctggg tctattccag cgacttcacg atcttcccgg tggtggggta cctgccctcg
     8041 ggggtggccc ccggagacct gtcgcccgac gggaacgagg tcaagagggt gctggaggtc
     8101 cccctgaagc agctggaacg accaccgagg atggaaaacc ccgccggtga ggggcaaaac
     8161 ttcccctttc ctgtttaccg gctcgatgac ggggttattc tgtggggtgc ttcggctcgg
     8221 atcatcctcg accttacccg gtcactttgc gactcaaagg gcgggacacg atgtccctga
     8281 taggcgccca tgtctccgtc gccggggggc tctggaaggc catagaaaga ggggaaagtc
     8341 tgggctgcga ggccatccag atattcacca agaaccagct tcaatggctg accccacccg
     8401 tatcggagtc cgtggcttcg aggttcatgg ccgcctgggg caggagtacc atcaagaagg
     8461 tagtggccca tgcctcctac cttttgaacc tggcttcaga cacggagaag cgcgaaaaga
     8521 gcatcgccgc cctcgtcgaa gaggtggccc gctgcgaggc gctggggatc gaggacctgg
     8581 tccttcatcc cggttctcac cttgggcggg gtctggagta tggcaggcaa cgggcggccg
     8641 agggcctggc gcgcgccctc gagcggaccg ccgggatgag gacgagggtc ctcctggaga
     8701 ccatgtccgg ccagggacat tccgtcggct cgacaataga ggacctggcg tggatacttg
     8761 aaagggtggg cgacccttcc cgcgtcgggg tctgcctcga cacgtgccac cttttcgccg
     8821 gcgggtacga actgcggggc cggtcctcct acgaccgctt cgtccgggcg gtggagaagc
     8881 atatcggtct ggtgaacgta gggtgctggc atttgaacga ttccaccgcc gagatgggga
     8941 gccacataga caggcatcaa cacctcgggg aaggcttcat agggctcgag cttttttcgc
     9001 gcatcgtcgc cgacccccgg tgggaagaca tcccctgttt gctggagacc cccaagggcc
     9061 ttcccggaga cagcggtaac ctggcccttt tgagaaaatt gaggggccgt tgagtgagaa
     9121 tatagttgga atgatcgtcg tgaaggggag gcggtcccgt tgacttcgag gtacccggag
     9181 atgagcgtcg acacggccat gatcacaagg aacgccgaaa aggtggtcgg cctctgctcg
     9241 ggaaagggca tatcggtatg gggggtcaca aagggcttga gcgcccaccc ggcggtagca
     9301 tcggccatgc tggacggcgg ctgcgtgggg cttgccgaca gccgcgttga caatatcagg
     9361 aaactccgcc ttttcgggtt tcggcgcccc atgatgctgc tgaggatacc catgcccggc
     9421 gaaatgacca atgtggccga aaatgtcgac tgctgccttg tctccatgcc ggcatcagtt
     9481 ctgatgctcg atcaggcctg cgatgaggcc gggagtgagg tcggcatcat tgtcatggtc
     9541 gacctggggg acctacgcga agggatctgg ccggacggcg tggaagagat ggccgacgcc
     9601 cttcttcaat cccgcaggac ccgttgcctg ggggtcggga gcaaccttgg gtgcctgtca
     9661 ggggtgctcc catcggtgac gaacctctcg ctgctggtca ggatcggcgg ggagctctcc
     9721 aacctcctcg gacatcccct ggaagtggtc tccgggggtg ccacatcctc cctgtccctg
     9781 gtcgaggacg gcacgatgcc cgaaggtgtc aaccaactga gggtggggga gggtatcctg
     9841 ctcgggaccg atgtcacggg aaacagggtg atcccctacc ttgaaaggga cgccataagg
     9901 atcaccgccg aggtagtgga ggtcaggaga aagccctcga tgcccatagg gaagacgggg
     9961 gccgacgctt tcgggaacat cccggttttc caggacaggg gtatgaggcg gcgggtcatc
    10021 gtcgccatgg gacggcagga cgtgaggccc gaagggctga cgcccgtcgc cccggggctc
    10081 gagatcatag gggcttccag tgatcatctg accctcgacg cggaggattt cgagggcccc
    10141 ctcaacccgg gcgataccat ttccttcgct gccgattacg gcgccatgct ggcggcggcc
    10201 acctcaccct atgtcagaac gaagataata aagaaatgac cgccggagag tgatcccttg
    10261 tccggaatac tcgaatccat ccgttggcaa gactttattg acatcatcgt catagccttt
    10321 atagtctaca ggctgctcct cctgctcatg gacacccgcg ccatgcagct cgtgaagggc
    10381 cttttcgtca tcggcctttt ctacgccacg gcccggttcc tgaaactggc ggctctgacc
    10441 tggatactcg gaaggcttct gggggtcttc atcctggcga tccccatagt attccagccc
    10501 gagatcagga ggatgctcga agaactcggg cgggggagca tctggagacg ccgccaggcc
    10561 gaggagaggg cgtcccagtt gaccgacgag atcaccaggg ccctcctttt tctccagagc
    10621 aggaagatag gggccatttt ggtctttcag aaagacaccg gcctgaagga catctggaga
    10681 tcggccgtcc agatcgaggg ggatgtatcc caggacctca tcatatccat attctggccc
    10741 gggaacccgc tccacgacgg cgcggtgata ctggacgacc ggaaggtgat cgccgcggcc
    10801 tgctacttgc ccctcacgga gaacagcaac ctctcaagat ggatcggaac ccggcatcgg
    10861 gccgcccttg gcgtttcgga ggtctccgac gccatggccc ttgtcgtatc ggaggagcgg
    10921 ggggaagtgg ccttggccat caacggtcat ctttcgaggg aattgaagga agggcagatc
    10981 aggaggctcc ttttgcacta cttcacccgg gccgccggtc ccgcgacagc cgtcgagggt
    11041 atcagggaaa ggctggggtc tgtgttttcc cggggaggat ctgacaatga tgagatcgat
    11101 tgatcgcctt ctcgcctccc ggctcttcct caaggtattg tcggtgctgg tagcggtcct
    11161 ggtctggttc tacctggcct cggaccgtgg gaccgaggtc gtgaggaccg tgacggttcc
    11221 gctggagttc ctcaacgtcc cggccgatat gtcagtgacc agcggggtcc gcgacgtgga
    11281 catccaggta tcgggcacta gggaggatac cctcctgaag atggacacga tcgcctcgca
    11341 ggtggacctt aagggcttag gaccgggatc gtaccgccga cccatccagg ccatcctttc
    11401 atcggggctt aggctcgtcg aggtttcccc gcccttcgtg gatctcgacc tcacccggtt
    11461 agtgtcgagg accctgaccg tcaaggtcct cgtccccgag gacttgccgc ctgaatacca
    11521 tctggaggag gagagggtcg aaccccggga ggtctcggtc aagggtccag agcagatggt
    11581 ggcctccctg gacaacgtct gggtggctcc caccgttgaa cagctgcttc aggagaagga
    11641 gctctccctt tcggtcatac cctttcccgg cagcgaccag aggtcgccct tcatgataga
    11701 accggccgtg gcggacctgt ttttccggtt gatcaagggg ttccccagga aggtcgtccc
    11761 tgtgagggtt gaactgaagg gtgaaccggg tcatgacttc caggtggaag cggtggtggt
    11821 ggacccaccg gaactgacga tccagggtcc ctttgagtcg atagaacgaa tataccaggt
    11881 caacttggcc cccatcgata tcaccgggct cgcttctgat gtcagcgagg tcgtgaccgc
    11941 gccggacccc gccgatgacg cgcagatcgt ggatgtgccg tcggtccggg tgaggatcct
    12001 tctggctcca aggatggaaa gaaggctttt cgcgaagata cccgtccggc ttgccggcag
    12061 gagcatatac cccggctgga gggtggagcc ctccgaggtg aatatcattc ttcaaggccc
    12121 gccgtcggtc ttggattccg ccgaggccga aggaagcccc ctggacgtct tcgttgacgt
    12181 gacaaacctg gtctcccaga agatcaccgt acccgtgaag gtgaccttgc gtgccagggg
    12241 ggccaccctg gccgaggtcg acccacccaa cgttacggtc tacgcgttga cggagtaggg
    12301 tcttggaaag gagtgtaccg atgctggttc ccttgggaaa aagccgttgc ctcttcggta
    12361 ccgacggtat cagggatgtg gccaaccgag gtctcatgac cccggagatg gcgctgcggc
    12421 tcggccggtc ctacgccctt tttctcattg aaaggggggt atcccgcccg accatcgcca
    12481 tcgggaggga cacgaggcgt tccgggtaca tgatcgaaat ggccctggtc tcgggtctgc
    12541 tctcggccgg cgccgaggtg gcctccctgg gtgtgatccc gacccccggc gtgagcttcg
    12601 ccgtcaagag gctttcctgc gacggtggag ccgtggtgag cgcctcccac aatcccttcg
    12661 aatacaacgg tataaagttc ctgggcaagg atgggggaaa gctgagcgac gaagaggagg
    12721 ctgagatcga ggattacctg gccgatgtcg ttttcgatga gtggaggccc accgggtcgt
    12781 cgataggttc ggtctcaccc aggcctgaga ttctccatga gtatacggag aatctcgtaa
    12841 ggacctgttg ccttggtgcg gatccggagc tctccatcgt ggtcgactgc gcccacgggg
    12901 ccgcctcgga ggctgccgcc ctggtcttcg gaaggtgttt caagaaggcc gcccttatct
    12961 ccgataggcc tgacaggtac aacatcaatg atggctgcgg cgttatccat atcgataatg
    13021 tggccaccag tgtcctttcc gagggctatt ccctggggat ggcccttgac ggcgatgccg
    13081 acagggtcct tttcgtcgat cccaggggca gggtgatcga cggcgacatc atcctctgga
    13141 tcctcgggag atggctcctt tccgaaggaa agcttggcaa cggtgtcgtc gcgacggtaa
    13201 tgagcaacaa ggccctggag gagaagctct ctcaggaagg gataacgcta cagcgctgcc
    13261 ccgtcgggga caggtacgtg ctggacgcga tgaggttgtc ggggtcgaac ctcggcggcg
    13321 aacagtcggg ccatatcatc gccggcgact tcttccccac cggtgacggc ctgggttccg
    13381 gactcctttt cctctcggcc tgcgcccgac ttggagagga tacggacacc ctggtcgacc
    13441 ggttcgaaag gtacccccag gtgcttaaga attttcatac ccgtgacaag gatgcctttc
    13501 tcggccactc cgaagtgcgg gaggccatca gcaaagcctc cttcgacctg ggggacctgg
    13561 gtagaatact ggtcaggccc tccggcacgg aaccgctcgt gcgcgtcctt gttgaggcca
    13621 gggatgacac tttactagaa gcggtttcgg gtacgctctt tgaaagaata gcgaaggtcg
    13681 cctctagcgc cgctttcggc agcctcggag gaggtgaaga gccttgatgg gggaccgggg
    13741 cagaaaatgt ctttttcccg tggcgggaat gggcacgagg ttcctccccg ccacgaagga
    13801 gatacccaag gagatgcttc ccctcatcga caagcccatc atccagtacg gggtcgaaga
    13861 ggccgtccgg tcgggctgcg tcgagatcgt cttcgtcacc ggcagaggta aaagagccat
    13921 tgaggatcac tttgacaggt ccatcgaact cgagcagctg ctcctcaaaa agggcaaggc
    13981 cgactgcgcc cggatcgtga gggagataag cgagatggcc gatttcgcct atgtgaggca
    14041 gtccgaaccc ctcgggctgg ggcacgctgt cctctgcggc gaacccttct gcagaggaga
    14101 gcctttcggg gtcatcctcc ccgacgacgt aatgatcagc ccgccgggct cgccgcccgt
    14161 gctcgaacag cttttcctgg ttcacgagag gctgggaggt tccgtggtcg ccctcgagga
    14221 ggtccccctg gaggaggcag gcaggtacgg gatgatcaaa ggtgaaccgg ttgaaaaggg
    14281 ccttttcagg atagaggacc tggtggaaaa acccaggccc ggcggggtac caagccgtta
    14341 cgcggtgatg ggccgttacg tcctttcgcc gaagattttc cgtcacctga agagttccgc
    14401 cgccggggcc ggggatgaga tacagctcac cgacgcccta aaagtcctgg cgaaggatga
    14461 gcccctttgg ggggtcctgt acgagggcga gaggtttgac tgcgggacca tggagaactg
    14521 gctgaaagcc acgctgggca tggccctggg gcgggaggat ctgcgggaca tcgtcctttg
    14581 tgaactcaag cggctcggtg tcctggaagg aggtgatggg gcggcggtga gtgaagatgg
    14641 gaacgtgcgg tcatcctagc gccagaactg gcctggccag ttgacgaggt ccggggagca
    14701 tcgaaaattc ggcggatgcc ccggtggcac cgacggaggg gccatgaagc cgcgccacaa
    14761 aaccggggag cgatccccgg gacaaaggga gcggcatccg ttttttttgt gcgattgaaa
    14821 ggcggtgccc agacagtaat gtgcgggatc gttggttatg tcggcggacg ggaggcccga
    14881 gaagtcctta tcgatgggct gaagaggctt gagtatcgag gctacgactc ggcgggcctt
    14941 gccctggggt cgggcaagga cctcctcatc atcaaggagg tcggcaaggt ggaagacctc
    15001 gagaggatgc ttcaaaaggt ccccgcgggt tcccgccagg ggatcggcca cacgcgctgg
    15061 gccacccatg gcggggtctc ccaggccaat gcccaccccc ataaggacgg cagcggccgg
    15121 tttgccctgg tgcacaacgg gatcatcgaa aatttttcgg aactccggga agaactcgaa
    15181 agggaaggcg tggtcttcct ctccgagacg gacacggagg ctatcgtcca tctcgtcgca
    15241 gcgtccttct cccggacggg tgacatcctg gaaacgctgg tataccttca gaagaggctg
    15301 agggggacct tcgccctggc gctgatgcag cagggtttcc ccgacaggat ctacaccatc
    15361 cggagaggct cccccctcgt cctgggatcg ggtgatgggg aggctttctg cgcttcggac
    15421 attccggcct tcctgcccta cacgaggagg atccagtacc cggaggaggg cgatatagcg
    15481 gagctctccc cgggcggtat caggatatgg gatgagaacg gccgtcccgt ggagaggccc
    15541 gccgttaccg tcgactggga cgtgtcgatg acagaaaagt cgggtttttc ccattacatg
    15601 ctcaaggaga tccacgagca gggaacggta ctccgccgtt ccctgaagga cagggtcgtc
    15661 gacgggaaaa cgatgatatc gggggaactg ggctgggacg atgagatggt gtccaaatgg
    15721 aagaagatcc atatcgtcgc ctgcgggtcc tcctattact cagccctggt ggccgaaagg
    15781 ctcctggaaa agtggaccga cctcgagatc aaggtggata tagcctcgga gtacaggtac
    15841 cgccatgtcg cctgcgatcc ctcaacgctg gccgttttcg tctcccaatc cggcgagacg
    15901 gcggatacgc tggcggcaca gcgcaaggcg agggccggcg gggcgcactg cctggccata
    15961 accaacgtga gaggttccac cctggccagg gaggttcaca gcgtgttgct cctcaaggcc
    16021 ggcccggaga taggcgtggc ggctaccaag agcttcaccg gccagttggc tgcccttttc
    16081 ctggtggccc ttcaactggg gagcatgaga gggagcctgg ctccccacga agaagaaagg
    16141 ttggtcaatg aactggtccg gatcccctac aaggtggaac aactcctgga gaaggaaggt
    16201 gagatcaagg aggtcgcgag gaaatacgca cggaataaga acatgctttt catcggcagg
    16261 gggatgtcct tccccatcgc gcaggagggc gccctcaagc tgaaggaggt ttcctacatt
    16321 catgccgagg ctctcgccgc gggagagatg aagcacggcc ccatcgccct gctggagccg
    16381 ggggtgccgg tcctggcggt catacccaag gatagccttt acgaaaagac cctctcgaac
    16441 atacaggagg tcaaggcgag gaatgcgtcg ctgatagcag tgtgcaccga gggggaccgt
    16501 tttgtccccg aacacgccgg ttcggtcata gagaccccat ggaccgacga ggaattcacc
    16561 cctttcctct cggtaatacc cctgcagtta ctgtcttttc acatagcgtt gatcctcggg
    16621 agggagatcg accagccccg caacctggct aagagcgtca ccgtagagta aaaggcgtca
    16681 ctggagctgg tgggacgggt cggtcccgtc ccgcagcggt tccctggtga aggggtagta
    16741 gatcctcatg tgagtgatgt cgttgagagc gtggtcctgc ctgccggaaa gagccggcat
    16801 cttcctccag ggtcctccct tcaacctggc gaggacaagg ggtttacgcc cgtaactaga
    16861 ccacaggtcc cacagcacct cacccctggc cggcccgggc aggagtcccc cggaaaacag
    16921 ggggcccgac cctttcaggt cgaccttccc catctcgggg ccgatgatca tccattgggt
    16981 catcttccat gccccgagca tctcgcggga aaaagcgtgc tccagcggga tgatctggaa
    17041 acctcccacc aacccctccg gcgaggtgca gacatctatg acgccggggt ggttcgccct
    17101 tattctgccc gtatcctgaa agattgtccc gtcgaaacgc cccgtaccgc cgaggggtct
    17161 tatcaccctt ccgagcaccc tcaccccgtc gccgtaccat gccgtcacac gcccccccgg
    17221 gcggttctcc aactcaacga agtaggggca cgggagagga tcggtcatga tcttcatgac
    17281 gtcccctttt cgaggtacgt cttccaccag gtagggatcg tcgtcgctat gctcgaagac
    17341 gaccctgctg ccgaccggcg gggcccaggc cccgaataac gcgcgccccg ccttgatgtc
    17401 ggtcacgatc gccgcaccag ggccggcggc gggagcgatg gtctcctcgg gcaaaaggct
    17461 tatggtcctc ccctttccct tttcaaccga caggaggata tggatggcgt tcaccgcact
    17521 cgccaccaca ctctgctccc tcccccaggc tgacgcggtg aaactgggcc accggctgtt
    17581 ggcggggagg gctttcaccc tccccagcac tgttttttgg ccgtcttctc tttggacggt
    17641 gatctccccc ccgggagtga gttcgacggt tatcgtgaag gaaccatcca gaacgccggt
    17701 ggaagggggg agggccgcag cggaggcaac ggccggcgga aggtccgcgg ccaggaagag
    17761 gagcaagagc attgacaaga aattcattat cgatttccgg gacgctctcc tcctcattgt
    17821 ctcacctgtt ccttgagggg tcgttccagc cggccttctt ggggtaaaag taaaggagtg
    17881 cgaagatagc ggcgggcacc atgggggcca gggctatcca gaggggcgcg caaaagaaaa
    17941 gggtgaggga tgaaaaaaga aggaaaaata aaagcgatcc ccagaaggcc gatgggcgga
    18001 cccggcttcc gctcctggag accagcaccc ccaggagcac ggacgtgacg aagaagaaga
    18061 ctgtcagtgc cagggggatg cggaacatgg ttcagtgggc gcggggggcc ttccttggcc
    18121 tggcgccggt catttctcgc ccgctacagg cacggcaggt gccataacag taaacctgta
    18181 ccgcatcgag gttgtggggg accgatctcc ttatcctgtt caggatctcc ttttccagcg
    18241 gtatgtcctc cagggccccg cacttcgagc agaggaaatg tgcgtggggg gtcgggtcgg
    18301 ggtcgaagaa aaccctgcga tcgtcaatgg agagtgtctg gagaaggccc gcgtcacgga
    18361 gtatctgggc ggtgctgtag atggtggcaa aggaaatggt ggggtagatc tccttcatct
    18421 cgcggaagat cttctccgcc gaggggtggt ctttccggtc ctcgaggagc ttgagtatgg
    18481 cgatcctctg ggaggtgatc ttcaagcccc tgctcctgat acgttcgatc ccgtcctcga
    18541 cggtccacat gctccaagac ctccccttca ttatacggcc cttcggagtg acgcagccta
    18601 catgaagttt accatatcaa gacagtggcg acaccggacg ctccccctcc ttgacactct
    18661 gaccaccggt gaggctataa ttgcgaggtt cgacccttgg ggtgtagcca agtcggcaag
    18721 gcgacggact ttggatccgt gaccgctggt tcgaatccag ccaccccagc cagttggctg
    18781 gtccagggga agcctcggct tcccctgctt tgttgtgctt ttttggctga agtcggacca
    18841 gccgtgttaa aatcaagaag gaaaagttca aaaaggataa tgatcagggg gtgaggatga
    18901 tcgtcatggc tgtccccggc acaggggtcc tggtcctggc ggccggcaag ggtacgcgta
    18961 tgaagagcga cacccccaag gtccttctcg aactgctgga cgagccgctc ctcttttacc
    19021 ctatgaactc gacggacgac cctggcttct ccggcagggc ggtggtggtg ggatatggga
    19081 gcgaggccgt ttcccgatac ctcgaagagg cgtggccggg gacggaaatg atcgttcagc
    19141 gggaacagct cggtacgggc catgccgttt tcacggcccg cgagtgggtc tcgcggttcg
    19201 aacgggtgat catcatcccc ggggatgtcc ccctgatggc caaagagacg ctgacccggc
    19261 tcaacaccag gaatatcgag gccgatgagg actgtacctt tctggtgttc gagccctcgg
    19321 acccgacggg ttacgggcgc gtcctcgata cggacagggg tttcaggatc atagaagaga
    19381 aggacgccct tgagcacgaa aagaaatgct gcagggtcaa cagcggggtt tatgtcttcc
    19441 ggtcagggcc ccttctggag caactggaga atatcggcag ggagaactcc cagggggagt
    19501 attacctcac cgacgtcatc cacaggctct gtgaaggtga aaaaagggtc gggataatga
    19561 cctgtcccga ccccgatgag atggaaggcg tcaacgaccc cctgcagctg gacagggtct
    19621 caaggatcct gagggggcgt attctcgacc gtcatatgag aaatggactt aagtgcgtcg
    19681 attcccagac tgtgtggatc ggccccgggg tggtattagg tgatgatgta acggtcgaac
    19741 ccttcgtgca ggtttggggg gcatcgaaga taggcgatcg ctccaggcta ggcagtttct
    19801 ctattgtcag gaacagcgtt ttgggcgaaa atgtccacct gttgggtcac gtggtgatca
    19861 gcgacagtgt agcgaaggac gattccaccc ttggcccctt caccttcgtc agggacggtt
    19921 cggtgctggg cgagggggct ttcgccggca agttcgtgga gatcaaaaaa agcatcatcg
    19981 gggaaagatc caagatccct cacctgtcct acatcgggga tgccacggtg gggaaaggga
    20041 gcaacatagg ggccggcacc gtgacctgca actttgacgg gaaaaagaag aatcccacca
    20101 ccataggtga agattgcttt gtgggcagcg acaccatgct ggtggccccc gtctcgttgg
    20161 gcgacggttg ctacaccgcg gccggatcgg tcatcacgaa ggacgtcccc gccgaaagcc
    20221 tggccatcgg gagagccagg cagaagaata tcgaggggtg ggctctcaaa agaaagaaga
    20281 ccgacggggg aggagaatga agttggcctc aaaattgcgg gaactgaaag tattttcggg
    20341 cggcgcccat cctgaattca ccaggtcgat ctgcgagaac ctcggggtcg gggtggcgac
    20401 ggcgaacatc ttccgcttct ccgacgggga gatagggctt tcagtggagg agagcgtgag
    20461 aggtgccgac gtttacgtga tccagcccac ctgtgagccc gtcaacgaga atctcatgga
    20521 actcctgata atgatcgacg ccatgaggag ggcttcagct taccgggtga acgtggtcac
    20581 cccctacttc ggctatgccc gacaggaccg taaatccaag gcaagggatc ccataaccgc
    20641 taaactggtg gccaacctga tagaaaaggc aggtgccgac cgcgtcctgg ctgctgacct
    20701 gcacgccgga cagctccagg gtttcttcga catccccgtc gatcacctca cgggcatacc
    20761 cttgcttgcg gcctacttca gggatgcact caagaaggaa ctcgaacagg acagcctggt
    20821 ggtcatcccc ccggacatcg gtgccgtgac cagggccagg cgtttcgccg tccccctgaa
    20881 cgccgacctg gctatcgtcg ataagcgccg ttcccacgag gtggccaact tcagcgaggt
    20941 catggaggtg ataggggacg tctcgggcaa gaccgcggtg ctcgtcgatg acataattga
    21001 tacggccggc accatggtgc aggcggccga ggcggtcctt gcgaggggtg caaaaaaggt
    21061 gtacgcctgc gcgactcacg gtgtcctctc gggtcccgct attgaaagga ttagcaggtc
    21121 acccattcac caggtggtcc tgaccaacac gataccgctt caggaggaga aacggatcga
    21181 caagatcacc gttctctcca tagcgccgct tttcgcggag gccataagca ggatccattc
    21241 cgatcattcc gtcagcattc ttttccggta gatcgccgcg gcctcctgtt cttctatttc
    21301 acgaggaggg tatttcatgg acaaaagcac caggataagg cttgaaaaac gggaagaaac
    21361 ggggaaacag cactgtaaga aagtcaggaa cgaagggaag atccccggag tcctttacgg
    21421 gcccggttac ctggggtcgc tgccttttta cgtggcggta cagagcatag cccccatcgt
    21481 tcagtccggg cgctgggaga ctgccaggct tgacgtagag acccccgggg gtaaaaagga
    21541 actctgcctc atgagagacc tccagcggaa tccccttacg ggcagcatcc ttcacgtgga
    21601 tcttctgcag ctcaagaagg ggcacaaggt ttcggtcaac gtgcccgtag agataaccgg
    21661 gagagagaag tgtgctggcg tcaagcaggg gggcgttttc gaacagctca ttcatgaggt
    21721 cgagatggaa gtcctcccca gtgagatacc cgcctccatc gcgatcgacg tgacggacct
    21781 gtctctggat ggcgccattc gtatcgccga tgtcaagttc cctgaaagtg ccgtcctgac
    21841 cctgtctcat gaagaagtga tcgtgaccat cgctcagcct cgcgtcgagg ttgaagccgc
    21901 gtcggaagcg gcggaagaag aaaccaccga ggtagaagtg gtcacgaagg gcaaggcgaa
    21961 ggaagaggag gtctagcgct ctttgaaact tgtcgtgggg ctgggtaatc cgggagtgcg
    22021 ttacgcctgg acgaggcata acgccggttg gcatgtactt gaccttcttc gccaacgttt
    22081 gggggccccg aagtcttctc tatcactgga ctcccatgcc tggggtccca tcccagtcga
    22141 tggcgagaga gtgctacttc tgaagccgat gacctacatg aacctgagcg ggagtgccgt
    22201 catgaaggcc ttccacttct ttgatatcgg gctggaagac cttcttgtca tctacgatga
    22261 cgcggcgctc ccctttgggc ggatccggct gagagcgcgg gggtccgccg ggggccacaa
    22321 gggaatggtt tcgatcatag cccacctggg atcccttgac ttccccaggc tgcgcatcgg
    22381 gatcgactcc tcggggccgc aaaaagacct ggccgaatac gttactgaag tcttttcacc
    22441 cgaagagctc gccgagctgc cggccgtggt ggaacgggct gccgatgtgg cgttggtatg
    22501 gatcacccgt gggatcgatg aggccatgag ggaagccaac tcggcgccgc cggacacttc
    22561 cgggtcttcg aagggcggct ttgaaaagaa ggggcagccc accttacgcg a
//
LOCUS       DAOSRG010000003        21722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_2605_length_21722_cov_8.355656, whole genome shotgun sequence.
ACCESSION   DAOSRG010000003 DAOSRG010000000
VERSION     DAOSRG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 21722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21722
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2605_length_21722_cov_8.355656"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1662)
                     /locus_tag="PLM30_00240"
     CDS             complement(1..1662)
                     /locus_tag="PLM30_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR53628.1"
                     /translation="MLSELMVTNVGGIREASLHFRGNFIVITGESGAGKSSLVRALEL
                     VSGKRAQSSIIRAGVEEAEVRAALEMDAPLASLPEDLQPQEGFILVKRVVSSAGRAKT
                     YLQEKPVPLNTLSGALSKVIAIQSQFAQLELLEPDQQAELLDNSGGDPLRSIRSNLSQ
                     AVSLALGNERGLLEITRRRREIESRYQEGEAFLEKFSRMGIRPGCESEWETDMNRVSE
                     QLKKQGLLRLAHDRLTGGSSGEGLSSAIEQFCHEVRQSLNEIPELEGSIERLLSSTQE
                     IESGLKGVFSVEREQSLQEEQEKLEKRLGSLKKLMRYAKVDNAEDLVDFAERVHSELE
                     WLRDSRETLARLQERSASLKKEVSALALSLRGSRKEAAEKLEMAVNVSLQGLAMEGLR
                     FGVSLQPLEKVRQSGADSIAFYLTDDKFLEGPVSKIASGGELSRILLALQAAASDEML
                     PQTLVFDEVEAGLGGRSAFLAGQTLRDLSKRCQVILITHEASIASLADQHFRVEREGD
                     ISRVLEVEGKQRERELARMLAGDPDDPEAAGLARSLLEANRQELG"
     gene            complement(1667..2545)
                     /locus_tag="PLM30_00245"
     CDS             complement(1667..2545)
                     /locus_tag="PLM30_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOR53629.1"
                     /translation="MIKTLGLLVNTQKGAALALAESILRWGRERDVNFALPPHESHVL
                     GEQETSDQEWKERVDAAVVLGGDGTFLRAARYVIGHDIPLYGINVGRLGFLSCGKIES
                     AEEDLEKIMAGDYSINRRPLLHGIVSRGKRHKHEVYALNDLVVTKGPFARMIALDIAV
                     SGRPLNLLHADGIIVATPTGSTAYSLSAGGPIVPPHLSCMILTPICAHTLYSRPIVLS
                     ESDTVTITPAGQHREIMLTQDGQLGYELLPGDRISISLDQDRTIGIITLPENEFFTLL
                     QEKFQWGHRTPSILEE"
     gene            complement(2542..3402)
                     /locus_tag="PLM30_00250"
     CDS             complement(2542..3402)
                     /locus_tag="PLM30_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOR53630.1"
                     /translation="MKPSKGDERLDLELVKRGIVESRNRAQALVMAGNVRVNGEKLVR
                     PGAKVSPDAIIEISGECRWAGRGAYKLLKALDVFPVDPSGRICIDIGASTGGFTDVLL
                     DRGAKRVYCVDVGYGQLAWRLRNDERVVVMERTNARYLEPGHLRDVPEIATMDVSFIS
                     VTKILPAVEALLAPDGDCIALVKPQYEAGKGLVGKKGVVRDRRLHRSILISLSLFTRS
                     MDKMALMGADFSPILGPSGNIEFLFHLRKGGVPPAGLDEAFFGDLVEKAHRELVEVRD
                     KKASKGGSQG"
     gene            complement(3380..5248)
                     /gene="dxs"
                     /locus_tag="PLM30_00255"
     CDS             complement(3380..5248)
                     /gene="dxs"
                     /locus_tag="PLM30_00255"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR53631.1"
                     /translation="MKDLLGSLVDYRDLEGLDHPGLGLLAKEIREMIIKTVESNGGHL
                     ASSLGAVELNIALLRSFDPSKDRVIFDVGHQAYAYKILTGRKDRFKTLRKWKGISGFP
                     DPKESPFDHFNAGHSSKSLSAALGQAKARDLLGQDHHVVAVIGDGSLINGLALEALNH
                     VHELDTPVIFILNDNQMSISKRVGGMASHLAALSVNPMYRQFKRFLKYFCHRVPEGKK
                     LESLLERSKQTLKRFLLPPNIFEDLGISYWGPFDGHDIQEMEKIFSMAKGYGYPLLVH
                     LLTTKGKGFKPAEEDSEKFHGISPCPSALTPGEPSPGSWSEASAACIEELAGLDQRVV
                     LLTAAMKEGSKLGPFQKKYPERFFDVGIAEGHLLTFAAGMASAGLLPVVSIYSTFLQR
                     AMDQLVHDICMQDLHVILAIDRAGLVGEDGETHHGVLDLPWTRAIPNLNVMAPRDLED
                     LKWIYREALKNGSPLAIRLPRGAAPASICREGDTPRQVWGRAERILKGTGWCLFSYGA
                     TIPLALSVFEKAEKTGVEPPSVYDLRFLKPLDVDAVEEALRTHPLAVVLEDGNIEGGI
                     GEKISLFADRVRVASRVNCLGVPDRFIPHGTVREQWEHCGLVPEEVLKIYETFKGR"
     gene            complement(5275..6198)
                     /locus_tag="PLM30_00260"
     CDS             complement(5275..6198)
                     /locus_tag="PLM30_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR53632.1"
                     /translation="MATKGPSPNDDPEARISEGRLFIEKALLDLCEKNPPGIPQRLWD
                     SMTYSLLAGGKRIRPLLCVLSGEAFGVSREDLLPMALACEMIHTASLIHDDLPEMDDA
                     ALRRGKPSNHAVFGGSLALLAGDALFIWAFEFARAGLLASGAFSCHSILEALGVILDA
                     AGPFGICGGQVLDTDPLSVGSREEHPWTVARSKTAVLLSACVQAGAILGGAEPDERRL
                     FAVFGDHLGTAFQIADDIIDVTGQASKTGKIPGKDSTRGKITFVSVFGLEEAKRMAQE
                     ETSLAQKALSAIERDVAALERFPEMLAGRDR"
     gene            complement(6188..6427)
                     /locus_tag="PLM30_00265"
     CDS             complement(6188..6427)
                     /locus_tag="PLM30_00265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOR53633.1"
                     /translation="MSFTKDMAELQEIVSRLDSGQIPLEDSLELFERGIGLVNSCRKY
                     LEEAQQRITLLSAEQGESEGREWDPLSGDEPADGD"
     gene            6564..6758
                     /gene="rpmB"
                     /locus_tag="PLM30_00270"
     CDS             6564..6758
                     /gene="rpmB"
                     /locus_tag="PLM30_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR53634.1"
                     /translation="MANVCDCCGRGRGAGNRVSHSNRHSRRTWGINLQTVKVDLGGGE
                     SRRMKICAKCLRSGRVRRAV"
     gene            complement(6755..7516)
                     /locus_tag="PLM30_00275"
     CDS             complement(6755..7516)
                     /locus_tag="PLM30_00275"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOR53635.1"
                     /translation="MALIFLSQLKAQFTPPFEPPSIVFVAGGRTPEPSWLRELCREKD
                     VWAVDRGLDSCMAAGIRPSFFIGDNDSVSEEARQWSREEKIPSLLFPPEKDLTDLQLA
                     LKEVGGTCGGPNGTRGCTAILTGAFGGRFDHLFANLYSLIWAEEEWGVKVRCEADESE
                     ALFILKSGETLRLSGLEKGGIFSTLALSEKCLGVRMTGTKWTIGEGSLYLKKPFAVSN
                     VAEAAVEVGLSSGWLGIYWATREYAEREQKQKGRN"
     gene            complement(7532..9760)
                     /locus_tag="PLM30_00280"
     CDS             complement(7532..9760)
                     /locus_tag="PLM30_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOR53636.1"
                     /translation="MKVTRRKPKARDKTGSKSPGRKEDMKGGRKQPGVTMAVFAFLLV
                     DLYLLATLFLFRTGEFGEVIHSWTLEAFGGGIIVPLVFGAYLGASRVLGYPIPFPLKQ
                     TLASSILYLCCALFLGLSALSGIEPAVALLRPGLVGSFLAGILLRHIGPLGAVLAGFG
                     GIAAALTLYGIINKKSMTKAASFLSRVLRAFGNVLSPSPRERPEKSVSPSVEPRKGVS
                     RIDPRADAKKPGISPTASRGQIDIPDTIVDEDAPDQAGAFPKTPPGQGLVFPVPLDIF
                     GPPDDFSFSLDKRLLSEQGENIIETLDEFGIAAELAETVVGPTVIQYRIQLAPGIKVS
                     RVSSLANDLAVSLAVPSLRIEAPIPGKTFIGIEVPNPERKPVNIRTVLDHPSFQEADC
                     DLAIPVGVGVDGRHLTIGLEKMPHLLIAGTTGSGKSVFVSCCIAALACRMKPEELRFL
                     LIDPKRVEMKIFDRLPHNITPPVVESRQAVHALGWTIREMERRYEMFAQARVRNIRAF
                     NARALPLDRLPYIIIVIDELADLMFTSPREVEDYICRLAQMARATGIHLIIATQRPSV
                     NVVTGLIKANIPARVAFTLPSQTDSRTVIDISGAERLLGSGDMLFLTPRLPKPLRIQS
                     PWMDEPVMVRFMDYLSTLFGDPVFFDIDMEDTGETNGVKAHLDDPLLEDAMNLILQSG
                     IASASRLQRQLRIGFTRAARLIDTLEQVGIVGPQEGSKPRDILVDEEEARQILEASTN
                     NL"
     gene            complement(9762..11117)
                     /locus_tag="PLM30_00285"
     CDS             complement(9762..11117)
                     /locus_tag="PLM30_00285"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR53637.1"
                     /translation="MDKLFDVADALSIPGTSFGEAFIQRGLARFCLMDDGKFEEVSSS
                     ITEGSGIRLVVGDRTYFTHSSGTDLSSVALSLAEGKRRAGLSRGKRLPKGERPLLHLP
                     VMNEEAPFDRIRDLDRVVRKLSSSIRQATFAWSSGVGEVAIAGSANPLCRRSDFSTRF
                     SVNIVVEADGSLFSGRAAKAFSLPQDEFLKIVDLEDVAREAFRRALLLSQAKPCPASA
                     MPVVLAGKAGGTMVHEACGHSLEADIVLKDHSSFRGSLGKRVASSLVTLADDPTLFGL
                     YGSYPFDDEGTPSRRTALIKEGVLVSFLTDLLTSKEGSFPVTGNGRRTSYRHPPIPRM
                     SNTFIMKGEGNPDEILAGVGKGLLVLKMGGGEVNPTSGDFVFHVTEGYLIEKGKRSYP
                     VRNAILTGNGPEVLRDIDAVGDDLHYESGVCGKSGQHVPVTDGQPTIRIRRLVVGGSE
                     A"
     gene            complement(11152..12183)
                     /gene="mltG"
                     /locus_tag="PLM30_00290"
     CDS             complement(11152..12183)
                     /gene="mltG"
                     /locus_tag="PLM30_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOR53638.1"
                     /translation="MKGSLPGKVLYFVFLVISLAFFGALAQYLPQQLVIAIPMGGGEP
                     LEILVSPGMNAGEIARVFASTGLTDRPAELSRWFAKVGIDRRIRPGLYRIRRGSPWEI
                     ARQMALAEPEVSGITLFPGETFETLIARLGEAFKGALGKDELFPKELRSLLPREPRSR
                     IAFLLPETYIVTPFEGNMDELVMVASALWMQRIGKLVSDPGDGGALLLKKAIVASLVE
                     REAKQQCEKPLIAGVIRNRSAAGMKLQIDATVVYAWSLSGVELKRVLFRDLEIDSPYN
                     TYLYAGLPPAPICVPSEGSWLASLEPGEEPYLFYVAMPDGSHLFSKTFSEHSEAIKRS
                     RKAFASSQR"
     gene            complement(12186..12821)
                     /locus_tag="PLM30_00295"
     CDS             complement(12186..12821)
                     /locus_tag="PLM30_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOR53639.1"
                     /translation="MKDIASLSIEDFLEELGSSSATPGGGSASALCASIAARLTQMVA
                     SLTIGRPRFEDAWEEMTLAEEKGKALSGEFLELAMKDSEAYDLFMKALSLPKDNDQEK
                     TRRKNSMQEALKQSTLVPLRTLELAGELVANSLVVAEKGNPNAITDAGVSALLADAAA
                     RGAAMNAKINLTSIEDSAFVQDSLRRVEAELKELETEVQKVLAIVSSKLPR"
     gene            complement(12818..14068)
                     /gene="hutI"
                     /locus_tag="PLM30_00300"
     CDS             complement(12818..14068)
                     /gene="hutI"
                     /locus_tag="PLM30_00300"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOR53640.1"
                     /translation="MTMKLFRGARIYTPLATGNRVMSWEKGAILARNGFIEKVGPEEE
                     VLRDLEPAMVEQEVYFGGRCVVPGFVDPHTHMCFSELREKEFALRLSGVSYMEILASG
                     GGILSSVRSLRAASDEKILERTKKNALTALSHGTTTVEIKSGYGLDTASEIRMLRLIE
                     RAGREIPTTVIPTFMGAHAIPSEFNGDPEGFVDLLAREMIPAVSAECDPPFCDVFCEE
                     GVFTVSQSRRILEAARKHGMKLKIHADEVIDTGGAALAAELGAVSAEHLLAARGENLA
                     KMASAGVIAVLLPATAFSLRKPYADARKMLELGLTVALATDCNPGSSFTQSMPFVFSL
                     AVMNMGMTVEEALAGCTLNAAKAIESDHFCGSLEKNKMADFVVLDGETPAIMAYNSGV
                     SPVLSVYKRGECAAQRPDDWRFCR"
     gene            complement(14065..14976)
                     /gene="ftcD"
                     /locus_tag="PLM30_00305"
     CDS             complement(14065..14976)
                     /gene="ftcD"
                     /locus_tag="PLM30_00305"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOR53641.1"
                     /translation="MRIVECVPNFSEGRRKEVIDKIVEPFRNGKGFSLLDHRADENHN
                     RLVVSLAGDPEAIMDALITSAGIAFSLIDMNIHSGAHPRIGALDVVPFTPLRGVTMEE
                     CVDIAREFGKRLNEKYEVPVYFYEEAATRPERKNLENVRKGQYEGLKKAIKEPERLPD
                     VGQARLHPTAGATVVGARKFLVAFNVNLGTTRIEVAKEIAKSIRFSGGGLAHVKAIGL
                     ALEDKGMVQVSVNIVDHEKDPLYRVLELVRMEASRWGVPVVETEIYGMVPASAIYQSA
                     ARYLQVADFEPEQIIELRLLEMAGDRT"
     gene            complement(15044..16576)
                     /gene="hutH"
                     /locus_tag="PLM30_00310"
     CDS             complement(15044..16576)
                     /gene="hutH"
                     /locus_tag="PLM30_00310"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOR53642.1"
                     /translation="MGSSVIELNGHSLRLEDIINVAREGRKVALDRSAVAFVERGSGM
                     VRTWAKESRVIYGVTTGFGDLSSQFIPPEQSEQLQANLMTSHASGVGDPFPEEIVRAI
                     ILLRVNSLIRGFSGISLQTLSRLVDFLNIGIHPVIPCKGSVGASGDLCPLSHLGIALL
                     GLGEVFYRGRRMDTSEALRAAGLEPIVLGPKEGLAMNNGTAGMTGVASIALWEALALV
                     KMADISGAMSLEALHGVPFAFDPRTHDLRPHQGQIMVARNVRALTKNSEIMEKYGNER
                     VQDAYSLRCIPQVHGASRDALQYVQDVIQIEINSVTDNPLIFPSEGEAISGGNFHGQP
                     VALAMDFFGIAMAEIGDIAERRAARLVDGKLSGLPPFLVEHSGINSGFMIPQYVAAAL
                     VSENKVLAHPSSVDSIPTSANQEDHVSMGMYSARKGLEILDNVRRVLAIEMLLAAQGL
                     DFSKPLAPGVGTRAAYEKLREEVPYLEKDGILYPLIDTIINRMKDRSILKAVEEVTGE
                     LE"
     gene            complement(16634..17620)
                     /gene="amrS"
                     /locus_tag="PLM30_00315"
     CDS             complement(16634..17620)
                     /gene="amrS"
                     /locus_tag="PLM30_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOR53643.1"
                     /translation="MESPALFWMAAGEEVRCLLCPRECLIRPGDVGACGVRKNVAGNL
                     FSLNYGLACVAVDPIEKKPLFHWKPGSRILSLGTVGCNLFCPYCQNYHLSRFETVAGL
                     SEITPEEVLRLSRERGTPSVAFTYNEPTVWYEFVQDASQVLKKAGKGVVLVTNGYISQ
                     KPLEDLIPQVDAMNVDLKGFSDRSYARIGGTLGPVLRTIRIAVERSVHMEITHLVVPG
                     VNDSLEEFEKMVLWIAGVSKRIPLHISRYFPAYHWDRPPTVLSDIEAREEIASRALDH
                     VYAGNLPRRENTFCPACGTLLIERDRSGRVKTHFTDNGACPRCNTSTGIVKS"
     gene            complement(17623..18201)
                     /gene="amrA"
                     /locus_tag="PLM30_00320"
     CDS             complement(17623..18201)
                     /gene="amrA"
                     /locus_tag="PLM30_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOR53644.1"
                     /translation="MGHPEGERPSPHPYVLLAKRAVCLTASRLDPFLYIDRGQYCAMP
                     EMTVRRGCFVSIKKGPHGELRGCIGTIRPVRENLWEEIVANARAAASEDPRFPPLEPG
                     ETAECLVSVDILDEPAPIEDLSELDPFLYGVIVERGGRKGVLLPDLEGVDTVEQQISI
                     AMRKAGIGPEGEVVIYRFKVSRFSEVDLERVD"
     gene            complement(18201..18986)
                     /locus_tag="PLM30_00325"
     CDS             complement(18201..18986)
                     /locus_tag="PLM30_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HOR53645.1"
                     /translation="MGFLLPHAPILVPEVAARSGAQTGKTLEALISLGGSLKRLAPDL
                     LLVLDPHAATERILTFVNADRFVGDLGEFGARSVSLSFGGAGEEGDMLFSYLHPHFPS
                     RYYRPGVHDLDYAAVVSLHLLLPLLGVVPSMVLVNPVTIDFRQAFELGRLLRGYPSEK
                     KWGLLASGDLSHRLTKSAPSGFHPDGQVLDRSIVEALRASSPDPIFNLPGTVIQNAGE
                     CGLRSVLALIGLSMGEEIRFLSYEAPFGVGYASALLECSGEVS"
     gene            19185..19574
                     /locus_tag="PLM30_00330"
     CDS             19185..19574
                     /locus_tag="PLM30_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOR53646.1"
                     /translation="MEMISEIEEMVDSIGYPPRAPGGEGIPKRPVSWAVANVLSLPLS
                     QAARILLISSLLGSDGRDVDCRFNELASLSGLSERTAYRALKEIKGSGLFEIQVTPGS
                     GVRISAGWLWDEPRERARDFFQKAALR"
     gene            complement(19592..20689)
                     /gene="queA"
                     /locus_tag="PLM30_00335"
     CDS             complement(19592..20689)
                     /gene="queA"
                     /locus_tag="PLM30_00335"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOR53647.1"
                     /translation="MDRQHRKGLTPPEGPPVGVDNPFDTNIYHYDLPKSMIAQTPAEP
                     RDSSRLMVVNRNSGTIRHQTFREIACYLRGTDLLVLNDTRVVKARLHAVKPGGGARVE
                     VFLLRPLEGEGSVLWETLIRPGRRVKPGQRLILDGGIEVVIGEGSGQGARVCRFPPEI
                     DVWGLMEEVGEMPFPPYVHNPFIDPERYQTVYGTRQGSVAAPTAGLHFTPDLIEGITS
                     RGVKLASITLNVGLGTFRPVEEEDIRMHRMHTEECSIPDDTALAVKETREAGGRVIAV
                     GTTVVRALESRATREGLVVPGSFSTDAFYYPGYRFQAIDAMITNFHLPRSTLLMLVCA
                     FAGKELVMRAYNEAIERRYRFFSFGDAMMIL"
     gene            complement(20691..>21722)
                     /locus_tag="PLM30_00340"
     CDS             complement(20691..>21722)
                     /locus_tag="PLM30_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOR53648.1"
                     /translation="FNVINVLGIEDYLKGVLKMEVNPAWPLESLKAQAIIARTYALRN
                     KNRHGSSGFDLCALNHCQVYRGVNAEDPILSKAVDLTRGIVLRYQGAYAATFYHADSG
                     GHTADVSSVWGSSIPYLIGKPEPQKYQSPYSNWQVSMGLSEIQNILASRGVRVGNLTG
                     IAVSSRDRAGRAVNLTLTGTGGQTTIKASQFRTMIGPDRVRSTFFTIGGNPPGDPLPM
                     PTCAPGPANEESLPLSAKDEQMLIAFTQQGVFNTEELMDMLIKPQRRGFYLRKALEKQ
                     SGVGATAKAPSSTPSFVGGRVTFTGRGWGHGVGLSQWGAKSLAEAGWDCKRILRHYFP
                     GTELSRTSN"
BASE COUNT         4336 a         6532 c         6223 g         4631 t
ORIGIN      
        1 tcaacccagc tcttgccgat tcgcttcaag cagcgatctc gccagtcccg cggcttccgg
       61 gtcatcgggg tcgcccgcca gcatcctggc tagctccctc tccctctgtt tcccttcgac
      121 ctcaaggacc ctgcttatat cgccctccct ttccacccgg aagtgctggt cggccaggga
      181 cgcgatcgag gcctcgtggg ttatgaggat cacctggcat cgctttgaca ggtctctcaa
      241 ggtttgtccc gccaggaagg cggaacgccc ccccaagccc gcctctacct catcgaatac
      301 cagggtttgg ggaagcattt cgtcggaggc ggccgcttgg agggccagca atatcctgct
      361 gagttcacct cccgatgcga tcttggaaac ggggccctca aggaatttat cgtcggtaag
      421 gtagaaagca atgctgtcgg ccccactctg ccggaccttc tccagtggct gcagcgacac
      481 cccgaaccga agcccctcca tggccaggcc ctgtagagag acgttgaccg ccatctctag
      541 cttctcggcg gcttccttcc tggatcctct gagagaaagg gcaagggcgg acacttcctt
      601 tttcagggaa gcggatcttt cttgcagcct ggcaagggtc tcgcggctgt ccctgagcca
      661 ttccagctcc gagtgcactc tttcggcgaa gtccacaaga tcctcggcgt tatcgacctt
      721 ggcatatctc atcagttttt tcaggctccc cagtcttttc tccagttttt cctgttcctc
      781 ctggagggat tgttcccttt ccacggagaa gacacctttc agtccgctct cgatctcctg
      841 agtggaagat aggagccttt cgatactccc ttcgagttcg gggatctcgt tcaggctttg
      901 cctgacctca tggcaaaact gctcgatggc ggaggaaagg ccctccccgg agctcccgcc
      961 agtcagcctg tcatgggcga gtctcagcag gccctgtttt ttcagttgtt ccgaaacacg
     1021 gttcatatcg gtctcccact ctgactcgca cccgggcctg atacccattc tgctgaactt
     1081 ctccagaaag gcttcgccct cctggtacct cgattcgatc tccctcctcc ggcgggtgat
     1141 ctccaggagg cccctttcat tccccagggc cagtgaaacc gcctgggaaa ggtttgaccg
     1201 gatagaccgg agagggtcac cgccactgtt gtcaagcagt tcagcctgtt ggtccggttc
     1261 caggagttca agctgtgcga actggctctg gatggctata acctttgaaa gggccccgga
     1321 aagcgtgttc aggggcactg gtttctcctg gaggtaggtt ttggccctcc cggccgatga
     1381 aaccacccgc ttgacaagga taaaaccctc ttggggctga aggtcttcgg gcagagaggc
     1441 cagcggggca tccatttcca gggctgccct gacttcggcc tcctcaacgc cggccctgat
     1501 gatcgacgat tgggccctct tgccgctgac gagttcaaga gccctgacca ggctgctctt
     1561 cccggccccg ctttccccgg taatgacgat aaaattgccc ctgaaatgga gagaggcctc
     1621 tcttatcccc cccacgttgg tcaccatcag ttcggaaagc atgggatcac tcctcgagaa
     1681 tgctgggcgt tctgtgcccc cattggaatt tttcctgaag aagggtgaag aattcgttct
     1741 ccgggagggt gataatgccg atcgtcctgt cctggtcaag agagatggag atgcggtctc
     1801 cggggagaag ttcatatccc aactggccgt cctgggtcag catgatctct ctgtgctgac
     1861 cagccggggt aattgtcacg gtgtccgact cgctgagcac gatcggcctc gagtagaggg
     1921 tatgagcgca tatcggggtg aggatcatgc aggacaggtg aggcgggacg atcgggcctc
     1981 cggcggaaag ggaataggcc gtcgaacccg tgggagtcgc cacgattatc ccgtcggcgt
     2041 gcaacaggtt cagcggcctt ccgctgacgg ctatatcaag ggcgatcatc ctcgcgaagg
     2101 gccccttggt aacgacaagg tcgttcaggg cgtacacttc gtgcttgtgt cttttccccc
     2161 tggagacgat gccgtgcagg aggggcctcc ggtttatcga gtaatccccc gccatgatct
     2221 tttccagatc ctcctccgca ctctctatct tgccgcagga caggaaaccg agacgcccga
     2281 cgttgatccc gtagaggggg atatcgtgac ctataacgta acgggctgcc cgcaggaaag
     2341 tcccgtcccc tcccaggacg acggcggcgt cgaccctctc tttccattcc tggtccgagg
     2401 tttcctgctc tcccaacaca tgggactcgt ggggagggag ggcgaaattg acgtcccgtt
     2461 ccctgcccca ccggagaatg ctctcggcaa gggccagggc cgcacccttc tgggtattga
     2521 ccaaaagccc aagcgttttg atcatccttg actcccccct tttgatgcct ttttgtccct
     2581 gacttcaaca agctcgcggt gagccttttc taccaggtcc ccaaagaagg cctcatcgag
     2641 gcccgccggg gggacccctc ctttcctgag gtggaacagg aactctatat tcccgctggg
     2701 ccctagtatg ggagaaaaat cggcgcccat caaggccatc ttatccatgg agcgggtaaa
     2761 aaggcttaac gatatcagga tactcctgtg caggcgccta tcccttacga cccctttctt
     2821 cccgacaaga cctttgccgg cttcgtactg gggcttgacc agggcgatac aatcgccgtc
     2881 gggcgccagg agcgcttcca cggccggcag gatcttggtg acggagatga aggatacatc
     2941 catggtggct atctcgggga cgtcccgcag gtggccgggt tcgaggtatc tggcgttggt
     3001 cctttccatg acgacgaccc tctcgtcgtt cctcagccgc caggccagtt gtccgtaacc
     3061 gacatcgaca cagtataccc tcttggcccc gcgatcgagg agcacatcgg tgaacccccc
     3121 cgtagaggcg ccgatatcga tgcatattct ccccgaagga tcgacgggga acacgtccaa
     3181 ggccttcagg agcttgtaag cccccctgcc ggcccatcgg cactcaccgc tgatctcgat
     3241 aatggcatcg ggcgatacct ttgcccccgg tctgacgagc ttttccccgt tcacccgtac
     3301 atttccggcc attacgaggg cctgcgcccg gttcctgctc tcgacgatcc cccttttgac
     3361 caactccagg tccagccttt catctccctt tgaaggtttc ataaattttt aacacctctt
     3421 ccgggacaag cccgcaatgc tcccactgtt cgcggaccgt cccgtgggga atgaaccggt
     3481 cggggacacc caggcagttt acgcgggagg cgaccctcac ccggtcagcg aaaagagata
     3541 tcttctcccc gatacccccc tcgatgttgc cgtcctcaag cacgacggcc aaggggtgcg
     3601 tacgaagggc ctcctcaacg gcatcgacat ccagcggttt caaaaagcgc agatcataga
     3661 cggaaggtgg ttccacgccc gtcttttcgg ccttctcaaa gaccgaaagg gccaaaggga
     3721 tcgtcgcacc gtagctgaaa aggcaccatc cagtcccttt gagtatcctt tcggcccggc
     3781 cccagacttg ccggggggta tccccttcac gacatatcga tgcaggggcg gccccacgcg
     3841 gcaaccggat agccagagga gaaccgtttt tcagagcctc ccggtagatc catttcagat
     3901 cctccaggtc ccggggggcc ataacgttca ggttggggat cgcccgggtc catggcagat
     3961 ccaggacccc gtggtgggtc tcgccgtcct cgcctaccag gccggcacgg tcgatcgcca
     4021 ggatgacatg gagatcctgc atgcagatgt catggaccag ttggtccatg gctctttgta
     4081 gaaaggtgga atagatggag accacgggta aaagccctgc cgacgccatt ccggcggcaa
     4141 aggtcagcag gtgcccctcg gcgatcccca catcgaaaaa cctttccggg tactttttct
     4201 ggaaagggcc cagtttgctg ccttctttca tggctgccgt gagaaggacc accctctggt
     4261 ccaagcccgc cagctcctca atgcatgccg ctgaggcttc gctccaactt cccggcgagg
     4321 gttcgccggg ggtgagggcc gaaggacaag gggaaatgcc atgaaatttc tccgagtcct
     4381 cctcggctgg tttgaagccc tttccttttg tggtcaaaag gtggaccagg agggggtaac
     4441 cgtagccctt cgccatggag aagatctttt ccatctcctg gatatcgtga ccgtcaaagg
     4501 gtccccagta gcttataccc aggtcttcga aaatgttggg cggcagcagg aacctcttca
     4561 gggtctgctt ggacctttca agcagggatt ctagtttctt cccctcgggt accctgtgac
     4621 agaagtactt gagaaaccgc ttgaactgcc tatacatggg gttcacgctc agggcggcaa
     4681 ggtggctggc catcccgccc acccttttgc ttatggacat ctggttgtca tttagtatga
     4741 agatcacggg cgtatcgagt tcatggacat ggttgagggc ctcgagggca agtccgttga
     4801 tgagcgatcc gtcgcctatc accgccacca cgtggtgatc ctggcccaag aggtcccttg
     4861 ccttcgcctg gcctagtgcg gcggatagtg atttgctgct gtggcccgcg ttgaagtgat
     4921 cgaaagggct ctccttagga tcgggaaagc ccgatatacc cttccacttt ctcagcgttt
     4981 tgaaccggtc ttttctgccc gtgaggatct tgtaggcgta ggcctggtgc cccacgtcga
     5041 aaatgaccct gtccttcgag ggatcgaagg atctcagcag cgctatattg agctcaacgg
     5101 ccccaaggga ggaagcaaga tgtccaccgt tggattcgac cgtttttatg atcatctccc
     5161 tgatctcctt cgccaggaga ccgagcccgg ggtgatcgag accttccagg tccctgtagt
     5221 ccactaatga tcccagcaga tccttcatcc tttcagttca acccctaagg gatcctaccg
     5281 gtctctaccc gccagcatct ccggaaacct ttccagtgct gccacgtccc tttctatcgc
     5341 ggagagcgct ttttgagcca gggacgtctc ttcctgggcc attctttttg cctcctcaag
     5401 gccgaaaacg ctcacgaagg tgatcttgcc ccgggtgctg tccttgccgg gtattttgcc
     5461 ggtctttgag gcctgccccg tgacatcgat gatgtcgtcc gctatctgga aggccgtacc
     5521 aaggtgatcg ccgaacacag cgaagagccg cctctcgtcg ggctcggccc ccccaaggat
     5581 ggcccccgcc tggacgcagg cactcaaaag gaccgccgtc ttggacctgg cgacggtcca
     5641 ggggtgttct tccctcgagc cgacgctcaa tgggtccgtg tcgaggacct ggcccccgca
     5701 gatcccgaaa ggtcccgcgg catcaaggat cacccccagg gcttccagga tggaatgaca
     5761 agagaaggct ccactcgcga ggagtccagc acgggcgaat tcgaaggccc agatgaagag
     5821 ggcgtccccc gccaaaagtg ccagggatcc cccgaaaacg gcgtgattgg aaggtttccc
     5881 cctcctcagg gcggcatcgt ccatttcagg gaggtcatca tggatgaggg aagcggtatg
     5941 gatcatttcg caagccaggg ccatcggcaa aagatcctcc cgggaaaccc cgaaggcttc
     6001 cccggacaga acgcaaagga ggggcctgat ccttttcccg cccgcgagaa gcgagtaggt
     6061 cattgaatcc cataaccttt gggggatgcc gggcgggttc ttttcacaaa ggtcgagaag
     6121 ggctttttct atgaaaagtc ggccttcgga gatcctggcc tccgggtcgt cgttagggga
     6181 aggcccttta gtcgccatcg gccggttcat ccccggatag aggatcccac tccctcccct
     6241 ccgactcccc ctgctcagcc gagaggaggg tgatcctctg ttgagcctcc tccaggtatt
     6301 tcctgcagga attgaccaac cctatccccc gctcaaagag ctccagcgaa tcctcaaggg
     6361 gtatctggcc cgagtccagc cgcgacacaa tctcttgcag ttcggccata tcttttgtga
     6421 aggacatccc aagatcactc cagtcaaagg ggactcgcct gaacgggacc atgatacaat
     6481 gaaacaccat tcttgcgctc cccgatccac ccgtggtaca atgctcaccg tttgcccgac
     6541 atgcagggac aggaggaatg ataatggcca atgtctgcga ttgttgcgga agaggccggg
     6601 gcgccggcaa cagggtcagt cactcgaacc gtcactccag gaggacctgg ggcattaacc
     6661 ttcagaccgt aaaggtcgac ctcggcggcg gcgaatcacg gaggatgaag atctgcgcga
     6721 aatgcctccg ttcaggaagg gtcaggaggg ccgtctagtt tcggcccttc tgcttttgct
     6781 cccgctcggc atattcacgg gtcgcccaat aaatgccgag ccagcccgag gagagaccca
     6841 cctccacggc ggcctcggct acgttgctga cagcgaaggg tttcttcagg tagagactcc
     6901 cctccccaat agtccatttg gttccagtca tccttacccc gaggcatttc tccgaaagcg
     6961 ccagcgttga aaagatcccc cctttttcca ggcccgacag gcgaagcgtc tcaccgcttt
     7021 ttagtatgaa aagggcctcg gactcatcgg cctcgcagcg caccttcact ccccattcct
     7081 cttccgccca gataagggag tagaggttgg cgaaaaggtg gtcgaaccgt cccccgaaag
     7141 ctcctgtcag gatggcggta catccacgcg ttccattcgg gccgccgcaa gtaccgccga
     7201 cctccttcag ggcaagctgg aggtccgtga ggtccttttc aggcgggaaa aggagagaag
     7261 gtattttttc ctcccgtgac cattgccggg cctcctccga aacgctgtcg ttgtcaccga
     7321 tgaaaaagga gggccttatg cccgccgcca tgcaactgtc gagcccgcgg tccaccgccc
     7381 aaacatcctt ttccctgcac agctctctca accaggaagg ctctggcgtc ctcccccccg
     7441 ccacgaacac tatagaaggg ggctcgaaag ggggcgtaaa ctgggccttg agttgggaga
     7501 ggaagatcaa cgccatcggg ttaccgtccc gtcaaaggtt gttggtgctg gcctcgagga
     7561 tctgcctggc ttcttcctcg tcaacgagga tgtccctcgg tttcgatcct tcctggggtc
     7621 ccacgatacc tacctgttcc agggtatcga tcagcctcgc cgccctggta aaacctatcc
     7681 ttaactggcg ctggagccgg ctggccgagg cgataccgct ctgcagtatc aggttcatgg
     7741 cgtcctccag gaggggatcg tcgagatgcg ctttcactcc attggtctcc cccgtgtcct
     7801 ccatatcgat gtcaaaaaag acagggtcgc cgaagagggt cgaaaggtaa tccataaaac
     7861 gcaccatcac aggctcgtcc atccagggcg actggatcct gagcggtttg ggaagcctgg
     7921 gggtgaggaa gagcatgtct ccgctgccaa ggagcctttc ggccccggaa atatcgatga
     7981 cggtcctcga gtccgtctgg gaaggtaacg taaaggccac ccgcgctggg atgttggcct
     8041 tgatgaggcc ggtcacgacg ttcacggaag gcctctgggt ggcgatgatc aggtggatgc
     8101 ccgtggcccg ggccatctgg gccagccggc agatgtaatc ctccacttcc cggggcgatg
     8161 tgaacatcag gtcggccagt tcatcgatca cgataatgat ataagggagc cgatccaggg
     8221 ggagagctct cgcgttgaag gccctgatgt tacgcaccct ggcctgggcg aacatttcgt
     8281 acctcctctc catctccctg atcgtccacc ccagcgcgtg aacagcctgt ctggactcga
     8341 cgacgggggg ggttatgttg tgagggaggc ggtcgaagat cttcatctcg acccttttgg
     8401 ggtctatcag gaggaaacgg agctcttcgg gcttcatgcg acaggccagg gccgcgatgc
     8461 agcatgaaac gaagacgctc ttccctgaac cggtagtccc ggcgatcagc aggtggggca
     8521 ttttttcaag gccgatggtc aagtggcgac cgtccacgcc gacccctacg ggtatggcta
     8581 ggtcgcagtc ggcctcctgg aaggaggggt gatccagtac ggtcctgata ttcaccggtt
     8641 tcctctcggg gttagggacc tctatgccga taaaggtctt ccccggtatc ggcgcctcga
     8701 tcctgaggct ggggacggcc agggatacgg ccaggtcgtt ggcgaggctt gataccctgc
     8761 tgaccttgat gccgggcgcg agttggatcc ggtactggat gaccgtgggc ccgacgacgg
     8821 tttcggccag ttcggccgcg atgccgaatt catccagggt ttcgatgatg ttttctccct
     8881 gttctgaaag caacctcttg tccaggctga aggaaaaatc atcgggcggg ccgaagatgt
     8941 ccaaggggac agggaaaaca agtccctgcc cgggaggagt cttggggaaa gccccggcct
     9001 ggtcgggagc atcttcgtcc actatcgtat cggggatatc gatctggccc cgggatgccg
     9061 tggggctgat ccccggtttt ttcgcgtcgg ccctcgggtc gatgcggctt acgccttttc
     9121 tcggttctac cgatggggaa acggattttt ccggcctctc tctcggcgag ggcgagagga
     9181 cgttaccgaa agctctgaga acacgggaaa ggaaagaggc cgcctttgtc atactcttct
     9241 tgttgatgat cccataaagg gtaagggctg cggcgatacc gccgaaaccg gccaggacgg
     9301 ccccgagagg tccgatgtgc ctgagaagta taccggccag gaaggacccc acaaggcctg
     9361 gcctcaaaag tgcgaccgcc ggttcgatgc cgctcagggc tgaaaggccg aggaagaggg
     9421 cgcagcatag gtaaagtatc gatgaagcca gggtttgctt cagggggaag ggaatggggt
     9481 accctaggac ccgggaggca ccaagataag cgccaaagac cagggggacg ataatgcctc
     9541 cgccgaaggc ctccagggtc caggaatgga tcacctcgcc gaattccccg gtcctgaaga
     9601 ggaaaagggt ggccagaagg tacagatcta ccagcaggaa ggcgaaaacg gccatggtga
     9661 ccccgggctg cttcctgcct cccttcatgt cttccttcct gcccgggctt ttcgagccgg
     9721 tcttgtcccg ggctttgggt ttcctcctgg tgaccttcat atcaagcctc gctgcctccc
     9781 actaccagtc ttctgatcct tatcgtcggt tgcccgtcgg ttaccgggac atgctggccc
     9841 gatttcccgc atacccccga ttcataatga agatcatccc ccaccgcgtc gatgtctctt
     9901 aacacttccg ggccgttgcc ggtcaggatc gcgttcctga cagggtatga tcttttaccc
     9961 ttctcgatga ggtaaccttc ggtgacgtga aatacgaaat cccccgatgt ggggttgacc
    10021 tcaccgcccc ccatcttcag cacgaggagc ccttttccca cgccggcaag gatctcatcg
    10081 gggttccctt cgcccttcat tataaaggta ttggacattc ttgggatcgg ggggtgcctg
    10141 tatgacgttc tcctcccgtt acccgtgacc gggaaggacc cttcttttga agtgagcaga
    10201 tcggtaagga aggagaccag gacgccttcc tttatgagag cggtcctcct ggaaggggta
    10261 ccttcatcgt caaaggggta actcccgtaa agcccgaaaa gggtcgggtc atcggccagg
    10321 gtgaccaggg atgacgcgac ccttttcccc agggaacccc tgaaggaaga atgatccttc
    10381 agaacgatat cggcctcaag gctgtggccg caggcctcat ggaccatggt cccccccgct
    10441 ttgccagcca aaacaactgg cattgccgac gcgggacagg gcttcgcctg tgagagcagc
    10501 aaagccctac ggaaggcttc acgggctaca tcttcaaggt ctacgatctt taggaattca
    10561 tcctggggta ggctgaaagc ctttgcagcc cttcccgaga agagagagcc atcagcctct
    10621 actacgatat tcaccgagaa tcgggtggaa aagtccgatc tccggcacag ggggttcgca
    10681 ctccctgcaa tagccacttc cccaacgccg ctgctccagg cgaaagtggc ctgcctgatc
    10741 gaggatgata atttccgtac cacccggtca aggtcccgga tcctgtcgaa gggtgcttcc
    10801 tcgttcatca ccggaaggtg caaaaggggt ctttccccct tcgggagccg tttcccccgg
    10861 gaaagcccgg cgcgcctttt cccttcggcc agcgatagag cgaccgagga caggtctgtc
    10921 cccgatgagt gggtgaagta agtcctgtca cccaccacca acctgatccc ggaaccttcc
    10981 gtgatcgatg aggagacctc ctcgaacttt ccgtcgtcca tcaggcagaa cctggcgagg
    11041 cccctctgga tgaaggcttc cccgaaggag gtcccgggta tcgaaagagc atcggcaaca
    11101 tcgaacaatt tatccatcaa acaaaggagc ctcctcccag ctcttgacgg ttcatctttg
    11161 agacgacgcg aaagctttcc tcgaacgctt gatcgcctcg gagtgctcgg aaaaggtttt
    11221 gctgaaaagg tgactcccgt cgggcatggc aacataaaag aggtaaggtt cttccccggg
    11281 ttcaagggaa gccagccagg acccctcgga gggaacgcag atcggggccg gggggagtcc
    11341 ggcgtataga taggtattgt aaggagagtc aatctcgagg tcccggaaaa ggaccctttt
    11401 cagttccact ccactcaagg accaggcata aacgacggtg gcgtctatct ggagtttcat
    11461 gcccgccgcg gaccggttcc gtatcactcc tgctatcagg ggtttttcac actgctgctt
    11521 cgcctccctt tcgacaaggg aggctacgat ggcctttttc aggaggagcg ctcccccatc
    11581 cccggggtcg gagaccagtt tcccgatcct ctgcatccag agcgccgacg cgaccatgac
    11641 cagctcgtcc atattgccct cgaagggggt cacgatgtag gtctcaggca gaagaaaagc
    11701 gatcctggac cgcggttccc gaggcagtaa ggaccggagt tcctttggga aaagttcgtc
    11761 cttcccgagg gcgcctttga aggcttctcc aagcctggcg atgagggttt caaaggtctc
    11821 ccccgggaaa agggtgatcc ccgacacctc cggttccgcc agggccatct gccgggcgat
    11881 ctcccagggg gatcccctac ggatccggta cagcccgggc cttattctcc ggtctatacc
    11941 aaccttcgcg aaccatcgag acagttcagc cggccggtcc gtcaggcctg tcgaggcgaa
    12001 gacacgggcg atctccccgg cgttcatacc ggggctgaca aggatctcca ggggttcccc
    12061 gccgcccatc gggatggcaa tgaccagttg ttggggcagg tattgagcta gagccccgaa
    12121 aaaggccagg gagatcacca ggaagacaaa gtaaagaacc ttgccgggca aggaaccctt
    12181 cattttcaac gggggagctt cgaagaaacg atggcgagga ctttttggac ctccgtttcc
    12241 agctccttga gttcggcctc gacgcggcga aggctgtcct ggacaaaagc agagtcctcg
    12301 atggaggtca ggttgatctt cgcgttcatt gccgcgcccc tggcggcggc atcagccagc
    12361 agtgccgaaa cgccggcgtc agtgatggcg ttcgggttgc ccttctctgc cacgacgagg
    12421 ctgttggcga ccagctcccc ggcaagctcg agcgtccgca gcggaacaag ggtggactgc
    12481 ttcagcgctt cctgcatcga gttctttcgc ctggtttttt cctggtcgtt gtccttcggc
    12541 agggagagag ccttcatgaa taggtcgtag gcctcgctgt ccttcatagc caattcaaga
    12601 aactcccctg aaagggcttt ccctttttcc tcggccaggg tcatttcttc ccaggcatct
    12661 tcgaagcggg ggcgcccaat ggtcaggctg gccaccatct gggtcaggcg cgcggcgatc
    12721 gaggcgcaca gggcggaagc ggatcccccc cctggggtag cacttgagga accgagttcc
    12781 tcgagaaaat cctcgatgga aagggatgct atatccttca tcggcaaaac ctccagtcgt
    12841 ccggtctttg ggctgcgcac tcccctctct tgtagacgga gaggaccggg gataccccgc
    12901 tattataggc cattatggca ggagtttcgc cgtccagcac aacaaagtcc gccatcttgt
    12961 tcttttcgag gctaccgcag aaatgatcgg attcgatggc cttggcggca ttgagcgtac
    13021 aaccggccag ggcttcctct accgtcatac ccatgttcat gacggccagg ctgaagacga
    13081 agggcattga ctgggtaaag gatgagcctg ggttgcaatc cgtggcaaga gctaccgtca
    13141 ggccgagttc aagcatcttc ctggcatcgg catagggctt ccgaaggctg aaggccgtgg
    13201 ccgggagcag gacggcgatc actcccgccg aagccatttt ggcaaggttt tctcccctgg
    13261 cggcgagtag atgctccgcc gagacggcac cgagctccgc cgccagcgcg gcgccgcccg
    13321 tgtcgatcac ttcgtcggcg tggatcttca gcttcatgcc gtgcttcctg gcggcttcca
    13381 ggatcctcct gctctgggac actgtaaaga cgccttcttc gcagaacacg tcgcaaaaag
    13441 gaggatcgca ctcggcgctc acggccggta tcatctccct ggccaggagg tcgacaaaac
    13501 cctcggggtc gccgttgaac tccgagggta tggcatgggc gcccatgaag gtagggatca
    13561 ccgtggtagg tatctccctt cccgcccttt cgatgagcct gagcatcctg atttcagagg
    13621 ccgtgtccag cccgtaaccg ctctttatct ccacggtggt ggttccatgg gaaagggcgg
    13681 tgagagcgtt tttcttcgtc ctctccagga tcttctcatc gctcgcagct ctcagggatc
    13741 gaacggacga aaggataccg ccccccgaag cgagtatctc catgtaggac accccggaga
    13801 ggcgcagggc gaattccttc tccctcagtt cggaaaaaca catgtgcgta tggggatcca
    13861 cgaaccccgg aacgacgcat cttcccccga agtagacttc ctgctcgacc atggctggtt
    13921 cgaggtcgcg caggacctcc tcctcggggc cgaccttctc aataaaaccg ttcctggcca
    13981 ggatcgcccc tttttcccag gacattaccc tgttcccggt cgcaaggggg gtatagatcc
    14041 gggccccgcg gaaaagtttc atcgtcatgt tctgtcaccg gccatctcga ggagtctgag
    14101 ctcgattatc tgctcgggct cgaaatcggc cacctgtaaa taacgggcgg cgctctggta
    14161 gatcgccgag gccggtacca taccgtagat ctcggtctcg acgacgggga cgccccacct
    14221 tgaagcctcc atacggacca gttcgagaac cctgtagagg gggtcctttt catggtccac
    14281 gatgttcacg ctgacctgta ccattccctt gtcctccagg gcgaggccga tggccttgac
    14341 gtgggccagc ccccccccgg agaacctgat ggacttggcg atctccttgg cgacctcgat
    14401 ccgggtggta cccaggttga cgttgaaggc caccaaaaac ttcctggccc ccaccaccgt
    14461 cgcccccgcc gtgggatgca accgggcctg cccgacgtcg gggagtcttt cgggttcttt
    14521 tatggccttt ttcaaacctt cgtactgacc tttccggaca ttctccaggt tcttcctttc
    14581 cggcctcgtg gcggcttcct cgtagaagta gaccgggacc tcgtactttt cgttcagccg
    14641 ttttccgaat tctcttgcga tgtcgacgca ttcctccatg gtcacgcccc tcaacggggt
    14701 aaaggggacc acgtcaaggg caccgatccg tggatgcgca ccggaatgga tgttcatgtc
    14761 gatgagcgaa aaggcgatcc ccgccgacgt tatgagagca tccattattg cttcggggtc
    14821 gccggcaagg ctgaccacga gcctgttatg gttctcgtcg gcccggtgat ccagcaagct
    14881 gaaccccttc ccgttacgga agggttccac gatcttgtcg atcacttcct ttcttcttcc
    14941 ttcgctgaag tttgggacgc actccactat tctcatgttc cgcctccatc atccatcggt
    15001 gcctcgccaa ggggggggca ccggtcattt tttcacccgg aggtcattcc agttcccccg
    15061 tgacctcctc cacggccttg aggatactgc ggtccttcat cctgttgatg atagtgtcta
    15121 tcaggggata gaggatgccg tccttttcaa ggtagggcac ttcctcccgg agtttctcgt
    15181 aggctgccct tgtgccgaca ccgggggcaa ggggtttcga gaagtccagt ccctgggcgg
    15241 cgagaagcat ttcaatggcg aggactctcc tgacattgtc aaggatctcc aggcccttcc
    15301 tggcagaata catccccatc gagacgtggt cctcctggtt cgccgaggtc ggtatcgagt
    15361 ccaccgaaga ggggtgggcc aacaccttgt tctccgatac aagagccgcg gcgacatact
    15421 gggggatcat gaagccgctg ttgatgccgc tatgttccac caggaagggg gggagccctg
    15481 aaagttttcc gtccacaagc ctggctgccc ttctctcggc gatgtccccg atctcggcca
    15541 tggcgatacc gaaaaagtcc atcgcgaggg ccacgggctg gccatggaag ttcccgccgc
    15601 tgatggcctc accctcggac gggaagatca aggggttgtc cgtgacggaa ttgatctcga
    15661 tctggataac gtcctggacg tactggagag catctcggct cgccccgtgg acctggggga
    15721 tacagcgaag ggagtaggcg tcctggaccc tttcgtttcc gtatttttcc atgatctcgc
    15781 tattcttcgt aagggctctt acgttcctgg ccaccatgat ctgcccctgg tgtggtctca
    15841 ggtcatgggt cctgggatcg aaggcaaagg ggacaccatg aagggcctcc agagacatgg
    15901 ccccggatat gtccgccatc tttacaaggg cgagggcttc ccacaacgct atggaggcga
    15961 caccggtcat gcctgccgtg ccgttgttca tggcgaggcc ttctttgggc cccagaacga
    16021 tcggctccag cccggcggct ctgagggcct cggaggtatc catcctcctg ccccggtaaa
    16081 agacctcacc caggccaaga agcgctatgc caagatggga cagcgggcac aggtcgccgc
    16141 tggcaccgac ggagcccttg cagggaataa cagggtgtat gccgatgtta aggaaatcga
    16201 caagcctgga aagggtctgg agactgatgc cggaaaaacc ccttatcagg gagttcaccc
    16261 tgagcagtat gatggctctg acgatctctt cagggaatgg gtctcccacc ccggaggcgt
    16321 ggcttgtcat gaggttcgcc tgcagttgct cgctctgttc cggtgggatg aactggctgg
    16381 aaaggtctcc gaagccggta gtaacgccgt agatgaccct gctctccttg gcccaggtac
    16441 ggaccatacc ggaccctctc tcgacaaagg ccaccgcgga gcggtcaagg gcaacttttc
    16501 tcccctcccg ggctacgttt atgatatctt caagcctcaa ggagtggccg ttcagctcta
    16561 taacggaaga ccccataaag gatcaccccc ataatttgtt caaaaccctt ctgcgaacag
    16621 agtatatcgt aaatcacgac ttgacaatac ctgtagaggt attacagcgg gggcatgccc
    16681 cgttatcggt aaaatgagtt ttcaccctcc cggagcggtc cctttcaatg agaagtgtcc
    16741 cacaggcggg gcagaaggta ttctccctgc gtggcaggtt cccggcgtag acatggtcga
    16801 gagccctcga ggcgatctcc tcccgcgcct cgatgtcgga taggactgta ggaggccggt
    16861 cccagtggta agcagggaag tacctcgaaa tatgaagggg gatcctcttt gaaacgccgg
    16921 cgatccaaag taccattttt tcgaactctt ccaaggaatc gttgacacct ggcacgacga
    16981 ggtgggttat ctccatgtgg accgatcttt cgacggctat ccttatcgtg cgcagcaccg
    17041 ggccaagagt gccgccgatc ctggcgtagg aacgatcgga aaaacccttc aggtccacgt
    17101 tcatagcgtc gacctggggg atgaggtcct ccaacggttt ttgggagata tagccgttgg
    17161 tgacgaggac tacccctttg cccgcctttt tcaggacttg ggaggcatct tggacgaact
    17221 cgtaccatac ggtgggctca ttgtaagtga aggccaccga cggggtgccc ctctccctgg
    17281 aaaggcggag cacctcctcc ggagtgatct cggagagacc ggcgaccgtt tcgaaacggg
    17341 agaggtggta gttctggcag taaggacaga aaagattaca gcccaccgtc cccagggaga
    17401 ggatcctgct gccgggcttc cagtggaata gtggcttttt ctcgataggg tctacggcca
    17461 cacaggccaa accgtaattc agggaaaaaa ggtttccagc gacattcttc ctgacgccgc
    17521 aggcgccaac gtcaccgggc cggataaggc attccctggg acagagaaga caacggactt
    17581 cttcacccgc ggccatccag aaaagagcgg ggctttccat ggttaatcga ccctctccag
    17641 gtcgacctct gaaaaccggc tcaccttgaa ccggtatatg acaacctccc cctctggccc
    17701 gatccccgct ttcctcatgg cgatggagat ctgttgctcc acggtatcca cgccttccag
    17761 gtcgggcagg agaacccctt tcctccctcc cctttcgacg atcaccccgt aaaggaaggg
    17821 gtcaagttcg gaaaggtctt cgatcggggc aggttcatcc aggatatcca ccgaaacgag
    17881 gcattctgca gtctcgccgg gctcaagggg agggaatctt gggtcttccg aggcggcggc
    17941 ccgggcgttg gccacgatct cttcccaaag gttctcccga acgggccgga tggtcccaat
    18001 acaccccctc aattctccat ggggaccctt ttttattgat acgaagcaac cccgccttac
    18061 ggtcatttcc ggcatcgcgc agtattggcc cctatcaata taaagaaagg ggtccagccg
    18121 ggaggccgtc aggcagaccg ctcttttcgc caggaggacg taggggtgag gactaggcct
    18181 ttcaccttcc gggtgtccca tcaggacacc tcccccgaac actccaggag cgcggaggcg
    18241 tagccgacgc cgaaaggtgc ctcgtaggac aggaacctga tctcttcgcc catggaaagc
    18301 ccgataagcg caagcaccga ccgaagtccg cactcgccgg cgttctgaat gaccgttccc
    18361 gggaggttga aaatggggtc cggcgacgac gcccggagag cttccacgat cgaccggtcg
    18421 agtacctgtc catcgggatg aaaacctgaa ggggcactct tggtcagcct gtgggagagg
    18481 tccccgctgg cgagaagccc ccactttttt tctgaaggat accccctgag gagcctcccc
    18541 agttcgaagg cctgcctgaa gtctatcgtc acggggttga caaggaccat ggaaggaacc
    18601 acgcccaaga gcgggagcaa gaggtggagc gacacaaccg cggcgtaatc cagatcatgc
    18661 acaccgggcc tgtagtacct ggaggggaag tgagggtgaa ggtatgaaaa aagcatgtcg
    18721 ccctcctccc ccgctccccc gaaggatagg gataccgacc gggcgccgaa ttcgcccagg
    18781 tctccaacga acctgtcggc gttcacaaag gtaagtatcc tttcagtagc ggcatgaggg
    18841 tcgaggacca gtaggagatc gggagcgagt cttttcaggc ttccacccag ggaaatcaaa
    18901 gcctcaagag tttttccggt ctgggctccg gaccttgccg ccacttccgg aacgaggatc
    18961 ggtgcatggg gcagaaggaa gcccatcgtc cacaaggcca tccctcccaa aagttttgca
    19021 aaaggctctt ttcatgtatt atgacagcag gagtcggttt tttccatcat ggcaccgcac
    19081 tttcagggag tacccctcgt cgaagggaaa tatcggggct gtcccggggg agacaggagg
    19141 gtctggtgag aagggtcggt caacaggagg gaacctcctc cataatggaa atgatctccg
    19201 agatcgagga aatggtcgat agtataggtt acccgccacg ggcacccggg ggggaaggga
    19261 tcccgaaaag gccggtatcc tgggcagtgg ctaatgtcct atcgcttccc ctgtcccaag
    19321 ccgcccggat ccttctgata tcatccctcc tgggaagcga cgggcgtgac gttgattgcc
    19381 gcttcaacga actcgcttct ctatccggac tttcggagag gacggcttac agggctctca
    19441 aggagataaa gggatcgggc ttatttgaga tccaggtcac cccgggcagc ggcgtcagga
    19501 tatcggcagg gtggttatgg gacgagccca gggaaagggc gagggatttt ttccagaagg
    19561 ctgccttaag atagatccca agatgggccc ttcataggat catcatggcg tccccgaagg
    19621 aaaagaagcg gtaccgcctt tcgatggctt cattgtaagc cctcatgacg agttccttcc
    19681 cggcaaaagc gcagaccagc atgagcaggg tgctccgggg aaggtggaag ttcgtgatca
    19741 tggcgtcgat ggcctggaac cggtagccgg ggtaataaaa agcatcggtg ctgaatgaac
    19801 caggcaccac caggccctct ctcgttgccc tgctctcaag ggcccttacc actgtcgtcc
    19861 ccacggctat gactcttcct cccgcctctc tcgtctcttt gacggccaaa gccgtatcat
    19921 cagggataga acattcctcg gtgtgcatcc ggtgcatcct gatgtcctct tcttctaccg
    19981 gcctgaaggt tcccaacccc acattcaagg ttatactggc caacttgacc cccctggacg
    20041 ttatcccttc gatcaggtcc ggtgtgaagt gcaagcctgc cgtcggggcg gccaccgaac
    20101 cctgccgcgt tccgtagacc gtctggtacc tctcgggatc gatgaaaggg ttgtgaacgt
    20161 agggaggaaa gggcatctcg ccaacctctt ccatgagacc ccagacgtcg atctccgggg
    20221 gaaagcgaca gaccctggcg ccctgaccgc tcccctcccc gatgacgacc tcgatcccgc
    20281 catcgaggat caggcgctgt cctggtttga cccgtctacc gggcctgatg agggtttccc
    20341 aaagaactga cccctccccc tccaagggcc tgagaaggaa aacttcgacc cgggcacctc
    20401 cgcctggctt tactgcatgc aagcgggctt tgacaaccct cgtgtcgttg agtaccaaaa
    20461 gatccgttcc ccggaggtaa caagcgatct cccggaaggt ctggtgccgg atcgtgcccg
    20521 agttccggtt caccaccatg agcctcgatg aatccctggg ttcggcaggg gtctgggcga
    20581 tcatggattt ggggaggtca taatgataaa tattggtatc aaaggggtta tcgaccccga
    20641 cgggaggccc ttcgggcggg gtcaaaccct ttcggtgctg cctatccatg tcaattggac
    20701 gtcctggaga gctccgtccc ggggaagtaa tgccgcagga tccttttgca atcccagccg
    20761 gcttccgcca ggcttttcgc gccccattgg gacaggccca cgccgtgccc ccacccccgg
    20821 cccgtgaaag tgaccctgcc tccgacgaaa gatggagtgg atgagggagc cttcgcagtc
    20881 gcgcctaccc cactctgttt ttcaagggct tttctcaggt aaaaacccct tctttggggt
    20941 ttgatgagca tatccattaa ctcctcggtg ttgaatacac cctgttgggt gaaagcgatg
    21001 agcatctgtt cgtcctttgc gcttagaggg aggctttctt cattcgccgg cccaggagcg
    21061 caagttggca tggggagcgg atctccgggg gggttccccc caatggtgaa gaaggtgctc
    21121 cttaccctat cggggccgat cattgtccga aactggcttg cctttatggt ggtctgtccc
    21181 cccgtcccgg tcaaggtcag gttaactgcg cggccggctc tgtccctaga ggaaacggcg
    21241 atgcctgtaa ggttccccac cctgactccc ctcgaggcca ggatattttg tatttcggat
    21301 agtcccatgg aaacttgcca attcgagtaa ggggactggt acttttgggg ttccggcttc
    21361 cctatcaggt agggaatgct cgagccccac acggaggaga catcggcggt gtggccgccg
    21421 ctgtcagcat ggtaaaaggt ggcggcgtaa gccccctggt acctcaggac aatgcccctg
    21481 gtcaggtcca cggcttttga aaggatcgga tcctcggcat tgacccccct gtaaacctgg
    21541 caatggttca gggcgcagag gtcaaaaccg ctgcttccgt gccggttttt gttacgcaat
    21601 gcgtaggtcc tcgcgattat ggcctgcgct ttcaaggatt ccaggggcca ggcggggttc
    21661 acctccatct tcaggacgcc cttcaggtaa tcctcgatgc ccagaacgtt gatcacgttg
    21721 aa
//
LOCUS       DAOSRG010000004        21261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_2672_length_21261_cov_9.734934, whole genome shotgun sequence.
ACCESSION   DAOSRG010000004 DAOSRG010000000
VERSION     DAOSRG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 21261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21261
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_21261_cov_9.734934"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1405)
                     /locus_tag="PLM30_00345"
     CDS             complement(119..1405)
                     /locus_tag="PLM30_00345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOR53649.1"
                     /translation="MKGRVEIIVGTQWGDEGKGRVVDAMAHTVDVVARYQGGANAGHT
                     VMVEGEKYVFHLLPSGMLYSGKTCVIGNGVVIDSQQLLDELSDLQERGKDRARLVISG
                     AAHVVMPYHKKLDSMEERLRGSRKKIGTTHRGIGPCYMDKVNRCGIRVEDLLCEDALR
                     EKLSFNLEIKNMYLTRIYGEEPLAFGEIFEQAWSWGERLKPYVGDASLTIFEALERGS
                     NVLFEGAQGTLLDLDHGTYPFVTSSSPVAGGACVGAGLGPSLVGRVTGVVKAYNTRVG
                     EGPFPTELKEELGETLRERGGEFGATTGRPRRCGWLDLVALKYAVRINGIHALALTKL
                     DVLSGFEAIPVCTGYRSDEGEDLPYKGNSVFLDSVEPVYRNMEGWKMDLGACRDFQDL
                     PRPARDYIRFIEEQTGVPIVLVGVGPNREQTIVMGL"
     gene            complement(1410..2189)
                     /locus_tag="PLM30_00350"
     CDS             complement(1410..2189)
                     /locus_tag="PLM30_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOR53650.1"
                     /translation="MGKRKKAASRFLFVALCALVFPGADRCYGGFVQSPAVAVIVDDL
                     GFSLDAAKRLASIPIPVTWAIIPYQRFSQATARMAGDLGIPFLVHMPMEAKGSPGDRK
                     ALVRAGMDPEAVRVAVRNALWSLPGAAGLNNHQGSRATEDPRAMGGVMLELKAEGLFF
                     VDSRTSGGSVAYHVAVETGLPATSNRFFLDHYDEESFLREQFRKAFARARKNGGAVAI
                     CHARPGTLDFLPKLRKMTPEDVEFVTVPEYLARKSMGGWEE"
     gene            complement(2164..3366)
                     /locus_tag="PLM30_00355"
     CDS             complement(2164..3366)
                     /locus_tag="PLM30_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR53651.1"
                     /translation="MFRRARDVFIGAVVGALLVGGVLSAVAFNEMDLFKSAPFSFQNL
                     WLMKQARVILETYHVDGEEHISETELLHGAIKGMVSSLKDPYTRFVDPDELKEEEIEL
                     QGEYGGLGIYIGQRDGKTLVVSPIEDTPADRVGLKPKDQIVKIDDEVILGWPQNEVVH
                     ALRGEPGTKVVIWVRRESEDELLKFEIERESIKIQTVKSELLQDRIGLVRITQFNLKT
                     LPDFRKAVHDLTGEGTGAFLIDLRNNPGGLLNSCVEIADLLLDDGVIVSTRGRFDRAN
                     EVYYARKGTITDLPMAVLINEGSASASEILAGALKDHGRAVIVGKQSFGKGSVQTLFN
                     LSDSSGIFVTIAKYYTPAGTVIDKVGLSPDIEVEGEFVKDRESDAQMKRAILELQGLI
                     DGKKEESR"
     gene            complement(3394..4584)
                     /locus_tag="PLM30_00360"
     CDS             complement(3394..4584)
                     /locus_tag="PLM30_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOR53652.1"
                     /translation="MKGKGWFSTTVILLLFLALSPAVAWGANPPDIDSKIKDEQGRLE
                     AIEKQISFHQKQIQQARRKEQGLLDDLSRIDQSVTLLKQKVALLDLQREKTELGIREL
                     TAEIQSTEADLAKTRGYLARRFEAIYKYGGVAEFNLLLSSSSTHEALTNAYLLARMAR
                     EDERRIVTMKESKMKLESSRKQMLDKKALLVKQKESLENDRKALKNSEAQRKQSLDTL
                     TKQKALHQKAARELEESQKELQGKIRELMTQKRKMQESRAGVPPVYLPSGAKLSWPIR
                     GEISSQFGTRVHPVFKTKIMHTGLDIRAPAGTPVKAAAAGEVLFAGWLRGYGQIVILD
                     HGGDLSTVYAHLSQIRVEEGKVVKGGEVIGNVGNTGVASGPHLHFEVRVNGEAKNPVS
                     YLGR"
     gene            complement(4608..5477)
                     /locus_tag="PLM30_00365"
     CDS             complement(4608..5477)
                     /locus_tag="PLM30_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOR53653.1"
                     /translation="MIRDTWRLLSRHSGLSFLTIVTSVAVFFLVGASILFVLNTRYLV
                     ETVEGDLTIQAYVQNSEEALETVARKAISYDSVSSVKVVTAKEALERLKARLGKLAEA
                     VALLEENPLPPSVEVQVKKASFATVIARELVAMPEVEEVVYAGDIAERLQKISRFVSR
                     LSMVVLVVSLASAALVLFNTIRIGVYARKDEISKMLLVGASRSFVIFPYVLQGVLLGT
                     LGAALSILLLWFSYGMALTTLERSLPFLRLLTDRLIVLRVGAVLVLAGLSVGWICSWL
                     AASRFVRRASRSM"
     gene            complement(5482..6177)
                     /locus_tag="PLM30_00370"
     CDS             complement(5482..6177)
                     /locus_tag="PLM30_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR53654.1"
                     /translation="MEIRFAGVTKQFPPDIVALQDIFLSIDKGEFVYLVGPTGSGKTT
                     LLRLVTRELVPTKGRVTVGDVNIRKIASHNLAYLRRNIGVVFQDFRLLPDLTVHENIA
                     FVQEVIGVPFQSVQKRTNEVLDRLGLWRKRNLFPEQISGGEQQRVAIGRAIANSPSIL
                     LADEPTGNLDTDTADEILQLLLSINASGTTLVVATHNQYIVDSYRQRVIELRKGRLIR
                     DERKGRYRSDGDL"
     gene            complement(6181..7119)
                     /locus_tag="PLM30_00375"
     CDS             complement(6181..7119)
                     /locus_tag="PLM30_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR53655.1"
                     /translation="MRSTLSGFGQGLMKLGRGRGDVVLLHADMAGSEIVTPFASLFPE
                     RTVNCGASEQDMVTTAAGLALEGKKPVAIAPSAFLTARSFDQVRTCLAIPDLGVVLVG
                     THSGLSAGEDGAVHQMNQDAALMRSLPGMSVLVPSDFDSSFAMAGLAIGHPDPVYVRL
                     GRDEVPDTGPFDEAEMRIGGGRLLLVGDGVTLCACGIMVHEALKAARILSQQGINAEV
                     IDCFCLKPLPSRLILSSLRRTGCCVVAEEHNVIGGLGEAIAALSASENPVPVKSVAVS
                     DRYGQSGTPLELQEYYGLTFREIVGESAQVWSMRRR"
     gene            complement(7116..7976)
                     /locus_tag="PLM30_00380"
     CDS             complement(7116..7976)
                     /locus_tag="PLM30_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOR53656.1"
                     /translation="MKASQRRELERTALEVRKDIVRMTGVSRACHLDSSLSVVDILVY
                     LYWKVLKIRPDEPLWPERDRLVFSKDHGSPALYAVLARRGFFERKELWNYRRLGAMLQ
                     GRPEHARTPGVDAPGGSRGMAIGIANGLLLSSRMDGNDRRVFCVIGSEELREGSLWES
                     ALRAGDLGYEGLTVVVDSSAPPSAAQTLAPGKGASERKRFEAFGWRVMEADGHNFDHL
                     ERAVLRAHPGPEGLPSVIMARTIIGKGVSSAEKGPLEKPGRPLDRNGMEQALRELELA
                     SDEIEGGVKP"
     gene            complement(7981..8991)
                     /gene="csaB"
                     /locus_tag="PLM30_00385"
     CDS             complement(7981..8991)
                     /gene="csaB"
                     /locus_tag="PLM30_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOR53657.1"
                     /translation="MSRPLDVVLCGYYGFGNLGDELMAESLLGLLNKNGVPGERVAVL
                     SAAQGVTAGRGEASRVDRWSPLEVMSALRSSRTLLLGGGGLFQDSTSTRSCAYYWGVA
                     RMARLAGCRPWAFGQSIGPLERGMARFLARDALSLCRARVVRDRGSMSLLEEWDLAGE
                     IAPDPVFSMAPGFSSGSGDLLLVNVRPWRGGLPEAASLAATRAGETSGLDPIGVALSG
                     EDESLMEDLRSRGALTLSRVVRVVDASEARDLWSKARAAVGMRLHFCLISVLAGVPCL
                     AVPYDPKVRSFAEDWGLPLWDGVGLPPFPLELRHDARLDEARGALEEVFHRVLEGMLS
                     GE"
     gene            complement(8988..10898)
                     /locus_tag="PLM30_00390"
     CDS             complement(8988..10898)
                     /locus_tag="PLM30_00390"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOR53658.1"
                     /translation="MRNGRSFFFIAVLVLAALLAVAGLLPRARAERQGNVAALVTDMR
                     DVASLARESELAVPEVLDVLLGRGLTAVAVGELTGQELMAGALDLGYGSVGELLPGPR
                     PEGLFPDSAAVLLRPGSPFSSEIRAFVLKKYPGSRAIGLDRGELLVLPLSLAETLEAG
                     VFPDYPMLELLKGRGIPLVFRPGSTPGVGGEDVALAVASVLDAFPEIRAIVPAGLFVA
                     GYPDLAPLVEVLRKRGVPVSKVEFSQQIGAALLERGLFPDILPLHSVRREEILSRRLT
                     RDDLVERFVRASRERSVKILYLRPSDLHSGSRLNRFGDECAMISKRLSSLGISTGWPD
                     TMGRWGTSPFAALALGFVLVALAVRLTARFLSMEVGDISLKEAVASLALSMAIAFLSL
                     KVPVVARVSGALVASLAATEASLVALGDFRRPLRGVICGVAVAVLSGLSIASFFGTPW
                     YMLRMGTFSGVKIALLLPPLILLLHDLKRRVHPESLTQVMSRPPVWGELALVGILAAA
                     AGVMALRSGNVSFVPGWEIRFREFLEQLLVARPRTKEIFAGYPALVLWFVVRRKDIWR
                     EYREVFRLGATLAFSSVVNSFCHFHTHLLFILLRVFNGLWTGLIVGSIMAAVLVFLVL
                     PAWRRWQGVVAG"
     gene            complement(10895..11476)
                     /locus_tag="PLM30_00395"
     CDS             complement(10895..11476)
                     /locus_tag="PLM30_00395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOR53659.1"
                     /translation="MVLASASPRREALLRDLGWRFRVVPPAVEESILEDGDPGEQARR
                     LAEAKADSVFASQRSSLVVAADTIVVLGAMILGKPDHPGDAFRMLRLLSGKTHKVITG
                     VAVRWGDKSASAIEETLVTFRDLTDRQIGAYLSGNESGDKAGAYAIQGWGSLLVRSIR
                     GCYFNVVGLPLFTLSRLLEGMGIPLEDQWGERV"
     gene            complement(11506..11751)
                     /locus_tag="PLM30_00400"
     CDS             complement(11506..11751)
                     /locus_tag="PLM30_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53660.1"
                     /translation="MMAKVSYKGWWVVILCVILGSFLGVFLQGFTATERLFRNIIDVG
                     FNMKNLDLIVIDLGFRLYIRANLGSLIGGIFGVLVLR"
     gene            complement(11754..12560)
                     /locus_tag="PLM30_00405"
     CDS             complement(11754..12560)
                     /locus_tag="PLM30_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOR53661.1"
                     /translation="MYLQSAVLGLVQGITEFLPVSSSGHLVIAQDIFGWSGPALPFAV
                     VLHLATMAATVLFFWFDIMRNLKEWFQGLFSVKERGLPGWKYGWGVIAGTLITALVVL
                     LVKPVVDILFSSPLFVGFALLVTGFILRYGSRISPGVLPVSPSSGLKVGLAQGLAVFP
                     GISRSGATIVTGLRAGLSREDAFSFSFLLSLPAILGAAILEVTEAGGLSGVLTSLPKG
                     WWIGVAVAFGSGLLALSVLRRVVVRGGLGFFSLYCMIAGLLVVGLKLIRG"
     gene            complement(12564..14183)
                     /locus_tag="PLM30_00410"
     CDS             complement(12564..14183)
                     /locus_tag="PLM30_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOR53662.1"
                     /translation="MSTGLFFNLLGGVGLFLYGIKLMGESLQDLAGDRMRRLISALTS
                     SPLKGVAVGALITAVIQASGATTVMTASFVHAGLMTLKQSVGVMMGAAIGTTITAQLV
                     AFKIGDFALPLIGFGMLFAVFGRSKRQKFIGNGIVGFGLLFLGMLTMGNAMNFLRERQ
                     DIYLAFQHHPILGVLAGTALTMLVQSSSATVGLTIVMGSQGLLTLDSAIPILFGDNIG
                     TTITAVLASLGMNRSARQSALAHVLFKVIGVAIFLALVGPFKDLVLLTSSDIARQLAN
                     AHTLFNVLNTLLFLPFVDPFVRLVKSIIPDDEGMVLMGPRFLDRNLIKASPIAAVDAV
                     RKELVRMGLLTLDMLRDVKTAFIDDDRRMIEQVHQTEKVVNELNREVASYSAELMQVS
                     LPPVLSSSLSSYVNGVGDIERVGDHAENLIELYEYKVEHNLPFSDVAMEEFEVMIDLS
                     EKTARLAVEALDTADGEKARQVFALEDEIDAMERGLRRRHIDRLNQGVCNPGSGVIFI
                     DILSNLERVGDHANNIALIVKDINLVRAKEA"
     gene            complement(14246..15958)
                     /locus_tag="PLM30_00415"
     CDS             complement(14246..15958)
                     /locus_tag="PLM30_00415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOR53663.1"
                     /translation="MSSKAPRGKRGRKSPARDELRFIPLGGLGQIGKNMFVLEYGEDI
                     IVVDCGLMFPDEEMLGIDFVIPDASYLFENRERIRGLVLTHGHEDHIGSLPFILPRID
                     VPLFGTKLTLAMVDAKFQDSCPDYKPRSVEVQAGEKFTLGCFGVTFLSVCHSIPDGVG
                     LAVETPVGTVIHTGDFKLDPTPVDGRITDYSAFADFGSKGVLLMLSDSTNVERPGFTQ
                     SERIIGRTLERLLQLHRNKRVIIAAFSTNLHRSQQILQAASRFNRKVALMGRSMVQNV
                     NLAMDLGYIDADRDILVPMEDAGRVPDNKLIVLTTGSQGEPFSGLVRMSKGEHRFVNL
                     GPGDLVAVFATPIPGNERLVSRTINRLFECGCEVVYDRDQEVHVSGHAAREELKMMLS
                     IVRPRYFVPLHGEYRHLVRHAKLAQEMDIPQKNTFVLQNGDVLGLDGNRAQVRRGAAP
                     SGDILVDGMVLGEMQNSLLKERLEISEEGIVVVSLVLDGELRLAAGPIIESIGFLHMD
                     DAQQIKGDLVREIESTLEKHSGRTGEEEQRVAQKVRGRVRNVLKKHLRTPPVVRVLIT
                     VIEG"
     gene            complement(16078..17346)
                     /locus_tag="PLM30_00420"
     CDS             complement(16078..17346)
                     /locus_tag="PLM30_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOR53664.1"
                     /translation="MDQSSRSFRELLEKRPLNVQAVFGGEPVALVSGRDIAAAARRTG
                     SIVLAANVRNPLTIKGVLMAARDLNAFVLLELAKSESTYCGCTFENVPQLALQYSSEL
                     GGGVPFGLHVDHYAIKSREDLLKSIPHLRKLVESGWTSVAIDASHNPDWENLCFTRDV
                     AMHVPAYLGLEVEVGEIKGPGELTTVEEALFFIGGLNSWRVFPDFLAISNGSKHGTYD
                     STAGEAEGIDLKRTGEIAEAIAPYGATIAQHGISGTPLDKVSRFSKFGINKGNVGTLW
                     QNIVFGLEMDPETGNAVVKDGTYIKDPGRGIPGELWDDMVRWADGQGYSRKSGDYKKA
                     NKPFHDAIMALPERIQARIVDETAEWARRFIKAFHAEDGADALLEVISRRTDHNAAPE
                     QAVVASRKDFTEAASPDRAGAGEKAQDHSD"
     gene            17505..17579
                     /locus_tag="PLM30_00425"
     tRNA            17505..17579
                     /locus_tag="PLM30_00425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17538..17540,aa:Gln,seq:ctg)
     gene            17592..17667
                     /locus_tag="PLM30_00430"
     tRNA            17592..17667
                     /locus_tag="PLM30_00430"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17626..17628,aa:Glu,seq:ctc)
     gene            complement(17710..18885)
                     /locus_tag="PLM30_00435"
     CDS             complement(17710..18885)
                     /locus_tag="PLM30_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOR53665.1"
                     /translation="MIELVIHARDVDEPTMAQIRDIAAHPAVDGLVAIMPDCHLGAGC
                     VIGFTGKFKGAVIPNVVGVDIGCGVLLAPIEGVKKGDIDFTALDGFVRNSIPLGKTRH
                     RGPAMLDWFDARFPGRGARARELGELADEGFYGKLLARRPKGPAWSQLGTLGGGNHFI
                     EINEGLDGALFLAVHTGSRHFGFQVAGFFQGLARRYQSKAGASSGLPRGLEHLPLDKG
                     GSEYMKWLRIAQEYAGLNRFIIADRILSFFGLRLNEGTLVESVHNYISEKDGIVRKGA
                     ISAHKGERLVIPLNMASGIVLGVGKGNPDYNHSAPHGAGRLFSRKEMKRRLKEGSITM
                     GSFRKSMEGVFSTSVLKETIDESPMAYKRWEDIRDEVGEAVEVSALLRPVYNLKAPD"
     gene            18970..19785
                     /gene="nudC"
                     /locus_tag="PLM30_00440"
     CDS             18970..19785
                     /gene="nudC"
                     /locus_tag="PLM30_00440"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOR53666.1"
                     /translation="MEDYMDPEELLTAPSATRAPCFAFRGDLLLLRTEPGVELPVWED
                     IAGLPLSGRSPGFWITGRPGNCWASLVEDAPLPEGMSLVTLRDVWSLMGERAFFEAGR
                     AFQIMEWSQRNRFCGRCATPMVEAENETALVCPACGLVSYPTVTPAVIVAVEREGRLL
                     LARNTRFPKGRYSVIAGFVEPGETLEGTVEREVMEEVSVRVKNITYFGSQPWPFPHSM
                     MVGFRAEWESGEIMADGNEIESAGWYSPEGFPDIPPGFSISRRLIDDFLARHA"
     gene            complement(19809..20228)
                     /gene="speD"
                     /locus_tag="PLM30_00445"
     CDS             complement(19809..20228)
                     /gene="speD"
                     /locus_tag="PLM30_00445"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOR53667.1"
                     /translation="MDPRGIHYIVEASGCSEVIGEVDRMQEILVEAARKANTHVWAVS
                     FHRFPPNGVSGVVVISESHLSVHTWPEARYLALDIYTCGAESIPEQAVSHVLERVQAG
                     HTHISELTRGLDDDGDRIYYHSLITWEEELRKGKPKN"
     gene            complement(20275..>21261)
                     /gene="glyA"
                     /locus_tag="PLM30_00450"
     CDS             complement(20275..>21261)
                     /gene="glyA"
                     /locus_tag="PLM30_00450"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR53668.1"
                     /translation="HSGTQANMGVYFTVLEPGDSILAMDLAQGGHLSHGHPLNFSGRL
                     FKIIPYGVDRERETIDYDEVARLAREHRPKMIVAGASAYPRLIDFKRFGDIAKEVGAY
                     LMADMAHIAGLVAGGVHPSPVPHSDFVTTTTHKTLRGPRGALILCRGKYAPGLDRNVF
                     PGMQGGPFMHVIAGKATCFLMAMRPEFKEYAAQIVKNAKALAGELGARGYRLVAGGTD
                     NHMLLLDLRSKGLTGKEATDILGRVGITVNKNMIPFDPEKPMVTSGIRLGTPALTTRG
                     MKEKEMALVAGIIDVALSESQDRSAMDRVAVKVRDLAGSFPLYPELKKGYYF"
BASE COUNT         4140 a         6830 c         6175 g         4116 t
ORIGIN      
        1 gaaaagcgtt ccggccccta ggaagacctc ccgggcactg cctgaagggt tgaaaaaaag
       61 gtccgcttcc cctggcttaa gggagcggac cgttagtgac ccctcatcga agaaattttc
      121 aaaggcccat cacgatggtc tgctccctgt tcggcccaac ccccaccaac acaatgggga
      181 ccccggtctg ctcctcgatg aaacggatgt aatccctcgc ggggcgggga aggtcttgga
      241 aatcccggca ggccccaagg tccattttcc agccctccat gttccggtag accggttcga
      301 ctgagtcgag gaagaccgag ttccccttgt agggcaggtc ttcaccctcg tcggacctgt
      361 aaccggtgca gacggggatg gcctcgaacc ccgaaaggac gtccagtttc gtcaacgcca
      421 gggcgtggat gccattgatc ctgacggcgt acttcagcgc cacaaggtca agccacccgc
      481 agcgcctggg ccgccccgtg gtggcgccga actctccgcc cctttcgcgc agggtttcac
      541 ccagttcttc cttgagttcc gtcgggaagg gcccctcgcc gaccctggta ttgtaggcct
      601 tgaccacgcc ggtgacgcgc cccaccaggg aaggccccag tcccgccccc acgcaggccc
      661 ccccggccac cgggctggaa ctcgtcacaa aggggtaggt tccgtgatcc aggtccagga
      721 gggtgccctg ggcgccctcg aagagaacgt tgctccccct ctccagggcc tcgaagatgg
      781 tcagggaagc atcgcccacg taaggtttaa gtctctcgcc ccacgaccaa gcctgttcga
      841 agatctcccc gaaggccagg ggctcttccc cgtagatcct ggtgaggtac atattcttga
      901 tctccaggtt gaaggacagc ttctctctca gcgcgtcctc gcaaagcagg tcctcgaccc
      961 ttatgccgca gcggttgacc ttgtccatgt aacaggggcc tatacccctg tgggtggtac
     1021 cgatcttctt cctgctcccc ctcaaccttt cctccatact gtccagtttt ttatggtagg
     1081 gcatcacgac atgggctgcc ccgcttatca ccaggcgagc cctgtccttg cccctttcct
     1141 gcaggtcgct gagctcatcc aggagctgct gcgaatctat caccacgccg ttgccgatga
     1201 cgcaggtctt acctgaatag agcatccccg agggaaggag atggaagaca tacttttccc
     1261 cttcgaccat caccgtgtgc cccgcgttgg cccctccctg gtaacgggcc accacgtcca
     1321 cggtgtgggc catcgcgtcg acgaccctgc cctttccctc gtccccccat tgggtgccga
     1381 cgattatctc cactcgacct ttcatagggt cattcctccc agccgcccat actttttcgc
     1441 gccaggtatt cgggcacggt cacgaattct acatcctcgg gggtcatttt ccgcaacttc
     1501 gggagaaaat ccagggtccc cggcctggca tggcaaatag ccacggcccc cccgttcttc
     1561 cgggcccggg cgaaggcttt tcggaactgc tcccggagga aactctcctc gtcgtaatgg
     1621 tccaggaaaa agcggttcga ggtggccggg agccctgtct ccaccgcgac atggtaggcc
     1681 accgatcccc cggaggtcct gctgtcaacg aagaagagcc cctcggcctt cagttcgagc
     1741 atgaccccgc ccatggcccg ggggtcctcg gtcgccctgg aaccctggtg attgttaagg
     1801 cccgctgccc ccggcagcga ccagagggcg ttcctcacgg ccacccttac ggcctcggga
     1861 tccatccccg ccctcaccag ggctttgcgg tccccgggac tgcccttggc ctccatgggc
     1921 atgtgtacca ggaaggggat gccgagatcc ccggccattc gagctgtcgc ttggctgaaa
     1981 cgttgatagg gtatgatcgc ccaggtcacc gggatcggga tggaggccag ccgtttcgcc
     2041 gcgtccaggg aaaaacccag gtcgtccaca atgaccgcga cggcggggga ctggacgaac
     2101 cccccgtagc accggtcagc tcccgggaag accagggcgc agagggcgac aaaaaggaac
     2161 ctgctagcgg ctttcttcct ttttcccatc tatcaggccc tggagctcca gtatcgccct
     2221 cttcatctga gcgtcgctct ccctgtcctt tacgaattcg ccctccacct ctatgtcggg
     2281 tgaaaggccg accttgtcga tcacggtgcc cgcgggggtg taatacttcg ctatggtgac
     2341 gaagatcccc gacgagtctg aaaggttgaa gagggtttgc accgacccct tcccgaagga
     2401 ttgtttgccc acgatcacgg ccctcccatg gtccttgagc gccccggcga ggatctcgga
     2461 ggcgctggcg ctgccttcgt tgatcagcac ggccatgggg aggtcggtta tggtcccctt
     2521 cctggcgtag tacacttcgt tggcgcggtc gaaacgtccc cgggtgctta cgatgacccc
     2581 gtcatcgagc aggaggtccg ctatttcaac acaactgttc aggagtccac ccgggttgtt
     2641 cctcaggtcg atcaggaagg ctccggttcc ctctccggtg aggtcatgaa cggcctttcg
     2701 aaaatcgggc agggtcttaa ggttgaactg ggttatcctc actagaccga tccggtcctg
     2761 caacagctcc gacttcacgg tctggatctt gatgctttcc ctttcgatct cgaacttgag
     2821 cagttcatcc tcggactcgc gcctgaccca gatgaccacc ttcgtccccg gttcgcccct
     2881 taaggcgtgg accacttcgt tctggggcca accaaggata acctcatcat cgatctttac
     2941 gatctggtcc tttggtttaa ggccgaccct gtcagccggc gtgtcctcta tggggctaac
     3001 aaccagggtc ttcccgtcgc gctggccgat gtagatcccc aggccgccgt actcaccctg
     3061 gagttcgatc tcctcttcct tgagttcgtc cgggtcgaca aaacgggtgt aggggtcctt
     3121 cagcgacgag accatgccct tgatcgcgcc gtggaggagt tccgtttcgg agatgtgttc
     3181 ctcgccgtcg acgtggtagg tctcgaggat gacgcgggcc tgcttcatga gccacaggtt
     3241 ctggaagctg aaaggagcgg acttgaagag gtccatctcg ttgaaggcga cggccgacag
     3301 tactcctccc acgaggagag cgcccactac ggcccctatg aaaacatccc ttgcccgacg
     3361 aaacattttt cctattccac ccttcagact gcactacctg ccgaggtagg atacagggtt
     3421 cttcgcctct ccgttgaccc tgacctcgaa atgaaggtgg ggccccgatg ccaccccggt
     3481 gtttcccacg ttccctatga cctccccgcc cttgacaacc ttaccctctt cgacacggat
     3541 ctgggagaga tgagcgtaga cagtggagag gtccccaccg tggtcgagta tgacgatctg
     3601 gccgtaaccc cggagccagc cggcgaagag tacctccccc gcggccgcag ccttgaccgg
     3661 ggtgcccgcc ggtgccctta tgtcaaggcc cgtatgcatt atcttggtct tgaacacggg
     3721 gtgcaccctc gtgccgaatt ggctgcttat ctcacccctg atcggccagg aaagcttcgc
     3781 ccccgaaggc aagtataccg gcgggacgcc tgcccttgac tcctgcatct tccttttctg
     3841 ggtcatgagt tcacggatct tgccctggag ctccttctgg gattcttcca gttcccgggc
     3901 ggccttctgg tgcagggcct tttgcttcgt caaggtgtcc agggactgtt ttctttgcgc
     3961 ctcagagttc ttcaaggctt tccggtcatt ctccaggctt tccttctgct tgaccagcag
     4021 ggccttcttg tcaagcatct gcttcctgga ggattccagc ttcattttac tctccttcat
     4081 ggtcactatc ctgcgttcat cctcccgggc catcctggcc aggagatagg cgttggtgag
     4141 agcctcatgg gtactgctcg acgagagcag gaggttgaac tccgcgacgc ccccgtactt
     4201 gtagatcgct tcgaacctcc tggcaaggta acccctggtc ttggccaggt ccgcctcggt
     4261 gctctggatc tcggcggtga gctccctgat gcccagttcc gtcttctcgc gctgcaggtc
     4321 gagaagggcc actttctgct tcagcagggt gacgctctgg tcgatccggg aaagatcgtc
     4381 aagcaacccc tgctccttcc tccgtgcctg ctggatctgc ttctggtgga aggagatctg
     4441 cttttctatc gcttccaggc gaccctgttc atccttgatc ttcgagtcga tatcaggtgg
     4501 gttggccccc caggctaccg ccggcgagag ggctaaaaag agcaacagaa ttaccgtggt
     4561 cgaaaaccag ccttttccct tcatgatcct tgcaccccgg tttccagcta catggacctg
     4621 gacgcccgtc tcacaaaacg ggacgcggca agccagctgc agatccagcc gacagacagg
     4681 cccgccagga ccagaacggc gccgaccctg aggacgatga gtctgtcggt gagcaacctc
     4741 aggaaaggaa gggacctttc cagggtggta agggccatac cgtaggagaa ccataaaagc
     4801 aggatggaga gtgccgcacc cagggtaccg agaaggactc cttgcaggac atagggaaag
     4861 atcacgaaac tcctggaggc gccgacgagg agcattttgc ttatctcgtc cttcctggcg
     4921 tagacgccga tgcggatcgt gttgaacagc accagggccg ccgaggccag ggagaccacg
     4981 agcacgacca tggacagcct ggatacgaaa cgggagatct tttgcagcct ctcagcgata
     5041 tcccccgcgt agaccacttc ttccacctcg ggcatggcga ccagttcccg ggcgatgacc
     5101 gtcgcgaagg acgccttttt cacctgtacc tccacgctgg gggggagggg gttttcctct
     5161 agcagggcca ccgcttcggc aagtttgcca agccttgcct tgaggcgttc cagggcttcc
     5221 ttggcggtaa cgaccttgac cgatgagacc gaatcgtagg aaatggcctt ccgtgccacc
     5281 gtttccaggg cctcctccga attctggaca taagcctgga tcgtaaggtc gccttcgacc
     5341 gtctcgacca gataccgggt gttgaggaca aagaggatgc tggcacccac gaggaagaag
     5401 accgccacag atgtgacgat cgtcaggaag ctcaacccgg aatgcctgga gaggagcctc
     5461 caggtatccc tgatcatgta cctaaaggtc cccatcggat cggtacctgc ccttcctctc
     5521 gtccctgatc agccgcccct tgcggagctc tatgaccctt tggcgatagg aatcgacgat
     5581 gtactggttg tgagtcgcca ccaccagcgt ggtcccggag gcgttgatcg acagcaggag
     5641 ctggaggatc tcgtcggcag tatccgtgtc gaggttgccc gtgggctcat cagccaggag
     5701 gatcgaaggg gaattcgcga tggccctccc tatggccacc ctctgctgtt caccgccgga
     5761 gatctgctcc ggaaaaaggt tccttttcct ccaaaggccg agacggtcga gtacttcgtt
     5821 ggtcctcttc tgcacgcttt gaaaaggtac cccgatcact tcctgcacga aggcgatgtt
     5881 ctcatgaacc gtcaggtccg gtaggagccg gaaatcctgg aagaccaccc cgatattcct
     5941 cctgagatag gccaggttgt gggaggctat cttccttata ttgacgtcgc ccacggtaac
     6001 ccgacccttg gtgggcacca gttcccgggt gacaagcctg agaagggtcg tcttgccgga
     6061 tcccgtgggc cccacaagat aaacaaactc ccccttgtcg atggagagga aaatatcctg
     6121 gagggcgacg atatccgggg ggaactgttt ggtcacaccg gcgaaccgta tctccattca
     6181 ttacctcctg cgcatggacc aaacctgggc cgactccccc acgatctccc tgaaggtcaa
     6241 cccgtagtac tcctgcagtt ccaggggggt tccgctctgt ccgtatcggt cgcttacggc
     6301 gacgctcttc acgggaacgg ggttttccga ggctgaaagt gcggcgatgg cctcgcccag
     6361 ccccccgatc acgttatgct cctccgccac gacacagcac cctgtccttc tcagcgacga
     6421 gaggatcaaa cgggagggca ggggtttaag gcagaaacag tcgatcactt cggcgtttat
     6481 gccttgctgg gagaggatcc tggcggcctt caacgcttcg tggaccatga taccacaggc
     6541 gcatagggtg acgccgtccc ccaccaacag gagcctccca ccgccgatgc gcatttcggc
     6601 ctcatcgaag gggcccgtgt cggggacctc gtcacggccc agccggacat aaacgggatc
     6661 cgggtgcccg atggccagcc ccgccatcgc gaaagaggaa tcgaagtccg aaggcacgag
     6721 gacggacatc ccgggcaggc ttctcatcag tgccgcatcc tggttcatct ggtgcacggc
     6781 cccgtcctct ccggcggaga ggcccgaatg ggtcccgacc aggacgaccc caagatccgg
     6841 gatcgccagg caggtcctga cctggtcgaa agacctcgcg gtgaggaagg ccgacggggc
     6901 gatggcgacg ggtttcttgc cctccagggc aaggcccgcc gcggtcgtca ccatgtcctg
     6961 ttctgaagct ccgcagttga ccgtcctctc gggaaaaagc gaggcaaagg gggtgacgat
     7021 ctcgctcccg gccatgtccg cgtggaggag caccacgtcg cccctccccc ggccgagctt
     7081 cataagtccc tggccgaaac cggaaagggt gctcctcatg gtttcacccc cccttcgatc
     7141 tcatccgaag ccagctccag ctccctgagc gcctgttcca tcccgttccg gtccaggggg
     7201 cggcccggct tctccaatgg ccccttttct gcggaagaga cgcccttgcc aatgatggtc
     7261 ctcgccatta tcacggaggg gagcccttcg gggcccgggt gagccctcaa aactgccctt
     7321 tccaggtgat cgaaattatg gccatcggct tccatcaccc tccagccgaa ggcttcgaac
     7381 cttttcctct ccgaggctcc cttccccggg gccagggttt gcgctgccga tggcggggcg
     7441 ctggaatcca ccactaccgt caagccttcg taaccaaggt cgcccgcccg gagggccgat
     7501 tcccacaggg atccctcccg tagttcctcg gaaccgatga cgcagaagac cctcctgtcg
     7561 ttgccatcca tccgggatga aagcaacagt ccattggcga tgcctatggc catccccctc
     7621 gaccctccgg gggcgtcgac accgggcgtc ctggcgtgct caggcctacc ctggagcatc
     7681 gcccccaggc gtcggtaatt ccagagttcc ttcctttcaa agaaaccgcg ccgggcaagg
     7741 accgcgtaaa gggcggggct cccgtggtcc ttgctgaaga cgagcctgtc cctctccggc
     7801 cagagaggtt catcgggtct tatcttcagt accttccagt agagatatac caggatgtcc
     7861 acgacggata gcgaggaatc caggtggcag gccctcgaaa cgccggtcat cctgacaata
     7921 tctttcctca cctccagggc cgtcctttcc agttcccgtc tttgtgaggc tttcattgca
     7981 tcactctcct gagagcattc cttcaaggac acggtggaaa acctcctcca gcgcgcccct
     8041 tgcttcgtcg agcctggcat cgtgccggag ttccagcggg aaaggaggaa gtcccacccc
     8101 atcccacagg ggtaggcccc agtcctccgc gaaggacctg accttggggt catagggtac
     8161 cgccaggcac ggcacgccgg caaggaccga gatgaggcaa aaatggagcc tcatgcccac
     8221 ggcagctctc gctttcgacc atagatccct cgcctcggaa gcgtcaacta cgcggacaac
     8281 ccgggagaga gtgagcgccc ctcgggatcg gagatcctcc atgagggact cgtcctcgcc
     8341 ggaaagtgcc accccgatcg gatcaagccc tgatgtttcc ccggcccggg tcgccgccag
     8401 ggaggcggct tccggaagcc cacctcgcca gggcctgaca ttcacgagga gaaggtcccc
     8461 actgccgctt gaaaaaccgg gggccatcga gaaaacgggg tcaggggcga tctcaccggc
     8521 caggtcccac tcttccagga gcgacatcga ccccctgtcc cttaccaccc tcgcccggca
     8581 cagcgaaagg gcgtcgcggg ccaggaagcg ggccatgccc ctctccaggg gacctatcga
     8641 ctggccgaag gcccagggcc tgcagccggc caggcgggcc atcctggcca ccccccagta
     8701 ataggcgcag gacctcgtgc tggtggaatc ctgaaagaga ccgcccccac cgaggagaag
     8761 cgtcctgctg gacctcaagg cggacatgac ctccaggggg ctccagcggt cgacccggga
     8821 agcctctccc cggcctgccg tgaccccttg ggcggccgaa agcaccgcta ccctttcgcc
     8881 aggcacgccg ttcttgttca aaaggcccaa aaggctttca gccatcagtt cgtcgccgag
     8941 gttcccgaaa ccgtaataac cacaaaggac gacgtccagc ggccggctca ccccgcgaca
     9001 accccctgcc accttctcca ggccggtaag accaggaaca caaggaccgc cgccataatg
     9061 gagcccacga tgaggccggt ccagagcccg ttaaaaacgc gcagaagtat aaataaaagg
     9121 tgtgtgtgga aatggcagaa gctgttcacc accgacgaaa aggccagcgt ggcgccaagc
     9181 cggaatacct cgcgatattc cctccagatg tcctttcttc tcacgacgaa ccagagcacc
     9241 agtgccgggt agcctgcgaa gatctccttg gtcctgggcc gtgctaccag caattgctcc
     9301 aggaactccc tgaacctgat ctcccagcct ggaacgaagg atacatttcc gctcctgagg
     9361 gccatgacgc cggcggcggc ggccagtatg cccacgaggg ccaattcacc ccagacaggc
     9421 ggtctcgaca tcacctgggt cagcgattcg gggtgaactc tcctcttcag gtcgtgaagg
     9481 agcaggatca gcggtgggag caggagcgcg atcttgacac cggagaaggt gcccatcctc
     9541 agcatgtacc agggggtccc gaaaaaggaa gcgatggaca ggccggagag gacggcgacg
     9601 gccacgccgc agatcactcc ccgaaggggg cgtctgaagt cccccagggc aaccagggag
     9661 gcctcggtcg cggcgaggga agcgaccagg gccccggaga cccgcgcaac gaccgggacc
     9721 ttcagggaga ggaaggcaat ggccatcgaa agggcaagcg aggccaccgc ttccttaagg
     9781 ctgatgtcgc ccacctccat cgaaaggaac ctcgctgtca gtctcaccgc aagcgctacc
     9841 aggacaaacc cgagagcaag ggccgcgaag gggctcgtgc cccagcgacc catcgtatcg
     9901 ggccagccgg tgctgatccc cagcgagctc aatcgcttcg atatcatggc gcactcgtcg
     9961 ccgaacctgt tcagcctgga gccggagtgg aggtccgagg gtctcaggta aaggatcttg
    10021 acggagcgtt cacgggaggc ccggacaaat ctctccacca ggtcgtcccg ggtgagcctc
    10081 ctgctgagga tctcctccct cctcacgctg tgcagcggga ggatgtccgg gaaaaggccc
    10141 ctctcgagga gagcggcgcc tatctgctgg gagaactcca cctttgagac ggggacgccc
    10201 cgctttcgga gaacctccac aagaggggcc aaatccgggt aaccggccac gaaaagtcct
    10261 gccgggacaa tggctcggat ctcgggaaag gcatccagta ccgaggctac cgcgagggcc
    10321 acgtcttcgc cgccgacgcc tggggtcgac cccgggcgaa agaccagagg gatacctctc
    10381 cccttgagca actccagcat ggggtagtcc gggaacacgc cggcctcgag ggtctcggcc
    10441 agggaaaggg gtagcaccag cagttctccc cggtccaggc ctatggcccg gctcccggga
    10501 tactttttaa ggacgaaagc cctgatctca ctggaaaaag gagaccccgg ccgaaggagg
    10561 acggccgccg aatcagggaa caggccctcg ggcctcgggc ccgggaggag ttcccccacg
    10621 gacccgtatc ccaggtccag cgctcccgcc atgagttcct ggcccgtcag ctcccccacg
    10681 gccaccgcgg tgagtccccg tccaagcagc acatcaagga cctccgggac ggcaagttcc
    10741 gactccctgg caaggcttgc gacatccctc atatcggtga ccagggccgc cacattgccc
    10801 tgcctttcgg ccctggcacg agggagaagc cccgcgaccg ctaaaagggc ggcaagaacc
    10861 agtaccgcta tgaaaaagaa ggatcgccca ttcctcacac tctttcaccc cactgatcct
    10921 cgagggggat ccccataccc tccagcagcc tgctcaatgt gaatagggga agtcccacga
    10981 cattaaaata acagcccctt atggagcgga caagcaggga accccatccc tggatggcgt
    11041 aggccccggc cttgtccccg ctctcattcc ctgacaggta agccccgatc tgccggtccg
    11101 taaggtccct gaaggtgacc agggtctctt cgatcgccga cgccgacttg tccccccacc
    11161 gtacggcgac gcccgtgatg accttgtggg ttttcccgga gaggaggcgg agcatccgga
    11221 aggcatcgcc gggatgatcg ggtttcccaa ggatcatggc cccaagtacg actatcgtgt
    11281 cagccgctac cacgagggaa ctccgctggg aagcgaaaac ggagtcagcc ttcgcctccg
    11341 ccagcctacg cgcctgctca ccgggatcac cgtcttccaa gatgctctcc tcgacagcgg
    11401 gcggtacgac cctgaagcgc caccccaggt cacgaaggag cgcctcccgc cttgggctgg
    11461 ccgaagccag gaccatgtct gggaaaaggc tggagccatg cccggtcatc gaagcaccag
    11521 caccccgaag atcccaccga tcaaggaccc gaggttcgca cggatataaa gccgaaagcc
    11581 gagatctatc acgatgaggt cgaggttttt catgttgaag ccgacatcta tgatattacg
    11641 gaaaagcctc tcggtggcgg taaacccctg caaaaagacc cctaaaaagc tgccgaggat
    11701 cacgcaaagg atcacgaccc accatccctt gtaggagact ttcgccatca tcttcacccc
    11761 ctgatcagtt tgaggcccac cacgagaaga cccgctatca tgcagtaaag ggaaaagaaa
    11821 cccaaccccc ccctgacgac aaccctgcgg agaacgctta aggctaaaag ccccgacccg
    11881 aaggcgaccg ccacccctat ccaccacccc ttcggcagcg acgtgagcac gccggacagg
    11941 cctcccgcct cggtcacctc gaggatggcc gcgccgagga tcgctgggag ggacaggaga
    12001 aaggaaaagg agaaggcgtc ttcacgggaa agccccgccc ggagtcctgt cacgatggtc
    12061 gccccggacc tggagatgcc cgggaaaacc gccagaccct gggctaggcc gaccttgagg
    12121 cctgaagagg gcgagacggg aaggaccccg ggggaaattc ggctcccgta gcgcaggatg
    12181 aaccctgtca ccaggagggc gaagcccacg aaaagggggg aggagaagag gatatccacc
    12241 accggcttga cgagaaggac cactagggcg gtaatcagcg tgcccgcgat gacgccccag
    12301 ccgtacttcc agccgggaag cccgcgttcc ttcactgaaa agaggccctg gaaccactcc
    12361 tttaggtttc tcattatgtc aaaccaaaag aaaaggaccg tggcggccat ggtggccagg
    12421 tggagcacta cggcgaaggg aagggccggg cccgaccagc cgaaaatatc ctgggcgatc
    12481 acgaggtgac cggagctgct cacggggaga aattcggtga taccctggac aaggcccaga
    12541 acggcggatt gaaggtacaa gggtcatgcc tccttggcgc ggacgaggtt tatatccttt
    12601 actatcaggg ctatgttgtt ggcgtgatcg cccacccgtt caaggttgct caggatatct
    12661 atgaagatca ctcccgaacc cgggttgcat actccctggt tcagcctgtc gatgtggcgc
    12721 ctcctcaagc ctctctccat cgcgtcgatc tcgtcctcca gggcgaagac ctggcgggcc
    12781 ttctcgccgt cggccgtatc gagggcttcc accgcgagcc tcgcggtctt ctcggaaagg
    12841 tcaatcataa cttcgaattc ctccatggcg acatcggaga agggaaggtt atgctctacc
    12901 ttgtattcat agagttctat caggttctcg gcgtgatccc ccaccctttc tatatcaccc
    12961 acaccgttca cgtaactgga caatgacgat gagagcaccg gggggaggct gacctgcatc
    13021 aattcagccg agtaggacgc gacttccctg ttcaactcgt tcacgacctt ctcggtctgg
    13081 tggacctgtt cgatcatcct ccggtcatcg tcgatgaagg cggtcttaac gtccctcagc
    13141 atatccaggg tgagcagccc catcctgacg agttccttac gcactgcgtc aacggcggct
    13201 atgggcgatg ccttgataag gttgcggtcc aggaaccttg ggcccatcag caccatcccc
    13261 tcgtcatcgg ggatgatgga cttcaccagt cgcacgaagg ggtccacgaa ggggaggaac
    13321 aacagggtgt tgaggacgtt gaaaagggtg tgggcgttgg ccagctggcg ggctatatcc
    13381 gaggaggtga gcaacacgag gtccttaaag ggcccgacaa gggcaaggaa tatggccacc
    13441 ccgatgacct tgaagagaac gtgggcaagg gccgactgtc tcgccgatcg gttcatcccc
    13501 agcgaggcca atacagccgt gatcgtggtc ccgatgttgt ccccgaacag gatgggtatg
    13561 gccgagtcca gggtgagaag cccttgggac cccatgacga tggtcaggcc gaccgtggcg
    13621 gaactggact ggaccagcat ggtgagggcc gtccccgcca gcactcccag gatggggtga
    13681 tgctggaaag cgaggtagat atcctggcgc tccctcagga aattcatggc gttccccatg
    13741 gtcaacatcc ccaggaagag gaggccgaaa ccgactatcc cgttgccgat gaacttctgt
    13801 ctcttactcc tcccgaacac ggcgaagagc atcccgaacc ctatcagggg gagggcgaaa
    13861 tcccctatct tgaaggctac cagttgagcc gttatggtgg tgccgatcgc cgcccccatc
    13921 atgaccccca cggactgctt cagcgtcatg aggccggcat gaacaaagct cgcggtcatc
    13981 accgtcgtgg ccccgctggc ctggatcacg gccgtgatca gggcccccac ggctaccccc
    14041 ttcaaggggg aactggtcag ggccgagatc aggcgcctca tcctgtcgcc cgcgaggtcc
    14101 tggagcgact cccccatcag ctttatgccg tacaggaaga gccccacccc accgaggagg
    14161 ttgaaaaaaa gccccgtcga caaaccctca ccaccctttc caggtaaaaa aaccgcaggg
    14221 gaaccgcctc aagaccaaga caggcctacc cctcgatgac cgtgatgagg accctcacca
    14281 ccggcggagt cctcaggtgc tttttcaaca cgttccggac cctgcccctc accttctggg
    14341 cgacccgttg ctcttcctcg cccgtcctgc ccgaatgttt ttcaagagtg ctttcgatct
    14401 cacggacaag gtcgcccttt atctgctggg cgtcgtccat gtgaaggaac cctatgctct
    14461 cgataatggg gccggctgcg aggcggagct caccgtcaag gacaagcgac accaccacta
    14521 taccttcctc ggagatctcc agcctctcct tgaggaggct gttctgcatc tcgcccagga
    14581 ccatgccgtc gaccaggata tcacccgacg gggcggcccc ccttcgcacc tgggccctgt
    14641 tgccgtcgag gccgagcaca tcgccgttct ggagcacaaa ggtgttcttc tgcggtatat
    14701 ccatctcctg ggccagcttg gcgtgcctga caaggtgccg gtactcaccg tggaggggga
    14761 cgaagtaccg gggcctcacg atgctgagca tcattttaag ctcttccctc gccgcgtggc
    14821 ccgagacatg gacctcctgg tcgcggtcgt agaccacctc gcacccgcat tcgaaaaggc
    14881 ggttgatcgt cctgctgacg agccgttcat ttccggggat gggcgtggca aagaccgcca
    14941 ccaggtcgcc ggggccgagg ttgacgaaac ggtgctcacc cttgctcatc ctcacgaggc
    15001 ccgagaaggg ttctccctgg ctgccggtgg tcaggacgat cagcttgttg tcgggcactc
    15061 ggcccgcgtc ctccatgggg acgaggatgt ccctgtcggc atcgatgtag ccaaggtcca
    15121 tggcgaggtt cacattctgg accatgctcc ttcccatcag ggccaccttc ctgttgaacc
    15181 tggacgcggc ctgcagtatc tgctgggacc tgtgaagatt ggtggaaaag gcggctatta
    15241 tgaccctttt attccggtgg agctgtaaaa gcctctccag ggttctcccg atgatcctct
    15301 ccgactgggt aaagcccggc ctttccacgt tcgtcgagtc ggaaagcatc aacagtacac
    15361 ccttgctgcc gaaatcggcg aaggccgaat agtccgttat ccttccatct accggggtgg
    15421 ggtccagttt gaagtcaccc gtatggatga ccgtcccgac gggagtctcc acggcaaggc
    15481 ccactccgtc ggggatcgag tggcagaccg acaagaaggt caccccgaag cacccgaggg
    15541 tgaatttctc ccccgcctgg acctctaccg accggggctt gtaatccggg caggaatcct
    15601 ggaacttggc atccaccatg gccagcgtga gcttcgtccc gaaaaggggc acatctatcc
    15661 tggggaggat gaagggcagg ctgccgatat ggtcttcgtg gccgtgggtg agcacaagac
    15721 cccggatcct ctcccggttt tcgaaaaggt aggaggcgtc agggatcacg aaatctatgc
    15781 ccagcatctc ctcgtcgggg aacatgaggc cgcagtccac gacaataatg tcctcgccgt
    15841 attcgaggac gaacatattc ttgcctatct ggcccaggcc ccccaggggt atgaagcgga
    15901 gttcatccct ggcggggctt ttcctccccc gtttccctct cggtgctttt gacgacatcg
    15961 acacacctcc ggcttcaccg ctttgaacct tcagcggcca atggcaaggc atagcaagac
    16021 ttcccgggtc aaggcaggca tccgggaaag ttccagatgc ggccggcccg cccggcttca
    16081 atcgctgtga tcctgggcct tctcacccgc ccccgcgcgg tcgggggaag cggcttccgt
    16141 aaaatccttc ctggaagcga cgaccgcctg ctcaggagcg gcgttatgat cggttcgtct
    16201 tgaaatgacc tccagcaacg catcggcgcc gtcctcggcg tggaaggcct tgatgaacct
    16261 cctcgcccat tccgcggtct cgtcgactat cctcgcctgg atcctctcgg gaagggccat
    16321 gatggcatcg tggaaaggct tgttggcttt cttgtagtca ccggacttac gggaataccc
    16381 ctgaccgtcg gcccaccgca ccatgtcgtc ccaaagttcc ccggggatgc ccctgccggg
    16441 gtccttgatg tacgtgccgt ccttgacgac ggcgttgccc gtttcggggt ccatctccag
    16501 gccgaagacg atattctgcc agagggtgcc cacgttgccc ttgttgatcc cgaacttgga
    16561 aaaccgggac accttgtcga ggggggtgcc ggagatcccg tgctgggcaa tggtcgcgcc
    16621 atagggcgcg atggcctcgg cgatctcccc cgtccgcttg aggtcgatcc cctcggcctc
    16681 cccggcggtg ctgtcatagg tcccatgctt gctcccgttg gagatggcca aaaagtcggg
    16741 gaagaccctc caggagttca gcccaccgat gaagaacagg gcctcctcca ccgtggtcag
    16801 ttcgccggga cccttgatct ccccgacctc gacctccagt cccaggtagg ccgggacgtg
    16861 catcgccacg tccctggtga agcaaaggtt ttcccagtcc gggttgtggg aggcatcgat
    16921 ggccaccgat gtccaccccg actcgaccag tttcctcagg tgagggatgg acttcaggag
    16981 gtcctccctg gacttgatgg cgtaatggtc cacgtgaagg ccgaagggga cccccccgcc
    17041 cagttcgctg gagtactgaa gggccagctg gggcacgttc tcgaaggtgc aaccgcagta
    17101 cgtgctttcc gacttggcca gttccagcaa cacgaaagcg ttgaggtccc tggccgccat
    17161 caggacgccc tttatcgtca gggggttgcg cacgttcgcg gcgaggacga tggaacctgt
    17221 cctcctggcc gccgccgcga tgtccctccc gctcaccagg gccacgggtt caccgccgaa
    17281 aacggcctgg acgttgaggg gacgcttctc cagcagttca cggaaagacc tgctgctttg
    17341 atccatttca gaacacctcc cggtaatatc cgcatacatt gtacaatgaa gagggcccta
    17401 tgttcaccaa ggcccgccgg gcctttcgtc gatcttgggc ctttgacatt gacggtttcg
    17461 ctcgacccgt gataatataa ccggcgcgct acgggaacga tccttggggg atcgtctaat
    17521 cggcaggacg cttgactctg gatcaagaag tcggggttcg agtccctgtc ccccagccaa
    17581 gatccatgga aggccccatc gtctagtggt ctaggaccca gggttctcag tcctgtaaca
    17641 cgggttcgaa tcccgttggg gctgccagaa ataccgaggc ggacctcctt tttgaggggt
    17701 ccgccttttc taatcgggtg ccttcaggtt atacaccggc cggagcaatg ccgatacttc
    17761 cacggcctca ccgacttcat ccctgatgtc ctcccatctt ttgtatgcca tgggcgactc
    17821 atcgatggtc tccttgagga ccgacgtcga aaaaacaccc tccatgctct ttctgaagga
    17881 ccccatcgtg atggaacctt ccttcagcct gcgcttcatt tccttgcggc tgaagaggcg
    17941 acccgcaccg tggggagccg agtgattgta gtccgggtta cccttcccca cgccgaggac
    18001 tatgcctgaa gccatgttca gggggatgac cagcctttcg cccttgtggg ccgagatggc
    18061 acccttcctc acgatgccgt ccttttcgct gatgtagttg tggacgctct ccacaagggt
    18121 gccctcgttc aacctcaggc cgaagaagga gaggatcctg tcggcgatga tgaaacggtt
    18181 caggccggcg tactcctggg ctatccgcag ccacttcatg tactcgctcc cacccttatc
    18241 gagggggaga tgctccaggc cacgaggcag gccactggat gccccggcct tggactggta
    18301 gcgccgggcc aaaccttgga aaaaaccggc cacctggaag ccgaaatggc ggctgccggt
    18361 atgcaccgcg aggaatagcg ccccatcaag gccttcattg atctcgataa agtgattccc
    18421 gcccccgagg gtccccagct ggctccaagc ggggcctttc ggccgccgtg cgaggagttt
    18481 cccatagaaa ccttcatcgg cgagctctcc caactccctt gccctggctc cccggccggg
    18541 gaaacgggcg tcgaaccagt cgagcatcgc cgggccccgg tggcgggtct tccccagggg
    18601 gatgctgttc ctgacgaacc cgtcgagggc ggtgaagtct atgtcccctt tcttcactcc
    18661 ctcgatgggg gcgaggagaa caccgcagcc gatatcaacg cccaccacgt tgggtatgac
    18721 ggcgcccttg aacttcccgg tgaagccgat gacgcagccg gcccccaggt ggcagtcggg
    18781 catgatggcc accaggccgt cgacggcagg atgggccgct atgtccctta tctgggccat
    18841 ggtcggctcg tccacgtccc tggcatggat caccagttct atcatgacaa ccactccaat
    18901 atgggttttc aaggtcgtaa aaaattgccc cggttacatg ataacatctg taggccagca
    18961 aaaaggacgg tggaagacta catggaccca gaggaactgt tgacggcgcc ttcggctacc
    19021 cgggcgccct gcttcgcttt caggggcgac cttctgcttt tgaggacgga accgggggtt
    19081 gaactgccgg tttgggagga tattgcggga cttcccctct cggggcgaag cccgggtttc
    19141 tggataacgg gacggcccgg gaactgctgg gcatccctgg tggaggatgc acccctaccc
    19201 gaaggcatga gcctcgtgac cctgagggat gtctggtccc tcatgggcga acgcgctttc
    19261 ttcgaggccg gcagggcctt ccagatcatg gaatggtccc agcgtaaccg cttctgcgga
    19321 cgctgtgcga cccccatggt cgaggccgag aacgagacgg ccctggtgtg tccagcctgc
    19381 ggcttggtca gttaccccac ggtcaccccc gccgtcatcg tcgctgtcga aagggaggga
    19441 aggctgctcc tggccaggaa cactcgtttc cccaagggca ggtacagcgt catcgccggt
    19501 ttcgtcgaac ccggtgagac cctcgagggc accgtggaga gggaggtcat ggaagaggtc
    19561 tccgtcaggg tgaagaacat cacctacttt gggagccagc catggccctt cccccactcg
    19621 atgatggtcg gcttccgcgc tgagtgggag tcgggggaga tcatggccga cggcaacgaa
    19681 atagagagcg ctggctggta ctcccccgaa ggctttcctg atataccgcc gggctttagc
    19741 atttcaagga ggcttatcga cgacttcctg gccaggcatg cttgaggaag tgcccttcgc
    19801 gggaagggtc agttcttggg tttccccttg cggagttctt cctcccaggt gatcagggaa
    19861 tggtagtaga tgcggtcgcc gtcatcgtcc agccccctgg tgagttcgct gatatgggta
    19921 tgccccgcct ggaccctctc caggacgtgg ctgacggcct gttccggtat gctctccgcc
    19981 ccgcaggtat aaatatccag ggcgaggtac cgcgcttccg gccatgtgtg caccgacagg
    20041 tgggattcgc tgatcaccac gaccccgctg accccattgg gcgggaaccg gtggaaggaa
    20101 accgcccaaa cgtgggtgtt ggccttccgt gcggcctcga ccaggatctc ctgcatcctg
    20161 tcgacttcgc cgatgacctc actgcaacct gaagcctcga caatatagtg gatcccccta
    20221 gggtccatgg cttctcctcc ccgggatgct ctccctcgaa taagttcgaa aaacctagaa
    20281 ataataccct tttttcagtt ccgggtacag ggggaaggac ccggcaagat cccttacctt
    20341 cacggcaacc cggtccatcg ccgatcggtc ttgactttcc gagagcgcga cgtcgattat
    20401 cccggccacg agggccatct ccttctcctt catgcccctg gtggtgagcg ccggagtacc
    20461 gagcctgata ccgctggtca ccatgggctt ctcgggatca aagggtatca tatttttgtt
    20521 caccgtgata cccacccggc ccagtatatc cgtggcctcc ttaccggtga gccccttgct
    20581 gcggaggtca aggagcagca tgtggttatc cgtaccgccc gcgaccagcc tgtaccccct
    20641 ggcacccagt tcgcccgcca gggccttggc gtttttgacg atctgcgcgg cgtattcctt
    20701 gaactcgggc ctcatggcca tcaggaaaca ggtggccttt cccgcgatga cgtgcatgaa
    20761 cggcccgccc tgcataccgg ggaagacgtt gcggtccagg ccgggggcgt acttgccccg
    20821 acaaaggatg agggcgcctc gcggccccct caaggtctta tgggtcgtcg tggtgacaaa
    20881 atccgagtga gggacggggc tggggtggac accgccggcg accagccctg caatgtgggc
    20941 catgtcggcc atgagatagg ctcccacttc cttagctata tcgccgaagc gtttgaaatc
    21001 gatgaggcgg gggtaagcgc tcgcaccggc gacgatcatc ttgggcctgt gctcgcgggc
    21061 gagcctggcc acctcgtcgt aatcgatggt ctccctttcc ctgtcgacgc catagggtat
    21121 tatcttgaat aatcttccgg aaaagttcag cgggtgaccg tgggaaaggt gacccccttg
    21181 cgccaggtcc atggcgagga tggagtcgcc gggctcaagg acggtgaaat agacgcccat
    21241 gttggcctgg gtgcccgagt g
//
LOCUS       DAOSRG010000005        17954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_3259_length_17954_cov_10.252696, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000005 DAOSRG010000000
VERSION     DAOSRG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17954
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_17954_cov_10.252696"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLM30_00455"
     CDS             complement(<1..418)
                     /locus_tag="PLM30_00455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR53669.1"
                     /translation="MTLKRTADVVVIGAGIQGCSIAYHLAKSGIKDIVVLERSTICSG
                     STGRCGAGIRAQWGTEMNCRFGLAAIEKFEQLHEELGMDCGLHQGGYLVVAFEESEYE
                     HLKESIRLQNSLGIHTRAVSYDEAREICPGLSAEDAV"
     gene            complement(418..699)
                     /locus_tag="PLM30_00460"
     CDS             complement(418..699)
                     /locus_tag="PLM30_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOR53670.1"
                     /translation="MNEREEEIVICRCEEVTLGEIREWIDRGYQTFDELKRVLRVGMG
                     PCQGRGCREMILRELSLKTGKPVSEIHPGTFRPPSKPVKLSLLAAGGDT"
     gene            complement(696..1211)
                     /locus_tag="PLM30_00465"
     CDS             complement(696..1211)
                     /locus_tag="PLM30_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR53671.1"
                     /translation="MDEKEKLFNSGVLVAPRKGAVRPPEDLWRRKKNGLVIIECPQRI
                     PCNPCHTSCPTGAVKPFKDINDQPEIDYEKCTGCANCVAVCPGLACFVVDLTWGDEDQ
                     ALMKIPYEMLPIPSEGEIVECLDREGKAITRGKVIKVLEPLRDRTRVVHVEVPKSLVM
                     EVRAIKVVKQS"
     gene            complement(1215..2315)
                     /locus_tag="PLM30_00470"
     CDS             complement(1215..2315)
                     /locus_tag="PLM30_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR53672.1"
                     /translation="MRQTEVLVIGGGPAGLSAALEAASLGCQVTLVDGDLALGGQLIK
                     QTHKFFGSKVERAGTRGFKISEILLKELENHGDRVKVHINCTATGIYKEDGVVSCVIG
                     EEEYFRIKPERIVMATGAQERLIPFPNNHLPGVYGAGAVQTLMNVYGVIPGKRVLMVG
                     AGNIGLIVSYQLIQAGVEIAGIVEAMDKVGGYWVHAAKVRRLGVPIFLRHTIKEALGD
                     RVVTGALIQEIDDRFQPVGREKKIDADIICMAVGLTPTCELLWQAGCEMIYVPQLCGH
                     VALRDQFMRTSLPHVWVAGDAAGIEEATAAMVEGRIAGLDAARSLGKGDKEDLTERLE
                     DLQKRLGDLRGGEVGQKICQGLACCSIQGWGA"
     gene            complement(2331..2651)
                     /locus_tag="PLM30_00475"
     CDS             complement(2331..2651)
                     /locus_tag="PLM30_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOR53673.1"
                     /translation="MDLIREHPILDFKHGRKVRFTFDGEELEGFEGEPIAAALHANGI
                     RIYRLTPKRRQPRGFFCAIGKCSSCFLVVDGVPNVRSCITPLRTGMRIETQRDKGVIA
                     LDAE"
     gene            complement(2802..3479)
                     /gene="tsaB"
                     /locus_tag="PLM30_00480"
     CDS             complement(2802..3479)
                     /gene="tsaB"
                     /locus_tag="PLM30_00480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOR53674.1"
                     /translation="MKVLGIDCSAGWTGVGLFSEDAVIADVNFFAGRKQSHLLPILVE
                     DVLKKTGLGLSSLDAVAVTVGPGSFTGIKVGLSYACALAEGAGKKVVPACSLLALAAP
                     FLDGMSTLVPLIRARKGQVYFSVIEGEAKCPLVISPPGISPASRLTAMEGLNSTRFIL
                     AVGDPRLHQDLAGTGILPSLTGFFRGGTVALLGASSLDKAVDPGEVRALYIREPDTGP
                     GRDEGIK"
     gene            complement(3476..4012)
                     /gene="tsaE"
                     /locus_tag="PLM30_00485"
     CDS             complement(3476..4012)
                     /gene="tsaE"
                     /locus_tag="PLM30_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR53675.1"
                     /translation="MGGPSRIEKGGFNFVSSSPEETKTLAGHLSRYSFPGLVVLISGI
                     LGAGKTEFVRGFVSSMTRDEVRSPSFTLINEYKGDIPIAHVDLYRIERNASMEISLEE
                     YIREGFIVLLEWPENWDGEEPQETWKIELAMPEDFLQSAGRKNTRLFKATCEGEVACQ
                     RLERFISGFRSAWGEVER"
     gene            complement(4018..5565)
                     /locus_tag="PLM30_00490"
     CDS             complement(4018..5565)
                     /locus_tag="PLM30_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR53676.1"
                     /translation="MFLYNAEDIRQADRKAVELLGMPPAILMENAGKGVADAVKRYFP
                     SLSRVLIACGGGNNGGDGLVAARHLLREGYVVTVLLAAKEDNMTAETKTNLGILQNIG
                     CLLLHSETKTDGELTSLAAENDLVIDGLLGTGSKGAPRGEIGRVIRSLDKAVPIVAID
                     LPSGVDPSTGEIEGEVLAAKLTVAILARKIGHEVMPGRAFRGDLVTTDLGVEAGLLLP
                     EEGRAELLEPSMVSGLFPPLSPFLHKGDRGAVLVIGGSERYRGAPVLSALGALMGGSG
                     VVIAAVPADSLPLLSPHPEIIPLEVQKEDGVITPKAWDRILDRWGGKFSAVVLGPGLD
                     RGVISRELVRRVWVEWKGSLCLDGDALHALSCCGDLEPRPSGTVITPHEGEAGTLLST
                     DSAQVGQRRLKSVQDLSSRFGVTLLKGPCSLVSDGRRLRVNPHIVPGLSVPGSGDVLS
                     GLVGAFLAMGIQAFDAAAAAAFVHGSAGEFLQNQKGPHGITASEVALALPSARNRLLA
                     TPGKEGP"
     gene            complement(5572..5937)
                     /gene="acpS"
                     /locus_tag="PLM30_00495"
     CDS             complement(5572..5937)
                     /gene="acpS"
                     /locus_tag="PLM30_00495"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOR53677.1"
                     /translation="MIKGIGIDMCRISRLEKALKDPVFLEKVFTAKEKEYALSRSGRA
                     RHFASAFAAKEAFAKAGGWGLGQVGLKNVSLERRQGAPSLRLQGNAESLMQSLEARSA
                     HVSVTHEGDFAVAVVVLEG"
     gene            complement(6177..6251)
                     /locus_tag="PLM30_00500"
     tRNA            complement(6177..6251)
                     /locus_tag="PLM30_00500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6217..6219),aa:Val,seq:gac)
     gene            complement(6266..7120)
                     /gene="rlmB"
                     /locus_tag="PLM30_00505"
     CDS             complement(6266..7120)
                     /gene="rlmB"
                     /locus_tag="PLM30_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR53678.1"
                     /translation="MTTNPADSGKTGEDLVFGKNAVKTALETLPERCSMILLSDKIPA
                     SERDDLTAACKKSGVRWQSVKAAVVERLCPGVRHQGVVAKVLPVVLTDLDDFFEGLAA
                     AGGPALIIFADHVQDPQNLGALARSSEVFGAAGIVIPKRRSVLPTSTVMRTSSGAIAR
                     IPVVGIVNVARALREFKEKGFWIMGLDHCAEDILWECEIPEKAVLVIGSEGRGISRLT
                     RENCDFLVRIPMRGRTGSLNAATAGAIGMYEWFRTFSKGWSGTQGNTGPGGTRTGCGP
                     VDKPRGRG"
     gene            complement(7117..9942)
                     /gene="uvrA"
                     /locus_tag="PLM30_00510"
     CDS             complement(7117..9942)
                     /gene="uvrA"
                     /locus_tag="PLM30_00510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR53679.1"
                     /translation="MPQWIEVRGARQHNLKNIDVDIPKNRLVVITGPSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGVQDKPDVDDISGLSPAISIEQKGVSRNPRSTVGTV
                     TEVYDYLRLLFGRTGIPVCPGCGKAVERHTVDEILEMLLQKHEGEKVDILSPVVRGKK
                     GEFRNLFLQYQKEGFLRVRVDGTLFWLEEDIPLDKNRKHDIEVFIDRVTVESSRRDRL
                     AEAVENSLSLSEGYLMLLFGDQEVVITEKYACPDCDIILPEIEPRLFSFNNPYGACPA
                     CNGLGSHQYFSEELAVDPSAGVFEGAILPWKNKHYMLRKLAALARDRGWSLEGPYRDL
                     PEEVRKAILFGNETRVPMIYREGKKERSYMGRYEGLIPWLETRWKESDSESVLEELAG
                     YRAEDICQSCRGRRLRPEALSVRVDGLTIGEMVEMPISELLVRIHEPGFQGLMKEPAR
                     RIIEEISKRLSFMADVGVGYLSLSRRADTLSSGESQRIRLATQIGSKLSGVLYVLDEP
                     TIGLHPRDTSRLLGTLEAIRDLENTVLVVEHDRDTMVRADYIVELGPAAGEHGGRVTA
                     FGKPSEMVLADSLSGPLISGIDSGAALRPGPLPEPSAWITVAGAEEHNLKNIDVKIPL
                     GRLVCISGVSGSGKSTLLIEVLFKGLKRKLDRNYRYRAGKHRDIIGWEAFRNVVMIDQ
                     TPIGRTPRSNPATYTQLFTPIRELFASLPESRLRGYKAGRFSFNVRGGRCEACKGDGE
                     VRISMLFMPDVYVKCEVCHGKRYNRETLDIRYKGKNIAEVLDMTVDEARTFFGDIPRI
                     SRRLSVLQEAGLGYIRLGQPAPTLSGGEAQRVKLAEELGKQFRGHTLYILDEPTTGLH
                     YLDVRKLIVLLQKIVDQGNTVLLIEHNLDVLASADHIIDLGPEGGLQGGTVVTTGSPD
                     EITRKGKGHTARHLGEFLSNLKRRGPQ"
     gene            complement(9872..11911)
                     /gene="uvrB"
                     /locus_tag="PLM30_00515"
     CDS             complement(9872..11911)
                     /gene="uvrB"
                     /locus_tag="PLM30_00515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR53680.1"
                     /translation="MNDNLKAFDLHSDWPPAGDQPEAIDRLVEGFRKGERFQSLLGVT
                     GSGKTFAMANVIERLQRPALVLAHNKTLAAQLYSEFKEFFPGNAVHYFVSYYDYYQPE
                     AYVPVSDVYIEKDASINERIEKLRLATTKSLLERRDVIVVASVSCIYGLGKRKAYEEA
                     VFRFALGDSFERRDFLARLVTNHYDRNDVDLHPGTFRARGGSIEVFPAYSDTALRVYL
                     EDDVIVRIDEIEPLTGKNILSKPNAAVFPAQHYITTPDAIADALPRIEEELEERLEYF
                     RSRGKLLEAQRLEMRTKYDMEMLSEVGYCSGIENYSRILDGREPGETPGTLLDFFPED
                     RLFFIDESHITIPQVQGMYNGDRARKQTLVDHGFRLPSCLDNRPLKWEEFCSFLKQAC
                     FVSATPGDWELSVSSTVAELIVRPTGVVDPEVIVIPATGQIDDLTERLRKVAARGERA
                     LVTTLTKRSSEDLADHLSEIGFGVKYIHSELDAFERTELIRDLRKGEISVLVGVNLLR
                     EGLDLPEVSFVGILDADREGFLRSRRSLIQMIGRAARNTAGKVILYADQETGSIKTAL
                     DETERRREKQLSFNRSHNITPRSVQKKVMSLLPEEQAEDVPQEISVRSGGEGFTSQEL
                     ERMMWKAVEDLDFERAAVLRDILQKNQGGGFRRAAMDRGKRGKAAQPEKHRRRHT"
     gene            complement(12031..13917)
                     /gene="ftsH"
                     /locus_tag="PLM30_00520"
     CDS             complement(12031..13917)
                     /gene="ftsH"
                     /locus_tag="PLM30_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOR53681.1"
                     /translation="MGKMAKNLGLYLVMIVLVVSLVNMFLSPAQNPEESELISYSTFL
                     NYFHEGKLERVSIEGDLIKGTREGGVRFRTIAVGVGDLAPQLAEKGVQVEVLPPAKSP
                     WWVTMMSSLFPTLLLIGAWVFILYHMQGGSNKVMSFAKSKAKLFLDNRPKVTFADVAG
                     CDESKEELKEVVDYLRDPARFSRLGAKVPKGVLLLGPPGAGKTLLARACSGEADVPFF
                     SISGSDFVEMFVGVGAARVRDMFEQARKNQPCIVFIDEIDAVGRQRGAGLGGGHDERE
                     QTLNQLLVEMDGFNVSSGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDRNGREAI
                     LKVHLRDMKTDDDVNLEVLAKRTPGFVGADLANLVNEAALLAARRDRKSISMAEFEEA
                     IDRVLAGPERRTRLISDHEKEIIAFHEVGHALVAKMVPGCDPVHKISIIPRGHKALGY
                     TLQLPEEDRFLMSKKELINRICVLLGGRVAEEIHFSDITTGAQNDLERATQIARQMVT
                     EFGMSDSLGPVTLGRKQHEVFLGRDIMEDRNYSDEVAYAIDQEVRRIVEGCYERVRAI
                     LKENHEAHVRIARTLLAEEVIEGDRLDALLEEGITMPAEGEDEDEGCSAEPSRDREEG
                     GSVA"
     gene            complement(13969..14505)
                     /gene="hpt"
                     /locus_tag="PLM30_00525"
     CDS             complement(13969..14505)
                     /gene="hpt"
                     /locus_tag="PLM30_00525"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOR53682.1"
                     /translation="MECDLGEVLIRRKTIQDRVRELAFDIGSAYGDRDLVLIGVLKGA
                     VVFLSDLIRELPVSMATELDFLAVASYGTSTESSGVVRIIKDLDVDISGRNVMIVEDI
                     VDTGLTLSYLVSILREREPAVLEVCSLLDKSARRKVNVDITFRGFEIPDEFVVGYGLD
                     FSGKWRNLPEIYTLKRKG"
     gene            complement(14495..15904)
                     /gene="tilS"
                     /locus_tag="PLM30_00530"
     CDS             complement(14495..15904)
                     /gene="tilS"
                     /locus_tag="PLM30_00530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOR53683.1"
                     /translation="MKDHPFVERWSPGTRSLFRRFEAAGRDQGWWDHDGPIVAAVSGG
                     SDSMALFWLLRFRWKGRVIAAHLEHGFRQETSLRDAEFVERICREWNTECHVERLDIP
                     SMKRRGETLEEAGRRERYAFLRRVASCSAAPLIATGHTSDDLVETVFLNLLRGTGIRG
                     LRGIPWARERVVRPIMGISRDELRGLLLEASVPWMEDETNLETKYFRNKIRHVFFPLL
                     CNEGNIRFKDHLLDLSAEAGELEAAREKESRSMAAWCRRWQPFALRSWDLDLLRRMND
                     QAIQSLIAFEGRDLVLSILSRRKTSVLLGLIRKKEGRWRFQWEGDLEICASGRTASLV
                     DRKVFSAKEPDRVTFAPHGEYGSIRWGQWRFDWRYATDLPPALGVGSCRIPVEGAGPV
                     EILAAGAADKRDLHEGAIPWWAGRIWPLVRSSGTSWIPFTVPPGSTVMRSNGKSGPSL
                     QLQARIIDKMRKGETEDGV"
     gene            complement(15956..17644)
                     /locus_tag="PLM30_00535"
     CDS             complement(15956..17644)
                     /locus_tag="PLM30_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOR53684.1"
                     /translation="MTEDQDERGGSFIEQIIREDLESGRVSKVITRFPPEPNGYLHIG
                     HAKSICLNFGIAEQFDGECNLRFDDTNPSKEETEYVESIMKDVSWLGFHWAKLRFASD
                     YFDQFHEWALELVQAGKAYVDHQTADQIRQNRGTLTEPGVESPYRDRPVEENMELFRK
                     MKEGYFPEGECVLRAKIDMSHPNLNMRDPVMYRILHQSHHRTGDKWCIYPMYDFAHGY
                     EDAIEGVTHSICTLEFEDHRPLYDWFIENVSVPSRPRQYEFARLNLTYTVMSKRKLLE
                     LVSNRFVSGWDDPRMPTISGLRRRGYTPSSIRTFCKKIGVSKANSLVDVELLHHCIRD
                     ELNAVAPRAMAVLRPLRLIIENYPEGLSEEIEVLVNPEAETPSYRKVPFGRELLIERE
                     DFMENPPAKFFRLSPGSEVRLKHAYIIKCNGFDKGPDGRVIAVRCEYYPESRGGEAPD
                     GRRIKGTLHWVSAIHAVKAEVRLYDHLFTLESMDEMEEGTDYEDYLNPASLEVIKDCM
                     VEPLLTEAAPMDRFQFFRLGYFVADQEHRKDHPVFNRTIPLKDSWAKAMSRVLK"
     gene            complement(17857..17933)
                     /locus_tag="PLM30_00540"
     tRNA            complement(17857..17933)
                     /locus_tag="PLM30_00540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17897..17899),aa:Met,seq:cat)
BASE COUNT         3657 a         5574 c         4679 g         4044 t
ORIGIN      
        1 ccacggcatc ttcagcggac aaccccggac atatctccct ggcttcatcg taggagacgg
       61 ccctggtgtg gatcccgagg ctgttctgaa gccggatgct ctccttgagg tgctcgtatt
      121 cactttcctc aaaagccacc accaggtacc ctccctggtg gagcccgcaa tccatgccca
      181 gttcttcatg tagctgttcg aacttctcta tcgcggcaag cccgaacctg caattcatct
      241 cggtacccca ctgggccctt ataccggcgc cgcatcttcc cgtcgatccg gaacaaatgg
      301 tactcctctc gaggactacg atatccttga tcccggactt cgccaggtgg taggcaatgg
      361 aacagccctg gatcccggcc ccgataacaa cgacatcggc agtcctcttc aaggtcatca
      421 cgtatccccg ccagcggcta gaagcgacag tttgaccggt ttggaggggg gcctgaaagt
      481 accggggtgg atctcggaga caggttttcc cgttttcagc gataactccc tcaggatcat
      541 ctcgcgacac cctctcccct ggcatggtcc catgcccacc ctgaggaccc ttttcagctc
      601 gtcgaaggtt tgatagcccc ggtcgatcca ttccctgatc tctcccaggg tgacctcctc
      661 gcaacggcag atgacgatct cttcttccct ttcattcacg actgtttcac caccttgatc
      721 gcgcggacct ccatgaccag ggacttcggg acctccacat ggacgactct tgtcctgtcg
      781 cgcaggggtt cgagaacttt gatgacctta ccgcgggtta tggccttgcc ttccctgtca
      841 aggcactcca cgatctctcc ctccgagggg atggggagca tttcataggg gatcttcatt
      901 aacgcctggt cttcatctcc ccaggtgagg tccacgacaa agcaggccag gccggggcag
      961 acggccacgc aattcgcgca gcctgtgcat ttttcataat cgatctcagg ctgatcgttg
     1021 atatctttga aaggtttcac cgctcccgta gggcagcttg tgtggcaggg gttgcaaggt
     1081 attctctggg gacattcgat gatgacgagc ccgttcttct ttcttcgcca gagatcctcc
     1141 gggggtcgca ccgctccttt ccgcggggcg acgagcaccc cgctgttgaa caatttctcc
     1201 ttttcgtcca ttccttacgc cccccatcct tggatgctgc aacaggcaag gccctggcag
     1261 atcttctggc cgacctcccc gccccgaagg tcaccaaggc gcttctgcaa gtcttcgagt
     1321 cgttcagtca ggtcctcctt gtcgcccttc cccagtgacc tggcggcgtc taggcccgct
     1381 atcctcccct ccaccatggc agcggtagcc tcctcgatac cggccgcatc gcccgccacc
     1441 cagacgtggg gaaggcttgt cctcatgaat tgatccctca gggcgacgtg cccgcatagt
     1501 tggggtacat agatcatttc gcacccggcc tgccaaagga gttcgcaggt aggtgtcaac
     1561 ccgacggcca tgcagatgat gtcagcgtcg atctttttct ctctgccaac cggttggaac
     1621 cgatcatcta tctcctgtat cagggctcct gtcacgaccc ggtctcccag ggcctccttt
     1681 atcgtgtgtc ttaggaaaat gggaaccccc agcctcctga ccttggccgc atggacccaa
     1741 tatcctccaa ccttatccat ggcttcaacg atgcccgcga tctccacacc agcctggata
     1801 agctgataac tgacaataag gccgatatta ccggcgccca ccatgagtac ccttttcccc
     1861 gggatcacgc cgtagacatt catcagcgtc tggaccgccc cagcaccgta gacgccgggg
     1921 aggtgattgt tcgggaaggg gatgaggcgc tcctgcgccc cggtggccat gacgatcctt
     1981 tccggtttga tgcggaagta ttcttcttcc ccaataacgc aggagacaac cccatcttcc
     2041 ttgtagatac cagtcgcagt acagttgata tggaccttga ccctgtctcc gtggttttcc
     2101 agttccttga ggagtatctc cgagatcttg aaccctctcg tcccggccct ctcaaccttt
     2161 gatccgaaga atttgtgggt ttgcttgatc agttggccgc ccagagccag gtcgccatcc
     2221 acgagggtga cctggcaacc cagcgaggcc gcctcaaggg cagcgctgag accggcggga
     2281 cctcctccta ttacaagtac ctcggtctgc ctcactctta tcacctgccc ctattctgca
     2341 tccaaagcga tgacgccctt gtcgcgctgt gtctcgatcc gcattccggt cctcagcgga
     2401 gtgatgcagg accgcacatt gggcacgcca tcaacgacca ggaagcagga tgagcatttc
     2461 ccgatggcac agaagaaccc cctgggctgc cgcctctttg gggtcaacct gtagatcctg
     2521 atcccgttcg cgtgaagggc cgccgcgatc ggttctccct caaaaccctc caactcctca
     2581 ccatcgaagg tgaacctgac tttcctcccg tgtttgaagt cgagtatcgg gtgttcccgg
     2641 atcagatcca tgaccaccaa tccctcccct tgctcgatgt tgaaacgcta agggctgggg
     2701 aatcttctcc cgcgccgctt ccaccgcctt tttccactta aattaaagaa atcgatggca
     2761 aatatgttac ccttcagtgc caggattgac cagagacttt ttcatttgat cccctcgtcc
     2821 cgacccggcc ccgtatcggg ttccctgatg taaagagccc tcacttcccc tgggtcgacg
     2881 gccttatcca gggaagaggc ccccaacagg gcaacagtcc cgcccctgaa aaagcctgtc
     2941 aatgagggaa ggatccctgt tcccgccagg tcctggtgca aacgagggtc cccaacggcc
     3001 aagatgaaac gggtggagtt caggccctcc atcgccgtca ggcgactcgc gggcgagatg
     3061 ccgggcggcg aaatgacgag ggggcacttt gcttcgccct cgatcacaga aaagtaaacc
     3121 tggcccttcc tggctcttat cagggggacc agcgtactca ttccgtccag gaagggcgcg
     3181 gccagggcca agagggaaca ggccggcact acctttttcc ccgcgccttc agcaagggcg
     3241 caggcgtagg aaagccccac cttgatccct gtaaaggatc ccggccccac ggtcaccgcg
     3301 acggcatcca gggatgaaag acccagacct gtcttcttca ggacatcttc aacaaggata
     3361 ggaaggagat gagactgctt tcttcctgca aaaaagttga catcggcgat aacggcatct
     3421 tccgagaaaa ggccgacccc ggtccagcct gcagaacaat ctatacccag cactttcatc
     3481 tttcgacttc cccccatgcc gatcgaaacc ccgagatgaa ccgctccagt ctttgacagg
     3541 cgacctcgcc ttcgcaggtg gctttaaaga ggcgggtgtt tttccttccg gcactctgta
     3601 aaaagtcttc cggcatggcc agctcgatct tccaggtctc ttggggctct tcaccatccc
     3661 agttttcggg ccattcaaga aggacaataa aaccttcacg gatgtattct tcgagggaga
     3721 tctccatcga cgcgtttctt tctatcctgt atagatctac gtgggcgatc gggatatcgc
     3781 ccttgtactc gttgatcagt gtgaaggatg gactcctgac ctcgtcccgg gtcatcgacg
     3841 agacaaaacc ccttacgaat tcagttttac cggcccccaa gatgccggat ataaggacaa
     3901 ccaagccagg gaaagagtac cgcgagagat gtcccgccag ggttttcgtc tcctcggggc
     3961 tcgatgatac gaaattgaac ccgccttttt cgatccggct tgggccaccc acggaggtca
     4021 cggcccttcc tttccggggg tcgccagtaa acggttccgg gcggaaggga gtgcaagggc
     4081 gacctcagag gctgtgatcc cgtggggtcc tttttggttc tggagaaatt cacccgccga
     4141 accgtggaca aaggcggccg cggccgctgc atcgaaagcc tggataccca tggccaggaa
     4201 agccccaaca agaccggaaa ggacgtcccc agaccccggt accgaaaggc ccgggacaat
     4261 atgaggattg actcttaacc tccgaccgtc ggataccaac gaacatggcc ccttgaggag
     4321 ggtcacgccg aatcgggatg aaagatcctg cacagacttg aggcggcgtt gacccacttg
     4381 ggcactgtct gtggataaca aggtgcctgc ctcaccctcg tggggtgtaa taacggtccc
     4441 gctgggtcta ggttcaaggt ctccacagca ggaaagggca tgaagggcgt ccccatccag
     4501 gcaaagggag cctttccatt caacccatac cctccggacc aactcccggg agatgacccc
     4561 cctgtcaagg cccggcccga gcactacggc cgaaaatttt cccccccacc gatccaggat
     4621 cctgtcccag gccttagggg taataactcc gtcttccttt tggacttcca gggggatgat
     4681 ctccgggtga ggggataaca gggggagaga atcggccggt accgcggcta tgacgacgcc
     4741 ggatcccccc atgagcgccc ccagggctga caataccggt gctccccggt acctctcgct
     4801 ccccccgata acgaggacgg cgcccctgtc acccttgtga aggaaaggcg acagcggggg
     4861 gaagagtccc gaaaccatcg atggttccaa cagttccgcc cttccctctt caggcagcag
     4921 gaggccggcc tccactccca ggtccgttgt taccaggtcg ccccgaaagg cccttcccgg
     4981 catcacctcg tgaccgatct tgcgggctag gatcgccacc gtaagctttg cggccaggac
     5041 ctcgccctcg atctcccccg ttgaggggtc gaccccgctt gggagatcga tcgcgacgat
     5101 ggggacggct ttatccagtg agcggatgac cctccctatc tcccccctgg gggcgccctt
     5161 ggaccccgtt ccaagcaggc cgtcaatgac caggtcattc tcggccgcca gcgatgtgag
     5221 ctctccgtca gttttggttt cggagtgaag aaggaggcag ccgatgtttt gaagtatccc
     5281 gaggtttgtc tttgtttcgg cggtcatatt gtcctccttg gccgctagga ggaccgtaac
     5341 aacatagcct tccctcaaaa gatgccgggc ggcaaccagg ccgtcaccgc cgttgtttcc
     5401 tccgccgcag gctatcaata ccctggaaag ggaggggaaa tatctcttga cggcatcggc
     5461 cacacccttg cccgcgttct ccatgagtat ggccgggggc attcccagga gttccacagc
     5521 cttccggtca gcctgtctga tatcctcggc attgtagagg aacaagatag ctcacccctc
     5581 aagtacaacg actgctacag caaaatcacc ttcatgggtc acgcttacgt gggcgcttcg
     5641 ggcttccagg gactgcatca gcgactcggc attgccctgg aggcgcaggg acggcgcgcc
     5701 ttgtcgacgt tctagggaga cgttcttcaa acctacctgt cccaaccccc agcccccggc
     5761 tttggcaaag gcttctttcg cggcgaaggc cgaggcaaag tgcctggctc tccccgacct
     5821 ggacagggcg tactccttct ctttcgcggt aaagaccttt tccaggaaca ccgggtcctt
     5881 gagagctttt tccagacggg agatacgaca catgtctata cctataccct ttatcatctt
     5941 gtccctcctc gcccgtcaac tgattatagg acaaaagctg cgaccctttc ttttctttcc
     6001 ataaatggca ggggcaaagc cagatcttcc aggacaggac cgggatcgct ccgtgttctc
     6061 ccttcctcca agggaaggta ttaaaaaacc cctcccgtca agaggaaggg ggcccgaaaa
     6121 tacattaccc cgagagagcc taaccaaggc cacgcggggt aaagtcacat catctatggt
     6181 gggtggtgca ggactcgaac ctgcgacctc ttccgcgtca aggaagcgtt cttcctctga
     6241 actaaccacc caagcgtgtt gcattctacc cgcggccccg aggcttgtca acaggaccgc
     6301 acccagtgcg ggtgcctcca gggccagtgt tcccttgcgt tcctgaccac ccttttgaaa
     6361 aggttctgaa ccattcgtac atgccgatcg cgcccgccgt tgccgcgttg agtgaccccg
     6421 tacgccctct catggggatc ctcacgagaa agtcgcagtt ttcacgggtc agtcttgaaa
     6481 taccacggcc ttcgcttcct attacgagga cagccttttc cgggatctcg cactcccaga
     6541 ggatatcttc cgcacaatga tccaggccca tgatccagaa ccctttttcc ttgaattccc
     6601 taagtgcccg ggcgacgttc acgatcccca cgacggggat cctggctatg gccccggaac
     6661 tggtcctcat gacggtcgaa gtggggagaa cggagcgcct tttgggtatg acgatccccg
     6721 cggcgccgaa gacctccgaa gacctggcga gagcgccaag gttctgggga tcctggacat
     6781 ggtcggcgaa aatgatcaaa gcagggccgc cggcagccgc caagccttca aaaaagtcat
     6841 ccaggtcggt caacaccacg ggtagcacct tggccacgac accctggtgg cggacgccgg
     6901 gacaaagacg ctctaccacg gctgccttta ccgactgcca ccggacaccg gactttttac
     6961 aggctgcggt gagatcatct ctctccgacg caggtatctt gtccgagaga aggatcatgg
     7021 agcatctctc cgggagagtc tctagggcgg ttttcacggc gttcttccca aagaccaggt
     7081 cctcgcctgt tttaccgctg tctgcaggat tggtcgtcat tgaggccctc tcctcttcaa
     7141 gttgctcagg aactctccca ggtggcgggc ggtatggccc ttcccttttc gggtgatctc
     7201 gtccggagat cccgtcgtta ctacggtccc gccctggagg cctccctccg gacccaggtc
     7261 gatgatgtga tccgccgagg cgagaacgtc caggttgtgt tcgatcagga ggaccgtatt
     7321 gccctggtcg acgatcttct ggaggaggac gatcagtttc ctgacgtcaa ggtaatgaag
     7381 ccccgtcgtc ggttcatcga ggatataaag ggtatgccca cggaactgct tgcccagttc
     7441 ttctgcaagc ttgaccctct gtgcctcccc tccgctgagc gttggggctg gttgcccgag
     7501 acggatatag cccagcccgg cttcctggag gacggaaagc cttctcgata tcctggggat
     7561 gtcgccgaaa aaggtccttg cctcatcgac ggtcatgtcc aggacttctg ctatattctt
     7621 ccccttgtat cttatatcca gcgtttccct gttataccgc ttcccatggc agacttcgca
     7681 cttgacatag acgtcgggca tgaaaagcat ggaaatgcgg acctcaccgt cccccttgca
     7741 agcctcgcaa cggccgcccc tgacgttgaa gctgaagcgc ccggctttat aacccctcag
     7801 cctggactcg ggaagggacg cgaacagttc cctgatcggg gtgaacaact gggtataggt
     7861 agccgggttt gaccgcgggg ttctccctat gggagtttgg tcgatcatta ccacgttcct
     7921 gaacgcttcc cacccgataa tatccctatg cttacccgcg cggtacctgt aattcctgtc
     7981 cagtttcctc ttcaagccct tgaaaagcac ctcgataaga agggtacttt tgcccgaccc
     8041 ggataccccg cttatgcata cgagccgccc cagggggatc tttacgtcta tatttttcaa
     8101 attatgctct tcggccccgg cgacggtgat ccaggccgac ggttccggta acggccccgg
     8161 acgtagcgcg gcgcctgaat cgatccccga aattaagggc cctgaaagag aatcggccaa
     8221 gaccatttcc gacggtttgc cgaaggccgt caccctcccc ccgtgttcgc ccgcggcagg
     8281 accgagttca acgatatagt cggccctgac catggtatcc cggtcatgct caacgaccag
     8341 gacggtgttt tccaggtccc ttatggcctc cagggtaccc aggagccttg acgtatccct
     8401 cgggtgcagc cctatggtcg gctcatccag gacgtagagg acgccgctga gtttcgaccc
     8461 tatctgggtg gctagcctga ttctctggct ctcccccgag ctgagtgtat cggccctcct
     8521 ggatagcgaa aggtagccta ctcccacgtc ggccatgaag gagagtcttt tcgagatctc
     8581 ttcgatgatc ctcctggcgg gttctttcat caggccctgg aaacccggtt cgtggatcct
     8641 caccagcagc tcggagatag gcatctccac catttcgcca atggtcagcc cgtcgacccg
     8701 gacagagaga gcctccggcc ttagtcttct acccctgcag gattgacata tatcctccgc
     8761 cctgtaaccg gccaattcct ccaggaccga ttccgagtcg gactctttcc atcttgtctc
     8821 cagccagggg atcaaccctt cgtaccttcc catgtaggac ctttcctttt tcccttcgcg
     8881 gtagatcatc gggactcggg tctcattccc gaacaggatg gcctttctca cttcttccgg
     8941 caggtcccgg taaggccctt ccagcgacca gccccgatcg cgggcgaggg ccgccagctt
     9001 cctaagcatg tagtgcttgt tcttccaggg gaggatcgct ccttcgaaaa ccccggcgga
     9061 aggatcgacg gccagttcct ctgagaagta ctggtggctg ccgaggccgt tacaagcggg
     9121 acaggccccg taagggttgt tgaaggagaa cagtcggggt tcgatctcag ggagaatgat
     9181 gtcgcagtca gggcaggcgt atttttcggt gatgactact tcctggtcgc cgaaaagcag
     9241 catcaggtaa ccctcgctga gggagaggga gttctcgaca gcctcggcaa gcctgtccct
     9301 cctgctgctc tccacggtga cccggtcgat gaacacctcg atatcatgct tccgattctt
     9361 atctaaagga atgtcctcct caagccagaa aagggtccca tcaactctga cccttaggaa
     9421 tccctccttc tggtactgga gaaaaaggtt acggaactcg cccttttttc ctctgaccac
     9481 gggggagagg atgtccacct tttccccctc gtgcttctgc aaaagcattt ccaggatctc
     9541 atccacggtg tgcctttcaa cggctttgcc gcacccggga caaacgggga tcccggtcct
     9601 accgaaaaga agccggaggt agtcgtaaac ttcagtcacg gtcccgacag tcgacctcgg
     9661 gttgcgggag actccctttt gctctatgga aatagccggc gagagcccgg aaatatcgtc
     9721 cacatcgggc ttgtcctgaa cacccaggaa ctgcctggcg tacgaggaga gtgattcaac
     9781 gtaacgccgt tgaccctcgg catagatcgt gtcgaaggcc agggaagact tccccgaccc
     9841 cgaaggccca gtgatgacga ccagcctgtt tttaggtatg tctacgtcga tgtttttcag
     9901 gttgtgctgc cttgcccctc ttacctctat ccattgcggc acgtctgaat ccgcctccct
     9961 ggttcttctg gagaatatcc ctcaggacag ccgccctctc aaaatcgaga tcctcgacgg
    10021 ctttccacat catcctctcc agttcctggc ttgtaaaacc ttcccccccc gacctcaccg
    10081 atatttcttg ggggacatct tcggcctgtt cctccgggag cagggacatg accttctttt
    10141 gcacgctacg gggggtgata ttatgggacc ggttgaaact caactgcttt tctctccttc
    10201 tctcggtttc atcgagggcg gtcttaatgc tacccgtttc ctggtcggcg tagaggatca
    10261 ctttgcccgc cgtgttcctt gcggcccggc cgatcatctg gataagggac ctccgggagc
    10321 gcaggaaccc ctccctgtcg gcgtcaagga tccccacgaa ggaaacctcg ggaagatcaa
    10381 gtccctccct gaggaggtta acgcccacca ggaccgatat ttctcccttg cggaggtccc
    10441 tgatcagttc cgtcctttcg aaagcgtcga gctcggaatg gatatattta accccgaaac
    10501 ctatttcaga aaggtgatcg gcaaggtcct ccgaggaacg tttcgtgagg gtcgtgacca
    10561 gcgccctctc ccccctggcg gcaaccttcc tgagcctttc ggtcaggtcg tcgatctgcc
    10621 ctgtcgcggg gatgacgatc acttcgggat cgacaacacc ggtcgggcgg acgatcaatt
    10681 cggccaccgt cgaggaaaca ctcaattccc agtctcccgg cgtggccgac acgaaacagg
    10741 cctgtttcaa aaaagaacag aactcctccc acttgagggg gcggttgtcc aggcacgagg
    10801 gcagcctgaa accgtgatcc accaaggtct gtttcctggc cctgtccccg ttgtacattc
    10861 cctggacctg ggggatcgtg atatgagatt catcaatgaa gaaaagacga tcctcgggga
    10921 agaaatcaag caaggtcccc ggtgtttcgc cgggttctct cccgtcgagg atgcgggagt
    10981 agttttcaat tcccgaacag taccctactt cggaaagcat ttccatatcg tacttcgtcc
    11041 tcatctccag gcgttgcgct tcgaggagtt ttcccctgga tctgaagtac tccagtcttt
    11101 cttccagttc ttcctcgatc cgggggaggg cgtcagcgat ggcatcgggg gtcgtgatgt
    11161 aatgctgggc cgggaaaacc gcggcattgg gtttcgagag gatgttttta cccgtcagtg
    11221 gttcgatctc atcgatcctt acgatgacat catcttccag gtaaaccctc aaggccgtgt
    11281 cactgtaggc cgggaaaacc tcaatggatc cgcccctcgc ccggaaagtg cctggatgga
    11341 ggtccacgtc gttcctgtcg taatggttgg taaccagcct cgcgaggaaa tccctgcgct
    11401 caaaactatc ccccagggca aatcggaaga cggcttcctc gtaggccttt ctttttccca
    11461 ggccgtagat acatgaaacg ctcgccacta cgatgacatc tctcctttca aggagcgatt
    11521 tcgtcgtggc aagtctgagt ttttcgatcc tctcattaat ggaggcgtcc ttttcaatat
    11581 agacatcgga gaccggtaca taggcctccg gttggtaata gtcgtagtaa ctgacgaagt
    11641 agtggaccgc gttacccggg aagaattctt tgaattcact gtatagctgt gccgccagcg
    11701 tcttgttgtg cgccaggaca agggcgggcc tctgcaatct ttctatcacg ttggccatcg
    11761 cgaaagtctt gccgctgccg gtgacaccta aaaggctctg gaaacgctcg ccttttctga
    11821 aaccttcgac aaggcgatcg atggcttccg gctgatcccc cgccggcggc caatcggagt
    11881 gtagatcaaa ggctttcaaa ttatcgttca aaacaacctc cctcggacat tctcaaagcg
    11941 aaaagagcgc tcgacctcaa aggatccggc cgaacgctcg aggctttcat cgggcgggaa
    12001 aggccgccct ttcggcttgg gcctcctctc tcaggcgacg gaccctcctt cttccctatc
    12061 cctcgacggt tcagcggaac aaccttcgtc ttcgtcttct ccttccgccg gcatcgtgat
    12121 tccctcttcg agcaaagcat cgagccggtc gccttcgata acctcttcag ccaggagggt
    12181 cctcgcgatc ctcacgtggg cctcgtgatt ctccttgagg atggcacgga ccctttcgta
    12241 acacccttcg acgatgcgcc gtacttcctg gtcgatagcg taggccacct catcgctgta
    12301 attgcggtct tccatgatat cccttcccag gaaaacctcg tgctgctttc ttcctagggt
    12361 gaccggcccc agggaatcac tcattccgaa ctccgtcacc atctgcctgg ctatctgggt
    12421 ggctctttcg aggtcgttct gggcaccagt ggtgatgtcc gaaaaatgga tctcctcggc
    12481 cacccttcct cccaagagca cgcatattct attgatgagc tccttcttgg acatgaggaa
    12541 ccgatcctct tccggcagtt gcagggtgta acccaacgcc ttgtggcccc gggggatgat
    12601 cgatattttg tggacggggt cacagccggg gaccatcttg gcgacgaggg catgtcccac
    12661 ttcgtgaaac gcaatgatct ccttttcgtg atcactgatc agcctggtcc tccgctcggg
    12721 tcccgccagc accctgtcga tggcttcctc gaattcagcc atggatatgc ttttcctgtc
    12781 cctcctcgcc gccaggaggg cggcctcgtt cacgaggttg gccaggtcgg ccccgacaaa
    12841 accgggggtc ctcttcgcca gaacttcgag gttcacgtcg tcatcggtct tcatatccct
    12901 aaggtggacc ttgaggatgg cctcccgccc attcctgtcc gggcggtcga ccacgatatg
    12961 acggtcgaac cgaccaggcc tgagaagagc cgggtccagg atgtcaggcc ggttggtcgc
    13021 agctatcagg ataatccccg atgacacgtt gaagccgtcc atttctacca agagctgatt
    13081 gagcgtctgc tccctctcat cgtgtccgcc gccaaggccg gccccacgct gcctccccac
    13141 ggcatcgatc tcatcgatga acacgatgca gggctgattc ttcctggcct gctcgaacat
    13201 gtcccgcacc ctggcagcgc ccacacccac gaacatttcg acgaaatcgg aaccgctgat
    13261 gctgaaaaag gggacatcgg cctcgcctga acaggccctg gccagaaggg ttttccctgc
    13321 gccggggggc cccaggagaa gcactccttt ggggaccttg gccccaagcc tggaaaacct
    13381 ggcggggtcc ctaaggtaat ccacgacctc cttcagttcc tcctttgatt catcacaccc
    13441 ggcgacgtcg gcgaaggtga ccttgggcct attgtccaga aaaagtttcg ccttgctctt
    13501 ggcaaaactc atcaccttgt tgctcccgcc ctgcatgtga tagaggatga acacccaggc
    13561 tccgatcagg agcaaggtgg ggaaaaggga ggacatcatc gtcacccacc agggggattt
    13621 cgccggggga aggacctcca cctggacgcc cttttccgcc agctggggcg ccaagtctcc
    13681 aaccccgacc gctatggtac gaaaccggac gcctccctca cgggtgcctt ttatgaggtc
    13741 gccctcgatg gagacccgtt ccagtttccc ctcatggaaa tagttcagaa aggtgctgta
    13801 actgatcagt tcactctcct cggggttttg agcaggtgaa aggaacatgt tcaccagact
    13861 tacaaccagc acgatcatca ccaggtaaag cccgaggttt tttgccatct tccccaatgc
    13921 gttcccacct ctcgttaaac agcacaaacc accctgcaga gaaagagttt aaccctttct
    13981 cttcagagtg tatatctcgg gaaggttcct ccacttcccc gaaaaatcca ggccatagcc
    14041 taccacaaac tcgtcgggta tttcgaagcc cctgaaagta atatcgacat tgacctttct
    14101 tcgagccgat ttgtcgagca gcgaacagac ctccagcacg gcgggctccc tctccctcag
    14161 gatgcttaca aggtaggata gagttagtcc cgtgtccaca atgtcctcga cgatcatgac
    14221 attcctgccg ctgatgtcga cgtccaggtc cttgattatc cgcaccaccc cgctggattc
    14281 ggttgaagtc ccgtaggatg ccacggcgag gaaatccagc tcggtcgcca ttgaaaccgg
    14341 tagttccctt atcaggtcgg aaaggaagac tacggccccc ttcaaaacac cgatgagcac
    14401 gaggtccctg tcgccgtaag cggaaccgat gtcgaaagcg agctccctga ccctgtcctg
    14461 gatggtcttt ctcctgatca agacctctcc gagatcacac tccatcttcg gtctctcctt
    14521 tcctcatctt atcaatgatc ctcgcctgaa gttgcaggga ggggccggac tttccattac
    14581 tcctcatcac tgttgaacct ggcgggacgg taaaggggat ccaggaagtc cccgaagatc
    14641 tgaccagggg ccaaatcctc cccgcccacc agggaatagc cccttcgtgt aggtccctct
    14701 tatccgctgc gccggccgct aggatctcaa caggacccgc cccttcgacg gggatcctgc
    14761 aactacctac cccaagtgct ggaggaagat cggtggcgta tctccagtca aagcgccact
    14821 gcccccatcg gatggaccca tattccccgt ggggagcgaa agttacccgg tccggctcct
    14881 ttgcgctgaa tactttccta tccacgagtg atgcggttct tccggaagcg cagatctcga
    14941 ggtccccttc ccattggaat ctccaccgtc cctctttctt ccttatcagc cctagaagaa
    15001 ccgaggtctt cctccttgaa aggatggata aaaccaggtc cctcccctca aaggcgatga
    15061 gcgactggat ggcctggtca ttcatccttc gaagaaggtc aaggtcccag gatctcaagg
    15121 cgaagggctg ccatctccgg caccaggcgg ccatgcttcg ggactccttt tccctggcgg
    15181 cttccagctc accggcttcc gcggaaaggt ccagcagatg gtccttaaaa cggatgttac
    15241 cctcattgca tagcaagggg aagaaaacat gcctgatctt gtttctaaaa tattttgttt
    15301 caaggttcgt ctcatcttcc atccagggaa cggaagcttc caggagcaac cctcgcagct
    15361 catccctgga aatgcccatg atcgggcgta caaccctttc cctggcccag ggaatgcctc
    15421 tcaaacccct gatcccggtc ccgcggagca ggttcagaaa gaccgtttca accaagtcgt
    15481 cggaggtatg gcctgtcgcg atcaaagggg ccgccgaaca ggaagccacg cgccggagga
    15541 aggcatacct ttcccgcctg cctgcttctt cgagggtctc ccctcttcgt ttcatggaag
    15601 ggatgtcgag gcgttctaca tggcactccg tattccattc acgacagatg cgctccacga
    15661 attccgcatc tctcagggag gtctcctgcc gaaaaccgtg ttcgagatgg gccgcgatca
    15721 ctcttccctt ccacctgaag cggagaagcc agaaaagagc catggaatcg ctgcctccgg
    15781 agacagcggc cacgatcggc ccgtcatggt cccaccagcc ctgatccctg ccggcagctt
    15841 caaaacgccg gaatagtgac cgtgtacctg ggctccatct ttccacgaag ggatggtcct
    15901 tcatcacgag acctctcctc ccttcagcca atgaacattt ctcccggatc aaatactatt
    15961 ttaggactcg actcattgcc tttgcccaac tatccttcaa ggggatagta cggttgaata
    16021 ccgggtggtc cttccggtgc tcttgatccg ctacgaaata acccagcctg aaaaactgga
    16081 accgatccat gggagcagct tcggtgagaa ggggttccac catacaatcc ttgatcacct
    16141 ccagcgaagc ggggttaagg taatcctcgt aatcggtccc ttcttccatt tcatccatcg
    16201 actccagggt gaaaagatga tcatacaggc ggacctcggc tttcacggca tggatggcgc
    16261 taacccagtg aagagtgccc ttgatgcggc gcccatcggg ggcttctccc cccctggatt
    16321 cggggtaata ctcgcatcta acggcgatca cccggccatc gggcccttta tcaaaaccat
    16381 tacattttat aatataggcg tgcttcagtc tcacttcaga ccccggcgaa aggcggaaga
    16441 acttggccgg cgggttctcc atgaaatcct ctctttcgat aagtaattcc ctaccgaagg
    16501 gcacctttct ataggaaggg gtctcagcct ccgggttcac caggacctct atctcctcgc
    16561 tcaaaccctc ggggtaattc tctatgatca gtctcagggg cctcaaaacc gccatagccc
    16621 tgggcgcgac ggcgttcaat tcatccctga tacagtggtg aagaagttcc acatcgacga
    16681 gactgttcgc cttggagacc ccgatctttt tgcagaaagt cctgatcgag gaaggagtat
    16741 acccccgccg cctcaatccg ctgatagtgg gcatcctggg atcatcccac ccggatacga
    16801 aacgattcga aaccagctcc agaagttttc ttttgctcat gaccgtgtag gtaaggttga
    16861 gcctggcgaa ctcgtactgt ctcggcctgg atgggaccga tacattttcg atgaaccagt
    16921 cgtaaagagg ccgatggtcc tcgaattcta gggtacagat cgagtgggtc accccctcga
    16981 tggcgtcctc ataaccgtga gcgaagtcat acatggggta aatgcaccat ttatcccccg
    17041 ttcggtgatg ggactggtga aggatccggt acatcacggg gtccctcatg ttgaggttgg
    17101 gatgggacat atctatcttt gcccttagga cgcattctcc ttcggggaaa tagccttcct
    17161 tcattttgcg aaagagttcc atgttttcct cgacgggcct gtccctgtag ggggattcaa
    17221 cccctggctc cgtgagggtg ccgcgattct gcctgatctg gtcggccgtt tggtgatcga
    17281 cgtaagcctt gcctgcctgg accagttcaa gggcccactc gtgaaactgg tcgaagtaat
    17341 ccgaggcgaa acgtagtttc gcccaatgga aaccgagcca gctcacgtcc ttcattatgg
    17401 actccacgta ttccgtctct tccttgctcg ggttcgtgtc gtcaaatctc aggttgcact
    17461 cgccatcgaa ctgttcggcg atgccgaaat taaggcatat ggatttggcg tgtccgatat
    17521 gaaggtaacc attgggttcc ggggggaagc gggttatcac cttgctcacc cttcccgatt
    17581 cgaggtcttc ccgaatgatc tgctcgatga agctgccgcc tctttcatcc tgatcttccg
    17641 tcatgacgtc gatcacctct ttgggcgaac ccggcctgag aaggacatac agcctccggg
    17701 tcccgggata agcctagtaa cttataatag cctattatta cggagacggt ccgttaattg
    17761 ggaatcaccg ggatcccggg tcgggaggtc tcgaagaatt cgcggccagg tacaaaattg
    17821 cccccggctc aagccagggg ccttaagatc tccgagtggt ggcggtgagt ggaatcgaac
    17881 caccgacact gcgggtatga accgcatgct ctagccaact gagctacacc gcctgaagtt
    17941 ggttgcgggg gcag
//
LOCUS       DAOSRG010000006        17856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_3287_length_17856_cov_10.657379, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000006 DAOSRG010000000
VERSION     DAOSRG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17856
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_17856_cov_10.657379"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="groES"
                     /locus_tag="PLM30_00545"
     CDS             complement(<1..262)
                     /gene="groES"
                     /locus_tag="PLM30_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOR53685.1"
                     /translation="MKLKPLGDRIVVKVLTQEEKTRGGIVIPDTAKEKPQEGEVLAIG
                     SGRVLENGQKLPLEVKKGDKVLFSKYGGTEVKIEGEELLILSE"
     gene            complement(432..1490)
                     /gene="tsaD"
                     /locus_tag="PLM30_00550"
     CDS             complement(432..1490)
                     /gene="tsaD"
                     /locus_tag="PLM30_00550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR53686.1"
                     /translation="MPALNPQGHGPVVLGIETSCDDTGVAVLGFDGRVRSSLLSSQAK
                     DHALFGGVVPELASRKHQEAIIPLLRAALAEAGVEDPSSQVGLVAVTTGPGLMGSLIV
                     GFMTARGLSQAWGCPLVGVNHLEGHIFANRVAHEELETPFLCLIVSGGHTEVILVEGW
                     GDYTLLGRTRDDAAGECYDKVARMLGLPYPGGPEVDRLAASGDPDSFDLPVPLGATGR
                     VEFSFSGLKTAVLWTVNRINSEGRSLPLADICASFQKAAVEALLGKMILASRLTGVGR
                     VCLSGGVSANSALRKAFRDRFGDNVFIPPLELCTDNASMIAAAGLFNFLRSGPSGPDL
                     SPDPSASLTDKITQNEVN"
     gene            complement(1657..2184)
                     /locus_tag="PLM30_00555"
     CDS             complement(1657..2184)
                     /locus_tag="PLM30_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53687.1"
                     /translation="MRKSILFLAAMILLVSAATAFSTDLEVLVKERSVTLYPEGQLLG
                     DLVIGARGKILFVYVDKSLAHAVRGTEMPPEWLSWHSRYWGTDQAKGKALFIIRYEAN
                     KPWTFDPGDIFIGGRRLERGDILTDKAFIAEGDLPSGAEGILSVLVPQESAAPGKVTT
                     MAYLEDSVEWTVPAK"
     gene            complement(2536..2883)
                     /locus_tag="PLM30_00560"
     CDS             complement(2536..2883)
                     /locus_tag="PLM30_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOR53688.1"
                     /translation="MRPHCNLDVWNQAMELAKVIYKATKGFPSEERYGLTGQLRRCAV
                     SIPSNIAEGACRGSRKDFLRFLMIARGSLGELETQCRLAADLGYLQDGNPIYEKADRI
                     FQMLCGLIRSLRD"
     gene            complement(3140..4711)
                     /gene="murJ"
                     /locus_tag="PLM30_00565"
     CDS             complement(3140..4711)
                     /gene="murJ"
                     /locus_tag="PLM30_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOR53689.1"
                     /translation="MSSPGSRMVRHSLRMMAGTFLSRILGLAREVLTAALFGATRQLD
                     AFLVAYTLANLSRRLLAEGALSAAFVPEFTRTLAKDGPASARSLARQIMSVLLAATIL
                     VTAAGMVAAPVLVRVMAPGFDAEGTRAAMSLTRLMFPFLVFISVAALAMGVLNSLDCF
                     FVPAIAPALSNGVFIVFLAIVTLRWGVWGLGCAVLLGGVTQMVLQWAWSAKKGVLLVP
                     AMPRRDNEALSRAMRLFLPYAAGLSLNQLIPLISRMLGSFLEEGAISVLNYADRVLQL
                     PLGLFVIAISQAILPQLSRQLLAGEEDFFQGVREALRYGLFIVLPVALGMILFSGEII
                     HLLFFRAAFGEWAWRGTAGALAMYSLGLPGMACSTILLRALYARGLPREAMIVTAFSV
                     GANVVFSLLLIRPMGINGLALASSLAFSGTALLTWRLLPGQKSAESLFRRDWVIPLAL
                     GLGLMGLLLVCLKILFPYPLYGSILSKSSWMALPLLLGAGAYALVTWKAGSPEWAWIV
                     EAFKGRKPKTVNRES"
     gene            4762..5238
                     /locus_tag="PLM30_00570"
     CDS             4762..5238
                     /locus_tag="PLM30_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOR53690.1"
                     /translation="MKGLTFVWGMGNELMGDDAVGIRAAEMVKTRALPWVRVFLCGVL
                     PENHLAPLREAPPENLIIIDAADCGGEPGETRLLGLRDVEGAALSSHGIPLSVLLAPF
                     EGATRIRIIAIQPAFTGLREGLSPPVETAAARVAEAVCNGTWGEIPPLHSENLSPR"
     gene            complement(5211..5939)
                     /gene="lptB"
                     /locus_tag="PLM30_00575"
     CDS             complement(5211..5939)
                     /gene="lptB"
                     /locus_tag="PLM30_00575"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOR53691.1"
                     /translation="MSNRFLWARSLSKSYKGRTVVSSVDLKVRQGEIVGLLGPNGAGK
                     TTTFYMVLGIVKPDSGRIMIDNDDLTGLPMYIRTRRGIGYLPQEASVFRSLTARENIE
                     LVLQEKGESRRNIVLLTSDLIEEFGLGEVEDIPGYALSGGERRRVEIARSLAMKPSFI
                     LLDEPFSGIDPIAVYDIQQIILRLRTKGYGILLTDHSVRETLAITDRTYLIHRGQILV
                     EGTPSEVAESEAARKFYLGERFSL"
     gene            complement(5936..6604)
                     /locus_tag="PLM30_00580"
     CDS             complement(5936..6604)
                     /locus_tag="PLM30_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53692.1"
                     /translation="MTMDKKAALMILTALMLLAAAAATGADQMQLTSDTLQYDPSGGV
                     VFASGHVKVVGRGAEITSEEGEFDPTGARSLFRKNVLARWPERRLTMDCDEMIVEETP
                     RGQKIIARKVTRFHDPARKVTVRAETVEGFVVGGVFTEFDARGGVVADAVAPDGEPTR
                     VSGGRSRYSRDRQTLEFTGGAVGVQKNRRITADTFIVHLESGKIEAVGNPRMEVDLPP
                     QEVQ"
     gene            complement(6710..7705)
                     /locus_tag="PLM30_00585"
     CDS             complement(6710..7705)
                     /locus_tag="PLM30_00585"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOR53693.1"
                     /translation="MRVALLGATGLVGREMMRTLEARGFPASEVVPLASSRSRGSRVP
                     FRGGELEVLEVNADSFVGVDIAFFSAGADASRRWAPEAKKKGAVVIDNSSAWRMDPDV
                     PLMVPEVNPGAMAGHKGLIANPNCATIQAVMALKPLHDAAGLVRFSVVTFQSVSGTGK
                     EAIEELAGNSGAFLSGEEGAASVYPHDIAFDVLPQIGGFDEDGVSEEEWKMIRESRKI
                     MGIPGLEVRCTAVRVPVFRGHSEAVTAIFERVLTPGEARELLSSAPGVRLLDEPSKGL
                     YPTARGSQGREPVFVGRVRRDTISEKGLMMWVVSDNLLKGAALNAVQIAEALAFR"
     gene            complement(7702..8919)
                     /locus_tag="PLM30_00590"
     CDS             complement(7702..8919)
                     /locus_tag="PLM30_00590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOR53694.1"
                     /translation="MSIEVLKYGGSSLTGEGRIEAVAHQVADLHRKGVSLVVVVSAVG
                     RTTKELIETANGFAGRLNRREVDQLISTGEQQSMALLTMALGEMGIPARSFAGWQAGI
                     RAGGIFTDGRVLSVDPRMVARSVKKGEVAVVAGFQGVTDGGDVITLGRGGSDLTAVAL
                     AAALEAECCRVFTDVEGIFSADPRVVPGAFRLEKVSYGECMEMAAAGARILQARSVEL
                     AWRYGVPLFVGSSFGKGRGTWIMRDDVSEGFVVRSITHDTEVAKVAVLGVPDKPGIAA
                     RVFSSLAGAGVGVEMIIQSIMRGQVNDIAFLVRKEFLGEAIDVCRRTARDIEAQGVAF
                     DTEVGKISLVGAGLSNHPDAPARMFSILAGESVNIDMISSTSMSITCVVSSADVERAV
                     AALHKAFIEEAAV"
     gene            complement(8916..9860)
                     /gene="thrB"
                     /locus_tag="PLM30_00595"
     CDS             complement(8916..9860)
                     /gene="thrB"
                     /locus_tag="PLM30_00595"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOR53695.1"
                     /translation="MNGPSIRIRVPATTANLGSGFDTIGLALSLYNLYDVFDIDEPGA
                     YRMEVIGEGSAELSDPESNLIIKSYERACEEWDLQCPGFSLRCLNAIPLCRGLGSSST
                     AVAGGVMIANAFRKEPLPVEELIPLMVEIEGHPDNIVPACLGGLVVSGAGEGRLRYVK
                     LPPPPGDIFAVVAVPEVRVRTEDARNALPREVSLGDAVFTLNRAAILAASWAAGKWEN
                     LAWAMDDRLHQRFRAKLFPGGEVILEKVKGVPGCLGAAISGSGPSVLAFAKGAPRGVA
                     EAMCRVFREHGVRSRFFVLDVDEAGAVVERVSPGVTRP"
     gene            complement(9857..10897)
                     /gene="thrC"
                     /locus_tag="PLM30_00600"
     CDS             complement(9857..10897)
                     /gene="thrC"
                     /locus_tag="PLM30_00600"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOR53696.1"
                     /translation="MKGILSRYKTLLPINPGTPLVSLGEGETPLIPLETLGEELGLRL
                     FAKYEGANPTGSFKDRGMVLAVAKALEAGAKGLVCASTGNTSASAAAYAARSGLPCTV
                     LLPKGKVASGKLAQALIHGARVVAIRGNFDEALELALALSREMGLAVVNSVNRYRLWG
                     QRSVAWEMTDELGRPPDAVVLPVGNAGNITAGWAGFVQYRRAVKADSVPAMIGVQAEG
                     AAPLALGKPCPAPETVATAIRIGRPVNGKKAAWAVRMSGGKFLAVSDEEILGAQRRLA
                     AHEGVFAEPASCAGIAGLLRYAGQGLIPRGSTVVAILTGNGLKDPATAVAGAGAPLEI
                     DAEVGALREALS"
     gene            11362..11862
                     /gene="greA"
                     /locus_tag="PLM30_00605"
     CDS             11362..11862
                     /gene="greA"
                     /locus_tag="PLM30_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOR53697.1"
                     /translation="MSDVTTTDHIDMTREGYEKLKAELVELRSEGRQEIARLLEEARG
                     FGDLNENAEYHSAKEAQGKLETRIQWLEYQLGKARIVEPEDIDPSKAGLGTTVTLLDL
                     DGGKSLSYTIVGSEESDPAANRISASSPVGQALIGKTVGEELSVEVPKGTRKFRITDI
                     SILPTG"
     gene            complement(11848..>12309)
                     /locus_tag="PLM30_00610"
     CDS             complement(11848..>12309)
                     /locus_tag="PLM30_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR53698.1"
                     /translation="FSFDSAVLVEEYIPGREITVTVWEEREETSLLPVIQIEPRQGFY
                     DYQAKYTPGQTEYLCPAPLAPVERESVGGAAVAAHRSLGCRVFSRVDMRLTREGHPYV
                     LEVNTVPGMTAMSLVPRSAAAMGWGFDELARRIVRSSMELRSAGTGPLTRR"
     assembly_gap    12310..12409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12528..14165)
                     /locus_tag="PLM30_00615"
     CDS             complement(12528..14165)
                     /locus_tag="PLM30_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53699.1"
                     /translation="MRAGAFLLALSFLLFGPLAASAGDGRIVLEADRVEFDQASGFAV
                     ATGAVCISRDLLRVFAPRIEYSAVDNTIEAFSEPGGKKVVLFHGPQRLEGEHLALDMV
                     SGEGLFRNASGGFPAEKGEVYASGSDVTTTRFKEGQRFGSIPGRVQKDLVTGDQVYQW
                     RNVGFTTCPAENPHYQLVSKRLVVIPGFRLVASKPAVYIGGKFLLSYPFDYVIDLSNG
                     SRSQFLPQVMYEGDKGVGVSYGAPLAMGDVNARWKAFLWSGVGLEAVLSVDHRFSDGI
                     VFFADASYTWDGDREEKRFRPSWGVDYEFGGWTGRLWWSRAESVEVEKDLGETFEGLI
                     DRSPEFTLLSPWWTLPGVLGQWRFMATWGDYEVSSEGGTVKSSSSRLGLGAAYEGETK
                     FGNVAPFWGAEYWWYDYGSPGDSQKVMTVRLGLAWPLGPVTMTSLWRKRWVEGGSPMA
                     WDDLSDSEVFYQKAEIPFGEHWSLAVRGGYNLRDSSLDEMYYRLSYDSKCCFRVELVY
                     RDDRVGEDDWAGMVFVLNAFPSHPFFLGPREAGEYNP"
     gene            complement(14162..14713)
                     /locus_tag="PLM30_00620"
     CDS             complement(14162..14713)
                     /locus_tag="PLM30_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53700.1"
                     /translation="MVPESKRRFFLIAAVVLVLLFAVGLVRDLLLGREDVAVSLETPA
                     IIVEGLDVIRQEKGDDWHLRAERAQKSDDLSEAEDIDLFIRSKTGVLWDMTAPRGTIF
                     EDSGGVRLFQVTGHITHPSGELDWNAPVADWDEGGSVWKLPEGLEASDDRLVLVGSRG
                     RITMSGSVVVEEGAVVTWQGTTR"
     gene            complement(14760..15200)
                     /locus_tag="PLM30_00625"
     CDS             complement(14760..15200)
                     /locus_tag="PLM30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53701.1"
                     /translation="MATYLSRGKRVIASIAAGVLLFAGAASAVDLGDLIGVIGGGLIV
                     GALGNQINDFINTVTFNSGVGTDEETKVIPIVSVGSGTSIGAAQVAGPKAEVDKVKAV
                     AELSVSFMGRIGVRVLVPIDSTNPLERFRRVQKVGVSAVINYKL"
     gene            complement(15181..16221)
                     /locus_tag="PLM30_00630"
     CDS             complement(15181..16221)
                     /locus_tag="PLM30_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53702.1"
                     /translation="MKPRIRFAFPLALLLLVMAFAGVSHGQEVDTPEQVQDRIDLLIY
                     AEKGAGGIIRRLEKIETDLFGRELPGSIAERQSGLLNFISNGTLGQPSLIFKAGLAEW
                     AIAHEVRPDLPLASRVTEIERQLEGEAAEGRPLAMRLERVLTLLFPGQVLWQDVNVPA
                     NLVFKATFIDRVSPRNAAAGDVVRLKLEDNLAVEGFLVAPKGSRVIARVDVVKPPRSF
                     GRPSEIKFAFERLEPLGPEAIPVFVGDASALAAKSDTTVATAIGTSAIGAILLGPVGL
                     VGGLLVKGDAPDVAPGTPIYLETASATPVKGYAIPPSLQGLPGSVKPPAPQEGTTHEE
                     FEQPMEGGDLLE"
     gene            complement(16218..17840)
                     /locus_tag="PLM30_00635"
     CDS             complement(16218..17840)
                     /locus_tag="PLM30_00635"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOR53703.1"
                     /translation="MPKYVFVTGGVVSSLGKGITAASLGVLLKRRGFRVSIIKMDPYI
                     NVDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFIDESLSRDNTVTTGKVYSAVISRE
                     RAGQYRGATVQVIPHITNEIQERILKVNNGVDVVISEIGGTVGDIEGQPFLEAIRQMA
                     TRVGRENVLYCHVTLVPYIAAAGELKTKPTQHSVNELRRLGIQPDVIVCRSVSHLGAD
                     IREKIALFGSVPKEAVVEAIDVPSIYKIPLQLHEQRFDSLVMGKLGLEPKQEPDLEDW
                     RAFVKATENPERIVEVAMVGKYVSHKDAYLSVVEALSHAGTANRVRVRIRQVEAEDVE
                     KEGVGLLEGVHGILVPGGFGSRGVWGKIRAAAYAREMRIPYLGICLGMQIAVVEFARN
                     VCGIPSAHSVEMDTNTLDPVIASGPREPGSEDLPGAMRLGLYPCELDPSSLSFDAYGG
                     KKVVEERHRHRYEFNNDYREILSSRGMRIAGVCPERDLVEIIELEGHPWFVGVQFHPE
                     LKSRPVRPHPLFVGFIGAAQERAEENEKEAGK"
BASE COUNT         3398 a         5868 c         5245 g         3245 t
ORIGIN      
        1 gctcgctgag gatgagcagt tcctcgcctt cgatcttgac ttcggtgccg ccgtacttgc
       61 tgaaaaggac cttgtctccc tttttgacct cgagcgggag tttctgcccg ttctccagga
      121 cccttccgga gccgatggcc aggacttccc cctcctgggg tttctccttg gcggtgtcgg
      181 ggatcacgat gccccccctt gtcttctcct cctgggtgag gaccttcacg actatcctgt
      241 caccgagcgg cttcaacttc atttccatac ccctccctgc ggacatatgt tgaaaaaatg
      301 ttagcactca atagcatcga gtgctaaatg accgcgaatg atattaatag gctgggcttt
      361 caatctcaag gggtttgacc atacttcctg ggtaaataac gttgaaaagg acagtatttc
      421 tctattttct cctagtttac ttcattttgc gtgatcttat cggtcagtga tgccgaggga
      481 tccggcgaga ggtcgggtcc cgacgggcca gaccgaagaa agttgaaaag cccggcggcg
      541 gctatcatcg aggcgttatc ggtgcaaagc tcaagggggg ggatgaaaac gttgtccccg
      601 aaacggtctc tgaaggcctt cctgagggcc gagttggccg aaacccctcc ggaaaggcag
      661 accctcccga cgccggtaag ccttgacgcg aggatcatct ttcccagcag cgcctccacg
      721 gcggcctttt ggaaggaagc gcagatatcg gccaggggaa ggctccgccc ctcgctattt
      781 atcctgttca cggtccacag cacagcggtc ttcaagccgc tgaagctgaa ctccacccgg
      841 ccggtcgcgc ccaggggcac cgggagatcg aaggaatcgg ggtcgcccga ggcggcgagc
      901 cggtccacct cggggccacc cgggtagggc aaccccagca tcctggccac cttgtcgtag
      961 cactcccccg cggcgtcgtc cctggtcctc cccaggaggg tgtaatcgcc ccacccttcc
     1021 accaggatca cctcggtgtg gccgccggag acgatcaggc ataggaaggg cgtttcgagc
     1081 tcctcatggg ctacgcggtt cgcgaatata tggccttcca ggtggttgac ccccaccagg
     1141 gggcaacccc aggcctggga aaggcccctg gccgtcatga agcccacgat gagcgacccc
     1201 atgagccccg gccccgtggt gactgccacg agccccacct ggctcgaggg gtcctccacc
     1261 ccggcttcgg cgagggccgc cctcaaaagg gggatgatgg cctcctggtg cttcctcgag
     1321 gcgagttccg gcaccacacc accgaaaagg gcgtggtcct tcgcctggct tgagagcaag
     1381 gaggacctca cccggccgtc aaaacccagg accgcgaccc ccgtgtcgtc gcaactcgtc
     1441 tcgatgccca gcacgaccgg gccgtgtccc tggggattca atgccggcaa ccggggcacc
     1501 cctggcagga accggaacag cccttcgacc ccttgccgag gggcggcccc gcttcgtgga
     1561 tgaaagccct ggcaccgcaa ctcgggcaac gggaggggcg acacccgctc gtgaacctct
     1621 ctccgcagga ggcgcaccgg aaaaggtgtt cggtgctcat ttagccggca cggtccattc
     1681 gacggaatcc tccaggtagg ccatggtcgt gaccttcccg ggcgcggcgc tctcctgggg
     1741 gacaaggacc gaaaggatgc cctccgcacc ggaggggagg tccccctcag cgatgaaagc
     1801 cttgtcggtc aggatatcgc cccgctcgag gcgccttcct ccgatgaaaa tatcgccggg
     1861 gtcgaaggtc cagggcttgt tggcctcgta tcggatgatg aaaagtgctt tacccttggc
     1921 ctggtcggtt ccccagtaac gggaatgcca ggaaagccac tcggggggca tttcggtgcc
     1981 ccgtacggcg tgggccaggg atttgtccac gtagacgaag aggatcttgc ccctcgcccc
     2041 gatcacaagg tcacccagga gttgaccctc cgggtagagc gtgacgctcc gttccttcac
     2101 cagcacctcc aggtcggtgg aaaaggcggt cgccgcggac accagcagga tcatcgccgc
     2161 taaaaacaat atagacttcc tcaaaggtca taacctcctt tgaagtaaaa tccgtaaatc
     2221 ggaaaatccg tgaatcgtaa atcgtaaatc gaaaaaaaac cgtaaaccgt gaatcgttaa
     2281 tcgaaaaacc aaaatcggaa aacgaggatc aaaaccgtgg accgtgaatc ggaaacaaaa
     2341 tccgtaaatc gtgaatcgga aactaaaaac cgtgaatcgt aaatcgtgaa ccgtaaaccg
     2401 gaaactaaaa accgcaaatc gtgaagcggg agtcgaaaag gccaatgctc aaggaacaat
     2461 gaaaaaggca aaagccggga atcagctcgc cattaacgag gagattatcg gggtgatccc
     2521 cgatactgtt cccccttaat ctctcagtga ccgaatcaac ccacaaagca tttggaaaat
     2581 cctgtcggct ttttcataaa tgggattccc gtcttgcaga tatcccagat cggcggccag
     2641 gcggcactgg gtttccagtt ctcccagtga acccctcgct atcatcagga accgaagaaa
     2701 atccttccga gaacctctgc acgcgccttc tgctatgttg cttggaattg agaccgcgca
     2761 gcgtctcaac tgtccggtca acccatagcg ttcctccgag gggaaaccct ttgtggcttt
     2821 gtaaataacc ttagccagtt ccatggcttg attccataca tcaagattac aatgaggtct
     2881 catgcttatg gacaaatcgg aataatcccc cttgatcatt catcgattct cgactcacaa
     2941 tttccggttc acggattttg atttcagatt tacgcctttt gccttggcct tggcttcccg
     3001 gtttccggtt tccggtttcc ggtttccggt ttccggtttc cggtttccgg tttccgattt
     3061 acggttttag gtatccgatt cacgattcac ggatttttcg attcacgatt cacgattcac
     3121 gatttacggc tttttcgatt cacgattcac gatttacggt ttttggtttc cggcccttga
     3181 aagcctcaac gatccaggcc cactccgggc tcccggcctt ccaggtgacc agggcgtaag
     3241 ccccggctcc cagcagcagc ggcagtgcca tccaggagct ctttgaaagg atactcccgt
     3301 aaagaggata agggaacagt attttcaagc aaaccagcag cagccccatc aggcccaacc
     3361 ccaatgccag ggggatgacc cagtcccgac ggaagaggct ctccgcgctt ttttgtcccg
     3421 ggagcagcct ccaggtcagc agcgccgtcc ccgaaaaggc cagcgatgag gccagggcga
     3481 gaccgtttat acccatgggc ctgatcagga ggaggctgaa caccacgttg gcacccacgc
     3541 tgaaggcggt cacgatcatg gcctccctgg gcagaccccg ggcgtagagc gccctgagga
     3601 ggatcgtcga gcaggccatc ccgggaagcc cgagggagta cattgccagg gcgccggcgg
     3661 tccccctcca cgcccattcg ccgaaggcgg cacggaagaa gagcaggtgg atgatctcac
     3721 cagagaagag gatcatcccc agggccacgg gaaggacgat gaagaggccg tacctcaagg
     3781 cctccctcac gccctggaag aagtcctctt cccccgccag gagctgccgg gacaattggg
     3841 ggagtatggc ctgggatatg gctatgacga agagccccag gggcagctgg agcaccctgt
     3901 cggcatagtt caggaccgaa atggcccctt cctccaggaa agaccccagc atgcggctta
     3961 tgaggggtat gagctggttc agggagagcc ccgcggcgta gggtaggaag agcctcatcg
     4021 ccctggagag ggcctcgttg tcccttctcg gcatggccgg taccaggagg accccctttt
     4081 tcgccgacca ggcccactgg aggaccatct gggtcacacc ccccagcaga accgcacagc
     4141 cgaggcccca gaccccccac cggagtgtta caatcgccag gaaaacgatg aagacgccgt
     4201 tgctcagggc gggggctatc gccgggacga aaaagcagtc caggctgttc aataccccca
     4261 tggcgagggc cgcgacggag atgaagacca ggaaggggaa catgaggcgc gtcagcgaca
     4321 tggccgcccg ggtcccctcg gcgtcgaaac ccggcgccat taccctcacc aggacagggg
     4381 ccgctaccat gccggcggcg gtcacgagga tggtggcggc gagaagcacc gacatgatct
     4441 gcctggccag ggaacgggcg gaagcggggc cgtccttcgc tagggtcctg gtaaactcgg
     4501 gcacaaaggc ggccgagagg gccccttcgg ccagcagcct cctggagagg ttcgccagcg
     4561 tgtaggccac gagaaaggcg tcaagttgcc gcgtcgcccc gaagagggcc gccgtcagga
     4621 cctccctggc gaggcccagg atgcggctca ggaaggtccc ggccatcatc ctgagggagt
     4681 gacggaccat cctggaaccg ggggaactca tgtcaggaca ccagccttcg gcgcggggga
     4741 cgcgggaaag ggtgaagggc catgaaaggg ctcaccttcg tatggggcat gggaaatgaa
     4801 ctcatgggag acgacgccgt cggcatcagg gccgccgaga tggtcaagac ccgggcgttg
     4861 ccgtgggtac gggtcttcct ctgcggtgtt ctccccgaga accacctcgc ccctctcagg
     4921 gaggcccccc cggagaacct cataattatc gacgcggccg attgcggcgg tgaaccgggg
     4981 gagacgcgcc ttctcgggct ccgggacgtg gaaggggcgg ccctctcctc ccacggcatc
     5041 cccctttcgg tccttctcgc ccccttcgaa ggggcgaccc gtataaggat catagccata
     5101 caacccgcct tcacggggct cagggaaggc ctttcgccgc ccgtcgagac cgccgccgcc
     5161 agggtggccg aggctgtctg caacggaacc tggggcgaga tcccacccct tcacagcgaa
     5221 aacctttccc ccaggtagaa tttcctcgcc gcttcgcttt cggcgacctc cgagggggtg
     5281 ccttcgacca ggatctgccc ccggtggata aggtaggtcc tgtcggttat ggccagggtc
     5341 tccctgacgc tgtggtccgt gagcaggatg ccgtacccct tggtcctcag cctcagtatt
     5401 atctgctgga tgtcgtagac ggcaatgggg tcgataccgc tgaagggctc gtccagcagg
     5461 atgaaggagg gcttcatggc gaggctgcgg gcgatctcta ccctccgccg ttcgccaccg
     5521 ctaagggcgt aaccgggaat gtcctccacc tcgcccagcc cgaactcttc gatcaggtca
     5581 gaggtcagga ggacgatgtt cctcctggac tcgccctttt cctggaggac cagttcaatg
     5641 ttctcccggg cggtaaggct cctgaagacc gaagcctcct gggggaggta gcctatcccc
     5701 cgcctggtcc ttatgtacat ggggagcccc gtgagatcgt cgttgtcgat cattatcctc
     5761 cccgaatcgg gtttcacaat gcccaggacc atgtagaagg tggtggtctt cccggccccg
     5821 ttcggtccga gaagccccac gatctccccc tgcctgacct tcaggtccac cgaggatacg
     5881 acggtgcgcc ccttgtagct cttgctcagc gaacgagccc agaggaagcg gttgctcatt
     5941 ggacctcctg gggaggaagg tcgacctcca tgcgggggtt ccccacggct tcgatctttc
     6001 cgctttcaag gtgaacgatg aaggtatcgg cggtgatcct gcggttcttc tgtaccccca
     6061 cggccccgcc ggtgaattcc agcgtctgtc ggtcccggga gtatcgcgac ctgccgccgg
     6121 aaaccctggt gggttcaccg tcgggggcga cagcatcggc cacgacgccg ccccgggcgt
     6181 cgaactcggt gaacaccccg ccgaccacga acccttccac cgtctcggcg cgcacggtca
     6241 ctttcctggc cgggtcgtgg aaccgggtca ccttacgggc gatgatcttc tggcccctgg
     6301 gggtttcctc cacgatcatc tcatcgcagt ccatggtaag ccgcctctcg ggccaccggg
     6361 ccaggacgtt cttcctgaac agggagcgcg cccccgtcgg gtcgaactcg ccctcttcgg
     6421 aggttatctc ggcgcccctc ccgacgactt tgacgtgccc cgaggcgaaa acgacgcccc
     6481 ccgaagggtc gtactggagg gtgtcggagg tgagctgcat ctggtcggcc ccggtcgccg
     6541 cggcggcagc gaggagcata agagccgtca ggatcattaa ggcggccttt ttatccatgg
     6601 tcacaggcac cgtcctcaaa aagcgagatg gcggcgcccg tcgggcgccg ccgctatttt
     6661 accttattcc ccgccccccc gggcagcaaa gcaccgaaag ggccgaatat caccggaagg
     6721 cgagggcctc ggctatctgc acggcgttaa gggcggcacc tttcaggagg ttgtcggaga
     6781 cgacccacat catgaggccc ttttcgctta tggtgtccct cctgaccctg ccgacgaaga
     6841 ccggttcccg tccctgggaa cccctcgccg tggggtaaag acctttcgag ggttcatcga
     6901 gcaggcgtac acccggcgcg gaggaaagca gttctctcgc ttcccccggg gtcaggaccc
     6961 tctcgaagat cgcggtgacg gcctcggaat ggcccctgaa aaccgggacc ctgacagcgg
     7021 tgcacctcac ctcgaggccc gggatcccca tgatcttcct ggactccctg atcatcttcc
     7081 actcttcctc gctgacaccg tcctcatcga aaccgccgat ctggggcaag acgtcgaagg
     7141 cgatgtcgtg agggtacacc gaggcggcgc cctcctcgcc ggacaggaaa gcccctgaat
     7201 tccccgccag ttcctcgatg gcctccttgc cggtgcccga cacggactgg aaggtgacga
     7261 ccgaaaaccg cacgaggccg gccgcgtcgt gaagaggctt cagggccatg accgcctgga
     7321 tggtggcgca gttagggttg gcgatcagcc ctttatgccc cgccatagcc ccggggttga
     7381 cctcgggtac catcaggggc acgtcgggat ccatcctcca ggccgaactg ttatcgatca
     7441 cgacagcgcc cttcttcttc gcctcggggg cccatctcct cgacgcgtcg gcgccggcgg
     7501 agaagaaggc tatgtccacc cccacgaagg agtcggcgtt cacctccagg acctccagct
     7561 cgccacccct gaaggggacc ctggaacccc gggagcggga agaggcgagg ggtacgacct
     7621 ctgaggcggg gaaacccctc gcttccaggg tgcgcatcat ttcccggccc acgagacccg
     7681 tcgcgccgag cagcgcgacc ctcataccgc ggcctcttca atgaaagctt tatgcagggc
     7741 ggcgacagcc ctttccacgt cggccgaaga gaccacgcag gtgatggaca tcgaggtgga
     7801 ggagatcatg tcgatattga cggattcgcc tgccagtatg gagaacatcc tcgccggcgc
     7861 gtcgggatga tttgacaggc ccgctcccac gagggagatc ttgcccacct ccgtatcgaa
     7921 ggccacgccc tgggcctcga tatccctggc tgtcctgcgg catacatcaa tggcttcgcc
     7981 caggaattcc ttcctgacga gaaaggctat atcgttgacc tgacctctca tgatgctctg
     8041 gattatcatc tcgacgccca ccccggcccc ggccagggag gagaaaaccc tggccgcgat
     8101 cccgggtttg tcggggaccc ccagcacggc caccttggcg acctccgtat cgtgggttat
     8161 cgatcgcacc acgaacccct cggaaacatc atccctcatt atccaggtcc ccctcccctt
     8221 cccgaaactg gaaccgacga aaagcggcac cccgtacctc caggcgagct ccacgctcct
     8281 cgcctggagg atcctggctc ctgcggccgc catctccatg cactccccgt aactgacctt
     8341 ctcgaggcgg aaagcccccg ggactaccct cgggtcggcg gagaatatgc cttccacatc
     8401 ggtgaagacc cggcagcatt cagcctccag ggcagcggca agggccacgg cggtcaggtc
     8461 cgaccctccc ctgcccaggg ttatgacatc ccccccgtcg gtgacaccct ggaaaccggc
     8521 gacgacggct acttcaccct ttttgacgga cctcgccacc atccgggggt ccacggaaag
     8581 gacccgtccg tcggtgaata tgccgccggc ccttatccct gcctgccagc ccgcgaagga
     8641 acgggcgggg atacccatct cgcccagggc catggtaaga agggccatac tctgctgctc
     8701 accggtggaa atgagctggt ccacctcccg gcggttcaac cgaccggcga aaccgttggc
     8761 ggtctcgatg agttccttcg tggtgcggcc caccgccgat accactacga ccagggaaac
     8821 gcccttccgg tggaggtcgg ccacctggtg cgcgacggct tcgatcctcc cctcccccgt
     8881 cagggacgaa cccccgtact tcaggacctc gatgctcatg gccgggtcac ccccggagaa
     8941 acccgctcaa ccacggcacc tgcctcatcc acatcgagga cgaagaaacg ggacctcaca
     9001 ccgtgctccc tgaatacccg gcacatggcc tcggcaacac cccgcggggc tcccttcgcg
     9061 aaggcgagca cgctcgggcc ggaaccgctt atggcggcgc caaggcatcc cggaacgcct
     9121 ttgaccttct ccaggatcac ctcgcccccg gggaagagtt tcgcccggaa ccgctggtgg
     9181 agcctgtcgt ccatggccca ggcgaggttc tcccacttgc ccgcggccca ggacgctgcc
     9241 aggatcgcgg ccctgttcag cgtgaagacg gcgtcgccca ggctcacttc cctggggagc
     9301 gcgttgcggg cgtcctcggt cctgaccctc acctccggga cggccacgac ggcgaaaata
     9361 tcaccggggg gcggtgggag cttcacgtac cgaagcctcc cttcgccggc gccgctgacg
     9421 acaaggccgc cgaggcaggc tgggacgata ttgtcggggt gaccctcgat ctccaccatc
     9481 agggggatca gctcctcgac gggcaggggc tccttccgga aagcgttggc tatcatcacg
     9541 ccccccgcga cggcggtgga ggaacttccc agcccccggc agagggggat ggcgttgagg
     9601 cagcgcaagg aaaaaccggg gcattgaaga tcccactcct cgcaggccct ctcatagctc
     9661 tttattatca ggtttgattc agggtcggaa agttcggcgc tcccctcgcc gatgacctcc
     9721 atccggtacg ccccgggctc atctatatcg aagacgtcgt agaggttgta aagggagagg
     9781 gcgaggccga tggtgtcgaa gcccgagccg aggttggccg tggtcgccgg gacccggatg
     9841 cggatcgagg ggccgttcat gacaaggcct ccctgagggc ccccacctcg gcgtcgatct
     9901 caaggggcgc tccagcgccg gccacggccg tagcggggtc cttcagcccg ttaccggtga
     9961 ggatggccac caccgtggag ccccggggga tgagcccttg cccggcgtac cgtaataggc
    10021 cggctatccc ggcacaggat gccggctcgg cgaaaacgcc ttcatgggcc gccagccttc
    10081 tctgggcccc caggatctcc tcgtcgctca cggccaagaa cttcccgccg ctcatacgga
    10141 cggcccaggc cgccttcttc ccgttcaccg gcctccctat cctgatggcc gttgccaccg
    10201 tttcaggggc agggcagggt ttcccaaggg cgagcggcgc ggcaccttcg gcctgcacac
    10261 cgatcatggc gggaacggag tcggccttca cggcgcggcg gtattggacg aaaccggccc
    10321 agccggcggt gatgttaccg gcgttgccca cggggagcac cacggcgtcg ggcgggcggc
    10381 cgagttcatc ggtcatctcc caggccacgc tcctctgccc ccagagccgg tagcggttaa
    10441 ccgagttgac caccgccagg cccatctccc tcgagagggc gagggccagt tccagggcct
    10501 cgtcgaaatt acccctgatg gctacgaccc tcgcgccgtg gatgagggct tgggcaagtt
    10561 tccctgaggc gaccttcccc ttgggcagga gaaccgtgca cgggagacct gaccttgcag
    10621 cataggcggc ggccgaggcg gaggtgttgc ccgtcgacgc gcataccagc cccttcgccc
    10681 ccgcctccag cgctttggcg acagccagca ccatccccct gtccttgaag gaccccgtgg
    10741 ggttggcccc ctcgtacttg gcgaaaaggc gcaggccgag ctcctccccc agcgtctcaa
    10801 ggggtatgag aggggtctct ccctccccca gggagacgag gggcgtcccc gggttgatcg
    10861 gcaacaaggt cttgtaacga cttaaaatac ccttcatcct caagctctcc ctccatatct
    10921 tgaaaaaaca aggccccttc ccgtctctac ccgtgagacg gaaaggggct ctttccgcgg
    10981 ttccactccg gttccggcaa gttggcgccg gccctcgtcc ccgctgtcag gggcgggccc
    11041 ccgtcacctt atcccttcgc cagggcttcg gatccagctc cggggtggtc ttcaacgttt
    11101 tcctcgcgaa gggacctccc agcccttggg cccctctctc tggcgcgctg aaaaaacgcc
    11161 tactctcccc ttcatcgcct ttaccttatc caatgaccgc gatacttccg cgaatgtcct
    11221 tctttatagg accccgggct ggatttgtca agcggggtgg ccccgatcac attaaacctc
    11281 accctgttgc ccaaatcttg acagtctgtt aaaatacgcc atcagcggac atcatattcc
    11341 ccccgaaggg aggatcgacc catgtcagat gttacaacga cagaccacat cgacatgacc
    11401 cgggagggat acgaaaaact gaaggcagaa ctggtcgaac taagaagcga ggggcgccag
    11461 gagatagcaa ggttgctgga agaagcccgc ggcttcggag atctcaatga gaacgcagag
    11521 tatcattccg ccaaggaagc ccagggcaag ctggaaacga ggatacagtg gctggagtac
    11581 cagttgggca aggcaaggat cgtggagccc gaggacatcg acccctcgaa ggccggcctc
    11641 gggacgacgg tgaccctcct ggatctcgac ggcggcaaaa gcctcagcta taccatcgtc
    11701 ggctccgagg agtcggaccc tgccgcgaac aggatatccg cgagcagccc cgtcgggcag
    11761 gccctgatcg gcaagaccgt cggcgaggag ctctccgtcg aggtccccaa gggcaccagg
    11821 aagttcagga tcaccgatat atccatccta ccgacgggtt aaaggaccgg tcccagcgga
    11881 cctcaactcc atggaggacc gaacgatcct ccgggcgagt tcatcgaaac cccaccccat
    11941 ggcggccgcg gatctcggca ccaaactcat ggccgtcatc ccaggaactg tgttgacctc
    12001 caggacatag gggtgaccct ctcgggtcag cctcatgtcc accctgctga aaaccctgca
    12061 gcccaaagac cggtgggccg cgacggcggc tccccccacg ctctcccgtt ccacgggcgc
    12121 caggggcgcg gggcagaggt attccgtttg tcccggggtg tacttggcct ggtagtcata
    12181 aaaaccctgc cggggttcta tctggatcac ggggagcagg gaggtctcct ctctctcctc
    12241 ccacacggtc acggtgatct ccctcccggg gatgtattcc tccacgagga ccgcactgtc
    12301 gaaggagaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccaggaagcc
    12421 cgcgacctcg agggcggagc atagcgccct gccgctggag agggaaacct ccctttcggg
    12481 actgtccccc ccgaaaagga cggccaccct ggtgggctct gcgggtatca cgggttgtac
    12541 tccccggcct ctcgcgggcc caggaagaag gggtgacttg gaaaggcgtt caggacgaat
    12601 accatcccgg cccagtcgtc ctcgccgacc cgatcatcgc ggtagaccag ttcgaccctg
    12661 aaacagcact tgctgtcgta actcaggcgg tagtacattt cgtcgaggct gctatcccgg
    12721 aggttgtagc ctcccctgac ggccaggctc cagtgttccc cgaagggtat ctccgccttc
    12781 tggtagaaga cctcgctgtc ggagaggtcg tcccaagcca tgggcgaacc tccctcgacc
    12841 catcgtttcc tccaaaggct cgtcatggtg accggaccca ggggccaggc aaggcccagc
    12901 ctgacggtca tgaccttctg gctgtcgccg ggcgacccgt agtcgtacca ccaatactcg
    12961 gccccccaga aaggggcgac gttgccgaac ttcgtttcac cttcgtaggc agctccaagg
    13021 ccgagcctcg atgaactaga cttcaccgtg ccgccctcgg aagagacctc gtaatctccc
    13081 caggtcgcca tgaagcgcca ttgccccagc acgccgggga gggtccacca gggtgataac
    13141 agcgtgaatt cggggctcct gtcgatgagg ccctcaaaag tctcgcccag gtccttttcc
    13201 acctcgacgc tctccgcccg ggaccaccac aaccttcccg tccatcctcc aaattcgtaa
    13261 tccacgcccc aactcgggcg gaaccttttt tcctcacggt cgccatccca ggtgtaggag
    13321 gcgtcggcga aaaaaacgat cccgtcgctg aaccggtgat caacggagag gacggcctcc
    13381 agccctaccc cggaccaaag gaaggccttc cagcgcgcgt tgacatcgcc catggccagg
    13441 ggagccccgt aggatacgcc cacacccttg tcgccctcgt acatcacctg ggggaggaac
    13501 tggctgcggc tgccgttcga aaggtcgata acatagtcga agggatagga gagcaggaat
    13561 ttccctccta tgtagacggc cggcttcgag gcgacgagcc ggaaaccggg gatgaccacg
    13621 agccgtttcg agaccagttg gtagtgggga ttttcggcgg gacaggtggt gaaaccgacg
    13681 ttcctccact ggtagacctg gtcccccgtc acgaggtcct tctggaccct gccggggatc
    13741 gacccgaagc gctgcccctc cttgaaacgg gtggtggtca cgtcggagcc cgatgcgtat
    13801 acctcacctt tttcagccgg aaagccgccc gaagcgttcc ggaagagccc ctcgccggag
    13861 accatgtcga gggccaggtg ctccccttcc agcctctggg gaccgtgaaa gaggaccacc
    13921 ttcttcccgc cgggttccga gaaggcctcg atggtgttat cgacggccga atactcgatc
    13981 ctgggggcga aaaccctcag caggtccctg ctgatgcaaa ccgcccccgt ggcgacggca
    14041 aaacccgagg cctggtcaaa ctcaaccctg tccgcctcca gcacgatccg gccgtcgccg
    14101 gccgacgccg ctagggggcc gaagagcaaa aatgaaagcg ccaggagaaa agcgcccgcc
    14161 ctcatcgggt tgtcccctgc catgtgacca ccgctccttc ctccacaacg acggagccgg
    14221 acatggttat ccggccccgg ctccccacaa ggacgaggcg gtcgtccgag gcttcgagac
    14281 cctcggggag cttccagacc gaaccccctt cgtcccagtc ggcgacgggc gcgttccagt
    14341 ccagttcccc cgaggggtgg gtgatatgcc ccgtgacctg gaaaagcctg accccgccgg
    14401 aatcctcaaa gatggtcccc ctcggggcgg tcatatccca caggaccccc gtcttggacc
    14461 ggatgaacag gtctatatcc tccgcctcgc tgaggtcatc gctcttttgc gccctttcgg
    14521 ccctcagatg ccagtcatcc cccttttcct gcctgatgac gtcaaggccc tccactataa
    14581 tggcgggcgt ctccagggac acggcgacat cttctcggcc caggagaagg tccctcacca
    14641 gcccgacggc gaaaagcaac accagcacga ccgcggcgat caggaagaag cgccgcttgc
    14701 tttcaggaac caccgcactc cccccgttcg ggccgaagca atgagataaa ggacgccaac
    14761 taaagcttgt agttgatgac cgccgagacc cctaccttct ggaccctgcg gaatctctcc
    14821 agcgggttag tggagtcgat gggcacgagc accctgaccc ctatccggcc catgaagctt
    14881 accgaaagtt ccgccacggc cttgaccttg tccacttccg ccttggggcc cgccacctgg
    14941 gccgccccta tggacgtacc gctcccgacg gacacgatgg gaatgacctt ggtctcttca
    15001 tcggttccca cgccgctgtt gaaggtgacg gtgttgatga agtcgtttat ctggttcccc
    15061 aaggcgccta cgatgagccc tccccctatc acaccgatga gatcccccag gtcgacagcc
    15121 gaggccgctc cggcgaagag cagcaccccc gcggctattg acgcaatgac ccttttgccc
    15181 ctactcaagt aggtcgccac cctccatggg ttgttcgaac tcctcatggg tggtgccttc
    15241 ctgcggggca ggcggcttga cacttcccgg gagcccctgg agcgacggtg gaatggcgta
    15301 acccttcacg ggcgtggccg aagctgtttc caggtagatc ggcgttccgg gagccacgtc
    15361 gggggcgtcc cccttcacga gcaacccacc cacgaggccc actggcccga ggaggatcgc
    15421 tcctatggcg ctcgtcccta tggcggtggc cacggtagtg tcggatttgg cggccagggc
    15481 ggaagcatcg ccgacgaaga cgggtatcgc ctcggggccg aggggttcca gcctttcgaa
    15541 ggcgaacttt atctccgatg gcctgccgaa actccgggga ggtttaacga cgtccaccct
    15601 ggcaatgacc ctcgaaccct tcggcgccac caggaagcct tccacggcga ggttatcttc
    15661 gagcttcagg cggaccacgt ccccggcggc ggcgttcctg ggagataccc ggtcaatgaa
    15721 ggtggccttg aaaaccaggt tggcggggac gttgacgtcc tgccaaagga cctggcccgg
    15781 gaagagcagg gtgagcaccc gctcaagcct catcgccagg gggcgcccct cggccgcctc
    15841 gccctcgagt tgccgctcta tctcggtcac cctgcttgcg agagggaggt caggcctgac
    15901 ctcgtgggcg atggcccact cggcgagccc cgccttgaat atcagcgagg gttgacccag
    15961 cgtcccgtta cttatgaagt tcagcagccc cgactgccgt tcggcgatgc tgcccggcag
    16021 ttcccttccg aaaagatcgg tctcgatctt ttcgagcctc cggatgatcc ccccggcgcc
    16081 cttttcggcg tagatgagga ggtcgatccg gtcctggacc tgctccggtg tgtcaacttc
    16141 ctgcccatgg gaaacacccg caaaagccat gaccaggagc aggagcgcga ggggaaacgc
    16201 aaaacggatc cttggtttca ttttccggcc tccttttcat tttcttccgc cctttcctgg
    16261 gcagccccga taaaacctac gaaaagcggg tggggacgca ccggcctgga tttgagttcg
    16321 gggtggaact ggacgcccac gaaccagggg tggccctcca gctcgatgat ctcgaccagg
    16381 tcccgctccg ggcacacgcc tgctatccgc atccccctgg aagacaagat ctccctgtag
    16441 tcattgttga attcgtagcg gtgcctgtgc ctttcctcga cgaccttctt cccgccataa
    16501 gcatcgaagg aaagagagga tgggtccagt tcacagggat aaaggcccag ccgcatggca
    16561 ccaggcaggt cttcagatcc cggttccctt ggccccgagg ctatgaccgg gtccagcgtg
    16621 ttagtgtcca tctccacgct atgagccgag gggatgccgc agacattccg ggcgaactcc
    16681 actacggcga tctgcatccc aaggcagatg cccaggtagg ggatcctcat ctcccgggcg
    16741 taggcggccg cccggatctt cccccagaca cccctggaac cgaatccccc tggtaccagg
    16801 ataccgtgga cgccctccag gagaccgacg ccttccttct cgacgtcctc agcctccacc
    16861 tgacggatcc ttaccctcac cctgttggcc gtaccggcgt gggagagggc ttcgacgacg
    16921 ctcaggtacg cgtccttgtg gctcacgtac ttacccacca tggccacctc gacgatccgt
    16981 tcggggtttt cggtggcctt cacgaaggcc ctccagtcct ccaggtcggg ctcctgcttc
    17041 ggctccaggc ccagttttcc catcacgagg ctgtcgaagc gctgttcgtg aagctggagg
    17101 ggtatcttgt agatcgacgg gacgtcgatc gcctccacga cggcctcttt ggggacgctg
    17161 ccgaagaggg cgatcttctc cctgatgtcg gctcccaggt ggctgaccga ccggcagacg
    17221 atgacatcgg gctgtatccc gaggcgccgc agttcgttga cgctgtgttg ggtcggcttg
    17281 gtcttcagtt caccggcggc ggcgatgtag gggacgaggg tgacatggca atagaggacg
    17341 ttctcgcggc ccacgcgggt cgccatctga cggatcgcct ccaggaaggg ctgtccctcg
    17401 atgtccccga cggtcccgcc gatctcggag atcacgacgt cgacgccgtt gttcactttg
    17461 aggatcctct cctgtatctc gttggtgata tggggtatca cctggaccgt ggcaccgcga
    17521 tactgccccg cccgctccct ggagatgacc gcggagtaga ccttcccggt ggtcaccgtg
    17581 ttgtccctcg agagggactc atcgataaaa cgctcgtagt ggccgaggtc caggtccgtc
    17641 tcggccccgt cgtcggtgac gaagacctcg ccgtgctgga aggggttcat cgtccccgcg
    17701 tccacattga tgtaggggtc cattttgatt atggataccc tgaaccccct tcgcttgaga
    17761 aggaccccca gcgaggcggc ggtgatgcct ttccccagcg atgaaacgac accgcctgtg
    17821 acgaagacat acttgggcat acagttcccc ccggaa
//
LOCUS       DAOSRG010000007        17099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_3454_length_17099_cov_8.697137, whole genome shotgun sequence.
ACCESSION   DAOSRG010000007 DAOSRG010000000
VERSION     DAOSRG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_17099_cov_8.697137"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLM30_00640"
     CDS             complement(<1..472)
                     /locus_tag="PLM30_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOR53704.1"
                     /translation="MEGKKTGVDAVHSGDRVFEGVARPKVPPIYASSVYSFESFSDLE
                     DVFDGKKPGYIYARMGHPNASLLEETLSALEKTEAALVFSSGMAAITTAFLSALEPGD
                     HVVADRVIYGGTFSFIDGFLRNWGVEVTLVDTLDPDQVRKALLPRTKVIYLETIS"
     regulatory      689..807
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            889..1713
                     /locus_tag="PLM30_00645"
     CDS             889..1713
                     /locus_tag="PLM30_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR53705.1"
                     /translation="MKRSLRILFFSVLVLSIAAYSAFSAEVLRMATTTSTDNTGLLDY
                     LAPEFLKDTGIELQWVSVGTGKALELGKNLDVDVLLVHAPEVEKQYVQDGYGVNRRQV
                     MYNDFIIIGPPSDPAKIKGKTVKEAFISIATKGAPFASRGDKSGTHVLELDLWERFGY
                     GQPEKENWYIQTGQGMLDTINIAAEKVGYTLTDRGTFIKYEDNYKGNPPLVILVEGDE
                     ALFNQYSVIAVNPVRGDHLRYDLALKFSDWMRSDKVQKMIGEFSLLGKPLFVPNAK"
     gene            1865..2560
                     /locus_tag="PLM30_00650"
     CDS             1865..2560
                     /locus_tag="PLM30_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR53706.1"
                     /translation="MHYLSEGFKAAFAILLSGQEETFNAIFTTLKVSTFSILLTLAAG
                     IPLGFILGYYSFPGKHACRAISDTLMAIPTVVVGLLIYAFISRSGPFGGLGLLFTLRG
                     VAIGQFFLGLPVVVSLTASATEQMEDSTRLTLMTLGASPGQLAASSLWEGRYAVVAAA
                     MTAYSRIISEVGVSMMIGGNIKWRTRTITTAIALETGKGQFAEGIALGIVLLSIAFIV
                     NGAVSVARKRATS"
     gene            2557..3579
                     /locus_tag="PLM30_00655"
     CDS             2557..3579
                     /locus_tag="PLM30_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR53707.1"
                     /translation="MTNIYELEDITQSYAGRTVLEVSELKIERGSILGITGPNGCGKS
                     TLLRILAFLERPRSGQLLFKSIPVRDINEQLRREATLLLQETRLLKRTVFENVVYGLK
                     ARGIKEGLTRKVHSALEMVGLDPGDFAGRHWFQLSGGESQRVALASRLVLRPEVLILD
                     EPTASIDANSARIIARAIVDARDNFGASIVLVSHDIEWSYGAADEVISMFSGKIRSKN
                     IENIIQGPWEEGNDGTVLRYLEDGQVITAAGKPGNKYSMGLLDPGDIILSLDYPGNSS
                     ARNTLRGTVTRMNLEDEGRSILVTVNIQDTVLTSRITKGSLSRLGIVPGSTVILVFKS
                     NSIRWA"
     gene            complement(3603..4091)
                     /locus_tag="PLM30_00660"
     CDS             complement(3603..4091)
                     /locus_tag="PLM30_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53708.1"
                     /translation="MRILFFLSMSGAAITFALFYTWCIQKPVMTVMRFFQGEDLPEEA
                     PYGETEKLPPAVLPLVWYPLRIVFFFGETYILAAWGAYCILRTLEAMQKAGIEKGPAF
                     HIPAFIACVGALGYLARKEPRKDILTIIQSCIGMGSYLVFVLNRAALMTYYPWLVDFF
                     AK"
     gene            complement(4445..5617)
                     /locus_tag="PLM30_00665"
     CDS             complement(4445..5617)
                     /locus_tag="PLM30_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53709.1"
                     /translation="MNEKIQIEEVLERKGEKAKIRVSGRVFHPSGGGQPGDSGCLESE
                     SFKARVLDCRTSNEGDVLDIVVKEGEIPLGRPVDATVDFERNRRFSRMHTAEHILSRT
                     LENQLPGLDVQKVSIGEKESVVFMTYPGDIAWDDLFRAEEAANRVIAADLPVGVKRVS
                     RERGDSFEGVKIKWDRIDEREVTIVTIGEFDSMACSGSHVGSTSEVGGLIVTGFKGGN
                     GQWEVKFTVDRQETLQRHSRITRVLSREIGCEEEDLPSVVARLREERREATRKLDKVR
                     KYIEIPWEDIGETPGKLYFFAIENFPPELASSGVKRKIEKETGCVVGFLSREESGEAA
                     FMLAAGKDLQVDLRVFLEEADTLQARGGGAADWVQGRAGNGSPSEWRRFLVSIVDK"
     gene            5704..5991
                     /locus_tag="PLM30_00670"
     CDS             5704..5991
                     /locus_tag="PLM30_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOR53710.1"
                     /translation="MGQEKCVRVIITGTVQGVGFRWRARQAALSLGLSGWIKNRLDGG
                     VEAVFQGPPESVDQMIEWIQVGPPGAVVREVMYLHCDPDLGRSDFLILRST"
     gene            complement(6007..7248)
                     /locus_tag="PLM30_00675"
     CDS             complement(6007..7248)
                     /locus_tag="PLM30_00675"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOR53711.1"
                     /translation="MEYKGKVILAYSGGLDTSVAIKWLSDQGYDVVTLTADVGQILDT
                     EEVKEKALKVGAVNAYVMDLRQEFVETMVWPALKANALYQGKYPLNSALSRPIIAKYL
                     VWVAGMEGAVAVAHGCTGKGQDQVRIEVCSNALDPELKVIAPVREWHFSRDAEIAYAK
                     ENGIPVPVTAESPYSIDGNLWGRSIECGPLEDPWTEPPSDAFALTVDPWEAPDGQEYI
                     EITFKKGIPVAINNTIMDGVALIAELNRIAGAHGVGRIDMIEDRLVGFKSREVYECPA
                     TVCLLQAHQALESMTLTKDVIAAKADLSLKYAELTYTGFWFSPLKKALDAFFDSIQEN
                     VSGTVRVRLFKGASVVVGIKAEKSLYVESMATYSEGDAFDHEAAAGFIKIWGLPVKLW
                     RQVHPEVETKVPELKVVGGAK"
     gene            complement(7779..8555)
                     /gene="surE"
                     /locus_tag="PLM30_00680"
     CDS             complement(7779..8555)
                     /gene="surE"
                     /locus_tag="PLM30_00680"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOR53712.1"
                     /translation="MRILVSNDDGAFAPGIVTLVRKLHDQGYRPCVVAPDRERSSVGH
                     AITLHKPLRLRSIQGGGYPEHLEAFSCDGTPSDCVVLGLEAVAPDSEMVLSGINKGPN
                     LADDLTYSGTVSAAMEGLFLGKPSIAVSLNCRPDDEEEHYSTAAEAVVFILGRVREHP
                     LPKNILLNVNVPNVPVSEVQGYRITRKGVRVYREKVTPLKDPKGRSCYWISGKPEDVS
                     EDGSDIGAVVEGYVSITPIHMDMTHYPSITALREAGFEDY"
     gene            complement(8581..>8823)
                     /locus_tag="PLM30_00685"
     CDS             complement(8581..>8823)
                     /locus_tag="PLM30_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOR53713.1"
                     /translation="LGRFVVLMILINLGLGVFNLVPIPPLDGSKLLSLFLPPSWLRGF
                     FWLERYGIFILMVLLALGVVQMLITPMIMTLFHLIL"
     assembly_gap    8824..8923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9071..11710)
                     /locus_tag="PLM30_00690"
     CDS             complement(9071..11710)
                     /locus_tag="PLM30_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR53714.1"
                     /translation="MKVITTHIGADFDSLASMVAAAKLYPGARACFSGSAGRNVREFL
                     KRTPGRWEILTPSQIDMKEITMLIVVDARSPARIASFAGILGRPGLEVHVYDHHAAVQ
                     EEIEASFSLIEPVGATTTILVAKLLEKGIPISPQEATLFAMGIYEDTGALTFGATTEK
                     DFRAILNLKNLGADMTAIPAHIELSLDSSERKIQDKMIENARERYINGAKVVLSSMTS
                     STYVEGLSLFVHRLRDYFDADVALAAVQMEKRVYVVARGREEVLDVAGLLSPLGGGGH
                     SQAAAVTLTGVTPQEVLADLEVRLERSIQPLVTVGEVMTSPVMVISPEKSVEDAYRLM
                     IRYGHSALPVALEGRVRGIITRKDLDRATTHGLGAAKVEDYMTEDPLSIGPGAAVAEA
                     HRIMVSRNIGRLMVTRDRRLMGIVTRTDILRALFPRSLPAETRAVAPEMPWVENISGL
                     MASLLNDPVSSLLKRMGERATSMGMKAYVVGGFVRDLLLGRLNEDLDIVVEGDAVAFL
                     ESWVRNGARVAIHQRFRTGTVIFPDGRKVDVATARREFYEYPVAQPQVSIDSIKHDLY
                     RRDFSVNAMALSINAEDWGTLVDYFGGRRDIKDRLLRVLHNLSFVEDPTRVIRGIRLE
                     QRLGLRIEDNTLRLLENCIQGGLLTLLSGVRLRSEIEPLFQEKNVLAQIKRLNSVGFW
                     GAVFPGVVVDKGALRATRRLEVFKKRLTRDIPDMGNRQWLFFLMALLTDSTDTVALQV
                     LDRLNLSPAERDLIMKGLFELGPAEHRLGGRGQKKPSNIFMFLKEFNPITAFYWAAAT
                     ERWRVRRRILLYLTRLQKVEPMLVGRELLEMGYEEGPLIGSILSRLQMARLDGEVETR
                     EDEIEWVRDNFHLSLLGPERAKV"
     gene            complement(11707..12597)
                     /gene="coaE"
                     /locus_tag="PLM30_00695"
     CDS             complement(11707..12597)
                     /gene="coaE"
                     /locus_tag="PLM30_00695"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOR53715.1"
                     /translation="MLVIGLTGDVGAGKSTVSSAWGSQGARVISADPIVAELWRRGDL
                     VTLAVERWGRSVLTAAGTLDHGAVSRVIFSDAKEYRWACGIIHPLVREEMKRRIEDLD
                     GWVVAEIPLLFENGVPEWVDLTVYVEASEEDRVRRNAFRGWDREELHKRERWLLEPES
                     KKTRADLIISNDGTLDELEEKTRDLGSRFSSLSSLARVCFVSVTPVVSCRLFRELSRD
                     ERVIEVGIASQEECEVAGTDRGGPDLIVTAFVGSCDLEGIKALAEKIGGVGIFVRSSQ
                     PEGRRFPKEVLLRAMKVEKC"
     gene            complement(12557..14533)
                     /locus_tag="PLM30_00700"
     CDS             complement(12557..14533)
                     /locus_tag="PLM30_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR53716.1"
                     /translation="MNRKDDPSLIASLNDSQAEAVRYCSGPLLVLAGAGSGKTRVLAH
                     KFAWLVSEHGLEPRSIMAVTFTNKAAREMRERINRLLGVDLAGLEIGTFHSWGLRFLI
                     RNHPDLERITGRRAATVFDRADTRSLVKGIMKELDIDPRQYEPGWVVERLCRAKSEGD
                     PRTLDIGELDFPIGPIYSKYQKGLARQGAVDFEDLLVLPLHLLVNDSNVRERERERID
                     WILVDEYQDVNRPQYLLLKALSSGRERIMVVGDPDQSIYGWRGADMNMILNFEKDFPD
                     ARVFVLEQNYRSTPNILGGANGVIRNNFRRKPKKLWTSRSGGEKIHVLLAGNERVEAN
                     FITEEVLRLRGLGFRFGDMAILYRINAMSRLYEEVFLRNDIPYRIVRGTAFYERREVK
                     DALSFMRLAVNPFDEAALERIGNMPSRSLGQKSLERLKDHIHASGEQGIALWAKLSKN
                     GAELPGKAGKGVMELGAHMVEINRRSGDIPSVIDYILAGIGYQGELTRMDPEGWGERV
                     DNIRELLSVTSSAEGLERMLAEIALVTDQDLAPDEAESVSLMSLHAAKGLEFPVVFMV
                     GLEEALFPHYRCLDDPDDLEEERRLCYVGMTRAEERLYLTGAGSRVLFGTIQRNGFSR
                     FLWEIPDEFKETGEMSGEEASYAGDRSYGRRWGW"
     assembly_gap    14647..14746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14834..15172)
                     /gene="hypA"
                     /locus_tag="PLM30_00705"
     CDS             complement(14834..15172)
                     /gene="hypA"
                     /locus_tag="PLM30_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOR53717.1"
                     /translation="MHELSLVGAVVESLEEMAREKGWKKVTKVTLRIGRMRQVIPEVM
                     IFSFSVVTEGTIMEGSSLEILEVPLMSRCSFCGEEWEGLSFQCPRCGSDQVETASGME
                     MDLESVEVEE"
     gene            complement(15603..16145)
                     /gene="raiA"
                     /locus_tag="PLM30_00710"
     CDS             complement(15603..16145)
                     /gene="raiA"
                     /locus_tag="PLM30_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOR53718.1"
                     /translation="MDVRFVTRNVDLQDELRDYMERKLAKLEKFFDRILDTQVEVSFN
                     RGMKVVEITADVNGVIMRGEDYASDVRKAFDKCLKNIERQIKKHKSFLKEKARMKTAE
                     VSFEIEGFVNEIAEPEEKKEGIVKTKKFPFRPMTPEEATMQMGLLGHTFFVFRNADDG
                     KVNVVYRRKDGGFGLLIPEE"
     gene            16346..>17099
                     /locus_tag="PLM30_00715"
     CDS             16346..>17099
                     /locus_tag="PLM30_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR53719.1"
                     /translation="MKTSVRASRMEPSATLAVVNKAKALKYEGKPVISFGAGEPDFDS
                     PAAAASCAERAIKEGKTHYTQSNGILELREAVASYYSDRFGLDYPADQVIIGPGAKPL
                     IYGSLASLVDPEDEVLVFSPAWVSYVEQIRLLDGRASIVDTVTTGNVPLASKVAEAVT
                     PRTVGMLINTPNNPTGAVYGEETLREIAEIALEHDLWIIYDEIYERLVYGNSTHLNIA
                     TLVPEVADRTIQINGVSKAFAMTGWRIGYALAP"
BASE COUNT         3610 a         5125 c         4381 g         3783 t
ORIGIN      
        1 ttgagatggt ctcaaggtag atcaccttgg tcctgggaag gagggctttc cttacctggt
       61 cagggtcaag ggtatccaca agggttacct caacccccca atttcttaag aacccatcga
      121 taaatgagaa ggtccccccg taaatcacgc ggtcggccac cacgtggtcc cccggctcca
      181 gggccgaaag gaaagcggtg gtaatagccg ccatccccga ggaaaaaacc agggcggctt
      241 cggtcttctc aagggctgaa agggtttcct ccaggaggga ggcattgggg tgacccattc
      301 tggcgtagat gtaccccggt tttttaccgt cgaacacatc ctccaggtct gaaaacgatt
      361 caaaagaata aactgaactg gcgtaaattg gagggacctt cggccgcgcc acaccttcga
      421 aaacacggtc ccccgagtgg acagcgtcca cgccggtctt tttgccttcc accttctact
      481 cccccgtttc acttttcact tgtgaaaata ttatatcccc tgttatcgcg aagacctacc
      541 gcctcgggcg gcggctgtcc ttcactccgg cctcacccgg aggcaccatc gtcggatcct
      601 ctcttttctt tcttcaatga ccctggcttc actaatcctc ctctatcgcc ggatcccttt
      661 accttccttc ctttacatgg tatatttccg ctaacaaaac ctccgagccc taccgcctaa
      721 aggtatttcc cacggcggcg ggccgaaggg cgttcccgga aacaggaacg cctcccgcgt
      781 ttggaaagga ggaaggagtg gccggcctac cggaccgtct cccctcaacg cggggagacg
      841 gtttttttat gaaaaatgcc cccacttcaa ctgaaggagg ttgttgcaat gaaacgttct
      901 ttgaggatcc tcttcttttc agttctcgtt ctgtccatcg cagcatattc cgctttttcc
      961 gccgaagttt tgcgcatggc cactacgacc agcacggaca acacgggact cctggattac
     1021 ctggccccgg agtttctcaa ggatacgggg atcgaactcc agtgggtttc agtagggacc
     1081 gggaaggccc tcgaattggg taaaaatctc gatgtggacg tcctccttgt ccacgccccc
     1141 gaagtggaaa agcagtacgt ccaggacggc tatggggtca accgccggca ggtcatgtac
     1201 aacgatttta taataatagg tcccccttcc gaccccgcga agatcaaggg caaaaccgtc
     1261 aaagaggcgt ttatctctat cgctaccaag ggggctccct tcgccagccg gggcgataaa
     1321 tccggaaccc acgttctgga actagacctt tgggagagat tcggatacgg tcaaccggag
     1381 aaggagaact ggtacatcca aaccgggcag ggcatgctag atacgatcaa catagcggcc
     1441 gagaaggtcg gctacacctt gaccgacagg ggcaccttca ttaagtacga ggacaattac
     1501 aagggcaacc cccccttggt gatactggtc gagggcgatg aagcgctctt caaccagtac
     1561 agcgttatcg ccgtcaaccc ggtaagggga gaccatctcc gttacgacct ggccttaaaa
     1621 ttttccgact ggatgcgctc cgacaaggtc cagaagatga tcggggagtt cagccttcta
     1681 ggcaaacccc tgtttgtccc taacgcgaag taaccgggct ttgccaatca gcaggggacc
     1741 ccgcatcctc cccacccccc tggggatctt gcggggttcc tttttacaag aaccaggagg
     1801 ccggcccaag gctcgtcgga gacttccaag gtaggataca aggagaagaa aactggaggc
     1861 ggcaatgcat tatctgtccg aagggttcaa agcggctttt gccatccttc tttcaggaca
     1921 agaggagacc ttcaacgcca tttttacgac cctgaaggta tcgacctttt cgatcctcct
     1981 caccctggcg gcggggatac ccttgggatt catcctggga tactattcct tccccggcaa
     2041 acacgcctgc agggctatat cagatacctt gatggcgata cccacagtag tcgttgggtt
     2101 gctgatctac gccttcatct ccaggagcgg ccccttcggg gggttgggcc tccttttcac
     2161 cttgcggggg gtcgccatcg gccaattttt cctcgggctc ccggtggtgg tgtccctaac
     2221 cgcatcggcc acggaacaga tggaggattc cacccgcctc accttgatga cgctgggggc
     2281 ttctcccggg caactggctg ccagctccct ctgggagggt cgatacgccg tcgtggcagc
     2341 ggccatgacg gcgtacagca ggatcatctc cgaggtgggt gtctccatga tgatcggagg
     2401 caacattaaa tggagaaccc ggacgatcac caccgccatc gcccttgaga cgggtaaggg
     2461 ccagttcgcc gaggggatag ccctgggcat cgttctgctg tccatagcct ttatcgtcaa
     2521 cggggccgtt tcggtggcaa gaaagagggc caccagttga ccaacatcta cgaactagag
     2581 gatatcaccc agtcctacgc cggacgcacc gtacttgagg tctcagagct gaaaatagaa
     2641 aggggctcca tcctggggat taccggtccc aacggttgtg ggaaaagtac actcttgagg
     2701 atactggctt ttcttgaaag gccccggagc gggcagcttc ttttcaagag cataccggtg
     2761 cgtgacatta atgaacagtt gagaagagag gccacgctcc ttctccagga aaccaggctt
     2821 ctaaagagga cggtttttga aaacgtggtt tacggcctca aggccagggg catcaaggag
     2881 ggtcttaccc gaaaggtgca ttcggctctc gagatggtcg ggctcgatcc aggggatttc
     2941 gccggcaggc actggttcca gttatccggt ggagagtcac aaagggtggc cttggcttca
     3001 aggctggttt taaggccgga agtattgatt ctcgatgaac ctacggccag tatcgatgcc
     3061 aacagcgcga ggatcatcgc ccgtgccatc gtggacgcca gggataattt cggagcatcc
     3121 atcgtgcttg tgagccatga catcgaatgg tcctacggcg ccgccgacga agtgatctcc
     3181 atgttttcag gcaagatccg atccaagaat atcgaaaaca tcatccaggg tccctgggaa
     3241 gagggaaacg atggtaccgt cctcaggtac ctggaggatg gccaggtcat aacggcggcg
     3301 ggaaaaccag ggaacaaata ttccatgggc ttactggatc ctggtgatat tatcctttcc
     3361 ctggactacc ccggaaattc ttcggcaagg aacaccctga ggggaaccgt caccaggatg
     3421 aacctggagg acgagggacg aagcattctt gttacggtga atatccagga tacagtcctc
     3481 acctccagga tcacgaaggg atcgctctcc aggcttggca tcgttccggg gtcaacggtg
     3541 atcctggtct tcaagtcgaa ttcgatccgt tgggcctgat gccgcggaaa ggcagtaatg
     3601 gcctacttcg caaagaaatc gaccagccag gggtaatagg tcatcaaggc agcccggttc
     3661 aggacaaaga caaggtagct ccccatcccg atgcaggact ggatgatggt cagaatgtcc
     3721 tttcggggct ctttccttgc caggtacccc agggctccga cacaagctat aaaggctggg
     3781 atgtggaagg ccggcccttt ttcgatgcct gccttttgca tcgcctctaa ggtccggagg
     3841 atgcaatagg ccccccaggc cgccagtata taagtctccc cgaaaaagaa aacaatcctc
     3901 aggggatacc acaccagggg gagaacggcc ggcggcagct tttcggtctc gccgtagggc
     3961 gcctcctcgg gcaggtcttc accttggaaa aaccgcatga ctgtcatgac cggcttttga
     4021 atgcaccagg tgtaaaaaag ggcgaaagtg atcgccgcac cgctcatgga caaaaagaaa
     4081 agaatgcgca tgaaggacct cctcgggttt cactggaaaa atgccctgaa ggatactata
     4141 tctcaaaaag gggagactac ggccctccca ttctttagac ctcgtctttc gtggccaggt
     4201 cgtgcaggag tatcatcttt ctcaactgga cgttcgaggg agccctgttg ccgtcaaaaa
     4261 ggacttccag cggcaaccct tgggggaagc ctcccgttcc ccgggtacta tcttccgtaa
     4321 ctgaagatgc cttccgaatg atcaaggata attcctccct tcagtttccc tgggttcgct
     4381 gacacttgga ccccggcgga gcatcccaag tcatttcatg agatcaatag tgacattatg
     4441 taatctattt gtcaactatt gaaacaagga atctcctcca ttcagagggc gatccattcc
     4501 cggcccttcc ctggacccaa tcggccgcac ctccacccct tgcctgcaac gtatcggctt
     4561 cctctaaaaa caccctcagg tctacctgga gatcctttcc ggccgcgagc ataaatgccg
     4621 cctccccgct ttcttcccgg gagagaaagc cgacgacgca accggtttcc ttttcaatct
     4681 ttctttttac tcccgaagaa gccaactccg gtgggaaatt ctctatggcg aagaagtaca
     4741 acttccccgg ggtctcgccg atatcctccc aggggatctc gatatatttc ctgaccttgt
     4801 ccagttttct tgttgcttcc ctccgctcct ccctgagcct ggcgaccacc gaggggaggt
     4861 cttcctcttc gcagccgatc tcccttgaaa gcaccctcgt tatcctgctg tgcctctgta
     4921 atgtctcctg gcgatcaacg gtgaacttga cctcccattg cccattgcca cccttgaagc
     4981 cggtgacgat caaccccccg acctccgatg tggatccgac gtggctcccc gagcaagcca
     5041 tgctgtcgaa ttcaccgatg gtgacaatgg tcacttctct ttcgtcgatc ctgtcccact
     5101 tgatctttac tccttcgaaa ctatcgcctc tttccctcga gaccctttta acaccgaccg
     5161 ggaggtccgc cgcgataacc ctgttggcgg cctcctccgc cctgaacagg tcatcccaag
     5221 cgatatcccc gggataggtc atgaaaacca cgctctcctt ttcacctatg gagacttttt
     5281 gcacgtccag cccgggtagc tggttttcga gcgtcctgga aaggatgtgt tcggcagtgt
     5341 gcattctcga aaaacgtcgg ttcctttcaa aatcgactgt ggcgtcgacc gggcggccca
     5401 gggggatctc accctccttg accactatat ccaggacatc accttcattg ctggtgcggc
     5461 aatcgagaac cctggccttg aaactctcgc tttcgaggca accgctgtcg ccgggctggc
     5521 cccctccgga tgggtgaaaa actctacccg aaacccttat ttttgctttt tcccctttcc
     5581 gttccagaac ctcttctatc tgaatctttt cattcatttc attgaccaac ctccccattg
     5641 gaaaactccc aaaagatact tcgctggatg gtaaacttgt ttacacccgc ggaggatcac
     5701 ttaatgggcc aagaaaagtg tgtcagggtc attataacag ggacggtcca gggagtaggt
     5761 ttccggtgga gggcaaggca ggccgccctt tcgctgggac tatccgggtg gataaaaaac
     5821 cgcctcgacg gaggggtcga ggcggttttc cagggtcctc cagaatcggt ggatcaaatg
     5881 atcgagtgga tccaggtcgg accccccgga gccgtagtga gggaggtcat gtacctccac
     5941 tgtgacccgg accttggccg atccgacttc ctcatcctga gatccactta actgacacgg
     6001 ttaccctcac ttggcaccac ctactacctt aagctcgggc accttcgttt cgacttcagg
     6061 gtggacctgc ctccagagtt tgacggggag cccccagatc tttatgaacc cggccgcagc
     6121 ctcgtggtcg aaggcatctc cctcagaata ggtggccatg gattcgacat aaagtgactt
     6181 ttcggccttt atccccacca ccaccgacgc gcccttgaaa agtcgcaccc tgacagtgcc
     6241 ggacacgttc tcctggatgg aatcgaagaa ggcatcgaga gcctttttca gtggcgaaaa
     6301 ccagaaaccg gtgtaggtga gttccgcgta ttttagcgag aggtccgcct tggccgctat
     6361 gacgtccttc gtcagggtca tgctctccag cgcctggtgg gcctggagga ggcataccgt
     6421 agcggggcat tcataaacct ccctgctttt gaatcccacc agccggtcct cgatcatgtc
     6481 gatcctgccc accccatgcg ctccagcgat cctgttcagt tcagcgatca acgcaacacc
     6541 gtccataatg gtgttgttga tcgccaccgg aatacccttt ttaaaggtga tctcgatgta
     6601 ttcctgccca tccggagctt cccagggatc caccgtaagg gcgaaagcat cactgggagg
     6661 ctccgtccag ggatcctcaa ggggaccgca ttcaatggac ctgccccaca agttcccgtc
     6721 aatggagtaa gggctttccg ccgttacagg gacggggata ccgttttcct tggcataagc
     6781 gatctctgca tccctggaga aatgccattc cctgaccggc gctatgacct ttagttccgg
     6841 gtccagggca ttggaacaga cctctatcct gacctggtcc tgacccttcc cggtacaacc
     6901 atgggcgacg gcgacggccc cttccatccc ggccacccac acaagatact tcgcgatgat
     6961 cggcctggaa agagctgaat tcaggggata tttgccctgg tagagggcgt tggccttcag
     7021 tgccggccag accatggtct ccacaaactc ctgcctgaga tccatgacgt aggcgttgac
     7081 tgctcccacc ttgagcgcct tctccttgac ctcttccgta tcgaggatct gccccacatc
     7141 ggccgtcagg gtcaccacat cgtaaccctg gtcgctcagc cactttatcg ccaccgatgt
     7201 gtccaggccc ccgctgtaag caagaattac ctttcccttg tattccatcc tggttccctc
     7261 ctttaaataa caaaaaggct cgccccttct tacgaagggg cgagccttgc ccgcggtgcc
     7321 accctacttt tcggcctctt tagcctactc tcacggtttg aaaccgttta ccccctaatt
     7381 gcctttttta aggactttct tcgggagtcg gttcaggggc tctattcggt cggtctcggc
     7441 gaggaacgct ttcagcggtt tgcatcccct ctctgtcgac gagcacacga cctactcttc
     7501 cccgtcatca ccgcgtgatc cgcgaattcc gacgcagaca gaccaggtct tattcgtcca
     7561 gctcgtctta agcgagtaag ggtcataacc acaaaagcct cccagatatt ttcgataatt
     7621 ggcgaatata atacaccgct ctcaggaaat gtcaacttgg ataccttcga gaggcgatga
     7681 ataactttat tttggttcct ttactgaacg gcagcaagga ggctgctcca aagcaagttc
     7741 aacccgggtc cgttgatgcc gaagcggacc ccatcggctc aataatcctc gaacccggct
     7801 tcgcgaaggg ccgttatgga gggataatgg gtcatgtcca tgtggatggg cgttatcgac
     7861 acataccctt cgacaaccgc cccgatatca ctcccgtcct cgctgacgtc ctccggcttg
     7921 ccggatatcc agtaacagga ccggccttta gggtccttga gaggggtgac cttttcccta
     7981 tagacccgga cccctttcct cgtgatcctg tagccctgaa cttcactcac cgggacattg
     8041 ggtacattca cattcaaaag aatgttcttt ggcagggggt gctcccttac cctgccaagg
     8101 ataaagacca ctgcctcagc ggccgtactg taatgttcct cttcatcgtc aggcctgcag
     8161 tttagggata cagcgatgga tggcttgcca aggaacaatc cttccatggc cgccgaaacg
     8221 gtcccagagt aagtcaggtc gtcggcgagg ttggggccct tgttgatccc cgagagcacc
     8281 atctctgaat caggggctac agcctccagg ccaaggacca cgcagtcgga aggggtgccg
     8341 tcgcaggaaa aggcttccag gtgttcaggg taccctcctc cctggatcga cctcagcctg
     8401 aggggcttgt ggagcgttat cgcgtgaccc acgctgctac gctccctgtc cggggccacc
     8461 acgcagggtc ggtagccttg atcgtgaagc tttctgacca gggtaacgat ccccggggcg
     8521 aatgcaccat cgtcgttgga gacaagaatt ctcaagggac cctttcactc cttccggtca
     8581 tcagagtatc aagtggaaca gggtcattat catgggagtg atcaacatct ggaccacccc
     8641 gagggccaga aggaccatca ggatgaagat gccgtacctt tcaagccaga aaaaacccct
     8701 caaccatgac ggaggcagga atagggatag cagtttcgaa ccgtcaaggg gcggaatggg
     8761 gacaaggttg aaaacaccca gcccaagatt gatcaggatc atgaggacca cgaatcttcc
     8821 cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcccca tcctcgccgg
     8941 agtagggtcg ccgagaagat cggcggcgta gccgtgacag aattcatgaa aagttatagc
     9001 ccagaggacc gccggtaagc tcagaagcaa gtcccctagg gcgggaatgt tgcccaaatc
     9061 ttcacacctc ctaaacttta gcccgttccg gccccaaaag ggagaggtgg aaattatccc
     9121 tgacccattc tatttcatct tcgcgggttt ccacttcccc atccagccgg gccatctgca
     9181 gccgggaaag gatactcccg atgagagggc cttcctcgta tcccatctcc agcagttcac
     9241 ggccaaccag catgggctcg accttttgga gacgggtcag gtaaaggagt atcctcctcc
     9301 tcacccgcca gcgttccgtg gcggcggccc agtagaaagc cgttatgggg ttaaactcct
     9361 tcaggaacat aaaaatgttc gaaggtttct tttgtcccct cccgccgagt ctgtgttcag
     9421 ccggtccaag ttcaaagagc cccttcatga tcaagtctct ttcggcgggc gaaaggttga
     9481 gcctgtctag gacttgcaag gctaccgtat cggttgagtc ggtgaggagc gccatcaaaa
     9541 agaaaagcca ctgcctgttg cccatgtcgg gaatatccct ggtaagcctc ttcttgaaaa
     9601 cctcaaggcg cctggttgcc ctgagagccc ccttatcaac gacgacgccg ggaaagaccg
     9661 ccccccagaa accgacggag ttaagccttt tgatctgcgc caggacgttc ttctcctgga
     9721 aaagaggctc tatctcgctc ctgaggcgca cacccgacag cagggtaagc aaacccccct
     9781 ggatgcagtt ttctaaaagc ctgagggtgt tgtcctcgat ccggagaccc agtctttgtt
     9841 ccaggcgtat cccccttatg acccttgtcg ggtcctcgac gaagctcagg ttgtggagga
     9901 ccctcaaaag tctgtccttg atatcccgcc tgccgccgaa ataatcgacc agggttcccc
     9961 aatcctcggc gtttatcgaa agggccatcg cgttaaccga gaaatcccga cggtaaaggt
    10021 cgtgctttat cgagtcgatg gatacctggg gttgtgcaac ggggtattca tagaactccc
    10081 ttctggctgt ggccacgtca accttccggc cgtcggggaa aatgacggtc cccgtccgga
    10141 atctctggtg gatcgccact cgcgccccat ttcttaccca cgattcgagg aaagccacgg
    10201 catccccttc gacaacaata tccaggtcct cgtttagtct cccaaggagc aggtctcgca
    10261 cgaaaccgcc gacaacgtag gctttcatcc ccatggaggt ggccctttct cccatccttt
    10321 tcaggaggga ggagaccggg tcattgagaa gggatgccat caggcccgaa atattctcaa
    10381 cccatggcat ctccggtgcc accgcccttg tctcggcggg cagggacctg gggaagaggg
    10441 ccctgaggat atcggtcctg gtgacaatcc ccatcaatcg tctgtccctt gtcaccatca
    10501 acctgccaat gtttcgggag accatgatcc tgtgcgcttc ggcaacggcg gccccaggac
    10561 cgatggagag aggatcctcc gtcatgtaat cttcaacctt ggccgcgcct aggccgtggg
    10621 tcgtagccct gtcgaggtcc tttctcgtaa tgatccccct gaccctgccc tcaagtgcaa
    10681 cgggaagagc ggaatgccca tacctgatca tcagtcggta agcgtcttcc actgattttt
    10741 ccggtgatat aaccatcacc gggctggtca tgacctcacc aacggtcacg aggggttgga
    10801 tggacctttc aagcctcacc tccaggtcag ccagcacctc ctggggagtg accccggtca
    10861 gggtcaccgc cgcggcctgg gaatgccccc ctcccccaag gggggaaagc aggccggcga
    10921 catcgaggac ctcctcccgt ccccgggcca cgacgtaaac tcttttttcc atctgaacgg
    10981 ccgccagggc cacatctgcg tcgaaataat ccctgagacg atggacgaag agcgagagtc
    11041 cctcgacata ggtcgagctg gtcatagacg ataggaccac cttggccccg ttgatgtacc
    11101 tctcacgggc gttttcgatc attttgtcct gtatctttct ctcggaactg tcaagggaga
    11161 gttcaatatg ggcggggatg gcggtcatat ccgcccccag gttcttcagg ttgaggatag
    11221 cccggaagtc cttttcggtt gtggctccga aggtcagcgc tccggtatcc tcgtatatgc
    11281 ccatggcgaa aagggtggct tcctggggcg atatggggat ccctttttca agcagtttcg
    11341 ctacgaggat ggtggtcgtg gcccccacgg gttcaatcag cgaaaaagag gcctcgatct
    11401 cttcctgcac tgccgcatgg tggtcgtaaa catgaacctc gagtccaggg cggccaagga
    11461 tacccgcgaa ggaagcgatc ctggcgggag agcgcgcatc cacgacgatc agcatcgtga
    11521 tctctttcat gtcgatctgg ctgggtgtca ggatctccca tcttcccggc gtcctcttga
    11581 ggaattccct gacatttctc cccgccgacc cggagaagca ggcccgtgct cctgggtaga
    11641 gcttcgctgc ggccaccatg ctggccaagg aatcgaaatc agcaccgatg tgggtggtga
    11701 tgaccttcaa cacttctcta ccttcatagc tcgtaacaaa acctccttgg ggaatcgccg
    11761 cccctcaggt tggctcgatc ggacaaagat accaacgccg ccgatctttt cagccagggc
    11821 cttgatcccc tccaggtcgc aggatcccac gaaagccgtg acgataagat caggaccgcc
    11881 tcggtcagtg cccgcgacct cgcactcctc ctgggaagcg atccccacct cgatgaccct
    11941 ttcgtccctg gacaactccc tgaacaggcg acaggatacc accggcgtga ccgaaacgaa
    12001 acagaccctt gcgagggagg aaagagagga aaaccgcgat cccaggtctc tggtcttttc
    12061 ttccagttca tccagggtgc cgtcattact aatgatcagg tcggccctgg ttttcttgct
    12121 ttccggttca agaagccagc gttcacgctt gtggagttct tccctgtccc aaccacggaa
    12181 ggcgttgcgg cgcactctat cttcctccga cgcttcgacg taaacggtga ggtcgaccca
    12241 ttccggtacc ccgttctcga acaaaagggg gatctcggcg acaacccagc cgtctaggtc
    12301 ctcgatccgc cttttcatct cctcccggac aagggggtga atgatcccgc acgcccagcg
    12361 gtactccttc gcatcgctga agatcacccg ggagacggcc ccatggtcca gggtccccgc
    12421 ggcggtgagg acactccgcc cccacctttc gacggccagc gtcaccaggt ctcctctcct
    12481 ccagagttca gccactatcg ggtccgcact gatcacccgg gccccctggg atccccaggc
    12541 ggatgaaacc gtcgatttac cagccccaac gtctcccgta agacctatca ccagcatagg
    12601 atgcttcctc cccgctcatc tccccggttt ctttgaattc gtcggggatc tcccagagga
    12661 acctggaaaa accgttcctc tggatggtcc cgaaaaggac cctgctaccc gcgccggtca
    12721 agtagagcct ctcctcggcc cttgtcatgc ccacgtagca aaggcgtctt tcctcttcaa
    12781 gatcgtccgg gtcatcgagg caccggtaat gggggaacag tgcttcctca aggcctacca
    12841 tgaaaaccac cgggaactcc agccctttag cggcgtggag gctcatcagg ctcaccgact
    12901 ccgcctcgtc gggcgcaagg tcctggtcgg tcaccagggc tatctccgcc agcatcctct
    12961 cgagcccttc cgcggaggaa gtgaccgaca gcagttcccg gatgttgtcc accctctccc
    13021 cccagccttc ggggtccatc ctggtcaatt ccccctggta accgatcccc gccaagatat
    13081 aatctattac agaggggata tccccgcttc gcctattgat ctcaaccatg tgggccccca
    13141 gttccatgac ccccttgccg gccttaccgg ggagttcggc cccgtttttc gacagttttg
    13201 cccataacgc gatcccctgc tctcccgaag cgtggatatg gtccttcaat ctttccaggg
    13261 atttttggcc gagacttcga gagggcatgt ttcctatcct ctccagcgcg gcctcatcga
    13321 aagggttgac ggcgagtctc atgaaggaga gggcgtcctt gacctctcgc ctctcgtaaa
    13381 aagccgtccc ccgtactatc ctgtagggga tatcgttgcg gagaaaaacc tcctcgtaaa
    13441 gccggctcat ggcgtttatc ctgtatagaa tggccatgtc cccgaacctg aaaccgagcc
    13501 cccttagcct cagtacctct tcagtgatga agttcgcctc gaccctctcg ttacccgcca
    13561 gcagaacgtg gatcttctcg ccgccgctcc tcgaagtcca gagctttttc ggttttctgc
    13621 ggaagttgtt cctgatgacg ccgttggccc cgccgagaat attcggcgtg gaacggtagt
    13681 tttgctccag gacgaaaacc ctggcgtcgg ggaagtcctt ttcgaaatta aggatcatat
    13741 tcatatcggc ccctcgccac ccgtatatcg actggtccgg gtcgccgacg accatgatcc
    13801 tctcccgtcc cgaggagagg gcctttagga gcaggtactg gggacggttt acgtcctggt
    13861 attcatccac caggatccag tcgatgcgct ccctctccct ttcgcgcaca ttgctatcat
    13921 tgacgaggag gtgcaagggt aaaacaagca ggtcctcaaa atctacagca ccctgcctgg
    13981 ccagcccttt ctggtacttt gagtagatcg gcccgatcgg gaagtccagt tccccgatat
    14041 cgagggtcct ggggtctccc tcgctcttgg ccctgcaaag gcgttccacc acccagcccg
    14101 gttcgtactg tctcgggtca atatctagtt ctttcatgat gccctttacc agggacctcg
    14161 tgtcggcccg gtcaaagaca gtcgcggccc ttcgcccggt gatcctctca aggtcagggt
    14221 ggtttcttat gaggaagcga agtccccagg agtggaaggt cccgatctcc aacccggcaa
    14281 ggtctacccc caggagcctg tttatcctct cccgcatctc ccgggccgcc ttgtttgtaa
    14341 aggtcacggc catgatggaa cggggttcga ggccatgctc ggatacaagc caggcaaact
    14401 tgtgggccag gacgcgggtc ttgccgctcc ccgccccggc caggacgagc agcggccccg
    14461 aacaatagcg gacagcctcc gcctggctat cgttgaggga tgctatcagg cttgggtcat
    14521 ctttacgatt catggcctct ttgtcctgac ccagctttgg accaatgaaa cccaatcgga
    14581 catgccatct cccctgaggg ccgatacttc caacctttca gcccgagggt tcaattcctt
    14641 gacatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttt ttgagagatc
    14761 cttcctgatc ttttcggcaa ttttttcgtc ggcggccatg actgaccgct ggatcgctat
    14821 ccttcgtggc atattactct tccacctcca ccgactccag gtccatctcc atgccgctcg
    14881 cggtctcgac ttgatcgctc ccgcagcggg gacattgaaa tgataggccc tcccattcct
    14941 ccccgcaaaa agagcaccgg ctcatgaggg gtacttcaag gatctccagc gaggacccct
    15001 ccataatagt gccctcggtg accacggaaa aggaaaagat cattacctcg gggatgacct
    15061 gtctcatccg gccgatcctc aaggtgacct tcgtcacttt cttccatccc ttttccctgg
    15121 ccatctcctc caaggattcg accacagctc cgaccaagga caactcgtgc atttggaacc
    15181 tcttttcgat cccgttgaac aaggggtgac tggaatacta tccggagcga gcccaggcgt
    15241 ggctttacgc cgggcggaca ctggatcttc tttagtagta taacccttca gggatcctta
    15301 tctatcgggg tccctcaagg gagactcatt cccgacagat cccggatcat tgaaacccct
    15361 cgaggcggga gttttcccgt cgaccaggga tgacctcccg gtcactcaga acttccaccg
    15421 gcggaccata acccttcggt catggcacat cgatcgatga actgggcggg gcgaacggtc
    15481 aatcggacgc tatcaaaagg ccgcctcttg atcatttgcg gggataaccg gggggggagc
    15541 gaaagggtgg ggtcggctaa gaccccaccc gtcgggtccg ttctccttct tgggtcgacc
    15601 gatcactctt cgggaataag aagcccgaaa cctccatcct tcctcctgta gaccacgttc
    15661 actttcccat cgtcggcgtt tctgaacacg aagaaagtat gccccagcag gcccatctgc
    15721 atggtcgcct cctcgggggt catgggtcgg aagggaaact tcttggtctt cacgatccct
    15781 tcctttttct cctccggttc ggcgatctcg ttgacaaaac cctcgatctc gaaggacacc
    15841 tcggccgtct tcatcctggc tttttccttc aggaaggatt tgtgcttctt gatctggcgt
    15901 tcgatatttt tcaggcactt atcaaaggcc ttcctgacat cggaggcata gtcctcgccc
    15961 ctcatgatca cgccattcac gtcggcggtt atctccacta ctttcatgcc ccggttgaaa
    16021 ctgacctcaa cttgtgtatc aaggatcctg tcgaaaaatt tttccagctt ggccagtttt
    16081 ctctccatgt aatcccgaag ttcgtcctga aggtctacgt tacgcgtgac aaacctgaca
    16141 tccatcgtgt tccccccaat ccgcgatatc ttcagggacg acatagttca gattttacca
    16201 tcgaaggggc ggccaaacaa tgatcctacc tcttcatccc tatgacatcc taatctaatg
    16261 atagtatcga tccttctctc cttgccctat aatgacccaa aggcatttac acccctgatc
    16321 acgaaaggaa cggcgatggt agatgatgaa gacctcggtg agagcatcca ggatggagcc
    16381 gtcggcgacc ctggcggtcg tgaacaaggc taaagcactt aaatacgaag gaaaaccggt
    16441 catttccttc ggcgccggcg aacccgactt cgactccccc gcggcggcgg cgagttgcgc
    16501 cgagagagcc ataaaagagg gaaagacgca ctacacgcag tccaatggca tcctcgaact
    16561 ccgagaggcg gtcgcttcct actactcgga ccgcttcgga ttagattacc cggctgacca
    16621 ggtcatcatt ggaccggggg cgaaaccact gatatacggc tccctggctt ccctggtgga
    16681 ccccgaggac gaagtactgg tcttttcgcc ggcatgggtg agttacgttg aacagatccg
    16741 gcttttggat gggagagcga gcatcgtcga taccgtcacg acagggaatg taccccttgc
    16801 ctccaaggtc gctgaagcgg taaccccccg gacggtgggt atgctcataa atacgcctaa
    16861 caacccgact ggagcggtat acggcgagga aaccctgagg gagatcgccg aaatagccct
    16921 ggagcacgac ctttggatca tctacgatga gatctacgaa aggctcgtct acggaaattc
    16981 tactcatctc aacatcgcaa cgttggtccc ggaggtcgcc gatcgcacaa tccagatcaa
    17041 cggggtgagc aaggccttcg ccatgaccgg ctggaggata ggctacgccc tcgccccaa
//
LOCUS       DAOSRG010000008        14188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4360_length_14188_cov_9.206892, whole genome shotgun sequence.
ACCESSION   DAOSRG010000008 DAOSRG010000000
VERSION     DAOSRG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_14188_cov_9.206892"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLM30_00720"
     CDS             complement(<1..925)
                     /locus_tag="PLM30_00720"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR53720.1"
                     /translation="MRTEILAVDREKPGEGPIRRAAGIIRGGGLVVFPTETVYGLGAD
                     ALSPEAVRKIFQAKGRPADNPLIVHVEDKKAAALVGQLDERSRRLMDLFWPGPLTLVV
                     RAAGGIPKEVTGGLDTVAVRMPAHPVAIALIRSSGVPIAAPSANRSGRPSPTTAGAVL
                     EDLKGRVELILDAGPTAVGVESTVLDVTGDHPVLLRPGGVSLEDLDEAVGPVLLPTGS
                     EGEGRSPGTRYRHYAPSLPVILVEAGKARAEGSHLPGQGARVAYVGMSPPPAGVMAAA
                     CFASVEEYAKGLFSALRELERSGAEVILAELP"
     gene            complement(922..2043)
                     /gene="tgt"
                     /locus_tag="PLM30_00725"
     CDS             complement(922..2043)
                     /gene="tgt"
                     /locus_tag="PLM30_00725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR53721.1"
                     /translation="MFQFRLGSKCRETRARVGEIRTGRGSVETPVFMPVGTKATVKAM
                     APFELQEMGAGIILANTYHLYLRPGHEVIREAGGLHRFMGWPGLILTDSGGFQVFSLS
                     TLRDVSDEGVTFRSHLDGSAHLMTPELSVEVQEALGSDIAMCFDECAPYPCTVEGSKE
                     AVRRTLLWARRCLDAHRSPGQALFGIVQGSIFEDQRIDCARELVSMGFPGYAVGGLSV
                     GEPHDEMYRVLDAVVPELPEDKPRYLMGVGYPPNLVEGVARGIDLFDCVLPTRNGRNG
                     TLLTRRGRMNIKNLEYERDFRPIDPECGCYACRNFTRAYIRHLFRSGEILASRLCTWH
                     NLHYLLELMGNIREAIWSGTFADLRKRFHEDYQDGSDGA"
     gene            complement(2043..2828)
                     /locus_tag="PLM30_00730"
     CDS             complement(2043..2828)
                     /locus_tag="PLM30_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR53722.1"
                     /translation="MPRFVDSHCHLAMGEFAPDVPEVLARAAGAEVERILVVGSDEEG
                     SADALELVRSKGEGRLFAAVGIHPHESSSALSGIPGTVKRMARFPEVTAVGETGLDFF
                     YDHSPRDVQVRVFSEHVALAREVKKPLVVHVRDAYPEALEVLQKERADECGGVIHCFS
                     GSLGDAVAAIDLGFFISFAGPLTFPRNAALRETAAALPLERLLCETDAPYLSPQPRRG
                     RRNEPSHVPFVYRALADARGIPLEECSRALWSNASRLFRWGAE"
     gene            complement(2901..4832)
                     /gene="metG"
                     /locus_tag="PLM30_00735"
     CDS             complement(2901..4832)
                     /gene="metG"
                     /locus_tag="PLM30_00735"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR53723.1"
                     /translation="MPGKNFYITTPIYYVNDVPHIGHAYTTIAADVMARYKRMCGFDV
                     MFATGTDEHGQKIQQAADRKGMTAQELVDSVVVNFRNLWEVLGITNTDFIRTTEPRHV
                     RVVQYIFQRLMDQGDIYKGSYEGWYCVPCETYFPESQVGDGKLCPDCGRPLQNMTEES
                     YFFRMSRYEKPLLDHYEKNRDAILPHSRYNEVLSFIKGGLRDQSISRTSVTWGIPLPG
                     DERHVIYVWFDALINYLTVCGYPEKEDLVGKFWPTVNHIMAKDIIRFHCIVWPAMLLA
                     LGLNPPATVFSHGWWTVEGDKMSKSRGNVVDPFEMADLYGVDAFRYFLMREIPFGLDG
                     DFSERALVGRVNSDLANDQGNLLNRTLQMIENFTGGAVPSGQGIPDELDGMIRDMALA
                     TLGSYRAKMDVYAFDEALKDVWTLIKRANKYIDETMPWKLGKEGNLERLGPVLFTLCE
                     VLRFTAAMTSPFMPGTARKIFDQTGIPGDPAELLLDELEWGGAAERRISKKDVLFPRI
                     DLKVWEKQKAERDAGKTAGPDPGDHEPEVTIEDFRKIELRVAQVLKVEPVPKSDKLYR
                     MDLDLGYERRTIVSGIREFRSPEELEGRKIIVICNLKPASLRGVVSNGMLLAAETADG
                     KSLALLTVDSDIALGSRVH"
     gene            complement(4833..5669)
                     /gene="rsmI"
                     /locus_tag="PLM30_00740"
     CDS             complement(4833..5669)
                     /gene="rsmI"
                     /locus_tag="PLM30_00740"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOR53724.1"
                     /translation="MPLLVVPTPIGNMEDITLRALRALREADVIACEDTRRTLKILSR
                     YGIRRPLLSYHAFNEKERAQDLAERLSRGETVALVSDAGTPGISDPGYELIRHSLEAG
                     YEVDVLPGATAVVPALLLSGFPPHPFLFEGFLPKRKGERRRRLEELRAFGGTIVFYLS
                     PHGPGKVLGDILEVLGNRPAVILREISKLHQERIPGDIASLLASLDEVPLKGELVLVT
                     GPAAVTRDISDEDFEEVLLGMLEEGLPEKEVVRRLVEEYGISKNKAKRLLLGKKCEKE
                     AL"
     gene            complement(5671..6396)
                     /locus_tag="PLM30_00745"
     CDS             complement(5671..6396)
                     /locus_tag="PLM30_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOR53725.1"
                     /translation="MRTTRDAILYGILMIEQPEEGPRVSVDSVLLASFVKARGRERVI
                     ELGSAHGAVSLLLAKRFPHVTVEGLEIQPGLVTLAEKNAAGNGLQERVTFREGDLRRV
                     REFYPAQSFGIVVVNPPYGEADHSRPSPFESEAAAKHGTLCTLEDVVKASRYLLANKG
                     RLFMVFRAGRVAELLSLLSRERIEPKRVRPVYPAPGRDATVVLVQAVRSAGRGLTLEP
                     PLFIQDEKGEYTGDLLAAYRIGG"
     gene            complement(6396..9809)
                     /gene="smc"
                     /locus_tag="PLM30_00750"
     CDS             complement(6396..9809)
                     /gene="smc"
                     /locus_tag="PLM30_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOR53726.1"
                     /translation="MSIGRIHLKGFKSFGGSHEIALCENFTAVVGPNGCGKSNILDAL
                     RWVLGDGNPSRLRILKQGDLLFQGSISLPPATSSEVMIHLKGEEKVSTLRRRFTNEEG
                     GTLLFEGRRIRLVDLDEIKRQWRLEGDRFAFISQGEVSEVIQQKPLQRRMHLESLFGI
                     DLYRKQREEAAQKLDAATLELQRIMTLQSELRTRKEVIADPVLRAREAKAVIDDLERE
                     KSRLYWVKRARSERVLESIGVELSDLGAQSRAASFWASGWGKALARLEGTLDAAAEDR
                     GAMAARLAGLEGDLTGVRKKLYALSTSLAGSLERRRGLQDDLAREGEGLSTVRARLED
                     LQKEADLAGEEEKEASRSFSEAKNRWEQYRGQVKQRLDAIRSLREERADGEAALGTAR
                     SRARALGLSYLEALGEISGAGERVTAACRELAGLKKDLEDKKRAEEEARGAQRDAYAA
                     FQHSAAAIQKVSREISALEGQAEALRETAFTRLYPPAVQHLVAASRLGRIDASPRPLA
                     DVITCPERLVTALESFLGARQYLLLVKDIDEAGRCIDHLKARSAGRATFLPLESARPR
                     TPTRGIAGDRKGVVGWARDLLGMESDWERCVLHVLGDLLIVEDFDVARDLAREGYRGP
                     AVTLEGDVFQPGGTISGGRAPKGPGAMEIRREIQEKEKAAGDLKAEKARLEGSMAVLE
                     RKEENASGLLRLLSDERRALEEKIRLAEAAEGEAGRVLSRAERRKEEVGSALSECGRD
                     HLEIMTRLRGLGGDAPEDPDPSREVELVRALEESRSRAEVAAERSGNAGRLLSMTREE
                     LRNSSSRWERTRRELEGLDHAASTVLKDLSAGGKAYHASWVLLQEALSLLRALEVDHS
                     RALADLELARRRSTSASSRMDGLDLMIRQARQRLESTKAEAAESIDMWEERFPYPGGD
                     GIDTREYDKIRRNVREMERDLREIGDVDLGVLSEDLSLTQRLEFLEDQVRDVSTGIDE
                     LRRLIDETDRQAGALFLESLRSIDRKFCGLFQRLFGGGEAHLRLSDGDNPWEAGVEVI
                     ARPPGKRPQHLAQLSGGEQSLSAISLLFAAMEVAGAPIAVLDEVDASLDEVNLRRFSE
                     LAREYSRSMQLICMTHRRATMERADLLYGITMPEPGLSQVVGVRVEDWE"
     gene            complement(9784..>10167)
                     /locus_tag="PLM30_00755"
     CDS             complement(9784..>10167)
                     /locus_tag="PLM30_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HOR53727.1"
                     /translation="LTRKRARTDIRSSFTRGCPFCGGTGVVTREESVALSVKRFIRKV
                     ILSGRPEALLLELHPAVASFVAQNFLHSWEEEFRARLFLRAIHDSPWDKYRLEAQGTL
                     EQVEHRISLLENRMEGGIVHRTDPS"
     assembly_gap    10168..10267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10268..11435)
                     /locus_tag="PLM30_00760"
     CDS             complement(<10268..11435)
                     /locus_tag="PLM30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOR53728.1"
                     /translation="MISERKIFANVIDPEETRVAVVEGGRLNEIFVERMWDRQRSGDI
                     FKARVDSVLPGMNAAFLNLGEGRNAFLYLEDARGSVVKQNTDLLVQVTKTARKGKGAR
                     VTTRLSLPGRFLVLVPGGGETGVSRRIPEERREPLRSLAREIKPDGFGVIIRTAAEGV
                     DREALETDLENLVQQWKGIVSTAREQDAPCLIYQDFGLVGRILRDEPFGAVDEIIVDS
                     EEDYNHILSFLERFPPSWAPPEVSLHRGNIPLFEFWGIEKDLESALDRKVWLESGAYL
                     VIDQTEALTVIDVNTGKYTGGGDPRSTILRTNLESAREIARQLRLRALGGIVVIDFID
                     MEDEEDQGELLGTLRDLFRNDRARARVFGVTGLGLVELTRKRARTDIRSSFTRGC"
     gene            complement(11432..12052)
                     /locus_tag="PLM30_00765"
     CDS             complement(11432..12052)
                     /locus_tag="PLM30_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOR53729.1"
                     /translation="MARVRMIFSKRGRVCFVPHVEMPALFCRALRRAGAKVQLTEGMS
                     PRPKISLGPALPVGVPALGEPLEVWVENWEERLGEAVNGYLPGGVAVRRYGVVEGRPR
                     LNEECAAALYGVFFRDPRTFERARAMVSDGWVPVEGTLGISVSEGWVDVAVASPGQVG
                     GGTIVKSLVGEGLVRSWSELLVTRLAVGGWSWGEVTPLACEGSERG"
     gene            complement(12039..13838)
                     /locus_tag="PLM30_00770"
     CDS             complement(12039..13838)
                     /locus_tag="PLM30_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOR53730.1"
                     /translation="MYRAFDELEDPLWEMLSSVKRPSRYTGGEWGADGGRSMGAERVS
                     LCLAFPDVYEVGMSYLGFQVLYDLASKTPGVRVERVYCPWPDAESFIREKGMVLRSLE
                     TGKPLYSFDVVGFTLQYELAATNILTMLDLGGIPLRASERGEGSPLVVAGGPGAFAPE
                     PLAPFFDAFCVGDGELLLPDLLSLFPGKGRPRKELLEELSKVEGFYVPALNEGGAVRG
                     RVLMDLDRGFIPSGMIVPSSEIVHDRAAVEVFRGCTRGCRFCQAGMISRPHRERSPEV
                     VAEAAMGLLERTGYDEVGLVSLATCDYSGIEKVVDLLGPELRERNFRLSLPSLRMDAF
                     SVGLATRLEELGRGGLTFAPEAGTQRLRDVINKGITEKDMKDTFREVFSRGWDRVKLY
                     FMMGLPTETMEDVEAIMEVSLLARETGRKLGKRPRIGVSVAGFVPKPHTPFQWEAQAT
                     VEDLAMKGGTLKRMAKKAGITLNYHEPAQTFLEGVLARGDRKIADAIEKAWKDGARFD
                     GWTECFDLGRWLKAFEASGIDPVGYTGRTRGRDEAFPWEVVDAGVSRSFLWGERLKAL
                     AGELTNDCRGGACNLCGWQGRGCPVLETTDGQG"
     gene            complement(13859..>14188)
                     /locus_tag="PLM30_00775"
     CDS             complement(13859..>14188)
                     /locus_tag="PLM30_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOR53731.1"
                     /translation="HTDFVFSVFSEEFGFAGASLVLALFAILLWRMLSTSARSGDPRA
                     RILVEAVSAWIWFQLFENIAMAVGIAPVTGLALPFVSYGGSAMVSLGAALGLVQSIHV
                     SSRPQYE"
BASE COUNT         2370 a         4831 c         4253 g         2634 t
ORIGIN      
        1 cggggagttc ggccagtatc acctcggcgc cggagcgttc cagttccctc agggcggaaa
       61 aaagcccctt cgcgtactcc tcgacggaag cgaagcacgc cgccgccatc acgccggccg
      121 ggggagggga catacccacg taggccaccc gggctccctg tccgggcagg tgagaccctt
      181 ccgcccgggc cttcccggcc tcgaccagga tgaccggtag ggagggggcg tagtgccggt
      241 accttgtccc cggtgaccgt ccctccccct cactcccggt cgggaggagg accggcccca
      301 ccgcctcgtc aaggtcctcc agggacacac cgccgggcct aagcagcacc gggtggtcac
      361 ccgtcacgtc gagcacggtg gactctacgc ccacggcggt ggggcccgcg tcaagtatca
      421 attcgacccg acccttcagg tcctccagga cggccccggc ggtggtcggg ctgggccttc
      481 cgctcctgtt ggcgctgggg gcggctatgg ggacaccgga actccttatc agtgcgatgg
      541 ccacggggtg ggcgggcatc ctgacggcca ccgtgtcgag ccctccggtt acctctttcg
      601 gtatcccccc cgccgccctg acgacaaggg taaggggccc cggccagaag aggtccatca
      661 gcctgcggga cctttcgtcc agttgaccta ccagggccgc cgctttcttg tcctcgacgt
      721 ggactatcaa cggattgtcg gcaggccttc ccttggcctg gaatatcttc cggacggctt
      781 cgggggagag ggcgtcggcg ccaaggccgt agacggtctc cgtcgggaag accaccaatc
      841 ctcctccccg gatgatccct gcagccctcc tgatcggccc ctccccgggc ttttccctgt
      901 cgacggcgag gatctccgtc ctcaagcccc gtcactcccg tcctggtagt cttcgtggaa
      961 ccttttcctg agatcggcaa aggtgcccga ccatatcgcc tccctgatgt ttcccatcag
     1021 ttccaggagg tagtggaggt tgtgccacgt gcaaagcctc gaggccagga tctcccccga
     1081 acggaaaagg tggcggatgt aggcccgggt gaagttacgg caggcgtaac agccgcattc
     1141 ggggtcgatg ggcctgaaat ccctctcgta ttcaaggttt ttgatgttca tcctccctcg
     1201 gcgcgtcagc agcgtcccgt tcctgccgtt ccttgtcggg aggacgcagt cgaagaggtc
     1261 gatacccctg gcaaccccct cgacgaggtt cggcgggtaa cccaccccca tcaggtaccg
     1321 gggcttgtcc tcggggagtt cggggaccac ggcgtcgagc acccggtaca tctcgtcgtg
     1381 gggttccccc acggagagcc ctcccacggc ataccccggg aaacccatac tcaccagttc
     1441 gcgggcgcag tcaatacgct ggtcctcgaa gatggagccc tggacgatcc cgaaaagggc
     1501 ctggcccggc gaccggtggg catccaaaca cctccgggcc cagagaagcg tccttcggac
     1561 ggcctctttg gacccctcga cggtgcacgg gtagggggcg cactcgtcga agcacatggc
     1621 gatatcgctg cccagagcct cctggacttc cacggagagt tccggcgtca tcaggtgcgc
     1681 ggacccatcg aggtgggacc tgaaggtcac gccctcgtcg gagacatccc tcagcgtcga
     1741 gagggagaaa acctggaacc cgccgctgtc ggtgaggatc agcccgggcc accccatgaa
     1801 gcggtgaagg ccgccggcct cccggatgac ctcgtgcccc gggcggagat aaaggtgata
     1861 ggtgttggcc agtattatcc ccgcacccat ctcctggagt tcgaaggggg ccatggcctt
     1921 taccgtcgcc ttcgtcccga cgggcatgaa gaccggcgtc tcgacggagc ctcgtccggt
     1981 ccttatctcg cccaccctgg cacgggtttc gcggcatttc gatccgagcc ggaattggaa
     2041 catcactccg ccccccagcg gaagagccgc gaagcgttac tccacagcgc ccgggagcac
     2101 tcttcgaggg gaatgcccct ggcatcggcc agggcacggt agacgaaggg gacgtgggag
     2161 ggttcgttgc gccgtcctct ccggggttgg ggagacaagt agggggcgtc ggtctcgcaa
     2221 agcagcctct ccagcggaag ggcggccgcc gtctccctca gggcggcgtt cctggggaag
     2281 gtgaggggcc cggcgaagga gatgaaaaac cccaggtcga tggcggccac ggcatcaccc
     2341 aggctcccgg aaaagcagtg gatgacgccg ccgcactcgt cggccctctc cttctgcaat
     2401 acctcaaggg cttcggggta agcgtccctc acgtggacga cgagaggctt ttttacctcc
     2461 ctcgccaggg cgacatgctc tgaaaagacc cggacctgga cgtcccttgg ggagtggtca
     2521 tagaagaaat ccagccccgt ctcccccacg gccgtcactt cggggaaccg ggccatcctc
     2581 ttgacggtgc cgggtatgcc cgaaagggct gatgaggatt catggggatg gatgccgacg
     2641 gcggcgaaga gccgaccctc acccttgctt ctcaccagtt ccagggcatc ggcgcttcct
     2701 tcctcgtcgg aaccgacgac cagtatcctt tccacttcag ccccggcagc cctcgcaagg
     2761 acttccggaa cgtccggggc gaactccccc atcgccaggt ggcaatggga atcgacaaat
     2821 ctcggcaaga gcgcacctcc tggtaataga aaaggggccg ggaacacccg gcccctcaag
     2881 agcgggtgcc ccaagcgtcg tcaatggacc cgcgagccaa gggctatatc gctgtcgacg
     2941 gtgagtaggg ccaggctctt cccgtcggcc gtctcggccg ccaggagcat gccgttgctg
     3001 accacccccc ggagcgaggc cggtttcagg ttgcagatca cgatgatctt ccggccttcc
     3061 agttcctcgg gcgaacggaa ttccctgatc cccgacacga tggtccttct ctcgtaaccc
     3121 aggtcgaggt ccatcctgta aagcttgtcc gacttcggga cgggttccac cttcaggacc
     3181 tgggccaccc ggagctcgat cttccggaag tcttcgatgg tgacctccgg ctcgtggtcc
     3241 cccgggtcgg ggccggccgt cttcccggcg tctctctcgg ccttttgctt ctcccacacc
     3301 ttgaggtcga tcctggggaa gaggacgtcc ttcttgctga tccgcctctc cgccgcccct
     3361 ccccactcca gttcgtccag caggagttcg gcggggtcgc cggggatgcc ggtctggtcg
     3421 aagatcttcc gggccgtccc gggcatgaag ggcgaggtca tggccgcggt gaaacgaagg
     3481 acctcgcaca gggtgaagag aacagggccg agcctctcga ggtttccttc cttgccaagc
     3541 ttccagggca tcgtctcatc gatatacttg ttggcccttt tgatcagggt ccagacgtcc
     3601 ttcagagcct cgtcgaaagc gtaaacgtcc atcttcgccc ggtaggaccc aagggttgcc
     3661 agggccatat cccggatcat cccgtcgagt tcatcgggaa tcccctggcc ggagggcacc
     3721 gccccgccgg tgaagttttc gatcatctgg agggtcctgt tgaggaggtt cccctggtcg
     3781 ttggccaggt cggagttgac gcggcccacg agggccctct cggagaagtc gccgtcgagc
     3841 ccgaagggta tctccctcat gaggaagtag cggaaagcgt ccactccgta aagatcggcc
     3901 atttcgaagg gatccaccac gtttcccctg gatttggaca tcttgtcccc ctccacggtc
     3961 caccacccgt gggagaagac cgtcgccggc gggttgaggc ccagggcaag gagcatggcg
     4021 ggccagacga tgcagtggaa ccggattata tccttggcca ttatgtggtt gacggtgggc
     4081 cagaacttgc ccacgagatc ctccttctcc gggtaaccgc agacggtcag gtaatttatc
     4141 aaggcgtcga accagacgta tatcacgtgg cgctcgtcgc cggggagggg tatcccccac
     4201 gtgacggagg tcctggagat ggactggtcc ctcaggccgc cctttatgaa actgagaacc
     4261 tcgttgtacc gggaatgggg cagtatggcg tccctgttct tctcgtaatg gtcgaggagg
     4321 ggcttttcat acctggacat gcggaagaag tagctttcct cggtcatgtt ctgcaggggc
     4381 ctgccgcagt ccgggcatag cttcccgtcg cccacctggc tctcggggaa gtaggtctcg
     4441 caggggacgc agtaccaccc ctcgtaagat cccttgtaga tatcaccctg gtccatcagc
     4501 ctctggaata tgtactggac cacgcggacg tgccttggtt cggtggtccg gatgaagtcg
     4561 gtgttggtga tccccagcac ttcccagagg ttcctgaagt tgaccaccac ggagtcgacg
     4621 agttcctggg ccgtcatgcc cttccggtcg gccgcctgct ggatcttctg gccgtgctca
     4681 tcggtgcccg tggcgaacat gacgtcgaaa ccgcacatcc tcttgtacct ggccatcacg
     4741 tcggcggcaa tggtcgtata ggcgtggcct atatggggga catcattcac atagtagatc
     4801 ggcgtggtga tataaaaatt ttttcccggc attcaaaggg cctccttttc acacttcttc
     4861 cccagcagca aacgtttagc cttgtttttc gaaataccat actcttctac aagcctcctg
     4921 acaacctcct tttcgggaag gccctcttcg agcatcccca ggaggacctc ttcaaagtcc
     4981 tcgtcggaga tatcccgcgt caccgcggcc ggacccgtca cgaggaccag ttcccccttg
     5041 aggggaacct cgtccaggga ggcgagcagc gaggcgatgt cgccggggat cctctcctgg
     5101 tgcagttttg agatctccct taggatcacg gcgggccggt tccccaggac ctcgaggata
     5161 tccccgagca ccttgcccgg accatggggt gagaggtaaa agacaatggt cccgccgaag
     5221 gcccgtaact cctcgagcct tcgtctccgc tctcccttcc tttttgggag gaaaccctcg
     5281 aaaagaaaag ggtggggagg aaaacctgaa agaaggaggg ccggcacgac ggcggtcgcg
     5341 ccggggagga cgtcgacctc gtaaccggcc tcgagggagt gacggatcaa ttcgtagccc
     5401 gggtcggaga tgcccggggt gccggcatcg gagacgaggg ccacggtctc ccccctggaa
     5461 agcctttcgg cgaggtcttg ggcccgttcc ttctcgttga aggcgtggta agaaagaagg
     5521 ggtctcctta ttccataccg ggagaggatc ttcaaggtcc tcctggtatc ctcgcaggcg
     5581 atgacatcgg cctccctcaa ggccctcaag gccctgaggg ttatatcctc catgttgccg
     5641 atcggcgtcg gcaccaccag cagtggcatc tcaccctcct atcctgtagg cggcgaggag
     5701 gtcgccagtg tactccccct tttcatcctg gatgaaaagg ggtggttcca gggtcaaacc
     5761 cctgccggcc gacctcactg cctggacgag gaccaccgtc gcgtcccggc cgggggcggg
     5821 gtagaccggt ctgaccctct tgggttcgat cctctccctg gagagaaggc tcaaaagttc
     5881 ggcgaccctc ccggccctga agaccatgaa gagcctcccc ttgttggcca ggaggtacct
     5941 cgaggccttt accacgtcct ccagcgtgca gagagtcccg tgcttggcgg cggcttcgga
     6001 ctcaaagggg cttgggcggc tgtggtctgc ctcgccgtag ggggggttca ccaccacgat
     6061 gccgaaggat tgggcggggt agaactccct caccctcctc aagtcccctt ccctgaaggt
     6121 gaccctctcc tgcaggccgt tcccggcggc gttcttctcg gccagggtca ccagcccggg
     6181 ctggatctcg agcccctcga cggtgacgtg ggggaatcgt ttggccagga gaagcgacac
     6241 cgccccgtgg gcggacccca gttcgatgac cctctccctt ccccgggcct tcacgaagga
     6301 ggccagcaac acgctgtcca cgctcaccct ggggccctcc tcgggctgct ctatcatcag
     6361 tatcccgtat agtatggcat cgcgggtcgt ccgcatcact cccagtcctc caccctcaca
     6421 cccaccacct gcgaaagccc cggttcgggc atggtgatcc cgtacaggag gtcggccctc
     6481 tccatggtgg ccctgcggtg ggtcatgcag ataagctgca tcgaccggga gtattccctg
     6541 gccaattccg agaaccgcct gaggttgacc tcgtcgaggg aggcatcgac ctcgtcgagg
     6601 acggctatgg gcgcgccggc gacctccatg gcggcgaaaa gcagggatat ggccgacagg
     6661 gactgctcgc cccccgatag ttgcgccaag tgctggggcc tcttgcccgg cggccgtgcg
     6721 atgacctcta cgccggcctc ccaggggttg tcaccgtcgg agagcctcag gtgggcctca
     6781 ccgccgccga aaagcctctg gaaaaggccg cagaacttcc tgtcgatcga cctgagcgac
     6841 tccaggaaca acgcgccggc ctggcggtcg gtctcgtcga tgagccttcg cagttcatcg
     6901 atgcccgtcg agacgtcgcg gacctggtcc tccaggaatt ccagcctctg cgtcagggac
     6961 aggtcctccg acaggacacc caggtccacg tcccctatct cccgaaggtc cctctccatc
     7021 tccctgacat ttcttcttat cttgtcgtat tccctggtgt cgataccatc accgcccggg
     7081 taagggaacc gctcctccca catgtcgatc gattccgccg cctcggcttt ggtcgactcc
     7141 agacgctgcc tcgcctggcg tatcataaga tccaggccat ccatcctgga cgacgccgag
     7201 gtcgatctcc tccgggcgag ttccaggtcg gccagggccc tggaatggtc cacctccagg
     7261 gctcgcaaca gggaaagggc ctcctgtaaa agcacccagg acgcgtggta agccttgccg
     7321 cccgcagaaa ggtctttcaa aaccgttgag gccgcgtggt cgaggccctc cagctcccgt
     7381 cgggtcctct cccaccggga ggaggagttc cgcagttcct cccgggtcat ggaaagcagc
     7441 ctcccggcgt tccccgacct ttcggccgcc acctcggccc tggacctgct ctcctcgagg
     7501 gccctcacca gttccacctc ccgggagggg tcagggtctt caggggcgtc acccccgagg
     7561 cctctgagcc ttgtcatgat ctcaaggtga tcccttccgc attcagagag ggccgacccg
     7621 acctcctcct tcctgcgctc ggcccgggag agcaccctcc cggcctcccc ctcggccgct
     7681 tcggccagcc ggatcttctc ctcgagggcc cttctctcgt cggacaagag cctcagcagc
     7741 cccgaagcgt tttcctcttt cctttcgagc accgccatgg acccttccag gcgggccttt
     7801 tcggccttca ggtcacccgc ggctttttcc ttctcctgga tctccctcct gatctccatg
     7861 gcccccggcc ccttcggggc ccggccgccg ctgatggtgc cgcccggctg gaacacgtcc
     7921 ccctcgaggg tgacggccgg tccccggtag ccctccctgg ccaggtccct ggccacatcg
     7981 aagtcctcca cgatcaggag atcgccgagg acgtggagga cgcagcgctc ccagtcgctc
     8041 tccatcccga ggaggtccct ggcccacccc accacaccct tccggtcccc ggcgatgccc
     8101 cgcgtcggcg tcctgggcct ggcgctctcc agggggagga aggtggccct gccggcggac
     8161 cgggccttca ggtggtctat gcagcggccg gcctcgtcga tatccttaac gagcaggagg
     8221 tactgtctcg cgccgaggaa ggactccagg gccgtcacga ggcgctcggg acaggtgatg
     8281 acatcggcga gcggcctcgg ggaggcgtca atcctgccga gccttgaagc ggccaccagg
     8341 tgctggactg cgggggggta aaggcgggtg aaggctgtct cccggagggc ctccgcctgc
     8401 ccctccaggg ccgagatctc ccgggaaacc ttctggatcg ccgctgccga atgctggaaa
     8461 gcggcgtagg cgtccctctg ggcgccgcgg gcctcctcct cggcgcgctt cttatcttcc
     8521 aggtcctttt tcagccccgc gagttcccta caggcggcgg tcaccctctc accggcgccg
     8581 gatatctcgc ccagggcctc caggtacgaa agccccaggg ccctcgccct tgagcgggcc
     8641 gtcccgaggg ccgcctcccc gtcggccctc tcctcccgaa gggaccttat agcatccagc
     8701 cgctgcttga cctgtccgcg gtattgctcc caccggtttt tggcctcgct gaaggagcgg
     8761 gaggcctcct tttcctcctc cccggccaga tcggcctcct tctgaaggtc ctccagcctc
     8821 gctctcacgg ttgacaaccc ctccccctcc cgggcgaggt cgtcctgcag gcctctcctt
     8881 ctctccagcg atcccgccag ggacgtggaa agagcgtaga gctttttcct gacccccgtg
     8941 aggtcaccct caaggccggc gaggcgggcg gccatggcgc cgcgatcctc ggcggcagcg
     9001 tcgagggtgc cctccagacg ggccagtgcc ttgccccacc ccgaggccca aaaggaggcg
     9061 gcccgggact gggcgccgag gtccgacaat tcaacaccga tgctctcaag aaccctttcg
     9121 gacctggccc ttttgaccca gtagaggcgg ctcttctcac gttctaggtc atcgatgacc
     9181 gccttggcct ccctggccct caggacgggg tcggcgatga cctccttcct ggtccgcagt
     9241 tcggactgga gggtcatgat cctctgcagc tccagtgtgg cggcgtccag cttctgggcg
     9301 gcctcctctc tctgcttcct gtaaaggtct atcccgaaga gggactccag gtgcatcctt
     9361 cgctggaggg gcttttgctg gatgacctcg gagacctctc cctggcttat gaaggcgaat
     9421 cgatcgccct caagacgcca ctgccgcttt atctcgtcga ggtcgacaag cctgatcctc
     9481 ctgccctcga agagcagggt gccgccctcc tcgttggtga acctgcggcg cagggtgctc
     9541 accttttcct cccccttgag gtgtatcatg acctccgacg acgtggcggg gggaaggctg
     9601 atgctcccct ggaagagaag gtcaccctgc ttgagtatcc tcaggcgact ggggttgccg
     9661 tcgccgagga cccagcggag ggcgtcgagg atgttgctct tcccgcaccc gttgggccct
     9721 acgacggcgg tgaagttttc gcagagggct atctcgtggc tcccgccgaa gctcttgaaa
     9781 cccttaagat ggatccgtcc tatggacaat gccgccctcc atcctatttt ccagcaggga
     9841 gatcctgtgc tcgacctgtt ccagggtccc ctgggcctcg agcctgtatt tgtcccaggg
     9901 gctgtcatgg atggccctca ggaacaacct ggccctgaac tcctcctccc aggagtggag
     9961 gaagttctgg gcgacgaagg aggccacagc ggggtgcagt tcgaggagga gagcctcggg
    10021 acggcccgac aggatcacct tcctgatgaa acgcttcacc gacagggcga cgctctcctc
    10081 gcgtgtcacc acccccgtcc cgccgcagaa ggggcacccc ctcgtgaagg aggaccttat
    10141 gtccgtgcgg gccctcttcc tggtcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnggc accccctcgt gaaggaggac cttatgtccg tgcgggccct cttcctggtc
    10321 agctccacaa gcccgaggcc ggtgacgccg aagaccctcg ccctggcacg gtcgttcctg
    10381 aacaggtccc tcaacgttcc caggagttct ccctggtcct cctcgtcctc catgtcgatg
    10441 aagtcgatga ccacgatccc ccccagggcc ctcagcctca actgtcgcgc gatctcccgg
    10501 gccgactcca ggttcgtcct caggatggtg ctcctggggt cacccccacc ggtgtacttc
    10561 cctgtgttca cgtcgatcac cgtcagcgcc tcggtctggt ctatcacgag gtaggccccc
    10621 gattcgagcc aaaccttcct gtcgagggcc gattccaggt ccttctcgat gccccagaat
    10681 tcgaagaggg gtatgttgcc cctgtggagg ctcacttcgg gtggtgccca gctggggggg
    10741 aatcgctcga ggaaggagag gatgtggttg taatcctcct cgctgtcgac gatgatctcg
    10801 tccaccgcgc cgaagggttc gtccctgagg atccgcccca ccaggccaaa gtcctggtag
    10861 atgaggcagg gcgcgtcctg ttcccgggcg gtgctgacga tgcccttcca ttgctggacc
    10921 aggttttcca ggtccgtttc aagggcctcc cggtccacac cctcggcggc ggtcctgatg
    10981 atgaccccga aaccgtcggg tttgatctcc cgcgccaggg acctcaaagg ctccctgcgt
    11041 tcctcgggga tgcgcctcga gacacccgtc tcgccgcccc ccggcaccag gacgaggaac
    11101 ctcccgggga gggataacct cgtggtgacc ctggccccct tgcccttcct ggcggtcttg
    11161 gtcacctgga ccaggagatc ggtgttctgc ttcaccacgc tcccccgggc gtcctcgagg
    11221 tacaaaaagg cgttacggcc ctccccgagg ttgagaaagg ccgcgttcat tcccgggagg
    11281 acactgtcga ccctggcctt gaagatatca ccgctccgct ggcggtccca catccgttcc
    11341 acgaatatct cgttgaggcg gccgccctcg acgacggcga cccttgtctc ctccgggtcg
    11401 atcacgttgg cgaagatctt cctttcggag atcatccccg ctcactccct tcgcaggcca
    11461 ggggggtgac ctccccccac gaccagccgc ccacggccag cctcgtgacc aggagttcgc
    11521 tccaggagcg caccaaccct tcacccacga ggctttttac gatcgtcccg ccgccgacct
    11581 gtcccggcga cgccaccgcc acgtccaccc acccctccga gaccgaaatg ccgagggtcc
    11641 cttcgacggg gacccacccg tcggatacca tggccctggc cctttcaaag gtccgggggt
    11701 ctctgaaaaa taccccgtag agggcggcgg cgcactcctc gttgagccgg ggcctcccct
    11761 cgacgacacc gtaccggcgc acggcaacgc caccgggaag atacccgttg acggcttcgc
    11821 cgaggcgctc ttcccagttc tcgacccaca cttccagggg ttccccgagg gccgggacac
    11881 ccacaggaag ggcgggcccc aggcttatct tagggcgggg gctcataccc tcggtcagct
    11941 gcaccttcgc gccggccctc cgaagagcgc ggcagaagag cgccggcatc tccacatggg
    12001 gcacgaagca gacccgcccc ctcttggaaa agatcatcct aaccctggcc atcggtagtc
    12061 tccaggacgg ggcaacccct tccctgccac ccgcaaaggt tgcaggcgcc gcccctgcag
    12121 tcgttcgtca gttcccccgc cagagctttc agcctctccc cccagaggaa ggagcgactg
    12181 acgccggcgt cgacgacttc ccaggggaag gcctcgtcgc gccccctcgt cctgccggtg
    12241 tatcccaccg ggtctatccc cgaggcctca aaggccttca gccaccttcc cagatcgaag
    12301 cactcggtcc agccgtcaaa gcgggcaccg tccttccagg ctttctctat cgcgtcggcg
    12361 atctttcggt cgcccctggc caggacgccc tcgaggaagg tctgggccgg ttcgtggtag
    12421 ttcagcgtga tgcccgcctt ttttgccatt ctcttgaggg tgcccccctt catcgccagg
    12481 tcctccaccg tggcctgggc ttcccactgg aagggggtgt ggggtttcgg cacgaacccg
    12541 gccacggaga cccctatcct gggcctcttc cccagcttcc tccccgtttc ccgggcgagg
    12601 agggaaacct ccatgatggc ctcgacgtcc tccatcgtct ccgtgggcag gcccatcatg
    12661 aagtagagtt tcaccctgtc ccaaccccgg gagaagacct cccggaaggt atccttcatg
    12721 tccttttcgg tgatgccctt gttgatgacg tcccgaagcc tctgggtccc ggcttcgggg
    12781 gcgaaggtaa gcccccccct ccccagttcc tccaggcgcg tagcgagccc cacggagaaa
    12841 gcgtccatcc tcaggctcgg cagggagagc ctgaagttcc tttcacggag ctcagggccg
    12901 aggaggtcca ccactttttc tatcccgctg tagtcacagg tcgccagcga cacgaggccg
    12961 acctcgtcgt agccggtccg ttcgaggagg cccatggccg cttcggccac cacctcgggg
    13021 gacctctccc tgtggggcct tgagatcatg ccggcctggc agaagcggca acccctggtg
    13081 cacccccgga agacctcgac agccgcccta tcgtggacga tctcgctgga ggggacgatc
    13141 atccccgacg ggatgaaccc cctgtccagg tccatcagga ccctgcccct gacggccccg
    13201 ccctcgttaa gggccgggac gtagaaaccc tcgaccttcg acagttcttc caggagttct
    13261 tttcgagggc ggcccttccc cgggaagagg gagagcaagt ccggcagcag gagttcgccg
    13321 tcgcccacgc agaaggcatc gaaaaagggc gccagcggct ccggcgcgaa ggcaccgggc
    13381 ccgcccgcca cgaccagggg agaaccctcg cccctttccg aagccctaag ggggatgccg
    13441 cccaggtcga gcatggtcag gatgttcgtg gcggccagtt cgtactggag ggtgaagccg
    13501 accacgtcaa aggagtagag gggttttccg gtctcgaggg agcgcagcac catgcccttt
    13561 tcgcggatga aggattcggc gtcgggccag gggcagtaga ccctctcgac cctgaccccg
    13621 ggggtcttgg acgccaggtc gtagaggacc tggaatccca ggtagctcat gccgacctcg
    13681 tagacgtcag ggaaggcaag gcagagggag accctctccg cacccatgga gcggccgccg
    13741 tcggcccccc attccccgcc ggtataccgg gagggtctct taaccgagga gagcatctcc
    13801 cagagggggt cctccagttc atcaaaagcg cggtacataa aaaacctcca ccgaaagatc
    13861 actcatattg aggcctcgac gagacatgga tgctttggac cagcccgagg gcggccccca
    13921 gggagaccat ggcactcccc ccgtagctga cgaagggcag ggccaggccc gtgaccgggg
    13981 ctatccccac cgccatggcg atgttctcga aaagctggaa ccatatccat gccgagaccg
    14041 cttccacgag gatccttgcc ctggggtctc ccgacctggc cgacgtactg agcatcctcc
    14101 acagcagtat cgcgaagagg gcgaggacga gggaggcgcc ggcgaaaccg aactcctcgg
    14161 agaagacgct gaagacgaag tccgtgtg
//
LOCUS       DAOSRG010000009        14156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4373_length_14156_cov_9.279767, whole genome shotgun sequence.
ACCESSION   DAOSRG010000009 DAOSRG010000000
VERSION     DAOSRG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_14156_cov_9.279767"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLM30_00780"
     CDS             <1..602
                     /locus_tag="PLM30_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR53732.1"
                     /translation="IGKSAMSALLATFGLSMLLKNVCLNRFSPNFRILQHTLLGGKTF
                     NLAGIIIPLPQLVIGLVALALLYLIYLLVKKTRLGWAIQATAMDKEAAELMGINTERI
                     YLLVFGLGGACVGIAGGLLPSYLAVHPEVGGLFSLIAFVCVAMGGFGSIPGALLASLL
                     IGLVEAFAGFYIAPVFKYVAVFGLYLVVVMVRPRGLFGW"
     gene            596..1609
                     /locus_tag="PLM30_00785"
     CDS             596..1609
                     /locus_tag="PLM30_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR53733.1"
                     /translation="MVTAMTLTGKRTKRDLFWLGLLALFAVIPLTPWFTRSTFAQHVM
                     IQVLLYASMAQAWNILGGYCGQVSFGHSVFFGIGAYGTAMAVVKFGLIPWPGILAGAV
                     IAAVVAVAISYPCFKLKGHYFAIATFAIVEIFNRLFMVWYWVGGALGLDYGFTQPGWK
                     NFVWYQTKDCCFYGVLLLFILVFGIVRWIEKHRFGFYLRAVREGQDTAESLGVDSTRV
                     KLSAMALSAFLAALCGAFFAQYNYRVDPPMVMSLDMSMKFVLITILGGLGTFYGPLLG
                     AALLIPLQEYTRAYLGGIGGGVDLIIFGLLIIIVVVYQPKGIMGILDRVYVRFRRSRE
                     GGK"
     gene            1611..2369
                     /locus_tag="PLM30_00790"
     CDS             1611..2369
                     /locus_tag="PLM30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR53734.1"
                     /translation="MALLEVRNLTMKFGSLVACNDVSFDVEKGSVVGLIGPNGAGKTT
                     LFNCVSGFYKPTSGRMFFAGREVTNLPAHEVARRGAVRTFQVVRPLKEMTVMDNILVG
                     AFLRGADAGTAREVAGECLRLCRLEEMADNLAGGLPTGYKKRLEMARALATKPSLLML
                     DEAMAGLTTTEIRESIEIIRGLKKGGVTLLVVEHIMEAVMSIADKVVVLDSGVKIAEG
                     TPRQIVEDEKVITAYLGAKFAERMKRTGGVTRND"
     gene            2362..3078
                     /locus_tag="PLM30_00795"
     CDS             2362..3078
                     /locus_tag="PLM30_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR53735.1"
                     /translation="MTDPILRVQGFHSGYDGVPVIHGIDLEVAEGELVAIVGANGAGK
                     TTTMRTIAGLMHPFKGCVLFEGEDISRKKAHETNRMGISYVPEGRRLFPKLTIEENLE
                     LGAFTKTDREEIERNLEQVFDLFPKLFDRRSQIAETLSGGEQQMVAIARGLMSNPRLL
                     LLDELSLGLMPSLVEKVMEAVVSINRSGVTILLVEQMVQEALEIADRGYVLQTGRIVQ
                     QGTARELLDSPEVRKAYMGM"
     gene            3170..3754
                     /locus_tag="PLM30_00800"
     CDS             3170..3754
                     /locus_tag="PLM30_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOR53736.1"
                     /translation="MGGNAQIYIELEGITPKIWRRVVVPSSITLDRLSDVIQIVMGWS
                     GEHMHLFSIKGRQYMDLPEDEPEAHEEGLFRLGSLVSPKDGAFTYTYDLGDNWTHLVK
                     VEKMGLEEDEEWGNLFCVDGKGTCPPDDVGGVPGFLEFRQAMADRKGSQYEVFRDWYG
                     EEFDPDAFDQLEANITLLFFIRWSRERLLPWEED"
     gene            complement(3759..4334)
                     /locus_tag="PLM30_00805"
     CDS             complement(3759..4334)
                     /locus_tag="PLM30_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR53737.1"
                     /translation="MKTGEIILESLSPGRRRQIIECLDGIRGRADAGKVFLRPYRAGE
                     LGLVSWRHCVLYKKEYGFDETFEYYLLAGMAQLLHDLGGKGEVWVADCGGNVVGSIAI
                     VETSAAVGQLRWFLLEPAFRGLGLGRALMDTALEYSRDRGHGRVFLWTVSELHAARHL
                     YQVFGFRLTETKTHFLWGRDITEERWDLEFS"
     gene            4481..5176
                     /locus_tag="PLM30_00810"
     CDS             4481..5176
                     /locus_tag="PLM30_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53738.1"
                     /translation="MTTRTHNLPKDRGIFMKGFYKRFASLIFGLFLYSLGIAITMKAN
                     LGYAPWDVFHKGISNMTGITIGNASILIGLIICVTVALAGEKLGLGTILNMILIGFFL
                     DLILVVDKIPLMKGFLPGVAMMTFGLFVISIATYFYLGSGFGAGPRDGLMVVLERRTG
                     LPVGLCRAILESMAVFLGWLMGGPVGLGTAFAAFGIGFCIQITFTLFRFRATEVRHET
                     IRETLRDFRLSHR"
     gene            5273..6022
                     /locus_tag="PLM30_00815"
     CDS             5273..6022
                     /locus_tag="PLM30_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOR53739.1"
                     /translation="MPIRIVFHGELTGLLNNRGLSPEATRDLFRAAPVKDLIESQGVP
                     HTEVGRITSEGLERPFSHVPAWGETVEVHPHRPPVDVSLSSVLFPEPFEEVGFVADSN
                     VARLGGLLRLAGFNCLLDPRWDDARIAETAGANKRTLLTRDKELLKRNRVIRGRLIRA
                     ERPWDQLAEVIHFFGLSDKIDLFSRCPKCNVLLEGTEKSAVIDQLEPLTRMMYDHFTR
                     CPGCGQVYWSGSHHGRIREKLAAALEQASKD"
     gene            complement(6049..7413)
                     /locus_tag="PLM30_00820"
     CDS             complement(6049..7413)
                     /locus_tag="PLM30_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOR53740.1"
                     /translation="MDWEKFVDLSVKDAPRMLVTPPGPRSLEILKIQEELEGSAVSYP
                     RGMPMALARGRGATLEDVDGNVYIDCFSGAGVMALGHAHPFVLEAAHGQLDKVTHTLD
                     IPSETRMRLVKVLGRILPAELSRVFFGGPTGSDAVEQAIKLARFNTRRDGIIVFEGAY
                     HGMTGASLAATSDSGHKDGIGSLAPGFQFLPFPYRYRNPFGCPEEKVDEFAADNLERV
                     LDDTHSGYSKPAAVLLEAVQGEGGTIIPGPLFLQRVREITEARGILLICDEIQCGLGR
                     TGKMFAFEHSGIVPDIVTMSKALGGAGFPISAIAFREELNTLPPGKSIGTFRGNMVAF
                     AAGAAGLEWMIENRVPEYAAQLGEKAMARFREMEKGSRILGEARGIGLMIALEIVEDK
                     KTRKPAGGLTKQLRKLAHQRGVMIEVGGHHNNVARLLPPLVISEAHLMKAADILEGVL
                     KELE"
     gene            complement(7685..8839)
                     /locus_tag="PLM30_00825"
     CDS             complement(7685..8839)
                     /locus_tag="PLM30_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR53741.1"
                     /translation="MKRTVMLILALVLVFGAVGAASAADTIRIGLLAPLTGYAAADGL
                     AVLQSVQLAVDQVNAKGGVLGKQIELFYEDDAGEAKEAIGLARKLVQMDKVVAVVGGS
                     YSMPTRAVAPLFQEFEIPLVAGYAVHPDITAAGDFCFRNGFLGTVEGRGAGVAAVDML
                     KAKTVGMLTMDNDFGRQMAEGFKSYVVSKGAKVVYSQVYPLGEKDLMPYLTAVKEADP
                     DVILASGYYAEAAGIVKQAHEIKLRTQIIGEEGYDSPKFIELAGPEAANGTIIVTNLD
                     RDDERPVAQAYIKEYRERYGMEPDMTGASNYDAFLIIVDGIRRAGTTDPHKVKDAIAA
                     LKDFDGVTGVIKGFTEIGEVIKPVQVQIVKDGVFRRYGIVDDPEVIDPRK"
     gene            complement(8900..9655)
                     /locus_tag="PLM30_00830"
     CDS             complement(8900..9655)
                     /locus_tag="PLM30_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR53742.1"
                     /translation="MLEVENLTVAYGDILALQDVSFFLEAGEMVSIIGANGAGKTTLL
                     NTLMGLVKARSGRALYVEPSKDGGNGGRDILQLPAHERAALGIRIVPERARVFPNLTV
                     EQNLRMGVYGLSGSVDVERAFGEVYELFPVLKERRRQAGQTLSGGEQQQLAIARALVS
                     SPRILLVDEISMGLMPKLVDLVFSVLKRLNDEKGLTILLVEQNAVKALAIAHRGYILE
                     TGRIALEGKASDLSQNPKIREAYLGGDAAGGSV"
     gene            complement(9660..10418)
                     /locus_tag="PLM30_00835"
     CDS             complement(9660..10418)
                     /locus_tag="PLM30_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR53743.1"
                     /translation="MPGEDLILRVEKMSRHFGGLKAVDNVSFHVRRGEILGLIGPNGA
                     GKTTCFNVISGVYRPTSGQIFLDGRRIDGLPAHSIAARGVGRTFQIVRPFGGMTVLNN
                     VMVGLGMPRYGSFIKSSPLFNNSKNRGQAMTFLNEVGLEGFADSTASILSLGNLRRLE
                     LARALALGPKLLLLDESFSGLSQEEVGRMMGLVRKIQERGFTILLIEHNMRVAMSLCE
                     RLVVLDHGGKLAEGTPDEVRSDEKVIEAYLGKGE"
     gene            complement(10421..11293)
                     /locus_tag="PLM30_00840"
     CDS             complement(10421..11293)
                     /locus_tag="PLM30_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR53744.1"
                     /translation="MGGYFSTVAIMIGIYALVASGLNIIVGFAGQISLGHAAFFGIGA
                     YSSALLCTKAGLSFWWSLPLATLITGMIGALLGIPSLRVRDDFLAITTIGINFIVRSI
                     FLYVPFFGGALGIGGIPTPILFATKLRGQYYLALVAVLLALVTWFTFLFVRTWTGLAT
                     EAIREEETAAGAMGISHVRFKILAFTIGSAMAGLGGVLYAHFVNFISPDDFGFPVSIM
                     LLSMAVLGGMGSIWGPILGAIVLGSLPELFRPLVQYRMFLYGAALLLVIRFQPQGILG
                     EGSVLSRLVSRGRR"
     gene            complement(11306..12175)
                     /locus_tag="PLM30_00845"
     CDS             complement(11306..12175)
                     /locus_tag="PLM30_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR53745.1"
                     /translation="MQLLTEHLINGLAVGSIYALMALGLAMVYGILRILHVAHAGVYA
                     IGAYVGLYTFQATDSFLVAIAVAMAACALVGVAIEKLVYYPLLKFPPYVPLIASIAIF
                     LSIEELCRIVAGPYILGFPRRLALNSLTLGPVSLGGMQILVLLTSLVILLVLWFISDK
                     TEVGLAMQATSQDMAMASAMGVDTKRIISITFALGSAVAAVAGVLVGIYYNQVYPTMG
                     AVPAYKTLAIIVVGGLGSIPGAVLAALLLGLAEALLIGYAAIPLPRDALAFIAMIIVF
                     MIRPQGLLGKKTR"
     gene            12410..13084
                     /locus_tag="PLM30_00850"
     CDS             12410..13084
                     /locus_tag="PLM30_00850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR53746.1"
                     /translation="MKNYYSGKLNAALLMEVYRTDLERVRQYLQAEISYVAGYLTGAD
                     RVLELGAGYGRILKEIAPFAASVLGLDISVDSVLFGREYLKDIPNCRLETADAHLIKY
                     RSEFEMVLCLQNGLSAMKGDPLNLVTRGVNALVKGGKAFFSTYSDKFWGPRVEWFEEQ
                     ANKGLLGEIDRERTKDGKIVCRDGFTATTFSRDDLDGMGRAAGLPFMIEEVDESSLFL
                     VIDRRE"
     gene            complement(13118..14152)
                     /locus_tag="PLM30_00855"
     CDS             complement(13118..14152)
                     /locus_tag="PLM30_00855"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOR53747.1"
                     /translation="MKKMSEPANIDWENLGFRYMKTDFRYISKWKDGDWDEGRLVEDN
                     QICISEASSVFHYGQGCFEGLKAYRTQSGTIQLFRVDQNAKRMQESCRRGIIPPVPVG
                     KFIDACLRVVKANKTYVPPYGTGGTLYLRPFVIGVGDLIGVSPAPEYIFSVFCTPVGA
                     YFKGGLTPVNFTVSGYDRAAPFGTGAAKMGGNYAASLMPHELAKRRGFADCVYLDPAT
                     HTKIEEAGAANFFGITRDGRFVTPCSPSILPSVTRKSLLEVARGYLGIAAEERDVFID
                     RLDELSEAGACGTAAVITPIGGIEYRGRLHVFYSETEVGPVTRKLYDTLTGIQFGDIE
                     APEGWIFKVE"
BASE COUNT         2807 a         4345 c         4174 g         2830 t
ORIGIN      
        1 ccataggcaa gtccgccatg tccgcccttc tggcgacctt cgggctttcc atgctcctca
       61 agaacgtatg cctcaaccgt ttctcaccca acttcaggat cctgcagcac acgctgctgg
      121 gaggaaagac cttcaacctg gcgggtatca tcatcccgct gccccaactg gtcatcggcc
      181 tcgtcgccct tgccctcttg tacctgatat acctgctcgt caagaagacc cgcctggggt
      241 gggctatcca ggcaacggcg atggacaagg aagcagccga attgatggga ataaacactg
      301 aaaggatcta cctgctcgtc ttcggcctgg gcggggcctg tgtcgggata gcgggaggcc
      361 ttctgccatc ctacctcgcg gtccaccctg aagtgggagg gcttttcagc ctcatcgcct
      421 tcgtctgcgt cgccatgggg gggttcggca gcatcccagg ggcacttctg gcttcgcttc
      481 tgatcggcct cgttgaagcc tttgcaggct tttacatcgc accggtcttc aagtatgtcg
      541 ccgtattcgg gctctacctg gtggtagtga tggtcaggcc ccggggactc ttcggatggt
      601 gacggccatg acccttacag ggaaaaggac gaagagggac ctgttctggc tggggctcct
      661 ggcccttttc gcggtgatcc cgttgacgcc ctggttcact cgcagcacct tcgcgcagca
      721 cgtgatgatc caggtgctcc tctacgcgag catggcccag gcgtggaata tcctgggcgg
      781 ctattgcggc caggtctcct tcgggcattc cgtgttcttc ggcataggcg cctacggtac
      841 cgccatggcc gtcgtgaaat tcggcctcat cccttggccg ggcatcctgg cgggggcggt
      901 gatagcggcc gtcgtcgccg tggcgataag ctacccctgt ttcaaactca aggggcatta
      961 tttcgccata gccactttcg cgatagtgga gatcttcaac cgcctgttca tggtctggta
     1021 ctgggtcggg ggggccctgg ggctcgatta cgggttcacc cagccgggat ggaagaattt
     1081 cgtctggtac cagaccaagg attgctgctt ttacggcgtt ctccttcttt tcatcctggt
     1141 tttcggcatc gtccgctgga tagaaaagca ccgcttcggt ttctatctta gggccgttag
     1201 ggaggggcag gacacggcgg aatcactcgg cgtcgacagc accagggtga agttatcggc
     1261 catggccctt tcggccttcc tggccgccct ttgcggcgcc ttcttcgccc agtacaacta
     1321 ccgcgtggac ccgcccatgg tcatgtccct cgatatgtcc atgaagttcg tgctcatcac
     1381 gatcctgggc ggcctgggca ccttttacgg acctctcctg ggggccgctc tccttattcc
     1441 cctgcaggaa tatacccgcg cctacctcgg cgggatcgga ggaggcgtgg acctgatcat
     1501 tttcggcctc ctcatcatca tcgtggtcgt ctaccagccc aagggcatca tgggcattct
     1561 tgaccgggtt tacgtgaggt tcagacggtc aagggaagga gggaagtaac atggcccttc
     1621 ttgaagtgcg caaccttacg atgaagttcg gctcactggt cgcttgcaac gatgtctcct
     1681 tcgacgttga gaaggggtca gtcgtcgggc tcatcgggcc caacggcgcg gggaagacga
     1741 ccctcttcaa ctgcgtgtcg ggtttttaca agcccacgtc gggcaggatg ttcttcgccg
     1801 gccgtgaggt cacgaacctg ccggcccacg aggtggcccg ccgcggggct gtaaggacct
     1861 tccaggtggt ccgcccgttg aaagaaatga ccgtcatgga caatatcctc gtgggggctt
     1921 tcctgcgggg agccgatgcc ggtacggccc gagaggtcgc aggggaatgc ctccgcctct
     1981 gccgccttga ggaaatggcc gataacctgg ccgggggact ccccaccggt tacaagaaga
     2041 ggctcgaaat ggcccgggcc ctcgccacca agccgagcct gctcatgctc gatgaggcga
     2101 tggccgggtt gacgacgacg gagatcaggg aatcgatcga aataataagg ggcctgaaaa
     2161 aaggaggcgt tacccttctc gtggtcgagc atatcatgga agcggtcatg tccatcgccg
     2221 acaaggtagt cgtcctggac agcggggtga agatcgcgga aggcaccccc cggcagatag
     2281 tggaggacga gaaggtcata acggcctacc ttggggccaa gttcgccgaa aggatgaagc
     2341 gaacgggagg ggtgacgagg aatgactgac cctatcctga gagtccaggg ctttcattcc
     2401 gggtacgacg gcgtgcccgt catacacggg atagacctcg aggtggccga gggcgaactg
     2461 gtggccatcg tcggcgccaa cggcgcgggc aagaccacca ccatgaggac catcgccggg
     2521 ttgatgcacc ccttcaaggg gtgcgtgctc ttcgagggtg aggacatctc ccggaaaaaa
     2581 gcccatgaga cgaaccgcat gggtataagt tacgtccccg aaggaaggcg gcttttcccg
     2641 aaactgacca tcgaggagaa cctggagctc ggcgctttca ccaagaccga tcgggaggag
     2701 atcgaacgaa accttgagca ggtcttcgat cttttcccaa aactgttcga tcgccgaagc
     2761 cagatcgccg agaccctgag cggcggcgag caacagatgg tggccatcgc cagggggctt
     2821 atgtccaacc ctcgcctgct ccttctggat gagctctccc tggggctaat gccctccctc
     2881 gtggaaaagg taatggaggc tgtggtgtcc atcaaccggt caggggtgac cattctcctg
     2941 gtggaacaga tggtacagga ggccctggag atagccgaca ggggatacgt tctccagacc
     3001 gggaggatcg tccagcaggg gacggccagg gaactgctgg actcccccga ggtccgcaag
     3061 gcttatatgg ggatgtgacc cgggcgacct cgcgcaccgg agtaccgagg cccttggcgg
     3121 tcggcgagct cctggaccgc cgagggcttt aagccggagg tgttacggta tgggaggcaa
     3181 cgcgcagatc tacatcgaac tggagggcat cacacctaag atatggagaa gggtcgtcgt
     3241 cccctcctcc atcaccctcg accggttgag cgacgtgatc cagatcgtta tgggctggtc
     3301 gggagagcac atgcaccttt tctcgataaa gggcaggcaa tacatggacc tgcccgaaga
     3361 tgaaccggag gctcacgagg aaggtctctt caggcttggg tcgttggtct ccccgaagga
     3421 cggcgccttc acctacacct acgacctggg ggacaactgg acacacctgg tgaaggtgga
     3481 aaaaatgggc ctggaagagg acgaggaatg ggggaacctt ttctgcgtcg acgggaaggg
     3541 gacatgcccc cctgatgacg tgggcggtgt tcccggtttc cttgaattcc gccaggccat
     3601 ggccgacagg aaaggctccc agtatgaagt cttccgtgac tggtacgggg aggagttcga
     3661 ccctgacgcc ttcgaccagc tcgaggccaa cattacactg ctcttcttta tccgttggtc
     3721 cagggaacgc ttgctgccct gggaggagga ctgaccccct aggagaattc gaggtcccat
     3781 cgctcctcgg tgatatctct tccccaaagg aaatgggtct tcgtctcggt cagcctgaaa
     3841 ccgaagacct ggtaaagatg tcttgcggca tgcagttcac tgaccgtcca caggaaaacc
     3901 cttccatgac ccctgtcccg ggagtattca agggccgtgt ccataagggc ccggccaagg
     3961 ccaagacctc tgaaggccgg ctccaggagg aaccacctca actgtcccac ggcggccgaa
     4021 gtctcgacga tagcgatgga accgaccaca ttgcccccgc aatcggcgac ccacacctcc
     4081 cccttcccac ccaggtcgtg gagcaactgg gccatccccg ccaggagata gtattcaaag
     4141 gtttcgtcga aaccgtactc tttcttgtag aggacacagt gcctccagga taccaggccc
     4201 agctcccccg ccctgtaggg ccgcagaaag accttcccag catcggccct tccccggatg
     4261 ccgtcgaggc attcaattat ctgccttcgc ctcccgggcg aaaggctttc cagtattatt
     4321 tccccagttt tcatcggtcc gcccccttcg gatacttcgc ccacccacca ggccggtttt
     4381 cgtctttagg ttatatcaga acctctcccc cttccactcc gggaagtatt tctgatttac
     4441 gccgacttgc cttccatgtt atcctgattc tgtaacggcc gtgaccacca ggacccacaa
     4501 tctgccgaag gacaggggta tcttcatgaa gggtttttac aagcgttttg ccagtctcat
     4561 cttcggcttg ttcctctatt cgctcgggat agccataacg atgaaagcca acctgggcta
     4621 cgccccatgg gacgttttcc acaagggcat ctcgaacatg acggggataa ccataggcaa
     4681 cgccagcatc ctcattgggc tcatcatatg cgtgacggtg gcgctggcag gtgaaaaact
     4741 cgggttggga acgatactca acatgatcct catcggcttt tttctcgacc tgatcctcgt
     4801 cgtggacaaa atccccttga tgaaaggttt ccttcccggg gtggccatga tgaccttcgg
     4861 acttttcgtc atctccatcg ccacctactt ttacctgggc tccggttttg gggcggggcc
     4921 gcgggacggc ctcatggtgg tcctggaaag gaggacggga ctgcccgtcg gcctctgcag
     4981 ggctatcctg gagtcgatgg ctgtgttcct gggttggttg atgggtggtc ccgtgggcct
     5041 ggggacggcc ttcgcggctt tcgggatagg tttctgcatc cagataacct tcactctttt
     5101 caggttccgc gctaccgaag tcaggcacga aacgatcagg gaaacgctga gggatttcag
     5161 gctcagccat cgataaaggg aaaacccatg ccctgttcag gggcggaaag ttcacccccc
     5221 gggagggcat tctcccggag gggtcggagc atggagttgg ggcggtaaag gcatgccgat
     5281 caggattgtt ttccatggcg aattgaccgg cctactgaat aaccggggcc tttcacccga
     5341 agcgacacgc gatcttttcc gggcggcccc tgtcaaggac ctcatcgaat cccagggagt
     5401 accccatacc gaggtgggcc gcataacatc agagggcctg gaaaggccgt tctcgcacgt
     5461 cccggcctgg ggggagaccg tggaagtcca cccccacagg ccacccgttg acgtgtccct
     5521 ctcctcggtc ctgttcccgg aaccctttga agaggtcggc ttcgtggccg actcgaatgt
     5581 ggcccgcctc ggcggccttc tcaggctggc ggggttcaac tgtctcctgg acccccgttg
     5641 ggacgacgcc cggatcgcgg aaacagccgg cgctaacaaa agaacccttc tcaccaggga
     5701 caaggaactg cttaagcgca acagggtcat taggggccgc ctcatccgcg cggaacgtcc
     5761 ctgggaccaa ctggccgagg tgatccattt cttcgggctc agcgacaaga tcgacctctt
     5821 cagccgttgt cccaagtgta acgtcctcct ggaagggacc gaaaagagcg ccgtcatcga
     5881 ccaactggag cctctgaccc ggatgatgta cgaccatttt acccggtgcc ccggctgcgg
     5941 acaggtctac tggtcaggct cccaccacgg ccgaatccgc gaaaaactgg cggcggcgct
     6001 ggagcaggct tcaaaggact gaccccccgc agaaaacagg ataagtcctc actccagctc
     6061 cttcaacacc ccctccagaa tgtcggcggc cttcatcaag tgcgcctcgg agataaccag
     6121 cggcggcagg agcctggcga cgttgttgtg gtgaccgcca acttctatca tcacaccccg
     6181 ctggtgggcc agttttctca gctgcttcgt caggcccccg gccggtttcc tggtcttctt
     6241 gtcctcgacg atctcgagag cgatcatgag gccgatcccc ctggcctcgc ccaggatcct
     6301 ggaccctttc tccatctccc tgaagcgggc catagctttt tcccccagtt gggcggcata
     6361 ctcggggacc ctgttctcga tcatccattc aaggcccgcc gccccggcgg cgaaggccac
     6421 catgttgccc ctgaaggtgc cgatgctctt cccggggggc agggtgttca gttcttccct
     6481 gaaggcgatg gcggaaatgg ggaatcccgc ccctccgagg gccttgctca tggtgacgat
     6541 gtcggggaca attccggaat gctcaaaggc gaacatttta cccgtcctgc cgaggccgca
     6601 ttgtatctcg tcgcagatca gaagaatgcc cctggcttcg gtgatctccc tgaccctctg
     6661 gaggaaaagg ggaccgggta ttatcgtccc cccctcgccc tggacggctt caaggagcac
     6721 cgccgcaggt tttgaataac cggagtgggt gtcatcgagc accctctcga ggttgtcggc
     6781 ggcgaactcg tcgaccttct cctcgggaca gccgaagggg ttcctgtacc tgtagggaaa
     6841 ggggaggaac tggaaaccgg gggccaggga gccgatgccg tccttgtggc cgctgtcgct
     6901 ggtggcggcc agggatgccc ccgtcatgcc gtggtaggcg ccttcgaaga caattatccc
     6961 gtccctgcgg gtgttgaacc gggccagctt tatcgcctgc tccacggcgt cggaaccggt
     7021 gggccctccg aagaaaaccc tggagagctc cgccgggagg atgcgcccca ggaccttgac
     7081 caacctcatg cgggtctcgc tcgggatatc tagtgtgtgg gtgaccttat cgagttgccc
     7141 gtgggcggcc tcaaggacaa aggggtgtgc gtgacccaac gccatgaccc ccgctcccga
     7201 gaagcagtcg atgtagacat ttccgtccac atcctcgagg gtggcacctc ttccccgggc
     7261 cagggccatg ggcatccccc tggggtagga gacggcggag ccctcaagct cttcctgtat
     7321 cttcaggatc tccagagatc ttggccccgg cggggtcacc aacatccgcg gagcgtcctt
     7381 gacggataga tccacgaact tctcccagtc cattatcgat cctccctaca atattttctc
     7441 tcctggttcg agggcgcagg gatcaccccg ccaagacacc ggcgaccgtg acatgagctc
     7501 ttcatagcgg ccgttcttcc caatttcccg accaacgcga gccatcgccg gaccctccac
     7561 cgcgcccagg atgaaagggg cgaaaccaaa ggccaacggc gaaaaaaggc cgggggaacc
     7621 tcaccccggc cttgcgactc ccttcccctt cgaccctggg gcctttggga acgggttttc
     7681 tcggttattt cctcgggtca ataacttcgg ggtcgtccac tattccgtaa cggcggaaaa
     7741 ccccgtcctt gactatctga acctggaccg gtttgatgac ctccccgatc tcggtgaagc
     7801 ccttgatgac gcccgttacg ccgtcgaagt ccttcaaagc ggcgatggcg tccttgacct
     7861 tgtgcggatc ggtggtcccg gcacgcctga tgccgtcgac gatgatgagg aacgcgtcgt
     7921 agttggaggc tcccgtcatg tcgggctcca tgccgtaacg ttcgcggtat tccttgatgt
     7981 aggcctgggc cacgggcctc tcgtcgtcgc ggtcgaggtt ggtcacaatt atcgtcccgt
     8041 tggcagcctc agggcctgcc agctcgatga acttgggtga atcgtaacct tcttcaccga
     8101 tgatctgggt cctgagtttt atttcgtggg cctgcttcac tatcccggcc gcttcggcgt
     8161 aatagccgct ggccaggatc acatcagggt ccgcctcctt gacggccgtc aggtagggca
     8221 tgaggtcctt ctcgccaagt gggtaaacct ggctgtagac caccttggcc cccttggaga
     8281 ccacgtagga cttgaacccc tcggccatct gcctgccgaa gtcgttgtcc atggtaagca
     8341 tacccacggt cttcgccttc agcatgtcga cggcggccac acctgccccg cggccctcaa
     8401 cagtcccgag aaaaccgttc ctgaagcaga aatcaccggc cgcggtgata tcggggtgga
     8461 cggcgtagcc ggccaccagg gggatctcga actcctggaa gaggggcgcg accgcacggg
     8521 tgggcatgct ataggaaccg cccaccaccg cgacgacctt gtccatctgg acaagtttcc
     8581 tggcaagacc gatggcttcc ttggcctcgc cggcgtcatc ctcgtagaac agctcgatct
     8641 gcttgccgag gactcctccc ttggcgttga cctggtctac cgcgagctgg acggactgca
     8701 gaacggccag accgtcggcg gcggcgtacc ctgtcagagg ggcaagcagg cctatcctga
     8761 tcgtgtcagc ggcggaagca gctccgaccg ctccgaagac caggaccagc gcgaggatca
     8821 gcatcaccgt tcttttcatg aaaaaccctc cttttcggtc ctatatgtac aggcacttac
     8881 aagacccgcc atccctggct caaacactgc ctccagcggc atcacctccc aggtaagctt
     8941 cccggatctt tgggttttgg cttagatccg aagccttacc ctccagggct atacgtccgg
     9001 tttcaaggat ataaccccta tgggctatcg ccagggcctt gaccgcgttc tgctccacca
     9061 gcaggatggt cagccccttc tcgtcgttca atcttttcag gaccgagaag accaggtcca
     9121 ccaacttggg catcagcccc atagatattt cgtccaccag gaggatcctt gggcttgaca
     9181 ccagcgcccg ggcaatggcc agttgctgct gctcaccccc ggaaagggtc tgccccgcct
     9241 ggcgtctcct ttccttcagt acggggaaga gttcgtagac ctcgccgaaa gcccgttcca
     9301 catcgacgct gcccgagagg ccgtagaccc ccattctcag gttctgctcc accgtcaggt
     9361 tggggaagac cctggccctt tcaggcacga tcctgatccc cagggccgct ctttcatggg
     9421 cgggaagctg caggatatcc cgccccccgt tacccccgtc cttagagggt tcaacataaa
     9481 gcgccctgcc ggacctggcc tttacaaggc ccatcagggt attcaaaagg gtcgtcttgc
     9541 cggcgccgtt ggcgccgatt atggatacca tctccccggc ttccaggaag aaggagacat
     9601 cttgcagtgc caggatgtct ccgtaggcaa cggtcaggtt ctcgacttca agcatggcct
     9661 tattccccct tccctaggta ggcctcgata accttctcat cggacctaac ctcgtcgggg
     9721 gtgccttccg ccaatttgcc cccgtggtcg agcaccacga gcctctcgca cagcgacatg
     9781 gccaccctca tgttatgctc gatcaggagg atcgtgaatc ccctttcctg gattttcctg
     9841 accaacccca tcatccgccc gacctcctcc tggctcaggc ccgagaagga ttcgtcaagg
     9901 agcagcagtt taggccccag cgccagggcc ctcgccaatt cgagacgcct caggttcccc
     9961 agggaaagga tggaggccgt gctgtcggcg aagccttcga gacccacctc attaaggaat
    10021 gtcatggcct gcccgcggtt cttgctgttg ttgaaaagag ggctgctttt gatgaaagac
    10081 ccgtacctcg gcatgcccag tccgaccatg acgttgttca gtaccgtcat cccgccgaag
    10141 ggcctcacta tctggaaggt gcgccccacc cccctggccg cgatggaatg ggccggcagg
    10201 ccgtcgatac gcctgccgtc gaggaagatc tgtcccgacg tagggcggta taccccggaa
    10261 atgacgttga agcaggtggt cttccccgcg ccgttgggtc cgatcagtcc caggatctca
    10321 cccctgcgca catgaaagga gacgttgtcc accgctttaa gtcccccgaa atggcggctc
    10381 atcttctcca cgcggaggat caggtcctcg ccgggcatgc tcatcgcctc ccccgggaga
    10441 cgagtcggct taatacgctg ccttcaccca gtatcccctg gggctggaac cggatgacca
    10501 gcaaaagagc ggcgccgtaa aggaacatcc ggtactggac caggggcctg aagagctcag
    10561 gcagggagcc aagaacgatc gcgcccaata tgggccccca gatgctcccc atgcccccga
    10621 gcacggccat ggagagcagc atgatggata cgggaaaacc gaagtcgtcg gggcttatga
    10681 aattgacgaa atgggcgtaa agcacccccc cgaggccggc catggcgctt ccgatcgtaa
    10741 aggcgaggat cttgaagcgt acgtgggaga tgcccatggc cccggcagcg gtctcctcct
    10801 cccttatggc ctccgtggcg agaccggtcc aggttctgac gaaaaggaag gtgaaccagg
    10861 tcaccagggc cagcaggacc gcgacaagag ccaggtagta ctgccccctg agcttggtgg
    10921 cgaagaggat cggggtcggg atacccccta tccccagggc cccgccgaaa aaggggacat
    10981 aaaggaagat gctcctgacg atgaagttga tgccaatggt ggttatggcg aggaaatcat
    11041 ccctgacccg caggctgggt atgcccagca acgccccgat catgccggtg atcagcgtgg
    11101 ccagagggag gctccaccag aaagacaagc ccgccttcgt gcaaagcaag gccgaggaat
    11161 aggccccgat gccgaaaaag gcggcgtgtc ccagggagat ctgcccggcg aacccgacga
    11221 tgatattcag gccggaagct accagggcgt agatgcctat catgatggcc acggtcgaga
    11281 agtaaccacc catcgctcca gccccctatc tcgtcttctt gcccaggagt ccctggggcc
    11341 ggatcatgaa cacgattatc atggcgatga aggccagcgc gtccctggga agcgggatgg
    11401 ccgcgtaacc gatcagcagg gcttcggcca ggccgagcag caaggccgcc aggacagccc
    11461 ccggtatgct ccccaggccg ccgaccacga tgatggcgag ggtcttgtag gccggcacag
    11521 cacccatggt cgggtagacc tggttgtaat agatccccac caggaccccc gcgacagcag
    11581 ccacggcgga gcccagggcg aaggttatgg agatgatcct cttcgtatcg acacccatgg
    11641 cgctcgccat cgccatgtcc tgggaggtgg cctgcatggc caggcctact tcggtcttgt
    11701 ccgatatgaa ccacaggacc aggagtatga ccaatgaagt gaggagcacg aggatctgca
    11761 tgcctcccag gcttacgggc cccagggtca gggagttcag ggccagcctc ctggggaacc
    11821 ccaggatgta gggcccggcg actatcctgc agagttcctc gatggagagg aagatggcaa
    11881 tgctggcgat gaggggcaca tagggcggga acttgaggag tgggtaatag accagcttct
    11941 cgatggccac ccccaccagg gcgcaggccg ccatggcgac ggcgatggcc actaagaagc
    12001 tatcggtggc ctggaaggtg taaagtccca cgtaggcacc gatggcgtag acgccagcgt
    12061 gggctacgtg aaggatcctg aggatgccgt agaccatcgc cagcccaagg gccatgaggg
    12121 cgtagatcga gccgacggcg agtccgttga tgagatgttc ggttaatagc tgcatattcc
    12181 accccactga ttgagatcgt cacccaagaa aaagccccga atcccgatgc cggagggcga
    12241 aaaaagggat gcttttcttc agggttgcat ctcccgaagg accgagaagc accggtgacc
    12301 ccggagagtg gacctatcga tcgaacatct taccgtttta cgatatcatt gacctgcgaa
    12361 cagtcaagtg tcatctcgaa ctccaaaaga ccccgtggaa ggtgaggtcg tgaaaaacta
    12421 ctattccggc aagttgaacg ccgccctgct gatggaggtt taccggacgg acctggagag
    12481 agtgaggcaa tatcttcagg ccgagatatc ctacgtcgcc gggtacctaa ccggtgccga
    12541 tagggtgctt gaactgggag ccggctacgg gaggatactg aaggagatag cgcccttcgc
    12601 tgcatccgtt ctcgggctgg atatttccgt ggattcggtc ctcttcggga gggagtacct
    12661 caaggacatc cccaactgca ggctcgagac cgccgacgcc catctcatca agtaccggtc
    12721 cgaattcgag atggtcctct gccttcagaa cggcctctcg gcgatgaaag gagaccccct
    12781 gaacctggtg acccggggtg tgaacgccct cgtcaagggc gggaaggctt ttttcagcac
    12841 ctatagcgat aaattctggg gccccagggt ggaatggttc gaagagcagg caaacaaggg
    12901 tctgctgggg gagatagacc gggaaaggac caaggatggc aagatcgtct gccgggacgg
    12961 ctttaccgcc acgaccttct cgagggacga cctggacggg atgggccgtg ccgcgggcct
    13021 tcccttcatg atcgaggagg tcgatgagtc cagccttttc ctggtcatcg atcgaagaga
    13081 atagtttgag ctgaccaagg ggcacctggc gaggcctcta ctccacctta aaaatccatc
    13141 cttccggagc ctcgatgtcg ccgaactgga tccccgttag ggtgtcgtaa agcttcctgg
    13201 tcaccggccc gacctcggtc tcgctgtaga agacatggag cctgcccctg tactcgatcc
    13261 cgccgatggg agtgataacg gcggccgtcc cgcaagcccc ggcctccgag agttcatcaa
    13321 gcctgtcgat aaaaacatct ctctcctcgg ccgctatgcc gaggtacccc ctagccacct
    13381 ccaggaggga cttcctggtg acgctgggga ggatcgacgg cgaacaggga gtgacaaacc
    13441 tgccgtccct ggtgatgccg aagaagttgg cggcccctgc ttcctcgatc ttggtatggg
    13501 tggccgggtc aaggtatacg caatcggcga aacccctgcg cttcgctagt tcatggggca
    13561 tcaggctggc ggcatagttg ccgcccatct tcgcggctcc ggtaccaaag ggggccgccc
    13621 tgtcgtaccc cgaaacggtg aaattcaccg gcgtaagacc tcccttgaag taggcgccga
    13681 cgggtgtgca gaagacgctg aagatgtatt ccggggccgg ggagacaccg atgaggtctc
    13741 ccacgcctat gacaaagggc ctgaggtaaa gggtgccgcc cgtgccgtag ggcgggacat
    13801 aggttttgtt ggccttcaca acccgcaggc aggcgtcaat gaacttcccg accgggaccg
    13861 ggggaataat acccctgcgg cagctctcct gcatcctctt cgcattctgg tccaccctga
    13921 aaagctggat cgtcccagac tgtgtcctat aggctttgag cccctcgaag cagccctgcc
    13981 cataatggaa aacgctggaa gcctcactta tgcagatctg gttatcctcc acaagccgcc
    14041 cctcgtccca gtcgccgtcc ttccattttg aaatgtaacg gaagtccgtc ttcatgtacc
    14101 tgaaccccag gttttcccag tcgatgttcg ccggctcact catctttttc accccc
//
LOCUS       DAOSRG010000010        13979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4446_length_13979_cov_9.436441, whole genome shotgun sequence.
ACCESSION   DAOSRG010000010 DAOSRG010000000
VERSION     DAOSRG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4446_length_13979_cov_9.436441"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..997
                     /locus_tag="PLM30_00860"
     CDS             23..997
                     /locus_tag="PLM30_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOR53748.1"
                     /translation="MDGLRVGTCSWTDRSLIASGWYPDNSRDAYSRLKHYANFFDTVE
                     VDSTFYAIPGEEVFYQWAARTAPGFLFNIKAFGLFTFHTLSVTALPRELMPPGISKGS
                     RLRIWEIPKDTRIALWERFISRVLVLHSMNRLGYLLFQFPPWVVFSERHLNWFKRVAT
                     LASPARVAIEVRHRSWLEEGNRERFLDLLRDQNMAFTAVDEPSLEWTVPPEWPITATW
                     GTVMRFHGRNAPAWDKRDAKVAERFNYLYDINELAPFSEKARAFAKDGGRVFLMFNNC
                     FRDNAVRNALEMKALLGVDAGGRIQGQEMLNLWDMSPGGASAGGSEEP"
     gene            1053..1850
                     /gene="folE2"
                     /locus_tag="PLM30_00865"
     CDS             1053..1850
                     /gene="folE2"
                     /locus_tag="PLM30_00865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOR53749.1"
                     /translation="MRDVQSERDMRNIPIDSVGVTGLAYPIEVRDRNEKTQHTVAMVE
                     MSVSLPRHFRGTHMSRFIEILNSCNGHVTLQNLEHIASALREHLEADKAELKFSFPYF
                     ATRRAPVSGAPSLTRYDAVFWASCGEDFDFTLTVTAPVNTLCPCSKEISERGAHNQRA
                     EVMITVRMDHLVWIEELADIAEESASSPVFTLLKREDEKYVTERAYDNPRFVEDVVRE
                     VAVRLDDDPRISWYSVRVVSHESIHAHEAFASLSRDKRLRNDPGRTG"
     gene            1850..2803
                     /locus_tag="PLM30_00870"
     CDS             1850..2803
                     /locus_tag="PLM30_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOR53750.1"
                     /translation="MKILFSPKTFEKLRDVIGPLLAGHEMEVADEADLEDRLGEAEVL
                     VCGPLDVGEALLKGAPRLRLVHQWGVGVDRIDLEACRKMGVAVCNVPSGGTGNAEGVA
                     EIALMHLLLHSRKYARCQESIRKRRLFSPQGVSLWKKRACVVGLGNVGRNVVSRLKGM
                     DMEVRGVNRTERESFGSLGLDGFYRLDSVEEALSGCRFVVLSLELNEETREVASGAFF
                     KAMEPGSFLVNVGRAELVSRRALEGSLESGHLAGAGLDVFWDEPADPDDPLLANPKVT
                     LTPHVGGVTDEALMGVAQTVADNISRLERDLDLLNRLGRER"
     gene            2800..3501
                     /locus_tag="PLM30_00875"
     CDS             2800..3501
                     /locus_tag="PLM30_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOR53751.1"
                     /translation="MKHRRNPVRTGRDGTEAALFDFDMTLVDSSVGITYCLNRLAGFF
                     GLYEVTREEVTSTIGHPMDKAMEMLWGRYEPAWMDHYREYLVPLEYERIMPFPGTREA
                     LARLLDEGFSLGVVSNRKKLLRAVESAGLGEYFLSFVGMDDVENPKPHPESIWLSMSN
                     LGSTASGTTLVGDSEIDALAAGRAGIGFVGVSSGGASPDALSSAGALVVIKDISELAW
                     ILPGRTGRRLQCGPG"
     gene            3514..4437
                     /locus_tag="PLM30_00880"
     CDS             3514..4437
                     /locus_tag="PLM30_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53752.1"
                     /translation="MKGKTATKAFYYFFTVCIVLGAILVYKAWWDRYFRLRPELAVAE
                     PVALSLDIPLEGVLIWEEVVMASPAEGPVSYPRGMGPAYCARGDVVAEVASPAGRRQV
                     KAPSPGYFMAALDGAEGGWSYGVLWPGSSRLPEPGPARFLHEGQRVSLGGGIGKFIPQ
                     PQTLRCVAYVEKSALGWNPADRDLLEVKRTASDLSFRAEVRAVNDLGPIYKVYLTFPF
                     FSSADVLSRKVSYLLHAEDVRGVLIPESAVTLRDRKKMAFLVTGERSIAVEVTGIPVS
                     GKRFLVKKGLEPGDLVIASGADAAEGEVRIW"
     gene            4434..>4624
                     /locus_tag="PLM30_00885"
     CDS             4434..>4624
                     /locus_tag="PLM30_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOR53753.1"
                     /translation="MSTIADNIASVRVRIARAATRCGLEGDRVDIVAVTKTRSIDEME
                     EAARCDVIALGENRVQEALS"
     assembly_gap    4625..4724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4725..4899
                     /locus_tag="PLM30_00890"
     CDS             <4725..4899
                     /locus_tag="PLM30_00890"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR53754.1"
                     /translation="TRMLFASMRELRDETESRLGLSLPVLSMGMSGDFEYAVMEGSTM
                     VRIGSAIFGGRIN"
     gene            4923..5420
                     /locus_tag="PLM30_00895"
     CDS             4923..5420
                     /locus_tag="PLM30_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOR53755.1"
                     /translation="MSDLLTVLDIERISFNKAMRGYNPQEVEEFLEKVAESLQVYAEK
                     VKTLEVQLSRFDEQIQEYAELKQSLQEALVLAQKSAEERVSSAEGKAEAIIAESEAQA
                     HQIVMSAKDRVDTHKRDIQRLRQVRYQFVAEFKGLLAKFGSLLNSLEAPVEEEEGEKE
                     GESHR"
     gene            5417..7492
                     /gene="recG"
                     /locus_tag="PLM30_00900"
     CDS             5417..7492
                     /gene="recG"
                     /locus_tag="PLM30_00900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOR53756.1"
                     /translation="MTEGLTRDTPVRFLKGVGPVKASRLLTLGVQTVEDLLYLFPRRY
                     ENRGVAAPLGSLQAGSFSSAVATVLAIERKPTRRRNLTLVTALLSDGSSLAQAVWFNR
                     KGLERVLTPGTKASFYGKVERRGGLLQYTNPEFEVLADEDDPSDQRTVVPVYPGTEGL
                     YQKWLRRLISDALEAFPGDLVDPLPPSIREAKGFMSRVPAVAQMHYPSGREEWALARK
                     RLAYEELFLLQVALAIRKQGYREETGGEPLEWEGPLMKHFREGILPFSLTRSQEKVLG
                     ELAADSSGNVPMNRLLQGDVGSGKTAVAVIFLIAAVDSGFQGAIMAPTEILARQHFKR
                     ISGWLSALGVPVHLLTGSLPDPQREGILADIASGEPCIVVGTHALIQKTVRFGRLAAV
                     VVDEQHRFGVLQRGALTAKGSHPHVLVMTATPIPRTLTLSVYGDLAFSVIDELPPGRT
                     PTETRYLRDPDDPRLARFIANETAQGRRVYWVCPVIEESDKLPLMPVIRRHETLSKRF
                     PGEKVGILHGQLPPEERQSTMERFVSGELSILVSTTVVEVGVDVRDATVMVIEDAHRF
                     GLSQLHQLRGRVGRGEVGGFCFLVGKPGTEDGRARIEAMRTSSDGFEIAERDLALRGP
                     GEICGVRQHGITDFKVADLVKDRDLLEEAREDAFTLVGSDPVLSGYPDLRREVFRKLG
                     TKLKLAGTA"
     gene            7496..7939
                     /locus_tag="PLM30_00905"
     CDS             7496..7939
                     /locus_tag="PLM30_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOR53757.1"
                     /translation="MKRDDSFEGLLEYAMRLISRSEVTCSQLKRKLVARGASSRTSEK
                     VCRELKESGYIDDRRYCELFISTHGEYGFKRMKVELLKRGIERDLVESMVAFDQEDEV
                     GKALAMICHWGPDLEMEKAWGRLSRRGFSYPVIREALRRTCGEAS"
     gene            8037..9566
                     /gene="rny"
                     /locus_tag="PLM30_00910"
     CDS             8037..9566
                     /gene="rny"
                     /locus_tag="PLM30_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOR53758.1"
                     /translation="MLPVILAAAFAVGLSIGFLFYKILSDRKLKGTREQSDQLLKDAF
                     QEADRRKREMLTEAREEIHRLRQEAEREIKERRAEQQRAERRLEQKEENLDSKMESIL
                     QREEEYRKKQEELASRLEDVESVRRKQMERLEEIAGMSREDAKDFLLKEVEEEAGHLI
                     GLRLKDLEEKARRESERKSREIVVSAIQRCAVDHTSEMSVSVVTLPSDEMKGRIIGRE
                     GRNIRAFETYTGVDLIVDDTPEAVTLSSFDPVRREVARLSLERLVLDGRIHPARIEEI
                     VEKAQRDVEEKILEAGEEALLETGIKSMHPELVKLIGQLRFRSSYGQNALSHSLEVSH
                     LSGIMAAELGIDEHVARRAGLLHDIGKAVDHQVEGPHALIGRDLAKRFGETPEVVNAV
                     AAHHEDQEMTSVYDVIVSAADAVSASRPGARRESLESYIKRLETLETLAKTFKSVTKA
                     YAIQAGREVRVMVAPNSLDDGSIHKLAYDIARKIESEMKYPGQIKVTVIRETRSVEYA
                     K"
     gene            9576..10349
                     /locus_tag="PLM30_00915"
     CDS             9576..10349
                     /locus_tag="PLM30_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOR53759.1"
                     /translation="MKVLFIGDIVGHPGRRALAGLLPRARTEWGPFDFVVANVENAAG
                     GFGLTEKVMEELFRQGLDAMTSGNHIWDNREALPLLGAESRLVRPANYPPAAPGTGST
                     VIEKKGLKLGLLNLQGRVFMQPIDCPFRRADEELLLLKEFPVLVDIHAEATSEKRALA
                     LYLDGRVSAVLGTHTHVQTADEGILEGGTAYISDVGMTGGHGGVIGMDKEAIVYRFLT
                     GMPTRFEVCKSDLRMDAVAIEIDEASGRSLSITRLSLDL"
     gene            10738..>11549
                     /gene="mgtE"
                     /locus_tag="PLM30_00920"
     CDS             10738..>11549
                     /gene="mgtE"
                     /locus_tag="PLM30_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR53760.1"
                     /translation="MKERLDRFYNQIESLMSEHRFTEIKETLSTMEPADIGEMLVEAP
                     QAERVLIFRLLSKDLAIDVFEHMEGAEREELLDCFTAKEAAEIIEEMSDDDRTALLDE
                     LPAKTVEKLMQHLSPEERKIANHLLNYPEDSAGRIMTAEYIGLKSSMMATVALQHIRK
                     VARSKETIYTCFVMDGERRLRGVVDLEDIILAQPETLVSDLMDDDPVFVTTLTDQEKV
                     AQVMSRYDLHVIPVVDLENRLVGIVTFDDVLDVVEEEATEDFERMAGIQPFE"
     assembly_gap    11550..11649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11664..12650)
                     /gene="hypE"
                     /locus_tag="PLM30_00925"
     CDS             complement(11664..12650)
                     /gene="hypE"
                     /locus_tag="PLM30_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOR53761.1"
                     /translation="MERLSIGHGAGGSLTQELIDQITGRFDGSPLADGEDCAILPGGF
                     AVTIDGYTVSPLSFEGGDIGKLSVCGSVNDLSVRGAKPLYLALGVIAEEGFPVRELLV
                     YMESAAAVCRSLGVELVTGDSKVVPRGQVDGLFLSTCCLGRMVREDPLGAVNLRPGDA
                     VILTCPPGLHGATIAAHRYGLKARGLSSDCAALWPMLESVLPVSGLRCMRDCTRGGLA
                     TVLCEWSERAGLGIEIDESLIPWDEAALSVCDLLGFEPLHMAGEGCAAVAVEEGEAQR
                     TVTLLKRHSLGERASVIGRVTKEHPGLVGIRTHSGGTRLVDRPVGELLPRIC"
     gene            complement(12638..13750)
                     /gene="hypD"
                     /locus_tag="PLM30_00930"
     CDS             complement(12638..13750)
                     /gene="hypD"
                     /locus_tag="PLM30_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOR53762.1"
                     /translation="MSLQAHRPGDPRKRVELLSKALSELADPPIKVMEVCGTHTVSIF
                     RQGLRTLLPAKISLVSGPGCPVCVTSQGEIDAALSLASREDTTIATYGDMLRVPGTET
                     TLAEERSRGADVRVVTSADEALSLAMNDRTRSVVFLAVGFETTAPATASVVLDAWREG
                     LDNFMVLDLHKSVPPALGALASDPDVNLDAFLLPGHVSVITGLATYRFLPERFGIACA
                     VAGFEAEGIMMALVEVARQIRMKRPRVASLYSRAVRPQGNVAARNVMDRVFTLGDAPW
                     RGLGVIPSSGFFLRKEFARFDAVERLGVAVKETPPPVGCRCGEVLKGLIDPPSCDLFG
                     RSCTPAHPVGPCMVSTEGSCAAYYRFQKRGYLDWKD"
     gene            complement(13731..13958)
                     /locus_tag="PLM30_00935"
     CDS             complement(13731..13958)
                     /locus_tag="PLM30_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOR53763.1"
                     /translation="MCLAIPHRVIALKGPNRALALAGPVEVEIRTDLVPHIKEGDMVL
                     VHAGFAIERLLEEETGELEKLWEEIRELAGP"
BASE COUNT         2933 a         3909 c         4374 g         2563 t
ORIGIN      
        1 atgatccccc ggaggtctag ctatggatgg cctgagagtc ggtacctgtt cctggaccga
       61 ccggtcgctc attgccagcg gatggtaccc ggacaattcc agggacgcct attcaaggct
      121 taagcactac gctaactttt tcgataccgt cgaggtcgac agtaccttct acgccatccc
      181 cggcgaggaa gtattttacc agtgggccgc gaggacggcg ccgggttttc ttttcaacat
      241 caaagccttc ggccttttca ccttccacac cctctccgta acggcccttc cccgggaact
      301 gatgcctcca ggcatctcca agggctccag gctgcggatc tgggagatcc ccaaggatac
      361 acgcatcgcg ctctgggaaa ggttcatctc ccgggtcctg gtgctccaca gcatgaacag
      421 gctgggttac cttctcttcc aattcccacc ttgggtggtc ttttccgaga ggcacctcaa
      481 ctggttcaaa agggtcgcga cactggcctc accggcgagg gtggccatcg aggtcaggca
      541 caggtcgtgg ctcgaggagg gcaaccgtga gagattcctg gacctcttga gggaccagaa
      601 tatggccttc accgccgtgg atgagcccag cctggaatgg accgtcccgc ccgaatggcc
      661 tatcacagcc acctggggaa cggtgatgag gttccatggg aggaacgccc ccgcctggga
      721 taaaagggat gccaaggtgg cggagcgatt caactacctg tacgatataa acgaactggc
      781 ccctttcagc gagaaagcca gggccttcgc gaaggatggg ggcagggttt tcctcatgtt
      841 caacaactgc ttcagggaca acgcggtacg gaacgctctc gaaatgaagg ccctcctcgg
      901 cgttgacgcc ggcggccgga tacagggcca ggagatgctg aacctctggg acatgtcgcc
      961 gggaggagcc tcggccggtg gttccgagga accataagcc ccaggaggga tccaggcata
     1021 gcgactaatt tacaggagag ggggtcttcc tcatgcgtga tgttcagagc gagcgcgata
     1081 tgcggaacat cccgatagac agcgtggggg tgacgggctt ggcctacccc atcgaggtca
     1141 gggacaggaa cgagaagacc cagcacaccg tggccatggt ggagatgtcc gtctccctcc
     1201 caaggcactt cagggggacc catatgagcc gtttcatcga gatcctgaat tcctgcaacg
     1261 gccatgtgac cctgcaaaac ctggagcata tcgcctcagc cctgagggag cacctcgaag
     1321 ccgataaggc ggaattgaag ttcagtttcc cctacttcgc cacgaggaga gcccccgtca
     1381 gcggcgcgcc cagcctcacc cgctacgatg ccgttttctg ggcctcctgc ggggaggatt
     1441 ttgacttcac cctgacggtg acggcgccgg tgaacaccct ctgtccctgc tcgaaggaga
     1501 tctccgaaag gggagcccac aatcagcggg cggaagtgat gataaccgtg agaatggacc
     1561 acctggtatg gatcgaggaa ttggccgata tcgcggaaga gagcgcctca tcccctgttt
     1621 tcaccctttt aaaaagggag gacgagaagt acgtcaccga aagggcctac gataacccca
     1681 ggttcgtgga ggacgtggtc agggaggtgg cggtgcgcct ggacgatgat ccccggatca
     1741 gctggtactc agtcagggtg gtgagccacg aaagtatcca cgcccacgag gcctttgcca
     1801 gcctttcaag ggataaacgc ctccgaaacg accccgggcg gacagggtaa tgaaaatact
     1861 tttttccccc aaaacctttg aaaaacttcg ggacgtcatc ggcccgctgc tggcgggcca
     1921 tgaaatggaa gtcgccgatg aggccgacct cgaggatcgc ctgggggagg cggaggtgct
     1981 ggtctgcggc cctctggacg tgggtgaggc cctcctcaag ggcgcaccga ggctcaggct
     2041 ggtacaccag tggggagttg gtgtcgacag gatcgacctg gaagcctgcc gaaagatggg
     2101 cgtggccgtt tgcaacgtgc cttccggggg cacgggaaac gccgagggtg tggccgagat
     2161 agccctgatg cacctgctcc tccactcaag gaagtatgcc cgttgccagg agagtatacg
     2221 aaagagacgg ctcttctccc cccagggtgt ttccctgtgg aagaagaggg cctgcgtggt
     2281 agggctgggg aacgtgggac ggaatgtcgt atccaggctc aaggggatgg acatggaggt
     2341 caggggagtg aaccggaccg aaagggagtc cttcggttcc ctcggcctcg acgggttcta
     2401 ccgactcgac agcgtggagg aggccctttc aggatgcagg tttgtcgtgc tctccctcga
     2461 actcaacgag gagaccaggg aggtcgcctc cggggcattt ttcaaggcca tggagcccgg
     2521 ttccttcctg gtcaatgtcg gcagggccga actggtatcc cggagggctt tggaaggatc
     2581 ccttgagagc ggccatcttg ccggcgccgg gctcgatgtt ttctgggatg agcccgctga
     2641 ccctgatgac ccactgctgg ctaaccccaa ggtcaccctc acgccccatg tcggaggggt
     2701 cacggacgaa gccctgatgg gtgtcgctca gaccgtggcg gacaacatct cacgcctgga
     2761 aagagatttg gaccttttga accgactggg ccgggaaaga tgaaacaccg gaggaacccg
     2821 gtccggacgg ggagggacgg gacagaggcc gcccttttcg atttcgacat gaccctggtg
     2881 gacagtagcg taggcatcac ctattgcctg aacaggctgg ccggtttctt cgggctgtac
     2941 gaggtgaccc gggaggaagt gacatcgacc atagggcacc ctatggataa ggctatggag
     3001 atgctatggg ggcgctatga acccgcctgg atggaccatt acagggagta cctggtcccc
     3061 ctggagtacg agaggatcat gcccttcccg gggacgcggg aagccttggc aaggctcctg
     3121 gatgaaggct tttccctcgg ggtggtctcg aacaggaaga aactgctccg ggccgtcgaa
     3181 agtgccgggc tcggggagta cttcctctcc ttcgtcggca tggacgatgt ggaaaacccc
     3241 aagccgcacc cggaatcgat atggttatcc atgagtaacc ttgggagcac cgcctcggga
     3301 acgaccctgg tgggcgattc cgagatcgac gccctagcgg cggggagggc cggcatcggg
     3361 tttgtcggcg tgagctcggg aggggccagc cctgatgccc tttcgagtgc gggggccctg
     3421 gtcgtcatca aagacatttc ggaactcgcc tggatcctgc ctgggaggac aggaaggcgc
     3481 cttcagtgcg ggccggggtg ataagataaa agcatgaaag gtaaaaccgc gacgaaggcg
     3541 ttctactatt tcttcaccgt ctgcattgtc cttggcgcga tccttgtcta taaggcctgg
     3601 tgggaccgtt atttccgcct caggcctgaa ctggctgtgg cggaaccagt ggccctctcc
     3661 ctggacatac cgctggaagg cgtgctgatc tgggaagaag tggtgatggc ctcgccagcc
     3721 gagggcccgg tgtcctaccc ccgaggcatg ggacccgcct attgcgcccg tggggacgtg
     3781 gtggctgaag tagcctctcc cgcgggcaga agacaggtga aggcgcccag cccgggttat
     3841 ttcatggcgg ccctcgacgg ggccgagggc ggctggtcct atggagtcct ttggcccggg
     3901 agttcaaggc tccccgaacc ggggccggcc cgtttcctgc acgagggcca gagggtctcc
     3961 ttgggaggag ggatcggtaa attcataccc cagccccaaa ccctgaggtg cgtggcctac
     4021 gtggagaaat cggccctggg ctggaacccg gccgacaggg accttctcga ggtgaaaaga
     4081 accgcctcgg acctttcctt cagggcggaa gtccgcgcgg tgaatgacct ggggcccatt
     4141 tacaaggtct acctcacctt cccgttcttc tcatcggcgg acgtcctctc caggaaggta
     4201 tcttatctgc tccatgccga ggacgtgagg ggggtgctca ttccggaaag cgccgtcacc
     4261 ctccgggaca ggaaaaagat ggcgttcctc gtaaccggtg aaaggtccat tgccgtggag
     4321 gtgacgggta taccagtatc ggggaagcgt ttcctggtta aaaaagggct cgagcccggt
     4381 gacctggtga tagcatcggg agcggatgct gccgaggggg aggtgaggat ctggtgagta
     4441 ccatagcgga caacatagcc tcggtccggg tgaggatcgc cagggcggca acccgctgtg
     4501 gccttgaagg cgacagggtc gacatagtgg ccgtgaccaa gacccgttct atcgatgaga
     4561 tggaagaggc cgcccgctgc gatgtgatcg ccctcggcga gaacagggtc caggaagccc
     4621 tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaccag gatgcttttt
     4741 gcctcaatga gggaactgag ggatgaaacc gaatcacggt taggtctttc gctgccggtc
     4801 ctgtccatgg ggatgagcgg ggactttgag tacgcagtga tggaaggaag caccatggtg
     4861 cggataggga gtgccatttt cggaggcagg ataaactgac ccgcgggcag ggagggaagg
     4921 aattgtcaga cctgttgacc gttcttgata tcgagaggat cagtttcaac aaggccatga
     4981 ggggctataa cccccaagag gtcgaggaat tcctggaaaa agtcgctgaa agcctgcagg
     5041 tttacgctga aaaggtcaag accttggaag tgcagctttc aaggtttgac gagcagatcc
     5101 aggagtacgc cgaactgaag cagtccctgc aggaggccct cgtccttgcc cagaaaagcg
     5161 ccgaggagag ggtgagcagc gccgaaggca aggcggaggc cattatcgcc gaatccgagg
     5221 cccaggctca ccagatcgtc atgagcgcca aggacagggt ggacacccac aagcgggata
     5281 tccagaggtt gaggcaggtc cgttaccagt tcgtggcgga gttcaagggc ctgcttgcca
     5341 agttcgggtc gttgctgaat tcgctggagg cccccgtgga ggaagaggag ggcgaaaagg
     5401 aaggggagag ccatcggtga cggagggcct gacgagagac acccctgtaa ggttcctgaa
     5461 gggggtcggg cccgtaaagg cctctcgact tctcacgctc ggagtgcaga ccgttgagga
     5521 tctcctttac ctttttcccc gtcgttacga gaacagggga gtggccgcgc ccctgggttc
     5581 cctccaggcc ggttccttct cctcggccgt cgccaccgtg ctggccatcg agaggaaacc
     5641 caccaggagg agaaacctca ccctggtgac agccctgctc agcgacggct cgagcctcgc
     5701 ccaggccgtc tggttcaacc ggaaaggcct ggagagggtc ctgacaccgg gtacgaaggc
     5761 ttccttttac gggaaggtcg agagaagagg aggccttttg caatacacca acccggagtt
     5821 cgaggtactg gccgatgagg acgatccctc cgaccaaagg acggtggtcc ccgtttaccc
     5881 cgggacggag ggtttgtacc agaagtggct tcgaagactt atatccgacg ccctcgaggc
     5941 tttccctggt gatctcgtcg accccctgcc tccatctatc cgggaggcca agggtttcat
     6001 gtcccgggtg cctgccgtcg cccagatgca ttacccctcg ggcagggaag aatgggccct
     6061 ggccaggaaa agactcgcct acgaggagct cttcctcctc caggtagccc ttgccatcag
     6121 gaagcagggg taccgggaag aaacgggagg ggaacccctt gagtgggaag gacccttgat
     6181 gaaacatttc cgcgagggca tccttccctt ttcgctgacc cggtcccagg agaaggtcct
     6241 gggcgaactc gctgccgaca gttcgggcaa cgtccccatg aacaggctgc tccaggggga
     6301 cgtgggttcc ggcaagaccg ccgtggcggt gatcttcctg atcgcggcgg tggacagcgg
     6361 cttccagggg gcgatcatgg cccccaccga gatcctggca aggcagcatt tcaagaggat
     6421 atccggttgg ctttccgccc tgggtgttcc cgttcacctt ctcacgggat ccctccccga
     6481 tccgcaaagg gaagggatcc tcgcggatat tgcctccggt gagccctgta tcgtggtggg
     6541 aacccacgcc cttatacaaa aaacggtccg attcggccgt ctggcggccg tggtggtgga
     6601 cgagcagcac cggttcggtg tcctccagag aggggccctg acggctaaag ggagccaccc
     6661 ccacgtgctc gtgatgaccg ccacccccat accgaggacg ctgaccctct ccgtttacgg
     6721 cgacctggcc ttctccgtga tcgatgaact cccccccggg aggaccccga cagagaccag
     6781 gtacctgcgc gaccccgatg acccccggct cgcccggttc attgccaatg agacggccca
     6841 gggaagaagg gtctactggg tctgcccggt gatcgaggag agcgacaagc tcccgctgat
     6901 gcccgtcatc aggcgccacg aaacgctttc aaaacggttc cccggggaga aggtcggcat
     6961 ccttcacggc cagcttcctc ctgaggaaag gcagtccacc atggagaggt ttgtcagcgg
     7021 agagctgtcg atcctcgtat ccacgaccgt cgtcgaggtc ggggtcgacg tgagggacgc
     7081 cacagtcatg gtgatcgagg atgcccatcg tttcggcctg tcgcaacttc accagctcag
     7141 gggcagggtg gggaggggcg aggtgggcgg tttctgcttt cttgtcggca agcccggtac
     7201 ggaggatgga agagcccgga tcgaagcgat gcgcacttct tcggacggct ttgagatagc
     7261 cgaaagggat ctggccctca ggggtccggg tgagatatgt ggggtaaggc agcatggcat
     7321 aaccgatttc aaggtggcgg acctcgtgaa ggacagggac ctcctggagg aggcaaggga
     7381 agatgccttc acgctcgtcg ggtcggaccc ggtattgtcg ggctaccccg accttcggcg
     7441 ggaagttttc aggaaattgg ggacgaagct gaaactggcg gggacggcgt gacctatgaa
     7501 aagggacgat agtttcgaag gcctgcttga atatgccatg aggctgattt cccggtcaga
     7561 agtgacctgc agccaactca agagaaagct cgttgccagg ggggcttcga gccggacttc
     7621 cgagaaagtg tgtcgagaac taaaagagtc gggatacatc gatgaccggc gctactgcga
     7681 gctctttatt tcaacccacg gggaatacgg ctttaaaagg atgaaggtgg agctcctgaa
     7741 aaggggcatt gaacgggatc tcgtggagag tatggtagcc tttgaccagg aagatgaggt
     7801 tgggaaagcc ctggccatga tttgccactg ggggcctgat cttgagatgg agaaggcctg
     7861 ggggaggttg tcccgtaggg ggttctccta ccccgtcata agggaagctc tccggcggac
     7921 ttgtggtgaa gcctcctaat ctgtagaata catcaggacg cttttttgcc tattgcgact
     7981 gccgaataga ttgacaatca atgaaacttg acaacggaat aaggagtgat gtccgaatgt
     8041 tgcctgtaat acttgcagca gctttcgccg tcggtctttc gatcggcttc ctcttttaca
     8101 aaattttgag cgacaggaag ctcaagggga cccgggaaca gtccgaccag cttttgaagg
     8161 atgctttcca ggaagccgac aggcggaaaa gggagatgtt aacggaggcc agggaggaga
     8221 tccaccgcct ccgacaagag gctgaacgcg agatcaagga aaggcgcgcc gaacaacagc
     8281 gcgccgagcg aaggctcgaa caaaaggagg agaacctcga ctccaaaatg gagtccatac
     8341 tccagaggga ggaggagtac cgcaagaagc aggaggaact agcgtcccgc ctcgaagatg
     8401 tcgagtcagt gagaaggaag cagatggaac gactcgagga gatcgccggc atgtcaaggg
     8461 aagatgccaa ggatttcctc ctgaaggaag tggaagaaga ggcaggacac cttattgggt
     8521 taagactcaa ggacctcgag gagaaggcga gaagggagtc cgaaaggaaa tccagagaaa
     8581 ttgtcgtgag tgccatccag aggtgcgccg tggatcatac ctcggaaatg tccgtgagcg
     8641 ttgttacctt gccatcggac gagatgaagg ggcgcataat tggaagggaa ggaagaaaca
     8701 tccgtgcctt tgagacctac acgggagtcg acctcatcgt ggacgataca ccggaagcgg
     8761 tgacgttgag cagttttgac cccgtgagga gagaggtggc gcgtctctcg ctggagaggc
     8821 ttgtcctcga cggaaggatc caccctgcaa ggatcgagga gatcgtggaa aaggcccagc
     8881 gtgacgtgga agaaaagatc ctggaggccg gcgaagaggc ccttcttgaa acagggatca
     8941 aatccatgca cccggaactg gtcaaactga tcggtcaact gagattcagg tccagctacg
     9001 ggcagaatgc gctttcccac agccttgagg tttctcatct ttcggggatc atggccgccg
     9061 aactgggcat agatgaacat gtcgccagga gagccggcct tctgcacgat atcggcaagg
     9121 ccgtcgacca ccaggtcgag gggccccacg cacttatagg tcgtgacctg gccaagaggt
     9181 tcggtgaaac ccccgaggtg gtgaacgctg tggcggctca ccacgaggac caggagatga
     9241 cctccgttta cgacgtgatc gtttcggcgg ccgacgccgt cagcgcctcc aggccggggg
     9301 cgaggcggga gagtctcgaa tcctacatca agaggcttga gacccttgaa acgctcgcca
     9361 agaccttcaa gagtgtgacc aaggcctatg ctatccaggc tggtcgggag gtcagggtca
     9421 tggtcgcgcc gaacagcctc gacgacggct ccatccataa gcttgcctat gatatcgcca
     9481 ggaagataga gagcgaaatg aagtaccccg ggcagatcaa ggtgaccgtg ataagggaga
     9541 cacgctcggt ggaatacgcc aagtaggtga aggtattgaa agtgcttttt ataggggata
     9601 tcgtcggcca ccccggcagg agggcgctgg cgggcctgct cccccgggcg aggaccgaat
     9661 gggggccctt tgatttcgtc gtggccaacg tggagaacgc cgccgggggg ttcggattga
     9721 cggaaaaggt catggaagaa ctgttccgcc agggcctgga cgccatgacg agcggcaacc
     9781 acatctggga caacagggag gccctgcccc ttctgggcgc cgagagtcgg ttggtcaggc
     9841 cggcgaacta cccgccggcc gcgcctggga ccggatcgac ggtgatcgag aaaaaaggtc
     9901 tcaaactggg gctcctgaac ctccagggga gggtcttcat gcaacccatc gattgcccct
     9961 tccggagagc cgacgaagaa ctccttcttt taaaagaatt cccggtactg gtggatatcc
    10021 atgccgaggc gacctcggaa aagcgagccc tggccctgta cctcgacgga agggtttccg
    10081 ccgtgctcgg tacccacacc cacgtccaga ccgccgatga gggcatcctg gagggtggta
    10141 cagcctatat ttccgatgtc gggatgacgg gcgggcacgg cggggtcatc ggtatggaca
    10201 aggaagccat cgtctaccgt ttcctgacgg gtatgcccac gcggttcgag gtctgtaaaa
    10261 gcgacctgag gatggacgcg gtggcgattg agatcgatga agcgtcaggc aggagccttt
    10321 cgataacccg tctttccctg gacctgtgaa gtgttttttg cgagagacct atgaaaaaag
    10381 ggcggggcgc acagcgcccc gccctttttt attgacctcc cggcctcgct ggtataccat
    10441 aaagagcacg cagcatccgg ctgtaacgag ggagatggaa atggaagagt gtcatagtac
    10501 cgggttccaa cgtcacggga ccatgaaaga gagggtgatg gccggggaaa agtagggcac
    10561 cgaccctgac ttggaccttc cctcccggca gagtacaaac cttcccgccg ccggagttca
    10621 ccctcgaaaa ggaaaaactg gacgtgcaga gccaagttcg atagcgtgtg cctcccgtgc
    10681 gcaagacggg atcgccgggc tccctgcatg gccatgtcga tcgggggtgc tcggaccatg
    10741 aaagagagac tggaccgttt ttacaaccag atagaaagcc ttatgtcgga acacaggttc
    10801 accgagatca aggagaccct ttccaccatg gaaccggccg acatagggga gatgctcgtg
    10861 gaagcgcccc aggcagaaag ggtgctcatt ttccgtcttc tttcgaagga tctcgcgatc
    10921 gatgtttttg aacacatgga aggcgcagag agggaggagc tcctcgattg ctttaccgcg
    10981 aaggaagcgg cggagatcat cgaggagatg tccgacgatg accgtaccgc cctgctggac
    11041 gaactgcccg ccaagacggt cgagaagctg atgcagcacc tttcccccga ggagagaaag
    11101 atcgcaaacc acctgttgaa ctacccggag gactcggcag ggaggatcat gaccgcggag
    11161 tatatcggcc tgaagtcatc gatgatggcg acggtggccc tgcagcatat ccggaaggtg
    11221 gcccggagca aggagaccat atacacttgt ttcgtcatgg acggtgagcg ccgcctccgg
    11281 ggggtcgtgg acctggagga tattatcctc gcccagcctg agacgctagt ttctgacctg
    11341 atggatgacg acccggtgtt cgtcacgaca ctgacggacc aggaaaaagt ggcgcaggtc
    11401 atgtcacgtt acgaccttca cgttatcccc gtggtggacc tggagaaccg cctcgtgggg
    11461 atagtgacct tcgacgacgt gctcgacgtc gtcgaggaag aggctaccga ggacttcgag
    11521 cgcatggctg gtatacagcc tttcgaggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnc acgaaccatg agatcagcat attctcggca gcaattcccc gacgggcctg
    11701 tccaccagcc gggtgccgcc cgagtgggtc ctgatgccca cgaggcctgg gtgttccttc
    11761 gtcacacggc cgatgaccga ggccctttcc ccaaggctat gccttttaag caacgtcacc
    11821 gtcctttgag cttccccttc ttccacggct accgccgcgc agccttcccc ggccatgtgc
    11881 aacggctcga acccaaggag gtcgcaaacc gaaagggccg cctcgtccca gggtatcagc
    11941 gattcgtcga tctcgatccc gagcccggcc ctttccgacc actcgcagag caccgtggcg
    12001 aggccgcccc tggtacagtc ccgcatgcag cggagcccgc tcacgggaag gacgctctcc
    12061 agcatgggcc acaaggcggc gcaatctgaa gacaggcccc tggccttcag tccgtagcgg
    12121 tgggccgcga tggtggcgcc gtgcagcccc ggcggacaag tgaggatgac ggcatcgccg
    12181 gggcgcaagt tgacggcccc taacggatct tcccgtacca tcctgccgag gcagcaggtt
    12241 gataggaaca gcccgtcgac ctggcccctg gggaccacct tgctgtcacc cgtcacgagt
    12301 tcgaccccca gggagcggca aacggcggcg gcgctttcca tgtataccaa cagctccctt
    12361 acggggaacc cttcttcggc gatgaccccg agggccaggt agagcggctt tgcgcccctt
    12421 accgacaggt cgttgacgct tccacagacg gagagttttc ctatgtcacc cccctcaaag
    12481 gaaaggggtg acacggtgta tccgtcgatg gtcacagcga accctcctgg gaggatcgcg
    12541 cagtcctctc catcggccaa ggggctgcca tcgaaacggc ccgttatttg gtcgataagt
    12601 tcctgggtga ggctcccgcc tgctccatgc ccgatgctca gtctttccag tccaggtacc
    12661 ccctcttctg gaaacgataa taggccgcgc aactcccctc cgttgagacc atgcaaggtc
    12721 cgacaggatg cgcgggagtg caagatcttc caaagaggtc acaggatggc gggtcgatca
    12781 accctttgag aacctccccg cagcggcacc ccactggggg cggggtctct ttcaccgcca
    12841 ccccgagcct ttccacagcg tcgaagcgag cgaattcctt cctgaggaaa aagcccgagg
    12901 agggaataac tccgagcccc ctccaggggg cgtcgcccag ggtgaacact ctatccatga
    12961 cgttcctggc ggccacattg ccctggggcc tcaccgcccg gctgtaaagc gatgccaccc
    13021 tcggtctttt cattcggatc tgcctcgcca cttccaccag ggccatcatt attccctccg
    13081 cttcgaagcc cgccacggca caggcgatgc cgaatcgttc cggcaggaag cggtaggttg
    13141 cgagccccgt gatgacgctc acgtggccgg ggaggagaaa agcatcgagg ttcacatcgg
    13201 ggtcggaggc gagcgctccc agggccggcg gtaccgattt gtgcaggtcg aggaccatga
    13261 aattgtcgag gccttcccgc caggcgtcca gaactaccga ggccgtggcc ggggccgtcg
    13321 tttcgaaacc gaccgccaag aaaaccacgg aacgggtcct gtcattcatg gcaagcgaaa
    13381 gggcttcgtc ggccgaggtg accactctca catcggcgcc ccgggatctc tcttccgcca
    13441 gggtcgtctc ggttcccgga acccttagca tatccccgta ggtcgctatg gtggtatcct
    13501 cgcggctggc cagggagagg gcggcatcga tctcgccctg ggatgtaacg cagacagggc
    13561 accccggccc cgacacgagg gagatctttg ccggcagcaa ggttctcaac ccctggcgaa
    13621 agatcgagac ggtgtgagtg ccacagactt ccattacctt tataggaggg tcggccagct
    13681 cactcaaagc ttttgaaagg agttctacac gctttctcgg gtcaccgggc ctatgggcct
    13741 gcaagctcac ggatctcctc ccatagtttt tccagttccc ccgtttcctc ctcgaggagt
    13801 ctttcgatgg cgaagcccgc gtggacaagc accatatccc cctccttgat atgaggaacg
    13861 aggtcggtcc ttatctcgac ttccaccgga ccggccaagg ccaaggccct gttaggtcct
    13921 ttcaaggcga tcaccctgtg agggatggcc aggcacatcg ccgctccact cccttccct
//
LOCUS       DAOSRG010000011        13868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4497_length_13868_cov_9.466227, whole genome shotgun sequence.
ACCESSION   DAOSRG010000011 DAOSRG010000000
VERSION     DAOSRG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_13868_cov_9.466227"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLM30_00940"
     CDS             complement(<1..524)
                     /locus_tag="PLM30_00940"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOR53764.1"
                     /translation="MNWTLILILVAVSAIVAYMGDLVGMRVGKKRVSIFGLRPKSTSS
                     IITIFSGVLITILTLAVLTTTSQTVRTAIFSMKFVQRQITDLTSQLQGSRGELEDLET
                     RLMENQEDLMSKQLQLAAVEGRLNESETRLKEIGEELGTTRKEQEKALASLASLQQER
                     DRLDLEVNALRAES"
     gene            complement(669..2033)
                     /gene="lat"
                     /locus_tag="PLM30_00945"
     CDS             complement(669..2033)
                     /gene="lat"
                     /locus_tag="PLM30_00945"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HOR53765.1"
                     /translation="MAKVDRRVEPGQVFQEISKWLLRDGFDIVVDMEKSKGSHMINKV
                     NGEDWLDFYTFFASAPFGMNHPKLDNPEFKEIIFRAAINKVASSDIYTDQMAQFVKTF
                     GDVAVPKGFNHVFFIDYGTLAVENTFKVAMDWKVQKLLQQGKCCRGDAVKGRRGTKVI
                     HFNDAFHGRSGYTLTVTNTQDPNKYKHFAQFDWPRVLNPKIYWPLEDNLGVVTWLERV
                     AIKQIKEAIWDNPDDICAIIIETIQGEGGDNHFRTEFFQQLREICDEEDILLIFDEVQ
                     CGMGITGKMWAFEHHAPVKPDMFAFGKKTQICGLVAGPRLDEVENNCFKISSRINSTW
                     GGTVVDMVRATRYLEIYEEDNILDYVANTAGPALLGGLKKLQEEFPGLISNARGKGLM
                     CAYDFKTTAMRDKALKLFWAKKMLVLPCGDVSIRFRPALNVPVADLEAGLSIAREVFR
                     ECSK"
     gene            complement(2178..2657)
                     /locus_tag="PLM30_00950"
     CDS             complement(2178..2657)
                     /locus_tag="PLM30_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOR53766.1"
                     /translation="MISRSGPLFGVEKGGALELPYEPWWQEGLFFSCIGCGRCCRGEP
                     GSVWLSPGELEAVAEYLGMESEAFRKGFVVERRGRMSLRERPNWDCVMYQGESARCGI
                     YSVRPLQCRLFPFWPSILRTRRGWVERSSECPGMDRGRFWSAEEIAALLAKSPFPDL"
     gene            complement(2659..3702)
                     /locus_tag="PLM30_00955"
     CDS             complement(2659..3702)
                     /locus_tag="PLM30_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOR53767.1"
                     /translation="MWGMDIGIDLGTANVLVFMKNNGVVLREPSVVAVDLNTDRILAV
                     GNEAKNMLGRTPGNVVAIRPLKDGVIADYTMTEAMLRHFLKKVIKGFGRLMRRRAMIC
                     VPSGATDVERRAVLEAAVEIGTKEAFLIEEPMAAAIGANLDISEPRGNLIVDIGGGTT
                     DVAVLSYGGIVVAESLRLGGDRFDENITRYIRKEYNLAIGEQTAEAIKINVANCAPSP
                     RQEQTMMIKGRDLVHGLPRQIEITDRIVAKAIEETVGMIIEGIRQVLERTPPELSADI
                     IDRGITLSGGGALLKGLPELVTKKTQIPAYVADMPLECVAIGTGKALDDLDKLRSSGS
                     LIGASRRRERKVW"
     gene            complement(3801..4562)
                     /gene="truA"
                     /locus_tag="PLM30_00960"
     CDS             complement(3801..4562)
                     /gene="truA"
                     /locus_tag="PLM30_00960"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR53768.1"
                     /translation="MRFAAKIAYDGSRFSGWQKQPKATENTVQEHVERALATLNGRST
                     PVVAAGRTDKGVHALGQVISFDMAAPWEPRKLRNALDANLPRSISVSRVAPVPDDFSA
                     RRSADWREYVYFVWKGQGCPPHILPFVWRNSSDWDRGTLRTLCALMKGEHDFSAFCRK
                     LDRPADTRRTILAASVSGKGPLLRVRIRGDSFLTNMIRIIMGSMDLVAAGKRDVPWFE
                     GLLEGGQRVDAGPTAPPDGLFLTLVGYTPSPWDVS"
     gene            complement(4559..5374)
                     /locus_tag="PLM30_00965"
     CDS             complement(4559..5374)
                     /locus_tag="PLM30_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOR53769.1"
                     /translation="MKFLNNMTLGQYVPVESPVHHLDPRCKIVAVLFCLVGIFMVRGP
                     LGFVMWGLFFLALVGASRIPARLVLSTVKPVWILVAFTAAIHLFFTGGEPVFSWGILE
                     VTREGVVMAARMGLRLMLLIMFAGLLTLTTSPTELADGLESLFSPLRRFGFPAHELAM
                     MMTIALRFIPTLLNETERIMKAQIARGAELERGGPLKRLRAFIPVLVPLFVIVFQRAD
                     DLATAMEARNYTGGEGRTRMNPLEWTAGDSAAFVFSLLFSFSAVFLDGRVTLP"
     gene            complement(5371..6213)
                     /locus_tag="PLM30_00970"
     CDS             complement(5371..6213)
                     /locus_tag="PLM30_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR53770.1"
                     /translation="MSIAVRDLVHIYHPGSPLQTKALDGVTFEAERGSWVAVVGHTGS
                     GKSTLAQHLNGLLLPTEGSVSVDGIQVRHGEESLRKVRQKVGLVFQYPEQQLFEETVF
                     KEVSFGPRNWGFQAGRLEKSVEEALELVGIGEDLYEANPFRLSGGQKRRVAIASTLSS
                     DPDYLVMDEPTAGLDSPGRRQLLGLLSGLKKLGKGIIHITHDMELALGLADTILVLDE
                     GKSTLWGPPGVILGELLERDIRGLVIPDLVRFAGRLRDLGFEVPLTWEPKILAEAIGR
                     SHGR"
     gene            complement(6204..7031)
                     /locus_tag="PLM30_00975"
     CDS             complement(6204..7031)
                     /locus_tag="PLM30_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR53771.1"
                     /translation="MTADCLVRFSGVSFTYQGTDAPAIADIDLKIGAGEWIAVLGSNG
                     SGKSTLAKHFNALLLPTQGDCFVHDLNTKTEEGKRSARRSVSMVFQNPENQLIAAVVE
                     EDVAFGPENLGLSPGEIRERVRWALDVSGLGNMARKPVYALSGGQKQRLALAGAIAQR
                     TLCLVMDEATTMLDPKGRQDLMAVLSSLHTGGMTLVSITHRLEEILCCDRCIVLYGGR
                     VAWEGTPEALFLLGEELVRWGLEVPGMVKVWRMLLEEGLIRKGTPPRAEEMVEALCR"
     gene            complement(7028..7387)
                     /gene="rplQ"
                     /locus_tag="PLM30_00980"
     CDS             complement(7028..7387)
                     /gene="rplQ"
                     /locus_tag="PLM30_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOR53772.1"
                     /translation="MRHRVDRRRLGRYGSHRMAMLSNLTADLFLEERLETTVTRAKEV
                     RRVAERMITKAKAGDLHNRRIVASRMGNVKAVQKLFNDIAGRFSERQGGYTRITRLGY
                     RKGDGAPMAVIELVERS"
     gene            complement(7384..8394)
                     /locus_tag="PLM30_00985"
     CDS             complement(7384..8394)
                     /locus_tag="PLM30_00985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR53773.1"
                     /translation="MTGRKMMEHIKPEIRVEESTPLNGRIVVSPLEKGYGVTLGNALR
                     RVLLSSIGGAAITAVRIEGILHEFSTIPGMREDVIELLLNLKNVALKSYSEGTKILRL
                     EAKGPCVVTPVHIQPDSDIEFVDLDRYICTLEEGGFLEMDLFIEKGTGYLTSERDRPS
                     DLPLDALVMDAVFSPVRRAKYEVQDARVGQRTDYERLVLEVWTDGSIMPEKAVIDAAS
                     ILQRSFSLFVKGLGKDESEGAPDGEGDGSTGKDKDTDGVFARSIKDLELSIRSENCLL
                     RGGVQTIGDLMSRGRSELLKIRNLGKISLREIEEKLEAFGLQISEDDRPSEDKSKEDE
                     AR"
     gene            complement(8398..9027)
                     /gene="rpsD"
                     /locus_tag="PLM30_00990"
     CDS             complement(8398..9027)
                     /gene="rpsD"
                     /locus_tag="PLM30_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016801712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR53774.1"
                     /translation="MSRYTGAVCRLCRAEGTKLFLKGDRCYTEKCAINKRNYKPGQHG
                     QSRRIKVSEYGLRLREKQKLRRFYGINESQFRRFYAAASRMAGQTGHNFLRLLEARLD
                     NVIYRFGIGVSRPQARQFVRHGHVTVNGRKVDIPSYLVKAGDVIAIGERSRDKALLKE
                     NIEVAASRTIPEWLQFDAEKQEGRILALPNREQIEVPVKEQLVVEFYAR"
     gene            complement(9054..9446)
                     /gene="rpsK"
                     /locus_tag="PLM30_00995"
     CDS             complement(9054..9446)
                     /gene="rpsK"
                     /locus_tag="PLM30_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR53775.1"
                     /translation="MAKRTQRRGKKKEKKNISYGVAHIYSTFNNTIISVTDRQGNLLS
                     WASGGHVGFKGARKSTPFAAQLAAQQVAKQAQDHGVQEIDVVVKGPGPGRESAIRSLQ
                     GAGLQVNAIKDETPIPHNGCRPPKRRRV"
     gene            complement(9466..9849)
                     /gene="rpsM"
                     /locus_tag="PLM30_01000"
     CDS             complement(9466..9849)
                     /gene="rpsM"
                     /locus_tag="PLM30_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR53776.1"
                     /translation="MARIAGVDLPREKRIEVALTYIYGIGQPSSKKILEATSIDPDRR
                     VKDLTEEEAQKLRSEIEENYRVEGDLRREVTMNIKRLIEIGCYRGMRHKFGLPVRGQR
                     TKTNARTRKGPRRAVAGKKKPAAKK"
     gene            complement(9856..9981)
                     /gene="rpmJ"
                     /locus_tag="PLM30_01005"
     CDS             complement(9856..9981)
                     /gene="rpmJ"
                     /locus_tag="PLM30_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOR53777.1"
                     /translation="MKVKPSIKPICEHCRVIKRKGVVRIICSKNPRHKQRQGARR"
     gene            complement(10057..10275)
                     /gene="infA"
                     /locus_tag="PLM30_01010"
     CDS             complement(10057..10275)
                     /gene="infA"
                     /locus_tag="PLM30_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR53778.1"
                     /translation="MPKDDMIEVKGKVIEPLPNAMFRVELENGHKILGHVSGKMRMHF
                     IRILPGDRVLVQISPYDLTRGRIVYRYK"
     gene            complement(10377..11147)
                     /gene="map"
                     /locus_tag="PLM30_01015"
     CDS             complement(10377..11147)
                     /gene="map"
                     /locus_tag="PLM30_01015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR53779.1"
                     /translation="MVTIKNEQEIASLRKAGKIVADVLHLVRDIIRPGVDTLTLDAEA
                     EKLIIASGARPAFKGYTVPGIPVPFPGTLCVSINDEVVHGKPDRDRILQEGDVVSVDV
                     GVRFEGFYGDAACTFPAGDISPEREALLRVTRECLERAIAASREGATLGDIGHAVESW
                     AVPRGYGVVREYAGHGIGRKLHEAPQIPNYGVPGKGITLKRGMSLAIEPMIMTGKEAV
                     RTLKDGWTVVTADGSDAAHFEKTIVITGGDPEVITPWD"
     gene            complement(11151..11795)
                     /locus_tag="PLM30_01020"
     CDS             complement(11151..11795)
                     /locus_tag="PLM30_01020"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOR53780.1"
                     /translation="MRVIFLGSPGAGKGTQAAVLKDRYCLAHISTGDILRENVRSGTA
                     LGKTAKKYMDSGGLVPDDVIIAMMKERLQEPDCRGGFILDGFPRTVPQAEALDRLLEG
                     MGITLDGVILFEIPRQLVIDRLTGRRVCGSCGEIYNVRFKNTKTPGVCDRCGGELTQR
                     DDDREEVVIRRLEIYEEQTAPLVRYYEEKGRLIRVDAGQEGDRVVAAIEKAVGL"
     gene            complement(11812..13107)
                     /gene="secY"
                     /locus_tag="PLM30_01025"
     CDS             complement(11812..13107)
                     /gene="secY"
                     /locus_tag="PLM30_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR53781.1"
                     /translation="MLDAFRDAFRLPDLKRKILFTMGVLFLFRLGAHIPTPGIDPDAM
                     ASLFDQGGVLGFLDLFAGGALRRFSVFALGVAPYINASIVMQLLVVVFPALEKLQKEG
                     EQGRKKIVQYTRLGTVFFAAVQAIGMTFWLRNLGVFSGGWVGFVAAIFTVTAGSLVVM
                     WLGEEISDHGIGNGISLLIFAGIVARLPEAMIRTWSLLRMGEMNILVISLALILILVV
                     IAGCVLLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQAGVIPIIFASSVLLFPS
                     TIARLFSGEVSAVIQRFLSPDSLVYMTLYVVLIVFFSYFYTAVVFDPNDTAENMKKYG
                     GFVLGIRPGKPTAEYIEKVMSRITLGGAVFLAVVALLPTLMTRLMGITTFYFGGTAVL
                     IVVGVALDTVHQVEAQLLMRHYEGILKRSKGKTAGLLKF"
     gene            complement(13108..13554)
                     /gene="rplO"
                     /locus_tag="PLM30_01030"
     CDS             complement(13108..13554)
                     /gene="rplO"
                     /locus_tag="PLM30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR53782.1"
                     /translation="MGIHELRPAPGSRRKPKRMGQGTGSGTGRTAGKGTKGQKARSGG
                     GVRPGFEGGQMPLTRRVPKRGFSNARFKKTFQLVNVGRLGDHFEDSGEVGIPEMLMAG
                     LIGDAKRPVKILGDGEVTRALTVKANAFSAQAVAKIEAAGGKTEVI"
     gene            complement(13569..13754)
                     /gene="rpmD"
                     /locus_tag="PLM30_01035"
     CDS             complement(13569..13754)
                     /gene="rpmD"
                     /locus_tag="PLM30_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOR53783.1"
                     /translation="MAKLKVTLRKSTIGRPPRQGETVRALGLRNIRQTVIHEDTPQVR
                     GMIAAVEHLVDWEIVEE"
BASE COUNT         2429 a         4484 c         3996 g         2959 t
ORIGIN      
        1 tccgattcgg ccctgagggc gttcacctcc aggtcgagcc gatccctctc ctgctgcagc
       61 gaggccaggc tcgccagggc cttctcttgc tctttcctgg tcgtaccgag ttcctcgcct
      121 atctccttga gacgggtctc gctctcgttg agccttcctt cgacggcggc cagttggagc
      181 tgcttgctca tgaggtcctc ctggttctcc atcagcctgg tctcgaggtc ctcgagttca
      241 cccctgctcc cctggagttg ggaggtaagg tcggtgatct ggcgctggac gaacttcatg
      301 ctgaagatgg ccgtcctcac ggtctgggaa gtggtggtca ggacggcgag ggtgagtatc
      361 gtgatgagaa ccccggaaaa gatggtgatg atcgaggagg tggacttggg cctgaggccg
      421 aaaatgctga ccctcttctt gcccaccctc atccccacga gatcgcccat gtaggccaca
      481 atggcgctca ccgccaccag gataaggatc agggtccagt tcatctctgc aaggagatcc
      541 aacatcacca ccaccccgct ttcaggataa agttaccaca tgagagccct attttcacga
      601 aaaaggccgg agggacaccc ctccggcctc aaagtcaagt tgattccggc accggcgtga
      661 cggggccgtt atttggagca ctccctgaag acttccctgg ctatcgagag accggcctcg
      721 aggtccgcca cggggacatt gagggccggg cggaaccgga tggagacatc gccgcagggg
      781 aggaccagca tcttcttggc ccagaagagt ttcagggcct tgtcccgcat agcggtggtc
      841 ttgaaatcgt aggcgcacat caggcccttg cccctggcgt tggagatcag cccggggaac
      901 tcctcctgca gcttcttcag accgcccaaa agggccgggc ctgccgtgtt cgcgacataa
      961 tcgaggatgt tatcctcctc gtagatctcg aggtacctgg tggccctgac catgtcgacc
     1021 accgtcccgc cccaggtgga gttgatgcgg ctggagatct tgaagcagtt gttctccacc
     1081 tcgtccagcc tcgggccggc cacgagaccg catatctggg ttttcttgcc gaaggcgaac
     1141 atgtcgggct tcaccggggc gtgatgctcg aaggcccaca tcttgccggt gatccccatg
     1201 ccgcactgga cttcgtcgaa aatgaggagg atatcctctt cgtcgcagat ctccctgagc
     1261 tgctggaaga actccgtacg gaagtggttg tccccgccct ctccctggat ggtctcgatg
     1321 ataatggcgc agatgtcgtc ggggttgtcc cagatggcct ccttgatctg cttgatggcc
     1381 accctttcga gccaggtcac cacgccgagg ttatcctcga ggggccagta gatcttgggg
     1441 ttcagcaccc tgggccagtc gaactgggcg aaatgcttgt acttgttggg gtcctgggtg
     1501 ttggtcacgg tgagggtgta gccgctgcgg ccgtgaaagg cgtcgttgaa gtggatgacc
     1561 ttggtccccc gcctcccctt gacggcgtcc ccccggcagc acttgccctg ctggagcagc
     1621 ttctggacct tccagtccat ggcgaccttg aaggtgttct ccaccgcgag ggtcccgtag
     1681 tcgatgaaga aaacgtggtt gaaccccttg ggtacggcca catccccgaa ggtcttgacg
     1741 aactgggcca tctggtcggt gtagatgtcg gaactggcca ccttgttgat ggccgccctg
     1801 aagatgatct ccttgaattc cgggttgtcc agtttggggt ggttcattcc gaagggggcc
     1861 gaggcgaaga aggtgtaaaa gtccagccaa tcctcgccgt tgaccttgtt gatcatgtgg
     1921 gagcccttcg acttctccat gtcgacgacg atatcgaaac cgtcccttaa aagccactta
     1981 ctgatctctt ggaaaacctg tccaggttcc actcttctgt ctacctttgc cattcgagct
     2041 acctcccttt tatttgtctt gggatgcccg ccaggtatcc cacttggcac ccggagcgga
     2101 ggggcctgtt tcttgtttat tatttcacaa ttattcgagc ggggcaacca ggcatcgacc
     2161 cggccggggg tcacccgtta aagatccggg aaaggcgact ttgccaaaag ggcggctatc
     2221 tcctcggcgc tccagaaacg cccccggtcc atgcccgggc attcactgga tcgctccacc
     2281 caaccccgtc tcgtcctgag tatgctgggc cagaagggga agagcctgca ttgaaggggt
     2341 ctcacgctgt atatcccgca ccgggcgctc tcaccctggt acatgacgca atcccaattc
     2401 gggcgttccc tgagggacat cctgccccgg cgctcgacca caaaaccctt ccggaaggct
     2461 tcactctcca tgcctaagta ttcagcgacc gcttccaact ccccggggga aagccagacc
     2521 gacccgggtt caccgcggca gcaacggccg caaccgatgc aggaaaaaaa taaaccttct
     2581 tgccaccacg gttcatatgg taactcaagg gctccccctt tttcaacccc gaaaagcggg
     2641 cccgacctcg atatcatatc accatacctt tctctccctg cgccgtgaag ccccgataag
     2701 tgaaccgctg gagcgcaact tgtcaagatc atcgagggcc ttgcccgtcc cgatggccac
     2761 gcattccagg ggcatgtccg ccacgtaggc gggtatctgg gtcttcttgg taacaagctc
     2821 ggggagcccc ttcaggaggg ctcctccgcc ggagagggtt atgccccggt caattatgtc
     2881 ggccgacagt tcgggggggg tcctctccag gacctgcctt atcccctcga ttatcatgcc
     2941 gaccgtctcc tcgatggcct tggccacgat gcggtcggtg atctcgatct gcctcggtag
     3001 cccgtggacg aggtcccgcc cctttatcat catagtctgt tcctgccgtg gcgagggggc
     3061 gcagttggcc acattgatct tgatcgcctc cgccgtctgc tctcctatgg cgaggttgta
     3121 ttccttccgg atgtaacggg ttatgttctc gtcgaagcgg tcccccccca gcctcagaga
     3181 ctcggccacg acgatgccgc cgtaggacag gaccgccacg tcggtcgtcc cgccgccgat
     3241 atcaacgatg aggttgcccc tgggttcgga gatatcgagg ttagccccta tggccgcagc
     3301 catgggttct tctatcagga aggcctcctt cgtgcctatc tcgacggcag cctccaggac
     3361 ggccctccgt tccacgtcgg tggccccgga ggggacgcat atcattgccc tgcgcctcat
     3421 gagcctgccg aaacccttga tgaccttctt caggaagtgc ctgagcatgg cctcggtcat
     3481 ggtgtagtcg gcgatgacgc cgtccttgag gggcctgatg gccactacgt tgccgggagt
     3541 acgcccgagc atgtttttgg cttcgttacc cacggcgagg atcctgtcgg tgttcaggtc
     3601 cacagccacc acggagggtt ccctcaggac cacgccgtta tttttcatga aaacgaggac
     3661 gttggccgtc cccaggtcga tcccgatatc cataccccac atacaaggtt acctccttca
     3721 agggtcgacg aaaaagaccg ccccgggtcc tcgtggcttc cgcgcccgcg gttagactat
     3781 atccgaaaag gccgccgccc tcaagaaaca tcccatggcg aaggggtgta ccccaccagg
     3841 gtgaggaaaa gcccatccgg cggggccgtg gggcccgcgt cgaccctttg tcccccttcg
     3901 agaagccctt cgaaccacgg gacatcccgt ttaccggcag cgacgaggtc catcgacccc
     3961 atgatgatcc tgatcatgtt cgtcaggaag ctgtcacccc tgatccttac ccgcaaaaga
     4021 gggcccttgc cagagaccga cgcggccagg atcgtccgcc gggtatcggc ggggcggtcg
     4081 agtttgcggc agaaagcgct gaaatcgtgc tcccccttca taagggcgca aagcgtcctc
     4141 agggtccccc tgtcccagtc agagctgttc ctccagacga aggggaggat atggggcggg
     4201 cacccctgtc ccttccatac gaaatatacg tactccctcc agtcggcgct ccgcctggcg
     4261 ctgaaatcgt cggggacggg ggccacccgc gacacgctga tggatctcgg gaggttggcg
     4321 tccagggcgt tccggagctt tcgaggttcc cagggggcgg ccatatcgaa ggatattacc
     4381 tgccccaggg cgtgaacgcc cttgtcggtc ctgccggcgg ccacgacggg cgtactccgc
     4441 ccgttcagcg tggccaaggc cctttcgacg tgctcctgca cggtattttc cgtagccttc
     4501 ggctgcttct gccagcccga gaagcgggag ccgtcatagg cgatcttcgc ggcgaacctc
     4561 atgggagggt caccctgcca tcgaggaaca ccgccgaaaa agagaacagg agcgagaaga
     4621 cgaaagcggc ggagtccccg gcggtccatt cgagggggtt catccgggtc ctgccctccc
     4681 cgccggtgta gttcctcgct tccatggcgg tagccaggtc gtcggccctc tggaagacga
     4741 tcacgaaaag gggcaccagg acggggatga aggcccgaag cctcttcagg gggccacccc
     4801 tttccagttc ggcccccctg gctatctggg ctttcattat cctctccgtc tcgttgagaa
     4861 gggtgggtat gaacctcagg gctatggtca tcatcatggc cagttcatgg gcggggaagc
     4921 cgaacctgcg aaggggggag aaaaggctct ccaggccatc ggccaattcc gtggggctcg
     4981 ttgtgagcgt aaggagcccc gcgaacatga tgagcagcat cagcctcaga cccatcctcg
     5041 cggccatgac cacgccctcc cgggtcacct ccaggatccc ccaggagaag accggctccc
     5101 cgccggtgaa aaaaaggtgg atcgcggcgg tgaaggctac cagtatccag accggtttca
     5161 ccgtggacag gaccagccgc gcgggtattc tcgacgcccc gacgagggcc aggaaaaaga
     5221 ggccccacat cacgaagccc agggggccgc ggaccatgaa gatgcccacg aggcaaaaga
     5281 ggacggccac gatcttgcac cgcgggtcga ggtggtggac cggggattcg acggggacat
     5341 actggccgag ggtcatgttg ttcaggaatt tcatcttccg tggctcctgc ctatcgcttc
     5401 cgccaggatc ttcggctccc aggtcagggg cacctcgaag cccaggtcgc ggaggcggcc
     5461 ggcgaaccgg acgaggtccg ggatgacgag cccccgtatg tcccgttcga ggagttcacc
     5521 cagtatcacg cccggagggc cccagagggt gctcttgccc tcatccagga ccagtatcgt
     5581 atcggcgaga cccagggcca gttccatatc gtgggtgata tggattatcc ccttgcccag
     5641 ttttttcagg ccggaaagaa ggccgagcag ctgcctcctc cctggggagt cgagcccggc
     5701 ggtgggctcg tccatgacga gataatcggg gtcggaggaa agggtcgaag ctatggcgac
     5761 cctcctcttc tgcccgcccg acagccggaa tgggttggct tcgtacaggt cttcacctat
     5821 gcccaccagt tcgagggcct cttcgacgct tttctcaagg cgaccggcct ggaaacccca
     5881 gttcctgggg ccgaaggata cttccttgaa gacggtctcc tcgaaaagct gctgctccgg
     5941 gtactggaag accaggccca ccttctgcct gaccttgcgg agggactctt cgccgtgccg
     6001 tacctgtatg ccgtcgacgc tgaccgagcc ctccgtaggc aggaggagcc cgttcaggtg
     6061 ctgggccaag gtggacttgc cgctgccggt atgccccaca acggcgaccc agctacccct
     6121 ttcggcctcg aaggtgacgc cgtcgagggc cttcgtctgg aggggcgacc cggggtgata
     6181 gatatggacc aggtccctta cggctatcga catagggcct ccaccatctc ttccgcccga
     6241 gggggggtcc ccttgcggat cagcccctcc tcaagaagca ttcgccagac tttcaccatc
     6301 cccggcactt caagtcccca cctcaccagt tcctctccga ggaggaacaa ggcctccggg
     6361 gtcccttccc aggctacgcg gcccccgtag agtacgatgc agcggtcaca acaaaggatc
     6421 tcctcgagcc tgtgggtgat ggaaacgagg gtcatgccgc cggtatgaag gctggagagt
     6481 accgccatga gatcctgcct ccccttgggg tctagcatgg ttgtcgcttc gtccatgacg
     6541 aggcaaaggg ttctctgggc aatggccccg gccagggcga gtctctgctt ctgtccccca
     6601 gaaagggcgt agacaggttt acgggccata ttcccgaggc ccgagacgtc cagggcccat
     6661 ctcacccttt ccctgatctc cccgggggac aggcctaggt tctcagggcc gaaggcgacg
     6721 tcttcctcta cgacggccgc tatgagctgg ttctccgggt tctggaaaac catagacacc
     6781 gaccttcggg ccgagcgttt gccttcttcg gtctttgtgt tgaggtcgtg gacaaaacaa
     6841 tcgccctgcg taggtaacag cagggcgttg aagtgcttcg ccagggtgga tttacccgac
     6901 ccgttgctcc caagtacggc tatccactcc ccggcgccga tcttcaggtc aatatccgcg
     6961 atggctggag cgtctgtacc ctggtaggtg aaagatacac cggaaaaccg taccaggcag
     7021 tcggccgtca tgacctctcg actagttcta tcacggccat gggggccccg tcgcccttgc
     7081 ggtaacccag cctggttatc cgggtgtaac ccccttgcct ttcgctgaaa cggccggcta
     7141 tatcgttgaa cagtttctgc acggccttga cgttacccat cctggaagcg acgatcctcc
     7201 ggttgtgaag gtcgccggcc ttggcctttg tgatcatcct ctctgctacc cggcgaacct
     7261 ccttggccct ggtgacggtg gtctccaggc gctcctccag gaaaaggtcc gccgtgaggt
     7321 ttgagagcat ggccatgcga tggcttccgt accgcccaag cctgcgtcga tcaacgcggt
     7381 gcctcatcgg gcttcatcct ccttcgactt gtcctctgac gggcggtcat cctccgagat
     7441 ctgcagcccg aaggcttcca gcttttcctc tatctccctg agggatatct tgcccaaatt
     7501 cctgatcttc agcagttccg acctgcccct gctcatgagg tccccgatgg tctggacacc
     7561 cccgcgaaga aggcagttct cgctccttat ggataactcc aggtccttga tggaccttgc
     7621 gaagacgccg tcggtatcct tgtctttacc agtcgacccg tcgccttccc catcgggagc
     7681 cccttcggac tcatccttgc cgagcccttt cacgaagaga gaaaaggatc tctgcaggat
     7741 agaggcggcg tcgatcacgg ccttttcggg catgatcgac ccgtcggtcc acacttcgag
     7801 gaccaacctc tcgtagtcgg tcctctggcc gaccctggcg tcctggactt cgtacttggc
     7861 ccgacggacg ggggagaaca ccgcatccat gacgagggcg tccagcggga ggtccgaggg
     7921 cctgtccctt tcggaggtca ggtagccggt ccccttttcg atgaagaggt ccatctccag
     7981 gaaaccgcct tcctccaggg tgcagatgta cctgtcgagg tccacgaact cgatatcgct
     8041 gtcgggctgg atatggaccg gcgtgaccac gcagggaccc ttcgcctcca gcctcagtat
     8101 cttggtcccc tcgctgtaac tcttcagcgc cacgttcttc aggttgagga gcagttcgat
     8161 gacatcctcc ctcatgcccg ggatggtgct gaactcgtga aggataccct cgatcctcac
     8221 cgcagtgatg gccgccccgc cgatggaaga cagcaggacc cgcctgaggg cgttcccaag
     8281 ggtgacgccg tacccctttt ccagggggct gacgacgatc cttccgttca ggggagtgct
     8341 ttcctctacc cggatctcgg gtttgatatg ttccatcatt ttcctcccgg tcatgcccta
     8401 tcgggcgtag aactcaacaa ccagctgttc cttgacgggg acctcgatct gttcacggtt
     8461 agggagggcc agtatgcggc cttcctgctt ctcggcgtcg aactgcagcc actcgggtat
     8521 cgtccgggaa gccgccacct cgatgttctc cttgaggagg gccttgtccc ggctcctctc
     8581 gccgatggcg atgacgtccc ccgccttcac gaggtaactg gggatatcca ccttgcgtcc
     8641 gttcacggtt acgtggccgt gtctcacgaa ctgcctcgcc tgggggcggc tcaccccgat
     8701 gccgaaccgg tagatcacgt tgtcgaggcg cgcctcgagc aaccgcagga agttatggcc
     8761 cgtctgaccc gccatcctcg aggcggcggc gtagaacctc ctgaactggg actcgttgat
     8821 cccgtagaag cgtctcagtt tctgcttctc ccgcagccgg aggccgtact cgctgacctt
     8881 tatccttcgg ctctgtccgt gctgccccgg tttgtagttt ctcttgttga tcgcgcactt
     8941 ttccgtgtaa cacctgtcgc ccttcaggaa gagctttgta ccctcggccc tgcagaggcg
     9001 acaaacggca cctgtgtacc tgctcattac tcctgtcctc cttgatcagc ccgttagact
     9061 cgccgacgct tgggaggccg gcaaccgttg tgagggatgg gggtctcgtc ctttatggcg
     9121 ttcacctgca gacccgcccc ctgaagggac cggatggccg actcgcggcc gggcccgggc
     9181 cccttgacaa cgacatctat ctcctggaca ccatgatcct gcgcctgctt ggcaacctgc
     9241 tgggcagcca gctgggccgc gaagggggtg gatttcctcg cccccttgaa acccacatgc
     9301 ccgccggagg cccatgacag caggttcccc tgcctgtcgg tgacactgat tatggtgttg
     9361 ttgaaggtcg agtagatatg ggctacaccg taactgatat tctttttttc ctttttcttg
     9421 cctcttcgtt gcgttctctt cgccacgcga atactccctc ccttgctact tcttggccgc
     9481 gggtttcttc ttgcccgcga cggccctgcg gggtcccttg cgggtcctcg cgttggtctt
     9541 tgtcctttgc cccctcacgg gaaggccgaa tttatggcgc atgccccggt aacagccgat
     9601 ctcgatgagg cgcttgatgt tcatcgtgac ctcgcgccgg aggtcgcctt cgaccctgta
     9661 gttctcctct atctccgacc tgagcttctg ggcttcctct tcagtgaggt ccttcaccct
     9721 cctgtcgggg tcgatggaag tcgcttccag tatctttttc gaggagggct gcccgatccc
     9781 atagatatag gtcagcgcga cctcgatgcg tttttccctt gggagatcga ctcctgcgat
     9841 tcgtgccatg gggtactacc tcctcgctcc ctgtctctgt ttatgcctcg ggttcttgct
     9901 gcatatgatc cttacgacac cttttctctt gataaccctg caatgttcgc agatgggttt
     9961 gatagaaggc ttaactttca tttgatccca cactcctcaa cgcaactgaa ttacgcgcct
    10021 gccggggaaa accccggcga tcctccaggt tccaagctat ttatatctat acacgatcct
    10081 tccccgtgtc aagtcgtagg gggatatctg gacgaggacc ctgtctcccg gcagtatcct
    10141 tatgaaatgc atccgcatct tgcccgacac gtggcccagg atcttgtgac cgttttccag
    10201 ttcgacccgg aacatggcgt tgggtaaagg ttctatgacc tttcccttga cctcgatcat
    10261 gtcatctttt ggcatatggc tccttcaccc tccctgtccc tctcggcggg acggcccgac
    10321 ccgttcaacc aggccggggc ggccaccggc cccggggaga tcagcgccct gaccctttaa
    10381 tcccaaggcg tgatcacctc cgggtcgccg ccggttatga cgatggtctt ctcgaagtgg
    10441 gcggcatcgg agccatcggc cgtcaccacg gtccatccgt ccttcagagt ccttaccgcc
    10501 tccttcccgg tcatgatcat cggttcgatg gccagggaca tgcccctctt aagggttatg
    10561 cccttgccgg gcacaccgta gttagggatc tgcggggcct cgtggagctt ccgcccgatc
    10621 ccgtggccgg cgtattccct cacaaccccg tagcccctgg gtacggccca cgattccacc
    10681 gcgtggccga tatcccccag cgtggccccc tcccgggagg cggcgatggc cctctccagg
    10741 cactcgcggg tcaccctgag tagagcctct ctttccggcg atatatcgcc cgccgggaag
    10801 gtgcaggccg cgtctccgta gaacccttcg aaacggaccc ccacgtcgac gctgaccaca
    10861 tcgccctcct ggaggatcct gtccctgtcg ggcttcccgt ggaccacctc gtcgttgatg
    10921 gagacgcaaa gggtccccgg gaaggggaca gggatcccgg ggacggtata ccccttgaag
    10981 gccggtcgtg cgccggaggc gattataagc ttttccgcct cggcgtccag ggtaagggtg
    11041 tccacccccg gccggatgat atccctcacc aggtgaagga catcggccac tatcttcccg
    11101 gccttcctga ggctcgcgat ctcctgttcg ttctttatcg tgaccatatc ctatagccct
    11161 acggcctttt ctatggccgc gaccacgcgg tcgccctcct ggcccgcatc gacccggatg
    11221 agtcggccct tttcctcgta gtagcggacc aggggagccg tctgttcctc gtagatctcg
    11281 agcctcctga tgacgacctc ttccctgtcg tcatctcttt gggtcaactc cccgccgcag
    11341 cggtcgcaga ccccgggggt cttggtgttc ttgaaacgca cgttgtatat ctcgccgcag
    11401 ctcccacaga ccctcctgcc ggtcagccgg tcgatgacca gctgccttgg gatctcgaaa
    11461 aggatgaccc cgtcgagggt gatgcccatc ccctccagga gacggtccag ggcctcggcc
    11521 tgggggaccg tcctggggaa cccgtccagg atgaagccgc ctcggcaatc gggctcctgg
    11581 aggcgttcct tcatcatggc gattatcacg tcgtcgggga ccagcccgcc ggagtccatg
    11641 tactttttgg cggtctttcc gagcgcggtc ccgctcctga cgttctctct gaggatatcg
    11701 cccgtggata tgtgggcgag gcagtaacgg tccttgagaa ccgctgcctg tgtccctttg
    11761 ccggcgccgg gcgacccgag gaagatgact ctcatgacct cgccgttccc tctagaattt
    11821 gaggagcccg gcggtcttcc cctttgaccg cttcaggatg ccctcgtaat gcctcatcag
    11881 gagctgggcc tcgacctggt ggaccgtgtc gagggccacg cccaccacga tcagtacggc
    11941 ggtgccgccg aagtagaagg tggtgatccc catgaggcgg gtcatcaggg tgggaagcag
    12001 ggcgacgacg gcgaggaaga cggcgccgcc gagggtgatc ctcgacatca ccttctctat
    12061 gtactccgcc gtaggtttac ccggcctgat gcccaggacg aaccctccgt acttcttcat
    12121 gttctcggcc gtgtcgttgg ggtcgaagac gacggcggtg taaaagtagg agaagaagac
    12181 gatcagcacc acgtagagcg tcatgtagac aaggctgtcg ggcgacagga agcgctggat
    12241 caccgccgac acttcacctg aaaaaagtct ggctatggtg gaggggaaca gcaacaccga
    12301 cgacgcgaat attatgggga tgacgccggc ctggttgacc ttcaggggaa tgaaggtgct
    12361 ctgtccaccg tagaccttgt tcccgacgac ccttttcgcg tactggacgg gaagcctcct
    12421 ctgcccctcc tggaggagga cgcagcccgc gatgaccacg aggatcagga tgagagccag
    12481 gctgatgaca aggatgttca tctcacccat cctcaagagt gaccaggtcc tgatcatggc
    12541 ctcgggcagc ctggccacaa tcccggcgaa tatcaaaagc gagatgccgt tgcctatccc
    12601 gtggtcggag atctcctctc ccagccacat gaccaccagg gagcccgccg tgacggtgaa
    12661 gatcgccgcg acgaagccca cccatccccc cgagaatacg cccaggttcc tgagccagaa
    12721 ggtcatacct atggcctgca cggcggcgaa gaacaccgtg ccgagccggg tgtactggac
    12781 gatcttcttc ctgccctgct cgccctcctt ctggagcttt tccagggctg ggaacaccac
    12841 caccagcagc tgcatgacga tgctggcgtt gatgtagggg gcgacgccta gcgcgaatac
    12901 gctgaacctc ctgagggcgc ccccggcgaa gaggtccaga aacccgagaa ctccgccctg
    12961 gtcgaacagg ctggccatgg cgtcggggtc gatgcccggc gtggggatat gggcgccaag
    13021 acgaaagagg aagagcaccc ccatggtgaa gagtattttc ctcttgagat cgggcaacct
    13081 gaaagcatcc ctgaatgcgt cgagcaccta gatcacctcg gtcttcccgc ctgcagcctc
    13141 tatcttggcc accgcctggg cgctgaaagc gttggccttc accgtcagtg ccctggtaac
    13201 ctcgccgtca cccagtattt tcaccggcct tttcgcgtcc ccgatgagcc cggccatcag
    13261 catctcgggt atgcccactt cgccgctatc ctcgaaatgg tcacccagcc ttcccacgtt
    13321 caccagctgg aaggtttttt taaacctggc gttgctgaag cccctcttgg ggactctcct
    13381 cgtgaggggc atctggcccc cctcgaaacc ggggcgaacg cctccaccgc ttctggcttt
    13441 ctggcccttg gtgcccttac cggcggtcct gcccgtaccg gaccccgtgc cctgtcccat
    13501 cctttttggt tttctcctcg acccaggagc gggccggagc tcatgaattc ccacgagcct
    13561 ctccccccct attcctctac gatctcccag tccacaaggt gctccaccgc ggctatcatc
    13621 cctcggacct ggggagtgtc ttcatggatc acggtctgcc tgatattcct caggcccaat
    13681 gcccggaccg tctccccctg gcggggcggg cggcctatgg tgctctttct gagcgtgacc
    13741 ttcagcttgg ccatcgtcgt tctcgccccc tacgcttcct gggcggggcg ggcttccttg
    13801 ccgcggagcc tcatgacctc cgccggggag cggagcccct tgagtgccgt catggtggcg
    13861 taggccac
//
LOCUS       DAOSRG010000012        13813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4519_length_13813_cov_9.496075, whole genome shotgun sequence.
ACCESSION   DAOSRG010000012 DAOSRG010000000
VERSION     DAOSRG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_13813_cov_9.496075"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..768
                     /locus_tag="PLM30_01040"
     CDS             37..768
                     /locus_tag="PLM30_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53784.1"
                     /translation="MLYCTEERFQFFRLRSVSFDSLALVSDDQLWKRVEPKAHRFWPF
                     FSLKLGSIERSIMAEAPVDIDISRTGPGVFKVKAKPLEPWFMVFWAGGEWFLAREGRM
                     WSVHHSMNSIISQQSAREGAVLVWGRGLPDPVPSGIDIENSVKDSLIPCDEIESWKEA
                     LDNLGFYKKVSSITINRSEGRRTVEILERTSEGSVRILLDEFPAHWPPLLEAVNDILS
                     QTGLKDKNLLVDTTYSGKILVRVIS"
     gene            793..>1298
                     /locus_tag="PLM30_01045"
     CDS             793..>1298
                     /locus_tag="PLM30_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOR53785.1"
                     /translation="MRRGKVEPIIWSGLDIGTRKISLIVAETDPLSQDTQVIAVGSSR
                     TRGISKGEIRDADLVVESIRKAVDEAEKAFDLNVRDAFLSIGPTSVSSFILEHQCTLS
                     EEGITERPVTGKDMKDIIREAMKGAREITEDLLLHAIPLAYSVDAGPSVKDPRGALGS
                     ELATEVLF"
     assembly_gap    1299..1398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1399..2010
                     /locus_tag="PLM30_01050"
     CDS             <1399..2010
                     /locus_tag="PLM30_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53786.1"
                     /translation="GDLVTQDISEVLTIPASKAEYLKREVSLFESPEDLGDELEFEMG
                     GEPFVVTVQDVLGIVSPRIEEIFFRFIVPKIEEFEQEGTLSAIVFSGGVAGSKGFMDL
                     ASESFETPVRLGEPVKGTSLPPHARGCEFSSTVGIVNYLLERDNNPDNLLEPVFGALA
                     PEEAGMIKTPSPWKGKLVKKENEGKGPMARFFDALRNAFSELF"
     gene            2036..3187
                     /gene="ftsZ"
                     /locus_tag="PLM30_01055"
     CDS             2036..3187
                     /gene="ftsZ"
                     /locus_tag="PLM30_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOR53787.1"
                     /translation="MAELFEIEGSQSQLREVIKVIGVGGGGGNALNHIVRSGVTGVEF
                     IAANTDVAHLSLSEADVKIVLGRELTKGLGAGADPEIGMRSAEESIDEIREVLIGADM
                     IFLTAGMGGGTGTGASPVIAQVAKENGTLVVAVATKPFLFEGQRRITQALEGIENLRG
                     NVDALIIIPNDRLLEITQKETTLLDAFRLADDVLRQAVQGVTDLILRPGLVNVDFADV
                     RTIMKDAGTAIMGIGEAKGDDRASKAAHAAINSPLMDGPITGARGVLFNVTGGPNLGI
                     HEIQEAARVVTEAADPKATILWGHVLDPELDEKVQITVIATGFASGPGPRRALSIPRK
                     SGGPFIETEALARGDVSRQTAKDPVPPLENMEDLDTPPEIRRRKYGHKF"
     gene            3317..4738
                     /locus_tag="PLM30_01060"
     CDS             3317..4738
                     /locus_tag="PLM30_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR53788.1"
                     /translation="MLYPARYEVGMANLGYQTIISEMRTLGVGIERFFSGRLSSLSVE
                     AERSISDFPLISASIAYEPDILTLLSVFRGWGISPCPEERESQGQPVFGVGGALSYIN
                     PLVFSGVADYVVLGDGEKVVDHLVREARRYVRDGDRSKFRRALAEHPSIYVPPLHDRS
                     ILRGEKVHKHRNVIDNLDDARGKSLWVAPSSVFGKTVLVELQRGCRRNCSFCTIPSSF
                     GKPRTRSLARVLEDVSSVDSLDGVLVGLVSPETGDYPALEDLLSAVDSLGAAVSFASL
                     RVDNINEAMIKSLARSGRHTLTVAPEAGNERLRRACGKHFSDDLVIERLAMASHMGVR
                     KVKLYFMMGLPGETTSDMDDIARLCGRVRIESGLQVRASVGVFVPKPMTFWEKVEMIS
                     DDEAHSRKDRLSKLFRDGPGKGCRVNFQEPREAAVEFAIAWKGVSPANKFLSDPRGKA
                     SFPKPGPEQRDFLLEQLGLLGYH"
     gene            4779..5375
                     /locus_tag="PLM30_01065"
     CDS             4779..5375
                     /locus_tag="PLM30_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOR53789.1"
                     /translation="MSFRKNRKFKEKPAVFAFGHIMLPLVAILAIGLLILGVRLLFVS
                     PGGQVSSPDVIEYPEEESQAAQVITEPGDGPQVVAVPSTGGDEPTATPEVVPAAPAAE
                     ETPPTQPETETKAAPAQASRGSWVVQIGAFSLKESAETLAREVREKNFNAYVARAEVG
                     GKTYYRVRIPAGDQKAEADNLARDLASKGYPTLVTRQE"
     gene            5415..6653
                     /locus_tag="PLM30_01070"
     CDS             5415..6653
                     /locus_tag="PLM30_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOR53790.1"
                     /translation="MSGFVTNTLSREEALRALAQQMRFPWPVAVREGIPIEEALGLAL
                     GADVIAPEESPPFDRSLRDGYAVRSMDLIGTSDASPAFLALRGEVPMGTVPLMDVGSE
                     EAVLVHTGSAIPPGADSIVMLEDTSLAGNLLEVRKSVQKGENLLLKGEEYGRGQRLAR
                     KGEILDFRNIPALASCGQATTVVCDLGIAVISTGDEIVPADTNHIEPGMIRDANSFTI
                     MANLARAGFRSESLGIVPDDLENLKKAFNRALGEFDVVILSGGSSVSARDHSVLLLRE
                     LGGGKLIVRGLNISPGKPTIVSGDPARKKLGVCLPGHPHSCAVIASTFLVPLISSLIM
                     GEPQDLFRKILLPAGEDIIGRSGVEEFIPLKIGTEGEARPLWAKSGFLLGMGNSDGLI
                     RLPENMETLRKGAPVEVWLW"
     gene            6650..8557
                     /locus_tag="PLM30_01075"
     CDS             6650..8557
                     /locus_tag="PLM30_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR53791.1"
                     /translation="MTFKENLDLLTAWSLIETRVRPLDELEQIDLLSFEGYQPRILGV
                     DVLSRRNLPQYPASAVDGFALKASDTANASPRTPARVPEGSYGWVNTGGGVDRAFDSV
                     VMVEDTSMDGSTLLVAKMTTKGENVRPEGEDVTKGQVIARRGDPLDAFLIPLLIASGY
                     STASVPRLPRTIFIPTGDEIIPAAEWVRGDGGEWGRVPETNSYMLKKLFQDWGFPLEI
                     HGLVRDDEGLIREAALKSLEVYDLVIIGAGTAKGKRDHSAEVIEKLSDPLFRGIRMKP
                     GRPVIAGVRGNRPLVALPGFPMSALVTCWSVINPLLKRLYGETSAPAPEEAVGSTETL
                     NTKFLLHHSSQPGVLEWLRIKCGEIDGTIYSWPLPAGASSTFSIADADGFAILEPSTL
                     EVPKGSDIRILLRKKVDLTKRIVYQGSNDPAIERIVSYVRERGGDLAIRSVGSLGGLS
                     ALARGEGHIASCHLLDPEKGVYNDSYIERLDETRSWRRIKLFKRQQGFLVRKGNPAGI
                     HSVKDLAVTGAFFVNRQPGAGTRVLFDHLLKEAGIDPGSINGYDNIAITHYEAAARIP
                     GGSAVATLGVKAAAEAFGTDFIPVTEEDFELVIPERFVDHPGILILLEALSDERWRSE
                     VDSLGGYRWIS"
     gene            8545..9522
                     /gene="moaA"
                     /locus_tag="PLM30_01080"
     CDS             8545..9522
                     /gene="moaA"
                     /locus_tag="PLM30_01080"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOR53792.1"
                     /translation="MDFLKDRFERRLNYVRISITDRCNYRCVYCMPEEGLQWIPHERI
                     MTYEEIVFLAQALKGVGVEKIRFTGGEPLVRKGLFPFLETFSDSVPGVRTVLTTNGSL
                     IPQFIDEISRSGLSGVNVSLDTLDENLFRFITRGGRLQDVIEGVRLLSERTEIPIKIN
                     TVLMKGVNDGEVKALLDFARSHRAVLRLIEFMPLDDSVWNKDLFIPTSDIMQRLPDPD
                     RWEKLDNSSDHSAGPARYFRNSSTGQRLGLIEAVSHHFCDSCNRLRVSATGIMRPCLF
                     SEEGVFLRPALLSGDTAEVKALILRAVSCKPGSYKQARSGAMHMSRIGG"
     gene            9529..10014
                     /gene="moaC"
                     /locus_tag="PLM30_01085"
     CDS             9529..10014
                     /gene="moaC"
                     /locus_tag="PLM30_01085"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOR53793.1"
                     /translation="MDGFSHLDDSGRPVMVDVGGKDPTVREAMAEGIVIFPGKVFESL
                     VKGRNPKGDVLRVAEVAGIMGAKKTSALVPLCHPVRLDHVSVSCDLDPKGKMARITCT
                     ARARDVTGVEMEALTGVSVAALTLYDMCKGIDKGMVIEGIRLLSKSGGRSGEYRAEVG
                     P"
     assembly_gap    10160..10259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10260..10709
                     /locus_tag="PLM30_01090"
     CDS             <10260..10709
                     /locus_tag="PLM30_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOR53794.1"
                     /translation="ERVDTAGPALERSLPALGAVLKKTGMVPDETGKISDKIREWMTA
                     EVDIILVTGGTGISRRDVTPEAVKSLGCREIPGIGEYMRWKTSFENERSILSRSLAVA
                     CSGTIVITLPGSERGAVQCFSAVAPVLRHAVEVATGRSGDCGGSRRG"
     gene            10729..11613
                     /gene="rapZ"
                     /locus_tag="PLM30_01095"
     CDS             10729..11613
                     /gene="rapZ"
                     /locus_tag="PLM30_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOR53795.1"
                     /translation="MKESLPNRVSRCVIITGMSGSGKSTALRMFEDLGFYAIENIPPT
                     LLPQLLQVLGRHEEAVQNGVAAVVDIRGESLLDDLFAVIASLKDRGLNIRVIFLDASD
                     SCLVKRFEETRRRHPLSSEITILEAIGRERRELSQIHEIADVVVETSDLTLSQLRNEI
                     FAKMEITEGPLKVIFTSFGFKHGIPQDSDFVIDVRFLPNPFYESKLRPLSGRDREVQD
                     FILSHDGVSSFFSALSELMEQVTALYRNTGKSIVHIAVGCTGGRHRSVAVAEWLGSKM
                     PDNSSIRHRDIEKDTVVT"
     gene            11610..12749
                     /locus_tag="PLM30_01100"
     CDS             11610..12749
                     /locus_tag="PLM30_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOR53796.1"
                     /translation="MNWWSAFTIGFLTGGIIALLGARLFQEEVEEGAGESQDKKNGSV
                     MTKAFLHKVSQGPEIVAIGGGTGLSTLLSGLKYYTRNITAIVTVTDEGGSSGRLRGEW
                     GVLPPGDIRNCMLALAEDDSSLNRLLNYRFDRGELKGHSMGNLILLAATEMTGDFRKA
                     VEELNRLLAIRGKVIPVTNESVVLCAATADGDLLRGELDIASRGSRIESIWIEPADAR
                     AFPEALAAIENADAVVLGPGSLFTSIIPNLMFREVSEALKRTAAPKIYITNLMTQPGE
                     TDGMTLMDHIRWITRASGTIPDYILVNDEEVPSTLLETYRAAGASPLSLSEENERTLS
                     LMGSKLILSGFLDVAGGNVRHNSQKVSETLMRIAREERDSSRWKS"
     gene            12737..13642
                     /gene="whiA"
                     /locus_tag="PLM30_01105"
     CDS             12737..13642
                     /gene="whiA"
                     /locus_tag="PLM30_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOR53797.1"
                     /translation="MEKLREVIRDDWSAVLVDDSVKASVEISGILSGLVAGCDKEGMV
                     FSSRRFFLFRKLKKLWEISDWGRSGQTLNLIIPAGRKGRVSLRLDDETVLKGISYYGD
                     LHRVESLSWHWLRGLFGSCGNLSLPRTGYYMSFRVSESSQWDPIEKLEKVLSSKGISF
                     RKRQRRNFSEFVLRDQSSIVMMLSNMLMFKASLLLEEKALLRSIKERANKIVNCDASN
                     IRKSLESAQRQVELARMLLAKGDVLKLPDRLRELVYARLENPSATLNELGQALARPVS
                     KSTVKYRWGKIELLTRMSLKIEKGG"
     gene            13648..>13813
                     /locus_tag="PLM30_01110"
     CDS             13648..>13813
                     /locus_tag="PLM30_01110"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HOR53798.1"
                     /translation="MYLGKADVNTYEKGSSREFLTTNGWGGYGFSTVIGANTRREHGL
                     LVAKKSGEETF"
BASE COUNT         3084 a         3604 c         4093 g         2832 t
ORIGIN      
        1 attagggttg ccctgtttct ggttttcctt tcaggggtgc tatactgtac ggaagaacga
       61 ttccagttct tccgtctcag gtcggtctct ttcgattccc tcgccctggt ttcggacgat
      121 caactttgga aaagggttga accgaaggct catcgttttt ggcccttttt ttctcttaag
      181 ctgggatcca ttgaaagatc gatcatggcc gaggcaccgg tcgatataga tatttcacgg
      241 acaggtcccg gcgttttcaa agtgaaggca aagcctcttg aaccgtggtt catggttttc
      301 tgggccgggg gagagtggtt cctcgccagg gaggggagga tgtggtcagt ccaccactcc
      361 atgaacagca tcatttccca gcagtcggcg agggaggggg cagtccttgt ttggggtcgc
      421 ggcctgcctg accctgttcc ttccggtatc gacattgaga attccgttaa ggattcgctg
      481 ataccttgcg atgagatcga gtcttggaaa gaagctctgg ataaccttgg tttttacaaa
      541 aaggtctctt ccattaccat caacaggagc gaaggaagaa ggaccgtcga aatactcgaa
      601 aggaccagcg agggaagcgt aaggatcctc cttgacgaat tccccgccca ttggccgccc
      661 ctcctggaag cggtaaatga tatactttcg caaaccggct taaaggacaa gaacttgctt
      721 gtagatacga cctattccgg aaagattctg gtcagggtaa tatcctgatc tctacggtct
      781 ggaggttcgc cgatgaggag agggaaggta gagcccatca tatggtcggg tcttgatatc
      841 ggcaccagga agatctcgct gatcgtggcc gaaacggacc ccctctcaca ggatacccag
      901 gttatcgccg tcggatcctc tagaacgcgg gggatctcaa agggcgagat ccgggacgcc
      961 gatcttgtgg tcgagtccat caggaaagcc gttgatgagg ctgaaaaggc cttcgacctc
     1021 aacgtcagag atgccttcct ctcgatcggg cctacgtcgg tttcctcctt catcctggaa
     1081 catcagtgca ccttaagtga agagggtata acggaaaggc ccgtcacggg caaggatatg
     1141 aaagacatca tcagggaagc catgaagggc gcacgggaga tcaccgagga tctcctgctt
     1201 cacgcgatac ccctcgctta ttccgtagat gccggccctt ccgtgaaaga cccaagaggg
     1261 gccttgggat cggaattggc caccgaggtc cttttcatnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnngg agacctggtc acccaagata tttctgaagt gctcacaata
     1441 ccggcgtcaa aggccgaata cctgaagcgt gaggtatccc tttttgaaag cccggaagac
     1501 ctgggggatg aacttgaatt cgagatgggc ggggaacctt tcgttgtgac cgtccaggac
     1561 gttctcggga tcgtgtcgcc gaggatagaa gagatcttct tcaggttcat tgtccctaag
     1621 atagaagagt ttgaacagga agggaccctc agtgccatcg ttttttcggg cggcgttgcc
     1681 gggtcaaaag ggttcatgga cctcgcctca gaaagctttg aaacgccggt caggctcggt
     1741 gaacccgtaa aagggacgag cctcccacct cacgcaagag ggtgtgaatt ttcatccact
     1801 gtggggatcg tgaattatct acttgaaagg gacaacaacc ccgacaatct cctggaaccg
     1861 gtgttcggag ccttggcccc ggaagaggcg ggtatgatca aaactccgag cccctggaaa
     1921 ggaaaattgg tgaagaaaga aaacgagggc aagggcccga tggccaggtt cttcgacgcc
     1981 ttgaggaacg ccttcagtga gctgttctaa tccgtttatg gtggaggtta ctcgaatggc
     2041 tgaactcttt gagatcgaag gttcccagtc gcagttacgc gaagtgatca aggttatcgg
     2101 ggtgggcggg ggcggcggta acgccctcaa ccatattgtc aggagcggtg tgaccggcgt
     2161 ggagttcatc gccgccaaca ctgatgtcgc tcacttgagt ctctccgagg ccgacgtcaa
     2221 gatcgtgctg ggcagggaat tgaccaaagg cctcggcgcg ggcgcggacc cagagatcgg
     2281 gatgaggtcc gccgaagaat ccatcgatga gatcagggaa gtgctgatag gtgccgatat
     2341 gatctttctc acggcgggca tgggcggcgg gaccggcaca ggggcatcgc cggtcatcgc
     2401 gcaagtcgct aaggaaaacg ggacccttgt cgtggccgtg gcgaccaaac ctttcctttt
     2461 cgaggggcaa agaaggatca cccaggccct ggagggcatt gagaaccttc gtggcaacgt
     2521 ggatgccctc atcataatac cgaacgatcg cctcctggag atcacccaga aagaaacgac
     2581 cctcctggat gctttcaggc tcgccgacga cgtcctaagg caggctgtcc aaggtgtcac
     2641 agaccttatc ctccggccgg gccttgttaa cgttgatttc gcagatgtga ggacgatcat
     2701 gaaggatgcc ggaacggcca ttatgggcat cggcgaggcc aagggggacg acagggcttc
     2761 caaggcggcg catgccgcca tcaatagtcc gctgatggac ggccctatta ccggagctcg
     2821 gggggtgctc ttcaacgtca ccgggggacc gaatctgggt atccacgaga tccaggaggc
     2881 ggcccgagtc gtgacggaag cggcagaccc gaaagccacc atcctctggg gtcacgtact
     2941 tgaccccgag ttggatgaaa aagtgcagat caccgtgatc gcgacgggat ttgcctcggg
     3001 cccggggcca aggagagcgc tgtcgatccc gaggaaaagc gggggtccct ttatcgagac
     3061 tgaagccctt gcccgggggg atgtctccag gcaaaccgcc aaagacccgg tcccgcctct
     3121 tgagaatatg gaagacctcg atacccctcc agagatcaga aggaggaagt acggtcataa
     3181 gttttaaaac aacggatcgg gacaagcact gttgaagcgt gtttccggaa aaagccaaag
     3241 ggagacttgg aaatacctca gggagcagga agtagaactt gacgtccttc cccggggggg
     3301 ccatccgttg tgggccatgc tttaccccgc ccgctacgag gtggggatgg caaatctcgg
     3361 gtatcagacc ataatctcgg agatgcggac cctcggcgtc gggatcgaac gttttttttc
     3421 gggtcggctt tccagcctat cggtggaggc ggaaagatcg atctccgact ttcctcttat
     3481 ttccgcgtca atcgcctatg agcccgatat cctgaccctt ctttcggttt ttcgagggtg
     3541 ggggatatct ccctgtccgg aagaaaggga atcccagggc cagcccgtct tcggagtcgg
     3601 cggagctttg agttacatca atcccctggt tttttcagga gtggcggatt atgttgtttt
     3661 aggcgacggg gaaaaggtag tcgatcacct ggtgagggaa gccaggcgtt acgtacgcga
     3721 cggggaccgg tcgaagttca ggagggcact ggcggagcat ccatccattt acgtacctcc
     3781 tctgcatgac cggagcatcc tcaggggcga gaaggtccac aagcacagga acgttatcga
     3841 taaccttgac gacgcaagag gaaaaagcct ctgggttgcc ccctcctcgg tcttcgggaa
     3901 gactgtatta gtcgaattgc agaggggttg caggaggaat tgcagttttt gtaccatccc
     3961 ctcttccttc ggaaagccca ggacccgctc ccttgcgcgg gtcctggagg atgtcagttc
     4021 ggtggattcc cttgacgggg tactggttgg tttagtctcg cctgaaacag gcgactaccc
     4081 ggccttggag gacttgctat ccgccgtgga ttccctcggc gcggccgttt ccttcgcttc
     4141 cctcagggtt gacaatatca acgaagccat gatcaagtcc ctggcccgaa gcggaaggca
     4201 taccctcacc gtagccccgg aagccgggaa cgagcggttg agaagagcct gcggcaagca
     4261 tttttccgat gaccttgtta tagaacgact cgccatggcc tcccatatgg gcgtcagaaa
     4321 ggtaaagctt tatttcatga tgggactccc cggggagacc acttctgata tggacgacat
     4381 cgcccggttg tgcggccgtg tacgcatcga gagcggactc caggtcaggg cttcggtggg
     4441 cgtgtttgtt cctaaaccga tgaccttttg ggagaaggtt gagatgataa gcgacgatga
     4501 agcccattcc aggaaggata gattgagcaa attattcagg gatggtcccg ggaagggctg
     4561 ccgggtcaat ttccaggagc cgcgggaagc ggccgttgaa tttgccatcg catggaaagg
     4621 tgtatccccg gcaaacaagt tcctctccga tccgagagga aaagcaagct tccctaaacc
     4681 cggcccggaa cagagggatt tcctgctgga acaactgggc ttgcttggtt atcattaatt
     4741 atcctggtca gagaacacac tttgtgaggt gatcatcggt gagcttcaga aaaaacagga
     4801 agttcaaaga aaagccggct gtattcgctt tcggccatat tatgctgccc ctggtcgcca
     4861 tcctggccat aggacttttg atactcgggg taaggcttct tttcgtgtca cccggcggcc
     4921 aggtaagttc ccccgacgtg atcgaatacc ccgaggagga aagccaggcc gcgcaggtga
     4981 tcaccgaacc aggcgatgga ccccaggtcg tagctgtccc ctcaaccggt ggagatgaac
     5041 ccacggccac tcccgaagtc gtcccggccg cgcccgccgc cgaagagaca ccaccaaccc
     5101 aacctgaaac ggagaccaag gcggcgcctg cccaggcctc gaggggaagc tgggttgtcc
     5161 agatcggggc cttttccctt aaggaatcag ctgaaaccct cgcccgggaa gtgagagaaa
     5221 agaacttcaa tgcctacgtg gcccgcgcgg aagttggagg gaagacctac taccgggtga
     5281 gaatccccgc gggtgaccaa aaggccgagg ctgataacct tgccagggac ctcgcatcga
     5341 aggggtatcc tacgctggtc actcggcagg aataggagcc ggaagagatc gatgatcctt
     5401 ccgttcgaaa accaatgtca ggttttgtga ccaacactct ttccagggaa gaggccctgc
     5461 gtgcccttgc tcaacagatg aggtttcctt ggcctgtggc cgtgagagag ggtatcccta
     5521 tcgaggaagc cctgggcctc gctttgggag ccgatgtcat agcacccgaa gagagccctc
     5581 ccttcgacag gagccttcgg gacgggtatg ccgtgaggtc gatggacctg ataggtacaa
     5641 gcgacgcgtc acctgctttt ctggcactac gcggcgaggt gcccatgggt actgtcccac
     5701 tgatggatgt gggatctgaa gaggcggtcc tggtgcatac tgggagtgcc attccacccg
     5761 gggcggattc catcgtaatg ctcgaggaca cgtcccttgc cggaaacctg ctggaggttc
     5821 gaaaatccgt ccagaaggga gaaaatctcc tcctcaaggg tgaggagtac ggcaggggcc
     5881 aaaggttggc ccgcaaggga gagatcctcg attttcgcaa tatccccgcc ctggcatcct
     5941 gcggacaggc gaccacagtg gtatgcgacc tcggtatagc tgtcatcagc accggagacg
     6001 agatcgtacc ggctgacaca aatcatatcg agccagggat gatccgggac gccaactcct
     6061 tcaccatcat ggccaacctg gcaagagcgg gtttcaggag tgaatccttg ggaatcgtcc
     6121 ctgatgacct cgaaaatttg aaaaaagctt tcaacagggc cctgggtgaa ttcgacgtcg
     6181 tgatcctgag cgggggctcc tcggtcagcg ccagggacca ttccgtcctt cttcttcggg
     6241 aactgggagg gggaaaactg atcgtccgtg ggctgaatat ctcccctggc aaaccgacta
     6301 tcgtctcggg ggatcccgcc aggaaaaaac tcggtgtctg cctgccgggc catcctcact
     6361 cctgtgctgt gatcgcctcg accttcctgg tgccccttat ctcttcgctt ataatgggtg
     6421 agccccaaga tctttttcgg aaaatcctcc ttcccgctgg agaggacatt atcgggagga
     6481 gcggcgtgga ggaattcatc cccctgaaga tcggaaccga aggagaggcc agacccctgt
     6541 gggcgaaatc gggctttctc ctgggcatgg ggaactccga cggccttatc cggctgccag
     6601 agaatatgga gaccctgagg aaaggggccc ctgtagaggt gtggctttgg tgaccttcaa
     6661 ggagaacctg gatcttttga ccgcctggtc cctgatcgaa acgagagtgc ggcccctcga
     6721 cgagcttgag cagatcgacc tcctttcctt cgaagggtac caaccgagga tactgggggt
     6781 cgatgtcctt tcgaggagga atctccccca ataccctgcc tccgccgtcg acggtttcgc
     6841 cctgaaggct tcggacacgg ccaacgcctc cccgagaaca ccggccaggg tcccggaagg
     6901 cagttacggc tgggtgaaca ccggcggagg cgtggatcgg gccttcgatt cggttgtcat
     6961 ggtagaggac acttcgatgg atggaagcac cctgctcgtt gcgaaaatga cgaccaaggg
     7021 cgagaatgtc aggcccgagg gcgaggatgt gaccaagggt caagtcatag caagaagagg
     7081 ggacccgctt gacgccttcc tgatcccgct gctgatcgcg tcggggtatt ccacggccag
     7141 tgttccaagg ttaccgagga ccatcttcat acccaccggt gatgagatca tccccgccgc
     7201 cgaatgggtc aggggagatg gcggggagtg gggaagggta cctgagacca actcatacat
     7261 gctgaaaaag ctcttccaag actggggctt ccccctggag atccacggac tggtccgcga
     7321 cgatgaaggg ctgatcagag aagccgccct caagtccttg gaggtttacg acctggtcat
     7381 tatcggcgcc ggaacggcga aaggcaaaag ggaccacagc gcggaggtga tcgagaaact
     7441 ctcggaccct cttttccggg gcatcaggat gaaaccgggg aggcccgtca tagcgggggt
     7501 aagggggaac aggcctttgg tcgcgctacc agggttcccc atgtccgccc ttgtgacgtg
     7561 ctggagcgtc atcaacccgc ttcttaagag gctttacggc gagacctccg ctcccgcccc
     7621 tgaagaggcc gtcgggagta ccgagactct caatacaaag ttcctgctgc accattcctc
     7681 gcagcccggg gtcctggaat ggctcaggat caaatgcggg gagatcgacg gtacgatcta
     7741 ttcatggccc ctccccgccg gtgccagttc gaccttttcc attgccgacg ccgacgggtt
     7801 cgccatcctg gagccttcca cccttgaagt tcccaaaggg agcgatatac ggatcttgct
     7861 caggaaaaag gtggatctca cgaagaggat tgtctaccag ggctccaacg accctgccat
     7921 tgaaaggatc gtatcctatg tcagagaaag gggcggggac ctggccattc gatccgtcgg
     7981 tagcctcggc ggcctttccg ccctggccag gggagagggc catatagctt cctgccacct
     8041 cctggacccc gagaagggtg tatacaacga cagttacatt gaaagactgg acgaaactcg
     8101 gagttggcgg aggatcaagc ttttcaaaag acaacagggc ttcctggtca ggaaagggaa
     8161 ccctgcgggg atccattccg tcaaggacct ggccgttacc ggagcctttt tcgtaaacag
     8221 gcaacccggg gcaggcacca gagttctttt tgaccacctc ttgaaggaag ccgggatcga
     8281 ccccggttca atcaacgggt acgacaacat tgccatcacc cattacgagg cggccgccag
     8341 gataccgggc ggctcagccg tcgccaccct gggcgtcaag gcggcggcgg aggctttcgg
     8401 gacggatttc ataccggtga cggaggaaga tttcgaactg gtcatccctg agcgctttgt
     8461 cgatcacccg gggatcctga tcctattgga ggccctttcg gacgagaggt ggaggagtga
     8521 agtcgactcc ctggggggat accgatggat ttcttgaagg atcgctttga aagacggctt
     8581 aactacgtca ggatatcgat cacagaccgt tgcaattacc ggtgcgttta ctgtatgccc
     8641 gaagaaggcc tccaatggat cccccacgaa aggataatga cttacgagga gatcgttttt
     8701 ctggcccagg ccctgaaagg agtgggggtc gaaaagatcc gctttaccgg cggagaacca
     8761 ctggtgagaa agggtctttt cccctttctt gagaccttca gcgattccgt ccccggcgtt
     8821 cgcaccgtcc tgaccaccaa tggaagcctg atcccccagt tcatcgatga aataagccgg
     8881 tcgggccttt ccggcgtgaa cgtgagcctc gacacgctgg acgaaaacct tttccgcttc
     8941 atcacgaggg gcggcaggct gcaggacgtg atcgaaggcg tcagactcct gtcggagcgg
     9001 acggaaatac cgatcaagat aaacactgtc ctgatgaagg gggtaaacga cggcgaggtc
     9061 aaggccctgc tcgatttcgc taggtcccat cgggccgttc tccgccttat cgagttcatg
     9121 ccccttgatg attccgtctg gaataaagac ctgtttattc cgacttcgga tattatgcaa
     9181 aggctgcctg acccggaccg ttgggagaag ctggataatt cttccgacca cagcgcgggc
     9241 cccgcgcgtt atttcaggaa ctcgtccacc ggccagaggc tgggcctgat agaggcggtc
     9301 tcacatcatt tttgcgatag ttgcaacagg cttcgcgttt cagctacggg gatcatgaga
     9361 ccctgccttt tttccgaaga gggggttttc ctgaggcccg cgcttctttc cggtgacacc
     9421 gccgaggtaa aagccctgat cctccgtgcg gtgagctgca aacccggatc ctacaagcag
     9481 gcccgatccg gtgcgatgca catgtctcgg ataggggggt gagcaagcat ggatggtttt
     9541 tctcatcttg acgactccgg aaggcccgtc atggtcgacg tgggggggaa agatcccacg
     9601 gtgagggaag ccatggccga ggggatcgtg atattccccg gcaaggtctt cgaatccctg
     9661 gtgaaaggca ggaatcctaa gggggatgtc ttgagggtcg ccgaagttgc ggggataatg
     9721 ggggccaaaa agacatcagc cctcgtgccc ttgtgtcacc ctgtgaggct ggaccatgtt
     9781 tcggtctcct gtgacctgga cccgaaggga aagatggcga ggataacctg caccgccagg
     9841 gcaagggacg tgaccggggt cgagatggaa gcattgacgg gggtatcagt ggcggcgctg
     9901 accctttatg atatgtgcaa gggtattgac aaggggatgg tcatcgaggg gatccgcctg
     9961 ctaagcaaga gcggcggtag gtccggcgaa tacagggcgg aggtcgggcc atgaaaaaca
    10021 gggtcctggg tttttccggc cgatacagtg atcgtccgat ggtgtacctg catagcgaca
    10081 acgcggggaa cccgcttgcc gacggttcga ccgtggatct gatccttgag gaggccggga
    10141 aaggttcacc tgcggaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    10261 agagggttga tacagctgga cccgctctgg agaggtcctt gcccgccctg ggagccgtcc
    10321 tgaagaaaac ggggatggtc ccggacgaaa ccggcaagat cagcgacaag atccgggaat
    10381 ggatgacggc agaggttgac atcatactgg tcacgggggg aacgggcatt tccaggaggg
    10441 atgtaacccc tgaagcggtt aaatccctag gctgcagaga gatacccggc atcggagagt
    10501 acatgaggtg gaagacatcc tttgaaaacg agaggtccat cctttccagg tcccttgccg
    10561 tggcttgttc cggtacaatt gtgatcacgc tgccggggag cgagagggga gcggtgcaat
    10621 gcttcagtgc cgtcgccccg gttctccgcc acgctgtgga agtagcgacg gggaggagtg
    10681 gggattgcgg cggatctcgc cgaggataat tgaaagaagg gtcccgtttt gaaagaatcc
    10741 cttcctaaca gggtctcaag gtgtgtgatc atcacgggca tgtcaggaag cggtaaatcc
    10801 accgcactgc gcatgttcga agaccttgga ttttatgcca tcgagaacat ccctcccaca
    10861 ttgctacccc aactattgca ggtcctgggc cgacacgagg aagcggttca gaatggagtg
    10921 gcggctgtcg tagacatcag gggggaaagc ctccttgatg atcttttcgc cgtcatagcc
    10981 agcctcaagg atagaggact taatatcagg gtcatctttc tcgatgcctc cgactcatgc
    11041 ctcgtcaagc gttttgaaga gaccaggagg aggcaccccc tctcttcgga gataaccatc
    11101 ctcgaggcta ttggcaggga gaggagagag ctgtctcaga tccacgagat agccgacgtc
    11161 gtcgtcgaga catcggacct gaccctttct caactcagaa atgagatctt cgcgaagatg
    11221 gaaataaccg aaggccccct caaggtcatc tttacctcct tcggattcaa gcatggcatc
    11281 ccccaggata gcgattttgt catcgatgtc aggttcctgc caaacccatt ttacgaatct
    11341 aagctgagac ccttgagtgg aagggacagg gaagttcagg atttcatcct ctcccatgac
    11401 ggggtatcca gctttttcag cgccctttca gaactgatgg aacaagtcac ggccctttac
    11461 aggaacacgg gaaagagcat tgtccatatc gccgtcggct gtaccggagg caggcaccgt
    11521 tcagtggccg tcgccgaatg gctggggtca aagatgcctg acaattcgtc gatcagacat
    11581 agggatattg aaaaggatac ggtggtaacg tgaactggtg gtcggctttc accatcggtt
    11641 ttcttacggg ggggataatt gcacttcttg gtgcgagact cttccaggaa gaggtggaag
    11701 agggcgccgg ggaatcgcaa gacaaaaaga atggctccgt tatgacgaaa gccttcctgc
    11761 acaaggtctc acaaggtccc gaaattgtcg ctatcggtgg agggacgggc ctttcaactt
    11821 tgttatccgg gttgaagtat tacacccgca atataactgc tattgtgaca gttaccgatg
    11881 aggggggaag ttccggccgc ctgcgtggtg aatggggagt ccttcccccc ggggatatca
    11941 ggaactgcat gctggccctt gccgaggacg acagctctct caacaggctg ctcaactaca
    12001 ggttcgaccg cggggaactc aaggggcaca gcatgggcaa cctcattctt ctagcagcca
    12061 cggagatgac cggcgacttc cggaaggccg tggaagaact taacagactc ctggccatcc
    12121 gcggaaaggt gatacctgtc acgaacgagt cggtggtcct ttgcgccgcc acggctgatg
    12181 gcgacttact ccgaggtgag ctggacatcg ccagtagagg gagccggatc gaatcgatct
    12241 ggatcgaacc cgccgatgcc agggcttttc ctgaagccct cgccgccatc gagaacgctg
    12301 acgcggtcgt gcttgggccg gggagcctgt tcacaagcat catccccaac ctcatgttca
    12361 gggaggtttc cgaggcactg aaaaggaccg ccgcgccgaa aatatacatc accaacctca
    12421 tgacccaacc cggcgaaacg gacggaatga ccctaatgga tcacatccga tggataacca
    12481 gggcctcagg gacgatccct gattatatcc tcgtcaacga tgaagaagta ccttccacgt
    12541 tgctcgagac atatcgggcg gccggggcct cgcccctgtc actgagcgaa gagaacgaaa
    12601 ggaccctgtc cctcatgggg tcaaaactca tcctgtcggg tttcctcgat gtcgccggtg
    12661 ggaacgtcag gcacaacagc cagaaagtgt ccgagaccct gatgcggatc gccagggaag
    12721 agagggactc gtcccgatgg aaaagctgag ggaagtgatc cgtgacgact ggtctgctgt
    12781 cctagtggat gacagtgtca aggcctcggt tgagatcagc gggatactct ccggcctggt
    12841 ggccggctgc gataaggaag gaatggtctt ttccagcagg aggtttttcc tgttcaggaa
    12901 actgaaaaaa ctttgggaaa tttcggattg gggcaggtcc ggtcaaaccc tgaaccttat
    12961 catccctgcc ggcaggaagg gcagggtctc gttgagactt gacgatgaaa ccgtcctcaa
    13021 aggcatttcc tattatgggg acctccatag ggttgagtcc ctttcctggc actggcttag
    13081 aggcctcttc gggtcctgcg ggaacctgtc cttgccaagg acaggttatt atatgtcttt
    13141 cagggtgagc gagtcctccc aatgggaccc tatagaaaaa ctggaaaagg ttctttcctc
    13201 gaagggcatc tccttcagga aaaggcaaag gcgtaatttt tccgagttcg ttctgcgcga
    13261 ccagtcttcc atcgtgatga tgctttccaa catgttgatg ttcaaggctt cccttctact
    13321 tgaggagaag gcgttgctcc gatccatcaa ggaaagggcc aacaagatcg tgaattgcga
    13381 tgcatccaac atcaggaaat ccttggaatc cgcccagaga caggtcgagc tggcgagaat
    13441 gcttttggct aagggcgatg tcctaaaatt gccggacagg ttgcgggaac tcgtatatgc
    13501 caggttagaa aacccgtcgg ccacgctcaa cgagcttggt caagcccttg ccaggcccgt
    13561 tagcaaaagc acggtcaagt atcgatgggg gaagatcgaa ctactcacaa ggatgtcttt
    13621 aaaaattgaa aaggggggct gaagatcatg tacctgggaa aggccgatgt gaacacttat
    13681 gaaaaaggct ccagccggga attcctgact acgaacggct ggggcggtta cggcttttcg
    13741 accgtgatcg gtgcgaacac ccgccgcgaa cacggcctgc tggtggcaaa aaagagtgga
    13801 gaagagacgt tcc
//
LOCUS       DAOSRG010000013        13102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4788_length_13102_cov_9.602974, whole genome shotgun sequence.
ACCESSION   DAOSRG010000013 DAOSRG010000000
VERSION     DAOSRG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4788_length_13102_cov_9.602974"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1526)
                     /locus_tag="PLM30_01115"
     CDS             complement(159..1526)
                     /locus_tag="PLM30_01115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOR53799.1"
                     /translation="MTDVCDWLRERDLEGICGDILEQISMPLIAGSVVEDRLVVESAN
                     GFFGDLIGKACPELPEALDVLLSGLPGPKDLEPLLRAVTAKGWKTLCWPLVPGEMELD
                     LSCWSDGERFVAFFHDLSPHEFQRRLLGMGERLTQALGLFDAKGTIPQLLLELVEHVG
                     VDWAGIFVWDGVGQRWMLSLEEAHPGEYGLGEKQSALTFARKLSEAQGGPFGHQFGGL
                     AIQEGSKWWVPWACEGREWEPLMTEAGFGSLFCGVLLTGSAPAVLLCLARAGGVFAKI
                     NRGVLEALWPVFLSSAERNRAVEGITRLYPRDPVSGLFARDMTLGLMKVEMDRSRRYG
                     YPLTVMAVEVTNLDRVWATGGEEAVAETVQVLSREVLKNLRSVDIGGRVGDGRIVVLL
                     PHTPLEGAEVVSGRIRSRLGAISPSQSLSVETEVRIALRSDDCEEPEDLLARLGFMGD
                     GQGSS"
     gene            1583..2401
                     /locus_tag="PLM30_01120"
     CDS             1583..2401
                     /locus_tag="PLM30_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR53800.1"
                     /translation="MPKEPYQVKKGALDLETKRLDAPGTFGDGLVPVHIMEGPGTRAD
                     APLVVLLHGVHGCAGPAPGNKYGDLARMLLELGARCAIVETSRRRRDRETFCEDREAW
                     AHEAFRGKTFRQDHADAVAGIEAATAVAAPGKVWLFGFSLGGIHAVLAAGNGSLSFTP
                     AGIILAGSGMRVRPEAELALDLPILDTMPDGRLLAEAAGRMKAERLISFWGTLDSTFS
                     EEDCQALVEAAPLPPERKGFVVIEGADHAFRNYRGSPSLKPLELISSYLGRALF"
     gene            complement(2398..3180)
                     /locus_tag="PLM30_01125"
     CDS             complement(2398..3180)
                     /locus_tag="PLM30_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOR53801.1"
                     /translation="MAPVFSVIFVLFSALLGASMGSFLNVVAARTVEGRPWWGSERSR
                     CDICGKVLSAVELVPLLSWLGFSGRCRSCGAKIPARYLLVEVFGAAVGGLIAWKWGPS
                     ATAAFAAVVAAGLFLNALTDLYSGYVYDLLAWGLGLAGLALRVFGGWGALADGLLGAG
                     LGFAVIAVIIIVSRGGMGWGDASLAAGMGAALGWRLAAWGLYTGFMMGGLTALALLLS
                     RRVRRKDPIPLGPFLAAGGVLSMLTGSWFFSYLGMAPGWPWG"
     gene            complement(3264..3704)
                     /locus_tag="PLM30_01130"
     CDS             complement(3264..3704)
                     /locus_tag="PLM30_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53802.1"
                     /translation="MQMPMQISLDEVLSMLLARVDSLAFNDENLKTKFNILARAMYRK
                     GLISDEDIVDSIREEHRILADLGLIQEVPADDVIRTVAEGILQWVKGDVGAIKKAMED
                     YEKKLQELAKQQAEKPKIDVASPAVLQQLDMLSGKGKGKSKLIY"
     gene            3807..>4156
                     /locus_tag="PLM30_01135"
     CDS             3807..>4156
                     /locus_tag="PLM30_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR53803.1"
                     /translation="MRWDPVKYVNQAALTIRRELPTFALVSFGTFLVSFGIMALTLPY
                     RLPTSGVAGVAVLTNYAYGISPAWVVGVGNVILLAWGLKELSLRFVGWTVYAVALMTV
                     LLKVMEGMPHPQLN"
     assembly_gap    4157..4256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4257..4523
                     /locus_tag="PLM30_01140"
     CDS             <4257..4523
                     /locus_tag="PLM30_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR53804.1"
                     /translation="RRRQVIIISNHPGETADFITGELRRGVTILHGEGGFTHQERPAL
                     LCVLTPRQTVDLKRHLSVNDPKAFMIVSDASEVLGRGFKSWTSL"
     gene            complement(4548..5537)
                     /locus_tag="PLM30_01145"
     CDS             complement(4548..5537)
                     /locus_tag="PLM30_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53805.1"
                     /translation="MLSLVRGGTRGMFLRYRDESVLGALAGEFPLEEKRYKDALDHSD
                     EGQTLIFAFSRGGHLADSRVFLSDVGPTEILVFLLNGKYCDKVDEVRTLPRTILFRVD
                     GDFPEIIEQMRQDYDGKVGRIPFFLRWGDAGDWVAVMFTRNNLNRPIDMKDLFPEVLY
                     VKMPYEKLFRSLRSRAMSYFNEARGYADWKNLEIRVYDVWERYPLQAKRLRLVLDELE
                     IGLVLGEGRGKDYARILMQLIIYRFQLATFLEPEQVKGMLMGMEYGASGKRLVDLDLF
                     EGKKKYSWGARSEKGEDRAKAGARFRESLMEGLLPSTREELARIEKELEGLER"
     gene            complement(5556..6170)
                     /locus_tag="PLM30_01150"
     CDS             complement(5556..6170)
                     /locus_tag="PLM30_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR53806.1"
                     /translation="MGAVRDFLACFRMGLPLPVCLEGARGRCLIHVSDTPSAFYGDLD
                     KLLGYLEPLCLVHTGDLVDEVKLGLRPGDDALFRRKLKGLVKAMGKVPGERVTIVAGN
                     HDLAGEIHPAFPRAKVFERGVRLSLCGLDVALAHSREELPQPPAAFNLFGHNADVMPD
                     SEGVRYLNGVLGINVILVESGEVSVLPYPLYVDDARSCKRKRGL"
     gene            complement(6186..6659)
                     /locus_tag="PLM30_01155"
     CDS             complement(6186..6659)
                     /locus_tag="PLM30_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOR53807.1"
                     /translation="MADSGKSIEEEVLGVMERNLQSIWDGDVAAYRETTSEDCTFFEW
                     YISPQRIDGLDFHLRELRVHRLVVGGGPTRLGSEDQTLEHEVLNPHVQVYGPTAIVTF
                     TLMVRAASPGNVVHKSHNETRVFHDFGTAEKPEWKLVHCHKSPIATPDSLPVLRS"
     gene            complement(6701..7060)
                     /locus_tag="PLM30_01160"
     CDS             complement(6701..7060)
                     /locus_tag="PLM30_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOR53808.1"
                     /translation="MPTERTARWLRFAWVAGIVAFLVISIMPLGRSPLPEVAMVDKVA
                     HFGVFLVLALFPTAGGAVRTRTVVMVLVLLALASEGLQAMVPFRSYEAADVAADLLGM
                     LSGLALGAFLSSFRQRL"
     gene            complement(7148..7321)
                     /locus_tag="PLM30_01165"
     CDS             complement(7148..7321)
                     /locus_tag="PLM30_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR53809.1"
                     /translation="MAKAVVDRDLCIGCEACVGTCPVSAISMEEGKAQVDPDTCIECG
                     ACVAVCPVSAISQ"
     gene            complement(7463..8902)
                     /locus_tag="PLM30_01170"
     CDS             complement(7463..8902)
                     /locus_tag="PLM30_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOR53810.1"
                     /translation="MTRCVCIHGHFYQPPREDPWTGRVGRQVSALPYHDWNERVTIQC
                     YGPNAAAWLLDGSGKVRRRLNNYSRMSFNFGPTLLSWMEEASPETYSAILSADGESAG
                     RFSGHGGAMAQAYNHMIMPLASARDKRTQVAWGKRDFRHRFGRDPEGMWLPEVAVDLE
                     TLEVMAEAGILFTILAPHQASRVREGGSWRDVTGGGFDTTVPYRCELSSGRSIALFFY
                     NGALANDIAFGNLLENGGTFAGRMIDGARGEGARLSHAAVDGETFGHHRRFGEMALAY
                     CLETVEEGGGAELTVYGEFLEKHPPTAVVEIIGNTSWSCTHGVERWRSDCGCRAGDHS
                     EWHQRWRKPLRESLDRLREEVDRLFEEAGGECFDDPWEARDGYIDVLLGGGRPGGRER
                     DLLEMERFAMLMYTSCGWFFDDISRIETVQVLGYAARAAQLAGEVSGKDVLGPFLKSL
                     EEVPGNTEEVPDARRALEAAFGERLGIAG"
     gene            complement(9056..>9896)
                     /locus_tag="PLM30_01175"
     CDS             complement(9056..>9896)
                     /locus_tag="PLM30_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOR53811.1"
                     /translation="FPGIETFAEESTAWPMVTRPVHTGGLGFGLKWNMGWMHDTLSYL
                     SKDPVFRKHEHDRLTFSAWYAFSENFVLPLSHDEVVHGKGSLFGKMPGDEWQKFAHLR
                     LLFGYMFAHPGKKLLFMGGEFAQVGEWDHEGQLEWELLEREPNGGLRRWVGDLNRVYK
                     AKKALHEQDCSPEGFRWVDARDRDNSVISFLRFSKGGEAVLAVCNFTPVPRHGYVLGV
                     PRGVTWREVLNSDAAVYGGSGLGNLGGVEAVEESSHGFPWRVSLTLPPLGMLLLEAEG
                     QRP"
     assembly_gap    9897..9996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9997..10823)
                     /locus_tag="PLM30_01180"
     CDS             complement(<9997..10823)
                     /locus_tag="PLM30_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR53812.1"
                     /translation="MRYDTSLLTEQDIYLFREGRHCLLYERLGAHFLDPGGEAGTHFA
                     LWAPSAKGVSVVGDFNGWDEKVCTLKPRWDSSGIWEGFVPGVRPGALYKYFIRSPGGD
                     ALKGDPFARRWETPPGTASVVWDGSHAWGDGRWMAERAGRKALESPLSIYEVHPGSWR
                     RDAGGGFLSWREMAPLLAGYAVEMGFTHVELMPVMEHPFYGSWGYQVTGYFAPTSRFG
                     SPEDLMCLVDTLHRAGIGVILDWVPSHFPDDPHGLARFDGTHLFEHADPRKGFHPDW"
     gene            complement(10850..11233)
                     /locus_tag="PLM30_01185"
     CDS             complement(10850..11233)
                     /locus_tag="PLM30_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53813.1"
                     /translation="MRSKRVKWAAVLAAAMLLGVASAAMAQSSYTGTYRGTFIGDDYG
                     WFVVEVEPGGTISGTVHSNVALMDLEVQGRWELGGDCEFMTVGGEGASIYFLGRIDFM
                     NRFLGKWSFDDHSGRGSFYGILQKD"
     gene            complement(11297..11827)
                     /locus_tag="PLM30_01190"
     CDS             complement(11297..11827)
                     /locus_tag="PLM30_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOR53814.1"
                     /translation="MREEELTESIEMYMGRLKSLGFRLTNQRRIIVETLLRHLGTHLN
                     ARELLELAQREDPSIGIATIYRTIELLNSLGMLNMVNLEEGFLRFEVPDEQMHFHVFC
                     RSCGKLVHLDDENRKMAVVKEWAEREGFELLPQTFEMAVICAECREKGVLDEPFEVFG
                     MGGRRRCRRHHGGRMR"
     gene            complement(11861..>13102)
                     /locus_tag="PLM30_01195"
     CDS             complement(11861..>13102)
                     /locus_tag="PLM30_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOR53815.1"
                     /translation="WVPPSLATLSEEMEKAHAQVEVLSSRAASIAQGVKHLEAAVPEG
                     LKEAELTAYIDTALKKREALELKASETKGLLEKAQKEHEALKAEYEGRFPGRPDRIVF
                     VTFSTEGKGQVLLTARTDSARWRPLYRLELDSSTGEIRGVYGVEVNQKSGIDWDGEVV
                     FHTVRPRGGVSIPDMPPLIADIHDPTKNAKGFALAMRAAAPAQDVAAGEYLEEGLTDV
                     AIRTSAAVNGSGEDVTIDAGTFTEKGEVSLVCIPEYSPEAWTVATVKSLGRALLPGEA
                     RLSVDGKDTGRTRLEALSMGQEAVLPFGTTPLVTAKREEMIPTTGTSWIVKGKHQRGY
                     IITVSNGLDKAVKVRVKDRVPVPANEAIKVQDVILSPAPAEREEKGFLTWEIALDKGK
                     SSQVSVKYTVTYPSDKELIFR"
BASE COUNT         2206 a         4646 c         3794 g         2256 t
ORIGIN      
        1 ggaggggaaa ccttttcgag atcctggccc gcctggggat cgacagcgaa cccgggtccg
       61 cctacctgga cctcgacgat tcggccgtca ggctcagggt gcctcccccg gaagacctgg
      121 aaagggccgt ggagttcctg tcggggcttt ccgacctgtc atgaggaccc ctggccgtcg
      181 cccatgaagc cgagccgggc gagaaggtcc tcgggctcct cgcaatcgtc gctcctgagg
      241 gcgatcctca cttccgtctc caccgacagg ctctgcgacg gcgatatcgc ccccagccgg
      301 gaccttatcc tcccggagac cacctcggca ccctcgagag gtgtatgggg cagcaggacg
      361 acgatccggc cgtcgcccac cctcccgccg atgtccacgc tcctgaggtt cttcagcacc
      421 tcccgggaga gcacctggac cgtctcggcg acggcctcct caccgccggt ggcccagacc
      481 ctgtcgaggt tggtgacctc caccgccatg accgtaaggg ggtacccgta gcgcctggag
      541 cggtccatct ccaccttcat gagccccagg gtcatgtccc gggcgaaaag acccgatacg
      601 gggtcgcggg ggtaaagcct ggtgatcccc tccacggcgc ggttcctctc ggccgaggag
      661 aggaacacgg gccaaagggc ctccagcacg cccctgttta tcttcgcgaa gaccccaccg
      721 gccctggcga ggcagaggag gaccgccggc gccgagccgg tcagcagcac cccgcagaag
      781 agtgacccga agccagcctc cgtcatcaag ggttcccact cgcgcccctc gcaggcccag
      841 gggacccacc acttcgaccc ctcctggatc gccaggccgc cgaactggtg gccgaagggc
      901 cctccctgag cctcggacag tttcctggcg aaggtgaggg cgctctgctt ctcgccgagc
      961 ccgtactccc cggggtgggc ctcttccagg gagagcatcc acctctgccc aaccccgtcc
     1021 cagacgaaga tgccggccca gtccactccc acgtgctcca ccagttccag gagcaattga
     1081 ggtatggtgc ccttcgcgtc gaagagcccg agggcctggg tgaggcgctc ccccataccc
     1141 aggagcctgc gttggaactc gtggggggac aggtcgtgga agaaggccac gaaacgctcg
     1201 ccgtcgctcc agcagctgag gtcgagctcc atctccccgg ggacgagggg ccaacacaag
     1261 gtcttccatc ccttcgccgt gaccgcccgc aggaggggtt ccaggtcctt gggccccggc
     1321 agcccggaaa gcaggacgtc cagggcttcc ggcagttccg ggcaggcctt gccgatgagg
     1381 tcgccgaaaa aaccgttggc cgactccacc accagtcgat cctcgacgac gctcccggcg
     1441 atcagcggca tggatatctg ttccaggata tccccgcaga tcccttccag gtccctctcc
     1501 ctgagccagt cgcagacgtc ggtcacgatc ggtgagcctc cccatggtaa gtgcttcgcc
     1561 tcgtccctat gataaactct agatgcccaa agagccatac caagtgaaga aaggggccct
     1621 cgacctggag acaaaaaggc tggacgcgcc cgggactttc ggggacggcc tcgtccccgt
     1681 ccatatcatg gaagggccag ggacccgcgc cgacgccccc ctggtggtgc tcctccacgg
     1741 cgtccatggg tgcgccggtc cggccccggg gaacaagtac ggcgacctcg cccggatgct
     1801 cctcgagctc ggtgccaggt gcgccatcgt ggagacgagt cgccggaggc gggaccggga
     1861 gaccttctgc gaggacaggg aggcctgggc ccacgaggcc ttccggggca agaccttccg
     1921 tcaggaccac gccgacgccg tggcgggcat cgaggcggcc accgctgtag ccgcccccgg
     1981 caaggtctgg ctcttcgggt tctccctcgg ggggatccat gccgtcctgg ccgccggcaa
     2041 cggctccctt tccttcaccc ccgccggcat catcctggcc ggttcgggga tgagggtccg
     2101 ccccgaggcg gaactcgccc tggacctgcc gatcctcgac accatgcccg acggtcggct
     2161 cctggccgag gccgcggggc ggatgaaagc ggaaaggctc atctccttct ggggcacgct
     2221 ggactccacc ttcagcgagg aggactgcca ggccctggtg gaggcggccc cattgccgcc
     2281 ggaaaggaag ggcttcgtcg tcatcgaggg ggcggaccac gcctttcgca actacagggg
     2341 gtccccctcc ctgaaacccc tggagctgat ctcctcctac ctgggccggg cgcttttcta
     2401 accccagggc cagcccggcg ccatgccgag gtacgagaaa aaccatgacc ccgtcagcat
     2461 cgacaggacc cctcccgccg ccaggaacgg ccccaggggg atggggtcct ttcgcctgac
     2521 cctccgcgag aggagcaggg caagggccgt caggcctccc atcatgaaac cagtgtagag
     2581 gccccaggcc gccaggcgcc accccagggc ggcccccatc ccggccgcca ggctggcgtc
     2641 gccccaaccc atgcctcccc ggctgacgat gatgatcacc gctatgacgg cgaaccccag
     2701 ccccgcgccc agaaggccgt ccgccagggc gccccaccct ccgaagaccc gcaaagccag
     2761 ccccgctagg ccgagccccc aggcgaggag gtcgtagaca tagccgctgt agaggtccgt
     2821 cagggcgttc aggaagagcc ccgcggccac cacggcggcg aaggccgccg tcgccgaggg
     2881 tccccacttc caggcaatga ggccgcccac cgcggccccg aagacttcca ccagcaggta
     2941 cctcgcgggg atcttcgccc cgcaggagcg gcaccgccct gagaagccca accaggacaa
     3001 aagaggtacc agctccacgg cggacagcac cttgccgcag atgtcgcacc gggaacgctc
     3061 gctcccccac caggggcggc cctccacggt cctcgcggcc accacgttga ggaaggaacc
     3121 catggaagcc cccaggaggg ccgagaaaag gacgaagatg accgaaaaga ccggagccaa
     3181 ccgcaccacc ccccgccgcg ccggcggccc ttatgaaaaa gggtcccggc gaacgccggg
     3241 acccttccga agcgaggcca agatcagtag atgagcttgc tcttcccctt gcctttaccg
     3301 ctgagcatgt ccagttgctg gaggaccgcc ggcgaggcca cgtcgatctt cggcttctcc
     3361 gcctgctgct tcgccagttc ctgcagcttc ttctcgtaat cctccatggc tttcttgatg
     3421 gcccctacgt cgcccttgac ccactggagg atcccctcgg cgacggtacg gatcacgtcg
     3481 tcggcgggga cctcctggat gaggcccagg tcggcaagga tcctgtgctc ctcgcggatg
     3541 gaatcgacga tgtcctcatc ggagatcagg cccttgcggt acatggccct ggccaggatg
     3601 ttgaacttcg tcttcaggtt ctcatcgttg aaggccaggc tgtccaccct ggccaggagc
     3661 atggacagca cctcgtcaag acttatctgc atcggcatct gcattttttt ccccaaatcc
     3721 ccctttgcta cggaatcggc cccattgtaa cacgtgggca acccacgggc tttgaagcct
     3781 cgcacggagg gattatactc tatcccatga gatgggaccc cgttaaatac gtgaaccagg
     3841 ccgcgttgac gatccgaagg gaactgccga cgtttgccct cgtgtccttc ggcaccttcc
     3901 tggtctcctt cggcatcatg gccctgacgc tcccctaccg cctccccacc tcgggcgtgg
     3961 cgggggtcgc cgtcctcacc aactacgcct acggcatctc cccggcgtgg gtggtggggg
     4021 tgggcaacgt catcctcctc gcctgggggc tcaaggagct ctccctccgc ttcgtggggt
     4081 ggaccgtcta cgccgtggcc ctcatgacgg tgctcctcaa ggtcatggag ggcatgcccc
     4141 acccccagct caacgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga
     4261 ggaggcaggt tatcatcatc agcaaccacc ccggtgagac cgctgacttc atcaccggcg
     4321 aactgcgccg gggcgtcacg atcctccacg gcgagggcgg tttcacccac caggagcgac
     4381 cggccctgct ctgcgtcctg accccgcggc agacggtcga cctgaaacgc cacctctccg
     4441 tcaacgatcc caaggccttc atgatcgtct ccgacgcctc ggaggtcctc ggccgcggct
     4501 tcaagagctg gacctccctc tagaccccgc ggcggggcaa aagagggcta ccgctccagc
     4561 ccttccaatt ctttctctat cctggccagt tcctccctcg tcgagggcag cagcccctcc
     4621 atcaggctct ccctgaacct ggcgccggcc ttcgcccggt cctcgccttt ttcggacctg
     4681 gcgccccagc tgtacttctt cttcccctcg aaaaggtcca ggtccaccag gcgcttcccg
     4741 gaggcgccgt actccatccc catgagcatg cccttcacct gctcgggctc caggaaggtg
     4801 gccagctgga accggtagat gatcagctgc atcaggatgc gggcgtaatc cttcccccgg
     4861 ccctcgccca gcacgaggcc tatctccagt tcgtccagca cgaggcggag cctcttcgcc
     4921 tggagggggt acctctccca cacgtcgtaa acccggatct ccaggttctt ccagtcggcg
     4981 tacccccggg cctcgttgaa gtaggacatg gccctgctcc ggaggctcct gaagagtttc
     5041 tcgtagggca tcttcacgta gagcacctcg gggaagaggt ccttcatgtc gatcggtcgg
     5101 ttcaggttgt tccgggtgaa catgacggcc acccagtcgc cggcgtcgcc ccagcgcagg
     5161 aagaagggga tcctcccgac cttcccgtcg taatcttgcc tcatctgctc gattatctcc
     5221 gggaagtccc cgtccaccct gaacagtatg gtcctgggaa gggtcctcac ctcgtccacc
     5281 ttgtcgcaat acttcccgtt caggaggaag accaggatct ccgtgggccc cacgtcggag
     5341 aggaagaccc tcgagtccgc caggtggccg ccccggctga aggcgaatat gagcgtctgt
     5401 ccctcgtcgg agtggtccag cgcgtccttg tagcgcttct cctccagggg gaactccccg
     5461 gccagcgccc ccaggaccga ctcgtccctg tacctgagga acatccccct cgtgccgccc
     5521 ctcacaagcg aaagcatccc ctcacccctc cctggtcaca ggcccctttt ccgcttgcag
     5581 gacctggcgt cgtccacgta gagggggtag ggcagcaccg acacctcacc gctttcgacg
     5641 aggatcacgt ttatcccgag gacgccgttg aggtaacgga cgccctcgct gtcgggcatc
     5701 acgtcggcgt tgtgcccgaa gaggttgaag gccgccggcg gctggggcag ttcctcccgg
     5761 gagtgggcca gggccacatc cagtccgcag agggacagcc tcaccccccg ctcgaagacc
     5821 ttcgcccggg ggaaggccgg gtggatctcc ccggcgaggt catggttccc ggccactatg
     5881 gtgaccctct cgcccgggac cttgcccatg gccttcacca ggcccttcag cttgcgccgg
     5941 aaaagggcgt cgtcgcccgg cctcaggccc agtttgacct cgtccaccag gtcacccgtg
     6001 tggacgaggc aaaggggttc gaggtaccct aggagtttat cgaggtcgcc gtagaacgcc
     6061 gacggcgtgt cggagacatg gatgaggcac ctcccccggg ccccctccag gcagaccggc
     6121 aggggcagtc ccatcctgaa acaggccagg aagtccctta cggcccccat gccgctgtca
     6181 tccccctagg acctcaggac gggcaggctg tccggggtgg cgatgggcga cttgtggcag
     6241 tggaccagct tccactcggg cttttccgcg gtgccgaaat catggaagac cctggtctcg
     6301 ttatggctct tgtgcaccac gttccccggc gatgcggccc tcaccatgag ggtgaaggtc
     6361 acgatcgccg tcggcccata gacctggacg tggggattca ggacctcgtg ctccagggtc
     6421 tggtcctccg accccagccg ggtggggccg ccgccgacca ccagccggtg cacccgtagt
     6481 tccctcaggt ggaaatccag gccgtctatc ctctgggggg aaatgtacca ctcgaagaaa
     6541 gtgcagtcct cggaagtggt ctcccggtag gctgccacgt ccccgtccca tatgctctgc
     6601 aggttcctct ccattacgcc gaggacttcc tcctcgatcg acttgcctga gtctgccatg
     6661 gcacttcacc agccttcatc atcgatattg tcgtcgaaca ttataaccgc tgccggaaac
     6721 tggaaagaaa agcacccagg gcgagcccgg aaagcatgcc cagcagatcg gccgccacat
     6781 cggcggcctc gtagctcctg aagggaacca tcgcctggag tccctccgag gccagggcca
     6841 ggaggacgag caccatcact accgtccttg tcctgacggc accgccggcc gtcggaaaga
     6901 gcgccaggac gagaaagacc ccgaagtgcg ccaccttgtc gaccatcgcc acctcgggga
     6961 ggggcgaccg gcccaagggc atgatcgaga taaccaaaaa ggccacgatg cccgccaccc
     7021 aggcgaacct cagccatcgg gcggtccttt cagtcggcat gggcagtgtc cctcgaaaga
     7081 taaaagataa aaaaagaggg gccccttccc tggggaagcg cccctcgaaa gaacgttacc
     7141 ccgtcaacta ctgggaaata gcactcacgg ggcagaccgc cacgcaggca ccgcactcga
     7201 tgcaggtatc gggatcgacc tgggccttcc cctcctccat gctgatggcc gacacggggc
     7261 aggtccccac gcaagcttcg caaccgatac aaagatccct gtcaactact gcctttgcca
     7321 taaagcttcc ctccttgtat ggggtgatcc gattccaacg ttcgcagtat cgtttatttt
     7381 agcgtaaatt tcaagggcgc caaaccctcg cccctgacat cgcgtcgaac catgggtgaa
     7441 gcgatgggcc ttcgaagggg cctcaccccg cgatgccaag cctctccccg aaggcggcct
     7501 ccagggctct cctggcgtcg gggacctctt ccgtgttgcc cggcacctcc tccagggact
     7561 tcaaaaaggg ccccagcaca tccttcccgg agacctcccc cgccagctgc gccgccctgg
     7621 cggcgtaacc cagcacctgg accgtctcga tcctggaaat gtcgtcgaag aaccacccgc
     7681 agctggtgta catgagcatg gcgaatcgct ccatctccag caggtcccgc tcccgccccc
     7741 ccggccttcc gccgcccagg aggacgtcga tgtacccgtc ccgggcctcc caggggtcat
     7801 cgaagcattc gcccccggct tcctcgaaga ggcggtccac ttcttcccgg aggcggtcca
     7861 gggactccct caggggcttc ctccaccgct ggtgccactc ggagtggtcc ccggccctgc
     7921 acccgcagtc ggacctccaa cgctccacgc cgtgggtgca gctccaggac gtgttcccga
     7981 tgatctcgac caccgccgtc ggggggtgct tctccaggaa ctcgccatag acggtcaact
     8041 ctgctccgcc gccctcctcc accgtctcca ggcagtaggc cagggccatc tcgccgaagc
     8101 gtcggtggtg gccgaaggtc tcgccgtcga cggccgcgtg ggagagcctc gccccctccc
     8161 cccgggcccc gtcgatcatc cggccggcga aggtaccccc gttctccagg aggttgccga
     8221 aggcgatatc gttggccagc gcgccgttgt agaagaagag ggctatgctc ctccccgagg
     8281 acagttcgca ccggtagggc accgtcgtgt caaacccccc gccggtgaca tcgcgccagg
     8341 accccccctc ccgaaccctc gatgcctggt ggggcgccag gatcgtgaaa aggatccccg
     8401 cttccgccat gacctccagc gtctccaggt ccacggccac ctcgggcagc cacataccct
     8461 ccgggtcccg cccgaagcgg tgccggaaat ccctcttccc ccaggcgacc tgggtcctct
     8521 tgtccctggc cgacgccagc ggcattatca tgtggttgta ggcctgggcc atggccccgc
     8581 cgtggcccga aaagcgcccc gcactctcac cgtcggcgga gaggatggcc gaataggtct
     8641 ccgggctggc ctcctccatc cacgaaagca acgtggggcc gaagttgaaa ctcatcctcg
     8701 aatagttgtt cagcctccgg cgcaccttcc ccgaaccgtc aaggagccag gccgccgcgt
     8761 tgggtccgta gcactggatg gtcacccgct cgttccagtc gtggtagggc agcgcgctca
     8821 cctggcgccc caccctcccc gtccaggggt cctccctggg tggttgataa aaatgcccgt
     8881 ggatacaaac gcacctggtc ataaaactgt ctcacgcccc taggttttcc ctgttgcctg
     8941 acgcctctcc tgtcgccttg cccttcggtc taccgctctt aaggttttgt ccttccgatt
     9001 aacgatttac gattcacggt ttttggtttc cgattcacga ttcacgattc acgatttacg
     9061 gcctttgccc ttctgcctcc aataaaagca ttcccagcgg cggcaacgtc agcgagaccc
     9121 tccagggaaa accgtgggag gactcctcca cggcctcgac cccgcccagg ttgcccaacc
     9181 cgctcccgcc gtagaccgcg gcgtcgctgt tcagcacttc cctccaggtc acgcccctgg
     9241 gcacccccag gacgtagccg tgcctgggca cgggcgtgaa gttgcagacc gccagcacgg
     9301 cctcccctcc cttggagaag cgcaggaagg agatgacgct gttgtcgcgg tccctggcgt
     9361 cgacccaccg gaaaccttcc ggggagcagt cctgttcgtg aagggccttc ttcgccttgt
     9421 agaccctgtt caggtccccc acccagcgcc ggaggccgcc gttgggctcc cgctccagca
     9481 gctcccactc cagttgcccc tcgtggtccc actcccccac ctgggcgaac tcaccgccca
     9541 tgaagagcag cttcttgccc gggtgggcga acatgtaccc gaacagtagc cgcaggtggg
     9601 cgaatttttg ccactcgtcg ccgggcatct tcccgaagag cgagcccttc ccgtggacca
     9661 cctcgtcgtg ggagaggggc agcacgaagt tctccgaaaa ggcgtaccag gccgaaaagg
     9721 tcagccggtc gtgctcgtgc ttccggaaga cggggtcctt agacaggtag gagagggtgt
     9781 cgtgcatcca ccccatgttc cacttcagcc cgaagcccag ccccccggtg tggacgggcc
     9841 gggtcaccat gggccacgcc gtcgactcct cggcgaaggt ctcgatgccc gggaagnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcc agtcggggtg gaagcccttc
    10021 ctcgggtcgg cgtgctcgaa gaggtgggtc ccgtcgaacc tcgccagccc gtgggggtcg
    10081 tcggggaagt gggagggcac ccagtccagg atcaccccta tccccgccct gtggagggtg
    10141 tcgacgagac acatcaggtc ctcggggctc ccgaagcggc tcgtcggggc gaagtaccct
    10201 gtcacctggt atccccagga gccgtagaag ggatgctcca tcacgggcat cagttccacg
    10261 tgggtgaagc ccatctccac ggcgtacccg gccagcaggg gggccatctc gcgccaggac
    10321 aggaatcccc cgccggcgtc gcgcctccag gaccccgggt gcacttcgta gatcgaaagg
    10381 gggctctcca gggccttccg cccggcccgc tcggccatcc accggccgtc cccccaggcg
    10441 tgggagccgt cccagaccac cgaggccgtc ccgggcggcg tctcccagcg gcgggcgaag
    10501 gggtcgccct tcagggcgtc gccgccgggc gaacggataa agtacttata gagggctccc
    10561 ggccgaaccc ccggcacgaa gccctcccag atacccgagg agtcccaccg gggcttcagg
    10621 gtacagacct tttcgtccca gccgttgaag tcgcccacca cggagacgcc cttcgccgac
    10681 ggcgcccata aagcaaagtg ggtccccgct tcaccccccg ggtcaagaaa atgggcgccg
    10741 agcctctcgt aaagcaggca atggcgccct tccctgaaaa ggtatatatc ctgctccgtc
    10801 aggagcgagg tgtcatagcg catggggccg tcgctgaccg cccggggcct cagtccttct
    10861 gcaggatccc gtaaaaggaa cccctcccgg agtggtcgtc aaaggaccac ttgccgagga
    10921 agcggttcat gaaatcgatc cgccccagga agtagatgga ggcgccctcc ccgccgacgg
    10981 tcatgaactc gcagtccccg cccagttccc accttccctg tacctccagg tccatgaggg
    11041 ccacgttgga atggacggtc ccgctgatgg tcccgccggg ctctacctct acgacgaacc
    11101 agccgtagtc gtcgccgatg aaggtccccc tgtaggtccc cgtgtaggag gactgggcca
    11161 tcgccgctga tgcaaccccc aggagcattg cggccgccag aaccgccgcc cacttcaccc
    11221 ttttcgacct catactccat cactcccttt caaggatgtt tccggtcccg agataatacc
    11281 acatcgccct gatatttcag cgcatcctgc ccccgtggtg cctcctgcac cggcgcctgc
    11341 cgcccatgcc gaagacctcg aagggctcgt cgaggacgcc cttctcccgg cactcggcgc
    11401 agatcaccgc catctcgaag gtctggggca gcagttcaaa accctcccgc tcggcccact
    11461 ccttcacgac ggccatcttt ctgttctcat cgtcgaggtg caccagcttg ccgcaggacc
    11521 ggcagaagac gtggaaatgc atctgctcgt cgggcacctc gaagcgcagg aagccctcct
    11581 cgaggttcac catgttcagc atgcccaggc tgttcaaaag ttcaatggtc cggtatatcg
    11641 tggctatccc tatggagggg tcctcccgct gggccagttc gagcaattcc ctggcgttca
    11701 ggtgtgtccc gaggtggcgc aggagcgtct ccacgatgat ccgcctctgg ttggtcagcc
    11761 tgaagcccag cgacttcagc cttcccatgt acatttcgat gctctcggtc agttcctcct
    11821 cacgcattca aggctccttt ccctctcggg aggcccgctc tcagcggaag atcagctcct
    11881 tgtccgaggg gtaggtcacg gtatacttca cgcttacctg gctgctcttc cccttgtcga
    11941 gggcgatctc ccaggtgagg aaacccttct cctccctctc ggcaggcgcc ggggaaagga
    12001 tcacgtcctg gaccttgatc gcctcgttgg ccggcacggg cacccggtcc ttcaccctta
    12061 ccttcacggc cttgtccagg ccgttgctca ccgttattat atacccccgc tggtgcttgc
    12121 ccttcacgat ccagctggtg cccgtggtgg ggatcatctc ctcccgcttc gccgtcacca
    12181 ggggcgtcgt cccgaagggc aataccgcct cctgccccat ggagagggcc tcgaggcgcg
    12241 tcctgcccgt gtccttgccg tcgacggaga gcctcgcctc gccgggcagc aaggcccttc
    12301 cgagggactt caccgtcgcc accgtccagg cttcaggcga gtactcgggg atacagacca
    12361 gcgagacctc acccttttcc gtgaaggtcc ccgcgtcgat ggtcacgtcc tcgccggaac
    12421 cgttgaccgc cgcgctggtc ctgatggcca cgtcggtgag cccctcctcg aggtactccc
    12481 cggcggcaac gtcctgagcg ggggcagccg ccctcatggc gagagcgaag ccctttgcgt
    12541 tcttggtcgg gtcatggata tcggctatca gcgggggcat atcggggata ctgaccccgc
    12601 ccctgggcct caccgtatgg aagaccacct cgccgtccca gtcgatgccg ctcttctggt
    12661 tcacctcgac gccgtagacg ccccggatct cccccgtcga actgtccagt tccagcctgt
    12721 agagcggcct ccaacgggcg ctgtcggtcc gggccgtcag caggacctgc cccttgccct
    12781 ccgtcgaaaa ggtcacgaag acgatcctgt cgggccggcc ggggaagcgt ccctcgtact
    12841 cggctttcag ggcctcgtgt tccttctggg ccttctccag gagccccttg gtctccgacg
    12901 ccttcagctc cagggcctct cttttcttca aagccgtatc tatgtaggcc gtcagctccg
    12961 cctctttcag tccctcgggc acggccgcct ccaggtgctt caccccctgg gcgatcgagg
    13021 cggccctgga ggagaggacc tccacctggg catgggcctt ttccatttcc tcggagaggg
    13081 tggccagcga tggcggcacc ca
//
LOCUS       DAOSRG010000014        12868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4887_length_12868_cov_8.719504, whole genome shotgun sequence.
ACCESSION   DAOSRG010000014 DAOSRG010000000
VERSION     DAOSRG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_12868_cov_8.719504"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLM30_01200"
     CDS             complement(<1..344)
                     /locus_tag="PLM30_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR53816.1"
                     /translation="MSNIGRIRRLAGTVIRADSAEPVSNFEVARVGSERLLGEVIRID
                     GKEVDIQVYEEIDGVRVGEPVEFTGEPLGIDLGPGLLGGVLDGIGRPLGGLSAAQADD
                     FFLKRGFFSRSL"
     gene            complement(350..625)
                     /locus_tag="PLM30_01205"
     CDS             complement(350..625)
                     /locus_tag="PLM30_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53817.1"
                     /translation="MAMQNYGNGYVLGRQPFVELWSLLGLVAVVCEGPEDFPACFQAL
                     QGEEVAFVLVESDWAESIPEFYKRKAKISDPVWVEMPSLKGSLKGRE"
     gene            complement(632..1678)
                     /locus_tag="PLM30_01210"
     CDS             complement(632..1678)
                     /locus_tag="PLM30_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOR53818.1"
                     /translation="MKSSKGFPENFGYLNARLRARFGDFLAREDYRRLASRSLEDIEV
                     FLLDTRYGKSFRRSLIEQSLSTHRKIEMALADTSASVLSHTRNMAMGEPEDLISVILS
                     RADLQNGRLLVRYFFIGIPYEMQPRWHAYGTLPVRFFAQLWKSPSVAHGIDKCLAFDH
                     PLARALARALEQVVKGSSLPASERALLSGMLECQERVLDSHRTKNSALVKEYLARTID
                     VWNLNIWFRVFSGVMQKEKAAELYLKGGFSLSPERLQGSPEPAGLLDSTPWENILDCS
                     KGGPPEIAPGGAMREFWRWQISLRRKDPLGIEVAIAFIAHQLVEWRNLDSLVVGVSMG
                     FEGDAILEKLILAG"
     gene            complement(1635..2273)
                     /locus_tag="PLM30_01215"
     CDS             complement(1635..2273)
                     /locus_tag="PLM30_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53819.1"
                     /translation="MLAAVTGIAEFKRALKEEFSKKSAALRGESESFISDRIFVARRE
                     TERKVHDIRAGHKKRFDLLLEREKRRTLLKVREEALGKISSILEKTTERVGLQIEAIR
                     GDREVYSGILSCLVREALEALEAEAVILVLPGEGGLVPEDERIRAVEESESVTSWGGC
                     LATDAPTRTLYIDNTIKTRWDLYQKELLRGLSERYRHVLEEFERFSREFRLS"
     gene            complement(2312..2530)
                     /locus_tag="PLM30_01220"
     CDS             complement(2312..2530)
                     /locus_tag="PLM30_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOR53820.1"
                     /translation="MEKALIALAAALAVAIPAMATAYAQARIGSVGAGTIAEKPETSG
                     IIIILEALPETMVILGFVVAVMLILQFA"
     gene            complement(2515..4422)
                     /locus_tag="PLM30_01225"
     CDS             complement(2515..4422)
                     /locus_tag="PLM30_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOR53821.1"
                     /translation="MIRDMLFFGLWGLVVDRPRIIGKLHDLGVVHLHEEPLEYVSPET
                     AAEMKILRGKALGLLESLGWSEWVSIPREVLEEERVLLSTSDETMIEAVEESLDSFSA
                     RLSGKLRMKADLEIKLNSIKGALRTISHFDTFIRDNARSGLEISIWWAQKASIPEPAA
                     RIRKIVRDKNPLEKGDRIPYLLRVTPGGERILAIAYPPEFREVVREILLSEQCIPWML
                     PGDYESMTFREALPLMEAALEEIPRGIDGIDRNLKAARREWGPKLGALYVILDEKVEE
                     ALVESGSETRGEVFRFSGWVPADEKDELAARLKTEFGERLFLRWREPQPGEWPEVPTS
                     LKNPRYFQPFELFLKLMPVISYKGLDPTVLIGLFFPLFGGCMVGDMGYGLVIGVLGFW
                     LRRKDGQPIMKDVGCILLYLAAWSIFWGAAYGEVFGDLGHRFFHLEPLWLERSQVVLP
                     VMAFTVGLGFAHVFLGFLMGVVQGLRTGQRHLWMERLGNAIVFLALLSVMVQVKGWLP
                     GRLFTIPVTLLVLGLVLLLAGGGMGGLVEALGSVGNVLSYVRIAAIGLSSAILATVAN
                     SFVDSLGVTFFGVFMALAIHLLNFALAVAGSSLHSARLQYVEFLGKFHAGGGTLYKPF
                     SRREPGLWKRH"
     gene            complement(4419..4781)
                     /locus_tag="PLM30_01230"
     CDS             complement(4419..4781)
                     /locus_tag="PLM30_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53822.1"
                     /translation="MKPGDHQVFEDPDAEVESFRQALEARLEEEKTLLEEEREGALKR
                     IEELEQRSLAEAEEEWNRYEKALITSTGSFAEDLRGEIISLLDPVLEKVLTEEFVEIA
                     VRAILPPAPRGSGGGRPF"
     gene            complement(4768..5235)
                     /locus_tag="PLM30_01235"
     CDS             complement(4768..5235)
                     /locus_tag="PLM30_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR53823.1"
                     /translation="MTINAGQLMHRDLSSVSEEDHVLDAVHLLYSQRLSGVPVVRDDW
                     VLQGYLSERDILRRATPTFLEVLAQSTFLDDSEGDLFERLKQIGDMKVKDVMNPDPIF
                     VKPTESLMTVVDLMLRKGLKRLPVVEGRKLVGVIERGGFCEFMMEGKEFNEAR"
     gene            complement(5240..6970)
                     /locus_tag="PLM30_01240"
     CDS             complement(5240..6970)
                     /locus_tag="PLM30_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOR53824.1"
                     /translation="MAAGVHELQGMVFLSLSIFVFFFAIQRISKVTLERDQYQYLKDV
                     CLLAAWGICGVWSPDPSVRLVVAMGLLSAILGVGQRVNPKMPWSLGFFGIGLVFASLG
                     LGISFVGFPGGSYLFLNRYSSLLATALWVGLFPLLLQEMDQVPGLAGFLSAVSWIVML
                     IVTGLSKQHIQGAFYMTLVGVSILAVFWSRHGHSYQRLGEPVAALWGTLIAGTSIMGV
                     SKGVTFSTLMLIPLGLFAIPMVEASVNIVSRTFSSRSQGSLFLYRRLVSRGMDHTPAV
                     RSVGSLCAVMGAAVASFQLRDENMALAALLSSTILLGGFLFVVSTGIKRSTSNRSSLW
                     GTPIDSISADYALGRTRSLILQGGPGRYFVTLDALSALRAREDRHFREIVTGADLVLP
                     DGKGLTWALGFLGAPSVQRIPGVEFVERMCRLASMEGWPVFLLGGAPGVGEKAAENLK
                     KLYPGLLIGGVRHGYLDASEAGSLLEEIRDSGSRFIFVGMGVPRQEAWMFEHHHLLPG
                     TISIGVGGSFDVISGNLKRAPLFLQKAGLEWLFRLIQEPWRWSRVRRLPLFVYHVILT
                     KFGFSHQKGE"
     gene            complement(7086..8360)
                     /gene="serS"
                     /locus_tag="PLM30_01245"
     CDS             complement(7086..8360)
                     /gene="serS"
                     /locus_tag="PLM30_01245"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOR53825.1"
                     /translation="MLDIKLFRERPEELSKALAARYGVFPVEEVVGLDARRREVLTLT
                     DDLRSERNQGSREIAELRKSGGDAAGLTERMRLVGEEIRKNEEMISDMENRIQALLLE
                     IPNIPHESVPVGEDENSNKVVRTWGEPRKFDFKPLPHWELGEALGIMDFERGVRLAES
                     RFTVLKGLGARLERALVNFMLDIHTNEHAYQEVQPPFMVNSETMKGTGQLPKFAEDLY
                     SCSSDDLWMIPTAEVPLTNLHRGEILPEDDLPLRYTAYTPCFRREAGSHGRDVRGMLR
                     QHQFDKVELVKICSPESSYAELESLTASAEEILKRLGLPFRTMLLSTGDMGFAASKTY
                     DLEVWLPSQDKYREISSCSNCEDFQARRMNTRFRPRDGGRVRYVHTLNGSGIAVGRCL
                     IAILENYQNRDGSVSIPGSLVPYMDGTEVIGK"
     gene            complement(8378..8848)
                     /gene="ribH"
                     /locus_tag="PLM30_01250"
     CDS             complement(8378..8848)
                     /gene="ribH"
                     /locus_tag="PLM30_01250"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOR53826.1"
                     /translation="MRTIQGKLVGTGLRFFLAVSRFNELISSRLLEGAKDFLLRHDVQ
                     PQDIDVAWVPGAWELPLAAREGALTGKYDAVIALGAIIRGDTPHFDFVASEVSKGLAM
                     VELEQRVPVAFGVLTCDTLEQALLRAGSKSGNKGADAAAAAIEMADLLSQLRKS"
     gene            complement(8893..>9996)
                     /gene="ribA"
                     /locus_tag="PLM30_01255"
     CDS             complement(8893..>9996)
                     /gene="ribA"
                     /locus_tag="PLM30_01255"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOR53827.1"
                     /translation="DAESINFMVSRGRGLVCVPMDEKVAKRLNLEPMVRENTDLHSTA
                     FTVSVDARDGVTTGISAAERALTARLLASPEATPEMFRRPGHMFPLIARCGGVLKRAG
                     HTEAAVDLARLAGRPPVGTICEIMKDDGSMARFPDLVEFAELHGLKIITIEELIRYRN
                     KKEKLVEKVAVVNLPTEYGDFVAHAYVSVLDESGDKVHLALVKGNISPERATLVRVHS
                     ECLTGDALGSLRCDCGPQLKTAMRMIEGEGNGVLLYMRQEGRGIGLLEKLHAYELQEK
                     GLDTVEANEALGYKPDLRDYGIGAQILSDLGLKRIRLITNNPRKIIGLEGHGLEVVDR
                     IPLVISPNAFNEKYLETKENKLGHMLHLKDIIG"
     assembly_gap    9997..10096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10097..10684)
                     /locus_tag="PLM30_01260"
     CDS             complement(<10097..10684)
                     /locus_tag="PLM30_01260"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOR53828.1"
                     /translation="MFTGLVECTARVTGLRRSGEVVELVIGGAPIDWELKRGDSVSVS
                     GACLSVVARERSGFTVQMMPETSKNTILGNVKPGYRVNLERAMPASGRLEGHIVTGHV
                     DLVSQVVGLVDLGRSRELDFSLPPEAARFVARKGSIAVQGVSLTVISSEAGQFRVGLI
                     PETLADTTLGDLRIGDPVNVEYDILSKYLVNLLEKR"
     gene            complement(10648..11772)
                     /gene="ribD"
                     /locus_tag="PLM30_01265"
     CDS             complement(10648..11772)
                     /gene="ribD"
                     /locus_tag="PLM30_01265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOR53829.1"
                     /translation="MMMNDPKRDALNMRAALSLALRGTGRVSPNPLVGCVIVGPGGLV
                     GWGYHGEVGGPHAEAVALDRAGERARGATLFVNLEPCRHHGRTPPCAPRIIRAGIARV
                     VAGMADPDPRTAGGGFAMMREAGVEVTQGVLEYRCRFINRGFLSRVERKRPWVTLKMA
                     ATLDGNMAMPSGESRWITNSFSRVRAHLLRDANDAVMVGIGTVSADDPLLTVRDTDGR
                     DPIPVVLDPSLRMPPSARLARDGTIIFYSREAPAKTLEALRAKGCILYEVEADRTGRP
                     SLESVLEKLAHQGVNNLLIEGGPGVLGSFLAGRLFDGLALFLAPRFSGKGHGIGDGFV
                     LDRLADAIGVRVESIRDLDGDLWLEGVNPCSQDWLSARRG"
     gene            complement(11759..>12868)
                     /locus_tag="PLM30_01270"
     CDS             complement(11759..>12868)
                     /locus_tag="PLM30_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR53830.1"
                     /translation="EGDGADYIGPFVSAGELRQMLRLVERFFPLRICRTDSRGGKWAA
                     RPCVRYDLGRCLGPCAGLADPSEYRERVADIALLLRGQSASLVGRLRERMDKASASMD
                     FETAGRYRDTIRAIWRLSRRHVSMVFSDPLDGGIWKSLTQLQEVLSLPTLPWRIEGYD
                     ISHFAGGEAYGVCVVFEQGLPNPSLYRRYRIRTVEGIDDFRSIAEVLERRYKRVLEES
                     GVFPQLILIDGGPGQLSFAVEALKALGLADVPVAAIAKKEELLFVPGRVKPIRLPREN
                     EALMLLQRVRDESHRFAIAANRRAGRKRMSRSSLEDVPGIGKILASRLLSRFGSVARI
                     STLTPEEISSVKGVSAEVAKRIIRHLGGREQDDDE"
BASE COUNT         2570 a         4143 c         3590 g         2465 t
ORIGIN      
        1 tcaaggcttc tcgagaaaaa acccctcttg aggaaaaagt catcggcctg cgcggcagaa
       61 aggccgccga gcggtcggcc tataccatcg aggacgccgc ccagcagtcc tgggcccagg
      121 tcgattccca gcggctcccc ggtgaattcg acgggttcac cgacgcggac gccgtcgatc
      181 tcttcgtaga cctggatatc cacttccttt ccgtctatcc tgatcacttc tcccaagagc
      241 cgttccgacc ccaccctcgc gacctcgaag ttggagaccg gctcggccga atccgcccgg
      301 atcaccgttc ccgcaaggcg cctgatcctt ccaatgtttg acatgaccct cactcccgcc
      361 ctttaagact gcccttcagg gagggcattt ccacccagac agggtccgag atcttcgctt
      421 tcctcttgta gaactccggg atgctctccg cccagtccga ctcgaccagg acaaaggcca
      481 cctcttcacc ctggagtgcc tggaagcacg cggggaaatc ctccggcccc tcgcagacca
      541 cggccacgag accgaggagg gaccagagtt ctacaaaggg ttgcctgccg agaacgtacc
      601 cgttgccgta attctgcatc gccatcgacg gtcaccccgc aaggatcagc ttttccaaaa
      661 tggcatcccc ttcgaacccc atggacaccc ccaccaccag tgagtccagg ttccgccatt
      721 ccaccaattg gtgagcgatg aaagctatcg ccacctcgat acccagggga tcctttcttc
      781 tcagggagat ctgccaccgc cagaactccc gcatagcccc tcccggggct atctccggtg
      841 ggccaccctt ggaacagtcc aggatattct cccagggcgt tgagtcgagc agcccggcag
      901 gctcggggga gccctggagc cgctccgggg acagggaaaa accccccttc aggtagagct
      961 cagcggcctt ttccttctgc atgacgccgg agaaaaccct gaaccagata ttcaggttcc
     1021 acacatcgat ggtcctggcg aggtattcct tcaccagggc cgaattcttc gtcctgtggc
     1081 tgtccagcac cctctcctgg cattccagca tgcccgacag gagcgccctt tcgctcgccg
     1141 ggaggctgga tcccttcact acctgttcga gcgccctggc aagagccctc gccagggggt
     1201 ggtcaaaggc aaggcacttg tcgatcccgt gggcgacgga cgggctcttc catagctggg
     1261 cgaagaagcg cacggggagg gtaccgtagg cgtgccaccg gggctgcatc tcgtagggga
     1321 tgccgataaa gaagtaccgg actagaaggc gcccgttctg aaggtccgcc ctggacagga
     1381 tcacgctgat gagatcctcg ggttcaccca tggccatatt cctggtgtgg ctcaagaccg
     1441 atgccgaggt gtccgcgagg gccatctcga tctttcggtg ggttgacagg gactgttcga
     1501 tcaggctccg cctgaaactc ttgccgtacc tggtatccaa aaggaagacc tcgatatcct
     1561 cgaggctccg ggatgccaac cttcggtaat cctctcgggc caggaagtcc ccgaaacggg
     1621 cacggagcct ggcgttaaga tagccgaaat tctctggaaa acctttcgaa ctcttcaaga
     1681 acatgtcggt agcgctccga aaggccgcga aggagctcct tctggtaaag gtcccacctg
     1741 gtctttatcg tgttgtctat atacagggta cgggtcgggg cgtccgtcgc caggcaacct
     1801 ccccaggacg tgacggactc gctctcctcc acggccctta tcctttcatc ctcgggcacc
     1861 agccctccct ccccggggag aacgaggatc acggcttcgg cctcaagtgc ctcaagggcc
     1921 tccctgacaa ggcaggaaag gatacccgag taaacttcgc gatcaccacg gatggcctcg
     1981 atctgaagtc cgaccctctc cgtggtcttc tccaggatgg aagagatctt gcccagcgcc
     2041 tcttccctca ccttgagcag ggtgcgcctc ttctcccttt caaggagaag gtcaaaccgc
     2101 tttttgtgcc ctgcccttat gtcatggacc tttctttcgg tctcccttct cgccacgaag
     2161 atcctgtccg aaatgaagct ttccgattcg ccgcggagcg ccgctgactt tttggaaaac
     2221 tcctccttca gcgctctttt gaattccgcg atccccgtca cagcggctag caccccctcg
     2281 gtaccatcgg ggttcccagg tcacggaagg atcaggcgaa ctggaggatc aacatgacgg
     2341 ccacgacaaa acccagtatc accatcgtct cgggaagggc ttcgagtatg atgataattc
     2401 cgcttgtctc gggcttttcg gcgatagtcc cggctccgac gctgccgatc ctcgcctggg
     2461 cgtaagctgt agccatggca gggatggcca cggccagggc tgccgcaaga gcgatcaatg
     2521 ccttttccat aatcccggct ccctccttga aaagggttta tacaaggttc cccccccggc
     2581 atggaacttc ccgaggaatt ccacgtactg gagtcgcgcc gaatgcaggc tcgaacccgc
     2641 aacggcaagg gcgaagttca acaggtgtat cgccagggcc atgaataccc cgaaaaaggt
     2701 gacccccagg gaatccacga agctgttggc caccgtggcc agtatcgcgg aagagagtcc
     2761 gatggccgcg atacggacgt aactcaggac attccccacc gatccgaggg cttcgacaag
     2821 gccgcccatg ccgccaccgg caagaagcag gacgagcccc agcaccaaca gcgtaacggg
     2881 gatggtgaac agccttcccg gcagccaccc cttcacctgg accatgaccg aaagcagggc
     2941 caggaaaacg atcgcattgc ccagtctttc catccagagg tgtctttgtc ccgtcctcaa
     3001 cccctggacg acccccatga ggaagcccag gaagacgtgg gcaaaaccca gacctaccgt
     3061 gaaggccatc accggcagta caacctggga tctctcaagc cagaggggct ccaggtggaa
     3121 aaaacggtga cccaggtcac cgaagacctc tccataagcg gccccccaga agatggacca
     3181 cgcggcgagg taaaggagga tgcagccgac gtccttcatg atgggttggc cgtccttccg
     3241 cctgagccaa aaccccagga cgccgatgac caggccgtag cccatgtcgc ccaccatgca
     3301 gcccccgaaa agggggaaaa acaacccgat gagcacggta gggtcaaggc ccttgtagga
     3361 aatgacgggc atgagcttca ggaaaagttc aaagggctga aaatacctgg gattttttag
     3421 agaggtgggc acttcgggcc attcgcccgg ctggggctcc ctccagcgga gaaaaagcct
     3481 ctccccgaac tcggtcttca accgcgccgc cagctcgtcc ttctcgtcgg ccgggaccca
     3541 cccggaaaac ctgaagacct cacccctggt ctcgcttccg gactcaacga gagcctcctc
     3601 gaccttttcg tccaggatca cataaagagc tcctaacttg ggcccccatt ccctccttgc
     3661 agccttgagg ttacggtcga tcccatctat accccggggg atctcttcca gggctgcctc
     3721 catgagaggg agggcttcac gaaaagtcat gctctcgtaa tctcccggga gcatccaggg
     3781 gatgcattgt tccgaaagga gtatctcccg gaccacctcg cgaaattcag ggggataagc
     3841 tatagcaagg atcctctccc cccccggggt gacccggagg aggtagggga tcctatcacc
     3901 cttttccagg gggttcttat ccctaacgat cttccggatc ctggcggcag gttcaggaat
     3961 actggccttc tgggcccacc agatcgagat ctcaaggccc gacctcgcgt tgtcacggat
     4021 gaaggtgtcg aagtgcgaga tggtcctgag ggcgcctttt attgaattga gctttatctc
     4081 caggtcggcc ttcatccgca acttgccgga gagcctggca ctgaaggagt ctaggctctc
     4141 ctcgacggct tcgatcatgg tttcgtccga ggtcgagagc agcactcttt cctcctctaa
     4201 aacttcccgg gggatcgaaa cccattcact ccagccaagg ctctcgagca gccccagggc
     4261 cttgccccgg aggatcttca tctccgccgc cgtttccggt gatacgtatt ccagcggctc
     4321 ttcgtgaagg tggaccacac ccaggtcatg gagctttccg atgatccggg gcctgtcgac
     4381 gacgagaccc cagagcccga aaaagagcat gtcccttatc aaaagggccg ccctcctccc
     4441 gagcccctgg gggcgggggg aaggatcgcc ctgacggcga tctcaacaaa ttcttcggtc
     4501 aacacctttt ccaggaccgg gtcaaggagg gagattattt cgccccggag gtcctcggcg
     4561 aaggaaccag tagacgtgat aagcgccttt tcgtaacggt tccattcttc ttcggcctca
     4621 gcgagggacc gttgctcaag ctcttctatc cttttcagcg ccccttcgcg ttcttcctcg
     4681 aggagtgtct tctcctcttc cagccttgcc tccagggcct gcctgaagga ctcgacctcg
     4741 gcgtcggggt cttcaaaaac ctggtgatca cctggcttca ttgaattcct tgccttccat
     4801 catgaattcg cagaaaccgc cccgctcgat cactccgacc agtttgcggc cctccacaac
     4861 ggggagcctc ttcaaaccct tcctgagcat caggtccacg acggtcataa ggctctccgt
     4921 cggcttgacg aagatggggt cggggttcat cacgtccttg accttcatgt cgcctatctg
     4981 tttcagcctt tcgaagaggt cgccctcgga gtcgtcgagg aaggtgctct gggcaagcac
     5041 ttccaggaag gtgggcgttg ccctgcgaag gatgtccctc tccgagaggt atccctggag
     5101 cacccagtcg tcccttacga cgggaacacc ggaaagtcgc tggctgtaaa gcaaatgcac
     5161 agcgtcaagt acatgatctt cctccgatac ggatgaaagg tccctgtgca tgagctgccc
     5221 ggcgttgatg gtcaaaacat tattcccctt tctgatggga aaacccgaat ttcgtgagta
     5281 tcacgtggta gacgaaaaga gggaggcggc gcacccggct ccaccgccag ggttcctgga
     5341 tgagcctgaa aagccactcg agccccgcct tctgcaagaa aaggggcgcc cttttaaggt
     5401 tgccggagat gacgtcgaag ctgccaccca cgccgatgga aatggtaccc ggcagcaggt
     5461 ggtgatgttc gaacatccag gcctcctgcc tcggtacccc catcccaacg aagatgaaac
     5521 gcgatccgga atccctgatc tcctccagca ggctccccgc ttcggaagca tcgaggtagc
     5581 cgtgtcttac gccaccgatc aaaaggccgg gataaagctt tttcaggttt tcggcggcct
     5641 tttcgcctac tcccggggcg cctccgagga gaaaaaccgg ccagccctcc atcgaggcta
     5701 aacggcacat tcgctcgaca aattcgacgc cgggtatcct ttggaccgaa ggagccccca
     5761 ggaaacccag ggcccaggtc aagcccttgc cgtcgggaag gaccaggtcg gcgccggtca
     5821 cgatctccct gaaatgcctg tcctccctgg ctctcagcgc ggagagggcg tcaagggtca
     5881 caaaataacg gcccgggcca ccttggagaa tcagcgaccg ggtcctgccc agggcgtaat
     5941 cggctgaaat actgtcgata ggcgtccccc agagggagga acggttcgaa gtggatctct
     6001 ttattcctgt ggagacgacg aaaaggaaac cgccgagcag gatcgtcgac gaaaggaggg
     6061 ctgccagggc catattctcg tccctcagct ggaagctcgc cacggcggca cccatgaccg
     6121 cgcataacga acccaccgaa cggacggccg gcgtatgatc catgcccctg ctcacaagcc
     6181 tcctgtaaag gaagagggag ccttgggaac gggaggaaaa ggtccggctg acgatattga
     6241 cggaggcctc taccatgggt atggcgaaaa ggccgagagg gatgagcatg agggtggaaa
     6301 aggtcacacc cttgctgacg cccataatgg aagtaccggc gatcagggtg ccccagaggg
     6361 ccgccacggg ctcgccgagg cgttgatagg agtgaccgtg acggctccag aaaacggcca
     6421 gtatggagac acccacaagg gtcatataaa aagcaccctg gatatgctgc ttggaaaggc
     6481 cggtgacgat gagcatcact atccaggata cggcggagag gaagcccgcc aggccgggaa
     6541 cctggtccat ctcctgcaaa aggaggggga acaggccgac ccaaagcgcc gtggccagca
     6601 gcgaggagta ccggttgagg aagaggtaac tgccaccggg gaatccaacg aaggatatgc
     6661 ccaaccccag ggaggcgaag acaaggccta tgccgaagaa acccagggac catggcatct
     6721 tcgggttgac cctctggccc acgccgagga tggccgacaa gagacccatg gccaccacga
     6781 gccggaccga ggggtcgggt gaccagaccc cgcatatccc ccacgccgcc aggaggcaga
     6841 catccttaag gtactggtac tggtctctct ccagggtcac cttcgatatc ctctggatcg
     6901 cgaaaaagaa gacgaagatc gaaagcgaca gaaacaccat accctgaagc tcatgaacac
     6961 cagcggccat aaaatgccct ccccgatgga ataaccctca tcgccgtcaa aaaccgcttc
     7021 tgaggccagg agcagcattc cgtcatgatt ttagcatgaa aagagcccga tgatgaaaaa
     7081 taagtctatt ttccgatgac ctcggtccca tccatgtagg ggaccagact tcccggtatc
     7141 gataccgacc cgtcacggtt ctggtagttc tcaaggatgg cgataaggca tcgtccgacg
     7201 gctatccccg agccgttgag ggtatggaca taccgcactc tgccgccatc cctggggcgg
     7261 aacctcgtgt tcatcctgcg agcctggaaa tcctcgcaat tactgcagga actgatctcc
     7321 ctgtacttgt cctgcgaagg gagccacact tccaggtcat aggtcttgct ggcggcgaag
     7381 cccatgtccc ccgtcgacag gagcatcgta cggaagggga gcccaagcct tttaaggatc
     7441 tcctccgcgg aggccgtcag tgactccagt tcggcgtagc tgctctcggg agaacagatc
     7501 ttgacaagtt ccaccttgtc gaactggtgc tggcgaagca tcccgcggac gtcacggccg
     7561 tggcttcccg cctcccgtct gaagcagggc gtataggcgg tgtagcgcag gggaaggtcg
     7621 tcctcgggta gtatctctcc ccgatggagg ttcgtcagcg gcacctcggc ggtgggtatc
     7681 atccagagat catcggaaga acaggagtaa agatcctcgg cgaatttcgg caactgcccc
     7741 gtccctttca tggtctcgct gttcaccatg aagggcggtt gcacctcctg gtaggcgtgt
     7801 tcgttagtat gaatatcgag catgaagttg accagggccc tctcgagtct ggcccccaga
     7861 cccttgagga ctgtgaaccg gctctcagcg aggcgcaccc ctctctcaaa atccattatc
     7921 cccagcgctt cccccagttc ccaatgaggt aaaggcttaa aatcgaactt ccggggttcg
     7981 ccccaggtgc ggaccacctt gttggaattc tcgtcctccc ccaccgggac cgactcgtgg
     8041 gggatatttg gaatctcgag gagcagggcc tggatgcggt tctccatgtc ggagatcatc
     8101 tcttcatttt tcctgatctc ctcgccgacg agtcgcatcc tctcggtcag ccccgcagca
     8161 tcgccgccgc ttttcctcaa ctcggcgatc tccctggagc cctggttcct ttcggacctc
     8221 agatcgtcgg tgagggtgag cacttccctg cgccgggcgt ccagcccgac gacctcctca
     8281 acgggaaaaa cgccgtacct cgctgccagg gccttgctca gttcctcggg tctttcacga
     8341 aaaagtttga tgtcgagcat ggatatcctc caaactgtca ggattttctc aactggctca
     8401 aaaggtcggc catctcgatg gcggcggcgg cggcatcggc acccttgttg ccggacttgc
     8461 tcccggccct caggagcgcc tgttcgaggg tatcgcaggt caggacgccg aacgccacgg
     8521 ggaccctctg ctcgagttcc accatggcga ggcccttgga tacctcggaa gccacgaaat
     8581 cgaagtgagg cgtatcaccc ctgatgatcg cacccagggc gatcacggcg tcgtacttgc
     8641 cggtgagggc tccctccctg gctgccagtg ggagctccca ggcccccggg acccaggcga
     8701 catcgatatc ctggggctgc acatcgtgcc tcaaaaggaa atccttggcc ccttccagga
     8761 gcctcgagga gataagttcg ttgaaccggg agactgcaag gaagaaccgc aggccggtac
     8821 ccacgagttt gccctggatc gtcctcatag cgtatcactt cccccttttc gttttatagc
     8881 cccaccggcg gtctagccga tgatgtcctt cagatggagc atgtggccca gtttattctc
     8941 cttggtctca aggtactttt cgttgaaagc gttgggggag atgacaaggg ggatcctgtc
     9001 caccacttca agaccgtgcc cctcgagacc tatgatcttc ctggggttgt tcgtgatgag
     9061 cctgatcctt ttcaagccga ggtcggagag tatctgggcg ccgatcccgt agtccctgag
     9121 gtcgggcttg tagccgaggg cctcgttggc ctccaccgta tcgagcccct tctcctgcag
     9181 ttcgtaggcg tggagtttct ccagcaaccc tatcccgcga ccctcctgcc tcatgtacag
     9241 caaaaccccg tttccctcac cctcgatcat cctcatggcg gttttcagct gcggcccgca
     9301 gtcgcaccgg agggagccca gggcatcccc cgtcaggcat tcggaatgaa ccctcaccag
     9361 ggtcgccctt tccggggaga tattcccctt caccagggcc aggtgcacct tgtcaccgct
     9421 ctcatccagc acggatacgt aagcgtgggc gacaaaatcg ccgtattccg tcggcaggtt
     9481 cacgaccgcg accttctcca ccagtttttc cttcttgttc ctgtagcgga tgagctcttc
     9541 gatggtgatg atcttgagcc catgaagttc ggcgaattcc accaggtcgg ggaaccgggc
     9601 catggacccg tcgtccttca tgatctcgca tatggtgccc accggcggcc tcccggccag
     9661 cctggccagg tccacggcgg cctcagtgtg accggccctt ttcaggaccc ccccgcaccg
     9721 tgcgatcagg gggaacatgt gccccggcct tcggaacatc tccggcgtcg cctcgggaga
     9781 ggccaggagc ctcgccgtga gagccctctc ggcggccgat atgcccgtgg tgacgccatc
     9841 ccgggcgtcg acgctcacgg tgaaggccgt agagtgcagg tcggtgttct ccctgaccat
     9901 gggctcaagg ttcagcctct tcgcgacttt ttcatccatg gggacgcaaa cgaggcccct
     9961 tccccgggag accatgaagt ttatgctctc cgcgtcnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnncctc ttttcgagaa gattcaccag gtattttgat agtatatcgt
    10141 actccacgtt caccgggtcg ccgatccgga gatcccccag cgtcgtgtcg gccagggtct
    10201 cgggtatgag tccgacccgg aactgtccgg cttcgctgga gatgaccgtc aggctgaccc
    10261 cctggacggc aatggaaccc tttcttgcta caaagcgcgc ggcctcgggg gggaggctga
    10321 agtccagttc cctgctccgc cccaggtcca cgaggccgac cacctgagag accaggtcca
    10381 cgtggccggt gacgatatgc ccctcgagcc gtcccgaggc gggcattgcc ctctccaggt
    10441 tgacgcggta gccgggcttg acgttcccca ggatagtatt cttggaggtt tcgggcatca
    10501 tctgcaccgt aaaaccgctc ctctccctgg ccaccacgga aaggcacgcc ccggagacgg
    10561 agacggaatc gccccttttc aactcccagt cgatgggggc tccccctata accagctcga
    10621 cgacctcacc gctccttcta aggccggtta ccctcgccgt gcactcaacc agtcctgtga
    10681 acatgggttg accccctcca gccaaaggtc gccgtcgagg tccctaatgc tctcgaccct
    10741 gaccccgatg gcatcggcga gtctatcaag gacgaaacca tcgccgatgc cgtgcccttt
    10801 cccggaaaaa cgcggcgcga ggaaaagagc aagcccatca aaaagcctcc ccgccaggaa
    10861 tgaacccagt actccgggcc cgccctcgat aagcaggttg ttgaccccct ggtgggcgag
    10921 tttttccagc acgctctcca gtgagggcct tcccgtcctg tcggcctcaa cctcgtaaag
    10981 tatacaacct tttgcccgca aagcctccag tgttttggcc ggcgcttcac gggagtaaaa
    11041 gatgatcgtc ccatccctgg ccaggcgtgc cgatgggggc atcctcagcg aagggtccag
    11101 gaccaccggt atcgggtccc tcccgtcggt gtccctgacg gtcagcaggg ggtcgtcggc
    11161 ggatacggta ccgataccca ccatgacggc atcgttggca tccctcagca ggtgggccct
    11221 caccctggaa aaggagttgg tgatccagcg gctctcgccg gaaggcatgg ccatgttccc
    11281 atcgagggtc gctgccatct tgagggtaac ccaggggcgc ttccgctcga ccctcgagag
    11341 gaaaccacgg ttgataaacc tgcacctata ctccaggaca ccctgggtga cctccacccc
    11401 cgcctccctc atcatagcga agccgccgcc ggccgtcctg gggtcggggt cggccattcc
    11461 cgcgacgacc ctggcgatgc ccgccctgat gatcctgggg gcgcaggggg gagttctccc
    11521 gtgatgacgg cagggctcga ggttgacgaa aagcgtcgcc ccccgcgccc tctcaccggc
    11581 gcggtccagc gcgaccgcct cagcgtgggg tccccctacc tcgccgtgat aaccccaacc
    11641 cacgagaccg ccgggaccaa cgatcacgca ccccaccagg gggttggggc tgaccctgcc
    11701 cgtccccctg agggcgaggc tcagggccgc cctcatattc aaggcatccc ttttcgggtc
    11761 attcatcatc atcctgctcc cggcctccca ggtggcgaat tatcctcttc gccacctcgg
    11821 cgctgacgcc cttcacggaa gagatctctt ccggggtcag cgtggatatc ctggcgacac
    11881 tgccaaacct agaaaggagc ctcgaggcta ggatcttgcc tatgcccggc acatcctcca
    11941 gcgacgagcg ggacatcctc ttcctgccgg ccctcctgtt ggcggcgatg gcaaagcgat
    12001 gagactcgtc ccttacccgc tggaggagca tcagggcctc gttctcccgg ggcaggcgga
    12061 tcggtttcac cctcccgggg acgaaaagca actcctcctt tttcgcgatg gcggccaccg
    12121 ggacatcggc aaggcccagg gccttgagcg cctccaccgc gaaggagagc tgcccgggcc
    12181 cgccgtcgat gaggatgagt tgcgggaaaa cccccgactc ctccaggacc cttttgtacc
    12241 ttctctcaag gacctccgcg atggacctga agtcatcgat cccctcgacg gtgcggatcc
    12301 ggtacctcct gtacagggac gggttcggga ggccctgctc gaagacaacg cagacgccgt
    12361 aggcctctcc gccggcgaag tgggagatat cgtacccctc gatcctccag gggagggtcg
    12421 ggagggacag cacctcctgg agttgggtga ggctcttcca gataccgcca tcgagggggt
    12481 ccgagaagac catggacacg tgcctcctgg acagcctcca gatggccctt atggtgtccc
    12541 tgtacctgcc ggcggtctcg aagtccatcg aggccgaagc cttgtccatc ctctccctca
    12601 acctacctac gagggaggcg gactgacccc tcaggagcag ggcgatatcg gccaccctct
    12661 cccggtactc cgaagggtcg gcaagcccgg cacagggccc aaggcacctc ccgaggtcgt
    12721 acctcacgca gggcctggcg gcccacttgc cgcccctgga gtcggtcctg catatcctca
    12781 aggggaaaaa cctctccact agccggagca tctgccgcag ttcgccggcg ctcacgaagg
    12841 gccctatgta gtccgcgccg tccccctc
//
LOCUS       DAOSRG010000015        12790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4926_length_12790_cov_9.236121, whole genome shotgun sequence.
ACCESSION   DAOSRG010000015 DAOSRG010000000
VERSION     DAOSRG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_12790_cov_9.236121"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLM30_01275"
     CDS             complement(<1..331)
                     /locus_tag="PLM30_01275"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300397.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR53831.1"
                     /translation="MKSVMMDADPEIDSLVQSEFLRQKQSLELIASENFVPPYVLQAQ
                     GSVLTNKYAEGYPHKKYYGGCEFVESAEELAAKRACELFGAEHANVQPHSGTQANMGV
                     YFTVLEPG"
     gene            complement(420..>1925)
                     /gene="thrS"
                     /locus_tag="PLM30_01280"
     CDS             complement(420..>1925)
                     /gene="thrS"
                     /locus_tag="PLM30_01280"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOR53832.1"
                     /translation="PREQALALFRERGAVYKLELVSEIPDEFISIYRQGEFADLCRGP
                     HVVSTSRLGHFKLLSVAGAYWRGDEKNIMLTRVYGTAFDTAEALQDHINRIEEAKRRD
                     HRKLGKELDLFSIQEEGPGFPFFHPKGTVVINRLVEFWRAEHARRGYSEIRTPLILDQ
                     ELWVRSGHWDHYRDNMYFTEIDGRPFAVKPMNCPGGILVYKSRIRSYRDFPMRVAELG
                     TVHRHERSGVLHGLMRVRSFTQDDAHLYVTPGQIKQEIIGIMDLVRFIYGDVFGFSYR
                     VELSTRPEKAMGDPLMWEKAERALQEALEEFGVPFRLNPGDGAFYGPKIDFHLEDCIG
                     RTWQCGTIQLDFQMPEKFDMTYIGPDGNEHRPVMLHRTILGSLERFMGILIEHYAGAF
                     PFWLSPIQVRIVPVKEEHSPYASEVESRLKGLGYRVETDARDEKLGKKIRDAQVEKIP
                     YMVVIGSREVENVTLSVRDRGEGDLGTMDLSAFEALLQRQFDPVLGRGSIA"
     assembly_gap    1926..2025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2603..3043)
                     /gene="dut"
                     /locus_tag="PLM30_01285"
     CDS             complement(2603..3043)
                     /gene="dut"
                     /locus_tag="PLM30_01285"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR53833.1"
                     /translation="MPGTVRVGIFHDDEGMTLPVYATALSSGVDLRSNEHAMIPPGGR
                     LSVGTGIRLEIPEGYECQVRPRSGLALKHGVTVLNTPGTIDADYRGEVRVILVNLGEN
                     PFEVERGDRIAQMVLAPVSRIEWTEKDLLSETGRGSGGFGSTGK"
     gene            complement(3046..5355)
                     /locus_tag="PLM30_01290"
     CDS             complement(3046..5355)
                     /locus_tag="PLM30_01290"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR53834.1"
                     /translation="MMETFSVEVGGRTMTFETGKLAKQANGAVRVTQGETSLLVTACI
                     SDKPREGLDFLPLLVDYEERFYSAGKIPGGFIKREGRPSETAILASRIIDRSIRSLFH
                     DHTRNDIHVVATTLSVDQSTPPNILAINGASAALMLSGIPWKGPVGAVRVGRIDGRLV
                     INPTEDLMKESTLDLVVSGHSGGITMVEAGAGEIPEDVMVDALEMAQKEIANLVQAQI
                     EMVAKAGKEPVKLPEAASLPEIDEWVERELYEEIFQAVQVHEKKPRGEAIDIATGKAL
                     ESLLEKYSEAEPYIKASVEGMVKKAMRSLIVDEGRRADGRAMDEVRPISCEVGFLPRV
                     HGSALFTRGETQALVTTTLGMVGEDDQLIDGLKLDEPSKRFMLHYNFPPFSVGEVRPM
                     RGPGRREIGHGALAERAVRGLLPGEEEFPYAVRVVSDILESNGSSSMASVCGASLSLM
                     DAGVPVKKAVAGVAMGLIKEGDKVVVLTDIQGLEDHYGDMDFKVTGTREGITALQMDN
                     KAGGITRAILEKALDQARRGRLFILDKMEAVIAEPRKNISAYAPRIYTLTVPLDKIRD
                     IIGPGGKTIRNIVAQTGVKVDVEDDGRVFVASPTTEAAEKALDMINDLVREVMAGEVF
                     SGKVTRLMAFGAFVEVLPGKEGLLHVSEISTTHVGKVDDVLSPGDSVLVMVKEIDDMG
                     RINLSRRRALERRNEPELAEKFADALVLEDAREERIKALPPSSDRDRGPRGGTPRPGG
                     RPGGDRPSGDRRPHGGPRRKDFGDRGRER"
     gene            complement(5489..5755)
                     /gene="rpsO"
                     /locus_tag="PLM30_01295"
     CDS             complement(5489..5755)
                     /gene="rpsO"
                     /locus_tag="PLM30_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR53835.1"
                     /translation="MLEKEKKQGIIEEFKTHNADTGSPEVQIAILTTRIRDLTEHLKV
                     HKKDHHSQRGLLKMVGRRRRLLQYLRNKDFGRYSDLIQKLGLRH"
     gene            6016..6696
                     /locus_tag="PLM30_01300"
     CDS             6016..6696
                     /locus_tag="PLM30_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR53836.1"
                     /translation="MNILIVEDNRDLVQVLTEGFNELEFSVDSAFDGIEGLNKIRKND
                     YDCVVLDIMLPEMDGYELIGRMREEGKDTPVIMLTAKDSIEDRVEGLNRGADDYLVKP
                     FDFRELVARVNAVTRRRTEPKRTVLHCGPLMLDPIARECKAEGEMVPLRRREFDILEL
                     LMRFENQVFSREKIIGLVWKKEYDGTSNVVDVHVKYLRDKLRPYNLDTVVVTVRGVGY
                     KVTCPDYE"
     gene            6816..8219
                     /locus_tag="PLM30_01305"
     CDS             6816..8219
                     /locus_tag="PLM30_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR53837.1"
                     /translation="MTIMVTIALFGMAVFTFFTSFEVSRLDAVLASSMEETVSFYEKS
                     GRFPLQVKGSRTLGVVLQVYNQDKSLMFHLPPGLSAPFIPEELLEKASFGVRPLTFVP
                     SKNSFLPRFWWVLPWNCLSDRDLWRALVATAPFRDEEVVLVSMAPMAWLLESRQLLLW
                     MTILAGAAGLIASIAAGSLLAAGAMRPIKAINKALAGVSIDNMSIDPSPFGSDREIGE
                     IVTHINRMLKNLEKSMKNLQQFTSDASHELRTPLAVMRGVVDIALLKQRDPEYYVQKL
                     QELTYHIDDMQNLVGALLELARLDDFRGLDSREPVDLLLVAEDAVGNMESITQRRGQV
                     VEKDLKPAPTLGRYVMVLRLANNLLENASKYSPPGSRIKISTYSDIGKNQAILEVRDR
                     GSGLDPEEISRCFDRFWRADSSRTTPDYGLGLPLVKRFALIHNASIEVESQKGKGSLF
                     RVRFPLDKETLEGYDLE"
     gene            complement(8269..9678)
                     /locus_tag="PLM30_01310"
     CDS             complement(8269..9678)
                     /locus_tag="PLM30_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOR53838.1"
                     /translation="MNRYKGSKILMATLLLLLSAAVVFSLPPGPAVAQDVFTGNPIAK
                     IVKETAPAVVNIDTEAIVQRSSSPFPDDPFFREFFGDRFERFTQDVPMRGKGSGFIVT
                     HDGYILTNNHVVDGPDNMTIEVTLSDGRSFPAKIVGKDPSYDLAVIKIDAEGLPTVPM
                     GDSDKVDVGEWVIAIGNPYGFESSVTVGVVSAKNRSVRARNLNFDGFIQTDAAINPGN
                     SGGPLLDLSGRVIGINTAIVPYAQGIGFAVPVNMAKQIVDDLIQYGKVNRGWLGVYLQ
                     PLTKEFAGAYGIKTGEGALVADVVKGSPAEKAGVARGDVILDIDGRKVKDPGDLVVAI
                     RQHLAGEKVELTVLSKGVEGKRMVTLSTVPSDGNETPARSEPVSSSKLGISVSPVTPE
                     LRQKYGMEGSGGAVITGVLSGSVGQRMGLREGDVVLEVNGETVKDVTQLEDILSRDLK
                     NIAMLVFRDGRTFYVSANL"
     gene            complement(9719..10537)
                     /locus_tag="PLM30_01315"
     CDS             complement(9719..10537)
                     /locus_tag="PLM30_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOR53839.1"
                     /translation="MTGEFRCVTFLSDFGLADETVACCKGLLLRRGVTLPVIDISHEV
                     PPFDIISGGWMLAGAVLYMPSGIHLAIVDPGVGTQRRILLLRSGRGDLFVGPDNGLLI
                     PAAERTGGISRCWSIERAQGEMEHVFPTFDARDIMAPVVADLVGGKPPEECGEEIPLG
                     ELNRFPLPAPHASPGKISGHVTLVDAFGTVRTDIPWDLLGKLGDSEIKISSGWLFSIP
                     VGRTFSDVGSGEMVALEDSWGFVSIAVNRGRASDMLGFKPGDPISVELTIPGRP"
     gene            complement(10609..11271)
                     /locus_tag="PLM30_01320"
     CDS             complement(10609..11271)
                     /locus_tag="PLM30_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOR53840.1"
                     /translation="MKGVNTVEDHETFEVDVNSLDEARHIGAIRWSVDPSEIEVHVLD
                     EGKRLFGILGKKMHVALSLRSQGPDGLPAGTFPFLEGLLERMDLSVEPEVTGPDSIDL
                     NGEDAPIVIGRSGETLRAVEFLTNVALRGEDPGRRVRIDSGGFKARRQESVEKIALAA
                     AREARRKGRPIRLEPMSSWERRIVHVSLKDREDVSTCSTGSEPLRRVVVTPVQKGSQG
                     TA"
     gene            complement(11284..12075)
                     /locus_tag="PLM30_01325"
     CDS             complement(11284..12075)
                     /locus_tag="PLM30_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOR53841.1"
                     /translation="MSALWKAAGDFLLFVLNSLYSFSHSYGIAIILLTVLIRILLHPL
                     SQKQLVSMQKMQKLQPRIKILQEKLANDKDTLNKEIMKLYKENNVNPAAGCLPLLVQL
                     PILILLFRVLMNLDLGGATFLGISLEGSVLSTLGEAVGSTAATHGVGQTFSAIAANPA
                     GLAHVGVYAANLLLLLGVGFLTWFQQQLSGSGNPQMQFMNWFMPAFLTFICLSLPGGV
                     LLYWGVSSLIGVGQQWHIKKKTEVEMRDKPVLLKEKPQKKEGGRP"
     gene            complement(12072..12365)
                     /gene="yidD"
                     /locus_tag="PLM30_01330"
     CDS             complement(12072..12365)
                     /gene="yidD"
                     /locus_tag="PLM30_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOR53842.1"
                     /translation="MTKLPAWVAIKAIIFYQRFISPLLGDRCRFYPSCSSYTIQAIEK
                     FGLARGTALAFLRIMKCGPWHSGGYDPVPDRFPSFFGRVLHRREYSPKGRWIL"
     gene            complement(12362..12766)
                     /gene="rnpA"
                     /locus_tag="PLM30_01335"
     CDS             complement(12362..12766)
                     /gene="rnpA"
                     /locus_tag="PLM30_01335"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOR53843.1"
                     /translation="MRAFFIFPSQDRLKKGWQFDQVFRTGRRESGALVRLLFMVSPDG
                     QTRYGVAVGKQQGTSPLRSRGKRLLRESLRRLRPWVKEGFWIVCLIKSNALGSGAKEV
                     YQDMAKLFKRAGLLKSEWPGPDWFVDRGQGRR"
BASE COUNT         2468 a         3953 c         3657 g         2612 t
ORIGIN      
        1 cgccgggctc aaggacggtg aaatagacgc ccatgttggc ctgggtgccc gagtgaggct
       61 ggacattggc gtgttcggct ccgaagagct cgcaggccct tttcgccgcg agttcctcgg
      121 ccgattcgac gaactcgcaa ccgccgtagt actttttatg ggggtaaccc tcggcgtact
      181 tgttggtcag caccgaaccc tgcgcctgga gtacgtaagg agggacgaaa ttctcggaag
      241 cgatcagttc gaggctttgc ttttgtctca ggaattcgga ctggacaagg gaatctatct
      301 ccgggtccgc gtccatcata accgacttca tgatatccaa aaccgcccat cccctttcgt
      361 aagctaggtt gacctttata tccgtttggc caatattata cccccggtaa gatccttggt
      421 caggcgatgc ttccccgtcc caggaccggg tcgaactgcc tctgcaggag agcctcgaaa
      481 gccgaaaggt ccatggtgcc caggtccccc tcgccacggt ccctgacgga cagggtcaca
      541 ttctccacct ccctggagcc gatgaccacc atgtaaggta tcttctccac ctgggcgtca
      601 cggatcttct tgcccagctt ctcgtccctg gcgtccgtct ccaccctgta cccgaggccc
      661 ttgagtctcg actccacctc ggaggcgtag ggtgagtgtt cctccttcac cggcacgatc
      721 cggacctgta tcggggagag ccagaagggg aaggccccgg cgtagtgctc gatgagtatc
      781 cccatgaatc tttcgagact ccccaggatc gtcctgtgga gcatgacggg gcgatgctcg
      841 ttcccgtcgg gaccgatgta ggtcatatcg aatttctcgg gcatctggaa gtcgagctgg
      901 atagtgccgc actgccatgt cctcccgatg cagtcctcaa ggtggaagtc gatctttggg
      961 ccgtagaatg ccccgtcgcc cgggttaaga cggaagggca cgccgaactc ctccagggct
     1021 tcctgcaggg ccctttcggc cttctcccac atgagggggt ctcccatggc cttctcaggc
     1081 ctggtggaca actccacgcg gtaggaaaaa ccgaacacgt ccccgtagat gaacctgacc
     1141 aggtccatga tgccgatgat ctcctgtttt atctgccccg gcgtcacgta aaggtgggca
     1201 tcgtcctggg tgaaggacct gaccctcata agcccgtgaa ggacgcccga acgctcatgc
     1261 cgatggacgg tgcccagttc ggcgaccctc atgggaaaat cgcggtaact ccttatcctg
     1321 ctcttgtaga ccagtatccc ccccgggcag ttcatcggct tcacggcgaa gggccggccg
     1381 tcgatctcgg tgaagtacat gttatcgcgg tagtggtccc agtggcccga cctcacccac
     1441 aactcctggt ctaggatgag gggggtcctt atctcggagt agcccctccg ggcatgctcc
     1501 gcccgccaga actccacgag ccggtttatg accaccgtgc ccttggggtg gaagaagggg
     1561 aaccccgggc cctcctcctg gatgctgaaa aggtccagtt ctttccccag tttccggtga
     1621 tccctgcgtt tggcctcttc gatcctgttt atgtgatcct ggagcgcttc ggcggtgtca
     1681 aaggcggtgc cgtacaccct ggtcagcatg atgttcttct cgtcgccccg ccagtaggcc
     1741 cccgccacgg agagaagttt gaagtgcccc agtctcgagg tggagaccac gtggggcccc
     1801 cggcagaggt cggcaaactc tccttgccgg tagatcgaga tgaactcatc gggtatctcc
     1861 gagaccagtt ccagtttgta gacggcacct ctttccctga aaagggccag ggcttgctct
     1921 ctcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggcg gtgagatcga
     2041 ccggttcccc atcgctcctg gcggcgacgg cactagcatc tagactccat cgcgacaata
     2101 cttccccggc cgacagggcc tggggggaaa aaagctctaa tccttctggt cccgcgtatt
     2161 taggcaatgt ccaagcctcc atcctaggta gtggccaacc gcgcccgttg aggcaaaagg
     2221 gccggcgggt tgcattttac agcagcaagt cttcggggtc atccctgcat gaaatgtata
     2281 taatatacag ccctcccggg tttatgcaac atcccgagtt gctccacctc agggtccgat
     2341 cgaatgaggt agcgtcccct ttcgagggct ccgaataggt accccggcgc agggatgtgc
     2401 tgctttggaa agaccggaca caaggaggga tcatgatgag ttggcagttc aacaggatca
     2461 tcataatggc cctggtcttc ggcggcacgg gctggtgcgt ctacaggaac ataaaagccg
     2521 gggagaagaa gtagcgcgcc gatcggcagg tcaaaggcca ggggccgccc cgaaggagcg
     2581 gcccctgctt ttttccgcgg gatcactttc ccgtgcttcc gaagcctccg ctcccccgcc
     2641 ccgtctcgct caggaggtcc ttttccgtcc attcgatcct gctcaccggg gcgaggacca
     2701 tctgggctat cctgtcaccc cgttcaacct cgaaggggtt ttcgcccagg ttgacgagga
     2761 tgaccctcac ctcgccccgg taatcggcat cgatcgtccc cggcgtgttc aggacggtca
     2821 cgccatgttt aagtgccagc ccgcttcggg gtcggacctg gcattcgtac ccctcgggga
     2881 tctcgaggcg gataccggtc cccacggaaa gcctcccgcc gggggggatc atagcgtgct
     2941 cgttcgacct gaggtcgacc cccgatgaaa gcgccgtagc gtaaacgggc agggtcattc
     3001 cctcatcgtc atggaagatg ccgactctca ccgtacccgg catgatcacc tttccctgcc
     3061 cctgtccccg aagtccttcc tccggggccc cccgtggggc ctcctatcac cggagggcct
     3121 gtcgccgcca ggcctccccc cgggcctcgg agttcctccc ctcgggcccc tgtcacggtc
     3181 gctcgagggc ggtagggcct ttatcctctc ctccctggca tcctcgagga ccaaggcgtc
     3241 ggcgaacttc tcagccagtt cgggttcatt tcttctttcc agggcccgcc tcctggagag
     3301 gttgatcctc cccatatcgt cgatctcctt gaccatcacg aggaccgagt ccccggggct
     3361 taaaacatcg tcgaccttgc ccacgtgggt ggtgcttatc tcgctcacgt gaaggagtcc
     3421 ctctttaccc gggaggacct ccacgaaggc gccgaaggcc atgagcctgg tgaccttgcc
     3481 cgagaacacc tccccagcca tgacctccct gacgagatcg tttatcatgt cgagggcctt
     3541 ctcggcagcc tcggtggtgg gcgatgcaac gaaaaccctg ccgtcgtcct cgacatcgac
     3601 cttgacgccc gtctgggcga cgatgttgcg tatggtcttg ccgcccgggc cgatgatgtc
     3661 cctgatcttg tccagcggga ccgtgagggt gtagatcctg ggggcatagg cggaaatatt
     3721 cttgcgtggt tctgcgatga ccgcctccat cttgtcgagg atgaaaagcc tccccctgcg
     3781 cgcctggtcg agagcctttt cgaggatggc tctcgtaatg cccccggctt tgttatccat
     3841 ctggagagcg gttatccctt ccctggtgcc ggtgaccttg aaatccatgt ccccgtaatg
     3901 gtcctccagc ccctggatgt cggtgaggac caccaccttg tcgccttcct ttatcaaccc
     3961 catggcgaca ccggcgacgg cctttttcac agggaccccc gcgtccatca gtgaaaggct
     4021 ggcaccgcaa accgaggcca tggagctgga cccgttggat tccagtatgt ccgagaccac
     4081 cctgacggcg tagggaaatt cctcttctcc cgggagaaga ccccgtacag ccctctcggc
     4141 cagggcgcca tgtccgatct ccctccgtcc cggccccctc atgggccgga cctcccctac
     4201 cgagaagggc gggaaattat agtgaagcat gaaacgcttg gagggctcgt ccagtttgag
     4261 gccgtcgatc agctggtcat cctcccccac cattccaagg gtcgttgtca ccagtgcctg
     4321 ggtctctccc cgggtgaaaa gggccgaacc gtggaccctg gggaggaacc ccacttcgca
     4381 gctgatgggc cggacctcgt ccatggctct tccatcggcc ctcctgccct catcgacgat
     4441 gagggacctc atggccttct tcaccatgcc ttccacggaa gccttgatgt agggttccgc
     4501 ttcggaatac ttttccagca ggctttcgag ggccttcccc gtggcgatat cgatcgcctc
     4561 acccctgggc ttcttttcat ggacctggac ggcctggaag atctcctcgt aaagttccct
     4621 ttctacccac tcgtctatct cgggcaagga cgccgcctcg gggagcttga cgggctcctt
     4681 cccagccttt gcgaccatct cgatctgggc ctggaccagg ttggctatct ccttctgggc
     4741 catctcgagg gcgtcgacca tcacgtcctc ggggatctcc cccgcaccgg cctccaccat
     4801 ggtgatcccg ccggagtggc cgctcacgac caggtccagg gtgctctcct tcatcaggtc
     4861 ctcggtgggg ttgatgacga gccggccgtc gatcctgccg accctgacag ctccgacggg
     4921 acccttccag ggtatgcccg acagcatgag cgcggcggag gcgccgttga tggccaggat
     4981 gttcggcggg gtggactggt ccaccgacag ggtggtcgcc actacgtgga tgtcgttcct
     5041 ggtgtgatca tgaaagaggg accggatgga ccggtcgatg atcctcgagg cgaggatggc
     5101 cgtctccgag ggccttcctt cccttttgat gaaccccccc gggatcttac ccgcggagta
     5161 gaaacgctcc tcgtaatcca ccaggagggg gagaaaatcg agcccctctc ttggcttgtc
     5221 tgaaatacac gcggtcacca ggagggatgt ctctccctgg gtcactctga ccgcgccgtt
     5281 ggcctgcttt gccaacttgc cggtctcgaa ggtcatggtg cgacccccga cttcgacgct
     5341 gaatgtttcc atcattattt tgtttcctcc tcttattttc ttcccttttt ttccgtttaa
     5401 gaatatcaca agcggacaaa aaaggacgag ggcggatgac gccgccctcg tccgggtaac
     5461 tgcacgacct gtacgtcgac ggtaacgatc agtgcctcag gcccagtttc tggatgagat
     5521 cgctgtaacg cccgaaatcc ttgttcctga ggtactggag gagccttctc ctgcgcccta
     5581 ccatctttag gagccccctc tgggagtgat ggtccttctt gtggaccttc aggtgctccg
     5641 tcaaatccct tatccgggtg gtgaggatcg cgatctggac ctcgggcgaa cccgtgtccg
     5701 cgttgtgggt cttgaactcc tcgatgatac cctgtttctt ctccttctca agcacttgcc
     5761 ttcacccctg gcacatctat tcccggaacc aagtagagcg gtcattcgca taacctagta
     5821 acgggtgtcg aaattatatc accatcaagg atacggggca aatcgggcgg accgtgtggc
     5881 aatgaaccaa aactgaacca caaaatagtg atatcataag gctctgctca ttctgcactc
     5941 gacattttct taattatgtg ctttcattct taatacgggt tgcaaagaat tcgatcgaaa
     6001 gggtggagaa acaagatgaa tattctgatc gttgaggaca acagggacct cgtacaggta
     6061 ctgacggaag ggttcaacga actggagttt tcagtggaca gcgcctttga cgggatagag
     6121 ggcttgaaca agatccgcaa aaacgactac gactgcgttg tcctggacat catgctcccg
     6181 gagatggacg gctacgaatt gatcgggagg atgagggaag aggggaagga tactcctgtg
     6241 atcatgctca ccgccaagga ttccatcgag gacagggtgg aaggcctgaa ccggggagcc
     6301 gacgactacc tggtaaaacc cttcgatttt cgcgaactgg tggccagggt caacgccgtg
     6361 acccgcagga gaacggagcc gaagaggacc gtcctccact gcgggcccct gatgctggac
     6421 cccatcgcca gggaatgcaa ggccgaaggg gaaatggtac ccctcaggag gagagagttc
     6481 gatatcctcg aactgctcat gcgctttgag aaccaggtct tctcccggga aaagatcata
     6541 ggtctcgtat ggaagaagga gtacgacggt acgagcaacg tggtcgacgt tcatgtaaaa
     6601 tacctcaggg acaagctccg cccctacaac ctcgacaccg tggttgtgac cgtcagggga
     6661 gtcggctaca aggtgacctg cccggattac gagtaagggg gcttgtaccc cgagaacgag
     6721 agatggggat gctgcagtag atgccaagga ccttggacgg gcggcggccg ttaaggcccc
     6781 gctcgtttcg tttcaggctg atcttctttt ccgccatgac catcatggtg accatcgccc
     6841 ttttcgggat ggccgtgttc acttttttta cctccttcga ggtgagcagg ctcgacgccg
     6901 tgctggcatc gagcatggaa gagacggtct ccttttacga aaagtccggc cggttccccc
     6961 ttcaggtcaa ggggagcagg actctgggcg tagtactcca ggtctacaac caggacaagt
     7021 ctctgatgtt ccatctcccc cccggccttt cggccccctt catacccgag gagctgctgg
     7081 aaaaggcttc cttcggtgta aggcccctca ccttcgtccc ttcgaagaac agtttcctgc
     7141 cgaggttttg gtgggtcctg ccctggaact gcctctcgga tcgcgacctc tggagagctc
     7201 tggtcgccac ggcgcccttc agggacgaag aggtggtcct ggtctccatg gctcccatgg
     7261 cgtggctcct ggaatccagg caattgctcc tctggatgac gatcctggct ggggcggcgg
     7321 gcctgatcgc ctctatcgcg gccgggagcc tacttgccgc gggggccatg agacccatca
     7381 aggccatcaa caaagctctc gcaggggttt ccatcgacaa catgtccatc gacccctctc
     7441 ccttcggctc tgaccgtgag atcggcgaga tcgtgaccca tatcaacagg atgctcaaga
     7501 acctggaaaa aagcatgaag aacctgcagc agttcacctc cgacgccagc cacgaactga
     7561 ggacccccct ggcggtaatg aggggggtcg tggacatcgc ccttctcaag cagagggacc
     7621 ccgaatacta cgtccaaaag ctccaggaac tcacctacca tatcgacgac atgcagaacc
     7681 tcgtgggggc ccttctcgaa ctggccagac ttgacgactt caggggactc gactcgaggg
     7741 aaccggtgga cctcctcctg gtcgcggagg atgccgtggg gaacatggag agcatcaccc
     7801 agaggagggg ccaggtcgtc gaaaaggact tgaaaccggc accgaccctc ggtcggtatg
     7861 tcatggtcct gaggctcgcc aacaacctcc tggagaacgc cagcaagtat tctcccccgg
     7921 gcagcaggat aaagatctcc acctacagtg acatcgggaa aaaccaggcg atcctggagg
     7981 tccgggacag gggctccgga ctcgaccccg aggagatcag ccgatgcttc gaccggttct
     8041 ggagggccga cagttccagg accacgccgg actacggcct cggactgccc ctggtcaaga
     8101 gattcgccct gatccataac gcctccatcg aggtggagtc ccaaaaaggg aaaggatccc
     8161 ttttcagggt ccgcttcccg ctggacaagg aaaccctgga ggggtacgac ctcgaatgac
     8221 aaaaaaagcg ggggggcctt aggccccccc gcaacccatt aaaaacccct agagattagc
     8281 tgacacgtag aaggtgcggc cgtccctgaa gaccagcatg gcgatattct tgaggtccct
     8341 ggaaaggata tcctcaagtt gggtcacgtc cttcacggtc tccccgttga cctcgaggac
     8401 cacgtcacct tccctgaggc ccatcctttg accgaccgag cccgagagga caccggtgat
     8461 gacggccccg cccgatccct ccatgccgta cttctgcctc aattccggcg tcaccggcga
     8521 gaccgatata cccagtttcg aagaactgac tggctcactt ctggcgggtg tctcgtttcc
     8581 gtcggagggc accgtcgaga gggttaccat cctcttgccc tcgacgccct tgctgaggac
     8641 cgtcaactcg accttttcac cggcgaggtg ctggcggatc gccacgacca ggtcgccggg
     8701 gtccttgacc tttcggccgt cgatatcgag gatgacatcg cccctcgcga ccccggcctt
     8761 ctcagcgggg gaccccttga ccacgtcagc cacgagcgcc ccctcaccgg tctttatccc
     8821 gtaggcgccg gcgaactcct tggtaagggg ctgcaggtac accccgagcc aacccctgtt
     8881 gacctttccg tattggatga ggtcatccac gatctgcttg gccatgttca ccgggacggc
     8941 gaaacctata ccctgggcgt aggggacgat ggccgtgttg atgccgatga ccctgccgga
     9001 gaggtccagg agcggcccgc cgctgttacc ggggttgatg gcggcatcgg tctggatgaa
     9061 accatcgaag ttgaggttcc tggcccggac ggaccggttc ttcgccgaca ccacgccgac
     9121 ggtgacgctg ctctcgaagc cgtaggggtt gccgatggca atgacccact cccccacgtc
     9181 aaccttgtcg gaatcaccca tggggaccgt ggggagcccc tcggcgtcga tcttgatcac
     9241 ggcgaggtcg taagaggggt ccttccccac tatcttcgcc gggaaggacc ttccatcgga
     9301 gagggtcacc tcgatggtca tattgtcagg gccgtccacg acatggttgt tggtgaggat
     9361 atagccgtcg tgggtcacga tgaacccgga gcccttgccc ctcatgggca cgtcctgggt
     9421 gaagcgctca aagcgatcgc cgaaaaactc cctgaagaaa gggtcgtcag ggaagggcga
     9481 cgatgaacgc tgaacaatgg cctcggtgtc tatgttgacc accgccggcg ccgtctcttt
     9541 aacgatctta gcgatggggt tgccggtgaa gacatcctgc gccacggccg ggccgggggg
     9601 gagagaaaag acaacggccg ccgaaaggag aagcaggagc gtcgccatga ggattttgga
     9661 tcctttgtat ctattcattc gagccacctc ctgaatgctt tgcgccgatt ttcctccttc
     9721 aggggcggcc tggaatcgtt aattctacgg agatcgggtc accgggtttg aacccaagca
     9781 tgtcggaggc cctgcccctg ttcacagcga tggagacgaa accccagctg tcctcaagag
     9841 cgaccatctc gccggaaccc acgtcggaga aggtccgccc cacagggatg ctgaaaagcc
     9901 aaccgctgct gatcttgatc tctgaatcac ccagcttccc caggagatcc caggggatat
     9961 cggtcctgac ggtcccaaac gcatctacga gggtgacatg gccggagatt ttgcccggtg
    10021 aagcgtgggg cgcgggcagg gggaaacggt tgagttctcc cagggggatc tcttccccgc
    10081 attcctcggg aggtttcccg cccaccaggt cggccaccac gggtgccatg atgtccctcg
    10141 cgtcaaaggt cgggaagacg tgctccatct ctccctgggc acgctcgatg gaccagcacc
    10201 ggctgatgcc ccccgtccgt tccgccgccg ggataaggag cccgttatcg ggcccgacga
    10261 agaggtcacc cctgccggaa cgcaggagca gtatcctcct ctgggtcccc acacccgggt
    10321 ccacgatggc caggtggatg ccggagggca tgtagaggac cgcccctgcg agcatccatc
    10381 cgccggaaat gatgtcaaag ggggggactt cgtggctgat gtctatcacc ggcaaggtga
    10441 cccctctcct caaaaggagt cccttgcaac aagccacggt ctcatcggcc aggccgaagt
    10501 cggaaagaaa ggtgacgcac ctgaattccc cggtcatttc cgaacacccc cgataaatat
    10561 accaccaaca attacgttaa agacccaatg gcttaaaggt cggccagatc aggccgtccc
    10621 ctggctgcct ttttgaacgg gtgtgaccac cactctcctc aagggttcgg aacctgtcga
    10681 gcaggtagag acgtcctccc ggtccttgag ggagacatgg acgatcctcc tctcccaact
    10741 gctcatcggc tcgaggcgga tcggccgccc cttcctccgg gcttccctgg cggcagcgag
    10801 ggcgatcttc tcgacgctct cctgccggcg ggccttgaaa ccgccgctgt cgatcctgac
    10861 ccttcgcccc gggtcctcgc cccggagggc gacgttggtg aggaactcga cggccctgag
    10921 ggtttcaccg gacctgccga tcacgatcgg ggcgtcctcg ccgttcagat cgatgctgtc
    10981 aggccccgtc acctccggct cgacggagag gtccatcctc tcgaggaggc cctccaggaa
    11041 ggggaacgtc ccggcgggaa gcccgtcggg gccttgcgat ctcaaggata gggcgacgtg
    11101 catcttcttg cccaggatcc cgaaaagcct tttgccctcg tccaggacat gaacctctat
    11161 ctcggagggg tccacggacc agcggatggc ccctatgtga cgggcctcat cgagggagtt
    11221 cacatctact tcgaaagtct catgatcttc cacggtatta acccctttca tggcatcagg
    11281 gaactagggt ctgccgcctt ccttcttctg gggcttttcc ttgagaagga ccggcttatc
    11341 gcgcatctcc acctcggtct ttttcttgat gtgccactgc tgccccacac cgatcagcga
    11401 agagaccccc cagtagagga gcacaccccc ggggaggctg aggcagataa aggtgaggaa
    11461 ggccggcatg aaccagttca tgaactgcat ctgcggattg ccgctcccgc tgagttgctg
    11521 ctggaaccac gtgaggaagc ccacgcccag caggaggaga aggttggcgg cgtaaactcc
    11581 cacgtgggcc aacccggcgg ggttggcggc tatcgccgaa aaggtctgcc ccaccccgtg
    11641 ggtcgcggcc gtggaaccca cggcctctcc cagcgtggaa agaacggaac cctcgaggct
    11701 gatccccagg aaggtggctc cgcccaggtc caggttcatc aggacccgaa agagcaggat
    11761 cagtatggga agctgcacca ggagtggaag gcagcccgcg gcagggttga cattgttctc
    11821 cttgtagagc ttcatgatct ccttgttcag agtgtccttg tcattggcca acttttcctg
    11881 gaggatcttg atccggggct gcagtttctg cattttctgc atgctcacaa gctgcttctg
    11941 cgagaggggg tgcagaagta tcctgatgag gacggtcaac aatatgatgg cgatgccgta
    12001 ggagtgggag aatgagtaaa ggctgttcag cacgaacagc aggaaatcgc ccgccgcttt
    12061 ccagagtgcg ctcaaaggat ccaccttccc ttgggagaat attctcttcg atggagaacc
    12121 ctgccaaaaa acgacgggaa tctgtccgga acgggatcgt agccgccgct gtgccaggga
    12181 ccgcatttca ttatacgtaa aaaagccaag gctgtgcccc ttgccaggcc gaacttctcg
    12241 atcgcctgga tggtgtagct cgagcaggag ggatagaacc tgcatcggtc gccgagcagg
    12301 ggcgagatga atctctggta gaagataatg gccttgatcg cgacccacgc gggcagtttg
    12361 gtcatcttcg cccttgcccc ctgtcaacga accagtccgg gccgggccat tcgctcttca
    12421 gcaggccggc cctcttgaac agcttcgcca tgtcctggta aacctctttc gcaccgctcc
    12481 ccagggcgtt gctttttatc aggcagacta tccagaaacc ttccttcacc cagggccgga
    12541 gtcgcctcag ggattccctc aaaagtctct tcccccggga gcgaaggggc gaggtcccct
    12601 gctgcttgcc cacggccaca ccatagcggg tctggccatc cggtgagacc ataaacagca
    12661 accgcaccaa cgcgccactc tcccggcggc cggtgcggaa aacctgatcg aactgccaac
    12721 ccttttttag cctgtcctgg gaagggaaaa tgaaaaaagc ccgcaactag accgccaggc
    12781 gctttctgcc
//
LOCUS       DAOSRG010000016        12735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4952_length_12735_cov_9.342823, whole genome shotgun sequence.
ACCESSION   DAOSRG010000016 DAOSRG010000000
VERSION     DAOSRG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4952_length_12735_cov_9.342823"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="rsmH"
                     /locus_tag="PLM30_01340"
     CDS             <1..834
                     /gene="rsmH"
                     /locus_tag="PLM30_01340"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR53844.1"
                     /translation="LGGYSEGVLLKWRKAVVLGIDQDPQAIEAAKRKLMDFGDRFRAV
                     HGNFRDLSPILEKTGMGKPDAVLFDLGVSSIQIETPERGFSFQEDGPLDMRMDSQSER
                     QERAGDIINTAGMRDLITLFRKYGEDPYAAQIARGIVRYRESSGPIKSTSELVQVIRN
                     TLPAPVQRKMGRNPARKVFQALRIAVNDELEALADALNQCESLASGAMMVIVVSYHSL
                     EDRIVKRKFREWDALGRGNDLYRKPLVPGAEELKENWRSRSAKLRAFSFLEKVPEVTD
                     R"
     gene            933..1259
                     /locus_tag="PLM30_01345"
     CDS             933..1259
                     /locus_tag="PLM30_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53845.1"
                     /translation="MGLVGLRLYSLNLEYQLADIQRQIVLMEEEQSRLEKDLAGLLNP
                     TRVYSYARSELGMKASAGIGVVRVAAVRTAPPASQAYDRAEAGVIRKALVWLVDSVSR
                     KASARQ"
     gene            1295..2938
                     /locus_tag="PLM30_01350"
     CDS             1295..2938
                     /locus_tag="PLM30_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR53846.1"
                     /translation="MKGLRKSPWLLLAVFILVLLFRLYTRHLDPDPRIRSQAGSQYWA
                     RLKVAAPRGFIRDRNGIALAISTPALSFFIDPTLWDPGNAPLLQDLIPRERLDRISTK
                     MEGRYFPIARKVDPPVSERIINLKLPGLFWVKETKRIYPQGNTLCHVLGFCSIDDTGL
                     AGTELFWDRVLFAPPEGRIIAKDAKGHFVDVALPGHTLTADNDSLVQLTIDSRLQHIL
                     EQRLKEGMDSHKAEWAAAILVEPSTGMILGMASLPDFNPNVRTTFADKESLRNNALSR
                     VFEPGSTFKPIIMSNLLDNGEVSLDSTINCQGKIRVADVTIHDIKAHGKVNPISIIAD
                     SCNVGMVTGVKHSEPFRTYSFLKRCGFGDPSGIELPGEESGLIMPPSQWRGSVPATIA
                     IGQGIGVTPLQLAMATASIANGGTLMKPFIVSEIRRKGGKLEYQGKPFAVGEVMSART
                     SQLMREAMRQAVEKGTGRTAAVPGVKVAGKTGTAQVPGAGGYLKGDYTPSFVGFWPCD
                     DPKYLLLVVIGNPREGDFLGGKVAAPVFRSIVQDMELIS"
     gene            2960..4504
                     /locus_tag="PLM30_01355"
     CDS             2960..4504
                     /locus_tag="PLM30_01355"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOR53847.1"
                     /translation="MRVSDIKRLLKERGILLGPGVRNIGEEPTGVDAEEDPRITGVFM
                     DSRKVVPGGIFFCLKGTTYDGHDHALEAWTNGAVCVVSERVTPAPVTHLLVRDPRVAM
                     GFAAMAFFDHPADSLKMIAVTGTNGKTTTAYMLRSIFNSTGRRSGMLGTVIYDTGSAA
                     YHEARRTTPESPEIQSMLREMLLNDCNYCVMEASSHGLYQGRLTGCSYDGAIFTNLSP
                     EHLDFHGTMEEYFRSKMILFREHMKTPNWVGASNISDPYGKRVKELFPGRIVSFSLDE
                     EDLADFFGVVKSQSLTGLEMEIFYGGKEPLFQVSLPLTGRFNALNALGAAALSLSMGL
                     DPQTVKKGLESMPQVPGRLQRYSFVNGVTALIDYAHTPEALQNVLTSLREVSEGKIWT
                     VFGSGGDRFKDNRPRMGRVAASLSDQVVITMDNPRSEDPAEIAKEILGGAITGAGRKV
                     PPEVILDRREAIFSVLDRARGGDVILIAGKGPERNIIFSDRVIPHQDSESVEEWSLAR
                     GVKWAK"
     gene            4501..>4688
                     /locus_tag="PLM30_01360"
     CDS             4501..>4688
                     /locus_tag="PLM30_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOR53848.1"
                     /translation="MRGEERPSTGWTLHEAAKAIGLRFVGKNIPMPSHIFVDSRDVLP
                     GSLFVALKGEKADGQDFI"
     assembly_gap    4689..4788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4789..5751
                     /gene="murF"
                     /locus_tag="PLM30_01365"
     CDS             <4789..5751
                     /gene="murF"
                     /locus_tag="PLM30_01365"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOR53849.1"
                     /translation="RRSFNTDIGCAVTVLEAPLGTEILLLELGTNRPGEILETAKTYR
                     PCFGVITEVGPGHLQGLKSIKGVLEAKLELLGAPSIKNLSYNFDNPLLSRALEDSGED
                     LGLFPVGFKSPMYRILEAASVTRDGYPGLDVDIDTPTGERKYRTGLFGEHHAYGLAFA
                     SVLGDRLGVPTTEQVKRLSSFKALPGRGNFYTSAKGYLIIDETYNANPASMTAVLRAL
                     SAIPSPMKKCAVLGGMGELGEDSLQWHRSLIPSFEGLDLVLLVGEPWRSVFQDRPPVN
                     CHTVAFQDLEEIFKNRLSPGDLVLFKGSRCFQMERAIEILENLR"
     gene            5751..6719
                     /locus_tag="PLM30_01370"
     CDS             5751..6719
                     /locus_tag="PLM30_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOR53850.1"
                     /translation="MSSAGLFLAVFLIGTALQGYWIGFLKKRKVIQNLKLYGPQNHLR
                     DKKNIPTMGGAVFLFLALLSGLTLVFWGRWSCQKALLFLGFPLTGGSIGLADDLIKFF
                     RGSSEGLRSLQKLALQVISAVAWSFFLMPGGVLTLAPGRVLEGIPGALVLLFFLVGTL
                     NAANITDGLDGLLSGVMVISFTALLLFIREGDPGRFAALLGLGLSAGFLIFNFHPARV
                     FMGDCGSHFLGGLLVSICAAKGMLLLILPLGFIMGVEVISVIIQIIAIRGFGRRVFSM
                     SPVHHHFEMEGWGERTVVLSFWFFHLVGMGLLLILYKYLPLWTGGY"
     gene            6721..8115
                     /gene="murD"
                     /locus_tag="PLM30_01375"
     CDS             6721..8115
                     /gene="murD"
                     /locus_tag="PLM30_01375"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOR53851.1"
                     /translation="MGAPSEFVKSPRDTRERITVIGAGVSGRALAVFARELGHTVFVS
                     EAKVIDEETRELFHRVGVEYEGGGHSDRMLDGDTMVLSSGVSPGSEPVARAKEKGVRV
                     IGEIDFLAPHLKGSLIGVTGSNGKTTTTLLTGHLLKEAGFRVGVAGNVGSPLASHAGL
                     DHEVLVIELSSFQLFWAHGLRLDIAIVTNLEPDHIDWHGSLGEYYRAKGKIIAMLKSG
                     GTLICQERDLSFLIRDALPAAELYPLSWVKETTEGKGARLLMKGDHATLVSGGTERTL
                     FHYSDVNLLGRHNLENAAMAAASFLLKGGEVDALPASLATFKVPSHRCELVGIVGGVS
                     FIDDSKGTNVAATVAALTSLDGRKVIILGGRGKGEDYGSLANAVQRGAEAVILLGEEA
                     LRIRLALESKGYTKVIEAANMKEAVAQAFKVSPPGGMVLLSPACTSWDMYANYAEKGE
                     DFQRCVIELKREQS"
     gene            8115..9260
                     /locus_tag="PLM30_01380"
     CDS             8115..9260
                     /locus_tag="PLM30_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOR53852.1"
                     /translation="MPRDDSLRGAGETSLGGLNLQATWAIPLVLSCIGILMISSVTAQ
                     LTINQGRTPFFYGLKQLQWLLVGLGGMIACTLPPVAFWRRWSGVIWAGAVLLMILTLL
                     PVLGRSGGGSSRWVRIGPLSLQASEVMLLAAAVHLAKKLNEPGLTRGRAMGRTLLIFA
                     LSAWPFLFQPDLGGVIILFTLSMAVFVQVFGWVIPLVAGTIASVLIFFPVVLLGRYRV
                     ERITSFLDPWNDPLGSGFQTLQGFIAFANGGLFGVGIGHGLQKLQYLPAAHTDFILAA
                     IGEELGMVGTFSVVLLFAILLLIQYHSYRSARDGFEATLTWSVTLAIFIPFFVNAAGV
                     LRLMPLTGVPLPFISYGGSSLVLGWCKIGMLLRLMRQKPYPGEEGIP"
     gene            9581..10336
                     /locus_tag="PLM30_01385"
     CDS             9581..10336
                     /locus_tag="PLM30_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOR53853.1"
                     /translation="MAASRLRGLPLLLHEQNARAGRVTSLAARIGIPVCSGWEICEPL
                     PAGRYLPVGIPVRRLRHLAPREAWERLGYGLDLPPGPRILVLGGSMGSDPLAGLFREL
                     SGEGPFRNYNFFVVGSSNDPVRVADNLLFLPRVWEIDLLYSIADVVISRAGASTLAEL
                     VAFGLPSVIIPWRAAADDHQASNARVFAQKGFGTVWEYGGDTHEKLSFMIDYELKKAR
                     HGQASPFPGMQTSEASEKIWVLLQQIAEGRGRN"
     gene            10333..11778
                     /gene="murC"
                     /locus_tag="PLM30_01390"
     CDS             10333..11778
                     /gene="murC"
                     /locus_tag="PLM30_01390"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOR53854.1"
                     /translation="MNDFDARFDNLERVHLMGIGGAGMSGLALLLRQMGMTITGCDVS
                     HTYYINKISPNGIEFVLGHDKNHLDRFNPDAIVFSSAIPQETEELIEAARRGIRIFKR
                     AEVLSWLFNMRKGIGVAGTHGKTTTASMIGLILENAGLDPTVAIGGELCDIGGNAKLG
                     QGAHMVAELDESDGSFELFRSHVAIVTNADWDHVDYYPNFNSVLEAYERFLGNREHEG
                     TAIVCGEDRGLSALLHNRINGKHQTYGWGARWDWGAQDVRHNRGGGVTYTLSRDGTPT
                     LEISLRISGEHNVLNSLAACAAVNAIGVPLEAASQALKSFKGAKRRLQHMGSVPAIGV
                     DIFDDYGHHPREIAATLGTLRKMFPERRLMTVFQPHRFTRTAAMYREFAEVLSMSDGV
                     FLLPVFPADEMPIEGVSSTLIGDILKQKGHKGFDFCSDMEDVLTKVSANVNEGDVIAT
                     IGAGDVSIVGEKIQQRLEGKGVTIDAMAVKA"
     gene            11756..12685
                     /gene="murB"
                     /locus_tag="PLM30_01395"
     CDS             11756..12685
                     /gene="murB"
                     /locus_tag="PLM30_01395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOR53855.1"
                     /translation="MRWRLRLNRTILPFCSCDIPLKNLTTLGVGGAAECLARPSTRRE
                     VSSVLDFCNEEGISVWFLGGGSNVLIPDEGLRGMTIQTGGLNGVSWEEKGDSVLLEAE
                     AGVPLAALLALSIKRGWRGLEFVAGIPGSVGGSLRGNAGVEGRALGDLVESVLLIDGK
                     GSPRWKNRREIQFSYRYSDLLAEHKAIIACRLSLARAGTAEVALRARDFFNKRVGQPK
                     GVRTAGCIFKNPKSGFAGKILEEAGCKGLKVGAAEVSARHANFIVNNGSANSRDIARL
                     IFICRDRVFEKTGILLDLELCLMGEFLDGEVGH"
BASE COUNT         2703 a         3342 c         3760 g         2830 t
ORIGIN      
        1 ctcggggggt attcggaagg ggttctgttg aaatggagaa aagccgtcgt cctcggcatt
       61 gaccaggatc cccaggccat tgaggcggcg aaaagaaagc tcatggattt tggcgaccgt
      121 tttcgagccg tacacggcaa cttccgcgac ctttccccga tcctggaaaa aaccggaatg
      181 gggaaacccg atgccgtcct cttcgacctg ggggtatcga gcattcagat tgaaaccccc
      241 gagcggggtt tttcatttca ggaggacgga ccgctggata tgcgaatgga tagccagagt
      301 gagcgccagg agagggccgg tgacatcatc aataccgccg gcatgaggga tttgatcacc
      361 ctgttccgca agtacggcga agatccctac gccgcgcaga tagcccgggg gattgtcagg
      421 tacagggagt cctccggccc tatcaagagc acgtcggagc tggtccaggt catcaggaac
      481 acccttccgg cgccggtcca gaggaagatg gggaggaacc cggccaggaa ggttttccag
      541 gcgctcagga tagctgtcaa cgacgaactg gaagcgctgg ccgacgccct gaaccagtgc
      601 gagagcctgg cctcgggggc gatgatggtg atcgtcgtga gttatcactc ccttgaggac
      661 aggatcgtca agagaaaatt tcgggaatgg gatgccctgg gaagaggaaa tgacctttac
      721 cgaaagcccc ttgtgccggg agcggaagaa ttaaaagaaa actggagatc tagaagcgcc
      781 aaactacgag ctttctcatt cctggagaag gtgccggagg tgactgaccg atgaactctt
      841 gcgctctgga attgccccgc tgctgcagtc aaagagtcaa ggcgcgcgga cccttatgga
      901 tccttgtttg cggcggagta gtgtcgctcc tcatgggtct ggtaggtttg aggctttatt
      961 cccttaactt ggagtaccag ctggctgata tccaaaggca gatcgtcctc atggaagagg
     1021 agcagtcgag gttggagaag gacctggcgg gtttactgaa tccaacaaga gtgtatagtt
     1081 atgcgaggtc ggaattgggt atgaaagctt ccgccggcat aggcgtggtg agggtcgcgg
     1141 cagtccggac ggctcctcca gcgagccagg cgtacgatag ggccgaagcg ggcgtcatca
     1201 ggaaggccct cgtgtggctg gtggattccg tgtcgaggaa ggcttccgcc cgacagtgac
     1261 cgtaaattga tcaacgaagg acggtcagta ttggatgaaa ggcctgagga agagcccttg
     1321 gctgttgctt gccgtgttca ttttggtgct cctcttccga ctttatacac gtcaccttga
     1381 cccggacccc aggataagat cacaggcagg aagccaatac tgggccaggt tgaaggtagc
     1441 ggcccccaga ggcttcatca gggacaggaa cggtatagct ttggcgatct ccaccccggc
     1501 cctgagtttt ttcattgacc ccacattgtg ggacccgggc aacgcccccc ttttgcagga
     1561 tttgatcccc cgtgaaaggc ttgacaggat ctccacaaag atggaggggc ggtacttccc
     1621 gatcgctcgg aaggttgacc cacccgtctc cgaacggata attaacctga aacttccagg
     1681 gcttttctgg gtaaaggaga cgaaaaggat ctatccccag gggaacacac tgtgccacgt
     1741 cctgggtttt tgctctatcg atgacacggg actggccggg acggaattat tctgggaccg
     1801 tgttcttttc gcgccgcctg aaggaaggat catagccaag gatgcgaagg gccatttcgt
     1861 ggacgtagcc ctgccaggcc ataccctcac cgcggataat gactcactgg tccaacttac
     1921 catcgactcc aggcttcagc acatcctcga acagaggctc aaggagggga tggacagtca
     1981 caaggctgaa tgggcggcgg cgattctggt cgaaccttcg acgggaatga tcctgggtat
     2041 ggccagcctg cccgatttca acccaaatgt caggaccacc tttgccgata aagaatccct
     2101 taggaataac gctctctcaa gggttttcga gcctggctca acattcaaac ccattattat
     2161 gtcaaacctc cttgataacg gtgaggtctc tctggatagc accataaact gccaagggaa
     2221 gatcagggtg gcagatgtta caatccatga cataaaggcc cacggcaagg tgaacccgat
     2281 cagcataatc gccgattcct gcaacgtagg gatggtcacc ggcgtgaaac actctgaacc
     2341 cttcaggacc tactcttttt taaaaaggtg tgggttcggg gacccctccg ggatcgaact
     2401 ccccggggaa gaatcgggtt tgatcatgcc cccgtcccag tggcggggtt cagtgccggc
     2461 cacgatagcg atcggtcagg gtatcggcgt cacacccctt caattggcca tggccacggc
     2521 ttccatcgcg aacggtggaa cactgatgaa gccctttatc gtttccgaga tcaggcgcaa
     2581 aggcggcaaa ttagaatacc aaggcaagcc tttcgccgtt ggcgaggtta tgtcggctcg
     2641 gacctcgcaa ttgatgcggg aagccatgag gcaggccgtg gaaaagggga cgggaaggac
     2701 tgctgctgtt cctggggtca aagtggcagg caaaacgggt acggcccagg ttccaggagc
     2761 gggagggtat ctcaagggcg attacacccc ttcctttgtg ggcttctggc cctgcgatga
     2821 cccaaaatac ctgctcttgg tcgtcatcgg caacccgagg gagggagact tcctcggcgg
     2881 caaagtggct gctcctgtct tcaggagcat cgtacaggac atggaattga tctcctgaac
     2941 agggggtttt tatccagtga tgagggtctc cgatatcaag aggctactga aggagcgcgg
     3001 aatacttctc gggcccgggg tcaggaacat cggggaggaa ccaacgggag tcgacgctga
     3061 agaggacccc aggataaccg gtgtcttcat ggactctcga aaagtggtgc ccggcggcat
     3121 tttcttttgc ctcaagggga ctacttatga cggccatgac cacgccctcg aggcctggac
     3181 caatggagcg gtgtgtgtcg tctcggagag agtcacacca gcccctgtca ctcatctcct
     3241 ggtccgcgat cccagggtcg cgatgggatt cgctgcgatg gccttttttg accaccctgc
     3301 ggactcgctg aagatgatcg ctgtaaccgg aaccaatggc aagacgacca cggcctacat
     3361 gttgaggtcc atttttaatt ctaccggccg ccgatcgggg atgctgggca ccgtgatcta
     3421 cgacacggga agtgccgcct atcatgaagc ccggaggacg acacccgaat cgcctgaaat
     3481 acaatccatg cttcgtgaaa tgttgctgaa cgactgtaat tactgcgtaa tggaggcctc
     3541 ttcccacggc ctttatcagg ggaggctgac cggctgcagt tacgatgggg ccatttttac
     3601 aaacctttca ccggagcacc tggacttcca tgggaccatg gaggaatatt tcaggtctaa
     3661 gatgatcctt ttcagagaac acatgaaaac cccgaactgg gtaggcgctt cgaatatttc
     3721 tgacccctac ggtaaaaggg tcaaggaact cttccccggc aggatagtat cattctctct
     3781 ggacgaggaa gatctcgccg attttttcgg cgttgttaaa agccaaagcc ttacgggtct
     3841 ggaaatggag atcttttacg gaggtaaaga accgcttttt caggtcagcc tcccattgac
     3901 ggggcgtttc aatgccttga acgcccttgg ggcggccgcc ctctcccttt ccatgggtct
     3961 cgatccccaa actgtaaaaa aggggctcga gtccatgccc caagtgcctg gacgcctgca
     4021 gcgatactct ttcgttaacg gagtgacggc cttaatagac tacgcccaca cccctgaagc
     4081 gcttcagaac gtcctgacct cactgagaga ggtcagcgaa ggaaagatct ggaccgtctt
     4141 cggctcaggg ggtgaccgtt ttaaggataa ccgtccacgg atggggaggg tcgctgcttc
     4201 gctgtcggac caggttgtca ttacgatgga taaccctaga agtgaggacc ctgccgaaat
     4261 tgccaaggag atcctcggcg gggccattac aggggccgga agaaaggtac ctcccgaagt
     4321 catcctggat aggcgcgagg cgatcttttc cgtccttgac agggcccgtg gaggcgacgt
     4381 gatcctcatc gccgggaaag ggcctgagag gaacataatt ttttcggaca gggtcatccc
     4441 tcaccaggat tcggagtcgg tggaggagtg gtccttagca aggggggtaa agtgggcgaa
     4501 atgaggggcg aggaaaggcc ttcaacgggt tggaccctcc acgaagcggc gaaggcgatc
     4561 ggcctccggt tcgttggaaa gaatatcccc atgccctccc atattttcgt tgacagcagg
     4621 gatgtacttc caggtagcct gttcgtcgcc ttgaaggggg aaaaggccga cggccaggat
     4681 ttcatcgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag aaggagcttt
     4801 aacaccgata taggctgtgc cgtgaccgta ctcgaggctc ccctcgggac agagattctt
     4861 ttgcttgaac taggaacgaa tcgccccggc gagatcctcg aaacggcgaa aacctatcgc
     4921 ccgtgtttcg gtgtcataac cgaagtggga ccgggccacc ttcaaggtct gaaaagtatc
     4981 aagggggtcc tcgaggccaa gttggaactc ctgggagccc cttcgataaa gaacttgtct
     5041 tataatttcg ataacccgct gttgtcccgt gccctcgaag actcgggtga ggatctgggc
     5101 cttttcccgg taggtttcaa aagcccgatg taccgcatcc tcgaggctgc gagcgtcacc
     5161 agggacggct atccaggcct agacgtcgac atcgataccc ctacgggtga gcggaagtac
     5221 aggacgggtc ttttcggtga acatcacgcc tatggcctgg ccttcgcctc ggtcctcggt
     5281 gatcgtctgg gggtgccaac gaccgaacag gtgaagaggc tttcctcctt caaggccttg
     5341 ccgggcaggg gaaacttcta cacctcggct aagggctacc tgataatcga tgagacctac
     5401 aacgccaacc cggcgtcgat gaccgccgtt ctcagggctc tatcggcgat cccctcgcca
     5461 atgaaaaaat gtgccgtcct tggaggaatg ggggaactgg gggaagattc cctgcaatgg
     5521 cacaggtcct tgatcccctc ctttgagggg ctggacctcg tcctgctggt gggtgaacct
     5581 tggaggtcgg ttttccagga taggccgcct gtcaactgcc atactgtcgc ttttcaagac
     5641 cttgaagaga ttttcaaaaa ccggctttcc ccgggcgacc ttgtactttt taagggctcc
     5701 cgttgcttcc aaatggaaag agccattgaa atactggaga accttcgata atgtccagtg
     5761 ccgggctctt tctcgcggtt tttctgatcg ggaccgccct gcagggatac tggatcggtt
     5821 ttctgaagaa acggaaggtt atacagaacc tcaaactcta tggcccccag aatcacctca
     5881 gggacaaaaa aaacatcccc accatgggag gcgcggtttt cctttttttg gcgctccttt
     5941 ccgggttgac ccttgttttt tggggtagat ggtcctgtca gaaggctctt ttgttcctgg
     6001 gttttcccct caccggcggt tccatcgggt tggccgatga ccttatcaag ttcttcagag
     6061 gatcgagcga ggggttacga agcctccaga aacttgcttt gcaggtcatc tcggccgtcg
     6121 cctggtcctt tttcttaatg ccaggcgggg tgctgaccct ggccccgggc cgagtgctgg
     6181 aggggatacc aggagcgctg gtcctcctgt tcttcctggt gggaaccctg aacgccgcaa
     6241 acatcaccga cgggcttgat ggactgctat cgggcgtcat ggtgatctcc tttacggcac
     6301 ttcttctctt catccgcgaa ggagatccag gcaggttcgc tgcgttgctg ggattgggcc
     6361 tttcggcggg ttttttgatc tttaactttc acccggccag ggtattcatg ggggattgtg
     6421 gttcccactt cctcgggggg cttcttgtct cgatatgcgc tgccaagggc atgctgctgc
     6481 tcattcttcc cctgggattc attatggggg tcgaagtgat ttccgtcatc attcaaataa
     6541 tagccatcag gggattcggg agaagggtct tttcgatgag ccctgtccat catcattttg
     6601 agatggaggg gtggggcgaa aggacggtcg ttttaagctt ctggtttttc catctcgttg
     6661 gtatggggct gcttttgatc ctttacaagt acctccctct ttggacagga ggatactgac
     6721 atgggcgcgc cttcggagtt cgtgaagagc cctagggata ccagggaaag gatcacggtg
     6781 atcggtgcgg gcgttagcgg cagggccctt gcggttttcg ccagagaact ggggcacacc
     6841 gttttcgtct ctgaagccaa ggtcatcgac gaagaaaccc gcgaactgtt tcaccgcgtc
     6901 ggtgtggaat atgaaggagg cggccattcc gacaggatgc tggacggtga caccatggtg
     6961 ctcagttccg gggtgtcccc cggctccgag ccggtcgcca gggcaaagga gaagggtgtt
     7021 agggtcatcg gagagatcga tttcctggcc cctcatctta agggatccct cataggcgtg
     7081 acgggaagca acggtaagac cacaaccact ctcctcacgg gccacttgtt aaaagaagcg
     7141 ggtttccggg tgggtgtcgc cgggaacgtc ggcagccccc tagcgagcca cgcgggtctg
     7201 gatcatgaag tcctggtgat cgaactcagc agcttccagc tcttttgggc ccatggcctt
     7261 cgtcttgata tcgccattgt caccaacctg gaaccggacc acatcgactg gcacggttca
     7321 ctgggcgagt attacagggc aaagggaaag attatcgcga tgctgaaaag cggcggcacc
     7381 ctgatctgcc aggaaagaga cctttcgttc ctgatccgtg acgccctgcc agcggccgag
     7441 ctctatccct tgagctgggt gaaggagacg accgaaggga aaggggcaag gctgttaatg
     7501 aaaggcgacc atgccaccct ggtctccggc ggcaccgagc ggactctttt ccactatagc
     7561 gatgtaaacc tcctcggcag gcataacctc gagaacgctg ccatggccgc ggcctccttt
     7621 ctcttgaaag ggggcgaggt ggatgccctg cctgcctctt tggcgacttt caaggtgccc
     7681 tcccaccgct gtgaacttgt cggaatcgtg ggcggtgttt cattcattga tgactccaag
     7741 ggcaccaatg tagccgccac cgtggccgcc ctgacctctc tcgacggacg aaaggtcatc
     7801 atccttggcg gcaggggcaa gggggaggat tacggatcgc tggccaatgc cgtccaaagg
     7861 ggggcggagg cagtgatcct gctcggcgag gaagcccttc gcatcaggct ggccctggag
     7921 tcaaagggct atacgaaggt catcgaggcc gcgaacatga aagaggcagt ggcgcaagct
     7981 ttcaaagtat ccccgccggg gggtatggtc ctgctttctc cggcgtgcac cagttgggat
     8041 atgtacgcga actacgccga aaaaggggaa gactttcaaa ggtgcgtaat agaactgaaa
     8101 cgggagcagt cctgatgccc agggacgact cgcttcgggg cgctggcgaa acctcgctcg
     8161 ggggcttaaa tctgcaggcc acatgggcta tcccccttgt cctttcctgc atcgggatcc
     8221 tgatgatatc ctccgttaca gcccaactga cgatcaacca gggaaggaca ccgttcttct
     8281 atggcctgaa acagctgcag tggttacttg tgggccttgg ggggatgatc gcatgtaccc
     8341 tcccacccgt ggccttctgg agaagatgga gtggggtgat atgggctggg gcggttcttt
     8401 tgatgatctt gaccctttta ccggtattgg gccgatcggg cggaggttct agccgatggg
     8461 tgaggatagg ccctttgagc cttcaggctt cagaagtgat gctcctcgct gcagccgtcc
     8521 acctggcgaa gaaactgaat gagccaggtc ttaccagggg aagagcgatg ggccggaccc
     8581 tgctgatctt cgccctttcg gcctggccct tcctgttcca gcccgatctt gggggggtaa
     8641 taatcctttt caccctttcc atggctgtat tcgtccaggt cttcggatgg gttatacccc
     8701 ttgtcgccgg taccatcgca tccgtgctga tcttctttcc ggtggtcctt ttggggcgtt
     8761 accgcgtcga aaggatcacc agttttctgg atccctggaa cgaccccctc gggagcgggt
     8821 ttcagaccct ccaggggttc atcgccttcg ccaacggggg cctgttcggg gtgggcatag
     8881 gacacggact gcagaaactc caatatctgc cggcggcgca caccgatttc attctggccg
     8941 ccatcggcga ggaattggga atggtcggga ccttttcggt agttcttctt ttcgccattc
     9001 tgttgctgat ccagtatcac tcctatcgtt ccgcgaggga tgggtttgag gcgaccctga
     9061 cctggtctgt caccctggcg atatttattc cctttttcgt gaatgccgcg ggggtcctta
     9121 gattgatgcc cctgacggga gtgcctcttc ccttcataag ctacgggggc agctcactgg
     9181 ttttgggatg gtgcaagatc ggaatgctgt tgcgcctgat gaggcagaaa ccctaccctg
     9241 gagaagaggg gatcccatga agatccttat cgcggcgggt gggacagggg gacatatctg
     9301 gcctgctcta tcctttcgcg actggattca aagaaaagga atgtccgcgg agaccctttt
     9361 tttatccgga agccgtgaac ttgaagaaga actgtacgct tccgcgggtg ttgcccccgt
     9421 ggtccttccc attgccgggt cacccttatg gggtccgctg ggaaccagga tctcgcgatc
     9481 cattggtctt gcgcgatcac tgaaggtcgt cctgcaggtc atcagggatt tttcacccga
     9541 tatttgcctt ttgttcgggg gctatgtctc cgtcgccgcc atggcagcct cgagattgcg
     9601 agggttgccc cttcttctgc acgagcagaa tgccagggca ggcagggtga cctctttagc
     9661 ggccaggatc ggcattcccg tctgttccgg ttgggagata tgcgaacctc ttcctgccgg
     9721 tcgttatctt cctgtcggca tcccggtaag gcggttgaga cacctggccc cccgtgaagc
     9781 ctgggaacgg ctaggctacg gattggacct tcccccagga cccaggatcc ttgtccttgg
     9841 gggctccatg gggagcgacc cactggccgg gctgttcagg gagttgtccg gagaagggcc
     9901 tttccggaat tacaatttct tcgtggttgg ttccagcaat gatcccgtca gggtcgccga
     9961 taaccttctg ttcctgccac gggtatggga gatagacctt ctttattcca tcgcggatgt
    10021 tgttatttca agggctggag cttccacact ggcggaactg gttgcatttg gcctgccttc
    10081 tgtcataatt ccctggagag ccgcggcgga cgaccaccag gcatccaacg cgagggtttt
    10141 cgcacaaaaa ggttttggga ctgtttggga gtacggcggc gatactcatg aaaaactttc
    10201 gttcatgata gactatgaac tgaagaaggc taggcatggt caagcctctc cgtttcccgg
    10261 gatgcaaacc agtgaagcca gtgaaaagat ctgggtactt ttacaacaaa tcgccgaagg
    10321 gagaggtcgc aattgaacga cttcgatgcc cgattcgata accttgaaag ggtgcacctt
    10381 atgggcatag ggggagccgg aatgagcggc ctggctcttc ttttgcgcca gatgggaatg
    10441 acgatcacag gatgtgatgt atcacatacc tattacataa ataagatctc ccccaacggc
    10501 atcgaattcg tactgggcca tgacaagaac caccttgacc gtttcaaccc cgacgcgatc
    10561 gttttcagca gcgccatccc ccaagagact gaagaattga tcgaggctgc tagaagaggc
    10621 atccggatat tcaaaagagc ggaagtgctc agctggctct tcaatatgag aaagggtatc
    10681 ggcgtggccg gcacccatgg taaaaccacc acagcctcaa tgatcggcct gatcctcgag
    10741 aatgctgggc tcgaccccac cgtcgccata ggcggggagc tctgcgacat cggcgggaac
    10801 gcgaagctgg ggcaaggagc ccacatggtc gccgaacttg atgaaagtga tgggtccttc
    10861 gagctctttc gttcccacgt ggccatagtg accaatgccg actgggacca tgtcgattat
    10921 taccccaatt tcaactccgt gctcgaagcc tacgaaaggt tccttggcaa cagggaacac
    10981 gaaggcacgg ccatagtctg cggagaggac agggggctct ccgccctcct tcacaacagg
    11041 atcaacggga agcaccagac ctacggctgg ggcgcccggt gggactgggg cgcccaagac
    11101 gtaaggcaca acaggggggg aggggttacc tacacccttt ccagggacgg cacaccgacc
    11161 ctcgaaatat ccctccggat ctccggcgaa cataacgtcc ttaattcgct cgctgcctgc
    11221 gctgcggtga acgcgatcgg agttcccctt gaggcggcct cccaggcact gaagtccttc
    11281 aagggggcga aaaggcgact gcagcacatg ggatcagtcc ccgccatcgg tgtggatatt
    11341 tttgacgact atggccatca ccccagggag atcgctgcca ccttgggcac actcaggaaa
    11401 atgtttcccg aaagaagact catgaccgtt ttccagcccc atcgcttcac gagaacggca
    11461 gctatgtaca gggaattcgc cgaggtactc tccatgtccg acggggtatt tctcctgcct
    11521 gtctttcccg ccgacgaaat gcctattgaa ggcgtctctt cgacgctgat cggagatatc
    11581 ctgaagcaaa aagggcataa gggttttgac ttttgcagtg atatggaaga cgtcttgacc
    11641 aaagtctcag ctaatgtgaa tgagggcgat gtgatcgcta ctatcggcgc cggggatgtt
    11701 tccatcgttg gggaaaagat ccagcaacgc cttgagggga aaggcgttac catcgatgcg
    11761 atggcggtta aggcttaatc gtaccatcct tccgttctgt tcctgtgata tccctctgaa
    11821 aaatctaact accttggggg tgggcggggc cgcggaatgc ctggccaggc cttcaactcg
    11881 ccgcgaagta tcctcggtcc tcgatttctg caatgaggag gggatatcag tctggttcct
    11941 cggtggaggc agcaatgtct tgatccctga tgaaggactt aggggcatga ccattcagac
    12001 gggtgggctt aacggcgttt cctgggaaga gaagggggat tccgtcctgc tcgaagctga
    12061 agcgggagtt cccctggctg ctcttctggc tctttcgata aaaagggggt ggcgggggct
    12121 ggaattcgtc gcaggtatcc ctggaagtgt aggaggatca ctccggggga atgcgggggt
    12181 tgaagggcga gctctcggag acctagtcga atcggtgctg ttgatagacg gaaagggctc
    12241 ccctcgatgg aaaaatagaa gggagatcca gttttcctat cgctactccg acctactggc
    12301 ggaacacaag gccatcatcg cttgcaggct ctcccttgcg agggctggga ctgccgaggt
    12361 ggccctcagg gcaagggatt tttttaacaa gagagtggga caacccaagg gcgtcaggac
    12421 ggctgggtgc atcttcaaaa atccaaagtc gggtttcgcg ggaaagatcc tggaagaggc
    12481 cggctgtaag ggtctcaagg taggcgccgc agaggtatcg gcccggcacg caaatttcat
    12541 tgtgaataat ggttcggcca actcaagaga tatagcaagg ctgatcttca tctgcaggga
    12601 tagggttttc gaaaaaacag ggattctcct ggacctagag ctatgcctta tgggtgagtt
    12661 tcttgatgga gaagtcggcc attagggttg ccctgtttct ggttttcctt tcaggggtgc
    12721 tatactgtac ggaag
//
LOCUS       DAOSRG010000017        12654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_4998_length_12654_cov_9.640527, whole genome shotgun sequence.
ACCESSION   DAOSRG010000017 DAOSRG010000000
VERSION     DAOSRG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_12654_cov_9.640527"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="coaBC"
                     /locus_tag="PLM30_01400"
     CDS             complement(<1..668)
                     /gene="coaBC"
                     /locus_tag="PLM30_01400"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOR53856.1"
                     /translation="MTLGWKKDRRLLLGICGGISAYKTPELVREFKKLGWEVETVLTT
                     AAERFVSPLVISTLSGRRAWLEEDFLSREEGWKIPHINLVEWADVTLIAPATASFLHK
                     AAWGDASDILSASILASKKPILIFPAMNVFMWGHRAVRENVHRCRELGYGVFEPEEGF
                     LACGYEGKGRLPSPGVIREEVLRTLEPTKDMKGTRVLITSGPTREAIDPVRFISNHSS
                     GKMGA"
     gene            complement(640..909)
                     /gene="rpoZ"
                     /locus_tag="PLM30_01405"
     CDS             complement(640..909)
                     /gene="rpoZ"
                     /locus_tag="PLM30_01405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOR53857.1"
                     /translation="MTNKAERANDPSILSNKYLLACIIAKRARQLSEKKGRLNLEEGD
                     LYFNPIQQALREIGEGKIILEFPETDPATTPEGDDKDDPGVEKGS"
     gene            complement(920..1516)
                     /gene="gmk"
                     /locus_tag="PLM30_01410"
     CDS             complement(920..1516)
                     /gene="gmk"
                     /locus_tag="PLM30_01410"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR53858.1"
                     /translation="MTPIAKGRLFVISGPSGVGKGTLRKALFKSVQGLEYSVSCTTRE
                     PRNREIDGVDYQFIDLDDFKDLVKEGRFLEWAEVHGNFYGTLKENVKRILESGRDAIL
                     EIDVQGALQVKESDPEAVLIFVMPPSEEELRSRLKRRGTEKEEDLRNRISGSRDEMLL
                     SARYDHLVVNDDLGRALESLRRIIEGYRDPQRSPKSFS"
     gene            complement(1500..1766)
                     /locus_tag="PLM30_01415"
     CDS             complement(1500..1766)
                     /locus_tag="PLM30_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOR53859.1"
                     /translation="MGYNLLHIGFGNMLVVERVVAIVHPSSAPIKRLKDEARSQGKLI
                     DATEGRKTRAILITDSDHVVLSAIQPETIVVRFEGKEKEGDPDR"
     gene            complement(1771..2655)
                     /locus_tag="PLM30_01420"
     CDS             complement(1771..2655)
                     /locus_tag="PLM30_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOR53860.1"
                     /translation="MKSMTGFSRAVEKGDLFSINLEITSLNHRNQEITVRLPRELSHL
                     EPQIQQSMRAASRRGKIQVRIDITWDPAANSSRIEPEILKGYAKELDRIRQELGLKGE
                     LDLGPLLSLSGVLSSRELDTPELEDILGDTVRNALEKGIQEWDSMRAKEGDHLKEAVL
                     DSLETYKETAEAIREKWSLSRDRALEAQKERIRQILETDGACVDENRVAQEIVLAADK
                     WDITEELARSSSHVDKFLGLVHNDEVSGKTLDFLLQEMNREVNTIASKVQDSEVRWLV
                     VEAKGFLERIRELVQNVE"
     gene            complement(2652..3785)
                     /gene="hflX"
                     /locus_tag="PLM30_01425"
     CDS             complement(2652..3785)
                     /gene="hflX"
                     /locus_tag="PLM30_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOR53861.1"
                     /translation="MIELQRSPFQKAFLVGLEMGCKEDGPGTLEEMTLLLENLGITCA
                     GKTIQRRSEPDPGYFVGPGKAELIGTEAKDLQAGLIVVDGILSPGQAGALARVVGLPV
                     WDRPLIIMKIFEDRAQTEEAKLQVELARCRYEIPLLKGLGLQMSRPGGGIGTRGPGET
                     EFERHRRKLARRIREINKKIEAIRDRRESRRKRRTRAGLATIALAGYTNSGKSTLLRR
                     LSGDHDLQVADKLFSTLDPFVRRVRLAGGAAVLFTDTVGFIRNLPPVLVTAFRATLEE
                     ITAADLIVLLADATSGRFEEDLAVVEETLASIGAGTVPRIIAINKADRVDTDTMINLR
                     TLAGRSGDRVVTISALTGENTPELLDVVGEMLFSGRKEERGDR"
     gene            complement(3782..4411)
                     /locus_tag="PLM30_01430"
     CDS             complement(3782..4411)
                     /locus_tag="PLM30_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR53862.1"
                     /translation="MLFYWIVAFFFRIVMKVYNRVTVRGLDRALAVAPPVIIAANHCS
                     NLDPIVIGAFFPRRLRYLAKSELFRFLPLGLAIKALGAVPANREDLQGSAGAVKFLIE
                     RLQAGEDILLFPEGQRSTDGRIQPLEQGVGLLAARTGAPVIPAYIHGTFEAMPRGSRA
                     CRPVKIFLSFGEPLHPASLGSKEVSAKDLRRAVTDLLEASLKELEAVNL"
     gene            complement(4446..5126)
                     /gene="cmk"
                     /locus_tag="PLM30_01435"
     CDS             complement(4446..5126)
                     /gene="cmk"
                     /locus_tag="PLM30_01435"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOR53863.1"
                     /translation="MRIYCHYGSNVITLDGPAGAGKSTIARLLADRLGCEYLDTGALY
                     RAVTLLLLMRSIGTTEEEKISMALKECVVSLEGGKVFIDGRDVTLKIRSPEVDRAVSA
                     YSALPAVRNFLLETQRRTAKGRKVVAEGRDMGSVVFPEASFKFFLDARVEVRAARRLE
                     ELKARGEVITFEEVKTQLSRRDQLDSGRELSPLRVPEKALIIDTSEKTVDEVIGEILA
                     VMAGRRSC"
     gene            complement(5145..5876)
                     /locus_tag="PLM30_01440"
     CDS             complement(5145..5876)
                     /locus_tag="PLM30_01440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOR53864.1"
                     /translation="MTEKQRINRFLASCGVCSRRKADLLVREGRVTVNGETCVDPSRV
                     IDPDRDTVSVDGEIVALQEKLYLVLNKPKGCVCSVSDRFTRTILELLPPDYRAKGLYP
                     VGRLDKESEGLLILTNDGALAQRILHPGQHVLKTYQVLLDTAVSVDFIYSLSRGTGIA
                     GRLVTPVEVALMEKTPKGRWVSITLSEGLKREIRVMASTRGFGVERLVRTRIGQLELK
                     RLKPGEFVSLDRETLLDMIIHGGAV"
     gene            complement(5873..6463)
                     /gene="scpB"
                     /locus_tag="PLM30_01445"
     CDS             complement(5873..6463)
                     /gene="scpB"
                     /locus_tag="PLM30_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOR53865.1"
                     /translation="MTSGYRGENPLSPIARAVEAVLFVAVQPVGVKELASVTGSTEEV
                     VAGALEEIKERYRNCHGLRLLNIAGGWSMATSPEQEEVVALFRDVALSQRVRLSKAAL
                     ETLAVVAYSQPTTRSEIEEIRGVRSERVLDTLLGHGLIKVAGRRKGSGTPLLYRTTDS
                     FLSLFGLNSISDLPTLEEMQDILPVDEMSDDGGRDI"
     gene            complement(6441..7175)
                     /locus_tag="PLM30_01450"
     CDS             complement(6441..7175)
                     /locus_tag="PLM30_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOR53866.1"
                     /translation="MRLDVELEGFSGPLDVLCHLVESGGISASGISVSQLIRVYSEFL
                     IENERATINEMAAFFSLIARLLLGKLKALLPPSPGEEQAEFDRGSEAELIESVLERYG
                     PYRAAAAGLAGMMQTRGRSFTRGGSEEGPPWFDLGDLYSLCMVWWEMVRRRREGAGAA
                     PAVLEEEWDGIPTVTPEEEQVEKRMVEIEILLSSKGSLQMSQVFGTVFSRQAFVVTFL
                     AILEMARLGRVHLVQEVLFGDVRLSR"
     gene            complement(7205..8197)
                     /gene="trpS"
                     /locus_tag="PLM30_01455"
     CDS             complement(7205..8197)
                     /gene="trpS"
                     /locus_tag="PLM30_01455"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOR53867.1"
                     /translation="MKKRVFSGMRPTGRLHLGHLAGALSNWIRFQDEYDCFYCIVDWH
                     AMMSDYSDTRFMKDNCREVLLDWLGVGLDPDRSTLFLQSHVPHHAELHLALSMITPLG
                     WLERCPTYKDQILNIQNKDLSTYAFLGYPVLMAGDIMLYKAELVPVGEDQSAHLELTR
                     EVVRRFNHFFGEVFQEPQTILTKTPKIPGTDGRKMSKSYGNAINIADDPEEIWEKLRT
                     MVTDTARERRSDPGNPDKCPVWDLHKAFNHDMEQRAYIDKGCRTAGIGCIECKQALYS
                     HLMAILEPIQSRRRAFEANEEDLLEVLKKGAMKAEKVSEVTIEEVIKAIGFISR"
     gene            complement(8235..9032)
                     /locus_tag="PLM30_01460"
     CDS             complement(8235..9032)
                     /locus_tag="PLM30_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR53868.1"
                     /translation="MSTLLRVRDIIDGIDTFAPFSSCRDWDNSGLQVGSLDWEVSRLA
                     VSLDLTDRAIDESLEKGCSCIVTHHPVIFEPLRNIETGTSLGRKILTCLERHLSVISV
                     HTNWDICTGGVNWTLAGLIGLEPISSLDGSDGATGWMGVHGAFLTPVKGTALPAVLRE
                     KWCLSWSRDYRIPDTVSYLALAGGAGGDLWPVAKSRGSEVFVTADMKYHQVMEAVESS
                     LGVIVADHGEMERITLPALGSLLSETLRLEVLQVDALGFEAGNIATE"
     gene            complement(9080..9156)
                     /locus_tag="PLM30_01465"
     tRNA            complement(9080..9156)
                     /locus_tag="PLM30_01465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9120..9122),aa:Ile,seq:cat)
     gene            complement(9210..9392)
                     /locus_tag="PLM30_01470"
     CDS             complement(9210..9392)
                     /locus_tag="PLM30_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOR53869.1"
                     /translation="MKVKINTEECIGCGVCAQICPEVFELDEDAGKARVIRPEGAECA
                     PEAADSCPVSCITVEE"
     gene            complement(9489..10286)
                     /locus_tag="PLM30_01475"
     CDS             complement(9489..10286)
                     /locus_tag="PLM30_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOR53870.1"
                     /translation="MKSTRTERLIRIASRFLALPSRQISLTEMSEQFKVSKTVISEDM
                     AIIDKAFREEGVGTLKVGRGRSGGAAMKPGFSGNDRSSFLGKVAAVLSSNDRILPGML
                     IHYSDILFNPAFARPLGYALASDFAGKEPDVVMTSEVKGIPLAIFTAHALGVPMAVCR
                     FRNRPGDGPAVAVHYPSGSGEVRTMFMGTRFLTSGKRALIIDDFMRGGSTAAGMLLVA
                     KEFGAEVIGIGIFIAASEPEKKAVSEYRSLLRLERGPDGTPVVKVRR"
     gene            complement(10294..11163)
                     /locus_tag="PLM30_01480"
     CDS             complement(10294..11163)
                     /locus_tag="PLM30_01480"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53871.1"
                     /translation="MTHEINSPGKINLTLRITGIRSDGMHNIASLFMRLPALEKLTLG
                     IRSEDNGGKDLVRVHGQRIKDRNILQAVLEVARKKDPNMPPIIVDIWKQVPPGSGLGA
                     GSGNAAALASWLSRDLGVRFSVEELVGIGSDVPFLFRGGDLSWMTGAGEKPLHRINGV
                     AGLFRVVIAIPGWSCSTPVMYRRADGFYRKEGWKYCEEEACEEALGILGPLKRRRRVG
                     LLPNDFLPVLLAMHPGYRSLFEAAENSGALAWGISGSGSAFFCLFNRENGDASPLGLF
                     EEADFVRKILVLG"
     gene            complement(11230..11481)
                     /locus_tag="PLM30_01485"
     CDS             complement(11230..11481)
                     /locus_tag="PLM30_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOR53872.1"
                     /translation="MSPNLATIRDRVNEFKGSKVRYKALKGRRKMEERMAVIVETYPH
                     LFTLYVESQRSTVSFSYAELLTREVEMEILPGNKKLIPY"
     gene            complement(12017..>12654)
                     /locus_tag="PLM30_01490"
     CDS             complement(12017..>12654)
                     /locus_tag="PLM30_01490"
                     /inference="COORDINATES: protein motif:HMM:NF020671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HOR53873.1"
                     /translation="VDSGEGLIVNGDMARKKLGQVLSFLKEYHQEEPDSPGAPADALF
                     RRILPEMELRHAKKVVERWAREGEIEVEDQRVRLPGFFPGDAARRGAAGAVILDLCST
                     RSFQLPEIADVLEGTGLRQDEVEKALEALRSQGQIFFAGSGFIVSREVLEGLIGILEG
                     FKDDITVASVRDATGSSRKYILPLLEMLDSMGVTRRVQDKRILRKGKLGQD"
BASE COUNT         2636 a         3903 c         3220 g         2895 t
ORIGIN      
        1 gcccccatct ttccggagct gtggttgctg atgaacctga cgggatcgat agcctctctt
       61 gtcgggcctg aagtgatcag aaccctggtc cccttcatgt cttttgtcgg ttcaagcgtc
      121 cttaaaacct cttcccttat gacccctgga gagggtagcc ggcccttccc ctcataaccg
      181 caagcaagga agccttcctc gggttcgaaa accccatacc caagttccct gcagcggtga
      241 acgttctccc ttacggcgcg gtgcccccac atgaaaacgt tcatggccgg gaagattaaa
      301 atcggttttt tagacgccag gatggatgcc gagaggatgt ccgaggcatc accccaagcg
      361 gccttgtgga gaaaggaagc cgttgccggt gcgatcagcg taacgtccgc ccattccacg
      421 aggttgatgt gagggatctt ccaaccctct tcgcgggaga ggaagtcctc ttcaagccaa
      481 gcccgcctgc cggaaagggt cgaaatgacc agtggtgaaa cgaacctttc cgcagcagtt
      541 gtaaggactg tttcgacttc ccaaccgagc ttcttgaact cccggacgag ttcaggagtc
      601 ttgtaggccg agataccgcc gcagatcccg aggagaagtc tacgatcctt tttccacccc
      661 agggtcatcc ttatcgtctc cttcaggagt ggtcgcgggg tcagtttcgg ggaactcgag
      721 gatgatcttg ccctcgccga tctcccttag cgcctgctgg atggggttaa aatacagatc
      781 cccctcctcc aggttcagac gccccttctt ttcgctcagt tgtcttgccc gtttcgcgat
      841 gatacaggct aaaagatact tgttggacaa tatggatggg tcatttgccc tttcggcttt
      901 atttgtcacg ggtatcacct cacgaaaagg atttggggga tcgttggggg tcccggtagc
      961 cctctattat tctcctcaat gattcaaggg cccgaccgag atcgtcattg acgaccaggt
     1021 ggtcgtatct tgccgagagg agcatctcgt ccctggaccc actgatacgg ttcctcaggt
     1081 cttcctcttt ctcggtcccc ctccttttaa gcctgctcct gagctcttcc tccgatgggg
     1141 gcataacaaa gatcaacacc gcctccggat ccgactcctt tacctggagt gccccctgga
     1201 catcgatctc caggatcgcg tcgcggccgc tctcaaggat ccttttcacg ttttccttca
     1261 gggtaccgta aaagttcccg tgtacttcgg cccactcaag gaagcgtccc tctttgacaa
     1321 gatccttgaa gtcatcgagg tcgatgaatt gatagtccac cccgtcgatc tccctgttcc
     1381 ttggctccct ggtagtgcag gagacggagt actccaatcc ctgaactgac ttgaaaagag
     1441 ctttcctgag ggtgcccttc cctacaccgc tcggccccga gatcacgaaa agccgtccct
     1501 tagcgatcgg ggtcaccctc cttttcctta ccctcaaacc tgacgacgat ggtctcaggc
     1561 tgtatcgccg acaggaccac gtgatcgctg tcggtgataa ggatggccct ggtcttcctt
     1621 ccctcggtgg catcgataag cttgccctgg cttcttgcct cgtccttcag acgcttgatg
     1681 ggtgccgacg aggggtggac gatggccacg accctctcga ccactagcat attaccgaaa
     1741 cctatgtgta aaaggttata acccatggcc tcactccacg ttctggacca gttccctgat
     1801 cctttccagg aagcccttgg cttcaacgac caaccatctt acttccgaat cctgcacctt
     1861 cgaggcgatc gtgttgacct cccggttcat ctcctggagc aggaaatcga gggttttccc
     1921 ggagacttca tcattatgga ccaagccgag gaatttatcc acgtggcttg aagacctggc
     1981 gagttcctcc gttatatccc atttgtcggc ggcgaggact atctcctggg ctacacggtt
     2041 ctcatcaaca caggctccgt cagtttcgag aatctgtctg atcctctctt tctgggcctc
     2101 aagagccctg tcacgggaaa ggctccactt ttccctgatc gcctcggccg tctccttgta
     2161 cgtctccagc gaatcgagga ccgcttcctt gagatgatcc ccttccttcg ccctcatgga
     2221 atcccattcc tggataccct tttccagggc gttcctcacc gtatcgccga gaatatcctc
     2281 aagttcgggg gtatccagct ctcgggagga aaggacgccg gagagcgaca gaagtggccc
     2341 caggtccaac tcccccttca gccccagttc ctgccttata cgatccagtt ctttagcgta
     2401 gcctttcagt atctcgggtt ctattctcga ggagttggcg gcagggtccc aggtgatgtc
     2461 aatcctcacc tgtattttgc ccctcctaga ggcggccctc atactctgct ggatctgggg
     2521 ctccagatgg gacaactccc ttgggagcct cacggttatc tcctggttcc tgtgattcaa
     2581 gctcgttatc tccaggttga tcgagaaaag gtctcccttc tccaccgccc tgctgaatcc
     2641 tgtcatgctt ttcatcgatc ccctcgctcc tcctttcggc ccgaaaaaag catctccccc
     2701 acaacatcga ggagttccgg ggtgttctcg cccgtaaggg cgctgatggt aacgaccctg
     2761 tcacccgacc tcccggccag ggtcctgagg tttatcatcg tatcggtgtc aaccctatcc
     2821 gccttgttta tggctattat ccttgggaca gtccctgccc ctatggaggc cagcgtctcc
     2881 tccaccacgg ccaggtcctc ctcaaatctc ccggaggtgg cgtccgctaa gaggacgata
     2941 aggtccgccg ccgtgatctc ctcaagggtg gcacggaagg ctgtcaccag cacggggggc
     3001 aggtttctta tgaaacctac ggtgtcagta aaaaggacgg cggcgccccc agcgagccta
     3061 acccttcgga cgaaggggtc gagggttgaa aaaagcttat cggcgacttg aaggtcatga
     3121 tctcccgaaa gcctcctgag gagcgttgat ttgccgctgt ttgtgtaacc cgcgagggct
     3181 atggtggcga gaccagccct cgtccttcgt ttccgccggc tttccctcct atctcttatg
     3241 gcctcgatct tcttgtttat ctcacggatc cttcttgcca gctttctcct gtgcctctca
     3301 aactccgtct cacccggccc cctggtccct attccacccc cgggtcgcga catctggagc
     3361 cccagccctt taagcagggg tatctcgtat ctgcatcggg ccagttctac ctggagttta
     3421 gcctcttccg tctgggccct gtcctcgaag atcttcatga tgatcaaggg cctgtcccag
     3481 acggggagtc ccaccactcg ggccagggcc cccgcctggc caggagagag gatcccgtcc
     3541 acgacaatga gccccgcttg gagatccttg gcctcggtcc cgatcagttc cgccttgcct
     3601 ggccccacga agtaaccggg atccggctcc gaacggcgct ggatggtttt gcccgcgcag
     3661 gttatcccga gattttccag gagaagggtc atttcttcca gggtgccggg gccgtcttcc
     3721 ttgcagccca tctccagtcc gaccaggaaa gctttttgga aaggtgatcg ctggagttcg
     3781 atcaaagatt aaccgcctcc agttccttga gtgaagcttc aaggaggtcg gttacggccc
     3841 tcctcaggtc ctttgcggaa acctccttcg atcccagcga tgccgggtga aggggctctc
     3901 cgaaggagag gaagatcttt acgggcctgc aagcccggga accacgaggc atcgcctcaa
     3961 aggtcccatg gatataggcg gggatcacgg gggcaccggt ccttgccgca aggagtccca
     4021 ctccctgttc caggggctgg atccttccgt ccgtactcct ctgaccttca ggaaagagca
     4081 gaatatcttc accggcctgg agcctttcga tcaggaactt cacggcccct gcggaaccct
     4141 gcaggtcctc cctgttggct gggacggccc ccagggcctt gatggcaaga cccaggggca
     4201 ggaaccggaa tagttctgat ttcgccaagt accttagcct cctcgggaaa aaagcgccta
     4261 tcacgatggg atccaggttg ctgcagtggt ttgccgcaat aatgaccggg ggggcaacgg
     4321 ccagcgccct atcgagaccc ctcaccgtta ccctgttata gaccttcatc actatcctga
     4381 aaaaaaaggc tacgatccag taaaaaagca ctctcaagtt gggacctccc ggggtcatac
     4441 tatgttcaac agctcctcct gcctgccatt acagccagga tctcacctat gacttcgtcg
     4501 accgtttttt ccgaagtatc gatgattagg gctttttcag ggactctcaa gggtgagagt
     4561 tccctcccgc tatccagctg gtcccgcctg gaaagctgag tcttaacctc ttcaaaggtt
     4621 atcacttcac ccctagcctt cagttcttcc aacctcctgg cggccctgac ttcaaccctg
     4681 gcatccagga aaaacttgaa ggacgcctcg ggaaagacca cgctccccat gtctcttccc
     4741 tcggcgacga cctttcttcc cttggcggtc ctcctctggg tctccaacag gaaattcctc
     4801 acagcaggga gcgcggagta ggcggaaacg gcccgatcca cttccgggga acggatcttc
     4861 agcgtgacgt cccggccgtc tatgaacacc ttccctccct caagagagac gacgcattcc
     4921 ttcaaagcca tggagatctt ttcttcctct gtggttccaa tggacctcat taagagaaga
     4981 agggttaccg cccgatacaa ggccccggtg tccaggtatt cgcaaccgag cctatcggcc
     5041 agcaaccttg cgatggtgct cttcccagca ccagcgggac cgtcaagggt gatgacattg
     5101 ctgccataat ggcagtagat tctcatcgga aattcctccc caccctacac ggcaccgccg
     5161 tgaatgatca tgtccaggag cgtctccctg tccagagaga caaattcccc cggttttagc
     5221 ctcttcaatt cgagctgtcc gatcctggtc cggacaagcc tttccacccc gaagcccctg
     5281 gtggatgcca tgacccttat ctctctcttc aagccctccg aaagggtgat agacacccag
     5341 cgccctttgg gcgttttctc catgagtgcg acctccaccg gcgtcaccag ccttcctgcg
     5401 atcccggtcc ccctcgaaag ggaataaata aaatctacgg aaacagcggt gtccagcagg
     5461 acctggtaag tttttaggac gtgctgcccg ggatggagta tcctctgggc caaggcgcca
     5521 tcgttggtca gtatcaagag accttcgctt tccttatcca gcctgccgac cggataaagg
     5581 ccctttgcgc ggtagtctgg gggcagcaat tccaagatcg tccgggtgaa ccggtccgaa
     5641 acggaacaga cgcacccctt cggtttattc aggacaaggt acaatttctc ctggagggcg
     5701 actatctccc catcgacact cacggtatcc ctgtcgggat cgatgactcg ggaaggatcg
     5761 acacaggtct ccccgttcac cgtgaccctc ccctcccgaa ccagcaggtc cgccttcctt
     5821 ctcgaacaga cgccgcagga ggccaaaaat ctgtttatcc gttgcttctc ggtcatatat
     5881 cccttccccc gtcatcggac atttcgtcta cgggaagaat atcttgcatc tcctcgaggg
     5941 ttggtaggtc cgagatcgag ttgagcccga agagtgagag gaacgaatca gtagttcgat
     6001 aaagcaacgg cgttcccgaa cctttcctcc tcccggccac ctttataaga ccatgcccga
     6061 gaagtgtatc gagtacccgt tcggacctga ctcccctgat ctcttcgatc tccgaccgcg
     6121 ttgtaggctg cgagtaggcg acgacggcga gggtttcaag ggccgccttg cttaaccgca
     6181 ccctctggga gagcgccacg tcacgaaaca gcgcgaccac ttcctcctgt tcaggagaag
     6241 tggccatcga ccacccgccg gctatattca aaaggcggag tccatggcaa ttccgatacc
     6301 tttcctttat ctcctcgaga gccccggcca cgacctcctc cgtcgatccc gtgactgaag
     6361 ccagttcctt tacgcccacg ggctggacag cgacgaagag gacggcctct acggcccttg
     6421 ctatcggaga aaggggattt tcaccgcgat aaccggacgt caccaaaaag aacctcctgg
     6481 accaggtgga ccctccccag cctggccatt tcaaggatcg ccaggaaggt caccacgaaa
     6541 gcctgccttg aaaaaacggt cccgaagacc tggctcattt gaaggctccc tttgctcgag
     6601 agcagtattt cgatctccac catccttttc tctacctgct cctcctccgg ggtcaccgtc
     6661 ggaatgccgt cccattcctc ttcgagaacc gcgggcgcgg caccggcgcc ttctcgtctc
     6721 cggcgaacca tctcccacca caccatgcaa agcgaataga ggtcgcccag gtcgaaccag
     6781 gggggacctt cctcagaacc gcccctggtg aaactgcgcc cccgggtttg catcattccc
     6841 gccaggccgg ccgccgccgc cctgtaaggc ccgtaccttt ccaggacgct ttcgatcaac
     6901 tcggcctcgg atcctctatc aaactcggct tgctcttccc caggggaagg aggcagaagg
     6961 gccttgagtt taccgagcaa aagccgggcg ataagggaga aaaaggcggc catctcgttg
     7021 atagtcgccc tttcgttctc gatcaaaaat tcggaataca ccctgatcaa ttggctgacg
     7081 cttatccctg aggcgcttat gccccctgac tcgacaagat ggcagagcac atcgagcggc
     7141 ccggaaaaac cctcaagctc tacatcgagt ctcattcagg cctccatctt ggtgagttcg
     7201 cccttcatcg gctgataaaa ccgatagcct ttattacttc ctctatggtc acttctgata
     7261 ccttctcggc tttcatcgct ccctttttca ggacctcaag gaggtcctcc tcattggcct
     7321 caaaggccct gcgcctggac tggatcggct ccaggatggc catcagatgc gaataaagtg
     7381 cttgtttgca ttcgatgcag ccgataccgg cggtacggca tcccttgtcg atgtaagctc
     7441 tctgttccat atcgtggttg aaggccttgt gcaggtccca gacgggacac ttgtccgggt
     7501 tgcccgggtc actccgcctc tccctggcgg tatcggtgac catagtcctc aacttttccc
     7561 atatctcctc cggatcatcg gcgatattga tagcatttcc ataggacttg ctcatttttc
     7621 ggccgtcagt accagggatc ttgggggtct tcgtgagaat ggtctggggc tcctggaata
     7681 cctccccaaa aaagtggttg aaccgccgga cgacctccct ggtcagttcc aggtgggccg
     7741 actggtcctc acccacggga accagttcgg ccttgtagag cattatatcc cccgccatca
     7801 acactgggta accgaggaaa gcgtaagtgc tgagatcctt gttctggata ttgaggatct
     7861 ggtccttgta ggtggggcac ctttcgagcc agccaagggg cgttatcatg gaaagggcca
     7921 gatggagttc ggcgtgatgg ggcacatggg actgcaggaa tagggtactc cggtcggggt
     7981 caaggcccac accaagccag tcaagaagca cttcacggca attgtctttc atgaagcgcg
     8041 tgtcggagta gtccgacatc atggcatgcc agtccacaat gcaataaaaa caatcatact
     8101 cgtcctgaaa cctgatccag ttggacaggg ccccagcaag gtgcccgaga tggagtctcc
     8161 cggtcggcct catgccgctg aaaacgcgtt ttttcatcaa tgctcacccc gtttgaagat
     8221 ttggcgaaaa acggtcattc cgtcgcgata ttccccgcct cgaaacccag agcatcgacc
     8281 tggaggactt ccaggcggag ggtttcggac aggagggacc ccagggcagg gagggtgatc
     8341 ctctccattt ctccatgatc cgcgacgatc accccaaggc tgctttcgac ggcttccatg
     8401 acctgatggt acttcatatc ggcggtgacg aacacttcag agcccctcga cttcgccacc
     8461 ggccaaagat ccccgcctgc cccacccgca agggcaagat atgacacggt atcgggtatg
     8521 cggtaatccc tggaccagga tagacaccac ttttcccgga gaacagcggg gagggcggtt
     8581 ccctttaccg gggtcaaaaa agcaccgtga acccccatcc acccggtcgc gccgtcgctt
     8641 ccatccagag aagagatcgg ctccaggccg atcaatccgg ccagggtcca gttgactcca
     8701 ccggtgcata tgtcccagtt cgtgtggacc gagataaccg aaagatgcct ttcaaggcat
     8761 gtcaggatct ttcttcccag ggacgtcccg gtctcgatat ttctgagggg ctcaaaaata
     8821 acggggtgat gggtgacgat gcaggaacat cccttttcca gggattcgtc gatggccctg
     8881 tcggtcaagt ccagcgatac cgccaatcgg ctaacctccc aatcgaggct acctacctgc
     8941 aggcccgagt tatcccaatc gcggcatgag gaaaaggggg cgaaggtatc gatcccatca
     9001 atgatatccc tgacccgaag caacgtgctc accgcctaaa aaataaagcc accgcaggtg
     9061 gctttatcat catttagcgt ggtgggccca cctggactcg aaccaggaac cttccggtta
     9121 tgagccggtg gctctgacca gttgagctat gggcccattc ttggaaaaac cgcaagacgg
     9181 gaggatccgt tctaccgccg acaggtctct tattcttcga cggttataca acttacaggg
     9241 cagctgtcag cggcttctgg ggcacactcc gccccttcgg gacggatcac cctggccttt
     9301 ccggcgtcct catcaagctc aaaaacctcg ggacatatct gagcacatac accgcaaccg
     9361 atacactctt cggtattgat cttcaccttc atggagacga gccacctcct cccattgggt
     9421 gattctatcc cacttgagag cccacgtcaa acaacagggt caagagccgt aaggccgaaa
     9481 cacggccatc atcgccgtac cttcactacg ggcgtaccat cgggtcccct ctccagcctg
     9541 agcagggatc ggtattccga aacggctttc ttttccggtt ccgatgcagc gatgaagatc
     9601 cctatcccga tcacctcggc cccgaattcc ttggccacga gaagcatgcc ggcggcggtg
     9661 ctaccgcctc tcatgaagtc atcgatgatg agggccctct tccccgaagt aaggaacctc
     9721 gtccccatga acatggtcct cacctcaccg ctgccggagg ggtagtgtac cgccacggcc
     9781 ggaccatctc ccggcctgtt ccggaagcgg cacactgcca tgggcacccc cagggcatgg
     9841 gcggtaaaga tcgccagggg tatccccttg acctcggagg tcatgaccac atcaggttcc
     9901 ttgccggcga aatccgaagc cagggcgtaa cccaggggcc gggcgaaagc ggggttaaaa
     9961 aggatgtccg agtaatggat gagcatgccg ggtaggatcc tgtcattgga tgaaagcaca
    10021 gcggcaacct tcccgagaaa ggatgagcgg tcatttcctg aaaagcctgg tttcattgct
    10081 gcaccaccgc ttcggcctct tcctaccttc aaggttccga caccctcttc gcggaaggct
    10141 ttatcaatga tggccatgtc ttcgctgatg accgtcttgg acactttgaa ctgttccgac
    10201 atctcggtca gggagatctg cctcgagggc aaggccaaaa aacgcgatgc gatcctgatc
    10261 aacctctcgg tcctggtact tttcataagg ccatcacccc aagacaagga ttttccgcac
    10321 gaagtcagct tcttcaaaca aacccaaggg agaggcatcg ccattttcgc ggttgaaaag
    10381 gcaaaaaaag gcgctcccgc tcccgcttat accccaggcc agcgcgccgc tgttttccgc
    10441 ggcttcgaag agagatcggt accccggatg cattgctaaa agcaccggaa ggaagtcatt
    10501 ggggagaaga ccgaccctcc gtcgcctttt cagaggcccg aggataccca gggcctcttc
    10561 acaggcttct tcctcgcagt atttccaccc ttctttccgg taaaacccgt cggctctcct
    10621 gtacataacc ggggtagaac aggaccaccc aggaatggca ataacaaccc taaaaagacc
    10681 ggcgaccccg ttgatcctat ggaggggctt ttccccggca ccggtcatcc aggaaaggtc
    10741 cccgcctcga aaaaggaagg gaacgtcgga accgatccca accaattctt cgaccgaaaa
    10801 ccttacgccc agatccctag aaagccatga agccaacgcg gcggcgttac cgcttcccgc
    10861 accgagcccg ctccctgggg gcacctgttt ccaaatgtct acaatgatgg gcggcatatt
    10921 cgggtccttc ttcctggcga cttccaggac cgcctggagg atattcctgt ctttgatcct
    10981 ctggccgtgg acccttacga gatctttgcc gccattatct tcactgcgga tccccagtgt
    11041 caacttttcc aaggcaggca gcctcatgaa taaagaggcg atgttgtgca tgccgtcgct
    11101 cctgatgcca gttatcctca atgtcaagtt aatttttccg ggggagttga tctcatgggt
    11161 catgacggac acctcttaaa aagaaacccc ggtgcgtcga gcaccgggga aaagtgcgag
    11221 ggggcagggt cagtaaggga taagtttctt gtttccggga aggatctcca tttcgacctc
    11281 gcgggtgagc agttcagcgt aactgaagga tactgtgctt ctctgggatt cgacgtacaa
    11341 cgtgaaaagg tgcgggtaag tctcaacgat cacagccatc cgctcttcca tctttcttct
    11401 gcctttgaga gccttgtacc gcaccttgga gcccttgaac tcgttcaccc ggtctctgat
    11461 cgtggctagg ttaggactca tctcaggttc actcctgttg ttggtttcct aagattttac
    11521 cacaaatgtt cacatctttc aatgtagcgg gaagggaaat taaatacgaa tatcatttca
    11581 cccttcaccg catcgttacc ccgttttcac aatcttggcc ggtgctgttt catttcagct
    11641 atcggaggca ccatcgagcc cttagccgaa gtctaaagta ttgaaaaccg gggcaaagca
    11701 tggctaccca acagggtaag ggacgacggg cctttgacag gtagcgcggc ccctgggtaa
    11761 ccggtggggc gataataatc ggttgtccct gtgagcttgg agcttcatca ggagttccga
    11821 ggaagccttt gtcttgtttt ccaggttcca cccaaggtcc cggagaacac aggttcgcca
    11881 gcgcacgggt gataaacggc cattttaacg gatgggccag ctttctcagc atcgtgcctt
    11941 tccgcaattg cggtggtctt cgccttgacg aacctatgtc ttcaggtttg ggagattcct
    12001 ttctcttaag aaagagctaa tcctggccaa gtttaccttt cctgaggata cgtttatcct
    12061 ggactctcct ggtcaccccc atcgaatcga gcatctccag tagagggagg atatacttcc
    12121 gactgctccc ggtagcatcc ctgacggaag cgacggtgat atcgtccttg aaaccttcaa
    12181 ggataccgat gagtccctcc aggacttccc tggatacgat gaacccactg cccgcgaaga
    12241 agatctgccc ctgggatctc agggcttcca gggctttctc cacctcgtcc tgacggagtc
    12301 ctgtaccttc caagacatcg gctatctccg gcaactggaa ggatcgggtc gaacagagat
    12361 ccaaaatgac ggccccagcg gcacctctcc tggccgcgtc tccagggaaa aaaccgggaa
    12421 gcctgaccct ctggtcctca acttcgatct ccccttctcg tgcccatcgt tctaccacct
    12481 ttttcgcgtg gcggagctcc atttctggga ggatccttct gaagagagca tccgccggcg
    12541 ctcccggact gtccggctct tcctggtggt actctttcaa aaacgacagg acctgaccta
    12601 gttttttcct tgccatatcg ccgttgacta tcaacccttc tcccgagtca acca
//
LOCUS       DAOSRG010000018        12640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5003_length_12640_cov_9.332936, whole genome shotgun sequence.
ACCESSION   DAOSRG010000018 DAOSRG010000000
VERSION     DAOSRG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5003_length_12640_cov_9.332936"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLM30_01495"
     CDS             complement(<1..548)
                     /locus_tag="PLM30_01495"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOR53874.1"
                     /translation="MKTRILKLCFFLFLLFFLFCSPGPASADSFRSAERIVSLAPSIT
                     ESLFAIGAGERVVGVTDHDVSPEEVKALPSVGGYFDPSLEKILSLSPDLVIGIATFHG
                     KLLERLQSLGVDTLPLTIHRGLDGVRAGITSLGSRLGLEKEAGEVLGNIERGLEDVRE
                     EVTQTFPEGSPSLLVVVWHDPLT"
     gene            complement(529..1161)
                     /locus_tag="PLM30_01500"
     CDS             complement(529..1161)
                     /locus_tag="PLM30_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR53875.1"
                     /translation="MELMDLGGPVMWIILVLSVLGGTIVLERFWFIFRASTDAPSLEL
                     ALGELIYHGKKEEASSLVRSRDSSLHRLFRVAVDHWGVGHEAMQVLMEQEVRREVFRW
                     ERGLGFLATLARVAPLLGLLGTVIGMIDVFRNLPGLMGSNMAALAGGIWEALLTTVAG
                     LSVAVPLILFHAFFSARLERAEEMLWRGVDFMVREKVLRSGSATNEDQDP"
     gene            complement(1176..3119)
                     /locus_tag="PLM30_01505"
     CDS             complement(1176..3119)
                     /locus_tag="PLM30_01505"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR53876.1"
                     /translation="MRGLKGVFGGFPVKSLEDEDVDLIKEAARESRAWAVTMTSAAGS
                     GHPAGSLSSMEMYLMVYGAADITPENFPGLERDFVIISHGHTSPGAYAVLARYGFISP
                     ESAMAHFRQVGSPFQGHIERVVPGIDWGSGNLGQGLSAGVGFALAIRARGRQDHVYVL
                     MSDGEQVKGQVAEARRIASHHRLAGITALVDYNDIQISGRTRDIMESDIPGLWRVDGW
                     SVIECDGHDPRALYKALIDARAGDRPTVIICHTVMGKGVSFMEGVPDYHGKAASGDLY
                     VEAMEELGASPALLDEALKGRSTGVFDPPSRPTSIAPSIVTGDPRSYGPDDRTDNRSG
                     FGKALEDVGRLNYKSPGCDPLLVFDCDLAGSVKVEGFAKACPEWFIEAGVQEHSTATV
                     AGAASICGVTSLWADFGVFGLGEAYNQQRLNDINHSNLKLALTHVGLDVGEDGETHQC
                     IDYIGLLRNLFGWRLLVPADPNQADRMTRWALAKPGNVCIAMGRSKVPVVTDDEGNPF
                     FAGDYVFRYGAVDLLRKGRDAAIITMGHMAARALRAREILKGQGFDVSVYHAGCPLHM
                     DTTSIVEAARTGRIVTFEDHHASTGLGSIVASLLLKTGSGCPLRTLGVTRYGDSGASS
                     EVLSAMGLDPENLAAAVRNLIVQ"
     gene            complement(3276..4391)
                     /gene="alr"
                     /locus_tag="PLM30_01510"
     CDS             complement(3276..4391)
                     /gene="alr"
                     /locus_tag="PLM30_01510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR53877.1"
                     /translation="MSLRPTRMEVNLENLAFNFRSISGFLDKGCSLMAVVKANGYGLG
                     ARRVAKVFQEAGCQRFAVATPDEAVELRENGISEPLLVMGPSPQDAAEVFAAMDITAA
                     CTDLSFAEAMSRAAVTQGKTARLHLKVDTGMGRIGFLPEDIPETVDRILHFPGIDLEG
                     IFTHFACADERNPEYTRIQFGRFGEALDALRSRDVRFRIRHVCNSAGTVNFPEMHLDA
                     VRPGLILYGMWPSRYCKRSVEIKPVFEVKTSVAAVRELPASSGIGYGLRYMTKGTEQV
                     AILPIGYHDGYTRPLSMKASVLIRGERAPIVGSICMDQTMVDVTHIPGVIPGDEVVLV
                     GSQGQESVTPEEIASLLGTINYEISSLFGPRVPRAYI"
     gene            complement(4484..>5548)
                     /locus_tag="PLM30_01515"
     CDS             complement(4484..>5548)
                     /locus_tag="PLM30_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOR53878.1"
                     /translation="AIFGMTTKMTEAALAVRYREKDEGGNWRGGTMHVLEKATGQKWL
                     AWLFAFFTTFAAFGIGNATQANSTAQALELGFGVPCWISGIVIVVLVALVIVGGVKRI
                     SDVTTYLVPFMAIFYIVGGGAVILLNIGLVPHAINQAVCYAFNDPMAIPGAIAGWSVK
                     QALTKGVARGVFSNEAGLGSAPMVHATAITDHPVRQGAYGLFEVFVDTIIICSITGIS
                     ILATGTLTSRPDLTGAQLSLTAFQSVLGTPGLWILSLGLAMFAFSTILGWYWYGETGA
                     VYIFGLKITPVYKFLWIAVCLVGAIGGGGQFLTNLWDLSDTMNGLMAAPNLIALLWVS
                     NEMRKMVKDFDAKRKSGELK"
     assembly_gap    5549..5648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5745..6902)
                     /gene="grdD"
                     /locus_tag="PLM30_01520"
     CDS             complement(5745..6902)
                     /gene="grdD"
                     /locus_tag="PLM30_01520"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HOR53879.1"
                     /translation="MTGTETRRIIGDALREIVESAKSGGPRTRVGLMASGSELGPGEL
                     ARGAVAAQESRPGLEVVMIGPRIEGFDQLRWIETPDCEEDISRAMESALKEGDISAAV
                     ALHYPFPLGVTTIGRVVTPARGRPMFIASTTGTSATERIEAMVKNAVYGIATAKSLGI
                     TEPTVGLLNIEGAQAAFRALQKIRDNGFPITFGSSVRKDGGAILRGNDILAGAVDVCV
                     TDTLTGNVLIKMFSAFTTGGGYEATGWGYGPSVGRGWPFIVSIISRASGAPVIAGALV
                     FSAAAATGSLPEKVIEYLALAEKAGLSGILSELRPTKSDGSGEVKMPPKEPTEDEIHG
                     IDVLSIEEAVKTLWKREIYAESAMGCTGPVVKVPARVLEEAEEILRSEGFL"
     gene            complement(6908..8443)
                     /gene="grdC"
                     /locus_tag="PLM30_01525"
     CDS             complement(6908..8443)
                     /gene="grdC"
                     /locus_tag="PLM30_01525"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HOR53880.1"
                     /translation="MARVAIRGYSYCLNHTPELGLHYGVTPYIERITHGETEYIKKLC
                     KSTQSFEEACAYAPNQAYIGGMDLDDLKRQPAPMYCNRIDGASRYGKFGEIMPEKEFL
                     GLMDICDVFDIIWLEESFAGEVREVMARHPLMNEKILTRLEKGRASSEIEKETKEHGA
                     LPLYSQGRVAGCCRKGHDLDESLSAHVLLENIASKAGSVLALLHLLKTTGLSPADIDY
                     IIECSEEAAGDMNQRGGGNFAKAIAEIAGCVNASGFDVRSFCAGPVNAVIAGATQVAS
                     RARPNVVVLAGGAVPKLFMNSRDHVRKDIPALEDCLGSFAVLLDADDGTCPIMRLDAL
                     GKHSVGAGASPQAITSALVYEPLQKIGLSFGDVDKFAAELQIPEITVPAGAGDVPSAN
                     FKMIAALAVMKGELGKDEMSSFVESKGIPGFAHTQGHIPSGVPFIGHACEAIKEGKIG
                     RVMIIGKGSLFLARLTNLSDGASFIIEPSSEKAGETRGVTSEEVRGILLEVLEDLSKE
                     LTG"
     gene            complement(8511..9818)
                     /gene="grdB"
                     /locus_tag="PLM30_01530"
     CDS             complement(8511..9818)
                     /gene="grdB"
                     /locus_tag="PLM30_01530"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8772..8774),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOR53881.1"
                     /translation="MKYKVVHYINQFFAGIGGEEMAGHRPESRKGFVGPGQAFNAALA
                     PDAEIVGTVICGDTYFADNLEKAEKEVLDLIASFSPDAVVAGPAFNAGRYGTACGAVC
                     EAVGKTLGLPRVSGMYEENPGVDMYRKSTYIARTADSARGMKDAVPAMARLLLKQLKG
                     EPIGKPDDEGYIERGVRVNFFMDKIGAERAVDMFVKKLKGEPFTTEYPMPTFDRVPPN
                     PPVADMKSATIALVTSGGIVPKGNPDHIEASSASKYGEYSLAGVMKIDASTHETAHGG
                     YDPTYANDDPNRVLPVDVMRDLEKEGAFKKFHETFYATVGNGTSVGNARRFGTEIAQK
                     LKDANVDAVIVTSTUGTCTRCGATIVKEIERAGLPVVHVCTIVPISLTVGANRIVPAV
                     AIPHPLGDPTKTPEEEKKIRRAIVEKALKALQTPVEDQTVFND"
     gene            complement(9853..10329)
                     /gene="grdA"
                     /locus_tag="PLM30_01535"
     CDS             complement(9853..10329)
                     /gene="grdA"
                     /locus_tag="PLM30_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083775795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(10198..10200),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOR53882.1"
                     /translation="MGKLAGKKVLLLGERDGVAAPAMEESLKNCGADIVFSVTECFVU
                     TAAGAMDLQNQQRVKDAAEKFGAENLVVILGSSDAEGAEIYAETVTNGDPTFAGPLAG
                     VPLGLAVYHVFEQEIRDEADPSVWADQIGMMEMVLETESLANAVKGVREQYGKYTL"
     gene            complement(10374..11660)
                     /locus_tag="PLM30_01540"
     CDS             complement(10374..11660)
                     /locus_tag="PLM30_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOR53883.1"
                     /translation="MELKLKKVVVRSLLDGDKTSFDDGVLKVNRREIVNMLMKDKRFS
                     SAMVELALPGESTRILPVKDVVEPRCKLEGPGEVFPGFIGDVETVGEGTTLALVGAAV
                     VTCGKIVGFQEGIIDMSGPGAEYTPFSKTLNVVLVVEPVEGLEKHEYESACRLAGLKT
                     AHYLATAAKGATGGEVETYCLPDLAKAMTFFPELPKVAYLYMLQSQGLLHDTYVYGVD
                     AKRIIPTLIHPNEVMDGAIVSGNCVSACDKNNTYSHQNNPIIHHMYKRHGKDLNFVGC
                     VITNENTTLADKKRSSSYAVKLAKMLGVEGVIISEEGFGNPDTDLIMNCRKAEQSGIK
                     TVLVTDEYAGRDGASQSLADACPEADAVVTAANANEIIVLPRMEKIIGLPDTANVIAG
                     GFQGSLREDGSIEVEIQAITGATSELGFSRIGAYTI"
     gene            complement(11691..12014)
                     /locus_tag="PLM30_01545"
     CDS             complement(11691..12014)
                     /locus_tag="PLM30_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOR53884.1"
                     /translation="MIELNKENFEEEVLKSDLPVLVDLWGPQCGPCLALMPKVEEISR
                     KYEGKVKFCKLNVAENRKLVISLRVMGVPTFLFYKGGKESGPRLSGAEAVADKIEEKV
                     KELSG"
     gene            complement(12084..12476)
                     /locus_tag="PLM30_01550"
     CDS             complement(12084..12476)
                     /locus_tag="PLM30_01550"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOR53885.1"
                     /translation="MFQEIDCLTNNARLWNISLKATFVEGGPLSVLTLARDRVHAGWL
                     LLTHPLYGNIQPSQQHFRSVLVGLPDEKREGDLGSISILERAIGLYRAEGVNSVKPAR
                     GDRHLEDFAALDVYLLRDSMERYGLLKE"
     regulatory      complement(12579..>12640)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         2453 a         3876 c         3530 g         2681 t
ORIGIN      
        1 gtgagaggat cgtgccaaac gacgacaagc agtgacggcg acccctcggg aaaggtctgg
       61 gtgacttctt ccctgacgtc ctcgagaccc cgctctatgt tccccaggac ctctccggcc
      121 tctttttcca atcccaacct ggagccgagg cttgttatgc cggccctgac cccgtccaga
      181 cccctgtgga tcgtcagggg cagcgtatcc acgccaaggg actggagcct ttccagcagt
      241 ttgccatgga aggtggctat gccgatcacc aggtccgggg aaagggagag gatcttttcc
      301 agggatggat cgaagtaacc tcccaccgaa ggcagggctt ttacctcttc cggtgagacg
      361 tcgtggtcgg tgaccccgac caccctctcc cccgctccga tggcgaagag gctctcggtg
      421 atcgagggcg ccagcgaaac tatcctctcc gcggaacgga aggaatctgc cgacgccggg
      481 ccaggcgaac agaacaggaa aaagagcagg aaaagaaaga agcagagctt aaggatcctg
      541 gtcttcattc gtcgcggatc ctgatctcaa taccttctcg cggaccatga agtccactcc
      601 tcgccaaagc atctcctcag ccctttcgag gcgcgcggag aaaaaagcat gaaacagtat
      661 cagcggcacg gccacggaga gtccggccac ggtagtgagg agcgcctccc aaattccgcc
      721 ggctagggct gccatgttgg accccatcaa acccggaagg tttctgaaaa cgtctatcat
      781 gcctataacg gtccccagga gccccaggag gggcgctacc cgggctaggg tggccagaaa
      841 tccaaggccc ctttcccatc ggaatacttc gcgcctgacc tcctgttcca tcaggacctg
      901 catggcttcg tgccccactc cccaatggtc cacggccacc ctgaagagtc gatgaagaga
      961 actgtccctg ctcctgacca ggctcgaggc ttcttccttc tttccatggt atatcaactc
     1021 tcccagggcg agttcaagcg acggcgcatc ggtggaggct ctgaagatga accagaatct
     1081 ctccagaacg atggtccctc ctaatacgga gagcaccagg attatccaca ttacgggtcc
     1141 gcccaggtcc ataagttcca tggccatgcc ctatcctact ggacgattag atttcttacg
     1201 gcggcggcga ggttttcagg atccaggccc atggcggata ggacttctga agaagccccc
     1261 gaatccccgt acctcgtaac gccaagggtc ctaagaggac agccgctacc ggttttaagc
     1321 aacagggagg ccacgatgga tcccaggccc gtggaagcat gatgatcttc gaaggtcact
     1381 atcctcccgg tacgggcggc ttcgacgatc gaggtagtgt ccatgtgcag tgggcaccca
     1441 gcgtgataga ccgatacatc gaaaccctgg cccttcaaga tttcacgcgc cctcaaagct
     1501 ctcgcggcca tgtggcccat ggtgatgatg gccgcgtccc tgccctttct gagcaggtcc
     1561 accgcgccgt atcgaaagac atagtcgccc gcgaaaaagg gattcccctc atcatcggtc
     1621 accaccggta ccttgctccg ccccatcgct atgcagacgt tccccggttt ggcaagggcc
     1681 cagcgggtca tcctgtccgc ctggttggga tcggccggaa cgaggagccg ccacccaaac
     1741 aggtttctca agaggccgat gtagtcgatg cactggtggg tttcgccatc ctctccgaca
     1801 tcaaggccga cgtgggtcag ggcgagtttc aggttggagt ggtttatgtc gttcaacctc
     1861 tgctggttat aggcctcccc caagccgaag acaccgaaat cggcccacag gcttgtaacc
     1921 ccgcagatgg aagccgcgcc ggcgacggta gcggtggagt gctcctggac tccggcttcg
     1981 atgaaccact ccgggcaggc ttttgcgaat ccctccacct tgacggaacc agcgaggtcg
     2041 cagtcgaata caagtagggg gtcacaccca ggggacttgt agttgagcct gcccacatct
     2101 tcaagggcct tgccgaagcc ggacctgttg tcggtccggt cgtcggggcc ataactcctg
     2161 ggatcgccgg tcactattga tggcgctatc gatgtcggcc ttgacggggg gtcaaagacg
     2221 ccggtggacc gccctttgag ggcttcgtcg agaagcgccg gtgacgcgcc gagttcctcc
     2281 atggcctcca cgtagagatc tcccgaggcc gccttgccat gataatcggg gaccccctcc
     2341 ataaaggaga cgcctttgcc catcacggtg tggcagataa tcaccgtcgg ccggtcaccg
     2401 gccctggcat cgatgagagc tttgtaaagg gccctcgggt cgtgcccgtc gcactctatg
     2461 acactccacc cgtcgaccct ccagaggccg ggaatgtccg actccattat gtcccgagtc
     2521 cggccggaga tctggatatc gttgtagtcc accagggccg ttattcccgc caatcggtga
     2581 tgggaggcga tcctcctggc ctcggccacc tgccccttga cctgttctcc atcgctcatc
     2641 agcacataga catgatcctg cctacccctg gcccttatgg caagggcgaa gccgacgccg
     2701 gccgagaggc cttgtccaag gttgccgctc ccccagtcga tccccggaac aaccctttca
     2761 atgtgcccct ggaagggact gccgacctgg cgaaagtgag ccatggcgct ttcgggggat
     2821 atgaaaccgt acctggcaag gacggcatag gctcccgggg aggtatgccc gtggcttata
     2881 attacaaaat ccctttcaag gcccgggaag ttttccgggg taatatcggc cgcgccgtag
     2941 accatcaggt acatctccat gctcgaaaga gaaccggcgg ggtggccgct gccggccgcc
     3001 gaagtcatgg tcacagccca ggcccggctc tctcgcgcgg cttcctttat caggtctaca
     3061 tcctcatctt cgagggattt taccgggaac cctccgaaga cacctttcag gcctctcata
     3121 aggcaaacct ccccgtgata ttcaaaaaat gaacaaagct acccttcgcg actcatccag
     3181 gattatacct ccaacggccg aacataaaaa aacggcgaca cgcggtcgcc gcaaggtttt
     3241 agctcaccag tgccgggaaa ggtcattgcg acccttcaga tgtacgccct cgggactctt
     3301 ggcccgaaca ggctcgaaat ctcatagttg atcgtgccaa gcagtgaggc gatctcttcc
     3361 ggcgtaacgg attcctgccc ctggcttccc acgaggacca cctcgtcgcc gggaatgacc
     3421 ccgggaatgt gggtcacatc gaccatggtc tgatccatgc agatgctgcc tactataggt
     3481 gccctttcgc ccctgatgag gaccgaggcc ttcatcgaga ggggtcttgt gtaaccatcg
     3541 tggtagccga tcggtagaat ggccacctgt tccgtcccct tggtcatgta tcggaggccg
     3601 tagccgatgc cgctggaggc cggcagttcc ctgacggcgg ccaccgaggt tttcacctcg
     3661 aagacgggct tgatctcgac actcctcttg caataccggg aaggccacat tccatacagg
     3721 atgagcccgg gcctcacagc gtcgaggtgc atttcgggga aattcaccgt gccggcgctg
     3781 ttgcaaacat gcctgatcct gaacctgaca tccctggagc gcaaggcgtc cagagcctct
     3841 ccgaaccttc cgaactggat acgggtgtac tcggggttcc tttcatcggc gcaggcgaaa
     3901 tgggtgaaaa tgccttcgag gtcgattccg gggaaatgaa ggattctatc gacggtctca
     3961 gggatgtcct ccgggagaaa gcctatcctt cccataccgg tgtcgacctt caggtgcagg
     4021 cgcgctgtct tgccctgggt taccgcagcc ctgctcattg cctcggcgaa ggacaagtcc
     4081 gtacaggcag cggtaatatc catggctgcg aagacttcgg ccgcatcctg cggcgaagga
     4141 cccataacga gcagcggttc gctgattccg ttttctctta attccacggc ctcatcgggc
     4201 gtggccacgg cgaaacgctg acaccccgcc tcctggaaga ccttggcgac ccgccttgcg
     4261 ccgagaccgt agccgttggc ctttacgacc gccatgaggc tacagccctt atccaggaac
     4321 cccgaaatgg acctgaaatt gaaggcgaga ttctccaggt tgacctccat cctggtaggt
     4381 ctaagggaca agggtatgcc ctcctttcaa aagggaaggg gggcagggaa tccccggccc
     4441 cctttttaag cggaaaacca gtttcagcct tgaaaggaaa atactatttc agctctccgc
     4501 tcttgcgttt tgcgtcgaaa tccttgacca tcttgcgcat ctcgttggaa acccaaagga
     4561 gcgcgataag gttgggggcc gccatgagcc cgttcatggt gtccgacagg tcccaaaggt
     4621 ttgtaaggaa ctgtccgccg ccaccgatgg cgccgaccag gcacaccgcg atccagagga
     4681 acttgtaaac cggggtgatc ttcaggccga agatatagac ggcgccggtt tcaccatacc
     4741 agtaccatcc gagtatggtc gagaaggcga acatggccag accgagggac aaaatccaca
     4801 agcctggcgt ccccaggacc gactggaatg cggtgagaga gagctgggcg ccggtgaggt
     4861 cgggcctcga cgtgagggta ccggtagcaa ggatgctgat gccggtgatg gagcagatga
     4921 ttatggtgtc cacaaacacc tcgaagaggc cgtaagcacc ttgcctcacg ggatgatccg
     4981 tgatggcagt ggcgtggacc atcggggcgg agccaaggcc ggcctcgttc gagaagaccc
     5041 ctcgggcgac gcccttggtg agggcctgct tgaccgacca tccggcgata gccccgggaa
     5101 ttgccatggg atcattgaaa gcgtagcaga cggcctggtt tatggcatgg gggaccaggc
     5161 ctatgttcaa caggatgacc gccccaccgc ccacaatgta gaaaatggcc atgaagggaa
     5221 ccaggtaggt agtcacatcg gagatccttt tgacccctcc gactatgacg agggccacaa
     5281 gcacaacgat gacgatgccg gaaatccagc agggaactcc gaaacccagt tcaagcgcct
     5341 gggcggtgga gttggcctgg gtggcgttac caattccgaa agccgcgaag gtggtgaaga
     5401 aggcgaagag ccaggccagc catttctgtc ccgtggcttt ctccagaacg tgcattgtgc
     5461 cgccgcgcca gttccctcct tcgtccttct cacgatacct gacggcgaga gcagcctcgg
     5521 tcatcttggt ggtcatcccg aagatggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnntg atctcagttg tcatacagga acgatttgat taaatctcct ggtaaaatta
     5701 taccataaag ggctgctctt gttttcatga actgtccttt ccaactaaag gaagccttcg
     5761 ctcctgagga tttcctcggc ttcctcgagg accctggctg ggaccttcac aaccggtccc
     5821 gtgcaaccca tggcggattc ggcgtagatt tccctcttcc aaagagtttt gacggcttcc
     5881 tcgatggaaa gaacatcgat gccgtgaatc tcgtcctcgg tgggctcctt gggcggcatc
     5941 ttcacttctc cgcttccatc ggatttcgtt gggcgtaact ccgagaggat gccggaaagt
     6001 ccggcctttt ccgcgagagc caggtattcg atcacctttt ccggaagaga ccccgtcgcg
     6061 gccgcggcgg aaaacaccag cgccccggca atgaccggag ctcccgaggc cctggatatg
     6121 atcgagacta taaagggcca tcctctccca acggagggcc cgtaacccca ccccgtagcc
     6181 tcgtacccgc ctccggtcgt aaaggccgag aacatcttga tgagcacgtt gcctgtcagt
     6241 gtgtcggtga cgcagacatc gacagcgccg gcgagaatat cgttgcccct gagtatcgcc
     6301 cccccatctt tcctgacgct gctcccgaag gttatgggga atccgttgtc gcgaatcttc
     6361 tggagggctc tgaaggctgc ctgggcgcct tcgatattaa gtagccccac ggtgggctcg
     6421 gttatgccga gcgacttcgc cgtggcaatt ccgtaaaccg cgttcttgac catggcctcg
     6481 atcctctccg tggcgctggt tccggtcgtg gaggctatga acatcggcct tccccgcgca
     6541 ggagtcacaa ccctccctat ggtcgtcacc ccaaggggga aggggtaatg gagggcgacg
     6601 gccgcgctga tatcgccttc cttcagtgcc gattccatgg cccgggagat gtcctcctcg
     6661 caatcgggcg tttcgatcca cctcagctgg tcaaaacctt cgatcctcgg tccgatcatc
     6721 accacttcaa ggcctggcct cgattcctgg gccgcgaccg ctccccgggc gagttccccg
     6781 ggaccgagct cgctccctga ggccatgagc cctaccctcg ttcgcggccc cccgcttttg
     6841 gcggactcca ctatttcacg gagggcgtcg ccgatgatcc ttctagtttc cgtcccggtc
     6901 atggccttca tcccgttaat tccttcgaaa ggtcctccag gacttccagg agtattcccc
     6961 ggacctcctc cgaagtgacc cccctggttt cgccggcctt ctccgaggag ggctcgatga
     7021 tgaaggaagc accgtcggat aggttcgtca gcctggcaag gaagaggctt ccctttccta
     7081 ttatcatgac gcgaccgatt ttcccttcct tgatggcctc gcaggcatgg ccgatgaaag
     7141 ggaccccgga aggaatatga ccctgggtat gggcgaaacc cgggataccc ttgctctcga
     7201 caaaggagga catctcatcc ttgccgagct cgcccttcat gacggcgagg gcagctatca
     7261 tcttgaagtt ggccgagggg acgtctccag cgccggccgg tacggtaatc tccggtattt
     7321 ggagttcggc cgcgaacttg tcgacgtcgc caaaggagag ccctatcttc tggagaggtt
     7381 catagaccag tgccgatgtg atcgcctggg gggaggcgcc ggcgcccacc gagtgtttcc
     7441 cgagggcatc gagtctcatg atggggcacg tgccatcgtc ggcatccaaa agaacggcga
     7501 agctccccag gcagtcttcc aaggcaggga tgtccttccg gacgtggtcc cggctgttca
     7561 tgaaaagctt tggaacggct ccgcccgcca ggactaccac gttgggcctc gccctgctgg
     7621 cgacctgggt ggccccagct attacggcgt taacgggccc cgcacagaaa ctccgcacat
     7681 cgaagcccga ggcgtttacg catcctgcta tctccgcaat ggccttggcg aagttccccc
     7741 cgccccgctg gttcatgtca cccgcggctt cctcggagca ttcgatgatg tagtcgatgt
     7801 cggccgggga gaggccggtg gtttttaaaa ggtgaagaag agccagcacg gatccggctt
     7861 tgctggcaat attttcaaga agaacgtgcg cggagagtga ttcgtcgaga tcgtgcccct
     7921 tcctgcagca accagccact ctgccctggg agtaaagggg gagggcaccg tgttctttcg
     7981 tttccttctc gatctctgaa gaggcacgac ctttttccaa ccgcgtcagg atcttctcgt
     8041 tcatgagcgg gtgcctggcc atgacttcgc ggacttcacc ggcgaaggac tcttcgagcc
     8101 agatgatatc gaagacatcg cagatatcca tcaagccgag gaactccttc tcgggcatga
     8161 tctccccgaa tttgccgtac cgcgaggcgc catcgatcct gttacagtac atcggagcgg
     8221 gctgcctctt caggtcatcc aggtccatcc cccctatata ggcctggttg ggggcgtagg
     8281 cacatgcttc ttcaaaggac tgcgtgcttt tacacagttt ttttatgtac tccgtctctc
     8341 cgtgggtgat cctctcaatg tacggggtga ccccgtagtg aagccccagt tccggggtgt
     8401 ggttaagaca gtaggaataa cccctgatag cgacacgggc catgggccgc tcctccctcc
     8461 gtttctatag acgggtggcc cggataagga tccgggccac ccctgggtac tcaatcgtta
     8521 aaaaccgtct ggtcctctac gggcgtctgg agagccttga gggctttctc cacgatggcc
     8581 cgcctgatct tcttctcctc ctcgggggtc ttcgtcgggt cccccagggg gtgagggatg
     8641 gccaccgcag gcacgatcct gttcgctccg acggtgagtg aaatgggcac tatggtgcag
     8701 acatggacta cgggaagccc tgccctttcg atctccttga cgatcgttgc gccgcaacga
     8761 gtgcaggtcc ctcaggtgga ggtcactatc acagcgtcca cgttggcgtc cttgagtttc
     8821 tgggcgatct ccgtcccaaa acgcctggcg ttgcccacgg aggtgccgtt gccgacggtc
     8881 gcatagaagg tctcgtgaaa tttcttgaag gcaccctctt tttcaaggtc acgcatcacg
     8941 tcgacgggta ggaccctgtt ggggtcgtcg ttcgcgtagg tggggtcgta gccgccatgg
     9001 gccgtctcat gggtggaggc gtctattttc atgacgccgg ccaggctgta ttctccgtac
     9061 ttcgaagcgc tcgaagcctc gatgtggtcg gggttgccct tcggtacgat gccgccggag
     9121 gtcacgaggg caatggtcgc gctcttcatg tcagccaccg gcgggttagg aggcaccctg
     9181 tcaaaggtcg gcatggggta ttccgtcgtg aagggctccc ccttgagctt cttcacgaac
     9241 atatccacgg ccctctcggc cccgatcttg tccatgaaga agttcaccct tacgcctctt
     9301 tcgatgtagc cctcatcatc gggcttgcct atcggctcgc ccttgagttg cttgagaaga
     9361 aggcgggcca tggcgggaac cgcatccttc atgcccctgg cgctgtcagc cgtcctggcg
     9421 atgtaagtcg acttacggta catgtcaacg ccggggttct cctcgtacat tcctgatact
     9481 ctcggcaggc cgagagtctt tcccaccgct tcgcagacgg cgccacaggc tgtcccgtaa
     9541 cggccggcgt tgaaggccgg tccggcgacg accgcatcgg gagaaaagga cgcgatgagg
     9601 tccaggacct ccttctcggc cttttcaagg ttgtcggcaa agtaggtgtc cccgcagatc
     9661 accgttccca cgatctccgc gtcaggggcc aaggccgcgt tgaaagcctg gccgggtccc
     9721 acgaaaccct ttctggattc cggtctgtgg ccggccatct cctccccgcc gatgcctgcg
     9781 aagaactggt tgatataatg aactaccttg tacttcataa gatcatttcc cccctttctt
     9841 cggggctacc ctctagaggg tgtacttccc gtactgttcg cgaaccccct tgacggcgtt
     9901 ggcgaggctc tcggtctcaa ggaccatctc catcataccg atctggtcgg cccagaccga
     9961 tgggtcggcc tcgtcccgga tctcctgttc gaaaacgtgg tataccgcga gtcccaacgg
    10021 gactcctgcc agtggacctg cgaaggtggg atcgccgttt gtcaccgttt ccgcgtatat
    10081 ttcggctccc tcggcgtcgg aagaacccag gatcactacc aggttctccg ccccgaactt
    10141 ctcagccgcg tccttgacgc gctgctgatt ttgcaggtcc atggcccccg cggcggttca
    10201 gacgaagcac tccgtcacgg agaagacaat atccgcaccg caatttttca ggctctcctc
    10261 catagcgggg gcggccactc catccctctc gcccagaagg agcaccttct tgccagcgag
    10321 tttgcccaaa tcaggcacct ccttttaaag tcaaagattc ttgaagcaga aacctagata
    10381 gtgtaggcgc cgatccggct gaagccgagc tcgctggtcg ccccggttat cgcctggatc
    10441 tccacctcga tggaaccgtc ctccctcagg gaaccctgga aacccccggc gatcacattg
    10501 gccgtgtccg ggagcccaat tatcttctcc atccttggca gcacgatgat ctcgttggcg
    10561 ttggctgccg tcacgacggc atcggcttca gggcaggcgt cggccagaga ctggctggcc
    10621 ccgtccctgc ccgcgtattc gtcagttacc aggacagtct tgatcccact ctgctcagcc
    10681 ttgcggcagt tcatgatcag gtccgtatcg gggttgccga agccttcctc gctgatgatc
    10741 acgccctcga caccaagcat cttggccagt ttcacggcat aggatgaact gcgcttcttg
    10801 tcggcgaggg tggtattctc gtttgtgatg acacagccga caaagttgag atccttgcca
    10861 tgtctcttgt acatgtgatg gattatcggg ttgttctggt gagaataggt attgttcttg
    10921 tcgcaggcgg aaacacagtt cccggagacg atcgccccgt ccatgacctc gttggggtgg
    10981 ataagggtcg ggatgatcct cttggcatcg acaccgtaga cataggtgtc gtgtagaagc
    11041 ccctgggact gcagcatgta caggtaggcc actttcggga gctccgggaa gaacgtcatg
    11101 gccttggcaa gatccggcag gcaataggtt tcgacttctc ccccggtcgc gcccttggcg
    11161 gcggtcgcga ggtagtgtgc cgtcttcaag ccggcgagcc ggcaagcgga ctcgtattca
    11221 tgcttttcaa ggccttcgac gggttcgacg acgagcacca cgttaagggt cttggaaaag
    11281 ggcgtgtatt ccgctcccgg acccgacatg tcgatgatgc cttcctggaa acccacgatc
    11341 tttccgcagg tgaccacggc ggcgccgacg agggcgagag tggtgccttc gcccaccgtc
    11401 tcgacgtcgc cgatgaagcc cgggaacact tctcccggcc cttcaagctt gcacctcggc
    11461 tctaccacgt ccttgaccgg caagatcctc gtgctctccc ccggcagggc aagctcaacc
    11521 atggcgctgg agaacctctt gtccttcatc agcatgttga cgatctcccg tcggttcacc
    11581 tttaggaccc cgtcatcaaa ggaagtcttg tctccgtcca aaagggacct tacgactact
    11641 ttcttgagct taagttccaa ttatgtttcc cccctttcaa atacccatcg ctatccggac
    11701 aattctttaa ccttctcctc gatcttgtca gccactgcct cggctccgct caacctgggg
    11761 ccactctcct ttcccccctt gtagaacagg aaggtcggca cccccatgac cctgagggat
    11821 atgacgagct tgcgattctc ggcgacgttg agcttgcaga acttgacctt gccttcatac
    11881 ttgcgggata tctcctccac cttcggcatc aaagccaggc aggggccgca ctgcggaccc
    11941 caaaggtcaa ccagcacagg aaggtcgctc ttcaggacct cttcttcgaa gttttccttg
    12001 ttcagttcga tcatcttttc acctcccttc agctttctcc gcccttcggg gttttacggc
    12061 cgcttcagga accatcacag gcgctactcc ttcagcaagc cgtatctctc catgctgtcc
    12121 cgcaggaggt atacatccag ggcggcaaag tcctccaggt ggcggtcccc cctggctggc
    12181 ttcacggagt ttactccctc cgccctgtaa agaccgatgg ctctctcaag gatagaaatc
    12241 gatccaaggt cgccctccct tttttcgtcg ggcaggccga caaggacgct cctgaagtgt
    12301 tgctgggagg gctggatatt tccataaagg ggatgggtca aaagcagcca cccggcatgg
    12361 acgcggtctc tcgcgagggt cagtacgcta agggggccac cctcaacgaa ggtggctttc
    12421 agggaaatgt tccagagccg ggcgttattt gtgaggcagt cgatctcctg gaacaccaaa
    12481 aaacccccta ttcatcgaat aggtgggttc tctgtccaga aattcccgca cggaaggaca
    12541 ctgacgccgc aagggtctcc cgcgcctgtc cagagacttg tccgaccgca atcctttggc
    12601 ctgagagttt cgcgctcctg tcggtcgctt gccccttcgg
//
LOCUS       DAOSRG010000019        12516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5052_length_12516_cov_10.607335, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000019 DAOSRG010000000
VERSION     DAOSRG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5052_length_12516_cov_10.607335"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLM30_01555"
     CDS             complement(<1..1001)
                     /locus_tag="PLM30_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOR53886.1"
                     /translation="MDLNKLTLKSQEALSSAQNIAVTMGHTEVDGEHLLLSLLRQEGG
                     LIPRLLTRMDAHPEEIALEAERELSRRSRVSGPGVEPGKIHLSQRLSKLLVFAQEDAS
                     RLKDEYVSVEHLFMRLLEEGDASAAGRALARAGITPQAFLAVLQDVRGSQRVQSADPE
                     ATYEALERYGSDLVALARSGKLDPVIGRDEEIRRVVRILSRKTKNNPVLIGEPGVGKT
                     AIVEGLAQRIVRGDVPEGLKDRSVFALDMGALLAGAKYRGEFEERLKAVLNEIKESDG
                     RILLFIDELHTIVGAGKAEGAIDAGNMLKPMLARGELHCIGATTVDEYRKHIEKDAAL
                     "
     gene            complement(1041..1994)
                     /locus_tag="PLM30_01560"
     CDS             complement(1041..1994)
                     /locus_tag="PLM30_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOR53887.1"
                     /translation="MKFRDYYEILGVPRDASQKDIQKAYRKLARKYHPDVNKDADAEN
                     RFKEINEAYEVLRDAEKRKKYDTLGANWKNGQDFSPPPGWEGSFQFGGGGGGAFSDFF
                     EALFGNLGGFTTMEGQPFRGRSLRRRGRDLESKIELTLDELAAGGKKRVEFEVLGRGP
                     DGRAKRSRKSLEVTIPKGVTQGSRIRLAGQGEAGSGGGPAGDLYLEVHIKKDPCFEVE
                     GQDLRTTVDIAPWDAALGGSVQVPVLGGSVTMKIPTGTQSGQVLRIRGKGLPGRGDCP
                     GNILVTVRIAVPRKLTDRERELFEALRSESDFKPETKKRWE"
     gene            complement(2071..3957)
                     /gene="dnaK"
                     /locus_tag="PLM30_01565"
     CDS             complement(2071..3957)
                     /gene="dnaK"
                     /locus_tag="PLM30_01565"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOR53888.1"
                     /translation="MSRTVGIDLGTTNSVVAVLEGGHPTVIPNAEGSRTTPSVVGFTK
                     DGERLVGILARRQAVLNPKGTVSSIKRFTGRNYDEVGEEMKIVPYSVVKGNNNSVRVE
                     VDRKQYAPEEISAQVLRKLAQDASSWLGEKVTDAVITVPAYFNDAQRQATKNAGKIAG
                     LNVLRVVNEPTAAALAYGMDKKNNETVMVFDLGGGTFDVSILDVGEGLCEVRSTSGDT
                     HLGGDDFDKRVVDWLADEFKNHEGIDLRNDRQALQRLYEAAEKAKCELSSRTSTDISL
                     PFITADANGPKHLSSNLTRAKFDELTRHLVERTLEPMKQALKDAKLEAGDIDEVIMVG
                     GSTRIPAVQELVKRFTGGKNLNMTVNPDEVVAVGAAVQAGIIKGEVKDVLLLDVTPLS
                     LGIETLGGVMTKIIERNTTIPARRSEVFSTAEDNQPAVDVVVLQGEREMARDNRKLGN
                     FKLEGIRPAPRGIPQIEVTFDIDANGILNVSARDKETGKEQKIAISNSTNLDESEINR
                     MVDDARLHAEEDKKRRGWVDARNELEAASYQVERMIGELGDRAPAHEKARGEELVREA
                     KEAISKEEETPRLQSLTQDLKQAAAALAQHEGPSPHEETGGPAPEGGPGKEEDVIDAE
                     FNEG"
     gene            complement(4209..4610)
                     /locus_tag="PLM30_01570"
     CDS             complement(4209..4610)
                     /locus_tag="PLM30_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53889.1"
                     /translation="MKKRAVLLALAALLTIGTAAYAYGGPYGRFGPGMDGASYGRFGA
                     GMGGGAFCGPRMSSRGGYFRDRDTKRVDYPKEILDKIGELQRTHLEMRLALTQEKPDL
                     EKARSLFSRAQEIRNEISLWRFESYIESLKK"
     gene            complement(4716..>6045)
                     /locus_tag="PLM30_01575"
     CDS             complement(4716..>6045)
                     /locus_tag="PLM30_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOR53890.1"
                     /translation="LAREAGIQMVDFNEIAGDSLSVAARYVRSYRQLPLSFASGNPRR
                     IDACGFAAGAGEAALQRDGFLDTARKICREAGLSPRDGRTIEEARRYVALAVDKGSWH
                     SKEGFVCSSCDWCGDDAAPANPTVRPSGEEAPLEEVETPGADTIAELCRQLGITPDRT
                     LKTLFYAAEQGADKEILAVLTRGDHQVNDRKLSHALGGVVVRFADPLEIREAVGDLAG
                     YLGPVGLPAGIRVLADRHIEGSSRLVAGANRPGFHLLNACWGRDYRASDTADLVSLQE
                     GFPCPLCGSPIAASYLRSVAVAVTDPGPSPLTFQGEGGALSRGHRWEGTFCVTPLALS
                     LAGGERIPPRFAPLDVNVLVTSMRNDDAVGLGNILAERLEEKGFRVLLDDRDERAGVK
                     FSDAELIGLPVTIVVGREASEGVVEAWKPDGNREELPADAVVELVSHWSR"
     assembly_gap    6046..6145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6217..6777
                     /locus_tag="PLM30_01580"
     CDS             6217..6777
                     /locus_tag="PLM30_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53891.1"
                     /translation="MVLNEKGMIVLAAHSPEPEILKGAHPEESFRTGFLHEVLEVLSE
                     LQQGGMIDEFFLMPGFGFDLGVFIGREGKTRSAFFNLKMYMGAKPRVVEIGDQSGSGS
                     EVELLQLNTARSSLAAESFRWVLVDITKPRGNRRYSIFTTDQAREGLMGGLNKKKQNS
                     IKLASVMTFPMTWDELSGKLADFLAR"
     gene            6782..7282
                     /locus_tag="PLM30_01585"
     CDS             6782..7282
                     /locus_tag="PLM30_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53892.1"
                     /translation="MDPLALLGRLLGRRRPPLTLKDMAERAPRLGEYFERLKGKRVLV
                     FNPPFWGFHDIFVDREGGVLLVALKAEGDSFAFIGDERGASLMLKYGPGPVLNAEEDL
                     APGLLEWVLYDDFIVYRGPFFPMSRDPYHLGRVAALADFDGEVEREAVPAEITRLREW
                     YRKRKQ"
     gene            7359..7865
                     /locus_tag="PLM30_01590"
     CDS             7359..7865
                     /locus_tag="PLM30_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR53893.1"
                     /translation="MGRFGIREGTPGDVPVILRFIRELAEYEGIGHRVTATEEGLLET
                     LFGEAPAAETLLASVGGEPVGFAVFYETFSTVLGKRGLHLDDLYISPKWRGKGLGTAL
                     LRHLAAIAVRRGCGRFEWWCMKDNAPALEFYDRIGALKHDEVFILRLQGEAIAAFAGG
                     EAPQGSPS"
     gene            complement(7866..8297)
                     /locus_tag="PLM30_01595"
     CDS             complement(7866..8297)
                     /locus_tag="PLM30_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOR53894.1"
                     /translation="MAGKKPRRVPVDGSSEEVPESVLGGLLRELGFPGPDEGPRKATP
                     PGPGAAPAPEDGVPDLAGVGKIVLRVQRKGRGGKTVTTLSTPGGGLLPRALEPLAREI
                     RRALGCGARVEDGSVVIQGDNAERAEKWLLQRGARRVVRGS"
     gene            complement(8314..8499)
                     /locus_tag="PLM30_01600"
     CDS             complement(8314..8499)
                     /locus_tag="PLM30_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53895.1"
                     /translation="MVAATVRGGAPGDTAGFEAVEGEGFLLWVDRSLQFRGEGIDLDV
                     GFAGSRKVVYATNYISR"
     gene            complement(8506..8676)
                     /locus_tag="PLM30_01605"
     CDS             complement(8506..8676)
                     /locus_tag="PLM30_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53896.1"
                     /translation="MKKGYLVMAAFLAVAAFAAGHAAAAEVELRVTPEAQAEMANLGS
                     VLTLAPETLSSG"
     gene            complement(8711..>8932)
                     /locus_tag="PLM30_01610"
     CDS             complement(8711..>8932)
                     /locus_tag="PLM30_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOR53897.1"
                     /translation="GGRPARDPYEVLGCSPGDSDEKIRKAYRRLVAKYHPDKFIGLDL
                     DKEFVDLAAKRFQEVQEAYEHIRRYRGSL"
     assembly_gap    8933..9032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9159..11030)
                     /gene="htpG"
                     /locus_tag="PLM30_01615"
     CDS             complement(9159..11030)
                     /gene="htpG"
                     /locus_tag="PLM30_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOR53898.1"
                     /translation="MPEKETFQFQAEARQLLDLMIHSVYSNRDIFLRELVSNASDALD
                     KLRFEALTDEDLAPLAEDLHIHIEVDPGARTLTVSDNGIGMTKAELVDFIGTIARSGT
                     REYLELLRGKGAGSFPEELIGQFGIGFYSSFMVADRVSLVTRRAGEAAAWKWESAGDG
                     NYTLEETSRDAQGTSVTLHLKKDEEDETARDYSDPPVIGEIIRRYSDFVAWPIRMKSA
                     DGSLETLNSMKALWTRPEEEVTGEEYDEFYRHITHDWNKPLRRVLFKAEGGMEFRALL
                     YIPPRAPLELFTRDLFKGIQLYIRRVFIMDDCRELVPEYLRFLRGLVDSEDLPLNISR
                     EMLQQNRQVETIKRSIARKVLDSLSAMLKEDRDAYLAFWENFGKVLKEGIFSDGKNRA
                     RILEVSLFDTTGPDGPVTLDEYLARMPEGQEEIYYITAPSLTVGKNAPHLEAFTEKGY
                     EVIVLSDPVDEIWSPAVEDYKGKKFTSLSRGAVEVPSSAGTSEAGATGEGLEALLEDL
                     GKALSGEVKEVRLSGRLKASPSCLVGEAFDLTPQMEAFLRAAGQEVPRVKRNLEINPS
                     HPLVMKLKGIHDSDPSDPRVPRFARVLFGLATLSEGGGLDDPAAFSREVAELLSEPV"
     gene            complement(11172..11597)
                     /locus_tag="PLM30_01620"
     CDS             complement(11172..11597)
                     /locus_tag="PLM30_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR53899.1"
                     /translation="MRQLIPFGRGMGIFDEMDDLFRGFSDAIRPMTRRYVPVDLYEEN
                     DEVVVNIDAPGFDPSQIEIKTYSDRVNVSSKVEEKAEEESGKTWYMRRGERTMNLCVT
                     LPTEVDPERSQASFKNGVISLRLPKSKAVQGRVLELKQE"
     gene            complement(11778..12479)
                     /locus_tag="PLM30_01625"
     CDS             complement(11778..12479)
                     /locus_tag="PLM30_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOR53900.1"
                     /translation="MKTIGLIGGLTWESTIEYYRIINEEVARRLGGVHSARILMHSFD
                     FQDIDTLMLAGRWEEVGAKAAEAGLGLESLGADFLLICSNTIHKVAEAVSATVSVPLI
                     HLADVTAEAVKEKGIGRVGLLGTVFTMEHDFYKGRLAQKHGLEVLIPEKEDRAFVNRV
                     IDEELTLGELKEASRARFLGIIDDLARRGAGGVILGCTEIPLLVKQPDTAVTLFDTTR
                     LHSLAAVEEALKGDS"
BASE COUNT         1933 a         4026 c         3875 g         2482 t
ORIGIN      
        1 agggcggcgt ctttctcgat gtgcttgcgg tactcgtcga cggtggtggc gccgatgcag
       61 tgcagttcgc ccctggcgag catgggcttg agcatgttgc cggcgtcgat ggccccttcg
      121 gccttgccgg cgccgacgat ggtgtgcagt tcatctatga agagcaggat gcggccgtcg
      181 ctctccttga tttcgttcag cacggccttg aggcgctcct cgaactcgcc cctgtacttg
      241 gcccccgcca ggagggcccc catgtcgagg gcgaagacgg aacggtcctt gagcccctcg
      301 gggacgtccc cccggacgat gcgctgggcg agcccctcca cgatggctgt cttgcccacg
      361 ccgggttcgc cgatgaggac ggggttgttc ttcgtcttgc ggctcagtat cctgaccacc
      421 ctgcggatct cctcgtcgcg gccgatgacg gggtcgagct ttcccgacct cgccagggcc
      481 accaggtcgc tgccgtacct ctccagggcc tcgtaggtgg cctcggggtc ggcgctctgg
      541 actctttggc tgccccttac atcctgcagg acggcgagga aggcctgggg cgttatgccg
      601 gccctggcga gggccctacc cgcggcggag gcgtcgccct cctcgaggag cctcatgaac
      661 aggtgctcca ccgagacgta ctcgtccttc aaccgggagg cgtcctcctg ggcgaagacg
      721 agaagcttcg aaagccgctg ggagaggtgg atcttccccg gctcgacgcc ggggccggag
      781 accctgctcc tgcgggagag ctccctctca gcttctagcg ctatctcctc cgggtgggcg
      841 tccatcctgg tcaggagccg ggggatcagc ccgccctcct ggcgcaggag cgaaagtagc
      901 aggtgttccc cgtcgacctc ggtgtggccc atggtcacgg cgatgttctg ggcgctcgat
      961 agcgcctcct gggacttgag ggtcagtttg ttcagatcca tggacaacct cctccagcgg
     1021 ggtcaggtct acacttttga ctattcccag cgcttcttgg tctccggctt gaaatccgac
     1081 tcgctcctca acgcctcgaa gagctcccgt tccctgtcgg tgagcttcct gggcacggcg
     1141 atcctcacgg tgaccagtat gttcccgggg cagtcccccc tgccgggcag gcccttgccc
     1201 ctgatcctca gcacctggcc gctctgggtc cccgtcggga tcttcatcgt caccgaaccg
     1261 cccaggacgg ggacctggac gctcccgccg agggccgcgt cccagggggc gatgtccacg
     1321 gtggtgcgaa ggtcctggcc ctcgacctcg aagcaggggt ccttctttat gtggacctcc
     1381 aggtagaggt caccggcggg gccgccaccg ctcccggcct cgccctggcc ggccaggcgt
     1441 atcctcgagc cctgggtcac ccccttggga atggtcacct cgagggactt gcggctcctt
     1501 ttggcccgcc cgtcgggacc ccggccgagg acttcgaact cgacccgttt cttccccccg
     1561 gcggccagct catcgagggt cagctcgatc ttcgactcca ggtcgcgccc ccggcggcgg
     1621 agggaacggc cgcggaaggg ctgtccttcc atggtcgtga aaccccccag gttgccgaaa
     1681 agggcctcga agaagtcgct gaaagccccg ccgcccccgc cgccgaactg gaaggacccc
     1741 tcccagcccg ggggagggga gaagtcctgc ccgttcttcc agttggcccc gagggtgtcg
     1801 tactttttcc tcttttcggc gtcgcggagc acctcgtagg cctcgttgat ctccttgaag
     1861 cggttctccg cgtcggcatc cttgttgacg tcagggtggt acttcctcgc gagtttcctg
     1921 tatgccttct gtatgtcctt ctgcgatgcg tccctcggca caccgaggat ctcgtagtaa
     1981 tccctgaact tcatcgaaac ccctccaccc tgaccggggc gctttttacc tcgcggtcat
     2041 gcctacggca cgcccccttt ttcaacggca tcagccttca ttgaattcgg cgtctattac
     2101 atcctcttcc ttgccgggcc caccctcggg ggctggcccc ccggtctcct catgagggga
     2161 tgggccttca tgctgggcga gcgccgccgc tgcctgcttc aggtcctgcg tcagcgactg
     2221 cagcctgggg gtctcctcct ccttggagat ggcctccttg gcctcccgga cgagttcctc
     2281 gccccgggcc ttttcgtggg ccggggcccg gtcgccgagt tcgcctatca tcctctccac
     2341 ctggtaggac gcggcctcga gttcattcct ggcgtcgacc cagccacgcc tcttcttgtc
     2401 ctcctcggcg tggagccggg cgtcgtcgac catccggttt atctcgctct cgtccaggtt
     2461 cgtcgaattg cttatggcga tcttctgctc cttgccggtc tccttgtccc gggcggacac
     2521 gttgaggatg ccgttggcgt ctatgtcgaa ggtcacctcg atctgcggta tcccccttgg
     2581 ggccggccgg atcccctcca gcttgaagtt gcccagtttc ctgttgtccc tggccatctc
     2641 gcgctcgccc tggagcacga ccacgtccac cgccggctgg ttgtcctcgg ccgtggagaa
     2701 cacctccgac cggcgggccg gtatggtggt gttcctctcg atgatcttcg tcatgacgcc
     2761 gccgagggtc tcgatgccga gggagagggg cgtgacatcc agcaggagca cgtccttgac
     2821 ctcgcccttg attatccccg cctggacggc ggcccccacg gccaccacct cgtcggggtt
     2881 gacggtcatg ttcaggttct tgcccccggt gaaacgcttc accagttcct gcacggcggg
     2941 gatcctcgta gaaccgccca ccatgatgac ctcgtcgata tcccccgcct cgagcttggc
     3001 gtccttcagg gcctgcttca tgggttccag ggtacgctcg acgaggtgcc tcgtgagttc
     3061 gtcgaacttg gccctggtga ggttggacga caggtgcttc gggccgttgg cgtcggcggt
     3121 gatgaagggc aggctgatat cggtgctcgt gcggcttgac agttcgcact tggccttttc
     3181 ggccgcctcg tagagtctct gcagggcctg cctgtcgttc ctgaggtcga tcccctcgtg
     3241 gttcttgaac tcgtcggcga gccagtcgac aacgcgcttg tcaaaatcgt ccccgccgag
     3301 gtgggtatcg cccgaggtgg agcgcacctc gcagaggcct tcgcccacgt caaggatgga
     3361 gacgtcgaag gtcccgccgc ccaggtcgaa gaccatgacg gtctcgttgt tcttcttgtc
     3421 catgccgtag gccagggccg ccgccgtcgg ctcgttgacg acccgcagca cgttcaaccc
     3481 cgctatcttg ccggcgttct tggtggcctg gcgctgggcg tcgttgaagt aggccggtac
     3541 agttatgacc gcgtcggtga ccttttcgcc gagccaggaa gaagcgtcct gggccagctt
     3601 tctgaggacc tgggcgctga tctcctccgg ggcgtactgc tttctgtcca cctcgacgcg
     3661 gaccgagttg ttgttcccct tgacgacgct gtaggggacg atcttcatct cctcgccgac
     3721 ctcgtcgtag ttccttcccg tgaaacgctt gatggaggag acggtcccct tggggttgag
     3781 cacggcctgc ctgcgggcga ggatacccac caggcgttcc ccgtccttcg tgaaacccac
     3841 caccgagggc gtggtcctgc tcccctccgc gttgggtatg accgtgggat ggcccccctc
     3901 gaggacggcc accaccgagt ttgttgtgcc gagatctatc ccgacggtac gagacattaa
     3961 tattccccct tgaggctctc tcgcgggagg acccgcgaaa agcctgacta atgttgacct
     4021 ttaccctgac aattatatag gtcaatattc atcaggcaat atgggaaagg atggtatata
     4081 gcggttaaag gcaaatatac cttgtttctt ttgatttgtc acgtttttcc ccttttttca
     4141 agggatatct gggggaaaat aaaaaacgcg gggccctggg gccccgcgga agggaggagt
     4201 tggtaccgtt atttcttcaa cgattcgatg taactttcaa aacgccaaag ggagatctcg
     4261 ttccttatct cctgagctct ggagaaaagg gacctggcct tttccaggtc cggcttctcc
     4321 tgggtgaggg cgagcctcat ctcgagatgg gtccgctgga gttcgcctat cttgtctagg
     4381 atctccttgg ggtagtcgac cctcttggtg tccctgtccc tgaagtagcc gcctctggag
     4441 gacatccggg ggccgcagaa agcgccgccg cccatcccag cgccgaaccg gccgtaagaa
     4501 gcgccatcca ttccaggtcc gaaccggccg tagggcccgc cgtaggcata agccgccgtg
     4561 ccgatggtca gcagtgccgc gagggccagg agaacagctc tttttttcat cccatccacc
     4621 tccgggtgtt tttctttgaa ccctgttgac gccattatat tcagcgcccc cggcggaggt
     4681 gatgggtaaa atggcttatc ctcccctggg tggttttacc gggaccagtg cgagaccagt
     4741 tccacgacgg cgtcggcggg cagttcctcc ctgttcccgt cgggcttcca tgcctcgacg
     4801 acgccctccg aggcttccct gccgacgacg atggtcacgg ggaggcctat cagttcggcg
     4861 tcggagaatt taacgcccgc cctctcgtcc cggtcgtcca gcaggaccct gaagcccttc
     4921 tcttcgagtc tttcggccag gatgttgccc aggccgacgg cgtcatcgtt tctcatggac
     4981 gtcaccagga cgttcacgtc caggggcgcg aaccggggcg ggatcctttc accccccgcc
     5041 agggagagcg ccaggggggt gacgcagaag gtcccctccc agcggtggcc tcttgaaagg
     5101 gcgccccctt caccctggaa ggtcagcggc gacggcccgg ggtcagtcac ggccacggcg
     5161 acgctcctca ggtaggatgc cgctatgggc gaaccgcaaa gggggcaggg gaagccctcc
     5221 tgcaggctga ccaggtccgc cgtgtcggaa gccctgtagt ccctccccca gcaggcgttg
     5281 agcaggtgga accccggcct gttggccccc gcgaccaggc gggagctgcc ctcgatatgg
     5341 cggtcggcga ggacgcggat gccggcgggg aggccgacgg gtcccaggta gccggccagg
     5401 tcgcccacgg cttccctgat ctcgaggggg tcggcgaagc ggaccacgac gccgccgagg
     5461 gcatgggaca acttccgatc gtttacctgg tgatcgcccc tggtgaggac ggccaggatc
     5521 tccttgtcgg cgccctgttc cgccgcgtag aagagggtct tcagggtccg atcgggggtt
     5581 atgcccagtt gcctgcacag ctcggcgatg gtgtcggccc cgggggtctc gacctcttcc
     5641 aggggcgcct cctcgccgga aggccgaacc gtggggttgg ccggggcggc gtcgtcgccg
     5701 caccagtcac aggaggagca gacaaacccc tccttggagt gccatgaccc cttgtcgacg
     5761 gccagggcga cgtagcgcct cgcctcctcg atggtacgcc cgtcgcgggg ggagaggccc
     5821 gcctcgcggc agatcttccg ggcggtgtcg aggaacccgt cgcgctggag cgccgcttcc
     5881 cccgcaccgg cagcgaaacc gcaggcgtcg atcctcctgg ggtttcccga cgcgaaggag
     5941 agggggagct gccggtagct cctgacgtac ctggcggcga ctgaaaggga gtcacccgct
     6001 atttcgttga agtcgaccat ctggatgccg gcctcccggg cgaggnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnccccc cttgggttat aatcggattc acttggactg
     6181 gacaggctcc cggagaacgc ccccggagtg gggggcatgg tcctaaacga aaaggggatg
     6241 atcgtcttgg cggcacacag ccctgagccc gagatcctga agggagccca cccggaggag
     6301 agcttccgca cgggcttcct gcacgaggtc ctcgaggtgc tctctgaact gcagcagggc
     6361 ggcatgatcg acgagttctt cctgatgccc ggcttcggct tcgacctggg cgttttcatc
     6421 ggacgggagg ggaagacccg gtcggccttc ttcaacctca agatgtacat gggggcgaaa
     6481 ccgagggtcg tcgagatagg cgaccagagc ggctcggggt cggaggtgga actgctgcag
     6541 ctcaacaccg ccaggagctc cctcgcagcg gagtccttcc ggtgggtcct ggtggatatc
     6601 accaagccca gggggaaccg caggtacagc atcttcacca ccgaccaggc aagggagggc
     6661 ctcatggggg gcctgaacaa gaaaaagcag aacagcataa agctggcttc ggtgatgacc
     6721 tttccgatga cctgggacga gctgtcgggc aagctcgccg acttcctggc ccgttgatct
     6781 catggacccc ctggcgctgc tggggcgtct cctcgggagg aggaggccgc ccctcaccct
     6841 caaggacatg gccgagaggg ctccgcgcct gggggaatac ttcgaaaggc ttaagggaaa
     6901 gagggtactg gtcttcaatc cccccttctg gggcttccac gatatcttcg tggaccggga
     6961 ggggggcgtt ttgttggtgg cccttaaagc cgaaggcgac tccttcgcct tcatcggcga
     7021 cgagcgcggg gcgtcgctga tgctgaagta cgggcccggg ccggttttga acgccgagga
     7081 agacctcgcg ccgggtctcc tcgaatgggt cctttacgac gacttcatcg tctaccgtgg
     7141 acccttcttc cccatgagcc gcgaccccta ccacctgggc cgggtcgcgg ccctggcgga
     7201 cttcgatggt gaggtggaga gggaggccgt cccggcggag atcacccgtc tgagggaatg
     7261 gtacaggaag cggaaacaat gaggttcggc ggtgaggaag gttcggcgat cctgaaatga
     7321 cctgtgaggt atgacccttg ggggaagggg cggagtcgat gggccgtttc ggcattcggg
     7381 aggggacgcc cggcgatgtg ccggtgatcc tgcggttcat cagggaactg gcggaatacg
     7441 aggggatagg acacagggtg accgccaccg aggaagggct cctggagacg ctcttcggcg
     7501 aagcgccggc ggcggagacc ctgctggcat cggtgggagg ggagcccgtg ggtttcgccg
     7561 tcttctacga gaccttctcc acggtcctgg gcaagagggg gctccacctg gacgacctct
     7621 acataagccc gaagtggcgc ggcaagggcc tgggaacggc tctgctccgc cacctggcgg
     7681 cgatcgccgt ccggcggggg tgcgggaggt tcgagtggtg gtgcatgaag gacaacgccc
     7741 cggccctgga gttctacgac aggatcgggg cgctgaagca cgacgaggtg ttcatattga
     7801 ggctgcaggg ggaggcgatc gcggccttcg ccgggggaga agcgccccag gggtccccct
     7861 cgtgatcagg aacccctgac gaccctcctc gcccctctct ggaggagcca cttctcggcc
     7921 cgctcggcgt tgtcaccctg gatgaccacc gacccgtcct cgaccctggc cccgcagccc
     7981 agggccctgc ggatctcccg ggcgaggggc tccagcgccc tcggcaaaag accgcctcca
     8041 ggggtggaga gggttgtcac ggtcttaccg cccctcccct ttctctggac cctgaggacg
     8101 atcttgccca caccggccag gtccgggacc ccgtcttccg gggctggggc cgcccccggc
     8161 ccgggcggcg tggccttccg cgggccctcg tcggggccgg gaaaccccag ctcccgcagg
     8221 aggccgccca ggacgctctc agggacctcc tcgcttgagc cgtcgacggg gaccctcctg
     8281 ggttttttgc cggccatggg caacacctac cgctcaccgg gagatatagt tcgtggcgta
     8341 gacgaccttc ctgctgccgg cgaagcccac gtcgaggtcg atgccctcac cccgaaattg
     8401 aagggaacgg tccacccata gcaggaaccc ctcgccctcg acggcctcaa agcccgccgt
     8461 gtcccccggg gcgccgccac ggaccgtggc ggcaaccacc ctggctcagc cgctggaaag
     8521 ggtctccggt gccagggtca gtacgctccc caggttggcc atctcggcct gggcttccgg
     8581 tgtgaccctc agttcaacct cggcggccgc ggcatgaccg gccgcgaagg ccgccaccgc
     8641 caggaacgcc gccatgacca ggtatccttt cttcatccca tcgacactcc tctctcttgg
     8701 gaaagcgccc tcaaagggat cccctgtacc gcctgatatg ctcgtaggcc tcctggacct
     8761 cctggaacct cttggcggcg aggtccacaa actccttgtc caggtcgagg ccgatgaact
     8821 tgtcggggtg gtacttggct accagcctcc ggtaggcttt cctgatcttc tcgtcgctgt
     8881 cgcccggcga gcagcccagg acctcgtagg ggtcccgggc gggcctcccc ccnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttctctc ccgctcgagc ctctcgcggt
     9061 tgagacgaac ctcgctgtcc tcatcgacga acgccctgga cacggagtcc acctcccccg
     9121 gggatgttga tgaccatgcc ggcggcacgg ccagttaatt ataccggctc cgacagcagt
     9181 tcggcgacct cgcggctgaa ggccgccggg tcgtccagcc ctcccccctc ggagagggtc
     9241 gcgaggccga agaggaccct ggcgaacctg gggacccttg ggtcggaggg gtcggaatca
     9301 tggatgccct tcagtttcat caccagggga tgggaggggt ttatctccag gttcctcttc
     9361 accctgggga cctcctgccc cgccgccctg aggaaggctt ccatctgggg cgtgaggtcg
     9421 aaagcctcac ccacgaggca cgacggcgaa gccttcaggc ggccggagag tctcacttcc
     9481 ttcacttccc ccgagagggc cttgcccagg tcctccagga gggcctccag gccttcaccg
     9541 gtggcgccgg cctcactcgt ccccgccgac gaggggactt cgacggcccc cctcgagagg
     9601 gaggtgaact tcttgccctt gtagtcctcc accgccgggg accatatttc gtccacgggg
     9661 tcgctgagca cgataacttc gtatcccttt tcggtgaagg cttccaggtg cggggcgttt
     9721 ttgcccaccg tcaggctcgg cgccgtgatg tagtagatct cctcctggcc ttcgggcatc
     9781 ctggccaggt actcgtcgag ggtcacgggg ccgtcgggcc ctgtcgtgtc gaagagggag
     9841 acctcgagga tcctggcgcg gttcttcccg tcggagaaga tcccctcctt gaggaccttt
     9901 ccgaagttct cccagaaggc caggtaggcg tccctgtcct ccttcaacat cgccgagagg
     9961 gaatcgagga ccttcctggc gatgctcctc tttatggtct ccacctgccg gttctgctgg
    10021 agcatctccc tggatatgtt gaggggcagg tcctcggagt ccacgaggcc ccggaggaac
    10081 cggaggtact cgggcacgag ttcgcggcag tcgtccatga tgaagacccg gcggatgtag
    10141 agctggatgc ccttgaagag gtccctcgtg aagagttcca gcggcgccct gggcgggatg
    10201 tacagcagcg ccctgaactc catcccccct tcggccttga aaaggaccct cctgagcggt
    10261 ttgttccagt cgtgggtgat gtgcctgtag aactcgtcgt actcctcccc ggtgacctcc
    10321 tcttcgggcc tcgtccagag ggccttcatg gagttgaggg tctccaggga gccgtcggcg
    10381 gacttcatcc ggatcggcca ggccacgaag tcgctgtaac ggcggattat ctcgccgatc
    10441 accggggggt cggagtagtc cctggcggtc tcgtcctcct cgtccttttt caggtggagc
    10501 gtcaccgaag tcccctgggc gtcgcgggag gtctcctcga gggtgtagtt cccatcgccg
    10561 gcggactccc acttccaggc cgcggcctcg ccggcccggc gcgtcaccag ggagaccctg
    10621 tcggccacca tgaaggagga gtagaacccg atgccgaact ggccgatcag ctcctccggg
    10681 aatgagcccg cgcccttacc ccgcagcagt tccaggtatt ccctcgtgcc cgaccgggcg
    10741 atggtcccga tgaagtccac cagttccgcc ttggtcatgc cgatgccgtt gtcggagacg
    10801 gtcagcgtcc gggccccggg gtcgacctcg atgtggatgt ggaggtcctc cgcgaggggg
    10861 gcgaggtcct catcggtcag ggcctcgaag cgcagcttgt ccagcgcgtc cgaggcgttg
    10921 gacacaagtt ccctgaggaa gatatcgcgg ttggagtaga cggagtggat catcaggtcc
    10981 aggagttgcc tggcctccgc ctggaactgg aaggtctctt tctctggcat caacggatcc
    11041 ctcctgggtt cgcccggctt taccgccggc gtcttttctt tgggttcatc gaaaaaaggg
    11101 cggcgggtcc gccgccgccc caaaacctta tcttacaaaa aaggagcctt caacccgtga
    11161 tagggcgggc cttattcctg cttcagttcc aagacgcggc cctgtaccgc tttgctcttg
    11221 gggagcctca gtgatatgac cccgtttttg aaactggcct gggacctctc ggggtccacc
    11281 tcggtgggaa gggtcacgca gaggttcatg gtcctctcgc ccctcctcat ataccaggtc
    11341 ttgccgctct cttcctcggc cttctcctcg accttggaac tcacgttcac cctgtcggag
    11401 tacgtcttga tctctatctg ggatgggtcg aaaccgggcg cgtcgatatt gacgacaacc
    11461 tcgtcattct cctcgtaaag gtcgacggga acgtatcttc gcgtcatggg cctgatggcg
    11521 tcggagaaac ccctgaagag atcgtccatt tcgtcgaata tgcccatacc cctgccgaac
    11581 ggaatcaatt gtctcatcaa aaaccccctc cttttgctta cctggggaga ggataccaaa
    11641 attcgtcagt aaaggtcaaa tatattagtc agtataggtt agtaattttc ccgcttgaag
    11701 gaaaatatcc cttttttttg accgattccg tccgatcctg ctgttttcca aggattttct
    11761 tcagggtcct ttgacgctca ggagtcccct ttgagggcct cctcgacggc cgccagggag
    11821 tgcagtcgcg tcgtgtcgaa gagggtgacg gccgtgtcgg gctgcttcac cagcaggggg
    11881 atctcggtgc agcccaagat gacccccccg gcgccccggc gagccaggtc atcgatgatc
    11941 cccaggaagc gtgccctcga ggcctccttc aattcaccca gggtgagctc ctcgtcgatg
    12001 accctgttca cgaaggccct gtcctccttt tccgggatca ggacctccag gccgtgtttt
    12061 tgggccaacc gccccttgta gaagtcgtgt tccatcgtga agaccgtccc caagagaccc
    12121 accctgccta tgcccttttc cttgacggcc tcggccgtca cgtcggccag gtggatgagg
    12181 gggaccgaca ccgtcgccga aacggcctcg gccaccttgt ggatggtgtt ggagcagatc
    12241 aggaggaagt cggcgccgag gctttccagc ccgaggccgg cctccgccgc cttggctccg
    12301 acctcctccc aacgccccgc cagcatgagg gtgtcaatat cctggaagtc gaagctgtgc
    12361 atcaggatcc tggcggagtg taccccgccg agcctccggg cgacctcctc gttgatgatg
    12421 cggtagtact cgatggtgga ttcccaggtc agcccgccta tcagtccaat ggttttcatg
    12481 atctgacctc cctgtgcgta aatcgtgaat cgtaag
//
LOCUS       DAOSRG010000020        12434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5090_length_12434_cov_7.441797, whole genome shotgun sequence.
ACCESSION   DAOSRG010000020 DAOSRG010000000
VERSION     DAOSRG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5090_length_12434_cov_7.441797"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..562)
                     /locus_tag="PLM30_01630"
     CDS             complement(101..562)
                     /locus_tag="PLM30_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOR53901.1"
                     /translation="MLIDRVRYYYLLEDMNCAETILCAANDEYSLGLDPVGLRIMAGF
                     GGGMGIGSTCCGALSGSIAAISVKFTSTRSHENPLVEELCRAFLEGFAARHGAIDCGP
                     LKDLYRDSRFGCLPVVELAAEELERSMLENESRVPSPPGAVKRAKDGGSGR"
     gene            complement(600..1442)
                     /locus_tag="PLM30_01635"
     CDS             complement(600..1442)
                     /locus_tag="PLM30_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOR53902.1"
                     /translation="MPSTWQERIISKSKPKGTYRLVPWRICRDAAREAGLPARKVELW
                     ACEQGICPTRYERSLGTLGLEGQARLLASRAAVIGCGGLGGWIVEILARAGVGEIVVV
                     DGDVFGDSNLNRQSLSREDNEGASKALEAEKRIEAINGAVEVTAWSLFLTADNALDIL
                     EGCQVAVDALDSNEARATLLSACRQLGIPLVHGAIGGFWGQACVILPGDRAPWELATG
                     EGKGVEDIAGNPPFTPAFVASLEAAETIRLLSGAGEPLAGMLWCDLKEHEYCKIDLRK
                     RPGG"
     gene            1583..2371
                     /locus_tag="PLM30_01640"
     CDS             1583..2371
                     /locus_tag="PLM30_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR53903.1"
                     /translation="MKRIFVSGASGNVGKLVVRNVLEREGHELAGGWCLEAGRDLGVL
                     AGMAPAGVISSADLASGLRDSSPDVVVDFTSTAVLMNNLRVYADAGVDAVIGTTGLTD
                     GEMAKVGEMVKEKGLRWSVISNYGLGINLVMDFLRTARQYYPYVAVTDRHPARMANAP
                     SGTAATLAGIAAEGPKGEVESREVFPGVMGAMISGVPVTSQRFPYPGPYSEHEVTLAR
                     QDEVIRVTVQDFSSEIYIDGIFLAVGKVGDLPRGTLARSLKELV"
     gene            complement(2377..3978)
                     /locus_tag="PLM30_01645"
     CDS             complement(2377..3978)
                     /locus_tag="PLM30_01645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR53904.1"
                     /translation="MAGTAIGIRGSLWTGDPRRPRAEALVVKGGDIIAVGSYEEVRDG
                     RQPGGVEWIDAGRRTILPGITDSHLHLGALVRQGSALSLYEASSREDLLERVRRKSAE
                     LGPNRWVHGVRFDNSRWPDTALPTLEELDGLGLPNPVLLVRVCTHVQVVNSRALHEAG
                     LSAAGFTGAGVVRDERGRFTGTLLEGSAGPVIRAMERDTAGAARALKETMLSLASEGI
                     TGVHTCSAPSYGLGEDLGAYRVLHERGELPIRVRLYSDEPLPGWESSGQGDEWLCYGG
                     RKFFLDGSLGGRTAAMTFPYEDAPSTRGALNMSDEEFLRQARDEHAAGNQLQVHAIGD
                     AAVDQFIRAMVSLGDLPPLPSGLRHRVVHLQVCRPDQVRDLARLGAVCDVQPAFVPSD
                     IAIAEGRIGRARLGWAYAWKSMLRAGLLLTGSSDAPVEPTNPFRGVWAAVNRVDDEGR
                     PAGGWLPGQRLTLDEALALFTVNAAEAVGQGDRFGSLRPGSRADLVILNRDIDLTPPG
                     ELKDVKPLLTMAGGKITFQAASGGL"
     gene            complement(3994..4872)
                     /locus_tag="PLM30_01650"
     CDS             complement(3994..4872)
                     /locus_tag="PLM30_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOR53905.1"
                     /translation="MGKEGITPEILEKIKVFQRTEITEYHVYKRLSQWMKGKNREVLE
                     KIAQDEKRHAGIWRRYTGESLAPDRLMLYWYLLLAFVFGVTFAIKIMERNEGSAGEAY
                     GAVADVLPVATEIMHEEEEHEKALIDLLDEERLKYIGSMVLGLNDALVELTGALAGFT
                     FALTDGRVVGVAGLITGVAATLSMGASEFLSQRTDSGDLNPLKAAVYTGIAYLVTVAL
                     LVAPFFIVGNVYAALAWSLFNALVVILLFSFFVSVVKETPFRPAFLEMAAISLGVAAI
                     SFLIGLVARHFIPVDI"
     gene            complement(5052..8183)
                     /locus_tag="PLM30_01655"
     CDS             complement(5052..8183)
                     /locus_tag="PLM30_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR53906.1"
                     /translation="MNLAKWSLEHPYLVLALACAVGVLGLAGVMSSPRDLFPDTTPPQ
                     VLVVTVREGASAPEMARTVTEPLEREISGLNGLANQTSTTADGVSSLRAEFTYGKKVS
                     EAVTEVRNALAAVTPRFPAGTAEPLVYSITSRTAPAVTLALSPLDEGGPTLSEIRLLA
                     EHVIRDELLRIPGVGEVEVFGGHHPSVEVSVDRDLLAASGLSLEGLIGAIAKQNISAP
                     AGRLTVEGAEIHFTLDSLFPGVDQVGDTVIASPEGGAVNLRDVARVGIGEMPQRSLYH
                     GNGRPAIAVNVLRAEGSDTIRVVKAVTDLIPELESRFRSMRIEVTDSQKPLIELNISG
                     MYGSLVQAIILIVVVNFLFLGNLAAALVSSVSIALSFLFGLAVIWASPYTIDMVTLTG
                     MIVAVGMVVDASVVVLENIFRVHEATGMKDPRGAAVAGAKEVALSVTAGMLTTVIVLL
                     PVMGSSGYTSRVLRPLNMVLFATLAASLVAALTIIPLLAGFVLEGKGHRPSGVLRKIT
                     SWFDGVLKKVVTFYIELLKVSLRHRGLTVAAFALFFLLTLRLVMPLLGGEMMPPMDTG
                     IAFIEFESPAGRSPEAVEKTLTAMEKKILEEKHVLSLSSQVGSEPGSPSFGGGSTPQA
                     GRIKVLMVDRSKRKENIWEILDRWRADFKSIEGLRWSRLTEYGATPRATTKAPLDVII
                     SGPDAPLLDSLAREAVSAMEGVKGLGDVHQGWFIEKETRNIEVDPYLASRYRTSPTDI
                     AREVGTALNGRTATYLKVPSFSDIPVRVFYGDQYLEKEEDILDVTIVTGDGLLPLSAL
                     ARVRTVPEAPLITRERLRNTIDITGVNRGRTIKHVTSEVKLRLRGIQPPDGYSIEVAG
                     SSADLGESMAMLGKALLLGVVLLYFLLVSLFRSFLYPLTILSIVPPAVAGGLWGLLLF
                     NKSMSMNAAMGFILLAGTVVNNSILLVDFIIESRKRGASRTLAVVRSVKTRTRPILMT
                     VTSTIIGLTPLVMEWAVGLERMSPLGIVAASGLIFGTFVTMIFVPVIYSVIDDISSRA
                     AALVGRTGEDPGA"
     gene            complement(8180..9379)
                     /locus_tag="PLM30_01660"
     CDS             complement(8180..9379)
                     /locus_tag="PLM30_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR53907.1"
                     /translation="MNTRIRNLVKKPWLLVLVVLVAGAMILLRLRTSQIESAAEFHPS
                     PLKVTTEVAARGNLAEKKRYLATMEPLATAEVASQVTARIDEVFVDIGDEVSSGELLA
                     VLDGSEAGYQFQATKSRISEAEAEMEGLRMRKVSIEKNAAYWREEAGRAAFLFSEGAI
                     ARSELEMASLKEAEAQAAFKEIQSRIRALKERQGSLASEAAAFQERLALFSIRSPFDG
                     IVTARRAEPGNLARPGEALFTVEDRSGWRVIFRIPQEDLGLVRIGQTLSGSFGRSSIK
                     GRVDRVSPSVDAARMGRVEATLEKDPGDAAPKAYGRGVSLTIDVTLGTHEDVITIPAE
                     VVIRAGERSFVYTVSGGRLNKQEVVVVASDGERSAVRGLSEGDHVVRNSPFGWAALSE
                     GREVSEE"
     gene            complement(9369..10700)
                     /locus_tag="PLM30_01665"
     CDS             complement(9369..10700)
                     /locus_tag="PLM30_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR53908.1"
                     /translation="MKPRTGSYYLLGATMTAVFLLGSVAALASETEPLTLEKCLDAAL
                     YGNRGIVALRHRVDVSAAKVDETLAKMGPKLFAVGKYLAAGEQQRLWPARYDGEKGVY
                     DDAFTEASVVLEIPLWDGGKTRHRTMADRKLLEAARGSLERQKQKLRYDVERLFFMAL
                     SVKESIGALEASLGSLLQHGEDVQAMVDSGKAARVDTLRMDAEVALTRERLLGARHEL
                     ESLKEDLLFITGIDGKGDDFDLEGPFPETPDIDDLSVEDRASKALEKRPDYQAALDKA
                     AAGKEGVLATLASKSPEFFLRGLHGYRSASNGVGESRTDVELAVEIPLLDGGLSSARR
                     RQAASEAAAAEAEAREMASRVYFEVSEAVRAYEFARERIIVTETAVRAAEEALRIEKL
                     RFRSGKGTTTDVLSSQAAWLSARAGRDAALAEGAIAAARLTFAMGGGSGEY"
     gene            complement(10751..11344)
                     /locus_tag="PLM30_01670"
     CDS             complement(10751..11344)
                     /locus_tag="PLM30_01670"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR53909.1"
                     /translation="MAVRLDTEIRREQIISAALGLIGEKGLRNLRMPDIAQKVGLVPS
                     ALYRHFSGKQELVLAIVDSIRTSLEKHLTRSGEETPDPLERLAFIMDEHLKMIEGSPG
                     MPLLVLSEDAAFGDPKMRRRVLEIHEFFREAVADIMKEAIKTGRVGPDTDPGEAALVF
                     VSLIQHTAVMTAISGGDVDLGPLAGKAWRKYVKDLVG"
     gene            11715..12212
                     /locus_tag="PLM30_01675"
     CDS             11715..12212
                     /locus_tag="PLM30_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR53910.1"
                     /translation="MERGREKDRPVLTRQFEGPGGRLASGAKGKQAGFTLVEVLISIL
                     ILSIALFSMVTLVTHVVALQADLEEKEEASLEAASVLKKMEYLPLKEITEGMSLEKYR
                     NNLKYTPQLFIDGEVTAKGETVGKTLRFKVSWSGVRGEKELEFIRTISATGEQTRGKR
                     MKGEQ"
     gene            12202..>12434
                     /locus_tag="PLM30_01680"
     CDS             12202..>12434
                     /locus_tag="PLM30_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR53911.1"
                     /translation="MNSNGNGRMIPGPLGKTAANERDLSSWEVRVRDLASRVLSCRRV
                     SFSRTGRRGFSLAEVLITILVTAVIMGAVITVLS"
BASE COUNT         2261 a         3992 c         3862 g         2319 t
ORIGIN      
        1 tcggacaagg taaagggaca ataattcacc cgtcggtcaa agtcaagaat gattttgaat
       61 ttggtcggga agggatcggt atcgaacctt cggggcaggg ttatcgaccg gatcctccgt
      121 cctttgccct tttcacggct cccggcgggg acggtactct cgactcgttt tccagcatcg
      181 acctctcgag ttcctcggct gccagctcca ccacggggag gcatccgaac ctgctgtccc
      241 tgtaaaggtc cttcagggga ccgcagtcga tggcgccgtg cctggcggcg aatccttcca
      301 ggaaggccct gcatagctct tcgaccaggg ggttctcatg ggatctcgtt gaggtgaact
      361 tgaccgagat cgcggcgatg gacccggaca aagccccgca gcaggtggag ccgatgccca
      421 tgccgccccc gaagccggcc atgatcctga gccccacggg gtcgagcccc agggagtatt
      481 cgtcattggc cgcgcaaagg atggtctcgg cgcagttcat gtcctcgagg agatagtagt
      541 acctgaccct gtcgatgagc atcggaagac ctcccgggta tggatcagat gtgttacggt
      601 tacccgcccg gccttttcct gagatctatc ttgcaatact cgtgctcctt caggtcgcac
      661 cagagcatgc ccgccagggg ttcacccgcc cccgagagga gccttatggt ctcggccgcc
      721 tccagggagg cgacaaaggc cggcgtgaag ggggggttgc ccgcgatatc ttccacgccc
      781 ttaccctctc ccgtagccag ctcccaggga gccctgtcgc cgggaaggat cacgcaggcc
      841 tgaccccaga aacccccgat ggcaccgtgg accaagggta tacccaactg gcggcaggcc
      901 gaaaggagcg tcgcccgggc ctcgttgctg tcgagggcgt cgacggcgac ctgacagccc
      961 tcgaggatat caagcgcatt gtcggccgtc aggaagaggc tccaggccgt cacctccacc
     1021 gccccgttga tggcttctat cctcttttcg gcctcgaggg cctttgaagc gccctcgttg
     1081 tcctcccggc tcagggactg cctgttgagg ttgctgtcgc cgaagacgtc gccatcgacg
     1141 acgacgatct ccccgacgcc ggccctggcc aggatctcca ctatccagcc cccgaggccc
     1201 ccgcagccta tcaccgcggc ccgggaggcc aggagcctcg cctggccttc caggccgagg
     1261 gtccccaggc tgcgctcgta gcgggtgggg cagatgccct gctcacaggc ccagagctcg
     1321 accttcctgg cggggaggcc ggcctccctc gccgcatccc tgcagatccg ccagggcacc
     1381 aggcggtaag tccctttggg tttgctcttt gagattatcc tttcctgcca ggtggagggc
     1441 atcgttcggt ctcccttccc gtttctcgaa tgacagggca tagaacgcta aataagcata
     1501 ccatcacggg ttccagggag gtgaatcagt gtaaactgta tttgagcatt ctggcctatc
     1561 aaaagcagga ggggggatcc ccttgaaaag gattttcgtt tccggggctt ccggcaacgt
     1621 gggaaaactg gtggtcagga atgtcctcga gagggagggc catgaactgg ccgggggttg
     1681 gtgcctcgag gcgggccgtg acctgggcgt cctggccggc atggcgccgg cgggggtgat
     1741 ttcgagcgcc gacctggcgt cgggcctccg tgactcctcg cccgacgtgg tggtcgattt
     1801 cacctctacg gcggtcctca tgaacaacct ccgggtctat gccgacgccg gcgtggacgc
     1861 cgtcatcggg acgacgggcc tcaccgacgg ggagatggcc aaggtagggg agatggtgaa
     1921 ggagaagggc ctgcgctggt cggtgatctc caactacggc ctggggataa acctggtgat
     1981 ggatttcctg aggaccgcca ggcagtacta tccctatgtc gccgtcaccg acaggcatcc
     2041 cgcgaggatg gccaacgccc ccagcgggac ggcggccacc ctggccggca tcgccgccga
     2101 gggccccaag ggcgaggtag aaagccgcga ggtcttcccg ggcgtgatgg gcgccatgat
     2161 ctcgggtgtc ccggtgacct cccagagatt tccctacccg ggcccctatt cggagcacga
     2221 ggtcaccctg gccaggcagg acgaggttat cagggtcacg gtccaggatt tttccagcga
     2281 gatctacatc gacggcatct tcctggccgt ggggaaggta ggcgaccttc cccgtggaac
     2341 gctggcccgt tcgctgaagg agctcgtcta ggagtttcag agaccgccac tggcggcctg
     2401 gaaggtgatc ttccccccgg ccatggtcag gagtggcttg acgtccttta gttcccccgg
     2461 cggggtgagg tcgatatcgc ggttgaggat caccaggtcg gccctcgaac ccggccgcag
     2521 tgagccgaac ctgtcaccct gcccgacagc ctcggcggcg tttaccgtga agagggcgag
     2581 agcctcgtcc agggtcaacc tctggccggg gagccatcct cccgcaggtc ggccttcgtc
     2641 gtcgaccctg ttcacggcgg cccagacgcc cctgaagggg ttggtgggct ccacgggcgc
     2701 gtcgctcgac ccggtgagta gcagaccggc ccggagcatg ctcttccagg cgtaggccca
     2761 ccccagcctg gcgcggccta tcctgccttc ggcaatggcg atgtcactgg ggacgaaggc
     2821 gggctggaca tcgcagaccg cgccgagccg ggccaggtcc cggacctggt ccggtcggca
     2881 tacctgcagg tgaacgaccc tgtgacgcag ccccgacggg agggggggca ggtcccccag
     2941 ggataccatc gcccggatga actggtcgac ggcggcgtcg ccgatggcgt ggacctgcaa
     3001 ctggttcccg gcggcgtgtt catcccttgc ctgccggagg aactcctcgt cgctcatgtt
     3061 gagagccccc cgggtggagg gggcgtcctc gtaggggaag gtcatcgccg ccgtccggcc
     3121 ccccagggag ccgtcgagga agaatttcct tcccccgtag cagagccact catcgccctg
     3181 gccgctggac tcccacccgg ggaggggctc gtcgctgtag aggcgaaccc tgatggggag
     3241 ttccccgcgc tcgtgtagaa cccggtaggc ccccaggtcc tcccccaggc cgtaggacgg
     3301 agcgctgcag gtatggacgc cggtgatgcc ctccgacgcg agggagagca tggtctcctt
     3361 caaggcccgg gccgccccgg ccgtgtccct ctccatggcc cggatcaccg gtccggccga
     3421 gccctcgagg agcgtcccgg tgaaacgacc ccgctcgtcc cggacgacgc cggcgccggt
     3481 gaagcccgcc gccgagagcc cggcctcatg gagggccctg ctgttgacca cttgcacgtg
     3541 ggtgcagacc cggacgagga ggacggggtt ggggagaccc agcccgtcca gctcctccag
     3601 cgtgggcagg gccgtgtcgg gccagcggga gttgtcaaaa cggaccccgt ggacccagcg
     3661 gttggggccg agctctgcgc tcttacggcg gacccgctcc aggaggtctt ccctcgatga
     3721 cgcctcgtaa agggacaggg ccgatccctg cctgacgagg gccccgaggt gcaggtgcga
     3781 gtccgtgatc ccggggagga tcgtccgacg gccggcgtcg atccattcaa cgccccccgg
     3841 ttggcggccg tccctgacct cctcgtaact ccccacggct atgatgtcgc cgcccttgac
     3901 gacgagcgcc tcggcccggg gcctccgcgg gtcgccggtc cacagggagc ccctgatccc
     3961 gatggctgtc ccggccattc cagggggccc cttctagatg tccaccggga tgaagtgcct
     4021 cgcgaccagg ccgatcagga aggagatcgc cgcgacgccg aggcttatgg ccgccatctc
     4081 caggaaagcg ggcctgaagg gcgtctcctt gacaacggaa acgaaaaagg agaagagcag
     4141 tatgaccact agcgcgttga aaagggacca ggccagggcg gcgtaaacat tcccgacgat
     4201 gaaaaagggc gccaccagca gggcgacggt gaccaggtag gcgatccccg tgtagaccgc
     4261 ggccttcagc gggttgaggt cgccgctgtc ggtcctctgg gagaggaatt ccgaggcgcc
     4321 catcgagagg gtggcggcca cgccggtgat gaggccggcg acgcccacca ccctgccgtc
     4381 ggtgagggcg aaggtgaaac ccgccagggc gccggtcagt tccaccaggg cgtcgtttag
     4441 cccgagcacc atggacccga tgtacttgag cctctcctcg tcgagaaggt cgatgagggc
     4501 cttctcgtgt tcctcctcct cgtgcattat ttccgtcgcc acgggaagga cgtccgccac
     4561 ggcgccgtag gcctccccgg cggagccctc gttccgctcc atgatcttta tggcgaaggt
     4621 gacgccgaac acgaaggcca gcagcaggta ccagtacagc atcagccggt cgggcgcaag
     4681 gctctcaccg gtgtagcgcc gccatatccc ggcgtgccgc ttctcgtcct gggcgatctt
     4741 ttcaaggacc tcccggttct tgcccttcat ccactgggag agcctcttgt aaacgtggta
     4801 ctcggtgatc tccgtcctct ggaagacctt gatcttttcc agtatttccg gtgtgatccc
     4861 ctctttaccc atcggttgct ccctccctcg atctcgcctg ttccaggtcc agccccgcac
     4921 ctcgccgggt cattttgccg aggggcccgg ttccgtataa tatatcagaa agcagccccg
     4981 gggccttgcg tcgcgggaac tcacccgggc gtccctcgac gccggcgtgg cggatatggt
     5041 tggtgcgccc ctcacgcgcc ggggtcttcc cctgttctac cgaccagggc cgccgccctg
     5101 gaactgatat cgtctatgac ggaatagatg accgggacga agatcatcgt gacaaaagtc
     5161 ccgaatatca gcccgctcgc ggcgacgatc cccagggggc tcatcctctc caggccgacg
     5221 gcccactcca tgaccagcgg cgtcaaaccg attatggtcg aggtgacggt catcaggatc
     5281 ggcctcgtgc gggtcttgac cgacctgacg acggccaggg ttcgcgaagc cccccgcttc
     5341 ctggattcaa tgatgaaatc caccaggagg atgctgttgt tcacgacggt cccggcgagg
     5401 aggatgaacc ccatggcggc gttcatcgac atcgatttgt tgaagagaag aagcccccac
     5461 aacccccccg cgacagccgg cgggactatc gaaaggatcg tcaaagggta aaggaaggac
     5521 ctgaaaaggg agaccagcag gaagtagagg agcactaccc ccaaaaggag ggccttgccg
     5581 agcatggcca tgctctctcc aaggtcagcc gagctccccg cgacctcgat ggagtaaccg
     5641 tccgggggct ggatgccccg gagccgaagt ttcacctcgc tcgtcacatg ctttatggtg
     5701 cggccccggt tgaccccggt tatgtctatg gtgttcctga gcctttccct ggtgatgagg
     5761 ggcgcttcag gaacggtgcg caccctcgcc agtgccgaaa ggggcagaag gccgtccccg
     5821 gtcacgatgg tcacatccag gatatcctcc tctttctcca ggtactggtc cccgtaaaaa
     5881 accctcaccg ggatatcgga gaaagagggg actttcaggt aggtggcggt gcggccattc
     5941 agggccgtgc ccacttcacg ggcgatgtcc gtcggcgaag tgcggtaccg cgaggcaagg
     6001 taggggtcga cctcgatatt ccgggtctcc ttctctatga accagccctg gtgtacgtcc
     6061 cccaacccct ttacgccctc catggcggac acagcttccc tggcgaggct atccagcagg
     6121 ggcgcatcgg ggccgctgat gatcacatcg agcggggcct tggttgtggc cctcggcgtg
     6181 gcgccgtact cggtgagcct tgaccatcgc aacccctcga tacttttgaa atcagccctc
     6241 cagcggtcca ggatctccca aatgttttcc ttccgcttcg aacgatccac catgaggacc
     6301 ttgatccgcc cggcctgggg ggttgaaccg cccccgaagc tgggcgaccc gggctccgag
     6361 cccacctgcg atgaaaggga caatacgtgc ttctcctcga ggatcttctt ttccatggcg
     6421 gtcagggtct tttcgacggc ttccggagat cttccagcgg ggctttcaaa ttcgatgaag
     6481 gcaatgcccg tgtccatggg gggcatcatc tcccctccca aaaggggcat caccaggcga
     6541 agggtgagca ggaagaaaag ggcgaaggct gcgaccgtca ggccccggtg cctcaggctg
     6601 actttgagca gttcgatgta aaaagtgacg acctttttca gtaccccgtc gaaccaggag
     6661 gttatcttcc ggagtacgcc tgacgggcgg tgtcctttgc cctccagcac gaacccggcc
     6721 aggaggggga ttatcgtgag cgccgccacg agggaggcgg ccagcgtggc gaagagcacc
     6781 atgtttaggg gcctgagcac ccggctcgtg tagccgctgg aacccatgac ggggagaagg
     6841 acgataacgg tggtcagcat gccggccgtc acggaaaggg ccacttcctt cgccccggcc
     6901 acggccgcgc cgcgtgggtc cttcatgccc gtcgcctcgt ggaccctgaa aatattctct
     6961 aggaccacca ccgaagcgtc gaccaccatc ccgacggcga cgatcattcc cgtgagggtc
     7021 accatgtcga tggtgtaggg gctggcccag ataacggcga ggccgaacag gaatgaaagg
     7081 gcgatgctga ctgaggatac cagggccgcc gcgaggttgc ccaggaacag gaagttcacc
     7141 accactatca ggattatcgc ctgcacgagc gatccgtaca tgccggagat attgagttcg
     7201 atgaggggct tctggctgtc ggtcacctcg atccgcatgc ttctgaagcg ggactccaac
     7261 tccgggatga ggtccgtgac agctttcacg acccgaatgg tatcgcttcc ttcggccctc
     7321 agcacattca ccgcgatggc cggccgcccg ttcccgtgat agaggctcct ttggggcatc
     7381 tcgcctatcc cgacacgcgc tacatccctg aggttcaccg caccgccttc cggtgaggcg
     7441 atcacggtat cccctacttg atcaaccccg ggaaaaagcg agtccagggt gaaatggatc
     7501 tccgcgccct ccacggtcaa gcgtccggcg ggagcggaaa tgttctgctt ggcgatcgcc
     7561 ccgatcaggc cttcgagaga aaggcccgaa gcggcgagga ggtccctgtc gaccgatacc
     7621 tcgacggaag ggtgatggcc tccgaagacc tcgacttcgc ccaccccggg gatccgtagc
     7681 agttcgtccc tgatgacgtg ttccgcaagc aagcggatct cggagagagt cggcccccct
     7741 tcgtcaaggg gggacagggc aagggtcacc gccggggcgg tgcggctggt gatgctgtag
     7801 acaaggggtt ccgccgttcc ggccgggaac ctgggcgtta cggccgcgag ggcgttccgg
     7861 acctccgtca cggcttcgga cacctttttt ccataggtga actccgcccg gagcgaagaa
     7921 acgccgtcgg cggtcgtgct ggtctggttg gccaaaccgt tgaggccgct gatctcccgc
     7981 tcgaggggct ccgttacggt cctggccatc tccggggccg aagccccttc cctgacggta
     8041 acgaccagca cctgcggagg tgtggtgtcg gggaaaaggt ccctggggga tgacatcaca
     8101 ccggccaggc ccaggacacc caccgcgcag gccagggcaa ggacaagata ggggtgctcg
     8161 agggaccact tggcaaggtt cattcttcgc tcacctcgcg gccctcggat aacgccgccc
     8221 acccgaaggg gctgttccgg accacgtggt cgccctcgga gaggccccta acggcggacc
     8281 tttccccgtc ggaggccacc acgaccacct cctgcttgtt cagcctgcct ccggagaccg
     8341 tgtagacaaa agatctctcc ccggcccgta tgaccacttc cgccgggatg gtgatcacat
     8401 cctcgtgggt gccgagggtg acatcgatgg tcagggagac cccccgcccg tacgccttcg
     8461 gcgcggcatc gcccgggtcc ttctcgagcg tcgcctcaac gcgccccatc ctggccgcgt
     8521 cgacggaggg cgagacgcgg tcgacccggc ccttgatgga agaccggccg aaggagcccg
     8581 atagggtctg accgatcctg acgagtccca ggtcttcctg gggaatcctg aagatcaccc
     8641 tccacccgct cctgtcctcc acggtgaaaa gggcctcccc cggcctggcg aggttccccg
     8701 gctccgcccg gcgggcggtc acgatgccgt cgaagggact cctgatggag aagagcgcca
     8761 acctctcctg gaaggcggcc gcctcggaag ccagggaacc ctgcctttcc ttcaacgccc
     8821 gtatcctgct ttggatctcc ttgaatgccg cctgggcctc cgcttctttc agggaagcca
     8881 tctccaactc cgatctcgct atggcgcctt cagaaaacag gaaggccgcc cgcccggcct
     8941 cttcccgcca gtaggctgcg ttcttttcga tggagacctt ccgcatcctc agtccctcca
     9001 tctcggcctc ggcctcggaa atgcgggact tggtggcttg gaactggtac cccgcttcgg
     9061 aaccgtccag gacggcaagg agttccccgg atgagacctc atcgccgatg tcgacgaaga
     9121 cctcgtcgat acgggccgtc acctgtgagg cgacctccgc cgtcgcaagg ggctccatcg
     9181 tcgctaggta ccgcttcttt tccgccaggt tgcccctggc ggcgacctca gtcgtcacct
     9241 tcagaggact ggggtggaat tccgcggcgc tctcgatctg gcttgtccgt aacctgagca
     9301 ggatcatcgc gccggctaca agcacgacaa ggacaaggag ccatggtttt ttgaccaggt
     9361 ttctgatcct agtattcacc ggatccgcct cccatcgcga aggtcagtct tgccgcggcg
     9421 atggcaccct ccgccagtgc ggcatcccgt ccggcgcggg ctgacaacca tgccgcctgg
     9481 gacgaaagca cgtcggtggt cgtccccttg cccgaccgga acctcaactt ttctatccgg
     9541 agggcctctt cagccgccct gaccgccgtc tccgttacaa tgatcctctc ccgggcgaat
     9601 tcgtaggccc gtacagcctc ggagacctcg aagtaaaccc gggaagccat ctcgcgggcc
     9661 tccgcttccg cggccgccgc ttcggaggcc gcctggcgcc tccttgccga agatagtccc
     9721 ccgtcgagaa ggggtatctc cacggccagt tccacgtcag tcctggattc gccgaccccg
     9781 ttggaagcgc tccggtatcc atgcagcccc ctgaggaaaa actcggggct tttcgacgcc
     9841 agggtcgcga gaacgccctc cttgccggcc gccgctttgt cgagggcggc ttggtaatcc
     9901 ggccttttct caagggcttt cgaggcccta tcctcgacgc tgaggtcatc gatgtccggt
     9961 gtttccggga aagggccttc caggtcaaaa tcatcgccct tgccgtcgat cccggttata
    10021 aagaggaggt cctccttgag actttccagt tcatgccttg cccccagcaa ccgttcccgc
    10081 gtcaaagcca cttccgcgtc catcctgagg gtatcgaccc gcgccgcttt gccggagtcg
    10141 accatggcct gcacgtcctc cccatgctgg agcagtgacc ccagcgatgc ttccagcgcc
    10201 ccgatacttt ccttgacgga aagggccatg aaaaagaggc gctccacgtc gtaacgcagt
    10261 ttctgtttct gcctttcgag agaacctctg gcggcctcca gcagtttccg gtccgccatg
    10321 gtcctgtgcc tcgtctttcc cccgtcccag agaggtatct ccaggacgac cgatgcctca
    10381 gtgaaggcgt cgtcgtagac gcccttctcg ccgtcatatc gcgccggcca aagcctttgc
    10441 tgctccccgg cggccaggta tttccccacg gcgaaaagct tcggccccat tttcgccagc
    10501 gtctcgtcga ccttcgcggc ggaaacatcc acccgatgcc tgagagccac gatgccgcgg
    10561 tttccgtaaa gagccgcgtc aaggcatttc tcgagcgtca agggttctgt ctccgaggcc
    10621 agggccgcga cggacccgag caaaaaaacg gcggtcatcg tcgctccaag gaggtaataa
    10681 gatccagtcc tcggtttcat tatttcacct cgcaaaagtg aatgatcatt cactaaaatg
    10741 aaaaataatt tcagcccaca aggtccttga catatttccg ccaggccttt ccggccaggg
    10801 ggccgaggtc gacatcgccg ccgctgatgg ccgtcatgac cgctgtatgc tggatgaggc
    10861 tcacgaaaac aagggcggcc tccccagggt cagtatcggg gccgaccctc ccggtcttta
    10921 tagcttcctt catgatgtcg gccacggctt ccctgaagaa ttcgtggatc tccaggaccc
    10981 ggcgccgcat cttggggtcg ccgaaggcgg cgtcctctga aaggaccagg agaggcatcc
    11041 ccggggatcc ctcgatcatc ttgaggtgtt catccatgat gaatgccagc cgctcaaggg
    11101 gatccggggt ttcctcgccc gaccgggtga ggtgcttctc gagggacgtc cggatgctgt
    11161 cgacgatagc gaggaccagt tcctgcttac cggagaaatg ccggtagagc gcggaaggga
    11221 cgaggcccac tttttgggcg atgtcgggca tcctcaagtt tctgagcccc ttctccccga
    11281 tgaggccgag ggccgcgctg atgatctgct cccttcgtat ttccgtgtcc aacctgaccg
    11341 ccatctacca tcaacctccg aagtgaatga tcattcacat tatagccctg tttttctgct
    11401 ttgcaagggg gggtatttgt tttcaaggtt cggagtggcg tgaattaccg ggggaaggca
    11461 ctggggtctt tccgggtact gatcgagccc cccttgccgg gggaggtcaa aacctcactg
    11521 aggaagccga cgaaagggcg cctccgcagg cgagagggga agaggggagc ctgggtagtt
    11581 tcatggtatc gggctggttc caagtcggaa cgaccggtgt gcttccgccg cctatccctt
    11641 cccatgggta aagtgatata atcaaccaaa gccaccggcg aggcgcgcgg aaaactttca
    11701 agaggaagag agccatggag agaggtaggg agaaagatcg cccggtttta acgcgccaat
    11761 ttgaaggacc tggaggacgc ctcgcttcgg gagcgaaggg aaaacaggct ggcttcaccc
    11821 tagtggaggt cctgatctcg atcctgatcc tttccatcgc cctcttctcg atggtgacgc
    11881 tggtcactca cgtcgtggcc ctccaggccg accttgagga aaaggaggaa gcctcgctcg
    11941 aggcggcctc ggtgttgaag aagatggaat acctccccct taaagaaatc actgaaggca
    12001 tgtcgctgga gaaatacagg aataacctga aatacactcc tcaacttttc atcgacggag
    12061 aagtgaccgc caagggcgag accgtcggga agaccttgag gttcaaagtc tcctggagtg
    12121 gggtcagggg ggaaaaggaa ctggaattca taagaaccat cagtgccacc ggcgaacaga
    12181 ccaggggcaa aaggatgaag ggtgaacagt aacggcaacg gcaggatgat cccgggcccg
    12241 ctgggcaaga cggccgcgaa cgaaagggat ctatcttcct gggaagtgag agtgagagat
    12301 ttggcatcca gggttctttc ttgccggcgt gtttccttct cccgaacggg gcgaaggggt
    12361 ttttcactag cggaagtact gatcaccatc ctggtgacgg cggtcatcat gggcgcggtg
    12421 atcaccgtgc tgtc
//
LOCUS       DAOSRG010000021        12432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5092_length_12432_cov_7.401066, whole genome shotgun sequence.
ACCESSION   DAOSRG010000021 DAOSRG010000000
VERSION     DAOSRG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_12432_cov_7.401066"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLM30_01685"
     CDS             complement(<1..841)
                     /locus_tag="PLM30_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOR53912.1"
                     /translation="MTALGQLRVELSRLKDIDRVLVAAVVPLMVIGVLSIYSALAGKG
                     GVAMDMALKQSAWCALGAAAFAASFAFGHERALKYGYWIYGGVLLSLVAVLVAGYTAK
                     GATSWFDFGAFRFQPSEPAKLSLSVVLAVFLCRYPPKTLVNISGAILLSSLVVVPVLL
                     QPDLGGVVVYAFMIFVALVLAGMPRKYILLLAGVALLSLPLGWSMLKEYQKLRMLVFL
                     NPSIDPLGAGYNVIQSRIAVGAGGSFGKGFLQGTQSRLHFLPEAHTDFVFSVFSEEFG
                     FAGA"
     gene            complement(838..1113)
                     /gene="minE"
                     /locus_tag="PLM30_01690"
     CDS             complement(838..1113)
                     /gene="minE"
                     /locus_tag="PLM30_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOR53913.1"
                     /translation="MGFLDRFFRKETPRQSARERLQLVLIHDRADISPDTMERIRKDL
                     IKVINNYMVINEEGIEMDLERDGRSVALVASIPVIEVKRKHPRKRDK"
     gene            complement(1117..1932)
                     /gene="minD"
                     /locus_tag="PLM30_01695"
     CDS             complement(1117..1932)
                     /gene="minD"
                     /locus_tag="PLM30_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOR53914.1"
                     /translation="METRVIVVTSGKGGVGKTTVTANLSARLAMEGFKVVAMDADIGL
                     RNLDVVMGLENRIVYNMVDVIEGTCKLPQALVRDKRVENLFLLPAAQTRTKDAVTSDE
                     MKGLCDSLRGEYDFVLVDSPAGIESGFRNASAGADEALVVATPEVSSVRDADRIIGLL
                     ESFGKRSINLIVNRVRPDMVRKGKMLGVSDVREILAVDMIGIIPEDETVVVSTNRGEP
                     LALSNTSPASRAFEKIAGRILGRDIPLQDLDDLEEKGLFANFIKLFGRREGRG"
     gene            complement(1980..2660)
                     /locus_tag="PLM30_01700"
     CDS             complement(1980..2660)
                     /locus_tag="PLM30_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOR53915.1"
                     /translation="MTARNVKDILELKGEGEHLRLIVPRGLKGRGTRAQAGAFLEKAA
                     AILKDAPVVVEIKGEGWGRDDIFSIVETVLLPAGASVLSWSPEGEDVRSWMKREGFAL
                     FSPPPETEAPAPVRPVAESLVVDESLRSGRKVEHDGDVMVLGNVNEGAEVFAGRSVLV
                     LGRLRGLAHAGLTAPEEAFIVSGHFEANQVRIGDRISYMTEDCPWWNKTVSMRILDGS
                     IVVREIVP"
     gene            complement(2688..4415)
                     /gene="mrdA"
                     /locus_tag="PLM30_01705"
     CDS             complement(2688..4415)
                     /gene="mrdA"
                     /locus_tag="PLM30_01705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR53916.1"
                     /translation="MPGIRRSLLTRLSIWRILVMLSLVLLLAVLFRLQVLQADLYVNL
                     AARNRLRLVRMAPARGRVLDANGTVLATNVQTFDLMVYPLDLRDRETAAEVSRFLKAR
                     GIPLDEGLMLARVTKEYTVPYRAVTLLPNLTLAQLSSLVSAPDFPGQLIPVPVWRRVY
                     PAGPLVAHVMGYVGEVTREELQRYAPGDYRGGDFVGKGGIEEYYEGLLRGTPGEEALE
                     VDARGRKVKRVAYLEPQRGEDLQLALDLGAQRLASELMAGRAGAVFAMDVHNGDVKVL
                     FSSPGFDANPLAWGISGREWDELVRDPLRPMLNRVITGTYPPASTFKVVPAFAALAEN
                     AVTRSTTFTCTGGMRVGNRLFRCWKREGGHGPVNVVTGLRDSCDVFFYQAGLRVGIDR
                     LVKWASAFGVGEPTGIDLPGEARGNVAGPAWKLERFREKWYPGDTANYSIGQGFLLLT
                     PIQLARIYAAIANGGYLVTPRLLEGAEERVSARLPEKPLPIVRQGVEEAVLRGTGRRA
                     GEYGVSVAGKTGTAQNPHGDDHAWFVGYAPVENPRYVAVALLEAGLHGSSAAGPVVGE
                     LLGYLCKFD"
     gene            complement(4402..4869)
                     /locus_tag="PLM30_01710"
     CDS             complement(4402..4869)
                     /locus_tag="PLM30_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53917.1"
                     /translation="MMPLIGLWYLQDLFQALLSERFLAPDLFLVFLIYRVTKNPREAS
                     PVIWPALVGGLFWDLRWTSLPGFTGAVYAFLAALCVVAWNHIPYPGRNAGLFFLLAFS
                     TQFFASLVRFIFWGGFRGSLTGALVFQLCSAVPVIIAAALAVAAGMDEDNARH"
     gene            complement(4871..5644)
                     /gene="mreC"
                     /locus_tag="PLM30_01715"
     CDS             complement(4871..5644)
                     /gene="mreC"
                     /locus_tag="PLM30_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOR53918.1"
                     /translation="MLKGVSSAWLHGLAATFIGLLFLAVSFGSPVTYKVSELAAVILE
                     YPERPALWARRLVSEGVSWAGGQRKLQERVRDLEFENARLRATLQERPDAVVSPAPGL
                     VHAWIDARPPSFWWTEVRVGRGSNDGVKVGMPALWKGFLAGRVSRVFAGHSWVDLLTS
                     TGLMVPVVVEETRDLGVLTGDGRGGIQLLYIPEEKGLRSGMRLSTAPVSDILPAGLPV
                     GEISDAPPTIEDGFRVYRVLPGVDFSVMDSLLVMTGGKP"
     gene            complement(5708..6763)
                     /locus_tag="PLM30_01720"
     CDS             complement(5708..6763)
                     /locus_tag="PLM30_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOR53919.1"
                     /translation="MFGPFSGLFGKDIGVDLGTTNIVICVKGEGVTMNEPSVIAFRKA
                     SRKSNKEIIAYGSEAKAMAGKTPVGVETVQPVQSGVIGDFEMTGALIRHFLSLANNGI
                     RPMGHPRVIISTPVDITEVERKAFIDATLDGGAREVYVVEEPVAAAIGVGLPIDEPRG
                     NMIVDVGGGTSEVAVLSLGGVVVSNSLRVAGDEMDAALINMMRQNYTLSIGSATAGEL
                     KNAIGSALPMNSEKEFEVRGRNLKDGLPKGIRISNTEVREALNPVLTRIIEMVRETLE
                     RTPPELARDIADQGLVLSGGAALLEGLGKRISQDLKVPVLTAERPLLAVADGIAKLLD
                     RFDYMKKILVSVGHGVR"
     gene            complement(6771..7475)
                     /locus_tag="PLM30_01725"
     CDS             complement(6771..7475)
                     /locus_tag="PLM30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOR53920.1"
                     /translation="MKTAASTRAELPRERVERLGPQALSLVEIIAILLRTGARGKNVL
                     ELASSLVKEYGDLQGLSRASFREFMEMGGLGKAKSATLVAALELARRLYAEEKASAEG
                     EGREHFRSCLARWSARFSDEPREFIAAAFVDRGGKVMEEDIISWGGLEGAVLDLKYLL
                     RRAVRLDAAAVLLLHNHPDKSLDPSVEDRLLTEHVQRKLEALGMDFLGHYVTAGGQLS
                     EVQHQGPGCSRYSESW"
     gene            complement(7472..8113)
                     /locus_tag="PLM30_01730"
     CDS             complement(7472..8113)
                     /locus_tag="PLM30_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR53921.1"
                     /translation="MFLERLPLGALWTNGYLVWDDQGTGFFIDPGGDPQDILSLVDKR
                     GVDLEFILLTHGHADHIGGLGAIRSRARGGVLVHEDDAPMLSRPESNLSAFVGGNIEL
                     APAEKLLKDGDVVSAGCLLVRVIHTPGHTRGSACFLVGEESEDPLLFSGDTLFAGSVG
                     RSDLPGGDEGTLMASLEKLTCLPDTTRVLPGHGPETTIGQERRHNPFWPGGRR"
     gene            complement(8116..8562)
                     /gene="dtd"
                     /locus_tag="PLM30_01735"
     CDS             complement(8116..8562)
                     /gene="dtd"
                     /locus_tag="PLM30_01735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR53922.1"
                     /translation="MRAVVQRVTGARVLVDGRVAGSIGKGLCVFLGVAPEDGAEEVEW
                     MSGKITNLRVFEDSDGKMNLSLLEEKGAMLVVSQFTLYGDCRKGRRPSFASACEPEKA
                     KRVYEDFIRSVSSRGISVESGVFQAYMQVEICNDGPVTLIIDTVGG"
     gene            complement(8570..10738)
                     /locus_tag="PLM30_01740"
     CDS             complement(8570..10738)
                     /locus_tag="PLM30_01740"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOR53923.1"
                     /translation="MSVKMAWHEFWGKASRYLAREDLVRCGEAVVFASEAHGGQTRLT
                     GDPYIIHSIGVASVLADMELDTDTLVAAMLHDVLEDTQVEPDAIREKFGEPVLTLVDG
                     VTKLGKLPFKTFEDYQAENLRKMFLVMAKDIRVVLIKLADRLHNLRTIQVLRRDKQLR
                     IARETLEIYAPLAHRLGIDQMKRDLEDLSFKITDPDAFFDIKARVRKKLPEREEIINQ
                     AIAILQKRFDEEGIPTLITGRAKHFYSIYEKMRRKNISLEELFDLLALRITVKDVAEC
                     YTALGIIHTIWKPLPGQFDDYIANPKSNLYQSLHTTVLGPSGEPLEIQIRTSDMHWIA
                     EYGVAAHWRYKEKRHKIDKLDERLSWIRQALESQGEGGEASEFLEHIRDDVLSTDVFV
                     FTPDGDVISLPGDSTPIDFAYAIHTEIGHKFVGAMVNNRIVPIDYPIQNGDIIKILTS
                     PQGRPSRDWMKIARSGKARSKIRSWFRQVDKVEKQEKQDKGRELLLRELKRREGEKEE
                     VTRLSGPALRKVASDLGYGHGDDLLAAIGGGSQSASSVASKYLEIEASRTQAGPEIEE
                     VQEEVAPPKRELDTEIVVEGSRGVMVSLASCCKPIPGDSIVGYVTKSRGITIHRSDCP
                     NLADHREDRKVEVYWGELRSARYPARLKIEGADRPGLFAEVAQAIAGLDSSITQVRAF
                     VAGTDRGRMVIDLQVKDLEHLYKIIAKLNSVRGVMEVLRG"
     gene            complement(10870..>12432)
                     /locus_tag="PLM30_01745"
     CDS             complement(10870..>12432)
                     /locus_tag="PLM30_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOR53924.1"
                     /translation="FSGDDPREARAQLDPGLDDSMAGIDLGPFSAPAADLWKSLRGIE
                     RVVVYGDYDADGACATALAMELALASFRQVRYFIPQRHREGYGVHEKIVRAIARNGCD
                     LLITVDCGTRDNDALGVAREAGIPVIVFDHHLPGETLPEGAFVVNPHAGGSGEAQTLC
                     ATGVLWAWARREGIAPPEWVDPRMELVAIATLADHVPLGMLNRAIYSRGVGTLRRSER
                     RGLRALIAGTGHRPEEVDEELLTMKVIPCLNAAGRLGVADIAVDTLLGVPGLEGKVRE
                     LVDLNRKRQSLATEILGEILPKVEIGAPVVMGDYAWPVGVLSGVASRICSEAGVSVAL
                     ASLSGDHVRGTLRVPQGVDALKVLEPLAPRLLSWGGHRSAAGFSVSLSEWSGVRDLIE
                     ENLLSSPPSPAAVQAISYPPGMLTPDEVRSMNSLGPFGAGNPPPLFYAPSRGTVEIMP
                     LGRDGKHVRVRAAGGEFLAFGGAARSDLIRSSLGWTFRPRMNNWKGQDRVDFIMDSIT
                     VSDRSGREGIDP"
BASE COUNT         2239 a         4175 c         3693 g         2325 t
ORIGIN      
        1 aggcgccggc gaaaccgaac tcctcggaga agacgctgaa gacgaagtcc gtgtgggcct
       61 cgggcaggaa atggagccgg ctctgcgtcc cctggaggaa gcccttcccg aaagatccgc
      121 cggccccgac ggctatcctg gactggatga cgttgtagcc cgcccccagg gggtcgatgg
      181 acgggttgag gaagaccagc atcctcagct tctggtactc cttgagcatc gaccacccca
      241 gcgggagaga aagaagcgcg acccccgcca gcagcagaat atacttgcgg ggcatgccgg
      301 ccaggacaag ggccacgaag atcatgaagg cgtagaccac cacccccccc aggtcgggct
      361 gcagcagcac cggcacgacg acgagggatg agagcagtat ggccccggag atgttgacaa
      421 gggtcttcgg ggggtagcgg cacaggaaaa cagccaacac gaccgagagg gacagtttcg
      481 ccggttccga gggctggaac cggaaggccc cgaagtcgaa ccagctcgtc gcccccttcg
      541 cggtgtagcc cgccacgagg accgccacaa gggacaaaag caccccaccg tagatccagt
      601 acccgtactt gagcgcccgt tcgtgcccaa aagcgaagga cgcagcgaag gcggccgccc
      661 caagggcgca ccacgccgat tgcttgaggg ccatgtccat cgccaccccc cctttcccgg
      721 cgagggcgct gtagatggac aggacgccga tgaccatgag ggggacgacc gcggcgacga
      781 ggaccctgtc gatatccttc agcctggaca gttctacccg cagttgccct agggccgtca
      841 tttatctctc ttcctgggat gcttcctttt gacctctatc accggtatgc tcgccacgag
      901 ggctacggac ctcccgtccc tctcgaggtc catctcgatg ccctcttcgt tgatgaccat
      961 gtaattgttt atgaccttta tcaggtcttt ccttatccgc tccatggtgt ccggtgagat
     1021 atcggccctg tcgtggatca ggaccagctg caacctctca cgggccgact gcctgggcgt
     1081 ctccttgcgg aagaacctgt caaggaatcc catcacctag cccctccctt cccggcgtcc
     1141 aaaaagcttg atgaagttcg cgaataggcc tttctcctcc aggtcgtcca gatcctggag
     1201 ggggatgtcc ctccccagta tcctgccggc gatcttctcg aaggccctcg aggccgggga
     1261 ggtgttggac agggccaggg gttcacccct gttggtggac acgacgacgg tctcatcctc
     1321 cggtatgatc ccgatcatgt ccacagcgag gatctccctc acgtcggaga cgcccagcat
     1381 cttccccttg cggaccatat cgggcctgac ccggttcacg atgaggttga tggacctttt
     1441 gccgaaggac tccagcagcc cgatgatcct gtcggcatcc ctcaccgagg agacctcggg
     1501 cgtcgccacc acaagggctt catcggcacc ggcggaggcg tttcgaaaac ccgattctat
     1561 ccccgccggg ctgtcgacca ggacaaagtc gtactcgccc ctcagggagt cgcagaggcc
     1621 cttcatctcg tcggaggtca cggcatcctt ggtcctagtc tgcgcggcag ggaggagaaa
     1681 gaggttctcg acacgcttgt cccgaacgag ggcctggggc agtttgcacg tcccctcgat
     1741 gacatcgacc atgttgtaca ctatcctgtt ctccaggccc attacgacgt caaggttacg
     1801 gaggccgata tcggcatcca tcgcgacgac tttgaaccct tccatggcga gtcgggcgga
     1861 gaggttggcc gtgacggtgg tttttcccac cccccccttc cccgaagtta ctacgatgac
     1921 gcgggtttcc atgcgcgatt cctccgtttc ggccgatggg ccgtcttttt tcatgctcat
     1981 cacgggacta tctccctgac cacgatcgaa ccatcgagga tcctcatgct gaccgtcttg
     2041 ttccaccagg ggcagtcttc ggtcatgtaa ctgatccggt cgccgatcct aacctggttc
     2101 gcctcgaagt gcccactcac tataaaggcc tcttcaggag cggtcagtcc agcgtgggcc
     2161 agcccgcgga ggcggcccag gacgaggacg gacctgccgg cgaagacctc ggccccttcg
     2221 ttgacattgc cgaggaccat gacatcgccg tcgtgttcca ctttcctgcc ggacctcagg
     2281 gattcatcca ccacgaggct ttccgcgacg ggccgcactg gcgcgggtgc ttccgtttcg
     2341 ggaggggggg aaaagagagc gaaaccctcc cttttcatcc aggacctgac gtcttccccc
     2401 tcaggggacc aagacaggac cgacgccccc gccggcaaca ggacggtctc cacgatggag
     2461 aagatatcat ccctgcccca accctccccc ttgatctcga cgacgacggg ggcatccttg
     2521 aggatagcgg cggccttttc gagaaaggcc cccgcctggg cccgggtgcc ccgccccttc
     2581 aaaccccggg ggacgataag cctgagatgt tctccctccc ccttcagttc cagtatatcc
     2641 ttgacgttcc tggcggtcat cgtttccctc cgtcgcgcat ttttttatca gtcaaattta
     2701 cagaggtacc ccaggagttc ccccacgacg ggacccgccg cggagctgcc gtgaagcccg
     2761 gcctcgagga gggctacagc cacgtacctc gggttctcca ccggcgcgta acccacgaac
     2821 caggcgtggt cgtcgccgtg ggggttctgg gcggtgccgg tcttccccgc cacggaaaca
     2881 ccgtactcgc cggcccggcg tcccgtaccc ctgaggaccg cctcttcgac gccctgccgg
     2941 acgatcggca ggggtttttc cgggagcctc gccgacaccc tctcctcggc cccctccagg
     3001 agcctcggag ttaccaggta cccgccgttg gcaatggcgg cgtagatcct ggccaactgg
     3061 atcggggtca ggagaagaaa cccctggcct atggagtagt tggccgtgtc gccgggatac
     3121 catttttccc ggaagcgttc cagcttccag gccggtcccg ccacgttgcc tcgcgcctca
     3181 ccgggaagat ctatgcccgt cggttccccg accccgaagg cggaggccca cttcacaagt
     3241 ctgtcgatgc ccaccctgag gcccgcctgg taaaagaaaa catcacagga atcgcggagc
     3301 cccgtcacga cgttgacggg accgtgtccc ccttccctct tccagcaccg gaagagcctg
     3361 ttccccaccc tcatgccgcc ggtgcaggtg aaggtggtgc tcctggtcac ggcattctcg
     3421 gccagcgccg cgaaggccgg cacgaccttg aaggtcgaag ctggcgggta agtgccggtg
     3481 atcaccctgt tgagcatggg ccggagggga tccctgacca gttcgtccca ctccctgcct
     3541 gagatgcccc aggccagggg attggcgtcg aacccgggcg atgaaaagag gaccttcacg
     3601 tcgccgttgt ggacatccat ggcgaacacc gcccccgccc ttcccgccat cagttccgag
     3661 gcgagcctct gggccccgag gtccagggcc agctggaggt cttccccccg ttgtggctcg
     3721 aggtaggcga ccctctttac cttcctgccc ctggcatcga cctccagggc ttcctcgccg
     3781 ggcgtacccc tgaggagtcc ctcgtagtac tcctcgatgc cccccttgcc gacgaaatcc
     3841 cccccccggt agtcaccagg ggcatagcgc tgcaattcct cccgggtcac ctcgccgacg
     3901 taccccatta cgtgggctac cagggggccg gcggggtaga ccctcctcca gacaggaacc
     3961 ggtatcagtt ggcccgggaa gtcaggggcg ctcaccaggg aggagagctg cgccagggtc
     4021 aggttcggca ggagggtcac cgccctgtag ggaacggtgt actcctttgt caccctcgcc
     4081 agcatcagac cctcgtccag ggggatgccc ctggccttta ggaaacggct cacctcggcg
     4141 gcggtctcac ggtcccgcag gtccagcggg tagaccatga gatcaaaggt ctggacatta
     4201 gtggccagga ctgtcccgtt ggcgtcgagc accctccccc gggccggggc catcctcacc
     4261 agtcgcagcc tgttcctggc tgccaggttg acgtaaaggt cggcctggag gacctggagc
     4321 ctgaaaagga ccgccagcag gagcaccagg gagagcatca ccaggatcct ccagatgctg
     4381 agcctcgtca ggagagacct cctaatgcct ggcattatcc tcatccatcc ccgccgctac
     4441 cgcgagggcc gccgctatta tcacgggtac ggcggaacac aactggaaga ccagggcccc
     4501 ggtgagggag ccccggaacc ctccccagaa gatgaacctc accaggctcg caaaaaactg
     4561 cgtcgaaaag gcgaggagga aaaaaagacc ggcgttcctc cccgggtaag ggatgtggtt
     4621 ccaggccacg acacagaggg cggcgagaaa agcgtagacg gcccccgtaa agccggggag
     4681 ggaggtccac cggaggtccc agaacaaacc acccacaagg gccggccata tcacggggga
     4741 tgcttccctg gggtttttcg tcaccctgta gatcagaaag accaggaaaa ggtcgggcgc
     4801 gaggaacctc tccgagagga gcgcctggaa aaggtcctgg agataccaaa ggccgatgag
     4861 gggcatcatg tcagggtttc cccccggtca tgacgaggag ggaatccatg accgagaaat
     4921 caacgccggg caagacccgg tagaccctga aaccgtcctc gatcgtggga ggggcgtcgg
     4981 agatctcccc cacgggcaac cccgctggga ggatatcgct caccggggcc gtgctcaacc
     5041 gcatcccgga gcggaggccc ttctcctcgg gtatataaag gagctggata ccgcccctgc
     5101 cgtccccggt cagaaccccg aggtccctgg tctcctcgac caccaccggg accattagcc
     5161 ccgtcgatgt cagaaggtcc acccaagagt ggccggcgaa cacccgcgag accctgcccg
     5221 ccaggaaacc cttccaaagg gccggcattc ccaccttgac cccgtcattg gaacccctgc
     5281 cgaccctgac ctcagtccac cagaatgacg gcggcctggc gtcgatccag gcatggacaa
     5341 ggcccggggc gggcgagacc accgcgtcgg gcctctcctg gagggtggcc ctcaaccgtg
     5401 cgttctcgaa ctccaggtcc ctgaccctct cctgcagctt cctctgtccg ccggcccatg
     5461 aaacgccctc cgagaccagc cggcgggccc agagggcggg ccgttccggg tactccagga
     5521 tgacggccgc cagttccgac accttgtatg tcaccggtga cccgaatgaa acggcaagga
     5581 aaagcaaccc tatgaaagta gccgccagac cgtgaagcca cgcggaagat actcccttga
     5641 gcaattcgac accccggttt tcatagatct gcgctctttt cccctgtcac cggcccgagt
     5701 ctcgatgtca ccgtaccccg tgacccaccg aaacgaggat ctttttcatg taatcgaacc
     5761 ggtcgagcaa cttcgctatc ccgtcggcca cggcgaggag gggcctttcg gcagtcagga
     5821 ccggcacttt cagatcctgg gagatcctct tccccaaccc ttcgaggagg gctgcgccgc
     5881 cggaaaggac aagcccctgg tccgcaatat ccctcgccag ttccggggga gtccgttcca
     5941 gtgtctcccg taccatctcg atgatcctgg tcaggacggg gttcagcgcc tccctgactt
     6001 cggtattgga gatcctgatg cccttcggga gcccgtcctt gagattcctg cccctcacct
     6061 cgaactcctt ctcggagttc atgggcaggg cggatcctat ggcgttcttc agctctccgg
     6121 cggtggcgga tccaatggat agggtgtagt tctgcctcat catgtttatc agggcggcat
     6181 ccatctcgtc gccggccacc ctcagggagt tgctcaccac cacgccgccc agggagagga
     6241 ccgccacctc gctcgtgccg ccgccgacat cgacgatcat gttccccctg ggctcgtcga
     6301 tgggcaaccc caccccgatg gctgcggcga cgggctcttc gaccacgtag acttccctgg
     6361 cgcccccgtc gagggtggcg tcaatgaagg ccttgcgctc cacctcagtg atatcgacgg
     6421 gggtgctgat gatgaccctg gggtgcccca tgggacggat accgttgttt gccagcgaca
     6481 gaaagtgcct gatcagcgcg cccgtcattt cgaagtcccc gataacgccg ctctggacag
     6541 gctgtacggt ctctacgccg acgggggtct ttccggccat ggccttggct tcggagccgt
     6601 aggcgatgat ctccttgttg cttttcctgc tggccttgcg gaaggcgatc accgaaggct
     6661 cgttcatcgt cacaccctcg cccttgacgc agatcacgat gttggtcgtt ccaagatcga
     6721 cgcctatatc cttgccgaat aggccggaga agggaccgaa caccactacc tcaccaactt
     6781 tcagaatatc ggctgcagcc ggggccctgg tgctggactt cggagagctg ccccccggca
     6841 gtgacataat gccccaggaa gtccatcccg agggcttcca gtttcctttg tacgtgttcc
     6901 gtaagcaacc tgtcttccac gctggggtca agactcttgt caggatgatt atgaagcaaa
     6961 aggaccgccg ccgcatcaag gcggacagcc ctcctcagga ggtacttcag gtccaggaca
     7021 gccccttcca gccctcccca ggagatgatg tcctcctcca tgaccttccc tccccggtcg
     7081 acgaaggccg cggcgatgaa ctccctcggt tcatcggaga acctggcgct ccagcgtgcc
     7141 aggcaagagc ggaaatgttc ccgcccttcg ccctcggccg aggccttctc ctcggcgtag
     7201 agcctcctgg cgagttccag cgcggcgacc agggtggcgg atttcgcctt accgagccct
     7261 cccatttcca tgaactccct gaaggaggcc ctggagagcc cctggaggtc gccatactcc
     7321 ttgaccaggg atgaggccaa ttccaggacg ttcttacccc tggcgccggt cctgaggagg
     7381 atcgctatga tctccaccag ggagagcgcc tgtggcccca gccgctcgac cctctccctg
     7441 ggcaactcgg cacgggttga ggcggccgtc ttcatcgtcg gccccccggc cagaaggggt
     7501 tgtgcctgcg ctcttgcccg atggtcgtct ccggcccatg gccggggagg accctggtgg
     7561 tgtcgggcag gcaggtgagt ttttccaggg aggccataag ggtcccctcg tccccgccgg
     7621 ggaggtccga cctgccaacg ctcccagcga agagggtatc cccggaaaac agcagggggt
     7681 cctcactctc ttcccccacc aggaagcagg cgctgcccct ggtatgcccc ggggtgtgga
     7741 tgaccctcac caggaggcac ccggcactga ccacatcgcc atccttgagc aacttctccg
     7801 cgggggccag ttcgatattg ccgcctacga aggccgaaag gtttgactcc ggtcgggaaa
     7861 gcatgggggc atcgtcctca tggaccagga ccccacccct cgccctcgac ctgatggccc
     7921 caagaccgcc gatatggtcg gcgtgaccat gggtcaggag gatgaactcc aggtccacgc
     7981 cccttttatc gaccagggaa aggatatcct gcgggtctcc gccggggtca atgaaaaatc
     8041 ccgtcccctg atcatcccac acaaggtaac cgttggtcca tagggccccc aggggcaatc
     8101 tctccaggaa catcatcaac cccccacggt atcgatgatc agcgtcaccg gcccgtcgtt
     8161 gcagatctca acctgcatgt aagcctggaa gaccccgctt tcgacgctga tccccctgga
     8221 tgagacggac cttatgaaat cctcgtagac ccttttggcc ttttcgggct cgcaggccga
     8281 agcgaaggaa ggtcgcctcc ccttgcggca gtcgccgtaa agggtgaact gggagaccac
     8341 cagcatggcc cccttttcct cgaggaggga caggttcatc ttgccgtcgg aatcctcgaa
     8401 taccctcagg ttggtgatct tgccggacat ccactccact tcctcggccc cgtcttcggg
     8461 ggccacgccg aggaagacgc aaagaccctt cccgatcgag ccggccaccc tcccgtcgac
     8521 gaggaccctt gcgccggtca ccctctgcac taccgcccgc acggcccctt caccccctga
     8581 gcacttccat gacgcccctg acggaattca gcttggctat gatcttgtag aggtgttcca
     8641 ggtccttgac ctggaggtcg atgaccatcc tacccctgtc ggtcccggcg acgaaagccc
     8701 gaacctgcgt gatggagctg tccagccccg ctatggcctg ggcgacctcg gcgaagagac
     8761 ccggccggtc ggcaccctcg atcttcagcc tcgccgggta acgggctgaa cgcagttcgc
     8821 cccagtatac ttcaactttg cggtcctcgc gatgatcggc gaggttgggg cagtcactcc
     8881 ggtggatcgt tatccccctg ctcttcgtga catacccgac gatgctgtct cccggtatgg
     8941 gcttgcagca gctggctagg gagaccatga cgccgcggct tccctccacg acgatctcgg
     9001 tgtccagttc acgtttgggc ggggcgacct cctcctggac ctcctcgatc tcagggccgg
     9061 cctgcgtccg ggaggcctcg atctccaggt acttcgaggc caccgaggat gcgctctggc
     9121 tccccccgcc tatggccgcg agaaggtcgt cgccgtggcc gtaccccagg tcggaggcca
     9181 ccttcctgag ggcggggccc gagaggcggg tgacctcttc cttctcaccc tcccgccgct
     9241 tcaactcccg gaggagaagt tcccttccct tatcctgttt ttcctgcttc tccaccttgt
     9301 cgacctggcg gaaccaggac cttatcttgc tccgtgcctt accgctccgg gcgatcttca
     9361 tccagtcccg ggaagggcgg ccctggggcg aggtcaggat ctttatgatg tcgccgttct
     9421 ggatcgggta gtctatgggg acgatcctgt tgttcaccat ggccccgacg aacttgtgac
     9481 ctatctcggt gtgtatggcg taggcgaaat cgatgggtgt ggaatcgccg gggagggaga
     9541 tcacgtcgcc gtcgggggtg aagacgaaga catcggtgga taggacgtca tccctgatgt
     9601 gctccaggaa ctcggaggct tcgccccctt cgccctggga ctcgagggcc tgccttatcc
     9661 aggagagcct ctcgtcgagc ttgtcgatct tgtgcctctt ctccttgtac ctccagtggg
     9721 ccgcgacgcc gtactcggct atccagtgca tatcgctggt ccggatctgg atctccaggg
     9781 gctccccgct ggggccgagg accgtcgtgt ggagggactg atagaggttg ctcttggggt
     9841 tggctatgta gtcatcgaac tggcccggca ggggcttcca gatggtatgg atgatgccca
     9901 gggcggtgta gcattcggcg acgtccttca cggtgatcct gagggccaga agatcgaaga
     9961 gctcctcgag ggagatattt ttcctgcgca tcttctcgta gatgctgtaa aagtgtttcg
    10021 ccctgccggt gatcagggtg gggatcccct cttcgtcgaa gcgcttctgg aggatagcga
    10081 tggcctggtt gatgatctcc tccctctcgg ggagcttttt cctgaccctc gccttgatat
    10141 cgaagaaggc gtcgggatca gtgatcttga aggagaggtc ctccaggtcc cttttcatct
    10201 ggtcgatccc cagccggtga gccagcgggg cgtagatctc cagggtctcc ctggcgatcc
    10261 tcagctgctt gtccctcctg aggacctgga tggtcctcag gttgtgaagc ctgtcggcca
    10321 gcttgatcag gaccaccctt atgtccttgg ccatgaccag gaacatcttc ctcaggttct
    10381 ccgcctggta gtcctcgaag gtcttgaagg ggagtttccc cagcttggtc accccgtcga
    10441 cgagggtaag gacgggctca ccgaactttt cccttatcgc gtcgggctca acctgggtat
    10501 cctccagcac atcatgcagc atggccgcca ccagggtgtc cgtgtccagt tccatgtcgg
    10561 ccaggacgga ggcgacgcct atggagtgaa tgatgtaggg gtcaccggtc agcctggtct
    10621 gccccccgtg tgcctcggag gcgaagacca ccgcttcacc gcatcggacc aggtcctccc
    10681 gcgcaaggta ccgcgaggcc ttgccccaga actcgtgcca ggccatcttg acggacatca
    10741 ccctcgatac ttcggggaaa cggccgacga aactgtccct caggaggcgg agggccttcg
    10801 tctcggccgg gtcgagctta gagggcctct ccagcccggc cgcttcactt atcgctttct
    10861 tcttgtcctt caaggatcga tcccttcccg gcctgaacga tcggatactg ttatggagtc
    10921 catgatgaag tccaccctgt cctgaccctt ccagttgttc atcctgggcc tgaaggtcca
    10981 acccagggag ctccttatca ggtcggagcg agcggcacct ccgaaggcga ggaattctcc
    11041 tcccgccgct cggactctca cgtgcttccc gtccctcccg aggggcatga tctccacggt
    11101 ccccctggaa ggggcgtaaa aaagcggggg agggttcccc gcgccgaagg gccccaggga
    11161 attcatggac ctcacctcgt caggggtgag catcccgggg ggataagaaa tggcctgaac
    11221 cgccgccggc gatgggggag aggaaagaag gttctcctcg atcaggtccc tcacgccgga
    11281 ccactccgat agggatacgg agaaccccgc cgccgaacga tgcccccccc aggagagaag
    11341 gcggggcgcc agcggctcca ggaccttcag cgcgtccacc ccctggggaa ccctgagcgt
    11401 ccccctgaca tgatcacccg aaagcgaagc gagggccacc gacacgcccg cctcggagca
    11461 gatacggctg gccaccccgc tgaggacccc cacgggccag gcgtagtccc ccatgacgac
    11521 gggggctcct atttcaacct tcgggaggat ctcccccagg atctccgtgg cgagggactg
    11581 cctcttcctg ttgaggtcta ccagctccct gaccttgccc tccaggcccg gcacaccgag
    11641 cagggtatcg acggcgatat cggccacccc cagcctgccc gccgcgttca ggcaggggat
    11701 gactttcatg gtgaggagtt cctcatcgac ctcttcgggg cggtgtcccg tcccggctat
    11761 gagggcgcgg aggcccctcc tttcggaccg ccgcagagtc ccgacccccc tggagtagat
    11821 cgcgcggttc aacatcccca ggggtacgtg gtccgccagg gtggcaatgg cgacgagttc
    11881 catcctgggg tcgacccact ccgggggggc gatcccctcg cgcctcgccc aggcccaaag
    11941 aacacccgtg gcgcagaggg tctgggcctc accggagccc ccggcgtgag ggttgaccac
    12001 gaaggcgccc tcgggcagcg tctctccggg aagatggtgg tcgaagacga tcacggggat
    12061 gcccgcctcc cgggcgacgc cgagggcgtc gttatccctg gtcccgcagt cgacggtgat
    12121 cagaaggtcg cagccgtttc gtgctatcgc cctgacgatc ttctcatgga ccccgtaacc
    12181 ctcgcggtgc ctttggggga taaaatacct gacctggcgg aacgaggcaa gggccaactc
    12241 catggcgaga gccgtggcgc atgctccgtc ggcgtcgtaa tcaccataga cgacgaccct
    12301 ttcgatcccc cggaggctct tccagaggtc cgcggccggg gctgagaaag gccccaggtc
    12361 gatgccggcc atcgaatcgt cgaggcccgg gtccagctgc gcccgggcct cgcgggggtc
    12421 atcccccgag aa
//
LOCUS       DAOSRG010000022        12426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5094_length_12426_cov_9.209926, whole genome shotgun sequence.
ACCESSION   DAOSRG010000022 DAOSRG010000000
VERSION     DAOSRG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_12426_cov_9.209926"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..642)
                     /locus_tag="PLM30_01750"
     CDS             complement(172..642)
                     /locus_tag="PLM30_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOR53925.1"
                     /translation="MKSYALAIAMAMLVAAVYAFQNTGEVLVRFLIWELSFPQGIWEV
                     MLFAAGGVLMWLVSLFASMESRSKYVRQVKEKEARIKDLEGEKSTLVEALRFSGERHE
                     AELRVQRSEEGVEGPKVEALGHSVERREGEQEARLPGVTSEEGAAEPEAEKEGQ"
     gene            complement(688..2133)
                     /locus_tag="PLM30_01755"
     CDS             complement(688..2133)
                     /locus_tag="PLM30_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR53926.1"
                     /translation="MSAASFDRLKGYVSSRLLEKIVETSTSDDEGKLADLFELLSKLS
                     PARYYKESFHDLARMAREDHPATRVFRRIFTDLHPNCRRKAISNFFVNFLLVGRDIRD
                     RKEAELGVHLPNFMVISPTMRCNLRCKGCYAAGYSKEDDLPLERIDRLIDEAKDLGMY
                     FFTFSGGECFVRPDLLDLWKKHHDCFFQVYTNGTLLDDATVGRLVKLGNVAPMVSVEG
                     SREETDMRRGRGMYDRVMETFGRLSEAGLLFGFSATCTRSSADHIASDAFLEEMLDHG
                     CKVGWFFQYIPTGDDPDLGYMATPTQRAALHRKVTEWRRKYPIFLGDFWNDGPFVDGC
                     MAGGERYLHVISSGDVEPCVFVHFAVDNINDKSLLEVIQSPFFRDIRDRQPYDDDNLL
                     CPCMVIDHPHVLREVVKTHGARATHPGSERILTELSRGLDDYSGSIHKLFDPLWEGGE
                     REQYLKSLEREDKQHPQGRFNKRLPTEQRTG"
     gene            complement(2288..3166)
                     /gene="secF"
                     /locus_tag="PLM30_01760"
     CDS             complement(2288..3166)
                     /gene="secF"
                     /locus_tag="PLM30_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOR53927.1"
                     /translation="MIFKIRKNIDFMGMRKVTLSLSLVLMVVSLGLLGFKGLNLGIDF
                     TGGNLAQVEFSHPVGVAEVREVLAEAGQGQAIIQAYSDNGVLIRMSSYDDKAAQEVLS
                     ALRSRFGEVEVLRLEKVGPVAGEQLRQQAAVALVIALGGILVYITVRFRFRFAVVSVA
                     ALVHDTVITLGAFSLLGREVSLPFIAAVLTIVGYSLNDTIVVFDRVRENWKGLRQRGM
                     ITLVNDSINETLSRTVNTSLTTLFPVVTLFLWGGPVIANFALALLIGIVAGTYSSVCI
                     AGALLGEWYLRSPEKA"
     gene            complement(3163..4578)
                     /gene="secD"
                     /locus_tag="PLM30_01765"
     CDS             complement(3163..4578)
                     /gene="secD"
                     /locus_tag="PLM30_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOR53928.1"
                     /translation="MLKKDRWRLILVVVVVVAALLFVFPIKGRVRLGLDLKGGAHILL
                     QAQGTAENPLTDDSVERLLAVLRNRIDQYGVTEPVIQREGKDRVIVDLPGVADPEAAL
                     ELIGKTALLEFRHVYESTGTVPPGPKRPNYDTEDEFLEAQARWEAVRGQFDRAADEMR
                     LKAEKDPDKVLARSEEGEAYLLGRAYVGGKDLEDAKTGFDNLGRPVVTLKFSSQGAKL
                     FDEATAANVGRQIAICLDNKVISAPVVQERISGGNAQISGRFSTAEAQRLAIMLRAGA
                     LPVAVEILENRSVGPTLGSDSINSGVRAGLVGAVLVLIFMLIYYRTLGLAADAALVVC
                     MLLVFAGLIGFRATLTLPGIAGIILTLGMAVDGNILIYERMKEEIAGGKTPQAAVDSG
                     FRKALTTILDANITTLIAAAVLFYFGTGPVRGFAVTLSIGIVAGVFSAVVVTRALLHI
                     FQSRGRGKVEAVPSGARGKRR"
     gene            complement(4747..5007)
                     /gene="yajC"
                     /locus_tag="PLM30_01770"
     CDS             complement(4747..5007)
                     /gene="yajC"
                     /locus_tag="PLM30_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOR53929.1"
                     /translation="MLLPLGVFVVIFYFLIIRPQKKRQRKHDEMLASIRNGDVVVTAG
                     GFYGTVKDIKDDSYIIEIADGVKARILKASIQMKKQDEHPAD"
     gene            complement(5146..5772)
                     /locus_tag="PLM30_01775"
     CDS             complement(5146..5772)
                     /locus_tag="PLM30_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOR53930.1"
                     /translation="MRVAEPTVERLVLYYRLLMQLHNEERRVVSSQEIGEMLGLKASQ
                     VRKDLSYFGEIGKRGVGYHVERLYRHVSDILSPDRVWKIALVGVGRLGEALLGHKALR
                     SEKFRLEALFDNDPAKIGQVICSVPCYDSEEMTEIIREKGIEVVILTVPAAAAQVSAD
                     RAVAAGTVKGILTFATTAISVPEEVLVYRVDISVELEKLLFFLKGRMA"
     gene            complement(5822..6118)
                     /locus_tag="PLM30_01780"
     CDS             complement(5822..6118)
                     /locus_tag="PLM30_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOR53931.1"
                     /translation="MNFREAPVLRVDRFLKLSRVVKRRSYAQEMITHGAVRVGGVPVR
                     SSRGLKPGDIIEVAFPRKILTIKVTSVDESLLKRGGEAFELIGQKQVSDDQRPW"
     gene            complement(6196..7386)
                     /gene="rpoD"
                     /locus_tag="PLM30_01785"
     CDS             complement(6196..7386)
                     /gene="rpoD"
                     /locus_tag="PLM30_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOR53932.1"
                     /translation="MTDVEKTPEELEVLDTADAEDAVDPTGEAEVAPREVAKYIESFK
                     ELLHVGREKGFVTYDDIEKHLPGEMMNEETLDTLYFNLSELGIDLVEVEKVKSEPRDD
                     SPSPPATEYSEEMGVLEDLPLSDPVRMYLREIGKISLLKPEEEVELAKLVEAGDPAGK
                     SHLVEANLRLVVSIAKKYIGRGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTYATWW
                     IRQAITRAIADQARTIRIPVHMVETINRLIRVSRQLVQRLGREPTAEEIAAEMAIGAE
                     RVEEIQRIAQEPVSLETPIGEEEDSQLSDFIEDKNLPSPEDAAAVQLLREQLEGMLEE
                     LTEREKEVLRLRFGLEDGHPYTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRSKKLR
                     DFLD"
     gene            complement(7391..9139)
                     /gene="dnaG"
                     /locus_tag="PLM30_01790"
     CDS             complement(7391..9139)
                     /gene="dnaG"
                     /locus_tag="PLM30_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR53933.1"
                     /translation="MDRDAVRTIKDRIDIVELIGESVQLRRAGKSFRGLCPFHAEKTP
                     SFYVSSERQTYHCFGCGRGGDLFSFVMEKEGMTFPEALALLADRAGVTLERPDGRRGR
                     SKGTAGAMEKALAFFRRSLAGPQGESARRYLERRGISVADAATFELGWAPPSWDSLKR
                     ELDASGIPAGDAMAAGLLAGEGTRVYDRFRGRVIFPIRDLQGRLVAFGGRLVDGEGAK
                     YINSPEGELYSKRRTLYLLNAAKSPIREKGRSILVEGYMDAIKLHMAGFPEAVASLGT
                     ALAGEQADLLKRFADQCLICYDSDAAGQEATIRGMYTLQKSGLDIRVVVLPKGKDPDD
                     VVSAPGGREHFLGLIKGAEPLPLFHIRVKSEELSDEGRKTTARREIIESLAGLPPLEA
                     NRHITQVAQGLGLLLPEMVDLLREARRSLGRAGKKGIPGPGSVYIYEDRGAPGGCKPV
                     DPWEASFCYLLWTDERKRAEVEPAQALALLSEEGAKAVALSILSGDSTEELRSRWAEA
                     GDDYPMRLISTGWGHCAKNGEREDMWDIVLSALGTMRSRDRFNALRERHARGEASPEE
                     MREYLELARKLKRRDR"
     gene            complement(9261..11222)
                     /locus_tag="PLM30_01795"
     CDS             complement(9261..11222)
                     /locus_tag="PLM30_01795"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOR53934.1"
                     /translation="MLAQIVWISGGAGLLALAFALFSSGRIRSFEVTNERVNELSGII
                     QRGSMAFLSREYRALVPFVVVVGAVLWLKIGHPMAVSFLLGAFCSGLAGLVGMRVATK
                     ANGKTAFAAGQGMNPALRIAFTGGTVMGMSVVGIGIIGIIICYGFFKDPNIITGFGFG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDIAGM
                     GADLFESYVNSIIAAMAIGLVAFGDTGVIYPLLLASLGIVSSVLGTFFVRVREEGDPQ
                     LALRIGTLVTGALMIAGSYFMTKAAFGDITLFWAVLSGVAVGVLIGLVTEYYTSSAYP
                     PVKGIAQASITGTATNILAGLGVGMKSTAIPVLMLCGSILVGVRFGGLYGIACAAVGM
                     LSITGMTLSVDAYGPIADNAGGIAEMSHMPPQVRKITDRLDAVGNTTAAIGKGLAIGS
                     AALTALALFAAYATAVGLEVIDINNPRVMVGLFIGGMLPFVFSALTIQAVGRAASQMI
                     DEVRRQFREIPGIMEGEGQPEYERCIEISTGASLKEMIIPGSLAVVSPVVVGLVLGAE
                     ALGGLLGGAIITGVMMAIFMSNAGGAWDNAKKFIEEGNHGGKGSEPHGAAVVGDTVGD
                     PFKDTAGPSLNILIKLMSVVALVLAPLFI"
     gene            complement(11382..12014)
                     /locus_tag="PLM30_01800"
     CDS             complement(11382..12014)
                     /locus_tag="PLM30_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOR53935.1"
                     /translation="MPFILAVSGFKDSGKTTLCKKLLPLLKERGLDVAYVKRTHEEVL
                     SPAGTDSGLLAQITDPVALWGSDGLRLEDGRQDMDPGRLAALYFPGRHLLLLEGGKEL
                     TLPKIWVGDPEDIPEGVLGVVAFYDRDNPCGKERHFCAGQEEELADLVSSMVRKAETS
                     PAEVYSHGRRIPVKSFVGEFIAGGVSGMLGSLKGDFDPEDGVSVHIRALP"
     gene            complement(12040..>12426)
                     /locus_tag="PLM30_01805"
     CDS             complement(12040..>12426)
                     /locus_tag="PLM30_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOR53936.1"
                     /translation="GFGWWWVGLPSPLLFAGAMFILAIIPFVGTPLVLLPGAAYLFAE
                     GRTGSAIFLAVWALLVVSAIDNFIRPLFISEGSKVHILIIFTGVVGGLATWGFLGIFI
                     GPLILSMFFFFLDGYRKIWQRMRHEN"
BASE COUNT         2266 a         4109 c         3627 g         2424 t
ORIGIN      
        1 cgtcgaggcc cgggtccagc tgcgcccggg cctcgcgggg gtcatccccc gagaaccctc
       61 tcatcctcag caggagagcg gtcaggggtg agcaggacac tctctccgcg aagatcctgg
      121 cgtcgtcgtc cacctcccgg agctccagcc gggagacggg gatctccccg atcactgccc
      181 ttccttttcc gcttccggtt ccgccgcgcc ttcctcggaa gtcacccccg ggagtcgggc
      241 ctcttgctcg ccctcgcgcc gttcaacact gtgccccagg gcttccactt tcggtccttc
      301 cacgccttcc tcggagcgct gaaccctgag ttcggcctcg tgccgttccc cgctgaatct
      361 caaggcttca accagggtcg acttttcgcc ctccaggtcc ttgatcctgg cttccttttc
      421 ctttacctgc cgtacatact tcgacctgct ctccatcgac gcgaaaaggg ataccagcca
      481 catcaggacc ccccccgcgg cgaagagcat cacttcccag atgccctggg ggaaagacag
      541 ctcccatatc aagaacctga ccaggacctc cccggtgttc tggaaggcgt ataccgccgc
      601 caccagcatt gccatggcga tggcaagggc gtaacttttc atctcctcac cccttttggg
      661 aatcgggtct tcactgccgt gaatagatta tcctgttctc tgctccgttg gcaatctttt
      721 gttgaaacgt ccctgggggt gctgcttgtc ctccctttcc aggctcttga ggtactgctc
      781 tctctcccct ccctcccaga gggggtcgaa aagcttgtgg atgctccccg aataatcgtc
      841 caaccccctg gaaagttccg tgaggatgcg ctcagaaccg ggatgggtcg cccgcgcccc
      901 atgggtcttt accacttccc tcagtacatg ggggtggtcg atcaccatgc aggggcagag
      961 gaggttatcg tcgtcatagg gctgcctgtc ccttatgtca cggaagaagg ggctctggat
     1021 cacctccagg aggctcttgt catttatgtt gtccacggcg aaatggacga agacgcaggg
     1081 ttccacatcc ccgctggaga tcacgtggag atacctttcg cccccggcca tgcacccgtc
     1141 cacgaagggg ccgtcgttcc agaagtcacc gaggaatatg gggtatttcc gccgccattc
     1201 ggtgaccttt ctatggaggg ccgccctctg cgtgggcgtg gccatgtaac ccaggtcggg
     1261 gtcgtcgccg gtggggatat actggaagaa ccacccgacc ttgcagccat ggtcgagcat
     1321 ctcctccagg aaggcatcgg aggcgatgtg atcggcgctc gacctggtgc aggtggcgct
     1381 gaagccgaag agcaaccccg cctcggagag cctgccgaag gtctccatca cgcggtcata
     1441 catcccgcgg cccctcctca tgtcggtctc ctcccgggaa ccctcgacgg agaccatggg
     1501 ggctacattc cccagcttca cgagccgccc cacggtagcg tcatccagga gcgtcccgtt
     1561 ggtgtagacc tggaagaaac aatcgtgatg ctttttccaa aggtccagca ggtcgggcct
     1621 cacgaaacac tccccgccgg agaaggtgaa gaagtacatg cccagatcct tggcctcatc
     1681 gatcagcctg tcgatcctct cgaggggcag gtcgtcttcc ttggagtacc ccgcggcgta
     1741 gcaccccttg catctcaggt tgcacctcat ggtggggctt attaccatga aattggggag
     1801 atggacgccc agttcagcct ccttcctgtc ccttatgtcc cttcccacga ggaggaagtt
     1861 gacgaagaag ttgctgatgg ccttgcgcct gcagttgggg tgaaggtcgg tgaagatcct
     1921 cctgaagacc ctggtcgccg ggtggtcttc ccgtgccatc ctcgcgaggt cgtggaaact
     1981 ctccttgtag taccgtgccg gggacagctt cgaaaggagt tcaaagaggt ccgcaagctt
     2041 gccctcgtcg tcggaagtgg aagtttccac gatcttctca agcaacctgg aggatacgta
     2101 acccttcagg cggtcaaaag aggctgcaga catgaaaatc cctccctctg gcgaatatct
     2161 gcattcgtgc gctctttcac cgtaatatag atcactatga aaatattata cctttcgccg
     2221 gcatccgtca aaaaagcgtg gggggccgtt ccccggcccc ccacgcctcg ttttcctgtc
     2281 ccccctgtca cgccttctct ggggatctca ggtaccactc gcccagcagg gctcccgcga
     2341 tgcagacgga actgtaggta ccggccacga tcccgatgag cagcgccagg gcgaagttcg
     2401 cgatgaccgg ccctccccag aggaaaaggg tcacgacagg gaaaagggtg gtcagggagg
     2461 tgttgacggt cctcgagaga gtctcgttta tcgagtcgtt aacgagggtg atcatccccc
     2521 gttgccgaag gcccttccag ttctccctga cgcggtcgaa gacaacgatg gtatcgttca
     2581 gggagtagcc cacgatggtc aggaccgcgg cgatgaacgg cagggacact tccctcccga
     2641 gcagcgagaa ggcccccagg gttattaccg tgtcgtggac aagggcggcg acgctcacca
     2701 cggcgaaacg gaaccggaac ctgaccgtga tgtagacaag tatcccccca agggcgatga
     2761 ccagggctac ggcggcctgc tgccgtagtt gctcacccgc cacggggccc accttttcca
     2821 ggcggagcac ttcgacctcc ccgaacctgg agcgaagcgc cgagaggacc tcctgggcgg
     2881 ccttgtcgtc gtaggaactc atcctgatga gcacgccgtt gtcgctgtag gcctggatga
     2941 tggcctggcc ctgacccgcc tcggcgagga cctcccggac ctcggccacc ccgacggggt
     3001 gggagaactc gacctgggcg aggttgcccc cggtgaagtc gatccccagg ttcaagccct
     3061 tgaaaccgag caatcccagg ctgacgacca tcaggacaag gcttagggag agggtcacct
     3121 tcctcatgcc catgaaatcg atgttcttcc tgatcttgaa tatcatcgcc ttttccccct
     3181 tgctcccgac ggcaccgctt caaccttgcc gcgccccctc gactggaaaa tatgcaggag
     3241 cgccctggtg acgacgacgg cgctgaagac gccggcgacg atccctatgg aaagggtgac
     3301 ggcgaaaccc ctaaccggtc cggtgccgaa ataaaaaagt accgccgcgg cgatgagggt
     3361 cgttatgttc gcgtcgagga tggtcgtcag ggctttgcgg aacccggaat ccacggcggc
     3421 ctggggggtt ttgcccccgg cgatctcctc cttcattctc tcgtagatca ggatattgcc
     3481 gtccacggcc atccccaggg tcaggatgat acccgcgatg ccgggcaggg tgagggtggc
     3541 ccgaaagccg ataaggcccg cgaagaccag cagcatgcag accacaaggg ccgcgtcggc
     3601 tgccagcccc agggtccggt agtagatcag catgaagatc agcacgagga cggcccctac
     3661 caggccggcc ctgacgccgg aattgatgga atccgaaccc agggtcgggc ccaccgaccg
     3721 gttctccagg atctccaccg cgaccgggag ggcgccggcc ctgagcatta tggccaggcg
     3781 ctgcgcctcc gccgtggaga accgaccgga gatctgggcg ttgcccccgc tgatcctctc
     3841 ctggaccacc ggcgccgaga tgaccttgtt gtcgaggcag atggctatct ggcggcccac
     3901 attggccgcc gtggcctcgt cgaaaagttt cgcgccctgg gagctgaact tcagcgtgac
     3961 cacggggcgc cccaggttgt caaaacccgt cttggcgtcc tccaggtcct tgccgcccac
     4021 ataggcccgg cccaggaggt aggcttcacc ttcttcgctc ctggccaaaa ccttgtccgg
     4081 gtccttttcg gccttgaggc gcatctcgtc ggcggcccgg tcgaactgtc cccggaccgc
     4141 ttcccaccgc gcctgcgcct ccaggaactc gtcctcggtg tcgtagttgg gccgcttggg
     4201 gccggggggg accgtccccg tcgattcgta gacgtgcctg aactccagca gcgccgtctt
     4261 gcctatgagt tccagtgccg cctcggggtc ggccaccccg gggaggtcga cgatgaccct
     4321 gtcctttcct tccctctgga tgacgggctc cgtgaccccg tattggtcta tcctgttccg
     4381 cagtaccgcg agaagacgct cgacgctgtc atcggtcagc gggttctcgg cggtgccctg
     4441 ggcctggagc aggatatggg cccctccctt gagatccagc cccaggcgga ccctcccctt
     4501 gatggggaag acgaagagca gggcggccac taccactacc acgacaagaa taagccgcca
     4561 acgatccttt ttgagcatgt gccttcctcc cctgttgaaa accttactct ccgcgggggc
     4621 ggcccataaa ccgccccgac ggccgagcct ttcataaaaa acggagcgga cgcccggcat
     4681 ccactccgaa ggaagccctg accggccctc tattgacgaa gaagaccatc gccggccgga
     4741 cggtggctag tcggccgggt gttcatcctg ttttttcatc tggatggaag ctttgaggat
     4801 cctcgccttg accccgtcgg cgatctcgat gatgtagctg tcgtccttga tatccttcac
     4861 ggtgccgtag aagccgccgg cggtgacgac cacgtccccg ttgcgtatcg aggccagcat
     4921 ctcatcgtgc ttccgctggc gcttcttctg gggcctgatg atcagaaaat agaagatgac
     4981 gacaaaaact ccaagcggca ggagcatact caagagtcca ctctgtccct gcaatagcaa
     5041 cgcctccctc tttatcctca cccttcgagg atcattttcg cactgaacat tctatcatga
     5101 catgaaagca atcggaacac taccgggcca gggatcccgg cgcacttagg ccatgcgccc
     5161 ttttaggaaa aagagcagtt tctccagttc gaccgagata tccaccctgt agacgaggac
     5221 ctcctcgggg acggatatcg ccgtggtagc aaaggtgagg atgcccttga cggtgccggc
     5281 ggccaccgcc ctgtcggccg agacctgggc cgcagcggcc ggaacggtca ggatcacgac
     5341 ctcgatcccc ttctcacgga ttatctccgt catttcctcg gaatcatagc agggaacgct
     5401 gcagatgacc tggcctatct tggcggggtc gttgtcgaaa agggcctcca gcctgaactt
     5461 ctcgcttctc aacgccttgt gccccagcag agcttcgccg agcctcccca cgcctaccag
     5521 ggcgatcttc cagaccctgt caggggaaag gatgtcgctg acatgcctgt agaggcgttc
     5581 cacgtggtaa cccacacccc gcttgccgat ctcgccgaag taggagaggt ccttcctcac
     5641 ctggctcgcc ttcagaccca gcatttcccc tatttcctgg gaggagacca ccctccgttc
     5701 ctcgttgtgg agttgcatga gaagcctgta gtaaagcacc aggcgttcaa cggtcggttc
     5761 cgctaccctc atcgttcctg cctcctcaag aatggctcgc cgggatggtt gctctaccct
     5821 ctcaccaggg tctctgatcg tcactcacct gtttctggcc tatcagttcg aaagcctccc
     5881 cgccccgctt caggagggat tcatccacgg aagtgacctt gatggtcagg atcttcctcg
     5941 ggaaggccac ctcgatgatg tcgcccggct tcagccccct ggaagagcgc accgggacgc
     6001 ccccaacccg taccgcaccg tgggtgatca tctcttgggc gtaactcctc cttttaacaa
     6061 cccgggacag cttgagaaaa cggtcaaccc tcaagacagg cgcctccctg aagttcatcc
     6121 gtgaacatta taacaaatga tttgtatcat tgcttaaata acggcaaacc tcgacagagc
     6181 gggccgatac cgtcttcaat cgaggaagtc cctgagcttt ttactcctgc tggggtgcct
     6241 gagcttccgc agggccttgg cctcgatctg ccttatcctc tcccgggtaa cgccgaagcg
     6301 tttccctacc tcctcgaggg tgtaggggtg gccgtcctcg aggccgaacc tcagccgcag
     6361 gacctctttt tccctctcgg tgagttcctc gagcatcccc tccagctgct ccctcagcaa
     6421 ctggaccgcg gcggcgtcct cggggctggg gaggttcttg tcctcgatga agtcgctcag
     6481 ctggctgtcc tcctcctcac cgatgggggt ctccagcgat accggctcct gggctatcct
     6541 ctggatctcc tctacccttt ccgcgccaat agccatctca gcggcgatct cctccgcggt
     6601 gggctctctc ccgagcctct ggaccagttg cctcgagacc ctgatgagcc tgttgatggt
     6661 ctccaccatg tggacgggga tccggatcgt cctggcctgg tcggcgatgg ccctggtgat
     6721 ggcctggcgg atccaccatg tcgcgtaggt gctgaacttg aaacccttgc ggtaatcgaa
     6781 cttctccaca gcacggatga ggccgaggtt gccttcctgg atgaggtcca aaaacagcat
     6841 gccccggccg atgtatttct tggctatgct gaccacgagc ctgaggttgg cctccaccag
     6901 gtgactcttt cccgcggggt cgccggcttc gacgagcttg gccagctcca cctcctcttc
     6961 gggcttgagc agggagatct tacctatctc cctcaggtac atcctgacgg ggtccgacaa
     7021 gggcaggtcc tccaggacgc ccatctcctc gctgtactcg gtcgccggcg gggacgggga
     7081 gtcgtcgcgg ggctcgctct tgaccttctc gacctccacc aggtctatac cgagttcgct
     7141 gaggttgaag taaagggtgt ccagggtctc ctcgttcatc atctcccccg gcaaatgctt
     7201 ctcaatatcg tcataggtca cgaaaccctt ctcccgtccc acgtggagca gttccttgaa
     7261 actctctatg tactttgcca cttctctcgg ggccacctcg gcctccccgg tcgggtcgac
     7321 ggcgtcttcc gcgtcggccg tatcgaggac ttcaagctct tccggggtct tctcaacgtc
     7381 ggtcatggct tcaacggtcc ctccttttga gttttctggc gagttcgagg tattccctca
     7441 tctcttcggg ggaggcttct cccctggcgt gcctctccct gagggcgttg aacctgtccc
     7501 ttgatctcat ggtcccgagg gccgaaagga ctatgtccca catgtcttcc cgctcgccgt
     7561 ttttggcgca gtgcccccac cccgtcgaaa tgagcctcat gggatagtcg tcaccggctt
     7621 cggcccacct cgacctcaac tcctcggtgc tgtcccccga gaggatcgac agggccacgg
     7681 ccttggcacc ctcttccgag agaagcgcca gcgcctgggc cggttccacc tcggccctct
     7741 tcctttcatc ggtccaaagg aggtaacaga aagacgcttc ccacgggtcc acgggtttgc
     7801 agccgccggg ggcgcccctg tcctcatata tatatacgct ccccgggccg ggaatgcctt
     7861 ttttcccggc ccggcccagg gacctccggg cttctctcag gagatcgacc atctccggca
     7921 gcagcaggcc caggccctgg gccacctggg tgatgtgcct gttggcctcc agtgggggca
     7981 accccgccag gctctcgatg atctccctcc tcgcggtggt ctttcgcccc tcgtccgata
     8041 attcctcgct cttgaccctg atatgaaaaa gcgggagagg ttcggccccc ttgatcaacc
     8101 ccaggaaatg ctcccgccca ccgggggcgc tgacgacatc gtcggggtcc ttgcccttgg
     8161 ggaggaccac caccctgatg tccaggccgc tcttttgcag ggtatacatc cccctgatcg
     8221 tggcctcctg ccccgccgcg tcggagtcgt aacagatgag gcactggtcg gcgaacctct
     8281 tgaggaggtc cgcctgctcc cctgccaggg ctgtccccag ggacgcgacc gcctcgggga
     8341 aacccgccat gtggagtttt atggcgtcca tgtagccttc gacgaggatc gagcggccct
     8401 tctcgcggat cggggacttc gccgcgttca gaagatagag ggtcctcctt ttactgtaga
     8461 gttcgccctc ggggctgttg atgtacttgg cgccctcgcc gtccacgagg cgacccccga
     8521 aggcgacgag ccttccctgg aggtccctga tggggaaaat gaccctgccc ctgaagcggt
     8581 cataaacccg cgtcccctcg ccggccagga gcccggcagc catggcgtcc ccggccggga
     8641 tgccggaggc gtcgagttcc ctcttgagcg agtcccagga ggggggcgcc caccccaact
     8701 cgaaggttgc ggcatcggca acgctgatgc ccctcctctc gaggtacctc ctggcgctct
     8761 ccccttgcgg ccccgccagg gaccgccgga aaaaggcgag ggccttctcc atggcgcctg
     8821 ccgtcccctt cgatcgcccc cggcgcccat cggggcgttc cagggtcacc cccgccctgt
     8881 cggccagcag ggccagcgcc tcgggaaagg tcattccctc cttttccatc acgaaggaaa
     8941 agaggtcgcc gccccggccg cagccgaaac agtggtaggt ctgccgttcc gaggagacat
     9001 aaaacgaggg cgttttctca gcgtgaaagg ggcaaagccc ccggaagctc ttgccggccc
     9061 tcctgagctg gaccgactca ccgatcagtt ccacgatgtc gatccggtct ttgatcgtcc
     9121 ttacggcgtc ccggtccaaa acagcaccac cttccccggg cggccccggc ggacaggccg
     9181 ggggagggca accggtcgcc ctcccccgcc gttatcgagc ctggtcagct tcttatgccc
     9241 cgtcgatgcg agggcggccc ctagatgaac aacggcgcca ggaccagggc taccaccgac
     9301 atcagtttta tgaggatgtt gaggctgggg ccggcggtat ccttgaaggg gtccccgacg
     9361 gtatcaccca cgacggccgc cccgtgaggt tccgaaccct tccccccgtg gttgccctcc
     9421 tcgatgaact tcttggcgtt gtcccaggcg ccgccggcgt ttgacatgaa gatggccatc
     9481 atgactcccg tgatgatggc cccacccagc agcccgccga gggcctctgc cccgagcaca
     9541 agacctacga ccacagggct gaccacggca agagacccgg ggatgatcat ctccttgagg
     9601 gaagctcctg tggatatctc tatgcagcgc tcgtactcgg gctggccttc accctccatt
     9661 atgccgggta tctcgcggaa ctggcggcgc acctcgtcga tcatctgaga ggcggcccgt
     9721 ccgacggcct ggatcgtcag ggcgctgaag acgaagggga gcatgcctcc gatgaaaagg
     9781 cccaccatga ccctggggtt gttgatatcg atgacctcca ggcccaccgc ggtggcatag
     9841 gcggcgaaga gggccagggc cgtgagtgcg gccgacccga tggccaaccc cttccctatc
     9901 gccgcggtgg tgttccccac cgcgtcgagc cggtccgtta tcttgcggac ctggggtggc
     9961 atatggctca tctcggctat gccgccggcg ttgtcggcga tggggccgta agcgtcgacg
    10021 ctgagggtca tcccggtgat ggagagcatg ccgacggccg cgcaggcgat gccgtagagg
    10081 ccgccgaacc tgacgccgac gaggatggaa ccgcagagca tcaagacggg gatggcggtg
    10141 gacttcatgc cgaccccgag gccggccagg atgtttgtcg ccgtccccgt gatggaggcc
    10201 tgggctatgc ccttcaccgg gggataagcc gaagaagtgt agtactccgt tacgagacct
    10261 atgaggacgc cgacggccac cccggagagg acggcccaaa agagggttat gtcaccgaag
    10321 gcggctttgg tcatgaagta ggaaccggct atcatgaggg ctcccgtgac gagggtccct
    10381 atcctgaggg ccagttgggg atccccctcc tcgcggaccc tgacgaaaaa ggtccccagc
    10441 actgacgaga cgatgccgag ggaggccagg agcagggggt agatgacgcc cgtgtcgccg
    10501 aaggccacca acccaatggc catggccgcg atgatggaat tcacgtagga ctcgaaaagg
    10561 tcggccccca tgccggcgat atcgcccacg ttgtcgccca cgttgtcggc gataacagcc
    10621 gggttcctcg ggtcatcctc ggggatgccc gcctcaactt tgcccaccag gtcggctccc
    10681 acgtcggcgg ccttagtgta tatcccgccg ccgaccctgg cgaagagggc tatggagctg
    10741 gccccgaagc cgaaaccggt gatgatgttg gggtccttga agaacccgta gcagatgatg
    10801 atgccgatta tcccgatgcc gacgacggac atgcccatga ctgtcccgcc ggtgaaggct
    10861 atcctgaggg ccgggttcat gccctgcccg gcggcgaagg ccgtcttgcc gttggccttg
    10921 gtggcgaccc tcatgcccac caggcccgcg aggccgctgc agaaagcccc gagaaggaag
    10981 gaaacggcca tggggtgacc gatcttgagc cagaggacag cgccgacgac cacaacaaag
    11041 gggaccagcg ccctgtactc cctggagagg aaagccatgg aacccctctg aatgatcccg
    11101 gacagttcgt tcacccgttc attggtgacc tcgaaagacc tgatgcgccc cgacgagaac
    11161 agggcgaacg ccagcgcgag cagcccggcc ccaccggata tccagacgat ctgtgccaac
    11221 attcaaggaa cctccctgca taatatgaag tcttgaaaga caaaagacgg cccgcctccc
    11281 gggcgggcgt caggtatatc ttatcgccca ccctttcggg gcgggcgacc atcattatag
    11341 acagcccggc cctttaccgc aaggacgttc ccggggcaac gtcagggtag ggccctgata
    11401 tggacgctca ctccgtcttc ggggtcgaag tcccccttga gggagcccag catcccggag
    11461 acgccgccgg cgatgaactc cccgacgaag gacttcacgg ggatcctcct cccgtggctg
    11521 tagacctcgg ccggcgaggt ctcggccttg cggaccatag atgacaccag gtcggccaat
    11581 tcctcctcct gcccggcgca gaagtgcctt tccttcccgc aggggttgtc cctgtcgtag
    11641 aaagccacca cgcccaggac cccttcaggt atgtcctctg ggtccccgac ccatatcttc
    11701 ggtagggtca gttccttccc gccctcaagg agaagaagat gcctgccggg gaagtagagg
    11761 gccgccaggc ggccggggtc catgtcctgt ctcccgtcct ccaaccggag gccgtccgat
    11821 ccccagagcg ccacgggatc cgtaatttgc gcgagcaggc cagaatcggt ccccgccgga
    11881 gagaggacct cttcgtgggt ccgcttcacg taggccacgt ccagccccct ctccttgagg
    11941 agggggagga gctttttgca gagggtggtc ttcccgctat ccttgaaacc actcacggcc
    12001 aaaatgaagg gcatgagcgt cacctcccgt cactcggttt cagttctcat gccgcatgcg
    12061 ctgccagatc tttctgtaac cgtccaggaa gaaaaagaac atcgaaagga tcaggggccc
    12121 gatgaagatc cccaggaaac cccaggtggc caggccgccg acgacgccgg tgaagattat
    12181 aaggatgtgg accttgctcc cctcggagat gaacaggggc cttatgaagt tgtcaatggc
    12241 gctcaccacg aggagagccc atacggccag gaaaatggcg gatcccgtcc tcccctccgc
    12301 gaagagatag gcggcccctg ggaggagcac caggggagtg cccacgaagg ggattatggc
    12361 caggatgaac atggcccccg cgaaaaggag ggggctcgga agcccgaccc accaccaccc
    12421 gaaccc
//
LOCUS       DAOSRG010000023        11927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5338_length_11927_cov_8.213780, whole genome shotgun sequence.
ACCESSION   DAOSRG010000023 DAOSRG010000000
VERSION     DAOSRG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5338_length_11927_cov_8.213780"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLM30_01810"
     CDS             complement(<1..814)
                     /locus_tag="PLM30_01810"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOR53937.1"
                     /translation="MERVSLIQRELIVRSASGLALGLAILGGIYLGNPYWFALCLLIC
                     MASLGEYYRMISQKGHVSRVVGFGAGISSLFAAVYSEDPAVLLPLLAAAPMLVLFVEL
                     ARKHILGESHGITSSGGTAAGILYIVLPWSFMVVLRDHMWGTYLLVTLFACTWSCDVF
                     SYLVGSRWGETPLCPSVSPKKTVEGFLGGLTGSLLCGGCLAYYWGMAPFSIILIALFC
                     GTFGQVGDLAESLLKREAGIKDTGDLIPGHGGMLDRFDSILVNSVLVFFVFGV"
     gene            complement(801..1514)
                     /gene="uppS"
                     /locus_tag="PLM30_01815"
     CDS             complement(801..1514)
                     /gene="uppS"
                     /locus_tag="PLM30_01815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOR53938.1"
                     /translation="MAGVPIERKAKTPSHVAIIMDGNGRWALKKGLPRLAGHREGAKA
                     VERVIRAAASTPGLDYLSLFAFSTENWRRPRAEVEGLLSLLKTYLRSKRDELVENKVR
                     MVFSGRIDQLAEDLQDEIALFEKATARGDQLTVVVCLNYGGRQEIVDGVNRLLRKGFA
                     GEVTEELISANLYRPGIPDPDLVIRTSGEMRISNFLLWGSAYAEFFFTDTLWPDFGEE
                     DLKKALDHYSLRERRYGAS"
     gene            complement(1496..2101)
                     /locus_tag="PLM30_01820"
     CDS             complement(1496..2101)
                     /locus_tag="PLM30_01820"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOR53939.1"
                     /translation="MMPERGAPLDHRAEAWERLTRAVNQCVKCPLSSGRRNVVFGEGD
                     RFTRLLFVGEAPGLEEDEQGIPFVGKAGQLLNRIFEAADIDRKDIFITNVVKCRPPGN
                     RVPSIEEMLACEDFLKAQIALINPSILVCLGMTSTKWILKTGESISRVRGKWFDWKGI
                     RVMPMFHPSFLLRNQSKKKGSPKDLTWMDIQEVRDRWLECR"
     gene            complement(2101..2943)
                     /locus_tag="PLM30_01825"
     CDS             complement(2101..2943)
                     /locus_tag="PLM30_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53940.1"
                     /translation="MVEETERILLDKVRVFAFVGPAGTGKSQRAQLVAQEFGVDFIID
                     DGLLIRKGQIVRGKSAKTEKNQVRAIRRALFEYADHRRSVAEFLFHSQPCSVMLIATS
                     SGMVSRILGNLGLPPPEKITRIEDVSSPQEISRAKKERSFKGRHVIPVSHIQVRKHFA
                     GKLVGRLKVLFDNSDGMEGEKTIVRPPFKFFGKVSIDPEAINQMARFIAERTAQVSTV
                     ASVQTRPANGGVSILMELGFRPGPRSMVILGESLRDRTSRAVSGITGLDVHRVDISFV
                     GVDT"
     gene            complement(2936..4045)
                     /gene="hisC"
                     /locus_tag="PLM30_01830"
     CDS             complement(2936..4045)
                     /gene="hisC"
                     /locus_tag="PLM30_01830"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOR53941.1"
                     /translation="MAWLRDLIRKDLSGVDKYRYGKPISELQRELGLEKIVRLSSNEN
                     PWPLPDSVKRAIQTSLENVNRYPDPRSYRLRRLLARKWGVSTGEIIIGAGTESILHSL
                     FHSIIAFGDGIVCPVPTYPLYRVAAIASGAAFVEVPVREGGTWEIKDIVDACTGRVKA
                     VVLSNPSNPLGTFLERERMLNLAHELDSRQILLVVDEAYAEYVDDPTYLSGIELFRDI
                     GRVVITRTFSKIYGLASLRVGYAIAPKTVADAFDKIHPVFEVSRVGQYAAHAALLEAE
                     FINGIRLKTISERNRITQSLSEIGVHTEQTRTNFILVKHRKSDEVYEGLLREGVIVRK
                     ASDLGLDGFLRVTVGLPEENEYFLSSIKKVLAKLG"
     gene            complement(4073..5356)
                     /gene="murA"
                     /locus_tag="PLM30_01835"
     CDS             complement(4073..5356)
                     /gene="murA"
                     /locus_tag="PLM30_01835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOR53942.1"
                     /translation="MNYKMLIHGGKPLDGSIDVQGAKNAALPVIASSLLLRDKTLRLR
                     RVPKLQDIFTMADLLRHLGASVHFKDNEMSIFVPEELNWETPKSLVRKMRASSLVLGP
                     LLARTKRAILPLPGGCAIGSRPIDLHLKGLARLGANIDLVHGAVHASAEALKGSKIYL
                     DFPSVGATENLLMAAVFAEGETVIENAAREPEIKNLIETLNLMGCPIEGAGTGTLHIQ
                     GIGDLEGGEIEIIPDRIEASTYLIAGAATGGDVTVSRVIPEHLDAITAKLEEAGVGIE
                     AGEDRVRAFPKDPMKSVSLKTLPYPGFPTDVQPQFTALMAISEGTSIIQEGVFESRFL
                     HVSELKRMGANIELQGNTAVVSGVRSLNGADVRATDLRAGAALVIAGLSTPDETSLFG
                     LGHIWRGYERMDEKIRSLGGQVKIVVSEENGDLKW"
     gene            complement(5361..6563)
                     /gene="wecB"
                     /locus_tag="PLM30_01840"
     CDS             complement(5361..6563)
                     /gene="wecB"
                     /locus_tag="PLM30_01840"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOR53943.1"
                     /translation="MTKERRDMPGKGVVCCVIGTRPEAIKMAPVILALKKSAHLSPYV
                     LATGQHSRMMRQPLDFFGIEPDRDLALMKEGQSLDYITTRVLVEVGEVLGEVKPFFTL
                     VHGDTTTTMAASLAAFYRRSRIGHVEAGLRSGNVNLPFPEELNRIIADRVAGWWFAPT
                     EGARDNLLREGCDPSRVVKTGNTVIDALLLATEKVSTPSYEPLSSIPSSAPVMLLTAH
                     RRESWGEPLKGICSAVARLMAENPDLWAIVPMHRNPEVRRVFREILSQEERVILCEPL
                     DYPDFIWSMKRSGLILTDSGGIQEETTILGTPTLVMRELTERPEAIEFGTSILVGTDP
                     DRILASARRVLMGNREGFSGTSAKGELPFGSGGASEKIVKTIEELFLIEARRYSSRGG
                     PDTIDPEG"
     gene            complement(6560..7441)
                     /locus_tag="PLM30_01845"
     CDS             complement(6560..7441)
                     /locus_tag="PLM30_01845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOR53944.1"
                     /translation="MWEKLVLFLIFLVWSLAVTPLSIFLSRQYGIVDEPGGRKRHPTT
                     TPRGAGVVIWVGYLLYAILAPNPGGEVRLIATGATAVFLFGYMDDMHPLPASWKLAAH
                     LAAAVTIAWSIPVQPAVKAAVILWVTGTTSAYNLVDGINGLSLAVFSLTALTGLFLTG
                     GSWWAVALGLSLGVLPWNYPFARTFLGDGGSTLLGFLCVSQFARDLPALLEGEGILPW
                     LAAMLLVGGVPVADTLYSLLRRAIKGISPFQPDRGHFHHLLVDRGLPLSVVLAILVSI
                     HSLSLWMAVLLLGRRTT"
     gene            complement(7474..8574)
                     /gene="upp"
                     /locus_tag="PLM30_01850"
     CDS             complement(7474..8574)
                     /gene="upp"
                     /locus_tag="PLM30_01850"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOR53945.1"
                     /translation="MRIALGSDHAGFNLKSCIEKSLHGLSVITGDFGAKGPENPFDYP
                     DAAFDVGYRVARKEYELGILVCGTGIGMSIAANKIPGIRAALAYDEETGKLAREHNDA
                     NILTLGGRKTDCESGIRIVRAWLSAEFQGSRHSDRIRKISSFEERSPSTGNEPEGSPR
                     LVTFDHPLIQHKIGIIRNKHTSVKVFRELVQEIAGLMVYETTRNLPLEPVTVETPITT
                     TTVSSISGKKIAIVPILRAGLGMVDGMLQLIPNAKVGHIGIYRDPGTLEPHEYYCKLP
                     GDIGERDIIIVDPMLATGGSAVAAIDLVKSKGGTKISLLSLIAAPEGVSRVQDKHPDV
                     TIFTAALDSHLDDHGYIVPGLGDAGDRLFGTR"
     gene            complement(8658..9596)
                     /gene="meaB"
                     /locus_tag="PLM30_01855"
     CDS             complement(8658..9596)
                     /gene="meaB"
                     /locus_tag="PLM30_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOR53946.1"
                     /translation="MDRIIEKALGGDHRSIARLITAVESEADSSRKIMQSVYPHTGKA
                     YLIGVTGSPGAGKSTFLDKLITSMMAAGQSIGVIAVDPSSPFTGGAVLGDRLRMQRHS
                     LDPGVFIRSMGSRGSLGGLSRGTYEAALILDACGKDVVLIETVGVGQSEIDIVKIADT
                     VALLLVPGMGDDIQIMKAGIMEIADVFIVNKADRDGSERVVREVNIMLDLQSGKEWRP
                     PVIPTIAESGEGVELAARSLSDHRRYLASSPEGERRKCRRHRLEVEEIVKREIAKIAE
                     KTWDDRISAGILEDLEYRRTDPYTVAGDLLSKVLSG"
     gene            complement(9640..9852)
                     /locus_tag="PLM30_01860"
     CDS             complement(9640..9852)
                     /locus_tag="PLM30_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HOR53947.1"
                     /translation="MSSVNIQALAALFLFVLCLFLSRMIVNISSGKWPGGALWVTYLR
                     VLLGFLFAAAITLGMYSFAGVDILKR"
     gene            complement(9987..10958)
                     /locus_tag="PLM30_01865"
     CDS             complement(9987..10958)
                     /locus_tag="PLM30_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR53948.1"
                     /translation="MEPVRVGVIGVGHLGFHHARVYTELLNTDVVGIMDTNPERAAAV
                     GELLRVPFYSDIEQFYRQARPDAVSVVVPTVQHFESAKRALSAGIHVLIEKPVTSTVE
                     EAVELLQQAAAQNLVLQVGHIERFNSAVQHVRNIIKEPLFLQSRRIGPFSSRISDVGV
                     VLDLMIHDIDIILSLVHSEISAISAMGRSVRSSLEDIATAQIAFENGTLAQILVSRVS
                     ERRLRQLEIMEPERFVTVNYETQDVSIHRCVQEKECGLVEVIEHPVFPKREPLKLELQ
                     HFVTCVREGKQPLVGIMDGKRALEVAISILKQIQAPAQMVENTAGTA"
     gene            complement(11007..11690)
                     /locus_tag="PLM30_01870"
     CDS             complement(11007..11690)
                     /locus_tag="PLM30_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOR53949.1"
                     /translation="MGETGGCLSRISQRLFGRDNGIPLVAPSQVHGTRMIEALPSTSL
                     PARPEGDALLVRRPGVFGSLRFADCIPVVGISGRPRPWVLLVHSGFVGTSRGVTGEAL
                     RETMKVTGSASLEETHFWVGPGIGPCCYSRKLDDPMTREGLKQLPGDSWRVEKGMAYF
                     DLPGAIITTLYDMNVPEGNITRIDQCTCCSSGDYYSYRKGDRTGKSFLLAGFREGFHK
                     PGLWWENKI"
BASE COUNT         2431 a         3619 c         3321 g         2556 t
ORIGIN      
        1 gcaccccgaa gacgaaaaac accaggaccg agttaacgag gatgctgtcg aacctgtcca
       61 gcatccctcc atgaccgggg atcaggtccc ccgtatcctt gatgcccgct tcccttttaa
      121 gcaacgactc ggcaaggtcg ccaacctggc cgaaggtgcc gcagaaaagg gcgataagga
      181 tgatcgaaaa aggcgccata ccccaatagt aggccaggca gcctccacaa agaaggctgc
      241 cggtcaagcc tccaaggaaa ccctcgacgg tctttttcgg actgaccgag gggcagaggg
      301 gtgtctcacc ccacctggaa cccacgagat aggagaagac atcgcacgac caagtgcacg
      361 cgaagagtgt caccagcagg tatgtacccc acatatggtc ccggaggacc accatgaagg
      421 accaggggag gacgatatag agtatgccgg cggcggtccc ccccgaactg gtgatcccgt
      481 ggctctcgcc gagaatatgc tttcttgcca actcgacaaa gagaacaagc atgggggccg
      541 ctgccagaag gggcaacagc accgcggggt cttcggagta cacggcggcg aacagggagg
      601 agatgccggc accgaaaccg accacccttg aaacatgccc tttctggctg atcatcctgt
      661 aatactcgcc cagggaggcc atgcagatga gcaagcaaag ggcgaaccag tagggattgc
      721 cgaggtagat ccctccaagg atcgccaggc ccagtgctaa accgctggct gaacgcacga
      781 tcagttccct ctgaataagg ctaactcgct ccataccttc gctccctgag ggaataatga
      841 tccagtgctt ttttaagatc ttcctcgccg aaatcgggcc aaagagtgtc ggtgaagaag
      901 aattccgcgt aagccgatcc ccatagcagg aagttgctta ttctcatctc tccacttgtc
      961 ctgatcacca ggtccgggtc aggtataccg ggccggtaga ggttggccga gatcaattcc
     1021 tcggtgactt ccccggcaaa accctttcgc agcagcctgt tgaccccatc gactatctcc
     1081 tgcctgccac cgtaattcag gcataccact accgtgagtt gatccccgcg ggctgtggct
     1141 ttctcgaaaa gggcgatctc atcctggaga tcctcggcga gttggtcgat cctgcccgag
     1201 aagaccatcc ttaccttatt ctcgaccagt tcgtctcgct tggacctcag gtaggtcttc
     1261 agcagcgaaa ggaggccctc gacctccgcc cggggacgtc tccagttttc cgtggaaaag
     1321 gcgaaaaggg acaggtaatc caggcctggg gtggaagcgg ccgcgcgtat gaccctttcc
     1381 acggccttgg caccctcgcg gtgaccggca agccgcggga gccccttctt gagggcccaa
     1441 cggccgttac catccattat gatcgccacg tgggaaggtg ttttggcctt tctttctatc
     1501 ggcactccag ccaacgatcc cttacctcct ggatatccat ccacgtcaag tccttggggc
     1561 tccctttctt cttcgactgg tttctcagaa gaaaactcgg gtggaacatg ggcatgaccc
     1621 ttatgccctt ccagtcgaac catttccccc gcaccctgga aatgctttca cccgttttca
     1681 gtatccactt ggtcgatgtc atcccaaggc agacgaggat cgacgggttt atcagggcga
     1741 tctgggcttt caggaaatcc tcgcaggcga gcatttcctc tatcgagggg acccggtttc
     1801 ctggaggtct gcattttacc acgttcgtta tgaagatatc cttcctgtct atatcggcag
     1861 cctcgaagat cctgttcaaa agctgacccg cctttcccac gaaagggatg ccctgttcgt
     1921 cctcttctag cccgggagct tcaccgacga aaagcaggcg ggtgaacctg tcgccctcac
     1981 caaagacgac atttcgcctc cccgaagaca gggggcactt cacacactgg ttcacggcac
     2041 gggtcagtct ctcccaggct tcggccctgt gatcgagggg ggcccccctc tccggcatca
     2101 ttaggtatcc actcccacga aggagatgtc cacccggtgc acgtccaacc ccgtgatgcc
     2161 cgagaccgcc cgggaggtac ggtcgcggag gctctctccc aaaatcacca tcgaccttgg
     2221 accgggtctg aatccgagct ccatcaggat agataccccc ccgttggctg gcctggtctg
     2281 taccgaggcc accgtagaga cctgtgccgt cctttcagcg ataaagcgtg ccatctggtt
     2341 gatggcctcg ggatcgatgc tgaccttgcc gaagaatttg aagggagggc gcacgatggt
     2401 cttctcgccc tccatgccat cggaattgtc gaaaaggacc tttagccttc ccaccagttt
     2461 tccggcgaag tgtttacgga cctgtatgtg ggagacggga atgacatgcc gtcccttgaa
     2521 ggacctttcc tttttcgccc gggaaatttc ctggggggag gagacatcct cgatccgggt
     2581 gatcttctca gggggtggca ggccgaggtt gcccagtata cgggacacca tccccgacga
     2641 cgtggctatg agcatcactg aacaaggctg ggagtggaaa agaaattccg ccacggacct
     2701 cctgtgatca gcgtattcga aaagggccct tcttatggcc cggacctggt tcttctcggt
     2761 cttggcgctt ttcccccgga cgatctgccc cttccgtatc aggaggccgt cgtcaatgat
     2821 gaaatccaca ccgaactcct gggccaccag ctgcgccctt tggctcttcc ccgtcccagc
     2881 ggggcccaca aaggcgaaga ccctgacctt atcaagaagg atcctttcgg tctcctcaac
     2941 caagttttgc cagcaccttt tttattgatg agagaaagta ctcgttctct tcgggcaacc
     3001 ccacggttac ccttaggaac ccgtccagcc ccaggtcact ggctttcctt acgatcactc
     3061 cttcgcgcaa gaggccttcg tagacctcat cgctcttcct atgcttgacc aggatgaagt
     3121 tcgtccgggt ctgctccgtg tggaccccga tctcggacag gctctgggtg atcctgttcc
     3181 tctcagagat ggtcttcaac cggatgccat ttatgaattc cgcctcgagc aaggcggcat
     3241 gggctgcata ttgccctacc ctgctgactt cgaagacggg gtggatcttg tcgaaggcat
     3301 cggccactgt tttgggggct atcgcgtaac ctacacgaag ggaggccagg ccgtagatct
     3361 ttgagaatgt cctggttatc acgaccctgc ctatatccct gaacaattcg atccctgaca
     3421 ggtaagtggg gtcatccacg tactcggcgt aggcctcgtc gacgaccagc aggatctgcc
     3481 gggagtccag ttcatgggcg aggttcagca tcctttcccg ttccaggaac gtccccagcg
     3541 ggttgctggg gttggacagg actacagcct tgacccttcc cgtacaagcg tcaacgatgt
     3601 cctttatctc ccaggtacct ccctcgcgga ctggtacttc cacgaaggcg gccccggaag
     3661 ctatcgccgc cacccgatag agcgggtagg tcggcacggg gcagactatc ccgtcgccga
     3721 aagcgattat ggaatggaat aaggaatgaa ggatgctctc ggtgccggcc ccgataatga
     3781 tctcccccgt ggaaacgccc cacttccgtg ccaggaggcg cctgagcctg tatgaacggg
     3841 gatcggggta gcggttcaca ttctcaaggc tggtctggat cgctctcttg accgaatcgg
     3901 gcagaggcca tgggttctcg tttgaactca accggacgat cttttccagc cctagttccc
     3961 tttgcagttc gctgatcggt ttaccgtaac ggtacttgtc gacacccgac aggtccttcc
     4021 tgatcagatc cctaagccat gccaattttc atacctcgct ttttgaacgc cctcaccatt
     4081 tgaggtcgcc gttctcctcg gacacgacga tcttgacctg tccgccgagg ctcctgatct
     4141 tttcgtccat cctttcgtaa ccccgccaaa tatgcccgag cccgaagagg gacgtttcgt
     4201 ccggcgtgga aagcccggca atgactagag ccgctcctgc tctcaagtcc gtggccctca
     4261 catcggcccc gttcagcgac cggacgccgg agaccacggc ggtgttcccc tggagttcaa
     4321 tattggcacc catcctcttc agttcgctca cgtggaggaa acgggattcg aatacaccct
     4381 cctggatgat gctcgtcccc tcggagatgg ccatgagggc cgtgaactgg ggctgcacat
     4441 ccgtaggaaa accggggtaa gggagtgtct tcagggatac gctcttcatg ggatccttcg
     4501 ggaaggccct gaccctgtcc tcacccgctt cgatgcctac tcccgcttcc tcgagtttgg
     4561 cggtgatggc gtcgagatgt tcagggatga cccgcgacac cgtcacgtcg cctcccgtgg
     4621 ccgccccagc tatcaggtag gtgctggcct cgatcctgtc ggggatgatc tctatctctc
     4681 caccctcgag gtcgccgatg ccctggatgt gcagggttcc ggtgccggcc ccttctatcg
     4741 ggcaacccat gaggttaagc gtctcgatca ggttcttgat ctccggttcg cgggcggcat
     4801 tttctatcac cgtctcacct tcggcaaaga ccgccgccat gagaaggttt tccgtggccc
     4861 cgacggaggg aaaatcgagg tagatctttg atcccttcaa cgcttcggcg ctggcatgca
     4921 ccgccccgtg gaccaggtcg atattcgcac ccagcctcgc gagcccctta agatgcaggt
     4981 cgatgggcct gctcccaatg gcacatccgc cgggaagggg aagtatcgcc cttttcgttc
     5041 gggccaggag tggacccagg acaagcgaag aggctctcat cttccggacc agtgatttgg
     5101 gcgtctccca gttgagttcc tcggggacga atatgctcat ctcgttgtcc ttgaagtgca
     5161 cgctcgcgcc caggtgcctc aggaggtcgg ccatggtgaa gatgtcctgc agcttgggga
     5221 ccctccgcag ccggagggtt ttgtctctca ggagcaagga ggaggctatc acaggaaggg
     5281 cggcgttttt tgctccctgt acgtcgatgg acccatccag gggtttacca ccgtggatca
     5341 acattttata gttcaagaga tcacccctca ggatctattg tatcaggccc gcctctgctc
     5401 gaataacgcc gggcctcgat caaaaagagt tcttcgatgg tcttgacgat cttctccgaa
     5461 gccccgccgg aaccgaaggg gagttctccc ttggccgagg taccggaaaa cccttcccgg
     5521 tttcccatga gaactcttct cgcggaggcc agaatgcggt ccgggtcagt cccgacgaga
     5581 atggaggttc caaactcgat cgcctcgggg cgttcggtca gttccctcat gaccagcgtg
     5641 ggggtcccaa ggatggtggt ctcctcctgg atccccccgc tgtcggtgag gataagaccg
     5701 ctcctcttca tcgaccagat aaagtcgggg tagtccagcg gctcgcagag gatgaccctc
     5761 tcctcttgcg aaagtatttc acggaagacc ctcctgacct ccgggttgcg atgcatgggg
     5821 actatcgccc aaagatcggg gttctccgcc attaacctcg ctacggcgga acagatcccc
     5881 ttgagaggct cgccccagga ttccctcctg tgagccgtca ggagcatcac tggcgcagag
     5941 gacggtatgg acgagagtgg ctcgtaggag ggagtggata ccttctctgt ggcgaggagc
     6001 aacgcatcga taacggtgtt ccccgtttta accaccctcg aagggtcgca cccctccctg
     6061 agtaggttgt cccttgcgcc ttccgtcggg gcgaaccacc aacccgccac gcgatcggcg
     6121 atgatcctgt tcagttcctc agggaagggc aggttcacat tcccgcttct caagccagcc
     6181 tcgacatggc cgatccggga ccgacggtag aaagccgcca gggaggcggc catcgtcgtg
     6241 gtagtgtcac catggacgag ggtaaaaaag ggctttactt cccccaggac ttccccgacc
     6301 tccacaagta ccctggttgt aatatagtcc aggctctggc cctccttcat gagggccagg
     6361 tcccggtcgg gctcgatgcc gaagaaatcg aggggctgcc tcatcatgcg actgtgctga
     6421 cccgtggcga gcacgtaagg cgaaaggtgg gcgctcttct ttagcgcgag tatgacgggg
     6481 gccatcttga tcgcttccgg ccgggtcccg atcacgcagc agacaactcc cttgccaggc
     6541 atatcccttc gttcctttgt catgtcgtcc ttcgccccag gaggaggacc gccatccaca
     6601 gtgataggga gtggatggag accagaatag ccaggaccac cgaaaggggc aaccccctgt
     6661 cgacaagcag gtggtggaag tgtccccggt ccggctggaa cggcgagata ccctttatcg
     6721 ccctccgaag gagggaataa agggtatccg ccacgggcac acccccgacg aggagcatgg
     6781 ccgcgagcca gggcaggatc ccttcacctt caagaagggc gggcaggtct ctggcgaact
     6841 gggaaacgca gagaaaacca aggagggtgc ttccaccatc accaaggaag gtcctggcga
     6901 aagggtaatt ccagggcagt acacccagag aaagcccgag agcaacagcc caccatgatc
     6961 ccccggtgag aaaaagaccc gttagagccg tcagggagaa aacggcgaga gaaaggccgt
     7021 ttataccgtc caccaggtta taggcgctgg tggttccggt gacccaaagt atgacagcgg
     7081 ccttcactgc gggctggacc gggatgctcc aggcgatagt gaccgcggcg gcgaggtggg
     7141 ccgccagctt ccacgaggcg ggaagggggt gcatatcatc catgtaacca aaaaggaata
     7201 ccgccgtcgc cccggtggca atgagcctga cctctccgcc cgggttggga gcgaggatgg
     7261 cgtaaagcag gtaacccacc cagatgacca ccccggcgcc ccttggtgtg gtcgtggggt
     7321 gtcttttcct tccgcccggt tcgtcgacga tgccgtactg ccgtgaaagg aatatcgata
     7381 ggggtgtcac cgccagtgac cagaccagaa atatcaggaa aaggaccagt ttttcccaca
     7441 atcccagaac cctccctacg ctttgggggt agcctacctg gtcccgaaaa ggcggtcgcc
     7501 cgcgtcccct aaccccggga ctatgtagcc atggtcatcg aggtggctgt ccagagcggc
     7561 ggtgaagatg gtcacatcgg ggtgcttgtc ttgaaccctt gaaacgccct cgggagccgc
     7621 aataaggctg aggagggaga tcttcgtccc ccccttgctc ttcacaaggt cgatggcagc
     7681 gacggcggag ccccccgtcg cgagcatggg gtcgacaatg atgatatccc tttcaccgat
     7741 atccccgggc aacttgcaat aatactcgtg gggctccagc gttccagggt ccctatatat
     7801 gccgatatgt cctaccttgg catttggtat cagctgaagc atgccgtcga ccatgcccaa
     7861 acccgctctc aatatcggta cgatggcgat cttcttgccg gaaatggagc ttaccgtggt
     7921 ggtcgttatc ggtgtctcta cagtgacggg ctccaggggc aggttcctgg tggtctcgta
     7981 gaccatgagg ccggcgatct cctgtacaag ttcacggaag acctttactg aggtgtgttt
     8041 gttcctgatg atgccgatct tgtgctgtat cagggggtgg tcgaaagtga caagccttgg
     8101 ggatccctcc ggctcatttc cagtagaggg acttctctcc tcgaaagagc tgatcttcct
     8161 gatccggtct gaatgtcttg agccctggaa ttcggccgag agccacgctc tgactatcct
     8221 gatccccgac tcgcaatcgg tcttccttcc gccaagggtc aatatgttag cgtcattatg
     8281 ctccctggcg agtttgcccg tctcctcatc ataggcaagg gccgcgcgga tacccgggat
     8341 cttgttggcg gcaatggaca tcccgatgcc ggtgccgcaa accaggatcc caagttcata
     8401 ttccttcctc gccaccctgt atccaacgtc gaaggccgcg tcagggtagt caaagggatt
     8461 ttccgggccc ttggcgccga aatcccccgt tatgaccgat aacccatgga gagacttttc
     8521 aatgcaactc ttgaggttga aacccgcgtg gtctgagccg agcgcgatcc gcattccatt
     8581 gccccctgtc atggtatcac tttatcatca tatcatccct gggcaggttg aaagggctgg
     8641 taccgttcca gggcctatca gcccgataac acttttgaaa gaagatcgcc agccacggtg
     8701 taagggtcgg tcctgcgata ctccaggtct tcaaggatgc cggcggatat gcgatcgtcc
     8761 caggtttttt cggcgatctt ggcgatctcc cttttgacta tttcctccac ttccaggcga
     8821 tgacgccggc attttctacg ctccccctcg ggggacgatg ccaggtagcg cctgtggtcc
     8881 gaaagggacc tggcggccag ctcgaccccc tcgccgcttt cagcgatggt ggggatcacc
     8941 ggaggtcgcc attccttgcc gctctgcagg tcgagcatga tgttgacctc tcgcacgacc
     9001 ctttccgagc catccctgtc agccttgttc acgatgaaaa catcggcgat ctccatgata
     9061 cccgctttca ttatctggat atcgtccccc atgcctggaa cgaggagcag ggcgacggtg
     9121 tcggctatct tgacgatgtc gatctccgac tgtccgacgc cgacggtctc tataagaacc
     9181 acatccttgc cgcaagcgtc gaggatgagc gccgcctcgt aggttccccg gcttaatcca
     9241 cccagggatc ccctggagcc catgctccgg atgaaaaccc ccgggtccag gctgtgtcgc
     9301 tgcatcctga gcctgtcacc gagcaccgcc ccccccgtaa agggactgga ggggtcaacg
     9361 gctattacac cgatgctttg tcccgcagcc atcatggagg ttatcagctt gtccaggaag
     9421 gtgcttttcc cagcgccggg gcttcccgtg acacctatca aatatgcttt cccggtgtgg
     9481 gggtaaacgc tttgcatgat cttcctggaa ctgtccgcct cgctttccac ggcggtgatc
     9541 agcctagcga tggaccgatg atcccccccg agggcttttt cgatgatcct gtccaaggtt
     9601 gacccattcc cttctttcga tgaactgttt tcgcggggct cacctcttca ggatgtcgac
     9661 cccggcaaag gagtacatgc ccagagttat ggccgccgcg aacaaaaagc cgagaaggac
     9721 cctcaggtaa gtgacccaaa gcgcccctcc aggccatttc cccgaagaga tattcacgat
     9781 catcctggaa aggaaaaggc agaggacgaa caggaagagc gctgccagcg cctggatatt
     9841 caccgaggac atgccgctac ccccaaatta tgaaaggtcc actccccggc tctgtattgt
     9901 aacagtaaaa ggggtcggat gccccgaagg gctcccaaca aaaaggccgc ctttcggcgg
     9961 ccggggtggg aaagataccc gttgatctag gccgtccccg cggtgttttc gaccatttgg
    10021 gccggcgcct ggatctgttt tagaatggat atggccactt caagggccct cttgccgtcc
    10081 atgataccca ccaggggctg cttgccctcc ctgacgcagg tgacgaagtg ctgcaactcg
    10141 agtttcagag gctctctttt ggggaagacc gggtgctcta tgacctccac taggccgcat
    10201 tccttctcct gcacgcaacg gtggatggat acgtcctggg tttcgtagtt gaccgtgaca
    10261 aacctctccg gttccattat ctccagttgt ctcaatcttc tttcggagac gcggctcaca
    10321 aggatctggg caagggttcc gttctcgaaa gcgatctggg cggtggctat atcctcgagg
    10381 gaagacctga cggacctacc catggctgaa atggccgata tctcggaatg gacaagggag
    10441 agaatgatat cgatatcatg gatcatcagg tccagcacaa cgcccacgtc gcttatcctg
    10501 gagctgaagg gcccgatcct cctggactga aggaagaggg gttccttaat gatgttcctc
    10561 acgtgctgca ccgcgctgtt gaatctttcg atatggccga cctggagcac caggttttga
    10621 gcggccgcct gctgtaacag ctccacagct tcttccacgg tggaggtgac aggtttctcg
    10681 atcagtacgt ggatcccggc ggagagagcc ctcttcgccg actcgaagtg ctggacggtg
    10741 gggaccacga cgctgacggc gtcggggcgg gcctggcggt agaattgttc gatgtctgaa
    10801 tagaaaggta ctctcaacaa ttccccgacg gcggcggccc tttcggggtt ggtgtccatt
    10861 atcccgacga cgtcagtatt gagcagctcc gtatagaccc gagcgtggtg aaaccctagg
    10921 tgtccaaccc ctataacgcc gacccttacc ggttccatcc gataacctcc cgccacgtca
    10981 cactttggct ttcttgcaag accccttcaa atcttattct cccaccaaag gcccggttta
    11041 tggaaacctt cacgaaaccc cgccagaaga aaagatttcc cggtccggtc tccttttcta
    11101 taagaatagt agtcgccgga agaacaacag gtacactggt cgatacgggt gatgttccct
    11161 tcggggacat tcatgtcata taaagttgta atgatcgcgc cggggaggtc aaaataggcc
    11221 attcccttct ccacacgcca ggagtccccg ggcagttgct tcaggccttc ccgggtcatc
    11281 gggtcatcga gttttcttga gtagcagcag ggtcctatcc cgggacctac ccagaagtgg
    11341 gtttcctcaa gggacgctga ccccgtgacc ttcatcgtct ccctcagggc ttccccggtc
    11401 actcccctgg aggtgcccac aaagccggaa tgcacgagga gcacccaggg ccgtggccta
    11461 ccgctgatgc ccaccacggg gatgcagtcg gcaaacctca gactgccgaa cacacccggt
    11521 cttctcacca ggagggcgtc tccctcgggc ctggccggaa gcgacgtgga tggcagggct
    11581 tcgatcatcc tcgtaccgtg cacctgggac ggggcgacca gggggatacc attatccctc
    11641 ccgaagaggc gctgggagat cctggaaagg caaccaccgg tttctcccat taaaggtcct
    11701 ttgaggaaca gcgtaactgt caaaatgtca tctatctggg ggggcaccag gtaccggatc
    11761 attccttgcc cttggaaaga aaccttttcc cagccccagt ccaggcccat tgtcaccggg
    11821 actccccttc cgccttgagg gagagcctgg tcatcatgta tcccagcagg taaaggctcc
    11881 cgcagatgat gatcttcttg tatcgcctgg aaacctcctc gagggcg
//
LOCUS       DAOSRG010000024        11058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5802_length_11058_cov_10.989094, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000024 DAOSRG010000000
VERSION     DAOSRG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_11058_cov_10.989094"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1112
                     /locus_tag="PLM30_01875"
     CDS             264..1112
                     /locus_tag="PLM30_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOR53950.1"
                     /translation="MAPISILAGKGNPMSPGTADEKGMIYKIARYAIHDGPGIRTTVF
                     LKGCPARCLWCCNPESQRREPELAKKVELCRTCPADALEWIGREITASDLLREVEPDR
                     PFWRRSGGGVTLSGGEPLFQPRFVKRFLQLCREKHIHTALESSLFGDWKFLEELMDLV
                     DFFQFDLKAMDPGEHLQLTGLDNRVILDNAARLLRSDKPLLTRIPLVPGCNDGESNLR
                     ELGAFLERHRPGAHLEVLPYHRMGVGRYEELGRPYPLPDTLPPTEEEMERALVILKDY
                     AIRVIN"
     gene            1126..3516
                     /locus_tag="PLM30_01880"
     CDS             1126..3516
                     /locus_tag="PLM30_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOR53951.1"
                     /translation="MPKTATERREQTVERIASMRKRMILEQPTELLPERALLVTQAYE
                     EHAAEPALLQRAYALQKVLSEMTLFMDDEELFVGHPSPQPRSPIVCPEFGARWILEDL
                     DNFATRSADAVGLTEENKILLRDCLTKWERSSLDAVVDRLISQEVKDALDEGMITVGG
                     RGTAQGNISINYSKLLARGLRGIIDEIDEKLRTDAPKGIDGTKKRTFWKAARISCEAV
                     IDFAHRYADLAARKAAGTNDTRRKEELLQISEILRQVPEFPATTFREAVQSVWLVYSV
                     LHIESDPHAILLGRVDQYLYPFYLKDKAEGRITDEDVVEILATLWIKCTAIIKLMDSV
                     TTRTFAGFPLFQNVTIGGQGSHGEDASNELSRLILDAALVARVTQPSIGFRYHNKVDP
                     DLLQKVAETIREGLGYPAIMNDGCIIPKHLIRGATLEEARNYCTNCVETDIEGMTDSR
                     AHSGYVNFPKCFLLAMNDGVDPESGRRIGPATGKLESFTSFDELFSAYKTQVAHFTKL
                     IVDAYDLVDGAHAVHTPEPFMSSLLDDCIERGMSRQEGGVRYNFSGIFGVGLASVADS
                     MAAVRRLCFDDRSVPPSKMLEALKNNFQGEETLLDACEKAPKFGNDDDYVDLIARDAA
                     HVFCSEVTQYPCMRGGFYIPELHSVSTHVYFGEVTGATPDGRPRGIAFSDGASPVGGR
                     DHKGPTAAVRSMTKIDHQEVLQGVLYNQKFSRSLLRKPGEVGAFTDYIRSYCDLGGHH
                     IQFNMISTEELRKAQENPSAFRDLIVRVAGYSAYFTELNRNTQDEIIARTEYEELS"
     gene            3547..>3932
                     /locus_tag="PLM30_01885"
     CDS             3547..>3932
                     /locus_tag="PLM30_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR53952.1"
                     /translation="MANSFNAPYADRINRLKEKVLTTYPEIDLENARILTQSFHETEG
                     EVFVLRKAKAFRRQCQEKTVEIWDDELIVGNAGSKIRGGILSADVCWSVLDKELDTIN
                     TRPYDKFHLRPEDRKIFEEEIRPYWKG"
     assembly_gap    3933..4032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4033..5713
                     /locus_tag="PLM30_01890"
     CDS             <4033..5713
                     /locus_tag="PLM30_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR53953.1"
                     /translation="RELRKIAEICRHVPAQPACTFHEALQSFYFYQIFLFMEQNAAAY
                     NPGRMDQYLYPYYKADHDAGRITPEEAQELLDCLWVKFSEQCLFQDGKSAEYSSGYPM
                     FQNLCAGGVDHQGRDAVNDLSYMILQATMDTQLYQPSLSVRYNMAKNPDAFLRKIVDL
                     ISLGTGFPAFHNDEVGIRMLMNKGVPLREALDWNPGGCVETNLAGKLRAYSALADINL
                     GSVVEYVLLGGKCRKTGAQVSIDTGDPRSFATWEEFEEALKAQLHYVIRVVVKASHII
                     DEICMDRPVPALSFSFEECITSAKDYAWGGAKYNTGNGIICIGVADLINSAAAVRHLV
                     YETKALTMGELLEALDRDFEGREETLRLCREAPKYGNDDPRVDDLAADLFSYIADEIE
                     NYRSKFGVMTPGILPVSGNTPFGKEVGALPSGRRAWKPLADGVSPNGGTDLNGPSAVL
                     KSVANIPHDRFVQGTLLNMKIDPEMLHTENGIIRFMALLKSLYSLGIYHAQFNVVDQS
                     KLIDAQKNPENYKGLLVRVAGYTAFFVELGKDVQDEIIARTVHGEKPSRTC"
     gene            5797..6765
                     /locus_tag="PLM30_01895"
     CDS             5797..6765
                     /locus_tag="PLM30_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOR53954.1"
                     /translation="MNILTKGTDGERVGRVLRIERSSIHDGTGLRTVVFLKGCPLRCL
                     WCSTPESQNPLPEKGYAEERCTLCGLCIENCPQGALSLDEAGKRIVTDRDKCVNCFEC
                     VRVCPSGAMKSYGGFMTVDEVVEEVEKDAVFYFHSGGGVTISGGEPLDQPEFVQALLR
                     ECQKHGIHRALETSFFAPWETIEPLLPLVNLLFVDMKHSMPEEHRRLTGVDNNLIVEN
                     ILKADASEYSFDLVIRTPLVPGLNDSEEALAKAAHFLKDLRKLKEVEFLAYHRLGTDT
                     YAHLGIPYPLNEIKSPSKDFMLSKARFFMSEAKGIPVAINGVPVNP"
     gene            6806..7747
                     /locus_tag="PLM30_01900"
     CDS             6806..7747
                     /locus_tag="PLM30_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOR53955.1"
                     /translation="MERKDFALAFLVVTIWGGNLIVIKLGLNDVPPMLLAALRYIFTA
                     LPAIAFIKPPRLEWRYTIAYGMTIGFGQFSCLFYAMYIGMPAGLSSILLQTQAFFTIF
                     FAAFFFREKLEARQMIGLVTALLGLFLIGGIKSPAGIPPVSFTAFALTMAAAAFWGLS
                     NIVVRMAAKHTASRGEKLNTLSLVAWSSLVPPLPLLGLAVLMDTPRELIGAVAHMNRI
                     SLFAILYLAYLGTIFGSSRWSGLLAKYPVGKVAPFSLLTPLTSLVMARFILGEELSLH
                     QYLGSMVVLLGLWIVNFNGRLKSEGQVQPGRGERTST"
     gene            complement(7866..9191)
                     /locus_tag="PLM30_01905"
     CDS             complement(7866..9191)
                     /locus_tag="PLM30_01905"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR53956.1"
                     /translation="MELTASTYLIMLLGLLFGGLFLGFPMAFTLGGSAVLAALIYSGP
                     GMLSFAASQLYSGMLSIGLISLPLFVFIACLFERSGIAEEMFECFRQWLAGVHGSVAM
                     ATVVVCTIIAAFSGVSTAGVVTMGIIALPLMLRYGYNKSIASGSIMAGGALAILIPPS
                     VSFIVYGMLARVSIGKLFAGGIVPGLILALGYLVYINLRCRLNPELGPPVPVGDRVSF
                     QEKVRMTRGLLVPFLLILSILGTILIGAASPSEAAAIGAGVALLCVAAKRKLTLALLW
                     ESCDRTFRICNMVMWIIVGAKLFATMFVTVGAADLIRQFFLNLNVTPLVMVMIMQMTY
                     IIMGCVTEEITMMCLTLPIYAPILASYGFDPVWFGVLFMINMQIGYLSPPFGYCLFYL
                     KGVAPPEITTMDLYRSVWPFILIQIAVVLLVIFFPQLTLWLPSNVLGLR"
     gene            complement(9194..9691)
                     /locus_tag="PLM30_01910"
     CDS             complement(9194..9691)
                     /locus_tag="PLM30_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOR53957.1"
                     /translation="MKYFIRFIGKLNTFLGKTFCFLILPLIGILCYEVISRYVFNAPT
                     IWAHEISGHIFGAYAIIGGGYVLLHREHIKSDVLYALFSERSKAIVDLITYPFFFLLM
                     GVIFYHGCKMAYTSVLYRETTVSFLRSPVYPVKLFIPAASMLMILQGIAQFVETLYGL
                     FEKKV"
     gene            complement(10319..>11058)
                     /gene="dctP"
                     /locus_tag="PLM30_01915"
     CDS             complement(10319..>11058)
                     /gene="dctP"
                     /locus_tag="PLM30_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOR53958.1"
                     /translation="NPLFLLNNTWELHQYVEKQNDGRILALLREELAKYGDVHVVGPA
                     YYVLEMIMNSKVPIDSIDKIKGMKFRSGDVPVANALGALGASVTWSPGEEIYTNLSSG
                     VIDACTYCGPADSIAMGFHEVAKYWLRTPLMGPVNVDYFIVNGKVWRELPDDLKAVVE
                     SALAAGNAFAEFQGSADNGKAWGQAKDLGVTIVDWSEEDALKWNATVASFLPDFGKDA
                     ASKEAIEVLKAYLQEYRPELAKALMEQ"
BASE COUNT         2418 a         3333 c         3193 g         2014 t
ORIGIN      
        1 acaggagcga cctgatcccg ctcacctact acgccaacga cctgttgggc tagatgggcc
       61 ggccccggca tcggggacaa gacgcggcgg ctcctaaacc cgataaacgc cggggcccca
      121 ttgtgctata atgacttacg ttgaagaaag atttttggga gaggggcgag gactctcctg
      181 ttttggtata atcatattgg taatgccaag gacttatcag aatgactttc aggggaaggt
      241 cttgttgaaa ccggcttctg tccttggcgc cgatctcgat actggcaggg aaggggaacc
      301 ccatgtcgcc tggaaccgcc gacgagaaag gaatgatcta caaaattgcc cggtacgcca
      361 tccacgatgg accggggatc cgcaccaccg tttttttgaa aggctgcccc gcacgctgcc
      421 tttggtgctg taaccccgag tcgcagcgcc gggaaccgga actggcgaaa aaggtagagt
      481 tgtgcaggac ctgtcccgcc gacgcgctgg aatggatagg tcgggagatc accgcctctg
      541 acttactccg agaagtagaa cccgaccgcc ctttttggcg aaggtcggga gggggcgtga
      601 ccctttccgg tggtgaacct cttttccaac cccggttcgt gaagcgattc cttcaacttt
      661 gccgggaaaa acacattcac accgccttgg agagcagcct tttcggcgac tggaagttcc
      721 tggaagaatt gatggatctc gtcgatttct tccagtttga cttgaaggcc atggatcccg
      781 gggagcacct tcaattgacc ggtctcgaca accgggtcat tttggataat gccgccaggc
      841 tcctcaggag cgataaaccg ctcctgacaa ggatacccct cgttcccggt tgcaacgatg
      901 gggagtccaa tcttcgtgaa ctgggggctt tcctggaacg gcaccgcccc ggggcccacc
      961 tggaggtcct accttaccac cgcatgggag tcggccgata cgaggagttg ggcagaccct
     1021 accccttgcc ggatactctc ccgccgacgg aggaagagat ggagagggcc ctggtgatcc
     1081 tgaaagatta tgctatccgg gttatcaact agggaggtcg atagcgtgcc caagacagcc
     1141 acggaaagac gcgaacagac cgttgaacgg atcgcatcga tgagaaagag gatgattctc
     1201 gaacagccca cggaactact ccctgaaagg gccctcctgg tcacccaggc ctacgaggag
     1261 cacgccgccg agccggcctt gctgcagcgg gcctatgccc tgcagaaggt gctctcggaa
     1321 atgacccttt tcatggatga tgaggaactt ttcgtcggcc atccttcgcc gcaaccccgg
     1381 tcacccatcg tctgcccgga attcggcgcc cgctggatcc tggaggacct ggataacttc
     1441 gccacccgca gcgccgacgc cgtggggctc acggaagaga acaagatcct tttgagagac
     1501 tgcctgacca agtgggaacg gagttccctc gatgccgtcg tcgaccggct tatctcccag
     1561 gaggtcaagg acgccctcga tgagggaatg atcactgtcg gtggccgagg gaccgcccag
     1621 ggcaatatct ccataaacta cagcaagctg ctcgccaggg gcctccgggg gatcatcgac
     1681 gagatcgacg aaaaactccg tacggatgcg cccaagggta tcgacgggac gaagaaacgg
     1741 accttctgga aagccgcccg gatcagctgc gaagccgtga tcgatttcgc ccaccgctac
     1801 gcggacctgg ccgcccggaa ggccgccggg accaacgaca cccgaaggaa ggaggaactc
     1861 ctccagataa gcgaaatcct gaggcaggtc cccgagttcc cggcgacgac cttcagggag
     1921 gctgtccaat cggtatggct cgtctattcg gtgcttcaca tcgagtcgga cccccacgcc
     1981 atactcctgg ggcgcgtcga ccagtacctg tatcctttct acctgaaaga caaggctgaa
     2041 ggccgcatca ccgatgagga cgtggtggag atcctggcca cgctctggat aaagtgcacc
     2101 gccatcatca agctcatgga ctcggtgacg acccgtacct tcgccggttt cccgctcttc
     2161 cagaatgtga ccatcggcgg ccaggggagc cacggggaag acgcctccaa cgagctatcc
     2221 aggctcatcc tcgacgcagc cctcgtcgcc cgcgtcaccc agccctccat cggcttccgc
     2281 taccacaaca aggtggaccc ggacctgctg caaaaagtgg cagagacgat ccgagaggga
     2341 ctgggctacc ccgccatcat gaacgacggg tgcataatcc ccaagcacct gatccggggg
     2401 gccaccctgg aagaggcccg caactactgc accaactgcg tcgagaccga tatcgagggg
     2461 atgaccgatt cccgcgccca ttcgggctat gtcaacttcc ccaagtgctt cctgctggcg
     2521 atgaacgacg gcgtcgaccc cgagtccggc cgccgaatcg gacccgcgac ggggaaactc
     2581 gagtccttca cctccttcga cgaacttttc tcggcctaca agacccaggt ggcccatttc
     2641 accaagctca tcgtcgatgc ctacgacctg gtcgacgggg cccacgccgt ccatactccc
     2701 gagcctttca tgtcgagcct cctcgatgac tgcatcgaac ggggaatgag ccgccaggaa
     2761 ggcggggtcc ggtacaactt ttcggggatc ttcggcgtcg gcctcgcctc cgtggccgat
     2821 tccatggcgg cggtgcgccg gttgtgcttc gatgaccgat cggtgccccc ttcaaaaatg
     2881 ctcgaagcgc tgaaaaacaa cttccagggc gaggaaaccc tcctcgacgc ctgcgagaag
     2941 gcgcccaagt tcggcaacga tgacgactac gtcgacctca tcgcccgcga cgctgcccat
     3001 gtcttttgca gcgaggtgac ccagtacccc tgcatgaggg gcggtttcta catccccgag
     3061 cttcactccg tctcgactca cgtctacttc ggtgaggtca ccggggccac tcccgacggc
     3121 cgccccaggg gcatcgcctt ctccgacggc gcctcccccg tgggtggcag ggaccacaag
     3181 ggccccacgg cggcagtgcg gtcgatgacc aagatcgacc accaggaagt cctccagggg
     3241 gtcctttaca accagaagtt ctccaggagc ctcctgagaa agcccggcga agtgggggcc
     3301 ttcaccgatt acatccgctc ctactgcgac ctgggaggcc accacatcca gttcaacatg
     3361 atctccaccg aggaattgcg gaaagcccag gagaacccct cggccttccg cgacctgata
     3421 gtacgggtgg cagggtacag cgcctatttc accgagctca accgcaacac ccaggatgag
     3481 atcatcgccc gtacggagta cgaggagctc tcctaatttc aagaaaggga tctggaggtg
     3541 tcgataatgg ctaacagttt caatgcaccc tacgcggacc ggataaacag gttaaaggag
     3601 aaggtgctga ccacctaccc cgagatcgac cttgagaacg cgaggatcct cacacaaagc
     3661 ttccatgaaa ccgaggggga ggtctttgtc ctgaggaagg ccaaggcttt ccggcgccag
     3721 tgccaggaga agaccgtgga gatctgggac gatgaactga tcgtaggcaa cgccggcagc
     3781 aagatccgcg gcggtatcct ctccgccgac gtctgctggt ccgtactcga caaggagttg
     3841 gacacgatca acacccgtcc ctacgacaaa ttccacctcc ggccggagga ccggaagatc
     3901 ttcgaggagg agatccgccc ctattggaaa ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nngagagagc tccggaagat cgccgagatc tgcaggcacg tcccggccca
     4081 gccggcctgc accttccacg aggcgctgca gtccttctac ttttaccaga tcttcctgtt
     4141 catggaacag aacgccgccg cctacaaccc cggcaggatg gaccagtacc tgtaccccta
     4201 ctacaaggcc gatcacgacg cgggtcgcat cactcccgag gaagcccagg aactgctgga
     4261 ttgcctttgg gtcaagttct ccgaacagtg cctgttccag gacggcaagt cggccgagta
     4321 ctcttcgggg taccccatgt tccagaacct ttgcgccggc ggtgtggacc accagggcag
     4381 ggacgccgtc aacgacctct cctacatgat cctccaggcg acgatggata cccagctcta
     4441 ccagccctcg ctctcggtgc ggtataacat ggccaagaac cccgacgcct tccttcgcaa
     4501 gatcgtcgac ctcatcagcc tggggacggg ttttcccgcc ttccacaatg acgaggtcgg
     4561 catcaggatg ctcatgaaca agggggtccc ccttcgggag gccctcgatt ggaaccccgg
     4621 gggatgcgtc gagacgaacc tggccgggaa gctccgggcc tattccgcct tggctgatat
     4681 caacctcggg agcgtggtgg agtacgtgct cctcggcggg aaatgccgca agacgggcgc
     4741 ccaggtgagc atcgacacgg gcgaccccag gtccttcgcc acctgggagg aattcgagga
     4801 ggccctcaag gctcagctcc attacgtcat ccgggtcgtc gtcaaggcca gccatatcat
     4861 cgacgagatc tgcatggacc gccccgtgcc ggccctgtcc ttcagcttcg aggaatgcat
     4921 caccagcgcc aaggattacg cctggggcgg agccaagtac aacacgggca acggcatcat
     4981 ttgcatcggc gtcgccgacc tgatcaacag cgccgccgcc gtgcgccatc tcgtatacga
     5041 gacaaaagcc ctcaccatgg gcgagctcct cgaggccctg gaccgcgatt tcgaaggccg
     5101 cgaggagacc ctccggctct gccgggaggc gccgaagtac ggcaacgacg acccccgcgt
     5161 cgacgacctg gcggcggatc ttttctccta catcgccgac gagatcgaaa actaccgcag
     5221 caagttcggc gtaatgaccc ccggcatcct tcccgtctca gggaacaccc ccttcgggaa
     5281 ggaagtgggc gccctgcctt cgggacggcg ggcgtggaaa cccctcgccg acggggtcag
     5341 tcccaacggg ggaacagacc tgaacggccc cagcgccgtc ctcaagtccg tcgccaacat
     5401 cccccacgac cggttcgtcc aggggacgct gctgaacatg aagatcgatc ctgaaatgct
     5461 ccacaccgag aacgggatca tccggttcat ggccctgctg aagagccttt actccctggg
     5521 gatctatcat gcccagttca acgtcgtcga ccagtccaaa ctgatcgacg cccagaagaa
     5581 ccccgaaaat tataaggggc tccttgtccg cgtcgccggt tacacggcct tctttgtcga
     5641 actgggcaag gacgtccagg acgagatcat agccaggacg gttcacggag agaaaccctc
     5701 caggacctgc tgatttagca attccgaggc ggcggagttc ccgcttcgcc gccccggtca
     5761 cttccgatcc aaggatcgaa ccaggggagt catgccatga atatccttac aaaagggacc
     5821 gacggagaac gggtcggcag ggtgctgcgg atcgagaggt cctccatcca tgatgggacg
     5881 gggctgagaa cggtcgtttt tttaaagggc tgccccctgc gctgtctctg gtgttccact
     5941 cctgagtctc aaaaccctct gcccgagaaa ggctacgccg aggaacgctg caccctgtgc
     6001 ggcctctgca ttgaaaactg tcctcagggg gccctgagcc tcgatgaggc gggcaagagg
     6061 atcgtcaccg accgggataa gtgcgtcaat tgtttcgagt gtgtcagggt ctgtccctcc
     6121 ggtgcgatga aaagctacgg gggcttcatg accgtcgacg aggtggtgga ggaggtcgaa
     6181 aaggatgccg tcttctactt ccattccggc ggaggcgtca ccatcagcgg cggggaacct
     6241 ctggaccaac ctgagttcgt ccaggccctc ctgcgggaat gccaaaagca cggtatccac
     6301 cgcgccctgg aaacgagctt tttcgccccc tgggaaacga tcgaacctct gctccctctg
     6361 gtgaacctgc ttttcgtgga tatgaagcac tccatgcccg aagaacaccg acgccttacc
     6421 ggggtcgaca acaacctgat cgttgaaaac atcctcaagg ccgatgcctc ggagtattcc
     6481 tttgacctcg tcatccgtac ccccctggtc ccagggctga acgattcgga agaggccctc
     6541 gccaaagcgg cgcattttct caaagacttg agaaaactga aagaggtgga atttttggcc
     6601 tatcaccgtc ttggcaccga cacctacgct cacctgggga ttccttatcc cctgaacgag
     6661 ataaaaagtc cgagcaagga tttcatgcta tccaaggcaa ggtttttcat gtcagaggcc
     6721 aagggaatcc ccgtcgcgat caacggagtc cccgtaaacc cctaacaatc tgaacacata
     6781 ccttagcttt tggggaggac ccgccatgga gagaaaagat tttgccctgg ccttcttggt
     6841 cgtgaccata tggggcggca acctgatcgt gatcaaattg ggtctcaacg acgtaccgcc
     6901 catgcttctc gccgccctgc gctacatctt cacggcgcta ccggccatcg ctttcataaa
     6961 accgccccgg ctggagtggc gctacactat cgcctacggc atgaccatcg gtttcgggca
     7021 gttctcctgc ctcttctacg ccatgtacat cggcatgccc gcggggctct cctcgatcct
     7081 tctccagacc caggcctttt tcactatctt cttcgccgct ttcttcttca gggaaaaact
     7141 cgaggcccga cagatgatag gcctcgtgac ggccttactg ggccttttcc tgatcggcgg
     7201 catcaagagc cccgccggca tacctcccgt ctctttcacg gccttcgccc tgaccatggc
     7261 cgccgcggct ttttggggac tatcgaatat cgtcgtacgg atggccgcca agcataccgc
     7321 atcccgaggg gaaaaactga acaccctgag cctcgtggca tggtccagcc ttgtgccacc
     7381 cctgccactc ctcggcctgg ccgtgctgat ggacactccc cgcgagctca tcggcgcggt
     7441 ggcgcatatg aaccgcatct ccctcttcgc catcctttac ctggcctacc tggggaccat
     7501 tttcgggtct tcccggtgga gcggcctgct ggcgaagtat cccgtgggga aggtcgcacc
     7561 cttttccctg ctaacgccgc tgaccagcct tgtcatggcc aggttcatcc tcggggaaga
     7621 gctgtccctg caccagtacc tcggcagcat ggtggtcctc ctcgggttat ggatcgtcaa
     7681 cttcaacggg cggctaaaaa gtgaggggca agttcaaccc ggccgggggg agcggacctc
     7741 gacataagcg caacctccgg gtacggcgtc aacacggggc ttcaccgatc agcggttggg
     7801 ccgggtggaa gaaaagagcg cccggggact tcggtccccg ggcgaagttt taccggtatc
     7861 gacccctagc gcagtcccag gacgttgctc gggagccaca gggtgagctg ggggaagaag
     7921 atgaccaaca ggaccacggc gatctggatc aggatgaagg gccaaaccga tcggtaaaga
     7981 tccatcgtcg tgatctccgg cggggccacc ccctttaggt aaaagaggca gtaaccgaag
     8041 gggggcgaca ggtagccgat ctgcatgttg atcatgaaaa ggacgccgaa ccagacggga
     8101 tcgaacccgt aactggccag gatcggcgcg tagatgggca gggtcaggca catcatcgtt
     8161 atctcttccg tgacgcaccc catgatgatg taggtcatct gcatgatcat gaccatgacc
     8221 aggggagtga cgttgaggtt caggaaaaac tggcggatca ggtcggccgc ccccacggtg
     8281 acgaacatgg tggcgaagag cttggcaccg acgatgatcc acatgaccat gttgcagatc
     8341 cggaaggtcc tgtcgcagga ctcccacaat agagccaagg tcagttttct cttggcggcc
     8401 acgcaaagaa gggccacgcc ggcaccgatg gcggctgcct cggagggtga agcggcgcct
     8461 atcaagatcg tgcccaagat ggaaaggatc aggaggaagg gtacaagaag ccctcgcgtc
     8521 atcctcacct tttcctggaa agagaccctg tctccaacgg gaacgggagg accgagttcg
     8581 gggttcaaac ggcagcgaag gttgatgtag accaggtagc ccagggccag gatcaggccc
     8641 gggacaatgc cgccggcgaa gagcttgcct atggaaactc tggccagcat gccgtaaacg
     8701 atgaaggaca cgctgggggg gatcaatatg gccaaggcgc cgccggccat gatggagccc
     8761 gaagcaatgg atttgttata gccgtaacgg agcataaggg gcaaggctat gatacccatg
     8821 gtcaccacac cggccgtcga cacccctgaa aaggccgcga tgatcgtgca gaccacgacg
     8881 gtggccatgg ccaccgaacc atggacgccg gcgagccact ggcggaagca ctcgaacatt
     8941 tcctcggcaa tgccggacct ttcgaagagg caggcaataa agacgaaaag aggcagagag
     9001 atcaaaccta tggagagcat gccgctgtag agctggcttg ccgcgaaaga gagcatgccg
     9061 ggccccgaat agatgagggc cgccaggacg gcgctgccac ctagggtgaa ggccataggg
     9121 aagccgagga agagtccgcc gaaaagaagc ccgagaagca tgatgaggta agtggacgcg
     9181 gtcagctcca tgatcacacc ttcttctcga aaaggccgta gagagtctcg acgaactggg
     9241 cgatcccctg aaggatcatc agcatcgaag cggcgggaat gaagagcttc acggggtaaa
     9301 cgggcgaacg gaggaaggag accgttgtct cacggtatag gaccgacgtg taggccatct
     9361 tgcagccgtg atagaagatc acgcccatca acaggaagaa aaaggggtag gtgatcagat
     9421 cgacgatggc cttggaccgt tcggagaaaa gggcgtaaag gacgtcagat ttgatgtgtt
     9481 cccggtgcag cagcacgtac cccccgccta tgatggcata ggcgccgaaa atgtggcccg
     9541 agatctcgtg ggcccagata gtgggcgcgt taaagacgta ccgcgaaata acctcgtaac
     9601 acaggatgcc gatcaagggc aggatcagaa aacaaaaggt ctttcccaag aaggtgttca
     9661 gtttcccgat aaaccggatg aaatacttca tcaacaaccc cccaatgcat tcgcaaagcg
     9721 ataggactca aaaggccgaa tttaaagggc gccctgggag cgcatcaaca gcccccgaat
     9781 gcatcatacc gccaatacaa acccgaagcc cgccctggtt cacggatacg gtcgaagcaa
     9841 gggcagggca taagttcaaa aggactggaa ctatgaagaa acctggcggc ggtccggcat
     9901 ttcggaaact cctccggcgc cgcctggaaa ccgtcggggc ataaaaaaac gtcactatcg
     9961 tcctgtcgaa gcgtcgtgat accgatcccg gttcatccga atgcgccgca cccggtgacg
    10021 accccaaatg gcggggaaca gagtaaacct gcaaggtccc ccagggcgtt tgactctttc
    10081 cccgatgaaa ggggaacctt gcaggcctat ttcggttgac aaaaatattt cgcccgtgcc
    10141 cgagccggtc atgaaaagca tcgtcgcctt ttggtccaag gcaccggcgg gatccctaac
    10201 gagaaacgtc ggggatcatg taacagttac ttcaaggagg gacttcggct tccttatcgg
    10261 ctgaacccga tcaggcctcc gtcacctcgg ccgggccata tccggcccgg cggccaggtt
    10321 attgttccat caaagctttt gccagctcag gcctgtattc ctggaggtag gccttaagca
    10381 cttcgatggc ttccttgctg gcggcgtcct tgccgaaatc gggtaggaag gaagcaaccg
    10441 tggcgttcca cttcagggcg tcctcttcgg accagtccac gatagtaacg cccagatcct
    10501 tggcctggcc ccaggctttg ccgttgtcgg ccgatccctg gaactcggcg aaggcgttgc
    10561 ccgccgcaag ggcggactcg acgacggcct tgagatcgtc gggaagctct ctccagactt
    10621 tgccgttgac gatgaagtag tcaacattga ccgggcccat caggggcgtc cggagccagt
    10681 acttggcgac ctcgtggaag cccatggcga tgctgtcggc gggaccgcaa taggtacagg
    10741 catcgataac tcccgatgag aggtttgtgt agatctcctc cccgggcgac caggtgacgg
    10801 aggctccgag agcaccgagg gcgttggcca cggggacgtc gccgctgcgg aacttcatgc
    10861 ccttgatctt atcgatgctg tcgatgggca ccttggagtt catgatcatt tcgagcacat
    10921 agtaggcagg gccgactaca tggacatcgc cgtatttggc cagttcttcc cgaaggaggg
    10981 ccaggatccg cccgtcgttc tgcttctcta catactggtg gagctcccag gtgttgttaa
    11041 gcaggaagag ggggttgg
//
LOCUS       DAOSRG010000025        10811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_5963_length_10811_cov_11.750465, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000025 DAOSRG010000000
VERSION     DAOSRG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5963_length_10811_cov_11.750465"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLM30_01920"
     CDS             <1..639
                     /locus_tag="PLM30_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOR53959.1"
                     /translation="LQRVFLEDWYVETLELIDERSGEPLFSHDGEHIFQAVPSGPSYR
                     TENYQRLVVSALHSASKEVVITTPYLIPDESLVEGLEVAVLNGADIKLVIPRRSDQFL
                     VGKAAMAYYDLLMDKGVRIFLYGDGVIHAKTMSVDGNLCFFGSSNFDIRSFTLNFEIN
                     CVIYGENSVAPILAEQKKFLEASTELSRPEWEKRPFLFKAVEGVAKLMSPLL"
     gene            646..1641
                     /locus_tag="PLM30_01925"
     CDS             646..1641
                     /locus_tag="PLM30_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53960.1"
                     /translation="MKTMGMERVLLLFDESRPFWTEELIELAGEDPAAAVSLEKEGYL
                     EDLEGGFCLTARGREAFLRWAAESFLESLPGEEPGDPVMEGLKLKTALLFERGFKGFQ
                     GTKKVTLSPGLEYFPRVPTGEAFSLSGGAIAWGLLEHPGVVKMVSSFPRGRDAGGESL
                     PELEGRVNGLGLERVPWSPHVLCLNQCDYAYYWRSRAETDRWGLLNTDRLFLNIVRDP
                     QSFGLGEAALDMAAFSLFLLDNRHVYLPGCFDIDIQQQSSFSWWFWATRTEEEARELA
                     LRLAIPGRDLVTPAEPTDAWVISLEALGASGHKAASFYEFVDDLALPVTRKGPGV"
     gene            1638..2489
                     /gene="zupT"
                     /locus_tag="PLM30_01930"
     CDS             1638..2489
                     /gene="zupT"
                     /locus_tag="PLM30_01930"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOR53961.1"
                     /translation="MNGLFFAFGLTLLAGMATGIGSAIAFFADRTNFRFLSVSTGFSA
                     GVMLYVSFVEIFMEGGRSLGTVFGARGEWMNTAFFFAGMALIGIIDYLIPEEENPHEA
                     RTQEETSHLHRDCPRGEVAPPLGEAGEKKLMRTGVFTALAIAIHNFPEGLVTFLAALQ
                     NPNLGIVIAVAVALHNIPEGISVSVPIFYATGDRTKAFFYSLLSGMAEPVGALVAYGL
                     IRLVLGGGEFSPAVTGALFTTVAGIMVYISLDELLPLSRAYGKGHDSLIGLVAGMAVM
                     ALSLLMM"
     gene            2520..3401
                     /locus_tag="PLM30_01935"
     CDS             2520..3401
                     /locus_tag="PLM30_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR53962.1"
                     /translation="MTPPSPGRLAPAVVLALGVIGISTGSIFARFADAPPLVISFYRT
                     GIATAAMAPLVLLRCREELFSLRGKDLLRILLSGAFLSLHFSTWITSLFYTSIASSVV
                     IVQTIPVWTAILSPFVSGDRVSGTTWAGILLSFIGVVVIGAGDFALGGEALFGDLLAL
                     LGAWFATGYFLTGRVVRARVSLPVYSFLCYGAACLILLAVIAPLGLPLRGFSGGTWGA
                     FLGMALVSQIMGHSSYNWTLKYLSATVVAVALLGEPIGATLLARLFFGETLTAAKVAG
                     GALILAGIVVAARGERT"
     gene            complement(3605..4168)
                     /locus_tag="PLM30_01940"
                     /pseudo
     CDS             complement(3605..4168)
                     /locus_tag="PLM30_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            4351..7416
                     /locus_tag="PLM30_01945"
     CDS             4351..7416
                     /locus_tag="PLM30_01945"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR53963.1"
                     /translation="MNAKKSLEPGHDPGIEPEEIKLTDSVDLAQIQSLIEVLSQTMGT
                     AAGLLDLEGNILAASGWQRIFTDFHEVHPQAHKACIESFARLSSGLERGKPILYKCWN
                     NLWDIATPVFVEGRRVGNLFIGQCFLEDEEPDIEVFKNQARKYGFDEKEYLDALAEVP
                     RISREKAEWAMALFVKIADHVSELGARNLQLARALEMNKTLLESLAASESLMRIAGDL
                     VALGGWQYYVGEDRFSYSEKVAEIHEVPIDFEPTLEEVIEFCAPEYRRRIRKVFKNCL
                     KKGDPFDEEVLIVTGSGRRVWVRTVGTPIRDEAGRVTRIHGAFQDISGRKKAEEALRQ
                     GASQFRAFVEGAPDGIFVQLNGRIAFFNETAGRLLKAGSVQELLGRKVVEFFSPGQTE
                     TIRRRLKRLNEEKKSVPPLETLLQCEDGSEVPVEISAVPFIYNGEAGSLAFIRDITRR
                     KKTEGALRQSEERFRSIFENATIGIYRTTPGGRVLMANPALVAMLGYSSFEELASRNL
                     NEEGFEPGYARSHFQDRIDRDGMIRGLESAWTRRDGFVVYVRESARAVRDDNGDVLYY
                     EGTVEDITAQKLAEEGLRRKNDLIRMAGELASLGGWSVSLPGGRIIWTDEAARIHEQP
                     PGFSATLEEVMSFYAPESRAQIEKVYEECIENGTPYDEEFRKITATGRSIWVREIGEA
                     VRDDTGKIIGAQGAFQDITERRRLESDYATLFREMLDGFSLNELICDADGKPIDFRFI
                     AVNPAFERITGLKPEEIKGKTILEVLPGIEDRWIQRFGRVVLTGEPTSFEDYVAPLGK
                     YFEVKVFRTARGEFVVISADITERKRTEEKLRHALETTIGVLTQTVERRDPYTAGHQR
                     RVGILAGRIAQEMGLDPEEVDQIRMSGFVHDLGKISVPAEILSKPGRLSELEMDLIRE
                     HPGHGRDILQDVESNWDLAKIVYQHHERLDGSGYPQGLKGDEISLEARILAVADVVEA
                     MASYRPYRPAIGMEEALEEIENNAGILYDPEVVKICLDLFRKKGYRLED"
     gene            complement(7436..9130)
                     /locus_tag="PLM30_01950"
     CDS             complement(7436..9130)
                     /locus_tag="PLM30_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR53964.1"
                     /translation="MKSKKVLIIGGVACGGKAAARLMRVDPEADVTVLEKGEYISYAG
                     CGLPYYVGGVVKEYRDLMTTPIGVVRDENFFRKVKHVTVHTQHLATKIDRRNKTVTAL
                     DLRNGEEKEFPYDKLVLSTGASPLRPPIPGSDLPRIFTLWNMPDALAMRAAIDSGKVR
                     NAVVIGAGLIGMEVVEALHDQGIQVSIVDLCETPLPQMMDREFGNLLRAVLEKKGINF
                     FGGEKVLEITGCNGEIKAVKTDKREVPADMVLMAVGVRPNVGIAREAGLALGSCGGII
                     VDDHMRTSDPDIYAGGDCVEVKNVLTGKPVWQPMGSVANRQGRVIADNIAGLPTTFKG
                     VAGTAIMKAFEYTIGKTGLTREQARLEGFDAEAVTIVDPDTPHFMPGSAAIQVRIVAD
                     RKTRKVLGAQVFGAGRADKRLDILVSAAAGGLTIDELADADIAYAPPYSTALDPVTHA
                     ANTLKNKIDGLMVSWSPSELTEKIKKGENPVLLDVRSPDEVKTQGTLPYETTLIPLGQ
                     LWDRGAELPRDREIIAFCKISVRGWDANSILKRLGYKKVSVLEGGIAGWPFELKKG"
     gene            complement(9259..10155)
                     /locus_tag="PLM30_01955"
     CDS             complement(9259..10155)
                     /locus_tag="PLM30_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR53965.1"
                     /translation="MTRIGLALGGGGARGAAHFGVLKVLEEERIPIDLIAGTSMGAIV
                     GAMYAQNPDASAVIERYERFIEDMDYDSFGMKYIAADADQDSHLLQKFARTIAQRIYM
                     SSMGRRPGIMKPEFLDKALKELLDRGDIQDTKIPFGAVATDLNSGSTILIREGSIRKA
                     VRRSALIPGYLPPEEDGKRLITDGGVSEPVPVDSALKMGADVVIAVSVDPATMSSLED
                     PSMINIMRRCDLIRGIYMSRVQIEKADVCLCPDMSDSDWSEFLSSGEFIKAGEEEARK
                     KLPQVRKALRKKRPWFLRFFSG"
     gene            complement(10317..>10811)
                     /locus_tag="PLM30_01960"
     CDS             complement(10317..>10811)
                     /locus_tag="PLM30_01960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR53966.1"
                     /translation="SRGHTVANHSWSHRNITQLDHETLFQELIYCSQAIEAITGIRPR
                     FFRPPGGKWDGQALAVVQELGLTTVLWDVTGRDMVQRSPGRIAASVVKATRPGSIILL
                     HGGMEWTMEALPVIVEGLRARGFLLVSLDQMFPFAGAARVPLRNPLAEATSPPAVHPV
                     KVVP"
BASE COUNT         1992 a         3288 c         3407 g         2124 t
ORIGIN      
        1 ctgcagaggg tcttcctgga ggactggtac gtggagaccc tggagctgat cgacgagcgc
       61 tccggcgagc cgctcttcag ccacgacggc gaacacatct tccaggcggt gcccagcggt
      121 ccctcctacc gtaccgagaa ctaccagcgg ctggtggtct cggcgctgca ctcggcctcg
      181 aaggaggtcg tcatcaccac cccctacctc atccccgacg agagcctcgt ggaggggctg
      241 gaggtggccg tcctcaacgg cgccgacatc aagctggtca tacccaggag gtccgaccag
      301 ttcctggtgg ggaaggcggc catggcctac tacgatctcc tcatggacaa gggcgtcagg
      361 atcttcctct acggtgacgg cgtcatccac gccaagacca tgagcgtcga cggcaacctg
      421 tgcttcttcg ggtccagcaa cttcgacata aggtccttta cgctcaattt cgagatcaac
      481 tgcgtgatat acggcgaaaa ctccgtggcc cccatactgg cggagcagaa gaagttcctc
      541 gaggcctcga cggaattgtc caggccggaa tgggagaaac gccccttcct cttcaaggcc
      601 gtcgagggcg tggccaaact gatgagccct ctcctctaga agggagtgaa gaccatgggc
      661 atggaacggg tgctgcttct cttcgacgag agcaggcctt tctggaccga agaattgata
      721 gagctggccg gggaagaccc cgccgcggcg gtttccctgg aaaaggaggg gtacctggag
      781 gacctcgagg gaggtttctg cctgacggca cgggggcggg aggcgttcct ccgctgggcc
      841 gcggaatcct tcctggaatc cctcccgggg gaagagcccg gtgatccggt tatggagggc
      901 ctgaagctga agacggcgct actctttgaa agaggcttca aaggcttcca gggcaccaag
      961 aaagtgaccc tgtcccccgg gcttgaatac ttccctcgtg tcccgacagg ggaggccttt
     1021 tctctctccg ggggggccat cgcctggggc ctcctcgaac accccggggt ggtgaaaatg
     1081 gtatcttcct tcccccgggg tcgcgatgca ggaggggaaa gcctgcccga actggaggga
     1141 agggtgaacg gcctgggcct tgaaagggtc ccctggtcac cccatgtgct ctgcctcaat
     1201 cagtgcgact acgcctacta ctggcgaagc cgggccgaga cggaccggtg ggggcttctg
     1261 aacacggacc gcctcttcct gaacatcgtc cgggaccccc agtccttcgg gctgggtgag
     1321 gccgccttgg atatggccgc cttctccctc ttcctcctcg ataacaggca tgtctacctc
     1381 ccggggtgct tcgacatcga catccagcag cagagcagtt tttcctggtg gttctgggcc
     1441 acccgtacgg aggaggaggc cagggaactg gctctacgat tggcgatccc gggaagggac
     1501 ctggtgaccc cggcggaacc gaccgatgcc tgggtcataa gcctggaggc cttgggtgcc
     1561 agtggtcaca aagccgcgtc cttttacgag ttcgtcgacg acctggcctt accggtgacc
     1621 aggaagggac cgggagtatg aacggccttt tcttcgcctt tgggctgacc ctcctcgccg
     1681 gtatggcgac cggcatcggc agcgccatcg cctttttcgc cgacaggaca aacttccggt
     1741 ttctatcagt ttccaccggc ttctcggcgg gagtgatgct ctacgtgtcc ttcgtggaga
     1801 tattcatgga agggggccgg tcgctgggaa cggttttcgg ggccaggggg gaatggatga
     1861 acacggcctt ctttttcgcc ggaatggctt tgataggtat tattgactac ctgatcccgg
     1921 aagaggaaaa cccccacgag gcgaggaccc aggaggaaac ctcccacctt cacagggact
     1981 gccccagggg tgaggtcgcg cctcccctgg gagaggccgg ggagaagaag ctgatgcgga
     2041 cgggagtctt caccgccctc gccatagcca tccacaactt cccggaaggg ttggtcacct
     2101 ttcttgccgc attgcagaac ccgaacctgg gtatagtcat cgccgtggct gtggcgcttc
     2161 acaacatccc cgaggggatc tccgtctccg tgccaatttt ttatgccacg ggagacagga
     2221 ccaaggcttt cttttactcc ctgctcagcg gcatggccga gcccgtaggg gccctcgtgg
     2281 cctacggcct gatccgcctc gtcctgggcg gcggagaatt ctcaccggcc gtcaccggag
     2341 cccttttcac cacggtggcc ggcataatgg tctacatcag ccttgacgaa ctcctccccc
     2401 tgagccgtgc ctacggcaag gggcacgaca gcctgatcgg cctggtggcc gggatggccg
     2461 tcatggccct cagcctcctg atgatgtaag cactcccaat gaaaggtgga agctttcgaa
     2521 tgacaccccc ttcaccggga cgcctggcgc ccgccgtagt tcttgccctt ggcgtcatcg
     2581 ggatatccac aggttccata ttcgcccgct tcgccgacgc gccacccctg gtgatctcct
     2641 tctaccgaac gggtatcgcg acggcggcca tggcgccgct ggtgctcctg cgctgccgcg
     2701 aggagctttt cagccttcgc ggaaaggacc tcctgcggat tcttttgtcg ggcgcgttcc
     2761 tgtcgttgca cttttccacc tggatcacgt cgctttttta cacctccatc gccagcagcg
     2821 tggtcatcgt ccaaacgatc cccgtgtgga cggctatcct ctcgcccttc gtgagcggcg
     2881 acagggtgag cggtacgacc tgggcgggca tcctgctcag cttcatcggc gtcgtcgtga
     2941 taggcgccgg ggacttcgcc ctggggggcg aggccctttt cggcgacctt ctggcgctcc
     3001 tgggggcatg gttcgccacg ggctacttcc tcaccgggag ggtggtcagg gccagggtca
     3061 gcctccccgt ctattccttc ctctgctacg gggcggcctg cctgatcctc ctggccgtca
     3121 tcgccccttt gggccttcct ctgcggggct tcagcggcgg cacctggggc gccttcctcg
     3181 gcatggccct ggtgtcgcag ataatgggtc acagcagcta caactggacg ctgaagtacc
     3241 ttagcgcgac cgtcgtggcc gtggcgctcc tgggagaacc catcggcgcc accctgctgg
     3301 cccggctgtt cttcggggag accctgacgg cggccaaggt cgccggcggc gcgctcatcc
     3361 tcgccgggat cgtcgtcgcc gccagggggg agaggacctg agagaggcgg acgaaggtga
     3421 accctttctt cttcagcgcg ccgagagcgg cggcgaggcc ctcggcggtc tgcccttcgg
     3481 gtcggttcat gtgcatcagg actatatcgc cggggagggc cgacatcagt gccttcgcca
     3541 cccggtccgc ggagtaggtg gcaccggcgt cgccgttcac gctgcagccc acgacccggt
     3601 ggcccagttc tccggcgatc ctcacggcga cctcgtcgta gtggttggtg ccggagcgga
     3661 agaagagcgg ccttttgccc gacgcggtct cgatcgcccg ggcaccttcc tcgacttccc
     3721 gggcggcttc cgccgggctt cccgtccccc ttatgccgta aacctccctc ccgctcaccg
     3781 aaagaggttt gtggtccagc ccgtggttgg cgatgtcgaa aagagggtcc gtctcgaggg
     3841 aggcgaaggt ctcggggttc gcccttatcc agcgggcgtt tatgaagagg gtggcgggta
     3901 tgccctcctt ccggaggagt tcgatgagtt cctcgtcgta accgcagccc ttgacccccc
     3961 cgccgcaggc gtcgaaggta agggccagcc ctttgccccc gccggggttg aagcgggtta
     4021 cgacgccggt gacctcctgt ccccactcga gggggacgac gccggcgtat cccccgatca
     4081 cttcctgccg attcccgacc gcccccgcgg gggagggctg aagcgccggc cggcaaaaca
     4141 ggcaaaaaat agcgacccag gagagcattt tcctgatgac gaccatcccc ttccttcgcc
     4201 ttcattttac aacttcctcc catggccccg tttttttgaa gcgggggggg ctgaagtacg
     4261 gagccgccgg tccctggtga ggttgttttt tccgggtcta aggatgtaat ataaacagaa
     4321 gtttgaaatt attcccaaga gggaaaggtc atgaacgcga aaaagagcct cgaaccgggg
     4381 catgatccgg ggatcgaacc cgaagagata aaactcaccg acagcgtcga cctggcccaa
     4441 atccagagcc tcatcgaggt gctatcccag acaatgggga cggctgcggg gctgttggat
     4501 ctggaaggca atatcctggc ggcctcgggc tggcaaagga tcttcaccga cttccacgag
     4561 gttcatcccc aggcgcataa ggcctgcatc gaaagcttcg cccgcctctc gtcgggcctg
     4621 gagcggggca agcccattct ttacaagtgc tggaacaacc tctgggatat cgctaccccc
     4681 gttttcgtcg aaggccgcag ggtgggaaac ctcttcatcg gccagtgctt cttggaagat
     4741 gaagaaccgg atattgaagt cttcaagaac caggccagaa aatacgggtt cgatgaaaag
     4801 gagtatctcg atgccttggc ggaggtcccg aggatatccc gggagaaggc cgaatgggcc
     4861 atggcccttt tcgtcaagat cgccgaccac gtctccgaac tgggggccag gaacctccag
     4921 ctggctcggg ccctggaaat gaacaaaacc ctgctggaga gcctggccgc cagcgaatcc
     4981 cttatgagga tcgccgggga cttggtggcc ctcgggggat ggcagtacta cgtcggggag
     5041 gaccgtttct cctactcgga aaaggtggcc gagatccatg aagtgccgat cgactttgaa
     5101 cccacccttg aagaagtcat tgagttctgc gcgcccgagt accggcggag gatccggaag
     5161 gttttcaaga actgcctcaa gaagggcgac cccttcgacg aggaggtgct gatcgtcacc
     5221 gggagcggcc gccgggtctg ggtaaggacc gtggggacgc cgatccgtga cgaggcgggc
     5281 agggttaccc gcatccacgg cgccttccag gatataagcg gccgaaagaa ggccgaggag
     5341 gccctgaggc agggcgcgtc acaatttcgg gcctttgtcg agggcgcgcc cgacggcata
     5401 ttcgtccagt tgaacggaag gatcgctttt ttcaatgaga cggccggccg cctgctgaag
     5461 gccggttcgg tccaggaact gctggggcgg aaggtggtgg agtttttctc ccccggccag
     5521 accgaaacga tccgccggcg gctgaaaagg ctgaacgagg agaaaaaatc cgtccctccg
     5581 ctggagaccc tgctgcaatg cgaggacggc tcggaagtcc ccgtcgaaat atcggccgtg
     5641 ccttttatct acaacggcga agcgggttcc ctcgccttta tccgcgacat aacccgacgg
     5701 aaaaagaccg agggggccct gaggcagagc gaggaacgct tcaggagcat cttcgagaac
     5761 gcgaccatcg gcatctacag gaccaccccc ggcggcaggg tcctcatggc gaaccctgcg
     5821 ttggtggcca tgctcggcta ttcctctttc gaggaactgg cctcgaggaa cctcaacgag
     5881 gaaggttttg aaccagggta cgccaggagc catttccagg ataggattga ccgggacggg
     5941 atgataaggg gcctcgagtc ggcctggacc aggcgggacg gcttcgtggt ctatgtcagg
     6001 gaaagcgcca gggcggtcag ggacgacaac ggggacgtgc tttactacga ggggaccgtg
     6061 gaggatatca ccgcccagaa gctggccgag gaagggttga gaaggaagaa cgacctcatc
     6121 cggatggccg gcgaactggc ttccctgggc ggttggagcg tgagcctgcc cggtggccgc
     6181 atcatctgga ccgacgaggc cgccaggatc catgagcagc ctcccggttt ttcggctact
     6241 ttggaagagg tcatgagttt ttacgctccc gaatcgcggg cccaaataga aaaggtttac
     6301 gaagagtgca tcgaaaacgg gacgccctac gacgaggagt tcaggaagat caccgccacc
     6361 ggtcggagca tctgggtgcg ggaaataggg gaggccgtga gggatgatac cgggaagatc
     6421 atcggggccc aaggtgcctt ccaggatatt acggagcgcc gcagactcga gagcgactac
     6481 gctaccctct tccgcgaaat gctcgatggc ttttccctca atgagttgat ctgcgatgct
     6541 gacggcaagc ccatagactt ccgttttatc gccgtcaacc ccgccttcga gaggataacc
     6601 gggctcaaac ccgaagagat caagggaaaa accatcctcg aggtcctccc aggcatcgag
     6661 gatcgctgga tccagaggtt cggccgggtg gtgctgacag gggagcccac ctccttcgag
     6721 gactacgtcg cgcccctggg gaaatacttc gaagtgaagg tctttcgcac ggcccgcggg
     6781 gaatttgtcg ttatatcggc cgatatcacc gagaggaaac gcactgaaga aaagcttcgg
     6841 cacgccctgg aaaccaccat aggggtcctc acccagaccg ttgagcgcag ggacccctac
     6901 accgcaggcc accagaggcg agtaggcatc ctggccggaa ggatagccca ggaaatggga
     6961 ctggacccgg aagaggtgga tcagatcagg atgtcaggct ttgtccatga cctggggaag
     7021 atatccgtcc cggcggagat cctgagcaaa cccggcaggc tttccgaact ggagatggac
     7081 cttatcaggg agcaccccgg ccatgggagg gatatcctcc aggacgtgga aagcaactgg
     7141 gacctggcca agatcgtata ccagcatcac gagcgcctcg atggttccgg gtacccccag
     7201 ggtctgaagg gcgacgagat aagcctcgag gccaggatac tggccgtcgc cgacgtagtg
     7261 gaggccatgg cctcctaccg gccctaccgg cccgccatcg ggatggagga agccctggag
     7321 gagatcgaga acaatgccgg tatcctctac gaccccgaag tggtgaagat atgcctggac
     7381 ctcttcagaa agaaggggta ccggctggag gattgaccag caggcccccc ttcttttatc
     7441 cctttttcag ttcgaagggc cagcccgcaa tgccgccctc caggacggat acctttttgt
     7501 aacccagcct cttgagtatg gaattggcat cccatccgcg caccgatatc ttgcagaagg
     7561 cgatgatctc cctgtccctg gggagttccg ccccccggtc ccacaactgc cccagcggga
     7621 tgagggtggt ctcgtaggga agggtaccct gggtcttcac ctcgtcggga gagcggacgt
     7681 cgagaagcac ggggttctcg ccctttttga tcttctcggt cagttccgag gggctccagg
     7741 agaccatcag gccgtctatc ttgttcttca gggtgttggc cgcatgggtg acggggtcca
     7801 gcgccgtcga gtagggggga gcgtaggcga tgtcggcgtc ggccagttcg tcgatggtga
     7861 ggccccccgc cgcggccgag acgaggatgt cgagcctctt gtcggccctg ccggcgccga
     7921 aaacctgcgc gcccaggacc ttgcgggtct tcctgtcggc gacgatacgg acctggatag
     7981 cggcggaacc gggcatgaag tgaggggtgt cgggatcgac gatggtcacg gcctcggcat
     8041 cgaaaccctc cagccgggct tgttcccttg tcaggcctgt cttccctatg gtgtactcga
     8101 aggccttcat gatggccgtc cccgccacgc ccttgaaggt ggtcggcagc ccggcgatat
     8161 tgtcggctat tacgcgaccc tggcggttcg ccacggaacc catgggttgc cagacgggtt
     8221 tgccggtgag gacgtttttc acctcaacgc agtcgccgcc ggcgtagatg tcggggtcgc
     8281 tggtgcgcat gtgatcatcg acgatgatcc ctccacagct gcccagggcc aggccggcct
     8341 ccctggcgat ccccacgttg ggcctgacgc ccacggccat gaggaccatg tcggcgggga
     8401 cctcccgttt gtcggtcttc acggccttta tctcgccgtt gcagccggtg atctccagga
     8461 ccttttcgcc gccgaagaag ttgatgccct tcttctcgag aacagccctg agaagattgc
     8521 cgaattcccg gtccatcatc tggggcaggg gcgtctcgca gaggtcgacg atggacacct
     8581 ggataccctg gtcatggagg gcttccacga cctccatccc gatgaggccc gcccctatga
     8641 ccaccgcgtt cctgaccttg ccgctgtcga tggcggcgcg catggcgagc gcgtcgggca
     8701 tgttccagag ggtgaagatc ctgggcagat cggaaccggg aatagggggt ctcagggggc
     8761 tggcgccggt ggacaggacg agtttgtcgt aggggaactc cttctcttcg ccgttacgca
     8821 ggtccagggc cgtgacggtc ttgttccgcc tgtcgatctt cgtggccagg tgctgggtgt
     8881 gtacggtgac atgcttgacc ttccggaaga agttctcgtc cctgaccact cctatggggg
     8941 tggtcatgag gtcccggtat tccttgacga ccccgccgac atagtagggg aggccgcacc
     9001 ccgcgtaact gatgtattcg cctttttcaa gcacggtgac gtcggcttcg gggtcgaccc
     9061 tcatgagcct ggccgccgcc ttgcctccac aggctacacc gccgatgatg agcacctttt
     9121 tgctcttcat gatccatccc tcccagggga caagatttct ttcactcctt ggcagaagaa
     9181 actgcctgaa acctcgggat ctggcgcaag gttattatac caagggggag tatgtcaaga
     9241 cggaatgaag gcaaaggatc agcccgaaaa gaaacggaga aaccagggtc ttttcttgcg
     9301 cagggctttt cgtacctggg ggagtttttt cctggcctct tcctcccctg cctttatgaa
     9361 ctcgccggag gagaggaatt cgctccagtc gctgtcgctc atgtcggggc agaggcatac
     9421 gtcggctttt tcgatctgca ctctcgacat gtagatcccc cggatgagat cgcagcggcg
     9481 catgatattt atcatgctgg ggtcctcgag gctggacatg gtggcggggt ccaccgaaac
     9541 ggctatcacc acgtcggcgc ccatcttcag ggcggaatcc acgggcacgg gctcgctcac
     9601 tccgccgtcg gtgatgagtc tcttgccatc ctcctcaggg ggaagatagc ccgggatcag
     9661 ggcgctcctc cggacagcct tcctgatgct tccctcccgg atcaggatcg tgctcccgga
     9721 gttgaggtcc gtcgccacgg cgccaaaggg gatcttcgta tcctggatgt cgccgcgatc
     9781 cagtagttct ttcagggcct tgtccaggaa ttcgggtttc attatccccg gccgtctacc
     9841 catggagctc atgtagatcc tctgggctat ggtcctggcg aacttctgca gcaggtggga
     9901 atcctggtcg gcatcggcgg ctatgtactt catgccgaag gagtcgtaat ccatgtcctc
     9961 gatgaagcgt tcgtaccttt cgatcaccgc cgaggcatcg gggttctggg catacatggc
    10021 gcccacgatg gctcccatgg acgttccggc tatcaggtct atggggatcc tttcctcttc
    10081 cagcaccttg aggaccccga agtgggcggc tcccctggcg cctcctcctc ccagggccaa
    10141 cccgatcctg gtcaaagcag ccacctcccc gcttccatta tatccccctt cgctggaagg
    10201 ggcgactcca cgacggacct tatggtaatg cctgcgaaag ggggatggcg gggggggttt
    10261 agggctagtg tcgaggcaag ccttcaccgg cgacggaaat ggcttcccat gggcgttcag
    10321 ggtacgacct tcacggggtg cacggctggt ggcgacgtcg cctcggcgag ggggttcctg
    10381 agggggaccc tcgcggcccc ggcaaagggg aacatctgat cgagggagac caggaggaac
    10441 cctcttgccc tcagcccttc cactatcacc ggcagcgcct ccatggtcca ctccatccct
    10501 ccgtggagga gtatgatcga cccgggccgg gtggccttga cgaccgaggc cgctatccgg
    10561 ccaggcgacc tctggaccat gtccctgccg gtcacgtccc agagcaccgt ggtgaggccc
    10621 agttcctgga cgaccgccag ggcttgccca tcccatttgc ctcccggcgg ccggaagaac
    10681 cggggtcgta tccccgtgat ggcctcgatg gcctggctgc agtagataag ttcctggaaa
    10741 agggtctcat ggtccaattg ggtgatgttc ctgtgggacc aggagtgatt tgccacggtg
    10801 tgtcccctcg a
//
LOCUS       DAOSRG010000026        10723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6021_length_10723_cov_7.595894, whole genome shotgun sequence.
ACCESSION   DAOSRG010000026 DAOSRG010000000
VERSION     DAOSRG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_10723_cov_7.595894"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLM30_01965"
     CDS             complement(<1..359)
                     /locus_tag="PLM30_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR53967.1"
                     /translation="MISLWETTRISSRALAVNKMRSALTMLGIIIGVMAVIMMFAIGA
                     GANREISDRFSSMGSNLLTVRPGAVQAPGGVRSGSVNTLTVDDARAIAEECAAVAYAA
                     PVYGGGAQVVYGNQNWST"
     gene            complement(356..1057)
                     /locus_tag="PLM30_01970"
     CDS             complement(356..1057)
                     /locus_tag="PLM30_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR53968.1"
                     /translation="MATDGIVHLEGITKIYDLGDVQVEALRDVSFSVAEGEFLSIMGA
                     SGSGKSTLMNILGCLDTPTRGRYYLQGFDVSGLSRDELADIRKHKIGFVFQGFNLLPR
                     MTALENVQLPMLYNGVPPSLREGKAMEALEMVGLGDRAHHQPQQLSGGQQQRVAIARA
                     LVNDASIILADEPTGNLDSRTGRDIMGLFTELNEKLGRTIIQVTHEAEMAAYGKRTIT
                     FRDGMILKDERTGGT"
     gene            complement(1065..2246)
                     /locus_tag="PLM30_01975"
     CDS             complement(1065..2246)
                     /locus_tag="PLM30_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR53969.1"
                     /translation="MMKVMLKGRKFLVAGVLLLAAAGFLFYHGRSSGLPEVQTTAVLR
                     GSIVKTVSATGSLNAVNTVNVGAQVSGTIKKIHVDYNSVVTRGQLLAEIDPSLLEANI
                     RQSEASVLSASASLQEVRANLAEAKKNLERNRDLFSRGYIAAMELDQSQTAYETSLSR
                     LKSAEASLSQARADLEYKKINLGHASIVSPIDGVVIDREVDEGQTVNASQSAPTLFTI
                     AENLARMQVEANIDEADIGQVRRGQTVQFTVDAFPEESFTGVVKEVRLAAATTDNVVT
                     YRVVISVDNERKILMPGMTANVSVIVEKKGDILKVASSALRFKPTGESGSSRPKGAVL
                     WTLEKGELVPHPVKTGIYDGMYTEVSGEGVTEGMVVVTYMSAATSGRASSAFGFGGRP
                     R"
     gene            complement(2243..3529)
                     /locus_tag="PLM30_01980"
     CDS             complement(2243..3529)
                     /locus_tag="PLM30_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR53970.1"
                     /translation="MALTKKAFPLVLLLLLLSVPASAANEAAPVMMSLDECIERALAG
                     HPDLAAGRADMAASEARAGQIRAGYSPSMSFSSGFSEKSSSGPGPSEGSWSSGITLSQ
                     VVSDWGRTHISLRRSLLDAEGKALALKAKRSDIVFEVTRSYYQVLKSMKNLEVAEESL
                     ALNEERLGQAEAFFEVGRVSKYDVTAARVSRSNANLAHVQAGTALKEAMTSLKAAIGS
                     PGMPDFSLAEVADGQGSPPPADLPSLEAALGYALGNRPDLKIYGVNLASAEAAAALAG
                     LDNAPKLDLSAGYGWGSTDFWGQDSWRAGLTLSFPLFDGGLRREKIREAAANVEGAEA
                     RLESYRQQVLKDVTNAWLAASDSAESEAAAAEGLKMASENLEIASGRYRTGVGSPLEV
                     SDATKNFAEAKAAWYGALYDGMTARAALEKAMGVER"
     gene            complement(3724..4266)
                     /locus_tag="PLM30_01985"
     CDS             complement(3724..4266)
                     /locus_tag="PLM30_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOR53971.1"
                     /translation="MNEDAAAGVTRILAEAGFHDHQWVRPSELVISQWVRVKCMFGCP
                     DYGRNASCPPHVPSVPECRELLREYSRALLIHLPCKVPPEGRRQWCLENQERLVEVER
                     RVFLSGFEKAFLLSFDNCNLCDECVAEPSDCRFPYKRRPTMEAFAIDVYSSVKKYGYT
                     LSVKKDRLEEPDRFALLLVE"
     gene            4422..5402
                     /locus_tag="PLM30_01990"
     CDS             4422..5402
                     /locus_tag="PLM30_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOR53972.1"
                     /translation="MSRSKRGFTLVEILLTILIIAILAGNLLLTSGISRDRSAATRIV
                     SDLMTLKRTAMLFFSDNDRWPGESDYERLNAYLSGHIQLQAWDEDSGVYAIKRHEALG
                     TVYAAARVADEFGGSPRIREFLERDSSEYGLLNGELSPYQSVDPVVAVRVKDIGPGSD
                     EGGEDDHSGPEGPGGGAGQDSGSGTGGNHPDHDTWDSGRAYLGGERVVFDGGVFEARY
                     WTQGNQPGLTDSPWQEITDEWRDFNEYKQGDIVVYNGKQYQAWYWTKDNTPGVVGSPW
                     QQVGTDQWVASNKYAGGDFVYYDGKQYKAKWNVDPGKNPPGSSDAWELIP"
     gene            5428..5868
                     /locus_tag="PLM30_01995"
     CDS             5428..5868
                     /locus_tag="PLM30_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOR53973.1"
                     /translation="MATVTAVCAGEDRQKPKNALEAARFVEGMGIAGDGHFGMGDRQV
                     SLLRSEDLALAESEAGFPFPPGSLAENLVVRGLPTELAPGSLLRIGAEVVLEVVEKGK
                     RPDEPHSYDYRGWCLLPTAGYFLKVLTGGEVRPGDTVILEGKAP"
     gene            complement(5906..6766)
                     /gene="otsB"
                     /locus_tag="PLM30_02000"
     CDS             complement(5906..6766)
                     /gene="otsB"
                     /locus_tag="PLM30_02000"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOR53974.1"
                     /translation="MGETGRPSAKEGGSSGADPCRLPSALDSMGLIEEAARGRRIFAA
                     FDFDGTLTPIVKDPYEAYLDPAMRSRLETLSRVAAVAVVTGRDLGDIRRRVGIPGLFY
                     SGCHGFEIAGPSGSGVAFRTGEEYTHVLEEAGERFEKIVGGFPGAILQRKRYCLTVHY
                     RLVAEEDVGSLIGLIEEAAADVGGLRARPDKKAVEVLPDMDWNKGSAVLWLLDRLGMT
                     QAGSFALYVGDDITDEDGFRAVAGRGAGVIVAGAGSDRGTLATYRLDGVEGTALFIEG
                     LTRIASPGAP"
     gene            complement(6759..7100)
                     /locus_tag="PLM30_02005"
     CDS             complement(6759..7100)
                     /locus_tag="PLM30_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOR53975.1"
                     /translation="MNFGYRDGKVYMHCAPEGRKLDVIGKNPYVCLEFEADLQMKYAQ
                     EACDFTMYYKSVIAWGRAEVLREPEEKARGMNVIMGHYMGKEYEFPDQALARIVIIRV
                     DVEEMTGKQNG"
     gene            7881..8699
                     /locus_tag="PLM30_02010"
     CDS             7881..8699
                     /locus_tag="PLM30_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR53976.1"
                     /translation="MRCPVRVVFIAVLVSLFAFPAFPSDVPGSKDHPLLPRITGYYIG
                     DYQEVEFGSHDFIDAEGDYLTVEGRRIQIEYDINEGESPPGDLFVIVNYERAASGAGG
                     SFYRHRDDMTYLTLQQDSTETWAEVMSQGDGQTYWLVIVEKGVAEQRISANEMARALK
                     TAGRVSLSIHFDTGKAVIRTESQAIIDEIFQMMTEDGRMRLKVEGHTDSVGNEADNLA
                     LSKDRAMAVVQSLVEKGIEPERLEYEGFGELRPVSDNETASGRAKNRRVDLVLL"
     gene            8711..10585
                     /locus_tag="PLM30_02015"
     CDS             8711..10585
                     /locus_tag="PLM30_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53977.1"
                     /translation="MSNRAVERIALAVLLACAVIVGIHSGAQGALPDSIPVSIHLEAS
                     WNTDEGDSQLFETGSCSINVSGQMVRSAGDNIFFSHYIPSGVTASYRYDESIIARPSD
                     EESECRGEIGKVFASGSIDVGFEPGTDKASLFLQSFTGPLGVGAFLQATADPIILDPE
                     SLIERMANDPDRTVYSFFLMVPVTASVEEVSGCETLFTKTLDLALAANAVSEMGITGL
                     TGSYSWDAEDPPKSGASLFAVGEKIHYGPEPGTKKHGSVTFSWTFGEIEPVVALYWVK
                     ETERVEFTGEEIEVMAGRKVRLEAEVYPQADEASAGHWELPPSFIAGLEGYDRGNFAR
                     GKAILLSEEEKRSRRIDFYCLDGAFQGKDQKIAYAATIKGEKVEGKGTVKVFSPEADI
                     DVIPEKRVSIGGVSSGKGCSAYLGRIAPPLSIPGIEIKGKARFPAGFEKEEAEHSICF
                     VQLLREDILERKGRFGQQGGDYFQLVSENFSLDTRFPYGGLEGKESLEMNDVPSDEIG
                     PLTQNIHHFQDFRTTLLIRPGPGGKAIWLPIETIPWSWRFSLERVKDNPGWDETCLPG
                     NFRITNTRFVIPGKREKFSGKLEDVPSWDKVLDGSEKAMHHNDDNRWLDDVNTFRKIK
                     "
BASE COUNT         2174 a         3265 c         3152 g         2132 t
ORIGIN      
        1 gtggaccagt tctggttgcc gtagacgacc tgggcgccgc cgccgtagac gggggcggcg
       61 taggccacgg ctgcgcactc ctccgctatc gccctggcgt cgtccacggt gagggtgttg
      121 accgaaccgc tccgcacgcc gcccggcgcc tgtacggccc cgggtctcac cgtcagcagg
      181 ttgctcccca tgctggagaa ccggtcggag atctcccggt tggcgcccgc cccgatggcg
      241 aacatcatga tcaccgccat gacgccgatg attatcccca gcatcgtcag ggccgatcgc
      301 atcttgttga cggccagtgc cctggaggag atcctcgttg tttcccagag ggatatcatg
      361 tcccaccggt cctttcgtcc ttcaggatca ttccgtcccg gaaggtgatg gttcttttcc
      421 cgtaggcggc catttcggcc tcgtgagtga cctggattat ggtcctgccg agcttttcgt
      481 tcaattccgt gaagaggccc atgatatccc tgccggtcct gctgtccagg ttccccgtag
      541 gctcatcggc caggatgatc gaggcgtcat tcaccagggc gcgggcgatg gccaccctct
      601 gctgctggcc gcccgacagc tgctggggct ggtggtgagc cctgtcgccc agccccacca
      661 tctccagggc ctccatggcc ttcccctccc tcagggaggg gggaacgcca ttatagagca
      721 tgggcagctg gacgttctcc agggccgtca tgcgaggcag caggttgaaa ccctggaaga
      781 cgaaaccgat cttgtgcttg cggatgtccg ccagctcgtc cctcgacagg ccgctgacat
      841 caaagccctg caggtaataa cggcccctcg tcggtgtgtc gaggcacccc aggatgttca
      901 tcagcgtcga cttgcccgag ccggaagccc ccatgatgga aaggaactcc ccctcagcca
      961 cggaaaaact gacgtccctg agggcctcca cctggacatc gccgaggtcg tatattttcg
     1021 tgatgccctc caggtggact atcccgtccg tcgccatcag gccctcaccg gggtcggccc
     1081 ccgaacccga aggcgctgga tgctctcccc gacgtcgcgg cggacatgta agtcaccacc
     1141 accatgccct cggtgacgcc ctcgccggag acctcggtgt acatgccgtc gtagatgccg
     1201 gtcttcaccg ggtggggcac cagttccccc ttttcaaggg tccagaggac ggcccccttc
     1261 ggcctggagc tgccgctctc ccctgtgggc ttgaacctca gggccgaact ggccaccttc
     1321 aaaatatctc cttttttttc gacgatgacc gaaacgttgg ccgtcatgcc cggcatcagg
     1381 atcttcctct cgttgtccac tgaaatgacg accctgtagg tcaccacgtt gtcagtagta
     1441 gcggctgcga ggcggacctc tttgacgacg ccggtgaagg attcctcggg gaaggcgtcc
     1501 accgtgaatt ggacggtctg tccccgtcgg acctggccga tatcggcctc gtcgatgttg
     1561 gcttccacct gcatcctcgc caggttctca gctatggtga agagcgtcgg cgccgactgg
     1621 ctcgcattga ccgtctggcc ctcatccact tccctgtcga tcacgacacc gtcgatgggc
     1681 gacacgatgc tggcgtgccc gaggttgatc tttttgtact ccaggtccgc cctggcttgg
     1741 gagagggagg cctcggcgct cttcaggcgc gagagggagg tctcgtaggc cgtctgggac
     1801 tggtcgagtt ccatggccgc gatgtaaccc cttgaaaaga ggtcccggtt gcgctccagg
     1861 ttctttttcg cctcggccag gttcgcccgg acctcctgga gcgaagcgga ggcggagagc
     1921 acgctggcct cggactgcct gatgttggcc tcgagcagcg acggatctat ctccgcgagg
     1981 agctgccccc tggtcacgac ggagttgtag tccacgtgga tcttctttat cgtgcccgag
     2041 acctgggccc cgacgttcac cgtgttgacg gcgttcagcg accccgtggc ggaaacggtc
     2101 ttcacgatac tcccccgcag gacggcggtg gtctggacct cggggaggcc tgaagagcgg
     2161 ccgtggtaga acaggaagcc cgccgcggcc agtaaaagga cccccgcgac cagaaatttt
     2221 cgacctttca gcatgacctt catcatcgct ccactcccat cgccttctcg agggcggccc
     2281 tcgcggtcat cccgtcgtag agcgccccgt accacgcggc tttcgcctcg gcgaagttct
     2341 tcgtcgcgtc ggagacctcc aggggggagc cgacccccgt cctgtacctc cccgaggcga
     2401 tctccaggtt ctccgatgcc atcttcaggc cctcggcggc ggcggcctcg gactcggccg
     2461 aatcggacgc ggcgagccag gcgttcgtga catccttcag cacctgctgt cggtaggact
     2521 caagccgggc ttccgctccc tcgacgttgg ccgcggcctc ccggatcttc tctctcctca
     2581 ggccgccatc gaaaagagga aaggacaggg tcaaccccgc tctccagctg tcctgccccc
     2641 agaaatcggt cgaaccccag ccgtaacccg ccgacaggtc aagcttcggg gcgttgtcca
     2701 gcccggcaag ggcggcagcc gcttccgccg acgctaggtt caccccgtag atcttcaggt
     2761 ccggccggtt cccgagggcg tagcccaagg cggcctccag cgacggcaaa tcggccgggg
     2821 ggggcgatcc ctggccatca gcgacctccg ccagggagaa gtcgggcatc ccgggggaac
     2881 ctatcgccgc cttcagcgag gtcatcgctt ccttgagggc ggtcccggcc tgcacgtggg
     2941 ccaggttggc attgctcctt gagacccggg cggccgttac gtcatacttc gatacgcgtc
     3001 ccacctcgaa aaaggcctcg gcctgtccca gcctctcctc gttcaaggcc aggctctctt
     3061 ccgcgacctc gaggttcttc atgctcttca gaacctggta ataggacctc gtgacctcga
     3121 aaacgatgtc ggagcgctta gccttcagcg ccagggcctt cccctcggca tcgagcaggc
     3181 ttctcctaag ggagatatgg gtccgtcccc agtcgctcac gacctgcgat agggttatac
     3241 cgctcgacca cgaaccttcc gagggacccg ggccggagga ggacttctcg gaaaaacccg
     3301 aggagaagct cattgatggg ctatagcccg cccggatctg acctgccctg gcttccgaag
     3361 cggccatgtc cgcccgtccg gcggccaggt cagggtgacc ggcgagggcc ctctcgatgc
     3421 actcatcgag gctcatcatc accggggcgg cttcgttcgc cgcactcgcc gggaccgaga
     3481 gcaggagtaa cagtaaaacc agggggaacg cttttttcgt cagcgccatg gcttaccacc
     3541 tatctcgctt tgtttgccca caattttaat ctctcaatga tgaagacgga gtagagaaat
     3601 gtaaaggatg gttaagagac ttcgggccac cccggaagtg cttgaaggga tatcctcctt
     3661 ttcagacctt ccggcggttc cgaataggca caccttggtc cggggaaaga acgatgcctg
     3721 cctttactcg accagcagca gggcgaagcg gtccggttct tcgagacgat cctttttgac
     3781 cgaaagggta tacccgtact ttttcacgct ggagtaaaca tcgatggcga aggcctccat
     3841 ggtgggacgt cttttgtagg gaaatcggca gtcgctgggc tcggccacgc actcatcgca
     3901 gaggttgcag ttatcgaagg agaggaggaa ggccttctcg aaacccgaga ggaatacccg
     3961 cctctccacc tcgaccagcc tctcctggtt ctcgaggcac cactgccgcc tcccctcggg
     4021 cggcactttg cagggcaagt gtatcaggag agcccgggag tactcacgca gcagttcccg
     4081 gcactccggc accgatggga cgtgaggcgg gcaggaggcg ttcctgccgt agtcggggca
     4141 cccgaacatg cacttcactc taacccactg ggatatcacc agctccgagg gccgcaccca
     4201 ctggtggtcg tgaaacccgg cctcggccag tatccgggtg acaccggcgg cggcatcttc
     4261 attcatcttc ctcgccctcc tcgtctcccg ggggccggga caaaaagggc cccctcaagg
     4321 tcaaccatta tagcaaggaa gaaaggacgg atgacaaaac ccttcgggcc aagtagaatt
     4381 ctctgaacgg ggatatgacg gacgcgaggg ggttagccga gttgtcaagg tcaaaacggg
     4441 gcttcacctt ggtggagatc cttctcacga tacttatcat agccatcctc gcagggaacc
     4501 tgcttttgac ctcgggcata tcgagggacc ggtccgccgc gacgaggatc gtctcggacc
     4561 tgatgaccct caagaggacg gccatgctgt tcttctccga taacgatcgc tggcccggag
     4621 agagcgacta cgaaaggctc aacgcctact tgagcggcca catccagctc caggcctggg
     4681 acgaggattc gggggtctac gccatcaaga ggcacgaagc cctggggacc gtttacgccg
     4741 ccgcgagggt ggccgatgaa ttcggcggtt cgccccgcat aagggagttc ctggaaaggg
     4801 actcctccga gtacggcctc ctcaacgggg agctgtcccc ctatcaaagc gtcgaccccg
     4861 tcgtggctgt gagggtaaag gacatcgggc cgggatccga cgaaggcgga gaggatgatc
     4921 actccgggcc ggaaggcccc ggcggaggcg ccgggcagga ctccgggtcg ggcaccggag
     4981 gcaaccaccc cgatcatgat acatgggaca gcggccgggc ttacctggga ggcgaacggg
     5041 tcgtattcga cggcggggtc ttcgaagccc gctattggac ccaggggaat caaccgggcc
     5101 tgacggacag cccctggcag gagatcaccg atgaatggag agacttcaac gaatacaagc
     5161 agggagacat agtagtttac aacgggaaac agtaccaggc ctggtactgg acgaaggata
     5221 acacccccgg cgtggtgggt tccccgtggc aacaggtcgg cactgaccaa tgggtcgcgt
     5281 cgaacaaata tgccggcggc gattttgttt attatgacgg gaaacagtac aaggctaaat
     5341 ggaacgtaga ccccgggaag aacccgccgg ggtcatccga cgcttgggag ttgatcccat
     5401 gagtatcacc cccggggagg gatcgcaatg gctacggtga ccgccgtctg cgccggcgag
     5461 gaccggcaaa aacccaagaa cgccctcgag gcggcccgct tcgtcgaggg gatgggcatc
     5521 gccggcgacg gacatttcgg gatgggggac cgccaggtga gcctcctccg ctccgaggat
     5581 ttggccctgg cggagtccga ggccggtttc cccttccccc ccggttccct ggcggagaac
     5641 ctcgtggtcc gtgggctgcc gacggaactg gccccgggat ccctgctcag gataggggcc
     5701 gaggtcgtcc tggaagtggt cgaaaagggg aaaaggcccg atgagcccca ctcctacgac
     5761 taccgggggt ggtgcctcct gccgacggcg ggctacttcc tgaaggtgct gaccgggggc
     5821 gaagtgcgcc ccggcgacac cgtgatcctg gagggaaaag caccctaggg gagccgaccc
     5881 ctcgaggccg cgggcctcgg gcaggtcagg gcgcgccggg tgaagcgata cgggtcagcc
     5941 cttcgatgaa aagcgccgtc ccctcgacgc cgtccagccg gtaggtcgcc agggtacccc
     6001 tatcacttcc cgccccggcg acgatgaccc ccgcccctct tcctgcgacg gccctgaagc
     6061 cgtcctcgtc ggtgatgtca tcccccacgt agagagcaaa ggaaccggcc tgggtcatcc
     6121 cgagacggtc gaggagccat aggacggcgg aacccttgtt ccagtccatg tcgggcagca
     6181 cttccacggc cttcttgtcg ggcctcgccc tcaagccccc gacgtcggcg gccgcctcct
     6241 cgatgaggcc gatcaacgac cccacatcct cctcggccac gagcctgtag tggaccgtca
     6301 ggcagtaccg tttcctctgg aggatcgcgc cggggaaacc tcccacgatc ttttcaaagc
     6361 gttcgcccgc ctcttccagg acatgggtgt actcttcccc cgtccggaag gcgacaccgc
     6421 tccccgaggg accggctatc tcgaaaccgt ggcatccgga gtaaaaaagg ccaggaatcc
     6481 ccaccctcct tcggatatct cccaggtccc tccccgtcac cacggcgacg gcggcgaccc
     6541 tggaaagggt ttccagcctc gacctcatgg ccgggtcaag gtaagcctcg taggggtcct
     6601 tcacgatagg tgttagggtg ccgtcgaagt cgaaggcggc gaaaatccgc cttccccggg
     6661 cggcttcctc gatgagcccc atgctatcca gcgccgacgg gagcctgcac ggatcagcgc
     6721 cggaggatcc tccctccttg gcggacggcc tgccggtctc acccattctg cttccccgtc
     6781 atctcctcga catcgaccct gatgatcact atcctcgcca aggcctggtc tgggaactcg
     6841 tactccttcc ccatgtagtg tcccatgatg acattcatcc cgcgggcctt ttcctcgggt
     6901 tccctcagga cttcggccct cccccaggct atcacgctct tgtagtacat ggtgaaatcg
     6961 caggcttctt gagcgtactt catctgcagg tccgcctcga attcgagaca gacatagggg
     7021 tttttcccga tgacatcgag ttttctcccc tccggtgcgc agtgcatgta taccttgcca
     7081 tcacggtacc cgaagttcat gggcaccact tagggcctgt ctccatcaca caaacccatc
     7141 ctgcatatgg aagaccccct tatgatcttc tccatcgtcg ccaggtcctt tatctccctt
     7201 tcattccttc gcatctcctt aacctcccat ttttcaaggt aaagattttt cacagcccga
     7261 cgccttacaa tttaacatga tcaggcattc cctaaaaagg ctcaattgaa gaacccgaca
     7321 gggagtaata ggtcccgctg tcgtccgggt gctcgaggcc tgcacgagca aggggtcttt
     7381 accccggtcc cgagagaagg tgctgacagg gatggctttc gtgccgacgc ttgtatcggt
     7441 agcggatagg atcgccatgt tggctgcaca aaagggatgc ggcccaatgc gaaaccctaa
     7501 agggccccgt gataaaggca gccgggcagg cccttggaat accgggatcg aaatgtccat
     7561 gatcatcaac tccatgcaca gttagaacct gagacatccg gaccagccga taacccacag
     7621 gggctgtctg tggattatcg gtatctcggc ggtatccttc ctgttccact cccaaggtat
     7681 gctcttgagg tccggcatag aatacccttg tgcctttcat cgtattctcc cggggacaat
     7741 aactactccc actcttgtct tgaaaaacta caatcgcaag ctcctgtgta ctattgattt
     7801 tttttgcgat aaatatttat actgttaaca ggtggaacaa aaagtacttt cacgaaccta
     7861 ccaatgggag gtgagctcca gtgagatgtc cagtaagagt agtattcatt gccgttcttg
     7921 tctccctttt cgcttttccg gcgtttccat ccgatgtgcc cggaagcaag gatcaccctc
     7981 ttctgccccg gatcacgggt tactacatag gagactacca ggaggtcgaa ttcggttcac
     8041 acgactttat cgacgccgaa ggcgactatc tgacggtgga aggacgccgt atccagatcg
     8101 aatacgacat caacgaaggg gagtcacctc cgggcgatct tttcgtgata gtgaactacg
     8161 aaagggccgc ctcgggagca ggaggcagtt tttaccgtca cagggatgat atgacctacc
     8221 tgacacttca acaggacagc acagagacct gggcggaggt catgagccag ggtgacggcc
     8281 agacctattg gctggtcatc gtcgaaaagg gagtggctga acagcggatc tccgccaatg
     8341 aaatggcccg ggccctgaaa accgcaggaa gggtatccct ttcgatccat tttgataccg
     8401 gcaaggctgt catcaggact gaatcacagg cgatcatcga cgagatcttc cagatgatga
     8461 ccgaggatgg ccggatgagg ctcaaggtgg aaggtcatac cgattcggtt gggaatgaag
     8521 ccgataacct ggcgctttcg aaggaccgcg ccatggcggt ggtccagtca cttgtagaaa
     8581 agggcattga acccgagcgc cttgaatacg agggttttgg agaattgcgc ccggtatccg
     8641 acaatgaaac ggcatccgga agggctaaaa accgcagggt cgacctggtc ctgctttaag
     8701 gcccaacgcc atgtccaaca gggcagttga acgcatcgcc cttgcggttt tgctcgcttg
     8761 cgcagtaatt gtcggcattc attcgggtgc gcaaggagca cttcccgaca gtattcccgt
     8821 gtcgatccac ctggaagcgt cctggaacac cgatgaaggg gattcccaac tcttcgagac
     8881 ggggtcgtgc agcataaatg tctcgggaca gatggtcagg agcgcaggcg acaacatctt
     8941 tttctcccat tacatccctt cgggcgtcac ggcttcctac cgttacgatg aatctatcat
     9001 agcccgccca tccgacgaag agagcgaatg ccgaggggag ataggcaagg tctttgcttc
     9061 cggttccatc gatgtcggtt ttgagccggg aaccgacaag gcatctctct tcttgcagtc
     9121 cttcaccggc ccgcttgggg tcggcgcatt tcttcaggct acggcagacc cgattatcct
     9181 cgaccccgaa agccttatcg agagaatggc gaatgatccc gacaggactg tctactcctt
     9241 cttccttatg gtcccggtta cagcgtcggt ggaagaggtg agcgggtgcg aaaccctgtt
     9301 cacaaagacc cttgatctgg cccttgctgc aaacgctgtt tcggaaatgg gcattaccgg
     9361 gttgaccggc agttattcct gggatgcgga ggatccgcca aaaagcgggg catctctttt
     9421 cgcggtcggg gaaaagatcc attacggccc ggaaccaggt acgaaaaaac acggcagcgt
     9481 gactttctca tggaccttcg gggaaattga accagtggta gccctttact gggtaaagga
     9541 gaccgaaagg gtcgaattca ccggtgaaga gatcgaggta atggcaggaa ggaaggtaag
     9601 gctcgaggcg gaagtttacc cccaggccga tgaagcctca gcagggcatt gggagctccc
     9661 gccctccttt atcgcgggcc tcgaggggta tgaccgcggg aacttcgcca ggggtaaggc
     9721 tatcctactg tccgaagaag aaaaacgaag taggcggatc gatttctact gcctggacgg
     9781 ggctttccag ggcaaagacc aaaagatagc ttatgctgcg acgataaagg gggaaaaggt
     9841 cgaagggaaa gggacggtga aagtattttc ccccgaagcg gacatcgacg tcatcccgga
     9901 aaagagggtt tcgataggtg gcgtttcctc gggtaaagga tgctcggcat acctcggaag
     9961 gatagccccg cccttgtcca tcccggggat agagatcaag ggcaaggcaa gattcccggc
    10021 cggtttcgaa aaggaagagg cggaacactc catctgtttc gtccagttgc tcagggagga
    10081 catcctcgaa aggaaaggca ggttcgggca acagggaggt gactacttcc agcttgtatc
    10141 ggagaatttc tctcttgaca caaggttccc ctacggcggg ctcgaaggga aagaaagcct
    10201 tgaaatgaac gacgttccca gcgacgagat cggcccactg acccagaata tccatcattt
    10261 ccaggatttc cgcactaccc ttcttataag gcccgggccc ggcgggaagg ccatctggct
    10321 gccgatcgag accatcccct ggtcgtggcg gttttccctt gagagggtga aggacaatcc
    10381 agggtgggac gagacctgtc tgcccgggaa cttcaggata accaataccc gtttcgtcat
    10441 tccaggaaaa agagaaaagt tcagcggaaa gctggaggac gtcccttcat gggacaaggt
    10501 cctggatggc tccgaaaagg ccatgcatca taatgacgac aaccggtggc tggacgatgt
    10561 caataccttc aggaaaataa aatagaacag gcgctgaaag ctgtcgggat caaaactatc
    10621 cattattagc aaccccatgt acaggtcgaa cctgtctttc ggggagttaa gggtagtgct
    10681 tattagtttg tgtaaacctt cattgttggg gagaattgat atc
//
LOCUS       DAOSRG010000027        10507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6178_length_10507_cov_7.512055, whole genome shotgun sequence.
ACCESSION   DAOSRG010000027 DAOSRG010000000
VERSION     DAOSRG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_10507_cov_7.512055"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLM30_02020"
     CDS             <1..666
                     /locus_tag="PLM30_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR53978.1"
                     /translation="LLVTDGVFSMDGDIAKLPEIVELCEAYGVIVVVDDAHGEGVLGR
                     GGRGIADHFCLHGRVDVEVGTMSKAFGVMGGMIAGSATLIDYIRQKARPNLFSSALTI
                     PDVAANIASVDILEESEERVNRLRENGDFLKRELSAAGFDTGHSETPITPVIIGEASD
                     AKTFSAELFKKGVFATAIVFPTVPRGTARIRCMVSAAHTKEDLRFAVERFVEVGRGMG
                     ILP"
     gene            663..1625
                     /locus_tag="PLM30_02025"
     CDS             663..1625
                     /locus_tag="PLM30_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR53979.1"
                     /translation="MKRILVTGSNGQIGAELVPYLRERYGAENVLATARRRVPGPVSE
                     GGPFEILEVRDGRAFSGLMGSFGVDAVIHLAGVLSARAEADPQGSWDVNAGGCFAALE
                     CARERGAAFFFPSSIAAFGPSTPADGTPQVTIQRPTTIYGVAKVTGELLCDYYHLKYG
                     LDTRGLRFPGLISYEAPPGGGTTDYAVHIYYEAVSQGRYTSYIAPGTFMDMMYMPDAL
                     EAVVRLMEAEPSKLVHRNAYNVSAMSFEPGQVAASIRKRIPSFEIDYAVDPVRQAIAA
                     SWPRSLDCSAARAEWGFAPKYDLDAMTADMLAKIQRRGQVYTFD"
     gene            1831..2928
                     /locus_tag="PLM30_02030"
     CDS             1831..2928
                     /locus_tag="PLM30_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR53980.1"
                     /translation="MGNVILDLDESRIVIPKDRRLRKVYLEFSTRCNLNCPMCFRESW
                     TEPQGDMDWGLFLRVASDLAQFPLLETVHFLGMGEPFCHPRLFEAVGILKSGGRRVRI
                     TTNATLLDGAAARALVAVGADIIDLSVEADDVPWPGRVSSLEILSKAVEELREARKHA
                     GSNACEIRIALVVTRSNFPLIPGIVRSLKGKGISAVSVSNLLPTDMEADANRLYPLTL
                     EEEVAVRSKLYTQFQYAGIKPELPRFSLSTERHCNFVRSESCFIRWDGLVAPCLRLCH
                     GYREIVLGREKEVIPWFFGDLKRQGLAEVWDDPGYVSFRWRLRRALYASCLDCNLLRS
                     CEYAKTTESDCWGNSPACSDCLWDRNLIRCP"
     gene            3075..4871
                     /locus_tag="PLM30_02035"
     CDS             3075..4871
                     /locus_tag="PLM30_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOR53981.1"
                     /translation="MQAKRLLRVDLSAGTVKQEPLNPDWVKQYIGGRGYGTKLLYEEL
                     KPGTDPLSEGNKLIFATGPLTGSNVPTGGRYMVITKSPLTGFIASSNSGGYWGAEMAR
                     AGYMMILVEGKAKKPVYLWINDDKAELRDASSLWGKDTHETTDALLKEVGDPKARVAC
                     IGPAGEKLSPIACIVNEKHRAAGRSGVGAVMGSKNLKAVVVRGSGAFKAADPEGLRET
                     LKAAMQKIKENGVTGEGLPTYGTAVLVNILNQLGSYPYKNFQASQHEGAEKHSGERLA
                     ETFLTGRTNCFACPIGCGRETALEDKKGEGPEYETLWALGSNCGVDDLEPIIRANYLC
                     NELGLDTISTGGTISAAMELYEKGFIPKEDLGVGPELKFGSGDALVYWTEKMGKVDGL
                     GERMAQGSYRLGEYYGHPEFSISVKKQELPAYDPRGIQGIGLTYAVSNRGACHVRGYL
                     ISPEVLGIPEKLDPWVTEGKAQWGKIFHDLTAAIDSSGMCLFTSFALGAGDYADMLKH
                     GAGFDLDADSLLEAGDRIWTLERLFNLREGLDPARDDTLPARLLKEPIPDGPAKGKVS
                     RIGEMIPEYYKLRGWDEKGVPTPSKLAALGIR"
     gene            4881..5153
                     /locus_tag="PLM30_02040"
     CDS             4881..5153
                     /locus_tag="PLM30_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HOR53982.1"
                     /translation="MIRVSFFGMVRGIVGEKETQVETEGTVADLLEVLSGKYGLRFGE
                     KVLKTPERELQLILILNGRHIEHIGGLAAPLKEGDHLAVFPLVGGG"
     gene            5465..6394
                     /locus_tag="PLM30_02045"
     CDS             5465..6394
                     /locus_tag="PLM30_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOR53983.1"
                     /translation="MQKRVWGRRWGVRNDVGTLKTVLLHRPGPEIGVMTEDHYDPELE
                     ALINDEEQWYFRSDKAPDLARMQEEFDGLTAILQREGVEIVWAEGGPRDPNAVFVRDN
                     AMIVDGGAVLARMGPVGKEYGTGRRGEEFFIYRKLAEIGMPVLHIIHGDGLMEGGSFC
                     LLNEKTAVVGLSFRGNRSGADQLRSVLATQGKGLIEIPLPGYAMHLDGGIVMVDHDKA
                     AVNVERLPYWFLETLQELGIKAIPLDHRESGLAINCLTIAPGRVIMDSGCPWTSERLE
                     KNGVEVLSTPWSEISKHGGGIHCSTLPLVREDD"
     gene            6558..7364
                     /locus_tag="PLM30_02050"
     CDS             6558..7364
                     /locus_tag="PLM30_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR53984.1"
                     /translation="MNKKWFCVLAGILAIAMMSGVAFADTPSTLDTVQKAKKIVIGSA
                     PGYLPFEMMDKQGNFIGYDIDLGRAIAKSLGVEVEFRQFEFSGLIPALQTGDIDMFIA
                     GMTIRGDRALAVSFSNPYYATGQVLMVPGSDTATKSWQDLDKPGKRIALSQGTTGALL
                     AKSIFKEAKILDFPTFPEAAAAMISGNADGIIYDEPGIRMYQVMHEGAVRGIYDLISQ
                     ENLGVAVRHNDFAMIQWLNSFLYQYIGSTEELALRTKWFETSDWMEEMKQ"
     gene            7448..8101
                     /locus_tag="PLM30_02055"
     CDS             7448..8101
                     /locus_tag="PLM30_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOR53985.1"
                     /translation="MYIADWGVIARNLPMFLNGAYLTLKISLIALVLSIPVGAVVGLC
                     RISRNRIVSFAAACYVEFFRGVPLLVLLMWIFFVLGRFLRLGSYWSAIIGLAIFSGAF
                     VAEIVRAGIEAIPRGQMEAARASGMTYGQAMKLVILPQAVRKVLPPLASQFIILIKDS
                     SLVSVISVVDLTLIGKNLVATTFRSIEIFSFIAVLYFCMTFSLSKFIGFFERRTIRE"
     gene            8112..8855
                     /locus_tag="PLM30_02060"
     CDS             8112..8855
                     /locus_tag="PLM30_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOR53986.1"
                     /translation="MMTDVVFSIRNVSKSFGTLQVLKNVTFDIPKSKVFAIIGPSGAG
                     KSTLVRTLNGLEGINGGKIFFEGICVQDKKTNINKLRMSIGFVSQSFNLYPHLSVLNN
                     VTLAPIHVRGLPRREADEKGRSILASLGLADKELSYPSQLSGGQQQRVAIARALAMEP
                     HVILFDEPTSALDPELIKEVLDAIRQLAKTGMTMVVVTHEMSFAREICDQIVFMDHGV
                     VLEITPPLEFFSEPKTPRAKEFLSKILHH"
     gene            complement(9258..9410)
                     /locus_tag="PLM30_02065"
     CDS             complement(9258..9410)
                     /locus_tag="PLM30_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53987.1"
                     /translation="MVGVRVFVSMRHSIVEIDKISFFEIYRFLVSVKEPDPPFKDIKK
                     LLTIVT"
     gene            9503..10453
                     /locus_tag="PLM30_02070"
     CDS             9503..10453
                     /locus_tag="PLM30_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOR53988.1"
                     /translation="MECSQVLIHGERWFPGGKRFSEDMPEYWGDWYCDSEVGKLRAVL
                     MHRPGPEIDAVTDPASVRFKALMDPEKAREQHDALAGIYRDHGVRVYYVEEGRLDKPN
                     ALFMRDQVLMTPEGAIVCRPAMEVRRGEERFAAETLARLGVPILKTISGEGVFEGACL
                     LWVDRKTVILGTGCRANKAGALQVERELRDLGVDTILYFQIPYGHAHLDGLMNFADRK
                     TAMIFPSQVPYDVCKPLLDQGFRIIENPYVDEVELTSGINFVALEPGKVVIPSGNPRT
                     CALLGEAGVECVEVDITELLKGWGAIHCMTAFLKRDPIEA"
BASE COUNT         2232 a         2983 c         3210 g         2082 t
ORIGIN      
        1 ctcctcgtca ccgacggggt cttctccatg gacggcgaca tagccaaact gccggagata
       61 gtcgaactgt gcgaagccta cggcgtcatc gtcgtcgtcg acgacgccca cggggagggt
      121 gtgctcggcc gggggggccg aggcatcgcc gaccacttct gccttcacgg cagggtggac
      181 gtggaggtgg gcaccatgtc caaggccttc ggggtcatgg gcggcatgat cgccgggagc
      241 gccaccctca tcgactacat ccgccagaag gcccgtccga acctcttcag cagcgccctc
      301 accatccccg acgtggcggc caacatcgcc tcggtggata tactggagga gagcgaggaa
      361 cgggtcaacc ggctcaggga gaacggcgac ttcctgaaga gggagctctc ggccgcgggc
      421 ttcgacacgg gtcacagcga gacacccata acgcccgtca tcatcggcga ggcctccgac
      481 gccaaaacct tcagcgccga actcttcaaa aagggcgtct tcgcgacggc catagtcttc
      541 cccaccgtcc cgagggggac cgccaggatc cgctgcatgg tctcggcggc ccacacgaag
      601 gaagacctcc gcttcgccgt ggagcgcttc gtcgaggtgg gccgagggat gggtatcctg
      661 ccgtgaaacg gatcctcgtc accggttcca acgggcagat tggcgcggaa ctggtaccat
      721 acctgcggga gcggtacggt gccgaaaacg tcctggccac ggccaggcgc agggtgccgg
      781 ggccggtctc ggaggggggg cccttcgaga tcctggaggt ccgcgacggg agggccttct
      841 cggggctgat gggctccttc ggggtcgacg ccgtgataca cctcgccggg gtcctgtcgg
      901 ccagggccga ggccgacccg caggggtcgt gggacgtcaa cgccggaggg tgcttcgccg
      961 ccctggaatg cgcccgggag cggggggctg ccttcttttt tcccagctcc atcgcggcct
     1021 tcgggccctc cacgccggcc gacggcacgc cccaggtgac gatccagcgg cccaccacga
     1081 tctacggcgt cgctaaggtc acgggggaac tgctctgcga ctactaccac ctgaagtacg
     1141 gcctcgacac aagggggctg cgcttccccg gcctcatctc ctacgaagcc ccgcccggcg
     1201 gcggcaccac ggactacgcc gtccacatct actacgaggc cgtctcccag ggccgttaca
     1261 ccagctacat agccccgggg accttcatgg acatgatgta catgcccgac gccctcgagg
     1321 cggtggtccg gctgatggag gccgagccgt cgaaactggt ccaccgcaac gcctacaacg
     1381 tgtcggccat gagcttcgag ccggggcagg tggcggcctc gataaggaaa cggatccctt
     1441 ccttcgagat agactacgcc gtcgaccccg tgaggcaggc catcgccgcg tcgtggcccc
     1501 gctccctgga ctgctcggcc gccagggccg aatggggctt cgctccgaaa tacgacctgg
     1561 atgccatgac agccgacatg ctcgcaaaga tccaaaggcg gggtcaggtc tacacttttg
     1621 actaaatgcc ggatcccacg gaaaaggcag accccaagag caaaggacgc tctttgttta
     1681 agtggatttt ttccaccgcc gtgtaaaaag tcaaaagtgt agacctgacc ccgctccttg
     1741 acgtagggac gctttaatct gataataaaa tagtcattgt cagggatgcg ggctgtcggg
     1801 atcgacggcc cccaaggggg gtccatgaca atgggtaacg ttattcttga tcttgacgag
     1861 agccggatcg tcatccccaa ggaccgacgg ctccgcaagg tctatctcga gttcagtacc
     1921 aggtgcaatc tcaactgccc catgtgcttc cgggaatcct ggacggagcc ccagggtgat
     1981 atggactggg gcctgttcct ccgggttgcc tcggacctcg cccaattccc ccttcttgaa
     2041 acggtccact ttcttggtat gggagaaccc ttctgccacc ccaggctctt cgaggccgtc
     2101 ggcatcctca agtccggcgg ccgccgtgtg aggataacga ccaacgccac cctgctcgat
     2161 ggggccgccg ccagggccct tgtggccgtg ggggccgaca tcatcgacct ctccgttgag
     2221 gccgatgatg tcccgtggcc ggggagggtg agcagcctgg agatcctgtc gaaggcggtg
     2281 gaggagctcc gggaggccag gaagcatgcc gggtccaacg cctgcgagat ccgcatcgcc
     2341 ctggtggtca cccggtcaaa cttccccctc atccccggta tcgtgcgatc cctgaaaggc
     2401 aagggcatca gcgccgtctc ggtgagcaac ctgctcccca ccgatatgga ggccgacgcc
     2461 aaccggctct atcccctcac cctcgaagag gaagtggccg tgcgttcgaa actctacacc
     2521 cagttccagt acgccgggat caagcccgaa cttccgaggt tctccctgtc caccgagcgg
     2581 cactgcaact ttgtgaggtc cgagtcctgc ttcataaggt gggacggcct cgtggccccc
     2641 tgcctcaggt tgtgccacgg ctatagggag atcgtcctcg ggagggagaa ggaagtgatc
     2701 ccctggttct tcggcgacct gaaaaggcag ggactggcgg aggtctggga cgacccgggt
     2761 tacgtttcct tcaggtggag gctgaggagg gccctctacg cctcctgcct tgactgcaac
     2821 ctcctcaggt cctgcgaata cgccaagacc accgagagcg actgctgggg taactccccg
     2881 gcttgcagcg actgcctgtg ggaccggaac cttatccggt gcccctagaa gatcacccgg
     2941 taggggaaaa ggaggtgacc gacgggtcct tcgaagtatc caggctgact tcctgagcga
     3001 agaatagaga gtttcgagag ttctttaccg tgggacggca aggttgtcag aaaaagagaa
     3061 aaggagggaa agacatgcaa gcgaaacgtc ttttgagggt tgatctttcc gcgggaacgg
     3121 tcaaacagga accgctgaac cccgattggg tcaagcagta catcggcggt cgcgggtacg
     3181 ggaccaagct tctctacgag gagctgaaac ccgggacgga ccccctctcg gaggggaaca
     3241 agctcatctt cgccacgggt ccgctgacgg gttccaacgt ccccaccggc gggaggtaca
     3301 tggtgatcac caagagcccc ctgacaggtt tcatagcctc gagcaactcc gggggctact
     3361 ggggggcgga aatggcaagg gcgggctaca tgatgatcct cgttgagggg aaggccaaga
     3421 agcccgtcta cctgtggatc aacgacgaca aggccgaact gcgcgacgcc tcttcgctct
     3481 ggggaaagga tacccacgag accaccgacg cgctgctgaa ggaagtgggt gaccccaagg
     3541 cgagggtggc ctgcatcggc cccgccgggg agaaactatc acccatagcc tgcatcgtca
     3601 acgaaaagca ccgggcggcg ggccgcagcg gcgtgggcgc tgtgatggga tcgaagaacc
     3661 tcaaggccgt cgtcgtcagg gggagcgggg ccttcaaggc cgccgacccc gaaggtctca
     3721 gggaaaccct gaaggcggcc atgcagaaga tcaaggagaa cggcgtcacc ggcgagggtt
     3781 tgcccaccta cggcaccgcg gtcctggtca acatactcaa ccagctcggc agctacccct
     3841 acaagaactt ccaggcgtcg cagcacgaag gcgccgagaa acactccggc gagaggctgg
     3901 cggagacctt cctcacgggg aggaccaact gcttcgcctg ccccatcggg tgcggaaggg
     3961 agacagccct ggaggacaag aagggcgaag gccccgagta cgagaccctc tgggcgctgg
     4021 gcagcaactg cggcgtcgac gacctcgagc ccatcatccg tgccaactac ctgtgcaacg
     4081 aactggggct ggacacgatc agcaccgggg gcacgatctc ggcggcgatg gaactctacg
     4141 agaaggggtt tatccccaag gaggaccttg gcgtggggcc ggaactgaag ttcggctccg
     4201 gcgatgccct ggtctactgg acggagaaga tgggcaaggt cgatggtctc ggggagagaa
     4261 tggcccaggg cagctaccgg ctgggggaat actacgggca ccccgagttc tccatctccg
     4321 tcaagaagca ggagctcccg gcctacgacc cgcggggcat ccagggcatc ggcctgacct
     4381 acgcggtgag caaccgcggg gcctgccacg ttagggggta cctcatttcc cccgaggtcc
     4441 tgggcatccc cgaaaaactc gacccctggg tgacggaggg caaggcccag tggggcaaga
     4501 tcttccacga cctgacggcc gccatcgact cttcgggcat gtgtctcttc acatccttcg
     4561 cgctcggcgc tggtgactac gccgacatgc tcaagcacgg ggccggcttc gacctcgacg
     4621 ccgacagcct cctcgaggcc ggggacagga tctggaccct ggagcggctc ttcaacctca
     4681 gggaggggct cgaccccgcg agggacgata cattgcccgc caggctgctc aaggaaccca
     4741 tccccgatgg gcctgccaag ggcaaggtga gccgcatcgg cgaaatgata cccgagtact
     4801 acaagcttcg gggctgggac gagaagggcg ttcccacgcc ctcaaaactg gcggcactgg
     4861 ggatccggta gggggagggt atgatccggg tctccttctt cggcatggta cgagggatcg
     4921 tcggggagaa ggaaacgcag gtcgaaacgg aaggtaccgt ggcggacctc ctggaggtcc
     4981 tgtcgggaaa gtatggcttg cgcttcggcg aaaaggtcct caagacgccg gagcgcgagc
     5041 tgcaactaat cctgatcctc aacgggaggc atatcgaaca tatcggcggg ctggcggccc
     5101 ctttgaagga aggagaccac ctggccgtct tccccctcgt cggcggcggt tagttaccgc
     5161 cggggcgtct ctcacagagg ggctcttccg cgatgatgcg ggagggcccc ttcttttcct
     5221 gaagatcttc atttttttac aatgatgtat atggctaaac aggtgattga caagagtcct
     5281 cgaaatgtta ttctgaaata gtttactggg aagaatatat tttcgttaga gtaaaagaaa
     5341 cttcaccatc aaaaacacca tccaggggag gtcaggcatg agcctacgaa acagctccca
     5401 gtattatcac gttgttctgc agcgtatacc ccctcgcccg ctcccgcctt ttgaagaccc
     5461 ggacatgcag aaacgggtat gggggcggcg ttggggcgta cgcaacgatg tggggaccct
     5521 taagacggtc ctccttcata gacccggccc ggagatcggt gttatgacgg aggaccatta
     5581 cgatccggag ctcgaggcac tcatcaacga cgaagaacag tggtacttca ggtccgacaa
     5641 ggcacccgac ctggcgagga tgcaggaaga attcgacggt ctgacggcaa tcctgcaaag
     5701 ggagggcgtc gagatcgtct gggctgaggg cggcccccgc gaccccaatg ctgttttcgt
     5761 aagggataac gccatgatcg tcgatggcgg ggctgtcttg gcccgtatgg gaccggttgg
     5821 gaaggaatac ggaacaggca ggcgtggtga ggagtttttt atataccgca aacttgcgga
     5881 gatcggcatg ccggtgcttc atatcatcca cggtgacggg ctgatggaag gcggaagctt
     5941 ctgcctcctc aacgaaaaaa ccgctgttgt gggcctaagc ttccgcggca accgctctgg
     6001 ggcggaccag ttgaggtcgg tgcttgctac ccagggcaag ggcctgatcg agattccgct
     6061 tcctggatac gccatgcatc ttgatggagg cattgtgatg gtggatcacg acaaggccgc
     6121 cgtcaacgtt gaacggttgc catactggtt ccttgagaca ctccaggagc tcgggatcaa
     6181 ggccataccc ctggatcacc gtgaaagcgg cctggcgata aactgcctga ctatcgctcc
     6241 aggcagggtc atcatggact ccggctgccc ctggacttcc gaaaggcttg aaaaaaacgg
     6301 tgtggaagta ctatctacac cctggagcga gatctcaaaa cacggcggcg ggatccattg
     6361 ctcaacgcta cctctggtca gggaggatga ttgagtcgag ggatcgggac tcgtcggttc
     6421 ttttcccggc gaccctatag tcaatggaag gaggtggacg gattcggtag agagaaaaaa
     6481 agcggtaagg cccagcgctt aggggtttgg agagtttatc cgaagaagtc cacgatggaa
     6541 agtaggaggt gtgagcattg aacaagaaat ggttttgcgt gctggcaggg atccttgcga
     6601 tcgccatgat gagcggtgtc gctttcgccg atacgccttc gacgctcgat acggtacaga
     6661 aagcgaaaaa aattgtcatc ggctcggcgc cgggttatct tcctttcgaa atgatggata
     6721 agcagggtaa tttcattggc tatgacatcg atcttgggcg ggccattgcg aaatccctag
     6781 gtgttgaagt ggagttccgc cagttcgagt tctccggttt gatcccggcc ctacaaacgg
     6841 gtgatatcga catgttcata gccggtatga cgatccgtgg cgacagggcc ttggcggtga
     6901 gcttttccaa cccgtactac gctaccggac aggtgctcat ggtccccggt agcgacacgg
     6961 caaccaagtc ctggcaagat ctggacaagc caggtaagag gatcgccctt tcccagggta
     7021 ccaccggggc cttgctagca aaaagcattt ttaaagaagc caagatcctt gacttcccca
     7081 ccttccctga agccgcggcc gcgatgatca gcggtaacgc cgatgggatt atctatgatg
     7141 aaccgggcat acgcatgtac caggttatgc acgaaggcgc cgtcaggggc atctacgacc
     7201 tgatcagcca ggaaaacctg ggtgttgccg tcaggcacaa cgatttcgct atgatccagt
     7261 ggctcaattc cttcctttac cagtacattg ggagcactga ggagctggct ctacgtacaa
     7321 agtggttcga gacttccgac tggatggaag aaatgaagca gtaacgaccc ggcaagggca
     7381 aacgcccccg ggtgagcctt tgcccttgcc cgggggcctc actttgaggt gttaaggagg
     7441 gggttcattg tatatagccg actggggcgt gatcgcccgc aacctcccga tgttcttgaa
     7501 tggggcctac ctgaccctga aaatatctct catagccctg gtcctcagta ttcctgtggg
     7561 ggcggtagtg ggtctttgcc gcatatcgag gaaccggatc gtatctttcg cggcggcctg
     7621 ttacgttgaa tttttccggg gggtgccgct gcttgtcctt cttatgtgga tcttttttgt
     7681 cttggggcgc ttcctgcggc tgggttccta ctggtccgcc atcatcggcc tggcgatatt
     7741 ttccggggcc ttcgtggcgg agatcgtccg agccggcatc gaagccatac ccaggggaca
     7801 gatggaggcc gcccgtgctt ccgggatgac ttacggccaa gccatgaaac tggtcatcct
     7861 ccctcaggcc gttaggaagg tattgccacc cttggcttcc cagttcatta ttctcatcaa
     7921 ggattcatcg ctggtctcgg tgatctccgt tgtggacctt accctgatag ggaaaaacct
     7981 cgtggccacc acgttccgct ccatcgagat cttttctttc attgcagtcc tttacttttg
     8041 catgaccttc agcctgtcga agttcatcgg cttcttcgaa agaagaacga tcagggaatg
     8101 atggggtatt gatgatgacc gatgttgtct tttccataag gaacgtctcc aaaagcttcg
     8161 gtactctcca ggtactcaag aatgtcactt tcgacatacc gaagtcgaaa gtttttgcca
     8221 ttatcggtcc tagcggggcg gggaagagca cccttgtgcg taccttgaac ggactggaag
     8281 ggatcaatgg aggcaagatt ttttttgagg gtatctgtgt tcaggacaaa aaaacaaaca
     8341 ttaacaagct gcgtatgagc ataggcttcg tgtcccagtc tttcaacctc tatccgcatc
     8401 tgagcgtact aaacaatgta acgctggcac ctattcatgt caggggcctg cctagaagag
     8461 aagcggacga aaagggcagg tccatcctcg cctcgctggg tctcgccgac aaggaactgt
     8521 cctatccaag ccagctttcc ggggggcaac aacagagggt tgccatcgcg agggccttgg
     8581 ccatggagcc tcacgtaatt ctcttcgatg agccgacttc cgccctggac ccggaattga
     8641 taaaggaagt cctggatgcc attcgtcaac ttgcgaaaac cggcatgacc atggtagtcg
     8701 ttactcacga aatgagtttc gcgagggaga tctgcgacca gatcgttttt atggatcacg
     8761 gggtggtcct tgaaataacc ccgccccttg agttcttctc agaacccaag acaccgaggg
     8821 caaaagagtt cctgagcaag atcctgcacc attgacgtgt ggaagtgaat ccaaagcgag
     8881 aaggggtgac ggctctgacc caagtaagta aaaacctccg caagtttcgt ttgagccgag
     8941 gactgtccct ttcaaaactg tcaggaattg cttcagtttc ggtagcacaa ctctctaaac
     9001 tcgagaatgg gaaagtctcc ccgagcctgg cggtccttcg caggctagcc gatgctttgg
     9061 aagtatcctt ggccgctttg gtggccgaaa aggtcccccc ccagcttaac cccgtttttc
     9121 gcggtgaagg atacaccttc aggcgggaca cgccggggca gaaacaaata gaggaagtat
     9181 tccttcacat atcccaggaa gcccgcatgc agcccgaaat catgactttc cctcctgggt
     9241 ccgaaagcgg ccatgcccta agtcacgatg gtgaggagtt tttttatgtc cttgaagggc
     9301 gggtccggtt cttttacgga aaccaggaac cggtagatct caaagaagga gattttatct
     9361 atttcgacaa tactgtgcct catcgatacg aaaaccctga cgcccaccat cctatgaggc
     9421 tcctggtctg ttgctcgccg ccggttttct aggaacaaca agtgcattga gatctgaatt
     9481 ctcttttggg aggtcgcaag ggatggagtg ttcgcaagtg ctgatccacg gggaaaggtg
     9541 gttccccggt gggaagagat tttcggagga tatgcctgaa tactggggcg actggtactg
     9601 tgactccgag gtgggcaagc tccgggccgt gctgatgcat cgcccgggtc cggaaataga
     9661 cgctgtcacc gacccggcat cggtgcgttt caaggccctt atggacccgg aaaaagcacg
     9721 ggaacagcat gatgcgctgg ccgggatcta ccgtgaccac ggagtcaggg tgtattacgt
     9781 agaagagggg cgtttagata aacctaacgc tcttttcatg agagaccagg tcctgatgac
     9841 cccggaaggg gccattgtct gccgtccggc catggaggtc cgtcggggtg aagagcgctt
     9901 cgccgcggaa acccttgccc gcctgggcgt cccgatcttg aagaccattt cgggcgaggg
     9961 ggtttttgag ggagcttgcc tcctttgggt ggacaggaag acggtgatcc tgggaaccgg
    10021 ttgccgggcg aacaaggccg gcgccctcca ggttgaaagg gaactgcgcg accttggcgt
    10081 ggatacaatc ctttatttcc agatccctta cggacatgct catcttgacg gcctcatgaa
    10141 cttcgccgac aggaaaaccg ccatgatatt cccttcccaa gtaccttatg acgtctgcaa
    10201 gcctctcttg gaccagggtt tccgaataat agagaacccc tacgtcgatg aagtcgagtt
    10261 gacctcaggt atcaattttg tggcccttga gcctgggaag gtagtgatcc cttcgggtaa
    10321 tccccgtacc tgcgcccttc ttggggaagc cggggtggaa tgtgtcgagg tggatataac
    10381 ggaattgctc aaaggctggg gtgccattca ctgtatgaca gcgttcctga aaagggatcc
    10441 catcgaagcc taggggtcag gtctacactt ttgactaaat cgcggaattg ggcggaaaaa
    10501 gggaggc
//
LOCUS       DAOSRG010000028        10494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6184_length_10494_cov_9.148290, whole genome shotgun sequence.
ACCESSION   DAOSRG010000028 DAOSRG010000000
VERSION     DAOSRG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6184_length_10494_cov_9.148290"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLM30_02075"
     CDS             complement(<1..406)
                     /locus_tag="PLM30_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53989.1"
                     /translation="MTYPGSRMFIVPFFVTVLLLLMPLSAMASPATVSVGLSVGACKA
                     VFSAPGGQVSLKSASGGKASFRSGVSIEAVSPGVIRAGSVKLKLPVSISGNALMKWND
                     RPYRGHFKLTQQGGAFNVINVLGIEDYLKGVLK"
     gene            complement(439..1473)
                     /gene="ruvB"
                     /locus_tag="PLM30_02080"
     CDS             complement(439..1473)
                     /gene="ruvB"
                     /locus_tag="PLM30_02080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR53990.1"
                     /translation="MDSEGPEEEISGFRPARLDEFIGQNELKSKLEIYIQAARKRGEP
                     LDHCLFYGPPGLGKTTLAGIIANEMQGQLRVTSGPALERTGDLAAILSNLQSGDVLFI
                     DEIHRLSPAIEEVLYPGMEDFSLDIIVGKGPLARNIRLQIPPFTLIGATTRLGLLTSP
                     LRARFGIVEQLELYTAEDISQIVTRGSRLLSVAIKPDAAMAIARSARGTPRVALRLLR
                     RVRDVAEVRCAPGIDLVLAETALEMLGVDSEGMDERDRRYLRALIDLFGGGPVGLSTL
                     SAALNEESQTIEDIYEPFLIQKGLIERTPRGRRATVNGFSCLGRRPPREILNDQGFLE
                     LIEEENGEPN"
     gene            complement(1516..2118)
                     /gene="ruvA"
                     /locus_tag="PLM30_02085"
     CDS             complement(1516..2118)
                     /gene="ruvA"
                     /locus_tag="PLM30_02085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOR53991.1"
                     /translation="MTLLHSLEGVISGISPDGIDLEVGGLGFFLLCSKSVLRDAVSGQ
                     RMKVLTSLQVSESGPLLFGFSDEEERVLFQLLLKVRGIGPRLATTILRTLDLSDIINA
                     IATGNSHLLCQVPGVGKKTSERLCFELRQQIEHSGFKSLVSTVSPESADTVQFVYEAL
                     DSLGFTRSEARPALAKILARKDPESPEQLLRDALGELRKS"
     gene            complement(2115..2618)
                     /gene="ruvC"
                     /locus_tag="PLM30_02090"
     CDS             complement(2115..2618)
                     /gene="ruvC"
                     /locus_tag="PLM30_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOR53992.1"
                     /translation="MRRICLGIDPGLGRLGYGVAIEEKSIIRAGEFGCITTEPGTPVE
                     ARILQIHEGLCPLIERTNPELLSVERLFFGKNTTTAENVFQVRGVVLFLAARHGIPVV
                     QPKPSEVKLAVCGNGRAEKAQVQKMVFRILRLESVPRQDDAADALAIAVTGLAMSSFR
                     MKTKSGD"
     gene            complement(2624..3376)
                     /locus_tag="PLM30_02095"
     CDS             complement(2624..3376)
                     /locus_tag="PLM30_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR53993.1"
                     /translation="MSGHSKWANIKHRKSAQDAKRGKEFQKYIKAVMVASKEGGPDPA
                     MNNRLKSAIERARAANVPVDTIDKAIKKGSGDMEGVNYDEITYEGYGAGGVAVLVEVL
                     TDNKNRTASEMRFLFSRSGGSLGEAGCVSWIFQRTGVIRVSGKDLDEEQLLAACLEAG
                     ADDLEDDGEGYSVYCEPAAMSAVRGSLEEAGYVVEGFEIDNLPKNTVPVTGRQEAEKL
                     LRFLETLEEHDDVQNVYANFDIPDEIMEEIAG"
     gene            complement(3383..4159)
                     /gene="nadE"
                     /locus_tag="PLM30_02100"
     CDS             complement(3383..4159)
                     /gene="nadE"
                     /locus_tag="PLM30_02100"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOR53994.1"
                     /translation="MERGLMDFYRDPAFLVNIISSWLKEKILDSGRSGGIVGLSGGVD
                     SAVVAALLKRVCGDRMLAVMMPCHSAEEDHLDALKVVQAFDLPWIRVDLGRVYDDLLD
                     SLPGEIADDDGIHLSNIKPRLRMTTLYCLGQKKSLLVCGTANRAEMAMGYFTKHADSG
                     VDVLPLGDLLKGEVNQVAKELGVPPDIIKKPPSAGLWPGQTDEGEMGVSYSEIDRYLA
                     TGTASPGSLEVIEGAIERTSHKRVFPPACLIEKGSWVGQG"
     gene            complement(4211..5026)
                     /locus_tag="PLM30_02105"
     CDS             complement(4211..5026)
                     /locus_tag="PLM30_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOR53995.1"
                     /translation="MKELTVHIIQSDVLWGNPSGNIARMEKRLEEIPGGPSLVVLPEL
                     WTCSYDNAGLREHALSSPSALEMMSRVCADKGFFLVGGSLPWVESSGELYNRAFFIGD
                     SGEIMGSYDKAHLFPLLDEPLFFEAGKSPFLFDLMGIPSAIAICYDIRYPEFMRSLAL
                     TGAELIVVPAEWPTARIDHWETLLRARAIENQVFVVGCNRSGEGGGQVFGGRSLALGP
                     DGSTVTRCPDEGEMIASVSIEPTMVGKIRKKLPFTGGRNPLLYSPVTALDEKP"
     gene            5065..5472
                     /gene="nikR"
                     /locus_tag="PLM30_02110"
     CDS             5065..5472
                     /gene="nikR"
                     /locus_tag="PLM30_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOR53996.1"
                     /translation="MDKLARFSVSLPAPLLEKFDSWVETRGFRNRSEALRELIRRFIS
                     ESCWEDGEKTVFGTVTLTYDHHSGDSAREITSLQHDFGDLIICSTHIHADHHHCFESV
                     VFHGKASRVKEFIRQLSGLRDVSSSDPVITTIP"
     gene            complement(5529..5690)
                     /locus_tag="PLM30_02115"
     CDS             complement(5529..5690)
                     /locus_tag="PLM30_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR53997.1"
                     /translation="MAGKTTYRCASCGKEFDLAEEDQALRCPECRGRTLIVVKGNPRK
                     KKGKCAPAG"
     gene            complement(5754..6701)
                     /gene="argF"
                     /locus_tag="PLM30_02120"
     CDS             complement(5754..6701)
                     /gene="argF"
                     /locus_tag="PLM30_02120"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOR53998.1"
                     /translation="MPINLRGRSFLTLKDFTTGEIQYLLDLSVSLKEKKRMGIRGNTL
                     AGKSIALIFEKPSTRTRCAFTVACVDEGGHPEFLGKNDIQLGHKESVKDTAKVLGRMF
                     DGIQFRGFKQSVVEDLALYSGVPVWNGLTDQYHPTQILADFLTIQENFGKLKGLRLVY
                     VGDGRNNMANSLMIGSAKMGIHFAIAAPGDLFPEKDLVEECRVIASDTGATIEFFEDP
                     REAVSKADAIYTDVWASMGEEDKLAERVALLKPYQVNSDLLKATGNADCIFLHCLPAV
                     KGNEVTEEVFESRNSKVFDEAENRLHTIKAIMVSTIGDL"
     gene            complement(6747..7979)
                     /locus_tag="PLM30_02125"
     CDS             complement(6747..7979)
                     /locus_tag="PLM30_02125"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOR53999.1"
                     /translation="MANEGGSFSVSSEVGKLKRVMLHRPGKELERLTIDNKDELLFDD
                     ILWLEEAQKEHDTFADLLRDNGVEVVYFRNCLSHVLGEDSVRDPLLDEVLALEAIDIP
                     LVSALKGVLMEVSASELAEILIAGLTKMEALEILPPCRSLVLRAMGDRDFLIHPLPNL
                     YFQRDPFSFINSGVIISVMSFEARRKEPLYARYIFKNHPYFKGIEFLFGDHPRDTYPY
                     RIEGGDILVLSDSVVAIGISQRTAAGTLQIVGKRIAEKAGVKTVLAVDIPKSRAAMHL
                     DTVFTMVDHDAFTIYPEVKEYIRIWQLEYSPEGEITSVIEHQNLGDCLRKVLHLDVIR
                     FIETGGGDPVAAARDQWNDGTNTLAIAPGVVITYRRNVVSNRVLQANGIKVFEVKGAE
                     LGRGRGGPRCMSMPLTRD"
     gene            complement(8154..9008)
                     /gene="speB"
                     /locus_tag="PLM30_02130"
     CDS             complement(8154..9008)
                     /gene="speB"
                     /locus_tag="PLM30_02130"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOR54000.1"
                     /translation="MERFIASRRSMKGVTWILQGVPLDMTVSRFHGTSEGPRTIRRES
                     RHLETFSTVLLGDLQDVPFADVGDIELVPGDLHLSLETIKTVSSSFFRRGLKVATLGG
                     EHLATLPVLEAASEVYPGMAVIHLDAHADLRDRFRGTKKNHATVMRRVAERCLASPSL
                     LFQFGIRSASADEYRWGKDNTSFFPLELSAPLRDVISMLGRRPVYLSLDIDIVDPGEA
                     PGTGTPEPCGFSAWEVLQALTLMKDLCMIGFDIVEVSPPNDVNGITSVLAAGLTRECL
                     IMWGRGDS"
     gene            complement(9011..9859)
                     /gene="speE"
                     /locus_tag="PLM30_02135"
     CDS             complement(9011..9859)
                     /gene="speE"
                     /locus_tag="PLM30_02135"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOR54001.1"
                     /translation="MQKRHNDIWFTEYQTRHLRLGLSITKVLANIQTPFQHLLVVETD
                     EYGKLMALDGAIQVTEKDEFTYHEMLAHVVLTAHPNPRRVLIVGGGDGGSAREAVKHP
                     SVEEVVLVDIDQEVINASRQFFPALSSGLEDPRVTLLTMDALEYVRGKKGEFDVVIVD
                     STDPVDFAEGLFREPFYRDVHRALNGEGLIAAQTESPFTDPDILTGSALEMKRVFPIV
                     KVYWGAMPTYPTGMWTYLAGSKVHDPEELKSPLPGRTRYYSGRIHRASFVLPPFLEEL
                     LVEREK"
     gene            complement(9877..10347)
                     /locus_tag="PLM30_02140"
     CDS             complement(9877..10347)
                     /locus_tag="PLM30_02140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOR54002.1"
                     /translation="MLPVPELFTLVVGRGEGKKKLTAFDSALLDAGVGNMNLLRVSSV
                     LPPKCAFRTELKMPFGSLLPIAYGSITSDVPGETISAAIGVGLPEDPDSFGMIFEFSG
                     FCPGSEAAIKVEEMVREAFAMRSLCLKEVKVKAIDHVVKECGTVFAGCPLFFPF"
BASE COUNT         2154 a         3123 c         2931 g         2286 t
ORIGIN      
        1 tcttcaggac gcccttcagg taatcctcga tgcccagaac gttgatcacg ttgaaggccc
       61 caccttgttg ggtcagcttg aagtggccac ggtagggcct gtcattccat ttcatcaggg
      121 cgttgccgct gatgctcacg ggaagcttca gcttcacgga accggcacga ataacgcccg
      181 gggagacggc ttctatcgaa acgcccgacc ggaaggaagc cttgcctccc gaggcgctct
      241 tgagggagac ttggccaccg ggggctgaaa aaacggcttt acaggcacct acggacagcc
      301 ccaccgaaac ggtcgccggt gaagccatgg cggaaagagg cattagtagg agaagtacgg
      361 tgacgaaaaa aggcacgatg aacatccttg aaccggggta tgtcataagg gctttctcct
      421 ttcccctgga tctctctgtc agtttggttc cccgttctct tcctcgatca gttccaggaa
      481 gccctggtcg ttcaggatct ctctgggagg tctcctccca aggcaggaaa aaccatttac
      541 cgtggccctt ctaccccttg gggtcctttc aattaacccc ttctggataa gaaagggttc
      601 atagatatct tctatggtct gactttcctc gttaagggcc gccgaaaggg tggaaagccc
      661 taccgggcct cccccgaaca ggtctatcag ggcccgtagg tacctccggt cgcgctcgtc
      721 catgccttca gagtcgacgc ccagcatctc cagggcggtt tcggcgagca ccaggtctat
      781 ccccggggcg cacctgactt cggccacatc cctaaccctc cgcaaaagcc tgagtgccac
      841 ccggggggtg cccctggcgc ttctcgcgat cgccatggcg gcgtcaggtt ttatcgccac
      901 cgaaagaagc ctggagcccc tggtgacgat ctgggaaata tcctccgcgg tgtagagttc
      961 caattgttcg acaatgccga atcttgcccg aaggggagaa gtgagcaggc ccaggcgggt
     1021 cgtggcccca atgagcgtaa agggcggtat ctggagcctg atgttcctgg cgagaggccc
     1081 cttcccaacg atgatatcga gggaaaagtc ctccatcccc gggtagagga cttcttcaat
     1141 agccggcgag aggcggtgga tctcgtcgat aaaaaggaca tcccctgatt gaaggtttga
     1201 aaggatggca gccaggtcac cggtcctctc aagggcgggc cctgacgtga cgcggagctg
     1261 cccctgcatc tcgttggcga tgatgccggc aagggttgtc ttgccaagcc cgggggggcc
     1321 gtagaacagg caatggtcca gcggctcgcc tcttttccgg gcggcctgga tgtagatctc
     1381 cagtttgctt ttgagctcgt tctgcccaat gaactcgtct agcctcgccg ggcgaaaacc
     1441 tgaaatctct tcttccggcc cctcgctatc aataacgggg tagatattat cgacgggatc
     1501 cctcaaagga acacctcaac tttttctcaa ttcgcccagg gcgtcgcgca gcagctgctc
     1561 ggggctctcc ggatctttgc gcgccaatat cttcgccaag gcgggcctgg cctcgcttcg
     1621 agtgaagccc agggagtcga gggcctcgta aacgaactgg accgtatcgg ccgactcggg
     1681 ggagaccgtt gaaacgaggc tcttgaaacc ggagtgttcg atctgttgac ggagttcaaa
     1741 acagagcctc tccgatgtct tcttgcccac gccgggcacc tggcaaagga ggtggctgtt
     1801 tccggtggct atggcgttta ttatgtcgct taggtcgagg gtcctcaaga tcgtcgtagc
     1861 cagtcgggga ccgatgcccc tgaccttgag gagaagctgg aagagtaccc tctcttcttc
     1921 gtcgctgaaa ccgaacagca agggacccga ctcgctgacc tggagggatg tgaggacttt
     1981 catcctttga cccgagacgg cgtccctcaa cacgctcttg gagcagagga ggaaaaagcc
     2041 cagaccgccc acttccaggt caataccatc gggcgatatg ccggaaataa cgccttcaag
     2101 gctgtggagc aaggtcaatc accactcttc gtcttcatcc ggaaactcga cattgccagt
     2161 ccggtgactg cgatggccag ggcgtcggcc gcgtcgtcct ggcgggggac gctctcaagt
     2221 cgcaggatcc ggaagaccat tttctggacc tgtgcctttt ccgccctgcc attcccgcag
     2281 acggccagtt ttacctccga tggcttcggc tggaccacag ggatcccgtg cctcgcggcg
     2341 aggaaaagca ccacgcccct gacttggaag acattctccg ctgtcgtggt attttttccg
     2401 aaaaaaaggc gttccaccga gagcaactcg gggttggtcc gctcgatcaa ggggcacaac
     2461 ccttcatgga tctggaggat ccgcgcctcg accggggtcc cgggttccgt cgtgatgcac
     2521 ccgaactcgc cggccctaat gatgctcttt tcctctatgg caaccccgta accgagcctt
     2581 ccaaggccgg gatctatccc gagacagata cgtcgcatcc cgatcagccg gctatctctt
     2641 ccatgatctc atcgggtata tcgaaattag cgtagacatt ctgcacgtcg tcatgttcct
     2701 caagggtttc caggaagcgg agcagttttt ccgcctcctg tctccccgtc accggcaccg
     2761 tgttcttcgg aaggttatcg atctcgaaac cttcgaccac gtatccggcc tcctcgagag
     2821 atcccctgac cgcggacatg gcggcgggct cgcagtaaac ggagtaaccc tccccgtcgt
     2881 cctcgaggtc gtccgccccg gcttccaggc acgccgccag gagttgctcc tcatccaggt
     2941 ccttccccga taccctgatc acaccggtgc gttggaagat ccaggagacg caccccgctt
     3001 cccccaggga gccgccggac cggctgaaaa ggaagcgcat ctccgaggcg gtcctgttct
     3061 tgttgtccgt cagcacttcc accaggacag cgaccccacc ggccccgtat ccttcgtagg
     3121 tgatctcgtc atagttgacg ccctccatgt caccggagcc tttctttatc gccttgtcaa
     3181 tggtatccac cggcacgttg gccgccctgg cgcgctcgat ggcggattta aggcggttgt
     3241 tcatggccgg atcgggaccg ccctctttgg atgcgaccat cacggccttg atgtacttct
     3301 ggaactcctt tcccctcttt gcgtcctggg cagatttcct atgcttgata ttcgcccatt
     3361 tcgaatgacc tgacattgtt tttcacccct gtccaaccca actacccttc tcgatcaggc
     3421 aggccggcgg gaaaaccctc ttgtgcgagg ttcgctcgat agccccttcg atcacttcga
     3481 ggctgccggg cgatgccgtg ccggtagcca agtacctgtc gatctcggaa taactgaccc
     3541 ccatctcgcc ctcgtcggtc tggcccggcc agagtccggc tgagggaggt ttttttatga
     3601 tatccggagg caccccgagt tcttttgcta cctggttgac ctcacccttg agaagatcgc
     3661 ccagggggag cacgtccacc ccggaatcgg cgtgctttgt gaaataaccc atggccattt
     3721 ccgccctgtt ggccgttcca cacacgagaa ggctcttttt ctggcccagg caataaaggg
     3781 tggtcatcct gagcctgggt ttgatattgg agaggtggat cccgtcatca tcggctattt
     3841 caccggggag cgaatccagg aggtcgtcgt aaaccctgcc aagatcaacc ctgatccagg
     3901 gaagatcgaa ggcttgcacc accttcaggg catccaggtg gtcttcctcc gcgctgtgac
     3961 aaggcatcat taccgcgagc atcctgtcgc cgcagaccct cttgaggagg gcagcgacca
     4021 cggccgaatc aacaccgccg ctcaacccga ctatccctcc ggatctgccg gagtcgagga
     4081 tcttttcctt cagccaggaa gagatgatat tcacgaggaa agcaggatca cggtaaaaat
     4141 ccatgaggcc acgctccata agatctattt tgaagggtca tcccaagaat aattctagca
     4201 caggctttcc tcagggcttt tcatccaggg cggtgacagg ggaataaaga aggggattcc
     4261 tacctcccgt gaaaggaagc tttttcctga tcttgccaac catggtcggc tcgatggata
     4321 cgctggcgat catctcccct tcgtcgggac agcgagtgac cgtggagcca tcggggccaa
     4381 gggcgaggga cctcccgccg aagacttggc ctcccccttc cccactccgg ttgcagccga
     4441 ccacgaagac ctggttttcg atcgccctgg ctcggaggag ggtctcccag tggtcgatcc
     4501 tagccgtggg ccattcggcc gggaccacga tcagttcggc ccccgtcagt gccagggacc
     4561 gcatgaattc gggatacctt atgtcataac aaatggcgat cgccgacggg atacccatga
     4621 ggtcgaaaag aaaggggctc ttcccggcct cgaaaaacag gggttcatcc agcagtggga
     4681 ataagtgggc cttgtcatag gatcccatga tctctcccga atctcctatg aagaaggccc
     4741 ggttgtacaa ttcgccggag gactccaccc agggcagact tccgccgacg aggaagaacc
     4801 ccttgtcggc gcataccctg gacatcattt caagggccga gggactggac agggcatgct
     4861 cccttaaccc ggcgttgtcg taggagcagg tccacagttc gggcagcact accagcgagg
     4921 gtcccccggg gatctcttca aggcgcttct ccatcctggc gatattgcca gaagggttgc
     4981 cccacaggac atcagactgg ataatatgga cggtgagttc tttcaaggcc cttcccccct
     5041 cgatcctcgc tcggaggtga tattttggac aaactggcaa ggttcagcgt ctccctgccc
     5101 gcgcctcttt tggaaaaatt cgattcctgg gtcgagacgc ggggtttccg gaacaggtca
     5161 gaagccctga gagagctgat ccggcgtttt atctccgaat catgctggga agatggcgag
     5221 aaaaccgtct tcgggaccgt cactctgacc tacgatcatc actccggtga ttcggcccgg
     5281 gaaataacca gcctgcagca tgatttcggg gacctcataa tctgctcgac ccatatccat
     5341 gccgaccatc accattgttt cgagagcgtc gtttttcacg gcaaggcgtc cagggtcaag
     5401 gaattcatcc ggcaattgag cgggttaagg gatgtctcca gttccgaccc ggtcattacg
     5461 acgatcccct gaagcgggaa accagggtcg gaggccctta tcggtgacga ccgtcaccgt
     5521 cagatgcctc acccggcggg ggcgcatttc cccttctttt tcctggggtt gcccttgacg
     5581 acgatgagcg tacgcccgcg acactcgggg caacgaagcg cctgatcttc ctcggccaag
     5641 tcgaactcct tgccacagga ggcgcatcgg taagtggtct ttccagccat atctctcact
     5701 tcctccaaat gggtctttcc tgttcgtctt tcgcgggatc ttaggagcgc ggtctaaaga
     5761 tcccctatag tggacaccat aatcgccttt atcgtatgaa gcctgttctc agcctcatcg
     5821 aacaccttgg agttcctgga ttcgaagact tcctccgtca cctcgttccc cttcacggcc
     5881 ggcaggcaat gaaggaagat gcagtcggca ttccccgtgg ctttcagaag gtcggaattg
     5941 acctggtaag gcttcaacag ggccaccctt tcggcgagct tgtcctcttc ccccatcgag
     6001 gcccaaacgt cggtgtagat cgcgtcggcc ttgctgacgg cctcccgcgg gtcctcgaag
     6061 aattcgatcg tggcccccgt atcggaggcg atcaccctgc attcttccac gaggtccttt
     6121 tcggggaaga ggtctcccgg ggcggcgatc gcgaagtgga tccccatctt ggccgaaccg
     6181 atcatcaggg aattcgccat attattcctt ccatccccaa cgtagaccag cctgagccct
     6241 ttcagttttc cgaaattctc ctgtatcgtc aggaaatcgg cgaggatctg ggtggggtga
     6301 tactggtcgg tgaggccgtt ccagacggga acccctgagt aaagggccag gtcttcaaca
     6361 acggactgtt tgaaaccgcg aaactgtatc ccgtcgaaca tcctgcccag gaccttggcg
     6421 gtgtccttga cagactcctt gtgacccagc tgaatatcgt tctttcccag gaattccggg
     6481 tggccgcctt cgtcgacgca ggccacggta aaggcgcacc tcgtcctggt ggagggcttc
     6541 tcgaagatca gggcgatact ctttcctgca agggtgttgc cccttatccc catcctcttt
     6601 ttctccttca gcgagaccga aaggtcgagc aggtactgga tctccccggt ggtgaaatcc
     6661 ttaagggtga gaaaacttct tcctctcagg ttgatcggca ttccattccc ctcctgtttt
     6721 cttgtaagtt gaagcttcgg ccttggctaa tcccgggtca ggggcatgct catgcacctg
     6781 gggccccccc ggcccctgcc gagttcggcg cccttcacct caaagacctt tataccgttg
     6841 gcctggagca ccctattgga gacgacattc cttctataag taatgacgac cccgggagcg
     6901 atagccaggg tgttggtccc gtcattccac tggtcccttg ccgcggcgac ggggtccccg
     6961 cccccggtct caatgaaacg gatcacatca agatgaagca ctttccgcag gcaatccccc
     7021 aagttctggt gttcaatgac agaggtgatc tccccctcgg gggagtactc gagttgccat
     7081 atcctgatat attccttcac ttccgggtaa atggtgaagg cgtcatggtc caccatggtg
     7141 aagactgtgt caaggtgcat ggccgcccga ctcttgggga tgtctaccgc caaaacggtc
     7201 ttgaccccgg ctttttcagc gatcctcttg ccgacgatct gcagggtccc cgccgcggtt
     7261 ctttggctga tcccgatggc cactacggag tccgagagga caaggatatc ccccccttcg
     7321 atgcgataag ggtaggtatc gcgggggtgg tccccgaaga gaaattctat acccttgaaa
     7381 taggggtggt tcttgaatat atagcgggcg taaagtggtt ccttcctccg ggcctcgaag
     7441 gacatgacgc tgattattac gcccgaattg atgaatgaga agggatccct ctggaaatag
     7501 aggttgggga gagggtggat caggaaatcc cggtcgccca tcgctctcag gacaagactc
     7561 ctgcaggggg ggaggatctc cagggcttcc atttttgtga ggccggcgat gagtatttcg
     7621 gcgagttcgc tggcgctcac ttccatgagt acacccttga gggccgagac cagggggatg
     7681 tcgatagcct caagggcgag cacttcgtcg aggagcgggt ccctgacaga gtcttcacca
     7741 agcacatggg aaagacaatt tctgaagtaa accacctcca ccccgttatc ccgaaggagg
     7801 tcggcaaagg tgtcgtgctc cttttgggcc tcttcaagcc agagtatgtc gtcgaagaga
     7861 agttcgtcct tgttgtcgat ggtgaggcgt tccagttcct tgccgggcct gtgaagcatg
     7921 acccttttca gtttaccgac ttccgacgat acgctgaaag aaccgccctc attggccacg
     7981 aaagatacct ccccgtctct ttgaatttgc cccgaggatt agtataagcc atcgacggta
     8041 gaaaaacggg aacgggacca ttcccctgac gcccatggac ctctaagagg ctcccccggg
     8101 gcggccggtc cgtcccgcgt cagagtgcgg gaacgcggcc aagggcgagg ctcttaggag
     8161 tcgccccttc cccacatgat caggcattcc cgggtcagcc cggccgccag gacggaggtt
     8221 attccgttaa cgtcgttggg ggggctcacc tctacgatgt cgaagccgat catgcagagg
     8281 tccttcatga gagtcagggc ttgaagaacc tcccaggccg aaaaaccgca aggctccggg
     8341 gtgcctgtgc cgggagcctc ccccgggtca acgatatcga tatcgaggga aaggtagacc
     8401 ggcctccgcc ccagcatgga aatgacatcc ctcaaggggg ccgacagttc gagggggaag
     8461 aaggaggtgt tatcctttcc ccaccggtac tcatcggccg acgcggacct tatgccgaac
     8521 tggaaaagaa gggaagggct ggcgaggcat ctctccgcaa cacggcgcat gacggtggca
     8581 tgattttttt tcgtcccccg gaagcgatcc ctcaagtccg cgtgggcatc gaggtgaatg
     8641 accgccatac cggggtaaac ctccgaagcg gcctccaaca cggggagtgt cgcaaggtgt
     8701 tcgccgccca gagtagcgac cttgaggcct ctacggaaaa aacttgagga aaccgtcttg
     8761 atcgtttcaa gggaaaggtg caggtcgccg gggacgagct cgatgtcccc gacatccgcg
     8821 aagggaacgt cctgaaggtc cccgagaagt actgtactga atgtttccag gtgccgtgat
     8881 tccctcctta tggtacgagg cccctccgag gtcccgtgaa agcgggatac ggtcatgtcg
     8941 agggggactc cctggaggat ccacgtcaca cctttcatgg atcttcggct cgctatgaac
     9001 ctttccaagg ttatttttcc cgttcgacca ggagttcttc caggaaagga gggagaacaa
     9061 aagaggccct gtggatccgg ccggaatagt acctggtcct cccggggagg ggactcttca
     9121 gttcttccgg gtcatggacc tttgaccccg ccaggtaggt ccacatcccc gtcggatagg
     9181 tgggcatcgc tccccagtaa accttgacga tggggaagac ccgtttcatt tccaaggcgg
     9241 accccgtcag tatgtcagga tcagtaaagg gagattcggt ctgggccgct atgagacctt
     9301 ccccattgag cgctcgatgg acgtccctgt aaaagggttc acggaaaaga ccttcagcga
     9361 aatcgacggg gtcggtgctg tccacgataa cgacgtcgaa ttcacccttt ttccccctga
     9421 catactcaag agcgtccatc gtcagaaggg tgaccctggg atcttccagg ccggagctca
     9481 atgcggggaa gaattgcctc gaggcgttta tgacctcctg gtcaatatcg accaggacca
     9541 cttcctcgac ggaagggtgt ttgaccgctt ccctggcgct ccccccatcc ccgccaccaa
     9601 cgatgaggac ccgtcgcggg ttcgggtggg ccgtgaggac gacgtgggca agcatttcat
     9661 ggtaagtgaa ttcatccttt tcagtaacct gtatcgcgcc gtctagggcc atcagcttcc
     9721 catactcgtc tgtttccacc acgaggaggt gctggaaagg agtctggata ttggccagga
     9781 ccttggttat gctcaagccc agccttaaat gccttgtctg gtactccgtg aaccagatgt
     9841 cattgtgtct cttttgcatc gccctaggcc cttcccctaa aagggaaaga aaagcgggca
     9901 accggcgaac acggtcccgc attcttttac tacgtgatcg atcgccttga cctttacttc
     9961 tttgagacac agtgagcgca ttgcaaaggc ttccctcacc atctcctcga cctttatcgc
    10021 ggcttccgac cctgggcaaa atcctgagaa ttcgaagatc atcccaaaag aatcggggtc
    10081 ctccggaagg ccaaccccta tcgcggcgga gatggtctca ccgggaacat cgctcgtgat
    10141 cgaaccatag gcgataggca gaagtgagcc gaagggcatc ttcagttccg ttctgaaagc
    10201 gcatttcggc gggaggacgc tgctcacgcg taggagattc atattaccga ccccggcatc
    10261 cagaagagct gaatcgaagg ccgtcagttt ctttttcccc tcaccgcggc ccaccacgag
    10321 cgtgaaaagt tcaggcacgg gcaacattgc aaggcaacct cccctcgatg gtcattcccc
    10381 aaaaaagcgc atatcattat atcgtcagaa gggggggtgt caattgagcg ggtcagggag
    10441 tgtcgccgga cagggataat gtcacccctt aaggggacag ggaaaatgtc accc
//
LOCUS       DAOSRG010000029        10115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6455_length_10115_cov_9.180915, whole genome shotgun sequence.
ACCESSION   DAOSRG010000029 DAOSRG010000000
VERSION     DAOSRG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_10115_cov_9.180915"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLM30_02145"
     CDS             complement(<1..959)
                     /locus_tag="PLM30_02145"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOR54003.1"
                     /translation="MLLGNAVLFDGTRFLAGEKDVAVEDGVITTVGKVSRTGEFDVIK
                     DLKGKILCPGFIDLHCHLRDPGQEWREDLESGARAGAAGGFTTLVCMPNTDPPLDTPA
                     LVRYVLEKNEKCHGSRVLPSGCVSKERKGKTMAEMGKMARAGAVIFTDDGSPIVDTGL
                     FRLALQYSTWLGVRIMEHPEEKSLTAGAQVNEGICSTFMGLKGWPSSAEASDISRGCS
                     LSRDTGAALHFTHVSAEDPISVLREAKAAGLPVSCDVTPHHLCLTEEEILAGGADSVF
                     KVNPPLRSNRDRDALWEALVDGTVDAIATDHAPYHLDEKDLPF"
     gene            complement(962..1891)
                     /locus_tag="PLM30_02150"
     CDS             complement(962..1891)
                     /locus_tag="PLM30_02150"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOR54004.1"
                     /translation="MEWKQRHILDLDTWDRSELQYILEQTRSMDELLDRPIKKVPALR
                     GKLVVNLFFEPSTRTRVSFELAEKFLSADVVNWSAPNSSTEKGETLRDTAWTLEAMGA
                     DAVVIRHGAVGAADYLARKLKKSVVFNGGDGAHAHPTQALLDLYTAWRKLGVLEGRKM
                     AIIGDVLHSRVARSNIIAFNRMGVRVVLSGPPTLMPKIPEALGATFTKDPVDAVRGAD
                     IVYLLRIQRERQIEGMFPSLDEYHQFFGVDDRLLGSASPDALVMHPGPINRRVEIASE
                     VADGPRSMILGQVRSGVALRMALLFVCLGGVRQ"
     gene            complement(1895..2440)
                     /gene="pyrR"
                     /locus_tag="PLM30_02155"
     CDS             complement(1895..2440)
                     /gene="pyrR"
                     /locus_tag="PLM30_02155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOR54005.1"
                     /translation="MKEKAKVMNEKDMERALKRMSHEILEKNQGLEDMVLVGIQRRGV
                     YLANRLRDIFFSLESFKIPAGVLDITLYRDDLTLLHDQPVVHSTAIPEDLNARKILLV
                     DDVLYTGRTIRAALDALMDLGRPSCVQLAVLIDRGHRELPIHPDFVGRNVPTSRNETV
                     EVRVKELDGLDEVVICEREGF"
     gene            complement(2619..3338)
                     /gene="rnc"
                     /locus_tag="PLM30_02160"
     CDS             complement(2619..3338)
                     /gene="rnc"
                     /locus_tag="PLM30_02160"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOR54006.1"
                     /translation="MKHSSPGCYAAEEEHRLKELEERLDYHFRDGLLLREALTHASFS
                     NENGLEYCNERLEFLGDAVLELCVSETLFKRFPEASEGELSARRASLVCEDSLSEWAV
                     KVGIPESLRLGRGLCKSGGRQQPSLSADAVEAVFGAVLIDGGYEAARAMIEKYLEDSE
                     SFERGVPRNPKADLQELMEKRGLGNPEYLLIGQAGPPHAPVFRVIVKQLGDAPGTGEG
                     RTIREAERKAAEIGLQFLLRQ"
     gene            complement(3340..4581)
                     /gene="fabF"
                     /locus_tag="PLM30_02165"
     CDS             complement(3340..4581)
                     /gene="fabF"
                     /locus_tag="PLM30_02165"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOR54007.1"
                     /translation="MRRVVITGMGVISPIANGREEYWKSLKEGRNGVGGITLFDPADF
                     AVRIAAEVQDFDPERWMDRKEARRADRVIQFAVGASDMAVNDASLDVDCLDPDRFGVF
                     IGSGEGGISTMYEGMKVLMEKGPSRVGPFCVPMMLSNMPAAYVAIRFGAKGPNICVVT
                     ACATATHTMGEAFHTIVRDDADVILAGGTEAAITPVAVAGFAAMKALSTRNEDPLHAS
                     RPFDIDRDGFVMGEGAGVLVFEELEHARKRGARIFAEVKGFGSTCDAYHITAPDPQGE
                     GPAKAMVQAVKKAGWGIDEVDLINAHGTSTILNDTMESSAINRAFGEFTGKILVHSTK
                     SMIGHTLGAAGALESIAALQAIVEGIVHPTTNCVNPDPECRINVIPDGNPLKREVSRV
                     LVNNFGFGGHNAVMAVERFQE"
     gene            complement(4686..4937)
                     /gene="acpP"
                     /locus_tag="PLM30_02170"
     CDS             complement(4686..4937)
                     /gene="acpP"
                     /locus_tag="PLM30_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR54008.1"
                     /translation="MKMDEITARLKEIVMDRLDVEEDQIKPEASFVEDFGADSLDIVE
                     LIMGIEEVFDIEIPDEDAEKLTNVGEAMEYIKGKLGVEE"
     gene            complement(4975..5721)
                     /gene="fabG"
                     /locus_tag="PLM30_02175"
     CDS             complement(4975..5721)
                     /gene="fabG"
                     /locus_tag="PLM30_02175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOR54009.1"
                     /translation="MNPEKRVALVTGSGRGIGRAIALAMAELGHTVAVNYRSSSVAAE
                     RLVEEILSFGGRARAFQADVSSASEVERLFEEAESDLGPVGILINNAGITRDGLLMRM
                     KDPDWEDVIRTNLLSNFLTTRRAAKDMVRSRWGRIVNISSVVALIGNPGQANYAAAKA
                     GVIGLTKSAARELGSRGITVNAIAPGFIETDMTAVLDEKIRSQMMLQIPAGRAGRPEE
                     VAALVRFLVSEEASYITGQVIAVDGGWTMA"
     gene            complement(5711..6670)
                     /gene="fabD"
                     /locus_tag="PLM30_02180"
     CDS             complement(5711..6670)
                     /gene="fabD"
                     /locus_tag="PLM30_02180"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOR54010.1"
                     /translation="MKYAVVFPGQGAQEVGMGKGFFDRYAASRAVFERSDDALGFNLS
                     RIIFFGPDDELKKTEYAQPAILAMSMAVFRCLEEEKGVVFSPEYVAGHSLGEYTALVA
                     SEALSLEDGLRLVHLRGKLMQEAVPLGVGSMAAVIGLDRESVISICESVSTLGVCQAA
                     NFNAPGQIVISGEKGAVDKALALCKDAGAKRALPLKVSAPFHSCLMRPVADQLRESFA
                     QCEWKRPKWPLVANVSAQEETDVGSIQEALFLQTFMPVLWSGSVEFIISRGVRHFVEI
                     GPGSVLTGLIKRISSDANCFSLFDEDGLDEVIDFLRGGQYDES"
     gene            complement(6690..7640)
                     /gene="fabK"
                     /locus_tag="PLM30_02185"
     CDS             complement(6690..7640)
                     /gene="fabK"
                     /locus_tag="PLM30_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOR54011.1"
                     /translation="MFINNRITRLLGVVYPVIQGGMAWVADADLAAAVSNGGGLGVIA
                     AGSMPPDLLESQIAKVRALTNKPFGVNIMLLSATADAALEVVERNRVPVVTTGAGAPG
                     KVIERLKPLGTKVIPVIASVAHAKRVEKQGADAVIAEGMEAGGHIGEITTMVLVPQIV
                     DAVNIPVVAAGGVAEPRGVLAAFSLGAEGVQVGTRFVCACESNAHEAFKNEIIKARDR
                     STAITGASTGHPVRCIQNRLTREFARLEKAGASVEELEKLGTGRLRAAVVEGDVEFGS
                     VMAGQSAALVNRIQPAAEMIRELFEGTQEMFESLHRFSNH"
     gene            complement(7651..8646)
                     /locus_tag="PLM30_02190"
     CDS             complement(7651..8646)
                     /locus_tag="PLM30_02190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOR54012.1"
                     /translation="MNFNRCAPVVLLGTGMSVPTRVVTNRDLEKMVETSDEWIVERTG
                     IRERRIASMGELTSDLAAAAAMEALDAASVLPEELDMIIVGTNSPDRLFPGVGPIVQA
                     RIGASKAGACDLQAGCTGCVYGLMLASAGIRAGFWKKVLVIGAEVLSRIVDWKDRNTC
                     VLFGDGAGAALLGAGEGEDGVLAAEVHADGNLDDYIELPGGLVAHPATVETVEQGLHF
                     VKMKGNEVFKYVNKVIPPFVRDLCSQAGLDVREIDAWVFHQANHRIIEGVLRRLEIPL
                     DKAVVNLQKYGNTSAASVFLALHEGMAEGKISKGDKVMLVSFGAGMTYGAMIIRI"
     gene            complement(8643..9650)
                     /gene="plsX"
                     /locus_tag="PLM30_02195"
     CDS             complement(8643..9650)
                     /gene="plsX"
                     /locus_tag="PLM30_02195"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOR54013.1"
                     /translation="MLLALEAMGGDNAPGETCAGAIMACEKFTDLEIALVGDSEAIAP
                     LLMNTSAPVRKRLHLVHAEEKIAMDEPPAVAVRRKRGSSLRIAMQMVRSGEAQGCVSA
                     GSTGAIVAAGVLVVGRIPGIERPGLGVPLPAVNRISMLIDIGATVRCKPLNLFQFALM
                     GDVYMRKIIGVDSPSIGLLSNGQEEAKGDELIIEAREYFKNGRLNFIGNVEGGGIPFG
                     AADVVVCEGLTGNILLKFIEGTGEAIYSLIREEIDRRILAKMGMVFMIPMLKDLWARF
                     NYEETGGTPLLGVNGAVIKAHGRSRARAICGAIRVAREFVNKRGVQTIAEELAEGVKR
                     G"
     gene            complement(9634..>10115)
                     /locus_tag="PLM30_02200"
     CDS             complement(9634..>10115)
                     /locus_tag="PLM30_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOR54014.1"
                     /translation="AMLGIPEVRERMRSMANQAGSRLRSLKREEVIGELLDLQPGEWA
                     LSTLQVSREMAFRHTDYASDHYIYAMASSLAIAVIEADMVVTGSARVRFRFPAKVGER
                     LVARAKVGTKKGNKNVVSVRIRVGEQEIFVGRFITVEKECQDGTPAQGRGEASVAGS"
BASE COUNT         2011 a         3244 c         2590 g         2270 t
ORIGIN      
        1 tgaaagggca agtccttttc gtccagatga tagggcgcat ggtctgtcgc gatcgcatcc
       61 actgtcccgt caaccagggc ttcccagagg gcatccctgt ccctgttcga cctgaggggg
      121 gggttgacct tgaagacgct gtccgccccc cctgctaata tctcttcctc ggtaaggcaa
      181 aggtgatggg gtgtaacatc gcagctgacg ggtaatcccg cggctttggc ctccctcagt
      241 accgagatgg gatcttcggc gcttacgtga gtgaagtgca gagccgcccc ggtatccctg
      301 gaaagggagc aacccctgga aatatccgac gcctcagcgg aagaaggcca ccccttgagc
      361 cccatgaagg tggaacagat cccttcgttc acctgggctc ctgccgtcag gctcttttcc
      421 tcagggtgtt ccatgatcct gaccccgagc caggtgctgt attgcaaggc aagcctgaag
      481 agaccggtgt cgacgatggg gctaccgtca tcggtgaaga tcacggctcc cgccctggcc
      541 atcttgccca tctcggccat ggttttaccc ttcctctcct tgcttacgca acctgagggg
      601 agtactctcg atccgtgaca cttttcattt ttttccagga catacctcac tagggcgggg
      661 gtgtccagag gcgggtcagt gttgggcatg caaacaaggg tggtaaatcc ccccgcggct
      721 ccggccctgg ctccggattc aaggtcctcc ctccattcct ggcctggatc cctgaggtgg
      781 caatggaggt cgatgaaacc cgggcagagt atcttcccct tcagatcttt gataacatca
      841 aactccccgg ttcggctgac cttaccgacg gtggtgataa cgccgtcttc tacggcgaca
      901 tccttttcac ccgccaggaa ccgtgtccca tcgaagagta ccgcattacc caacagcatt
      961 ttcactgtct cacacctccg aggcaaacga agaggagtgc catcctgaga gccactccgc
     1021 tgcggacttg tcccaggatc atgctccgtg gaccatcggc tacttcgctc gcgatctcga
     1081 ctcgcctgtt tatcggaccg gggtgcatca ccagggcgtc aggggatgcc gatcctagaa
     1141 gcctgtcatc gacgccgaaa aactggtgat actcatccag ggaggggaac atgccctcga
     1201 tctgtctctc acgctggatc ctcaacaggt agactatatc cgcaccccgg acggcatcca
     1261 ccgggtcctt tgtgaaggtt gccccaagag cttcagggat ctttggcatg agtgtcgggg
     1321 gaccggacag tacgacccgg acacccatgc ggttgaaggc gatgatattg cttctcgcca
     1381 ctcggctgtg gagtacgtcc ccgataatgg ccattttgcg gccctcgagg acgcccagtt
     1441 ttctccaggc ggtgtaaaga tccaggagcg cttgtgtcgg gtgtgcgtga gccccatcac
     1501 ccccgttgaa gacgacgctc tttttcaatt tcctcgccag gtaatccgcc gccccgacag
     1561 ccccatgtct tatcacgacc gcatccgccc ccattgcttc cagggtccat gccgtgtccc
     1621 ggagggtctc ccccttttca gtgcttgaat tgggcgcact ccagttgacc acatcggcgc
     1681 tcaggaactt ctccgccaat tcgaaggaaa cccttgtcct ggtggaaggc tcgaaaaaaa
     1741 ggttgaccac cagcttgccc cgtagagccg ggaccttctt gatgggcctg tccagcaact
     1801 cgtccatgga ccgggtctgt tcaagtatgt actgcaattc ggagcggtcc caggtatcaa
     1861 gatcgaggat gtggcgttgt ttccactcca ttcgtcaaaa accctccctc tcgcagatga
     1921 cgacttcatc cagcccgtcc agttctttta cgcggacctc gaccgtctcg ttcctggacg
     1981 tggggacgtt acggccaacg aaatcggggt ggatggggag ttcccggtgg cccctgtcga
     2041 tgaggacggc aagttgcacg caagagggcc tgccaaggtc catcagggca tcgagggccg
     2101 ccctgatcgt tcggcccgtg tacagcacat catcgaccag gaggatcttc ctggcattca
     2161 ggtcctcggg gatagcggtg ctatggacca ccggctggtc atgtagaagg gtcaggtcgt
     2221 ccctgtaaag ggtgatatcg agaacaccag ccgggatctt gaaggattcc aggctgaaaa
     2281 aaatatccct gagacgattc gcaaggtaga cgcctctcct ctggatccct acgaggacca
     2341 tatcttcaag cccctggttt ttttccagta tctcatggga catcctcttg agagcccttt
     2401 ccatgtcctt ctcgttcatt accttcgctt tttctttcag cacaggaaca cctccttaaa
     2461 aaaaaaagag cgcggttccc tcttcttgag ggtccgagcc cctttctttt tcgccttcct
     2521 gagatccctt tgaccggatg atttttcttt ccttgctcaa acgtacaccc ccacaaaggg
     2581 attttaccag gggggggaat ataatgcaac cggttgcatt attgacggag gaggaattgc
     2641 aacccgatct cggcagcctt cctttcggct tcccttatgg tccttccctc acctgtaccg
     2701 ggtgcgtcac ccagctgttt cacgatcacc ctgaataccg gcgcatgggg agggccggcc
     2761 tgcccaatca acaggtattc tgggttgccc agtccccttt tttccatcag ctcctgcagg
     2821 tctgccttgg ggttcctggg gacacccctt tcgaagcttt cgctatcctc aaggtacttc
     2881 tcgatcattg ccctggccgc ctcgtacccc ccgtcgataa ggacagcccc gaatacagcc
     2941 tcgacagcat cagcgctgag agagggctgt tggcgcccgc cgcttttgca aaggcccctg
     3001 cccagcctga ggctttcggg gataccaacc ttgacggccc actcgctcaa tgaatcctcg
     3061 caaaccagcg aggcccttct tgctgacaac tcgccctcgc tcgcctcggg gaatcttttg
     3121 aaaagcgttt ccgacacgca cagttccaaa accgcgtcgc cgaggaactc aagcctttca
     3181 ttgcagtatt ccaggccgtt ttcattggag aaggaggcgt gggtcaaggc ctccctcaac
     3241 aaaagtccgt ccctgaagtg ataatcgagc cgctcctcca gttctttcag ccggtgttct
     3301 tcttccgccg cgtagcagcc aggtgaagaa tgtttcactt tattcctgga acctctctac
     3361 agccatgaca gcattgtggc ccccgaagcc gaagttgttc acgagcaccc gcgacacttc
     3421 tcttttcaag ggattcccgt cgggtataac gttgatccgg cattccgggt cagggttgac
     3481 acagttcgta gtaggatgca cgatcccctc aactatggcc tggagtgcag caatggactc
     3541 cagcgccccc gccgccccaa gggtatgacc tatcatggat ttggtcgaat ggacgaggat
     3601 ctttccggta aattccccga aagccctgtt gatggccgac gattccatgg tgtcgttgag
     3661 gatggtcgat gtaccgtggg cattgatgag gtctacttcg tcaattcccc aaccggcttt
     3721 tttgaccgcc tggaccatgg ccttggccgg gccctcccct tgagggtccg gggccgtaat
     3781 gtgataggcg tcgcaagttg acccaaaacc cttcacctcg gcgaatatcc tggctcccct
     3841 cttcctggcg tgttccagct cttcgaacac cagaactccg gcgccttcgc ccatgacgaa
     3901 accgtccctg tcgatatcga agggccggga ggcgtgaaga gggtcctcat tccttgtcga
     3961 gagggctttc attgcggcaa accctgcgac ggcaacagga gttatcgcgg cctcggtgcc
     4021 cccggcaagt attacatccg catcatctct tacgatcgtg tggaaagcct cgcccatcgt
     4081 atgggttgcc gtggcgcagg ctgtaacgac acagatgttc ggtcccttgg ccccgaaacg
     4141 gatcgccacg taggctgcgg gcatattgct cagcatcatg ggaacacaga aagggcctac
     4201 cctggatggc cccttctcca tcagcacctt cataccctcg tacatagtgg aaatgccgcc
     4261 ctcgccgctc ccaatgaaga cgccgaatct atccggatcc aggcagtcca catcaaggga
     4321 ggcgtcattg acagccatgt ccgatgcacc gacggcgaac tggatgaccc tgtcggccct
     4381 cctggcctcc ttgcgatcca tccagcgctc cgggtcgaaa tcttgcacct cggcggctat
     4441 gcgcacggca aaatcggcgg ggtcgaaaag agtgatcccc cctacgccgt tccgcccctc
     4501 cttgagggac ttccagtatt cttcccttcc gttggcaatg ggggagatca cccccatccc
     4561 ggtaatgact acccttctca agtcatgtca ctccttcggt tcccgccgac aggcatcccg
     4621 gcccgggtaa cggagtatgg gggtttatgc tgttggtgtc gcataaaccc ccctaggtcc
     4681 ccttgctact cttccacacc aagcttgccc ttgatgtatt ccatagcttc gccgacattg
     4741 gtgagctttt cggcatcttc atcgggtatt tcgatatcga atacttcctc gatccccatg
     4801 ataagctcga cgatatcaag agaatcggct ccgaaatcct ccacaaagga ggcttccggt
     4861 ttgatctgat cctcttcgac atccagcctg tccattacta tttcctttag tctcgccgtg
     4921 atctcgtcca ttttcatctc cttcacctcc ttcccgattg acccttttca aggatcaggc
     4981 catggtccac ccgccgtcga cggctattac ctggcccgtg atataagaag cctcttcaga
     5041 aacgaggaat cgcaccagag ccgcaacttc ctcgggacgg ccggcccttc cggccgggat
     5101 ctggagcatc atctgggacc gaatcttctc gtcaagaaca gcagtcatgt ccgtttctat
     5161 gaacccaggg gcgatggcat tcaccgtgat ccccctggaa ccaagctctc gtgcggcgct
     5221 tttcgtgagg ccgatcaccc ccgccttcgc ggcggcgtag ttcgcctggc cggggtttcc
     5281 gatcagggcc actacggaac tgatatttac tatacgtccc cacctggaac ggaccatgtc
     5341 cttggcggcc cgccgggtag tcaggaagtt cgatagcagg ttggtcctga tcacatcctc
     5401 ccagtcgggg tccttcatgc gcatcaacag tccatccctg gttatccccg cgttatttat
     5461 gaggatcccc accggcccaa ggtcggattc ggcctcttcg aaaagtctct ccacctcgga
     5521 cgcggaagat acatccgcct ggaaagccct ggcccggcct ccaaaggaca ggatctcctc
     5581 gacaagtctt tccgccgcca cggacgaaga acgatagttt acagcaacgg tatgccccag
     5641 ctctgccatc gcgagggcga tcgccctgcc tatcccgcgc ccggaaccgg tcacaagagc
     5701 aacccgcttt tcaggattca tcatattgtc cccccctcag gaaatcaatc acctcatcga
     5761 ggccatcttc atcaaagagc gaaaaacaat tggcgtcgga ggagatcctt tttatcaacc
     5821 ccgtgagaac gcttcctggc cctatctcaa cgaagtgcct gacccccctg gagatgataa
     5881 attcgacgga gcccgaccag agcacgggca tgaaggtctg caggaaaagc gcttcctgta
     5941 tgcttcccac gtcggtttcc tcttgagcgg acacgttggc taccaacggc cattttggcc
     6001 ttttccactc acattgggca aaggactccc tcaactggtc cgcgacgggc cgcatgaggc
     6061 aggaatggaa gggcgcactg accttgagag gaagagctct cttggcccca gcatctttgc
     6121 acaaggcaag ggctttgtcg accgcgccct tctccccgga tatgacgatc tgacctgggg
     6181 cgttgaaatt ggccgcctgg caaacaccga gggtggaaac ggattcacaa atggatatga
     6241 ccgactcgcg gtcaaggccg atgacagctg ccatagagcc tactcccaag gggacggcct
     6301 cctgcatcag ttttcccctg agatggacca gccttaggcc atcctcgagg gagagagcct
     6361 ccgaggcgac cagggcggtg tattcaccca ggctgtgccc ggccacgtac tcaggggaga
     6421 acacgacacc tttttcctct tccaggcatc tgaaaacggc catgctcatc gcaaggatcg
     6481 ccggttgagc gtattcagtt ttcttaagtt cgtcatcagg gccgaaaaag atgatcctgc
     6541 tcaggttaaa acccaaagca tcgtcggatc tttcaaaaac agccctggag gcggcgtaac
     6601 ggtcaaaaaa gcccttgccc atccccactt cctgtgctcc ctggccgggg aaaacaacgg
     6661 catacttcat tggaatacct ccagaggcat caatggtttg aaaacctgtg gagactctcg
     6721 aacatctcct gcgttccttc gaagagttcc cttatcatct cagctgctgg ttgtatcctg
     6781 ttcacgagcg ccgctgactg accggccatg acggaaccga attccacgtc cccctctacc
     6841 acggccgccc ttagcctgcc tgtgccaagc ttttccagtt cctccacgga ggccccggct
     6901 ttctcaagcc tggcgaattc cctggtaagc ctgttctgga tgcaacggac gggatgcccg
     6961 gtgctggccc cggtgatcgc cgtactcctg tccctggcct tgatgatctc gttcttgaag
     7021 gcctcatgag cgttggattc acaggcacac acgaagcgtg tcccgacctg gaccccctcc
     7081 gcgcccaggg agaaggcggc caggactccc ctgggttcag ccacacctcc ggctgcaacc
     7141 acaggaatat tgacggcatc gactatctgg ggtaccagaa ccatggtcgt gatctcacca
     7201 atgtgccccc ccgcttccat cccctctgct atcacggcat cggccccctg cttttcgact
     7261 ctttttgcgt gagccaccga ggcgatgacc gggatgacct tcgtcccaag gggtttgagt
     7321 cgctcgatca cctttccggg ggctcccgcg cccgtggtca cgacagggac ccggtttctc
     7381 tcgaccactt caagagcggc gtccgcggtg gccgaaagaa gcataatgtt gaccccgaaa
     7441 ggtttattgg tcagggccct gaccttggcg atctggctct cgaggaggtc agggggcatg
     7501 cttcccgcgg caatgacgcc aagtcccccg ccgttgctca ccgcagccgc caggtcagcg
     7561 tcggccaccc aggccatccc gccctggatc accgggtaaa cgaccccaag aagcctggtt
     7621 atcctgttat taatgaacat cgataccacc tcagatccga attatcatgg caccgtaggt
     7681 cattcctgcc ccgaaactca ccagcatgac cttgtcgccc tttgagatct tgccttcagc
     7741 cattccctca tgaagagcca ggaagaccga cgcagccgag gtgttcccat acttctggag
     7801 gttgaccacg gccttatcca gggggatctc aagccttcgc aagacccctt cgattattcg
     7861 atggttggcc tggtgaaaaa cccaagcgtc tatctccctc acatctagac cggcttggct
     7921 gcaaaggtcc ctgacaaagg gtgggatcac cttgttgacg tatttaaaca cttcgttgcc
     7981 tttcattttt acgaagtgga ggccctgttc gaccgtttcc acagtggcgg ggtgggcgac
     8041 aagccccccc ggcaactcga tataatcatc aaggttgccg tcggcatgga cttccgccgc
     8101 cagcacccca tcctcacctt ccccggcacc aaggagggca gccccggcgc catccccgaa
     8161 aagcacacag gtattgcgat ccttccaatc cacgatcctc gagagtactt ccgccccgat
     8221 caccaggact tttttccaga atcctgccct tatacccgcg gaggccagca tcaggccata
     8281 aacacagccc gtgcaccctg cctgaagatc gcaggccccg gctttcgagg caccgatcct
     8341 ggcttggacg atcggaccta cgcccgggaa aagcctgtca ggggagttgg tcccgacaat
     8401 gatcatgtcc agttcttccg gtaagacgga agcggcatcc agggcttcca tcgctgccgc
     8461 cgctgcaagg tcgctggtca attcgcccat ggaggcgatg cgtctttccc tgatccccgt
     8521 cctctccacg atccactcgt cgctggtttc aaccattttt tcaagatctc ggttggtcac
     8581 aaccctcgtg ggaacgctca tacctgtccc aagaagcact acaggggcac atcggttaaa
     8641 attcatcctc tcttcactcc ctcggcaagt tcttcggcta tggtctgtac gcccctttta
     8701 ttcacaaact ctcgagcgac ccttatcgcg ccgcatatgg cccttgccct tgaccgcccg
     8761 tgggctttaa ttaccgcccc gttcacaccg agcaacgggg tgccgccggt ctcctcgtag
     8821 ttgaaacggg cccagaggtc cttaagcatg gggatcatga acaccatgcc catcttagcg
     8881 agtatgcgcc ggtcaatctc ttcgcggatg agtgagtata tcgcttcccc ggtaccctcg
     8941 atgaacttca gcaggatgtt gcccgtaagc ccttcacaga ccacaacatc ggccgctccg
     9001 aaggggatcc cgcctccctc gacgttgccg atgaaattaa gtctcccgtt tttaaaatac
     9061 tcccttgcct cgattatcag ttcatccccc tttgcctctt cctgcccgtt ggaaaggagc
     9121 cctatggagg gactatccac gcctatgatc ttcctcatgt aaacgtcccc catcagggcg
     9181 aactggaaca ggttcagggg tttgcaccgt accgtcgcgc cgatgtctat cagcatcgat
     9241 atccggttca ccgccgggag aggtactccc agccccggcc tttcgatccc gggtatccgc
     9301 cccaccacca ggacgccggc ggccacaatg gcgcccgtgc tccccgccga gacgcaaccc
     9361 tgggcctctc ctgaccggac catctgcatg gcgatcctga ggctcgaacc ccttttcctt
     9421 ctcacggcca cagcgggagg ctcgtccatg gcgatcttct cttcggcatg gaccaggtgc
     9481 agccttttcc ttacaggtgc ggaggtattc atcagaaggg gtgcgatggc ctcagagtca
     9541 cccacgagag ctatctcgag atccgtgaat ttctcacagg ccatgatcgc cccggcgcag
     9601 gtttccccgg gagcgttatc cccacccatg gcctcaagag ccagcaacac tagcctcacc
     9661 ccttccctga gccggggtcc cgtcttgaca ttctttttcc acggttatga accggccgac
     9721 gaagatctcc tgttccccta ctcttattct cacactgacc acattcttgt tacccttttt
     9781 agtacccact tttgctctcg cgacgagcct ttctcccacc ttcgcgggga accggaaccg
     9841 gacccgcgcc gaaccggtga cgaccatgtc ggcctcaata acggcaatgg caagagaact
     9901 ggccatggca tagatgtagt gatcggaagc gtaatcggtg tgtcggaaag ccatctccct
     9961 cgaaacctgg agggtggaga gggcccactc cccgggctgc aggtccagga gttctccaat
    10021 gacctcttcg cgttttaacg acctgagccg gctcccggcc tggttggcca tggacctcat
    10081 ccgttccctg acctcgggga tgccgagcat ggccc
//
LOCUS       DAOSRG010000030         9942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6585_length_9942_cov_8.732882, whole genome shotgun sequence.
ACCESSION   DAOSRG010000030 DAOSRG010000000
VERSION     DAOSRG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_9942_cov_8.732882"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLM30_02205"
     CDS             <1..391
                     /locus_tag="PLM30_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOR54015.1"
                     /translation="GRGHRALDDSLAAMGVFLKCLREIDPAGEMEYGPFERSYSLRSL
                     AALERSAFPLRWPPGFDAIREACENSGSVFITYQSGSGEVTQRIIEPTGLVRVFGMTM
                     LEAWCTLRGESRTFRFDRIIDVKRLQE"
     gene            complement(432..508)
                     /locus_tag="PLM30_02210"
     tRNA            complement(432..508)
                     /locus_tag="PLM30_02210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Arg,seq:acg)
     gene            complement(527..620)
                     /locus_tag="PLM30_02215"
     tRNA            complement(527..620)
                     /locus_tag="PLM30_02215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(584..586),aa:Ser,seq:gct)
     gene            complement(691..1773)
                     /locus_tag="PLM30_02220"
     CDS             complement(691..1773)
                     /locus_tag="PLM30_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOR54016.1"
                     /translation="MQALRTKTRVIMLVVVVVFVVSLFAMYITRGGGPSRHTEGERDF
                     AVAVVDGERVMASQVVAGVRDYVQQTGQSDLSPESIALMRNQVLQNIALGHMLEKEAA
                     RQKIEPSREDIDAAVKRIEKQFPTKEAFQQYMDSSGIKMKALQERIAAQLSQQMVLEA
                     NTPQAEVSDEEARAFYDQAKEVYFRHPAGYNVLYARFGEADKAEEARKALAGGGKWDD
                     VMKGGEGAVQDFTPSAEPAFVAEREFTEGDLKAIADTPLGKVSKALTLGEGNVVIVVK
                     EKKIEEKIVPFEEASADAREMVAYQKKRAGQGEYLQGLLAEAGIQVLAPEFFEAPAPA
                     PTGDEGEGTPEEGASPEGGEEEPSGE"
     gene            complement(1881..>3557)
                     /locus_tag="PLM30_02225"
     CDS             complement(1881..>3557)
                     /locus_tag="PLM30_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOR54017.1"
                     /translation="GSAQAAPAPSSPPAAADLREGTLEELLSGDELALGWAGGGDYPQ
                     DFSIQEFCLCSPDGLFWRGAAEGPALEKLARWTQRGRVTTSGYKELCAAAPSLLPEPG
                     RVWDARLAHYALHPEVKDRSSYGPSPGETMALWEARERLNPQLLSLGLEGVMADIDTP
                     LCQVLASMERRGVRVDKGQLSDLEEELDHRTGEISTQIDNLVGDHVNLNSTKQVAWLL
                     FEKLGYPPVKKIKTGFSTDVSVLEELAALPLSDDGVPRMLLEYREIAKVISGFIQPLL
                     RQTDPVTGSVHTTFEHTVTGTGRLSSRDPNLQNVPTFGNWAGRLRRALKPRGEGNLFL
                     AADYSQVELRILAHICGEERLKEAFAQGRDIHAETAFWIFGGSGEVTPEHRRMAKVVN
                     FGLLYGMGVHGLSQRMGISREEAAAVIGRYFAAFPRVKDYMENSARDAKERGYTKTLF
                     GRIRPLSEVSTVEGRGPGALERVAVNTPLQGTAADISRIAMVRYHRSAARAGLEAPLV
                     LQVHDSLVVECRPGQEEAVTALLRESMEGAADLSVPLAVHIKGGATFEDI"
     assembly_gap    3558..3657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3658..4106)
                     /locus_tag="PLM30_02230"
     CDS             complement(<3658..4106)
                     /locus_tag="PLM30_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR54018.1"
                     /translation="MPERTVLLVDGHGLAFRAFFALPELNAPDGTPTNALLGYANMLL
                     RTLEELKPDLAAVVFDAPGPTFRHEAYEAYKEGRQPTPGEFKAQVPLIKEFSRDLGLS
                     VVERAGVEADDVIASTALSAAGKGYRVVVLTADKDILQVLGENLQVMR"
     gene            4200..5513
                     /gene="gltX"
                     /locus_tag="PLM30_02235"
     CDS             4200..5513
                     /gene="gltX"
                     /locus_tag="PLM30_02235"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR54019.1"
                     /translation="MTSNTRVRFAPSPTGALHIGGAHTALFNWLWARHNGGSFILRIE
                     DTDLARSTEEHERTILQGLSWLGLDWDEGPDVGGSHGPYRQSERREIYLRHAHDLVRS
                     GQAYCEGEAVIFSVPKGRTVAFDDLVYGHIEMESDTLKDVVLIKSDGFPTYNYAVVVD
                     DHLMEITCVVRGEDHISNTPKQLLLYQALGWETPLFAHLPMILGKDKKKLSKRHGATS
                     VYEYRDMGYHPDALFNYFALLGWNPGDDREIFSRDEAVRMFDLTRVTKKAAVFDMDKL
                     NFVNQAHIAMMPPLERARHTFPFWEEAGLDPGSRSEEWLAGVLDLLEGRGRTFRELAE
                     YTDYFLSFEAVKKRWGGEEIDGERREALKGFFTAMLSFPSWKPEELEVMAKAWCDGKG
                     LKMKDVAMPLRWALTGRKVSPGIFHVAAFLGRDEVKKRLSHYGLI"
     gene            complement(5548..5793)
                     /locus_tag="PLM30_02240"
     CDS             complement(5548..5793)
                     /locus_tag="PLM30_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54020.1"
                     /translation="MAAAYPEGARPLVIACSQAEGDVKDPGAYHDVGMAFLSQEGFEV
                     WGQMDYPVEFIHALGCFALMAYPSDPGRVLGKFLRKY"
     gene            complement(5751..6575)
                     /locus_tag="PLM30_02245"
     CDS             complement(5751..6575)
                     /locus_tag="PLM30_02245"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOR54021.1"
                     /translation="MGKVLVIFTGGTIASAFLGERKGPDASASRALHESISSFFAGKN
                     MEVECLEPWGIPGLDSSNLDPGHWLEMTRAIDGAVREGVDGVLILHGTDTMAHTAAWL
                     SLCFSGVGIPVVLTGSQLTLDYTPEDVTVNLRGAAQVVCSDLTGVWIYCNWKLIQGNR
                     AHKARAQHPDAFVPVGGMPLYFAPEWVRGEGIAETPHPRGRTPDPLKKILAMTPDEAR
                     LSGKGIRWLFISPGRPRHRRATRRSWPCWVSARVTPPRHFSSPWRPLTPKGPGPWS"
     gene            complement(6595..7302)
                     /locus_tag="PLM30_02250"
     CDS             complement(6595..7302)
                     /locus_tag="PLM30_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR54022.1"
                     /translation="MINRMMIQLFRQWSGDPATNPFPVAHMPDSLTEALKAAAEGKLE
                     LNDPVQVPEGFRGRIGYDKKTCIGCRLCIKVCPAHAIDYLEEEKKVLFHMDRCCFCAQ
                     CTEICPVHCIWMTDEFAFASYERKKEVVVDSGKRPAAEKPGEPEETKDVKEQKEPKGA
                     ESAAETEGDGGTADAGTATYHIDPDKCIGCTQCSRVCPVQAISGAPREKHVIDEEKCI
                     GCAACAEVCPVKAISPR"
     gene            complement(7315..8307)
                     /locus_tag="PLM30_02255"
     CDS             complement(7315..8307)
                     /locus_tag="PLM30_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOR54023.1"
                     /translation="MTLLQFKVVAGAALLFFALLLSLLFEGVDRVVHARMQLRLGPPV
                     FQPFYDVLKLLGKENIVPRRAIPWAFNGAPWVSAAAAMMILLYVPMGSLPPILAASGD
                     LILILYLLGLSAVAMAVGGFASGSTYANVGAQREMTLMMSYELPLAVVIVTLAWFSYR
                     TGMPGEPFSLETFVGMPVWSVVGWTGLFGLVCLLAALLAVVPAEVGKVPMDIAEAKTE
                     ILEGLICEYSGRNLAMFKLTFSLRTLVMCSVVVSLFFPWTIGLRFGLEGPMLFLADFA
                     FFWVKVFLLQIIGVTTIRTAFGRFKIWQASQFYWFHIAGLSLAGMVLLSLDVIL"
     gene            complement(8304..>8672)
                     /locus_tag="PLM30_02260"
     CDS             complement(8304..>8672)
                     /locus_tag="PLM30_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit"
                     /protein_id="HOR54024.1"
                     /translation="LMWEPKLTKLLSFLKQADGTGLSSIEAPRGDDTHAIRLTGGEDN
                     VTWWKVRAPTYSNAVSWPIMFRNNELADSPLIINSIDPCISCMERMVVTDRSSGHEQV
                     VTRSELVEQCREKTRRLMGR"
     assembly_gap    8673..8772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8773..9182)
                     /locus_tag="PLM30_02265"
     CDS             complement(<8773..9182)
                     /locus_tag="PLM30_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOR54025.1"
                     /translation="MAQGTNKTYKVPIGPVHVGLKEPITAWLDVDGERIKDATIRPGA
                     IHRGIEFMARERNPIQVIYLAERICGICSFSHIVAFTRAVEDATGIEVPARAQYIRAI
                     VLELERIHSHILWAGVACYTFGYDSAFNLGMILR"
     gene            complement(9195..9746)
                     /locus_tag="PLM30_02270"
     CDS             complement(9195..9746)
                     /locus_tag="PLM30_02270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOR54026.1"
                     /translation="MSPHRRSYTDEYLDPEKVLSLLKKKFRKTSDDMVLREYRGGAAN
                     GGSYQCLWVRVEKQRFLDMVDTLMEIDFPHFQITSGDDIGDAVELNYHFSIFRSVGRG
                     KRLGVTVSVVVPKDDLVMPSLWERIPGTEYSEREIREMFGVDFDGLPNKGLVFLPEDW
                     DEDIKPWRRDETGPKPEDIRELS"
     gene            complement(9743..>9942)
                     /locus_tag="PLM30_02275"
     CDS             complement(9743..>9942)
                     /locus_tag="PLM30_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOR54027.1"
                     /translation="GNCAGSGDVFYKSYNLDGPVDNIIPVDVYVHGCPPRPEAIIEGV
                     AKAVLKLEKLKESLAKAGAAK"
BASE COUNT         1667 a         3098 c         3140 g         1837 t
ORIGIN      
        1 ggggcgcggc cacagggccc ttgacgattc gctggcggcg atgggggtct tcctgaagtg
       61 cctccgggag atagacccgg cgggtgagat ggaatacggc cccttcgaga ggagttactc
      121 cctccgatcc ctggcggccc tcgagaggag cgcgttcccc ctgcgctggc cccccggttt
      181 cgacgccatc agggaagcct gcgagaactc cgggtcggtg ttcatcacct accagagcgg
      241 aagcggcgag gtgacgcaga ggatcatcga gcccacgggc ctggtaaggg tcttcggcat
      301 gaccatgctc gaagcctggt gcacgctccg gggggagagc cggaccttcc gcttcgacag
      361 gatcatcgat gtaaaaaggt tgcaggaata aaaaagacgc ccccccaaag ggggcgtctt
      421 tcgtcatttt atggcgcgcc ggacaggagt cgaacccgta accttcggat ccgtagtccg
      481 atgctctatc caattgagct accggcgcgc gatgcctggg gccctatggc ggtgggacag
      541 ggattcgaac cctggagaga ggtttaagcc cccctactcg cttagcaggc gagtgccttc
      601 agccgctcgg ccatcccacc ctgtgcgttt acgcgcggtc tattctaggc acccccgggg
      661 ggagtgtcaa gaaaaaagtc ccctcctccg tcactccccg gagggttcct cctctccgcc
      721 ctcgggggac gcgccctctt cgggggtccc ttccccctcg tcacccgtcg gagccggggc
      781 cggcgcttcg aagaactccg gggccagcac ctggatgccg gcctcggcga gcagcccctg
      841 gaggtactcg ccctggccgg cccgcttctt ctggtaggcg accatctccc tggcgtcggc
      901 gctggcctct tcgaagggga cgatcttctc ctcaattttc ttttccttga cgacgatgac
      961 cacgttgccc tcgcccaggg tcagcgcctt tgagaccttg ccgaggggcg tgtcggctat
     1021 agccttgagg tcaccctcgg tgaactccct ttcggcgacg aaggcgggct ctgccgaggg
     1081 ggtgaaatcc tggacggctc cctcgccgcc cttcatgaca tcgtcccact tgccgccgcc
     1141 ggctagcgcc tttctggcct cttcggcctt atcggcctct ccgaagcggg cgtaaaggac
     1201 gttgtacccg gcggggtggc ggaagtagac ctccttggcc tggtcgtaga aggcgcgggc
     1261 ctcctcgtcg gagacttccg cctggggggt gttggcctcg aggaccatct gctgggacag
     1321 ctgggcggcg atgcgctcct ggagggcctt catcttgatg ccgctgctgt ccatgtactg
     1381 ctggaaggct tccttcgtcg ggaactgctt ctctatccgc ttgacggcag cgtcgatatc
     1441 ctcccgggag ggctcgatct tctgcctcgc cgcttccttc tcgagcatgt gtcccagggc
     1501 gatgttctgc aggacctggt tgcgcatgag ggcgatggac tcgggcgaaa ggtccgactg
     1561 ccccgtctgc tggacgtagt ccctcacacc ggcgaccacc tgggaagcca tgaccctttc
     1621 gccgtccaca acggccacgg cgaagtccct ctcgccttcg gtgtggcggg agggcccgcc
     1681 gcccctggtt atgtacatgg cgaaaaggga tactacgaag accacgacca ccacgagcat
     1741 gatcacgcgg gtttttgtcc tgagagcctg cattatcacg ttatatcttc ctccttaggc
     1801 cttggggcgg gacatttttt gtgaatttcg cagcgtgtcc aatgatacgt tcccttaccg
     1861 gtgctgtcaa aggcttttcc tcatatatcc tcgaaggtgg cgccgccctt tatgtggacg
     1921 gccaatggga ccgacaggtc tgccgcccct tccatggact cccggagcag cgccgtcacg
     1981 gcctcctcct ggccggggcg gcactccacc accagcgagt cgtgcacctg gagcaccagc
     2041 ggggcttcca ggcccgcccg ggccgcggag cgatggtagc gcaccatggc tattctggat
     2101 atgtcggcgg cggtgccctg gaggggcgtg ttgacggcca ccctttccag ggcgcccggt
     2161 cccctcccct cgacggtgga gacctccgag agggggcgta tcctcccgaa gagggtcttc
     2221 gtgtatcccc gctccttggc gtcgcgggcc gagttctcca tgtaatcctt gaccctgggg
     2281 aaggccgcga agtaccggcc gatgacggcc gcagcttcct cgcggcttat gcccatcctc
     2341 tgggaaagac cgtggacgcc catgccgtag aggaggccga agttgaccac cttggccatc
     2401 cgccggtgct cgggggtgac ctcgccgctg ccgccgaaga tccagaaggc cgtctcggca
     2461 tggatgtcgc gcccctgggc gaaggcctcc ttgaggcgct cctcgccgca aatatgggct
     2521 aggatcctca gttccacctg ggagtagtcc gccgccagga acaggttgcc ctcgccgcgg
     2581 ggtttcaggg cccggcggag cctgcccgcc cagttgccga aggtcggcac gttctggagg
     2641 ttggggtccc ggctgctgag gcggcccgtc ccggtgacgg tatgctcgaa ggtggtgtgg
     2701 acgctcccgg tgacggggtc ggtctggcgc agaaggggct ggatgaagcc ggagatgacc
     2761 ttggcgatct cccggtactc cagcagcatc ctggggaccc cgtcgtcgct taaggggagg
     2821 gccgccagtt cctccaggac cgagacgtcg gtggaaaagc ccgtttttat cttcttcacc
     2881 ggcggatagc ccaatttttc aaagagaagc caggcgacct gcttcgtgga gttgaggttc
     2941 acgtggtcgc cgaccaggtt gtctatctgg gtggatatct cgccggtgcg gtggtcgagt
     3001 tcctcctcga ggtccgaaag ctgccccttg tccacccgga cgccccgcct ctccatggag
     3061 gccaggacct ggcagagggg ggtgtcgatg tccgccatca ccccttccag gcccagggag
     3121 aggagctgtg ggtttagcct ttcccgggcc tcccaaaggg ccatggtctc ccccggcgag
     3181 gggccgtagg acgaccggtc cttgacctcg gggtgcaggg cgtagtgcgc caggcgcgcg
     3241 tcccagaccc tccccggttc gggcagcagc gacggtgccg cggcgcaaag ttccttgtag
     3301 ccgcttgtgg tgacccggcc ccgctgggtc caccgcgcca gtttttccag ggccgggcct
     3361 tcggcggcgc ccctccagaa gaggccgtcc ggggagcaaa ggcaaaactc ctggatggaa
     3421 aagtcctggg ggtaatcgcc ccccccggcc cacccgaggg ccagttcatc cccggagagc
     3481 agttcctcca gggttccctc gcgaagatcg gccgcggccg gtggggacga aggggccggc
     3541 gccgcctggg ctgacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
     3661 atcacctgaa ggttttcgcc caggacctgg agtatgtcct tgtcggccgt gaggacgacc
     3721 acgcggtagc cctttccggc ggccgagagg gccgtcgagg ctatgacgtc gtcggcctcg
     3781 acgcctgccc tttccaccac ggaaagaccc aggtccctgg aaaattcctt gatgaggggc
     3841 acctgggcct tgaattcccc cggtgtcggc tgccgaccct ccttgtaggc ctcgtaggcc
     3901 tcgtgccgga aggtgggccc cggagcgtcg aagacgacgg cggcgaggtc gggcttcagt
     3961 tcctccaggg tcctgaggag catgttggcg taccccagga gggcgtttgt aggggtgccg
     4021 tcgggcgcgt tcaattcggg cagggcgaag aaagccctga aggcgagccc gtggccgtcc
     4081 acgagaagta ccgtcctttc cggcatttgc ggcatctcct tcgtccgggt tttggggtat
     4141 actgctgttc gaccccaatg ctaccggata ggataccatc agggaaggat gaacaggtaa
     4201 tgacttcgaa caccagggta cgcttcgctc ccagtcccac gggggccctc cacatcgggg
     4261 gggctcacac ggccctcttc aactggctct gggcccgtca caacggcggc tccttcatcc
     4321 tgcgcatcga ggacacggac ctggcccgtt ccaccgagga gcacgagagg acgatactcc
     4381 aggggctcag ttggctgggc ctcgactggg acgagggccc cgacgtgggg ggaagccacg
     4441 gcccctatag gcagtctgag agaagggaga tctacctccg ccacgcccac gacctggtaa
     4501 ggtcggggca ggcctactgc gaaggcgagg ctgtcatctt ctccgtcccc aagggcagga
     4561 cggtggcctt cgacgacctc gtctatgggc acatcgagat ggaaagcgac accctgaagg
     4621 acgtcgtcct tataaagagc gacggtttcc caacctacaa ctacgccgtc gtcgtcgacg
     4681 accacctgat ggagataacc tgcgtggtca ggggcgagga ccatatctcg aacacgccca
     4741 agcagctcct cctctaccag gccctgggat gggagacgcc ccttttcgcc cacctgccga
     4801 tgatcctcgg caaggacaag aagaaactat ccaagaggca cggcgccacc agcgtctatg
     4861 agtaccgcga catgggctac caccccgacg cgctcttcaa ctacttcgcg ctgctggggt
     4921 ggaaccccgg ggacgaccgg gagatcttct cccgggatga ggccgtgagg atgttcgacc
     4981 tgacgagggt tacgaagaag gcggccgtct tcgacatgga caagctgaac ttcgttaacc
     5041 aggctcacat agccatgatg ccccccctgg agagggctcg gcacaccttc cccttctggg
     5101 aggaggcggg cctggacccc gggtcgcgga gcgaggagtg gctggcgggg gtcctggacc
     5161 tcctggaagg gcggggccgg actttcaggg aactggcgga gtacaccgac tacttcctct
     5221 ccttcgaggc ggtgaaaaag cgctggggcg gggaggaaat cgacggggaa cgcagggagg
     5281 ccctcaaggg tttcttcacg gccatgcttt ccttcccctc ctggaaaccg gaggaactgg
     5341 aagtgatggc caaggcctgg tgcgatggga aggggctgaa gatgaaggac gtggccatgc
     5401 cgctccggtg ggccctcacg ggccgcaagg tgagcccggg aatattccac gtggccgcct
     5461 tcctgggcag ggacgaggtc aaaaaaaggc tttcccacta tggcctgatc tagcggacct
     5521 ctcgtcgggc cgttttttcg tcttttttta gtacttcctg aggaacttgc ccaggaccct
     5581 cccagggtcc gaagggtaag ccatgagggc gaagcagccc agggcatgga tgaactccac
     5641 ggggtagtcc atctggcccc agacctcgaa accctcctgg gagagaaagg ccatgcccac
     5701 gtcgtgatag gcgcccgggt cctttacgtc gccctcggcc tggctgcagg ctatgaccag
     5761 gggcctggcc ccttcggggt aagcggccgc cacggactcg agaagtgacg tgggggcgtt
     5821 acccgcgccg aaacccagca gggccaggat cttctcgtcg cccgccggtg gcgtggccga
     5881 cccggtgaga tgaagagcca ccggatgccc tttcccgaaa gccgcgcctc gtcgggggtc
     5941 atcgccagga tctttttgag gggatcgggg gtcctccccc tggggtgggg cgtttccgct
     6001 atcccttcgc ccctcaccca ctccggcgca aagtaaaggg gcattccacc cacggggacg
     6061 aaggcatcgg ggtgctgcgc cctcgccttg tgggccctgt tgccctggat gagtttccag
     6121 ttgcagtaga tccagacccc cgtcaggtcg gaacagacca cctgggcggc accccgcagg
     6181 ttcaccgtga catcctcggg ggtgtagtcc agggtgagct ggctgccagt gaggaccacc
     6241 ggtatgccta cgccggagaa gcagaggctg agccaggccg cagtgtgggc catggtgtcg
     6301 gtgccgtgga ggatgaggac cccgtcgacg ccctccctta cggccccgtc gatggcccgg
     6361 gtcatctcca gccagtggcc ggggtcgagg ttcgaactgt cgagccccgg tatcccccag
     6421 ggttcgaggc attccacttc catgttcttc ccggcgaaga aggaggagat gctctcgtgc
     6481 agggccctcg aggccgaggc gtccggcccc tttctctcgc ccaggaaggc gctggctatg
     6541 gtgcccccgg tgaagatcac cagtaccttt cccatggggc gccgcccttc ccccctacct
     6601 ggggctgatg gccttgacgg ggcagacctc cgcgcaggcc gcgcagccta tgcacttctc
     6661 ctcgtcgatg acgtgcttct ccctgggcgc gcccgaaatg gcctggacag gacagaccct
     6721 ggagcactgg gtgcagccga tgcacttgtc agggtcgata tggtacgtgg ccgtccccgc
     6781 atcggcggtc ccgccgtcgc cttcggtttc cgcggcgctc tcagcacctt ttggctcttt
     6841 ttgctcttta acgtcttttg tctcttcagg ctccccgggt ttttcggcgg cgggcctttt
     6901 gcccgagtcc accacgacct ccttcttgcg ctcgtaggag gcgaaggcga actcgtcggt
     6961 catccagatg caatgcacgg ggcatatttc ggtgcattgg gcgcagaagc agcagcggtc
     7021 catgtggaag aggaccttct tctcctcctc aaggtaatcg atagcgtggg ccgggcagac
     7081 cttaatgcag agcctgcatc cgatgcaggt cttcttgtcg taccctatgc gcccccggaa
     7141 cccctcaggt acctggacgg ggtcgttaag ttccagtttg ccctcggcgg cagccttgag
     7201 ggcctcggtg aggctgtcgg gcatgtgggc cacggggaag gggttggtag ccggatcccc
     7261 gctccactgc ctgaataact ggatcatcat cctgttgatc atttcaaccc ccccttaaag
     7321 gatcacgtcg agggacagca gcaccatacc ggccagggac aggccggcga tgtggaacca
     7381 gtagaattgc gaggcctgcc agatcttgaa ccgcccgaag gccgtcctta tggtcgttac
     7441 gccgatgatc tgaagcaaaa agaccttgac ccagaagaag gcgaagtccg ccaggaagag
     7501 catcggcccc tcgagcccga accggaggcc gatggtccag ggaaaaaaga gggagaccac
     7561 cacggagcac atgaccagcg tcctcaggga gaaggtcagc ttgaacatgg ccaggttgcg
     7621 gccggaatac tcgcagatga gcccctcgag gatctccgtc ttggcttcgg ctatgtccat
     7681 gggcaccttc cccacctcgg cggggaccac ggccaggagg gccgccagga ggcagaccag
     7741 gccgaagaga cccgtccagc ccacgacgct ccagacaggc atccccacga aggtctcgag
     7801 gctgaagggt tcgccgggca tgcccgtccg gtaggagaac caggccagcg tgacaatgac
     7861 caccgccagg gggagctcgt agctcatcat gagggtcatc tcccgctggg cgcctacgtt
     7921 ggcgtaggtg gaaccgctgg cgaaaccgcc cacggccatg gccaccgccg acaggcccag
     7981 caggtagagg atcaggatca ggtcgccgct cgccgccagt atcggtggga gcgaacccat
     8041 ggggacgtaa agaaggatca tcatcgccgc ggcggccgag acccagggtg ccccgttgaa
     8101 agcccagggt atggccctgc ggggcactat gttctccttg cccagcaact tcaggacgtc
     8161 gtagaagggc tggaagaccg gcgggccgag ccgcagctgc atccgggcgt ggaccaccct
     8221 gtcgaccccc tcgaagagca gggagaggag cagcgcgaaa aacagcaggg ctgctcccgc
     8281 gaccaccttg aattgaagaa gggtcatcgt cccatcaacc tcctcgtctt ttcgcggcac
     8341 tgctcgacca gttcggacct ggtcacgacc tgctcatggc cggaggaccg gtcggtgacc
     8401 accatgcgct ccatgcagga aatgcatggg tcgatgctgt tgatgatgag cggcgagtcg
     8461 gccagttcgt tattccggaa catgatgggc cacgagacgg cgttggagta ggtcggggcg
     8521 cggaccttcc accaggtgac gttatcctcg ccgccggtga gccttatggc gtgggtgtcg
     8581 tccccccggg gggcctcgat ggaactgagt ccggtcccgt cggcctgttt taagaaacta
     8641 agcagtttgg tcagtttggg ttcccacatg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nntccctcag gatcatcccc aggttgaagg ccgagtcgta gccgaaggtg
     8821 tagcaggcca ccccggccca cagtatgtgg gagtggatcc tctcaagttc cagtacgatg
     8881 gcccttatgt actgggccct ggccggcacc tcgatgccgg tggcgtcctc tacggcccgg
     8941 gtgaaggcca cgatatggga gaaggagcat atcccgcata tacgttcggc cagatagatt
     9001 acctggatgg gattgcgctc gcgggccatg aactcgatgc cccggtggat ggcgccgggc
     9061 cggatggtcg cgtccttgat acgttccccg tcgacatcga gccaggccgt gatgggttcc
     9121 ttgaggccga cgtgaacggg cccgatgggc actttatagg ttttgttcgt tccttgcgcc
     9181 ataggaggca cctcctagga gagttccctg atgtcttccg gcttcgggcc ggtctcgtcc
     9241 ctgcgccagg gcttgatgtc ctcgtcccag tcctcgggga ggaaaaccag gcccttgttg
     9301 ggcagcccgt cgaagtccac gccgaacatc tcacggatct ccctttcgct gtattccgtc
     9361 ccggggatcc tctcccagag ggagggcatc actaggtcgt ccttgggcac gacgacggag
     9421 acggtgacgc caagcctctt gccccggccg acgctgcgga agatcgagaa gtgatagttc
     9481 agctcgaccg cgtcgccgat gtcgtcgccg gaggttatct ggaagtgggg gaagtcgatc
     9541 tccatcaggg tgtccaccat gtccaggaag cgctgcttct ccacccgcac ccacaggcac
     9601 tggtaggaac cgccgttggc cgcacccccc ctgtattccc gcaacaccat gtcgtcggag
     9661 gtcttgcgga acttcttctt cagcagcgag agaaccttct cggggtccag gtattcatcg
     9721 gtgtaactcc ttcggtgggg gctcatttcg cggcacctgc cttcgcgagg gactccttaa
     9781 gcttttccag cttcagcacc gccttggcga cgccctcgat gatggcctcc ggccttgggg
     9841 gacacccgtg gacgtagacg tcgacgggta tgatgttgtc caccgggccg tccaggttgt
     9901 aggacttgta gaagacgtcg cccgagcccg cgcagttccc ca
//
LOCUS       DAOSRG010000031         9812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6676_length_9812_cov_8.203034, whole genome shotgun sequence.
ACCESSION   DAOSRG010000031 DAOSRG010000000
VERSION     DAOSRG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_9812_cov_8.203034"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLM30_02280"
     CDS             complement(<1..1260)
                     /locus_tag="PLM30_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOR54028.1"
                     /translation="MSQGKLKRDLSFITMVSIAAGAVIGGWLAEAPYWFSVSGAGSAF
                     IFPILGILLVPVGLAFAELTAMLPFASAVDVWTTNAYGPTIGWSTQWMMFLVQVVEPP
                     LMAFIFLTALNHFIPIPAGMTKAVAIGMVFCWYVLSNFEIKLTGLLANIFFFSMVAMS
                     LYVATRFFTSGSWSVSNITSHGGFFPKGSYGIFIAMAVFTLKYIGFEFTPTLIEETNF
                     PASKMWLVLLSALFIPAVLYCIVVLAMGGMAPWEEIAGMAMPEPEIVAKLGLPAIIGI
                     VAIIAGILHALTTLMGFWVSSARCLYGAAQLNQLPKIFMEVNRYGQPWVSNLTVLFFS
                     VFFCLFTGANWVQYIYAVSCIAAGVVYFMCCLDAMKLRKTHPEWERPYRAPGGDMIFV
                     IGLFISVWIAIGSALELEIGGYISLGIY"
     gene            complement(1298..1987)
                     /locus_tag="PLM30_02285"
     CDS             complement(1298..1987)
                     /locus_tag="PLM30_02285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOR54029.1"
                     /translation="MRVNDRYVSLYYEDDHEIGKVVIEREKTALLIVDLQRVFLTRPT
                     EANLPEERKASAKKWKPFYDKIDKVVLPNNRKILKAFREKKMEVIFAKIQGYKKDGRD
                     RSLVHKATGFNELLLPPGSKDAEIVPELAPIEDEIVITKTTDSAINGTKLALILKNMG
                     IDTVVVTGVFTDQCVSGTVRGLADESFKVWLIEDGCMAATEEIQSHELAVLNNIYCHV
                     INTKELLEAII"
     gene            complement(2293..3777)
                     /locus_tag="PLM30_02290"
     CDS             complement(2293..3777)
                     /locus_tag="PLM30_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOR54030.1"
                     /translation="MKKRVLVIGSGGAGLVAAIAARKTGADVQVVSKASRGLGTCTAH
                     AGGIFTLACGGLSPEEHYRKTLETGHHQNDEKLVKVLSEEAEKTLRELARWGVTIEFE
                     SGGASVLSTAPNMVMGGAGFTAELIAIAEGSGVEFVDWTSVMEITVRSGRVVGARVQD
                     WRTGEEGFLRAGSVILATGGGGQIFSNTDNPGRITGDGYALALGAGAVLRDMEFVQFY
                     PLGWAEPGFPRWIVDLSLVDFVRVTDCRGVEFLKRTLDEWGYRNGAEGGLFARDRSAR
                     LVAERDREGGVYAHLEDLEENSFDDPAVRYCLGLDASRFKDFARPLRVAPLEHYFCGG
                     VVIDSRGRTAVDGLFACGEVTGGVDGASRVGGNALSSVAVFGLRTGEAAAEEAGGYNG
                     TGSGPVPGFLHEVSQSVGQSPLALRKELQRETWKALGLIRRTSEMESYLGFLSDFKAR
                     KIRIDTPFDRLLALEMRGLFETAKAVAEAALKRKESLGNHWRSD"
     gene            complement(3909..5189)
                     /locus_tag="PLM30_02295"
     CDS             complement(3909..5189)
                     /locus_tag="PLM30_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOR54031.1"
                     /translation="MEAIILFSILIVTIIVSIPIGITLGLSTCIAMMLTSDIPMLMIA
                     QKAVTGLDSFPLLAIPFFILAGALMCTGGISRRLVALAESLVGFIVGGLAMVTVLACM
                     FFAAISGSGPATVSAIGSFMIPSMKERKYDAGFAAAITAAAGTIGVIIPPSIPFVIYG
                     VVAQCSIGDMFIAGIIPGIIIGLALMSVCYFTARKRNYISMTERPKFSEVSKAFKEAV
                     WALLVPVIILGGIYGGVFTPTEAAVVAVVYSVIIGKFVYRELDMKALYDCLKSTGLIN
                     GATSFLIGLSMAFASYLAMAQIPAKIAALLVNFVDSPFLLMMIINVFLLVLGCFVDNI
                     PAVIILTPILLPVVRMIGIDTIHFGLIITVNLAIGFISPPYGINLFVASAISGESIES
                     ISKEIIPSFLAMVACLLLFTYVPALSMGLVRLLR"
     gene            complement(5198..>5419)
                     /locus_tag="PLM30_02300"
     CDS             complement(5198..>5419)
                     /locus_tag="PLM30_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOR54032.1"
                     /translation="SFCFFLAWQGTKLLIFLQETGQISTAMSMPMTWPYASVPVASAL
                     MCLRLIVEMYDLHKRGLPKPADPVVDIPE"
     assembly_gap    5420..5519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5520..5859)
                     /locus_tag="PLM30_02305"
     CDS             complement(<5520..5859)
                     /locus_tag="PLM30_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOR54033.1"
                     /translation="MTVRKFLHNFEEYFCVWIMAVMTVLVFFQVVMRYVFSNSLSWSE
                     ELARFIFLWLSWMGASYAVRERSHFRVEMFVNMIKGKTRIFFEYIILLAWFSFCFFLA
                     WQGTKLLIFLQ"
     gene            complement(5932..6924)
                     /locus_tag="PLM30_02310"
     CDS             complement(5932..6924)
                     /locus_tag="PLM30_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOR54034.1"
                     /translation="MRKLLAVFTVMAVVAVMAGTAFAAPEYTIKVAYIGSDSHPTMRA
                     MKEYFVKPIEEGSQGRIKVELYPNAQLGGDREISEGVQMGTIQMAIPASAPLAGFDKR
                     VQVLELPYLFTTKQAAFEAADGILGEKLNSYLAAKGMLILGFQENGFRHVTNNRGPVK
                     TPADLKGIKIRTMENPIHLAFFKAVGANPTPMSWGELYTALQQGTVDAQENPYAMIVD
                     GKFYEVQKYVSETGHVFSFEVLIANKKFMDNLPADLNALVVKSAREATLKQREYMDEE
                     EAAFKDEVLKAGMIANSLTPEEKQPFVDAAEKVYPMFEKDFGEEVMEILKQVRK"
     gene            complement(6995..7420)
                     /locus_tag="PLM30_02315"
     CDS             complement(6995..7420)
                     /locus_tag="PLM30_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOR54035.1"
                     /translation="MSQDVKTYKCRKIVKGCAGGEAVVSRDAMCFYLTDPETGEVIER
                     NHAIHGKSIANKILVLKSGKGSSVVQVDGFYQLWMKHNLPAAIILVETEPVIVSAAVM
                     VECTMVDRMEADPYEVIEDGDHVEVDAEKGEVRVRKEAA"
     gene            complement(7420..8637)
                     /locus_tag="PLM30_02320"
     CDS             complement(7420..8637)
                     /locus_tag="PLM30_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOR54036.1"
                     /translation="MFLTDEEKRILDGSEGPGKQKAMEMLYALGLTFDAEKLIPVKRA
                     HVALSGQEGDTYWCELLVSGGATCAITPTTNPYWDTCYLTKFYEVTKEELDLANRTGD
                     AYRKIGAKLTYHCAPGLSTGVPFFGEHIAYSESSATPYVNGALGARSNRESSISALAA
                     AVIGKTPYYGFHIDENRHGHLLIEVEADVKDCYDWGILGHVVGELAGYHNPVLRFKHK
                     VNPGPEDYLYFGAEAATSGAVAMYHMVGVTPEAPTIEAAFGGGKVPAAAAVITDKDLK
                     DREAKLTPATGDINLVMLGCPHYTYDQILEIDRLFAGRKVSHNTAFWILAEAGAVELA
                     ERSHLRQKLELLGVRMVGDTCIDEPCWKSFEGHLGLTDSPKCAYYRERRGQPFAIRRL
                     SECVEAAIKGRID"
     gene            complement(8654..>9812)
                     /gene="larA"
                     /locus_tag="PLM30_02325"
     CDS             complement(8654..>9812)
                     /gene="larA"
                     /locus_tag="PLM30_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOR54037.1"
                     /translation="SPLGTPPLKELARGKKNIVFLVEDSTRSTPLDIIMPVITGYLNE
                     NGVPDGAMSFLTAPGTHRLMTDGEILEKLGPEMVRRFKVVQHDATAREDMVDIGTVMA
                     GNYEIPAHVNRYALASDLLVGIGNIVPHCDAGYAGGAKILQPGVCDFVTTSATHAAAG
                     FCPDIPLGVADGNPCREGIEEVARIAGLAFIVNIVKNYRNEIAGVFSGDFIKAHREGI
                     KLAEKSFRITVPEPADIVIVSSWPSDMDYWQATKGVSCAYFAVKKGGVIIFAAPCGEG
                     LAHNHPLFREWLSLPLDEALRRLRAASPEDVEADMVSAVLAICNCRARNKARIFSVTE
                     GLSKEDIAAMQYTPFPTVQEALDRALELIPGATIGIIPMGGISLPIIGRSQ"
BASE COUNT         2136 a         2828 c         2628 g         2120 t
ORIGIN      
        1 gtagatcccc agcgaaatat agccgccgat ctccagttca agcgctgacc cgatcgctat
       61 ccacaccgaa atgaacaacc cgatgacgaa gatcatatcc ccacccggcg ccctgtaggg
      121 gcgttcccac tcggggtggg tcttgcggag tttcattgcg tcaaggcaac acatgaaata
      181 gaccaccccg gcagctatac aggatacggc ataaatatac tggacccagt tggccccggt
      241 gaacaggcag aaaaacaccg agaagaaaag taccgtcagg ttggacaccc atggctgtcc
      301 ataacggttc acctccatga agatcttggg taactggttg agttgggcgg ccccatacag
      361 gcaacgggca gaggaaaccc agaaccccat aagggtggtc agcgcgtgga ggatgccggc
      421 gatgatcgcc actataccga ttatcgccgg tagacccagc ttcgcgacga tctcgggttc
      481 gggcatcgcc atgccggcta tctcctccca aggcgccatg cctcccattg caaggacgac
      541 gatgcagtaa agaacggccg ggatgaaaag agccgatagg agaacgagcc acattttact
      601 tgccggaaag ttcgtctctt caatcaaggt aggcgtaaac tcgaacccga tatacttcag
      661 ggtaaaaacc gccatggcga tgaagatccc gtaagacccc ttcgggaaaa accccccatg
      721 actcgtaata tttgaaaccg accaactgcc acttgtaaaa aatcttgtcg caacgtacaa
      781 cgacatggcg accattgaaa agaagaagat attggctagt aaacccgtca gtttgatctc
      841 gaagttggac aggacgtacc agcaaaatac catgccgatg gcaacagcct ttgtcattcc
      901 cgcgggaata gggatgaaat ggttcagcgc cgtcaggaaa atgaacgcca tcagcggcgg
      961 ctccaccacc tgtacaagga acatcatcca ttgggtactc cacccgatcg tcggcccgta
     1021 tgcgttggtg gtccaaacgt caacggccga ggcgaagggg agcatcgcgg tcagctctgc
     1081 gaaggcaagc ccaaccggta ccaggaggat gccaagaatg gggaagatga aagcggaccc
     1141 cgctccgctg acgctgaacc aatagggtgc ctccgcaagc cacccgccaa taacggctcc
     1201 cgccgcgatc gaaaccatgg tgataaacga aagatcccgc ttcagcttac cctgggacac
     1261 ctgccttacc tcccttcagg gaacataagt attaaattca gattattgcc tctaataact
     1321 ccttcgtatt gatgacgtgg caataaatat tgttcaaaac agccagttca tggctctgga
     1381 tctcctccgt ggccgccatg caaccgtctt cgataagcca gaccttgaaa ctctcgtcgg
     1441 ccaggcctcg taccgtcccg gaaacgcatt ggtccgtaaa tacaccggtg accaccaccg
     1501 tgtcgatacc catgttcttg aggatcaggg ctaacttggt cccattgata gcgctgtcgg
     1561 tggtcttggt gatcactatc tcatcctcga taggggccag ttccggcacg atctcggcat
     1621 cctttgagcc gggaggcagc aacagctcgt tgaagcctgt cgctttgtgg acaagggaac
     1681 gatccctgcc gtccttcttg tagccctgga tcttcgcgaa gatcacctcc atctttttct
     1741 cccggaaagc cttgagtatc ttccgattgt tcggaaggac caccttgtcg atcttgtcgt
     1801 agaaaggctt ccacttttta gcggaagctt ttctctcttc cggcaggttc gcttccgtgg
     1861 gccttgtcag gaataccctt tgaaggtcca cgatcagaag ggccgtcttt tccctttcga
     1921 tcactacttt gccgatctca tgatcatcct cgtagtaaag ggatacgtac cggtcattta
     1981 ccctcataac ccatctcctc cttggaagca gactccgggt tttttcggga ttggcggcaa
     2041 aggggaaagt attgcgggtg gtttaagata ttgggctcag caaagcgaaa ataacgaaat
     2101 agaggaaatg tgaaagggtg tttcggcata caaaatgttc gtcaaacccg taacgaaatg
     2161 atactccctt cttcacggca gtcaagaggg ataaactgat ggctggatga agggatcatt
     2221 ggctaaatag cgtgtcagat aggagggagg ccctgtcttt atcctgccaa gcctccgaaa
     2281 acggtcttac tatcaatccg atctccagtg gttacccagg ctttcttttc tttttagtgc
     2341 cgcttcggcg acggccttcg ccgtctcgaa aagtccgcgc atttcaagag ccagcaaccg
     2401 gtcgaaaggg gtatcgatcc gtatctttct tgccttgaag tccgaaagaa agcccaggta
     2461 gctctccatt tcactggttc tcctgatgag acccagggct ttccaggttt cccgctgcag
     2521 ttctttccgc agcgccaggg gactttgtcc cacgctttgg gatacttcgt gaagaaaacc
     2581 tggaacaggt ccggatcctg ttccattgta tcccccggcc tcttcggcgg cggcctcccc
     2641 ggtacggaga ccaaaaacgg caacgctgct gagggcgtta ccccctactc ggctggcccc
     2701 atcgacgccg cccgtcacct caccacaggc gaacaaaccg tcgacagcgg tacgccctcg
     2761 gctgtcaatg acgactccgc cgcagaagta gtgctccagc ggagcgaccc taagagggcg
     2821 ggcaaaatcc ttgaagcgcg aggcgtccag tcccaggcag tatctaacag ccgggtcgtc
     2881 gaagctgttt tcttccaggt cctccagatg agcataaacg ccaccttccc tgtccctttc
     2941 ggccaccagc ctagcgctcc tgtcacgggc gaaaaggccg ccttcagccc cgttccggta
     3001 gccccattcg tccagggttc ttttcaggaa ctccactccc cggcagtccg taaccctgac
     3061 aaagtcaaca agggaaaggt cgactatcca ccgcgggaag cctggttccg cccaacccag
     3121 aggatagaac tgcacgaact ccatgtcccg caggaccgcc cctgctccca acgccagggc
     3181 gtacccgtcg ccggttatcc tgccggggtt atcggtattg ctgaatatct gtccacctcc
     3241 cccggtggcc aatatcacgg agcccgccct cagaaagccc tcttctccgg tccgccaatc
     3301 ctggaccctg gctccgacca cccgtccact ccttacggta atctccatga ctgaagtcca
     3361 atccacaaac tccactccgg atccttcggc gatagcgatg agctccgcgg tgaaaccggc
     3421 gccgcccatg accatgttag gggccgtgga caagaccgag gcccctccac tttcaaactc
     3481 gatggtgacc ccccatcgag cgagttccct gagagttttc tctgcctctt cggagaggac
     3541 ctttactagt ttttcgtcat tttgatgatg gcccgtctcg agggtcttcc ggtaatgttc
     3601 ttcgggggaa aggcccccgc aggccaaggt gaatatgccc ccggcatggg cagtgcatgt
     3661 gccaaggccc cgacttgcct ttgagacgac ctgaacatcc gctcccgtct tcctcgccgc
     3721 gatggccgcg accaggccgg cgccgccgga tccaatgacc aggacacgtt ttttcacgct
     3781 actcctcctc accggacagg ataacaaagg ggtccggatg ccggtatttt acccctgcaa
     3841 gtccctaaaa aaggaaaggc ggccggcccg atcccaccgg tcgccccttg gacccaaagt
     3901 gtgagactct atcttaacaa ccggaccagc cccatggaaa gggcggggac gtaggtaaaa
     3961 agcagcaagc aggccaccat tgctaaaaaa gaggggatga tctcttttga gatgctttct
     4021 atgctttccc ccgatatggc ggacgcgacg aagaggttta taccgtatgg cggggagatg
     4081 aagccgatcg ccaagttgac ggttattatc agcccgaagt gaatcgtgtc gatgcctatc
     4141 atgcggacca cgggcagtag tatgggcgtc agaatgatga ccgccgggat attatccacg
     4201 aagcagccca ggaccagcag aaagacattt atgatcatca tcagcaggaa ggggctgtcc
     4261 acaaagttaa ccagaagggc cgctatcttc gcgggtatct gcgccatcgc caggtaactc
     4321 gcgaaggcca ttgaaagccc tatcaggaag gacgtggcgc cgtttatcag tcccgtagat
     4381 ttaagacagt cgtaaagggc tttcatatcg agttcgcggt aaacgaactt gcctattatg
     4441 acggaataga cgaccgcgac caccgcggct tctgtgggag tgaaaacgcc cccatagatc
     4501 cccccgagta ttattaccgg gacgagcagg gcccagacgg cctctttgaa ggccttcgat
     4561 acctccgaga acttgggacg ctcggtcatg gaaatgtaat tgcgtttcct ggcggtgaaa
     4621 taacacaccg acatcaaggc gagaccgatt attatgccgg gtattatgcc cgcaatgaac
     4681 atatcaccaa tggaacactg ggcgacgacg ccatagatga cgaaggggat acttgggggg
     4741 atgatgaccc caatcgtacc cgccgccgca gtgatggccg cggcgaaacc ggcgtcatat
     4801 tttctttcct tcatcgaagg gatcatgaag gagccgatgg cggacaccgt cgcgggtccc
     4861 gaaccggaga tcgctgcgaa gaacatgcag gccaggacgg tcaccatggc caggccgcct
     4921 acgatgaacc cgacaaggct ttccgccaac gctaccaggc ggcgtgaaat gccgccggtg
     4981 cacatcaagg ccccggcaag gataaagaac ggtatcgcca gcagagggaa tgagtccagc
     5041 ccagtaacgg ccttttgagc gatcatcagc atagggatat cggaagtgag catcatcgcg
     5101 atgcacgtag acaaccccag ggttattcct atggggatac ttactattat cgtgacgatc
     5161 aggatggaaa aaagaattat agcttccatg gcggcctcta ttccggtatg tccacgacgg
     5221 gatcggccgg cttgggcagc ccgcgtttat gaaggtcgta catttcaacg atcaatcgta
     5281 ggcacatcaa ggcgctcgcc accggaacgg aggcgtaggg ccacgtcatg ggcatactca
     5341 tcgccgtcga tatctggccc gtttcctgga ggaaaatgag aagtttcgtc ccctgccagg
     5401 caaggaagaa gcagaatgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5521 ctggaggaaa atgagaagtt tcgtcccctg ccaggcaagg aagaagcaga atgaaaacca
     5581 ggccagcaag ataatatatt caaaaaatat ccgtgttttc cctttgatca tgtttacgaa
     5641 catctcgacc ctgaaatggc ttctttccct cacggcataa ctggcaccca tccaggaaag
     5701 ccagaggaag atgaaacggg ccagttcttc actccaggag agggaattgg agaacacata
     5761 cctcatcacg acctggaaaa agaccaatac cgtcattacc gccatgatcc agacacagaa
     5821 gtactcctcg aaattatgaa ggaattttct taccgtcatc atacgacccc tttctgacgg
     5881 aaaaagccgc tcccgccctg ccgggcggga gcgggggtac ggaaaccagg gctatttccg
     5941 tacttgtttc agtatttcca taacctcctc gccgaaatcc ttttcgaaca tgggatagac
     6001 cttttcagcg gcatccacga agggttgttt ctcctcgggt gtaagcgagt tggcgatcat
     6061 tcccgccttg agtacttcat ccttgaaagc ggcctcttcc tcatccatgt attcacgctg
     6121 tttcaaagtt gcttccctgg ctgacttgac caccagcgcg ttgagatcgg cgggaaggtt
     6181 gtccatgaac ttcttgttgg ctatcaggac ctcgaaggaa aatacgtggc ccgtctcgga
     6241 aacgtacttc tggacttcgt aaaacttgcc atcgacgatc atggcatagg ggttctcctg
     6301 ggcgtcgacg gtcccttgct gcagggcggt gtagagttcc ccccagctca tgggcgtggg
     6361 attggcgcca acggctttga agaacgcaag gtgtatcggg ttttccatag tgcggatctt
     6421 tatccccttc agatccgccg gggtcttgac gggtcccctg ttgttggtca cgtggcggaa
     6481 tccgttctcc tggaaaccca ggatcagcat acccttggcc gccaggtagg aattgagttt
     6541 ttccccaagg ataccgtcgg ctgcctcgaa ggcagcctgt ttcgtcgtga aaaggtaggg
     6601 taattccagt acctggactc tcttgtcgaa tcccgcgagc ggcgcggagg cggggatagc
     6661 catctggatg gtccccatct gcacgccttc cgagatctcg cggtcgccac cgagctgggc
     6721 gttggggtag agttcgacct tgatcctacc ctgggagcct tcctcgatgg gcttcacaaa
     6781 atactccttc atggccctca ttgtagggtg cgagtcggag ccgatgtagg caaccttgat
     6841 cgtatattcc ggggctgcga aggctgttcc ggccattacc gcgaccaccg ccattaccgt
     6901 aaagaccgca agaagctttc tcaccgctct ttcccccttt tatttattac cttttttata
     6961 gaaacgccct tggggcgtcg ctatcgaagg gccgctacgc ggcttccttc cttaccctga
     7021 cctccccctt ctcggcgtcg acctctacat ggtctccgtc ctctatcact tcataggggt
     7081 cggcctccat ccggtccacc atggtgcatt cgaccatgac ggcggccgaa acgatgaccg
     7141 gctccgtctc aactagtatt atggccgccg gcaggttatg cttcatccaa agctggtaga
     7201 aaccgtccac ctgtaccacc gagctgccct tgccggattt gagcaccagt atcttgttcg
     7261 cgatgctctt gccgtggatg gcgtgattcc tctcgatcac ttcgcccgtc tcgggatctg
     7321 tcaggtaaaa gcacatggca tctctggaaa cgaccgcctc acccccggcg catcccttga
     7381 ctatcttgcg gcacttatag gttttcacat cctgagacat cagtctatcc tcccttttat
     7441 cgctgcctcg acgcattccg acagtctgcg gatggcgaaa ggctgtccac ggcgctccct
     7501 gtagtatgcg cacttcgggg agtccgtaag gccgaggtgc ccttcaaagg atttccagca
     7561 gggttcatcg atgcaggtgt cccccaccat tcgaacccca agaagctcaa gcttctggcg
     7621 aaggtgcgag cgttcggcca gctcgacggc gcccgcttcc gccaggatcc agaaagcggt
     7681 attatggctg accttccttc cggcgaagag cctgtcgatc tccaggatct ggtcgtaggt
     7741 gtagtggggg catcccagca tgaccaggtt gatatcgccc gtagcggggg taagcttggc
     7801 ttccctgtct ttcaggtctt tatcggtgat cacggccgcg gcggccggca ccttcccgcc
     7861 accgaaggca gcctcaatgg tgggcgcctc gggagtgacg ccgaccatgt ggtacatggc
     7921 aacggcgccg ctcgtggcgg cctcggcccc gaagtagagg tagtcttccg gcccggggtt
     7981 gaccttgtgc ttgaaacgaa ggacggggtt atgatagccc gccagttcac ccacgacgtg
     8041 cccaagaatc ccccagtcgt agcagtcctt cacatcggcc tcgacctcga tcaggagatg
     8101 gccgtgcctg ttctcatcga tgtgaaaacc gtaatagggt gttttaccta tcaccgccgc
     8161 ggccaaggcg cttatggaag actcccggtt ggagcgggcg ccgagggcgc cgttgacgta
     8221 gggtgttgcg ctggattctg aataggcgat gtgttcgccg aaaaagggta cgcccgtgct
     8281 cagccctggg gcgcagtggt aggtgagttt tgcacctatt ttgcggtagg catcgccggt
     8341 acggttggcc aggtctaact cttccttggt cacttcatag aatttcgtga ggtagcaggt
     8401 gtcccagtag gggttcgtcg tcggtgtgat ggcacaggtc gcgccgccgc ttacgaggag
     8461 ttcacaccag taggtgtcgc cttcctgccc gctcagcgcc acgtgagcgc gcttgacggg
     8521 tatcagtttt tccgcgtcga aggtgagccc gagggcgtag agcatttcca tcgccttctg
     8581 ctttcccgga ccctcggacc cgtccagtat ccgcttctct tcgtctgtga gaaacatggt
     8641 cagccgcctt tctttattgt gatctgccta tgatgggcag cgagatccca cccatcggga
     8701 taatgcctat cgttgcgccg gggatgagct ccagggctct gtccagggcc tcctggactg
     8761 tagggaaggg cgtgtactgc atggcggcga tgtcctcctt ggacaggcct tcagtgacgg
     8821 aaaagatcct cgccttgttc ctggcgcggc agttgcatat ggcgagtacc gccgatacca
     8881 tatcggcttc cacgtcctcc ggcgaggccg ctcgcagcct ccgtagcgct tcgtccaggg
     8941 ggagggaaag ccattctctg aagagggggt ggttatgggc gagaccctca ccacagggcg
     9001 cggcgaaaat gatgacaccg ccctttttca cggcgaaata ggcgcagctg acgcccttag
     9061 tggcttgcca atagtccata tcggaaggcc acgaactcac tatcactata tccgcgggct
     9121 cgggcaccgt gatcctgaag gacttttcgg cgagctttat tccctccctg tgagccttaa
     9181 tgaagtcgcc ggagaaaact ccggcgatct cattccggta gttttttaca atattgacga
     9241 tgaaagcgag cccggcgatc ctcgccactt cctctatccc ttcgcggcag gggttaccgt
     9301 cggccactcc caggggtata tcgggacaaa aaccggcggc ggcgtgggtg gcggaggtgg
     9361 tgacaaagtc gcagaccccc ggctgcagga tcttggctcc cccggcataa ccggcgtcac
     9421 aatggggcac aatgttccct ataccaacga ggaggtccga ggccaaggcg tagcggttaa
     9481 cgtgggcggg tatctcataa ttccccgcca tgaccgttcc gatatccacc atatcttccc
     9541 tggccgtcgc atcgtgttgc acgaccttga aacgcctgac catttcgggc ccaagttttt
     9601 caaggatctc gccgtcggtc atgagcctgt gggtccccgg ggcggtgagg aaactcatcg
     9661 cgccgtcggg gactccgttt tcgttgagat accccgtgat cacgggcatt atgatgtcca
     9721 ggggcgtact acgagtgcta tcctccacaa ggaagacgat gttcttcttc ccgcgggcga
     9781 gctctttgag gggcggcgtg ccaaggggag at
//
LOCUS       DAOSRG010000032         9476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6966_length_9476_cov_8.828574, whole genome shotgun sequence.
ACCESSION   DAOSRG010000032 DAOSRG010000000
VERSION     DAOSRG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_9476_cov_8.828574"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLM30_02330"
     CDS             complement(<1..550)
                     /locus_tag="PLM30_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HOR54038.1"
                     /translation="MEHYLESLLSNSIFLLSVAVFTGLVLGNLKIRGVGLGTSGALFT
                     GIIMGWLGVKVPSVFFMWNLLIFVTAVGLLSSKDIVGVIKRYGYRFVALSVAVTAAGA
                     LATYFMALLFSGSVEPLLVGGTYTGALTSSPGLGAALEVSGGNPLITIGYTIAYPFGV
                     VSVVLFVQLIPALLKLDLVRERE"
     gene            complement(585..1337)
                     /locus_tag="PLM30_02335"
     CDS             complement(585..1337)
                     /locus_tag="PLM30_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOR54039.1"
                     /translation="MNSYEKLIRILKRFGDPPYEMGITELSKEMGVSKSGIHKIIRSL
                     VRESLLVQNPSTRKYSLGPLVFRLGNVYSDSKGIWDVSHTIMHSLADRTGASVLIGIR
                     EGDDPILAYKLGIGDKYIYQGQTGSRFPFNAGALGKLLTAFLPEDRIRRILKTRNIEK
                     KTPYTETDPEKLMEEYWKIKKQGYAVSIEENLIGAFGMAAPIRDRHGNVWACLSLVGP
                     KETFTPDVVDLWLSLLRNGADEISSRLGFRGV"
     gene            complement(1505..2206)
                     /locus_tag="PLM30_02340"
     CDS             complement(1505..2206)
                     /locus_tag="PLM30_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOR54040.1"
                     /translation="MRRTKRTVTVVLILSVFLLPLLSTPLLAATANERITSSVHVVRD
                     MSREEDVSAMADLVKSAKGVVIVPNYVKAALGIGGAHGEGLILRRDASSNTWYGPNFL
                     SITGASIGLQIGVQSTALLLVITNQKGMDRFYGDKFKLGADIDIAAGPVGRSTGAATD
                     VNLQASIYSYSINKGLFAGLSLSGAAVTTDHDANASYWGSSINSKEILQKKATAAGIQ
                     PLVSALTELIKKAVD"
     gene            complement(2294..2569)
                     /locus_tag="PLM30_02345"
     CDS             complement(2294..2569)
                     /locus_tag="PLM30_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOR54041.1"
                     /translation="MKCSDGTYYCGIAQDPQKRLRQHNEGKGARYTASRRPVELVFAT
                     SRGYSRAQAQAIERKAKAKPKTLKRSFLEAIDAQGGNADPPSGDPSR"
     gene            complement(2595..3710)
                     /locus_tag="PLM30_02350"
     CDS             complement(2595..3710)
                     /locus_tag="PLM30_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54042.1"
                     /translation="MKIEEFKLERTLARFQNEVEYDLSASGIYPMFIREILSPVEMED
                     AYNNLHLKYVHTAGTEHLRKAVASFYEGMDPSSVFMTNGSAEALFLIAWGLIDPGDEV
                     AFMIPNFMLLSHVVESNGAVVRNFNLDPGRGWSLDLDSLKRAVTPKTKLVCVCNPNNP
                     TGTILSREQMEQIVEVASGAGAYLLSDEVYRGAEQTDEMTPSFWGMYDKTIIISGLSK
                     SFGLPGLRIGWVAGPRGVAEMSWFYHDFLSTTVTTFSDYLATKALEPAMREKIFARNR
                     GIVRQNLATFTEWVESHHGVLSFTPPAAGCFAFVKYDLPVPSWDLVMDMVENNSVFVI
                     PGSCFGIENHLRMNFGVDRPRLVEGLKRVDRTLSKYL"
     gene            complement(3790..5037)
                     /locus_tag="PLM30_02355"
     CDS             complement(3790..5037)
                     /locus_tag="PLM30_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOR54043.1"
                     /translation="MSWRIHPEGKGDKRKEQGYKTSLWSHRILLARVYLRSLPFYHSR
                     RVSSPVIPRRPTWGTFMDWGLNFFLLFIIGIPGWLLFRAFRIPAAVMVGAMVSAAFFA
                     VKGWVVPATPEGANLLFQVILGLFVGIHFTGRAGRDLVSSLRVALLAGVWWISFPLGL
                     GWVVNNWFDLGLATSILGTVPGGIAEMSMLALTLEADAALVALMQFFRLSSVLIAMPV
                     ISTRMGKAPAGAAGGDPDPGNGRKDASPRPGEPGPRPGSRALTLSIAVAGGLLFYFLG
                     VPVGGFVGSMIFTAAAAASGLPIRPLPVMVRHVAQLGLGSLIGLYATPGTLATLYCMF
                     LTIVGTTAAMLAWGIMLAYIVRAVTRWNLMTCLIATCPGGITQLASIAEDLGADPLKV
                     SLLHLVRLLTIYVILPPLIKALV"
     gene            5077..5499
                     /gene="msrB"
                     /locus_tag="PLM30_02360"
     CDS             5077..5499
                     /gene="msrB"
                     /locus_tag="PLM30_02360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOR54044.1"
                     /translation="MSQEKITRTEEEWKSLLTPEQFRVTRGGGTEPPFSGKYYNHREN
                     GTYLCSNCGNDLFTSDSKYDSGSGWPSFFEPLSPESVSTREDRSHGMTRVEVLCARCD
                     AHLGHLFPDGPPPTGMRYCINSVALDFRPDQPPRPGTD"
     gene            complement(5513..6691)
                     /locus_tag="PLM30_02365"
     CDS             complement(5513..6691)
                     /locus_tag="PLM30_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR54045.1"
                     /translation="MEILISLSVVLLAGFLLTRLTKLAKLPDVTGYIIAGILIGPHIL
                     KLVPPRMIEDMGFISDIALAFIAFGVGRFFRKEVFRETGFGVITITLMESLLAGVIVT
                     LSLHYLFHLDWDFSLLLGAIGTATAPASTTVTIRQYHARGNFVNILLQVVALDDGVCL
                     IVYSIALAIVNAGDGAGVCVSEIVLPIIFNILALALGFVGGFILSRLKAPKRSDDDRV
                     ILTISLLLGIAGLCVAVDISPLLSCMIFGTTYVNLAKDRELFKHLDRLSPPIFSLFFV
                     VSGMSLDLSTLGSMGVIGASYFVIRILGKYIGAYLGCLMAKTPRNVRNYLGLALIPQA
                     GVAIGLAFLSKRVLPEATGHMLLAIILSSSFLYELIGPVSAKIALIYSGSIQMEQIEE
                     "
     assembly_gap    6942..7041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7136..8053
                     /locus_tag="PLM30_02370"
     CDS             7136..8053
                     /locus_tag="PLM30_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOR54046.1"
                     /translation="MRIGEMIDPKLYSLLAVVEFGSYTRAAEHLSLTQPAVTHHMKQL
                     EKELDIRIFNRIGNRIRPTNEGQILIGYARRNIALYEAMNQGILDEKKHFRRLTVGIT
                     PTAESNAAAEVLGEYSARNPGTRITIITDYINNLYSMLKNYEVDLVVSEGKVQDESIN
                     SLLLDTDSLVLVLSNNHPLARKSMITVKELKELPLILRSASSDTRNLFRAHLESHNMS
                     LDDFNVILEIDNIGTIKNLVRREIGVSILSRSVCLDELKKGSLSVLPIENLSMVREIN
                     ILYHRDFRHEDLLQTILKEYNKAIRNYAV"
     gene            8284..>9476
                     /locus_tag="PLM30_02375"
     CDS             8284..>9476
                     /locus_tag="PLM30_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54047.1"
                     /translation="MGKSFIGKCLLLVLAALLVVGTLSGVALAEEKVIKIGTLFPLTG
                     SVALAGQRCRAAVETAAEVINNAHPELKVPLAAKAGLLDGYKIVLIHADHQGKPDVAK
                     SEAERLFDQEKVWTIIGCYNSACTKPASFVAELKQKVFMCGCSSSGALTERDFKYFFR
                     MAPTDKTESLEFVEFIEYVNGKKNAGVKTVGLIYENTEFGKHAAEEGRMAAEASGFKI
                     VADVAFNPGATNLNSEVQTLKAANPDAVFGACLGGDYTLWVRTMKQMDWLPKVALNYC
                     TGYQDPIITGQLGDDANYFMGGTGYAPEFAELMPAVAAVEEIYKAKTDPSVPFDSDSI
                     QEAVALFIVAQAIEKSGSLDPTVVAETLYANEWESPLSMGGKVAFSPGGQNVKAMSML
                     TQLQGG"
BASE COUNT         2068 a         2728 c         2732 g         1848 t
ORIGIN      
        1 tctcccgttc ccgcacgagg tccagcttga gcagcgcggg gatcagctga acgaagagca
       61 ccaccgagac caccccgaag gggtaggcga tggtgtagcc gatggtgatg agcgggttcc
      121 ccccggagac ctcgagggcg gcgccaaggc cgggggaact ggtaagggcc ccggtgtagg
      181 taccccctac aagaaggggt tcaacggaac ccgagaagag aagggccatg aaataggtcg
      241 ccaacgcccc ggcggcggtc acggccacgg agagagctac gaaacggtag ccataccttt
      301 tgatgacccc gacgatatcc ttggacgaca ggagcccgac ggcggtgacg aagatcagca
      361 agttccacat gaagaaaacg ctgggcactt taacgcccag ccaccccatg attattcccg
      421 tgaaaagagc cccggaagtc cccaggccca ctcccctgat cttcaggttg ccgaggacga
      481 ggccggtgaa gacggctacg ctgagaagga atattgaatt ggaaagcagt gactcgagat
      541 agtgctccat gacttccgag cccgtccctt ctgtaatgag ttctctacac gccccggaat
      601 ccaaggcggc ttgatatctc gtcggcgccg ttcctgagga gtgaaagcca cagatcgacc
      661 acgtcggggg tgaaggtctc cttgggaccg accaggctga ggcaggccca cacgttccca
      721 tgccggtccc ttataggggc ggccatgccg aaggctccga tgaggttctc ctcgatgctg
      781 acggcgtagc cctgcttttt gatcttccag tactcctcca tcaacttttc agggtcggtc
      841 tcggtatagg gtgttttttt ctcgatattc ctggtcttga gtatcctccg gatgcggtct
      901 tcaggaagga aggccgtcag cagtttcccc agggcgccgg cgttgaaggg aaagcggctc
      961 ccagtctggc cctggtatat gtacttgtcg ccgatgccca acttgtacgc caggataggg
     1021 tcatcgccct cccggatgcc gatgaggacg gaagcgcccg tccggtccgc cagggagtgc
     1081 attatggtgt gggagacgtc ccagatgccc ttggaatcgc tgtagacatt accaagacgg
     1141 aagacgaggg ggccgaggga gtacttcctc gtagaggggt tctgtaccag caggctctca
     1201 cgcacaaggg acctgatgat cttgtggatg ccgctctttg agacgcccat ctccttcgag
     1261 agttccgtta ttcccatctc gtaggggggg tcgccaaacc ttttcaatat ccggatgagc
     1321 ttttcgtaac tgttcatttc ccatctccgt tagctggagg agaactgatt tctccccgga
     1381 agaacctttc tgtcattttt ccggacaaaa gtctttttgt caaggattag tcagatgacg
     1441 cggtccccgc caaaagggaa caaaaaagcg ggaccgaggt cccgcccgac aaaaactgca
     1501 atgcctaatc gacggctttt tttatcagtt cggtcagcgc tgaaaccagg ggctgtatac
     1561 cggccgctgt tgccttcttt tggagtatct ccttcgagtt gatcgatgac ccccagtagg
     1621 aagcgttggc gtcgtggtcg gtggtgaccg ccgccccgct gagggaaagt cccgcgaaga
     1681 gtcctttgtt gatggaatag ctgtagatcg aggcctggag gttcacatcc gtcgcggccc
     1741 ctgtgctacg gcccaccggg cccgcggcga tgtcgatatc ggctcccagc ttgaacttgt
     1801 cgccgtagaa ccggtccatt cctttctggt tggttatcac caggaggagg gccgtggact
     1861 ggacaccgat ctggagccct atggaagcgc cggtaatgct caggaaattg ggcccgtacc
     1921 aggtgttact ggatgcatcg cgcctcagta tcaacccctc cccgtgggcc cccccgatcc
     1981 ccagggcggc cttcacgtag tttgggacga tgacgacccc cttggcggac ttgacaaggt
     2041 cggccatggc gctgacgtct tcctccctgg acatatcgcg gaccacgtgc accgaagagg
     2101 ttattctctc gttggccgta gccgccagga ggggcgtcga aagaaggggc agcaggaaca
     2161 ctgacaaaat caagactacg gtcactgtgc gtttagttct tctcattacc gccatctcct
     2221 tcgcttttta tctgccgaga ctgtacttta acaggttatc atattttcag aggcccttca
     2281 ctggcagagg gcctcatcgg gagggatctc ctgagggcgg gtctgcgttc cctccctggg
     2341 catcgatcgc ctccaggaag gaacgcttga gggttttggg cttcgccttc gcctttctct
     2401 cgatagcctg ggcctgggcg cgactgtacc cgcgggacgt ggcaaagacc agttccaccg
     2461 gccggcggga agcggtgtac cgggcacctt ttccttcgtt gtgctgccgg aggcgtttct
     2521 gtgggtcctg ggcgatgccg cagtaatagg tgccgtcgga acacttcaaa agataaacgt
     2581 gaaaaaggcc cggatcagag gtactttgac agggtcctgt caaccctctt cagcccttcc
     2641 acgaggcgcg gcctgtcgac gccgaagttc atcctcaggt ggttctcgat gccgaaacag
     2701 ctgcccggta tgacgaagac gctgttgttt tccaccatgt ccatcacgag gtcccaggat
     2761 gggacgggga ggtcgtactt cacgaaagcg aagcagcccg ccgccggggg ggtgaaggaa
     2821 agcacaccgt ggtggctttc aacccattcc gtgaaggtgg cgaggttctg ccggacgatc
     2881 cccctgttcc tggcaaagat cttctcgcgc atggccggtt caagggcttt cgtggcgagg
     2941 tagtcgctga aagtggtgac cgtcgtggac aggaaatcat ggtagaacca ggacatctcg
     3001 gctacgcccc tgggccctgc cacccacccg atcctgaggc ccggcagacc gaaggacttc
     3061 gataggccgc tgatgataat ggtcttgtcg tacatgcccc agaaactcgg ggtcatctcg
     3121 tcggtctgtt cggccccgcg atacacctca tcggagagaa ggtaggctcc cgcccccgag
     3181 gcgacctcga cgatctgctc catctgctcc cgggagagga tcgtcccggt ggggttgttg
     3241 gggttgcaga cgcaaacgag cttggtcttg ggcgttacgg cccttttcag gctgtccagg
     3301 tcgagggacc agcctcgccc cgggtcaagg ttgaagttcc gcaccaccgc cccgttggac
     3361 tccaccacgt gggataggag catgaagttg gggatcatga aggcaacctc gtcgcccgga
     3421 tcgatgaggc cccaggcgat caggaagagc gcctcggccg agccgttcgt catgaaaacg
     3481 gaggaggggt ccatcccttc gtagaaggaa gctacggcct tcctgaggtg ttccgtgccg
     3541 gcggtgtgga catacttgag gtgcaggttg ttgtaggcgt cttccatttc cacgggtgag
     3601 aggatctctc gtatgaacat ggggtatatt ccggaagcgc tgaggtcgta ttccacttcg
     3661 ttctggaatc gggccagcgt tctttcgagt ttgaattctt cgatcttcat ctaataacct
     3721 cctgtctcac tgaggggatg ccccttcccg gattttatcc caaatctcca ggagaaagaa
     3781 gaaaaccttc tagaccagcg ccttgataag aggggggagg atgacgtaaa tggtcaaaag
     3841 cctgacgagg tgcaggagac tcaccttgag ggggtcggct cccaggtcct cggcgatgga
     3901 ggccagctgg gtgatccccc ccgggcaggt ggctatgagg caggtcatga ggttccaccg
     3961 ggtgacggcc ctcacaatgt aggccagcat gatgccccag gcaagcatgg ccgctgtcgt
     4021 ccccacgatg gtcaggaaca tgcagtaaag ggtcgcaagg gtcccggggg tggcgtaaag
     4081 ccctatgagc gagcccagcc ccagttgggc cacgtgcctc accatcaccg gcaggggacg
     4141 gatgggaagc cccgatgcgg ccgcggcggc ggtgaagatc atggagccca cgaaaccacc
     4201 gacgggaacg ccgaggaagt agaacaaaag gcctcccgct actgctatgg aaagggtcag
     4261 ggcccgactg ccgggccgcg gcccgggttc gcccggccgg ggcgaggcat ctttccgccc
     4321 gttccctggg tcgggatcac cccctgccgc gccggcgggg gccttgccca tcctggtgga
     4381 gatcaccggc atggcaatga gcaccgagga gagccggaag aactgcatca acgccacaag
     4441 ggcggcgtcg gcctcgaggg tgagggccag catgctcatc tcggcgatgc ctccgggcac
     4501 ggtcccgagg atggaggtag ccagacccag gtcgaaccaa ttgttaacca cccaacccag
     4561 gccgaggggg aaactgatcc accagacccc ggcaaggagg gccacacgca gactgcttac
     4621 gaggtccctg ccggcccggc cggtaaaatg gatcccgacg aagagcccaa ggatcacctg
     4681 gaaaagcaag ttcgccccct cgggggtcgc cggcaccacc caccccttga cggcgaagaa
     4741 ggccgccgag accatggccc ctaccattac ggcagcgggt atccggaagg cccggaaaag
     4801 cagccaccct ggtatcccga taatgaataa aagaaagaag ttcaagcccc agtccataaa
     4861 agtcccccag gtcgggcggc ggggaataac cggggaagaa acccgccggg aatgataaaa
     4921 tggtaaggat ctcaaatata cccttgcaag gagtatccga tgggaccaaa gggacgtttt
     4981 atagccctgt tccttgcgtt tgtcgccttt tccctctgga tgtatccgcc aagacaccaa
     5041 cgcgggtcgc caggggatct caaggggggt gccgtcttgt cccaggagaa aataacccgt
     5101 accgaggaag aatggaagtc tctcctcaca cccgaacagt tcagggtcac caggggaggt
     5161 ggaacggaac cgcccttttc aggaaaatac tacaaccaca gggagaatgg aacctacctc
     5221 tgctcaaact gcgggaacga cctgttcact tcggactcaa agtatgactc cggaagcggg
     5281 tggccgagtt tttttgaacc cctgtcgccc gaaagcgttt cgacccggga ggaccggtcc
     5341 cacggcatga ccagggtgga agtgctctgc gccaggtgcg acgcgcactt gggccacctg
     5401 ttccccgatg ggcctccccc gacgggtatg cgttactgca tcaactccgt cgcccttgat
     5461 ttcaggcccg accagccccc tcgccccggc acggattgag gccgtccggg ggtcactcct
     5521 ctatctgttc catctggatg gagccggagt agatcagggc gatcttggcg ctcacgggcc
     5581 cgatcaattc gtacaggaac gacgaggata atattatcgc cagtagcatg tggcccgtgg
     5641 cttccggcaa gaccctcttg ctcaggaagg ccagccctat agcgaccccg gcctgcggga
     5701 tcagggctaa cccaaggtag ttccgcacat tcctgggggt cttcgccatg aggcaaccca
     5761 ggtaggcgcc gatgtatttc cccaggatcc ggataacgaa gtaggaggcg ccgatgaccc
     5821 ccatagagcc cagggtgctc aagtccagac tcatccccga taccacgaag aacaacgaaa
     5881 agatcggcgg gctcaacctg tccaggtgct tgaacaactc cctgtccttc gccaggttca
     5941 cgtaagtggt cccgaagatc atgcacgaca gcagcgggga aatatccacc gccacgcaca
     6001 ggccggcaat gcccagcagc agggaaatgg tcaggatgac acgatcgtca tcgctacgct
     6061 tcggggcctt cagcctgctc agtatgaacc cgcccacgaa gccgagggcg agggcgagga
     6121 tgttgaaaat aatgggcaat actatctcgg agacacagac cccagcccca tcgccggcat
     6181 tgacgatggc cagggctatg ctgtagacga taaggcagac gccgtcgtcg agggcgacca
     6241 cctgcagcag tatgttcacg aaattgccgc gggcatggta ctggcggatc gtaaccgtcg
     6301 tactggcagg ggccgtcgcg gtcccgatcg cccccagaag caggctgaag tcccaatcca
     6361 ggtggaaaag ataatgcaat gagagggtca cgataacccc ggcgagcagc gattccatca
     6421 aggtgatggt gatgacgccg aagccggttt ccctgaagac ttcctttctg aaaaagcgcc
     6481 caaccccgaa ggcgatgaac gccagggcga tatcgctgat aaaccccatg tcctcgatca
     6541 tccttggtgg gaccagtttc aggatatggg ggcctatcag gatcccggcg ataatgtagc
     6601 ccgtgacatc gggcagcttc gccagcttgg tgaggcgcgt cagcaggaag ccggcaagca
     6661 gtaccaccga cagggaaatc agtatttcca tcgtgtggct tgatctgcac agaagatccg
     6721 ccatctctta cactccaaaa agcaggatac cccgatgatc ttcccggcgg ccgcattgtt
     6781 ttcaacaacg cgcgaaaaaa gtagaataca tggataataa caaaaaacgg gccttcggca
     6841 ttacgtcgcc gggtctcggc ctcccgaccc ctaccgcaag gtttccttgc cttcttaatt
     6901 tagttaggct atcattctaa tcgcaaaact aataagtctt tnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nttgccttct taatttagtt aggctatcat tctaatcgca
     7081 aaactaataa gtctttttta tcttttccgg tttttctata agttatcttt atagagtgag
     7141 gataggcgaa atgatcgacc cgaaacttta ctccctttta gcggtggtcg agttcggcag
     7201 ttacacccgg gcggcggagc atctctccct gacccagccc gccgtgaccc atcacatgaa
     7261 gcaactggaa aaagagctgg acatcaggat cttcaatcgt atcggcaaca ggataaggcc
     7321 gacgaacgaa ggacagatcc tcatcggtta cgcccggcgt aacattgccc tgtacgaggc
     7381 gatgaaccag ggcatcctgg atgaaaaaaa acatttccgc cgacttacgg tggggatcac
     7441 gcccactgcc gagagcaacg ccgccgctga ggttttgggg gaatacagcg ccaggaaccc
     7501 gggaacaagg ataacgatca ttactgatta cattaataat ctttatagta tgctcaagaa
     7561 ttacgaggtg gacctggtcg tttcggaggg taaggtacag gacgaaagca tcaattcgct
     7621 tttgctggat accgattctc tggtgctggt cctgtccaac aaccaccccc tggccaggaa
     7681 aagcatgata acggtgaaag agttgaagga actgcccctg atcctccgga gcgcgtcctc
     7741 cgataccagg aaccttttca gggcgcacct ggaaagccac aacatgtccc tcgatgactt
     7801 caacgtcatc ctggaaatag acaatatagg gaccatcaag aacctggtaa ggcgcgaaat
     7861 aggggtctcg atactctctc gcagcgtgtg cctcgacgag ctcaagaagg gaagcctttc
     7921 agtgttgccc atagagaacc tgagcatggt ccgggagatc aatattctgt atcaccgcga
     7981 tttcaggcat gaggacctgt tgcagactat ccttaaggaa tacaacaagg cgataaggaa
     8041 ctatgcggtg tgatacaggg cctcgctccg gcggcagggt cccggggagt gcggttttca
     8101 cccttcccac ccaaggatga cctttcacct gtcgaaaaga gatcctacac tgtccggcca
     8161 cacatgtttc attaagcgcc ttgacgcttc tcaaccgctt cacttaaaat caaggggcgg
     8221 acgaaatttt gaacaaatta gtccttgcgg tcacgaggac atcaccaaga aggaggagtc
     8281 gatatgggga agagctttat cggtaagtgt ctgcttcttg ttcttgcggc gctccttgtc
     8341 gtcggcaccc tgtccggggt cgccctcgca gaggagaagg tgatcaagat cgggaccctt
     8401 ttcccgctca ccggttcggt ggcccttgca ggccagcggt gccgtgctgc cgttgagacc
     8461 gctgcggagg tcatcaacaa cgcccacccc gaactgaagg tacccctggc tgcaaaagcg
     8521 ggccttctgg atggctacaa gatagtcctc atccatgctg accaccaggg caaacccgat
     8581 gtggcgaagt ccgaagccga acgccttttc gaccaggaga aggtttggac catcatcggt
     8641 tgctacaaca gcgcctgcac gaaacccgcc agtttcgtgg ccgaactgaa acagaaggtc
     8701 ttcatgtgcg ggtgctccag ctccggcgcc ctgacggagc gcgacttcaa gtacttcttc
     8761 cgtatggccc ccacggacaa gacggagtcc ctcgagttcg tcgagttcat tgaatatgtc
     8821 aacgggaaga aaaatgccgg cgttaagacc gtcggtttga tctacgagaa caccgagttc
     8881 ggcaaacacg ccgccgagga aggccgcatg gccgccgagg ccagcggttt caagatcgtt
     8941 gccgatgtcg cttttaaccc cggggccacc aacctcaaca gcgaggtcca gacccttaag
     9001 gccgccaacc ctgacgcagt tttcggggct tgcctcggcg gggactacac cctctgggtc
     9061 cgcaccatga agcagatgga ttggctcccg aaggtcgccc tcaactactg cacgggctac
     9121 caggacccca tcatcaccgg ccagctcggt gacgacgcca attacttcat gggcggcaca
     9181 ggctacgccc ccgagttcgc cgaactgatg ccggccgtcg cggcggtcga ggagatctac
     9241 aaggccaaga ccgaccccag cgtccccttt gacagcgatt cgatccagga agcggtggcc
     9301 ctcttcatcg tagcccaggc catcgagaaa tccgggagcc tcgatcccac cgtcgtggcc
     9361 gagacccttt acgccaacga gtgggagtcc cccctgtcca tgggcggcaa ggtcgccttc
     9421 tcgcccggcg gacagaacgt caaggccatg agcatgctca cccagctcca gggcgg
//
LOCUS       DAOSRG010000033         9469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_6972_length_9469_cov_7.924474, whole genome shotgun sequence.
ACCESSION   DAOSRG010000033 DAOSRG010000000
VERSION     DAOSRG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_9469_cov_7.924474"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLM30_02380"
     CDS             complement(<1..436)
                     /locus_tag="PLM30_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOR54048.1"
                     /translation="MRGKGRLKKVNESEVCGDLLEGRKEYDQAVAKGLSRHPEMKEAF
                     TTGGGIPLERLYTPIDIEGLDYCEDLGFPGSYPYTRGVQPTMYRGRFWTMRQYAGFAT
                     AAESNARYRYLLEQGQTGLSVAFDLPTQIGYDSDHPMARGEVG"
     gene            complement(609..1391)
                     /locus_tag="PLM30_02385"
     CDS             complement(609..1391)
                     /locus_tag="PLM30_02385"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOR54049.1"
                     /translation="MAVSVDLNSDLGEGFGAWGMGDDALIMDHVTSVNIACGFHAGDP
                     EIMIATVEGALRKGIALGAHPGYPDLAGFGRREMGCTPGEIYAFCLYQVGALSAIVRS
                     RGGRLRHVKAHGALYNRAAGDEAAAGAIVCAARDLGEGLIIVGPQGSCLEKACREEGL
                     PFAAEAFADRAYTPGGYLVPRSAPGSVIEDPREAASRAVRMVLEGKVTALGGEDILLR
                     ADTICLHGDTPGSAGMAALVRSSLEEAGVRVLPLDAFLRGAP"
     gene            complement(1391..2458)
                     /locus_tag="PLM30_02390"
     CDS             complement(1391..2458)
                     /locus_tag="PLM30_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOR54050.1"
                     /translation="MSLEVQSPGLLTTVQDLGRSGFRAFGMPLSGALDPFSLAAGNLV
                     VGNGPGAAGLEVTLVGPGLAFRSERLVCVTGGDLSPRVNGKAIPCWEGVLLREGDVLT
                     FGGAGPGGARCWVCVGGGIDTPPVMGSRSTYLRGKLGGYKGRRLEKGDILPLGVPDIL
                     SPRGEGFAVPGELRPGFIEGPVVRVIPGPQEGHFTEEGLEVFYKSSYCIDPGSDRMGY
                     RLRGPAVHHREGADIISDAVPWGAVQVPGNGLPIIMMADGQTTGGYPKIAAAISADLG
                     LLAAAAPGDSLSFRRCGLGEAAAASRALKGSMDGLRVLLAGYRSRPVRSVPKADRGAL
                     RLHFDGGSFDITWEIIGREDR"
     gene            complement(2455..3177)
                     /gene="pxpB"
                     /locus_tag="PLM30_02395"
     CDS             complement(2455..3177)
                     /gene="pxpB"
                     /locus_tag="PLM30_02395"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOR54051.1"
                     /translation="MASRRAGEDAPRLAWCGDACLMAEFGDAIDPRVNSRVLALGVRL
                     ATLAPPWLVETVSTYRSLAVYVDPWEGPRDEIEALVARLALSAKGEPPQPTGTFSVPV
                     CYGGTWGPDLERVSGHTGLEPDEVVRRHSGGRYRVFMMGFTPGFPYLGGLDPALETPR
                     LESPRGLVAAGSVGIAGRQGGIYPIESPGGWNIIGRTPLALFDPGREPPFLMATGMDI
                     RFVPVTAGEFEEIVRGGRGVTP"
     gene            complement(3167..3763)
                     /locus_tag="PLM30_02400"
     CDS             complement(3167..3763)
                     /locus_tag="PLM30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOR54052.1"
                     /translation="MRHASRLVAPLDDEAVRPLRTGDRALISGVILTALDEAHRRMTE
                     YARENRPLPFDLKGQVLCYLEPSPTPLGRVCGPIGPVASGRMDPYTPLLLGLGLKGII
                     GRGRRSPEVIDSIVKNRALYFGAAGGAAVLLSGCVTRVESVAWPEMGPEAVFRMRVED
                     FPVVVLVDARGNDLYRDGPARYRRGGSPPSKEGLTGGL"
     gene            complement(3777..4634)
                     /locus_tag="PLM30_02405"
     CDS             complement(3777..4634)
                     /locus_tag="PLM30_02405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOR54053.1"
                     /translation="MAKVREVEASLVTEAVRGLFFKANVSIGQDVYNALAHARDRLEE
                     SPLGRSVLDQVLRNHDLARDGHVPLCQDTGMAVVFIDLGQEVLLVGGGLVEAVDSGVE
                     EAYREGFFRKSIVTDPLFDRRNTGTNTPAVLHTRIVPGDRVGLLVAPRGFGGENVSAL
                     RVLSPDQGPRELLEFVRDTVEAAGPEPCPPTLVGVGIGGTVEAAAVMAKRATLYPVGR
                     RNPDRRYAELELRTLEAVNALGIGPAGLGGTVTCLDIHMDFAPAHVAGTPVVVNICCH
                     AARHAAVTI"
     gene            complement(4686..5885)
                     /locus_tag="PLM30_02410"
     CDS             complement(4686..5885)
                     /locus_tag="PLM30_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOR54054.1"
                     /translation="MIFLVIYFCPRCGRSFNETSRPVSCDCGTPLKSTVDWADLEIDP
                     GARGMARYSSALPPLDPSVSMGEGWTPLVRSRSGGPYYKLEYLFPTGSFKDRGSVLVM
                     TEAKNQGQESVVQDSSGNAGASIAAYAARCGIRATVIVPRDTAEAKRWQIELYGATLE
                     IVDGDRAAAASVAFSLSGSTYYASHVWNPLFLEGTKTVAFEIWEQLGFRAPDAVVVPA
                     GNGTMLLGVFKGFRELLQRARVRRMPRIYAAQGTQCAPLYAEWNRTEEKTFRATIAEG
                     IAVTAPPRMREMIDALRASRGDVITVDDQDILAEQERLALEEGLLVEPTAAAAPAAER
                     ALRAAGVITAEDTVVVPLTGSGLKKVPPRRPKKQASPDEERPLFETRAKRVGSPVMPG
                     RATGLRR"
     regulatory      5994..6102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6209..6712
                     /gene="thiT"
                     /locus_tag="PLM30_02415"
     CDS             6209..6712
                     /gene="thiT"
                     /locus_tag="PLM30_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOR54055.1"
                     /translation="MESEKVRILVEGAMTAALAVALSYVRIWRMPQGGSITLENVPLF
                     LFALRWGVKAGLGAGSVAGLLQLVLGGYIIHPAQALLDYPLAFAALALAALWRKPLWA
                     GLTLGTSARLLCHVLSGAIFFAAYAPEGSNVWLYSTVYNATFMVPNLALSIVAVYIIW
                     PRLSKRA"
     gene            complement(6769..7014)
                     /locus_tag="PLM30_02420"
     CDS             complement(6769..7014)
                     /locus_tag="PLM30_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOR54056.1"
                     /translation="MNDLVGYLVVGIIAGWLAGKVWQGRGFGFFGNLGVGVIGAFIGG
                     VIFSSIGIYPMGFIGSLVMAFAGAVVLLFVVGLLKGK"
     gene            complement(7052..7762)
                     /gene="pssA"
                     /locus_tag="PLM30_02425"
     CDS             complement(7052..7762)
                     /gene="pssA"
                     /locus_tag="PLM30_02425"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOR54057.1"
                     /translation="MKTLVPNMITCGNMLSGMLSLVLAFHGHFVPASWLVFFAVFFDF
                     MDGKMARKLGGSSSFGMELDSLADVVSFGVAPAMIIYSVYLKGFFGVTGALVAAFFAL
                     CGALRLARFNVHHVDGSFQGLPIPAGGHLLVSFVVAGIHLPPVLMALLVTAAGFLMIS
                     SVPFGNLKALKAGSLNKRKAVFMWASAFMLIAVLRNKAPLAGMLIYIVSGFAGVDWGK
                     WLSLEETVGEEDLAEEDT"
     gene            complement(7797..8414)
                     /locus_tag="PLM30_02430"
     CDS             complement(7797..8414)
                     /locus_tag="PLM30_02430"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOR54058.1"
                     /translation="MLAPDGKRTVLFFAGAAVLAALTLGALAGCLLGAAAVFLAWFFR
                     DPERTTPEGSEAWVSPADGRVTEIRPSEHPFTGKSTVVGVFMSPLDVHVNRMPFDGTV
                     TFMEYVPGKKLMAYNPKASLENERFYMGFQTRHGRGMTVQVAGFLARRITCSRKKGDA
                     LARGERFGMIKLGSKVDLYLPEGVSPVVTVGAKVKAGKTAIGVRK"
     gene            complement(8421..>9469)
                     /locus_tag="PLM30_02435"
     CDS             complement(8421..>9469)
                     /locus_tag="PLM30_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOR54059.1"
                     /translation="LDMVVREELREVRRDFADDRRTTIVDAMDEVSIEDLIPEEEIVV
                     VLSRDGFLRRMPLEQYRLQGRGGKGVKGATPKVGDEAAIITVSSTHRDIFLFTNKGRV
                     FALKGHAIPEPRTGKGRLASQFISLENGESVVAMKDRHLRGASFVFFITARGVAKRLP
                     AGELENLTRAGRRVLSLDDGDEIARVRFTSGEDEILMVTAKGQALRVFEDEFRPLGRQ
                     ARGVRGIRLKAGDSVVGCDIVKPGHQVLLVSELGVGKRTAFDEFTAHHRATAGVRAMR
                     ISSKTGLLVGAWGLMDEDQIIVMTNRGRVIRMNVDEVSTLGRTATGYRVVKVAEGDMV
                     ADISVIRSGEGEGE"
BASE COUNT         1615 a         3255 c         2990 g         1609 t
ORIGIN      
        1 tgccgacctc gccccttgcc atggggtgat cggaatcgta ccctatctgg gtcggaaggt
       61 cgaaggcgac ggaaagcccc gtctgaccct gttccagcag gtagcgatac ctggcgttcg
      121 actcggccgc cgtggcgaaa ccggcgtact gcctcatggt ccagaagcgg ccgcgataca
      181 tggtgggctg gacacccctc gtgtaagggt aagacccggg gaaacccaga tcttcgcaat
      241 agtccaggcc ctcgatgtct ataggggtgt agaggcgctc caggggtatg ccccctccgg
      301 tagtgaaggc ctccttcatc tccgggtgtc tcgaaagccc cttggcaacc gcttggtcgt
      361 attccttcct accttccagc agatccccac agacttcgct ttcgttcact tttttcaaac
      421 gcccctttcc tcgcatgagc gggacctaaa ggccgaattg acggaacagt gcttaaaatt
      481 ccggtccctt tgtgaatctt caagcaggta ttgacattat tccctatcat ctcaaaagat
      541 gcaaccattt cattgaattg cgagacaatt ctatccaatt tgccgggaga aagacaaccc
      601 tcgccgcgtc agggggcgcc ccggaggaag gcatccaggg ggagcaccct cacgccggcc
      661 tcctccaggg atgaccggac aagggccgcc atcccggccg agccgggggt gtccccgtga
      721 aggcagatcg tatcggccct caggagtata tcctcccctc ccagggcggt cacctttcct
      781 tcgaggacca tcctgacggc cctcgaggcc gcctcccggg ggtcctctat cacggagccc
      841 ggcgccgacc gggggacgag gtaccctccc ggggtgtagg cccggtccgc gaaggcctct
      901 gccgcgaagg gcaggccctc ctcacggcag gccttttcaa ggcatgaacc ctgggggccc
      961 acgatgatga gcccctcccc gaggtccctg gcggcgcaga cgatggcccc ggccgcggcc
     1021 tcatccccgg ccgccctgtt gtaaagggcc ccgtgggctt tcacgtgccg caacctcccg
     1081 ccccgggacc tgacgatggc cgacagggct cctacctggt agaggcagaa agcgtagatc
     1141 tcccccggcg tgcaacccat ttccctcctg ccgaaaccgg cgaggtcggg gtaaccgggg
     1201 tgagccccga gggctatacc cttccgtaac gccccctcga cggtggcgat catgatctcc
     1261 gggtcgccgg cgtggaaacc gcaggcgatg ttgacggagg tgacatgatc catgataagg
     1321 gcgtcgtcgc ccatccccca ggcgccgaaa ccttcaccca gatcgctgtt gaggtccacc
     1381 gatacggcca ttaccgatcc tcccgtccta ttatctccca ggttatgtcg aaggatccac
     1441 catcgaagtg aagccgcagg gctcccctgt cagccttcgg gaccgatcgg accggccttg
     1501 accggtaacc cgccaggagc accctgagcc cgtccatgct tcctttcagg gcccgtgagg
     1561 cggcggcggc ttcaccgagc ccgcagcggc ggaaggaaag ggagtccccg ggggccgccg
     1621 cggcgagtag gccgaggtcc gcgcttatgg cggcggctat cttggggtag ccgcccgtgg
     1681 tctgcccgtc ggccatcatg atgatgggca ggccgttgcc ggggacctgc accgcccccc
     1741 acgggacggc gtcggaaatg atgtccgccc cttcccggtg atgaacggcc gggccccgga
     1801 gccgatagcc catccggtcg gaccccgggt ctatacaata ggaagacttg taaaagactt
     1861 cgagcccctc ctcagtaaaa tgaccctcct gcggtcctgg gatgacccta accacggggc
     1921 cttcaataaa acccgggcgg agttctccgg ggaccgcgaa accttccccc cggggcgaca
     1981 ggatgtcggg gacgcccagg gggaggatgt ctcccttttc gaggcgacgc cccttgtacc
     2041 cgcccagctt ccccctcagg taggtggacc tgctccccat gacggggggc gtatcgatgc
     2101 cgccgccgac gcatacccag cacctcgccc ccccgggccc ggcgccaccg aaggtgagga
     2161 cgtcgccctc gcgaagcagc accccctccc agcaggggat ggccttgccg ttgaccctcg
     2221 gtgagaggtc gccgccggtg acgcagacca gcctctcgga ccggaaggcg aggcccgggc
     2281 cgacgagggt cacctcaagc cccgcggccc ccgggccgtt gccgacgacc aggttccccg
     2341 ccgccaggga gaaggggtcg agcgctccgg acaggggcat gccgaaggcc cggaagccgc
     2401 tccgccccag gtcctggacg gtagtcagca aacccggcga ttggacctcc aggctcaagg
     2461 cgtcacgccc cttcctcctc ggacgatctc ctcgaactcc cccgccgtga ctggtacgaa
     2521 ccttatgtcc atccccgtcg ccatgagaaa gggcggctcc cgccccgggt cgaagagggc
     2581 caggggcgtc cttccaatga tgttccaccc tccggggctt tcgatggggt agatgccccc
     2641 ctggcgcccc gctatcccca cgctgcccgc tgccaccagc cccctggggc tctcgagcct
     2701 cggcgtctcg agggcagggt ccaggccgcc caggtagggg aaccccgggg tgaaccccat
     2761 catgaaaacc ctgtagcggc cgccggagtg gcgacggacc acctcgtccg gttcaagccc
     2821 cgtgtgcccg gagaccctct ccaggtcggg gccccaggtg ccgccgtagc agacggggac
     2881 ggaaaaggtc cccgtcggct gggggggctc ccctttcgcc gatagggcca ggcgggccac
     2941 aagggcttcg atctcatcgc gagggccttc ccagggatcg acgtagacgg ccagcgaccg
     3001 gtaggtggac accgtctcca ccagccaggg gggcgcgagg gtcgcgagtc tcaccccgag
     3061 cgccaggacc cgggagttca cccgggggtc gatggcatcg ccgaactcgg ccatcaggca
     3121 ggcgtcgccg caccatgcca ggcggggcgc gtcctccccg gcacgcctag aggccaccgg
     3181 tcagcccctc tttcgacggg ggcgagccgc cgcggcggta acgggccggg ccgtcccggt
     3241 agaggtcgtt cccgcgggcg tcgaccagga ccaccacggg gaagtcctcc accctcatcc
     3301 tgaagaccgc ttcgggcccc atttccggcc aggctacgga ctccaccctg gtgacgcagc
     3361 cggagaggag aaccgccgcg ccgccggcgg ccccaaagta gagggccctg ttcttcacga
     3421 tggaatcgat gacttccggc gaccgccttc ccctgcctat gatccccttg aggccgaggc
     3481 cgaggaggag cggggtgtag gggtccatcc tgccgctcgc gaccggcccg atggggccgc
     3541 agaccctgcc gaggggcgtc ggggagggct ccaggtagca gaggacctgc cccttcaggt
     3601 cgaaggggag gggcctgttc tcacgggcgt attcggtcat ccgtcggtgg gcctcgtcca
     3661 gggccgtgag gatgaccccg ctgatgaggg ccctgtcacc ggtacggagg ggcctcaccg
     3721 cttcgtcgtc taggggagcg accagtctcg aggcgtgcct catcgggcgt acctccctat
     3781 atcgtgactg ccgcgtgccg ggcggcgtgg cagcagatgt tgaccaccac cggcgtcccg
     3841 gcgacatggg cgggggcgaa gtccatgtgg atgtccaggc aggtgacggt cccccccagc
     3901 ccggccggcc ctattccaag ggcgttcacg gcctccaggg tccgcagttc cagctccgcg
     3961 tagcgcctgt cgggattcct tcgccccacg gggtagaggg tggccctctt ggccatgacg
     4021 gcggccgcct ccaccgtccc ccctatgccg acacccacga gggtgggggg gcacggttcg
     4081 gggcccgcgg cttcgacggt gtcccggacg aactcaagta gctcccgggg cccctgatcg
     4141 ggcgacagta cccggagggc gctcacattc tcgccgccga agcccctggg ggcgaccagg
     4201 agccccaccc tgtcgccggg gacgatcctc gtgtggagca ccgccggggt gttcgtgccg
     4261 gtgttccggc ggtcaaagag agggtccgtg acgatggact tccggaagaa cccctcgcga
     4321 taggcttcct ccacgcccga atccaccgcc tcgaccaggc cgcctcccac caggagcacc
     4381 tcctgcccca ggtcgatgaa gaccacggcc atgccggtgt cctggcagag ggggacatgg
     4441 ccgtccctgg cgaggtcgtg gttgcgcagg acctggtcca gcacggacct gcccaggggg
     4501 gactcctcca gcctgtccct cgcgtgggcc agggcgttgt acacgtcctg cccgatggac
     4561 acgttggcct tgaagaaaag acccctgacg gcctcggtga cgagggacgc ttcgacctct
     4621 ctgacctttg ccattgggtt gatcccctct tccagggtgc tgtccttggt ccgatccgta
     4681 aaagactacc tccgcagccc ggtagccctg cccggcatca caggggagcc gaccctcttc
     4741 gcgcgggttt cgaagagcgg acgctcctca tcgggggaag cctgcttctt cggtcgccgg
     4801 gggggcacct ttttcagccc gctcccagtc agggggacca ccaccgtgtc ttcggcggtt
     4861 atcacgcccg ccgccctgag ggccctctcg gcggcaggtg ccgcggccgc cgtgggctcc
     4921 accagcagcc cttcctcgag ggccaggcgc tcctgttcgg ccaggatatc ctggtcgtcg
     4981 acggtgatga catcgcccct gctggcgcgg agggcgtcga tcatctctct catgcgcggg
     5041 ggggccgtta cggcgatccc ctcggcgatg gtggccctga aggtcttctc ttccgtccgg
     5101 ttccactcgg cgtagagggg ggcgcattgg gtgccctggg cggcgtagat cctcggcatg
     5161 cgcctcaccc tggcccgctg gaggagttcc ctgaagccct tgaagacgcc cagcagcatc
     5221 gtcccgttgc cggccgggac caccacggca tcgggcgccc tgaagccgag ttgctcccat
     5281 atctcgaagg ccaccgtctt tgtgccctcc aggaaaagag ggttccagac atggctcgcg
     5341 tagtaagtgc tccccgaaag ggaaaaggcc accgaggccg ccgccgcgcg gtccccgtcg
     5401 acgatctcga gggtggcccc gtaaagttcg atctgccacc tctttgcctc ggcggtgtcc
     5461 ctgggcacga tcaccgttgc cctgatgccg caccgggcag cgtaggccgc tatggacgcc
     5521 ccggcgttcc ccgaggaatc ctgaacgacg ctctcctgac cctggttttt cgcctccgtc
     5581 atgaccagca ccgaaccacg gtccttgaag gaacccgtgg ggaacaggta ttcaagtttg
     5641 taatagggcc caccggagcg ggaacgcacg aggggggtcc agccctcgcc catggaaacg
     5701 gaggggtcca gcggcggcag ggcgctggaa taccgggcca taccccgggc cccggggtcg
     5761 atctccaggt cggcccagtc gacggtgctc ttgagcgggg tcccgcaatc gcaactgacg
     5821 ggcctcgagg tttcattaaa tgaacggccg cagcgggggc aaaagtaaat taccaggaag
     5881 atcacctctc aaagggccgg gtcccgcgac cccggctgct ttttaatata acattacccg
     5941 gggcgggaag gaagaaggcc ggtttgacaa gccggcttcg cagtcgtata aaggaggcgc
     6001 aatgggggag tcggaacggt ccgactgaga ggagacacag gtctcgaccc ctggaacctg
     6061 atccgggtaa tgccggcgaa gggatattgc gggacaattt caaggaagat gccccgaaag
     6121 ccccccggaa cccggggggc ttttttcttg tcccccccca agtcaggctc cctctcaaag
     6181 accctatcga agggagacaa gggaagcgat ggagagcgaa aaggtgagga tcctggtgga
     6241 gggcgccatg acggcggccc tggcggtggc gctatcctac gtgaggatat ggaggatgcc
     6301 ccagggggga tccataacgc tggagaacgt ccccctcttc ctcttcgccc tccggtgggg
     6361 cgtgaaggcc ggcctcggcg cgggatcggt ggcgggtctc ctgcaactgg tgctgggcgg
     6421 ctacatcatc catcccgccc aggccctgct ggactatccc ctggcctttg ccgccctggc
     6481 gttggccgcc ctctggagga agcccctgtg ggcggggttg acgttgggga cctcggcccg
     6541 cctcttatgt cacgtcctct caggggcgat attcttcgcg gcctacgccc ccgaggggag
     6601 caacgtctgg ctctactcca ccgtctacaa cgctaccttc atggtgccga acctggccct
     6661 ttcgatcgtc gccgtctaca tcatctggcc caggctttcg aaaagggcat gaaaaagggc
     6721 gggaagccgg gtcaacccgg cttcccgccc ctatcggcgg cgatgagatc acttgccttt
     6781 caggaggccc accacgaaca gcagcacgac cgccccggca aaggccatga cgagagaacc
     6841 gataaagccc atggggtaga tcccgatgga gctgaagatg acccccccga tgaaggcgcc
     6901 gatgacccct acgcctaggt tgccgaagaa gccgaagccc ctcccctgcc acaccttccc
     6961 ggccagccat cccgcgatga tgccgacgac caggtaaccg acaagatcat tcatggaacc
     7021 cctcaccccc gttccgggca agtcattcta tctaggtgtc ctcctcggcg aggtcctcct
     7081 cgcccactgt ctcctccagg gagagccact tgccccagtc gacgccggcg aagccgctga
     7141 caatatagat gagcatcccc gcgagggggg ccttgttcct caggacggcg atcagcatga
     7201 aggcggaggc ccacatgaag acggccttgc gcttgtttag gctgccggcc ttcagggctt
     7261 tcaggttgcc gaagggaacg ctggagatca tcaggaatcc cgccgccgtg accaggaggg
     7321 ccatgaggac cgggggaagg tggatgcccg ccactacgaa ggagaccagt aaatggccgc
     7381 cggcgggtat gggcaggccc tggaaggagc cgtccacgtg gtggacgttg aacctcgcga
     7441 gcctgagcgc cccgcagagg gcgaaaaagg ccgccacgag ggccccggtg acgccgaaga
     7501 agcccttaag gtaaaccgag tagatgatca tggccggggc gacgccgaag ctcaccacgt
     7561 cggcgaggct gtccagttcc atgccgaagc tgctgctgcc ccccagcttc ctggccatct
     7621 tgccgtccat gaagtcgaag aacacggcga agaagaccag ccacgaggcc gggacgaaat
     7681 gaccgtggaa ggccaggacc agggagagca tgccgctgag catgttcccg caggtgatca
     7741 tgttgggtac cagggtcttc atcggtacgg gcttgcctct acgttctcgg ctcatatcat
     7801 tttctcactc ctatcgctgt cttcccggcc ttgaccttcg ccccgaccgt cacgaccggt
     7861 gaaactccct ccgggaggta caggtcgact tttgagccca attttatcat gccgaaccgc
     7921 tcaccgcgag ccagggcgtc ccccttcttc ctggaacagg ttatcctcct ggccaggaac
     7981 ccggcgacct ggaccgtcat ccccctgcca tggcgggtct ggaaacccat gtagaaccgc
     8041 tcgttctcga gggaggcctt ggggttgtag gccataagtt tcttccccgg cacgtactcc
     8101 atgaaggtca cggtgccgtc gaagggcatc ctgttcacgt gcacgtcgag gggcgacatg
     8161 aagaccccca ccacggtgct tttgccggta aaggggtgct ccgaggggcg gatctccgtc
     8221 accctgccgt cggcggggct cacccaggcc tccgaccctt caggggtggt cctttcaggg
     8281 tccctgaaga accaggcgag aaaaaccgcc gccgccccga gaaggcaccc ggcaagggca
     8341 cccagggtca gggccgcgag gaccgccgcg ccggcgaaga acaggaccgt tcttttccca
     8401 tcgggggcga gcatgggccc ttattccccc tccccctcgc cggaccgtat gacgcttata
     8461 tcggccacca tgtcaccctc ggcgaccttt accaccctgt aaccggtggc ggtcctcccc
     8521 agggtggaga cttcgtccac gttcatgcgg atgacccggc ccctgttggt catgacgatg
     8581 atctggtcct cgtccatgag cccccaggcc cccacgagga gacccgtctt cgaggagatc
     8641 ctcatggccc ggacccccgc ggtggccctg tggtgagccg tgaactcgtc gaaggcggtc
     8701 ctcttgccga cgccgagttc gctcaccagc agcacctggt ggccgggttt gacgatatca
     8761 caacccacta cgctgtcacc cgccttaagg cgtattcccc gcacccccct ggcctggcgg
     8821 cccagcggtc tgaactcgtc ctcaaagacc cgcagggcct gccccttggc ggtgaccatg
     8881 agtatttcgt cctcaccgct ggtgaagcgc accctcgcta tctcgtcgcc gtcgtccagg
     8941 gagagcactc ttctccccgc ccgggtgagg ttctccagct ccccggcggg gagcctcttg
     9001 gccactcccc tggcggtgat gaagaatacg aagctggcgc cccggaggtg cctgtccttc
     9061 atggccacca cggactcgcc gttctcgagg gagatgaact ggctcgcgag gcgtcctttg
     9121 cccgtccggg gctcggggat ggcgtgccct ttcagggcga agaccctccc cttgttggtg
     9181 aaaaggaaaa tgtccctgtg ggtggaggat acggttatga tcgccgcctc gtcccccacc
     9241 ttcggtgtcg cgcccttcac gcccttgccg ccgcgcccct ggagccggta ctgctcgagg
     9301 ggcatcctcc tcaggaaacc atccctggag aggaccacga cgatctcctc ctcggggatc
     9361 aggtcctcga tggagacctc gtccatggcg tcgacgatgg tcgtcctcct gtcatcggcg
     9421 aaatcccggc ggacttcccg cagttcctcc cggacgacca tgtcgaggg
//
LOCUS       DAOSRG010000034         9306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_7119_length_9306_cov_11.184521, whole genome shotgun sequence.
ACCESSION   DAOSRG010000034 DAOSRG010000000
VERSION     DAOSRG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_9306_cov_11.184521"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLM30_02440"
     CDS             <1..962
                     /locus_tag="PLM30_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR54060.1"
                     /translation="MGRPLRIEYPGAFYHVTSRGNEQKDIFKSEGDREKFLTYLESAA
                     ERYGAVFHAYCLMSNHYHLMIETPEGNLSRIMKHINNSYTNYFNVKRKRAGHLLQGRY
                     KAILVEADAYAAELSRYIHLNPVRAKMVALPEEYKWSSYRFYQERTASPWISTGFVLG
                     YFGTDPAKQRIHYRDYILEAIGKKSPDLLSGSIASTILGSDDFVRYIKETYLEGMAPD
                     RDIPALRELQGRPDIGKIKAAAGEAFPDSGRLARVAGVYLCHRFSGAKLQEIGNLFDL
                     TPSGVTQARKRFDEVMKKDKVLEKKVLEVARKLNLPMAQRRTH"
     gene            1074..1931
                     /locus_tag="PLM30_02445"
     CDS             1074..1931
                     /locus_tag="PLM30_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOR54061.1"
                     /translation="MSRSMQKDQENNYRIRFLKSLSEEGLTVFSTSDARRIAEESGIP
                     GSYINKFLSFLTRDGWITRLKRGLYARIGALSGDVQLHSFAIATNLVVPSAISNWSAL
                     HHYGLTGQVPRIITSITPKNVVTPSMRRDSPTGKDFQRHGWLVDGVRFQYKRVSKEHF
                     FGIERIWIDQYSRIPITDRERTVLELFVSPKSFGGMGEVLGIMEEHLFSLEIEKLTGY
                     ALRYGKIAPAKRLGWLLERLSVPERVLDPLLAIPATGYHLLDPAGRQEGVFDRRWNIR
                     VNLAGRKAP"
     gene            2009..2809
                     /locus_tag="PLM30_02450"
     CDS             2009..2809
                     /locus_tag="PLM30_02450"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOR54062.1"
                     /translation="MSYILAGVSSHPILKDTLVFKGGTALKKVFFGEYRFSEDLDFSG
                     MGAPKGDELERCVAEAIELSRSLLLEQGLFSLLLEKYQERDPHPHGQEAFIIRVQFQW
                     HPRPLCRVKMEISHDEPILLAPEKRPLLHGYYEENLRREVTCYCLEEIIAEKLRTLLQ
                     THEKLITRGWNRPRSRDYYDLWQILTKLGYAFKPEKVVEILYLKNEHRNVSFSSPDDF
                     FSQELVSEAYRNWDNSLSAFVMGLPGFDQVLEELKPLVKTLLGEPYPG"
     gene            2858..>3549
                     /locus_tag="PLM30_02455"
     CDS             2858..>3549
                     /locus_tag="PLM30_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR54063.1"
                     /translation="MIYFDNSATTLIKPPEVAKAVKYAIDHFGNAGRSFYGAVMLANR
                     EIYRTRKEIADLAELEDPLRVAFTSSSTESLNLVIRGLVKKEDHVITTVLEHNSVLRP
                     LYLTGCDLSILECDDKGDLLLDTFESLAKPSTKFCVCTHGSNVIGNVTDIGTLYAKCK
                     RHGITLILDVSQTFGAIPVTADMADILCFTGHKALFGPQGTGGIIAKGPIEFAVVKTG
                     GAGVNSFERSQST"
     assembly_gap    3550..3649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3746..>4888
                     /gene="selD"
                     /locus_tag="PLM30_02460"
     CDS             3746..>4888
                     /gene="selD"
                     /locus_tag="PLM30_02460"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOR54064.1"
                     /translation="MKMEYLITFKNTNQAIKAERCLEERGLKVTVLPLPSKIRAGCGI
                     CLKLRAEDIEPALSALTTNEVHDIGVYSMEGAKSDVSFKKVEDLNTLKGKKLHSLLDN
                     CTSGGCGAKISPGELSKLLTSLPVRSDANLLVGYGSSDDAAVYRIDDHRSLVMTADFF
                     PPMVDEPRTFGRIAATNALSDIYAMGGEPLTALNLVCFPESLDLEILNEILTGGAEKV
                     MEAGAVLAGGHSIYDKEPKYGLAVTGIIDNGRIIRNDTPERGHKLILTKPLGTGIIMA
                     ALRGEVASRAAVDKAVAAMERLNKYAAEKMRNYTVSACTDITGFGLMVHMLEMASDKV
                     SILLYSGEIPYFEEAYQYAEEYLVTAAGQRNRNFLAGKADVSGLPFA"
     assembly_gap    4889..4988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4989..5552
                     /gene="yedF"
                     /locus_tag="PLM30_02465"
     CDS             <4989..5552
                     /gene="yedF"
                     /locus_tag="PLM30_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOR54065.1"
                     /translation="KKALGQGDVEAVTVKVDNIVAVQNLEKMARGYGYDFSYQEVSSG
                     LFDVLIVKSGAAAPAVEEERSEQGEVRPSAAPERMVVVIGKDGMGAGSDELGKILVKG
                     FIYSLTELKTPPESILFLNSGVFLTAEGSNAIDDLKRLEAAGTNILSCGTCINYFELQ
                     GKLAVGSVADMYGITEKMARASNTISI"
     gene            complement(5661..6521)
                     /locus_tag="PLM30_02470"
     CDS             complement(5661..6521)
                     /locus_tag="PLM30_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR54066.1"
                     /translation="MELDKGYYKGKTAVVTGAASGIGLALIEELLAYGAEKVVLADFN
                     AVNLKKHTDRLSAEYPGRVKGILCDVTKENEVVDMIAEAAEFFGGRVDLLINNAGAGV
                     MGMFTESYPPIPEKDRHGMIVQTNKDWEKAFALNFYGALYGCREVIPIMQKNGGGQVI
                     NVISGIAFYPMAYQSMYAATKAALNGLTLSLRYEYWDDNIKFNSATPGTTATAIWGDM
                     APPPTAQTPQQSARRILAQAAKNRRVIFGDDPDEAGAKNCFHPDHEDIPDTYLLEVAR
                     ARRSGESTRV"
     gene            complement(6544..7392)
                     /locus_tag="PLM30_02475"
     CDS             complement(6544..7392)
                     /locus_tag="PLM30_02475"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOR54067.1"
                     /translation="MDRGYQIPEEKIEDFMKDGAMNDQYGIQLFFNTDPKRAKELLPP
                     PLVPADPSNPMAYAYIVNIRQPTFSPWYMEGGLGIMARLGDYEGVYFLGLMLSGPGSL
                     MGAFSGREGSGLAKKLCERILVERTGDEGRCLIRRGGIDLIDVKLKIGSYNQKGFRME
                     QEGCSRERPAVIGGGCLNHRYDIEHGGFANLRVINYDSPSRIYSWEPATAEMSLASSV
                     DDRWGELPVTNVIGAGWMVSDNWVTSQKKIYDYTDEQEIRRAVSLLLPGRYDRCTFLK
                     DHQTYE"
     gene            complement(7411..>9306)
                     /locus_tag="PLM30_02480"
     CDS             complement(7411..>9306)
                     /locus_tag="PLM30_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR54068.1"
                     /translation="RLTIPNRVVMPAMGTNLSATGGGVTDDIIAFYEARAKGGVGLII
                     TEVTRIEGGRGISDPCQLAAYRYSDIADLQRLTGAVQKYGTRIFIQLQHPGRMASPLV
                     AGETPVAPSPVADPSSPGNVPRELTEDECTEMVGKFVFAAYVAKSAGADGVELHGAHG
                     YLINEFLSPAMNFRTDRYGGSFENRMRFVEEIIAGIKSGCGKHFPISVRINAEESLPG
                     GIDPEEAARIAAALERAGADAINVSCYNLGCIEPGSYEQGWKKYMAAAVKKAVSIPVI
                     AVSNIKDPSVAEALLEEGVCDFAGVGRGLLADPEWAAKTFSGGDSVIRRCIGCLSCFA
                     EIANLRQVRCAVNPKTGREREYLYPVRDGEGRAVAVIGGGPAGIEAALVLKERGFAPV
                     IFDGGERLGGTLNTADRGYGKDKITKYVDSLVAQVESAGIEVRLGREVTPEDVGGLDP
                     CGVFLACGGEPCIPPVPGTDLDFVCTAEDVLLGRACPEGRIVVVGSGMTGLETAETLA
                     LKGCGVTMVEMLGEVGPGMYYLVVLDVMSRIGPHDPVILTGHRLERIVPGGVELSRLS
                     DGEKVAVQADSVVLAVGVAPRKGVTEPFKAAFPDAVVIGDARCCGRILEATQDARGRA
                     FTFEPQA"
BASE COUNT         1906 a         2643 c         2664 g         1893 t
ORIGIN      
        1 cgatgggcag acccttgagg atcgaatacc cgggagcttt ctatcatgtc acatccaggg
       61 gtaacgagca aaaggatatc ttcaaaagcg aaggcgacag ggagaaattc ctgacctacc
      121 tggagtcggc ggcggagagg tacggggccg tattccacgc ctactgcctc atgagcaatc
      181 actaccacct gatgatcgag acccccgaag gcaacctttc caggatcatg aagcacatca
      241 acaactccta tactaactac ttcaacgtga aacgaaagag ggcgggtcat ctcctgcagg
      301 gccgctacaa ggccatcctt gtcgaggcgg acgcctacgc cgctgaactg tcccgctata
      361 tacaccttaa ccccgtgagg gctaagatgg tggccttgcc ggaagagtac aagtggagca
      421 gttaccggtt ctaccaggag aggacagcgt ccccgtggat ttccaccggt tttgtcctgg
      481 ggtatttcgg aacggaccct gccaagcaaa ggatacatta cagggactac atcctggagg
      541 ccatcggaaa aaagagccct gaccttcttt ccggctcgat cgcctcgacc attctcggca
      601 gcgatgattt cgtcaggtac atcaaggaga cctacctcga ggggatggcg cccgacaggg
      661 atatccccgc cctgcgggag ctgcagggga ggcccgacat tgggaagatc aaggcggcgg
      721 caggagaggc gttccccgac agcggcaggc tggccagggt ggcgggggtc tacctttgcc
      781 atcgtttcag cggggctaag ctgcaggaga tcgggaacct tttcgatctc acgccctcgg
      841 gggtgaccca ggcgaggaag cggttcgacg aggtcatgaa aaaggacaag gtcttggaaa
      901 agaaagtcct tgaagtggct cgtaagctga atctgccaat ggcgcaaagg cgaacacatt
      961 gataaaagta ctatcgtccg gggttgcaca ttaccagaac tcgttataaa atacactgca
     1021 taaataccat tttttagtat tttcgtagtg atttttataa tggagtttcc gttatgagca
     1081 gatcgatgca aaaggaccag gagaataatt ataggataag atttctaaag tcactctctg
     1141 aagaaggcct gactgtcttt tccacttccg atgcaagacg aattgcggaa gagagcggga
     1201 tacccggatc atatataaat aaatttctgt cctttctgac ccgggatgga tggataaccc
     1261 gcctgaagcg cggcctgtat gcccgtatcg gggcgttatc aggagatgtc cagttacact
     1321 ccttcgccat tgccacaaat ttggtagtac cttccgcgat aagcaattgg tcagccctac
     1381 accattatgg gttaacaggg caagtcccga gaattatcac ttcaataacc ccaaagaatg
     1441 tcgtcacacc gagcatgcgg cgggatagtc ccacgggaaa ggatttccag cgtcatggat
     1501 ggttagtcga tggtgttcga tttcaataca aaagggttag taaagaacat tttttcggta
     1561 tagaaaggat ttggatcgac cagtactcca gaatacctat tactgatcgt gaacggacgg
     1621 tgctggagct tttcgtatcg ccaaaaagtt ttggaggaat gggcgaggtg cttggaataa
     1681 tggaggaaca ccttttttcg ctggaaattg aaaaacttac cggttacgcc ctcagatacg
     1741 gaaagatagc cccggccaag agattggggt ggctgcttga gcgtctttct gtccccgaga
     1801 gagtccttga ccccctcctg gcgatacctg ccacgggata tcatttgctg gaccccgcag
     1861 ggcgacagga aggagttttc gataggcgat ggaatatccg agtgaacctt gcggggcgca
     1921 aggccccatg aaacccctgc gagtccgggt tcgacagact tcccaagcat tggggatccc
     1981 gcaagaagtg gtcgaaaagg actatgcctt gagttacatt cttgcagggg tatcaagcca
     2041 cccgattctg aaggatacgc tggtcttcaa aggtgggaca gcccttaaaa aggtgttttt
     2101 cggggaatac aggttttccg aggatctgga tttttcgggt atgggcgcac caaaaggcga
     2161 tgagttggag aggtgcgtag cagaggcaat cgaactttca cgaagcctcc tcctggaaca
     2221 gggtcttttt tctcttcttc tggaaaaata tcaggagcgt gatcctcatc cccatggcca
     2281 ggaggcgttc atcatcaggg ttcagtttca gtggcatccc cggcctttat gtcgtgtaaa
     2341 aatggagatc agccatgatg agcccattct tcttgcaccg gaaaagcgtc ctcttttgca
     2401 cggatattat gaagagaacc ttcggagaga agtgacctgt tattgccttg aggagatcat
     2461 cgctgaaaaa ctgagaacct tactgcaaac acatgaaaag ctgatcacca ggggatggaa
     2521 ccgtccccgc tccagggatt actacgactt gtggcagatc cttacgaaac tagggtatgc
     2581 tttcaaaccc gaaaaagtgg tggagatact gtatctcaag aatgagcata gaaacgtgtc
     2641 ctttagtagc cccgatgatt tttttagcca agagcttgtt tccgaggcgt atcgaaactg
     2701 ggataatagc cttagtgctt tcgtcatggg ccttcctgga tttgaccagg tacttgagga
     2761 gttaaagccc ctggttaaaa cgcttctcgg tgagccttat cccggttagg atctgtcaat
     2821 tgtgtaggtc tgacacctag gaggaagggt aaagaggatg atctatttcg acaacagcgc
     2881 gacaacgctg atcaagccgc ccgaggtggc caaggccgta aagtacgcca tcgatcattt
     2941 cggcaacgcc gggcgctcct tttacggcgc ggtcatgctg gccaacaggg aaatataccg
     3001 gacgcgcaag gagatcgccg acctcgcgga gctcgaggac ccgctccggg tggcattcac
     3061 atcgtcatcg acggagagcc tcaacctcgt gatccgcgga ctggtcaaaa aagaggacca
     3121 cgtgatcaca acggtcctgg agcataattc cgtgttacgc cccttatatt tgaccggatg
     3181 tgatcttagc attttggagt gcgacgataa gggcgacctg ttgctggata cctttgagag
     3241 cctggcgaag ccttccacca agttttgtgt ctgcacccac ggctcaaatg tcatagggaa
     3301 cgtcaccgat atcgggacgc tttacgccaa gtgcaagcgc cacggcatca ccctgatcct
     3361 tgacgtgtcg cagactttcg gggcgatccc cgtcacggct gatatggccg atatcctttg
     3421 ctttaccggg cacaaagcgc tcttcggtcc ccaggggacg ggtggtatca tcgcgaaggg
     3481 gccgatcgag tttgccgtcg tgaagaccgg gggagccggc gtgaacagct tcgagcgctc
     3541 ccagtccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcatggtccg
     3661 cttttccttt tcctacttca atacgcggga cgagatccgg cggggcgtcg aggcgatcca
     3721 aagtatcgtc cagtccttgg ggtagatgaa gatggaatac ttaattacct tcaaaaacac
     3781 caaccaggcc ataaaggcag aaaggtgcct ggaggagcgg ggccttaagg tgacggtgct
     3841 cccgctgccg tcgaagatcc gcgctggctg tgggatatgc ctgaaactcc gggcggaaga
     3901 catcgagccc gcgctttcgg cgctgacgac caatgaagtc catgatatag gcgtatattc
     3961 aatggaaggg gcgaaaagcg acgtctcctt caagaaggtg gaagatctga acacactgaa
     4021 ggggaaaaag ctccattcgc ttttagataa ctgcaccagc ggcggctgcg gtgcgaagat
     4081 cagccccgga gagctgtcca agctgctgac aagtctgccc gtgcggagtg acgccaacct
     4141 gcttgtcggc tacggttcct ccgacgacgc cgcggtctac aggatcgacg accaccgctc
     4201 cctggtgatg acggcggatt tcttcccgcc catggtggat gagccgcgca cctttggcag
     4261 gatagcggcg acgaacgctc ttagtgacat ctacgccatg ggaggcgaac ctttaacggc
     4321 cctgaacctg gtttgtttcc ctgagtccct ggacctggag atcctgaacg agatactgac
     4381 gggcggcgcg gaaaaagtca tggaggcggg tgccgtcctg gcgggcgggc attctatata
     4441 cgacaaggag ccgaagtacg gcctggccgt gacggggatc atcgataacg gccgtattat
     4501 caggaacgac acgccggaac ggggtcataa actcatactg acgaagcccc tcggcaccgg
     4561 gatcatcatg gcggccctgc ggggtgaggt cgccagccgg gcggcggtcg acaaggctgt
     4621 ggccgcgatg gagcgcctga acaaatacgc ggcggaaaag atgaggaact atacggtcag
     4681 cgcctgcacc gatataaccg gtttcggcct gatggtgcac atgctggaga tggcttcgga
     4741 caaagtatcc atcctgctat actcgggtga gatcccctat ttcgaagagg cctaccagta
     4801 cgccgaggag tacctggtca ccgccgcggg ccagcggaac cgcaacttcc tggcgggcaa
     4861 ggccgacgtc tccggtttgc ccttcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnaa aaaagcgctg gggcaagggg acgtggaggc cgtcaccgtg aaagtggaca
     5041 acatcgtggc ggtgcagaat cttgagaaaa tggccagggg ctacgggtac gatttttctt
     5101 accaggaggt ttcgtcgggc cttttcgatg tccttatcgt taaaagcggc gccgcggccc
     5161 ccgctgttga agaggagagg agcgaacaag gcgaggtccg cccttccgcg gctcccgaaa
     5221 gaatggtcgt cgtgatcggc aaggacggca tgggggctgg ttccgacgaa ctgggcaaga
     5281 tcctggtcaa gggattcatt tactccctga cggagctcaa gacacctccc gaatcgatat
     5341 tattcttaaa ctcaggggtg ttcttgacgg ccgaaggctc caatgcgatc gatgacctga
     5401 aaagactgga agccgccggg acgaatatcc tgtcctgcgg gacctgcatc aattacttcg
     5461 agctgcaggg caaactggcc gtcggcagcg tagccgacat gtatggaata acggaaaaga
     5521 tggccagggc gagtaatacc atcagcatct gaccgcacgg ggagatcttc tcccagcccg
     5581 gacagcaccc tgcaaagagg gtgggcggcc cctggggctc gcccaccctg tccgcgttgc
     5641 taggaaaccg ggccgtgcct ctagacccgg gtggactcgc cgctccttct ggcccttgcc
     5701 acctccagaa ggtaggtgtc cggtatatcc tcgtgatccg ggtggaagca gtttttcgcc
     5761 cccgcctcgt ccgggtcgtc gccgaatatg acccgcctgt ttttcgcggc ctgggccagg
     5821 atgcgccttg ccgactgctg gggggtctgg gcggtcggcg gcggggccat atcgccccag
     5881 atagccgtgg ccgtcgtgcc gggcgtggcg gagttgaact tgatgttatc gtcccagtac
     5941 tcgtagcgca gggaaagggt caggccgttc agggccgcct tggtggccgc gtacatcgac
     6001 tggtaggcca tggggtagaa ggctatgccc gagatcacgt tgatcacttg cccgcctccg
     6061 ttcttctgca ttatgggaat aacctcgcgg caaccgtaca gcgccccgta gaagttgagg
     6121 gcgaaggcct tttcccagtc cttgttggtc tggacgatca tcccgtggcg atccttctcg
     6181 ggtatggggg ggtacgactc ggtgaacatc cccataactc ccgccccggc gttgttgatc
     6241 agcaggtcga ccctcccgcc gaagaactcc gcggcctcgg cgatcatgtc cacgacctcg
     6301 ttctccttcg tcacgtcgca gaggatgccc ttaactctcc cgggatattc ggccgaaaga
     6361 cggtcggtat gctttttcag gttcacagcg ttaaagtccg cgagcacgac cttctccgcc
     6421 ccgtaggcca gcagctcctc aatgagggcc agccctatgc ccgacgccgc cccggtgacg
     6481 acggcggtct tgcctttgta gtagccttta tctaattcca tcggtgcatc ccgcctttct
     6541 tgtctattcg taggtctggt ggtcctttag gaacgtgcaa cgatcgtagc ggcccggaag
     6601 cagaagagac acggcccgcc ggatttcctg ctcgtccgtg tagtcgtaga tcttcttctg
     6661 acttgtgacc cagttgtcgc tgaccatcca gcccgctccg ataacgttgg tcacaggcag
     6721 ctccccccac ctgtcgtcta cggaggaggc cagcgacatc tccgccgttg caggttccca
     6781 tgagtagatg cggctggggc tgtcgtagtt gatcaccctg agattcgcga agcctccgtg
     6841 ctctatgtcg tacctgtggt tgaggcaacc gccgccgata actgccggtc tctcccttga
     6901 gcacccctcc tgctccatcc tgaagccttt ctggttgtag gatcctatct taagtttcac
     6961 gtctatcaga tcgatcccgc cgcgacgtat gaggcatctt ccctcgtcgc ccgtcctctc
     7021 gacgaggatc ctttcgcaca gcttcttggc aagcccggac ccctcgcgcc cggagaaggc
     7081 tcccatcagt gagccggggc cgctcagcat cagccccagg aagtagacgc cctcgtaatc
     7141 tcccaggcgc gccattatcc cgagcccacc ctccatgtac catggggaga aggtgggctg
     7201 gcggatgttc acgatgtagg cgtatgccat cgggtttgaa gggtccgccg gcaccagggg
     7261 cggcggcagc agttccttcg cccttttcgg gtcggtgttg aagaaaagct ggatgccgta
     7321 ctggtcgttc atcgcgccat ccttcatgaa gtcctcgatt ttttcctccg gaatctggta
     7381 gcctctgtcc ataataaact ctcctttcgt ctatgcttgc ggttcaaacg tgaaggccct
     7441 tccgcgagcg tcctgcgtgg cctcgagtat gcgcccgcag cacctagcgt cgcctatgac
     7501 cacggcgtcg ggaaaggcgg ccttgaaggg ctccgtcacg cccttgcgcg gggcgacacc
     7561 caccgccagc acaacgctgt ccgcctgtac cgctaccttc tctccgtccg acagccgcga
     7621 aagctccacg ccgccgggga cgatccgctc cagcctgtga ccggtcagta tcacgggatc
     7681 gtggggcccg atgcggctca tcacgtccaa caccacgagg tagtacatgc ccgggccgac
     7741 ctcgccgagc atctccacca tggtgacccc gcaccccttg agggccagcg tctcagccgt
     7801 ctcaagcccg gtcatgcccg atcctactac tacgatcctc ccctccggac aggcacgccc
     7861 cagcaacacg tcctccgcgg tgcagacgaa gtcaagatcc gttccgggga ccggcgggat
     7921 gcagggttcc ccgccgcacg ccaggaagac gccgcagggg tccaggcccc cgacgtcctc
     7981 gggggtgacc tcccgcccca gccggacctc tatcccggca ctctcgacct gagcgaccag
     8041 agagtcgacg tattttgtga tcttgtcctt gccgtaaccc ctgtcggcgg tgttcagggt
     8101 cccgcccagc cgctccccgc cgtcgaatat gaccggggca aatccgcgct ccttgagaac
     8161 aagggcggcc tcgatacccg cggggccacc gccgatcacg gcaacggcgc gcccctcgcc
     8221 gtcgcggacc gggtacagat actcgcgctc gcgaccggtc ttcgggttca ccgcgcaccg
     8281 cacctgcctg aggttggcga tctcagcgaa gcagctgagg cagcctatac accttcgaat
     8341 gacgctatca ccgccggaga aggtcttggc ggcccattcc ggatccgcca gcaggccgcg
     8401 gccgacccct gcgaagtcgc agaccccctc ttcgagcagg gcttcggcaa cggacgggtc
     8461 cttgatgttg ctcacggcta tcacggggat ggagacggcc ttcttgacgg ccgccgccat
     8521 gtacttcttc cacccctgct cgtaagaccc gggttcgatg cagccgaggt tgtagcagct
     8581 cacgttgatc gcgtccgcac cggctctctc caaagcggcg gcgatccggg cggcctcttc
     8641 ggggtcgatc ccccccggga ggctctcctc ggcgttgatg cgcaccgaga tggggaagtg
     8701 cttcccgcag ccgctcttta tcccggcgat tatctcctcg acgaacctca tgcgattctc
     8761 gaaggacccc ccgtaccggt cggtgcggaa gttcatggcg ggggagagga actcgttgat
     8821 caggtagccg tgcgcgccgt gaagctccac tccgtccgcg cccgcggact tcgccacgta
     8881 ggcggcgaag acgaacttgc cgaccatctc ggtgcactcg tcctcggtca actcacgcgg
     8941 cacgttgccc gggctcgatg ggtcggccac cggggaaggg gccaccgggg tttcgcccgc
     9001 caccaagggg gaggccatgc ggccggggtg ctgaagctgg atgaagatcc tcgtgccgta
     9061 cttctgaact gcgccggtca gccgctggag gtcagcgata tcggagtacc ggtaagctgc
     9121 gagctggcac gggtcgctga tgccccggcc cccttcgatt ctggtcacct cggtgatgat
     9181 gagaccgacg ccgccctttg ccctcgcctc gtagaaggcg atgatgtcat cggtcacgcc
     9241 gcctcccgtg gccgacaggt ttgtccccat cgcgggcatt accacgcggt tggggatggt
     9301 caagcg
//
LOCUS       DAOSRG010000035         8839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_7551_length_8839_cov_9.136157, whole genome shotgun sequence.
ACCESSION   DAOSRG010000035 DAOSRG010000000
VERSION     DAOSRG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7551_length_8839_cov_9.136157"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLM30_02485"
     CDS             <1..1037
                     /locus_tag="PLM30_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOR54069.1"
                     /translation="IMGKTIIIGKKDNLSRTMGMEKTRSFRISYAEPSDIKGLLNDLF
                     GVTNVVVDDRLRTIYVTASEERLEDVAVTLQRIDHPGRQVMLQARIVEVSDTGSDEVK
                     TLVNAVYERWYASFSSSGGTLGYYDDVDKTGIYDPARTGREVNPPESITFPDMAGSAL
                     GFFDAGLQLLVKDEKAKMLASPSVVTIDGQKASIKLLTDIKYVSERDDQGRPTYGEVE
                     SGPALEFTPTVGRSDIVTIEMNVKTGDAKLTYDSLLRGYIPESSTREVQTVVRVRDGE
                     PFVVGGLFHESKTHNVWKIPVISEIPLLGELFKGRSTNVSKSEVIMVVVPYILDVPET
                     LIQGSDVRLN"
     gene            1161..1733
                     /gene="efp"
                     /locus_tag="PLM30_02490"
     CDS             1161..1733
                     /gene="efp"
                     /locus_tag="PLM30_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR54070.1"
                     /translation="MGQVVDTSSFHSGMKVLWQGGIWEIIDCQHHKMGRGGAIVRTRL
                     RNLDNGSIVENSFRSGEKLERVIFDDKAAQFLYQDGENYVFMDMENYDQIYIHKDSLG
                     SAAGFLTDNLEVGLEVYEGKIMGVELPKSVELKVVETSPGYKGDTVSGSGKPATLETG
                     LTLTVPMFIETGEVIVVDTRTGEYLERAKR"
     gene            1750..2142
                     /locus_tag="PLM30_02495"
     CDS             1750..2142
                     /locus_tag="PLM30_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54071.1"
                     /translation="MKMGAREKIAFLEGLLEGLDPKGDPERKIYFAVVEALHALADEI
                     DDHEEILEEHRETIEDLSEYCEQLEEDMERLEGDLDDVDDLLEDEPGETFDSLECPEC
                     GHVFFFKPEARAEDGALQCPECGFVIQD"
     gene            2178..>2545
                     /locus_tag="PLM30_02500"
     CDS             2178..>2545
                     /locus_tag="PLM30_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOR54072.1"
                     /translation="MSPSKKELDQVEPVMGVDAPATDPLMPEDKSEGTIKISEEVIGQ
                     IATQALLKVNGVQPASPGLVANLRLGKKTSGGVRISVDEGETPSVMVDAFITTKYGLR
                     IPDVAWDVQESIKKTLEQFTG"
     assembly_gap    2546..2645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2731..3237
                     /locus_tag="PLM30_02505"
     CDS             2731..3237
                     /locus_tag="PLM30_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54073.1"
                     /translation="MFYFWRSGEVGELWLSGEGLRDAFSRMHPEGPECSEVILLADRD
                     TLNASFVREPGLTPLAMATAEADFREFALELGFSDIQAGWTRQGGLGESLAASSLARN
                     PLAWGAAAWVLAAVILMGLSGALLATAWGVAAFAVAALFTTERGDEIRRWFQKRLGKR
                     SGGGTGVS"
     gene            3234..3701
                     /gene="nusB"
                     /locus_tag="PLM30_02510"
     CDS             3234..3701
                     /gene="nusB"
                     /locus_tag="PLM30_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR54074.1"
                     /translation="MKAILPQKRRRSREIALQILYSMEMREGVTPELAVELYPFDAED
                     EEVKSYAALLVLGVFKSKNGIDDLVRKHIVGWRPERMVIVDRVAIRLALYESVVAGMI
                     PVPVAISEAVELSKQFGTAESGKFVNGVLGKIVRALESEEGAESADGGDPDRR"
     gene            3676..4902
                     /gene="xseA"
                     /locus_tag="PLM30_02515"
     CDS             3676..4902
                     /gene="xseA"
                     /locus_tag="PLM30_02515"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOR54075.1"
                     /translation="MAGILTVDELTRYITFLFEESEVLSHLQVKGTLSGFKRHTSGHV
                     YFTLLGDQTRVSCALFRSDAGRVPSWPADGDEVLVTGKAGVYGPRGTYQVYARTIRPL
                     GEAAQTRAKKELLERLQKEGLFEPSVKRRLPPLPLEVAVVTSPTGAAVRDVIKVARER
                     FPLCRIIVVPTLVQGAGAIEEITRGLSRAGNLPGVEAVMLVRGGGSRDDLNPFDEEEV
                     VRAVRNCRVPVITGLGHQVDRTLSDLAADYYAPTPSAAAEALFPDRGDLLRRVAGSKA
                     LMAASLDREMHRHRLLLDGRVDSLARSFERSALFPAGTELEKLRVRAVTSIERIAALS
                     WSLFGEAASALDALSPLKTLARGFASCSKADGSPVARAAEVSSGDELVVAFLDGSATT
                     RVDSVRGSGLRPGARA"
     gene            4899..5918
                     /gene="mtnA"
                     /locus_tag="PLM30_02520"
     CDS             4899..5918
                     /gene="mtnA"
                     /locus_tag="PLM30_02520"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOR54076.1"
                     /translation="MSVRAVEWDPGDGRLVLLDQRLLPSKVERIRCGDPECVARAIED
                     LAVRGAPAIGIAAAFGMVLAAGGGLEGVKAAAPRLASARPTAVNLPWAVERMLEKALS
                     TPGGKELQPVLLEEARLILEEDRAANRAMGFLGQALLPPKCSVLSHCNAGALATGGYG
                     TALGVVRAARENGKEVKVYLGETRPVLQGARLSAWELAEEGFDVTVICDSMAAALMKN
                     RGVDAVIVGADRVAANGDFANKIGTYGLAILAREHGVPFYAAAPWSTFDTSLPTGEGI
                     VIEERPDEEVRLLPDGGRLPERVKVWNPAFDVTPARYVKAFICEKGVFEPPYDFSALN
                     DKKTK"
     gene            5933..7177
                     /locus_tag="PLM30_02525"
     CDS             5933..7177
                     /locus_tag="PLM30_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOR54077.1"
                     /translation="MKGSRIADASLFREGERKVLWARGRMPVLAKFVERGKNERPLAG
                     VTVGACLHLEAKTACLLLALKELGAEVAAAGSNPLSTQDDVCAFLVREGVAVFSWRGM
                     SGGEYFENLESVLDRSPEILVDDGADLVVTAHEKYPALLPRVKGGSEETTTGVRRLKA
                     MMDQGVLAFPMISVNDARSKFLFDNRYGTGQSVFDGLMRATNVLIAGKVFVVAGYGWC
                     GRGVAMRAKGFGARVVVVETDPHRAFEALMDGCQVCDMMEAASMGDLFLTLTGNRDVI
                     REEHFRVMKDGAILGNAGHFDVEIDKAALERLSKEVFEARDNVTTYVLGDGRRISLLA
                     QGRLVNLAAGDGHPIEIMDLSFGLQLGSVLHLAENRLSPGVYDVPGEIDADVVRTALK
                     ARGISLEVPTREQEEYLRQWRE"
     gene            7184..8479
                     /locus_tag="PLM30_02530"
     CDS             7184..8479
                     /locus_tag="PLM30_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR54078.1"
                     /translation="MAVLFRDFYYIDSKMEGGARGDLLVEGGRIARLGKDLPGPSGGE
                     TIRGEGKALLAPGFVNGHTHAAMVLLRGLGEELPVMEWLQERIWPVEAGLLPEHIYWG
                     TRGAVMEMASTGTTCFCDMYFEMDDVARAASERGMRCCLSRGLTGDDPAKVREGAELF
                     RRWHGRDHVTVQLGPHAPYTVSLEAMKGITALAEEIGAGVHFHFLEAEWEPSFIRDKY
                     GLSPLAYLEEAGLLGSCPAILAHCVWFPPFEIPGLAGTAATVAHNPSSNMKLGSGHAP
                     AQAFLDGGVPLSLGTDGAASNNRLDVWEEMRLCALLHKGALKDPTVMASRDVFRMATL
                     SGAASAGFSDVGLLREGWQADLVMIDLDRPHYIGWDRENIVDFLVYAGFSSDILGTMC
                     AGKWLYRDGEFPGQDYDAVMGEVTRCREDLLRRAARRQP"
     gene            8515..>8839
                     /locus_tag="PLM30_02535"
     CDS             8515..>8839
                     /locus_tag="PLM30_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOR54079.1"
                     /translation="MRWRSGKELREMFLSYFERNGHRRFPSFSLVPDDPTLLFTIAGM
                     VPFKPYFLGKREPSFKRATTSQKCVRTNDIENVGRTARHHTLFEMLGNFSFGDYFKSG
                     AAAFAW"
BASE COUNT         1615 a         2703 c         2960 g         1461 t
ORIGIN      
        1 ccatcatggg caagaccatc atcatcggca agaaggacaa cctctcccgc acgatgggga
       61 tggagaagac ccgctccttc cggatttctt acgctgagcc gtccgatatt aaagggctgc
      121 ttaatgacct tttcggtgtg accaacgtcg tcgtggacga tcggctccgg acgatctacg
      181 tgaccgcctc cgaggagagg cttgaggacg tggccgtcac cctccagagg atcgaccacc
      241 cggggaggca ggtcatgctc caggccagga tcgtggaggt ctccgatacg ggctccgacg
      301 aagtgaagac cctggtgaac gccgtctacg agaggtggta cgcctccttc tccagttccg
      361 gcgggaccct gggctactac gatgacgtcg acaagaccgg catctacgac cctgcccgga
      421 ccggccgtga ggtcaacccc ccggagagca tcaccttccc cgacatggcc ggcagcgccc
      481 tggggttctt cgacgccggg ctgcagctgc tggtgaagga tgaaaaggcc aagatgctcg
      541 ccagcccgtc ggtggtcacc atcgacggcc agaaggcctc gataaaactc ctcaccgata
      601 tcaagtacgt ctccgagcgg gacgaccagg gcaggcccac ctacggcgag gtcgagtcgg
      661 gcccagccct cgagttcacc cccaccgtgg ggcgttccga tatagtcacc attgagatga
      721 acgtaaagac cggcgacgcc aagctcacct acgactcgct gctgaggggg tacataccgg
      781 agtcgagcac ccgcgaggtc cagaccgtcg tcagggtcag ggacggcgag cccttcgtcg
      841 tgggcggtct tttccacgag agcaagaccc acaacgtatg gaagataccg gtgatctccg
      901 agatccccct gctgggcgaa ctcttcaagg ggaggagcac caacgtctcc aagtcggagg
      961 tcatcatggt ggtggtcccc tacatcctgg acgtccccga gaccctgatc cagggtagcg
     1021 atgtcaggct gaactagact gaaagaggcg ggggactgga aacccggtcc cccaggcgtt
     1081 acactaggcg gggcggcgcg gcgcggcctg cgccgcccaa gggttctatt ctttttttcg
     1141 tcggcaggag ggtccattta atgggtcagg tggtagacac aagcagcttt cattccggca
     1201 tgaaggtcct ctggcagggg ggcatctggg agatcataga ctgccagcat cacaagatgg
     1261 gcaggggagg ggccatagtc aggaccaggc tccgcaacct cgacaacggc tccatcgtgg
     1321 agaactcctt ccggtccggt gagaaactgg aaagggtcat cttcgacgac aaggcggccc
     1381 agttcctcta ccaggacggg gagaactacg tctttatgga catggagaac tacgaccaga
     1441 tctatattca caaggacagc ctcggttcgg cggccggctt cctgacggac aatctcgaag
     1501 tgggcctgga ggtctacgag gggaagatca tgggggtgga actccccaag agcgtggaac
     1561 tgaaggtcgt ggagacctcc cccgggtaca agggggacac cgtctcgggc agcggcaagc
     1621 ccgcgacgct ggagacgggg ctcaccctga cggtccccat gttcatcgag acgggcgagg
     1681 tcatcgtggt ggatacccgg acgggcgaat acctcgaaag ggccaaacgt taaaaccgct
     1741 agggggttga tgaagatggg tgcaagggaa aagatcgcgt tcctggaagg cctcctggag
     1801 gggcttgacc ccaagggtga ccccgagaga aagatctact tcgccgtcgt cgaggcgctc
     1861 cacgccctgg ccgacgagat agacgaccac gaggagatcc tggaggagca ccgggagacc
     1921 atcgaggacc tctcggagta ctgcgagcag cttgaagagg atatggaacg gctcgagggg
     1981 gacctggacg acgtggacga cctgctcgag gacgaacccg gggagacctt cgattccctg
     2041 gagtgccccg agtgcggcca cgtcttcttc ttcaagcccg aggcgcgggc ggaggacggg
     2101 gccctccagt gccccgagtg cggtttcgtg atccaggatt gatactgctt caagatcaac
     2161 gggaggaggg gaaggccatg agcccatcca aaaaagaact tgaccaggta gagcccgtca
     2221 tgggcgtcga cgctccggcg acggatccct tgatgccgga ggacaagagc gaggggacca
     2281 tcaagatatc ggaagaggtc ataggccaga tcgccaccca ggcccttctg aaggtgaacg
     2341 gcgttcaacc ggccagtccc ggcctggtgg ccaacctgag actggggaag aagacctccg
     2401 gcggagtgcg catctctgtg gatgaggggg agacgccttc cgtcatggtc gatgccttca
     2461 tcaccacgaa gtacggcctc aggatccccg atgtcgcctg ggatgtgcag gagtccatca
     2521 agaagaccct ggaacagttc accggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnncaagc cctgatcgaa ccggccgagc ccggttatca agcggccggt ccccctggga
     2701 ccggcctttc atgttatggg aggggtcgga gtgttttact tctggagatc gggagaagtg
     2761 ggtgaactct ggctttccgg ggaaggactc cgggacgcct tcagcaggat gcaccccgag
     2821 ggtcccgaat gctcggaggt gatcctcctg gcggaccggg atacgctcaa cgcctccttc
     2881 gtccgggaac cgggtttgac gcccctggcg atggcgaccg ccgaggccga tttcagggaa
     2941 ttcgccctgg aactgggttt ttcggacatt caggcggggt ggacccggca gggtggcctc
     3001 ggggagagcc tggcggcctc gagcctggcg cgaaatcccc tggcctgggg agccgcggcc
     3061 tgggtgctgg cggcggtcat cctgatggga ttatcgggag cccttttggc tacggcctgg
     3121 ggagttgcgg ccttcgccgt ggcggcgctc ttcaccaccg agcggggaga cgagatacgc
     3181 cggtggttcc agaagcgcct gggcaagagg tcaggtgggg ggacgggggt aagttgaagg
     3241 ccattcttcc ccagaagaga agaaggtcca gggagatcgc cctccagatc ctctattcca
     3301 tggagatgcg cgagggcgtg accccggagc tggcggtgga gctgtacccc ttcgacgccg
     3361 aggacgaaga ggtcaagagc tatgccgccc tgctggtcct gggggtcttc aagagcaaga
     3421 acggcatcga cgacctggtg aggaagcata tcgtcggctg gagacccgag cggatggtca
     3481 tcgtcgaccg ggtggccata aggcttgccc tttatgagag cgtcgtcgcc gggatgatac
     3541 cggtgccggt ggccatctcc gaggcggtgg aactctctaa gcagttcggc accgccgagt
     3601 cgggcaagtt cgtgaacggc gtgctgggca agatcgtaag ggccctcgag agtgaggaag
     3661 gagcggaaag cgctgatggc ggggatcctg accgtcgatg aactgacccg ctacatcacc
     3721 ttcctcttcg aggagagcga agtcctgtct cacctccagg tgaagggcac cctgtcgggt
     3781 ttcaagcgcc acaccagcgg acatgtctac ttcaccctcc tcggcgacca gacgagggta
     3841 tcctgcgccc tcttccggtc cgacgccggt cgtgtgccct cgtggcccgc cgacggcgac
     3901 gaggtcctgg tcacggggaa ggccggcgtt tacggcccga ggggtaccta ccaggtctac
     3961 gcccggacca tcaggcccct gggagaggcc gcccagacca gggcgaaaaa ggaactcctg
     4021 gagcgccttc agaaggaggg cctttttgaa ccctccgtca agaggaggct cccgcccctc
     4081 cccctcgagg tcgccgtcgt gacctcgccc accggggctg ccgtcaggga tgtcatcaag
     4141 gtggcccggg agagattccc cctctgccgg ataatagtcg tcccgacgct ggtccagggg
     4201 gccggcgcca tcgaggagat aacccggggc ctttcccggg ccgggaacct ccccggcgtc
     4261 gaggcggtca tgctcgtgag aggcggcggg agccgcgacg acctcaaccc cttcgacgag
     4321 gaagaggtgg tcagggccgt caggaattgc cgggtcccgg ttataacggg gctggggcac
     4381 caggtggacc gtaccctctc ggacctcgcc gcggactact acgcccccac cccctcggcg
     4441 gccgccgagg cccttttccc cgacaggggc gacctcctcc gccgggtggc cggctccaag
     4501 gccctgatgg cggccagcct cgaccgggag atgcatcgcc accgcctgct cctcgacggc
     4561 agggtggatt ccctggcgag gtccttcgag aggtccgccc tctttcccgc agggacggaa
     4621 ctcgaaaagc tccgggtccg tgccgtgacc tccatcgaga ggatcgctgc cctatcgtgg
     4681 tccctcttcg gcgaggccgc ttcggccctt gacgctcttt cccccctcaa gacccttgcc
     4741 aggggtttcg cctcctgctc gaaggccgac ggttcccccg tcgccagggc tgcggaagtc
     4801 tcctccggcg acgaactggt cgtggccttc ctcgacggta gtgctacgac gagggtagat
     4861 tccgtccggg gcagcggcct caggccgggg gccagggcat gagcgtccgg gccgtggagt
     4921 gggaccccgg ggacgggagg ttggtgctgc tggaccagag gcttttgccc tcgaaggtcg
     4981 agcggatccg atgcggcgac cccgagtgcg tcgcccgtgc catcgaggac ctcgccgtca
     5041 ggggggctcc ggcgataggc atcgccgccg ccttcggcat ggtccttgcc gccggcggag
     5101 gcctggaagg ggtgaaagcc gccgccccga ggctcgcctc ggctaggccc acggcggtga
     5161 acctcccctg ggccgtggaa aggatgctgg aaaaggccct ctccacccct ggggggaagg
     5221 agttgcagcc ggtgctgctg gaagaggcta ggttgatact tgaagaggac cgggccgcca
     5281 accgcgccat gggtttcctt ggccaggccc tccttccgcc caagtgcagc gtcctgagcc
     5341 actgcaacgc cggagccctc gccaccggcg gttacggcac ggccctcggg gtcgtcaggg
     5401 ccgccaggga gaacggcaag gaggtcaagg tctacctcgg cgagacgagg cccgtcctcc
     5461 agggggcccg tctttccgcg tgggaattgg ctgaagaagg cttcgacgtg accgtcatct
     5521 gcgactccat ggcggcggcg ctcatgaaaa accgcggcgt cgacgccgtc atcgtcgggg
     5581 ccgaccgggt agccgccaac ggcgacttcg ccaacaagat cggcacctac ggtctggcga
     5641 tcctcgcccg ggagcacggt gtccccttct acgcggcggc tccctggagc accttcgaca
     5701 cctccctccc tacgggcgag ggcatcgtca tcgaggagcg ccccgacgaa gaggtgcgcc
     5761 tcctccccga cggcgggagg ctgcccgaga gggtgaaggt ctggaacccg gccttcgacg
     5821 tgacccccgc caggtacgtg aaggccttca tctgtgaaaa aggggttttc gaacccccct
     5881 acgatttttc cgccctgaac gataagaaga cgaaatgact ggagatggtc ctttgaaggg
     5941 ttcccgtatc gccgacgcct cccttttcag ggagggtgaa aggaaggtcc tctgggccag
     6001 ggggaggatg cccgtcctgg cgaagttcgt cgagaggggc aaaaacgaaa ggcccctcgc
     6061 cggcgtgacg gtgggggcct gcctgcacct ggaggccaag acggcctgcc tcctgctcgc
     6121 cctgaaggaa ctgggcgccg aggtggccgc cgcggggagc aaccccttat ccacccagga
     6181 cgacgtctgc gccttcctcg tcagggaggg cgtcgccgtc ttcagctgga gggggatgag
     6241 cgggggggag tatttcgaaa acctggagtc cgtcctggac cgctcgccgg agatcctcgt
     6301 cgacgacggg gccgacctgg tggtgacggc ccacgagaaa tacccggcgc tccttccccg
     6361 ggtgaaaggc gggtccgagg agaccaccac cggcgtcagg aggctcaagg ctatgatgga
     6421 ccagggggtc ctggccttcc cgatgatctc cgtcaacgat gccaggagca agttcctctt
     6481 cgacaaccgc tacgggacgg gccagtccgt tttcgacggg ctgatgaggg ccaccaacgt
     6541 cctcatagcc ggcaaggtct tcgtcgtggc gggctacggc tggtgcggcc gcggcgtcgc
     6601 catgagggcc aagggtttcg gcgccagggt cgtcgtcgtc gagactgacc cccacagggc
     6661 cttcgaggcc ctgatggacg gatgccaggt ctgcgatatg atggaggccg cgtccatggg
     6721 cgacctgttc ctgaccctca ccggcaacag ggacgtcatc agggaggagc acttccgggt
     6781 catgaaggac ggggccatcc tgggcaacgc cggtcatttc gacgtagaga tcgacaaggc
     6841 cgccctcgag aggctctcga aagaagtctt cgaggccagg gacaacgtca ccacctatgt
     6901 cctcggcgac ggcaggagga taagcctcct cgcccagggg aggctcgtca acctcgccgc
     6961 cggcgacgga caccccatag agatcatgga cctcagcttc ggccttcaac ttgggtccgt
     7021 cctgcacctg gccgaaaacc ggctctcccc cggcgtttac gacgtccccg gtgagataga
     7081 cgccgacgtg gtcaggacgg ccctcaaggc gaggggcatc agcctcgagg tgccgaccag
     7141 ggaacaggaa gagtaccttc gccagtggag ggagtgaggg accttggcgg tccttttcag
     7201 ggatttttat tacatcgatt ccaagatgga agggggggcc cggggcgacc tcctggtgga
     7261 gggcggcagg atagcgcgcc tggggaaaga ccttcccggg ccttctggcg gtgagacgat
     7321 caggggcgag gggaaggccc tcctggcgcc gggcttcgtc aacggccaca cccacgccgc
     7381 catggtcctc ctccgggggc tgggggaaga actgcccgtc atggagtggc tccaggagag
     7441 gatatggccc gtcgaggcgg ggctcctccc ggagcatatc tactggggga ccaggggggc
     7501 cgtcatggag atggcctcca cagggacgac ctgtttttgc gacatgtact tcgagatgga
     7561 cgatgtggcc agggctgcct ccgagagggg catgcggtgc tgcctgagcc gggggctcac
     7621 cggcgacgac cccgccaagg tccgggaagg ggcggaactc ttccgccgct ggcacggcag
     7681 ggaccatgtc acggtgcagc tggggcccca cgctccctac acggtctccc tggaagcgat
     7741 gaagggcata acggccctgg ccgaggagat aggcgcgggg gtgcactttc acttcctcga
     7801 ggccgagtgg gaaccctcct tcatcaggga taagtacggt ctctcccccc tggcttacct
     7861 ggaggaggcc ggtctcctgg gctcatgtcc cgccatcctg gcccactgcg tctggttccc
     7921 ccctttcgag atacccggcc tggccggaac cgccgcgacg gtggcccaca acccgtcgag
     7981 caatatgaag ctgggcagcg gccacgcccc ggcccaggct ttcctggacg gcggcgttcc
     8041 cctctcgctc ggcaccgacg gggcggcgag caacaaccgg ctcgacgtct gggaggagat
     8101 gaggctctgc gcccttctgc acaagggagc cctgaaggac ccgaccgtca tggcctcccg
     8161 cgacgtcttc cggatggcga ccctatcggg ggcggcctcg gcggggttca gcgatgtggg
     8221 gcttctccgg gagggctggc aggccgacct ggtcatgatc gacctggacc ggcctcatta
     8281 catagggtgg gaccgcgaaa atatcgtcga tttcctcgtt tacgccggct tcagttccga
     8341 tatacttggt acgatgtgcg ccgggaagtg gctctaccgg gatggtgagt ttcccggcca
     8401 ggactacgac gccgtgatgg gcgaggtgac ccggtgcagg gaggacctgt tgcgccgcgc
     8461 cgcccggcgg cagccgtagg ttcgccgagg cagacggggg agagaacgcc gaagatgcga
     8521 tggagaagcg gtaaggaact gagggagatg ttcttatcct atttcgagag gaacggccac
     8581 aggaggttcc ccagcttttc gctggtgccc gatgacccga cgctgctctt caccatagcg
     8641 gggatggtcc ccttcaagcc ctacttcctg ggcaagcggg aaccctcctt caaaagggcg
     8701 accacctccc agaagtgcgt caggaccaac gacatagaga acgtgggcag gacggcgagg
     8761 caccacactc tcttcgagat gctcggcaac ttcagcttcg gcgattactt caagagcggc
     8821 gccgccgcct tcgcctggg
//
LOCUS       DAOSRG010000036         8785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_7608_length_8785_cov_9.790149, whole genome shotgun sequence.
ACCESSION   DAOSRG010000036 DAOSRG010000000
VERSION     DAOSRG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7608_length_8785_cov_9.790149"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..2647
                     /locus_tag="PLM30_02540"
     CDS             263..2647
                     /locus_tag="PLM30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOR54080.1"
                     /translation="MKNNIFNFFKKKQPQRTTKNDRTSRGTSRRSAPRKSGFLGKLAL
                     ILAFFVIFLFAVVSVVGVLLIRDAASTLPSLEEMANYEPDLSTIVYDRNEKVITRLFH
                     ENRTWVPLSQIPLVMQEASIAAEDSAFYSHRGLDFVGIIRAFWVNFRSGKTVQGASTI
                     TQQLAWNLFLTKEKTLQRKIKEAILSVRLEKIYSKEQILEMYLNMIYFGHGAWGIHSA
                     SLLYFNKEPSELDLSEAALLAGIVKGPEYYTPLRHPERASERQAYVLGRMIHEGMITP
                     EQAQGARRIELDFQTLKKPAIMFDEAPYFIAHILFEQLLPTYGSDMVYRGGLRVHTTL
                     DLDVQHAAEEAIKGLKSEGAIVAIDPGTGEILAMVGGKDFEQSKFNRATQAFRQPGSS
                     FKPFVYTAALMKGLRPVDHVLDAPVSYDNGIDERGERDIWEPGNYTKEYKGEVTLLQA
                     LVHSYNTSAVRVAHITGIPGIIDLARRFGFTSPHIPNDLSLALGSANVTPLEMASAYS
                     VFANGGSKIQPYSIRRVSTAAGEVLEQYGPTLSPAVPEEIALSMRSVLMEVVRSGTGT
                     RARVPGYEVFGKTGTTNGYTDAWFAGGVPGLVVVVYAGNDDNKSLGKTATGGVIAAPV
                     WQAFVSKAVKYLTLKENFEVPEEAMVDAVTICKDSGFLSAEGCPSTVILLPSGEAPLS
                     TCPLHGGSWIMAESDTNEPKMLLSPRDEEVLGVPAQMARLYSISVSDMTAAAALPAEE
                     PLFYERPQAPSFAPPPAPTGAKKPTSTPTRPRERTPKEIEDRFQELLKEYGISD"
     gene            complement(2663..3691)
                     /locus_tag="PLM30_02545"
     CDS             complement(2663..3691)
                     /locus_tag="PLM30_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOR54081.1"
                     /translation="MPREAPRIRWERIERIILDPCAAFSDESRGRMREEPLCLLRTIY
                     QQDRDRIIHSKAFRRLKHKTQVLLLPEGDHYRTRLTHTLEVCQVARTIARALALNEDL
                     TDAIALGHDLGHTPFGHMGEKVLDRIAKENGLAGFSHARQSLRVVDCLEKDGRGLNLS
                     MEVRDGILKHSKGQVDVREGFHGGEPVLTNEARVVRISDSIAYLNHDLDDALRAGIVR
                     NEDIPPSVTSKLGVTHGERIGRMVESVVSNSGCGGVRMGDELLEVVEVLRSFLYDRVY
                     ASERVLREEPRVTYVLSALFSHFLDNPPETCCFGDTALAALDHISGMTDRYALAFFER
                     TILPSPWP"
     gene            complement(3774..>5240)
                     /locus_tag="PLM30_02550"
     CDS             complement(3774..>5240)
                     /locus_tag="PLM30_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOR54082.1"
                     /translation="ASPGAAVGIAVFDADEAEKVAQSGQPVILVRPETTPDDIHGLYA
                     AQGVLTSRGGMTSHAAVVARGLGKPCVSGCESLTIDAAKGVMTVGGRSLKKGETLTID
                     GSKGHVIFGEVSLVQPSFTDDFRELLDIADSVSALEVWANADTPDDAQRARSFGARGI
                     GLCRTEHMFMAAGRLPVMHEMILAKDRQSRVRALEVLKGMQKEDFRGIFRAMEGFPVT
                     IRLLDPPLHEFLPKEHELRETISSIKADSEASPEDLVHAEAVLARVLSLQENNPMLGF
                     RGCRLGLVYPEIYEMQIAAIIEAACELKKEGVDVRPDIMMPLVGTREEMKRLLSMVND
                     IAGGIIPRLGVEDLHYLVGTMIEVPRAALVAGDIAEFAEFFSFGTNDLTQTTFGYSRD
                     DAEGKFLAQYVNDGVLPDNPFHELDREGVGRLMSIAATEGRNKRPRIQIGICGEHGGD
                     PKSIAFCHQLGLDYVSCSPFRIPVARLSAAHSQLGLLD"
     assembly_gap    5241..5340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5341..6290)
                     /locus_tag="PLM30_02555"
     CDS             complement(<5341..6290)
                     /locus_tag="PLM30_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOR54083.1"
                     /translation="MTKTGDERKRYVFHFTEGSAEKRSLLGGKGANLAEMLKIGLPVP
                     PGFTISTEACLGYLEKGSQFLDEVWGEIRVAMTDLEKETGKTFGGNSNPLLVSVRSGA
                     AVSMPGMMDTILNLGLNADSVKALAAESGDERFALDSYRRFIQMFSNVVLGLEGAVFE
                     NVLHQVKKDLGVTYDFQVPPSAWKGVISRYLKIVEQETGAPFPEDPWVQLKRSVEAVF
                     LSWKNPRAITYRRINRIPDDLGTAVNVMAMVFGNLGDDCGTGVCFTRNPSTGEKKLYG
                     EFLVNAQGEDVVAGIRTPNPIDEFGSRLPEADRELHRIAEL"
     gene            complement(6323..7144)
                     /locus_tag="PLM30_02560"
     CDS             complement(6323..7144)
                     /locus_tag="PLM30_02560"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOR54084.1"
                     /translation="MEGKGDHQPLPVFVVSDFTGETAEHVAKAALSQFATDTITLQRF
                     RYINNKDRALEVLEEAEGEKAMIVCTLVDHELRRWFVEKADLQGTDVIDILGPILDML
                     EARLGHAPLETPGLMRRMDEEYFRRVKAIEFAIKCDDGRCAEHLLSADVVILGVSRTS
                     KTPLCMYLAHRGFMVGNVPLIPETEPPGEIFEISPERIFGLTVQPEKLVDIRKERLRL
                     LGLDSDISNYAQWERVEEEIQFARNIMRKIGCRVLNVTNRAIEETAQEILDFLRA"
     gene            complement(7184..>8785)
                     /gene="glyS"
                     /locus_tag="PLM30_02565"
     CDS             complement(7184..>8785)
                     /gene="glyS"
                     /locus_tag="PLM30_02565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOR54085.1"
                     /translation="RFARPVRWLLALYGDELLRVDFGLVSSDRFTRGHRFMGVRKIEV
                     SKVGEYLEKLHDNFVIADPQKRREKMLAGIASIEKEMGGKAALDADLVEENLYLVEYP
                     VPFYGFFDRSFLQIPEEVLVTTMKNHQRYFPVRDCSGNLKPCFIGVSNNRAANMSVVR
                     EGNERVLRARLSDASFFWEEDQKVPLSARVDSLKKIVHHEKLGTVYEKVSQVRELCGA
                     LCRMMDLPAEETEMVDRAAFLAKADSVTDMVYEFPELRGVMGREYAARDGENPRVALA
                     LFEQYLPSSSGDRVPSDIIGAILGVCDRIDTITGGFKAGLQPTGSQDQYGIRRASRTL
                     NEILWGMDLDVDIDLLVKTSSLSHRLSDDQKVAASGFILQRLYNQLREKGFSHELTTL
                     AVSAVGSRPNQALKLLKAFQEVQGTDWFAGLVTSAVRVRNILQKTDHSARTLDASLFT
                     EAAERELHEAVELLSPRVDEAVRAWDWNGLTLVLSKLEPYISAFFDKVLVMDNDERIR
                     NNRIALLERCDGLFKTSGDLGLLKA"
BASE COUNT         1719 a         2685 c         2467 g         1814 t
ORIGIN      
        1 gtcaggcacg ccgccagcgt tcgtcctgag ccaggatcaa actctcaaaa aaatatccga
       61 ggtcattcgc acatgtattc ctgtacgctg tattcttttt tcaaggtacc gccgccttca
      121 tgggcgacgg cgaaaaatat accatgcatc ggcagtaagc gcaagcgctt gataaacagg
      181 aacttcgtaa aatgcccttt ttttggtatt cttctatggt ccgttgttca cttgggagct
      241 cacggttaaa ccaggaggcg gcatgaagaa caatatcttc aatttcttca agaagaaaca
      301 gccccaaagg accacgaaga acgatagaac ctcccggggg accagcagga ggtccgctcc
      361 caggaagtcc ggttttctcg gcaagctggc cctgatcctt gccttctttg taatattcct
      421 tttcgccgtg gtttccgttg tgggcgtcct gctcatcagg gacgcggcct cgaccctccc
      481 ttctttggag gaaatggcca attatgaacc ggacctgtcc accatcgttt acgaccggaa
      541 cgaaaaagtc atcaccaggc tttttcatga gaacaggacc tgggtacccc tttcccagat
      601 ccccttggtg atgcaggagg cctccatcgc ggccgaggac agcgcctttt acagccacag
      661 gggcctcgac ttcgtcggga tcatcagggc cttctgggtg aacttccgga gcggcaaaac
      721 cgtccagggc gccagcacaa taacccagca acttgcctgg aacctcttcc ttaccaagga
      781 gaagaccctc cagagaaaga tcaaggaagc gatcttgtcg gtccggctgg agaagatcta
      841 ctccaaggag cagatcctcg aaatgtacct caacatgatc tacttcggcc acggggcatg
      901 ggggatccat tccgcctcgc tgctatactt caacaaggaa ccctcggagc tggacctctc
      961 cgaggcggcc cttctcgcgg ggatagtcaa gggtcccgag tactacacac ccttgcggca
     1021 ccccgaaagg gcatcggaga ggcaggccta cgtcctgggc aggatgatcc acgagggaat
     1081 gataacgccg gaacaggccc agggggccag gaggatcgaa ctggatttcc agaccctcaa
     1141 aaaaccggcc atcatgtttg acgaggcgcc ctacttcatc gcccacatcc tttttgagca
     1201 actcctcccc acctacggca gcgacatggt ctaccgaggg gggctcaggg ttcacacaac
     1261 ccttgacctg gacgtacaac acgcggccga ggaggccatc aagggtctga agagcgaggg
     1321 ggccatcgtg gctatcgacc ccggcacggg agagatcctg gccatggtag gcgggaagga
     1381 tttcgagcag tccaagttca accgagcgac ccaggctttt cgccagcccg gttcgtcctt
     1441 caaacccttt gtgtacacgg ctgccctcat gaaggggcta cgaccggtgg accatgtcct
     1501 cgacgccccg gtttcctacg ataacggtat agacgaacgg ggagaacggg atatctggga
     1561 accgggcaac tacactaaag agtacaaggg ggaggtgacg ctgctccagg ccctggtcca
     1621 ttcctacaat acctcggcgg tcagggtggc ccatatcaca ggcataccgg ggatcatcga
     1681 cctggcgcgc cggttcggct tcacatcgcc ccacatccct aacgacctct cgctggcgct
     1741 gggttccgcc aacgtcaccc ccctggagat ggcatcggcc tacagcgtct tcgccaacgg
     1801 gggttcgaag atccagccct actccataag gcgggtcagc accgccgccg gcgaggtgtt
     1861 ggagcagtac ggccccactc tctctccagc cgtccccgag gagatcgccc tttcgatgcg
     1921 ttccgtcctg atggaggtcg tccggtcggg aacgggaaca agggccaggg tcccggggta
     1981 cgaagtcttc ggcaagacgg ggacgaccaa cgggtacacc gacgcttggt tcgcgggggg
     2041 ggtgccgggc ctcgtggtgg tggtgtacgc cggcaacgac gataacaaat ccctgggcaa
     2101 aaccgctacg ggcggcgtga tcgcggcccc ggtctggcag gcctttgtca gcaaggcggt
     2161 gaagtacctg accctcaagg agaacttcga ggtacccgag gaagcgatgg tggatgccgt
     2221 gactatatgc aaagattccg ggttcctgtc ggcggagggt tgcccttcga cggtcatcct
     2281 ccttccttcc ggtgaagctc ccctttctac ctgtcccctt catgggggtt catggataat
     2341 ggccgaatcc gatacaaacg agcccaagat gctgctttcg cccagggatg aggaggtcct
     2401 cggcgtacct gcccaaatgg ccaggctcta cagtatctct gtttcggaca tgacggccgc
     2461 tgccgctctc cccgcggagg aaccgctctt ttatgagaga ccgcaggcgc cctccttcgc
     2521 accacccccg gccccaaccg gtgccaagaa gccgacctcc accccgacta gaccgaggga
     2581 gagaaccccc aaggagatcg aggacaggtt ccaggaattg ctcaaggagt acggcatctc
     2641 cgattagaag atcgcgggga tctcacggcc aggggctggg gaggatcgtc ctctcgaaga
     2701 aagccagggc gtacctgtcg gtcatgcctg aaatgtgatc cagcgcagca agggcagtgt
     2761 caccaaagca gcaggtctcg ggcgggttat caagaaaatg gctgaaaagc gcggatagta
     2821 cgtaggtcac tctgggttcc tcgcggagca ccctctccga agcgtagacc ctgtcgtaaa
     2881 ggaaagaacg gagtacctcg acgacctcaa ggagttcgtc gcccattcgc accccgccgc
     2941 agccgctgtt ggagacgacg ctttcgacca tcctgcctat cctctcgccg tgggtcaccc
     3001 cgagcttgga tgtgaccgat ggtggtatat cctcgtttct gacgatgccc gccctcagcg
     3061 cgtcgtcaag gtcgtggttc aggtaggcaa tggagtcgga gatccggacg acccttgctt
     3121 cgttggtcag gaccggttct ccgccgtgaa acccctcccg cacatccacc tgccccttgg
     3181 agtgtttcag gatgccgtcg cggacctcca tcgacaggtt caacccccgg ccgtcctttt
     3241 cgaggcagtc gaccaccctc aggctctgcc tggcatggga gaacccggcg agcccgtttt
     3301 ccttcgcgat cctgtccagt accttttcac ccatgtggcc gaagggtgtg tgcccgagat
     3361 catgcccaag ggcgatggca tcagtcaggt cctcgttaag ggccagagcc ctggcaatgg
     3421 tcctggccac ctggcagacc tccagggtat gggtgagcct cgtcctgtaa tggtccccct
     3481 cggggaggag caacacctgg gtcttgtgtt tcaacctcct gaaggctttc gagtggatta
     3541 tcctgtcccg gtcctgctgg tagatggtcc tcaagagaca aagtggctct tcccgcatcc
     3601 ttccccggct ctcatcactg aaagcagcgc aggggtcgag gatgatcctc tcgatcctct
     3661 cccatcggat cctgggtgct tctcttggca tgatgcctcc ttcgtgagtg gggccgccac
     3721 ttcgagcggc ggccccaggg tctacaaaac cttgtcctct cacgtcccga agctcaatcc
     3781 aggaggccca gctgcgaatg ggcggcggaa agtctagcca cgggtatccg gaagggggaa
     3841 cagctgacat agtccaggcc cagctggtga cagaaggcga tgctcttggg gtccccgccg
     3901 tgttcgccgc atatacctat ctggatccgg gggcgtttgt tccttccttc cgttgccgcg
     3961 atggacataa gcctgccgac cccttctcgg tccagttcat ggaaggggtt atcgggaagg
     4021 acaccgtcgt tgacgtactg ggccaggaat ttgccctccg cgtcgtccct ggaatagccg
     4081 aaagtggtct gggtgaggtc gttggtcccg aagctgaaga actcggcgaa ttcggcgata
     4141 tcccccgcga cgagggccgc ccttgggacc tcgatcatgg tcccgaccag gtagtgaagg
     4201 tcttccacac caagccgcgg gatgatgcct cccgcgatgt cattcaccat cgacagcagc
     4261 ctcttcatct cttccctggt ccccaccagg ggcatcatga tatcgggcct gacatccact
     4321 ccctccttct tgagttcgca ggccgcctcg atgatcgcgg cgatctgcat ctcgtagatc
     4381 tcggggtaga cgagtcccag gcggcatccc ctgaaaccga gcatggggtt gttctcctgg
     4441 agcgacagga ctctcgccag gaccgcttcc gcgtgcacca ggtcctccgg tgaggcttcg
     4501 ctgtcagcct tgatggagga aatggtctcc ctgagctcgt gttccttcgg aaggaactcg
     4561 tggaggggcg gatcgagcaa ccttatggta accgggaacc cctccatggc cctgaagatc
     4621 ccccggaagt cctctttctg cattcccttg aggacctcga gcgcccttac cctggactgt
     4681 ctatccttgg cgaggatcat ctcatgcatc acggggagcc tccccgcggc catgaacata
     4741 tgttccgtcc tgcaaagacc tatacccctg gcgccgaagg acctcgccct ctgcgcgtcg
     4801 tcaggggtat cggcgttcgc ccagacttca agggccgaca cggaatcggc tatatccagc
     4861 agttccctga aatcatcggt gaaggagggc tggacaaggg acacttcgcc aaagatcacg
     4921 tgcccctttg acccgtcgat ggtgagggtc tcaccctttt tcagtgatcg gccaccgacg
     4981 gtcataacac ccttagcggc gtcgatggtc aacgattcac accccgaaac gcagggtttc
     5041 ccaagccccc tggcgacaac ggcggcatgg ctcgtcatcc cgcccctgct ggtcagaacc
     5101 ccctgggcgg cataaagacc atggatatcg tcgggggtcg tctccggcct cacgaggatg
     5161 accggttgcc ctgactgggc caccttctcg gcttcatcgg catcgaaaac ggctatgccc
     5221 accgccgctc ccggtgacgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 agctccgcga tccggtggag ttccctgtcg gcctcgggca gccgtgaccc gaattcgtcg
     5401 atggggttcg gggtcctgat gccagctacg acatcctcgc cctgggcgtt cacgaggaac
     5461 tcgccgtaca gttttttctc ccccgttgaa gggttcctgg tgaagcatac gcccgtcccg
     5521 cagtcatccc ccaggttgcc gaagaccatg gccatgacgt tgacggccgt gcccaggtcg
     5581 tcggggatcc tgttgatcct cctgtaggtg atggccctgg ggttcttcca actgagaaag
     5641 accgcctcta cggatctctt cagttggacc cacgggtctt ccggaaaggg agcgcccgtc
     5701 tcctgctcca ctatcttgag gtaccgtgaa ataacgccct tccaggcgga tggaggtacc
     5761 tggaaatcgt aagtgacccc gaggtccttc ttgacctggt gaaggacatt ctcaaagaca
     5821 gccccctcca agcccaggac cacgttggag aacatctgga tgaacctcct gtagctgtca
     5881 agggcgaacc tctcatcgcc cgactccgcg gcgagggcct tgacggagtc ggcgttgagg
     5941 ccgaggttca gtatggtgtc catcataccc ggcatggaga ccgccgcccc cgaacggacc
     6001 gagacgagaa gcgggttgga gttcccgccg aaggtcttgc ccgtctcctt ttcgagatcg
     6061 gtcatggcaa cccggatctc accccacact tcgtccagga attgactccc cttttcaagg
     6121 taacccaggc aggcctcggt cgaaatggtg aaccccggcg gaacggggag gccgatctta
     6181 agcatctccg ccagattggc ccccttaccg cccagaagag atctcttttc ggcgctgccc
     6241 tcggtgaaat gaaaaacgta tctttttctt tcatccccgg ttttcgtcat gctttccgac
     6301 ctccacggca tttagggttt gatcatgctc tcaggaaatc caggatctcc tgggccgtct
     6361 cttctatggc cctgttggtg acgttaagga cacggcaacc gatcttccgc atgatgttcc
     6421 tcgcgaactg gatctcctcc tcaaccctct cccattgtgc gtagttggag atatccgaat
     6481 cgaggccaag gaggcgcaac ctctccttcc tgatgtccac gagtttctca ggctggaccg
     6541 tcaggccgaa gattctctcg ggggagatct caaagatctc gccggggggc tccgtttcgg
     6601 gtataagggg cacattgccc accatgaagc cgcggtgggc gaggtacatg cagagggggg
     6661 tcttgctcgt cctggagacc cccaggatga ccacgtccgc cgacaggagg tgttcggcgc
     6721 acctcccgtc atcgcacttg atagcgaatt cgatggcctt caccctccgg aaatactcct
     6781 catccattct tctcatcagg ccgggggtct ccaggggggc gtgaccgagg cgtgcctcga
     6841 gcatatccag gataggcccg agtatatcga tcacatctgt cccctggagg tccgccttct
     6901 cgacgaacca acgcctcagt tcgtggtcga ccagggtgca gacgatcatg gccttttcac
     6961 cctcggcctc ttcgaggact tccagggctc tgtctttgtt gttgatgtaa cggaaccttt
     7021 gaagcgtaat ggtgtccgtc gcgaactggc tcagagccgc ttttgcgaca tgctctgccg
     7081 tctctcccgt gaaatcagag accacgaaaa cgggcagcgg ctgatggtca cccttccctt
     7141 ccatgacaag tcctccacgg tcgatctgga aactcggctg tcgtcaagcc ttcagcagcc
     7201 ccaggtctcc ggatgtcttg aacagcccgt cgcatctttc caggagggct atcctattgt
     7261 tcctgatacg ctcatcgttg tccatcacca gcaccttgtc gaaaaaggcg gatatatagg
     7321 gctccagttt agaaaggacc agggtcaggc cgttccagtc ccacgccctt accgcctcgt
     7381 ccacccgggg ggatagcagt tcgacggcct cgtggagttc cctttcagcg gcctcagtga
     7441 aaagggaggc gtccagggta cgcgcggaat gatcggtttt ctgaaggata ttcctgaccc
     7501 tgaccgccga cgtgaccagg ccggcgaacc agtctgtccc ctggacctcc tggaaggcct
     7561 tcagcagctt caaggcctgg ttgggcctcg atcccaccgc cgagacggcc agcgtggtca
     7621 gttcatggga aaagcctttc tcgcggagtt ggttgtagag cctctgcagg atgaaccctg
     7681 aagcggctac cttctgatca tctgaaagtc tgtgcgacag ggaggaggtt ttaaccagca
     7741 agtcaatatc tacatccagg tccatgcccc agaggatctc gttgagcgtc cgggaggccc
     7801 tacggatacc gtactggtcc tgggaccccg tcggctggag acccgccttg aacccgccgg
     7861 tgatcgtgtc gatcctgtcg cagaccccca ggatggcgcc gatgatgtcc gagggtaccc
     7921 tgtcgcctga ggaggacggc aggtattgtt caaagagcgc aagggccacc ctggggtttt
     7981 ccccgtccct ggcggcgtac tcacggccca taacacccct cagttcagga aattcgtaga
     8041 ccatgtcggt gacgctgtcg gccttcgcga gaaaggcggc gcgatcgacc atttccgtct
     8101 cctcggctgg aaggtccatc atccggcaga gagctccgca aagctcccgg acttgggaca
     8161 ccttttcgta gaccgtcccg agtttttcgt ggtgaacgat cttcttgagg gagtccaccc
     8221 gagccgataa aggcaccttc tgatcctctt cccagaagaa ggacgcatcg gaaagccttg
     8281 cccttaggac cctctcgttc ccttccctca cgacggacat attggcggca cgattgttgc
     8341 tgacacctat gaagcagggc ttcaagttac ctgagcagtc ccttacgggg aaatatcgtt
     8401 ggtgattttt catcgtggtg acgaggacct cttcggggat ctggagaaag gatcgatcga
     8461 aaaagccata gaacggcacg gggtattcga cgaggtagag gttctcttcg acaaggtcgg
     8521 cgtctagggc cgccttgccg cccatctctt tctcgatcga ggcgatgccg gcgagcattt
     8581 tttcacgcct cttttgcggg tcggcgatga cgaagttgtc gtgcagcttc tcgaggtact
     8641 cccccacctt tgaaacctcg atcttcctga cacccatgaa gcggtgtccc ctggtaaagc
     8701 ggtcggagct cacgagcccg aagtccaccc tcagcagttc atccccataa agggccagaa
     8761 gccatcggac gggcctggcg aaacg
//
LOCUS       DAOSRG010000037         8556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_7863_length_8556_cov_7.746030, whole genome shotgun sequence.
ACCESSION   DAOSRG010000037 DAOSRG010000000
VERSION     DAOSRG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7863_length_8556_cov_7.746030"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLM30_02570"
     CDS             complement(<1..404)
                     /locus_tag="PLM30_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54086.1"
                     /translation="MRKRNNVLKVGILALMAFALVASCFQTGAIAAETKEKTWQWYPS
                     TWQPSGVVWDRLVYISDLITRGSGGRIKVTPTAPGMVCAVAEQLDAVATGATPAMMIY
                     TDYYSGKMPLLGMTTNPLFLLNNTWELHQYVE"
     gene            complement(537..1274)
                     /locus_tag="PLM30_02575"
     CDS             complement(537..1274)
                     /locus_tag="PLM30_02575"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR54087.1"
                     /translation="MTSQKGHAVVTGGGSGIGRAIARTLASEGWSVSVLDLSEERAQA
                     VADEITRDGGTARGIACDLSTRDGAQGGVLKARSALGPVKALVNNVGIYPSGPFVEIK
                     DQDWDLVMNVCLKSFLWCSQESLVDMMAQRSGKIVNMASIDGKTPGPGNAAYSAAKAG
                     VISLTRSLAAEMAEFNINVNAVAPGWVETPNILKSDRWKEAIKKIPIGRLAKPEEIAD
                     AVSFLLSEKAVYITGEVLNINGGMLMD"
     gene            1730..2446
                     /locus_tag="PLM30_02580"
     CDS             1730..2446
                     /locus_tag="PLM30_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOR54088.1"
                     /translation="MSKRGALVNQILEGIKNGSLVENGQLPSERALANQLGISRILLR
                     ETIVVLETLGVVESKERLGIFVKKPDISGLAESLRIMPYWSESFVPQLIEIRLMTDVQ
                     AAELAALRRTEKQLEELRQCLATFLEIGVDTPQEAKLSSHYEYIFHRLIVAAANNDIL
                     IRVYEGLSALMEKNNDFLHEKLTRDTTWGKRVKEHHRNIFQAIQDEDPEKAKQWMRTH
                     LFETRDRYGKILEELKGLFI"
     gene            complement(2614..3354)
                     /locus_tag="PLM30_02585"
     CDS             complement(2614..3354)
                     /locus_tag="PLM30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOR54089.1"
                     /translation="MARYKVRSRTRSWDGVPIGILILDACYPCIPGNVGNATTYDFPV
                     RYKEVSGASSEKLINQRDPSLLELFIKAAQELEREGVRAITGACGFMALFQRQVAQAV
                     HIPVFISSLLQVPFVHRILPPGRSVGIITANKAQLTSEHFESVGITPDMPVVVYGMED
                     QDEFRTSILDEKGTLDSDLIEKEVCGVASRMKEENPNLGAVLLECSDLPPYAAAIQEV
                     TDLPVFDFITMINHVHSAVCRRRYSGFM"
     gene            complement(3583..5280)
                     /locus_tag="PLM30_02590"
     CDS             complement(3583..5280)
                     /locus_tag="PLM30_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54090.1"
                     /translation="MDGPVPERTFIKPGRPRITTTYCLKAHTGHLLKEGEDPFFRTIE
                     ESVRWLAQKVPTPLPEAAGQGESFVLEEPGQRLECVSIPEKGVWAVRFSHPDAGMGEL
                     RAVPGRTWLSDLSVTKTDRGILFGVLISCSSLPDRDDFVAFIRPGIIKSLADTVGLSQ
                     VRPLDGEPWGVRTSAEVGRLFELVFAPERTIPVIVATQPDRSKWTLPGEFPEWTIDCR
                     ELARKTLGYAHVACLARDASFEWTDRVGKIWSVFDGAVRTYMPGIDQEKDSPRSHPLA
                     MKDRIYYWRYRDGFGESAFVEEVIAKTRVANPRVQFEWKPLVFLAEARLVQADLRVRE
                     LKERLGEKDCEKCIAEFDELREAMQARIDESEESAAEMAALATDYASEADYHRNRGNT
                     LAAALEALRSAMKVPVDDPDEEVPIPHDYEAMPQWVEKNLAGRVELLPRARNALKKAR
                     YKGVELVYRALLLLATEYRDMRYGMLSLADFEGCCRELGLEHRPAIREKSAGEQGDEY
                     YVSYPPGTSSRRLLDTHLAKGVSRDERETLRIYFFWDEESSQVIVGWLPSHLDTRCS"
     gene            6085..6537
                     /locus_tag="PLM30_02595"
     CDS             6085..6537
                     /locus_tag="PLM30_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54091.1"
                     /translation="MRSSSSWDKPERAPNFIARKRSDWSLFRRGTHIPLEFLPDFEKA
                     NGNYRLKRGEAREVILLYQDRFFQATICNIDRPTKSETVQIRFDGNNELKALLREELK
                     KSFNHLEQKGPHTRVPPDLAEYVEFYKTGIPFVYRILFIKIGDSLFNL"
     gene            complement(6903..7805)
                     /locus_tag="PLM30_02600"
     CDS             complement(6903..7805)
                     /locus_tag="PLM30_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54092.1"
                     /translation="MKKTLVFFTLLTLLAVSPAFSFNEPESYDDYRWGQPFSEFQDLS
                     DYEGSDEEEGQLTSARADGVARTWKGLKFIESPLFLFRQLRFEGFLAEGRPGRAEWET
                     WCEVLERELGTPSRGSDELNYVRWESDRHWVEIVHNPDDESNLVSAGAYLEGLDDGES
                     CPPALEDPFEDGFKGLKWGAKSYGTVNIQFISRDHDLNISFYLGQGDLEVSELGGIPM
                     KKGAFLYGFINSTFSMLRGTALYKDREAFEGLKAFFESFLGPVDTYSPNVNSLWERDR
                     YKVFLYREKDGSVAFWLVNKEYHR"
     gene            complement(8094..>8556)
                     /locus_tag="PLM30_02605"
     CDS             complement(8094..>8556)
                     /locus_tag="PLM30_02605"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOR54093.1"
                     /translation="STFTPFEGFDRIIMTIDNDLALSHDDGEEITLKKFEPHLFDGGA
                     ETRSRGKVRDFNCIMDRKTCRGETTAMTLSKGSVLYPRKGNYGAGLELEIIYCAAGRL
                     TFRYGETEERLDQGDVLAIDHGLMNSRYRLSNEEDQPCDLVVTLIRSVPPC"
BASE COUNT         1838 a         2492 c         2387 g         1839 t
ORIGIN      
        1 ttctctacat actggtggag ctcccaggtg ttgttaagca ggaagagggg gttggtggtc
       61 atacccagga ggggcatctt tcccgaatag tagtcggtat agatcatcat cgccggggtt
      121 gccccggtgg ccacggcgtc cagctgctcg gcgacggcac agaccatccc cggagcggtg
      181 ggcgtcacct tgatgcggcc gcccgaaccg cgggtgatga ggtccgagat gtagacaagg
      241 cggtcccata cgacgccgct tggctgccag gtcgaaggat accactgcca ggttttttcc
      301 ttagtctcgg ccgcaatggc tccggtctgg aaacaggagg caacgagagc gaaagccatg
      361 agtgctaaaa tcccaacttt cagtacgttg tttctctttc tcattacgaa gagcacctcc
      421 tcagtttact ggcgtttctt attacacagg tagttttgat ccgattcgat ggcatccccc
      481 ctgctccggc ctgatgaggc tttgctcact gtaatcgctt cggggaatga actcgatcag
      541 tccatcaaca ttccgccatt aatgttgagg acctctcccg taatataaac ggccttctcg
      601 gaaaggagga aggaaacggc gtcggctatt tcttcgggtt tggccaaacg cccgatgggg
      661 atcttcttga tcgcttcttt ccagcggtcg cttttaagga tattgggcgt ttccacccat
      721 cccggcgcga cggcgttcac gttgatgttg aattccgcca tttccgctgc cagggatcgg
      781 gtcaaactga tcaccccggc cttggccgcc gagtaggcgg cgttccctgg tccaggcgtc
      841 ttcccgtcga tggaggccat gttgacgatc ttccccgatc gctgggccat catatcgacg
      901 aggctctcct ggctgcacca taagaaagat ttcaggcata cgttcatgac caagtcccaa
      961 tcctggtctt ttatttccac gaaggggccg ctggggtaaa tacccacgtt gttgacgagg
     1021 gccttgacgg gtccgagggc ggaccgcgct ttcaagacgc ctccctgggc accatcccgc
     1081 gtcgagaggt cgcaggcgat tcctcgggcg gtcccgccat ccctggttat ctcatccgcc
     1141 accgcttggg cgcgctcttc tgagaggtcg agaacgctaa cggaccaccc ttcactcgcc
     1201 agcgtccggg caatggccct ccctatgccg cttcctcccc cggtgacaac agcatgaccc
     1261 ttttgtgatg tcaaaaatcc cacccccaga ataggtagac tattagttcc gaggaaaacc
     1321 ccgatggtga tcacggtttc cgaaccgtcg aagccgtgat aaaacgctga tcggggaccg
     1381 ccgcgttccg tccgccggcc ccgggatact cgcaaaagat ctacttcttt ggggtacgaa
     1441 gggctttcgt aaaaaatggt atcattggat ttaccaagat aataccagtt tgggaaacat
     1501 tttaacatgc cgtcggataa cgtcaagaaa gggggccgcc aaggcctttc atgtatactg
     1561 atgctcggga acgcacccgg agcggacccc ggggggcggc agggtttccg ggtaaaacgg
     1621 gagatccggg gaaaaaggac acccgccgtg gaagacacgc ttcaaacctt tcgaggcctg
     1681 tcaccgcggg tttgcgcggt cggtcagaaa aatttaaggg ggaactacag tgagcaagcg
     1741 aggagccctg gtcaatcaga tactcgaagg aataaaaaac ggttccctgg tcgagaacgg
     1801 ccaattgccc tcggaaaggg ccctggcgaa tcagctcggc atcagccgca tcctccttcg
     1861 agaaaccata gtggtcttgg agaccctggg tgtggtagaa agcaaggaga ggctcggaat
     1921 tttcgtcaag aaacccgaca taagcggcct ggcggaatcc ctgcgtataa tgccttactg
     1981 gtccgagagt ttcgttcccc agttgatcga gatacgcctc atgaccgatg tacaggccgc
     2041 cgaactggcg gcgttgagga ggaccgaaaa acagctggag gaacttcgac agtgcctcgc
     2101 cactttcctg gagatcggcg tggatacccc ccaggaagca aaactctctt cccactacga
     2161 atatattttc cacaggctca tcgtggcggc agcgaacaac gatatattga tcagggtcta
     2221 cgaagggctc agcgccctga tggaaaaaaa caacgacttc cttcatgaga agctgacccg
     2281 tgacacgacc tggggtaaac gcgtgaagga acatcacagg aacatctttc aggcaatcca
     2341 ggacgaagac cctgaaaaag cgaagcaatg gatgcggact caccttttcg agacgaggga
     2401 caggtacggc aagatcctgg aggaattgaa aggacttttt atataaaacc tgccggccgc
     2461 gtcgggaggc gccggcaacc ccgaaaagga acgcgatccc aggcccgtcg gctccatcgc
     2521 cggatccgcg gcgggttccc ctcatgaaac cgaggcctcc tctcggaggc ctcggcgctg
     2581 gatcgagatt acccctcccc tgaaacgccg cccctacatg aagccgctgt acctcctgcg
     2641 gcagaccgcc gaatggacgt ggttgatcat ggtaatgaag tcaaacacgg ggaggtcggt
     2701 cacttcctgg atggcagccg cataaggagg aaggtcgctg cattccagga gcacggcgcc
     2761 gaggttcggg ttctcctcct tcatcctgga ggctacgccg catacttcct tttctatgag
     2821 gtccgaatcg agggtcccct tctcgtctag tatggaggtc ctgaattcgt cctggtcctc
     2881 catgccgtag acgacgacgg gcatatcggg agtgatcccc accgactcga aatgctccga
     2941 agtgagctgt gccttgttgg ccgtaatgat gccgacggac cttcccgggg gcaggatccg
     3001 gtggacaaag ggcacctgga gcaggctgga gatgaagacc gggatgtgga ccgcctgggc
     3061 gacctgccgc tggaaaagag ccatgaatcc gcaagcccct gttatcgccc tcacaccttc
     3121 cctttcaagc tcttgggccg ccttgatgaa gagctccagg agggaggggt ccctctgatt
     3181 gatgagtttt tcgctcgaag cgccgctgac ctctttgtaa cggaccggga aatcgtaagt
     3241 tgtggcgttg cccacgttcc ccgggataca ggggtaacag gcatcgagta tgagaatgcc
     3301 tatcggtacg ccatcccacg acctggtcct gctcctcacc ttgtaacggg ccatgctgaa
     3361 ttaccccttc ctgcgtgata tgctctctcg aaagatcgct tcgtgacctc aggcagcatg
     3421 atatttcaag gcggtgccga gagcaagccg ccccgtgaag cggtccgcca aggcgcgcct
     3481 ctttcactag cctgggatcg gcgccgccgc ctatgcctgg aggcagtgtt tcgaaacaaa
     3541 aacctccgcc acggcgggcg aaccaggaaa attgttcccc tctcagctgc atcgggtgtc
     3601 caggtgcgac ggcagccagc cgacgatcac ctggctgctt tcctcgtccc agaagaaata
     3661 gatccggagg gtctccctct catccctgga cacccccttg gcaaggtgag tgtccagcag
     3721 cctgcggctg gacgttcccg gtgggtacga aacgtagtat tcgtccccct gctcgccggc
     3781 gctcttttcc ctgatggcgg ggcgatgttc caggcccagt tcccggcagc acccttcaaa
     3841 gtcggctaga ctcaacatgc cgtaacgcat gtcacggtat tccgtcgcta ggaggaggag
     3901 ggcccggtag actaactcga cacctttgta ccgagctttt ttcaaggcgt tcctcgccct
     3961 aggtagaagc tccacccttc ctgcaagatt cttttcgacc cactgaggca tggcctcgta
     4021 gtcgtgcggg atagggacct cctcatcggg atcatctacc gggaccttca tcgccgatct
     4081 aagggcctcc agggcggccg ccaaggtgtt gcccctgttg cgatgataat cagcctcgct
     4141 ggcgtagtcc gtcgccaggg cggccatctc cgcagccgat tcctcggact cgtcgatcct
     4201 ggcctgcatc gcctccctca gctcgtcaaa ctcggcgatg cacttttcgc agtccttttc
     4261 tccaaggcgc tccttcaatt cccttaccct gaggtcggcc tgcaccaacc tggcctcggc
     4321 aaggaagacc agcggcttcc actcgaactg gacccggggg ttcgccaccc gtgttttcgc
     4381 gatgacctct tcgacaaagg ccgactcgcc gaaaccgtcc ctgtagcgcc agtaatagat
     4441 gcgatctttc atggccaggg ggtgcgacct ggggctgtct ttttcctggt cgatcccggg
     4501 catataggtt cggacggctc cgtcgaagac ggaccatatc tttcctaccc tgtcggtcca
     4561 ttcgaaagag gcatccctgg caaggcaggc cacgtgggca tagccgaggg tctttcttgc
     4621 cagctcccgg cagtcgatcg tccattcggg aaactccccc ggtaatgtcc atttcgaacg
     4681 gtcgggctgg gtcgccacaa ttacggggat cgtcctctcg ggggcgaaga ccagttcgaa
     4741 aagtctccct acctcagccg aggtccggac accccagggc tcgccgtcga gggggcggac
     4801 ctgggagagg ccgacggtgt cggccaggga ttttattatc ccggggcgga tgaaggccac
     4861 gaagtcgtcc ctgtcgggaa gggaagagca ggatatcagg acgccgaaga ggatccccct
     4921 atccgtttta gtcacggaga ggtcgctcag ccaggtcctc cccggtacgg cccgtaattc
     4981 gcccattccg gcgtcggggt gggaaaaacg aaccgcccag acccccttct cggggataga
     5041 gacgcactcg agcctctggc cgggctcctc caggacaaag gactcaccct gcccggccgc
     5101 ctcaggcagg ggcgtgggga ctttttgggc gagccaccgg acggattctt cgatggtcct
     5161 gaagaagggg tcctctcctt ccttcaggag gtgacccgtg tgggccttca ggcagtaggt
     5221 ggtcgtgatc ctcggcctgc cgggttttat gaaagttctt tccggaaccg gtccatccat
     5281 gggtcatgcc tccttttttg gcctttgtca tcctgcggat gctgcccttg ccaaagaccc
     5341 gcccaagggg gggttagaaa aaacaggcct gtcgcgacga cttccaaccc ttccgaccat
     5401 tcaaggtgga gcatccgagt cagcgaaggc tgacctccca gtaccctttg atcacgaatt
     5461 tggtatggac gatcaaggtc aagcgtccct acatcttcgg ttgacgactc caaaaactcc
     5521 cacctcaaaa tatccgacgg caccctggag gtccgcgacc tccccaaatg acccacttct
     5581 tcatcccggg cgactctcaa ttgcctgttc cttcccggtg ctcttcaatg ccgttgatat
     5641 cttccaatag gagaggtgct tattcatttc attgggtgcc tattcaacag accgcccagg
     5701 ggcaggcttt agttgcgtga tcctcctgtc ccggatgatc tgccggaccc tctttcatcg
     5761 ttattttcct gaccaagata ataaatgata tgatgctttt aattcaatga agataacatc
     5821 aaaaaccgct gcgctctgca aaggtgcggg catgacgccg caggataggc cgatgccggg
     5881 caaggtttcc tgatccgggg cgatcgcttc aaaagaaaga ggacagatcc actgaactgg
     5941 ctggtctaat gatcaaacaa ggcgaggggt cacgatggtc tggtaatatg gtcaaggaag
     6001 ttttcgaaac aggaaccggt cgcaatggct cgacgctctt gatgctctcg ttaattgcga
     6061 taacctcaat ggaaagggag cggaatgaga tcatcttcta gttgggacaa acccgaaagg
     6121 gcaccgaatt tcatcgccag gaaaagatca gactggtctc tgttccgacg gggaacccat
     6181 atcccgctgg aattcctgcc tgattttgaa aaagccaacg gcaattatcg cttgaaaaga
     6241 ggcgaggcca gggaggtcat cctcctctac caagacaggt tcttccaggc gacaatttgt
     6301 aatatcgatc gcccgacgaa aagcgagacc gttcagatca ggttcgatgg caacaacgag
     6361 ttaaaagcac ttctgcggga ggaattgaaa aaaagtttta atcaccttga acaaaaaggt
     6421 ccccacaccc gtgtcccccc cgacctcgct gaatacgtgg agttctacaa aacggggata
     6481 cccttcgttt acaggatcct tttcattaaa atcggtgata gccttttcaa cctgtgatct
     6541 tgcgggtggc aggcaaagac ttgtttgcct tctatcttga aagacccggg atgtcccggc
     6601 caggggataa aggccgcgcc tttttgttcg aaccgtccgg caatttatct ggaagagtag
     6661 ctgggctgtc aagcgacacc aggaattgac ccgcttgcgc catgaaggat tgacccactc
     6721 tgcgccgtgg aaaaggtttt ccattgtggg taatgccggc ttgaaaaaca gtcaaagcca
     6781 ggtgcgcctt ggaccagccg gtgggtggct aaagcgaaga cgaagagagg gacgaccccg
     6841 gacgaaaggg aggtaaagtg aaaaacagcg caatatcaaa agtgtagacc tgacaccagg
     6901 tcttaccggt ggtactcctt gttcacgagc cagaaagcca cagagccgtc tttttccctg
     6961 taaaggaaga ctttgtaccg gtctctctcc cacaaactat ttacattcgg cgaataggtg
     7021 tcaactgggc cgagaaagct ctcaaaaaat gctttcaacc cctcaaaggc ctccctgtcc
     7081 ttgtacaggg ccgtacctct cagcatggag aaggtggagt ttatgaaacc gtacaggaaa
     7141 gcgcctttct tcatggggat cccgcccaat tctgaaactt cgaggtcccc ctgcccaagg
     7201 tagaaactta tattcaggtc gtgatcccgg gagatgaact ggatattgac tgttccgtag
     7261 gacttggccc cccacttgag gcccttgaac ccatcttcga agggatcctc cagggccggc
     7321 gggcatgact ccccatcatc cagcccctcc aggtacgccc cggcggagac caggttgctc
     7381 tcgtcgtcag ggttgtggac tatctccacc caatggcggt cactttccca tctcacgtag
     7441 ttcaactcgt ccgagcctcg agacggggtc cccagctccc tctccaggac ttcgcaccag
     7501 gtctcccatt cagcccgccc ggggcgtccc tcggccagga aaccctcaaa cctgagctgc
     7561 ctgaagagga aaagggggct ttcaatgaac ttgaggccct tccaggttct agccacgccg
     7621 tcggcgcggg ccgaggtcag ttgcccctct tcctcgtcgg atccctcgta gtccgacagg
     7681 tcttggaact ccgaaaaggg ctgaccccac cggtaatcgt cgtaactctc cggttcattg
     7741 aaggaaaaag ccggcgagac cgccaacaat gtcaacaggg tgaaaaatac cagggtcttt
     7801 ttcaaagcca tccctccctt gcccaggtta tggggcgctc tggaactcct ctttgaacag
     7861 cactattaaa agcgggatca gggaccctgt caaatgggct aaaaggcttt catgaagggc
     7921 ttcccttggg aaaaatgcct gggccagttc aggcttgcgt ccgatcccgt ccgttccacg
     7981 ccgcccaatc atgcccgggg tcgaccggcg gtgagaatag ccctcggata ggttattccg
     8041 cccgatgaag atcatcgccc cgtcacggtt aataaggggc tttgcagaca actttaacag
     8101 gggggcacgg accggatcag cgtgaccacc aggtcgcagg gctggtcctc ttcgttggag
     8161 aggcggtaac ggctattcat gaggccgtgg tcgatcgcca agacatcgcc ctggtcgagg
     8221 cgctcctcgg tttcgccgta tcggaaggtg agcctgcctg cggcgcagta gattatttcc
     8281 agttccaacc cggccccgta gttgcccttg cgggggtata ggacgctgcc ctttgacagg
     8341 gtcatggccg tggtttcccc acggcaggtt ttcctgtcca tgatgcagtt gaagtcgcgg
     8401 accttccccc ggctcctggt ctcggctccg ccgtcgaaaa ggtggggctc gaactttttc
     8461 agggtgatct cttccccgtc gtcatgggac agggcgaggt cattgtcgat ggtcatgatg
     8521 atccggtcga agccctcgaa gggggtgaag gtcgat
//
LOCUS       DAOSRG010000038         8358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_8067_length_8358_cov_10.294592, whole genome shotgun sequence.
ACCESSION   DAOSRG010000038 DAOSRG010000000
VERSION     DAOSRG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_8358_cov_10.294592"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLM30_02610"
     CDS             <1..360
                     /locus_tag="PLM30_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP protein"
                     /protein_id="HOR54094.1"
                     /translation="FQAWSFRDVNKVWIQYNDYFEQHWIVINKGAWDQLPVDIQAIMQ
                     QAANEASAMRWDQVETEDEDYREILRKDFGWEIVMLTSEELKACADKVRREVWPKMKD
                     LLGEELYTEVRLHSLAE"
     gene            549..869
                     /locus_tag="PLM30_02615"
     CDS             549..869
                     /locus_tag="PLM30_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR54095.1"
                     /translation="MEFTDTGKMESREVVKGVHGKFVHSPHMTMAWWVIEQGAALPEH
                     SHEHEQVVNVVEGTLELTAEGKKWTLGPGSVLVIPSGVPHLARGLTECRVIDAFWPAR
                     DDYK"
     gene            complement(950..4291)
                     /locus_tag="PLM30_02620"
     CDS             complement(950..4291)
                     /locus_tag="PLM30_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54096.1"
                     /translation="MMLLCPARGFLPLFLSALLAASLMASCCPAVSQEDLSSLRPGDP
                     VPEQFTFGGEPPSLEEVREAFLRTYGDDPALWPEEFRHIHEVQVAFNRRAMAHPKFKG
                     QFDKEAQAKMKAQETDPASYWARCLFDGWGFEEWFNNDHLTVQDVPRGLLDRFMIEFF
                     PLAWQDIRSIIRVEEYMLGLPDKGFVPYNQYEGKVLQQKKRDVQMYNSLVEKAAEGYA
                     AGNDIPMLLLLSERTDTNWAGDKAFEASLVKQGQKLSQKALDGLDRIDGLFRAAFYRA
                     CPRFTYSWNAEPWSEDHVVFNGNPVQEGPNTDHIGTATRIEYPYIGEKVTIPEQKVVS
                     GPALRLQWGTSPAHSPEYDRGAVVEEVTPRYLVTRKQYGETREFILSLYLDGVSGFGH
                     EVSFTPAGGVRFPQAVEEEIIEAVTGAFLEAAEECFGVPVKETAPKPAPQEEPPSRPE
                     EGREDGKHPRTVTLEPAKMKDMFVDGEHPGGTSFTLSVREGDRRVPQAEVAIRKPEVG
                     TVSSRNAEGGDEKWLLVRTDPLGEVTVTYTPPPLAELRMLGPSQWEVRIAVEDRESGA
                     GHSISFKVTRPRGLDASVDHPVFPALAGFRDTLRFRFDGRGAGDDRGEKYRVKITVRS
                     GNGSLSPDRSGSGGKSAFAMEVEADKDHVLYYRWFGPRDLAGPATESILIQVPELELE
                     ETVTFSVGADLEIHSAQQEQLELEQPGLFVPLKIYVKDAFHPDLDMAEFFRSFRLRPV
                     LDISQADFKPIDPETSASRLNLTALLDHVKGAALPRGGVPIEPETWSLAKDACSRWFL
                     VGGPAHGGPIPPNAFPGIILWDYGDYTLKIRMKVLDPQDLPAEPLERAVTMNLHVGPF
                     ATGDRRGDLILPMILTFSALFPGEDARQLSVRGRSLLQKEDLASASAMVGEYFSRRLS
                     SVSLGEEVDPQERERLEYLVYKAHGLSGAELSSATMEESLSQAKLNFLCAAAGEYAEV
                     FLSQGYDLSKLADSPSVNHGGAELEVLEMIKGFLDGYGEYGILALARENIQLLAIYDE
                     SGEVLSEYHGQVFGGGGGSRRVFFGKNSVVVPFRLGENLLINLRGKGKPVNAIKILPN
                     GINVQRLGLRPGVETISVYGDVIRP"
     gene            4460..4861
                     /locus_tag="PLM30_02625"
     CDS             4460..4861
                     /locus_tag="PLM30_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54097.1"
                     /translation="MLYFRFLALLFGTTMVFLAPVIALRGQRWIDLFSEALIPEKQPV
                     WFWAAGAFAAFLTLITWYMQITSPVTLSWVMTLFITLSLVKAYCFIFRYDQTRKVALS
                     LMDKGRTFTTGLAGILFLAGFCILCLGIFAF"
     gene            complement(4956..5120)
                     /locus_tag="PLM30_02630"
     CDS             complement(4956..5120)
                     /locus_tag="PLM30_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54098.1"
                     /translation="MKEKRVIITNLACTSDLCSLVEYDIQVLRDLIDNMEVGWVESQT
                     MDPKSCRDCS"
     gene            5314..6693
                     /locus_tag="PLM30_02635"
     CDS             5314..6693
                     /locus_tag="PLM30_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR54099.1"
                     /translation="MRLEMIPDYDVLFEGLFENSPEGVVIQGLDGVVHKANRAFCEMF
                     GYALEEVIGHDLDRLVATDESLNLEAAERTQRAILGEELVFETVRQRKDRTRFHVMAL
                     GVPIVVEGRLVAIYAMYRDITDRKKAEEALQRSEEQYRAIVQQQQEVIVRYAPDWKVT
                     FANEAYCKYYQTTPDEIIGTSMTPHMPDENRKEILGSLKKLDPGSPTQVSEELTILPS
                     GETRWQQWIDTALFDGEGNLVEYQSVGRDISDRKRMEESLRVSGERLERVLDETVRVL
                     AGTMRIRDVYTADHQEGVAQLAYAIGKEMGLSEDVLKGLWMAALIHDIGKLHIPGEIL
                     NKPTTLTALEYEIVKRHPEVGSETLKGISFPWPIAQIVSQHHERLDGSGYPRGLRGDQ
                     ILPEVRVLSVADVMEAMCSHRPFRPAYPLETALDTIHAGAGTLFDERVVQACLKAFER
                     GFRFKRAPR"
     gene            complement(6698..>8358)
                     /locus_tag="PLM30_02640"
     CDS             complement(6698..>8358)
                     /locus_tag="PLM30_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR54100.1"
                     /translation="GATTVDEYRKHIEKDAALERRFQPVLVEEPTVEDTISILRGLKE
                     RFEVHHGVRIQDNALVAAAVLSNRYISDRFLPDKAIDLVDEASALIRTEIDSLPADLD
                     AAARRVMQMEIEEAALSKETDKAGRQRLGSLKKELQDARAAADALRARYEAEKQAIGK
                     LQGLREQIESVKRDIERAERDYDLNRAAELKHGKLPDLERRLAAEEEMLVSRQGGEKL
                     LREEVTAEEISRIVSRWTGIPVSRLLEGEREKLLRLDDILHQRVIGQDEAVRLVADAV
                     LRARSGIKDPKRPVGSFIFLGPTGVGKTELARTLAEALFDSEENMVRIDMSEYMEKHS
                     VSRLVGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHHDVFNILLQILDDGRLT
                     DNRGRTVNFTNTIIIMTSNIGAADLMEGITPSGEIPEAVQKSVLRQVRASFRPEFLNR
                     VDDIVLFKPLTVGEIEQIVDLLAAGLQKRLRDLGMTLEITPAARNHVAREGYDPVYGA
                     RPLKRYMSREIETPVARLMISQALGEGSRIVVDEQEGKLAVRAS"
BASE COUNT         1494 a         2610 c         2623 g         1631 t
ORIGIN      
        1 ttccaggcct ggtccttccg ggacgtcaac aaggtctgga tccagtacaa tgactacttc
       61 gagcagcact ggatcgtcat aaacaagggg gcctgggatc agctcccggt agacatccag
      121 gcgatcatgc agcaagccgc caacgaggcc tccgccatgc gctgggacca ggtcgagacc
      181 gaggacgagg attaccggga gatcctgagg aaggatttcg gctgggagat cgtcatgctg
      241 accagcgagg aactcaaagc ctgcgccgac aaggtccgca gggaagtgtg gcccaaaatg
      301 aaggacctcc tcggcgaaga gctttacacc gaggtccgtc tccattccct ggctgaataa
      361 ggtccgggtc gcccctttta attgatccga tcccgttaaa aagccgaggg gcgggaatga
      421 ctcccgcccc tggctctttg gtgccccggt gtgcctttag gccagggcgt gacggccgtg
      481 gaaatgatcg tgggaaaccg ctatcattgt cgctgtcggt ctttaaattc agaataggag
      541 gtcacgccat ggagttcacc gatacgggca agatggagtc cagggaggtt gtgaaggggg
      601 tccacggcaa gttcgtccac tctcctcaca tgaccatggc gtggtgggtg atcgagcagg
      661 gtgccgctct gccggaacac tcccacgagc atgagcaggt ggtcaacgtc gtggagggca
      721 cgctggagtt gaccgccgag ggaaagaagt ggacccttgg gccggggtcg gtgctggtga
      781 taccttcggg ggtgccgcac ctggccaggg gtctgaccga gtgccgcgtg atcgacgcct
      841 tctggccagc ccgggatgat tataagtgag atctcaaggg catcgggtgc ataagggaag
      901 agcgccggcc aagggccggc gctcttttcg cgttccacct cacccgttgc tagggtctga
      961 tcacatcccc gtagacgctt atggtctcga cccccggcct caggccgagg cgctggacat
     1021 tgatgccatt gggaaggatc ttgatggcgt tcaccggttt ccccttgccc ctgaggttta
     1081 tcagcaggtt ctcccccaga cggaagggca ccaccacgga attcttcccg aagaaaaccc
     1141 tcctcgatcc cccgccgccg ccgaagacct ggccgtggta ctcggaaagt acttcccccg
     1201 attcgtcgta gatagccagg agctggatgt tctccctagc cagggccagg atcccgtact
     1261 cgccgtaccc gtcgaggaag cccttgatca tctccaggac ctcgagctcg gcccctccat
     1321 ggttgaccga gggggaatcc gccagcttcg agagatcgta gccctgggag aggaacacct
     1381 cggcgtactc accggcggcg gcgcacagga agttgagctt cgcctgggag agggactcct
     1441 ccatggtggc gctggacagt tcggcccctg agaggccgtg ggccttgtac accaggtact
     1501 cgagcctctc cctttcctgg gggtccacct cctcgccgag tgagaccgat gacagccgtc
     1561 ttgaaaaata ctcacccacc atggccgagg cggacgcaag gtcctccttt tgcaggaggc
     1621 tcctgccccg gacggagagc tgcctcgcgt cctcacccgg gaacagggcc gaaaaggtca
     1681 gtatcatcgg aaggatgagg tcgcctctgc ggtcgccggt ggcgaagggg ccgacatgaa
     1741 ggttcatcgt cacggcccgt tccaggggtt ccgccgggag gtcctggggg tccaggacct
     1801 tcattcttat tttcagcgtg tagtcgccgt agtcccacag gatgatcccg gggaaggcgt
     1861 tcggggggat cggtcctcca tgggcggggc caccgacgag gaaccacctg gaacaggcgt
     1921 ccttcgccag ggaccatgtc tccggctcga tggggactcc gccgcggggg agagcggccc
     1981 ccttcacgtg gtccagcagc gccgtcaggt tcaacctcga ggcggaggtt tccgggtcga
     2041 tcggtttgaa atccgcctgg ctgatgtcca gcacgggtcg gagacggaag gaccggaaaa
     2101 actcggccat gtcgagatcg gggtggaacg cgtccttgac gtagatcttg agggggacga
     2161 agaggccggg ctgttccagc tccagttgct cctgctgggc ggaatggatc tcgaggtccg
     2221 cccccacgga aaaggtgacg gtctcttcca gttccagttc aggcacctgg ataaggatgg
     2281 attccgtcgc cgggccggcg aggtccctcg gcccgaacca gcggtaatac aggacatggt
     2341 ccttgtccgc ttcgacctcc atggcgaagg cgctcttgcc ccccgagccg ctccggtcgg
     2401 gggagaggga gccgttgccg cttctgacgg tgatcttgac cctgtatttt tcgcccctgt
     2461 catcccctgc ccccctcccg tcaaagcgga aacggagggt gtcccggaac ccggcaaggg
     2521 cgggaaagac ggggtgatcc acggaggcgt ccaggcccct gggccgggtc accttgaaag
     2581 agatggaatg cccggccccg ctctccctgt cctcgacggc gatgcgcacc tcccattggc
     2641 tggggcccag catcctgagc tccgccaggg gtggcggtgt gtaagtgacc gtcacctctc
     2701 ccagggggtc agtcctgacg agaagccact tctcgtcccc cccctcggcg ttcctggaag
     2761 agacggtccc cacctcgggc ttccttatgg ccacttccgc ctgggggacc cgccggtcgc
     2821 cctcccgtac agacaacgtg aaggatgtcc ctcccgggtg ttccccgtcc acgaacatat
     2881 ccttcatctt ggccggttcc agtgtcaccg tcctcgggtg cttgccgtcc tcgcgtccct
     2941 cctcgggccg ggaggggggt tcctcctgcg gggccggctt aggggccgtc tccttcacag
     3001 ggacgccgaa gcactcctcc gcggcctcga gaaaagcccc ggtgaccgcc tcgatgatct
     3061 cttcctcgac cgcctggggg aagcgcacac ctcccgccgg cgtgaaggac acttcgtgtc
     3121 cgaagccgga gacaccgtcg aggtacaaag agaggatgaa ctccctggtc tcgccgtatt
     3181 gcttcctggt caccaggtac ctcggggtga cctcctccac cacggccccc cggtcgtact
     3241 ccggcgagtg ggcgggggat gttccccact ggagcctcag ggccggtccg gagaccacct
     3301 tctgctccgg gatggtgacc ttctcgccga tgtagggata ctcgatccgg gtagccgtac
     3361 cgatgtggtc ggtgttcgga ccttcctgga cggggttgcc gttgaaaacg acgtgatcct
     3421 ccgaccaggg ttcggcgttc caggaatagg tgaacctcgg gcaggccctg tagaaggcgg
     3481 cgcggaacaa cccgtcgatc ctgtccagcc catccagggc tttttgggag agcttttgac
     3541 cctgtttcac gagagaagcc tcgaaggcct tgtcgccggc ccagttggtg tcggtccttt
     3601 ccgacagcag gagcagcatg gggatgtcgt taccggcggc ataaccctcc gcggctttct
     3661 ccacgaggga gttgtacatc tggacgtcgc gtttcttctg ctggagcacc ttgccttcgt
     3721 actggttgta ggggacgaac cccttgtcgg gaaggccgag catgtactcc tcgacccgga
     3781 tgatgctcct gatatcctgc cacgccaggg ggaagaactc gatcatgaag cgatcgagca
     3841 aacccctggg gacatcctgc accgtcaggt ggtcgttgtt gaaccactcc tcgaagcccc
     3901 agccgtcaaa gaggcaccgc gcccagtacg aggcggggtc cgtctcctgg gccttcatct
     3961 ttgcctgggc ctccttgtcg aactgtcctt tgaatttcgg gtgggccatg gccctccggt
     4021 tgaaggccac ctggacctcg tggatatggc ggaactcctc cggccacagg gccgggtcgt
     4081 cgccgtaggt tctcaggaac gcttcccgta cttcttccag ggagggcggc tcaccgccga
     4141 aggtgaactg ctccggcacc gggtcccccg ggcggagcga tgaaaggtct tcctgcgaaa
     4201 cggcgggaca gcaggacgcc atgagcgaag ccgccaggag agcggaaagg aagagcggaa
     4261 ggaaccctct cgccgggcac aacagcatca actccccccg gcctgttcgt ccggcggggg
     4321 ataggggaac ctaaatagaa cccccgctgg aaaacaggcg agttatgtat aatgatatca
     4381 atagcaatgg actgcgggaa ttcgtcttgt gaacctgccc aacacggata caaatgacct
     4441 gggaggagat gaagagagta tgctttattt tcggtttctt gctctcctgt tcgggacgac
     4501 gatggttttc ctggcaccgg tgatcgcctt gaggggccag cgatggatcg acctgttcag
     4561 cgaggccctc atccccgaaa aacagcccgt gtggttttgg gccgccggcg cctttgccgc
     4621 cttcctgacg cttataacct ggtacatgca gatcacgtca ccggtgacgc tctcatgggt
     4681 catgaccttg ttcattaccc tttcgcttgt gaaggcctac tgcttcatct tccggtacga
     4741 ccagacccgg aaggtggccc tttcgctgat ggataagggc cgcaccttca cgacagggct
     4801 ggccggcatc ctgttcctcg cggggttctg catcctctgc ctgggaatat tcgccttctg
     4861 atgtcccaag ggggcaaagc tgaaaaaacc ggcccccccc tcgagcgggg aaccggcttt
     4921 tatctcaccc ctgtcgtaaa ggggggccgg ctttttcagc tgcagtcccg gcaggatttt
     4981 ggatccatgg tctgcgactc gacccatccc acctccatgt tgtcgatgag gtccctgagc
     5041 acctgtatgt cgtactcgac cagggagcac aggtcggaag tgcaggccag gttcgtaatg
     5101 atgactctct tctctttcat ggacagcctc ccggcgggag ggatcgtcac tggtatcccc
     5161 cgccgggtaa atgatatcac cccgggaggg gggcatcaag gccctgggcc tcccggggtg
     5221 agcgggtccc ccttcaggcg gtgtatagaa agtgtacatc atgttatact gacctttgta
     5281 taaaggatcc tcctcttcca ggaggcgaag acgatgcggc tcgagatgat ccctgattat
     5341 gacgtacttt tcgaaggtct tttcgagaac tccccggagg gggtggtcat tcagggactt
     5401 gatggggttg tgcacaaggc caacagggct ttctgcgaga tgttcggtta cgccctggag
     5461 gaagtgatcg gtcacgacct cgaccgcctg gtcgccaccg acgagagttt gaacctcgaa
     5521 gccgcggagc gcacccaaag ggctatcctg ggtgaggagt tggtgttcga gacggtcagg
     5581 cagaggaagg accgcacccg gttccacgta atggccctcg gggtgcccat cgtggtggaa
     5641 ggccggctgg tcgccatcta cgccatgtac agggatatca ccgaccgcaa gaaggccgag
     5701 gaagcgctcc agaggagcga ggaacagtac cgggccattg tccagcagca acaggaggtc
     5761 atcgtgcgct acgcccccga ctggaaggtg accttcgcca acgaggccta ctgcaagtac
     5821 taccagacca cccccgatga gatcatcggc acttcaatga cgccccacat gccggacgaa
     5881 aaccgcaaag agatcctggg gtcattgaaa aaactggacc cgggatcccc cacccaggtc
     5941 tccgaggaac tgacgatcct cccctcgggc gagactcgct ggcagcagtg gatcgacacg
     6001 gccctcttcg acggcgaggg gaaccttgtc gagtaccagt ccgttgggcg cgatatctcc
     6061 gataggaagc gcatggagga atctctcagg gtgagcgggg aaagactcga aagggtgctc
     6121 gacgaaacag tgagggtcct cgccgggacc atgaggatcc gggacgtcta cacggcggac
     6181 caccaggaag gcgtcgccca gctggcctac gcgatcggca aggagatggg gctttccgag
     6241 gacgtcctga aaggcctgtg gatggcggcc ctcatccacg acataggcaa gctccatatc
     6301 cccggggaga tcctcaataa acccacgacg ctgacggccc tggagtacga gatagtcaaa
     6361 cgccaccccg aggtcgggag cgagacgctg aagggcatct ccttcccctg gcccatcgcc
     6421 cagatcgtat cccagcacca tgaacgcctc gacggctccg ggtacccgag gggcctcagg
     6481 ggtgaccaga tactccccga ggtgcgggtc ctctccgtcg ccgatgtgat ggaggccatg
     6541 tgctcccaca ggcccttccg cccggcctac cccctcgaaa cggccctcga cacgatccat
     6601 gccggtgcag ggaccctttt cgacgaaagg gtcgtccagg cctgcctcaa ggccttcgaa
     6661 aggggcttca ggttcaaaag ggccccacgg tgacggctta cgaagcccgc accgccagct
     6721 tcccctcctg ttcgtccacc actatccgcg acccctcgcc cagtgcctgg gagatcatca
     6781 gcctggctac gggggtctcg atctcccggc tcatgtaacg tttgagcggt ctggcgccgt
     6841 agaccgggtc gtaaccctcg cgggcgacgt ggtttctggc ggccggcgtt atctcgaggg
     6901 tcatccccag gtcgcgcagc cgcttctgga ggcccgcggc cagaaggtcc acgatctgct
     6961 cgatctcccc cacggtgagg ggtttgaaga ggacgatgtc gtccacccgg ttgaggaatt
     7021 cgggcctgaa ggaggctctc acctgccgaa ggacgctctt ctggacggcc tcggggatct
     7081 cgcctgaggg ggttatcccc tccatgaggt ccgcggcgcc gatgttgctg gtcatgatga
     7141 ttatcgtgtt cgtgaagttc accgtgcggc cccggttgtc ggtgagcctg ccgtcgtcga
     7201 ggatctgcag caagatgttg aagacatcgt ggtgggcctt ctcgatctcg tcgaagagta
     7261 tcaccgagta gggtttccgc ctgacggcct cggtgagctg ccccccctcc tcgtacccca
     7321 cgtaacccgg cggcgccccg acgaggcgtg agacggagtg cttctccatg tactccgaca
     7381 tgtctatcct gaccatgttc tcctcggagt cgaaaagggc ctccgccagg gtcctggcca
     7441 gctcggtctt ccccacgccg gtgggtccca ggaatatgaa ggagcccacg ggcctcttcg
     7501 ggtcctttat cccggaacgg gcccgcagga ccgcgtcggc caccaggcgg accgcctcgt
     7561 cctgcccgat gaccctctgg tggaggatat cgtcgaggcg cagcagtttt tccctctctc
     7621 cctcgaggag cctcgacacg ggtatgcccg tccaccgcga cactatccgg gagatctcct
     7681 cggcggtgac ctcctcccgt agcagttttt cgccgccctg cctggagacg agcatctcct
     7741 cctcggcggc gagcctcctc tcgaggtcgg gcagtttgcc gtgcttcagt tcggcggccc
     7801 tgttgaggtc gtagtcccgc tcggccctct cgatatccct tttgacgctc tcgatctgtt
     7861 ccctaagccc ctggagtttg ccgatggcct gtttctccgc ctcgtaacgg gccctcaggg
     7921 cgtcggcggc ggcgcgggcg tcctggagct ccttcttcag ggatcccagc ctctgcctgc
     7981 cggccttgtc ggtctccttt gagagggccg cctcctcgat ctccatctgc atcaccctgc
     8041 gggcggcggc gtcgaggtcg gccgggaggg agtctatctc ggtgcgtatc agggccgagg
     8101 cctcgtccac gaggtcgatg gccttgtcgg ggaggaagcg gtctgagatg taccggttgg
     8161 acaggaccgc cgccgccacc agggcgttgt cctggatcct gacgccgtgg tggacctcga
     8221 aacgttcctt gagaccccgg aggatggaaa tggtgtcctc cacggtgggc tcttccacga
     8281 ggacgggctg gaagcggcgt tccagggcgg cgtctttctc gatgtgcttg cggtactcgt
     8341 cgacggtggt ggcgccga
//
LOCUS       DAOSRG010000039         7907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_8638_length_7907_cov_9.271268, whole genome shotgun sequence.
ACCESSION   DAOSRG010000039 DAOSRG010000000
VERSION     DAOSRG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8638_length_7907_cov_9.271268"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLM30_02645"
     CDS             <1..513
                     /locus_tag="PLM30_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR54101.1"
                     /translation="LSAGKIDMIAAGLSVTPERSKRAEFSRPYFFSHDAIVTRKGEAG
                     PRDAVGLKGRRVTVQAGTIQDEYLTSMGGLEVLRFQRSGEALRRVLEGGADASFMDGV
                     SVERYLADDPSLGEGLMVAFSGQITAEAMGFAVRKIDGVLLEGVNAALEALSAEGYLD
                     DLGKKWFRRS"
     gene            complement(526..1029)
                     /locus_tag="PLM30_02650"
     CDS             complement(526..1029)
                     /locus_tag="PLM30_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54102.1"
                     /translation="MYRGSWVATVLVTLSLAFSMAASTGWAAKNAGPLPDIPGWDSGD
                     LATEEYETPTEQLGCRMERLYRRQADNHSILVVWVRGPGTLWQGFPEGEVSADDGPIG
                     SGATYNTLDIDDHRAVLECHPLVGKALSVAIAPDFTLTFETTFADVGLDAFALVFLEQ
                     LGDRGER"
     gene            1125..2456
                     /gene="miaB"
                     /locus_tag="PLM30_02655"
     CDS             1125..2456
                     /gene="miaB"
                     /locus_tag="PLM30_02655"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOR54103.1"
                     /translation="MYRFCINVFGCQMNVYDADRIRTALTSRGWVEAPEEDAEVVLFV
                     TCSIRDKAEQKVWSDIGRLASPRRGPAIAVVGCMAQRVGPLLLKRFPSVRLVCGPRSL
                     GRLPTALGEVMATGKAAVLLDEDPTVLDDLPEAPLARSNPWKAFITIAYGCDNFCTYC
                     IVPHVRGRFVSRKPDDILGEVERLVSDGVREVTLVGQNVNTYGRDFHNGYVFSSLLRD
                     VALIKGVERLRYATSHPRDLTRDVVEIMAEHENICPAINLPIQSGSDRVLRVMNRGYT
                     VEEYAAIITMIRSGLPNAAITSDLIVGFPGETLEDFHESVKAVERFRFDLVHTAAFSP
                     REGTPAARMETQVEASEKRRRLKEINTAQAVITREINQDQVGKTFTVLVDGPAPKGVG
                     LLQGRTPSDKVVLFPGDPAMMGFFVKVKITGAGNWHLTGELEPARQEDLRR"
     gene            2712..>3055
                     /locus_tag="PLM30_02660"
     CDS             2712..>3055
                     /locus_tag="PLM30_02660"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR54104.1"
                     /translation="MRPGVYRLPGGTRVYQAVEQAGGLSAEADPEAFNMASPLFDGIH
                     VHVPHKGDAPGPPPAYQGGGSQGLPAGLIDINKAGAATLESLPGIGPKLAGEIIRHRE
                     ERGRFSSVEDLLS"
     assembly_gap    3056..3155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3156..4045
                     /locus_tag="PLM30_02665"
     CDS             <3156..4045
                     /locus_tag="PLM30_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOR54105.1"
                     /translation="RAGVLGLRRSLREKILMNLPPLMRGHLLAALLGGRDPDLAERHS
                     AWGTSHLLAVSGLHTGMVVLVIFLLIPAGPFRIPAASLIMWIYVLCAGASASALRAGL
                     MIQAGFVGKWLGRPSSTLNAVSVAGLFLLFWRPWFFWDLGWRLSMTAALILSCLFSGR
                     GKGSRVAAPVLLWLATAGMVTGVFGSVPVAGMIINFLAVPVFTVLLPLSFFLAVPSLL
                     GIPGGWILAEAGEGLYYAWGQFADLTASLVPWEAPYSGLLSLSRVALTAYLVFAGLGF
                     RRLHAVLFAVPIALFLALL"
     gene            4088..4858
                     /locus_tag="PLM30_02670"
     CDS             4088..4858
                     /locus_tag="PLM30_02670"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOR54106.1"
                     /translation="MLLVVDIGNTNTVLGITDGEKLVQRWRLVSERHTSDELAICLFN
                     LLSIAGVRPAEVSGAALCSVVPPLDAPWEECIRRYLSVECLKVTNRSDIGIPVLYENI
                     SEVGADRLVNAVAGIELFGSPLLVVDFGTAITVDAISLRGEYLGGAIAPGLVVSMEAL
                     FGKAAKLPKVSLDLPESVIGRNTRDSMQSGFLYGFAGMVDSLVERIRDELGEEAKAIA
                     TGGQADTIAPISRTISAVEPWLTLEGLRLVYERDRGVI"
     gene            4908..5888
                     /locus_tag="PLM30_02675"
     CDS             4908..5888
                     /locus_tag="PLM30_02675"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOR54107.1"
                     /translation="MKNPVWLAPLAGITTTSVRLFFKSMGAGLTHTEMISCAGLAHGS
                     RKTFSMLRTIEGEGPLVLQLFAPDEVTLFEGARRALSRSSFDAISINMACPMPKVTKK
                     GAGSSLLERPETAAAMVRALTPLGLPVWAKTRKPPPASPMDTLSFSGMLLEAGASHVC
                     IHGRTPSQKYGGRADRDAVVRSAKAFPGMIAASGDIFSAEDALELLDEGCSAVFVARG
                     ALKDPFLIPATLHRCGFRVDERLLAPELDTRLDLITFLGERMLQEEGEKTAVVLLKRL
                     LSGMFKGFSGASRFRKALFGTTDWRGMSEAFEECRSSPERGEPCDRREVS"
     gene            5866..>6317
                     /locus_tag="PLM30_02680"
     CDS             5866..>6317
                     /locus_tag="PLM30_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOR54108.1"
                     /translation="MTDERFPEGTEVQEDEILRQRTEKLLRLREDEGYDPFAHEKWIK
                     KHTLDFVRENFSHLTEDEKDDTEIVTAGRLMIIRRHGKATFADLADETSSMQLYFQVD
                     AMGEEEYAFFKKWVDTGDMLGVVGHPFRTKRGELSILVTRCVLLAKALR"
     assembly_gap    6318..6417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6418..7209
                     /locus_tag="PLM30_02685"
     CDS             <6418..7209
                     /locus_tag="PLM30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOR54109.1"
                     /translation="YLKRLIVGMLGRVYEIGKNFRNEGIDTMHNPEFTSMEVYWAYAD
                     YQDIMDLTEELIVASADALGSRDVVYQGTPISFHPPFRRVTMAELVREMTGFDIGEFG
                     PGDDEKARSFARAKGVDVEGGESRFVILTKLFEEFVEGNLIQPTFVLGLPTEVSPLAK
                     KNPENLDFTNRFELFINGSEVANAFSELNDPLDQRERFQDQLKKKEAGDDEAHVFDED
                     YINALEYGLPPTGGLGIGIDRLTMFLTDSRSIRDVILFPTMRPKA"
     gene            7213..>7907
                     /locus_tag="PLM30_02690"
     CDS             7213..>7907
                     /locus_tag="PLM30_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR54110.1"
                     /translation="MEAGEDTLDDKEIRERIRRLREELKHHNYLYYELDSPEIADAQY
                     DLLARELKELESRLGEAPPPDSPTVTVGGAPRGDLAKVAHGSPMLSLDNAFDREGVED
                     FIRKVSKGDTTPVLCCELKIDGLAVSLRYNDGEFVLGATRGDGHTGEVITRNLLTIPD
                     VPKKLAEKADGVLEVRGEVLIKSLDFVALNAEREEQGLPLFANPRNAAAGSLRQLDPA
                     VTASRKLSLFVYQV"
BASE COUNT         1513 a         2218 c         2417 g         1559 t
ORIGIN      
        1 ctttccgcgg ggaagatcga tatgatcgcc gccggcctta gcgttacccc ggagcggtcg
       61 aaaagggccg aattttccag gccgtacttt ttttcccacg atgccatcgt caccaggaag
      121 ggggaggctg ggccgaggga tgccgttggg ctcaagggca ggagggtcac cgtgcaggcg
      181 ggaacgatac aggatgagta cctgacatct atgggcgggc tggaggtcct caggttccag
      241 aggtccgggg aagcccttag gagagtcctg gaggggggag ccgacgcctc gttcatggac
      301 ggcgtctccg ttgaaaggta cctggccgat gatccatccc ttggggaggg gctcatggtg
      361 gccttctccg gacagatcac cgctgaagcg atgggcttcg ccgtcaggaa gatcgacggg
      421 gtactcctgg agggggtcaa tgccgccctt gaggcgctgt cagccgaggg ctacctggat
      481 gacctgggga aaaagtggtt ccgaagatcc tagggtatca ccccctcatc tttctcctcg
      541 gtcaccaagc tgttcaagga acaccagggc gaaggcatcc agtccgacgt ccgcgaaggt
      601 tgtttcgaag gtaagggtga agtcgggggc aatagcgacc gaaagggctt tccccacaag
      661 agggtggcac tccaggaccg ccctgtgatc atctatgtcc agggtgttgt acgtggcccc
      721 cgaaccaatt ggcccgtcgt cagcgctaac ctccccctcg ggaaatcctt gccagagggt
      781 gccgggtcct ctcacccaga caacgaggat cgagtggtta tcggcctgcc tcctgtaaag
      841 cctttccatc ctgcagccga gctgttccgt gggagtttca tattcctccg ttgcaaggtc
      901 gcccgagtcc catccaggga tatccgggag gggaccggcg tttttcgcgg cccaccccgt
      961 cgaggccgcc atgctgaagg cgagagacag tgtcactagg accgtcgcga cccagcttcc
     1021 tcgatacatg atccgaaccc ctttcccgtt catgagcgcc aaggtggtat catttcgtgg
     1081 tgaagagctc cctcatagat attattgtac atggaggccg cgacttgtat cgtttctgca
     1141 tcaacgtttt cgggtgtcag atgaatgtct acgatgcgga caggatcaga acggccctga
     1201 cgtcacgagg ctgggtggag gcgcccgagg aagatgccga agttgtcctc ttcgtcacct
     1261 gcagcatccg cgacaaggcc gagcagaagg tctggagcga cattggaagg ctggcctccc
     1321 cccggcgtgg tcctgccatc gccgtggtgg gatgcatggc ccagagggtg ggtccccttt
     1381 tgctcaaacg cttcccctcg gtgaggcttg tatgcgggcc ccggagcctg gggaggctcc
     1441 cgaccgcgct gggggaggtc atggctaccg gcaaggccgc ggtactcctc gatgaggacc
     1501 cgaccgtcct cgatgacctt cccgaggcgc ccctggcccg ttccaacccg tggaaagcct
     1561 tcatcacgat cgcctatggc tgtgataact tttgcaccta ttgtattgtg ccccacgtta
     1621 gggggcgatt cgtctcaaga aagcccgatg atatcttggg tgaggtggaa cgcctcgtat
     1681 cggacggggt cagggaggta acccttgtcg gccagaacgt caatacctac ggtagggatt
     1741 tccacaacgg ttatgttttt tcaagccttc tcagggatgt ggccctgatc aagggagtgg
     1801 aaaggttaag gtatgccacg tcccacccga gggacctgac ccgggacgta gtggagatca
     1861 tggcggagca tgaaaatatc tgtcccgcca tcaacctccc catacagtcg gggagcgaca
     1921 gggtactgcg ggtcatgaac agaggctaca ccgtcgaaga gtacgccgcg ataatcacta
     1981 tgataaggtc aggcctcccc aatgcggcca ttaccagcga tctcattgtg ggttttccgg
     2041 gggagaccct cgaggacttt catgagtcgg taaaggctgt tgaacgattc cggttcgacc
     2101 tggtccatac cgcggccttc tcaccccggg aggggacgcc ggcggctcgt atggaaaccc
     2161 aggtggaagc gtcggagaaa cggcgaaggc tgaaggagat caacaccgcc caggctgtga
     2221 tcacccggga gataaaccag gaccaggtcg ggaagacctt caccgtcctc gtcgatggcc
     2281 cggctcctaa aggggtaggg cttttacagg gcaggacgcc ttccgacaag gtggtcctct
     2341 tccccggcga ccccgccatg atgggttttt tcgtcaaggt gaagatcacc ggtgccggga
     2401 attggcacct gaccggtgaa ctggaaccgg ctcgacagga agacctccgg aggtgatgtg
     2461 agcccatgga gatccccagc accaggagag gtttgttcct tctggcggga ggggtcgcgt
     2521 gcctgatcct ggcggggttg ctggtttcat tattctcggg ccgatgggac agtaagccct
     2581 cgggccagat ggcaggacct gccccccttt ccacgactga tgcctctccc ccggtgaaca
     2641 cacccaggcc aggtcaaccc ggggaagatc ccttggaaga ccggtgggtt atttatatca
     2701 cgggagccgt tatgaggcct ggcgtctaca ggctccccgg cgggaccagg gtttaccagg
     2761 cggtcgagca ggccgggggc ctaagcgcgg aagccgatcc ggaggccttc aacatggcat
     2821 cccccctttt tgacgggatc catgtccatg tcccacataa aggcgatgct ccgggtcctc
     2881 cccccgccta ccaaggcggg ggttcccagg gcctccccgc cggcctcatc gatattaaca
     2941 aggccggcgc cgccaccctg gaatccctcc ccggcatcgg gcccaagctc gccggggaga
     3001 tcatcaggca cagagaggag cggggaagat tttccagcgt ggaggatctc ctttcnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccgg gccggggtcc tgggcctcag
     3181 gaggtccttg agggaaaaga tcctgatgaa cttgcctccg ctgatgaggg gtcaccttct
     3241 tgcggccctg ctcggcggga gagaccccga cctggcggag agacactccg cctggggcac
     3301 ctctcacctt ctggcggtct cgggtcttca tacgggcatg gttgtcctgg tcatcttcct
     3361 cctcattccg gcaggtcctt tcaggatacc ggcggcgtct cttatcatgt ggatctatgt
     3421 cctttgcgcg ggggcctcgg ccagcgcctt acgggccggg ctaatgatcc aggccggttt
     3481 cgttggaaaa tggctgggaa gaccttcctc gaccctgaac gccgtttccg tggcggggtt
     3541 gttcctgctc ttctggcgcc catggttctt ctgggacctg gggtggaggc tgtcaatgac
     3601 ggcggcgctg atcctttcct gccttttcag cgggagagga aaagggagcc gggtggcggc
     3661 gccggtcctg ctttggttgg ccaccgccgg aatggtgacg ggggttttcg gctcagtgcc
     3721 ggtagcgggc atgatcatca atttcctggc tgtcccggta ttcactgtcc ttttaccgct
     3781 gtcctttttc cttgcggtcc cgtcactgtt gggtataccc ggaggctgga tcctggcgga
     3841 agcaggggag ggcctttatt acgcctgggg gcagttcgcg gacctgaccg cttcattggt
     3901 gccctgggaa gccccctatt ccggcctgct ctccctttcc agggtagccc tgacggctta
     3961 cctggtcttc gccggtctgg gttttcgcag gcttcacgcg gtccttttcg cggtccctat
     4021 tgccttattc ctggctttgc tctagaaaag ggccaagtca tgcgatactt cttccggagg
     4081 tgatgccatg ctcctggtcg tcgatatcgg aaacaccaac accgtcctcg ggattaccga
     4141 cggggaaaaa cttgtccagc gctggaggct cgtctccgag aggcatacct ccgatgagct
     4201 ggcgatctgc cttttcaacc tgctgtccat cgccggggtt aggccggcgg aagtatccgg
     4261 ggccgccctt tgcagcgtgg taccccccct cgacgccccc tgggaagagt gcatccggcg
     4321 ctacctctcg gtggaatgcc tcaaggtcac caaccgttcg gatatcggca tacccgtttt
     4381 atacgagaac atctccgaag tgggggccga ccgcctggtc aacgccgtcg ccgggataga
     4441 actattcggg tcccccctcc tggtcgtcga cttcgggacg gccataacgg tcgatgccat
     4501 ttcgctccgc ggcgaatatc tcgggggggc catagcgccg ggcctggtgg ttagcatgga
     4561 ggccctgttc gggaaagcgg cgaagttgcc caaggtgtcc cttgacctgc ccgaaagcgt
     4621 cattgggagg aacacgaggg attccatgca gtccggattc ctttacggct tcgcggggat
     4681 ggtggattcc ctggtggaga ggattagaga tgaactgggg gaagaagcca aggccattgc
     4741 caccggaggc caggcagata caatagcgcc catatcccga acgatcagcg cggtcgaacc
     4801 atggctgacc ctagagggcc tcaggctggt ctacgagagg gataggggag tgatctgaga
     4861 tctccttcca tgccggatct ttcccatccc gggtgggccg ggtagatgtg aaaaaccctg
     4921 tctggctggc gcccctggcg ggcatcacga ccacgtcggt caggctcttt ttcaaaagta
     4981 tgggcgccgg cctgacccac accgagatga tcagctgtgc cgggctcgcc catggaagtc
     5041 gaaagacctt ctccatgctc agaacgatcg aaggggaggg tcccctggta ctccagctct
     5101 tcgcgccgga cgaggtaact ctatttgaag gcgcccggcg agctttgagt cgctcctcct
     5161 ttgatgccat aagcatcaac atggcctgcc ccatgcctaa ggtgacaaaa aaaggtgcgg
     5221 gttcaagcct cctggaacgg cccgagaccg cggccgccat ggtcagggcc ttgacgcccc
     5281 tagggcttcc cgtgtgggcg aagaccagga agccaccccc cgcctccccg atggataccc
     5341 tttctttttc ggggatgctg ttggaggcgg gtgcttccca tgtctgtatc cacggtcgaa
     5401 cgccgtcaca gaagtacggg ggccgggcgg atagagacgc tgtcgtccgc tccgccaagg
     5461 ccttccccgg catgatagcc gcgagcggtg atatcttttc agcggaggac gcccttgaat
     5521 tgttggatga aggctgttct gccgttttcg tcgccagggg agccctgaag gaccccttcc
     5581 tgataccggc gaccctccat cgttgcggtt tccgtgtgga cgagaggttg ttggctcctg
     5641 agctcgatac caggttggac ctgattacct tcctggggga aaggatgctc caggaagaag
     5701 gggaaaagac ggccgttgtc ttgctcaagc gtttactctc gggtatgttt aaagggttta
     5761 gcggagcttc gcggttcagg aaggccctat tcgggacaac tgactggcga gggatgagcg
     5821 aggccttcga ggagtgtaga tcatcaccag aaaggggcga gccttgtgac agacgagagg
     5881 tttcctgaag gtacagaagt acaggaggat gagatcctca ggcagcgcac ggaaaaattg
     5941 ctaagactcc gggaggacga gggatatgat cccttcgccc atgagaagtg gataaaaaaa
     6001 cacaccctcg atttcgtccg tgaaaacttc agccacctga ccgaggacga gaaggacgat
     6061 acggagatcg tgaccgccgg ccgcctcatg atcatcagga ggcacggcaa ggccactttc
     6121 gccgatctcg ctgacgagac ctcgagcatg cagctttact tccaggtgga cgccatgggc
     6181 gaggaagaat acgccttctt caagaaatgg gtcgatacgg gagacatgct gggggtggtg
     6241 gggcatccct tcaggacgaa gaggggcgaa ctgtccatcc tcgtcacccg gtgcgtcctc
     6301 ctggccaagg ccctccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac
     6421 ctcaagagac tcatcgtagg catgctgggg cgggtctacg agataggcaa gaacttccgc
     6481 aacgagggga tagatacaat gcataacccc gagttcacct cgatggaagt ttattgggcc
     6541 tacgccgact accaggacat aatggacctg acggaggaac tgatcgtcgc atcggccgat
     6601 gccctcggtt caagggacgt ggtctaccag ggcacgccca taagcttcca ccctcccttc
     6661 agacgggtca ccatggctga actggtgagg gagatgacgg gcttcgacat cggcgagttc
     6721 ggcccgggtg acgatgagaa agcccgaagc tttgccaggg cgaaaggggt cgacgtcgag
     6781 gggggcgaaa gcaggttcgt gattctgacg aaactcttcg aggagttcgt cgaggggaac
     6841 ctcatccagc ccaccttcgt cctggggctt cccacggagg tttctcccct cgccaagaaa
     6901 aacccggaga atctcgattt cacgaaccgg tttgagcttt ttatcaatgg ttccgaggtg
     6961 gccaacgcct tcagtgaact aaatgacccc ctcgaccaga gagaacgctt ccaggatcaa
     7021 ttaaaaaaga aggaagccgg cgatgacgag gcccatgttt tcgatgagga ttacatcaac
     7081 gccctggaat acggccttcc gcccacaggg ggactcggga tcggcatcga caggctcacc
     7141 atgttcctca ccgattcccg ttccatcagg gacgtgatcc tgttcccgac gatgaggccc
     7201 aaggcttgag ggttggaagc gggggaagat actttggacg acaaggagat aagggaaagg
     7261 atccgccggc tgagggagga attgaagcac cacaattacc tctactatga gctggactct
     7321 cccgagatag ccgacgcgca gtacgacctc ctggcccggg aattgaagga actggaatcc
     7381 cggctgggtg aggctccccc gcctgattct cccacggtaa ctgtgggcgg tgcgcccagg
     7441 ggcgaccttg ccaaggtggc ccatggatcc cctatgctca gcctggacaa cgccttcgac
     7501 agggagggcg tggaggattt catcaggaag gtatcgaaag gggataccac cccggtcctt
     7561 tgctgcgaac tcaagatcga cggcctggct gtttctctcc gctacaacga cggggagttc
     7621 gtcctcggcg cgacccgggg ggacgggcac accggggaag tgataacccg gaacctgttg
     7681 acgatccccg acgtccccaa aaaattggcg gaaaaggccg acggggtgct ggaggtccgg
     7741 ggcgaggtcc tgatcaagag tctcgatttc gtggccctga acgctgaaag ggaagagcag
     7801 ggtctgcccc ttttcgctaa tccccgcaac gcggcggcgg gcagcctgag gcagctggac
     7861 ccggccgtca cggcgtcaag gaaactcagc cttttcgttt accaggt
//
LOCUS       DAOSRG010000040         7767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_8836_length_7767_cov_7.812370, whole genome shotgun sequence.
ACCESSION   DAOSRG010000040 DAOSRG010000000
VERSION     DAOSRG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8836_length_7767_cov_7.812370"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..333
                     /locus_tag="PLM30_02695"
     CDS             112..333
                     /locus_tag="PLM30_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54111.1"
                     /translation="MRLVVLLLSLGTLVLYGRAIAALWSFMVGHPRPGMLFLGLFGGT
                     VTAVSAVLLWKNRLRELDWVKEDEEGQQH"
     gene            complement(346..858)
                     /locus_tag="PLM30_02700"
     CDS             complement(346..858)
                     /locus_tag="PLM30_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOR54112.1"
                     /translation="MTDLERLLAGREERASLQAHVLLRSEVIAQVSLNIPGLPKRMAG
                     DREVVFRVIGLIRRKIQALGGGPALLFFLDNGAGYAGILGAKGVDPVAVKRIAMEVED
                     LPWGHVVDVDVLTRTGALHRAGLTGIERKCFLCGRPAKDCARTQRHEYSQLREACRRM
                     LAAALEELRK"
     gene            complement(855..2213)
                     /locus_tag="PLM30_02705"
     CDS             complement(855..2213)
                     /locus_tag="PLM30_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOR54113.1"
                     /translation="MIEIPLDPAGRAPLYRQIATHLMKMVQNGTLAPGDRLPGSRDLS
                     SSLGLSRNTVVLAYDLLEDEGFIEQRGRRGAFISWKGLHDSGDIEDDGPRWDMASGLP
                     SFDLVPSGRLAALARDALLSSGPGALYDTPPEGLMTLRNTLVKHAATRGIPAKRDEAV
                     VTSGARHALSVYIHAMALKGVRRLWIEELTYPEVRLMAQGAGLEIATVPVEPEAFQAS
                     LAKIGPGELLYLIPSFHNPTGRTLSPEQRKNVLEAASRIGFWVIEDDAYGELRFGESS
                     VPALKSIDQESRVLYLGSFSQLLFPGMRIGYVLMPEETRKEFLSVLERTSGSTSSLVQ
                     HLVNAFIEDRSLEVSLDLARNSMASRMRTLCNCLGASLPGLPFETPQGGIYLWLRTPG
                     RSGTLAAGTAKKVGVGVVPGRLFSYAGEDIEAVRLSVSRLSTVMIQEAVKRFASAWEK
                     QS"
     gene            complement(2329..3390)
                     /gene="citC"
                     /locus_tag="PLM30_02710"
     CDS             complement(2329..3390)
                     /gene="citC"
                     /locus_tag="PLM30_02710"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HOR54114.1"
                     /translation="MFDVSIRVVERPSQEEKKLLESFLERFGLAFEGCPDVAVFLEDE
                     GGRLVGTGSLSGNVIKMMAVDPGWQEYGLSGTIVSRLVEWGRARGMTHFFVFTKPDTA
                     DTFARFGFREIARYEPHAALLEMGSPGIDQFREYLASCRREAGTAGRTGSIVVNCNPF
                     TLGHQFLIEKAASLSDHLYVIVVEADLSSFPFRDRFELVRCGTSHLGNVTVVKSGEYA
                     VSPATFPSYFLKDASMMDIASIQARLDVTLFANLFAPGLGINVRFVGTEPYCPVTNAY
                     NEAMKEILPPRGIELREIRRLETDEGLVVSASTVRSLLKSEDWEALRKMVPNCTWDYL
                     HSEKGKKVIDHLRGSRGRH"
     gene            complement(3433..4908)
                     /locus_tag="PLM30_02715"
     CDS             complement(3433..4908)
                     /locus_tag="PLM30_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR54115.1"
                     /translation="MNVRLEEKILSQCNRSNGERAVWWRGAWWSRKVLADLAAQYETR
                     LRESGFKEGNRVAVLMPNCPAFLALMIAVWKAGGTMTPLNLQAGLAPTMETLVHSDVS
                     AVFVPQGMDQITQGLAASGLPAVAVALDGPLPSFQSRTCERSDPEIAVLFYTSGTTGS
                     PKGVPITHSNLYDNVTKSIFHFKDLESDDIFINVLPNFHTLGFTTSGLLPLLGGYPQA
                     IMPTFMPPESAIECIKAAEVTVMIAVPTMVALMLGAIARGVMPPSSLRLLVTGGDRFP
                     ARLDARTKQLLGVSVLEGYGLTETSPVVSINESVARRKLGTVGTVLEGYDVEVRDTEG
                     ELLPLGAEGTLWVKGPSVFKGYFKAPELTREKIKDDWFNTGDVVKIDEEGYLTILDRV
                     TDIIIVGGFNVYPTEIEGVLQQMPGVRECAVVGVEHPVSGEIVKAYITPSGEPKVTAR
                     DVISFSKEHLAHYKIPRIVEFIDEMPRSSIGKILKRELRKN"
     gene            5073..6377
                     /gene="asnS"
                     /locus_tag="PLM30_02720"
     CDS             5073..6377
                     /gene="asnS"
                     /locus_tag="PLM30_02720"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOR54116.1"
                     /translation="MAVPWVYIGDLPAHEGEEVQLKCWMYNKRSSGKIHFLQLRDGTG
                     FIQGVVVKGEVPEEQFLSTRNLWLEASLEVTGKVRRDERAPSGFELNVTGVKILQNPT
                     DEYPIGKKDHGIDFLLDNRHLWLRSASQRAVMRIRERVIWSAREYLHDNGFLLVDSPI
                     LTGAIGEGASSLFEVPYFDLGKAYLAQTGQLYAEAAAAAFGKVYTFGPTFRAEKSKTR
                     RHLTEFWMIEPEVAFYDHVDNMTLQENLVSYIVEKVLEHCRAELALIGRDTSPLELVK
                     PPFHHISYDDAVIMLQKLGCPMQYGADFGGEDETILTRQFDRPLFVECYPKKVKAFYM
                     KQHPEREDLVLCDDLLAPEGYGEIIGGSQREDSLDLLLARIREEKLPEDSYDWYLDLR
                     KYGTFVHSGFGMGIERVVAWVCGLPHIRETIPFPRTIYRLNP"
     gene            complement(6454..7725)
                     /locus_tag="PLM30_02725"
     CDS             complement(6454..7725)
                     /locus_tag="PLM30_02725"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOR54117.1"
                     /translation="MGKRTSENVLLDIALKNFYGAAEELGLEDSLVEILGNSERKLSV
                     SVPVVMDDGTTKVFNGYRIQHSTAIGPAKGGTRFHPDVNLDECEALGMLMTWKCSLAG
                     IPYGGGKGGIECDPLQMSKGEKERLSRTYAARIEPLIGTWIDAPGPDVNTGGQEMIWF
                     MDTISKIRGKVEPALFTGKPVGLWGSEGRVEATGLGVATCALEFLKAIKKDPKGTTFV
                     IQGFGNVGTHAALTLINAGATVVGISDITGAYYCPKGIDIKKAQQHASAHPKKLLEGF
                     EQPGLEKIPAEELLLLEADILIPAALEGVVNKDNADKVKVKYIVEAANGPITPDADAI
                     LDAKGVLIVPDFLANSGGVIGSYFEWAQNLGGFFWTREEYNSRLLHIMKGNFKRVWDF
                     SQERHVKMRRAAFMAAIARVAEAARLRGIFL"
BASE COUNT         1560 a         2357 c         2280 g         1570 t
ORIGIN      
        1 ctcgaaggcg taaaaagtat tacatgaata catgcatact atcacaattt ttacggaccg
       61 acaatttttc aggttataat cagcttcgac ccggcgatag gagtgttgcc catgcggctg
      121 gtcgtcctct tgctgtccct tggaacgttg gtcctttatg ggagggctat cgcggccctt
      181 tggagtttca tggtcggcca ccccaggccg ggcatgttat tcctcgggct cttcgggggg
      241 actgtcaccg ccgtctcggc ggtgcttctt tggaaaaacc ggttaaggga actggactgg
      301 gtaaaggagg atgaggaggg ccaacaacat tgaaccgccc tccaattatt tccggagttc
      361 ttccagggcc gcggccagca tccgccgaca agcttcgcga agctgggagt attcatgccg
      421 ctgggtcctg gcgcaatcct tggccggtcg cccgcagagg aaacacttcc tctcgatccc
      481 ggtcaggccg gcccggtgga gggctcccgt tcgggtaagg acatccacgt cgaccacgtg
      541 gccccagggc aggtcctcga cctccatggc gatccttttc accgccaccg ggtcgacacc
      601 cttcgcgccc aggatcccgg cataaccggc cccgttatcg aggaaaaaaa ggagggcagg
      661 cccgccgccc aaggcttgaa ttttccggcg tatcaaccca atgacgcgga agaccacttc
      721 ccggtcgcct gccatccttt tggggagtcc cggtatattc agggagacct gggcgatcac
      781 ttccgacctc aggaggacat gggcctggag ggaggccctt tcctccctcc cggcgagaag
      841 ccgctccagg tccgtcatga ttgcttttcc caggctgagg cgaatcgctt gaccgcttcc
      901 tggatcatca ccgtcgacaa ccggctgacc gacagcctga cggcctcgat gtcctcaccc
      961 gcgtaactga acaaacggcc cggtactacc ccaacaccca cttttttcgc cgtcccggcg
     1021 gcaagggtcc ccgaccttcc cggcgttctc agccagaggt agatccctcc ctggggtgtc
     1081 tcgaagggta gccctggcaa cgaagctccg aggcagttgc aaagggtcct catccttgaa
     1141 gccatgctat tcctggccag atccagggaa acctcaaggc tgcggtcttc aatgaaggcg
     1201 ttcaccaggt gctgcaccag cgaggatgtc gaaccggagg tcctctccag gaccgacagg
     1261 aactccttcc tggtctcctc gggcataagg acatatccga tgcgcatgcc ggggaaaagg
     1321 agctgactga aggaacccag gtacagcacc cggctctcct ggtcgatgga cttgagagcc
     1381 ggcacgctgg attcaccgaa ccggagttcc ccgtaagcgt cgtcttcgat cacccagaaa
     1441 cctatccttg aagccgcctc caggacattc tttctctgct cgggcgaaag ggttctgccc
     1501 gtgggattat ggaaactggg gatcaggtag agcaattccc cggggcctat ctttgcaagg
     1561 gacgcctgga acgcttcggg ttcaacaggg acggtcgcga tctccaggcc ggctccctgg
     1621 gccatgagcc tgacctcggg gtaggtcaac tcctcgatcc agagcctcct cacccccttc
     1681 aaagccatgg cgtgaatata gacagagagg gcatggcgag ccccgctggt gacgacagcc
     1741 tcgtcccttt tcgcggggat acctctcgtg gccgcgtgtt tgaccagggt gttccgcagg
     1801 gtcatgagac cttcgggcgg ggtgtcgtag agagctccgg ggccgctgga caggagggca
     1861 tccctggcca gggcggccag gcgtcccgag gggaccaggt cgaaggaagg caggcccgaa
     1921 gccatgtccc atctcgggcc gtcatcttca atatcaccgc tatcgtgaag ccctttccaa
     1981 gagatgaagg ccccccggcg ccctctctgt tcgataaaac cttcgtcctc gagaaggtcg
     2041 taggcgagta ccacggtgtt cctgctcaaa ccgaggcttg acgacaggtc gcggctgccg
     2101 ggcaagcggt ccccgggggc gagggtgccg ttctggacca tcttcatgag gtgcgtggcg
     2161 atctggcggt agagcggggc ccgcccagcc gggtcaaggg ggatctctat caagggctca
     2221 tcccctttct ccctatcact gggtcaatga tacaccagcg gctcaacgct tacgggggct
     2281 aggcggctgc tgtccctctc ctgcgcgggg cgggggcgat cgacagcgtc agtgcctccc
     2341 tcgggacccg cggagatgat cgatgacctt tttgcctttt tcagaatgga ggtaatccca
     2401 ggtgcagttc gggaccattt tcctgagggc ctcccagtct tcacttttca gcaaggaccg
     2461 aaccgttgag gccgacacca ccaacccctc gtcggtctcc aaccggcgga tctcccggag
     2521 ctcgatcccc cgaggtggaa ggatctcctt catggcctca ttgtaggcgt ttgtaacagg
     2581 gcagtagggt tcggtcccga cgaaacgtac gttgatcccc agccccgggg cgaaaaggtt
     2641 cgcgaaaaga gttacgtcca gtcgagcctg gatcgaagcg atgtccatca tcgaggcgtc
     2701 cttgaggaaa taggagggga aggtggccgg ggagacggcg tattctccgc ttttgacgac
     2761 ggtgacgttt ccaaggtggg acgtcccgca ccggacgagt tcaaaccggt ccctgaaggg
     2821 gaaggatgag aggtcagcct cgacgacgat gacatagagg tggtccgaaa gggatgcagc
     2881 cttctcgatc aggaactggt gccccagggt aaaggggttg cagttgacca cgatcgagcc
     2941 ggtcctcccg gcggttccgg cttctcgccg gcatgaggcc aggtactccc tgaactggtc
     3001 gatccccggg ctgcccatct cgagcagcgc cgcgtggggt tcgtacctgg ctatttctct
     3061 gaagccgaag cgagcgaagg tatcggcggt atcaggtttg gtgaagacaa aaaagtgggt
     3121 catgccgcgg gcacgccccc actccaccag cctggacact atggtcccgg aaaggccata
     3181 ttcctgccat ccgggatcga cggccatcat cttgatcacg ttcccgctca ggctgcccgt
     3241 tccgacgagc ctgccgccct catcctcgag gaagacagcg acatcgggac acccttcgaa
     3301 ggccagcccg aaccgttcga gaaaactttc cagcagtttt ttttcttctt gggagggtct
     3361 ttcaacgacc cttatcgaaa catcgaacaa taccgatctc ccccgaaggc gcgaaaaccc
     3421 agggcttcag tatcagtttt tgcggagttc ccttttgaga atcttgccta tgctggacct
     3481 cggcatctcg tcgatgaact ccacgatcct cgggatcttg taatgggcca agtgctcctt
     3541 cgagaaggag atgacatccc gggctgttac cttaggttcc ccggagggcg tgatgtaagc
     3601 cttcactatc tcgccgctga cagggtgttc cacccccaca acggcgcatt ccctgacacc
     3661 cggcatctgc tgcaggacgc cctcgatctc ggtggggtat acgttgaaac cacccactat
     3721 aattatatcg gtcaccctgt ccaggatggt caggtacccc tcctcatcta tcttgaccac
     3781 atccccggtg ttgaaccagt cgtctttgat cttttccctg gtcaactccg gcgctttgaa
     3841 atagcctttg aaaacggagg ggcccttcac ccagagggtg ccttccgcgc caagggggag
     3901 caactcgccc tcggtgtccc ttacctcgac atcgtaaccc tccaggacgg tccctacggt
     3961 gcccagtttc ctcctagcca cgctctcgtt gatggagacc accggcgatg tctcggtaag
     4021 gccataacct tcaagaactg aaaccccaag cagctgcttc gtcctcgcat ccagcctcgc
     4081 ggggaaccgg tcgcctccgg tcaccaggag tctcagcgag gagggtggca tcacacccct
     4141 ggcaatggcg ccgagcatga gggcgaccat ggtcggtacg gcgatcatca ccgtgacctc
     4201 ggccgccttt atgcactcga tggcactctc gggaggcatg aaggtgggca tgatggcctg
     4261 ggggtacccc ccgagcagag gcaggagccc gctggtggtg aaacccaggg tgtggaagtt
     4321 gggaagtacg ttgatgaaga tatcgtcgct ttccaggtcc ttaaaatgga agattgactt
     4381 tgtcacgttg tcgtataggt tggaatgggt tatgggcacc cccttgggtg accccgttgt
     4441 gccggaggta tagaagagaa cggctatttc ggggtccgat cgttcgcagg tccgggactg
     4501 aaaggaaggc agggggccgt ccaaggccac cgcaacggcc gggaggccgg aagcggcaag
     4561 tccctgggtg atctggtcca taccctgggg gacgaaaacg gcagatacat cggagtgaac
     4621 gagggtctcc atcgtcggcg ccaacccggc ctgcaggttc agcggggtca tggtcccccc
     4681 ggctttccag acggcgatca tcaaggcaag aaaagccggg cagttcggca tcaacaccgc
     4741 taccctgttg ccctctttaa acccgctctc cctcaacctc gtctcgtact gggcggcgag
     4801 atcggccagg actttacggg accaccaagc tccccgccac cagacggcgc gctcgccgtt
     4861 ggacctgttg cactgggaaa ggatcttttc ctcgagcctc acgttcacgc cattgtccct
     4921 ccgagaaaag aaatgtatgg gacctcaccg gcaccatccc gttgcgcaag accatcgtct
     4981 tcccgccgca ggtcccccaa aaacggaata tatgctagtg tatcatactg tcatctattc
     5041 cgaacacgcg aggaaatgga ggaggtcttc ccatggcggt tccctgggtc tacatcggcg
     5101 atcttcccgc tcatgagggc gaggaggtac agctcaaatg ctggatgtac aacaagcgga
     5161 gttccggcaa gatccacttc ctgcagctcc gcgacggcac gggattcatc cagggggtgg
     5221 ttgtcaaggg cgaagtaccg gaggaacagt tcctctcgac ccgcaacctg tggctcgagg
     5281 cttccctcga ggtgacgggc aaggtgcgca gggatgaaag agccccctcc ggtttcgagc
     5341 tgaacgtcac cggggtgaag atcctgcaaa accccaccga tgaatacccc atcggaaaga
     5401 aggatcacgg gatagacttc ctcctggaca accgtcacct gtggctgcgg agcgccagtc
     5461 aacgagctgt gatgaggatc cgggaaaggg tgatctggtc ggcgagggag tacctccatg
     5521 acaacggctt ccttctcgtc gacagcccta tcctgaccgg ggccatcggc gagggcgcat
     5581 cgagcctttt cgaagtaccc tatttcgacc tggggaaagc ctacctggcc cagacgggtc
     5641 agctttacgc cgaggcggcg gcagcagcct tcggcaaggt ttataccttc ggccccacct
     5701 tccgggccga gaaatccaag accaggaggc atctcaccga gttctggatg atagagcccg
     5761 aggtggcctt ttacgatcac gtcgacaaca tgaccctcca ggagaacctc gtctcttata
     5821 tcgtggaaaa ggtcctggaa cattgccggg cggagctggc cctgataggg agggacacgt
     5881 cccctctcga gctggtaaaa ccgccctttc accacatcag ctacgacgac gccgtcatta
     5941 tgctccaaaa gctcggttgc ccgatgcagt acggtgctga cttcggaggc gaggatgaaa
     6001 ccattctcac ccggcagttc gacagacccc tcttcgtaga gtgctaccca aaaaaggtca
     6061 aggctttcta catgaaacag caccccgaaa gggaggacct ggtcctctgc gacgacctcc
     6121 tggcaccgga gggctacggt gagatcatag gcggctccca gagggaggat tccctggacc
     6181 ttctgctcgc acgcataagg gaagagaagc tccccgagga ttcctacgac tggtacctgg
     6241 acctgaggaa gtacggcacc ttcgtccaca gcggtttcgg catgggtatc gaaagggtcg
     6301 tagcatgggt atgcggcctt ccccacataa gggagaccat accattcccg aggaccatat
     6361 atcgtttgaa cccgtgaagg tgaaaaagcc ggggccgcct taaaaggcgg ccccggccgg
     6421 tcattgacga ttcgacggct aaaaggacgt cgactagagg aatatcccgc ggagccttgc
     6481 cgcttcggca acccgtgcga tggcggccat gaaggcggca cggcgcatct tcacgtgacg
     6541 ttcctggctg aagtcccaga cccgcttgaa gttacccttc atgatgtgga gaagcctcga
     6601 gttgtactcc tcccgggtcc agaaaaatcc gccgaggttc tgcgcccact cgaagtagga
     6661 ccctatgacc ccgccggaat tggccaggaa gtcgggaacg atcagtactc ccttggcatc
     6721 gaggatcgcg tcggcatcag gggtgatggg gccgttggcg gcttcgacga tgtacttgac
     6781 cttgaccttg tcggcgttgt ccttgttgac cacgccctcg agggccgcgg ggatgaggat
     6841 gtcggcttcg agcaagagga gttcttcagc ggggatcttt tcgagtccag gctgctcgaa
     6901 accctcgaga agctttttgg ggtgggcgga ggcatgctgc tgggctttct tgatgtcaat
     6961 acccttgggg caatagtaag ctccggtgat atcgctgatg ccgaccaccg tcgcgccggc
     7021 gttgataagc gtcagggcgg cgtgggttcc gacgttgcca aacccctgga tgacgaaggt
     7081 cgtacccttg gggtccttct tgatggcctt gaggaactca agagcgcagg tggcaacacc
     7141 caggccggtc gcctcgacgc ggccctcgga accccacagt cccaccggtt tcccggtgaa
     7201 gagggcgggt tcgaccttgc cccttatctt gctgatcgtg tccatgaacc atatcatctc
     7261 ctggccgccg gtgttcacat ccggcccggg agcgtcgatc caggttccga tcagcggctc
     7321 tatcctggcg gcgtaggtcc tcgagagacg ttccttttca ccctttgaca tctggagcgg
     7381 gtcgcactct atcccaccct tacccccacc atagggtatg ccggcgaggg aacacttcca
     7441 ggtcatgagc atgcccaggg cttcgcattc atcaaggttc acatcggggt ggaacctggt
     7501 gccgcccttg gccggcccga tagccgtgga atgctggata cggtagccgt taaagacctt
     7561 ggtggttccg tcgtccatca cgacggggac cgaaacggac aatttccgct cggagttgcc
     7621 gagtatctcg accaggctgt cttcgagccc cagttcctct gccgcgccat agaagttttt
     7681 caaagcgata tcaaggagca cgttctctga agtcctctta cccattcaaa cagcacctcc
     7741 gcattcatct gatgacccgg gcacccc
//
LOCUS       DAOSRG010000041         7747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_8862_length_7747_cov_11.043032, whole genome shotgun sequence.
ACCESSION   DAOSRG010000041 DAOSRG010000000
VERSION     DAOSRG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8862_length_7747_cov_11.043032"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1327)
                     /locus_tag="PLM30_02730"
     CDS             complement(17..1327)
                     /locus_tag="PLM30_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR54118.1"
                     /translation="MSIEWIPPIMLFFVVLTMLLGVHVAWAMGGVAVVSIILAWNFNS
                     LFIVISSVYGVMTSSFYVALPLFIAMGVILERSGIADDLYQTFYVWAGRVKGGLAVAT
                     VLICVIFAAMTGLTGAACITMGLIALPSMLKRGYNKDLAIGTVIAPATLGILIPPSVV
                     MIMLGTIARVSVGKLFFAGLVPGLCLAALFIIYILIRGFLKPELCPSIEVEVSLKDKL
                     IALKAVILPFLTIFGVLGAIFFGIATPTESASIGVFTILLSTVFNRTFNWKLVFESSL
                     ETFRLTVLNIWIIFGALVFASTFTALGGIELMEKLIMAFHMTPWMVMFIILVILFILG
                     MFIDPFAIIFLVGPLTFPMMDKLGFDVIWYAIVFIMLICIGYITPPFGANLFYMKSVA
                     PPDVSMGDIYHSSWPFVIVMLIGVVLVIIFPDIVLWLPNKMIVH"
     gene            complement(1327..1845)
                     /locus_tag="PLM30_02735"
     CDS             complement(1327..1845)
                     /locus_tag="PLM30_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOR54119.1"
                     /translation="MKDTLNKIWKAIDMVNERTGVIISLLFFPGWFLVLYSVVVRYFF
                     NAPSNWAGDLAKFLFAIYFMMGGAYCLMNNSHIRVDVIYNLFGYKTRRLIELLVVIPL
                     LFAFAFPLVWQGSAFAWKAVKYMELSPPPTNLPVFPVKLAIPIAGGLFLLQALADLYR
                     FCFDKNAKEETR"
     gene            complement(2044..3711)
                     /locus_tag="PLM30_02740"
     CDS             complement(2044..3711)
                     /locus_tag="PLM30_02740"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOR54120.1"
                     /translation="MLSDTSIAQNAVLKPIDQVAADLGVPEQYVIPYGKTKAKIDLKF
                     LNELEEIPDGKLVLVTATTPTPAGEGKTTTTIGLAQALVKMGRKAVVCLREPSLGPCF
                     GVKGGAAGGGYSQVLPMDEINLHFTGDIHAVSSAHNLLSALVDNHLQQGNRLNIDPRR
                     VVFRRVMDMNDRSLRQIVIGLGGKGNGVPRENGFDISVSSEVMAILCLAGNLKDLKER
                     LAKIVVGYTYSSQAVTARDLKAEGAMTALLKDALNPNLVQTIEHVPAFVHGGPFANIA
                     HGTNSLSATKMALKLGDVAVVEAGFASDLGGEKFFDIACRYGEFSPRCVVLVTSVRAL
                     KMHGGLNKENQKEENIPALARGFENLDAHIENLSQFGVPVVVALNRFPTDTENEMGEV
                     IRHVRAKDAEVALSEVWEKGGDGGSELAEKVLKALEKPAGYRPLYDLDLSLTEKIELI
                     ADRVYGAAGVDFTDPVRAALEDLEVGGYGKLPVCMAKTQMSLSDDPKKIGRPKGWRLT
                     VREVRLSAGAGFVVPICGTIMTMPGLPRVPAAESVDVDDQGRVTGLF"
     gene            complement(3772..4317)
                     /locus_tag="PLM30_02745"
     CDS             complement(3772..4317)
                     /locus_tag="PLM30_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54121.1"
                     /translation="MGAVRSLIENEDIRNEDVEKITIWQPSYFAENFIAFPPSSSVDT
                     ASSLKYVVGAYMILRKIGVEWYEKYQEIMAAPAFKEISGKIEIIKDEGLQALFDKENV
                     VKGKVEIRTRKRVFEKEMRLEELKGNPRNNPMSFEDVKEKFLELSAPVIGEDKCRSFL
                     GSLDKDDPSLRIPELMRSLVL"
     gene            complement(4336..5169)
                     /locus_tag="PLM30_02750"
     CDS             complement(4336..5169)
                     /locus_tag="PLM30_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOR54122.1"
                     /translation="MGQITSEFMRQVQRTSFDGFSEKTIRETKKALIDTIGCMLAGTR
                     SPIGKATMEVFEGFGGQPESSVIGSKRKVPVAIAAYINAETCVGPDLSDNYQPESVIL
                     SHPGEAVIPAVLALGEKKGCTLRDLYTAVVVGYETAGRYALAIEPRRPEVYSFSTHYT
                     LAAAVGCGKILGFSDDEMEKNLGMAGALASLPVTGQMWGFRKRPASWHRDMPGHSNFS
                     AVIASQYIRAGFEGSRSLLDPETEFYKIAGSDHFNPDHLLKDWGKKYVIDELTFKQVP
                     T"
     gene            complement(5207..6592)
                     /locus_tag="PLM30_02755"
     CDS             complement(5207..6592)
                     /locus_tag="PLM30_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOR54123.1"
                     /translation="MSLTKQVVENLQGTRFDDFPEKTVEYARFVILDSVGSALAGSGT
                     EVGRIFYGLHNDDEAGPSLVWGSDRFSSLSAACFINSSLSQILDFDDTYEINTLAVSH
                     PGPAIVPLALTMADRYDIKGGELIRAIVLGYEMCMRFSSAIEPRYDDYFGFSNSQMIG
                     AVTSSSILLGLSDEEFINSYGLAVSTSTVPTTKSMWSLEARPMSWVKDGVGFVSEAAL
                     MCSRMASKGFRATREGLDPGDKFSVLCGSRDYKHSKLTEGFGDPYLIENISFKPYPTC
                     RFMQSILDIVDKEIVVKGIAGNDVEKIQVFITPYLKNSFDVRDPLTMIDAQFSLPYAV
                     AMLISGRLPSLAWYAPEAMKDPYTRTLVNKVELIPDEAIEKGRKESSELKNRVRIILR
                     NNEEYFFETGFAKGHPRNPFSKEDHISKFRQNLSGLFGARKIETLAEMILGMGKTTDI
                     RNMVSRFRPDD"
     gene            complement(6721..7383)
                     /locus_tag="PLM30_02760"
     CDS             complement(6721..7383)
                     /locus_tag="PLM30_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54124.1"
                     /translation="MQFDLGQENLSTRVAEYIKDYLLFSGRFGNGQKVTEHEVARALG
                     ISRSPIREAFKELHNEGLLEFKPRKGSFVVKLSDQDVNELYLLRFWIESRLYEVIIQR
                     KTLTQEKYDEIFQLIKKIESIVGSDLDHHKKTIEYSRCALEIHTGLWSLSNMKWAVQV
                     LTRLHNQMRLAMIQDLDYAPDLRKNALLHYEILDALKNEQYEEAVRALREDMKLYMNG
                     IL"
     gene            complement(7673..7747)
                     /locus_tag="PLM30_02765"
     tRNA            complement(7673..7747)
                     /locus_tag="PLM30_02765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7713..7715),aa:Ala,seq:ggc)
BASE COUNT         1836 a         2158 c         1942 g         1811 t
ORIGIN      
        1 cctcatgcta ttttgatcaa tggacgatca tcttgttggg aagccataaa acgatgtccg
       61 ggaatattat gaccagtacg acgccgatca gcatgactat gacgaaaggc caggaggagt
      121 ggtagatatc tcccatggat acatcgggcg gcgccaccga cttcatgtag aacaaattgg
      181 caccgaaggg gggcgtgata tagccgatgc agatcagcat tatgaacacg atggcgtacc
      241 agatcacatc aaacccgagt ttgtccatca tcgggaacgt caaggggccg acgaggaaaa
      301 tgatggcaaa ggggtcgatg aacatcccca ggatgaatag tatcaccaaa attatgaaca
      361 ttaccatcca gggagtcatg tgaaacgcca tgatcagctt ttccatgagt tctatcccgc
      421 cgagggcggt aaaggtggaa gcgaagacca gggccccgaa aatgatccag atgttcaaaa
      481 ccgtcagcct gaaagtttcc agggacgatt caaaaacaag tttccagttg aaagtccggt
      541 tgaaaaccgt tgataacagg atagtgaaga ccccgatgga ggcggattcg gtgggggtcg
      601 ctataccgaa gaaaatggcc cctagaacgc cgaaaatcgt aaggaacggt agtattaccg
      661 ctttaagggc gatcagctta tccttgagag agacctccac ctcgatggaa gggcaaagct
      721 cgggtttgag gaaacctctt attagtatgt aaatgatgaa cagggccgcc aggcaaagcc
      781 ccgggaccag gcccgcgaag aaaagtttcc ccacggagac acgggcgatc gtcccgagca
      841 tgatcatgac gacgctcggc ggaataagaa ttcccagggt cgcaggggcg atcactgtcc
      901 ctatcgcgag atccttgttg taacccctct tgagcatgga cggcaacgct atcaggccca
      961 tcgtgatgca ggcggctccg gtcaagcctg tcatggccgc gaatatcacg cagatcaata
     1021 ccgtggccac ggcaagaccg cctttgactc gccccgccca gacgtagaag gtctggtaaa
     1081 ggtcatcggc gatccccgaa cgctccagta tgactcccat agcgatgaat aagggtagcg
     1141 caacgtaaaa ggaactcgtc atgaccccgt agaccgaact gatgacaatg aagaggctgt
     1201 tgaagttcca cgccaggatg atggatacta ccgccacccc tcccatggcc cacgctacat
     1261 gcacccccaa taacatggtc aggacgacga aaaatagcat tatggggggg atccactcaa
     1321 tgctcattac cgggtttcct ccttggcatt cttgtcaaag cagaagcgat acaggtccgc
     1381 cagcgcctgg agcaggaaaa ggccccccgc tatagggatg gcgagcttga cggggaaaac
     1441 aggcaaattc gtcggagggg gggatagttc catgtacttc accgctttcc aggcaaaagc
     1501 agaaccctgc cagacgaggg ggaaggcaaa ggcgaaaagt aaggggatca ccaccagcag
     1561 ttctatcagc ctcctcgttt tataaccgaa aaggttgtat atcacatcga ccctgatatg
     1621 ggaattgttc atcaggcaat atgccccgcc catcatgaag tagatcgcga acaggaattt
     1681 tgccaggtca cctgcccagt tactgggagc gttgaaaaag tacctcacca ctaccgagta
     1741 gaggaccaaa aaccatcccg ggaaaaacaa aaggctgatg atcacccctg tcctttcatt
     1801 taccatatct atcgctttcc agattttgtt caaggtatcc ttcaaatcag aaaacctccc
     1861 cttccatagg aaatccttca acaaaagcgt ttcagctgac ggccagacat tttgtggatc
     1921 taagggctga caagtcaata aagcaagaca aaattaaatt attaacctgt ctcgatcacc
     1981 ggggcgaaga gatcgcaccc gggttggccc cacggatctc gctccctggt cttccctgtg
     2041 ggctcagaaa agtccggtga cccgcccctg gtcgtcgaca tcgacgctct ccgccgccgg
     2101 cacacggggt aatcctggca tagtcattat tgttccgcag atgggcacca caaagcccgc
     2161 gcccgctgaa agccgtactt cccttacggt taaccgccaa cccttggggc gcccgatctt
     2221 tttagggtcg tcggaaaggc tcatctgtgt tttcgccatg cagacaggca gtttcccgta
     2281 ccctcccacc tcgaggtcct ccagggccgc acggaccggg tcggtgaagt ccacgccggc
     2341 ggcgccgtag acacggtccg cgatgagttc gatcttttcc gtcaaggaga ggtccaggtc
     2401 gtacaacggc cggtaaccag ccggtttttc caaggccttc aacaccttct ccgcaagttc
     2461 cgatccccca tccccgccct tttcccacac ttccgacagg gcgacttctg catccttggc
     2521 gcggacatgc ctgatcactt cccccatctc attttccgta tccgtgggga aacgattcag
     2581 agccaccact acgggtacac cgaactggga gaggttttca atatgggcgt ccaggttctc
     2641 aaagcccctg gccagagccg ggatgttttc ctccttctgg ttctccttgt tgagcccccc
     2701 gtgcattttg agggccctga cggaggtgac cagcactacg catctcggcg agaactcccc
     2761 gtaacggcag gcgatatcga agaacttctc ccccccgagg tccgaagcga agcccgcctc
     2821 gacgacggcc acgtctccca gtttcagcgc catcttcgtg gccgaaaggg agttggtccc
     2881 atgggcgata ttcgcgaagg gaccgccatg aacgaaagcg gggacatgct ctatcgtctg
     2941 gaccaagtta gggttcaggg cgtctttcaa tagagcggtc atggccccct cggccttgag
     3001 gtcacgggcg gtgaccgcct ggctggaata ggtgtagccc acgactattt tggccaggcg
     3061 ctccttgagg tccttgaggt taccggcgag gcatagaatg gccatgacct ccgatgagac
     3121 gcttatgtcg aagccgttct ccctgggtac tccgttcccc ttgccgccaa gtccgatcac
     3181 tatctggcgg agcgaccggt cattcatatc catgaccctg cggaaaacga ccctcctggg
     3241 gtcgatgttc aacctgtttc cttgctggag atgattatcc accagggccg acaggaggtt
     3301 gtgggccgac gagacggcat ggatatcgcc ggtgaagtga aggtttatct cgtccatggg
     3361 aagcacctgc gaatacccac ctccggccgc accgcctttc actccgaagc aggggcccag
     3421 ggaaggctcc ctgaggcata cgactgcctt tctgcccatc ttcaccaagg cctgggcgag
     3481 ccctatggtc gtcgtcgtct tcccctcccc cgcaggggtg ggtgtcgtcg cggtgacgag
     3541 gaccagtttc ccgtcgggta tctcctccag ttcgttaaga aatttcaggt cgatcttcgc
     3601 ttttgtctta ccatagggaa ttacatattg ctcaggtacc ccaagatccg ccgccacctg
     3661 gtcgatcggt ttcaagactg cattttgagc gatagaagtg tccgatagca tcaattatca
     3721 ccttgatgtg acaagcctcg cctttaggcg gtctttcaaa aaaccaattt tctacaatac
     3781 aagcgacctc atgagttccg gtatcctgag cgaaggatca tccttatcca gcgaccccag
     3841 gaatgaccgg catttgtcct ctcctatcac cggagcggag agttcaagga atttttcctt
     3901 cacgtcttca aagctcatag ggttattccg ggggttgcct ttgagttctt ccagcctcat
     3961 ctccttttcg aaaacccttt ttctggtccg gatctcgacc ttccccttga caacattctc
     4021 cttgtcaaaa agggcctgca acccttcgtc ctttattatc tcgatcttcc ccgagatctc
     4081 cttgaatgcc ggggccgcca tgatctcctg gtatttttca taccattcga cccctatttt
     4141 cctgaggatc atgtaagcgc ccacaacgta cttcaggctg gaagcggtat ccacagaaga
     4201 agaaggcggg aaggcaatga agttttcggc gaaataagag ggctgccaga tcgtgatctt
     4261 ctccacgtcc tcgttcctga tatcctcatt ctcgatgagg gagcgcacgg ctcccactcc
     4321 agtctggagg aagtatcagg tggggacctg cttgaaggtc agttcgtcga tcacgtattt
     4381 tttcccccaa tccttgagaa ggtggtcggg gttgaaatga tcggaaccgg ctatcttgta
     4441 gaactccgtc tcagggtcca gcagggagcg ggaaccttca aagccggccc tgatgtattg
     4501 ggacgcgatc acggcggaga agtttgagtg gccgggcata tcccgatgcc aggaggccgg
     4561 ccttttccta aatccccaca tctgtcccgt gacaggcaaa gaggccaacg cccccgccat
     4621 accgaggttt ttctccatct catcatcgga aaaacccaag atcttgccgc aacccacggc
     4681 cgccgccagg gtgtaatggg tcgaaaagct gtagacttcg ggcctccgtg gttcgatggc
     4741 caaggcgtat ctcccagcgg tttcataacc aaccaccacg gcggtgtaaa ggtccctgag
     4801 ggtacagcct tttttctcac ccagggccag cacagcgggt atcacggctt ccccagggtg
     4861 ggaaaggata accgattccg gctggtagtt gtcgctcagg tcgggtccga cacaggtctc
     4921 ggcgttgatg taagcggcta tggctacggg tacttttctc tttgaaccga taacggaaga
     4981 ctcgggctgt ccaccgaaac cctcgaagac ctccatcgta gccttgccga tcggtgatct
     5041 cgtccccgcc aacatgcaac ctatcgtgtc tatcagggct tttttcgttt cacgtatagt
     5101 tttctccgaa aaaccatcga aggatgtcct ttgcacctgg cgcatgaatt cgctcgtgat
     5161 ctgacccatg aagctcttcc tcctcaataa agatttttat cggcgctcaa tcgtcgggac
     5221 ggaaccggga caccatattc cttatgtcag ttgttttccc cattccaagg atcatctcgg
     5281 ccagggtttc aattttcctc gccccaaaaa gtccgctcag attttgtcgg aacttcgata
     5341 tatggtcttc cttggaaaag ggattgcgcg ggtggccctt ggcaaaaccc gtttcgaaaa
     5401 agtactcctc gttgtttctt aagatgatcc tcaccctgtt tttcagttcg ctgctttctt
     5461 ttctcccctt ttctattgct tcgtctggta taagttcgac cttgttcaca agtgtccttg
     5521 tataagggtc tttcatcgct tccggtgcgt accacgccag cgaagggagc ctgccggaga
     5581 tgagcatggc taccgcgtag ggcaaactga actgcgcatc gatcattgtc aggggatccc
     5641 tgacatcaaa ggaattcttc aaatagggag tgatgaaaac ctgtatcttt tcaacatcat
     5701 taccggcaat gcccttgacg acaatttcct tgtcgacgat gtcgaggatc gattgcatga
     5761 acctgcacgt cgggtaaggt ttgaaactta tattctcgat caggtaagga tcgccgaagc
     5821 cctccgtcag tttcgaatgc ttgtagtccc gggaaccgca gagcacggaa aacttatcac
     5881 ctgggtccag cccctctctc gtggcgcgga agcccttcga tgccatcctg gaacacatca
     5941 gcgccgcctc ggaaacaaag cccaccccat ccttgaccca cgacatgggt cttgcctcca
     6001 ggctccacat tgacttagtg gtcggcaccg tcgaagtgga aacggcaagc ccataggaat
     6061 tgataaactc ttcatcgctc agaccgagaa gtatagagga ggacgttaca gccccgatca
     6121 tctgggaatt ggaaaaaccg aaataatcat catacctggg ttcgatggcg gaagaaaacc
     6181 tcatgcacat ctcgtatccc agcacaattg cccggatcaa ttccccaccc tttatgtcgt
     6241 atctatccgc cattgtcaga gcgagaggga ctatggcagg gcccgggtgg gaaacggcga
     6301 gggtgtttat ttcataggta tcatcgaaat cgaggatctg ggacaaggag gaattgatga
     6361 agcaggctgc actcagggaa gaaaaacgat cagagcccca gaccaacgat gggcccgctt
     6421 catcatcatt gtggaggccg tagaagatcc taccgacttc cgtcccggaa ccggccaagg
     6481 cggaaccgac agaatcaagg atcacgaacc gggcatattc caccgtcttt tcaggaaagt
     6541 catcgaacct ggtgccttga aggttctcta ctacttgttt ggttaaactc atgaacccaa
     6601 gcccctctct tccccttttc ttctcccgtc ccccgccatg agtttctgtg aagtggatcc
     6661 tttttcaatc cctttcatca tttgaggaac agccctcttg aagtcgtgag acgggggact
     6721 tcagaggatc ccgttcatat atagtttcat gtcctcccgg agcgctctta cggcttcttc
     6781 gtattgctcg ttcttcaggg catcgaggat ctcataatgc aacagtgcat tttttcgaag
     6841 gtccggcgca taatccaggt cctggatcat cgcgagtctc atttgattat gcagcctcgt
     6901 taggacctga acggcccatt tcatattcga caaggaccaa aggccagtat ggatctccag
     6961 tgcgcatcgg gaatattcta ttgttttttt atggtggtcc aggtctgatc caacaatgct
     7021 ttctattttt ttgatcaatt ggaaaatctc gtcgtatttt tcttgggtta aagtcttccg
     7081 ctggatgata acctcgtaca aacgcgactc tatccagaac cggagcaaat aaagttcatt
     7141 cacgtcctga tcagagagct tgaccacgaa agaacctttc ctgggtttaa actcaagaag
     7201 gccttcattg tgcaattcct tgaaagcttc ccttatcggg gacctgctga tccccaaggc
     7261 cctggccacc tcgtgttccg tcactttctg cccatttcca aatctacccg agaataataa
     7321 ataatcttta atatattctg cgaccctcgt gctcaggttt tcttgcccca gatcgaattg
     7381 catcgcatca tcctcctttg gtcgacaata gactgttcta gacaaattat cttaaaaaaa
     7441 aacctttgtc aagaaaagac accggaaacg ggcgattgta ttgtacccgt aaagcaaagg
     7501 gagaaggcct gccttatgcc tgggcgggct gtccccgaag gggtaacaaa ggtgtcagtc
     7561 cctttgagga gaaaaagtga gagctctacc cctgaaagga ccgggaggct gtgggaccct
     7621 taataagaaa aaagcggtcc ctggatcttc ccagggaccg cttttacaat cctggtggag
     7681 gttgggggat tcgaaccccc gaccccttgc atgccatgca agtgctctcc cgctgagcta
     7741 aaccccc
//
LOCUS       DAOSRG010000042         7296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_9521_length_7296_cov_10.204806, whole genome shotgun sequence.
ACCESSION   DAOSRG010000042 DAOSRG010000000
VERSION     DAOSRG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9521_length_7296_cov_10.204806"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PLM30_02770"
     CDS             <1..1666
                     /locus_tag="PLM30_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54125.1"
                     /translation="LSSACAVVAPWLLRNIVDDVLISKDMAMLNLVAVGLVLLYLAKS
                     LFFYGQQYLMNWVGQRVVVDLRLRLYDHMQRMSFRYLYGRRVGEMLSRITNDVTILQN
                     MITTVFIDIVVQGLSFAGMIAFLLYINWKLSLLSFLVLPLAAFVLDVASRRLRVVGHD
                     VQKQLAGLSAIAAEALSAIRIVRLFATEEQEYGRFESQSNAHFRALMRGVQTNAALTG
                     IVEVILITALAAILWFGGRLVVSGELTPGELIAFLGYLALLVQPVRVFSRVVAQMQQG
                     LAAADRVFEILDVESEVKPPKHPEKVDPIRGEIAFQDVSFAYNEGAWVLEDVSFRVSP
                     GEKVAIVGPTGAGKSTIADLITRFYDPQQGAILIDGKDIRRLDLKGLRRKIGVVPQDP
                     VLLKGSIAFNIAYGCEDLSEGDIESAAKVAGIHDFIMSLPDGFKAEVGERGVTLSGGQ
                     RQRIAIARAIVRNPRILIMDEATSSLDAAVEQEIQEAMKKAMSGRTSIVIAHRLATVR
                     EADRILVIDRGRIVEEGSHEILKDAGGLYSRLCCLQFGENHGSPAE"
     gene            1596..3935
                     /gene="lpxK"
                     /locus_tag="PLM30_02775"
     CDS             1596..3935
                     /gene="lpxK"
                     /locus_tag="PLM30_02775"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOR54126.1"
                     /translation="MPGDSTPGSAASSSGRTMGALLSSYFRHIRGERESFPWRLLAPL
                     GWLVEAGSRARNFSYDHGLSASREMPIPVVSVGNITHGGTNKTPFVEMLARVFVDAGL
                     RPGIVMRGYGRKQKGCLLAGECEPSRDLLGDEALLLSGRLSGVPIAVSADRAGGVLRL
                     AGEGVDVAIADDGFQHRKMGRDVNVVLIDATCPFGNGRLMPAGMLRENPGSLERAHLV
                     VITKADQVAEGRVEEISAAIRGITGEEPFLSSLALAGWSRFLGPREGLEECPPPMLPA
                     AAFSAIGNPHSFGSFLEQLGFPIVFHEIFRDHHIFTESELAAIGGKMTSLGARSLICT
                     EKDIFNLPPDWSPAVPVYVPRVHAVLADGDSFFAALALHLRPRVVVASNGHGEDAIGS
                     LLAERLQSRFPLFEVSGFPLVGRGQEYRSRSVRVLSPPSETPSGGVVKYSLRDLIRDI
                     RSGLFRHIEAQAEAWSGLRGRLRTVLCVGDVYLFLHSLWGQGTMPVLLATAKSERLHG
                     HWGLEKWLLKRRCRRVFTRDPETARELSERKADAVFKGNPIMDLAGEAVGKPGGHRPF
                     GVLLLPGSRERAYDDLGLLLGAAEVIALEEDCRFEMVVASTLDRERLLEKAPSWRVDS
                     PDVIRHEKGKPEVRLFSGEVSRAASRSDILIGLGGTANQICAGMGLPVVSIDEKGKRV
                     QKKLLGEAESLVTADPVSLAREALAILGDPARYERMSRAGMERMGGGGALEAVIEYAS
                     RTLGWEKRQEVCRVFGAYVEKNSGRTGSGPEEHGDDRSW"
     gene            3929..4762
                     /gene="kdsA"
                     /locus_tag="PLM30_02780"
     CDS             3929..4762
                     /gene="kdsA"
                     /locus_tag="PLM30_02780"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOR54127.1"
                     /translation="MVKSFSAGKVFFGDGGLVLVAGPCVLEDRETALSIAREVSGICG
                     RLGIPYVFKASFDKANRTSIHSFRGPGLEKGLQWLAEVKESVGCPVLTDIHLPGQAER
                     AAEVVDIIQIPAFLCRQTDLVVAAAATGRAVNIKKGQFLAPVDMGQAVSKCLEAGNDK
                     VILCERGSTFGYGQLVVDMRSLAIMRSLGYPVMFDATHSVQMPGAQGTSSGGDRRFVP
                     VLARAAAAAGIDALFLETHPDPDSAKSDGPNMVSLDSLENLLRKVLAIHRAAGVAEGE
                     R"
     gene            4766..5773
                     /locus_tag="PLM30_02785"
     CDS             4766..5773
                     /locus_tag="PLM30_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOR54128.1"
                     /translation="MVSLPRERDNVVFDDERLLEVGRRVLRMEAEEIARAAARVGREL
                     VEAARIISRCEGRLVVVGMGKSGLVGRKIAATLASLGTPAFFLHAAEGSHGDLGMVCR
                     EDAGLFLSNSGQTREILEVLPFFRRLGAPVIAMTGNPSSRLAGDSDVVIHSGVEKEAD
                     PLGLAPTTSTTLQLALGDALAGMVTELRGLRPEDFALFHPGGSLGRRLLLRVSDVMST
                     GERVPRVTSGASVREALFEITSKGFGATIVEDERGMATGIFTDGDLRRLLEDRGVDSL
                     SLPIEEVMTRDPRTISPDRLAAEAMHIMEEREVSCLVVSEEGRATGIIHIHELLKAGV
                     V"
     gene            5685..7049
                     /locus_tag="PLM30_02790"
     CDS             5685..7049
                     /locus_tag="PLM30_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOR54129.1"
                     /translation="MRYPAWWSPKRGGPRASSTSTNSSRREWSDLASLLYRSFISTAY
                     CAAFPWLAKKYATGLGERRGLYSEEKRDRLPDSPLWFHAVSVGEVQSAWPLLKILARE
                     GTGPGEILLSTTTPTGREMAYRVAGGLFHEHLYYPWDVPWIVRRSLDAIRPRAFAVLE
                     TEIWPLMLDELRERGIPAILVNGRFSDRTAGNIRSHLHFWRRVYKSFSLLLVRSERDR
                     ELLLEIGLEERRVQVTGDCKIDAILLREEEADLGWARAVVGETGPVFLAGSTHPGEEE
                     IVLEAFQRLKAKIPSARLIVAPRHPQRSTRVLELAKRAAPAALLSEVEREKKGKEAPW
                     DILVADRIGVLFDLYGVVDGAFVGGSLVDRGGQNIMEPAAFGIPLSHGPFMRDFREAS
                     ESLGEKGVSVVVRGAGDMAAHWESCLKPEIKGAAREGARRFFSASSGAAKASVEAIRK
                     EIGP"
     gene            7046..>7296
                     /locus_tag="PLM30_02795"
     CDS             7046..>7296
                     /locus_tag="PLM30_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54130.1"
                     /translation="MKVNEKLLDQFYGNLDDRSRASLDAAVERIVSAKAAGGKIVVAT
                     GSGPNIHEGVTTLIAELMDKGMVDGVTTSSAVIGHEMGGA"
BASE COUNT         1315 a         2211 c         2452 g         1318 t
ORIGIN      
        1 gctctcatcg gcctgcgcgg tcgtcgcgcc ctggctcctc aggaacatcg tggacgatgt
       61 cctgataagc aaggacatgg ccatgctgaa cctcgtcgcg gtggggctgg tgctccttta
      121 cctcgccaaa agtctcttct tttacggcca gcaatacctg atgaactggg tggggcaacg
      181 ggtcgtggtc gacctgaggc ttcggcttta cgaccacatg cagaggatgt ccttccgcta
      241 cctttacggc aggcgtgtag gggagatgct ctccaggata acgaacgatg tcaccatcct
      301 gcagaacatg ataaccacgg tattcataga catagtcgtc caggggctga gttttgccgg
      361 gatgatcgcc ttcctgctgt atataaactg gaaactctcc ctcctgtcct tcctggtcct
      421 cccgctggcc gccttcgttc tggatgtggc ctccaggcgc ctcagggtcg tcggccacga
      481 cgtccagaag cagttggcgg ggctttcagc catagcagcc gaggcccttt cggctatccg
      541 tatcgtgcgt ctcttcgcca ccgaggagca ggagtacggc cgttttgaat cgcagagcaa
      601 cgcccatttc agggccctca tgaggggagt tcagaccaac gccgccctca ccgggatagt
      661 ggaggtcatc ctcataaccg cgctggcggc gatactctgg ttcgggggaa ggctggtcgt
      721 ctcgggtgaa ctcacacccg gcgaactgat agccttcctg ggttacctgg cgctgctcgt
      781 ccagcccgtc cgggtctttt ccagggtggt cgcccagatg cagcagggcc tggccgccgc
      841 ggacagggtc ttcgagatcc tcgacgtgga aagcgaggta aagccgccga agcatcccga
      901 aaaggtcgac cctatcaggg gggagatcgc ctttcaggac gtgtccttcg cctacaacga
      961 gggagcctgg gtcctggagg atgtctcctt ccgagtatca cccggcgaga aggtcgccat
     1021 cgtggggccc accggcgcgg ggaaatcaac catcgccgac ctcatcaccc gtttctacga
     1081 tccccagcag ggcgcgatcc tcatcgacgg aaaggacatc aggaggcttg acctcaaggg
     1141 gcttaggagg aagatcggcg tcgtccccca ggaccccgtg cttttgaagg gcagcatcgc
     1201 cttcaacatc gcctatggct gcgaggacct ttccgagggg gacatcgaaa gcgccgcgaa
     1261 ggtcgccggg atacacgatt ttatcatgtc tctgccggac ggtttcaaag ccgaggtggg
     1321 tgaacggggc gtcaccctca gcggcggtca gcgccagagg atcgccatcg ccagggccat
     1381 cgtccggaac cccaggatac tcatcatgga cgaggccacc tcctccctgg acgccgccgt
     1441 ggaacaggag atccaggagg cgatgaagaa ggccatgtcg ggcaggacct cgatagtcat
     1501 cgcccacagg ttggccactg tcagggaagc cgaccgcata ctggtgatag accgtggaag
     1561 gatcgtcgag gagggaagcc acgaaatcct gaaggatgcc gggggactct actccaggct
     1621 ctgctgcctc cagttcgggg agaaccatgg gagccctgct gagtagctac ttcaggcata
     1681 tccgtggcga aagagaaagc ttcccgtgga ggcttctggc cccactgggc tggctggtcg
     1741 aggccggctc ccgcgccagg aatttctcct acgatcacgg cctgtcggcc tcccgggaga
     1801 tgcccatccc cgtggtgagc gtcggcaaca tcacccacgg cggcaccaac aaaactccct
     1861 ttgtcgagat gctcgccagg gtcttcgtcg atgccggcct caggccgggg atagtcatgc
     1921 ggggctatgg gcgcaagcag aagggatgcc tcctggcggg ggagtgcgag ccgagcaggg
     1981 atctcctggg cgacgaggcc cttctacttt ccggaagact tagcggcgtg cccatcgcgg
     2041 tttcggccga ccgagccggg ggagtcctgc ggctggccgg agagggcgtc gacgtagcga
     2101 tagccgacga cggtttccag cacaggaaga tggggcggga cgtaaacgtg gtgcttatcg
     2161 atgcgacctg tcccttcggg aatgggcggc ttatgcctgc cgggatgctc cgggaaaacc
     2221 cggggtcgct ggagagggct cacttggtgg tgatcaccaa ggccgaccag gtggccgaag
     2281 ggcgcgtgga ggagatatcg gccgccatcc ggggcatcac cggggaggaa cctttccttt
     2341 cctcgctggc cctcgcggga tggtcccgtt ttctcggccc ccgggagggc cttgaggaat
     2401 gcccaccgcc catgcttccc gcggcggcct tttcggccat cggcaacccc catagtttcg
     2461 gttcttttct cgaacagctc ggcttcccca ttgtcttcca cgagatcttc cgcgaccatc
     2521 acatcttcac ggaatcggaa ctggccgcca tcggcgggaa aatgacctcc ctgggggccc
     2581 ggtctctgat ctgcaccgag aaggatatct tcaacctccc cccggactgg tcgcccgccg
     2641 tcccggtcta cgttcccagg gtccacgcgg tcctggcaga cggggactcc tttttcgccg
     2701 cccttgcact acatctcagg cccagggtcg tcgtcgcctc caacgggcac ggagaggacg
     2761 ccataggcag cctgctcgcc gaacggcttc aaagcaggtt tcccctcttc gaggtttcgg
     2821 gcttccccct cgttggcagg gggcaggagt accggtccag gtcagtcagg gtgctctcac
     2881 cccccagcga gacgccgagc gggggcgtgg tcaagtatag tttgagggat ctcatccggg
     2941 acatccggtc gggtctcttc aggcatatcg aggcccaggc cgaggcctgg agcggtctca
     3001 gggggcggtt gaggaccgtc ctctgcgtcg gcgatgtcta ccttttcctg cacagtctct
     3061 gggggcaggg caccatgccg gtgctcctgg ccacggccaa gtccgagcgc cttcacggcc
     3121 attgggggtt ggagaagtgg ctcctcaaaa ggcgctgcag gagggttttc accagggatc
     3181 ccgaaaccgc ccgggaactg tctgaaagaa aggccgacgc ggtcttcaag ggaaacccca
     3241 taatggacct cgccggagag gccgtcggca aacccggagg gcatcggccc ttcggggtcc
     3301 tgctcctccc gggaagccgg gagagggcct acgacgacct ggggctcctg ctcggtgccg
     3361 ccgaggtcat agccctcgag gaggactgcc ggttcgagat ggtcgttgct tccaccctgg
     3421 atagggagag attgctggag aaggctccct cctggagggt cgacagtccc gatgtcatca
     3481 ggcacgaaaa gggcaaaccg gaggtgcgcc tcttttccgg cgaggtctcc cgggcggcgt
     3541 cccgctccga cattcttatc ggtcttgggg gcacggccaa ccagatctgt gccgggatgg
     3601 gcctgccggt ggtctccatc gacgaaaagg gcaagagggt gcagaaaaaa ctcctgggcg
     3661 aggcggaatc gctggtcacc gccgacccgg tttccctggc ccgggaagcc ctggccatcc
     3721 tcggggatcc ggcccgttac gagaggatgt cccgcgcggg gatggagagg atgggcggtg
     3781 ggggtgccct ggaggccgtg atagaatatg cttcccggac gctgggatgg gagaaaaggc
     3841 aggaagtgtg ccgggttttt ggcgcctatg ttgaaaagaa ttcgggaaga acgggttccg
     3901 gacccgaaga gcatggagat gatcggtcat ggtgaaaagc ttttccgccg gcaaggtttt
     3961 tttcggtgac ggggggctgg tcctcgtggc gggaccctgc gtcctcgagg accgggagac
     4021 ggccctttcc atagcgcggg aggtttcggg tatatgcggc aggctgggca taccctacgt
     4081 cttcaaggcc tccttcgaca aggccaacag gacctccatc cacagcttca ggggtcctgg
     4141 cctggagaag ggccttcagt ggctcgccga ggtgaaggag tcggtcggct gtcccgtcct
     4201 gacggatata cacctccccg gccaggccga aagggcggcc gaggtggtgg atattatcca
     4261 gataccggcc ttcctctgca ggcagaccga cctcgttgtc gccgccgcgg ccacgggcag
     4321 ggcggtgaac atcaagaagg ggcagttcct cgccccggtg gacatggggc aggcggtctc
     4381 caagtgcctc gaggcgggca acgacaaggt catcctctgc gagaggggga gcaccttcgg
     4441 ctacggccag ctggtggtcg atatgcgctc cctcgcgatc atgaggtccc tggggtaccc
     4501 ggtgatgttc gacgccaccc acagcgttca gatgcccgga gcccagggga cgtccagcgg
     4561 gggggacagg aggttcgtcc ccgtccttgc cagggccgcc gcggcggcgg gtatcgacgc
     4621 ccttttcctt gaaacccacc ccgacccgga ctcggccaaa agcgacgggc cgaacatggt
     4681 ctccctcgac tcgctcgaga acctgctgcg gaaggtcctg gccattcacc gagctgccgg
     4741 cgtcgcggaa ggggagaggt gaccgatggt cagcctgcca agggaacggg acaacgtggt
     4801 ttttgacgac gaaaggctcc tcgaggtcgg ccggcgcgtc ctccgcatgg aggccgagga
     4861 aatagcccgg gccgccgccc gggtgggcag ggaactggtg gaggcggcca ggatcatcag
     4921 ccgatgcgag gggaggctgg tggtggtcgg gatgggcaag tcggggctcg tcggccgcaa
     4981 aatagccgcc acgctggcct cgctgggcac gccggctttc ttcctccacg ccgcggaggg
     5041 ctcccacggt gacctgggaa tggtgtgccg cgaggacgcg ggccttttcc tgagcaacag
     5101 cggccagacg agggagatcc tcgaggtgct tcccttcttc aggcggctcg gggcgccggt
     5161 gatagcgatg acgggcaacc cttcctccag gctcgccggg gactccgacg tggtgatcca
     5221 ctccggcgta gagaaggagg cggacccgct gggactcgct cccacgacga gcaccacgct
     5281 gcagctggct ctcggcgacg ccctggcggg catggtcacc gaactccggg gacttcgccc
     5341 cgaggatttt gccctctttc accccggcgg gtctctcggc aggcggcttc ttctccgggt
     5401 ttcggacgtg atgtcgacgg gagagagggt ccccagggtg acgtcgggcg cctcggtccg
     5461 ggaagccctt ttcgagatca ccagcaaggg cttcggggcc accatagtcg aggatgagcg
     5521 tgggatggcg acgggcatct tcaccgacgg ggatctcagg cggctcctcg aggacagggg
     5581 ggtggattcg ctctccctgc ctattgagga agtcatgacg agggaccccc ggaccatatc
     5641 ccccgaccgt ctggccgccg aagcgatgca catcatggag gagcgtgagg tatcctgcct
     5701 ggtggtctcc gaagagggga gggccacggg catcatccac atccacgaac tcctcaaggc
     5761 gggagtggtc tgacctggcg tccctccttt atcgctcctt catctcgaca gcctattgcg
     5821 ccgctttccc ctggctcgcg aaaaagtatg ccacggggct tggggagcgg aggggccttt
     5881 acagcgagga aaagagagac cgcctgccgg acagcccgct gtggtttcat gccgtctcgg
     5941 tgggggaggt gcagtccgcc tggccccttt tgaagatcct ggcccgggaa ggcacgggac
     6001 ccggggaaat cctcctttcg accaccaccc ccacggggcg ggagatggcc taccgggtag
     6061 cgggggggct tttccacgaa cacctttact acccttggga tgtcccctgg atcgtccggc
     6121 gctccctcga tgcgatccgc cccagggctt tcgcggtcct cgagacggag atctggccct
     6181 tgatgctgga cgaactacgg gaaaggggca tccccgccat tttggtcaac ggtcgattct
     6241 ccgacaggac cgccggcaat atccggagcc acctccattt ctggcgccgg gtgtacaaat
     6301 ccttctctct tctcttggtg cggtccgaaa gggataggga actgctcctg gagatcgggc
     6361 ttgaagagcg cagggtccag gtgacgggag actgcaaaat agacgccatc ctccttcgcg
     6421 aagaagaggc ggacctgggg tgggcgaggg cggtcgttgg tgaaacgggc cccgttttcc
     6481 tcgccgggag cacccacccg ggcgaggagg agatcgtcct ggaggcattc cagaggctga
     6541 aggccaagat tccttcggcc cggctcatcg tcgcgccccg ccacccccaa aggagtaccc
     6601 gggtcctgga actggccaag agagccgcac cggcggccct cctctcagaa gtggaaaggg
     6661 agaaaaaagg aaaggaagct ccctgggaca tcctcgtggc tgaccgcatc ggggtcctgt
     6721 tcgaccttta cggcgtcgtg gacggggctt tcgtaggggg aagccttgtg gacagggggg
     6781 ggcagaatat aatggaaccg gccgctttcg gcatacccct ttcccacgga cccttcatgc
     6841 gggattttcg ggaggcctct gaaagcctcg gtgaaaaggg ggtttccgtc gtggtgcggg
     6901 gcgccgggga tatggcggct cactgggaga gctgcctcaa accggaaata aagggcgccg
     6961 cccgtgaagg agcgaggcgg tttttctcgg cttcctcggg ggcggcgaaa gcgtcggtgg
     7021 aagcgatccg aaaggagatc gggccgtgaa ggtgaacgaa aagttgctgg accaatttta
     7081 cggaaacctg gacgatcgct cccgggcgag cctcgacgcc gccgtggaaa ggatagtgtc
     7141 cgccaaggcg gccggtggca aaatcgtggt ggccaccggg agcggtccca acatccacga
     7201 gggagtgacc accctcatag ccgaactcat ggacaaggga atggtggacg gcgtcaccac
     7261 cagctccgcc gtgatcgggc acgagatggg cggcgc
//
LOCUS       DAOSRG010000043         7242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_9602_length_7242_cov_11.483373, whole genome shotgun sequence.
ACCESSION   DAOSRG010000043 DAOSRG010000000
VERSION     DAOSRG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9602_length_7242_cov_11.483373"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..594
                     /locus_tag="PLM30_02800"
     CDS             298..594
                     /locus_tag="PLM30_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOR54131.1"
                     /translation="MPEEGFCHSLAGFFKVLGDTTRVRILLALALSEMCVADIACLLG
                     MTQSAISHQLRVLRQARLVKARRMGKISLYSLDDEHVERIVAEGAKHLSHWDRR"
     gene            536..2761
                     /locus_tag="PLM30_02805"
     CDS             536..2761
                     /locus_tag="PLM30_02805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR54132.1"
                     /translation="MSKGSSLKGPNTSATGIGGDPVLKNDRRDQKNRRLVFRLEGLHC
                     RVCALGIERSSAALDWVEGSSVDPVTSKLTLTIRQGSGPPGEPLSKVQEIVDGIEPGV
                     RVLPDDDPVSMGFHKEGSKALKSFSARVLPGLLLWGGALALAPGTSLRTVLFLVAYIL
                     SGWPIVLKALENLLRGRVFDEYFLMTVATMGALVIGEYPEAVAVMLFFRTGEILEESA
                     VEKAGGSITSLVEIIPDKANLLDEGGRAETVTSRSLKPGDRILVKPGERIPVDGIVSE
                     GFSSLDTSSITGESVPRDVAPGDPVLAGFLNNQGTLRVEVSRPLESSAASRILAAIGD
                     AGSKKTRTERLATSFARVYTPSVVILAILIASIPPLIGTGDFETWLYRALIFLVASCP
                     CALLISIPLGIFAGIGSASGKGILIKGGDVLERLPLVDTVFLDKTGTMTTGRFHLAGV
                     YPQRGRSADSLLELAVTAERSSNHPLAEAVKSGWGHRPLPELPLSAIERPGLGIEAKT
                     AESHILAGNRRFMLESGVEPPDPSSSGTMIHVAENGFYAGYLVLADTIKDEAPHAIKE
                     LRGLGVDRIFLLSGDLEGPTVDVEKRLSLDGSFWGLLPEEKVAFLEEMQAASPGGGVS
                     AFVGDGLNDAPVIARAGVGISMGKVGSDITVDNADVVIMNDHLSGLPEALRIARKTRG
                     VIWQNILLSLAVKAAVLALGAFGAATLWEAVFADVGMTLLAVINSSRILRWSRKQRAA
                     A"
     gene            complement(2834..3520)
                     /locus_tag="PLM30_02810"
     CDS             complement(2834..3520)
                     /locus_tag="PLM30_02810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR54133.1"
                     /translation="MEMKENYYSKSLNASRLLDVYQTGIDRVRRYLEAEIEFVKRHLD
                     GVERVLELGAGYGRIMRELAPFAASIVGVEFSKDSVLFGNEYLAPFHNCSFQTLDAHL
                     IDFDSEFDVVLCLQNGLSAMKGDPRNLVERSIRALARGGKAFFSTYSAKFWDHRLEWF
                     KEQSDKGLLGEIDWENTKDGRIVCFDGFKATTFSEEDLDAFGILSGQPYRIEEVDESS
                     LFLVVQKDGA"
     gene            complement(3530..3958)
                     /locus_tag="PLM30_02815"
     CDS             complement(3530..3958)
                     /locus_tag="PLM30_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOR54134.1"
                     /translation="MNSPEVFKGKCRKITEGKVEGEAVVTKDHVNFYLVDPERGTIIE
                     EGHDLEGKKIADKILVFPGDKGSSVVQLDGLYQLAMKNNKPKALVAGELSTVLVSNAI
                     IMEIPLVCDVDEKFYEIIKNGDHIAIDTEEGTISIRAGAC"
     gene            complement(3955..5160)
                     /locus_tag="PLM30_02820"
     CDS             complement(3955..5160)
                     /locus_tag="PLM30_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOR54135.1"
                     /translation="MDLNQKEKDMLEGKYGVGIANAMKILVGIGDAFYAERMVEVSRV
                     HISLSNQEGDLWFANKLVEEGARCCKIATVNPAFNKEYFENVCTFTEQELKVFEDTMS
                     AYRKIGVVPTFQCTPYFVNNVPGFKEIVAFAATGASCFVNSVCGARTNRESSQSALCS
                     AVTGLTPLYGFLLDENRKADTLVEVEAEVKDDFDYQLLGYCLPKKIFTGVPVFHGLSH
                     KPSSEALMNLSTQLNVHGVIPMFHVVGVTPEAATLEDALQGEKPKRKVSITKADLDEA
                     RKTLSHGEGKIDFVVMGCPHLTIQQIKGIAAMLEGKKLKSELWIFTSCLNKELSKRMG
                     LAGIIEGAGGRIVEDSCVDQPIWAHLEGKKGLTESPKCAYYVSRRKMKLIVRSIPDCV
                     NAAIKGEVN"
     gene            complement(5385..6347)
                     /gene="dctP"
                     /locus_tag="PLM30_02825"
     CDS             complement(5385..6347)
                     /gene="dctP"
                     /locus_tag="PLM30_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOR54136.1"
                     /translation="MAEAAAPEVFKWRLQAVGPRGSTETDVIERFADQVKQASNGRLE
                     IKVYGADEIVPTFETFDAVSQGLLEMHCNDPSYWVGKTNQCSGIITTPFLFKNALSQK
                     IFLENWGGLEILRKVYAKHNIYLINQFGETPGTIWTKFPFKGLEDFKGKKIRGHGLWS
                     EALKRVGVSSVTMPGGEVYEAMERGVIDGLISANVAWCFDYGFHEVSNYVYAGDGINI
                     ANLEFAVNMDAWNKLPDDLKAIVSVCAREAEVAMLAINQDADQVKLHEAVEKWGVKTQ
                     PFDSASIGKMRAAMMGAIEEYAKADESYAELLKVLKKYLEVTGD"
     gene            6869..>7242
                     /locus_tag="PLM30_02830"
     CDS             6869..>7242
                     /locus_tag="PLM30_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOR54137.1"
                     /translation="MKEVNKLNQEAMVIGPDMELPPYPEFKEGIRRAPDRGWTLNEAE
                     TELALKNALRYVPEKFHEVLAPEFMEELTTRGRIYAYRFRPAGRLYGKPIQEYKGRCM
                     AGKAFQVMIDNNLDFDVALYPY"
BASE COUNT         1533 a         2112 c         1942 g         1655 t
ORIGIN      
        1 cttgaacaac cccgttcggg gcgtcggcgg gtgggaggct cggctcctct tcatcgttat
       61 ccttgtccgc taggacttga caagggattt taccatgaaa taccgggccc gcgcaagggc
      121 aatggggtca tgcagacctg ctcggctcat ttttcacgga ctcgcttgac aaaagggcgg
      181 gtttagatta tatgaatagt tgctcatata ttcatgaatg aggtgaatcc gttggaacgg
      241 agggtgaata tacccgaggc catccacggg gagaccgtga gggccgcgaa gaacaggatg
      301 cctgaagaag gtttctgtca ctccttggcg ggcttcttca aggtcctcgg tgatacgacc
      361 agggtgagga tactcctggc gctcgccctt tcagaaatgt gcgtggccga catagcctgc
      421 ttgcttggaa tgacccaatc ggccatctcc catcaactaa gagtcctcag gcaggcacgg
      481 ctggtcaagg ccagaaggat gggaaagata tccctctact ccctcgacga tgagcatgtc
      541 gaaaggatcg tcgctgaagg ggccaaacac ctcagccact gggatcggag gtgaccctgt
      601 cctgaaaaat gaccgacggg accaaaagaa ccgccgcctg gttttccgcc tggaaggcct
      661 ccattgccgg gtctgcgccc tgggtattga gagatcctca gcggcgctgg attgggtcga
      721 aggttcttcc gtcgaccccg tcacttcaaa gctgaccctc accatccgcc agggatcggg
      781 tcccccgggg gaaccccttt cgaaggtcca ggagatcgtc gacgggatcg aacccggcgt
      841 cagggtcttg cccgatgatg atccggtgag tatgggtttt cacaaggaag ggagcaaggc
      901 cttgaagtcc ttttcggcca gggtcctccc gggacttctc ctatggggcg gtgcgcttgc
      961 gctggcaccg gggacatccc tgcggacagt cctcttcctg gtcgcctaca tcctgtccgg
     1021 gtggcccatt gtcctgaagg ccctcgaaaa cttgctgcgc ggcagggttt ttgatgaata
     1081 cttcctcatg accgtagcta ccatgggcgc cctggtcata ggggagtatc ccgaggcggt
     1141 cgcggtaatg ctctttttca ggaccggcga gatccttgaa gaaagcgccg tcgaaaaggc
     1201 cggcgggtcg atcacttccc tggtggagat catccctgac aaggctaacc tgctcgacga
     1261 aggcggcagg gcggaaacgg taacttcccg gtccctgaaa cccggtgaca ggatcctcgt
     1321 gaaaccgggc gagaggatac ccgtcgatgg tatcgtatca gaggggtttt ccagcctgga
     1381 cacatcatcg atcaccgggg agtcggtccc ccgtgatgtt gcgccggggg atcccgtcct
     1441 ggccgggttc ctgaacaacc agggcactct tcgcgtcgag gtctcgagac cgcttgagtc
     1501 ctcggcggct tcaaggatcc tggcggccat cggcgacgcg ggttcaaaaa agacacggac
     1561 cgaacgactc gccacgtcct ttgccagggt ctacactcct tccgtggtga tcctggcgat
     1621 cttgatcgct tcgatcccgc cacttatagg gacaggcgac ttcgagacct ggctctacag
     1681 ggctttgatc ttccttgtcg cctcctgtcc ttgcgctctc ctcatttcga tcccgctggg
     1741 cattttcgcg ggcattggct ccgcctccgg gaaggggatc ctgatcaagg ggggcgatgt
     1801 cctcgaaagg ctgcccctgg ttgacacggt cttcctcgac aaaaccggga ccatgaccac
     1861 cggccgcttc cacctggccg gggtctaccc ccagaggggg aggtcggccg actcccttct
     1921 cgagctggcc gtcaccgccg agaggagttc aaatcacccc ctcgccgaag ccgtaaagtc
     1981 cgggtggggg catcgaccct tgccggaact ccctctttcg gccatcgagc gtccagggct
     2041 cgggatcgag gcgaaaacgg ccgagtccca tatcctcgcc ggaaacagga ggttcatgct
     2101 tgaaagcggg gtcgaaccgc ccgacccctc ctcgtcgggg acgatgatcc acgtggcgga
     2161 aaacggcttt tacgcgggat acctggtcct ggctgatacc atcaaggacg aggcgcccca
     2221 cgcgatcaag gaattgaggg ggcttggcgt tgatcgtatt ttcctcctct cgggcgacct
     2281 ggagggcccc accgtggatg tggagaaacg actatccttg gacgggtcct tctgggggct
     2341 cctgcccgag gagaaggtgg cgttccttga ggaaatgcag gccgccagcc cgggaggggg
     2401 cgtcagcgcg ttcgtgggcg acggtcttaa cgatgccccc gtcatcgcca gggccggcgt
     2461 gggcatctcc atggggaagg taggttccga cataaccgtc gacaacgccg acgtggtgat
     2521 catgaacgac catctgtcgg gcctgccgga agccttgagg atagcccgaa aaacaagggg
     2581 agtgatctgg cagaacatcc tcttgtccct cgcggtgaaa gcggccgtcc tggcccttgg
     2641 cgccttcggc gccgcgaccc tttgggaagc cgtcttcgcc gacgtgggga tgaccctcct
     2701 ggccgtcatc aactcttcaa ggatcctgcg atggtccagg aaacaacggg ccgctgctta
     2761 agaagccgcg gcccgttccc tccgggcggt cattcagacc tggtgctgtc ggccgacatc
     2821 agggccaata gtgtcaggct ccgtcttttt gaaccacgag gaaaaggctc gactcgtcca
     2881 cttcttcaat gcgatagggt tgaccgctca ggatgccaaa ggcatcgagg tcctcttccg
     2941 agaaggtggt cgctttgaaa ccatcaaagc acactatcct gccgtctttc gtgttctccc
     3001 agtcgatctc cccaagcaag cccttgtcgg actgctcttt gaaccattcc aggcgatgat
     3061 cccagaattt ggcactataa gtgctgaaga aggcttttcc cccacgggca agggccctga
     3121 tgctcctttc aaccaggttc cgtggatcgc ctttcatggc ggacaaaccg ttctggaggc
     3181 ataggaccac gtcaaattca gaatcgaaat cgataaggtg ggcatccaag gtttggaaac
     3241 tgcaattgtg gaagggcgcg aggtattcgt tcccgaaaag gacggagtcc tttgagaact
     3301 ccacacccac gatcgaagct gcgaagggtg ccaattctct cataatgcgt ccgtaaccgg
     3361 ccccaagctc taggacacgc tccaccccgt cgaggtgcct cttgacgaat tcgatctcgg
     3421 cttcgaggta ccgcctgacc cggtcgatcc ccgtctggta tacatcaagg agccgtgacg
     3481 cgttgaggct tttggaataa taattctcct tcatctccat gaccactttt taacacgccc
     3541 ctgctcgtat cgatatcgtc ccttcctccg tgtctatcgc gatgtggtct ccgttcttta
     3601 tgatctcgta aaacttttca tccacatcac aaaccagcgg tatttccatg ataatggcat
     3661 tgctgaccaa aacggtgctc aattctccag ctacgagggc cttgggcttg ttattcttca
     3721 tggccagctg gtacagcccg tccaattgga cgacggagct gcccttatct cccgggaaaa
     3781 cgaggatctt atccgcgatc tttttgcctt ccaggtcgtg gccctcctcg atgatcgtcc
     3841 ctctctccgg gtcaacgaga taaaagttta cgtggtcctt cgtgaccacc gcttcccctt
     3901 ctaccttccc ctcggtgatc ttccggcatt tgcctttaaa aacttcaggg ctgttcaatt
     3961 cacttccccc ttaatcgctg cgtttacaca gtcgggaata ctcctgacga tcaacttcat
     4021 tttccttctg gatacgtagt aagcacattt cggggattcg gttaggccct tcttgccttc
     4081 caggtgtgcc cagatgggct ggtccacgca actgtcctct acaatgcggc ccccggctcc
     4141 ctcgataatg ccggcgagcc ccatcctctt ggaaagctcc ttgttcaggc aggacgtgaa
     4201 tatccaaagt tcggacttaa gctttttgcc ctccagcatc gcggcaatgc cctttatctg
     4261 ctgaatggtc agatgggggc aacccattac cacaaaatcg atcttgccct ccccgtggga
     4321 aagggtcttc cttgcttcgt caaggtcggc tttcgtaatg gatacctttc ttttgggttt
     4381 ctctccctgc aaggcatcct ctagggtggc ggcctcgggg gtgaccccca cgacgtggaa
     4441 catggggata acaccgtgga catttaactg ggtgctgaga ttcataaggg cctcgctcga
     4501 gggtttatga ctcagcccgt gaaaaacggg gacaccggta aagatctttt tgggcaagca
     4561 gtatccgagg agctggtaat cgaaatcgtc tttgacttcg gcttcgacct ccaccaaagt
     4621 gtcggccttc ctgttttcat ccagaaggaa cccgtacaag ggggtaagtc cggtcaccgc
     4681 gctgcaaagg gcgctctggg aggattcccg gttagtcctt gccccacaga cggaattgac
     4741 gaaacaggaa gcaccggtgg cagcgaaggc gactatttcc ttgaacccgg gaacattatt
     4801 cacgaagtag ggcgtgcatt gaaaggtggg gacgaccccg atcttcctat aggcactcat
     4861 ggtatcttca aagaccttca gttcctgctc agtgaaggta cagacgttct caaaatattc
     4921 tttgttaaag gcggggttca ccgtagcgat cttgcaacac ctcgccccct cctcaaccag
     4981 tttattggcg aaccagaggt cgccttcctg gttgctcagc gagatatgga cccgggagac
     5041 ctccaccatg cgttccgcat agaaggcgtc ccctattcct accagtatct tcatggcgtt
     5101 cgcaataccg acgccatact ttccttccag catatccttt tctttctgat tcagatccat
     5161 tagtccttcc tcctggcctt tccgagaatt ggcgaaaaag acgagcttcg gaacctgaca
     5221 agggacatcc ggcaccgtcc aaacaaaagg tataagggac cccctttggg gaatccccta
     5281 taccttcttc gttgtcgggt caggtccttt gcgaatctcc catcgggtcg tacaaaatga
     5341 ccctcttttt agatctttag gttacctctt gatcgaggta cctcctaatc gccggtaacc
     5401 tccaggtact ttttcaacac cttcagcaat tcggcgtagg attcatcggc cttggcgtac
     5461 tcctcgatgg cgcccatcat ggccgccctc atcttgccta tgctcgcgga atcaaagggc
     5521 tgcgttttca caccccattt ttcgaccgct tcatggagct ttacctggtc cgcgtcctga
     5581 ttgatcgcga gcatcgcgac ctccgcctct ctcgcgcaga ctgaaacgat ggccttcagg
     5641 tcgtcgggca gcttgttcca ggcatccatg ttcacggcga attccaggtt ggcgatattg
     5701 atgccgtcac cggcatagac gtagttcgaa acctcgtgga agccgtagtc aaagcaccag
     5761 gccacattcg cggagataag cccatcgatc acgcctcttt ccatggcttc atagacttcg
     5821 ccgccgggca tggtcactga ggatacgccg acccttttca gagcctcaga ccacaggccg
     5881 tgacccctga tcttcttgcc tttgaaatcc tccagccctt tgaaagggaa ctttgtccag
     5941 atcgtcccgg gggtctcccc gaactggttg ataaggtaaa tattatgctt ggcgtatacc
     6001 ttgcggagta tctcaagacc gccccagttt tccaggaaga tcttttggct cagagcgttc
     6061 ttgaagagga agggtgtcgt aatgatcccc gaacactggt tggtctttcc cacccaataa
     6121 ctggggtcat tgcagtgcat ttcgagcaac ccctgcgaga cggcatcaaa ggtttcgaaa
     6181 gtcggtacga tctcatcggc gccgtaaacc ttgatttcca gtctcccatt gctggcctgt
     6241 ttcacttgat cggcaaatct ttcaatgacg tcggtttcag tgcttccccg aggtcccacg
     6301 gcctgcaggc gccacttgaa cacttccggg gctgcggcct cagccaagga ggaaccgaag
     6361 aggaaaacaa acgcgcacaa agcgagaaga atcccaaaaa cgataccttt cgtgcctttc
     6421 actttaaccc ctcccaagat cgttttctcc ttcgaaagac cgttacctgt caggaactgg
     6481 atttttcgat atcacccccc ccgcccgata acgataacat tgacgcgaaa tacttcaaac
     6541 aaaacccgag actgggccca ggacaactag gacacaattc cacactctct tcccgcgagg
     6601 gtaaatggag acctttggtc gacaaaagac tatttttgta agacaatatc ttaacggaga
     6661 ttctttgtca aggcacaacg gcccttcagc gtggccgtcc acggtttttt gccggtgtta
     6721 gaaagaagga agagaccctt tgtccaattc ttttttgaaa ggagggttat aattgaaacg
     6781 gccaagggaa aagcctgatc agcggagccg ttggtgaacc gtcgcagccg atctgtgtcc
     6841 cagtcggatt tcgaaaggag gtaacaaaat gaaagaagtg aacaagctga accaggaagc
     6901 tatggtgatc ggtcctgaca tggagcttcc accttacccc gaattcaagg aagggatcag
     6961 gcgcgccccc gaccggggtt ggacccttaa cgaggctgaa accgaactgg ccctcaagaa
     7021 cgccctacgg tacgtccccg agaaattcca cgaggttctg gccccagaat tcatggagga
     7081 attgaccacc cggggaagga tctatgccta ccggttccgc cccgcaggaa ggctctatgg
     7141 gaaacccatc caggaataca agggcaggtg catggccggc aaggctttcc aggtcatgat
     7201 cgacaacaac ctcgactttg acgtggccct ttacccctat ga
//
LOCUS       DAOSRG010000044         6821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_10324_length_6821_cov_6.044635, whole genome shotgun sequence.
ACCESSION   DAOSRG010000044 DAOSRG010000000
VERSION     DAOSRG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_6821_cov_6.044635"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLM30_02835"
     CDS             complement(<1..331)
                     /locus_tag="PLM30_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR54138.1"
                     /translation="MLVCKGTGCTASGSPSVYEALKKEIERRGLDQEVALVETGCHGM
                     CEMGPIVVIYPEGSLYCRVTADDVPEIVEEHLYKGRLVQRLLYTTPDRKFKVPHYRDI
                     PFYSKQLR"
     gene            complement(367..735)
                     /locus_tag="PLM30_02840"
     CDS             complement(367..735)
                     /locus_tag="PLM30_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR54139.1"
                     /translation="MKVTSLEDLRKIKENAKDLTSARSRGATRVIIGMGTCGIAAGAR
                     EVMTAILEELDKRGLKDVAVETTGCIGMCQKEPLVDIIRPGEPRTTYGEVSPADAVRI
                     IAEHLVNGHIVEDKVIGQTD"
     gene            complement(759..1229)
                     /gene="nuoE"
                     /locus_tag="PLM30_02845"
     CDS             complement(759..1229)
                     /gene="nuoE"
                     /locus_tag="PLM30_02845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOR54140.1"
                     /translation="MNPKKQEVAERTRQIVAPWKGARGGTIPVLQEVQKAFGFLPEEA
                     LMEISRELKIPPSELYGVATFYAQFHLKPRGRHIVRVCRGTACHVRGSLAILDKIKTV
                     LDVEENGTTDDRRYTLEPVACLGACGLAPVMMVDQETHGRLTPDKVMEVLGSYE"
     gene            1603..>2335
                     /locus_tag="PLM30_02850"
     CDS             1603..>2335
                     /locus_tag="PLM30_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54141.1"
                     /translation="MKKAEIEFDVVVLLVAALTMLLTGALLFPVARGLLPYYENGVYG
                     LFLFIFALQMVTLGRTPFGDAPRSKALIAVGVVVASLGMITCFIPEVLSRVPQILLSI
                     SFGLGGIALLLQMILSPDRFPTWRRYGGVFRHLIAGCAAVYALSALIGLLVARKDLLS
                     TPMTAVVVLAMGFSLTYLAVTLQKIYNTYPEAVQEPKGELDLPIERAMILLTGVFMVI
                     LGVLLVPVNLGKLPFSGSAQLGLLMV"
     assembly_gap    2336..2435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2436..2942
                     /locus_tag="PLM30_02855"
     CDS             <2436..2942
                     /locus_tag="PLM30_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54142.1"
                     /translation="NLGKLPFSGSAQLGLLMVIMAIQILATGASPIGSFPRTWLMIII
                     GLVFVALGATSCIIPWVLVAPLTLLIGCTNILGGVLKLKEILVPIIKGPRGAGPVPPV
                     LVRLNLVQVAMNLVSITFGASMLIHDLLPGMVIGVVLAANGGLLLYLMHILYELDRLQ
                     KTMSQPAS"
     regulatory      3035..3156
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3212..4006
                     /locus_tag="PLM30_02860"
     CDS             3212..4006
                     /locus_tag="PLM30_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54143.1"
                     /translation="MDRKFRERWFMLLMGSFLVVYIIWPLAGMFFRADWSIVASTTRD
                     KVVIGALWRSVWTAAAATGVITLFGTPLAYFLARQEFRGKSVLEAVIDVPIMVPHTVA
                     GIAVLMTFSPKAPIGAMLVRAGFSPVNSHLAIILACMFVSIPFYVDAARDAFYGVSPR
                     LENVSRTLGASFFHVFFRISLPLAKRGLVSGLIMSWARGVSEFGAIVIMAYHPMVAPT
                     LIYDRFATFGLKFSTPIAVQLILVCLGMFVVLRVLVSGKRKEVIGG"
     gene            4003..5046
                     /locus_tag="PLM30_02865"
     CDS             4003..5046
                     /locus_tag="PLM30_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54144.1"
                     /translation="MIRLAGLSIDLGEFVLDSFSLSVDEGEFFMVVGPSGAGKTVLLE
                     AIAGLRQPSGGKILVAGRDVTDLPPEKRNVALVYQDYALFPHLSVEENIRWGLRFVPR
                     PDGPHVEGLVRLLRLEHLLERQPETLSGGEQQRVALARALAVKPSVILLDEPISALDP
                     RFREDLQDYLSRINREGMTIMMVTHDFNEVLSLGHRVAVIVEGSLQQVGEVKTVFREP
                     ANRLVADFVGMKNLFPSSVKGRRASLEGGLSLVLDGQKAGGECLIGIRPEDVMIGQDL
                     AEGTENVFGGRVVSIVPRGMAFEVVFEIGGIRLTGRMLSSALVREDVRPGVDCRVGFY
                     PDAIHVFEGSHKI"
     gene            5062..6015
                     /gene="wtpA"
                     /locus_tag="PLM30_02870"
     CDS             5062..6015
                     /gene="wtpA"
                     /locus_tag="PLM30_02870"
                     /inference="COORDINATES: protein motif:HMM:NF003196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HOR54145.1"
                     /translation="MSRFTRITVLAMALVMVFAATAAFADEKVIVFHAGSLSTPMEKV
                     EKIFEEAHPGVDVQREAGGSAALARKIIDLGGACDVFMSADYMVIEKILRPAEADWNI
                     LFSSNAIVLMYSPQSKYKDEINKDNWTEVLMRPDVRWGHSEPDQDPCGYRSLMVLQLA
                     ETFYNDKDLYARALKHPERAVRPKAIDLVAMVQSGAMDYAFEYKSVAMQHGLEYLDLP
                     DEVNLKNPDFADQYAAATVELAGSEPGKTILAKGEPIVFALTIPRNAPNPKRALELVE
                     LILAKDGGLKILEEMGQDTVGPQTYTPGENIPAELQPLMVK"
     gene            complement(6101..6310)
                     /locus_tag="PLM30_02875"
     CDS             complement(6101..6310)
                     /locus_tag="PLM30_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOR54146.1"
                     /translation="MKLSARNALKGKVKKVTAGVVNTEVVVELPGGDEVVSMISKESA
                     EKLGLKPGKDVYAVIKATNVMIGID"
     gene            6703..>6821
                     /locus_tag="PLM30_02880"
     CDS             6703..>6821
                     /locus_tag="PLM30_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54147.1"
                     /translation="MEERAYRSIIGLILSGKYRPGNFLLEVDLASQLGVSRTPV"
BASE COUNT         1215 a         2065 c         2030 g         1411 t
ORIGIN      
        1 tgcggagctg cttgctgtag aagggtatgt cccggtaatg ggggaccttg aatttcctgt
       61 cgggcgtggt gtagaggagc cgctggacca agcgcccctt gtaaaggtgc tcctcgacga
      121 tctccgggac gtcgtcggcg gtcaccctgc agtagaggga gccctcgggg tagatcacca
      181 cgatgggtcc catctcgcac atcccgtgac acccggtctc gaccagggcc acttcctggt
      241 cgagtcccct tcgctctatc tccttcttaa gagcctcgta caccgaagga gacccgctgg
      301 cggtgcagcc ggtccctttg cagaccagca cgtgggcgcg gtaaaaggcc atgaccgccg
      361 agctccctag tcggtctgcc cgatgacctt gtcctcgacg atatggccgt tgacaaggtg
      421 ttcggcgatt atccttacgg cgtcggctgg gctgacttcg ccgtaggtcg tccgcggttc
      481 gccgggcctg atgatgtcca ccaggggttc cttctggcac atgccgatgc agccggtggt
      541 ctcgacggcc acatccttaa ggcccctctt gtccagttcc tccaggatcg ccgtcatgac
      601 ctcccgggcc cctgccgcga taccgcaggt gcccatgccg atgatcaccc tggtggcacc
      661 ccgtgaacga gcggacgtga ggtccttggc attctccttg atcttcctga gatcttccag
      721 gctagtcact ttcaccggtg ttcacctcca gcgtggtcct attcgtagga acccagtact
      781 tccataacct tgtccggcgt gagccgtcca tgggtctcct ggtccaccat catcacgggg
      841 gcgagcccgc aggccccgag gcaggcgacg ggctcaaggg tgtaccgccg gtcatcggtg
      901 gtgccgttct cctcgacgtc aaggaccgtc ttgatcttgt cgaggatggc gaggctgccc
      961 ctgacgtggc atgccgtccc ccggcagacc ctgacgatgt gccgccctcg tggtttcagg
     1021 tggaactggg cgtagaaggt ggccactccg tagagctccg agggcgggat cttcaattcc
     1081 cgggagatct ccatcagggc ctcctcgggg agaaagccga aggccttctg gacctcctgg
     1141 aggacgggga tcgttcctcc ccgcgcgcct ttccaggggg ccacgatctg tctcgtcctt
     1201 tccgccactt cctgcttttt cgggttcact ccctatctcc ctccttcggg tctcgaagcc
     1261 attatgccca gactgtcgat gaacaggtcc gtatccccgg tacgggatct cttggggccg
     1321 ctttgggata cattgaaacc gatagtgaaa gagaacgcaa ccgggaaaag ggccggtctc
     1381 ccagggaaaa aatgcacctt gaggcccatt atcgaccatg ggttgaaaag aagtcaaaga
     1441 aagacaggac ccggaccgat acaccatggc ctcgaacccc tgatcgcagg tccctgaaaa
     1501 aggacagcgg gataatgtat catgacttca acagagtgtt gccacgggcg attgatacca
     1561 gtttttcacg aaggggatga tgtcgtggtt tcgtggggga tcatgaaaaa agcggagatc
     1621 gagttcgatg tggtggtgct ccttgtcgca gccttgacga tgctcttgac gggcgccttg
     1681 ctcttcccgg tggctcgggg tcttctcccc tactacgaga acggggttta cgggcttttt
     1741 ctttttatct tcgccctcca gatggtcacc ctggggcgga cccccttcgg cgatgccccc
     1801 cggtcaaagg cgctcatcgc cgtcggggta gtggtggcct ccctggggat gatcacctgc
     1861 ttcatccccg aggtcttaag ccgcgttccg caaatccttc tgtccattag tttcggcctg
     1921 ggcggcatcg cccttcttct gcagatgatc ctttccccgg acaggttccc gacgtggagg
     1981 cggtacgggg gggtcttccg acacctcatc gcgggctgcg ctgcggtcta cgccctgtcg
     2041 gccctgatag ggctcctggt ggcgcggaaa gatttgctct ccacaccgat gacagccgtc
     2101 gtggtcctgg cgatgggttt ttccctcacc tatctcgccg taaccctgca gaagatctat
     2161 aacacctacc ccgaagcagt ccaggagccg aagggtgagc tggacctgcc cattgagagg
     2221 gccatgatcc tcctgaccgg tgttttcatg gtcatcctcg gtgtgctcct cgtgcctgtt
     2281 aacctcggga aacttccctt ttccgggagc gcccagctgg gcctgctgat ggtgannnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacct cgggaaactt cccttttccg
     2461 ggagcgccca gctgggcctg ctgatggtga tcatggccat ccagatactt gccacggggg
     2521 cttcccccat tggttccttc ccccggacat ggctcatgat cattatcggc ctcgtctttg
     2581 tcgctctcgg ggcgacgtcg tgcatcatcc cctgggtcct ggtcgccccg ctgacgctcc
     2641 tcatcggctg cacgaacatc ctgggagggg tcctgaaact caaggagatc ctggtgccca
     2701 tcatcaaggg tccccggggg gccgggcccg tccccccggt cctggtcagg ctgaacctcg
     2761 tccaggtggc catgaatctg gtatccatca ccttcggcgc ttccatgctc atccacgacc
     2821 tgctccccgg catggtcatc ggtgtcgtcc ttgccgccaa cggagggctc ctgctttacc
     2881 tgatgcatat attgtatgag ctggacaggt tgcagaagac gatgtcgcag ccggcctcct
     2941 gagggacttc gaagccgaga aatgagcggc aaggcgatct agcgggttat cattgtaacg
     3001 gtaagggtaa tgtgttagta tccttggtgc ccggtcgccg ggcgtcctcc gagtcgcgtc
     3061 cccttgcggg ctttcgccca tggggagcgg ccgatgggtt ttcctggaaa cgggaaagcc
     3121 tcccgcgttt ggaaaggagg agcggtcagt tgttatacga ggccgcctcc tgtacctggg
     3181 gggcggcctt tttttgcctg ggggtgactt tttggaccgc aagttcaggg aacgctggtt
     3241 catgctcctc atggggtcct tcctggtggt ctacatcatc tggcccctgg cgggcatgtt
     3301 cttcagggcc gactggagca tcgtcgcctc gaccacccgg gacaaggtgg tcataggggc
     3361 cctctggagg agcgtctgga ccgccgcggc cgccacaggg gtgatcaccc tcttcggtac
     3421 ccccctcgcc tacttcctgg ccaggcagga gttcaggggc aaatcggtgc tcgaggccgt
     3481 gattgacgtc cccataatgg tcccccatac cgtggcgggc atcgccgtcc tcatgacctt
     3541 ttcccccaag gcccccatcg gagcgatgct ggtccgggcg gggttttcgc ccgtcaacag
     3601 ccacctggcc ataatcctgg cctgcatgtt tgtctcgatc cccttttacg tggacgcggc
     3661 aagggacgcc ttctacggcg tgtcacccag gctggagaac gtctcccgca ccctgggagc
     3721 ctccttcttc cacgtgtttt tccgcatatc ccttcccctg gcgaaaaggg gtctcgtctc
     3781 cggcctgatc atgagctggg ccaggggggt gagcgagttc ggcgccatcg tcatcatggc
     3841 ctaccacccg atggtggccc ccaccctgat ctacgaccgt ttcgccacct tcgggctcaa
     3901 gttctccacg ccgatcgcgg tacagctcat cctggtctgc ctggggatgt tcgtcgtact
     3961 gcgggtcctg gtatcgggaa agcgcaagga ggtcatcggc ggttgataag actggcaggc
     4021 ctctcgatcg acctgggcga gttcgtgctg gactccttct ccctctcggt ggatgagggg
     4081 gagttcttca tggtggtggg gcccagcggc gcggggaaga cggtcctcct tgaggccata
     4141 gccggtttga ggcagccctc gggcggaaag atcctcgtcg ccggccggga cgtcaccgac
     4201 ctgccgccgg agaagaggaa cgtggccctg gtttaccagg attacgccct gttcccccat
     4261 ttgagcgtgg aagagaacat ccgctggggg ctgaggttcg tcccaaggcc ggacggcccc
     4321 cacgtggaag gtctcgtgag gctgctgagg ctcgaacatc tcctcgaaag acagccggag
     4381 actctctcgg gcggtgagca gcagcgcgtg gcgctggcca gggcgctggc ggtcaaaccc
     4441 tcggtcatcc tccttgacga gccgatatcg gcgctggacc cgcgcttcag ggaggacctg
     4501 caggactacc tctcccggat caaccgagag ggaatgacca tcatgatggt gacccacgat
     4561 ttcaacgagg tcctttccct ggggcacagg gtcgccgtga tagtcgaggg gtccctccag
     4621 caggtgggtg aagtcaagac cgttttccgc gaacccgcca acaggctggt ggccgatttc
     4681 gtgggcatga agaacctctt cccgtcgagc gtcaagggga ggagggccag ccttgaggga
     4741 gggctttccc tcgtcctgga cggacagaaa gccggcggcg agtgcctcat agggatacgc
     4801 cccgaggatg tgatgatcgg ccaggacctg gcggagggga ccgagaacgt cttcggcggc
     4861 cgcgtggtga gcatcgtacc cagggggatg gctttcgagg tggtcttcga gatcggcggc
     4921 atcaggctga cgggcaggat gctctcctcg gccctggtga gggaggatgt ccggccgggg
     4981 gtggattgca gggtggggtt ctaccctgat gcgatacacg tgttcgaagg gagtcacaaa
     5041 atttaacgga ggtgttcagt aatgagcagg ttcacaagga tcacggttct ggcgatggcc
     5101 ctcgtcatgg tcttcgccgc gacggcggcc ttcgccgacg agaaggtcat cgtcttccac
     5161 gccggcagcc tgtcgacccc catggagaag gtcgagaaga tcttcgaaga ggcccacccc
     5221 ggtgtcgatg tccagcgcga ggccggtggg agcgcagccc tggccaggaa gataatcgac
     5281 ctcgggggtg cctgcgacgt cttcatgtcg gcggactaca tggtcatcga gaagatcctg
     5341 aggccggccg aggccgactg gaacattctc ttctccagca acgccatcgt actcatgtac
     5401 agcccccagt ccaagtacaa ggatgagatc aacaaggaca actggaccga agtgctcatg
     5461 cgccccgatg tccgctgggg gcactccgag ccggaccagg acccctgcgg ttaccgcagc
     5521 ctcatggtcc tgcagctggc cgagaccttc tacaacgaca aggacctcta cgccagggcg
     5581 cttaagcacc ccgagagggc ggtgcgcccg aaggccatcg acctcgtggc catggtgcag
     5641 agcggcgcca tggactacgc ctttgagtac aagtccgtgg cgatgcagca cggcctcgag
     5701 tacctggacc tccccgacga ggtcaacctg aagaaccccg acttcgccga ccagtatgcc
     5761 gcagccacgg tggaactggc gggcagcgaa ccgggcaaga cgatcctcgc caagggtgag
     5821 cccatcgtct tcgcgctgac gatcccccgg aacgcgccca acccgaagcg cgccctggaa
     5881 ctggtggaac tcatccttgc caaggacggc gggctgaaga tcctcgagga gatggggcag
     5941 gacaccgtcg gtccccagac ctacacgccg ggagagaata tccccgccga gctccagccg
     6001 ctgatggtga aatagcacgt caagaacaaa aaagggcccc gactcatgtc ggggcccctt
     6061 ttgtatcaaa actaacgttc gatgggtaac tcccttaccg tcagtcgatg ccgatcatga
     6121 cgttggtggc cttgatgacg gcgtagacgt ccttcccggg tttcagcccc aacttctcgg
     6181 ccgactcctt ggagatcatg gagaccacct catcgccgcc ggggagttcc acgacgacct
     6241 cggtgttcac cacgccggcc gtaaccttct tgaccttgcc ctttaacgcg ttgcgggcgc
     6301 tgagtttcaa gatatccacc tcccgagata tacctacata gggtttatct tttattcaca
     6361 taataatcct aacacagggt cccatcgtaa tcaagacctt tacgaagcgg gcccgatcat
     6421 cggatcttcg ccatggcgcc tcccactttt gacggtcagt aaggtggaat aatgaacagg
     6481 ctttgataaa ctccaccacg ctaagggaaa gcatcccact gggtcaaacg ccctttccaa
     6541 taggccgacg ttgtatcggc cccggctcat tagcattatt tttcaaagaa agacgtgcgt
     6601 gggaccggtg tttgacaagg ggcggcccct ttcctaaact gatgaataca cctgtgatgt
     6661 atccatctgc ttgcccggga ggcccaacaa gtgaaagaca acgtggaaga aagagcctat
     6721 agatctatca ttggccttat cctttcaggg aagtacaggc ccggaaattt cctcctcgag
     6781 gtggacctcg cctctcagct cggcgtgagc aggaccccgg t
//
LOCUS       DAOSRG010000045         6560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_10813_length_6560_cov_8.093313, whole genome shotgun sequence.
ACCESSION   DAOSRG010000045 DAOSRG010000000
VERSION     DAOSRG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10813_length_6560_cov_8.093313"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLM30_02885"
     CDS             complement(<1..242)
                     /locus_tag="PLM30_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOR54148.1"
                     /translation="MPAEDSRDLYAILGVSRDASQEEIKKAYRKLVRKHHPDANPGDP
                     GAEERFKKINLAYEVLQDPQKRAAYDRYGTIGDAPPG"
     gene            complement(256..>1836)
                     /gene="dnaK"
                     /locus_tag="PLM30_02890"
     CDS             complement(256..>1836)
                     /gene="dnaK"
                     /locus_tag="PLM30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOR54149.1"
                     /translation="GASQTPQQISAMILQKLRRDAEEYLGEKITQAVITVPAYFTDAQ
                     RQATKDAGKIAGLEVLRIINEPTAAALAYGMDKGGENKILVFDLGGGTFDVSVLDVGI
                     EDGVIEVLATSGDNHLGGDDWDQKIVDLMVSEFKKNEGIDLSRDKMALQRLREAAEKA
                     KVELSSMTETSISLPFITADSTGPKHLELSLTRARFEEITRSLLEKVVEPTRRAMNDS
                     GLTREEIHKVLLVGGATRMPMVQRKIVELLGKEPTKGINPDECVAVGAAIQAAILTGE
                     HKDIILVDVTPLSLGIETLGGVFTKILDRNTAIPASKSQVFSTASDNQPQVEIHVLQG
                     ERSMAADNVTLGKFFLDGIPPAPRGMPQIEVTFDIDVNGILNVSARDKGTSREQHVTI
                     QSSRLTEAEIERMRRTAEENLESDRKRKELVDARNEGDSLIYSTEKTLSDLGDRVTDA
                     EKTRVREEIDHLRGKLAGEDPGEIRQACEILTKSLHEMSARLYDQAAGQQEAHQGSSG
                     PGTDGDTVDAEFEENGKP"
     assembly_gap    1837..1936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1937..2322)
                     /gene="grpE"
                     /locus_tag="PLM30_02895"
     CDS             complement(<1937..2322)
                     /gene="grpE"
                     /locus_tag="PLM30_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOR54150.1"
                     /translation="MIRPEGKRPQENPEDGSRGDSGEGGLEATIEKEPHEEGSADNEG
                     LLSELGSAKALVERLSEENKALGDAIARARADFFNYRQRVEREQERLRSMAGEDAAIA
                     LIPVLDNLDRALSGNRSDIESYVKGVG"
     gene            complement(2319..3371)
                     /gene="hrcA"
                     /locus_tag="PLM30_02900"
     CDS             complement(2319..3371)
                     /gene="hrcA"
                     /locus_tag="PLM30_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOR54151.1"
                     /translation="MLSERQLEVVLAVVYEYIKTGEPVGSRTIARKFLKGRSAATIRN
                     EMADLEESEYLYQPHTSAGRIPTPKAYRVYVDAILRRQRSFPSESEGWIKELRERRKD
                     VEEALIYATRLLGRITSYIGLAALGPEREPVLERVEFLRMGHSTVLILLVLKGGLIHH
                     KISTLPCDMTQEALDELSRRVSTVAVGRSWGEVRGALQAYVEDALERYAESCRSALNE
                     MDGLLLEQPFQFFTGGTHNVLGLPDFSDLGRLQTIMALLEEDESIGMLVRSCSTEKGL
                     RVTIGDENPELNMPDCSILMASTIAGGRKAVVGLIGPMRMDYERSISVLEGVLGTIAG
                     KGENGENGESAKEVHR"
     assembly_gap    3655..3754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3969..>4867
                     /gene="folP"
                     /locus_tag="PLM30_02905"
     CDS             3969..>4867
                     /gene="folP"
                     /locus_tag="PLM30_02905"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOR54152.1"
                     /translation="MSLYRIEIESRKDLADLVRVTGADERSFYFFERKRRMLAFFIPE
                     VDFRAANALKQELLSRGGDAIVHRGAIAGSVPTSSVVILGTLKTLEDLSVKLRSMPYW
                     GLDVVKDQLLRALSSLGVKRWNLKVPGRDDLELGGHTKVMGAINITTDSFYPGSRVKG
                     TDDCVEAALRMHKEGADIIDIGAESTRPGSSPLGQEEEARRLVPAIEAVRRELPHVLI
                     SADTFRSETAEAALDSGADIINDISGGAFDGGMFPLLAKIGNPIVIMHARDVPGGMHH
                     PVTYGDIIGEIITFFSWRMDEAE"
     assembly_gap    4868..4967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4968..5642
                     /locus_tag="PLM30_02910"
     CDS             <4968..5642
                     /locus_tag="PLM30_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54153.1"
                     /translation="AAAQHLKILSVPGHPLSLVLETSEGVITSALLRSPAGIQKILPL
                     EGFVYAGETFAEPYADGDIRKDLLWTITFTRPGDRSRGIYLWIGLTTRIPRAWVIISP
                     LGQTYWDTIPMKVYAPRGTALFVSPNLPAYGDLPQFGGNRTLTFVYTIALTPEGPNFL
                     PVPEVYRQLYTITATIRDAEQITERREAYSRLLEDYETLSRGGKPSTEVIQNFTWKRI
                     LCLDWR"
     gene            complement(5677..6537)
                     /locus_tag="PLM30_02915"
     CDS             complement(5677..6537)
                     /locus_tag="PLM30_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR54154.1"
                     /translation="MDHRIYDLEGIDNQWCPGCGDFQILEALKKALAGLELPPEKVVL
                     FSGIGQAAKTPHFMKGNFFNGLHGRSISNATGMKVANRALTVIAIGGDGDMYGEGGNH
                     FFHTIRRNPDITNIAHNNMVYGLTKGQASPTSAVGFKTPVQVEGVFSEPFNPVAVAVA
                     LGASFVARAYSANIDQTVGIIKEAILHKGYALVDIFQPCVTFNKVNTYAWFKANTFDL
                     PGDHDPSDRMAAMERAQEGPPYPLGVLYKVEGRPTFEDSMGCYHEDDTPLYRRAYGPE
                     RIKDLLALFR"
BASE COUNT         1137 a         1968 c         1814 g         1341 t
ORIGIN      
        1 ccggggggcg cgtccccgat ggtaccgtac cggtcgtagg cggccctctt ctgagggtcc
       61 tgaagcacct cgtaagcgag gttaattttc ttgaacctct cctcggcacc gggatcgccg
      121 gggttggcat cggggtgatg tttccggacc agtttccggt aagccttctt gatctcttcc
      181 tgcgaggcgt cccgggaaac accgaggatc gcgtacaggt cccggctgtc ctcagcgggc
      241 accgaacgtc actccctagg gctttccgtt ctcttcgaat tcggcgtcga ccgtatcgcc
      301 gtcggtaccc ggcccggaag acccctggtg agcctcctgc tgccccgcgg cttgatcgta
      361 aagccgggcg gacatctcgt gaaggctctt cgtcagtatt tcgcaggcct ggcggatctc
      421 cccggggtcc tcaccggcca gtttgccccg gagatggtcg atctcttccc tgacccgggt
      481 tttttcggca tcggtcaccc tgtcgccgag gtcggagagg gtcttctccg tgctgtatat
      541 cagcgaatcc ccctcgttcc gggcatcgac cagttccttc ctcttacggt cgctttcaag
      601 gttctcctcg gcggtgcggc gcattctctc gatctcggcc tcggtgagcc tcgaggactg
      661 gatggtgaca tgttgctccc gggaagtccc cttgtccctg gcgctcacgt tcaggatccc
      721 gttcacgtcg atatcaaagg tgacctcgat ctggggcatc cctcgcggcg cgggcgggat
      781 cccgtcgaga aagaacttgc ccagcgtgac gttgtcggcc gccatggaac gctccccctg
      841 gaggacatgg atctcaacct ggggctggtt gtccgaggcc gtggagaaga cctggctttt
      901 cgaggcgggt atggccgtgt tcctgtccaa tatcttggta aagacgcccc ccagcgtctc
      961 aatgcccaat gagaggggcg tcacgtccac caggattatg tccttgtgct cgcccgtgag
     1021 gatagcggcc tggatcgccg ccccgacggc gacgcactcg tcggggttta tccccttcgt
     1081 gggttccttc cccaggagtt cgactatctt cctctgtacc atgggcatcc tggtcgcccc
     1141 gcccacgagg agcaccttgt ggatctcctc cctggtcagg ccggaatcgt tcatggctct
     1201 ccgggtcggt tccacgacct tctcgagcag tgatcgcgtg atctcctcga agcgcgccct
     1261 ggtcaggctc agctcaaggt gcttggggcc agtgctgtcg gcggtgatga agggcagcga
     1321 aatggaggtc tccgtcatgg aggatagttc caccttggcc ttctcggcgg cctcgcggag
     1381 gcgctgcagg gccatcttgt ccctggaaag gtcgatgcct tcgtttttct tgaactccga
     1441 caccatcagg tcgacgatct tctggtccca gtcgtcccca cccaggtggt tgtcccccga
     1501 ggtcgccaac acctcgatga ccccatcttc gatgcccacg tcgaggaccg atacgtcgaa
     1561 ggtgcccccg ccgaggtcaa agacaaggat cttgttttcg cctcccttgt ccataccata
     1621 agccagggcc gccgcggtgg gttcgtttat tatcctcagc acctcgaggc ccgcgatctt
     1681 cccggcgtcc ttcgtggcct gccgctgggc atcggtgaag taggccggca cggttattac
     1741 agcctgggtg atcttctcac ccaggtattc ctcggcgtcc cgcctgagct tctgaagtat
     1801 catggccgat atctgctgcg gggtctgcga ggccccnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnccca ctcccttgac ataggactcg atgtcggacc ggttgcccga
     1981 aagcgccctg tccaggttgt cgaggacggg gataagagcg atggcggcat cctcaccggc
     2041 catggatctg agcctttcct gctcgcgctc gaccctctgg cgatagttga aaaaatccgc
     2101 ccgggccctc gctatggcat cgcccagggc cttgttctcc tcgctgagcc gctccaccag
     2161 ggccttggcc gaccccagtt cggaaagcaa cccttcattg tcggccgacc cctcctcgtg
     2221 gggttctttt tcgatggtgg cttcaagtcc gccttccccg ctgtcacctc gggatccatc
     2281 ttcagggttt tcctgcggcc ttttcccctc gggtcttatc atcgatgtac ctccttggcg
     2341 ctttcgccgt tctcaccgtt ttcgccctta ccggcgatgg tcccgagaac accctcgaga
     2401 accgaaatgg acctctcgta atccatcctc atcggcccga tgaggccgac cacggccttc
     2461 cggccgccgg ctatcgtgga ggccatcagt atggaacagt cgggcatatt cagttccggg
     2521 ttctcatccc ctatggtcac gcggaggccc ttttcggtcg aacagctgcg gaccagcatc
     2581 ccgatggact cgtcctcctc gaggagcgcc attatggtct gcaagcggcc gaggtcgctg
     2641 aaatcgggca gtcccaggac attatgggtg cccccggtga agaactggaa gggttgctcc
     2701 agcagcaagc cgtccatctc attcaaagcc gaccggcaac tctcggcgta cctctccagg
     2761 gcatcttcca cgtaggcctg gagtgcaccc ctgacctccc cccaggacct cccgacggcc
     2821 acggtggaga cacggcggga tagttcatcg agggcctcct gggtcatgtc gcagggcaag
     2881 gtggatattt tgtggtggat gagccctccc ttgagcacca gcaatatcag caccgtgctg
     2941 tgccccatac ggagaaattc gaccctttcc aggacaggct cccgctcggg tcccagagct
     3001 gctaagccga tatagctggt tatcctgccg aggagcctgg tggcgtagat aagggcttcc
     3061 tccacgtcct ttcttcgttc ccggagttcc tttatccacc cttcgctctc ggaggggaag
     3121 gacctctgcc gtctcaggat tgcgtccaca tagacacggt acgccttagg tgtagggatc
     3181 cggccggctg aggtgtgggg ctggtaaagg tactccgact cctccaggtc ggccatctcg
     3241 ttcctgatgg tcgccgcgct gcggcccttc aggaacttcc tggcgatagt gcgtgaccca
     3301 acaggctcac cggtcttgat atattcgtaa accaccgcga ggaccacttc cagttgtctt
     3361 tcgctaagca ctcccgcagc aactccttgt cttttctatt agcactccca ctcaaagagt
     3421 gctaagggac acccgtccga atggtagatt agcaatggta tcgtctattg tcaacactgg
     3481 tcagaaacat gggcacccca attgcgtccc tgttgaccta gaataggcct tggtgaataa
     3541 acctttcctc ggggggtctc ctttgaccga aaacacgacg acggtgatac ttgtaaggca
     3601 tggtgagtgc gaaggcaaca gggaaggact cttcaggggg aggaccgact tcccnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgctc aaaccgctcc tggcggaggt
     3781 cctggatata ggtgaccctt acttctggaa ggtccacctc gacacggcct ccttttcgat
     3841 cctgagacat gaaaaagaca ggggctactg cttgaccacg ctgaaccaga cctaccacct
     3901 gggccggttc atcagcgaat gggtctgacg caaggagacc ccctatcctc gacgggagag
     3961 ctggaataat gtcgctatac cggatcgaga tcgagagcag gaaggacctg gccgacctgg
     4021 tccgcgtcac cggggccgac gagaggagtt tttacttctt cgagcgaaag agacggatgc
     4081 tggccttttt catccccgag gttgacttca gggcggccaa cgccctaaaa caggaactcc
     4141 tttcccgggg cggtgacgcg atcgtccaca ggggcgccat agcggggtcg gtgcccacgt
     4201 ccagcgtcgt gatcctgggg accctcaaga ccctggaaga tctctcggtc aaattgagat
     4261 cgatgcccta ctgggggctg gacgtggtaa aagaccaact cctgcgggcc ctctcctctc
     4321 tcggcgttaa acgatggaac ctgaaggtgc cgggccggga cgacctcgag ttgggcggtc
     4381 atacaaaggt gatgggcgcg ataaacatca ccaccgattc tttttacccc gggagcaggg
     4441 tcaaggggac cgacgactgt gtcgaggcag cccttcgaat gcacaaggag ggcgctgata
     4501 tcatcgacat aggggcggag tcgactcggc cgggttcaag tccgctcggc caagaggagg
     4561 aagcccgccg cctggtcccg gccattgagg cggtcaggag ggagttgccg catgtcttga
     4621 tctccgccga caccttccgt tcggagaccg ccgaggccgc ccttgattcc ggggccgata
     4681 tcatcaacga tatctcgggg ggggccttcg acggggggat gtttcccctg ctggcgaaaa
     4741 taggcaaccc catcgtcatc atgcacgcca gggatgtccc cggggggatg caccatcccg
     4801 tgacctacgg ggatataatc ggcgagatca taacattctt ttcctggaga atggatgagg
     4861 cggaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gccgcgcagc
     4981 acctgaaaat actctccgtt ccggggcatc ccctgagcct ggtcctcgag accagcgaag
     5041 gggtgataac ctcggcgctg cttcgttccc ccgcggggat acagaagatc ctccccctcg
     5101 agggtttcgt ctatgccggg gagaccttcg ctgagcccta cgccgatggt gatatcagga
     5161 aggacctcct ttggaccatc actttcacga ggcccggtga ccgctcaaga ggaatatacc
     5221 tctggatagg gctcacaact cgtatcccca gggcctgggt cataatcagc cccctgggcc
     5281 agacctactg ggacacgatc ccgatgaagg tctacgcccc caggggcacc gcgctcttcg
     5341 tctcgcccaa tctgcccgcc tacggcgacc tgccccagtt cggcggcaac aggacgctta
     5401 ccttcgtgta caccatcgcc ctcacccccg agggtccgaa cttccttccc gtgcccgagg
     5461 tctatcgcca gctttatacc atcacggcca ccatcaggga tgccgaacag atcaccgaac
     5521 gccgggaagc ctactccagg cttctcgaag actacgaaac cctttcaagg ggagggaaac
     5581 cttccacgga agtgatccag aacttcacct ggaagcgcat cctctgcctg gactggagat
     5641 gagaccaagg ggcggggagc atccccgccc ccggtgctac cggaataagg ccaggaggtc
     5701 cttgatcctt tccgggccgt aggccctccg ataaaggggg gtgtcgtctt cgtggtaaca
     5761 gcccatggaa tcctcgaagg tgggccttcc ctcgaccttg taaaggacac ccagcgggta
     5821 gggcggccct tcctgtgccc gttccatggc ggccatcctg tccgaagggt catggtctcc
     5881 cgggaggtca aaggtgttcg ccttgaacca tgcgtaggtg ttgaccttgt tgaaggtcac
     5941 gcagggctgg aagatgtcca ccagggcgta acccttgtga agtatggcct ctttaatgat
     6001 ccccaccgtc tggtcgatgt tggccgagta ggccctcgcc acgaaggagg cccccagggc
     6061 gacggccacg gccacagggt tgaagggctc ggaaaagaca ccctccacct ggaccggtgt
     6121 cttgaaaccc acggcgctgg tcggtgaggc ctgccccttg gtaagaccgt agaccatgtt
     6181 gttatgggct atgttcgtga tatcggggtt cctgcggatg gtgtggaaga agtgattccc
     6241 accctcgcca tacatatctc cgtcaccgcc gatggcgatc acggtcagcg ccctgttcgc
     6301 gaccttcatg cccgtggcgt tcgatatcga tcggccgtgc agcccgttga aaaaattgcc
     6361 cttcatgaaa tgaggcgtct tggctgcctg gcctatcccc gaaaagagga ccaccttttc
     6421 aggggggagt tcaagcccgg caagggcttt tttcagggct tccaggatct ggaaatcccc
     6481 gcacccggga caccactggt tgtctatccc ttccaggtcg tatatccggt gatccatctg
     6541 aaacaccccc tatcggagca
//
LOCUS       DAOSRG010000046         6321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11354_length_6321_cov_8.664220, whole genome shotgun sequence.
ACCESSION   DAOSRG010000046 DAOSRG010000000
VERSION     DAOSRG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11354_length_6321_cov_8.664220"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="PLM30_02920"
     CDS             complement(<1..1897)
                     /locus_tag="PLM30_02920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOR54155.1"
                     /translation="MIKLTVIVTTVLLAVLLLSRPSFAGGGSLIPMEDFFRNPEKSGF
                     RISPDGTRISWRAPWERRLNIFVQKIGEEEAVRVTSATERDILGYFWSGNDRILYLQD
                     SGGDENYHLFAVDAGGSGAKDLTPFEGVRVTIVDPLEEIDDEVLIGMNRRDPRVFDVF
                     RLNIDTGELTLVQENPGDIEGWVTDHEGKLRAAVRTDGVNQSLLYRDREEDEFRTLLT
                     TDFRETVDPLFFTFDDKKLYVSSNLGRDKSAIYVFDPAKAASEELVFEHPEVDVYGLM
                     QSKKRKVITGVAYVTDRARYHFFDRERADLQEELESRLPGREVTVTSMSKDEEKVLVH
                     AGGDQTQGAYYFYDRTTGDFRELAQLSPWLNEDELAPMKPVQYTARDGLVIHGYLTLP
                     AHSDGKNLPVVINPHGGPWARDSWGYDSEVQFLASRGLAVLQMNFRGSTGYGRAFWEA
                     GFKQWGRAMQDDITDGVQWLIDQGIADPKRVGIYGGSYGGYAVLAGLAFTPDLYACGV
                     DYVGVSNIFTLLETIPPYWELGRKMMYEQIGDPEKEKDLLEAASPVFHADRIKAPLFV
                     AQGANDPRVKKAESDQIVEALRKRGVDVEYMVKDNEGHGFLNEENRFDFYRAMERFFS
                     KHLGSLSE"
     gene            complement(1902..2315)
                     /locus_tag="PLM30_02925"
     CDS             complement(1902..2315)
                     /locus_tag="PLM30_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR54156.1"
                     /translation="MKTYTEYLWFETRKRKELVRITSDLEAILGRSGVTDGMMLLSAM
                     HITSGIIVNDDEPGLHRDILEWLEKTAPEDPSYAHHRTGEDNADAHLKRILVHHQAIL
                     PVTGGKLDLGPWEQCFYAEFDGRRRKRVVVKVMGV"
     gene            complement(2478..3335)
                     /locus_tag="PLM30_02930"
     CDS             complement(2478..3335)
                     /locus_tag="PLM30_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR54157.1"
                     /translation="MHIDAHVHVYPPEIEKNWKKIAETEPHFGTLCAGKAHKWGTVED
                     VVARMEIDGLDEAWVTGVAFRDQGLCRLCNDYVMEALKKYPGKIRGLAVVNPLTRDFE
                     SEIVRCREAGFIGVGELLPYGQVFDISDSRETWRLAAACQENGLFLLLHVAEPVGRDY
                     PGKGGMGPREAAAFCENHPEVQVLFAHMGGGLWAYEAMRCMKLVLENAWYDTAAAPFL
                     FGPEVFRAAVAAGAGGKIIYGSDFPLLCADRYEKMFLASGLDEEALSRIRGGNALALL
                     EKVRSFPGL"
     gene            complement(3345..5867)
                     /locus_tag="PLM30_02935"
     CDS             complement(3345..5867)
                     /locus_tag="PLM30_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54158.1"
                     /translation="MRKKIILFDRIGTLLGRSTLLIALVVATFFLLVSPAFAKIVGIT
                     VTIYPEEVRSSARFIIQGQEYSSGQTASVEGKTNYPISFTQVPGWKAPKDDVITVKNS
                     DIQWPGNDRSKSTYEQLEEVPGIEIHDISKWPLWPKTSSNANVWEPYSFTSIGRDVVS
                     WVTFEPLLNNPYFWLSDLRLVIRTGELQYASVRPRFDRISQQYKYIAWDDLRSFPSSL
                     QDMQDDLREYAVIPSFMSNWMIFTENDVPQVFPNQDFDDSWFSFNGWMAFLRGDAGFK
                     LEDSGHAPPLTMWAEDGWLTLFVDRNSVPSAWIDENGKGKVFVFLPTNQGVLNCFEIS
                     DDPDNAGADRVWSLTPFPAFQQAVFNQVYSHQGNGQYARLTLLDGPVYVHDLQDKNGK
                     WKRILVGSTGLGTEQVNKLGAAWIAEGAPAPGASPVITGDSQVFGIYAIDVTDPLDPQ
                     PLWSVTNINCIRSGGATVKRLDIVSPRVDTGSADFTAYEDVDFMTTKPLIGFTETLEG
                     SRIWHLLLVTIDKEGRYLWINADPLTGEVLTSGNFVGFQGEPLESLPGEDTRKMPSGF
                     NHEDWYPSRILAAYPGEGGLPALSDVYIYLSNGTFYSWDLNGYDWRYVGSTAPKPSKI
                     FNVYTNPSHQIPAPPITDFDIVYIGGDTYLAATAPLVYPGYGQSHDTYGLLIINLSRI
                     QRDGITELNTQGAQGQSGDTKLRLLETGVAMVQLQTGKGAHAYDFEQLAASPLFVNYR
                     LYQALYSTDAALSRLYTLDMSFFAGLDKRKGNVNLPDDAYIDDPDNAFTAMFIDSEGF
                     LVILDEEGNVVNDPEQVLELGGTGTETGLKGKGITTIYWKTL"
     gene            complement(5864..>6321)
                     /locus_tag="PLM30_02940"
     CDS             complement(5864..>6321)
                     /locus_tag="PLM30_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54159.1"
                     /translation="LYNAAQSGIEKAKMVLWENRNDLKTDPLMYSGNITAIYAQKSNS
                     TPLGPIQFTIDPGISVEVLIFDCNYELSAGSFSDLSDEERKLLPPRFPGGTGERTVVS
                     SVPPLGTSAIMDPGRFYGGGAGERRFVIRSSASKDGKDFEIESMVVVRK"
BASE COUNT         1286 a         1812 c         1813 g         1410 t
ORIGIN      
        1 gctccgacag gctgccgagg tgcttggaga agaagcgctc catcgcgcgg tagaagtcga
       61 agcggttctc ctcgttgagg aagccgtggc cttcgttgtc cttgaccatg tattccacgt
      121 ccactcctct tttccgcagg gcctccacga tctggtccga ttcggccttt tttaccctgg
      181 ggtcgttggc cccctgggct acgaagaggg gcgccttgat cctgtcggcg tgaaagaccg
      241 gcgaggccgc ctcgaggagg tccttttcct tttcggggtc gccgatctgc tcgtacatca
      301 ttttgcggcc cagttcccag taggggggga tcgtctcgag gagggtgaag atgttggaga
      361 cccccacgta gtccacgccg caggcgtaga ggtcgggtgt gaaggcaagg cccgccagga
      421 cggcgtaccc gccgtaggag ccgccgtaga tgcccaccct cttcgggtcg gctatgccct
      481 ggtcaatgag ccactggacc ccgtcggtga tgtcgtcctg catcgccctc ccccactgtt
      541 tgaagccggc ctcccagaag gcgcgcccgt agcctgtgga gccacggaag ttcatctgga
      601 gcaccgccag tccccggctg gccaggaact ggacctcgct gtcgtacccc caggagtccc
      661 gcgcccaggg gccgccgtgg gggttgatca ccacggggag gttcttcccg tcgctgtggg
      721 ccggcagggt caggtagccg tggatgacca ggccgtcccg ggcggtgtac tgcacgggct
      781 tcatgggggc cagctcgtcc tcgttgagcc aggggctcaa ctgggccagt tcgcggaagt
      841 cccccgtcgt ccggtcgtag aagtagtagg cgccctgggt ctggtcgccc ccggcgtgca
      901 cgagtacctt ctcctcgtcc ttgctcatgg aggtcaccgt gacctccctt ccggggaggc
      961 gggactccag ttcttcctgg aggtcggccc gctcacggtc gaagaaatgg tacctcgccc
     1021 ggtcggtgac gtaggccacg ccggtgatga ccttccgctt tttcgactgc atgaggccgt
     1081 agacgtccac ctcggggtgc tcgaagacca gttcctcgct ggccgccttg gcggggtcga
     1141 agacgtagat ggccgacttg tccctgccta ggttggagga gacgtagagc tttttgtcgt
     1201 cgaaggtgaa gaagaggggg tcgacggtct ccctgaagtc ggtggtgagg agggtcctga
     1261 actcgtcctc ctcccggtct ctgtagagga gggactggtt cacgccgtcg gtcctgacgg
     1321 cggcccgcag tttgccctcg tggtcggtca cccagccttc gatgtccccc gggttctcct
     1381 ggacgagggt cagttcaccg gtgtcgatgt tcagacggaa gacgtcgaag accctcgggt
     1441 cccggcggtt catcccgatg aggacctcgt cgtcgatctc ttccaggggg tccacgatgg
     1501 tgacccggac gccctcgaag ggggtgaggt ccttcgcccc ggagccgccg gcgtcgacgg
     1561 cgaagaggtg gtagttctcg tccccgccgg agtcctgcag gtagaggatg cggtcgttgc
     1621 ccgaccagaa gtagcccagg atgtccctct ccgtcgcgga ggtgacccgc acggcctcct
     1681 cttcgccgat cttctggacg aagatgttga gccgccgctc ccagggcgcc cgccaggaga
     1741 tccgcgtccc gtcgggggag atccggaagc cgcttttttc cgggttcctg aagaagtcct
     1801 ccatcgggat gaggctcccc ccgccggcga aggacgggcg ggaaagaaga agaacagcca
     1861 gcaggaccgt cgtcacgata accgtcagtt tgatcattcg ttcatacccc catgaccttg
     1921 acgaccacgc gcttcctgcg gcggccgtcg aattccgcgt aaaagcactg ctcccagggc
     1981 ccaaggtcga gcttgccgcc cgtcacgggc agtatcgcct ggtggtgtac caggatgcgc
     2041 ttgaggtggg cgtcggcgtt gtcctcgccc gtgcggtggt gggcgtagga cgggtcctcg
     2101 ggggcggtct tctccagcca ctcgaggatg tcccggtgga gccccggctc gtcgtcgttc
     2161 acgatgatgc ccgacgtgat gtgcatggcc gaaaggagca tcattccgtc ggtcacgccg
     2221 ctccttccca ggatggcttc aaggtccgac gtaatcctta ccagttcttt ccttttcctc
     2281 gtctcaaacc acaagtactc ggtgtaggtt ttcattgaac atcccctttc gttgagcctg
     2341 gaaacggaaa tagggtagca gaaacaggag ggaagaaaaa gactcgagaa ccctttcccc
     2401 ggaataggat tatggcaaac ctttcccaat tcgtggcagg gccggcgcgt tgtacaaaaa
     2461 aaggaagggt gactttatta aaggccaggg aaggagcgga ccttttcgag cagggccagg
     2521 gcgttgcccc ccctgatcct tgacagggcc tcctcgtcga ggccggaagc caggaacatc
     2581 ttctcgtacc ggtcggcgca gagcaggggg aagtcgctcc cgtagatgat cttgccgccc
     2641 gccccggccg cgacggcggc gcggaagacc tccgggccga agaggaaggg cgcggcggcg
     2701 gtgtcgtacc aggcgttctc caggaccagc ttcatgcacc gcatggcctc gtaggcccag
     2761 aggccccctc ccatgtgggc gaagaggacc tgcacctcgg ggtggttctc gcagaaggcg
     2821 gcggcctccc gggggcccat gccgcccttg ccggggtaat ccctccccac gggctcggcc
     2881 acgtggagca gcaggaagag gccgttctcc tggcaggccg cggccagccg ccaggtctcg
     2941 cggctgtcgg agatgtcaaa gacctgcccg tagggcagga gttcgcccac gcctatgaag
     3001 cccgcctcgc ggcagcggac tatctcgctt tcgaagtccc gggtgagggg gttcaccacg
     3061 gccaggcccc ggatctttcc cgggtacttc ttcaaggcct ccatgacgta gtcgttgcag
     3121 agacggcaaa ggccctggtc gcggaaggca actcccgtga cccaggcctc gtccagcccg
     3181 tcgatctcca tccgggcgac gacgtcctcg acggtccccc acttgtgggc cttgccggcg
     3241 cagagggtgc cgaagtgggg ctccgtctcg gcgatcttct tccagttctt ctctatctcg
     3301 ggcggatata cgtgtacgtg ggcgtcgata tgcatggcgt tattttacag ggttttccaa
     3361 tatatggtcg taataccctt tcccttcaaa ccagtctctg ttccagtacc gccgagttca
     3421 agaacttgct ccgggtcgtt aaccacattg ccttcctcgt ccaggatgac cagaaatcct
     3481 tcagaatcaa tgaacatcgc ggtaaaggca ttatccgggt cgtcaatata tgcgtcgtca
     3541 ggcaggttaa cattaccctt tcttttatct aagcctgcaa aaaaactcat atcaagagtg
     3601 taaagacggc tcaatgcagc atcggtactg taaagagcct ggtagagacg atagtttacg
     3661 aaaaggggag aagctgccag ttgttcaaag tcataggcat gagcgccttt ccccgtctgc
     3721 agctgtacca tggcgacccc tgtttccaat agacggagtt tcgtatcccc tgattgtcct
     3781 tgcgccccct gggtgttcag ttcggttatt ccatcccttt ggatcctgga taggttaatg
     3841 atcagaaggc cataggtatc gtgtgactga ccataaccag ggtaaacaag cggggccgtg
     3901 gcagctaggt aggtgtcccc tcctatgtag acaatgtcaa agtccgttat tggaggggct
     3961 gggatctgat gactgggatt ggtatataca ttgaagatct ttgaaggttt aggagcggtg
     4021 gaacccacgt atctccagtc gtacccgttc aggtcccatg aatagaaggt tccgtttgac
     4081 aggtaaatat atacgtcgct cagggcgggc aaaccgcctt cgcctggata ggccgccagt
     4141 atcctggagg gataccagtc ttcgtggttg aagccagacg gcattttccg cgtatcttca
     4201 ccgggcaaac tttccaaagg ctcaccttga aaaccgacaa aatttccgct ggttaaaacc
     4261 tctcccgtca acgggtctgc atttatccac aagtatctac cttccttgtc gatcgtaaca
     4321 agaagcaaat gccagatcct ggacccttca agggtttcag taaagccgat caaaggtttg
     4381 gtagtcatga agtcgacgtc ttcgtaagcg gtgaaatcgg ctgaccctgt atccacacgg
     4441 ggagaaacaa tgtcgagtct tttgacggtt gcgccgccgg aacggatgca gtttatgttt
     4501 gttaccgacc aaaggggttg agggtcaagg ggatcagtaa catcaatagc gtagattcca
     4561 aacacctggc tatcccctgt gataacgggg gatgctccgg gtgccggggc tccttccgct
     4621 atccaggcgg ctcccagttt gttgacctgt tccgtcccca accccgttga acccaccagt
     4681 attcgcttcc attttccatt tttatcctgg aggtcatgga catagaccgg gccatcgaga
     4741 agagtcagcc ttgcgtactg cccattgcct tgatgggaat agacctggtt gaagacagcc
     4801 tgctggaagg cgggaaaagg cgtcagcgac cagacccgat cagccccagc attatccggg
     4861 tcgtcggata tttcgaagca gttcaaaacc ccctggtttg tcggtaaaaa aacgaagacc
     4921 ttacctttgc cgttttcatc gatccaggca gaaggcaccg aatttctgtc cacgaaaagg
     4981 gttagccagc cgtcctcagc ccacatcgtc aagggaggcg catggcctga gtcctcaagt
     5041 ttaaagcccg catcacccct gagaaaagcc atccaaccgt tgaaggagaa ccacgaatcg
     5101 tcgaaatcct ggttggggaa gacctgagga acgtcatttt cggtgaagat catccagttt
     5161 gacataaaag aaggaattac cgcgtattcc ctgaggtcat cttgcatatc ctgaagtgag
     5221 gaagggaaac ttctcaagtc atcccaagcg atatatttgt attgttgtga gatcctgtcg
     5281 aaacgcggcc taacgctcgc atactgtagt tcacctgtcc ttatgactaa cctcaggtca
     5341 gaaagccaga agtaaggatt attcagcaac ggttcgaaag taacccaaga gacgacatca
     5401 cggccaatgc tcgtaaagga gtaaggttcc cagacattcg cgttcgagga tgttttaggc
     5461 cacaatggcc acttcgatat atcatggatc tctatccccg gaacttcctc caattgttca
     5521 taggttgatt tggatcgatc attccctggc cattggatgt cactgttttt aacggtaatc
     5581 acatcatctt tcggtgcctt ccatcccggg acctgggtaa aggaaatggg gtaattagtc
     5641 ttgccctcca ctgaagccgt ctgacccgaa ctgtattcct gtccttgaat tatgaatctt
     5701 gctgagcttc tgacttcttc aggataaatt gtgactgtta ttccaacaat ttttgcaaag
     5761 gctggagaaa ccagcaagaa aaacgtagct actacaaggg cgatcaatag ggtcgaccga
     5821 ccgaggaggg taccaattct atcaaaaaga attattttct ttctcatttt ctcaccacca
     5881 ccattgactc aatctcaaaa tctttgccat cctttgaggc actggaacga atgacaaacc
     5941 ttctctcccc cgcccccccg ccatagaacc tgccagggtc cattatggcg gatgttccta
     6001 ggggtggaac cgatgacacc actgttctct cgccggtccc gccaggaaag cgcggcggga
     6061 ggagtttcct ctcctcatcg gaaagatcgc tgaaagaacc agcggagagc tcataattac
     6121 agtcaaagat aaggacttcg acggaaatac caggatcaat cgtgaactgt atcggcccca
     6181 agggtgtgct gttgcttttc tgtgcgtaaa tcgctgtaat gttcccactg tacatcaacg
     6241 ggtcggtctt gaggtcgttc ctgttttccc aaaggaccat cttggccttt tctatcccgg
     6301 actgggccgc gttgtagagt t
//
LOCUS       DAOSRG010000047         6288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11443_length_6288_cov_10.062410, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000047 DAOSRG010000000
VERSION     DAOSRG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11443_length_6288_cov_10.062410"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLM30_02945"
     CDS             complement(<1..602)
                     /locus_tag="PLM30_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR54160.1"
                     /translation="MLKSCNPLLGPLVPVVKLLGAMLGKDYEIVLHDVSGEEPFIVAI
                     ENGEISGRDINSSMTDFGYFLMSSPEAEGVDHVANYPSETGTGKSLRSGVALIRDGDH
                     KLIGFLCINCDMARGQVLKDLGGFLTELQPLSFRGLEFERFSRVNDTSAEEILEKVRH
                     DLGKPLNYLNRSERAHCIEMLEGYGFFNLKGAVRLLARKMG"
     gene            817..1806
                     /locus_tag="PLM30_02950"
     CDS             817..1806
                     /locus_tag="PLM30_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54161.1"
                     /translation="MRLITKEEMRRVFSMNEAIEAMKQAFRLFSEGRSVVPLRTNIDV
                     GKYEGQALFMPAYVEETQSLGLKIVSVFPGNAKLGKPVVPATMVHMDSRTGEVLAIMD
                     GTYLTQVRTGAAAGAATDLLAREDATVGALIGAGGQSSAQLEAMLTARKLKEVRIFDA
                     DETRAKETAKTQGEIWEKAGVRIVTSKSGEEAVDGADVITTVTTARNPVFPAEAVKRG
                     AHVNGIGSYRPDMQEMPPELFLKAQKIFVESREAVLAESGDLINPIGKGLFTEKIITG
                     ELGELIAGKVVGRESDGDITVFKTVGIGVQDVVAAGEIFRKAVEKGVGTEFDL"
     gene            1956..3182
                     /locus_tag="PLM30_02955"
     CDS             1956..3182
                     /locus_tag="PLM30_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54162.1"
                     /translation="MKKKVLGSLILAVFLAALISTPILSAEKKEITIGAFMPLTGDFA
                     SYGARGRVAMEKAEADIAKFTSGANLPITIKFLYEDTETKANVTLEKIKAMAAQGVKV
                     AVGLLDSSDIRLAMGYANANKIALISSFSTVAELGVADDYVFRPIPHDDMEGVALAKL
                     VETMGFTHVALLVRKDPCDLSIASSFAKEFEAVGGTILERVDFAGGTKEFSGELSSLE
                     RAVGPAVEKHGADKVAVISLTWEDLAIILSQAQARKSPLLSLTWTGGDAVAQSTVILR
                     DAGDAAAKVSVIAPMYSAPKSIKREELLAYAQEKIGETLDIYSLVAYDCAWLAALSAI
                     CAQSGDGTAIKDALPYVASNYYGASGWAELDELGDRKALNVEFFAVKEVEGKHAWVKV
                     SEYDAATGSLAEVSSK"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3309..3878
                     /locus_tag="PLM30_02960"
     CDS             <3309..3878
                     /locus_tag="PLM30_02960"
                     /inference="COORDINATES: protein motif:HMM:NF023816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54163.1"
                     /translation="EDKEITHWPMHRICKEGMVRSFQLPAPFGGLSVLGNLLVACESA
                     CGESLLKCPLKALWKTTEKDNVREAFAMAEAMELTPYWDAVMSETSTGHIKLAEIGRA
                     LLAGAKLVILDEPICGVDPVMADEVFSFLIRLRDEQGLTFLVIEHRLDIALKYADYVY
                     VMNQGKLIAQGSVEEVLNDSQVKEVYLGS"
     gene            3875..4591
                     /locus_tag="PLM30_02965"
     CDS             3875..4591
                     /locus_tag="PLM30_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54164.1"
                     /translation="MNNLLKIENLNAGYGGIHVLFDVNLDCTRGEAVAIVGPNGSGKT
                     TLLNSIFRLADIFSGSVILDGTEMQKEKSHKVTGRGVGYLRQEGNVFPGLTVEENLKM
                     GGFNLPKGEVGERMEEVLSLVKPVVPFLRRTAGTLSGGEREMVALAVSLMCRPKLLLL
                     DEPTSGLSPIASAQIAELIKNLKDKTRLSMLIIEQNLKILFDVVDKCYLLVSGRIIFS
                     GGPGELQCDKELMRTYLGLA"
     gene            4607..5506
                     /locus_tag="PLM30_02970"
     CDS             4607..5506
                     /locus_tag="PLM30_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54165.1"
                     /translation="MASFIDALTYSALLGLMGFGLTLIRRTTGVWNVAHGEVVTIGSY
                     VALTATSFLAHSPYFYLPLAFLVCAGVSGIIFLAGVEPIRAKGASSILLLVVTIAISL
                     IMIAVINIYADYLQNVFHLSSKNFLFARFDFTLLGQMGILWAAWAALAALTISYYLVL
                     QHTRLGLALRALAEQPTLAVVMGANVKSLSLLSWCLAGGAAGLAGCLFPMRFQCHPGV
                     GTSMIATMFAVSIFGGIEKMYGPLLAGLVLGFAQIYGIDLLSSWVGPGVIPFKDLIPM
                     IVLLLTLQFLPEGLSGFVGRRRG"
     gene            5510..>6288
                     /locus_tag="PLM30_02975"
     CDS             5510..>6288
                     /locus_tag="PLM30_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54166.1"
                     /translation="MSGQWFLFSVDLLALFGIYLALNLSLRLQLGYTGIANFGLVLAF
                     TGGAYVGGWLPIRLGMWLVEVDPSLADDMIWNNVIIASQVDLSLHGSPGTAMTILLIT
                     LLAAGAAGALLGLLSGYPAVRLGGDYLAISLLAFGEVLAIIGNNYDPVVGGPLGVRIP
                     DVWAWAGDWRFMAASLANCALALGVWLYLDSIEKSPMGRMLRAIRDNPDAASSLGKNV
                     ARVRLNVMVTGGIICGLAGAMYTLYTTGVAPSALSRFDWTFL"
BASE COUNT         1298 a         1757 c         1848 g         1285 t
ORIGIN      
        1 cccattttgc gcgccagcaa ccggaccgcg cccttcaggt tgaaaaaacc gtacccctcg
       61 agcatctcaa tgcaatgggc cctttcagaa cggttcaggt aattcagcgg cttgccaaga
      121 tcgtggcgta ccttttcgag gatctcttcg gcggacgtgt cgtttacccg tgaaaaacgt
      181 tcaaattcaa ggccacggaa ggacaggggc tgaagttcgg tcaggaaccc gcccaggtcc
      241 ttgaggacct gaccccgcgc catgtcgcag ttgatgcaga ggaaaccgat cagtttgtgg
      301 tcgccgtccc ggatcagggc cactcccgaa cgcaaggatt tgcccgtccc tgtttcggag
      361 gggtaatttg ccacgtgatc cacaccctcc gcctcagggc tggacatcag gaagtagccg
      421 aaatcagtca tgctggagtt tatatccctt ccggagatct caccgttctc aatggcaacg
      481 atgaagggct cctcaccgga gacgtcatgg agcacgatct cgtagtcctt ccccagcatg
      541 gcccccagga gcttgaccac tgggacgagt ggcccaagaa gggggttgca actctttagc
      601 atttcgtacc tcctttgagt cagatgaaaa gtcatggccc ttcagccaca ggaaagaaca
      661 gggttactag aacccattag tgcaatgggt tctattttcc accttattca acaggattat
      721 ataacataca aaaatatgtt gactggccct ttcttgattg atagactgaa actggcaatt
      781 gtagaataga gcaaagcatt gggggagggg tatcggttgc gattgatcac caaggaggaa
      841 atgcggcggg tcttttcaat gaatgaagcc atagaggcca tgaaacaggc tttcaggctc
      901 ttttccgagg gaagatccgt cgtcccgctg aggacgaata tcgacgtcgg caagtacgaa
      961 ggccaggctc tttttatgcc ggcctatgta gaggaaaccc agtccctggg gctgaagatc
     1021 gtttcggtct tccccggcaa tgccaagctt gggaagcccg tcgtccccgc gaccatggtc
     1081 cacatggact cccggaccgg ggaagtgctg gccatcatgg acggcaccta cctcacccag
     1141 gtccgcaccg gcgcagcggc gggcgcggcc acggacctcc tggccaggga agatgccacg
     1201 gtgggggcgc tcatcggagc cggtgggcag tcctccgccc agctggaggc gatgctcacc
     1261 gctaggaagc taaaggaagt caggatcttt gatgccgacg agaccagggc aaaagagacc
     1321 gccaaaaccc agggcgagat ctgggagaaa gccggcgtca ggatcgtaac gtcgaagtcc
     1381 ggtgaagagg ctgtggacgg ggccgatgtc atcaccaccg tcaccaccgc caggaaccct
     1441 gttttccccg cggaagccgt aaaaagaggg gcccacgtca acggcatcgg gtcttaccgg
     1501 ccggatatgc aggaaatgcc cccggagttg ttcctgaagg cccaaaaaat cttcgtagaa
     1561 tcacgcgaag ccgtcctggc cgagtcgggt gatcttatca accccatcgg caagggcctt
     1621 ttcaccgaaa agattatcac cggcgaactg ggcgaactca tagccggcaa ggtcgtcggg
     1681 cgagagagcg acggagatat aaccgtcttc aagacggtag gcataggtgt gcaggatgtc
     1741 gtcgcggcgg gggaaatatt ccggaaagcc gttgaaaagg gagtgggtac ggagttcgat
     1801 ctctgacgat tgagaggagt gataggcgag aaggagagtc acgatcccct cggcacttct
     1861 gcgagaaagg cgctttcagg gtgatccgca gggccttggg caaaagagaa ccgataggag
     1921 atgaccctgt ttcggggacg aaggagtgat aattgatgaa aaagaaggtt ttgggaagcc
     1981 ttatcctggc tgtgttcctg gccgccttga tctccactcc catcctctcg gccgagaaga
     2041 aagagatcac gataggagcc ttcatgcccc tgaccggaga tttcgccagc tacggggcac
     2101 gcggcagggt tgccatggaa aaggctgaag cggacatagc gaaattcacc tcgggggcca
     2161 acctcccgat aaccatcaaa ttcctgtacg aggacacgga aacgaaagcc aacgtgacgt
     2221 tggagaagat caaggcgatg gccgcgcagg gggtcaaggt cgcggtcggc cttctcgaca
     2281 gttccgatat acgcctggcc atgggctacg ccaacgccaa caagatcgct cttatttcct
     2341 ctttttcgac cgtagctgaa ctgggagtag ccgacgatta tgttttcagg ccgatacccc
     2401 atgacgacat ggaaggtgtc gccctggcca aactggtgga gacgatgggt ttcacccacg
     2461 tagctcttct cgtgaggaag gacccttgtg acctctctat cgccagcagt ttcgccaagg
     2521 agttcgaagc cgtcgggggg acgatcctcg agcgggtgga tttcgccggt gggaccaagg
     2581 agttctctgg tgaactttct tcgctggaaa gagccgtcgg ccccgctgtc gagaaacacg
     2641 gcgccgacaa ggtggcggtc atcagcctga cctgggagga cctggccatt atcctgagcc
     2701 aggcccaggc acggaagagc ccccttctct cgctcacatg gacgggcggt gatgctgtag
     2761 cgcagagcac cgtcatcctc cgagacgccg gtgatgcggc cgcgaaagta tccgtcatcg
     2821 ctcccatgta ttccgcccct aagtcgatca agcgcgagga gctgctcgct tacgcccagg
     2881 aaaagatagg cgaaacgctg gatatctaca gcctcgtagc ctatgattgt gcctggctgg
     2941 cagccctttc cgccatatgc gcccagtcgg gtgacgggac ggccatcaag gatgcccttc
     3001 cttacgtggc ctccaattac tacggggcca gcgggtgggc ggagttggat gaactgggtg
     3061 acaggaaggc cctgaacgta gagttttttg ccgtgaagga agtggaaggg aaacacgcct
     3121 gggtcaaggt ctctgagtac gatgctgcta ccgggagcct cgccgaggtc tcttcaaagt
     3181 aaccggatcc ccgcggccct gccccgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnga agacaaggag atcacccatt ggcccatgca caggatctgc aaggaaggga
     3361 tggtgcgctc ttttcagctc cccgctccct tcgggggctt gagcgtgctg ggcaacctcc
     3421 ttgtggcctg tgagagcgcc tgtggcgaat cccttttgaa atgtccccta aaggcactct
     3481 ggaaaacaac ggagaaggac aacgtccggg aggcctttgc catggccgag gccatggaat
     3541 tgacccccta ctgggacgcc gtcatgtcgg aaacctccac cgggcacatc aaactcgccg
     3601 agatcggacg ggcactcctg gccggcgcga aactcgtcat cctggatgag cccatttgcg
     3661 gcgtcgaccc cgtgatggcg gacgaggttt tctccttcct gatccggctc agggatgagc
     3721 agggccttac cttcctggtt atcgaacaca ggctcgatat tgccttgaaa tacgccgact
     3781 atgtctacgt gatgaaccag ggaaaactca tcgcccaggg ttcggttgaa gaggtcctga
     3841 acgacagcca ggtgaaagag gtgtacctgg ggtcatgaac aacttgttga agatcgagaa
     3901 ccttaacgcc ggttatgggg gtatccatgt ccttttcgat gtaaacctcg attgcacccg
     3961 gggggaagca gtggccatcg taggccccaa tgggagcggc aagacgactc tactcaacag
     4021 tattttccgt cttgccgata ttttttcggg aagtgtgatc cttgacggta cagaaatgca
     4081 aaaggagaaa tcccacaagg tgaccggccg tggagtcggt tacctgaggc aggaaggcaa
     4141 cgtcttccca gggctcacgg tggaagagaa tctcaagatg gggggcttca acctccccaa
     4201 gggcgaagtc ggagaacgaa tggaagaagt gctctccctg gtcaagcccg tcgtgccctt
     4261 cctccgcaga acggcgggga ccctcagcgg cggggaaagg gaaatggtcg cccttgccgt
     4321 atcgctcatg tgcaggccca agctgctatt gctcgacgag ccgaccagcg gcctgtctcc
     4381 catagcctcg gctcagatag ccgaactgat caagaacctg aaggacaaga cacgcttgag
     4441 catgttgatc atagagcaga acctgaagat cctttttgac gtcgtcgaca aatgctacct
     4501 gctggtcagc gggcgaataa tattttccgg cggacccgga gagctccaat gcgacaagga
     4561 gctcatgaga acctatctcg ggttggcctg acaaggggcg atgaccttgg cttcatttat
     4621 tgatgctctg acctactccg ctctgctcgg cctgatgggt ttcggcctca ccctgatacg
     4681 gcggacgacg ggagtctgga acgtcgccca tggagaggtc gtcaccatag gttcctacgt
     4741 tgccttgacg gccacgtcct tcctggccca tagcccttat ttttacctcc ccctggcgtt
     4801 cctcgtctgc gccggggtga gcgggatcat attcctggcg ggtgtcgagc ccatacgggc
     4861 gaagggagct tcctcgatac tcctgctggt cgtcaccatc gcgattagcc tgataatgat
     4921 agcggtcatc aacatttatg ccgattacct ccagaacgtt tttcaccttt cctccaagaa
     4981 cttccttttc gccaggttcg atttcaccct tttggggcag atggggatcc tctgggcagc
     5041 gtgggccgcc ctggccgccc tgaccatcag ttattatctg gtgcttcaac atacacgcct
     5101 ggggctggcc ctgagggcgc tggcggagca accgaccctg gccgttgtga tgggcgccaa
     5161 cgttaagtcc ctttcccttc tctcatggtg cctggccggg ggcgccgcgg gccttgccgg
     5221 ctgtctcttc ccgatgagat tccaatgtca ccccggcgtg gggacgagca tgatagcgac
     5281 catgttcgcc gtcagtattt tcggcggcat cgagaaaatg tacggtccgc tcctggcggg
     5341 actggtcctt ggttttgccc agatatacgg gattgacctt ttatcctcgt gggtcggacc
     5401 cggggtgata cccttcaagg accttatacc gatgatcgtg ctcctgctca ccctccagtt
     5461 cctgccggag ggtttgagcg gcttcgtcgg caggaggagg ggataaatca tgagcggaca
     5521 gtggtttctt ttttccgtcg acctcctggc ccttttcggg atctacctcg ccctcaactt
     5581 gagccttcga cttcaactgg gctacacggg tatcgccaac ttcgggctgg tactcgcctt
     5641 caccggaggg gcctacgtcg gggggtggct ccctataagg cttggcatgt ggctggtcga
     5701 ggtggacccg tccctcgccg acgacatgat ctggaacaat gtcatcatcg cctcccaggt
     5761 ggacctttca ctccacggct cccccgggac cgcgatgacc atcctgctga tcaccctcct
     5821 ggcggcaggc gccgcagggg ctctcctggg cctcctgtcg ggttaccctg cggtcaggct
     5881 gggcggtgat tacctggcga tctcccttct cgccttcggc gaggtcctcg ccatcatcgg
     5941 caacaattac gaccccgtcg tggggggccc ccttggggtg aggatccccg acgtatgggc
     6001 ctgggcggga gactggaggt tcatggccgc gtccctggcg aattgcgctt tggcgctggg
     6061 ggtgtggctg tacctggact ccatcgaaaa atcccccatg gggaggatgc tgagggctat
     6121 cagggacaac cccgacgccg cctcctccct gggaaaaaac gtggccaggg tccgcttgaa
     6181 cgtcatggtg accggcggga tcatatgcgg gctcgccggg gcgatgtaca ccctttacac
     6241 gacgggggtg gcgccgagcg ccctcagcag gttcgactgg acctttct
//
LOCUS       DAOSRG010000048         6220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11589_length_6220_cov_9.341281, whole genome shotgun sequence.
ACCESSION   DAOSRG010000048 DAOSRG010000000
VERSION     DAOSRG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11589_length_6220_cov_9.341281"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLM30_02980"
     CDS             complement(<1..1149)
                     /locus_tag="PLM30_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR54167.1"
                     /translation="MDRDVKVILNGKEVFGYRGQKILELCAENGIEIPTLCYDPHLTL
                     HGGCSLCLVEVKGAKNLVRACAMPINNGMEIQTMNDRVFGARQLALQLLFSDHVGDCR
                     PPCQLACPARGDVKGYVNYAAEGEFRASLEALHENITLPASIGRICPAPCEEKCRRNF
                     VDEEPVSIREIKRFIGDWGIKTGDLGTIPEIKENGKKVAIVGGGPGGLSAAYYLRRMG
                     YAITLFDKESALGGMMRFGIPDYRLPQPVLQKEIDWIIDHGIETRLGVTLGKDVTLDE
                     LRKDYDSVLLAFGCWRSLPMRVPGEDLKGVLGGINFLYEVNNGRKVDVGDRVAVIGGG
                     NTAMDAARCALRSGAHKVYVVYRRTRDEMPAEKLEIDEAMEEDVEFIFL"
     gene            complement(1166..>2533)
                     /locus_tag="PLM30_02985"
     CDS             complement(1166..>2533)
                     /locus_tag="PLM30_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOR54168.1"
                     /translation="LAKVLLELPAEETLDIVKKSGLRGRGGAGFPTGRKWEFARMAPG
                     DKKYIVCNADEGDPGAFMDRSVLEGDPHSVVEGMLIGAYIIGSDEGYIYCRAEYPLAI
                     KRLNTAIEQAEELGLIGDNIMGTDFSFHLHIKEGAGAFVCGEETALMASIEGKRGMPN
                     PRPPFPANSGLWGKPTNINNVETWANIPQIILNGAEWFSSVGTETSKGTKIFALTGKV
                     NNTGLVEAPMGMTLREVIFDIGGGIPNNKKFKAVQLGGPSGGCLPESMLDTPVDYESI
                     NATGAIMGSGGMVVTDEDTCIVDFAKFFLTFVQNESCGKCPFCRIGTKRMLEILERIC
                     EGKGKLEDLDLLEDLAQKVKDGSLCALGGTAPNPVQTTIKYFRDEYIAHIVDKKCPAK
                     VCTALIHYTIDPDKCIGCTRCARECPVKAISGEVKKPHLIDQDLCIKCGRCMAVCPVK
                     AISVE"
     assembly_gap    2534..2633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2634..2797)
                     /locus_tag="PLM30_02990"
     CDS             complement(<2634..2797)
                     /locus_tag="PLM30_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR54169.1"
                     /translation="MALYRAHVLVCRGTGCTASGSGPLYETFKEELARRGLDKEVMLV
                     ETGCHGMCEMG"
     gene            complement(2810..3181)
                     /locus_tag="PLM30_02995"
     CDS             complement(2810..3181)
                     /locus_tag="PLM30_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR54170.1"
                     /translation="MKKITSLEDLRKIKEQSKDLTSARSGGKMRIIVGMGTCGIAAGA
                     REVMTAILQELEKRGLTDVAVETTGCIGMCQKEPLVDVIRPGEPRTTYGEVGPGDVPR
                     IIVEHIVNGNIVEDKVVGQTD"
     gene            complement(3159..3743)
                     /locus_tag="PLM30_03000"
     CDS             complement(3159..3743)
                     /locus_tag="PLM30_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54171.1"
                     /translation="MLQDLSQYILDIAENSLKAEASRVEIEVFEDTRGDLITLKVSDN
                     GYGMDDEQVSAIENPFFTTRTERRVGLGIPFLKQASDTCGGRFEIRSVKGRGTVVEAS
                     FKRDCIDCPPLGDIPATVMTLIVGWPERDFLFRFNFDDELFETGTEELVQVLQDRSLL
                     VTPEVALWISRYIEQGIYHLRTGGNEDFEENNQP"
     gene            complement(3752..4237)
                     /gene="nuoE"
                     /locus_tag="PLM30_03005"
     CDS             complement(3752..4237)
                     /gene="nuoE"
                     /locus_tag="PLM30_03005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOR54172.1"
                     /translation="MVVFVVRVQTADVEEKVKEIIEPWKDKKGSLIPILQNVQHEFGY
                     LREDVMEVISRELRIPMAEAYGVATFYAQFHLKPRGRHVIRVCRGTACHVRGSLKILD
                     RVKETLNVGEHETTDDLRFTLEPVACLGACGLAPVVMIDDTTYGRLTPEGIKPILDKY
                     E"
     assembly_gap    4520..4619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4620..4977)
                     /locus_tag="PLM30_03010"
     CDS             complement(<4620..4977)
                     /locus_tag="PLM30_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR54173.1"
                     /translation="MDLHIHTVLSPCAELDMGAPDIISCCKEKDIDLIAITDHNSALN
                     CEALNRASTGSGVEVICGLEVQSSEDIHVLCLMPDLVKARAFEEWVRKLLPPYRNIPD
                     KFGDQFVIDEDNHVIDE"
     gene            complement(5001..5339)
                     /locus_tag="PLM30_03015"
     CDS             complement(5001..5339)
                     /locus_tag="PLM30_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOR54174.1"
                     /translation="MKIRDILGNLEGVTIYVAAGEDVEITSGFVGDLLSFVMGSAPEK
                     ALWVTIQNHVNVAAVALLKEVPLVLLASGRVPSPELKDQCHKEGIALASIDMDSFEVC
                     GRLFALGVRN"
     gene            complement(5336..>6220)
                     /locus_tag="PLM30_03020"
     CDS             complement(5336..>6220)
                     /locus_tag="PLM30_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR54175.1"
                     /translation="LIQIRFPELIHNLSPAETPLETSVDLWRTRHNRYDIPSTLVAPC
                     PARITMAIRPVGRETSSIDNVVSTARVAKEILSKDCSPSRESTIPGRDSRWVHWSVSG
                     GEAGHIRMFEKRPLKTLAVSGLRNVVGILQEIELRRLKGVDFIEARMCDMGCIGGIAN
                     AESPFLSRLKVEDYPFAREIDKEEMAILEDLYQAGVWKLQQEILPIEQIPLGKDMAKA
                     MEKLSELQSVYAELPHLDCGTCGRPTCRVMAEDIVRGEASLDDCIFRLRKRMGDLSQE
                     LNELSKRFVHTMTPEGKE"
BASE COUNT         1174 a         1796 c         1642 g         1408 t
ORIGIN      
        1 caggaagatg aactccacgt cctcttccat ggcttcgtcg atctccagtt tttccgcggg
       61 catctcgtcg cgggtcctcc ggtagaccac gtagaccttg tgggctccgg agcggagcgc
      121 gcagcgggcc gcgtccatgg cggtgttgcc gccgccgatg acggcgaccc tgtctcctac
      181 gtcgaccttc ctcccgttgt tgacctcgta aaggaagttg atcccgccca ggaccccctt
      241 caggtcttcc ccgggcaccc tcatcggcag ggatctccag cagccaaagg ccaggagcac
      301 cgaatcgtaa tctttcctca gttcatcgag ggtcacgtcc ttgcccaggg tcacgcccag
      361 cctcgtttcg atcccgtggt cgatgatcca gtcgatctct ttctggagga ccggttgggg
      421 gagacggtag tcgggtatgc cgaatctcat catcccgccg agagccgatt ccttgtcgaa
      481 aagggtgatg gcgtaaccca tccttctcag gtagtaggct gcggagagac cgcccgggcc
      541 gccaccaacg atggccacct ttttcccgtt ctcctttatc tcggggatgg tgcccaggtc
      601 tccggtcttg atgccccaat ctcctatgaa ccgcttgatc tcacggatcg agaccggttc
      661 ctcgtcgaca aagttcctcc tgcacttttc ctcgcagggg gcgggacaga tacggcctat
      721 ggaggccgga agggtgatat tctcgtggag ggcttccagc gaagcccgga actccccctc
      781 ggcggcgtag ttgacgtagc ccttgacgtc gcccctggcg gggcaggcca gttggcacgg
      841 cggacggcag tcgcccacgt ggtccgagaa gagaagttgg agggccagct gcctggcgcc
      901 gaatacccgg tcgttcatgg tctggatctc catgccgttg ttgatgggca tggcgcaggc
      961 cctgaccagg ttcttagcgc ccttgacttc gacgaggcag agagaacacc cgccgtggag
     1021 ggtcaaatgc gggtcgtagc ataatgtggg tatctctatg ccgttctcgg cgcatagctc
     1081 caggatcttt tggccccgat acccgaaaac ctctttcccg ttaaggatta cttttacgtc
     1141 acgatccatt gtccaaagat cctccttatt caacgcttat cgccttgacc gggcatacgg
     1201 ccatgcatcg tccgcacttg atgcaaaggt cctggtcaat gagatgcggt ttctttacct
     1261 ctccgcttat cgccttgacc gggcattcgc gggcgcaccg ggtgcacccg atgcacttgt
     1321 cggggtcgat ggtgtagtgg ataagggctg tgcagacctt ggccgggcat tttttgtcga
     1381 cgatgtgcgc gatgtactca tcacggaagt acttgatagt tgtctggacc gggttgggag
     1441 ccgttccccc cagggcgcag agggacccgt ccttgacttt ttgggcgagg tcttccagca
     1501 gatccaggtc ttccaatttt cccttgcctt cgcagatccg ctccaggatc tccagcatcc
     1561 tcttcgttcc tatcctgcag aaggggcact tgccgcagga ttcgttctgg acgaaggtga
     1621 gaaagaactt cgcgaaatcc acgatgcagg tgtcctcgtc ggtgaccacc atgccaccgg
     1681 aacccatgat ggcgccggta gcgtttattg attcgtaatc gacgggggtg tcgagcattg
     1741 attcgggcaa gcagcctccc gaggggccgc ccagctggac ggctttgaat ttcttgttgt
     1801 ttgggatgcc gccgccgatg tcgaagatga cctccctgag ggtcatgccc atgggagcct
     1861 ccacgaggcc cgtattgttg accttaccgg tgagggcgaa gatcttcgtc cctttcgatg
     1921 tctccgtccc gacgctgctg aaccattccg cgccgttcag gattatctgc ggaatattag
     1981 cccaggtttc gacgttgttg atgttcgtcg gttttcccca gaggcccgaa ttggccggga
     2041 agggtgggcg ggggttgggc atgccccgct tgccctctat cgaggccatg agggcggttt
     2101 cctcgccgca gacgaaggcc ccggcgcctt cctttatatg gaggtggaag gagaaatcgg
     2161 tgcccatgat gttgtcgccg atgaggccca actcctcggc ctgttcaatg gcggtgttta
     2221 ggcgcttgat ggccaggggg tattcggcgc ggcagtagat gtacccttca tcggagccaa
     2281 taatgtaggc gccgatgagc atcccctcaa cgacgctgtg ggggtcgccc tccagtaccg
     2341 atcggtccat gaaagcccct gggtcgccct cgtcggcgtt gcacacgatg tacttcttgt
     2401 caccgggggc catcctggcg aactcccatt ttctgcccgt gggaaaaccg gcccctccgc
     2461 gtcccctgag gccggacttc ttcactatat cgagcgtctc ctcggccggc agttcgagaa
     2521 ggaccttggc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccattt
     2641 cgcacattcc gtggcaaccc gtctcgacaa gcatgacctc tttgtcgagc ccccgtcgag
     2701 ccagttcctc cttaaaggtt tcataaagcg gtcctgaacc gcttgcggtg cagcctgtcc
     2761 ccctgcatac cagaacgtgg gcgcgataca aagccatctt taaagccccc tagtcagttt
     2821 gtccgacaac cttgtcctcg acaatgttgc cgttgacgat gtgttcaaca atgatcctgg
     2881 gaacatctcc aggcccgact tcaccatagg tcgttcgggg ttcaccggga cggatcacgt
     2941 ccaccaacgg ttccttctgg cacatcccga tgcacccggt ggtctcaacg gctacatccg
     3001 tgaggcccct cttttcgagt tcctggagga tggcagtcat gacctccctc gcgccggcgg
     3061 cgataccgca ggtccccatt cccacgatga tcctcatctt tcccccggaa cgggccgaag
     3121 tgaggtcctt cgactgttcc ttgatcttcc tcaggtcctc aaggctggtt attttcttca
     3181 aaatcttcat tcccccctgt gcggagatgg tagatacctt gttcgatata gcgggagatc
     3241 cacaaagcga cctccggagt gactaatagt gacctgtctt gcaacacctg caccaactct
     3301 tccgtaccgg tctcgaaaag ctcatcgtcg aagttgaacc ggaacaaaaa atccctctcg
     3361 ggccatccca caatgagcgt catcaccgtg gcgggtatat ccccgagagg agggcagtcg
     3421 atgcaatctc ttttgaagct ggcttcaaca acggtacccc tacccttaac ggagcgtatt
     3481 tcgaatcggc ccccgcaggt atccgaggcc tgcttcagga aggggatacc gagcccgacc
     3541 cttcgttcgg tcctggtggt gaagaagggg ttttctatgg ccgatacctg ttcatcatcc
     3601 atgccgtaac cgttgtccga gaccttgagc gttatcaagt cccccctggt atcttcaaag
     3661 acctcgatct ctacccggga tgcttcggcc ttgagagagt tctctgcgat atcaagtatg
     3721 tattgggaga ggtcctgaag catcgcagct cttactcgta cttgtccagg atgggcttta
     3781 tcccttctgg agtaagcctt ccgtaagtcg tgtcatctat cattaccacg ggtgcgaggc
     3841 cacaggctcc aagacaggcg acaggctcga gggtaaagcg aagatcgtcg gtggtttcgt
     3901 gttcgcccac attaagggtc tccttgaccc tgtcaaggat cttgaggctt cccctgacat
     3961 ggcaggccgt gcccctgcac accctgatca cgtgtctccc cctgggctta aggtggaatt
     4021 gggcgtagaa ggtcgcgacg ccgtaggcct cagccatcgg gatcctgagt tccctggaaa
     4081 tgacctccat cacatcctca cggagataac cgaactcatg ctggacattt tgaaggatgg
     4141 gtatcaggct accctttttg tccttccagg gttcaattat ttccttgacc ttttcctcga
     4201 catccgcagt ctggaccctt accacaaaga ccaccacctg gatgaaattt tgaggttcga
     4261 aaataccata aagccaagtg aattgtatca ctttatagat aaattgcaaa cattttcgaa
     4321 aaggagtcac ttcgaaaaac aaagcgctta gggcccgatc cgcctcattt cccgtaggaa
     4381 ctcaggggct attacggtcc cagggccaga gcacctttcg tccgtcgacg cccttgagcg
     4441 cgagggacag ttcatggaag gatggtttcc ccagtagcgc catggtggtc ctttcttggc
     4501 cgatatcctt cagggtatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4621 ttcatcgatg acatggttgt cctcgtcgat cacgaactgg tccccgaatt tgtcgggaat
     4681 gttccgataa ggggggagca gtttcctgac ccattcctcg aaagcgcggg ccttcacgag
     4741 atcaggcata aggcagagga catgaatatc ttcagaggac tggacctcca acccgcagat
     4801 gacctcaacg cccgaacctg tcgaggccct gttaagggcc tcgcaattca gagcggagtt
     4861 gtggtcggtt atcgcgatca ggtcgatgtc cttttccttg caacaggaaa taatgtcggg
     4921 cgcccccata tccagttcgg cgcacgggga gaggacggta tggatgtgca gatccatgcg
     4981 atagagatcc atgggctggg tcagttccta acccccaagg cgaaaagcct tccacagacc
     5041 tcaaaggagt ccatgtctat agaggccagg gcgatccctt ccttgtggca ctggtccttc
     5101 aattccggcg atggcactct tcccgacgcc aggaggacca gggggacctc cttcaacagc
     5161 gcgacggcgg ccacatttac atggttctgg atggtcaccc acaacgcttt ttcgggcgcc
     5221 gagcccatta caaaactaag caggtcccct acaaacccac tggtgatctc cacatcttcg
     5281 ccggcggcca cgtagatagt gaccccctcc aggtttccca gaatgtcccg tattttcatt
     5341 ccttcccctc cggggtcatg gtatggacaa atcttttaga aagttcgttg agttcctgtg
     5401 acaggtcgcc catccttttc ctcagcctga agatgcaatc atccaacgaa gcttcaccgc
     5461 ggactatatc ctcggccatc accctgcagg tcggtcggcc gcaggtcccg cagtcgaggt
     5521 ggggtaattc ggcatagacc gactggagtt ccgaaagttt ttccatggcc ttcgccatat
     5581 ctttgccaag ggggatctgt tcgatcggga ggatctcctg ctgcagtttc caaacgccag
     5641 cctggtaaag atcctccagg atcgccattt cttccttgtc gatctccctg gcaaaggggt
     5701 aatcctctac tttgagtctg ctgaggaagg gggattcggc gttggctatc cccccaatgc
     5761 agcccatatc gcacatcctg gcctcgataa aatcgacccc cttgagcctc cgtagctcga
     5821 tttcctgcaa gatcccaacg acattcctca aaccggaaac agcgagggtc ttcaagggtc
     5881 tcttctcgaa catcctgata tgaccggcct caccgccgga cacggaccag tgtacccaac
     5941 gggaatccct tcccggaatg gtcgattccc tggaagggga acagtccttc gacagtatct
     6001 ccttcgccac cctggccgtg gagaccacgt tgtctatgga actggtctcc ctccccacgg
     6061 gccttatagc catggtgatc cgcgcggggc acggggccac cagggtggag gggatgtcgt
     6121 acctgttatg acgggtcctc caaaggtcga cgctggtctc caggggggtt tcggcgggcg
     6181 aaaggttgtg tatcagctcc gggaacctga tctgtatcag
//
LOCUS       DAOSRG010000049         6202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11623_length_6202_cov_8.166423, whole genome shotgun sequence.
ACCESSION   DAOSRG010000049 DAOSRG010000000
VERSION     DAOSRG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11623_length_6202_cov_8.166423"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2947)
                     /locus_tag="PLM30_03025"
     CDS             complement(<1..2947)
                     /locus_tag="PLM30_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR54176.1"
                     /translation="MKLPEISVKRPVMTLMVFLAVLVIGSISFRSLKLDLLPRIEPPV
                     ITVITPWPGASASDVEQRITKVMENSLSMLEGVDDLISRSLDNISVVSVKFKWGVDLD
                     VRAGDVRDAVNFAKRELPSDAEESVVLRITSGTVPVVEVALTAERSYQGLYHFVDKNV
                     VEELSRVPGVGQVLIFGGVAREIQVLLDADRLEAYGLSPDAIAGVLERENLNIPSGSM
                     KEGRTEYFIRVPGRFTSVEEIRNTVVGIVKGNAVRVGDVAEVRDGYKELTMNGWKDDQ
                     AAVVMIVMKNSDANTIEVSRAVLDRLSRLKEESFPSDVKYDVVLDTADFIMNSITNLS
                     VSLVVGIILVFLVTWAFLRRFRASLIVAGAIPFSLILTFIAMGRLDYTINIFTLSALA
                     MASGMVVDNAIVATDQIIYHIEQGSRRRVAAILGAGEIGSALLASTLTTIVVLLPLAF
                     LSGLVGVFFSALTVVMVLAVSASLFVALTFIPMMGSKFFGREPGKLKIHQVTEKFFLW
                     LEDRYSAMLAWGLDNRKKVIASALLLLVLTFLGFRTIGTELTPDPDTGDISITFSLPE
                     GTRLEVTDELVREIMDYCRRTIPEAKLVFGMDGMEEGGLSVAVGQKAGPNTGTIGIRL
                     VDKNKRDRSAFEVAGVIRDWIRAKPGIEDMTVFVSSPIKAMFLGSKPLDIEIYGDDLR
                     QVTKASSMIASGLADIPGTADISVSRREDRPELWIEPDREKAAYLGVSAAAVSRTARI
                     YFAGYETEGSFWEGEDDYPIRVRLREEQRNDRDALGRLTVPSASGGLVRLSSVTAMKD
                     EVGPPQIERKNRQRYLTVGANVHGRSLGEVTADAVKMVQSMEIPEGVRVTFGGQIKEQ
                     QDAFRQMGLLVLLGILLVYMVMAGQYEAYLDPFVILFSIPFALTGVVFAFLVTGLYIS
                     LQALLGIIMLVGIVVNIAIVLVDYINLVRARGSLLRDAVIEAGRRRLRPALMTTLTTF
                     FGMLPMALSR"
     gene            complement(2944..3990)
                     /locus_tag="PLM30_03030"
     CDS             complement(2944..3990)
                     /locus_tag="PLM30_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR54177.1"
                     /translation="MKKTFVLVVIVALFAGLILFRGHQQKQLKTATPEEEIARVETVR
                     LREMDLAEVVEFTATVEPEEQSAVVPKVPGRTVLEVFVSEGDRVKKGQPLASLDASLI
                     RRQLEEARAFYETAAADYERYRSLYDEEVVSRQAVDQARARYAQAKSAYEQAGIMSGY
                     HTITAPAGGVVARRFIDPGDTAPQGPAFLIFRQDNVKAVGAVPERAFTGIRLGAKVTL
                     LVDAVPDREFEAKVTNLSPVIDPATRTGKIEVSLPSEGVILPGMFVRARMTAGERRVM
                     ALPREAVSRLAGTGETVCYVAEGGKAILRVIGTGIEHEGFVEITEGLTPGDEVITTRA
                     GTVRDGTRIEVYRR"
     gene            complement(3996..5345)
                     /locus_tag="PLM30_03035"
     CDS             complement(3996..5345)
                     /locus_tag="PLM30_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR54178.1"
                     /translation="MMQQRRIIAITFIALSLFVIFLGRRAWADGKPVLDEGLAVVLAL
                     ENNPQIGIAEKRVEQAEARVREASSGKAPVLSASALYQKTFHEPQYPLVTNDPSLTGY
                     ANAGFRETWKTALSLSWLVYSGGAVESGIKAAGFAAEAAGAEAQRIAQAVAHGAKSAF
                     FELQRARARAVVAGEARRLALDHLANVEALYRNGVVAKNELLRAEVAVSDAKLNLIRA
                     ESGIEVAFSALERAVGTAVKDKYSLPEPQEEPSPVEMPGDPLGAALAGRPEFRALESS
                     VRSAKAMAAAAQGEKGPSVFLQGEAFSVGQTFYPDAADDWKVSVVAQWTLYDGGKAKA
                     RRDEALALAGELLQRVEDLKKSVGLEVSVATLDLRSALQRLEVARSQVALAEEDHRMA
                     VARYAAQVGTNIDVHQARVALTLSRTQLVDAVYDALKAQSDMELAMGEEVRIPGDER"
     gene            complement(5342..5944)
                     /locus_tag="PLM30_03040"
     CDS             complement(5342..5944)
                     /locus_tag="PLM30_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR54179.1"
                     /translation="MSDPCGTRDRILAVARSQFAQNGYHGTSMDAIVKETGLSKGALY
                     WHFEGKSDLFAAVLAREVDRMIHSHVEASRGEGQDVRAFVTLRGERTIEMLWEDREMR
                     LLFFTLFIERLRGGEEGRELSQVAGDLLEVIQEKLWPVAREVFPAFRHREAYQKMLTT
                     LRFLMNGIIMELGFSLDLDSAREYWRFAIGRFEFQSEGFK"
BASE COUNT         1050 a         1997 c         2010 g         1145 t
ORIGIN      
        1 ccctgctcag ggccatgggg agcatgccga agaaggtggt gagggtcgtc atgagggcgg
       61 gcctgaggcg ccggcgcccc gcctcgatga cggcatccct gagcaacgac ccgcgggccc
      121 tgacgaggtt tatatagtcc accaggacga tggctatgtt gacgacgatg cccacgagca
      181 tgatgatccc cagcagggcc tgcagggaga tgtagagccc cgtcacgagg aaggcgaaga
      241 ccacccccgt gagggcgaag gggatgctga agagtatgac gaaggggtcg aggtacgcct
      301 cgtactggcc cgccatgacc atgtagacga gcaatatgcc cagcaggacc aggagcccca
      361 tctgccggaa ggcgtcctgc tgctccttta tctggccccc gaaggtcacc ctcacgccct
      421 cggggatctc catggactgg accatcttga cggcgtcggc ggtgacctcc cccagggagc
      481 gcccgtggac gttggctccc acggtcaggt agcgctgccg gtttttcctc tctatctggg
      541 gcggcccgac ttcgtccttc atcgcggtca ccgacgacag ccggacgagt cctcccgagg
      601 cggaggggac ggtcagcctg ccgagggcgt ccctgtcgtt ccgctgctcc tctctgagcc
      661 tcactctgat ggggtagtca tcctcgccct cccagaagga gccctccgtc tcgtagccgg
      721 cgaagtagat cctggccgtg cgggagaccg cggcggcgct gacgcccagg taggccgcct
      781 tctcccggtc cggttcgatc cagagttcgg gccggtcctc ccggcggctg accgatatgt
      841 cggccgtgcc cggaatatcg gcgaggcccg aagcgatcat ggaggacgcc ttcgtcacct
      901 gcctgaggtc atcgccgtag atctcgatgt ccaggggctt ggagccgagg aacatggcct
      961 ttatgggcga actcacgaag accgtcatgt cctcgatgcc gggcttggcc cgtatccagt
     1021 cgcggatgac gccggccacc tcgaaggcgg agcggtcccg cttgttcttg tccacgagcc
     1081 tgatgcctat cgtgccggtg ttggggcccg ctttctggcc gacggccacg gagaggcccc
     1141 cctcctccat cccgtccatc ccgaagacga gtttggcctc gggaatggtc cgccggcagt
     1201 agtccatgat ctcccggacc agttcgtcgg tcacctcgag cctggtgccc tcggggaggg
     1261 agaaggtgat ggagatgtcg cccgtgtcgg ggtcgggggt cagttcggtg ccgatggtcc
     1321 ggaaaccgag gaaggttagc accagcagca ggagggccga ggcgatgacc tttttgcggt
     1381 tgtccagccc ccaggccagc atggccgagt accggtcctc gagccagagg aagaattttt
     1441 ccgtcacctg gtgtatcttc agttttcccg gctcccggcc gaagaacttg cttcccatca
     1501 tgggtatgaa ggtgagggcc acgaagagcg aggccgagac cgccaggacc atgaccaccg
     1561 tgagggccga gaagaagacg cccacgaggc cggaaaggaa ggccagcggg aggaggacga
     1621 cgatggtggt gagcgtcgag gccagcaggg ccgagcctat ctccccggcg ccgaggatgg
     1681 ccgcgacgcg ccgccggctg ccctgttcta tgtggtagat gatctggtcc gtggcgacga
     1741 tggcgttgtc caccaccatg ccgctggcca tggccagggc cgagagggtg aagatgttta
     1801 tcgtgtagtc gaggcgcccc atggcgatga aggtaaggat cagcgagaag ggtatggccc
     1861 ccgccactat cagggaggcg cggaacctcc tcaggaaggc ccaggtcacg aggaagacca
     1921 ggattatccc taccacgagg gagacgctga ggttggtgat ggagttcatg atgaagtcgg
     1981 cggtgtccag gaccacgtca tacttcacgt cggaggggaa gctctcctcc ttgaggcggg
     2041 agagcctgtc caggaccgcc cgggagacct cgatggtgtt ggcgtcgctg ttcttcatca
     2101 cgatcatcac gacggcggcc tggtcgtcct tccacccgtt catggtcagt tccttgtagc
     2161 cgtccctgac ctcggcgacg tcgcccaccc tgacagcgtt gcccttcacg atgcccacga
     2221 cggtgttcct gatctcttcc accgaggtga accggccggg caccctgatg aagtattccg
     2281 tgcgcccctc cttcatggag cccgagggga tgttgaggtt ctccctctcg agcacgcccg
     2341 cgatggcgtc gggagacagc ccgtaggcct caagcctgtc ggcgtcgagg aggacctgga
     2401 tctccctggc gacgcccccg aagatcagga cctgacccac tcccggaacc ctggagagtt
     2461 cctcgacgac gttcttgtcg acgaagtggt agagcccctg gtaggacctc tcggccgtca
     2521 gggcgacctc cacgacgggg accgtcccgg aggttatcct gagcaccacc gattcctcgg
     2581 cgtcggaggg gagttccctc ttggcgaagt tgacggcgtc cctcacgtcc ccggcccgga
     2641 cgtcgaggtc cacgccccat ttgaacttga ccgagacgac ggagatgttg tccagggacc
     2701 gggagatgag gtcgtccacc ccttcgagca tggagaggct gttctccatg accttggtta
     2761 tcctctgctc cacgtcgctg gcggaggccc ccggccacgg tgtgatcacc gtaatgacgg
     2821 ggggttcgat cctgggcagg aggtccagtt tcaggctgcg gaaggatatg gaaccgatga
     2881 ccaggacggc gaggaagacc atcagggtca tcacggggcg cttgacggag atctcgggca
     2941 gtttcacctg cggtacacct caatcctggt gccgtccctg acggtgccgg ccctggtagt
     3001 gatcacttcg tcgccgggtg tgagcccttc ggtgatctcc acgaagccct cgtgctcgat
     3061 gcccgttcct atgacgcgga ggatggcctt gccaccctca gccacgtagc ataccgtctc
     3121 gcccgtgccg gcgaggcggc tgacggcctc cctggggagg gccatgaccc gtctctcacc
     3181 ggcggtcatc ctggcccgga cgaacatccc ggggaggatc accccctccg aggggaggga
     3241 aacctcgatc ttccccgtgc gggtggcggg gtcgatgacg ggggagaggt tcgtcacctt
     3301 ggcctcgaat tccctgtccg ggacagcgtc caccagcagg gtgaccttgg caccgaggcg
     3361 gatcccggtg aaggcccttt cggggacggc ccccacggcc ttgacgttgt cctgcctgaa
     3421 gatgaggaag gccggcccct ggggggcggt gtcgccgggg tctatgaagc gcctggccac
     3481 gacgcccccg gccggggctg ttatggtgtg gtaaccgctc atgatcccgg cctgttcgta
     3541 ggccgacttg gcctgggcgt agcgggccct cgcctggtcg acggcctggc ggctgaccac
     3601 ttcctcatcg tagagggacc ggtagcgctc gtagtcggca gcggcggtct cgtaaaaggc
     3661 gcgggcctcc tcgagctggc gccggatgag gctggcgtcg agggatgcca ggggctggcc
     3721 ctttttcacc ctgtcgcctt cactgacgaa gacctccagg acggtcctcc ccggaacttt
     3781 gggcactacg gcggactgct cctcgggctc tacggtggcg gtgaactcca ccacctcggc
     3841 gaggtccatc tcacgcaggc ggaccgtttc gaccctggcg atctcttcct caggcgtggc
     3901 ggttttcaac tgcttctgct ggtgccccct gaaaaggatg agccccgcga agagcgccac
     3961 gatcactaca agaacgaatg tctttttcat gcccatcacc tttcgtcccc ggggatacgg
     4021 acttcctcgc ccatggccag ttccatgtcc gactgggctt tcaaggcgtc gtagacggca
     4081 tcgaccagct gggtcctcga cagggtgagg gccaccctgg cctggtgcac gtcgatgttg
     4141 gtgcccacct gggccgcgta cctggccacg gccatgcggt ggtcctcctc ggccagggcc
     4201 acctgggacc gggccacctc gagccgctgg agggccgaac ggaggtccag cgtcgcgacg
     4261 gaaacttcga gccccacgct tttcttcagg tcctccaccc gctgcagcag ttcgccggcc
     4321 agcgccaggg cttcgtcccg gcgggccttg gccttgccgc cgtcgtaaag ggtccactgg
     4381 gctaccaccg ataccttcca gtcgtcggcg gcgtcggggt agaaggtctg ccccacggaa
     4441 aaggcctccc cctggaggaa gaccgagggg cccttctccc cctgggccgc ggcggccatg
     4501 gccttcgccg agcgcaccga cgactcgagg gccctgaact cggggcgccc cgccagggcc
     4561 gctccgaggg ggtcgccggg catttccacc ggcgagggct cctcctgggg ctcggggagg
     4621 gagtacttat ccttgacggc cgtcccgaca gccctctcga gggcgctgaa ggctacctcg
     4681 atgccgcttt cggcccggat gaggttcagc ttcgcgtcag agaccgccac ctcagccctg
     4741 agcagttcgt tcttggccac cacgccgttc ctgtaaaggg cttcgacgtt cgccaggtga
     4801 tccagggcaa gccgcctcgc ctccccggcg acgacggccc gggcccgggc cctctgcaat
     4861 tcgaaaaagg cgcttttcgc gccgtgggcc acggcctggg cgatcctctg cgcctcggct
     4921 ccggcggcct cggcggcgaa cccggcggcc ttgatgccgc tttcgacggc gccgccgctg
     4981 tagacgagcc aggagaggga gagggccgtc ttccaggtct cgcggaaacc ggcgttggcg
     5041 tagcccgtga gggacgggtc attcgtcacc agtgggtatt gcggttcatg gaaggtcttt
     5101 tgatagagcg ccgaggccga aaggacgggc gctttccccg aggaagcctc cctgaccctg
     5161 gcctcggcct gctcgaccct cttttcggct atgcctatct gggggttgtt ctccagggcg
     5221 aggaccacgg cgaggccctc gtccagcacg ggcttcccgt cggcccaggc cctcctgccc
     5281 aggaatatca cgaagaggga gagcgcgatg aacgtgatgg ctatgatcct tctctgctgc
     5341 atcatttgaa cccctcgctt tgaaattcga aacgcccgat ggcgaagcgc caatactccc
     5401 tggccgagtc caggtccagg ctgaacccca gttccatgat tatcccgttc atcaggaaac
     5461 ggagggtggt cagcattttc tggtaagcct cgcggtgtct gaaggcggga aagacttccc
     5521 gggccacggg ccagagcttc tcctggatga cctccaggag gtcgcccgcc acttgggaca
     5581 gttccctgcc ctcctccccc ccccgaagcc tctcgatgaa gagcgtgaag aagaggaggc
     5641 gcatttcccg gtcttcccag agcatctcta tggtccgctc cccccggagg gtgacgaagg
     5701 cgcggacatc ctgcccctcg ccgcgggatg cctcgacgtg gctgtggatc atcctgtcga
     5761 cctcacgggc aaggacggcg gcgaagaggt cgctcttgcc ttcgaagtgc caatagaggg
     5821 cccccttgct gaggccggtc tccttgacga tggcgtccat gctggtgccg tgataaccgt
     5881 tttgggcgaa ttgactcctg gccaccgcca ggatcctgtc cctcgtcccg caggggtcgg
     5941 acattgtatc gcctccagac cgtacggttt gtgcaacagg ctgcaatcta tcacagaccg
     6001 accggtctgt caagggaaaa agataaaagc cgtaaatcgt gaatcggaag ggccgtaaat
     6061 cgtaaatcgg gaatcgtgaa tcgtaaatcg caaagaccgt gaatcgaaag atcaaattcg
     6121 caggatgatc gtttcactct gacgatcagt caagtcccag aactgctgta aattcggatc
     6181 cggttacata ttcaccgcca cg
//
LOCUS       DAOSRG010000050         6189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11655_length_6189_cov_9.762798, whole genome shotgun sequence.
ACCESSION   DAOSRG010000050 DAOSRG010000000
VERSION     DAOSRG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11655_length_6189_cov_9.762798"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1115
                     /gene="ltaE"
                     /locus_tag="PLM30_03045"
     CDS             81..1115
                     /gene="ltaE"
                     /locus_tag="PLM30_03045"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOR54180.1"
                     /translation="MKVIDLRSDTVTKPTDEMRAAMAAADVGDDVYGEDPTINELEEE
                     SALMTGQEAALFVTSGTQGNVAALLTHCSRGDGAILGSDSHIYIYEGGGLSALGGILP
                     LVADDPGGIIPPQKVLSWCRPSNVHFAPARLLCVENTHNRAGGTAVSPKDFKATVEAA
                     RSGGLSVHLDGARVFNASTAWGVDVKEYTGIVDSVQFCLSKGLGAPVGSMLCGGAEFI
                     SRARHWRKRLGGGLRQAGILAAAGLIALRKMTKRLAEDHENAARLSSALLAGGLGVER
                     VGKPTNMVYVSLGSDGPDANELSRRCAAGGVLFGAVSERRFRLVTHFGVAAGDVDRAA
                     ETIMEELKAS"
     gene            1115..2479
                     /locus_tag="PLM30_03050"
     CDS             1115..2479
                     /locus_tag="PLM30_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR54181.1"
                     /translation="MSQGPSRQEVESIARWMMEASLDRGALLADVLYRSGRSSRLSLR
                     DGDPEWDTLGSSIRIGCRVADREGRQGVADTSDLSRDAIRQLVDLCLENCRASEPEVD
                     VALYEGKAPDTPETGVFDADTAGMGHTEREERCRLMHDLASRADNRVVSVRETSWSDG
                     SESTFYASSEGFLGWYDETWASCAVTVVLSDGEAQEMSGAYAESRRASDIDPGSVARE
                     AVERGALTLGGKPAPTGVYDVVLDPEAAASLVEAVGELFLAPEVFKNRSMLKGRVGQK
                     VGSECLTLVDDGTIPWAAGTSPWDGEGVPPGRTVLLDRGTVASFLYDLKYAKKFGTYS
                     TGNASRSPGSLPDVGFSNLFPVPGKRGKGQLLKDCAGGVFVTELMGVHTIDPISGDLS
                     LGVKGALIDGSGRPGSPVAGMTMAGNLLSLLSALSEVGDDLRFFGEIGGCTMVVRNVS
                     MAGA"
     gene            2457..4121
                     /locus_tag="PLM30_03055"
     CDS             2457..4121
                     /locus_tag="PLM30_03055"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR54182.1"
                     /translation="MSRWPAPRAALGAIAALLFLAVLSPGALSAPVEWELLRDGRALG
                     RVPVMDAQGGRLVALDSAAGLTGLSTEAKGDTLVLKGPGGSLQLFPGAAAAVHKGEII
                     PLMHEVVREGGHWWVDPDCALVLCGRVAGPEGGSLAWKGEGYKPRPQPLPGPPPGSST
                     PPVQAHKDPLSAAPATVRSIRWGRQDFGIRVVLDLSNPTAPKVEAGPGAVTVTIPGLM
                     ARGTAGSVSPYPSEVGLSVTQFGDRVVLSFKHNATSVRHFALEGPCRQVIDFYDPMPV
                     GMAPAPYAPWTGPPEEVTVEEEAVEVEEELSAEAGPALPAPDMPVKAPGGARTVVIDA
                     GHGGKDPGAVANGVREKDINLRVARLMADMLKKEGLRVVLTRSTDVYLQLSQRTSIAI
                     KEDADAFVSLHCNALPKGRSAQGMEIYLMALPSDKHAMELALIENRELVGGGLESSEA
                     VDRRTRTLLKILGDMQQNVKIQESTSFAEILYRRGGAKKLTMRRVAQAPFFVLRGAGM
                     PSVLVEMGFLTNKAEAAMLKNANYQKRIAEALSAGIVEFLGQSEFN"
     gene            4172..4858
                     /locus_tag="PLM30_03060"
     CDS             4172..4858
                     /locus_tag="PLM30_03060"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOR54183.1"
                     /translation="MRESQDWEKRGPKPLFDDDDEDELYDDEEGGRVHPALRVVAWVS
                     VLVLLFAVGYWGTSLTLKYLNRKEIITQRNVVSDPAEAKKVLEDTSEPSGLSVKRSGF
                     EVFVPRGGLLEPETVSLVPGILEEDVKTVILGLMETMRSENTITEQVRVLHVFRNGDL
                     LYLDLNDPFVKVVQKLTAEKATLIMTSLVRTIVANFSPVTRVRILINGKDPELKTPVD
                     LTTPWQLKTS"
     gene            4876..5664
                     /gene="rph"
                     /locus_tag="PLM30_03065"
     CDS             4876..5664
                     /gene="rph"
                     /locus_tag="PLM30_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOR54184.1"
                     /translation="MPGKRADGRDPGEHRPVNMTRGYSRFAEGSALVEFGDTRVLCTA
                     SVEERVPLFMRGGGKGWVTAEYSMLPRSTETRTPRDSVRGGVSGRSHEIQRLIGRSLR
                     AGTFLELLGERTVWVDCDVLQADGGTRTAAITGGFVALADALRWLFARGVIDTIPLKC
                     FVAAISVGKVDGSLVTDLSYGEDSRAEVDFNIVMNEFGDFVEVQGTAEGDPYSRSELL
                     NMIGLAERGVASMISVQKSALSLSGEEERAVALARDRIRQRQQG"
     gene            5627..>6189
                     /locus_tag="PLM30_03070"
     CDS             5627..>6189
                     /locus_tag="PLM30_03070"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOR54185.1"
                     /translation="MPGIVFASGNRDKYLEMRSLLAPMGVELLFGPDLAGSPGLPAVE
                     EDRDTYAGNAIVKALTWAMALGRSALADDSGLEVKALGWGPGVMSARVAPDDRARVEW
                     LLERMRGAVEREARFVAALALYEIDRGRWFLAEGFCYGRIAPAPSGRYGFGYDPLFIP
                     RGYDETLAVLGPEVKSRLSHRSVAAGAL"
BASE COUNT         1060 a         1992 c         2148 g          989 t
ORIGIN      
        1 tttgaaggat cggaatgatg ctattataac aagcaaagaa aaggccgccg cctaaagccc
       61 ggccggggag gctgaagccc atgaaggtta tcgatcttcg cagcgatact gttacaaagc
      121 ccaccgatga aatgagggca gccatggccg ctgctgacgt gggggacgac gtttatggcg
      181 aggaccccac gataaacgaa ctggaagagg aatccgccct tatgacggga caggaggccg
      241 cccttttcgt gacctccgga acccagggca acgtcgccgc cctcctgacc cactgctccc
      301 ggggcgacgg ggccatcctc ggctccgaca gccacatcta catctacgaa ggcggggggc
      361 tctccgccct cggcggcatc ctgcccctgg tggccgatga tcccgggggg atcataccgc
      421 cccaaaaggt cctctcctgg tgcaggccga gcaacgtcca tttcgccccg gcccgcctcc
      481 tttgtgtgga gaacacccac aacagggcgg ggggtacggc cgtatcgccg aaggatttta
      541 aggccaccgt ggaagcggcc cggagcgggg gactttcggt gcaccttgac ggcgccaggg
      601 tcttcaacgc ctccaccgcc tggggcgtcg atgtgaagga gtacacgggg atcgtggact
      661 ccgtccagtt ctgcctttcg aaggggctgg gcgcccccgt cggttccatg ctctgcggcg
      721 gcgccgagtt catatccagg gcaaggcact ggcgcaagag gctcggcggc ggcctgaggc
      781 aggccgggat cctggccgcg gcggggctca tagccctgcg gaagatgacg aagaggctcg
      841 ccgaggacca cgagaacgcc gccaggctgt cgtcggcgct ccttgccggc ggcctcggtg
      901 tggaaagggt gggcaagccc accaacatgg tctacgtcag cctggggtcc gacgggcccg
      961 acgcgaacga actgtcccgg cggtgcgccg ccgggggcgt cctcttcggg gccgtctccg
     1021 agaggaggtt caggctggtg acccacttcg gggtcgccgc cggcgatgtg gaccgggccg
     1081 ccgaaaccat aatggaggag ttgaaggcct cctgatgagt caaggccctt cccggcagga
     1141 ggttgaatcg atagcccgct ggatgatgga ggcctccctt gacagggggg cgctcctggc
     1201 cgatgtgctc taccggtcgg ggaggtcctc gaggctctcc ctcagggacg gtgaccccga
     1261 gtgggacacc ctggggtcct cgatccggat aggctgccgc gtcgccgaca gggaagggcg
     1321 ccagggcgtg gctgacacga gcgacctctc tcgggacgcg atccggcagt tggtcgacct
     1381 gtgcctcgag aattgccgcg cctccgaacc cgaggtcgac gtggccctct acgagggaaa
     1441 ggcccccgat accccggaga ccggtgtctt cgatgccgac accgccggca tgggtcacac
     1501 cgagagggag gagcgctgcc gcctgatgca cgacctggcg tcccgcgccg acaacagggt
     1561 cgtctccgtc cgggagacgt cctggtcgga cggttcggag agcacctttt atgcctccag
     1621 cgagggcttc ctggggtggt acgatgagac ctgggcctcc tgcgccgtga cggtggtcct
     1681 ctccgacggc gaggcccagg agatgtccgg ggcctacgcc gagagcaggc gggcttccga
     1741 catcgacccc gggtccgtgg cgcgggaggc tgtcgagagg ggcgccctta cgctgggagg
     1801 aaagccggcc cccaccggtg tctatgacgt ggtgctggac cccgaggccg ccgcttccct
     1861 tgtggaagcc gtgggggaac tcttcctggc gcccgaggtc ttcaagaacc gatcgatgct
     1921 gaagggtcgg gtcggacaga aggtcggaag cgagtgcctc accctggtgg acgacggaac
     1981 catcccctgg gcggccggta catcgccctg ggatggggag ggggttcctc cgggccggac
     2041 ggtgctgctg gaccggggga ccgtggcctc cttcctctac gacctcaagt acgcgaagaa
     2101 gttcgggacc tactccacgg ggaacgcttc cagatccccc gggagcctcc ccgacgtggg
     2161 tttttcgaac cttttccccg tccccgggaa gagggggaaa gggcagttat tgaaggactg
     2221 cgccggcggg gtcttcgtga cggaactcat gggcgtccac accatcgacc ccatcagcgg
     2281 tgatctctcc ctgggggtca agggtgcgct gatcgacggt tccggccgcc ccgggagccc
     2341 cgtggcgggc atgaccatgg ccggcaacct tttgagcctt ctttcggccc tttccgaggt
     2401 gggcgacgac ctgcgcttct tcggcgagat cggggggtgc accatggtgg ttcggaatgt
     2461 ctcgatggcc ggcgcctagg gcggccctcg gcgccatagc ggcgctcctg ttcctggctg
     2521 tcctttcacc gggcgccctc tccgccccgg tagagtggga actgcttcgc gacggccggg
     2581 ccctcgggcg ggtccccgtg atggacgccc agggcggcag gctggtggcc ctggactccg
     2641 cagcggggct cacgggcctc tccaccgagg cgaagggcga taccctggtc cttaaaggcc
     2701 ccggggggtc gctgcagctt tttcccggcg cggcggcggc cgtccacaag ggggagatca
     2761 tccccctgat gcacgaggtg gtgcgcgaag gcgggcactg gtgggtggac cccgactgcg
     2821 ccctcgtcct ttgcggccgc gtggcgggcc ccgagggcgg ctccctcgcc tggaagggtg
     2881 agggctacaa gcccaggccc cagcctctcc ccggcccccc gccgggcagc tccacaccgc
     2941 cggtccaggc gcacaaggat cctctttccg ccgcgccggc cacggtgagg tccatccggt
     3001 ggggacggca ggatttcggc atcagggtcg tcctggacct ttcgaacccc acggccccga
     3061 aggtcgaggc cgggcccggg gcggtcacgg tgaccatccc cggcctgatg gccaggggta
     3121 cggccggttc ggtctcgccc tacccctcgg aggtcggcct ctcggtgacc cagttcggcg
     3181 accgcgtcgt cctgtccttc aagcacaacg ccacgtcggt gaggcacttt gccctcgagg
     3241 gcccctgcag gcaggtcatc gacttctacg acccgatgcc cgtcgggatg gcgccggcgc
     3301 cctacgcccc ctggacgggg ccgcccgaag aggttaccgt agaagaagaa gcagtggaag
     3361 ttgaagaaga actctccgcg gaggcgggcc cggccctccc cgcaccggac atgccggtca
     3421 aggcccccgg cggagccagg accgtggtga tcgacgccgg gcacggcggc aaggaccccg
     3481 gggcggtcgc caacggggtc agggagaagg acataaacct ccgggtggca aggctcatgg
     3541 cggacatgct caagaaggag gggctccggg tggtcctcac caggagcacc gatgtctacc
     3601 tccagctgtc gcagaggacg agcatagcca taaaggagga cgccgacgcc tttgtgagcc
     3661 tgcactgcaa cgcgctcccc aagggcaggt ccgcccaggg gatggagata tacctcatgg
     3721 ccctgcccag cgacaagcac gccatggaac tggccctgat agagaacagg gaactcgtcg
     3781 gcggcggtct tgagagcagc gaggccgtcg acaggagaac acggaccctg ctgaagatcc
     3841 tcggcgacat gcagcagaac gtaaagatcc aggagagcac ctccttcgcg gagatcctct
     3901 accgcagggg aggggctaaa aagctcacca tgaggagggt cgcccaggcc cccttcttcg
     3961 tgctccgggg ggcggggatg ccatcggtcc tggtcgagat gggtttcctg accaacaagg
     4021 ccgaggcggc gatgctcaag aacgccaact accagaagcg gatcgccgag gccctttccg
     4081 ctggtatcgt ggaattcctg ggacagagcg aattcaatta ggcgccgccc ggcatccttt
     4141 taaagaccaa ttgcgatcgg aggggagaaa catgcgggaa tcccaggatt gggagaagag
     4201 gggcccaaag cctcttttcg atgatgacga tgaagacgaa ctctacgacg acgaggaggg
     4261 cgggagggtc cacccggccc ttcgggtcgt cgcctgggtc tccgtcctgg tgctcctttt
     4321 cgccgtgggg tactggggaa cgtcgctgac cctgaagtac ctgaaccgga aggagatcat
     4381 cacgcagcgc aacgtcgtga gcgacccggc cgaggcgaaa aaggtcctgg aggacacctc
     4441 cgagccctcc ggcctcagcg tgaagaggtc gggcttcgag gtcttcgtcc ccaggggcgg
     4501 cctccttgag cccgagacgg tctccctcgt ccccggcatc ctcgaggagg acgtgaagac
     4561 cgtcatactg gggctgatgg agaccatgag gtccgagaac accataacgg aacaggtgcg
     4621 ggtcctccac gtcttccgca acggcgacct gctttacctc gacctgaacg atcccttcgt
     4681 gaaggttgtc cagaaactga cggccgaaaa ggcgaccctg atcatgacgt cgctggttcg
     4741 gaccatcgtc gccaacttct cccccgtcac gagggtccgc atactgataa acggcaagga
     4801 cccggaactc aagacccccg tggacctcac cacgccgtgg cagctgaaga cctcatgacg
     4861 gggccggcgg gtgagatgcc ggggaagagg gccgacgggc gggaccccgg cgagcacagg
     4921 cccgtgaaca tgaccagggg gtacagccgt ttcgccgagg gctcggccct ggtggagttc
     4981 ggcgataccc gggttctctg cacagcctcc gtggaggaga gagtgcccct cttcatgagg
     5041 ggcgggggga agggctgggt gacggccgag tactcgatgc tgcccaggtc caccgagacc
     5101 cggacccccc gtgacagcgt ccgcgggggc gtcagcggca ggagccacga gatccagagg
     5161 ctcatcggcc gctccctcag ggccggcacc ttcctggaac tgctgggcga gcgcaccgtc
     5221 tgggtcgact gcgatgtcct ccaggccgac ggggggacca ggaccgccgc catcaccggc
     5281 ggtttcgtcg ccctcgccga cgccctgcgg tggctcttcg ccaggggggt catcgatacc
     5341 atcccgctga agtgcttcgt ggcggccatc agcgtcggga aggtcgacgg ttccctcgtc
     5401 acggatctct cctatggtga ggacagccgc gccgaggtgg acttcaacat agtcatgaac
     5461 gagttcgggg actttgtcga ggtgcagggc accgccgagg gtgaccccta ctcccggtcg
     5521 gaactgctca acatgatcgg cctggccgaa aggggcgtag cctccatgat ctcggtccag
     5581 aagagcgcgc tttcgctctc gggagaggaa gagagggccg tcgcccttgc cagggatcgt
     5641 attcgccagc ggcaacaggg ataagtacct cgagatgagg tccctcctcg cacccatggg
     5701 ggtggaactc ctcttcggcc cggacctggc gggctccccc ggcctcccgg cagtcgagga
     5761 ggacagggac acttacgccg gcaatgccat cgtgaaggcc ctgacgtggg ccatggccct
     5821 ggggcgctcc gccctggccg atgacagcgg gctcgaggtg aaggccctgg gctggggacc
     5881 gggcgtgatg tcggcccggg tcgcccccga tgacagggca agggtcgaat ggctcctcga
     5941 aaggatgagg ggggccgtcg aaagggaggc ccgcttcgtc gccgccctgg ccctttacga
     6001 gatcgatcgg ggacggtggt tcctggccga gggcttctgc tacggccgta tcgcgccggc
     6061 tccgtcgggg agatacggct tcggctacga tcccctgttc atcccccgcg gttacgacga
     6121 gaccctggcg gtgctggggc cggaagtgaa gtcccgcctt tcccaccgtt ccgtggcggc
     6181 cggggcgct
//
LOCUS       DAOSRG010000051         6104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11843_length_6104_cov_11.145313, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000051 DAOSRG010000000
VERSION     DAOSRG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11843_length_6104_cov_11.145313"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1483)
                     /locus_tag="PLM30_03075"
     CDS             complement(14..1483)
                     /locus_tag="PLM30_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HOR54186.1"
                     /translation="MIDLTINDAQLERTVGRARERNIVVPTFEQMKDPGKIPMKVREK
                     LKTVGLWDVDPANLFRVTWKNEPVPSGGLFGGVNLLELPGEITGVDARVVALVGKWFP
                     TGSHKVGPTLGCLVPRLVTGQFDPTAQKAVWPSTGNYCRGGAYNSQLLACDSIAILPE
                     GMSRERFEWLKTVAGEVIATPGSESNVKEIFDKTWELRRTRDDVVVFNQFEEFGNYLW
                     HHEVTGSALEEAFSIVAKPGDRFFGVTFTSGSAGTLAAGDRMKKIFPASKIAVGEAQQ
                     CPTLLMNGFGAHRIEGIGDKHVPWIHNVRNTDMVIDVDDADCMALIRLFNEPAGRGYL
                     ESRKLPRELIDQLPLMGISSAANVIMAIKMAKYYELTAKDIIFTVFTDSMELYGSRLR
                     EMEESFGVYGPVDAAVDHHRSILGLGTDWMKELSYYDRRGIHNLKYYTWIEQQGRDLE
                     ELNAQWYQADEYWGGIPPLAGRIDEKIRDFNRRTGLLEP"
     gene            1854..3062
                     /locus_tag="PLM30_03080"
     CDS             1854..3062
                     /locus_tag="PLM30_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOR54187.1"
                     /translation="MKKFVVILLGILVLAAMTSAAFAAAAFHIGICTGTVSQSEDDLR
                     GAEAMIAKYGDVSDGGMIKHLTYPDNFMQEMETTISQIASLADDPLMKVVIVNQAIPG
                     TTEGFRRVREKRDDILLFAGEAHEDPGVIESIADLTVNADNIARGYLIIAAAKKLGAT
                     DFVHISFPRHMSYELLSRRRNIMEEACKDLGIKFHFETAPDPTSDVGVAGAQQFVLEK
                     VPAWLEKYGDKTAFFCTNDAHTEPLLKRIAEGGGFFIEADLPSPLMGYPGALGVELAD
                     VKGDFPAILKKVEQAVIDHGGSGRMGTWAYSYGYTNSVALAEFGHKMVSAGVDAKNFA
                     RNFKPQDLFDVYSNATPGAEWNGNYYTDIQTGVVKKNHVLLYQDTYIFGKGYLDMTSV
                     EVPEKYLTFK"
     gene            3213..4817
                     /locus_tag="PLM30_03085"
     CDS             3213..4817
                     /locus_tag="PLM30_03085"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOR54188.1"
                     /translation="MALEPLLNVRGISKSFNHNKVLKNVSFSLEKGKILGLVGENGAG
                     KSTLMNILFGMTVIHETGGYEGTISIEGKKVRFASPFDALEAGIGMVHQEFSLIPGFT
                     VTENIVLNRESTKYNFLVEAYGDRMKTLDRDDMDRRSREAIDKLKVGLDPAIAVSELP
                     VGYKQFTEIAREIDKENTKILVLDEPTATLTESEAEILLDTMKRLAALGISIIFISHR
                     LQEIMDVCDKIIVLRDGEVVDNTTPDETDIMSIATSMVGRDITTAFAGKGDREGNLGE
                     VILRVEHLWVDMPGETVRDVSFQVREREIFGIGGLAGQGKLGITNGIMGMFPAGGSVF
                     FREGPIRLNDPTSPLEAGISAVYEDRRGVGLLMEEPISWNIIFTSLQMQERFLKPILG
                     GLIRIRDESEIARSARDYIDMLNIKCLSEDQRVQDLSGGNQQKVCLAKAFETKPRLLF
                     VSEPTRGIDVGAKRMVLEALRKYNDEFGTTIVMVSSELEELRSICDRIAIIDSGRIAG
                     ILPASAPSTDFGVLMLGIDGREKKVV"
     gene            4820..5860
                     /locus_tag="PLM30_03090"
     CDS             4820..5860
                     /locus_tag="PLM30_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54189.1"
                     /translation="MAGIKKFIENAGWPRIIIGLFLFSLFIMAPFVRVRIDASVSDTL
                     VRFGMNGVMVLAMVPMIVSGCGLNFGLPLGIIAGLLGAVTSIEIGIPGIPGVLIAMTI
                     SLPIAVVLGWGYGLLLNRVKGSEMMIATYVGFSSVAFMCIMWLVLPYKSSNMVWGYAG
                     KGLRTTIAVEGFWHQAISDIGSFSIGQFFYVPTGMFLFFALLCWFMWVFMRTRTGTAM
                     TTVGSNPEYARASGVNIDRMRTLSVILSTVLGAIGIIVYEQSFGFVQLYMGPFYMAFP
                     AVSAILIGGASVHRASILNVIIGTLLFQGILTMTPSVINSMIQTDMSEVIRIIVSNGM
                     ILYALTRTVKVK"
     gene            5860..>6104
                     /locus_tag="PLM30_03095"
     CDS             5860..>6104
                     /locus_tag="PLM30_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54190.1"
                     /translation="MNKKVDLLKHYLIQNAVPILFIVVSAFAIPISQFSGTYLVQEML
                     VRLSRNSFLVLSLLIPIMAGMGLNFGMVLGAMAGQIGL"
BASE COUNT         1311 a         1764 c         1702 g         1327 t
ORIGIN      
        1 tattcaaagg tcctcaaggt tccagcagcc cggtccttcg gttgaagtcc cttattttct
       61 catcgatccg gcccgccagg ggtggtatgc ccccccaata ctcatccgcc tggtaccatt
      121 gggcgttcag ttcttcgaga tccctgccct gctgctcgat ccaggtgtag tacttgaggt
      181 tgtggatacc cctcctgtcg taataagaca actctttcat ccagtcggtt cccaggccga
      241 ggatgctcct gtggtgatcg acggcggcat caacggggcc gtaaaccccg aaggactctt
      301 ccatctcccg taaccttgac ccgtagagct ccatcgagtc ggtgaaaacg gtgaagatga
      361 tgtccttcgc ggtcagttcg tagtatttcg ccatcttgat ggccattatc acgttggctg
      421 cgctggagat gcccatgagc ggcagttggt cgatcaattc ccggggcagc ttccgcgact
      481 ccagataccc ccgccccgcc ggctcgttga aaaggcggat cagggccatg cagtcggcgt
      541 cgtcgacatc tatcaccatg tccgtgttcc ttacgttgtg tatccaggga acgtgcttgt
      601 cgccgatccc ctcgatccgg tgggctccga aaccgttcat cagcagggtg ggacactgtt
      661 gggcctcacc gacggcgatc ttcgaggcgg ggaagatctt tttcatgcga tctccagccg
      721 ccagggtgcc cgcggaaccc gacgtgaagg tcaccccgaa aaagcggtcc ccgggtttcg
      781 cgacgatgga gaaggcctct tccagggcgg aaccggtcac ttcatggtgc cagagataat
      841 tgccgaactc ctcgaactgg ttgaagacca ccacgtcgtc cctggtcctc ctgagctccc
      901 aggtcttgtc aaagatctcc ttgacgttgc tctcactgcc gggggtcgcg atcacttctc
      961 ccgccaccgt cttgagccat tcgaacctct cgcggctcat cccctcaggg aggatcgcta
     1021 tcgagtcaca ggcgaggagt tgagaattgt aggcgccgcc gcggcaataa ttccccgtgg
     1081 agggccatac cgccttctgg gccgtcgggt cgaactggcc cgtcacgagc cttgggacaa
     1141 gacagcccaa agtgggaccg accttgtggg accccgtggg gaaccacttc cccacgaggg
     1201 ccacgacacg ggcgtcaact ccggttatct cccctggcaa ttccagcaag tttaccccgc
     1261 cgaagaggcc gccgctgggg acgggctcgt tcttccaggt aacgcggaaa aggttcgcgg
     1321 ggtcgacatc ccagaggccg accgttttta acttctccct gaccttcatg gggatctttc
     1381 cggggtcctt catctgttca aaggtcggga ccacaatatt cctctccctg gcccttccca
     1441 cggtcctttc aagctgggcg tcgttaatgg tgagatcgat cattactcca tccccctccc
     1501 ataggtttca tcatcgtcca tctcgttcac cggtgctttg ggctttcttt tctatcattt
     1561 ccccaatatc tccgcgggtc aaggccgtgc ggagcgtgac aagggttctt tgatcgcccc
     1621 ggctacggtc gacctcggat tcggggacgg gtgaaggtcg gtcatggaag agaccttgaa
     1681 cagacgggtt ctcgttaggc cgatgtatga tccgacatac cgttgtttta acgacaattt
     1741 ccttcgcggt tccggttgtg aacaccctcg aaacccaatt ccgaagttga caaaaaccct
     1801 cattggagtg atatttaagt gggtttaact aatctcaata agggggaatg gaagtgaaga
     1861 agttcgttgt cattctgctc ggtatcctcg tgttagcggc aatgacctcg gccgctttcg
     1921 cggcggccgc atttcacatc ggcatctgca cgggtaccgt ttcgcaatcg gaggacgacc
     1981 tcaggggcgc tgaagccatg atagccaagt acggcgacgt ctcggacggc ggaatgatca
     2041 agcatctcac ttaccccgac aacttcatgc aggagatgga gactaccatt tcccagatcg
     2101 cgtctctcgc tgatgacccc ctcatgaagg tcgtcatagt gaaccaggcc attcccggca
     2161 cgaccgaggg cttccgcagg gtcagggaaa agagggacga catcctgctc ttcgcgggtg
     2221 aggcccatga agacccgggt gtgatcgaga gcatcgccga cctcaccgtc aacgccgata
     2281 atatcgcccg cggatacctc atcatagccg ctgccaagaa gctaggcgcg acggacttcg
     2341 tccacatttc cttcccgcgc cacatgagct acgaactgct ctcccgtcgc cgcaacatca
     2401 tggaagaggc ctgtaaggac ctgggaataa aattccactt cgagacggct cccgacccga
     2461 ctagcgatgt cggtgtcgcc ggggcgcagc agtttgtcct cgaaaaggtc ccggcttggc
     2521 ttgagaaata tggcgacaag accgccttct tctgcacgaa tgacgcccat accgaacccc
     2581 tgctgaagag gatcgccgag ggcggcggct tcttcatcga agcggacctt ccctctcccc
     2641 tcatgggcta tcccggtgcc ctgggcgttg aactggccga tgtgaagggc gatttccccg
     2701 ccatcctcaa gaaggtcgaa caggccgtca tcgaccatgg cggatcgggc cgcatgggta
     2761 cctgggcgta ctcctacggc tacaccaact cggtcgccct ggccgaattc ggccacaaga
     2821 tggtgtcagc cggcgtcgac gccaaaaact tcgcccgcaa tttcaaaccc caggacctct
     2881 tcgatgtcta ctccaatgcc accccgggag ccgagtggaa tgggaactac tacaccgaca
     2941 tccagacggg ggttgttaaa aagaaccacg tcctccttta ccaggatact tacattttcg
     3001 gcaagggtta cctggatatg accagcgtcg aggtgccgga gaaataccta accttcaaat
     3061 aagatccctt tgcttagggg gagggggatt ccccctcccc tttttttgcc tggtcccctt
     3121 ctttctccgg tggctacttc tttctcaagc cgatggacta tggttactat atcgctcatg
     3181 aaaacgcact acccgctaga gaggtgaaat gcatggcctt agaacccctt ttgaatgtca
     3241 ggggaatcag caagtccttc aaccacaaca aagtcttgaa aaatgtatcc ttcagccttg
     3301 aaaagggaaa gatactgggg ttggtgggag agaacggcgc aggaaaatcg accttgatga
     3361 atatcctctt cggcatgacg gtgatccacg agaccggagg gtacgagggc accatctcga
     3421 tagaagggaa aaaggtgagg ttcgccagcc ccttcgacgc cctggaagcc ggaataggca
     3481 tggtgcacca ggagttctcg ctcatacccg gtttcacggt gaccgagaat atcgtcctca
     3541 acagggaatc gacaaaatac aacttcctcg tcgaggctta cggcgaccgc atgaaaaccc
     3601 tcgacaggga tgacatggac cgcaggagcc gtgaagcgat cgacaagttg aaggtaggcc
     3661 tggacccggc cattgccgtt tcagaactcc ctgtgggtta caagcaattt accgagatag
     3721 cccgcgagat cgacaaggag aacaccaaga tactcgtcct cgacgagccc acggcgaccc
     3781 ttaccgaatc ggaggcggag atcctcctcg atacgatgaa gcgcctcgcc gccctcggta
     3841 tttccatcat tttcatctcc cacaggctcc aggagattat ggatgtctgc gacaagatca
     3901 tcgtcctccg ggatggtgag gtggtcgaca acaccacccc ggacgagacg gatatcatgt
     3961 ccatagccac atcgatggtc gggagggata tcaccaccgc ctttgcagga aagggcgaca
     4021 gggaaggcaa cctcggggag gtcatcctcc gggtggagca cctctgggtg gacatgcccg
     4081 gggaaacggt gagggatgtc tctttccagg tcagggagcg ggagatcttc gggatcggag
     4141 gcctcgccgg ccagggaaaa ctagggataa cgaacggaat aatggggatg ttccccgcgg
     4201 ggggctccgt ctttttccgg gagggaccga tccggctcaa cgaccccacg agtccgctcg
     4261 aggcggggat atccgccgtt tatgaagacc ggcggggagt gggccttttg atggaagaac
     4321 ccatttcatg gaatattatc ttcacgtcgc tccagatgca ggaacggttc ctcaagccga
     4381 tcctcggagg cctcataagg atcagggacg aatcggaaat agccaggagc gccagggatt
     4441 acatcgatat gctcaatata aaatgcctca gtgaggatca acgcgtccag gacctttccg
     4501 gcggcaatca gcagaaggta tgccttgcca aggctttcga gacaaaacca cgactcctgt
     4561 tcgtgtcgga accgacgagg ggtatagatg taggcgccaa aagaatggtc ctggaagccc
     4621 tgagaaaata taatgacgaa ttcggaacga cgatcgtgat ggtctcttcc gaactggaag
     4681 aactgcgctc catctgtgac cggatagcga taatcgattc agggcgcata gcgggcatac
     4741 tcccggcttc ggccccctcg acggatttcg gcgtcctcat gcttgggata gacggaaggg
     4801 aaaagaaggt ggtctgacaa tggccgggat aaagaagttc atcgaaaacg cgggctggcc
     4861 gaggataatc ataggcctct tcctgttctc ccttttcatc atggccccct tcgtaagggt
     4921 ccggatcgac gcgtcggtga gcgacaccct cgtcaggttc ggcatgaacg gcgtgatggt
     4981 actggcgatg gtcccgatga tcgtatcagg gtgcgggctg aacttcggcc ttcccctcgg
     5041 cataatcgcc ggcctgctcg gggcggtcac cagtatagag atagggatcc ccggaatacc
     5101 gggggtcctg atcgccatga cgatatcctt gccaatagcc gtcgtgctgg ggtggggtta
     5161 cggcctcctc ctcaacaggg taaagggcag cgagatgatg atcgccacct acgtgggctt
     5221 ctcctccgtg gccttcatgt gcatcatgtg gcttgtgctc ccctacaaga gctccaacat
     5281 ggtctggggc tacgccggca agggcctccg aacgacgatc gccgtggagg ggttctggca
     5341 ccaggccatc agcgatatcg ggtccttcag catcggacag ttcttttacg ttcccacggg
     5401 gatgttcctc ttcttcgccc ttctctgctg gttcatgtgg gttttcatga ggaccaggac
     5461 cggcacggcg atgacaaccg tggggtcaaa tcccgaatac gccagggctt ccggggtcaa
     5521 catcgacagg atgaggacct tgtcggtgat attatcgacc gtcctgggag cgatagggat
     5581 cattgtctac gagcagagct tcggtttcgt tcagctttac atgggtcctt tctacatggc
     5641 cttcccggcg gtgtcggcga tactcatcgg cggggcctcg gtccacaggg ccagtatcct
     5701 taacgtgata atagggacat tacttttcca gggtatcctg acgatgaccc cttcggtgat
     5761 aaacagcatg atccagacgg acatgtcgga agtcatcagg ataatcgtat ctaacgggat
     5821 gatcctctat gccctcacac gaaccgtgaa ggtgaaataa tgaacaaaaa agtcgacctc
     5881 ctcaagcatt acctcatcca gaatgccgta cccatcctgt tcatcgtcgt gagcgccttc
     5941 gcgatcccga tctcccaatt ttccgggacc taccttgtcc aggaaatgct ggtacgcctt
     6001 tccaggaact ccttcctggt gctctcgcta ctcatcccta tcatggcggg tatgggcctg
     6061 aacttcggta tggtcctcgg cgcgatggca ggccagatag gcct
//
LOCUS       DAOSRG010000052         6064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_11932_length_6064_cov_8.680479, whole genome shotgun sequence.
ACCESSION   DAOSRG010000052 DAOSRG010000000
VERSION     DAOSRG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11932_length_6064_cov_8.680479"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="mnmE"
                     /locus_tag="PLM30_03100"
     CDS             complement(<1..1340)
                     /gene="mnmE"
                     /locus_tag="PLM30_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOR54191.1"
                     /translation="MRKDIIAAISTAWGESGIAIVRLSGSGSRDLVDSIFKGGGGLGP
                     SPPRFMKHGFILDADGEPVDEVLAVWFKGPYSYTGEEAAEIHCHGGTVAARKCLELCL
                     RGGARMARPGEFTRRAFLNGRIDLTEAESVLGVIRARSDGALKAAAKCLQGRLSDRLR
                     SILEELTDLSAAAEASMDHPDEDIPEVSTQIYLQRLSEVARRMRDLLSAARSGRFLRE
                     GIGVAISGRPNVGKSSLMNAFLEESRSIVTSMPGTTRDVIEEVISHKGVPLRLTDTAG
                     IRDPRDLAEEEGVKRSLEAIKEADIRVWVIDGSEPITADDRRIAASLQDSCCVIAVNK
                     SDLPLSVDPGEVKGLLPGREVRVVSAVTGQGLDELKDLIVLSVSGSTTVDEDVNTTER
                     QVEEIREALALLEEAEGAFASGVGLDAALDLLAGSRECLSRILGLSTDEDLLDR"
     gene            complement(1364..2578)
                     /locus_tag="PLM30_03105"
     CDS             complement(1364..2578)
                     /locus_tag="PLM30_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOR54192.1"
                     /translation="MDKFWGSNFSNKALKVRPSPIREMLSVVNQPGMISFAGGMPAPD
                     VFPVKEFHEGTEVFLRDGPSLLQYGTTEGFTPLKEYLSEWTAPRMGRKAALDEMLLTS
                     GSQQVLDLMGWAMIDPGDYVITEDPSYLAALTAFHNHGSRFIGIPTDAEGMVVDMLPE
                     KIEKARSEGKKVKFIYTIVNFQNPAGCTLSLERRKKLVEISNKYGVPIFEDDPYGYVR
                     FDGDHLPSIFSLDQEGGVIYAGSFSKILAPGTRIGWCTGSRDIIRKLTVFKQSTDLCS
                     SPISQALIYEYCKKGYLDAHLPKIVADYRAKRDAMEDSFKKYLPLDEVSWVKPEGGFF
                     YWIHMPHIDAEDLFKKAIEKKVAFVIGAPFFANGGGEHNARINYTYSKPDTIEEGVKR
                     LGEAMREMLGKA"
     gene            complement(2609..3796)
                     /locus_tag="PLM30_03110"
     CDS             complement(2609..3796)
                     /locus_tag="PLM30_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOR54193.1"
                     /translation="MKPSPLLELLDLGKKPGVISFAVGMPDPDIFPIEQLQKAASVIS
                     REGKDILQYGAAGGYPPLKGFLAEWTAPRMGRKLSTSELLITAGSAQVIDLLCWALLD
                     RGDWVICEEPTFQGATGTMFNHGARFLTVPCDSEGMKVELLPERIERIRAAGEPIKFI
                     YTIVNFHNPLGCDLSLQRRARLLEIARTYGILILEDDPYGWVRFEGEDIPSLFSMDDS
                     GLVVFSSTFSKILAPGTRVAWCAGREEIIRKMTVLKENVDTCTSVLAQAIVWEYCRLG
                     YLDAFLPRIIDHYRKKRDGMETALKKHLPPDRVSWQKPGGGFFYWLTVPGIPAVQLLE
                     KALEKKVAFVPGNAFYPTGSGGLNNFRMCFTFASTEMTDLGVRYIGEAIREFEQEPGH
                     KKS"
     gene            complement(3926..4561)
                     /locus_tag="PLM30_03115"
     CDS             complement(3926..4561)
                     /locus_tag="PLM30_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOR54194.1"
                     /translation="MIRKITGSILVLFLLATYPMTGGAAIDPGSVDRVWRDLTSTASL
                     AEAGPVSIEGKDEPNAWVSFTMSKYSVHATTGLLATLRSTDELAGVLAHEIGHIKLGH
                     YNETVKRNLLWGLLYMALGERKIGGFDVVGTGVVLAEAGFSREQEIAADDYGVALAAK
                     AGYDPWGLYNALQGMAKSGFKTTPSGFNSHPPTERRLAHIRETTERISKGN"
     gene            4722..5933
                     /locus_tag="PLM30_03120"
     CDS             4722..5933
                     /locus_tag="PLM30_03120"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOR54195.1"
                     /translation="MLDRLEILKELSVENRTKIVLLVMDGLGGLPGTGGQTELEAAFT
                     PNLDALASRAELGLLEMVDTGVTPGSGPGHLGLFGYDPLKFQIGRGILEALGTGAHVQ
                     AGELCARGNLCTWGEKDLVLDRRAGRIDTGSSAGIIGKLAERIKEIDGFGVRFYPGVE
                     HRFAVVFSGKGLSDKVTDADPQVEGRPMIWSEPLDPSAERTSRLVNDLIRKVREVLSD
                     EPKANGCLLRGISGAPDIPPMPDLYRFRPAAIATYPMYKGLARIVGMEVLPAGKTVEG
                     LFQALGTNWAAFDFFYVHVKYADSRGEDGDFDAKREVIEKVDSLVPEVLELEPDVLVV
                     TGDHSTPSVMASHSWHPSPFLLASPLVRSDGSPSFGERSCARGSLGIMPAYKLMGLML
                     AHARRLSKYGA"
BASE COUNT         1139 a         1816 c         1846 g         1263 t
ORIGIN      
        1 atcctgtcca ggaggtcttc atcggtcgat aggcccagta tcctcgagag gcattcccgt
       61 gaaccggcca gcaagtccaa ggccgcgtca aggccaacgc cggaggcgaa cgctccttcg
      121 gcttcttcca gcagtgccag ggcttccctt atttcctcca cctgccgttc ggtggtgttg
      181 acgtcctcgt cgacggtggt gctcccggag acggaaagga cgatcaggtc cttgagctca
      241 tccagcccct ggccggtgac ggccgagacg accctgacct cgcggcccgg aaggagcccc
      301 ttgacctccc ccgggtcgac cgacaggggc aggtccgact tgttgacggc gatgacacag
      361 caactatcct ggagtgaagc tgctatcctc ctgtcgtcgg cggtgatggg ttcgctgccg
      421 tcgatcaccc aaaccctgat atcggcttcc ttaatggcct ccagggaacg cttcacccct
      481 tcctcctcgg ccaggtccct ggggtccctt atcccggccg tgtccgtcag cctgaggggc
      541 acccccttgt gggagatcac ctcttcgatc acgtccctgg tcgtcccggg catggaggtg
      601 acgatcgaac gggactcctc gaggaaggcg ttcatgaggg aggatttccc cacgttggga
      661 cggccggaga tcgcgacccc gatcccctcc ctcaagaaac ggccggatcg tgccgccgag
      721 agtagatccc tcatcctccg agccacctct gagagtctct gaagatagat ctgggtgctt
      781 acctcgggta tgtcctcatc gggatggtcc atggaggcct cggccgccgc ggagaggtcg
      841 gtcagttctt cgaggatgct cctgagccgg tcggaaagcc gaccctggag gcacttggcc
      901 gcggctttca gggccccgtc ggagcgggct cttatcacac ctagcactga ttcggcctcg
      961 gtcaggtcga ttctgccgtt caggaaggct ctcctggtga actctcccgg gcgggccatc
     1021 ctggcgcccc cgcgaaggca gagttcaaga catttcctcg ctgccaccgt ccctccgtgg
     1081 cagtgtattt cggccgcttc ctcaccggtg tagctgtagg gtcccttgaa ccagaccgcc
     1141 aacacttcgt cgactggctc gccgtcggcg tcgagaatga agccatgctt catgaagcgg
     1201 ggcggggagg ggcccagccc ccccccgccc ttgaagatcg aatcgacgag gtccctggag
     1261 ccactgcctg aaaggcgcac gatggctatg ccggactcgc cccaggctgt tgatatagcg
     1321 gcgatgatgt ccttccgcaa ggaaccgggt ccctcgccca tggtcatgct ttcccgagca
     1381 tttccctcat ggcttccccg aggcgtttga ccccctcttc aattgtatcc ggcttggagt
     1441 aggtgtagtt gatcctcgcg ttgtgctccc cgccgccgtt ggcgaagaag ggagccccga
     1501 tgacgaaggc gaccttcttc tcgatggcct tcttgaaaag gtcctcggca tcgatgtggg
     1561 gcatgtggat ccagtagaag aaaccacctt ccggtttgac ccaggagacc tcatcgaggg
     1621 gcaggtactt tttgaaactg tcctccatgg cgtctctttt ggccctgtaa tcggcgacga
     1681 tcttcggaag gtgagcgtcc aggtagcctt tcttgcagta ctcgtagatc agagcctggg
     1741 agatggggct cgagcagagg tccgtggact gcttgaagac ggtcagtttc cttattatgt
     1801 cacggctgcc ggtgcaccac ccgatcctgg tccccggggc aaggatcttc gagaaggagc
     1861 cggcgtagat gacgcccccc tcctggtcaa gggagaaaat ggaaggaagg tggtcaccgt
     1921 caaaacggac gtaaccgtaa gggtcatcct caaagatggg gaccccgtat ttgttggaga
     1981 tctccaccag tttttttctt ctctcaaggg acagggtgca tcccgcgggg ttctggaagt
     2041 tgacgatcgt ataaatgaac ttgaccttct tgccctcgga gcgggccttc tcgatcttct
     2101 cggggagcat atccacgacc atcccctcgg cgtcggtggg gatgccgatg aaacgggagc
     2161 cgtggttgtg gaacgccgtg agggccgcca ggtacgaagg gtcctcggtg atgacgtagt
     2221 caccggggtc gatcatggcc caacccatca ggtcgagcac ctgctgcgaa cccgaggtga
     2281 ggagcatttc gtccagggcg gcctttctgc ccatcctggg cgccgtccac tccgagaggt
     2341 actctttcaa gggagtgaaa ccctcggtgg tgccgtactg gaggagactc gggccgtccc
     2401 gcaggaagac ctcggtcccc tcgtgaaact ccttcaccgg gaagacatcg ggcgcgggca
     2461 tcccgcctgc gaaggatatc attcccggtt ggttcactac gctcaacatc tcccgtatcg
     2521 gcgagggacg aaccttaagc gccttgttgc tgaaattcga tccccagaac ttgtccaatt
     2581 cgatctcctc caatatggat cagatgtctc agctcttctt atggccaggc tcttgctcga
     2641 actcgcggat ggcttcaccg atgtatcgga caccgaggtc cgtcatttcg gtggaggcga
     2701 aggtgaagca catcctgaaa ttgttgagtc cccccgaccc ggtggggtaa aaggcgttcc
     2761 ccgggacgaa ggcgaccttt ttctccagag ctttttccag gagttgcacg gcaggtatcc
     2821 ccgggaccgt caaccagtaa aagaagccgc ctccgggttt ttgccaggac accctgtccg
     2881 ggggcaggtg ctttttcagg gcggtttcca tgccgtccct ctttttcctg taatgatcga
     2941 ttatgcgggg gagaaaggcg tcgaggtagc ccagccggca gtattcccat actatggcct
     3001 gggccaggac actggtgcag gtatcgacat tttccttaag caccgtcatt ttcctgatga
     3061 tctcctccct ccccgcgcac catgcgacgc gggtgccggg ggccaggatt ttggagaagg
     3121 tcgatgagaa taccaccagg ccgctgtcgt ccatggagaa cagcgagggg atatcctcac
     3181 cctcgaagcg gacccagccg taggggtcat cctcaaggat caggatcccg taggtcctgg
     3241 cgatctctag caaccttgcc cttctctgga gtgagaggtc gcagcccagc gggttgtgga
     3301 agttgacgat ggtatagatg aacttgatgg gttctccggc ggcccggatc ctttcgatcc
     3361 gctccgggag cagttccacc ttcatcccct cactgtcgca agggacagtc aggaacctgg
     3421 cgccgtggtt gaacatggtg cccgtcgcgc cctggaaggt gggttcctcg cagatcaccc
     3481 agtcgcccct gtccagcagg gcccagcaaa gcaggtcaat cacctgggcg gagccggcgg
     3541 tgatcagcag ttcgctggtt gacagttttc gtcccatcct cggggcggtc cattcggcca
     3601 ggaaaccctt gagcggcggg taaccccccg ccgcgccgta ctgcagaata tccttgccct
     3661 ctcttgagat gacggaagcg gctttttgca actgctcaat ggggaagatg tcggggtcgg
     3721 gcatacccac cgcgaaggag atcacccccg gttttttgcc gaggtccagc agctccagga
     3781 gaggggaggg tttgattttc tttgccgctg tgctgaaaag gccgtcgagg aaaacctccg
     3841 ttttattcgc acctccgtcc aggcgagggt gacgaccccc agcatgggga gtccgtcttt
     3901 attctatatc attatcctag gatcatcagt ttcccttgct tatcctttcc gtggtctccc
     3961 tgatgtgggc cagacgccgc tcggtagggg ggtgtgaatt gaaaccgctc ggggtcgttt
     4021 tgaatcctga cttagccatg ccttggaggg cgttgtaaag gccccagggg tcgtaaccgg
     4081 ccttcgcagc cagtgccacc ccgtagtcgt cggcggcgat ctcctgttcc ctgctgaaac
     4141 ccgcttcggc gaggacgacc ccggtaccga ccacatcgaa gccgccgatc ttcctttccc
     4201 ccaaggccat gtagagcagg ccccaaagga gattcctttt tacggtttca ttgtagtggc
     4261 ccagcttgat gtgacctatc tcgtgggcca gtaccccggc cagttcgtcc gtggaacgga
     4321 gcgtagccag gagccccgtc gtggcgtgaa ccgaatattt gctcatcgtg aaggaaaccc
     4381 acgcgttggg ttcatccttg ccctcgatgc tcacggggcc cgcctccgcg agggacgccg
     4441 tggaggtcag gtcgcgccag actctgtcga cggagccggg gtcgatcgca gccccacccg
     4501 tcattgggta cgtcgccaat aaaaagagta ccaggatgga acctgtgatc ttcctgatca
     4561 aggagcccgc ctcccttcga tttgcctggc ggcattatgg gacatgaggg cggagaaaat
     4621 aaagggtcaa atggcttatt atattatcct ggcaaggcgg gaaaaaaggc aagagaagct
     4681 ccgcgcaggg cggcgcttct ataggggagg aggaatgcct tttgcttgac aggctggaga
     4741 tcctcaagga actttcggtc gaaaacagaa caaaaatagt acttctcgtg atggatggcc
     4801 tgggggggtt gccggggacc gggggtcaaa cggaactgga ggcggccttc acgcccaacc
     4861 tcgatgccct ggcttcccgt gcggagttgg gacttctcga aatggtcgat acgggggtca
     4921 ccccgggaag cgggccgggg cacctcggcc ttttcggcta cgatcctctg aagttccaga
     4981 tcggcagggg gatactggag gccctgggca cgggggccca tgtccaggcc ggggagctct
     5041 gtgccagggg gaacctgtgt acctggggtg agaaggacct cgtcctcgat cggagggccg
     5101 gtcggatcga caccggttcg agcgccggga tcatcgggaa actggccgaa aggataaaag
     5161 agatcgatgg gttcggtgta agattctacc ccggagtcga gcaccgtttc gccgtggtct
     5221 tctcgggaaa ggggttgagc gacaaggtga ccgacgcgga cccccaggtc gaaggccggc
     5281 ccatgatctg gagcgaaccc ctggaccctt ccgccgaaag gacgtcccgc ctggtcaacg
     5341 acctcatccg aaaggtccgg gaggtccttt ccgatgagcc gaaagccaac ggctgccttt
     5401 tgaggggcat atccggagcg ccggacatcc cccccatgcc tgacctctac cggttccgcc
     5461 ccgcagcgat agccacctac cccatgtaca aaggcctggc caggatagtg gggatggagg
     5521 tcttgccggc gggcaagacg gtggaaggcc tcttccaggc cttagggacg aactgggcgg
     5581 ctttcgattt cttctacgtt catgtaaaat acgccgacag ccgcggcgag gacggtgact
     5641 ttgacgcgaa acgtgaggtc atcgaaaaag tcgacagcct cgtgccggag gtgcttgagc
     5701 tggaacccga tgtcctcgtc gtgaccggcg accacagcac cccgagcgtc atggcttccc
     5761 actcatggca cccctcgccc tttctcctgg cgagccccct ggtgaggtcc gacgggtcgc
     5821 cctccttcgg cgagagaagt tgcgccagag gaagccttgg gatcatgccg gcctataagc
     5881 tcatggggct gatgctggcc catgcccgaa ggctttccaa atacggagct tgaccccttc
     5941 caggtgcttc cggtcacgaa ggtgggccga aaggagcgtc gtcactccag cggcggcgta
     6001 agtgaatatt tgaaacggag ggatctcctt gtccgacatg atgaactcaa ctacatccca
     6061 gctg
//
LOCUS       DAOSRG010000053         5973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_12151_length_5973_cov_8.830686, whole genome shotgun sequence.
ACCESSION   DAOSRG010000053 DAOSRG010000000
VERSION     DAOSRG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_5973_cov_8.830686"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLM30_03125"
     CDS             <1..674
                     /locus_tag="PLM30_03125"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOR54196.1"
                     /translation="AIRIFETGMVFFRRGREVEEVFKLGGCVCTGVDSRSPWGDQGTE
                     DFHSVAADIEAIFASRGVAVDLVPGTEPFGHAGKTARVLADGRDVGYLSALKPEIESE
                     MELPAPAFLFELDMGPLVHDGKATFGEVRKYPPVFRDISLVVKEGVYSSDVLALIREL
                     GGPLLEEVRLFDIYKGKGIPEGHRSLAYTLAYRDPERTLRDDEIDQVQGRLRDQLAQR
                     GIRLR"
     gene            690..1070
                     /locus_tag="PLM30_03130"
     CDS             690..1070
                     /locus_tag="PLM30_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54197.1"
                     /translation="MLDQIDSLESLIDKVSAAMSELKTEKDRMGEELETLRGVLDGKE
                     KEMDRLSEEREVTEKRLAALLEKLRASWEPAAEQDPPADQDPPEAEEEPDPLESEEAP
                     PEAPAGELFRFDKNPHQGDLADRF"
     gene            1091..1333
                     /locus_tag="PLM30_03135"
     CDS             1091..1333
                     /locus_tag="PLM30_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54198.1"
                     /translation="MQEDPRRVLVTLGKKSYPLLTRLDEGRFERVLQIAKESVVGLDP
                     SMEQDERLLLACFKLAFSIESAEIRMKELLGGSGSP"
     gene            1330..1896
                     /locus_tag="PLM30_03140"
     CDS             1330..1896
                     /locus_tag="PLM30_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOR54199.1"
                     /translation="MTLALGTDILFALLLVTFCVRGAAKGFSGEVISLLATIGGFLLA
                     WKVSAIASSFISPYLPLRPGATRAVVLVVFYVLFLVAGVYVGRMVKSFLRFTHLSGVD
                     RGMGIVAGAVKAFALLVIVYAALMALSPVLSPYWMNESVAMKMAEKSWPTIERTLSSI
                     RLLDPASLPWEPGPHDPGDGAGIAEPVI"
     gene            1950..4244
                     /locus_tag="PLM30_03145"
     CDS             1950..4244
                     /locus_tag="PLM30_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOR54200.1"
                     /translation="MSRRTRSEAGGSMLRGMLPASSIKAARQRQELLKSYARYVETRG
                     ELPWDERMKPLGPLLENAGETALLSGVELLPFRILIGLSKRIKESLREARNDIPGVER
                     LAEGIRDLDEEFEKLQVLSDDGELYDGASEKLAELRLSLRDLTSRARSRCAALSVEPS
                     LAPMLQDRVVHFRNGRFALLVRAEDSGRFDGIVLDRSSSGKGVYLEPKELVPLNNRIQ
                     VLRREESEEERRILSALTTMILTRRLPLLETESAVARIDVLYACDRFLVEPPWTLPEL
                     DDRSRFHFKELWNPLIGKECVPVSIHCGERFRQLIITGPNTGGKTVVLKSVAIGIFLA
                     LSGFPVPAAEGTQVGWIDFMAADIGDEQGIEQSLSTFSSHIRRIIAMMEKAGKDSLLL
                     LDELGAGTDPQEGAALGIAILEALRERGSLVLATTHHNPIKRYALGSRGVEAAGMEFD
                     PATLAPTFRLIMGLPGKSNALFIADRLGMPMNIIDSAREHLSEETRTGEKVMTELFDK
                     QVELEAAGAAMAEERKELATLRRELEEKIRGLDERQERILEAADKKASAVVDEAEKAA
                     LEMLNRLRGTAESSARREYGKARKEMQPLKDRLIDREGSRARKRMEREGEPRVGDVVR
                     LAGTEVRGVVESVRGDKATVVAGSMTIEVPLKDLAREKGKAPPQTVRPALVSHVPAPR
                     GVPPSIMVRGMTVDEALPEVEVYLDRAFRAGYGEVVIIHGRGEGILRRAVHSLCRSLP
                     YVGDFRLGHAGEGGYGVTVVSFRR"
     gene            4334..4410
                     /locus_tag="PLM30_03150"
     tRNA            4334..4410
                     /locus_tag="PLM30_03150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4368..4370,aa:Arg,seq:ccg)
     gene            4500..5306
                     /locus_tag="PLM30_03155"
     CDS             4500..5306
                     /locus_tag="PLM30_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOR54201.1"
                     /translation="MQIAVLIKQVPDSDDVRMDEVAGTMIREGAGAMINPLDLHALQE
                     ALTIRSSRGGKVVALSMGPSQAEAALREALALGADAVVFLSDPHFAASDTWATARTLA
                     AACERTGPYDLILAGEKATDGETGQVGPEVAVMLGLPFSTYVSRVEFDGCGSVEVSRT
                     VEDGIERQKLPLPCLLTVLRDINEPAMPTLSGKKAARRAAFTVQGMSFLGLSPDEAGL
                     AGSPTRVRKIFRPSIARSPRIFKAGDIEEGIAALFDELEGRGLIDERGSS"
     gene            5303..>5973
                     /locus_tag="PLM30_03160"
     CDS             5303..>5973
                     /locus_tag="PLM30_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR54202.1"
                     /translation="MRNPSASGDILVLGEVRSGRLHRATLELTSKARSLADVLGGEVA
                     TLLMYDRIDRDPRTIVHYGADRVICVRDERLRLFDQEVQSTILSFVIGSTGPGIVLAP
                     ATTSGRTIMPAVAAMVGTGLTADCTGLEIEAETGLLLQTRPAIGGNIMATITTPDDLP
                     QMATVRPRTFAVPEPDPDREGRVEELTLPDRCFESRVEPLGYEGSSGDDVNIQDRDVI
                     ISGGKG"
BASE COUNT         1201 a         1735 c         1996 g         1041 t
ORIGIN      
        1 gggccataag gatcttcgag acaggcatgg tctttttcag gaggggccgg gaggttgagg
       61 aggtcttcaa actggggggg tgcgtctgta ccggtgtcga cagccgttca ccctggggag
      121 accaggggac ggaagacttc cattccgtcg ccgcggatat cgaggcgata ttcgcgagcc
      181 ggggggttgc cgtggacctt gttcccggaa ccgagccctt cgggcacgcc gggaagacgg
      241 cacgggtgct ggccgacggg cgggatgtcg gctatctttc ggccctcaag ccggagatcg
      301 agtcggagat ggaacttcct gcgccggcct tcctttttga actggacatg ggacccctgg
      361 tccatgacgg gaaggcgacc ttcggagagg tccggaagta tccccccgtc ttcagggata
      421 tttccctggt ggtgaaggag ggggtctatt cctcggatgt cctcgccctt atccgtgaac
      481 tggggggccc gctcctggag gaggtgagac tcttcgacat ttacaagggg aaagggatcc
      541 cggagggcca tcgaagcctc gcctacaccc tggcctaccg tgaccccgaa aggacattga
      601 gggatgacga gatcgaccag gtccagggaa ggctcagaga tcaactggcc cagagaggga
      661 taagactaag ataaagggga gagaacgaaa tgcttgacca gatcgacagt ctggagagcc
      721 tcatcgacaa ggtatcggcg gccatgtccg aactgaagac cgaaaaggac cggatgggcg
      781 aggaattgga aaccctcagg ggcgtcctgg acgggaagga aaaggagatg gacaggctca
      841 gtgaagaaag ggaggtcacc gagaaaaggc tcgccgccct cctggagaag ttgagggcct
      901 cctgggagcc cgcggcagaa caggatcccc cggcagacca ggaccccccg gaagcggaag
      961 aagagcctga ccctcttgaa agtgaagagg ctccgccgga ggcacccgcg ggggaacttt
     1021 tcaggttcga taagaacccc caccagggcg accttgcgga tcgtttctga cccagggagg
     1081 agggccccag ttgcaagagg atccgcgcag agtccttgtc acgctgggca agaaaagtta
     1141 ccctctgctg acccgccttg atgagggcag gttcgagagg gtcctgcaga tagccaagga
     1201 gagcgtcgta gggcttgacc cgtcgatgga acaggacgag aggctcttgc tggcctgttt
     1261 caaactcgcc ttttccatcg agtccgccga gatcaggatg aaagagctgt tgggggggag
     1321 tggatctcct tgaccctcgc cctgggaacg gacattctct tcgcccttct cctggtgacc
     1381 ttctgcgtca ggggcgcggc aaagggtttt tccggggagg tcatttcgct cctagcgacc
     1441 ataggcggtt tcctgctggc ctggaaagtt tcggccatcg cctcgtcctt tatttctccc
     1501 tatttgcccc tgcgccccgg ggcgacccgg gccgttgtgc tcgtagtatt ctacgtcctt
     1561 tttcttgtgg cgggggtcta tgtcggcagg atggtcaaga gcttcctcag gttcacccat
     1621 ctctcgggcg tcgacagggg aatggggatc gtcgccgggg ccgtcaaggc ctttgcgttg
     1681 ctggtgatcg tctacgcggc cctcatggcc ctttcccccg tcctctcgcc ctactggatg
     1741 aacgaaagcg tggccatgaa aatggccgaa aagtcgtggc cgaccatcga aaggaccctt
     1801 tcctccatcc ggctcctcga ccccgcatcg ctcccgtggg aaccaggccc ccacgacccg
     1861 ggggatggcg cgggaatagc ggagcccgta atttgaaggt cgaacaaaga accgctgatt
     1921 tggcagagat cacctccgtc ctggaaagga tgtccaggag gacaaggtcg gaggccggcg
     1981 ggtccatgct ccggggtatg ctgccggcct cgtcgatcaa agccgcccgg cagcggcagg
     2041 aactcttgaa gagctacgcc cgttatgtcg aaacgagggg cgaactgccc tgggatgaaa
     2101 ggatgaagcc cctggggcct ctcctggaga acgccggcga gacggcgctc ctttcggggg
     2161 tggagctgct tcccttccgg atactgatag ggctgtccaa gaggatcaag gagagtctcc
     2221 gggaagcgcg caatgatata cccggggtcg aaaggctcgc cgaagggatc agggacctcg
     2281 acgaggaatt cgagaagctc caggtcctgt ccgatgacgg tgagctctac gacggcgcgt
     2341 cggagaaact ggccgagtta aggctgtccc tcagggacct cacgtccagg gccaggtccc
     2401 ggtgcgcggc gctctcggtg gagcccagcc tggccccgat gctccaggac agggtcgtgc
     2461 acttcaggaa cgggcgcttc gcactcctgg tcagggcgga ggattccggg cggttcgacg
     2521 ggatagtcct ggacaggtcc tcctccggca agggtgttta cctggagccg aaggaactcg
     2581 tcccgctgaa caacaggatc caggtcctcc ggcgcgaaga gagcgaggaa gagaggagga
     2641 tcctctccgc cctcaccacg atgatcctga cgaggagact tcccctcctc gagaccgagt
     2701 ccgccgtggc acggatcgac gtcctttacg cctgcgaccg ctttttggtc gaacctccct
     2761 ggaccctgcc cgaactggat gaccggtccc gcttccactt caaggagctc tggaaccccc
     2821 tgatcggcaa agagtgcgtg ccggtgagca tccactgcgg agagaggttc cgccagctca
     2881 tcattacagg tccaaacacc ggcggtaaga cggtggtgct gaagtcggtg gcgatcggca
     2941 tcttcctggc cctgtcgggt ttccccgtcc ccgcggccga ggggacccag gtgggctgga
     3001 tcgacttcat ggcggccgac atcggcgatg aacagggcat cgagcagagc ctctccacct
     3061 tcagttccca cattcgaagg atcatagcca tgatggaaaa agccggcaag gacagccttc
     3121 tcctgctgga tgaactcggg gccggaacgg acccccagga gggggccgca ctggggatcg
     3181 ccatccttga agccttgagg gaaaggggtt ccctggtcct cgcgacgacc caccataacc
     3241 ccataaagcg ctacgccctg gggagcaggg gggtcgaggc ggccggaatg gagttcgacc
     3301 cggccaccct ggcgccgacg ttcaggctga tcatggggct ccctggaaag agcaacgccc
     3361 ttttcatcgc cgacaggctg gggatgccga tgaatatcat cgactcggcc agggagcacc
     3421 tatccgaaga gacccgcaca ggggagaagg tcatgaccga actcttcgat aaacaggtcg
     3481 aactggaggc cgccggggcg gccatggccg aggagaggaa ggaactcgcc accctcaggc
     3541 gcgaactcga ggagaagatc cggggcctgg acgaaaggca ggagaggatt ctggaggccg
     3601 ccgacaaaaa ggccagcgcc gtcgtggacg aagcggaaaa ggcggccctg gaaatgctca
     3661 accgtctgcg agggacggcc gaatcatcgg cccgcaggga atacggaaag gcaaggaaag
     3721 agatgcagcc cttgaaggat aggttgatcg acagggaggg gtcccgggcg aggaaaagga
     3781 tggaaaggga aggcgaaccc cgggtcggcg acgtggtcag gctcgccggt accgaggtca
     3841 gaggggtcgt cgagtcggta aggggggaca aggcgacagt ggtggcgggt tccatgacga
     3901 tagaggtccc gctgaaggac ctcgccaggg aaaagggaaa ggctccgccc cagacggtga
     3961 ggccggccct ggtctcccat gtcccggctc caaggggggt ccccccttca ataatggtga
     4021 ggggcatgac cgtggatgaa gccctgcccg aggtggaggt ttaccttgac agagccttcc
     4081 gcgccgggta cggtgaagtg gtcatcatcc acgggcgggg agaagggata ctcaggaggg
     4141 cggtccactc actctgccgc agcctgccct acgtcggcga tttccggctt ggccacgccg
     4201 gggagggggg ctacggggtg acggtagtat ccttcagaag atgaggggcg ggatggtgga
     4261 cgatgaggcg ccgggaataa aggtcccctt gacaacccaa cggcgggggc gttaacctag
     4321 gcgccgcgga tgcgtgtccg tagctcagct ggatagagcg tcggcctccg gagccgaagg
     4381 tctcgggttc aaatcccgac gggcacgcca ttccccttcg atcgccgcaa ccggctcctc
     4441 cagggtgagg ggccggtttt tcatttaagg gaccctaaaa ttttaaagga tgtgacccag
     4501 tgcagatcgc agtactcata aaacaggtcc ccgattccga cgatgtccgt atggatgagg
     4561 tcgcagggac gatgatccgg gagggagcgg gggccatgat caaccccctg gacctccacg
     4621 ccctccagga ggctttgacc atccgttctt cccggggagg gaaagtggtc gccttgtcga
     4681 tgggaccgtc ccaggcagaa gccgcgctcc gggaggccct tgccctaggt gccgatgctg
     4741 tggtcttcct gagcgatccc cacttcgccg cgtcggacac ctgggccacg gcccggacgt
     4801 tggccgcggc ttgtgaaaga accgggccct atgacctcat cctcgccggt gaaaaggcga
     4861 ccgacggcga gacggggcag gtggggcccg aagttgctgt aatgctgggc ctccccttca
     4921 gcacctatgt cagccgtgtt gaattcgacg ggtgcggctc cgtggaggtc tcacgaaccg
     4981 tggaggatgg gatcgaaagg cagaagctcc ccctcccctg ccttcttacg gttctccgcg
     5041 acatcaatga acctgcgatg cccacccttt cggggaaaaa agcggcccgc agggccgcct
     5101 tcactgttca gggcatgagt ttccttggac tttcacccga cgaagcgggg ctagccgggt
     5161 ctcccacgag ggtgaggaag atcttccgcc catccatcgc caggagcccc aggatcttca
     5221 aggctggaga tattgaggag gggatagcgg cgttgtttga tgaactcgag ggccgcggcc
     5281 tcatcgatga gcgggggtcg tcatgagaaa cccttctgca tcgggggaca ttctcgtcct
     5341 cggtgaggtc aggtcggggc ggctccacag ggcgacgctg gagctcacgt caaaagcgag
     5401 gtccctcgcc gatgtcctgg gaggcgaagt agcgacgctg ctgatgtatg accggatcga
     5461 cagggacccc cgaacgatcg ttcattacgg ggcggatcgg gtgatatgcg tccgggacga
     5521 gcggctccgc cttttcgacc aagaggtcca gtccaccata ttatctttcg tcatcgggag
     5581 taccggtccc gggatagtgc tggccccggc cactacctca ggcaggacca tcatgcccgc
     5641 cgtcgccgcc atggtcggga caggactgac cgccgactgc accgggctgg aaattgaagc
     5701 cgagacaggg ctgctccttc aaacgcgacc tgccattggc ggcaacatca tggccaccat
     5761 caccaccccg gatgacctgc cccaaatggc gaccgtccgg ccgaggacct tcgccgtccc
     5821 cgaaccggac cctgacaggg aaggccgggt cgaggagttg accctcccgg accgctgttt
     5881 tgaaagcagg gtcgaacccc ttgggtatga gggttcctcc ggggacgatg tcaacatcca
     5941 ggaccgggat gtgatcatct ccggcggcaa ggg
//
LOCUS       DAOSRG010000054         5944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_12227_length_5944_cov_10.789947, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000054 DAOSRG010000000
VERSION     DAOSRG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12227_length_5944_cov_10.789947"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLM30_03165"
     CDS             <1..258
                     /locus_tag="PLM30_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOR54203.1"
                     /translation="DQLIVSGVESHICVLATVMDLLSQGYPVAVAADACSSRDPDHHR
                     MACEAMTAAGALVAPVETLAYQLIRRAGTEQFKAMLPFFKA"
     gene            296..1540
                     /locus_tag="PLM30_03170"
     CDS             296..1540
                     /locus_tag="PLM30_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOR54204.1"
                     /translation="MPIDRTRAIELHRKARGKIKIYPTINIHNEEEMALAYIPGSVPP
                     ALAIQQDEGMSFEYTGRGNRIAVVTDGSAVLGLGNVGPYAALPVIEGKCLLFKLFGDV
                     NAYPLCVGSQDTEDILHFCALLAPTLGGINIEDISSPKAFTIVRELRNRINIPVLCDD
                     QHGTAVIVLAGIYNALEVQGRKLEDMKIVINGAGSAGIATAELLLKTGAKDIVVLNSA
                     GILGEDNPRMNHTQEELAEITNPGKITGGLDKAMEGANIFVGLSKGGVLPPGNIRLMS
                     GDPVILAMALPEPEILPEEAIAAGAAIVATGGPGYQNAMPNSLSTPGIMRGLLDVRAT
                     VLNHTMLLAAARALADVVDRRRLGPGKIIPDIFCDETAPRVAEAVGQAAIAEGFATKE
                     VPKGEIYNNLWQNLYGEQITRF"
     gene            complement(1684..>2170)
                     /locus_tag="PLM30_03175"
     CDS             complement(1684..>2170)
                     /locus_tag="PLM30_03175"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOR54205.1"
                     /translation="MRPGTPVHATGLAAVALGQGVRFWAAGSIRQYRGETVGAATLVT
                     WGPYALARNPLYLGNALIGTGWCALTGKPMAFVIFLVVFITLYVLLVIPYEEGYLKER
                     FPGEFARYSERVGRFFPLRAPSPAKLKGPFDWGVLWKSERHSLWVTLIGTLLLLSRRW
                     W"
     assembly_gap    2171..2270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2271..3288)
                     /locus_tag="PLM30_03180"
     CDS             complement(<2271..3288)
                     /locus_tag="PLM30_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOR54206.1"
                     /translation="MSVRRGLAAFLALSLGVSTIVLVSSFDRSTIDMIAHAHKTRLAC
                     ALLLVLLVWAFDALKLYFVVKSAGERISGRLAILLNWLRYFGCAITPMQSGGGPFQVF
                     FLFKSGIPIGKGVAITLTSTLMTLFQLGFFVPLALLLQPELLQGRKLLQGVFSYVLVF
                     VLVSWTGVVISLLRPRLIKKWAGGITLLLKRWGFLKPVKVLRVVRRVNREIDNYNRNF
                     RVFFSSGMPLFALGFLFSCLQMVAMFSILPCLIWSLGMQVSFVEAFLAQALFLFILYF
                     VPTPGASGVAEGGGAAIFSLLVPWNIAGVVAITWRFFTEYLSIAMGGIVAVRLLGWGV
                     TDDLL"
     gene            complement(3329..3982)
                     /locus_tag="PLM30_03185"
     CDS             complement(3329..3982)
                     /locus_tag="PLM30_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR54207.1"
                     /translation="MEKTRVLLAAPQKLVREAIGKAIDNEEGLLLVGSTDDGLEAFRL
                     AREARPDVVVFDLGSGKMDWPHFVRELSLSCRGVRFLALSSDGSPDRISALCEAGIHG
                     CVSFSSGFSELVKGLRKVGEGDLFFDQEISSALPEILGKVNEGAILLRALTAREREVV
                     HLLSQGYSNQQIAEEMVLSEKTVKNHISHILRKLELKDRTQVAILAWKTGLALKGSL"
     assembly_gap    4217..4316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4317..4727
                     /locus_tag="PLM30_03190"
     CDS             <4317..4727
                     /locus_tag="PLM30_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HOR54208.1"
                     /translation="PCSLCSKMRAGALFAKAVEDGSTTVAFGHNMDDAVETALLNLFY
                     TGRFKCFHPRSWRSRTGLWLIRPLVFLSESEIAGEVRRLGLELMPPLCPFPRDSKRER
                     IKNLVRTLEEEIPDIRSQVLHALMWSGEKNHWED"
     gene            complement(4729..5589)
                     /gene="panC"
                     /locus_tag="PLM30_03195"
     CDS             complement(4729..5589)
                     /gene="panC"
                     /locus_tag="PLM30_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOR54209.1"
                     /translation="MSFVKPNIATTVAEARKFLKERSEAGGTIGLVPTMGFLHEGHLS
                     LVRRARSENDTCVVSIFVNPLQFGPSEDFDAYPRDTGRDLDLLASEGVKLVFAPGVKE
                     MFSPDHSTIVSEKKLSGFLCGSARPGHFDGVCTIVLKLFNILRPDRAYFGEKDYQQLQ
                     VIRRMVRDLDMEVEVIGCPIVREENGLALSSRNTYLSEAEREQAIALSRALAEVRSVF
                     ADGERDCRSILAVVRKVVGSYPMVRLDYAEVRDAEDLSPVERVDRKVVVALAARVGRA
                     RLIDNTVLAP"
     gene            complement(5577..>5944)
                     /locus_tag="PLM30_03200"
     CDS             complement(5577..>5944)
                     /locus_tag="PLM30_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOR54210.1"
                     /translation="WESLEELSYLGDKTMGSILDQEALSTASALARKGRPVFWLEVPV
                     IDAFHLGGLVFFFEYVTALTGLSLGLNPFDQPGVEQGKRYTYGLMGRSSFESDADEAR
                     IQFRSIMADSAGLLEDCHS"
BASE COUNT         1323 a         1695 c         1665 g         1061 t
ORIGIN      
        1 gaccagctta tagtatccgg ggtcgagagc catatctgtg tgctcgctac cgtgatggac
       61 ctgctgtcac agggatatcc cgtggccgtg gccgccgatg cctgctcaag tcgcgacccg
      121 gaccatcaca ggatggcttg cgaggccatg accgccgccg gggcgctggt ggcgcccgtt
      181 gagactttgg cttaccagtt gatccggcgg gcgggtacgg aacagttcaa agccatgctt
      241 ccttttttca aggcctgatc ggtcgagggt catcaaagtt cggggagggg aaccaatgcc
      301 gatagacagg acaagagcga tcgaactgca cagaaaggca agggggaaga taaagatcta
      361 ccctaccatc aacatccata acgaggagga aatggccctt gcctatatcc ccggaagtgt
      421 gccccccgcc cttgccatcc aacaggacga gggcatgagt tttgagtaca ccgggagggg
      481 caaccggatc gccgtcgtga ccgatggaag cgccgtcctg gggttgggaa atgtggggcc
      541 ctacgcggcc cttccggtca ttgaggggaa atgcctgctt ttcaagcttt tcggcgatgt
      601 gaatgcctac cccctgtgcg tgggctccca ggacacggaa gatatccttc acttctgcgc
      661 cctcctcgcc cctaccctgg gggggatcaa tatcgaggat atctcgagcc ccaaggcgtt
      721 caccatcgtc cgtgaactga ggaacaggat aaacatcccc gtcctctgcg atgaccaaca
      781 cgggacggcg gtcattgtac tggccgggat ctacaacgct ctcgaggtcc agggaaggaa
      841 actcgaggac atgaagatcg tgatcaacgg tgccggttcc gcggggatcg ccacggcgga
      901 gctgctcctc aagaccggcg ctaaggatat tgttgtgctg aacagcgccg ggatcctggg
      961 cgaggacaac ccaaggatga atcacaccca ggaagaactc gcggagatca cgaaccccgg
     1021 gaagataacc ggggggctcg ataaggccat ggaaggggcc aacattttcg tcgggctctc
     1081 caagggaggc gtccttcctc cgggaaacat caggctcatg tccggtgacc ctgtgatcct
     1141 ggccatggcg ctaccggaac ccgagatcct tccagaagag gcgatcgcag cgggggccgc
     1201 catcgtcgcg actggaggac ccggttacca gaacgccatg cccaactccc tgtccacgcc
     1261 ggggataatg cgagggctcc tcgacgtacg ggcaacggtg ctcaaccaca cgatgctcct
     1321 ggcagcggcc cgggcgctgg ccgacgtggt ggacaggcgc aggctgggcc cgggaaaaat
     1381 aattcccgac attttctgcg acgagactgc gccgagggtc gccgaggccg tcgggcaggc
     1441 cgcgatagct gagggcttcg ctacaaagga agtacccaag ggcgagatct ataacaatct
     1501 atggcaaaac ctctacgggg aacaaataac gagattttag ggattctggc ccgggaggtg
     1561 taagaggcat cggccttctt gcggcggtca ccctggagtt ccaaaaaaac ggctgttgtc
     1621 ccttcgttcc tggcgagagt gctctttctg tagtccggcg cccataaaag cggaaggtct
     1681 gccctaccac cagcgtcggg aaagcaagag caaagtcccg atgagggtga cccaaaggga
     1741 gtgacgttcg cttttccaga gaacgcccca gtcgaagggg cctttgagtt tcgccgggga
     1801 gggcgctctc aaagggaaga acctcccgac ccgctctgag taacgggcga attcgccggg
     1861 gaaacgctct ttcagataac cctcctcgta gggtattacc agaaggacat aaagggttat
     1921 gaaaacgacc aggaaaatca cgaaggccat cggctttccc gtaagggcgc accaccccgt
     1981 cccgatcagg gcattgccca ggtagagcgg gttcctggcc aaggcgtagg gtccccatgt
     2041 gactagggtg gccgcgccga cggtctcccc ccggtattgc ctgatcgacc ccgctgccca
     2101 gaacctgacc ccctgcccca gcgcgaccgc tgccaacccc gttgcgtgaa cgggcgtacc
     2161 gggacgcatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccagcagatc
     2281 atcggtcact ccccacccga gcaacctgac cgcgacgatg ccccccatcg cgatagagag
     2341 gtattcagtg aagaaacgcc aggtgatggc cacgacaccg gctatgttcc acgggacgag
     2401 aaggctgaag attgccgctc ccccaccctc ggcgactccg ctggctcccg gggtaggcac
     2461 gaagtagagg atgaaaagga aaagggcctg ggccagaaag gcctcgacga aactgacttg
     2521 catcccaagg gaccagatca agcaggggag aatggagaac atggccacca tctggaggca
     2581 ggaaaaaagg aaccccaggg cgaatagggg catgcctgag gagaagaaga cgcggaagtt
     2641 ccgattatag ttgtcgatct cacggttgac ccgcctcacg accctgagaa ccttcaccgg
     2701 cttcaggaac ccccaacgct tgagaagcaa ggtgatgccg ccggcccact tcttgatcaa
     2761 cctgggcctc agaaggctga tcacaacccc ggtccaggag accaagacga agaccaggac
     2821 gtaggaaaac acgccctgca ggagcttcct cccctgtagt agttccggct ggagcaggag
     2881 cgccaggggt acaaagaacc ccaactggaa tagggtcatc agggtgctcg taagggtgat
     2941 ggcaactcct ttgcctatgg ggatcccgct cttgaaaaga aaaaagacct ggaaagggcc
     3001 tcccccactc tgcatagggg ttatggcgca gccgaaatag cgaagccagt tcagaaggat
     3061 ggcaagtctc ccgctgatcc tctccccagc ggacttgacg acgaaataga gcttgagggc
     3121 gtcaaaggcc cacaccagga gcactaaaag cagagcgcaa gccaggcggg ttttgtgggc
     3181 atgggctatc atatcaatgg tagacctgtc aaaactggaa acaagcacga tggtgctcac
     3241 acccagggag agggcgagga atgcggccag tccccttcta acagacaagc tcactcatcc
     3301 cctgatggat catttctgcc gtgcccattc ataaggaccc cttcagggcc aaccccgtct
     3361 tccaggcgag gatggcgacc tgggtcctgt ccttgagttc cagttttctc aaaatatgac
     3421 tgatgtgatt ctttacggtc ttttccgaca ggaccatctc ctcggcgatc tgctggttgc
     3481 tgtacccctg agaaagaaga tggaccacct ccctctccct ggcggtgagc gcccggagca
     3541 aaatcgcccc ctcgttgacc ttgccgagga tctctggcag cgctgaagag atctcctggt
     3601 cgaagaaaag atcgccttcc ccaaccttgc gaaggccctt caccagttcg ctgaaccccg
     3661 acgaaaaaga gacgcagccg tgaatgccgg cttcacaaag ggcggagatc cggtcagggc
     3721 tcccatcgga agagagtgcc aggaagcgga cccccctaca ggacagggac agttctctca
     3781 caaaatgagg ccagtccatc ttgcccgatc ccaggtcgaa gaccaccaca tcggggcggg
     3841 cctccctggc cagacgaaag gcctcaaggc catcgtcggt cgacccgacc agcagtaggc
     3901 cctcttcgtt atcgatagcc ttaccgatgg cttcacggac cagcttttgg ggagccgcaa
     3961 gtagaacccg tgtcttttcc acaaaaacca cctccaggaa aaggctttgg cgggatagcc
     4021 cttgcgaagg atcgcgaaaa ggccccggtc ccctctccaa gccggtgtaa aatggcccca
     4081 gtgtaaggag gttgcgcagg agaggccagt cctcattaga gggaattata acctatcatc
     4141 gttaacggcg ctcgcgatca ttttatccca gcctggaggt acagcccgtg gaccgagcat
     4201 ttatcccgga tatatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt
     4321 gcagcctgtg ctcaaaaatg agggcgggtg ccctcttcgc caaggctgtc gaagacggct
     4381 ccaccactgt cgccttcggt cataacatgg atgacgcggt ggagacggcg ttgctcaacc
     4441 ttttttacac cgggcggttc aagtgcttcc atcctaggtc atggcggagc aggacaggac
     4501 tttggcttat caggcccctg gttttcctct ctgaatcgga gattgccggc gaggtccggc
     4561 gcctggggct ggagttgatg ccacctttgt gtcctttccc cagggacagt aaacgcgagc
     4621 ggatcaaaaa ccttgtccga accctggaag aagagatccc cgatatccgt tcccaggtac
     4681 tgcatgcctt gatgtggtca ggagagaaga accactggga ggactagttc aaggtgccag
     4741 aactgtgtta tcgatcagtc tcgccctccc gaccctcgcc gcgagagcca cgacgacttt
     4801 ccggtccacc ctttcgacag gggacaggtc ctcagcatcg cgcacctccg cgtaatcaag
     4861 tctgaccatg ggataagacc cgacgacctt cctcacgacg gccaggatag aacggcagtc
     4921 acgctccccg tcggcaaaga cggaccgcac ctccgccaag gcacgggaaa gagctatggc
     4981 ctgctctctt tcggcttccg acaggtaagt gttacggcta ctcagggcca gcccgttctc
     5041 ttcccgcacg atgggacacc cgatcacttc aacttccatg tccaggtccc taaccatacg
     5101 tcttatgacc tggagttgct gataatcttt ttcgccgaag taggccctgt cggggcgaag
     5161 gatgttaaaa agtttgagga cgatcgtgca gaccccgtcg aaatgaccgg ggcgggcgga
     5221 gccgcagaga aaaccgctca attttttctc cgatacgatc gtcgaatggt caggagaaaa
     5281 catttctttc accccgggag cgaagaccag tttcactccc tcggaggcta gcaggtcaag
     5341 atccctgccc gtgtccctcg ggtaagcgtc aaagtcctcg gaagggccga actggagagg
     5401 gttcacgaag atggagacca cgcaggtgtc attctcactc ctcgctcgcc ggaccaggga
     5461 gaggtggcct tcgtggagga atcccatcgt ggggacgaga ccgatggtgc ctccagcctc
     5521 acttctctcc ttcaaaaatt tcctcgcttc ggccaccgtt gtggcaatat tgggcttcac
     5581 gaatgacaat cctctaaaag gccggccgag tcggccatga tggaacggaa ttggatcctg
     5641 gcctcgtcgg cgtcgctttc gaaagaggac ctccccatca agccgtaagt ataccttttg
     5701 ccctgttcga cccccggctg atcgaagggg ttcagaccca acgacaaccc cgtaagggca
     5761 gtgacatatt caaaaaagaa caccagcccg cccaggtgga aagcgtcaat gacaggtact
     5821 tcaagccaga aaacaggacg tcccttcctg gcgagggcgg aagcggtcga tagggcctct
     5881 tggtcgagta tggagcccat ggtcttgtca ccgaggtaag agagctcctc caggctttcc
     5941 cagg
//
LOCUS       DAOSRG010000055         5829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_12544_length_5829_cov_7.784205, whole genome shotgun sequence.
ACCESSION   DAOSRG010000055 DAOSRG010000000
VERSION     DAOSRG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_5829_cov_7.784205"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLM30_03205"
     CDS             <1..464
                     /locus_tag="PLM30_03205"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOR54211.1"
                     /translation="EHLGSTDEELVRALALGLLVVGLIKSNTGILTPHCGCSVAAGAG
                     AAAAIVRLYGGTVNEMEAAVHLLLADITGMLCDGAKFGCSLKMATSSGVAFQSALLAM
                     KGASVPEKNGLVGRTLAETMRNLRVITDPGMAGVDRAVLEILTGGEDQGDC"
     gene            483..1508
                     /locus_tag="PLM30_03210"
     CDS             483..1508
                     /locus_tag="PLM30_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54212.1"
                     /translation="MTGESGRKWVEEHLKVGKEILYLTQEEVKNIGLSDGEILSLTEK
                     ALAAHGEKRTEMPAKIGLHPLKDTLMHAMPAYLPDYSACGIKWASCFPDNRERFGMPQ
                     TSGLLIYNDPESGWPLAVMDAVWITEKRTPAVTCVAAKHLAPAGVTTFGMIGCGVQGR
                     AHVGMIERVLVNLETIFIYDVFDEAALDLARDLQPIVKARIVRAGSIEEVVRSSQVVA
                     SATAITAQPRPRIRDSWVGRGQTILMCDMHSLYEDATMKRADKYLVDSIEEHELFVGY
                     GYYPDGLPEIYAETGEVAAGLKKGRENPEELIVCNNVGMAVEDMMVAKALFDRALSTG
                     AGKKLPL"
     gene            1533..2810
                     /locus_tag="PLM30_03215"
     CDS             1533..2810
                     /locus_tag="PLM30_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOR54213.1"
                     /translation="MPRLTDAEKSVLDGREGPLKRVALQFIVDYAEVMGAERLCDVTK
                     AHLFAGAHHYMDACTSDDIDEVVSEMLLCSTEKVSLDCFACYAQADVGPTDPVRWQQL
                     GVSPERHAKNSVILEKYTRAGLYPAATCTPYLSGFLPRMGEHYVSTESHAVTLMNSLW
                     GACANADGIEAAFCSAVCGKTPLWGNHIMSNRGGTHHFRVEFTPQNVMEWDLLGYVIG
                     SRTPTHSTPVLSGDLGTPGVVELKSCFASMATTGGAELCHIIGVTPEAPDFDRAFRGR
                     KAVAEEVITPRDLGEAAELFAGDGEVVDYVSLGCPHYSIDQVRDAASLLEGRKVHGDS
                     RLLVWTSPAVRELSDRAGYSRTIELSGARLVTSSCPLVSETWPRDASALAFDSLKQAH
                     YIRPETKAAVLFGSMGQCIDAAVTGRWERGYPR"
     gene            2807..3223
                     /locus_tag="PLM30_03220"
     CDS             2807..3223
                     /locus_tag="PLM30_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOR54214.1"
                     /translation="MRKKVIRGRGVWGGVAEGPAMVSRDTIQGWSGLDDETGVVIEKG
                     HPFEGLSIKGAVLILSGGKGSNGWSSHFHTARLKGLAPAAFVFPKMDSRTGVAVVVTK
                     VPAVTDLEEDVFEMIRTGDWVRVDGDSGTLEVTREG"
     gene            3267..4247
                     /locus_tag="PLM30_03225"
     CDS             3267..4247
                     /locus_tag="PLM30_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54215.1"
                     /translation="MKALWLSKKDVESLEIPMTEVMDAVEEGFRLNGLGQSELPAKIG
                     VHPRKDCFIHAMPCWMGGEVDAVGIKWAAGYPPNQVKGLPYINGVWCMNDPETGMIKA
                     VMDANWMTAWRTGAASGVCARHLASPGSEVVAMIGLGVQARTNLAALKEVLPGIREVR
                     IHDVFPEQAKRFQEDMAPLFSKAPLVIEDKAESAVKGADVVVTCTPIVEHPRRFVMRE
                     WMKEDVLCIAVDYDSAFREEVMKGDAFVCDNRNQYLWTQEQGVYFQDGYPLEAEIYAD
                     MGEICAGKAAPVRKGLRGAVLMGIASHDVMTGRLIYRKALEKNAGTWVEL"
     gene            4285..5397
                     /locus_tag="PLM30_03230"
     CDS             4285..5397
                     /locus_tag="PLM30_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOR54216.1"
                     /translation="MGGLKKFAVLGSGNGGMAFAAQIAAKGYPVVMFKPRDMTEDFIK
                     LNETREMFLEGDITCGGKILGVTNDMAEAARGTDVFFVVLPSFAHELVFEKLIPFLRD
                     GQHVVIVPGNFGGFLLKKMMADAGCKADVAVSETSSLPYACRTARFDTVMVYKRKFRL
                     KLGTCPVSKNEEALNIINDCFGGYIEFFPASSILEMDFDNVNYTLHPMPVLLNYGDIE
                     KNPKTFRHYMDGITPLVSEKMMQMDSERLAAGKKLGLELMPIMDQLKMYYGHNSTETI
                     YEYANSPESPYKDIVGHGVRSRYLTEDVPFSMVPCKFIGEKAGYPMPVVDLVIRMASF
                     LHDTDYVAQGHTLEKLGLDKMTLEELKEYARTGEVS"
     gene            complement(5462..>5829)
                     /locus_tag="PLM30_03235"
     CDS             complement(5462..>5829)
                     /locus_tag="PLM30_03235"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOR54217.1"
                     /translation="KGDRVILSNNPRIREGWRSCRWVDGDAAEVARQARDLIHRGWRF
                     HCHPLAGSIRLLRNPFRTMILDQGETPASPEQVAMAEEALRRLRAEGRGDVSKADEDD
                     YALIDLDLVVRALGGPDSE"
BASE COUNT         1197 a         1702 c         1874 g         1056 t
ORIGIN      
        1 cggaacacct cggttctacc gacgaggagc tggtcagggc ccttgccctg gggttactcg
       61 tggtggggct catcaagtcc aacacaggca tcctgacgcc ccactgcggc tgttccgtag
      121 cggcgggtgc cggggcagcg gccgccatcg tccgcctcta cggggggact gttaacgaaa
      181 tggaagctgc cgtgcacctg cttctggcgg atatcaccgg catgctctgc gacggcgcca
      241 agttcggttg ttcactgaag atggccacct cctccggggt cgctttccag tcggccctgc
      301 tggccatgaa gggggcgtcg gtccccgaaa agaacggcct ggtggggaga accctggctg
      361 agactatgag gaacctcagg gtcatcaccg acccgggaat ggccggcgtc gatcgggctg
      421 tcctcgagat cctgacgggc ggtgaagatc aaggtgactg ttgagaggag cggacatcca
      481 ttatgactgg tgaaagtgga aggaaatggg ttgaagaaca cctgaaggta gggaaggaga
      541 tcctttacct tacccaggaa gaggtgaaaa atatcggtct ttccgacggt gagatcctgt
      601 ccctaaccga gaaagccctc gcggcccacg gcgagaaaag gacggagatg cccgccaaaa
      661 taggcctgca cccattaaag gacaccctca tgcacgccat gcccgcctac ctgccggact
      721 actcggcctg cgggataaag tgggcttcct gcttccccga caaccgggag cggttcggga
      781 tgccccagac ttccgggctc ctgatctaca acgaccccga gagcgggtgg cccctggccg
      841 tgatggatgc cgtttggatc accgaaaaaa ggaccccggc ggtgacctgt gtcgccgcga
      901 aacacctggc gcccgcgggg gttacgacct tcgggatgat aggttgcggc gtacagggga
      961 gggctcacgt gggaatgatc gaaagggtgc tggtgaacct ggagacgatc ttcatctacg
     1021 acgtcttcga cgaggcggcc cttgatctgg cccgtgacct gcaacccatc gtcaaggcga
     1081 ggatcgtgag ggccggatcc atcgaggaag tggtccgatc ctcccaggtc gtagcgtcgg
     1141 ccacggcgat cacggcccag ccaaggccca ggatcaggga cagctgggtc gggcggggac
     1201 agactatcct catgtgcgac atgcactccc tttacgagga cgccactatg aagagagccg
     1261 acaagtacct cgtcgacagc atcgaggaac acgaactttt cgtggggtac ggctattacc
     1321 ccgacggcct cccggagata tatgccgaga ccggcgaggt ggccgcgggc ctgaaaaagg
     1381 gcagggagaa ccccgaggag cttatcgtct gcaacaacgt gggtatggcc gtggaagaca
     1441 tgatggtggc gaaggctctc ttcgacaggg ccctctccac cggtgccggc aaaaaactac
     1501 ccctttaacc gggttgtagg ggaggcgatc ctttgcccag gcttaccgat gcggaaaagt
     1561 cggttctcga cggccgagag ggtccactca agcgggtggc cctccagttt atcgttgatt
     1621 acgccgaggt gatgggagcg gaacggctct gcgacgtcac caaggcccac ctcttcgcgg
     1681 gggcccatca ctacatggat gcctgcacct ccgatgatat cgatgaggtg gtctctgaga
     1741 tgcttctctg ttccaccgag aaggtcagcc tggattgctt cgcctgttac gcccaggccg
     1801 acgtggggcc cacggacccg gtcaggtggc agcagctggg agtatcgccc gagagacacg
     1861 ccaagaacag tgtgatcctg gagaagtata cgagagccgg cctctacccg gcggccacct
     1921 gtacacccta cctttcaggc ttcctcccca ggatgggcga gcactacgtc tccaccgaat
     1981 cccacgccgt gacgctcatg aactccctct ggggggcctg cgccaacgcc gacgggatag
     2041 aggccgcctt ctgttccgcc gtctgcggga agacgcccct ttgggggaac cacatcatgt
     2101 cgaacagggg agggacccat cacttcaggg tggagttcac cccacagaat gtcatggagt
     2161 gggacctgct gggttatgtc atcggatcca ggacccctac ccactccaca ccggtccttt
     2221 cgggcgacct gggtacaccc ggcgtggtgg agctgaaatc gtgcttcgct tcaatggcga
     2281 ccacgggagg agcggagctg tgccacatca taggggtgac ccccgaggcg cccgatttcg
     2341 accgggcctt cagaggccgg aaagccgtcg cggaagaggt gatcacccca cgggatctcg
     2401 gggaggcggc ggaactcttt gccggtgacg gcgaggtagt ggattatgtc agcctgggtt
     2461 gtccccatta ttccatcgac caggtcaggg acgccgcgtc ccttctcgag gggcgcaagg
     2521 tccacggcga tagccggctt ttggtatgga cctcgccggc ggtgagggag ctctccgaca
     2581 gggccgggta cagccggacg atcgagctct ccggcgccag gctcgtcacg agttcatgcc
     2641 ccctggtcag cgaaacctgg ccgagggatg cctccgccct tgccttcgac tcgctgaagc
     2701 aggcccatta catcaggccc gagacgaagg cggcggtcct cttcggctcc atggggcagt
     2761 gcatcgacgc ggccgttact ggccggtggg agagggggta tccccgttga ggaagaaagt
     2821 catcaggggc agaggcgtat ggggcggtgt cgccgaaggc cccgccatgg tcagcaggga
     2881 tactatacag ggctggagcg gcctcgacga tgagaccggc gttgtcatag aaaagggtca
     2941 ccccttcgaa ggcctgtcca tcaagggcgc cgtcctgatc ctatccggcg gaaagggttc
     3001 caacggctgg tcgagccact ttcacacggc caggctcaag ggccttgccc cggcggcgtt
     3061 cgttttcccg aagatggatt cccgcaccgg tgtggcggtg gtggtgacga aggtccccgc
     3121 cgtgaccgac ctggaggaag atgtatttga aatgatcagg accggtgact gggtccgcgt
     3181 agacggcgac agcggcaccc tggaggtcac ccgcgaagga taaaccgaaa ggggcggttt
     3241 cccccacttg aggaggaggt aaaaggatga aagctctttg gctctcaaag aaggatgtgg
     3301 aatccctgga gatacccatg acggaggtca tggatgccgt cgaagaaggg ttccggctta
     3361 acgggctcgg ccagagcgaa ctgccggcca agataggggt ccaccccagg aaggactgct
     3421 ttattcacgc tatgccctgc tggatgggcg gcgaggttga cgcggtaggc ataaagtggg
     3481 ccgccggcta cccccccaac caggtcaagg gtcttcccta tatcaacggc gtttggtgca
     3541 tgaacgaccc cgaaacgggg atgatcaagg ccgtaatgga tgcaaactgg atgacggcct
     3601 ggaggaccgg cgccgcgtcg ggcgtgtgcg cccgccatct cgcctcgccg gggtcggagg
     3661 tggtcgccat gatcggcctt ggagtccagg cccggaccaa cctggccgcg ttgaaggagg
     3721 tcctccccgg gatcagggaa gtccgcatcc atgatgtctt tcccgaacag gcgaagcgct
     3781 tccaggaaga catggcccct cttttctcga aggcccccct cgtcatagag gacaaggccg
     3841 aatcggccgt aaagggggcc gatgtggtgg tcacctgcac ccccatcgtt gaacaccccc
     3901 gccgcttcgt catgagggag tggatgaagg aggacgtgct ctgcatcgcc gtcgactacg
     3961 attccgcttt ccgtgaggag gtaatgaagg gcgacgcttt cgtctgcgat aacaggaacc
     4021 agtacctctg gacccaggag cagggcgtct acttccagga tgggtacccc ctggaggcgg
     4081 agatctacgc agacatgggg gagatctgcg cagggaaggc cgccccggtg aggaagggcc
     4141 tcaggggtgc ggtcctgatg ggcatagcca gccatgacgt gatgaccggg cgcctgatct
     4201 atcggaaggc ccttgagaag aacgccggca cctgggtcga gctttgatgc cttcaggatc
     4261 agaaaaaagg aggttgatca ttcgatgggc ggactaaaaa agtttgcggt ccttgggagc
     4321 ggaaacggag ggatggcctt tgcggcgcag atagccgcga agggataccc ggtggtgatg
     4381 ttcaagcccc gggatatgac agaggatttc atcaaactga acgagaccag ggagatgttc
     4441 ctcgagggcg acatcacctg cggcggcaag atcctcggtg tcaccaacga tatggccgag
     4501 gcggcgaggg ggaccgatgt cttcttcgta gtgcttccct ccttcgccca tgaactggtc
     4561 ttcgaaaaac tcatcccctt cctgagggac ggacagcacg tggtcatcgt accgggcaac
     4621 ttcggcggct tcctgctcaa gaaaatgatg gccgacgccg gttgcaaggc cgatgtggcc
     4681 gtctcggaga cctcgagcct cccctacgcc tgccggaccg cgcggttcga tacggtgatg
     4741 gtctacaaga gaaaattcag gctgaagctg ggcacctgcc cggtttcaaa gaacgaagag
     4801 gctcttaaca tcattaacga ctgtttcggc gggtatatcg agtttttccc ggcgtcgagc
     4861 atcctggaga tggatttcga taacgtcaac tacaccctgc acccgatgcc ggtcctgctg
     4921 aactacggag atatcgagaa gaaccccaag accttccggc attacatgga cgggatcacg
     4981 cccctggtct cggagaagat gatgcagatg gattctgagc gcctggccgc cgggaagaag
     5041 ctcggcctcg aactcatgcc gatcatggac cagttgaaga tgtactacgg tcacaattct
     5101 acggagacga tctacgagta cgcgaacagc cccgagagcc cctacaagga catcgtcggg
     5161 catggcgtga ggagcaggta tctgacggag gacgtcccct tctcgatggt cccctgcaaa
     5221 ttcatcgggg agaaggcggg ataccccatg cccgtggtgg acctcgtcat aagaatggcg
     5281 tccttcctcc acgacacgga ttatgtcgcc caagggcaca cgctggaaaa actcggcctc
     5341 gataagatga ccctggaaga actgaaggaa tacgccagga ccggcgaggt ttcctgaagg
     5401 atatcccggc ccggatgata agcgataaac tcaggccccg gtccaggacc ggggcctgat
     5461 cctattcgct gtcggggccg cccaaggccc taaccaccag gtcgagatct atgagggcgt
     5521 agtcgtcctc gtcagccttg gatacgtcgc cgcgaccttc ggcacgaagc ctccggagtg
     5581 cctcttcggc catggctacc tgttcgggtg aggcgggggt ctcaccctga tcgaggatca
     5641 tggtcctgaa gggatttctg agtagccgga tgctccccgc cagggggtgg cagtgaaacc
     5701 gccacccacg atggataagg tcccgagcct gccttgccac ctcggcggcg tcaccgtcga
     5761 cccagcggca ggaccgccac ccctccctga tcctggggtt gttgctcagg atgacccggt
     5821 cgcccttca
//
LOCUS       DAOSRG010000056         5700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_12880_length_5700_cov_9.796457, whole genome shotgun sequence.
ACCESSION   DAOSRG010000056 DAOSRG010000000
VERSION     DAOSRG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12880_length_5700_cov_9.796457"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1250)
                     /gene="selD"
                     /locus_tag="PLM30_03240"
     CDS             complement(216..1250)
                     /gene="selD"
                     /locus_tag="PLM30_03240"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1215..1217),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOR54218.1"
                     /translation="MRLTELSHSSGUAAKIGPADLKEILKALPPQENGFLFTSWDKGE
                     DAATWMIDGKRTAIMTLDFITPVVDDPFTYGQIAAANALSDVYAMGGRPLVALNIVGF
                     PVTCEPLEVLSEILKGGQERVNAAGAVLAGGHSVDDGEPKYGLAVFGEVDAAKMWKVT
                     GVRPGDALILTKPLGTGLVATAIKAGAADPGETEAAVRSMRALNDLPARLPGELLAEV
                     HACTDVTGFSLAGHLLDMLSDGGLEARLSPGELPLLPGALEKAEMGFVPGGSNRNMEL
                     YAPRVEGLEKMPGALPALLFDAQTSGGLLLAVSEGAAGRFLEACHEAGFPHAAKIGVF
                     VEGPERIRIW"
     gene            complement(1501..2433)
                     /locus_tag="PLM30_03245"
     CDS             complement(1501..2433)
                     /locus_tag="PLM30_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR54219.1"
                     /translation="MARPLRIEYPGALYHVMSRGNEKKDIFRTTRDREKFLSYLSSAW
                     ERHGAVFHAYCLMSNHYHLMVETPDGNLSRVMKHINGSYTTYFNLKHQRAGHLLQGRY
                     KATLVEADAYAAELSRYIHLNPVRAKITSSPEEYHWSSCPRYLDGSAPSWLSTGLVLG
                     YFGPSEKDRPGNYRAYLMEAVGEEGVDPLAGSIASTILGTDEFIRDIREKYLEGMEPG
                     RDLPALRELVGGPALEDIKAASEEVFPGEDKLARMAGIYLCWRFSGAKLKEIGDLYGL
                     TESGVNRACRRLEGRMEKDRELKERVREIGEKLK"
     gene            complement(2528..3832)
                     /locus_tag="PLM30_03250"
     CDS             complement(2528..3832)
                     /locus_tag="PLM30_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOR54220.1"
                     /translation="MSQKSAGTVFVDRFTDGVLDPSKPMLGPVKDGGVIVANTAPGCW
                     GPMITPELKGGHEVTLPVAVEGSEPGDAIAIRIKDVVVTSLATASGTDVTPDGSQFRG
                     DPFVAKQCPSCGAYRPKTVLEGTGRGAVRCAHCGAVISPFVMSNGYTIVFDHERELAV
                     TLDESGAEKAAGDADFLSAKPDHSVQHSILVFAPADIHGMATRMRPFLGQLGTMPCLP
                     FPDSHNAGDFGAFLVGAPHEYAKTKEELECRTDGHMDVNTVRAGAVLICPVKVPGGGV
                     YLGDMHAFQGNGEIAGHTADVAGVAALQVHLLKGLKIDGPILLPLEEDLPHLAKPLTP
                     TEKEGLKSLARKWGLEGIEEDAPICFIGTGSAMNDAISNGLERASTVLGMALDELRNR
                     VTVAGAIEIGRAPGVVMVTFRCPIEKLERLKIMSFVRQQYSL"
     gene            4211..5698
                     /locus_tag="PLM30_03255"
     CDS             4211..5698
                     /locus_tag="PLM30_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOR54221.1"
                     /translation="MSSGFTAARNRSKYISPQSGMCSLCTEECPGTCEIAQAAVLGKI
                     TVYPTTTGANQIASEKDYPIDFSHFNINGRAFGALGVEADLEHGEIFNVNLASEYGYD
                     NKVKLDLPIVLPAIIKMNWVDYFGGAAMAGVTCMIGEDAREKEPNLEMTDGKITSFPM
                     LQKIHDSFHKYYRGSGQMVLQCNVDDHLTGVPEIALEKYGFEALEFKFGQGAKGTQPV
                     NRIKNYDEAVKKVEKGHLVHPDPFDPKVVELREKGCCPNFYTYGRLPMWTEETMAKRI
                     AELRERGTKNVYFKMAGFDLADVERVIRMAIENRVDMVTFDGAGGGSGYSPSKMMDEW
                     SLPTVMLEKGVVDICDKLERQGKTLPAMVITGGFISEDQVFKALAYGDGFINAVGVCR
                     GAMAAAMTGKTIGDEIKAGRTPEFFKKFGDTVDEVFRDLADLKGIYGKEAENFSTGAI
                     GVFSYLNKLAFGLRHFAALNRKFDLMYLDRSDLIPLTYYANDLLG"
BASE COUNT         1126 a         1782 c         1637 g         1155 t
ORIGIN      
        1 gcgagtcgag gtagaaatcc ctggcctcga gctccatcct catggcgaaa tccacgatcc
       61 gctccgcctt tttcatttgc cgcacccccg acatgatatt gtgccagtct tttgattcta
      121 tcagtaagga agggggtaaa aagcgcggcg gcctttggat aatggataca aaaaagcggc
      181 ccccgaccac atcgggagcc gccctggtcc ggtccttacc agatccttat cctttcaggc
      241 ccttcgacga aaacaccgat cttagccgca tgagggaacc cggcttcatg gcatgcctcg
      301 aggaaccgcc ccgcggcccc ctcgctcaca gccagcagga gcccgcccga ggtctgggcg
      361 tcgaaaagca gggctggaag ggccccgggc atcttctcga ggccctcgac cctcggggcg
      421 tatagttcca tgtttcgatt ggagccgccg gggacaaagc ccatctcggc cttctcgagc
      481 gccccaggca gcaggggcag ttcaccaggg gagagcctcg cctccaggcc tccatcagag
      541 agcatgtcca ggaggtgccc cgccaggctg aaacccgtca cgtcggtgca ggcgtggacc
      601 tccgccaaca gttcaccggg cagcctcgcc gggagatcgt tgagggcgcg catggacctg
      661 acggcggctt ccgtctcccc ggggtcggcc gccccggcct ttatggccgt cgctaccagg
      721 cccgtgccca ggggctttgt gaggatgagc gcgtcgccgg ggcggactcc cgtgaccttc
      781 cacatcttcg cggcgtcgac ctcgccgaag accgcgaggc cgtacttggg ctctccgtcg
      841 tcgacgctgt ggccgccggc gaggacggcc ccggccgcgt taaccctctc ctgaccgccc
      901 ttgaggatct ccgacagcac ctccagcggt tcgcaggtga cgggaaagcc gacgatgttc
      961 agggccacca gcggcctacc gcccatggcg tagacatcgc tcagcgcgtt ggcggcggcg
     1021 atctggccgt aggtgaaggg gtcgtccacc acgggagtta tgaaatcgag ggtcatgatg
     1081 gccgtcctct tcccgtctat catccaggtg gcagcgtcct cgcccttgtc ccaactggtg
     1141 aacaggaacc cgttctcctg agggggaagc gccttcagta tctctttcag gtccgccgga
     1201 cctattttgg ccgctcagcc gctgctgtgc gacagctcag taagccgcat ggtgaaacct
     1261 cctcattgtg gggtcaggtc tacacttttg actatttcag cttttcccct atttcacgtc
     1321 ctcattgggg tcaggtctac acttttgact atttcagctt ttcccctatt ttccggaccc
     1381 tttccttcaa ctccctgtcc ttttccatcc tgccttcaag cctccggcag gcgcggttca
     1441 cccccgattc cgtcaacccg taaaggtccc ctggcaccag ggtcaggtct acacttttga
     1501 ctatttcaac ttttccccta tttcccggac cctttccttc aactccctgt ccttttccat
     1561 cctgccttca agcctccggc aggcgcggtt cacccccgat tccgtcaacc cgtaaaggtc
     1621 ccctatctct ttaagcttag caccactgaa cctccagcag aggtagatgc ccgccatcct
     1681 tgccagcttg tcctccccgg ggaaaacctc ttcagaagca gccttgatat cctccagggc
     1741 ggggccgccg accagttccc tcagcgccgg aaggtcacgg cccggctcca tgccttccag
     1801 gtacttctcc ctgatgtccc tgatgaactc gtcggtgccg aggatcgtcg aggcgatcga
     1861 accggcaagg gggtccacac cctcttcgcc gacagcctcc atcaagtagg ccctgtagtt
     1921 tccggggcgg tccttttcgg acgggccgaa ataccccagg acaaggccgg tcgaaagcca
     1981 ggagggggcg gagccgtcga ggtaccgggg acagctgctc caatggtact cctccgggga
     2041 tgaggttatc ttcgccctga cggggttcag gtggatgtag cgcgaaagtt cagccgcgta
     2101 ggcgtcggcc tcgaccaggg tcgccttgta gcggccctgg agtaggtgac ccgccctctg
     2161 atgcttcagg ttgaaatagg tggtgtagga cccgttgata tgcttcatca cacgcgagag
     2221 gttcccgtcg ggggtctcca ccatcaagtg gtagtggttg ctcatgaggc agtaggcgtg
     2281 gaacaccgcc ccatgcctct cccaggccga cgacaggtac gaaaggaatt tctccctgtc
     2341 ccgcgtggtc ctgaagatat ccttcttttc attgcctcgg gacatcacgt ggtacaaggc
     2401 gccggggtat tcgatcctca aaggcctggc caaggggaaa acctcccgtc gggtaaggga
     2461 cgatcaagat tttccccgat tacgccggat tagtcaaaag tgtagacctg accccgctcc
     2521 tggtctttta gaggctgtac tgctgacgca cgaaggacat gatctttaat cgctccagtt
     2581 tttctatcgg gcaccggaaa gtcaccatca ctacccctgg cgctcggcct atttcgatcg
     2641 ccccggcaac ggtgacccgg ttcctcaact cgtccaaggc catgccaagc acagtggatg
     2701 ctcgctccaa cccgttgcta atggcatcgt tcatagcgga tcccgtgccg atgaagcaga
     2761 tcggcgcatc ctcctcgatc ccctcaagcc cccatttccg cgccaacgac ttcagcccct
     2821 ctttttcggt aggggtcaaa ggcttagcca ggtgcggaag gtcctcctcc agcggcagca
     2881 aaatgggccc gtcgatctta agacccttca gtaggtgaac ctgcagcgcc gccactcccg
     2941 ctacgtcggc ggtatgcccc gcgatctccc cgttcccctg gaatgcgtgc atatccccca
     3001 aataaacgcc cccgcctggg accttcaccg ggcagatgag caccgccccg gcccgaacgg
     3061 tgttaacgtc catatggccg tcggtcctgc actccagttc ctccttcgtc ttggcgtact
     3121 catggggagc ccccacgaga aaggcgccga agtcaccggc gttgtgcgag tctggaaagg
     3181 gcaggcaggg catggtcccc aactggccaa ggaagggcct catcctagtt gccatgccgt
     3241 ggatgtccgc cggggcgaag acgaggatgg aatgctgtac agagtggtcc ggcttcgctg
     3301 aaaggaagtc tgcgtccccg gctgcctttt cagccccgct ttcgtccagc gtcaccgcca
     3361 actcccgctc gtggtcgaag acgatagtgt acccgttgct catgacgaag ggtgagatca
     3421 cggcaccgca atgggcacac cggaccgctc cccggcccgt tccctcgaga accgttttcg
     3481 gtcggtaggc cccgcaggaa ggacactgct tcgccacaaa ggggtccccc ctgaactggc
     3541 tcccgtccgg ggttacatcg gtccccgatg cggttgccaa ggatgtcacc accacatcct
     3601 ttatccggat ggcaatggcg tcaccgggtt ccgagccttc cacggccacc ggcagggtaa
     3661 cctcgtggcc tcctttaagt tccggcgtga tcatgggccc ccagcacccc ggcgccgtgt
     3721 tcgccacgat gaccccgccg tccttcaccg gccccagcat gggcttcgaa ggatcgagca
     3781 ccccatcggt gaaccggtcc acaaacaccg ttcctgcact tttttgactc atgactcaac
     3841 actcctttcc gggccatgcc ggagtcttcc tggcttggca aaacaccttt ggagtggcga
     3901 gagcgggtga gatcagggga aaaagcaaag tggcgggtta tttatgcgac ctctaaggct
     3961 agaagatcga agggcttttt atattctaac cctttcccgg gggcgaggaa taatggtttc
     4021 gagattcctg gggaaaccct ttttacggtt ctataaggat tgttctctgg ataaggggat
     4081 tcccgaggac ctcctttttc tggcggcacg atttaagcca agtgatacaa ttatcttgaa
     4141 gggcatgaag ggataacaag agatagatta aaccacgacg ggaggttcgg tcatgacctt
     4201 ttcacccagt atgagttcgg gctttacggc ggccaggaac aggagcaagt atatttcccc
     4261 ccagtcgggg atgtgctccc tgtgcaccga ggagtgcccg gggacttgcg agatagccca
     4321 ggcggccgtc ctggggaaga taacggttta tcccaccacc accggcgcca accagatcgc
     4381 gtcggaaaag gattacccca tcgatttttc ccatttcaat ataaacggcc gtgctttcgg
     4441 cgccctggga gtggaggcgg acctcgagca cggggagata ttcaacgtca acctggcctc
     4501 ggagtacggc tatgacaaca aggtgaaact cgacctgccc atcgtcttgc ccgccatcat
     4561 caaaatgaac tgggtcgatt acttcggggg cgccgccatg gcgggggtga cctgcatgat
     4621 cggcgaggac gccagggaga aagagcccaa cctggaaatg acggacggca agatcacgtc
     4681 ctttcccatg ctgcagaaaa tacacgacag cttccataaa tattaccggg gttcggggca
     4741 gatggtgctc cagtgcaatg tcgacgacca tctcacgggt gttccggaga tagcgctgga
     4801 aaaatacggc ttcgaggccc tggagttcaa gttcggccag ggtgccaagg ggacccagcc
     4861 ggtcaacagg atcaagaact acgacgaggc cgtgaaaaag gtggagaagg ggcaccttgt
     4921 ccaccccgac cccttcgacc ccaaggtggt ggaactcagg gaaaagggtt gctgccccaa
     4981 cttctacacc tacggaaggc tcccgatgtg gaccgaggag accatggcga agcggatcgc
     5041 cgagttgcgg gaaaggggta cgaagaacgt ctacttcaag atggccggct tcgacctggc
     5101 cgacgtggag cgggtcatcc gcatggcgat agagaacagg gtcgacatgg tcaccttcga
     5161 cggggccggc ggcggttccg gctacagccc ctcgaagatg atggacgaat ggtccctccc
     5221 gacggtcatg cttgaaaagg gtgtcgtcga tatctgcgac aaactggaaa ggcagggcaa
     5281 aacgctgccg gccatggtca tcaccggggg cttcatcagc gaggaccagg tattcaaggc
     5341 cctcgcctac ggcgacgggt tcatcaacgc cgtcggcgtc tgccgcggcg cgatggcggc
     5401 ggccatgacg ggcaagacca tcggtgacga gataaaggcg ggcaggactc cggaattctt
     5461 caaaaagttc ggcgataccg tcgacgaggt cttccgcgac ctggccgacc ttaaggggat
     5521 ctatgggaaa gaggccgaga acttctccac gggggcgatc ggggttttct cctacctgaa
     5581 caagctggcc ttcggtttga ggcatttcgc ggccttgaac cggaaattcg accttatgta
     5641 cctggacagg agcgacctga tcccgctcac ctactacgcc aacgacctgt tgggctagat
//
LOCUS       DAOSRG010000057         5461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_13629_length_5461_cov_8.386053, whole genome shotgun sequence.
ACCESSION   DAOSRG010000057 DAOSRG010000000
VERSION     DAOSRG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13629_length_5461_cov_8.386053"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLM30_03260"
     CDS             complement(<1..283)
                     /locus_tag="PLM30_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR54222.1"
                     /translation="MPNSDAVLETEALTIRFGGLVAVRDFSVRIPRGSIVGLIGPNGA
                     GKTTCFNMVTGFYKPTEGKILFNGQDITGWLPHKVCSAGIARTFQNIRLF"
     gene            complement(283..1329)
                     /locus_tag="PLM30_03265"
     CDS             complement(283..1329)
                     /locus_tag="PLM30_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54223.1"
                     /translation="MDPKYKRNYAFNGIAVLLLVVALFWSRGHLDGYKIQIMNLIAVN
                     IILALSLNLIYGFTGLFSLGHAGFMAIGAYTCSLLILPATYKQTMFLLQAPMWPFSVM
                     HAPFFVAVIIAGLAAAFFGFLIGLPVLRLGGDYLGIATLGFAEIIRVLANNMPRVTNG
                     ALGLKGIPSYSNLWWNYGWCLLTLYVMVRLVRSNFGNALKAIRDDEVAARAMGINTFR
                     YRLTSFTIGAFFAGVGGALLASLLTTIDPRMFIFTLTFNVLMIVVTGGLGSITGTILA
                     GIVITVLLEWLRFVENPITLAGMTVPGVPGMRMVVFSLALLFIILFRHQGIMGRREFS
                     WDWLFGLFRKRREA"
     gene            complement(1329..2222)
                     /locus_tag="PLM30_03270"
     CDS             complement(1329..2222)
                     /locus_tag="PLM30_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54224.1"
                     /translation="MSLAMFVQHLLNGLTLGSLYALIAIGYTMVYGILRLINFAHGEI
                     FMLGAYFVFWGVTLFRLPWFIAVIGAIVVTSFAGIIVDRVAYKPLRNAPRISALISAI
                     GVSFFLQNFAIVVFSAIPREVQRPAWLVKVIIVGKVRILPLTLVVPALSFVLMMGLLY
                     VVYKTKPGLGMRAISRDIETSRLMGVPVNRIIALTFGMGSALAAASGIMWALRYPQLQ
                     PVMGVIPGFKAFIAAVFGGIGSIQGAVIGGLILGLMEIMIVAFMPSLAGFRDAFAFIL
                     LIIILLIMPTGLMGEKLEDKV"
     gene            complement(2330..3559)
                     /locus_tag="PLM30_03275"
     CDS             complement(2330..3559)
                     /locus_tag="PLM30_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54225.1"
                     /translation="MKKGLVLGVIVVIAVIGFMMIQGQKAPEAPAPEPAAEAPAPEPA
                     EEPKEEQAAVESGVIHVGVYLPLTGQNAFGGQLELDGVKMAHEEKPEVLGRKVELSVV
                     DNKSDKVESANAVKRLIERDNAVAVIGSYGSSLAMAGGEVAESAGIPMVGTSCTNPLV
                     TQGKKYIFRVCFIDPLQGAGAATYAYKVIGARKAALLTDVANDYAVGLAGFFEKSFLK
                     LGGEIVAKLNYNSGDQDFTAQLTEIISKEPDIMFIPAYFAEGAIIMKQAQELGVDFQI
                     MGGDAMDNPKIVEIGGDAVEGFVHTTFPYDPSMKEMNPVARRFTDNWLAKRSDDPNVN
                     AALGYDSYMILIDAIERAGSADPEAITAALAATKDFEGVTGLTTINETHDAEKPIGIV
                     QIKDGKKQYIATITPEL"
     gene            complement(3818..4288)
                     /gene="nrdR"
                     /locus_tag="PLM30_03280"
     CDS             complement(3818..4288)
                     /gene="nrdR"
                     /locus_tag="PLM30_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOR54226.1"
                     /translation="MRCPKCGLMETRVLETRTAGEGRVIRRRRECPGCQYRFTTYERF
                     EDRPVLWIVKKSGQREAFDRGKLLKGFAKACEKLPVSLEQIEEAVSRIEEALRESGQG
                     EVSSLTVGSLAMAELRDIHKVAYVRFASVYREFTDVSSFVEEISRLRDERNGHR"
     gene            complement(4493..4672)
                     /gene="rpsU"
                     /locus_tag="PLM30_03285"
     CDS             complement(4493..4672)
                     /gene="rpsU"
                     /locus_tag="PLM30_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR54227.1"
                     /translation="MTQVVRRDNESLEDALKRFKREVSKGGILREARRRKHYEKPSEA
                     KKRKREDASRKRRGR"
     gene            complement(4704..5045)
                     /locus_tag="PLM30_03290"
     CDS             complement(4704..5045)
                     /locus_tag="PLM30_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOR54228.1"
                     /translation="MDHECPFCRIVQGRGKADILYSDNDVVVIRDIHPKAPVHLLVIP
                     KRHVGSADDIEDPSTWSTVMDAAVKVARELDLREGGYRLVVNCGAGAGQTVNHLHVHL
                     LAGRVFGWPPG"
     gene            complement(5121..>5461)
                     /locus_tag="PLM30_03295"
     CDS             complement(5121..>5461)
                     /locus_tag="PLM30_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOR54229.1"
                     /translation="MLERTRVGKLHAFPFSAREGTPASNFEKPIPPDEIRSRMDRALM
                     TGYELLGLYSRRWVGKEVEVLVERNQEGKISGLSRHYLRVETRGDPRPGEIATLTIKD
                     SVKGALIGLV"
BASE COUNT         1114 a         1665 c         1525 g         1157 t
ORIGIN      
        1 ggaaaagcct tatgttctgg aaggtcctgg ctatccccgc cgaacatacc ttgtgtggga
       61 gccatcccgt gatatcctgc ccgttgaaaa ggatcttccc ctccgtcggt ttgtaaaaac
      121 ccgtcaccat gttgaagcag gtggtctttc cggcgccgtt cggcccgata aggccgacga
      181 tgctgcccct ggggatacgc accgagaagt ccctgacggc gactaggccg ccgaaacgta
      241 ttgtgagggc ctctgtctcg aggacggcgt cactattggg catcaggctt ctctcctttt
      301 ccggaagagg ccgaagagcc agtcccagct gaactcacgc ctccccatga tcccctggtg
      361 cctgaaaagg atgatgaaca ggagtgccag ggagaacacc accatacgca tgcccggaac
      421 ccctgggacc gtcatgccgg cgagggtgat cgggttctct acgaatcgga gccattcgag
      481 caggacagtg atcacgatgc ccgccaggat cgtgccggtg atcgaaccaa gtccccccgt
      541 caccacgatc atcagcacgt tgaaggtcag ggtgaaaatg aacatcctcg ggtcaatggt
      601 cgtaaggagg cttgccagca aggccccgcc gacgccagcg aagaaggcgc cgatcgtgaa
      661 ggatgtcagc cggtaacgga atgtattgat ccccatggcc cgggccgcta cctcgtcgtc
      721 cctgatcgcc ttgagggcgt tgccgaagtt gctccgcaca aggcggacca tcacgtagag
      781 cgtcaagagg caccagccat agttccacca gaggttcgaa taggaaggga tgcccttcaa
      841 tcccagcgcg ccgttggtga cccggggcat gttgttggcc agcaccctta tgatctcggc
      901 gaagccgagg gtcgctatcc cgagatagtc ccccccgagc ctgaggacgg gaaggcctat
      961 cagaaaaccg aaaaaggcgg ccgccaggcc ggcgatgatg acggccacga agaagggtgc
     1021 gtgcatcacc gaaaaaggcc acatgggggc ctggaggagg aacatggtct gtttgtacgt
     1081 ggcgggcagg atgagaaggg agcaggtata ggccccgatg gccatgaaac cggcgtggcc
     1141 cagcgagaaa agtccagtga acccgtagat gaggttcagg ctcagggcga ggatgatgtt
     1201 gaccgctata aggttcatta tctggatctt gtaaccgtcc aggtggcccc tggaccagaa
     1261 aagggcaacg accagaagga gaaccgcgat cccgttgaag gcgtaattcc ttttatactt
     1321 cgggtccatc acactttatc ctccagcttc tcacccatga gccccgtagg catgatgaga
     1381 aggatgatga tcagaagaat gaaggcgaag gcgtccctga aaccggccag ggaaggcatg
     1441 aaagccacga tcatgatctc catcaaacct aggatcagcc ccccgatgac cgctccctgt
     1501 atggagccta tgcccccgaa aaccgccgcg atgaaagcct tgaaacccgg tatgacgccc
     1561 atcaccggtt gtagctgggg gtacctcagg gcccacatta tccccgaagc ggccgccagc
     1621 gcggaaccca tgccgaaggt gagggcgatt atcctgttca ccggcactcc catgaggcgg
     1681 ctcgtctcga tatcccttga gatggccctc atgccgaggc cgggcttggt cttgtacacc
     1741 acataaagca gtcccatcat caggacgaag gatagcgccg ggacaacgag ggtcagcggc
     1801 aggatcctca ccttgccgac gatgataacc ttgaccagcc atgccgggcg ctgcacttcc
     1861 ctgggtatcg cggagaaaac gacaatagcg aagttctgca gaaagaagga aaccccgata
     1921 gcgcttatca gggccgagat cctgggggcg ttcctcaagg gtttgtaggc caccctgtca
     1981 acgattatcc cggcaaagga ggtcactacg atggccccga tgacggcgat gaaccaggga
     2041 aggcggaaaa gggtcactcc ccagaagaca aagtaagcgc ccaacatgaa gatctcacca
     2101 tgggcgaagt tgatcagcct caggataccg taaaccatgg tgtaacctat ggctataagg
     2161 gcgtagaggc tccccagggt gagtccgttt aaaaggtgct gaacgaacat agccaggctc
     2221 atctgtgctc ccccttgcga tcacgtaaaa ggcgggggag ggaacttttt cctcccccgc
     2281 gcaaaccgcg atggctctcc cgtagagagc ggcttggatc aagctcagtt tacagttcgg
     2341 gagtgatcgt tgctatatat tgcttcttgc catccttgat ctgcacgatg ccgattggct
     2401 tctcggcgtc gtgggtctcg ttgatggtgg tgagcccggt cacgccctcg aaatccttgg
     2461 tggccgcaag ggcggcggtg atcgcttcag gatcggcgct gccggccctc tcgatggcat
     2521 cgatgaggat catgtaggaa tcataaccga gagctgcgtt gacgttcggg tcatcggagc
     2581 gcttggccag ccaattgtcg gtaaaacgcc tggcgacggg attcatctcc ttcatcgagg
     2641 gatcgtaggg gaaggtcgtg tggacgaacc cctcgacggc atcgccaccg atctcgacga
     2701 tcttggggtt gtccatggcg tcgccgccca tgatctggaa gtccacgccc agttcctggg
     2761 cctgcttcat tatgatagcg ccctcggcga agtaagccgg aatgaacatg atgtcgggtt
     2821 ccttgctgat gatctcggtc aactgggcgg tgaagtcctg gtcgccggag ttgtaattca
     2881 gcttggctac gatctctccc ccgagtttaa ggaaggattt ctcgaagaag cccgccagcc
     2941 ctacagcata gtcgttggcc acatcggtca gcagggccgc ctttcttgcg ccgatcacct
     3001 tgtaggcgta ggtggcggct ccagcaccct ggagggggtc gatgaagcaa acgcggaaga
     3061 tgtacttctt gccctgggtg acgagggggt ttgtgcagga ggtcccgacc atcgggatgc
     3121 cggcgctttc ggccacttca ccgccggcca tggctaggga cgacccgtag gacccgatca
     3181 ctgccacggc attatccctt tcgataagcc gctttacggc attggcggac tccactttgt
     3241 cggacttgtt gtccacgacg ctgagctcca ccttcctgcc gagcacttcg ggtttctctt
     3301 catgggccat tttgacgccg tcaagttcca gctggcctcc gaaggcgttc tggccggtca
     3361 ggggaaggta aacacccacg tggatcacgc cgctctcaac cgccgcttgc tcttctttcg
     3421 gctcttccgc tggttcgggc gcgggtgctt ccgcggcggg ttccggtgcg ggggcctcgg
     3481 gtgccttctg cccctggatc atcatgaacc ctatgaccgc gatcaccacg atcacaccca
     3541 atacaagacc ttttttcact taagctcacc tccagatgta ttgtcgccgt acaaccgatt
     3601 ctacacgatt ccgtcttcca gggcaccagg acgcccctca caaaagggtc taccacctct
     3661 cttcccacaa aaggccgccc cggggggaca ggcggcgtca ctcccggcag cgggtacagg
     3721 cttaacgaaa acccttgcgc ttgacgcgat cattatactc tatcaaagga ataatgcatc
     3781 gagagagttg actttcaatt tctttttcac ggggttgcta tcggtggccg tttctctcgt
     3841 cccggagcct cgatatctcc tcgacgaaac tggagacatc ggtgaattcc cggtaaacag
     3901 aggcaaacct cacgtaagcc actttgtgga tgtccctcaa ttccgccatg gccagggacc
     3961 ccacggtcag actcgagact tccccctggc ctgactccct cagggcttcc tcgatcctgg
     4021 aaacggcttc ctcgatctgc tccagggata caggcagctt ttcacaggcc ttggcaaatc
     4081 ccttgagcag tttccccctg tcaaaagcct ccctttggcc gctcttcttg acgatccaca
     4141 gtacggggcg atcctcgaat ctttcatagg tggtgaagcg gtactggcac cccgggcact
     4201 cccttcgcct gcgaatgacc ctcccttcgc cggcggtcct ggtctcgagg accctggtct
     4261 ccatcaagcc gcacttggga caacgcatgg catggccccc ctgtttccag gtttttttca
     4321 ttttatcacg acgacccggg gtccctccgt caagcggggg ccccgccttg ggcggagccc
     4381 ccgcttcact ttgggctatc ttgggctaac tcgctctact gcttcatttc gttatccggg
     4441 gttctccacc atccctgtct ttttcttgga actcccccgt ggtcgtctcc cgctaccttc
     4501 ctctacgttt tcttgaagcg tcctctctct ttcttttctt ggcctcactg ggtttctcgt
     4561 aatgcttccg cctgcgcgcc tcacgcagaa tgcctccctt tgatacctct cgcttgaagc
     4621 gcttgagagc atcttcgagg gattcgttgt cgcgtcgaac gacctgtgtc atatgcgttc
     4681 ccccccttct ctctcactca gggctatccc ggcggccatc cgaaaacccg tccggcaagc
     4741 agatggacat ggagatggtt cacggtttgt cctgcgcccg ccccacagtt gaccacgagg
     4801 cgatacccac cctcacgaag gtccagctca cgggcgacct tgacggcagc gtccatgaca
     4861 gtggaccatg tcgagggatc ttcgatgtca tcggcggacc ccacgtgccg cttgggtatc
     4921 acgaggagat gcaccggcgc cttggggtgg atatcccgga tcaccaccac atcattatcg
     4981 ctatagagga tatccgcctt cccgcgccct tgcacgatcc tgcagaatgg acactcgtgg
     5041 tccaaaggat caactccctg ccgataccca tcggccccct ttgccggaag ggactcgatg
     5101 atacctggta gggaattata tcacaccaac ccgataaggg cgccttttac cgaatctttg
     5161 atcgtcaacg tagcgatctc gcccggccgg ggatccccgc gggtctcgac ccgcaggtaa
     5221 tgccgtgaga gaccgctgat ctttccctcc tggttcctct ccacaaggac ctccacttcc
     5281 ttgccgaccc accgccttga atagagcccc agcaattcgt agcccgtcat cagggcacgg
     5341 tccatcctgg aacggatctc gtcgggggga atgggttttt caaagttgga agccggggta
     5401 ccctctcggg ctgaaaaggg gaaagcgtgg agtttcccca ccctcgtcct ctcgagcatc
     5461 t
//
LOCUS       DAOSRG010000058         5285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14242_length_5285_cov_9.437667, whole genome shotgun sequence.
ACCESSION   DAOSRG010000058 DAOSRG010000000
VERSION     DAOSRG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14242_length_5285_cov_9.437667"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLM30_03300"
     CDS             <1..455
                     /locus_tag="PLM30_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOR54230.1"
                     /translation="ERAAAVLEGRDYEWAVFHAREASVGSVSDENCHPFVIEGRHHLV
                     LAANGSESAMAALGRVIGDRTDSEFIAKMIVDMDLPFPQVLSCFNSNFVGLYDGRPFA
                     KKGDRQLLKYQTERDIVFASDLPFGLTGITRPGHNYCWFDWEESVLPG"
     gene            473..1507
                     /locus_tag="PLM30_03305"
     CDS             473..1507
                     /locus_tag="PLM30_03305"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOR54231.1"
                     /translation="MGKITVYGAGSWGTAAADLLARGGHQATLWCRRPEQARAINATG
                     RNPDYLKCTDLSPALSVTADLEEAAVSSQFHVTAVPTQSVREFLSGVASYWPRDVRVC
                     NLAKGIEIASGDRISQVVAGTLPGSRYSILSGPSHAEEVAGGKPAAVVAASSIPGEAE
                     LWQGIFSGKLFRVYTSDDVVGVETGGAVKNVIAIAAGIAASREMGHNALAALVCRGLA
                     EVMRLGAKLGAHPLTLAGLAGVGDLMVTCFSGLSRNYRLGLAVGGGMTLTQALNSLGQ
                     VAEGAYTVDALVKNAREIDIELPLTEAVHRILYEGVSPESMVGSLFGRELKPELPPEM
                     YWGRGTNSGD"
     gene            complement(1504..1998)
                     /locus_tag="PLM30_03310"
     CDS             complement(1504..1998)
                     /locus_tag="PLM30_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR54232.1"
                     /translation="MVLFLAFAAFVALSSSLHFEPGTRCGVDFLAFARTMAGLLPPTF
                     LLVGLFYAWVKRETVEKHMGRESGVQGYFWAIVLAGTIVGGLFVALPLCLVLQKKGAS
                     MKVILTFLNASMICRIPMTVFEASFLGVGFTIVRFAVSIPLLILIAWGMSSWLGGGFR
                     ISEP"
     gene            complement(2028..2510)
                     /locus_tag="PLM30_03315"
     CDS             complement(2028..2510)
                     /locus_tag="PLM30_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54233.1"
                     /translation="MVVAWVLMAALFSVSWFVNRERTLEAARLAWSLFREVLPMFVIV
                     LVFTSLSLGLISEEVLLELLVGRGLLVGSVTGAVAGSIAAIPSSVAFPLSAILLDKNV
                     PYTVIAAFTTTLMMVGVVTFPLEREYLGFRAALVRNLAGLAMALVVSVAIGCFFGELP
                     "
     gene            2689..>3714
                     /locus_tag="PLM30_03320"
     CDS             2689..>3714
                     /locus_tag="PLM30_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54234.1"
                     /translation="MVRSLQEAILAALSSASRLSDRGHEAVVVGGAVRDLLLGRGSAE
                     ADLATSAPWDQVLELWPGAPVVGHPPAATVITRSGGHRVDISCFQGGSLEEDLGRRDL
                     TINAMAVTADGTIVDPWGGTADLAKGLLRFTGDPRERLASDPLRAVRLARFAAELPSF
                     RVDPASLEACRGFASRVASMPPPRIGREALKALDGDLPTFLDSLERQGIIASVLPSWG
                     EILSDRKNAALERVRRACSLTYDRAVRAACLLADAGPGVGAMVSAWGWPKGLAREAVN
                     LVRYRSLALRGGNAGDWASLFRSQGAPWLGKLLLFGYIDGLGSDPLTVEPAMYMLRLQ
                     AFGPRVTG"
     assembly_gap    3715..3814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3815..4889
                     /locus_tag="PLM30_03325"
     CDS             <3815..4889
                     /locus_tag="PLM30_03325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR54235.1"
                     /translation="EFSKQQRRRKIATCFVLSGWYATLFITDNLFGYHPAVQVDGALA
                     LVFLASAFGTAFMTSWKKRSLLGLSAIAGLLLISSTPLLFTVLGLPLSILLLRHVMTL
                     FFCVMVAPYFLKPRGFAFFLVLLYNALCFYYPILRTRELDETMAYVIVTYSFSLLFAY
                     FFLDILRMNLDHVSSLIAAKRDAEDRALRDDLTGVYNRRYCDNLLRVLIENETEKALL
                     FIDLDDYQNVYQAHGHLVGDAYLKRSAEAISESIRSGDVVSRFSGNQFVVVLDSNDLT
                     LAETVARRIIKAVGNVIPEFEGISVGVTKIIPGITQQAVLVNADTALYRSKRSHLVGI
                     SIVDPANPRIEPELWPETDMTPQ"
     gene            5053..>5285
                     /locus_tag="PLM30_03330"
     CDS             5053..>5285
                     /locus_tag="PLM30_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR54236.1"
                     /translation="MKGTGRSAMGDGALEVILSRRSIRKYTDRAIEEGSLELLLRAAM
                     SAPSAHDQRPWEFVILDDRETLRAITLFHPYSR"
BASE COUNT          988 a         1690 c         1656 g          851 t
ORIGIN      
        1 ccgagcgcgc cgcggccgta ctggaggggc gcgactacga gtgggccgtc ttccacgcca
       61 gggaggccag cgtcgggtcc gtcagcgatg agaactgcca ccccttcgtc atcgaggggc
      121 gacatcacct ggtcctcgcc gcgaacggga gcgagagcgc catggccgct ttgggacgcg
      181 tcataggcga caggaccgac agtgagttca tagcgaagat gatcgtggat atggacctcc
      241 ccttccctca ggtcctgtcc tgcttcaact caaacttcgt cggcctttac gacggccggc
      301 ccttcgcgaa aaagggcgac cggcaactgc tcaagtacca gacggaacgg gacatcgtct
      361 tcgccagcga cctccccttc gggctgacgg gcattaccag gccgggacat aactactgct
      421 ggttcgactg ggaagagtcc gtgctccccg gctgagcccg gaaggaggcg ccatgggcaa
      481 gatcaccgtc tacggagcag gaagctgggg caccgccgcc gccgacctcc tcgcccgggg
      541 ggggcaccag gccaccctct ggtgcaggag accggaacag gcgcgggcca ttaacgccac
      601 ggggaggaac cccgattacc tcaagtgtac cgacctcagc cccgccctct cggtcacggc
      661 ggacctcgaa gaggctgccg tctcctcgca gtttcacgtc accgccgtgc ccacccagtc
      721 ggtcagggag ttcctttcgg gcgtggcgag ttactggcct cgtgacgtga gggtgtgcaa
      781 cctggcgaag gggatagaga tcgcgtcggg cgacaggata agccaggtcg tcgccgggac
      841 cctccccggg tccaggtact ccatcctgtc gggaccgagc cacgctgaag aggtcgccgg
      901 cggcaagccc gccgccgtgg tggccgcttc ctccatcccc ggcgaggcgg agctctggca
      961 ggggatcttc tcggggaaac tattccgggt ctacaccagc gacgacgtcg tcggcgtaga
     1021 gacagggggg gcggtcaaga atgtcatcgc catcgccgcc gggatagccg cctccaggga
     1081 gatggggcac aacgccctgg cagcgctggt gtgcaggggc ctggcggagg tgatgaggct
     1141 cggcgcgaag ttgggcgcgc acccccttac actggccggg cttgccgggg tgggcgacct
     1201 gatggttacc tgcttcagcg gcctctcgcg aaactacagg ctgggcctgg ccgtcggcgg
     1261 ggggatgacc ctgacgcagg ccttgaattc cctgggccag gtggccgagg gcgcttatac
     1321 cgtggacgcc ctggtgaaga acgcccggga gatcgacatc gaactacccc tcaccgaggc
     1381 ggtgcaccgc atcctatacg agggggtctc acccgagagc atggtgggat cccttttcgg
     1441 ccgtgaactg aaaccggaac tgccgccgga gatgtactgg ggaagaggga cgaacagcgg
     1501 cgactagggt tcggagatcc tgaaaccccc gccgagccag gaagacatgc cccaggctat
     1561 caggatcagg agcggtatcg agacggcgaa gcggacgatg gtaaaaccta cccccaggaa
     1621 agaagcctcg aagaccgtca tggggatcct gcagatcatg gaggcgttga gaaaggtcag
     1681 gatcaccttc atggaggccc ccttcttctg aaggaccagg cagagaggca gggcgacgaa
     1741 aagaccgccc actatggtcc ccgccagcac gatggcccag aagtaaccct gaaccccgga
     1801 ctcacgcccc atgtgttttt ccacggtctc ccgcttcacc caggcgtaga acagccccac
     1861 caggagaaag gtcggcggca aaaggcccgc catcgttcgg gcgaaggcca ggaagtcgac
     1921 gccgcacctg gttccgggct caaaatgaag cgacgaggag agcgcgacaa aggccgcgaa
     1981 agccaggaac agaaccaccc agaaaggcaa ggacgacctc ttcccgctca tggcaactct
     2041 ccgaagaagc agccgatggc cacggaaacg acgagggcca tcgccagccc ggcgaggttc
     2101 ctgaccaggg cggccctgaa accgaggtac tccctctcca ggggaaaggt caccacgccg
     2161 accatcatga gggtcgtggt gaaggccgct atcaccgtgt agggtacgtt tttatcaagg
     2221 aggatggctg acagggggaa ggccacggaa ctgggtatgg cggcgatgga accggccacc
     2281 gccccggtga cgctcccgac gaggagcccc ctgccgacga gcagttcgag aaggacctct
     2341 tcgctgatga gccccaggct gagggatgtg aacaccagga cgatcacgaa catggggagg
     2401 acttccctga aaagggacca ggccagccgg gctgcttcca gggttctctc ccggttcacg
     2461 aaccacgata ccgaaaaaag agccgccatg agcacccacg ccaccaccat gccttccgct
     2521 cccccctttg accccggcct cggcgaaacc gccgcgtcat tcccgctccg cccgggccac
     2581 cagtataccg aaaaaactcc tccaggggac gacccggcac ggaatggacc ggagggggca
     2641 tcccagggtg atatcgatgc cgctgcgaag tgatacaatg cggtaaaggt ggtgagatcc
     2701 ctgcaggaag cgatcctggc cgccctcagc tccgcgtcga ggctctccga taggggacat
     2761 gaggccgtcg tggtgggcgg ggctgtccgc gacctcttgc taggccgggg atcggcggag
     2821 gccgacctgg cgacttcggc cccatgggac caggtcctgg aactgtggcc cggggcccct
     2881 gtcgtggggc accccccggc cgccacggtc ataacccgct ccggcggaca ccgggtggat
     2941 atctcctgtt tccagggggg aagtctcgag gaggacctgg ggcggaggga cctcaccatc
     3001 aacgccatgg cggttaccgc cgacggcacg atcgtggacc cctggggggg gacggccgac
     3061 ctggcgaagg ggctgctgcg cttcaccggc gacccccgtg agaggctggc ttccgacccc
     3121 ctgagggccg tgaggctcgc ccggttcgcc gccgagcttc ccagcttcag ggtcgacccc
     3181 gcttccctcg aagcctgccg cgggttcgcc tcccgggtag cctctatgcc gccgccccgc
     3241 atcggaaggg aggctctgaa ggccctggat ggggacctgc ccaccttcct ggatagcctc
     3301 gagcggcagg gcatcatcgc ttcggtcctg ccctcttggg gagagatact ctccgacagg
     3361 aagaacgccg cgctggagcg ggtacgcagg gcgtgctcct taacgtacga ccgggctgtc
     3421 agggcggcat gcctgctggc cgacgccggc ccgggcgtcg gagccatggt ctccgcctgg
     3481 ggctggccga aagggctggc cagggaggcg gtgaacctcg tgaggtaccg atctctcgcc
     3541 ctccggggcg gcaacgccgg cgactgggcc tcccttttca ggtcccaggg agcgccctgg
     3601 cttggaaaac tcctcctgtt cgggtatatt gacgggctgg gaagcgatcc cttgaccgtg
     3661 gaaccggcga tgtacatgct gcgcctccag gccttcgggc ccagggtcac cggcnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaatt cagcaagcag caacggaggc
     3841 ggaagatagc gacctgcttc gtgctttcgg gctggtacgc gacattgttc atcaccgaca
     3901 acctcttcgg gtaccacccg gcggtgcagg tcgacggggc tttggccctg gtctttttag
     3961 ccagcgcctt cggcacggcc ttcatgacct cctggaagaa gcgttccttg ctggggttgt
     4021 cggccatagc cgggctgctc ctcatttcct ccacgccgct ccttttcacc gtgctgggcc
     4081 ttcccttaag catactgctc ctgcgtcatg tcatgaccct cttcttctgc gtcatggttg
     4141 ccccctattt tctgaagccc agaggttttg ccttcttcct ggtcctcctt tacaacgccc
     4201 tttgcttcta ctaccccatc ctccgcacga gggaactgga cgagaccatg gcctacgtga
     4261 tagtgaccta ctccttctcc ctcctcttcg cctacttctt cctggacatc ctgaggatga
     4321 acctggacca tgtctcctcc ctcatcgccg cgaaaaggga cgccgaggac agggcgctca
     4381 gggatgacct cacgggggtc tacaacagga gatattgcga caacctcctc cgggtgctca
     4441 tagagaacga gaccgaaaag gccctcctct tcatcgacct cgacgactac cagaacgtgt
     4501 accaggccca tggccacctg gtaggggacg cctatctcaa gagatcggcc gaggccatat
     4561 cggaatcgat caggtccggc gacgtggtct cgcgtttcag cgggaaccag ttcgtggtgg
     4621 tcctcgacag caatgacctg accctggccg agacggtggc caggaggatc atcaaggccg
     4681 tcgggaacgt gatccccgaa ttcgagggca tctccgtggg tgtgaccaag atcattccgg
     4741 ggataaccca gcaggccgtg ctggtcaacg ccgacacggc cctctaccgg tcgaagcgct
     4801 cgcacctggt gggcatctcc atcgtggacc ccgccaaccc ccggatcgag ccggaactct
     4861 ggcctgaaac ggacatgacc ccccagtaaa gatgatgaga ggggccccag gagggcccct
     4921 cttcagtcag ggcaggggtg ccctccccga ggcgaaaagg ccccggagga gtttccccac
     4981 ccacagtatg gccgaggcca cggccttgaa gaggattcac acggccttca cctgcctcgt
     5041 gccaacgata ccatgaaagg aaccgggagg tccgccatgg gtgacggggc tcttgaagtc
     5101 atcctctcca ggagaagcat caggaaatac accgacaggg ccatcgaaga ggggtccctc
     5161 gagctgctcc tgcgcgccgc gatgagcgct cccagcgccc atgaccagcg gccctgggag
     5221 ttcgtcatcc tcgacgacag ggagaccctg cgggcgatca cgctgttcca cccctattcc
     5281 aggat
//
LOCUS       DAOSRG010000059         5251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14370_length_5251_cov_9.809469, whole genome shotgun sequence.
ACCESSION   DAOSRG010000059 DAOSRG010000000
VERSION     DAOSRG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_5251_cov_9.809469"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLM30_03335"
     CDS             <1..740
                     /locus_tag="PLM30_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54237.1"
                     /translation="ITILVTAVIMGAVITVLSLFFSNFQFVSSGVSARQRAEMVLSIL
                     AKPTQHAGLGMPVTSGDFQAAFDQVYTPEITPQDYPSALHITGSGELHLAFGEPTGLS
                     VLLPEDIQAGSGATIQVQLNGAPEGIVPGDWVVFPTGVSPLVVRSVSGNDVTLENRGG
                     PTGISRLDELHKVKFFRAYCEDGLFHALDPRPGRGGGVTVEGVEGLSVNFDEETRVIS
                     VTITGRGKEGNWEDFEDIEASWRVKNL"
     gene            737..1357
                     /locus_tag="PLM30_03340"
     CDS             737..1357
                     /locus_tag="PLM30_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54238.1"
                     /translation="MRTGPKRTGAVLPLTLVVLLVGIAFSAVVLVMVENYFSTSTNLV
                     REIDVTGEAEGGLEEGKQWLDGYIWTHDRLPRFSPDDPEDLTGRVNVTGDPSVLRVHH
                     IPPAPAAGNPRVMVEVEVFDLNYDVGTIALDPGEIFPPRLDATMIEYMGSWHMSSSYA
                     ASNRGEGSVGAGFGVDRYGVYLVRSRAVLGEKEKSLSEALIRVRGK"
     gene            1431..5072
                     /locus_tag="PLM30_03345"
     CDS             1431..5072
                     /locus_tag="PLM30_03345"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOR54239.1"
                     /translation="MAAAVLIATLLSAGSAQAAFTITKTVDISDPNPGDYIVFTINLG
                     NTPRSELSGATLTDNMPSFEEVTYTYNRVGSGQPSTVPVGPLPWTGIFTGFTGSSNPR
                     NYTVREIKIKARVPEGRAGQDIPNEARISKQGIATKTSSVTVHVRNVVPAVAPQVFTP
                     YAKPAPALYENRVEPNVLLLLDTSGSMTYESDSEDTTWGDGSKPVSFGFSSRQYYYGK
                     DTDSSNNDPGNPSNYHPNLKYVPQAEVDLIDSDQGSQAKTWLGGEHPNDDPPSGYSRY
                     KYPNDSRLYKMKLVLNKILSDPELTSGLRIALATYHQKESAGGTDADWYQFTLQSSRY
                     PYNYSNETQKLHYAKEGDPKALLRVPFGSADDPSHLSDILKWFDGVEESGNPELRAAG
                     WTPLAASIYGTQVTQDSAVKFFKANDVVQWRCQYNWLIVLTDGADTEKGNPVESVRKL
                     YNENITASEDTQRGLPVRTLVIGLIDPEEMTELAGTLENMADLGWDGQVGEADPADPS
                     PGAFFSTDIESLIYTFRRIFEIIQASRTTSGAPMVSPARTEAGDSSIYVASFLPQNSA
                     QWKGHLFQYTVGEDGALDEDANWDASSTLQELEPGGRKIFTVDWAGMPSPKAQGLGGG
                     TNLVNFDGSASLMDEVTGSGEYSLSSDFLALFISWVRGFDIWNENPASAGGHRYMFAD
                     VYHGGVTEVGPPRANYPSQAYADFAASHEERDKTLYLQSNAGLLQAIDPEKDGEEMWA
                     FIPPNVLGNGRLLGLKGQFSNDGKVEVFNRTPSIPRYLLDGPLVAEDVLLQGEWRTIL
                     VGCLGYGGQGLYALDVTEPGEPVFLWAVENNIVTPDGSSALGADSRKIHYWKKSRSLQ
                     VDYTIHPHAEVGEELDYRGLYRTLSTPIVANTVGVVDGGQGLWEDRWVAVMGNGHPGN
                     FGGNPGGGAVYMIDVQSGEVLRKLTARDSGGANVLKPVCSPVTGFGRSMATRLLDHLY
                     AGDIGGNVYSWESGRDNWGTALKIFQAPAGDAGITYRMDVGMLGGQPWLFYQTGDIEN
                     LNFSTSGYRLYALNTSRASEQSPLTIDDLEGLTPTQGTASNTNGWYLDFRATPAEIPS
                     TPVTFYNGYLLFATFSGNFSDPCAIGSSRFYAVNALTGGGAWGGGNKFVQLEGLQVAG
                     ITVFGDKVYLGVVGSASSGDLKSALGDSARLTGNLLSFDLPEEIPSSGDAGAGADEGP
                     IFWREWRTK"
     gene            5057..>5251
                     /locus_tag="PLM30_03350"
     CDS             5057..>5251
                     /locus_tag="PLM30_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54240.1"
                     /translation="MEDKMMRSPAYPGARVLLVMIALALYLFLSGAAAPALAQDAGPP
                     PTGEEEVIYENKVGAVLENLT"
BASE COUNT         1036 a         1667 c         1627 g          921 t
ORIGIN      
        1 tgatcaccat cctggtgacg gcggtcatca tgggcgcggt gatcaccgtg ctgtcgcttt
       61 ttttctccaa cttccagttc gtctcttccg gcgtctcggc tcgccagagg gcggaaatgg
      121 tcctttccat cctggcgaag cccacccaac acgcggggct cgggatgccc gtgacctcgg
      181 gtgacttcca ggcggccttc gaccaggtct acacacccga gatcaccccc caggactacc
      241 cctccgctct tcacatcaca gggtcggggg aactccacct ggccttcggg gaacccacgg
      301 ggctctccgt cctcctccct gaggacatcc aggcaggctc cggcgccacg atccaggtcc
      361 agttgaacgg agcacctgaa ggtatcgtcc ctggtgactg ggtcgtcttc cccaccggcg
      421 tctcacctct ggtcgtaagg tccgtctccg gcaacgacgt gaccctcgag aaccgcggag
      481 gcccgacggg catctcccgg ctggacgaac tgcacaaggt caagttcttc agggcttatt
      541 gcgaggacgg ccttttccac gcccttgacc cgaggccggg gaggggcggc ggcgttaccg
      601 tcgaaggcgt cgagggactt tcggtgaact ttgacgaaga aacgagggtg ataagtgtca
      661 ccatcaccgg ccgcggcaag gaggggaact gggaggactt cgaggatatc gaggccagtt
      721 ggagggtgaa gaacctatga gaacaggtcc taagagaacg ggagccgtac tgcccctcac
      781 cctggtcgtc ctcctcgtgg ggatagcctt ttctgctgta gtgcttgtga tggtggagaa
      841 ttacttttcc acttcgacga acctggtcag ggagatcgac gtcaccggcg aggccgaagg
      901 agggctcgag gagggaaagc aatggctgga cggctatatc tggacccacg acaggctgcc
      961 gaggttttcc cccgatgacc ccgaggacct gacgggaagg gtgaatgtca cgggagaccc
     1021 ctcggtcctc agggtgcacc atattccccc cgccccggcg gcggggaacc ccagggtcat
     1081 ggtggaggtg gaggtcttcg acctgaacta cgacgtcggg accatcgccc tggaccccgg
     1141 ggagatcttc cccccccggc ttgacgcgac gatgatcgag tacatggggt catggcacat
     1201 gagttcctcc tacgccgcct ccaaccgcgg ggagggcagc gtcggcgccg gcttcggggt
     1261 ggaccggtac ggcgtctacc tcgtcaggag cagggccgtc ctcggggaga aggaaaagtc
     1321 cctgagcgag gccctcatcc gcgtccgggg caagtgagcc cgaaaggcag gtgaccggta
     1381 tgaagggaag aggtaaggaa agagtccggc gactccttgg aggcgccgga ttggccgccg
     1441 ccgtcctcat cgccacgctg ctttccgccg ggtcggccca ggcggctttc accataacca
     1501 agacggtgga catctccgac cccaacccag gggactatat tgttttcacg atcaacctgg
     1561 gaaacactcc ccggagcgaa ctatcaggtg ccacccttac ggataacatg ccatcctttg
     1621 aagaggtcac ttacacttat aaccgtgtcg ggtcggggca accatccact gtacctgtgg
     1681 ggcctctacc ctggacgggc attttcaccg gctttacggg atcctctaat cccaggaatt
     1741 acaccgtgag ggagatcaag atcaaggcac gggtaccgga ggggcgggcc ggccaggata
     1801 tccccaacga ggcaaggata tctaaacaag ggatcgccac gaaaacctcg tcggtgacgg
     1861 tccacgtccg aaacgtcgtt cccgccgtgg ctccccaggt attcaccccc tacgccaagc
     1921 ccgcccccgc cctttacgag aacagggtcg agcccaacgt gctcctcctc ctcgacacga
     1981 gcgggtccat gacctacgaa tccgattcgg aagatacgac ctggggcgac ggctccaagc
     2041 cggtgagttt tggcttttcg agccgccagt actactatgg caaggacacg gacagcagca
     2101 acaacgaccc cgggaaccct tcgaactatc accccaacct caagtacgtc ccccaggccg
     2161 aggtggacct gatcgactcc gaccagggca gccaggcgaa gacatggctg gggggcgagc
     2221 accccaacga cgaccccccg tcgggttatt cccgttacaa gtatcccaac gacagccgcc
     2281 tctacaagat gaagctggtc ctcaacaaga tattgtccga cccggaactg acctcgggcc
     2341 tgaggatcgc cctggccacc tatcaccaga aggagtcggc cggaggcact gacgccgatt
     2401 ggtaccagtt tacgcttcaa agtagccgat atccctataa ctattccaac gagacccaaa
     2461 aattacacta cgccaaggag ggcgacccca aggccctttt gagagtgccc ttcggctccg
     2521 ccgacgatcc ctcccatctt tcggacatcc tgaaatggtt cgacggcgtg gaggaaagcg
     2581 gcaaccccga actccgagcg gcggggtgga ctcccctcgc ggcctcgatc tacggtaccc
     2641 aggttaccca ggattcggcg gtgaagttct tcaaggccaa tgacgtggtc cagtggcggt
     2701 gccagtacaa ctggctcatt gttctcaccg acggggccga cacggaaaaa ggtaaccccg
     2761 tggaatctgt caggaaattg tacaacgaga acatcacggc ctctgaggat acccagcgcg
     2821 gccttccagt gaggaccctg gtgataggcc tcatcgatcc tgaggagatg acggagctgg
     2881 ccggcaccct ggaaaatatg gccgaccttg gctgggacgg ccaggtcggc gaggccgacc
     2941 cggcggaccc gagtcccggg gccttcttct cgaccgatat cgaaagcctg atctatacct
     3001 tccggagaat tttcgagatc atccaggctt ccaggacgac cagcggggcc cccatggtca
     3061 gccccgcccg gaccgaggcc ggcgactcgt ccatctacgt ggcctccttc ctcccccaga
     3121 attcggccca gtggaaggga cacctcttcc agtacaccgt gggcgaggat ggcgccctcg
     3181 acgaggatgc caactgggat gcctcgagca ccctccagga gcttgagcct ggcggaagga
     3241 agatattcac cgtcgactgg gccggcatgc cttctcccaa ggctcagggc ctgggcggcg
     3301 ggaccaacct cgtgaacttt gacggctcag cttcgttgat ggacgaggtg accggttccg
     3361 gggagtactc cctttcgtcg gatttcctcg cccttttcat cagctgggtc aggggtttcg
     3421 atatatggaa cgagaacccg gcgtccgccg gggggcaccg gtacatgttc gccgatgtct
     3481 accacggggg cgtgacggag gtggggcctc ccagggccaa ctacccgtcc caggcctatg
     3541 ccgatttcgc cgcgtcccac gaagagaggg acaagaccct ctacctgcag agcaacgcgg
     3601 ggcttctgca ggcgatcgat cccgaaaagg acggagaaga gatgtgggcc ttcatccccc
     3661 ccaacgtact gggcaacggc aggctcctcg gcctcaaggg acaattctcc aacgacggca
     3721 aggtggaagt gttcaacagg acgccctcca tccctcgata cctcctcgac gggcccctcg
     3781 tggccgagga cgtcctcctg cagggcgaat ggaggaccat cctggtaggc tgcctgggat
     3841 acggtgggca gggcctgtat gccctcgacg tgacggagcc gggagagccc gtctttttat
     3901 gggccgtgga gaacaacatc gtcacgcccg acggctcctc ggccctgggt gccgattcga
     3961 ggaagatcca ctactggaag aagagccgaa gcctccaggt ggactacacc atccaccccc
     4021 acgccgaggt cggggaggag cttgactacc gcggccttta caggaccctg agcaccccga
     4081 tcgtggccaa caccgtcggc gtggtggacg gggggcaagg cctttgggag gaccgttggg
     4141 tcgcggtcat gggcaacggc catcccggga atttcggcgg taatcccggc gggggcgccg
     4201 tctacatgat cgacgtccaa agcggggagg ttctgaggaa actgaccgcc cgggactcag
     4261 gcggtgccaa cgtgctcaag cccgtttgct ccccggtgac cggcttcgga cggagcatgg
     4321 ccaccaggct cctggaccac ctttacgccg gggacatagg cgggaacgtc tactcctggg
     4381 agtccggccg tgacaactgg ggcaccgccc tcaagatctt ccaggccccg gcgggtgacg
     4441 ccggcatcac ctacaggatg gacgtgggca tgctgggagg acagccctgg ctgttctacc
     4501 agacggggga tatcgagaac ctgaacttct ccacctccgg ctacaggctc tacgccctca
     4561 acacctccag ggcgtcggaa cagagccccc tgaccatcga tgacctggag gggttgaccc
     4621 caacccaggg gacggcgtcg aacaccaacg ggtggtacct ggatttcagg gccaccccgg
     4681 cggagatacc ctcgacgccg gtgacctttt acaacggcta cctcctcttc gccaccttct
     4741 cggggaactt cagcgatccc tgcgcgatcg gaagttccag gttctacgcg gtgaacgctc
     4801 tgaccggcgg cggtgcctgg gggggcggga acaagttcgt ccagctggag gggctccagg
     4861 tggcggggat caccgtcttc ggcgacaagg tctacctggg cgtcgtgggt tcggcctcat
     4921 cgggggacct caagagcgcc ctcggtgaca gcgcccggtt gacggggaac ctgctctcct
     4981 tcgaccttcc cgaggagata ccttccagcg gggatgccgg cgccggggcc gacgaggggc
     5041 ccattttctg gagggagtgg aggacaaaat gatgagatct cccgcttatc ccggggcaag
     5101 ggtcctgctg gtcatgatcg ccttggccct ttatctcttc ctgtcggggg ccgcggcccc
     5161 agccctggcg caggatgcgg gtcccccgcc aaccggggaa gaagaggtga tctacgagaa
     5221 caaagtgggt gctgtcctgg aaaacctcac g
//
LOCUS       DAOSRG010000060         5124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14819_length_5124_cov_11.382127, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000060 DAOSRG010000000
VERSION     DAOSRG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14819_length_5124_cov_11.382127"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..390)
                     /locus_tag="PLM30_03355"
     CDS             complement(64..390)
                     /locus_tag="PLM30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOR54241.1"
                     /translation="MSDGKNLLMAAVGDFESVLPFQAVGVSPFLPENGLRELLLGLAK
                     EHYAVVFLVDSLYRDNQEMVEELNENYAMSIIPVPGIRGSTGIGIKTIRESVERAVGM
                     DIFSVK"
     gene            complement(383..1393)
                     /locus_tag="PLM30_03360"
     CDS             complement(383..1393)
                     /locus_tag="PLM30_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOR54242.1"
                     /translation="MADPADYGYAVSRLRAMENRLLDQGVYQRLLDSEDLPSALKVMS
                     ETAYGKWFLEQQNEDTFEPAIESELKHVYTETEQFVPDPCLYRIFRLPYDIHNIKVLL
                     KGIFKQKQGGQRRLDLLTGLGNIPVDDLVMAIESEDYRLMPFGFHRTVPESVSIWEQA
                     HDILQVERILDQALFSLQLKMADEVAFEGVKLFARSRVDTENIRNVARLKRMGFDSSQ
                     AAPFLHNGGSVPVEKIQSLINEPYEGWERILSFADVSRTLSGAQDHADLDSLIVDLEK
                     SIDDFLSWVLAKYKYSPFAPENVLHYLWMKETEAKNVRILLIGIGNGAERTVLRRLLR
                     NV"
     gene            complement(1407..1991)
                     /locus_tag="PLM30_03365"
     CDS             complement(1407..1991)
                     /locus_tag="PLM30_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOR54243.1"
                     /translation="MSLADIKTKIEADARNEAEGILGQFRAQADEILVTAKRESEMLD
                     KKLQERINHEEGEVRKRKTIVADLEVRKLMLGAKRELVDLVYEKAMAILSSLPRDRYA
                     GFMTALLGSSEVSGDEEVFLCKGEKVLDQKWLDEVNAQRQYNLTLSQDRVQGSGGFVL
                     RKGRIDINCTWEVLLGRVKEDLEAEVVDRLFPSR"
     gene            complement(2020..2496)
                     /locus_tag="PLM30_03370"
     CDS             complement(2020..2496)
                     /locus_tag="PLM30_03370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOR54244.1"
                     /translation="MDQLGIMLAVLGAALAAGFSGSGSAIGVGIAGESGAGIMTEDPG
                     KFGLVLLLQALPGTQGIYGLLTAFFAILKVGLLGGAVVSVNVWQGLGVLFACLPIAIA
                     GYYSAIAQGKTSAASIQMIAKRPEETGKAVILPAMVETYAVLALLMSIILLNSIQI"
     gene            complement(2553..4544)
                     /locus_tag="PLM30_03375"
     CDS             complement(2553..4544)
                     /locus_tag="PLM30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOR54245.1"
                     /translation="MDTTIAALQGLGCCEIIPPDGESRPEGGLGSTPPERIKPGRSSE
                     LETCISDCRFLLRFLEPLYAEKGGTLERLLGPKPPVTLTELASLRARTDLSALSSKAR
                     NLERSISEMRSETGQIANQLHLLEPIKELGLPLWVITRGTEKVMGVMGSIPTGRKASL
                     AEHFSSVVNGEGELFLSSPAAKETEAMAVLIFPRVFEQEVMESAARAGFSRIDCPPGL
                     EDKPAKEIERLAKRRGELAEEIASAEDEASRMATEVVPVVRNLSDYLAVLAARNEAGD
                     SGVSSEKVSVLWFWIPAGKMKDVEKALEPCKGLVDIYSEDPSPEDNPPIILENPRWAI
                     PFSPLTKLYGMPSYSGIDPTVLMAPFFFLFFGMCLGDGGYGLVLGAFLLYALLRYRLS
                     GDTKNFFVLLLLGSISTVLVGALTGSWLGDMIDVFGIFAPIKSLKDSLVLIKPMDDPI
                     TFLAIALGLGVVQIFFGILIAFWDAFRKGDMMGAFADQGGWLALLVGFLLWGASSLGV
                     GGGTTLFAGKALAVVGALTLVATQGRDKPGVIRKVVSGVLSLYNVTSYLGDVLSYSRL
                     LALGLATSAIAMIGNTLTILVSGIPYVGWVLAFLIFFGGHLFSVVVNVLGSFIHSLRL
                     QYVEFFSKFYSAGGRAFNPLRVATKFTRITGQGVEGPGF"
     gene            complement(4576..4959)
                     /locus_tag="PLM30_03380"
     CDS             complement(4576..4959)
                     /locus_tag="PLM30_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HOR54246.1"
                     /translation="MLKNFLIQYNACWEVIGVPKNIAEEITEAEVKARQIIQDARAEG
                     ARVLAQARAEAENLAKGSRQRYHRSLRDATLKLEEEADRESEKTIEKGRKEAEGLVQS
                     NKGKVDAVADWVAEEVMSRYGSDEG"
BASE COUNT         1153 a         1474 c         1297 g         1200 t
ORIGIN      
        1 tacgatcttc tctttgttgg ccaataatat cccctccgag tacactcgtg tcttccgtta
       61 cggctacttc acggaaaaaa tatccatccc gaccgctctt tcaacgcttt cgcggatcgt
      121 ttttatccct atccccgtgg aacccctgat gcccgggacg ggaatgatac tcatggcgta
      181 gttctcgttc aattcctcca ccatctcctg gttgtccctg taaagggagt caacgaggaa
      241 taccacggcg taatgttcct ttgccagccc caggagaagt tccctgagcc cgttctccgg
      301 caaaaaggga ctcacgccga cagcctggaa agggagtacg ctttcaaaat cacccacagc
      361 ggccattaaa aggttctttc cgtcagacat ttcttaaaag cctcctgagg accgtgcgtt
      421 ccgccccgtt ccctatgccg atgagaagga tcctgacgtt cttggcttcc gtttctttca
      481 tccagaggta gtgaaggaca ttctccggcg cgaaggggct gtatttgtac ttcgccagga
      541 cccaagacag gaagtcatct atggattttt cgaggtccac gatgagggaa tccagatccg
      601 cgtggtcctg tgccccggaa agtgtcctgg agacatcggc gaaggagagt attctttccc
      661 atccctcata gggttcattg ataagggact ggatcttttc caccggtacg gatccgccgt
      721 tatgaaggaa cggagcggcc tgggacgagt cgaaacccat ccttttgagc ctggcaacgt
      781 tcctgatatt ctccgtatcg accctggacc gcgcgaacag tttcactcct tcgaaagcca
      841 cttcgtctgc catcttcagc tggagtgaga agagcgcctg gtcgagaatg cgctctacct
      901 ggaggatgtc gtgagcctgt tcccagatag aaacggattc cggcacggtc ctgtgaaatc
      961 cgaagggcat gaggcggtag tcttcgctct cgatggccat caccaggtca tccacgggga
     1021 tgttcccaag gccggtcaga aggtccaacc gcctctgccc cccctgtttc tgtttgaaaa
     1081 tacctttcag gaggaccttg atattatgga tgtcataggg aagcctgaag atcctgtaca
     1141 ggcaagggtc gggaacaaat tgttccgttt cggtatagac gtgcttgagt tccgattcta
     1201 tggcgggctc aaaggtatct tcgttctgct gctcaaggaa ccatttcccg taggccgttt
     1261 cggacatcac ctttagggcc gaaggcaggt cttcgctgtc caggagccgt tgatacactc
     1321 cctggtcaag aagcctgttc tccatcgccc tgagtcttga aacggcataa ccgtagtcgg
     1381 cagggtcggc catagtcgga acctccctaa cggctgggaa agaggcgatc gacaacttct
     1441 gcctccaagt cttccttaac ccgaccgagg aggacttccc aggtgcagtt gatatctatc
     1501 ctgcccttcc tgaggacgaa ccccccggac ccttggaccc tgtcctggga aagggtcagg
     1561 ttgtattgcc tttgggcgtt cacctcatcc agccactttt gatccaaaac cttttctccc
     1621 ttgcacagaa aaacctcttc atccccggaa acctccgaag acccgagcag cgctgtcatg
     1681 aaacctgcat acctgtccct gggaaggctg gataggatcg ccatggcctt ctcatagaca
     1741 aggtctacca gttctctttt cgctcccagc atgagctttc tgacttcgag atcggccacg
     1801 atcgtttttc ttttccgcac ttcgccctct tcatggttga tcctctcctg gagctttttg
     1861 tcaagcattt cactttcacg cttggccgtg acgaggatct cgtcagcctg ggcacggaac
     1921 tgtccaagga tcccctccgc ctcgttcctg gcatcggctt cgatctttgt tttaatgtct
     1981 gctaaggaca tctatctcac cccgttgtca taaaaccggt cagatctgga tgctgttcag
     2041 gagtattatg ctcatcaaca aggcgagaac ggcgtaagtt tcgaccatgg caggaaggat
     2101 gaccgccttt cctgtctcct caggtctctt ggctatcatc tggatactcg ctgcggaagt
     2161 cttgccctgg gcaatggccg aatagtaccc ggcgatggca atgggaaggc aggcgaagag
     2221 gactcccaat ccctgccaaa cattgacgga aacgacggcc ccacctagaa gtccaacctt
     2281 gaggatcgcg aaaaaggcag tgaggagccc gtagatgccc tgagtcccgg ggagggcctg
     2341 gagcaaaagg acgagaccga acttgccggg gtcttccgtc atgatgccag caccggattc
     2401 cccggcgatg ccgaccccta tcgccgaacc tgaacctgaa aacccggcgg caagtgccgc
     2461 gccaagtacc gcaagcatga tcccaagttg atccattatt gaagcacact cctctcaaag
     2521 aatgctaaaa attgaataga acagtatctt ttctaaaaac ccggaccttc gactccctgc
     2581 ccggttattc tcgtgaattt cgtggccacc ctcagcgggt tgaaagccct ccccccggct
     2641 gaatagaact tgctgaagaa ttcaacgtac tggaggcgca gggaatggat aaaagaaccg
     2701 agtacgttca caacgacact gaaaaggtgg ccgccgaaga atatcaggaa ggccaggacc
     2761 cacccaacgt aaggtatccc ggaaacgagg atcgtaagag tgttgccgat catggctatg
     2821 gccgaggtgg ccagtcccag cgcgagaaga cggctgtaac tcaggacatc ccccaggtag
     2881 gaagttacat tgtagaggct cagtaccccg gatactacct ttctgatcac tccaggcttg
     2941 tccctgccct gggttgccac gagggtcagg gctccgacga cggcaagcgc cttgcctgcg
     3001 aaaagcgttg tgccaccccc tactcccaaa gaagaagccc cccaaaggag aaaccctacc
     3061 agaagagcca gccacccacc ctggtcggcg aaagcaccca tcatgtctcc cttccggaaa
     3121 gcatcccaaa aggcgatcaa tatgccgaag aagatctgga ccacccccag gccgagcgca
     3181 atagccagaa aggtaatggg gtcatccatg ggcttgatga ggacaaggct gtcctttaag
     3241 gacttaatag gggcgaaaat accgaacacg tcgatcatgt caccgagcca actccccgtc
     3301 agggcaccca caagaacggt ggagatactg cccaaaagga ggaggacaaa gaaatttttt
     3361 gtgtcccccg aaagtctgta ccgcaagagg gcgtaaagca ggaaagcacc caggacaaga
     3421 ccgtaacctc catcccccag gcacatgccg aagaagagga agaagaaggg cgccatcagc
     3481 accgttgggt ctatgcccga gtaggacggc atcccgtaaa gctttgtgag cggagagaaa
     3541 gggatggccc atcgggggtt ttcaagtatt atcggcgggt tgtcctctgg ggatggatcc
     3601 tcggaataaa tatccaccag gcctttgcaa ggctccaggg ccttttcgac atccttcatc
     3661 ttgcccgcgg ggatccaaaa ccacaagacg gatactttct ccgaggagac cccggaatcg
     3721 cccgcctcgt ttcttgctgc caataccgcc aggtagtcac tcagattcct taccaccggc
     3781 accacttctg ttgccattcg tgaagcctca tcttcggcgg aggcgatctc ctcagcgagt
     3841 tctccccgcc gcttcgccaa cctttctatc tctttggcgg gcttatcttc gagccccggg
     3901 ggacagtcta tcctggagaa accagccctg gcggcggatt ccattacttc ctgttcaaaa
     3961 acccggggga atatcaggac tgccatggcc tcggtttcct tcgccgccgg ggaggagagg
     4021 aataactcgc cctcaccatt cacaacgctg gaaaagtgtt ctgcgaggct tgcctttcgt
     4081 cccgtgggta tagaacccat aacacccatc actttctcgg tccccctggt gatgacccaa
     4141 agaggcagtc ccaactcctt gatgggttcc aggagatgga gttgattggc tatctgtccg
     4201 gtctccgatc gcatctccga tatgcttcgt tcaagatttc gtgcttttga agatagggcg
     4261 gagagatctg tccgggctct cagtgaggcc agttctgtca atgtaacggg gggtttgggg
     4321 ccgagaagtc tttccagtgt cccgcctttt tcggcgtaaa ggggttcgag gaaccttagg
     4381 aggaatcggc agtcactaat gcaggtttcc agttccgagg acctgcctgg cttgatcctt
     4441 tcggggggcg tcgagccgag acctccctcg ggtcggctct ccccatcggg aggaatgatc
     4501 tcacaacatc cgaggccctg aagtgcggcg atggttgtat ccaagctatc gtgatgagag
     4561 acgatgcgga cttttttaac cttcgtcact gccatatcgt gacatcacct cttctgcaac
     4621 ccaatcagcc acagcgtcca ccttgccttt attgctctgg accagtcctt cagcttcttt
     4681 tctgcctttt tcaatagttt tttccgattc cctgtcggcc tcttcttcaa gtttgagggt
     4741 cgcatccctt aggcttcggt gatacctttg tcttgatccc ttggccaggt tttctgcttc
     4801 ggccctggcc tgggccaaaa cccgcgctcc ttcagcacgg gcgtcttgga tgatctgcct
     4861 ggctttgacc tccgcttcgg ttatttcttc cgcgatgttt ttcggcacac ctattacctc
     4921 ccagcatgca ttatactgta taaggaaatt ttttaacaaa cctacaagat tttaatagcc
     4981 tcccccatat catgtcaact cgacaaggcc gagttcattt tactggcggc cgggatccag
     5041 cgggatgaac cttcccggag tcttcgtttt ggagggttca aaaacattac ctacggggac
     5101 acgataaaga ataacataaa aaaa
//
LOCUS       DAOSRG010000061         5119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14841_length_5119_cov_10.123025, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000061 DAOSRG010000000
VERSION     DAOSRG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14841_length_5119_cov_10.123025"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..238
                     /locus_tag="PLM30_03385"
     tRNA            161..238
                     /locus_tag="PLM30_03385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Pro,seq:ggg)
     gene            326..1195
                     /gene="rsmA"
                     /locus_tag="PLM30_03390"
     CDS             326..1195
                     /gene="rsmA"
                     /locus_tag="PLM30_03390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOR54247.1"
                     /translation="MQGRFSRPNTHIGQNFLIDRNILAFILGRCDVGSHDVLLEVGAG
                     KGFLTEGLLGENPLLVYSSEIDRRFEPYLGSLRTRYPNLRMLWGDALKGGLPEQLEPP
                     PNKMVANIPYHITTPLLWRVLELYAPAGLNYMLLMVQKEAAERICAPERSKDRYPLGI
                     TIQAMGKPRIVRKVPPEAFSPSPRVDSALLEVSIQKNRSLPRDRIWRTLVRAAFSQRR
                     KTLLNNLAAFLPGMRREIADRMAQAGLSGTARGEELKLEQWHSLYEILGPLLQAKKEP
                     AGDRWLPPADPDE"
     gene            complement(1207..1482)
                     /locus_tag="PLM30_03395"
     CDS             complement(1207..1482)
                     /locus_tag="PLM30_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR54248.1"
                     /translation="MTKADLISAVAKSAGITKKQAHEAVGSTFQAIVKALSKGEKVQL
                     VGFGTFEVRKRAPRKGRNPQDPTKVINIPGKKVPAFRPGKALKEKVR"
     gene            complement(1595..2920)
                     /gene="der"
                     /locus_tag="PLM30_03400"
     CDS             complement(1595..2920)
                     /gene="der"
                     /locus_tag="PLM30_03400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR54249.1"
                     /translation="MAIVAIVGRPNVGKSSLFNRIVGKRKAIVDDQPGVTRDRIYGEA
                     EHGGEMFLLVDTGGLLPEGGDPMSESIKKQVLLAVHESDLLVLVVDGKTGLTTLDREV
                     ALFLRKKDKPVIVAVNKIDDLVHENREAEGYALGFPNVIGVSAAHNRNIEELLDRIRD
                     LTPGKSPGPEEIPEDEISVAIVGRPNVGKSTLLNHLSGSERSLVTSVPGTTRDSVDSQ
                     VVIGGKPFRFIDTAGLRKKSRMDSDVEFYSLVRTHESIGRADVVVLLMEGTQVCTDQD
                     KKIVGLVEDKGKGLILAVNKWDLLEKDPGLGDRISHQVREEMPFASFAPLVFISCLTG
                     RGLHKLPEKIAMVQSNRSRWLDVSKTATLVRDVLAFERMPSDPRGRPLYIRGCKQVAV
                     NPPAFAFYVNDRDIVNRSFERTTVRKIREMADFEGTPVKVFWRSMRKGN"
     gene            complement(2947..>3441)
                     /locus_tag="PLM30_03405"
     CDS             complement(2947..>3441)
                     /locus_tag="PLM30_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOR54250.1"
                     /translation="RKSLELCDHLTVPLVCWLDADSLASAPFIDSSVRAFRAVWESAW
                     RGWGHPGRKVLIQSRRPRAGWQVGLVAGWDHFWPRELEERKKLDLPPWKYLVEITNLA
                     GNKERIKTALLKKGYEALDPGGPEGMVWLKCDELDELRGTLAPFFQISGSPRGFPRIS
                     LRSE"
     assembly_gap    3442..3541
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3542..4491)
                     /locus_tag="PLM30_03410"
     CDS             complement(<3542..4491)
                     /locus_tag="PLM30_03410"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54251.1"
                     /translation="MIVPGPWWHPLTFESERPAERGTRLSVPVAGKSRIGFATGVMAD
                     SPPQGDFKIQKVKEIIDHLPPLGWELFETVLRVGRHFLCGPGEALKVIAPAHILSGEF
                     RGELSKMAAPGAAFSENYCFLPFLGDRVEEYKSLLLSRGGGALVLFPDREGASSFWKG
                     LPPEVKKDGVLWTANSGPGARKRWDRVRMGDFRLVVGSPSAVFAPLVSLQTVIIEDEG
                     NPSYYSERFPFFNARSVAGFRARLWKGNVVYGGSIPSARSYLKGLSQPAPPQSEKFLF
                     ISMRDARNMIVPGVKDPLPVSDAILNRTLEVVRDKGTTIWV"
     gene            complement(4506..>5119)
                     /locus_tag="PLM30_03415"
     CDS             complement(4506..>5119)
                     /locus_tag="PLM30_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOR54252.1"
                     /translation="AIDPVRFISNHSSGKMGASLARTSWYRGAEVTMITGPAQEPPPH
                     GSRVINVVSAEEMYETAMLEAPSQDIIVMAAAVGDFRPSSFSKDKIKRKGEKSFILEM
                     EQNRDIAAALGAARKPGQFLVGFAAETGNAMENAREKLEKKKLDQIVVNEVSGPEGAF
                     GSDRNSVRILDSGGVLFSSEGSKDEVAEAIWDAVAQGRKDHGQ"
BASE COUNT         1059 a         1526 c         1331 g         1103 t
ORIGIN      
        1 tccgtcatcg aggccttcca gaaggacgtc gatgaaggat gggagtactt gctcaagatg
       61 tgcggtgaaa cctgggaacc ccaggcgtga tgaggggcgg cccataagca tattcattct
      121 tgaccgataa acacaccgag gatacaatat tcgtcgccta cgggacgtag cgcagcctgg
      181 ttagcgcact tgactggggg tcaaggggtc ggaggttcaa atcctctcgt cccgaccaga
      241 tatcaaaagc gggagacctt ctggttctcc cgcttttcct tcttcacaac cttaagtgcg
      301 gtcccggggc cgggagacga aatgaatgca aggacgattc agcaggccta acacccatat
      361 cggacagaac tttctcatcg acaggaatat cctggcgttc atcctgggtc gctgcgatgt
      421 agggtcccac gacgtattac tggaagtcgg ggcgggcaag ggcttcctga ccgaggggct
      481 tttgggggag aaccctttgc tggtctattc atcggagatc gaccgaagat tcgagcccta
      541 ccttggaagc ctccgaaccc gctaccccaa cctcaggatg ctttggggcg acgccttgaa
      601 gggtgggctt ccagaacagc ttgaaccgcc ccccaacaaa atggtggcca acatccccta
      661 ccacatcact accccccttt tatggagggt tctcgagctg tacgcccccg ccggcttgaa
      721 ttacatgctc cttatggtgc aaaaagaagc agctgagagg atctgcgccc cggaacggtc
      781 aaaagaccgt taccccttgg ggataaccat ccaggcgatg ggaaaaccgc ggattgtcag
      841 gaaggtccca cccgaagcat tttctccttc ccccagggtg gactccgctc ttttggaggt
      901 ttcaatccag aaaaaccgct ccctaccccg cgacaggata tggcgaaccc ttgtccgcgc
      961 cgccttctcc caaagaagaa agaccctttt gaacaatctc gccgcgttct tgccggggat
     1021 gcgccgggaa atagccgaca ggatggcgca ggccggcctt tcagggaccg ccaggggtga
     1081 agaattgaaa ctggagcaat ggcactccct ttacgagata cttggtccac ttctccaagc
     1141 aaaaaaagag ccggcaggag accgatggct cccgccggca gatccggatg aataagatcg
     1201 gaagggttag cgaacttttt ctttcagcgc tttcccagga cggaaggccg gaaccttctt
     1261 ccctgggatg ttgatgacct tggtgggatc ctgagggttt ctccccttcc tgggggctct
     1321 ttttctgacc tcgaaagtac cgaagcctac caattggact ttctccccct tggaaagagc
     1381 cttaacgatc gcctggaacg tggaacccac agcctcatgc gcctgtttct tggtgatgcc
     1441 cgcagatttc gcgaccgcgc tgatcagatc cgccttagtc actttgcccg cacctccaac
     1501 tcttcaggtt gttaactagg ccgtcggacc tctagctgac ggccagatac actaatatca
     1561 tctgccgaag aagcggtcaa gtgctaaatg aagatcaatt accctttctc atcgatctcc
     1621 agaatacttt gacaggagtg ccctcgaaat ccgccatctc cctgatcttc ctgaccgtgg
     1681 tcctctcaaa ggaacgatta acaatatccc tgtcattgac atagaaggca aaggccgggg
     1741 ggttcactgc cacctgcttg cagcccctga tatacagggg cctgccccga gggtcgctgg
     1801 gcatcctttc aaaggccagc acatcccgga caagggttgc cgtctttgag acatccagcc
     1861 atcgtgatcg gttggactgg accatggcta tcttttcggg caacttgtgt agccctctgc
     1921 ccgtcaggca tgagatgaag accagggggg cgaagctggc gaagggcatt tcttccctga
     1981 cctggtggga gatcctgtcc ccaagtccag gatccttctc gagcaggtcc catttgttga
     2041 ctgcgagaat cagtcccttg cctttatctt ccacgagtcc gacgatcttc ttgtcctggt
     2101 cggtacaaac ctgggtccct tccatcagca ggacgaccac atccgccctc ccgatgcttt
     2161 catgggtcct gaccagggag taaaattcca catcggagtc catcctgctt tttttcctca
     2221 ggccggccgt atcaatgaaa cggaaaggtt taccgccgat aacgacttgg gagtccaccg
     2281 aatccctcgt tgttccgggc acagaggtca ccagggatct ttcagaaccc gaaaggtggt
     2341 tgagaagggt cgatttacct acgttaggcc gacccactat cgccaccgag atctcatcct
     2401 cgggtatttc ttcgggtccg ggactctttc caggggtcag gtccctgatc cggtccaaca
     2461 gttcttctat attccggttg tgagcggcgc tgacccctat tacgtttgga aaacccaggg
     2521 cataaccctc ggcttccctg ttttcatgca cgagatcgtc gatcttgttc acggcgacga
     2581 tgacgggttt gtcctttttc ctcaggaata gtgctacctc cctgtcgagc gtcgtcaacc
     2641 cggtcttgcc gtcgacgacg agcaccagga ggtcgctctc atggacagcc agaagaacct
     2701 gtttcttaat gctctccgac atggggtcgc ccccttcggg aagaagccct cccgtgtcga
     2761 ccagcaggaa catctccccc ccgtgttcag cctcaccata gatcctatcc cgggtcacgc
     2821 cgggctggtc gtcgacgatg gcctttctct taccgacgat gcggttgaag agggatgact
     2881 tacccacgtt gggcctcccg acgatggcca cgatagccat gaacgatctc ttcctttcct
     2941 tgtctcctat tcggaccgga gggatatcct ggggaacccc ctgggagaac ccgatatctg
     3001 gaaaaagggt gccagtgttc cccttaattc atccaattcg tcgcacttga gccagaccat
     3061 accctctggg ccacctgggt caagggcttc atagcccttc ttcagcaggg cggtcttgat
     3121 cctttcctta ttcccggcga ggttggtgat ctcaacaagg tatttccagg ggggcaggtc
     3181 cagctttttt ctttcttcaa gctctcttgg ccaaaaatga tcccaacccg ccacaaggcc
     3241 aacctgccat ccggccctcg gtcgccggct ctggatgagg accttccttc ccgggtgccc
     3301 ccaccccctc caggccgatt cccaaacagc cctgaaggct cgcaccgagg agtcgataaa
     3361 gggagccgac gcgagagaat ccgcgtcgag ccagcatacc agtggaacgg taaggtgatc
     3421 gcacagttcc aacgatttcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nacccagatt gttgtcccct tgtcgcgcac aacctccaag gtgcggttca gtatcgcgtc
     3601 gctgacaggc aaggggtcct tcacgccggg aacgatcata ttcctggcat ccctcataga
     3661 gatgaacagg aacttctctg actggggcgg ggcaggttgc gaaagaccct ttaggtagct
     3721 ccgagccgat ggaatgctcc cgccatagac gacattcccc ttccagaggc gggccctgaa
     3781 accggctacg ctccgggcat tgaagaaggg gaacctttcc gaataataag aggggttgcc
     3841 ctcatcctcg ataatgaccg tctgcaggga gaccaagggg gcgaatacgg cggaaggaga
     3901 ccccactacg aggcggaagt cccccatcct gacccgatcc cacctcttcc tggcgccagg
     3961 acccgagtta gcggtccaca agaccccgtc tttttttacc tccggcggga gtcctttcca
     4021 aaaggaggaa gctccctccc gatctgggaa aaggacaaga gccccgcccc cgcgtgaaag
     4081 aagaaggctc ttgtactctt ccaccctgtc tccgaggaag gggaggaagc aatagttctc
     4141 actgaaggcc gcaccgggcg cggccatctt cgaaagttcc ccacggaact ctcccgaaag
     4201 gatatgcgcc ggagcgatga ccttgagggc ctcgccgggg ccgcaaagga aatgccgacc
     4261 cactcggagg accgtctcaa aaagctccca gcccagcggg gggaggtgat cgatgatctc
     4321 cttcactttc tggatcttga agtcgccttg ggggggggaa tccgccatta cccccgtcgc
     4381 gaaaccgatc cttgacttcc ctgcgacggg gaccgacagc ctggtgccgc gttcagcggg
     4441 tctttccgat tcaaaggtca ggggatgcca ccaaggcccg ggaactatca cctccaggaa
     4501 cactgtcact ggccgtggtc cttgcgcccc tgggccacgg cgtcccagat cgcttcagcc
     4561 acctcatcct tagagccctc ggaggagaaa agcactcctc cgctatcgag gattctgacg
     4621 gaattcctat cggacccaaa ggctccctct gggccactga cttcgttgac cacgatctgg
     4681 tcaagctttt ttttctccaa tttttcgcgg gcgttctcca tagcgttgcc ggtctccgcc
     4741 gcaaaaccga caaggaactg ccctggtttt ctggccgccc caagagccgc cgctatgtcc
     4801 ctgttctgtt ccatttccag aataaaagac ttttcccctt ttcgcttgat cttgtccttc
     4861 gaaaatgaag aggggcgaaa gtcccctacg gccgccgcca tgacaattat gtcctgggag
     4921 ggcgcttcca acatggcggt ttcgtacatc tcttcggccg atacaacatt tatgaccctc
     4981 gaaccgtggg gcggaggttc ctgggcgggc ccggtgatca tcgtgacttc ggctccacgg
     5041 taccaggaag tcctggccag ggaggccccc atctttccgg agctgtggtt gctgatgaac
     5101 ctgacgggat cgatagcct
//
LOCUS       DAOSRG010000062         5116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14859_length_5116_cov_8.466113, whole genome shotgun sequence.
ACCESSION   DAOSRG010000062 DAOSRG010000000
VERSION     DAOSRG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_5116_cov_8.466113"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLM30_03420"
     CDS             complement(<1..520)
                     /locus_tag="PLM30_03420"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOR54253.1"
                     /translation="MGARDLLLEIGTEEIPSRFIPPTLSELATIAEEELRSERLVFEN
                     TQTLGTPRRIVLITRSLEEKQQDKSDEFKGPAWASAFDPSGNPTKAARGFAKSKGVDV
                     DSLERRDFGGQEFAVAVKREEGRPSSAILPAVLEKVIKRLSFPKSMYWDRTFQRFARP
                     VRWLLALYGDELL"
     gene            complement(510..1415)
                     /gene="glyQ"
                     /locus_tag="PLM30_03425"
     CDS             complement(510..1415)
                     /gene="glyQ"
                     /locus_tag="PLM30_03425"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOR54254.1"
                     /translation="MNLQEIMMRLQRFWAEQGCVIQQPYDIEVGAGTMNPATTLRVIG
                     PEPWRVAYVEPSRRPADGRYGQNPNRLQHYYQFQVIIKPAPEEIQETYLESLRTLGIE
                     PADHDIRFVEDDWESPTIGAWGLGWEVWLDGMEITQFTYFQQVGGIDMDPVAAEITYG
                     LERIAMYVQKVDNVFDLEWVGKISYGDIHHQGEVEHSIYNFEVSDTDMLFRLFSLYEA
                     EAKSALSMGLVLPSYDYVLKCSHVFNLLDARNAISVTERTGFISRVRALAGSCCQSYA
                     RQRQEMGFPLMGKFDDRIERKNDGC"
     gene            complement(1443..2258)
                     /locus_tag="PLM30_03430"
     CDS             complement(1443..2258)
                     /locus_tag="PLM30_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOR54255.1"
                     /translation="MFTWTFGSRSSRDGETPKGNSGEWAIPRRDAFLEGKPGQGLLRK
                     QGVVLRRKDSNEGDRSIYVLLKDEGPVWLIAPGAARGKVRFGGATDPLVWGTFHIYRG
                     PNRWYLRDVDVRKDFLPLRKHPSRIRRAAEWAASLVKYTLPGHASDDLLPIFYWSLCV
                     LESGRLREDLAGWRFYWRWLRNWGLAPDLERCQECGVPLGEALFCGGSFFCAQCSGGR
                     QGLRLSGQDRGELFAVSVLGHERVLAMEGLPGIDPGKAESCTRMLIRTLSDRM"
     gene            complement(2176..3087)
                     /gene="era"
                     /locus_tag="PLM30_03435"
     CDS             complement(2176..3087)
                     /gene="era"
                     /locus_tag="PLM30_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOR54256.1"
                     /translation="MSEGPAYRSGFVAVVGRPNVGKSTLVNALVGGKAAIVSDKPQTT
                     RNSIRAVLNDPRGQIVFVDTPGLHRPLHRLGRFLEKQAAASLEAADVVCFMVEAGDNR
                     VGKADGIILDLLEKVSVPVVLVVNKVDAHDHDHGPDGWECFTRMRGFAHVLTVSALEG
                     RNLGLLVETLFDLLPPGPPIFDDDRLVDCTERFLAAEVIREKVLEQTEQEVPHSVAVV
                     VEEFRSPDEYPERRNLLVRATLFVERPGQKAILIGKGGAKLKGIGELARKELEEVFGY
                     DVYLDLWVKVKPRWRNSERELRRMGYT"
     gene            complement(3084..3515)
                     /gene="cdd"
                     /locus_tag="PLM30_03440"
     CDS             complement(3084..3515)
                     /gene="cdd"
                     /locus_tag="PLM30_03440"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOR54257.1"
                     /translation="MKGPSGRDPLKREELEDLMEQARDARDRSYAPYSGFAVGAAVLS
                     GSGEVFRAGNLENTSYGLTVCAERNAIALMVASGRRDPVAMAIAGPEGVPCPPCGACR
                     QVLAEFNPAMAIILEDEAGGFEVFSLEYLFPRPFRLGEGGE"
     gene            complement(3512..4795)
                     /locus_tag="PLM30_03445"
     CDS             complement(3512..4795)
                     /locus_tag="PLM30_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR54258.1"
                     /translation="MQEEIIRSILILAVLLLFSAFFSSSETAITSIGRGKLLAIQEKN
                     PKRRKGIDWLISDMPRALTVILIGNNLVNIAASAVATQVALSLIGAKGLLLAVAFTTV
                     VIVIFGEILPKSVAILKPDGIVSGALPLLRFINMIFSPLVWVTVGIVKFFGSLLKVDL
                     SSQHPFVTREEIEQMVNIGEASGVFEEEERRMIHGVISFEETRVYEIMIPRTDMVAVP
                     GDTDISESLPVFRECGHSRLPVYGKSPDHILGILYVKDLIGAFTSGDPSIKVSSLARE
                     ALFVPETMRLADLFDVMRGRRVHMAIVIDEYGGTAGLVTLEDLIEEIVGEIQDEYDDE
                     SALIQKEPDGSYLVRGQMGLEDLSEAVGYPFESSDMDSVGGLVLSLAGRFPDKGQRLT
                     YGHWEFQVLEVEDHRIKLVKMTPLDDLYRIGEVSE"
     gene            complement(4817..>5116)
                     /gene="ybeY"
                     /locus_tag="PLM30_03450"
     CDS             complement(4817..>5116)
                     /gene="ybeY"
                     /locus_tag="PLM30_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR54259.1"
                     /translation="LSFPIHASGGKFTCPAPLPECLLGDIMVCLPRVLENALEAGHSP
                     VKELSLVLVHGLLHLLGWNHETEEGRREMWDIQGHYLERVERCLTPPERRADPGA"
BASE COUNT          989 a         1648 c         1440 g         1039 t
ORIGIN      
        1 tcagcagttc atccccataa agggccagaa gccatcggac gggcctggcg aaacgctgaa
       61 aggtccgatc ccagtacatg ctcttgggga aggacagcct ttttatcact ttttcaagga
      121 cggctgggag gatggccgac gacggccgtc cctcctccct tttgacggcc acggcgaact
      181 cctggccgcc gaagtccctt ctttcaaggg aatccacatc gacgcccttg cttttggcga
      241 aaccccgggc ggccttggtg gggttgccgc tggggtcgaa ggcggatgcc caggcgggcc
      301 ccttgaactc atcgctctta tcctgttgtt tctcctccag ggaccgggta atcagaacga
      361 tcctccgggg tgtccccaat gtctgtgtgt tctcgaagac gagcctttcc gaacgcaact
      421 cctcctcggc gatggtcgcc aactcggaaa gggtcggcgg gatgaacctg gaggggatct
      481 cctccgtccc gatctcaaga agcaggtctc tagcacccat cgttcttcct ctctatcctg
      541 tcgtcgaatt tacccatcaa gggaaaaccc atctcctgtc tctgcctggc gtaggactgg
      601 cagcaggagc ccgcaagggc acggaccctg gaaatgaacc ccgtccgctc cgtgacgctt
      661 atggcgttcc tagcgtcgag gagattgaaa acatgggagc acttcagaac gtaatcatag
      721 gagggcaaca ccaatcccat ggaaagggcg cttttcgcct cggcctcgta cagggagaaa
      781 agcctgaaaa gcatgtccgt atcggaaacc tcgaaattat agatggagtg ctccacttct
      841 ccctggtgat gtatatcccc gtaagagatc ttccccaccc attcgaggtc gaagacgtta
      901 tctaccttct gtacgtacat ggctatccgt tctaacccgt aggtgatctc cgccgccacg
      961 ggatccatgt cgatgccgcc aacctgctgg aagtaggtga actgggtgat ctccatgccg
     1021 tcgagccata cctcccaacc caggccccag gccccgatgg tgggtgattc ccagtcgtct
     1081 tcaacgaaac ggatatcatg gtcggccggt tcgatcccca aggtcctgag gctctcgagg
     1141 taggtctcct ggatctcctc cggcgcgggt ttgattatta cctggaactg gtagtagtgc
     1201 tggagcctgt tcgggttctg cccgtacctc ccatcggcgg ggcgccttga tggttccacg
     1261 taggcgaccc tccagggttc ggggccgatc actcgcagcg tcgtggccgg gttcatggtg
     1321 ccggccccga cctcgatatc gtagggctgc tggatcacgc atccctgttc agcccaaaac
     1381 ctttgaaggc gcatcatgat ctcctgaagg ttcaccccgg tattcctccc tctggttcga
     1441 cgttacatcc tgtccgacag ggtccttatc agcatcctcg tgcaggattc ggccttcccg
     1501 ggatcgatcc cgggaaggcc ctccatcgcc aatacccttt catggcccag caccgaaacc
     1561 gcgaagagct caccccgatc ctggccgctg agcctcaacc cctgcctgcc gccggaacac
     1621 tgggcgcaaa agaacgatcc cccgcagaaa agggcctctc cgagcgggac accgcattcc
     1681 tggcagcgct cgaggtcggg ggcaaggccc caattcctta accatcgcca gtagaatcgc
     1741 cagcccgcca ggtcttccct gaggcgaccc gattccagga cgcaaaggga ccagtagaaa
     1801 atgggcaaaa ggtcatcgga agcatgaccc ggaagcgtat attttaccaa agacgctgcc
     1861 cactcggcgg ccctccttat gcgggagggg tgcttcctca ggggcagaaa atccttccgg
     1921 acgtccacat ctctcaggta ccaccggttg ggcccccggt aaatgtggaa agtaccccaa
     1981 acaaggggat cggtagctcc cccgaacctc accttgcccc tggcggcgcc gggagcgatc
     2041 agccagaccg ggccctcgtc ctttagaagg acgtatatgc tccggtcgcc ctcgttggaa
     2101 tccttccttc gaaggacgac cccctgcttt cggagcagac cctgaccggg cttgccctcc
     2161 aggaaggcgt cccttctagg tatagcccat tctccggagt tccctttcgg agtttctcca
     2221 tctcggcttg accttgaccc aaaggtccag gtaaacatcg tacccgaaaa cctcctccag
     2281 ttcctttcgc gcgagttctc cgataccctt tagtttcgcg ccgcccttcc cgatgaggat
     2341 ggccttctgg cccggcctct cgacgaaaag ggtcgctcgc accagcaggt tcctcctttc
     2401 gggatactcg tcgggggacc ggaactcctc aacaacaacg gcgacgctgt ggggcacctc
     2461 ctgctcggtt tgctcgagta ctttctccct gatgacctcg gccgccagaa atcgttcggt
     2521 gcagtccacc agcctgtcgt cgtcaaaaat gggggggcct ggaggtaaga ggtcaaaaag
     2581 ggtctccacc aggaggccca ggtttcgccc ctcaagggcc gacaccgtca ggacatgggc
     2641 gaaccccctc atccgggtga agcattccca gccgtcggga ccatgatcgt ggtcatgggc
     2701 gtcaaccttg ttcacgacga ggacgacggg caccgagacc ttttccaaca ggtccaggat
     2761 gatcccgtcg gccttgccca ccctgttgtc gccggcctcc accatgaaac atacgacatc
     2821 ggccgcctca agggaagccg ccgcctgctt ttccaggaaa cgacccagcc tgtggagggg
     2881 cctatggagc ccaggggtat cgacaaagac gatctgcccc cgcgggtcgt tgagcaccgc
     2941 cctgatgctg ttcctggtgg tttggggctt gtccgagaca atggctgcct tcccgcccac
     3001 caaggcgttg accagcgtcg atttccccac gttgggcctg cccacaaccg ccacgaaacc
     3061 ggaccggtac gcagggcctt cgctcattcg ccgccctctc ccagcctgaa gggcctgggg
     3121 aaaaggtatt ccagggagaa aacttcaaac ccgccggcct cgtcttcgag gatgatggcc
     3181 atggcggggt tgaactccgc caggacttgc cggcaagctc cgcagggagg gcaggggacg
     3241 ccctcgggcc ccgcgatggc catggccaca gggtcccttc tccccgaagc caccatcagg
     3301 gcgatggcat ttctctcggc acagactgta agaccgtaac tggtgttctc caggttcccg
     3361 gcccggaaga cttccccgga accggagagc acggcggccc ccacggcgaa ccccgagtag
     3421 ggggcgtagg acctgtcccg cgcgtccctc gcctgctcca tgaggtcctc gagttcctcc
     3481 cttttgagag ggtctctccc tgagggcccc ttcattccga tacctccccg atcctgtaga
     3541 ggtcatccag gggggtcatc ttgaccagct tgatccggtg gtcctccacc tccaggacct
     3601 ggaactccca atggccgtaa gtgagccttt gccccttgtc ggggaagcgc ccggccaggg
     3661 agaggaccag ccccccgacg ctgtccatgt cactggattc gaaggggtat cccacggcct
     3721 cactgaggtc ttccagcccc atctggcccc tgacgaggta gctgccgtcg ggttccttct
     3781 gtatcagggc gctttcatcg tcgtattcat cctgtatctc cccgacgatc tcctcgatga
     3841 ggtcctccag ggtgaccagg ccggcggtcc cgccgtattc atcgatgacg atggccatgt
     3901 ggacccttcg gcccctcatg acatcgaaaa ggtcggccag cctcatcgtc tcgggtacga
     3961 aaagggcttc cctcgcgagg gaggagacct tgatcgacgg gtcgcccgac gtgaaagccc
     4021 ctatcaggtc cttcacgtaa agtatgccga ggatatggtc ggggctcttc ccgtaaaccg
     4081 gtagccttga gtgcccgcac tccctgaaga ccgggaggga ttcgcttata tcggtgtcac
     4141 cgggcaccgc caccatgtcc gtcctgggta tcatgatctc gtagaccctg gtctcctcga
     4201 aggagatcac gccgtggatc atcctgcgtt cctcctcttc gaagacgccg gaagcctccc
     4261 ctatgttgac catctgctcg atctcctccc tggtgacgaa ggggtgctgg gaggagaggt
     4321 ccaccttcag gagggacccg aagaacttga cgatccccac ggtgacccag acgaggggcg
     4381 aaaagatcat gtttatgaac cggaggaggg gcagggcacc ggagacgatc ccgtcgggtt
     4441 tcagtatggc gacgctcttg gggagtatct caccgaagat cactattacc accgtcgtga
     4501 aggccacggc cagaaggagc cccttcgccc ctatcagcga taacgccacc tgggtggcca
     4561 cggcgctggc agcaatgttg acgaggttgt tacctatcaa gatgaccgtc agggcccggg
     4621 gcatgtccga gatgagccag tcaataccct ttcttctctt ggggttcttc tcctggatcg
     4681 ccagcagttt gccgcggccg atggaggtga tcgccgtctc gctgctgctg aaaaaagccg
     4741 agaagaggag aagaacggcc aggatcagga tacttcggat gatctcttcc tgcacctgca
     4801 actcaatcct ttcttgttat gccccagggt ccgcccttcg ctccggggga gtgaggcaac
     4861 gctccactct ttccaggtag tgtccctgga tatcccacat ttcgcgcctt ccttcttccg
     4921 tctcgtggtt ccaacccaaa aggtgcaaaa gtccatggac caggacgagg gatagttcct
     4981 tcaccggaga gtgacccgcc tccagggcgt tctccagcac ccgtgggagg cagaccatta
     5041 tatcccccag caagcattcg ggaagggggg ccgggcaggt aaacttccct ccggaggcat
     5101 ggatcggaaa ggacag
//
LOCUS       DAOSRG010000063         5087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_14948_length_5087_cov_10.148847, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000063 DAOSRG010000000
VERSION     DAOSRG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14948_length_5087_cov_10.148847"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1328
                     /locus_tag="PLM30_03455"
     CDS             105..1328
                     /locus_tag="PLM30_03455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR54260.1"
                     /translation="MDYESLLAKVPGHLKNPAEFRTAGVKGKGKLAVGFGLVKTLGEE
                     AAKLGSGKALLVTDKNMVNLGIHRFAVDPLEKAGFSVQVFDEVEPEPHVETCQKVQDL
                     TRKEGFSVVIGLGGGSPMDVAKAASLAPGNPREMIDYMTGTPLEGEGLPCILVPTTSG
                     TGSEVSPYIVTSKGDKKLFIGNPLVYGTVALVDPLLTVTMPPKVTACTGLDALSHGVE
                     GMIGKTNPLTETFTLKCVEYVFKYLERAVADGNDLEARYHMSFASLFGMMSYTQGGGL
                     YAHSMSYILTIDSGVPHGLGCGLALPYTFMFNLDNVAPILVKMAPLAGVDASGNPKEV
                     GRKVAARFKKLVSDVGIPSSLKELGVPEKEVERFASDMVAKYYRVNNPRPMNPEEGLA
                     LVRSMWEGNLTEIKG"
     gene            1325..>2301
                     /locus_tag="PLM30_03460"
     CDS             1325..>2301
                     /locus_tag="PLM30_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOR54261.1"
                     /translation="MIPLYGYHGVIALVDLAARKVQRIEIEEDIARKYIGGSGIGTHL
                     LVKYGLPAMDPLDPRSPLIYMTGPFMGTGIPTSGRHQILARSPLTGGFGEADCGGSFG
                     FHLKRSGFDGLVITGRSPSPVVVVAKDDEISFYDGGHLWGKDTFETEETLSREMPWGI
                     GVSCIGPAGEKQVPLAGILHDGVHARAAGRGGLGAVMGSKNLKAIVAKGKRLPAIADR
                     KALNELIIESNNRMKEKGAGMRLYGTAGGMENAEKFGDLPIRNWKQGSWPEGAKAISG
                     QEMAKGLLTGDYGCIACPIRCGREVTLDGLKMGGPEYESLAMLGSACLV"
     assembly_gap    2302..2401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2402..2767
                     /locus_tag="PLM30_03465"
     CDS             <2402..2767
                     /locus_tag="PLM30_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR54262.1"
                     /translation="LMAVADSLKVCKFSISVGTRVEEYARWLSLVTGWDVSVEELLLT
                     GERVFTLKRLYNAALGIGREEDTLPERILKEPRGTGESADTLPDLEKQLDEYYRFRGW
                     SGEGLPTDETLERLGLERL"
     gene            2919..3947
                     /gene="dctP"
                     /locus_tag="PLM30_03470"
     CDS             2919..3947
                     /gene="dctP"
                     /locus_tag="PLM30_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOR54263.1"
                     /translation="MSKRVKLSVSLVVAVCMAVFLAGAWSSPSEAKAEITLRMAGQSP
                     ADHQSTLGMQKVSKWVAEATNGRIDIKTYPSSQLGDYTLVYEELIRGTIDMALISIPS
                     QFDSRLELIYVPYLMRDYGEAAEVFKQGGWLFNKMAELHGELGVLFLGFNVEGFGGLG
                     LTKEPKEVLNPLVKKDLLLRVPPMDTFKIGADDMGYTTVSVPYAELYTALQTGIAEGW
                     TGGAPPHSYQGFRDVLKYYYQINNFVETESYLMSKVTWNKLSEADRKIIADACARAAR
                     ESIEISEREDREYFDKLQDYGVKSFWYTAEDVKDLAAHARKVTWPKLEKRLTKELIDE
                     MMEAYNVK"
     gene            4076..4639
                     /locus_tag="PLM30_03475"
     CDS             4076..4639
                     /locus_tag="PLM30_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOR54264.1"
                     /translation="MTGTCGYEEPHPIKIHNFLWEFLLKFQRFVLIVSSLFVVASIVL
                     TVILRYIFKTDLYGLEEIVVIFAFWLYFMGGSYGSYTGTHITADLVSVFFPEGKLKSS
                     VIAFSSTITFLFSLLFSWWGLDFFMFGVKMSARTPVWRIPMVVSYTAVFLGMVLMTFY
                     FFLAFLEDIKKLRSHFGQGTPQAEGNR"
     gene            4654..>5087
                     /locus_tag="PLM30_03480"
     CDS             4654..>5087
                     /locus_tag="PLM30_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR54265.1"
                     /translation="MVLFSLVLLVITLIIGVPVPFAFLVATTLLVITGGYSPSFLLPY
                     GFSKMSTIVLLAIPLFIMAGGIMERGNIGDKLVDLVDLFVGRIKGGLGVVAVVSCAVF
                     GSITGSACATLSCIGSIMFPRLEKAGYPRGHSAALLANASVLG"
BASE COUNT         1041 a         1442 c         1526 g          978 t
ORIGIN      
        1 aaaaaaattt cgtatcattc atacattgtt cgatgaacct cggttgacta atatgtaaga
       61 cggcgttttt tcgaagaatc tcgaaaacga aaaggggtgc ggctatggat tacgaatcct
      121 tattggcgaa agtaccaggg caccttaaga accctgccga gttcagaaca gccggtgtaa
      181 agggcaaggg gaaactcgcg gtaggtttcg gcctcgtgaa gaccctgggg gaagaggcgg
      241 cgaagctggg gtctggcaag gcgctgctcg tgaccgacaa gaatatggtc aacctcggta
      301 tccaccggtt cgccgtcgac ccgctggaga aggccggttt ctccgtgcag gtctttgatg
      361 aagtcgagcc cgagccccac gtggagacct gccagaaggt ccaggacctc acgagaaaag
      421 agggcttctc cgtcgttatc ggtcttgggg gggggagtcc catggatgtc gccaaggccg
      481 cttccctcgc ccccggcaac cccagggaga tgatcgacta catgaccggc accccccttg
      541 aaggggaagg cctgccctgc atcctggtcc ccaccacttc cggtacaggc agcgaagtca
      601 gcccctatat agtgaccagc aagggcgata agaagctctt catcgggaat cccctggtct
      661 acgggaccgt cgcactggtg gacccgctgc tcacggtcac catgccgccc aaggtcacgg
      721 cctgcaccgg gctcgatgcc ctctctcacg gtgtggaggg gatgatcgga aagacgaacc
      781 cgctgacgga gaccttcacc ttgaagtgcg tggaatacgt cttcaagtac ctggagaggg
      841 ccgtcgccga cggcaacgac cttgaggcgc gctaccacat gtccttcgcc tccctcttcg
      901 ggatgatgtc ctacacccag ggcggcggcc tttacgccca cagcatgtcc tacatcctca
      961 ccatcgattc cggcgtcccc catggcctgg ggtgcggctt ggcgctcccc tataccttta
     1021 tgttcaacct cgataacgtc gccccgatcc tcgtgaagat ggcccccctg gccggtgtgg
     1081 acgcctcggg gaacccgaaa gaggtggggc gcaaggtagc ggcccgcttc aaaaagctcg
     1141 tatccgatgt ggggataccc tcgagcctga aggaactcgg cgtcccggag aaagaggtgg
     1201 agcgcttcgc ctccgacatg gtagcgaagt actaccgggt caacaatccc aggccgatga
     1261 accccgagga ggggctcgcc cttgtaaggt ccatgtggga agggaacctc acggagataa
     1321 aggggtgatc ccattgtacg gataccacgg cgtgatagcc ctggtggacc tggccgcgag
     1381 gaaggtccag aggatcgaga tcgaggaaga tatcgccagg aaatacatcg ggggatcggg
     1441 gatcgggacc cacctgctgg tgaagtatgg cctcccggcc atggacccgc ttgacccgag
     1501 gagccccctc atctacatga ccggaccctt catgggaacc ggcataccca cctcggggag
     1561 gcaccagatc ctcgccaggt caccgctcac gggcggcttc ggtgaggccg actgcggagg
     1621 gagtttcggt ttccacctga agagatcggg tttcgacggc ctcgtgataa ccggcaggtc
     1681 cccgagcccc gtggtagtgg tcgccaagga tgacgagatc tccttttacg acggcggcca
     1741 cctctggggc aaggatacct tcgagaccga ggagaccctc tcaagagaga tgccctgggg
     1801 catcggtgtg tcctgcatcg gccccgcggg tgagaagcag gtgcccctcg cggggatcct
     1861 ccatgacggg gtccacgccc gcgccgcggg gaggggaggg ctcggggccg ttatggggtc
     1921 gaagaacctc aaggccatcg tagccaaggg gaaacgcctc ccggctatcg ccgatcgcaa
     1981 ggctctcaac gaactgataa ttgaatccaa caacaggatg aaggaaaagg gcgctggcat
     2041 gaggctttac gggaccgccg gcggtatgga gaacgcggag aagttcggcg acctgcccat
     2101 caggaactgg aagcaggggt cctggcccga gggggccaag gccatatcag gccaggagat
     2161 ggccaagggg cttctcacgg gggattacgg ctgcatcgct tgtcccatcc gttgtggcag
     2221 ggaagtaacc ctcgacggtc tgaagatggg cggccccgag tacgagtccc tggcgatgct
     2281 gggtagcgct tgcctcgttg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nctgatggcc gtggctgatt cgctgaaggt gtgcaagttc tccataagcg tcgggaccag
     2461 ggtcgaggaa tacgcccggt ggctttccct ggtgaccggc tgggacgtct cggtcgagga
     2521 attgcttctg accggcgaga gggtcttcac actaaagagg ctctacaacg ccgcccttgg
     2581 cattggcagg gaagaggaca ccctgccgga gaggatcctg aaggagccca gggggaccgg
     2641 cgagtcggcc gatacgctgc ccgacctgga gaaacagctg gatgagtatt accgtttccg
     2701 gggctggtcc ggagaggggc tacccaccga cgagacactg gagagacttg gtcttgagag
     2761 actttaaaaa ttgatcgccc ctgactccct cctgtcgtgg aggatgacca aaacgtgaga
     2821 ggaggaagcg cggatcgtcg gactttacca gcggcaacgg gcatacggaa cgcaataatg
     2881 aaaccggata ccccaatact gagaggagga aaggatccat gtccaagaga gtgaagctgt
     2941 cggtgtccct tgttgttgca gtttgcatgg ctgtcttcct tgcgggagcc tggtcgtccc
     3001 catcggaagc gaaagcggag atcacgctca ggatggcggg gcagagcccg gccgaccacc
     3061 agtccacgct gggcatgcag aaggtgtcca agtgggtcgc ggaagccacc aacggccgca
     3121 tcgatatcaa gacctacccc tcgagccagc tcggcgacta taccctggtc tacgaggaac
     3181 tgatccgggg gaccatcgac atggccctca tttcgatacc gagccagttc gactcgaggc
     3241 tggagctcat ctatgtcccg tacctcatgc gggactatgg cgaagccgcc gaggtcttca
     3301 agcagggcgg gtggctcttc aacaagatgg ctgaactcca cggggaactg ggtgtcctgt
     3361 tcctggggtt caacgtcgag ggcttcggcg gccttgggct gacgaaggag cccaaggaag
     3421 tgctcaatcc cctggtcaag aaggaccttc tcctgagggt tcccccgatg gacaccttca
     3481 agatcggcgc cgatgatatg ggctatacca ccgtatccgt cccctacgct gaactctaca
     3541 cggccctcca gactggcata gccgaggggt ggaccggcgg ggcgcccccg cactcctacc
     3601 agggcttccg cgacgtgctc aagtactact accagatcaa caacttcgtt gagaccgaat
     3661 cctacctgat gagcaaggtc acctggaaca agctcagtga ggccgacagg aagatcatcg
     3721 ccgatgcctg cgccagggcc gctagagaga gtatcgagat ctccgagaga gaggacaggg
     3781 agtacttcga caagctgcag gactacggcg tgaagagttt ctggtatacc gccgaggacg
     3841 ttaaggacct cgccgctcat gcccggaagg tcacctggcc gaaactcgag aagcgcctca
     3901 cgaaggaact catcgatgaa atgatggagg cctacaacgt aaaataactg cgctaccctt
     3961 cgcggtagaa gatcagatac cgaccggtgc ggtcttcccc aaaggcatct ccgggaagac
     4021 cgcaccggaa ccttgaagac ctgcttcttt cggggaagcg gataggaggt ataacgtgac
     4081 gggaacatgc ggctacgagg aaccgcaccc catcaagatc cataactttc tctgggagtt
     4141 cctcctgaag ttccaaaggt tcgtcctgat cgtgagcagc cttttcgtcg tggcctccat
     4201 agtgctcacc gtcatcctca ggtatatttt caagaccgac ctttacggcc ttgaagagat
     4261 cgtagtgatc ttcgccttct ggctttactt catggggggg tcctacggta gttacaccgg
     4321 gacccacatt acggcggacc ttgtttcggt gttttttccc gagggcaaat tgaagtcttc
     4381 agtcatcgcg ttcagttcga ccatcacatt cctgttcagc ctgcttttct cctggtgggg
     4441 gctcgacttc ttcatgttcg gcgtgaaaat gagcgcccgg accccggtct ggaggatccc
     4501 catggtggtc tcctatactg cggtttttct cgggatggtt ttgatgacct tctacttttt
     4561 ccttgccttt ctggaagaca ttaaaaagtt gcggagccac ttcggacagg gaacccctca
     4621 ggccgaagga aaccgctgag aggagttcat cccatggtcc tattttcgct agtcctgctc
     4681 gtcataacct tgatcatcgg ggtccccgtc cccttcgcct ttctcgtcgc caccacgctc
     4741 ctggtcatta ccggcgggta ctcgcccagc ttccttttgc cctacggctt cagcaagatg
     4801 agcaccatcg tcctcctggc cataccgctt ttcatcatgg cgggggggat catggagagg
     4861 ggcaatatcg gtgacaagct ggtggacctg gtggacctct tcgtggggag gatcaaggga
     4921 ggtctcggtg tcgtcgcggt ggtatcctgc gccgtcttcg gttccataac gggcagtgcc
     4981 tgcgcgaccc tttcgtgcat aggctccatc atgttcccga ggctggagaa ggccggctat
     5041 cccaggggcc actcggcggc cctcctggcc aacgcctcgg tcctggg
//
LOCUS       DAOSRG010000064         4918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_15590_length_4918_cov_8.607650, whole genome shotgun sequence.
ACCESSION   DAOSRG010000064 DAOSRG010000000
VERSION     DAOSRG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15590_length_4918_cov_8.607650"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..696
                     /locus_tag="PLM30_03485"
     CDS             202..696
                     /locus_tag="PLM30_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOR54266.1"
                     /translation="MVIFVLSVAAYLLLAWSGGGIDPIEAGIAVALGGVMAGLARAWN
                     PGRSWTLAGLNPLRWVKFILFLAFPFLWGMTKANLDVAYRVITGKIRPGIVRIDSGLK
                     GSLAQAMLANSITLTPGTLTVDVDDSGETGVYYIHWINVTDEDPSEESVYGSFGRWAR
                     RVAG"
     gene            693..953
                     /locus_tag="PLM30_03490"
     CDS             693..953
                     /locus_tag="PLM30_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOR54267.1"
                     /translation="MIISYFAWAAAVTGFCALAMVGRLIAGPTVPDRVVALDSINTMV
                     IALMIILSLVYDSVIMVDVAIVYAALSFVGTMFIARHVEGGV"
     gene            956..1327
                     /gene="mnhG"
                     /locus_tag="PLM30_03495"
     CDS             956..1327
                     /gene="mnhG"
                     /locus_tag="PLM30_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOR54268.1"
                     /translation="MEKAALILLTIGVLFNFLGTVALYRFPDVYTRLHGTTKCTTFGS
                     IFTSAAVVVYALQMYLRTGEGRFTTLSVHVVLAILALLISNPTGAHAIARAAHRSGVL
                     PKYAVVDRLAEDHRKKGGVSA"
     gene            1324..1587
                     /locus_tag="PLM30_03500"
     CDS             1324..1587
                     /locus_tag="PLM30_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOR54269.1"
                     /translation="MNGYAHMAVLAIIVIAAFYAVWFRDLLSSVISLAVFSLLLALEY
                     FMLQAPDVAIAEAAIGAGLSTAIYIIALRACKALKSPRGGEGR"
     gene            1584..1859
                     /locus_tag="PLM30_03505"
     CDS             1584..1859
                     /locus_tag="PLM30_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54270.1"
                     /translation="MKRQVLFVVAVLVVAGVFLGALARIHPFGDTTRAPMDDYYLENA
                     QRERSVNNVVTSIVFDYRGFDTLGEAAVLFTAVCSVLALFREGSEKR"
     gene            1856..2353
                     /locus_tag="PLM30_03510"
     CDS             1856..2353
                     /locus_tag="PLM30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOR54271.1"
                     /translation="MNPLSVVVRTICDIFAWFLGIFGAYVIIHGHISPGGGFQGGAVV
                     ATMAALLLVAYGGKKVLSFVRPGIYTGMEATGLLVFITAGFLGIGTTFFYNFLAGQGG
                     LFGSVVPLGPNSGILNSAGTISLMNMAVGLEVVSGLSLILIYMFKGIRLFEGREGRSE
                     RGHDR"
     gene            2343..2690
                     /locus_tag="PLM30_03515"
     CDS             2343..2690
                     /locus_tag="PLM30_03515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOR54272.1"
                     /translation="MTGNAPFLVVGVIFLLGLYAILTERNLIKIAIGISLLEASANLL
                     LIVLGYRTGGSVPIFTLAGDVGTMVLPTPQALTLTAIVIGLATSALLLSLVMLLYRHY
                     GTLDVREIRRLRG"
     gene            2687..4252
                     /locus_tag="PLM30_03520"
     CDS             2687..4252
                     /locus_tag="PLM30_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOR54273.1"
                     /translation="MNWQMHLPALALAVPLLGAFLSLVVGLGGRYLRNAWMVCVSIAT
                     TAICIMLLQRVLAGGTMVYVMGAESWNFTLPSGLSWPIRIIIQVDAFSAFMAATSSIA
                     ALAGAFFSLRFMDKFTGLEKFSALYFLLVTGMLGMQITGDLFNFFVFLEIASVASFGL
                     IAFWRDRPEAVEASFKYMVVSTVSAMFVLIAVGFLYGRYNAVNIAAVARMLHMGYAEK
                     IALVFLVVSLAMKSGAVPMHMWTPDAYAEAPAGVTCLLVAVSQASLYGLFRVCFTLYG
                     VTAGSVVIPWTIIIMGVLSMFIGVTMAVIQKEMKRLMAYHSVSQIGYILLALGVGLLS
                     LKDPQAMADYGFTAMKGGIFHILNYTMYKGLLFLSAGAVFYSAGTRDLNELGGLARNM
                     PQTTFMFVIAAAAIAGLPPFNGFVSKLLIYQTTFAVYPLLAVAALVTSVLTLASFVKV
                     FQTAFLGPARKTFEKVVEVPASMLLGMAVLTLVIVVLTLFPGWTLSHLVEPAAKALID
                     QSGYISAVMGGGL"
     gene            4252..4590
                     /locus_tag="PLM30_03525"
     CDS             4252..4590
                     /locus_tag="PLM30_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOR54274.1"
                     /translation="MWEKVYSGFGYWDVYLWLLFFAIASAVILFIRSKGRSDYKEGTE
                     QDEIFYGSNIVPEDGADMAVPAASAYWGFVEALKPYYNWLIELHTGIASEYVGYFMLT
                     LAVVAVLVLL"
     gene            4602..>4918
                     /locus_tag="PLM30_03530"
     CDS             4602..>4918
                     /locus_tag="PLM30_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOR54275.1"
                     /translation="MNLERYLDILTKSIWVFHCNSGSCNGCDIEIVASITPRYDIERF
                     GMKLVGSPRHADALLVTGPVTKYMTGRLRRIYAQMPDPKVVIAVGNCAGSGDVFYKSY
                     NLDG"
BASE COUNT          802 a         1576 c         1456 g         1084 t
ORIGIN      
        1 cggccgggta cttcaagggt caagactagc acgtcaaggc gggttccgaa aggttttaaa
       61 aattgggggt tctcccatgg ctgttattat gtataatgca taactgtatt tactccgagc
      121 ccggccgtct tgccgggggc gcgttcatgt cctgtatcgc ttcggcgatc ttgaaagatc
      181 tggaataggg gggtgaggcg tgtggtaatt tttgtccttt ccgttgccgc ctacctgttg
      241 ctggcttgga gtggtggagg catcgacccc atcgaagcgg ggatagcggt ggccctgggg
      301 ggtgtcatgg ccggccttgc gagggcctgg aacccgggca ggtcctggac cttggccggg
      361 ttgaacccac tgcgctgggt caagttcatc ctgttcctcg catttccctt cctctggggg
      421 atgaccaagg ccaacctgga cgtggcctac agggtgataa cgggcaagat ccgccccggc
      481 atcgtcagga tcgattccgg gttgaagggg agcctggccc aggccatgct ggcgaactcc
      541 atcacgctga cgccgggcac gcttaccgtc gacgtcgatg attccggcga gacgggggtc
      601 tactacatcc actggatcaa cgtgaccgat gaggacccgt ccgaggagag cgtctatggc
      661 tccttcggga gatgggcgag gagggttgcc ggatgatcat ttcctatttc gcctgggcgg
      721 cggcggtgac gggtttttgc gcgctcgcca tggtgggcag gctcatcgcg gggcctaccg
      781 ttcccgacag ggtggtggcc cttgactcca tcaacaccat ggttatagcc ctcatgatca
      841 tactttcgct cgtctacgat tcggtgataa tggtcgacgt ggccatcgtt tacgccgccc
      901 tctccttcgt ggggaccatg ttcatcgccc gccatgtcga ggggggtgtc tagccttgga
      961 gaaagcggcc cttatcctcc tcacgatagg cgtgttgttc aacttcctcg gcacggtggc
     1021 cctttaccgc ttccccgatg tctacacccg ccttcacggg acgaccaagt gcaccacctt
     1081 cggctcgata ttcacctcgg cggcggtcgt ggtctacgcc ctccaaatgt acctgcggac
     1141 cggcgagggg cgtttcacga cgctctcggt ccacgtggtc ctggccatcc tcgccctcct
     1201 catatcgaac cccaccgggg ctcacgccat agcccgcgcg gcccacagga gcggtgtgct
     1261 tcccaagtac gccgtggtgg acaggctggc cgaggaccat cgaaagaaag gcggtgtttc
     1321 ggcatgaacg gttacgccca catggcggtc ctggccatca tcgtcatcgc ggccttttac
     1381 gccgtctggt tccgggacct tctttcatcg gtcatctcgc tggcggtctt cagcctgctg
     1441 ctggccctcg agtacttcat gctccaggcc cccgacgtgg ccattgccga agcggccatc
     1501 ggggcggggc tgagcaccgc catctacatc atcgccctca gggcgtgcaa ggccctgaag
     1561 agcccaagag ggggtgaggg gcgatgaaaa ggcaagtcct tttcgtcgtg gcagtcctcg
     1621 tcgtcgccgg ggtcttcctg ggcgccctgg ccaggataca ccccttcggg gataccacta
     1681 gggcccccat ggacgattac taccttgaga acgcccagcg ggagcgctcc gtgaacaacg
     1741 tcgtcacctc gatagtcttc gattacaggg gtttcgacac cctgggcgag gcggcggttc
     1801 ttttcacggc ggtctgctcc gtgctcgccc ttttccgtga ggggagtgag aaaagatgaa
     1861 ccccctttcc gtcgtcgtca ggaccatctg tgatattttc gcctggttcc tgggcatctt
     1921 cggcgcttac gtcatcatcc acggccacat ctcccccggc gggggtttcc agggaggggc
     1981 cgtggtcgcc accatggcgg ccctacttct cgtcgcctac ggcgggaaaa aggtcctctc
     2041 cttcgtgagg cccggcatat acaccggcat ggaggcgacg gggctgctgg ttttcataac
     2101 agctgggttc ctcggcatcg ggaccacctt cttctacaac ttcctggcgg gacagggcgg
     2161 actcttcggt tcggtggtcc ccctggggcc caacagcggc atcctcaaca gcgccggcac
     2221 catatcactg atgaacatgg ccgtgggcct ggaggtcgtc agcgggctct ccctgatatt
     2281 gatctacatg ttcaagggta tccgcctttt cgaaggccgc gagggaagga gtgagagagg
     2341 ccatgaccgg taacgccccc ttcctcgtgg tgggagtcat tttcctgctg gggctgtacg
     2401 ccatactcac cgagaggaac ctcatcaaga tagccatcgg gatatctcta ctggaggcct
     2461 cggccaacct tctcctcatc gtcctcgggt accggaccgg cgggtccgtc ccgatattca
     2521 ccctggcggg ggatgtgggg accatggtcc ttcccacccc ccaggcgctg accctcacgg
     2581 ccatcgttat cgggctggcc acatcggcgc tgctcctttc gctggtcatg ctgctttacc
     2641 gacattacgg cacgctcgac gtccgcgaga tcaggaggct gaggggatga actggcagat
     2701 gcaccttcct gcgctggccc tggccgtgcc cctccttggc gccttcctct ccctggtcgt
     2761 cggcctcggt gggcgatacc tgcgcaacgc gtggatggtt tgcgtctcca tcgccaccac
     2821 cgcgatctgc atcatgctgc tccagagagt cctcgcgggg gggacgatgg tttacgtgat
     2881 gggggccgaa tcctggaact tcacccttcc ttcgggcctc agctggccca tcaggatcat
     2941 catccaggtc gacgccttca gcgccttcat ggcggcgacc agttccatag ccgccctcgc
     3001 cggcgccttc ttctccctcc gcttcatgga caagttcacc ggcctcgaga agttctccgc
     3061 cctttacttc ctgctggtca cggggatgct gggcatgcag atcaccggtg accttttcaa
     3121 cttcttcgtg ttcctggaga tcgcttcggt ggcttccttc gggctcatcg ccttctggag
     3181 ggatcgtccc gaggcggtgg aagcctcctt caagtacatg gtggtctcca ccgtgtcggc
     3241 catgttcgtg ctcatcgccg tcgggttcct ctacggccgg tacaacgccg tcaacatagc
     3301 ggccgtcgcc aggatgctcc acatgggcta cgccgagaag atcgccctgg tcttcctcgt
     3361 cgtgtcgctg gccatgaaga gcggcgccgt ccccatgcac atgtggaccc ccgacgccta
     3421 cgccgaggcc ccggcggggg tcacctgcct gctggtcgcc gtcagccagg cgtcgctcta
     3481 cggccttttc cgggtctgct tcaccctcta cggcgtgacc gccgggagcg ttgtgattcc
     3541 ctggacgata atcatcatgg gcgtcctctc catgttcatc ggggtcacca tggcggtcat
     3601 ccagaaagag atgaaacggc tcatggccta tcattcggtc tcccagatag gctacatcct
     3661 gttggccctg ggcgtgggcc tcctctcgct caaggacccc caggccatgg ccgattacgg
     3721 tttcaccgcc atgaagggcg gcatattcca catcctcaac tacaccatgt acaagggcct
     3781 gctcttcctc tccgccggcg ccgtcttcta ctcggccgga acccgggacc tcaacgaact
     3841 gggcgggctg gccaggaaca tgccccagac gaccttcatg ttcgtcatcg ccgccgccgc
     3901 catcgcgggc cttccgccct tcaacgggtt tgtctcgaaa ctgctgatat accagacgac
     3961 cttcgccgtc tacccactgc tggccgtggc ggccctggtc acctcggtgc ttacgctggc
     4021 ctccttcgtc aaagtcttcc agaccgcttt cctcggcccc gcaaggaaga cctttgaaaa
     4081 ggtcgtggaa gtgccggcgt ccatgctttt ggggatggcc gtgctcaccc tggtcatcgt
     4141 ggtgctcacg ctcttcccgg gctggaccct ctcgcacctt gtcgaacctg ccgccaaagc
     4201 cctgatcgac cagtcaggct acatttccgc cgtcatggga ggtggtctgt aatgtgggaa
     4261 aaggtctatt cgggtttcgg ctactgggac gtgtacctct ggctcctttt cttcgctata
     4321 gcctcggccg tcatcctgtt catccggtcc aaggggcgca gcgattacaa ggaaggcacc
     4381 gagcaggatg agatattcta cggcagcaac atcgtgcccg aggacggcgc cgacatggct
     4441 gtccccgccg cctcggccta ctgggggttc gtggaggccc tcaaacctta ttacaactgg
     4501 ctgatcgaac tccacaccgg catcgccagc gaatacgtcg gctacttcat gctcaccttg
     4561 gccgtcgtag cggtgctggt ccttctttag ggggtgggaa gatgaatctg gaacgctacc
     4621 ttgacatact gacgaagtcc atctgggtct tccactgcaa cagcggctcc tgcaacggct
     4681 gcgatatcga gatcgtggcg tccataacac ccaggtatga catcgagcgc ttcggcatga
     4741 agctggtggg ttctccccgc cacgccgacg cgctgctcgt taccggcccc gtgacgaagt
     4801 acatgaccgg ccggctgcgt aggatctatg cccagatgcc cgaccccaag gtggtcatcg
     4861 ccgtggggaa ctgcgcgggc tcgggcgacg tcttctacaa gtcctacaac ctggacgg
//
LOCUS       DAOSRG010000065         4849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_15904_length_4849_cov_8.836462, whole genome shotgun sequence.
ACCESSION   DAOSRG010000065 DAOSRG010000000
VERSION     DAOSRG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15904_length_4849_cov_8.836462"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..554
                     /locus_tag="PLM30_03535"
     CDS             78..554
                     /locus_tag="PLM30_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOR54276.1"
                     /translation="MIKLFVMDVDGTMTDGAVFLDGDGGEFKRFDVRDGLGVARLTAS
                     GVRAAIISGRHSAVTERRAKELGIGIVVQGVKDKLSELTRLVAEMGLSPEEIAYIGDD
                     VNDRECIAWAGRGFAPSDARPEAREAADVVTEAPGGYGAVRDAVEDILSFNEKADK"
     gene            551..1669
                     /gene="lptG"
                     /locus_tag="PLM30_03540"
     CDS             551..1669
                     /gene="lptG"
                     /locus_tag="PLM30_03540"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOR54277.1"
                     /translation="MSGSKKKVNSVSILDRFIVTQMGNSFIFGVLVFSVLLVAGDLLF
                     QIANLMIDKGVSLGVVARLFIYKLPEVVVMTLPMASLLSALLTFGRLSSQSEIVALRA
                     AGISFRRIVRPVLAASVLVSLGAILLNETIVPLSNRAADNIMRYEIARERPTLLREKV
                     FLRDEGGGELRRIIYISRLRQNLGTMEDIVVQEFEDGTMSRIITASRGTWRGGEWWLE
                     DGKTFEVLKDRTVKLLFAFARQKLPLDLAPAEVAQTSQRPAEMSAYQLWRHISVMNKQ
                     GANLAPLKVLFHLKLAVPWACVILAVLGASLGVGHHRSGAGVGLALSVLIVFAYYVLM
                     SFSRSFGEAGYLPPIIAAWAPNLVFLIIAGYLVRIADR"
     gene            1673..2497
                     /gene="lpxI"
                     /locus_tag="PLM30_03545"
     CDS             1673..2497
                     /gene="lpxI"
                     /locus_tag="PLM30_03545"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201639.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOR54278.1"
                     /translation="MSSLEETIGLFAGEGDLSVEIARRLSEKGSPPLVFSFRKDTSPL
                     DKWALAVARVQRPAAGELLEGLRARGVKKLVFAGFVPKGLIYRPDLLDDDLRRILSSL
                     TSRDDHSLLGAVVSFFESRGITVLPYRDIIPELLAPKGVIAGRRPLPSEEEDILYGLR
                     IAGALVPLSFGQTVVVSGRSVVAVEAMEGTDETIQRAGYISGGGVVVKLMRPDQDERY
                     DVPVVGPGTLAKMHEAGLRCLAVEAGRTIVLGGERFRERAEVWEIAVTGVVPVPSS"
     gene            2452..3591
                     /locus_tag="PLM30_03550"
     CDS             2452..3591
                     /locus_tag="PLM30_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOR54279.1"
                     /translation="MGDRCDRRGPGSLFVSCGEASGDQYSKILIEALRSAGFQGVIWG
                     MLGPMGASAGGEAAWDSGALSLMGVSEVLGAVPRLVRLKGEICAEVLKRSPAGVVVID
                     SPDFHLPLLKALKKKGYRGKVFYIAPPAVWAWRSGRVSLLAELCDLCFPLFDFERIFL
                     EGHGVPCRWFGHPLSGILRGTAPADVGSPKEKTVALLPGSRKSEVSNLLPVLLETAEG
                     LAGRGYRPVFSSAPGLRVETAAMIRDRCRQWDVYDGPGTALMAASGLVIGSSGTAAVE
                     ALILGRYMIVLYRASFSSWLAYKAFVRTRWVSIPNILAGGDLYPELLQFQATPGRVLR
                     HFDDYVGDPTLRRKIDGMMMTAARSLDGGDAPRGWADSILEALPS"
     gene            3588..4688
                     /locus_tag="PLM30_03555"
     CDS             3588..4688
                     /locus_tag="PLM30_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOR54280.1"
                     /translation="MKRVLLLANGPGELWCWARPMIPALGERGFNVSLRLLPCQYASG
                     NEKDIANNLVGGGVDPPMSVFAALAGKKGIGYDAILQLGGDLLFGLAFSARNRAPLFC
                     YTYGPKPLLNRCDGVFSAFEGTCQFGGAVRDKLLIVGDLGADALAMDSGEFRWTTGRS
                     PRIVLFPGSRRVIRSAALPFIRDLAARIKERLPLSEIVIAISPFSEPGEEEKWRSLGL
                     RAVASPTGSILRGADLAVTQPGTNTLELAYSLVPGIVAVPFAFLRQVPLPGLLGLLGS
                     LPVAGPALKERVLRGRSGERGFLAWPNRLAGSEIMPEMVGDIAAIDVADRAVELLGDE
                     EGLRGIREGLMKVRQAPGAAAVIAARISELVG"
BASE COUNT          809 a         1494 c         1628 g          918 t
ORIGIN      
        1 ccatgtcagg gagatcctcg acttcatccg ctcctccaag cggggcctca cgtcctgggg
       61 cggcaagggt gaccacgatg attaagctct tcgtcatgga cgtagacggg accatgaccg
      121 acggagcggt attcctcgac ggcgacggcg gggagttcaa gcgcttcgac gtcagggacg
      181 gcctcggcgt ggccaggctc acggcttccg gggtcagggc cgcgatcatc agcggacggc
      241 attcggccgt caccgaaagg cgggcgaagg agctggggat agggatcgtg gtccagggcg
      301 tcaaggataa actctcggaa ctgacgaggc tggttgcgga gatgggcctt tcccccgaag
      361 agatagccta tatcggcgat gatgtgaacg accgggaatg catagcctgg gcgggcaggg
      421 gtttcgcccc ttccgacgcc cgccccgagg cgagagaggc cgccgatgtc gtcaccgaag
      481 cccctggagg ttacggcgcc gttcgggatg ccgtggagga catcctgtcc ttcaacgaaa
      541 aggcggataa atgagcggat ctaaaaagaa agtgaactct gtaagcatcc tggacaggtt
      601 catcgtcacc cagatgggca actccttcat tttcggggtg ctggtctttt cggtgctgct
      661 cgtcgcgggc gacctgctgt tccagatcgc caacctcatg atagacaagg gggtgtccct
      721 cggggtagtg gcccgcctct tcatctacaa actgcccgaa gtggtggtga tgaccctccc
      781 catggcttcg ctcctgtcgg cgcttttgac cttcggccgg ctctcctcgc agagcgagat
      841 cgtcgctctg cgggcggcgg ggatctcctt ccgcaggatc gtcaggccgg tcctggcggc
      901 ctcggtgctg gtctccctgg gcgccatcct cctcaacgag acgatcgtgc ccttgagcaa
      961 cagggccgcc gacaacataa tgcggtacga gatagcccgg gaacggccga cccttctgag
     1021 ggagaaggtc ttcctgaggg acgagggcgg cggtgaactg cggcggatca tttacatctc
     1081 acgcctgcgg cagaacctgg ggaccatgga ggacatcgtc gttcaggagt tcgaggatgg
     1141 cacgatgagc cgcatcatca ccgcttccag gggtacctgg aggggcgggg agtggtggct
     1201 cgaggacggc aagaccttcg aagtgctgaa ggaccggacc gtcaagctcc tcttcgcctt
     1261 cgcccggcag aagcttcccc tggaccttgc gcctgccgag gtcgcccaga catctcagag
     1321 gccggccgag atgagcgcct accaactttg gaggcatatc agcgtcatga acaagcaggg
     1381 agccaacctc gcccccctga aggtgctctt ccacctgaaa ctggccgtgc cctgggcctg
     1441 cgtcatcctg gcggttctcg gggcgtcgct gggcgtgggg caccacaggt ccggcgccgg
     1501 cgtcgggctg gccctgagcg tcctgatagt gttcgcctac tacgtgctca tgtccttctc
     1561 ccgctccttc ggcgaggccg gctacctccc ccccatcatc gcggcctggg cgccgaacct
     1621 ggtcttcctc atcatcgccg ggtacctggt gaggatcgcc gacagatagg cggtgagttc
     1681 tttggaagaa acgatcgggt tgttcgccgg cgaaggcgac ctttccgtcg agatcgcccg
     1741 tcgactctcc gagaagggga gccctcccct ggtattctcc ttcaggaagg acacttcgcc
     1801 cctggataag tgggccctgg cggtggcgag ggtccaaagg cccgccgccg gcgaacttct
     1861 cgaggggctt cgggccaggg gagtcaaaaa acttgttttt gccgggttcg tccccaaggg
     1921 gctgatctac cggcccgacc tgctggacga cgacctccgc cgcatcctct catccctcac
     1981 ctccagggac gatcactccc tgctgggtgc ggtcgtgtcc tttttcgaat cccggggcat
     2041 cacggtcttg ccctatcgtg atatcatccc ggaactgctc gcccccaagg gggtcattgc
     2101 cggcaggcgg cctctcccca gcgaggagga ggacatcctc tacggtctgc ggatagcagg
     2161 cgctctggtg cctttgtcct tcggccagac ggtggtggtc agcgggaggt ccgtcgtcgc
     2221 cgtcgaggcc atggagggca ccgatgagac tattcagagg gccgggtaca tttccggggg
     2281 cggcgtggtg gtgaaactga tgcgccccga ccaggatgaa aggtatgacg tccccgtcgt
     2341 cgggcccggg acgctggcga agatgcatga ggcgggcctg cgctgcctgg ccgtcgaggc
     2401 gggacgcacc atcgtgctgg gaggagaacg tttcagagag agggccgagg tatgggagat
     2461 cgctgtgacc ggcgtggtcc cggttccctc ttcgtaagct gcggcgaggc ttcgggggac
     2521 cagtactcga agatcctcat cgaagccctc cggtccgcgg ggttccaggg ggttatctgg
     2581 gggatgctgg gtcccatggg agcctccgcc gggggggagg cggcctggga tagcggtgcc
     2641 ctttccctga tgggtgtgtc ggaggtcctg ggggccgtcc ctcgcctggt aaggttaaaa
     2701 ggggagatct gcgccgaggt cctgaaacgg tcccccgcgg gggtcgtcgt catcgacagc
     2761 cccgacttcc acctccctct tctaaaggcc ctgaagaaaa agggataccg gggaaaggtc
     2821 ttttacatcg ccccgccggc cgtctgggcc tggcgttcgg gtagagtctc cctcctggcg
     2881 gagttgtgcg acctgtgttt cccccttttc gatttcgagc gcatttttct cgagggccat
     2941 ggcgtgcctt gccgctggtt cggacacccc ctttcgggca tcttgagggg cacggcaccg
     3001 gccgacgtgg ggtccccgaa ggaaaagacc gtggccttgc tcccggggag caggaagagc
     3061 gaggtctcga acctgcttcc cgtactactg gagaccgccg aagggcttgc cggaaggggt
     3121 taccgcccgg ttttctcctc cgcccctggg cttcgagtcg agacggcggc gatgatcagg
     3181 gaccgatgtc ggcagtggga cgtctacgac ggacccggga cggccctgat ggccgcttcg
     3241 gggctcgtga taggctccag cggcacggcg gccgtggaag ccctgatact gggcaggtac
     3301 atgatcgtgc tctaccgggc ttccttcagt tcctggctgg cctacaaggc cttcgtcagg
     3361 acgaggtggg tatccatccc caacatcctg gcgggtggtg acctttaccc ggaactcctc
     3421 cagttccagg ccaccccggg gagggtcctc aggcatttcg acgattacgt cggcgacccg
     3481 accctcagga gaaagatcga tgggatgatg atgaccgcgg cccgatccct cgacggaggc
     3541 gatgcccccc ggggttgggc ggattccatc ctggaggccc tcccgtcgtg aagagggtcc
     3601 tgctcctggc caacggcccg ggagagcttt ggtgctgggc gaggccgatg atacccgccc
     3661 tgggtgagcg cggtttcaat gtctccttga ggctcctccc ctgccagtac gcctcgggca
     3721 atgaaaagga catagcgaac aacctggtcg gcggtggggt cgacccgccg atgtccgtat
     3781 tcgccgccct cgccggaaaa aagggcatcg gctacgacgc gatcctgcag ttgggcggcg
     3841 acctgctttt cggcctggcc ttctccgcca ggaacagggc ccctcttttc tgctacacct
     3901 acggccctaa accattgctc aaccggtgcg acggggtctt ttcggctttc gaggggacgt
     3961 gccaattcgg gggggccgtc cgtgataagc tattgatcgt cggcgacctc ggcgccgatg
     4021 ccctggcgat ggacagcggc gaattccggt ggacgacggg cagaagcccg aggatcgtcc
     4081 tcttcccggg gagccgcagg gtgatccgtt ccgcggcttt gcccttcatc agggatttgg
     4141 ccgctagaat aaaagaacga ttgcccctgt cggagatcgt catcgccatc tccccctttt
     4201 ccgaacccgg agaggaggag aaatggaggt ccctgggctt gagggccgtg gcatccccca
     4261 cggggagcat cctcagggga gcggacctcg ccgtcaccca gccgggcacg aataccctgg
     4321 aactggccta cagcctggtc cccgggatag tggccgtacc cttcgctttc ctcaggcagg
     4381 tcccccttcc cgggcttttg gggctcctgg gctcgttgcc cgtcgctggc ccggccctca
     4441 aggagagggt gcttcgcgga cgaagcgggg agaggggctt cctggcatgg cccaaccgcc
     4501 tcgcgggaag cgaaattatg cccgagatgg tcggcgacat cgcggccatc gacgtggcgg
     4561 accgggcggt ggagctttta ggggacgaag aaggcctccg gggcatcagg gagggactga
     4621 tgaaggtccg ccaggccccc ggggcggccg ccgtgatagc ggcccgaata tcggaattgg
     4681 tgggatgaga aggttggcga aggaacagga gaccggcaaa agaatttacc caaggctgct
     4741 ggcccatgcc aggccctacg ttcaccgcat cctgggcgcc ctggcctgca tggtgctctc
     4801 atcggcctgc gcggtcgtcg cgccctggct cctcaggaac atcgtggac
//
LOCUS       DAOSRG010000066         4763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_16254_length_4763_cov_7.112150, whole genome shotgun sequence.
ACCESSION   DAOSRG010000066 DAOSRG010000000
VERSION     DAOSRG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16254_length_4763_cov_7.112150"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..918)
                     /locus_tag="PLM30_03560"
     CDS             complement(52..918)
                     /locus_tag="PLM30_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR54281.1"
                     /translation="MELDKILAKENFLSSGKSKDIYLIPEGRYKGKYAFVFTDRATGY
                     LDKDGKAVFDPGYDDVVGEIPGKGAIACKFAAYFFKLLAGQGVPTHFIDTVAEDVMVV
                     EPAVPIGMPEQGPEFPGSAPLLNLEWTWRHSAMGSFWRRYPFVRPCADLPVVVEAWTK
                     GKVDRLITFGSLVGAGVMTAEEVAFVEDFVKDIARVIYEEFAARGLHVLDGKVELGRL
                     KEGDGSIVLIDEISPDVLRVCNGYLEGERGCLRAEECITTSLLGEERRISAGYQLSSA
                     ELEKIFLEEERA"
     gene            1647..3233
                     /locus_tag="PLM30_03565"
     CDS             1647..3233
                     /locus_tag="PLM30_03565"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOR54282.1"
                     /translation="MGEDYVRVFDTTLRDGEQSAGINLNTAEKLQIAYNLARMNIDVI
                     EAGFPAASPGDLQAVQEIARNIKGPVIAGLARTRTGDIEAAREALRDAERPRIHTFIA
                     TSDIHLEHKLRMSREQVKEEVRRAVSLARSFVDDVEFSAEDASRSDLDYLCEVFRIAA
                     ECGATTLNIPDTVGYSLPEEFSRFVAEIIKRTDVGDNVVWSCHCHNDLGLAVANSLEA
                     VKRGVRQVECTVNGIGERAGNASMEEIVMGLRTKRSHFEVQTRVDTAGLCGLSHLVSS
                     LTGFPVPPNKAIVGANAFAHEAGIHQHGVLCNRATYEIMRPEDVGAGTKLVLGKHSGK
                     HAFRKRVADLGFDLTDDQVTQAFQLFKQLCDRKEMVTDGDLEAMIVDEFLTNTNCRRF
                     SLEEFSVQAAHGSGTAAITLSENGTKWSDAATGNGPVDAAYAALKRLTKLEPILTSYR
                     VQAVSHRSDAVGEAHITLALGKISAQGRGASTDVIEASIKAYVNAINRLHQVAASKGV
                     TIDGNDLGPGDPVQEVRRGS"
     gene            3178..4443
                     /locus_tag="PLM30_03570"
     CDS             3178..4443
                     /locus_tag="PLM30_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOR54283.1"
                     /translation="MAMTLAQAILSRKSDEAPEEGALCRVRVDLAFANDITAPPAIRS
                     FREMGAEKVFDPARCAVLPDHFTPNKDIAAAEQAKEARDFALGQGMLYWEQGRVGIEH
                     AFLPEQGHILPGEIILGADSHTCTGGALGAFATGVGSTDLAALWALGETWLRVPTTIK
                     VRFEGEKPENIGGKDLILALLSLIGVQGARYKALEFSGEVTARLPMDDRFTIANMAVE
                     TGAKAGLFAPDKTALDWVAPRAMRSFEPAFPERGAPYEKEVVVEVSGMTPLVALPHSP
                     ANVRPAREVGPMPVDQVFIGSCTNGRLSDLEQAARVLRGREVHPSVRCIVIPASREIF
                     AEALRLGFIDTFVEAGAVVCTPTCGPCLGGHMGILAKGERCVSTSNRNFVGRMGDAGS
                     EVVLSGPLVAAASAVAGRVADPSEVLAND"
     gene            4436..>4763
                     /gene="leuD"
                     /locus_tag="PLM30_03575"
     CDS             4436..>4763
                     /gene="leuD"
                     /locus_tag="PLM30_03575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162310.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOR54284.1"
                     /translation="MTRTITGKAWVFGDDVDTDVIIPARYLVTSDPKRLGEHCMEGLD
                     PDFSTKVAPGDLIVAGDNFGCGSSREHAPLALKGAGCGGVIAASFARIFFRNAINVGL
                     PIFECPE"
BASE COUNT          960 a         1454 c         1470 g          879 t
ORIGIN      
        1 cgtccttctc ctcaatggct atccatccta tggccgtttc acgcaccgag atcatgctct
       61 ctcctcctcc aggaatattt tttccagttc cgccgaggac agttggtacc cggctgatat
      121 cctgcgctct tcgcccaaaa gggacgtggt gatgcactcc tcggcccgga ggcagcccct
      181 ttccccctcc aggtacccgt tgcagacccg caggacgtcg ggcgatatct cgtctatcag
      241 cactatagag ccgtcgcctt ccttgaggcg ccccagttcg accttcccgt cgaggacgtg
      301 cagcccccgg gcggcgaact cttcgtagat gacccgggcg atatccttta cgaagtcctc
      361 cacgaaagcg acttcctccg ccgtcatcac gccggcgccc accagcgatc cgaaggttat
      421 cagcctgtcg accttcccct tggtccacgc ctcgacgacc acgggcaggt cggcgcaggg
      481 cctgacgaag gggtagcgcc gccagaagct ccccatggcg ctgtggcgcc aggtccactc
      541 caggttgagg agcggcgccg agcccgggaa ctccggcccc tgctctggca tgcctatcgg
      601 cacggcgggc tccaccacca tgacgtcctc ggccacggtg tcgatgaaat gggtcggcac
      661 cccctgcccg gccaggagct tgaaaaagta ggcggcgaat ttgcaggcta tggccccctt
      721 gcccgggatc tcgccgacga cgtcgtcgta gccggggtcg aagacggcct taccgtcctt
      781 gtccaggtag cccgtggcgc gatcggtgaa gacgaaggcg tacttgccct tgtatctccc
      841 ctcggggatc aggtagatat ccttcgattt tccggaggaa aggaaattct ctttcgccag
      901 gatcttatcc agttccatcg ctcgacctcc acggtaggta tcgaaaatca tcttaccagt
      961 ccggagagga attgaaaatc cacaaccaag agcggggtca ggtctacact tttgactaaa
     1021 tcccaagttc ggtgtcactt ttgacatact cttttgacaa agtccttttg gttgagtaac
     1081 ggagttccag gataagagat tgagcctgga atgaaagggg cacaccgaag gaacccaggc
     1141 cgggtaaggg atcctggctg aggtgggcag gagggttaag gctagaaacg tcaaaaagtt
     1201 gccaaaagtg gaaaagtcaa aagtgtagac ctgaccccaa caatgccttt gaccccaaca
     1261 atgccttgac aggtccgccc ggggaggata gaatgcccag caattagcag acgcccgata
     1321 aggcaaagga aaggaggagg cccgtgagag catcggcacc gcagggaagc aatgcgatca
     1381 tgatcgccat tcttgcgctc gcccttaccc tcggttccgg gtcccctccc gggacgccga
     1441 cgggctagtg ccgtctggcg gcattccagg gccgggaccc ttttttaagg ggcccggccc
     1501 ttttttattc tttttcccgc cggggagacg aaagaaggga ggatagcgtt acatccacaa
     1561 ggtctttccg gtgaaacact accggccttc tcatgatcag aaaaaaggcc aagctctttg
     1621 gagcgccgaa ggagagtgag atcgtaatgg gagaggatta tgtccgagta ttcgacacca
     1681 ccttgagaga cggggagcaa tcggccggga tcaacctgaa caccgccgaa aaactgcaga
     1741 tagcctacaa ccttgcccgg atgaatatcg atgtcatcga ggcgggtttc cccgccgctt
     1801 cgccgggaga cctgcaggcc gtccaggaga tagccaggaa tatcaagggt cccgtgatag
     1861 cggggctggc acggacgcga acgggggata tcgaggcggc ccgggaagcc ctcagggacg
     1921 ctgaaaggcc ccggatccac acctttatcg ccaccagcga catacacctg gagcacaaac
     1981 tgcggatgtc cagggaacag gtcaaggagg aagtgcgaag agccgtatcc ctggccagga
     2041 gttttgtcga cgacgtggaa ttttccgccg aagacgccag ccgctccgac ctggattatc
     2101 tctgcgaggt tttccgcata gccgccgaat gcggcgccac gaccctcaac attcccgata
     2161 cggtaggata ctccctgccg gaagaatttt cccgattcgt cgccgagatc atcaaaagga
     2221 ccgacgtcgg tgacaatgtc gtctggtcct gtcactgcca caacgacctc gggttggccg
     2281 tggccaattc actcgaggcg gtgaaacgag gtgtaaggca ggtggaatgc accgtcaacg
     2341 ggatagggga acgggccggt aacgcttcca tggaagagat cgtcatgggg ttacggacca
     2401 agaggtccca tttcgaggtc cagaccaggg tagataccgc cggcctctgc gggctgagcc
     2461 acttggtgtc gagcctgacg gggttcccgg tccctcccaa caaggccatt gttggagcca
     2521 acgccttcgc ccatgaagcc ggcattcatc agcacggtgt gctgtgtaac agggccacct
     2581 acgagatcat gagacctgag gatgtaggag ctgggacgaa gctggtgctg gggaagcatt
     2641 cgggaaagca cgcgttccgg aaacgggtgg ccgacctcgg cttcgacctc accgatgacc
     2701 aggtgaccca ggctttccag cttttcaagc agctctgcga ccgcaaggag atggtcaccg
     2761 atggagacct cgaggcgatg atcgtcgacg aattcttaac caataccaat tgcaggcgtt
     2821 tttcgctgga ggaattctcc gtccaggccg cccacggctc ggggacggcg gccatcactt
     2881 tatcggagaa cggaacaaaa tggagcgacg cggctacggg caacggcccc gtcgatgccg
     2941 cctacgcggc cctcaagcgg cttaccaagc tcgaaccgat ccttaccagt taccgcgtgc
     3001 aggccgtgag ccaccggtcg gacgccgtcg gcgaggccca tatcaccctg gccctgggga
     3061 aaatatcggc ccagggccgc ggagcgagta ccgacgtgat cgaagccagc atcaaggcct
     3121 acgtcaacgc tatcaaccgc ctgcaccagg tggcggcttc gaaaggggtg accatcgatg
     3181 gcaatgacct tggcccaggc gatcctgtcc aggaagtccg acgaggctcc tgaggaaggg
     3241 gccctttgcc gcgtaagggt ggacctcgct tttgccaacg atataacggc gccgccggcg
     3301 atacgctctt tcagggagat gggggcggag aaggtcttcg acccggcgcg ttgcgccgtg
     3361 ctgcccgacc atttcacccc caacaaggac atcgccgccg ccgagcaggc gaaggaagcc
     3421 cgggatttcg ccctcggaca gggcatgctc tactgggagc agggccgggt gggcatcgaa
     3481 catgctttcc tgccggaaca ggggcacatc ctgccggggg agatcatcct gggagccgac
     3541 agccacacct gcaccggcgg ggccctcggc gccttcgcca cgggagtggg cagcactgac
     3601 cttgcggccc tctgggcgct cggtgagacc tggctgcggg tgcctaccac gataaaggtc
     3661 cgattcgagg gcgaaaaacc cgaaaatatc gggggtaaag acctcatcct ggcgctcctt
     3721 tccttgatcg gcgtccaggg tgccaggtac aaggccctgg aattctccgg ggaggtgacg
     3781 gcccgcctgc ccatggatga ccgttttacc atcgccaaca tggccgtgga gaccggggcc
     3841 aaggcggggc tcttcgctcc cgacaagaca gccctggact gggtggcacc ccgtgcaatg
     3901 aggagcttcg aacccgcctt tcccgaaagg ggagccccct acgagaagga ggtggtggtg
     3961 gaagtatcgg gtatgacccc cctggtggcg ctcccccact ctccggccaa cgtccgtccc
     4021 gcccgcgagg tgggccccat gccggtggac caggtgttca tcgggtcctg caccaacggt
     4081 cgcttatcgg acctcgagca ggccgcccgg gtcctgcgtg gaagggaggt ccatccctcg
     4141 gtgcgctgca tcgtgatccc cgcttccagg gagatcttcg ctgaggccct tcgcctgggt
     4201 tttatcgaca cctttgtgga ggccggcgcc gtggtctgca cgcctacctg cggtccctgc
     4261 ctgggcgggc acatggggat cctggcgaag ggcgagcggt gcgtgtcaac cagcaacagg
     4321 aacttcgtcg gccgcatggg ggatgccggc agcgaggtcg tgctctcggg ccccctcgtc
     4381 gcggcggcga gcgccgtggc cggccgtgtg gcggacccct cggaggtgct agccaatgac
     4441 taggacgatc actgggaagg cctgggtttt cggtgatgac gtggatacgg acgtgataat
     4501 ccccgctcgc taccttgtca cgtcggaccc gaagagactc ggggaacact gtatggaggg
     4561 actggacccc gacttctcca caaaagtggc accgggcgac ttaatagtcg ccggtgataa
     4621 tttcggctgc ggctcgtcgc gggaacatgc cccgttggcc ctgaaaggtg ccgggtgcgg
     4681 cggcgtcatc gccgcttcct tcgcgcgtat ctttttccgc aacgccatca acgtggggct
     4741 tcccattttt gagtgccccg aag
//
LOCUS       DAOSRG010000067         4710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_16481_length_4710_cov_10.351880, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000067 DAOSRG010000000
VERSION     DAOSRG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16481_length_4710_cov_10.351880"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLM30_03580"
     CDS             complement(<1..513)
                     /locus_tag="PLM30_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOR54285.1"
                     /translation="MDSIEKAVRITEDMGFRVKLSRSCYESEGYHAGTDFVRADDINR
                     AFLDPEVSGIFCVRGGDGAIRLPQRLDLDAIYRNPKVFLGYSDVTILHLVLNRPRGFI
                     TFHGPMPATDFISSYFSGYVRDGLLKAVTGVEPLGEIAPCPGGPAMETLVPGTAEGEL
                     TGGNLALICAL"
     gene            503..652
                     /locus_tag="PLM30_03585"
     CDS             503..652
                     /locus_tag="PLM30_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54286.1"
                     /translation="MESIEDWLEGDTKATVSPSLRAGTFRIAGHPFTVFFYPVAVAHR
                     NKDNG"
     gene            713..1240
                     /locus_tag="PLM30_03590"
     CDS             713..1240
                     /locus_tag="PLM30_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOR54287.1"
                     /translation="MIFDPGLVQVYTGDGKGKTTAALGLAVRVCGHGGKVAFIQFMKG
                     WNFYGEIAGLSFLPGVRHERTGTPDYVYRGKESEKDRAEAARGLSLAKECLASGFYDL
                     VVLDEVNVAADYGLVDPGEAAKAIRERPPHVEVVLTGRSAPEVFLGLADLVTEMREVR
                     HPFKKGLASRRGSDF"
     gene            1357..2652
                     /gene="brnQ"
                     /locus_tag="PLM30_03595"
     CDS             1357..2652
                     /gene="brnQ"
                     /locus_tag="PLM30_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HOR54288.1"
                     /translation="MEKRSDLLSFGALAVISAALFSGHFGVGDVIFPPILGKGAGADW
                     FTAAMGYGIINSLGVLVAYLAVARQQKTLLEMSSRTLGRAFGVVFTTICMLIIGPVFI
                     LPRVSSATHEMAVALFFPDFPLVATLAVFFVLNYWVACNRAQVIDRLGKVLSPALIVF
                     MAILIIKGIVSPIGPVPATGCENPLADGVINGYNTMNALGAALFGGWVLKELHMRGIT
                     DKDAQTLNLFYIGPVVALALLVTSTGITYLGATAATVFPEADIGVYTVLIAEGVLGAV
                     GKAVFALLLAFACFTTSVGLTSTAGDVFQEMSGGKLQYKAIVAASSVVGFGIGTIGLG
                     KIVGYTVPWLMLVYPAIIVLLVGSLFDFDKFKPALQGAIVTAVILSIGDFLGAMGMAD
                     NPFSSLTAKLPLGGQGLGWLVPALVVGALAMLFAGRSKK"
     gene            2675..3553
                     /locus_tag="PLM30_03600"
     CDS             2675..3553
                     /locus_tag="PLM30_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR54289.1"
                     /translation="MQFKGELDSARVITVLDDYAGYETSFLAQHGIAILLEASSGEEC
                     KRVLLDAGQSALPILHNLELLGIDPCSIDAVVISHCHYDHTEGLVDVLKAIGRENVPV
                     VAHTTMFRPNYIFDPFIRNIGVTGKNGPAAIEEAGGLLVLVDEPFEIMPGVIVTGEVP
                     RRRAFEKQGIGTYNIEEGRVVPDEIRDDLSLAVKVRGKGLAVISGCSHAGIINIIDWS
                     REIASEPRVDLAIGGFHLIEASPERIRDTAQALKDLDVGKVVTGHCTGLAALCEFSRV
                     LGDRFEQLHVGTTIEL"
     gene            3630..4040
                     /locus_tag="PLM30_03605"
     CDS             3630..4040
                     /locus_tag="PLM30_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54290.1"
                     /translation="MKKILILSLAVLLSLPAALPASAGGCAPAAEDQERRDIVEALQA
                     ELSETLGIEAEFDFNWFGVEDGWAWVETEPRSADGLNRYEPFLALLKKEGDTWVIVDV
                     PALEEDSPPVDDAYFRGLMEAHPGLPEGIFPWGE"
     gene            4082..>4710
                     /locus_tag="PLM30_03610"
     CDS             4082..>4710
                     /locus_tag="PLM30_03610"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR54291.1"
                     /translation="MTVRDNPGEAMGRSCGLLLPVTSLPSRWGVGDLGRGAGLFLDFL
                     LSSGQGLWQVLPLSPTDPAQGHSPYSGPSAFAGNTLFISPELLVEGGLLHPSDLEGNE
                     PLEGGSCDYEAAAGLKRALFEKAFERSDLIPEEEYRRFRENNAFWLDDYALFSSLKRS
                     LGLPWYRWPAPLRDRDRGALEEAKRSLADEVKREVFLQFLFFRQWERLHG"
BASE COUNT          805 a         1513 c         1515 g          877 t
ORIGIN      
        1 cagggcgcag atgagggcga ggttcccgcc ggtcagttcg ccttcggccg tacccgggac
       61 gagggtttcc atggcggggc cgcccgggca gggagcgatc tcccccagcg gctcaacgcc
      121 ggtgaccgcc ttgaggaggc cgtcgcgaac gtagccggag aagtaactgc ttatgaaatc
      181 ggtggcgggc atggggccgt gaaaggtgat gaagccccgg ggcctgttca gcaccaggtg
      241 gaggatcgtt acgtcgctgt agcccaggaa caccttgggg ttcctgtaga tcgcgtcgag
      301 gtccagtcgc tgggggagcc taatggcgcc gtcaccaccg cggacgcaga agatgccgct
      361 gacctcgggg tcgaggaacg ccctgtttat gtcatcggcc ctgacgaagt ccgttcctgc
      421 atgataaccc tcggactcgt agcaactccg ggacagtttg acccggaacc ccatgtcttc
      481 ggtgatcctg acggcttttt cgatggaatc catcgaggac tggctcgaag gggacacgaa
      541 ggcgaccgta tcgccctccc tgagggccgg gactttccgc atcgctggac acccctttac
      601 cgtgtttttt tacccggtgg ccgtggccca ccggaacaag gataatggat agggctcccg
      661 atagcacccc cgaccgtcgg gatcgggccc gaaaacgaag ggaggatttc ctatgatatt
      721 cgatccggga ctcgtccagg tctacaccgg cgacggcaag ggcaagacca cggcggccct
      781 gggcctcgca gtgagggttt gcggccacgg cgggaaggtg gccttcatcc agttcatgaa
      841 gggctggaat ttctacgggg agatagcggg gctctccttc ttgcccggcg tccgccacga
      901 gcgcaccggc acccccgatt acgtctaccg gggcaaggag agcgagaaag accgcgccga
      961 ggccgccagg gggctgtccc tggcgaagga atgcctggcc tcgggtttct acgacctggt
     1021 cgtgctggac gaggtgaacg tcgcggccga ctacgggctc gtcgaccccg gggaggcggc
     1081 caaggcgata agggaaaggc ctccccacgt ggaggtggtc ctcaccggca ggtccgcccc
     1141 ggaggtcttc ctgggcttgg ccgacctcgt gacggagatg agggaggtca ggcacccctt
     1201 caaaaagggg ctcgcctcga ggagaggctc cgatttttga attccctgct ggtcaagggt
     1261 ttatattgga tgtagaatga tgcatctacc cttcatggta atgataatca tgttcatcta
     1321 atccgccctc gggcgaaaaa aggaggtagg tgcgttatgg aaaagcgttc ggatctattg
     1381 tccttcggag cactcgcggt tatctcggcg gcccttttct ccgggcattt cggcgtggga
     1441 gacgtgatat tcccgccgat cctgggtaag ggcgccgggg cggactggtt caccgccgcc
     1501 atggggtacg ggatcatcaa cagcctgggg gtcctcgtgg cctatctcgc cgtggcgcgg
     1561 cagcagaaga ccctcctgga gatgagctcc cgtaccctgg gaagggcctt cggcgtggta
     1621 ttcaccacga tatgcatgct tatcataggc cccgtcttca tactgccccg cgtctcctcg
     1681 gccacccacg agatggccgt ggccctcttc ttcccggact tccccctcgt ggcgacgctg
     1741 gccgtgttct tcgtcctgaa ctactgggtg gcctgcaaca gggcccaggt catcgaccgg
     1801 ctcggcaagg tcctttcccc ggcactgata gtcttcatgg ctattctcat cataaagggg
     1861 atcgtctcgc cgatcgggcc cgtaccggcg acgggttgtg agaaccctct cgccgacggg
     1921 gtcatcaacg gctacaacac catgaacgcc ctcggggccg ccctgttcgg gggatgggtc
     1981 ctcaaggaac tgcacatgag gggcatcacc gacaaggacg cccagacgct gaacctcttc
     2041 tacatcggcc ccgtcgtcgc cctggcgctc ctcgtcacct ccacgggcat cacctacctg
     2101 ggcgcgacgg ccgccacggt cttccctgaa gccgatatag gcgtctacac cgtcctgatc
     2161 gctgagggag tgctcggggc cgtgggcaag gccgtcttcg cgctcctcct ggccttcgcc
     2221 tgcttcacca cctcggtggg tctcacctcc accgccggcg acgtcttcca ggagatgtcc
     2281 ggcggtaaac tgcagtacaa ggccatcgtc gcggcctcca gcgtcgtggg cttcgggatc
     2341 gggaccatcg gcctcggcaa gatcgtcggc tacaccgtgc cctggcttat gctggtctat
     2401 ccggctatca tagtgctgct ggtggggagc cttttcgact tcgacaagtt caagcccgcc
     2461 ctccaggggg ccattgtcac ggcggtcatc ctcagcatcg gcgatttcct cggcgccatg
     2521 ggcatggccg acaacccctt cagctccctg acggcgaagc tccccctcgg ggggcagggc
     2581 ctggggtggc tggtaccggc cctcgtcgtc ggcgccctgg cgatgctttt cgccggccgg
     2641 tccaaaaaat agcggcccgg gaggttggag cgatatgcaa ttcaagggtg aactggattc
     2701 agcgcgggtc atcacggtcc tcgacgacta cgccgggtac gagacgtcct tcttggccca
     2761 gcacggcata gccatcctgc tggaggcctc ttcgggagag gagtgcaagc gggtcctgct
     2821 cgacgcgggg cagtccgccc tcccgatact gcacaacctg gaactcctgg gcatcgaccc
     2881 ctgttcgatc gacgccgttg tgatctccca ctgccattac gaccacaccg aggggctcgt
     2941 ggacgtcctc aaggccatcg gccgggaaaa cgtccccgtg gtggcccaca cgacgatgtt
     3001 ccggcccaat tacatcttcg accccttcat ccgcaacatc ggcgtcaccg ggaagaacgg
     3061 ccccgccgcc atagaggagg cgggggggtt gctggtcctc gtcgacgagc ccttcgagat
     3121 catgcccggc gtcatcgtga ccggcgaggt cccccgaagg agggccttcg agaagcaggg
     3181 gatcgggacc tacaacatcg aagaggggag ggtcgtcccc gacgagatcc gcgacgacct
     3241 ctccctggcc gtgaaggtcc gggggaaggg cctggccgtc atctccggct gcagccacgc
     3301 cgggataata aacatcatcg actggtccag ggagatagcg tcggagccca gggttgacct
     3361 ggccatcggc ggcttccacc tgatcgaggc ctcaccggaa aggatcaggg ataccgccca
     3421 ggccttgaag gacctcgacg tgggcaaggt cgtcacgggc cactgcacgg gcctggccgc
     3481 cctgtgcgag ttttccaggg tgctggggga ccggttcgaa cagctccacg tcgggaccac
     3541 gatagagctt taagcctatc ggctgttccc aaggcggcgc ccgtcccgga gcgccgcctt
     3601 tttttcaaga tgaaaggggc tgagagggga tgaaaaagat cctgatcctt tcattggcgg
     3661 ttttgttgtc gctcccggcg gcattgccgg cctccgccgg cgggtgcgcc cccgcggccg
     3721 aagaccagga gcgaagggac atcgtcgagg ccctccaggc ggaactgtcc gagaccctcg
     3781 gcatcgaggc cgaattcgac ttcaactggt tcggcgtcga ggacgggtgg gcctgggtcg
     3841 agacggaacc gcggtcggcg gacggcctga accgttacga gcccttcctc gcgctcctga
     3901 aaaaggaggg cgatacctgg gtcatcgtcg atgtgcccgc cctggaggag gactcccctc
     3961 ccgtcgacga cgcctacttc agggggctca tggaggccca ccccggcctg cccgaaggga
     4021 tcttcccctg gggtgaataa taccccgccg tggcgggcgg ccctccacgg gaggccgacc
     4081 gatgacggtc cgggataacc cgggtgaagc aatgggcagg agctgcggcc tcctcctccc
     4141 cgtgacctcc cttccatcca ggtggggcgt cggcgacctg gggagggggg ccggcctttt
     4201 cctggacttc cttctctcct cgggccaggg cctttggcag gtcctccccc tctcgcccac
     4261 cgaccccgcc cagggccact ctccctacag cggcccttcg gccttcgctg ggaatacact
     4321 tttcatcagc ccggaactcc tcgtcgaggg gggccttctc catccttcgg acctcgaagg
     4381 caacgagccc ctggagggcg gctcttgcga ttacgaggcc gccgccggtc ttaagcgggc
     4441 ccttttcgaa aaggcctttg agaggtccga cctcatcccc gaggaggagt accggcgttt
     4501 tcgcgaaaac aacgccttct ggttggacga ctacgcgctc ttttcatcct tgaagaggtc
     4561 cctggggctc ccctggtacc ggtggcccgc accgctgcgc gacagggaca ggggcgccct
     4621 tgaggaggcg aagaggagcc tggccgacga ggtgaaaagg gaggtcttcc tccagttcct
     4681 cttcttccgc cagtgggagc gcctccacgg
//
LOCUS       DAOSRG010000068         4703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_16522_length_4703_cov_7.574225, whole genome shotgun sequence.
ACCESSION   DAOSRG010000068 DAOSRG010000000
VERSION     DAOSRG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16522_length_4703_cov_7.574225"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLM30_03615"
     CDS             complement(<1..394)
                     /locus_tag="PLM30_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOR54292.1"
                     /translation="MRPAVVAKILSLLCAIVSLFMAFPVLWSLADGSSDLFPLLAGMG
                     TGLGLSGLLFAAGFKAKAGDLGVREAFAVVSLSWVLASAIGGIPYLLYGTVPTYTEAF
                     FEAMSGFTTTGATVLTDIQSNPRGILFWR"
     gene            complement(396..1760)
                     /gene="trkA"
                     /locus_tag="PLM30_03620"
     CDS             complement(396..1760)
                     /gene="trkA"
                     /locus_tag="PLM30_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOR54293.1"
                     /translation="MKIVVVGAGEVGFSVASSLSQEGHDVTVVESDEERAAKAENELD
                     VMVVRGNGSRPPVLEQAGICPGCDTDLLIACSNHDEVNILACWIARRAGVKRVISRAR
                     GLEYTDSPTWARQLGIDVMISPERSVAREIEEFLSVSAAVYTAEFFGGKVGVYAFRVS
                     PDSALMGASLRDLRRIHPKKTALIVFIERQGKGFIPSGDDSLEADDLCFTVTSKSQAQ
                     DMEVLFTNKKTPELKRVIIVGGGKIGFQVATRLGLRYPGLDLRLIDEDREKCEKLSRE
                     LDKAIIIHGDGMDEELLRNEGIDEADGFVTTTANDEHNLLIGVVGKALGAKKSVAVVR
                     REMLMRLTDYIAVDAMVNPNQALATVIMKQVRYPFGTGALSIIDEIDAEVLELTIPHG
                     SAAGGKKIMDLGLPKGILIALVSRGTEMFVPWGNSVLRENDEVLIFSSEELMNKAVEI
                     LGVG"
     gene            1848..2837
                     /locus_tag="PLM30_03625"
     CDS             1848..2837
                     /locus_tag="PLM30_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOR54294.1"
                     /translation="MPRREKIAFVGTGGTVSMTFNENQNGFIPTLSAGDLAGMLPPGL
                     RNDLDVIDWSHQPSSHYTIPMTTDLVELLRKHVKDGASGIVVTCGTDSLEEMAYLTDL
                     LWAYPQPVIFTGSMLPPGLQGSDALANLAQAVKAASSENLWGVGVLACFQDQLFAASE
                     VTKEVAHRRDAFAAPGRGPVADFVGGDIRVIRKPNRPPALEGAIMPARNVEILWSSLG
                     GGDGTLSCLSKSKNLDGLVLAGFGAGNIPPSWIQYLKPIMKNGTVIVITTRCRRGHTA
                     TLYGYEGSTKRLLEMGLLDGGGLRPEQARLRLAVGLGAGFSREDLQLYLLGKK"
     gene            complement(2864..>3164)
                     /locus_tag="PLM30_03630"
     CDS             complement(2864..>3164)
                     /locus_tag="PLM30_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOR54295.1"
                     /translation="GVGFFLIWALVATAGKDLLRANALKASIALCFTVVSFVIFFSRG
                     LINMEYGLALAVGSMIGAWMGTRFSVVKGNKWIRYVLVVMVVISALRMIFQVLGS"
     assembly_gap    3165..3264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3265..3771)
                     /locus_tag="PLM30_03635"
     CDS             complement(<3265..3771)
                     /locus_tag="PLM30_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOR54296.1"
                     /translation="MTAAIMVLATLLGGVTGFINVVAGGGSLLSLPFLIFLGLDASSA
                     NATNRVSILLQNLVATGQFKKEGVLSLRESLSLGIPASIGSVAGTLLAVQLDERFLKM
                     TIALLISSMAVLLIARPRMWESCRVTLWPAWAKWPLFLVIGAYGGFLQAGVGFFLIWA
                     LVATAGKDL"
     gene            3889..4311
                     /locus_tag="PLM30_03640"
     CDS             3889..4311
                     /locus_tag="PLM30_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOR54297.1"
                     /translation="MRYAITEDEAFLRLSDGEELHLSIAAACEEYSIDSAIILGGLGM
                     AREITFGWYTGKEYLRERVEGTFEVASLSGDVSRREGALYPHIHAVFNGMDHASLGGH
                     VLKVVAFHNMEVFIRPLRSIRLNREYDGWLEAIVPEKR"
     gene            complement(4308..>4703)
                     /locus_tag="PLM30_03645"
     CDS             complement(4308..>4703)
                     /locus_tag="PLM30_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOR54298.1"
                     /translation="TIRTVEDASRSVGAPPEEILKTLVLLADDKPVIALMSGPNRIDL
                     KKVKALLKARKVSLAKAEWVLDHLGFEVGGVPPVGFPTMPCALVDEDLFRFETVWAAA
                     GTDHSFFPVNPGQLTGYIGGVKADLKKES"
BASE COUNT          931 a         1485 c         1363 g          824 t
ORIGIN      
        1 cgcgccagaa gaggatgccc ctggggttgg actggatatc ggtgagcacc gtggctcccg
       61 tcgtagtaaa gcccgacatg gcctcgaaga acgcctcggt gtaggttggg actgtcccgt
      121 acagcaggta ggggatgccc ccgatggccg aggccaggac ccacgagagg gagaccacgg
      181 cgaaggcttc cctgacgccc aggtccccgg ccttcgcctt gaacccggca gcgaaaagga
      241 gccccgagag ccccagaccg gtacccatcc ccgccagcag agggaaaagg tcactggacc
      301 cgtccgccag ggaccagagg accgggaagg ccatgaagag cgagacgatg gcgcagagta
      361 gggagagtat cttggcgacg acggcgggtc gcaccctacc ctacccccag tatctccacg
      421 gccttattca tcagttcctc cgaagaaaag atgaggacct cgtcgttctc ccggagcacg
      481 ctgttgcccc aaggcacgaa catctccgtc cccctcgaga cgagggcgat gagtatcccc
      541 ttcgggagcc ccaggtccat gatcttcttc ccgccagccg cgctcccgtg ggggatcgtc
      601 agttccagga cctcggcgtc gatctcatcg attatggaga gggccccggt gccgaaggga
      661 tagcgcacct gcttcattat caccgtggcc aaggcctggt tggggttgac catggcatcg
      721 acggcgatgt agtccgtcag gcgcatgagc atttcccgcc tcaccaccgc cacgcttttc
      781 ttcgccccca aggccttccc gaccacgccg atcaggaggt tgtgttcgtc gttcgccgtg
      841 gtcgttacga aaccgtcggc ttcgtcgatc ccctcgttcc tcaggagctc ctcgtccatt
      901 ccgtcgccgt gaattattat cgccttgtcg agctcccggg ataacttctc gcacttctca
      961 cggtcctcgt cgatgagcct caggtcgagc cctgggtacc tcaaccccag cctggtggcc
     1021 acctggaagc ctatcttgcc gccgccgacg atgatgaccc ttttcaattc gggggtcttc
     1081 ttgttcgtga agagaacctc catgtcctgg gcctgggatt ttgatgtgac ggtgaagcag
     1141 aggtcatcag cctcgaggct gtcgtcgccg gagggaatga agcccttacc ctgcctttct
     1201 atgaacacga tgagggccgt cttcttgggg tggatacggc gcaggtccct cagggaagcc
     1261 cccatgaggg cggaatcggg actgactcga aaggcgtaca ctcctacctt gcccccgaag
     1321 aactccgcgg tgtacacggc tgcgctcacg gagaggaact cctcgatctc ccttgcgacg
     1381 gagcgctccg gggagatcat cacgtctatc cccaattgcc ttgcccaggt cgggctgtcg
     1441 gtgtactcca agcccctggc cctggatatg accctcttga cgccggcccg acgggcgatc
     1501 cagcaggcaa gtatgttgac ttcgtcatgg ttcgagcatg ctatgaggag atccgtgtcg
     1561 cagcctgggc agatcccggc ttgttcgagc acgggcggcc ttgagccgtt ccccctgacg
     1621 accatgacgt ccagttcgtt ctcggccttg gcggcccttt cctcatcgct ctccacgacg
     1681 gtgacgtcat gcccttcctg cgaaaggctg gaagcgacgc tgaacccgac ctcacccgcc
     1741 ccgaccacca cgatcttcaa acctgataca ccccccggga tgggacaacc ccgacttcgc
     1801 ctgccaagcg aaagtggtaa actagtccgg acaagggagg tggacccatg ccgcgccgtg
     1861 agaagatcgc cttcgttggc acaggcggga ccgtcagcat gaccttcaac gagaatcaga
     1921 acggtttcat tcccactctc tccgccgggg acctggcggg gatgctcccc ccgggcctca
     1981 ggaacgacct cgacgtgatc gactggagcc accagcccag cagtcactat accataccga
     2041 tgaccaccga tctcgtggaa cttctgcgca aacacgtgaa ggacggggcc tccgggatag
     2101 tcgtgacttg cgggaccgac tccctcgagg agatggccta cctgaccgat ctcctctggg
     2161 cctaccccca gccggttatc ttcacgggat ccatgctgcc gccgggtctt cagggatcgg
     2221 acgccttggc aaacctcgcc caggccgtga aagcggcctc ttcggaaaac ctttggggcg
     2281 tgggcgtcct cgcttgcttc caggaccagc ttttcgccgc ctccgaagtc accaaggagg
     2341 tggcccaccg cagggatgcc ttcgcagcgc cgggaagagg acccgtggcc gacttcgtag
     2401 gcggggacat aagagtcatc agaaaaccta acaggcctcc cgccctggaa ggggcgatca
     2461 tgcccgccag gaacgtggag atcctctggt cgtccctcgg gggcggcgac gggaccctct
     2521 catgcctttc caagtcaaaa aacctcgatg gacttgtcct ggctggtttc ggcgccggta
     2581 acataccccc ctcatggatc caatacctca aacccatcat gaagaacggc acggtgatag
     2641 tgataaccac gcgctgcagg agggggcaca ctgccaccct ctatggttac gagggaagca
     2701 ctaaaagact gctcgaaatg ggccttcttg acgggggcgg cctgaggccg gagcaggcca
     2761 ggctgaggct cgccgtaggg cttggagcgg gtttttcaag ggaggatctg cagctttacc
     2821 tcctgggcaa aaaatgaagg cccctcggga aaggtccgcc gcctcaagac ccgagcacct
     2881 ggaagatcat cctcagggcg ctgatcacca ccattaccac aaggacgtac cttatccact
     2941 tgttaccctt caccacggaa aaccgcgtcc ccatccaggc cccgatcatg ctgccgacgg
     3001 cgagggccaa gccgtactcc atattgatga gcccccgcga gaaaaaaatg acgaagctga
     3061 ccacggtgaa gcagagcgcg atggaagcct tgagcgcatt ggccctcaag aggtccttgc
     3121 ccgctgtggc caccagggcc cagatcagga agaagcccac tcccnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnngaggtc cttgcccgct gtggccacca gggcccagat
     3301 caggaagaag cccactcccg cttgtaaaaa ccccccatag gcgcctatga ccaggaaaag
     3361 aggccacttg gcccaggcag gccataaagt gaccctgcag ctctcccaca tcctcggcct
     3421 ggcgatgagg aggaccgcca tggaggagat caagagggcg atggtcatct tcagaaagcg
     3481 ttcatccagc tgcacggcga gaagggtccc cgccaccgac ccgatggagg ccggtattcc
     3541 cagggaaaga gactccctca gggacagcac gccttccttc ttgaactgcc ccgtggcgac
     3601 caggttctgg agcaggatgc tcacccggtt ggtggcattg gccgaagagg cgtccaggcc
     3661 gagaaagatc aggaaaggaa ggctgaggag ggacccaccc ccggccacga cattgatgaa
     3721 accggtcact cctccgagca gggtcgcgag aaccattatc gctgcagtca aaccagacgc
     3781 ctccatggga actcagatcg caaccaggcg tcattctagc atacaacggc cgggcggcag
     3841 cgctctggag tgatataatc aattaacgca agaagggagg ggaaacccat gcgctacgcc
     3901 atcaccgagg acgaggcctt tctccgtctg agcgacggcg aagagctaca cctctcgata
     3961 gcggcggcct gcgaggagta ctccatcgac tcggccatca tactgggcgg actgggtatg
     4021 gcccgagaga taaccttcgg ctggtacacc gggaaggagt acctccggga gcgggtggag
     4081 gggaccttcg aggtcgcgtc gctgagcggg gacgtcagcc gaagggaggg ggccctctac
     4141 ccgcacatcc acgccgtctt caacggaatg gaccacgcct cgctgggcgg gcacgtcctg
     4201 aaggtcgtcg ccttccacaa catggaggtc ttcatccgcc cccttaggtc gatccgcctc
     4261 aaccgggaat acgacggctg gctggaggct atagtgcctg agaagcgcta gctttccttc
     4321 tttaggtccg ccttgactcc cccgatgtag ccggtcagct gccccgggtt gacggggaag
     4381 aagctgtggt cggtgccagc ggccgcccat accgtctcga accggaaaag gtcctcatcg
     4441 accagggcgc agggcattgt cgggaacccc accggcggaa cgccgcccac ctcgaacccc
     4501 aggtggtcca ggacccactc ggccttcgcc aaggacacct tcctggcctt gagaagggcc
     4561 ttcacttttt taaggtctat cctgttaggc cccgacatca gggctatgac gggtttgtcg
     4621 tcggccagga ggacgagagt cttcaggatc tcctccgggg gagcgcccac gctcctggag
     4681 gcgtcctcca cggtccttat ggt
//
LOCUS       DAOSRG010000069         4702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_16526_length_4702_cov_7.169572, whole genome shotgun sequence.
ACCESSION   DAOSRG010000069 DAOSRG010000000
VERSION     DAOSRG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_4702_cov_7.169572"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLM30_03650"
     CDS             complement(<1..283)
                     /locus_tag="PLM30_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HOR54299.1"
                     /translation="MRVITEVAGEAQGLVPVEVLGRIPGALGPVAEEIFRFLCPEWGP
                     SGDLFLSVNFIDEGQMSELNHRFMGEGHPTDVLSFPIHASGGKFTCPAPL"
     gene            complement(280..2337)
                     /locus_tag="PLM30_03655"
     CDS             complement(280..2337)
                     /locus_tag="PLM30_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR54300.1"
                     /translation="MNKSSDKGPAPSQQRLTAGNPAGDKGYRPHWAAAVMLAAVASIL
                     LAGAWHFKEGESSFRVGSPAMKTYYAHSRMRIVDESATRALREQRMSDIGGVLVKDRK
                     FPEEVRGKFAALEKRDYAAVLPDSLSRIMESLPEDAARRITGVASRIALSFQDSLSVG
                     AELPADMLWRSLETSGLSIPEQNLAYQILDHVMERALVMDSEVTQEVRSLAASEVEPV
                     ERVLSVGDIIVREGEVISPEVALVLRAQGYPEAFFPWKTLAFIVTGVFFWVSWMGWFS
                     RRREYHFDARESAFMITILIAGWVAMSLSSLLGGNGLGVIPLAGWAFLALPGSFAFQL
                     VLWGGIIGSVITSGSSSAGLMLSVFTSGLTASLAYLLLRKITDRLDLLRKMALLGVSL
                     ALGGFFIGWGLYLPSGGTALLSLSASALAWIVVTLAALPLWERLFDILTPVRLVDLTH
                     PSHPLLKRLQLEAPGTYHHSLMVGTLAEAAAEKLRMDPLLVKAGAYFHDVGKLRRPQF
                     FVENQQTGLNPHDELAPSLSALIIISHVRDGLEMARQFGVPKKIRVFIGEHHGTTCLH
                     YFFKKALGADPDLPMEQFCYPGGRPSSRESAVLMLADSVEAAVRAAAPAITDSRELEE
                     MTKEVIDTKVAESQLSDVPLTFQDLTRIKAAFIETLKHMYHARKVTPLENAGDTPREP
                     AVS"
     gene            complement(2318..3328)
                     /locus_tag="PLM30_03660"
     CDS             complement(2318..3328)
                     /locus_tag="PLM30_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOR54301.1"
                     /translation="MRPVRGEATAAERENHGHTGSVEAADEETFSRLLGPRDENLRLI
                     EERYPVRLVVRDLLISVHGPDADAVDLVMELLRQMMKIARKGHALRPAEVTYAMNAFA
                     EQGVIDLMPLYEDVICLTHRGKPVRPKTEGQKRYVDAIRKNDVLFAIGPAGTGKTYLA
                     VGLAVAALKEGKVNRVVLVRPAVEAGESLGYLPGDLREKVEPYLRPLYDAFYEMFSPE
                     RFTKYVEKKIIEIAPLAYMRGRTLNDSFIILDEAQNTTPEQMKMFLTRLGFGSKAVVI
                     GDITQIDLPSGKKSGLKVVRNILKGIPGVEFVSLGKNDVVRHEVVQRIVQAYEEHEQK
                     QR"
     gene            complement(3325..3561)
                     /locus_tag="PLM30_03665"
     CDS             complement(3325..3561)
                     /locus_tag="PLM30_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOR54302.1"
                     /translation="MEIEERVKSLIEEQVRPALQSHGGDIELVSFDTDGGVVTVRLKG
                     ACGNCPFAAETLRSQVEAVIRKEIPEIEKVVRTG"
     gene            complement(3604..>4702)
                     /locus_tag="PLM30_03670"
     CDS             complement(3604..>4702)
                     /locus_tag="PLM30_03670"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOR54303.1"
                     /translation="REFVKRAVGNGLDIIRVFDALNDLRNMEVAADQVKKEGAHLQMS
                     LSYTISPMHTLEAFADMSLKMKKMGADSICIKDMAGLLGPVQAQKLVKAVKRKTSLPV
                     QVHSHYTSGMAAMAYYASVLAGADVVDCAISPFAMGTSQPATETMVAALTGKDFDTGI
                     TLDRLVPVSEYFKELREKYSKLIMGLEGVDINVLVYQIPGGMYSNLVSQLKEQDSLHR
                     LQDVLREVPKVRKEMGYPPLVTPTSQLVGTQATLNVLMGERWKMVSKEVRQYFLGYYG
                     TPPGPLDKEVQKKVIGEERPITCRPGEVLKPELPGVYKTMGDWITQPEDALSYALFPA
                     VAKDFLVRKFARENLRDVGLQPLVEGAAYPL"
BASE COUNT          905 a         1440 c         1380 g          977 t
ORIGIN      
        1 gaaggggggc cgggcaggta aacttccctc cggaggcatg gatcggaaag gacaggacgt
       61 ccgtgggatg cccttcaccc atgaagcggt ggttgagctc gctcatctgg ccctcgtcga
      121 tgaaattaac cgagagaaaa agatcccccg aggggcccca ctccggacaa aggaaccgga
      181 agatttcctc cgcgacaggc cccaacgctc cgggtatcct gccaaggacc tcgacgggca
      241 ccaggccttg ggcttccccg gctacctccg tgatgaccct catgacacgg cgggctcccg
      301 aggcgtatcc cccgcatttt ccagcggggt taccttcctg gcgtgataca tgtgcttgag
      361 ggtctcgatg aaggctgcct ttatcctcgt aaggtcctgg aatgtgagag gtacatcgga
      421 tagctgggat tcagccacct tcgtgtctat gacctccttc gtcatttctt ccagttcgcg
      481 gctgtcggtt atggccgggg cggccgccct gacggcggcc tcgaccgaat cggcgagcat
      541 gaggaccgcc gattcccggc tggacgggcg gcctccgggg tagcagaatt gctccatggg
      601 caggtcgggg tcggcgccca gggccttctt gaaaaaataa tgaagacagg tagtcccatg
      661 atgttccccg atgaaaaccc tgatcttttt cggaacaccg aactggcggg ccatctcaag
      721 cccatcccgc acatgggata ttattataag cgccgagagg gaaggtgcca gttcgtcgtg
      781 ggggttgagt cccgtctgct ggttctcgac aaagaactgg ggcctcctga gcttccctac
      841 gtcatgaaag tatgcccccg ccttgaccaa gagggggtcc attctcagct tctccgccgc
      901 ggcttcagcc agcgtgccga ccatcagtga gtggtgataa gttccgggag cttccagctg
      961 gagccttttc agcagcgggt gtgaagggtg ggtcaagtcc acgaggcgga cgggcgtgag
     1021 gatatcaaag agcctctccc ataaagggag cgctgccaag gtcaccacga tccaggccag
     1081 ggccgaggcc gaaagggata gaagggccgt gccccccgag ggaaggtaga gaccccatcc
     1141 aatgaagaac cctccgagcg cgagggaaac ccccaggagg gccatcttgc gaaggaggtc
     1201 caggcggtcc gtgatcttcc tgagaagaag gtaagccagg ctggccgtta aacccgaggt
     1261 gaacaccgaa agcatcaatc ccgccgatga actgcccgaa gtgatcaccg acccgatgat
     1321 ccccccccag aggacgagtt gaaacgcaaa ggaccccggt agggccagga aagcccagcc
     1381 cgccaggggg atgaccccca gcccattccc accgagcagg gacgataggg acatggccac
     1441 ccagccggcg atgaggatcg tgatcatgaa agccgattcc cgggcgtcga aatggtactc
     1501 ccggcgcctc gagaaccacc ccatccagga aacccagaaa aagacccccg tcacgatgaa
     1561 ggccagggtc ttccagggaa agaaagcctc ggggtatccc tgcgctcgaa ggacgagagc
     1621 gacctcgggc gagatcacct caccctccct gacgatgatg tcccccaccg acagcaccct
     1681 ttcgacaggt tcgacctcgg aagccgcgag ggacctcact tcctgggtca cttcggagtc
     1741 catgaccagg gctctttcca tgacgtgatc caggatctgg taggcgaggt tctgttccgg
     1801 gatggaaagg cccgaggtct caaggctcct ccacagcatg tcagccggga gttccgctcc
     1861 caccgagagg gagtcctgga aagacagggc gatcctagaa gcgacgccgg ttatcctccg
     1921 ggccgcgtcc tcaggaaggc tttccataat cctggacagg gaatcgggca gaacggcggc
     1981 ataatccctc ttttccaagg cggcgaactt gccccggacc tcttcgggga acttcctgtc
     2041 cttcaccagg acgcccccga tatcggacat tctttgttcc ctcagggccc ttgtggcgct
     2101 ctcatcgact atcctcatgc gggaatgggc atagtaggtt ttcatggcag ggctgcccac
     2161 cctgaaagag ctctcccctt ccttgaagtg ccacgcgccg gcgaggagta ttgaagcgac
     2221 ggcagccagc atgacggcag cggcccagtg agggcggtat cccttgtcgc cggccgggtt
     2281 gcctgcggtg agtctttgtt gtgatggggc gggaccttta tcgctgcttt tgttcatgtt
     2341 cttcataggc ctggacgatc ctctggacga cttcgtggcg gacgacgtcg tttttgccca
     2401 gggacacgaa ctcgaccccg gggatcccct tgagaatatt cctgacgacc ttgagacccg
     2461 acttcttccc cgatggaagg tcgatctggg ttatatcgcc gatgacgacc gccttcgacc
     2521 cgaaaccgag cctggtgagg aacattttca tctgttccgg cgtggtgttt tgggcctcgt
     2581 cgagtattat gaagctgtcg ttcagggtcc tgcccctcat gtaggccagg ggggcaatct
     2641 ctatgatctt cttctcgacg tacttcgtga acctctccgg ggagaacatt tcgtaaaagg
     2701 catcgtaaag aggccttagg tagggctcca ccttctccct gagatccccc gggaggtaac
     2761 cgaggctttc gccggcttcc acggcgggtc ggaccaggac gaccctgttg accttgccct
     2821 ccttgagggc ggccacggca agccccactg ccaggtacgt cttgcccgtt cccgcggggc
     2881 ctatggcgaa gaggacgtca ttcttgcgta tggcgtcgac atagcgtttc tgcccctccg
     2941 ttttcggacg taccggcttt cccctgtggg tcaggcatat cacgtcctcg tacaggggca
     3001 taagatcgat gacaccctgc tcggcgaagg cgttcatggc gtaggtgact tcggcgggcc
     3061 ggagagcgtg gccctttcgg gcgatcttca tcatttgccg gagcagttcc atgacaaggt
     3121 ccaccgcatc ggcatcgggg ccatggacgg agatgaggag gtcccggacg acgagcctga
     3181 cgggatagcg ctcctcgatg agccttaggt tctcgtccct aggacccagt agtcgtgaaa
     3241 aagtctcttc atccgcggcc tccacggaac cggtgtggcc gtggttctct ctttcggccg
     3301 ccgtcgcctc gcctcttacg ggtctcatcc cgtcctgacg accttttcta tctcaggtat
     3361 ctcctttctg atgacggctt ccacctggga acgcaacgtc tcggcggcga aggggcagtt
     3421 gccgcacgcc cccttcagcc ttacggtcac gaccccgccg tcggtgtcaa aactcacgag
     3481 ttcgatatcc cccccgtggg actggagagc cggtctgacc tgttcttcga tgagcgactt
     3541 cactctttct tctatttcca tgaaaagcac ctccgatcat cctgatgctg acaatgatcc
     3601 gatctaaagc gggtaagccg cgccttccac cagaggctga aggcccacgt ccctgaggtt
     3661 ttccctggca aacttcctta caaggaaatc cttcgcgacg gccgggaaga gggcgtaact
     3721 cagcgcgtcc tcgggctgtg ttatccaatc ccccatggtc ttgtagacac caggcaattc
     3781 tggtttcagg acctcccccg gccggcaggt gatcggcctc tcttcaccga tgaccttctt
     3841 ctggacctcc ttgtccagcg gtccgggggg cgttccgtag taccccagga agtactgcct
     3901 cacttccttg gaaaccatct tccacctctc gcccatgaga acgttgagcg ttgcctgggt
     3961 gccgacgagc tggctcgtag gtgtgaccag tggcgggtag cccatctcct tgcggacttt
     4021 ggggacctcc cggagcacat cctggagcct gtggagagaa tcctgctcct tgagctggct
     4081 cacaaggttg gagtacatcc cgccggggat ctggtagacg aggacgttga tgtcgacccc
     4141 ttcaagaccc atgataagct tgctgtactt ctcccggagt tccttgaagt attccgatac
     4201 aggtacgagc ctatcgaggg ttattcccgt atcgaaatcc tttcccgtga gggccgccac
     4261 catcgtctcc gtggcaggct ggctggtccc catcgcgaag ggcgagatcg cgcagtccac
     4321 cacatcggcc ccggccagca ccgaggcgta ataagccatg gcggccatgc cactggtgta
     4381 gtgggaatgg acctggaccg gcagggacgt cttccttttc accgccttca ccagtttttg
     4441 ggcctggacc ggcccgagga gtcccgccat gtcctttatg caaatggaat cggcgcccat
     4501 cttcttcatc ttgagggaca tatccgcgaa ggcctccagg gtgtgcatgg gggatatcgt
     4561 gtaagagagg ctcatctgga ggtgggcccc ttcctttttg acctggtcgg cggcgacctc
     4621 catgttcctg aggtcattca aggcgtcgaa aaccctgatt atgtcgaggc cgttgccgac
     4681 ggcccttttg acgaactccc tc
//
LOCUS       DAOSRG010000070         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_16988_length_4602_cov_8.425555, whole genome shotgun sequence.
ACCESSION   DAOSRG010000070 DAOSRG010000000
VERSION     DAOSRG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16988_length_4602_cov_8.425555"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..639)
                     /locus_tag="PLM30_03675"
     CDS             complement(34..639)
                     /locus_tag="PLM30_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOR54304.1"
                     /translation="MLEEINFVALIPYVFVTTFTPGPNNISCAAAAARWGMRRTWGYL
                     LGIGMGFFVVLLLAGAFSGALISALPALEGYLRWVGAAYILWLAYGIARSEGQGGMGG
                     GEPMRGFAKGFVLQFANPKSILFSITLYTAFLGPILARPTPVFLSAALVAAIGFTSIM
                     AWAFFGLGIHRFLQNPLHHRIINLGFSLLLVWTAVGVAGIL"
     gene            complement(699..1703)
                     /locus_tag="PLM30_03680"
     CDS             complement(699..1703)
                     /locus_tag="PLM30_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HOR54305.1"
                     /translation="MELTRATAVVDTHTGGEPTRIIIGGGPYIPGKTMSERWAYMKNE
                     MGDFRDFVMHEPRGHSDMFGAFLTSPEREDSHYGVIFMDSGDGVTMCGHGSIGTAHAL
                     VELGMVPRTEPYTEVVLDSPAGQIRLKVEVNDGRVGDITLANVPAFVFKRDVELAIPS
                     LGGKKIHMDVSFGGNFFAIVHAEEMGLTLERNEVPRLLSLGLEILREANRAVKVQHPT
                     ERHLDFIGLTEFALERPGKPTKNCVVFGESNVDRSPCGTGTCAKMALLAAKGLLKPGE
                     VFDHESVAGSVFHGHYEPGPKVGGFETILPFIRGRASITGFNFLLQQKNDEMGSGFLL
                     "
     gene            1904..3049
                     /locus_tag="PLM30_03685"
     CDS             1904..3049
                     /locus_tag="PLM30_03685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR54306.1"
                     /translation="MGRSTADVAIIGGGVHGCSIAWHLAKRGVKAVLFERDYISSGSS
                     GRSAAGLRYQFGTEVNCRLARYNMEKIPQLQEELGFKADLEYDPAGYLWIAYSETQLE
                     QLKKNVALQNSLGVGSVILDPDGIKEVAPGLDVRGMLGASFNEKDGHVNPHAMNFAYA
                     DAARGLGADIRTGTSVTGLLTDGHKVRGVRTARGDWETGAIICAVNAWSREVCGWCGL
                     DVPVTPERHQILVTEPVERMKHPMTLCLDDGCYWKQCPNGTFMFGWDNPEEKKDTSFE
                     SSWQFPEEVSKRVLAKMPCLAGVRVVRQWTGPYAVTPDEQAIMGKTPVENFFFVGGWS
                     GHGLQFAPSSGRIMSEIVLGEEPFLDVSCFRFSRFEEGCLYPEPACI"
     gene            complement(3093..3986)
                     /locus_tag="PLM30_03690"
     CDS             complement(3093..3986)
                     /locus_tag="PLM30_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR54307.1"
                     /translation="MEGKTLAIKVDVDTLKGYLEGVPRLLDILWRRDLRASFFFSFGP
                     DNSGRAVRRVFRKGFIGKMLRTDAPGTYGLKTMLYGTLLPAPLIVPRDPGIFRRVLSE
                     GHEGGIHAWDHVTWQDKLDRMPEPRMREHFDRAFEMFRDLSGSEPRCCAAPAWKAAAS
                     SLAMQDGYGFDYCSDTRGVSPFIPRMGGRVFVTPQVPTTLPTMDELLGTAGIGEGNLN
                     SHLLGLMGTGLNVHTVHAEMEGGSRSGLFEELLDRCLELGVDITSTGEALGRLEKGAL
                     PVMDVVQKSIPGRAGKVAVQA"
     gene            4193..>4602
                     /locus_tag="PLM30_03695"
     CDS             4193..>4602
                     /locus_tag="PLM30_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54308.1"
                     /translation="MRLSPFLSFFGIALGVYGAANLFIWFHLARVAPYLGVFKTPVVI
                     AFWLLAAAFPAGRVGGAFCRCALTDQLTILGSWYLGFMVYVLLFLILVDVLRLADHWV
                     RIVPRSLAGGRGALAAFWGVLLLSVAVVAAGRWNA"
BASE COUNT          810 a         1483 c         1474 g          835 t
ORIGIN      
        1 ggctcccacc tcctctggta gaagtataaa cggttacagt attccggcca cccccacggc
       61 ggtccacacc agcaaaaggg aaaagcccag gttgatgatc ctgtgatgga gggggttctg
      121 caggaaacga tggatgccga gaccgaagaa ggcccaggcc atgatcgagg tgaagcctat
      181 agcggcgacc agggcggccg acaggaaaac cggggtcggt cgggcgagga tgggccccag
      241 gaaggcggta tagagggtga tcgagaagag gatggactta gggttggcga actggagcac
      301 gaaccccttg gcgaagcccc tcatgggctc cccgcccccc atgcccccct ggccctcgct
      361 ccgggcgatg ccgtaggcca gccagaggat gtaggcggca cctacccacc taaggtaccc
      421 ctcgagcgcg gggagggcgc ttatgagcgc ccccgagaaa gcccccgcga gcaggaggac
      481 gacgaaaaaa cccatgccta tgcccaggag gtaaccccag gtccgcctca taccccaccg
      541 ggccgcggcg gcggcgcagc ttatgttgtt cggccccggc gtgaaggtgg tgacaaacac
      601 gtaggggatc agggcgacga agtttatctc ctccagcatt tcctctcccg gcctttccct
      661 aatttattat ccacgggctt gcccgcccgg cgcgttcctt acagcaggaa acccgagccc
      721 atctcgtcgt tcttctgctg aagcaggaag ttgaaaccgg tgatactcgc ccgtcccctg
      781 atgaagggga ggatcgtttc gaaaccgccg accttggggc ccggctcgta atggccgtgg
      841 aagacgctcc cggcgacgct ctcgtgatcg aagacctccc ccggtttcag aaggcccttc
      901 gcggccagca gcgccatctt ggcgcaggtc cccgtcccgc agggggaccg gtctacattg
      961 ctctccccga agacgacgca gttcttcgtg ggcttgccgg gccgctccag ggcgaactcg
     1021 gtcaggccga tgaagtccag gtgcctctcg gtggggtgct gaaccttcac ggcgcggttg
     1081 gcctcgcgga gtatctcgag ccccagtgag agcagccggg gcacctcgtt cctctccagg
     1141 gtgaggccca tctcctcggc gtggacgatg gcgaagaagt tgccgccgaa ggagacgtcc
     1201 atgtggatct tcttcccgcc cagggagggg atggccagtt ccacgtcccg cttgaagacg
     1261 aaggccggga cgttggccag ggttatatcg cccactctcc cgtcattgac ttccaccttg
     1321 agtcggatct gcccagccgg ggagtcgagg accacctcgg tgtagggttc cgtcctgggc
     1381 accatgccca gttcgaccag ggcgtgggca gtgccgatgg agccgtgccc gcacatggtg
     1441 accccgtcgc cggaatccat gaatataacg ccgtagtgag agtcttccct ctcgggggag
     1501 gtcaggaagg caccgaacat gtcgctgtga ccccggggct cgtgcatgac gaagtcgcgg
     1561 aaatctccca tctcattctt catgtaggcc catctctcgc tcatggtctt gccggggatg
     1621 tagggtcctc cgccgatgat tatcctcgta ggttcgcccc cggtatgggt gtctactact
     1681 gctgtggcac gggtcagttc catgtgatcc atctcctttc agtcgtcctg gggaatgcag
     1741 gtcccgatta taggcggtat tgtaccagca acggcgaggg gcggcatttg actggaggac
     1801 gggtagaaaa tagaatctgt tcaaaggtcc agagcattat atccggggac cggcggcggg
     1861 gcaacggccg gtcttcactc tctcgcgaaa ggggtcgggc ggggtgggtc gttcaacagc
     1921 ggacgtggcg ataatcggtg gcggggtaca cggctgttcc attgcctggc acctggcgaa
     1981 aagaggggtg aaggcggttc tcttcgagag ggattatata tcctccggat cgagcggccg
     2041 cagcgcggcg ggcctcagat accagttcgg caccgaggtc aactgcaggc tggcccggta
     2101 caacatggag aagatccccc aactccaaga ggaactgggc ttcaaggccg acctggaata
     2161 cgaccccgcc ggctaccttt ggatagccta cagcgagacc cagctggaac agctgaaaaa
     2221 gaacgtggca ctccagaact ccctcggcgt cggttccgtc atcctcgacc ccgacgggat
     2281 aaaggaggtg gccccggggc tggacgtgag gggcatgctc ggcgccagct tcaacgagaa
     2341 ggacggccac gtcaaccccc atgccatgaa cttcgcctac gccgacgccg cacggggcct
     2401 cggggcggac atcaggaccg ggacgtccgt cacgggcctt ctcaccgacg gccataaggt
     2461 ccgaggcgtc aggaccgcga ggggggactg ggagacgggg gccatcatct gcgccgtcaa
     2521 cgcctggtcc agggaggtct gcggttggtg cggcctggac gtgcccgtga cgccggagcg
     2581 ccaccagatc ctcgtcaccg agccggtgga gaggatgaag caccccatga ccctttgcct
     2641 cgacgacgga tgctactgga agcagtgccc caacggcacc ttcatgttcg gatgggataa
     2701 ccccgaggag aaaaaggaca ccagtttcga gtcatcctgg cagttccccg aggaggtctc
     2761 gaaaagggtg ctcgccaaga tgccctgtct ggccggcgtc cgcgtcgtcc gccagtggac
     2821 gggcccttac gccgtgaccc ccgacgagca ggccattatg ggcaagacac cggtagagaa
     2881 cttcttcttc gtcggcgggt ggagcggcca cggcctgcag ttcgcgccct cctcgggaag
     2941 gatcatgtcc gagatagtcc tgggcgagga acccttcctc gacgtcagct gcttccgctt
     3001 cagccggttc gaggagggct gtctctaccc ggaaccggcc tgtatctgat cggcatcgtg
     3061 gccgggatgg gccgtccccg gcctcggcgc ggtcacgcct ggacggccac cttccccgcc
     3121 cttccgggta tgcttttttg gaccacgtcc atcaccggga gcgctccctt ttcgaggcgc
     3181 cccaaggcct cccctgtgct ggtgatatcg accccgagtt cgaggcaccg gtcgagcagc
     3241 tcctcgaaca ggccggacct ggagccccct tccatctcgg cgtggaccgt gtggacattc
     3301 agccccgtcc ccatgaggcc gagcaggtgg gagttcaggt tgccctcgcc gatgccggcc
     3361 gtccccagca gttcgtccat cgtgggcagc gtcgtgggga cctggggagt tacgaagacc
     3421 cgtcccccca tccgggggat gaagggggaa acgccccggg tgtcgctgca gtagtcgaag
     3481 ccgtacccgt cctgcatggc caggcttgaa gccgccgcct tccaggccgg ggcggcgcag
     3541 cagcggggct cactgccgga aagatcccgg aacatctcga aggccctgtc gaagtgttcc
     3601 ctcatcctgg gctcaggcat acggtcgagc ttatcctgcc acgtcacgtg atcccaggcg
     3661 tggataccgc cctcgtgacc ctccgacagg acgcggcgga agataccagg gtcccgcgga
     3721 acgatcagcg gcgccggcag cagcgtgccg tagagcatgg tcttcagacc gtaggtgccg
     3781 ggtgcgtcgg tgcggagcat cttgccgatg aaacccttgc ggaaaacccg ccggacggcc
     3841 ctgcccgagt tatcgggccc gaaggagaaa aagaaggaag cccggagatc ccgcctccag
     3901 aggatgtcca gcagcctcgg gacaccctcc aggtagccct tgagggtatc cacgtcgacc
     3961 tttatcgcga gggtctttcc ctccaccgcc actccccctt ttaagccggg gtccgacccc
     4021 gggcgttccc cgcttccatg gtgacacggc cggaccccgg ggccgctccc acccttgaat
     4081 cctagatggt aatccccttg ccgtaaacct cgaaggggtc cgcgggacgg tttacgaacc
     4141 gccccctacg gcttatgatc aggtaactga aggtccacct acaggaggtt ccatgcggct
     4201 ttccccgttc ctttcctttt tcggcatcgc cctcggcgtc tacggggcgg caaacctctt
     4261 catatggttt cacctcgcca gagtcgcccc ctacctcggg gtcttcaaga cgcccgtcgt
     4321 catcgctttc tggctcctcg cggcggcctt cccggcgggg agggtcggcg gcgccttctg
     4381 ccggtgcgcc ctgacggacc agctcactat cctggggtcg tggtacctgg gtttcatggt
     4441 ctacgtcctt ctcttcctga tccttgtcga tgtcctccgg ctggccgacc actgggtcag
     4501 gatcgtaccg cggtccctcg ccgggggccg gggggcgctg gccgccttct ggggggtcct
     4561 cctcctgtcg gtggccgtcg tcgcggcggg gcggtggaac gc
//
LOCUS       DAOSRG010000071         4577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_17137_length_4577_cov_8.344759, whole genome shotgun sequence.
ACCESSION   DAOSRG010000071 DAOSRG010000000
VERSION     DAOSRG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17137_length_4577_cov_8.344759"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLM30_03700"
     CDS             <1..879
                     /locus_tag="PLM30_03700"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOR54309.1"
                     /translation="VVVGRNQNPWAECRLDLLESEGGKLARRSTGGGAVYHDLGNLNY
                     SFILPRKRYSMERQLAVILGTLRGLGLDAEFSGRNDLVIGGRKFSGNAYQLTRDKGLH
                     HGTLLFSADMAMLSRYLNVDPEKLRTKGVASVASRVMNLRDASPGLTLADLQDALEEE
                     FLARYPAAERLREQGAPGGEEFQRLARRYRSREWRYGRSPGCEVSLRRRFPWGGLEVR
                     FDVRDGRVSSPVVFSDAMDGEMILALGGMLEGVPFRWKEMAEAVRRGFPGRREMAETA
                     AWLEEAPVGERKEACS"
     gene            876..1988
                     /gene="gcvT"
                     /locus_tag="PLM30_03705"
     CDS             876..1988
                     /gene="gcvT"
                     /locus_tag="PLM30_03705"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOR54310.1"
                     /translation="MKRTDMYERHVAAGGRMVDYSGWELPVQYDGTGIKAEHLNVREK
                     AGLFDVSHMGEIMVEGPDAEKWLSTLVTNDIFMMRDGQVQYNIMCNESGGVVDDLLVY
                     RYGPAKFLLVVNAANVDKDREWLTKHLPPRGAAIDDISMKTSETALQGPAAEAILARV
                     ADFDPSTLKFFTFTHGANLGGIPAIVSRTGYTGEDGFEIYVPWERGAELWDLLIEAGR
                     DFGLIPVGLGARDSLRFEAGLPLYGHEYAEDLGPLEAGFGFFVKVDKPGGFIGQPVLK
                     RQKEEGLQRKIVGLRLEDKGVPRSGMDVADPSGNIVGRVTSGGYCPSLDANMALCLVN
                     MPAPEEGGRLSVIVRDKPKKGVVVKRPFYARKRGNR"
     gene            2026..2424
                     /gene="gcvH"
                     /locus_tag="PLM30_03710"
     CDS             2026..2424
                     /gene="gcvH"
                     /locus_tag="PLM30_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOR54311.1"
                     /translation="MAKIPQDLRYTKDHEWIRMEDGVGVVGITDYAQHAMGDVVYVEF
                     PEKGRKVHAGEDFVVVESVKGANDVFSPVSGTVLEVNDALEESPELLNQDAYANWLVR
                     LEVADPGELNSLLDHAAYAALLEELEAENG"
     gene            2437..3786
                     /gene="gcvPA"
                     /locus_tag="PLM30_03715"
     CDS             2437..3786
                     /gene="gcvPA"
                     /locus_tag="PLM30_03715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOR54312.1"
                     /translation="MSRYTPATAEDRRRMLEYIGVGSIDDLYSGIPAGVRQKDLPDIP
                     KALSEPEVIRDLRELAGANRNIDDLPCFLGAGIYDHFIPAAVDSIISRGEFTTCYTPY
                     QPEVSQGTLQAIFQYQTMVCELTGMEVSNASMYDGASATAEAAVLAASATKRKKILWG
                     RNVNPQTREVLRTYCWSRGLEHGEVPAVGGTIDREALDGALSAGDAAALVVQSPSFFG
                     TVEDIKALAEKVHGAGALLIVVTDLLALGTLEAPGALGADIVAGDGQTVGNPASFGGP
                     AFGFIAVNKPLMRKMPGRICGQTVDSRGRRAFVLTLQAREQHIRREKATSNICSNQNL
                     CIVAASVYLSLLGPQGLAEVGGQIMAKTEYALGRLEKSGKFRRAFPGVPYFREAVLLS
                     ERPVKELNRRLLDAGIIGGYPLEPLYPELENGWLLAVTENRTKQEIDRLVAVAGGDA"
     gene            3786..>4577
                     /locus_tag="PLM30_03720"
     CDS             3786..>4577
                     /locus_tag="PLM30_03720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164423.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOR54313.1"
                     /translation="MTRRMETVFEASRPGRKGYALPELDVPAVDCRAALGKACRGKPP
                     RLPEMAEVDVVRHFTRLSKLNYSVDEGFYPLGSCTMKYNPKVNEVAARMPGFARLHPL
                     QPEETAQGAMKLLFDLGAMLAEITGMDAVTLQPAAGAHGEQTGLMLIKAWHESRGDAK
                     RTKVIVPDSAHGTNPASAAITGFETVEVASDDEGMVDLGALEAILDDTVAALMLTNPN
                     TLGIFEKHILELTEAVHRAGGLVYYDGANLNAIMGKARPGDMGFDV"
BASE COUNT          853 a         1493 c         1551 g          680 t
ORIGIN      
        1 gtggtggtgg ggcgcaacca gaacccctgg gccgaatgcc gcctggacct cctcgagtcg
       61 gaggggggca aactcgcgcg gcgttcgacc ggcggggggg ccgtctacca cgatctcggc
      121 aacctcaact actcctttat ccttcccagg aagcgctaca gcatggaacg gcagctggcc
      181 gtcatcctcg gcaccctccg ggggctcggc ctcgacgccg agttctccgg caggaacgac
      241 ctggtcatcg gcggcaggaa gttctcgggc aacgcctacc aactgaccag ggacaagggg
      301 ctccaccacg gcaccctcct cttctccgcc gacatggcca tgctctcccg gtacctgaac
      361 gtggaccccg aaaaactaag gaccaagggg gtggcctcgg tggcctcccg ggtgatgaac
      421 ctccgggacg cttcgccggg cctgaccctg gcggacctcc aggacgccct cgaagaggag
      481 ttcctcgccc gctatcccgc cgccgaacgg ctcagggaac agggagcgcc cgggggggag
      541 gagttccagc gcctcgccag gcgctaccgg agccgggagt ggcgttacgg ccgctcccca
      601 ggctgcgagg tctcgctccg gcggcgcttc ccctgggggg gtctggaggt ccgcttcgac
      661 gtgagggacg gccgcgtctc ctccccggtg gtcttttccg acgccatgga cggcgagatg
      721 atcctcgccc tgggcgggat gctggaaggc gtgcccttcc ggtggaagga gatggccgaa
      781 gcggtcagga ggggtttccc cggccggcgg gagatggccg aaacggccgc ctggctcgag
      841 gaagcccccg taggtgaaag gaaggaggcc tgttcatgaa gcgcaccgat atgtacgaaa
      901 ggcacgtcgc ggcaggcggg cggatggtcg attacagcgg atgggaactg ccggtccagt
      961 acgacggcac cggcataaag gccgagcatc tcaacgtcag ggaaaaggcg gggctcttcg
     1021 acgtctccca catgggggag atcatggtgg aaggccccga cgcggagaaa tggctctcca
     1081 ccctcgtgac gaacgacatc ttcatgatga gggacggcca ggtgcagtac aacatcatgt
     1141 gcaacgagtc ggggggcgtg gtggacgacc tgctcgtcta ccggtacggc ccggcgaagt
     1201 tcctcctcgt ggtgaacgcg gccaacgtgg acaaggaccg ggaatggctc acgaagcacc
     1261 tgcccccgag gggcgctgcg atcgacgaca tctccatgaa gacctccgag acggcgctcc
     1321 aggggccggc ggcggaagcc atcctggcca gggtggccga cttcgacccg tcgaccctca
     1381 agttcttcac cttcacccac ggggccaacc tggggggcat ccccgccatc gtcagcagga
     1441 ccggctacac cggcgaggac ggcttcgaga tctacgtccc ctgggagagg ggtgccgaac
     1501 tctgggacct gctcatcgag gcggggagag acttcggcct gatccccgtc gggctcggtg
     1561 cccgggacag cctccgcttc gaggcggggc tcccgctcta cggtcacgag tatgccgagg
     1621 acctgggccc cctcgaggcc ggtttcggtt tcttcgtcaa ggtggacaag cccggcggtt
     1681 tcatcggaca gccggtgctg aagcgccaga aggaggaggg cctccagcgg aagatcgtcg
     1741 gcctgcgcct tgaggacaag ggggtgcccc gatccgggat ggacgtggcc gacccctcgg
     1801 gaaacatcgt gggcagggtg acctccggcg gttactgccc ctcgctggac gccaacatgg
     1861 ccctatgcct cgtgaatatg cctgcgccgg aggagggcgg ccgcctgagc gtcatcgtca
     1921 gggacaagcc gaagaagggc gtcgtcgtga agaggccctt ctacgccagg aaaagaggca
     1981 accggtaagg acgctttctc aaaagacgaa gggaggatga aaggaatggc caagataccc
     2041 caggatctcc gttacaccaa ggatcacgag tggataagga tggaagacgg cgtcggcgtc
     2101 gtggggatca cggactacgc ccagcacgcc atgggtgacg tcgtctacgt ggagttcccc
     2161 gaaaagggca ggaaggtcca tgccggggag gactttgtcg tggtcgagtc ggtgaagggc
     2221 gccaacgacg tgttcagccc cgtctccggg accgtcctcg aggtgaacga cgccctcgag
     2281 gagtcgccgg aactactgaa ccaggacgcc tacgccaact ggctcgtccg cctcgaggtc
     2341 gccgaccccg gcgagctgaa ctcccttctg gatcacgcag cctacgccgc gctcctcgag
     2401 gaactcgaag cggaaaacgg ataggaggtg tctttcatga gccggtacac accagccacg
     2461 gcggaagacc ggcgccggat gctcgaatac atcggcgtcg ggtccatcga tgacctctac
     2521 tcgggcatcc cggccggtgt aaggcagaaa gacctcccgg atatcccgaa ggccctttcg
     2581 gagcccgagg tcatccggga cctccgggaa ctggccggcg ccaacaggaa catcgacgac
     2641 ctgccctgct tcctcggcgc ggggatctat gaccacttca tccccgccgc ggtggacagc
     2701 atcatctccc ggggggagtt caccacttgc tacacgccct accagccgga ggtgtcccag
     2761 ggcaccttgc aggccatatt ccagtaccag accatggtct gcgaactgac cggcatggag
     2821 gtctccaacg cctccatgta cgacggcgct tccgcgacgg ccgaggcggc cgtcctggcg
     2881 gcgtcggcga ccaagagaaa gaagatcctc tgggggagga acgtaaaccc ccagaccagg
     2941 gaggtcctcc gcacctactg ctggagcagg ggcctcgagc acggtgaagt gcccgccgtt
     3001 ggcggcacga tcgaccggga agccctggac ggggcccttt cggcgggcga cgcggcggcc
     3061 ctggtggtcc agtcgcccag cttcttcggc accgttgagg acatcaaggc cctcgccgaa
     3121 aaggtccatg gtgcgggggc gctcctcatc gtcgtcacgg acctgctggc cctcgggacc
     3181 ctcgaggcac cgggcgcgct cggcgccgac attgtcgcgg gcgacgggca gaccgtcggc
     3241 aaccccgcca gtttcggcgg gcccgccttc gggttcatcg ccgtgaacaa gcccctcatg
     3301 cgcaaaatgc ccggccgcat ctgcggccag accgtcgaca gccgggggag gcgggccttc
     3361 gtcctgaccc tccaggccag ggagcagcac atccggaggg agaaggccac gtcgaacatc
     3421 tgctccaacc agaacctctg catcgtggcg gcctcggtct acctgagcct cctggggccc
     3481 caggggctcg ccgaggtggg cgggcagatc atggcgaaga cggagtacgc cctggggcgg
     3541 ctcgaaaaga gcggcaagtt caggagggcc ttccccgggg tgccctactt cagggaggcg
     3601 gtgctcctca gcgaaaggcc cgtcaaggaa ctcaacaggc gcctgctcga cgccggcatc
     3661 atcggcgggt acccactaga gccgctctac cccgaactgg agaacggctg gctcctggcg
     3721 gtgacggaga acaggacgaa acaggagatc gaccgccttg tggcggtcgc gggaggtgac
     3781 gcctgatgac ccgcaggatg gaaaccgtct ttgaagcgag ccggccgggg aggaagggct
     3841 acgccctgcc cgaactggac gtcccggccg tcgactgccg cgcggccctc ggaaaggcct
     3901 gccgcgggaa gccgcccagg ctgcccgaga tggcggaagt ggacgtggtg cgccatttca
     3961 ccaggctgtc aaaactgaac tactccgtgg acgagggttt ctaccccctc ggttcctgca
     4021 ccatgaagta caaccccaag gtcaacgagg tggcggcgag gatgccgggt ttcgcgcgcc
     4081 tccaccccct ccagccagag gagacggccc agggggcgat gaaactgctc ttcgacctcg
     4141 gcgccatgct ggccgagatc accggcatgg acgccgtcac ccttcagccc gccgcgggag
     4201 cccacggcga acagaccggg ctgatgctca taaaggcgtg gcacgagagc cggggcgacg
     4261 caaagaggac caaggttatc gtccccgatt cggcccacgg gaccaacccg gcatcggccg
     4321 ccatcacggg tttcgagacc gtggaggtgg cctccgatga tgagggcatg gtggacctcg
     4381 gcgccctcga ggccatcctg gacgataccg tggcggccct catgctgacg aaccccaaca
     4441 cgctgggcat cttcgagaag catatcctcg agctcaccga ggccgtccac cgggccgggg
     4501 gcctggtcta ctacgacggg gcgaacctga acgccatcat gggcaaggcc cgccccggcg
     4561 atatgggttt cgacgtc
//
LOCUS       DAOSRG010000072         4497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_17508_length_4497_cov_9.627870, whole genome shotgun sequence.
ACCESSION   DAOSRG010000072 DAOSRG010000000
VERSION     DAOSRG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_4497_cov_9.627870"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /gene="pheT"
                     /locus_tag="PLM30_03725"
     CDS             complement(<1..1769)
                     /gene="pheT"
                     /locus_tag="PLM30_03725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOR54314.1"
                     /translation="MFASLNWLKELVDIPVDTPMLAQRLTDTGVEVEGLEVPVALFRG
                     AVTARVASIAIHPGRPDLFVLTVDAGKRKGVCVTAARNLEEGDVVPWGPPGCVLADGT
                     EISCRDFQGVTSEGMVLSAEEIGLPGIADEYGILKMGEGFTPGEDARAALGLDDTVLE
                     LSITPDRGDLLSMVGLAREVSALFPEARYKGITPAIRPRASRLDGFEGITLTDSGCPF
                     YALGALEGVNIKPSPMRARVRLLLSGMRPISNVVDATNLVMLLVGQPLHAFDDARLPG
                     RDITVRLAREGEMILTLDGKTRVLDGEDLLITSGGKPVAVAGVMGGEESEIGGDTTRV
                     LLESAHFDSIKVSRASRKLGIPSEAAYRFSRFVDPCKAETALAVALEMMASWGAGVPA
                     GWIAAGEAAQKERKVALTSKVLGKIIGTGDLDMATAILERLGIYTGDRDPSVRYYSIP
                     SFRPDISIEEDLIEEVARIRGYDKVAAELPPVMHATGDITDRMRAERAVRSTAMARGY
                     SEVVTYCFVSPQSIAALHFPGPDPRSSPVALSNPLSVDMSVLRTTLVPGLVQAVLKNI
                     SSGWRRAIRIFETGMVFFRRGREV"
     gene            complement(1769..2782)
                     /gene="pheS"
                     /locus_tag="PLM30_03730"
     CDS             complement(1769..2782)
                     /gene="pheS"
                     /locus_tag="PLM30_03730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOR54315.1"
                     /translation="MEALFRSALSTADSVEALQGVRVRFLGKKGEVTSLLKRLGSLPA
                     DDRPEAGRVLNELRDSMEGSLAEKTRQYAGAQVTAREKEEWIDVTLPGRGRPWGAFHP
                     VAQVTQEAIDIFVSMGFSVAYGPEVEDDFHNFEGLNIPPHHPARDMQDTFYLENGLLL
                     RTHTSPVEVRSMRKWGAPLRIIVPGKVFRRDSDQTHTPMFHQLEGLLVDENISLAHLK
                     GCLEAFVNRIFGRPLKARYRGSYFPFTEPSIEMDVECVACSGRDPGCRICKGTGWLEI
                     SGLGMTHPNVIRAGGIDPSRYNGFAWGMGLDRIAMLKYGLNDLRVFFEGDLAFLNSKG
                     ERR"
     gene            complement(3067..3426)
                     /gene="rplT"
                     /locus_tag="PLM30_03735"
     CDS             complement(3067..3426)
                     /gene="rplT"
                     /locus_tag="PLM30_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOR54316.1"
                     /translation="MPRVKGGSASDQKRKKLFDVTKGYFGRKKNVFRRAQEAYLHSMS
                     NAYKDRKARKRDFRKLWITRISAGARMNDMTYSTFMNGLRCANIEINRKVLADLAVQD
                     MPAFARLVDRAREALVK"
     gene            complement(3477..3674)
                     /gene="rpmI"
                     /locus_tag="PLM30_03740"
     CDS             complement(3477..3674)
                     /gene="rpmI"
                     /locus_tag="PLM30_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOR54317.1"
                     /translation="MPKMKTHSGAKKRFKRTGTGKFVFQKSGRRHLLRTKGPARLRRL
                     RRKGVIASSLEERMKTLLPYA"
     gene            complement(3731..4333)
                     /gene="infC"
                     /locus_tag="PLM30_03745"
     CDS             complement(3731..4333)
                     /gene="infC"
                     /locus_tag="PLM30_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOR54318.1"
                     /translation="MAKNIEEPRTNDEIRAKEVLLIDDQGVKVGVVPLAQALALAEER
                     ELDLVEVSAQADPPVCRILDYGKYRFQQQKRDKDARKKSKGQVVKEMKMRPKIDLHDY
                     NFKVRAIKDFLLSGYRVKVAVFFRGREMAFLDRGREVLDKVVRDCEGIGKVDMPPRME
                     GRYMRLMMMPVPQSVKKPKAVVTSAAAAGETEKTKISVEE"
BASE COUNT          817 a         1467 c         1280 g          933 t
ORIGIN      
        1 acctcccggc ccctcctgaa aaagaccatg cctgtctcga agatccttat ggccctcctc
       61 cacccggaac tgatattctt caaaacggcc tggaccagcc ctgggacaag ggtagtcctc
      121 aagactgaca tgtcgacgct cagggggttg gagagggcca ccggggacga cctcgggtcc
      181 ggccccggaa aatggagcgc cgcgatggac tgcggggaga caaagcagta ggtcaccacc
      241 tcggaataac cccttgccat ggcggtcgag cggacggccc gttcggccct catcctgtcg
      301 gtgatgtcgc cggtggcatg catgaccggg ggcagctccg ccgccacctt gtcataaccc
      361 cttatgcggg ccacttcctc gatgaggtcc tcctcgatgg agatgtccgg cctgaaggaa
      421 gggatgctgt aatacctgac agaagggtca cggtcgccgg tgtagatccc aagcctctcg
      481 agaatggccg tcgccatgtc aaggtccccc gtcccgatga tcttgccgag gaccttcgag
      541 gtaagggcga ccttccgttc cttttgggcg gcttctccgg cggcgatcca cccggcgggg
      601 acgcccgccc cccaggaggc catcatttcc agggccaccg cgagggccgt ctctgccttg
      661 caggggtcca cgaagcggga gaaccggtag gccgcttcgc tggggatacc cagcttccgg
      721 gatgccctgc tgacctttat gctgtcgaag tgggccgatt ccagtaatac ccgggtggta
      781 tccccgccga tctcgctttc ctccccgccc atcacgccgg cgacggcgac gggcttcccc
      841 ccactggtga taaggaggtc ctcgccgtcc aggaccctgg ttttgccgtc gagggtcagg
      901 atcatctcgc cctcccgggc cagcctcacg gtgatatccc tgcccgggag gcgggcgtcg
      961 tcaaaggcat ggaggggttg tcccaccagg agcatgacca ggttggtcgc gtcaactaca
     1021 ttggagatag gcctcatgcc ggagagaagg agcctgaccc tggctctcat cggtgaaggt
     1081 ttgatgttga ccccctccag agcgccgagg gcgtagaaag ggcagccact gtcagtgagg
     1141 gtgatcccct cgaaaccgtc gagccgcgaa gccctgggcc ggatcgccgg ggttattccc
     1201 ttgtacctcg cctcggggaa gagggccgag acttccctgg ccaggccgac catgctcagg
     1261 aggtcgcccc tgtcgggcgt tatggaaagt tccaggaccg tatcgtcgag accgagggcc
     1321 gccctggcgt cctccccggg ggtaaagccc tcccccatct ttagtatccc gtactcatca
     1381 gcgatcccgg ggaggccgat ctcctcggcc gacagcacca tgccctcgct ggtcacaccc
     1441 tggaaatccc tgcaggagat ctccgttccg tcggccaaga cgcaccctgg ggggccccag
     1501 gggaccacgt cgccctcctc taggttcctg gccgctgtca cgcacacccc tttcctcttt
     1561 ccggcatcga cggtgaggac aaaaaggtcc ggcctcccgg ggtgaatagc gatggaagcg
     1621 accctggctg tcacggcgcc gcggaacagg gccaccggga cttcaagtcc ctccacctcg
     1681 accccggtgt cggtgagcct ctgggccagc ataggggtat ctacggggat atccaccaat
     1741 tccttgagcc agttgagtga tgcgaacatt atcggcgctc tcccttcgag ttgaggaaag
     1801 ccaggtcgcc ctcgaagaag accctcaggt cgttcagccc gtacttgagc atggcgatac
     1861 ggtcgagtcc catcccccag gcgaagccgt tgtagcggga cgggtcgatc cccccggccc
     1921 ggatgacgtt ggggtgggtc atccccagcc ccgagatctc cagccagccg gtccccttgc
     1981 atatccggca acccgggtcc ctgcccgaac aggctacgca ctctacgtcc atctctatgg
     2041 agggttccgt gaaggggaag tagctgcccc tgtaccgggc cttcaacggg cggccgaata
     2101 tccggttgac aaaggcctcc aggcaaccct tgaggtgggc cagggagatg ttctcgtcca
     2161 ccaacagccc ctcaagctgg tggaacatgg gggtgtgggt ctgatcgcta tccctccgga
     2221 agaccttccc ggggacgatt atcctcaggg gggcgcccca tttcctcatg gacctcacct
     2281 ctaccgggga ggtatgggtc ctcaggagca gcccgttctc aaggtaaaag gtgtcctgca
     2341 tatccctggc cgggtggtgg ggcggtatat tcagaccttc gaagttatgg aagtcatcct
     2401 ccacctcggg gccataggcc acggaaaaac ccatggagac gaatatgtcg atcgcttcct
     2461 gggtgacctg ggccacgggg tggaaggcac cccagggacg gcctctgccg gggagggtca
     2521 catcgatcca ctcctccttt tcgcgggcgg tcacctgggc gccggcgtac tgcctcgtct
     2581 tttccgccag ggacccctcc atggaatccc tcaattcgtt gagcaccctg ccggcctcag
     2641 ggcgatcatc ggcagggagc gaacccaacc gtttgagcag ggaggtgacc tcccccttct
     2701 tgccgaggaa acggaccctc actccctgca gcgcctccac ggaatccgcc gttgaaaggg
     2761 ccgatcggaa caaggcctcg atccggctta catcatcgtg aatatcactc aagcgcttcc
     2821 cctccctggc gggttcaagc acccgtgagt tctcccgtcc tgaccggggc ggacctgcaa
     2881 aaggccgccg atccaagccc gggagagaag gaacgacccc gaccagtata atacctttgc
     2941 ggggctgaat cggcccttat tctacccttc accgccggtt atacggcaag ggtccccgcc
     3001 aaagcaatgg acaggaaccc tgggctgtac gaccgatacg gtccggtacg tcaggtgggg
     3061 aggcgtttac ttcaccagcg cctccctcgc cctgtcgacg agcctggcga aggccggcat
     3121 gtcctggacc gccaggtccg cgagtacctt gcggttgatc tcgatgttag cgcatcggag
     3181 accgttcatg aacgtgctgt aggtcatatc gttcatccgc gccccggcgc ttatcctggt
     3241 tatccagagc ttgcggaagt ctctcttcct ggccttcctg tccttgtacg cgttggacat
     3301 ggaatgaagg tacgcttcct gcgccctccg gaaaacgttc ttttttcttc cgaagtagcc
     3361 ctttgtaaca tcaaaaagct tctttctttt ctgatcactg gcgcttccgc ccttgaccct
     3421 gggcatgacc gctcacctct ttcggttggt tgataccaga tcgttcgttc cgacgcctag
     3481 gcgtagggca gaagagtttt catccgttcc tcgagggaag aggctataac acccttccgg
     3541 cgaaggcgac gtaggcgcgc ggggcccttc gtcctgagaa ggtgcctgcg gccgctcttt
     3601 tgaaaaacaa atttccctgt ccctgtcctc ttgaacctct tcttggctcc cgagtgggtt
     3661 ttcatcttag gcatgatcca attcctcctt cattctgaaa cgacgggaat aaaaaagacc
     3721 cgcccttggg tcactcttcg acggaaatct ttgtcttttc cgtttccccg gcggctgcgg
     3781 cgcttgtcac caccgccttt ggcttcttca ccgactgcgg gacgggcatc atcatcaggc
     3841 gcatatacct tccctccatt cggggaggca tatcgacctt cccgatgccc tcgcaatccc
     3901 ttacgacctt gtccagaacc tcccggcccc gatcaaggaa cgccatctcc cttcccctga
     3961 aaaagacggc caccttgacc ctgtaacccg agaggagaaa atccttgatg gcacgcacct
     4021 tgaagttgta gtcgtgcagg tctatcttag gtctcatctt catttccttg acgacctgcc
     4081 ccttggactt tttcctcgcg tccttgtccc tcttctgctg ctggaaccgg tacttgccgt
     4141 aatcgagtat cctgcagacg gggggatcgg cctgggcgga aacctccacc aggtccagct
     4201 ccctctcttc ggcaagggcc agtgcctgtg ccagggggac caccccgacc ttgaccccct
     4261 ggtcgtcgat caggaggact tccttggccc ggatctcatc gttggtgcgt ggctcttcga
     4321 tgtttttagc tatagcatgt cacctccaca aaaaaaaata tgccggactc agaaaagtcc
     4381 ggcccctatg tcccatatgt cccaactggg cgaaatctct ttcgcttcgc tgatacagaa
     4441 cccttgaaca accccgttcg gggcgtcggc gggtgggagg ctcggctcct cttcatc
//
LOCUS       DAOSRG010000073         4455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_17702_length_4455_cov_9.669545, whole genome shotgun sequence.
ACCESSION   DAOSRG010000073 DAOSRG010000000
VERSION     DAOSRG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17702_length_4455_cov_9.669545"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..250
                     /locus_tag="PLM30_03750"
     tRNA            165..250
                     /locus_tag="PLM30_03750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Leu,seq:cag)
     gene            422..4036
                     /locus_tag="PLM30_03755"
     CDS             422..4036
                     /locus_tag="PLM30_03755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR54319.1"
                     /translation="MAEFVTVNSKRQRLTFGRTRDLVEIPDLVEIQRDSFRWFFQFNE
                     KGGLIEADERSSQGLQELFDEVFPIESYDGSFALEFVDYFVDPPQVTQEEAIQRDLSW
                     SRSVRATIRLVNRKTEEIKEEEVYLGDFPMMTERGTFIINGTERVVVSQLARSAGVYF
                     NSEESIPGQERYSAKVIPDRGAWLEFDMTPGEVLSVNIDNRKKLPVTVLLKAFGVAGN
                     DEILELFGAVEEQIDLIEDEVKGRLIAETITEESGKAVIRKNERITRDILERLLEMGR
                     TRAWVWKTDSAIEATMERDPTRNTDEAILELFRRLRPNEPARMENAREYVNGLFFDTR
                     RYNLGRVGRYKLNRRLGLDIPGSVRLLLVDGVVQIVKKLMGLRDGYERIDDIDHLGNR
                     RVRAVGELLQNQVRIGLLRMERIARERMTTIPDLTGATARELINVRPISAALREFYGS
                     GQLSQFMDQTNPLAELTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEG
                     PNIGLVTSLSIYSRINEYGFLQAPKRKVVDGKVTDEVVYLGADEEDEYYIGRANTPLN
                     SDRTFAEKEIYVRDRGDIAVVEPAKVDFLDISPKQIVSASTALIPFLEHDDANRALMG
                     SNMQRQAVPLINSRAPIIGTGIEARIARDSGSCVVARNPGTVIGVDSRTIKIQAGKAG
                     KVDQYNLVKFKRSNQGTIIHQKPIVFKGDKVKAGDIIADGQAVENTELALGNNVLVAF
                     VSWEGYNYEDAILLSDRLVKNDSFSSVHIEEYEVEARDTKLGPEEITRDIPNVGEDAL
                     KDLDETGIVRIGAEVVAGDILVGKVTPKGESDQSPEEKLLRAIFGEKAREVRDTSMRI
                     PHGEGGKVVAVKRLSRSENGDDLTPGVNEVVKVYVAKIRKISVGDKMAGRHGNKGVVS
                     RILPEEDMPYLPDGTPIDVVLNPIGVPSRMNLGQVLETILGFVASQNDWHVSTAVFEG
                     ATEQEILDLVEKSRQEGFKDMTPDGRVLLYDGRTGEPMRRPVTVGTMYMMKLIHLVDD
                     KIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGAAHVLQEILTVKSDDIR
                     GRLKTYERIVKGLNLTEHGVPESFRVLVKELQGLGLDVEIAYEDGSMGELLMDEDEEP
                     ALLPRVRTQEKREGPAGRPEGALDDLFHDEVPVEEDRGQLISVLGGESGDDPEEGAEE
                     "
     gene            4037..>4455
                     /locus_tag="PLM30_03760"
     CDS             4037..>4455
                     /locus_tag="PLM30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54320.1"
                     /translation="MNQRGISGVKIKLASPERIREMSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTRSFECACGKYKRSGPKFKGVVCDRCGVEIADNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGTATKDLEKVVYFAPTRRREP"
BASE COUNT         1001 a         1301 c         1435 g          718 t
ORIGIN      
        1 gcaaaggcaa aggcaaaggc aaaggcaaag gcaaaggcaa aagcaaaagc gaaaggcgtg
       61 aaccgtaaag gaacgaggtg ccttggcgcc gggccccttt accaaccacc tgttgcaaaa
      121 aggtcccccc gttgataaaa tcaacggcgt cggtaccagg acccggggaa gtggcggaac
      181 ggtagacgcg ctggcttcag gagccagtgt ccgtaagggt gtgggggttc aaatcccccc
      241 ttccccacca tagaatacgt ccaccccgtc ggcgggtggt gaaaaacggt gatcgcacca
      301 ggagaaaacc gaaagggttg tgctgtgcct attgacagag cacagccctt tcgctattct
      361 tttagtttgt cacgtctagt ttccccaaat cccctagaga cagggaggtt gtgtttctcc
      421 catggctgaa ttcgttaccg taaacagcaa gcggcagcgc ctcaccttcg gtcgcacccg
      481 cgaccttgtc gagatccccg atctggtgga gatccagcgg gattccttcc gctggttctt
      541 tcagttcaac gaaaagggcg gactcatcga ggcggatgag agatccagcc agggtctcca
      601 ggagctcttc gacgaggtgt tccccatcga gagctatgac gggtccttcg cccttgaatt
      661 cgtggactat ttcgtcgacc ccccccaggt cacccaggaa gaggcgatcc agagggacct
      721 ttcctggtcc aggtcggtca gggcgaccat ccgcctggtg aacaggaaga ccgaggagat
      781 caaggaagag gaggtctacc ttggggactt ccccatgatg accgaaaggg ggaccttcat
      841 catcaacggc accgagaggg tcgtggtcag ccagctggcc cgctcggcgg gcgtctactt
      901 caactccgag gaatccatcc ccgggcagga gcgttactcc gccaaggtca tccccgacag
      961 gggagcctgg ctcgagttcg acatgacgcc gggcgaggtc ctatcggtga acatcgacaa
     1021 caggaaaaaa ctgcccgtca cggtgttgct caaggccttc ggcgtagcag gcaatgatga
     1081 gatcctggag cttttcgggg ccgtcgagga acagatcgac ctcatcgagg acgaggtcaa
     1141 gggaaggctc atcgccgaaa ccataaccga ggagtccggg aaggccgtca tcaggaagaa
     1201 cgagaggatc acccgggaca tccttgagag attgctggag atgggcagga cccgggcctg
     1261 ggtctggaag accgactccg ccatcgaggc taccatggag cgcgacccga caaggaacac
     1321 cgatgaggcc atcctcgagc tgtttcgcag gcttcggccc aacgaaccgg cccggatgga
     1381 gaacgccagg gagtatgtca acgggctctt cttcgacacc cgcaggtata acctcggccg
     1441 cgtgggaagg tacaagctga accgccggct cggcctggat atcccggggt ctgtccgcct
     1501 gctcctggtg gacggcgtcg tgcagatagt taaaaaactg atgggcctca gggacggcta
     1561 cgagcggatc gacgacatcg accatctcgg caacaggagg gtgcgggccg tcggcgaact
     1621 gctccagaac caggtccgta tcggcctgct caggatggag aggatcgccc gggagaggat
     1681 gaccaccatc cccgacctca ccggcgccac cgcgagagaa ttgataaacg tccgtcccat
     1741 atcggcggcc ttgagggagt tctatggctc tggccagctt tcgcagttca tggaccagac
     1801 gaatcccctg gcggaactga cccatcggag aaggctctcg gcgctcggcc ccggcggcct
     1861 gagcagggag agggccggtt tcgaggccag ggacgtccac tacacccact acggcagggt
     1921 ctgccccatc gagactcccg agggtcccaa catcggcctg gtgacctccc tgtcgatcta
     1981 ctcgaggata aacgagtacg gcttcctcca ggcaccgaaa agaaaggtcg tcgacggcaa
     2041 ggtcaccgac gaagtcgtct acctcggggc agacgaggag gacgaatatt acatcggaag
     2101 ggccaacacg ccccttaact ccgacaggac cttcgcggaa aaggagatct acgtaaggga
     2161 caggggggat atcgccgtgg tcgagccggc caaggtcgac ttcctggata tttcgcccaa
     2221 gcagatcgtc tcggcctcga cggccctcat ccccttcctg gagcacgacg atgcaaaccg
     2281 ggctctcatg gggtccaata tgcagaggca ggccgtcccg ctgatcaact ccagggctcc
     2341 catcatcggc acggggatcg aggcgaggat agcccgggat tcgggctcct gcgtggtggc
     2401 gcgcaatccc ggcaccgtga tcggcgtcga ttcccggacg ataaagatcc aggccggcaa
     2461 ggccggcaag gtcgaccagt acaacctggt caagttcaag agatcgaacc agggtaccat
     2521 catccaccag aaacccatcg tcttcaaggg agacaaggtc aaggcggggg atatcatcgc
     2581 cgacggccag gcggtggaga acaccgaact ggccctgggc aacaacgtcc tcgtggcctt
     2641 cgtctcatgg gaggggtaca actacgagga tgccatcctg ctgagcgaca ggctggtgaa
     2701 gaacgacagc ttctcctctg tccatataga ggagtacgag gtggaggccc gcgacacgaa
     2761 gctcgggccc gaggagataa cccgggatat ccccaacgtc ggcgaggacg ctctcaagga
     2821 cctcgacgag accggtatcg tccggatcgg ggcggaagtg gtggcgggcg acatcctcgt
     2881 cggcaaggtc acccccaagg gtgagtccga ccagtcgccc gaggaaaaac tcctgagggc
     2941 catcttcggc gagaaggcca gggaggtcag ggacacctcc atgaggatcc cccatggcga
     3001 gggcggtaag gtcgtggcgg tgaagcgcct cagccgttcc gagaacggtg atgacctgac
     3061 gccgggagtc aacgaggtcg tcaaggtcta cgtggcgaag ataaggaaga tctcggtggg
     3121 cgacaagatg gcggggcgcc acggcaacaa gggtgtcgtc tcgaggatcc tccccgagga
     3181 ggatatgccc taccttcccg acggtactcc catcgacgtg gtgctgaacc ccatcggggt
     3241 gccaagccgc atgaacctcg gccaggtgct ggagaccatc ctcggcttcg tggcctccca
     3301 gaacgactgg cacgtttcca ccgcagtatt tgagggggct accgaacagg agatcctgga
     3361 cctggttgag aagtcccgcc aggagggttt caaggacatg acgcccgacg ggcgggtgct
     3421 cctctacgac ggcaggaccg gcgaacccat gaggcggccc gtcacggtgg ggaccatgta
     3481 catgatgaag ctcatccacc tggtggacga caagatccat gcccggtcca taggccccta
     3541 cagcctgata acccagcagc ccctcggggg gaaggcccag ttcggcggcc agcgcttcgg
     3601 ggagatggaa gtctgggccc tggaaggtta cggggccgcc cacgtcctcc aggagatcct
     3661 gaccgtcaag tccgacgaca tcaggggcag gctcaagacc tacgagagaa tagtgaaggg
     3721 tctcaacctt acggaacacg gcgtccccga gagcttcagg gtcctcgtca aggaactgca
     3781 gggcctgggt cttgacgtgg agatcgccta cgaggacggc tccatgggag agctgctcat
     3841 ggatgaggac gaagagccgg cgctcctccc gagagtgagg acccaggaga agcgcgaagg
     3901 acccgcgggc cgcccggagg gagccctcga tgaccttttc cacgacgagg tgcccgttga
     3961 ggaagaccgg ggccaactga tctcggtcct gggtggagag agcggcgacg acccggaaga
     4021 gggggctgag gaatagatga accagagagg gatctccggg gtcaagatca agcttgcgag
     4081 ccccgaaagg atcagggaaa tgtccagcgg ggaggtcaag aagcctgaga ccatcaacta
     4141 caggaccctc aggccggaga aggacggcct cttctgcgag agaattttcg gtcccaccag
     4201 gagctttgag tgcgcctgcg ggaagtacaa aaggagcggc cccaagttca agggcgtagt
     4261 ctgcgatcgc tgcggcgttg agatagccga taaccgcgtc aggagggaac gcatggggca
     4321 catcgagctg gcggcgccgg tggtccatat ctggtacctc cggggtatac ccagccgatt
     4381 gagcctgctc ctgggcactg ccacgaagga cctcgagaag gtggtctact tcgcccccac
     4441 ccggaggcgg gaacc
//
LOCUS       DAOSRG010000074         4409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_17908_length_4409_cov_9.219339, whole genome shotgun sequence.
ACCESSION   DAOSRG010000074 DAOSRG010000000
VERSION     DAOSRG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17908_length_4409_cov_9.219339"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..2155
                     /locus_tag="PLM30_03765"
     CDS             485..2155
                     /locus_tag="PLM30_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54321.1"
                     /translation="MFPRRFLSLILLLGLILQGTAACADFEVSSVKGPHIKDLYMVIY
                     RDPDSQMLALQKGELDVLGDIARPSDVDRLASNQEVSLSISRGFHLFFMGFNLRKEPW
                     DDRALRQAAAMAVPSRQIVRDIFSGYSEPVDTYLPPVSPYYEPDVTVHEHDPAKARQI
                     LADAGWKWSPEGTLIPPGGDRPLKSVKLLSPTAQVAPTTAEISNRIAEALKSIGIPLS
                     VEPLDFSSMISRLDRHDFDLFVMAWSLSRDPDSLFAFFHSSMDVPGGYNIQGLHDPEL
                     DKVLEELRWAPDEETAGEAARKAQRMLSDISPWIPIYSRYMVAAVRNDWEGVVSTRST
                     SADNLWTLLNMRPLHGGERPVMWCLPEEPRSLNPLGTSSAYDWQVLTLVYEGLIAVNP
                     ETLGDLPWLATDWKVETVDGEGGPHTRLTFTLREGVRWQDGLPFTSGDVRKTLEYLKE
                     HEIPRYYDNVRDIERIDTPDDHTVIVTMGNVSYWHLHNIGGSPVFPAHILESVKDWET
                     WQPAREINPSLKELTCLVGTGPFVFREYRPGEFVHLVRNEDFWLLGGD"
     gene            2159..3136
                     /locus_tag="PLM30_03770"
     CDS             2159..3136
                     /locus_tag="PLM30_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54322.1"
                     /translation="MRSYWTKRVLGAAAVLLLVIAANFFLFRIMPGDVVSTIVDPRFS
                     PEAKERMREVFGLDLPLGRQFVIYVRRMLTFDLGISFLSQRPVWDELKSRIPNTVALL
                     GSALVLSSALGTWLGVKAARKRGGFTEKVVLLSGSVSFSFPSFFVQLVLLLLFAYTFP
                     LFPLRGSVSIPLPEAGAARALDYLWHLLLPAGSLVLLGFGSWAIYVRNLMVKTLGEDF
                     ILMARAKGLPEGRVLWNHAFRSALPPVLTLFLLALPGIVSGAVLTETVFSLHGVGKYL
                     LDSIMGHDYPAAGAAFYILGLLTIASNLAADAAYALADPRIRVGGRVQS"
     gene            3133..4326
                     /locus_tag="PLM30_03775"
     CDS             3133..4326
                     /locus_tag="PLM30_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54323.1"
                     /translation="MIKPPRWSFMAFLLILAVAVAGPPMLPGDPGRAVAKPFAPPAWS
                     GGDAPPPFSGAITPASREVTIDWRWDAPENLTLKGQLAFDEDTPARLVWTTAEGDSFT
                     LASFGGGRLWEVDLDARDMAFKTGLGIPPFASATSLLFPGRGVQTLSLEGPAPSGGSL
                     GLALPGGRWGLLGTDQRGRDVLSLFIVGVRVSLLVGIAATLLASLLGLSIGLTSGYLG
                     GAADGLIMRAVDILLSIPVLPILMVFVGIWGKGLWQLVLILGLFSWMGTARTVRSLAL
                     SLRESCYVENLRSLGAGPWYILYRHLLPEAMPVVLAAVSLGVPGAILAEAGLSFLGLS
                     DPRVISWGRMLHEAHGFGAFTAGAWWLIIPPGLGISLICLVFLDMGRAIEEMADPRLK
                     GGGGR"
BASE COUNT          703 a         1480 c         1330 g          896 t
ORIGIN      
        1 ggcgtttcat aaccagaaca cctccttctt gcactcacct ctcgttgaaa tgcgccaccc
       61 gttatgcggg aacgcgccct atgttttgaa gcaagaaatt tgttaggctg gcctatctta
      121 tgtccctctc tccgctttgt caaggggatg agtcttcttg atatcatttc atttggatac
      181 caagaaccca gtccctgggc cattcccccg ttgaccgttt acgagttcgg tcttccgatt
      241 tacgattctc ggtttttggt ttccgattcc cgatttccga ttcccgattt ccgatttccg
      301 attcacgatt tacggcactt acgattcacg attcccgatt cacgattcac gatttacgat
      361 ttacgattta cgattcccga ttcacggatt tcgccctttt cgtatacaaa tgctcaaatt
      421 caaggtctga ccccaatcaa gggctgtgct atgataacga gtatcttttt gggaggtgac
      481 agccatgttc ccaaggaggt ttttatcgct cattctttta ctgggcctca tcctgcaggg
      541 cacggccgct tgtgcggact tcgaggtctc ctccgtcaag ggcccccata tcaaggacct
      601 ttacatggtg atctaccggg accccgactc gcagatgctg gccctccaga agggtgagct
      661 ggacgtcctc ggtgacatcg cccgcccctc cgacgtggac cggctcgcct ccaaccagga
      721 ggtctccctc tccatttccc gagggttcca cctgttcttc atgggcttca acctgaggaa
      781 ggaaccgtgg gacgaccggg ccctccggca ggcggcggcc atggccgtcc cgtcaaggca
      841 gatcgtgagg gacatttttt ccgggtacag cgagcccgtc gacacctacc tcccgcctgt
      901 ctccccctac tatgagcccg acgtgacggt ccacgagcac gaccccgcca aggccaggca
      961 gatcctcgcc gacgcggggt ggaaatggtc gcccgagggc accctcatcc ctcccggcgg
     1021 cgaccggccc ttgaagtcgg tgaagctgct ttcccccacc gcgcaggtgg cccccaccac
     1081 ggcggagatc tcgaacagga tcgccgaagc cctcaagtcg atcggcatcc ccctgtcggt
     1141 ggagcccctg gacttctcca gcatgataag caggctcgac cgccacgatt tcgacctctt
     1201 cgtcatggcc tggagcctgt cgagggaccc cgacagcctt ttcgccttct tccactcatc
     1261 gatggacgtg ccgggaggct acaacatcca ggggctccat gaccccgaac tggacaaggt
     1321 cctcgaggag ctccgctggg cccccgacga agagaccgcc ggcgaggccg cccgaaaggc
     1381 ccagcggatg ctctcggata tatcgccctg gataccgatc tactcccgct acatggtggc
     1441 cgccgtcagg aacgattggg agggcgtggt gagcacccgg tcgacctcgg cggacaacct
     1501 ctggaccttg cttaacatga ggcctctcca cggaggggag aggcctgtca tgtggtgcct
     1561 cccggaggaa ccgaggtcgc tcaacccgct gggcacgagt tccgcctacg actggcaggt
     1621 gctgaccctc gtctacgaag gcctgatcgc cgtgaacccg gagaccctgg gggaccttcc
     1681 ctggctggcc acggactgga aggtggagac cgtggacggc gagggcgggc cccacacgag
     1741 gctgaccttc accctcaggg agggcgtccg ctggcaggac gggctcccct tcacctccgg
     1801 ggacgtgagg aagaccctgg agtacctgaa ggaacacgaa ataccccgtt actacgataa
     1861 cgtcagggat atcgagagga tcgacacccc cgacgaccac accgtgatcg tcacgatggg
     1921 gaacgtgtcc tactggcacc tccacaatat aggcggttca ccggtcttcc cggcccacat
     1981 cctcgaaagt gtcaaggatt gggagacctg gcagcctgcg agggagatca acccctctct
     2041 gaaggagctc acctgcctgg tcggtacggg ccccttcgtt ttcagggaat acaggcccgg
     2101 tgaattcgtc cacctcgtca ggaacgagga cttctggctc ctcgggggtg attaaccctt
     2161 gaggtcctac tggacgaaga gggtcctggg cgcggcggcc gtcctcctcc tggtcatagc
     2221 cgccaacttc ttcctcttca ggatcatgcc cggcgacgtg gtgtcgacca tcgtcgaccc
     2281 ccgcttctcc cccgaggcca aggaacggat gagggaggtc ttcgggctgg acctccccct
     2341 gggccggcag ttcgtgatct acgtccggag gatgctcacc ttcgatctcg gcatctcctt
     2401 cctctcccag cggcccgtct gggatgaact taaaagcagg atccccaaca cggtcgccct
     2461 cctcggttcg gccctggtcc tgtcgtcagc tctcggcacc tggctcggcg tcaaggcggc
     2521 gagaaaaaga ggcgggttca cggaaaaggt cgtgctcctg tcgggatcgg tgtccttttc
     2581 cttcccctcc ttcttcgtcc agctggtcct cctgctgctc ttcgcctata ccttccccct
     2641 gttcccgttg agggggagcg tctccatacc cctcccggag gcgggggcgg cgagggccct
     2701 ggattacctc tggcacctgc tgctgccggc ggggtcgctg gtgctcctcg gcttcggctc
     2761 ctgggccatc tatgtgagga acctgatggt gaagaccctg ggggaggact tcatcctcat
     2821 ggccagggcc aagggcctcc ccgaggggag agtcctctgg aaccacgctt tccggtccgc
     2881 cctgccgccg gtgctcaccc tgttcctcct ggccctcccc ggcatcgtct ccggggccgt
     2941 cctgaccgag acggtcttct ccctccacgg ggtcggaaag tacctcctcg attccatcat
     3001 gggccacgac tacccggcgg cgggagccgc cttttacatc ctggggctgc tgacgatcgc
     3061 ctccaacctg gccgccgacg ccgcctacgc cctggcggac ccgcggataa gggtcggggg
     3121 gagggtccaa agttgataaa accgccccgg tggagtttca tggccttcct gctgatcctg
     3181 gccgtcgccg tagccggccc cccaatgctc cccggtgacc cggggagggc cgtggccaaa
     3241 cccttcgccc cgcccgcctg gtccgggggg gacgcgcccc ctcccttttc cggcgccata
     3301 acccccgctt caagggaggt aaccatcgat tggaggtggg acgcccccga gaacctgacg
     3361 ctcaaggggc aactggcctt cgatgaggat accccggcga ggctggtctg gaccacggcg
     3421 gagggggatt ccttcaccct ggcctccttc gggggaggcc gcctatggga ggtggacctc
     3481 gacgccaggg atatggcttt caagacgggc ctcggcatac cgcctttcgc cagcgccacg
     3541 tccctgctct tcccaggcag gggtgtgcaa accctctcgc tggagggacc ggccccgtcg
     3601 ggcggctccc tgggcctggc cctgcccggc ggcaggtggg gcctgctcgg gaccgaccag
     3661 agggggaggg acgtcctctc ccttttcatc gtgggcgtca gggtctccct cctggtgggg
     3721 atcgcggcca ccctgctggc ctcgctgctg gggctctcca tcgggttgac gagcggttac
     3781 ctcgggggcg ccgccgacgg actgatcatg agggcggtgg acatcctcct gtccatcccg
     3841 gtcctcccga tattgatggt cttcgtgggg atatggggta aggggctctg gcaactggtg
     3901 ttgatcctgg gtctcttctc atggatgggt acggccagga ctgtccgctc cctggcgctc
     3961 tccctgaggg aatcctgcta cgtcgagaac ctccgctccc tgggggccgg cccctggtac
     4021 atcctttaca ggcacctgct gcccgaggcg atgccggtgg tgctcgccgc cgtgtccctc
     4081 ggcgtacccg gcgccatcct cgccgaggcg gggctctcct tcctggggct ttccgacccc
     4141 agggtgatct cctggggtag aatgctccac gaggcccacg gtttcggggc cttcacggcc
     4201 ggtgcctggt ggctcatcat cccgccgggg ctgggcatct cgctgatctg cctcgttttc
     4261 ctcgacatgg gacgggccat cgaggagatg gccgacccga ggctgaaggg gggcgggggg
     4321 cgatgatcgc gatccgggac ctgtcggtga cctacaggac cgccgccagg agcgtccccg
     4381 ctgtcagggg cgtctccctg gacgtgccc
//
LOCUS       DAOSRG010000075         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_17919_length_4406_cov_6.512066, whole genome shotgun sequence.
ACCESSION   DAOSRG010000075 DAOSRG010000000
VERSION     DAOSRG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17919_length_4406_cov_6.512066"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLM30_03780"
     CDS             <1..725
                     /locus_tag="PLM30_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR54324.1"
                     /translation="DHDELIPKISIQRQFANGNIFYLAASRVFAMPSFYEMYAEGTWT
                     AGNPDLKPEKGWSYEAGMKGPDQYFWSVGVFYTALEDKIKSVYDPEIFKYTYRNLADF
                     RTYGVEVSKSWKLAEKWVFSIDGAWQHPEEKQDPSSPWVRSYGIPEWEVGGALKYCSG
                     PWEVILDVSWAGNRAGDAGWYSWDAGDYILVDLGISWDSGDNRISLFCNNLLDEKYTY
                     NSAGWHYYGPERGFRLSWERRF"
     gene            871..1686
                     /locus_tag="PLM30_03785"
     CDS             871..1686
                     /locus_tag="PLM30_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOR54325.1"
                     /translation="MEEFIRAGDLVFIWAPPKGDSFIIKAAPGGRQDTRLGQMLHDDL
                     IGKPFGSWAYTNMGKSFCLLRPNIQEYSRRVRRMTQILYPKDTGFILMSMNIFPGALV
                     VECGTGSGSLTSVLAQFVGDEGRVVSYEKKEEHSLLARRNCEKWGVAHRVDFKIRDIA
                     DGFDERGADALFLDVPNPWDYLGGAKEALAQGNRLGILVPTMNQVGKVLDGLGENFFF
                     DIQVSEILLRYYKTDSRRIRPEDRMIGHTGYLVFASNVIGPPAPNGDGGDEPY"
     gene            1683..2234
                     /locus_tag="PLM30_03790"
     CDS             1683..2234
                     /locus_tag="PLM30_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOR54326.1"
                     /translation="MTHDILLHICCAPDATVPWQSLIGEGHSVVGFFYSSNIHPFSEF
                     KKRALDVETLSRVLGSGCIFGPYEPPRWLEAVRGHLLAPEGGERCALCFRAQLSAAAA
                     LAFSLGIGSLSTTLTISPHKDVAVIDRIGGEVCREYGLQWIFRVWRKKGGFQLSVERS
                     SRLRFYRQRYCGCIMSIRDEGSE"
     gene            2242..3228
                     /locus_tag="PLM30_03795"
     CDS             2242..3228
                     /locus_tag="PLM30_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOR54327.1"
                     /translation="MGSRVAASGKLAPEILEKAVLAYGGAERDEVLIGPAVGEDAAVI
                     RWPADRYLVVASDPIVGADRGAGRLLVVVNSNDIASKGGDPAFLISTLIFPTGSTPGQ
                     ITSLVEEIHEACLEAGIAVIGGHTEINDRYDHPVICATMMGMAEKVLTARDIKPGDAV
                     VMTKHVAIEGMCILANDRRELLRRCLSDQEIDGILQWSDLMSVLPESRILRKWALFMH
                     DPTEGGFLGGLGEIARLSGLGVSLDRGAVSIDRLTLSCSECLGFDPLRLISSGVLVSV
                     IPRKFLDEALVALEASGIACSVIGEMVQGEGDVPIETREELWALLADGRPLD"
     gene            3270..4349
                     /locus_tag="PLM30_03800"
     CDS             3270..4349
                     /locus_tag="PLM30_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOR54328.1"
                     /translation="MRRLMKSRGADAAVALVREGYNWETCFHLSGFRGSSCALVIFAD
                     AALLVTDGRYLEQASGQTDLTVVDQGRGSIMEALKKELSARPEIGSLGFQARRVTCDF
                     YDGLKGLGRELIDLTSEFSSIRRKKDPEEAHTIEEAARIASAAFKDTLSSFLSGMTER
                     VFAARMEYEMRLKGAEGGWGGQDFIVASGQKSALPHGKPEDKIIERAENVLVDFGARH
                     KGYVSDITRSFSWGEPPQWVLDAHELLSEAQSEAASLLEPGRPTSDIDAAARKVIERA
                     GLGDLFIHGLGHGIGLELHEPPTLSPRSDEVIAVGDVVTVEPGIYFPRKGGVRLEDDY
                     YISPEKTLCLTTSLERKIFRIDARS"
BASE COUNT          951 a         1161 c         1363 g          931 t
ORIGIN      
        1 acgatcacga tgagctgatc cccaagatca gtatacagag gcagtttgca aacggaaata
       61 tcttctacct tgctgcatca agagtttttg cgatgcccag cttttacgag atgtacgccg
      121 aaggcacctg gacggcgggc aatccggacc tgaagcccga aaagggctgg agctacgagg
      181 caggaatgaa gggcccggat cagtatttct ggagcgtggg ggttttttac acggctcttg
      241 aagacaagat caaatcggtc tacgatcccg aaatcttcaa atatacctat cgtaaccttg
      301 cggatttccg gacttacggg gttgaggtat cgaaaagctg gaagctggct gagaaatggg
      361 tcttttcgat cgacggcgcc tggcagcatc ccgaggagaa gcaagacccc tcgtcaccct
      421 gggtcaggag ctacggcata ccggaatggg aggtcggagg ggccttgaag tattgcagcg
      481 gcccctggga agtgatcctt gacgtatcgt gggcaggcaa ccgtgcaggg gatgcaggtt
      541 ggtactcttg ggatgccggg gattatatcc ttgtggatct cgggatctca tgggattcgg
      601 gagacaacag gatatccctt ttctgtaata atcttctgga cgagaaatac acctacaact
      661 cggccggctg gcactattac ggcccggagc gaggtttccg gctttcatgg gagagacgtt
      721 tctaggttga ttccgaaaaa agctgtatcg agaacggggg tgcaaggagc gctcccgttt
      781 ttttatgcct tacgcctgat gtacaataca cttcggcgaa gggggcacct aagagcaccc
      841 gatgcctcac cgatgccggg gagagagaat atggaagagt tcatcagggc cggcgacctg
      901 gtcttcatct gggctccgcc caagggcgac agctttatca tcaaggcggc ccccggcggc
      961 aggcaggata cgcgcctggg tcaaatgctg catgacgatc tcatcggaaa gcccttcggt
     1021 agctgggcct ataccaacat gggcaaatcc ttctgccttc tccgtcccaa tattcaggag
     1081 tacagccgta gggtcaggag gatgacccag atactatacc cgaaggatac ggggttcatc
     1141 ctgatgtcca tgaacatctt ccccggggcg ctggtcgtcg agtgcggcac cggttctgga
     1201 agcctgacct cggtcctggc ccaattcgtc ggcgatgaag gtagagtcgt ctcctacgag
     1261 aaaaaagagg agcattctct gctggcccgg cgcaactgcg agaagtgggg tgtcgctcac
     1321 cgcgtggatt tcaagatcag ggatatcgcc gatggtttcg acgagcgcgg agccgacgct
     1381 cttttcctgg atgtccccaa cccctgggat tacctcggcg gggcaaagga agcccttgcc
     1441 caggggaacc ggttgggcat cctggtcccg acgatgaacc aggttggaaa agtcttggat
     1501 ggcctcgggg aaaacttttt cttcgacatc caggtatcgg agatactcct caggtattac
     1561 aagaccgata gcaggaggat ccgccccgag gacaggatga tcggccatac aggatacctg
     1621 gtgttcgcct cgaacgttat cggtccccct gctcccaatg gtgatggagg cgatgaaccc
     1681 tattgaccca tgatatcctg ctgcatatat gctgtgcccc cgacgcgacc gtcccctggc
     1741 aaagcctcat cggagaaggt catagcgtgg tgggtttctt ttattcgtcc aatatccacc
     1801 ccttctccga gttcaaaaaa agggcgctgg atgtggaaac tctatcacgg gttctgggga
     1861 gcggttgcat ttttgggccc tacgaaccac cgcggtggct ggaagcagtg agaggccacc
     1921 tcctggcccc cgagggcggc gaaagatgcg ctctctgctt cagggcacaa ttgtcggccg
     1981 ccgccgccct ggccttttcc ctggggatag gttcgttatc gaccaccctt acgataagcc
     2041 ctcataagga tgtcgctgtc atagacagaa ttggcggcga ggtgtgccgg gaatacggtt
     2101 tgcaatggat cttccgtgtg tggagaaaaa aagggggatt ccaactctcc gttgaaagaa
     2161 gcagccgttt gcggttttac aggcaacgtt actgcggctg tatcatgagc atcagggatg
     2221 aaggatcgga gtgatgcgaa gatgggaagc agggtagccg cttcgggaaa actggcgccg
     2281 gagatactgg agaaagccgt cctggcctac ggcggggccg aacgggatga ggttcttata
     2341 ggacccgctg tcggtgagga tgcggccgtc atccggtggc ctgccgacag gtacctggtg
     2401 gtcgcctcgg acccaattgt cggtgccgac aggggagccg gaaggctctt ggtcgtcgtc
     2461 aattccaacg acatagcctc gaaaggaggc gacccggcct tcttgatctc caccctgatc
     2521 ttccccacgg ggtcgacgcc gggacagatc acttccctgg tggaagagat ccacgaagcg
     2581 tgcctggaag cgggaatcgc ggtgatagga gggcacacgg aaataaatga caggtatgat
     2641 caccctgtga tctgcgcgac catgatgggg atggcggaaa aggtcctcac cgcgcgggac
     2701 ataaaacctg gcgacgcggt cgtcatgact aagcatgtcg ccatagaggg gatgtgcatc
     2761 ctggccaatg accgcaggga attactccgc cgatgcctgt ccgaccagga gatcgatggt
     2821 attctccaat ggtcagacct gatgagcgtg ctcccggaat ccaggatcct gaggaagtgg
     2881 gccctattca tgcatgaccc caccgagggg ggattcctcg gtggccttgg ggaaatcgca
     2941 cgcctgagcg gcctgggagt ctccctggac aggggggcgg tcagcattga ccggctgacc
     3001 ctctcctgct cggaatgcct tggatttgac cccttgaggt tgatctcctc gggcgtcctg
     3061 gtgtcggtga taccccggaa attccttgat gaagcccttg tagccctgga agcttcgggg
     3121 attgcctgct cggtgattgg ggagatggtc cagggggaag gcgacgtccc catcgagacg
     3181 agagaggaac tctgggcgct cctggctgac gggaggcctc tcgattgaaa ggctcgaccc
     3241 agggacattt tgaagggagg ttggtatcct tgcggcgact catgaagtcc aggggcgccg
     3301 acgccgctgt agccctggtc cgggagggat acaactggga aacctgcttc catctctcag
     3361 gttttcgtgg ctcgagttgc gccctggtga ttttcgccga tgccgccctc ttggtgaccg
     3421 atgggagata cctggaacag gcttcagggc agacagacct caccgtcgtg gatcagggac
     3481 gcggttccat aatggaagcc ttgaaaaaag aattatcagc taggcccgag atcggcagtc
     3541 tcggcttcca ggcgcggcgg gtcacctgtg atttttacga tggccttaag gggctcggaa
     3601 gggaacttat cgacctgact tccgagtttt cttccatcag gaggaagaag gaccccgaag
     3661 aagctcatac catagaggag gcagcccgga tagcctcggc cgccttcaag gacacccttt
     3721 cgtctttcct ttcgggtatg acggagagag ttttcgcggc caggatggag tacgagatgc
     3781 gcctgaaagg ggccgagggc ggctggggtg gacaggattt tatagtcgcc tccggtcaaa
     3841 agagcgccct gccccatgga aaacccgagg ataaaatcat cgaaagggcg gaaaatgtgc
     3901 tcgtcgactt cggcgcaagg cacaagggtt atgtaagcga tataaccagg tctttctcct
     3961 ggggggagcc gccacaatgg gtcctggacg ctcacgaact gctttccgag gcgcaatcgg
     4021 aagccgcttc ccttcttgaa cctggaaggc cgacttccga tatcgatgcc gccgccagga
     4081 aggtgataga aagggcgggc ctcggtgacc tcttcatcca cggcctcggg cacggcatag
     4141 gccttgagct gcacgagccg ccgaccctgt cgccccgctc ggacgaggtg atcgccgtgg
     4201 gggatgtggt gaccgttgaa ccaggtatct attttccccg aaaaggcgga gtgaggctgg
     4261 aggatgatta ttacatttcc ccggaaaaga cattatgctt gactacgtct ctggagagaa
     4321 agatcttcag aatcgacgcc cgctcctgaa cctcttctgg tgccacttcc aggctgtctc
     4381 tatgatctcg tcgatgtccg tgtagc
//
LOCUS       DAOSRG010000076         4342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_18259_length_4342_cov_8.650338, whole genome shotgun sequence.
ACCESSION   DAOSRG010000076 DAOSRG010000000
VERSION     DAOSRG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18259_length_4342_cov_8.650338"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1191
                     /locus_tag="PLM30_03805"
     CDS             193..1191
                     /locus_tag="PLM30_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54329.1"
                     /translation="MFKTRLLSQEEVKKLVSMKDVIDAAEKTFRGMGDGTVVNPTKVG
                     LDLGETAEFPPYEGFMNAMPAYIGWLDSAGIKWAGGFLGERKKRGLPYITSLILLIDP
                     KIGNFRAVMDGAYITNMRTGAQTAVALKYLHPGKNLKLGLFGAGMQGHTQTMAIAELF
                     HIDEVHVYDISPEASRKYAEDMKDTVQGKIVIAKTPEETATSADVVVCVTQSKDKYVK
                     DAWIKPGGILFPMGSYQECEDAYILNADRIIVDHVGQCLHRGALKELSEAGKITEKNI
                     YATIGEIVAGKKKGPGPSSERTLCIPIGTGAMDIAVASIALKKAEEKGIGGSYEFA"
     gene            1232..2227
                     /locus_tag="PLM30_03810"
     CDS             1232..2227
                     /locus_tag="PLM30_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54330.1"
                     /translation="MTALLITQKEIGELVSMAEVIDAVEKTFRGMGEGSVINPTKVHL
                     DLGDGESGLPYHAGMNSMPAYIGWLESAGIKFIGGWEDNPKKGLPYISGMILLVDPRD
                     GRFLSVMDGTLVTALRTGAQTAVALKYLVSEPRNPVIALYGAGAQGRTQAEAIATAFK
                     VKEFRVFDIKEEASRIFAEEMTAKLGVPIRVMDRPEDAVKGAHAVVSVTHARNKFIKN
                     EMIGEGVTVCPLGSFRECEDELILSVDKIIVDHVGQTLHRGALKDVSESGRLVEEDLY
                     GTIGEVVAGKKKGRENSKERILCIPIGTGAMDVTVATLAYRKALEKGVGKEFPFI"
     gene            2291..3346
                     /gene="dctP"
                     /locus_tag="PLM30_03815"
     CDS             2291..3346
                     /gene="dctP"
                     /locus_tag="PLM30_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOR54331.1"
                     /translation="MNSRDKKKKKGGNGMRAKWLVLGTALLVAIMVTTAGTAVAAAQY
                     HWKIGHIRPTGTDVDKDVNWLVEKIKEESGGRIEIDIFPASQLGDYTVVQERVSMGAI
                     EMQLACLGTTVSKALNLTAAPYLATNYEEAEKLFGPEGVVYQVVNEVLDKKENIHLIS
                     GWPNYFGGIALGKEAPAPGDPNVPKNIKIRVPPIKSYELTADALGYMATPIPWAETFT
                     ALQTGIVNGAIGAGAEGYYADLRDLMKEYLVINDHFEMWFWYMNKDLWNKLSKEDQDL
                     LTKLGRELEAKRWKAAPEAQKANEKRLADFGVKVIYIKPEEFEVIVKKAEEKVWPALK
                     GDIGEEYFNRTMEVLKK"
     gene            3525..>4342
                     /locus_tag="PLM30_03820"
     CDS             3525..>4342
                     /locus_tag="PLM30_03820"
                     /inference="COORDINATES: protein motif:HMM:NF020896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOR54332.1"
                     /translation="MVVETCLEKEKKEGATVTEGPLKIRKVLVQDVRFGEETEIRGKT
                     LFLNPDDIRRELLEDKHLLSVDVKIARPGESTRIIPVKDILAPAVKVDGPGCVFPGFI
                     GNPARTIAGRGTTAFLEGVAVVTSGRLVNFQEGLIDMTGPGAEYSIFSRLVNVVLVLE
                     PVEGLGKHEHERVVREAGVKAAAFIGKAAATAEFSEEMVYANETIPEIHSKYPDLPKV
                     VYVRQVLAQGLLHDNFLYGLNAREGAIPLYMQATELFDGAIVSGNCAAPCHKHTT"
BASE COUNT         1155 a         1001 c         1293 g          893 t
ORIGIN      
        1 cccccgatga agtgatcgat gccctggtga acgtccagca attattgccg gtggaactca
       61 gaggttccac ccaggggagc ctgggggtga caaagactgc gatccgcctg aaggaagagt
      121 ggctctcgcg gtgcaggcac tgcaaggata attgtcgttg acataatcca aaggatatgg
      181 agggaggata acatgtttaa aacaagactg ttgagccagg aagaagtgaa gaaacttgtc
      241 tcgatgaagg atgtgatcga tgcggcggaa aaaaccttca gagggatggg tgatgggacg
      301 gtggtcaatc ccacgaaagt cggcctcgac cttggagaga cagcggaatt cccaccctat
      361 gagggattca tgaatgccat gcccgcttat atcggctggt tggactcggc ggggatcaag
      421 tgggcaggtg gttttcttgg tgagagaaag aaaaggggcc ttccttacat tacctccctg
      481 atcctcctca tcgatccgaa aatagggaat ttccgtgccg taatggatgg cgcctacatc
      541 accaacatga ggaccggagc ccaaacagcg gtagccctga aatacctcca ccccgggaaa
      601 aacttgaagt tggggctttt cggggccggg atgcaggggc atacccagac catggcaata
      661 gcggaacttt ttcatatcga cgaggtccac gtctacgata tttctccaga ggccagcagg
      721 aagtacgcgg aagacatgaa ggatacggtc caggggaaga tcgtcatcgc gaaaaccccg
      781 gaagagactg caaccagtgc cgacgtcgtc gtctgcgtga cccagtccaa agataagtac
      841 gttaaggacg cctggatcaa acccggtggg atcctctttc ccatgggttc gtaccaggag
      901 tgtgaagatg cctacatcct caacgccgac aggatcatcg tggaccatgt cggacaatgc
      961 ctgcacaggg gagcattaaa ggaactgagc gaagccggaa agataaccga aaagaacatc
     1021 tatgccacta taggagagat cgtggcaggg aaaaagaagg gtccgggacc gtcatcggaa
     1081 aggactcttt gcattcctat agggaccggg gcgatggaca tagcggttgc ttcgatcgcc
     1141 ctcaaaaaag ctgaagagaa agggatcgga ggaagctacg aattcgctta aagtattagt
     1201 tctgtaccgt atcttaatag ggggtgaaag gatgacagct ttactcatca cgcagaaaga
     1261 gatcggcgaa ctggtttcca tggctgaagt gatcgatgcg gtcgagaaaa ccttccgggg
     1321 tatgggggaa ggatcggtca tcaaccccac gaaggttcac ctggacctgg gggatggtga
     1381 aagcggcctt ccctatcatg ccggaatgaa ttcaatgccg gcttacatcg gttggcttga
     1441 gtcggcgggg attaagttca tagggggatg ggaagataat ccgaagaagg gattgcccta
     1501 cataagcggg atgatactcc tggttgaccc cagggatggt cgattcctgt ccgtcatgga
     1561 cggtactctc gtgacggctc tcagaacagg tgcccagacg gctgtcgccc taaaatacct
     1621 ggtatcagaa ccccggaacc ctgtaatagc cctttatgga gcaggggccc agggacggac
     1681 ccaggccgaa gccattgcta ccgctttcaa ggttaaggag ttccgggttt ttgatattaa
     1741 ggaagaagct tctaggattt ttgcagaaga aatgaccgcc aaactagggg tgcctataag
     1801 ggtaatggac aggccggaag atgctgtcaa gggagcccac gcggttgtgt cggtaacaca
     1861 tgcccggaac aaattcatca agaatgaaat gataggggaa ggtgtcaccg tctgccccct
     1921 gggttccttc agggaatgtg aggatgaact gatcctctcc gtcgacaaga tcatcgtgga
     1981 tcatgtcggc cagactttac acaggggagc gctcaaggat gtcagtgaat caggccgtct
     2041 cgtggaagag gacctgtacg ggaccatagg ggaagtcgtc gcaggaaaga agaaaggcag
     2101 ggaaaactca aaggaaagga tcctctgtat tcccataggt acaggggcga tggatgttac
     2161 cgtcgctacc ctggcttacc gaaaagctct tgaaaaaggc gtgggcaagg aatttccctt
     2221 tatttaggtc aggaaatttt tcttgacctg gtattgggga aatatcatcg gtgacaacgg
     2281 gatgttgccg gtgaacagca gggataaaaa gaaaaaaaag ggagggaatg gcatgagggc
     2341 gaaatggttg gttcttggga cagcgttgct ggttgccatc atggtcacaa cggctggcac
     2401 ggctgtcgcg gcggcacagt accattggaa gatcggtcac atcaggccta ccgggacgga
     2461 tgtcgacaag gacgttaact ggcttgtcga aaagatcaag gaagagtccg gcggacggat
     2521 cgagatcgac atttttcccg caagccaact gggggactac actgtcgtcc aggaaagagt
     2581 atcgatgggc gctatcgaga tgcagttggc ctgcctgggt acgacggttt ccaaggccct
     2641 gaatctcacg gccgcgccat acctggccac aaattacgaa gaggcggaga aactctttgg
     2701 cccggaaggt gtagtatacc aagtagtgaa tgaggttctt gacaagaaag agaacattca
     2761 tcttatctca ggatggccta actattttgg gggtatcgcg ctaggcaagg aagctccggc
     2821 tccaggagac cccaatgtcc ccaagaacat caagatccgc gtcccgccga tcaagtccta
     2881 tgaattgacc gcagatgctt tggggtacat ggccacgcct atcccctggg ccgagacttt
     2941 cacggccttg cagacgggca tagtcaacgg agcgatcggg gcgggggccg agggatacta
     3001 tgcagacctc agggatctca tgaaggagta ccttgtaatt aacgaccact ttgaaatgtg
     3061 gttctggtac atgaataagg acctctggaa caagctctcc aaggaggacc aggaccttct
     3121 cactaagcta ggccgcgaat tagaggccaa gaggtggaaa gccgctcctg aagctcaaaa
     3181 agcaaacgaa aaaaggcttg ccgattttgg tgtaaaggtt atctacatca agccggaaga
     3241 attcgaggtc attgttaaaa aggcggagga gaaagtttgg ccggcactca agggagatat
     3301 cggggaagag tactttaaca gaacgatgga agtgctgaag aaatagccta tcaacgttta
     3361 gtgcttcccg cttaaccgtt ctttgagcga tgggccgggg ggcgcaagtc ccccggcttt
     3421 ttccaaaaac cttgggaagg gctttcctct gctaatttgg aagaagagcg tttaaagata
     3481 ggcctcagaa ttcgaatgct gggtggtacg agagggtgaa taccatggtt gtcgaaacct
     3541 gtcttgaaaa agaaaagaag gaaggcgcta ctgtaacgga aggaccgttg aaaatccgga
     3601 aagtattggt acaggatgta cgatttggcg aagagacgga gatcaggggc aaaacacttt
     3661 tcctaaaccc tgatgatatt cgaagagaat tacttgagga taaacacctc ctttccgtcg
     3721 acgtgaagat cgccaggccc ggagagagca ccaggataat ccccgttaag gatattctgg
     3781 cgccggcggt taaagtggac ggtcccggtt gtgtcttccc gggctttata ggaaaccccg
     3841 ccaggacgat cgccgggagg ggcactacgg cgttcctcga aggcgtggcc gtggtgacct
     3901 cgggaaggct ggtcaacttc caggagggcc tgatcgacat gactggcccc ggggccgaat
     3961 attcgatctt ctccaggttg gtcaatgtgg tcctcgtcct tgaaccggtt gaagggcttg
     4021 ggaagcacga acatgagaga gtcgtcaggg aagccggtgt gaaggccgct gctttcatag
     4081 ggaaagccgc ggcaacggct gagttttccg aagagatggt ctatgccaac gaaaccatcc
     4141 ccgagatcca ctcgaaatac cccgacctgc cgaaagtggt atatgtcagg caggtcttgg
     4201 ctcaagggtt gctccatgac aatttccttt acggattgaa cgctagggaa ggtgcaatcc
     4261 ctctgtacat gcaggccacg gaactctttg acggggccat cgtcagcggg aactgcgccg
     4321 ccccctgtca taaacacacc ac
//
LOCUS       DAOSRG010000077         4295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_18520_length_4295_cov_9.754245, whole genome shotgun sequence.
ACCESSION   DAOSRG010000077 DAOSRG010000000
VERSION     DAOSRG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18520_length_4295_cov_9.754245"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLM30_03825"
     CDS             complement(<1..476)
                     /locus_tag="PLM30_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54333.1"
                     /translation="MRYRVVLRRRFKTGLLFEGVLPVLSVAAALVFAGGALSIFGVSP
                     VATYRAMFRGALGSGYGLSEVVVKTIPLVISGIAVALAFRMKIWNIGAEGQIYLGALA
                     TAAVVRYFPSGNQAVMLLTMTAGAALAGGAWGYAAGFMKSRWNVNEVITTLMLNYIG"
     gene            complement(485..2071)
                     /locus_tag="PLM30_03830"
     CDS             complement(485..2071)
                     /locus_tag="PLM30_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54334.1"
                     /translation="MQLSTVQQEPLVTMRGIEKVFVGVRANAEVDFDLDRGEVHTLLG
                     ENGAGKSTLMNILSGLYTPDSGSIAVKGLPQSFRSPRDAIRAGIGMVHQHFMLVPTLS
                     VWENVALGLEGNSILMDKDLIVKRIRDIQDRYGIHVDPMTPVWQISIGEQQRVAIIKA
                     LYRNAEILILDEPTSVLTPQEARALFETIRQITAEGHGVVFISHKLDEVMDISDRITV
                     LRKGRKVATLPVAEVTKESLAEMMVGRKVVFQVGKTLQNPGRVALEARDLVVRGDRGR
                     TRVDHFSLDVRQKELLGLAGVSGNGQEELCDALAGIRKIEAGSIKVDGYELAGRPIRE
                     FQKRGVSYIPADRMGTGLVRGMNLRENIALRKYWRKEFTRFRFFIHWENIAGRTGQLV
                     ERFGVVHPGLGYPARMLSGGNLQKLMLARELTDDPKVVIAVQPTWGLDVSATLFVRET
                     LLEQRDKGTGVLLVSDDLEEILSLSDRIAVIHNGRLMGLVEDPDSVTEEQLGLMMAGT
                     CLEEFRPHGAEAPFAPGKVV"
     gene            complement(2161..3243)
                     /locus_tag="PLM30_03835"
     CDS             complement(2161..3243)
                     /locus_tag="PLM30_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOR54335.1"
                     /translation="MKKLILMMLCVSMILGASAASAFKPVAAKDLKIGFVYVGPVGDG
                     GWTYMHDMGRVEMEEAFPGVESFFVESVAEGPDSVRVMETFIRNGAKLVFATSFGYMD
                     FVQEVAAKNPDVIFMHCSGYKNAPNVGNYFGRMYQARYLSGLVAGNMTKKNVIGFVAA
                     NPIPEVIRAINSFTVGARKVNPDVKVKVVWLFSWLDPGKEKEATLALIDAGADVIAMH
                     ADSGAAPQTAEEAGVYVVGYNNDMSHYAPTMHLTSPVWDWGMAYKYFVGKVIDGTWES
                     SNIWWGLKEGFVGLSPISKVVPDEVVAIVESEKAKIIEGGWDVFYGPVRNQKGEEVVK
                     SGEKMTDEEMLSMNWFVEGVEGEIPK"
     gene            complement(3341..4162)
                     /locus_tag="PLM30_03840"
     CDS             complement(3341..4162)
                     /locus_tag="PLM30_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOR54336.1"
                     /translation="MKETPFKLKGFSYPLTPEGKASIVGPFPWHYGTEYLNILYRTDP
                     DAIASYLPRPLEPAEEPGLAYVAFSRWWSVWEERKDMPWVLPERTQYMEAAIWVGCAF
                     EGIQGQICLHIWVDNDFSLARGWFMGFPKKLGQVSMTSYHPLNPGMPAMGPGTKLKGV
                     ASSHGEKLFEGEMETIEKISPGELHRPMGLPLFHIRHFPSIVKGAPPSVLELVSLGAE
                     DYRYGEDVWRGKGGLSFFSSEIEEHMPLAPKEVLGAYSFSTGYTFDGGKVLHSWV"
BASE COUNT          810 a         1376 c         1240 g          869 t
ORIGIN      
        1 ccgatatagt tgagcatgag ggtggttatc acctcgttca cgttccaccg cgacttcatg
       61 aacccggcgg cgtatcccca ggcgcctccg gccagggcag cgcccgccgt catggttaaa
      121 agcatgacgg cctggttccc cgatggaaaa taacgtacca cggcggccgt cgcgagggcc
      181 ccgaggtaga tctgcccctc ggcgccgata ttccagatct tcatcctgaa ggccagggcc
      241 acggctatcc cggagatgac caggggtatg gtcttgacca ccacctcgct aaggccgtag
      301 ccgctcccga gggcgccccg gaacatggcc ctgtaggtcg ccacgggcga tacaccgaat
      361 atcgagaggg cgcccccggc gaagaccagg gccgccgcga ccgacaggac cggaaggacc
      421 ccctcgaaga gcaaccccgt cttgaacctc cttctcagga ctacccggta cctcatagga
      481 aggatcagac cacctttcca ggagcgaagg gtgcttccgc gccgtgaggg cggaactctt
      541 cgaggcaggt tccagccatc atgagcccca gctgttcctc ggtgacgctg tcgggatcct
      601 ccacgaggcc catgagtctc ccgttgtgga tgaccgcaat acgatcgctg agtgagagga
      661 tctcctccag gtcgtcggag accagcagca cccccgtccc cttgtccctc tgctccagca
      721 gggtctccct gacgaagagc gtcgcgctga cgtcgagccc ccaggtgggt tgtacggcga
      781 tgaccacctt ggggtcgtcg gtgagctccc tggccagcat gagtttttgc aggttacccc
      841 ctgagagcat tcgggccggg tagcccaggc cggggtggac aacgccgaat ctctccacca
      901 gttgacccgt gcgaccggcg atattttccc agtgaatgaa aaagcggaac cgggtgaact
      961 ccttcctcca gtatttcctg agggctatat tctccctgag gttcatcccc cggacgagtc
     1021 cggtccccat ccgatcggct ggtatgtaac tgacgcctct tttctggaat tcccttatgg
     1081 gccgcccggc taactcgtaa ccgtcgacct tgatgctccc cgcttctatc ttcctgatcc
     1141 ccgccagggc gtcgcacaac tcctcctggc cgttgccaga gacgcccgcc agccccagga
     1201 gttccttctg ccggacatcg agggaaaaat ggtccacccg ggtcctgcca cggtcacccc
     1261 tcacgacgag gtccctggct tccagcgcca ccctgccggg gttctggagg gtcttcccca
     1321 cctggaagac caccttcctc ccgaccatca tctcggccag cgattccttg gtgacctccg
     1381 ctacaggcag ggtggccacc ttccttcctt tgcggagcac ggtgatgcgg tccgatatat
     1441 ccatcacctc gtccagcttg tgggagatga agacgacgcc gtgcccctcg gcggtgatct
     1501 gccggatcgt ctcgaaaaga gccctggctt cctggggtgt cagcaccgag gtaggctcgt
     1561 cgaggatcaa aatttcggcg ttgcggtaga gggccttgat gatggccacc ctctgctgtt
     1621 ccccgatgga gatctgccag acgggggtca tggggtcgac gtggatgccg tacctgtcct
     1681 ggatatccct tatccttttg acgatgaggt ccttgtccat cagtatggag ttgccttcaa
     1741 gccccagggc cacgttctcc cagacggaga gggtcggcac gagcatgaag tgctggtgga
     1801 ccatgccgat gccggccctt atggcgtccc ggggactcct gaatgactgc ggcagtccct
     1861 tgacggctat ggagccggag tcgggggtgt aaaggccgct gaggatgttc atcagggtgc
     1921 ttttgcccgc gccgttctcg ccgaggaggg tgtggacctc acccctgtcc aggtcaaagt
     1981 ccacctcagc gttggccctg acaccgacaa agaccttttc tatccccctc atcgtcacga
     2041 ggggttcctg ctggaccgta gacaattgca tccctccgag ggtttatcct gaggaacgac
     2101 cctgggccgc ggggaaagaa aagggggcgc gcctcccgcg cccccccagt tggctattgc
     2161 ttatttaggg atctcgccct ctacgccttc cacgaaccag ttcatcgaga gcatttcctc
     2221 gtcggtcatc ttttcaccgc tcttgactac ctcttcgccc ttctggttcc ttacggggcc
     2281 gtaaaaaaca tcccaaccgc cctcgatgat ctttgccttc tccgattcga cgatggcgac
     2341 gacctcatcg gggacaacct tggagatcgg cgaaaggccg acgaaccctt ccttcaggcc
     2401 ccaccatata ttgctggatt cccaggttcc atcaatgacc ttacccacga agtacttgta
     2461 agccatgccc cagtcccaaa ccggcgaggt gaggtgcatc gtgggggcgt agtggctcat
     2521 gtcgttgttg taaccgacga cgtagacgcc tgcctcttcg gcggtctggg gcgccgcccc
     2581 agagtcggcg tgcatggcga tcacgtccgc cccggcgtcg ataagggcga gggtggcttc
     2641 cttttccttg ccgggatcga gccaggagaa gagccagacc accttgacct tgacatcggg
     2701 gttgaccttc ctggcgccca ccgtgaagga attgatggcc ctgatgacct cggggatcgg
     2761 gttggcggcc acgaagccga tgacattctt cttggtcata ttgccggcga cgagccccga
     2821 caggtacctg gcctggtaca tcctcccgaa gtagttgccc acgttcggcg cgtttttgta
     2881 gccggagcag tgcatgaata tcacgtcggg gttcttggcc gcgacttcct gaacgaagtc
     2941 catgtacccg aaggaagtgg cgaagaccag cttggcgccg ttcctgatga aggtctccat
     3001 gaccctcacg gagtcggggc cttcagcgac ggactcaacg aaaaagctct ccacccccgg
     3061 aaaggcttcc tccatctcca ccctgcccat atcgtgcatg taggtccacc cgccgtcgcc
     3121 gacaggtccc acgtagacga aaccgatctt caggtccttc gccgccacgg gcttaaaggc
     3181 ggaggccgcc gaggcgccca ggatcattga tacacagagc atcatcagga tgagcttttt
     3241 cattcccttc atacctcccc ggaataatgt gttttccctt cgggtgactc cttacattct
     3301 ggacaaaaga ggccggaact tcaactcctt tcgaggaagt tcatacccaa ctgtggagga
     3361 ccttgccccc gtcaaaggta taacccgtac tgaaggagta ggcgcccagg acctctttcg
     3421 gcgccagggg catgtgctcc tctatctccg aagagaagaa ggagagcccc cccttgccgc
     3481 gccagacgtc ctcgccgtac cggtaatcct cggcgcccag cgagaccagt tccaggacgg
     3541 agggcggagc gcccttcacg atcgagggga aatgcctgat gtggaaaagg ggcagcccca
     3601 tgggccggtg caactccccg ggcgagatct tctcaatggt ctccatctcg ccctcgaaga
     3661 gtttttcgcc gtgggatgat gccacgccct tcagctttgt ccccggcccc atggcgggca
     3721 tgccggggtt cagggggtgg taggatgtca tggagacctg cccgagtttc ttggggaagc
     3781 ccatgaacca tcccctggcg aggctgaaat cgttatctac ccaaatatgg agacagatct
     3841 gaccctggat tccctcgaag gcgcagccga cccatatcgc cgcctccatg tactgggtcc
     3901 tctcggggag cacccagggc atatccttcc tctcctccca gacggaccac cagcggctga
     3961 aggccacgta ggcgaggccg ggttcttccg ccggttccag cgggcggggc aggtaggagg
     4021 cgatagcgtc ggggtcggtc ctgtagagga tgttcaggta ctccgtcccg tagtgccagg
     4081 ggaaaggtcc tactatggag gctttgccct ccggagtgag ggggtaggaa aaacccttga
     4141 gtttgaaagg ggtctctttc accgcgaaac cctcctcttc gttccgtggc aaaaaacaaa
     4201 aaatggcgga gacgtgtggg aatcgaaccc accgaagccg gccgtaccga cccccaccgg
     4261 gtttgaagcc cggggcactc accagagcac actcg
//
LOCUS       DAOSRG010000078         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19091_length_4195_cov_7.753865, whole genome shotgun sequence.
ACCESSION   DAOSRG010000078 DAOSRG010000000
VERSION     DAOSRG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19091_length_4195_cov_7.753865"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLM30_03845"
     CDS             <1..996
                     /locus_tag="PLM30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR54337.1"
                     /translation="YIGCGERGNEMTEVLEEFPHLKDPHTGRTLMERMVLIANTSNMP
                     VAAREASIYLGMSIAEYFRDMGLDVALMADSTSRWAEALREISGRLGEMPGEEGYPAY
                     LGSRLAQYYERAGRVRVLGEPQRKGSITIFNAVSPAGGDFSEPVTQASLRLSGAFWAL
                     DKRLAEGRHFPSVNWKQSYSLYEKALEPFFLENLGEQWKDCASFLKSLLDRETELLSL
                     VQIVGRDALSEQDKWDLEMGEIVRVAFLQQNAYDPHDAFNTLEQQMRLSEILKDLQCL
                     VSEEIERGVLHQQVQGLGLLRQVLALRGAPAEDFEKRSHQWLEEVARSLTTSEGR"
     gene            998..>2160
                     /locus_tag="PLM30_03850"
     CDS             998..>2160
                     /locus_tag="PLM30_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR54338.1"
                     /translation="MDLYREGTRSISGITGPLLFVSNGRKAALGELVTIETSKGKRTG
                     QVLQIDGDICAIQVFEGTLGLNPGDCTVWFEKDVVKVRLGQVLMGQTLDGRGIPRGGK
                     ALPCFEKEVPITGSPLNPAMRAGPSDFVETGIASIDLLNTIVRGQKLPVFSGSGLPAN
                     EIAAQVVRQAHVPGNPEQFLVVFAAIGITEREARFFRDSFEESGALFNGVLVVNLAGD
                     SAVERLLAPRTGLTIAEYFAFDRGYDVLVVMTDMLHYCEALREVSAAREEAPGRRGFP
                     GYLYSDLASLYERAGCVKGLKGSITQIPIVSMPGDDMTHPVVDLSGYITEGQIVLDRG
                     LQARGVYPPINVLSSLSRLMNKGIGEGRTFTGHRQVADQLFASYARSREVSRL"
     assembly_gap    2161..2260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2261..2857
                     /locus_tag="PLM30_03855"
     CDS             <2261..2857
                     /locus_tag="PLM30_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOR54339.1"
                     /translation="LERKKEALFKEIEADRSTFRAMRKEVEGMLAFVSYAYALVRLFE
                     GESVKTLLLWGVEPRKVRIVRHTLMGCKYSQFYPEDKDRGRLLGGLLMDPALASLHVD
                     ELLETLVRIEPLLWRYINLKSKLDALELEFEKTRRKVNNLEQEMLPVLEAQERRIRLA
                     LAERERDEAHAVKMMLRKKTRSRGTSFSARRRERPGRD"
     gene            2977..3285
                     /locus_tag="PLM30_03860"
     CDS             2977..3285
                     /locus_tag="PLM30_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOR54340.1"
                     /translation="MNMEQMLKQAQKLQTEIGKIQEGLARERVEGVAGGGMVKVAATG
                     QGEIVGISIEREVIDPEEKEMLEDLLLAAINDASRKSKELAQSRLGRFGAGLGVPGLG
                     "
     gene            3296..3925
                     /gene="recR"
                     /locus_tag="PLM30_03865"
     CDS             3296..3925
                     /gene="recR"
                     /locus_tag="PLM30_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR54341.1"
                     /translation="MSISEPVERLMKLFSRFPGVGDKTARRFALFLLQQPGEFSDELA
                     STISLMRESTFSCSICGNLTTEDPCPVCSDPFRDRGVLCVLENVEDMLSLEQAGVFSG
                     LYFILGGRVSPMDGEDLPPKRLARLGEYVREGGFREVIIATNPSVEGDLTFYAVLDSL
                     KSMKPRITRLAFGLPLGGTIGYADRVTLHASIESRVDVSLANNSFERKE"
     gene            3930..>4195
                     /locus_tag="PLM30_03870"
     CDS             3930..>4195
                     /locus_tag="PLM30_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOR54342.1"
                     /translation="MAEGIFKLMLYLARAIFVFLGGVAGYQVVRIAVLQDWWPKGTFH
                     FSFIPFIFGVVLFALIGYLVTPFFVRLLGFIGLSFEKNLETLSW"
BASE COUNT          879 a         1132 c         1271 g          813 t
ORIGIN      
        1 tacataggct gcggcgagag gggcaacgaa atgacggagg tcctcgagga gttcccccac
       61 ctgaaagacc ctcatacggg cagaaccctc atggaaagga tggtcctcat cgccaacacc
      121 tccaacatgc ctgtcgcagc gagggaggcc agcatctacc tagggatgag catcgccgag
      181 tatttccgtg atatggggct tgacgttgcc ctgatggccg attccacctc tcgttgggcc
      241 gaggcgctga gggagatctc cggccgactg ggtgagatgc ccggcgagga aggctatccc
      301 gcttacctcg ggtccaggct cgcgcaatac tacgagaggg ccgggagagt gagggtcctc
      361 ggcgagcccc agcggaaggg gtcgatcacg atcttcaacg ccgtttcccc tgccggcggg
      421 gatttctcag aacccgtcac ccaggccagc ctgaggttat ccggggcctt ctgggccctc
      481 gacaagaggc ttgccgaggg ccggcatttc ccctcggtca actggaaaca gagctattcc
      541 ctctatgaaa aggccctgga gcctttcttc cttgaaaacc tcggcgagca atggaaggac
      601 tgcgcttcct ttctcaagag tctcctggac cgggagacgg agctgctctc actggtgcag
      661 atagtgggga gggatgccct ttcggaacag gacaagtggg acctggagat gggggagatc
      721 gtgagggtcg ccttccttca acagaacgcc tacgaccccc atgacgcttt caatacgctg
      781 gagcaacaga tgcgtttgag cgagatcctg aaggatctcc agtgcctggt gagtgaagag
      841 atcgaaagag gcgtcctgca ccagcaggtc caggggctgg gcttgttacg acaggtcctt
      901 gcgctgaggg gggcgccagc tgaggacttt gaaaagaggt ctcaccagtg gcttgaagaa
      961 gtggcccgat cgttgaccac ttcggaggga cgctgaaatg gacctttaca gggaaggcac
     1021 aaggtccatc agcgggataa cgggcccctt gctcttcgtt tcaaacggca ggaaggctgc
     1081 cctgggcgaa ctggtcacca tcgagaccag caagggaaaa cggaccggac aggtgctgca
     1141 gatcgacggc gacatctgcg cgatccaggt cttcgagggg acgttggggc tcaaccccgg
     1201 cgactgcacc gtatggttcg aaaaggacgt ggtcaaggta cggctgggac aggtcctgat
     1261 gggccagacc ctcgacggca ggggcatccc caggggaggg aaagcccttc cttgcttcga
     1321 gaaggaggta cccatcacgg gttctcccct caacccggcc atgagggccg gccccagcga
     1381 cttcgtcgag accggcatcg cctcaattga ccttcttaac acgatcgtga gagggcaaaa
     1441 acttcccgtt ttttccggat cggggctccc cgccaacgag atagccgccc aggtggtcag
     1501 acaggcccac gtcccgggaa acccggaaca gttcctggtc gtcttcgccg ccatcgggat
     1561 aaccgaaagg gaggcccgtt ttttcaggga ttccttcgag gagtcggggg cccttttcaa
     1621 cggcgtcctc gtggtcaacc tggctggaga ttccgccgtg gaacggctcc tggcgccccg
     1681 gaccggcctg actattgcgg aatacttcgc ctttgatcgc ggatatgacg tcctcgtggt
     1741 catgaccgac atgctccatt actgcgaagc cctcagggag gtgagcgccg cgagggagga
     1801 agcccccggg agaagggggt tccccggtta cctttactct gacctggcca gcctttatga
     1861 aagggccggc tgcgtaaagg ggctcaaggg cagcataacc cagatcccca tagtatccat
     1921 gcccggcgat gatatgaccc accccgtggt ggacctctcg ggctacataa cggagggtca
     1981 gatcgttctc gacaggggcc tgcaggcgag gggagtctac ccgccgatca acgtcctctc
     2041 aagcctcagc aggctcatga acaagggcat cggtgaggga aggaccttca ccggccaccg
     2101 ccaggtcgcg gaccagcttt tcgcctccta tgccaggtcc agggaggttt ccaggcttag
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggagagga agaaggaagc
     2281 cctcttcaaa gagatcgagg ccgaccggtc gaccttcagg gccatgagga aggaagtgga
     2341 gggcatgctg gccttcgtat cctacgccta cgccttggtc cgcctttttg agggtgaatc
     2401 ggtgaagacc ctgcttttgt ggggtgtcga acccaggaag gtcaggatcg taaggcatac
     2461 cctcatgggg tgcaagtatt cccagttcta tccagaggac aaggatagag ggcggctttt
     2521 aggcggcctg ctgatggacc ccgccctggc ctctttgcac gtggatgagc tcctcgaaac
     2581 cctcgtaagg atagaacccc tactgtggcg ttatatcaac ctcaagtcca agctggacgc
     2641 gctggaactg gagttcgaga agaccaggcg gaaggtgaac aaccttgagc aggagatgct
     2701 ccccgtgctg gaagcacagg agaggcggat aaggctggcc ctcgctgaaa gggaaaggga
     2761 cgaggcccat gccgtcaaga tgatgctcag gaaaaagaca agatcccgag gcacctcctt
     2821 ctcggcccgg cggcgcgaaa gacccggccg agactgacta taccctccct caccctacag
     2881 attaaaaacg ttccaggtcc tttgtaaaaa cgtcgttgag gagtaacatc aaacgatagc
     2941 ggaccgtttt tcggaaggaa ggatgacatc aaaacgatga atatggaaca gatgctcaag
     3001 caggcgcaga agctccagac agagatcggg aagatccagg agggattggc ccgggaaagg
     3061 gtcgagggag tggcgggggg tggcatggtc aaggtagcgg ccaccggcca gggcgagatc
     3121 gtcggcatct ccatcgaacg ggaggttatc gacccggaag aaaaggagat gctggaggac
     3181 cttctcctcg cggccatcaa tgacgcttcc cggaaaagca aggaactggc ccagtcgagg
     3241 ctcggcaggt tcggggccgg tcttggggtc cccgggctgg gttaggaggc cggtcttgtc
     3301 catttccgaa cccgtcgaaa ggttgatgaa gctcttttca cgcttccctg gggtgggtga
     3361 caagaccgcc aggaggttcg cccttttcct tctccagcag ccgggcgaat tctccgatga
     3421 attggcctcg accatttcac tcatgaggga gagcaccttt tcctgctcca tctgtggcaa
     3481 cttgaccacc gaggatccct gccccgtctg ctccgatccc ttccgggaca ggggcgttct
     3541 gtgtgtcctg gagaacgtcg aggatatgct ttcccttgaa caggccggag tcttttcagg
     3601 gctctacttc atcctcggag gcagggtctc gcccatggat ggcgaggatc tcccgccgaa
     3661 gagactggcc aggctggggg aatatgtacg ggaaggaggt ttcagggagg tcatcattgc
     3721 cacgaacccg agcgtcgagg gggacttgac cttttacgcc gtccttgact cgcttaagtc
     3781 gatgaagcca aggatcacca ggctagcctt tggattgccc ctcggaggga ccatcggtta
     3841 cgccgaccgg gtcactctgc acgcctcgat agagtcgagg gttgatgtaa gcctggcaaa
     3901 taattctttc gaaagaaagg agtgagaaca tggccgaagg aatattcaaa ctgatgcttt
     3961 acctggcaag ggctatcttc gtctttcttg gcggcgtggc gggctaccag gtggtcagga
     4021 tagctgttct gcaggactgg tggccgaaag ggaccttcca cttttccttt attcccttca
     4081 ttttcggggt agtgcttttt gccctgatag gttacctggt aaccccattt ttcgtgcgat
     4141 tactcggctt tataggatta tcctttgaaa agaaccttga aaccctcagt tggca
//
LOCUS       DAOSRG010000079         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19092_length_4195_cov_7.697826, whole genome shotgun sequence.
ACCESSION   DAOSRG010000079 DAOSRG010000000
VERSION     DAOSRG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19092_length_4195_cov_7.697826"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PLM30_03875"
     CDS             <1..1590
                     /locus_tag="PLM30_03875"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOR54343.1"
                     /translation="EDELEYYGPYKAKVTRGLWERIKSRPDGKLILVTAITPTPAGEG
                     KTTTTVGLGQGLSAIGKKAMIALREPSLGPSFGVKGGAAGGGYSQVLPMEDINLHFTG
                     DIHAVTTAHNLVSAMIDNHLQQGNELGIDPRRVTWRRVMDLNERALRNVVLGLGGKPH
                     GVPRESGFDITAASELMAILCLSSDLMDLKERVGRVIVGYTYDNKVIRAGDLGVAGAV
                     AVLLKDAIKPNLVQTLEGVPAFVHGGPFGNIAHGCNSIQATRHGLKLADYFVTEAGFG
                     AELGAEKFFDIKCRMAGLKPDAVVLVATVRALKMHGGVPKSELSREDVNALKSGLPNL
                     EKHIENVKAFGLPVVVALNRFPADTVAELDAVRERCDALGASFSLSEAWAKGGAGAVD
                     LAEKVVEACSGSNDFHYLYDAEDSPREKIETISRRLYGAGDVQYTDSALRDLAGIHEL
                     GLDHLLVCMAKTQASLSDDPAKIGRPDGFTLTVREVRISSGAGFLVPLTGSIMTMPGL
                     PKKPATVNIDIDAEGRITGLF"
     gene            1623..>2255
                     /locus_tag="PLM30_03880"
     CDS             1623..>2255
                     /locus_tag="PLM30_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOR54344.1"
                     /translation="MEQAKVIDGRGPAAELRASIAARVEELLAEGGKVPGLAAVLIGE
                     DPASRVYAGQKEKACREVGFKFSFHDLPAEAGTGMLVDLLGGLSEDPDVHGILVEFPV
                     PGHIDWATAVMAMSAEKDVDGVHPLNLGRLMMGLETVVPCTPRGAMYLLKKYGIDPAG
                     KRAVVLGRSNIVGKPMALLLQAADATVTVCHSRTTGLQSITREADIVVAAI"
     assembly_gap    2256..2355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2356..2753)
                     /locus_tag="PLM30_03885"
     CDS             complement(<2356..2753)
                     /locus_tag="PLM30_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOR54345.1"
                     /translation="MEESLSKWVSIIHRNLCSHMDRLFRDSDIGHGPRRFLAEIALTP
                     GLTQEEVSERLLMDKTTTARAVKQLENGGYITRTRDAADRRRYILNPTEKGGQVLPRV
                     LEAWRECHLALAEGFTDEELEVALAMLQRLA"
     gene            complement(2757..4103)
                     /locus_tag="PLM30_03890"
     CDS             complement(2757..4103)
                     /locus_tag="PLM30_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR54346.1"
                     /translation="MLTNEPVGGLIWRLSIPAMVAMFVNGLYNLVDTIYIGHGVGSMG
                     IAGLSIAFPIQMLIGGMGFMLGSGTASIISRSFGAKDYEKAHRALGNNLFSVMVLGLL
                     CMVLGRVFLTSILRLFGASEEILPYASEYMGVIFLGSPLTIFTMSMNNIIRSEGAART
                     AMWSMLVGAIANIILDPIFIFTFGMGIRGAAVATVLSRVFVLAWITWFYRSGGSFLSI
                     RAKHLAPRIALLKEIILIGFPALLQNASSSFVYGLINQILGFYGGNLALAVLGVNYRV
                     IMYSAMPAVGIAHGMQPIAGFNYGARRYGRVLEALRTSNIMALSLCSGIAITLLIFPG
                     PALRIFTSDKELLDAGMSAMRLMVAGTFFVGYNRVGGGFFQALGKALPAFVINSARPI
                     LFFLPLLVIMPRFLGINGIWLSFSLADILSFLLTAFLMAPETRFLRSLELAKEGAG"
BASE COUNT          896 a         1179 c         1286 g          734 t
ORIGIN      
        1 gaggatgaac tggaatacta cggcccttac aaggccaagg tcaccagggg gctttgggag
       61 aggataaaaa gtaggcccga cgggaagctg atcctcgtga ctgccataac ccccaccccg
      121 gcgggagagg gcaagacgac gaccaccgtc ggcctcgggc agggactctc ggcaataggc
      181 aagaaggcca tgatcgcctt gagagagccc agcctgggtc cgagcttcgg ggtcaagggc
      241 ggcgctgccg ggggcggcta ctcacaggtg ctccccatgg aggatatcaa cctgcacttc
      301 accggcgata tccacgccgt aacaacggcc cacaacctcg tatcggcgat gatcgacaac
      361 cacctccagc agggcaacga gctggggatc gacccgcgaa gggtcacctg gaggcgcgtg
      421 atggacctca acgagcgtgc cctccggaac gtggtcctcg gcctcggtgg gaagccccac
      481 ggggtcccca gggaaagcgg ttttgacata accgccgcct cggaactcat ggctatcctc
      541 tgcctttcct cggacctgat ggacctcaag gaacgggtag gaagggtcat tgtcgggtac
      601 acctacgaca acaaggtgat cagggccggt gaccttgggg tagcaggggc ggtggcggta
      661 ctgctgaagg atgccatcaa gcccaacctc gttcagaccc tcgagggcgt cccggctttc
      721 gtccacggcg gccccttcgg gaacatcgcc catggctgca acagcatcca ggccacccgg
      781 catgggctga agcttgccga ctacttcgtg accgaggccg ggttcggcgc cgaactgggc
      841 gcggagaagt tcttcgacat aaagtgccgg atggccggcc ttaaaccgga cgccgtggtg
      901 ctggtggcca cagtgagggc tttgaagatg catggtgggg tgccgaagag cgaactttcc
      961 agggaggatg tgaacgccct taagtcgggc cttcccaacc tggaaaagca tatcgagaac
     1021 gtcaaggcct tcggcctgcc ggtggtggtg gcgttgaacc gcttccctgc ggacaccgtg
     1081 gcggaactcg acgccgtgcg ggaaaggtgc gatgctctcg gggcgtcctt ctcgctttca
     1141 gaagcctggg ccaagggtgg cgccggcgcc gtcgacctgg cggagaaggt cgtcgaggcc
     1201 tgctcgggca gcaacgattt ccattacctc tatgatgctg aagactcccc cagggaaaag
     1261 atcgagacca tatcgcggcg gctttacggg gcgggggacg tgcagtacac ggactcagcc
     1321 ttgagggacc tcgccggcat acatgaactt gggctggacc acctgttggt gtgcatggcc
     1381 aaaacccagg cgtccctgtc ggatgacccg gcaaaaattg ggcggcccga cgggttcacc
     1441 ctgaccgtcc gggaggtccg catctcctcg ggggcgggct ttcttgtgcc cttgaccgga
     1501 agcatcatga ccatgccggg gttgcctaaa aagcccgcaa ccgtcaatat cgacatcgat
     1561 gctgagggca ggataacggg gctcttctag cgctcccgac aaaaaagggg gttgccgatc
     1621 ccatggaaca ggcgaaggtg atcgatggca gggggcccgc cgccgaactg agggcatcca
     1681 tagccgcccg cgtggaggaa ctgctggcgg aaggggggaa ggtccccggc ctcgccgccg
     1741 tattgatcgg cgaggatccc gcttccaggg tctacgcggg tcagaaggaa aaggcttgcc
     1801 gtgaagtggg tttcaagttc tcttttcacg atctgcccgc cgaggcgggc acgggcatgc
     1861 tcgttgacct gctgggcggg ctgagcgagg atcccgatgt ccacggtata ctcgtggagt
     1921 tccccgtccc ggggcacatc gattgggcta cggcggtcat ggccatgagc gcggaaaagg
     1981 acgtcgacgg ggtgcacccc ttgaaccttg gacgcttgat gatgggcctg gaaacggtcg
     2041 taccctgcac accccggggg gcgatgtacc ttctgaaaaa gtacggcatc gacccggctg
     2101 ggaagagggc ggtggtcctg gggaggagca acatcgtggg aaaacccatg gcgctgctct
     2161 tgcaggccgc cgatgccacc gtgacggtat gccacagcag gaccaccgga ctccaaagca
     2221 tcaccaggga agccgacatt gtcgtcgccg cgatannnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnngccag tcgttgaagc attgccaggg ccacctccag ttcctcgtcg
     2401 gtgaaaccct cggcaagggc caggtggcat tctctccagg cctcgagaac ccttggcagg
     2461 acctggccgc ccttttccgt ggggttgaga atatagcgcc tcctgtcagc ggcgtcccgg
     2521 gtgcgggtta tgtaaccccc attctcaagc tgtttcacag ccctcgccgt ggtggtcttg
     2581 tccatgagca gcctttcgct gacctcttcc tgggtgagac caggtgtaag ggctatttca
     2641 gcgaggaagc gccttggacc atggccgata tcgctgtcgc gaaaaagcct gtccatgtgc
     2701 gaacaaaggt tcctgtggat tatggatacc cacttactca gcgactcttc cattttttac
     2761 cccgccccct ccttggcgag ctcgagggat ctcaggaacc tcgtctcggg tgccatcaag
     2821 aaggcggtga ggaggaagga aagaatgtcg gccaaagaaa aggagagcca gatcccgttg
     2881 attccaagga aacggggcat gatgacgagg agaggcagaa agaacaggat gggtcttgcc
     2941 gaattgatca cgaaagccgg cagggctttc cccagggctt ggaaaaaacc gccgccgacc
     3001 ctgttgtagc ctacgaaaaa ggttccggcc accataagcc tcatcgcgga catacctgca
     3061 tcaaggagct ccttgtcgga ggtgaagatc ctcagggccg ggccggggaa gataagcagg
     3121 gtgatcgcga tccccgaaca cagggagagg gccatgatgt tggaggtcct taatgcctcg
     3181 agaaccctgc catatcgcct ggcaccataa ttgaagccgg cgatgggctg cattccgtgg
     3241 gctatgccga cggcgggcat ggccgaatac atgatcaccc tgtagttgac ccccaaaaca
     3301 gccagggcca agttcccgcc gtagaacccc aggatctggt tgataaggcc gtagacgaaa
     3361 ctggacgaag cgttctgcag caaggccggg aaaccgatca ggatgatctc cttcaagagc
     3421 gcgatacggg gggccaggtg cttcgccctt atcgaaagga aactaccccc ggaacggtag
     3481 aaccaggtga tccaggcgag gacgaagacc ctggagagga ccgtcgcaac ggcggcgccg
     3541 cggatgccca tcccaaaggt gaagatgaag atggggtcca ggatgatatt cgcgatcgcg
     3601 ccgacgagca tggaccacat ggccgtgcgg gccgcgccct cggagcgaat gatgttgttc
     3661 atcgacatgg tgaagatcgt gagtggactg cccaggaaga tcacacccat gtattccgag
     3721 gcgtagggca agatctcctc cgatgccccg aagagacgga ggatcgacgt gagaaatacc
     3781 ctgcccaaaa ccatgcataa aaggcccagg accatgacgg aaaacaaatt attgcccaaa
     3841 gccctgtggg ctttttcgta gtccttggcc ccgaagctcc tggaaatgat ggaagcggtc
     3901 cctgacccca gcatgaaacc cattccaccg atgagcatct ggatagggaa agctatcgaa
     3961 agcccggcga tacccatgga gcccacgcca tggcctatgt aaatggtgtc gaccaggttg
     4021 tacaggccgt tcacgaacat ggctaccatg gcggggatgg acagcctcca tatcaacccg
     4081 ccgacgggct cattggtcaa catgctttcc ctgctgttct tcaaagcaca cctccgctga
     4141 tttcaatcca aaccagcgga ttatagatca gtattgttgt atatgcaacc ttttt
//
LOCUS       DAOSRG010000080         4146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19367_length_4146_cov_10.959423, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000080 DAOSRG010000000
VERSION     DAOSRG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19367_length_4146_cov_10.959423"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLM30_03895"
     CDS             <1..540
                     /locus_tag="PLM30_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOR54347.1"
                     /translation="DEKALRGFTFLTQKPELVVLNLDETQVSADLPWYDDMEKLTSDR
                     GLGFAKVFGRMEMEMAELPPDEQAEFMAELEITEPGRERLIREAYSRLGLISFFTIGK
                     DEVKAWTLRKGGTALEAAGAIHTDLARGFIRAQVVHYDDFETSGFSNAVCREKGLLGL
                     EGKEYIVRDGDIIEIRFNV"
     gene            557..1564
                     /locus_tag="PLM30_03900"
     CDS             557..1564
                     /locus_tag="PLM30_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOR54348.1"
                     /translation="MTLLFEPLEIRGQRLRNRIVAPPMASLSCTNTGLPTMETLEYYK
                     PVSESGVGLAVLEHHAVHPEGRTRLRQLLLDRDEVLPYQQDLAGLFSGAGLPALVQVN
                     HAGSYVEDEDLLQNEWFPKAPSPIRHPRSTALIMPAKLTLEEIALVPVTFLEAAGRAA
                     RAGYAGVEIHAAHGYLLGQFLSPMTNRRTDNYGGSIKNRARLLFEVYEAVREAVGDEL
                     VVAVRLGMADTFPGADPVGQTIDDARWVASEFSSVGLDLLDLSGNMCGYDGHGEAWFA
                     PYCRTVKDAAGDIPTICTGGIRSAETALRLLDRGECDLVGIGRALKSDPNLVRKWKDL
                     E"
     gene            1561..2454
                     /locus_tag="PLM30_03905"
     CDS             1561..2454
                     /locus_tag="PLM30_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOR54349.1"
                     /translation="MRALGIDLGGHFIKGALIEDGSVAARNTVPTPGSRSPGDVIGAI
                     VALVTKLDPDGTEKTVGVGFPGMLDGRRERVLQAPNFPLMVNYPFRSALAQACGREVL
                     LENDANCAAKGEWAAGAARGLTDFAMLTLGTGIGGGIVSGGRLLTGAYGKAAELGHLA
                     VGGDAPCGCGALGHAEAVFGADALGRAFSLAGIKGSLPGLWLRRDEPTVATVWDRALD
                     ALARLIASLVHVLDPQAVILGGGLSRNPGLVETLEPRVLRYTATPFRDTLDIRFSSLG
                     DDAALLGSVLGSDLEFDHLYT"
     gene            2542..2757
                     /locus_tag="PLM30_03910"
     CDS             2542..2757
                     /locus_tag="PLM30_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR54350.1"
                     /translation="MAEKLPNFVKRKRKELGITQAELAERAGVGLRFLRELEQGKPTL
                     RLDKVNQLLDMFGFQMIPARKGTTDET"
     gene            2747..3082
                     /locus_tag="PLM30_03915"
     CDS             2747..3082
                     /locus_tag="PLM30_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOR54351.1"
                     /translation="MKRKAEITFFGTPAGILSEDDDGYRFTYYRSYLDNPDAMAISLT
                     LPLQKDPHFSAVLHPFFDGLIPEGWLLDVVERTWKVNPRDRMGLLMVSCKDCVGAVSV
                     IPAVEEKSE"
     gene            3079..4056
                     /locus_tag="PLM30_03920"
     CDS             3079..4056
                     /locus_tag="PLM30_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOR54352.1"
                     /translation="MKRKRICGYCYAALEDEGAFIHETCSLKFFGSRTPPVLPYSVSD
                     LDRLAEEAVRSRITVPGVQAKLSLHLAKGRGAPPRLTIVGPWGAFILKPPADEYPFLP
                     ETEDMTMHLASIAGIETVPHALMPLSGGRLAYITKRVDRLREKTARTGEMAKKHMEDL
                     CQLAEKPSEYKYIGSMELMSKLVRKYSSIPGYDLTRFFDLVLFSFLVGNGDMHLKNVS
                     LIYDDHSGIKLAPAYDLLSTRLIIPERVDPEEMALPVNGKKRGLDRRDFEAFARTSGL
                     NEKQAQNSFERITRKVPEMIAFLRKGFLPPEKAAEFTRLIQARNQRLQD"
BASE COUNT          877 a         1355 c         1184 g          730 t
ORIGIN      
        1 gacgaaaagg ccctgcgggg cttcaccttc ctcacccaga aaccggaact ggtggtcctc
       61 aacctcgacg agacccaggt ctcggcggac ctcccctggt atgacgatat ggagaagctc
      121 acctccgaca ggggactggg attcgccaag gtcttcggcc gcatggagat ggaaatggcc
      181 gaactccccc ccgatgaaca ggccgagttc atggccgaac tggagataac ggaacccggg
      241 agggagcggc tcatccgcga agcttactcc cggttgggac tcatcagctt cttcaccatc
      301 ggcaaggacg aggtcaaggc ctggaccctc cgcaagggtg gtaccgccct ggaggccgcc
      361 ggcgctatcc acaccgacct agccaggggc ttcatcaggg cccaggtggt ccactatgac
      421 gacttcgaaa ccagcggctt ctccaacgcc gtctgccgcg agaagggcct cttggggctg
      481 gaaggaaagg aatacatcgt ccgggacgga gatatcatag agataagatt caacgtctga
      541 tgggaggaat gccttcatga cccttctttt tgaacctctt gaaatccggg gccagcgcct
      601 gcggaacagg atcgtggctc cgcccatggc gtcgctctcg tgcaccaaca cggggctgcc
      661 cacgatggaa accctggaat actacaaacc cgtctccgaa agcggcgtcg gactggccgt
      721 tttggagcat cacgccgtcc atcccgaggg gagaacacgc cttcgccaac tactgctcga
      781 ccgcgacgag gtcctgccct atcagcaaga cctggcgggg cttttctccg gtgccgggct
      841 cccggcgctg gtccaggtga accacgccgg ttcctacgtg gaggacgagg accttctcca
      901 aaatgaatgg ttccccaagg ctccgtcacc cataaggcac ccccggtcca ccgccctcat
      961 aatgcccgcc aaactcaccc tcgaagagat cgccctggta ccggtgacct tcctcgaggc
     1021 cgccggcagg gccgccaggg cgggctacgc cggtgtggag atccacgcgg cccacggcta
     1081 cctcctgggc cagttcctga gccccatgac caaccggcgc accgacaact atggcggcag
     1141 catcaagaac agggccaggc tgctcttcga ggtctacgaa gcggtgcgcg aagccgtcgg
     1201 cgacgaactc gtcgtcgccg tcaggctggg gatggccgac accttccctg gggccgatcc
     1261 cgtggggcag accatcgacg atgcccgatg ggtggcgtcg gagttctcct ccgtcggcct
     1321 cgacctgctg gacctctcgg gcaacatgtg cggctacgac gggcacggcg aggcctggtt
     1381 cgccccctac tgccgcaccg tcaaggacgc cgccggcgac atccccacca tctgcaccgg
     1441 gggcatcagg tccgccgaga cggccctgcg gctcctggac cggggcgagt gcgacctcgt
     1501 aggcatcgga agggcgctca aaagcgaccc caacctggtc cgcaagtgga aggaccttga
     1561 atgagggccc tgggcatcga cctgggcggg cacttcataa aaggggccct catcgaggac
     1621 gggtcggtgg ccgcgaggaa caccgtcccc acccccggaa gccgttcccc cggtgacgta
     1681 atcggcgcca tcgtggccct ggtaaccaaa ctggaccccg acgggacgga gaaaaccgtc
     1741 ggcgtcggct tccccgggat gctcgacggc cgccgggaaa gggtcctcca ggcacccaac
     1801 tttcctctca tggtgaacta ccccttcaga tccgccctcg cccaggcatg cggccgggaa
     1861 gtcctgttgg aaaacgacgc caactgcgcc gccaaaggcg agtgggccgc cggcgccgcc
     1921 aggggactga cggacttcgc catgctcacc ttgggaacgg gcatcggcgg aggaatagtc
     1981 tccggtggac gacttcttac aggggcttac ggcaaggccg ccgaactggg gcacctggcc
     2041 gtgggcggcg atgccccctg cggctgcgga gcccttgggc acgccgaggc cgtcttcggc
     2101 gccgacgcct tgggcagggc cttctccctc gcggggataa agggcagcct ccccggactt
     2161 tggctcaggc gcgatgaacc tacagtcgcc accgtatggg accgggccct ggacgccctc
     2221 gccaggctca tcgcctccct ggtccacgtc cttgaccccc aggccgtcat ccttggcggg
     2281 ggcctcagcc gaaaccccgg cctcgtggag accctggagc cgcgagtgct gcgctacacc
     2341 gccacaccct tcagggacac cctcgacatc cgcttctcaa gcctcggcga cgacgccgcc
     2401 ctcctcggct ccgtcttggg gtcagacctt gaatttgatc atttgtatac ataaagataa
     2461 ggcttgacct caattgattt catacccgaa agggtgtaat gtcctttgac aaaggttttt
     2521 tataccctat aaggtgtaat tatggccgaa aaactaccaa atttcgttaa aagaaaaaga
     2581 aaagaactcg gaataaccca ggcggagctg gccgaaagag caggtgtcgg gctccgtttc
     2641 ctgagagagc tggaacaggg caagcccacc ctgcggcttg acaaggtcaa ccagttactt
     2701 gacatgttcg ggttccagat gatacccgcc aggaagggaa caacagatga aacgtaaggc
     2761 cgagataacc ttcttcggca ctcccgcggg catactttcg gaagatgatg acggctaccg
     2821 ctttacctac taccggtcgt acctggacaa ccccgacgct atggctatca gcctgaccct
     2881 ccccctgcaa aaggaccctc acttcagcgc cgtccttcac cccttcttcg acggcctgat
     2941 ccccgagggc tggctcctgg acgtggtcga aagaacatgg aaagtcaacc ctcgcgacag
     3001 gatgggcctc ctgatggtct cctgcaagga ctgcgtaggt gccgtatcgg tgatccctgc
     3061 cgtggaggag aaaagcgaat gaagaggaag aggatttgcg gctactgtta cgcagccctg
     3121 gaggacgaag gtgcttttat ccatgagacc tgcagcctta aattcttcgg ctccaggaca
     3181 ccgccggtcc ttccctactc cgtcagtgat ctcgaccgac tcgccgaaga agctgtgcgg
     3241 tctcggatca ccgtacccgg agtacaggct aaactgtctc tccacctggc gaaaggccgc
     3301 ggcgcgcccc ccaggctcac catcgtcggg ccctggggag ccttcatcct gaagccgcct
     3361 gccgatgaat acccttttct ccctgaaaca gaggacatga ccatgcacct ggcctccatc
     3421 gccggcatcg aaacggtacc ccacgccctt atgcctttaa gcggcggcag gctggcctac
     3481 ataacaaaaa gagtggacag gctgcgggaa aaaaccgcca ggaccgggga aatggccaaa
     3541 aagcacatgg aggacctctg ccagttggcg gaaaaaccgt cggagtacaa atacatcggg
     3601 tccatggagc tcatgtccaa actggtaaga aaatactcct ccataccggg ctacgacctc
     3661 accaggttct ttgacctggt cctcttctcc ttcctggtgg ggaacgggga catgcacctg
     3721 aaaaacgtat ccctcatcta cgacgaccat agcggcatca agctcgcgcc cgcctatgac
     3781 cttctctcga ccaggctcat catcccggaa agggtggacc ccgaagaaat ggcattacct
     3841 gtcaacggca agaagagagg gctcgatcgg agggatttcg aggccttcgc ccgcacctcc
     3901 gggctgaatg aaaagcaggc ccaaaactcc tttgaaagaa taacccgcaa ggtcccggaa
     3961 atgatcgctt tcctccggaa agggttcctc ccccccgaaa aggcggcgga attcacccga
     4021 cttatccagg cccgcaacca aaggctccaa gactgagccc caaaacaccg ccaccttggt
     4081 ttttggtttt ccgtcttaat tctcacgcac cttggccttt ccctatccct caaacctgaa
     4141 tctgtc
//
LOCUS       DAOSRG010000081         4146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19368_length_4146_cov_8.931802, whole genome shotgun sequence.
ACCESSION   DAOSRG010000081 DAOSRG010000000
VERSION     DAOSRG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19368_length_4146_cov_8.931802"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..>1063
                     /locus_tag="PLM30_03925"
     CDS             215..>1063
                     /locus_tag="PLM30_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR54353.1"
                     /translation="MRKEDLIDQAIEYTRRNRENVIGETDALSVSSRGVKIFDDPLFA
                     FADADDPLFFTLKEPRIIGGHYKLPKEWMAEAESVISFFLPFSEIIRSDNRRDNIWPS
                     AGWLHGRFEGQAFINGLCVFLQGLLSSAGFDSRIPSMESCFQSKSGPGDLQDENRSLE
                     NNLPFFTSNWSERHVAMVCGLGSFGLSKNLITIKGAAGRFGSLVTDLKIEPNPDRFES
                     FLSYCRSCGACIERCPANAISFEHGKDHFVCSKFLDRIKDHFRPRHGCGKCQVGVPCE
                     SSMPLIR"
     assembly_gap    1064..1163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1164..1999)
                     /locus_tag="PLM30_03930"
     CDS             complement(<1164..1999)
                     /locus_tag="PLM30_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOR54354.1"
                     /translation="MVLPLALVMGLGVLFRRLGLLDERTVHRLNSILYWAALPALLFR
                     SILKVDQDILSNPNLFWAAHATFLIGPALALFLGRAFTSDRRVLAVSALISIRSNNVF
                     LGIPAVMTVMGIEGLQAISLYLAVSLLGYNLLSIIWAQLVLSGKLTLRSLITTLGALL
                     KNPLILACLMGVAGSLAGFNKLPQWFDSALKMVGDSGSGIALIVLGATIRFENLGEAM
                     KSTWRDCLFKLFICPAIILCAFQIWPVNQVLRNTVVLTSAMPAATHNFVIAQSMEMDH
                     EYA"
     gene            complement(2106..2453)
                     /gene="rplS"
                     /locus_tag="PLM30_03935"
     CDS             complement(2106..2453)
                     /gene="rplS"
                     /locus_tag="PLM30_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR54355.1"
                     /translation="MDPRIALIESRFLRKETPDFRAGDTVKVHVKVTEGSRERIQVFE
                     GVVIARKHGGTRETFTVRKISGGVGVERVFPLHCPSVDHIEVVRHGKVRRAKLYYLRK
                     LSGKAARIKERRF"
     gene            complement(2490..3653)
                     /gene="trmD"
                     /locus_tag="PLM30_03940"
     CDS             complement(2490..3653)
                     /gene="trmD"
                     /locus_tag="PLM30_03940"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR54356.1"
                     /translation="MIVTVFTPFPELLEPFSFSGVIGKAVEKGLISIRCVNPRDHADG
                     HYGQIDDYAFGGGGMVLMAEPLARAVGSAGPREGLFVIHPGPQGVVFGQSLAETLATK
                     GHLVIVCGRYEGVDERFLEHFVDLEVSIGDYVLTGGELPAMVIIDAVARLIPGVVGQG
                     EAVEEDSFFRSMLDTPHYTRPATWRGVPVPEVLTDGDHGKTRSWRRRQSVTRTLARRP
                     DLIARANIGPYLDRGVYVILVPATGEVSRKEAEALGDLCGSFGCEKALLVVPVEEDRR
                     QLQGDPELKVKVVPSAESALEWVRKREKGEPFVIAFSDEEESSTHWLEAKRLALVSGR
                     PVVFLVGPGTGAGLEKIGTDVVLAPPRGGKDDKSLLRWQDRLVVVLDRFFGRR"
     gene            complement(3653..>4146)
                     /gene="rimM"
                     /locus_tag="PLM30_03945"
     CDS             complement(3653..>4146)
                     /gene="rimM"
                     /locus_tag="PLM30_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300370.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOR54357.1"
                     /translation="GAHGVRGEIKVLPLTDFPERFLGMARLEVFRPAGRLIASLTVKS
                     LRFHEAKGLFLVETGEIGDRDGAEALKGGVVMIKTSERVPLPEGSYWVDDVVGLRVVS
                     ADTGEEIGKVEAVLPTGAHDVYSVRTPQGEVRMMPAVREVVLRILPEEGIMEVRPLEG
                     LWD"
BASE COUNT          887 a         1209 c         1096 g          854 t
ORIGIN      
        1 ggggtgataa ccagcaggga ggcttcagcc tctttggggc tgtcggtgcg tcaggtccag
       61 ctcatgaagc ccgctcacac agacccatga ccaaatactc atggagtcac tgccgcaaag
      121 ttgtgactcc tcgggccgat cctgtgatat ctttgagccg tttgtttggc ccttcccatc
      181 ttttgggata agacaacttg gagggtgaca catcttgagg aaagaagatt tgatcgatca
      241 agcgattgag tatacaagga ggaacagaga aaatgtcatc ggtgaaactg atgctctatc
      301 ggtttcttca aggggggtca aaatttttga cgaccctctt ttcgcctttg ccgacgctga
      361 tgatcctcta ttctttactt taaaagaacc aagaatcatt gggggacact acaaacttcc
      421 taaagagtgg atggcggagg cagaatccgt aatctccttt ttcctccctt tcagcgagat
      481 catcagaagt gataacagaa gggacaatat ttggccttcg gcaggatggc tccatggaag
      541 atttgaaggc caggccttta ttaacgggtt atgcgtattc ttgcagggcc ttctatccag
      601 cgcaggcttc gattctagga tcccctcaat ggaaagctgc ttccagagta aatcgggtcc
      661 cggtgacctt caagatgaga acaggtcgtt agaaaataat ctcccttttt tcaccagtaa
      721 ttggtcggaa aggcatgttg caatggtatg cggcctgggc tccttcgggc tatcaaaaaa
      781 cctcatcact atcaaaggcg ccgcggggag gttcggaagt ctggttacag atttaaaaat
      841 cgaacctaac cctgatcgct tcgaaagctt tctatcttat tgccgttcct gtggcgcctg
      901 tattgaacga tgccctgcta atgcaatatc cttcgagcac gggaaagacc atttcgtctg
      961 ctcaaaattt cttgacagga tcaaggatca cttcaggcct cgacatggtt gcgggaaatg
     1021 tcaggtgggc gtgccttgtg agtcttccat gccgttaatt aggnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnngcgtatt catgatccat ttccatactt tgggcgatga
     1201 caaaattatg ggtggcagcc ggcatcgcgg aggtcagaac tacagtgttc cggagcacct
     1261 ggtttacggg ccagatctga aaagcgcaaa ggattatcgc tgggcatatg aatagtttga
     1321 aaagacaatc tctccaggtc gacttcatgg cctcgccaag gttctcaaat cgaatagtag
     1381 cgccaagcac gatgagggct atgccgctgc cggaatcccc gaccattttt aaagccgaat
     1441 cgaaccattg aggcaacttg ttgaaacctg cgagggatcc agctacgccc atcaaacagg
     1501 caaggatcag cgggttttta agcagggccc caagggtagt gatcaaggac ctcagggtca
     1561 acttgccgga aaggaccagt tgtgcccaaa tgatcgaaag gagattgtac cccagtaagg
     1621 agactgcgag gtaaagtgag atagcctgca acccctcgat tcccatcacg gtcatcaccg
     1681 ccggtatgcc caaaaaaacg ttatttgacc tgatggagat cagtgctgat acagctagca
     1741 ctctcctgtc gctagtaaag gctcttccca agaatagtgc taacgctggg cctattagga
     1801 aagtagcatg ggcagcccaa aaaaggttgg ggttcgagag gatatcctgg tccaccttca
     1861 ggatggaccg gaagagaagg gccggaaggg cggcccaata cagaatcgaa ttgagacggt
     1921 ggacggttct ctcgtcgagg agaccaaggc ggcgaaaaag aactccgagc cccatgacaa
     1981 gggccagggg taggaccatc aggaaagaag ccaaaatacc ctcccgttac cggaacatcc
     2041 cgggcaccga aggagacgat gcccgggtca aataggagcc cttggacggc tggtctgtgg
     2101 agtctctaga agcgtcgctc cttgatcctg gcagctttac cactgagctt cctcaggtag
     2161 tagagcttgg cccggcggac cttgccgtga cgtaccacct cgatgtggtc cacgctcggg
     2221 cagtggagcg ggaagaccct ctccaccccc acaccgcccg agatcttcct gacggtaaag
     2281 gtctcccggg ttccgccgtg cttcctcgcg atgacgaccc cttcgaagac ctgtatcctc
     2341 tccctgcttc cctcggtgac cttgacgtgg acctttaccg tgtcgccggc gcggaaatcg
     2401 ggagtttcct ttctcaggaa cctgctttct atcaaggcta tccttggatc cattgccatt
     2461 acctccctgt tgaatactac cgtgaaatgc taacgccggc cgaagaacct gtccagtacc
     2521 acgaccagcc tatcctgcca tctcaaaagg cttttatcgt ccttcccccc cctgggcggg
     2581 gcgaggacca cgtcggttcc aatcttctcg aggccagcac cggtcccggg accaaccagg
     2641 aagacgaccg gccttcccga gacgagggcg agcctcttgg cctcgagcca gtgggtcgag
     2701 ctctcctcct cgtcgctgaa ggcgataacg aagggttctc ccttctcacg ctttcttacc
     2761 cattccaggg cgctttcggc cgaggggacc accttgacct tcagctccgg atccccctgg
     2821 agttgcctgc ggtcttcctc caccgggacg accaggaggg ccttctcaca cccgaaggac
     2881 ccgcaaagat cgccgagggc ttccgcttct ttccgggaca cttcccccgt cgcggggaca
     2941 agtatgacat aaacacccct gtcaaggtag ggcccaatat tagccctcgc gataaggtca
     3001 ggtctcctgg ccagggtcct ggtgaccgac tgccggcggc gccaggaacg ggtcttcccg
     3061 tggtctccat cggtgaggac ttccgggacg gggacccctc tccacgtggc gggtctcgtg
     3121 taatggggcg tgtccagcat actcctgaaa aaggagtcct cctccacggc ttccccttgg
     3181 cctaccacgc ccgggatgag cctggccacg gcgtcgatga tcaccatggc gggcagctcc
     3241 ccgccggtca ggacatagtc ccctatcgat acctccaggt caacgaaatg ctcgaggaac
     3301 ctttcatcga cgccttcgta gcggccgcag acgatcacga ggtgcccctt cgtcgccagg
     3361 gtctccgcca gagactgccc gaagacgacg ccctggggtc ctggatggat gacgaagagc
     3421 ccctccctgg gcccggccga ccccacggcc ctggccagcg gttccgccat caggaccatg
     3481 cccccgccgc cgaaggcgta gtcgtcaatc tggccgtagt gaccgtcggc atggtcgcgg
     3541 gggtttacgc agcggatgct gataagcccc ttttctacgg ccttccctat gacgccggag
     3601 aaggaaaagg gctccagcag ttcggggaaa ggagtgaaca ccgtcacgat catcagtccc
     3661 agagcccctc cagcggcctg acttccatga tcccctcttc cggcaggatc ctgagcacca
     3721 cttcgcgcac ggccggcatc atcctgacct caccctgggg cgtcctaacc gaatagacat
     3781 catgggcacc cgttgggagc accgcctcca ccttacctat ctcctcgccg gtgtcggccg
     3841 agacaaccct gaggcccacc acgtcatcga cccagtaact cccctccggc aagggcaccc
     3901 tttcggacgt tttgatcatc acgaccccgc ccttgagggc ctcggccccg tcacggtcgc
     3961 cgatctcccc cgtttccacg aggaagagcc ccttcgcctc gtggaacctc agggacttta
     4021 ccgtgagcga ggctatgagc cgccccgccg ggcggaagac ctccagtcgc gccatgccga
     4081 ggaaccgctc tgggaagtcc gtgagcggga ggaccttgat ctcgcccctc accccgtggg
     4141 ccccga
//
LOCUS       DAOSRG010000082         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19564_length_4113_cov_10.781912, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000082 DAOSRG010000000
VERSION     DAOSRG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19564_length_4113_cov_10.781912"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="rfbB"
                     /locus_tag="PLM30_03950"
     CDS             <1..828
                     /gene="rfbB"
                     /locus_tag="PLM30_03950"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOR54358.1"
                     /translation="AESHVDRSIDSPEEFIQTNIVGTYRLLSAALEYFMALDAEGRQR
                     FRFLHVSTDEVFGSLSAEGYFTESTPYDPRSPYSASKASSDHLARAFHHTYSLPVLVT
                     NCSNNYGPFQFPEKLIPLVINSAVRGKDLPVYGDGMNIRDWLYVEDHCEALKLVLEKG
                     TPGETYNIGGGSEKTNIEVVRTLCRILDELQPREDGTGYERQITFVPDRPGHDRRYAI
                     DASRIGKELGWGPRNTFESGIRSTARWYLENQEWVEDVLSGKYRMERLGSHKRGGSE"
     gene            825..1706
                     /gene="rfbA"
                     /locus_tag="PLM30_03955"
     CDS             825..1706
                     /gene="rfbA"
                     /locus_tag="PLM30_03955"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOR54359.1"
                     /translation="MIRKGIILAGGSGSRLYPVTLGASKQLLPVYDKPMIYYPLSVLM
                     LAGIRNILIITTPEDRSNFVRLLGDGGRWGLDFTYSVQPEPGGLAQAFLIGEEFITRD
                     PCALILGDNLFYGQGLTGLLEEAVRTEQGATIFGYPVTDPQRYGVVEFDDQGRVVSLE
                     EKPDKPRSNYAVVGLYFYDGTVVERARELKPSKRGELEITDLNRHYLEDGLLRAMIFG
                     RGYAWLDTGTHDSLLDASKFVSTIQNRQGFMIACPEEIAWRKGYIDTYQLRIIAKAMR
                     NSYGDYLSILPDQFAPG"
     gene            1727..2134
                     /locus_tag="PLM30_03960"
     CDS             1727..2134
                     /locus_tag="PLM30_03960"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOR54360.1"
                     /translation="MRIIQLQRIQTSTGDVFVFEGERFDFPIRRVYFTRNVKVGEVRG
                     HHAHRTLEQILVCPYGSVLVSIDDGRGNTVEHKLTEPEKALFVGPCQWHTMTWLDKDS
                     VLLVFASNRYDERDYIRDYEEFIQAVKERDECV"
     gene            2131..2787
                     /locus_tag="PLM30_03965"
     CDS             2131..2787
                     /locus_tag="PLM30_03965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOR54361.1"
                     /translation="MKKEIIVEDDMIRALARLLRAFVRSPRHCLKSLFIRLSLPFIPA
                     KVILGKNLTPGQKMRFLGKGKIIIGEGCLFGVRKGGGFWDSSCELQARYPDSELLIGN
                     DVWSNNGLTVVASKRVEIKEKTLIGRNVQISDSDAHGIHPGKRRSERGESRPVVIGEN
                     VWIGNNVIILKGATIGDNSIIGAGSVVTGKVFPPNAIIAGNPAKVIKRIDNPLGETNE
                     "
     gene            2780..3892
                     /locus_tag="PLM30_03970"
     CDS             2780..3892
                     /locus_tag="PLM30_03970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR54362.1"
                     /translation="MNNEYKVVFNRLDNLYKQHENEYQGAALRVLRSGWYIMGPELEA
                     FEYEFANYTGSHFCIGLNSGLDALTLAIRSLGIGPGDEVIVQANTYIATVLAITANRA
                     APIFVEPDEYHGIDRERIEKAITPRTKAIMAVHLYGQPCDMGAISEISQRVGIPIVED
                     CAQSHGATFQGKMTGTFGVLGCFSFYPTKNLGAFGDAGAVVTDDPALADKIKILRNYG
                     SRVKYHNEIEGVNSRLDEIQAALLRVRLTYLEEILSEREEIVRRYLEGITNPLIKLPK
                     VRPGSRHTWHQFVIQCDERDRLQAHLKEQGVQTQAHYPIPPHLSEAYASLGFPRGSFP
                     ITEKLAKNVLSLPAYNGIGKEETALIIEAVNGFTPG"
BASE COUNT         1028 a         1074 c         1135 g          876 t
ORIGIN      
        1 gccgaaagcc acgtggaccg ctccatcgac tcgccggaag agttcatcca gaccaacatc
       61 gttggtacct accgcctcct ttcggccgcc ctggaatact tcatggccct ggacgcggaa
      121 ggccggcaac gtttccggtt tctccatgtc tcgaccgacg aagtattcgg ttccctgagc
      181 gccgaggggt acttcaccga gagtacgccc tacgacccga ggtcgcccta ctcggcctcc
      241 aaggcctcgt cggaccacct cgcgagggcc ttccatcaca cctacagcct tcccgtcctc
      301 gtgacgaact gctccaacaa ctacggcccc ttccagttcc ccgagaaact catccccctc
      361 gtgatcaaca gcgccgtgag gggcaaagac ctccccgtct acggcgacgg aatgaacata
      421 agggactggc tttacgtgga agaccactgc gaggccctga aactcgtcct ggagaaggga
      481 acgcccggtg agacctacaa catcggcggc ggatcggaaa agaccaatat cgaagtagtc
      541 cgcaccctct gccggatcct cgatgaactc cagccgaggg aagacggcac cggctacgaa
      601 cgtcagatca ccttcgtgcc cgacaggcct ggccatgaca ggagatacgc catagacgcc
      661 tccaggatcg gaaaggaact gggttgggga cccaggaaca cctttgagtc gggtataaga
      721 agcaccgccc ggtggtacct ggaaaaccag gaatgggtcg aggacgtcct ttcggggaag
      781 taccggatgg agcgcctcgg ctcccataaa aggggtggat cggaatgatc aggaagggga
      841 tcatcctcgc ggggggctcc gggtccaggc tctacccggt gacgctgggt gccagcaaac
      901 agctgctccc cgtatacgac aagcccatga tctactatcc cctgtcggtg ctgatgctag
      961 ccggcataag gaatatcctc atcatcacca cccctgaaga ccggtctaac ttcgtccgcc
     1021 tcctcggcga cggcggccgg tgggggctgg acttcactta ctccgtccag cccgagccgg
     1081 gagggctcgc ccaggccttc ctgatagggg aggagttcat aactcgcgac ccctgcgccc
     1141 tcatactcgg ggacaaccta ttctacggcc aggggctgac gggcctcctc gaggaggctg
     1201 taaggaccga acagggggcc accatattcg gctatcccgt caccgacccc cagcgctacg
     1261 gtgtggtgga attcgacgac cagggtaggg tcgtctccct ggaggagaag ccggacaagc
     1321 caaggtccaa ctacgccgtc gtgggccttt acttctacga cggcaccgtg gtggaaaggg
     1381 ccagggagct caaaccctcc aagagagggg aactggagat caccgacctc aaccggcact
     1441 acctggagga tggccttttg agggccatga tctttggaag gggttacgcc tggttggaca
     1501 cggggaccca cgacagcctt ctggatgcct ccaaattcgt ctctaccatc cagaaccggc
     1561 agggcttcat gatcgcctgc cccgaggaga tagcctggag gaagggttac atagacacgt
     1621 accaactgag gataattgcc aaggcgatga ggaacagtta cggtgattac ctctcaatac
     1681 ttccggatca atttgccccg ggataattag gaagggttgt ttagccttgc ggataattca
     1741 acttcagagg atacaaactt ctaccggtga cgtttttgtg ttcgagggag aaaggtttga
     1801 tttcccgata cggagggtct acttcacgag gaacgtaaaa gttggagaag taaggggtca
     1861 ccatgctcat aggacgcttg agcagatttt ggtttgccct tacggttcag tattggtaag
     1921 cattgatgat ggaagaggga atacagttga acataaactg acggaacctg aaaaagccct
     1981 tttcgttggt ccttgccaat ggcatacgat gacatggctt gataaggaca gcgtgcttct
     2041 tgtatttgca tcaaaccgat acgatgagag agattatatc agggattatg aagaatttat
     2101 ccaagccgtt aaagaaagag atgagtgtgt gtgaaaaaag agataattgt tgaagacgat
     2161 atgataagag ccctcgcaag gctgttgagg gcatttgtca ggtctcccag acattgcctg
     2221 aagtcattat tcattagact aagtttgcct ttcatccctg cgaaggtgat cctggggaaa
     2281 aacctcaccc ccggacaaaa gatgaggttt ttaggaaaag ggaagatcat tatcggggaa
     2341 ggatgccttt tcggtgttcg gaaaggagga ggtttttggg attccagctg tgaattacag
     2401 gcaagatacc cggattcaga actccttatc ggcaatgatg tctggagcaa taatggtttg
     2461 accgttgttg cttccaaaag ggttgaaatt aaagaaaaaa ctcttatcgg aagaaatgtt
     2521 cagatctcag attcagacgc acacggtatt catccgggaa aaagaaggag cgaacggggg
     2581 gaatctcgtc ctgtggtcat aggggaaaat gtttggatag gcaataatgt gattatcctg
     2641 aagggtgcga ccataggaga caattcgata attggagcgg gatcggtcgt aacaggaaag
     2701 gtgttccctc ccaatgcgat catcgcagga aatcctgcga aagtcattaa gcggatagac
     2761 aatccattag gcgaaaccaa tgaataatga atacaaggtc gtcttcaacc gtttagacaa
     2821 tctttacaag caacatgaaa atgaatacca gggagcagct ctgcgggttc ttcgttccgg
     2881 ctggtacatt atggggccgg aactggaggc atttgagtat gagtttgcca attacactgg
     2941 aagccacttc tgtataggcc ttaactccgg actcgatgca ttgaccttgg ccatcaggtc
     3001 cctggggata ggacccggcg acgaggtgat agtccaggct aacacctaca tcgctactgt
     3061 cctggcgatc accgccaata gggctgcgcc gatattcgtg gagcccgacg aataccacgg
     3121 catagaccgc gaaaggatcg aaaaggccat tactcccagg acaaaagcga tcatggccgt
     3181 ccatctctac ggtcagccct gtgacatggg ggccatctca gagatctcgc agagggtggg
     3241 catccctatt gtagaagatt gcgcccagtc ccacggagcg actttccagg ggaaaatgac
     3301 agggactttt ggggtcctgg gctgcttttc cttctacccc accaagaacc tgggggcctt
     3361 cggggatgcc ggggctgtcg ttaccgatga tcccgccctt gccgataaga taaaaatcct
     3421 gagaaattac ggaagcaggg ttaaatacca caacgagata gagggggtca actcgcgcct
     3481 ggacgagatc caggcggctc tcctgcgtgt caggctcacc taccttgaag agattctgtc
     3541 cgagcgtgag gagatagtcc ggcgatacct cgaaggaatt accaacccat tgataaagct
     3601 ccctaaggtc cgcccggggt cgaggcatac ctggcaccag ttcgtgatcc aatgtgatga
     3661 aagggacagg ctccaggccc acctgaaaga gcagggggtc cagacccagg cccattaccc
     3721 gatccctccc cacctttcag aagcttatgc ctctttgggt ttccccaggg gttccttccc
     3781 gataacggaa aagttggcga agaatgtgct gagcctgccc gcttataatg ggatcggcaa
     3841 agaggagacc gccttaatca ttgaggccgt caacgggttc acccccgggt aaaggccatt
     3901 atggctggcg gcagggcaag gctttttatc gaaaatttct taatatatgg atcggggaac
     3961 gttcttacaa aagccatccc tttcctcatg ctcccagttc ttactcgcct gataagggat
     4021 cctgccgttt ttggggtctt tgatatcttc aacatcatca tacagttcgc aacccctggc
     4081 gatttctaga agatatgaac cctgtaaaaa taa
//
LOCUS       DAOSRG010000083         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19634_length_4103_cov_15.384634, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000083 DAOSRG010000000
VERSION     DAOSRG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19634_length_4103_cov_15.384634"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLM30_03975"
     CDS             <1..899
                     /locus_tag="PLM30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR54363.1"
                     /translation="LVVSAADGPMPQTREHVLLARQVNVPALVVFMNKVDMVDDPELL
                     DLVEMEIRDLLGKYDFPGDDTPIIRGSALKALESDDLGRDSQWAGAIWELMDACDSFI
                     PEPVREVDRPFLMPIEDVFTITGRGTVVTGRVERGIIKAGDEVAIIGMQEDIKKTVAT
                     SLEMFRKILDDARAGDNVGVLLRGVGKEDVERGQVLAKPGSITPHKHFKSEVYVLKKE
                     EGGRHTPFFSGYKPQFYFRTTDVTGDIKLPEGVEMVMPGDNATFEVKLIQPIAMDQGL
                     RFAVREGGRTVGAGVVTEILGS"
     gene            935..1084
                     /gene="rpmG"
                     /locus_tag="PLM30_03980"
     CDS             935..1084
                     /gene="rpmG"
                     /locus_tag="PLM30_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOR54364.1"
                     /translation="MADQIGLQCTQCKRRNYVTTVNKKKQQKKLEKMKYCKWCKAHTL
                     HKETK"
     gene            1106..1181
                     /locus_tag="PLM30_03985"
     tRNA            1106..1181
                     /locus_tag="PLM30_03985"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1139..1141,aa:Trp,seq:cca)
     gene            1213..1392
                     /gene="secE"
                     /locus_tag="PLM30_03990"
     CDS             1213..1392
                     /gene="secE"
                     /locus_tag="PLM30_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOR54365.1"
                     /translation="MKWKSFIREARAELKRVTWPSRQQVWYSTLVVVAVSLLVAAYLG
                     IVDVLLTAVFSRVIR"
     gene            1480..2022
                     /gene="nusG"
                     /locus_tag="PLM30_03995"
     CDS             1480..2022
                     /gene="nusG"
                     /locus_tag="PLM30_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR54366.1"
                     /translation="MIDKTERHWYIIQTYSGYENRVKANLQQRIATMGMDEKIFHVLV
                     PIEERVTVKDGKSKRTKRKVFPSYVLVDMVLDDQSWYVVRHTPGVTGFVGAGSYPIPL
                     TEREVSEIMSKIGKEQIKPKIEIDLKPGDVVRVKAGPFEGQVGPVTEVLEDKGKVKFS
                     VVVFGRDTLVEADYTDLEKI"
     gene            2085..2507
                     /gene="rplK"
                     /locus_tag="PLM30_04000"
     CDS             2085..2507
                     /gene="rplK"
                     /locus_tag="PLM30_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR54367.1"
                     /translation="MAKKVIAQIKLQLPAGKATPAPPVGPALGQHGVNIMEFCKQFNA
                     KTADQPGMIIPAVLTVYADRSFTFILKTPPASVLLKKAASLEKGSGEPNKVKVGKVTL
                     DQVREIAELKKVDLNANDVEAAMEMIKGTAVSMGIEVV"
     gene            2590..3300
                     /gene="rplA"
                     /locus_tag="PLM30_04005"
     CDS             2590..3300
                     /gene="rplA"
                     /locus_tag="PLM30_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR54368.1"
                     /translation="MKKRSKRYRAIAEKAPREPLGSLKEAIEQARENATAKFDESMEM
                     HVRLGVDPRHADQQVRSTIVLPHGIGVVKRVLVIAGGEKVKEAQEAGADLVGGEDMVQ
                     KIEGGWLDFDAVIATPDMMKSVGKLGKILGPRGLMPSAKTGTVTFGVGNAVSEIKAGK
                     VEFRVDKFGIIHNAFGKAGFPVENLYENGKALLRAIVRAKPAAAKGQYIKSLAVTSTM
                     GTSFRVDPNAAVKELSAE"
     misc_feature    3336..3484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3495..4028
                     /gene="rplJ"
                     /locus_tag="PLM30_04010"
     CDS             3495..4028
                     /gene="rplJ"
                     /locus_tag="PLM30_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR54369.1"
                     /translation="MPSQLKIEQVESLREKLQEAEAVFICEYRGLNVAKITALRASIR
                     GAGGEMNVAKNTLMGIALREESMPVPDSLMTGPNSFTIAYSDAAAVAKVIRDFSREKG
                     NEALVIKGAVLGDDILSMEQVSALADLPSREVLLAQLAGTMIGPVRGLVTVLSGTTRG
                     LVTCLERIKEQKEEKAA"
BASE COUNT          930 a         1200 c         1323 g          650 t
ORIGIN      
        1 tcctggtggt ctccgcggcg gacggcccca tgccccagac gcgtgagcac gtactccttg
       61 cccgtcaggt caacgtaccg gcgctggtgg tcttcatgaa caaggtggac atggtggacg
      121 accctgaact attggacctg gtggagatgg agatccgcga cctcctgggc aagtacgact
      181 tcccgggcga cgacacgccc atcatccggg gatcggcgct taaggccctt gaaagcgacg
      241 accttggccg cgacagccag tgggcggggg ctatctggga actcatggac gcctgcgaca
      301 gcttcatccc cgagcctgtt cgtgaagtgg acaggccctt cctgatgccc atcgaggacg
      361 tcttcaccat aacgggccgc ggcaccgtgg tcacgggcag ggtggagcgg ggcatcatca
      421 aagccggcga cgaagtggcc atcataggca tgcaggaaga catcaagaag accgtagcca
      481 cgtcccttga aatgttccgg aagatcctcg acgacgcccg tgcgggcgac aacgtgggcg
      541 tgctccttcg gggcgtaggc aaggaagacg tggagcgtgg ccaggtcctg gcgaagccgg
      601 ggagcatcac gccccacaag cacttcaagt ccgaagttta cgtactgaag aaggaagagg
      661 gcggccgtca cacgccgttc ttcagcggtt acaagccgca attctacttc aggacgaccg
      721 acgtgacggg agacatcaag ctgcccgagg gcgttgagat ggtaatgcct ggcgacaacg
      781 cgaccttcga ggtgaaactg atccagccca tcgccatgga ccagggactt cgcttcgccg
      841 tccgcgaagg cggcaggacc gtcggcgccg gcgtcgtcac cgagatactc ggcagctgat
      901 ccaatccaac cagggagaag aggttcgaca ttttatggca gaccagatag ggctccagtg
      961 cacccagtgc aagcgccgca actacgtgac caccgtcaac aagaagaagc agcagaagaa
     1021 gctggagaaa atgaagtact gcaagtggtg caaggcccat accttgcaca aggaaacgaa
     1081 gtagtgtaga atacctttcg cgcgcaggtc cgtggctcaa ttggcagagc accggtctcc
     1141 aaaaccgggg gttgcaggtt cgagtcctgc cgggcctgcc attatattca cacccgggag
     1201 gccgaattct ttatgaagtg gaagagtttc atccgcgagg cgagagcaga actgaaaagg
     1261 gtcacctggc ccagccgcca gcaggtatgg tattcgaccc tggtcgtcgt cgcggtgtcc
     1321 cttctggtag cggcctacct cgggatcgtc gacgtgcttc tgacggcggt tttctccagg
     1381 gtgatccggt agggtaaccc tggcctgtgg gttggaataa acgtacagga gacacttcct
     1441 cgggaggaca gggggccggg tagggccccc ttttaggtga tgatcgacaa gacagagagg
     1501 cactggtaca taatccagac ctattccggg tacgagaaca gggtcaaggc caacctccag
     1561 cagcgcatcg ccacgatggg catggatgag aagattttcc atgtcctcgt gcccatcgag
     1621 gagagggtca ccgtcaagga cggcaagagc aagcgcacca agaggaaggt cttccccagc
     1681 tacgtcctgg tggacatggt cctcgacgac cagtcctggt acgtcgttcg tcacacgccc
     1741 ggcgtcacgg gtttcgttgg ggccgggagc taccccatac cgctcaccga gcgcgaggtg
     1801 agcgagatca tgagcaagat cggcaaggag cagatcaagc cgaagatcga gatcgacctg
     1861 aaacccggcg acgtggtgcg ggtcaaggcg ggccccttcg aaggccaggt gggccccgtc
     1921 accgaggtcc tggaggacaa gggcaaggtc aagttctccg tcgtggtctt cggccgcgac
     1981 accctggtgg aggccgacta caccgacctt gaaaagatct agggcatgac gcccttttta
     2041 tcgcccggcc gctgccgggc ccaagtagga gggataatca gctaatggca aagaaagtaa
     2101 tagcacagat caaactgcag ctgcccgcgg ggaaggcgac accggctccg cccgtagggc
     2161 cggccctggg ccagcatggc gttaacatca tggagttctg caagcagttc aacgccaaga
     2221 cggccgacca gccggggatg atcatcccgg cggtcctgac ggtctacgcc gacaggagct
     2281 tcaccttcat cctcaagacg cccccggcaa gcgtccttct caagaaggcc gcctcgctcg
     2341 aaaagggctc cggcgagccc aacaaggtca aggtgggcaa ggtcaccctt gaccaggtca
     2401 gggagatcgc cgaactgaaa aaggtcgacc tcaacgccaa cgacgtggag gccgccatgg
     2461 agatgatcaa gggcaccgcc gtatcgatgg gcatcgaggt agtctagcgc cggcggcttt
     2521 gaccggcgta tccggtggga gagcccctgt tgaggggttc gccaatacca cggggaggga
     2581 aaaagtgtca tgaaaaagag aagcaagagg taccgcgcca tcgccgagaa ggcgcccagg
     2641 gagcccctcg gcagcctgaa ggaggccatc gagcaggcaa gggagaacgc cacggcgaag
     2701 ttcgacgaga gcatggaaat gcacgtccgc ctgggcgtcg atccccggca cgccgaccag
     2761 caggtccgca gcaccatcgt gctcccccac gggataggcg tggtgaagcg ggtcctggtc
     2821 atcgccggcg gcgagaaggt caaggaggcc caggaggccg gcgccgacct cgtcggtgga
     2881 gaggacatgg tccagaagat cgagggcggc tggctcgact tcgacgccgt catcgccacg
     2941 ccggacatga tgaagtccgt gggcaaactg ggcaagatcc tcggcccccg cgggctcatg
     3001 cccagcgcca agacgggcac cgtcaccttc ggcgtgggca atgccgtcag cgagatcaag
     3061 gccgggaagg tcgagttcag ggtcgacaag ttcggcatca tccacaacgc cttcggcaag
     3121 gcgggtttcc ccgtcgagaa cctctacgag aacggcaagg ccctcctgcg ggccatcgtc
     3181 agggcgaagc cggccgcggc caaggggcag tacatcaaga gcctggccgt gacatcgacc
     3241 atggggacct ccttcagggt ggaccccaac gccgcggtga aggaactctc cgccgaatag
     3301 gcgcccccgg gttttttccg ggaaaaactg ttataattct gccgttccat cgaccgcaga
     3361 cagcgggtgc gcaccgatgc gcttaatgac ttgcccgccg aggtctggaa gagcctgacg
     3421 catgccacag ccatgtgtcc aggtctcccg ggcccggagg gtccgggagt ttttttatgc
     3481 gaggaggtga ataaatgcct tcgcaattga agatcgagca ggttgagtcg ctcagggaaa
     3541 aattgcagga agccgaggcc gttttcatat gcgagtacag ggggctgaac gtcgccaaga
     3601 tcacggccct cagggcctcc atccgcggcg ccggcggaga gatgaacgtg gccaagaaca
     3661 ccctcatggg aatagcactc cgggaggagt ccatgcccgt ccccgactcg ctcatgaccg
     3721 gccccaactc cttcaccatc gcctattccg acgcggcggc ggtggccaag gtcatcaggg
     3781 acttctccag ggagaagggc aatgaagccc tcgtcataaa gggagcagtc ctgggggacg
     3841 acatcctctc catggaacag gtcagtgccc tcgcggacct gccctcgagg gaggtactgc
     3901 tggcgcagct ggcggggacc atgatcggcc ccgtgagagg cctcgtcacg gtgctctccg
     3961 gcaccacccg cgggctcgtg acgtgcctgg aaaggataaa ggaacagaag gaagaaaaag
     4021 ccgcctgaaa aaccgacagg tgtagatgaa aaaggaggaa caggaaatga ccgcagccaa
     4081 aaaggcagca gaaggcgaag aag
//
LOCUS       DAOSRG010000084         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19636_length_4103_cov_10.083498, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000084 DAOSRG010000000
VERSION     DAOSRG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19636_length_4103_cov_10.083498"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLM30_04015"
     CDS             complement(<1..592)
                     /locus_tag="PLM30_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOR54370.1"
                     /translation="MKEKDETRICVIGAGIVGGAISEALVPRYPVTATRRSFDKLNGL
                     SEKGVKVTSDNRAAAEDADLVILSVKPAQVVPVLKEIAETVSGKTVVSFAAAISTDIL
                     RLAAPKAHFVRAMTNIAVRIHKGFTLFYPEPDFPAERLPLLERILMELGEIQQVDEQY
                     LDVLTAMSGSGPAYIFTVIESMIYGALREGLPRNLAL"
     gene            914..1123
                     /locus_tag="PLM30_04020"
     CDS             914..1123
                     /locus_tag="PLM30_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOR54371.1"
                     /translation="MARWLMVAGVVLLLLGAVLHLAPWLLSWFGKLPGDIRYETGRTR
                     VFIPITSMVVVSLVLTLVMNLLSRR"
     gene            complement(1142..2389)
                     /locus_tag="PLM30_04025"
     CDS             complement(1142..2389)
                     /locus_tag="PLM30_04025"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOR54372.1"
                     /translation="MKADLDRIRKDLENLALFNATPGKGLTRFSLTPEDRGARDYLSG
                     RLRELGVHVYEDAAASLFGRREGGDPSLPPIMIGSHFDSVRHGGNFDGQAGVVAALEV
                     LRCLEERGVRTAHPVEMIAMIEEEGGRFGAGFLSSRAMTRGVTREELEQRKDEDGVTL
                     AEALTRFGFDPARIAEARRKPGEVRAFLEMHIEQGPVLEREGFELGIVTSIVGTDLTR
                     VTVEGRPDHAGTTPMDMRRDALRTAAAVIGRVPSFAAEQGGDTVATVGSLVVRPGAAN
                     VVPGEVEFTLDIRSLDALSIKTVKDRVRRELDERSAADGTAYRAEQLLEVGPVELDEG
                     IRRVLQDQADGLGFSWRAMSSGAGHDAMIMAPFFPTGMIFVPSRGGRSHCPEEWTDFE
                     DLQKGVELLYHAVTAIDSRGAKG"
     gene            complement(2386..2907)
                     /gene="alaE"
                     /locus_tag="PLM30_04030"
     CDS             complement(2386..2907)
                     /gene="alaE"
                     /locus_tag="PLM30_04030"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HOR54373.1"
                     /translation="MTDGTVRRSERRDRLVMAAADITAMMIFSTALCMMIEIFIARLT
                     FFQSVQARIAAIPVNLITGRPYGWFRDRLFTFLGIDRTMPLKMIGGDTLAFVIFQVPL
                     YVIVLLFAGATWKQIAVSSAFMSLIFSLAGRPYGIFLDLCRNLARRVLMSLRDRDTVS
                     GELLPETGEEETP"
     gene            3126..3593
                     /locus_tag="PLM30_04035"
     CDS             3126..3593
                     /locus_tag="PLM30_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54374.1"
                     /translation="MAKRLFSSWTILFLLLVSFNAAQAQDLSGYWDVKVQGADHFTDR
                     EPSQVPITETETFMQIDRYDGKVVITFGGFGGVHSATLFKGAAGEDSFVATWWYEAYP
                     YETKVLTGLYEPSSGKIRGRMIYPRVSERPGLVSGWVDVEYVATRRIVMAPGR"
     gene            complement(3613..>4103)
                     /locus_tag="PLM30_04040"
     CDS             complement(3613..>4103)
                     /locus_tag="PLM30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR54375.1"
                     /translation="VWDHPEVVVVLSGMNDEAHIDENLGTAGEALPNSLTPGERALVK
                     RAATRYRELMKVGCSGCGYCMPCPAGVNIPACFEVFNKVHLFGDLEGSKDSYVMRMSG
                     VLQRSGSGFASQCVECGKCLGKCPQGLNIPLILKDVAAELEGPDLEERVLEMARKRNI
                     II"
BASE COUNT          720 a         1324 c         1247 g          812 t
ORIGIN      
        1 tcaaggcgag gttcctgggg agcccctccc tgagcgcgcc gtatatcatc gattcgatca
       61 cggtgaagat gtaggccggc cccgaacccg acatggcggt gaggacgtcc aggtactgct
      121 cgtccacctg ctggatctca cccagttcca tcagtatcct ctcgagcagg ggaagcctct
      181 ccgccgggaa atccggctcg gggtagaaga gggtgaaccc cttgtggatc cggacggcga
      241 tattcgtcat cgccctgacg aagtgggcct tcggcgccgc cagcctcagt atgtccgtcg
      301 aaatggctgc tgcaaaggag acgacggtct tgccgctgac ggtctcggcg atctccttga
      361 ggaccgggac cacctgggcc ggcttgacgg acaggatcac caggtcggca tcctcggcgg
      421 ccgccctgtt gtcggaggtc accttgacgc ccttttcgct gagcccgttc agcttgtcga
      481 agctccggcg cgtcgccgtc accgggtatc gcggcacaag cgcctccgag atggctcccc
      541 cgacgatgcc ggcgccgatc acgcatatcc ttgtctcgtc tttctccttc attgatgatc
      601 ccccctggat aatgaaatgc cgccgacccg gaggtcggcg gccctggatg ccttttgcgt
      661 cgaacaggtc actgggtcag gacaagaccg taccttcccg ggagaggctc tttcgggacc
      721 ttggcgggat gcacgggtta ggtcgggtcg tccttctgac ggaaacctgc atgacaacgc
      781 tcctcgcttg aagtcacctg gatcggttga ggaaaaagat acccccagat cccgctttcg
      841 tcaatacccg gcatcgacct tgcttgaaaa ggggaggatc ttgaggttaa cctttactcg
      901 ggaggggtcc gtaatggcaa gatggctcat ggttgccggc gtggtcctgt tactgttggg
      961 ggcggtcctt cacctcgccc cctggctcct gtcatggttc ggcaaactcc ccggggatat
     1021 ccgctacgag acggggcgga cgagggtttt catacctatc acttcgatgg tcgtcgtcag
     1081 cctggtcctt acgctggtga tgaacctgct ctccaggagg taacgggtac tccggcaggc
     1141 cttacccttt cgctccccgg ctgtcgatgg ctgtcaccgc gtggtagagc agttctacgc
     1201 ccttttgaag gtcctcgaag tcggtccatt cctccgggca gtggctcctg ccccccctgc
     1261 tcgggacgaa gatcatcccc gtggggaaaa agggggccat tatcatggcg tcgtgtcccg
     1321 ccccgctgct catggcccgc caggaaaagc ccagtccgtc ggcctggtcc tggagcaccc
     1381 ggcgtatccc ctcatccagt tccaccggcc cgacttcaag aagttgctcc gcccggtagg
     1441 ccgtcccgtc ggcggcggac ctctcgtcca gttccctcct gaccctgtcc ttgaccgtct
     1501 tgatggagag ggcgtcgagg gagcggatgt ccagggtgaa ttcgacctcg ccggggacta
     1561 cgttcgcggc cccgggcctc acgacgagag aacccaccgt cgccacggtg tctccgccct
     1621 gttccgcggc gaaggatggg accctcccga tgacggccgc cgccgtcctg agggcgtcgc
     1681 gcctcatgtc catgggggtc gtcccggcgt ggtcgggcct cccttcgacg gtcaccctgg
     1741 tgaggtcggt gcccacgatg ctcgtgacga tgcccagttc gaagccctcc ctttccagca
     1801 cggggccctg ctcgatgtgc atttccagga aggcccgcac ttcccccggc tttctcctcg
     1861 cctcggcgat cctcgcgggg tcgaacccaa agcgggttaa ggcctcggcc agggtgaccc
     1921 cgtcctcgtc cttgcgctgt tccagttcct cccgggtgac cccccgggtc atggcgcggc
     1981 ttgagaggaa gccggccccg aagcgccccc cttcctcctc gatcatggct atcatctcga
     2041 cggggtgggc cgtacgcacg cccctctcct ccaggcagcg gagcacttcg agggccgcca
     2101 ccaccccggc ctgcccgtcg aagttgcctc cgtgccgcac ggagtcaaag tgggagccta
     2161 tcatgatggg cgggagggaa gggtcgccgc cctcccttct cccgaaaagg cttgcggcgg
     2221 catcttcgta gacatggacc ccaagttctc taaggcgccc ggacaggtag tcccgggccc
     2281 cgcggtcctc gggcgtcagg gaaaagcggg tgagaccctt acccggggtc gcgttgaaga
     2341 gggccaggtt ttccaggtcc ttccggatcc tgtcgaggtc cgccttcacg gggtctcctc
     2401 ctctcccgtc tcgggcagca actcgccgct gacggtatcc ctatcccgta gggacatcag
     2461 gacacggcgg gcgaggttcc tgcaaagatc caggaagatg ccgtaggggc ggccggcaag
     2521 ggaaaatatc agcgacatga aggccgagga aaccgctatc tgcttccagg tcgcccccgc
     2581 gaagaggagg acgatgacat aaaggggcac ctggaatatt acgaaggcca gggtatcgcc
     2641 cccgatcatc ttcaggggca ttgtccggtc tatccccagg aaggtgaaaa gcctgtccct
     2701 gaaccagccg taaggcctcc cggtgatcag gttgaccggt atggcggcga tcctcgcctg
     2761 gacggactgg aaaaaggtga gcctggcgat gaagatctcg atcatcatgc acagggccgt
     2821 ggagaagatc atcatagccg tgatatcggc ggcggccatg acaagcctgt cgcgtctttc
     2881 actccgcctg acggtcccat ccgtcaaggc actccgcccc cttctcgccc gccggtggac
     2941 cggctcctca agactcgccg gccggccgac ccctaatatc taaatactat atgaaacccc
     3001 cgcgaagggc cagcctcctg gtgtcgcggc ggcagggcgc cggggttctg tatcgcgtag
     3061 gcgagcctgg tgctatgata tcagggtaaa tatccccgat acggacagga aaggagctgg
     3121 aggagttggc gaagaggttg ttttcctcct ggaccatcct ttttttactt ctcgtctcct
     3181 tcaatgccgc ccaggcccag gatctttccg gttactggga tgtcaaggtg caaggcgccg
     3241 atcatttcac cgacagggag ccctcccagg tacccatcac tgagacggag actttcatgc
     3301 agatcgaccg ctacgacggg aaggtggtga tcaccttcgg cggtttcgga ggggtccact
     3361 cagccaccct cttcaagggg gcggcggggg aggacagctt cgtggcgacc tggtggtacg
     3421 aggcctaccc ctatgagacc aaggtcctca cggggctgta cgaaccttcc agcggcaaga
     3481 taagggggag gatgatctac cccagggtct ccgaaaggcc ggggcttgta tcgggctggg
     3541 tggacgtcga atacgtggct accaggagga tcgtcatggc gcccggccgt tgagagcggt
     3601 catctccgcc gttcaaatga taatgttcct tttccgtgcc atttcaagga ccctctcctc
     3661 gaggtcgggc ccttccagtt cggcagcgac gtccttcaga ataaggggga tattgaggcc
     3721 ctgggggcat tttcccaggc atttcccgca ctcgacgcac tgggaggcga aaccgctccc
     3781 gctcctttgc aaaacgccgc tcatcctcat cacgtaagag tccttggacc cctcaaggtc
     3841 cccgaaaagg tggaccttgt taaagacctc gaagcaagcg gggatgttca cccccgcagg
     3901 gcagggcatg cagtacccgc atcccgaaca gccgaccttc atcaactccc tgtaccgggt
     3961 ggccgcccgt ttgacgaggg ccctctcccc gggggtcaac gaattgggga gggcctcgcc
     4021 ggctgtcccc aggttctcat ctatgtgagc ttcatcgttc atgcccgaga ggaccacgac
     4081 gacttcagga tgatcccaga ccc
//
LOCUS       DAOSRG010000085         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_19975_length_4048_cov_9.586026, whole genome shotgun sequence.
ACCESSION   DAOSRG010000085 DAOSRG010000000
VERSION     DAOSRG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19975_length_4048_cov_9.586026"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..570)
                     /gene="mraZ"
                     /locus_tag="PLM30_04045"
     CDS             complement(145..570)
                     /gene="mraZ"
                     /locus_tag="PLM30_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOR54376.1"
                     /translation="MLVGTFEHKFDNKGRIVLPSKFRDELGESAVATVGIDRCVSIYS
                     AERWQELLNRFHQMSFSKGKTRDFLRVLLATANEIQFDASGRALIPSNLRQHAKLELE
                     VSVIGVGDHVELWDTTSWNTYLEEIRNVLPDIAEEVEGL"
     gene            complement(755..1176)
                     /gene="rnpB"
                     /locus_tag="PLM30_04050"
     ncRNA           complement(755..1176)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM30_04050"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1332..1958)
                     /locus_tag="PLM30_04055"
     CDS             complement(1332..1958)
                     /locus_tag="PLM30_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOR54377.1"
                     /translation="MAKKINVNPNRMELSRLKARLKVAIRGHKLLKDKQDALIKAFLE
                     KARSLKDLRESVEKELEDCYRSFLLARAQTFPAMLEQALMITSTGLSLKISKKNIMSV
                     IVPIFEVEQGEASLSYGLGTTTGNLDVALEKFSGLLPRLVSLAAEEKALRLMAVEIER
                     TRRRVNALEHVLIPSFQETIRYITMKLDDQERANLSRLMRIKEIVRSH"
     gene            complement(1991..3403)
                     /locus_tag="PLM30_04060"
     CDS             complement(1991..3403)
                     /locus_tag="PLM30_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR54378.1"
                     /translation="MLPKEYRTISDLSGPLLVVENTENVRYDELVEIELANGERRRGR
                     VLEITRENALVQVFEGTSGIDLDTTKVVFMGKVLMLPVSKDILGRIFNGRGEPIDGGA
                     PIIPDKTLDVNGYPMNPYSRDYPSEFIQTGISTIDGMNPMVRGQKLPIFSGSGLPHNR
                     IAAQVARQSTVLGGHEDFAVVFAAMGITFEEAYFFMEDFRKTGAIERTVMFVNLADDP
                     AIERITTPRLALTCAEYLAFERDYQVLVILTDLTNYCEALREISSARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKKGSITQFPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIFPPVDVMPSLSRLKDKGIGAGKTREDHADLMNQLFAAYARGKEAKELATILGE
                     GALSEEDKAFALFSDRFEDRYVRQGEYENRPIEETLELGWDLLTIIPVRELKRVRDAY
                     IEKYLLPRSGAEKEKQAKGA"
     gene            complement(3393..>4048)
                     /locus_tag="PLM30_04065"
     CDS             complement(3393..>4048)
                     /locus_tag="PLM30_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR54379.1"
                     /translation="GSEGREGSITSIGAVSPPGGDLSEPVSQNSLRVTKVFWGLDANL
                     AYQRHFPAINWLLSYSLYAEKLGEYWDNIYDGEWSVLRTEAMSILEEEDRLREIVRLV
                     GIDALSRQERMVLETAKSLREDFLHQNAYHEVDTYASMDKQFKMLRNIIHFHHLCQNA
                     LERGATIQEINTLPVRDLVARMRYEDEKELKRIDELLPVMKEEVDRLLSEGGMGDVA"
BASE COUNT          788 a         1176 c         1049 g         1035 t
ORIGIN      
        1 cgaggacgac ggcttttctc catttcaaca gaaccccttc cgaatacccc ccgagcccga
       61 gagtagcgtc cacgacgaac ctcaggtttt gagccttccc taatattccg cagacttcag
      121 ggagcatcac cgggatatgg gtgttcataa cccctctacc tcttcggcaa tgtcaggcag
      181 gacgttacgg atctcctcga gataagtgtt ccaggaggtc gtatcccata gttccacatg
      241 gtcgccgacg ccgatgacgc tgacttccag ttccagtttg gcgtgctgtc gcaagtttga
      301 ggggataagg gcacggcctg aagcgtcgaa ctggatctcg ttagccgtcg cgaggagtac
      361 cctcagaaaa tccctcgtct ttcccttgga gaaggacatt tggtgaaatc tgttcagtag
      421 ctcctgccat ctttccgcgg agtatatcga tacacaccgg tcgataccca ccgtggcgac
      481 agcgctctct cccaactcat ccctgaattt tgacgggagc actatccttc ccttattatc
      541 aaatttatgt tcgaaggttc ccaccagcat gggatttacc tcccattttt tgccacttcg
      601 ttccactttg taccactcta tcatcttttt tggtttctct atggttcttt cgaccccatt
      661 gaagtcgttt agataaatct caagtatcat ctttctcaaa atacaataaa aaaggagcgc
      721 ttcgtgaatg aagcgctcct ttaggctacc tatagcggga agcagccctt aagccgggtt
      781 ctgtgcgtgg cggtcattta tctggggtcc ggcttgcgcc gggcctcgag cgaccgtacc
      841 cgagggtcag cgggcagcct catcccctcc tattcggtct tgctccgggc ggggtttgcc
      901 tagcctgccg gtcgcccggc agctggtggg cccttacccc accttttcac ccttacccgg
      961 cactcattcg atactttgga gcaaaactgg catcgatccc tccaacgtaa gccgggatta
     1021 tcagtggggc accaaagtgt tgaatgagtg ccgagcggta tgtttctgtg gcactttcct
     1081 tgggctcacg cccactggag tttctccagc gccctgccct gtggagcccg gactttcctc
     1141 cgccccctga ggggcagcga ccgcttggac tccttcccgc aatatattag tttatcatcc
     1201 tttcgataaa aatgggaaaa acagctccct tctcctcaac ctctggcctt tcgggttgac
     1261 gggcctgtaa aagttcaagg ggagccccaa gtggctcccc ttgagaacga tcaatattgg
     1321 gaaggtattt cctagtgaga gcggactatc tccttgatcc tcatgagcct gctgaggttt
     1381 gcccgttcct ggtcatcgag cttcatggtg atgtaccgta tggtctcctg gaaagaaggg
     1441 atgaggacgt gctcgagggc gttaaccctt cttcgggtcc tttcgatctc gacggccata
     1501 agcctcaacg ctttctcttc cgccgccagg ctcaccagtc tgggaagaag cccggagaac
     1561 ttttccaggg ccacatcgag gttccccgtg gtcgtcccca ggccgtaact cagggatgct
     1621 tcaccctgct ccacttcgaa gatagggacg atgacgctca tgatgttctt ttttgatatc
     1681 ttcagggaaa gacccgtact ggtgatcatc aaggcctgct caagcatggc gggaaaggtc
     1741 tgggccctgg caagtagaaa gctgcggtaa caatcttcca gctccttctc caccgattcc
     1801 ctcagatcct taaggctcct ggctttttca aggaaggcct tgatcagggc gtcctgctta
     1861 tcctttagga gtttgtgccc ccttatggct accttaagtc ttgccttcag acgggacagc
     1921 tccattcggt tagggttgac attgattttt ttcgccatat caggctcccc ttaccatttt
     1981 ttgtcttccc tcacgcgccc ttggcctgtt tttctttttc cgccccagat ctcggcagga
     2041 gatatttctc gatgtaggcg tccctgactc gcttcagttc cctgaccggg atgatggtaa
     2101 gcaggtccca cccgagttcg agggtttcct caatgggcct gttctcatat tcaccctgcc
     2161 tgacataacg gtcctcgaaa cgatcggaaa atagggcgaa ggccttgtct tcctcggaaa
     2221 gtgccccttc cccgaggatg gtggccagct ccttggcctc ttttcccctg gcataggcgg
     2281 cgaaaagctg gttcatcagg tcggcgtgat cctccctggt ctttcccgcc ccgataccct
     2341 tgtccttgag cctggaaagg gagggcatga cgtcgacggg gggaaagatg cccttcctgt
     2401 gcagcgttcg actgaggatg atctgtccct cggtaatata acccgtaagg tcgggtatgg
     2461 gatgggtctt atcatcctcg ggcatcgtca atatggggaa ctgtgtgatg gaacctttct
     2521 ttcctttcag ccttcccgcc ctttcgtaca tcgtcgccag gtccgtatat aggtagccgg
     2581 ggtaacctct cctgcctggg acctcctttc gggccgagga gatctccctc agggcctcgc
     2641 agtagtttgt gaggtccgtt aggatgacca ggacctggta atccctttca aaggccaggt
     2701 attcagcgca ggtgagggca agacgggggg tcgttatgcg ttcaatagcg gggtcgtccg
     2761 ccaggttgac gaacattacc gttctttcaa tcgccccggt cttccggaag tcttccatga
     2821 agaagtaggc ttcctcgaag gtgataccca tggccgcgaa cacaacggcg aaatcctcgt
     2881 gaccgccgag tacggtcgat tgcctcgcca cctgagccgc gatcctgtta tggggcaatc
     2941 cgctcccaga aaagatcggg agtttctgac ctctcaccat ggggttcatg ccgtcgatcg
     3001 tggagatgcc tgtctggatg aactccgagg gatagtccct tgaatagggg ttcatgggat
     3061 agccgttgac gtcaagggtc ttgtcgggga tgataggagc ccccccatcg ataggctctc
     3121 ctcgcccgtt gaagatacgt cccaggatgt ccttgctgac gggaagcatc agaaccttgc
     3181 ccataaagac gaccttcgtg gtatccaggt cgatgccgga tgtaccttcg aagacctgta
     3241 ccagggcgtt ttccctcgtt atctccagca ccctgcccct tctccgttcc ccgttggcca
     3301 actcgatctc gaccagctcg tcgtagcgga cattctcggt attctcgacg accagaagag
     3361 gaccggaaag gtcggatatg gtcctgtatt ctttaggcaa catcacccat gcctccttca
     3421 gaaaggagtc ggtcgacttc ctctttcatg accggcagca attcatcgat ccgcttcagt
     3481 tccttttcat cctcgtatct catcctggct accaggtccc tgacaggaag ggtgttgatc
     3541 tcctggatcg tggcccccct ctcaagggcg ttctggcaaa ggtggtggaa gtgaatgata
     3601 ttcctcagca tcttgaactg cttgtccatc gaggcgtaag tatcgacttc gtggtaggca
     3661 ttctggtgca ggaagtcctc cctgagtgac ttggcggtct ccagcaccat cctctcctgc
     3721 ctggacaggg catcgatgcc cacgagcctg acaatctccc ggagcctgtc ctcttcttca
     3781 aggatcgaca tggcttcggt cctcaggacg ctccattcac cgtcatagat gttatcccag
     3841 tattccccga gcttctcggc gtagagggaa taactgagaa gccagttaat ggcggggaaa
     3901 tgcctctggt aggccaggtt ggcatcgaga ccccagaata ccttggtcac cctgagggag
     3961 ttctggctta ccggttcgga caggtcaccc cctggagggg aaacagcccc gatggacgtg
     4021 atggagcctt ccctgccttc ggacccga
//
LOCUS       DAOSRG010000086         3986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_20373_length_3986_cov_12.900534, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000086 DAOSRG010000000
VERSION     DAOSRG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20373_length_3986_cov_12.900534"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PLM30_04070"
     CDS             <1..171
                     /locus_tag="PLM30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOR54380.1"
                     /translation="SCGSRETTLAMVFKLIQSAEKRRKRIKGFDLIGEVIEGVVFKDG
                     VRATDQPDRSAA"
     gene            complement(240..986)
                     /locus_tag="PLM30_04075"
     CDS             complement(240..986)
                     /locus_tag="PLM30_04075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR54381.1"
                     /translation="MDIPRIFNITESVHRIHNPITPEKLATLGAALRLESGARVLDLG
                     SGSGEMLCTWARDHGVIGTGIDMSRLFTDQAKLRAEELGVADRARFIHGDAAGYVADE
                     KVDVAACVGATWIGGGVAGTIELLARSLRTGGIILIGEPYWRQLPPTEDVAKACLADS
                     ISDFLMLPELLASFGRLGYDVVEMVLADQESWDRYEAAKWLTMRRWLEANPDDEFAKE
                     VRDQLTSEPVRYASYSREYLGWGVFALMAR"
     gene            complement(1001..1498)
                     /gene="rhuM"
                     /locus_tag="PLM30_04080"
     CDS             complement(1001..1498)
                     /gene="rhuM"
                     /locus_tag="PLM30_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOR54382.1"
                     /translation="MPGATIRKYRIVQIEGARQVERMIDHYNLEMIIAVGYRVHSNRG
                     TQFRQWATSRLSEYLVKGFTLNDERLKGADSVTDYFDELLARIREIFALARDFRIFAI
                     RTAPAGCCVRSISPSPARVFLARGFIEAGGATSQIKSGAISLPKDVDDSALAAAGCRC
                     CICHK"
     gene            complement(1765..1902)
                     /locus_tag="PLM30_04085"
     CDS             complement(1765..1902)
                     /locus_tag="PLM30_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54383.1"
                     /translation="MEQRDPIETGRPPAGFLVQGVPDAGVDESRIRVGYEIDINRYFY
                     K"
     gene            complement(1988..3397)
                     /locus_tag="PLM30_04090"
     CDS             complement(1988..3397)
                     /locus_tag="PLM30_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR54384.1"
                     /translation="MGDHSLGGRYRGRYGVLGAVMLGSILGPIDASIVNTILPTITSS
                     FGVGISTAQWVTMIYLLTISSLLLFYGRLGDILGYRKVYLFGLLGFVAASGLCSLAST
                     IRWLIAFRAFQGLTAGLTMAVPFAIVTASFPPEERGKALGINAVSISVGLALGPSLGG
                     FITSLASWRLVFLINVPIGLAALAWGWLVIPELKGQPGRIDAGGVVTAFISLFSILFF
                     VRRLQGHGLDRVSGLVLLAAVSAGTAFFRIEKRSPQPLLDLDLFRNRTFTFANVSALL
                     NFMSQYVMVFVTPFFLQGLLGYSPDRVGLVMTAFPLATMAVAPFSGSLSDRVGSRAPA
                     CAGAAFCALALLLMGGLSSSSGAAEVAWRLALFGVGTGLFQSPNHSAVMGNAPRSHLG
                     VASGILGTMRNVGMVFGVAAGGAVLFAFAPPGAFQKALLGPVERAAFSTALGYAYTAG
                     AVMAALSAVTSLVGEKRPG"
     gene            3521..3595
                     /locus_tag="PLM30_04095"
     tRNA            3521..3595
                     /locus_tag="PLM30_04095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3553..3555,aa:Val,seq:tac)
     gene            3627..3703
                     /locus_tag="PLM30_04100"
     tRNA            3627..3703
                     /locus_tag="PLM30_04100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3661..3663,aa:Asp,seq:gtc)
     gene            3720..3795
                     /locus_tag="PLM30_04105"
     tRNA            3720..3795
                     /locus_tag="PLM30_04105"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3753..3755,aa:Phe,seq:gaa)
     gene            3817..3891
                     /locus_tag="PLM30_04110"
     tRNA            3817..3891
                     /locus_tag="PLM30_04110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3849..3851,aa:Gly,seq:gcc)
     gene            3906..3979
                     /locus_tag="PLM30_04115"
     tRNA            3906..3979
                     /locus_tag="PLM30_04115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3938..3940,aa:Cys,seq:gca)
BASE COUNT          843 a         1226 c         1197 g          720 t
ORIGIN      
        1 agctgcggat cgagagaaac gacactggct atggtcttca agctgatcca gtcagctgaa
       61 aagaggcgga agcgtatcaa ggggttcgat cttatcggcg aagtgatcga aggcgtcgtt
      121 ttcaaagacg gtgtaagggc aacagatcag ccagacagga gcgccgcttg agaccccgta
      181 caccagattt gacaataact cccttctcct cccccgttaa ccaaaagcct tcagcgttat
      241 caacgggcca tcagtgcgaa cacgccccac cccaagtatt cacgcgagta agaggcgtag
      301 cgcacgggtt ccgaggtaag ttggtctcga acctcttttg caaactcgtc gtcgggattg
      361 gcttcaagcc atcggcgcat ggtgagccat ttggcagcct catacctgtc ccagctctct
      421 tgatcagcca gaaccatctc aacgacgtca tagccaaggc ggccgaagga cgcgagaagt
      481 tccggaagca tgagaaagtc ggagatcgag tcggcaaggc acgccttcgc aacatcctcc
      541 gtcggcggta gctgccgcca atagggctcg ccgatgagga tgatccctcc ggtgcgcagg
      601 ctccgcgcca ggagctcgat agtgccggct actcccccgc cgatccacgt ggcaccgaca
      661 caggccgcta catcgacctt ttcatcggcg acatagccgg cggcatcgcc atggatgaac
      721 ctggctcgat cggcgacgcc tagttcctcg gcgcggagtt tcgcttgatc agtgaacaac
      781 cgactcatgt cgatgccggt gccgatgacg ccgtggtcgc gcgcccaggt gcacagcatc
      841 tcccccgaac cgctgccgag gtccagcacc cgggctcccg actccaggcg cagcgccgcg
      901 cctagagtgg cgagcttctc gggcgtgatc gggttatgaa tgcggtgaac gctttcggtg
      961 atgttgaata tccgtggtat atccaatgca gaacatctcc ttacttgtgg cagatgcaac
     1021 agcggcatcc cgcagcggca agggccgaat cgtcgacatc cttcggtaag cttatcgcgc
     1081 cactcttgat ttgagaagtc gctccgccgg cttcaataaa tccacgggcc agaaacactc
     1141 gggctgggga tggcgagatc gaccggacac aacacccggc aggggccgta cgtatcgcga
     1201 agatgcggaa gtcgcgggcc agggcgaaga tctcccggat gcgggccagc aattcatcaa
     1261 aataatcggt aacagagtcg gcacctttga gtcgttcgtc gttgagggta aagcctttga
     1321 ccaggtactc gctcagacgt gacgtggccc actggcggaa ctgagtgcct cgattggagt
     1381 gaacgcggta accgactgct atgatcattt caaggttgta gtgatcgatc atgcgctcaa
     1441 cttggcgagc tccctcgatt tgaactattc gatattttcg aatagttgcc ccgggcaata
     1501 actccccatc cctataaatc tctttaacat ggtgatttat gggatttact ccgacatgag
     1561 tcgttgagta agccagacgg ttccaccttc aagcagaaca cggatttgac cgccgtcgcc
     1621 ttccgcctga taaattatga tctcccctcc gggatagggg ggttttctgt tggctcattc
     1681 ggtcacctcc gcgagcaagc tggtgattac tttctcgatc ttctcgaggt cggctttgat
     1741 cttttcaaac ggccttgaca gtatctattt gtagaaatag cggttgatgt caatttcgta
     1801 gccgaccctg atcctagatt cgtcgactcc tgcgtcgggc acaccctgca ccaaaaaacc
     1861 ggcggggggt ctccccgttt cgatcgggtc gcgctgttcc attgatgata catcatagga
     1921 acgccatgaa ggggccgcac ccgaggaaca accgggccgg aagccgcccc atcatcggaa
     1981 gcgagcccta cccgggcctt ttctccccca ccagggacgt gaccgccgac agcgctgcca
     2041 tgaccgcccc ggccgtgtag gcgtacccca gcgcggtgga aaaggccgcc ctttccacgg
     2101 ggccgaggag ggccttttga aaagcccccg gcggggcgaa ggcgaagagc accgcccctc
     2161 ccgccgcgac gccaaagacc atccccacgt tgcgcatcgt gccgaggatc cccgaagcca
     2221 cccccaggtg ggaccttggg gcgttaccca tcaccgcgct gtggttgggt gactggaaaa
     2281 ggccggtgcc gacgccgaaa agggccagcc tccaggccac ctcggccgcc cccgatgagc
     2341 tcgacagtcc ccccatgagg aggagggcca gggcgcaaaa agccgcgccg gcgcaggccg
     2401 gggccctgga gcccacccgg tcggagaggg agccgctgaa gggggccacc gccatggtgg
     2461 ccaacgggaa ggctgtcatc accaacccca ccctgtcggg cgagtacccc agcagcccct
     2521 ggaggaagaa gggcgtgacg aagaccatca cgtactggga catgaagttg agcagggcgc
     2581 tcacgttggc gaaggtgaag gtccggttcc ggaaaaggtc gaggtcgagc agcggctgcg
     2641 gcgacctctt ctcgatccgg aagaaggcgg tccccgccga tacggccgcc agcagcacca
     2701 gccccgagac ccggtccagg ccgtggccct ggaggcggcg gacgaagaaa aggatggaaa
     2761 agagggagat gaacgccgtg accacgcccc ccgcatcgat cctgccgggc tggcccttca
     2821 gctcggggat gacgagccag ccccaggcca gggcggcgag gccgatgggg acgttgatga
     2881 ggaacacgag gcgccagctc gccagggacg tgatgaaccc gcccaaggaa ggccccagcg
     2941 ccaggccgac ggagatgctg acggcgttta tccccagggc cttgccccgc tcctcggggg
     3001 ggaacgaggc cgtcacgatg gcgaagggca ccgccatcgt cagccccgcg gtcagcccct
     3061 ggaaggcgcg gaaggcgatg agccaccgga tcgtagaagc gaggctgcac agccccgagg
     3121 ccgccacgaa acccaggagg ccgaagaggt agaccttccg gtaccccagg atatcgccca
     3181 ggcggccgta aaagagcagg aggctgctga tggtgaggag gtagatcatg gtcacccatt
     3241 gggccgtgga aatgcccaca ccgaaactgc tggtgatcgt gggcaggatc gtgttgacga
     3301 tgctcgcgtc gatgggcccc aggatgctcc ccagcatgac cgcccccagg acaccatagc
     3361 ggccccggta ccgcccaccc aggctgtgat cccccatttt cgatcacccc tccacccttc
     3421 cgttcgctgg tcatcctgcc tctttatagt ctactcccac ccttgttgac agaccatcgg
     3481 ggggtcgata tactaacctt cgcgccactc tggcgtcttg gggcggatag ctcagcggaa
     3541 gagcacctgc cttacaagca gggggtcaca ggttcgaacc ctgtttcgcc caccaggaat
     3601 cttctcaaag gttataatag atggacgggg ttgtagctca gtcggttaga gcgccggcct
     3661 gtcacgccgg aggtcgcgag ttcgagtctc gtcagccccg ccaaattcct tgaaaaaagg
     3721 gcgagatagc tcagtaggta gagcagcgga ctgaaaatcc gcgtgtcccc agttcgattc
     3781 tgggtctcgc caccataaga atccatgaga tgtgaggcgg aagtagctca ggggtagagc
     3841 acaaccttgc caaggttggg gtcgcgggtt caaatcccgt cttccgctcc atttttccct
     3901 ttgcgggcgg catagccaag tggtaaggca gaggactgca aatcctttat ccccggttcg
     3961 aatccgggtg ccgcctccac ttcagc
//
LOCUS       DAOSRG010000087         3949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_20627_length_3949_cov_9.101952, whole genome shotgun sequence.
ACCESSION   DAOSRG010000087 DAOSRG010000000
VERSION     DAOSRG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20627_length_3949_cov_9.101952"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLM30_04120"
     CDS             complement(<1..433)
                     /locus_tag="PLM30_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOR54385.1"
                     /translation="MDGDMDEEETLSLVVGEDLDGERLDVFLTWKLDRTRSQVQALIR
                     DGKVRLEQGQRVRPSMLVRSGMGISVSLEPRPARELIPEPVFFGVVWEDEDILVVDKP
                     SGLIVHPSPGHWKGTLVHGLLDKFPEIAESGAPGRPGIVHRL"
     gene            complement(448..3255)
                     /gene="ileS"
                     /locus_tag="PLM30_04125"
     CDS             complement(448..3255)
                     /gene="ileS"
                     /locus_tag="PLM30_04125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR54386.1"
                     /translation="MSTDYKNTLNLPDTSFPMRANLVRKEPGIIAFWEEKGVYRKLMT
                     EREGAVKFVLHDGPPYANGNIHIGTAFNKILKDFVPKYKWMTGFQSPYVPGWDTHGLP
                     IELRVLKEIGRSKETIDPVELRQQCKSYAEKYIDIQRSEFKRLGVLGDWENPYITFKP
                     EYEAAQIGVFADMVEKGLVYKGRKPVFWCIDCQTALAAAEIEYEDETSPSVYVAYPLI
                     TSGNSAVEEAIRGSEVYVVVWTTTPWTLPASLAVAIHPDFDYAFVPARDGKVFIIAKA
                     LKGKVASVTGLELGEPLLTVKGRDLAKARALHPFYDDREMIFALAEYVGLDQGTGCVH
                     TAPGHGAEDFETGIRYGLDILNPVDDKGYFLPGTKLVGGLSLEEGSKKVLAVLRERGR
                     LLGSGRITHSYPHCWRCKKPVIFRATEQWFVAVNAFKENALDQIDRVRWIPEWGKERI
                     ANMVKERSDWCISRQRIWGVPIPAFYCESCGGLVADPPRIRKVQDLFRREGSNSWWRL
                     SPGQILGELAFCPHCGSRELRKESDIMDVWFDSGCSQAAVLASRKDLKWPADLYLEGS
                     DQHRGYFQTSLLTSVATTGRAPYDMVLTHGFIVDGEGRKMSKSLGNVIAPQEIIEKYG
                     ADILRLWVASTDYRNDVRISEQILSNLVESYRRIRNTARFLLGNLKDFDPGKDRVPFA
                     KMPELERWILSRLQALIRKATRGYDEFEFHVPAYMIHHFCVNELSAIYLDVRKDRLYA
                     EDRAGEARRSCQTALWAILRALTEMLAPVLSFTSEEVWQEMRSLDPSIPESVFLSHWP
                     DVDETLIDEELDSRWERILEQRGALSRALEAARTNGVIGHSLDAQVEILRGETVEDPA
                     RLLPEEEWATLSIVSSLRVVGSFGEVGFSWKDLETGMEFNIRKAPGGKCPRCWRWAPE
                     VPERGVCERCQGVLERMNGSS"
     gene            complement(3360..>3949)
                     /locus_tag="PLM30_04130"
     CDS             complement(3360..>3949)
                     /locus_tag="PLM30_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR54387.1"
                     /translation="GGILIVSLLLTMVACILLGAGLPTTANFIVTSTMAAPALLQLGV
                     APMAAYMFVLYFGIAADLSPPVALAAYAGAGIAGADPMKTGWTAIKLALAGFLVPYIY
                     VFNPMLVLVDFEAFPFIMSIGTALLGVFLLGMGTIGFYKTRMAFWVRALAMAGALGLL
                     VPGIRSDSIGLAVLLFVHFFQTARSKKEALMTKIA"
BASE COUNT          783 a         1263 c         1086 g          817 t
ORIGIN      
        1 ccaggcggtg gactattcca ggccgtccgg gcgctcccga ctccgctatc tcggggaatt
       61 tgtccagcag gccgtggacc agggtaccct tccaatgacc gggggaagga tggactataa
      121 ggcccgaggg tttatccacg acgaggatgt cctcatcctc ccataccacc ccgaaaaaga
      181 ccggttccgg gatgagctcc cgggcgggcc tgggctccaa ggatacgctg atacccatgc
      241 cgctgcggac caacatcgac ggcctgaccc tctggccctg ttcgagtctc accttcccgt
      301 ccctgatgag cgcctggacc tgggacctgg tcctgtcgag cttccatgtc aggaagacat
      361 cgagcctctc gccatccagg tcctcaccta cgaccagcga aagggtctcc tcttcgtcca
      421 tgtccccatc caccccggcg ccttcgctta actcgagccg ttcatccgct ccaggacacc
      481 ctggcaccgc tcgcaaacgc cgcgctccgg tacttccgga gcccatctcc agcacctcgg
      541 gcatttccct cccggtgctt tcctgatatt gaattccatc cccgtctcca ggtccttcca
      601 cgagaagccg acttcaccga aggaccctac gacgcgcaac gaggatacta tcgacagggt
      661 cgcccattcc tcttcgggca gaagcctcgc cggatcctcc acggtttcgc ccctcaggat
      721 ctcgacctga gcgtcgaggg agtgaccgat caccccgttg gtccttgccg cctcgagggc
      781 tctagatagg gccccccttt gttcgaggat cctctcccag cggctgtcaa gctcttcatc
      841 gataagggtc tcgtcaacat cgggccagtg ggaaagaaag acgctctctg gtatggaggg
      901 gtccaggctc ctcatctcct gccatacctc ctccgaggta aagctcagca ccggggccag
      961 catctccgtc aaggcacgaa gtattgccca cagggccgtc tggcagcttc tcctcgcttc
     1021 accggcgcgg tcttccgcgt aaagccggtc tttgcggacg tcgagataga tggcactcaa
     1081 ctcgttcacg cagaaatgat ggatcatgta ggcgggtacg tggaactcga actcatcgta
     1141 gcccctggtc gcctttctta tcaacgcctg caatcttgaa aggatccacc gctccagttc
     1201 cggcatcttc gcgaaaggca ccctatcctt ccccgggtca aaatccttga ggttgcccag
     1261 gaggaaccgg gcggtgttcc tgatcctcct gtaggactcc acgaggttgg ataatatctg
     1321 ttccgagatc ctgacatcgt tcctgtaatc ggtggaggcg acccaaaggc gcaggatatc
     1381 tgcgccgtat ttctcaatga tctcctgggg ggcgatgaca ttccccaggg atttggacat
     1441 ctttctcccc tcaccgtcga cgataaagcc atgggtgagg accatgtcgt agggcgccct
     1501 ccccgtcgtg gccaccgagg tcaggaggga ggtctggaaa tagcccctgt gctggtcgct
     1561 tccctcaaga tagagatctg ctggccactt taggtccttc cgggaagcca gcaccgccgc
     1621 ctggctgcaa cctgaatcaa accagacgtc cattatatcc gactctttcc tgagttcgcg
     1681 gcttccgcag tgcgggcaaa aagccagttc cccgaggatc tgccccggcg agaggcgcca
     1741 ccagctgttg gaaccctccc tgcggaacag gtcctggacc ttcctgatcc tgggagggtc
     1801 ggctaccagg ccgccgcagc tttcgcagta gaaagccggt atcgggaccc cccagatcct
     1861 ctgtcggctt atgcaccagt ccgatcgctc cttgaccatg ttggcgatcc tttccttgcc
     1921 ccactcgggt atccatctga cccggtcgat ctggtccagg gcattctcct tgaaagcgtt
     1981 gaccgctacg aaccactgtt cggtagccct gaaaataacg ggcttcttgc acctccagca
     2041 atggggatag gagtgggtga tcctgccgct cccgagaagc ctaccccttt ctctcaggac
     2101 ggccaggacc tttttggaac cctcttcaag ggaaaggccc cccacgagtt tcgtaccggg
     2161 gaggaaataa cccttgtcat ccacggggtt tagtatatcc aggccgtacc gtatacccgt
     2221 ctcgaagtct tcggcaccat ggcccggcgc tgtatgtaca cagccggtcc cctggtcgag
     2281 gccgacgtat tcggctaggg cgaagatcat ctccctgtcg tcgtagaaag gatgcagggc
     2341 gcgggccttg gccaggtccc gcccctttac ggtgaggaga ggttccccga gctcgaggcc
     2401 cgtcacggac gcgaccttcc ccttgagggc cttggctatg atgaagacct tgccgtcccg
     2461 ggcggggacg aaggcgtagt cgaaatcagg gtggatcgcc acggcaaggc ttgcggggag
     2521 cgtccaggga gtggtggtcc agacaacgac gtagacttca ctcccgcgga tagcctcctc
     2581 caccgcactg ttgcccgagg tgatcaaggg gtaggccacg tagaccgagg gcgaggtttc
     2641 gtcctcgtac tctatctccg cagcggcgag ggccgtttgg caatctatgc accagaacac
     2701 cggcttgcgc cccttgtaca cgagcccctt ttccaccata tcggcgaaga cgcctatctg
     2761 ggcggcctcg tactccggct tgaaggttat gtaggggttt tcccagtctc caagtactcc
     2821 aagccttttg aactccgacc tctggatatc gatatacttc tcggcgtaac tcttacattg
     2881 ctgccttaat tccacggggt cgatggtctc cttcgatcgc ccgatctcct tgaggaccct
     2941 gagttctatg ggcaggccat gggtgtccca acctgggacg taaggagact ggaaacccgt
     3001 catccacttg tatttcggga cgaagtcctt aaggatcttg ttaaaggccg tcccgatatg
     3061 gatgttcccg ttggcatagg ggggaccatc gtgaaggaca aatttgaccg ccccctccct
     3121 ctcggtcatc agtttacggt aaaccccctt ctcctcccag aaagcaatga tcccgggttc
     3181 cttcctgacc aggttggccc gcatggggaa agaagtatcg ggaaggttca aggtattctt
     3241 ataatcagtt gacaagggct ggacgcctcc ttctttattc aaaaaggccg ccagttcgag
     3301 ccggcggcct ccccgacgat cgggatactc ggcccaggat caccggtgcc atgccggccc
     3361 taggcaattt tggtcatcaa cgcctctttc tttgaccggg ccgtctggaa gaaatgcacg
     3421 aaaagcagca cggccagtcc tatggagtcc gatcttatcc ccggcaccaa gagaccaagg
     3481 gcccctgcca tggccagggc tctgacccaa aaggccatcc tggtcttgta aaaaccgatg
     3541 gtgcccatgc caaggaggaa cactcccagc aacgccgtgc ctatggacat gataaaagga
     3601 aacgcctcaa agtccacaag gacgagcatg gggttgaaga catagatgta aggcaccaga
     3661 aaacccgcca gggccagttt tatggccgtc cagcccgtct tcatcgggtc cgcccccgcg
     3721 atacccgccc cggcataggc ggccaaagct actggcgggc tcaggtccgc ggcgatcccg
     3781 aagtaaagaa cgaacatgta ggccgccatt ggggctacgc ccagctgcag cagggccggc
     3841 gcggccatcg tacttgtgac gatgaagttg gccgtcgtcg ggagccctgc cccgagcagg
     3901 atacaggcca ccatggtcaa cagcagcgag acgatcagga tccctccgg
//
LOCUS       DAOSRG010000088         3939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_20702_length_3939_cov_7.929454, whole genome shotgun sequence.
ACCESSION   DAOSRG010000088 DAOSRG010000000
VERSION     DAOSRG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20702_length_3939_cov_7.929454"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLM30_04135"
     CDS             complement(<1..362)
                     /locus_tag="PLM30_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOR54388.1"
                     /translation="MADKTLKWEVVELGIPEGCNIILGQSHFIKTVEDLYEVLVTSSP
                     ALEFGLAFCEASGPCLVRKDGNADDLVEAAVENALKLSAGHAFVILMRNGFPISVLNA
                     VKQVQEVCRVFAATANPL"
     gene            complement(352..1251)
                     /locus_tag="PLM30_04140"
     CDS             complement(352..1251)
                     /locus_tag="PLM30_04140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR54389.1"
                     /translation="MRKLLVTDIDGTLAHRDHVCDEVVQTCRRLKDDGWEIIIATGRI
                     LASARRLMRAVGALPQAIVYDGARIMTDGTGEELWGATIPPETVENILGMIWEAAPGI
                     QVFGDEEVYCRPGDLLARRYFSALGVPVMDDLIRPRPIPGIFRVIVHGDPEEVEGIGL
                     RVQGAFGGAARAVFAGDGFLDILGPGISKGAAMRKLLGFLLEKGVPALVAAAGDHLND
                     LEMLEQADIAITMEDAREPLLDVADIILPPASEKGFSKILEPLGSLSGSLEAKIGEDR
                     SRGGSTPCSVSGGRAGGGMDNGG"
     gene            1411..2568
                     /locus_tag="PLM30_04145"
     CDS             1411..2568
                     /locus_tag="PLM30_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54390.1"
                     /translation="MRKILVLALLGVFAVMGLAAAAAAEDVIKIGHLAALTGDWAAYG
                     QTEVNSAKLAVEEINAKGGVLGKKLQLVPYDFRTRPEDAVNAFRRMAENDKVVAVIGA
                     NGSGINIATAPLADKYKVPQIGTVSTNLLVTVDEKGVLHPYMFRICFTDPYQGQLIAA
                     FSAHELKKMTAAVLWDVGSDYAQGLREFFMKYYDQYGGKVVADLGFRAGTDVDFRAQL
                     TTIRDSKAEVLVLPNMGKEMALIMKQARELGMKDIIFVGGDGYGEFMWEIAGDAMEGS
                     YWINHVAPEDPAMADFFKDYLKTYNDECKEFTNGVLAYDSIYWLADAITRAGKVDSTA
                     IKDALENTVNVKLHHATMSIDPATHNPMNKTGVVLIAKDGRGQFFTKIQPE"
     gene            2665..3546
                     /locus_tag="PLM30_04150"
     CDS             2665..3546
                     /locus_tag="PLM30_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54391.1"
                     /translation="METLLQQVINGFSLGSVYALIAVGYSLVYSILLFSNFAHGGFLV
                     IGGYICYYTLAAVGNNIWVASLLAFLGAGVSAVLVERGAYKPIRERTNVTLYLLIASM
                     GMSIVIENIFVVTVGGRFRALPPVIPMTPVSVFGLATTSAFDILSLVMAMIFLAGLQV
                     FLSTTKWGLAIRAASYDLKTAGLMGVNVNKLISIVFFVAGLLAAVGGIFLSVRYTLYP
                     QLGLITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDIVVFGMLVLT
                     LLWRPTGLFGKHISEKV"
     gene            3564..>3939
                     /locus_tag="PLM30_04155"
     CDS             3564..>3939
                     /locus_tag="PLM30_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54392.1"
                     /translation="MSGYIEGIIILICVNSIAAMGVSLLTGFTGIFTLGHAGYMAIGA
                     YTAAILSVRHDVPWFAAVIAGGILAMVLAYLIGVPTLKLMGDYFAIASIGLGETIRLI
                     LENWQSFTRGARGFPGVTNFTTL"
BASE COUNT          739 a         1292 c         1167 g          741 t
ORIGIN      
        1 tgcagcgggt tcgccgtggc cgcgaatacc cggcatacct cctggacctg cttgacggcg
       61 ttgaggacgc tgatggggaa gccgttcctc atcaggatga cgaaagcgtg ccccgccgac
      121 aacttcagag cgttctccac cgcggcctcc acgaggtcgt cggcgttgcc gtccttccgc
      181 accaggcagg gacccgaggc ctcgcagaag gccagcccga actcaagggc cggcgacgaa
      241 gtgaccagca cctcgtagag gtcctccacg gtcttgatga agtgactctg gcccaggatg
      301 atgttgcagc cttcgggaat gccgagttcg accacttccc acttcagggt cttatccgcc
      361 attgtccata cctcctccgg cccggccccc gctgacggaa cagggggtgg atcccccgcg
      421 ggagcggtcc tctcctattt ttgcttccag ggaacccgac aacgagccga ggggttccag
      481 gatcttcgaa aaacccttct ccgacgcggg gggcaggatg atatcggcca catccagcag
      541 gggttccctg gcgtcctcca tggtgatggc tatatcggcc tgttccagca tttcgaggtc
      601 gttcaggtgg tccccggcgg cagccaccag cgccggcacg cccttttcca gcaggaatcc
      661 gagcagcttc ctcatcgcgg cgcccttgga gatgccggga cccaggatgt cgaggaaacc
      721 gtcgccggcg aagacagccc gcgccgcgcc tccaaaggcg ccctggacgc ggaggccgat
      781 gccctcgacc tcttccgggt ccccatggac gatgaccctg aagatccccg gtatgggcct
      841 cggtcggatg aggtcgtcca tcacggggac tcccagggcg ctgaaatacc gccgcgccag
      901 gaggtcgccg ggccggcagt agacctcctc gtcgccgaag acctggatcc ccggagccgc
      961 ttcccagatc atccccagga tattctccac ggtctccggg gggatggtcg ccccccataa
     1021 ctcctcgccg gtgccgtcgg tcatgatcct ggcgccgtcg tagacgatgg cctggggcag
     1081 ggcccccacg gccctcatga ggcggcgggc actggcaagg atgcgccccg tggctatgat
     1141 gatctcccac ccgtcatcct tcagccgccg gcaggtctgg accacctcgt cacagacatg
     1201 gtcccgatgg gccagggtcc cgtcgatatc agtcaccagc aattttctca tggtcccccc
     1261 gatccccccg cccctggagc cgcggcaagg cgatattaac ggtaaacgcg ggaaaaatga
     1321 acgagaaaaa gcaacttgac aaccgcagaa cagcacgaca attatggtat catgtaaatg
     1381 tttccataac cttctcaggg ggtgtaagct ttgaggaaga ttcttgtgct tgccttgctc
     1441 ggtgttttcg cggttatggg cttagcggcg gcagctgcgg ccgaggatgt gatcaagatc
     1501 ggccacctcg ccgccctgac gggcgactgg gccgcctacg gccagaccga ggtcaactcg
     1561 gccaagctgg ccgtggaaga gatcaacgcc aaaggcgggg tcctgggcaa aaagctccag
     1621 cttgtcccct atgacttccg cacccgcccc gaggacgcgg tgaacgcctt ccggcggatg
     1681 gccgagaacg acaaggtagt ggcggttatc ggcgccaacg ggagcggcat caacatcgcc
     1741 accgccccgc tggccgacaa gtacaaggtc ccccagatcg ggacggtgtc gacgaacctc
     1801 ctcgtgacgg tcgacgagaa gggcgtcctc cacccctaca tgttccgcat ctgcttcacc
     1861 gacccgtacc agggccaact gatagccgct ttcagcgccc atgagctgaa gaagatgacg
     1921 gcggccgtcc tctgggacgt gggcagcgac tacgcccagg gcctcaggga gttcttcatg
     1981 aagtactatg accagtacgg cggcaaggtc gtagccgacc tgggcttccg agccggcacc
     2041 gacgtggact tcagggccca gcttaccact atccgtgaca gcaaggccga agtccttgtc
     2101 ctgcccaaca tgggcaagga gatggccctc atcatgaagc aggcccggga actcggcatg
     2161 aaggacatca tcttcgtcgg tggcgacggt tacggcgagt tcatgtggga gatcgccggc
     2221 gacgccatgg agggcagcta ctggatcaac cacgtcgccc ccgaggaccc cgccatggcc
     2281 gacttcttca aggactacct gaagacctac aacgacgagt gcaaggagtt caccaacggg
     2341 gtgctggcct acgactcgat ctactggctc gctgacgcca tcaccagggc cggcaaggta
     2401 gacagcaccg ccatcaagga cgccctggag aacaccgtga acgtgaagct tcaccacgcc
     2461 accatgtcca tcgacccggc gacccacaac ccgatgaaca agaccggcgt cgtcctgatc
     2521 gccaaggacg ggagaggcca gttcttcacc aagatccagc ctgaatagcg tcacctctct
     2581 tttctcgcag tcacccgcaa cggggcgggc gcgaggcttc gcgtccgccc cgcatttgca
     2641 ggacacaagg acggtgaacc cggtttggaa acacttctcc agcaggttat caacggtttt
     2701 tccctcgggt cggtctatgc cctcatcgct gtcggctatt cactggtcta ttccatcctg
     2761 ctcttttcca acttcgccca cggcggtttc ctcgtcatag ggggctacat ctgctactac
     2821 accctcgcgg ccgtgggcaa caacatctgg gtcgcgagcc ttctggcctt cctcggcgcg
     2881 ggagtttcgg cggtcctggt agagcgaggg gcctacaagc ccatccggga aaggaccaac
     2941 gtgaccctct acctcctcat agcctccatg ggcatgagca tcgtgatcga gaacatattc
     3001 gtggtcaccg tgggaggccg tttcagggcc ctccccccgg tcatcccgat gaccccggtg
     3061 agcgttttcg gacttgccac tacgagcgct tttgacatcc tctccctggt catggccatg
     3121 atcttcctcg caggcctgca ggtcttcctc tcgaccacga aatggggcct cgccataagg
     3181 gccgcatcct acgacctcaa aacggcgggt ctcatgggag tcaacgtgaa caaactgata
     3241 tccattgtct tttttgttgc gggcctgctc gcggccgtgg gcgggatctt cctttcggtc
     3301 cgttataccc tttaccccca gttgggcctg ataacgatca aggctttcgt cgccgccgtg
     3361 atcggcgggc tggggagcct gccgggggcc gtcgtgggga gcctgatcct ggggctagcg
     3421 gagatgctca ccgccgggtt catatccagc cagatgaggg acatcgtcgt cttcggcatg
     3481 ctggtcctga cgctcctctg gcgcccgacg gggctgttcg gcaagcacat ctccgagaag
     3541 gtctaggggg gaggcccgtc atgatctcag gttatatcga aggcatcata atcctgatct
     3601 gcgtgaactc catcgcggcc atgggcgtga gcctcctcac ggggttcacg ggtattttca
     3661 cccttggcca cgcgggctac atggccatcg gggcctacac ggcggccata ctatccgtcc
     3721 gacacgacgt cccctggttc gcggccgtca tagccggcgg gatcctggcc atggtcctgg
     3781 cctatctcat cggggtcccc acgctgaaac tgatggggga ctacttcgcc attgcctcca
     3841 tcgggctcgg cgagacgata agactcatac tggagaactg gcaatccttc accagggggg
     3901 ccagggggtt ccccggggtg acgaacttca ccaccctcc
//
LOCUS       DAOSRG010000089         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_20779_length_3930_cov_12.338065, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000089 DAOSRG010000000
VERSION     DAOSRG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20779_length_3930_cov_12.338065"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLM30_04160"
     CDS             complement(<1..683)
                     /locus_tag="PLM30_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOR54393.1"
                     /translation="MDRASRVVIALGGNALQEAGAPPTAEAQLEVVRKTAGFLAEISR
                     KGYEMAIVHGNGPQVGRIVLSQEIASRENPQTPAMPFDVCGAMSQGYIGYHIQQALSE
                     ALRDRGRDIPVVTLVTQVVVDPGDPAFSNPTKPIGPFYTEGEAKAIARERGFTMKEDA
                     GRGWRRVVPSPDPKRIVELSAVKRLWDTSIVITAGGGGIPVVEKGDGSLAGVAAVIDK
                     DLAAGRLAQ"
     gene            complement(776..1486)
                     /locus_tag="PLM30_04165"
     CDS             complement(776..1486)
                     /locus_tag="PLM30_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOR54394.1"
                     /translation="MRFCIAPEVFEKWPEYRRCTVVGRGLENAEDDPALLALLREAEA
                     GVRGDPALEDYKEYPLIASWRSVFREMGLNPNKFPPSIANLIRRTRSGKDLPFINRIV
                     AIMNIISLKHRIPCGGDDLSGLAGDLRLGPARGDEPFAQLGDPGTVENPDPGEIILHD
                     TAKGTVFCRGWCWKNGDPSKITALTSHVALHLDFLPPLEVAARERAVEEIVGLLEGRC
                     GGSVAARLIEPGNPCCEI"
     gene            1568..>2074
                     /locus_tag="PLM30_04170"
     CDS             1568..>2074
                     /locus_tag="PLM30_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR54395.1"
                     /translation="MKKEQTAGGRKALFFFCVGEGYDHVSRQAFEKVLELYRPDETEI
                     SVDGYPVMEYVDGRGDRFFFMRQETLVSYDFRRYLPVLKGHFLDVDLAAEVNWHEGAN
                     APDRVLTVHTVGDVNAGVFPPAEPAYVRNFLRALEDERVRAGVDDFSVVTEATHWTGT
                     FKGQDPRLM"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2175..2673
                     /locus_tag="PLM30_04175"
     CDS             <2175..2673
                     /locus_tag="PLM30_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR54396.1"
                     /translation="TEATHWTGTFKGQDPRLMGGFPVPMLDIEVGSTPASWEDPRAVG
                     VMARSLVKPFSESQRLYRVLCVGGVHFERSFSEAALGDFPFGVSHILPNQWIVTGDYA
                     SEGGYEKLRSAASSIRGGIDAVVYHEGIKGAFRDQCRRLAGELGVPVLKHKALRRPEA
                     LGFTP"
     gene            complement(2683..3378)
                     /locus_tag="PLM30_04180"
     CDS             complement(2683..3378)
                     /locus_tag="PLM30_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54397.1"
                     /translation="MDDWTLEVLAGRMSGDTSAPHHIAAVLRDAIYRGVLEEGGPLHQ
                     ARIAQSLGVSPIPLREALRLLETEGLVEFRGYRGAFVTELSLEEARELYEMVAALETS
                     LMRTAFPGITRKIIQDAGEVLDRMEKEEDCIRWRDQNFLFHTLFYEPADRPLTLDMVS
                     RLRQKTDRTIRTHLGSMRDESQRQHREILAAVTAGDLEGSVAALAHHLAYTSSDLQSW
                     MRDEQSRKKRPEL"
     gene            3561..>3930
                     /locus_tag="PLM30_04185"
     CDS             3561..>3930
                     /locus_tag="PLM30_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54398.1"
                     /translation="MQFLTEELEAMKRDGLYGMIRTIESPQGAWVRIAGKDHLNLCSN
                     NYLGLCNDPRVVDKVKEYVDKWGVGPGAVRTIAGTMTPHIELERRLAKFKGAEAAIVL
                     QSGFCANLAVIPTLVPAAEDT"
BASE COUNT          613 a         1284 c         1250 g          683 t
ORIGIN      
        1 tcctgggcca gccgccccgc cgcgaggtcc ttgtcgatca cggcggccac gcccgcgagt
       61 gagccgtcac ccttctccac gacggggatg ccgccgccac cggcggtgat cacaatggag
      121 gtatcccaga ggcgtttcac ggcggagagc tccacgatcc tcttggggtc cggcgacggg
      181 acgaccctgc gccagccgcg gccggcgtcc tccttcatgg tgaaacccct ctcccgggcg
      241 atcgccttgg cctcgccctc ggtgtaaaag gggccgatgg gcttcgtggg gttgctgaag
      301 gcggggtccc cggggtcgac gacgacctgg gttacgaggg tgaccaccgg tatgtcgcgg
      361 cccctatccc tcagggcctc gctgagggct tgctggatgt ggtagccgat gtaaccctgg
      421 ctcatggccc cgcagacgtc gaagggcatg gcgggggtct gggggttttc cctggaggcg
      481 atctcctggg agagtacgat cctccccacc tggggcccgt tgccgtggac aatggccatc
      541 tcgtagccct tgcggcttat ctccgccagg aacccggcgg tcttccttac gacctcgagc
      601 tgcgcttcgg ccgtaggcgg cgcgcccgcc tcctggaggg cgttcccccc cagggctatc
      661 acgaccctgg atgcacgatc catagacctc tctcctcacc gtgaaagcga tattttcggg
      721 aaggatttta agcccttccc cttgagtggt tcaacccctc cgttccgaag ggcggtcaga
      781 tctcgcagca ggggttcccc ggctctatca gccgggccgc gacgctcccg ccgcagcggc
      841 cctccaggag gcccacgatc tcctcgacgg ccctttcgcg ggccgcgacc tccaggggcg
      901 gcaggaagtc caggtgcagc gccacgtggc tggtcagggc cgtgatcttg ctggggtcgc
      961 cgttcttcca gcaccacccc cggcagaaga ccgtcccctt cgccgtgtcg tggaggatga
     1021 tctcccccgg gtcggggttc tccaccgtcc cggggtcgcc cagctgggcg aagggttcgt
     1081 cgcccctcgc cggtcccagc cggaggtcac ccgccagccc cgacaggtcg tcgccgccgc
     1141 aggggatccg gtgcttcagg cttatgatgt tcatgatcgc cacgatcctg tttatgaagg
     1201 gcaggtcctt gccggagcgg gtccgcctga tgaggttggc gatggacggc gggaacttgt
     1261 tggggttgag ccccatctcc ctgaagacgg agcgccagga cgcaatcagg gggtattcct
     1321 tgtagtcctc tagggcgggg tcccccctga cgcccgcctc ggcctccctg agcagggcca
     1381 gcagggcagg gtcgtcctcg gcgttctcca gtccccggcc gaccacggtg caacggcggt
     1441 actccggcca tttctcgaag acttcgggtg ctatgcagaa tcgcaagggg tgcatctcct
     1501 ttcatcgaca gccgcggctt ctttcacgcc ctgtataata acagcacttc cagggggagg
     1561 cgaagaaatg aaaaaggaac agaccgccgg ggggagaaag gccctcttct ttttctgcgt
     1621 cggcgagggt tacgaccacg tctcgaggca ggccttcgag aaggtcctgg aactttaccg
     1681 ccccgatgag acggagatct ccgtcgacgg gtaccccgtc atggagtacg tcgatggccg
     1741 gggggaccgt ttcttcttta tgcgccagga gaccctcgtc agttacgact tccgtcgtta
     1801 cctgcccgtc ctcaaggggc acttcctcga cgtcgacctc gcggccgagg tgaactggca
     1861 cgagggggcc aacgcccccg acagggtcct gacggtccac accgtgggcg atgtgaacgc
     1921 cggcgtcttc ccccccgccg agccggccta cgtgaggaac ttcctccgcg ccctggagga
     1981 cgagagggtc cgggcggggg ttgatgactt cagcgtggtg accgaggcca cccactggac
     2041 ggggaccttc aaggggcagg acccccggct gatgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnngaccga ggccacccac tggacgggga ccttcaaggg gcaggacccc
     2221 cggctgatgg gcgggttccc cgtccccatg ctcgacatcg aagtgggcag cacccccgcc
     2281 agttgggagg acccgcgggc ggtgggggtg atggccagga gcctggtgaa gcccttttcg
     2341 gaatcccaac gcctctaccg ggtcctatgc gtgggcgggg tccacttcga aaggtccttc
     2401 tccgaggcgg ccctgggcga cttccccttc ggcgtatccc acatactccc taaccagtgg
     2461 atcgtcaccg gggactacgc ctcggaaggg ggttacgaaa aactgcgctc cgccgcgtcg
     2521 agcatcaggg gaggcatcga cgccgtggtc taccacgagg ggataaaggg cgctttcagg
     2581 gaccagtgcc ggaggctcgc cggcgaactg ggcgttcccg tcctgaagca caaggccctg
     2641 cgccggcccg aggcgctcgg cttcacccct tgagcggggc cttcatagct ccggcctttt
     2701 cttcctcgac tgttcgtccc tcatccagga ctgcaggtcg ctggaggtgt aggccaggtg
     2761 gtgggccagg gcggccacgg acccctcgag gtcgccggcc gtcaccgccg ccaggatctc
     2821 ccggtgctgc cgctgggatt catccctcat cgagcccagg tgggtcctga tcgtgcggtc
     2881 cgtcttctgc ctgagcctcg agaccatgtc gagggtcagc ggtcggtcgg ccggctcgta
     2941 gaaaagggtg tggaagagga aattctggtc ccgccagcgg atgcagtcct cttccttttc
     3001 catccggtcg aggacctcgc cggcgtcctg gatgatcttg cgggtgatcc ccgggaaggc
     3061 cgtcctcatc aggctggtct ccagggcggc caccatctcg tagagctccc gggcctcctc
     3121 cagggagagc tccgtcacga aggcaccccg gtacccccgg aactccacga gcccctccgt
     3181 ttcgagcagc ctcagggcct ccctcagcgg gatggggctc acgccgaggc tctgggcgat
     3241 cctggcctgg tggagcggcc ccccctcctc gagcacgccc cggtagatgg cgtcccgcag
     3301 caccgccgct atgtggtggg gcgcgctggt gtcgccgctc atccttccgg ccagtacctc
     3361 cagggtccag tcatccatcg gtgaagacct ccccgctttc cccctcgggg ggggacctgg
     3421 gaatattata ttatcccgac cgccgattat acgaaccgcc cttcaaacta tacctcgttg
     3481 acacttcccc ctggtgaaaa gcatactggt tccggcaagg tccaaatacg gaagcgggag
     3541 tgatagccgt ggcggtaacg atgcagttcc tcaccgaaga gctggaggcc atgaagcgcg
     3601 acggcctcta cgggatgatc cgcactatag aaagccccca gggggcgtgg gtccggatag
     3661 cgggcaagga ccacctgaac ctctgctcca acaactacct ggggctctgc aacgacccga
     3721 gggtggtgga caaggtcaag gagtacgtgg acaagtgggg cgtggggccc ggggccgtga
     3781 ggaccatcgc cgggaccatg acgccccaca tagaactgga gagacgcctg gccaagttca
     3841 agggggccga ggcggccatc gtgctccagt cggggttctg cgccaacctg gccgtcatac
     3901 ccaccctcgt ccccgcggcg gaggacacca
//
LOCUS       DAOSRG010000090         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_20889_length_3914_cov_5.006219, whole genome shotgun sequence.
ACCESSION   DAOSRG010000090 DAOSRG010000000
VERSION     DAOSRG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20889_length_3914_cov_5.006219"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLM30_04190"
     CDS             complement(<1..868)
                     /locus_tag="PLM30_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOR54399.1"
                     /translation="MKAKAREIADSPLEWLDGTGEYSDIILSSRVRLARNLKGHAFPW
                     RAGKEELESCAEKVGAIIRREDSLRSFGLFEVDALEPLAKGVLIEKHLISPTLAKGGP
                     GRGAAVDSRGIVSIMINEEDHIRIQAILGGLRIHEAYRIARQMDELLEALDYAFDSRS
                     GYLTSCPTNVGTGLRASVMIHLPALTHLGRLGKLAVGCGKVGLTVRGMFGEGSESPGA
                     LYQISNQVTLGQTEDELVEKVSGAALGMVRLEQAVRSKIFSERGPKLEDKVWRAWGLL
                     GFSRVMEAREALE"
     gene            complement(852..1388)
                     /locus_tag="PLM30_04195"
     CDS             complement(852..1388)
                     /locus_tag="PLM30_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOR54400.1"
                     /translation="MKCERCGLREAEVHIRHQKGKEKVDFHLCRQCAEQMAKDGLLPD
                     IPLEMPLDCFPWGLFPWQGAATPGLETDSPPADQRACSHCGLDHSSFRKTGKFGCPDC
                     YEVFRDDLGPLFRKIHGSDIHRGARPRNACGDEVSPEELGSLRKELREAVEKEEYERA
                     AVIRDRIREIEECHEGEG"
     gene            complement(1400..1879)
                     /locus_tag="PLM30_04200"
     CDS             complement(1400..1879)
                     /locus_tag="PLM30_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOR54401.1"
                     /translation="MASLTRIIERHIVELLERGSEESVFLSRKDLAERFGCVPSQINY
                     VIRSRFTPERGYIVESQRGGHGYIRVIRLCLDMPEERANHIEELVGDGVNEQDIRRIL
                     ANLQHRELLTARERLLVEVATRFQEEIGRDLLDLSAYKRESLHAELLKRLLRSLALG"
     gene            complement(1970..2557)
                     /gene="plsY"
                     /locus_tag="PLM30_04205"
     CDS             complement(1970..2557)
                     /gene="plsY"
                     /locus_tag="PLM30_04205"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOR54402.1"
                     /translation="MTVAAWVIAGYLFGSIPSGYLAVRMLLGKDIRTLGSGNIGATNV
                     TRFAGRKWGVAVALFDMAKGGLVVLAARLFGVQEPLVLALAGFAGVLGHNFPLWLNFK
                     GGKGVATSFGVIFFWNPVPALIGGVAWYLVMKLSKYVSVGSMVSLATVPVWFVLFKGP
                     PAYIWTSAAMAALAVARHHTNILRLVKREEEPIGS"
     gene            complement(2557..>3914)
                     /locus_tag="PLM30_04210"
     CDS             complement(2557..>3914)
                     /locus_tag="PLM30_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR54403.1"
                     /translation="RSPTGQAAVKSHTAALTGEDEAWRTIFRQYGVLEIEDSDDIADL
                     GKIFGPVKTPRGKRVGVITTSGGAGIIMADRAYDLGLKVEPLPRETREALERFVPPYG
                     STRNPVDLTAQVINDPGSFKSSLETVLDCPEIDMASVVISMITGDSGWSVTRDIIEVS
                     RKASKPMACCWLINNEQGGPFLEELRSEKVPLFASPRRCAWALARLSEWVTRPALPAR
                     APSDPGDPFLPRLPEELTEYDAKRLLARWGVPVTREILCSSLEDVLSAASEIGYPVAL
                     KVMSPDIIHKTEAGAVALRLKDEEELRNAFGRLLERSLKAMPGARVRGMLVQEMVTGG
                     VECMIGVKRDPVFGPFVAVGLGGIYVEILKDLSLRHAPVDVEEARRMIASLKGFPLLS
                     GARGSVSKDTEALAGMVSRVSLMALAEESLAELDINPVFVLDEGGGAVAVDAAAIRGP
                     R"
BASE COUNT          719 a         1275 c         1124 g          796 t
ORIGIN      
        1 gttccagggc ttccctggct tccatgaccc ttgaaaaacc gagcaggccc caggctctcc
       61 agaccttgtc ctcgagtttc ggccctcttt cggagaagat cttgctcctg accgcctgct
      121 ccagcctgac catcccgagg gccgcgccgg agaccttttc gaccagttca tcctcggtct
      181 ggccaagggt aacctggttg gagatctggt agagcgcacc gggtgactcg ctcccctctc
      241 cgaacattcc tctgacggtc aggccgacct ttccgcaacc gacagccagc ttgcccagcc
      301 tgccgagatg ggtgagggcg ggcaagtgga tcatcaccga ggcccggagt cccgtcccca
      361 cattcgtggg acaggacgtc aggtaaccgc ttctgctgtc gaaggcataa tccagcgcct
      421 cgaggagttc atccatttgc cgggcgatcc tgtaggcttc atggattctc aaccctccga
      481 gtatggcctg gatccgaata tgatcctcct cgtttatcat gatcgagacg atccctcggc
      541 tgtccacggc ggcgccccga ccaggccccc ccttcgcaag ggtggggctg atgaggtgct
      601 tctctatgag gacccccttc gcaaggggct ccagggcatc gacctcgaaa agtccgaagg
      661 accgcaggga gtcttccctg cggatgattg caccgacctt ttcggcacaa ctttcgagtt
      721 cctctttacc ggccctccag gggaaagcat gccccttcag gttcctggcg agccttaccc
      781 ggcttgaaag gatgatatcc gagtactcgc cggtcccatc aagccactcg agagggctgt
      841 cggcgatctc cctagccttc gccttcatgg cactcctcga tctcccttat cctgtccctg
      901 atgaccgcgg ccctttcgta ttcttccttc tcgacggcct cccggagttc tttcctcagg
      961 gaccccagct cttccgggga tacttcgtcc ccgcaggcat tcctgggcct tgccccccgg
     1021 tgtatgtccg acccgtggat cttcctgaag agggggccga ggtcatcccg gaaaacctcg
     1081 tagcagtcgg ggcacccgaa cttgccggtc ttcctgaagg aagaatggtc gagaccgcag
     1141 tggctgcagg cccgttgatc ggcgggcggt gagtccgttt cgagaccggg ggtcgcggcg
     1201 ccttgccacg ggaaaagccc ccaggggaaa cagtccaggg gcatctccag cgggatgtcc
     1261 ggcagcaggc catccttggc catctgctcg gcgcactggc ggcagaggtg aaagtcgact
     1321 ttctccttac ccttctggtg cctgatgtga acttccgctt cccttaaccc gcacctttcg
     1381 catttcatgg cctcacgacc taaccgagag ccaggctgcg caaaagccgc ttgagaagct
     1441 ccgcgtggag cgattccctc ttgtaagcag agaggtctag gagatccctc ccgatctcct
     1501 cctggaacct cgtcgccacc tcgaccagga gcctttccct ggccgttaag agctccctgt
     1561 gctgcaggtt ggcgaggatc ctcctgatat cctgttcgtt caccccgtca cccaccaatt
     1621 cctcgatgtg gttcgccctt tcctcgggca tatcgaggca gagtcttatg acccttatgt
     1681 acccatggcc gcctcgctgg ctctctacaa tgtagccccg ttctggggtg aaccggctcc
     1741 gtatcacgta atttatctgg ctcgggacac agccgaaacg ttcggcgagg tccttgcggc
     1801 tgagaaagac cgactcttcg ctgccccgtt ccagcagttc cacgatatgt ctttctatta
     1861 tcctcgtcag gcttgccaag gcgtttcctc ctttgacctt ctttgacaag tcaaattata
     1921 aggttaaggt caatattcgt caataataag gacgaccttt gggggattgt cacgacccta
     1981 tgggttcttc ttcccttttc accagtctga ggatgttcgt atggtggcgc gccacggcca
     2041 gcgctgccat ggccgccgag gtccagatat aagcgggggg gcccttgaag aggacaaacc
     2101 agaccgggac cgtcgcgagg gagaccatgg aaccgacgga aacgtacttc gatagtttca
     2161 taaccaggta ccaggccact cccccgatca aggccgggac ggggttccag aaaaagatca
     2221 cgccgaagga cgtggcgact cccttgccgc ccttgaaatt cagccagagg gggaagttgt
     2281 gccccaagac gccggcgaaa ccggccaggg ccagcaccaa gggctcctgt acgccgaaga
     2341 ggcgtgcggc gaggacgaca agccctccct tcgccatgtc gaagagggcc acggcgacgc
     2401 cccactttct gccggcgaaa cgggtaacgt tggtggcccc gatgttaccc gatccgaggg
     2461 tccttatatc cttccccagg agcatcctga cggcgaggta tcccgacggt atggacccga
     2521 aaaggtaccc agcgatcacc cacgcggcga ccgtcatcat cggggacccc tgattgcggc
     2581 cgcgtccacc gcgacggcgc ctccgccctc gtccagtacg aagacggggt tgatgtccag
     2641 ttccgccaag gactcttcgg cgagggccat cagcgaaacc cgggagacca tccctgccag
     2701 ggcctcggtg tccttggaga cgctcccccg agcgccggaa agcaggggga agcccttgag
     2761 ggaagctatc atcctgcggg cctcctcgac atccacgggg gcgtgcctca gggagaggtc
     2821 cttgaggatc tctacgtaga tgccgccgag gcccacggcg acgaagggcc cgaagacggg
     2881 gtcccttttc acaccgatca tgcattcaac gccgccggta accatctcct gcaccagcat
     2941 tccccggacc ctggccccgg gcatggcctt aagggacctc tcgagaagcc ggccgaaggc
     3001 gttcctgagt tcctcctcat ccttgagtct cagcgccacc gccccggcct cggtcttgtg
     3061 gattatgtcg ggcgacatta cctttagagc caccgggtag ccgatctccg aagcggctga
     3121 caggacgtcc tccagggaag agcagaggat ctccctcgtc accggcacgc cccaccgggc
     3181 caggagcctc ttggcgtcgt actcggtcag ttcctcgggc aaacggggta aaaaggggtc
     3241 tcccgggtcc gagggggccc tcgcgggaag ggcgggcctc gtcacccatt ccgaaagcct
     3301 ggctagggcc caggcacaac gcctggggct ggcgaagagg ggaactttct ccgacctgag
     3361 ttcctccagg aaggggccgc cctgttcgtt gtttataagc cagcagcaag ccatgggctt
     3421 ggaggctttc ctcgagactt cgatgatatc gcgggtgacg gaccaaccgg aatccccggt
     3481 gatcatggag atcacgacgg aggccatatc tatctccggg cagtccagga ccgtttcaag
     3541 ggatgacttg aagctcccgg ggtcatttat cacctgggcc gtgaggtcca cggggttcct
     3601 cgtggaaccg tagggtggga cgaaccgctc cagggcctcc ctggtctcgc ggggaagcgg
     3661 ctctactttc aaaccgaggt cataggccct gtcggccatg attatccccg cgccacccga
     3721 cgtggtgatc acaccaaccc tctttccgcg gggggttttc acggggccga agatcttacc
     3781 gaggtctgcg atgtcatcgg aatcctctat ttccagaact ccgtactgcc gaaaaattgt
     3841 cctccaggcc tcatcttcac ccgtaagggc cgcggtgtgg ctcttgaccg ccgcctgccc
     3901 cgtggggctt ctgc
//
LOCUS       DAOSRG010000091         3844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21342_length_3844_cov_6.157297, whole genome shotgun sequence.
ACCESSION   DAOSRG010000091 DAOSRG010000000
VERSION     DAOSRG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21342_length_3844_cov_6.157297"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..499
                     /locus_tag="PLM30_04215"
     CDS             152..499
                     /locus_tag="PLM30_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54404.1"
                     /translation="MRPGKFAATAAVLILVLSFAASGEAFLPGGFWRGDASLQAAGAF
                     ERVLEFFSRLLSEAGEGQGPDGGGAVFSKNPYVYIAAFWKEIVNAPRERTFIERVMAF
                     LEAVRVRQYRRSI"
     gene            767..2542
                     /locus_tag="PLM30_04220"
     CDS             767..2542
                     /locus_tag="PLM30_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOR54405.1"
                     /translation="MKIRTLDGPRLHRGFSAGANSIRARQGILNSMNVFPVPDGDTGT
                     NLSATVQSVSEGAVVSRSLSQTSASMADAALTGARGNSGLIFAQFLWGFSQGTEGLDE
                     LDVAAFGRAVSGAVPYAREALSKPVEGTILTVMEDWASEVAALSRRYDDFAHILPGSL
                     ETARRSLGETPSKLPVLAKAGVLDAGGQGFVDFLEGIVSFLESGDLRDLTLLSGAPTI
                     RHTHENFRNGEPAFRYCTEALLQGRGMDIGSIRADMQPFGDSLIVGGHGEKVRVHIHT
                     DTPDRLFFTIKKHGSLTQQKADDMRRQVDVCRNRMHSVALVTDSTCDLPQECLDRNQV
                     HVVPLRLAFGESVYLDRVTLSSDQFYTLLEGNGESPVSSQPPMSDFERTYRFLLEHYD
                     SVIAMHISSKLSGTWNASRAAAEKVGGKITVIDSRTASAPLGLLVMRAAEALNEGKGH
                     DEAVSLVEKSIPGAKIFISLRTLKNMVRGGRISPVKGFLASALNLKPIVFVDEEGYAR
                     SLGQTRGWERNVEKIRDIIDQERQKAPIWKYAIVHAHSPASADIAASGLAEIVGREPT
                     YVMDICPVLGAHTGLGSVAVGLLAE"
     gene            2550..2870
                     /locus_tag="PLM30_04225"
     CDS             2550..2870
                     /locus_tag="PLM30_04225"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HOR54406.1"
                     /translation="MAERREDNLTVEKKQVLERLAEVIEKTRVMANLEVLLDKRKLLW
                     NNFLAGLAKGLGFGVGLTVLAAIVIYFLVVLLKSLVALNLPIIGNHIVDLVQFVQEHI
                     SNQR"
     gene            2944..3543
                     /locus_tag="PLM30_04230"
     CDS             2944..3543
                     /locus_tag="PLM30_04230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOR54407.1"
                     /translation="MLIAALIILILASGGYLLWQRRPADFEAAQEPGGPGEAPPGQVS
                     QEGVPDPGEAAALVAANDLEGKFWLYIRKDRFELSAWNGNVRAALYTVAVGENPGDKE
                     RVGDRRTPEGVFRVESIHDSRAWVHDFGDGKGPVEGAYGPFFIRLDTGRWKGIGIHGT
                     HDPSSLGTRTTEGCIRMDNKALLEIVDKAVPGTVVVIEP"
     gene            3638..>3844
                     /locus_tag="PLM30_04235"
     CDS             3638..>3844
                     /locus_tag="PLM30_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2437 domain-containing protein"
                     /protein_id="HOR54408.1"
                     /translation="MKIIRFSVEGRNDPLWGVIEGDRVHVTSTPGGDRTGGSFPVALV
                     RLLAPALPTKIVCVGRNYHGHIREM"
BASE COUNT          749 a         1135 c         1238 g          722 t
ORIGIN      
        1 ctgctccccg aggcgaggga gcatgaaaaa cgccactcct tcctggcttt catggtgggg
       61 gtggccctcg cctcctttat cgtcttcacc cacggtcatt gatcgaattg ccggtcccac
      121 ttccggggca tggcgcgatg gaggtgatgg catgagaccg ggcaagttcg cggcaacggc
      181 cgctgtcctg atcctggtgc tatccttcgc cgcttccggt gaggcctttt tacctggtgg
      241 attttggcga ggcgacgcct ccttgcaggc tgcgggggcc ttcgaaaggg tcttggaatt
      301 cttcagccga cttctgtccg aggccgggga gggacagggc cctgacggcg gcggagcggt
      361 tttttccaaa aacccctacg tttacattgc cgctttctgg aaagagatcg tgaacgcccc
      421 tcgggagagg accttcatcg aaagggtgat ggccttcctc gaggcggtaa gggttaggca
      481 ataccggcga tccatctagc gatggagctc cccaaaggcc cgctcaaaaa cccgcggtct
      541 gccgcaccgg gaggggcccg caattcccgc cctttggcat atcctttctc ccatccatct
      601 ccggatgtca tgactcccct ctcacaccgt cgccgaccct tgtccccagc cacacggcgg
      661 gcgccggaag ttcttttccg acagacctgg atcgtttttt ctggtatcct tgtccgggta
      721 ttgccgcgag gggatacccg gatcccagaa cgcaagaggt gcgcgcatga agataagaac
      781 cctggatggg ccccgcctcc accgggggtt ttccgccggc gccaatagca tcagggccag
      841 gcaggggatc ctcaacagca tgaatgtatt cccggtgccc gacggagaca ccgggaccaa
      901 cctgtcggct accgtacaat ccgtttcgga gggggcggtg gtcagccggt ccctttccca
      961 gaccagcgcc tccatggccg acgcggccct cacgggggcc cggggcaact cgggcctcat
     1021 tttcgctcag ttcctgtggg gtttctccca gggaacggag gggctggatg aactcgatgt
     1081 ggcggccttc ggtcgggccg tctctggggc ggtcccctac gccagggaag ccctgagcaa
     1141 gcccgtggag ggtactatac tgacggtcat ggaggactgg gcttccgagg tagctgcgct
     1201 ctccaggcgc tacgacgatt tcgcccatat tctgccgggg tcgttggaga cggcccggag
     1261 atccctcggc gagacgccgt cgaaattgcc cgtcctggca aaggcgggcg tccttgacgc
     1321 cggcggccaa ggctttgtcg acttcctgga ggggatagtc tccttcctag agagcggcga
     1381 tctcagggac ctcaccctcc tttcgggggc tccgacgatc cggcacaccc acgagaactt
     1441 ccgcaacggt gagccggcct tcaggtactg tacggaggcg ctcctccagg ggagaggtat
     1501 ggacatcggg agcatcaggg ccgacatgca gcccttcggt gattccctca tcgtgggcgg
     1561 tcacggggaa aaggtgaggg tccacattca taccgacacc cctgacaggc tctttttcac
     1621 gatcaagaaa cacgggtcgc tcacccagca gaaggcagac gacatgagaa ggcaggtcga
     1681 tgtttgcagg aacaggatgc actccgtggc tctcgtcacg gactcgacct gtgacctgcc
     1741 ccaggagtgc cttgacagaa accaggtcca cgtggtgccg ctccgcctcg ccttcggcga
     1801 gagcgtctac ctcgaccggg tgaccctctc gtcggaccag ttctacaccc tccttgaggg
     1861 gaacggggaa agccctgtca gttcccagcc ccccatgtcc gatttcgaga ggacctaccg
     1921 tttcctcctg gagcactacg actccgtcat cgccatgcat atttcatcta aactcagcgg
     1981 gacctggaac gccagcaggg cggcggcgga aaaggtgggt gggaagatca ccgtcattga
     2041 ctcgaggacg gcctccgcgc cactgggact cctggtcatg agggccgccg aagcccttaa
     2101 tgaagggaag ggccatgacg aggccgtttc actggtcgaa aagagcatcc ccggggctaa
     2161 gatcttcatc agcctcagga ccctcaagaa catggtcagg gggggaagga tcagccccgt
     2221 caaggggttc ctggcgtccg cgctgaacct gaaacccatc gtcttcgtgg atgaagaggg
     2281 ttacgccagg tccttgggac agacacgggg ctgggaaaga aatgtagaaa aaatcagaga
     2341 tatcattgac caggagcgtc aaaaagcccc gatatggaaa tacgccatcg tccatgccca
     2401 ttccccggcg tcggccgata tcgccgcctc ggggctggcc gagatcgtag ggcgcgaacc
     2461 aacctacgtg atggatattt gcccggtgct gggggcccac actgggctgg ggtccgtggc
     2521 ggtggggctg ctggccgagt aggagcgcca tggctgaaag aagagaggac aacctcacgg
     2581 tagagaaaaa acaggtgttg gaacgccttg cggaagttat agaaaagacc agggtcatgg
     2641 ccaacttgga ggttctgctg gacaaacgga aactgctctg gaacaatttc ctggccggcc
     2701 ttgccaaggg gctcggcttc ggcgtgggcc tgacggtgct cgcggcgata gtgatctact
     2761 tcctggtggt cctgttgaaa tcgctggtgg cgctgaacct cccgataatc gggaatcaca
     2821 tcgtcgacct cgtccagttc gtacaggaac atatcagcaa tcaacgctga aaagggcggg
     2881 tcaaaaaaag gggctggtca tggcatgttc tccgccaggc gaaggccgag gctcttacca
     2941 aagttgctta tcgcggcgct gatcatcctc atcctggcgt cggggggata cctgctgtgg
     3001 cagaggaggc ccgccgactt cgaggccgcc caggagccgg gagggcccgg agaggcgccc
     3061 ccgggccaag tttcgcagga aggcgttccc gaccccgggg aggcggcggc cctggtcgca
     3121 gccaacgacc tggaaggtaa gttctggctg tacattcgaa aggaccgttt cgagctgtca
     3181 gcctggaacg gaaacgttag ggcggctctt tacaccgtcg cggtgggtga gaaccccggc
     3241 gacaaggaaa gggtgggcga caggagaacg cccgagggcg tcttccgggt cgagagcatc
     3301 cacgactcac gggcctgggt ccatgatttc ggcgatggca agggacctgt ggagggggcc
     3361 tacggtccct ttttcatccg actcgatacg ggccggtgga agggcatcgg tattcacggc
     3421 actcacgacc cctcctcgtt gggcaccagg accaccgagg ggtgtatcag gatggacaac
     3481 aaggccctgc tggagatcgt ggataaggcc gtgcccggaa cggtcgtggt catcgagccc
     3541 taaaatagcc gatccgggag gaatgccggg ggcggccctt cagagggccg gcgacaggtt
     3601 tttccggccc ccttgatgag aggcggtatc gattaaagtg aagataatcc gtttttccgt
     3661 ggagggaagg aacgatcccc tttggggcgt gatcgaaggc gacagggtcc atgtcacgag
     3721 cacccccggc ggggatcgga ccggggggtc cttccccgtc gcgctggtaa ggcttctcgc
     3781 gccggccttg ccgaccaaga tcgtctgcgt ggggcggaat taccacggcc acatccggga
     3841 gatg
//
LOCUS       DAOSRG010000092         3830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21441_length_3830_cov_8.926623, whole genome shotgun sequence.
ACCESSION   DAOSRG010000092 DAOSRG010000000
VERSION     DAOSRG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_3830_cov_8.926623"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLM30_04240"
     CDS             complement(<1..550)
                     /locus_tag="PLM30_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOR54409.1"
                     /translation="MRKTPLLALALLLCLAPAAFGAEAALSKTTLFVDAPWVKENASK
                     LIFVDARASSLYAKGHIPGAVNAEWTYFANVKGQPGNPGWGEIFPQDTLAKKIGALGI
                     NGKQPIVVYGDCGGWGQEGWAAWVFRLSGIDNVRILDGGYPAWLAAGGKVSTTAAKPK
                     AVAFSIKAFQGGYKVTTPWLASK"
     gene            complement(1125..1976)
                     /locus_tag="PLM30_04245"
     CDS             complement(1125..1976)
                     /locus_tag="PLM30_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR54410.1"
                     /translation="MFPEALKYSVAKSVNHAIKWLLNTHGSAFDHFSSGILGLLLRVQ
                     GVLVVIPWWAYLVVVALMAWIATKKILPTIILAALPLVIGIFGLWALAIETLALVITS
                     VVIALIIGIPLGIVMGELPLFAGFIQPVLDAMQTMPSFVYLIPAMMLFGLGKVPAVIA
                     TLIYSLPPVMRLTCLGLRQVPAPAQEAARAYGATRWQLLKEVRIPLAMPSILAGVNQT
                     TMMALAMVVIASMIGARGLGQEVLMAINRIDVGRGFEAGISIVIMGIVIDRITQGLAR
                     KWDPNNK"
     gene            complement(1966..2850)
                     /locus_tag="PLM30_04250"
     CDS             complement(1966..2850)
                     /locus_tag="PLM30_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163687.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HOR54411.1"
                     /translation="MENIIEVKDLWEVYSEKEVFFDVDDRDLIAALDESNESVVAVRG
                     VSFTVKPGEVFIVMGLSGSGKSTLIRCLLRLVEISWGEVRINGQDVTAMNMKELTEFR
                     RHEVAMVFQHYGLLPHKTVLENAAFGLKLRGVPKKERRERAYESLKAVGLEDWVDYYP
                     ASLSGGMLQRVGIARALVADTPILLMDEPFSGLDPLIRREMQDELLRIQQEYQKTIFF
                     VTHDLDEAMRLGDRMAVMRNGEIVQIGRPAEVFARPADDYVARFVQDKRDEIRRADEQ
                     MAAMAASQAERVGIGDVS"
     gene            complement(2860..3825)
                     /locus_tag="PLM30_04255"
     CDS             complement(2860..3825)
                     /locus_tag="PLM30_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR54412.1"
                     /translation="MTGLTSVPAAIAAERVVLVDFSWSSVQVHDRIAGFILEHGYGFS
                     PEYLFAESVPGLTGLQQGDVDIAMEVWVDNLLEWYTDAVEETGEVLDLGSIFPDAPQG
                     WYVPAYMIKGDEKRGIKATTPDLKSVFDLPKYWEAFKDPEVPGKGRFYNAIPGWVVHD
                     INLKKLETYGLSDSYTVFDPGSQMALQTAIVSAYEKGQPVLAYYWEPEWIMGLLDMVM
                     LEEPPYDPDRWGEGKDYGCAFPAARVHKGINTKFAEKHPEVVEFLEKYETTLAQNNGA
                     LAFMEQEEATVEEAAAWFLTNYKDVWKSWLPGDVAGKVEAALAKQ"
BASE COUNT          753 a         1146 c         1186 g          745 t
ORIGIN      
        1 gtttggaagc gagccagggc gtggtgacct tgtaacctcc ctggaaggcc ttgatcgaaa
       61 aggccacggc cttgggcttg gcggccgtgg tggaaacctt gccgccggcc gcgagccagg
      121 cggggtaccc gccgtcgagg atacggacgt tgtcaatccc ggaaaggcgg aacacccagg
      181 cggcccagcc ctcctgcccc cagccaccgc aatcaccgta gaccacgatg ggctgcttgc
      241 cgttaatccc gagggcgccg atcttcttgg ccagggtatc ctgggggaag atctcacccc
      301 aaccggggtt cccgggctgc cctttcacgt tggcgaagta ggtccactcg gcgttcaccg
      361 ccccggggat gtgccccttg gcgtaaagcg acgaagccct ggcgtcgacg aagatcaact
      421 ttgaggcgtt ctccttgacc cagggggcat ccacgaagag ggtggtcttc gagagggccg
      481 cctcggcccc gaaagcggcg ggggccaggc acaggagcag ggctagggct aaaagagggg
      541 tctttctcaa ggttatcatc ctttcctttc ggtgttgggg ggccggagtg cgaaaggacc
      601 ccgaccctgt tattttaacc tgcctaggct ctccggtcga tgggcatcaa accaagaatt
      661 ggggtcagac cttgaatttg agcaattgta taccaaaagg gcaaaatacg ggaacccgtg
      721 aatcggaaaa gccgtaaatc gtgagtcgtt aatcgtgaat cgaaaaggca aggagcataa
      781 gaccaaaggg cgtgagacgg gagacgtaag acggaaaggc aaggcaacag gcaatgggag
      841 gcaggcaacc ggagaggcgg aacccgggaa tcgaaggggc aagaaaccaa agggcgtgag
      901 aagttagacg ctagaggtaa aagaaaggcg cgggcaatgg gaaaaacgaa gggcatggat
      961 cggccagagc caaagtgttt ccggtaccat gcccttgagt cttctgcaga ctgtcgcctt
     1021 atgtctgctt tttttggcct tgagttcttc ctgccttctg actactgcta cctgacacct
     1081 tgtctttatg tatacaaatg ctcaaattca aggtctgacc ccaatcactt gttgttgggg
     1141 tcccacttcc tggcgaggcc ctgggtgatg cggtcgatga cgatacccat gatcacgatg
     1201 gagatccccg cctcgaaacc gcgacccacg tcgatgcgat tgatggccat gaggacttcc
     1261 tggccgaggc ccctggcccc gatcatggag gcgatgacga ccatggctag ggccatcatc
     1321 gtagtctggt tgaccccggc gaggatcgac ggcatggcca agggtatgcg aacctccttg
     1381 agaagctgcc accgggtggc tccgtaggcc ctggccgcct cctgggccgg ggcggggacc
     1441 tgccgcaggc ccaggcaagt gagtctcatc accggcggga gggaatagat cagcgtggct
     1501 attaccgccg ggaccttccc caggccgaag agcatcatgg ccgggatgag gtagacgaag
     1561 ctgggcatgg tctgcatggc gtccagcacc ggctggatga agcccgcgaa gaggggcagt
     1621 tcgcccatga cgatccccag aggtatcccg atgatgaggg ctatgaccac cgaggtaatc
     1681 accagggcca aggtctcgat agccagcgcc cagagaccga agatccctat gaccagggga
     1741 agggccgcga ggatgatcgt ggggagtatc tttttcgtgg ctatccacgc catgagggcg
     1801 acaactacca ggtaggccca ccagggtatg acgaccagga ctccctgcac ccgcaggagg
     1861 agccccagta tgccggagga gaagtggtcg aaggcggacc catgggtgtt gagcagccac
     1921 ttgatggcgt gattgacact cttagctacg gagtatttca gggcctcagg aaacatcgcc
     1981 gatccccacc ctttccgcct gggacgccgc catggcggcc atttgttcgt cggccctgcg
     2041 gatctcgtcc ctcttgtcct ggacgaagcg tgccacgtaa tcatcggcgg gccgggcgaa
     2101 gacctccgcc ggcctgccta tctggacgat ctcgccgtta cgcatcacgg ccatcctgtc
     2161 gccgagccgc atggcctcgt cgaggtcgtg ggtgacgaag aatatcgtct tctggtactc
     2221 ctgctggatc ctcaggagtt cgtcctgcat ctcccgtcgg atgagggggt caaggccgct
     2281 gaagggctca tccatcagga ggatcggggt gtcggccacg agggcccggg ctatgcccac
     2341 cctctggagc atgcctccac tcagggaagc cgggtaatag tcgacccagt cctcgaggcc
     2401 gaccgccttc aggctctcgt aagccctctc gcggcgctcc tttttcggca cgcccctgag
     2461 cttgagcccg aaggccgcgt tctccaggac cgtcttatgg gggagcaggc cgtagtgctg
     2521 gaagaccatc gccacctcgt ggcggcggaa ctcggtgagt tctttcatat tcatggccgt
     2581 tacgtcctgg ccgtttatcc ggacctcgcc ccaggatatc tcgaccagcc tcaggaggca
     2641 ccggataagt gtcgatttgc cgctcccgga gaggcccatg acgatgaaga cctccccggg
     2701 tttgacggtg aaggatacgc cccggacggc gacgaccgat tcgttggatt cgtccagcgc
     2761 cgctatcagg tcccggtcgt ccacgtcgaa aaagacttcc ttttccgagt agacctccca
     2821 gagatccttc acttctataa tattttccat cgtgagcgtc tactgtttcg ccagggctgc
     2881 ctcgaccttc ccggccacgt cgccggggag ccatgacttc cagacatcct tgtaattggt
     2941 gaggaaccag gcggcggcct cctccacggt ggcctcctcc tgctccatga aagccagcgc
     3001 cccgttgttc tgggccagcg tggtctcgta cttttccagg aactccacga cctcggggtg
     3061 cttttcggca aacttggtgt tgatgccctt gtgcaccctg gcggcgggga aggcgcagcc
     3121 gtagtccttg ccctcacccc accggtcggg gtcgtagggc ggttcctcga gcatcaccat
     3181 gtcgagcaac cccatgatcc actcgggctc ccagtaatag gcgagcacgg gctgcccctt
     3241 ttcgtaggcc gatacgatgg ccgtctggag ggccatctgg gaaccggggt cgaaaacggt
     3301 gtaactgtcg gatagcccgt aggtctcgag ttttttcagg tttatgtcgt gcaccaccca
     3361 cccggggata gcattgtaga agcgcccttt accgggcacc tcgggatcct tgaaggcctc
     3421 ccagtacttg ggcaggtcga atacggactt gagatcgggg gtcgtcgcct ttatcccccg
     3481 cttttcgtcg cccttgatca tgtaggcggg gacgtaccac ccctggggcg cgtcggggaa
     3541 gatggagccc aggtcaagga cctcgccggt ctcctcgacg gcgtcggtgt accattcgag
     3601 cagattgtcc acccacacct ccatggctat gtccacgtcg ccctgctgca ggcccgtgag
     3661 gcccggcacc gactcggcga agaggtactc cggggagaaa ccatagccat gctccagtat
     3721 gaagccggcg atacggtcat ggacctgtac gctgctccag ctgaagtcaa ccagtactac
     3781 cctttcggct gcgattgcgg cgggaactga ggtcagcccc gtcacgacgg
//
LOCUS       DAOSRG010000093         3828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21455_length_3828_cov_10.279618, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000093 DAOSRG010000000
VERSION     DAOSRG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21455_length_3828_cov_10.279618"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLM30_04260"
     CDS             <1..846
                     /locus_tag="PLM30_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR54413.1"
                     /translation="GTKIMTTSSSPGISLMSEGISYIAGCEIPAVIVNVMRGGPGLGG
                     ILPSQADYMQATKGGGNGDYRLLVLAPSTLQEAVELTQMAFDLAQQYRNPVMILADGF
                     MGQMMEAVEIKEHISENLPPREPWALGCMKERGGKRAILKSLYLNPEDLERHNIELQK
                     KYRGMEKEVRFEAYRCDGAEILLCAFGTTARIARSAINNLRNEGIKVGMLRPITLYPF
                     PKEKIRELATGLKGVLVVEMNMGQMVEDVEAAVCGKAPVSFFGHAGGIAPSVKEIEEQ
                     IRAVL"
     gene            867..1613
                     /locus_tag="PLM30_04265"
     CDS             867..1613
                     /locus_tag="PLM30_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR54414.1"
                     /translation="MKEIKIYERPKSWAKDVHTHYCPGCGHGITHRLICEIIDELGIQ
                     DDVIGTAPVGCAALMYDYFDIDFCEAAHGRAPALATGIKRTRPDKIVFTYQGDGDLAS
                     IGTAEIIHVANRGEKLTTIFINNAIYGMTGGQMAPTTLIGQKTTTSPFGRDPELAGYP
                     MRMCELLATLETPGFITRVSVAKPKYIIEAKKAIKQAFVNQIEGKGYSFVEVLSNCPT
                     NWGMTPVQAFEWTESAMIPHYPLGVYKNFE"
     gene            1627..2190
                     /locus_tag="PLM30_04270"
     CDS             1627..2190
                     /locus_tag="PLM30_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR54415.1"
                     /translation="MSGFFRGMIAAGFGGQGIMVLGQLVAYTAISEGRHVTWIPSYGP
                     EMRGGTANCGVAISDEEIASPVVAEADVTVIMNNPSMERFEGSVKPGGLLVYNSDLVK
                     YPNPRKDVTIFPIPAHSVALALGSDKVSNIVVLGAIVEATDLVGLEACIETIKDKLGK
                     RKPQFLPMNLEAFEKGREIARELLKKA"
     gene            2194..2721
                     /locus_tag="PLM30_04275"
     CDS             2194..2721
                     /locus_tag="PLM30_04275"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR54416.1"
                     /translation="MFPREEKVSGELIYKGRILNLRVDRVRLPDGGMALREVVEHAPA
                     VGIIAETGDGGLFLVRQYRYPAGEFLLEIPAGIVEPGEEPAETADRELREETGFKPGA
                     LAEVAQFFTSPGFSDERIILFHARELSPAPKECDSDEFMDLVRLDRRQLQDAALSGEI
                     RDSKTLTAIYWFLSR"
     gene            2775..3662
                     /locus_tag="PLM30_04280"
     CDS             2775..3662
                     /locus_tag="PLM30_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOR54417.1"
                     /translation="MKRDTPDLATFFNYLRYERGCSENTVSAYRSDLRGWFSFCSEKG
                     IPASPPLPERIAEYLGFLSREGRSKSSIQRAAAALRSWTRFLASEGVSGGPLDLLRLP
                     ARERKLPKVLTEGEVDRLLEACEGDDPISLRDRAIVEVGYGCGLRAGEIASLDLADVG
                     LHTGLIRLRGKGDKERVVPLVGKVKGRVERYLSSGRGLLGGSGGGKFFLSRNGRPLRR
                     EDVWRIIQKRGEKAGISRSRLHPHILRHSFATHLLRRGMDLRVLQELLGHSSIMTTEK
                     YTHFDVELRDVYDKSHPRA"
BASE COUNT          819 a         1104 c         1241 g          664 t
ORIGIN      
        1 gggacgaaga tcatgaccac ctcgtcgagc ccggggatat ccctcatgtc cgaggggatc
       61 agttacatag ccgggtgcga gatccccgcc gtcatcgtca acgtcatgag gggaggcccc
      121 ggcctaggcg ggatactccc gtcccaggcg gactacatgc aggccacaaa gggtggcggt
      181 aacggggact accgtctcct ggtcctcgcc ccgagcacgc tgcaggaagc cgtggaactg
      241 acccagatgg ccttcgacct cgcccagcag taccgcaacc ccgtcatgat cctcgccgac
      301 gggttcatgg gccagatgat ggaggccgtc gagatcaagg agcatatctc ggagaacctc
      361 ccgcccaggg agccctgggc gttggggtgc atgaaggagc gcgggggcaa gagggccata
      421 ctcaaaagcc tctacctcaa ccccgaagac ctggagcgcc acaatattga attgcagaaa
      481 aagtaccgcg ggatggaaaa agaggtcagg ttcgaggcat accgctgcga cggcgccgag
      541 atcctgcttt gcgcctttgg gacgacggca aggatagcga gatcggccat aaacaacctg
      601 aggaacgaag gcataaaggt cggcatgctg aggcccataa cactgtaccc cttcccgaag
      661 gaaaagatcc gggaactcgc caccggtttg aagggcgtcc tggtggtcga gatgaacatg
      721 gggcagatgg tcgaggatgt cgaggcggcc gtctgcggga aggccccggt gtcctttttc
      781 gggcacgcgg ggggtatcgc tccctcggtc aaggagatcg aggagcagat cagggccgtt
      841 ctttagtcca gggaggtgtc ggaccgatga aagagatcaa gatctacgaa aggccgaagt
      901 catgggccaa ggacgtgcac acccattact gcccggggtg cgggcatggc atcacccaca
      961 ggctcatctg tgagatcatc gatgagctgg ggatacagga cgatgtgata gggaccgcgc
     1021 ccgtcggttg cgccgccctt atgtacgact acttcgacat cgacttctgc gaggcggccc
     1081 acgggagggc gccggcgctg gccacgggca taaaacggac ccggccggac aagatcgtct
     1141 tcacctacca gggggatggt gacctggctt ccattgggac ggccgagatc atccacgtgg
     1201 ccaacagggg agagaagctg accaccatct tcattaacaa cgcaatctac gggatgaccg
     1261 gcggccagat ggccccgact accctcattg ggcagaagac taccaccagc cccttcggga
     1321 gggaccccga actggccggt taccccatga ggatgtgcga actcctcgcc accctcgaga
     1381 ccccgggttt catcacgagg gtctccgtgg ccaagcccaa gtacatcatc gaggccaaga
     1441 aggccatcaa gcaggccttc gtgaaccaga tcgagggcaa gggttactcc ttcgtggagg
     1501 tcctctccaa ctgcccgacc aactggggga tgacaccggt ccaggctttc gagtggaccg
     1561 agagcgccat gatacctcac taccccctgg gtgtttacaa gaactttgaa taggggaggg
     1621 aaggatatgt ccggattttt cagaggaatg atcgccgccg gtttcggggg gcagggcata
     1681 atggtcctgg gccagctggt ggcctacacg gccatcagcg agggccgtca cgtcacctgg
     1741 atcccctcct acgggcctga gatgcgcggc gggaccgcga attgcggcgt ggccatcagc
     1801 gacgaggaga tcgcctcgcc cgtcgtcgcc gaggcggatg tcaccgtgat aatgaacaac
     1861 ccgtcgatgg aaaggttcga ggggtcggta aaacccggag ggctcctggt gtacaactcc
     1921 gacctggtca aataccccaa ccccaggaag gatgtcacca tcttcccgat accggcccac
     1981 agcgtggccc tggccctcgg cagcgacaag gtctccaaca tcgtcgtgct gggggccatc
     2041 gtcgaggcca ccgacctggt gggcctcgag gcctgcatcg aaaccataaa ggacaaactc
     2101 gggaaaagaa aaccccaatt tctccccatg aacctggagg ccttcgaaaa ggggagggag
     2161 atagcccggg aactgctcaa gaaagcctga gccatgttcc cccgggagga gaaggtttcg
     2221 ggcgaactga tatacaaggg gcggatcctt aacctccggg tagacagggt caggctcccc
     2281 gacgggggca tggcgctgcg ggaggtggtt gaacatgccc ccgccgtcgg cattatcgcc
     2341 gaaaccggcg atgggggcct cttcctggtg aggcaatacc ggtaccccgc tggggagttc
     2401 ctcctggaga tccccgcggg gatcgtcgaa cccggtgagg agccggccga gacggccgac
     2461 agggagctga gggaggagac cgggttcaag ccgggggctt tggcggaggt ggcccaattc
     2521 tttacttcgc cgggcttcag cgacgaaagg atcatcctct ttcacgcccg tgagctttca
     2581 ccggcgccca aggagtgcga cagcgacgaa ttcatggacc tggtcaggct tgaccggcgg
     2641 cagctccagg atgcggccct ttccggggag atcagggatt ccaagacgct gacagctatt
     2701 tactggttcc tttcgagata aaagccaaaa cgggggaggg gctccgggcc cctccccggg
     2761 aaggccgacc gagtatgaaa agggataccc ccgaccttgc tacgtttttc aattacctcc
     2821 gctacgagag gggttgctcg gagaacaccg taagcgccta ccgcagtgac cttcgcggct
     2881 ggttctcctt ttgctcggaa aaaggcatcc ccgcttcgcc gcctctgccg gagaggatag
     2941 cggaatacct gggctttctc tccagagagg ggcgcagcaa gtcatcgatc cagagggcgg
     3001 cggcagcgct ccggtcctgg acgaggttcc tcgcctcgga gggtgtttcc ggtggacctc
     3061 tggacctttt acgccttccc gccagggaga ggaagctccc caaggtcctg acggaggggg
     3121 aggtagacag gcttctcgaa gcctgcgagg gcgatgaccc catctctctc agggacaggg
     3181 ccatcgtcga ggtcggttac ggctgcggcc tcagagcggg agagatagcc tcgctggacc
     3241 tcgccgacgt ggggctccac acggggctga tcaggctcag gggcaagggg gacaaggaga
     3301 gagtggtccc ccttgtcggg aaggtcaagg gaagggtcga gaggtacctg tcctccggga
     3361 ggggtttgct gggtggttcc ggaggcggta agtttttcct ctccaggaac gggaggccct
     3421 tgagaaggga ggacgtctgg cggatcatcc agaaaagggg tgagaaagcc ggcatctccc
     3481 gctcaaggct tcacccgcac atactgaggc attccttcgc gacccatttg ctcaggcggg
     3541 gtatggacct cagggtcctt caggaactgc tgggccattc ctcgataatg accacggaga
     3601 aatacactca ttttgacgtg gaactccggg atgtttacga taaaagccac cccagggctt
     3661 gaacttcgcc gcgaaaaaat gtatgcctga ggaggttgcc cattgtctta cagggaagag
     3721 gtcgccagag cagcgaaatt aattcaggac aggatccgtg cggtgcccaa ggtcgccatc
     3781 gtgctgggct ccggactggg atccctggcc gattcgttgg aggacgcc
//
LOCUS       DAOSRG010000094         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21529_length_3819_cov_10.918438, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000094 DAOSRG010000000
VERSION     DAOSRG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21529_length_3819_cov_10.918438"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PLM30_04285"
     CDS             complement(<1..1547)
                     /locus_tag="PLM30_04285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOR54418.1"
                     /translation="MSERTIGELCEALVRLREEAEAGGGEKAVAKQHEKGKLTARERI
                     DKLLDEGSFVELDTFVEHRCIHFGMGKKTYPGDGVVTGYGTVDGRLVYVYSQDFTVIG
                     GSLGEMHARKICKVMDLAMQNGVPVLGLNDSGGARIQEAVDSLSGYGNIFYRNTVASG
                     VVPQLSLILGPTAGGAVYSPALTDFIFMVDKIGILHITGPDVIKAVTGEDVTSEQIGG
                     AMAHNQISGNAHFFATTEDECFAQVRKLLSYLPSNNMEEPPFYDSGDDPYRTDESLRD
                     LVPTNPNKGYRVQDVLTRIADGGEFFEVQAMWAKNIVTGFMRVGGYVAGVIANQAAIM
                     AGCLDIDASDKASRFIRYCDAFNIPIVTFVDVPGYLPGKNQELGGIIRHGAKMLYAYS
                     ESTVPLITVVLRKAYGGAYLGMCSKDLGADVVLAWPQAEIAVMGAEGAANIIFRREIE
                     GSPDPQALRAQKIEEYREAFATPYQAAKRGFVDRVILPEETRTEIYRALVQSEGKRQQ
                     RPKRKHGV"
     gene            complement(1576..1998)
                     /gene="mce"
                     /locus_tag="PLM30_04290"
     CDS             complement(1576..1998)
                     /gene="mce"
                     /locus_tag="PLM30_04290"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOR54419.1"
                     /translation="MKPTAIDHIGIAVASLDEALKFWEQSLGIKCTGVEEVAEQKVRT
                     AFLPLGDTEVELLEATDPESPVAKFIEKRGEGIHHLAIRVENLEESLQKMKALGLRMI
                     DEKPRYGAGGARIAFVHPKTAGGVLLELSERPGLDSGR"
     gene            complement(2046..2462)
                     /locus_tag="PLM30_04295"
     CDS             complement(2046..2462)
                     /locus_tag="PLM30_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOR54420.1"
                     /translation="MNERRIRVIVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PESIVETAIQEDVDAIGVSILSGAHEHYFREIIRLLKEKNAEDIILFGGGVIPEGDIA
                     GLTGMGVGAIFGPGTPTGTCIEWLRDKVRDKRSRES"
     gene            complement(2491..>3819)
                     /locus_tag="PLM30_04300"
     CDS             complement(2491..>3819)
                     /locus_tag="PLM30_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOR54421.1"
                     /translation="PTQIGYDSDHPMARGEVGKVGVAIDSLADMETLFREIPLAKVST
                     SMTINAPAAVLLAMYIAVGEKQGVPASELSGTIQNDILKEYIARGTYIFPPKPSMRLI
                     TDIFGFCSDNVPKWNTISISGYHIREAGSTASQEVAFTLADGLAYVQAAVAAGLDPDV
                     FGQRLSFFFNAHNDFLEEVAKFRAARRLWARLMKERIGVTEPKALMLRFHTQTAGSTL
                     TAQQPENNIVRVAIQALASILGGTQSLHTNSFDEALALPTEKSVGIALKTQQIIACES
                     GSTSTVDPLAGSYAIEALTNEIERQTLEYIDKIEEMGGMLAAIEKGYPQQQIQGAAYQ
                     YQKSVESGERVVVGVNKFQVDEENGGRELLRVDPSVGEGQEKALKAMKQGRDNVLVGN
                     RLEAIRSAAGDESVNLMPAIIDAVKVYATEGEICGVLREEFGEYRENIVL"
BASE COUNT          598 a         1215 c         1171 g          835 t
ORIGIN      
        1 acgccgtgct tgcgtttcgg gcgctgctgg cgcttgccct cgctctggac aagggcccgg
       61 tagatctcgg ttcgggtctc ctcagggagg atcaccctgt ccacgaagcc cctcttggcg
      121 gcctggtagg gcgtggcgaa ggcctcccgg tactcctcga tcttctgcgc cctaagggcc
      181 tggggatcgg gggagccttc gatctccctc ctgaagatta tgttggcggc gccctcggcg
      241 cccatgacgg ctatctcggc ctggggccag gcgaggacca cgtcggcgcc gaggtccttg
      301 ctgcacatcc ccaggtaggc cccaccgtag gccttgcgga ggaccaccgt gatgaggggc
      361 accgtggact cgctgtaggc gtagagcatc ttcgcgccgt gcctgatgat cccccccagt
      421 tcctggttct tgccggggag gtagccgggg acgtcgacga aggtgacgat ggggatgttg
      481 aaggcgtcgc agtaacggat gaaccggctg gccttgtcgg aggcgtcgat atcgaggcag
      541 ccggccatta tggcggcctg gttggctatg acccccgcga cataccctcc gactcgcatg
      601 aaaccggtga cgatgttttt ggcccacatg gcctggacct cgaagaactc gccgccgtcg
      661 gcgatcctgg taaggacgtc ctggaccctg tagcccttgt tcgggttggt cgggacgagg
      721 tccctcaggc tttcgtcggt gcggtagggg tcatcgccgc tgtcgtagaa gggcggctcc
      781 tccatgttgt tggaaggcag gtaggagagc agctttctta cctgggcgaa gcactcatct
      841 tcggtggtgg cgaagaagtg cgcgttgccg gagatctggt tgtgggccat ggcccccccg
      901 atctgctcgc tggtcacgtc ctcgccggtg acagccttga tgacgtcagg cccggtaatg
      961 tggaggatgc ctatcttgtc gaccatgaag atgaagtcgg tcagcgcggg gctgtacacg
     1021 gcaccgccgg cggtggggcc caggatgagc gacaactggg gcacgacgcc gctggcgacg
     1081 gtgttgcggt agaagatgtt gccgtaacca ctcaggctgt cgacggcttc ctggatcctc
     1141 gcaccgccgc tgtcgttcag ccccaggacg gggaccccgt tctgcatcgc caggtccatg
     1201 accttgcaga tcttcctggc gtgcatctcg cccagggaac cccctatgac ggtgaaatcc
     1261 tggctgtaga cgtaaaccag gcgcccgtcg acggttccgt agccggtgac cacgccgtcg
     1321 ccggggtagg tcttttttcc catcccgaag tgtatgcacc ggtgttcgac aaaggtatcg
     1381 agttcgacga aactcccctc gtcgaggagt ttgtcaatcc tctccctcgc cgtcaacttg
     1441 cccttttcgt gctgcttcgc gacggccttt tcgccgcccc ccgcctcggc ttcctcacgg
     1501 agcctgacca aggcctcgca aagttcaccg atcgtcctct cagacatctc ttttcctccc
     1561 ttttgcatat tcccgttatc ggccgctatc caaccctgga cgttcgctca gttccagcag
     1621 cacacccccc gccgtcttgg ggtgtacaaa ggctatcctc gcaccccccg ccccgtatct
     1681 cggcttctcg tcgatcatcc tgaggccgag ggccttcatc ttctggaggg actcctccag
     1741 gttctcgaca cggatggcga ggtgatggat cccctcacct cttttttcga tgaacttcgc
     1801 aacggggctt tcagggtcgg tggcctcgag gagttccacc tcggtgtcgc cgaggggcag
     1861 gaaggccgtc ctgaccttct gctccgccac ttcttcgacc cccgtgcact tgatccccag
     1921 cgactgctcc cagaacttga gagcttcgtc gaggctggca acggctatgc cgatatgatc
     1981 tatcgctgta ggcttcatga taaccctccg atctggggcg aggccggccc tcgcccggtt
     2041 tttccctagg attccctgga cctcttatcc cgtactttgt ccctgagcca ctctatgcag
     2101 gtgcccgtgg gggtcccggg gccgaagatc gccccgacgc ccatcccggt caagccggct
     2161 atatctccct cggggatcac acctccgccg aagaggatga tgtcctcggc gttcttctcc
     2221 ttcaagagcc ggattatctc acggaaatag tgctcgtggg ctccgctcag gatgctcacc
     2281 cctatggcgt cgacgtcctc ctggatggcg gtttccacta tgctctcggg ggtctgcctg
     2341 aggccggtgt agatgacctc catgcccgcg tccctgagag ccctggccac caccttggcc
     2401 cccctgtcat gaccatcaag gccaggcttg gccacgataa cacggatcct gcgttcgttc
     2461 atttccgtcc ctcctgacac caggcgtccc ttaaaggacg atattctccc tgtattcccc
     2521 gaactcttcg cggagaacgc cgcagatctc gccctccgta gcgtagacct tcaccgcgtc
     2581 gataatggcc ggcatgaggt tgacgctttc gtcgccggcg gcggatcgga tcgcttcgag
     2641 tcggttgccg acgagcacgt tgtcacgccc ctgcttcatg gccttcaggg ccttctcctg
     2701 gccttcgccc acggaagggt ccacccggag gagttcccgg ccgccgttct cctcatcgac
     2761 ctggaacttg ttgacgccca ccacgacgcg ttcgccgctc tcgacggact tctggtactg
     2821 gtaagccgcg ccctggatct gctgctgggg atagcctttc tcgatggccg ccagcatgcc
     2881 gcccatttct tctatcttgt cgatgtactc cagggtctgg cgctctatct cgttggtgag
     2941 ggcctcgatg gcatagctcc cggcgagggg gtccaccgtc gaggtagacc ctgattcgca
     3001 ggcgatgatc tgctgagtct tgagggctat gcccacgctc ttctccgtgg gcagggcaag
     3061 ggcttcgtcg aagctgttgg tgtggaggga ctgggtccct cccaagatgg aggccagggc
     3121 ctggatcgcg acgcgtacga tgttgttctc cggctgctgg gctgtgaggg tgctccccgc
     3181 cgtctgggtg tggaagcgaa gcatgagggc cttcggttcc gtgacgccga tccgttcctt
     3241 catgagcctt gcccaaagcc tcctggcggc ccggaacttg gccacttcct cgaggaaatc
     3301 gttgtgggcg ttgaagaaaa aggagagccg ctggccgaat acatcgggat caagcccggc
     3361 ggccaccgcc gcctggacgt aggcaagacc gtcggccagg gtgaaggcaa cttcctgcga
     3421 ggccgtggag ccggcctccc ttatatggta gcccgaaatg gatatggtgt tccacttggg
     3481 gacattgtcg ctgcagaagc cgaagatgtc ggtgatgagg cgcatggagg gtttgggtgg
     3541 gaaaatgtag gtgccgcggg cgatatattc cttgaggatg tcgttctgga tcgtaccgga
     3601 cagttccgac gccggcaccc cctgcttctc gccgacggcg atgtacatgg ccaaaaggac
     3661 cgccgccggg gcgtttatcg tcatcgacgt ggagaccttg gccaggggga tctccctgaa
     3721 gagggtctcc atgtcggcca ggctgtcgat ggcgaccccg accttgccga cctcgcccct
     3781 tgccatgggg tgatcggaat cgtaccctat ctgggtcgg
//
LOCUS       DAOSRG010000095         3818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21540_length_3818_cov_8.676056, whole genome shotgun sequence.
ACCESSION   DAOSRG010000095 DAOSRG010000000
VERSION     DAOSRG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21540_length_3818_cov_8.676056"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PLM30_04305"
     CDS             <1..1583
                     /locus_tag="PLM30_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR54422.1"
                     /translation="AVVSNWGVFYSRRDMNPTSIPDLDGMKVAVVKRDTYGEAFKSLA
                     ARFGITCSYLEVDDYPDVFKAIEGYKAEAGLVSRLFGLTNEAEHRVDRTNIFLEPREL
                     RFAAPKGLSVTVLQILDFHLTKWRPIPDSPLNRSIQNWLSERSVMSVIPRWFPWFVGA
                     SLATVLGVLFTNLMLHRKVLARSRELLKSKADLSREKVLFEKLFEESPDALVLQSTED
                     NSILRVNRGFTRLFGYRRDEAEGRTLNEMIVPGDLLQEGIEMDRRTDKGNALQEETVR
                     RKKSGALVDVSLISVPVTLEGGLVGAYVIYRDISAAKEARKALIESREHAEKSLESMS
                     RAWEQTIDVLAMAAETRDPYTAGHQRNVSALSEAIAKELWLDKETVNSIRMAGLVHDV
                     GKINIPTEILSKPGTLGEVEFALIRTHPEVGYEIMKNIELPWRLADMILQHHERMDGS
                     GYPRGLKGDRIMLEAKVLAVADVVEAMSSHRPYRASLGVEAALEEIETNKGRLYDPQV
                     ADACLRLFREKGFRFPGS"
     gene            1617..2066
                     /locus_tag="PLM30_04310"
     CDS             1617..2066
                     /locus_tag="PLM30_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR54423.1"
                     /translation="MMRKALVAIDGSDITKPLVRFAFERARKEKLQGLDFIYVFQPYP
                     AALFPGMPIPTTIEEEHAKATRKALEAMVLKEREEAGAEKVEVSMVFPYGSPSGEIIR
                     QAEEYPYRSIFIGHRGMGSLERFFIGSVAARVVEHAPCTVIVFRPRD"
     gene            complement(2041..>3818)
                     /locus_tag="PLM30_04315"
     CDS             complement(2041..>3818)
                     /locus_tag="PLM30_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOR54424.1"
                     /translation="VDHPSFANAVIPRVLEARGYRVGVIAQPDWRSLEDFLALGRPRL
                     GALVSAGNLDSMLSHFTAAKKPRRDDPYSPGGKAGGRPQRATVVYCNRAREAWKDIPL
                     IIGGVEASLRRFAHYDFWSDSVRRSVLVDAKADLLVFGMGEKPILEIAEALSRGVPVG
                     MIRDVPGTCRRAKGTDLPPGAVVLPSFEEVSRDRAAFAEAFRLQYGEQDPFRGCPVAQ
                     DQGAWYVIQNPPAMPLSTEEMDWVYELPYRREPHPMYGEKGGVPAVEEVRFSITAHRG
                     CFGSCSFCSITSHQGRVIQARSDASILREAKTLTGLPGFKGYIHDVGGPTANFHSPAC
                     SAQLSRGSCPDRDCLWPEPCAALETDQKGYFELLKKVRSLPGVKKVFVRSGIHYDYLL
                     AARDPRLIEDLCEHHVSGQLKVAPEHVSPRVLALMRKPGKEVFLEFMREFDRANRKSG
                     KEQYLVPYLMSSHPGSTLADAVELALFLKEIRYHPEQVQDFIPTPGTLSTCMFYTGLD
                     PMTGEEVYVPRSAHERKLQRALLQYRSPANRKLVEEALVKAGREDLIGTGPHCLVGPM
                     GRGVRQKGRSDKGRRTRLSRGGGKR"
BASE COUNT          704 a         1197 c         1258 g          659 t
ORIGIN      
        1 aggccgtcgt atccaactgg ggggtcttct actcccgccg tgacatgaac cccacctcca
       61 tccctgacct ggacggcatg aaggtcgccg tggtcaaaag ggacacctac ggggaagcct
      121 tcaagagcct cgcggcgcgc ttcgggataa cctgctccta ccttgaagtg gatgattacc
      181 ccgatgtgtt caaggccatc gaaggctaca aggccgaggc cgggcttgtg agccgcctct
      241 tcgggctgac caacgaggcg gagcaccggg tcgacaggac caacatcttc ctggagcccc
      301 gggaactgcg cttcgccgcc cccaagggcc tcagcgtgac ggtcctgcag atcctcgatt
      361 ttcacctcac caagtggagg cccatccccg attcccccct caacaggtct atccagaact
      421 ggctctccga gaggagcgtc atgagcgtca ttccgcggtg gtttccctgg ttcgtggggg
      481 cgtccctggc gaccgtgctg ggtgtcctct tcacgaacct gatgctgcac aggaaagtcc
      541 tggcccgctc cagggaactc ctgaagagca aggccgatct ttccagggaa aaggtcctct
      601 tcgagaagct cttcgaggaa tcgcccgatg ccctggtgct gcaaagcacg gaggacaaca
      661 gtatcctgcg ggtcaacaga ggcttcacca ggctcttcgg ttaccgccgc gacgaggccg
      721 agggcaggac tctgaacgaa atgatagtgc ccggggacct gctccaggaa gggattgaaa
      781 tggacagaag gaccgacaag ggcaacgcct tacaggaaga gacggtgagg cggaagaaga
      841 gcggcgccct cgtcgatgtc tccctcattt ccgtgcccgt cacgctggag ggggggctcg
      901 tcggcgccta cgtcatatac agagacatat cggcagcgaa ggaggcaagg aaggccctca
      961 tcgaaagccg tgagcatgcc gaaaaaagtc tcgagagcat gagccgcgcc tgggaacaga
     1021 ccatcgacgt cctcgccatg gccgccgaga cccgtgatcc ctataccgcc ggtcaccaga
     1081 ggaatgtctc tgccctttcc gaagccatcg ccaaggagct gtggctcgac aaggagaccg
     1141 tcaattccat caggatggcg gggctggtcc acgacgtggg caagataaac ataccgacgg
     1201 aaatactgag caaacccggt accctcggcg aggtggaatt cgccctgatc aggacccacc
     1261 ccgaggtggg ctacgagata atgaagaaca tcgagcttcc ctggcgcctg gccgacatga
     1321 tcctgcagca ccatgagagg atggacggct ccggttaccc gaggggactc aagggggacc
     1381 ggatcatgct cgaggccaag gtcctcgccg tggctgacgt ggtggaggcc atgtcctccc
     1441 atcgccctta cagggcctcc ctgggggtgg aagccgccct ggaggagata gagaccaaca
     1501 aggggcgcct ctatgacccc caggtggccg acgcctgcct gaggcttttc agggagaagg
     1561 gtttccgctt tcccggatcc tgatcgagga tccgggggct atcatggagg tgatcgatga
     1621 tgcgcaaggc gcttgtggcc atcgacggaa gcgacataac gaaaccgctg gtgaggttcg
     1681 cctttgagcg ggccaggaag gaaaagctcc aggggctcga cttcatctac gtcttccagc
     1741 cctaccccgc cgcgcttttc ccggggatgc ccatccccac cacgatcgag gaggagcacg
     1801 ccaaggccac caggaaggcc ctggaggcca tggtgctcaa ggagcgggag gaggccggag
     1861 ccgaaaaggt cgaggtctcc atggtcttcc cctacgggag cccttcgggg gagataatcc
     1921 gccaggcgga ggagtacccc taccggtcca tcttcatagg ccacaggggc atggggagcc
     1981 tggagaggtt cttcataggc agcgtggccg ccagggtggt ggagcacgcc ccctgcacag
     2041 tcatcgtttt ccgcccccgc gactgagccg ggtccgcctc cccttgtcgg agcgcccctt
     2101 ctgcctgacc ccgcggccca tgggcccgac caggcagtgg gggcctgtac cgatgaggtc
     2161 ctccctcccc gccttgacca gggcctcctc gaccagtttc ctgttggccg gcgagcggta
     2221 ctgcagcagc gccctctgga gcttcctctc gtgggccgag cggggcacgt agacctcttc
     2281 gccggtcatg gggtccaggc ccgtgtagaa catgcacgtt gaaagggtcc ccggcgtggg
     2341 gatgaaatcc tgcacctgct cggggtggta acggatctcc ttcaggaaca gggccagttc
     2401 cacggcgtcg gccagggtgg agccggggtg gctcgacatc aggtagggga ccaggtactg
     2461 ctccttcccc gacttcctgt tggccctgtc gaattccctc atgaactcca ggaagacctc
     2521 cttaccgggt ttcctcatca gcgccaggac cctgggcgag acgtgctcgg gggccacctt
     2581 gagctgaccg ctgacgtggt gttcgcaaag gtcctcgatg agcctcgggt cacgggcggc
     2641 caggaggtag tcgtagtgga taccggagcg cacgaaaacc ttcttcaccc ccgggaggga
     2701 gcggaccttc tttaaaagtt cgaaatagcc cttttggtcg gtttccaggg ccgcgcaggg
     2761 ttccggccag aggcagtccc tgtcggggca ggagccccgg gaaagctggg cggagcaggc
     2821 gggggagtgg aagtttgccg tgggtccccc cacgtcgtgg atataaccct tgaaaccggg
     2881 cagccccgtc agggtcttgg cctccctgag gatggacgcg tcgctcctgg cctggatcac
     2941 cctcccctgg tgggaggtta tggagcagaa ggagcagctt ccgaagcagc cccggtgggc
     3001 cgttatgctg aaccggacct cctcgacggc ggggacgccc cccttttcac cgtacatggg
     3061 gtggggttcc ctcctgtagg ggagttcata gacccagtcc atctcctccg tggaaagggg
     3121 catggccggc gggttctgaa tgacgtacca ggccccctgg tcctgggcga cggggcaccc
     3181 ccggaagggg tcctgttccc cgtactggag ccgaaaggct tcggcgaagg ccgcgcggtc
     3241 gcgggagacc tcctcgaagg agggcaggac gacggccccc ggagggaggt ccgtcccctt
     3301 cgcccggcgg caggtccccg ggacatccct tatcatcccc acgggcacgc ccctggaaag
     3361 ggcctcggcg atttcgagga tcggtttttc acccatgccg aagacgagga ggtccgcctt
     3421 ggcgtcgacg aggacggacc tcctgacgct gtcggaccag aagtcgtagt gggcgaacct
     3481 cctgaggctg gcctcgacgc cgcctatgat gagggggatg tccttccagg cctcccgggc
     3541 ccggttgcag tagaccaccg tcgccctctg gggccttccc ccggccttgc cgccggggga
     3601 ataggggtcg tcacggcggg gttttttcgc cgccgtgaag tgggagagca tggagtccag
     3661 gttgcccgcc gagaccaggg cgcccaggcg gggcctgccg agggccagga agtcctccag
     3721 gctcctccag tcgggctggg cgatcacgcc gacccggtag ccccgggcct cgaggaccct
     3781 gggtatgacg gcgttggcga aactggggtg gtcgacgt
//
LOCUS       DAOSRG010000096         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21624_length_3806_cov_8.753399, whole genome shotgun sequence.
ACCESSION   DAOSRG010000096 DAOSRG010000000
VERSION     DAOSRG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21624_length_3806_cov_8.753399"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /gene="argS"
                     /locus_tag="PLM30_04320"
     CDS             complement(<1..1627)
                     /gene="argS"
                     /locus_tag="PLM30_04320"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOR54425.1"
                     /translation="MKKDIETVIKEMLVLSIGQISRERGISLEGGPPVEVERGRREGQ
                     GDWATNAAMQWAGAFGERPLEMARRIVSLLPENPLVERVEVAGPGFINFFLSRSWMSG
                     TIEHILELAGDYGRSDSGGGRRVQVEFVSANPTGPLHVGHGRGAAVGDICARILSFTG
                     WDVEREYYINDAGLQMELLGRSTQSRYFEICGRPGEVPFPEDGYKGGYIFDLARQLLD
                     SEGERYLDIPPGESLPFFTRYASEVILEDIRKTLENFGVKFDVWFSEKSLYDGVKIPK
                     MLETLKKNGFAFDADGATWFKATAFSDDKDRVLIRSNGVPTYFASDIAYHKNKYDRGF
                     DMVIDVWGADHHGYIPRMKAAVQALGRKEEDLQILLIQFVNLHRGGQQVSMSTRSGEF
                     VTLEEVIDEVGVNATRYFFAMRRSDSHLDFDLDLAKSASTDNPVFYVQYAHARIHSIF
                     REAAARGVAMPKPSEVDLSLLVEPEETSLVKRLSAFPGEVEKAAADLAPHRIVFYAYD
                     LAGDFHSFYNAHRVLGVGPELEGARLVLSEATRV"
     gene            complement(1647..3461)
                     /locus_tag="PLM30_04325"
     CDS             complement(1647..3461)
                     /locus_tag="PLM30_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54426.1"
                     /translation="MRSRPFFFALVLLIAFTFTGESQGQDIPALLSKTLDEAPSLQAL
                     GSGKGTVVLRDMNYRLLADGKTERTTTIFLYEGENLPDSWRNWEIPIPVGGEASVLES
                     ALYDPYSRRVRFPLIPREIERDGVRMIEVRLPNDFEDNILVLSYREVLPTRMNLEDAV
                     PVDLDLAQWEQRITLAVPSGVEPAWLGENLPAPQVSRDGVEDVYTWSIINTPAYEGAS
                     LSPGKGRWLIFSLRKGIQYSLSDAASLADSLDTPPPPRVATLLKDPDRSRRGISIMEY
                     MNDPARLDRSLPPELVRVPAEIPAEGPWTEWEASFLLGNWLLKAGWGVETCWDSRAPL
                     SDGSPATVKAWGKPVLSVKPAEGKPFFFEIGQAVRPGSMPPRLWGRTLYSLDGGTPSR
                     KTLPSGGPGDHRLSFNWALEVAADGMATGELTVTAGGAWVEVLAGAADKDAAAEILAS
                     FSWPSIPGISFQEATVETPGTGLRVKVPLHVRIAIPGGEGLLLRMPSVVMPWQRSIAE
                     KRVAGEIRFPFVFEQTAGIVLPEGFKVMVLPATRPYDAGQVKLEESMRVKKGHTLVAE
                     QKTVVSTARLEEQAQQALANVVRQQLGWNEATVPLQKR"
     gene            complement(3510..>3806)
                     /locus_tag="PLM30_04330"
     CDS             complement(3510..>3806)
                     /locus_tag="PLM30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOR54427.1"
                     /translation="LLEYQFESYNLFQEMMERVRLSFTQYFFRVTVATPGSAPARSTA
                     SRRESRDAVAPLTAGEPGDAPRPQPVRSTPKVGRNQPCPCGSGKKYKHCCGKNA"
BASE COUNT          712 a         1205 c         1118 g          771 t
ORIGIN      
        1 ctaccctcgt cgcttcggag aggacgagcc tggcgccctc cagttccggt cccaccccca
       61 ggacccggtg ggcgttgtag aaggagtgaa aatccccggc caggtcgtag gcgtaaaaaa
      121 cgatcctgtg aggggcgagg tcggcggcgg ccttttcgac ctccccgggg aaggccgata
      181 gcctcttcac gaggcttgtc tcctccggtt cgaccagcaa agagaggtca acctccgatg
      241 gtttaggcat ggccacgccc ctggccgccg cttccctgaa aatactatgt atcctcgcgt
      301 gggcgtactg gacgtaaaaa accgggttgt ccgtggaggc actcttggcc aggtccaggt
      361 caaaatcaag gtggctgtcg gaacgcctca tggcgaaaaa gtacctcgtg gcgttgaccc
      421 ccacctcgtc gatgacctct tccagagtga cgaactcgcc ggagcgggtc gacatggata
      481 cctgctgccc tccccggtgg aggtttacga attggatcag gaggatctgg aggtcttcct
      541 ctttcctgcc gagagcctgc accgcggcct tcatccttgg gatgtaaccg tgatggtcgg
      601 ccccccacac gtcgatcacc atgtcaaaac cgcgatcgta cttgttcttg tgataggcga
      661 tatcggaggc gaagtatgtg gggaccccgt tgctcctgat gaggacacgg tccttgtcgt
      721 ctgagaaggc cgttgccttg aaccaggtcg ccccgtcggc atcgaaggcg aaaccgttct
      781 ttttgagggt ctccagcatt ttggggattt tgaccccatc gtagagggac ttctcagaga
      841 accagacgtc gaacttcaca ccgaaattct caagggtctt gcggatatcc tccaggatga
      901 cctcactggc gtacctggta aaaaaaggga gggactcccc gggcgggatg tccaggtatc
      961 gctctccctc cgagtccagc agttgccggg cgagatcgaa aatgtacccg cccttgtacc
     1021 cgtcctccgg gaaggggacc tccccggggc gaccacagat ctcgaagtac cgggactggg
     1081 tcgacctccc caggagttcc atctggagcc cggcgtcgtt gatgtagtac tccctttcga
     1141 catcccaccc tgtgaaggaa aggatcctcg cgcagatgtc acccacggcg gccccccggc
     1201 catgccctac atggaggggt cccgtcgggt tggcgctcac gaactccacc tgtacccttc
     1261 gcccgccgcc cgaatcactc ctgccgtaat cgcctgccag ttccagtatg tgctcaatgg
     1321 tcccggacat ccacgaccgg gagaggaaaa agtttatgaa acccggtccc gccacctcga
     1381 ccctctccac gagggggttt tccggcagaa gggagactat cctgcgggcc atttcaaggg
     1441 gtctctcccc gaaggcaccg gcccactgca tggcggcgtt ggtggcccag tcaccctggc
     1501 cttcccttct tcccctctcc acctccacgg gaggacctcc ctcaagggat atccccctct
     1561 cccgggagat ctgcccgatc gacaatacaa gcatttcctt tatgacggtc tcaatatcct
     1621 ttttcatggt ctcgccccgc tttcggtcac cttttttgca aaggcactgt ggcctcgttc
     1681 cagccgagct gctgtcgtac gacgttcgcc agggcttgtt gtgcctgctc ttccagcctg
     1741 gccgtcgaga caacggtctt ctgctccgcc accagggtat gtcccttctt taccctcatg
     1801 gactcctcca gcttgacctg ccccgcgtcg tagggccggg tcgccggaag taccatgacc
     1861 ttgaaaccct cggggaggac aatgccggcg gtctgttcaa agacaaaggg gaaacggatc
     1921 tctcccgcaa cccttttttc ggctatcgaa cgttgccagg gcatcactac cgagggcatc
     1981 ctgagcagta gcccctctcc gccggggata gcgatcctga cgtggagggg taccttgacc
     2041 ctcaaacccg tcccaggcgt ttccaccgtc gcctcctgga aggagatccc cgggatcgaa
     2101 ggccaggaaa aggaggccag gatctcggct gcggcgtctt tatcggcggc gccggcgagg
     2161 acctctaccc aggcgccccc ggctgtgacc gtcagctccc ccgtggccat gccgtccgct
     2221 gcgacctcca gggcccagtt gaaggaaaga cggtggtcac cgggccctcc cgagggcaac
     2281 gtttttctcg atggggtgcc cccatcgagg gaatacaggg tcctacccca gagccgaggc
     2341 ggcatggagc ccggccttac ggcctgtcct atctcgaaaa agaagggttt cccctcggcc
     2401 ggcttaaccg acaggaccgg tttcccccat gcctttaccg tggcgggtga cccatccgag
     2461 aggggggccc gcgagtccca gcaagtttcc acgccccagc cggccttcag tagccaattt
     2521 cccaggagga acgaggcctc ccactctgtc caggggcctt ccgccgggat ctcggcgggc
     2581 accctgacga gttcgggggg gagcgaacgg tcgaggcggg cggggtcgtt catgtactcc
     2641 atgatactga tccccctcct cgatctgtcg gggtccttca agagggtcgc cacccgcgga
     2701 ggaggaggag tatcgaggga gtcggccaac gaggcggcgt cggagagtga gtactggata
     2761 cccttcctga ggctgaatat aagccagcgc cccttccctg gagaaaggga ggccccttcg
     2821 taggcagggg tgtttatgat gctccaggta tagacatcct cgacaccgtc cctgctcacc
     2881 tgtggagcgg gaaggttttc gcccagccag gccggttcaa ccccggaggg caccgccagg
     2941 gtgatcctct gttcccactg ggcaaggtca aggtctaccg gcacggcgtc ctcgaggttc
     3001 atcctcgtgg ggaggacctc tcggtaggac aagacgagga tgttgtcctc gaagtcgttg
     3061 gggagcctca cctcgatcat ccggaccccg tccctttcta tctcccttgg gatcagggga
     3121 aacctgaccc ggcggctata aggatcataa agggccgatt ccaagaccga tgcctcgccc
     3181 ccgacgggaa tgggaatctc ccaatttctc cagctgtcgg ggaggttttc gccttcatag
     3241 aggaaaatgg tggtggtcct ttccgttttc ccgtcggcca ggagtcggta attcatatcc
     3301 ctcaggacca cggtcccttt ccccgatccc aaggcctgca aggaaggggc ttcatccaag
     3361 gtctttgaga gaagggcggg gatatcctga ccttgggatt ccccggtaaa ggtgaaggct
     3421 atcagcagta ccagcgcgaa aaagaagggt ctcgatctca aaaaaatcac tccctcatgc
     3481 tcctgggtgc aggacggtcg atacggtcct caggcattct tcccgcagca gtgcttgtac
     3541 tttttcccgc tgccgcaggg gcatggctgg ttccttccga ccttcggggt actcctcacc
     3601 ggttgcggcc tgggcgcgtc accgggttca ccggcggtga ggggcgctac cgcatcgcgg
     3661 gactcccgac gggaggccgt tgacctggcc ggagccgatc ccggtgtcgc cacggtcacc
     3721 ctgaagaagt actgggtgaa ggagagccgg acccgctcca tcatctcctg gaaaagatta
     3781 taggattcaa actggtattc aagaag
//
LOCUS       DAOSRG010000097         3778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_21831_length_3778_cov_9.832393, whole genome shotgun sequence.
ACCESSION   DAOSRG010000097 DAOSRG010000000
VERSION     DAOSRG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21831_length_3778_cov_9.832393"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1593)
                     /locus_tag="PLM30_04335"
     CDS             complement(232..1593)
                     /locus_tag="PLM30_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOR54428.1"
                     /translation="MTVPEKGISRRCAEFVTSLDHSDLGRETVSVTKKCIVDWLGCVL
                     GGASTPAAGIIKGVVNEMGGREQATMAGDFSKTSVLQAATVNAYNCHILEMDDVHKSA
                     VVHPAAPVISAAFALGEYLGSSGKEVIEAIVAGYDVMIRIGEAVMPSHYEIWHSTATC
                     GNFGAAASSARLLKLDATQTLYSLGNAGTQAAGLWEFASDNAMTKYLHCGKAAYNGVL
                     SSLMAKRGFDGATRILEGDRGFFRAYSRESDFERSFEDMGRKYKIDETVYKPYASCRH
                     THGPINGVLVLRSRMGFSWEDVESVTVETYGTALKLAGNRDHSTPPAARFSLAYCLAA
                     AIVFGKVGVEQFREEILDDPRITAVTPRIRIEATPEMDAMHPEKWPSRTTVKLKNGDS
                     ETLFVEYPKGDPENTMTDEEIREKYLALSALAIPEEKARLLLDRCYSIEERESMADFF
                     QGL"
     gene            complement(1640..3208)
                     /locus_tag="PLM30_04340"
     CDS             complement(1640..3208)
                     /locus_tag="PLM30_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOR54429.1"
                     /translation="MIGSGGAGLTAAISSAEEGCSVTVLSKTATGAASATSYSSGFFT
                     FPDEDLSAGEYRDLVRGVGKNLSREDLLEALGEQARDCLLQLRSWGATLRFLGGGHAT
                     VIDSARVPMTSGSGLVSELMDIASDKGVTFLENVFVTRLLVEGGRVFGVDHLDWTTGE
                     AGRLFSKAVILATGGAGQIFLRNDTPARVTGDGYALALESGLALEDMEFIQFYPLGFD
                     EPRFPCWMIRLPVLDLARLTDGSGREFLREEMKARGLRDGMAVSLFARDMAARLIQEK
                     LADGGEVLLHLEDIERSRWGEWDLRKSLGCYPHGVTPWEYGPVHVSPLAHYFSGGVAI
                     DARASTSIEGLFAAGEVTGGVDGASRVGGNALTGMVFSGITAGRGAARLALSASPRPF
                     KPGEGPQKSGFTRGRGPSPRALKGEIKRLNQECLGPLREEGSILRGLSTLEEYRRSLP
                     AMTIEKPTDLLEALELRSMILASLAVGYSALSREESRGVHFRTDHPREVPGFERPQEV
                     RIEGGRFVTRFKDR"
     gene            complement(3320..>3778)
                     /locus_tag="PLM30_04345"
     CDS             complement(3320..>3778)
                     /locus_tag="PLM30_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HOR54430.1"
                     /translation="NFSLGTTGGALVVSLVFGALGRVGPLNMRMDKGILAALRSFSLA
                     YFFATVGLQAGPQVAETFAEYGILLISIGFFAALISELVAFLVGRYLLHLNWILLAGA
                     ICGAMTSTPGLGAAIDATGSEDCGAGYGATYPFALLCMVLFTKLLVSTFR"
BASE COUNT          702 a         1226 c         1123 g          727 t
ORIGIN      
        1 agctggggca caaccccgtg gcggcccttt actgccccta catccgctcg gaacttggca
       61 ggcccctaag ggaaaggtcc gtagaaaaaa gcggtgccac caccgtcgtc gaccatctga
      121 ggggaaaata aagccccggc cttcccgggc cggggctttc cagttgacga tgttgcctgt
      181 cctttcctgg ggaagggcac actgcccttc ttccgctacg gttgccgttt cttaaagacc
      241 ctggaagaaa tccgccatgg actcccgctc ttcgatggag tagcaccggt cgagtaggag
      301 gcgggccttt tcttctggaa tggccagggc ggaaagggcg aggtactttt cccttatctc
      361 ctcgtcggtc atagtgttct ccgggtcgcc cttgggatat tcaacgaaaa gcgtctcgct
      421 gtcgccgttt ttcagcttca ccgtcgtcct ggagggccat ttctcggggt gcatggcgtc
      481 catctcgggg gtcgcctcga tcctgatcct cggggtgacc gcggtgatac gcgggtcatc
      541 gaggatctcc tccctgaact gttcaacccc caccttaccg aaaacgatgg ccgcagcgag
      601 gcaataagcc aggctgaacc tggcggcggg gggtgtcgag tgatcccggt tgccggcgag
      661 cttcagcgcg gtgccgtagg tctcgacggt gacgctttcc acgtcctccc aggaaaaacc
      721 catcctggaa cggagcacca ggaccccgtt tatcgggcca tgggtatggc ggcatgaggc
      781 gtagggcttg tagaccgtct cgtcgatctt gtactttctc cccatgtcct cgaaagacct
      841 ttcaaaatcg gactccctcg aataggccct gaaaaagccg cggtcgccct cgaggatcct
      901 cgttgccccg tcgaagcccc ttttggccat gagggaggac agcacaccgt tgtaggcggc
      961 cttcccgcag tgaaggtatt ttgtcatggc gttgtcggag gcgaactccc aaaggcccgc
     1021 ggcctgggtc ccggcgttgc ccagggagta cagggtctgc gtggcgtcca gtttcaggag
     1081 cctggccgag gaggcggcgg ccccgaaatt gccgcaggtg gccgtggagt gccatatctc
     1141 gtagtgcgag ggcatgaccg cctcccctat ccgtatcatc acgtcgtaac cggccacgat
     1201 ggcctcgatg acctctttcc ccgaactgcc caggtattcc ccgagggcga aggcggccga
     1261 gatgaccggg gcggcggggt ggacgacggc acttttgtgt acatcgtcca tttccaggat
     1321 gtggcagttg taagcgttca ccgtggctgc ctggagtacg gaggtcttcg aaaaatcccc
     1381 ggccatggtg gcctgctccc gcccgcccat ttcatttacg acgcccttga tgatccccgc
     1441 ggcgggtgtg gaggcccccc ccaggacgca cccgagccag tccacgatgc atttctttgt
     1501 caccgagacc gtttcccggc ccaggtcgga atgatcaagg gacgtgacga attccgcgca
     1561 tcttctgctt attccctttt cagggacggt catggaaaac acccctttcc atacttagaa
     1621 acggcaagcc cttttcaggt taccggtcct tgaagcgggt gacgaatcgt cccccttcga
     1681 tcctcacctc ttggggcctt tcaaacccgg gcacttctct cgggtggtcc gtcctgaaat
     1741 ggacgccgcg gctctcctcc cttgaaaggg ccgaatagcc caccgcgagg gaggcgagga
     1801 tcatgctcct caactcaagg gcctcgagca gatcggtcgg cttttctatt gtcatagccg
     1861 ggagcgatcg tcgatactcc tcaagggtgg agaggcctct caggatcgat ccttcttctc
     1921 tcaagggacc caggcattcc tggttgagcc tcttgatctc gcccttcagc gccctggggg
     1981 aagggccacg tccccgggtg aacccgcttt tttgcggccc ttcgcctggt ttgaaggggc
     2041 gcggtgaggc ggagagggcg agccgggccg ctcccctccc ggccgtgatg ccggaaaaaa
     2101 ccatcccagt cagggcattg cccccgaccc tgctcgcgcc gtccaccccc ccggtcactt
     2161 caccggctgc gaaaagcccc tcgatggagg tcgacgcccg ggcgtcgatg gcaacacccc
     2221 cgctgaagta atgcgccagg gggcttacgt ggaccgggcc gtattcccag ggagtgaccc
     2281 cgtgcgggta acagccgagg cttttcctga ggtcccactc accccagcgg gatctttcga
     2341 tatcctccag gtgcagcagg acctcgccgc cgtcggccag cttttcctgg atgagccttg
     2401 ccgccatatc cctggcgaag aggctcacgg ccattccgtc ccggaggcct ctcgccttca
     2461 tctcctcccg gaggaactcc cttcccgacc cgtcggtcag gcgggccagg tccaggacgg
     2521 ggagtcttat catccagcag gggaaacggg gctcatcaaa acccaggggg tagaactgga
     2581 tgaactccat gtcctcgagg gccaggccgg actcaagggc gagggcgtac ccgtcgccgg
     2641 tcaccctggc tggagtgtcg ttcctcagaa agatctggcc tgctcccccg gtggcgagta
     2701 tcacggcctt gctgaagagc ctgccggcct cgcccgtggt ccaatccagg tgatccacgc
     2761 cgaagaccct gcctccctcg accaggagcc gggtgacgaa aacattctcg aggaaggtga
     2821 cacccttatc ggaggcgatg tccatgagtt ccgacacaag gccggagccg ctggtcatgg
     2881 gtacccgtgc cgagtcgatc accgtcgcgt gcccgccgcc gagaaaacgg agggtggcgc
     2941 cccaggaacg cagttgaagc aggcaatcac gggcctgctc ccccagggcc tccaggagat
     3001 cctccctgct gagattcttt ccgactcccc tgacaagatc cctgtattca cccgcgctga
     3061 gatcttcatc gggaaaggtg aaaaaaccgc tggaatagga cgtggcggaa gccgccccag
     3121 tagcggtctt gctcaggaca gtaacggaac aaccttcctc cgccgaggag atggccgctg
     3181 taaggcccgc tcccccggaa ccgatcacaa ctacgtcggt atgaatgatc cccttccccc
     3241 ctcgcgaaag ccgcccactt ttgaccgttt ttgcggggtt ccccgttacc tgccggggcg
     3301 ctcttcccgg caattcaaac tagcggaaag tgctgaccag caacttcgtg aacaggacca
     3361 tgcagagcag ggcgaagggg taggtcgccc cgtatccagc cccgcagtcc tcgctcccgg
     3421 tggcgtctat ggccgccccg agccccggcg tgctcgtcat tgcgccgcag atcgcgccgg
     3481 cgaggaggat ccagttcagg tggaggaggt acctccccac gaggaaggcg accagctccg
     3541 atatcagcgc ggcgaagaaa cctatgctta tgagaaggat cccgtattcc gcaaaggtct
     3601 ccgccacctg aggtcccgcc tgaagaccca cagtggcgaa aaaataagcc agcgagaagg
     3661 aacggagggc ggccaggatc cccttgtcca tacgcatatt caagggcccc acccttccca
     3721 gggcgccgaa aacaagggac acgaccaggg ctccccctgt cgtccccagg gagaaatt
//
LOCUS       DAOSRG010000098         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22138_length_3736_cov_10.551752, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000098 DAOSRG010000000
VERSION     DAOSRG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22138_length_3736_cov_10.551752"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1035)
                     /locus_tag="PLM30_04350"
     CDS             complement(103..1035)
                     /locus_tag="PLM30_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOR54431.1"
                     /translation="MIVTVTLNPAVDEECLVPEFRPGKWFRAGQVNRSPGGKGINVSY
                     MLSQIDYPSVAMGFLAGFNGEYIRDALRRLHITTNFIHVPGETRSNVYIVDEVGHIET
                     GVAESGPYIPEEALGRFLSNYERMLVRARFVMIGGSLPPGVPQDIYRDLVERAKSKGI
                     PTLLDAAGPPLLAAIEAGPTFARVDHRFMAWVAGISLTSLDNLIEVVSRVHDYGVEWA
                     VASYRNYGAVFFSPEGIFLAEYERKGLASLFASDDALVAGFMIAREEKMSAEDTIRLS
                     TACAWENSLRVEKGFRGRSEIDQLATQVQVEKLD"
     gene            complement(1099..2286)
                     /gene="trxB"
                     /locus_tag="PLM30_04355"
     CDS             complement(1099..2286)
                     /gene="trxB"
                     /locus_tag="PLM30_04355"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOR54432.1"
                     /translation="MGKRELVIIGAGPAGLTAAIYGKRSGLDVLVLEKGIPGGQINST
                     GEIENWTGVLHASGAELADSFRVHAEHFKAEFLETEVSKITVDGGRKVIHTDKGEIEA
                     EAVIIATGASFRRLGAPGEKEFTGRGVSYCAVCDGPFFEDQEIAVIGGGNTAVEEAVY
                     LTQFASKVYIIHRRDSFRADRMATERALTNPRIEVLWNSVVEEIAGEDMLEKLVLKNV
                     RTGEISELPVSGVFVFTGMDPNISFMEGLVESAKGGWVLTNERMETSVEGVFAAGDVR
                     DKYLRQVITAAGDGATAAMAASSYISEQLHLQGVLLEPEQVTAYFFSSIDEAQARLAT
                     KIEESAGGGTKKVVLLDGYKNKRMVEKLGITNLPVIVEFRKGRIQRSKQVGSLEEAVE
                     FLS"
     gene            complement(2342..3190)
                     /gene="prmC"
                     /locus_tag="PLM30_04360"
     CDS             complement(2342..3190)
                     /gene="prmC"
                     /locus_tag="PLM30_04360"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOR54433.1"
                     /translation="MNLREVRKEAAARLAEAGIDSPRLEADLLIRHITGWERASLLAH
                     PERLFPDGLFPVLVEALERRAAREPLQYITGVCEFMDRTFQVGPGCLIPRPETELLVL
                     ESRKYFREGIFLDWGTGSGCIAASILLENPESRCVAVDGSPRAIAWAWRNFKERGLLG
                     RSLLCHSPSFERIPMPPDGFRMIVSNPPYIPTEVIPDLMPEVSRYEPLYALDGGDDGS
                     DHYRSLARWARTALAPGGTLIVEMGSDEQAEELISVTRGAMTVLSVVRDLQGSARVVV
                     LKRSPF"
     gene            complement(3194..>3736)
                     /locus_tag="PLM30_04365"
     CDS             complement(3194..>3736)
                     /locus_tag="PLM30_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOR54434.1"
                     /translation="ESGVHRVQRVPVTESSGRIHTSTATVAVLPEAEDVDVEVRQEDL
                     KIDTYRASGAGGQHVNMTDSAVRITHLPTGLVVTCQDERSQIKNRIRAMNFLRAKLYD
                     LELRQQQEKMAAERKGQVGTGERSERIRTYNFPQNRVTDHRIGLTLHRLDSMLEGDLQ
                     EMISALVMADQAEKLRSMEG"
BASE COUNT          634 a         1215 c         1104 g          783 t
ORIGIN      
        1 ttgaggaaat gcctcgcgaa cccttcggcc atcgggctcc tgcaggtgtt gcccgagcag
       61 acgaacacta cctctctcga ggggtacagg ctgtccatgg gatcagtcga gtttttcaac
      121 ctggacctga gtcgcgagct ggtctatctc cgatcggccg cggaagccct tttcgacgcg
      181 gagggaattc tcccaggcgc aggccgtgga taagcgtatg gtatcctcgg cgctcatctt
      241 ttcctccctg gcgatcatga aaccagcgac cagggcgtca tcggaggcga acagcgaagc
      301 caggcccttt cgttcgtact ccgcgaggaa gatcccctcg ggggagaaga agacggcgcc
      361 gtagttcctg taggacgcga cggcccattc gaccccgtaa tcgtggaccc gggagaccac
      421 ctcgatgagg ttgtccaggg aggtgaggga aataccggcc acccaggcca taaaacggtg
      481 gtccaccctg gcgaaggtcg gacccgcctc gatggcggcc agaagcggcg ggcccgccgc
      541 gtcgaggagg gtggggatcc ccttggactt ggccctttcg accaggtccc tgtagatatc
      601 ctgggggacc cccggcggga gggagccccc gatcataacg aaccgggccc tgacgagcat
      661 cctttcgtag ttggagagga atctgccgag ggcctcctcg gggatgtagg ggccgctttc
      721 agctacgccg gtttcgatat gccccacctc atcgacgatg tagacgttcg aacgggtctc
      781 accggggaca tggatgaagt tggtggttat gtgaagcctc ctgagggcgt ccctgatgta
      841 ctcgccgttg aaccccgcca ggaaacccat cgccaccgag gggtagtcga tctgggataa
      901 catgtaggag acgttgatgc ccttgccgcc cggtgacctg ttgacctggc cggcccggaa
      961 ccacttcccc ggtcggaact ccggtacgag gcactcttcg tccacggccg ggttcagcgt
     1021 tacggtcact atcatggttc gcacctcccc tgaggatcgc gaaggcaatt accgccccga
     1081 gaggcggggc cggggccttt acgacaggaa ctccaccgcc tcttccaggg accccacctg
     1141 cttcgaccgt tggatccttc ccttccggaa ctccactatg acggggaggt tcgtgatgcc
     1201 gagcttttcg accatcctct tgttcttgta gccgtccagg aggaccacct ttttggttcc
     1261 gccgccggcg ctctcctcga tcttcgtggc aagccgggcc tgcgcctcgt cgatgctgga
     1321 gaagaagtag gccgtgacct gctccggttc caggaggaca ccctggagat gcagctgctc
     1381 cgatatatag gaagaggcgg ccatggcggc cgtagcgccg tccccggcgg cagtaattac
     1441 ctgcctcagg tacttgtccc tgacgtcgcc ggcggcgaag acaccttcaa cgctggtctc
     1501 catcctttcg ttggtcaaaa cccatccgcc tttcgcggat tcgacgaggc cttccatgaa
     1561 ggatatgttg gggtccatgc cggtgaagac gaagaccccc gagacaggca gttccgatat
     1621 ctcaccggtc ctgacgttct tgaggacgag cttttcgagc atgtcctccc cggctatctc
     1681 ctcgacgacc gagttccaca ggacctctat cctggggttc gtcagggctc tctccgtggc
     1741 catccggtcg gcgcggaagc tgtccctccg gtggatgatg tacaccttcg atgcgaactg
     1801 ggtcaggtag acggcctctt ccacagcagt gttgccgccc ccgatgacgg ctatctcctg
     1861 gtcctcgaaa aagggcccat cgcagactgc gcagtagctg acgcccctgc cggtgaactc
     1921 cttctccccc ggtgcaccca gcctcctgaa actcgccccg gtggctatga tgacggcctc
     1981 ggcctcgatc tcccccttat cggtatggat gacctttctc ccgccgtcga cggtgatctt
     2041 gctgacttcc gtttcaagga actcggcctt gaagtgttcg gcgtgcaccc tgaagctgtc
     2101 ggccagttcg gccccgctgg cgtgcaggac gccggtccag ttctcgatct cgcccgtgga
     2161 gtttatctgt cctcccggta tccccttttc aaggacgagg acgtcgagcc ccgatctctt
     2221 cccgtagatg gcggccgtga gacctgccgg tcccgctcct atgatcacga gttccctttt
     2281 tcccatcgac cattcctcct gaacgtaagg ttttatctcc gtgaaaactc accatattgt
     2341 atcagaaggg cgacctcttc aaaacgacca ccctggcgct tccctgcagg tctctgacga
     2401 cggacagcac cgtcatcgcc ccccgggtca ccgagatcaa ctcctccgcc tgctcgtcgc
     2461 tacccatctc aacgatgagg gtcccccccg gcgcgagggc cgtcctcgcc caccgcgcca
     2521 gggaccggta atggtcggac ccgtcatcgc ccccatcgag ggcgtaaaga ggctcataac
     2581 ggctgacttc gggcatcagg tccgggatca cctcggtggg gatataaggg gggttgctga
     2641 cgatcatcct gaaaccgtcc ggcggcatgg ggatcctctc aaaggacggg ctgtggcaga
     2701 gaaggctcct tcccaggagg cccctctcct tgaagttcct ccatgcccag gcgatggccc
     2761 tggggctgcc gtctacggcg acgcacctgc tttcggggtt ctcgagcagt atcgaggcgg
     2821 cgatgcatcc cgaaccggtg ccccagtcca ggaaaatccc ctcccggaag tacttcctgc
     2881 tttccagcac aagcagttcc gtctcggggc gggggatcag gcaacccggg ccgacctgaa
     2941 aagttcggtc catgaattcg cagacaccgg ttatgtactg aaggggttcc ctggcagccc
     3001 tcctttccag ggcttccacc aggaccggga agaggccgtc ggggaaaagc ctttccgggt
     3061 gggccaggag gctggcccgc tcccaccccg tgatgtggcg gatcaggagg tcggcctcga
     3121 gccggggact atcgatcccg gcctcggcca gtctcgcggc ggcttctttt cgtacttccc
     3181 gaaggttcaa ggctcatcct tccatggatc tcagcttctc cgcctggtct gccatgacca
     3241 gggcgcttat catttcctgg aggtcccctt cgagcatgct gtccagccgg tggagggtca
     3301 agccgatgcg atgatcggtc acccgattct gggggaagtt gtaggtgcgg atcctctcgg
     3361 acctttcacc cgtcccgacc tgccccttcc tttcggcggc catcttctcc tgctgttgcc
     3421 tgagttcgag gtcgtaaagc ttggccctca ggaaattcat ggccctgatg cggttcttga
     3481 tctgcgacct ttcgtcctgg cacgtaacga caaggccggt gggaaggtgg gttatcctga
     3541 cggcggagtc ggtcatgttc acgtgctgtc cccccgcccc gctggcccga taggtgtcga
     3601 tcttgaggtc ttcctgcctg acctcgacgt cgacatcctc ggcctcggga agcaccgcca
     3661 ccgtcgcggt ggatgtatgg atcctcccgc tcgattcggt cacggggacc ctctggaccc
     3721 tgtggacgcc gctttc
//
LOCUS       DAOSRG010000099         3725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22224_length_3725_cov_8.035695, whole genome shotgun sequence.
ACCESSION   DAOSRG010000099 DAOSRG010000000
VERSION     DAOSRG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22224_length_3725_cov_8.035695"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLM30_04370"
     CDS             complement(<1..934)
                     /locus_tag="PLM30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOR54435.1"
                     /translation="MTAAVPEDLRMTPMLKQYAQWKKRYPDCLLFFRMGDFYEMFFED
                     ARVASSLLDITLTARDPGKKIPMAGVPFHSVDSYLERLVASGHKVAICEQVSEPDGRT
                     LVDRQVVRVVTPGTFLPSDSALEGRLACVDVLPDGVAVALLNFATGRLLAGTLGHAPA
                     RSELAGFAPSEVLCPSGQEKTAAALAGDRGGCVVTPRSRDDFRLPGAVRRICREWGVA
                     SLEGFGLSDLDPAAGCAAALLAFVEETQFRSARGAVEIRPLLEKTSMLIDPAAAWNLE
                     VADPPGSSLLDTLNRCRTAMGRRKMRDWLLHPLID"
     gene            complement(931..1350)
                     /gene="ruvX"
                     /locus_tag="PLM30_04375"
     CDS             complement(931..1350)
                     /gene="ruvX"
                     /locus_tag="PLM30_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOR54436.1"
                     /translation="MKRTLGLDIGRVRIGVAVSDPLGAFAQGIDVLDAGKDWMSLVRD
                     IVRERGVGAVVVGVPVNTDGSRGPEAERVLGIIERLREEIGDESVEVVPWDERFSTAQ
                     AERALLEGDLSRRRRRSVIDKVAASLILQSYLESRRP"
     gene            complement(1347..>3725)
                     /gene="alaS"
                     /locus_tag="PLM30_04380"
     CDS             complement(1347..>3725)
                     /gene="alaS"
                     /locus_tag="PLM30_04380"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR54437.1"
                     /translation="GNFSFGDYFKSGAAAFAWEFLTVEAGLDPDRLYPTIYKDDEEAF
                     SIWNGEIGVPQERIYRMGEEDNFWSVGPVGPCGPCSELIYDQGPSFSCGRPDCGVGCE
                     CDRYLEVWNLVFMQYNRLEDGTLEPLPKQNIDTGMGLERLSSVVQQVEGDFQTDLFKP
                     LIDKSCEICGTRYGDGPSSDRAARVIADHFRATAFMIADGVLPSNEGRGYVLRRILRR
                     AVRFGRLFGVDRPFLMELYPVLIDVMGDPYGELIEQRPLIGQVVELEEDRFGKTLEMG
                     LHLLESEIGSLAPDEGDTLSGSVAFELYDTFGFPYELTEEVCQEKGVSVDREGFEEEM
                     EKQRERARAGAKTASRGIGKSLFDEIREEQGATAFCGYVSERANTTITAVIVDDARVA
                     SAPEGSEALLVLGETPFYGEGGGQVGDRGTISGESFLADVTDTIKTSGDLVAHRVVVT
                     RGEAAVGLPVVAEVDGPRRSAIRAHHTSTHLLHEALTRVLGRHVTQAGSLVNEEMLRF
                     DFTHMKPLSGEEIGEVEVMVNRQVLLNTPLEVILTDMESAKRLGAKALFNEKYGSEVR
                     VVRVPGFSAELCGGTHVDATGDIGSFKIIREEGIGSGVRRITALAGLPAFLHHQRLSG
                     QVEALERIFSVPAEELEKRAGEVIRENRHLSGQLEKQKSRQALSRVEELLRGREEAGG
                     VSVVTGVLEGSGPEALREVGDRVRQRLSPVVVVLAVGEGDRGHLVAMADKKAVALGVD
                     CGILVRRIAASLGGGGGGRADMAQAGFRDVAGLPAALEGVRELVSDMTGEGV"
BASE COUNT          586 a         1336 c         1159 g          644 t
ORIGIN      
        1 cgtcgatgag ggggtgcagg agccaatccc tcatcttcct gcggcccatg gcggtcctgc
       61 accggttgag cgtgtcgagg agggagcttc ccggcgggtc ggctacctcc aggttccagg
      121 ccgcggcggg gtcgatgagc atggaggtct tttcaaggag gggcctgatc tcgacggcgc
      181 cccgggcgga cctgaactgg gtctcctcga cgaaggcgag caaggcggcg gcacatccgg
      241 cggcggggtc aaggtcggaa agcccgaaac cctcgaggga ggcgacgccc cattcgcggc
      301 atatcctcct gacggcgccg gggagcctga aatcatccct gcttcgggga gtgacgacac
      361 agccccccct gtcaccggcc agggccgcgg cggtcttctc ctgcccggaa ggacagagca
      421 cctccgacgg ggcgaacccc gccagttcgg acctcgccgg ggcgtggccc agggtcccgg
      481 ccagcagcct gcccgtggcg aagttcaaaa gggccacggc cacgccgtcg ggaagcacgt
      541 cgacgcaggc cagcctcccc tccagggctg aatcgctcgg gaggaaggtc ccgggggtta
      601 ccaccctgac gacctgccgg tccacgaggg tcctcccgtc cggttcggag acctgttcgc
      661 agatggccac cttgtgtccc gaggccacga gcctctcgag gtagctgtcg acggagtgga
      721 agggcacccc cgccatgggt atcttcttac ccgggtccct cgcggtgagg gttatgtcga
      781 gcaaggatga ggccacccgg gcgtcctcga agaacatctc gtagaagtcg cccatcctga
      841 agaagaggag gcagtcgggg tacctcttct tccactgggc gtactgcttc agcatgggcg
      901 tcatcctgag gtcttcgggc accgcggcgg tcatggtctc ctgctctcca ggtaggattg
      961 caggatcagc gaagcggcca ccttgtcaat gacgctcctc ctcctgcgcc tcgaaaggtc
     1021 cccttccagg agggccctct ccgcctgggc cgtggagaac ctctcatccc aggggacgac
     1081 ctcgacgctc tcatcgccta tctcctcccg gagcctctcg atgatcccca ggacgcgctc
     1141 cgcctcgggg cccctcgagc cgtcggtgtt gacggggacg cccacgacga cggccccgac
     1201 cccccgctcc ctcacaatgt ctcttaccag ggacatccaa tccttcccgg cgtcgagcac
     1261 atcgatcccc tgggcgaaag ctcccagggg atcgctcacg gccacgccta tcctcaccct
     1321 gccgatatcg aggcccaggg tccttttcaa accccttccc ccgtcatgtc ggacaccaat
     1381 tcacggacgc cctcgagcgc cgcgggcagg ccggccacgt ccctgaaccc ggcctgggcc
     1441 atatcggccc tgccgccgcc gccgccgccc agggaggccg cgatcctcct caccaggatg
     1501 ccgcagtcga cccccagggc cacggccttt ttatcggcca tggccaccag gtggccccgg
     1561 tcgccttctc ccaccgccag gacgaccacc accggcgaca gcctctgcct gaccctgtcc
     1621 cccacttctc tcagtgcctc ggggccggaa ccctccagga cgcccgtcac gacggagacg
     1681 ccccccgctt cctcgcggcc tcggaggagt tcctccaccc gtgaaagggc ctggcggctc
     1741 ttctgcttct ccagttggcc cgaaaggtgc cggttctccc ggatcacctc accggccctc
     1801 ttctccagtt cctccgcggg gacggaaaag atcctctcca gggcctcgac ctgccccgaa
     1861 agcctctggt ggtgcaggaa agcggggagg cccgccaggg cggtgatcct ccgcaccccc
     1921 gaaccgatgc cctcctcgcg gatgatcttg aaggagccta tgtccccggt ggcgtccacg
     1981 tgggtgccgc cgcagagctc agcgctgaag ccggggacgc ggaccaccct cacctcggag
     2041 ccgtacttct cgttgaagag ggccttggcg ccgagccttt tcgcgctctc catgtccgtc
     2101 agtatcacct cgaggggggt gttgaggagg acctggcggt tcaccatcac ctccacctcg
     2161 ccgatctcct ccccggagag gggcttcatg tgggtgaagt cgaaacggag catctcctcg
     2221 ttgacaaggg acccggcctg ggtgacatgc cgccccagga ccctcgtcag ggcctcgtgg
     2281 aggaggtggg tggaggtgtg gtgggccctg atggcgctcc gccgcgggcc gtccacctcg
     2341 gcgacgacgg gcaggccgac ggcggcctct cccctggtga cgacgaccct gtgggccacc
     2401 aggtcgccgg aggtcttgat ggtatcggtg acatcggcca ggaaggactc cccgcttatg
     2461 gtgcccctgt cgccgacctg gccgcccccc tcaccgtaga agggggtctc ccccaggacc
     2521 aggagggctt cggagccctc gggggccgac gccaccctgg cgtcatcgac gatgacggcg
     2581 gtgatcgtgg tgttggccct ttcggaaacg taaccgcaga aggccgtggc tccctgctcc
     2641 tccctgatct cgtcgaaaag gctcttgccg atgcctcggg acgcggtctt ggccccggct
     2701 ctcgcccttt ccctctgttt ttccatctcc tcctcgaagc cttcacggtc cacggagaca
     2761 cccttctcct ggcagacctc ctcggtcagt tcgtagggga agccgaaggt gtcgtagagt
     2821 tcaaaggcca cgctcccgga gagggtgtcc ccctcgtccg gggccaggga acctatctcc
     2881 gactcgagca ggtggagtcc catctccagg gtcttcccga accggtcctc ctccagttcg
     2941 accacctggc ctatgagggg ccgctgttct atgagctccc cgtagggatc gcccatcacg
     3001 tcgatcagca cggggtagag ctccatcagg aagggcctgt cgaccccgaa gagcctcccg
     3061 aagcgcacgg ccctcctcag tatcctcctg aggacgtagc cccgcccctc gttcgacggg
     3121 aggaccccgt cggctatcat gaaggccgtg gccctgaagt ggtccgcgat gaccctggcg
     3181 gccctgtcgc tcgacgggcc gtcaccgtac cgggtgccgc agatctcgca ggacttgtct
     3241 atcagcggct tgaagaggtc cgtctggaag tccccctcca cctgctggac caccgacgac
     3301 aggcgctcca gccccatgcc ggtgtcgatg ttctgcttgg gcagcggctc cagggtgccg
     3361 tcctccaacc ggttgtactg catgaagacg aggttccaca cctccaggta gcgatcgcat
     3421 tcgcagccca cgccgcagtc gggcctcccg caggagaagg agggaccctg gtcgtagatc
     3481 agttccgagc aggggccgca ggggcccacg ggccccacgg accagaagtt gtcctcctcg
     3541 cccatccggt agatccgttc ctgggggacg cctatctcac cgttccagat ggagaaagcc
     3601 tcctcgtcgt ccttgtagat ggtcgggtag agcctgtccg ggtccagtcc cgcctccacg
     3661 gtgaggaact cccaggcgaa ggcggcggcg ccgctcttga agtaatcgcc gaagctgaag
     3721 ttgcc
//
LOCUS       DAOSRG010000100         3691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22504_length_3691_cov_7.173817, whole genome shotgun sequence.
ACCESSION   DAOSRG010000100 DAOSRG010000000
VERSION     DAOSRG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22504_length_3691_cov_7.173817"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLM30_04385"
     CDS             <1..1379
                     /locus_tag="PLM30_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR54438.1"
                     /translation="VSVSNKHIEVILRKVAPVNRVRVIEEGDSSFVSMELAWLSDIEK
                     EVEQIRAGNERFLQEAVRIMAGKKLGDVAGQGDIEPALSVKGKVLDDGSIRQMLRPGS
                     GVSELTLEDEGDVLRVIVGESSFRRSMTGLVLTEKVELPDGEVIEPGSALSGQNLALL
                     TRGFPPRILVRDVAILEEMKGKTYLAEDVVADGKALLSADRVIDDGAASVIRENNVVS
                     VKVWRSPETVTVSDSIQKMLIDQYWARPLVQAIDSDGNAVIDIPQLIDGSVVRGLVEG
                     ELTAVEIDGEILSRDAILRKVLSDIAYGKVLLEPVKDKKGEVVADAGSEVNSQVLESL
                     LTADPDEMVLRPIFTQSETKRTLQRVTFVRRLREEPAWKPVIHGITKAALATESFLSA
                     ASFQQTAQVLAGAAVRGDVDKLKGLKENVIIGHLIPAGTSFEDCKDIIVREAATAPEG
                     PPPGEVFL"
     gene            1471..1728
                     /locus_tag="PLM30_04390"
     CDS             1471..1728
                     /locus_tag="PLM30_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOR54439.1"
                     /translation="MPLNELAVPNRLVGVRSVRRALQSGNLRKLFLAADARASLVEDL
                     EKMAEEAGIKVEWADSMTILGRACAIGRGAAAAGIAIRKGP"
     gene            1782..2153
                     /gene="rpsL"
                     /locus_tag="PLM30_04395"
     CDS             1782..2153
                     /gene="rpsL"
                     /locus_tag="PLM30_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR54440.1"
                     /translation="MPTINQLIRKGRQEKRGHSKAPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGFEVTSYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DCGGVEGRRKSRSKYGARRPK"
     gene            2193..2663
                     /gene="rpsG"
                     /locus_tag="PLM30_04400"
     CDS             2193..2663
                     /gene="rpsG"
                     /locus_tag="PLM30_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR54441.1"
                     /translation="MPRKGHVRIREVEPDPRFGELDIAKFIAAIMIDGKRSVAEKIMY
                     GALDLASKRLSMEPLEVFNKAMENVRPMVEVRPRRVGGATYQVPVEVQPRRAQALATR
                     WILQYARGRKGIPMVERLARELTDACRGEGNSVKKREDTHRMAEANRAFAHYRW"
     gene            2737..>3691
                     /locus_tag="PLM30_04405"
     CDS             2737..>3691
                     /locus_tag="PLM30_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOR54442.1"
                     /translation="MSSIDLGSIRNIGIAAHIDAGKTTTTERILFFTGRKHKLGEVHE
                     GAATMDWMEQERERGITITSAATTCHWKGFSVNIIDTPGHVDFTVEVERSMRVLDGAV
                     AVFCAVGGVEPQSETVWRQADRYRVPRVAFINKMDRVGADFHQVVAQMKERLGANAVP
                     VQIPIGSEDGFSGVVDLVREKAVIYVEELGVDPRVVDIPAELAEDAALGREALVEAMA
                     EIDEDVMNLFLEGKRVPEDVLRRALRKGTIELAVFPVLCGSAFKNKGVHSLLDAVVDY
                     LPSPKDLPPIKGVHPSTGDPEERYPDPGGPFSALAFKIMVDP"
BASE COUNT          774 a         1033 c         1266 g          618 t
ORIGIN      
        1 gcgtctccgt cagcaacaag catatcgagg tcatcctaag gaaggtcgcg cccgtgaaca
       61 gggtgagggt gatcgaagag ggggacagct ccttcgtctc catggagctg gcgtggctca
      121 gcgatatcga gaaagaagtc gagcagataa gagctggcaa cgagcgcttc ctccaggagg
      181 ccgtgaggat catggccggc aaaaaactcg gtgacgtcgc ggggcagggc gatatagaac
      241 ccgccctgag cgtcaagggc aaggtgctcg atgatggctc gatccggcag atgctccgcc
      301 ccggttcggg cgtctccgag ctgaccctcg aggatgaagg cgatgttctt cgcgtgatcg
      361 tcggggaatc ctccttcagg aggagcatga cgggactggt gctgaccgaa aaggtcgaac
      421 tgcccgatgg cgaggttatc gagccgggga gcgccctctc gggccagaac ctggcgttgc
      481 tgacccgggg cttcccgccc aggatcctgg tgagggacgt ggctatcctg gaggagatga
      541 agggcaagac ctacctcgcc gaggatgtcg tcgccgacgg caaggcgctc ctttccgccg
      601 acagggtcat cgatgacggg gccgcctcgg tcataaggga gaacaacgtg gtgtccgtta
      661 aggtctggcg gtccccggag acggtcaccg tatccgactc gatccagaag atgctcattg
      721 accagtactg ggcgaggccc ctcgtgcagg ccatcgactc cgacggtaac gccgtcatcg
      781 acatacccca gcttatcgac gggagcgtcg tgcgaggcct cgtggagggt gaactgacgg
      841 ccgtcgagat cgacggcgag atactgtccc gggacgccat cctgaggaag gtgctctccg
      901 atatcgccta cgggaaggtg ctgctggagc cggtgaagga caaaaagggc gaggtcgtgg
      961 ccgatgccgg gagtgaggtt aacagccagg tcctggagag cctgctaacg gccgaccccg
     1021 atgagatggt cctgaggccg atcttcaccc aatccgagac aaaacgcacc cttcagcggg
     1081 tgacctttgt ccgcaggctc agggaggaac cagcctggaa gcccgtcatt cacggcatca
     1141 ccaaggcggc ccttgccacg gagagtttcc tcagcgcggc ctccttccag cagacggccc
     1201 aggtccttgc cggtgcagcc gtgaggggcg acgtggacaa actgaagggc ctcaaggaga
     1261 acgtgatcat aggacacctg atccccgcgg ggacatcctt cgaggattgc aaggatataa
     1321 ttgtccgtga ggcggcgacg gcgcccgagg ggccaccccc cggggaggtt ttcctgtaac
     1381 cctggaagag gcggggaaaa gatctttctt gacagtgacc ggtgccgctg atattatttc
     1441 ccggtgtcct tttcaagggg ggggtcccgt gtgcccttga acgagctggc tgtgcccaac
     1501 aggctggtgg gggtaaggtc cgtgaggaga gcgctccagt ccgggaattt gaggaaactc
     1561 ttcctcgcgg ccgatgcgag ggcatccctg gtggaggacc tggagaagat ggctgaagag
     1621 gccggcatca aggtcgaatg ggctgacagc atgaccatcc ttgggcgcgc ttgcgccatc
     1681 ggccgcggag cggctgcggc ggggatcgcg atacggaagg gtccctgagg tcaccgaggt
     1741 ggagggcttg aaggagcagt gaatgagaag gagggggttt tttgccgacc attaaccaac
     1801 tgatacgaaa ggggcgccag gaaaagagag ggcactccaa ggcaccggcc ctgcagggga
     1861 atcctgccag gagaggggtc tgcaccaggg tttacaccgt cacgcccaag aagcctaatt
     1921 ccgccctcag gaaggtcgcg agggtccgcc ttaccaacgg cttcgaggtc acttcctaca
     1981 tacccggggt gggacacaac ctgcaggaac actctgtagt gttggtgagg ggcggcaggg
     2041 tcaaggacct tcccggggtc cgttaccata tagtgagggg tacccttgat tgcggcggcg
     2101 tcgagggaag acggaagagc cgctccaagt acggcgcgag acggcccaag tagggccggc
     2161 agacaggcaa cggcagggag agtgtctagg atatgccaag aaagggacac gtcaggattc
     2221 gcgaggttga gcccgacccc aggttcgggg aactcgatat agccaagttc atagcggcga
     2281 tcatgatcga cggcaagaga agcgtcgccg agaagatcat gtacggagcc cttgacctcg
     2341 cctcgaagag gctgagcatg gagcccctcg aggtgttcaa caaggccatg gagaatgtcc
     2401 gccccatggt cgaggtccgt cccaggaggg tgggcggggc cacctaccag gtccccgtgg
     2461 aagtgcagcc caggagggcc caggccctcg ccacccggtg gatcctccag tacgccaggg
     2521 ggcgaaaggg catccccatg gtagaaaggc tggccaggga gctgaccgat gcttgccgcg
     2581 gtgaggggaa ttccgtcaag aaacgggaag acactcacag gatggccgag gccaacaggg
     2641 cctttgccca ttaccgctgg tagtcagctt aaggctccgc gggcaccggg tcgtgcaagg
     2701 gggacccgcc gcgggaccgg gcacggagaa aagcgaatga gctcgatcga tctcggttca
     2761 atcaggaata tcggcatcgc ggcgcatatt gacgccggca agaccacgac gacggagagg
     2821 atcctgttct tcacgggccg aaagcacaag ctcggggaag tccatgaagg cgcagccacc
     2881 atggactgga tggaacagga gagggagcgg gggatcacta taacctccgc ggccacgacc
     2941 tgccattgga agggttttag tgtcaatatt attgatacgc ccggccacgt ggatttcacc
     3001 gttgaggtcg aaaggtccat gagggtcctc gacggggccg tggcggtctt ctgcgccgtc
     3061 ggcggggtgg aaccccaatc cgagactgtc tggaggcagg ccgaccggta cagggtgccc
     3121 agggtcgcct tcatcaacaa gatggacagg gtcggggcgg atttccacca ggtcgtcgcc
     3181 cagatgaagg agcgcctagg cgctaacgcc gtccccgtgc agatacccat aggttccgag
     3241 gatggcttca gcggtgtcgt cgacctggtc agggaaaagg ccgtcatcta cgtggaggag
     3301 ctcggcgtcg accccagggt agtggatatc ccggccgagc tggctgaaga cgccgccctt
     3361 gggagggaag cccttgtgga ggccatggcg gagatcgacg aggatgtgat gaacctcttc
     3421 ctcgagggca agcgcgttcc cgaagatgtc ctcaggaggg ccctcaggaa ggggaccatc
     3481 gagctggccg tttttcccgt gctctgtggg tcggctttca agaacaaggg tgtccattcc
     3541 ctccttgacg cagtggtgga ctatctcccg agcccgaagg acctgccgcc gatcaagggc
     3601 gtgcacccct cgacgggaga tcccgaggaa aggtaccccg accccggcgg tcccttctcg
     3661 gcgctggcct tcaagataat ggtcgacccc t
//
LOCUS       DAOSRG010000101         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22632_length_3675_cov_10.872099, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000101 DAOSRG010000000
VERSION     DAOSRG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22632_length_3675_cov_10.872099"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLM30_04410"
     CDS             <1..561
                     /locus_tag="PLM30_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR54443.1"
                     /translation="SFTRGARGFPGVTNFTTLPVAAAFFLGMLILMRFLINGNYGRAF
                     KACRDDYIAASLLGFNTSHYRVLSLTISGFYCGIAGALLAGFLTYLQPVMFDMNKSTE
                     LTAVVVFGGLGSLSGSIIGTAVIGMVTEVFRPISQYRMLIYGATMVLIMVVRPEGIMG
                     QYEIGPKLFRSIFSRKPRNEVDGAHG"
     gene            554..1333
                     /locus_tag="PLM30_04415"
     CDS             554..1333
                     /locus_tag="PLM30_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54444.1"
                     /translation="MADPILSLDNLNKHFGGVHAVKDFTFQMERGELAGLIGPNGAGK
                     TTVFNLITGVYSPDSGSIKFKGEEISARKTYEVISRGVARTFQNLRLFPRSTALENVM
                     TARQQHFKYNFFEAVTHAGRWAGNEKKIRETSLELLERVGLLPVAEQAAGTLPYGHQR
                     RLEMARALALQPELLLLDEPAAGMNPEEVQDLNGLIRNIHEDFNLTTLVIEHHMEVIM
                     EICPRIACMNFGEKIAEGDPDYIQKHHEILKAYLGEEVEEC"
     gene            1327..2055
                     /locus_tag="PLM30_04420"
     CDS             1327..2055
                     /locus_tag="PLM30_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54445.1"
                     /translation="MLTKEPLLSVRNLHVSYGAIRAIRGIDLDVYDGEIVCVIGANGA
                     GKSTLMNAIIGNVKREEGTLLLDGKPLAGRSYQAVAQGVSLAPEGRKVFAPLTVHENL
                     MMGAFPIKDRSKIGEDLDWVFTLFPRLKERKDQHAGTLSGGEQQMLSIGRALMSSPRL
                     LLLDEPSLGLAPIIIRDIFKELKRINDEGVTILLVEQNARQALLLSHRGYVMQTGRML
                     MEGLAADLLKNPDVEAAYLGTAKK"
     gene            complement(2103..2540)
                     /locus_tag="PLM30_04425"
     CDS             complement(2103..2540)
                     /locus_tag="PLM30_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR54446.1"
                     /translation="MSRSRKILSVVAISVLVLVAISGIASAQEKIGVIDPQKVLFQHP
                     KFEKAQKDIKAIVEKKQAEAKDAIDKTSDNNEKQRIFEAKRQEAAKEEQRIMEPLFKD
                     IDLAIRTVAKAKGVSIVLDKTQVFFGGADITEDVIQEIKKKNA"
     gene            2659..2880
                     /locus_tag="PLM30_04430"
     CDS             2659..2880
                     /locus_tag="PLM30_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOR54447.1"
                     /translation="MDCIATFEDAQMAVLFDKMCRNMGFFCRIVPVPKGIVASCGLAC
                     RIKEEDAPMIRGLAMEHSIEVLDYHFPGQ"
     gene            complement(2904..3581)
                     /locus_tag="PLM30_04435"
     CDS             complement(2904..3581)
                     /locus_tag="PLM30_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOR54448.1"
                     /translation="MLTRFDWERAVDLVLHFLWPLSCPVCGKLGSALCRECLLDLVGG
                     EPGKISCSECLGPYPCPVHPGAFPVKSLTPFGGLSRELVHRLKYGSQKCLGKPIGDLM
                     GRRFQAPGRVILVPVPLHRGSGRAFNQSMEIARGISRAWGCTAAEKLAWKTRRSSQVG
                     LSPGLRRKLPQDSITWKGAIGRGLPVILVDDVCTTGATLRVAAGAVMTAGGRVEGAVV
                     WALAGAS"
BASE COUNT          727 a         1166 c         1079 g          703 t
ORIGIN      
        1 tccttcacca ggggggccag ggggttcccc ggggtgacga acttcaccac cctccccgtg
       61 gcggcggcct ttttcctggg catgctcatc ctcatgagat ttttaataaa cggcaactac
      121 gggagggctt tcaaggcctg ccgggacgac tacatagcgg catcgcttct gggcttcaat
      181 acttcccatt accgggtcct gagcctcacc atatcgggct tctactgcgg catcgccggc
      241 gcgctgctgg ccggtttcct gacctacctt cagccggtca tgttcgacat gaacaagtcc
      301 acggagctca ccgccgtggt ggtcttcggg ggactcggct ccctgagcgg gtccatcatc
      361 gggaccgccg tgatcggcat ggtgacggaa gtgttcaggc ccatctcgca ataccggatg
      421 ctcatttacg gcgccacgat ggtcctgatc atggtcgtca ggccggaggg gatcatgggg
      481 cagtacgaga tcggaccgaa gctgttccgt tccattttct caagaaaacc gaggaacgag
      541 gtggacggag cccatggctg atcccatcct ctccctcgat aacctgaaca agcacttcgg
      601 cggcgtccac gccgtcaagg atttcacctt ccagatggag cggggtgaac tggccgggct
      661 gatcgggccc aacggggccg ggaagaccac ggtgttcaac ctcatcaccg gcgtctattc
      721 ccccgactcg gggagcatca agttcaaggg cgaggagatc tccgccagga agacctacga
      781 ggtcatctcg agaggtgtgg ctaggacctt ccagaacctc cgccttttcc cgcgctccac
      841 ggcgctggag aatgtcatga ccgcccgcca gcagcacttc aagtacaact tcttcgaagc
      901 ggtcacccac gccggccgat gggccggcaa cgagaaaaag atcagggaga cgagcctgga
      961 actcctcgaa agggtggggc tcctccccgt cgcagaacag gccgcgggga ccctccccta
     1021 cggccaccag aggcggctgg agatggccag ggcgctggcc ctgcagcccg aactcctcct
     1081 cctggacgaa cccgccgcgg ggatgaaccc cgaggaggtc caggacctga acggcctcat
     1141 aaggaacatc cacgaggatt tcaacctgac gacgctggtg atagaacacc acatggaagt
     1201 catcatggag atctgccccc gtatcgcctg catgaacttc ggcgagaaga tcgccgaggg
     1261 tgacccggac tatatacaga aacaccacga gatcctgaag gcctatctcg gggaggaggt
     1321 cgaggaatgt tgactaaaga gcctctcctg agcgtccgga accttcacgt cagctacggg
     1381 gcgatccgcg ccatccgggg gatcgacctg gacgtctacg acggcgagat cgtctgcgtc
     1441 atcggggcca acggggcggg caaatcaacc ctcatgaacg ccattatcgg caacgtaaaa
     1501 cgggaggagg gcaccctcct gctcgacggg aagcccctgg cgggccgtag ttaccaggcg
     1561 gtggcccagg gcgtctccct cgcccccgag gggagaaagg tcttcgcccc cctgaccgtc
     1621 catgagaacc tcatgatggg ggccttcccc atcaaggaca ggagcaagat cggggaggac
     1681 ctggattggg tcttcacgct cttccccagg ctgaaggaga gaaaggacca gcacgccggg
     1741 accctttctg ggggggagca gcagatgctc tccatcggaa gggcgctcat gtcgagcccc
     1801 aggctgctcc tgctggacga accttccctg ggcctggcac ctatcatcat cagggacata
     1861 ttcaaggagc tcaagaggat aaacgacgag ggcgtgacca tcctgctggt ggagcagaac
     1921 gcccgccagg ccctgctgct ctctcacagg ggttatgtca tgcagacggg gaggatgctc
     1981 atggaaggcc tcgccgcgga cctgctcaaa aaccccgacg tggaggcggc ttacctgggc
     2041 acagccaaaa aataaggtcc gggccccacg ggcccggacc ttttctttac ggggcggcga
     2101 tcctaggcgt ttttcttctt gatctcctgg atgacatcct cggtgatgtc cgctcccccg
     2161 aagaacacct gcgtcttgtc gagaactatt gacacaccct tcgccttggc aacggtcctg
     2221 atagcgaggt cgatatcctt gaagaggggt tccatgatcc tctgctcttc cttggccgct
     2281 tcctgcctct tggcctcgaa gatcctctgc ttttcgttgt tgtcgcttgt cttgtcgatg
     2341 gcatccttgg cctcggcctg cttcttctca acaatggcct tgatatcctt ctgggctttc
     2401 tcgaacttcg ggtgctggaa aagcaccttt tgggggtcga taacccctat cttctcctgg
     2461 gcggaggcga tgcctgaaat tgctaccaga accaggacac ttattgccac aaccgaaagg
     2521 atcttccgcg agcgagacat ttaagaacct cctttaaatt cactcgatag gtcaatattc
     2581 tatcctcaca aacccggaac gtccagttat aattacctag tcgcgatcgc acgattacga
     2641 tgggacggag gccgcgtgat ggactgcatc gccaccttcg aggacgccca aatggcggtc
     2701 ctcttcgaca aaatgtgccg gaacatgggt tttttctgca ggatcgtgcc ggtccccaag
     2761 ggcatcgtcg ccagttgcgg cttggcctgc cgcatcaagg aggaagatgc ccccatgata
     2821 cggggacttg cgatggagca ctccatagaa gtcctggatt atcacttccc cggtcaatga
     2881 attcccccct cgccgactcc tgttcacgag gccccggcga gagcccagac gacggctcct
     2941 tcgaccctgc ctccggcagt catgaccgcc cccgccgcaa ccctcagcgt cgccccggtg
     3001 gtacagacgt cgtcgaccag gatcacgggc agccccctgc cgatcgcacc cttccaggtg
     3061 atcgagtcct gggggagttt tctgcggagt cccggcgaga gccccacctg ggaggaccgc
     3121 ctggtcttcc aggccagttt ttcggccgcg gtgcaacccc acgcccggga tatcccccgg
     3181 gcgatctcca tggactggtt gaaggctctc ccgctcccgc ggtggagggg gaccggtacc
     3241 aggatgaccc tccccggggc ttggaacctc ctacccatga gatccccgat gggcttgccc
     3301 aggcacttct gggaaccgta cttcagccgg tggaccagtt ccctcgagag tcccccgaag
     3361 ggcgtgaggc ttttcacggg gaaggcgccg gggtgaaccg gacagggata gggccccagg
     3421 cattcggagc aggagatctt ccccggctca ccgccgacga gatccagaag gcactcacgg
     3481 cacagggcgg agcccagctt tccgcacacc gggcaggaca ggggccacag gaaatgaagc
     3541 acaaggtcca cggccctttc ccagtcaaag cgcgtcaaca tgccgctctc cgggggcgca
     3601 tcgctgcaga gggcttcagc agctcttttc ggcggccctt ttcagggttt cgaccctgtc
     3661 tatccgttcc caggc
//
LOCUS       DAOSRG010000102         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22733_length_3663_cov_9.858647, whole genome shotgun sequence.
ACCESSION   DAOSRG010000102 DAOSRG010000000
VERSION     DAOSRG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22733_length_3663_cov_9.858647"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /gene="rpsC"
                     /locus_tag="PLM30_04440"
     CDS             complement(<1..179)
                     /gene="rpsC"
                     /locus_tag="PLM30_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521530.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR54449.1"
                     /translation="MGQKVHPVGYRLGIVRDWESRWYSGRKDYAEKLHEDLKLREWIL
                     KRWSHAGISKIEIER"
     gene            complement(193..525)
                     /gene="rplV"
                     /locus_tag="PLM30_04445"
     CDS             complement(193..525)
                     /gene="rplV"
                     /locus_tag="PLM30_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR54450.1"
                     /translation="MEAKAIARQVRISPTKSRQVLTLIRGKNAGQALETLRFTPKKAA
                     RVVEKVLKSAIANAEHNYGMDMDRLVVVKAYADQGPSMKRWQPVSMGRVHPYKHRTSH
                     ITVVVAER"
     gene            complement(537..818)
                     /gene="rpsS"
                     /locus_tag="PLM30_04450"
     CDS             complement(537..818)
                     /gene="rpsS"
                     /locus_tag="PLM30_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR54451.1"
                     /translation="MARSTKKGPFVEQKLLHKVEAMNEEGKKRVIKTWSRRSTIVPDM
                     IGHTIAVHNGRTHIPIFISENMVGHKLGEFAPTRKFGGHAGQERSTRVR"
     gene            complement(838..1665)
                     /gene="rplB"
                     /locus_tag="PLM30_04455"
     CDS             complement(838..1665)
                     /gene="rplB"
                     /locus_tag="PLM30_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR54452.1"
                     /translation="MGIKKYKSYTPGRRFMTTSSFEEVTRTEPERSLVEALKKSGGRN
                     NLGRITMRHRGGGSKRLYRIIDFKRDKIGVPGKVASIEYDPNRSARIALIHYVDGEKR
                     YILAPSGLGIGSMITAGPGSDIRPGNAMKLRDIPEGTFIHNIELEPGRGGVLVRSAGT
                     SAQLMAKEGKKAFIRLPSGELRLALLECMATVGQLGNEDHENLTRGKAGKSRWLGRRP
                     RVRGMVMNPVDHPMGGGEGKSKSHKHPVSPWGTPAKGYRTRKRKPSDKFIVRRRYEK"
     gene            complement(1684..1980)
                     /gene="rplW"
                     /locus_tag="PLM30_04460"
     CDS             complement(1684..1980)
                     /gene="rplW"
                     /locus_tag="PLM30_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR54453.1"
                     /translation="MKSLPQDIILRPIITEKSSRLMEHNKYTFEVHPKANKIEVRNAV
                     SSIFKVKVVKVSTLYVRPKPKRMGAFLGKSRAWKKAIVTVAAGERIEFFEGAGA"
     gene            complement(1977..2603)
                     /gene="rplD"
                     /locus_tag="PLM30_04465"
     CDS             complement(1977..2603)
                     /gene="rplD"
                     /locus_tag="PLM30_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR54454.1"
                     /translation="MPTVKVINFKGELIGEENLSDVVFAAPVHVPAMHQVVVAQQANA
                     RQGTHSTRSRGEVQGGGRKPWRQKHTGRARHGSRRSPLWVGGGVTHGPRPRDYHQKVN
                     RKVRRIALKSALSLKVLEGNLVLLDRLEIEKPDTKGMASFLTEIDAGKKVLLMVGESS
                     GAIYKSASNIPGARVLHVDSINVYDILDNNRMILTVDAARKIEEVYGA"
     gene            complement(2619..3260)
                     /gene="rplC"
                     /locus_tag="PLM30_04470"
     CDS             complement(2619..3260)
                     /gene="rplC"
                     /locus_tag="PLM30_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR54455.1"
                     /translation="MVLMGILGRKLGMTQIFDEQGRSIPVTVVEAGPCNVVAVKSLDA
                     DGYTAVSLSFGDTKPSKLNRPRRGVFEKRGLEPKRWIREFRVGDTDGFEVGGVVSVEL
                     FEAGDVVDVTGRSKGKGFAGVIKRHHFGGGPASHGASKFHREPGSGGASSYPGHIFKG
                     KTMPGRMGGERVTVKNLSVAGVDPENNLLLIRGAVPGPRNGLLMIRKRAGAPR"
     gene            complement(3288..3593)
                     /gene="rpsJ"
                     /locus_tag="PLM30_04475"
     CDS             complement(3288..3593)
                     /gene="rpsJ"
                     /locus_tag="PLM30_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR54456.1"
                     /translation="MAKKIRIKLKAFDHRALDSSAFQIAETAQRTGAKVSGPIPLPTE
                     VNKFTVLKSPHKDKDAREQFEMRTHKRLIDIIDPTQKTMESLMQLNLPSGVDIQIKL"
BASE COUNT          591 a         1200 c         1012 g          860 t
ORIGIN      
        1 atccgttcga tctctatctt cgagatgccg gcatgggacc atctttttaa gatccattcc
       61 ctgagcttga ggtcttcatg cagtttctca gcgtaatcct tgcggcccga gtaccatctc
      121 gattcccaat cgcgcactat tccaagccga tacccaacag ggtgtacttt ctggcccaac
      181 ggtcaaccct ccctagcgct cggcgaccac aacggtgata tggctggtgc gatgcttgta
      241 aggatgcacc ctgcccatcg agactggttg ccagcgcttc atagatggac cctggtcggc
      301 gtaggccttc accacaacca gtcgatccat atccattcca tagttatgct cggcattggc
      361 gatggcgctc ttgagcacct tctcgaccac ccgggcggcc ttcttggggg tgaagcggag
      421 cgtctcaagg gcctggccgg cgttcttccc ccttatgaga gtaaggacct gcctcgattt
      481 ggtcggcgag atccgcacct gcctcgcaat ggcttttgct tccatgaaaa gccctcctat
      541 cgcacgcggg tggaacgttc ctgccccgcg tgccccccga acttgcgcgt gggggcgaac
      601 tccccaagct tatgacccac catattctcg cttatgaaga tcgggatatg ggtcctgccg
      661 ttgtgcacgg cgatggtgtg cccgatcata tccggcacaa tggtggaacg cctggaccac
      721 gtcttgatga ctcttttctt tccttcctcg ttcatggcct ccaccttgtg gagaagcttc
      781 tgttcgacaa agggcccctt tttcgttgaa cgagccattg tatgtccctc cctgccgcta
      841 cttctcgtaa cgtcgccgta cgatgaactt gtccgacggc ttgcgtttac gggttctgta
      901 ccccttggcg ggcgttcccc agggggacac cgggtgcttg tgggacttgc tcttcccttc
      961 cccgccgccc atggggtgat ccacggggtt catgaccatg cccctgacgc gggggcgtct
     1021 acccagccat cggctcttgc ctgccttgcc ccgggtgagg ttctcgtggt cctcgttgcc
     1081 caactgtcca acggtggcca tgcactcaag aagggcgaga cggagttccc cgctcggtag
     1141 ccttatgaaa gcctttttgc cctccttggc catgagctgc gccgatgtcc ccgccgaccg
     1201 gaccagtaca ccaccccgac ccggctcgag ttcgatgttg tggataaagg taccctcggg
     1261 tatgtccctc aacttcatgg cgttgccggg gcggatatcc gacccggggc ccgccgtgat
     1321 catgctcccg atcccgaggc ccgagggggc gaggatgtac cttttctcgc cgtcgacgta
     1381 atggatcagg gctatcctcg cggagcggtt gggatcgtac tcgatggagg ccaccttccc
     1441 ggggacaccg atcttgtcgc gcttgaagtc gatgatccga tagaggcgct tggaccctcc
     1501 tccgcggtgc ctcatggtga tcctgccgag gttgttgcgg cccccggact ttttcagcgc
     1561 ctctaccaac gacctctcgg gctcggtcct ggtgacctcc tcgaaggagg aggtcgtcat
     1621 gaacctgcgg cccggggtat aggatttgta ttttttgata cccatcttat taaacctccc
     1681 cgcctaggcg ccggcgccct cgaagaattc gatgcgctcg ccggcagcga cggtcacgat
     1741 agcctttttc cacgccctgg atttgcccag gaaggctccc atcctcttcg gtttcggcct
     1801 cacgtacagg gtgctcacct tgaccacctt gaccttgaag atggaggaca cggcgttcct
     1861 gacctcgatc ttgttggcct tggggtgcac ctcaaaggtg tacttgttat gctccatcag
     1921 ccggctgctc ttttccgtga tgatgggcct caggatgata tcctgcggca atgatttcat
     1981 gccccgtaca cctcctcgat cttcctggca gcgtccaccg tcaggatcat cctgttatta
     2041 tccaggatgt cgtaaacgtt gatgctgtcc acgtggagaa ccctcgcccc ggggatgtta
     2101 gaggccgact tgtagatcgc gccgctgctc tcacccacca tgagcaagac ctttttcccg
     2161 gcgtcgatct ccgtcaggaa ggaggccatg cccttcgtgt cgggcttttc tatctcaagg
     2221 cggtcgagca gtacgaggtt tccctcgagg accttgagag agagcgcgct cttcagggcg
     2281 atcctcctga ccttcctgtt gaccttctgg tgatagtccc tgggcctggg accgtgggtg
     2341 accccgccgc ccacccagag gggggatctc ctgctgccgt gcctggccct cccggtgtgc
     2401 ttctgtctcc agggttttct cccgccgccc tgcacctcgc cccgagagcg ggttgagtgg
     2461 gtgccctgcc tggcgttggc ctgctgggcc accacgacct ggtgcatggc cggaacgtgg
     2521 accggggccg cgaagacgac atcggagagg ttctcctcac cgatgagttc tcccttgaaa
     2581 ttgataactt tcacagtcgg catagcttcg ctgcctcctt accgtggtgc cccggcccgc
     2641 ttgcggatca tcagaagccc gttccttggt cctggaacag cacccctgat cagcaggagg
     2701 ttgttttccg ggtcaacgcc ggcgacggac aggttcttta cggtcacccg ttcgccgccc
     2761 atccttcccg gcattgtctt tcccttgaag atatgaccgg ggtagctgct cgcccccccc
     2821 gaaccgggct ccctgtggaa cttggaagca ccgtgactcg cggggccccc gccgaagtga
     2881 tgtcgcttga taaccccagc gaaccccttc cctttgcttc gccctgtcac atcgaccacg
     2941 tcgccggcct cgaagagttc caccgagacc acgccaccta cctcaaaacc gtcggtgtcg
     3001 cccaccctga actcccggat ccaccttttg ggctcgaggc ccctcttttc gaagacgccc
     3061 ctgcggggcc tgttcaactt ggagggcttg gtgtccccaa aggacaggct caccgccgtg
     3121 tagccgtccg cgtcaaggct cttgacggcg acgacgttgc agggaccggc ctcgacaacc
     3181 gtcaccggga tggaccggcc ctgttcgtcg aagatctggg tcatgcccag cttacgaccg
     3241 agaataccca tcagtaccat ctctctcctt tcagctcatt gctcccccta aagcttgatc
     3301 tggatgtcca ctccggaggg gagattcagc tgcataagcg actccatggt cttctgcgtc
     3361 gggtcgataa tgtcgatgag ccgcttatga gtcctcattt caaactgctc cctcgcgtcc
     3421 ttgtccttgt ggggcgactt gaggaccgtg aacttgttca cttccgtcgg gagggggata
     3481 ggcccggaaa ccttggcccc cgtccgctgc gccgtctccg ctatctggaa ggctgacgag
     3541 tccagcgccc tgtggtcgaa cgccttgagc ttgatcctga tcttcttcgc cactgtgacc
     3601 gcctcccggg atcagctgcc gagtatctcg gtgacgacgc cggcgccgac ggtcctgccg
     3661 cct
//
LOCUS       DAOSRG010000103         3662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22746_length_3662_cov_5.135292, whole genome shotgun sequence.
ACCESSION   DAOSRG010000103 DAOSRG010000000
VERSION     DAOSRG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22746_length_3662_cov_5.135292"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLM30_04480"
     CDS             <1..695
                     /locus_tag="PLM30_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR54457.1"
                     /translation="IFAQLVRFHDREPISRNEFLQMAGRAGRKGLFETGYVTWLKRSP
                     QESRGSDTGKIFRRLMSAQAESASVTLRPDYGKLLKGWRLIPDEAEYIASFSLPPGDR
                     RVVEEELQRGLKYIDRAVREMVDLDWRERFREILADIWYGEMEIGENLEMAHLFLETG
                     KADALTAAQVINLFEKSYLKSLLKVKRFSNHLPAQYEITGMQYLDRTVDEIDGTIYGF
                     EEKIQEIEVSSA"
     gene            complement(706..1974)
                     /locus_tag="PLM30_04485"
     CDS             complement(706..1974)
                     /locus_tag="PLM30_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54458.1"
                     /translation="MVMTSELFRISQERLEEVNRFLLDPANPEVQALLRVVAKYGTPE
                     EINAKARAARCLSSLMGRLERMGSPYLENLRWLESERDKGAFIPISEYRKKVLGGEPA
                     GRTFRESTSVTLEISALQYFPFLIAEAEQAIDRKEVMPGRYIRVRKMKEQERDQGDTL
                     AVAAAMQIVGASYAETLDTKGTDGSNVHLGGPETITGYFGGIGQPNDHPLAWADELLY
                     YYTNYGINEVLNINPGTVLLGYLLHRLGIDVGFKISVFMGNDNPYAVLWTLMMARMFA
                     REDGSTPLVGFNLSNSVDAPTLLACADIRRRLGLEDKVRIEHHITETYRSIVRQPYNR
                     RADLLEVARVYPNISAKHEGGEPEVEQTLAHPSDILDYFRDKAEVLASGDMDNLKTNY
                     LKKHEAVNNTAEELTKAGLSFIAAQRLHAR"
     gene            complement(2178..3374)
                     /gene="trpB"
                     /locus_tag="PLM30_04490"
     CDS             complement(2178..3374)
                     /gene="trpB"
                     /locus_tag="PLM30_04490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR54459.1"
                     /translation="MKSASKAVEARKGYFGTFGGSYVPEQLQPRLDELEAAFESARGD
                     ELFGKEYARLLKEYVGRPSALTYCANISREYGGGRLFLKREDLNHTGAHKINNTIGQA
                     LLAKRMGKERIIAETGAGMHGTATATVAALMGMKCTVYMGEEDVRRQAPNVARMKVLG
                     AEVVPVTHGQRTLKEAVDAALDAYVAEPESFYMLGSVVGPHPFPTVVRHFQSVIGREA
                     REQFLEREGRLPDNVLACVGGGSNAMGIFSGFLGDDGVKLIGVEPAGRGLDTKDHAAT
                     LSVGKPGIIHGFRSYVLTDGDGEPAPVYSISAGLDYPGVGPEHAFLMESGRASYVSVT
                     DDEAVGAFETLCRLEGIIPALESSHAVAHALKMLPRLTAKETVLVNLSGRGDKDLDTA
                     LPLLGL"
BASE COUNT          672 a         1157 c         1109 g          724 t
ORIGIN      
        1 tgatcttcgc ccaactggtc cggttccacg acagggaacc catctccagg aacgagtttt
       61 tgcagatggc ggggagggcc ggcaggaagg ggcttttcga gacaggctac gtgacatggc
      121 tcaaaaggtc accccaggag tccaggggct ccgacacggg caaaatattc cggcggctca
      181 tgtcggccca ggccgaaagc gcctcggtga ccctgaggcc cgactatgga aaactactga
      241 aagggtggag gctcatcccc gacgaggcgg agtacatagc gtccttctcc cttcccccgg
      301 gcgatagacg cgtcgtggaa gaagaactgc aaagagggct gaagtacatc gaccgggccg
      361 tccgcgagat ggtggacctc gactggaggg agcgtttccg ggagatcctg gccgatatat
      421 ggtacggcga gatggagata ggagagaacc tcgaaatggc ccacctattc ctggagaccg
      481 gcaaggccga cgccctgacg gccgcccagg tgataaacct cttcgagaag agctacctga
      541 agtccctcct caaggtcaag cgcttctcca accaccttcc ggcccagtac gagatcaccg
      601 ggatgcagta cctggaccgg acggtggatg agatcgacgg aacgatctac ggcttcgagg
      661 agaagatcca ggagatcgag gtcagctccg cctgacctgg caggttcagc gggcgtggag
      721 ccgctgggcg gcgatgaacg aaaggcccgc ctttgtaagt tcctcggccg tattgttcac
      781 ggcctcgtgc ttcttgaggt agttggtttt gaggttgtcc atgtcgcccg aggcaaggac
      841 ctccgccttg tcgcggaaat agtccagtat atccgagggg tgggccaggg tctgttcgac
      901 ctcgggttcg cccccctcgt gcttcgccga gatgttcggg tagacgcggg ctacctccag
      961 caggtcggcg cgcctgttgt agggctggcg gacgatgctc ctgtaggtct cggtgatgtg
     1021 gtgctctatc ctgaccttgt cctccaggcc gagcctcctg cggatatcgg cgcaggccag
     1081 cagcgtgggg gcgtccaccg agttcgagag gttgaatccc acgagggggg tcgacccgtc
     1141 ctccctggca aacatcctgg ccatcatcag cgtccagaga accgcgtagg ggttgtcgtt
     1201 gcccatgaat acggagatct tgaagccgac gtcgatgccc agcctgtgga gcaggtaacc
     1261 cagcaggacc gtcccggggt tgatgttgag cacctcgttg attccgtagt tggtgtagta
     1321 gtacagcagt tcgtccgccc acgccaaggg gtggtcgttg ggctggccta tgcccccgaa
     1381 gtagcccgtg atggtctccg gcccgccgag atggacgttg gaaccatcgg tgcccttcgt
     1441 gtcgagggtc tcggcgtaac tggcgccgac gatctgcatg gccgccgcga cggcaagggt
     1501 atcgccctgg tctcgttcct gctccttcat cttgcggacc ctgatgtacc tgccgggcat
     1561 gacctccttc ctgtcgatgg cctgttccgc ctcggcgatc aggaagggga agtactggag
     1621 ggcgcttatc tcgagcgtca ccgacgtgga ctctctgaag gtcctgcccg cgggctcgcc
     1681 gcccaatacc ttctttctgt actccgagat ggggatgaaa gcccccttgt ctctctccga
     1741 ctcgagccac ctgaggttct ccaggtaggg ggaacccatc cgttcgaggc gccccatgag
     1801 gctcgaaagg cacctcgcag ctctcgcctt ggcgttgatc tcctccggcg tgccgtactt
     1861 ggcgacgacc ctcagaaggg cctgcacttc ggggttcgcg gggtcgagca ggaagcggtt
     1921 gacctcttcc agcctttcct ggctgatgcg gaacagttcg ctcgtcataa ccatctcctc
     1981 cggtccggta tttctgttga aaatctcgtt gcctcttcac catgacaaaa cttggcccga
     2041 tgataccaca acccaagagg ttctcaccaa ggactcgagg gaacgaaaaa attcttcgag
     2101 caaggaagca caaaagcgcc ccgctggatc attcaaaaag cccccggagg gctgtccccc
     2161 gggggcgggg ttctactcta cagccccagg agcggtagtg ccgtatcgag gtccttgtcg
     2221 ccccttcccg agaggttaac cagcaccgtc tccttcgccg taagccgggg gagcatcttc
     2281 aatgcatggg ccacggcatg ggaactctcc agggccggga tgataccctc cagccggcag
     2341 agcgtctcga aagcgccgac ggcctcgtcg tcggtgacgg agacgtagga agccctgccg
     2401 ctttccatca ggaaggcgtg ctcggggccc acgccggggt aatccagccc cgccgagatg
     2461 gaataaaccg gcgcgggctc gccgtcgccg tcggtcagga cgtaactcct gaagccgtgg
     2521 atgatgcccg gcttccccac cgagagggtc gcggcgtggt ccttcgtgtc gagcccccgc
     2581 ccggcgggct ccacgccgat gagcttcaca ccgtcgtccc cgaggaagcc ggagaagatc
     2641 cccatggcgt tgctccctcc gcccacgcaa gccaacacat tgtcgggaag cctgccctcc
     2701 ctctcgagga actgctcccg ggcctccctg ccaatgacgc tctggaaatg cctgacgacc
     2761 gtgggaaagg gatggggccc cacgacggaa cccagcatat agaatgattc cggctccgcc
     2821 acgtaggcgt cgagggccgc gtcgacggcc tctttcaagg tcctctggcc gtgggtcacg
     2881 gggaccacct cggcgcccag gaccttcatc cgggccacgt tcggggcctg cctccggaca
     2941 tcctcctcac ccatgtagac ggtgcacttc atccccatca gggccgccac cgtggccgtg
     3001 gccgtcccgt gcatgcccgc cccggtctcg gcgatgatcc tctccttgcc catccgcttc
     3061 gcgagcaggg cctggcctat ggtgttgttg atcttgtgcg ccccggtgtg gttcaggtcc
     3121 tcccgcttga ggaaaagcct tccaccgccg tactccctgg agatgttggc acagtatgtc
     3181 agggcggagg gtctgcccac gtattccttc aaaagcctgg catattcctt gccgaataat
     3241 tcatcacccc tggccgactc gaaggcggcc tccagttcat ccagcctggg ctgcaactgt
     3301 tcgggcacgt aactgccccc gaaggtaccg aaataaccct tccttgcctc gactgccttg
     3361 cttgcgctct tcatttcctc ttcctcctcg gctcacgata tcggggttgt ccgcaaacaa
     3421 aaaggggcgt tcatcctctt atggatacgc cccctcgttc agaataaaaa acgcggggta
     3481 cacggtcgaa accgcgcacc ccgcgtgtag tcacgcgtcg ggtcgcggcg ccttagcgcc
     3541 accaccagtt ggaattgacg gacttcatgg aacctctgct gaactcggct attctcatct
     3601 gcggacactc cctctaggga aaaatgtagc agatttattc gggcgatgca agggggctgg
     3661 gg
//
LOCUS       DAOSRG010000104         3651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_22837_length_3651_cov_8.384594, whole genome shotgun sequence.
ACCESSION   DAOSRG010000104 DAOSRG010000000
VERSION     DAOSRG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22837_length_3651_cov_8.384594"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLM30_04495"
     CDS             <1..251
                     /locus_tag="PLM30_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOR54460.1"
                     /translation="AEEDGEMVVVEVRTRTIGRMLPPEATVGPSKIRKLVRTGTAYMD
                     RIGWEGPWRIDIAAITVGPDGKPALEIFRDITVGIIEP"
     gene            248..>1700
                     /locus_tag="PLM30_04500"
     CDS             248..>1700
                     /locus_tag="PLM30_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOR54461.1"
                     /translation="MNVVNGVTLRGVEALKVEVEVEMTGGLFAISVVGLPDATVKEAR
                     ERVRAALRSLGLSVKGRVAVNLAPADIPKEGALIDLPIAIGIAHAMGEVRVARPTIFM
                     GELALDGRVRRVRGAVPAALLARKLGFPLFIPEGNAREVSLVKGVTAWKVGHIRDLFA
                     HLQGSSQLEPIAPRDEAEENPEPDPDLSDIKGQAAAKRALEIAAAGHHNVLMTGSPGS
                     GKTLLARALKDILPPLSDEEMMEVLLVRSTVGLPLHSSRQRPFRAIHHTSSTISVCGG
                     GTNLRPGEISLAHRGVLFLDEFTEFRRDLLESLRQPLEDGFIPVSRASGSVTYPARVL
                     LVAACNPCPCGFLGDPQEPCRCSPLAVERYRRKMSGPILDRIDLHVTVPRLTPDELVQ
                     FEGQVGENSADVRQRVLRAREVQWRRWGPLGFQCNAELPERALRRQVKIDAEGRRFLK
                     DVAGRVRLSGRGISRVLKVARTIADLAGDEGLTT"
     assembly_gap    1701..1800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1801..2910
                     /gene="dprA"
                     /locus_tag="PLM30_04505"
     CDS             <1801..2910
                     /gene="dprA"
                     /locus_tag="PLM30_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOR54462.1"
                     /translation="EPLKKGMLLLNATGGFDATVLEAADDHGVFWEDIVAGSGTALNA
                     LGVKGAPREALLGLVSSGWCDREVDRCEKKGIEIIFFGEARYPSMLNDLRDPPLVLYA
                     RGKGLPSGEPVAVVGTRKCTSYGSSCAYDLASMLAGAEVTVVSGGAMGIDGAAHRGSI
                     DSGGRTVAVIGTGADIFYPAQHASLFGEIMEEGVLLGEFPLGTPPRPWQFPRRNRLIA
                     GLCSRCVVVEAPQKSGAVLTARLALETGREVWAVPGRITDTVCRGSNALISDGAMPLV
                     DLGEFACLASGSQGELFPLIPRQGLPANSPLTPEEAEVLRILSDHGDMTVDNIATEGK
                     MSAAAVLRVLGVLSAYGLAVPSGPGRWGSRAARGA"
     gene            2940..3185
                     /locus_tag="PLM30_04510"
     CDS             2940..3185
                     /locus_tag="PLM30_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54463.1"
                     /translation="MSVRFIPESESRFLTEDALALAYRACADESLSREVVEDTLTEAV
                     RLGLIGDFPINAGLFESLLYSVHEKTKAPGPKEMPLC"
     gene            3179..>3651
                     /locus_tag="PLM30_04515"
     CDS             3179..>3651
                     /locus_tag="PLM30_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOR54464.1"
                     /translation="MLKDEGSRVKRARAKKAGATPSRGSAKAGAKPAKKRASRAAPEP
                     GRTLVIVESPSKARTLGKILGKGYDIQSSVGHIRDLPKSRLAIDVDNGFEPEYILVRG
                     KGAVVRNLKERASVSGRVLIASDPDREGEAIAWHLSETLGVDPGSPCRVRMYEITP"
BASE COUNT          615 a         1162 c         1220 g          554 t
ORIGIN      
        1 tcgccgaaga ggacggcgag atggtcgtcg tggaggtcag gacgaggacc atcgggagga
       61 tgctcccccc ggaggcgacg gtcggcccct cgaagatccg taaactcgtc aggaccggca
      121 cggcctacat ggaccgcatc ggctgggaag gcccctggag gatagatatc gccgccatca
      181 ccgtcgggcc cgacggaaag cccgcgctgg agatcttccg ggacatcacc gtggggatca
      241 tcgaaccatg aacgtggtca acggcgtgac cctccggggc gtcgaggccc tgaaagtgga
      301 ggtcgaggtg gagatgaccg gggggctttt cgccatatcg gtggtggggc tccccgacgc
      361 cacagtgaag gaggcccgcg agagggtccg ggccgctctg aggtccctgg gcctttccgt
      421 gaagggcagg gtggccgtca acctggcccc ggccgatatc cccaaggagg gggccctgat
      481 agacctcccc atcgccatcg ggatcgccca cgccatgggc gaggtccgcg tggccaggcc
      541 cacgatcttc atgggggaac tggccctcga cggcagggtc cggagggtcc ggggcgccgt
      601 ccctgcggcg ctgttggcca ggaagctcgg ttttcccctt ttcatccccg aggggaacgc
      661 ccgcgaggtc tccctggtca agggcgtcac cgcctggaag gtagggcata tccgcgatct
      721 cttcgcccac ctgcagggct cttcccaact ggagcccatc gcccccaggg acgaagccga
      781 ggaaaacccc gaaccggacc ccgacctctc cgacatcaag gggcaggccg ctgccaaacg
      841 cgccctcgag atagccgccg ccggccacca caacgtcctc atgaccggtt cgcccggctc
      901 ggggaagacc ctcctggcca gggcgctcaa ggatatcctc ccgccccttt ccgacgagga
      961 gatgatggag gtcctcctgg tgaggagcac tgtgggcctc ccactgcaca gctcacgcca
     1021 gcggcccttc cgggccatcc atcacacctc gagcaccata tccgtctgcg gcggcgggac
     1081 gaacctccgg cccggcgaga tcagcctggc ccatcgggga gtccttttcc tcgacgagtt
     1141 caccgagttc aggcgggacc tgctcgaatc cctccgtcag cccctggagg atgggttcat
     1201 ccccgtgagc cgggccagcg gtagcgtgac ctacccggcc agggtgctgc tcgtggccgc
     1261 ctgcaaccct tgcccctgcg gtttcctggg cgacccccag gagccttgcc gctgttcccc
     1321 cctggcggtg gaacggtacc gccgcaagat gtcgggcccc atcctggaca ggatcgacct
     1381 ccacgtcacc gtgccgaggc tgacgccgga cgaactggtg cagtttgagg ggcaggtggg
     1441 ggagaacagc gccgatgtgc gccaaagggt cctcagggcc agggaggtcc agtggcggcg
     1501 gtggggcccc ctgggtttcc agtgcaacgc ggaactgccc gaaagggccc tgaggagaca
     1561 ggtcaagata gacgccgagg gcaggcgctt cctgaaggac gtggccggga gggtccgcct
     1621 ttccggcagg ggcatcagca gggtcctcaa ggtggcgagg accatcgccg acctcgccgg
     1681 cgacgagggg ctcaccacgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 gaaccgttga aaaagggaat gctcctgctg aacgccaccg gcggcttcga cgcaacggtc
     1861 ctcgaggccg ccgacgatca cggggtcttc tgggaggaca tcgtcgccgg ctccggcact
     1921 gccctgaacg ccctcggcgt gaagggcgcc cccagggagg ccttgctggg gctggtttcg
     1981 tcgggatggt gcgaccggga ggtcgaccgc tgcgagaaga agggcatcga gatcatcttt
     2041 ttcggcgagg cccgttaccc ttccatgctg aacgacctcc gcgacccccc tctcgtgctc
     2101 tacgccaggg gaaaggggct gccctccgga gagcctgtgg ccgtggtcgg gaccaggaag
     2161 tgcacttcct acgggtccag ctgcgcctac gaccttgcct cgatgctggc gggcgccgag
     2221 gtcaccgtcg taagcggggg cgcgatgggg atcgacggtg ccgcccacag ggggagcatc
     2281 gactccgggg gaaggaccgt cgccgtgatc gggaccgggg cggatatctt ctacccggcc
     2341 cagcacgcct cactcttcgg ggagatcatg gaagaggggg ttctactggg cgaatttccc
     2401 ctcgggactc ctcccaggcc gtggcaattc ccccggcgga acaggctcat cgccggcctg
     2461 tgctcgaggt gcgtcgtcgt ggaggcgccc caaaagagcg gcgcggtcct caccgcccgt
     2521 ctcgccctcg aaacggggag ggaggtgtgg gccgtgccgg ggcggatcac cgacaccgtc
     2581 tgccgtggtt cgaacgccct catatccgac ggcgctatgc ccctggtgga cctcggggag
     2641 ttcgcctgcc tcgcctcggg gagtcagggt gaactgttcc ctctcatccc gcggcagggc
     2701 cttcccgcca actcccccct cacgcctgag gaggccgaag tgcttcgcat cctgagcgat
     2761 cacggtgaca tgacggttga caacatagcg acggaaggta aaatgagcgc cgccgccgta
     2821 ttgagggtcc ttggggtgct atcggcctac ggcctcgctg tccccagcgg cccgggaagg
     2881 tggggttcga gggccgcaag gggggcctga ggggccccga gggaacagga ggagagagga
     2941 tgtccgtaag attcataccc gaaagcgaaa gccgcttcct caccgaggac gccctggccc
     3001 tggcctatcg tgcctgcgcc gacgagagcc tttccaggga ggtcgtggag gataccctga
     3061 ccgaggcggt ccgcctcggc ctgataggcg actttcccat caacgcgggt ctttttgagt
     3121 cccttttgta ctcggtccac gaaaagacga aggccccagg gccgaaggaa atgccgttgt
     3181 gctgaaggac gaaggatcca gggtgaaaag ggcgagggcg aaaaaggccg gagccactcc
     3241 ctcccggggg tcggcgaagg ccggggctaa gcctgctaaa aaaagggcgt cccgagccgc
     3301 ccctgaaccg gggcggaccc tggtgatcgt cgaatcgccc tccaaggcca ggactctcgg
     3361 caagatcctc ggcaagggtt acgatatcca gtccagcgtg gggcatatcc gcgacctgcc
     3421 caagagccgc ctggccatcg atgtggataa cggcttcgag ccggagtaca tcctggtcag
     3481 gggtaagggg gctgtcgtcc ggaacctgaa ggagagggcc tcggtgagcg gccgggtgct
     3541 catcgcctcc gaccccgaca gggaggggga ggccatcgcc tggcacctct ccgagaccct
     3601 cggcgtcgat cccggctctc cctgcagggt caggatgtac gagataaccc c
//
LOCUS       DAOSRG010000105         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_23054_length_3624_cov_8.902774, whole genome shotgun sequence.
ACCESSION   DAOSRG010000105 DAOSRG010000000
VERSION     DAOSRG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23054_length_3624_cov_8.902774"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..450)
                     /gene="rsfS"
                     /locus_tag="PLM30_04520"
     CDS             complement(109..450)
                     /gene="rsfS"
                     /locus_tag="PLM30_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR54465.1"
                     /translation="MLPDDTVSPLIQALQDKLAEDITIIDVSRESSFADAFILATGNS
                     DVHMKTLLDNLTEAMDRMKRTYRVEGESSPKWILLDAGNVVINIFSREGRDFYRLESI
                     WAGAEMTRVGD"
     gene            complement(484..1758)
                     /locus_tag="PLM30_04525"
     CDS             complement(484..1758)
                     /locus_tag="PLM30_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOR54466.1"
                     /translation="MSKRQVLLLILTAVLSLTAGIGLKAFFFFNPEPETIRERIEHDE
                     ETGRINILLLGLDKVPGEDVNRPDSIAFVSIDIDDKVVRLLSLPRDTRVQIPGRGWQK
                     LNHAYAYGGVELLKTTLVNYLGVPIPYHVIVNLQNFPELVDLVGGVTINVPKRLRYTD
                     RAGGIHIDIPAGEQHMDGKTALGFVRFRHDALGDIGRVERQQVFMMTVFNKIKDPAMI
                     TKIPELIRQGIRLVKTNLSLSQAIQLASYLKDLDPSRVVFRTLPGKAAYISGISYWLG
                     ELGDLSEILTEKPGEPQDNIMEEDRADEPVSATDLDLNLKDTLASIKVSLSILNGSGT
                     AGVGKESATRLQSLGIDVAHIGNAKHYDYKYTNLLFPPGEETEKTARSLGLLFGIAEN
                     LVRKDPSVRNVTMIMGHDYMNIFQRIDLARDQ"
     gene            complement(1766..2404)
                     /gene="nadD"
                     /locus_tag="PLM30_04530"
     CDS             complement(1766..2404)
                     /gene="nadD"
                     /locus_tag="PLM30_04530"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOR54467.1"
                     /translation="MPLRSSEGLRKRKIGVMGGTFDPIHFGHLIAGEEAYFSLGLTEV
                     VFVPTGDPFHKAGRSISSAEHRYVMTMLATLDNPHFRISRIEIDRGTSSHTVDTLREM
                     RHWYPSGSVEFFFITGLDAVLEIPAWKEPLEIPRLCRIVAVSRPGFNPGGLENLPVEI
                     RENLIHLEIPLLAISSTEIRKRVSNGQSIRYLAPWLVEQYIYKNNLYFRNGW"
     gene            complement(2410..>3624)
                     /gene="obgE"
                     /locus_tag="PLM30_04535"
     CDS             complement(2410..>3624)
                     /gene="obgE"
                     /locus_tag="PLM30_04535"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOR54468.1"
                     /translation="HVWIVADDHIQTLADYEYRRKFVAGNGGNGKGRNQTGPDGEDVV
                     VKVPCGTIITEKGTGRFFCDLTVAGDTFLAAKGGRGGRGNARFASSRRRTPRFSEQGE
                     VGEARDLRLNLKILADVGLVGLPNAGKSTLLAAISGSKPQIADYPFTTLSPNLGVMDL
                     DQKKIVVADVPGLIEGAHRNRGLGHAFLKHVERTRLLVYVIDLSREALVTPADQWHTL
                     RAEFRAFNPGLLELPSVVAGNKTDLPSSLPDIKELKRSLEVEKVPFFPISALSGEGIR
                     ALTDHIVSRMPEREEYLREAIFVKIGDEEGRAAQKTEKPEVDVEQHKGEKTFIVRHQE
                     LRRLVGKFDFEQEEALSRFSAILRFYRIEDLLFESGAREGDTVRIGDVEFEFYPEGSS
                     MVDEGTRGRTGV"
BASE COUNT          748 a         1045 c          955 g          876 t
ORIGIN      
        1 taatgcaaca taagatatat tataagacat taccggttag ctcataaggc cccttccggg
       61 gccttatttc aagatcaaaa gggcggatcc ctctctggga tccgcccttt aatcgccgac
      121 ccgggtcatt tccgcgccgg cccaaatgct ctccagcctg taaaaatctc ttccttcccg
      181 gctgaagatg tttattacga cgttgccggc gtcaagaagg atccatttcg ggctggattc
      241 tccctcaacc ctgtaagttc tcttcatcct gtccatggcc tcggtaagat tatcaagaag
      301 cgtcttcata tgaacatccg agttccccgt cgcgagaatg aaagcatcgg cgaaggatga
      361 ttcccttgaa acgtcgatga tggttatatc ctcggcaagt ttatcctgca gtgcctggat
      421 cagtgggctg acggtatcgt ccggtagcaa aggcaaacct ccctttatta tattatgaga
      481 attctattga tctcgggcca gatcgatcct ttggaaaata ttcatataat catggcccat
      541 tatcattgta acatttctga ccgaagggtc ttttcttact agattctcgg cgatgccgaa
      601 gagcagaccc agcgaccggg cagttttttc agtctcttca ccggggggaa aaaggaggtt
      661 ggtgtattta taatcgtaat gctttgcatt cccaatatgg gccacatcta tcccaaggga
      721 ctggagccgc gtggccgatt ccttgccgac gccggcagta cctgacccgt tcaggatcga
      781 aaggctgacc tttatggatg ccaaggtgtc cttcaggttc aggtccaaat cggtcgcact
      841 caccggttca tcggctctat cctcctccat tatattgtcc tggggttccc cgggtttttc
      901 cgttaggatc tccgagagat cgccgagctc ccccagccag tagctgatcc cggaaatata
      961 ggccgctttt cctggcaggg tgcggaaaac tacccgtgat gggtccaggt ccttaaggta
     1021 tgaggcgagc tggatcgcct gcgaaaggct cagatttgtc ttgacgagac ggatcccctg
     1081 gcggatcagc tcggggatct tggttatcat cgccgggtcc ttgatcttgt tgaagaccgt
     1141 catcatgaag acctgctgcc tttccaccct cccgatatca ccgagggcgt catggcggaa
     1201 cctgacgaaa cccagggcgg ttttcccatc catgtgctgc tcgcccgccg gtatgtcaat
     1261 gtggatcccc ccggccctgt cggtatatct caaccttttg gggacgttga tcgtcacgcc
     1321 gccgacaagg tcaaccagtt ccgggaagtt ctggaggttg acgatgacat ggtacgggat
     1381 gggaacgccc aggtaattca ccagggttgt cttcagaagt tccactcccc cataggcgta
     1441 ggcgtggttc agtttttgcc agcctcgacc gggtatctgt accctcgtgt ccctggggag
     1501 cgatagcaac cggacaacct tgtcgtcgat atcgatcgac acgaaggcga tggaatcggg
     1561 ccggttaaca tcttcgcccg ggactttatc aagacccagc aagagtatat tgatcctccc
     1621 ggtctcctcg tcgtgttcaa ttctttcccg gatggtttcg ggttcggggt tgaaaaagaa
     1681 aaatgctttc aagccgatac ccgccgtcaa cgacaggacc gcggtcagga tcaggaggag
     1741 aacctgtctt tttgacacct atccttcacc atccgttcct gaaatacagg ttattcttgt
     1801 atatatactg ctccaccagc caaggggcaa ggtaccggat actctgaccg ttggagactc
     1861 tcttcctgat ctcggtgctt gagatcgcca ggagaggtat ctcaaggtgg atcaggtttt
     1921 ccctgatctc tacaggcagg ttttcgagcc ctcccgggtt gaagccgggg cggctgacgg
     1981 ctactatcct gcagagccgg gggatctcca ggggctcctt ccaggccggg atctccaaca
     2041 ccgcatcaag gccggttatg aaaaagaact ccacgctgcc ggaagggtac caatgcctca
     2101 tctccctaag cgtgtcgacg gtatggcttg acgtcccgcg gtcgatctct atcctggaga
     2161 tgcggaaatg ggggttgtcg agggtggcga gcatggtcat gacgtaacgg tgttccgccg
     2221 acgatatgga cctccccgcc ttgtggaagg gatcacccgt cggcacaaag accacctctg
     2281 tcaaccccag gctgaaatag gcctcttccc cggcgatcaa atggccgaaa tgtatcgggt
     2341 cgaaggtgcc acccatgacc cctatcttgc gcttgcgcaa accctcggag gaccgtaaag
     2401 gcatgatcgt cacacccccg ttcgacccct cgttccctca tccaccatgg aggatccctc
     2461 gggatagaat tcaaactcca cgtctcctat cctaacagta tcaccttcac gggcaccact
     2521 ttcaaacaga aggtcttcta tccggtaaaa cctcaggatg gcggaaaagc ggctcaaggc
     2581 ctcttcttgt tcgaaatcga acttgccgac gagccgccgt agttcctgat gacgaactat
     2641 gaaggttttc tcgcccttgt gctgctccac gtcgacctcc ggcttttcgg tcttttgcgc
     2701 tgccctgccc tcttcatcgc cgatctttac gaagatggcc tcccgtaggt attcttcccg
     2761 ttccggcatc ctcgaaacga tgtggtccgt cagtgcccgg atcccttccc ccgagagggc
     2821 gctgatagga aagaagggca ctttttccac ttcaagggaa cgctttagtt ccttgatgtc
     2881 cggtaagctc gagggaagat cggtcttgtt cccggcaacg acggagggca gttccagtaa
     2941 cccggggttg aaagctctaa attcggctct cagggtatgc cactggtcgg cgggggtgac
     3001 cagggcttcc ctcgagaggt cgatcacgta gaccaggagg cgggtccttt caacatgctt
     3061 caggaaggcg tgtcctagtc ccctgttcct gtgggcacct tctataagcc ccggaacgtc
     3121 ggccaccacg atcttctttt gatcgaggtc catcaccccc aggttcgggg acagggtcgt
     3181 gaaaggataa tcggcgatct ggggctttga acctgaaatg gcggctagta gcgtcgattt
     3241 tcctgcgttc gggaggccca ccagccccac atcggcaagg atcttcaagt tcaacctgag
     3301 atccctggcc tctcctacct cgccctgttc ggagaagcgg ggggtccttc gccgggatga
     3361 cgcgaacctg gcattccctc tgccgcccct gccgcccttc gctgcgagga aggtatcacc
     3421 ggccacggtg aggtcgcaga agaatcgccc tgtacctttt tcggtaatga tagtcccgca
     3481 agggaccttg acaacgacat cctcaccgtc aggcccggtc tggttccggc ccttaccgtt
     3541 gcctccgttc ccggcgacga actttcttcg gtactcgtag tcggcaaggg tctggatatg
     3601 atcatcggcc acgatccaga catg
//
LOCUS       DAOSRG010000106         3599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_23261_length_3599_cov_7.628950, whole genome shotgun sequence.
ACCESSION   DAOSRG010000106 DAOSRG010000000
VERSION     DAOSRG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_3599_cov_7.628950"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..544)
                     /locus_tag="PLM30_04540"
     CDS             complement(371..544)
                     /locus_tag="PLM30_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypA/HybF"
                     /protein_id="HOR54469.1"
                     /translation="MRKYRCLHCQHEFEVEDLSAGLKCPKCFNRFVQLIEGESVKGKS
                     WGSKSFSVPKMGN"
     gene            complement(651..1331)
                     /locus_tag="PLM30_04545"
     CDS             complement(651..1331)
                     /locus_tag="PLM30_04545"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOR54470.1"
                     /translation="MSVLTALSALWLGVLASVSPCPLAANLAAFSFIQKEGLSPRRVL
                     LSGSLYTLGRTVTFAMAGVVVISGLMAVPEASLFMQRHMNRLIGPILLIVGVFVLDLI
                     EVRGRKPITERVSSGTLFRGMTGAFLMGVLFALAICPVSAALFFGGLIPLAAEKGSRV
                     VLPALFGLGSGLPVTVLSFIFSMGARAVSRSMGTVYNLQKIIQAASGLILILIGLYFS
                     LTSTFGLL"
     gene            complement(1328..1819)
                     /locus_tag="PLM30_04550"
     CDS             complement(1328..1819)
                     /locus_tag="PLM30_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54471.1"
                     /translation="MNYKKIAGYLVLFLAFVGLARAGLDIYSDFARGGNSNAEAVTTG
                     EAWPPEGTGELVIYFFEGNTPCSVCDKIRGMTLDMVKSDPEQLSPFSFRDVNVEEKGN
                     EKYVLELGLFSTSVVLAQESGGKIYRWKNLTGVWDLAADPGAFRDYMLREIDSFLSEG
                     GTR"
     gene            1845..>3599
                     /locus_tag="PLM30_04555"
     CDS             1845..>3599
                     /locus_tag="PLM30_04555"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR54472.1"
                     /translation="MMATCDKYTMKGILVARAEVRARYGRSLFMDEKGNKSKDKNGQE
                     KTTLRITGMTCAACSGAVERSLRKVEGVDFAAVNLATETALVVHGKDVTWEDLQKAVE
                     KVGYGVSDELPEEIDRVRYLNSRRNLVLALAVTVPLMVLMLFHMAGYHLPWFPALELA
                     AAGFVVFYVGRGSIKGAWIAVTHFHTNMDTLISVGAASAWATTLLHVLGVPIASFGSI
                     GAMIVALHISGRFIESSLRDKAAKEIKNLLKLRAVEARAVIDGKEIRVPIEAVVKGMT
                     IKVGPGDRVPVDGEVLSGRSSVDESMITGEPIPVLKEKGASVTGGSSNLTGEMLMTVT
                     RTGEDTFLSKMVSLVQEAQGSKIPIQALADAVTRVFVPVVTALAVTAGVLWYFFFDRF
                     YPLVSSARAYLPWVLVTESRLSFAVFVFVATMVIACPCALGLATPMALVAGTGLATKR
                     GLLIRNAEAIQTTKDIGVVLMDKTGTMTEGNPSVTFHNLSPEDLAAAAAIEKNSGHPL
                     AKAITASAGEDLPAVEDMEEIAGKGVSGVVNGTRYEVGKPASYDAYSGYLEAGKTVVE
                     VDREGVSAGFFAIEDKIRD"
BASE COUNT          774 a         1050 c         1110 g          665 t
ORIGIN      
        1 ggaccggccc aaggcgtgtg gccggacagc gacaagttcc cattcctgaa ggaatggggc
       61 gaggtccact tcctttagga agtgggaaat gtcatcccac ctcaaggttt tgtttggggg
      121 tggatcccat gaagagcgag ggtgacatca ggttgtccat caagcaggcg cggacgcggc
      181 cggttttttc gcgctgcctc tgacttgcct gtgaagaggg aacacgtccc cgaacagggt
      241 gacgggacag ggctaaaagg atgccgctca ggggatgctt tcagaacacg gactttcggg
      301 gtgaaaaaag aagggggccg agttcaccaa gcccccttct tcgttttgag gaaggacgtg
      361 ggtcttgcga tcagttaccc atcttgggga cgctgaaact cttgctcccc caggattttc
      421 ccttgaccga ctcgccctca atgagctgga caaagcggtt gaaacacttg ggacacttaa
      481 gacccgcgga aaggtcctcc acttcgaact catgttggca gtggagacat cggtatttcc
      541 tcacttgcct gcacctccct gcgtcatcgt aagcgtagcc ggacttgctg ctttattata
      601 accgggccgt gggctctaga caagaacaaa aactccccac cggcccacct tcaaaggagc
      661 ccgaaggtgg atgtcaacga gaagtagagg ccgatcagga tcaggatcaa ccctgaagca
      721 gcctggatga tcttctgcag gttgtagacg gtgcccatgc tccgcgatac cgccctggct
      781 cccatcgaaa aaatgaagga aaggaccgtc accgggagac cgctaccgag gccgaaaagg
      841 gcggggagca ccactcggga acctttttcc gcggccaagg gtatgaggcc gccgaagaaa
      901 agggccgccg agaccgggca gatggccagc gcaaagagca cacccatgag gaaggccccg
      961 gtcatacccc gaaagagagt cccggaggat accctttctg tgatgggttt ccttccccgc
     1021 acttcgatca ggtccagcac gaagacgccc acgatcagga gtatgggccc gatgagcctg
     1081 ttcatgtggc gctgcatgaa aagggacgcc tcgggaacgg ccatgagccc cgatatcacg
     1141 accaccccgg ccatggcgaa ggtcaccgtg cgcccaaggg tatagagact tccagaaagg
     1201 agcaccctgc ggggcgaaag cccctccttc tggatgaaag agaaggccgc caggttcgcg
     1261 gcaagggggc aggggctaac cgaggccagc acacccagcc aaagggctga aagggccgtc
     1321 aggacgctca ccgcgttcca ccttccgaca ggaaggagtc tatctcccgg agcatatagt
     1381 ccctgaaagc cccagggtcc gccgccaggt cccagacgcc ggtcaggttt ttccaacggt
     1441 atatcttccc tcccgactcc tgggcgagta cgacggaagt ggagaagagt cccagttcca
     1501 ggacatactt ttcgttcccc ttttcttcca cgttcacgtc ccggaaggaa aagggcgaca
     1561 attgctcggg gtcggacttc accatgtcca acgtcatacc ccttatcttg tcgcagacgg
     1621 agcagggggt attcccctcg aagaagtaaa tgaccagttc accggtgccc tcgggcggcc
     1681 aggcttcacc ggtggtgacg gcctcggcgt tgctgttccc gccccgagcg aagtcactgt
     1741 agatgtcaag acccgcacgg gccagtccta cgaaagccaa aaaaagtacc aggtaaccgg
     1801 cgatcttttt gtaattcatg gtccctccag gttccggccg aataatgatg gccacttgcg
     1861 ataaatatac catgaagggt atactggtcg caagagccga agtgagagcc cgctacggga
     1921 ggtcgctttt tatggatgaa aaggggaaca agagcaagga taaaaacggc caagaaaaga
     1981 ccaccctcag gataacaggg atgacctgcg cggcctgctc cggggccgtt gaaaggtccc
     2041 tgcgaaaggt ggagggggtt gacttcgccg ccgtcaacct ggccacggag accgccctgg
     2101 tggtgcatgg caaggacgta acctgggagg acctgcagaa agcggtcgaa aaggtgggct
     2161 atggcgtctc tgatgagctg cccgaggaga tcgacagggt caggtatctc aattcaagga
     2221 gaaacctggt cctggccctg gcggtgacgg tccccttgat ggttctgatg ctctttcaca
     2281 tggccgggta ccacctcccc tggttccccg cccttgaact ggcggcggcg ggcttcgtcg
     2341 tcttttacgt cggcaggggg agcataaagg gagcctggat cgccgtgacc cacttccaca
     2401 cgaacatgga caccctcatc tcggtggggg cggcctcagc gtgggccacg accctgctgc
     2461 acgtattggg ggtccccata gcctccttcg ggagcatcgg cgctatgatc gtggccctcc
     2521 acatttcggg gaggttcatc gaatcttccc tcagggataa ggcggccaag gagatcaaaa
     2581 acctcctcaa gctgagggcc gtcgaggcca gggccgtcat cgacggcaag gagatccgtg
     2641 tgcccattga agccgtagta aaggggatga ccatcaaagt cgggcccggc gacagggtgc
     2701 ccgttgacgg cgaggttctt tcggggcgtt cttccgtgga tgaatcgatg ataacggggg
     2761 agcccatccc cgtcctcaag gaaaagggcg catccgtaac gggggggtcg tcgaacctga
     2821 ccggcgagat gctcatgacc gtgaccagga ccggcgagga taccttcctc tcgaaaatgg
     2881 tctccctcgt gcaggaggcc cagggttcca agataccgat ccaggcgctg gccgacgccg
     2941 tcacgagggt gttcgtcccc gtcgtcacgg ccctcgctgt aaccgccggg gtcctgtggt
     3001 atttcttttt cgaccggttc taccctctcg tctcttcggc cagggcttac ctcccctggg
     3061 tcctggtgac ggagagcagg ctctccttcg ccgtcttcgt cttcgtggcc accatggtca
     3121 tcgcttgccc ctgcgccctg gggctggcga cacccatggc gctcgtggct gggacgggcc
     3181 tggcgactaa gcgggggctc ctgatcagga acgccgaggc catccagacg accaaggaca
     3241 taggggtcgt cctgatggac aagacgggca ccatgaccga gggcaaccct tcggtgacct
     3301 ttcacaacct ttcccccgag gacctggccg ccgccgccgc catcgagaag aactcgggtc
     3361 acccgctggc caaggcgatc acggcttccg cgggcgaaga ccttcccgcg gtagaagata
     3421 tggaagagat cgccggcaag ggggtcagcg gggtggtcaa cggcaccagg tacgaggtag
     3481 gtaaaccggc ctcctacgat gcctattccg gttatctcga ggccggtaag accgtggtcg
     3541 aggtagaccg ggaaggggtc agtgcgggtt tcttcgccat tgaggacaag ataagggac
//
LOCUS       DAOSRG010000107         3597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_23278_length_3597_cov_10.156126, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000107 DAOSRG010000000
VERSION     DAOSRG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23278_length_3597_cov_10.156126"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLM30_04560"
     CDS             complement(<1..148)
                     /locus_tag="PLM30_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR54473.1"
                     /translation="MKTVAVANQKGGVGKTTCCVNLAAELGRQGKKVLVLDIDPQGNS
                     TSGLG"
     gene            complement(154..855)
                     /gene="rsmG"
                     /locus_tag="PLM30_04565"
     CDS             complement(154..855)
                     /gene="rsmG"
                     /locus_tag="PLM30_04565"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOR54474.1"
                     /translation="MPGSFPEYRLQPEDLLAELQRNRERIEHYVFLLASFNERARLTG
                     PTEPREIMEHIRDCAVSLVFAPDIGAVVDIGTGGGLPGIVWALCRPKLKITLLESVEK
                     KCDILEEMARMLRLPNVEVACARSEALSLERRESYRIALSRGVGHLGVVAEYASPLLG
                     VGGFSYFFKGPKVAEELRDVGDRWATLGFDDPVIYPYDLDGKSLCLVGLEKTAPCPVK
                     YPRRPGKAGKSPWWR"
     gene            1013..>1219
                     /locus_tag="PLM30_04570"
     CDS             1013..>1219
                     /locus_tag="PLM30_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOR54475.1"
                     /translation="MIVERDENLVALRFDDGEDLIPRLLETLGDTTSAVIISAVGMLR
                     DFEIGWLGPEGYEKKRFHEPCEILS"
     assembly_gap    1220..1319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1320..2084
                     /locus_tag="PLM30_04575"
     CDS             <1320..2084
                     /locus_tag="PLM30_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOR54476.1"
                     /translation="AALAACLLLLLTPLQAWPGAALPGRVVRVADGDTVTVATDDGTV
                     AQVRYIGIDCPETHHPRRRVEELGREASRFNESLVLGKRVLLEFDLQRRDRYGRTLAY
                     LWLADEGKGAMINELMVAEGFALPYSFPPNLRHTELFREAFGRARASGKGLWSRARGR
                     VFTPAQVWAELPTVAGHFITVRFKVQEVVSSETRITLRSDKGNASLIVHKSDADQFGP
                     FEESVGKTLTVVGKVTPGFGGPEILLADPSQVLSSW"
     gene            2363..>3597
                     /locus_tag="PLM30_04580"
     CDS             2363..>3597
                     /locus_tag="PLM30_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54477.1"
                     /translation="MTKCWKAFLVLSILLSLAAAGALVLVSKGDIGAGFAVSALGKAA
                     RQQLGAELSLSSLSGNPIKGFTMKTVALRRDGRVLFSAPEVAVVFEPLSLLKGSPRIR
                     SISIGGALFDPGGLPLPSPRERMPGPLALPFDTLLAQKCILRTPRGDLKIEEGKVSLG
                     DGTIGASGSLLYRETPFSVRAELEPVGEELILRRLSVKTAQGSLTGAGRLFPVMDLTG
                     EISDLDVGVLEGFWPELARQDYSGKFSTTFSAKGSWPGVTLEGHLVAPRGEVYGIDVE
                     DLSSSWSFSGDTLEMKEVRGVADGTPLSGTLRFSFSALPPVTVIDITARRAEVKSWAK
                     TFPWLQPLAEGVIDSLDANLAGPSNSLSGSVTLRASELNLAGQPLMGVGGTLSLKDSS
                     TLELDLGAEWLGSAVKGEG"
BASE COUNT          617 a         1134 c         1059 g          687 t
ORIGIN      
        1 cgccaaggcc gctggtgctg ttgccctggg ggtcgatatc gagcacgagc acctttttgc
       61 cctgcctgcc gagctcggcc gcaaggttca cgcaacaggt ggtcttaccc acgcccccct
      121 tctggttcgc gacggcgacg gtcttcaaaa acctcacctc caccagggac ttttaccggc
      181 cttgccgggg cgcctcgggt acttcaccgg gcacggggcg gtcttttcaa gccccaccag
      241 gcaaaggctc ttaccatcca ggtcgtaagg gtagatcacg gggtcgtcaa aaccgagggt
      301 cgcccaccgg tctcccacgt cgcgcagctc ctcggcgacc ttgggcccct tgaaaaagta
      361 ggagaagccg cccaccccca ggaggggaga ggcgtattca gcgacgacgc ctaggtggcc
      421 cacacccctt gagagtgcga tgcgatagct ctcccgccgc tccagggaga gagcctccga
      481 cctcgcgcag gccacctcca cgttgggcag ccggagcatc cgggccatct cttcgaggat
      541 atcgcacttt ttctcgacgc tctcgagcag ggttatcttc agtttcggcc tgcacagggc
      601 ccagacgatc cccggaaggc ccccaccggt cccgatatcc acaacggcgc ctatgtcggg
      661 ggcgaagacc agggaaacgg cgcagtccct gatatgttcc attatctccc ggggctccgt
      721 ggggccggtg agcctcgccc tctcgttgaa agaagccagg agaaagacgt aatgttctat
      781 cctttccctg ttcctctgga gttcggcgag gagatcctcc ggctgcagcc tgtattcggg
      841 aaaggatcca ggcaagctta tccatccttt ccgggaggcc ggcccgcttc gcaccccctc
      901 ccgccctgct atcttacccg aaaaacccgg cgtgtggatg gtgcttttcg atgatagact
      961 ggtataaaag ggtcaaggga cccaaaatat attacgcgag ggagggtaaa ggttgatcgt
     1021 tgaacgggac gagaacctgg tagccctgcg tttcgacgac ggcgaggatc tcatcccccg
     1081 cctcctcgag accctgggcg acactacatc ggcggtgatc atctccgccg tcggcatgct
     1141 ccgcgacttt gaaataggct ggctgggccc cgaaggttac gagaagaaac gattccacga
     1201 accctgcgag atcctctccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1321 cggccctggc cgcctgcctc ctgctccttc tcacgcccct tcaggcctgg cccggggcgg
     1381 ccctcccggg ccgggtcgtg agggtcgccg acggcgatac ggtgaccgtc gccaccgacg
     1441 atgggaccgt cgcgcaggtg cgttatatcg gcatcgactg ccccgaaacc caccaccctc
     1501 gcaggagggt cgaagaactg gggcgggagg cctcccggtt caacgagtcg ctggtgctgg
     1561 ggaagagggt cctccttgag ttcgatctgc agaggaggga ccgctacggg aggaccctgg
     1621 cctacctgtg gctcgccgac gagggaaagg gcgccatgat caacgaactg atggtagccg
     1681 agggcttcgc cctcccctac agttttccac cgaacctgcg ccacacggaa cttttcaggg
     1741 aggccttcgg ccgtgcacgg gcttcgggga agggcctctg gtccagggcc cgggggagag
     1801 tgttcacgcc ggcccaggtc tgggctgaac tgcccacggt ggccgggcac ttcatcactg
     1861 tccgtttcaa ggtccaggaa gtggtctcat cggagacccg catcacccta cgctccgata
     1921 agggcaatgc ctccctcatc gtccacaaga gcgatgccga tcaattcggc cccttcgagg
     1981 agtcagtcgg gaaaaccctc accgtcgtgg gaaaggtcac gccgggtttc ggggggcccg
     2041 agatcctcct ggccgacccg tctcaggtct tgtcttcttg gtgaccttaa acttatccac
     2101 aagtttttcc acatctccac atcacctttc cggtcatttt gacaataatc ggcagtactc
     2161 cacagagaat accccccgag gctgccttgt gcatacaacc ttgacttccc ggagttccag
     2221 gggttcccgg tgtccgtcca taaagagtat tttgaaaacc cttccgccct ttcgggcctc
     2281 taggtattta ccctgattga ttctttcccg ggccgccctt atcatggggt ttcctgtgat
     2341 cgatctccgg gaggttcatc ctttgacaaa atgctggaaa gcatttctgg tcctgtccat
     2401 cctgctctcg ctggctgcag ccggggccct ggtcctggtc tcaaaaggcg acataggggc
     2461 tggtttcgcc gtctccgcac tggggaaagc cgccaggcag cagctggggg cggaactatc
     2521 cctttcatcc ctgtcgggta accccatcaa gggcttcact atgaagaccg tcgcactgag
     2581 gcgcgatggc cgggtgctct tctccgcccc ggaggtggcc gtagtcttcg aacccctttc
     2641 gctgctcaag ggctcaccga ggatcaggtc catctccatt ggcggggcac tgttcgaccc
     2701 gggcggcctt cccctcccgt ccccccggga gaggatgccg gggccgttgg cgctcccctt
     2761 cgatacactc ctggcccaaa agtgcatcct gcggaccccc aggggcgacc tcaagataga
     2821 ggaaggtaag gtttccctcg gagatgggac gatcggcgcg tcggggtccc tcctttacag
     2881 ggagacaccc ttttcggtaa gggcggaact cgaaccggtc ggggaggagc tcatccttcg
     2941 ccggctctcc gtcaagaccg cccaggggag cctgaccggt gcgggacggc tcttcccggt
     3001 gatggacctg acgggagaga tctctgacct cgatgtcggt gtgctggagg ggttctggcc
     3061 cgaactggca aggcaggatt attccgggaa gttctctacc actttctcag ccaaaggttc
     3121 ctggcccggg gtcaccctgg aaggccacct ggtcgccccc cggggcgagg tctatggcat
     3181 cgacgtggag gacctttcct cctcctggtc cttcagcggt gacaccctgg aaatgaagga
     3241 agtcaggggc gtggccgacg ggacgcccct atcggggacg ctgaggtttt ccttcagcgc
     3301 cctgccgccg gtcaccgtca tcgacatcac cgcccgccgg gccgaggtga agtcctgggc
     3361 gaaaaccttt ccctggctcc aacctctggc cgagggggtc atcgactccc tcgacgcgaa
     3421 cctcgccggc ccttcgaact ccctttcagg gagtgtgacc ctcagggcct cggaactcaa
     3481 cctggcgggg cagcccctca tgggggtcgg gggcaccctg tcactaaagg atagttcgac
     3541 cctggagctg gacctggggg cggagtggct cggctccgcc gtgaaggggg aggggag
//
LOCUS       DAOSRG010000108         3560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_23616_length_3560_cov_11.912411, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000108 DAOSRG010000000
VERSION     DAOSRG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23616_length_3560_cov_11.912411"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLM30_04585"
     CDS             <1..411
                     /locus_tag="PLM30_04585"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOR54478.1"
                     /translation="PLEGAGAIDAREIPDLIPVLAVTAAFARGKTLFEGAARLRLKES
                     DRLESTASMIRALGGDAREGPDRLEVTGGAPLEGGVVDCRGDHRIAMAGAVAALGCRN
                     ETVLLGAEAVNKSYPSFFQDYRSLGGDCDVIDDR"
     gene            392..>1452
                     /gene="aroC"
                     /locus_tag="PLM30_04590"
     CDS             392..>1452
                     /gene="aroC"
                     /locus_tag="PLM30_04590"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOR54479.1"
                     /translation="MSSMIGEKLRVSLFGQSHGPAVGVVMDGLPAGEEVDLDELRSFL
                     RRRAPGGGPLSTPRVEKDEPRFLSGLAGDRTCGAPLCAVIENTDARPEDYREVATAPR
                     PSHCDYPARLRYGGYEDARGGGHFSGRLTAPLCVAGGVAIQILKRRGIHLGAHAEQVG
                     SARDRRFHPTGVTAGDLALPATRRIPVLDETRMEAMEREILAAAAEGDSVGGVVECAA
                     LGVPAGIGDPIFGGMENRLAAALFGIPGLRGVEFGTGFAAAEMRGSRHNDPYRLKDGR
                     VVTGTNHHGGILGGITTGMPLVFRVAVKPTSSIAMEQKTVDLATMTERTIRVPGRHDP
                     CIVLRAVPCVEAVAGIVLL"
     assembly_gap    1453..1552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1553..2275
                     /locus_tag="PLM30_04595"
     CDS             <1553..2275
                     /locus_tag="PLM30_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOR54480.1"
                     /translation="YLRLLYSVMFDLSRSYQAALGEGETELGRRIKRAVAETAPRFPR
                     RATVACPGEQGSCSQAACDRFFSMPEVFYFRSFEGVFQAVAQGLCDFGVLPVENGTGG
                     SMDAVYELLHRFGFHIVRGTRLRAPAGECGPAEGGGTGDTRFICVARELQIYPGSDRV
                     SLMLTLPHSSGSLYRVIARFAALDLNLVKLESRPIAGNDLEYLFFIDLEASVWSDEVV
                     RALGECASSGRLFDFLGSYREI"
     gene            2278..3507
                     /locus_tag="PLM30_04600"
     CDS             2278..3507
                     /locus_tag="PLM30_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOR54481.1"
                     /translation="MRYGLLGEKLGHSHSPVIYRELGIDDYQLFELPPEGVEPFLRGG
                     GLAGLNVTIPYKKRVLPFCDTLTETARRLGNVNLLLFDDEGKIHGDNTDYGGFLYLLR
                     RAGIDPAGKKVLVLGSGGAARTVTLACLDSGAGEVVQVSRSGPDNYGNIHRHADARVL
                     VNATPVGMHPLADEAPLGLEGFTALEAVVDLIYNPLRTGLLQEARRRGIPCSCGLPML
                     VEQGRLAAETFLGGDIPPEKSEEIHAALLRAVENIVLVGMPGSGKTSVAERVALETGR
                     KRLDTDEMVRELTGRDPADLIERDGEGAFRRIEEEVVRKASGESGSVIATGGGAVLSG
                     ANRAALRRNGRVYWLDRPLADLPLEGRPLSKDLDRLYEERRPLYGEAADARIDASRGV
                     LPAARAVLEEFHAHTGA"
BASE COUNT          536 a         1195 c         1217 g          512 t
ORIGIN      
        1 cccctcgagg gagccggggc catcgacgcc agggagatcc ccgacctcat ccccgtcctg
       61 gccgtgacgg ccgccttcgc ccggggaaag accctcttcg agggggcggc gcgcctcagg
      121 ctcaaggaaa gcgaccggct cgagagcacc gcttcgatga taagggccct gggcggcgac
      181 gcccgggagg ggcccgaccg cctcgaggtg acggggggcg cccccctgga gggaggcgtc
      241 gtggattgcc gcggcgacca ccgcatcgcc atggcggggg ccgtcgcggc cctgggctgc
      301 cgcaacgaga cggtcctcct gggggcggaa gcggtgaaca agtcctaccc ttcctttttc
      361 caggattacc gaagtttggg gggagattgc gatgtcatcg atgataggtg agaagctccg
      421 ggtttccctg ttcggccagt cccacgggcc ggccgtcggg gtcgtgatgg acggcctgcc
      481 tgcgggcgag gaggtcgacc tagatgaact gcggtccttc ttaaggcggc gcgcccccgg
      541 agggggaccc ctttcgacgc cccgcgtcga gaaggacgag ccccgcttcc tctccgggct
      601 cgcgggagac cgcacctgcg gcgcccccct ttgcgcggtc atcgagaaca ccgacgccag
      661 gccggaggac taccgcgagg tcgcaacggc accccgcccc tcccactgcg actacccggc
      721 ccgcctccgg tacggggggt acgaggacgc ccggggaggc gggcattttt ccggccggtt
      781 gacggcaccc ctctgtgtag ccgggggggt ggcgatacag atcctgaagc gccgcggcat
      841 ccacctgggg gcccatgccg aacaggtggg ctccgccagg gacaggcggt tccacccgac
      901 gggcgtgacc gcgggcgacc tcgccctgcc cgcgacccgc cggatccccg tcctcgacga
      961 gaccaggatg gaggccatgg aaagggagat cctcgcggcg gccgctgagg gcgacagcgt
     1021 gggcggcgtc gtggagtgcg ccgccctcgg cgtgccggcg gggataggcg accccatatt
     1081 cggcggcatg gagaaccggc tcgccgcggc gctcttcggc atccccggtc tgcggggggt
     1141 cgagttcggc acggggttcg ccgcggcgga gatgaggggc tcccggcaca acgaccccta
     1201 ccgcctgaag gacgggcggg tggtcaccgg gacaaaccac cacgggggga tcctgggcgg
     1261 gatcaccacc ggcatgcccc tggtcttccg cgtggccgtg aaaccgactt catccatagc
     1321 catggagcag aagaccgtcg acctcgccac catgacggaa aggacgatcc gcgtgcccgg
     1381 aaggcacgat ccctgcatcg ttttgcgggc cgtcccctgc gtggaggccg tcgcgggcat
     1441 cgttctgctg gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntacctccg
     1561 gttgctctac tcggtcatgt tcgacctgag ccgctcctac caggcagccc tcggggaagg
     1621 ggagacagaa ctggggcggc gcatcaaaag ggccgtcgcc gaaacggcgc cccgctttcc
     1681 ccgccgggcc accgtcgcct gccccgggga gcaggggtcc tgttcccagg ccgcctgcga
     1741 ccgcttcttc tccatgccgg aggtcttcta cttccgcagc ttcgaggggg tgttccaggc
     1801 ggtggcccag gggctgtgcg acttcggcgt cctgcccgtc gagaacggca ccggcggttc
     1861 gatggacgcc gtctacgaac ttctgcaccg cttcgggttc catatcgtgc gcggcacccg
     1921 gctgcgcgcc cccgccgggg agtgcggccc ggcggagggc ggtgggaccg gcgacacccg
     1981 cttcatctgc gtcgccaggg aactgcagat ctaccccggg tcggaccggg tgagcctgat
     2041 gctcaccctg ccccactcat cggggtccct ctaccgcgtg atcgcccggt tcgccgcgct
     2101 ggacctcaac ctggtcaaac tcgaaagccg ccccatcgcc ggcaacgacc tggagtacct
     2161 tttcttcatc gacctggagg cctcggtttg gtccgacgag gtggtgcgtg ccctgggcga
     2221 gtgcgcctcc tcgggacgcc tcttcgactt cctcggcagt tacagggaga tctgacgatg
     2281 cgctacgggc tgctggggga gaaactgggg cacagccact cccccgtgat ctacagggaa
     2341 ctgggcatcg acgactacca gctgttcgag ttacctcccg aaggggtgga gcccttcctg
     2401 cggggcggcg gcctggcagg gctcaacgtg accatcccct acaagaaaag ggtgctcccc
     2461 ttttgcgata ccctgactga gaccgctcgg cgcctgggga acgtgaacct cctcctcttc
     2521 gacgacgagg ggaaaataca cggcgacaac accgactacg gcggcttcct ctacctcctc
     2581 cggcgtgccg gcatcgaccc ggcgggcaaa aaggtcctgg tgctgggctc cggcggcgcc
     2641 gcccggacgg tcaccctcgc ctgcctcgac tcgggggcgg gggaggtggt ccaggtctcc
     2701 cgctccgggc cggacaatta cggcaacatc catcgccacg ccgacgcccg ggtcctggtg
     2761 aacgccaccc ccgtcggcat gcaccccctg gccgacgaag caccgctggg cctggagggc
     2821 ttcacggccc tggaggccgt ggtggacctc atctacaacc ccctgaggac cgggctcctg
     2881 caggaagcgc ggcggcgggg gatcccctgc tcctgcggcc tgcccatgct cgtcgagcag
     2941 gggcggctcg cggcggaaac cttcctcggc ggggatatcc ccccggaaaa gagcgaagag
     3001 atccatgccg ccctgctgcg ggcggtggaa aacatcgtac tggttgggat gccgggcagc
     3061 ggcaaaacct cggtggccga gcgggtcgcc cttgagacgg gccggaaaag gctggacacc
     3121 gacgagatgg tgagggagct gacggggcgg gacccggcgg acctcatcga acgcgacggg
     3181 gagggggcct tccgccggat cgaggaggag gtcgttcgca aagcctccgg tgagagcggg
     3241 tccgtcatcg ccaccggcgg gggcgccgtc ctgagcggag cgaaccgggc ggccctccgc
     3301 cggaacggcc gggtctactg gctcgaccga cccctggcgg acctccccct cgagggacgc
     3361 cccctgtcaa aagacctgga caggctttac gaggagcgcc gccccctcta cggggaggcc
     3421 gccgacgcga ggatagacgc ctcccggggg gtgctgccgg ccgcccgggc ggtcctggag
     3481 gaattccatg cgcatactgg tgcttaacgg gccgaacctg aacctcctcg ggaccaggga
     3541 gcccgatatc tacggcgaaa
//
LOCUS       DAOSRG010000109         3508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24062_length_3508_cov_9.337677, whole genome shotgun sequence.
ACCESSION   DAOSRG010000109 DAOSRG010000000
VERSION     DAOSRG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24062_length_3508_cov_9.337677"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLM30_04605"
     CDS             complement(<1..510)
                     /locus_tag="PLM30_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOR54482.1"
                     /translation="MEESGKRSINGGLVVLGVALAVGMVLSSWLVSDTVKSVKLANQT
                     IAVKGTAQVDVRSDIALWTGRFTARDADLVKAYSKLESDLEKVLGFLGRSGIPREEIE
                     VSAVTTMIQYRKTSQGYDTNEIEQYVLDQTVTVRSKEVDLVASLSRESTTLIREGVEF
                     FSFSPEYYYS"
     gene            complement(516..1043)
                     /locus_tag="PLM30_04610"
     CDS             complement(516..1043)
                     /locus_tag="PLM30_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOR54483.1"
                     /translation="MAARKRARCSRQGLSKVGSLLMASLSREQASMLLFADLCSRWCE
                     VVGPKLAGRSSPGHLIDGTLYVTASDPLLCQEITMRGGDIAFRVKEKWGLEVSGVLPR
                     VGRPRGRRSSEKRRGESKPLRPVAGEVAACLEEVRPAVGRDDVAADLARLMALYRKKF
                     GSEGGLPRHKTGKEG"
     gene            complement(1015..>1329)
                     /locus_tag="PLM30_04615"
     CDS             complement(1015..>1329)
                     /locus_tag="PLM30_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOR54484.1"
                     /translation="PLDREITERAQVEIKYEGYVARQLSQVERMKRMELVRVPEGLDY
                     SSVTGLLDESRQKLEAIRPRNLGQAGRISGVTPADIMLLEVFIERLRRGRGNGGQEKG
                     EV"
     assembly_gap    1330..1429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1430..2778)
                     /gene="mnmG"
                     /locus_tag="PLM30_04620"
     CDS             complement(<1430..2778)
                     /gene="mnmG"
                     /locus_tag="PLM30_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOR54485.1"
                     /translation="MRHDRPFDVIVVGGGHAGCEAALASARMGARTLLLNLLLDNAAM
                     MPCNPSIGGPAKGHLTREVDALGGEQGAAADASTLHIRMLNTSKGAAVRALRAQCDLG
                     DYHRWYRRACDGQAGLEVMQDEVTGLWVDGGKVRGLRTRMGTLYEARAVVIATGTFLG
                     GRVHIGQVSFPSGPLGQVSAEALGADLREAGLRVGRLRTGTTPRIHAGSVDWGSLTRQ
                     ESDAEPHCFSHWGTSKVYSGHACYSTRTGEETHRIIRDCLERSPGTLEMGEVKGPRYC
                     TSIEERVARFPEKESHPVFLEPVGRESVEIYMQNFTTGLPLDAQLKMVRSLPGCGKAR
                     IMRPGYGIEYDFILPTQLEPWLEVKAIGGLFCAGQVNGTSGYEEAAAQGILAGINAAL
                     STRGEEPLVIERSRGYLGVLVDDLVTRGTDEPYRMLTSRCEYRLLLRHDNADRRLAPV
                     "
     gene            complement(2768..>3508)
                     /locus_tag="PLM30_04625"
     CDS             complement(2768..>3508)
                     /locus_tag="PLM30_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOR54486.1"
                     /translation="LRPLVPSLIFLPNDLSLVEGSPATRRRLVDRLCALLFPVYALRL
                     HEYGKALRQRNAALRQGRNLAATARVMAPLAAWVWACRLTATDLLRNGLESGGGLLPR
                     GVTVELKRGSKGLEDLHLDFWESLEACQGQERGMGACPVGPHRDDLLIRYGEKEVARC
                     FSRGYRRRVAIALLISSGWAVERKMRRKPILLLDEVMAELDLEGRERLIEVLLSTGWQ
                     VFAATAEACTPEWPGKVWSVEGGRVDAA"
BASE COUNT          524 a         1193 c         1073 g          618 t
ORIGIN      
        1 cgagtagtag tactccggcg agaaggagaa gaactccacc ccttccctta tcagggtggt
       61 ggactccctc gagagggacg ccaccaggtc gacttccttt gacctcaccg tgacggtctg
      121 gtccagtacg tactgctcga tctcgttggt gtcgtatccc tgggaggtct tgcggtactg
      181 gatcatggtg gtgacggccg agacttcgat ctcctccctg gggatgccac tccggcccag
      241 gaagcccagc accttctcca ggtccgactc gagcttggag taggccttga ccaggtccgc
      301 gtccctggcg gtgaagcgcc ccgtccaaag ggctatgtcc gaccggacgt ccacctgggc
      361 ggtgcccttg acggcgatcg tctggttggc cagttttacg ctcttcaccg tgtccgacac
      421 gagccacgac gaaaggacca ttcccaccgc aagggcgacg cccaaaacga cgagcccccc
      481 gttgatggac ctcttcccgc tctcctccat ggcgatcagc cttcctttcc cgttttgtgg
      541 cggggcagcc cgccttcgga accgaacttc ttcctgtaaa gcgccatgag cctcgccagg
      601 tccgccgcga cgtcgtccct tcccaccgcg ggacggacct cctccaggca ggcggcgacc
      661 tcaccggcga cgggcctcag aggtttggat tctccccttc ttttctcaga ggatctcctg
      721 ccgcggggtc tccctaccct ggggaggacc cccgagacct cgaggcccca tttttccttc
      781 accctgaagg cgatgtcccc gcctctcatg gttatctcct ggcacaggag ggggtccgag
      841 gctgtgacgt aaagggtgcc gtcgatcagg tgccccgggg aactcctccc ggcgagcttc
      901 ggccccacca cctcgcacca ccgggagcag aggtcggcga agaggagcat cgaagcctgc
      961 tctcgggaga gggaggccat gaggagcgac ccgaccttcg aaagaccctg ccggctacac
     1021 ctcgcccttt tcctggccgc cattgcccct ccccctccgg agacgttcga tgaaaacctc
     1081 cagcaacatt atatccgctg gcgtcacccc cgatatcctc cccgcctgcc caaggttcct
     1141 ggggcggatc gcctccagtt tctgcctgct ctcgtcgagg aggcccgtca cggaagagta
     1201 atccaacccc tcgggtacgc gcaccagttc catcctcttc atcctctcca cctgggagag
     1261 ttgccgggcc acgtacccct cgtacttgat ctccacctgg gcccgttccg tgatctcccg
     1321 gtcaaggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgggggccaa
     1441 ccgcctgtcg gcgttgtcgt gcctcaggag gagcctgtac tcgcagcggc tggtgagcat
     1501 cctgtagggt tcgtcggtcc cccgggtgac gaggtcgtcc acgagcactc ccaggtaccc
     1561 ccgggacctc tctatcacca gcggctcctc cccccgggtc gaaagggcgg cgttgatgcc
     1621 ggccaggatc ccctgggcgg cggcttcctc gtagcccgag gtgccgttca cctgtcccgc
     1681 gcagaagaga cccccgatgg ccttcacttc cagccagggc tccagctggg tggggaggat
     1741 gaagtcgtac tcgataccgt agccgggcct cattatcctg gccttcccgc atccggggag
     1801 ggaccggacc atcttcaact gggcgtcgag ggggaggccg gtggtgaagt tctgcatgta
     1861 gatctcgacg ctctccctgc cgacgggttc gaggaagacc gggtgactct ccttctcggg
     1921 gaagcgggcg accctctcct cgatggaggt gcagtacctg ggccccttga cctcgcccat
     1981 ctcgagggtg ccgggcgacc tttccaggca gtccctgatg atccggtggg tctcctcgcc
     2041 ggtcctggtg gagtagcagg cgtggccgct gtagaccttc gaggtccccc agtggctgaa
     2101 gcagtggggt tcggcgtcgc tctcctgcct ggtgagggag ccccagtcga cgctgccggc
     2161 gtggatcctg ggggtggtcc ctgtcctcag cctcccgacc cggaggcccg cctcgcggag
     2221 gtccgcgccc agggcctcgg cggagacctg tcccagcggc cccgacggga aggagacctg
     2281 cccgatatgg accctcccgc ccaggaaggt ccccgtggcg atgacgaccg cccgggcctc
     2341 gtagagggtt cccatcctcg tcctcaggcc cctgaccttc ccgccgtcga cccaaaggcc
     2401 ggttacctcg tcctgcatga cctccagccc ggcctggccg tcgcaggctc ttctatacca
     2461 gcggtggtag tcgcccaggt cgcattgggc cctcagggcc ctgacggcgg cacccttgga
     2521 ggtgttgagc atcctgatgt gaagggtgga ggcatcggcg gcggcgccct gttcaccgcc
     2581 gagggcgtcc acctcccggg tgaggtgccc cttggcaggc cccccgatgg aggggttgca
     2641 gggcatcatg gccgcgttgt ccagcaggag gttgagcagg agggtcctcg cccccatcct
     2701 ggccgaagcc agggcggcct cgcacccggc gtggccgccg cccaccacta tgacatcgaa
     2761 gggacggtca tgccgcatcg acacgccccc cttccacgga ccagaccttc ccgggccatt
     2821 cgggggtgca ggcctccgcc gtggcggcga agacctgcca ccccgtgctg aggaggacct
     2881 cgatgagccg ttcccttccc tcgaggtcca gttcggccat tacttcgtcg aggaggagga
     2941 tgggctttct ccgcatcttc ctctccacgg cccagcccga ggagatcaag agggctatgg
     3001 cgaccctcct cctgtagccg cggctgaaac accgggccac ctccttttcg ccgtagcgga
     3061 tgaggagatc gtccctgtgg ggcccgacgg ggcaggcgcc catgcccctc tcctgaccct
     3121 ggcaggcctc gagggactcc cagaagtcaa ggtgaaggtc ctcgagcccc ttgcttcccc
     3181 tcttgagttc cactgtgacg ccccggggaa gcaacccgcc gccgctctcg aggccgtttc
     3241 ggagcaggtc cgtggcggtg agccggcagg cccataccca ggcagcgagg ggggccatga
     3301 ccctggcggt ggcggccagg ttcctgccct gccggagggc ggcgttcctc tgcctcaggg
     3361 ccttgccgta ctcgtgaagc ctcagggcgt aaacagggaa aaggagggcg caaagacggt
     3421 ccacgagccg ccttctcgtc gctggagaac cctccaccag ggagaggtcg ttgggaagga
     3481 agatcagcga ggggaccagg ggcctgag
//
LOCUS       DAOSRG010000110         3462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24462_length_3462_cov_7.620781, whole genome shotgun sequence.
ACCESSION   DAOSRG010000110 DAOSRG010000000
VERSION     DAOSRG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24462_length_3462_cov_7.620781"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..919)
                     /locus_tag="PLM30_04630"
     CDS             complement(155..919)
                     /locus_tag="PLM30_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54487.1"
                     /translation="MKKMILILFLAVLVILPAPPGRAEAAMSYEELLEVYSRKFENRP
                     KAEALRSTLLEMAWDSGGPTLLGSIKDPGLPPEQRAANGLKLIEVLFPNGDPARWEGV
                     SGFWSGPMIPKPLAAFDAAFFTVMALLEMDRPEAPWVAQDLLQALRSSSAAALLALRT
                     APAEYPWIVGALEKGTGLPPLGGWPRGKVRGKLPFAHPVRSVITEIQAQSRDMLFLNS
                     AGQPAPGGPYAWDRDRGRVYRVIEPSDDQYWWILPD"
     gene            1138..>2222
                     /locus_tag="PLM30_04635"
     CDS             1138..>2222
                     /locus_tag="PLM30_04635"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOR54488.1"
                     /translation="MNGLAFDGVIERRGTDSLKWDGMEGRFGPVPGDALPLWVADMDF
                     LSPPEVIEAIRRRALHGVFGYVQASPGSYQAVSDWLRINHGWHTDPSWVVSCSGVMPA
                     IGAAIRAFSDPGDGVIIQPPVYPPFFRIVKDNGRQVVENPLINDGDRYRMDMEGLRVL
                     LEEGKNRIFLLCSPHNPVGRVWTREELDGVCRLCRETGTLLVSDEIHADLVFKPFAHI
                     PAASISPEKADRIITCVSPSKTFNIPGLQTAYTVIVDPGLREGFQASARAAGIGAQNV
                     FGLAASEAAYRNGAPWLRSVLHYLKENIDRSLAFFQEEMPWVKTRSPEGTYLLWLDMS
                     ASGLAPEAVTERLIQKGKVVLDPGYWFG"
     assembly_gap    2223..2322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2323..2594)
                     /locus_tag="PLM30_04640"
     CDS             complement(<2323..2594)
                     /locus_tag="PLM30_04640"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOR54489.1"
                     /translation="MNNLENAASHCSAMIERSNRIVLLSGAGMSTSAGLPDFRGPDGI
                     YRRKLGVDPEKIFDIDWFRRDPSFFYRFHREFLGSLEKIEPTFAHR"
     gene            2727..>3462
                     /locus_tag="PLM30_04645"
     CDS             2727..>3462
                     /locus_tag="PLM30_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOR54490.1"
                     /translation="MARKSIVYGVNDKPPAPIMVLAGLQHVLTLFGATTLVPLIFGPA
                     MGMTTSQIAFFISCVYFAMGIATLIQTHPKLGSGLPIVQGSSFSFIPPIMTIIGAYSG
                     FGPNVVMQYIGGALISGGLILAGLGYSRLVGYIRKIITPVVIGPTIMAIGFSLASTAV
                     QSNAANYWPVSLIVVACVFIFSLLSRNRFMNIFAVLASIVIAYLVCLFGSLAGLFPAG
                     HAAHVSLAEVRLAPWFRLNLFMPWGPP"
BASE COUNT          633 a         1058 c          940 g          731 t
ORIGIN      
        1 tttcaatcca aaccagcgga ttatagatca gtattgttgt atatgcaacc tttttaccgc
       61 ttttcatttg atactttccc tggccaaatt tatattccac accacgacct ggccaggcga
      121 ggctcgacgg atcatccatg gaccggcacc atcgtcaatc aggcaggatc caccagtatt
      181 ggtcgtcgct gggttctatc acccggtaga cccttccccg gtcgcggtcc caggcgtagg
      241 gaccccccgg ggctggttgt ccggccgagt tgaggaacag catatcccgc gactgcgcct
      301 gtatttcggt gatcacggac cttacggggt gcgcgaaggg gagcttgccc cggacctttc
      361 ccctcggcca gccccccagg gggggcaaac ccgtaccctt ttcgagggct cccactatcc
      421 acgggtattc cgcaggggcc gtcctcaaag ccaggagtgc cgccgccgac gaactcctga
      481 gcgcctggag aaggtcctgg gccacccagg gagcctccgg gcggtccatt tcgagcaggg
      541 ccataacggt gaagaaggcc gcgtcaaaag ccgcaagggg tttcggtatc atcggccccg
      601 accaaaagcc cgagacccct tcccatcggg cagggtcgcc attggggaag agcacctcga
      661 tcagtttgag gccgttggcg gctctctgtt cagggggaag acccgggtcc tttatagaac
      721 ccaacagggt cgggcccccg gagtcccacg ccatctccag gagcgttgat ctcagggctt
      781 ccgctttcgg tctgttttca aacttcctgg agtacacttc cagcaactct tcgtagctca
      841 tggcggcctc agcccgtcca gggggggccg gcagtatgac cagtacggcc aggaacagga
      901 taaggatcat ctttttcata ggatccacct ccaagtcttt caatgatccc catacgactc
      961 tattttaaac cgctttcgcc tcaggggaat gtagcggctc ctgtagtatt atgggtccgc
     1021 ccctggaggt cctgatctga agaccgaaag gtacgacgga agccgtccgg ggggattttt
     1081 ccctaacggg agaaaagcat aaggggggtt cgaccagtgt caggtggagc tatcggtttg
     1141 aatggtcttg cgttcgacgg tgtcattgaa agaaggggga ccgacagcct caaatgggac
     1201 gggatggagg ggcgtttcgg ccctgtccca ggggatgccc tgcccctgtg ggtcgcagat
     1261 atggattttc tctccccccc ggaggtcatc gaagccatca ggcggagggc tctccacggt
     1321 gtcttcggct acgtgcaggc ctcccctgga tcctaccagg ccgtgtccga ctggctccga
     1381 ataaaccatg ggtggcacac ggacccctcc tgggttgtgt cctgctcggg ggtcatgccg
     1441 gccatcggcg ccgccatcag ggccttttcg gatcctggtg acggcgtgat catccagccc
     1501 ccggtgtacc cgcctttttt caggatcgtc aaggacaacg gcaggcaggt ggtggaaaac
     1561 ccccttataa acgacgggga ccgttaccgg atggacatgg aaggcctgag ggtcctgctt
     1621 gaagagggca aaaaccgtat ctttctcctt tgcagccccc acaatcctgt ggggagggtg
     1681 tggacaaggg aggaactcga cggggtctgc cgcctctgcc gggaaacggg gacccttctc
     1741 gtatccgacg agatccacgc cgacctggtc ttcaaaccct ttgcccatat cccggctgcc
     1801 tccatctcgc ctgaaaaggc cgaccgtatc attacctgtg taagtcccag caagaccttc
     1861 aacatccccg gtctccagac ggcctacacc gtcatcgtcg atcccgggct gcgcgagggt
     1921 ttccaggcgt cagcaagggc cgcaggaatc ggcgcccaaa acgtcttcgg tcttgccgct
     1981 tccgaagcgg cctaccgtaa cggcgcgccc tggctcagat cggtgcttca ttacctgaag
     2041 gagaatatcg accgctccct ggctttcttc caggaggaga tgccctgggt taagacccgc
     2101 tcccccgaag ggacatacct cctctggctg gacatgtccg cctcggggct ggcccccgag
     2161 gccgtcacgg agaggcttat ccagaaaggg aaggtcgtgc ttgaccccgg ttactggttc
     2221 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaacggtg agcgaaggtc
     2341 ggctcgatct tctccagtga ccccagaaat tcccggtgga accggtagaa gaaggaggga
     2401 tccctcctga accaatcgat gtcgaagatc ttctccgggt cgacccccag ttttctccgg
     2461 tatatcccgt cggggcccct gaaatccggc aaccccgcgc tcgtggacat ccccgccccg
     2521 ctgagaagga cgatccggtt tgacctctcg atcatggccg aacaatgaga agcggcattc
     2581 tcaaggttat tcatcgggcc agcctccttt tttctttcgt cgtccctaac aggataaccc
     2641 tttccgattt ttttgtgcaa ccggttacat aaaatgctac aatgcacgcc ggagtcttgg
     2701 acatcatcag ggaggtgttc catattatgg ccaggaagag cattgtgtac ggagtgaatg
     2761 acaagccccc ggcaccgatc atggtcctcg ccggtctgca gcacgttctt actctcttcg
     2821 gggcgaccac ccttgtaccc ctgatctttg gccccgccat gggtatgacg acgtcccaga
     2881 tcgctttttt catttcctgc gtctacttcg ccatgggtat agcgaccctg atccagaccc
     2941 accccaagtt aggttcgggt ctgcccatcg tacagggttc cagcttcagt ttcatacccc
     3001 cgataatgac cattatcggg gcctacagcg ggttcggccc caacgtggtc atgcagtaca
     3061 taggcggggc gctcatatca ggcgggctca tcctggcggg gctcggttac agccgcctag
     3121 tgggctatat ccgcaagatc atcacccctg tcgtcatcgg ccccacgatc atggccatcg
     3181 gattttccct ggcgtccacg gcggtccagt ccaacgccgc caactactgg cccgtttccc
     3241 tgatcgtcgt cgcctgcgtc ttcatcttca gcctcctgag ccggaacagg ttcatgaaca
     3301 tcttcgcagt gcttgcctcc attgtcatag cctacctcgt ctgcctcttc ggcagcctcg
     3361 cgggtctttt ccctgccggg cacgccgctc acgtcagcct ggccgaagtg aggctggccc
     3421 cctggttcag gctcaacctg ttcatgccct ggggccctcc cc
//
LOCUS       DAOSRG010000111         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24731_length_3433_cov_7.939609, whole genome shotgun sequence.
ACCESSION   DAOSRG010000111 DAOSRG010000000
VERSION     DAOSRG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24731_length_3433_cov_7.939609"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..493
                     /gene="rbfA"
                     /locus_tag="PLM30_04650"
     CDS             119..493
                     /gene="rbfA"
                     /locus_tag="PLM30_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR54491.1"
                     /translation="MPTFRIERINRELLRRISDLIRTRIKDDSLREAVLSEVVCSKDL
                     SHAKVYFRTLTPGSREKVEASLNRTAGVLRSMLGKGMRLRQVPELHFVYDTTEDRAQA
                     IENILDSLGLPGAVREGEDAGS"
     gene            490..1479
                     /locus_tag="PLM30_04655"
     CDS             490..1479
                     /locus_tag="PLM30_04655"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR54492.1"
                     /translation="MKDLQGMIDLLESVPEWVIVAHEKPDGDTLGCGSALFLQCESLG
                     KKCSWFGPDPFPKAYRFLPFHEKYVAAGSLENSLRSEDLAVIVTDTSNPERTLGGLPS
                     PEDRAVVLNIDHHGDNTRYGTFNWVDDRASSVGLMIYELFLTARWDLTRRTAGSLFTA
                     VATDTGFFRFPSTDERTLRIASDLVAKGAEPHRVYQEVYENRSLEGIHLWGRGLSRAS
                     LHLDGRVCVTYLSREDLSSLGASRDDTENLVNALLSVKRVTVAALFQEDEGACRVSIR
                     TKEPVNARKIAALWGGGGHERASGCKMEGNPERSLEAFVTRLGDLDEIGISDH"
     gene            1454..2377
                     /gene="truB"
                     /locus_tag="PLM30_04660"
     CDS             1454..2377
                     /gene="truB"
                     /locus_tag="PLM30_04660"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR54493.1"
                     /translation="MRSGFLIIDKAPGTRSSQCVALVKRALGGKERVGHGGTLDQPAS
                     GVLALLIGRATRASRFVMMLPKIYLAVARLGLRTDTDDLSGAVISEEDTGLVSEESID
                     RALFSLYGHRDQTPPAFSAVKVKGRRAHAMAREGLDVPLSARPALVTSIGRTGPIMAG
                     RDVPLRIVCRQGTYVRSIVRDLGETLGCGASVLSLRRESLGPFAAPDALPSGDLATAD
                     ASTLEGRILPFSSLSGSFTYYQASLADEGTIASGSPLSLGKLARRGWGGHGTGGYILI
                     FGKAMVSFARVVHEDGVYLVRPVTNIILEGP"
     gene            2381..3301
                     /locus_tag="PLM30_04665"
     CDS             2381..3301
                     /locus_tag="PLM30_04665"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOR54494.1"
                     /translation="MIAVVGSFDGFHLGHRKLFEAAENLSRVMSDSWCVVTFFPHPQL
                     VLGNKTFLPLFTEPEKDILGKCLGIPEIVRLDFSKDLAGMEPDDFLGFLEDRLFIRGL
                     VVGEDFRFGRGRRGGAELLKELASRRGWEVEIVPHLAAPSGRKIGSSFIRESVIRGDL
                     PQASADLGHPFMIRGLIVRGDGRGRTIGFPTINLSLPGMKVLPARGVYAGGVAFGKDA
                     LPAAVNIGLNPTFPGGEGIRCEAYIPGFRGELYGREVSLFLFRRLRREKAFPAVSLLV
                     EQMKIDVKDTLAQWQGTCEGTKRFMACCGL"
BASE COUNT          703 a          947 c         1086 g          697 t
ORIGIN      
        1 ctctcgtcct cccatcacga agcggaagaa aggctcgact cgataatacg gctggtccgt
       61 tcgatggaag aaaagggaga cttctttctt gtccgcgaga agaaagaggt aaaaggatat
      121 gcctactttc aggattgaaa ggataaacag ggagctgctc cgccggatct cagacctgat
      181 aaggacgagg ataaaggatg attccctgag ggaggctgtc ctttcggagg tggtctgcag
      241 caaggacctc tcccacgcca aggtttactt caggaccctt accccgggca gccgggaaaa
      301 agttgaggct tcccttaaca ggacggcggg ggtgctgaga tccatgctag gtaagggtat
      361 gcgtctgaga caggtgcccg aacttcactt tgtttacgac accaccgagg accgtgcaca
      421 agccatagag aacatcctcg acagcctggg cctccccggg gctgtgagag aaggggagga
      481 tgccggctct tgaaggacct ccagggaatg atcgatctcc ttgaaagcgt tccggagtgg
      541 gtcatcgtgg cccacgagaa acccgacggc gatacgctgg gttgcgggag cgcccttttt
      601 cttcaatgtg aatctctggg gaagaagtgt tcctggttcg gtcccgaccc tttcccgaag
      661 gcttaccggt tcctgccctt ccatgaaaag tacgtcgcag cgggatcctt agaaaacagt
      721 ttgcgttcag aggacctggc cgtgatcgtg acggacacga gcaaccccga aagaacgctc
      781 ggggggcttc cgtccccgga agacagggcc gtcgtcctta atatcgatca tcatggtgac
      841 aataccaggt acggaacctt taactgggtg gacgaccggg cctcctcggt cggtttgatg
      901 atctacgaac tttttcttac ggcacggtgg gacctcacca ggagaacggc aggatcgctc
      961 ttcacggccg tcgccaccga taccggtttt ttccggttcc cctcgaccga cgagaggacc
     1021 ttgaggatcg cctcggacct ggttgcaaag ggagccgaac ctcacagggt ttaccaggag
     1081 gtttatgaaa accgttctct cgaaggtatc catctttggg gcagggggct ttcaagggcc
     1141 tcccttcacc tggacggcag ggtctgcgtc acctacctgt cgagagagga cctgtcctcc
     1201 ctgggggcat cgagggatga cacggagaac ctggtcaacg cgcttctttc cgtcaagaga
     1261 gtgaccgttg cagccctttt ccaggaggac gagggagcct gccgcgtcag catccgtact
     1321 aaggagcccg ttaacgcgag gaagatagcg gcgctgtggg gcggcggcgg tcatgagcgg
     1381 gcgtcggggt gcaagatgga gggtaacccg gaaaggtcac tcgaggcctt cgtcacgagg
     1441 ctgggggatc tggatgagat cgggatttct gatcattgac aaggccccgg gcaccaggag
     1501 ttcacaatgt gtggccctgg tgaagagggc cttgggcggg aaagagcggg tcggccacgg
     1561 gggaaccctg gaccagccgg cttcaggtgt cctcgccttg ctgatcggga gagccacgag
     1621 ggcatcccgt ttcgttatga tgctgccgaa gatctacctc gccgtggccc gtctcgggct
     1681 gaggacggat actgacgacc tttccggagc ggtgatatca gaggaagata ccggtctagt
     1741 tagtgaggaa agtatcgaca gggccctttt ctccctttac gggcaccggg atcagacccc
     1801 gccggccttc tcagccgtca aggtcaaggg caggagagcc catgccatgg caagggaagg
     1861 cctggatgtg ccgctttccg cgcggccggc gctggtcacc tctatcggga gaaccgggcc
     1921 cataatggca gggcgggacg tacccctgag gatcgtctgc cggcagggca cctacgtcag
     1981 gagcatcgta agggatctcg gggaaaccct gggatgcggc gcctctgttt taagcctccg
     2041 gagagaatcc ctcggacctt tcgccgctcc cgacgcactg cccagcggcg accttgccac
     2101 ggcggacgct tccaccctcg aggggaggat cctgcccttt tcatccctct ccggaagttt
     2161 tacttactac caggcttccc tcgccgatga gggaacgatc gcttcaggca gccccctgtc
     2221 gttggggaaa ctggcccgaa gaggctgggg cggacacggg accggagggt acatcctgat
     2281 cttcggaaag gccatggtat cctttgcccg cgtcgtacat gaagacggcg tatacctggt
     2341 caggcccgtc accaacatca ttctggaagg accgtaaacg atgatagccg tagtcgggtc
     2401 ctttgacggt ttccaccttg ggcacaggaa gctcttcgag gccgcggaaa acctatcgcg
     2461 ggtcatgtcc gattcctggt gcgtcgtgac ctttttccct caccctcagc ttgtcttggg
     2521 gaataaaacc tttttgcccc tttttaccga gccggaaaag gacatcctcg gtaaatgcct
     2581 ggggataccc gagatcgtca ggctggactt ttcaaaggac ctggccggga tggaacctga
     2641 tgacttcctg gggttcctgg aggacaggct tttcatcagg ggtcttgtcg taggggagga
     2701 cttccgtttc ggcaggggcc ggcggggcgg cgctgaactt ttgaaagagc tagcctcccg
     2761 gcgagggtgg gaagtcgaga tcgttcccca tctggctgcc ccttccggaa gaaagatcgg
     2821 gagcagcttc ataagggaga gcgtcatcag gggtgacctg ccccaggcct cggcggacct
     2881 cgggcacccc ttcatgatca ggggtcttat cgtccgtggt gacggaaggg gcagaacgat
     2941 cggtttcccg acgataaacc tttcgctccc ggggatgaaa gttttacccg ccagaggcgt
     3001 ctacgccggg ggggtcgctt tcgggaagga tgcactcccc gctgccgtca acatcgggct
     3061 gaaccccact ttccccggcg gggaagggat caggtgcgaa gcctatatcc ccgggttcag
     3121 gggcgaactc tacggaagag aggtttcgct tttccttttc aggaggctca ggagggaaaa
     3181 ggccttcccc gccgtctccc tgcttgtgga gcagatgaag atcgacgtga aggatacctt
     3241 ggctcaatgg cagggaacgt gcgaagggac gaaaagattc atggcctgtt gcggactgtg
     3301 aggcccgaag tgtgcccccc aggtccccgg gcggcattga agttgtgaag atcccatggt
     3361 aatattacgg tcgttcaacg acgggcctgt agctcagagg atagagcaac ggcctcctaa
     3421 gccgtaggtc agg
//
LOCUS       DAOSRG010000112         3427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24790_length_3427_cov_10.505931, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000112 DAOSRG010000000
VERSION     DAOSRG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24790_length_3427_cov_10.505931"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="rplR"
                     /locus_tag="PLM30_04670"
     CDS             complement(<1..362)
                     /gene="rplR"
                     /locus_tag="PLM30_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR54495.1"
                     /translation="MKKRTRNDMRLIRHRRLRKTLSGTGERPRLAVFRSLNHMYAQVI
                     DDTKGHTLAAASTMEKSVRNGSEGGGDIEAAKAVGRTVAERALEKGITQVLFDRGGHL
                     YHGKVKALADAAREAGLK"
     gene            complement(383..919)
                     /gene="rplF"
                     /locus_tag="PLM30_04675"
     CDS             complement(383..919)
                     /gene="rplF"
                     /locus_tag="PLM30_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR54496.1"
                     /translation="MSRIGRKLIDLPSGVTLSVDGEKVKVKGPKGELSVNLVPGIDVT
                     VEDNAVKVTRQGDDKRTRAYHGMMRALVNNLVQGVSAGFERRLEIVGIGWRAQMQGST
                     LVMNLGFSHPVEYAPPKGVEVTTDGPTKITVKGIDKQAVGQAAAVIRGFRPPEPYKGK
                     GIRYVGEHVIRKAGKAGA"
     gene            complement(932..1336)
                     /gene="rpsH"
                     /locus_tag="PLM30_04680"
     CDS             complement(932..1336)
                     /gene="rpsH"
                     /locus_tag="PLM30_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR54497.1"
                     /translation="MYVNDPIGDMLTRIRNANMVYHETVDMPLSKVKLAIARIFKEEG
                     YVKNFKVVNDPKKPYATLRLFLSYGPQRERVIQGLRRISKPGRRIYVQKDELPSVMKG
                     LGTAIISTSKGMMPEAEARKQGLGGEVICFIW"
     gene            complement(1366..1551)
                     /locus_tag="PLM30_04685"
     CDS             complement(1366..1551)
                     /locus_tag="PLM30_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOR54498.1"
                     /translation="MARKAMRIKAARDPKFKVRSYTRCPLCGRVHAYMRQFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(1569..2108)
                     /gene="rplE"
                     /locus_tag="PLM30_04690"
     CDS             complement(1569..2108)
                     /gene="rplE"
                     /locus_tag="PLM30_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR54499.1"
                     /translation="MPRLADLYKKDITGRLQKEFGYSNVMEVPRIQKVVVNIGVGEAK
                     TDYKYMESSIAELRTITGQQPMMKRAKKSVAGFKLREGMPVACSVTLRGARMWEFLDR
                     LISLALPRIKDFQGLSPKGFDGHGNYNLGLREQLIFPEIDYDKVSRIRGMNISIVSTA
                     KTDEEAMSLLQALGMPFAR"
     gene            complement(2127..2453)
                     /gene="rplX"
                     /locus_tag="PLM30_04695"
     CDS             complement(2127..2453)
                     /gene="rplX"
                     /locus_tag="PLM30_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR54500.1"
                     /translation="MSKIRIRKGDRVKVISGKDKGKEGKVLRCFPSRDLLLVEGVNVV
                     TKHVKPSAKNPQSGIVKQEAPIYSSKVQLVCTACGAATRVGRAFLEDGRKVRVCKKCG
                     EIIDKV"
     gene            complement(2468..2836)
                     /gene="rplN"
                     /locus_tag="PLM30_04700"
     CDS             complement(2468..2836)
                     /gene="rplN"
                     /locus_tag="PLM30_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR54501.1"
                     /translation="MIQLRTVMKVADNSGAKKIMCIQVLGGSRKRYASIGDIVVASVK
                     EAIPNSNACKGDVVKAVIVRTKKEVRRKDGSYVRFDDNAAVLIDNNGDPRGTRIFGPV
                     ARELREKRFMRIVSLAPEVV"
     gene            complement(2840..3130)
                     /gene="rpsQ"
                     /locus_tag="PLM30_04705"
     CDS             complement(2840..3130)
                     /gene="rpsQ"
                     /locus_tag="PLM30_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOR54502.1"
                     /translation="MNGRTISRKTQVGTVVSDRMTKTVVVKVDRMAKHALYGKPVLRS
                     KKFMAHDEEETCRIGDRIKIEETRPLSRHKRWKVVEIIERAPVLGGSQDKEA"
     gene            complement(3144..3350)
                     /gene="rpmC"
                     /locus_tag="PLM30_04710"
     CDS             complement(3144..3350)
                     /gene="rpmC"
                     /locus_tag="PLM30_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR54503.1"
                     /translation="MNARELRELSLEELSDKHKQFKEELFNLRFQHAIGQLGNTGRIG
                     EVKRSIARVLTVMREKEIGLKRPV"
BASE COUNT          570 a         1128 c          925 g          804 t
ORIGIN      
        1 aacttcaggc cggcttcgcg ggccgcgtcg gccagggcct tgaccttgcc atggtagagg
       61 tgaccgccgc gatcgaaaag gacctgggtg atgcccttct ccagggccct ctcggcgacc
      121 gtcctgccga ccgccttggc ggcctcgata tcgccgccgc cttcagagcc gttcctgacg
      181 cttttctcca tcgtcgaggc ggcggccagc gtatgcccct tggtatcgtc aatgacctgg
      241 gcgtacatgt ggttcaggct acggaatacg gcaaggcgcg ggcgttcccc ggtacccgaa
      301 agggtcttgc ggagacggcg gtgcctgatc aaacgcatat cgttcctggt tctctttttc
      361 atcctgccct tcacctcgct agctaggctc cggccttgcc ggctttacgg ataacgtgct
      421 cgccgacgta cctgatcccc ttgcccttgt agggctccgg cggtctgaaa cccctgatga
      481 ccgccgccgc ctgcccgacg gcttgcttgt ctatcccctt cacggtgatc ttggtggggc
      541 cgtcggtggt gacctccacc cccttggggg gggcgtactc caccgggtgg gagaagccca
      601 ggttcatgac cagggtgctg ccctgcatct gggcacgcca gccgatccct acgatctcga
      661 gccgcctttc aaaaccggcg ctgaccccct ggactaggtt gttgaccagc gccctcatca
      721 tcccgtgata ggccctcgtc ctcttgtcat cgccctgccg ggtgaccttg acggcgttgt
      781 cctcaacggt gacatctatc cccgggacga ggttcaccga gagttcgccc ttggggcctt
      841 tgaccttgac cttttcgcca tcgaccgaaa gggtcactcc ggagggaagg tctatcagtt
      901 tcctgccgat cctcgacacc gatgacaccc cctaccagat aaaacagatg acttccccgc
      961 caaggccctg tttgcgggcc tcggcttcgg gcatcatgcc cttggaggtc gatatgatgg
     1021 cggtaccgag ccccttcatc acgctgggga gctcgtcctt ctggacatag atcctgcgac
     1081 ccggcttgct gatcctcctg aggccctgga tcacgcgctc cctctgcgga ccgtaggaga
     1141 ggaacagcct tagggtcgcg tagggctttt tggggtcgtt gaccaccttg aaattcttga
     1201 cataaccctc ttccttgaag atcctggcga tggccagttt gaccttgctt aagggcatgt
     1261 ccaccgtttc atggtagacc atgttggcgt tccggatgcg cgtgagcatg tcccctatag
     1321 ggtcattcac atacattgca atagcctcct tgaccgcatc ccttgctacc agctggattt
     1381 gaccacaccg gggatcttgc cttcccgggc cagcttccgg aagcaacaac ggcacatatt
     1441 gaattgcctc atgtaggcat ggacacgacc acagagggga cacctggtgt aggacctcac
     1501 cttgaacttg ggatccctcg cggccttgat cctcatggct tttcgggcca ttcgcttcat
     1561 ctcctccctt accgggcgaa aggcattcca agagcctgga gcaggctcat agcctcttca
     1621 tccgtcttcg ccgtggatac gatggatatg ttcatccccc tgatacggct caccttgtcg
     1681 tagtcgatct cggggaagat cagctgctcc ctcaggccga ggttgtagtt cccatggccg
     1741 tcaaaaccct tgggcgacaa cccctggaag tccttgatcc tgggcagcgc cagggagatg
     1801 agcctgtcga ggaactccca catcctcgcc ccccggagcg tgacggaaca ggcgacgggc
     1861 atcccctcac ggagcttgaa gcccgccacg gacttcttcg ccctcttcat catgggctgc
     1921 tgccccgtaa tggtgcgaag ttccgcgatg gacgactcca tgtacttgta gtccgtcttg
     1981 gcctccccca ccccgatgtt cacgaccacc ttctggatcc gggggacttc catgacgttg
     2041 gaataaccga actccttctg aagccgtccc gtgatatctt ttttgtaaag atctgccaga
     2101 cgcggcattg tccagtcgcc tccccgttac accttatcga tgatctcgcc gcacttcttg
     2161 cagacacgga ccttgcgccc gtcctcgagg aaggccctcc cgacgcgggt cgcggcgccg
     2221 caggccgtgc agacgagctg gaccttgctg gagtaaatgg gggcctcctg cttgacgatc
     2281 ccgctctgcg ggttcttggc ggagggtttg acatgcttcg tcacgacgtt gaccccctcc
     2341 accagcagca ggtccctgga agggaagcag cgaaggacct taccttcttt ccccttgtcc
     2401 ttcccggaga tgaccttaac ccggtctcct ttgcgtatcc tgatcttgct catcgcgcca
     2461 cttcctccta gaccacttcc ggagcgaggg atacgatcct catgaaccgc ttttccctga
     2521 gctcgcgggc cacggggccg aagatcctcg tgcccctcgg gtcaccgttg ttgtcgatga
     2581 gaacggccgc gttatcgtcg aaccggacgt aggaaccgtc cttgcggcga acctcttttt
     2641 tggtcctgac gatcacggcc ttgaccacat cgcccttgca ggcgttgctg ttgggtatgg
     2701 cctccttgac ggaggcgacc actatgtcgc cgatggaggc gtaacgcttc ctgctccccc
     2761 cgaggacctg gatgcacatg atctttttcg cgcctgagtt atcggcgacc ttcatcaccg
     2821 tacggagctg gatcatccgt tacgcctcct tatcctggct gccgccgagg accggggccc
     2881 tctcgatgat ctcgacaacc ttccagcgct tatggcggct caaggggcga gtctcctcga
     2941 tctttatcct gtccccgatg cggcaggtct cctcttcgtc gtgggccatg aatttcttgc
     3001 tcctcaaaac gggctttccg taaagggcat gcttggccat ccggtcgacc ttgaccacga
     3061 cggtcttcgt catccggtcg ctcacgacgg tacctacctg cgtctttctt gagatggttc
     3121 taccgttcat cccttgttcc cccctacacc gggcgcttga gcccgatctc cttctcccgc
     3181 atgacggtga gcaccctggc gatggatctt ttgacctctc cgatgcgccc ggtgttcccc
     3241 aactgcccta tggcgtgctg gaaacggagg ttgaaaagtt cttccttgaa ctgcttatgc
     3301 ttgtcgctca attcctctag ggacaactcg cgaagttccc tggcgttcat cctcagacac
     3361 cacccattcc atcccgcgtt accaggcgga cctttatcgg cagcttgtga gaagccgtga
     3421 ggaaggc
//
LOCUS       DAOSRG010000113         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24837_length_3422_cov_10.042174, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000113 DAOSRG010000000
VERSION     DAOSRG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24837_length_3422_cov_10.042174"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLM30_04715"
     CDS             <1..1029
                     /locus_tag="PLM30_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HOR54504.1"
                     /translation="REVPDSRVLCLDTLSFALPIVRGVYTRSYLEMVRHMPHLWGYFY
                     ETTDDLQTRNGVIATLGELTEKLNLQGLKKALSTFSPDVIIFTHFFGAAAIAESFAPG
                     VPVCYVNTDFLSHVFHRNPSFSAWFVSSDETLRQYLADGLPPDRVFPTGIPVDPVYTE
                     PPGKEESRRRLGLAADDRVALVMGGGIGVGPIEEVVASLVGGGFATEVICGINEKVRE
                     TILERFAGRSGLRVHGFVDDMPYRYASADIIFMKPGGLSTSEALCVGTPIVITDPIPG
                     QEQRNSDFLLDRGAARTLFDYRLAAGKAESLLSATDERDRLRQAAAGIARPLAGRDIV
                     REVLKLEG"
     gene            1199..2185
                     /locus_tag="PLM30_04720"
     CDS             1199..2185
                     /locus_tag="PLM30_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOR54505.1"
                     /translation="MLIVKIDPGITDVLLASRFLQGRVRRTPTERSEPLSRLTGGEVF
                     LKWENQQLCGSFKIRGALNRMYSLTAEERARGVVTASSGNHAQGVALAARALSTRAVI
                     CVPGVCPETKREAIRRLGGDMVELKVTGHFYDDAEEAAHRLQEEEGMTYISSYADPFI
                     ISGAGTLGLEMIMDEPELDMVIVPAGGGGLINGVAIAVKALSPETQIWGVQSVASNPF
                     VVSWPGGKVLEVEYQDSLADGLTGLIPQSLLDLAKKRVTGFVDVEERDIARAIAFLHS
                     SHHQVVEGSGAVGVAAMASGRVAVKGLRVGVVISGGNIDHPRLMEILEEYSR"
     gene            complement(2237..>3422)
                     /locus_tag="PLM30_04725"
     CDS             complement(2237..>3422)
                     /locus_tag="PLM30_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOR54506.1"
                     /translation="GRSPSIVLAGLHLSDLDFTARGDGTRMALEKLKGYAGQGALTAT
                     GTADLLPGGVELGFSLEGSGLDLARVTKELKRNGSPALSGTADVTLSGSLKGGVWTGR
                     GELHSERVRAFGLDFDEVSLPLTLEEGGVRAENASARFCEGSLDGGGDLETSTGRWNI
                     RAALRGADMERVVEAVTDIDGRITGKGDLDLTLSGAFGKHLLVTGGGHFTATEGEVSG
                     FEALKAISSTYGMSALRYGRIDANFKVDGNVVTLLPGSRATAQTDDTFYRYLTVDGAA
                     GPGGNLNLYCSGLVNVQALNTLLGAIQGLAVSGTASPGAILEGLIGGIVGGMGKQDFR
                     DTSFRVEGTWSNPLFTNFRVAPPRDASRPPQDSNGTGQTLQDDRGHKVTISIPTGEST
                     GE"
BASE COUNT          578 a         1108 c         1131 g          605 t
ORIGIN      
        1 cgggaggtgc ccgactcgcg ggtcctctgc ctggacacac tctccttcgc cctgcctata
       61 gtgaggggcg tctataccag gtcctacctc gagatggtga ggcacatgcc ccacctctgg
      121 ggttactttt acgagaccac cgacgacctc cagacgagga acggtgttat agccaccctg
      181 ggggaactga cggaaaaact gaacctccag gggctcaaaa aggccctctc caccttttca
      241 cctgatgtca tcatcttcac ccacttcttc ggggccgccg ccatagccga gtccttcgcc
      301 ccgggtgtac ccgtctgcta cgtcaacacc gacttcctga gccacgtctt ccaccgaaac
      361 ccctcttttt cggcctggtt tgtctccagc gacgagaccc tccgccagta cctggccgac
      421 ggccttcccc ccgacagggt cttcccgacg ggtatccccg tggaccccgt ctacaccgag
      481 cccccgggga aggaagagtc ccgccggcgc cttggcctgg ccgccgacga cagggtcgcc
      541 ctggtaatgg gaggggggat aggggtaggc cccatcgagg aagtggtggc ctccctcgtg
      601 gggggcggct tcgccacgga ggtcatctgc ggcataaacg agaaggtccg ggagaccatc
      661 ctggaaaggt tcgccggccg gtcgggtctg cgggtccatg gtttcgtgga tgacatgccc
      721 taccgctacg cgtcggcgga catcatcttc atgaaaccgg gcgggctctc cacctcggaa
      781 gccctctgcg tgggtacacc catagtgatc accgacccca taccggggca ggagcagcgc
      841 aacagcgatt tcctgctcga tcgtggggca gcgaggaccc tcttcgacta ccggctcgcc
      901 gccgggaagg ccgagagcct cctttcggcc acggacgaga gggaccgcct ccggcaggcg
      961 gcggcaggga tcgcccggcc gctggcgggg agggacatcg tcagggaggt gctgaagctc
     1021 gaaggttagc gaagatcaaa ggttccatcc tcttattttt ccttttcgtc gccctcccgc
     1081 accgccggaa cccctccttg accgcggcag gccgccgggg cgccgcgacg gatccaactc
     1141 gcgcctgaag ggtaagtccc ttttcgtgta tcatcgatcc agataagtct tttaggaggt
     1201 gttgatcgtg aagatagatc ccgggataac cgacgtcctt ctggcctccc gcttcctcca
     1261 ggggagggtc cgccgcaccc cgacggagcg gtcggagccc ttgagcaggc tcacgggggg
     1321 cgaggttttc ctgaaatggg agaaccagca actctgcggc tctttcaaga tccggggggc
     1381 gctgaacagg atgtacagcc tcaccgccga ggagagggcc cggggggttg tgacggcctc
     1441 cagcgggaac cacgcccagg gcgtcgccct ggcggccagg gcactttcga cccgggcggt
     1501 gatatgcgtg cccggtgtat gtcccgagac gaagcgggag gccatcagga ggctgggcgg
     1561 cgacatggtg gaactcaagg tcacgggcca tttctatgac gacgccgagg aggcggccca
     1621 ccgtctccag gaagaggaag gcatgaccta catatcatcc tatgccgacc ccttcattat
     1681 cagcggtgcc gggaccctgg ggctggagat gatcatggac gagccggaac tggacatggt
     1741 catcgtgccg gccggtggcg gcgggctgat aaacggtgta gctatcgccg taaaggccct
     1801 cagccccgaa acccagatat ggggggtcca gtcggtggca tccaacccct tcgtggtctc
     1861 ctggcccggc ggcaaggtcc tcgaggtgga ataccaggac tccctggccg acggcctcac
     1921 ggggctgata ccccagagcc tcctggacct ggcgaaaaaa agggtgacgg gcttcgtgga
     1981 cgtcgaggag agggacatcg cgagggccat agctttcctc cattccagcc atcaccaggt
     2041 ggtcgaaggt tcgggggccg tgggtgtggc ggccatggcg agcggcaggg tcgctgtaaa
     2101 gggtttgcgg gtgggcgtgg tcatctccgg cggcaatatc gatcatcccc gcctcatgga
     2161 gatcctggaa gaatactccc gttgaaggga ccgatgtttc acgtgaaaca ggggcggccc
     2221 gggccgcccc tgttttttat tccccggtgc tctccccggt cgggatgctg atggtcacct
     2281 tgtgtcccct gtcgtcctgc aaggtctggc ctgtgccgtt ggagtcctgt gggggccggg
     2341 aggcatcccg gggcggtgcc accctgaagt tggtgaagag ggggttggac cacgttcctt
     2401 ccacccggaa gctggtgtcc cggaagtcct gcttccccat gccccccacg attccgccga
     2461 tgaggccttc caggatggcc ccgggggagg ctgtccccga cacggccagg ccctggatgg
     2521 ccccgagcag ggtgttcagg gcctgcacgt tgacgagccc cgaacagtag aggttgaggt
     2581 tgccgccggg gccggccgcc ccgtcgacgg tgaggtagcg ataaaaagtg tcgtcggtct
     2641 gggcggtggc ccggctgccg gggaggaggg tcaccacgtt gccgtcgacc ttgaagttgg
     2701 cgtcgatcct cccgtagcga agggcggaca tgccgtaggt agaggaaatt gccttgaggg
     2761 cctcgaaacc cgacacctcc ccctcggtgg cggtgaagtg cccgccgccc gtcacgagga
     2821 ggtgcttccc gaaggccccc gaaagggtca ggtcgaggtc gcccttaccg gtgatcctgc
     2881 catcgatgtc cgtgacggcc tcgacgaccc tctccatgtc ggcgccccgg agggcggccc
     2941 tgatgttcca ccggcccgtg gacgtctcga ggtcgccgcc gccatcgagg ctcccctcgc
     3001 agaaccgcgc cgaggcgttc tcggccctca cgcccccctc ctcgagtgtc agggggagcg
     3061 agacttcatc aaagtcgagg ccgaaggccc tcaccctttc ggaatgtagt tctcccctgc
     3121 cagtccagac accgcccttc agggaacccg agagggtcac gtccgccgtg cctgacaggg
     3181 ccggagaccc gttgcgcttg agttctttcg tgacccgggc gaggtcgagg ccggaacctt
     3241 ccaggctgaa gcccagctcc acgccgccgg gcaggaggtc cgccgtcccc gtcgccgtca
     3301 gggcgccctg gccggcgtaa cccttcagtt tctccagggc catgcgagta ccgtcgcccc
     3361 tggcggtgaa gtcgaggtcc gacaggtgga gaccggcgag gacgatggag ggggagcgcc
     3421 cc
//
LOCUS       DAOSRG010000114         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24838_length_3422_cov_7.418176, whole genome shotgun sequence.
ACCESSION   DAOSRG010000114 DAOSRG010000000
VERSION     DAOSRG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24838_length_3422_cov_7.418176"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLM30_04730"
     CDS             complement(<1..249)
                     /locus_tag="PLM30_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAS-barrel domain-containing protein"
                     /protein_id="HOR54507.1"
                     /translation="MLPKEYRTISDLSGPLLVVEKTENVRYDELVEIELASGERRRGR
                     VLEITRENALVQVFEGTSGIDLETTKVVFMGKVLMLPVS"
     gene            complement(239..2032)
                     /locus_tag="PLM30_04735"
     CDS             complement(239..2032)
                     /locus_tag="PLM30_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR54508.1"
                     /translation="MANKEKIVGSIAKISGPLVIADGMSGAGMYEVVRVGNLGLVGEI
                     IELKGAMASIQVYEETSGLKPGEPVESSGAPLSVELGPGLIEQFYDGVQRPLNLIEQA
                     ANSPYISRGINVPALDRSKKWDFVPLVEEGAQVSEGDLLGEVQETVLVRHLILAPHGV
                     KGSVKSISKGSFNVEETVAVIQDGEKEVSVAMMQRWPVRSPRPVRQRLAPNVPLPTGQ
                     RVVDTFFPLSRGGAACVPGPFGSGKTVIQHQLAKWADAEIVVYVGCGERGNEMTDVLL
                     EFPELVDPRSGEPLMKRTVLIANTSNMPVAAREASIYTAVTIAEYYRDMGYSVALMAD
                     STSRWAEALREISGRLEEMPGEEGYPAYLGTRLASFYERAGRAICLGSEGREGSITSI
                     GAVSPPGGDLSEPVSQNSLRVTKVFWGLDANLAYQRHFPAINWLLSYSLYAETLGEYW
                     DNLYDGEWSALRTEAMSILEEEDRLREIVRLVGIDALSRQERMILETAKSLREDFLHQ
                     NAYHEVDTYASMDKQFKMLRNIIHFHHLCQNALERGSTIQEINALPVRDQIARMRYED
                     EKQLNRIDELLSVMKEEVDKLLSEGGMGDVA"
     gene            complement(2072..2398)
                     /locus_tag="PLM30_04740"
     CDS             complement(2072..2398)
                     /locus_tag="PLM30_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOR54509.1"
                     /translation="MSDGKNFLMAAVGDFESVLPFQAVGVTPFSPENGLRELLLSLAR
                     EQYAVVFLVDSLYKDNREMVEELNENYTMSIIPVPGIRGSTGIGVSTIRESVERAVGM
                     DIFSVK"
     gene            complement(2391..3401)
                     /locus_tag="PLM30_04745"
     CDS             complement(2391..3401)
                     /locus_tag="PLM30_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOR54510.1"
                     /translation="MTDPTDYGYAVSRLRAMENRLLDQGVFQRLLDSEDLSSALKVMS
                     ETTYGKWLLEQQSAEKYELAIESELKYVFAETEHFVPDPCLYRICRLPYDIHNIKVLL
                     KGLFVQKQGGQRRLDLLTGLGNILVDDLVVAIESEDYRLMPFGFHRTVPECVSTWDQT
                     HDILQVERILDQALFSLQLKMADEVAFEGVKLFARSRVDAENLRNIARLKRMGFDSSQ
                     AAPFLHNGGFVPMEKIQALINEPFEGWERFLSFAEVSRTLSGAQEHADLDSLIVDLEK
                     SIDDFLSWVLAKYKYSPFAPENVLHYLWMKEIEAKNVRILLIGIGNGAERTVLRRLLR
                     NV"
BASE COUNT          718 a         1002 c          885 g          817 t
ORIGIN      
        1 gctgacgggt agcatcagga ccttccccat gaaaacaact ttggtcgtct caagatcaat
       61 gccggatgtc ccttcaaaga cctggaccag agcgttttct ctcgtgattt ccagcaccct
      121 gccccttctc cgttctccgc tggccagctc tatttcgacc agctcatcgt aacggacgtt
      181 ctcggttttc tcgacgacga gaagaggacc ggaaaggtca gatatggtcc tgtattcctt
      241 aggcaacatc tcccataccc ccttccgaga gaagtttatc gacttcttct ttcatcaccg
      301 atagtagttc gtcgatgcgg ttcagttgct tttcatcctc gtacctcatc cttgctatct
      361 gatccctgac gggaagggca ttgatttcct ggatcgtaga acccctctcc agggcgttct
      421 ggcaaagatg gtggaaatga atgatgttcc tcagcatctt gaattgcttg tccatggagg
      481 cataagtatc gacctcatgg taggcattct ggtgcaggaa atcctccctg agagacttgg
      541 cggtttccag gatcatcctc tcctgtctcg atagagcgtc tatgcccacg agcctgacga
      601 tctcccggag cctgtcctct tcttcgagga ttgacatggc ctcggtcctc agggcgctcc
      661 attccccgtc atagaggtta tcccagtatt ccccgagcgt ctcggcatag agggaataac
      721 ttagtagcca gtttatggcg gggaaatgcc tctggtaggc caggttggca tcgagacccc
      781 aaaaaacctt ggtcaccctg agggagttct ggcttaccgg ttcggacagg tcccctcccg
      841 gaggggaaac agccccgatg gacgtgatgg agccttccct gccttcggac ccgaggcaga
      901 tggcccttcc ggctctttcg taaaaactgg ccagccttgt ccctaggtaa gcggggtacc
      961 cttcctcgcc cggcatctcc tccagcctcc cggagatctc acgaagggct tcggcccatc
     1021 ggctcgtcga atcggccatg agggccaccg agtaacccat gtcgcggtag tattccgcga
     1081 tagtgaccgc cgtgtatatg ctggcctctc tcgctgcgac gggcatgttg gaggtgttgg
     1141 cgatcaggac cgtccttttc atcaacggct cccctgacct ggggtcgacc agttcgggga
     1201 attcaaggag cacgtcggtc atttcattcc cccgctcgcc gcatccgacg tacaccacga
     1261 tctcggcgtc tgcccatttg gccagctggt gctggatgac ggtcttgccc gaaccgaagg
     1321 gtccgggtac acaggccgct ccacccctgg aaagggggaa gaaagtgtcc accacacgct
     1381 gccccgtcgg aagtgggaca ttgggggcaa gtctttgacg gaccggtcta ggcgaccgga
     1441 caggccatct ttgcatcatg gccacggaga cctctttttc tccgtcctgt atcaccgcga
     1501 ccgtctcttc gacgttgaag gatcccttgc ttatgctttt gacgctaccc ttgacgccat
     1561 ggggggccag gatcaggtgc ctgaccagta ccgtttcctg gacctcaccg aggaggtccc
     1621 cttcagacac ctgggctcct tcctcaacaa gaggcacgaa gtcccacttt ttcgacctgt
     1681 cgagggcagg gacgttgatg cccctggaaa tataagggct gttggccgcc tgttcgataa
     1741 ggttcagggg cctctggaca ccatcataga attgttcgat gaggccgggg cccagctcca
     1801 cgctcagggg agcaccggag ctctcgacgg gctcacccgg tttcaaccct gaagtttcct
     1861 cgtagacctg tatggaggcc atcgctccct tcagctcgat gatctcgccg acaagtccga
     1921 ggttcccaac cctaacaact tcgtacattc cggccccgct cattccatcg gcaattacca
     1981 ggggaccgga gatcttggcg atggaaccta cgatcttctc tttgttggcc aataatatcc
     2041 cctccgagta cactcgtgtc ttcaggtgcg attacttaac ggaaaaaata tccatcccga
     2101 ccgctctttc aacactttca cggatcgtgc ttactcctat tccggtcgag cctctgatgc
     2161 cgggcacggg aataatgctc atggtgtagt tctcgttcaa ttcctccacc atctcccggt
     2221 tatccttgta aagggaatcg accaggaaaa ccacggcgta ctgctccctg gccaggctca
     2281 gaagaagctc tctgagcccg ttttcgggtg aaaagggggt cacgccgacg gcctgaaaag
     2341 gcaggacact ttcaaaatcg cccacagcgg ccattaaaaa atttttccca tcagacattt
     2401 ctcaaaagcc tcctaaggac cgtgcgttct gctccgttcc ctatgcctat gaggaggatc
     2461 ctaacattct tggcttcgat ctccttcatc catagataat ggaggacatt ctccggcgcg
     2521 aagggactgt acttgtactt cgcgaggacc caggacagaa aatcatcaat ggatttctcg
     2581 aggtccacga taagagaatc gaggtccgca tgttcctgcg ccccggaaag ggtccttgag
     2641 acttcggcaa aggagaggaa tctttcccaa ccttcgaagg gttcattgat caatgcctgg
     2701 attttctcca tgggtacgaa tccgccgtta tgcaggaacg gcgcagcctg agacgaatcg
     2761 aaacccatcc ttttgagtct tgcaatattc cgcaggttct ccgcatcgac ccttgaccgc
     2821 gcgaacagtt tcaccccttc aaaggcgact tcgtcggcca tcttgagttg gagtgagaag
     2881 agcgcctggt cgagaatacg ctcaacctgg aggatgtcgt gggtctgatc ccaggtcgaa
     2941 acgcactccg ggacagtcct atggaatccg aagggcataa ggcgataatc ttcgctctct
     3001 atggccacta ccaggtcatc cacgaggatg ttcccgagac cggtcaagag atccagacgt
     3061 ctctgcccgc cctgtttctg aacgaaaagg cctttcaaga ggacctttat gttgtgaatg
     3121 tcgtagggaa gtctgcagat ccgatatagg caagggtcgg ggacgaaatg ctccgtttcg
     3181 gcaaagacat acttgagctc cgattcaata gcaagctcat atttttctgc gctctgctgc
     3241 tccaggagcc atttcccata ggtcgtctcc gacataacct tgagcgcgga ggacaggtct
     3301 tcgctgtcca gtagtcgttg aaagaccccc tggtcaagta gccggttctc catcgctctg
     3361 agtcttgaga cggcgtaacc ataatcggta gggtcggtca ttgtgggagc ctccctaaca
     3421 gt
//
LOCUS       DAOSRG010000115         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_24936_length_3410_cov_9.375261, whole genome shotgun sequence.
ACCESSION   DAOSRG010000115 DAOSRG010000000
VERSION     DAOSRG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24936_length_3410_cov_9.375261"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="PLM30_04750"
     CDS             <1..135
                     /locus_tag="PLM30_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR54511.1"
                     /translation="DRSLDVHISNLRRKIGTYPDGSERIKTIRSLGYILALPPEDFKP
                     "
     gene            132..1550
                     /locus_tag="PLM30_04755"
     CDS             132..1550
                     /locus_tag="PLM30_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54512.1"
                     /translation="MRERKLFLRIFLWFLGAMLLMALVSVLLTVFMTQQGIILTGQQE
                     ALSNALDRNGKRLLELHETEAPSRLSEEIERIRNETGLAIFIFDGRGTPVPIEGGPSG
                     EFLVRLGKDALNLLDQGTTFQTRGQFSSVFRRLVSPSGKDYILVGSFRRSPYIARVLT
                     GNPKALAIHLLGLLVTALVFCFLLARYLADPLVRLSRTARSVSGGRLDTPVDDGVKKR
                     SDEIGELARSFEAMTRHLTTLLEAQRRLIQDISHELRTPLARLGVALELARKKSGAVA
                     KESLDRIGRESEVLNVMIGDLLSLSRLEAEMEHMDYEKADLGSILESVVEDANFEARA
                     MKRKVELVYPDDLGLVRLYNELVRRAVENIVRNGLRYAPEGTSVIVRAEPLDRDGRPG
                     VSISVRDRGPGVPEAELEEIFKPFYRTEASRSRDTGGTGLGLAIARKAALAHGGDIRA
                     VLPPGGGLLVEMWLPINPQGRV"
     gene            1651..2553
                     /locus_tag="PLM30_04760"
     CDS             1651..2553
                     /locus_tag="PLM30_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOR54513.1"
                     /translation="MTPPVLAALVAVYFFWGTTYLGMKFAIETLPPFIMLGLRFTVAG
                     AIMFAWEWVRLRPRITLGQWRGMGMVGALMLLGGTGGVAWSEQVVPSNIAAVVVATVP
                     LWIAVIRWVFLRHDRPERVTIVGLLTGFAGIIMLVVYTSGPSAAEGYGLGLVVLGFST
                     FLWSAGSLYSRAADQPDSPALGISMQMLVGGLLNLIVGILCNGGSMVPLAEVSMRSAV
                     ALAYLVVFGSIVGFSSYIWLLKKTDPALASSYAYVNPVVAIILGWLLAGERLNTGSLA
                     ATAVILFGVILIVRGTVRPAARSS"
     gene            complement(2638..3399)
                     /locus_tag="PLM30_04765"
     CDS             complement(2638..3399)
                     /locus_tag="PLM30_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOR54514.1"
                     /translation="MRDWDRRLHLSEKQRRDMERYYDDLYNRRWERERRYRDLEKDYL
                     KRNWDRDSFRDIDRYRRDLLQRRQESLTPPRDSRDRFRSILDDGQRKTFDSLIRERDR
                     ERPTPPKFQDRPAPPKFQEKPTPPKFQDRPTPPKFQEKPTPPKFQEKPTPPKFQEKPA
                     PPKFQEKPAPPKFQEKPAPPKFQEKPAPPKFQEKPAPPKVPNKPAPPKFQEKPAPPKI
                     PNKPVPPVLTKNPGPAPGQKKPLPPVVKKPVKDGK"
BASE COUNT          526 a         1046 c         1102 g          736 t
ORIGIN      
        1 gacaggagcc tcgatgtcca tatcagcaac ctccgccgga agatcggcac ctaccccgac
       61 gggtccgagc gcatcaaaac catccggagc ctgggctaca tcctcgcttt gccgccggag
      121 gacttcaagc cgtgagggag cggaagcttt tcctgaggat attcctatgg tttctggggg
      181 ccatgctttt gatggccctg gtgagcgtcc tcctgacggt cttcatgacc cagcagggca
      241 tcatcctcac cgggcagcag gaagccctct cgaacgccct ggacaggaac gggaaaaggc
      301 ttctggagct ccatgagacg gaggccccct cccgcctgag tgaagagatc gaaaggatca
      361 ggaatgaaac gggcctcgcc atattcatct tcgacgggcg ggggacgccg gtgcccatcg
      421 aaggcggccc atcgggggaa ttcctggtga ggctcggcaa ggatgccctg aacctcctcg
      481 accaggggac cactttccag accagggggc agttctcctc ggtcttcagg cggctggttt
      541 ccccctccgg caaggactac atcctggtcg ggtccttccg gaggtcgccc tacatcgcga
      601 gggtccttac gggaaacccc aaggcgctgg cgatccacct cctggggctc ctcgtcacag
      661 ctctcgtgtt ttgcttcctc ctcgcccggt accttgcgga tcccctggtc cggctgagca
      721 ggaccgcccg gtccgtctcg gggggacgcc tggacacccc ggtggacgac ggggtgaaga
      781 aacgctccga cgagataggg gaactggccc gcagcttcga ggccatgacc aggcatctga
      841 cgaccctgct cgaagcccag cggcggctca tccaggacat ttcccatgaa ctgcgcaccc
      901 ccctcgcccg gcttggtgtg gctcttgaac tggcgaggaa aaagtccggg gccgtggcga
      961 aggagtccct ggacaggata ggacgtgaat cggaggtact gaacgtcatg ataggggacc
     1021 tgctctccct gtcgaggctc gaggcggaga tggagcacat ggactatgag aaggccgatc
     1081 tcgggtccat cctcgaatcc gtcgtcgagg acgccaactt cgaggcgagg gccatgaaac
     1141 gcaaggtgga gctcgtctac cccgacgacc tggggctggt gaggctttac aacgaactgg
     1201 tccgaagggc cgtggagaac atcgttcgca acggtctccg gtacgccccc gaagggacct
     1261 ccgtcatagt cagggcggaa cctctggaca gggatggccg cccgggggtc tccatctcgg
     1321 tccgcgaccg ggggcccggg gttcctgaag cggaactgga ggagatcttc aaacccttct
     1381 acaggaccga ggcatcccgc tcccgtgata cgggcggtac cggcctcggc ctcgccatcg
     1441 cacggaaggc ggccctcgcc cacgggggcg atatccgtgc cgtgctgccc ccgggaggcg
     1501 gtcttctggt ggagatgtgg ctccctatca acccgcaggg ccgggtatga ccgccgcccc
     1561 tttagcaatg atctcttcag tgtgtataat cgaaagcgct gaggcagtcc aagttcacaa
     1621 aaggtgagat gacccttgaa ggacgatcgc atgacgcccc cggtcctggc cgccctggta
     1681 gcggtttatt ttttttgggg gaccacgtac ctggggatga aattcgccat cgagacgctc
     1741 ccccccttca tcatgctcgg cctccggttc accgtggcgg gagcgatcat gttcgcctgg
     1801 gaatgggtgc gactcaggcc caggataacc ttgggccagt ggaggggcat ggggatggtc
     1861 ggtgccctca tgctcctggg cgggaccggt ggcgtcgcct ggtcggaaca ggtggtgcct
     1921 tccaacatcg ccgctgtcgt ggtggccacg gtgccgctct ggatagccgt tatccgctgg
     1981 gtcttcctcc gccacgaccg ccccgagagg gtgaccatcg tggggctgct gacgggtttc
     2041 gccgggataa tcatgctggt ggtctatacc agcggcccct ccgcggccga gggttacggc
     2101 ctggggctcg tcgtcctcgg tttttcgacc ttcctgtggt cggcgggttc actatattcg
     2161 agggcggccg atcagcccga ctccccggcc ctgggcatct cgatgcagat gctcgtcggg
     2221 ggccttctta acctcatcgt ggggatcctg tgcaacggcg gttccatggt gcccctggca
     2281 gaggtgtcta tgaggtccgc cgtggcactg gcctacctgg tcgtgttcgg gtcgatcgtg
     2341 ggtttcagtt cctatatctg gctcctgaag aagaccgacc cggccctggc ctccagctac
     2401 gcctacgtga acccagtggt ggcgataatc ctcgggtggc tcctagccgg cgagaggctc
     2461 aacacgggga gccttgccgc gacggcggtc atcctgttcg gcgtgatcct tatcgtgagg
     2521 gggaccgtga ggccggccgc ccggtcgtcc tgacaccctg ggcctcgccg ggaagtgaca
     2581 aaaaagggag ggttcacccc caagggtgag ccctccctct tgttactggt gaggaactta
     2641 ttttccgtcc ttcaccggtt tcttcacgac cggtggaagc ggttttttct gtcccggggc
     2701 gggcccgggg ttcttcgtca gtacaggcgg caccggcttg ttcggtatct tcggcggtgc
     2761 cggtttttcc tggaactttg gcggcgccgg tttgttcggt acctttggcg gtgccggttt
     2821 ttcctggaac ttcggcggtg ccggtttttc ctggaacttt ggtggtgccg gtttttcctg
     2881 gaacttgggt ggtgccggtt tttcctggaa cttgggtggc gccggttttt cctggaactt
     2941 tggcggtgtc ggtttttcct ggaacttggg tggcgtcggc ttttcctgga acttgggtgg
     3001 cgtcggcctg tcctggaact tcggtggcgt cggcttttcc tggaactttg gcggtgccgg
     3061 cctgtcctgg aacttcggcg gcgtcggcct ttcgcggtcc ctctcccgga tcagggaatc
     3121 gaaggtcttc cgctgcccgt cgtcgagtat cgaccggaac ctgtccctcg agtcgcgggg
     3181 cggtgtcagg ctttcctggc gcctttggag gaggtcccgc ctgtaccggt cgatatcgcg
     3241 gaaggaatcg cggtcccagt tcctcttcaa gtaatccttc tcgaggtccc tgtagcgcct
     3301 ctccctctcc caccggcggt tgtagaggtc gtcgtagtag cgctccatgt cccttctctg
     3361 cttttcgctc aggtggagcc tccggtccca atccctcatc gcttcccggt
//
LOCUS       DAOSRG010000116         3393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25114_length_3393_cov_9.127621, whole genome shotgun sequence.
ACCESSION   DAOSRG010000116 DAOSRG010000000
VERSION     DAOSRG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25114_length_3393_cov_9.127621"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="selB"
                     /locus_tag="PLM30_04770"
     CDS             complement(<1..1352)
                     /gene="selB"
                     /locus_tag="PLM30_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOR54515.1"
                     /translation="MMEKTEVSIVLGTAGHIDHGKTTLVRALSGVDCDRLRDEKRRGI
                     TIELGFAPLTLPSGKTISIVDVPGHEKFIRQMVAGAVGVDGIILVVASDEGVRPQTRE
                     HLEILGLIGVEHGLIALTKADLVEEEFLELAIEEVKEEVRGTFLEGAEVIPVSSVTGF
                     NLDVLLESLEKLVAGISPRGSEGPFFMPIDRAFPVKGFGAVVTGTAYRGRVRKDDVLS
                     VLPRGIKTDIRSVQVHDNPVDEARSGQRVALSLGGVSLSDLKRGDVLCTPGIFVPSGR
                     LDVDLKILASSPDPLRHWQRVRIHLGTTDTVGRIALLGGDRIEPGERAFAQVVTETPV
                     VAVRGEPFIIRFYSPLTTIGGGKVLMSVASRPKGPRGRKGHQELLDKVSRAKDPGELL
                     LAVTLDQLVVTVDEASVLMQEQPGVVVSLAGELDRAGRLLLVDSGEGLIVNGDMARKK
                     L"
     gene            complement(1336..2751)
                     /gene="selA"
                     /locus_tag="PLM30_04775"
     CDS             complement(1336..2751)
                     /gene="selA"
                     /locus_tag="PLM30_04775"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOR54516.1"
                     /translation="MREEIKSMLKDLPPMDELLQKPWTSRYQEVLGREAVRSTFADVI
                     GEVRRDLLKGLIHSFDTTLIESETRARLEKRSRKSLRRVVNATGVVIHTNLGRSCLGN
                     EVLENVRAVSASYSNLEYDLEEGARGQRHSHVEWLLSSVTGADAAMVVNNNAGAVLLC
                     LAALGRGNEVIVSRGELVEIGGSFRIPDIMDFSGAILREVGTTNRTHLPDYLKAINTQ
                     TAMILKVHPSNFRVVGFHSEVSREELAALAREKELIFMEDLGSGVVADLSPWGLQGEP
                     TVRQCVESGVDLVTFSGDKLLGGPQIGAIVGRSQLVERIRHYPLLRAMRVDKMTLAAF
                     EVTLRLYLEGRTGEIPTLRMISQPGKTLRKRARNLASMIRRVTGLQAREVVTQDAVGG
                     GAYPAAVLEGFGVEVEPPEGVSDGSALEWLRSAPVPVIASAKEGKILLHVRTMLDGDG
                     MDVIGALKDLSPREAGHDGED"
     gene            complement(2990..>3393)
                     /locus_tag="PLM30_04780"
     CDS             complement(2990..>3393)
                     /locus_tag="PLM30_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase"
                     /protein_id="HOR54517.1"
                     /translation="RRWCRHNVAYWSGAPFLGIGPSAWGYDGTVRTGNVADLEHYRLM
                     IEKGVRPTLFSERLEGKRRAFELSALALRTRWGIRLQEYRRLWGDQMLKGLQDSLEGL
                     PPRLFRKAPGRIALSKQGMRVGNAIWERLVP"
BASE COUNT          649 a         1118 c          914 g          712 t
ORIGIN      
        1 agttttttcc ttgccatatc gccgttgact atcaaccctt ctcccgagtc aaccaggagc
       61 aaccgtccgg ccctatccag ctcaccggct agagagacga caacccctgg ttgttcctgc
      121 atcaggacag aagcctcgtc gacggtgacc accagttggt ccaaagtgac cgccaggagg
      181 agctcgccgg ggtctttcgc cctggagacc ttgtccagca gttcttggtg accctttcgg
      241 ccccgtggcc ccttcggcct actggcgacc gacatcagca ccttcccgcc gccgatcgtg
      301 gtcaaggggc tgtaaaagcg gatgatgaag ggttcccccc tgacggccac gaccggcgtt
      361 tcggtcacga cctgggcgaa ggccctctcg ccgggttcga tcctgtctcc acccagcagg
      421 gcgatcctgc ccaccgtatc ggtggtcccg agatggatcc ggaccctctg ccagtggcgc
      481 agaggatccg gagaagaagc cagtatcttg agatcgacgt caagcctgcc tgaagggacg
      541 aatattcccg gggtacatag gacgtcgccc cttttcaggt ccgaaagcga aaccccgcca
      601 aggctcaagg cgactctctg gccgcttcgg gcctcatcga ccgggttgtc atggacttgg
      661 accgaacgaa tatccgtttt gataccccgg ggcaggaccg acagtacgtc gtccttcctt
      721 accctacccc ggtaagcggt accggtgacg acggcaccga aacccttcac aggaaaagcc
      781 ctgtcaatgg gcatgaaaaa ggggccttcg gaaccccttg gggatatccc cgccacaagc
      841 ttttccagtg attctaggag gacgtccagg ttgaaccccg tcaccgatga gacaggtatc
      901 acttcagccc cctcgaggaa ggtccccctg acctcttcct tcacttcctc tatggccagt
      961 tccaggaatt cttcttccac aagatcggcc ttcgttaagg ctatcagtcc gtgctcgacc
     1021 cctattaacc cgaggatctc caggtgttcc cttgtctggg gcctcactcc ctcgtcggag
     1081 gccacgacca ggatgatgcc gtcaaccccg actgcgccgg cgaccatctg ccttatgaac
     1141 ttctcatgac cggggacatc gacaatacta atagttttgc cgctgggaag tgtgaggggg
     1201 gcgaacccca gttcgatggt gatcccacgc cgtttctcgt ccctcaacct gtcgcagtcg
     1261 accccgctga gcgcccgtac cagggtggtc ttaccgtgat cgatatggcc ggcggtaccg
     1321 agcacgatgg agacctcagt cttctccatc atggccggcc tccctgggcg acaggtcttt
     1381 tagcgccccg atcacatcca tcccgtcccc gtcaagcatg gtcctgacat ggaggaggat
     1441 cttcccctct tttgccgacg cgatcacggg gacaggggca cttctaagcc attcaagggc
     1501 cgaaccgtca gaaactcctt ccggtggttc cacctcaacc ccgaaaccct cgaggaccgc
     1561 agcagggtaa gctcccccgc cgaccgcatc ctgggtcaca acctcccttg cctgcaaacc
     1621 ggtcactcgt ctgatcatgg aggccaggtt gcgggccctt ttcctcaagg ttttccccgg
     1681 ctgggagatc attctcaatg tggggatctc gcccgttctg ccttccaagt aaagtctcag
     1741 ggtcacctca aaagcggcca gggtcatctt gtcgaccctc atcgccctca gcaaggggta
     1801 atgccggatc ctctccacca gctggcttct cccgacgata gcgcctatct gggggccgcc
     1861 gagcagcttg tcgccggaga aggtcaccag gtccacccct gactcgacgc attgtctgac
     1921 cgtcggttcg ccctggagtc cccagggtga taggtccgcc accaccccgc tccccaggtc
     1981 ctccatgaag atgagttcct tctcccgggc cagggccgcc agttcctccc tcgagacttc
     2041 cgaatgaaaa cccaccaccc tgaagttgga aggatgcacc ttgaggatca tggcggtctg
     2101 agtgtttatg gctttgagat aatcggggag gtgtgtcctg ttggtcgtcc cgacctctct
     2161 taagatggcc ccggaaaaat ccataatgtc ggggatcctg aaggaacctc ctatctccac
     2221 cagttctccc ctggagacga tgacctcatt gccccgcccc aaagcagcca ggcagagcag
     2281 gacggcgcca gcgttgttat ttacgaccat ggccgcgtcg gctccggtga cgcttgacag
     2341 gagccattcg acatgggagt gcctttgccc cctggccccc tcttcgaggt catattccag
     2401 gttgctgtaa gaagccgaaa ccgccctcac attttccagg acctcattcc caaggcagga
     2461 ccttcccagg ttcgtgtgga tgacgacccc ggtggcgttc accactctcc tgaggctctt
     2521 tctggagcgt ttttccaacc gggccctggt ctccgattcg atgagggtgg tgtcgaagga
     2581 atggatgagc cccttgagca ggtccctcct gacctcgccg atcacatcgg cgaaggtcga
     2641 tctgacggct tcgcgcccga gaacttcctg gtaccggctt gtccacggtt tctgaagtag
     2701 ttcgtccata gggggcaggt ccttaagcat cgacttgatc tcttcccgca tcatcgcacc
     2761 ttccctcaag ggggatcggt cacgccctgg accgagcccc gtattcactg aacctggcag
     2821 gatcatacca aagcggtgga caggggtttt acctcgccct tcatatcagt ataacgggtg
     2881 aaaggtagcc tgggtgcatc cgatgatggg aagcggtctc cgagagggca cccgcgacaa
     2941 gagctatcgg agaagacacg ccccgcccgg cacaggcctc tcgagttcgt catggtacaa
     3001 gcctttccca gatggcattc cccaccctca tgccctgctt gctcagggcg atccttccgg
     3061 gcgcttttct gaaaagacgc gggggtagac cttccaggga atcttgaagg cccttcagca
     3121 tttgatcgcc ccataggcgt cggtactcct ggagcctgat gccccagcgg gtcctcaggg
     3181 ccagggccga caactcgaag gcccgacgtt tgccttccag cctctctgaa aacagggtcg
     3241 gccttacccc cttttctatc atcaaacggt agtgctccag gtccgctacg ttacctgtcc
     3301 ttacggttcc gtcgtacccc caggccgaag gcccgatccc aaggaagggg gcaccggacc
     3361 agtaggccac gttatgcctg caccaccgac ggg
//
LOCUS       DAOSRG010000117         3368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25366_length_3368_cov_6.645337, whole genome shotgun sequence.
ACCESSION   DAOSRG010000117 DAOSRG010000000
VERSION     DAOSRG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25366_length_3368_cov_6.645337"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1264)
                     /locus_tag="PLM30_04785"
     CDS             complement(59..1264)
                     /locus_tag="PLM30_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOR54518.1"
                     /translation="MDPLLSTVASRVSPREIAELASKLIRIPSHWDVPSKEEEVVKAV
                     ASFLTIENIPFTLQPVEGSRNNIIATIKGEGGGRSLALSGHLDTVPPYNMEVEPFVAK
                     IEGGRIYGRGAVDMKGPVAAMLMTLAAFRRSGVPLKGDLIFAGVLAEETNSDGSETLI
                     RSGFSADGAIVGEPSDREYAIGHRGLEWIEVEFFGKTAHGGVPQDGVNAIVHAAKFIN
                     RVQEKIVPKLALREHPHMGPSVMNFGRIEGGTQPSTVADHCVVEMDRRYISSESLQEV
                     LAEYQEVLDELSREDPTFRAELKLMDLGKMAHFYHAPLETSPDDPIVKAVVDSIREVS
                     GIEPRMTTRRGWTDAAIFGHFGKIPTVVFGPGSLSRSHGKAEYITLDELEEGFRTYVS
                     IAARFCGAA"
     gene            complement(1329..2744)
                     /locus_tag="PLM30_04790"
     CDS             complement(1329..2744)
                     /locus_tag="PLM30_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HOR54519.1"
                     /translation="MSVEAGSEKKKRVKVPHTYVILISVVVLATVMTYILPAGVYDRF
                     KDERTGRTLVDALSYHHVDRTPVSIFKMFESVPKGMKETAEIIFFIFICGGAFSIIQA
                     TGAIDGAIGKAVLGLKGKEKLMIPITLLIFSIGGATYGMAEEVIVFIPIGVALARAVG
                     YDDIVGVAILSTGANIGFSGGTLNPFTVGVAQSIAELPLFSGLGLRFAGHVMLFVIAC
                     WYIMRYAAKIKADPTQSIIYDLAESDPTRHTVTPDLPFTGIHKLVMLVILASFAYMIW
                     GVMTQGFFIMELSTVFIAMGVIGGLVGRLAPSKMASAFVAGAKDIAFGALVVGIARAI
                     LVVMSQGQIVDTVIHALASWVAMLPGAMTAVGMFWVHVIINFFIPSGSGQAAATMPIM
                     TPLADLVNITRQTAVLAFQYGDGFTNQIIPTSAALMGVLGMAKMPYERWFKFIWPLMV
                     FWIAAGSVMVFIAAVIEYGPF"
     gene            complement(2929..>3368)
                     /locus_tag="PLM30_04795"
     CDS             complement(2929..>3368)
                     /locus_tag="PLM30_04795"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HOR54520.1"
                     /translation="GGALIFALFAGAMGSIGPFPMRLDGSVLSALRTFSLAYFLAVMG
                     IQAGPDIPRILAEHGFALVAIGAASAMASLLAGLLVGRCFFKLNWVIFLGAIPGAMTS
                     TPGLGAAIESTGSDECSAGYGATYPTAIFCMVLFTKLIIQALL"
BASE COUNT          668 a         1041 c         1020 g          639 t
ORIGIN      
        1 agaccgggtc gacgacggtc ccgttcctca caacgatatc catgtccgtg cacctccttc
       61 atgccgcccc gcagaaacgg gcggcgatgg agacgtaggt cctgaatcct tcttccagct
      121 cgtcgagcgt aatgtactcc gccttgccgt gcgacctgga caggctcccc gggccgaaca
      181 ccacggtggg gatcttgccg aagtggccga agatggccgc gtcggtccac cccctcctcg
      241 tggtcatcct gggttcaata ccactgacct cccttatgga gtcaacgacg gccttaacga
      301 tggggtcatc gggcgaggtc tccaggggtg cgtggtagaa atgggccatc ttgcccaggt
      361 ccatcagttt cagttctgca cgaaaggtcg ggtcctcccg cgacagttcg tccaggacct
      421 cctggtactc ggcgaggact tcctggagcg attccgatga tatgtacctc ctgtccatct
      481 cgactacgca atgatcggcc acggtgctcg gctgggtccc tccctcgatg cggccgaagt
      541 tcatgaccga cggccccatg tgggggtgtt ccctcaaggc cagtttcggg acgatcttct
      601 cctggacgcg gttgatgaat ttcgcagcgt ggacgatggc gttaaccccg tcctggggga
      661 ccccgccgtg ggcggtcttc ccgaagaact ccacctcgat ccattcgagt ccccggtggc
      721 cgatggcata ctcccggtcg gagggttcac caacgatggc gccgtcggca ctgaaaccgg
      781 acctgatgag ggtctcgctc ccatcgctgt tggtctcctc ggccaggacc ccggcgaaga
      841 tgagatcacc cttgaggggg acccccgacc ggcgaaaggc cgcgagggtc atcagcattg
      901 ccgcgacggg ccccttcatg tccacggcgc cgcgaccgta gatcctcccc ccttcgatct
      961 tcgccacgaa gggttcgact tccatgttgt agggcgggac cgtgtcgagg tgccccgaaa
     1021 gggccaggct cctgcctccc ccctcgccct tgatggtagc gattatatta ttcctggaac
     1081 cttccacagg ctgaagcgtg aaagggatat tttctatggt caggaaggag gccacggctt
     1141 tcactacttc ttcttccttg gagggcacat cccagtggct gggtatccgg atcagcttcg
     1201 aggccagttc cgctatctcc cggggggata cccttgacgc caccgtcgaa agcaagggat
     1261 ccatcaggac cacctcattt cctggaattc ggggagggac tcggaagccc ctccccgttc
     1321 agttttccct agaaaggtcc gtactcgata acggcggcga tgaacaccat caccgacccc
     1381 gcggcgatcc agaacaccat gagaggccaa atgaacttga accatcgctc gtagggcatc
     1441 ttggccatgc cgaggacacc catgagggcc gccgaggtag gtatgatctg gttcgtgaaa
     1501 ccatcgccgt actggaaggc gaggaccgcg gtctgcctgg tgatattcac caggtcggcc
     1561 aggggtgtca tgataggcat ggtggcagcg gcctgcccgc tccccgaggg tatgaagaag
     1621 tttattatca cgtggaccca gaacatgccc acggcggtca tcgcccccgg gagcatggcg
     1681 acccaggaag ccagggcgtg gatcaccgtg tccacgatct gcccctggga catgaccacc
     1741 aggatggctc gggcgatacc cacgacaaga gctccaaagg ctatatcctt ggcgccggcg
     1801 acaaaggccg aagccatctt gctcggggcc aggcgaccca ccaggccacc gatcacaccc
     1861 atggcgatga aaaccgtgga gagctccatt atgaagaagc cctgggtcat caccccccag
     1921 atcatgtagg cgaagctggc caggatcacg agcatgacga gcttgtggat ccccgtgaag
     1981 gggaggtcgg gcgtaaccgt atggcgggtc gggtcggatt cagcgaggtc gtagatgatg
     2041 ctctgggtgg ggtcggcctt gatcttcgcc gcgtaacgca tgatgtacca gcaggcgatg
     2101 acgaagagca tcacgtggcc cgcgaagcgc aacccgagac cggagaagag gggaagctcg
     2161 gcgatgctct gggcgacccc caccgtgaag gggttcaggg tgcccccgga gaaaccgata
     2221 ttggcgccgg tcgataagat ggccaccccg acgatgtcgt cgtaaccgac ggccctcgcc
     2281 agggcgaccc cgatcgggat gaagacgata acctcctcgg ccatgccgta ggtggccccg
     2341 cctatggaga agatgaggag tgtgatgggg atcatcagct tctccttgcc cttgaggccg
     2401 aggacggctt taccgatggc gccgtcgatg gcccccgtgg cctggatgat ggagaaggcg
     2461 ccgccgcaaa tgaagatgaa gaagattatc tcggcggttt ccttcatccc cttggggacc
     2521 gattcgaaca tcttgaagat gctcaccggg gtcctatcga catgatgata ggacagtgca
     2581 tcgacgaggg tccgcccggt cctttcgtcc ttgaacctgt catagacccc tgcgggaagt
     2641 atgtaggtca ttaccgtcgc gaggacgacg acggagatca gtatgacgta ggtatgaggg
     2701 accttgactc gctttttctt ctcacttcct gcctccaccg acatcttgga acctcccttc
     2761 gagaaaagat caagtcttcc gggaaaatac ccgatcacat gccgcttctt ctgctcttct
     2821 cttcattcac ctcccggtcg ggctgcccgg tcccacggtc attcccggga ccggtcgact
     2881 tttgccactg tcattagccg gagttgcgag aaggcccttc acacctcctc acaggagagc
     2941 ctgtattatc aatttcgtga agaggaccat gcagaatatc gccgtcgggt aggtcgcccc
     3001 gtagccggcg gagcattcgt cggaccccgt cgattctatg gcggcgccca gccccggcgt
     3061 actggtcatg gcccccggta tggcaccgag gaatatcacc cagttgagtt tgaaaaaaca
     3121 cctccccacg aggagacccg cgaggaggct cgccatcgcc gaggccgccc cgatggccac
     3181 gagggcgaag ccgtgctccg ccagtatcct gggtatgtcc gggccggcct ggatgcccat
     3241 gacggcgagg aagtaggcca gcgaaaaggt ccggagcgcc gaaaggaccg aaccgtcaag
     3301 gcgcatgggg aaaggcccga tactgcccat ggccccggcg aagagagcga agatcagggc
     3361 gccgccgg
//
LOCUS       DAOSRG010000118         3348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25605_length_3348_cov_4.172183, whole genome shotgun sequence.
ACCESSION   DAOSRG010000118 DAOSRG010000000
VERSION     DAOSRG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25605_length_3348_cov_4.172183"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLM30_04800"
     CDS             complement(<1..301)
                     /locus_tag="PLM30_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR54521.1"
                     /translation="MERILLADDDAGLCELLTEFLESEGFKVDSVHDGAKVVSKALAE
                     RYSLVILDVMLPGLSGMEVLKEIRRQSAIPVLMLTARGDDVDRIVGLELGADDYLP"
     gene            complement(427..795)
                     /locus_tag="PLM30_04805"
     CDS             complement(427..795)
                     /locus_tag="PLM30_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOR54522.1"
                     /translation="MKREIRTDRSPDPIGPYSQGLAAGNRIYVSGQGPLNPATGEIPE
                     TIEEQTRQVLTNIRNILEAGGAAMEQVVKTTAHLADLEDFEAFNSVYREFFNEPYPVR
                     TTVGSELLGILVEIDVIAEL"
     gene            complement(912..2162)
                     /locus_tag="PLM30_04810"
     CDS             complement(912..2162)
                     /locus_tag="PLM30_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HOR54523.1"
                     /translation="MATIHNKSATPWKTDKWYTSPWNFIPEVREGMKFSPNIQFHDVS
                     LRDGEQQAGLLFNKDQKIALAEKLAELGIQRIEAGMPVVSEQDAQAIKEIVKRNLGPK
                     IFSFARCMKEDVKRSVDIGVKNIVMEVPASDHILKYAYRWEFEKAIEVSIEATLYAKE
                     NGLYVSFFTIDGTRADIDDYLKIVDRVASEGHMDELTVVDTFGGLNPHSVPYLIKKVK
                     EHIPDKPIGVHFHDDFGLGAATTIMGLASGADIAHTSISGIGERAGNAPYEDVAMALL
                     TMYGVDTGLRYEKIYPLSKYLREITGLTVRQNRGIVGDYVADIESGIVAAWYKNVEGI
                     APLELSPFLYDLVGHPDTKVVIGKLSGLPTVDIYLDKLGIQTDDKDAKAEILKRIKDT
                     ALEKRRLLTIEEFEEIAASVLDKP"
     gene            complement(2382..>3348)
                     /locus_tag="PLM30_04815"
     CDS             complement(2382..>3348)
                     /locus_tag="PLM30_04815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR54524.1"
                     /translation="TTELAEEVSRYIVDGKFDVIVGLGGGSILDVAKMSSALTANPGK
                     TRDYFGPGKVKNKGLPTIMIPTTAGTGSEVTKHAIFLDQETNVKKAVASSALLPDVAI
                     VDPMLTVTSPRNVTSASGFDAWLHAAEPFVSKNANPITDALSLQAVSMIAKHLGVAWS
                     DPNDLDARYHVSLGSLMSGMVLNNAGTSLVHALAYPIGGEYHATHGISLSVLLISCFK
                     AIAASKGERLVQIAGAMGENMDGLSTREGVEVALDAMAVFMKSVELPISLEEVGIIDR
                     SGVERWAVEGWNERRLLGRCARDLTVEDIAAIYRDAFEARTLGWD"
BASE COUNT          617 a         1037 c          962 g          732 t
ORIGIN      
        1 tgggcaggta atcatcggcc cccagttcga ggcccacgat gcggtccacg tcgtcacccc
       61 gggcggtgag catcaagacg gggatggcgg actgccgcct gatctccttc agaacctcca
      121 tccccgaaag cccggggagc atcacgtcca ggatgaccag cgagtaccgt tccgccagcg
      181 cctttgagac gaccttggcg ccgtcgtgga ctgagtcgac cttgaaaccc tccgattcga
      241 ggaactcggt gagcaactcg caaaggcccg catcgtcatc ggcaagcagg atccgttcca
      301 cggcttttcc ccctgactga gaagggtttc tccatattat cctatggagg ccttaaaagc
      361 cgtcaaggag ggtaagaact tgtaaaagga gcctttacat tcctttacag gcgaaatacc
      421 agcccttcac agttcagcga tgacgtcgat ctccactagg atccccagca gctcgctccc
      481 cacggtggtc ctcaccgggt agggttcgtt gaagaattcc ctgtaaacgc tgttgaaggc
      541 ctcgaaatcc tctaggtcgg caaggtgggc ggtggtcttc accacctgtt ccatggcggc
      601 gccaccggcc tcgaggatat tccttatgtt ggtcaggacc tgccgagttt gctcctcgat
      661 ggtctccgga atctcgcctg tcgcgggatt cagcgggccc tgccccgaca cgtagatcct
      721 gttgcccgcc gccaggccct gggaataggg ccctatcgga tcgggcgatc tatcggtgcg
      781 gatctccctt ttcatcgcgt tgacctcctc gactgagatc ttgaacgatc aaagaccttc
      841 cgcaaggccc ccgccgaaga gcggctccgc cgcccacggg cggccttccc gagcgagggc
      901 cggctttacc cctacggctt gtcgagcacc gacgcggcga tctcctcgaa ctcctcgatc
      961 gtcagaaggc gcctcttctc cagcgccgta tccttgatcc gtttcaggat ctccgccttg
     1021 gcgtccttat cgtcggtctg gatacccagt ttgtccaggt aaatatcgac ggtgggaaga
     1081 ccactcagct tcccgatgac caccttcgtg tcggggtgac ccaccaagtc gtagaggaag
     1141 ggcgaaagtt caaggggcgc aatcccctct acgttcttgt accaggccgc cactatcccc
     1201 gactcgatat cggctacgta atcccctacg atgcccctgt tctggcgcac ggtcagcccc
     1261 gtgatctccc tgagatattt cgagaggggg tagatcttct cgtagcggag gcccgtgtcg
     1321 acgccgtaca tcgtgaggag cgccatggct acatcttcgt agggcgcgtt gccggcccgc
     1381 tcaccgatgc ccgatatgga ggtgtgggcg atgtccgcgc ccgaagccag ccccatgatg
     1441 gtcgtggccg ccccgaggcc gaaatcgtcg tgaaagtgaa cgccgatggg cttatcgggt
     1501 atatgttcct tgaccttttt gatgaggtag gggacggaat gggggttcag cccgccgaag
     1561 gtgtccacca cggtgagttc gtccatgtga ccttcggagg cgacacggtc gactatcttc
     1621 agatagtcgt cgatatcggc cctggtgccg tcgatcgtga agaaggagac gtaaagcccg
     1681 ttctccttcg cataaagggt ggcctcgatg gacacctcga tggccttctc gaactcccac
     1741 cggtaggcgt acttcaggat atgatcgctg gcggggacct ccatgactat gttcttcacg
     1801 ccgatatcga cggacctttt cacgtcctcc ttcatgcacc tcgcgaagga gaagatctta
     1861 ggccccaggt tcctcttgac gatctccttg atggcctgag cgtcctgctc ggaaaccacg
     1921 ggcatgccgg cctcgatcct ctggatgccc aattcggcca gtttctcggc tagggctatc
     1981 ttctggtcct tgttgaagag gagccccgcc tgttgctcac cgtcgcggag agacacgtcg
     2041 tggaactgga tgttcggaga aaatttcatc ccctcccgta cctcggggat gaaattccag
     2101 gggctcgtgt accatttgtc cgtcttccat ggtgtcgcac tcttattgtg gatagttgcc
     2161 atcatcgttc ctcctctgta agacgactca ggactccctc aatcgaagga cgtcccgggc
     2221 aggtttcgtt tctcggggcg attaccgggt cccatcgcag ggaaaagtcc ggcgccgaca
     2281 tttcgctcgc cgaatgctta ccgcgcgccg gcagggctat cgacaccggg ctcctcgccc
     2341 gatgccgttt cccatcttac ttcccgacct tttcacgcat cctaatccca acccagggtc
     2401 ctagcctcaa aggcatccct gtatatggcg gctatgtctt caacggtaag atcgcgggcg
     2461 cagcggccca ggaggcgcct ttcgttccat ccttcaacgg cccatcgttc gacgccggac
     2521 cggtctatta tccctacttc ctcgagactg atggggagtt cgacgctctt catgaagacc
     2581 gccatggcat cgagggcgac ctcaacccct tcccgggtgg aaaggccgtc catattctcg
     2641 cccatcgcgc cggcgatctg gacgaggcgc tctcccttcg aggccgctat cgccttgaag
     2701 cacgagataa ggagcacgct gagggagatc ccgtgggtgg cgtggtactc accgcctatg
     2761 ggataggcca gggcatggac gaggctggtc ccggcgttgt tgaggaccat gcccgacatc
     2821 aaggaaccta gggacacgtg gtaacgggca tcgaggtcgt tggggtccga ccatgccacg
     2881 cccagatgct tcgcgatcat gctcaccgcc tgcagggaca gagcgtcggt gatggggttc
     2941 gcgtttttcg agacgaaggg ctcggcggca tggagccagg catcaaaccc cgaagcgctg
     3001 gtgacgttgc gcggtgaggt caccgtcagc atcgggtcga cgatggccac atcggggagc
     3061 agggctgaag aagctacggc cttttttacg ttggtctcct ggtcaaggaa gatggcgtgt
     3121 ttcgtaacct ccgacccggt ccctgcggtc gtggggatca ttatcgtggg gagccccttg
     3181 ttcttgacct tgccgggacc gaaataatcg cgggttttac cgggatttgc ggtcagcgcc
     3241 gaggacattt tcgccacatc gaggatgctg cccccgccta agcccactat cacgtcaaat
     3301 ttgccgtcga cgatgtacct cgacacttct tccgcaagct ccgtggtc
//
LOCUS       DAOSRG010000119         3347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25612_length_3347_cov_11.311968, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000119 DAOSRG010000000
VERSION     DAOSRG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25612_length_3347_cov_11.311968"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLM30_04820"
     CDS             complement(<1..262)
                     /locus_tag="PLM30_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54525.1"
                     /translation="MGKLRTTPAVFFALLLLACPAFAGQTYQFKGEVNGITRGALLFK
                     AFAEGLKPESMEMILDEEPDETGRVRRVFLDLFGCELGGVRIE"
     gene            389..1318
                     /locus_tag="PLM30_04825"
     CDS             389..1318
                     /locus_tag="PLM30_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR54526.1"
                     /translation="MDERKRVFLADASMFIIAVFWGSGFGVTSWLLGFFSPMWLMAVR
                     FTMSALVLLLLFKNRLKYLGRKDIFLGVALGFLLFLSFAAHIWGLVFSTPGKQSFISA
                     SNVVMVPFLFALFYRRWPSWVATAGAFITTAGLLVMAFTPGMTFNFGDFLSLVLALGI
                     ALHVLGVGNLSRRMDPVALTVVQLASTGFFFLATALVFEPPPHVASIDPRALLGLLYV
                     VVLVTVIPFLVQTVAQRFSPEVHAAILLSLEGPFGYVIAVLIGQEVLNFQIAAGGVII
                     LIGVALAELDTLIRRTFHLPGPEAIMSGGDPDA"
     gene            1311..2477
                     /locus_tag="PLM30_04830"
     CDS             1311..2477
                     /locus_tag="PLM30_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR54527.1"
                     /translation="MHRVKKLLPRMAGGSFAIMSFSYAYFFLPLYLDHLGLKSQATIG
                     WVLGAFYAASTLPRPFMALLVERFAFRATLTGAALCSAVGSIGLALSGLTLPFLILSR
                     VLMGFGFGLFLVSLTTYQTIAIPREVRGGAFALITIGAMAPFLCVVPLLDWLLQHGGY
                     GVYPWFGPLVALACALAGMTFAGVDRDEGMARRGAGWGTYSQLFHLTRARVMFLAVFI
                     YGMTDAAVISIPVLALSKGLLSSYFMVTFAAGAILTRLAGFRLMDAFPKDIVYTLMAA
                     WNAFSLFGTSFSSSPRAFAFWGLLYGIGVGVGFPLSLAMIGEAAPPGLHPKATAVVWF
                     ILDGCFFLTPVVVGYTSALLGVAGAFRVLPAILFFAAPAVYFFLWRPLGKKGHP"
     gene            complement(2511..2828)
                     /locus_tag="PLM30_04835"
     CDS             complement(2511..2828)
                     /locus_tag="PLM30_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54528.1"
                     /translation="MLKLEVEHYIQLGEKMRERAAELCRMYMKVHYDCDDCSVLVTNM
                     ETGQSGRFRIMFNESDAEEMLYLPPEYFWLTDDEVAVRMKIDRDIREGQLEERRKSRP
                     SAG"
BASE COUNT          515 a         1064 c          982 g          786 t
ORIGIN      
        1 gctcgatcct tacgccgccc agttcacacc cgaagaggtc caggaagacc cgccggaccc
       61 ttccggtctc gtcgggctcc tcgtccagta tcatctccat cgactcgggt ttcagcccct
      121 ccgcgaaggc cttgaagagg agggctcccc tggtgatgcc gttgacctcg cccttgaatt
      181 ggtaggtttg gcctgcgaag gccgggcagg cgaggaggag cagggcgaaa aacaccgccg
      241 gcgtggttct cagcttaccc attgggggac ccccaccctt catggagatg accgtgcccg
      301 aagatgatat catatccttc tttgtacaca accgcatggc tggtggtatg ctgtaaaggc
      361 aacgtcgtcg aggggcaggt gcgctttttt ggacgagagg aaaagggtct tcctggccga
      421 cgcctccatg ttcatcatcg ccgtgttctg ggggtccggt ttcggtgtga ccagctggct
      481 tttgggtttc ttctccccga tgtggctcat ggccgtaagg ttcacgatga gcgcgctggt
      541 ccttttgttg ctttttaaaa accggctgaa gtacctgggc aggaaggata tattcctggg
      601 cgtggccctg ggcttcctcc tgttcctttc cttcgcggcc cacatctggg ggctggtctt
      661 ttccaccccc gggaagcagt ccttcatctc cgcttccaac gtggtcatgg tgcccttcct
      721 cttcgccctg ttctacagga gatggccctc ctgggtcgcc acggccgggg ctttcattac
      781 gacggcgggg ctgctggtca tggccttcac cccggggatg accttcaact tcggcgattt
      841 cctctccctc gtcctggccc tgggcatagc cctccatgtc ctcggcgtgg ggaacctcag
      901 ccggaggatg gaccccgtgg ccctgacggt ggtccagctg gcctcgaccg ggtttttctt
      961 cctcgccaca gccctggtct ttgagccgcc cccccacgtc gcttccatag accccagggc
     1021 tttgctgggg ctcctttacg tcgtggtcct cgtgacggtg atccccttcc tcgtgcagac
     1081 cgtggcccag agattcagcc ccgaggtcca cgcggccata ttgctctccc tggagggtcc
     1141 cttcggttac gtcatcgccg tgctgatcgg ccaggaagtg ctgaacttcc agatagccgc
     1201 ggggggcgtt atcattctca tcggcgtcgc cctggcggag ctcgatacct tgataaggag
     1261 gaccttccac cttcccgggc cggaagccat catgtcgggg ggagaccccg atgcataggg
     1321 tgaaaaaact gctccccagg atggccggag gcagtttcgc catcatgagt ttcagttacg
     1381 cctatttttt cctccccctg tacctcgatc atctggggct gaagtcccag gcgacgatcg
     1441 gctgggtcct gggggccttc tacgcggcat cgaccctccc caggcccttc atggccctcc
     1501 tggtggagcg cttcgccttc agggccaccc tgaccggcgc cgccctgtgc agcgccgtcg
     1561 ggagcatagg gctggcactt tcggggctca ccctgccttt cctgatcctc tccagggtac
     1621 tcatgggctt cggcttcggt ctcttcctgg tttcgctcac cacttaccag actatagcca
     1681 tcccccgcga ggtgaggggc ggggccttcg ccctgatcac cataggcgcc atggcgccct
     1741 tcctctgcgt cgtgcccttg ctggactggc tgctccagca tggcggctac ggcgtctacc
     1801 cgtggttcgg gccactggtg gccctggctt gcgccctggc ggggatgacc ttcgccggcg
     1861 tggaccgcga cgagggcatg gcgcgaagag gggcggggtg ggggacctat tcgcagcttt
     1921 tccacctcac ccgggccagg gtgatgttcc tggcggtgtt catctacggg atgaccgacg
     1981 cggcggtgat atccatcccg gtcctcgcct tgagcaaggg ccttctgtcg tcctacttca
     2041 tggtgacctt cgcggcgggg gcgatcctga cgaggctggc ggggttcagg ctcatggacg
     2101 ccttccccaa ggatatcgtg tacaccctca tggcggcctg gaacgccttt tcgctcttcg
     2161 gcacgtcctt ttcgtcttct ccccgggcct tcgccttttg gggtcttctt tacggcatag
     2221 gggtgggggt gggtttcccc ttgtcgctgg cgatgatagg tgaggcggcg ccgccggggc
     2281 tgcaccccaa ggccaccgcc gtggtatggt tcatcctgga cgggtgtttc ttcctgacac
     2341 ccgtagtggt gggttacacc tcggcgctgc tgggggtggc cggcgccttc agggtcctgc
     2401 cggccatcct tttcttcgcg gccccggccg tctacttctt tctctggagg cccctgggga
     2461 aaaagggtca cccctaggtc tcccgccggg gcggtaggct cagccgcctc ctagcccgcc
     2521 gaggggcgcg acttcctgcg ctcctccagt tggccctcgc ggatatccct gtcgatcttc
     2581 atccggacgg caacctcgtc gtcggtgagc cagaagtatt cgggagggag atagagcatt
     2641 tcttccgcgt ccgattcgtt gaacatgatc cggaaccgcc cgctctgccc ggtctccatg
     2701 ttggtcacca ggaccgagca gtcgtcgcaa tcgtagtgga ccttcatgta catcctgcac
     2761 agttcggcgg cccgttccct catcttctcc ccaagttgga tgtagtgttc gacttcaagc
     2821 ttcagcactg tccccgacct cccctttatt cttacgggag gtagtataac atactatcct
     2881 gataaatcga tatctgggat tggaaaccgt gaatcgagaa tcgtaaatcg tgaatcgaaa
     2941 atcgtgaatc gtaaatcgag aatcgtggag tcaaggtaaa ggcaaaagcg aaaagcgtaa
     3001 acggtgaacg ggaaacggga aaagacgtga gtcgcaagag caaaacaatt tgtttacgaa
     3061 tttgaccttt cgatttgcga ctcgcggccc ttgcgactca cggcccttgc gatccccggc
     3121 ccttgcgatt cacggccctt gcgactcacg gtccttgcga ttcacggtcc ttgcgattca
     3181 cggcccttgc gattcacggt ccttgcgact cacggccctt gcgactcacg gcccttacga
     3241 ttcacggccc ttacgattca cggtccttgc gatccccgct ttatctgcct tcttctttct
     3301 tccatccttt ccagaagacc ggcatcggga ggaggggaag caggccg
//
LOCUS       DAOSRG010000120         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25628_length_3346_cov_9.115770, whole genome shotgun sequence.
ACCESSION   DAOSRG010000120 DAOSRG010000000
VERSION     DAOSRG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25628_length_3346_cov_9.115770"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /gene="dnaB"
                     /locus_tag="PLM30_04840"
     CDS             <1..1195
                     /gene="dnaB"
                     /locus_tag="PLM30_04840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR54529.1"
                     /translation="QVMREMAEKGKPVDPLTFLEDLSRRGLASALGGQGFIAGLVDSV
                     PTTANVEYHARIVRDKSIHRRLIQVGAEIARLGYSEDREIEDVMDQAERAVFEITQRG
                     SQVPFRKVGDVLGPTFRTIEEKFHSPDSSVTGVPSGFDDFERITGGFQPGSLNIIAAR
                     PSMGKTALALDIGMHASVDRGIPVLMFSLEMGAEQLVQRMLGSRARVDVHDLRTGMFH
                     ESAWEKLATAAGDLSRAPFFIDDSSVLTTLELRARSRRFKSQVMKTGNGLIVVDYLQL
                     MQLTRRTESKQQEVAEISRSLKAIARELDVPLIALSQLSRAVESRQEKRPQLSDLRDS
                     GAIEQDADLVILLYRPGYYDAPADEGDNTADVIVAKHRNGPTGTIRLTFFKEHARFAS
                     QAWSS"
     assembly_gap    1204..1303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1304..2498
                     /locus_tag="PLM30_04845"
     CDS             <1304..2498
                     /locus_tag="PLM30_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOR54530.1"
                     /translation="DISGRKNIAAAANLFLFAVNAWGIWNSTSRSAVFSYAVGLCVLA
                     LLSARSMGTRKALARASVILALLAATFFGTLLFGRGNVFIMKSVQMVEQFKTIGKRDS
                     IWLTTAEMFKMHPLGGVGLGHYKWNYLEAQRRMVAKHPDMKWQYTYWAHSEVFQWFCE
                     TGMFGGLALIALGGWWLKCFLSRLRRGDTLSPEGIWASSFLFLIWFDALWTRPFHRIE
                     DVLWLAFAFGIANRELLPSSFSWTEIRKDWVLRSLGLTMSTVAVTGLLFFARGMEGDR
                     FLRRASMAEDPMARVGLIEKAEGRLMVRDTAQRELAYQYIRMAVDEKRGELLGEGLMR
                     LHQHFRQEPHARDLGRLMGWYSDVREADLLESVARYLKPGAYSIVDGRLLLSSEDYQF
                     FFGSD"
     gene            complement(2531..3205)
                     /locus_tag="PLM30_04850"
     CDS             complement(2531..3205)
                     /locus_tag="PLM30_04850"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54531.1"
                     /translation="MAKMLESRIGETDVADAALDAIRNAALESGNIRFEGNCYTREWH
                     EEAKRRGLPVAHSTSEALALFLIPENRELLSGLDVFTDREIRGYYETRLEQYVKTLEI
                     EMNVMEDMIRTGVLPAVTKQIHLEASTLDLIAGRHGEGKASLWEEQIDQLVGIKEGLL
                     EGANRMTSLKDSLASLSQDEKARTLTERGVPILEGLRGMCDAAERMTSGEFWPYPRYN
                     ELLLPG"
BASE COUNT          634 a          949 c          991 g          672 t
ORIGIN      
        1 ccaggtcatg agggaaatgg ccgaaaaggg gaaacccgtg gaccccctca cctttctcga
       61 ggacctgtcc aggagagggc tcgcctcggc cctggggggt caaggcttca tagcggggct
      121 cgtggattcc gtccccacca cggccaatgt cgagtaccac gcaaggatcg tacgggacaa
      181 gtcgatccac cgcaggctga tccaggtcgg cgctgagata gcgcgcctgg gctactcgga
      241 ggacagggag atagaggatg tgatggacca ggctgaacgg gcggtcttcg agatcaccca
      301 gagagggagc caggttccct tcaggaaggt gggagatgtc ctcggcccca ccttcaggac
      361 catcgaggag aaattccact cgcccgacag ctctgtgaca ggtgtcccca gcggtttcga
      421 cgatttcgaa aggatcaccg gggggttcca gccggggagc ctcaacataa tagccgccag
      481 gccctccatg ggaaagactg ccctcgccct cgacatcggc atgcacgcct cggtggacag
      541 ggggatcccg gtcctgatgt tcagcctcga gatgggtgcg gaacagctgg tccagcgcat
      601 gctgggatcc agggccaggg tggatgtcca cgacctgcgg acggggatgt tccatgaaag
      661 cgcctgggaa aagctggcca ccgcggcagg cgacctttcg agggctccct ttttcatcga
      721 tgacagttcc gttctcacga cgctcgaact gcgcgcccgg tcaaggcggt tcaagtccca
      781 ggtcatgaaa accggcaacg gtttgatcgt ggtcgattac cttcagttga tgcagttgac
      841 caggagaacg gagagcaagc agcaggaggt cgccgagata tcccgttccc tcaaggccat
      901 agcaagggag ctcgatgtac cgttgatagc cctctcccaa ctctcaaggg cagtcgaatc
      961 aaggcaggaa aaaaggcccc agctttccga ccttcgtgat agcggcgcca tcgagcagga
     1021 cgccgacctg gtcatccttc tttatcgccc ggggtattac gacgcgcctg cggatgaggg
     1081 cgacaacacg gccgatgtga tcgtggcgaa gcacaggaac ggccccaccg gaaccataag
     1141 gctcaccttt ttcaaggagc acgcccgttt cgcgagccag gcctggtcgt cctgaatggg
     1201 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgatatc agcggcagga
     1321 agaatatcgc cgcggcggcc aaccttttcc tttttgccgt caacgcctgg ggcatatgga
     1381 attccacgag ccgttcggcc gttttttcct acgccgtcgg cctttgcgtt cttgcccttc
     1441 tctcggcccg gtccatgggg acaaggaagg cccttgcgcg ggcttctgtc atcctggcgc
     1501 tgctggccgc gacctttttc gggacccttc tcttcggtcg gggcaacgtc ttcatcatga
     1561 agagcgttca aatggtcgaa cagttcaaga ccatagggaa gagggacagc atttggctga
     1621 ccaccgccga gatgtttaaa atgcaccccc tgggcggtgt ggggcttggc cattacaagt
     1681 ggaactacct cgaagcccag aggagaatgg tcgcgaagca tcccgacatg aaatggcaat
     1741 acacctattg ggcccacagc gaggtctttc agtggttttg cgaaaccggg atgttcgggg
     1801 gcctggcact catcgccctc gggggctggt ggctcaaatg tttcctctcc aggctgagga
     1861 ggggggacac cctctcaccg gaagggatct gggcctcttc ttttttgttc ctgatctggt
     1921 tcgacgcgct ttggacacgc cccttccaca ggatcgagga tgtcctttgg ctggcctttg
     1981 ccttcggcat cgccaacagg gaactcctgc cctcgtcctt ttcctggacc gagatcagga
     2041 aagactgggt cctgaggagc ctggggttga ccatgagcac cgtcgccgtc acaggtttgc
     2101 tttttttcgc caggggcatg gagggagaca ggttcctgcg gagggcgtcc atggcggagg
     2161 accctatggc gcgggtggga ctcattgaaa aggcggaggg acggctgatg gtgagggata
     2221 ccgcgcagag ggagctggcc tatcaatata tcaggatggc cgtggatgag aagagggggg
     2281 agttgctcgg agaggggcta atgaggcttc atcaacattt cagacaagag ccccacgcca
     2341 gagacctcgg ccggttgatg gggtggtatt ccgatgtgag agaggccgac ttgcttgaaa
     2401 gtgtcgcacg gtacctgaag cccggggctt acagcatcgt ggacggccgg ttattgcttt
     2461 cttcggagga ttatcagttc tttttcggtt ccgattgaag caccctgggg tcagatcccc
     2521 tcgccacagc tcaaccagga agaaggagtt cattataccg ggggtagggc caaaactccc
     2581 cggaggtcat cctttcagcc gcgtcgcaca tgcccctcag gccctcgagg atcggcacgc
     2641 ccctctccgt cagcgttcgg gctttctcat cctggctgag cgacgccagg ctgtctttta
     2701 atgaggtcat cctgttggcg ccctccagga gcccttcctt gatgcccacc agttgatcga
     2761 tctgttcctc ccaaagggag gccttcccct ccccatgacg acccgctatc aaatccaggg
     2821 tcgaggcctc gaggtggatc tgcttcgtta ccgccggcag gacgcccgtc cgtatcatat
     2881 cctccatgac attcatctcg atctccaggg tcttcacata ttgttccagc ctcgtctcgt
     2941 aataaccccg gatctcgcga tcggtgaaaa catcgagccc cgaaagaagc tccctgttct
     3001 cagggatcaa aaaaagggca agggcttcac tggtgctgtg ggcgacggga agacccctcc
     3061 tcttggcctc ttcgtgccac tcccgggtgt aacagttgcc ctcgaaccgg atattaccac
     3121 tttcgagggc ggcgttcctg atggcgtcga gggccgcgtc cgccacatcg gtctcgccta
     3181 tcctggattc caacatcttg gccatgcgtt cgagccccca ggcccagata gaaaggatca
     3241 tcgtcatggg gcccgccggc gattgcgatg cgccgggcgc gcggaactcg aacttgttgc
     3301 ccgtgaaggc tatcggggcc gtgcggttcc tgtcggtgtt gtccat
//
LOCUS       DAOSRG010000121         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_25858_length_3323_cov_9.622705, whole genome shotgun sequence.
ACCESSION   DAOSRG010000121 DAOSRG010000000
VERSION     DAOSRG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25858_length_3323_cov_9.622705"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PLM30_04855"
     CDS             <1..1384
                     /locus_tag="PLM30_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOR54532.1"
                     /translation="GGLDAIYPFTTMNIRPPCNGEGTGSDNGGRYLGMSIQAAAASVV
                     FILTIMVIATGKMRRSVAALLAASLLMGLNIIPAQKAVSYIDFNTIGLLMGMMIIVGV
                     ISGTGLFQYIAVLTVKATGGRIHLTYIAVLAVTALLSAFLDNVTTVLLISPVVISLVD
                     LMDVNPFPFLIGEAFASNIGGTATLVGDPPNMIIGSFAHFSFMDFIVNLTPAVALVMV
                     IVTAWLLYHFRKDLEGGERATERIAKVDESKLIKDKGLMIRASTAMVLVVAGFVVHHI
                     LDLPASVIALLGATVLLAVVPVDGDEVIHRDIEWPTIMFFVSLFIIVGALNETGVITA
                     ASELFISFFKGRPLAAMVAMLWFSGLVCAFINNVAFTVTFVYVAQDLAASTGMAAAPL
                     FWALSLGACLGGNGTFLGAAANVVVADIAEKSGNRINFRTFMRTGLKVVFISLITASL
                     YIVIRWGGLF"
     gene            1458..1913
                     /locus_tag="PLM30_04860"
     CDS             1458..1913
                     /locus_tag="PLM30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR54533.1"
                     /translation="MLVGDLMDRDLTAVSPGTTIAEAVEIMAGQRVTGIPVVGDDGKV
                     VGFLSEKDIVKAALPGYFDLLRDSSFLPDYGQFQKRLQGISLERVDKYMQTQVIVLDE
                     QDTDLNAAMVLITKSLKRAPVVKDGVFSGVLSRSDLLDYILHSKKEPSD"
     gene            1913..2182
                     /locus_tag="PLM30_04865"
     CDS             1913..2182
                     /locus_tag="PLM30_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54534.1"
                     /translation="MDEKGVRIKFFGPWQRAAGTAELFLPLEEPVTASSLVERLAGVY
                     GERFPVREEGLICLYDGERGPKALDGGEEVPPGSTILFLGVIESG"
     gene            2385..2645
                     /locus_tag="PLM30_04870"
     CDS             2385..2645
                     /locus_tag="PLM30_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOR54535.1"
                     /translation="MEVLKVSANSQPKSVAGAIAAVVRESGAVEIQAVGAGAVNQTVK
                     SIAIARGYVSPNGIDLVCIPSFAKIDIDGEERTAMKFQLENR"
     gene            2812..>3323
                     /locus_tag="PLM30_04875"
     CDS             2812..>3323
                     /locus_tag="PLM30_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOR54536.1"
                     /translation="MSSIKAPRGVRDILPGESWKWEHVFSVTRKVAGDFGYREVHLPV
                     FEHTELFSRGVGETTDVVEKEMYTFEDRSGRSLTLRPELSASMVRSYLENRMDKQHQP
                     AKLWSAGPMFRYERPQKGRYRQFWQIDFEAIGSQDPLLDVESIELSLELFRRLGLSNL
                     EVLVNSVGCPA"
BASE COUNT          665 a          942 c         1030 g          686 t
ORIGIN      
        1 gggcgggctt gacgcgattt atccttttac tacaatgaat atccgcccgc cctgtaatgg
       61 cgaagggacc gggtcggata acgggggaag gtacctgggc atgtcgatcc aagcagcggc
      121 ggcatcggtg gtcttcatac tgacgataat ggtcatagcg acgggcaaga tgcgcaggag
      181 cgtggcggcg ctcctggcgg cgagcctgct catggggctg aacatcatcc ccgcccagaa
      241 ggcggtctcc tacatagatt tcaacaccat cgggcttctc atggggatga tgatcatcgt
      301 aggcgtcatc tccggcacgg ggcttttcca gtacatcgca gtcctcaccg tcaaggccac
      361 cggcggcagg atccacctga cctacatagc ggtgctggcg gtgacggcgc tcctctcggc
      421 tttcctcgac aacgttacca cggtcctcct cataagcccc gtggtgatat ccctcgtgga
      481 cctgatggac gtaaacccct tccccttcct cataggggag gccttcgcct cgaatatcgg
      541 cggtaccgcc accctcgtag gcgacccccc caatatgatc atcggctcct tcgcccactt
      601 ctccttcatg gatttcatcg tcaacctgac accggctgtg gccctggtga tggttatcgt
      661 gacggcctgg ctcctgtacc acttcaggaa ggacctggag ggcggcgaaa gggccacgga
      721 gcgtatcgcc aaggtcgacg agtcaaaact gataaaggac aaggggctca tgatcagggc
      781 ctcgacagcg atggtgctcg tcgtggccgg tttcgtggtc caccacatcc tggacctgcc
      841 cgcttcggtc atagcccttt tgggcgcgac ggtccttcta gccgtggtcc ccgtggacgg
      901 ggacgaggtg atccaccggg acatcgagtg gcccacgatc atgtttttcg tctcgctctt
      961 catcatcgtc ggcgccctca acgagacggg tgtcataacc gccgcttcgg aactgttcat
     1021 ttcctttttc aagggccgac cgctggccgc catggtcgcc atgctgtggt tttccggcct
     1081 ggtctgcgcc ttcatcaaca acgtggcctt cacggtcacc ttcgtatacg tagcccagga
     1141 cctggccgcc tccacgggga tggccgcggc ccccttgttc tgggccctgt cactgggggc
     1201 ctgcctggga ggcaacggaa ctttccttgg agccgcggcg aacgtggtcg tggccgatat
     1261 agcggaaaaa agcggcaacc ggatcaactt caggaccttc atgaggacgg ggctcaaggt
     1321 ggtcttcatc tccctgatca ccgctagcct ctatatcgtg atccggtggg gggggctttt
     1381 ctgatgggag acgtccctgt ttacctcccg ccccggaagg aagatacttg tcggaaccag
     1441 gaaagggggg ctcaccaatg ctcgtgggtg atctgatgga cagggacctg accgccgttt
     1501 caccgggaac gaccatcgcg gaagccgtgg agatcatggc cgggcagagg gtcacgggta
     1561 ttcccgtcgt cggtgacgac gggaaggtcg tgggcttcct cagcgagaag gatatcgtaa
     1621 aagcggcctt acccggctat ttcgaccttt tgcgggattc ctcctttttg ccggattacg
     1681 gccagttcca gaaaaggctc caggggataa gcctcgaaag ggtcgacaag tacatgcaga
     1741 cccaggtgat cgtgctggac gagcaggata cggacttgaa cgccgctatg gtgctgataa
     1801 cgaaaagcct gaagagggcc cccgtcgtaa aggacggggt cttctcgggg gtcctgagca
     1861 ggtcggacct gctggattac atacttcact ctaaaaagga accctccgac tgatggatga
     1921 aaagggcgtc cgcatcaagt tcttcggccc ctggcaacgg gccgccggga cggcggagct
     1981 ctttttgccg cttgaggagc ccgtgacggc ctccagcctt gtggagaggc tggccggcgt
     2041 ttacggggag agattccccg tccgcgagga agggctgatc tgcctatacg acggggaaag
     2101 gggccccaag gccctcgacg ggggcgagga ggtgccccca ggttccacaa tcctcttcct
     2161 gggggtcatc gaaagcggat aaggaatgga aggatcggta tgtctccttt tgagcagccg
     2221 agggcgccgt tatttcattt tcttgaaata ttcaatgggt gaggctattg ttgacagggg
     2281 aaaataaagt tatacttatc attacagttt cctaatacgc tgaagagggg atcgaaggct
     2341 acagcggaga agtatcgcgg aatccagggg gagggcgata ttccatggaa gttctcaagg
     2401 tctctgcaaa ctctcagccg aagtctgttg ccggtgcgat cgcggcggtc gtcagggaga
     2461 gcggtgccgt cgagatccag gcggtggggg cgggggccgt caaccagacg gtcaaatcca
     2521 tcgcgatagc gagggggtat gtctcgccca acgggatcga tcttgtctgc atcccttcct
     2581 tcgccaagat agatatcgat ggcgaggaaa ggacggctat gaagttccag ctcgagaacc
     2641 gctgagcctc ctcgtaaacg atccggcaag gaaaaaccgc ggcccccggg gtccgcggtt
     2701 tttttatggc ctccatcagg gaagtgaccc ggggcgggtt cgtcccggac gaaagtagtg
     2761 ctacaatcta acgatcccga tcaggcataa aaaagcaggg agggtttttt catgtccagc
     2821 atcaaagccc cgcggggggt ccgggacata ctccccggtg aatcgtggaa gtgggagcat
     2881 gttttttccg tcacgaggaa ggtggccggt gatttcggtt atcgcgaggt ccacctcccc
     2941 gtgttcgaac ataccgagct tttctcccgg ggcgtcggag agaccaccga cgtggtcgag
     3001 aaggagatgt acaccttcga ggaccgctcc ggccgaagcc ttacccttcg tccggaactc
     3061 tcggcatcca tggtgagatc ctacctggag aacaggatgg acaagcagca ccagccggcc
     3121 aagctctgga gcgcggggcc catgttccgc tatgaaaggc cccagaaggg gcgataccgc
     3181 cagttctggc agatagactt cgaggccata ggaagccagg accccctcct ggacgtggag
     3241 tccatagaac tgtccctgga gctgttcagg aggctgggcc tttccaacct cgaggtgctc
     3301 gtcaattccg tggggtgccc ggc
//
LOCUS       DAOSRG010000122         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_26102_length_3300_cov_9.168259, whole genome shotgun sequence.
ACCESSION   DAOSRG010000122 DAOSRG010000000
VERSION     DAOSRG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26102_length_3300_cov_9.168259"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLM30_04880"
     CDS             complement(<1..911)
                     /locus_tag="PLM30_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOR54537.1"
                     /translation="MKRVWSAAFAVAFLLLAAGAASSAVQPVVVGVDVTGNQEVISEH
                     ILGVIGTKAGDQFNPEQVQQDIDAIYGLGFFSFVDVNVVEQFGGVFVEFKVQENPIVR
                     DINFTGNTVFTADQLMEVVFTRPGAVFNRVFFRHDLQRIGEKYEKAGYVFVQVQDVGI
                     HDGVIDVRILEPKVGEVVIQGNKKTKAHVIEREFNLKTGDLFNANILRHSIKKINQLG
                     FLEDVRVGFEPTPDPEVVNIVLTVEEGKSSRISLTVGHGSESGWSGGAAWEELNYRGL
                     GHVVGIGFEMGDRQQYWLQYRAPYMDE"
     gene            complement(1009..>1635)
                     /locus_tag="PLM30_04885"
     CDS             complement(1009..>1635)
                     /locus_tag="PLM30_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54538.1"
                     /translation="PFSGRRLILLLEAGQEMEKNLKRVQRWIERCIAACRSERWDSAL
                     AEVECARAELESASDEIWQRASGAGEPSRAGRFVGLSFLSIAMAFLVIMVAAIPISTG
                     GYAPVGVADTRQLRLEWVTVDEQSVLSALRTSLSEMNLARETLNQDYPVEAAPAAPLP
                     VSQLSGAAPRIAREEQVAPAGTLDEILTLIEIGQRALRGEGIIIQSPE"
     assembly_gap    1636..1735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1871..2464)
                     /locus_tag="PLM30_04890"
     CDS             complement(1871..2464)
                     /locus_tag="PLM30_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR54539.1"
                     /translation="MNDKGQDHPEKEPLSREEEARLWILCGEGDEEARERLILSYRPL
                     VFWIARKYRVAPQRYQDLIQEGMLALIKAVDRFEPDRNIRFVTYGFYRIKGQMTNFLQ
                     RVEAKAPSPVDDAEVQVPDPFDADSLDWKICLADGIEALTGREGDIVRALVLEGLKAR
                     DYAESAGMDVSNVYRIQRRALAKLKAWLELGDATDRP"
     gene            complement(2525..3181)
                     /gene="cobC"
                     /locus_tag="PLM30_04895"
     CDS             complement(2525..3181)
                     /gene="cobC"
                     /locus_tag="PLM30_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HOR54540.1"
                     /translation="METRKRKILLVRHGETEWNSAFRFQGRTDVPLSERGLAQAGLLA
                     RRLARVPLDKVYTSPLLRAHQTTSAIVRLNEGVEEATAVDDLSEMSFGQWEGLRLGEI
                     RERYPDLFSAWSKDPSSTTPPGGESFPNLIVRVDRVLSEILSEEGDNLLLVCHGGTIR
                     AFLSSLMGIPPVSAWRFRTDNCSITAVDLAPDRITLRYVNDTLHTRVTPELAGSLPLL
                     "
BASE COUNT          596 a          993 c          959 g          652 t
ORIGIN      
        1 tactcgtcca tgtagggtgc ccggtactgg agccagtatt gttgccggtc acccatttca
       61 aaacctatcc cgacgacgtg gccgaggccg cggtagttga gctcctccca ggccgccccc
      121 cctgaccacc ccgactcgga accgtgtccc acggtcaggg agatcctgga agattttcct
      181 tcctcgacgg tgaggacgat gttgacgacc tcgggatccg gcgtgggctc aaatcccacc
      241 ctgacatcct cgaggaagcc gagctggttg atctttttga tggagtggcg gaggatgttg
      301 gcgttgaaga ggtcccccgt cttgaggttg aattcccttt ctatcacgtg ggccttggtc
      361 ttcttgttgc cctggatgac cacctcaccc accttgggtt ccaggatccg cacgtcaatg
      421 accccgtcat ggatgcccac atcctggacc tggacgaaaa catagccggc cttttcgtac
      481 ttctcgccaa tcctctggag gtcatgcctg aagaagaccc tgttaaagac agctccgggg
      541 cgggtgaaga cgacctccat cagctggtcg gcggtgaaga ccgtgttgcc ggtgaagttg
      601 atatcccgga cgatcgggtt ctcctggacc ttgaactcca cgaaaacgcc gccgaactgc
      661 tcgaccacat tgacgtcgac gaaggaaaag aagccgagac cgtatatcgc gtcaatgtcc
      721 tgctggacct gttcggggtt gaactggtcc ccggccttgg ttccgatgac cccaaggata
      781 tgttcggata ttacttcctg gttcccggtg acgtcgacgc cgacgaccac cggctgaacg
      841 gccgaagagg cagcgccggc cgccaaaagg agaaaagcga cggcaaaagc cgcggaccat
      901 acccgtttca atcgaacagc acccctttcc ctggactgga agcacccggc gagccggggt
      961 cacccgggta ctccccccgg atgtctcata ccttgggatt ataaactatt actcgggact
     1021 ttggataatt attccttcac ctctcagggc tctctgccct atctcgatga gggtcaggat
     1081 ctcgtcgagg gtcccggcgg gcgcaacctg ctcttcccga gcgatccggg gagcggcacc
     1141 agagagttga gacaccggca agggtgccgc gggcgccgcc tcgacggggt agtcctggtt
     1201 cagggtctcc ctggccaggt tcatttcgct caggctggtc ctcaaggccg agaggacgct
     1261 ctgctcatcg acggtgaccc actcaaggcg cagctgcctg gtgtcggcga cccccacggg
     1321 ggcataccct cccgtggata tcggtatggc cgccaccatg atgaccagga aagccatggc
     1381 gatggaaagg aaggagagcc caacgaagcg ccccgcgcgg ctgggttccc ccgcccccga
     1441 tgccctctgc catatctcgt cgctggcaga ctcgagttca gccctggcac actccacttc
     1501 cgcgagggcc gagtcccacc tttcgctccg gcacgcggcg atgcatcttt caatccagcg
     1561 ttgtacccgc ttgaggttct tctccatttc ctgaccggcc tccaacagga gtattagccg
     1621 tcgccccgaa aagggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntctcc
     1741 atttcctgac cggcctccaa caggagtatt agccgtcgcc ccgaaaaggg accttgcccc
     1801 gaaaagggac tatgccccga aaagggacct tgccccgaaa agggactatg ctccttctat
     1861 tatcccagta tcacggtcgg tccgtggcat cccccagttc gagccaggct ttgagcttcg
     1921 ccaaagcacg gcgctggatc ctgtagacgt tgctgacgtc catcccggcg ctttcggcgt
     1981 aatccctggc cttgagcccc tcgagaacaa gggccctgac gatgtcaccc tcgcgcccgg
     2041 tgagtgcctc gatgccgtcg gcgaggcaga tcttccagtc cagtgagtcc gcgtcaaagg
     2101 ggtcgggtac ctggacctcg gcgtcgtcga ccggggaggg ggcttttgcc tcgacgcgct
     2161 ggaggaagtt ggtcatctgg ccctttatcc tgtagaagcc gtaggtgacg aaacggatgt
     2221 tcctgtcggg ttcgaagcgg tcaacggcct ttatcagggc caacatgccc tcctggatga
     2281 ggtcctggta gcgttggggg gcgacgcggt atttgcgggc gatccagaag accagggggc
     2341 gataggaaag gatgagcctc tcgcgggcct cttcatcacc ctcgccgcaa aggatccaca
     2401 gccgggcctc ttcttcccgg gacaggggtt ccttttccgg atgatcctgg cctttgtcgt
     2461 tcatattgag gacctccccg gttctgctgg accctttata ccacgacagg gaggcgtttg
     2521 accactacag caacgggagg ctgccggcaa gttccggggt tacgcgggtg tgaagggtat
     2581 cattcacgta gcggagcgtg atcctgtcgg gcgccaggtc gacggctgtg atagagcagt
     2641 tgtccgtccg aaacctccag gccgacacgg gggggatacc catcaatgag gacaagaagg
     2701 cgcggatcgt ccccccgtgg cagaccaaga ggaggttgtc cccttcctcg gaaaggatct
     2761 cactcaacac ccggtcgacc cttacgatga ggttggggaa ggattcccct ccgggtgggg
     2821 tcgtggagga ggggtccttg ctccaggcgg agaagaggtc ggggtaccgt tcccgtatct
     2881 cgcctaatct gagaccttcc cactgaccga aggacatctc cgaaaggtcg tcaacggccg
     2941 tcgcttcctc cacgccctcg ttcaggcgaa cgatggcgga ggtggtctgg tgggccctca
     3001 agagggggct tgtataaacc ttgtccaggg ggaccctggc cagccgtcgg gccaggaggc
     3061 cggcctgggc cagccctctc tcactcaggg gaacatcggt cctgccctgg aagcggaagg
     3121 cgctgttcca ttctgtctct ccatgcctta caagcaagat cttccgtttt cgcgtttcca
     3181 tcgggtacct ccggtctttg tcgtttacga aaaaagccac ccgcgggggt ggcctcgatg
     3241 agccttggag ccggcgggcg gacttgaacc gccgacctgc gcattacgag tgcgctgctc
//
LOCUS       DAOSRG010000123         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_26588_length_3257_cov_11.263273, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000123 DAOSRG010000000
VERSION     DAOSRG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26588_length_3257_cov_11.263273"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLM30_04900"
     CDS             complement(<1..210)
                     /locus_tag="PLM30_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOR54541.1"
                     /translation="MSDQLFEKIPPHSIEAERAVIGGCLLEEDALTICLDALLPEDFY
                     DGSHRQAFQVMREMAEKGKPVDPLTF"
     gene            complement(203..658)
                     /gene="rplI"
                     /locus_tag="PLM30_04905"
     CDS             complement(203..658)
                     /gene="rplI"
                     /locus_tag="PLM30_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR54542.1"
                     /translation="MKVILLTDVSKVGKKGDLVDVAEGYGRNYLIPRKMAEEATRERL
                     KEWEQHQRTKEVRARREEEEAWGNRALLQGKQVMLKVSAGERGKLFGSVTATQVAERV
                     QEKFGIPVDKKDVRLPSPVKELGSYPFSIKLHQGVEAEMTLKVEDERVE"
     gene            complement(655..1614)
                     /locus_tag="PLM30_04910"
     CDS             complement(655..1614)
                     /locus_tag="PLM30_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HOR54543.1"
                     /translation="MDRTRALVESSLLVALSAVLFLAGHFLPLVGMVFSLVCPAPLVV
                     LGLRHSLGRAVLGVVAATIISAAFTGVVGALFFCFGFGFLGIALGALGRRFDRAVDII
                     LYGILVSLGSKLLLMVIAIKLTGINPFGLEEAEILPMIERISSVYSSLGMSKESLSTA
                     REQMRATLSLIPLIFPALLVMAATVDCFLSYIVSAAVLKRLGRETLPRLPEFSLWRFP
                     KSIFWALVASLALQLAGTYQPSMAFLQKTALNLRLLVTTLFFFQGLAVGWFFMGSRGL
                     GRFLKVALTLVVMVVPFFAQLVLILGIIDVWFDLRSRFRGGRE"
     gene            complement(1689..1940)
                     /gene="rpsR"
                     /locus_tag="PLM30_04915"
     CDS             complement(1689..1940)
                     /gene="rpsR"
                     /locus_tag="PLM30_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOR54544.1"
                     /translation="MAFSPRNGGGKRSKRRKPKVCMFCVEKIDHIDYKDVDKLRKYIS
                     ERGKIVPRRVTGNCAKHQRQLTEAIKRVRYLALLPFSAE"
     gene            complement(1977..2468)
                     /gene="ssb"
                     /locus_tag="PLM30_04920"
     CDS             complement(1977..2468)
                     /gene="ssb"
                     /locus_tag="PLM30_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR54545.1"
                     /translation="MARGFNKAILMGNLARDPEVRYTTSKQAVAKLTVAVGRQWKNRE
                     GEVQDQADFIPVVVWGVQAENCERYLRKGSPVLVEGRIQVRSYEAGTGERRWVTEVVA
                     QGVTFLGSRRDGGETYSPEGEQAPPSRGGSLREKGFEDDYVRDISELDDVKDEGEDAD
                     IPF"
     gene            complement(2481..2765)
                     /gene="rpsF"
                     /locus_tag="PLM30_04925"
     CDS             complement(2481..2765)
                     /gene="rpsF"
                     /locus_tag="PLM30_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR54546.1"
                     /translation="MRSYEMVTIFQADLEEHESAVGEIEGVVRNLGGEIVKSSSWGKR
                     RFAYPIQKQTEGFYAVLTFTLDPSQVSELERLLKLKTQVLRHLVIALDET"
     gene            complement(2842..3147)
                     /locus_tag="PLM30_04930"
     CDS             complement(2842..3147)
                     /locus_tag="PLM30_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HOR54547.1"
                     /translation="MIRLRWVALITAGLCFLAIVGTAYILELRKIGRLGSIVDERMDR
                     LVAVTRDVQVLKEKILFYRTPEGVARLAREQFNLTLPGERIFRVEVVSGDLLPEEGP"
     gene            complement(3150..3257)
                     /locus_tag="PLM30_04935"
     CDS             complement(3150..3257)
                     /locus_tag="PLM30_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOR54548.1"
                     /translation="MGPELEGARLVLSEATRVVLANALSLLGISAPEKM"
BASE COUNT          680 a         1075 c          834 g          668 t
ORIGIN      
        1 aaaggtgagg gggtccacgg gtttcccctt ttcggccatt tccctcatga cctggaaggc
       61 ctgccgatgg ctcccgtcgt agaaatcctc cggcaggagc gcgtccaggc atatggtcaa
      121 ggcatcctcc tcgagaaggc agccccctat caccgccctc tcggcttcga tgctgtgggg
      181 ggggatcttc tcgaagagct ggtcactcaa ccctttcgtc ctccaccttc agggtcatct
      241 ccgcttcaac cccctggtgc agttttatgg aaaaggggta ggaacccaac tccttcaccg
      301 gtgaaggaag ccggacgtcc tttttatcga cggggatgcc gaacttttcc tggacccttt
      361 ccgccacctg ggtcgccgtg acgctcccga acagcttccc cctctccccg gcggaaacct
      421 ttagcatgac ctgcttcccc tggaggaggg ccctgttgcc ccaggcttcc tcctcctccc
      481 gcctggccct gacttccttc gttctctggt gttgttccca ctccttcaac ctctccctgg
      541 tcgcctcttc cgccatcttt ctcgggatca ggtaattcct gccatacccc tcggccacgt
      601 cgaccaggtc gccctttttc cccacttttg acacatccgt cagcagtata actttcattc
      661 tcttcccccc cggaatctcg accgcaggtc gaaccacaca tcaatgatac ccaaaataag
      721 gaccaactgc gcaaaaaagg ggacgaccat gacgaccaat gtcaaggcaa ccttcaggaa
      781 acggcccagc cccctggaac ccatgaaaaa ccagcccaca gccagcccct ggaaaaagaa
      841 cagggtcgtt accaggaggc gaaggttcag ggcagtcttt tgcaaaaagg ccatcgaggg
      901 ttgataggtc cccgccagtt ggagcgccaa ggaggccaca agggcccaaa aaatgctctt
      961 cgggaagcgc cacagactga actccgggag ccggggaagg gtttccctgc ccagtctttt
     1021 cagcactgcg gcgctcacta tgtagctcag aaagcagtcc accgtcgctg ccataacaag
     1081 aagagcggga aagataagcg ggatcaggga gagggtggca cgcatctgtt ccctggccgt
     1141 ggacaatgac tccttcgaca tgcccaggct tgagtagacc gaggagatcc tctcgatcat
     1201 gggcaggatc tccgcttcct cgagcccgaa gggattgatc cccgtgagct ttatggcgat
     1261 caccatcagg aggagtttgc tccccaggga aaccaggatc ccgtaaagga tgatatcgac
     1321 ggccctgtcg aaacgcctgc ccagggcccc gagggctatg cccaggaaac cgaaaccgaa
     1381 gcagaagaaa agggcaccca ccacacctgt gaaggcggcc gagatgatcg tcgccgccac
     1441 tacccccagc accgcccttc ccaggctgtg tctaagaccc aggactacga ggggcgctgg
     1501 gcaaaccagg gagaacacca tcccaaccag gggcaggaaa tgacccgcca gaaaaagcac
     1561 cgcggaaagg gcgacgagca acgatgattc aaccagggcc ctggtcctgt ccataaaaaa
     1621 cgacctcccg acagaacggg gggagaagcc tgggctcctc cccccgcaac gaacacgggt
     1681 atcctctcct actcggcgct gaaggggagg agagcgaggt agcgcaccct tttgatcgcc
     1741 tccgtgagct gcctctggtg cttggcacag tttccagtca ccctcctggg gacgatcttg
     1801 ccgcgctcgc ttatgtactt tctgagttta tccacatcct tgtaatcaat gtggtcaatt
     1861 ttctccacgc agaacatgca gaccttcggt tttcttctct tgcttctctt gcctccgcca
     1921 tttcttggtg aaaaggccat cacgaacctc cttcttttca agggatccaa aaaacatcag
     1981 aagggaatat ccgcgtcctc gccctcgtcc ttgacgtcgt ccagctcgga tatatccctc
     2041 acgtaatcgt cctcaaaacc cttttctctg aggctgccgc ccctcgacgg gggcgcctgt
     2101 tcgccttcgg gggagtaggt ctccccgccg tccctgcggg aaccaagaaa cgtcacccct
     2161 tgggcgacta cttcggtgac ccaccgtctt tcgccggttc ccgcctcata acttcggacc
     2221 tggatccttc cttcgacgag gacagggctc cccttacgga ggtacctttc acagttctcg
     2281 gcctggacac cccataccac aacggggatg aagtcagcct gatcctggac ctcgccttcg
     2341 cggttcttcc actggcggcc tacagccacg gtcaatttcg ccacagcttg cttggaagtg
     2401 gtgtagcgca cctcgggatc ccgcgccagg ttgcccatca gaatagcctt gttgaaaccc
     2461 cttgccatcg agatcactcc ctaggtttca tccagagcaa tcaccaggtg tcttaaaacc
     2521 tgggtcttga gcttcagcaa ccgttccagt tcgctgacct gggaaggatc gagagtaaag
     2581 gtaaggaccg cgtagaaccc ctcggtctgt ttctggatgg gataggcgaa gcgcctcttc
     2641 ccccaggaac tgctctttac gatctctccc cccaggttgc gcacgacccc ttcgatctct
     2701 cccaccgccg actcatgttc ctccaggtca gcctggaaga tcgtgaccat ttcgtacgat
     2761 cgcacgtcat acacctcctc cctttggcct gaggtctccc caccccgggg gagacaggga
     2821 catacggcag aacattatac gctaggggcc ctcttccggc aacaggtccc ccgaaaccac
     2881 ctcgacccgg aagatccgct ctccggggag ggtcaggttg aactgctccc gtgcaagacg
     2941 ggccacgcct tcgggtgtcc tgtaaaaaag tattttttcc ttcaggacct ggacatccct
     3001 cgtaacagcg accaggcggt ccatcctttc gtccacgatg gagcccagcc tgccaatctt
     3061 cctgagttcc aggatgtaag ccgttccgac gatcgccagg aagcagagac cggccgtgat
     3121 cagggccacc cagcgcaggc ggatcattcc tacatcttct ccggtgcgct tatccccagc
     3181 agggaaaggg cgttggccag gactaccctc gtcgcttcgg agaggacgag cctggcgccc
     3241 tccagttccg gtcccac
//
LOCUS       DAOSRG010000124         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_26663_length_3250_cov_9.983412, whole genome shotgun sequence.
ACCESSION   DAOSRG010000124 DAOSRG010000000
VERSION     DAOSRG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26663_length_3250_cov_9.983412"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="ispG"
                     /locus_tag="PLM30_04940"
     CDS             complement(<1..941)
                     /gene="ispG"
                     /locus_tag="PLM30_04940"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOR54549.1"
                     /translation="MPGRPVMVGSLGVGSGFPVRVESMLKTPLQDLPGCLEETRSLAE
                     EGCELVRVAFPAMGLSGELSRLIEGSPIEVMADIHFDHRLAIGAIDSGCRSVRINPGN
                     MGGHRGMRELSRAVKGNGVVVRIGANSGSLSRSQMEKAGGDTGRALFDAVSEQVGLLL
                     DWGVEEMILSAKSSSIKTTLRANSMIASAFDAPIHIGLTESGPDQRGVIKSSVAVGSL
                     LGSGIGDTIRISLTGTSREEVIVGKRILQSSGVRRFEAELVSCPGCGRRRVEVDSLVA
                     MIEPFLVELRKDLRVAVMGCEVNGPGEAADSDIGIAGT"
     gene            complement(966..2012)
                     /locus_tag="PLM30_04945"
     CDS             complement(966..2012)
                     /locus_tag="PLM30_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOR54550.1"
                     /translation="MMGLSALAFVLVIAICVISHEFGHLVVARSAGVQVHEFSFGMGP
                     LLVGWKRGGMIWSIRAVPVGGFVRLAGMSEEQGEEEVLPGMSFAEKSPWKRLAILGGG
                     SVSNIILAILFTAFLLWGHGIMDLESTRIGEVMAGYPAEELGLRPGDVVKAVSGVTVG
                     DWATLSSTIREKAALGPVGLTFERDGVTYVLQGKVPMSPEYKVPLLGIRPSMKRYPFI
                     KALSGSLGYIGDFSIDIVKGIWSWATGNGQVEITGPLGIASMAGKAAREGFWTFLAFL
                     SMISLHLGILNLLPFPALDGGRILLVMGEIMTGKKLPEKWESLVHLAGFILLIILLIF
                     VSWKDLIKLLPVAR"
     gene            complement(2043..3185)
                     /gene="dxr"
                     /locus_tag="PLM30_04950"
     CDS             complement(2043..3185)
                     /gene="dxr"
                     /locus_tag="PLM30_04950"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOR54551.1"
                     /translation="MEARNIAVIGATGSVGRSVLEVCSFFPDLFRVRALAAEKNAEGL
                     LGLGRRFNSDLLVLADQASSEKLRRMAGSEFTCRGGEEALLSLVEEPLCEEVAFASSG
                     TGALPALIGALQKGKKVYLANKEILVAAGEWVMPLAQGNIIPLDSEHNAVWQCLRGEA
                     EQSVERVYLTASGGPFLHSTLEELREVTFERASRHPVWPMGKKISVDSATLMNKGIEM
                     IEAHHLFGLPIGKIEAVIHPQALIHGMVEFIDGSIKMLYSKPDMKLAIMSAFGMEGRL
                     ANPDPDLSPPGFPDMDLVFMEPDEERFPCLALAKEACRLGGAYPPLLVGADEGTVNLF
                     MEGRISFTDIAPTIERVLSSCPRERPRSWKDALGLVTWARERVLKG"
BASE COUNT          691 a         1063 c          856 g          640 t
ORIGIN      
        1 gtaccggcaa tgccgatgtc ggaatccgcc gcttccccgg ggccgttcac ttcgcaaccc
       61 atgaccgcga ccctcagatc cttcctgagt tcgaccagga agggttctat catggccacc
      121 agggaatcca cttcgaccct tctcctgccg cagcccgggc aggagaccag ttccgcctcg
      181 aatcgcctga cgcccgaaga ctgaaggatc cttttgccga cgatcacttc ctcccgggaa
      241 gtaccggtga ggctgatcct tatcgtatcg ccgatcccgc tcccgagcag cgacccgacg
      301 gcgacagagc tcttgatcac acccctttgg tccggccccg actcggtcag ccctatatgg
      361 ataggagcat cgaaggctga cgcgatcatc gaattggccc tcagggtcgt ctttattgat
      421 gaggatttcg cggaaaggat catttcctcg acgccccagt ccagcaggag ccctacctgt
      481 tcgctgacgg catcgaacaa ggccctgccg gtatccccgc cggctttttc catttgggac
      541 ctcgagagcg agcccgagtt agcccctatc ctcaccacta ctccattgcc cttgacggcg
      601 cggctcaact ccctcatccc tcgatgaccg cccatgttac ccgggttgat cctgacggac
      661 cggcaaccgg agtcgatcgc cccgatggca agtcgatggt caaaatggat gtcggccatt
      721 acctctatcg gtgagccttc tatcaaccgc gacagctccc cggaaagtcc catcgctgga
      781 aaggccaccc tgacgagttc gcaaccctct tctgccaggg accgggtttc ctcaaggcac
      841 ccgggaaggt cctgaagcgg ggtcttaagc atgctttcca ccctgacggg gaaaccactc
      901 cccaccccaa gggatcccac catcacaggt cttccgggca tttccctttt cgacctccct
      961 tcggcctacc gggcaactgg aaggagtttg atcagatcct tccaggagac gaagatgagc
     1021 aaaatgatca aaaggataaa acccgcaagg tggaccaggc tctcccattt ttcggggagc
     1081 ttcttgcctg tcatgatctc ccccatgacg aggaggatcc tccccccgtc gagggccggg
     1141 aaggggagta ggttcaggat gcccaggtgg agactgatca tggacaggaa ggctagaaag
     1201 gtccagaaac cctccctggc cgcttttccc gccattgacg ctatccccag gggaccggtg
     1261 atctcgacct ggccgttccc cgtcgcccat gaccatatcc ccttgacaat atcgatgctg
     1321 aaatccccta tgtaacccag ggagcctgaa agggccttaa tgaaggggta cctcttcatg
     1381 gaaggtctta tccccagaag cggaaccttg tattccggag acataggcac cttgccctgg
     1441 agaacatagg tcacgccgtc ccgttcaaag gtcaatccca ccggccccag agcggctttt
     1501 tcccttatgg tggatgaaag ggtggcccag tctcccacgg tcaccccgga gactgctttt
     1561 acaacatccc ccggccgtag gccaagctcc tccgccgggt atcccgccat gacttccccg
     1621 atacgggtgc tctccaggtc cattatcccg tgcccccaaa ggagaaaggc cgtgaagagg
     1681 atcgccagga tgatgttcga aaccgagccg ccgccgagga tcgcaaggcg tttccagggt
     1741 gacttctcgg cgaaggacat gccgggcagt acctcttcct ctccctgctc ttccgacatt
     1801 ccagccagcc ggacgaatcc cccgaccggc accgccctaa tggaccaaat catgccgcct
     1861 cttttccacc cgacgagaag aggccccatc ccgaaggaaa actcgtgaac ctggacaccc
     1921 gcggaccttg cgaccacgag gtgcccgaac tcatgcgaaa taacgcagat cgctatcacc
     1981 agtacaaaag ccaacgcact caagcccatc atttcatccc tttcgggcag caaataaccc
     2041 gcctaaccct ttaaaaccct ttccctcgcc caggtcacga gtccaagggc atccttccag
     2101 gaacggggtc tctccctggg acaggaggat aacactctct cgatcgtcgg ggcgatgtcc
     2161 gtaaaagaga tccgcccctc catgaacagg ttcacggtcc cctcgtcggc cccgactagg
     2221 agcgggggat aggccccgcc aaggcggcag gcttccttgg ccagagcgag acaggggaat
     2281 ctctcttcat cgggctccat gaacaccagg tccatgtcgg gaaaccccgg cggggacagg
     2341 tccgggtccg ggtttgccag gcgaccttcc atcccaaagg cggacattat cgccagcttc
     2401 atatccggct tggagtagag cattttgatc gatccatcga taaattccac catgccgtgg
     2461 atcagggcct ggggatggat caccgcttct atcttcccga tgggcagccc aaaaaggtga
     2521 tgggcctcga tcatctcgat acccttgttc atgagggtgg cgctatcgac ggagatcttt
     2581 ttccccatgg gccagaccgg gtgacggctg gccctttcaa aggtgacctc ccgcagctcc
     2641 tccaaagtgc tatgaaggaa aggcccaccg gacgcggtga gatagaccct ttccacggat
     2701 tgttccgcct cgcctctaag gcactgccag acggcgttat gctcgctatc caggggtatt
     2761 atattcccct gggccagtgg catgacccat tcgcctgcag ccacgaggat ctccttgttg
     2821 gcgaggtaga cctttttccc cttttggagc gcccctataa gggcgggaag ggctcctgtc
     2881 ccggaagagg cgaaggccac ttcttcgcag aggggctctt cgaccaatga aaggagagct
     2941 tcctccccgc ctcggcaggt gaactccgat cctgccatcc tgcggagttt ttctgaagag
     3001 gcttggtcgg ccaggaccaa aaggtcacta ttgaatcgcc ttcccagtcc taacaacccc
     3061 tcagcgttct tctccgcggc gagagcccgg acccggaaaa ggtcggggaa gaaggaacag
     3121 acttcaagga cagatcgccc cacgctcccc gtggcaccga tgacggcgat gttcctggct
     3181 tccatggtta ccagagcacc ccgaagacga aaaacaccag gaccgagtta acgaggatgc
     3241 tgtcgaacct
//
LOCUS       DAOSRG010000125         3235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_26817_length_3235_cov_6.550943, whole genome shotgun sequence.
ACCESSION   DAOSRG010000125 DAOSRG010000000
VERSION     DAOSRG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26817_length_3235_cov_6.550943"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLM30_04955"
     CDS             complement(<1..1168)
                     /locus_tag="PLM30_04955"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOR54552.1"
                     /translation="MKVLVLNCGSSSLKYQLFDMTTEAVLAKGLVERIGIEGSRVRHE
                     KTGLEPLKFDTPIPDHKVGIKVVLDTLLDGDQGVIKNLEELRAVGHRVVHGGEKFARS
                     VVVNPEVLKVVEECCPLAPLHNPANLMGIKAMMEVLPNVPQVAVFDTAFHQTMPDYAY
                     TYGLPYHYYEKHRVRRYGFHGTSHFYVAHRAAEMMGRPIEDLKVVTCHLGNGSSISAV
                     KRGKSIDTSLGFGTVPGVLMGTRCGDIDPTVILYIMEHEEIDPKSMSHIIHKESGFKG
                     ITGISSDLRDVEEAAVQGNGKARLALDILFYGIRKHICAYAGAMGGIDAIVFTAGIGE
                     NSITVRKMVCEGLEFLGAKIDLEKNKVRSKEAFINVPDSRVSILVIPTNEELVIA"
     gene            1256..2470
                     /locus_tag="PLM30_04960"
     CDS             1256..2470
                     /locus_tag="PLM30_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOR54553.1"
                     /translation="MFKKNIVGIIAEYNPIHNGHLYHINRSLETSGADAVVVVLSSSF
                     VQRGEPAFLDKWSRAEMALHSGADLVIELPTAFSCHNAGVFGSAAVDLMAMTGIVTHL
                     SFGMEDPDAPLEAIARILVEEPSPFRKILRERLDSGFSYAEARTAAMEQMLPGAGAIL
                     SSPNNILAQEYVLRIVERGYPLETTPVKRTGTGYHSGPSGSFAGAGWIRGAVRAEGIT
                     DPVAKVMPPHALSSLREEISRGRCVLSLDLFWRILRAVLLREGPSGISKYAEMREGIE
                     NLLWANAVKARSFEEFTSLCTSRRYPGSRIRRSACHSLIGYEHQANRKAQRLGPPYIR
                     VLGWDAKGQEVLRMMKTSSKLPVLFRPKGRPGTYQRMIADLEYRATALWESLVPNPEP
                     LSESKKGPLRLV"
     gene            complement(2457..2945)
                     /gene="coaD"
                     /locus_tag="PLM30_04965"
     CDS             complement(2457..2945)
                     /gene="coaD"
                     /locus_tag="PLM30_04965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR54554.1"
                     /translation="MYPGSFDPITNGHIYIAERAAAIFGDLTVGVLNNSQKKYTFSKE
                     ERETMTREALSHLPNIRIKHFEGLLVDFMRAEQCRVIIRGLRALSDFEYEFQLALMNR
                     QLAPEIETFFIVTEAKYSYLSSRAVKEVFHFGGAIHDMVPPGVFRRLRERFPPVPGFR
                     QA"
BASE COUNT          710 a          938 c          868 g          719 t
ORIGIN      
        1 tcgcaatgac gagttcctcg ttggtgggta tgaccaggat gctcaccctg gagtcaggaa
       61 cgttaataaa ggcctcttta cttctaacct tgttcttttc gaggtcgatc ttcgccccaa
      121 ggaattcgag gccttcacag accattttcc ttacggtgat gctgttctct ccaatgccgg
      181 cggtgaatac gatggcgtca atgcctccca tggccccggc ataggcacaa atatgctttc
      241 gaataccgta aaaaaggatg tccagggcga gtcgggcttt gccatttccc tggacggcgg
      301 cctcctccac atccctgaga tcactgctga tgcccgtaat gcctttgaaa ccgctctcct
      361 tgtggataat atggctcatc gacttgggat cgatctcttc atgttccatg atataaagga
      421 taacggtggg atcgatgtcc ccgcaccggg tccccatcag gacaccaggg acggtcccga
      481 acccaaggct ggtgtcaatg gacttgcctc tcttgacagc cgaaatggag ctaccattgc
      541 caagatggca tgttactacc ttcagatcct cgatcggccg gcccatcatc tcggcagccc
      601 tatgggcaac gtaaaagtga ctggtaccat ggaacccgta acgcctgacc ctgtgctttt
      661 catagtaatg gtagggaagg ccgtaggtgt aggcataatc cggcatagtc tggtggaagg
      721 cggtatcgaa aaccgcgact tggggaacgt tcggcaggac ctccatcata gcctttatcc
      781 ccatgaggtt ggccgggtta tgtagcggcg ccagggggca gcattcctcg acaaccttca
      841 gtacctcggg attcaccact acggagcggg cgaatttttc accaccgtgg accaccctgt
      901 ggcctacggc cctgagttcc tcgaggttct tgatcactcc ctggtcccca tcaagaaggg
      961 tatcgagaac aaccttgatc cccaccttat ggtcggggat aggggtatcg aacttcaggg
     1021 gctcgagacc tgttttttcg tgccgaaccc ttgaaccctc gatgccgatc ctctccacca
     1081 gcccctttgc cagaacggct tccgtcgtca tatcgaaaag ctggtacttc aaagaagagc
     1141 tgccgcagtt gagaaccagt actttcatgc tcgtccagac ctccttcggt attgaccgga
     1201 accgttccac agatctatcg tattcttgat cgaccaggaa ggaaggtccg ggcccttgtt
     1261 caaaaagaat atagtaggga tcattgccga gtataacccc atccacaatg gacatttata
     1321 ccatatcaac cggtctctgg aaacaagcgg cgctgacgcc gtggtcgtgg tgctttcatc
     1381 gagctttgtc cagagaggcg aaccggcgtt cctggacaag tggagccgtg ccgaaatggc
     1441 cctccattcc ggtgccgatc ttgtaataga actcccaacg gccttctcct gtcacaacgc
     1501 aggggtcttc ggctcggccg cggttgacct gatggccatg accggtatcg tgacccacct
     1561 ctcctttgga atggaagacc ccgatgcccc ccttgaggcg atagcgagga tcctcgtcga
     1621 agaaccctcc cccttcagga agatcctaag ggaacgtctt gacagcggat tttcttacgc
     1681 cgaggccagg accgcagcca tggaacagat gctgccgggg gcgggcgcga ttctttcatc
     1741 ccccaataat atcctggccc aggagtacgt cctgaggatc gtcgaacggg gttaccccct
     1801 ggaaaccact cccgtcaaaa ggaccgggac tggttaccac tcaggcccct caggatcctt
     1861 cgccggcgca gggtggatca ggggtgccgt aagagccgaa ggtataaccg accccgtggc
     1921 gaaggtgatg ccccctcacg ccctttcttc gctaagggaa gagatctccc ggggcaggtg
     1981 tgtactctct cttgacctat tctggaggat cctgcgggcc gtcctgttga gggagggccc
     2041 ctcagggatc tcgaagtacg ccgagatgag ggaggggatc gagaatcttc tttgggccaa
     2101 cgccgtgaag gcccgatcct tcgaagaatt cacaagcctt tgtaccagcc gccgatatcc
     2161 cgggagcagg atacggcgat cggcctgtca ctccctgatc gggtatgaac accaggccaa
     2221 caggaaggcc caaaggctgg gtccccctta catcagggtc ctcggttggg atgcgaaagg
     2281 gcaggaggtc ctgaggatga tgaaaacctc ttcgaaactc cccgttctat tccgccccaa
     2341 agggagaccc gggacatacc agcgaatgat cgccgatctc gaatataggg ccacggccct
     2401 ttgggaaagc ctcgtcccga accctgaacc gttgagcgaa tcgaagaagg gccctctcag
     2461 gcttgtctaa agccgggcac gggcgggaat ctctccctca gcctccggaa aacccccggt
     2521 gggaccatat catggatggc tcccccgaaa tggaagactt ccttcaccgc ccggctcgaa
     2581 aggtaggaat acttggcttc ggtgacgatg aaaaaggtct ctatctccgg tgccagctgc
     2641 cggttcatca gggccaactg gaactcatat tcgaaatccg aaagggctct caacccccgg
     2701 atgatgacac ggcattgttc ggccctcatg aaatcgacca ggagaccttc gaaatgcttg
     2761 atccgtatat tgggaaggtg gctcagggct tcccttgtca tggtctccct ttcttccttg
     2821 gagaaggtgt acttcttctg gctgttgttg agcacaccca cggtgaggtc gccgaatata
     2881 gcggcggccc tttcggctat gtaaatgtgc ccgtttgtaa tggggtcaaa ggatccgggg
     2941 tacatggctc ttactaatat ggtggttccc tcccttcaat tgttttttgg aaaaaagtca
     3001 acaacgtgtc tccgtaggcc ctcgtccgga aagaccaccc atcccattgg gccgggacga
     3061 ccacttccct cttcgaatgc tccatgacga agataccttt atccttcatg acagcctcca
     3121 ggcgaaacct cgtggaagct atcctagtga cccaccccat cccatagggg ggatcagcga
     3181 acaccacgtc aaaaaggcgg ccctggtttt ccaggaggga gagggccttt cggac
//
LOCUS       DAOSRG010000126         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_27492_length_3177_cov_8.036515, whole genome shotgun sequence.
ACCESSION   DAOSRG010000126 DAOSRG010000000
VERSION     DAOSRG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27492_length_3177_cov_8.036515"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1207
                     /locus_tag="PLM30_04970"
     CDS             <1..>1207
                     /locus_tag="PLM30_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR54555.1"
                     /translation="RMKKDGSRFPVAIWGVPIIQGDRVLAVYAIYEDISERKAAEREL
                     ARERSLLERIVVDSPDGTVLFDEDGKIFRFNPAFDRLFDLGQDEVLGHHLWEILGGGG
                     KEEEIRGNIRQLRDRKKLDYDAVRFRKDGSEIHLSIRAVAITGDGPSGEYLAVYRDIS
                     SRVMAQHQLATERTYFENLFMNSPLAIALVGGDGIIQRVNDSFERLFGYYSFECVGAD
                     LDALIAPGDLLGDAVGLTRGAAAGSTIKAERTRRRKDGSWVEVQINAVCFPVGDGPSV
                     IYAIYQDITERKVLDEKIRYFGCHDALTGLYNQAFFEEELRRLDSPRQLPLSLVVADL
                     DNLKLINDAFGHLEGDKLLAEAGTILRSCCRQEDIIARCGGDEFVMLLPQTTLLEARS
                     VCDRVRRAC"
     assembly_gap    1208..1307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1447..2391)
                     /locus_tag="PLM30_04975"
     CDS             complement(1447..2391)
                     /locus_tag="PLM30_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54556.1"
                     /translation="MSGLLLSTKGLSVTFHSPRGSAEALKGADIDVAPGESLAVVGES
                     GSGKTTLLRASLGLVPASAGSVTLLGRKLAGCDSKDLISLRRRCGFIPQDPFECVPPT
                     LNALDAASEPIRVAGRGSRGEALEKAGVLLAELGLGDPALWKERVRLSLSGGQRQRVS
                     IARALSLDPEFLLADEPASMQDAANRAEVMAILRRRTRQGMGLLMATHDLPLAAAFAD
                     RVAVMYKGAVVETGPSESVAGNPLHPYSRALIAAIPGIGKEIRPPERVGEWPFGGCPY
                     APRCPARTERCREVPPLSPVAPDRNVACWNAREGGLLK"
     gene            complement(2388..>3177)
                     /locus_tag="PLM30_04980"
     CDS             complement(2388..>3177)
                     /locus_tag="PLM30_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54557.1"
                     /translation="GAMIAIRDLSVTYRTAARSVPAVRGVSLDVPRGRVTAVVGESGG
                     GKSSLVYGILRLLPPGTEMSGSIGFGGMELTRTSEEKMREFRWKKAALVTQGAMNSLT
                     PVLTVGYQIAEVLRVRLGMAGDEARKRVAELLSMTSLPPGYARKYPHELSGGEKQRAV
                     ISMAVACSPEFLIADEPTSALDVITQAEVIATLVRMVRDKGMGLLLVTHDLPLALSIS
                     DEMAVMHEGRLVEKGTPERITTSPSHAHTRTLLSAFSLTARSGP"
BASE COUNT          523 a         1025 c         1011 g          518 t
ORIGIN      
        1 tccggatgaa gaaggacggt tcacggtttc ctgtcgcgat atggggcgtc cccatcattc
       61 aaggcgacag ggtgctggct gtctacgcca tctacgagga tataagcgag cggaaggccg
      121 cagaaaggga actggctcgc gaaaggtccc tgctggaaag gatagtggtc gattcgcccg
      181 acggcacagt cctcttcgat gaggatggga aaatctttcg tttcaacccg gccttcgacc
      241 ggctcttcga cctggggcag gatgaggtcc tcggacatca cctctgggag atcctggggg
      301 gcgggggaaa ggaagaagag atccggggta atatccgtca actgagggac cggaaaaagc
      361 tggattatga cgccgtccgg ttccgcaagg acggcagcga gatacacctc tccataaggg
      421 cagtggccat aacgggcgac ggcccctccg gggaatacct ggccgtctac cgggatattt
      481 catcgagggt gatggcacag caccaactcg ccacggaaag aacctacttc gagaacctct
      541 tcatgaacag ccccctggcc atagccctcg tcgggggcga cggcatcatc cagcgggtca
      601 acgattcttt cgagaggctc ttcgggtact acagttttga gtgcgtcggc gcggacctgg
      661 acgcgctcat agcccccggc gatctcctgg gcgatgccgt gggcctgacc aggggggcgg
      721 cggccggttc caccatcaag gccgaaagga cgaggcgccg gaaggacggg agctgggtgg
      781 aggtgcagat caacgccgtc tgcttccccg ttggggacgg gccgtcggtg atctacgcca
      841 tataccagga cataaccgag aggaaggttc tcgacgaaaa gatcaggtac ttcggctgtc
      901 atgacgccct tacgggcctg tacaaccagg ccttcttcga ggaggaactc cggcggctgg
      961 actcgcccag gcagctcccc ctcagcctgg tcgtggcgga cttggacaac ctgaagctca
     1021 tcaacgacgc cttcggccac ctcgaaggcg acaaactcct ggccgaggcg gggacgatcc
     1081 tccggtcctg ctgcaggcag gaggatatca tcgcccgttg cggcggcgac gaattcgtca
     1141 tgctcctgcc ccagacgacc ctcctcgagg ccaggtccgt ctgcgaccgc gtaaggcggg
     1201 cctgcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac cggcccagcc
     1321 tttccagcga ggaggccctc caggagatag cctcccaggc gggcaaacag ttcgaccccc
     1381 gactggcggg ggtcttcgtc cagctccaca ggaccctgga ggtcggcaac ggccttccgt
     1441 aacccttcac ttcaatagcc ccccttcacg ggcgttccag caggcgacgt tccgatccgg
     1501 tgccaccggc gagagagggg ggacctccct gcagcgctcc gtcctggcgg ggcaccgggg
     1561 agcgtaagga caccccccga agggccattc cccgaccctt tcggggggtc tgatctcctt
     1621 ccctatcccc gggatggccg cgatgagggc cctgctgtag gggtgaaggg ggttccccgc
     1681 gacgctttcg gagggccccg tctcgacgac ggcccccttg tacatgacgg ccaccctgtc
     1741 ggcgaaggcc gccgccaggg ggaggtcgtg agtggccatc aggagcccca tgccctgtcg
     1801 agtcctgcgg cggaggatgg ccatcacctc agccctgttg gcggcgtcct gcatgctcgc
     1861 cggttcgtcg gccaggagga actcggggtc caggctgagg gccctggcga tgctgaccct
     1921 ctgcctctgt ccccccgaga gggaaagcct cacccgctcc ttccagaggg ccgggtcgcc
     1981 caggcccaac tcggccagga ggactccggc cttttccagg gcctccccgc ggctccccct
     2041 gccggcgacg cggatcggtt cggaagcggc gtccagggcg ttcagcgtgg gggggacgca
     2101 ctcgaaggga tcctggggga tgaagccgca tctcctcctc aatgagatca gatctttact
     2161 gtcgcacccc gccagtttcc gccccaggag ggtgacggag cccgcggaag ccgggaccag
     2221 ccccaggctc gcccggagca gcgtggtctt cccgctcccc gattcaccga cgacggccag
     2281 gctctcgccg ggcgcgacat cgatgtccgc tcccttcagg gcctccgcgc tgccccgggg
     2341 tgaatggaac gtgaccgaca ggcccttcgt ggagaggaga aggccgctca cggtcctgac
     2401 ctcgccgtga ggctgaaggc tgaaagcagc gtcctcgtgt gggcgtggga gggggaggtc
     2461 gtgatccgct caggggtccc cttttcgacc aggcggccct cgtgcatcac cgccatttcg
     2521 tcgctgatgg agagcgccag cgggaggtcg tgggtcacga gaaggaggcc catcccctta
     2581 tccctcacca ttctgacgag ggtggcgatg acctcggcct gggtgatcac gtcgagggcg
     2641 ctcgtcggct cgtcggcgat caggaactcc ggggaacagg cgacggccat cgatatgacc
     2701 gcccgctgct tctcgccgcc ggagagttcg tgggggtact tcctcgcgta gcccggcggc
     2761 aggctcgtca tggacagcag ttcggcgacc ctcttccggg cctcgtcgcc ggccatgccc
     2821 agcctgaccc ggagcacctc ggcgatctgg tagcccacgg tcagcaccgg cgtcagggag
     2881 ttcatggccc cctgggtcac cagggccgcc ttcttccagc ggaactccct catcttctcc
     2941 tcgctcgtcc gggtcagctc catgccgccg aagcctatgc tccccgacat ttccgtgccg
     3001 ggggggagga ggcggaggat gccgtagacc aggctgctct tgccgccccc cgattccccg
     3061 acgacggcgg tcactctccc ccggggcacg tccagggaga cgcccctgac agcggggacg
     3121 ctcctggcgg cggtcctata ggtcaccgac aggtcccgga tcgcgatcat cgccccc
//
LOCUS       DAOSRG010000127         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_27547_length_3171_cov_10.786585, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000127 DAOSRG010000000
VERSION     DAOSRG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27547_length_3171_cov_10.786585"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLM30_04985"
     CDS             complement(<1..884)
                     /locus_tag="PLM30_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR54558.1"
                     /translation="MPRKDITPRNPEEDAEEEREKGTGMVERGLREILDSVPELILRF
                     TPDLVTTFANRTYCEYHGVRYEDVVGKSFKDHVHPGDMPSLKEKLRGLTPESPVLAGV
                     ERTLMPDGEIRWQEWTDRGIFDPRGNLVEIQSIGRDVTERKRTEEAMAFERESLDALF
                     GYSMEGIVFCDHDLTVLKANPSFCDMFGYAPEEVLGRNIDDLVAGGTEYLEEARGFSA
                     ATAGGRAVAAEATRRGKDGSLIQVSCLGIPVCLSDGRRFAYGIYRDITARKAAERALK
                     ESEAKYRELFDSMPVGFYT"
     gene            1099..2367
                     /gene="eno"
                     /locus_tag="PLM30_04990"
     CDS             1099..2367
                     /gene="eno"
                     /locus_tag="PLM30_04990"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOR54559.1"
                     /translation="MAAVRAMEILDSRGNPTVRTWCRLDGGIEVTASVPSGASTGENE
                     AVELRDGDMARFGGKGVLRAVENVNAIIAPRLVGMDPTHQEEIDRLMIELDGTANKGA
                     LGANAILGVSMAVAKAASRARDLPLYAYLGGPGAARLPVPMMNILNGGKHADNSVDLQ
                     EFMVMPVGAPTFAEALRYGTETFHALKKILSSRGYVTAVGDEGGFAPDLGSNDEACRV
                     IVEAIEAAGYTPGRDIALALDPAASSFYEDGAYHLARSGQGRRTSDQMTALYGEWVEK
                     YHVVSIEDGLDENDWEGFRAQTAELGDRVQIVGDDLFVTNARFVERGIREGSANAVLI
                     KLNQIGTVTETVETIKMCREAGWGYVISHRSGETEDTFIADLAVAMGGGQIKTGSACR
                     SERVAKYNRLLEIEDQLGGRAVFRNPFEGR"
     gene            complement(2377..>3171)
                     /locus_tag="PLM30_04995"
     CDS             complement(2377..>3171)
                     /locus_tag="PLM30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOR54560.1"
                     /translation="VRVAAMPISIDYRSFRDTAKSAKVNRILKELKSQVPWENIAIGV
                     DRLDYTKGIPQRIRAFGEACRSFPELRGKIALIQIVVPSREDVPAYQELREEIEKLVS
                     SVNGEFSTPGWIPIHYMHKSVSREELAAYYRLADIALVTPLKDGMNLVAKEYCACQVD
                     DNGVLILSEFAGAAAQMHRSAILVNSYDIQGMAGAIHEAVQMPLEKRKSRMRRLRAGI
                     RRQNVFWWVDNYLRASTGRALVDFPEARMAPLFHALTKRAPEAPTK"
BASE COUNT          557 a         1009 c         1014 g          591 t
ORIGIN      
        1 gtgtagaacc cgacgggcat ggagtcgaag agttcccggt acttggcctc gctctccttc
       61 aaggcccttt cggcggcctt ccgggcggtg atgtcccggt agatgccgta ggcgaacctc
      121 ctgccgtcgg agaggcagac cgggatgccc aggcaggaga cctggatcag cgagccgtcc
      181 ttccccctgc gggtcgcctc ggcagcgacg gcgcgccccc ctgctgtggc cgcggaaaag
      241 cccctggcct cttccaggta ctccgtgccc ccggcgacga ggtcgtcgat attccgcccc
      301 aggacctctt cgggggcgta cccgaacatg tcgcaaaagg aggggttggc tttcagcacc
      361 gtcagatcgt gatcgcagaa gactatcccc tccatggagt acccgaagag ggcgtccagc
      421 gattccctct cgaaggccat ggcctcctcg gtcctcttgc gctccgtcac gtctctcccg
      481 atggactgga tctcgaccag gttcccccgg ggatcgaaga tcccccggtc ggtccactcc
      541 tgccaccgga tctcgccgtc gggcatgagg gtcctttcca ctccggccag gacggggctc
      601 tccggcgtga ggcctctgag cttctccttg agggatggca tgtcgccggg gtggacatga
      661 tccttgaagc tctttcccac gacatcttcg taccttacgc cgtggtactc gcagtaggtc
      721 ctgttggcga aggtggtgac caggtccggg gtgaacctca ggatgagctc cgggacggag
      781 tccaggatct ccctcaaacc cctctccacc attccggtcc ccttttcccg ttcctcctcg
      841 gcgtcctctt cagggttccg gggggtgatg tccttcctgg gcaaggcatt acctccaaaa
      901 gtaattcatc ggccgataat tatatctccc tttttcaaag gggacaacgg cccgccgcaa
      961 taaacctcag cactgccttt cttcccttcg gggcgccccc tggggtagtg atccttctgt
     1021 tatagtgtgt tcagggcctt tgtcccatcg aaaggtgccg ataaacgacc gaagggagag
     1081 accgttttga agaagaccat cgcggctgtc agggcaatgg agatcctgga ttcaaggggg
     1141 aacccgaccg tccgcacctg gtgcaggctc gacggtggca tcgaggttac cgcctcggta
     1201 ccctccgggg cgtccacggg ggagaacgag gcggtagaac tccgcgacgg cgacatggcg
     1261 cggttcggcg gcaagggcgt cctgcgggcg gtggaaaacg tcaacgcgat cattgccccc
     1321 cgcctcgtag ggatggaccc gacgcaccag gaggagatcg accgtctgat gatcgaactg
     1381 gacggtaccg ctaataaggg cgccctgggc gccaacgcca tccttggggt atccatggcc
     1441 gtggcgaagg cggcctccag ggccagggac ctcccccttt acgcctacct gggcggcccg
     1501 ggggcggcaa ggctgcccgt ccctatgatg aacatcctca acggcggcaa gcacgccgac
     1561 aacagcgtgg acctccagga gttcatggtc atgcccgtcg gggcccccac cttcgccgag
     1621 gccctgcgtt acggcaccga gaccttccac gccctgaaaa agatcctctc gtcgaggggt
     1681 tatgtcacgg ccgtgggcga cgaaggcggc ttcgcgcccg acctggggag caacgacgaa
     1741 gcctgccggg tcatcgtcga ggccatcgag gccgcgggat acacccccgg ccgggacatc
     1801 gccctggcgc tggacccggc ggccagttcc ttctacgaag acggcgccta ccatctggca
     1861 cggtcggggc aaggcaggag gaccagcgac cagatgacgg ccctctatgg tgagtgggtc
     1921 gagaagtacc acgtggtctc catcgaggac gggctcgacg agaatgactg ggagggtttc
     1981 agggcccaga cggccgaact gggtgacagg gtccagatcg tgggggacga cctcttcgtg
     2041 accaacgcca ggttcgtcga gcgtggcatc agggaagggt cggccaacgc cgtcctcata
     2101 aaacttaacc agatcggcac cgtcacggag accgtcgaga cgataaaaat gtgcagggag
     2161 gccgggtggg gttatgtcat atcccaccgg agcggggaga cggaggacac cttcatagcc
     2221 gacctggccg tagctatggg gggcggccag atcaagacgg gctccgcctg ccggagcgaa
     2281 cgggtcgcca agtacaacag gctcctggag atagaggacc aattgggggg cagggcggtc
     2341 ttcaggaacc ccttcgaggg ccgctgaggg cgaggctcat ttcgtagggg cctccggcgc
     2401 gcgcttcgta agggcgtgaa agaggggggc catcctcgcc tcggggaagt cgaccagggc
     2461 cctgcccgtc gaagccctga ggtagttgtc cacccaccag aacacgtttt gcctgcggat
     2521 gcccgccctc aggcggcgca tgcggctctt cctcttttcc aggggcatct ggaccgcctc
     2581 gtggatggcc cccgccatgc cctggatgtc gtaggagttc accaggatgg cgctccggtg
     2641 catctgcgcg gcggcgccgg cgaactcgct cagtatgagt acaccgttat cgtccacctg
     2701 gcacgcgcag tattccttgg cgaccaggtt catgccgtcc ttcagggggg tcaccagggc
     2761 gatatcggcg agcctgtagt acgccgccag ttcttccctg gagaccgact tgtgcatgta
     2821 atggatcggt atccaccccg gcgtggagaa ctcgccgttg acggaactga ccagcttctc
     2881 gatctcctcc ctcagctcct ggtaggcggg gacgtcctcc cggctgggga cgacgatctg
     2941 gatcagggcg atcttccccc tcaattcggg aaaggaccgg caggcctcgc cgaaggctct
     3001 tatcctctgg ggtatgccct tggtgtagtc caggcggtcg acgccgatgg cgatattctc
     3061 ccaggggacc tggcttttca gttccttcag gatcctgttc accttggccg acttggccgt
     3121 atccctgaag gacctgtagt cgatgctgat gggcatggcc gccaccctga c
//
LOCUS       DAOSRG010000128         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_27943_length_3139_cov_8.714008, whole genome shotgun sequence.
ACCESSION   DAOSRG010000128 DAOSRG010000000
VERSION     DAOSRG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27943_length_3139_cov_8.714008"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLM30_05000"
     CDS             <1..748
                     /locus_tag="PLM30_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOR54561.1"
                     /translation="GEALSTFHVRGLSRGCYDIPQGKVVSKDPLGLFSRDKPFGGPQE
                     VLVYPALLDLEDQDFAQRHLMGNMRSNRPFMPDLTRVSSLRDYTPDTPARRIHWKASA
                     RHGKLLAKVLEHSADLRLCIALDGDSFQLPGPSMEKALSLAASLAVWADEKKVPFGLV
                     SNLSRHGRPGPVSLPVGSGPSQPSLALEALARAELPTALSFSVLLSVESRLLPWGTTI
                     IVIRCDGSDDSLIAGGLVTEVFGTYEVSRL"
     gene            745..2046
                     /locus_tag="PLM30_05005"
     CDS             745..2046
                     /locus_tag="PLM30_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOR54562.1"
                     /translation="MTNLLFRMFLVAGESLNFTLLYLYALSGLGAKGYVNPWTFLTMA
                     SSVAAVNLAMGRGNFRYLAIVLANLSVFLASLLFGRGQAGLFSLPGDLQGLMGFWATW
                     TLLAAGGGRAAFLAWTKNPPRYGLFDINMATLFLVLLLASSFGIHLPGVRGLLFLAVL
                     SNVLMLFFGSRGEIPGHGLSRPLPSLAALAALLVPMWVIGHSDALSLLEKPAGSVVEF
                     SKPFFGLLLQALTAVLLAIFRLMKLIPQQQVPADPEGGFAFAEEGLMPSGGSGPFIRY
                     LLWGMAIFIAVLALLGVLYLLWLLLSFLFQKRSGRAEGETWSRVTFRDFLAVLFRAMK
                     RLALLIAVFLPLPIGAGQAYRSLLLWGKSKGSPRRPAETAYEYMDRLIERFPGREREL
                     SRITGSFVRHRYSREGRVFEKGLKWYVLRLYLGRFPLPKGS"
     gene            complement(2068..2898)
                     /locus_tag="PLM30_05010"
     CDS             complement(2068..2898)
                     /locus_tag="PLM30_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR54563.1"
                     /translation="MYKDDTIYYPQDEANTVLLPVTTGCSYNRCAFCSMYKDTKYAPV
                     PFPAIEAELRSGYLYTEKIFLTGADPLSIGYSEMKRILGAIHHYLPYCHRVASYASIR
                     SISRYSLEELSVLHDAGLRLLYIGFESGKDDVLKSMKKGHTVDEAIEQARKLNEARLP
                     FYTVIMYGIAGEGESLRNALSTAAMINRFNTGKVITMNLVVFYGAELDGMVKRGEFTP
                     PGAKERLLEIRTLLESLAPRDRMVFDTTHPTNIIKTYGTLPEDRRRLLVEVDRHLDKA
                     "
BASE COUNT          579 a          894 c          933 g          733 t
ORIGIN      
        1 cggcgaggcc ctttcaactt tccacgtcag gggcctttcc aggggctgtt acgacatccc
       61 ccagggcaag gtcgtctcaa aggaccctct ggggctcttt tcaagggata aaccattcgg
      121 tggcccccag gaggtcctcg tctatcccgc gttgctggac ctggaggacc aggacttcgc
      181 ccagcgccac ctgatgggta atatgcgatc caataggccc tttatgccgg accttacaag
      241 ggtatccagc ctgcgggact acacaccgga tacgccggca cgacggatcc actggaaggc
      301 gagcgcccgg cacgggaaac ttctggccaa ggtgttggaa cattcggccg acctaaggtt
      361 gtgcatagcc ctggatgggg acagcttcca actgcccgga ccgtccatgg agaaggccct
      421 ttcactggct gccagcctgg cggtttgggc cgacgagaag aaggtgccct tcggcctcgt
      481 gtccaacctg tcccgccacg gccggccagg cccggtctcc ctcccggtgg gctccggccc
      541 ttcccagccc agcctggccc tcgaagcgct ggcaagagcg gaactgccta cggcgttatc
      601 cttcagcgta ctcttgtccg tggaatcaag gctcttgccc tggggtacga cgatcatcgt
      661 catacgctgc gatggctccg atgactccct tattgcaggc gggctagtga ccgaagtgtt
      721 tggtacgtac gaggtatcga ggttatgaca aacttgcttt tccggatgtt cctggtcgcc
      781 ggcgagtcgt tgaacttcac cctcctctac ctttacgcgc tatcgggcct cggggcgaaa
      841 ggatacgtga acccctggac ctttctgacc atggcctcat cggtggcagc ggtcaacctg
      901 gccatgggca gggggaactt tcgatacctg gccatcgttc tggccaacct ttccgtcttc
      961 ctggcctccc ttttgttcgg gaggggccag gcgggacttt tttcattacc cggagatctt
     1021 caggggctga tgggtttttg ggccacctgg acccttttgg cggccggggg aggccgggcg
     1081 gccttcctcg cgtggacgaa gaaccctccc cgctatggac tctttgatat aaacatggcg
     1141 accctcttcc tggtgctgct cctggcctcg tccttcggaa tccacctgcc gggtgtgcgg
     1201 gggcttctct tcctggccgt cttgtcgaac gtgttgatgc tctttttcgg ttcccgggga
     1261 gagatccctg gtcacgggct cagtcgaccc ctgccgtccc tggcggcact ggcggcccta
     1321 ttggtgccga tgtgggtgat cgggcattcc gatgcccttt ccctgttgga aaaaccggcg
     1381 gggtcggtgg ttgagttcag taagcccttc ttcggcctcc tgctacaggc actgaccgcc
     1441 gtgctactgg ccatcttccg gctcatgaag ctgatacctc aacaacaggt cccggcagac
     1501 ccggagggag ggtttgcctt cgccgaagaa ggcctgatgc catccggcgg gtcggggcct
     1561 ttcattcggt atctcctctg gggcatggcg atctttatcg cggtgctagc tctcttgggg
     1621 gttctctacc tgctgtggct gctcttaagc tttctcttcc agaaaaggtc cggcagggcc
     1681 gagggtgaaa cctggtctag ggtaaccttc agggatttcc tggccgttct tttccgggcc
     1741 atgaagcgcc tggccctttt gatagcggtc ttcttgccgc ttcctatcgg cgcgggacag
     1801 gcttaccgat ccttgctcct ctgggggaag agcaagggaa gccccaggag gcccgccgaa
     1861 acggcctacg agtacatgga caggctgatc gaaaggttcc ccggccgtga aagggaactc
     1921 tcccgcataa cggggagttt cgtacgtcac aggtattcca gggagggaag ggtcttcgaa
     1981 aagggactga aatggtacgt gctgagattg tacctgggaa ggttccccct tccgaagggg
     2041 agctagattc cggccacttc tgccaggtca ggccttgtcc aggtgcctgt caacctcgac
     2101 taaaagcctt cgtctatcct ctggtagggt gccgtaggtt ttgataatgt tcgtaggatg
     2161 ggtcgtgtcg aagaccatgc gatccctggg ggcgaggctt tccaggaggg tccttatctc
     2221 aagcaggcgt tctttcgccc cgggaggggt gaattctcct cttttgacca tgccgtccag
     2281 ttccgcccca tagaagacca ccaggttcat ggttattact ttacccgtat tgaagcggtt
     2341 gatcatcgcg gcggtggaca gggcgttcct caaggactcc ccttccccgg ctatgccgta
     2401 catgatcacg gtgtagaagg gcaacctggc ttcgttcagt ttcctggcct gctcgatggc
     2461 ttcgtctacg gtatggccct ttttcattga cttgagcacg tcatccttgc ccgactcaaa
     2521 accaatgtag agcagcctga ggccggcgtc atgaaggaca gaaagctcct ccagggaata
     2581 ccgggaaata cttcggatgg aggcgtagga agccaccctg tggcagtaag ggagatagtg
     2641 gtgtatcgcg ccgaggatcc gtttcatttc ggagtaacct atcgacaggg ggtcggcgcc
     2701 cgtcaggaag atcttttcgg tgtaaaggta accgctccgc agttctgctt ctatggcggg
     2761 gaaagggaca ggggcgtact ttgtgtcctt gtacattgag cagaaagcac accggttgta
     2821 ggagcatccc gttgtgacgg gcaaaaggac ggtgttggcc tcgtcctgag gatagtagat
     2881 agtgtcgtct ttatacatag tcccagtata gacctttcac aaagtaatag tcggcaagct
     2941 gcgagtggaa cacccctagg aatcgtaaat cgtaagggcc gtgagtcgta aatcgtaagt
     3001 cgggaaccgt aagaacaaaa caatttgccc gcgaatttga cctttcgatt cacggttcac
     3061 gattcacggt tcacggcact tagaattcat ggcaccatac aaaagggccg gaggcgtacg
     3121 cctccggccc tgggttatc
//
LOCUS       DAOSRG010000129         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_27996_length_3135_cov_11.487987, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000129 DAOSRG010000000
VERSION     DAOSRG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27996_length_3135_cov_11.487987"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1187
                     /locus_tag="PLM30_05015"
     CDS             3..1187
                     /locus_tag="PLM30_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOR54564.1"
                     /translation="MTMLNERQEAVFRGYDDGLIEFARELVRVRSYSGEEEGVARLVE
                     AKMKELGYDEVFIDGTGNVVGRVGGGARSVLFDSHMDTVEVKDEGEWSLPPFGAEIRD
                     GRLYGRGSVDMKSSLAASIYAAAAARDLGLYEGRTVYVTATVSEEYCDGVNLRMLFAE
                     TGLRPGFVVICEPSDNLVTLGHKGKAQLLLRTHGVSAHGSAPEKGVNAVYEMAEIITR
                     VEKLNENLASFGPPHGTIVLSDISCVSASLNAVPSECSIYLDRRLVPGETEEDVKREM
                     DALTVGKRASWEIGILRRKSWTGVELVYTPLHEAWKIDRGHELAKACDRAHEKVFGRK
                     PEKYGFWDFGTNAVTPVSMGIPTIGFGPGDYKLAHSVNENCEVDSILEAFRFYGTLIG
                     EV"
     gene            1219..1554
                     /locus_tag="PLM30_05020"
     CDS             1219..1554
                     /locus_tag="PLM30_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOR54565.1"
                     /translation="MITYEDFEKVDMRVGKVIYVQDFPRAKNPSYKVRIDFGGEIGVK
                     SSSLQAKKDYTLEEMIGRYVVCVVNFPPRNIAGFLSEVLVLGVPGLGDSVSLLAVDRA
                     PVPLGGRVF"
     gene            1608..3011
                     /locus_tag="PLM30_05025"
     CDS             1608..3011
                     /locus_tag="PLM30_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR54566.1"
                     /translation="MKKEKGKISAIMREQALDKGLLEKAGRCAQDYLDSVLGRPVFPE
                     EKDLEGLEEFRHDLPEEPGDPGEILDRLHRFGSPGTVAQVGGRYFGFVNGGVVPASLA
                     VKRLIDAWDQNAALYAMSPVVSVLEEVCEGWLVDLFGLPEGSAAGFIGGSATANLCGI
                     AAGRDHLLAQCGWEASSKGLFGAPELKVVVGDGAHATVFKALSILGLGRERLVRVPTD
                     GQGRLRPDGLPALDERTLLILQAGNVSSGAFDPFAEICPRAREAGAWVHIDGAFGLWA
                     AASPRLRHLTAGMELADSWSVDAHKTLNAPYDSGIVLCRRREALTGALQVSGSYIAPS
                     GHRDGMHYTLDMSRRARAVELWACLAAMGQRGVAELVEDLHDKAVLFAHALAREGFHI
                     LNEVCFNQVLVSCGDPRLTEKTLALVQGSGECWCGSAQWDGEFVMRVSVCSYRTTAED
                     IERSVRAFVRAREEARL"
BASE COUNT          565 a          997 c         1053 g          520 t
ORIGIN      
        1 tgatgacgat gctgaatgaa aggcaagaag cggtattccg gggatacgac gacggcctta
       61 tcgaattcgc ccgcgaactg gtacgggtcc ggagctactc cggggaggag gagggcgtgg
      121 cccggctggt ggaggcgaaa atgaaggagc tgggctacga cgaggtcttc atcgacggga
      181 ccggcaacgt ggtgggccgc gtcggcggcg gggcgaggag cgtcctcttc gactcccaca
      241 tggacaccgt ggaggtcaag gacgaagggg agtggtcact gccccccttc ggcgccgaaa
      301 taagggacgg ccgcctctac ggccgcggct cggtggacat gaagtcctct ctggccgcct
      361 ctatctatgc cgccgccgcg gcccgggacc tgggtcttta cgaagggcgg accgtctacg
      421 tgaccgccac cgttagcgag gagtactgcg acggggtgaa cctcaggatg ctcttcgccg
      481 aaacggggct gaggcccggt ttcgtcgtca tctgcgagcc ctcggataat ctggtgaccc
      541 tggggcacaa gggcaaggcc caactgctcc ttcgcaccca cggcgtgtct gcccacggct
      601 ccgcccccga aaagggcgtc aacgctgtct acgagatggc cgagattatc acccgagtgg
      661 aaaaattgaa cgagaacctg gcctctttcg gtccgcccca cggaacgatc gtcctctcgg
      721 acatatcctg cgtcagcgcc tccctcaacg ccgtgcccag cgagtgcagc atctacctcg
      781 acaggaggct ggtccccggt gagaccgaag aggacgtgaa gcgcgagatg gacgccctga
      841 cggtcggcaa gagggcctcc tgggagatag gcatcctccg gaggaagagc tggaccggcg
      901 tcgaactggt ctacaccccg ctccacgagg cctggaagat cgacaggggc cacgaactgg
      961 ccaaggcctg tgacagggcc cacgaaaagg tcttcgggcg aaaaccggag aagtacggct
     1021 tctgggactt cgggaccaac gccgtgaccc ccgtgagcat ggggataccc accatcggct
     1081 tcggccccgg cgattacaag ctggcccaca gcgtcaacga gaactgcgag gtggacagta
     1141 tcctggaggc cttccgcttt tacggaacgc tgatcgggga ggtatgaacc cgtttcatcg
     1201 acgaaggggg acgaaacagt gataacctat gaggatttcg aaaaggtcga catgcgcgtc
     1261 ggtaaggtga tctacgtcca ggattttccc agagcgaaga atccctccta caaggtcagg
     1321 atcgacttcg gcggggaaat aggcgtgaaa agctcgagcc tccaggccaa aaaggactac
     1381 accctcgagg aaatgatcgg ccgctacgtg gtctgcgtgg tcaacttccc ccccaggaac
     1441 atcgcggggt tcctgtcgga ggtgctcgtc ctgggcgtcc ccggcctggg ggacagcgtc
     1501 agcctgttgg ccgtcgacag ggcccccgtc cccctcggcg gcagggtttt ctagtcacga
     1561 cgggtgactg gatatcaggg ggacagggct ccccgggagg tcaaaggatg aaaaaggaaa
     1621 agggcaagat ctccgcgata atgcgcgaac aggctctcga caagggattg ctcgaaaagg
     1681 ccgggaggtg cgcccaggat tacctggact ccgtcctcgg ccggcccgtc ttcccggaag
     1741 agaaggacct cgaagggctc gaggagtttc gccacgacct gcctgaagag cccggcgacc
     1801 cgggggagat cctggacagg cttcaccgtt tcggatcgcc cgggacggtc gcccaggtgg
     1861 gggggcgcta cttcggtttt gtcaacgggg gggtcgtccc cgcctcgctg gcggtgaaac
     1921 ggctcatcga cgcctgggac cagaatgccg ccctctacgc gatgtccccg gtcgtgtcgg
     1981 tcctggaaga agtctgcgag gggtggctcg tggacctctt cggccttccc gaaggctccg
     2041 ccgccggttt catcgggggc tccgccacgg ccaacctctg cggcatcgcg gccggccgcg
     2101 atcacctcct ggcgcaatgc ggctgggagg cctcatcgaa agggcttttc ggggcacccg
     2161 agctcaaggt ggtcgtcggc gacggggcgc acgccacggt cttcaaggcc ctctcgatcc
     2221 tcgggctcgg cagggagcgc ctcgttcggg tccccaccga cgggcaggga cgcctaaggc
     2281 ccgacggcct acccgctttg gacgaaagga cgctgctgat actccaggcg gggaacgtca
     2341 gctccggggc cttcgacccc ttcgccgaga tctgccccag ggccagggag gcgggtgcct
     2401 gggtccatat cgacggggcc ttcggcctct gggcggcggc gtcgccgagg ctcaggcacc
     2461 tcacagctgg aatggaactg gccgattcgt ggtccgtcga cgcccacaag accctcaacg
     2521 cgccctacga ctccggcatc gtgctctgcc gtcgaaggga ggccctcacc ggggccctcc
     2581 aggtgtcggg ttcctacatc gcccctagcg gccatcgtga cgggatgcac tacaccctcg
     2641 acatgtcgag gagggcgcgg gccgtggaac tatgggcctg cctggcggcg atgggccagc
     2701 ggggagtggc cgaactggtc gaggacctcc acgacaaggc ggtcctcttc gctcacgccc
     2761 tggcgaggga gggtttccat atcctcaacg aggtctgctt caaccaggtg ctggtctcct
     2821 gcggcgaccc ccgcctcacc gaaaagaccc tcgccctggt ccagggttcg ggggaatgct
     2881 ggtgcggcag cgcccagtgg gacggcgagt tcgtgatgag ggtcagcgtc tgctcctacc
     2941 gcaccacggc cgaggacatt gaacggtcgg tgcgggcctt cgtccgtgcc cgggaggaag
     3001 cccgcctctg accggggccc caggctgctg gaggagcccc ggtcatctcc ggcttcaccc
     3061 ttgcttaccc ctttcgagcc ctgatcccgg cggcgatcga gtcccccgcc gccgttgtcg
     3121 tggccttccc cccca
//
LOCUS       DAOSRG010000130         3133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28013_length_3133_cov_10.832684, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000130 DAOSRG010000000
VERSION     DAOSRG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_3133_cov_10.832684"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLM30_05030"
     CDS             <1..317
                     /locus_tag="PLM30_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54567.1"
                     /translation="EFARRRAIITDLLKEMPLVSFSEPQGAFYVFLNVAGALGGLFNG
                     KKLEDDATFCEALLETEYVAAVPGAAFLAPGHIRLSYANSEAEIREGMARLKRFLGSI
                     EV"
     gene            complement(372..929)
                     /gene="frr"
                     /locus_tag="PLM30_05035"
     CDS             complement(372..929)
                     /gene="frr"
                     /locus_tag="PLM30_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOR54568.1"
                     /translation="MADQEIRKMKERMEKAVEHLKKELLGIRTGRAHPALVSDIKVDY
                     YGNPTPMSQVGSISTPDARQILITLWDKNAVKAAEKAIQASPLGINPSVDGENIRLVI
                     PELTGERRVELTKLVKKCAEDARIAVRNLRRESNEVLKKMEKDGSISEDSMHDHLEMI
                     QKATDDFVKRIDVIAAEKEKEVLED"
     gene            complement(932..>1543)
                     /gene="pyrH"
                     /locus_tag="PLM30_05040"
     CDS             complement(932..>1543)
                     /gene="pyrH"
                     /locus_tag="PLM30_05040"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOR54569.1"
                     /translation="LTKNGLEIAMVVGGGNIFRGARSDEEYLDRAQGDYMGMLATVIN
                     ALALQSVLERLGVPTRVQTAIEMRELAEPFIRRKALRHLEKGRIVIFAAGTGSPFFSS
                     DTAAALRAAEISADCLLKATKVDGIYDKDPAVNPDARLLPRVTYAEALQKQLKIMDAA
                     AFSLCMENRIPILVANILKEGTLRDILVLGREAGTVVHFEKEC"
     assembly_gap    1544..1643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1644..2350)
                     /gene="rpsB"
                     /locus_tag="PLM30_05045"
     CDS             complement(<1644..2350)
                     /gene="rpsB"
                     /locus_tag="PLM30_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOR54570.1"
                     /translation="MAVVSMKQLLECGVHFGHQTRRWNPKMKPYIFTERNGVYIVDLQ
                     KTVKGLEKAYDFLREVSKNGGSVLFVGTKKQAQETVREEALRCGQYYINHRWLGGLLT
                     NFQTIKKRVTRMVELGSPEFLEGSTKWTKKEIAGFEKERAKLEKFLIGIKEMRDVPDA
                     LVVVDPKREDIAVLEARKLGIPITGIVDTNCDPDLIDYPIPGNDDAIRAIKLIVGLLA
                     SAVIEGRQGVDNLVEESA"
     gene            2576..2887
                     /locus_tag="PLM30_05050"
     CDS             2576..2887
                     /locus_tag="PLM30_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOR54571.1"
                     /translation="MSLIERIDNMPPQQKAMLNRLKRVEGQLRGIQRMIINEKSCQEI
                     LLQLSAARKAMQNACIEILKGYVRKCLAESGTPDMDELERLISTLIDIAPITGETIEG
                     S"
     gene            complement(2900..>3133)
                     /locus_tag="PLM30_05055"
     CDS             complement(2900..>3133)
                     /locus_tag="PLM30_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54572.1"
                     /translation="RLPVNLSRRRAVMIAEGAAVTEGREGWRSLAGSCHVLAREEKAL
                     ACWKAWIRSASFLIDSTSGKKVTLGEMLGLLLP"
BASE COUNT          613 a          899 c          805 g          716 t
ORIGIN      
        1 atgagttcgc ccggcggcgc gcaatcatta cagacctcct taaggagatg cccctggtct
       61 ccttcagtga accccagggg gctttctatg tattcctgaa cgtcgccggg gcccttgggg
      121 gactttttaa cgggaagaaa ctggaggatg atgccacctt ttgcgaggct ctccttgaaa
      181 cggagtacgt cgcggcggtg ccgggggccg ctttcctggc tcccgggcat atccgcctga
      241 gttacgccaa ctccgaggct gagatccggg aggggatggc caggttgaaa agattcctcg
      301 ggtcaataga agtatagtaa caaggggacg tcccacccga gggacgcccc cttcgctgaa
      361 aatagatcac gtcagtcttc caatacttct ttctcttttt cggcggcgat gacgtcgatc
      421 ctcttaacga aatcgtcggt ggccttctgg atcatttcca ggtgatcgtg catggagtcc
      481 tcgcttatac tgccgtcctt ttccatcttc ttcagtactt cgttcgattc acgcctcaga
      541 ttcctaacgg cgatcctggc gtcctcagcg cacttcttga cgagcttggt gagctcaacg
      601 cgcctttcgc cggtcaactc ggggatgacc agcctgatgt tctcgccgtc caccgatggg
      661 tttatcccca agggcgacgc ctggatcgcc ttctccgcgg ctttcactgc gttcttgtcc
      721 caaagggtta tgagtatctg cctcgcgtcg ggtgttgaaa tggaacccac ctgggacata
      781 ggtgtcggat taccgtaata gtcgaccttg atatcactca cgagggccgg gtgggctctc
      841 ccggtcctga tgcccaggag ttcctttttc agatgctcga cggctttttc catcctttcc
      901 ttcatcttcc tgatctcttg atcagccata ttcaacactc cttttcgaaa tggacgaccg
      961 taccggcttc tctgccaagg accaggatgt cccggagtgt tccctccttg aggatgtttg
     1021 ctaccagaat ggggatccgg ttctccatac agagcgaaaa agcggccgca tccatgatct
     1081 taagctgttt ctgaagcgct tcggcatagg tgacccgggg aagcagcctc gcatcggggt
     1141 tgaccgccgg gtccttatca tatataccat cgacctttgt ggccttcaac aggcagtcgg
     1201 cggatatctc ggccgccctg agcgccgcgg cggtgtcgga ggaaaagaag ggagagcccg
     1261 taccggcggc gaagatgact atcctcccct tttcgaggtg ccggagagct ttcctccgga
     1321 taaagggttc ggccagttcc ctcatctcga tagccgtctg gacccttgtg gggaccccaa
     1381 ggcgctccag cacgctctga agggccagtg catttattac cgtggctagc atgcccatgt
     1441 aatcaccctg cgccctatcc aggtactctt cgtccgaccg ggcgccccgg aatatattgc
     1501 cgccgcccac caccatggct atctccaggc cattcttcgt cagnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnngcgctct cttcgacgag gttgtcgaca ccctgccgcc
     1681 cttcgatgac ggccgaggca aggaggccaa cgataagttt gatggccctg atggcgtcat
     1741 cgttaccggg gatcgggtaa tcgatcaggt cgggatcaca gttggtgtcc actatcccgg
     1801 ttatgggaat gccaagcttt cgggcctcga ggaccgcgat atcctccctc ttgggatcaa
     1861 cgacgacaag ggcatcgggg acatcgcgca tttccttgat cccaatcagg aacttttcaa
     1921 gtttcgcccg ttccttttca aagccggcga tctctttctt ggtccacttg gtcgatccct
     1981 ccaggaattc gggggaaccg agctccacca tgcgggtgac tcttttcttg atcgtttgaa
     2041 agttggttag aaggccaccg agccaacggt ggtttatgta gtactgcccg cagcggagag
     2101 cttcctccct tactgtctcc tgggcctgct tcttggtccc tacaaagaga acgctccccc
     2161 cgttttttga aacctcccga aggaaatcat aggccttttc gagaccctta acggtctttt
     2221 gaaggtccac gatgtagacg ccgttccttt cggtgaaaat ataaggcttc atcttggggt
     2281 tccagcgcct tgtctggtgt ccgaagtgca ccccacactc cagcagttgc ttcatgctta
     2341 ccactgccaa tttttccgcc tccctggttt tggttttccc tccgcgccgg tcatccgaaa
     2401 gacgggaccc gcatgcctgc ggacaccacc gtttccgtcc cgatcgcgtg cgtgataagg
     2461 gccaacgggg agtatatcac agttgtcgat tgaccgacaa caatttgtgg aataccatat
     2521 acccgtcagg tctacttgtt gtccaaaaaa gcggcggaga aaggggaaga aattaatgtc
     2581 actgattgaa aggatcgata acatgccccc ccagcaaaaa gccatgctga acaggctaaa
     2641 gagggtcgag ggccagcttc gcgggataca gaggatgatc attaacgaaa agtcctgcca
     2701 agagatcctg ctgcagctat ccgccgctag gaaggccatg cagaacgcct gcatcgagat
     2761 actcaagggc tacgtcagga agtgcctcgc cgagtccggc acacccgaca tggacgaact
     2821 ggaaaggctc atttccacac tgatcgacat agccccgatc accggtgaaa caattgaggg
     2881 gtcctgaagg ttacttcgcc tagggcagca acaagccaag catttctccg agggtgacct
     2941 tcttcccgct ggtcgaatcg ataagaaagc tggcggatcg gatccatgct ttccagcagg
     3001 caagggcctt ctcctcccgt gccagaacat ggcatgaccc cgcgagggac ctccaacctt
     3061 cccttccctc ggtcaccgcg gccccctcgg cgatcatcac cgctctcctg cgggagaggt
     3121 tcacgggaag cct
//
LOCUS       DAOSRG010000131         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28186_length_3117_cov_12.432724, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000131 DAOSRG010000000
VERSION     DAOSRG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28186_length_3117_cov_12.432724"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..861
                     /gene="prxU"
                     /locus_tag="PLM30_05060"
     CDS             145..861
                     /gene="prxU"
                     /locus_tag="PLM30_05060"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930177.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:394..396,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOR54573.1"
                     /translation="MEEKEIGCARPTGALVGEPEPAPEEKAEPAKKEVQGMTIMVGRK
                     APDFEAPVYHKGKFGSVKLSDSLGKWVVLCFYPGDFTFVUATEVSAVADKYPVFQELG
                     VDVYSVSVDSQFVHKIWNDNELSKMVEGGVPFPMISDAGGNLGKIYGVYDEKAGVDVR
                     GRFIIDPDGVIQGMEVLTPPVGRNVEETIRQVKAFQLVRETKGKEVTPSGWQPGKTTL
                     KPGPDLVGNVWKFWKPRDNK"
     assembly_gap    1171..1270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1271..1696
                     /locus_tag="PLM30_05065"
     CDS             <1271..1696
                     /locus_tag="PLM30_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR54574.1"
                     /translation="LWAVEVPLDRRDPEFVNPPSRLVVPDGEGWRPDPFPDDISMAVK
                     GERGLSVILGCAHAGVVNILEEVSAHFGTRDFHMVLGGMHLGDQTPEFVDRVTSELVS
                     RFNVEKWRPCHCTGLKALCVLSAKAGDVAWAAAGTVLEV"
     gene            complement(1707..2387)
                     /locus_tag="PLM30_05070"
     CDS             complement(1707..2387)
                     /locus_tag="PLM30_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR54575.1"
                     /translation="MMTRILIVEDDPLVLELHRQFVSTMEGFTVAGTATGGSQALEFL
                     ENNPVDCLLLDIFMPDFDGLELLKRMRERTVDTDVIIISAANEGDKVKEALRLGAFGY
                     LLKPFRYERLRSMLCALKEHQGEIRRKKVVGSQEDIDRAFDPGLKAADTRLSYPKGIQ
                     ENTLDLVTAALKSMGKPSSAAEIAESSGISRVTVQRYINFLKRTGRILEEVEYQKTGR
                     PLKRYSLI"
     gene            complement(2389..>3117)
                     /locus_tag="PLM30_05075"
     CDS             complement(2389..>3117)
                     /locus_tag="PLM30_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54576.1"
                     /translation="RSGNRILGAIASFRKADEIQTLAEELTGARKLAEVLKVQNELLR
                     VHKHEYLNKLHAISGSIQLGDWDSALGLIHSESDSQQEMIGKIHETIRQPEIAGLLIG
                     KISRGRELGVDLELDYRSSLKEHPSVDTNSLIVCLGNLIENATEAVQEPSVPGKKVTV
                     LLEHTDKELVVEVTDTGVGIPAEDRPYLFEKAFSNKAGGKRGYGLFLVRSVVLSYGGT
                     IEFESTVGEGTRFRVVFPEEGGPA"
BASE COUNT          565 a          898 c          945 g          609 t
ORIGIN      
        1 gcacatggcc gggtgagcgg tgggaacgga cgttcccctg tggctgcgtc ctccactaaa
       61 cctgaagaat atgctatcat attacctgta aatgatttcc agaattattt caggttgaaa
      121 atctgacatc gggaggtgtg ttccttggag gaaaaggaga tcggctgcgc ccgcccgacg
      181 ggagcgctcg tgggtgagcc ggaaccggca ccggaagaga aagcggagcc agcaaagaag
      241 gaggtacagg gtatgacgat catggtggga aggaaagccc cggatttcga ggcccccgtt
      301 taccacaagg gcaagttcgg cagcgtcaag ctgtccgatt cgcttgggaa atgggtagtg
      361 ctctgcttct accccggtga cttcaccttc gtctgagcga cggaagtgtc ggcggtcgcc
      421 gacaaatacc ccgtattcca ggaactggga gtggacgtct actccgtgag cgtggacagc
      481 cagttcgtcc acaagatctg gaacgataac gaactttcaa agatggtaga aggcggagta
      541 cccttcccga tgatctccga cgcgggaggc aacctcggca agatctacgg cgtctacgat
      601 gaaaaggccg gcgtcgatgt gcggggccgt ttcatcatcg accccgacgg cgtgatccag
      661 ggcatggagg tcctgacccc tcccgtgggg aggaacgtcg aggagaccat ccgccaggtc
      721 aaggccttcc agctggtccg cgagaccaag ggcaaggagg tcaccccttc ggggtggcag
      781 cccggcaaga ccaccctcaa gcccggcccc gacctggtcg gcaatgtctg gaagttctgg
      841 aagcccaggg acaacaagta gcacgtcgaa ggacgggtcg cccccaaaaa gggggcgact
      901 ttttttatcc gccgctggcg gcccccggcg caaggcgcta cggggagtga caccggggga
      961 gcctgggtct atccttgaag ggggctcggt attcgggccc tggctggtga aatgaaaaag
     1021 atcggggtgt tgagatgaag atcgcggtga tcgtggagaa cttcgccggt tcatggaaga
     1081 tgatggcgga acacgggctg gccgtcctcg ccgttgacgg cgggacctcg gtgctcatgg
     1141 acacggggca gggcggggac gcgcttttga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn ctctgggccg tcgaggtccc cctggaccgc cgtgacccgg agttcgtgaa
     1321 cccgccctcc aggctggtcg tccccgacgg cgaggggtgg agaccggacc ccttccccga
     1381 cgatatatcc atggcggtga agggagaaag gggcttaagc gtcatcctcg gctgcgccca
     1441 tgccggcgtc gtgaacattc tcgaagaggt ctcggcccat ttcggtacca gggattttca
     1501 catggtgctg ggagggatgc acctggggga ccagacgccc gagttcgtgg accgggtcac
     1561 ctcggaactg gtgagccgct tcaacgtcga gaagtggcgc ccctgccact gcacggggct
     1621 caaggccctt tgcgtcctct ccgccaaggc cggggacgtg gcctgggccg ccgcgggaac
     1681 ggtcctcgag gtatgaacgc cccggttcag atcagggaat agcgcttgag gggcctcccg
     1741 gtcttctggt actcgacctc ctccaggatg cgcccggtcc tcttcaggaa gttgatgtac
     1801 ctctggaccg tcacccttga aatgccggag ctttcggcga tctcggcggc cgaggagggc
     1861 ttccccatgg atttgagcgc cgctgttact aggtccaggg tgttctcctg tatgccctta
     1921 gggtaggaga gtcttgtgtc cgctgccttc aaacccgggt cgaaggcccg gtcgatatct
     1981 tcctgggagc cgacgacctt tttccgccgg atctccccct ggtgctcctt gagagcgcag
     2041 agcatggacc tcaacctctc gtaccggaag ggcttgagga ggtagccgaa ggcccccagc
     2101 ctgagcgcct ccttgacctt gtccccctcg ttggcggcgg aaatgatgat cacgtcggta
     2161 tcgacggtcc tctccctcat ccttttcaac agttccaacc cgtcgaaatc gggcatgaag
     2221 atgtccagca gcaagcagtc cacggggttg ttctccagga attccagggc ctggctgccg
     2281 ccggtggcgg tgccggcgac ggtgaaaccc tccatcgtcg agacgaattg cctgtgcagt
     2341 tcaaggacca gggggtcgtc ctcgactatc agtatccggg tcatcatttc aggctgggcc
     2401 cccttcctcg gggaaaacga ccctgaaacg ggtcccttcc cccacggtcg attcgaattc
     2461 tatcgtccca ccataggaca gcacgacgga tcttacaagg aaaagcccgt accctctttt
     2521 gccccccgcc ttgttcgaga aagccttttc gaagaggtag ggcctgtctt cagccgggat
     2581 gccgacgccg gtgtcggtca cttcgacgac cagctccttg tcggtgtgct cgagtaacac
     2641 cgttaccttc ttaccgggca ccgacggctc ctggaccgct tcagtggcgt tctcgatcag
     2701 gttcccgagg cacacgataa gagagttggt atcgaccgaa gggtgttcct tcagggagct
     2761 tcgatagtca agttcgaggt ccacgcccag ttccctgccc ctgctgatct tacctatcag
     2821 caaccccgca atctccggtt gcctgatggt ctcgtggatc ttcccgatca tctcctgctg
     2881 ggagtcggac tcgctgtgga tcagccccaa ggctgaatcc cagtccccga gctggatcga
     2941 cccggagatg gcgtgaagct tgttcaggta ttcgtgcttg tggacccgga gcagttcgtt
     3001 ctggaccttg aggacctcgg ccagtttcct ggcgcccgtc agttcctcgg cgagggtctg
     3061 gatctcgtcg gccttccgga aactcgctat ggcccccagg atcctgttcc cggaccg
//
LOCUS       DAOSRG010000132         3114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28213_length_3114_cov_6.849624, whole genome shotgun sequence.
ACCESSION   DAOSRG010000132 DAOSRG010000000
VERSION     DAOSRG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28213_length_3114_cov_6.849624"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1228)
                     /locus_tag="PLM30_05080"
     CDS             complement(251..1228)
                     /locus_tag="PLM30_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOR54577.1"
                     /translation="MGKGRAKRAERVSWIGLWVNAALTAFKFFAGFAGNSAAMTADAL
                     HSLSDLATDAVAVVGFRVTGRPVDASHDYGHGKFETLVTVIVGVSLMAVAVGIFIGGG
                     ERVLAVVRGRAILRPGMIALVAAALSIVVKEALFVFTRRAARSLSSDALMAKAWDHRS
                     DALSSIGTLAGISGALFLGPKARILDPLAALVVAAVIVRVALPVTHRGVSELLEGSLP
                     EEEEGEIIKAILGVPGVRGAHHLRTRRIGSGIALEVHILVDRTLDIASAHDISTAVEA
                     AVRELYGGGAFVSVHVEPVSEDLPEPAPYDDGHNGREEGPGHHDGDFTV"
     gene            complement(1320..2516)
                     /gene="trpB"
                     /locus_tag="PLM30_05085"
     CDS             complement(1320..2516)
                     /gene="trpB"
                     /locus_tag="PLM30_05085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR54578.1"
                     /translation="MKSVSKEVEARKGYFGTFGGSYVPEQLQPRLDELEAAFETAKGD
                     ELFGKEFARLLKDYVGRPSALTHCANVSREYGGGRLFLKREDLNHTGAHKINNTIGQA
                     LLAKRMGKERIIAETGAGMHGTATATVAALMGMKCTVYMGEEDVRRQAPNVSRMKVMG
                     AEVVPVTHGQRTLKEAVDAALDAYVADPGTFYMLGSVVGPHPFPSIVRYFQSVIGKEA
                     RGQFLEREGRLPDHVLACVGGGSNAMGIFSGFLEDGDVKLIGVEPAGRGLDTKDHAAT
                     LSVGKPGVIHGFRSYVLTDDDGEPAPVYSIAAGLDYPGVGPEHAYLLESGRASYVSVT
                     DKEALDAFSVLCRLEGIIPALESSHALAHALKMLPRLSKDETVLVNLSGRGDKDLDTI
                     LPLLAL"
     gene            complement(2926..>3114)
                     /locus_tag="PLM30_05090"
     CDS             complement(2926..>3114)
                     /locus_tag="PLM30_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOR54579.1"
                     /translation="MPTLAAIRCNPVIKSFYERLLEKGKKKMVAITACMRKMLHIMYG
                     IMKNKAYFDPALCMPNVR"
BASE COUNT          569 a          995 c          938 g          612 t
ORIGIN      
        1 gcggttgatc tcctccagcg tctcctggct gatgcgaaac atctcgctca acattctcat
       61 ccccccggtc ctgtatttca cttgaaaaac ccattacttc attatcatat cgtaacccgg
      121 cccgatgaga caacagcctc accggaacag ggaaaccgcc cccttttctg tcaggcccct
      181 ctcccggccc gcagggtgtt cccggtcggc cacgacctga ccggcgccaa cgagggcggg
      241 gcacctcacg ctacacggtg aaatcgccat cgtggtggcc ggggccttct tccctgccgt
      301 tatgaccgtc gtcgtaaggt gcaggttcgg gcaggtcctc ggagacgggt tcgacgtgga
      361 ccgagacaaa ggcgccgccg ccgtagagtt ccctgaccgc ggcctcgacg gccgtcgaga
      421 tatcgtgggc ggaggctata tccagcgtcc ggtcgaccag gatatggacc tcgagggcta
      481 tcccggagcc gatgcgcctg gtcctgagat gatgggcccc cctcaccccg gggacaccaa
      541 ggatggcctt gatgatctca ccttcctcct cttcggggag ggagccctca agcagttcgc
      601 tcacgccccg gtgcgtgacg ggcagggcga cccgaacgat gacggccgcc accacgaggg
      661 cggcaagggg gtccaggatg cgagccttcg gcccgaggaa gagggcgccg ctgatgccgg
      721 ccagggtccc tatcgaggag agggcgtccg accggtggtc ccaggccttg gccatcaggg
      781 cgtcgcttga aagggagcgg gcggccctcc tggtgaagac gaagagggcc tccttcacga
      841 cgatggaaag ggccgcggcg acgagggcta tcatccccgg ccggaggatg gcccttcccc
      901 tcacgaccgc caggaccctt tcgccgccgc cgatgaagat gccgacggcg acggccatga
      961 gggagacccc tacgatcacc gtgacgaggg tctcgaactt gccgtggccg taatcatggg
     1021 aggcgtcgac aggccggccg gtgaccctga aacctacaac cgcgacggca tcggtggcca
     1081 ggtccgacag ggagtgcagg gcgtcggcgg tcatggctgc gctgttgccg gcgaagccgg
     1141 cgaagaactt gaaagccgtc agcgccgcgt tgacccacaa acctatccag gagacccttt
     1201 cagctctttt cgccctgccc tttcccatgg gaagccgcga tcccccgctt tcgcccggta
     1261 atttcagggt tattctaccc cactcggggg gaacgcggct cccttcgctg caagcgctcc
     1321 taaagggcca ggagcggcag gatcgtgtcg aggtccttgt cgcctctccc ggaaaggttc
     1381 accaggaccg tctcgtcctt cgagaggcgg gggagcatct tcagcgcgtg ggccagcgcg
     1441 tgggaactct ccagggccgg tatgatgccc tccagccggc agaggacgct gaaggcatcc
     1501 agggcctcct tgtcggtgac ggagacgtag gaagccctgc cgctttccag caggtaggcg
     1561 tgctcgggcc ccacgccggg gtagtccagc ccggcggcga tggaatagac cggcgcgggc
     1621 tcgccgtcgt catcagtcag gacgtaactg cggaaaccat ggatgacgcc cggcttcccc
     1681 actgagaggg tggcggcgtg gtccttcgtg tcgagccccc tgccggcagg ctccacgccg
     1741 atgagcttca catcgccgtc ctcgaggaaa ccggagaaga tgcccatggc gttgctcccc
     1801 ccgccgacgc aggccagtac gtggtcgggg agcctgccct ccctctccag gaactgcccc
     1861 ctggcctcct tgccgatgac gctctggaaa tacctgacga tcgaggggaa ggggtggggt
     1921 cccacgacgg agcccaacat gtagaacgtc ccggggtccg ccacgtaggc atcgagggcc
     1981 gcgtcgacgg cctccttcaa ggtcctctgg ccgtgggtca cggggaccac ctcggcaccc
     2041 attaccttca tccgcgacac gttgggggcc tgcctgcgga catcctcctc gcccatgtag
     2101 acggtgcact tcatacccat cagggccgcc accgtggccg tggccgtccc gtgcatgccg
     2161 gccccggtct cggcgatgat cctctccttg cccatccgct tggccagcag cgcctggccg
     2221 atggtgttgt tgatcttgtg cgccccggtg tggttcaggt cctcccgctt caggaaaagc
     2281 ctcccaccgc cgtattcacg ggagacgttg gcgcagtggg tcagcgcgga gggcctgccc
     2341 acgtaatcct tcaaaagcct ggcgaattcc ttgccgaata attcatcacc cttggccgtc
     2401 tcaaaggcgg cttccagttc gtccagtctg ggctgcagct gttcgggcac gtaactgcct
     2461 ccgaaagtac cgaagtaacc ctttcttgcc tcgacttcct tgctgacgct cttcatttcc
     2521 tcttcctcct cggctaaaaa atttgggggt cgtccgcaaa caaaaagggg cgttcatcct
     2581 tttatggata cgcccctcgt ttcagaataa aaaacgcggg gtacacggtc gagaccgcgc
     2641 accccgcgtg atatcacgcg tcgggtcgcg gcgccttagc gccaccacca gttgcgatta
     2701 atagacttca tggaacctct gctgaactcg gctggtctca tctgcggaca ctccctctag
     2761 gtagggaatg tatcagattt ctttgggcaa tgcaaggggg ctggggatcg gagatcggaa
     2821 agggaaggcc gaaaggcaaa agcatctcac cacagagata ccgtgttgca agtcaagtat
     2881 tgttttgaac cttgacaatc tggttgtaat cttttcagga ctatttcatc gaacattcgg
     2941 catacagaga gcagggtcga agtatgcctt gttcttcatg attccgtaca taatatggag
     3001 cattttcctc atgcaggccg ttattgcaac catctttttc ttgcccttct ccaggagcct
     3061 ttcatagaag ctttttatta cagggttgca ccttattgct gcgagtgtcg gcat
//
LOCUS       DAOSRG010000133         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28327_length_3105_cov_11.291148, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000133 DAOSRG010000000
VERSION     DAOSRG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28327_length_3105_cov_11.291148"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1299
                     /locus_tag="PLM30_05095"
     CDS             64..1299
                     /locus_tag="PLM30_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOR54580.1"
                     /translation="MNAGDVKKAVKAREREIVSFLSKVLQTPSVTGYEAAVAEVIARE
                     IEGIGLRPRIEEAEKGRPNLLAEWEGLPGPRFVFNGHMDVFPPPAGSEEEYGGPWSGR
                     VEGGWIFGSGANDMKAGLCGSIMAVRVLKEMGFVPKGSVLLTCTCDEENGGWLGVKHL
                     LGKGLIKGDLGLNMEPTSLKLLLDHSGIFRARFIIEGKGAHASNPSEGIDALEKSIGV
                     IQGLYELGKRLEKRSIPGWGSPTLNVTTLHAGTATNVIADRSTFSIDRRLVPGESHDE
                     AEAEIMAVLEEQRKKDPDLRLVREIISDRPALEIPPDSAIVKAIEKGFKDLQGKPLER
                     MKARFGSDAADIVKATGMPMPILGPGNEDEATSVGEKLSIEEYLAAVEIYALTVARLL
                     STRAGSDPHGSSPRASSTT"
     assembly_gap    1547..1646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1647..2062)
                     /locus_tag="PLM30_05100"
     CDS             complement(<1647..2062)
                     /locus_tag="PLM30_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR54581.1"
                     /translation="MCTAPLCFASDYAFTLDESGVPALGGERSVIVVISDIHLGMDDR
                     YAEIQRNRAPLADFLRKVRCAPNIKELVVAGDLVDEWFIPAGTDTYAGKTQKEFVQRV
                     AANNKDVVDAFNDIIRDGTIKVVYVPGNHDLLISSE"
     gene            complement(2338..2619)
                     /locus_tag="PLM30_05105"
     CDS             complement(2338..2619)
                     /locus_tag="PLM30_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54582.1"
                     /translation="MREVIKKSDELIEVGGKEYPLWEIIREGGKLYCREVAIMAEIDG
                     MKIPATLESDDWMRAGRLRQKGDEESLRELEKMEGPLFTYTEIDETDEA"
BASE COUNT          674 a          856 c          861 g          614 t
ORIGIN      
        1 aagggttaaa attttacgcg ggggtgatga cctatgagcg gtgaccaccg gggagggatc
       61 gcggtgaacg cgggggacgt caaaaaagcg gtaaaagccc gggaacggga gatcgtcagt
      121 ttcctttcca aggtccttca gactccaagc gtgacgggct acgaagccgc ggtggcggaa
      181 gtgatcgccc gggagatcga agggataggt ctcaggccgc ggatcgaaga agccgaaaaa
      241 ggaaggccca atcttttggc ggaatgggag ggcctgccgg ggccgcggtt tgtctttaac
      301 ggccacatgg atgtattccc cccgccggca ggttcagaag aggaatacgg cggaccctgg
      361 tcggggcggg tggaaggcgg ctggatcttc ggaagcggtg ccaacgacat gaaagcaggc
      421 ctctgtggct cgatcatggc cgtccgagta ctaaaggaaa tgggattcgt gccgaagggg
      481 agcgtcctgc tgacctgtac ctgcgatgaa gagaacggcg gctggctcgg agtgaaacac
      541 ctcttgggaa aagggttgat aaagggcgat ctcgggctga acatggaacc aacatcgctg
      601 aaacttctcc ttgaccacag cggcattttc agggcacgtt tcatcattga gggaaagggt
      661 gctcacgcga gcaaccccag cgaagggatc gacgccctgg aaaaatctat cggtgtcatc
      721 caggggcttt acgaactggg taagcggctg gaaaaacgat ccatccccgg ctggggaagc
      781 cctaccttga acgtgaccac cctccatgcg gggacagcga caaatgtcat tgccgaccga
      841 agcacctttt ccattgaccg aaggctggtc cccggggaaa gccatgatga ggcggaagcg
      901 gaaataatgg ctgtccttga agagcagagg aaaaaagacc cggacctgcg cctcgtccgt
      961 gagatcatct ccgaccggcc ggcgctggag atcccgcccg attcggccat cgtgaaagcg
     1021 atagagaagg gtttcaagga cctccagggc aagcctctgg aaagaatgaa ggcgcgcttc
     1081 ggctccgatg ccgccgacat tgtgaaagct accggcatgc caatgccaat cctcggtccg
     1141 gggaatgagg atgaagccac atcggttggc gaaaaattaa gtatcgaaga atacctagcc
     1201 gctgtcgaga tttacgccct taccgttgcc aggttgttat ctaccagggc ggggtcagac
     1261 ccacacggat cttcgccccg ggcctcgtct accacttaag ccaccgccct ggaagggcag
     1321 gctccattcc agcttgcgcc agcggacgac ataaaaaatg acggaggcgg catttcagag
     1381 ccaagtaaga ccggataacc ggccaacgga ccccaggagc cactcctccg tcaacaacat
     1441 agtaaacaaa ctcatggggc ctgtctaact caagaattca agggtaggcc ccgctgggct
     1501 taaaagcctt cgagctccag agtgtccagc gtcgtcacgg cgccttnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnctct cggaggagat cagcaggtcg tgattccccg
     1681 gcacgtagac caccttgatc gttccgtccc ggataatgtc gttgaacgcg tcgaccacgt
     1741 ccttgttgtt cgcggcgacg cgctgcacga actccttctg ggtctttccg gcgtacgtgt
     1801 ccgtgccggc ggggatgaac cactcgtcga cgaggtcgcc cgctacgacc agttccttga
     1861 tgttcggagc gcaccggacc ttccgaagaa agtcggcaag cggagcgcgg ttacgctgaa
     1921 tctccgcgta ccggtcgtcc atccccagat gtatgtcgct gatgacgacg atgacggagc
     1981 gttcgccgcc gagcgctgga acgccgctct catcgagagt gaaggcatag tccgacgcga
     2041 agcagagggg cgcagtgcac aacagtaaaa acaacagaat gatgctcttg atacccgttc
     2101 ttctcatggt ctcattccct ttcgtgtgcg aagtgatcgg ttgatgcgcg caactccgct
     2161 ttatcgtaat aaagttaagt ggcggtatgg gctgctctgt gcgaacaata ccacgaattt
     2221 ctcccttata ggtgtcagac ctacacaatt gatgaaacgc ccctctgccc ttcagagggg
     2281 cgtgacccga cctcggctga tcactgccag gggatcccaa gttccccagg gccgccttca
     2341 ggcctcgtcg gtctcgtcga tctcggtata ggtgaaaagg ggaccctcca ttttttccag
     2401 ttccctcagc gactcctcgt cgcccttctg ccttagcctc ccggcgcgca tccagtcgtc
     2461 ggactccagg gtggcaggga tcttcatccc gtcgatctcc gccatgatag cgacctcccg
     2521 gcagtagagc ttgcccccct cccggatgat ctcccacagg gggtattctt tgccccccac
     2581 ctcgatgagc tcatcgcttt tctttatgac ttccctcaag gctgggcgcc tcccccgcgt
     2641 ccggccttgc cctctctttt tcgccgagtc acggaacgag accgatccct taataagaac
     2701 cgtctacgga gcctctctcc ccttgagacg gcgctggtcc gagttcgtgg tttcgcccaa
     2761 aacgatttct tgctgattat cccacgatca ttacgtactc atccatttta gtaactgact
     2821 acaaagggac cgacaagcaa taaatttact caattggcgc ccaaattggg agcaagccgg
     2881 gcatcacggg ttgccttttc acctcttccg aagccggatg atcaaaagcg aagacagaac
     2941 ctcatctctt cattgcttta tcgggaatgt tatgctatct cttaacatat agtttcgagg
     3001 aaggaggggt gcttttgttc gaaaggctga gaagcgaggg caggataggt cgcttgacca
     3061 tccccaaccg cgtggtaatg cccgcgatgg ggacaaacct gtcgg
//
LOCUS       DAOSRG010000134         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28917_length_3061_cov_10.221224, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000134 DAOSRG010000000
VERSION     DAOSRG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28917_length_3061_cov_10.221224"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1143
                     /locus_tag="PLM30_05110"
     CDS             100..1143
                     /locus_tag="PLM30_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOR54583.1"
                     /translation="MHMADALLSPAVGGTMWAVTAGVTAYSAKKLKESGDDPKVPLMG
                     VLGAFIFAAQMINISIPATGSSGHIGGGMILAILLGPYAAFIVLASVLSVQALFFADG
                     GLLALGCNIFNLAFFPCFLAYPLIYRPIAGRNPSNLKILVGSVVSVVAALQLGAFSVV
                     LETLASGITDLPFKTFLLLMQPIHLAIGLVEGVITAAVVSYVWKARPAVIEAVQGDAP
                     SGVVHLKKVIVFFVAAAVITGGILSWFASNFPDGLEWAIEKTSGREELEAPEGGIHSV
                     LALLQEKFSFLPDYGFREPEAAEEGEAEGAAQEQSVMDPGTSMSGLVGGAIVFLLSAG
                     VGLILRKAAAGSR"
     gene            1187..1999
                     /gene="cbiQ"
                     /locus_tag="PLM30_05115"
     CDS             1187..1999
                     /gene="cbiQ"
                     /locus_tag="PLM30_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOR54584.1"
                     /translation="MSGSFGRGIGSVNSTRDFALLRSPVHSVDARSKWVVTLAFAVTV
                     TSFGKYDISRVMPLFLYPFVITALSGLPVPAVAKRAAAALPFILLVGVFNPLIDREPM
                     AGLGPVVLSGGWVSFISILVRSTLIVSSVVLLAMTTQPDNLFAALGSLGVPSALVVQL
                     QFMNRYIGLLAGEVERTLRAHSLRSHGKGGGVPVLVSGPMLGGLLLRTMDRGSRIHRA
                     MVSRGFDGAVRTARPSPFSPRRDGLFICAWLAYFALARFVDLPGVLLGVIGG"
     gene            1996..2772
                     /locus_tag="PLM30_05120"
     CDS             1996..2772
                     /locus_tag="PLM30_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOR54585.1"
                     /translation="MRGPYIDMKGLEYSYPDGTPALRGVSFVADRGEAVGLVGANGAG
                     KSTLLLALTGLIEPSSGVIDIGGTVLSKRTAPEVRRRLGFIFQDPEDQLFMPTVFDDV
                     AFGPINQGVTGEALAARVASALESVGAGHLADRPGYRLSGGEKRSAAIAAVLSMGPEI
                     LLMDEPSSGLDPKGRRGLIDLLAGFPHTRLIAAHDLDLVLDVCLRTIVLHRGEVAADG
                     PTLEIFRDRDLLERCSLEPPLSLQGLFGGRTAADDVTLGR"
     gene            complement(2804..>3061)
                     /locus_tag="PLM30_05125"
     CDS             complement(2804..>3061)
                     /locus_tag="PLM30_05125"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HOR54586.1"
                     /translation="NFEEVLIPWDLTRAQIPDPLNIFENSAVGSGGELVHLPVVSKAG
                     DHITFRALMNLVCAVSACPMDLNITGGERITDMLVTIVRGD"
BASE COUNT          449 a          978 c         1016 g          618 t
ORIGIN      
        1 ctgggtccaa agtggattgg gcctcccatt ttccctgttg acaggggagc cccgaagtat
       61 tacaaatatc gaaatacgta ataaaaggag gctccccaca tgcacatggc cgatgctctt
      121 ttgtctcccg cggtgggggg gactatgtgg gcggtgaccg ccggggtcac agcctactcg
      181 gcaaagaagc tcaaggagtc cggcgatgac ccgaaggtcc ccctgatggg cgtgctcggc
      241 gcctttatct tcgcggccca gatgatcaac atcagcatcc cggcaacggg ttccagcgga
      301 catataggag gggggatgat cctggccata ttgctggggc cttacgcggc cttcatcgtc
      361 cttgcctcgg tcctttcggt ccaggccctg ttcttcgccg acgggggcct tctcgccctg
      421 gggtgcaata ttttcaacct cgcctttttc ccctgcttcc tggcgtaccc cttgatctac
      481 cggcctatag cgggcaggaa cccctcgaac ctaaagatcc tggtagggtc ggtggtttcc
      541 gtcgtggcgg ccctccagct gggggccttc tcggtcgtgc tggagacgct ggcctcgggc
      601 attacggacc tgcccttcaa gaccttcctc ctcctgatgc agcccatcca cctcgccatc
      661 ggcctcgtgg aaggggtcat caccgcggcc gtcgtgtcct acgtctggaa ggcgcgcccc
      721 gcggtgatcg aggcggtgca gggcgatgcc ccttccgggg ttgtacacct caagaaggtc
      781 atcgtatttt tcgtcgccgc ggcggtgatc acggggggga tcctttcctg gttcgcctcg
      841 aacttccccg acggtctcga gtgggccata gagaagacct cgggccgtga ggagctggag
      901 gcccccgagg gcggtatcca ttcggtcctg gcattgttgc aggaaaagtt ctccttcctt
      961 ccggactacg ggttccgtga acccgaggcc gccgaggagg gtgaagccga aggcgccgcc
     1021 caggaacagt ccgtcatgga ccccgggacc tccatgtccg gcctcgtcgg tggggcgatc
     1081 gttttcctct tgtccgccgg ggtggggttg atcctgagaa aggccgcggc cgggagccgc
     1141 tgacggcctt tgcgccgctt cccggtcgca gggtaaggag ccctttttgt ccggcagttt
     1201 tggcaggggg atagggagcg tcaattccac ccgggatttc gcccttttac ggagcccggt
     1261 ccattccgtc gacgccaggt cgaaatgggt cgtgaccctg gcctttgccg tcacggtcac
     1321 atccttcggg aagtacgata tttcccgggt gatgccgctt ttcctttacc ccttcgtcat
     1381 aaccgccctt tcgggcctgc ccgtcccagc cgtggccaag agggcggcgg cggcgctccc
     1441 cttcatcctg ctggtgggcg tcttcaaccc cctcatcgac agggagccca tggccggact
     1501 gggcccggtg gtcttgagcg gaggatgggt ctccttcatt tccatcctgg ttcggtcgac
     1561 cctcatcgta tcgtcggtgg tgctgctggc gatgaccacg cagcccgaca atctcttcgc
     1621 ggccctgggg agtctgggcg tcccctcggc gctcgtggtg cagctccagt tcatgaaccg
     1681 ctatatcggc cttttggcgg gcgaggtgga gaggaccctc agggcccatt cgctgaggtc
     1741 ccacggcaaa gggggcgggg tcccggtgct ggtgtcgggc cccatgctgg gtggcctcct
     1801 gctgaggacc atggacaggg gctccaggat ccaccgggcc atggtaagcc ggggtttcga
     1861 tggggccgtc aggacggccc gcccctcgcc tttctccccc cggcgggacg gcctgttcat
     1921 ctgtgcatgg ctggcctatt tcgccctggc gcgtttcgtg gacctgccgg gcgtccttct
     1981 cggggtgatc gggggatgag gggcccttac atcgacatga aggggcttga gtattcgtac
     2041 cccgacggga ccccggcgct gaggggggtt tccttcgttg cggatcgggg cgaggccgtg
     2101 ggcctcgtcg gggccaacgg cgccggcaag tcgacactcc tgctggccct gacgggcctg
     2161 atagagcctt cgtcgggggt catcgatata ggcggtacgg tcctttccaa gaggacggcc
     2221 ccggaggtgc gccgacgcct ggggttcatc ttccaggacc ccgaggacca gctcttcatg
     2281 cccaccgttt tcgacgacgt cgccttcgga cccatcaacc agggggtgac cggtgaggcc
     2341 ctggccgcca gggtcgcctc ggcccttgag agcgtcggcg cggggcacct cgccgaccga
     2401 ccgggctacc gcctctccgg gggggagaag aggtccgccg ccatagccgc cgtcctctcc
     2461 atgggcccgg agatcctcct catggacgaa cccagttcgg gcctcgaccc gaagggccgg
     2521 agggggctga tagacctcct ggcgggcttt ccccacacgc gcctcatcgc cgcccacgac
     2581 ctggacctcg tcctggacgt ctgcctgcgg acgatagtgc tgcaccgggg agaggtggcc
     2641 gccgacgggc ctacgcttga gatcttccgg gacagggatc tgctggaaag atgctccctg
     2701 gagcccccgc tgtcgctgca gggcctcttc ggcgggcgca ccgcggcgga tgacgtgacc
     2761 ctggggcggt gaaggaacgg gacctttcgg tgggagctcc cggtcaatct ccccgtacga
     2821 tggtcaccag catgtcggtg atcctctcgc cgccggtgat gttcaggtcc atggggcagg
     2881 ccgagacggc gcagaccagg ttcatcaggg ccctgaaggt gatgtggtca ccggccttcg
     2941 agaccaccgg caggtggacc agttcaccgc cggagccgac ggcggagttc tcgaagatgt
     3001 tgagggggtc gggtatctgc gcccttgtta aatcccaggg gatgaggacc tcctcgaagt
     3061 t
//
LOCUS       DAOSRG010000135         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_28945_length_3059_cov_7.613515, whole genome shotgun sequence.
ACCESSION   DAOSRG010000135 DAOSRG010000000
VERSION     DAOSRG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28945_length_3059_cov_7.613515"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="aspS"
                     /locus_tag="PLM30_05130"
     CDS             <1..1421
                     /gene="aspS"
                     /locus_tag="PLM30_05130"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR54587.1"
                     /translation="RSRYLDLRRGSMQDNLRLRSRAAEFTRSFLAGEGFIEVETPMLT
                     KSTPEGARDYLVPARVTPGRFFALPQSPQIFKQILMVGGIDRYYQIVKCFRDEDLRAD
                     RQPEFTQIDVEMGFITEEDIMALMEGLMKGLFQSVLGDCLEVPFQVLSWHDAIDRFGS
                     DRPDLRIEGELQDLDECFRGCTFEVFLKTLEGGGVARGLRVPGGASLSRKEMSDLEAR
                     SRELGASGLAFFSLREAVLKGPLVKHLSNGAQALLVERTGLKEGDAIAVAAGPWKKTC
                     EVLGQLRLEIGARFFPRREGAWHFLWVTDFPLLEWDDEELRWTAVHHPFTSPNLQDLE
                     KLESDPGQVRSRAYDLVLNGTEIGGGSIRIHDPSLQRRVFEVMGFTPERARERFGFLI
                     DALASGTPPHGGIAIGFDRLVMFLAGVHSIRDVIAFPKTQKAQCLMSGAPNIVDPGQL
                     AELHISLVPNGKDPGCSSGDGE"
     assembly_gap    1552..1651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1652..2296
                     /locus_tag="PLM30_05135"
     CDS             <1652..2296
                     /locus_tag="PLM30_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOR54588.1"
                     /translation="VEGGGLDGLIDPFLTGNSMTPFSPEDFKDVNFIFVTHGHGDHLG
                     DTPAIAKRTGATVVATVEMCDVLKGVKCHPLQIGGAFTFPFGRVKMTPAWHGSGVQGG
                     EGEGMAYGGVAGGFLLEVDGRRIYHAGDTGLTVEMGLLAEDGVDVALLPIGGNYTMDI
                     DDAVRAVRMIKPRVAVPMHYNTFPLIKASPEEFASKASGLAEIRILKPGEILEV"
     gene            complement(2313..>3059)
                     /locus_tag="PLM30_05140"
     CDS             complement(2313..>3059)
                     /locus_tag="PLM30_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOR54589.1"
                     /translation="DVADGLGVSKAQMVRVSRLLGFEGYADLKETLQDAVLGQVNPAA
                     MLARVMDNRQDLPVTIHRLEHANLDDTWSQLRSEKVSAFCSKLRDADAVYCAGWGIST
                     LVAQSLFMRLRIMGMKAFLMTRSAMTLQEQARSVGPGDVVVVCELPSFVIEVTQAMER
                     AKTKGAGIITITDNPAAPICRYSDLSFFVSAASPMFGSSVIGPLFLVHVLTSVLAVNM
                     GDSARKALEEQSEFLHDERIFHPVFGLKYS"
BASE COUNT          565 a          906 c          955 g          533 t
ORIGIN      
        1 tgaggtcgag gtacctcgac ctccggaggg gctccatgca ggacaacctg aggttgagga
       61 gcagggccgc ggagttcact cggtccttcc tcgccggcga gggattcatc gaggtcgaga
      121 cgcccatgct gaccaagtcg acccccgagg gggccaggga ctacctcgtc ccagcgaggg
      181 tgacgcccgg ccgtttcttc gcccttcccc agtcaccaca gatcttcaag cagatcctca
      241 tggtgggcgg catagatcgc tattaccaga tagtcaagtg cttcagggat gaagacctcc
      301 gggctgaccg gcagcccgag ttcacccaga tcgacgtcga gatgggtttc atcaccgagg
      361 aggacatcat ggctctcatg gaagggctga tgaaggggct tttccagagt gtcctgggcg
      421 attgcctgga ggtccccttc caggtgctgt cctggcatga cgccatcgac cgcttcggca
      481 gcgaccggcc cgacctgagg atcgaggggg aactgcaaga cctggacgag tgcttccggg
      541 gatgcacttt cgaagtcttc ctaaaaaccc tcgaaggggg aggagtcgcg agggggcttc
      601 gcgtacccgg cggggcctca ctctcgcgta aggagatgtc ggacctcgag gccaggtcca
      661 gggaactggg ggcctcaggt ctagccttct tttcactccg cgaggcggtc ctcaagggtc
      721 ccctggtcaa gcacctttcc aacggggccc aggcactcct cgttgaaaga accggcctca
      781 aggaagggga cgccatcgcc gtcgccgcgg gaccctggaa gaagacctgc gaagtgctgg
      841 gccaactcag gctcgagata ggggcaaggt tcttccccag gagggaaggg gcctggcact
      901 tcctttgggt caccgatttc cccctgctgg agtgggacga cgaggaactc cgctggacgg
      961 cggtccacca ccccttcacc tcgccgaacc tgcaggacct ggaaaaactc gagtcggacc
     1021 ccggccaggt caggtccagg gcttacgacc tggtcctgaa cggtaccgag ataggggggg
     1081 gctccatcag gatccatgac ccctcgctcc agcggagggt cttcgaggtg atgggcttca
     1141 ccccggaacg ggccagggag aggttcggct tcctgatcga tgccctcgcc tcaggtactc
     1201 ctccccatgg agggatcgcc atcggtttcg acaggctggt catgttcctg gcgggcgttc
     1261 attcaatacg ggatgtgatc gctttcccga agacccagaa ggcccaatgc ctcatgtcgg
     1321 gcgctcccaa catcgtcgac cccgggcagt tggccgaact ccatatttcc ctggtcccga
     1381 atgggaagga cccaggatgc tcctccgggg acggggagtg acgaccgggg ggaagaagaa
     1441 gccctggcgc ttcctcctgg aagagcggca gggccggttg gcgggcgaac tgcaagccga
     1501 cggctggagc ggttcattca agatgaacgc ctggttcgaa aagcatgccg gnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgagggc ggcggcctcg atggcctgat
     1681 cgaccctttc ctcacgggga acagcatgac ccctttctcc ccggaagact tcaaggatgt
     1741 caacttcatt ttcgtgactc acgggcacgg tgatcatctc ggggacaccc cggccatcgc
     1801 caagaggacc ggggccactg tggtcgccac agtggagatg tgcgacgtgc tgaaaggggt
     1861 caagtgccac ccgttgcaaa taggcggggc tttcaccttc cccttcggaa gggtgaagat
     1921 gacgccggcc tggcacggtt cgggcgtcca gggcggcgag ggtgagggta tggcctacgg
     1981 gggcgtggcg ggagggttcc tcttagaagt cgacggccgc aggatctacc atgccggcga
     2041 cacggggctc accgtcgaga tggggctcct cgccgaggat ggggtggatg tggccctact
     2101 ccccataggc gggaattaca ccatggatat cgacgacgcc gtcagggctg tcaggatgat
     2161 aaaaccccgg gtggccgtcc cgatgcatta caacactttc ccgctgataa aggcctcccc
     2221 cgaggagttc gcctccaagg cttcggggct cgcggagata aggatcctga aacccgggga
     2281 gatcctggaa gtctgaccgc cgccgcttta gctcaggagt acttcagccc gaaaacgggg
     2341 tggaagatcc tctcgtcgtg aaggaactcc gactgctcct ccagggcttt cctcgccgag
     2401 tcccccatgt tgacggcgag gaccgaggta aggacgtgca ccaggaacag ggggccgatg
     2461 acgctgctcc cgaacattgg gctcgcggcg ctcacgaaga aggaaaggtc cgagtaccgg
     2521 cagatcgggg ccgcggggtt gtcggtgatg gtgatgatcc cggccccctt cgttttggcc
     2581 ctctccatcg cctgggtgac ctcgatcaca aaactcggga gttcacacac gacgaccacg
     2641 tccccgggcc cgacgctcct ggcctgttcc tgcagggtca tggcggaacg ggtcatcagg
     2701 aaggccttca ttcccatgat ccttagcctc atgaacagag attgggccac cagggtcgag
     2761 atcccccacc cggcgcagta gacggcatcg gcatcgcgga gcttcgagca aaaggcggag
     2821 accttttcag acctcaactg ggaccatgtg tcatccaggt tcgcgtgctc cagcctgtgg
     2881 atcgtgacgg ggaggtcctg ccggttgtcc atcacccggg cgagcatcgc ggccgggttg
     2941 acctgtccca gcacggcatc ctggagggtt tccttcaggt cagcgtaacc ttcaaaaccg
     3001 agcagcctcg agaccctcac catctgggcc ttggacaccc cgaggccatc ggccacatc
//
LOCUS       DAOSRG010000136         3037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29230_length_3037_cov_10.576794, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000136 DAOSRG010000000
VERSION     DAOSRG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29230_length_3037_cov_10.576794"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLM30_05145"
     CDS             complement(<1..1061)
                     /locus_tag="PLM30_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOR54590.1"
                     /translation="MLSLGFGAGFPGIDAVEALREMEGRLKKGSQRLLEGARAKEDGF
                     GWLDLPDSPPEGLFDSVAWLSGFDSVVQIGIGGSALGNRMISSALGHPFYNELTRKER
                     MAPRFYIADNADPEKASAILGMIDPSRSGFIVASKSGSTAETMANFLFFLGELERSGV
                     KDPAGNILVVTDEKRGVLRSFAKESGCRSLPVPEAVGGRYSVLSAVGLLSAGAQGIDI
                     SSLLLGASVMKKNLSGRMGTGEDPARVLAASAIRHNEEGRNILVLMPYSDNLASFSEW
                     FCQLWAESLGKEGWGSTPLRAQGATDQHSLLQLFTDGPDDKLYLFLVPASRRDLVLPG
                     ASWESLEELSYLGDKTMGS"
     gene            complement(1191..1433)
                     /locus_tag="PLM30_05150"
     CDS             complement(1191..1433)
                     /locus_tag="PLM30_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOR54591.1"
                     /translation="MTKVKVYSTPTCPWCVRLKNYLQSKKVDFENIDVSADRAAAMEM
                     VKKTGQMGVPVALIGDVYIVGFDQARIDEELAKLGG"
     gene            1731..2087
                     /locus_tag="PLM30_05155"
     CDS             1731..2087
                     /locus_tag="PLM30_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOR54592.1"
                     /translation="MILGDVMKILEAEPLSRALNLDVDLHTAFAADLMSDVLALARPG
                     TLMITGLTNIQIMRTASVVDTPAVVFVRGKKPPKETIDLAEKLGIPSIITSKTMFEAC
                     GLLFQAGIKPIRCVER"
     gene            2093..2563
                     /locus_tag="PLM30_05160"
     CDS             2093..2563
                     /locus_tag="PLM30_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54593.1"
                     /translation="MSRQEPYVIQREVKPLDFLAAGETASDFKETLKSLGIPPEICRK
                     ASIVTYEAEMNAVIHGGGGVIALELAGDRITIVASDEGPGIENVSLAMQEGYTTAPDS
                     IRELGFGAGMGLPNIKKYSDELEIETELGKGTTLRAVILLGPSGEEVSDLCRTA"
     gene            2548..>3037
                     /locus_tag="PLM30_05165"
     CDS             2548..>3037
                     /locus_tag="PLM30_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOR54594.1"
                     /translation="MPHGIKISGESCRGCVNCIKACPTEAMRVIHGQLHIIDERCIAC
                     GECLRICRHRAISLEESEWNSIAADNRSMPLVTDPALCTQFSWPPDPGILKDILMAVG
                     FEPIFEDTALAFDLAAMAQARILDEHDSRELPMISTYCPSVVRLIQIRFPELIHNLSP
                     AET"
BASE COUNT          683 a          904 c          809 g          641 t
ORIGIN      
        1 atggagccca tggtcttgtc accgaggtaa gagagctcct ccaggctttc ccaggatgcc
       61 cctggcagga ccaggtccct cctggaagcg ggaacaagga agaggtacaa cttgtcgtcg
      121 ggtccatcgg tgaaaagctg cagcagcgag tgctggtccg tcgcgccctg ggccctgaga
      181 ggagtgctgc cccacccctc ctttcccagg ctctcggccc agagttggca gaaccactcc
      241 gagaaagaag ccaggttgtc cgaatagggc atgaggacga gaatgttcct accctcctcg
      301 ttgtggcgta tcgccgaagc cgccaatacc cttgccgggt cctcgcctgt ccccatcctg
      361 cccgacaggt ttttcttcat caccgaggcc ccaaggagga gggaactgat atcgatcccc
      421 tgagcaccgg ccgagagaag ccccacggcg gaaagaaccg agtacctgcc tcccacggct
      481 tcaggcacgg ggagcgaccg gcacccggac tccttcgcga aggaccggag gacgcctctt
      541 ttctcgtcgg tcacaacgag gatgttcccg gcaggatcct tgacgccgga cctttcgagc
      601 tcgccaagga aaaagaggaa attggccatg gtctcggccg tcgaacctga cttgctggcg
      661 acgataaaac ctgaacgtga tgggtcgatc atgcccagga tggcggaagc tttttcaggg
      721 tcggcgttgt cggctatgta aaacctcggt gccatcctct cctttcttgt cagttcgttg
      781 tagaaagggt gaccgagggc cgaggagatc atcctgttcc cgagggcgga cccgcctatc
      841 ccgatctgga ccaccgaatc gaagccgctg agccaggcca ccgaatcaaa caggccttcc
      901 ggggggctgt cgggcaggtc aagccatcca aaaccatctt ctttcgcccg cgctccctca
      961 agaagccttt gcgacccttt cttcaaccgc ccttccatct cgcggagagc ctccaccgca
     1021 tcgatgccgg ggaaaccagc gccgaaaccc aggctaagca tgttctccct caaagtgatc
     1081 accgcctcgg caagtgggcc ttttttagat agagtggtct tattttaaca gaaaaaaaag
     1141 gctgcctcct cgataggggc agcccagaat gatctatttc gtctaccggc ctaaccgccg
     1201 agtttggcca attcctcgtc tatcctggcc tggtcaaaac ccacgatata gacatcgccg
     1261 ataagggcca cgggtacccc catctggccg gtcttcttaa ccatctccat cgcggcggcc
     1321 ctgtcggcac tgacatcaat attctcaaaa tcaacttttt ttgattgaag gtaattcttg
     1381 agcctgacgc accacggaca ggttggtgtc gaataaactt taacctttgt cactaccttt
     1441 cacccccaac ttttttaccc taagggatta actgaatacc cccagtaggt attttacact
     1501 cgggcctcgc tttgtcaacc ttgactttaa cacctttgag gagcccttgg aagagaacct
     1561 ataaagagta agcccaaagg tactatcctg tgtaaaaagg cccctatctc ggccaggccc
     1621 tctctggacc gcgaaacggt aaatttttca ctaacaggaa gagcatatca aaaagaaaca
     1681 tgctatagtt agcagggcca ctttttccat tatattaagg agggggacaa atgatcctgg
     1741 gcgacgtcat gaagatcctt gaagcggaac cgcttagccg ggcattaaat ctcgacgttg
     1801 accttcatac ggcttttgcc gccgatctca tgagcgatgt cctcgcgctg gcgagaccgg
     1861 gcactctcat gatcaccggg ttgaccaata tccagatcat gaggacagcg agcgtggttg
     1921 acacgccggc tgtcgtattc gtccgcggga aaaaaccgcc caaggagacg atcgatcttg
     1981 cggaaaagct cggcatcccc tccatcatta catccaagac aatgttcgag gcctgtggcc
     2041 tgctattcca ggcagggatc aagcccatcc gttgcgtcga aaggtgaacc ccttgagccg
     2101 acaggaacct tacgtcatcc aaagggaggt caaacccctg gattttcttg ctgctgggga
     2161 gacggcgtcg gatttcaagg agaccttgaa atccctcggc atcccccccg agatttgccg
     2221 gaaagcctcc atcgtgacct atgaagcgga gatgaacgcc gtgatccatg ggggcggcgg
     2281 cgtaatagcc ctggaattgg ccggcgaccg gatcaccatc gtggccagtg acgagggtcc
     2341 gggcattgag aacgtaagcc tggcgatgca ggagggatat accacggccc cagattccat
     2401 cagggaattg gggttcggcg ctgggatggg cctccccaat atcaagaagt attccgacga
     2461 acttgagatc gaaaccgaat tggggaaggg gacgaccctc agggccgtca tccttttagg
     2521 cccttccggg gaggaggtga gcgacctttg ccgcacggca taaagatctc cggagaatcc
     2581 tgccgggggt gcgtcaattg catcaaggct tgtcctactg aggccatgag ggtgatccac
     2641 ggtcagttgc acatcatcga cgaacgctgt atcgcctgtg gcgaatgcct caggatatgc
     2701 cgccacaggg ccatttcgct ggaggaatcc gaatggaatt ccatcgccgc cgataatcgt
     2761 tcgatgcccc ttgtgaccga ccccgcgctc tgcacccagt tctcctggcc cccggacccc
     2821 ggtatactca aagatatcct tatggctgtt gggttcgaac cgatcttcga agataccgct
     2881 ctcgccttcg acctcgccgc catggcccag gcccgtatcc ttgatgagca cgattcccgc
     2941 gaattaccga tgatatcgac ctattgccct tccgtggtga ggctgataca gatcaggttc
     3001 ccggagctga tacacaacct ttcgcccgcc gaaaccc
//
LOCUS       DAOSRG010000137         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29364_length_3027_cov_7.762786, whole genome shotgun sequence.
ACCESSION   DAOSRG010000137 DAOSRG010000000
VERSION     DAOSRG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29364_length_3027_cov_7.762786"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..961
                     /locus_tag="PLM30_05170"
     CDS             44..961
                     /locus_tag="PLM30_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOR54595.1"
                     /translation="MKKNFQKRYDMKRNEQPSLLDRIASVSSTLTGQQMALARFIYQD
                     PEKSAFLNSVQLSEAAKVSTPTVVRLAVKLGFKGFPDFREALRDTVRSRIRSIDRYRE
                     EPGLEEEPLFQRVLSLEFHVLDEMKRHLREESVQKAVDLLSRKRRIFIAGFLANICHA
                     EYMAYFLGILREGVHLLTRAGENLFAQLQKAGPEDGAIIYSFPRYPTATQKLARYLKD
                     KGVPIIGVTDSALSPLAAFSDILLEAPMKFISFIDPCAGAFALNHCLLTAFYFRYPEK
                     MRETLLEFEKYSRSQNLFLMEDIDIVDLV"
     gene            987..1970
                     /locus_tag="PLM30_05175"
     CDS             987..1970
                     /locus_tag="PLM30_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54596.1"
                     /translation="MEVRLLSRKEVESLQIPVTEVMDVVEKGFALKGKDAMEMPAKIG
                     IHPRKDCFIHAMPCFVGGGADVAGIKWVSGYPLNQAKGLPYITGVMCLNDPETGFVKV
                     IMDANWITAWRTGAATGVCARHMADPDLRVLAVIGLGVQGRTNAAALAAALPKVEKVK
                     VYDNHAEQVPRFLELVKEDLKGIEVLPCATVEETVREADVVVTCTPIVNDPERFVRSK
                     WLKKDMLAVAVDYDSAFEADVMSGASAFVCDDLNQYLWTQEQGHYFQKGYPTEEQILG
                     DMGHLCAGLRKASTKGRRGAVLMGIASHDVLTANLIHEKALARGLGTVVDI"
     gene            complement(2027..2752)
                     /locus_tag="PLM30_05180"
     CDS             complement(2027..2752)
                     /locus_tag="PLM30_05180"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOR54597.1"
                     /translation="MDTKKMARRIREDLLSLGDEALRSSQARFFREEIKCHGIRSALV
                     RQYGRMIAKEASGQSKEKVFELCELLWRGGYLEETFIACHLTESRAKEFDKADIGLFQ
                     HWVGSWVTNWASCDTFCNHSVAMLLDKYPENIGVLKKEWATSPNRWLRRGAAVSLVIP
                     ARRGDYLEEVMEIASILLLDDDDLVQKGYGWMLKAASESHRQEIFEFMMGRKDTMPRT
                     ALRYGIEKMPSEMRKMVMDRGRG"
BASE COUNT          641 a          882 c          858 g          646 t
ORIGIN      
        1 cgtgaggaat agatcattga cagggggagg actgtcgtga ataatgaaga aaaattttca
       61 aaaaaggtat gacatgaaaa ggaatgaaca accatctctg ctggacagga tcgcctccgt
      121 ctccagtacc ttgacggggc agcagatggc cctggcccgt tttatttacc aggaccccga
      181 gaagtcggcc ttcctgaact ccgttcagct gtcggaagcg gcgaaggtca gcacccccac
      241 cgtcgtcagg ctcgcggtga aactgggttt caagggtttt cccgactttc gggaggccct
      301 ccgggacacg gtccggtccc gcatccgctc catcgatcgt tacagggagg aaccggggct
      361 tgaggaggaa ccccttttcc aaagggtcct ttccctcgag ttccatgttc tggacgagat
      421 gaaaaggcat ttgcgcgagg agagcgtgca aaaggcggtg gatcttctct cgagaaaacg
      481 caggatcttc atcgcgggtt tcctggcgaa tatctgccac gccgagtaca tggcctattt
      541 cctgggtatc ctcagagaag gggtccacct cctgacccgg gccggggaaa accttttcgc
      601 gcagcttcag aaggcgggac ccgaggacgg ggcgatcatc tacagctttc cccgttaccc
      661 cacggcaacc cagaaacttg cccgttacct caaggacaag ggtgtcccca tcatcggtgt
      721 gaccgattcg gccctctctc ccttggccgc cttttcggac atactgcttg aagcgcccat
      781 gaagttcatt tctttcatcg acccctgcgc aggggctttc gcgctcaacc actgtctgct
      841 gacggctttt tacttcaggt acccggaaaa gatgcgggag accctgttag agttcgaaaa
      901 gtattctcga agccagaacc ttttcctgat ggaagatatc gacatcgtcg atctcgtcta
      961 aatcaaccgc aaaaggagtg gttcccatgg aagtaaggct gctgtccagg aaagaggtcg
     1021 agtcgctgca gatcccggtg acagaggtca tggacgtggt cgaaaagggc ttcgccctga
     1081 agggaaagga tgccatggag atgcccgcca agatcggcat ccacccgagg aaggactgct
     1141 tcatccacgc catgccctgc ttcgtgggcg gaggagccga tgtggccgga ataaagtggg
     1201 tctcggggta tccgctcaac caggcgaagg gtttgcccta catcaccggg gtcatgtgcc
     1261 tcaacgaccc ggagaccggt ttcgtgaagg tcatcatgga tgccaactgg atcaccgcct
     1321 ggaggaccgg ggccgcgacg ggcgtatgcg caaggcacat ggccgacccc gatttaaggg
     1381 tcctggccgt aatagggctc ggggtccagg gtaggacgaa tgcggcggcc ttggccgcgg
     1441 ccttgcccaa ggtggagaag gtcaaggtat acgacaacca cgccgagcag gtcccacggt
     1501 tcctggaact ggtgaaggaa gacttgaagg gtatcgaggt cctcccctgt gccaccgtcg
     1561 aggaaacggt gagggaggcc gacgtggtgg tcacctgtac ccctatagtc aacgaccccg
     1621 agaggttcgt ccggtccaag tggcttaaga aggatatgct ggccgtcgcg gtagactatg
     1681 attccgcctt tgaagccgat gtgatgtcag gggcttccgc cttcgtgtgc gatgacctga
     1741 accagtacct ctggacccag gaacagggcc attactttca aaaggggtat cccacggaag
     1801 aacagatact gggcgacatg gggcacctgt gcgccggcct gcggaaggcc tcaacgaagg
     1861 ggcgccgtgg cgccgtcctg atggggatcg ccagtcacga cgtcctgacg gcgaacctga
     1921 tccatgaaaa agcccttgcc aggggccttg gcacagtagt ggatatctag ccctcgagga
     1981 tgaagcgggg ggtggggagg atggccgccc cccgctcaag gaagttctac cctctacccc
     2041 ggtccatgac catttttcgc atctccgacg gcatcttttc gatgccgtat ctcaaggcgg
     2101 tcctgggcat ggtatccttc ctgcccatca tgaattcgaa gatctcctgt cggtgggatt
     2161 cactggcggc cttaagcatc cacccgtaac ccttctggac caggtcatcg tcgtcgagga
     2221 gcagtatact cgcgatctcc atcacttctt caaggtagtc cccacgacgg gcggggatga
     2281 ccagggagac agccgccccg cgcctgagcc atcggttcgg cgaggtcgcc cattcttttt
     2341 tgaggacgcc gatattttcg gggtacttgt ccagaagcat cgccacgctg tggttacaga
     2401 aggtgtcgca ggatgcccag ttcgtaaccc aggatccaac ccagtgctgg aataacccga
     2461 tatcggcttt atcgaattcc ttggccctgg attcggtcag gtgacaggca atgaaggtct
     2521 cctcaagata gccgcccctc caaaggagtt cgcagagctc gaagaccttc tccttgcttt
     2581 gccccgatgc ttccttggct atcatcctcc cgtattgcct gacaagggcc gatcttatgc
     2641 cgtggcattt gatctcttcc ctgaaaaacc tcgcctggga cgaccggagg gcttcgtcac
     2701 ccagggacag caggtcttcc ctgatcctgc gggccatctt cttcgtgtcc atagtatctt
     2761 cccccaacgg tcgaattctt taacgatccc gaggaccact ccttcgggca cgctcccgcc
     2821 cattatcccc tctacatact cgttttcgat aaacccccta gagctgaaaa ccccacgaac
     2881 tttcaccatc tccttcgccg cctggcttta taatgatcac gggtcatccc ggaaaggggg
     2941 gttatccctt gatagacctt cactacggga gacaagcctt cgatttcgat tgcggcgcca
     3001 cggccctgca aaccgttatg gcctact
//
LOCUS       DAOSRG010000138         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29600_length_3008_cov_10.429055, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000138 DAOSRG010000000
VERSION     DAOSRG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29600_length_3008_cov_10.429055"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2166
                     /locus_tag="PLM30_05185"
     CDS             <1..2166
                     /locus_tag="PLM30_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR54598.1"
                     /translation="DVLIVIFAGYPSEMDALFEAAPGLRSRVPFHLDFPDYSPAELLD
                     IMLFMAASEELELSDEAAGKFLRIMGERVGQPDFSNAREVRNLLDQAKGELSRRLQTR
                     RKVSRWDMKVITALDLGRGDPELLSSLETARKEMFRVPLDPGPRSAFASLCAMAGMWS
                     DAVSALEAVEKGLTPEYRALLGRGLYILGDRERSWEVFSAMGEPTAGSYYRGLSALWA
                     GDLSTASAMLEQASSDDPGSPDVLLALAAVKFFAGAFREAADSFLAGMFSSGGKLPPA
                     ALRDLPWEKLPWPGSARPLRDALFFAYGHPDRSKLLFTEALIATGTKEALDPAGEIVM
                     DSIDRIPDEPLAHRLMSVIHEASGRTLEAVASLDVALELEPRNTGDWRRLALLLEQVG
                     EKERAEGIFRDILEEDPTGEAGIRLAMAADDKGDQEEAEALYRKAWEAGLRGEERSLC
                     ARRLGSLSAASGRFDEALEFFAEVGGPAGDPRAAFWHARSLIEDERWQEAENLLVTLY
                     DDPDLDSPRVFWLGRLFLARKDLFSARNIKWDGLANPYMRLVKGVVEAFSGDDRATET
                     LSDLPAPGMGGDAKAMLCSARVALKDWEGAKRFGRMALEGGKGPCLWERQTRRPLEEA
                     GYMTAIAEAHLGDWAAARDGFRRSASSLRHPGPVFSLGVSLVALGKIDEARVILTQLK
                     PASAVLATKLEELIVQNVGIRKMLADPVDPVLLDLYASL"
     gene            2218..2799
                     /locus_tag="PLM30_05190"
     CDS             2218..2799
                     /locus_tag="PLM30_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR54599.1"
                     /translation="MKKVDREEAMALLREHNRQIGHIRHALAVEAAMRFFARANGEDE
                     EEWGLAGLVHDIDWETTIDDPSRHTHLGASWLRDAGYPDNIVRAVLAHGWGICSDVEP
                     LSPMEKHLYAIDELTGFVTAVALVRPGKSLQDLTVKSVRKKWKDKAFARGVNREVIEK
                     GTAMLEKPLEELVEGVIEALRPIEKDIGLGEED"
     gene            complement(2796..>3008)
                     /locus_tag="PLM30_05195"
     CDS             complement(2796..>3008)
                     /locus_tag="PLM30_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54600.1"
                     /translation="LLARDDLIPWEGIHGGTMKIYDPSLVWFAGPRWCVAWAEDGHIG
                     GFMLLRFDEDDDGNLEWRLLDSALAD"
BASE COUNT          572 a          873 c         1001 g          562 t
ORIGIN      
        1 gacgtcctga tcgtcatctt cgccggttac ccctcggaga tggacgcctt gttcgaggcg
       61 gcgccgggtc tcaggagcag ggtccccttt cacctggatt ttcccgatta ctccccggcg
      121 gaactgctgg acatcatgct tttcatggcc gcctcggagg aactggaact gtccgacgaa
      181 gccgccggga agttcctgcg gataatgggc gaacgggtag gccagcccga tttcagcaac
      241 gcccgggagg tcaggaacct ccttgaccag gccaagggcg aactctcccg gaggcttcag
      301 acccgacgga aggtttcacg gtgggacatg aaggtgatca ccgccctcga tcttggtcgg
      361 ggtgacccgg agttgctttc atccctcgag actgcccgta aggagatgtt cagggtcccc
      421 ctcgaccccg ggccgaggtc cgctttcgct tccctctgtg cgatggcggg gatgtggtcc
      481 gacgcggttt cggccctcga ggccgtggaa aagggcctca caccggaata cagggccctc
      541 ctggggagag gtctttacat cctgggcgac cgggaaaggt cctgggaggt cttctcggcg
      601 atgggcgaac ccaccgctgg atcctactac agggggctct cagccctatg ggcgggtgat
      661 ctttccacgg cgtccgccat gctcgaacag gcttcatcgg acgaccccgg ttcccccgat
      721 gttctgctgg cgctcgcggc cgtgaaattt ttcgccggcg ctttccggga ggcggccgat
      781 tccttcctgg cgggaatgtt ctcttccggc gggaaactgc cccctgccgc attaagggac
      841 ctaccctggg aaaaactccc ctggcctggt agcgccaggc cactcaggga cgccctgttt
      901 tttgcctacg gtcatcccga caggtcaaaa ctgctcttca ccgaggcctt gatcgccacc
      961 gggacgaagg aagccctgga tcccgcgggg gagatcgtga tggactctat agaccgtatc
     1021 cccgacgagc cccttgccca caggctcatg tcggtcatcc acgaggcctc ggggaggacc
     1081 ctggaagcgg tggcctccct tgatgtggcc ctggagctgg aacccaggaa caccggggat
     1141 tggcgcaggc tcgcactgtt gctggagcag gtgggggaga aagaaagggc tgaggggatc
     1201 ttccgggaca tccttgagga ggaccccacg ggggaggccg gcatcaggct ggccatggcc
     1261 gctgacgaca aaggtgacca ggaagaggcg gaggcgcttt accgcaaggc ctgggaagcc
     1321 ggccttcgcg gagaggagag gtccctttgc gcgaggaggc ttggttccct ttcggccgct
     1381 tcgggccgtt tcgatgaggc cctggagttt ttcgccgaag tcggcggccc ggcgggcgac
     1441 ccgcgggccg ctttctggca tgcccggtcc ctgattgagg atgaacgatg gcaggaagcg
     1501 gaaaacctcc tcgtgactct ctacgatgac cccgacctgg acagccccag ggttttctgg
     1561 ctgggccgtc ttttcctggc gaggaaagac ctgttcagcg ccaggaacat caagtgggat
     1621 ggcctcgcaa acccgtacat gcggctggta aaaggcgtcg tggaggcttt ttcaggggac
     1681 gacagggcta cggagaccct ctcggacctg cccgctccgg gcatgggggg ggatgccaaa
     1741 gccatgctgt gttcggccag ggtggccctg aaggactggg agggcgcgaa aaggttcggc
     1801 aggatggccc tggagggcgg caaggggccc tgcctgtggg aaaggcagac aagaaggccc
     1861 cttgaagagg ccggttacat gacggcgatc gcggaagccc acctggggga ctgggcggcg
     1921 gccagggacg gtttcaggag gagcgcctcc agcctgaggc accccggccc ggttttctca
     1981 ctgggtgtat ccctggtggc ccttgggaag atcgacgagg ccagggtcat cctgacgcag
     2041 ttgaaacccg cttccgccgt tctggccaca aaactggagg agcttatcgt gcagaacgtc
     2101 gggatcagga agatgctggc cgaccccgtc gacccggttt tactggacct ttacgcctca
     2161 ttatgatgtg atctggtatg atctcactct ttagactcgg ggaggaggct tttgatcatg
     2221 aagaaagtgg atagagagga agcgatggcc cttttacggg agcataacag gcagatcggc
     2281 catatccgcc atgcccttgc cgtggaggcg gccatgagat tttttgccag ggcgaacgga
     2341 gaggatgagg aagaatgggg cctcgccggg ctcgtccatg atatcgactg ggagaccacc
     2401 atcgacgatc cctccaggca tacccatctc ggagcctcct ggttgaggga cgcggggtac
     2461 cccgataata tcgtcagggc tgtgttggcc catggttggg gcatctgttc cgatgtggag
     2521 cccctctccc cgatggagaa acacctgtac gccatcgatg agctgaccgg cttcgtcacc
     2581 gccgtggctc tggtcaggcc ggggaagtcc cttcaggacc tgaccgtcaa gtccgtcagg
     2641 aagaagtgga aggacaaggc tttcgccagg ggggtcaaca gggaggtcat cgaaaaggga
     2701 acggccatgc tggaaaaacc cctggaggaa ctggtcgagg gtgtgataga agccctgagg
     2761 cctattgaga aagatatcgg tctcggggag gaggattagt cggccaaggc cgagtcgagg
     2821 agccgccact cgaggttccc gtcgtcatct tcatcaaaac gaaggagcat gaagcccccg
     2881 atatgcccgt cctccgccca ggcgacgcac cacctgggcc ctgcgaacca tacgagggaa
     2941 gggtcgtaga tcttcatggt accgccatgg atcccctccc agggaatgag gtcatccctg
     3001 gccagaag
//
LOCUS       DAOSRG010000139         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29776_length_2994_cov_6.332766, whole genome shotgun sequence.
ACCESSION   DAOSRG010000139 DAOSRG010000000
VERSION     DAOSRG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29776_length_2994_cov_6.332766"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLM30_05200"
     CDS             complement(<1..392)
                     /locus_tag="PLM30_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806563.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOR54601.1"
                     /translation="MSAGSQEEEKATPSVEKREREGTGEAPKRKIGITETALRDAHQS
                     IMATRLRTEDMIPVAERMDEVGYHSLEVWGGATFDTCMRFLEEDPWERLRTLRKRFRK
                     TKLQMLLRGQNIVGYRHYADDVVREFVK"
     gene            complement(437..1237)
                     /gene="mazG"
                     /locus_tag="PLM30_05205"
     CDS             complement(437..1237)
                     /gene="mazG"
                     /locus_tag="PLM30_05205"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOR54602.1"
                     /translation="MVRTSRIHEEMDGLLSIMERLREPGGCPWDREQTLESLRPFILE
                     EAYELVDSIERGDPDDICEESGDLLLQVVFVAQVCMEKGLFDLADSIRGLNEKLVRRH
                     PHVFGGERLETGSAVSRNWDLIKQGERRVKREDPSLFAGIPPSLPALVKSRQIQERAA
                     KVGFDWEEGDIGPIVDKIHEELKELNEAIASRDREHIEEEMGDVFFALANLSRHLKVE
                     PEFSLQRANRKFESRLRFMENRVERSGRPWSDYTLDELESLWDDAKGE"
     gene            complement(1237..>2994)
                     /locus_tag="PLM30_05210"
     CDS             complement(1237..>2994)
                     /locus_tag="PLM30_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOR54603.1"
                     /translation="AWKKASLKAREKAREAARQILALYAERETSRKEPLPPDGELVEK
                     FESAFPFEETSDQLSAIHSIKMDMEDHLPMDRLLVGDVGFGKTEVAFRAAVKAAEAGR
                     QTAFLVPTTLLAFQHFESFSARVTGLPVRAEVLSRFVSRKRQEKIKVDIEKGLVDVVF
                     GTHRLLQGDLKFRRLGLIVIDEEHRFGVLHKERLKKLDPSIDVLSISATPIPRTLHMA
                     LGGISHISMISSPPYRRQPVMTYVGPWRDELFREAVLKESARGGQVFFVHNRIETIDA
                     VAARTRSIFPGLRVDVVHGRMEEKKLEEAMIKFTRRETDLLVCTTIIESGLDLPGANT
                     LIVDNAQDLGLAQMYQLRGRVGRREEQAFAFFFYPPDRVMPYEALERLEAIAGLSDRG
                     GGFALAKRDMEIRGGGQLIGTRQHGHIDRVGFNAYFKMLEEEVTSESGGEPAQGVRME
                     IRIPVILPSSYVPQSSVRIALFRRLLRVEGVGEVLDLEKEIRERFGPIPRSVAFMLAS
                     AKVRSTGRRNGLQYVHCALEETRAGGDPSKLRELASGKAGWMLASSGILTGPGGYRGM
                     VELAEMLEAPHQGEAWGDK"
BASE COUNT          532 a          976 c          802 g          684 t
ORIGIN      
        1 cttttgacga actccctcac cacatcgtcg gcgtagtggc ggtagcccac gatattctgc
       61 ccccgaagga gcatctggag ttttgtctta cggaacctct ttctcaacgt ccggagccgt
      121 tcccagggat cctcctcgag gaaacgcatg caggtatcaa aggtggcacc cccccaaact
      181 tcaagggagt gatatcccac ttcatccatc cgttccgcca cggggatcat gtcctcggtc
      241 cggagccttg tcgccatgat ggattggtgg gcgtccctta gggccgtctc ggtgatgcct
      301 atctttctct tcggcgcctc cccggtaccc tctctctctc gtttctccac cgagggggtg
      361 gccttctcct cttcctgtga tcccgcgctc atgtcgtcac ctccgacgaa aagcctttta
      421 cctgccgctt tcactctcac tctcccttgg cgtcgtccca gagcgactcc agttcatcaa
      481 gggtatagtc cgaccagggc cttccgctgc gctcaaccct gttttccatg aaacggagtc
      541 gggattcaaa tttcctgttc gccctctgga ggctgaactc aggctccact tttaaatgcc
      601 tggacaggtt ggccagggcg aagaaaacat cgcccatctc ctcctcgata tgttccctgt
      661 cccgggaggc gatggcttcg ttgagctcct tcaactcctc atggatcttg tcaacaatgg
      721 gtccaatgtc tccttcttcc caatcaaaac ccaccttggc cgccctttcc tgtatctgtc
      781 tggatttgac caatgccgga aggctggggg gtatccccgc gaagagggag ggatcctcgc
      841 gcttgaccct tctttcaccc tgcttgatga ggtcccagtt cctgctgacg gcggaaccgg
      901 tctcaagtct ctcgccgccg aagacatggg ggtgcctcct cacaagcttt tcgttgaggc
      961 cccttatgga gtcggccagg tcgaaaaggc ctttctccat gcatacctgg gcgacaaaaa
     1021 ccacctggag gagcagatcg ccgctttcct cgcatatgtc gtccgggtca cccctctcga
     1081 tggagtccac cagctcataa gcctcttcta gtatgaaggg cctgaggctc tccagggtct
     1141 gctccctgtc ccaggggcaa ccgccgggtt cccggagcct ttccattatt gaaagcagcc
     1201 catccatctc ttcgtgaatc ctcgatgtcc tgaccattac ttatctcccc atgcttcacc
     1261 ttgatggggg gcttcgagca tttcggccag ttcgaccatg cccctatacc cccctggccc
     1321 tgtgaggatg ccactcgacg ccagcatcca acccgcttta ccggaagcga gttcccggag
     1381 ttttgagggg tcacctccgg cccgggtctc ctccagggcg caatgaacgt attgtaaccc
     1441 attgcggcgc cccgtggacc ggaccttcgc cgaggccaac atgaacgcca ccgatcgggg
     1501 tattgggccg aagcgttccc tgatctcctt ctccagatcg agcacctccc ctactccctc
     1561 tacccgcagg agcctcctga agagggctat cctgacgctg gattggggta cgtaactgct
     1621 tgggaggatg acggggatac ggatctccat cctcaccccc tgggcgggtt ccccgccgga
     1681 ttcggaggtg acctcctcct cgagcatctt gaagtaggca ttgaaaccca ccctgtcaat
     1741 atgtccatgc tggcgggtcc cgatcagctg gccgccgccg cggatctcca tgtccctttt
     1801 cgcgagggcg aaaccgcctc ccctgtcgct caggccggcg atagcctcga ggcgttcaag
     1861 ggcttcatag ggcatgaccc tgtcgggagg gtagaagaaa aaagcaaagg cctgttcttc
     1921 ccttcttcca accctgcctc tcagttggta catctgggcc aggccgaggt cctgggcgtt
     1981 gtccacgatg agggtattgg caccgggcag gtcgaggccg ctctcgatga tggtggtgca
     2041 gacgaggagg tccgtttccc tcctggtgaa cttgatcatg gcctcttcaa gctttttctc
     2101 ttccatccgg ccgtggacga catccaccct gaggccggga aagatcgacc tcgtcctggc
     2161 ggcgacggca tcgatggtct cgatcctgtt atggacgaaa aagacctgcc cccccctggc
     2221 actctccttc aggacggcct ccctgaaaag ttcgtccctc cagggcccca cataggtcat
     2281 gacgggctga cgcctgtagg ggggcgacga tatcatggag atatggctta tcccccccag
     2341 ggccatgtgg agcgtcctcg gtatgggcgt ggccgagatg cttagcacgt cgatggacgg
     2401 gtcaagtttc ttcagccgtt ccttgtgcag gacgccgaaa cggtgctcct cgtcgatgac
     2461 gatcaagccg agcctcctaa actttaggtc gccctggagg agtcggtgag tcccgaagac
     2521 cacgtccaca agccccttct cgatatctac cttgatcttc tcctgccttt tacgcgaaac
     2581 gaaccgggag agcacctcgg ctctcaccgg gaggcccgtt accctggcgg aaaaggattc
     2641 aaagtgttga aaagccagga gcgtcgtggg gaccaggaaa gctgtctgtc gccccgcttc
     2701 ggcggccttc acggccgccc tgaaagcgac ctcggtcttc ccgaaaccca catcgcctac
     2761 gagaagcctg tccatcggga ggtggtcctc catgtccatc ttgatcgagt ggatggcgga
     2821 cagctggtcg ctggtctcct caaaggggaa agcgctctcg aacttctcca ccagctcgcc
     2881 atcggggggc aaaggttcct tcctgctcgt ctccctctcg gcgtagaggg ccagtatctg
     2941 gcgggcggcc tccctggcct tctccctcgc cttgagagaa gcctttttcc acgc
//
LOCUS       DAOSRG010000140         2992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29797_length_2992_cov_9.431393, whole genome shotgun sequence.
ACCESSION   DAOSRG010000140 DAOSRG010000000
VERSION     DAOSRG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29797_length_2992_cov_9.431393"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="def"
                     /locus_tag="PLM30_05215"
     CDS             complement(<1..428)
                     /gene="def"
                     /locus_tag="PLM30_05215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOR54604.1"
                     /translation="MAVRDIREYPEQVLRKGSQDVTDFGEDLQSLLRDMWETMVSNDG
                     VGLAAPQIGVSLRVAVIGWRDRRIVLVNPVVIEQEGEEELEEGCLSAPGFFEKVTRPA
                     RVVVEAQDEHGEPIRLEEEGFLARVLMHEIDHLEGKLFLDR"
     gene            complement(499..2007)
                     /locus_tag="PLM30_05220"
     CDS             complement(499..2007)
                     /locus_tag="PLM30_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOR54605.1"
                     /translation="MTEETGTNNEKAAVPSNEGNHESMENFLEKYSVGAFHRGKTVEG
                     TVVAVNDEGWLVDVGFKCEGFLPRNEWSHRSLVEAKEAPSVGDPVRVRVVSLKQGEES
                     QLVVSRWRIEFDERWDAMESSLAEGDVLEVRGLRKVKGGLIVDCCGIEGFVPVSHLAE
                     EGRGVNPSKLVDDTFSVKVLEKDRRKRRIVLSRRALLEEELQVDKQAFFEETEIGQTM
                     EGVVTSVTSFGAFVNLGPVEGLVHNTELAWGRNVKPKEIVNKGDRVNVRIIGIDPESS
                     RVSLSIKQTQPDPWDDVAARYEEGQVLTGRITNLADFGAFVELEPGVEGLVHIGDISW
                     QRIGHPREVLKKGQKVETQILSVDGENRRISLGYKQLHDPWKDLGEKYEVDGTYTVKV
                     VRLTDFGAFVELEDGIEGLIHVSQISRKRVEKPGDVVSEGQEVQARIIEIKTGERRIR
                     LSMSALEEPEPQGVKEPAKRRKKGDHRDDPSQFLDGETNVTIGDIIKESLQE"
     gene            complement(2007..2867)
                     /gene="ispH"
                     /locus_tag="PLM30_05225"
     CDS             complement(2007..2867)
                     /gene="ispH"
                     /locus_tag="PLM30_05225"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR54606.1"
                     /translation="MELIFAEPKGFCFGVRRAITALEKSLEENGKVYALGSPIHNPQE
                     VERLLSKGLEIIETPSEMPPGGTVFVRAHGIPDQVMGELRARKARVLDGTCPFVSRAK
                     EKAALLAREGYTVMVLGDKDHPEVRAIRRSSEGSVIVVDPGSVAGLTCDGLSKVGIVS
                     QTTQRKEVLAELVTRVSAGAGETRVFNTICDATSSRQASVARLAGSVDGIIIIGGKDS
                     ANTARLFQIAAASGCPALWVEDETGLERGWLKGKKRIGIAAGASTPDWLIQKIATILE
                     SVGSAGEDGR"
BASE COUNT          491 a         1030 c          801 g          670 t
ORIGIN      
        1 cggtcgagga acagtttgcc ttccaggtgg tctatctcgt gcatgagcac cctggccagg
       61 aagccttcct cctcaagcct gatgggttcg ccgtgctcat cctgggcttc gacgacaacc
      121 cgggccggcc gcgtgacctt ttcgaaaaac cctggcgcac tcaggcaacc ttcctccagt
      181 tcctcttcac cctcctgttc gatcacgaca gggttgacca ggacgatcct cctgtccctc
      241 caaccgatga cggcgaccct caggctgacc ccgatctgcg gcgccgccag gcccacgccg
      301 tcgttcgata ccatcgtctc ccacatatcc cgcagaaggg actggagatc ctccccgaaa
      361 tcggtcacgt cctgggatcc ttttctcagg acctgctcgg ggtactcccg gatatccctg
      421 acagccatgg tgccatcccc tcgccttccg ggccttaaaa agcggccctt cctggaaggg
      481 ccgcccggct cgtcgatatt attcctgcaa tgactccttg atgatgtcac cgatggttac
      541 attggtctcc ccgtcgagga actgggaggg gtcgtcgcga tgatcgccct tcttcctcct
      601 cttcgccggc tccttcacgc cctgcggttc gggttcctcg agagcgctca tgctgagcct
      661 tatcctgcgc tccccggttt ttatctcgat gatcctggcc tggacctcct gtccctcgga
      721 gaccacgtcg cccggttttt cgacccgttt acggctgatc tgggagacat ggatcaaacc
      781 ctctatgcca tcctccagtt ctacgaaggc cccgaaatca gtgaggcgga ccaccttcac
      841 ggtgtaggtc ccgtcgacct cgtacttctc ccccaggtcc ttccaggggt cgtggagttg
      901 cttgtaaccc aggcttatcc tcctgttttc gccatcgacg gagaggatct gggtttccac
      961 cttttggccc ttcttgagga cttcccgggg gtgaccgatc ctctgccaac tgatgtcgcc
     1021 gatatggaca agcccctcga ccccgggttc cagttcgacg aaagcgccga aatcggcgag
     1081 gttggtgatc ctgccggtga ggacctgccc ctcctcatag cgggcggcca catcatccca
     1141 cgggtcgggc tgggtctgct ttatgctcag ggacacccgg gagctctcgg ggtcgatccc
     1201 gatgatcctg acgtttaccc ggtcgccctt gtttacgatc tctttgggct tgacgttcct
     1261 cccccaggcg agttccgtgt tgtgaacgag gccttccacg gggcccaggt tgacgaaggc
     1321 gccgaaggag gttacggagg tcaccacgcc ttccatggtc tgcccgatct cagtctcttc
     1381 gaaaaaggcc tgcttgtcca cctggagttc ctcctccagc agggccctcc gggagaggac
     1441 gatcctccgc ttccgccggt ccttttcaag gaccttgacg ctgaaggtgt cgtcgaccag
     1501 tttcgagggg ttcacacccc tgccctcctc ggcgaggtgc gatacgggga cgaaaccctc
     1561 tataccgcag cagtcgacga tgaggccgcc cttgaccttt ctcaggcccc tgacttccag
     1621 cacgtcccct tcggcgaggc tcgattccat ggcgtcccac cgttcgtcga actcgatcct
     1681 ccatcggctg acgaccagtt gggactcttc gccctgtttc agggagacca cccgcaccct
     1741 gacggggtca cccacggaag gggcctcctt ggcctccacc agggaccggt ggctccattc
     1801 attccttggg agaaaaccct cgcacttgaa acccacgtcc accagccatc cctcgtcgtt
     1861 gacggccacc accgtgccct cgacggtctt gccgcggtga aacgcgccta cactgtactt
     1921 ttccaggaag ttctccatcg attcatggtt cccctcgttt gaggggactg cagccttttc
     1981 gttgttcgtt cctgtttctt cagtcattac cgaccatcct ccccggcgct cccgacagac
     2041 tcaagtatcg tggcgatttt ttggatcagc cagtcgggcg tgctcgcacc ggctgcgatc
     2101 ccgatccttt tctttccctt caaccatccc ctttcgagcc cggtctcatc ttcgacccag
     2161 agggccgggc atcccgacgc ggccgctatc tggaagaggc gcgccgtgtt ggcgctgtcc
     2221 ttcccaccga taatgataat accatcaacg gaaccggcaa gtcgggccac cgatgcctgc
     2281 ctcgaggagg tggcatcgca gatggtattg aaaaccctcg tctcccccgc cccggcggac
     2341 acacgggtga ccagttccgc caggacctcc ttgcgctggg tggtctggga gacgatgccc
     2401 accttgctca gcccgtcgca ggtcaggccc gcgacggacc cggggtccac cacgatgacg
     2461 gaaccctcgg aggatcgcct tatggccctc acctcggggt ggtccttgtc gccgagcacc
     2521 atgacggtgt agccttcccg ggcgagcagg gcggccttct ccttcgccct gctgacgaag
     2581 gggcaggtac cgtccaggac ccgtgccttc ctggcccgca gctcccccat gacctggtcg
     2641 ggtataccgt gggcccggac gaacacggtt cctcccggcg gcatttcgga aggggtttca
     2701 atgatctcta gaccttttga gaggagtctt tccacttcct ggggattatg gatcgggctt
     2761 cccagggcgt agaccttgcc gttctcttcc agggattttt ccagggcggt aatggccctt
     2821 ctcaccccga agcagaaacc cttcggctcg gcgaagatca gctccacccc agtcgttccc
     2881 cctgtcttgc ttcccaaaga cacaccactt tcctggcgat ttcagggatc gatccgacgg
     2941 tgacataata ccacaggcac ggggtaaacc tcctgaacca ggtcatctgc ct
//
LOCUS       DAOSRG010000141         2992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_29798_length_2992_cov_9.296221, whole genome shotgun sequence.
ACCESSION   DAOSRG010000141 DAOSRG010000000
VERSION     DAOSRG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29798_length_2992_cov_9.296221"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLM30_05230"
     CDS             complement(<1..589)
                     /locus_tag="PLM30_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOR54607.1"
                     /translation="MMNMDGKLDELERSLEEIERRMSEPSVGSNPGELQRLGKKRSEI
                     VGIVEVYREYRNTRTRLEEARELTHSGDPELSELAREETRELEPKMEELESRLQVLLL
                     PKDPNDDKSVIVEIRAGTGGEEAALFAGDLFRMYSLFAESNGWGIEVLNTNETGIGGY
                     KEVVFRIDGQGAYSQLKFESGVHRVQRVPVTESSGR"
     gene            complement(589..1527)
                     /locus_tag="PLM30_05235"
     CDS             complement(589..1527)
                     /locus_tag="PLM30_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOR54608.1"
                     /translation="MLKALHSLSLVLAYLLSAREIPIGGQAVIEGVLMRGPGSWALAV
                     RAPGGDIWKKKWGSQPWNSPFPWNLPVLRGLATMAEMLVTGFRAISLSAQVALGEDEQ
                     IGPLELIVTFAVALLGVVGLFILLPVWASDLGVKYLGMSPFWGNIMEGLARAGVFVAY
                     IAIIGLWKDIARVFAYHGAEHKTINAWESGAPLDKEQVSRFSRIHSRCGTSFLLVVVA
                     VSVLVFALAGKGTVLWRVASRLLLLPVVIGVSYEVIRWCSKSGTLGRVLMAPALWLQY
                     LTTREPDLGQIEVALAALDLALEEGNVPRCPVEGEN"
     gene            complement(1521..2216)
                     /gene="thyX"
                     /locus_tag="PLM30_05240"
     CDS             complement(1521..2216)
                     /gene="thyX"
                     /locus_tag="PLM30_05240"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOR54609.1"
                     /translation="MPISVLLLANTPEPDRTVAAAARLCYSDVSAVEIAEGLKGEDVS
                     RFIGHLRSSGHFSPFEHAAFTFAIDGISRVCSHQLVRHRMASYSQQSQRYVPMEEPEM
                     ITPPSLSSNPEALALFTKVAAEAHDAYGKMIGSGIPAEDARYILPHGWATKMVVTMNA
                     RELHHFFSMRLCRRAQWEIRDLARRMLILAREKAPVLFSLAGPDCLTKGRCFESRPCG
                     KPFSSMEEVLSGC"
     gene            complement(2241..2489)
                     /gene="rpmE"
                     /locus_tag="PLM30_05245"
     CDS             complement(2241..2489)
                     /gene="rpmE"
                     /locus_tag="PLM30_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOR54610.1"
                     /translation="MKKDIHPKYGECKVTCACGNSFVTGSTVEEIKVAVCSACHPVFT
                     GKRGTRVSEAGRVEKFQKKYAGTRFGKAPESEEEEKKD"
     gene            complement(2585..>2992)
                     /locus_tag="PLM30_05250"
     CDS             complement(2585..>2992)
                     /locus_tag="PLM30_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HOR54611.1"
                     /translation="DDSQIGRSALILASTTGPEEERVLKEQIAGFGWKAVATEVGGLA
                     GDLPQKITRAVVGAALNGEIVRKNANEMHALMHAAFEAMNGFISVGMLETSIGMKIGI
                     VRNRHWIAVAVVGDSAYHAVAHHERCGLGVMHI"
BASE COUNT          567 a          969 c          863 g          593 t
ORIGIN      
        1 tcctcccgct cgattcggtc acggggaccc tctggaccct gtggacgccg ctttcgaatt
       61 tgagctggct gtaggccccc tggccatcga tcctgaagac aacctccttg tacccgccga
      121 taccggtctc gttggtgttc aggacctcga tgccccaccc gttgctctcg gcgaagaggg
      181 agtacatccg gaagagatcc cccgcgaaga gggcggcctc ttcgccaccg gtcccggccc
      241 tgatctccac gatgacgctc ttgtcatcgt tggggtcctt cgggagcaac agaacttgga
      301 gcctcgactc cagttcctcc attttcggct cgagttcccg cgtctcttcc cgggccagtt
      361 cactgagttc ggggtccccg gaatgggtga gttccctggc ttcttccagc ctcgtccggg
      421 tattcctgta ttccctgtag acctccacga tgcctacgat ctcggagcgt ttttttccga
      481 gccgctggag ttctcccggg tttgacccta ccgagggttc ggacatcctg cgctcgatct
      541 cctcaaggga tctttcgagt tcgtccagct tcccatccat gttcatcatc aattttcacc
      601 ttccacgggg cacctcggga catttccctc ctcgagggcg aggtccagcg ccgccaaagc
      661 cacttcgatc tgccccaggt ccggctccct cgtggtcagg tactgcagcc agagggccgg
      721 ggccatcagg acccttccca gggtgccgct cttcgagcac caccggatca cctcgtagga
      781 aacgcctatc accacgggca ggagcaacag cctcgaggcg acccgccaca gtacggtccc
      841 ctttccggcc aaggcgaaga ccaggacgct cacggcgacc accaccagga gaaaggaagt
      901 gccgcaacgg gagtgaatcc tggaaaaccg ggacacctgt tccttatcaa ggggggctcc
      961 cgactcccag gcgtttatgg tcttatgttc cgccccgtgg taggcgaaca ccctggcgat
     1021 atccttccaa agccctatga tggcgatgta agccacgaag accccggccc tggccagccc
     1081 ttccatgata ttcccccaga aagggctcat ccccaggtac ttgaccccca ggtcggaggc
     1141 ccagacgggg agcaatatga agagccccac taccccgagc agggccacgg cgaaggtgac
     1201 gatcagctcg aggggaccta tctgctcgtc ctctcccaac gctacctggg ccgaaaggga
     1261 gatggccctg aaaccggtga cgagcatctc ggccatcgtc gcaaggccgc gaaggaccgg
     1321 gaggttccag gggaaggggg agttccaagg ctgggatccc catttcttct tccagatgtc
     1381 ccccccgggg gctctcacgg ccagagccca gctgccgggg cccctcatga gaacgccctc
     1441 gatgaccgcc tggcctccga tgggtatctc cctggccgaa agaagatagg ccaggacgag
     1501 ggaaagggaa tgaagggcct ttagcatccg gagaggacct cttccatgct tgaaaagggt
     1561 ttaccgcagg gcctgctttc aaagcatcga cccttggtaa ggcaatcggg gcccgccagc
     1621 gaaaaaagca cgggcgcctt ctcccgcgcc aggatcagca tcctgcgggc gagatcccgg
     1681 atctcccatt gcgccctgcg gcataggcgc atcgagaaga aatgatgcag ttcgcgggcg
     1741 ttcatcgtca cgaccatctt cgtagcccaa ccatggggga gtatataccg ggcatcctca
     1801 gcgggaatac cggacccgat catcttcccg taagcgtcgt gggcctcagc ggcgaccttc
     1861 gtgaagaggg ccagggcctc agggttggag gacagggacg gcggggttat catttccggc
     1921 tcttccatgg gaacgtaacg ctggctctgc tgggagtagc tggccatcct gtgcctgacc
     1981 agctggtggg aacagaccct gctgatcccg tcgatggcga aagtgaaggc cgcgtgctcg
     2041 aagggagaga agtgccccga ggacctcagg tggccgatga aacgggatac atcctctccc
     2101 ttgaggcctt cggctatctc cacggcggag acgtcgctgt agcagagcct cgcggcggcg
     2161 gctacggtcc tgtcgggttc aggtgtgttc gccaacagca ggacactgat cggcatggat
     2221 tcacctctat agtgcggtta tcagtctttc ttctcttctt cctcagactc cggggccttt
     2281 ccaaagcggg taccggcgta cttcttctgg aacttttcga ccctgccggc ctcggacacc
     2341 ctggtgcccc gtttgcccgt aaataccggg tggcaggcgg aacaaacggc taccttgatc
     2401 tcctcgaccg tggagccggt cacgaaggag ttcccgcaag cgcaggtcac cttgcattca
     2461 ccatacttcg ggtggatatc ctttttcact gagcgcacct ccatggagaa acgatctttt
     2521 cttgccacaa ggaaggaatg taacacaggg ctccggaaaa tacaataagg gcggcctcca
     2581 caggtcagat atgcattacc cccagcccgc accgctcatg gtgggcgacg gcatggtagg
     2641 ccgaatcgcc gacaaccgca acggcgatcc agtgcctgtt ccggacgata ccgatcttca
     2701 tgccgatgct cgtctccagc ataccgaccg agatgaaccc gttcatggct tcaaaagcgg
     2761 catgcatcag ggcgtgcatc tcgttggcat ttttccggac tatctcaccg ttcagggcgg
     2821 ccccgaccac ggccctggtg atcttctggg ggagatcccc ggccaggccg ccgacctcgg
     2881 tcgccacggc cttccagccg aacccggcga tctgttcttt caggaccctc tcctcttccg
     2941 gccccgtggt ggaagcgagg atgagggcgg accggcctat ctggctgtcg tc
//
LOCUS       DAOSRG010000142         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_30340_length_2952_cov_11.383845, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000142 DAOSRG010000000
VERSION     DAOSRG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30340_length_2952_cov_11.383845"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLM30_05255"
     CDS             complement(<1..1091)
                     /locus_tag="PLM30_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOR54612.1"
                     /translation="MSPGTRETIVIIDCGFQQTQLIARRVREKGVYSEVVPCGAGHEA
                     IMARDPAGLIIIGGPATVLEDDSPKVPVELLDGKVPVLGVCYGMPLIASLLGGEVEGG
                     EPVGFGRGMILVEGGGGALLQGFPAETEVWVSRWDRVLSPPPGFTVTARSESGTIAAF
                     EGPGSRVSAVAFLPEAPQDPGGADILSSFLFNVCRCRKAWDLGEWVETTVGEMKSAVG
                     DGRVVCGLSGGVDSTVAAVLASKAVGDRLECIFVNSGLLRLDEAEQVLETYRGSLGLK
                     VHYVDASDDFLSALKGVTDPEAKRKAVGETFIRVFERKAREIGGASWLLQGTIYPDVI
                     ESGPRGKGTPAVKSHHNVGGLPQEMDFQLL"
     gene            complement(1192..2820)
                     /gene="groL"
                     /locus_tag="PLM30_05260"
     CDS             complement(1192..2820)
                     /gene="groL"
                     /locus_tag="PLM30_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162755.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOR54613.1"
                     /translation="MPKILSFGEEARRALERGINKVTDTVGSTLGPKGRNVVLEKKFG
                     SPTITNDGVTIAKEIELEDPFENMGAQLLKEVASKTNDVAGDGTTTATVLARAIILEG
                     MKNVAAGANGALMRHGIEKAVDLVVDELKAMAHNVKEREKIAQVAAISANDRAVGELI
                     AEAMEKVGEEGVITVEDSQTVGTTLEMVEGLQFDKGYVSPYMMTDPERMEAHLEDVYI
                     LINDGKVNSVKDMVPLLEKVVQTGKPLLIIAEDVEGEALATLVVNKLRGTMQVVAVKA
                     PGFGDRRKAMLQDISIVTGGQVISEEIGLKLENADLSMLGKAKKVRVTKEETTIVEGA
                     GDPDAIRKRAAQIKAELEESTSEYDKEKLQERLAKLVGGVAVVQVGAATETEQKELKH
                     RIEDALNATRAAVEEGIVAGGGVALINAIEPLDKFIAKLEGDEKTGATIVKKALISPI
                     HLIAANAGYQGDVVVEKVNGLKKGFGFNAMTGEYEDMIKSGIIDPVKVTRSALQNAGS
                     IASMVLTTETLMAEKPKKESEMPAMPGGMGGMDY"
     gene            complement(2869..>2952)
                     /locus_tag="PLM30_05265"
     CDS             complement(2869..>2952)
                     /locus_tag="PLM30_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOR54614.1"
                     /translation="YGGTEVKIEGEELLILSERDVLAVLEK"
BASE COUNT          462 a          978 c          890 g          622 t
ORIGIN      
        1 aggagctgaa agtccatctc ctgggggagc ccgcccacgt tgtggtggct cttgacggcc
       61 ggcgttccct taccgcgggg gccgctctcg atcacgtcgg ggtagatcgt gccctgcagg
      121 agccaggagg ccccccctat ctcccgagcc ttgcgctcga agaccctgat aaaggtctcg
      181 ccgacagcct tgcgcttcgc ctcggggtca gtcaccccct taagggccga gaggaaatca
      241 tcggaggcgt ccacgtaatg caccttaagc cccagggatc cccggtaggt ctccaggacc
      301 tgctcggcct catccagccg gaggaggccg ctgttgacga agatgcattc caggcggtcg
      361 cctacggctt tcgaagcgag gacggcggcc acggtcgagt ctacgccgcc ggagagtccg
      421 cagacgacac ggccgtcgcc tacagcgctc ttcatctccc ccacggtcgt ttctacccac
      481 tccccgaggt cccaggcctt ccggcaccgg cagacattga aaaggaagct ggagaggatg
      541 tcggcaccgc ccgggtcctg gggcgcctcc ggaaggaagg ccacggccga aaccctggaa
      601 cccggtccct caaaagccgc tatcgtaccg gactcgctcc ttgcggtgac cgtgaacccc
      661 ggaggcggcg acaggacgcg gtcccagcgg ctcacccaga cctccgtttc ggcggggaat
      721 ccctgcaata gggcccctcc tccaccttct accaggatca tgcccctccc gaacccgacg
      781 ggctcaccac cttcgacctc gccgcccagg agactggcga tcaacggcat accgtagcag
      841 acgcccagga ccgggacctt cccgtcgagc agttccacgg ggaccttggg cgagtcatct
      901 tcgaggaccg tcgccgggcc cccgatgatg atcagccccg ctgggtccct ggccatgatg
      961 gcttcatgac cggcgccaca ggggaccacc tcgctgtaga cacccttttc ccggaccctc
     1021 ctggcgatca gctgggtctg ctgaaaaccg caatcgatga tgacgatggt ctctctcgtt
     1081 cctggactca aaaggatcac ctctcacgac acaagtttat cagaaccggg acggggcgct
     1141 cacaatgaaa aaagggcccc cgcgggggcc ctttctatac tagagggtct atcaataatc
     1201 catcccgccc atgccgccgg gcatcgcggg catctcgctc tccttcttgg gcttttcggc
     1261 catgagggtc tccgtagtga ggaccatgga ggctatggag ccggcgttct ggagggcgct
     1321 cctggtgacc ttgaccgggt cgatgatgcc ggacttgatc atatcctcgt actcgccggt
     1381 catggcgttg aagccgaagc ccttcttgag gccgttgacc ttttcgacca ccacgtcgcc
     1441 ctggtacccg gcgttggcgg cgatcaggtg gatcggcgag atgagggcct tcttgacgat
     1501 ggtcgcgccg gtcttctcgt cgccttcgag tttggcgatg aacttgtcga ggggctcgat
     1561 ggcgttgatc agggccacgc ctcccccggc cacgatgcct tcttcgacgg cggcccgggt
     1621 ggcgttgagg gcgtcctcga tccggtgctt cagttccttc tgctccgtct cggtggcggc
     1681 cccgacctgg acgacggcca ctccgcccac cagcttggcc aggcgctcct ggagcttctc
     1741 cttatcgtac tcggaggtgg attcctccag ttcggccttg atctgggcgg cgcgcttcct
     1801 gatggcgtcg gggtcgccgg cgccttcgac gatcgtggtc tcttccttgg tcaccctgac
     1861 cttcttggcc ttgccgagca tggagagatc ggcgttctcg agcttgagac cgatctcctc
     1921 ggagatcacc tgtccgccgg tcacgatgga gatatcctgg agcatcgcct tgcgcctgtc
     1981 gccaaaaccg ggggccttga cggcgacgac ctgcatcgtc ccgcggagct tgttgaccac
     2041 gagtgtcgcg agggcttcac cctcgacgtc ctctgcgatg atcagcaggg gcttgccggt
     2101 ctggaccact ttctcgagga gggggaccat gtccttgacg ctgttgacct tgccgtcgtt
     2161 aatgaggatg tagacgtcct ccaggtgggc ttccatgcgc tcgggatcgg tcatcatgta
     2221 ggggctcaca tagcccttgt cgaactggag tccctcgacc atctccaagg ttgtgccgac
     2281 ggtctggctg tcctcgacgg tgatgacgcc ctcttccccg accttctcca tggcctcggc
     2341 gatcagttcg ccgacggcgc ggtcgttggc ggagatggcg gccacctggg cgatcttctc
     2401 ccgctccttt acgttgtggg ccatcgcctt gagttcgtcg accaccaggt cgacggcctt
     2461 ctctatgccg tgccgcatga gggcgccgtt ggcgccggcg gccacgttct tcatgccctc
     2521 aagaatgatg gccctggcga ggacggtagc ggtcgtggtg ccgtcgccgg ccacgtcgtt
     2581 cgtctttgag gccacttcct tgagcagctg ggcccccatg ttctcgaagg ggtcctcgag
     2641 ttcgatctcc ttggcaatgg tcacgccgtc gttggtgatc gtcggcgagc cgaacttctt
     2701 ttcgagcacc acgttgcggc ccttgggccc cagggtgctc cccacggtat cggtgacctt
     2761 gttgatcccg cgctcaaggg ctctgcgggc ttcttcacca aaagaaagga tcttaggcat
     2821 ttctgttctt cctccttcct ggctcttcag gaaaattatt tgtccggact acttctcaag
     2881 gacggcaagg acgtcgcgct cgctgaggat gagcagttcc tcgccttcga tcttgacttc
     2941 ggtgccgccg ta
//
LOCUS       DAOSRG010000143         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_30783_length_2920_cov_8.315532, whole genome shotgun sequence.
ACCESSION   DAOSRG010000143 DAOSRG010000000
VERSION     DAOSRG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30783_length_2920_cov_8.315532"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLM30_05270"
     CDS             complement(<1..430)
                     /locus_tag="PLM30_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54615.1"
                     /translation="MSGLLSLEGVRVTLGSREVLKEINLEVAAGQVVALLGPNGSGKS
                     TLLAAAAGIGRKDAGRVLVEGDDLDALTRREAARRIAVVPQQTGFMTPFTVMETVLMG
                     RFPSAGRFSRLTAGDRRAAEASLEAVDLSSFGERLVTDLSG"
     gene            complement(427..1398)
                     /locus_tag="PLM30_05275"
     CDS             complement(427..1398)
                     /locus_tag="PLM30_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOR54616.1"
                     /translation="MSFRGGLALPLVILSALLAVALLWVITVGEVPIPGGDVLGVITG
                     RVPASDPRSKIVLSIRLPRAFAASSAGAVLAGSGVIFQGVLLNPLAEPYTLGVAAGAA
                     LGASAAITLGLPFISAFAFAGSIAALWLVWLLGRGSRGRASPSRLILAGVIVGSILGS
                     VITLMEALAGEQVGAIVLWLLGSFSLSTWSHAARSAAMAAVVLVLGLFWVRDLDILSS
                     GADAASLGVDERKTSGVLLLGSSLATAMVVSMSGVIGFVGLVVPHLVRIVVGPAHRRL
                     MPLSVLGGALLLLLADTAARSLGELPVGVITALVGGPLFCFLLWRER"
     gene            complement(1395..2330)
                     /locus_tag="PLM30_05280"
     CDS             complement(1395..2330)
                     /locus_tag="PLM30_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54617.1"
                     /translation="MFILLTGDSQAGNASPGRFPSHAEADFTWWGITDPGMVLALSGG
                     SPTPPVLVTRAARFLADFPTLVVRCGSRTAPKCPYMETGTPTGGDPAKGPALPGAGVT
                     WDSGFSLGRLLAGLDDRLVIGSSCPGGEVTARLFLKALGYKAAAIEDFPANSEGPDPW
                     EAASSRLGIRPGDLIGHGFKAASELGDPAMVVAAALAAGAMGGAKVLLSGGLPMLAAA
                     ALLRDLGEKGRFSLAVTARTERDLGGAFGDLSALLDLETQTVDLGETPDGAGASGAAL
                     LAGESGFTLERVLDRASRLRGEIAGGVHPPEGGGL"
     gene            complement(2315..2911)
                     /locus_tag="PLM30_05285"
     CDS             complement(2315..2911)
                     /locus_tag="PLM30_05285"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOR54618.1"
                     /translation="MTTAYFDGASRGNPGESAAGACIIDDDGQVAWQCSRALGIGTNN
                     EAEYKALIMLLEEIAARGLKKVTVHGDSRLVISQVKGLWKVREERLRPLAERARELAR
                     TTGAVLQWVPRERNAAADALSNRAFSSPGQGPSSFPRGDLERVSSTIFIAHGTADYAV
                     DVKHRSCSCPGFRNRGDCKHLRAALSLVEEEGGDCSSC"
BASE COUNT          476 a         1000 c         1063 g          381 t
ORIGIN      
        1 cccccgagag gtccgtgacg agcctctcgc cgaaggagga gaggtccacc gcctccaggg
       61 aggcttccgc cgcacggcgg tccccggcgg tgagcctgga aaacctcccc gccgatggga
      121 agcgccccat gaggaccgtc tccatgacgg tgaagggcgt catgaagccc gtctgctggg
      181 gcaccacggc tatcctcctg gccgcctccc tcctggtgag ggcgtccagg tcgtcgccct
      241 ccaccaggac ccttccggca tctttccttc cgatacccgc agcagccgcc aggagggtgc
      301 tcttgccgct cccgttgggg cccaggagcg ccacgacctg gccggcggcg acctccaggt
      361 tgatctcttt gaggacctcc cttgaaccga gggtcaccct gacgccctcg agggagagga
      421 ggccgctcat ctttccctcc agagcaggaa gcagaacagg ggccccccga cgagggccgt
      481 gatgaccccc acggggagtt cacccaggga gcgggccgcc gtgtccgcca ggagcaggag
      541 aagggccccg cccaggacgc tcagcggcat gagcctgcgg tgggccggac cgacgacgat
      601 cctcaccagg tggggcacca cgagtcccac gaagccgatg acgccgctca tggagaccac
      661 catggccgtc gccagggaac ttcccaggag gagcacgccg gaggtcttgc gctcgtcgac
      721 gcccagggag gcggcgtcgg cgccggagga gaggatatcg aggtcgcgga cccagaagag
      781 ccccaggacc aggaccaccg ccgccatggc ggcgctccgg gcggcgtggc tccaggtgga
      841 gagggagaaa cttcccagga gccagaggac gatcgcgccc acctgctccc ccgccagggc
      901 ctccatgaga gtgatgaccg agccgaggat gctgccgacg ataacgccgg ccaggatgag
      961 gcgcgagggc gaagccctgc cgcggctgcc ccgccccagg agccacacga gccagagggc
     1021 ggcgatgctg ccggcgaagg cgaaggccga tatgaagggc agtcccagcg ttatggccgc
     1081 ggaggccccg agggcggccc cggcggccac gcccagcgtg tagggttcgg ccagggggtt
     1141 gaggagcacc ccctggaaga tgaccccgct cccggcgagg acggccccgg ccgacgaggc
     1201 ggcgaaggcc ctaggtaggc gtatggagag gacgatcttc gaccgggggt ccgaggcggg
     1261 tacgcggccc gtgatcaccc ccaggacgtc gcccccgggt atgggcacct cgccgacggt
     1321 gatgacccaa aggagcgcga cggccaggag ggccgaaagg atgacgaggg ggagggccag
     1381 cccgccccgg aagctcatag cccgcctccc tccggaggat ggaccccgcc cgcgatctcc
     1441 ccgcggagcc gggaggcccg atccaggacc ctttcgaggg tgaagcccga ctcccccgcc
     1501 aggagggccg ctcccgaggc ccccgccccg tcgggggtct cccccaggtc gacggtctgg
     1561 gtctcgaggt caaggagcgc cgaaaggtcg ccgaaggccc cgccgaggtc cctttcggtc
     1621 cgagccgtga cggcgaggga gaaccgcccc ttctcgccga ggtccctgag cagcgccgcg
     1681 gcggcgagca tgggaaggcc ccccgaaagg aggaccttcg ctccccccat ggccccagcc
     1741 gccagggccg cggcgaccac catggcgggg tcgcccagct ccgaggcggc tttgaagcca
     1801 tgaccgatga ggtcgccggg gcgaatgccc agcctcgacg atgcggcctc ccaggggtca
     1861 ggcccttcac tattggccgg gaagtcctcg atggcggcgg ccttgtaccc cagggcctta
     1921 aggaagagcc tggcggtgac ctcgccgccg gggcaggaac tcccgatgac gaggcggtcg
     1981 tcgaggccgg ccaggagcct tccgaggctg aagccgctgt cccaggtcac cccggcgccg
     2041 gggagagccg ggcccttggc ggggtctccc cccgtcgggg tccccgtttc catgtagggg
     2101 cacttcggcg cggtcctgga accgcagcga accaccaggg ttgggaaatc ggccaggaag
     2161 cgtgccgccc tggtgaccag gaccgggggg gtcggggaac cgccggatag ggcgaggacc
     2221 ataccggggt cggtgatccc ccaccaggtg aagtcagcct cggcgtggga ggggaaacgt
     2281 ccgggggagg cgttcccggc ttgggagtcc cccgtcagca ggatgaacaa tccccaccct
     2341 cctcttcgac cagtgaaagg gcggcccgga ggtgcttgca gtctccccgg ttgcgaaagc
     2401 cggggcagct gcaggagcgg tgcttcacgt cgacggcgta gtcggcggtg ccgtgggcga
     2461 tgaagatagt tgaggaaacg cgctcgaggt caccccgtgg gaaggaagat ggaccttggc
     2521 ccggtgagga gaaggccctg tttgagaggg cgtcggcggc ggcgtttctc tcccggggga
     2581 cccactggag gacggcgccg gtggtgcggg ccagctcgcg ggccctttcg gccaggggtc
     2641 ggaggcgctc ctcgcggacc ttccagaggc ccttcacctg ggagatgacc agccggctgt
     2701 cgccgtggac ggtgaccttc ttgaggcccc tcgcggcgat ctcctcgagg agcattatca
     2761 gggccttgta ctcggcctcg ttgttggtgc cgatgcccag ggcccgggag cactgccagg
     2821 ccacctggcc gtcgtcgtcg atgatacagg ccccggcggc cgattcgccc gggtttcccc
     2881 gggaggcccc gtcgaaatag gctgtagtca tgggtatccc
//
LOCUS       DAOSRG010000144         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_30900_length_2912_cov_6.787189, whole genome shotgun sequence.
ACCESSION   DAOSRG010000144 DAOSRG010000000
VERSION     DAOSRG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30900_length_2912_cov_6.787189"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLM30_05290"
     CDS             complement(<1..496)
                     /locus_tag="PLM30_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR54619.1"
                     /translation="MKKEEILLMVKEVEVQLAPFCTCEGLALHGISHLRRVSILSGRL
                     ASAVGEDVEAAVVMGFLHDCARTDDKAGDGHAHDSSVLARRLLGRFYPHLDADRICHA
                     IARHADGEVTDDGLAACLWDADRLELKRIGREIDLDLLSTEVARRLARARAARRAVLI
                     GGGHD"
     gene            complement(588..2594)
                     /locus_tag="PLM30_05295"
     CDS             complement(588..2594)
                     /locus_tag="PLM30_05295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOR54620.1"
                     /translation="MRRSFTRGISVLVLFFIVGLSAAFASGNPAAGYPETVRTARETL
                     WKEVTSGGVNSGSVAVMDKGKVVFSEEYGPADRAEGRMVDEKTRFNIGSTSKMFAAVA
                     VLILVDEGKIRLDDPVVKHIPEFRMRDPRYRYITIRMLFNHSSGLPGSTFVFGYRPEG
                     DPHETLLDALRESSLKHDPGAMGIYCNDGFTLAEMIVERVSEKKYIDFLKERVFGPLG
                     MDHTGESIGVTGGNVARFYDPDGKNYPLEVVTVLGAGGLSSTAEDLCRFADSFCPGGV
                     HILSPSSLEEIQGPQPTKFAPSLKGQALLEAFGWDHRLLPDFMELGYQILGKSGGTLF
                     YTTYLQLLPSERMAVAVIYSGSGNAGETAYKIMKAMMRDRGMPVPGEEPVDKPVKAEP
                     MAGYSFPSSGFLTNLSGFFRFNVDRKKGLLDIYPVAGAENSGAVSPALSLVYNGGLFH
                     NPEKRERYYLVSSDEGIFLVLSNVPRYGVDGPVFQLLEIPESPRKMSVEMDGTLWLVR
                     NSPTFSSFTDLLVLRSSAADELPGFFVFDGPKRVVDPDHAEMVSTGFRDQSSLRLFRK
                     DGRTRAKVLQFIYSMEEAAEPLAEGSTLAAIGADGENEWFKTVSGGILSFELTGKSRV
                     VVLPGPETPPLYDSVVDDGEVYVPEGSFIFLAGDPGDLIHISMR"
     gene            complement(2800..2895)
                     /locus_tag="PLM30_05300"
     CDS             complement(2800..2895)
                     /locus_tag="PLM30_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54621.1"
                     /translation="MKGAVRLLARKMGKSRYTLYADLRAFHSRRK"
BASE COUNT          618 a          901 c          808 g          585 t
ORIGIN      
        1 cgtcatgtcc tcctcctatc aataccgccc tcctcgccgc cctggccctg gcaaggcgcc
       61 gggcaacttc ggtggagagc aggtcgaggt cgatctccct gcctatccgt ttcaattcaa
      121 ggcggtcggc gtcccagagg caggccgcga gaccatcgtc ggtgacctcc ccatcggcat
      181 ggcgcgcgat ggcgtgacag atccgatcgg cgtccagatg tgggtagaac ctccccagca
      241 accgccgtgc aaggactgat gaatcgtggg cgtgaccgtc gcccgccttg tcatcggtcc
      301 tggcgcaatc gtgcaggaag cccatcacga cggcagcctc cacgtcctcc ccgaccgccg
      361 aggcaagccg tcccgaaagg atcgacaccc tcctcaagtg ggagatccca tgcagggcca
      421 gtccttcgca ggtacagaaa ggggccagct gaacctcaac ctctttgacc ataaggagta
      481 tctcttcttt tttcatcgcc cgttcctttt ttcattttcc ccgggccaaa aaaactcccg
      541 gtacttgccg ccgacccata cctttttacc cgacggagaa aaaagggtca acgcatagaa
      601 atatggatca ggtcccccgg atcgccagcc aggaagatga agcttccttc cggcacgtag
      661 acctcgccgt cgtcgacgac gctgtcgtac aagggaggcg tctcggggcc gggcagcacc
      721 accactctgc tcttcccggt cagctcgaag gagagtatcc ctcccgaaac ggtcttgaac
      781 cactcgttct cgccatcggc tcctatggcc gccagggtcg aaccttccgc gagcggttcg
      841 gccgcctctt ccattgagta gatgaactgg aggaccttcg ccctggtcct tccgtccttt
      901 cgaaacagcc tcaaggaaga ctggtcacgg aaacctgtcg agaccatctc ggcatgatcg
      961 gggtcgacca cccttttggg gccgtcaaag acgaaaaacc cgggaagttc atccgccgct
     1021 gaagacctca ggaccagtag atccgtaaag ctgctgaagg tgggggagtt cctgaccagc
     1081 caaagggtcc cgtccatctc gaccgacatt ttccgggggc tctccgggat ttcaagcaat
     1141 tgaaagaccg ggccgtctac gccgtaccgg gggacattcg acagcaccag gaagatcccc
     1201 tcatcggagg agaccaggta gtacctttcc ctcttctccg ggttgtggaa aagaccgccg
     1261 ttatagacca gtgaaagggc cggggacact gctccactgt tttccgctcc agcgacggga
     1321 taaatatcga ggaggccctt tttcctgtca acattgaaac ggaagaaacc cgataggttg
     1381 gtcaaaaaac ccgaagatgg aaagctgtaa cctgccatcg gctctgcttt gacgggttta
     1441 tctaccggtt cctctccagg tacgggcatc cccctgtccc gcatcatggc tttcatgatc
     1501 ttgtaagcgg tctccccggc gtttccggac cctgagtaga tcaccgccac ggccatcctc
     1561 tccgaaggga gaagttgcag gtatgtggtg taaaacagcg taccgccgct cttgcccagg
     1621 atctggtacc ccaactccat gaagtcagga agaaggcggt ggtcccagcc gaaggcctct
     1681 aaaagggcct gccccttcag agagggcgcg aacttcgtgg gctggggtcc ttgtatctct
     1741 tccagggatg agggagacag gatatgtacg cccccgggac agaaactgtc ggcaaaacgg
     1801 cagaggtcct cggctgtgga tgaaagtccc ccggcgccaa ggaccgtgac cacctcaagg
     1861 ggatagttct tcccgtccgg gtcgtagaac cttgccacgt tgcccccggt cacccctatg
     1921 ctctccccgg tatgatccat cccgaggggg ccgaacaccc tttccttgag gaaatcaatg
     1981 tacttttttt ctgagacccg ctcgactatc atctccgcaa gggtaaaacc gtcgttgcag
     2041 tatatcccca tggctccggg gtcatgcttc agtgagctct ccctcagggc gtcaaggagt
     2101 gtttcgtggg ggtccccctc gggtctgtac ccgaaaacga aggtggaccc cggaagccct
     2161 gaggagtggt tgaagagcat tcttatggtg atatacctgt aacgggggtc tctcatccga
     2221 aattctggga tgtgcttcac gacggggtcg tccaggcgga tcttgccctc atcgacgagg
     2281 ataaggaccg cgacggcggc gaacatcttg ctcgtggatc cgatgttgaa cctggtcttt
     2341 tcgtcgacca tcctaccctc cgccctgtcg gcgggaccgt actcctcgga gaacacaacc
     2401 ttgcccttgt ccatgacggc cacggacccc gaattcaccc cgcccgaagt gacctccttc
     2461 cagagggtct cccgagcggt ccttaccgtt tcagggtacc cggcggcggg attcccggag
     2521 gcaaaagccg cggaaagacc gacaataaag aataggacta ggacggagat cccccgggtg
     2581 aacgaccttc tcatgaaacc gacctcctct ctcgggccag gaccacggcg aaaacccgcg
     2641 catgatgacc cgtgggtttc ctgtagataa tttactccca aaaggacgat cccggggact
     2701 ggggatatca acgcgatccg gtcaagcgtt tcaggaagga gttgaaaggg aaggtcatcg
     2761 ccagggtcag tggagaacgg cgatcctgcg gccatcgagt cattttcgcc gtgaatggaa
     2821 agcccgcaga tccgcgtaaa gggtgtaacg gcttttaccc attttgcgcg ccagcaaccg
     2881 gaccgcgccc ttcaggttga aaaaaccgta cc
//
LOCUS       DAOSRG010000145         2896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_31151_length_2896_cov_8.994016, whole genome shotgun sequence.
ACCESSION   DAOSRG010000145 DAOSRG010000000
VERSION     DAOSRG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31151_length_2896_cov_8.994016"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..865
                     /gene="codY"
                     /locus_tag="PLM30_05305"
     CDS             32..865
                     /gene="codY"
                     /locus_tag="PLM30_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOR54622.1"
                     /translation="MDTPKELIESRAPIANPSAMQDLLEKTRAVNRILQSRKDPGTPD
                     YQRLARLLCELSAANVYIINREGVILGYSWVSEYNCPIMSDLLETGSMPESYTEKVNQ
                     YHESELNHTDHGLCAYSDEPCTYSNKHVVYVPIHGAGERLGTLILARFGYPFDNRDLV
                     LGEYLATVVGLEILHARARTIADRARERLVVQMAMRALSYSEVESIRQIIRELKGSEG
                     IVVASRVADRVGVTRSVIVNALRKLESAGIIESRSLGMKGTFIKVLSPLFLEDLGVTE
                     Q"
     gene            1009..1431
                     /locus_tag="PLM30_05310"
     CDS             1009..1431
                     /locus_tag="PLM30_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOR54623.1"
                     /translation="MTHEKGSQMTEVKVGDIVTGTVEQILPYGAFVRLSTGQKAMIHI
                     SELAHEYVKKVEDILRVQQEVRAKVLKIDERGRIDLSLKQLQARPERPRQQRGAPSAG
                     GDDFEKKLASFLKASDQKLSDLNKKSKEGSGGKPRGKK"
     gene            1464..2033
                     /locus_tag="PLM30_05315"
     CDS             1464..2033
                     /locus_tag="PLM30_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOR54624.1"
                     /translation="MLPPVFFEPPLPGDIDVVKRQAGSRRFDASLVRGVSRRCRYGFP
                     QVTLCDPLKKGRPFPTVFWLTCPHLSLRADELESAGEVRSLGRHIESENSEGWRVFNL
                     EYLLFRVFLLGPERTRNIMREDPSAWNSFAATGVGGIRAGGGVSMKCLHLQAASMIGF
                     GWHPGEGRLQEMLGEVECGDPSAWPCGAP"
     gene            1994..>2896
                     /locus_tag="PLM30_05320"
     CDS             1994..>2896
                     /locus_tag="PLM30_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOR54625.1"
                     /translation="MRRPFGLALWGAMRFGAIDLGSNSVRCLAVRARGEVLEYIDSGA
                     WITRLAEGIGGGTVSVGDKPLGRTLDAVGEALHLLGRSGVPLGHVVFRATESLRSATN
                     SDKIIGAMEELTGLHLEVLDPAEEARLGWEGASLGVPGAGMVFDLGGGSLDGGTADRT
                     FSFPLGAVRMTALFGEDPGAVMEETVRAVGNSLEGPPGRLVGVGGTSSSAVMMLEGVP
                     VTEYTPARLHGRTVKSEELKRLTARILATDPEDRSSITGLEKGRADIIVAGLSVIRAF
                     LGLAGALEYTHSETDLLWALCREAA"
BASE COUNT          577 a          828 c          999 g          492 t
ORIGIN      
        1 ccccacgtta ccgggaggga tcatcatgac aatggacaca ccgaaagaac tgatcgaatc
       61 gagggccccg atcgccaatc cttcggcgat gcaagacctg ctcgagaaga cccgggccgt
      121 caacaggatc ctccagagcc gcaaggaccc ggggacaccg gactaccaga ggctagccag
      181 gctcctctgc gaactgtcgg cggcgaacgt ctacatcatt aaccgggagg gcgtcatcct
      241 gggctactcc tgggtgagcg aatacaactg ccccatcatg tcggacctcc tggagacggg
      301 ttccatgccc gagagctaca ctgaaaaggt caaccagtac cacgagtcgg agctcaacca
      361 caccgaccac ggcctctgcg cctactccga cgagccctgt acctactcga acaagcacgt
      421 ggtctacgtc ccgatccacg gggccggtga acggctgggg acgctcatcc tggcccgttt
      481 cggctacccc ttcgacaacc gcgacctcgt gctgggggag tacctggcca ccgtggtggg
      541 tctcgagatc ctgcacgcca gggcgaggac catcgccgac agggcccggg aacggctggt
      601 ggtccagatg gccatgaggg ccctctccta ctcggaagtg gagtccatca ggcagatcat
      661 cagggaattg aagggctccg aagggatagt agtggcgagc cgcgtcgccg acagggtcgg
      721 ggtgacgagg agcgtcatag tcaacgccct gagaaaactg gagagcgccg gcattatcga
      781 gagccggagc ctcggcatga aggggacctt catcaaggtc ctgagccccc tcttcctgga
      841 ggacctcggg gtgacggaac agtagttgaa tgttccccgt cgccgccggt gacgaaggtt
      901 gcgtatcgcg gcgcacgaaa tgatatactt gacgacgcgg accgggcctg acggggccgg
      961 tgtattcata gatcattttt caaatattac ggaggggaat accggttgat gacccatgag
     1021 aaaggctccc agatgacgga ggtcaaggtc ggggatatcg tcacgggtac agtggaacag
     1081 atcctgccat acggggcctt tgtcaggctt tcgacggggc agaaggcgat gatccatatc
     1141 tcggaactgg cccacgagta cgtcaagaag gtggaggaca tcctgcgggt ccagcaggag
     1201 gtaagggcca aggtcttgaa gatcgacgaa aggggccgga tcgatctctc cctgaagcaa
     1261 ctccaggccc ggcccgagcg cccgaggcag cagaggggcg ccccgtcggc tggaggggat
     1321 gatttcgaga agaaactggc ctcattcctg aaggcgagcg atcagaaatt atccgatctg
     1381 aacaagaaga gcaaggaagg atccgggggg aaaccgcggg ggaaaaaata gggcggtggc
     1441 cgaaagcgcc tgtcacaggg ggcatgttgc cccctgtttt ttttgagccc cccctccccg
     1501 gcgatattga tgttgtgaag agacaggccg gttccaggcg tttcgacgcg agcctggtgc
     1561 ggggcgtttc gagaagatgc aggtacggct ttccccaggt gaccctctgc gaccctctaa
     1621 aaaagggccg gcccttcccg acggtcttct ggctgacctg ccctcacctc tctctaaggg
     1681 ccgatgaact ggagtccgcc ggagaggtga gatccctggg ccgccacata gagagtgaga
     1741 attccgaggg ttggagggtt ttcaacctgg aatacctgct tttcagggtt ttcctcctcg
     1801 ggccggagag gaccaggaat ataatgaggg aggacccctc agcctggaac tccttcgccg
     1861 ccacgggagt ggggggtata agggcgggag ggggcgtctc gatgaaatgc ctccacctcc
     1921 aggcggcctc catgatcggg ttcgggtggc accccggcga gggccggctc caagagatgc
     1981 tgggtgaggt cgaatgcggc gacccttcgg cctggccctg tggggcgcca tgaggttcgg
     2041 cgctatcgac ctgggttcca actcggtccg gtgcctggcg gtcagggctc gcggagaggt
     2101 gctcgagtac atcgactcgg gggcctggat aacgaggctg gccgagggca tcggcggagg
     2161 gaccgtttcg gtcggggaca agcccctggg gaggaccctc gatgccgtcg gcgaggcgtt
     2221 gcacctcctg ggaaggtcgg gagtccccct gggtcacgtc gtcttccgcg ccaccgagag
     2281 cctgaggagt gcgacgaaca gtgacaaaat aattggggcc atggaggagc tgacggggct
     2341 ccacctggag gtgctggacc ccgccgagga ggcccggctc gggtgggaag gggcgtcgct
     2401 gggggtcccc ggggcgggga tggtcttcga cctcggcggc ggcagtctgg atggggggac
     2461 cgccgatcgg accttttcct ttcccttggg cgccgtcagg atgaccgctc ttttcggcga
     2521 ggacccagga gcggtgatgg aagaaacggt ccgcgccgtc gggaacagcc tggaaggtcc
     2581 tccgggaaga cttgtcgggg tgggggggac ctcctcatcg gcggtgatga tgctggaagg
     2641 ggtccccgtg acggaataca cccccgcgcg gctccacggg cggaccgtga aaagcgaaga
     2701 acttaagagg ctgaccgcca ggatcctggc gacagatccc gaggaccggt cgtccatcac
     2761 cggcctcgag aaggggaggg ccgacatcat cgtggcggga ctttcggtga tcagggcctt
     2821 cctcgggctc gccggggccc tggagtacac ccatagcgag acggaccttt tatgggcgct
     2881 ttgccgggag gcggcg
//
LOCUS       DAOSRG010000146         2889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_31236_length_2889_cov_8.323924, whole genome shotgun sequence.
ACCESSION   DAOSRG010000146 DAOSRG010000000
VERSION     DAOSRG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31236_length_2889_cov_8.323924"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="PLM30_05325"
     CDS             complement(<1..104)
                     /locus_tag="PLM30_05325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOR54626.1"
                     /translation="MKKPWITTRGGDKGTTGLGDGSRLPKDHPRVELYG"
     gene            complement(178..612)
                     /locus_tag="PLM30_05330"
     CDS             complement(178..612)
                     /locus_tag="PLM30_05330"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOR54627.1"
                     /translation="MDVSTIRIRHYAPEGAQCGPVKETYDTLCNMVEQLGPKLARIGF
                     PVTLEKASSDGDPEKNNLVTLESADIDFPETPLDEILGYEASSKPCGCDGGDGHGRCR
                     TIEVAGQTYQAVPPGLIADGLLRVALSSTGGCGSGCGTCGGG"
     gene            complement(996..1427)
                     /locus_tag="PLM30_05335"
     CDS             complement(996..1427)
                     /locus_tag="PLM30_05335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR54628.1"
                     /translation="MVIRMALEELEGSKGCFVCDRSGANPRSLGVVLYWDGDSGRTVI
                     PFTPDDTWCGYEGIVHGGILAALCDDVMAWAARQALGEWTVTAGMSIRFLRPVKSGVG
                     YTAFGNALSVDGRKVKTAARIVGEDGKTCVDAKATFVGVRP"
     gene            1581..>2889
                     /locus_tag="PLM30_05340"
     CDS             1581..>2889
                     /locus_tag="PLM30_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOR54629.1"
                     /translation="MFRSCYSGRRLVVDLEKRSFGFEIIEPQEFRKYVGGRGLNAHYA
                     YSRKLHLADAFSDENELLIAPGALVGMPCLFANRTTLTSKSPLTGLLGDSNVGGDFGV
                     FLKWAEIDQVVIRGRSASPCFLVIKKNGCLFEDASDLWGRDVGETEEILRQRYGSSKR
                     SSTLTIGPGGENRVRFASIHSGAHAAGRTGLGAVMGDKGVKSIVVQGGVPRFDELGLD
                     KGFMKRLDSLVKNDPSYEGAKQYGATTAIEGFNKIGVLEVKNLQTGVWDQHEKISGKY
                     LRDTFTVRHYGCIFCNIQCKGISRFKNETDGEYEVHGPEFAAMQNYGARILNSDLPSV
                     CKMTKLSNNKGVDFMSMGAVLSWLMECLDRDFITIKDLDGVGLDWGKVDESIELIRKV
                     ATREGVGNILAEGGERASRSLGEKTRDCLLTVKGMEFEGCDPRG"
BASE COUNT          565 a          794 c          860 g          670 t
ORIGIN      
        1 ccgtaaagct ccaccctggg gtggtccttg gggaggcggg aaccgtcgcc gaggccggtg
       61 gtgcccttat cccctcccct ggtcgttatc cagggctttt tcatcaggat cgcctccgtc
      121 cgatgtaagg ttcaattaaa aaggggctgc cgccggcagc cccttgacct agatccctca
      181 gcctccgccg caggtgccgc agccgctccc gcagcccccg gtggacgaga gggccacccg
      241 gagcaggccg tcggcgatga gcccgggagg gacggcctgg taggtctggc cggccacctc
      301 gatggtccgg catcggccgt ggccatcccc cccgtcgcag ccgcagggct tggaggaagc
      361 ctcgtacccc agtatctcgt cgaggggggt ttcggggaaa tcgatatcgg cgctctcgag
      421 ggtcaccagg ttgttttttt ccgggtcccc gtcggaggag gccttctcaa gggtcacggg
      481 aaagccgatc cgggccagct tcggcccgag ttgctccacc atgttgcaga gggtgtcgta
      541 ggtctccttc acggggccgc actgggcccc ctcgggtgcg tagtgcctga tcctgatcgt
      601 tgaaacgtcc atatcacgaa cctcctatat tggcgtgtgc agtttttgtc cttcagttgc
      661 cttgattttt tgtcttgtct tccgtctttg ggtctttcct gtcgttggtt gccctggttt
      721 tttgtttttg ctcttacgat ttacgattca cggtttttgg tttccgattc ccgactcacg
      781 gcccctgccc ctcctgttgc cagcagcctg atgcctgtcg cctggttttc cgattcccga
      841 atcacgattt atgtatcttg cccttcctgt cccttggttt ttatgctcct acgtcttaca
      901 tctcacatct cacgtcttac gccccacgct ctcggatttt ctgtctcctg aaacccgacg
      961 cctgtcgcct tgttttcgta tacaattgct caaattcaag gtctgacccc aacgaaggtg
     1021 gccttggcgt cgacgcaggt ctttccgtct tcaccgacga tccgggcggc ggtcttcacc
     1081 ttcctgccgt ccaccgaaag ggcgttgccg aaggcggtgt acccgacgcc cgatttgacg
     1141 ggccgcagga agcggatgct catgcccgcc gttacggtcc attccccaag ggcctgcctg
     1201 gcggcccagg ccatgacgtc gtcgcagagc gcagccagta tgcccccgtg gacgatgccc
     1261 tcgtaaccgc accaggtatc gtccggggtg aagggtatca ccgtccggcc ggagtcaccg
     1321 tcccagtaga gtacaacacc gagggagcgg gggttcgcgc cgctgcggtc gcagacgaag
     1381 caccccttgg agccctccag ttcttccaag gccatcctta tcaccaccgg caggtatttt
     1441 accccaaggg gtatggagag tacaccccgt ttttcaaagg gtttttgcca ggtcggagcg
     1501 ttgacaatta aatcctagat tctatacttt ttcaaaagaa aacgatatat cgtatattgt
     1561 agactttggg gtgagtgtga atgttcagat cctgctacag tgggcgtcgt cttgtcgttg
     1621 accttgagaa gagatctttc ggattcgaga tcatcgaacc ccaggagttc aggaaatacg
     1681 ttggcggcag ggggctgaac gcccattacg cctactccag gaaactgcac ctggcggatg
     1741 ctttttccga tgagaacgaa ttactgatcg cgccgggggc attggtcggg atgccctgcc
     1801 tttttgctaa ccggacaacc ctcacttcaa agtcacccct gaccggtctg ctgggcgatt
     1861 caaatgtcgg cggagatttc ggcgttttcc tcaagtgggc cgagatagac caggtggtca
     1921 tccgtgggag atccgcttcc ccctgttttt tggttataaa aaagaatggc tgtcttttcg
     1981 aggatgcctc ggacctttgg ggtcgggacg tgggcgagac cgaagagatt ttacgtcagc
     2041 gctacggaag ctcaaagcgg tcctcgaccc ttaccatagg acccggcggg gagaacaggg
     2101 tgaggttcgc ctcgatccat tccggcgccc acgcggcggg aaggacaggg cttggggccg
     2161 taatgggcga taaaggggtc aagtccatcg tcgttcaggg aggggtcccg aggtttgatg
     2221 aactgggcct ggacaagggg ttcatgaagc gcctggattc cctggtcaag aatgatccga
     2281 gctacgaagg ggccaaacaa tacggggcta ccacggcgat cgagggtttt aacaagatag
     2341 gtgtcctgga agtaaagaac ctgcaaacgg gggtctggga ccagcacgaa aagatctcgg
     2401 ggaaatattt aagggatact tttacagtaa ggcactacgg atgcattttc tgtaatatcc
     2461 agtgcaaggg catttcgagg ttcaaaaatg aaaccgacgg cgagtatgaa gtccacgggc
     2521 cggaattcgc cgccatgcaa aactacgggg cccgcatcct gaacagcgac cttcccagcg
     2581 tatgtaaaat gacaaaactc agcaacaaca agggcgtcga cttcatgtcc atgggggcgg
     2641 tcctctcatg gctcatggaa tgcctcgaca gggattttat cacgatcaag gacctggacg
     2701 gggtcgggtt ggattggggc aaggttgatg aatcaattga actgatccgt aaagtggcta
     2761 cgagagaagg tgtgggtaat atcctggccg aggggggcga gcgggcgtcg agatctctgg
     2821 gagagaaaac gcgggattgc ttgttgacgg taaaagggat ggagttcgaa gggtgtgatc
     2881 cgcgcgggt
//
LOCUS       DAOSRG010000147         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_31329_length_2882_cov_8.400778, whole genome shotgun sequence.
ACCESSION   DAOSRG010000147 DAOSRG010000000
VERSION     DAOSRG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31329_length_2882_cov_8.400778"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /gene="sfsA"
                     /locus_tag="PLM30_05345"
     CDS             <1..1151
                     /gene="sfsA"
                     /locus_tag="PLM30_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOR54630.1"
                     /translation="RASRRPVIEARFLGRPNRFKVTAKIDGGSVEAYLPNPGRLWELL
                     LPDAKLFLENSGEVKDRKTDFTVIAVGTPHGPVMLHTHRTNDAAQWLLERGLVPGWED
                     AAIIRREVAFGGSRFDFLLEGRDGRFPVEVKSCTLFDGKMAMFPDTPSERATRHIAHL
                     AEIGRRGAGAGLLVLVHSPRPRYFLPNLHTDLDFAKAFLEARGVVDIKPLSVGWKEDL
                     TLETDGSLLEIPWPTLERNAVDKGGYILVLELGQDTRLPIGKLGVIDLKAGFYCYVGS
                     AMKNLKARMTRHRRERKKLHWQVDHLREASRFVACLPVRSAETVECDLARGLSRIADG
                     RIPGFGCSDCSCSTHLFRFETDPLKNPSFAGWLTEFRLDRLITGEGIP"
     gene            1148..1819
                     /locus_tag="PLM30_05350"
     CDS             1148..1819
                     /locus_tag="PLM30_05350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR54631.1"
                     /translation="MSVFDDHAKSYDGWYTTPIGKRVDEIETRCAFGLLEPRSGMKVL
                     DAGCGTGNFSLKLARLGCAVTGVDLSCEMLAIAIEKARAENLEVKFLPMDVKALEYVD
                     GAFDAVISMAALEFIENPLDAAEEMYRVVRRGGRLVIGTINRLSPWGELYMGIGRRSD
                     SVYRHARFLSLEEMSRFRPENLVSTRECLLFRPDNDVDNSRSGEDHGPAEPGKGGFLC
                     AMWKK"
     gene            1807..2058
                     /locus_tag="PLM30_05355"
     CDS             1807..2058
                     /locus_tag="PLM30_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HOR54632.1"
                     /translation="MEEMITCQVSFLPIESQNYKEDVTRVLSMIEKSGLDFQTGDMST
                     TLRGERDRIWALAQEIFEGMDPVCRFVLDLKVSNTCGCS"
     gene            complement(2035..2808)
                     /locus_tag="PLM30_05360"
     CDS             complement(2035..2808)
                     /locus_tag="PLM30_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOR54633.1"
                     /translation="MEYSMVFRVGHSEVGVHGRMRVVSVINRFQDIAGEHVDRLGFGV
                     ADLLRGRRTWVLHRMRLVFSRVPGLGEELKIRTWYRPERDLYSLRDFSMEDADGERVV
                     FAESSWVVLDLKRGRPVRLSSAMPRSYDENRAEYFSTGFKEIPESEDHDIAVRFDVRL
                     NDLDINRHVNNARYFEWALEAIPGEVLGSAGPSEVEALFRTPARYGEGIVSQVKKVSD
                     DPAVYLHRLRPEGSKETCAVLKTVWPVGTGPASGAAAGI"
BASE COUNT          604 a          880 c          863 g          535 t
ORIGIN      
        1 tcagggcttc cagacgcccg gtaatagaag cccgttttct tggcaggccc aaccgcttca
       61 aggtcacggc aaagatagac ggtggatcgg tcgaagccta tctccccaac ccagggcgcc
      121 tgtgggaatt gctcctcccc gacgcgaagc tcttcctcga gaattccgga gaggtaaaag
      181 accggaagac cgacttcacc gtcatcgccg tcgggacgcc ccatggcccg gtgatgcttc
      241 acacccatcg gaccaacgat gccgcccaat ggctcctgga acggggcttg gtgcccggtt
      301 gggaggatgc ggcgataatc cgccgcgaag tggccttcgg cgggagccgc ttcgatttcc
      361 tcctcgaggg ccgggacggg cgcttccccg tggaggtgaa atcctgcacg ctcttcgacg
      421 ggaagatggc catgtttccc gacacgcctt ccgagagggc gacccgccac atcgcccacc
      481 tcgccgaaat agggcgaaga ggtgcgggag cggggctcct ggtcctggtg cattcgcccc
      541 gtcctcggta cttcctgccc aacctgcaca ccgacctgga cttcgccaag gccttcctcg
      601 aagccagggg agtcgtggat atcaaacccc tgagcgtcgg ctggaaggaa gatctcaccc
      661 tcgagaccga cggttcactg ctggagatcc cctggcccac ccttgaacgg aacgccgtcg
      721 acaagggggg ctacattttg gtactggagc tcgggcagga cacccggctt ccgatcggaa
      781 agctcggggt gatcgatctc aaggcggggt tttactgcta cgtgggatcg gcgatgaaga
      841 acctgaaggc tagaatgacc cgtcatcgga gggaacgcaa aaaactccac tggcaggtgg
      901 atcaccttcg cgaggcctcc cggttcgtcg cctgcctccc ggtcaggtcc gccgagaccg
      961 tagagtgtga cctggcacgg ggcctctcaa ggatcgccga tgggaggatc cccggtttcg
     1021 gctgttccga ctgctcctgc tcgacgcacc ttttccgctt cgaaaccgat cccctgaaaa
     1081 acccgtcctt cgcgggatgg ctcacagaat tcaggttaga ccggctgata accggggagg
     1141 ggatcccatg agcgtttttg acgaccatgc gaagtcttac gatgggtggt acacgacacc
     1201 catcgggaag cgagtcgatg agatcgaaac caggtgcgct ttcgggctac tggaaccaag
     1261 aagcggcatg aaggtcctcg atgccggctg cggcacgggc aacttcagtt tgaaactggc
     1321 caggctggga tgcgcagtaa cgggtgtcga cctttcctgt gaaatgctcg ccatagccat
     1381 tgagaaagcc agggcggaaa acctcgaagt gaaattcctg cccatggatg taaaggccct
     1441 ggagtacgtt gatggggcct tcgatgccgt catctccatg gcagcgctgg agttcatcga
     1501 aaaccccctt gatgccgccg aggagatgta ccgggtcgtc agaaggggcg ggaggctggt
     1561 catcgggaca ataaaccgcc tcagcccctg gggggaactt tacatgggta tcggacgaag
     1621 gagcgacagc gtttaccgcc acgcccggtt cctgagcctg gaagaaatga gccggttcag
     1681 gccggagaac ctggtttcga cgagggaatg cctgctcttc agacccgaca acgacgtgga
     1741 caactcgcgc tcgggggaag atcacggccc cgccgaaccg ggaaaggggg ggttcctttg
     1801 cgcgatgtgg aagaaatgat cacctgccag gtctcctttt tgcccataga gagccaaaac
     1861 tacaaggaag acgtcaccag ggtcctgtcg atgatcgaaa agagcggcct tgattttcaa
     1921 acaggtgata tgtcgaccac cctcaggggg gagagggacc ggatatgggc cctggcccag
     1981 gagatcttcg aggggatgga tccagtctgc cggttcgttt tggacctgaa ggtctcaaat
     2041 acctgcggct gctcctgaag ccggaccggt ccctacgggc catactgttt tcaagaccgc
     2101 acaggtctct ttcgaccctt ccggccggag cctgtggagg taaacggcgg gatcatcgct
     2161 cacctttttg acctgggaga cgatcccttc gccgtaacgg gcgggcgtcc ggaaaagggc
     2221 ctccacctcg gacggcccgg ctgaacccag cacttccccg ggtatcgcct ccagggccca
     2281 ttcgaaatag cgggcgttgt tgacatggcg gttgatatcc aggtcattaa ggcggacatc
     2341 gaaccgcacc gctatatcat ggtcttccga ttcggggatc tctttgaacc ccgtggaaaa
     2401 atattccgcc ctgttctcgt cgtaggaccg gggcatggcc gagctcaacc tgacaggacg
     2461 ccccctcttg aggtcaagga cgacccatga tgattcggcg aaaacgaccc tctccccgtc
     2521 ggcatcctcc atggaaaaat ccctcagcga gtaaagatcg cgctccgggc ggtaccaggt
     2581 acggatcttg agttcttcgc ccaggcccgg cacccgggag aaaaccagcc gcatcctgtg
     2641 gaggacccat gtgcgcctcc cacgaagcaa gtcagcgacc ccaaacccca accggtcgac
     2701 gtgttctccc gctatgtcct ggaaacggtt gatgacgctg accaccctca tcctcccgtg
     2761 aacccccacc tcggagtgac ccaccctgaa aaccatgctg tattccaagg accatcaccc
     2821 ttcccaagtc agggcggaag gcgcgaaggt tcccacgctg gacacccccg tgacgcggga
     2881 ag
//
LOCUS       DAOSRG010000148         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_31739_length_2855_cov_7.136071, whole genome shotgun sequence.
ACCESSION   DAOSRG010000148 DAOSRG010000000
VERSION     DAOSRG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31739_length_2855_cov_7.136071"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLM30_05365"
     CDS             complement(<1..523)
                     /locus_tag="PLM30_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOR54634.1"
                     /translation="MKDGFPVLSETIEGRLQDMSSPYIRPGLSRIAALLSKMGHPERA
                     FPAVHLVGTNGKGSVSAMIESLFLEAGYSTCLYTSPHLTDITERLRFSGLPVDGGVLL
                     RSLDRVESALGGQGDPGSRPTYFEVLTAAAFDAIADRSPDVAIIEAGMGGRLDATNMV
                     KDVALTVITAIGFD"
     gene            complement(534..>2855)
                     /locus_tag="PLM30_05370"
     CDS             complement(534..>2855)
                     /locus_tag="PLM30_05370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOR54635.1"
                     /translation="WEWKKEYGDRIINQLKRLGASCDWERERFTMDEGLSRAVRAVFV
                     RLYKENLIYKGSYIVNWCPRCLTALSDLEVEHRDERGTLFYVRYPLVDGSGYVTVATT
                     RPETILGDVAVAVHPRDERHRGIAGRRVLVPVTDRIVPIIEDTMVDPGFGTGLVKITP
                     AHDPNDFLVGQRHGLETIQVIDEKGFMSPEAGPLFEGLDSAEARKKMVSLLKEKGLLE
                     RTEEHDHAIGHCYRCGTVIEPYLSEQWFVKMAPLAAPGIEAVRDGRIRIIPEQWVGTY
                     NNWMENIRDWCISRQLWWGHRIPAWYCDDCGHVTVSEVDPDRCECCGGGNIHQDEDVL
                     DTWFSSALWPFSTLGWPDDTEDLKTFYPTSLLVTGFDILFFWVARMIMMGLKFMGKEP
                     FSETYIHALVRDEQGQKMSKSKGNVIDPLEVIDKYGADSLRMTLASLTVQGRDIFLSP
                     ERIESNRLFMNKIWNASKLALANIDGVAPGSLPDERHLRIHDRWIIRRVNEVNVQVRS
                     DLEGYFYGEAAKALYQFVWSELCDWYLEMAKPALRGLEGDRRQETTRTVMALVFDSTL
                     RMLHPFIPFLTEELWDTLGFGEGALIEEKGWPGILPLGEALGPGTPDPMEVFQELVRS
                     IRNLRAEAGLHPRQEALEARVVLSEGAAGEAGEIISANADLLRLLCRVREVSLSEDQG
                     RRPLGTMMSVASFGEVSLFVDQNLDRGAEMARLSAELERARKDLSRSMGKLGDPSFLS
                     RAPGDIVEQERQRLSETEDKIRVIERNMRNLSE"
BASE COUNT          531 a          916 c          789 g          619 t
ORIGIN      
        1 ggtcaaaccc tatggcggtt atgactgtca gggcgacgtc cttgaccatg ttggtagcgt
       61 ccagccttcc tcccatcccg gcttcgatga tcgccacgtc cggggagcga tcggctatcg
      121 catcgaaggc ggcggccgtc agtacttcaa agtaggtggg cctggatccc gggtcaccct
      181 gccccccaag cgcagactcg acccggtcaa gggagcgaag cagcacgcct ccgtcgacgg
      241 ggaggccgga aaatcgcagt ctttccgtta tatccgtcag gtgcgggctg gtgtaaaggc
      301 aagtggaata accagcctcc aggaagaggg attcgatcat ggcggagacg gaacccttcc
      361 cgttcgtccc aaccaggtgg acggcgggga aagccctctc cgggtgtccc attttcgaaa
      421 gcagcgccgc tatacgggac agtcccggcc tgatataggg ggaggacata tcctggagtc
      481 gaccctcgat cgtctccgac aagactggga acccatcctt catggccggc ctgtcattcg
      541 gaaagatttc gcatgtttct ttcgatcacc ctgatcttgt cctccgtctc cgagagcctt
      601 tgcctctcct gttcgacgat atcgccaggc gccctcgaaa ggaaggaagg gtcgcccagt
      661 ttacccattg aacgtgagag gtccttgcgg gctctctcaa gttccgccga gagccgggcc
      721 atctccgctc ccctatcgag gttttggtcc acgaagaggg aaacctcccc aaaagaggct
      781 acggacatca tggttcctaa gggccttctc ccttggtctt cgctgaggct gacctcacga
      841 acacggcaaa ggagacgcag gagatcggcg ttggcgctga tgatctcgcc cgcctcgccg
      901 gcggcgcctt cggaaaggac cacccgcgcc tccagggctt cctgtcgcgg gtgaagcccg
      961 gcctcggccc tcaggttcct tatgctcctc actagttcct ggaagacctc cattggatcc
     1021 ggggtcccag ggccgagggc ttccccgagc ggaagaatac caggccatcc cttttcctcg
     1081 atcagggccc cttcgccgaa gccgagggtg tcccagagct cctcggtaag gaagggtatg
     1141 aaggggtgca gcatccttag agtactgtca aaaaccaggg ccatgacggt cctggtggtc
     1201 tcctgcctcc ggtcgccctc cagccccctc aaggcgggct tggccatctc gaggtaccag
     1261 tcgcagagtt cactccagac gaactggtat agggccttcg ccgcctcgcc gtagaagtaa
     1321 ccctccaggt cacttctaac ctggacgttc acctcgttca cccttcggat tatccaccgg
     1381 tcatggatcc ttaagtgcct ctcatccgga agggagcccg gcgccacccc atcgatattg
     1441 gcgagggcca gtttactcgc gttccagatc ttgttcatga agagcctgtt gctttcgatc
     1501 ctttcggggc tgaggaaaat gtcccttccc tgcacggtga ggctggccag ggtcatcctg
     1561 agggaatcgg cgccgtactt gtcgatcacc tccaggggat cgatcacgtt gcccttggac
     1621 ttgctcatct tctgaccctg ctcgtccctt acgagggcgt gaatataggt ctcgctgaag
     1681 ggttccttgc ccatgaactt gagccccatc attatcatcc gggcgaccca gaagaaaagg
     1741 atgtcaaacc ccgttacgag caggctcgtg gggtaaaagg ttttcaggtc ctccgtgtcg
     1801 tcgggccagc ccagggtcga gaagggccag agggcgctgc tgaaccaggt gtcgaggaca
     1861 tcttcatcct ggtggatatt gccgccgccg cagcactcgc accggtcggg gtctacttct
     1921 gaaacggtga catggccgca atcgtcacag taccaggcgg gtatcctgtg gccccaccac
     1981 aactgcctcg atatgcacca gtccctgata ttttccatcc agttattgta tgtccccacc
     2041 cattgttcgg ggattattct tatcctgcca tcccgaaccg cttctatccc cggggcggcg
     2101 agaggagcca tctttacgaa ccactgctcc gagaggtagg gctcgatcac cgtcccgcat
     2161 cgatagcagt gcccgatcgc gtgatcgtgt tcctccgtcc tctcgagaag gcccttttcc
     2221 ttcaacaggg agaccatctt tttcctggcc tcggcactat ccaacccctc gaagagcggc
     2281 cctgcctcgg ggctcatgaa gcccttttcg tctatcacct ggatcgtttc aaggccgtgc
     2341 ctctgcccta cgagaaagtc gttcgggtca tgggccggcg tgatcttgac gaggcccgtg
     2401 ccgaaacccg ggtcgaccat cgtatcctct atgatgggca cgatcctgtc cgtcacgggg
     2461 accagcactc tcctccccgc gatccctcta tgcctttcgt cgcgggggtg gaccgccacc
     2521 gccacgtcgc cgaggatcgt ctcggggcgc gtggtggcga ctgttacata cccgctaccg
     2581 tcaacgaggg gataccgcac gtaaaaaagg gtgccccttt catcacggtg ttcgacctcc
     2641 aggtccgaca acgccgtcag gcaccgggga caccagttca ctatgtagct ccccttgtag
     2701 atgaggtttt ccttgtaaag ccttacgaaa acggccctga ccgcccttga cagcccctcg
     2761 tccatcgtga agcgctccct ttcccaatcg caggaagccc ccaggcgttt cagctggttt
     2821 atgatccggt cgccgtattc tttcttccat tccca
//
LOCUS       DAOSRG010000149         2850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_31820_length_2850_cov_10.625403, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000149 DAOSRG010000000
VERSION     DAOSRG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31820_length_2850_cov_10.625403"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..>1329)
                     /locus_tag="PLM30_05375"
     CDS             complement(268..>1329)
                     /locus_tag="PLM30_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOR54636.1"
                     /translation="LATWVMSLFIFIDDFLISLTLGTCMTPSADRLKIPKEMTAYVIN
                     SSAAPVASLVPLSAWAVFIAALLEKQLGLPAGEGLALFIKTIPFNFYAWFAFFMVPLV
                     VAGVVPVFGPMKRAEERARTTGVLAPPGSDLIAMRKPSSGGVEVPEGKARNLVLPLLV
                     LIAATMAFGVNLYKGAFTTLFFCAVYYRLQGVMTVPRFTGACLEGIGNMVFPIVFTCL
                     AFTFNQVNSELGLIPYIIGKAAPLLSPALFPLVIFVILGLVAFLVGISWSLYLIAFPL
                     VIPLAQALGADLVLAVAATVAAGTLGQHCCFYSDGTILVSAATGCDNISHALSQLPFA
                     MIAAGLACVAFLAAGVLSV"
     assembly_gap    1330..1429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1658..>2850
                     /locus_tag="PLM30_05380"
     CDS             1658..>2850
                     /locus_tag="PLM30_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOR54637.1"
                     /translation="MTVLHGTPEMAPLSKVGGLADVTGSLPGALRRLGVDARVITPAW
                     PGVAEKAGDLGARPLLGPEEVCVSLGGRRVCSRLRAVEVGGVPVYLLEEPDLFSNPAV
                     YPENLDAVTALPFAFLSLAAVELALSGEWKPMVLHLHDWTTSLAAAALKYHRLYGASP
                     GKPRTVLTIHNLAHQGVLPLSVLDDWGFGPGAFSLEGVEFYGRANLLKGGITSSDAIA
                     TVSPGYSREILTGEFGEGLEQVLRYRSDKVTGILNGLDTESWDPWRDPALPAHYRPGE
                     MRGKKICRKVLLKTCGWEDSGGPVIVSVGRLVRQKGMELVLGALDVLADLGARLVVVG
                     TGERALEERFSAEARQKPGVLHFRGAFDDAFARLAYAGGDLFVMPSLFEPCGLSQMIA
                     MRYGTV"
BASE COUNT          512 a          884 c          913 g          441 t
ORIGIN      
        1 ccctcgtagc gcttcagcag cggcgaatcc ttgctgcctt cgacgtcggc gccgaggcac
       61 agcgccggcg ccagggtcaa aaggagaaga ccgaacaaaa gcctgaaggc ccggatcctg
      121 ctaatggcgt gcatcgttac ccctccctcg gcggtccatt tttattttta tcttgatgcc
      181 ttgtcatcat tatagattgg cgccccaaag gcggcaagaa cgccgcttcc ccgggggggt
      241 ccggcccgaa tcgctgcccc cgtcctgtca gaccgacagg acccccgcgg ccaggaaggc
      301 cacgcaggcc agccccgccg ctatcatcgc gaagggcagc tggctcaggg cgtggctgat
      361 gttatcgcac cccgtcgcgg ccgacaccag gatggtgccg tcggaataaa agcagcagtg
      421 ctggcccaag gtccccgccg cgacggtggc cgccaccgcc aggaccaggt cggcccccag
      481 ggcctgcgcc aggggtatca ccagggggaa cgcaatgagg tacaggctcc agcttatgcc
      541 caccaggaac gccacgaggc ccaggatcac gaagatgacc agggggaaca gggccggcga
      601 gagcaggggg gcggccttgc cgatgatgta gggtatgagc cccagttccg agttgacctg
      661 gttgaaggtg aaggccaggc aggtgaacac gatggggaac accatgttcc ctatcccctc
      721 caggcaggcc ccggtgaaac gcggcacggt catgacgccc tggaggcggt agtagacggc
      781 gcaaaagaaa agggtggtga aggccccctt gtaaaggtta acgccgaagg ccatggtggc
      841 cgctatcagc accaggaggg gcaggaccag gttacgggcc ttaccctcgg gcacctcgac
      901 cccgccgctt gaaggcttcc tcatggcgat caggtccgat ccaggcggcg ctagcacgcc
      961 ggtggtccta gcccgctcct cggcgcgctt catggggccg aagacaggga cgacgccagc
     1021 caccaccagg ggcaccatga aaaaggcgaa ccaggcgtag aagttgaagg gtatggtctt
     1081 gataaaaagc gccaggccct cgccagccgg caggcccagt tgtttctcca gcagggcggc
     1141 tatgaagacg gcccaggcgg atagcgggac cagcgaggcc actggggcgg cggaggagtt
     1201 tatcacgtag gccgtcatct ccttggggat cttgaggcgg tccgccgacg gcgtcatgca
     1261 ggtccccagg gtgaggctga tcaggaagtc gtcgatgaag atgaagaggc tcatgaccca
     1321 ggtggccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcctggtcac
     1441 gaggacggcg gtcagaagga ccgtgatcgg cagcagggag acccaacctc cgggattcac
     1501 atcggtaacc tccatggggg gacaagagtt cttaccacca aaggatcata caccatgcca
     1561 tcggtggcga gccttcattc accatagtac aatagcatcg cagggtaaac acgattgggc
     1621 gtgagagaca ccgatgggga ctgatgatcc ttccggcgtg accgttctcc atgggacacc
     1681 cgaaatggcg cccctatcca aggtcggcgg gctggccgac gtgaccggtt ccctgccggg
     1741 cgccctgcgg cggctcggcg tcgatgcccg ggtgatcaca ccggcgtggc ccggcgtggc
     1801 tgaaaaggcc ggggacctgg gagcccgccc cctcctcggg cccgaagagg tatgcgtctc
     1861 cctcggcggc cgccgggtct gttcccgcct ccgggccgtc gaagtgggag gcgtgccagt
     1921 gtacctgctc gaggagccgg acctcttttc aaaccccgcc gtctacccgg aaaatctcga
     1981 cgcggtcacc gccctgccct tcgccttcct ctccctggcc gccgtggagc tggccctttc
     2041 gggggagtgg aagccgatgg tgctccacct tcacgactgg accacctccc tggccgcagc
     2101 ggcgctcaag taccaccgcc tttacggcgc ctccccgggg aagcccagga cggtgctgac
     2161 gatccacaac ctcgcccacc agggcgtcct acccctctcc gtgctggacg actggggttt
     2221 cggccccggc gccttctccc tcgagggcgt cgagttctac ggcagggcca acctgctcaa
     2281 gggaggcata acatcttccg acgccatcgc gaccgtcagc cccggttact cccgagagat
     2341 cctcactggc gaatttggcg aggggctgga acaggtgctc cggtaccgca gcgacaaggt
     2401 cacggggata ctcaacggcc tggatacgga gtcctgggac ccctggaggg acccggccct
     2461 gccggcgcat taccggcccg gcgagatgag gggcaagaag atctgccgga aggtcctgtt
     2521 gaagacgtgc ggctgggagg actccggtgg accggtgatc gtctcggtgg gcaggctcgt
     2581 ccggcagaag ggcatggaac tggtcctggg cgccttggac gtactggccg acctgggcgc
     2641 caggctggtg gtggtgggca ccggagagag ggccctggaa gaaagatttt ccgctgaggc
     2701 ccgtcagaaa ccgggagtcc tccatttccg cggggccttt gacgacgcct tcgcccggct
     2761 ggcctacgcc gggggcgacc ttttcgtcat gccgtccctc ttcgagccct gcgggctctc
     2821 ccagatgatc gccatgaggt acggcaccgt
//
LOCUS       DAOSRG010000150         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_32302_length_2818_cov_9.307637, whole genome shotgun sequence.
ACCESSION   DAOSRG010000150 DAOSRG010000000
VERSION     DAOSRG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32302_length_2818_cov_9.307637"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLM30_05385"
     CDS             complement(<1..542)
                     /locus_tag="PLM30_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54638.1"
                     /translation="MKRKSLIAVLLVVLVMSLASVATAEEKFHWRLQSSWATGLEIQR
                     QVERFAELVTKMSGGRLTITALPAGAVVGGLEVFDAVSQGVIDAMNSLSAYWIGKEPA
                     APMFSAIPLGMDVMQYYAWYYYGDGLDLMQEMYAKHNIGYAAFCAINPAEDFLWAHKP
                     ITKLSDFKGLKIRTVGYWGE"
     gene            complement(593..991)
                     /locus_tag="PLM30_05390"
     CDS             complement(593..991)
                     /locus_tag="PLM30_05390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR54639.1"
                     /translation="MEKKVIPFGSAMGMKVAFSTAVSVDVRSVDKIIWVSGQVSFDEK
                     GNILGKDDMYVQTEHVILNIQSALAEFGAGLDDVVEVNVFVRDMSRLKEIHEARLKYF
                     KEPFPTSTLVEVNKFVHPDALIEINAVAVI"
     gene            complement(1147..2709)
                     /locus_tag="PLM30_05395"
     CDS             complement(1147..2709)
                     /locus_tag="PLM30_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOR54640.1"
                     /translation="MRIACVQMEAPELADFERTFSRIQCLLGEALQPRVDLVVFPECA
                     WPAYFIDPDERRLLREALSLNERLIDHIRRKAREHASYVAFGLVEEKDGKLGNNAILV
                     DPSGEMVLRVSKMNLWHFDRDLYEPGQSFDVVDSPFGRIGLLICADARLPEIARVLSV
                     MGADLIIDPANLTGYSFSSKGLTNPQLDFMLPARAFENGVWWVLSNKCGIESAAVPYV
                     GGSRVISPEGVSVASASPDREEILFVDIGLPGRRPASLPERAPAAYGVLAEPTESLPV
                     TGITSQPAIPSCSSFFLGAAQFGAEDAEEYILKAGKHLATLGRLHASVAALPPADFLP
                     APGIHELADAIRKKHGAVMGGMIVALAGGGRGGEPLGVIFDAGKILASSRGRPPFSGS
                     DVIGTPHGNFAFVHGEGILLPEYARSLTLLGADLLFSLTDSLSGPSLEIARTRAYENK
                     VFLVRSASPGEGKRSFICDPYGRILSSAFIGGEQVFGCQVELLSARCKEVVPGTDVIR
                     GRRPSDYRLLCR"
BASE COUNT          563 a          882 c          809 g          564 t
ORIGIN      
        1 atctcgcccc agtaaccgac ggtacggatc ttcaggccct tgaaatcgct gagctttgtg
       61 atcggcttgt gggcccagag gaagtcctcg gccgggttga tcgcgcagaa agcagcgtaa
      121 ccgatgttgt gcttggcgta catctcctgc atgaggtcga ggccgtcgcc gtagtaatac
      181 caggcgtaat actgcatgac gtccatcccg agcgggatcg ccgagaacat gggcgccgca
      241 ggctctttcc cgatccagta cgcggaaagg ctgttcatcg cgtcaatgac accctggctc
      301 acggcgtcga aaacctcgag ccctcccacc accgcgcccg ccggaagggc agtaatggtc
      361 aaccgtcccc cactcatttt cgtcacgagt tcggcaaatc tctcgacctg cctctggatc
      421 tccagtcccg tcgcccaact ggactgcagt ctccagtgga acttctcctc ggccgtggcg
      481 accgaggcca ggctcatcac cagtacaacc agcagaaccg ctatcaagct cttccttttc
      541 atctcttctc ccccttacga tgtcagatat cctgcgtttc ccggaccggt ttctagatca
      601 ctgccacggc attgatctct atcagggcgt caggatgcac gaatttgttg acttcaacca
      661 gggtgctggt ggggaaaggt tccttgaagt atttgagcct cgcttcgtgg atctccttga
      721 gccggctcat atctcttaca aacacattga cctcgaccac atcatccagg ccggccccga
      781 actccgccag ggcgctttga atgtttagga tgacgtgttc cgtctggacg tacatgtcgt
      841 ccttacccag gatgttccct ttttcgtcga aagagacctg gccggagacc cagatgatct
      901 tatcgacgga cctgacatcc acgcttacag cggtactgaa ggctaccttc attcccatgg
      961 cgctgccgaa cggtatgacc tttttctcca tacaagcacc tcctgagaat aatgggtggc
     1021 gaggtccagt tcctatgttc tatagaatat agactatata aatcttgtca agacggcaat
     1081 gcacttatag aggaacaccc cggtaaattg ggaaagaccc gccgcctggg gcctcgaagg
     1141 gccggcttac cggcagagca gccggtaatc ggaaggtctc ctgccccgga tcacgtccgt
     1201 acccgggacg acctccttgc acctcgccga caggagctcg acctggcagc cgaagacctg
     1261 ttcccctccg atgaaggcgc tggagaggat cctgccgtag gggtcacaga tgaaagacct
     1321 cttgccttcg ccgggggagg cggagcgcac caggaaaacc ttgttctcgt aggccctcgt
     1381 cctggctatc tcaagggaag gccccgacag ggagtccgtc agggaaaaca gcaaatccgc
     1441 gcccaggaga gtcaggctcc tggcgtattc ggggaggagg atcccctcgc cgtggacgaa
     1501 ggcgaagtta ccgtggggcg tcccgatgac atccgatccc gagaagggcg gacggcccct
     1561 ggaggacgcc aggatctttc ccgcgtcgaa gatcacaccg agaggttcac cgcccctccc
     1621 accaccggcg agagcgacga tcatcccccc cattaccgcc ccgtgctttt tccttattgc
     1681 atcggccaac tcgtggatgc cgggagcggg aagaaagtcc gccgggggga gggccgcgac
     1741 ggacgcatgc agccgtccca gggtggccag gtgcttcccc gctttgagga tgtactcttc
     1801 ggcgtcctcc gctccgaact gggcggcacc caggaaaaag ctgctgcagg aggggatggc
     1861 gggttgggaa gtgatccccg tcaccgggag gctctcggtg ggctcggcga gcaccccata
     1921 cgccgctggt gccctttccg ggagagacgc gggccttctc cccggcaggc cgatatcgac
     1981 gaagaggatc tcttccctgt cggggcttgc cgaggcgacg gagaccccct ccggggatat
     2041 taccctgctg ccgcccacgt aggggaccgc cgccgactcg atcccgcact tgttggagag
     2101 gacccaccat accccgttct cgaaagccct ggcgggcagc atgaaatcca gctgcgggtt
     2161 ggtcaggccc ttggaggaga aggaatagcc cgtgaggttc gccgggtcga tgatgaggtc
     2221 ggcccccatt acggagagca cccgggcgat ttccggcagc ctcgcgtcag cgcatatcaa
     2281 aaggccgatc cgcccgaagg gtgaatcgac cacgtcaaaa ctttggcccg gctcgtagag
     2341 gtctctgtcg aaatgccaaa ggttcatctt cgacactctc aggaccattt cccccgaagg
     2401 gtccacgagg atggcgttgt tgccgagctt cccgtccttt tcctccacca gtccgaaagc
     2461 cacgtaggaa gcatgttccc gggctttcct gcggatatgg tcgatcaacc gctcgttgag
     2521 cgatagggcc tccctgagga gacgcctttc gtcggggtcg atgaagtagg ccggccaggc
     2581 gcattccggg aacaccacca ggtcgacccg gggctggagg gcctcaccca ggaggcattg
     2641 gatcctgctg aaagttcttt caaaatcggc caattccggt gcttccatct ggacacaggc
     2701 aatcctcatt ttctaccccc cgcttcgctt ttttcccgta aaggaccggg gcaactgaga
     2761 cctccgaaag gcctatttgg ggcatcatgg tagaggctgc cggggcatcc gtcaaaca
//
LOCUS       DAOSRG010000151         2813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_32386_length_2813_cov_6.908267, whole genome shotgun sequence.
ACCESSION   DAOSRG010000151 DAOSRG010000000
VERSION     DAOSRG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32386_length_2813_cov_6.908267"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLM30_05400"
     CDS             <1..1454
                     /locus_tag="PLM30_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOR54641.1"
                     /translation="DPLLEKRLIEGCLEIHSKGLIVAMQDMGAAGIVSSSSEIASKSS
                     TGILVNVDLVPLKERMEPWETALSETQERMLLIVKEEDLGEIGSIADRWKLDWAVIGE
                     ITGDGNFVMTREGRTVVNLPAGMVGGGSPPIHWPSKEPSAEKTLEAKSKGSLDPEEVS
                     RSLAAILEDPNLADKSGIFGRFDSGAQSNTVTPPGKSVSIFRVPENGNLVVVTMEADP
                     WKCQLEPFQGTAETFLKCLRPLWVSGAVHLGMTNCLNFPSPEDPENFWVLERSVLGLA
                     AVGGDLACPVVSGNVSLYNESPDGKILASPLVAVVGLLEEGRAPLGDAAELETGSVFL
                     VGEEQGFLGGSAYSRIVDQAGTFPAGPYYPVKERTFMERAHRTAREGAAAAGRALAGG
                     GLLAALAKICQAGGIGISLDAGNLDLSTDRLFGEWGARAVYLVPEGSTDLFLSCWEGF
                     PVRRIGKITGGVLTLGEREIYPGRTAEEKVEGA"
     gene            1459..>2813
                     /gene="purF"
                     /locus_tag="PLM30_05405"
     CDS             1459..>2813
                     /gene="purF"
                     /locus_tag="PLM30_05405"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR54642.1"
                     /translation="MAGVFGAFCKNGHSVLEDVYLGLYALQHRGQESAGIAWNSNEGG
                     EVNFVKGMGLVHIALDQKYLAGLRANSAIGHVRYSAAGPSGLANVHPITANYSMGSVA
                     VAHNGNITNAMGIRHMLESRGAIFQSTTDTETIIHLMAHQSHKPPLDALVDALRRLRG
                     AYSLAVLLGNRLVAARDPWGFRPLVLGKREDTFYVASESCALDIVGASLVRDVKPGEI
                     IVIDEAGIKSIRIPVEASRLYSCAFEYVYFARPDSVVDGRSVYEVRKTLGCKLSASAP
                     CPTADIVTGMPDSGTIAAIGYAGCAGQRYEAAIVRNRFVGRTFIQPTQRVRALGVKIK
                     LNPIKKLVAGKELAVVDDSIVRGTTAERAISIIRQDGAARVHIRIASPPVRFPCYYGI
                     DMLSCEELAAAKLDIDTLRTTVGADSLYYLGIEDLVSSIGLPPSDICTACFSGEYLDG
                     G"
BASE COUNT          564 a          844 c          883 g          522 t
ORIGIN      
        1 gggatcccct cctggaaaaa agactgatcg aaggctgcct cgagatccat tcgaaaggcc
       61 tgatagtcgc catgcaggac atgggagccg cggggatcgt ttcatcctca agcgaaatag
      121 cctctaaaag cagcaccggg atactggtta acgtcgacct ggttcccctc aaggaaagga
      181 tggagccctg ggagacagcc ctttcggaaa cccaggaaag gatgctcctc atcgtaaagg
      241 aggaggatct gggtgagatc ggctccatag ccgacaggtg gaagctggat tgggcggtga
      301 ttggcgagat caccggcgac ggcaacttcg tcatgacccg ggaggggagg accgtggtca
      361 accttccggc ggggatggtc gggggcggct ctcccccgat ccactggcct tcaaaagagc
      421 cctccgcgga gaagaccctt gaggccaaaa gcaagggttc cctcgatccg gaagaggtct
      481 cgcgctccct tgctgccatc cttgaagatc cgaacctggc tgacaaatcc ggtattttcg
      541 gccgtttcga ctccggggcc cagtccaaca ccgtcacccc tcccgggaaa agcgtatcca
      601 tcttccgagt cccggagaac gggaatctcg tggtggtgac catggaggcg gacccctgga
      661 aatgtcaact tgagcccttc caagggacgg ccgagacctt cctcaagtgc ctgagacccc
      721 tttgggtgag cggtgccgtc cacctgggca tgacgaactg cctgaacttc ccgtctccgg
      781 aagacccgga gaacttctgg gtgctggagc ggtctgtcct gggcctggca gcggtgggcg
      841 gtgacctcgc ctgccccgtt gtttcgggta atgtcagcct ctacaatgaa agccctgacg
      901 ggaagatcct tgccagccct cttgtcgccg ttgtggggct cctagaagag ggaagggccc
      961 ccctgggtga tgccgctgaa cttgaaaccg gatcggtctt cctggtggga gaagaacaag
     1021 gatttttagg tggaagcgcc tactcgagga tcgttgacca agccggcacc tttccggcgg
     1081 gaccctatta ccccgtcaag gaaaggacct tcatggagag ggcccaccgt accgcccgcg
     1141 aaggggccgc cgcggcgggt cgagcgctgg ccggcggtgg gctgctggcc gccctcgcga
     1201 agatctgcca ggccgggggg atcgggatca gcctcgatgc cgggaacctc gacctttcca
     1261 ccgaccgcct cttcggagaa tggggcgcga gggcggttta cctcgtcccg gaaggatcga
     1321 ccgacctttt cctttcctgc tgggagggtt tccccgtccg gaggatcggt aagataaccg
     1381 gcggcgttct cactctcggg gaacgggaaa tatacccggg aaggacagcc gaagaaaagg
     1441 tcgagggggc ctagagccat ggctggggtt ttcggtgcct tttgcaagaa cggccattcc
     1501 gtgttggagg atgtctacct ggggctttac gccctgcagc accggggaca ggaatcagcg
     1561 ggcattgcct ggaactccaa cgaaggaggc gaagtcaact tcgtaaaggg gatggggctg
     1621 gtccatatag ccctcgatca gaaatacctg gccggcctga gggcaaactc cgccatcggg
     1681 catgtcaggt actctgccgc cggcccttcc ggcctggcca acgtacaccc gatcacggcc
     1741 aactattcca tgggctctgt ggccgtcgcc cataacggca acatcaccaa cgccatgggg
     1801 ataaggcaca tgcttgagtc gaggggcgcc atattccagt cgaccaccga cacggagacc
     1861 atcatccacc tgatggcgca ccagtcgcac aaaccgcccc tggacgcgct ggtggacgcc
     1921 ctcaggcgcc taaggggagc ttacagcctc gcggtgctgc tggggaaccg gctcgtcgcg
     1981 gcccgcgacc cctggggttt tcgacccctc gtcctgggga agcgggaaga caccttttac
     2041 gtggcctccg agagctgcgc ccttgacata gtgggcgctt cgctggtccg ggatgtaaaa
     2101 cccggtgaga tcatcgtaat tgatgaggcc gggatcaaga gcatcaggat ccccgtcgag
     2161 gcttcccggc tttactcctg cgccttcgag tacgtctatt tcgccaggcc tgacagcgtg
     2221 gtcgacggga ggtccgtcta cgaagtgaga aaaaccctgg gttgcaaatt atcggccagc
     2281 gctccctgcc ccaccgcgga catcgtcacg ggcatgcccg acagcgggac catcgcggcg
     2341 atcggttatg ccggttgtgc cggtcagcgt tacgaggcgg ccatcgtgag gaacaggttc
     2401 gtcgggagga cttttatcca gcccacccag agagtcaggg ccctcggagt gaagatcaag
     2461 ctcaacccga tcaagaagct cgtcgccggc aaggaactgg ccgtcgtcga tgattcgatc
     2521 gtgaggggca ccacagcgga acgggccatt tcaataatca ggcaggacgg ggccgccagg
     2581 gtccatataa ggatcgcctc cccgcccgtc cgtttcccct gttactatgg catagacatg
     2641 ctttcctgcg aagaattggc ggccgccaaa ctggatatcg ataccctgcg cacgacggta
     2701 ggggctgact cgctgtacta cctgggcatc gaagaccttg tctcatcgat cggcctcccg
     2761 ccgtcggata tctgcaccgc ctgcttcagc ggggaatacc ttgacggagg aga
//
LOCUS       DAOSRG010000152         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_32522_length_2804_cov_11.821753, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000152 DAOSRG010000000
VERSION     DAOSRG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32522_length_2804_cov_11.821753"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>2804)
                     /locus_tag="PLM30_05410"
     CDS             complement(115..>2804)
                     /locus_tag="PLM30_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR54643.1"
                     /translation="DEPVVDKMDINAQPFMMVVAKTALPSYQARDIVEDQVAKRLTQI
                     IGMASVDISGGRVREIHLDADPSSLIAHNLSLRRLASLVKASNFNSPSGNITLGGKET
                     SVRIVGEPDQSSKFEDLGIPVGDGRVIKLGNVVTVVDALADERNRARFDGRDSILLEL
                     IARPNANIVKVSGEVRAELDKIAPTLGKSLELEVVYDSSEFVEKSVRNVIRDMIIGTL
                     LTALFLYLFLRQFGSTLAVAIAMPTSIIATFIPMYFYKYTLNVMSTMGLAISVGVLVN
                     NAILVLENIYRYRELGMDPLTAAEEGTREISLAVLSTTATNLGVFIPIAFMGGIVGQF
                     FNQFALTVVFSTLFSLWGAFTVTPMAAARLGGSVHVSPFAKKVTAWWYWIYRELEVFQ
                     EVYVGKAMRHPWVTIGFFGLLFAGSLLLIPRVGFELFPRVDEGVIRINLELSSTASLE
                     ATDEQVRKVEAFLREQPYVKSMSSTVGGRRLSSVNSGVLRVYLVDADERKSAFAIASD
                     LRKKFAGLPDTNVTINVASGQGGGSRKPVQITISGPDLDVLNGIAAELMEKIRDIPGL
                     ADLDTDWKVGRFDLNLIPEDLKLTSMGVTLSDVSDELRGYLSGIKAGVYREQGYEYDI
                     LVKLASRWVDSPEAVPLLPMWTPHGFVPVNELAAVAYEPSPTAIYRLDRQRKISVQAD
                     VAGRTVGEVFRDITPRMDEIQLPTGYRFIIGGEIQNIQENFRRMVIAFAMAVVLTFLM
                     IAGILESYLFSLAIMLTIPLSVIGVIPTLLLTGTSLSMYGLLGLVMIVGLVVNNAIVI
                     VDYAEVVRKKGHEAAGAMIEACRVRFRPIIMADVTTLIAMIPLALGLGEGGQYRAPLA
                     IVLIGGLLAGGTMALFLIPPVYKVIWDIKNRFQGRDENA"
BASE COUNT          570 a          838 c          826 g          570 t
ORIGIN      
        1 atggcttccg ccggaacggg gactctttcc ggcacgggga ccctcccgaa gaaatagacc
       61 gccccggcga tgacgccaac agcgacgagt atggagaacc agaaggataa aaccctacgc
      121 attttcatcc ctcccctgga atcggttctt gatgtcccaa atgaccttgt agacgggagg
      181 tatgaggaac agggccatgg tacctcccgc cagcaggccc cctatcagca cgatggccag
      241 gggggccctg tactgcccac cctcaccgag gccaagggcg agggggatca tggcgatcag
      301 ggttgtgacg tcggccatga tgatgggccg gaagcgcacc ctgcaggctt ctatcatagc
      361 gccggccgct tcatgcccct tcttacggac cacctcggca tagtccacga tcactatggc
      421 gttgttgacc actagaccca cgatcatcac caggccgagg aggccgtaca tcgaaaggga
      481 cgttcccgtc aaaagaagcg tcgggatgac accgatcacc gacaggggga tcgtaagcat
      541 gatcgccagg gagaacaggt atgactccag tatgccggcg atcatcagga atgtcagcac
      601 gacggccatc gcgaaggcga tgaccatcct tcggaagttt tcctggatgt tctggatctc
      661 ccccccgata atgaacctgt atcctgtcgg cagctggatc tcgtccattc gcggcgtgat
      721 gtccctgaag acctccccga cggtgcgacc tgctacatcg gcctggacgc ttattttgcg
      781 ctgccggtcc agccggtaga tggccgtggg ggaaggctcg taggcgacgg cggccaactc
      841 gttgaccggg acgaacccat ggggtgtcca catgggaagc aggggaaccg cctcggggga
      901 gtcgacccac cgtgacgcca gtttgaccag gatgtcgtat tcgtagccct gttcccggta
      961 gaccccggcc ttaataccgc tcaggtaacc cctgagttca tcggacacat cggaaagggt
     1021 cacccccatg gaggtgagtt tgaggtcctc ggggatgagg ttaaggtcga acctccccac
     1081 cttccagtcc gtatccaggt cggccagccc cgggatgtcc cggatcttct ccatgagttc
     1141 cgccgctatg ccgttgagta catcgaggtc gggcccgctt atggttatct gtacgggttt
     1201 tctgcttccg ccgccttgac cggaagcgac attgatcgtc acgttcgtgt cgggaagacc
     1261 ggcaaatttt ttcctcaggt ccgaggcgat ggcgaaggcg ctcttccttt catcggcgtc
     1321 gaccaggtag acccttagga ccccgctgtt gacgctggaa agcctcctgc ctcccacggt
     1381 ggagctcatg gacttcacat agggttgctc acgcaaaaag gcctccactt tccttacctg
     1441 ctcatcggta gcctccagcg atgccgtgct cgatagttcc aggttgatcc tgatgacccc
     1501 ttcgtcgacc ctggggaaga gttcaaaccc caccctgggt atcaaaagga gcgatccggc
     1561 gaagagcagg ccgaagaagc cgatggtcac ccagggatgc ctcatggctt taccgacgta
     1621 cacctcctgg aaaacctcga gttcacggta gatccaatac caccaggccg tgaccttttt
     1681 ggcgaaggga gaaacgtgta ccgagccgcc tagcctggcg gcggccatcg gggtgaccgt
     1741 gaaggccccc cagagagaga agagtgtcga gaagaccacc gtcagggcaa attggttgaa
     1801 gaactggccc acgatcccgc ccataaaggc tatgggtatg aaaacgccca ggttggtggc
     1861 cgtggtcgag aggaccgcca gggatatctc cctggtcccc tcctccgccg cagtgagagg
     1921 gtccatgccc agctcacggt acctgtagat gttctccagg acgaggatgg cgttgttgac
     1981 caggacccct accgagatgg ccaaccccat ggtactcatg acattcaggg tgtacttgta
     2041 gaagtacatg gggatgaagg tggcaattat tgaggtgggc atggcgatcg ccacggcaag
     2101 ggtcgatccg aactgcctga ggaagaggta caggaagagg gccgtcagga gagtccctat
     2161 tatcatgtcc ctgatgacgt tcctgaccga cttctccacg aattcgctgg aatcgtagac
     2221 gacttccagt tccaggcttt tgcccagggt aggtgcgatc ttatcaagct ccgccctgac
     2281 ttcgccggag accttgacga tattggcgtt gggcctggcg atcagttcca gcagtatgga
     2341 atccctgcca tcgaacctgg cccggttcct ctcatcggcg agggcatcga cgacggtcac
     2401 cacgttcccc agctttatta ccctgccgtc gcccacgggg atgcccaggt cctcgaactt
     2461 gctcgactgg tccggctcac cgacgatgcg gaccgaggtc tccttccccc caagggtgat
     2521 gttccctgag ggagagttga agttcgaagc cttgaccagc gatgccagtc tcctgagact
     2581 caggttgtgc gcgatgaggc tggaggggtc ggcatcgagg tggatctccc tcaccctccc
     2641 gccgctgatg tcaacgcttg ccataccgat gatctgggtc agccttttgg cgacctggtc
     2701 ctccacgata tccctggcct ggtatgaagg gagggctgtc ttggcgacca ccatcatgaa
     2761 gggctgggca ttgatgtcca tcttgtccac cacgggttcg tcgg
//
LOCUS       DAOSRG010000153         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_32523_length_2804_cov_11.782466, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000153 DAOSRG010000000
VERSION     DAOSRG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32523_length_2804_cov_11.782466"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLM30_05415"
     CDS             <1..941
                     /locus_tag="PLM30_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54644.1"
                     /translation="EVVDIAEKTFKGFGEGTTINPTKVGLDLGETAEYPPYEGFMNAM
                     PAYVGWLDTAGIKWAGGFLGERKKLGLPYITSLILLIDPRVGNFKAVMDGEWITSMRT
                     GAQTAVSLKYLHKGPSIRLGLYGAGGQGHTQTEAVAAVFDIEEVRVFDINRESALKYA
                     EDMKDIVKGKVTVVDDPRDAAWGDAIVCVTQSKEKFLRNEWIARGTNVFPMGSYQEVD
                     DDFILSADRVIVDHIGQCLHRGALKGLVEAGKFQESDIDATIGEVVAGKKPGRLTGDD
                     RVLCIPIGTGAMDIAVATVILERAREKGLGGHFDFV"
     gene            complement(979..1587)
                     /locus_tag="PLM30_05420"
     CDS             complement(979..1587)
                     /locus_tag="PLM30_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR54645.1"
                     /translation="MAGVFEKVSSDNENNQIDLLIGRRIRAIRRGAQLTLDVLAQRTG
                     LSKALLSKIENGKVSSPISTHAKIARSLGVSLSELLREAEETRFLLIRKEERKPRSSR
                     KTPYGYSFESLGARWPNKSWSPFLMTYEPLPEPHTSPGFNYEGEEFIYVLEGRLEFFC
                     GESKYELDPGDCLFVDGSLPHGGRALGDKKCLALLVVLPRNP"
     gene            1685..>2804
                     /locus_tag="PLM30_05425"
     CDS             1685..>2804
                     /locus_tag="PLM30_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOR54646.1"
                     /translation="MKEFILRKVKVRKVAFGKYTEVKNGCLTIEAAALTEVLLEDRQM
                     KSVDIRIASPGDSVRIVPVKDIIEPRARLSEEESPFPGVFSDPHVAGEGVTVSLDGVC
                     VVTTGKMVNFQEGVIDMSGPGAEFSTFSRKNNVVLVIEPVEGLSKHEHERVVRMAGLR
                     AAAFLGRAGIPAECEKELRIPGADLPGLYSMAPSLPRVLYVCQVIAQGLLHDNYIYGL
                     NAQGCLPLLMTPGEVLDGAIVSGNCAAPCHKHTTFHHQNNPIVEDLLKEHGKTLNFLG
                     VLVTPVKTALVEKVRICTQAFKVARMLGAQGVIISEDGGGNPENDLMLLDRLLEGAGI
                     KTVLVTDEYAGSDGASPGLADSTPEADAVVTNGNGNQRV"
BASE COUNT          582 a          848 c          885 g          489 t
ORIGIN      
        1 aggaagtggt cgatatcgcc gagaagacct tcaagggctt cggcgagggc accacgatca
       61 accccaccaa ggtgggcctg gacctcgggg agaccgccga gtaccccccc tatgagggct
      121 tcatgaacgc catgccggcc tacgtgggct ggctggacac ggcggggatc aagtgggccg
      181 gcggtttcct gggcgaacgc aaaaagctgg gcctgcccta cataacctcc ctgatactcc
      241 tcatcgaccc cagggtgggc aacttcaagg ccgtcatgga cggcgagtgg atcacctcca
      301 tgcgcaccgg cgcccaaacg gcggtttccc tcaagtacct ccacaagggg ccgtccatcc
      361 ggctcggact ttacggggcc ggtggccagg ggcacaccca gaccgaggcc gtcgccgccg
      421 tcttcgatat cgaggaagtc cgggtgttcg atataaaccg cgagtccgcc ctgaagtacg
      481 ccgaagatat gaaggatatc gtcaagggca aggtgaccgt cgtcgacgat cccagggatg
      541 ccgcctgggg ggatgccatc gtctgcgtca cccagtccaa ggagaaattt ctcaggaacg
      601 aatggatagc ccgggggacg aacgtcttcc ccatgggctc ataccaggag gtggacgacg
      661 atttcatact gtccgccgac cgggttatcg ttgaccacat cgggcagtgc ctccacaggg
      721 gcgccctcaa ggggctggtc gaggccggga agttccagga gtcggacatc gacgccacca
      781 tcggcgaggt cgtggcaggg aaaaaaccag gacggctcac cggcgacgac cgggtcctct
      841 gcatccccat cgggacaggc gccatggaca tagccgtcgc cacggtcata ctcgaaaggg
      901 ccagggagaa aggattgggg ggtcatttcg acttcgtgtg aggcctaagg gcacacccct
      961 cccctgccgg gcccgccgct aggggttcct ggggaggacc accaggaggg cgaggcactt
     1021 tttatcgccc agggccctcc ccccgtgagg gaggctgccg tcgacgaaga ggcagtcccc
     1081 gggatccagt tcgtacttgc tctcgccgca gaagaactcg agcctgccct ccaggacgta
     1141 gatgaattcc tcgccttcgt agttgaaacc cggcgaggtg tggggttcgg gcaggggttc
     1201 gtaggtcatc agaaaggggc tccagctctt gttgggccag cgggcgccaa gggattcaaa
     1261 ggagtaaccg tagggcgtct tccgactgga gcggggcttc ctctcctcct tgcgaatgag
     1321 gaggaaacgg gtctcctcgg cctcccttag gagttcgctc agggagacgc ccagggaacg
     1381 ggcgatcttg gcatgggtgg atatggggga gctgactttg ccgttctcga tcttggaaag
     1441 gagggctttc gaaagcccgg tccgctgcgc gagcacatcc agagtcaatt gggcgccccg
     1501 ccggatcgcc ctgatccttc tcccaataag gaggtcgatc tggttgtttt cattgtcgga
     1561 agagaccttt tcaaaaaccc cggccatcgg gcgacctcct tcaaaaggcg gatttgttga
     1621 atcaatggaa acctattcta gcacctgaag ggctcgaaag acaccgatga aggaggatcc
     1681 tgccatgaaa gaattcattt taaggaaggt caaggtccgc aaggtggcct tcgggaaata
     1741 taccgaggtg aaaaacggtt gtcttaccat cgaagccgcc gccctcaccg aggtcctgct
     1801 ggaggaccgg cagatgaaaa gcgtcgatat ccggatcgct tcccccggtg atagcgtccg
     1861 catcgtcccc gtgaaggaca tcattgaacc ccgggcgagg ctttcggaag aggagagccc
     1921 cttcccgggc gttttttcgg acccccacgt ggccggtgag ggcgtcaccg tctccctcga
     1981 cggggtgtgc gtcgtgacaa cggggaaaat ggtcaacttc caggaagggg tcatcgacat
     2041 gtcggggccc ggcgcggagt tctccacctt ttcgcgcaag aacaacgtag tcctggtcat
     2101 cgaacccgtc gaggggctca gcaagcacga gcacgaacgg gtggtcagga tggccggcct
     2161 cagggccgcg gcctttttag gaagggcggg catcccggcc gagtgcgaaa aggaactccg
     2221 cattcccggg gcggacctcc ccggccttta cagtatggcc ccctcccttc ccagggtgtt
     2281 gtatgtatgt caggtcatag cccagggact cctccatgat aactacatct acgggctcaa
     2341 cgcccagggg tgcctacccc tgctcatgac ccccggcgag gtgctcgacg gggccatcgt
     2401 gagcggcaac tgcgccgccc cctgccacaa gcacacgacc ttccaccacc agaacaaccc
     2461 catcgttgag gacctcctca aggaacacgg taaaaccctc aacttcctgg gggtcctggt
     2521 cacacccgtc aagaccgccc tggtcgagaa ggtcaggatc tgcacccagg ctttcaaggt
     2581 ggccaggatg ctgggcgccc aaggagtgat aatttccgag gacggtggcg gcaacccgga
     2641 aaacgacctg atgctcctgg atcgcctgct cgaaggcgca gggataaaga cggtgctggt
     2701 caccgacgaa tacgccggct ccgacggggc ttcaccgggc ctggccgaca gcacgcccga
     2761 ggcggatgcc gtagtcacca acgggaacgg aaaccagagg gtga
//
LOCUS       DAOSRG010000154         2786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_32810_length_2786_cov_8.705969, whole genome shotgun sequence.
ACCESSION   DAOSRG010000154 DAOSRG010000000
VERSION     DAOSRG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32810_length_2786_cov_8.705969"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PLM30_05430"
     CDS             complement(<1..1202)
                     /locus_tag="PLM30_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR54647.1"
                     /translation="MKLHVLGAAGEVTGSNYILESGRTRLMVDCGLHQGGDEDKNREP
                     FAFDPSTIDVVLLTHAHLDHTGRVPLLVKMGFKGKVIATAPTVDLTEVLWRDAAKIQK
                     EDAEWKTRKNRRKGLDPEEPLYTEEDVEKASDLLLPVAYDDMLEPVPGFRVRFRDAGH
                     ILGSANIEVWVDDGDGEVKIVFSGDLGPQQTVMERTPSVIEDADYVVIESTYGDREHR
                     TNEESREEFREAMKVMIQDHGKVLIPTFVVDRAQRVLYEIMLMQKDGILPPGIPIYFD
                     SPMGEKATEVYNKHLSLLSSEIQEQIRAGNDPFSPENLRIVSDVEESKRINEVKEAVV
                     LAGSGMCNGGRIVHHLKNSLYLETTDVVFVGYQARSTLGRRLVEGEKYVRVAGEEVAV
                     RAKIHTING"
     gene            1324..1839
                     /locus_tag="PLM30_05435"
     CDS             1324..1839
                     /locus_tag="PLM30_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOR54648.1"
                     /translation="MDLMGALLIFGARLLDVPLGTFRILLLVRGKKLQAASTAFFESG
                     IYLIALGYVLQQGVRGWLQIVAYAGGYAVGNFLGATLEEKILSGFVLVEAVGPVTDEM
                     LETVDRLRDEGFGTTVVTGSGKTGERMILKIICRRHDIGHVAQVIGNRGFVFASDVRA
                     VWGGYFRQKRK"
     gene            complement(1820..>2786)
                     /locus_tag="PLM30_05440"
     CDS             complement(1820..>2786)
                     /locus_tag="PLM30_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOR54649.1"
                     /translation="YPAGETGWYVVAAVSWDKLLGPMLRFNRWPLLVGLVGLLGLLSV
                     LALWKWLVAPLRALGTEVSTLHLGRDIPEGDDPGAVHEIRRLRVVLQQLARGAVERAE
                     LMKRYVGDIVRVQEEERSRIARELHDGPLQDVTALIHQIRLAKMEERELGEAPKRLEN
                     AEEGARHAVNDMRALCDELSPPWLDLGLSQALDELAERLSRQLGVKITVEAGIEEELP
                     PEVTLAFFRVVQESVHNSVRHGGATAVNVRFQREGEEVLLEVTDDGAGFDPPEDFEEL
                     RVKGHRGLANMSERMSLIGGRLEVIRLPGTGTLVRCAWRFTSSSA"
BASE COUNT          494 a          889 c          795 g          608 t
ORIGIN      
        1 ccgttgatgg tgtgtatctt ggccctgacg gccacctcct ccccggcgac ccggacgtac
       61 ttctcaccct ctacgagccg cctgccgagg gtactcctgg cctggtaacc gacgaaaacc
      121 acgtcggtgg tctcaaggta aaggctgttc ttgaggtggt ggactatcct cccgccgttg
      181 cacatcccac tgccggcgag gaccaccgct tcctttacct catttatcct tttggattct
      241 tccacatcgg agacgatcct caggttttcg ggtgagaagg ggtcattgcc cgccctgatc
      301 tgttcctgga tctccgatga caaaagcgac aggtgcttgt tgtagacctc cgtcgccttt
      361 tctcccatgg gggagtcgaa gtagatcggg atgccggggg ggaggatccc gtctttctgc
      421 atgagcatga tctcgtagag taccctttgg gccctgtcca ctacaaaggt ggggatcagg
      481 accttgccgt ggtcctggat catgaccttc atggcttccc tgaactcctc gcggctttcc
      541 tcgttggtcc tgtgctccct gtcaccgtag gtcgactcga tgaccacgta atcggcgtcc
      601 tcgataacgg agggggtcct ctccatcacg gtctgctggg ggccaaggtc gcccgaaaaa
      661 acgatcttga cctcaccatc cccgtcatcg acccaaactt cgatgttggc gctgcccagg
      721 atgtgccccg catccctgaa ccggaccctg aaacccggga ccggctccag catgtcatcg
      781 taggccacgg ggaggagcag gtcagaagct ttctcaacgt cctcctcggt gtagaggggt
      841 tcttcggggt ccagcccttt cctccggttc ttgcgggtct tccactcggc gtcctccttt
      901 tggatcttgg cggcgtccct ccagagaact tccgtcaggt ccaccgtcgg cgccgtggca
      961 atgaccttcc ccttgaaacc catctttacc agcaggggca cccggccggt gtgatccagg
     1021 tgggcatggg tcaggaggac aacgtcgatg gtggaagggt cgaaggcgaa gggttcgcgg
     1081 tttttatcct cgtccccccc ctggtggagc ccgcagtcga ccatgagcct ggtccttcca
     1141 gactcaagga tgtagttcga accggttact tccccggcgg cacccagtac gtgtagtttt
     1201 atcggcatgt cccggtgttc tcccctttcg gatcttcttt caattcccct cattttatac
     1261 tagaatccca aggcgggccc gttccgaccg ttacgatcac catgcccctc aggaggttac
     1321 tccatggatc ttatgggcgc attgctcata ttcggcgcac gtttactgga tgttccgctg
     1381 ggcactttca gaatactgct tctcgtcagg gggaaaaaac tccaggcggc ttcgacggcc
     1441 ttcttcgagt ccggtatcta ccttatcgcc ctgggctacg tccttcagca gggcgtcagg
     1501 gggtggttgc agatcgtcgc ctacgcgggc gggtacgccg tgggcaactt cctgggcgct
     1561 acccttgagg aaaagatcct gagtggtttc gtcctcgtcg aggcggtggg tccagtcacg
     1621 gacgagatgc ttgaaacggt cgacaggttg agggacgagg gtttcggcac gaccgtcgtc
     1681 acgggttccg gcaaaaccgg cgaacggatg atcctcaaga taatttgccg gcgccatgac
     1741 atagggcacg tcgctcaggt gatcggcaat cggggctttg ttttcgcctc cgacgtcagg
     1801 gccgtatggg gaggttactt caggcagaag aggaagtgaa cctccaggcg cacctcacca
     1861 gggtccccgt gccaggcagc ctgatgacct ccagccttcc cccgatgagc gacatcctct
     1921 cggacatatt ggccaggccg cgatgtccct ttacccggag ttcctcgaaa tcctccggcg
     1981 ggtcgaaacc cgccccgtca tcggtcacct cgagaaggac ttcctcccct tccctctgga
     2041 acctgacgtt cacggcggtc gcacctccat ggcggacgga attgtggacc gactcctgga
     2101 ccaccctgaa aaaggcaagg gtgacctccg gggggagctc ctcctcgatg ccggcctcca
     2161 cggtgatctt aacccccaat tgcctcgaaa gacgctccgc cagctcgtcc agagcctggg
     2221 acaggccaag atcgagccag gggggtgaaa gttcatcgca gagtgccctc atgtcattaa
     2281 cggcgtgacg ggcgccctcc tcggcatttt ccagccgctt gggagcctcc ccgagctctc
     2341 tctcctccat tttagccagc ctgatctggt ggatcagggc ggtgacatcc tggagcggac
     2401 cgtcatggag ctccctggct atgcgcgatc gttcttcctc ctggaccctg actatgtcgc
     2461 cgacatatcg tttcatcagt tccgccctct cgacggcacc cctggccagc tgctgcagca
     2521 cgactctcaa cctcctgatc tcgtggacag ccccggggtc atcgccctcg gggatatctc
     2581 tcccaaggtg aagggttgac acttccgtgc cgagggccct caagggcgcc accagccatt
     2641 tccagagggc gaggaccgac aacaggccca ggagcccgac gagcccgacc agcaggggcc
     2701 aacggttgaa gcggagcatg ggcccgagga gcttatccca ggatacggcg gccacaacgt
     2761 accacccggt ttccccggca ggataa
//
LOCUS       DAOSRG010000155         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33009_length_2774_cov_6.811695, whole genome shotgun sequence.
ACCESSION   DAOSRG010000155 DAOSRG010000000
VERSION     DAOSRG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33009_length_2774_cov_6.811695"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLM30_05445"
     CDS             complement(<1..856)
                     /locus_tag="PLM30_05445"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOR54650.1"
                     /translation="MGELISARGIHKYFPGVHALNDVTFNVGAGEIHALVGENGAGKS
                     TLVKILSGIHTPDSGEILFEGRRVDWKSPRDALKAGIAVIHQELSLAPHLSVAENIFL
                     GREPRKGPFLDRAALRDEARRILARVHAAFDVDREVRTLIPGERQLVEIAKALSLNVK
                     VLAMDEPTSSLSDRETENLFGLMGELRRQGVGMIYISHRLEEVFRIADTITVLRDGAN
                     AGGGPMGDFDQEDVVRLMVGRSVDTLFPRERPALGDTVLEVEGLSRDGVLKDISFKVR
                     KGEILGFSG"
     gene            1001..1927
                     /locus_tag="PLM30_05450"
     CDS             1001..1927
                     /locus_tag="PLM30_05450"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOR54651.1"
                     /translation="MVVGSVNMDLIVEAERPARPGETLLGGRFSMAGGGKGANQAVAA
                     ARLGASVAMVGAVGGDRFGEFMLSALEEEGVDCRHVATRNGEATGVAMITVFPGGENS
                     IIVAPGANATVTGGDLLEGGSLFEGADALLVQLELPPETAVEALRLGKTSRALTVFDP
                     APYAALPDEAWGFVDIAVPNRLELAGFTGTGDLEEGAGRLLEKGAGAVVVTLGPEGAM
                     IRRGREVKRVGAFPVEAVDATGAGDAFSAALAVSLAEGKPLYEAALFASAAGALACTV
                     VGAQPGLPAREQVEGLLRGPSGGPPGKLQGEV"
     gene            1932..2321
                     /gene="rbsD"
                     /locus_tag="PLM30_05455"
     CDS             1932..2321
                     /gene="rbsD"
                     /locus_tag="PLM30_05455"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOR54652.1"
                     /translation="MKRGGLLHPDLNYYVSMLGHTDRFCVADAGLPIPEEVPRVDLAF
                     VPGKPSFQDVLEAVLEAVVVERAFCAVQASAELKGSLKQNLGPGCALEEVPHEELKSM
                     LRNVRFVVRTGECTPYANVILQSGVFF"
     gene            2318..>2774
                     /locus_tag="PLM30_05460"
     CDS             2318..>2774
                     /locus_tag="PLM30_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54653.1"
                     /translation="MKAGKDGVLTRKVLFSPYVVLGSVLAVLCAALALMEPRFLTVSN
                     LVNILRQSSILGIIALGMTCVILVRGIDLSVGSVVALSSLVGAALVKGGAPFYAAWGA
                     ALLAGGACGALNGFTVAWMRVPSFIVTLGMMGAARGAALLFTGGAPISGF"
BASE COUNT          432 a          845 c          996 g          501 t
ORIGIN      
        1 ggcccgaaaa gccgagtatc tcacccttcc tgaccttgaa gcttatgtcc ttcagcaccc
       61 cgtcgcggga gaggccttcc acttcaagta cggtatcgcc aagggcgggt ctttccctgg
      121 ggaacagggt gtcgacgctg cggccgacca tgaggcgcac gacgtcctcc tggtcgaagt
      181 cgcccatggg gccgccgccg gcgttcgcgc cgtcccggag cacggtgatg gtgtcggcga
      241 tgcggaatac ttcctcgagg cggtgggaga tgtagatcat ccccacgccc tgacggcgaa
      301 gttcccccat gagcccgaaa aggttctccg tctccctgtc ggaaaggctc gacgtgggct
      361 cgtccatggc caggaccttc acgttgaggg aaagagcctt ggcgatctcc accagttgcc
      421 gctccccggg aatgagggtc cgcacctcgc ggtctacgtc aaaggcggcg tgaacccggg
      481 cgagtatcct gcgcgcctca tcgcgaaggg ccgcccggtc caggaagggt cccttccgcg
      541 gctcccgccc caggaagatg ttttccgcca ccgaaaggtg tggggccagg ctcagttcct
      601 ggtgtatcac ggcgatgccc gccttgaggg cgtccctggg gctcttccag tcaacccgcc
      661 gaccttcgaa gagtatttca ccggaatcgg gggtgtggat gcccgagagg atcttcacca
      721 gcgtggactt cccggcgccg ttctcgccga ccagggcgtg gatctcgccg gccccgacgt
      781 tgaaggtcac gtcgttgagg gcgtgcacgc cgggaaagta tttatggatc cccctggcac
      841 tgatcagttc acccaaaaag aagccccctt ccctccccga gcggcggtga accatgccat
      901 tctgataaaa aggcttataa tagacacagt aaagtataac ctttcgtgaa gcctgaaggg
      961 gcgccccttc aaaaaggagg taggggacct ggtcagggta gtggtcgtgg ggtcggtgaa
     1021 catggacttg atcgtcgagg ctgaaaggcc ggcccgcccc ggggagacgc tcctgggggg
     1081 gcgcttcagc atggccggtg gcgggaaggg cgccaaccag gcggtggcgg ccgcccggct
     1141 gggggcttcg gtggccatgg tgggggccgt gggaggcgac cggttcggag agttcatgct
     1201 gtcggccctg gaggaagagg gcgtcgactg ccgccatgtc gccacgagga acggtgaagc
     1261 caccggtgtc gccatgatca ccgtcttccc ggggggtgag aactccataa tcgtggcccc
     1321 cggggcgaac gccaccgtca cgggcgggga tcttctggag ggcgggtccc ttttcgaggg
     1381 ggccgacgct ctcctcgtcc aactggaact ccccccggag accgccgtcg aggccctgag
     1441 gctcgggaag acttcgcggg cactgacggt cttcgacccc gccccctacg cggcgctgcc
     1501 cgatgaggcc tggggtttcg tggacatcgc cgtccccaac aggttggagc tggccgggtt
     1561 caccggcacg ggagacttgg aggagggcgc cggtaggctg ctcgaaaaag gggcgggggc
     1621 cgtggtggtc accctcggcc ccgagggagc gatgatccgt cgcgggcggg aggtgaaacg
     1681 ggttggagcc ttccccgtag aggccgttga cgccaccggc gcgggcgacg ctttctcggc
     1741 cgccttggcc gtttcgctgg ccgaggggaa acccctttac gaggcggccc ttttcgcctc
     1801 ggcggcggga gcgctggcct gcaccgtggt cggggcccaa ccgggcctgc ccgcgaggga
     1861 gcaggtggag gggctgctgc ggggcccttc gggtgggccg cccgggaagc tccaagggga
     1921 ggtgtgaggc cgtgaaaaga ggaggtttgc tgcacccgga cttgaactat tacgtgtcca
     1981 tgctgggcca tacggacagg ttttgcgtgg ccgacgcggg cttgccgata ccggaagagg
     2041 tcccgagggt cgacctcgcc tttgtccccg gcaaaccttc gttccaggat gtgctcgagg
     2101 ccgtcctgga ggccgtcgtg gtggagcggg ccttctgcgc cgtccaggcc tcggcggaac
     2161 tcaagggctc gctcaagcag aacctcgggc cgggctgtgc gctggaagag gtcccccacg
     2221 aggagttgaa gtcgatgctg cggaacgtcc ggttcgtggt gcgcacgggg gaatgtacgc
     2281 cctacgcgaa cgtgatactc cagtcggggg tcttcttttg aaggcaggga aggacggtgt
     2341 tctcacgagg aaggtccttt tcagccccta cgtggtcctg gggtcggtcc tggccgtcct
     2401 ctgcgcggcc ctcgccctga tggagccccg gttcctgacg gtgagcaacc ttgtgaacat
     2461 cctgcgccag tcgtcgatac tggggatcat cgctctgggc atgacctgcg tgatcctggt
     2521 cagggggata gacctttcgg tgggcagcgt ggtggccctg tcctccctgg tgggggcggc
     2581 cctggtgaag ggaggagcac ccttttacgc ggcctggggc gcggctctcc tggcgggagg
     2641 ggcctgcggg gccctgaacg ggttcaccgt ggcatggatg agggtgccct cctttatcgt
     2701 gaccctgggg atgatgggag cggccagggg ggcggcgctg cttttcacgg ggggagcccc
     2761 catcagcggc ttcc
//
LOCUS       DAOSRG010000156         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33060_length_2770_cov_9.358379, whole genome shotgun sequence.
ACCESSION   DAOSRG010000156 DAOSRG010000000
VERSION     DAOSRG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33060_length_2770_cov_9.358379"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLM30_05465"
     CDS             complement(<1..235)
                     /locus_tag="PLM30_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR54654.1"
                     /translation="MEGVNSTFTIDTLYETLIEQSPLGIAVQMPDRKVARANRAFCEM
                     FGYSLDEVVGMDLDDLVARDEDLFAGAGELSDLV"
     gene            complement(222..692)
                     /locus_tag="PLM30_05470"
     CDS             complement(222..692)
                     /locus_tag="PLM30_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOR54655.1"
                     /translation="MGVNGPGRLVRAALIGGVYVALTLLFAPISFGPAQVRVSEALAL
                     LPWMWPEAVPGLFVGCVISNFVGGFGIIDVVFGSLATLGAAILTSRMPNKVLAAIPPV
                     AFNALVVGGYLSSILEIPVVPTMIYVGLGEVVACFGLGIPLLTLLERRFRRGGR"
     gene            complement(701..2332)
                     /locus_tag="PLM30_05475"
     CDS             complement(701..2332)
                     /locus_tag="PLM30_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR54656.1"
                     /translation="MENTRQGILELLLDLARIPSVTGSPGEAEAGRFIHDRLGEMDYF
                     REHPDDLRFIPVPGDPFGRGAVMAVVEAEPAVKGTVVLTGHFDVVGTGEFGPLERLAF
                     SPVEYTAALRERGLEGEAGEDLASGDFLFGRGVMDMKCGLAAEMALLCEAAADPSSLG
                     VNLLFLAVPDEEIGSAGMRAAVPALAGIRDERGLEYLGAILAEPGAAGSPDIEGEAIF
                     LGSVGKVMPFFYCVGAGTHVGRYFDGLSASLMASHLSVLVEGDPRLADRSGDETGPPP
                     ACLFLRDLRDGYSVTTPDRAAVFFNYITLTRAPAEVMGEMMKVAEEAAFTAFEQVKRS
                     ASKLGAAFDTPRPRVVTFEEFAHETEAALGGGTKARIRGLVEAMDPALDDRHRSLAVV
                     TEMLRWDPPGVPMVIVGFLPPYYPHRFNEEGSEGERRARKAAGHVIRRARREYGVDLS
                     LKEYFAGICDLCYLGFQGSAMDMLCVASNTPGWGSVYRVALGELKALDIPVLNLGPSG
                     KDPHKITERLRLSYSLEVFPGLLREAVLSLARQGG"
     gene            2421..>2770
                     /locus_tag="PLM30_05480"
     CDS             2421..>2770
                     /locus_tag="PLM30_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54657.1"
                     /translation="MGVVEKNTTRGAPVEVMKLKRPAVNTKLVILTLTHFLTDLHGTF
                     LATFVPVIVGRLGISYAQAGLLNSLSGMIHIVVQPMAGYISDTFSRPYAIIVGPLLTA
                     LGASMLPLGPTYGVT"
BASE COUNT          530 a          943 c          842 g          455 t
ORIGIN      
        1 aaaccaggtc cgacagctcc ccggccccgg cgaacaggtc ctcatcgcgg gcgaccaggt
       61 catcgagatc catccccacg acttcgtcca gggagtagcc gaacatctcg cagaaggccc
      121 tgttggcacg ggccaccttc ctgtcgggca tctgaacggc gatcccgagt gggctctgct
      181 cgatcagggt ttcgtagaga gtatcgatgg tgaaagtact gttaacgccc tccacggcga
      241 aaccgccttt ccaggagagt gagaaggggt atccccaggc cgaaacatgc aacgacctcc
      301 cccagtccga catagatcat cgttggcacc accgggatct caaggataga agagagatag
      361 cccccgacta cgagggcatt gaaggcgacg ggtggaatgg cggcgaggac cttgttgggc
      421 atcctcgagg tgagtatcgc ggccccaagc gttgccaggc ttccgaatac cacgtcgatt
      481 ataccgaaac caccgacgaa attggagatg acgcaaccga cgaaaagacc gggaacggcc
      541 tcgggccaca tccacgggag cagcgccagg gcttccgaca ccctgacctg cgccggcccg
      601 aaggagatgg gggcgaaaag cagtgtcagg gccacgtaga ccccgcctat gagggcagcc
      661 ctgaccagcc ggccgggccc gttgacaccc atagaagaac tcagcctcct tgccgcgcca
      721 gagagaggac ggcctcccgc aagagcccgg ggaaaacctc cagggagtag gagaggcgga
      781 gtctctcggt gatcttgtgg gggtccttcc cggagggccc caggttcagc accgggatgt
      841 cgagggcctt cagttcgccg agggctaccc tgtagacgct cccccacccg ggagtgttcg
      901 acgccacgca gagcatgtcc atggcgctcc cctggaaacc caggtaacag aggtcgcaga
      961 tcccggcgaa gtactccttc agggagaggt ccacgccgta ctcgcggcgg gccctcctga
     1021 tgacgtgccc tgcggccttt cgggccctcc tttcgccttc gctcccttcc tcgttgaagc
     1081 ggtgagggta atagggcggt aggaagccca ctatcaccat cggtacgcca ggggggtccc
     1141 accggagcat ttccgttacc accgccagtg agcggtgacg gtcgtccagg gcggggtcca
     1201 tggcttcgac cagacccctg atcctggcct tcgtcccgcc ccccagggcc gcttcggtct
     1261 cgtgggcgaa ctcctcgaag gtcaccaccc gcgggcgggg ggtatcgaag gcggccccga
     1321 gcttcgaggc gctcctcttc acctgttcga aagcggtaaa ggcggcttct tcggcgacct
     1381 tcatcatctc gcccattacc tctgccggcg ccctggtgag ggtgatgtaa ttgaagaaga
     1441 ccgccgcccg gtcgggggtg gtgacggaat aaccgtcccg gaggtccctc aggaaaaggc
     1501 acgccggcgg ggggcccgtc tcatcgccgg acctgtcggc caaccgcggg tcaccctcga
     1561 cgaggaccga aaggtgggac gccatgaggg aagcgctcag gccgtcgaag taccgcccca
     1621 cgtgggtccc cgcgccgacg caatagaaga agggcatcac cttgccgacg gaacccagga
     1681 agatcgcctc cccctcaata tcgggagagc ccgccgctcc cggttcggcc aggatggccc
     1741 ccaggtattc gagacccctc tcatccctga tacccgccag ggcggggacg gcggcccgca
     1801 tgcctgcgga cccgatctcc tcatcgggga cggcgaggaa aagcaggttg acgccgaggc
     1861 tggaagggtc ggccgccgct tcacagagca gggccatctc cgccgccagg ccgcacttca
     1921 tgtccatcac gccgcggccg aagagaaagt caccggaggc caggtcctcg ccggcctcgc
     1981 cttcgaggcc ccgctccctc agggcggcgg tatactccac tggggagaag gccagtctct
     2041 ccagggggcc gaactccccc gtccccacca cgtcgaagtg ccccgtgagg accaccgtac
     2101 cctttacggc cggttcggcc tcgacgacgg ccatgacagc ccccctgccg aaggggtccc
     2161 ccgggacggg gatgaacctg agatcatcgg ggtgttcacg gaaataatcc atctccccga
     2221 gccggtcgtg gatgaaccta ccggcctcgg cctccccggg ggaaccggtg acgctgggta
     2281 tccgggccag atccaggagt agttcaagta tgccctgcct cgtattttcc atgaccggtc
     2341 ctctcttttt accggggtga agggatggcc caatcatacc accccccccg gataggcctg
     2401 tccatgtata cagtgataca atgggtgtcg tcgaaaaaaa caccacgagg ggagccccgg
     2461 tcgaggtcat gaaactgaaa cgccccgccg tcaacaccaa gctggtcatc ctgacgctga
     2521 cccattttct caccgacctc cacgggacct tcctggccac cttcgtcccg gtgatcgtcg
     2581 gtcggctcgg gatatcctac gcccaggcag gactgttgaa ttccctctcg gggatgatcc
     2641 acattgtggt ccagcctatg gccggttaca tctcagacac cttctcccgc ccctacgcga
     2701 taatcgtggg ccccctgctg accgccctcg gggcctccat gctccccctt ggccccacct
     2761 acggcgtcac
//
LOCUS       DAOSRG010000157         2768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33085_length_2768_cov_7.220789, whole genome shotgun sequence.
ACCESSION   DAOSRG010000157 DAOSRG010000000
VERSION     DAOSRG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33085_length_2768_cov_7.220789"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLM30_05485"
     CDS             <1..959
                     /locus_tag="PLM30_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOR54658.1"
                     /translation="NIDISEKRRPQDGRILIKTLNKNIDLRVSTLPTIYGEKVVLRLL
                     DQSNAMVGLEKLGLETDDRVIVDGIIAAPYGIVLVTGPTGSGKSTTLYSVLERLSKPE
                     VNIITVEDPVEYTMTGINQIQVNEKAGLTFGEALRSILRQDPDKVMVGEIRDLETAQL
                     AVRAALTGHLVLSTLHTNDAPSASIRLVEMGIAPFLVSSSLLAVIAQRLVRKLCVECR
                     QEYTIPDKTADDLGIPRGSTAYKPMGCEVCRGTGYKGRSGIYEIMKVDEEIQNLILDG
                     AAGHRIQQEAVREGMRTLRDSGLRKVLDGVTSLEEVLQVTLN"
     gene            981..2063
                     /locus_tag="PLM30_05490"
     CDS             981..2063
                     /locus_tag="PLM30_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOR54659.1"
                     /translation="MVISLQAVLAETIRKQATDIHLGTGLHPTVRIDGDLLALTSYGI
                     LNAEDVRSMTGEVLTRKQIEELEEKLEMDFSFTFTAPTGESSRFRGNCFFERGNPGMA
                     LRTIPTKIRTIAELKLPPRLEEVTKKHRGLFLVTGPTGSGKSTTLASLIQEINHTRAC
                     HIVTIEDPIEYLHTSVMALIHQREVGTDTWSFAEALKRVLRQDPDVILIGEMRDLETI
                     AAAVTAAETGHLVFATLHTQGAAQTVDRIIDVFPPYQQQQIRQQLSNVLIGACTQQLI
                     PTPGGGRVVATDLMFATPAVRNLIREAKVSQMVSVMQTGSSFGMHTMDQDLARLVRDG
                     VIPFDMARARAHDPKDLERLVFETAF"
     gene            2161..>2768
                     /locus_tag="PLM30_05495"
     CDS             2161..>2768
                     /locus_tag="PLM30_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR54660.1"
                     /translation="MRLKYKARNPKGDTVSGFMDADSQAQAAAIIRDRGLIPLSLEAA
                     TGARKLSFMDRLRMISTVSLKDRAVMFRQLATMISSGINLGSALEILGEQTKNQRLAA
                     SIREVKKQVDGGMSFSAAMRTQKVFSVLMVSIVRAGEEGGVLDSSLERLATFLEKQQE
                     LRSKIYSAVSYPAVVVTFALGVVYILITFIVPRFAQVFDSMG"
BASE COUNT          564 a          894 c          869 g          441 t
ORIGIN      
        1 ccaacatcga catctccgag aagaggcgtc cccaggacgg caggatcctg ataaaaacgc
       61 tgaacaagaa catcgacctc cgcgtgtcga cgctgcccac catatacggc gagaaggtcg
      121 tccttcggct cctggaccag tccaacgcca tggtaggcct ggaaaaactg ggcctcgaga
      181 ccgacgaccg cgtcatcgtc gacggcatta tcgcggcccc ctacggcata gttctcgtca
      241 cggggcccac ggggagcggg aagtcgacga ccctctactc cgtcctggag aggctgagca
      301 agcccgaggt gaacatcatc accgtagagg accccgtgga gtacaccatg actgggataa
      361 accagatcca ggtcaacgag aaagcggggc tcaccttcgg cgaggcgctg cgctccatcc
      421 tccggcagga ccccgacaag gtgatggtcg gggagatccg ggacctggag acggcccaac
      481 tggccgtcag ggcggccctc actggccacc tcgtgctctc cacgctccat accaacgacg
      541 cccccagcgc ctccataagg ctcgtggaga tggggatagc ccccttcctg gtctcgtcgt
      601 cgctgctggc ggtcatcgcc cagaggctcg tccgaaagct ttgcgtggag tgcaggcagg
      661 agtacaccat ccccgacaag acggccgacg acctggggat ccccaggggt tccaccgcct
      721 acaagcccat ggggtgcgag gtctgccggg ggaccggtta caaggggcgc tcgggcatct
      781 acgagataat gaaggtcgac gaggagatcc agaacctcat actggacggg gcggcgggcc
      841 acaggataca gcaggaggcc gtccgggaag gcatgaggac cctgagggat tcgggtctga
      901 ggaaggtgct cgacggcgtc accagcctgg aggaggtcct ccaggtgacc ctcaactgaa
      961 cgaagggcgg gggggcggcg atggttatat cccttcaggc ggttctggcg gagacgatcc
     1021 ggaagcaggc caccgatatc cacctcggca cggggctcca ccccacggtg aggatcgacg
     1081 gcgacctcct ggcgctgacg tcctacggca tcctgaacgc cgaggacgtc cgctccatga
     1141 cgggggaggt cctgacccgg aagcagatag aggaactgga ggagaagctg gagatggact
     1201 tcagcttcac cttcacggcc cctacggggg agagttcccg cttcaggggc aactgcttct
     1261 tcgagcgggg caaccccggg atggccctga ggacgatacc gacgaagata aggaccatcg
     1321 ccgaactaaa acttcccccc cgcctggagg aggtcacgaa aaagcaccgg gggctcttcc
     1381 tggtgacggg ccccaccggg agcggcaaga gcaccaccct ggcctccctc atccaggaga
     1441 taaaccacac cagggcgtgc cacatagtca ccatcgagga ccccattgag tacctccaca
     1501 cctcggtgat ggccctcatc caccagaggg aggtggggac cgacacctgg agcttcgccg
     1561 aggccctgaa gagagtcctg aggcaggacc ccgacgtgat cctcatcggc gagatgaggg
     1621 acctggagac catcgccgcg gcggtgacgg ccgccgagac ggggcacctc gtcttcgcca
     1681 ccctccatac ccagggcgcg gcccagactg tggaccgcat catcgacgtc ttccccccct
     1741 accagcagca gcagatacgc cagcagctca gcaacgtgct catcggggcg tgcacgcagc
     1801 agctgatccc caccccgggc ggggggaggg tggtggccac ggacttgatg ttcgccacgc
     1861 cggccgtgag gaacctcatc cgcgaggcca aggtctccca gatggtgagc gtcatgcaga
     1921 cgggctcctc cttcggcatg cacaccatgg accaggacct ggcccgcctc gtccgtgacg
     1981 gggtcatccc cttcgacatg gccagggcca gggcccatga ccccaaggac ctggagaggc
     2041 tggtcttcga gacggccttt tagcggaatg acgcgtcgta ttgacttgcc ttcatggtat
     2101 tcttataatt gacctgtaat aatatgatag tgctatctta accgaccggg ggtgcgctcg
     2161 gtgaggctca agtacaaggc ccgtaacccc aagggtgaca cggtgagcgg cttcatggac
     2221 gccgacagcc aggcccaggc cgccgcgatc atccgcgacc gcggcctgat ccccctttcc
     2281 ctcgaggccg ccacgggggc gcggaagctc tccttcatgg accgcctcag gatgataagc
     2341 accgtctccc ttaaggacag ggccgtcatg ttccggcaac tggccacgat gatatcctct
     2401 gggataaacc tggggtcggc ccttgagata ctgggggagc agacaaagaa ccagaggctc
     2461 gccgcctcca taagggaggt caagaagcag gtcgacgggg gcatgtcctt cagcgccgcc
     2521 atgcgcaccc agaaggtctt ctcggtgctg atggtctcca tcgtcagggc cggcgaggag
     2581 ggcggcgtcc tcgactcgag cctggagagg ctggccacct tcctggaaaa gcagcaggaa
     2641 ctgcggtcca agatatactc cgccgtcagt taccccgccg tggtggtcac cttcgccctg
     2701 ggggtggtct acatcctcat caccttcatc gtcccccgct tcgcccaggt cttcgactcc
     2761 atggggat
//
LOCUS       DAOSRG010000158         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33107_length_2766_cov_8.493914, whole genome shotgun sequence.
ACCESSION   DAOSRG010000158 DAOSRG010000000
VERSION     DAOSRG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33107_length_2766_cov_8.493914"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLM30_05500"
     CDS             <1..387
                     /locus_tag="PLM30_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOR54661.1"
                     /translation="SVPGAGAIIVGGLILFRAPGGELLNMSYNFMIGAVAVLAAFFLF
                     AAWAVWRSVKRRPVSGRDALVGSRVLAVSDLSPQGSVICHGEIWLARVPGDGRLASGE
                     TGVVESVEGLTLVVRPLAEGEEAPSR"
     gene            424..1215
                     /locus_tag="PLM30_05505"
     CDS             424..1215
                     /locus_tag="PLM30_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOR54662.1"
                     /translation="MIGHLLDLFMSMGSYVGALIIILVLLPSALRIVPEYQRAVVFRL
                     GRYVGTKGPGLVIVIPVIDRIYKVDLRVVTLDVPYQEVITRDNVPVKVNAVVYFRVID
                     PSRSVIEVENYIMATSQLSQTTLRSVVGRAELDEVLSARDKINIELQHIIDERTDPWG
                     IKVSAVEVKELELPEGMKRAMARQAEAERERRAKVIAAEGELQAAEKLTQAALKMEDS
                     PVTIQLRYLQTLREVAAENNSTTLFPIPIDMMRAFMETKKGGRGE"
     gene            1364..>2766
                     /gene="leuS"
                     /locus_tag="PLM30_05510"
     CDS             1364..>2766
                     /gene="leuS"
                     /locus_tag="PLM30_05510"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOR54663.1"
                     /translation="MGYDFLPIEKKWQERWAREGIFNVEKDPGKEKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMLARFLMMRGLKVLHPMGYDAFGLPAENAAIKYGIHPNKWTWDN
                     IEHMTEQLKRMGCSYDWRRRVETCNPDYYRWTQWLFLQFHHKGLASRKKAPVNWCESC
                     GTVLANEQVINDGRCWRCATPVVKKNLEQWFLHITDYAQELLDSLDDLPGWPEKVKIM
                     QRNWIGRSEGVRLSFALYGTDTRIETFTTRIDTIFGVTFLALSPEHPLVGYIAERSPA
                     RAEIESFVASCMSQTEREQAAAGAEKKGIDTGLMAVNPVNGQRVPIWIANYVLMEYGT
                     GAIMGVPAHDQRDFDFARKYSIDVIPVINPPEEAPLDGATMAEAFDGDGIQCNSGIFD
                     GMPSSEALPAMKEWFVSHGWGEKEVNFRIRDWLISRQRYWGAPIPIIYCDRCGAVPVP
                     ESDLPVELPLDIEMKPGS"
BASE COUNT          564 a          855 c          905 g          442 t
ORIGIN      
        1 agcgtgcccg gcgccggggc gatcatcgtg gggggcctca tcctcttcag ggccccggga
       61 ggtgaactgc ttaacatgtc gtacaacttc atgataggcg ccgtggcggt cctcgccgcg
      121 tttttccttt tcgccgcctg ggccgtctgg aggtccgtca agagacgccc cgtctccggc
      181 agggacgccc tcgtggggag cagggtcctc gccgtttccg atctctcgcc ccaggggagc
      241 gtgatatgcc acggggagat ctggttggcc agggtccccg gcgacggccg cctggcttcg
      301 ggggagacag gggtcgtcga gagcgtcgag gggctcaccc tcgtcgtaag gcccctggcc
      361 gagggcgagg aggccccgtc caggtaagag acgacaccga ttagaagggg aggttgagaa
      421 tagatgatcg gacacttgct cgacctgttc atgagcatgg gcagctacgt gggggcgctc
      481 atcataatcc tggtcctgct gccctcggcg ctgaggatcg tccccgagta ccagcgggcg
      541 gtggtcttca ggctcgggcg gtacgtgggc accaaggggc cgggcctcgt gatagtcata
      601 ccggtgatcg accgcatcta caaggtggac ctcagggtgg tcaccctcga tgtcccctac
      661 caggaggtca taaccaggga caacgtgccg gtgaaggtga acgctgtggt ctacttccgg
      721 gtcatagacc cgtccaggtc ggtcatcgag gtggagaact acatcatggc cacgagccag
      781 ctctcccaga cgacccttcg gtccgtcgtg ggcagggcgg agctcgacga ggtgctctcc
      841 gccagggaca agataaacat cgaactgcag catatcatcg acgagaggac cgatccctgg
      901 ggcatcaagg tcagcgccgt cgaggtcaag gaactggaac tgcccgaggg gatgaagagg
      961 gccatggccc gccaggccga ggccgagcgg gaacgccggg cgaaggtcat agctgcggag
     1021 ggcgaactcc aggccgccga gaaactgacc caggcggcgc tgaagatgga ggattccccc
     1081 gtgaccatcc agctcaggta cctccagacc ctcagggagg tcgcggcgga gaacaactcc
     1141 accaccctct tcccgatccc catcgacatg atgagggcct tcatggagac gaagaagggc
     1201 ggccgaggcg agtaaaagcc ctgcgcggcc gaagcagggg gaaggcgcgg gggggaaccc
     1261 cccgcgcctt ttgcgacatc tggcccggac ggatataata gggcctatcg cgaaaaccgc
     1321 agcactcaag cacgaaagag cgtcgctgca agggggacgg gacatgggtt acgactttct
     1381 accgatcgag aagaagtggc aggaacgatg ggccagggaa ggcatcttca acgttgagaa
     1441 ggacccgggc aaggagaagt tctactgcct ggagatgttc ccctacccca gcggcgccct
     1501 ccacatgggg cacctgagga actattccat cggggacatg ctggccaggt ttctcatgat
     1561 gagggggttg aaggtccttc accccatggg gtatgacgcc ttcgggctcc ccgccgagaa
     1621 cgccgccata aagtacggca tccaccccaa caagtggacc tgggacaaca tcgagcacat
     1681 gacggagcag ctgaagagga tggggtgcag ttacgactgg cggagaaggg tcgagacctg
     1741 caaccccgat tattaccggt ggacccagtg gctcttcctc cagtttcacc acaaggggct
     1801 ggccagcagg aagaaggccc ccgtcaactg gtgcgaaagc tgcgggaccg tccttgccaa
     1861 cgagcaggtc atcaacgatg gccgctgctg gcggtgcgcc acgccggtgg tgaagaagaa
     1921 cctcgagcaa tggttcctcc acatcaccga ttacgcccag gaactcctcg actccctcga
     1981 cgacctcccc ggttggcccg agaaggtcaa gatcatgcag cgcaactgga tagggcgctc
     2041 cgagggcgtc aggctcagct tcgcccttta cgggacggac acccgcatcg agaccttcac
     2101 cacccggatc gacaccatct tcggcgtcac cttcctcgcc ctttcgccgg aacaccccct
     2161 cgtgggatac atcgccgaaa ggtcacccgc cagggcggaa atagagagct tcgtcgcctc
     2221 ctgcatgtcc cagaccgagc gggagcaggc ggccgcgggg gccgagaaga agggcatcga
     2281 cacgggcctc atggcggtga accccgtgaa cggccagagg gtccccatct ggatagccaa
     2341 ctacgtcctc atggagtacg gcaccggcgc gataatgggg gtccccgccc acgaccagag
     2401 ggacttcgac ttcgccagga agtactccat cgacgtcatc cccgtgataa acccgcctga
     2461 ggaggccccc ctggacggcg ctaccatggc cgaagccttc gacggcgacg gcatacagtg
     2521 caactccggg atcttcgacg ggatgccctc atcggaggcg ctcccggcga tgaaggagtg
     2581 gttcgtctcc catgggtggg gagagaagga ggtcaacttc cgcatcaggg actggctgat
     2641 atcgaggcag cgctactggg gcgctcccat ccccatcatc tactgcgacc gctgcggcgc
     2701 cgtgccggtc cccgagagcg acctcccggt ggaactgccg ctggacatcg agatgaagcc
     2761 ggggtc
//
LOCUS       DAOSRG010000159         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33297_length_2754_cov_8.955539, whole genome shotgun sequence.
ACCESSION   DAOSRG010000159 DAOSRG010000000
VERSION     DAOSRG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33297_length_2754_cov_8.955539"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..2468
                     /locus_tag="PLM30_05515"
     CDS             57..2468
                     /locus_tag="PLM30_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOR54664.1"
                     /translation="MKDFEKMGSFYLGRQVDDTTGSATGELLLYDAKDLTTHGMIIGM
                     TGSGKTGLAISLIEEALIDEIPVIAIDPKGDLPNLLLTFPELAPGDFLPWVSPQEASR
                     EGLTPEELARKTAETWKKGLGEWGIDGARIHKMRESVDLAVYTPGSSAGKMVSVLRSF
                     DAPPEAVRSDGDLFRERVQTTASSLLALIGIEADPLTSREHILISRVLEEKWSRGESL
                     DVPSLIGMIQLPPFSSLGVLDLETFYPTGARMELALKLNHLAAAPSFAPWMEGEPLDA
                     GRFLYAPDGKPRASIFTISHLSERERMFFVSMLLNEVLGWVRTQPGTSSLRAILYMDE
                     IFGFFPPVKNPPSKTVLLSLLKQARAYGLGVLLATQNPVDLDYKGIANTGTWFIGRLQ
                     TERDKARILEGLKGASEKGFPLQEIDRMISGLGKRVFLLHNVHEDRPVLFQTRWAMSY
                     LAGPVTREQIKTLAGDEASPATPATARPVTAPAAGTMPQASIESRVMVSGVNSVYLPA
                     PAGVEAEYAPFLGAFASVHFDDSKRSISETRDYAFAVEFDAGPVTVDWRNAFELEEDP
                     SRLAQGVPTPVMAEAPTAALDPKAYTRWGREFLKWLPQEARLAVMTCPSLKETARSDE
                     SRADFAARVALARREMRDTEVEKIRRKYASKLTTLQNRLMRAEQAIERETEQARHKKL
                     DTAVSFGTALAGAFFGRKVNTYSASRMGTAISKAARLQKESGDVARARETAEAVKQEM
                     AELEVAMEGEINALIPVDSPEMAIQEEFITPKASGMNLSLLGLLWLPYSRNSQGGLTP
                     LWGKR"
     gene            2491..>2754
                     /locus_tag="PLM30_05520"
     CDS             2491..>2754
                     /locus_tag="PLM30_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54665.1"
                     /translation="MKRRNILFLVLTILLAGVLLAWGRFGPAPHGRVGVPEDPGVKTP
                     EVEVTAVPSPGSPASSGRSGGAAPSPGFRPAQGEPFSREDLEAA"
BASE COUNT          511 a          890 c          908 g          445 t
ORIGIN      
        1 gggggtggcc ggaatactgt aaccgtttat acttctacca gaggaggtgg gagccaatga
       61 aggattttga aaagatgggc tctttttacc tgggtagaca ggtggacgac accaccggtt
      121 cggcgacggg tgaactgctc ctttacgatg ccaaggacct caccacccac ggcatgatca
      181 tcgggatgac cggcagcggc aagacgggac tcgccatttc actcatcgag gaggccctga
      241 tcgacgagat accggtgata gccatcgacc ccaagggcga cctgcccaac ttgcttttga
      301 ccttccccga actggcgccg ggggatttcc tcccctgggt gagcccccag gaggcttcca
      361 gggaggggct cacgccggaa gagctcgccc gaaagacggc cgagacctgg aagaaggggc
      421 tcggggaatg gggcatcgac ggcgccagga tccacaagat gagggagtcc gtcgacctgg
      481 ccgtctatac cccggggagt tccgccggga agatggtgag cgtcctccgt tccttcgacg
      541 ccccgccgga ggccgtcagg tccgacgggg acctcttccg ggagagggtg cagaccaccg
      601 cctcgagcct cctggcgctg atcgggatcg aggccgaccc gctgacgagc cgggagcata
      661 tcctcatctc cagggtgctg gaggaaaagt ggtcccgggg ggagtccctg gacgtaccct
      721 ccctcatcgg gatgattcag ctgccgccct tcagttccct cggcgtactg gacctggaga
      781 ccttctaccc caccggcgcg aggatggagc tggccctgaa gctgaaccac ctggccgccg
      841 cgccttcctt cgccccctgg atggaggggg agcccctcga cgctggacgg ttcctctacg
      901 cccccgacgg aaagcccagg gcatcgatat tcaccatcag ccatctttcc gagcgggaga
      961 ggatgttctt cgtctccatg cttctgaatg aagtgctcgg ctgggtcagg acccagcccg
     1021 gcacgtcgag cctgagggcc atcctctaca tggacgagat cttcggcttt ttcccgccgg
     1081 tgaagaaccc gccctccaag acggtcctcc tctccctgct caagcaggcc agggcctacg
     1141 ggctgggcgt cctcctggcg acacagaacc ccgtggacct cgactacaag ggcatagcca
     1201 acacggggac ctggttcatc gggaggctcc agacggagcg cgacaaggcg cggatcctcg
     1261 agggtctcaa gggggcgtcc gaaaagggct tccccctcca ggagatcgac aggatgatat
     1321 cggggctcgg caagcgggtc ttccttctgc ataacgtcca cgaggacagg cccgtcctct
     1381 ttcagacccg gtgggccatg tcctacctgg cggggcccgt gacgagggag cagataaaaa
     1441 ccctcgcagg tgatgaggcc tccccggcga ctcctgcaac ggcccggccg gtgacggcgc
     1501 ccgccgccgg taccatgccc caggcttcga tcgagtcgag ggtgatggtc tcgggggtca
     1561 actccgtcta cctgcccgct ccggcagggg tcgaggcgga gtacgccccc ttcctgggcg
     1621 ccttcgccag cgtccatttt gacgacagca agcgatcaat ctccgagacg agggactacg
     1681 ccttcgccgt ggagttcgac gccggccccg tgacggtgga ctggcggaac gccttcgaac
     1741 tggaggagga cccctcccgg ctggcccaag gggtccctac gccggtcatg gccgaggccc
     1801 caacggctgc gctggacccg aaggcctaca cccgttgggg gcgggaattc ctgaagtggc
     1861 tgccccagga ggcccggctg gccgtcatga cctgcccctc gctgaaggag accgccaggt
     1921 ccgatgaatc gagggccgac ttcgcggccc gagtggccct ggccaggcgg gagatgcgcg
     1981 acaccgaagt cgagaagatc cgccggaagt acgcctccaa actgaccacg ctccagaacc
     2041 ggctcatgag ggccgagcag gccatcgaga gggagaccga gcaggccagg cacaagaaac
     2101 tggatacggc cgtttccttc ggaacggccc tggcgggtgc cttcttcggc aggaaagtca
     2161 acacttattc ggcttcaagg atggggaccg ccatctccaa ggcggcgcgg ctccagaagg
     2221 agagcggcga cgtagcccgt gccagggaga ccgccgaggc cgtcaagcag gaaatggccg
     2281 aactggaggt cgcgatggag ggggagatta acgccctcat tcccgtggat tcgccagaga
     2341 tggcgatcca ggaggagttc ataaccccca aggcctcggg aatgaacctc tccctgctgg
     2401 gtcttttgtg gctcccctac tcacggaact cccagggagg gctcaccccc ctttggggaa
     2461 agcggtgata gcacgaccca aggaggtgcg atgaaaagaa ggaacatcct tttccttgtc
     2521 ctgaccatcc tcctggcggg ggtgctgctg gcctggggga ggttcggacc cgctccccac
     2581 ggacgggtgg gtgttcccga ggaccccggg gtgaaaacac cggaggtcga ggtgacggcc
     2641 gtgccgtcgc cgggaagccc cgcctcttcc ggccggagcg gcggggccgc ccccagtccg
     2701 ggtttccgtc ccgcccaggg ggagcccttc agccgggaag acctggaggc cgcc
//
LOCUS       DAOSRG010000160         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_33801_length_2725_cov_8.410112, whole genome shotgun sequence.
ACCESSION   DAOSRG010000160 DAOSRG010000000
VERSION     DAOSRG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33801_length_2725_cov_8.410112"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1221
                     /locus_tag="PLM30_05525"
     CDS             58..1221
                     /locus_tag="PLM30_05525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR54666.1"
                     /translation="MRETFLPFAKPDISEEAIADVADSLRSGWITTGPKALKFEKSFG
                     EYLGVPYALAVNSATAGLHLSLLAFGIGPGDEVLTTPMTFAATANTVILTGARPVLAD
                     IDPVTFNIRPELVKKALTPRTKALIPVHFAGRPCEMEALEEIAEKNGLVIIEDAAHAL
                     GASRRGRPIGSPARQRQAAVFSFHPTKNITTGEGGMVCTACEDAAERVSVLRQHGMSK
                     GAWNRYAATGTPHYDLLFPGLKYNMLDIQAAIGNDQLTRLASFNARRREIVSMYRKGL
                     EGLPGLALPPEPAQGDVHSWHILTPLIDIDYLGFSRDEFMARMRELNIGTALHYQALH
                     LFSYYGREYGWKRGDFPGAEFVSDRIVSIPLFPAMTDGDVADVIEAIRRVCGR"
     gene            1224..2177
                     /locus_tag="PLM30_05530"
     CDS             1224..2177
                     /locus_tag="PLM30_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR54667.1"
                     /translation="MSLEKASLDVSVVIPVFNEEESLPGLFERLFPVMEGLGKSWEVV
                     FVNDGSRDRSLALLLGEVDRHPGSVRVVDFNGNFGQHMAIMAGFSVSRGGAVVTMDAD
                     LQNPPEEIPRLLERMDAGHDVVGTIRSTRKDPLFRKFASKVVNRITNRITGLNLHDYG
                     CMLRAYSRRVVDLINSCPESTTFIPALAQKFALNPTEIEVGHSERELGTSKYSLFRLI
                     RLNFDLMTGFSLVPLQAVTMLGLLIAGLSFVFSIYMVLRRLFVGPEAEGLFTLMTINF
                     FLMGVTMFCVGIAGEYIGRIFQEIRKRPRFLVNRIYGDEEE"
     gene            2164..>2725
                     /locus_tag="PLM30_05535"
     CDS             2164..>2725
                     /locus_tag="PLM30_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOR54668.1"
                     /translation="MRKSERPRTVVFAYSEVGHRCLRALMGLRADIRGVFTYEDDRNE
                     NTWFSSVKDLAMAGGIPVFTPRGLGDAGAAALVEGLKPEVLFSFYYRDLIPADILAMP
                     RLGAFNMHGSLLPRYRGRACVNWAIIHGETETGATLHWMTPRPDEGDIVDQERVPIGE
                     RDTALEVMLKVADAAEKVLTRSLPLIE"
BASE COUNT          502 a          869 c          900 g          454 t
ORIGIN      
        1 cctggggcgg gtcggggata aaatgctggt cacaaacaag ggagatgaac cgtgacgatg
       61 agagagacct tcctgccctt cgcgaaacct gacatcagcg aggaagccat agccgacgtg
      121 gcggattcgc tgaggtcggg gtggataact accgggccca aggcgctgaa gttcgagaag
      181 tccttcggtg agtacctcgg cgtgccctac gcgctggcgg tgaactcggc cacggcgggg
      241 cttcacctgt cgctgctcgc cttcggcatc gggcccggcg acgaggtctt aacgacgccc
      301 atgaccttcg cggcgacggc caacacggtg atactgaccg gcgccaggcc cgtgttggcc
      361 gatatcgacc cggtgacgtt caacatcagg cccgaactgg tgaagaaggc cctcaccccg
      421 aggacgaagg ccctcatccc ggtgcacttc gcggggcgcc cctgcgagat ggaggccctc
      481 gaggagatag ccgagaagaa cggccttgtc atcatcgagg atgccgccca cgccctgggg
      541 gcgtcccgcc gggggcggcc catcggctcc ccggcgcggc agaggcaggc cgccgtcttc
      601 agcttccacc ccacgaagaa catcaccacc ggggaggggg gcatggtctg caccgcctgc
      661 gaggacgccg ccgagagagt ctccgtcctc aggcagcacg ggatgagcaa gggggcctgg
      721 aaccgctacg ccgccacggg gaccccccat tacgacctgc tcttcccggg cctgaagtac
      781 aacatgctcg acatccaggc cgccatcggg aacgaccagc tgacccggct ggcttccttc
      841 aacgcccgga ggcgggagat agtgagtatg tacaggaagg ggctggaggg cctgccgggc
      901 ctcgccctgc cacccgaacc cgcccagggg gacgtccact cctggcacat cctcaccccc
      961 ctcatcgata ttgactacct gggcttctcc cgggacgagt tcatggccag gatgagggag
     1021 ttgaacatcg ggacggccct tcactaccag gcgctgcacc tcttctccta ctacgggagg
     1081 gagtacggct ggaagagggg cgacttcccc ggcgcggagt tcgtcagcga caggatagtg
     1141 tcgataccgc tcttcccggc catgaccgac ggcgacgtgg ccgacgtgat cgaggcgatc
     1201 cggcgggtct gcgggaggtg agagtgtcct tggagaaggc atccctggac gtttcggtgg
     1261 tgatacccgt cttcaacgag gaggagtccc tccccggcct tttcgaaagg cttttccccg
     1321 tcatggaggg gctgggcaag agctgggagg tggtcttcgt caacgatggg agcagggacc
     1381 gctccctggc cctgctgctc ggagaggtcg accggcaccc cggctccgtc agggtggtgg
     1441 acttcaacgg caacttcggc cagcacatgg ccatcatggc ggggttttcc gtatcgaggg
     1501 gcggcgccgt ggtgaccatg gacgcagacc tgcagaaccc ccccgaggag atacccaggc
     1561 tcctggagag gatggacgcc ggccacgacg tggtggggac catccggtcc accaggaagg
     1621 accccctctt caggaagttc gcctcgaagg tcgtcaacag gatcaccaac aggataacgg
     1681 ggctgaacct ccacgactac ggctgtatgc tgcgggccta cagccggcgg gtggtggacc
     1741 tcatcaacag ctgccccgag tcgaccacct tcatcccggc cctggcccag aagttcgccc
     1801 tcaacccgac ggagatagag gtgggacact ccgagaggga gctggggacc tctaagtaca
     1861 gtctcttcag gctcatcagg ctcaacttcg acctgatgac gggtttctcc ctggtgccgc
     1921 tccaggccgt cacgatgctg ggactcctga tcgcggggct gagcttcgtc ttctccatct
     1981 acatggtcct gaggaggctc ttcgtggggc ccgaggcgga aggccttttc accctgatga
     2041 cgataaactt cttcctgatg ggggtcacca tgttctgcgt cggcatcgcc ggggagtaca
     2101 tcggccggat cttccaggag atacgcaaac ggccccggtt cctggtgaac aggatctacg
     2161 gtgatgagga agagtgaaag gccgaggacc gtcgtcttcg cttacagcga ggtggggcac
     2221 cggtgcctca gagcgctgat gggcctgagg gccgatatta ggggcgtctt cacctacgag
     2281 gacgacagga acgagaacac ctggttcagc tccgtgaagg acctggccat ggcgggcggc
     2341 atacccgtct tcaccccccg cggcctcggc gacgctggcg cagcggccct cgtggagggc
     2401 ctgaagccgg aggtgctttt ctccttctat taccgcgacc tcatcccggc ggacatcctc
     2461 gccatgccgc gcctgggggc cttcaacatg cacggctccc tgctgcccag gtaccggggg
     2521 agagcctgtg tgaactgggc cataatccac ggtgagaccg agacgggggc caccctccac
     2581 tggatgacgc cccgccccga cgagggggat atcgtcgacc aggagagggt ccccatcggg
     2641 gagagggaca ccgccctgga ggtgatgctc aaggtggccg atgccgccga gaaggtgctc
     2701 acccggagcc tccccctcat cgagg
//
LOCUS       DAOSRG010000161         2710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34057_length_2710_cov_8.706968, whole genome shotgun sequence.
ACCESSION   DAOSRG010000161 DAOSRG010000000
VERSION     DAOSRG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34057_length_2710_cov_8.706968"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLM30_05540"
     CDS             complement(<1..740)
                     /locus_tag="PLM30_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOR54669.1"
                     /translation="MAGKKKIPLVWKIAIGFVAGILAGVAFGEKISVIAPVGTIFIAL
                     LQMLVVPLVFSSLVVGTASIGDPLSLGRVGVKTLVLYLVTTAIAIAIGLTLGNIINPG
                     AGMGLGADLSAFQIPTPPKIGQVIIDLFPKNPVASMAQANMLQIIVFALFLGMAAIFA
                     GEKGKPLINVMDSLAETMYKLTGMVMQVAPYGVFALIATTVAKYGISVMAPFGKVIFA
                     VYLGCFLHAAIVYSGLLTIWAHFSPLRF"
     gene            1049..2488
                     /gene="proS"
                     /locus_tag="PLM30_05545"
     CDS             1049..2488
                     /gene="proS"
                     /locus_tag="PLM30_05545"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR54670.1"
                     /translation="MARNIVPRKEDYSQWYLDVIKTAELADYAPVRGCMVIRPNGYAL
                     WESIQGHFDEVFKETGHVNAYFPLLIPQSFLEKEAEHVEGFAPECAVVTHAGGEELEE
                     PLIIRPTSETVIGHMYSKWVQSWRDLPILINQWCNVMRWEKRSRLFLRTSEFLWQEGH
                     TAHSTSEEAMEETLKMLGVYRDIMENVLGLPILAGEKSEGERFPGADNTYSCEAMMSD
                     GKALQAGTSHFLGQNFARAFDITFQNREGELDYAWTTSWGSSTRLIGAIVMTHSDDDG
                     LVLPPRVAPVKAVIVPISPDQSFLDEKLLPRAREAAERLDTLLGKRSVKIDNDFHLRP
                     GDRFFANLQKGIPLRLELGEKEFGKGVFQAVRRDTGERLDIPWDQVEERVPALLEEIQ
                     QDLFAKALAFREKNTVRVGDFGEFKEVFRGRGGFVEAFFAGGRDEEKAIKEETGATAR
                     CFPMDKEERGDCFFTGRPGARLAVFARAY"
BASE COUNT          571 a          793 c          857 g          489 t
ORIGIN      
        1 aagaagcgca ggggcgaaaa atgtgcccag atggtgagaa gccccgagta gacgatggcc
       61 gcgtggagga agcagcccag gtaaacggcg aagatgacct ttccgaaggg tgccatcacc
      121 gatatcccgt acttcgccac cgtcgtggcg atcagggcga acacgccgta gggtgccacc
      181 tgcatgacca tgcccgtcag cttgtacatg gtttccgcta aggagtccat gacgttgatg
      241 aggggtttgc ccttctcacc ggcgaagatc gcggccatcc caaggaagag ggcgaagacg
      301 attatctgga gcatgttggc ctgcgccatg gaggccacgg ggttcttcgg gaaaagatct
      361 atgatgacct ggccgatctt gggtggggtc ggtatctgga aggccgagag gtcggcccca
      421 aggcccatcc ccgcgcccgg gttgatgata ttgcccaggg tgagcccgat ggctatggcg
      481 atggccgtag tgaccaggta gaggaccaga gtcttgacgc ccaccctgcc cagggatagg
      541 gggtcaccga tcgaagccgt gcccaccacc aggctcgaga agaccagggg gacgacgagc
      601 atctgcaaaa gcgctatgaa gatggtgccg acaggggcga tgactgagat cttttcgccg
      661 aaagccacac cggcaaggat gccggccacg aaaccgatcg cgatcttcca gacaaggggg
      721 atcttcttct ttccagccat ttcaatccca ctcctttcaa ttgctcacgg gttttcggct
      781 ttctctctcg tttttctttc ctggttccag cgccttttca tcacctccgg cgggggacag
      841 gaagacaggg ggaaggggtg gaattgcgct cctttacaag ggtgattata tatcctcctg
      901 gtgcttttgt cctttcccgc ggctccccga ccgcccatag gggggtgaag gagggcttcg
      961 ccttgccccg tctccctttt gtgcctaaaa tgtgcggtat acctggaagg gcgtcggcaa
     1021 ggcgcgacgg gatgaggagg aaggacgaat ggcacgcaac atcgttccca ggaaagagga
     1081 ctattcccag tggtaccttg acgtgatcaa gacggcggaa ctggccgatt acgcgcccgt
     1141 gaggggctgc atggtcatca ggcccaacgg ttatgccctg tgggagtcga tccaggggca
     1201 cttcgacgag gtcttcaagg agaccggcca tgtcaacgcc tattttccgc tcctcatccc
     1261 tcagtccttc ctggaaaagg aagccgagca cgtcgagggc ttcgcgccgg aatgcgccgt
     1321 agtgacccac gccggcgggg aggaactgga agaaccgctg ataatccgcc ccacctccga
     1381 aacggtcatc ggccacatgt acagcaagtg ggtccagtcc tggagggacc tgcccatcct
     1441 catcaaccag tggtgcaacg tgatgagatg ggagaaaagg tctaggctct tcctcaggac
     1501 ctccgaattc ctctggcagg aagggcatac cgcccactcc accagcgagg aggccatgga
     1561 ggagaccctg aagatgctgg gtgtctaccg ggacatcatg gagaacgtcc tgggcctccc
     1621 catcctggcc ggcgaaaagt ctgaaggcga acgcttccct ggcgctgaca acacctattc
     1681 ctgcgaagcc atgatgagcg acgggaaagc tctccaggcc ggcacgagcc acttcctggg
     1741 gcagaacttc gccagggctt tcgacatcac cttccagaac agggagggcg aactggacta
     1801 cgcctggacc accagctggg ggtcctccac aaggctcatc ggggccatag tgatgaccca
     1861 ttccgacgac gatgggctgg tgctgccccc cagggtcgcg cccgtcaaag cggtgatcgt
     1921 ccccataagc cccgaccagt cctttctgga cgaaaaactc ctccccaggg cccgggaggc
     1981 ggcggaaagg ctcgacacac tcctcggcaa acgctccgtc aaaatcgaca acgacttcca
     2041 cctgaggccc ggcgaccgct tcttcgctaa cctccagaag ggcatacccc tccgcctgga
     2101 actgggcgag aaggaattcg ggaagggcgt cttccaggcg gtacggaggg ataccggcga
     2161 gcgcctggac attccctggg accaggtgga ggaaagggtg cccgcgctgc tcgaggagat
     2221 ccagcaggac cttttcgcca aggccctcgc tttccgggag aagaacaccg tcagggtcgg
     2281 cgacttcggg gagttcaaag aagtattccg cggccgcggt ggtttcgtag aggccttctt
     2341 cgccggcggc cgggatgagg agaaggccat caaggaggag acgggagcca cggcgcgctg
     2401 cttccccatg gacaaggagg aacggggcga ttgtttcttc accggccgtc caggggcgag
     2461 gctggcggtg ttcgccaggg cctattgagc cgccgttgct ttataattgc tttacggcag
     2521 agttccatga ccgacccgct gacccatcgg gatgaaacgg gctgggagga gagaacatgg
     2581 tgtccgagca aaccgtcggg gggacagtga tccggctccg caagggtgat atcacctgct
     2641 acgaaggaga agccatagtc aacgccgcca actcggccct ctggatgggc tccggcgtcg
     2701 cgggggccat
//
LOCUS       DAOSRG010000162         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34155_length_2704_cov_7.708192, whole genome shotgun sequence.
ACCESSION   DAOSRG010000162 DAOSRG010000000
VERSION     DAOSRG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34155_length_2704_cov_7.708192"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="PLM30_05550"
     CDS             <1..2278
                     /locus_tag="PLM30_05550"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOR54671.1"
                     /translation="YNDSYLERLFAFANLINTVEGGTHVSGFRTALTRAINESARRNK
                     VIRDKDPNLTGDDLKEGLTAVISVKLPEPQFEGQTKTKLGNGEIKGIVDSLFYDGLMA
                     EIDERPDILKPVVEKAVKARLAREAAKKARELVRRKTALAGLDLPGKLADCSSRNPEE
                     CEIFIVEGDSAGGSAKQGRDRNFQAILPLRGKILNVEKARIDKALDNQEIRTIIQALG
                     AGVGDDFNVDAVRYNKVFVMSVDGEEPTLVKDPHGMIRQVRIGEFVDRVIDGSLSPDG
                     YQVLCFDLTTQATRFRPLAKVIRHPVKEPLHEIRTAYGRTVKVTSSHSVFVCDGGEVA
                     LRRGCEVAPGDLLVAPRAIDLGEGAAPDFDLPGMAVPAPGDPAREDHPRRLQVDQDLM
                     TVLGLFTAAGILDEEGIGFALGTGKGSLEGELRNRFRKAFGVDLQQRRTGELAVSDPL
                     LPAVFRLLFDFDGEAPKGVPGIVFDAPADLKKAFLRGYFLVEGGVSGKGVDFRAPARD
                     LAAGILFLLSSMGIMASLHSRDRRVTLSAVEDLGLIEKVWRDHPLAGALLERVRGAAG
                     NENKRRFTPLRGDLVGLKVTGVSEVEPSSRWVYDFSVEEDENFICGMGGICCHNTDAD
                     VDGAHIRTLLLTLFYRFMPQLIERGHLFIAQPPLYRVQAGKEVHYCYSDKDLKALQDR
                     LKGRKLAILRYKGLGEMNPEQLWETTMDPATRISKRVEVEDAVSAEEYFSILMGDKVE
                     PRREFIEAHAKEVRNLDV"
     gene            complement(2458..>2704)
                     /locus_tag="PLM30_05555"
     CDS             complement(2458..>2704)
                     /locus_tag="PLM30_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54672.1"
                     /translation="EEEVIYENKVGAVLENLTTLPYDIVTGKMEKYPWDPEKTRFLSR
                     GGTAIGPWAFLDQNRGRTVMLYLDADGMVVMAEPVAM"
BASE COUNT          560 a          862 c          841 g          441 t
ORIGIN      
        1 gtacaacgac agttacctgg agaggctctt cgccttcgcc aacctcataa acaccgtcga
       61 gggcggcacc cacgtttcgg gcttccggac ggccctgact cgggccatca atgaatcggc
      121 ccggaggaac aaggtcatac gggataagga ccccaacctg accggcgacg acctgaagga
      181 gggccttacg gcagtcatat cggtgaaact ccccgagccc cagttcgagg gtcagaccaa
      241 gaccaagctg ggcaacggcg agatcaaggg catcgtggac tccctcttct acgacggcct
      301 catggccgag atcgacgaaa ggcccgacat cctgaagccc gtcgtcgaga aggccgtcaa
      361 ggccaggctc gccagggagg ccgccaagaa agccagggaa ctggtgcgcc gcaagacggc
      421 cctggcgggg cttgacctgc cgggtaaact ggctgactgc tcgagccgca accccgagga
      481 gtgcgagata ttcatcgtcg agggggattc ggcaggcggc tccgccaagc agggacggga
      541 ccgcaacttc caggccatcc tccccctgcg ggggaagatc ctcaacgtgg aaaaagcccg
      601 catcgacaag gccctggata accaggagat acgcaccata atccaggccc tcggggccgg
      661 cgtgggcgac gacttcaacg tcgatgccgt caggtacaac aaggttttcg tcatgagcgt
      721 cgacggggaa gaacccaccc tcgtgaagga cccccatggg atgatccggc aggtccggat
      781 cggtgaattc gtcgaccggg tcatcgacgg atccctgtcc cccgatgggt accaggtcct
      841 gtgctttgac ctaaccaccc aggccacacg gttcaggccg ctggccaagg tcatccggca
      901 ccccgtcaag gagccccttc acgagatccg taccgcctac ggcaggaccg tgaaggtcac
      961 atcgtcccac agcgttttcg tctgcgacgg gggagaggtc gccctgagga ggggctgtga
     1021 ggtagccccc ggcgacctgc tcgtagcgcc ccgcgccatc gacctgggtg aaggggccgc
     1081 accggacttc gacctcccgg gaatggccgt tccggcgccg ggagaccccg ccagggaaga
     1141 ccacccgcgg cgcctccagg tcgaccagga cctcatgacc gtcctgggcc tcttcaccgc
     1201 cgcgggcatc ctggacgaag agggcatcgg cttcgctctc ggaaccggca agggatccct
     1261 tgaaggagag ctccgtaacc ggttccgcaa ggccttcggc gtggacctcc agcagcgtcg
     1321 caccggtgaa ctggcggtct cggacccgct ccttcccgcc gtcttcagac tccttttcga
     1381 cttcgacggg gaggccccga aaggagtgcc cggcatcgtc ttcgacgccc cggcggacct
     1441 aaagaaagcc tttctccggg gttatttcct cgtagagggc ggcgtgagcg ggaagggcgt
     1501 ggacttcagg gcgcctgcca gggacctggc ggcgggcatc ctcttccttc tctcctccat
     1561 ggggatcatg gcatcgcttc actcgagaga ccgaagggta acgctgtccg ccgtcgaaga
     1621 cctcggcctc atcgagaagg tgtggcggga ccaccctctc gccggggcgc tgctcgaaag
     1681 agtccgcggg gccgccggca atgagaacaa gcgccgcttc acccccctcc ggggcgacct
     1741 ggtcgggctc aaggtcacgg gagtcagtga agtggagccc tcctcccgtt gggtctacga
     1801 cttttccgtg gaagaggacg agaacttcat ctgcggcatg ggcgggatat gctgccacaa
     1861 caccgacgcc gacgtggacg gagcccatat caggacgctg ctcttgacgc tcttctaccg
     1921 cttcatgccc cagctgatcg agaggggcca ccttttcata gcccagccgc ccctctacag
     1981 ggtccaggcc ggcaaggagg tccactactg ctacagcgac aaggacctta aggcgcttca
     2041 ggaccgcctg aagggccgca aactggccat cctgcgctac aagggcctgg gggaaatgaa
     2101 ccccgagcag ctttgggaga ccaccatgga cccggcgacc cggataagca aaagggtcga
     2161 ggtcgaggac gccgtctccg ccgaggagta cttcagcatc ctcatgggcg acaaggtcga
     2221 gccccggcgg gagttcatcg aagcccacgc gaaggaagta aggaacctgg acgtgtagca
     2281 caaggatgag ggcgaaaaga aaaagggtga aggtctaagg aagaaacctc tgagaacgtt
     2341 tgatccaaaa aaatccgtaa gtgaacctgg acgagcagcc ccggaaggag gaaaaaccct
     2401 tccggggctg tgtgttgaac aatgttcaag aactgaaggg gaccttgtcg gaagccccta
     2461 catcgccacg ggttcggcca tgacgaccat cccgtcggca tccaggtaga gcatgaccgt
     2521 ccggcccctg ttctggtcaa ggaaggccca cgggcctatc gccgtgccgc ccctcgacag
     2581 gaagcgcgtc ttttcagggt cccagggata cttctccatc ttcccggtga cgatgtcgta
     2641 gggcagcgtc gtgaggtttt ccaggacagc acccactttg ttctcgtaga tcacctcttc
     2701 ttcc
//
LOCUS       DAOSRG010000163         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34249_length_2698_cov_8.725691, whole genome shotgun sequence.
ACCESSION   DAOSRG010000163 DAOSRG010000000
VERSION     DAOSRG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34249_length_2698_cov_8.725691"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLM30_05560"
     CDS             <1..868
                     /locus_tag="PLM30_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOR54673.1"
                     /translation="RSDALKGADAVITFITQKEMDREELALLKEGCLLQSMQAGVDAL
                     PFDDIPERVIICANTGAWADPMGEHILGMILSLGKHLHKCHDRLSKGIFDRGIINTWF
                     RGKTCGFVGFGGIAKAVARLVRPLGMKVIAVNTSGSTDEEVEFIGTLQDLPRVLEGSD
                     VLVIAVPLTRKTRGLIGQKELALMKDDAILINPARGAIIDQKALYERMKAFPEFRVGI
                     DAWWEEPLVKGEFRLDHPFFELDNLLGSPHNTNLVEGIFPVAAGIAAKNVRIFLEGGE
                     PKNVVDRKDYQF"
     gene            complement(888..>1448)
                     /locus_tag="PLM30_05565"
     CDS             complement(888..>1448)
                     /locus_tag="PLM30_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR54674.1"
                     /translation="LDKAKTILRKGYLGDGSRRESIAEHMWHLALWAMLLRDEIGFKA
                     DLLRTLELVLTHDLVEIYAGDTYAFDEAAVAGQKAREEEAAETLFSSLPNDLAGKFRG
                     LWDEFEEARTPEARFAKALDHLQGFTQNCISKGKSWKENGISRERTFLRTDPPRETDP
                     LFAEILDHLYDVADRENIWGDGQTRN"
     assembly_gap    1449..1548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1549..2457
                     /locus_tag="PLM30_05570"
     CDS             <1549..2457
                     /locus_tag="PLM30_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR54675.1"
                     /translation="GEEVLITQLDHEANRGPWLSLREEGVVVREVALLPEGTLDYDDL
                     AAKINENTRLVAMGFSSNALGTVNDVALARELTHKVGALLLVDAVHYVPHFPVDVKAL
                     GMDFLLCSAYKFYGTHIGILYSREGLLDRVPVQNLRTQEQQAPCRIETGTLNHAAIAG
                     VKSAVEYLSTFGAGNDLRLRIVTGMERIGEYEHILGKEIYLGLREIKGVEVVGPAFTS
                     ERRAPTVSFTMEGMNPIEVCRLLDRRGICAWDGHFYAVRPIEVLGLLEKGGVTRVGVS
                     IYNTTEEVQRLLEAVSDIAGGRLRGD"
     gene            2460..>2698
                     /locus_tag="PLM30_05575"
     CDS             2460..>2698
                     /locus_tag="PLM30_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOR54676.1"
                     /translation="MKVLFIGFGNVGRRMARILTVERSKFPGMKPLDDLVTVGIVTRK
                     SGALISPLGVDLGGALKEMESHGSFNEKNRDFCPLT"
BASE COUNT          536 a          728 c          811 g          523 t
ORIGIN      
        1 ccggtccgac gccttgaaag gggccgacgc ggtcataacg ttcataacgc aaaaagagat
       61 ggaccgggaa gagctcgccc ttcttaagga agggtgcctg ctgcaatcca tgcaggccgg
      121 ggtcgatgcc ctccctttcg acgatatacc cgaaagggtg atcatctgcg ccaataccgg
      181 ggcctgggct gaccccatgg gcgaacatat tctcggcatg atcctctccc tgggcaaaca
      241 ccttcataag tgccacgatc ggctttcgaa aggaattttc gaccgcggca tcatcaatac
      301 ttggttccgg gggaagacct gtggattcgt cggtttcggg ggcatcgcca aggcagtcgc
      361 caggcttgtc cgccccctcg gtatgaaagt gatcgcggtg aataccagcg gcagtaccga
      421 tgaagaggtc gaatttatcg ggaccttgca ggaccttccg agggtactgg agggatcgga
      481 cgtgctggtg atcgccgtcc ccctgaccag gaagaccagg gggctcatcg ggcagaagga
      541 attggccttg atgaaggacg acgcgatcct gataaacccc gcccgcggcg cgatcattga
      601 tcaaaaggct ctctacgaac ggatgaaggc ctttccggag ttccgggtcg ggatcgacgc
      661 ctggtgggaa gagcccctcg tcaagggaga attcaggctg gatcatccct tcttcgaact
      721 ggacaacctg ctcgggtcgc cccataacac caacctcgtg gaggggatct tccccgtagc
      781 ggctgggata gcagcgaaga acgtcaggat cttcctggag gggggagaac cgaagaacgt
      841 tgtggacagg aaggattacc agttctagcg gggaggcacg ttaagtctca attccgcgtc
      901 tggccgtcac cccaaatgtt ttccctgtcg gcgacatcgt agaggtgatc gaggatctcg
      961 gcgaagaggg ggtcggtttc gcggggcggg tcggtcctga gaaaggtcct ctccctggag
     1021 atcccgttct ccttccagga ttttcccttg gaaatgcagt tctgggtgaa accctgcaag
     1081 tggtccaggg ccttggcgaa acgggcctcg ggtgttcgag cctcctcgaa ttcgtcccat
     1141 aggccccgga atttcccggc caggtcgttg gggagggatg agaacagggt ctccgccgcc
     1201 tcttcctccc ttgccttttg ccctgccacg gccgcctcgt cgaaggcgta ggtgtcgccg
     1261 gcgtagatct cgaccaggtc gtgggtcagg accagttcca gggtcctcag gaggtccgcc
     1321 ttgaacccga tctcgtcccg gaggagcatg gcccacaggg ccaggtgcca catgtgttct
     1381 gctatgcttt cacggcggct gccgtcaccg aggtaaccct tcctcaggat ggtcttggcc
     1441 ttgtccagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgaggaagtc
     1561 cttatcaccc aactggacca tgaggccaat aggggtccat ggctctccct aagggaggag
     1621 ggggtggtcg tcagggaggt ggctcttctt cccgagggaa cgctggacta cgatgatctc
     1681 gccgccaaga tcaacgagaa cacgcggctc gtggcgatgg gtttttcttc aaacgctctc
     1741 ggtaccgtca acgatgtggc ccttgccagg gaattgactc acaaggtggg agccctgctc
     1801 cttgtcgatg ccgtccatta cgtcccgcac tttcccgtcg acgtcaaggc cctcggcatg
     1861 gacttcctgc tctgctcggc ttacaagttc tacgggaccc acataggcat cctctactcg
     1921 agggaggggc tccttgaccg cgtacctgtg caaaacctaa ggacccagga gcaacaggcg
     1981 ccctgccgga tcgagacggg caccctcaac cacgcagcca tcgccggcgt caaatcagcg
     2041 gtggaatacc tttccacctt cggcgcgggg aatgatctgc gtttgaggat cgtaaccgga
     2101 atggaaagga tcggcgagta cgaacacatc cttggcaagg agatctacct gggtctaaga
     2161 gaaattaagg gagtagaggt cgtcggtccg gccttcactt cagagaggag ggccccgacg
     2221 gtctccttca ccatggaagg catgaacccc attgaagtat gccggctgct cgaccggagg
     2281 ggtatctgcg cttgggatgg gcacttctac gctgtgcgcc ccatcgaggt cctcggcctt
     2341 ctggaaaagg gcggggtcac aagggtgggg gtatcaatct acaataccac cgaggaagtc
     2401 cagaggctgc tcgaagccgt cagcgatatc gccggaggaa ggctccgggg ggattgaaga
     2461 tgaaggtcct cttcatcggg ttcggcaacg tggggagaag gatggccagg atccttaccg
     2521 ttgagagatc gaaattcccc gggatgaaac cccttgacga tctcgtcacg gtgggaatag
     2581 tcacaaggaa gagcggggcc ctgatctctc ctttgggggt ggacctgggg ggagccctga
     2641 aggagatgga aagccacggc tctttcaatg aaaagaacag ggatttctgc cctctcac
//
LOCUS       DAOSRG010000164         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34394_length_2690_cov_7.403416, whole genome shotgun sequence.
ACCESSION   DAOSRG010000164 DAOSRG010000000
VERSION     DAOSRG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34394_length_2690_cov_7.403416"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLM30_05580"
     CDS             <1..830
                     /locus_tag="PLM30_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54677.1"
                     /translation="SLSWAGQSFQREALGMLTEAVPGWNPSILFVQGREGGIEAHISF
                     SALPPVIIAYSPRISSRTLPRILQTEITDNALEALAPFIGLPGEWVDRHRGPIEAMVA
                     GNLETKWAAREVRGEVTVDITPERIAPVDIRVESDRYTLQAWAAVSLGSDERHPEIGV
                     HLGRMVSPAQGWNLELYGEWVAGTNDLHVESRWGVRWPAWPDIWLGAEVAYPGEDIWF
                     KVWFEELLPRVYLWGRLNDDGDAVAGIGWRFGGYLAWELFYDSRDGDRICVRLVGNL"
     gene            861..1901
                     /gene="lpxD"
                     /locus_tag="PLM30_05585"
     CDS             861..1901
                     /gene="lpxD"
                     /locus_tag="PLM30_05585"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOR54678.1"
                     /translation="MDLSKEITIAELAEILGGRPFGDARRPVRGVQGLGGKDPSRIIV
                     SSDTRAVAATPRGIAIVVPLGGAPNGRTGVEVSDVEKAFAKVLELFSPPRTSMAGVHE
                     SSVIASDAALSPSAFVGPLCLIGAGCVIGDEVVLEAQVHLGSDVSIGARTRIEAGVVL
                     KDGTIVGEDCLIHSGAVIGCDGFGFIPDLEGGHVKIPQLGRVRIGDRVEVGACVTIDR
                     ATLDETVIGDGTVIDDHVHIGHNARIGKNCILVAMTGIAGSAVLEDGVVMAARSGVSD
                     HVTIGERAQVAADGGATKDVPPGATVSGFPARDHREQMKIQALTARLPEMREEIRKLK
                     REVEELKEKSGR"
     gene            1898..>2690
                     /locus_tag="PLM30_05590"
     CDS             1898..>2690
                     /locus_tag="PLM30_05590"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOR54679.1"
                     /translation="MNGVFGLKGEAAFKGVGIHSGAPSEVLVAPFERPGLFFSLGGGV
                     YPVESASADGTARGTTLTFPDGSRVMTVEHLLAALAGLGVWSALLTVSGPEIPAMDGS
                     AGAFAEALIPLREPGLEVTPIAVAREVVFGDPRGGYIGVIPSERFEIACSISYDPSAI
                     GTQLYEGSVSGDVFAEQIAPARTFVLASEIEGIRERGLGLGGSADNVLVIGDCDPTSS
                     LKYRVPSEPVRHKVLDLIGDLATLGRPLKGRVIAHRAGHRMHLELV"
BASE COUNT          475 a          818 c          931 g          466 t
ORIGIN      
        1 gatcactatc ctgggcgggg cagtccttcc agcgggaagc gctggggatg ctcaccgagg
       61 ccgtccccgg atggaacccg tcgatactct tcgtccaggg cagggagggc ggcatcgaag
      121 cccacatcag tttcagcgcc ctcccgccgg tgatcatcgc ctattcgccc aggataagtt
      181 ccaggaccct cccaaggatc cttcagaccg agattaccga caatgccctg gaggccctcg
      241 cccccttcat cggcctcccc ggcgagtggg tcgacaggca caggggcccc atcgaggcga
      301 tggtggccgg taaccttgag acgaagtggg cggcccggga agtcaggggc gaggtcaccg
      361 tcgacatcac ccctgaacgt attgcccccg tagacataag ggtggagagc gaccgataca
      421 ccctccaggc ctgggccgcg gtcagcctgg gctccgacga gagacacccc gagataggag
      481 tccacctcgg aaggatggtc tcacccgccc agggctggaa cctggagctc tacggggaat
      541 gggtggccgg gaccaacgac cttcacgtcg agagccgatg gggtgtaagg tggccggcct
      601 ggcccgatat ctggctgggc gccgaggtgg cgtaccccgg ggaggatatc tggttcaagg
      661 tttggttcga agagctcctg cccagggtct acctctgggg caggctgaac gatgacggcg
      721 acgccgtggc cgggatcggg tggcgcttcg gcggttacct ggcctgggaa ctcttttatg
      781 acagccgtga cggggacagg atctgcgtca ggctcgtcgg gaatctttag cgccgccgcg
      841 gatagaaaag gagaaacagc atggacttaa gcaaggagat caccatcgcc gaactggcgg
      901 agatcctggg cggccgcccc ttcggggacg ctcggcgccc ggtgaggggt gtccagggac
      961 tcggcgggaa ggacccttcc aggatcattg tctccagcga tacgcgagcg gttgcggcga
     1021 ctccgcgagg catcgccatc gtcgtccccc tgggtggcgc accgaacggc cggaccgggg
     1081 tggaggtctc cgacgtggaa aaggcctttg ccaaggtact ggaactattc tcacccccaa
     1141 ggacctccat ggcgggcgtg cacgagtcgt ccgtcattgc ttccgacgcg gccctttctc
     1201 cctcagcttt cgtaggaccc ctttgcctta taggtgcggg ctgcgttatc ggcgatgagg
     1261 tggtgctgga agcccaggtt catctgggct cagatgtatc catcggcgcg aggacccgca
     1321 tcgaggccgg ggttgtcctg aaggacggga cgatcgtggg ggaagactgt ctcatccact
     1381 ccggggcggt gataggctgc gacggcttcg gttttatccc cgatctcgaa gggggtcacg
     1441 tgaagatccc ccaactgggg cgcgtcagga taggcgacag ggtcgaggtc ggggcctgcg
     1501 tcaccatcga cagggcgacc ctcgatgaga cggtgatcgg cgacgggacc gtcatagacg
     1561 accacgtcca tatcgggcac aatgccagga tcgggaaaaa ctgcattctc gtcgcgatga
     1621 cgggcatcgc cggcagcgcc gtcctcgagg acggcgtcgt gatggccgcc cggagcgggg
     1681 tctccgacca tgtcaccatc ggtgagaggg cccaggttgc agccgatggg ggcgctacca
     1741 aggacgtccc tcccggcgcc accgtttcgg ggtttcccgc cagggaccac cgcgagcaga
     1801 tgaagatcca ggcgctgacg gcgaggctgc ccgagatgcg cgaggagatc aggaagttga
     1861 agagggaagt cgaagaactg aaggagaaaa gcggccgatg aacggggtct tcggtttaaa
     1921 gggcgaagcc gccttcaagg gagtggggat ccattcaggg gcaccctcgg aggtcctggt
     1981 ggcacccttc gagaggccgg gattgttctt ctccctcggg ggcggtgttt acccggtcga
     2041 gtcggcgtcg gccgacggca cggcgagggg tacaacactg acctttcccg acgggtccag
     2101 ggtgatgacg gtggagcacc ttctcgccgc cctggcgggc ctcggggtct ggagcgccct
     2161 gttgaccgtg tccgggccgg agatccccgc catggacggg agcgccgggg ccttcgccga
     2221 agccctcatc cccctccgtg agccagggct ggaagtcacc cccatcgccg tggccaggga
     2281 ggtcgtcttt ggtgatcccc gggggggcta cataggcgtg atcccttccg aaaggtttga
     2341 gatcgcctgc tccatcagtt acgatccttc ggccatcggc acccagctct acgaggggag
     2401 cgtcagcggc gacgtttttg ccgagcagat cgcccccgcc aggaccttcg tcctggcgtc
     2461 ggagatcgag ggcatcaggg agcgtgggct cggcctcggc ggctcggccg ataatgtcct
     2521 cgtgatcggc gattgtgacc ctacctcatc tttgaaatac cgggtgcctt cggagccggt
     2581 ccggcacaag gtactggacc tcatcgggga ccttgccacc ctggggaggc cccttaaggg
     2641 gagggtcatc gctcacaggg cgggccaccg gatgcacctt gaactggtcc
//
LOCUS       DAOSRG010000165         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34675_length_2673_cov_9.425898, whole genome shotgun sequence.
ACCESSION   DAOSRG010000165 DAOSRG010000000
VERSION     DAOSRG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34675_length_2673_cov_9.425898"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLM30_05595"
     CDS             complement(<1..398)
                     /locus_tag="PLM30_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR54680.1"
                     /translation="MATTAEIFQRYLEEKARLEPEQVKAVMGDGPLVVVSAGAGTGKT
                     LTLSWRFLRLVVVDGVPLERILTITFTEKASLEMRERIRGLLGEVRDGIPAFSERAGD
                     ALSRLDEAYISTIHAFSMRILRESGLSVDV"
     gene            complement(382..>2673)
                     /locus_tag="PLM30_05600"
     CDS             complement(382..>2673)
                     /locus_tag="PLM30_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR54681.1"
                     /translation="DFAFVGFMSFTRSQLSLIEVLSILGASVRVFMPESGLKIHDARA
                     QLLGDSPAALPTKTSEPIPFLTVVAGDPRLELETLARNLALWRAGKGELQARLGIPFP
                     GWGEIGLSLDAPRLGLAVEVLSRYRIPHTLRGGPSVSETALWKTASSVMEAAALGYPP
                     EETAHILAQPWITPDSFALSDTLSQGPHGKKGWRNHLRSRGDREALSFFERMEAFVSI
                     IEKGAGPSELLRSLGDLAGDAAGVGLSRFIIDHPSLDESARHLNAALEELEHKLEQTA
                     EMENAIGPAGTVILQGSDAAAFLSAWSEHSTVRQPPGMRDSLTLYVGGVPVLAHHKVF
                     ILTGLTADAWPGRLWESPLLDDGRKEAIHGNPETGLSPSHLPLLKEKRSQREAMLLRM
                     IASADEVCLGSRPNQDDPGRPLQSSVFITTALAGPRPWAIEVGGDPPLSRSMKDVIPG
                     EGELAIAGIEARASDPPYRPSRLQEIPPPTPWPEGAAKRVPVSGIDTWGDCPFKYYAE
                     KVLRIEKPRPWGFDPARAGNVAHALWEKAWAERMITGEGLLALARKHWEAALSEQYPE
                     LSLFPARLELFRERAFAMAALQQELDDAGLAAARVFQEREQLLEMEIGGVLFRGKFDR
                     VDLLDDCSALVFDYKTGRSDNLAASLQLPAYAVILQGTREVPVSGYAYLSQRDRSFTG
                     RLEGRARELLPGWGGRSGGKLEAVIEKARERLQAMAESVAASDFPPNYDNDAVCRYCD
                     FQDLCRRKESPRVDRGEENGNDS"
BASE COUNT          417 a          880 c          902 g          474 t
ORIGIN      
        1 tccacgtcga cggagaggcc ggactccctc aggatcctca tcgagaaggc gtggatggtg
       61 gagatgtagg cttcatccag ccgggagagg gcgtcgccag cccgttcgga aaaagcgggg
      121 atcccgtccc tgacctcccc caggagcccg cggatcctct cgcgcatctc cagggaggcc
      181 ttctcggtga aggtgatggt cagtatcctc tccaggggta cgccgtcgac gacgaccagg
      241 cggaggaacc tccaggagag ggtcagcgtc ttccccgtcc cggccccggc gctgacgacc
      301 accaggggcc catcgcccat gacggccttg acctgttccg gctcgaggcg ggctttttcc
      361 tcgaggtacc tctggaagat ctcagctgtc gttgccattt tcctcacccc ggtccacccg
      421 cggggactcc ttcctccggc agaggtcctg gaagtcgcag taccggcaga ccgcgtcgtt
      481 gtcgtagttc ggggggaagt cgctggccgc gacggactcc gccatggcct ggagcctctc
      541 cctggccttc tcgatcacgg cttccagttt gccccctgat cgcccccccc acccgggaag
      601 gagttcacgg gcccggccct cgaggcgtcc cgtgaaggag cggtctctct gggacaggta
      661 ggcgtagccc gagacgggga cctccctcgt tccttggagt atcaccgcgt aggccggcaa
      721 ctggagcgaa gcggccaggt tgtcggacct ccccgtcttg tagtcgaaga ccagggccga
      781 acaatcgtcg aggaggtcga cgcggtcgaa cttcccccgg aagagcaccc cgccgatctc
      841 catctccagc agttgttccc tctcctggaa gacccttgcc gcggcgagcc cggcgtcgtc
      901 cagttcctgc tggagggcgg ccatggcgaa ggctcgctcc ctgaagagtt ccaggcgggc
      961 cgggaagagg gacagttcgg ggtattgttc cgagagggcg gcctcccagt gcttccgcgc
     1021 cagggccagc aggccttccc cggtgatcat cctctccgcc caggccttct cccagagggc
     1081 atgggcgacg ttccccgccc tggcggggtc gaagccccag gggcggggct tttcgatccg
     1141 caggaccttt tcggcgtagt acttgaaggg gcaatccccc caggtgtcaa ttccgctcac
     1201 cgggaccctt ttcgccgccc cctcgggcca gggcgtcggc ggggggatct cctggagcct
     1261 ggaaggcctg tagggtgggt ccgaggcccg ggcctcgatg cccgcgatgg cgagctcacc
     1321 ctcgccgggt atcacgtcct tcatagacct cgagagggga gggtctccgc cgacctcgat
     1381 cgcccaaggc cgcggccccg ccagggccgt cgtgatgaag accgaggact ggaggggtct
     1441 tcccgggtcg tcctggttcg gccgggaccc taggcagacc tcgtcggccg aggcgatcat
     1501 cctaagcagc atagcttccc gctggctcct cttttccttc agcaggggga ggtgggaggg
     1561 cgaaagcccc gtttcggggt ttccgtggat agcctccttg cggccgtcgt ccagcagcgg
     1621 cgactcccag agcctgcccg gccaggcgtc cgccgtgagt ccggtcagga tgaagacctt
     1681 gtggtgggcc agcaccggta cgcccccgac gtaaagggtg aggctgtccc tcatccctgg
     1741 gggctgccgg accgtggaat gttcgctcca ggccgaaagg aacgccgcgg cgtcgctccc
     1801 ctgcaggatg acggtccccg ccggccctat ggcgttttcc atctcggccg tctgctccag
     1861 tttatgctcc agttcctcga gggccgcgtt caggtgcctc gcggactcat cgagggaggg
     1921 gtggtcgatg atgaagcggg agaggccgac cccggcggca tcgccggcga ggtctcccag
     1981 tgaacggagg agttccgagg gtccggcccc cttttcgatg atcgaaacga aggcctccat
     2041 cctttcaaaa aacgaaaggg cttcccggtc tccccggctc cgaagatggt tcctccaccc
     2101 cttctttcca tggggccctt ggctgagggt gtccgagagg gcgaaactat cgggggttat
     2161 ccagggctgg gccaggatat gggccgtttc ctccgggggg tagcccaggg cggcggcctc
     2221 catgacggaa gaggcggtct tccaaagggc cgtctccgac acgctgggcc ctccccggag
     2281 ggtatggggg atccggtagc gggagaggac ctccacggcg agccccagcc tgggggcgtc
     2341 cagtgaaagc cctatctcgc cccacccggg gaaggggatg ccgagcctgg cctggagttc
     2401 acccttgccg gccctccaaa gggcgaggtt acgggcgagg gtctccagtt ccagtcgggg
     2461 gtccccggcg acgacggtta gaaaaggtat gggttcggag gtctttgtcg gaagcgcggc
     2521 ggggctgtct ccgaggagtt gggccctggc atcatggatc ttcagcccgc tctcgggcat
     2581 gaagaccctg acgctcgctc ccagtatcga gagcacctcg atgaggctca actgggagcg
     2641 ggtgaaactc atgaagccca cgaaggcgaa gtc
//
LOCUS       DAOSRG010000166         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_34870_length_2663_cov_5.764187, whole genome shotgun sequence.
ACCESSION   DAOSRG010000166 DAOSRG010000000
VERSION     DAOSRG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34870_length_2663_cov_5.764187"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2663)
                     /locus_tag="PLM30_05605"
     CDS             complement(<1..>2663)
                     /locus_tag="PLM30_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR54682.1"
                     /translation="SVKRPVMTLMVFLAVLVIGSISFLNLKLDLLPKIEPPVMTVITP
                     WPGASASDVEQRITKVMENNLSMLEGVDDLISRSLDNISVVSVKFKWGVDLDVRAGDV
                     RDAVNFAKRELPSDAEESVVLRITSGTVPVVEVAMTAERSYQGLYHFVDKDVAEELSR
                     VPGVGQVLIFGGVGREIQVLLDADRLEAIGLPPDAIAGVLERENLNIPAGSMKEGRTE
                     YFIRVPGRFTSVEEIADTVVGIRNGNPVRLADVAEVRDGYKELTMNGWKDGQAAVILI
                     VMKNSDANTIEVSRSVLERLARLRDESFPSDVTYEVVLDTADFIMNSIINLSVSLMAG
                     IILVFLVTWAFLRRFRASLIVAGAIPFSLILTFIAMGRLDYTINIFTLSALAMASGMV
                     VDNAIVATDQIIYHIEQGSRRRVAAVLGASEIGSALLASTLTTIVVLFPLAFLSGLVG
                     VFFSALTVVMVLAVSASLFVALTFIPMMGSKFFGREPGKLKIHQVTERFFSRMEEMYS
                     SMLAWGLDNRKKVIASSLLLLLLTFLGFRTIGTELTPDPDTGDISITFSLPEGTRLEV
                     TDELVREVMDYCRRTIPEAKLVFGMDGMEEGGLSVAVGQKAGPNTGTIGIKLVDKNKR
                     DRSAFEIAGEIRDWIHAKPGIEDMTVFVSSPIKAMFLGSKPLDIEIYGDDLRQVTKAS
                     SMIASGLAEIPGTADISVSRREDRPELWIEPDREKAAHLGVSTAAVSRTARIYFAGYE
                     TEGSFWEGEDDFPIRVRLREDQRNSRDVFGRLTVPSSSGALVRLSSVATMKDEVGPPQ
                     VERKNRQRYLTVGANVHGRSLGEVTADTMKMVQAMEIPEGVRVTFGGQIKEQQDAFRQ
                     MGLLVLLGIMLVFMVMAGQYEAYLDPFVILF"
BASE COUNT          507 a          831 c          815 g          510 t
ORIGIN      
        1 gaagagtatg acgaagggat ccaggtaggc ctcgtactgg cccgccatga ccatgaagac
       61 gagcattatg cccagcagga ccaggagccc catctgccgg aaggcgtcct gctgctcctt
      121 tatctggccc ccgaaggtaa ccctcacgcc ctcggggatc tccatggcct ggaccatctt
      181 catagtgtcg gcggtgacct ccccaagaga ccgtccgtgg acgttggccc ccacggtcag
      241 gtatctctgc cggtttttgc gctccacctg gggcggaccc acctcgtcct tcatcgtcgc
      301 caccgacgac agccgtacga gcgctcccga tgacgaggga acggtgagcc gcccgaacac
      361 gtccctgcta tttctctgat cttccctcaa cctcaccctg atggggaagt catcctcccc
      421 ctcccagaag gagccctcgg tctcgtaacc ggcgaagtag atcctggcgg tgcgggagac
      481 ggcggcggtg ctgacgccca ggtgggccgc cttctccctg tccggttcga tccagagttc
      541 gggccgatcc tcccggcggc tgaccgatat atcggctgta cccggtatct cggcgaggcc
      601 cgaggctatc atggacgacg ccttcgtcac ctgcctgagg tcatcgccgt agatctctat
      661 atccaggggc ttggaaccca ggaacatggc ctttatgggc gaactgacga agacggtcat
      721 gtcctcgatg ccaggcttcg cgtggatcca gtcgcggatt tcgccggcaa tctcgaaggc
      781 ggacctgtcc ctcttgttct tgtcgacgag ctttatgccg atagtacccg tgttggggcc
      841 cgctttctgg ccgacggcca cggagagccc gccttcctcc attccgtcca tcccgaagac
      901 gagtttggcc tcggggatgg tccgccggca gtagtccatg acctccctga caagttcgtc
      961 ggtgacctcg agcctggttc cctcgggaag cgagaaagtt atagatatgt cgcccgtgtc
     1021 cgggtcgggg gtcagttcgg tcccgatggt ccggaaaccg aggaaagtga gcagcagcaa
     1081 caggagcgac gaggctatga ctttcttgcg attgtccagc ccccaggcca acatggacga
     1141 atacatttcc tccatgcggg agaagaatct ctcggtcacc tggtgtattt tcagtttccc
     1201 cggctcccgg ccgaagaatt tgcttcccat catggggatg aaggtgagag ccacgaaaag
     1261 agacgccgag accgccagga ccatgaccac cgtgagggcc gagaagaaga cgcccaccag
     1321 gccggaaagg aaagcgaggg ggaaaagcac gacgatcgtg gtgagcgtcg aggccagcag
     1381 ggccgaaccg atctcgctgg cgccgaggac ggccgcgacg cgccgccggc tgccctgttc
     1441 tatgtggtag atgatctggt ccgtggcgac gatggcgttg tccaccacca tgccgctggc
     1501 catggccagg gccgagaggg tgaagatgtt tatcgtgtag tcgaggcgcc ccatggcgat
     1561 gaaggtaagg atcagcgaga agggtatggc ccccgccact atcagggagg cgcggaatct
     1621 tctcaggaag gcccaggtca cgaggaagac caggattatc cccgccatga gggagacgct
     1681 gaggttgatg atggagttca tgatgaagtc ggccgtgtcc aggaccacct cgtaggtcac
     1741 gtcggagggg aagctctcat ctctgagccg ggcgagcctc tccaggaccg accgggagac
     1801 ctcgatggtg ttggcgtcgc tgttcttcat cactatgagg atgacggcgg cctggccgtc
     1861 cttccacccg ttcatggtca attccttgta accgtccctt acctcggcga catcggccaa
     1921 cctgacgggg ttgccgttac ggatgcccac gacggtgtcc gcgatctctt ccaccgaggt
     1981 gaacctgccc ggcaccctta tgaagtactc cgtccggccc tccttcatgg acccggcagg
     2041 gatgttgagg ttctccctct cgagaacgcc cgcaatggca tcggggggca gcccgatggc
     2101 ctccagcctg tcggcgtcga gaaggacctg gatctcccgg cccacgcccc cgaagataag
     2161 gacctggccc actcccggca ccctggagag ttcctccgcc acgtccttgt cgacgaagtg
     2221 gtagagcccc tggtaggacc tttcggccgt catggcgacc tccacgacgg ggaccgtccc
     2281 cgaggttatc ctgagcacca ccgattcctc cgcgtcggag gggagttccc tcttggcgaa
     2341 gttgacggca tccctcacgt cgccggcccg gacatcgaga tccacgcccc atttgaactt
     2401 caccgagacg acggagatgt tgtcgaggga cctggagatg aggtcgtcca ccccttcaag
     2461 catggagaga ttattctcca taaccttggt tatcctctgc tccacgtcgc tggccgacgc
     2521 ccccggccac ggggtgatca ccgtcatgac gggcggctcg atcttcggga ggaggtccag
     2581 tttaaggttg aggaaggaaa tagaacctat gaccaggacg gcgaggaaga ccatcagggt
     2641 catcacgggg cgcttgacgg aga
//
LOCUS       DAOSRG010000167         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35019_length_2655_cov_8.239231, whole genome shotgun sequence.
ACCESSION   DAOSRG010000167 DAOSRG010000000
VERSION     DAOSRG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35019_length_2655_cov_8.239231"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLM30_05610"
     CDS             <1..766
                     /locus_tag="PLM30_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54683.1"
                     /translation="TGNPWLGVVAAFLTGVAISLVHAFLSVSVGANQVVSGLAVAMFG
                     TGASALLGRKMVGLTITGFGKIPVPVLSGIPIAGPVFFDHDPLVYISYGLAFFLYWFI
                     FHTRPGLNLRAVGESPIVVDAMGLSAARLRYVYTLVGGGLAAVGGSYLSLAYTKMWSE
                     GMSAGRGWIAVALVIFALWNPVRAILGAYLFGGVGAMQLRIQAAGTSFPAPILMMMPY
                     IFTILVLLVISVKKGKGILLGSPGALGVPYSREERT"
     gene            complement(768..1277)
                     /gene="gpt"
                     /locus_tag="PLM30_05615"
     CDS             complement(768..1277)
                     /gene="gpt"
                     /locus_tag="PLM30_05615"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOR54684.1"
                     /translation="MKEIDSRYKKTYPVSWSQLHNDCRALAWRLLELRTDWERIIAIA
                     RGGLVPAAIIARELDIHFIDTVCVSSYTVRAQGGFKVIKNVAGDSERTLIIDDLVDTG
                     KTARIVREMLPLSHFATVYAKPDGRPLTDTYVTEVSQETWILFPWDSEPQYQYIDPIA
                     DLSRNGRKG"
     gene            complement(1318..>2655)
                     /gene="cysS"
                     /locus_tag="PLM30_05620"
     CDS             complement(1318..>2655)
                     /gene="cysS"
                     /locus_tag="PLM30_05620"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR54685.1"
                     /translation="KVSFYSCGPTVYDFFHIGNARPFIIFDVLRRYLEAKGFEVSYVQ
                     NFTDIDDKMINRAAQLGITVRELADRFIEAYYEDADALGVKRPTFSPRATEFIPEIIG
                     LVRRLVERGHGYVVDGDVYFDVKSFPGYGKLAKQSLEDLLSGARIEVDPRKKHPLDFA
                     LWKGKKEGEPSWESPWGEGRPGWHIECSAMAMALLGETIDIHAGGSDLVFPHHENEIA
                     QAEAATGKPFVRYWIHNGYILMDQEKMSKSLGNILTAREARQRFSPSAIRLFMLSAHY
                     RSPISFSDESLGQAESAVARIRNSYHELVSAPVGGDGSSQEGEALLLDEALERFNAAL
                     DDDLNTAGAIGVLFEAVRAINQATSGGKNLAPAFREKALSFFDHVDGILGLIEMTEEG
                     DKRLEEEVEALLAERERARAERDFKRADSIREELASRGILLEDTPHGTRWRRS"
BASE COUNT          414 a          797 c          854 g          590 t
ORIGIN      
        1 cacgggcaac ccgtggctgg gtgtcgtcgc agccttcctc acgggtgtgg ctatatcgct
       61 ggtgcacgcc tttctttccg tctccgtcgg cgcgaaccag gtcgtgagcg gcctggccgt
      121 cgccatgttc gggaccggcg ccagcgccct cctgggcagg aaaatggtcg gtctcaccat
      181 aaccggtttc gggaagatcc ccgtgccggt gctctcgggg atccccatcg cgggcccggt
      241 gttcttcgac cacgaccccc tggtctacat ctcctacggg ctggctttct tcctttactg
      301 gttcatcttt cacaccaggc ccggcttgaa cctgagggcc gtgggggagt cgcccatagt
      361 cgtcgacgcc atggggctca gcgccgcacg gttgcgttac gtctataccc tggtcggggg
      421 tgggctggcg gccgtgggcg ggagttacct ctccctggcc tacacgaaga tgtggagcga
      481 ggggatgagc gccggtcgcg gctggatcgc cgtggcgctg gtgatctttg ccctctggaa
      541 tcccgtcagg gcgatcctgg gcgcctacct tttcgggggc gttggggcga tgcagctgag
      601 gatacaggcg gcgggaacca gttttcccgc cccgatcctg atgatgatgc cctacatttt
      661 caccatcctt gtcctgctgg tcatatcggt gaaaaagggc aaggggatcc tcctgggctc
      721 cccaggggcc cttggggtgc cctattcgcg ggaggagagg acctgattca ccctttgcgg
      781 ccgttccttg aaaggtccgc tatggggtcg atgtactggt actggggttc ggagtcccag
      841 gggaagagta tccaggtttc ctggctcacc tcggtgacgt aggtgtcggt gaggggcctc
      901 ccgtcgggct tggcgtagac cgtggcgaaa tggctcaggg ggagcatctc cctgacgatc
      961 ctggcggtct tgcccgtgtc gaccaggtcg tcgatgatca gggtcctctc tgagtcgccc
     1021 gctacgttct tgatcacctt gaacccgccc tgcgccctga cggtgtaact ggagacgcag
     1081 acggtgtcga tgaagtggat gtccagttcc ctggcgatga tggcggccgg gaccaggccg
     1141 ccccttgcga tggctatgat cctctcccag tcggtcctca gttccagcaa cctccaggcc
     1201 agggccctgc agtcgttgtg gagctggctc caggatacgg gataggtttt cttgtagcgg
     1261 ctgtcgatct ccttcatgct cgtccttccc cgggcgggat gatcatggcc ggtcctttca
     1321 ggaccgtctc caacgggtcc catggggggt atcctcgaga aggatgcccc tggaagccag
     1381 ttcttccctt atggaatcgg cccttttgaa gtccctttcg gcccgggccc tttccctttc
     1441 agccagaagt gcctccacct cttcctcgag tctcttgtcg ccctcttcgg tcatttcaat
     1501 gagacccagt atgccgtcga cgtgatcgaa gaaggaaagg gctttttccc tgaaggcggg
     1561 cgcgaggttt ttaccgcccg acgtggcctg gttgatggcc ctgacggctt cgaaaaggac
     1621 tccgatggcg ccggcggtgt tgaggtcatc gtcgagggct gcgttgaaac gctccagcgc
     1681 ctcgtcgagc agaagtgcct ccccttcctg ggagctcccg tccccgccca cgggcgcgct
     1741 gaccagttcg tggtaggagt tccttatcct ggctaccgcg ctttcggcct gccccaggct
     1801 ttcatcggaa aaacttatgg gggacctgta gtgggcgctg agcatgaaga gcctgatggc
     1861 tgagggggag aaccgctggc gggcctcgcg ggccgtgagt atgttgccca gggacttgga
     1921 catcttttcc tggtccatga ggatgtagcc gttatggatc cagtacctca cgaagggttt
     1981 gcccgtggct gcctcggcct gggctatctc gttctcgtgg tgggggaaga ccaggtcgct
     2041 ccccccggcg tggatatcga tggtctcgcc gaggagggcc atggccatgg cgctgcattc
     2101 tatgtgccac cccggacggc cctcgcccca agggctctcc caggaaggct caccttcctt
     2161 tttacccttc cagagggcga agtccagggg gtgctttttt ctggggtcca cctcgatgcg
     2221 ggcccccgaa aggaggtctt ccagggactg cttcgccagc ttgccgtagc cgggaaagga
     2281 cttgacatcg aagtagacgt cgccgtccac aacgtatccg tgaccccttt cgacgaggcg
     2341 cctcaccagg ccgatgatct cggggatgaa ctcggtggcc ctgggagaaa aggtgggacg
     2401 cttcacgccg agggcgtcgg cgtcctcgta gtaggcctcg atgaaccggt cggccagttc
     2461 ccggacggtg atgccaagct gggccgccct gtttatcatc ttgtcgtcga tgtcggtgaa
     2521 gttctggacg taggacacct cgaacccctt cgcctccagg taacggcgga ggacgtcaaa
     2581 gattatgaag gggcgggcgt tgccgatatg gaagaaatcg tataccgtag ggccgcagga
     2641 gtaaaaactg acctt
//
LOCUS       DAOSRG010000168         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35080_length_2651_cov_6.436826, whole genome shotgun sequence.
ACCESSION   DAOSRG010000168 DAOSRG010000000
VERSION     DAOSRG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35080_length_2651_cov_6.436826"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLM30_05625"
     CDS             complement(<1..952)
                     /locus_tag="PLM30_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR54686.1"
                     /translation="MFEALKKTLGLDRNERTLKRYATAVARINDLEASMRSLPEDELA
                     GLGPLFRQRAMKGEALNEMLPEVFAAVREASRRTLGLRHFDVQLMGGMALHEGKIAEM
                     KTGEGKTLVATLAVALNALKGEGVHLVTVNDYLAKRDAEWMGPIYRYLGLSVGVIYTF
                     MDQGDRMAAYRADVTYGTNSEFGFDYLRDNMAILASQLVQREHSYIILDEVDSILIDE
                     ARTPLIISGPSEENVEVYRKADDVARRLHKGEDFELEEKERNVALTEVGIAKCEKILG
                     LPDLFTDYQRSELGHRIVQALKAHHLFQRDVHYVNKDGEIV"
     gene            complement(1120..1662)
                     /gene="pgsA"
                     /locus_tag="PLM30_05630"
     CDS             complement(1120..1662)
                     /gene="pgsA"
                     /locus_tag="PLM30_05630"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOR54687.1"
                     /translation="MNIWNLPNILSLSRVFLAPLVMVFLTLRGQFGNILGLPFGDILA
                     GLVFTIAALTDTFDGYFARKHGMVTNLGKIIDPLADKILVIAALISLVQLQRLPAWIV
                     VIILSREFFVTGLRMVAASEGMVISASRGGKVKTVTQVIAILLMIFDLPGGLPAMWVA
                     LVATLWSGMEYLAKAKSLLS"
     gene            complement(1728..2432)
                     /locus_tag="PLM30_05635"
     CDS             complement(1728..2432)
                     /locus_tag="PLM30_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR54688.1"
                     /translation="MLRIKDLHVHYGGIHAIKGISLEVPRGKVVTLIGANGAGKSSTL
                     RAIAGIVKKSSGSIELEGREISGLAPEDIVKSGIMMAPEGRKILPQLTVLENLMLGAF
                     SRNDPEGIDRDLAWVYELFPRLKERAWQKGGTLSGGEQQMLAVGRALMGSPSLLMLDE
                     PSLGLAPILVKDVFDIIQAINNEGKTVLLVEQNAYAALKIADAAYVLEVGRIVLSGTG
                     KQLLNDPKVREAYLGG"
     gene            complement(2425..>2651)
                     /gene="livG"
                     /locus_tag="PLM30_05640"
     CDS             complement(2425..>2651)
                     /gene="livG"
                     /locus_tag="PLM30_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163479.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HOR54689.1"
                     /translation="NETQELMGFVKDIRTRFKLTILLIEHDMRVVMGICEHIWVLDYG
                     VTIAEGRPEEIRSNQKVIEAYLGEEYVRDA"
BASE COUNT          511 a          804 c          768 g          568 t
ORIGIN      
        1 tcactatctc gccgtccttg ttgacgtaat ggacgtccct ctggaacagg tggtgagcct
       61 tcagtgcctg gacgatcctg tgaccgagct cggacctctg gtagtcggtg aaaaggtcgg
      121 gcaggccgag aatcttttcg cacttggcta tgccgacctc ggtgagggcc acgttccgtt
      181 ccttctcctc cagttcgaag tcctctccct tgtggagcct cctggcgacg tcgtcggcct
      241 tcctgtagac ctcaacattt tcttcggagg gacccgagat tatgaggggc gtccgggcct
      301 cgtcgatgag gatggagtca acctcgtcga ggattatgta tgaatgctcc cgctggacca
      361 actgggaggc gaggatggcc atattatccc tgaggtagtc aaagccgaat tcgctgttgg
      421 taccgtaggt cacgtcggcc ctgtaggccg ccatcctgtc gccctggtcc atgaaggtgt
      481 atatcacgcc cacggatagc cccaagtacc tgtagatcgg ccccatccac tcggcatccc
      541 gcttggccag gtagtcgttg accgtgacca ggtgcacccc ttcgcctttc agggcgttca
      601 aggccacggc cagggtcgcc accagggtct tgccctcgcc ggtcttcatc tcggcgatct
      661 tgccctcgtg gagggccatc cctcccataa gctgcacatc gaaatgtcga agtcccaggg
      721 ttctcctcga cgcctcccga actgcggcaa agacctcggg gagcatttca ttgagggctt
      781 cgcctttcat ggccctctgc ctgaaaagag gccccagccc ggcgagttca tcctcgggaa
      841 gggacctcat cgacgcttcc aggtcattta ttctggccac ggccgtggcg tagcgtttga
      901 gggtcctttc gttccggtcg agccccaggg tcttcttcaa agcttcaaac attcacttca
      961 cctcgatcgg cgtcaaagag gggagcgctc ctcaaagtga cattatattc cccctggtaa
     1021 ggagcgtcaa aaaaggagag gcccccaggg cctccagcca aagggaggct ttgatgaagg
     1081 gacgccacgg agggggtccc ctgaagagtg aaaggaaggt taggaaagaa ggcttttagc
     1141 cttggccagg tactccatcc cggaccacag cgtggcgacc agggcgaccc acatggccgg
     1201 gagacccccg gggaggtcga agatcatgag gaggatggct atgacctggg taacggtctt
     1261 gaccttcccc cccctcgacg ccgagatcac catcccctcc gaggcggcta ccattcggag
     1321 accggtcacg aagaactccc tggaaaggat gatgaccact atccaggccg gcagccgctg
     1381 gagctggaca agagagatca gggcggcgat cacgagtatt ttgtcagcca ggggatcgat
     1441 gatcttgccc aggttggtga ccatgccatg cttcctggcg aagtacccgt caaaggtatc
     1501 ggtgagagcc gctatggtga agaccaggcc ggcgagtatg tcaccgaagg ggagacccag
     1561 gatattgccg aactgacccc tgagtgtgag gaagaccatg acaagggggg ctagaaagac
     1621 tctggagagg ctcagtatgt tgggcagatt ccagatattc agggctcacg cacctccagc
     1681 gccgcgttta gggggggccc caccccgtgg gggggacccc cttcacccta ccctcccagg
     1741 taggcttcac ggaccttcgg gtcattcaga agttgcttcc ctgtacccga gagcactatc
     1801 cttccgactt ccaggacgta ggccgcgtcg gcgatcttta gagcggcata ggcgttctgc
     1861 tcgaccagca ggaccgtctt cccctcgttg tttattgcct ggattatatc gaagacatcc
     1921 ttaaccagga taggcgccag ccccagcgaa ggctcatcga gcataagaag gctgggcgat
     1981 cccataaggg ccctgccgac ggcgagcatc tgctgctccc cgccggagag ggtacctccc
     2041 ttttgccacg ccctctcctt gagcctgggg aagagttcgt atacccaggc gaggtcacgg
     2101 tcgatcccct ccgggtcgtt cctgctgaag gcgccgagca taaggttctc caggacggtg
     2161 agttggggga ggatcttcct cccctccggc gccatcatta tccccgactt gactatgtcc
     2221 tccggggcaa ggccggagat ctccctgccc tcgagctcta tggaaccgct gctttttttg
     2281 acgatcccgg cgatagcccg gagggtggaa cttttccccg cgccgttggc cccgatgagg
     2341 gtgacgacct tgcccctggg cacttccagg gagatcccct tgatggcatg tatcccgccg
     2401 taatggacgt ggaggtcctt tatcctaagc atcgcggacg tactcctctc caaggtaggc
     2461 ctcgatgacc ttctgattgg atcggatctc ctcgggccgg ccctcggcta tggtcacgcc
     2521 gtaatcgagg acccatatgt gttcgcagat acccatcact accctcatgt catgttctat
     2581 cagcagtatg gtcagtttga acctggtcct gatatccttg acgaaaccca tcagttcctg
     2641 ggtctcgttg g
//
LOCUS       DAOSRG010000169         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35213_length_2644_cov_5.506373, whole genome shotgun sequence.
ACCESSION   DAOSRG010000169 DAOSRG010000000
VERSION     DAOSRG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35213_length_2644_cov_5.506373"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLM30_05645"
     CDS             <1..401
                     /locus_tag="PLM30_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR54690.1"
                     /translation="GDDVNIQDRDVIISGGKGMKREENFRLLRELAELLDGGVGASRA
                     AVDNKWIAYSHQVGLSGKVVAPKAYFAVGISGSVQHLAGMQTSGLVVAINKDPEAPIF
                     RVADIGLCGDLFDIVPRLIEGLRERKGVSG"
     gene            398..1816
                     /locus_tag="PLM30_05650"
     CDS             398..1816
                     /locus_tag="PLM30_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOR54691.1"
                     /translation="MTHYSAVTEEVVEDLKSLVGPHNVVVEPEKLASYSRDEVALQFW
                     DREYRAEVLVLPESTDHVSAVLAYADPRMIPVTPRGAGTGLSGGAVPAFGGIVMAFDR
                     MNRILEVDLENLTLTAEPGVVTAEIQAAAARHGLEYAGDPCSGDASFIGGNVAENAGG
                     NKVIKYGTTGANVLALEVVLPSGKVTWFGGKRRKDVTGLDLVHLMVGSEGILGVITKV
                     VLKLVPRHEKVVDLLVPFPTVEEAITLVPRVMRELKITPSSLEFMDRNSLRIVSRYTG
                     STLPFQEAGAHLIIQIEGNDMDALSDQYEDIGDFCLENGALEVFVADNRNFRDLLWKT
                     RKNIAEAIIVFYTKYAKEDLVVPTGNIPSLLKRVDEVCRKYRLEAVNFGHVGDGNIHV
                     NLLAGEESENWQERIEGARRDLYRSTRELGGTLSGEHGVGLKRKDFLGMFLDEAQVEL
                     IRGIKRTFDPNLILNPGKIVDI"
     gene            1934..2548
                     /locus_tag="PLM30_05655"
     CDS             1934..2548
                     /locus_tag="PLM30_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54692.1"
                     /translation="MKGRTPLFVALTFLFILFAQGALGDQEWYERPLSPGEGPSEGPR
                     WEGWFFDGEGRWLREAVAPGEGDVNRFFADVYGDWYSLGLDSTGAIQPVDPPDWYPWD
                     DTPERRGLPPKGKDEVLSGESRYSFRMGEWENFFGAWFRRIDPPGLPPLGVVHLIDSK
                     GDCFVLVVYPGASPRPVKRFGWFTGFPPGWGIKPGTTVLVELEQ"
BASE COUNT          545 a          768 c          843 g          488 t
ORIGIN      
        1 ccggggacga tgtcaacatc caggaccggg atgtgatcat ctccggcggc aagggcatga
       61 agagggagga gaatttcagg cttctcaggg aactggcgga actgctcgac gggggcgtgg
      121 gggcaagccg ggcggcggtg gacaacaagt ggatagccta ctcccaccag gtcggcctca
      181 gcgggaaagt ggtcgccccc aaagcctact tcgccgtggg catatcggga tcggtccagc
      241 acctcgccgg catgcagacc tcgggcctcg tggtggccat aaacaaggac ccggaagccc
      301 cgatcttccg cgtggcggat atcggtctct gcggcgacct tttcgatatc gtgccccgcc
      361 tgattgaagg cctcagggag aggaagggtg tctcgggatg actcattact cggccgtcac
      421 tgaagaggtc gtcgaggacc tgaaaagcct ggtggggccc cataacgtgg tagtggaacc
      481 ggagaagctc gcctcctatt cccgcgatga ggtcgccctc cagttctggg acagggaata
      541 ccgcgccgaa gtgctggtcc tcccggagag tacggaccat gtatcggcgg tcctggccta
      601 tgccgatccc cggatgatac ccgttacgcc caggggtgca ggaacgggcc tctcgggtgg
      661 ggcggtcccg gccttcgggg gcatcgtcat ggccttcgac aggatgaacc gcatcctgga
      721 agttgacctg gaaaacctta ccctcaccgc cgagcccggc gtggtgaccg ccgagatcca
      781 ggcggccgcg gccaggcacg gccttgagta tgccggggac ccctgcagcg gcgacgcctc
      841 cttcatcggg gggaacgtcg cggagaacgc gggaggtaac aaggtaataa aatacggtac
      901 cacgggagcc aacgtgcttg ccctcgaagt ggtcctgccc tcggggaagg tgacctggtt
      961 cggcggcaag aggcgcaagg acgtcaccgg cctggacctg gtccacctca tggttggttc
     1021 ggagggcatc ctgggagtaa taaccaaggt ggtgctcaaa ctcgttcccc ggcatgaaaa
     1081 agtggtcgac ctcctcgtcc ccttcccgac cgtcgaagag gctataaccc tggtcccacg
     1141 ggtcatgagg gaactgaaaa taaccccctc gtccctcgag ttcatggaca ggaactccct
     1201 ccgcatcgtt tccaggtaca ccgggagtac gctccccttc caggaggccg gggcccacct
     1261 gatcatccag atcgagggca acgatatgga cgccctgtcg gaccagtacg aggacatcgg
     1321 cgacttttgc ctggagaacg gtgccctgga agttttcgtg gcggacaacc gtaatttccg
     1381 cgacctcctg tggaaaaccc gcaaaaatat cgccgaggcg atcatcgtct tttacacgaa
     1441 atacgcgaag gaagacctgg tggtccccac gggcaatata ccctccctcc ttaaaagggt
     1501 cgatgaggtt tgtcgaaaat accggcttga ggccgtcaac ttcggccacg tcggcgacgg
     1561 gaacatccac gtcaacctcc tggcgggtga agagagcgaa aactggcaag agcggatcga
     1621 aggggccagg agggatcttt atcgatcgac cagggaactc ggcggaaccc tttcaggtga
     1681 gcacggcgtg gggcttaagc ggaaagactt cctcgggatg ttcctggacg aagcccaggt
     1741 ggagttgata cggggaataa aaaggacctt cgaccccaac ctcatcctca acccgggcaa
     1801 gatcgtcgat atctgaaggt acggcagccc ggtaagtcag gaccaaggcg gaaataattc
     1861 cgttccccct ctggtatgat acgggcgtct cgcgaggttc atttcacagc tccgatgagg
     1921 aggtgtgccc ctgatgaaag gcaggactcc ccttttcgtg gccctcactt ttttgttcat
     1981 cctgttcgcc caaggggccc tgggcgacca ggaatggtac gaacggcccc tttccccagg
     2041 ggaaggcccg agtgaagggc cccgttggga gggctggttc tttgacggcg aggggcggtg
     2101 gctcagggag gcggtcgccc ccggtgaggg agacgtcaac aggttcttcg ccgatgtcta
     2161 cggtgattgg tacagcctgg gcctggactc caccggggcc atccagccgg tggacccacc
     2221 cgattggtac ccatgggatg acacgcctga aagaaggggt cttcccccga agggcaagga
     2281 cgaggtcctc tccggcgagt caaggtattc cttccggatg ggggaatggg aaaacttttt
     2341 tggagcctgg ttccgaagga tagacccccc cggtctcccg cccctgggtg tggtccacct
     2401 tatcgacagc aagggcgact gctttgtcct ggtggtttac ccgggagctt ccccgaggcc
     2461 cgtcaagagg ttcggctggt tcaccggttt tccccccggc tgggggataa aacccgggac
     2521 gacggtgctg gtggagttgg aacagtaagc ctgccgccga cttgacaaat cccttcggat
     2581 accctattgt tcgccacggt ggcaaagcca ccgaaaacac tttctggcaa cagaagaaat
     2641 ggga
//
LOCUS       DAOSRG010000170         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35555_length_2625_cov_9.784436, whole genome shotgun sequence.
ACCESSION   DAOSRG010000170 DAOSRG010000000
VERSION     DAOSRG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35555_length_2625_cov_9.784436"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..91)
                     /locus_tag="PLM30_05660"
     tRNA            complement(4..91)
                     /locus_tag="PLM30_05660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ser,seq:tga)
     gene            182..571
                     /locus_tag="PLM30_05665"
     CDS             182..571
                     /locus_tag="PLM30_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54693.1"
                     /translation="MKLIRAAVLPMILATALLLVSGAAFAQGGCGVPGELKVVLTGAE
                     WEFDLKTGRLSGEAVVKNVCDGDVVAPGILVGIYGVNGEMVNNLAQRGTEMRLAPGKS
                     VKVKFRIDMNEAPASLMFTPFEGMTST"
     gene            732..1148
                     /locus_tag="PLM30_05670"
     CDS             732..1148
                     /locus_tag="PLM30_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR54694.1"
                     /translation="MKMEEIIEKYVCSPSTVAVAGASPKEERAVHGVMEYLKEEGFRL
                     FPVNPSYAGEDIQGLPCLGSITEVGEKFDILGLFISPDRQQPVLEDLDKLGYKPIIWM
                     QPGAENDVAEAALKARGYEVVKGACLMMVHEVYCEK"
     gene            1273..2448
                     /gene="nifS"
                     /locus_tag="PLM30_05675"
     CDS             1273..2448
                     /gene="nifS"
                     /locus_tag="PLM30_05675"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOR54695.1"
                     /translation="MTERQVYMDHSATTAVDRGVLEAMLPFFSEEYGNPNSLHAWGRR
                     ARKAVNEAREKVAALIGSDASEVIFTGGGSEADNLAIKGSTSMRKDKGRHVITTAIEH
                     HAVMDAFGWLDKQGFDVTVLPVDDKALVSVTELEKAIRPDTILFSMMFANNEVGTIQP
                     VAEAAKICRDRGILFHVDGVQAAGHVPFNVRDLGVDMLTMSAHKMYGPKGVGGLYLRK
                     GVRVEPIVHGGGQERGLRSGTENTAGIVGFGAAAELAVRRLADGSIDEEARLRDLLIK
                     RVTSSIEEAFLTGHPTQRLPFLASFCIGSLEGESMLLRLDAAGIGASSGSACTSGSLE
                     PSHVLLAMGLAHEVAHGSLRFSLGKDTTEEDIVYVAENLERIVADLRALSPLWKTRG"
     gene            2453..>2625
                     /locus_tag="PLM30_05680"
     CDS             2453..>2625
                     /locus_tag="PLM30_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOR54696.1"
                     /translation="MYTEKVMELFMNPKNAGRMEDPDGVGEVGNPRCGDIMKIYIKVK
                     DDRIADISFETFG"
BASE COUNT          544 a          762 c          853 g          466 t
ORIGIN      
        1 aactggcgga gggcagagga ttcgaacccc tggagacttg cgcccctgct gatttcaagt
       61 cagccgcctt cgaccgctcg gccagccctc cgacggcaat caattataat gaattctgct
      121 agaataagcc aacgaactgc cgggtgcgta tcgagactga acggaagcgg aggtgtcatc
      181 gatgaagttg atccgtgccg ctgttttacc gatgatactg gccacggctc ttttgctggt
      241 atcgggcgcc gcctttgccc aggggggttg cggggtgccg ggcgaactga aggtcgtact
      301 gacgggggcg gaatgggagt tcgacctaaa gacggggcgt ctttccggcg aggccgtcgt
      361 gaagaacgtc tgcgacggcg atgtcgtcgc cccgggcatt ttggtgggca tctacggggt
      421 taatggtgag atggtgaaca acctggccca gaggggcacc gagatgaggc ttgccccggg
      481 caagtccgtc aaggtgaagt tccgtatcga tatgaatgag gccccggcct cgctcatgtt
      541 cacgcccttc gagggcatga cgagcacctg aggctgattt tccgccccgc gtcgcgggga
      601 ttgttcaaaa gacgtacggt gtccagcgcg atatccggga ttcgagggcg gtggcggcct
      661 gtttcttgcc cgccggatgg ccaccgcctg ccggcgtttc acggtcctgg caccggaagg
      721 aggcttaccc catgaaaatg gaagagatca tagaaaagta cgtctgctcc ccctccacgg
      781 tggccgtagc cggcgcctcg cccaaggagg agcgggccgt ccacggcgtc atggagtact
      841 tgaaagagga aggcttcagg ctcttccctg tcaacccttc ttacgccgga gaggatatcc
      901 agggcctccc ctgccttggc tccataacgg aggtcggcga gaagttcgac atactggggc
      961 ttttcatatc ccccgaccgg cagcagccag tgctggagga cctcgataag ctgggataca
     1021 aacccataat atggatgcag cccggggccg agaacgacgt ggccgaggcc gccctgaagg
     1081 cccgaggtta cgaggtggtg aagggtgcct gcctgatgat ggtccacgag gtctactgcg
     1141 agaaatgaag aaaaaagcgg cccttgccga gaaaggaaaa aacgcgggag gtattgacct
     1201 tcggccgaaa gagttgtatc ctcttcatgg aagttaggtt agcctaataa aaagagatcc
     1261 gaggcgatac caatgacaga aagacaggtc tacatggacc attcggccac gacggccgtc
     1321 gaccggggcg tactcgaagc gatgctcccc ttcttttccg aggagtacgg caacccgaac
     1381 agcctccacg cctgggggag aagggcccgg aaggccgtca acgaggccag ggagaaggtc
     1441 gccgccctga tcgggtccga cgcctccgaa gtgatattca ccggcggggg gagcgaggcc
     1501 gacaacctcg ccatcaaggg aagcacgtca atgcgcaaag acaaggggcg ccatgtgata
     1561 accaccgcca tcgagcacca cgcggtgatg gacgccttcg ggtggctcga caagcagggt
     1621 ttcgacgtca ccgttctgcc cgtggacgat aaggccctgg tcagtgtgac ggagctggaa
     1681 aaggcgatca ggcccgacac gatccttttc tccatgatgt tcgccaacaa cgaggtcggc
     1741 acgatccagc ccgtggccga ggcggcgaag atatgccgcg acaggggcat ccttttccac
     1801 gtcgacgggg tccaagccgc cggtcatgtg ccctttaacg tccgggacct gggcgtcgat
     1861 atgctgacca tgtcggccca caagatgtac ggtcccaagg gagtaggggg cctctacctc
     1921 cgcaagggcg tcagggtcga gccgatcgtg cacggcgggg gacaggagcg gggcctcagg
     1981 tccggcaccg agaacaccgc cggtatcgtg ggcttcgggg ccgcggccga actggccgtg
     2041 aggcgccttg ccgacggctc catcgacgag gaggcccgcc tccgcgatct gctcatcaaa
     2101 agggttacct cttcgataga agaagccttc ctgacggggc accccacgca gaggctgccc
     2161 ttcctggcca gtttctgcat aggttccctc gagggggaat cgatgctcct gcgcctcgat
     2221 gccgccggga tcggcgcttc cagcgggtcc gcctgcactt cggggagcct cgagccgagc
     2281 cacgtcctcc tcgccatggg cctcgcccac gaggtggccc acggttcgct ccgcttctcg
     2341 ctgggcaagg ataccaccga agaggatata gtctacgtgg cggagaacct ggagaggatc
     2401 gtcgccgacc tcagggcctt gtctccgcta tggaaaacca gggggtgaag ccatgtacac
     2461 cgaaaaagtg atggaactct tcatgaaccc caagaacgcc ggccggatgg aggatcccga
     2521 cggcgtgggg gaggtgggca acccccggtg cggggatatc atgaagatct atatcaaggt
     2581 caaggacgac cggatcgccg atatttcctt cgagaccttc ggctg
//
LOCUS       DAOSRG010000171         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35577_length_2624_cov_6.596730, whole genome shotgun sequence.
ACCESSION   DAOSRG010000171 DAOSRG010000000
VERSION     DAOSRG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35577_length_2624_cov_6.596730"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLM30_05685"
     CDS             <1..569
                     /locus_tag="PLM30_05685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR54697.1"
                     /translation="EADVSAPEAVQSAFREIEARLGGVDILVSNAGISVRSGFSDITF
                     EQWSKVLEVNLGGMFLCAREASKRMSSGGVILMTASTNGMEGHPFYADYNASKAGVIL
                     LAKTMALEFAPLIRVNSVSPGYVLTPMQRAEYTPEMLETVNARIPFRRHADPSEVAAL
                     FAFLASDQASYITGANIPIDGGETAGLQ"
     gene            588..1355
                     /locus_tag="PLM30_05690"
     CDS             588..1355
                     /locus_tag="PLM30_05690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR54698.1"
                     /translation="MDDDSIFRLDGKVAVITGGSSGIGLGCARFLSRMGAAIVLADID
                     EPGGRAVESDLGRNCLFVKCDVTRDEDCRNMATVVMARFGRVDILVNCAGIIKRKDIV
                     ELAEKEWDLTMAVTLKGVYLVSRHLIPFMARGCGGSIVNIASGWGVKGGPKAVSYCAA
                     KGGVVNMTRAMAIDQGPQGIRVNSVSPGDTDTPLLRDEARQLGIDEKKWLEESADRPL
                     KRLGTPEDIAMAVYFLVSGLSPWVTGANLVVDGGGTA"
     gene            1382..>2624
                     /gene="gcvPA"
                     /locus_tag="PLM30_05695"
     CDS             1382..>2624
                     /gene="gcvPA"
                     /locus_tag="PLM30_05695"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOR54699.1"
                     /translation="MKGKKRVYPYIPNSEPRVREAMLRDIGVDSVDDLLGDIPEEIRI
                     KSSMNLPEPFPAEADLKRHVEGLLSRNNTVGECLSFLGAGCYDHHVPSVVDEVIGRSE
                     FLTAYAGEPYEDHGRFQVLFEYQSMMAELLDFDVCNVPTYDGAQAAGTSLRMASRLTG
                     RKKALLAGNMNPDRLKVVRTYLDPVMTVEMFGWDRSSGLLDLEDLGSKMSEEVAAVYF
                     ENPSYLGTIEDQGERIARAAHKAGALMVVFADPSTLGVLEPPSRYGADIACGDIQPLG
                     VHMNYGGGLGGFICTKGENRFIEEYPSRLFGIAPTREKEWGFGDVAWERTSFANRETA
                     KEFVGTHAALWGIAAAVYLASMGPGGMADLGKVILQRSLYARKVLGEVPGVSTGKPSG
                     ACFKEFVVDFGQSGRDVADINE"
BASE COUNT          537 a          751 c          868 g          468 t
ORIGIN      
        1 ttgaagccga tgtctcggcc cccgaagcgg tgcagtcggc gttcagggag atcgaagccc
       61 gcctcggggg tgtggacatc ctcgtatcca acgccgggat tagtgtcagg agtggtttca
      121 gcgacataac cttcgaacag tggagcaagg tcctggaggt caacctgggc ggcatgttcc
      181 tctgcgccag ggaggcttca aagcggatgt cctcgggtgg ggtgatactg atgacggctt
      241 cgaccaacgg gatggaaggt caccccttct acgcggacta caacgcctcg aaggcaggag
      301 tcatcctcct cgccaagacc atggccctgg agttcgcgcc gctgataagg gtcaactccg
      361 tgtcccccgg gtacgtcctt acacccatgc agagggcgga atatacaccg gagatgctcg
      421 aaacggtcaa cgcgaggatc cccttcagac ggcacgcgga cccttcggag gtcgccgccc
      481 ttttcgcctt cctggcctcc gaccaggcat cctatatcac cggggccaac atacccatcg
      541 acggcggcga gaccgccgga ctccaataag aaagggagga acggggattg gacgacgaca
      601 gcattttcag gcttgatggc aaggtagccg tgataaccgg gggtagttcg ggtatcggct
      661 tgggttgcgc ccgttttctt tcccgcatgg gcgccgcgat cgtcctggcg gatattgacg
      721 agccaggggg gcgagcggtt gagagcgacc tgggaaggaa ttgccttttc gtgaagtgcg
      781 acgtgaccag ggatgaagat tgcaggaaca tggccaccgt ggtgatggcc cggttcggcc
      841 gggtggatat cctggtcaac tgcgccggca tcatcaagag aaaggacata gtggaactgg
      901 ccgaaaagga atgggacctc acgatggccg tcacgctgaa gggcgtctat ctggtctcga
      961 ggcatctcat acccttcatg gccaggggct gcggcggcag catcgtaaac atagcctcgg
     1021 gctggggggt aaaagggggc cccaaggccg tctcctactg cgccgccaag ggcggggtgg
     1081 tgaacatgac gagggccatg gccatcgatc aaggccccca ggggatccgg gtcaactccg
     1141 tctcgccggg cgacaccgac acgcctcttc tccgcgatga ggccaggcaa ctgggcatcg
     1201 acgaaaaaaa gtggctcgag gagagcgccg acaggccgct gaaaagactc gggacccctg
     1261 aggatatcgc catggccgtc tattttcttg tgagcggcct ctccccgtgg gtcacggggg
     1321 ccaaccttgt cgtcgacggc ggcgggacgg catgaaaagc cggagcagga ggtaattagc
     1381 catgaagggc aagaaaagag tttaccccta tattcccaat tcggagcccc gcgtcaggga
     1441 ggccatgctc agggatatcg gcgttgacag cgtcgatgat cttctcggcg acatccccga
     1501 agagatcagg ataaagtcca gcatgaacct tcccgaaccg ttcccggccg aggctgacct
     1561 gaagcggcac gtggaagggc tcctgtccag gaacaacacc gtcggggaat gcctcagttt
     1621 ccttggggcg gggtgttacg atcaccacgt cccgtccgtg gtcgacgaag tcatcgggag
     1681 gtcggaattc ctgacggcct acgccgggga accctacgag gatcacggcc gtttccaggt
     1741 cctcttcgaa taccagagca tgatggcgga attgctcgac ttcgatgtat gcaatgttcc
     1801 gacctatgac ggcgcccagg ccgccgggac atcgctgagg atggcctccc gattgacggg
     1861 caggaaaaag gccctgctgg cgggcaacat gaaccccgac aggctcaagg tggtgcggac
     1921 ctacctcgac ccggtgatga cggtcgaaat gttcggttgg gaccgatcct ccgggttact
     1981 ggacctcgag gacctggggt cgaagatgag cgaagaagtg gccgcggtct atttcgagaa
     2041 cccctcctac ctggggacga tcgaggacca gggcgagagg atcgcccggg cagcccacaa
     2101 agccggggcc ctcatggtgg tcttcgcgga cccctccacg ctgggcgtcc tggagccccc
     2161 ctccaggtat ggggccgata tagcctgtgg ggacatccag cccctggggg tccacatgaa
     2221 ctacggcggg ggcctgggtg ggttcatctg caccaagggc gagaaccgtt ttatagagga
     2281 gtacccctcc cgcctatttg ggatagcccc gacccgggaa aaagagtggg gcttcgggga
     2341 tgtggcctgg gagaggacct ccttcgccaa cagggagacg gcgaaggaat ttgtcggcac
     2401 ccatgccgcc ctctggggca tcgcggcggc ggtctacctc gcttccatgg gacctggggg
     2461 catggccgac ctgggaaagg tcattctgca gcgatcccta tacgccagga aggttctggg
     2521 tgaagtgccg ggcgtgtcga cgggaaaacc ctcgggagcc tgcttcaagg agttcgtcgt
     2581 cgatttcgga cagtccgggc gcgatgtggc cgacataaac gaaa
//
LOCUS       DAOSRG010000172         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_35835_length_2610_cov_10.843444, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000172 DAOSRG010000000
VERSION     DAOSRG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35835_length_2610_cov_10.843444"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLM30_05700"
     CDS             complement(<1..845)
                     /locus_tag="PLM30_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR54700.1"
                     /translation="MREYFRLDLLAVLTAGVLWGTLSPAGKQLSLLGADMLTVAVLRA
                     AFMAGGVALFLLARMPHRFRIGGRQLLQVAILAGPCMVGIYAGFFFALHHLSVPVTIV
                     IFFTHPLLTTIGSLVITKELPDRRQVAGALLTFVGVAVGVLSSEGSLSGGIDPVGIAW
                     CLFAALGMSLYSLFGRLSAQTGFIPQATLFFYLQFFGLGWLFLLKTITAGWGDLPSIT
                     TAQLLWIMYVGLGGGMLGYSIYFYGLRTLPASTASIVSSIEIVTAFTLSAVLLGQPPA
                     SREVVG"
     gene            933..1436
                     /locus_tag="PLM30_05705"
     CDS             933..1436
                     /locus_tag="PLM30_05705"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOR54701.1"
                     /translation="MAEQRGFPFVSGPGPLLLILGSFPGKRSLQERQYYAHPRNAFWT
                     IMGGLFKAGPELPYEDRLERLRGARVALWDVLLYCERPGSMDGAIIADGVIPNDFGRF
                     FQDHPTVTAIFFNGRKAAELYERFVLPDLDADKRALRRQTLPSTSPAYASKSLAQKMN
                     DWGILLG"
     gene            complement(1537..2400)
                     /gene="panB"
                     /locus_tag="PLM30_05710"
     CDS             complement(1537..2400)
                     /gene="panB"
                     /locus_tag="PLM30_05710"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOR54702.1"
                     /translation="MAKVTIQQLQKMKEAGEKISMVTAYDYSQAVLVEKAGIEVILVG
                     DSLAMTTLGHEGTTALTTDEMIAHIKPVVKGAPSPLVIGDLVFGSYNESLSQAVHSST
                     RLIKEGGCDAVKLEGAHPDVIKAIVDGGIAVQGHLGLTPQTAVLLGGFKVQGKGKEAG
                     LQLLDDAKRLEEAGVFSIVLECVPEELGRVMSGLVDVPVIGIGAGRYCDGQVLVLHDM
                     MGLFDRFTPRFVKQYRQIGKEMVEAFMEFKAEIRNGSFPSEENVFTGFSREEADALFE
                     ELKSHGVHQDR"
BASE COUNT          594 a          778 c          720 g          518 t
ORIGIN      
        1 cccactacct ccctggaggc cgggggttga cccaacagga cggccgaaag ggtgaaagcc
       61 gttactatct ctatggatga gacaatgctc gccgtcgacg ccggcaaggt cctgaggccg
      121 taaaaataga tactgtagcc cagcatgcct ccaccaagcc caacgtacat gatccataac
      181 agttgtgccg tggtgatcga gggaagatcg ccccacccgg cggtgattgt cttgagcagg
      241 aagagccagc ccaggccaaa aaactgcaaa tagaaaaaca gcgtggcttg gggtatgaat
      301 ccagtctggg ctgaaagtct tccaaaaagc gaatacaagg acatgcctag ggcggcgaaa
      361 agacaccagg cgataccaac ggggtctatc cctcccgaaa gggacccctc cgatgaaagc
      421 acccctaccg ccacacccac gaaagtaagc aacgccccgg cgacctggcg gcgatcgggg
      481 agctccttcg ttatcaccag cgaaccgatg gtcgtgagca ggggatgggt gaagaaaatg
      541 acaattgtga ccggcaccga gaggtgatga agcgcgaaga agaacccggc gtagatgccc
      601 accatgcagg gtccggcaag gatcgcgacc tgtagtagtt ggcggcctcc gattcggaag
      661 cggtgcggca tgcgtgccag gaggaaaaga gccactcccc cggccatgaa agccgccctc
      721 aagaccgcca cagtgagcat gtcggcgccc aaaaggctca gctgtttacc agcggggctc
      781 agggtgcccc agaggacgcc cgccgtgagg acggctaaaa ggtcaagtcg gaagtattct
      841 ctcaaagtgt cgtaccctct tcagtaggtt tgaaatgctt ttgccttttg cttgcgtcat
      901 ccttattata aaggtacggg agtgatggat cgttggcaga acagagaggt ttcccctttg
      961 tgtccgggcc gggaccgctt ttgctcatcc tgggcagttt cccggggaaa cggtcgctcc
     1021 aggagaggca atattacgct caccccagga acgctttctg gacgatcatg ggcggactct
     1081 tcaaagccgg accggaactc ccttatgaag acaggcttga aaggctccgc ggcgcgaggg
     1141 ttgccttgtg ggacgtcctg ctctactgcg agaggcccgg aagcatggac ggcgcgatca
     1201 tcgctgacgg agtcatccca aacgactttg gacggttttt tcaagaccat ccgacggtca
     1261 cggcgatctt tttcaacggc cgcaaggctg cggaactata tgaacgcttc gtcctgcctg
     1321 acctggacgc cgacaaaagg gccttgcgca gacagacact tccatcgacc agcccggcct
     1381 acgcctcgaa aagccttgcc cagaagatga acgactgggg aatattactg ggatagggct
     1441 cgacacaacc ttgacccgcc ttcccgggcc ggacctcgaa aaaaccggcc aacgcaaagg
     1501 acacctgggc attgccaact cccaggctcc tttcggtcaa cggtcctgat gcaccccgtg
     1561 ggacttgagt tcctcgaaaa gagcatcggc ctcttccctg gagaatccag tgaatacatt
     1621 ctcttccgac gggaaggaac cgttccttat ttccgccttg aattccatga aggcctccac
     1681 catttctttc cctatctgcc tgtactgctt gacgaacctg ggcgtgaacc ggtcgaaaag
     1741 ccccatcatg tcgtgcagca ccagaacctg cccgtcgcaa tacctgcctg cgccaatgcc
     1801 gatgaccggc acatccacaa ggccggacat gaccctcccc aattcttcgg ggacacattc
     1861 cagcacgatg gagaaaactc ccgcctcctc caacctcttc gcatcatcga gcaactggag
     1921 cccggcttct ttgcccttcc cctggacctt gaaccctccc aggaggacgg cggtctgggg
     1981 tgtgagcccc aggtgcccct ggacggctat acctccatca acgatggcct tgatgacgtc
     2041 ggggtgcgcg ccctcgagtt ttacggcatc gcacccgccc tccttgatga gccgggttga
     2101 cgaatgaacc gcctgggaaa gactctcgtt gtaagaaccg aaaacgagat cccctatgac
     2161 caggggtgaa ggtgccccct tcactacggg cttgatatgg gcgatcattt catcggtggt
     2221 tagggccgtc gtcccttcgt ggcccagggt ggtcatggcg agagaatcgc ccaccaggat
     2281 cacctcaatg ccggctttct ccaccaggac ggcctgggaa taatcgtagg ccgtcaccat
     2341 ggagatcttc tcacctgcct ccttcatctt ctgcagttgc tggatggtca ctttcgccac
     2401 gggtctcaca accttttcga gaatatttta ggcctttttc tgtctcgatc tagtacctgg
     2461 gtgtttcaaa tatatacgaa cagggattaa ggggccagag ctgggcgtga agaacaaaac
     2521 cagggccaca ggggtagacc ctttcgggaa catctgccct gtctctgccc gggcgctcct
     2581 ggaaagccaa catcttttta ggaggtcgtc
//
LOCUS       DAOSRG010000173         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_36382_length_2579_cov_13.368859, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000173 DAOSRG010000000
VERSION     DAOSRG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36382_length_2579_cov_13.368859"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLM30_05715"
     CDS             complement(<1..738)
                     /locus_tag="PLM30_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOR54703.1"
                     /translation="MGKTMIEKIIRRAAGREVKVGERVWCDIDLSTARDFGGANCVLQ
                     FEEVTDKKGKVWNPDKVAFTFDLQAPSHSEKVSNNQKIIREFAKKQGIKKVFDVDWGI
                     GQHVLLENGLVKPGDVILGTDSHMNLLGGVGAFATGVGNTDIVASWFEGTNWFRVPET
                     MKVTVTGKFKKGVCMRDLLTHIVGTLGADGMFFLATEFYGETIENSSLAERITLCSMV
                     TEMSGKVGLIMPNGEVLDWLVGRAGDEV"
     gene            complement(773..1327)
                     /locus_tag="PLM30_05720"
     CDS             complement(773..1327)
                     /locus_tag="PLM30_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR54704.1"
                     /translation="MRDRIVKVFPEIDWVQDKGLREKVIDTMEDALKTGGWEPEDMEK
                     IPFTLLIPDCPASLNVHNRGVTRMAKAIYDEYNPLYEKNGGFKLDHDTLIAGAILHDV
                     GKLVEYEKKDGKIVKSQMGKDLRHPFSGVGLAMRNGIPTRICHCIAVHAKEGDGGYRC
                     PEAVVINKCDFINFETIKSFLGLL"
     gene            complement(1375..2073)
                     /locus_tag="PLM30_05725"
     CDS             complement(1375..2073)
                     /locus_tag="PLM30_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOR54705.1"
                     /translation="MIEKRLRHTRIYEQVVEELKGLIASGELLPGDPLPPERQLMAEM
                     GVSRSSLREAFRVMELLGLIDSVPGKGRFVRKPRGEAADSRNMPLEDEAVLELMEARR
                     VLDPAIAGAAAMHAMPADLMKLRKVLSRTEKNLEDIEKRSQDDFDFHLALSEATRNFV
                     FINIVKMTFNLIMATHERIYSLLSDKEAFLNEHRAIYEAITDHDVAAAQALAAGHIER
                     IYKTLQEALALEVK"
BASE COUNT          440 a          785 c          760 g          594 t
ORIGIN      
        1 gacctcgtcg ccggccctgc cgaccagcca gtccaggacc tcgccgttgg gcatgatgag
       61 ccccaccttg ccgctcatct cggtgaccat ggagcagagg gttatcctct cggccaggga
      121 cgagttctcg atggtctcac cgtagaactc ggtggccagg aagaacatcc cgtcggcgcc
      181 gagggtcccc actatgtggg tcagcaggtc gcgcatgcat acgccctttt tgaacttgcc
      241 cgtgacggtg accttcatcg tctcgggcac gcggaaccag ttggtcccct cgaaccagga
      301 ggccacgatg tcggtgttcc ccacgccggt ggcgaaggcg cccaccccgc cgaggaggtt
      361 catgtggctg tcggtcccca ggatcacgtc gccgggtttc acgaggccgt tctcgagcag
      421 gacgtgctgg ccgatgcccc agtccacgtc gaagaccttc ttgatgccct gcttcttggc
      481 gaattcacgg atgatcttct ggttgttgga aaccttttcc gaatgggacg gggcctggag
      541 gtcgaaggtg aaggccacct tgtcggggtt ccagaccttg ccctttttgt ccgtcacttc
      601 ctcgaactgc agcacgcagt tggccccgcc gaagtcgcgg gccgtcgaca ggtcgatatc
      661 gcaccagacc ctctcgccga ccttgacctc ccgaccggcc gcccgccgga tgatcttctc
      721 gatcatcgtt ttgcccatgg gtcatgcacc tcctgtttgt tggtcgtcct tgctagagaa
      781 gccccaggaa cgacttgatg gtctcgaagt tgatgaaatc acacttgttg atgaccaccg
      841 cctcggggca acggtaaccg ccgtcgccct ccttggcgtg gacggcgatg cagtggcata
      901 tcctggtggg gatgccgttc cgcatggcca ggccgacgcc ggagaagggg tgccggaggt
      961 ccttgcccat ctgggacttc acgatcttgc cgtccttctt ctcgtactcc accagtttgc
     1021 ccacgtcgtg gaggatggcg ccggcgatga gtgtgtcgtg gtcgagtttg aaaccgccgt
     1081 tcttctcgta gagggggttg tactcatcgt agatggcctt ggccatcctc gtcacgcccc
     1141 ggttgtggac gttaagggaa gcggggcagt cggggatgag gagggtgaag ggtatcttct
     1201 ccatatcctc gggttcccaa ccgccggtct tgagggcgtc ctccatggtg tcgatcacct
     1261 tttccctgag ccccttgtcc tggacccagt cgatctccgg gaaaaccttc acgatcctgt
     1321 ctctcatgct aaatccctcc aatgggtgtt ttgaaattct ggcgcctcta tcaattactt
     1381 gacctcaagg gccagcgctt cctgcagggt tttgtagatc ctctcgatgt gacccgcggc
     1441 cagcgcctgg gcggcggcca cgtcgtggtc ggtgatcgcc tcgtagatgg cccggtgttc
     1501 gttgaggaaa gcctccttgt cggagaggag cgagtagatc ctctcgtggg tggccatgat
     1561 caggttgaag gtcatcttca cgatgtttat gaagacgaaa ttccgggtcg cctcggagag
     1621 cgccaggtga aagtcgaaat cgtcctggga gcgcttctcg atatcctcca ggttcttctc
     1681 ggtcctggag aggactttcc gcagtttcat caggtccgcg ggcatcgcgt gcatcgccgc
     1741 ggccccggcg atggccgggt cgagcaccct gcgggcctcc atcagttcga gcacggcctc
     1801 gtcctcgagg ggcatgttcc gcgaatcggc cgcttcgccc ctgggcttcc gtacgaagcg
     1861 acccttaccg gggacgctgt cgatgagccc cagcagttcc atgaccctga aggcctccct
     1921 gagggaactc ctgctcacgc ccatctcggc catgagctgc cgctcggggg ggagcgggtc
     1981 ccccggcagg agctcaccgg aggcgatgag gcccttgagt tcctctacca cctgctcgta
     2041 tatcctggtg tggcggagtc ttttttcgat catttctcga cctccccggt ggaccaccca
     2101 gtacacttta tataccaggt agaccttttg tgcaagagtt cgcaataaga ctaaatgttc
     2161 agacttcata aacgatttcc accgggagcg gcgaggattg catacaaaag tttgacagct
     2221 ccctttcgtt caactattat tgcccggtcc tgggggcgca gaatccaaat tttaatgttt
     2281 cattgggatc cttcggctcg tttcgcgact cttcccgggg accatcggtg gacctttcga
     2341 tcggtattct cctttcacct tccccttcac cccctgtgga gagcacgcgc cttatccggc
     2401 ccaagggctg gtatcagtcg gagactgcgt cactggaagg acagttcggc gtgcgtcaat
     2461 caaaggaggg attctgtcat gaaagcagga cgaaaaggcg cattgatcct ggcactggtt
     2521 cttgccgtcg tgacggggct gacctcagtt cccgccgcaa tcgcagccga aagggtagt
//
LOCUS       DAOSRG010000174         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_36452_length_2576_cov_12.930980, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000174 DAOSRG010000000
VERSION     DAOSRG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36452_length_2576_cov_12.930980"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLM30_05730"
     CDS             complement(<1..1049)
                     /locus_tag="PLM30_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54706.1"
                     /translation="MKKVNSRIVFTAALLALMVTASGAAWGAMGEVTSFAFRAAQEDV
                     LGLERSLEKDGKADTHFALSLKGIGAITDVSLKALDGAREWSPAGGGGRWAMVVKDAS
                     GKTVSSESGLPVTPFLGFANLNLFVADDGTAFSETREYEVTVKFIDGSTASAKTEVKG
                     MPDIFGTPKSPEPESGVMTAVLYGIGDRDVAGKRETLGKDGTNDAHFQVRFTTLSAVD
                     QVTIRNVDGTNAVWDTVPGNGIWAIAVFQDGKLKNRADGSVRFTVDGDTSLDLWVADN
                     GAIAGGKTRFEVIVRFEDGTTFSETALPGTPEVTSPVEDILSAVLYAPATTDIANRGE
                     TVGANGTPDWRITLSL"
     gene            complement(1131..1643)
                     /locus_tag="PLM30_05735"
     CDS             complement(1131..1643)
                     /locus_tag="PLM30_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOR54707.1"
                     /translation="MAERIAVLVENDFHDIEFWYPFYRLQEAGYEPIIVAPAAPKVYR
                     GKYGTTVEAPYDAGSVAAQSPAGVVIPGGWAPDRLRMSAEIVGLVRGTYESGGFVAGI
                     CHAGSLLVSCGILAGKRVTSYPSLRDDMILAGAEWVDAEVVVSDRIITSRRPSDLPAF
                     MAEVMKALAT"
     gene            1842..2180
                     /locus_tag="PLM30_05740"
     CDS             1842..2180
                     /locus_tag="PLM30_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR54708.1"
                     /translation="MKITSFREAAQVTTPHGVKVCKLYDTPDAQVVHITLEPGESLKK
                     HITPVDVFFYVLEGTGVVEIGDEKKAVSKDALIESPKDIPHCWYNESGAAFRVLVVKT
                     PRPVASTRLL"
     gene            2227..>2576
                     /locus_tag="PLM30_05745"
     CDS             2227..>2576
                     /locus_tag="PLM30_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR54709.1"
                     /translation="MVFEIKKDVFWVGVKDWALNFFHGHELSTHRGSTYNAYLVRDKK
                     IAVVDTVWDPFTEKYLDNVKSLVDPSRIDYVVANHAETDHSGALGALMELAPQAEVIV
                     SPRGMDSIPGHFHK"
BASE COUNT          477 a          828 c          787 g          484 t
ORIGIN      
        1 agggagaggg tgatcctcca gtcgggagtc ccgttggcgc cgacggtctc gccccggttg
       61 gctatgtccg tcgtcgcggg ggcgtagagc accgctgaaa ggatatcctc cacgggcgat
      121 gtcacttcgg gggtccccgg gagggccgtc tcgctgaagg tggtcccgtc ctcgaacctg
      181 acaatgacct cgaagcgggt cttgcctccg gctattgccc cgttatcggc cacccagagg
      241 tccagcgagg tgtccccgtc cacggtgaag cgcacgctcc cgtcggcccg gttcttgagt
      301 ttgccgtcct ggaacacggc tatcgcccag atcccgttgc cggggaccgt atcccagacc
      361 gcgttggtcc catccacgtt gcgtatcgtt acctggtcca ccgccgagag ggtggtgaag
      421 cgtacctgga agtgggcgtc gttggtgccg tccttgccca gggtctccct cttgccggcc
      481 acgtcccggt caccgatgcc gtaaaggacc gccgtcatga ccccgctttc gggctccggc
      541 gacttggggg tcccgaagat gtcgggcatc cccttgacct cggtcttggc cgaggcggtg
      601 ctcccgtcga tgaacttgac ggtgacctcg tattcccggg tctcggaaaa ggcagtcccg
      661 tcgtcggcga cgaaaaggtt caggttggcg aaccccagga agggcgtcac gggtagcccc
      721 gactcggaag agaccgtctt ccccgaggcg tccttgacga ccatggccca gcggccgccc
      781 cccccggcgg gggaccattc cctcgcaccg tcgagggcct tcagcgagac atcagtgatg
      841 gcgccgatgc ccttgaggga aagagcgaaa tgggtgtcgg ccttcccgtc cttttccagg
      901 gacctttcga gacccaggac atcctcctgg gcggccctga aggcgaagga agtcacctca
      961 cccatggctc cccaggcggc tcccgaggcc gttaccatca aggccagcaa ggcggccgtg
     1021 aaaactatcc tgctgttcac ctttttcata ctccgtccct ccccgttctt ctgcggccca
     1081 aagtcaacct gaaaacatta tagcatcgtc gacccggtac cgggcgggtt tcaggtcgcc
     1141 agggccttca tcacctcggc catgaaggcc gggaggtccg acggcctcct gctggttatg
     1201 atcctgtcgg agaccaccac ctcggcatcc acccattcgg ctcccgccag gatcatgtcg
     1261 tccctgaggc tggggtagct cgtcaccctc ttcccggcga gtatcccgca ggagaccagc
     1321 aggctgccgg cgtgacagat gcccgccacg aaaccgcccg actcgtaggt ccccctgacg
     1381 agtcccacga tctcggcgga catcctgagc cggtccggcg cccacccgcc gggtatcacc
     1441 acaccggccg gcgattgggc cgcgacactc ccggcgtcgt agggggcttc caccgtcgtg
     1501 ccgtactttc cccggtagac cttgggggct gccggagcga cgatgatggg ctcatacccc
     1561 gcttcctgca gcctgtagaa gggataccag aactctatgt cgtggaaatc gttctccacc
     1621 aggacagcta tcctttccgc catttccggg ctcctctcgt ggggatggcc ttcactcccc
     1681 ctgatgatcc gaattataca gccttgctcc ggataagtgt ttaaatagct tttagggtat
     1741 tattctaaag gtaccccgat gacttgagtg cgatggaggg ggacctgcgg aaagggggtc
     1801 atgggaaaag ggaggtcccc attaaaagga gggatgatcc tttgaagata acgagcttca
     1861 gggaagccgc gcaggtcacg accccccacg gggtcaaggt ctgcaagctc tacgatacgc
     1921 ccgacgcgca ggtggtgcat ataaccctgg aaccggggga gtcgctgaaa aagcacatca
     1981 cccccgtgga cgtgttcttt tacgtcctcg agggcaccgg cgtcgtggag atcggtgacg
     2041 agaagaaagc cgtgtctaag gacgcgctta tagaaagccc caaggacata cctcactgct
     2101 ggtacaatga gagcggggcc gccttcaggg tgctggtggt gaagacgccg aggcccgtcg
     2161 cctcgacgag attgctttga gggcgggggt gcgccacccc gccgaaaagg aaaggagaga
     2221 tgcgaattgg tcttcgagat caagaaggat gttttctggg taggggtcaa ggactgggcc
     2281 ctcaactttt tccacggcca tgaactttcg acccaccggg ggagcaccta caacgcctac
     2341 ctggtccgcg acaaaaagat agccgtggtg gacacggtct gggacccctt cacggagaag
     2401 tacctggaca acgtcaagtc cctggtcgat ccctccagga tcgactacgt ggtggccaac
     2461 cacgccgaga ccgaccactc cggggccctg ggcgccctga tggaacttgc cccccaggcc
     2521 gaggtgatcg tctccccccg gggcatggac agcatcccgg gccacttcca caagga
//
LOCUS       DAOSRG010000175         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_36703_length_2564_cov_11.259466, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000175 DAOSRG010000000
VERSION     DAOSRG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36703_length_2564_cov_11.259466"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLM30_05750"
     CDS             <1..576
                     /locus_tag="PLM30_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside reductase class II"
                     /protein_id="HOR54710.1"
                     /translation="WKTHCKGITVFRDGCRSGQVLYVDKPAVQQALPIPQVSEVESPM
                     ERPFILGGKTAKIPTSYGNLYLTVNEVNGRPVEVFTTMGKSGHETMAFTEAMGRLISL
                     SLRSGVRLHSIIKQMKGIGGSQPVWHGDGVIMSVPDAIAYGLLSMGYLDMEEKGMMES
                     LSDTDMCPVCGASMTRQEGCIKCPACGFSRC"
     gene            complement(542..1315)
                     /locus_tag="PLM30_05755"
     CDS             complement(542..1315)
                     /locus_tag="PLM30_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOR54711.1"
                     /translation="MKNLVRMKLEAKGTVIGPFVGIDSPDLVEIMGLAGFDFCVIDTE
                     HGPMDPGSIQGLVRAAELSGMTPVVRVPRAEPVDILKVLDVGAHCIHVPQVNTVEAAE
                     DVARAARYHPEGMRGVAVPRALSYGLKPLGASLEEANREVMVIAHCESAAGLEKLDGI
                     AAVEGIDMIFVGPYDLSQSLGVPGEIFHPSMVEAVARALDAINRAGKPAGIFVTTPEE
                     AARRIDEGFRYIGYSVDGLIFGKACSEVVNTLKNRRRGT"
     gene            complement(1475..1750)
                     /locus_tag="PLM30_05760"
     CDS             complement(1475..1750)
                     /locus_tag="PLM30_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR54712.1"
                     /translation="MNKSELVQAVAKSVGLTNTKAAQVVSTVIGEIAGTLAKGDKVQI
                     AGFGTFEVRQRAAREARNPQDPTKKVKVPARKVPAFRAGKALKDKVR"
     gene            complement(1925..2131)
                     /locus_tag="PLM30_05765"
     CDS             complement(1925..2131)
                     /locus_tag="PLM30_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOR54713.1"
                     /translation="MQMQGTVKWFNEAKGYGFITPDEGNDVFVHFSAIQGEGFKTLAE
                     GQRVEFEVVQGEKGPQAANVEKIS"
     gene            complement(2214..>2564)
                     /locus_tag="PLM30_05770"
     CDS             complement(2214..>2564)
                     /locus_tag="PLM30_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR54714.1"
                     /translation="PAAIGPYSQAVWAGDLLFVSGQICMDPKTGALVGETAAEQADQV
                     LRNLGAILESQGLSFANVVKATVYAKDMNDFQAVNEVYARFFDADPPARVFIEVARLP
                     KDVLVEVDAIAVKG"
BASE COUNT          446 a          842 c          777 g          499 t
ORIGIN      
        1 tggaagaccc actgcaaggg catcaccgtc ttccgcgacg ggtgccgttc gggccaggtc
       61 ctctacgtgg acaagcccgc cgtccagcag gctctgccga taccgcaggt gtcggaggtg
      121 gagagcccca tggagcgccc cttcatcctt ggggggaaga ccgccaagat acccaccagt
      181 tacggcaacc tttacctgac ggtcaacgag gtgaacggac ggcccgtgga ggtcttcacc
      241 accatgggca aatcggggca tgagacgatg gccttcaccg aggccatggg cagactgatc
      301 tccctttcgc tgaggagcgg cgtgaggctc cactccataa tcaagcagat gaagggcatc
      361 ggcggttccc agccggtatg gcacggcgac ggcgtcatca tgtccgtgcc cgacgcgata
      421 gcctacgggc tgctcagcat gggttacctg gacatggagg agaagggtat gatggagtcc
      481 ctctccgata cggacatgtg ccccgtctgc ggtgcctcca tgaccaggca ggaggggtgc
      541 atcaagtgcc ccgcctgcgg tttttcaagg tgttgacgac ctcggagcag gctttgccga
      601 agatgaggcc gtccacgctg tagccgatgt agcggaagcc ctcgtcgatg cggcgcgccg
      661 cctcctcggg ggtcgtgacg aagatccccg cgggcttccc ggcgcggttt atcgcgtcga
      721 gggcccgcgc gacggcctcc accatggagg ggtggaatat ctcgcccggc acccccaggg
      781 actgggagag gtcgtagggc cccacgaaga tcatgtctat cccctccacg gcggcgatcc
      841 cgtccagctt ctccaggccg gcggcgctct cgcagtgggc gatcaccatg acctcgcggt
      901 tggcctcctc cagcgacgcc cccagcggct tcagcccgta ggagagggcc ctcggcacgg
      961 ccacaccccg catgccctcg gggtggtagc gggcggcccg cgccacgtcc tcggccgcct
     1021 cgacggtgtt cacctgggga acgtggatgc agtgggcgcc cacgtcgagg accttaagga
     1081 tgtccacggg ctcggcgcgg gggacgcgga ccacgggggt catccccgag agctccgccg
     1141 cccttaccag cccctggatg gaccccgggt ccatggggcc gtgctccgtg tcgatgacgc
     1201 agaagtcaaa gcccgccagg cccatgatct ccaccaggtc ggggctgtct atccccacga
     1261 agggccctat caccgtcccc ttcgcttcga gcttcatcct gacgaggttt ttcatatccc
     1321 atggcctctc tttcttttaa aaaagttccg gtgccccgcg acgtccctct tcagcggcat
     1381 gggggctggc tacgaaggcc gacaggtccg ggcggtcccg gggcaataaa aaaatccgcc
     1441 ccgtcgaggg gcggaagggg aataagacga aaacctagcg gaccttgtcc ttgagagcct
     1501 tcccggcgcg gaaggcggga acctttctcg ctgggacctt gacctttttc gtggggtcct
     1561 gggggttgcg cgcctctctc gccgctctct gcctgacctc aaaagtgcca aaacccgcga
     1621 tctggacctt gtcgcccttg gccagggtcc cggctatctc gccgatcact gtcgatacca
     1681 cctgggccgc cttggtgttg gtgaggccca ccgatttagc cacggcctgg accagttccg
     1741 atttgttcac tgctgtcatc ctcctctcga aaaggctttg ttcaagggga ttatcccatc
     1801 ccttgcgccg cagggcaagc cctgcgaggg tttttcccca tccttcccgc ccttaaaaaa
     1861 gcggcatccc cttggggatg ccgccgctcg tcatggttcg cccgctatgt cttgaggaat
     1921 gaggtcagga gatcttctcc acgtttgcgg cctgggggcc cttttctccc tgcaccacct
     1981 cgaactcgac acgctgtcct tccgccagtg tcttgaagcc ctcaccctgg atcgcgctga
     2041 agtggacgaa gacatcgttg ccttcgtcgg gggtgatgaa accgtacccc ttggcttcat
     2101 tgaaccattt gaccgtaccc tgcatttgga taatgcctcc catacaaaag gatctgccgg
     2161 ccttttaacc ggttgataaa acagtacgta ttatccgccc ctttgtcaat gctttagccc
     2221 ttaacggcga tggcgtccac ctcgaccagg acgtccttcg gcagccgcgc cacttcgatg
     2281 aagaccctgg cgggggggtc ggcgtcgaaa aacctggcgt agacctcgtt gacggcctgg
     2341 aaatcgttca tatccttggc gtaaaccgtg gccttcacca cgttcgcgaa ggagagcccc
     2401 tgggactcga ggatggcgcc caggttcctg aggacttgat cagcctgctc agcggcggtc
     2461 tcccccacca gggcgcccgt cttcgggtcc atgcagatct ggcccgaaac gaaaagcagg
     2521 tcgcccgccc aaacggcctg gctgtaaggg cctatcgcgg ccgg
//
LOCUS       DAOSRG010000176         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_36861_length_2556_cov_9.282687, whole genome shotgun sequence.
ACCESSION   DAOSRG010000176 DAOSRG010000000
VERSION     DAOSRG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36861_length_2556_cov_9.282687"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1135)
                     /locus_tag="PLM30_05775"
     CDS             complement(38..1135)
                     /locus_tag="PLM30_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR54715.1"
                     /translation="MLPEKTVAVVAGKEETDREDVLDVLRCRQSVIEGLLLRGFRAYP
                     VDILKEDLFVKGGVARKLRERSPDCVFNLFEGFGEASYLEIRFAEILESMGIPFTGNP
                     SRALALCLDKDLARRTLEAAGLPVAPGRCVLPGYGRNIAEGLKYPLFVKPRMEDSSVG
                     IDNRSLVASEDGLPRLLDERLALFPDGLIVEEYIDGREYNTGFMGSYPYDLMAVSSIR
                     YAPGTEAGSFLGYESKWDTGSDEYRNSVSVPEEDIAAGTRKEIIRLSREAGRVMGCRG
                     YFRVDLRERRGRLYILEVNPNPDINIDSGFAKQSAKRGLSYPGMVARLVHTAMDRRTP
                     HGRKVPRKYQGRVVVGKKDGGLFGARTGRSR"
     gene            complement(1125..2213)
                     /locus_tag="PLM30_05780"
     CDS             complement(1125..2213)
                     /locus_tag="PLM30_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR54716.1"
                     /translation="MKKEDPGSSFSGAGPVRRIGITFNLKKKELLPGESDDRYEEYDC
                     LETIETLEREIKRLGFETARYEQDETFLERISQNPPDFVLNIAEGRGTTRGRESQVPS
                     VLESLGVPFSGSDSIAIAVTLDKWLTHHVLAGGGAALPDLYMFTAEKDLGRALSIFEK
                     RREYIVKPRWEGSSKGVFPDSVVDNPVDMADRVRKIWKNYNQPALVEEFLPGAEITVG
                     IMGNRNPAVIGMMAIDPKAVKGKTVYSLDHKRDWKEKVRYLGPETIDPRLRNAVGSEA
                     LKVFRILELKDVARVDFRLDVEGVPKAIDINPLPGISEAYSDLPILYRLSGGDFPSLL
                     RGILKEAFQRQGLRWPRTSMKARNRNAA"
BASE COUNT          464 a          827 c          692 g          573 t
ORIGIN      
        1 accctcctcc ggtgttagca ggcaatcctc caggacctca tcgagaacgg ccagttcgcg
       61 ctccgaaaag gcccccgtcc ttcttgccaa cgacaacgcg tccctgatac tttcgcggga
      121 cctttctccc atggggcgtc ctcctgtcca ttgccgtgtg gaccagccgc gcgaccatgc
      181 cgggatagct taatcccctc ttggcgctct gcttggcgaa accgctgtcg atgtttatgt
      241 cggggttcgg gttgacttcc agtatataga gtctgcccct tctctctctc aggtccaccc
      301 tgaagtaacc ccgacaaccc atgacccggc cggcctccct ggagagcctt attatctcct
      361 tccgggtccc ggccgctatg tcctcttctg ggaccgagac ggaattgcgg tactcatcgc
      421 tccccgtatc ccactttgac tcgtaaccga ggaaggaccc cgcctcggtg ccgggcgcgt
      481 aacggatgga ggatacggcc atcaggtcgt aagggtagga tcccatgaac cccgtgttgt
      541 actccctgcc atcgatatac tcctcgacga tcagtccgtc ggggaaaaga gctagcctct
      601 cgtcgagaag cctgggcagg ccatcctctg aggccaccag ggacctgtta tcgatgccga
      661 cactgctgtc ttccatcctg ggtttgacaa agagagggta cttcagtccc tccgcgatat
      721 tacggccata accgggcaga acgcacctcc ccggggccac gggcaaccct gccgcctcaa
      781 gggtcctcct cgccaggtcc ttgtcaagac agagggcaag tgccctggag gggttgccgg
      841 tgaaggggat gcccattgat tcaaggatct cggcaaacct tatttccagg taggaggcct
      901 cgccgaaccc ctcgaagagg ttgaagacgc agtccggtga gcgctccctg agcttcctgg
      961 ctaccccgcc cttgacgaag aggtcttcct tgaggatatc gacggggtat gcccggaacc
     1021 cccgcaagag gagcccctcg ataacgctct gcctgcaacg caggacatcg agaacgtcct
     1081 cgcggtcggt ctcttctttc cctgctacga cggcaacggt cttctcaggc agcatttcgg
     1141 ttcctggcct tcatcgaggt ccggggccac cggagcccct gcctctggaa cgcttccttc
     1201 aggatacccc tcaagaggga ggggaaatcg cccccgctca gcctgtaaag tatgggaagg
     1261 tcactgtagg cttcggagat ccccgggagc gggttgatat ctatcgcctt cggcacgcct
     1321 tcgacgtcga gccggaaatc cacccttgcg acatccttca gttcaaggat gcggaacacc
     1381 ttcaaggcct ccgagcctac ggcgttccgc aggcgcgggt cgatcgtctc cgggcccagg
     1441 taacgcacct tctccttcca gtccctcttg tggtccaggg aatagacggt cttccccttc
     1501 acggctttag ggtctatggc catcatcccg atgacggccg ggttcctgtt gcccattatg
     1561 ccgacggtta tctccgcccc tgggaggaac tcctccacca gggccggctg gttgtaattt
     1621 ttccatatct tgcgcaccct gtcggccata tcgacgggat tatccacgac cgagtcggga
     1681 aagactccct tggaagaacc ttcccacctc ggcttaacga tgtattccct gcgcttttca
     1741 aagatggaca gggcacggcc gaggtccttc tcggcggtga acatgtagag gtccgggagg
     1801 gcagcccccc cgcccgcaag cacatggtgg gtcagccact tgtccagggt gacggctata
     1861 gctattgagt cggacccgga aaaggggacc ccgaggctct ccaggacgct gggaacctgc
     1921 gattcccttc cccttgtggt ccctcggcct tcggctatgt tcaagacgaa gtcggggggg
     1981 ttctgggaga tcctctctag aaaggtttcg tcctgttcgt aacgcgcggt ctcgaaaccg
     2041 agccgcttga tctccctttc gagggtttcg atcgtttcca ggcagtcata ttcctcgtaa
     2101 cggtcgtccg actcacctgg aagcaattcc tttttcttca ggttgaaggt gatgccgatc
     2161 cgccggaccg ggccagcacc gctgaaggat gatccaggat cttccttctt catttcttat
     2221 tcgccccctg cgttaatgga tacaccgacc cttaggccgg tgccgtgact ggtgatatgc
     2281 cccgtcatcc agtccttcga aagacttcac ctccgccttc gagcccggcg tccctacctc
     2341 cacatcctat gccgctttcg cggccctttg gagtcccgcc gacgtagcgg gctcacgtag
     2401 gtcacccgtc acaccagtgg tcctttccag aagatcagcg cccgggggcc ttaaggggct
     2461 tattggcgcc gaccctgatg cgaagttgaa ggccagactc tcgatatgcc tctttcttcg
     2521 cggattatac atccctcttt ctagattaca ataggt
//
LOCUS       DAOSRG010000177         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_36941_length_2551_cov_11.990385, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000177 DAOSRG010000000
VERSION     DAOSRG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36941_length_2551_cov_11.990385"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="dctP"
                     /locus_tag="PLM30_05785"
     CDS             <1..860
                     /gene="dctP"
                     /locus_tag="PLM30_05785"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOR54717.1"
                     /translation="KISLHYAGELVDYAEVDKALQDNMIQISNTSTLFFRGSIPMGWV
                     SAPNMPPFITRTNDEFNELFHHRGVDRLVNEALEERGIKYFGSHSVGNTYFWSKKPIR
                     TLDDLKGFKVRFFGSMSDFVEHFGAAPVMLPHPETYMAIATGTLDGSGTAWWLYRDLK
                     LYEVCPYFIGPAMQVPQGMELWASKKAWDALPEDIQAMVETAAHVFNQDYMDAVTMEE
                     REMFNKSFPEWGTTYIEFSDADVKRIIDEFSLPYLDKVAAEQGPKDARVAEAVEIIKQ
                     FMRDYGYID"
     gene            958..1503
                     /locus_tag="PLM30_05790"
     CDS             958..1503
                     /locus_tag="PLM30_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOR54718.1"
                     /translation="MNILTGFITTVETVNDRMGKFVAFLVYPTMLVLVYEVVMRYAFG
                     RPTIWAHETSCMLYGAHFILGGAYALRHNAFVNVEVFYMKFPKRVKAIVDLFTWTMFY
                     AFVGVMLWKSLPWAWESLRVNEFSDSTWGPLLWPIKWTIPIAAVFMLLQGMTKTIKDL
                     HLALTGREIIAAADAEATGGN"
     gene            1513..>2551
                     /locus_tag="PLM30_05795"
     CDS             1513..>2551
                     /locus_tag="PLM30_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR54719.1"
                     /translation="MGIEMISILLFGGMFLLLALGLPVAFVLGGLATVFTAVFWGPES
                     LFIIVARTFSMMSTTTLVAAPLFVLMATVLERSGVAEGLYEMMYRWSGGLRGGLAVGT
                     VLACTLIAAMSGIASTGVVVMGVMALPAMLKRGYDKRLATGCILAGGVLGPLIPPSIS
                     LVLYGTIAQVSIGGLFAGGMGAGILCSFLIIGYILLRCYINPELGPAIPLEERADWKG
                     KVASLKGVILPLLLIIAVLGSIYSGIATPTEAAAVGALGAFVCSAIHRRLNWELIKSV
                     AYTTIQVQGFMMWILFAAQGFAAVYMGLGASRMVIKLVETYEIGWWTMLIGIQVVWFL
                     LGCVIDAWSILM"
BASE COUNT          470 a          763 c          776 g          542 t
ORIGIN      
        1 tgaagataag cctgcactac gccggggaac tggtcgatta cgccgaggtg gacaaggccc
       61 tgcaggataa catgatccag atctccaaca ccagcaccct gttcttccgg gggtccatcc
      121 ccatgggctg ggtctcggcg cccaacatgc cgcccttcat caccaggacc aacgacgagt
      181 tcaacgaact cttccatcac cggggggtcg accggctggt gaatgaggcc cttgaggaga
      241 ggggcatcaa gtacttcggc agccatagcg ttggcaacac ctacttctgg agcaaaaaac
      301 ccatccggac cctggacgac ctgaaggggt tcaaagtgag gttcttcggc tccatgtcgg
      361 acttcgtgga gcacttcggg gccgccccgg tgatgctccc gcaccccgag acctacatgg
      421 cgatagccac ggggaccctc gacggcagtg gtacggcgtg gtggctctac cgtgacctca
      481 aactctacga agtgtgcccc tacttcatag gacctgccat gcaggtgccc caggggatgg
      541 agctctgggc ctcgaaaaag gcctgggacg cccttcctga ggacatccag gccatggtcg
      601 agacggccgc ccatgttttc aaccaggact acatggatgc cgtcaccatg gaggagcggg
      661 agatgttcaa caagtccttc cccgagtggg gcacgaccta catcgaattc agcgatgcgg
      721 acgtgaagag gataatcgat gaattctcac tgccttacct ggacaaggtg gcggcggaac
      781 agggcccgaa ggatgccagg gttgccgaag ccgtcgagat aatcaagcag ttcatgaggg
      841 attacggtta catcgactga cgctggagac cctgcttaag cacccccttc tccaccaggg
      901 aggggggttc ttttttacag tccctcggaa accgaggctt acgatggggt gaagcgattg
      961 aacattttga ccggtttcat cacgaccgtt gagacggtta acgaccggat gggcaagttc
     1021 gtggcctttc tcgtctaccc gacgatgctc gtcctggttt acgaggtggt aatgaggtat
     1081 gccttcggca ggcccaccat ctgggcccac gagacttcct gcatgcttta cggggcccac
     1141 ttcatcctgg ggggggccta cgccctgcgg cataacgcct tcgtcaacgt ggaggtcttc
     1201 tacatgaaat ttcccaagcg tgtgaaggcc atcgtcgacc ttttcacctg gaccatgttc
     1261 tacgccttcg tgggggtcat gctctggaag agcctcccct gggcctggga gagcctgagg
     1321 gtgaacgagt tcagcgacag cacctggggc cctctccttt ggccgatcaa gtggaccata
     1381 cccatcgccg ccgtcttcat gctgctccag gggatgacga agacgatcaa ggacctgcac
     1441 ctcgccctta cggggcggga gatcattgcc gcggccgacg cggaagccac gggcgggaat
     1501 tgaggtggac ccatgggtat agagatgatc tccatccttc ttttcggcgg catgttcctc
     1561 ctgttggccc tgggacttcc cgtagctttc gtcctgggcg gcctggccac ggtcttcacc
     1621 gccgtattct ggggccccga gtcgctcttt atcatcgtgg ccaggacctt ttccatgatg
     1681 tccaccacga cgttggtggc ggctcctctc ttcgtgttga tggcgactgt ccttgagcgc
     1741 tcgggcgtgg cggagggcct ttacgagatg atgtaccgct ggtccggagg gctaaggggt
     1801 ggtttggccg tgggtaccgt tctggcctgc acgctgatag ccgccatgtc ggggatagcc
     1861 tcgacgggag tcgtcgtcat gggtgtcatg gccctcccgg ccatgcttaa acgggggtac
     1921 gacaagagac tggccacggg ctgcatcctg gccggcggcg tcctggggcc tctcatccct
     1981 cccagcatct ccctggtcct ttacggcacc atcgcccagg tctccatcgg cggcctcttc
     2041 gccggcggta tgggagccgg gatcctctgt tccttcctga taatcggcta catattgtta
     2101 aggtgctata tcaacccgga actcggcccg gccatccccc tcgaggagag ggccgactgg
     2161 aagggcaagg tcgcttccct caagggtgtg atccttcccc tgctcttgat catagccgtc
     2221 ctgggttcca tctactcggg gatcgccacg ccgaccgagg ccgccgccgt gggggccttg
     2281 ggcgccttcg tatgcagcgc catccacagg cggctgaact gggaactgat aaaaagcgtg
     2341 gcttacacca ccatccaggt tcagggtttc atgatgtgga tactcttcgc cgcccagggc
     2401 ttcgccgccg tctacatggg cctgggcgct tccaggatgg tcatcaagct ggtggagacc
     2461 tacgagatcg ggtggtggac gatgctgata gggatacagg tcgtctggtt cctcctgggc
     2521 tgcgtcatcg acgcctggag catcctgatg a
//
LOCUS       DAOSRG010000178         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_37620_length_2517_cov_8.538993, whole genome shotgun sequence.
ACCESSION   DAOSRG010000178 DAOSRG010000000
VERSION     DAOSRG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37620_length_2517_cov_8.538993"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="deoC"
                     /locus_tag="PLM30_05800"
     CDS             complement(<1..723)
                     /gene="deoC"
                     /locus_tag="PLM30_05800"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOR54720.1"
                     /translation="MIAVTDIELLSKRARIKAAKAEAGLFERPSPERVRGSIDHTLLA
                     AGASVEDVTALCDEAVRFGFASVCVPLSCVPDARRFLSGSAVKVSSVAAFPLGATPIP
                     VKTAEVRWALENGADEVDAVVDIRAARALDRGALRDDLSALREASRGAVLKVILEMPL
                     LNDVQAVTAVMLAEQAGVDFVKTCTGFAGGVTFYHVALLRTAASDRVRIKASGGIRTY
                     AAAEILVALGADRLGTSKATQFL"
     gene            complement(720..1079)
                     /locus_tag="PLM30_05805"
     CDS             complement(720..1079)
                     /locus_tag="PLM30_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54721.1"
                     /translation="MTDPKGDHERVFSENYARAAARVLEAVSRTAQLSSLATPEVQDL
                     FENWLEILGRQILRDQDVPGRMDIPSRAAEIGVSASSLLGLLLYLQRQGRVSITEVLL
                     APGCGRDEDICDCIRQG"
     gene            1217..2278
                     /locus_tag="PLM30_05810"
     CDS             1217..2278
                     /locus_tag="PLM30_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOR54722.1"
                     /translation="MTTALRILLLGFGNVGRAFCEIVEEKAPLLRDLFGVEGRFVGIA
                     TRSRGCVSDPGGIDIGALLKKESSTGRLGPAPSRGGRDAPSAVEMVQEMDYDLLVVCT
                     STTIQSPEEPKSFICGGLQRGKDVITSNKGPLFLGYREIRDFAAANGALFLFEAAVMG
                     GTPFFSFARHCLTGSEVLSCEGVMNTTSNYVLDLMVAGASFDDALLDARRKGYAEADP
                     TFDLEGWDSAVKASIIAQVLMGAPPVSLEDIEVQGINQIKTRLLLEAAHSGGALRLVS
                     RVEKGKDGVAITVRPEVLSPSNPLSRVHGATNGVLFLTDTLGEIFVAGGGIGPRHTAF
                     SLLRDLIEVACSRKGGPEC"
     gene            2272..>2517
                     /locus_tag="PLM30_05815"
     CDS             2272..>2517
                     /locus_tag="PLM30_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR54723.1"
                     /translation="MLIVDLHTHSTFSDGTFSPEALVGRAAKRHVSILSLTDHDTTAG
                     LDRFLAACGKKGIQGITGVELSAEAPFTLHIMGYRIDS"
BASE COUNT          434 a          854 c          778 g          451 t
ORIGIN      
        1 caaaaactgc gtcgccttcg atgtccccag cctgtcggcc cccagggcca cgaggatctc
       61 cgccgccgcg taggtcctga tcccgccgga agcctttatc cttactctgt cggaggccgc
      121 cgtccttaaa agggccacgt ggtaaaaggt gacgcccccc gcaaagcccg tgcaggtttt
      181 tacgaaatcc acgccggcct gttcggccag catcaccgcc gtgaccgcct ggacgtcgtt
      241 cagcaggggc atctcgagga tcaccttgag gacggccccc cgggaggctt ctctcagcgc
      301 tgaaaggtcg tcccggaggg cccccctgtc gagggccctg gcggccctga tatccaccac
      361 ggcatccacc tcgtcggccc cgttctccag ggcccagcgg acctcggcgg tcttcaccgg
      421 tatcggggtg gcccccaggg ggaaggccgc aacggaactc accttgaccg cggaccccga
      481 gaggaaccgc cgggcatcgg gcacgcagct caaggggacg cagaccgagg cgaacccgaa
      541 gcggaccgcc tcgtcgcaca gggctgtcac gtcttccacg gaggcaccgg cggcgaggag
      601 ggtatggtct atggagcccc ggaccctctc cggcgagggg cgttcgaaga gcccggcctc
      661 ggccttggca gcctttatcc tggcccgctt ggagagcagt tcaatgtcgg tcacggctat
      721 cacccctgcc ttatgcagtc gcagatatcc tcgtcgcggc cgcaccccgg cgcgaggagg
      781 acctcggtta tactgaccct gccctgcctc tgtagataaa ggagcaaccc cagcagggag
      841 gaggcggata cgcctatctc ggccgccctc gaggggatgt ccatccggcc gggcacatcc
      901 tgatcccgca ggatctggcg gcccagtatc tcgagccagt tctcgaagag gtcctggact
      961 tccggcgtag ccagcgatga aagctgggcc gtcctcgaga cggcctccag cacacgggcc
     1021 gccgccctgg cgtagttctc gctgaagacc ctttcgtgat caccctttgg atcggtcatt
     1081 acccgtggcc tcccgttctg ttcttctctt ccaggggaag atgccttttt cggatcgctc
     1141 ccgggcattc ctctctggaa gaccagacgc aatgatatca tagaccccaa tggctttgat
     1201 gaccgtccgt cgggaggtga cgacagcctt gaggatcctt cttcttggtt tcggcaacgt
     1261 ggggagggcc ttctgcgaga tcgtcgagga gaaggcccct cttctgaggg atctgttcgg
     1321 cgtcgagggc cgttttgtgg gtatagcgac ccgttcgaga ggttgcgtga gcgaccccgg
     1381 agggatagac atcggtgccc tcctgaaaaa ggaatcctcc acgggcaggc tcggcccggc
     1441 tccctcccgg ggggggcggg acgctccctc ggcggtggag atggtccagg agatggatta
     1501 cgacctcctc gtcgtctgta cctccaccac catccagtcc ccggaagagc caaaaagctt
     1561 catctgcggg gggcttcagc ggggcaagga tgtgatcacc tccaacaagg gtcctctctt
     1621 cctggggtac cgggagatac gtgacttcgc cgctgccaat ggggccctct tcctgttcga
     1681 ggcggccgtc atgggcggca cccccttctt ctccttcgcg aggcactgcc tgaccgggtc
     1741 ggaagtcctc tcctgcgaag gcgtcatgaa caccacctcc aattacgtcc tcgacctcat
     1801 ggtcgccggg gcctccttcg acgacgccct cctcgacgca aggaggaagg gttacgccga
     1861 ggcggacccc accttcgacc tcgaaggttg ggacagcgcc gtcaaagcct cgatcatcgc
     1921 ccaggtcctc atgggcgcgc cccccgtctc cctggaagac atcgaggtcc aggggataaa
     1981 ccagatcaag accaggctgc tcctggaggc ggcccattcg ggcggagccc tccgcctcgt
     2041 ctccagggtg gagaagggca aggacggggt cgccatcacg gtcaggcccg aggtcctttc
     2101 gccctccaac cccctctcgc gggtccatgg ggccaccaac ggggtgctct tcctgacgga
     2161 cacgctgggg gagatcttcg tcgccggcgg cggcataggc ccgaggcaca cggccttttc
     2221 cctgctgagg gacctcatcg aggtcgcctg ctcgagaaag gggggccccg aatgctgatc
     2281 gtggaccttc acacccacag caccttcagt gacggcacct tttccccgga agccctggta
     2341 gggagggccg ctaaaaggca cgtgtcgatc ctgagcctca ccgaccacga cacgaccgcc
     2401 ggcctcgaca ggttcctggc ggcctgcggc aagaagggga tccagggcat taccggggtg
     2461 gaactgtccg ccgaggcccc cttcaccctt cacatcatgg gttaccgtat cgacagc
//
LOCUS       DAOSRG010000179         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_37784_length_2510_cov_9.200000, whole genome shotgun sequence.
ACCESSION   DAOSRG010000179 DAOSRG010000000
VERSION     DAOSRG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37784_length_2510_cov_9.200000"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLM30_05820"
     CDS             complement(<1..1149)
                     /locus_tag="PLM30_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOR54724.1"
                     /translation="MADVKELWKEVESGIEHFSKRAIDLSDWLAKNPELSEKEFEASK
                     KHAAFLEEAGYDVQAPFMGLATSFFSTKGDPAGPKVCLLFEYDALPGVGHGCGHNLSG
                     AMSGLAAAGLAGVMDKTGGQLVLVGTPAEETNGSKVVLAEKGVFDSLDLAMMVHSSDV
                     STYAGYRSLAMDAVEFRFTGKPAHAASEPWAGRNALNGVQLFFHAVDMLRQHIIPEAR
                     VHGIIPEGGLAPNIVPEHAAARFYFRAPKRPILDSIVSRIFNCALGAAQATETEVTWR
                     NYEYSFDDMLKNDTAEEFVEGILAEFGVPFIPTPGPQGSSDVGNVSYRCPAIQPKLSI
                     VDEPMTLHTHEFAEATITERGHRALVTGARLMARTALAVFLDEDLRRRI"
     gene            complement(1164..>2510)
                     /locus_tag="PLM30_05825"
     CDS             complement(1164..>2510)
                     /locus_tag="PLM30_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR54725.1"
                     /translation="RCTDMDIVVRNGTVVDPVSLEEKRVDVAIKDGVFAFVGSGAPEG
                     VRELDASGLLVLPGLIDTHMHFEHRVGDPYTVLKSLLLQGVTTAIGGHCGEGVMMKVY
                     KDWFSQNPVLNMGFMIGATTLRKAVGADDRYSPATSEQVGMMEPLLEENLHEGALGLS
                     FGLEYAPGTSRDELFRLAQVVSRFKHRFISIHVRYDGRRSPEAVAEAIEISKRSGVRV
                     QVSHLGSMTAFGHSRDAIGMIEQARREGVDVTFDVYPYYAFAARIGSAVFDPGFEQRL
                     GKGLESLEVSTGRFKGVPLTAELFARAREEDPDAYVIAHVMNPEEVDLCLLHKDSAIA
                     SDAVLRGDEGHPRAAGTFPRGIGILRKAGLSWPEAVRHATSRPAEMIWHRGGRITEGA
                     NAELAVIDPDSYEDRGRFGAPLTAPGGVKWVVLNGVVVVEGCEIVGPPSGRILLGE"
BASE COUNT          435 a          819 c          784 g          472 t
ORIGIN      
        1 gatcctccgg cgcaggtctt catcgaggaa caccgccagg gccgtcctgg ccatcagcct
       61 ggcccccgtc accagggccc ggtggcccct ttcggtgatg gtcgcctcgg cgaattcatg
      121 ggtatgaagg gtcatcggct cgtcgacaat gctcagcttg ggctgaatcg ccgggcagcg
      181 gtaggagacg ttgcccacat cggaagatcc ctggggcccc ggggtgggga taaagggcac
      241 cccgaactcc gccaggatgc cctccacgaa ttcctccgcg gtgtcattct tgagcatgtc
      301 gtcgaagctg tattcatagt ttctccaggt cacttccgtc tccgtggcct gggccgcgcc
      361 gagggcgcag ttgaatatcc tggagacgat gctgtcgagg atgggcctct tcggggcccg
      421 gaagtagaac ctcgcggcgg cgtgctccgg cacgatgttc ggtgcgagcc ccccctcggg
      481 gatgatgccg tggacccggg cctcggggat gatgtgctgg cggagcatgt ccacggcgtg
      541 gaagaagagc tgcaccccgt taagggcgtt cctccccgcc cagggttcgc tcgccgcgtg
      601 ggcgggcttg ccggtgaaac ggaactcgac ggcgtccatg gcgaggctgc ggtaaccggc
      661 gtaggtggag acgtcgctgc tgtggaccat catggccagg tcgagggaat cgaacacgcc
      721 cttttcggcc aggacgacct tggacccgtt ggtctcttcg gcgggggtcc ccaccaggac
      781 cagctgaccg ccggtcttat ccatgacgcc ggcaagaccg gcggccgcga ggccggacat
      841 ggcccctgaa aggttgtggc cgcagccgtg gccgacgccg ggcagggcgt cgtactcgaa
      901 caggaggcag actttcggac ccgcggggtc tcccttcgtt gaaaaaaagg aggtggccag
      961 tcccatgaag ggcgcctgga catcgtagcc ggcctcttca aggaaggccg cgtgcttttt
     1021 actggcttcg aactcttttt cggacaactc cgggttcttc gctagccagt cgctgagatc
     1081 gatcgcccgc ttcgagaaat gctcgatccc ggactcgact tccttccata gctctttcac
     1141 atcggccatt acggggcacc cccttattca cccagaagga tcctgccgct gggagggccg
     1201 acgatctcgc acccctccac gacgaccaca ccgttgagaa cgacccattt tacccccccc
     1261 ggagcggtca ggggcgctcc gaagcggccc cggtcctcat aggagtccgg gtcgatcact
     1321 gccaattcgg cattggcacc ctcggtgatg cgtccaccac ggtgccatat catctccgca
     1381 ggcctggacg tggcgtgcct caccgcttcg ggccaggaaa gacccgcctt cctgagtatc
     1441 cctatccccc ttgggaaggt gcccgcggcc ctggggtgcc cctcgtcacc ccggagcacg
     1501 gcgtcggagg ctatagcgct gtccttatga aggaggcaca ggtccacctc ttcggggttc
     1561 atcacgtggg cgatgacgta ggcgtcgggg tcctcttccc tggccctggc gaagagttca
     1621 gccgtcaggg ggacaccctt gaaccgcccg gtggatacct ccaggctctc aaggcccttc
     1681 cccaggcgct gttcaaagcc cgggtcgaag acggccgaac ctatcctggc ggcgaaggcg
     1741 taatagggat aaacgtcgaa ggtgacatcc accccctcgc gccgggcctg ctcgatcatc
     1801 ccgatagcat cgcgggaatg gccaaaggcc gtcatgctcc ccaggtggga cacctggacc
     1861 ctgacgccgc tccgcttcga gatctcgatg gcctccgcta cagcctcggg actcctcctg
     1921 ccatcgtacc ttacgtggat ggatatgaac cggtgcttga accgggaaac gacctgggcg
     1981 aggcggaaca gttcgtcccg ggaggtacca ggggcgtact cgagcccgaa ggacaggccc
     2041 agggccccct catggaggtt ttcctcgaga aggggttcca tcataccgac ctgctcggag
     2101 gtggcgggcg aatacctgtc atcggccccg acggcttttc tcagggtcgt ggccccgatc
     2161 atgaacccca tgttcaggac ggggttttgg ctgaaccagt ccttgtaaac cttcatcatg
     2221 acgccctcgc cacagtgtcc gccgatggcg gtggtgacgc cctgcagcag cagcgacttg
     2281 agaacggtat aggggtctcc tacccggtgc tcgaagtgca tgtgggtgtc gatcagcccc
     2341 gggaggacca ggagccccga ggcgtcgagt tcacggaccc cttccggagc gcctgagccc
     2401 acgaaggcga atactccatc ttttattgcc acatcgaccc ttttctcctc aagggagacc
     2461 gggtcgacga cggtcccgtt cctcacaacg atatccatgt ccgtgcacct
//
LOCUS       DAOSRG010000180         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_38134_length_2493_cov_8.751025, whole genome shotgun sequence.
ACCESSION   DAOSRG010000180 DAOSRG010000000
VERSION     DAOSRG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38134_length_2493_cov_8.751025"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..567
                     /locus_tag="PLM30_05830"
     CDS             31..567
                     /locus_tag="PLM30_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR54726.1"
                     /translation="MLLVVAAVLVLYVWRTYNTMVRSRNLVDEGWSGIDVQLKRRADL
                     VGNLVETVKGYAKHEKELFEKVTQARAAAVSAGSVGERAQAENALTGTIRSLFAVAEN
                     YPELKANTNFLDLQKNLGELEAEIQMARRYYNGTVRAFNSLIQVFPNNMLAGPFGFTS
                     REYFEAAEEDRAVPRVQF"
     gene            596..>2493
                     /locus_tag="PLM30_05835"
     CDS             596..>2493
                     /locus_tag="PLM30_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOR54727.1"
                     /translation="MRTFRRALAAVLLLLALVPAASAAERILSFDSSIGVGRDGTLTV
                     REDITVLVEHVQIQRGLLRDFPTDYTSPGGGRVRVGFTVQTVLLDGGSVPWKTERISN
                     GVRVRIGDPGSYAPLGEHTYTIVYTTTEQVGFFEDHDELYWNVTGTGWDFPIDRASAL
                     VTLPEDVPVEKIAWYTGPQGSRAQDAMGRTAGNTAWFQTTVPLGVREGLTIVAGFPKG
                     FIHPSKDYLKRQARAAFAERWGKYLPPLAVVLVLIYYILVWSRWGKDLRPGTIIPLFY
                     PPEGITPAMASYVYNQKLRDDTFTATLIDLGVRSYLRIEEHDVPKGLFSRGKNRFTLH
                     PTDKDRNGLPLVENAFLASLFPGGRSVDVDKENHEVLSAAKGAITDHVAYRGKDYFLR
                     NWKWVGLGVLITLVLMGYITAFLGTWGAGKEDTIFSSIWLGTWTLGIGVMVFSALASL
                     GEGFRRKKAGFFIRGLFLLFFSIPFMGVEALFFRLFAVGLSLNYTLSLAAAFFLNVLF
                     FKLMKNYTPEGRKLMDRLEGLRMYMSAAEKGRIKALARVDMPEDTPRHFESLLPYAIA
                     LGVEKEWAAHFDEILKKAQYDPQWYTGTYPVYTLGAGSFMTGLSGGLGTAVASSSVAP
                     GSGSGFSGG"
BASE COUNT          430 a          859 c          768 g          436 t
ORIGIN      
        1 ctgggggtga tcctcgtgac cgtcctatgg atccttttag tagtagcggc tgtcctggtc
       61 ctgtacgtct ggaggaccta caacaccatg gtccgttccc gcaacctcgt cgacgagggc
      121 tggagcggca tcgacgtcca gctcaagcgg cgggccgacc tggtgggcaa cctcgtggag
      181 accgtcaagg gctatgccaa gcatgaaaag gaactcttcg agaaggtgac ccaagccagg
      241 gcggcggcag tgagcgccgg ctccgtcggg gaaagggccc aggccgagaa cgccctcacc
      301 gggaccatcc gctccctctt cgccgtggcc gagaactacc cggaactgaa ggccaacacc
      361 aacttcctgg acctccagaa gaacctgggc gaactcgagg ccgagatcca gatggcccgc
      421 cggtactaca acggcaccgt gagggccttc aactccctca tccaggtctt ccccaacaac
      481 atgctggccg gccccttcgg cttcacgagc cgcgaatact tcgaggcggc cgaggaagac
      541 cgcgccgtcc cgagggttca gttctagccc ggttttggtc gggagggatc ccttcatgag
      601 gaccttcagg cgcgccctgg cggctgtcct gctgctcctt gccctcgtcc cggcggcatc
      661 cgccgccgag aggatactgt ccttcgacag ctccatcggc gtggggcgcg acggcaccct
      721 caccgtcagg gaggacatca ccgtcctcgt ggagcacgtc cagatccaga ggggccttct
      781 ccgggacttc cccaccgact acacctcacc cggcgggggc cgggtccggg tcggcttcac
      841 ggtgcagacg gttctgctcg acggcgggtc cgtcccgtgg aagaccgaaa ggatcagcaa
      901 cggcgtgcgg gtccgcatcg gcgaccccgg atcctacgcc cccctggggg agcacaccta
      961 caccatcgtc tacaccacca ccgagcaggt cggcttcttc gaggaccacg acgaactcta
     1021 ctggaacgtc accggcaccg gctgggattt ccccatcgac agggcctccg ccctggtcac
     1081 cctgccggag gacgtccccg tggagaagat cgcctggtac accggccccc agggttcccg
     1141 cgcccaggac gccatgggca ggacggccgg taacacggcc tggttccaga ccacggtccc
     1201 cctcggggtc agggagggcc tcaccatcgt ggcgggcttc cccaagggct tcatccaccc
     1261 ttcgaaggac tacctcaaga ggcaggcccg ggcggccttc gccgagcgct ggggcaagta
     1321 cctcccgccc ctggcggtgg tgctggtcct catctactac atcctggtat ggagccgctg
     1381 gggcaaggac ctccgccccg ggaccatcat ccccctcttc tacccccccg agggcatcac
     1441 cccggccatg gcctcctacg tctacaacca gaagctccgg gacgatacct tcactgccac
     1501 cctgattgac ctgggcgtga ggagctactt gaggatcgag gagcacgacg tccccaaggg
     1561 cctcttcagc cgggggaaga accgcttcac ccttcatcct accgacaagg accgcaacgg
     1621 cctccccctg gtggagaacg ccttcctggc ctccctcttc cccggtggga ggtccgtcga
     1681 cgtggacaag gagaaccacg aggtcctctc cgcggcgaag ggcgccatca ccgaccacgt
     1741 ggcctaccgc ggtaaggact acttcctgag gaactggaag tgggtcggcc tcggcgtatt
     1801 gatcaccctc gtcctgatgg gttacatcac ggccttcctc gggacctggg gggcggggaa
     1861 ggaggacact atcttttcct cgatctggct cggcacctgg accctgggga taggcgtcat
     1921 ggtcttttca gcccttgcct ccctgggcga gggcttccgg aggaaaaagg cgggcttctt
     1981 catcaggggc ctcttcctgc tgttcttttc catacccttc atgggtgtcg aggcgctttt
     2041 cttcaggctc ttcgcggtgg ggctgtctct caactacacc ctctccctgg cggcggcctt
     2101 cttcctgaac gtcctcttct tcaaactcat gaaaaactac acccccgagg ggaggaagct
     2161 catggaccgg ctggaggggc tccgcatgta catgtccgcc gccgagaagg ggcgcatcaa
     2221 ggccctggcc agggtcgaca tgccggaaga cacgccccgt cacttcgaaa gcctgctgcc
     2281 ctacgccata gccctggggg tggagaagga gtgggccgcc cacttcgacg agatcctcaa
     2341 gaaagcgcag tacgacccgc agtggtacac gggcacgtac cccgtctata ccctgggggc
     2401 gggttccttc atgacggggc tcagcggggg gctcgggacg gccgtggcgt cctcgtcggt
     2461 ggccccgggg tccggttcgg gcttcagcgg cgg
//
LOCUS       DAOSRG010000181         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_38758_length_2465_cov_15.008714, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000181 DAOSRG010000000
VERSION     DAOSRG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38758_length_2465_cov_15.008714"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="citF"
                     /locus_tag="PLM30_05840"
     CDS             complement(<1..1296)
                     /gene="citF"
                     /locus_tag="PLM30_05840"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOR54728.1"
                     /translation="MTINAAGRNIPDTIPGYGEVRHFKGHDRRPPEGCRFGKRNRAAF
                     NTRRDKTLPDLKAAISASGLKSGMTVSFHHHLRDGDYVVNAVIDACAEMGIRDLVIFP
                     TALFKVHKHLTKHIQNGVIRQIMGSVNGPIGQLASEGGFSVPVVLRSHGGRPRAVTSG
                     DVVIDVAFIAAPTADNYGNLTGAVGRSACGSLGYAHSDAHYAQCVVAVTDNLVNYPAP
                     CIQIPQFFVDFVVPVDKIGDPAGIVSGTTRVTRDPMRLLIARNAAKLIEATPYFKDGI
                     SFQTGAGGISLAVAAYVREAMRERGIKGSFGLGGITEFFVDMMKEGLFERLLDVQSFD
                     LAAVKSIAENPNHVEISADWYANPWNAGCAVNMLDTVVLGATEVDRDFNVNVNTEADG
                     AMLHGIGGHQDTAAGAQLTIITTPLLRGRIPCAVDSVYTV"
     gene            complement(1293..2168)
                     /locus_tag="PLM30_05845"
     CDS             complement(1293..2168)
                     /locus_tag="PLM30_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOR54729.1"
                     /translation="MRRTKLYLPGNNPNMLLRGHLFGPDGVILDLEDAVAMSEKDAAR
                     ILVRDVLKRGDFGDCEVTVRINGMDTEYWKTDIETVVPCGIDGIRIPKVEDPKDIALM
                     DEALSQIESKARIPVGRTLIFCLLETALGVWRAFDIARASKRVAAICPGGEDLRADLK
                     TTRSENSEELIGPRRMIALAAHAAGVDALDTVYADITDDAGLRRETEWVKQLGFQGKS
                     VIHPNQIPVVHDVFTPTAKEIEQAKKIVEAAAEAAARGLGAVQVDGRMVDKPVVKRAE
                     YTLQRAGLKAGGEKA"
     gene            complement(2171..>2465)
                     /gene="citD"
                     /locus_tag="PLM30_05850"
     CDS             complement(2171..>2465)
                     /gene="citD"
                     /locus_tag="PLM30_05850"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF009726.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOR54730.1"
                     /translation="PVPGGKNMKTAQAGTLESMDCIVTVTETEKGRGISIAMEGASIV
                     RFGEAMLKATKDTLKGLGVRDISVSIQDNGAGDLVLKARVEAAVRRFAGVRTP"
BASE COUNT          367 a          816 c          783 g          499 t
ORIGIN      
        1 gacggtgtag accgagtcga cggcgcaggg tatccgcccg cggagcagcg gggtggtgat
       61 gatggtcagc tgcgccccgg cggcggtgtc ctggtgcccc ccgataccgt ggagcatcgc
      121 cccgtcggcc tcggtgttga cgttcacgtt gaaatccctg tccacctcgg tagcgccgag
      181 gaccaccgtg tcaagcatgt tgacggcgca ccccgcgttc caggggttgg cgtaccagtc
      241 ggccgagatc tccacgtggt tggggttctc agcaatggac ttcaccgctg cgaggtcgaa
      301 ggactggacg tccagcagcc tctcgaagag cccctccttc atcatgtcca cgaagaactc
      361 ggtgatgccc ccgagcccga aactgccctt aatcccccgc tcccgcatgg cttccctgac
      421 gtaggccgcc acggcgaggg agatgccccc cgccccggtc tggaaggaaa tgccgtcctt
      481 gaaatagggg gttgcctcga tcagtttcgc ggcgttacgg gctatcagaa gcctcatggg
      541 gtccctggtc accctggtgg tgccgctgac gatgccggcg gggtccccga tcttgtcaac
      601 ggggaccacg aagtcgacga agaactgggg gatctggatg cagggcgccg ggtagttgac
      661 caggttgtcc gtgacggcca ccacgcactg ggcgtagtgg gcgtcgctgt gggcgtaccc
      721 cagggacccg caggccgacc tgccgacggc ccccgtcagg ttgccgtaat tgtccgccgt
      781 gggggcggcg atgaaggcca cgtcgatgac cacgtcgccg ctggtcacgg ccctggggcg
      841 tccgccgtgg ctcctcagga cgacggggac gctgaacccg ccctccgagg cgagctgccc
      901 gatgggtccg ttgacggagc ccatgatctg gcggatgacc ccgttctgga tatgtttcgt
      961 caagtgcttg tggaccttga agagggccgt ggggaagatc accaggtcac ggatgcccat
     1021 ctcggcgcag gcatcgatga cggcgttgac cacgtagtct ccgtcgcgga ggtggtggtg
     1081 gaaggacacg gtcatcccgc tcttgagccc cgaggcgctg atagccgcct tgaggtcggg
     1141 cagggtcttg tccctcctgg tgttgaaggc ggccctgttc ctcttgccga accggcaccc
     1201 ctcggggggt ctcctgtcgt ggcccttgaa gtgccggacc tccccgtaac cgggtatcgt
     1261 atcgggaata ttgcgtcccg cggcgtttat agtcatgctt tctcacctcc ggctttcaga
     1321 ccagcccgct ggagggtgta ctccgccctc ttgaccacgg gcttgtcgac catcctgccg
     1381 tcgacctgga ccgccccaag ccccctggcg gcggcttcag cggcggcctc gacgatcttt
     1441 ttggcctgtt cgatctcctt ggccgtcggc gtgaagacat cgtggaccac aggtatctgg
     1501 ttcgggtgta tgacgctttt gccctggaag cccagctgct tgacccactc cgtttcccgc
     1561 ctcaaccccg cgtcgtcggt tatgtcggcg tagaccgtgt ccagggcgtc gacgccggcg
     1621 gcgtgggcgg ccaaggctat catcctcctg gggccaatga gctcctcgct gttctcggaa
     1681 cgggtcgtct tcaggtccgc ccggaggtcc tccccaccgg ggcagatggc ggccaccctt
     1741 ttcgaggccc tggctatgtc gaaggccctc cagacgccca gggccgtctc gagcaggcag
     1801 aagatcaggg tcctgcccac cggtatcctg gccttgctct cgatctggga tagggcctcg
     1861 tccatgaggg cgatgtcctt ggggtcctcc accttgggta tgcggatgcc gtcgataccg
     1921 cagggcacga cggtctcgat gtcggtcttc cagtactccg tgtccatgcc gttgatcctc
     1981 acagtgacct cgcaatcgcc gaagtcgccc cttttcagga catccctgac gaggatcctg
     2041 gccgcgtcct tttcgctcat cgccacggcg tcctccaggt ccaggatgac gccgtcgggg
     2101 ccgaagaggt gtccccggag gagcatgttc gggttgttcc cggggagata cagcttggtc
     2161 cttctcagcc tcatggggtc ctcacccccg caaaccgtct cacggccgcc tcgaccctgg
     2221 ccttcagcac caggtcgccg gccccgttgt cctggatgga gacggaaata tcgcggacac
     2281 ccaggccctt cagggtgtcc tttgtcgcct tcaacatcgc ctcgccgaag cggacgatgc
     2341 tcgctccctc catggcgatg gatatgcccc gccccttttc cgtctcggta acggtgacga
     2401 tgcagtccat cgactcgagg gtccctgcct gggcagtttt catattctta cctccaggga
     2461 ccggg
//
LOCUS       DAOSRG010000182         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_39352_length_2436_cov_9.964721, whole genome shotgun sequence.
ACCESSION   DAOSRG010000182 DAOSRG010000000
VERSION     DAOSRG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39352_length_2436_cov_9.964721"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLM30_05855"
     CDS             complement(<1..442)
                     /locus_tag="PLM30_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOR54731.1"
                     /translation="MIVLIKDGAKEEQVRQLIERLESFGFQVHVSRGVVKTILGLVGN
                     TARLDIDLIRSMEIVQDVKRVQEPYKNANRKFHPENTVITVGGVTIGGGKPVLIAGPC
                     SVESEDQILEVARAVKRSGANMLRGGAFKPRTSPYSFQGLGEEGI"
     gene            835..1767
                     /locus_tag="PLM30_05860"
     CDS             835..1767
                     /locus_tag="PLM30_05860"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOR54732.1"
                     /translation="MVGTITIYGCGALGGRLAVQFHEAGVPVQCLSRQGAHLESIKAR
                     GLLFRDGAGGTRRVPLRVFDDPSECPPAELLVILVKSWQNADVAGNLPGLLKDDGFAL
                     TLQNGLGNVEALRDHVPGGRFLAGSITYGAYRPEPGAVFSGGDGLIRFAPVTPGMDAR
                     GALGLFREAGFNVELDEEPWRAIWAKVIINAGINPVAALTRYSNAQTLASPFARKVVE
                     SLCREAVSVANASGLDFDADEEWADLLEILRQTSGNKPSMLQDIEAGSRTEIEAISGE
                     IMRRGAAVGLETPFTTTLYNLVKALEAGAVAGGR"
     gene            1806..>2436
                     /gene="rarD"
                     /locus_tag="PLM30_05865"
     CDS             1806..>2436
                     /gene="rarD"
                     /locus_tag="PLM30_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOR54733.1"
                     /translation="MATTTITKRDASAGLKAALAAYGVWGLLPIYWKLLSGVPALAIL
                     AHRIVWSLVFVAGIMVLQGNRKELAAGFRNARVAALLFCSSLFIAGDWLLFIWAVNTD
                     QVLATSLGYYINPLVTIFFGFVFFRERVGRVQWLAIAIAAGGVLYQVVLHGNVPWVAL
                     GLALTFAFYGLIRKKVVIDSLPGLFAETVYLSLPALLFLVLGDVRHTGTF"
BASE COUNT          413 a          758 c          794 g          471 t
ORIGIN      
        1 ggatcccctc ctcgccaagg ccctggaagg agtaggggga ggtcctgggt ttgaaagcgc
       61 ctccccggag catgtttgcg ccggaacgct tcaccgcccg ggccacctcc aggatctggt
      121 cttccgactc tacggagcag ggccccgcga tgaggaccgg cttcccgccg ccgatggtga
      181 cgccgcccac agtgatcacc gtgttctccg ggtggaactt gcggttggcg ttcttgtagg
      241 gttcctggac ccgcttgaca tcctgcacaa tttccatgga ccggatcagg tcgatgtcca
      301 gccgggccgt gttccccacc agccccagga tggtcttcac cacccccctc gagacatgca
      361 cctggaaacc gaagctttcc agcctctcga tcagttgccg tacctgctcc tctttcgcgc
      421 cgtcctttat caatacgatc atgccgccat ctccagactt ccagggattt ttatataaaa
      481 aaaggtcccg cggcgtcgac cgcaggacct ttagttgtct ggttttgaag aagggggaaa
      541 cggttaaata tcaggggtcc ttgtcagtca ggcgccaaca tgctgtacct acgtaatagg
      601 tatagctgta ataggcgtaa tacctgaccg agcgagtgga acggaggacc gatgacatca
      661 gccgcacctc tttgggtcat tatttcggaa atttgggtca acataccacg ctggtctcac
      721 ccctgtcaag ttcacttttc tccacccctg ccgcggagag tgtatgcaac gtagcccctt
      781 ggaggatata atgaaaccac ggagcgggtg acggagcacg gcccgcccgc ggaggtggtt
      841 gggacgatca cgatctatgg gtgcggagcc ctgggcgggc gcctcgctgt gcaattccac
      901 gaggcggggg tgcccgtgca gtgcctgtcg cggcaggggg cccatctcga gagcatcaaa
      961 gcccgggggc tccttttcag agacggcgcc ggcgggacgc ggagggtccc cctgcgggtt
     1021 ttcgacgacc cttcggagtg cccccccgcc gagttgctgg tgatactggt caaatcgtgg
     1081 cagaacgccg acgtggcggg gaacctgccg gggcttttga aggacgatgg tttcgcgctg
     1141 accctccaga atggcctggg gaacgtggag gccctgcggg accacgtacc gggggggagg
     1201 ttcctcgccg ggtcgataac ctacggtgcc tacaggccgg agcccggggc cgttttcagc
     1261 ggcggcgacg gcctcatccg tttcgctccc gtcacgcccg ggatggacgc ccggggggcg
     1321 ctcgggctct tcagggaggc cgggttcaac gtcgaactcg acgaggagcc ctggcgggcg
     1381 atatgggcca aggtgatcat aaacgccggc atcaaccccg tggcggcgct gacgcgatac
     1441 tccaacgccc agaccctggc ctcgcctttc gccaggaagg tcgtggaatc cctctgccgg
     1501 gaggcggtct ccgtcgccaa cgcgtcgggc ctggactttg acgccgacga ggagtgggcc
     1561 gacctcctgg agatactcag gcagacgtcg ggcaacaagc cgtcgatgct ccaggatatc
     1621 gaggcgggga gcaggaccga gatagaggcc atcagcggtg agatcatgag gcggggcgcg
     1681 gcggtgggcc ttgaaacgcc cttcacgacc accctctaca acctcgtgaa ggccctggag
     1741 gccggcgccg tggccggcgg ccggtgacgg cggcccaagg tcgggcttga gaaggaagga
     1801 tcgttatggc gacaacaacc atcactaaaa gggatgcctc ggcggggctc aaggccgccc
     1861 tcgcggccta cggggtctgg ggcctgctcc cgatctactg gaagcttttg agcggcgtgc
     1921 cggcgctggc catcctggcc cacaggatcg tctggtcgct ggtcttcgtg gcggggatca
     1981 tggtcctcca gggcaaccgg aaggaactgg cggcgggctt ccggaacgcc cgcgtcgcgg
     2041 cgctgctctt ttgcagcagc cttttcatag ccggcgactg gctgctattc atctgggccg
     2101 tcaacacgga ccaggtgctg gcaacgagcc tgggttacta catcaacccc ctggtcacga
     2161 tcttcttcgg tttcgtcttc ttccgcgaga gggtgggcag ggtccagtgg ctcgccatag
     2221 ccatcgccgc cgggggggtg ctgtaccagg tcgtccttca cgggaacgtc ccctgggtgg
     2281 ccctggggct ggcgctgacc ttcgccttct acggcctcat caggaagaag gtggtcattg
     2341 attccctgcc ggggcttttc gccgagacgg tctacctttc cctgccggcg ctgctcttcc
     2401 tggtcctcgg cgatgtccgc catacgggga ccttca
//
LOCUS       DAOSRG010000183         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_39461_length_2431_cov_8.719697, whole genome shotgun sequence.
ACCESSION   DAOSRG010000183 DAOSRG010000000
VERSION     DAOSRG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39461_length_2431_cov_8.719697"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLM30_05870"
     CDS             <1..309
                     /locus_tag="PLM30_05870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR54734.1"
                     /translation="APHRGVDYRAAEGTPVKAAADGTVVLSADHYFAGRCVYLDHGGG
                     VVSVYLHLSKRLVEEGDVVSAGDVIGLSGRSGRVTGPHLHFGLCLQGMMVNPEPLLGR
                     "
     gene            complement(325..2277)
                     /locus_tag="PLM30_05875"
     CDS             complement(325..2277)
                     /locus_tag="PLM30_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR54735.1"
                     /translation="MIRCSRVTLTLGGRRVYDRMTLAVPPGARVGVVGDNGAGKTTFF
                     RLIAGEVSPDEGEVSLPSRWRLGYLPQDLVEVGDGPLLQLLKDKAGITRVERELSEVR
                     HRLSEPGTRPGERATLLARHERLVHAYEALDGYGFEAMAAKVLNGLGFRREDLGREAG
                     EFSGGWKMRVSLASILLSRPDVLLLDEPTNHLDTESMEWLEGYLASYRGTLMAVSHDR
                     RFLDKLTTSTVEVSGAGARSYPGNFSQYIQKRSLELEAAEKAAREQQKEIARTAAFIE
                     RFRYKASKAAQVQSRIRSLAKVERIDPGDPSRRVSMRFPPSPRSGLEVIKAVGVGHSY
                     GEKTVLSGVDLTVRRGERVALVGVNGAGKSTLTRLLSGREKPTRGGVTLGHNVKSAFF
                     SQESSENLDYGRSVWEEIRNAPSPCAEPQRRNLLGCFLFSGGDIEKPVRALSGGEKSR
                     LALLKILLRETNLLILDEPTNHLDMATRDLFQEALLGYGGTIVIVSHDRDFLDDLVTR
                     VIEIREGKVRDYPGNYSYFIEKRAEKEARGDLPTRGGSPSEGASEADPRERKRLEAER
                     RNRLYRARKKYLEDLGPLEAMIEKAEERVAAIDRSLSEPETLADPERIRGLLLERADL
                     QKKLLPMLERWEELMGSLEEAAREAR"
     gene            complement(2282..2416)
                     /locus_tag="PLM30_05880"
     CDS             complement(2282..2416)
                     /locus_tag="PLM30_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR54736.1"
                     /translation="MGFKSVSGIPQLMVGSTTTNVARHAHCSVLIYRPGFDPFEETPR
                     "
BASE COUNT          357 a          804 c          817 g          453 t
ORIGIN      
        1 gccccccaca ggggcgtgga ctaccgcgcc gccgagggca cccccgtcaa ggcggccgcc
       61 gacggcacgg tggtcctatc ggcggaccac tacttcgccg ggcggtgcgt ctatttggac
      121 cacggcgggg gagtggtgtc ggtctacctt cacctctcga aacggctggt ggaggagggc
      181 gacgtcgttt cagcggggga tgtgatcggg ctcagcggca ggagcggcag ggtcacgggg
      241 ccccaccttc acttcggcct ctgcctgcag ggcatgatgg tgaacccgga gcccctcctg
      301 gggaggtagg ggctgccttc ggactcaccg cgcctcccgg gccgcttctt caagggaacc
      361 catgagctcc tcccaccgct ccagcatcgg gaggagcttt ttttgcaggt ccgcccgttc
      421 cagcagcagg cccctgatcc tttccgggtc ggccagggtt tcaggttcgg agagggaccg
      481 gtcaatggcg gcgaccctct cctcggcctt ttctatcatc gcttcgaggg gtcccaggtc
      541 ctcgaggtac ttcttcctgg cccggtaaag cctgttcctc cgctcggcct cgagcctctt
      601 cctctcccgg gggtcggcct cggaggcacc ttcagagggg gagccgcccc gggtcgggag
      661 gtctccccgg gcttcttttt cagccctctt ttctatgaag taggagtagt tgcccgggta
      721 gtcgcggacc ttcccctccc tgatctcgat gaccctggtc accaggtcgt ccaggaagtc
      781 cctgtcgtgg gagacgatca cgatggtgcc gccgtagccc agtagggcct cctggaagag
      841 atccctcgtg gccatgtcca ggtggttggt gggctcgtcc aggatcagca ggttcgtctc
      901 cctgaggagg atcttcagca gggccagcct ggacttttcg ccccccgaaa gggcgcgcac
      961 cggtttttcg atgtcgccgc cggaaaaaag aaagcagccg aggaggttcc tccgctgggg
     1021 ctccgcgcag ggggaggggg cgttccttat ctcctcccag acggaccggc cgtagtcgag
     1081 gttttcggag ctctcctggg agaagaaggc gcttttcacg ttatgtccca gggtgacgcc
     1141 gccccgggtg ggcttttccc gcccgctgag cagcctcgtc agggtggatt tcccggcgcc
     1201 gttaaccccc accagggcca cccgctcgcc ccgccggacg gtgaggtcca cgccggaaag
     1261 gacggtcttt tcgccgtagg agtggcccac gccgacggcc tttatcacct cgaggccgct
     1321 cctgggcgac ggcgggaacc tcatggaaac cctccgtgag gggtctcccg ggtctatcct
     1381 ctcgaccttc gccagcgaac ggatcctgct ctggacctgg gcagccttag atgccttgta
     1441 gcggaagcgc tcgatgaagg cggccgtcct ggcgatctcc ttttgctgct cccgggcggc
     1501 cttttcggcc gcctccagtt ccagggaccg cttctggatg tactgggaaa agttgcccgg
     1561 gtaggaccgc gccccggcgc cggagacctc cacggtggag gtggtgagct tgtccaggaa
     1621 ccgccggtcg tgggaaacgg ccatcagggt gccccggtag gaggccaggt acccttccag
     1681 ccactccatg ctctccgtgt cgaggtggtt ggtgggttcg tccaggagga ggacatcggg
     1741 ccgggagagc aggatcgacg ccagggaaac ccgcatcttc cacccccccg agaattcgcc
     1801 cgcctcgcgg cccaggtcct cccggcggaa gccaagccca ttcaggacct tggccgccat
     1861 ggcctcgaag ccgtagccgt ccagggcttc gtaggcgtgg accagccttt cgtgccttgc
     1921 caggagggtc gccctctccc cggggcgggt ccccggttcg gagagcctgt ggcgcacctc
     1981 ggagagctcc ctttcgacgc gggttatgcc ggccttgtcc ttgaggagtt gcaggagggg
     2041 gccgtcgccg acctcgacga ggtcctgggg gaggtacccc aggcgccacc gggacgggag
     2101 ggagacctcc ccctcgtcgg gggagacctc cccggcaata aggcggaaaa aggtggtctt
     2161 gccggccccg ttgtcgccga cgacccccac ccgggctccc gggggtaccg cgagggtcat
     2221 cctgtcgtag accctccgcc ccccgagggt gagggtgaca cgggagcagc gtatcacggt
     2281 gtcaccgggg cgtttcctcg aaggggtcga aaccgggccg gtatatcagc accgaacagt
     2341 gggcgtgccg ggctacgttc gtggtggtgc tgcccaccat caactgcggt atgccgctga
     2401 cgctcttgaa gcctatgacg atcaggttga a
//
LOCUS       DAOSRG010000184         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_39941_length_2411_cov_12.504244, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000184 DAOSRG010000000
VERSION     DAOSRG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39941_length_2411_cov_12.504244"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2411)
                     /gene="rpoC"
                     /locus_tag="PLM30_05885"
     CDS             complement(<1..>2411)
                     /gene="rpoC"
                     /locus_tag="PLM30_05885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR54737.1"
                     /translation="IPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRKLQELRAP
                     EIIIRNEKRMLQESVDALIDNGRMGKAVLGAGNRPLKSLSDLLRGKKGRFRQNLLGKR
                     VDYSGRSVIVIGPNLKIHQCGVPKQMALELFKPFVINKLVDQGLSPNVKSAKRAIERG
                     REDVWGILEEVIKGHPVLLNRAPTLHRLGIQAFEPVLMEGKALRLHPLVCTAFNADFD
                     GDQMAVHVPLSIEARVEAQTIMLSARNLLSPASGRPVVTPTQDIVLGIYYVTAMLEGR
                     KGEGMSFLSIEDVLSALDFDVVDINSKIQLKFRGEWITTSPGRALFNSILHPDLRYIN
                     KQMGKKSLGALIDAAYDRVGQEALVDMLDKIKELGYQWSTVSGISFGLGDVVIPHQKK
                     DIVNEALSKEEVLSSQYEMGVLTEDEYLRQKETLWSEASREAADAVLANMDVTNPIRM
                     MMESGARGSKSQVAQMAGIRGLMADPSGKIIDYPIVSNFREGLNSLEYFISTHGARKG
                     LADTALRTAKSGYLTRRLVDVAQDLIITAEDCGTDMGVCIRPLLQDGKMIISLSERII
                     GRTNLRDIVNPETGEKIAASGELIDAEKAGQIEKAGLEEVWVRSPLTCALKDGICRQC
                     YGMDLSSREKVLLGEAVGVVAAQSIGEPGTQLTMRTFHTGGVRITGEDITQGLPRIEQ
                     LFEVRRPKKVAFLSDRDGFVSEIREMEGKRKVFISSEVDGVESRSSYNIPASQPLLVE
                     EGQPVAKGQALTDGHVDPQQLLEIEGLEAVQNYLVDNIQSVYLSQGVSVSNKHIEVIL
                     RKVAPV"
BASE COUNT          385 a          753 c          759 g          514 t
ORIGIN      
        1 acgggcgcga ccttccttag gatgacctcg atatgcttgt tgctgacgga gacgccctgg
       61 gagaggtaga ccgactggat attgtccacc aggtagttct ggaccgcctc caggccttcg
      121 atctcgagca attgctgcgg gtcgacgtgc ccgtcggtca gggcctgccc cttcgcgacg
      181 ggctgtccct cctcgacgag gaggggctgc gacgccggga tgttgtagga ggatcgggac
      241 tccacaccat ccacctcgga ggatatgaag acctttctct tgccctccat ctcccggatc
      301 tccgaaacga acccgtcccg gtcggagagg aaagccacct ttttgggcct ccggacctcg
      361 aagagctgtt ctatcctggg aaggccctgg gtgatgtcct caccggtgat gcggacgccg
      421 ccggtatgga aggtccgcat ggtcagctgg gtcccgggct cccctatgga ctgggccgct
      481 acgaccccga cggcctcgcc caggagtacc ttctcccgcg acgacaggtc catgccgtag
      541 cactgccggc atatgccgtc cttgagggcg caggtcaggg ggctcctcac ccagacctcc
      601 tccaggccgg ccttttcgat ctgacccgcc ttctcggcgt cgatcagctc cccggaggcc
      661 gcgatctttt cccccgtctc ggggttcact atatccctca ggttggtcct gccgattatc
      721 ctctcgctga gggagatgat catcttgccg tcctggagca ggggccttat gcagacaccc
      781 atgtcggtgc cgcagtcctc ggcggttatg atcaggtcct gggcgacatc gacaaggcgc
      841 cgggtaaggt aaccggactt ggccgtcctg agggcggtat cggccagccc ctttctcgcg
      901 ccgtgggtgg atatgaagta ttccaggctg ttgaggccct cacggaagtt agagacgatg
      961 gggtagtcga tgatcttccc tgacgggtcg gccatgagcc cccggatgcc ggccatctgt
     1021 gccacttggc ttttgcttcc acgggcgccg gattccatca tcatcctgat ggggttggtg
     1081 acatccatgt tggccaggac cgcatcggcc gcctcccggg aggcctccga ccagagggtc
     1141 tccttctgcc ggaggtattc gtcctcggtg aggaccccca tctcgtactg ggacgagagg
     1201 acctcctcct tggagagggc ctcgttaacg atgtcctttt tctgatgggg tatgacgacg
     1261 tcgccaaggc cgaagctgat gccgctgacg gtcgaccact ggtaccccag ttcctttatc
     1321 ttgtccagca tgtccaccag ggcctcctgc cccacccggt cgtaagcggc gtcgatgagc
     1381 gcgcccaggg atttcttccc catctgcttg tttatatagc gcaggtcggg gtggagtatg
     1441 ctgttgaaga gggcccggcc gggggaggtg gtgatccatt cacccctgaa cttgagctgg
     1501 atcttgctgt tgatgtccac cacgtcgaaa tcgagggcgg atagaacgtc ctcgatggag
     1561 aggaagctca tcccttcgcc cttcctgccc tcgagcatgg ccgtgacata gtagatcccc
     1621 agcacgatgt cctgggtggg cgtgacgacg ggcctcccgc tcgccggcga aaggaggttc
     1681 ctggcggaga gcattatcgt ctgggcctcc acgcgggcct cgatggacag cggcacgtgg
     1741 accgccatct ggtcaccgtc gaagtcggcg ttgaaggcgg tgcacaccag ggggtggagt
     1801 ctcagggcct taccttccat gaggaccggt tcaaaggcct ggatccccag ccggtgaagc
     1861 gtcggcgccc tgttgaggag cacggggtgg cccttgatga cctcctccag tatgccccac
     1921 acgtcctcgc gcccccgctc gatggccctc ttggcgctct tgacgttggg cgagaggccc
     1981 tgatccacga gcttgttgat gacgaagggt ttgaagagtt ccagggccat ttgcttcggg
     2041 acgccgcact ggtggatctt caggttcggt ccgatgacga tgacggacct gcccgagtag
     2101 tcgaccctct tgccgagcag gttctggcgg aagcggccct tcttgccccg caggaggtcg
     2161 gaaaggctct tcaggggcct gttcccggca ccgagcacgg ccttgcccat cctcccgttg
     2221 tcgatcaggg catcgacgga ctcctggagc atcctcttct cgttacggat gattatctcc
     2281 ggcgccctga gctcctgcaa cttgcggagc ctgttgttgc ggtttataac cctgcggtag
     2341 aggtcgttga ggtccgaggt ggcgaaacgg ccgccgtcca gctggaccat gggcctcagg
     2401 tcgggcggga t
//
LOCUS       DAOSRG010000185         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_40330_length_2395_cov_11.828632, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000185 DAOSRG010000000
VERSION     DAOSRG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40330_length_2395_cov_11.828632"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PLM30_05890"
     CDS             <1..987
                     /locus_tag="PLM30_05890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOR54738.1"
                     /translation="NDTFPTAMHIAAVLQVEDLLLPSLKRFKKTLEAKSSKYKDLVKT
                     GRTHLQDATPLTLGQEISGWVRMLERSEEMLTSSIEYLKDLGLGGTAVGTGLNAAEGY
                     DVEVAAQIGALTGKKFKTAVNKFHSLTSKDEVVFVHGAMKALAADLLKIVNDIRWLAS
                     GPRCGIGELIIPANEPGSSIMPGKVNPTQCEAVSMVVAQVLGNDVTVGFAASQGNFEL
                     NVYMPVMIYNFLQSGRLLADAMKSFNDHCAAGLEADEEKVTHFVQNSLMNVTALNPYI
                     GYDKSAEIAKKAHNDGSTLKEAALALGYLSAEEFDRYMDLKAMCAPKTVKVK"
     gene            1104..>2395
                     /locus_tag="PLM30_05895"
     CDS             1104..>2395
                     /locus_tag="PLM30_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOR54739.1"
                     /translation="MPSKEELLAKAQKPAEDAMKLHPFYKGKMQTAPKCCIRDIGDFA
                     IWYTPGVAAPCKAIKEDVNLSYEYTNRGNMVAVVSDGTRVLGLGDIGPEAGMPVMEGK
                     SLLFKYLGGVDAVAICLDTKDPDKIIEAVRLLAPSFGGINLEDISQPKCFYVLDELRK
                     DCKIPVWHDDQQGTAAVNLAGLFSGLKLTGKKIDEVKVRFFGAGAASIATARILISAG
                     VRPENVIMADSKGTLHKGRTELKDIPYDVKWHFARTTNPEGIQGGPEVLFKDADVVYC
                     YTKPGPDVVKKEWVRLMAKDAMLFACANPIPEIWPWDAKEAGARIVATGRSDFPNQIN
                     NSLGFPGIFRGTLDVQATTITDEMCIAAAETLSKCAEKKGLSEDYLIPTMDQSWVYPE
                     VAASVGMKAIEQGIARKVMTRDELLEIATEKIQYAQDMT"
BASE COUNT          550 a          728 c          736 g          381 t
ORIGIN      
        1 aacgacacct tcccgacggc gatgcacata gcggcggtcc tccaggtcga ggacctgctt
       61 ctcccctcgc tgaagagatt caaaaagacc ctcgaggcca agtccagtaa gtacaaggac
      121 ctggtgaaga ccggaaggac ccatcttcag gatgccaccc cccttacgct cggccaggag
      181 atcagcggct gggtgaggat gctcgagcgc tccgaggaga tgctgacttc cagcatcgag
      241 tacctcaagg atctcggcct gggcggcacg gcggtgggca ccggtcttaa cgccgccgag
      301 ggttacgacg tggaggtggc cgcccagatc ggcgcactga cgggaaagaa attcaagacc
      361 gccgtcaaca agttccactc cttgacgagc aaggacgagg tagtcttcgt ccacggcgcc
      421 atgaaggccc tggccgccga ccttctgaag atagtcaacg acatacgctg gctggcctcg
      481 ggcccccgtt gcggcatagg ggagctcatc atccccgcca acgagccggg cagctccatc
      541 atgcctggca aggtgaaccc gacccagtgc gaggccgtct ccatggtggt ggcccaggtg
      601 ctcggcaacg acgtcaccgt gggatttgcc gcgagccagg gcaacttcga actcaacgtc
      661 tacatgcccg tcatgatata caacttcctc cagtcgggaa ggctcctggc cgacgccatg
      721 aagtccttca acgaccactg cgccgcaggg ctcgaggccg acgaggagaa ggtcacccac
      781 tttgtgcaga attccctcat gaacgtcacg gcgctcaacc cctatatcgg ctacgacaag
      841 tccgccgaga tagccaagaa ggcccataac gacggttcca ccctcaagga agccgccctg
      901 gccctgggtt acctcagcgc cgaggaattc gaccgctaca tggacctgaa ggccatgtgc
      961 gccccgaaga cggtcaaggt gaagtaacag accctggcgc ggaaaggttc aacaacggga
     1021 tgaacaggcc cctcctgccg aaaaagaggc aggaggggcg ttaacgggac cggacggaac
     1081 gaacactaag gaggcgttaa agcatgccga gtaaggaaga acttttggcg aaggcgcaga
     1141 aaccggcgga ggacgcgatg aaacttcacc ccttctacaa ggggaagatg cagacggcgc
     1201 ccaaatgctg catcagggac ataggcgatt tcgcgatctg gtacaccccg ggtgtggccg
     1261 cgccctgcaa ggcgataaag gaggacgtga acctctccta cgagtacacc aaccgcggca
     1321 acatggtcgc cgtcgtcagc gacggcacga gggtcctcgg cctgggtgac atcgggcccg
     1381 aggcgggtat gccggtcatg gagggcaaat cgcttctttt caaatacctg ggcggcgtcg
     1441 atgccgtggc catctgcctg gacacgaagg accccgacaa gatcatcgag gccgtaaggc
     1501 ttttggcgcc gtccttcggc ggcatcaacc tcgaggacat atcgcagccg aagtgctttt
     1561 acgtactgga cgaactgcgc aaggactgca agatccccgt atggcacgat gaccagcagg
     1621 gcaccgctgc cgtcaacctg gcgggccttt tcagcggttt gaagttaacg ggcaagaaga
     1681 tcgacgaggt caaggtgcgt ttcttcggcg ccggggcggc cagcatagcc acggccagga
     1741 tcctcatcag cgccggcgtg aggcccgaga acgtgatcat ggccgacagc aaggggacgc
     1801 tccacaaggg gcgcacggaa ctcaaggaca taccctacga cgtgaagtgg cacttcgcgc
     1861 ggaccaccaa ccccgagggg atccagggcg ggcccgaggt tcttttcaag gacgccgacg
     1921 tggtctactg ttacacgaaa cccggccccg acgtggtcaa gaaggaatgg gtccgattga
     1981 tggccaagga tgccatgctc ttcgcctgcg ccaacccgat ccccgagatc tggccctggg
     2041 acgcgaagga agcgggggcc cggatagtgg ccaccggaag gtctgacttc ccgaaccaga
     2101 taaacaactc cctcggtttc ccgggcatct tcaggggcac ccttgacgtg caggccacga
     2161 ccattaccga cgagatgtgc attgccgcag ccgagaccct ttcgaaatgc gccgagaaga
     2221 agggccttag cgaggactac ctgatcccga cgatggacca gtcgtgggta taccccgagg
     2281 tggcggcctc ggtcggcatg aaggccattg agcagggcat agcccgcaag gtcatgacta
     2341 gggatgaact tttggagatc gccaccgaaa agatccagta cgcccaggac atgac
//
LOCUS       DAOSRG010000186         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_40331_length_2395_cov_10.970513, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000186 DAOSRG010000000
VERSION     DAOSRG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40331_length_2395_cov_10.970513"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLM30_05900"
     rRNA            <1..226
                     /locus_tag="PLM30_05900"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            286..362
                     /locus_tag="PLM30_05905"
     tRNA            286..362
                     /locus_tag="PLM30_05905"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Ile,seq:gat)
     gene            371..445
                     /locus_tag="PLM30_05910"
     tRNA            371..445
                     /locus_tag="PLM30_05910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Ala,seq:tgc)
     gene            892..1428
                     /locus_tag="PLM30_05915"
     CDS             892..1428
                     /locus_tag="PLM30_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR54740.1"
                     /translation="MKDLMELLRERRSIRRYQDRPLSDEQVAALEEAVLRSPTGRDTR
                     SWEFVFVDDRELLNRLAATRGSNSAFLGGASLGIVVLGNDKVTDTWVEDSSLAAIIAQ
                     LAAVGLGLGSCWSQVRNREHAPGETAEDYVRGLLGIPDDLRVLCVIAVGHPSEEKRPT
                     PKGGLSFRKIHRNRFKPA"
     gene            complement(1449..>2395)
                     /locus_tag="PLM30_05920"
     CDS             complement(1449..>2395)
                     /locus_tag="PLM30_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR54741.1"
                     /translation="ESGFRGIKWYAANEEAPVAGFTPDIPEGIVHAEKGGLHIRIDVP
                     VGANDATRLVSFQAGEAVNIVPDRCEAVFAGDPRVLDDWAEVLRGAVEAMPLGGEVLF
                     EKGALTVRAFGKAAHASLPENGENAIAALCRLIDATPPPGGPFEAFKVLSRLLEDPRF
                     GSSLGIAMSDDVSGPLTCNPGLASFSKDVLSVSLDIRHPVTIKGDLVAERLKERLAPF
                     GLVPVETSRYSPLFVPKDSPLIRDLSEAFFEATGKHPEVMAIGGGTYAKALPNVVAFS
                     PFPAGLMDLAHQADEHIGIEDLLSSVLVLKTAMLKLAR"
BASE COUNT          466 a          669 c          774 g          486 t
ORIGIN      
        1 acaccgcccg tcacaccacc cgagttgggt gcacccgaag ccggtggccg aacccggcac
       61 tcaactgggg agagcgtcct cttttttaag gcacggtagt gctgtaagga gtggaggttc
      121 tttcttgttg agtgccgggg cggagccgtc taaggtgtgc ctggtaaggg gggtgaagtc
      181 gtaacaaggt agccgtaccg gaaggtgcgg ctggatcacc tcctttctag ggagtatcat
      241 aagaggtacc ccatgggttt ttgtacgctg tatctttttt cccgagggcc gatagctcag
      301 gtggctagag cacacggctg ataaccgtga ggtcggaggt tcgaatcctc ctcggcccac
      361 cagccttttc ggggatgtag ctcagtggga gagcgcctgc cttgcacgca ggaagtcgtc
      421 ggttcgaatc cgatcatctc caccaaccaa ataaatgcgg tcctaaatgc ggtcggaggg
      481 accgcatttt tcaattggat cggcaagggt cctggttttc agaacctcct gtcggcgagg
      541 gtttcactct tttcgccctt ccccccggcg atagcgattc tgtttctcct gccccttgat
      601 tttcttttga ggttgttgta ttattctatt atcggggaga agtgccctcg aagtcgggtt
      661 ttccggcttg aaaagcggtt tgggaagtga agaagcagcg gcagaagtat caagtttcaa
      721 attgtaagtg aagtgctcca gtaaagagga acggttttta acggaacagg gagtggttgc
      781 ttgaagtgtc tctgcgccgg aaaacccgac caaggtaata taggggtggc ggaagccgcc
      841 cctttttcat ttccccggca gacgggggct ctttcaaaag gagtggtgga aatgaaggac
      901 ctgatggagc tgttgagaga acgaagaagt atcaggcggt accaggaccg tcccctttcg
      961 gacgaacagg tggctgccct ggaggaggcc gtgctccgga gtcccacggg gagagacact
     1021 aggtcttggg agttcgtttt cgtcgatgac cgggaactgc tgaacaggct tgccgccacc
     1081 agggggagca attcggcctt cctcgggggc gcgtccctgg gcattgtcgt cctgggaaat
     1141 gataaggtta ccgacacctg ggtcgaggac tcgtccctgg cggcgatcat agcccaactg
     1201 gccgccgtag gcctgggcct cgggtcgtgc tggtcccagg tccggaacag ggagcacgcc
     1261 cccggcgaga cggccgagga ttatgtcagg ggtcttttgg gcatccccga tgatctcagg
     1321 gtgctgtgcg tcatcgccgt gggccatccc tccgaggaga aacgccccac cccaaaagga
     1381 ggcctgtcct tccggaagat ccacaggaat cggttcaagc cggcctgaga cggcgggtga
     1441 gcgggacctc acctcgccag cttgagcatg gcggtcttga ggaccaggac ggaggacaaa
     1501 aggtcctcga tcccgatgtg ttcatcggcc tggtgggcca ggtccataag gcccgcgggg
     1561 aaaggggaga aggccactac gttcgggagg gccttggcat aggtcccgcc gccgatagcc
     1621 atcacttccg ggtgcttccc cgtagcctcg aaaaaagcct cgctgaggtc cctgatcagg
     1681 ggtgaatcct tggggacgaa gagggggctg taccttgagg tctccacggg gacgaggccg
     1741 aacggggcca gccgttcctt cagcctttcc gccaccaggt cgcccttgat cgtcactgga
     1801 tgcctgatat cgagggatac cgagaggaca tcctttgaaa aagaagccag tccggggttg
     1861 cacgtcagcg ggccggagac gtcatcgctc atggctatcc ccaggctgga accgaacctc
     1921 gggtcttcca gcagccgtga taagaccttg aaggcctcga agggtccgcc gggagggggg
     1981 gtggcatcga tcagacggca gagggccgcg atggcgttct cgccattttc agggaggctc
     2041 gcgtgggccg ccttgccgaa agcccttacg gtaagggccc ccttttcgaa aagcacctcg
     2101 ccgcccagag gcatggcctc gacagcgccg cggaggacct ccgcccagtc atcaaggacc
     2161 cgcgggtcgc cggcgaaaac ggcctcgcac cggtcgggga caatatttac ggcctccccc
     2221 gcctggaagg agaccagccg ggtcgcgtcg ttggctccga caggtacatc gatccggata
     2281 tggaggccgc ccttctcggc atgaacgatc ccctccggta tatcaggggt gaagcccgct
     2341 acgggggctt cctcgttggc ggcgtaccac ttgatgcccc ggaagccgct ttcct
//
LOCUS       DAOSRG010000187         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_40355_length_2394_cov_7.485678, whole genome shotgun sequence.
ACCESSION   DAOSRG010000187 DAOSRG010000000
VERSION     DAOSRG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40355_length_2394_cov_7.485678"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLM30_05925"
     CDS             <1..259
                     /locus_tag="PLM30_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54742.1"
                     /translation="GAFLAGLAGGFVLSLVGVPCMTVGIGIYLPVAISLAAGAGGIIR
                     AVTGRLFPRGEDDLTLVASGFLGGEGIAGVLFAFWKVVTLG"
     gene            272..1192
                     /locus_tag="PLM30_05930"
     CDS             272..1192
                     /locus_tag="PLM30_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOR54743.1"
                     /translation="MWRIGHAQDHEKGTGCTVLVFPDGAAASCDIRGGAPGTRETALL
                     APECTVDKINALVLTGGSARGLSAADGVMRYCEERGWGFNSGFGMIPIVPAACLFDLG
                     CGEPSAWPEPSMGMEACLGARPVEESPMGNYGAGTGATVGKYSPLGKAMKSGFGWARK
                     VSGELEVCAVTALNAFGAVRDLAGGFMAGALDGERIIDPGSMSGAGDVWNEWGRNTTL
                     AAIVTNARLDKAACRRVAIMGQAGLAGALFPFNTPFDGDTVFCVATGETSSSTVLVGW
                     LAASALEEAARNAVLHARSAFGFVSFSDLR"
     gene            1304..1939
                     /locus_tag="PLM30_05935"
     CDS             1304..1939
                     /locus_tag="PLM30_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR54744.1"
                     /translation="MKIRVFLADDHPLTRAGLRDYIAKEKDLELVGEAGDGLEAWRVI
                     EKEKPDVALLDIRMPGENGVALARRIKASSLPVKPVMLTSFDAQQYVMASLRAGARGY
                     VLKTVTPEELARAIRVVARGGLYLDGEVARSMEDGEFAYEALSAREREVLLHAARGLS
                     SKEIAGVLIISERTVQTHLASIYDKLGARNKTEAMLLALKYGIVALEELIE"
     gene            1936..>2394
                     /locus_tag="PLM30_05940"
     CDS             1936..>2394
                     /locus_tag="PLM30_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOR54745.1"
                     /translation="MRRHLLILLTVAIMIPSFSALLLGGAGLIQHERAMERVARSYVV
                     DIAESIASRLDLSWNRTRPSPFGDRERFMRLRQLTWGLSMPGWVAVVDRQGNILLSTG
                     DSDALPLLWDRGLPIGSALEVQSREGEKFTLAVYPAGETGWYVVAAVSWDK"
BASE COUNT          405 a          677 c          840 g          472 t
ORIGIN      
        1 gggagccttc cttgccggcc tggcgggagg gttcgtccta tccctggtgg gtgtcccctg
       61 catgaccgtg ggcatcggga tatacctgcc cgtggcgata tccctcgccg cgggggccgg
      121 cggtattatc agggccgtga ccgggcggct tttcccgcgc ggggaggacg acctcaccct
      181 tgttgcctcg gggttccttg ggggagaggg gatcgccggg gttcttttcg ccttctggaa
      241 ggtcgtcact ttgggttgag gggtgatctt gatgtggcgg ataggacatg cccaggacca
      301 cgagaagggg acgggctgta ccgttctggt cttccccgat ggggctgccg cctcctgcga
      361 tatcaggggt ggcgcgcctg gtacgagaga aacggcgctt ttggcaccgg aatgcactgt
      421 cgataagatc aacgccctgg tgctcaccgg tgggagcgcc cggggcctct cggcggccga
      481 tggggtcatg cggtactgcg aggagagggg ttgggggttc aattccgggt ttgggatgat
      541 ccccatcgtg cccgcggcgt gccttttcga tcttggctgc ggcgaaccat cggcgtggcc
      601 ggaaccctcc atggggatgg aggcctgcct gggggctagg cccgtcgagg agtcgcccat
      661 gggtaattac ggggccggga ccggggccac cgtgggcaag tattcccctt tgggcaaggc
      721 tatgaaatca ggtttcggct gggcaaggaa ggtgtcgggg gaactcgagg tgtgcgccgt
      781 cacggcgtta aacgcctttg gagctgtcag ggaccttgcc ggcgggttca tggcgggggc
      841 ccttgatgga gagcgcatca tcgaccccgg gtcaatgtcg ggggctgggg atgtgtggaa
      901 tgagtggggg aggaacacca ccctcgccgc catcgtaacg aacgcgaggt tggacaaagc
      961 cgcctgcagg agggtcgcca tcatggggca ggcgggactc gccggagccc ttttcccttt
     1021 caacactccc tttgacggtg atacggtctt ctgcgttgcc accggcgaga ccagttcctc
     1081 gacggtcctc gtggggtggc ttgccgcctc ggccctcgag gaagccgcca ggaacgcggt
     1141 actccacgcg aggtcggctt ttgggttcgt atccttctcc gacctccgct gaaaccgctc
     1201 aaacccagtc ggttatcggt aaggctgcgg atacgccgcc cggtccattt agggcatact
     1261 tgccggatag gggccgcctt tatggtccca ggaggtgcat tccatgaaga taagggtttt
     1321 cctcgccgat gatcaccccc tcacccgcgc cgggttgagg gattacatag cgaaggagaa
     1381 ggacctggag ctggtggggg aggccggtga cggtcttgag gcctggaggg tcatcgaaaa
     1441 ggaaaagccc gacgtggccc tgctggacat caggatgccc ggcgagaacg gcgtcgccct
     1501 ggccaggagg atcaaggcat cctcactccc ggtcaagccg gtgatgctca catccttcga
     1561 tgcccagcag tacgtcatgg cctcgctcag ggccggggcc aggggttacg tcctcaagac
     1621 cgttacgccc gaggagctcg cccgcgccat cagggtggtg gcccgggggg ggctttacct
     1681 ggatggggaa gtggcccgca gcatggagga cggggaattc gcctacgagg cgctgtcggc
     1741 aagggagagg gaggtcctcc tccatgcagc cagggggctg tccagcaagg agatcgcggg
     1801 cgttctcatc atcagcgaga ggacggtcca gacccacctt gcttccatat acgacaagct
     1861 cggggccagg aacaagaccg aggcgatgct gctggcgctt aagtacggca tcgtagcctt
     1921 ggaggaactg atcgaatgag gagacacctg ctcatccttc tcacagtggc catcatgata
     1981 ccctcctttt cggcgctcct cttagggggc gccggtctca tccagcacga gagggccatg
     2041 gagagggtcg ccaggtccta cgtggtcgac attgccgaga gtattgcctc gagactggat
     2101 ctgagctgga acaggacaag gccttccccc ttcggtgatc gtgagaggtt catgaggctc
     2161 aggcagctca cctggggcct ttctatgcca ggctgggtgg cggtagtgga caggcagggg
     2221 aacattcttt tgtccaccgg tgactccgac gcccttcctc tcctctggga cagggggtta
     2281 ccgatcggtt cagccctgga agtccaaagc cgggagggcg agaagttcac ccttgccgtt
     2341 tatcctgccg gggaaaccgg gtggtacgtt gtggccgccg tatcctggga taag
//
LOCUS       DAOSRG010000188         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_40358_length_2394_cov_6.516032, whole genome shotgun sequence.
ACCESSION   DAOSRG010000188 DAOSRG010000000
VERSION     DAOSRG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40358_length_2394_cov_6.516032"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLM30_05945"
     CDS             <1..275
                     /locus_tag="PLM30_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HOR54746.1"
                     /translation="ALSGIEIEDEKTEAMVEFFKELVGHFQGLESCDIEGMDPFFDPG
                     GSPYPLRGDEPVRWEDSDKALEAGALIEGTFFIVPPISGAGVPDES"
     gene            265..1734
                     /gene="gatA"
                     /locus_tag="PLM30_05950"
     CDS             265..1734
                     /gene="gatA"
                     /locus_tag="PLM30_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOR54747.1"
                     /translation="MSREPMDAAAIASAVAGRECSAVEVVSASLERIDAQEPRLNALI
                     TVLEDQALERAAFIDGQVASGNDPGPLAGVPVVVKDNLCTRGIRTTCGSRMLENWKPP
                     YDAAVVEKLEGAGAVIVGKSNMDEFAMGSSSEHSRFGPVSNPRDPSRVPGGSSGGSAA
                     SVAAGYVPIALGSDTGGSIRQPAAFCGVHGLKPTYGAVSRWGLIAFASSLDQVGPLSR
                     SVVDAALTMDVISGCDPRDSTSKGGGFPRFIEGALNPYVKGTRIGIVREFREGVLEQP
                     LAKAIEDAALALGESGCAMAEVSLEESVTFGLPCYYIISPAEASSNLARFDGTRYGLR
                     MDAQDFVSQLLLTRGAGFGDEVKRRILTGSFVLSSGYYDAFYATALKVRKKICAEFDR
                     AFTSADVILMPAVSSFPFKKGNFDDDPVGMYKSDLFTLPVNLAGLPGMTVFTGLERSG
                     LPTSVQLVAPRWQDQRLIKCAAVIEKKFGRPPVACGGCI"
     gene            1734..>2394
                     /locus_tag="PLM30_05955"
     CDS             1734..>2394
                     /locus_tag="PLM30_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOR54748.1"
                     /translation="MALTFETVIGLEVHVQLSTNSKLFCPCPTDYIGREPNTFVCPVC
                     LGLPGSLPSLNARAVQLGVKIALALGCFVSRVSRFDRKHYFYPDLPKAFQVSQLGLPL
                     AEKGAMPIFLGGLKKTVGITRLHLEEDAGKLLHGTPDGRLSGTSFTRVDYNRAGIPLA
                     EVVSEPDMSTPMEAREYVLSLRRIVRYLGASDGDMESGSLRVDVNVSQKCSDGRVGGR
                     VE"
BASE COUNT          439 a          711 c          756 g          488 t
ORIGIN      
        1 cggccctatc ggggatcgaa atagaggatg aaaagaccga agccatggtc gaatttttca
       61 aggagttagt cgggcacttt caaggtcttg agagctgtga catcgagggt atggaccctt
      121 ttttcgatcc tggcggttcg ccctaccccc tcaggggcga tgagccggta cgctgggagg
      181 acagcgacaa ggccctcgag gccggagctt tgatagaagg cacctttttc atcgtccctc
      241 cgatcagcgg agcgggggtc cccgatgagt cgtgagccta tggatgccgc cgccattgcc
      301 tccgccgtcg cgggtcgcga atgttcggcg gtggaagtcg tgtcggcctc gctggagagg
      361 atcgacgccc aggaaccccg actgaacgcc cttattaccg tcttggagga ccaggccctc
      421 gagagggccg ccttcatcga cggccaagtc gcctctggga acgacccggg gccgctggcg
      481 ggagtgccgg tcgtcgtgaa ggacaacctg tgcacccggg gcatcaggac cacttgcggg
      541 agcaggatgc tggaaaactg gaagcccccc tacgatgccg ccgtggtaga gaagctggag
      601 ggggcggggg cggtcatagt ggggaagtcc aacatggacg agttcgccat gggcagttcc
      661 tccgaacact cccgcttcgg ccccgtgtcg aacccacggg acccctccag ggtgccggga
      721 gggagttccg ggggaagcgc cgcgtcggtc gcggccggtt atgtgcccat cgccctcggc
      781 agcgacaccg gcggatccat ccgccagccg gcggccttct gcggggtcca cggccttaaa
      841 ccaacctatg gtgccgtcag ccgctggggc ctgatcgctt tcgcgtcctc cctcgatcag
      901 gtaggacctt tatccaggag tgtcgtcgat gcggccctca cgatggacgt tatttccggc
      961 tgcgaccccc gggactccac cagcaagggg ggagggttcc cccggttcat cgaaggggcc
     1021 ctgaaccctt acgtaaaggg gacgcggatc ggtatcgtcc gcgagttccg ggaaggcgtc
     1081 cttgagcagc ccctcgccaa ggccattgag gacgcggccc tggcgctcgg ggagtcgggt
     1141 tgcgccatgg ctgaggtctc gctcgaggag tcagtgacct tcgggttgcc ctgctactat
     1201 attatttccc cggccgaagc gagttccaac cttgctcggt tcgacgggac caggtacggc
     1261 ctcaggatgg acgcgcagga cttcgtctca cagttgctac tcaccagggg cgccggcttt
     1321 ggtgacgagg taaaaagaag gatactcacg ggttccttcg ttctcagttc cggctactac
     1381 gatgccttct acgcgacggc gctgaaggtg agaaaaaaga tctgtgccga gttcgaccgt
     1441 gccttcactt ccgctgatgt gatactcatg cccgccgttt cttcttttcc tttcaaaaag
     1501 gggaatttcg acgatgatcc tgtcgggatg tataaaagcg atctcttcac gctgccggtg
     1561 aatctcgcgg ggctccccgg catgacggtc ttcactggac tggaaaggtc cggcttgccg
     1621 acgagcgtac agttagtggc ccctcgttgg caggatcaaa ggcttataaa atgcgccgcc
     1681 gtgatcgaga aaaagttcgg gcgtcccccg gtcgcctgcg gggggtgcat ttgatggccc
     1741 ttacttttga aaccgtcata ggcctggagg tacacgttca actatcgacg aattcgaagc
     1801 tcttttgccc ctgcccgacg gactatattg ggcgcgaacc caacactttc gtctgtcccg
     1861 tttgcctggg tctccccggg tcacttcctt cgcttaacgc cagggccgtc caactcgggg
     1921 tgaagatcgc cctggcactg ggttgtttcg tttcgagggt atccaggttc gacaggaagc
     1981 actactttta ccccgacctc cccaaagcct tccaggtaag ccagttaggg ctgcccttgg
     2041 ccgaaaaagg cgccatgccc atcttcttag ggggactgaa aaaaaccgtg ggtatcacgc
     2101 ggctccatct cgaggaggac gcgggcaagt tgctccatgg gacccccgat ggcaggcttt
     2161 ccggcacctc ctttacccgg gttgattaca accgggcagg gatacccctc gcggaggtag
     2221 tctccgaacc ggacatgtcc accccgatgg aggccaggga gtacgtcctg tccctgcgaa
     2281 ggattgtgag gtacctgggc gcctccgatg gcgacatgga atcgggctcc ctcagggttg
     2341 atgtcaacgt ctcccagaag tgttccgacg gaagggttgg cggcagggtc gagg
//
LOCUS       DAOSRG010000189         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_40577_length_2384_cov_12.819665, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000189 DAOSRG010000000
VERSION     DAOSRG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40577_length_2384_cov_12.819665"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..302
                     /gene="secG"
                     /locus_tag="PLM30_05960"
     CDS             78..302
                     /gene="secG"
                     /locus_tag="PLM30_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOR54749.1"
                     /translation="MKVFLTVVHVIVSIAVMAVILMQQRKQGGFTGVFGGGTQADAAG
                     QWQRFTLLTKATIVLTAVFLATSLILVIVR"
     gene            320..1360
                     /locus_tag="PLM30_05965"
     CDS             320..1360
                     /locus_tag="PLM30_05965"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOR54750.1"
                     /translation="MGSPRLDRLSDVYAFEPGTARLFSATHEEILSGKTTDIYFVKTR
                     DILDACGRLDAPVVAEIFAKRGGVFAGIPEVVSLLEDTGVEILSLREGDTFAPDEVVA
                     RISGPYSAFGMYETVILGMLASSTGWATAARECVEAAGGRPVLSFGARHVHPAVSSVM
                     ERAAVVAGGCVGASCILGAKLAGIEPRGTIPHAAILIAGDTLKIARTYDDFLPEGEPR
                     IILVDTFHDEAEEALRIASALGERLSAVRLDTPSERGGVTPELVREVRRRLDMAGASH
                     VTITVSGGLNPERIRLLSEAGAASFGVGSYIAHATPRDMTMDIKVVDGVPVAKRGRIP
                     GITVNPSLEKAL"
     gene            1510..1941
                     /gene="rplM"
                     /locus_tag="PLM30_05970"
     CDS             1510..1941
                     /gene="rplM"
                     /locus_tag="PLM30_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR54751.1"
                     /translation="MAVNSSYMAKKEQVERKWYVIDATDKVLGRLAAQVAMILQGKNK
                     PTYTPHVDTGDFIVVVNADKVKITGKKLEKKYLYRHSSYKGSLKATSYGTFMEKYPDR
                     LFERVVKGMLPKNRLHFEKKLKVYAGPEHPHAAQKPEPLDI"
     gene            1972..2367
                     /gene="rpsI"
                     /locus_tag="PLM30_05975"
     CDS             1972..2367
                     /gene="rpsI"
                     /locus_tag="PLM30_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR54752.1"
                     /translation="MKRSEYYWGTGRRKAALARVRVRSGSGMLKVNERPADEYFPRSV
                     WQSQAFEALRTSGLEGKIDVFVNASGGGLTGQSGAVRLGLARALLKMDPGLKPALKRS
                     GLLTRDSRMVERKKFGQKGARGKRQFSKR"
BASE COUNT          480 a          744 c          769 g          391 t
ORIGIN      
        1 gggcgtatgc tccccggctg ttgtgtggta aaataagcct tcgtttcagg tcaaggatca
       61 tcaaggaggt gcaaggcgtg aaggttttcc tcaccgttgt tcatgtgatc gtctccatcg
      121 cggtgatggc cgtcatcctc atgcagcagc gaaagcaggg cggcttcacc ggcgttttcg
      181 gcggcggtac ccaggccgat gccgccggcc agtggcagcg gttcaccctg ctcaccaagg
      241 cgaccatagt gctgacggcc gtcttcctgg ccacatcact gatcctcgta atagtacgct
      301 gaagccggag gtgaaggcga tgggctcccc gaggctggac aggctctccg acgtatacgc
      361 ctttgagccg gggacggcca ggctcttcag tgcgacccac gaggagatcc tgtccgggaa
      421 gaccaccgat atctacttcg tcaagaccag ggacatcctc gacgcctgcg gaaggctcga
      481 cgctcccgtg gtggccgaga tcttcgccaa gcggggcggt gtcttcgccg gcatccccga
      541 agtggtctcg ctcctggagg acacgggcgt ggagatcctt tccctcaggg aaggggacac
      601 cttcgccccc gacgaggtgg tcgccaggat cagcggcccc tactcggcct tcggcatgta
      661 cgagacggtc atcctgggca tgctggccag ttccaccggg tgggccaccg cggccaggga
      721 atgcgtcgag gccgccgggg gcaggcccgt gctctccttc ggtgcgaggc acgtccaccc
      781 cgccgtctcg tcggtgatgg agagggccgc cgtcgtggcg gggggctgcg tcggcgccag
      841 ctgcatcctg ggagccaaac tggcgggcat agaacccagg ggcaccatcc cccacgccgc
      901 catacttatc gccggggaca ccttgaagat agccaggacc tacgacgact tcctccccga
      961 gggtgaaccc aggatcatcc tggtggacac cttccacgat gaggccgagg aagcgctcag
     1021 gatcgcctcg gccctgggcg agaggctctc ggcggtcagg ctcgacaccc cctccgagag
     1081 gggaggggtc acgcccgaac tggtccgcga ggtccgccgc aggctcgaca tggccggcgc
     1141 ctcccacgtg accatcaccg tgtcgggggg gctcaatccc gagaggataa ggctcctgtc
     1201 ggaggcgggc gccgcctcct tcggcgtggg gagttacatc gcccacgcga cccccaggga
     1261 catgaccatg gacatcaagg tggtcgacgg tgtgcccgtg gcgaaacggg gcaggatccc
     1321 cggcataacc gtcaacccct cccttgaaaa ggcgctctag tgaaaaagcg cccccgcccg
     1381 tccttgacac ccatggggcg cttggataga ataggcaccg ctctgctttt tcgagattca
     1441 cgcagggaag ggaaattatt gtgtccgctg ccttttcgcg gcgaggccat aaggagagga
     1501 acccgggaaa tggctgttaa ttccagttac atggcaaaaa aggaacaggt ggagaggaaa
     1561 tggtacgtca tcgacgccac tgacaaggtc ctcggcaggc tcgccgccca ggtggccatg
     1621 atactccagg gcaagaacaa gccgacctac actccccacg tggacaccgg ggatttcatc
     1681 gtcgtcgtca acgccgacaa ggtaaagatc accggcaaaa aactcgagaa gaagtacctc
     1741 taccgccaca gcagttacaa gggcagcctc aaggcaacgt cctacggcac cttcatggag
     1801 aagtatcccg accgcctctt cgagagggtc gtcaagggga tgctccccaa gaaccgcctg
     1861 cacttcgaga agaaactgaa ggtctacgcg ggacccgaac atccccacgc ggcccagaag
     1921 cctgagccgc tggacatcta gtaacgcggc aggaagggag ggaaaaagag cgtgaagcga
     1981 tctgaatact actggggtac cggcaggcgc aaggccgccc tcgcgagggt gagagtgcgt
     2041 tccggcagcg ggatgctcaa ggtcaacgag cggcccgccg acgagtactt cccccgcagc
     2101 gtctggcagt cccaggcttt tgaggcgctg aggacctcgg ggctcgaggg caagatcgat
     2161 gtcttcgtca acgccagcgg aggcgggctc acgggacagt ccggcgcggt caggctcggg
     2221 ctcgccaggg ccctgctcaa gatggacccc ggtctgaaac ccgctctgaa gagatccggc
     2281 ctgctgacca gggactccag gatggtcgag aggaaaaagt tcggtcagaa aggcgcccgc
     2341 ggcaagaggc agttctccaa gcgctgatct tgcgcggctc ctgc
//
LOCUS       DAOSRG010000190         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_41140_length_2360_cov_5.936659, whole genome shotgun sequence.
ACCESSION   DAOSRG010000190 DAOSRG010000000
VERSION     DAOSRG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41140_length_2360_cov_5.936659"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLM30_05980"
     CDS             complement(<1..674)
                     /locus_tag="PLM30_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HOR54753.1"
                     /translation="MDIRTFLSNEVKPALGCTEPGAVALATSWAAKHGEGPVRSITLR
                     LSPNIFKNGLHAGIPGTEGGTGNLLAAALGALKGDPEKGLTALDGVSAQDVADATDLV
                     NSGVVTQTVVPDVPSVYVEAELADESGTITVIISGRHDRVVQIRKNGRVVKKLLEAES
                     DSGGRLPYIEELLAVDMEGLWELAGGLDDEEVRFLLDGSRMNMEVAEQGLSRSWGLGI
                     GFNLMAL"
     gene            complement(840..1964)
                     /locus_tag="PLM30_05985"
     CDS             complement(840..1964)
                     /locus_tag="PLM30_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR54754.1"
                     /translation="MGLVIKALAEILEQSGFMGLTTGNVIMLVVACILLYLAIGKGFE
                     PLLLVPIATGCLLVNLPLSGIMDQGGFLRYLFYGTEHEIYPVIIFMGIGALTDFSPLL
                     ANPLTFLLGAAAQLGVFVALFGAMMMGFSVPEAASIGIIGGADGPTAIYTTMKLAPQI
                     LGAVAVAAYSYMSLVPLIQPPVIKLLTTRKDRAIRMDVLRPVSKRERVLFPIVSTVAS
                     GLVLPASVPLIGLLMFGNLLRECGVTERLNQAAQNEILNTVTILLGLSVGATMAAESF
                     LTPATIKIIALGLVAFAFSTAGGVLFGQLMKVLSGGKINPMIGAAGVSAVPMAARVVQ
                     RISASENPGNFLLMHAMGPNVAGVIGTAVAAGTFLTLLLN"
     gene            complement(1996..>2360)
                     /locus_tag="PLM30_05990"
     CDS             complement(1996..>2360)
                     /locus_tag="PLM30_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOR54755.1"
                     /translation="AYEVEVEDVGSTASAPAPAPAPAAAPAPAAAPAPAPAPAPAAAP
                     APAAAGDTPVVAPMPGKVLRVLTSVGAAVNTGDVLLVLEAMKMENEIQAPSAGTVKEI
                     KVSDGSPVNTGDVFLVIG"
BASE COUNT          473 a          733 c          742 g          412 t
ORIGIN      
        1 agggccataa ggttgaaacc gatgcccagc ccccaggacc tggagagacc ctgttcggcc
       61 acctccatgt tcatcctgga accgtcgagc aggaaccgga cctcctcatc atccaggccg
      121 ccggcgagtt cccagaggcc ttccatatcc acggcgagaa gttcctcaat atagggaagc
      181 cgtcccccgc tgtcggactc ggcctccaga agttttttca cgacccgtcc gttcttcctt
      241 atctggacga ccctgtcgtg tctgcccgaa atgatcaccg tgatcgtccc gctctcatcg
      301 gccagttcag cctcgacata gaccgaaggg acatcgggga ccaccgtctg ggtaaccact
      361 ccggaattga ccaggtccgt cgcgtcggca acgtcctggg cgctcacccc atcgagagcc
      421 gtcaaacctt tttccgggtc gcccttcaag gcgccgagag cggccgcgag taaatttccc
      481 gtgccgccct ccgtaccagg tataccggca tggaggccgt ttttgaaaat attgggggaa
      541 agccgaagcg taatggacct caccggacct tccccatgct tcgcagccca ggaggtcgcc
      601 agggcaaccg cgcctggctc ggtacagccc aaagccggtt tcacctcgtt cgacaggaac
      661 gtccttatat ccaatggaga aacctccccg aagaaatttt agtcaacgtc acagttttac
      721 accaaagccc cgggggcaac aagtccggcg gtgtatcttc ggtgaagtga aagaaccgcg
      781 gggtccccag ggaccccgcg gtgacggacg cttttttaaa aaaccggtaa aagggagttc
      841 tagttgagaa ggagggtcag gaaggtcccg gccgccacgg ccgttccgat cactccggcg
      901 acgttgggcc ccatggcgtg catgaggagg aagttccccg ggttttcgct ggcgctgatc
      961 ctctggacga ccctggccgc catgggcacg gcggagactc ccgcggcgcc gatcatgggg
     1021 ttgatcttgc cacccgacag gaccttcatc agttgaccga aaaggactcc gccggccgtg
     1081 gagaaggcga aggccaccag gccgagggcg atgatcttga tcgtggcggg ggtgaggaag
     1141 ctctcggcgg ccatggtggc cccgacggaa agccccagca ggatggtcac ggtgttgagg
     1201 atctcgttct gggctgcctg gttcaacctc tcggtgacac cgcactccct gagaaggttg
     1261 ccgaacatca aaagaccgat caggggcacc gaggctggca gtaccagccc cgaggcaacg
     1321 gtgctgacta tggggaagag gaccctctcc ctcttgctga cgggacgcag gacgtccatc
     1381 ctgatggcac gatccttcct ggtggtgagc agcttgatga cgggcggctg gatcagcggc
     1441 accagggaca tgtagctgta ggcggccacc gctacagccc ccaggatctg tggggccagt
     1501 ttcatcgtcg tgtagatggc ggtcggcccg tcagccccac cgataatccc gatggaggcc
     1561 gcctcgggga ccgagaagcc catcatcatg gccccgaaga gtgccacaaa aactccgagc
     1621 tgcgcggcgg cccccaggag gaaggtcagg gggttcgcca ggaggggaga gaagtccgtc
     1681 agggctccta tgcccatgaa gatgatcacg gggtagatct cgtgttccgt tccataaaac
     1741 aggtagcgca ggaaaccgcc ttgatccatg atcccggaga gcggcaggtt gaccagaagg
     1801 caacccgttg ctatggggac caggagaagg ggctcgaacc ccttgccgat ggccaggtaa
     1861 agcaggatac aggccaccac gagcattatc acgtttccgg tggtcaggcc catgaacccc
     1921 gactgttcca ggatctccgc gagggcctta atgacaagtc ccatattgaa cctccccttc
     1981 gaggaccttc aagtcctagc cgatcacgag aaacacgtcg cccgtgttca ccggggaacc
     2041 gtcggacacc ttgatctcct tcacggttcc ggcggaagga gcctggatct cgttctccat
     2101 cttcatcgcc tcgaggacga gcagaacgtc cccggtgttg acggcggcgc cgacgctggt
     2161 gagcaccctc agcaccttac cgggcatggg ggcgaccacg ggggtgtctc cggctgccgc
     2221 cggcgcagga gcggctgccg gggccggggc tggggcggga gcaggggcgg cggcaggtgc
     2281 gggtgccgcg gcgggagcgg gcgctggggc gggagccgat gccgtggaac cgacatcctc
     2341 cacttctacc tcgtaggcga
//
LOCUS       DAOSRG010000191         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_41479_length_2347_cov_8.489092, whole genome shotgun sequence.
ACCESSION   DAOSRG010000191 DAOSRG010000000
VERSION     DAOSRG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41479_length_2347_cov_8.489092"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLM30_05995"
     CDS             complement(<1..970)
                     /locus_tag="PLM30_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR54756.1"
                     /translation="MAEVSETFYPWQIKAWERIRDKDAVLAAPTGSGKTWVAYLWSGL
                     MDLQGRVWPPGTRRVIFTAPIKALSNERYLELRRMGLDVGIETGDFKKNEDAPVICCT
                     QEIYTLKYAGSPGLRVVIDEFHYIFDDPDRARTYIEGIVATSPDSPVLVMSATFGGAR
                     SVTDYLRGITGRPFALYEGRERVTRLVFTPRKPAVLEKLHDALVFCFSQKGSMDLAYK
                     IARRRPRRGKAHRQRIYELAEILDVGDVSMPLLRGVGVYHGGMLPKEKLLVESAFRER
                     ILDVVCGTNALALGVNLPAEYVVFAQLVRFHDREPISRNEFLQMAGR"
     gene            1128..1802
                     /locus_tag="PLM30_06000"
     CDS             1128..1802
                     /locus_tag="PLM30_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR54757.1"
                     /translation="MGSKEVTRKRILEAVGKILAEEGFGGIGVNSVSRRAGVDKVLIY
                     RYFGDLPGLLREFASSGDYWPAMRDLVEEAGRGHRPPRAADLAVALLKGYLRGLRDSP
                     TAQELLLGELVSRNELSEASARKRERQGSDLVEALVRAAPDARGVDVTAMAALLSAGL
                     TFLMLRARTSEAYLGIDISSKEGWERIERAMEELVRGVLGDGGSRLGDEGGAPAAAGV
                     DPVKGP"
     gene            1823..>2347
                     /locus_tag="PLM30_06005"
     CDS             1823..>2347
                     /locus_tag="PLM30_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOR54758.1"
                     /translation="MRRPVLILVIAAVVTVLACGTALGAGFALYDWSARGNALGGAMT
                     GRADDPSAIAFNPAGITQIPGSSMMAGLTFIMPGGTLTNMGTGDSVNMIDHTFIPPHF
                     YYTRQLNDRLWFGVGLLTRFGLGTDFPPDWYGRYNSYYANIQSLSLNPNLAWKVNDRL
                     SLSVGVEALWFEYGA"
BASE COUNT          398 a          717 c          797 g          435 t
ORIGIN      
        1 ccctccccgc catctgcaaa aattcgttcc tggagatggg ttccctgtcg tggaagcgga
       61 ccagctgggc gaagaccacg tactcggccg gcaggttgac gcccagcgcc agggcgttgg
      121 tgccgcagac cacgtcgagg atcctttccc ggaaggccga ctccaccagc agtttttcct
      181 tggggagcat ccccccgtgg tagacgccga cgcccctaag gaggggcatg gagacgtcgc
      241 ccacgtcgag tatctccgcc agttcgtaga tccgctgccg gtgggccttc ccccttcgcg
      301 gccggcggcg ggcgatcttg taggctaggt ccatcgaccc cttctgggag aagcagaaga
      361 ccagcgcgtc gtgcagtttt tccagcaccg ccggtttgcg gggggtgaag accagcctcg
      421 taacgcgctc gcgcccctcg tagagggcga agggcctccc ggtgatgccc cggaggtagt
      481 ccgtcacgga gcgggccccc ccgaaggtgg ccgacatgac cagtaccggg gaatcggggc
      541 tcgtggcgac gataccctcg atgtaggtcc tcgcccggtc ggggtcgtcg aagatgtagt
      601 ggaactcgtc gatcacgacc cgcagccccg gcgagccggc gtacttgagg gtgtagatct
      661 cctgggtgca gcagatgacg ggggcgtcct cgttcttttt gaagtcgccc gtctcgatgc
      721 ccacgtcgag ccccatcctc ctgagttcca ggtagcgctc gttgctcagg gcctttatgg
      781 gggcggtgaa gatgaccctc ctcgtccccg ggggccagac gcggccctgc aggtccatca
      841 gccccgacca caggtaggcc acccaggtct tgcccgaccc ggtgggcgcc gccaggacgg
      901 cgtctttgtc cctgatcctc tcccaggcct ttatctgcca ggggtaaaag gtttccgata
      961 cttcagccat gaccgcctct tttttcgaat tagtgccggg cgaccccggc gctgtctatc
     1021 ttactatgaa cgaccctttc gcgaattcac aattgctttt tccttgacag gggcggaagg
     1081 gggggaatat catcggcttg tgtcacctgc cggtgacagg agggaccatg ggaagcaaag
     1141 aggtcactag gaaaaggatc ctggaagcgg tggggaagat cctcgccgag gaggggttcg
     1201 gcggcatcgg ggtcaatagc gtctcccgga gggccggcgt cgataaggtc ctgatatacc
     1261 gctacttcgg cgacctgccg ggcctcctgc gggagttcgc ctccagcggc gactactggc
     1321 ctgcgatgag ggacctcgtg gaggaggccg gaaggggaca ccgcccgccc cgggccgccg
     1381 acctggccgt ggccctcctg aagggctacc tgcgggggct tcgggattct ccgacggccc
     1441 aggaactgct cctgggggaa ctggtatccc gtaacgagct gtcggaggcc tcggcccgga
     1501 agagggaaag gcagggttct gacctggtcg aagccctggt gagagccgct cccgacgcac
     1561 ggggtgtgga cgtgacggcc atggcggcgc tgctttcggc ggggctgacc ttcctgatgc
     1621 tcagggcccg cacgtcggag gcctacctgg ggatagacat ctcgtccaaa gaggggtggg
     1681 agaggatcga aagggccatg gaggagctcg tgaggggagt cctgggtgac ggcggatcga
     1741 gactggggga tgagggtggc gcacccgcgg cggcgggggt ggatcccgtg aaaggccctt
     1801 gaaaaaaaag gaggagaggt acttgagaag acctgttctt attcttgtta tcgctgcggt
     1861 agtgacggta ctggcttgcg ggacggccct gggtgcgggg ttcgccctct acgactggag
     1921 cgccaggggt aacgcactgg ggggcgccat gaccggccgg gccgacgacc cttcggccat
     1981 agccttcaac ccggcgggaa ttacccagat accgggttcg tccatgatgg cggggctgac
     2041 cttcatcatg ccggggggga cccttaccaa tatgggtacc ggtgacagtg tgaacatgat
     2101 cgaccacacc ttcatccctc cccactttta ctacacccgg cagctgaacg accgcctctg
     2161 gttcggcgtg ggcctgctga cgcgcttcgg cctgggcacc gacttccctc ccgactggta
     2221 tggccggtac aacagctact acgccaacat ccagtccctg tcgctcaacc ccaacctggc
     2281 ctggaaggtg aacgaccggt tgtccctttc ggtgggggtc gaggcgctgt ggttcgagta
     2341 cggggcg
//
LOCUS       DAOSRG010000192         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_41831_length_2333_cov_8.812555, whole genome shotgun sequence.
ACCESSION   DAOSRG010000192 DAOSRG010000000
VERSION     DAOSRG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41831_length_2333_cov_8.812555"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="tyrS"
                     /locus_tag="PLM30_06010"
     CDS             complement(<1..850)
                     /gene="tyrS"
                     /locus_tag="PLM30_06010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOR54759.1"
                     /translation="MQGNPLAIMLERGCVEWCSHPEELSSLFDSEMVTGYIGFDPTAD
                     SLHVGHLIPMMGLAWMQRCGHRPIAIAGMGTGLIGDPSGKSKERNLLTLEQVEKNLVT
                     VKEQLGQFLDFDRGPNSALIVNNHDWLGEQTLLGFLRDTGKYFTVNYMIGREYVKSRL
                     DDPEKSISYTEFSYMLLQAYDFCHLFENFSCKLQMGGNDQQGNIIAGIDLIRKKVGGQ
                     AYGITYPLLLNASGGKFGKTEGGTVWLSAERTSPYRFYQFWINSDDRDVEKLLKLFTF
                     LPLEEIE"
     gene            complement(898..>2333)
                     /locus_tag="PLM30_06015"
     CDS             complement(898..>2333)
                     /locus_tag="PLM30_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54760.1"
                     /translation="FFGRVPVPERVPVPAEAIPSVDPDSPRFYLETLSGQLGPGYPYE
                     TPEKVDDHVRSVILALSDLSPLVDMAERSSVLGAWDANEPALFGAFLLESSQMKELQE
                     GKLPALWLERASGLALGPSESEGILRLTLAKGEVTLFLRADGEFLLVSHSLEGLGKMR
                     KALDGSIERFRPLFNVEPTWPAHIEVFDGKLIAQAASLRGFTAPDKAITLELAWNSRP
                     GSGEAAWKIEGLEEWLPTETREKITPMAWDGPAFLPEPLISAFGVSVPEGFGSLLENE
                     LSIPEWMEEAGFDRASLAGLIEGPLVGVIGGQSRLLLFNLPGVILQLPSRGAEGVRWI
                     RGLWGNKWARFAFSPQPLPGFPAGGKLTLPFTIMAAANEEMAFAGLINEPSLGSMTPV
                     NKIIPMGGERSILWFYADLPKAADALENLSRMAGLVEHFGVDEAPDAQELARLVGQLR
                     SLGQVTLVIHDLGSGRGGWKATVPAAE"
BASE COUNT          479 a          722 c          665 g          467 t
ORIGIN      
        1 cttcgatctc ttcgaggggc aggaaggtga aaagtttgag cagcttctcc acgtccctgt
       61 catcggagtt gatccagaac tggtagaacc gatagggcga tgtcctttcg gcggaaagcc
      121 aaacggtccc gccctcggtc ttcccaaact tgccaccgga ggcattcaag aggagcgggt
      181 aggtgatgcc gtaggcctga ccgcctacct ttttcctgat caggtctatc ccggcgatga
      241 tgttcccctg ctggtcgtta ccgcccatct ggagtttgca gctgaagttc tcgaagaggt
      301 ggcagaaatc gtaggcctgg agcaacatat aggagaactc ggtgtaggag atggactttt
      361 cagggtcatc aaggcggctt ttcacatatt cccgccctat catgtagttc acggtgaagt
      421 atttcccggt atccctcaaa aacccgagca gggtttgctc cccgagccag tcatggttgt
      481 tcacgatgag agcggagttg ggtccacgat cgaaatccag gaactggccc agctgttcct
      541 tgacggtcac caggtttttc tcgacctgtt ccagggtgag gaggttcctc tccttgctct
      601 tgcccgaggg atctccgata aggcccgtcc ccataccggc gatcgcgatg ggcctgtgac
      661 cgcaccgctg catccaggcc agccccatca tcgggatcaa gtgacccaca tgaagactgt
      721 ccgccgtagg gtcaaacccg atatagccgg tgaccatctc gctgtcgaaa agggaggaca
      781 gttcctccgg gtggctgcac cactcgacgc aaccccgctc caacattatg gccaggggat
      841 tcccctgcat ggctacccct ccttgatctg ttccctccgc tgttcccttc gtcaccctca
      901 ctccgctgca gggactgtcg ccttccagcc gcccctcccg gaacccaggt catggatcac
      961 cagggtcacc tgcccaaggg accggagttg accgaccagc ctggccagct cctgcgcgtc
     1021 aggcgcttca tctacaccga aatgctccac caggcctgcc atacgggaaa ggttctccaa
     1081 ggcgtcggcg gccttcggca agtcggcgta aaaccagagg atggatctct ccccacccat
     1141 ggggatgatc ttgttgacag gggtcatact acccagtgaa ggttcgttga tcaggcccgc
     1201 gaaggccatc tcctcattgg cggccgccat gatagtaaag ggcaacgtaa gcttgcctcc
     1261 cgccgggaag cctgggagag gctggggaga gaaggcgaac cgggcccact tgttacccca
     1321 caatccccgg atccaacgga ccccttcggc gcccctcgaa gggagctgga ggatgacccc
     1381 ggggaggttg aaaaggagca gcctgctctg tcctccgatg acgccgacca gaggcccttc
     1441 gatcaggccg gccagggagg cccgatcaaa accggcttcc tccatccatt cggggatcga
     1501 caactcattt tctaacaggc ttccgaatcc ttcgggcacc gaaacgccaa aggcggagat
     1561 aaggggttcc ggtaaaaagg ccgggccgtc ccaggccatg ggggttattt tctcgcgcgt
     1621 ctccgttgga agccattctt ccagcccttc gatcttccac gccgcttcac cgcttcccgg
     1681 ccggctgttc caggccagtt caagcgtgat ggccttgtcc ggggcggtaa accctctcaa
     1741 tgaggccgcc tgggcgatca acttaccgtc aaagacttca atatgggcgg gccaggtcgg
     1801 ttccacgttg aagaggggcc ggaacctctc gatcgaacca tcgagggctt tcctcatttt
     1861 tcccagccct tccagggaat gggagaccag gaggaactcc ccgtcggcac gcaggaagag
     1921 ggttacctcg cccttggcca aggtaaggcg aagtatgcct tcactttcgg agggacccaa
     1981 ggccaggccg gaagctcgct caagccagag agcgggcaat tttccctcct ggagttcctt
     2041 catttgcgag gattccaaga ggaaagcccc aaagagagcg ggttcgttgg catcccatgc
     2101 cccgaggacc gacgacctct ccgccatgtc gactaggggc gacaggtccg acaaggctag
     2161 gatcacggaa cggacatggt catcaacttt ttccggcgtt tcataagggt aacccggccc
     2221 caactgcccc gacagggttt cgaggtaaaa ccgtggggaa tccgggtcca ccgatggaat
     2281 ggcttccgcc ggaacgggga ctctttccgg cacggggacc ctcccgaaga aat
//
LOCUS       DAOSRG010000193         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_42462_length_2307_cov_6.475133, whole genome shotgun sequence.
ACCESSION   DAOSRG010000193 DAOSRG010000000
VERSION     DAOSRG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42462_length_2307_cov_6.475133"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PLM30_06020"
     CDS             <1..1428
                     /locus_tag="PLM30_06020"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54761.1"
                     /translation="GSFGFRGEALASVCAVSRFEIRSRPEGEEGGVLRAEGGVVGLHA
                     SLPCRRGTRVQAEDLFFNLPARRHFLKSAATETRRAVNVVKEYSAAFPRVAFRVLCEE
                     REVFSSGGGDQSRAEALAKVWGTSEAPKKLIVESGPLSLETFWLPAPGSRRRGSIIFF
                     NGRRVRDAAVSAALSSCGEAASGNWMFSITVPAVLLDVNVHPGKAEVRCHSSLAVYDT
                     VRRSVLDLVEGGSLPAESLRAFTAKSPAKWTPAREGGSPFPARQGEVDFFARVGEPPF
                     SPPAEGGRAPGVKKGRFITRLGSGYLLFEETDGLLIIDPHAAHERILFEKLSSAGHPS
                     LAQVVPLQVQLPPSLSHRAEEVKDELESLGFRFKAGSGAMVLESVPGGRHHGGVGDPL
                     DMLRAAVAAIEDGRPPEFTRSGIPGASCRAAVKITDTLAPEEAEALLEDLFRCEEPAL
                     CPHGRPTFIRLLESDLTKLFARSEK"
     gene            1425..>2307
                     /gene="miaA"
                     /locus_tag="PLM30_06025"
     CDS             1425..>2307
                     /gene="miaA"
                     /locus_tag="PLM30_06025"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR54762.1"
                     /translation="MTASPRELFSIIGPTASGKTQLSLELARRLDGEIISVDSRQVYR
                     FMDVGTDKASPGVRREIPHHLIDVADPDETFSAADFAAGASRAVQEILARGRTPILVG
                     GSPFYFKAFFTDVIHDGLERWDDSRGELEKEWDRGGSQELYRRLGELDPEYASRIHPN
                     DRFRIIRALSIIDRTGENPTGLRKGSSGVRKPFRPLYIGLYPGREALYRRIEERVRGQ
                     FDAGYPEEVARLLSMGFGPELPSMKGFGYRELAAFHRGGMTLTEAAEGDIRATKAFAR
                     RQMTWFRRFTPCLWYYVT"
BASE COUNT          391 a          741 c          798 g          377 t
ORIGIN      
        1 ggctccttcg gcttcagggg tgaggccctg gcgagcgtct gcgccgtgag ccggttcgag
       61 atcaggagcc gccccgaggg ggaggagggg ggagtgctac gggccgaagg aggggtcgtc
      121 ggtcttcacg cctccctccc atgccgccgg ggcacccggg tccaggcgga ggaccttttc
      181 ttcaacctcc ccgccaggag gcatttcctg aaaagcgcgg ccaccgagac gaggagggcc
      241 gtcaacgtgg tgaaggaata ctcggcggcc tttccccggg tggccttcag ggtgctctgc
      301 gaggaaaggg aggtcttttc cagcggcggg ggggaccaga gcagggccga agcgctggcg
      361 aaggtatggg ggacgagtga ggcgccgaag aagctcatcg tcgagtcggg gccgctctcc
      421 ctggagacct tctggctgcc ggcgccgggg tcgcggagaa ggggatcgat catcttcttc
      481 aacgggcggc gggtgcggga cgccgccgtt tcggcggccc tctcctcctg cggtgaggcg
      541 gcctcgggga actggatgtt ctccatcacc gtccccgctg tgctgctcga cgtgaacgtg
      601 caccccggca aggccgaggt gcgatgccat tcctccctgg ccgtctacga cacggtgagg
      661 agatccgtcc tcgatctggt ggagggaggg agcctccccg ccgagagcct gcgggccttc
      721 acggcgaaga gcccggcgaa atggaccccc gcgagggaag gcggaagccc tttccctgcc
      781 aggcaagggg aagtggactt tttcgccagg gtgggggaac cgcctttttc gccccccgcc
      841 gagggaggcc gtgcccccgg ggtcaagaaa ggccggttca taaccaggct cggctcgggc
      901 tacctgctct tcgaggagac cgacgggctc ctgataatag acccccacgc cgcccatgag
      961 aggatcctct tcgagaaact gtcctcggcg ggccatccct ccctcgccca ggtggtcccc
     1021 ctgcaggtcc agctgcctcc ttccctgtcg caccgggcag aggaggtcaa ggacgaactg
     1081 gagagcctgg gcttcaggtt caaagcgggg tcgggggcca tggtcctcga gtccgtcccc
     1141 ggcggccgtc atcacggagg ggtgggtgac ccgctggaca tgctcagagc cgccgtggcc
     1201 gccatcgaag acggccggcc gccggagttc acccggagcg gcatacccgg ggcctcctgc
     1261 agggcggcgg tcaagataac cgacaccctg gcccccgagg aggccgaggc gctgctggag
     1321 gacctcttcc gctgcgagga accggccctc tgtccccacg gcaggcccac ttttatcagg
     1381 ctgcttgaaa gcgacctgac gaagctcttc gccaggagcg aaaaatgacc gcctcccccc
     1441 gggaactatt ctccatcata ggtcccactg cttcggggaa gacccagctc tcccttgaac
     1501 tggcccgaag gctcgacggc gagatcatct cagtggactc ccgccaggtc taccgcttca
     1561 tggatgtcgg gaccgacaag gcctcccccg gggtcaggag agagatcccc catcacctca
     1621 tcgacgtggc ggaccccgat gagaccttct ccgcggcgga cttcgccgcc ggcgcgtcga
     1681 gggccgttca ggagattttg gccaggggaa ggacgcccat cctcgtgggc ggctcaccct
     1741 tttacttcaa ggcctttttc accgacgtca tccacgatgg cctcgagcga tgggacgaca
     1801 gcaggggcga gttggagaag gaatgggacc ggggagggtc ccaagagctc taccggaggc
     1861 tgggggagct ggaccccgag tacgcctccc ggatccaccc caacgaccgt ttcaggatca
     1921 tcagggccct ctccatcatc gacaggacgg gcgaaaatcc cacgggcctc aggaaggggt
     1981 cgtcgggggt ccgcaaaccc ttcaggccgc tgtacatcgg gctttacccg gggagggagg
     2041 ccctttaccg gaggatcgag gagagggtgc gggggcagtt cgacgccggc taccccgagg
     2101 aggtggcccg cctcctttca atgggtttcg ggcccgaact tccctccatg aagggcttcg
     2161 ggtacaggga gctggcggct tttcatcggg gcgggatgac cctgaccgag gcggccgagg
     2221 gggacatcag ggccaccaag gccttcgccc gaaggcagat gacctggttc aggaggttta
     2281 ccccgtgcct gtggtattat gtcaccg
//
LOCUS       DAOSRG010000194         2295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_42753_length_2295_cov_12.466518, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000194 DAOSRG010000000
VERSION     DAOSRG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42753_length_2295_cov_12.466518"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="tuf"
                     /locus_tag="PLM30_06030"
     CDS             complement(<1..1066)
                     /gene="tuf"
                     /locus_tag="PLM30_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR54763.1"
                     /translation="MAKEHFDRTKPHLNIGTIGHIDHGKTTLTAAITRTLSSTGWADF
                     TPFDKIDKAPEERERGITINISHVEYQTEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVNVPALVVFMNKVDMVDDPELLDLVEMEIRDLLGK
                     YDFPGDDTPIIRGSALKALESDDLGRDSQWAGAIWELMDACDSFIPEPVREVDRPFLM
                     PIEDVFTITGRGTVVTGRVERGIIKAGDEVSIIGMQEDIKKTVATSLEMFRKILDDAR
                     AGDNVGVLLRGVGKEDVERGQVLAKPGSITPHKHFKSEVYVLKKEEGGRHTPFFSGYK
                     PQFYFRTTDVTGDIKLPEGVE"
     gene            complement(1132..>2295)
                     /locus_tag="PLM30_06035"
     CDS             complement(1132..>2295)
                     /locus_tag="PLM30_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR54764.1"
                     /translation="PDPGGPFSALAFKIMVDPFVGRLVYCRIYSGRVRSGSSLLNTST
                     GNRERVGRILRMHANKREDLEEASAGEIVALPGLKATKTGDTLSDERKPIVLESVEFP
                     EPVVHLSAEPMSKADQVKLTRGLAGLSEEDPTFKVSTNEETAQTIISGMGELHLEIIV
                     DRLKREYGVDVRVGRPQVAYRETIRNPSSGEGKFIKQTGGRGQYGHVVLEIEPLPEEK
                     GFLFEDRIVGGAIPREFIPAVQKGIAETVANGVLGGYPVIGVKVTLVDGSFHEVDSSE
                     LAFRIAASIAFKEAMRSANPVLMEPIMEVEVVTPDEYVGDVMGDLSSRRGHVEGMEMR
                     GNARIVKSFAPLSEMFGYATDLRNKTSGRASYSMQFSHYEEVPGEITEKILRH"
BASE COUNT          371 a          778 c          654 g          492 t
ORIGIN      
        1 tctcaacgcc ctcgggcagc ttgatgtctc ccgtcacgtc ggtcgtcctg aagtaaaact
       61 gcggcttgta cccgctgaag aacggcgtgt gacggccgcc ctcttccttc ttcagtacgt
      121 acacctcgga cttgaagtgc ttgtggggcg tgatgctccc cggcttcgcc aggacctgcc
      181 cccgctccac gtcctccttg cctacgccgc gaaggagcac tcccacgttg tcacccgccc
      241 gggcgtcgtc gaggatcttc cggaacatct caagcgatgt cgccaccgtc ttcttgatgt
      301 cctcctgcat tccaataatg gacacttcgt cgccggcctt gatgatcccc cgctccaccc
      361 tgcccgttac aacagtgccg cggcccgtta tggtgaagac gtcctcgatg ggcatcagga
      421 agggcctgtc cacttcacgc accggctcgg ggatgaagct gtcgcaggcg tccatcagtt
      481 cccagatcgc accggcccac tggctgtcgc ggccaaggtc gtcgctctca agggccttca
      541 gagccgaacc acggatgatg ggcgtgtcgt cgccggggaa gtcgtacttg cccaggaggt
      601 cacggatctc catctccaca aggtccagca gttcagggtc gtccaccatg tccaccttgt
      661 tcatgaagac caccagcgcc ggcacgttga cctgccgggc caacagtacg tgctcacgcg
      721 tctggggcat ggggccgtcc gccgcggaga ccaccaggat cgctccgtcc atctgcgccg
      781 caccggtgat catgttcttg atgtagtcgg cgtgaccggg gcagtcgatg tgggcgtagt
      841 gacgcttctc cgtctggtat tccacgtggg agatgttgat cgtgatgcca cgctcgcgct
      901 cctcgggggc cttgtcgatc ttgtcaaagg gcgtgaagtc cgcccacccc gtcgaggaca
      961 gcgtgcgcgt tatcgccgcc gtcagcgtcg tcttgccgtg gtcaatgtga ccgatcgtac
     1021 ctatgttcag gtgcggcttc gtcctgtcaa aatgctcctt cgccatctcg taaacctccc
     1081 ttgttaactt cttctcagcg gttaccgctt tttatgtttt tccggggcgt atcagtgccg
     1141 caggatcttt tcggtgatct ccccgggaac ttcctcgtag tgagagaact gcatcgagta
     1201 ggacgccctg cccgatgtct tgttcctcag gtcggtggcg tatccgaaca tctccgagag
     1261 cggggcgaag gacttcacga tcctcgcgtt gcccctcatc tccatcccct ccacgtgacc
     1321 ccgccgcgag gaaagatccc ccatcacatc gcccacgtat tcatcggggg tcacgacctc
     1381 gacctccatg atcggctcca tcaggaccgg gttggcggac ctcatggctt ccttgaaggc
     1441 aatggaggcg gcgatccgga aggccagttc ggagctgtcc acctcgtgga agctgccgtc
     1501 gacgagggtc accttcacac cgatcacagg gtaaccgccc agcaccccgt tggccacggt
     1561 ctcggctatc cccttctgca cggccgggat gaactccctc ggtatggccc cgccgacgat
     1621 cctgtcctcg aaaaggaagc ccttttcctc ggggagcggc tcgatctcca ggaccacgtg
     1681 gccgtactgc cccctgcccc cggtctgctt gatgaacttg ccctcgccgg aggaggggtt
     1741 cctgatggtc tcccggtagg ccacctgggg ccttcccacc ctgacgtcca cgccgtattc
     1801 cctcttgagc cggtcgacga tgatctcgag gtgcagttcc cccatcccgg agatgatcgt
     1861 ctgggccgtc tcctcgttgg tggagacctt gaaggtggga tcctcctcgg agagccccgc
     1921 cagaccccgg gtcaacttca cctggtcggc cttgctcatg ggttccgccg agaggtggac
     1981 caccggctcc gggaactcca ccgattccag gacgatgggc ttcctctcgt cggagagggt
     2041 gtcccccgtc ttggtggcct tcagccccgg cagggcaacg atctcgccgg cggaagcctc
     2101 ttccaggtct tccctcttgt tggcgtgcat cctgagtatc ctgcccactc tctcgcggtt
     2161 ccccgtagaa gtattcaaaa ggctggaacc gctcctgacc ctccccgaat agatcctgca
     2221 atagaccagg cgccccacga aggggtcgac cattatcttg aaggccagcg ccgagaaggg
     2281 accgccgggg tcggg
//
LOCUS       DAOSRG010000195         2269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_43421_length_2269_cov_7.647696, whole genome shotgun sequence.
ACCESSION   DAOSRG010000195 DAOSRG010000000
VERSION     DAOSRG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43421_length_2269_cov_7.647696"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLM30_06040"
     CDS             <1..722
                     /locus_tag="PLM30_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR54765.1"
                     /translation="IYVDEIDKIGRKSESVSITRDVSGEGVQQALLRILEGTEANVPP
                     KGGRKHPHQEFIQMKTDNILFICGGAFEGLESIISRRVNRKIIGFGGQVTRNDKKDRY
                     RILKEVQPDDLMTFGFIPEFVGRIPVTAALEGLDEEAMVRILTEPKNALVKQYQKAFR
                     MEKVELEFTSEALRAIARRACERDTGARALRTIMESTMLDLMYELPSRTNGLSRVVVT
                     QEMIDGKGLPIFDFTKDGEVA"
     gene            723..>2269
                     /gene="lon"
                     /locus_tag="PLM30_06045"
     CDS             723..>2269
                     /gene="lon"
                     /locus_tag="PLM30_06045"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOR54766.1"
                     /translation="MPYLGEGPDKKVPLLPVRDLVLFPGVIVPLFVGRPRSLKALEEA
                     MLHDKTVLVVAQKDMNVEDPEPEDLFEVGTITTVLQMLRLPDGTTKVLVEGVSRASIL
                     EVGLERDTLFAKTEALETPPPLRGPEIEALKRTLLAQFDKYVTLHPKIPLEVLMSVTA
                     VEDPGQVSDLVASHLTVRIQERQQLLETWDVSKRLEMMIRILLREIELLEMERSIHDR
                     VRQELEKGQKEYYLREQLKVIQDELGQGDEFSEIDNLREEIHRSKMPEEVRKKALHEL
                     DRLTKMPSMSAEATVVRTYLDWMTNLPWGKRTRERMDVTLAERVLNEDHYALEEAKER
                     ILEYLAVRKHAKGRLKGQVLCFAGPPGVGKTSLGRSIARATGRKFVNMSLGGIRDEAE
                     IRGHRRTYIGALPGRIIQKLRQAGTCNPVMLLDEVDKIGQDFRGDPAAALLEVLDPEQ
                     NDSFTDHFLEVPFDLGNVLFITTANTTHTVPKPLLDRMEVIRLPGYVTEEKLQIAEKH
                     LIPKVLY"
BASE COUNT          542 a          604 c          720 g          403 t
ORIGIN      
        1 tcatctatgt cgacgagata gacaagatcg gcaggaaatc cgaatcggtc tccataacga
       61 gagacgtctc gggggaaggt gtccagcagg cactgttgag aatactcgag ggcaccgagg
      121 cgaacgttcc ccccaagggc ggcagaaagc accctcacca ggagttcatc cagatgaaaa
      181 ccgataacat cctgttcatt tgcggggggg cctttgaagg gctggagtcc atcatttcac
      241 ggagggtcaa caggaagatc atcggttttg ggggccaggt caccaggaac gataagaaag
      301 accgttacag gatccttaag gaagtccagc ccgacgacct gatgaccttc gggttcatcc
      361 ccgaattcgt tgggaggata ccggtaacag cggccctgga gggtctggat gaagaggcga
      421 tggtcagaat actgacggag ccgaagaatg ccctggtcaa gcagtaccag aaggctttca
      481 ggatggagaa ggtggaactg gaattcactt ccgaagccct aagggccatc gccaggaggg
      541 cctgtgaacg tgataccggc gctcgggccc tgaggaccat catggagtcg acaatgctgg
      601 acctgatgta cgaactccct tcgcggacaa acggcctttc cagggtggtc gtcacccagg
      661 agatgatcga cgggaagggg cttccgatct tcgattttac caaggacggg gaggtagcct
      721 agttgcctta cttaggagag ggtcccgata agaaggtgcc gctgctcccc gtcagggacc
      781 tggtcctttt cccgggggtt atagtgcccc tctttgttgg gaggccccgg tcgctcaagg
      841 cccttgagga ggccatgctc cacgataaga cagtcctcgt ggtggcccag aaggatatga
      901 acgtggaaga cccggaaccg gaggatcttt tcgaagtagg gaccattacc acggtcttgc
      961 agatgttgag gctccccgac gggacaacaa aggtcctggt cgagggggtg tcaagagctt
     1021 ccatcctcga ggtcgggttg gagcgggaca ccctttttgc gaagaccgaa gccctggaga
     1081 cacccccacc cctgaggggg ccggagatcg aagctctgaa aaggaccctc ctggcgcagt
     1141 tcgacaagta tgtgaccctt caccccaaga tcccgctgga ggtgttgatg tcggtaacgg
     1201 ccgtggaaga ccccggccag gtttcggacc tggtggcatc ccaccttacg gtccgcatcc
     1261 aggaaaggca acagctcctc gaaacctggg acgtatcgaa acgcctggag atgatgatca
     1321 ggatcctcct gagggagatc gagctgctcg agatggagag gtccatccac gacagggtga
     1381 ggcaggagct cgaaaagggt cagaaggaat attacctgag agaacagttg aaggtcatcc
     1441 aggatgaact gggtcagggc gacgagttct cggagatcga caacctccgt gaggagatcc
     1501 accggtcgaa gatgcccgag gaggtcagga aaaaggccct ccacgaactg gaccggctga
     1561 ccaagatgcc ctccatgtcg gcggaagcca cggtggtgag gacctatctc gactggatga
     1621 cgaacctccc ctgggggaag aggacccggg aaaggatgga cgtgaccctg gccgaaaggg
     1681 tcctgaacga ggaccattat gccctcgaag aagcaaagga gaggatcctg gagtaccttg
     1741 ccgtaaggaa acatgccaag gggaggttga aagggcaggt cctctgtttc gccggccccc
     1801 ccggcgtcgg taagacctcc ttggggcgct ccatcgccag ggcgacggga agaaagttcg
     1861 tgaacatgtc cctgggaggg atcagggacg aggctgagat ccgcggacac cggaggacct
     1921 atatcggggc tttgcccggg aggataatcc agaagctaag acaggcgggg acgtgcaacc
     1981 cggtgatgct cctggatgag gtcgacaaga tcggccagga tttccgcggt gaccccgccg
     2041 cggcgcttct ggaggtgctt gacccggagc agaacgacag cttcaccgac catttcctgg
     2101 aagtaccctt cgacctgggg aatgtccttt tcatcacaac ggcgaatacg acccacaccg
     2161 tccccaagcc gttactggac cggatggagg tcatccggtt accgggttac gtgacggagg
     2221 agaaactgca gatcgccgag aaacacctca ttcccaaggt cctctacga
//
LOCUS       DAOSRG010000196         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_43444_length_2268_cov_8.815635, whole genome shotgun sequence.
ACCESSION   DAOSRG010000196 DAOSRG010000000
VERSION     DAOSRG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43444_length_2268_cov_8.815635"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLM30_06050"
     CDS             complement(<1..234)
                     /locus_tag="PLM30_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54767.1"
                     /translation="MDDLERRVVILSLVARKGDNPDLWGYARLRKSILLLQVEEDVPL
                     GFVFGMERERPVSPELQLFLERMLYDGYLLSVET"
     gene            457..1614
                     /locus_tag="PLM30_06055"
     CDS             457..1614
                     /locus_tag="PLM30_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOR54768.1"
                     /translation="MKAVQIGGGLVGQVIAADMMKEFEVTVVDLDVDTLGEVERKCPG
                     VRTALAPATDPDRLAPLLGDADIVTAGVPGRLGFEMMKTVINLGKNLVDISFMAEDFE
                     ELDGLAREKGVTVIPDMGVAPGMSNFLMGRGASLLDEVEDAFIFVGGIPAKAFPPFNY
                     QVTWSPKDCIEEFTRPVTIVKDFRQEVVEATSGLHLRDVPGVGTLEAFYTDGLRSLAR
                     NIRARNLGEMTLRWPGHVEQMRLLRSMGLFEETPRTLGGKEVVPLEVVADLLFPMWKM
                     EPEKGDRDLTVMEVDVHGFRGLDEMTHSWRLLDHFDEKTWNTSMSRCTGCSCAIFARA
                     VAHGFVKQKGVLPAEKLAHDYALYNFVMEEQRKRRIKYTESIKKVLNVRRP"
     gene            1904..>2268
                     /locus_tag="PLM30_06060"
     CDS             1904..>2268
                     /locus_tag="PLM30_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOR54769.1"
                     /translation="MSEKQQVKPSLGLSVGVFVAAAVIISYGVLGLEVDAHVPIVFSA
                     VLVCAIGLTVLKMPWSQIEEGALNAISIALQAIVILIIIGMIIGIWIQSGVVPTLIYY
                     GLSILSPSIFLLATLLICSV"
BASE COUNT          468 a          634 c          671 g          495 t
ORIGIN      
        1 ggtctccacg gataacaggt aaccgtcgta gagcatcctt tccaggaaga gctgtaattc
       61 gggggaaacc ggcctttcgc gctccatgcc gaagacgaaa cccaggggta cgtcctcctc
      121 gacctggagg agcaggatcg atttgcggag cctcgcgtaa ccccacaggt ccgggttgtc
      181 gcccttcctg gccaccaggc tgaggatgac aacacgcctt tccaggtcgt ccatcaaagt
      241 accccccgcg tcgtgtacac ttggagctaa catactttac caaagcgttg agaaaaaaca
      301 agggcgcact acggcccctg acgggaaggc tgcccctttt ttaagctccc cgccggggcg
      361 gtccaccttt gattgactct cttttcattg ccatctatac taatttatgc atacaataat
      421 gtgaaaggga cggccatttt taaaggagtg atcgggatga aggcggtgca gatcggcggg
      481 ggcctcgtgg gccaggtaat agcggcggat atgatgaagg agttcgaggt gaccgttgtg
      541 gacctcgacg tcgacaccct cggcgaagtc gagaggaaat gccccggagt caggacagcc
      601 ttggcaccgg ccacagaccc ggatcgcctc gcccccctgc tcggggacgc cgatatcgtg
      661 acggcggggg tcccgggccg tttaggcttc gagatgatga agaccgtcat caacctgggg
      721 aagaacctgg tcgacatctc cttcatggcg gaggatttcg aggaactgga cgggctcgcc
      781 agggaaaagg gcgtgaccgt catacccgac atgggcgtcg ctcccgggat gtccaacttc
      841 ctcatgggga ggggggcttc gctgctcgac gaggtggagg atgcctttat cttcgtcggg
      901 ggcatccccg ccaaggcctt tccccctttt aactaccagg tcacctggtc ccccaaggac
      961 tgcatcgagg agttcacccg ccccgtgacc atcgtgaagg acttccgcca ggaagtggtt
     1021 gaagccacct cggggctcca cctccgcgac gtcccgggcg tggggaccct cgaggccttt
     1081 tacaccgacg gcctgaggag cctggccagg aacatcaggg ccaggaacct cggcgaaatg
     1141 accctgagat ggcccggtca cgtggagcag atgaggctcc ttcggtccat gggccttttc
     1201 gaggagaccc ccaggaccct cgggggcaag gaggtcgtcc ccctggaggt cgtcgcggac
     1261 ctgctctttc ccatgtggaa gatggagccc gagaagggcg acagggacct caccgtcatg
     1321 gaagtggacg tgcacggttt caggggtctc gatgaaatga cccactcctg gcggctgctg
     1381 gaccacttcg atgaaaagac ctggaatacc tccatgagcc gctgcactgg gtgctcctgc
     1441 gccatcttcg ccagggccgt cgctcatggg ttcgtgaagc agaagggcgt cctgccggcg
     1501 gagaaactgg cccatgacta cgccctttat aacttcgtga tggaggaaca gaggaagaga
     1561 aggataaaat acactgaatc aataaagaag gtgttgaatg taaggcggcc ttgagggccc
     1621 tatcatcgct ggggccgctg attaaaataa aaaatcatgt ataatgacct tgtaccgatc
     1681 ggaagcctgt gatactttcc ataagtgaac cgtccctcag gcggggggca ggatcccata
     1741 attacgggtt taccgatgcc gcccacgggc gttttaaggc aggtcacggc gcgcccgcgt
     1801 aacggtctcc ctggagagga ggtggggctg ggcgttattc ctttcggttc cttaaggtct
     1861 ccatggatct gagttttaat cctttgagaa ggaggttttt tttatgtcgg agaaacagca
     1921 agtaaagccc agtctgggcc tttccgtcgg cgtgtttgta gccgccgccg taatcatcag
     1981 ttatggtgtt ctgggattgg aagtggatgc ccacgttccc atcgtttttt ccgccgttct
     2041 tgtttgcgcc atcggtctga cggtcctgaa gatgccttgg tcccagatcg aggaaggtgc
     2101 ccttaacgct atttctattg ctctccaggc gatcgtcatc ctcataatca taggtatgat
     2161 catcggcata tggattcaaa gcggagttgt gcccaccctg atctactacg gcttgtccat
     2221 cctttcgccg tcgatcttcc tgctggccac cctactgatc tgttccgt
//
LOCUS       DAOSRG010000197         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_43470_length_2267_cov_9.600362, whole genome shotgun sequence.
ACCESSION   DAOSRG010000197 DAOSRG010000000
VERSION     DAOSRG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43470_length_2267_cov_9.600362"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..1190)
                     /locus_tag="PLM30_06065"
     CDS             complement(333..1190)
                     /locus_tag="PLM30_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR54770.1"
                     /translation="MTRPLRLEFPGAVYHVMSRGDNKQKIFLNDHDRRRFLKIYQDVV
                     ERQSWMTYAWCLMDNHFHLVIETPEPNLSEGMRLLNGIYTQYFNANKGRVGHVFQGRF
                     HSIVVDENVYLLELVRYVVLNPVRSGRVDNPGKWPWSSYKATAGLEPCPPWLNADEVT
                     DAVSERIGTRRDRHKSYADFILKGMGRDEDLMEKVQQQVYLGDAEFIVKVQDRCSVSP
                     ELADIAREQKRKPLASLEEYFGQYPHPKEAMAMAYLEGRFNQAEISRHLGVHYSTVSR
                     AVREYKKKA"
     gene            1382..>2267
                     /locus_tag="PLM30_06070"
     CDS             1382..>2267
                     /locus_tag="PLM30_06070"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54771.1"
                     /translation="MNVLGREEIFELAEHESTPSVSLYMSGTRPHDPKKTQIRFKNLV
                     ARAEEILGGFDLRPHQKEALLTDPRSLLSNSVFWERQDESLALFASAGVFRVLNVPSE
                     VSDVVTVSDRFYIKPLLPLLAGEEEYLVLCLSQKQIRLLRFGRGAVGEVPLEGIPTSL
                     SEALQYNPMEKQGQFGGAYPTHGAEEEDFKANIRRFFRIVDDGLQKILREEKLPLVTA
                     GVSSLNSIYREANTYAGLLEGAVEGNPEAFKDSEIREKAWKIVKPLFERSKKEALERF
                     GHLQGTGKTSVSLEEVVPA"
BASE COUNT          447 a          697 c          611 g          512 t
ORIGIN      
        1 gcgatctggt attcgctgga gagtttctcg tattcctccc tgaattggga gcttttaaga
       61 agttcttttt tgatcttttt gaattcagtg atcattgttt atcctccttg ctctttgccg
      121 ggcaacttcg atgtcagttc ttgtcagtcg ggcggttttc ttgtgtatca ctctcaatat
      181 gtaaatgttt ctccccgttc tggtcacata tattcctctt gcgatcccgc tttttgcctt
      241 tgctctcaat tcccagagat cattctcaat atgtttgaca aagggcatat ccctgattgg
      301 ggtcagacct tgaatttgag catttgtata ctttaggcct tcttcttgta ctcccttacg
      361 gccctgctca cggtgctgta gtggacaccc aggtgccgtg atatctccgc ctggttgaac
      421 ctcccctcca ggtaggccat ggccatggcc tccttcgggt gggggtactg tccgaagtat
      481 tcctcgaggc tggccagggg ctttctcttt tgctccctgg cgatatcggc gagttcgggg
      541 ctcacggagc acctgtcttg caccttcacg atgaactccg cgtcgccaag atagacctgc
      601 tgctggactt tctccatcag gtcttcgtcc ctgcccatgc ccttcaggat gaagtcggca
      661 tagctcttat ggcgatcccg ccttgtgccg atcctttcgg agaccgcgtc ggtcacctcg
      721 tccgcattca gccagggcgg gcagggctct agcccggccg tcgccttgta actgctccac
      781 ggccatttcc ctgggttatc gacccggccg gaccgtacgg ggttcaaaac cacgtacctc
      841 accagttcca ggaggtagac gttctcatcg accactatcg aatggaacct cccctggaaa
      901 acatgcccga ccctgccctt gttcgcgttg aaatactggg tgtagatgcc gttgaggagc
      961 ctcatgccct cggaaaggtt cggttcgggg gtctcaatga ccaggtggaa gtgattgtcc
     1021 atcaggcacc aggcgtaggt catccacgac tggcgctcca cgacgtcctg gtagatcttc
     1081 aggaaccgcc gccggtcgtg gtcgttgagg aatatcttct gcttgttgtc cccccgggac
     1141 attacgtggt agacggcgcc ggggaactct agccttaagg gtctggtcat gataaagacg
     1201 atagcataca aatgatcaaa ttcaaggtct gaccccaaga ggggcttcat gctataattt
     1261 acccgagtga agtgccacga gtgctgagca ccgtccctgc ggcaagccgg ggcgccttaa
     1321 aaacagaaca gatgcgttcc gcccgccggg atgaaacccc actacaagga ggcggtgaac
     1381 cgtgaacgta ctgggccgag aggaaatatt cgaactggcg gaacacgaga gcacgcccag
     1441 cgtctctctc tacatgtcag ggacccgtcc gcacgacccc aagaaaacgc agatccgctt
     1501 caagaatctc gtcgccaggg ccgaggagat cctcggcggg ttcgacctgc gcccccacca
     1561 gaaggaggcc ctcctcaccg accccaggtc gctgctttcc aactccgtct tctgggaacg
     1621 ccaggacgag agcctcgccc tcttcgcctc cgccggcgtc ttccgggtcc tcaacgtccc
     1681 ctccgaagtg agcgacgtgg tgaccgtgtc ggaccgcttc tacataaaac ccctgttgcc
     1741 cctcctggcg ggggaagagg agtaccttgt cctttgcctg agtcagaagc agatccgcct
     1801 gctccggttc ggtcggggcg ccgtcggcga ggtgcccctc gagggaatcc ccacgagcct
     1861 ctccgaggcc cttcagtaca accccatgga aaaacagggc cagttcggcg gggcctatcc
     1921 cacccacggc gccgaagagg aggacttcaa ggccaacatc cggaggttct tccgaatcgt
     1981 cgatgacggc ctccagaaaa tactccgcga ggaaaaactg ccccttgtca cggcgggcgt
     2041 aagctccctc aactcgatct accgcgaggc taacacctac gccggcctcc tggaaggagc
     2101 cgtcgagggg aaccccgagg ccttcaaaga cagtgagatc cgggagaagg cctggaagat
     2161 cgtcaaaccc ctcttcgagc gatcaaagaa ggaagccttg gaacgcttcg gacacctcca
     2221 ggggacgggc aagacctccg tctccctcga ggaagtcgtc cccgcct
//
LOCUS       DAOSRG010000198         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_43764_length_2255_cov_7.741818, whole genome shotgun sequence.
ACCESSION   DAOSRG010000198 DAOSRG010000000
VERSION     DAOSRG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43764_length_2255_cov_7.741818"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLM30_06075"
     CDS             complement(<1..287)
                     /locus_tag="PLM30_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54772.1"
                     /translation="MRRRNNTRLEPTSPVSLYVHIPFCRSKCPYCAFYSVIPTPGEVT
                     AFLEALEGEIGFLSKSSDRPLLRTAYIGGGTPTLLSPRDWERLITVLESAFS"
     gene            complement(271..2088)
                     /gene="lepA"
                     /locus_tag="PLM30_06080"
     CDS             complement(271..2088)
                     /gene="lepA"
                     /locus_tag="PLM30_06080"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOR54773.1"
                     /translation="MKVPIERIRNFCIIAHVDHGKSTLADRLLEATTTVSAREMREQI
                     LDTLELERERGITIKLVPVQMKYRASDGLDYVLNLIDTPGHVDFTYEVSRSLAACEGA
                     LLIVDAAQGVEAQTVANAYLAVDQGLEIIPVINKIDLPSAQPEQAAKEIEEVVGLDSS
                     EAILASAKEGIGVKEILEAVIKKIPPPFKDRLGPLQALVFDSTYNNFRGVICYIKVAN
                     GTIRTGDMVTFMATRRSFQVEEIGIFGPEMRPVDELGPGEVGYLICNIKSVEEARVGD
                     TVTSQRNPASTPLPGYRKVKPVVFCGFFPIERDEYPQLRDSLEKLQMNDAALFFEPET
                     STALGFGFRCGFLGLLHMEIAKERLNREFGVDLVATVPNVVYQVKITSGEIIEAHRPS
                     DFPEAPLIEEVLEPFIKVTGFVPAEYVGKVMQLCQDRRGVFLGMDYITPQRTRLVYEL
                     PLAEFIIDFHDRLKSVTRGFASIDYEHSGFKVSNLVKVDILIGGEPVDAFCFVSHADQ
                     AYHRGQDVVRKLKEIIPRQLFEVSLQAAIGKRVIVRQNVKPLRKDVLAKCYGGDITRK
                     RKLLEKQKEGKKRMKQIGRISLPQEAFLAFLKSSDEEEE"
BASE COUNT          488 a          628 c          638 g          501 t
ORIGIN      
        1 gaaaaggcac tttcgaggac agttataagt ctttcccagt cgcgagggga aaggagcgta
       61 ggagtcccac cgcctatgta ggccgtcctt aaaagcggac gatctgaact tttcgagaga
      121 aagcctatct caccttcaag agcctcaaga aaggccgtca cttcgcccgg cgtggggata
      181 acgctgtaaa aagcacagta ggggcatttg ctccggcaga aaggaatgtg aacgtaaaga
      241 gaaaccggcg atgtgggctc gaggcgcgtg ttattcctcc tcctcatcgc tggacttgag
      301 aaaggcgagg aaagcctcct ggggcaagga tatcctgccg atctgtttca tcctcttttt
      361 cccctccttc tgcttttcga gcaatttcct cttcctggtg atatcaccgc catagcactt
      421 ggcgaggaca tccttgcgta aaggcttgac gttctggcgg acgatcaccc ttttgcctat
      481 cgcggcctgg agggagacct cgaagagctg cctcgggatg atctccttga gcttccggac
      541 cacatcctgg ccgcggtgat acgcctggtc agcgtgggat acgaagcaga aagcatcgac
      601 gggctcaccg ccgatcagga tatcaacctt gacaaggttg gagaccttga aaccggaatg
      661 ctcgtagtct atcgaggcga atcccctcgt cactgacttg agcctgtcat ggaagtctat
      721 tatgaattcc gccagaggga gctcatagac caggcgggtt ctctgggggg tgatgtagtc
      781 catccccagg aagacgcccc tgcggtcctg gcagagctgc atcaccttgc ccacgtattc
      841 cgccgggacg aagccggtga cctttatgaa gggttcgagg acctcctcga tgagaggggc
      901 ttcgggaaag tccgaaggac ggtgcgcttc aatgatctct ccgctcgtga tcttgacctg
      961 gtataccacg ttgggtaccg tggcgaccaa gtccacaccg aactccctgt tcagccgttc
     1021 cttcgcgatc tccatgtgca aaagcccaag gaacccgcac cggaagccaa atccaagggc
     1081 cgtcgaagtc tccggttcga aaaacagggc cgcatcgttc atctgcaatt tttcgaggga
     1141 atctctcagc tggggatact catccctttc tatggggaaa aaaccgcaga agactacggg
     1201 cttgaccttc cgataaccag gtaaaggtgt agaagccggg ttcctctggc tagttaccgt
     1261 atcgccaacc ctggcctctt ccacggactt gatattgcag ataaggtagc cgacctcccc
     1321 gggccccagc tcgtcgacgg ggcgcatctc gggcccgaag atccctatct cctccacctg
     1381 gaaacttctc ctggtcgcca tgaaggtcac catgtcgccg gtcctgatgg tgccgttggc
     1441 cacctttatg tagcagatga cccctctgaa gttgttgtag gtggagtcga aaacaagggc
     1501 ctggaggggg cccaagcgat ctttaaaggg aggggggatc ttcttgatca ccgcttccag
     1561 gatctccttt accccgatac cctccttggc gctggccagg atcgcctctg agctgtcgag
     1621 tccgaccact tcctcgattt ctttcgctgc ctgctcgggc tgggccgagg ggaggtcgat
     1681 cttatttatt acaggaatga tctcgaggcc ctgatcaacg gcaagatagg cgttggccac
     1741 ggtctgggct tcaactccct gggcggcatc cacgatgagt aaagcaccct cacaggcagc
     1801 aagggacctg gagacctcgt aggtgaaatc cacatggccg ggcgtgtcga tgaggttcag
     1861 gacgtaatcc aggccatcgg aagccctgta cttcatctgc accggcacga gtttgatcgt
     1921 gatcccccgc tcgcgttcaa gctcgagggt atcgaggatc tgttcgcgca tctccctggc
     1981 actgacagtg gtcgtagcct cgagcaggcg atcggcgagg gtcgatttcc cgtggtcaac
     2041 gtgggctatg atgcagaagt tcctgatgcg ttctatgggc accttcatta tgagacacct
     2101 ctggcttgag aaacgaaacc aattctaacc aacaggaagg gaaatgtcat cggtgatggg
     2161 cgtttgaagg aacacggacg gccggattcg gagaattcaa gggcaccggt gcctttcctc
     2221 gagccaggat cgaatgtctt ccggggtatt aacgt
//
LOCUS       DAOSRG010000199         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_44021_length_2246_cov_8.990872, whole genome shotgun sequence.
ACCESSION   DAOSRG010000199 DAOSRG010000000
VERSION     DAOSRG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44021_length_2246_cov_8.990872"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLM30_06085"
     CDS             <1..797
                     /locus_tag="PLM30_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54774.1"
                     /translation="GETVGANGTPDWRITLSLAGEGTVISFIVRGIEGSVAEWDTLPG
                     NKKPLVVVTDQDGKILNASNGSISIPLAGRKDLSLWLDDAGALSEPANKFRVIAVLSD
                     GRTFERLIERPSPHLPAPKGAGEVMESDSVRPFYMGKGPRNILGKGEPADITKGLDAN
                     TDAHVRLRLASLDGTIESLTVESLDGQGGDWDTIPGNGRWHIAVTETATGPVLNKNDG
                     SFTRKISGSSELHLWLADNGKLSANPSGYRVVVRFSDGRALSQNLF"
     gene            complement(850..1779)
                     /locus_tag="PLM30_06090"
     CDS             complement(850..1779)
                     /locus_tag="PLM30_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR54775.1"
                     /translation="MRLLGFACVILAALFFGSLGPAERVAYQYGVSPAGVSLVRVVTG
                     ALVAGSCAIFKDPRALVPGFADGWKYALSGLFGVVFVYYLSNIAFVTIPVGLTVILFY
                     INPVWTALGAAALGKEPFTRIRFMAMAAGLAGVWVAVGGIGGASVGELDMTGVALAVV
                     SGAGFSFFILNGRYGTGREEPFKTFVQMFLWGSLMMGVIAFSRGEVPNLRGLPLPALL
                     ALAHLALLPTLASYGLISFALKHIPGTAASITSMSEIVFAGVMAWVFLGEAPAAGQVK
                     GALLIVTAVLLLVLEGKLSFTGEGEGGKKGTSP"
     gene            1973..>2246
                     /locus_tag="PLM30_06095"
     CDS             1973..>2246
                     /locus_tag="PLM30_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HOR54776.1"
                     /translation="MGLPYIARIEHYEPFIGGEAVERILSKASKMSGRRIVHINSTFY
                     GGGVAELLGSMTLLANLAGIQMGWRVIQGSPDFFTVTKKMHNALQGD"
BASE COUNT          477 a          718 c          710 g          341 t
ORIGIN      
        1 ggggcgagac cgtcggcgcc aacgggactc ccgactggag gatcaccctc tccctggcgg
       61 gcgaagggac cgtgatcagt ttcatagtcc gggggatcga gggttccgtg gccgagtggg
      121 acaccctccc gggcaacaag aaacccctgg tggtggtcac cgaccaggac ggcaagatcc
      181 tcaacgcctc caacggttcg atctcgatcc ccctggccgg caggaaggac ctctccctct
      241 ggcttgacga cgcgggggct ctttcggaac cggccaacaa gttcagggtc atcgcggtcc
      301 tgagcgacgg gaggaccttt gaaaggctca tcgaacggcc gtcgccccat ctcccggccc
      361 cgaagggcgc gggggaggtc atggaatcgg acagcgtcag gcctttctac atgggtaagg
      421 ggccgaggaa catcctcggc aagggggaac cggcggacat aaccaagggc ctcgacgcga
      481 acaccgacgc ccacgtgagg ctcaggctcg cgtccctcga cgggaccatc gagtccctga
      541 cggtggaatc cctcgacgga caggggggcg actgggacac catccccggc aacggcaggt
      601 ggcatatagc ggtcaccgag accgccacgg ggccggtgct gaacaagaac gacggttcct
      661 ttaccaggaa aatctcgggg agcagcgaac tccacctttg gctggccgac aacggcaagc
      721 tgagcgccaa cccctcgggc tatagggtcg tggtccgctt ctccgacggc cgggccctct
      781 cacagaacct gttctaggga ccgacctttt tcggggggtt tttcgcaacg ggtcattccc
      841 gggacctcct taaggggagg tccctttttt ccctccttcc ccctcacccg taaaggacag
      901 tttcccttcg aggacgagga gcagcacggc cgtcactatc agcagcgccc ccttcacctg
      961 ccccgccgcg ggggcctccc cgaggaagac ccaggccatg acgccggcga agacgatctc
     1021 cgacatggag gtgatcgagg ccgccgtccc ggggatatgc ttgagagcga aggatataag
     1081 gccgtaggat gccagcgtgg gcagaagggc caggtgggcc agcgccagca gtgcggggag
     1141 gggaagaccc cgcaggttgg gaacctcgcc tctggaaaag gcgatgaccc ccatcatgag
     1201 cgaaccccaa aggaacatct gcacgaaggt cttgaagggt tcttccctcc cggtcccgta
     1261 acggccgttg aggatgaaga aggaaaaacc agcccccgag acgacggcaa gggccacgcc
     1321 cgtcatgtcc agctccccca cgctcgcccc gccgatgccg ccgacggcga cccacacgcc
     1381 ggcgaggccg gccgccatgg ccatgaaacg gatcctggtg aagggctcct tgcccagggc
     1441 ggcggccccc agggccgtcc atacggggtt tatatagaaa aggatgaccg tcagccccac
     1501 cggtatggtc acgaaggcta tgttcgacag gtagtaaacg aagaccacgc caaaaaggcc
     1561 cgacagggcg tacttccagc catcggcgaa gcccggcact agggcccgcg ggtccttgaa
     1621 aatcgcgcag gatcccgcca caagggctcc tgtgacgacc cggacaaggg agaccccggc
     1681 gggagatacg ccgtactggt aggccaccct ctcggcgggg ccgagggatc cgaagaaaag
     1741 agcggcgagt ataacgcagg cgaaccccaa cagtctcaag gcgacaaccc cttctataca
     1801 cgacctgagg accttcccgc ccggatcgcc tggggatcgg cgggaggaga gccgattaga
     1861 atgatttatc ctatataata ggacacaaac gggatgaaac ggtcgccggc gtcaaggata
     1921 ggatcgtttc cgcccgccac cggcggcgtt tccttaaaaa gggggagacc ccatggggct
     1981 tccgtacatc gccaggatcg agcactatga acccttcatc gggggtgagg ccgtcgagag
     2041 gatcctctca aaggcctcaa agatgtcggg ccgcaggatc gtccacatca actccacctt
     2101 ttacggcggg ggtgtggccg aactgctggg ttccatgacc ctcctggcca acctggcagg
     2161 catccagatg ggctggaggg tcatccaggg ctcccccgac tttttcaccg tcacgaagaa
     2221 gatgcacaac gccctccagg gcgatg
//
LOCUS       DAOSRG010000200         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_44152_length_2241_cov_10.194419, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000200 DAOSRG010000000
VERSION     DAOSRG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44152_length_2241_cov_10.194419"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLM30_06100"
     CDS             complement(<1..391)
                     /locus_tag="PLM30_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54777.1"
                     /translation="MDREKRSYRWIWWLLLILLVALGTWRIFFRPASQPPVSVAEIRS
                     REGVPVTVHDVAPSRWEHWITFFGKTKAVSEVQVAAERQEYVSSVVVDVGDSVKTGQP
                     LATLDTRVARQRLAAQKASVTEKQGRYR"
     gene            complement(394..1692)
                     /locus_tag="PLM30_06105"
     CDS             complement(394..1692)
                     /locus_tag="PLM30_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR54778.1"
                     /translation="MTRMTPLFRMRRTPASILFAAGIAFLLFSSPGKALAGPVTWEQA
                     WGLILENNPGLKTARQNISAAHAALKLAAVPTKVTAGLSGTTTKNEGVESSSSAGLTL
                     SYNVGLLGREKEAIEAETAGLREAEVAYRAAVLKLYRNSALAFWGAAAGEASVKAAEE
                     EILKREAFLRDARLRYVQGMVPELDVMRAESALAESRHSLALRQASRSGYEAMLKGLA
                     GWMDIQPVEGLFDTGSPVGAGRERPDFESVARDHPSVLKAKWAVRRAEHLLKVAGMAS
                     QPTLGLSATMTLVTDGAASLQYNEDEWWWRATLTIPVVDGGYARWSAERARAALASTE
                     AAVGSATAEVMMDLMSAWEDYSAAMEGYEAERARFDTVSREREIVLLRYREGLANQIE
                     VLDAQTRFAGSQATLIDARRGLLVAEAALASAEGKLPMEE"
     gene            complement(1778..>2241)
                     /locus_tag="PLM30_06110"
     CDS             complement(1778..>2241)
                     /locus_tag="PLM30_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOR54779.1"
                     /translation="RVAGEEVAVRAKIHTINGFSAHADRRDLLAWARQYRTDPLFFIT
                     HGEQGSSLALAKTFDENGIRSFIPEEGGEYSLVGNNGTARPGVLGTRAVVAVAGEAPG
                     RRTIDAVLGDIVTLAAELREDGSSLPREEALALALSARTLLKTLKRRGAND"
BASE COUNT          398 a          756 c          672 g          415 t
ORIGIN      
        1 gcctgtacct gccctgtttt tcggtgaccg aggccttctg cgccgccagt ctctgtctgg
       61 cgacgcgcgt gtccagcgtc gctaggggct gccccgtttt cacggagtcc cccacgtcga
      121 caaccacgga ggagacatat tcctggcgct cggcagctac ctggacctcg gagaccgcct
      181 tggtcttgcc gaagaaggtg atccagtgct cccatcggga gggggcgacg tcatgcaccg
      241 taaccgggac gccctcccgg gaacggatct ccgcgacgct caccggcggc tgggaggcgg
      301 gtctgaagaa gatcctccag gtaccgagag caacgagcaa tatcaggagt agccaccaga
      361 tccaccggta agaacgcttt tccctgtcca cttctattcc tccatcggca gcttcccctc
      421 ggcggaagca agggccgctt ccgcgacgag gagtccccgc cttgcgtcga tgagtgtcgc
      481 ctgggagccg gcgaaacggg tctgcgcgtc gaggacctcg atctggttcg ccagtccctc
      541 cctgtaacgg aggaggacga tctctctttc cctggacacc gtatcgaacc tggccctctc
      601 ggcttcgtat ccctccatgg cggcgctgta atcctcccag gccgacatga ggtccatcat
      661 tacttcggcg gtggcggatc caacggccgc ctcggtcgag gcgagggcgg cccttgccct
      721 ctccgcggac caacgggcgt acccgccgtc caccacgggt atcgtcagag tggccctcca
      781 ccaccattca tcctcgttat actggagtga cgccgcaccg tcggtgacga gggtcatcgt
      841 cgccgacaac cccagggttg gttgggatgc cataccggcc accttgagga ggtgttcggc
      901 cctccggacg gcccactttg ccttgaggac cgaggggtga tcgcgcgcga cggactcgaa
      961 gtccggccgc tcacgccccg ccccaacggg ggaacccgtg tcgaaaaggc cctcgacggg
     1021 ttgaatatcc atccagcccg ccaggccttt cagcatagct tcgtaccccg acctggaggc
     1081 ctgccgcaag gccagggaat ggcgggattc cgccagggcg ctctcggccc tcatcacatc
     1141 cagctccggg accatgccct ggacgtatcg aagccgggca tcccttagga aggcttccct
     1201 cttgaggatc tcctcctcgg ccgccttgac cgaagcctca cccgcggcgg cgccccaaaa
     1261 agccagggcg gaattcctgt acagtttcaa aacggcggcc ctgtaggcga cctcggcctc
     1321 cctgagcccc gccgtttcag cctcaatggc ttccttctcc ctacccagga gtccgacgtt
     1381 gtaagagagg gtgagacctg cgcttgaaga cgattccacc ccttcattct tcgtagtcgt
     1441 ccctgaaagg cccgcggtga ccttcgtcgg gacggccgcc agcttcaatg cggcatgggc
     1501 cgcggatatg ttctgcctgg cggtcttcag cccggggttg ttctccagta tgagtcccca
     1561 ggcctgttcc caggtcaccg gccccgccag ggccttgcca ggggatgaaa ataaaagaaa
     1621 ggcaatgccc gccgcgaaaa ggatcgaggc gggggttctt ctcatccgga aaaggggggt
     1681 catcctagtc atcaccgcca gtcttccaaa aaaggaccca cagaattcta gcacagcgag
     1741 cggccaggcg cgcctttgga atggtcccgg gaagggacta gtcattcgct cctcttcgtt
     1801 tcaacgtctt cagcaatgtc ctggcggaga gggcgagggc cagggcttcc tcccgcggaa
     1861 gagaggaccc atcctccctc agttccgcgg cgagggtgac gatatcgcca agcacggcat
     1921 cgatggtccg gcgccctggg gcctcaccgg cgacggcgac aaccgcccgg gtccccaaca
     1981 cccccgggcg ggcggtccca ttatttccga ccaggctgta ctccccaccc tcctcaggta
     2041 tgaaagatct tatgccattt tcatcgaagg tcttcgcgag agccagggat gatccctgtt
     2101 caccatgggt gatgaagaac agcgggtcgg tccggtactg cctggcccag gcaagaaggt
     2161 cacggcggtc ggcgtgggcg gagaaaccgt tgatggtgtg tatcttggcc ctgacggcca
     2221 cctcctcccc ggcgacccgg a
//
LOCUS       DAOSRG010000201         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_44678_length_2223_cov_8.424815, whole genome shotgun sequence.
ACCESSION   DAOSRG010000201 DAOSRG010000000
VERSION     DAOSRG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44678_length_2223_cov_8.424815"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /gene="ortA"
                     /locus_tag="PLM30_06115"
     CDS             <1..276
                     /gene="ortA"
                     /locus_tag="PLM30_06115"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOR54780.1"
                     /translation="LGVGERAPQVPGDTAAVPLEMTVRGTLVDAFAEPGEEVTVETPA
                     GRRLRGRLVGLEPAYDIGFGPPPPELRPVGPELRKILSGEVIEREKT"
     gene            263..1696
                     /gene="ortB"
                     /locus_tag="PLM30_06120"
     CDS             263..1696
                     /gene="ortB"
                     /locus_tag="PLM30_06120"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOR54781.1"
                     /translation="MKRPKDYSSVMARRGEIMRRSVGIDYGSFATGRLAFDYEGMMEE
                     TGYTLDEVKKIQKGSNVGDTPLLELKNLTALARRISEPGRGARIFVKDEANNPARSFK
                     DRRASLSVHRALERGYPGVVAATSGNYGSAVASQAARENLPCIIVQEVFDSRGFEQPE
                     IAEKGRACEAYGAEVLQLSVGPELFYVFLLVLEETGFFNASLYTPFSIAGIETLGVEI
                     VEQTREMAGRPPDAIVSTHAGGGLTTGTARGVLKAGSPETMIIGASVNLEGLHMASDR
                     DFNRKSFTTGHTGFGIPFAVQPDRSDVPRNAARVLRYLDRYVTVTQGSVFYMTEALAQ
                     LEGLERGPAGNTSLAAAFSLARTMGEDEVIVVNETEYTGAGKHPYAQIAFARKMGVEV
                     FRGDPSMERPGENIVIPLEPGQVTAAEVDLDRLRGSYIKNACKSLGEDKVISEVDLDF
                     LCEDARLDRGRALELLAAEGVRISALG"
     gene            1760..>2223
                     /locus_tag="PLM30_06125"
     CDS             1760..>2223
                     /locus_tag="PLM30_06125"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR54782.1"
                     /translation="MEVSLDNLRYNYRLIKEHVKGARVIAVVKANAYNLGAVQAAWAL
                     RGAGADFFAVATPDEAIELRDGGITSPVLVLGTSPYSVAGEYVRLGIRAAITDPAMAR
                     ALSAEAVRQGKKALVHIKIDTGLGRIGFTTDQVLAAVEDIVALPGVEAEGVFT"
BASE COUNT          430 a          687 c          747 g          359 t
ORIGIN      
        1 ctcggggtcg gcgaaagggc cccccaggtc ccgggtgaca cggcggcggt gccgctggag
       61 atgaccgtaa gagggaccct cgtggatgct ttcgccgagc ctggagagga ggtcaccgtg
      121 gagaccccgg cgggccgcag gctgaggggg aggctggtcg gcctggagcc ggcctacgat
      181 atcggtttcg gaccgccgcc gccggaactc cggccggtgg gtccggaatt gaggaagatc
      241 ctctccgggg aagtgatcga acgtgaaaag acctaaggat tattcctccg ttatggcccg
      301 acggggcgag atcatgaggc gctccgtggg catcgattac gggtccttcg ccaccggcag
      361 gctggccttc gactacgaag gcatgatgga agagaccggc tacaccctcg atgaagtaaa
      421 aaagatccag aagggatcca acgtcggcga tactcccctc ctggaactga agaacctcac
      481 cgccctggcc aggaggatct ccgaacccgg caggggggcc aggatcttcg tcaaggacga
      541 ggccaacaac cccgcccgct ccttcaagga ccgtagggcc tccctctcgg tccaccgcgc
      601 cctggagagg ggctaccccg gggtcgtggc cgccaccagc ggaaactacg ggtccgccgt
      661 ggccagccag gccgcgaggg aaaacctccc ctgtattatc gtccaggaag tattcgacag
      721 cagaggtttc gagcagccgg aaatagccga gaaggggcgc gcctgcgagg cttacggtgc
      781 cgaggtgctc cagctctccg tcgggcccga gcttttttac gtcttcctgc tggtcctgga
      841 ggaaacgggc tttttcaacg cctcccttta cactcccttt tccatcgccg gcatcgagac
      901 cctcggcgtg gagatcgtgg aacagaccag ggaaatggcg gggcggccgc ccgacgccat
      961 cgtcagcacc cacgccgggg gcggactcac cacagggacc gccaggggcg tgctgaaggc
     1021 cggaagcccc gaaacaatga tcatcggtgc cagcgtcaac ctcgaggggc tccacatggc
     1081 ctccgacagg gatttcaacc gcaaatcctt caccacgggg catacggggt tcggcattcc
     1141 cttcgccgtc cagcccgacc gctccgatgt ccccaggaac gccgccagag tgctccgcta
     1201 cctggaccgg tacgtgacgg tcacccaggg gtcggtcttc tacatgacgg aagcgctggc
     1261 acaactggag ggcctcgaaa gggggcccgc cgggaacacc tccctcgcgg cggccttctc
     1321 cctggcccgc accatgggcg aggacgaggt gatcgtcgtc aacgagacgg agtacaccgg
     1381 cgcgggcaag cacccctatg cgcagatcgc cttcgcgagg aagatgggcg tcgaggtctt
     1441 ccggggggac ccctcgatgg aaaggcccgg ggagaacata gtgatcccct tggaacccgg
     1501 gcaggtgacc gccgccgagg tggacctgga caggctgcgg ggttcctata tcaagaatgc
     1561 ctgcaagagc ctcggtgagg acaaggtaat ttcagaggtc gatctcgact tcctttgcga
     1621 ggacgccagg ctcgacaggg gaagagcact ggaactcctg gcggcggagg gcgtgaggat
     1681 ttcggccttg ggttaggagc tcggctccta cttttgaaaa gaaaggaagg atgtcccgat
     1741 gggatggcgg cccacgagga tggaagtaag tctcgataat ctccgttaca actacaggct
     1801 cataaaggag cacgtcaagg gcgcccgggt gatagcggtg gtgaaggcca acgcctacaa
     1861 cctgggcgcc gtccaggcgg cctgggcgct tcggggggcc ggtgcggact ttttcgccgt
     1921 ggcgaccccc gacgaggcca tcgagttgag ggatgggggg ataacgagcc ccgtcctcgt
     1981 cctgggtacc tccccctatt ccgtcgcggg ggaatacgta cgcctgggga tccgggcggc
     2041 cattaccgac ccggcgatgg ccagggccct ctcggcggag gctgtgaggc agggcaaaaa
     2101 agccctggtg catatcaaga tcgacaccgg cctggggcgc atcggtttca ccaccgacca
     2161 agtccttgcg gccgtcgagg atatcgtcgc ccttccgggc gttgaggccg aaggcgtctt
     2221 cac
//
LOCUS       DAOSRG010000202         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_45559_length_2191_cov_8.468165, whole genome shotgun sequence.
ACCESSION   DAOSRG010000202 DAOSRG010000000
VERSION     DAOSRG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45559_length_2191_cov_8.468165"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLM30_06130"
     CDS             <1..951
                     /locus_tag="PLM30_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR54783.1"
                     /translation="LNDRTRVKGRWNGWVPGAGELLPEVIEGILCRALGEEPPVGAPE
                     ELRKAAEELGITPRKPMLCPGCPHRASFFSIMQALPRAKTIYPSDIGCYTLAVNQGMV
                     DSVVDMGASITQSSGLFMAYRADGKEQPIVATIGDSTFFHMGLPGLVSAVYNRHAFVL
                     AILDNSLTAMTGGQSHPGIGDKLRKGEAGRKVDLEGAVRGCGVNWVRTTEAYDISASR
                     VLVKEAWEHARTHGEPAVVIFRHPCILLRQKQAVIPVTVDPEKCIGCRFCIDFFNCPG
                     LVFDDKTKKAYIDERFCINCGVCINVCPHGAILAVEGEEA"
     gene            954..1511
                     /locus_tag="PLM30_06135"
     CDS             954..1511
                     /locus_tag="PLM30_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR54784.1"
                     /translation="MQYVIVGIGGQGILFSSRVLGHIALSRGEGVIGSEVHGMAQRGG
                     SVISHFKMGDHLSPLVKAGDADVLLAFDQNEAVRNLHFLKQGGSAVVNTHDPSFLENP
                     SLGKWLAERKVRVYPVKGYDILKAEMGGRFLFLNVLILGALCGAGVGDVTIAEVREAV
                     KALAPSKFVDDNLKAVDLGYAGTHR"
     gene            1462..2031
                     /locus_tag="PLM30_06140"
     CDS             1462..2031
                     /locus_tag="PLM30_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR54785.1"
                     /translation="MTTSKRWTWAMPEPIDEEIEGPSRGAGKGLPAFWSPRSRRAFTL
                     VEAVVVLSLMAALSLTFYPIMAGLLSQRAGSEQAARAEARKLTRHLERTLMRALLHRR
                     AFDVQYLSGFRKEFLVHWYNPSEYERYQTNGRCLVYFKSTAAQSKCYTPTWHTMTPAF
                     TLYIYTPGKKKVAEIAVSGYCSVRLTELP"
     gene            2068..>2191
                     /locus_tag="PLM30_06145"
     CDS             2068..>2191
                     /locus_tag="PLM30_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOR54786.1"
                     /translation="MAAKPWWRETIETILWALVLALVLRALVIQAFWIPSGSMIP"
BASE COUNT          433 a          649 c          698 g          411 t
ORIGIN      
        1 ctcaatgacc ggaccagggt caaggggaga tggaacggct gggtccccgg cgcgggggaa
       61 ctgctgccgg aggtgatcga agggatcctt tgcagggccc tgggcgagga gcctcccgtg
      121 ggagccccgg aagaactcag gaaggccgcc gaggaactgg gcatcacgcc ccggaagccg
      181 atgctctgcc ccggttgccc ccacagggcg agtttcttct ccatcatgca ggccctgccg
      241 agggccaaga ccatataccc ctccgatatc gggtgttaca ccctcgccgt gaaccagggc
      301 atggtcgact ccgtggtcga tatgggcgct tccataaccc agtcctcggg tcttttcatg
      361 gcctacaggg ccgacggcaa ggaacagccc atcgtggcca ccatcgggga ttccaccttc
      421 ttccacatgg gtctccccgg cctcgtcagc gccgtctaca acaggcacgc cttcgtcctg
      481 gccatactgg acaacagcct cacggccatg acgggcggcc agtcccaccc gggcatcggc
      541 gacaagttga ggaaaggtga agcggggcga aaagtcgacc tggagggagc cgtcagggga
      601 tgcggcgtca actgggtcag gaccaccgag gcctacgata tctcggcttc gagggttctc
      661 gtcaaggagg cgtgggagca cgccaggacc cacggtgagc cggcggtagt catcttccgc
      721 cacccctgca tactgctccg gcagaagcag gcggtgatac ccgtcacggt ggacccggaa
      781 aagtgcatcg gctgcaggtt ctgcatcgac ttcttcaact gccccgggct ggtcttcgac
      841 gacaagacca aaaaagccta catcgatgaa aggttctgca taaactgcgg tgtctgcata
      901 aatgtctgcc cccacggggc catcctcgct gtggaagggg aggaagccta gccatgcagt
      961 acgttatcgt cgggatagga ggacagggga tacttttttc aagccgggtc ctcggccaca
     1021 tcgccctctc caggggtgag ggcgtcatag gcagcgaggt ccacgggatg gcccagcggg
     1081 gcggttcggt gataagccac ttcaagatgg gcgatcactt aagccccctg gtcaaggccg
     1141 gcgacgccga tgtcctcctg gccttcgacc agaacgaggc cgtgaggaac ctccacttcc
     1201 tgaagcaggg tgggagcgcc gtcgtcaaca cccacgaccc ttccttcctg gagaacccct
     1261 ccctggggaa gtggctggca gagcggaagg tccgggttta ccccgtaaag ggttacgata
     1321 tccttaaagc cgagatggga ggccgtttcc tcttcctgaa cgtcctcatc ctgggtgctc
     1381 tttgcggcgc cggggtgggt gacgtgacga ttgcagaggt gcgcgaagcg gtcaaggccc
     1441 tggctccatc gaaattcgtc gatgacaacc tcaaagcggt ggacctgggc tatgccggaa
     1501 cccatcgatg aggaaataga aggaccgtcg cggggggccg gaaaggggct ccccgctttt
     1561 tggagcccgc ggtcaagaag agccttcacc ctggtggagg ccgtcgtggt gctctccctc
     1621 atggctgccc tgtccctgac cttttacccg atcatggcgg ggcttttgtc ccagagggca
     1681 ggcagcgaac aggcggccag ggccgaggcc aggaaactaa cgaggcacct ggagaggacc
     1741 ctcatgaggg ccctcctgca ccgcagggct tttgacgtac agtatctgtc gggtttcagg
     1801 aaggaattcc tggtccattg gtacaacccc tcggagtacg agcgatacca gacgaatggc
     1861 cggtgcctag tttacttcaa gtcgaccgcg gcgcagtcca agtgttatac tccaacgtgg
     1921 catacgatga ccccggcttt caccctgtat atctataccc caggcaagaa gaaggtcgca
     1981 gaaatagccg tgtcgggcta ttgctccgtg aggctcacgg agttgccctg aggcccggcg
     2041 gggatttttg atttgggggg ctttatcttg gcggcgaaac cttggtggcg agagacgata
     2101 gagaccatcc tttgggccct tgtgctggca ttggtactga gggcgctggt gatacaggcg
     2161 ttctggatcc ccagcggctc catgatcccg a
//
LOCUS       DAOSRG010000203         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_45622_length_2189_cov_9.947516, whole genome shotgun sequence.
ACCESSION   DAOSRG010000203 DAOSRG010000000
VERSION     DAOSRG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45622_length_2189_cov_9.947516"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLM30_06150"
     CDS             complement(<1..892)
                     /locus_tag="PLM30_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR54787.1"
                     /translation="MFLKKSIPIQHKIMLLIMSLVSVTLVAAGVFFERGIIPSFEEKQ
                     ALHARDIAYTVSQIPLVKEQIDRPNHKKVTQPILDNIIKGTNVKLILLIGGRDALPVI
                     EGIPFSTLPEVTTFGPIVRAYLPVHRDGAKVGAVAVYLWSRDIKTKTWELRKKIILSV
                     IFGLILGTFFAHLLSKNIKRSMLGMEPYQLAALLKEREAFLETVREGIIAIDRDGKIM
                     FVNQQAKEILGVPESEIMVGHLIEKYVPNSRLRRVLASGEPEYDREQNLGGARIFTNR
                     IPIRSGNRILGAIASFRKADE"
     gene            1208..>2189
                     /locus_tag="PLM30_06155"
     CDS             1208..>2189
                     /locus_tag="PLM30_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOR54788.1"
                     /translation="MARRTSVLLVAVFVFCAVLGMAFVSQRADAAEIKLRAGHEMPEN
                     HPYHLGTVRFGELLKEKTNGRIEVVVYPNGQLGNQTQLAQMVAANQLDVCLVWQGVLE
                     SFESNVGVVSLPYIFDSWEHTWKVVDGELGQSIFAPLEAKGIKVITVFNNGLYNVVSR
                     VPVKTPEDMKGIKLRVQPSAVFTETGEMLGSVVTPMAFGEVYSALQLGAIDAGILGPI
                     NIRKSKHYEVAKFTCQNEIHYLLEPMMMSAKAFKALSTEDQKAVLEAAKEAAEWQRVE
                     AEKEDASDTAFLKENGMHYFVPDKDEWRAAVMPVYDKYPQWKEMVEKINKL"
BASE COUNT          454 a          617 c          648 g          470 t
ORIGIN      
        1 tctcgtcggc cttccggaaa ctcgctatgg cccccaggat cctgttcccg gaccgtatgg
       61 ggatacggtt ggtgaagatc ctggcccccc ccaggttctg ttcccggtcg tattcgggtt
      121 cccccgaggc caggacccgc cggagcctgg agttggggac gtacttttcg atcaggtgcc
      181 ccaccatgat ctccgactcc gggaccccga ggatctcctt ggcctgctgg ttgacgaaca
      241 tgatcttccc gtcccggtcg atagcaatta tcccttccct tacggtctcc aggaaggcct
      301 cacgctcctt gaggagggcc gccagttggt agggttccat gccgagcatg gacctcttga
      361 tattcttcga gaggaggtgg gcgaaaaagg tgcccaggat gagcccgaaa atgacggaga
      421 ggatgatctt tttgcgcaat tcccaggtct tggtctttat atcccgggac cagaggtaaa
      481 ccgccaccgc cccgaccttt gccccgtccc tgtggacggg aaggtaggcc cttacgatcg
      541 gcccgaaggt ggttacctcc gggagcgtcg aaaaggggat accctcgatc acgggtaggg
      601 cgtcccgtcc gccgatgagg aggatgagct tcacgttggt gcccttaatt atgttgtcca
      661 ggatgggttg ggtcaccttc ttatggttcg gcctgtcgat ctgttccttg accaggggta
      721 tctgcgaaac ggtgtaggcg atatccctgg cgtggagcgc ctgcttctcc tcgaaagaag
      781 gtatgatccc cctctcgaag aacaccccgg ccgctaccag ggtgacgctg acgaggctca
      841 tgatgaggag cattatcttg tgctgaatag gtattgactt cttaaggaac aatccttcgc
      901 ctccaggtgt tgatgtgaaa ggttccccct caagccgggc cggggcgcgc ccgtcctctt
      961 accgctgata cccgaagcgt agcgccttgg cccggctttc gtaacttgcg aagcgtgtta
     1021 ataaagtaca aaaggctttt accctgcccc aataaatata tcattggttc cggaacgcgc
     1081 acgatccagg gccttcggac tcggatccag ccgaaagcgg tgagacccat tcgggggagt
     1141 ggaggtgatc cgcggaggga aggcgaatcg ttccgttggg caaaccatat tacaggaggg
     1201 ataggcaatg gcacgaagga cttccgtttt gcttgtagcc gtgttcgttt tttgcgctgt
     1261 tttgggtatg gcctttgtct cgcagcgggc tgatgccgct gagatcaagc tgagagccgg
     1321 ccacgagatg cccgaaaacc acccctacca cctggggacc gttcgtttcg gcgaactgct
     1381 gaaggaaaag accaacggcc gcatcgaggt cgtggtctac ccgaacggac agctgggcaa
     1441 ccagacccag ttggcccaga tggtcgccgc caaccagctc gatgtttgcc tcgtgtggca
     1501 gggcgtcctg gaatcctttg aatccaatgt gggcgtcgtc tcactccctt atatcttcga
     1561 cagctgggag catacctgga aggtcgtcga cggggaactc ggccagtcca tttttgcccc
     1621 ccttgaggcc aaggggatca aggtcatcac ggtgttcaac aacggccttt acaacgtcgt
     1681 ctccagggtc ccggtcaaga ccccggaaga catgaagggg atcaagctcc gcgtgcagcc
     1741 ttccgcggta ttcactgaaa ccggtgagat gctgggttct gtcgtgactc ccatggcctt
     1801 cggcgaggtc tattcggccc tgcagcttgg ggccatcgat gcaggaatcc taggaccgat
     1861 caacatcagg aagagtaaac actacgaagt ggccaagttc acatgccaga acgagatcca
     1921 ctacctcctt gaacccatga tgatgagcgc caaggccttt aaggccctct cgaccgagga
     1981 tcagaaggcc gtccttgagg cggctaagga agcggccgaa tggcagcgcg tcgaggcgga
     2041 gaaggaggat gcctccgaca cggctttcct caaggagaac ggcatgcatt actttgtccc
     2101 tgacaaggac gagtggagag cggccgtcat gcccgtgtac gacaagtatc cccagtggaa
     2161 ggaaatggtc gagaagatca acaagctta
//
LOCUS       DAOSRG010000204         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_45758_length_2184_cov_10.860498, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000204 DAOSRG010000000
VERSION     DAOSRG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45758_length_2184_cov_10.860498"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PLM30_06160"
     CDS             <1..1109
                     /locus_tag="PLM30_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR54789.1"
                     /translation="SGQGPFFEQILDLPQGGKHWLRWTRRGILDEKGDLIEVQAVGRD
                     VTILKRTEEELRQLNSFLRSVRGVNRLISRHHDSEKLVQEICAHLVETRGYRNAWIGI
                     LGKGGTLQSVSEAGVTADGKHLSERRKHGMLNEWGLSALEGPGIHLKRGTMTMTDSPL
                     LDSYEEGGIMTTRLEYAKKIYGLVSVSLPGDAHFDPEEQTLFSELAEDISFALHAIET
                     EKDRQRKAANLEEKARQLELLVAEHSSGLWEWDVSEGRVVMNAAYETMLGYSPGEMGQ
                     SLDDWKRQIHPDDQAGVADLLRRVLSGKDGDDIYETEFRMLKKDGSWLPLRSRGFVVR
                     RDKNGRAAKMVGIHTPAGEALQKQRRRRGHEAKG"
     gene            1093..1557
                     /locus_tag="PLM30_06165"
     CDS             1093..1557
                     /locus_tag="PLM30_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOR54790.1"
                     /translation="MRRKDKEITDEAQIRGILERGKVCRVAFFDEEYPYMVPLCFGFD
                     GRALYFHGFNGGKKIECLHKNPKVCFEVDEDIEVVQDPDPCEWTMKYRSVVGFGTAVF
                     LEETEEKREGLNHVVKQYAGRELPFPDQAMAAIAVVRIDIESMTGRRNGYVL"
     gene            1572..>2184
                     /locus_tag="PLM30_06170"
     CDS             1572..>2184
                     /locus_tag="PLM30_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOR54791.1"
                     /translation="MLGQVVTWIVSTIGRMGYTGVVALMFLESSFVPFPSEVVVPPAG
                     YLAAKGQMNLFLVILAGIAGSILGAIFNYWISLTFGRPFFERYGKYFLVSDKALERAD
                     VYFARHGHISTFIGRLLPGIRQLISLPAGLTRMNIPLFLFYTTLGSGIWVVTLAWVGF
                     WVGNNQALVHRYMHTASYVLAAFCVTLAAAYVFHCRRKNKKIEK"
BASE COUNT          464 a          659 c          694 g          367 t
ORIGIN      
        1 cctcgggcca ggggcccttc ttcgagcaaa ttctcgacct cccccagggg gggaagcact
       61 ggctgagatg gaccaggagg ggcatcctcg atgaaaaggg cgacctcatc gaggtccagg
      121 ccgtgggccg cgatgtgacc atcctcaaga ggaccgagga ggagctgagg caactgaact
      181 ccttcctccg ctccgtcagg ggggtaaacc ggctcatcag ccgccaccac gatagcgaga
      241 agctcgttca ggagatctgc gcccacctgg tggagaccag gggctaccgc aacgcctgga
      301 tcgggatcct gggcaaggga gggaccctac agtccgtatc ggaagcgggc gtgacggccg
      361 acggcaaaca cctgtcggag aggagaaagc acggcatgct caacgaatgg ggactttcgg
      421 ccctcgaggg accggggata cacctgaaac ggggcaccat gaccatgacc gacagccccc
      481 tcctggatag ctacgaagag ggtggcatca tgaccaccag gctggaatac gccaaaaaga
      541 tctacggcct ggtatcggta tccctccccg gcgacgccca cttcgacccc gaggagcaga
      601 ccctcttctc ggaactggct gaggatatat ccttcgccct ccacgccatc gagaccgaga
      661 aggaccgcca gaggaaggcc gccaacctcg aggaaaaggc ccggcagctc gaactcctgg
      721 tcgccgaaca ctcttcgggg ctctgggagt gggatgtgag cgaggggagg gtcgtcatga
      781 acgccgccta cgagaccatg ctgggctact cccctggcga gatggggcag tccctcgacg
      841 actggaagcg gcagatccac cccgacgacc aggccggtgt ggccgacctg ctgagaaggg
      901 tcctctccgg gaaggacggc gacgacatct acgaaaccga gttcaggatg ctgaaaaagg
      961 atggttcatg gctgcccctc cggagccggg ggttcgtggt ccgcagggat aaaaacggca
     1021 gggccgccaa gatggtcggc atccatacgc cggcggggga agcccttcaa aaacaaagga
     1081 ggaggagagg acatgaggcg aaaggataag gagatcaccg acgaggccca gatcaggggg
     1141 atcctcgaaa ggggcaaggt ctgccgggtg gcctttttcg acgaggagta cccctacatg
     1201 gtccccctct gcttcggctt cgacgggagg gccctctact tccacggttt caacggtgga
     1261 aagaagatcg aatgcctgca taagaaccca aaggtctgct ttgaggtcga cgaggacatc
     1321 gaggtcgtcc aggaccccga cccctgcgag tggaccatga aatacaggag cgtcgtcggt
     1381 ttcggcaccg ccgtcttcct ggaagagacg gaggaaaaaa gggaggggct gaaccacgtg
     1441 gtcaagcagt acgcgggccg cgaacttccc ttcccggacc aggccatggc ggctatcgcc
     1501 gtggtcagga tcgatattga gtccatgacg ggccgccgga acgggtacgt cctttagaaa
     1561 aggcagtggt gatgctcggt caggtcgtaa cctggatagt ctccaccatc gggaggatgg
     1621 ggtacaccgg cgtggtggcc ctgatgttcc ttgagtcgtc ctttgtcccc ttccccagcg
     1681 aagtggtggt cccccccgcg gggtacctcg ctgccaaggg gcagatgaac ctcttcctgg
     1741 tcatcctggc cggcatcgcc gggagcatcc tcggggcgat cttcaactac tggatctccc
     1801 tcaccttcgg gcgccccttc tttgaaaggt acggcaagta cttcctggtc agcgacaagg
     1861 ccctggagcg ggccgatgtg tacttcgcga ggcacggcca catcagcacc ttcatcggca
     1921 gactccttcc cggcatccgc cagctgatat cgctgccggc agggctcacg aggatgaaca
     1981 tccccctctt cctcttctac acgaccctgg ggtcgggcat ctgggtggtg accctcgcct
     2041 gggtcgggtt ctgggtgggc aacaaccagg ccctggtgca ccgttacatg cacaccgcct
     2101 cctacgtcct ggcggccttc tgcgtgaccc tggcggccgc ctacgttttc cactgcagga
     2161 ggaagaataa aaagatcgaa aaaa
//
LOCUS       DAOSRG010000205         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_45795_length_2183_cov_11.370771, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000205 DAOSRG010000000
VERSION     DAOSRG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45795_length_2183_cov_11.370771"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLM30_06175"
     CDS             <1..761
                     /locus_tag="PLM30_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOR54792.1"
                     /translation="MTFHLDFEANLKIVRMCVRHGKRVIFPSTSEVYGMSTDTELRED
                     ESPIILGPVSKSRWIYSCSKQMMDRVIAAYGQEEGLRYTLFRPFNWIGPRLDTFRDAE
                     GRKARSITQILYDVTHGRDVSLVGGGRQRRSFTWVGDGVEALEAVIRNEGGRADGEIF
                     NIGNPKNNASIRELALLVIEALKPHPKYGPLAERARLVEIDSRDYYGKGYEDVLNRVP
                     SVEKAGRLLGWGPRTTLEEAVRMTVEETLAEQGV"
     gene            799..2181
                     /locus_tag="PLM30_06180"
     CDS             799..2181
                     /locus_tag="PLM30_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOR54793.1"
                     /translation="MNVPSHIFREYDIRGNADEELTSETVRAIGLAYGTFLNREGVKK
                     ITLGGDVRLSTERIREDVIQGVTSAGIDVIDIGMVTSPLLYWSLFHLDLDGAVMITGS
                     HNPKEFNGLKLAFGKTTLYGDQIQEIRRMIAADDLVTAVQKGFATKMDLSGEYLAMLE
                     SKISLGGRELKVVTDSGNATAGPLVNRFLRGIGCEVTPLFEEPDGTFPNHHPDPTKRE
                     YLQDLIETVRREKADVGLGFDGDADRIGVIDDRGEIVWGDILMILFWREILPRHPGAE
                     AIVEVKSSQALVDEIKRLGGKVVWWKSGHSLIKAKMKEIDALFSGEVSGHMFFADEFW
                     GFDDAFYAAGRLLRILSNTGRKLSDLLADVPVYHSTAETRVACPDERKFDVIDHIRDD
                     ALKDHEAITVDGVRILYPKGWGLVRASNTQPVIVARTEGKTEGDLAEYSTDLKRRILE
                     AGVEDFDWEY"
BASE COUNT          454 a          638 c          746 g          345 t
ORIGIN      
        1 tgatgacctt ccaccttgac ttcgaggcca acctgaagat cgtccggatg tgcgtgaggc
       61 acggcaagcg ggtgatcttc ccctcgacct ccgaggtcta cggcatgagt accgacacgg
      121 aactgaggga ggacgagagc cccattatcc tgggaccggt cagcaagtcg cggtggatct
      181 acagctgcag caagcagatg atggacaggg tgatcgccgc ctacggccag gaggagggtc
      241 tgcgctatac cctcttcaga cccttcaact ggatagggcc ccggctggat accttcaggg
      301 atgccgaggg aaggaaggcc cgatcgataa cccagatcct ttacgacgtg acccacggga
      361 gggacgtctc cctcgtgggc ggcggccgcc agaggaggag tttcacctgg gtgggcgacg
      421 gcgtcgaagc cctcgaggcc gtcatcagga acgaaggggg ccgcgccgac ggcgagatct
      481 tcaacatcgg caaccccaag aacaacgcct ccatcaggga actggccctc cttgtcatcg
      541 aggcacttaa gccccacccg aagtacgggc cgctggcgga aagggcgagg ctcgtcgaga
      601 tagactccag ggactactac ggcaagggtt acgaggacgt gctcaaccgg gtgccgtcgg
      661 tggaaaaggc cgggaggctc ctgggctggg ggccgaggac caccctcgag gaagccgtga
      721 ggatgaccgt cgaggagacc ctcgccgagc agggagttta aaaaaagggt gttttccttt
      781 tcatcgggag gtgcggccat gaatgtgccg tctcacatct tcagggagta cgacataagg
      841 ggcaacgccg acgaggaact gaccagcgag accgtcaggg ccatcggcct cgcctacggg
      901 accttcctca accgggaggg ggtgaagaag atcaccctgg gcggcgacgt ccgcctctcg
      961 acggagagga tcagggagga cgtcatccag ggggtgacct ccgccggcat cgacgtgatc
     1021 gatataggca tggtcacatc accgctgctc tactggagcc tcttccacct ggacctggac
     1081 ggggccgtca tgatcaccgg gagccacaac ccgaaggagt tcaacgggct caagctggcc
     1141 ttcggcaaga ccacccttta tggcgaccag atccaggaga tccggaggat gatcgccgcc
     1201 gacgacctgg tgacggcggt gcaaaagggc tttgccacga agatggacct ttccggggag
     1261 tacttggcga tgctggagtc caagatcagc ctcgggggaa gggagctcaa ggtggtcacc
     1321 gattcgggga acgccacggc gggccccctg gtcaaccgat tcctcagggg gatcggttgc
     1381 gaggtgaccc ccctcttcga ggaacccgac gggaccttcc cgaaccacca ccccgacccg
     1441 acgaagcggg agtacctgca ggacctcatc gagaccgtcc ggagggagaa ggccgacgtg
     1501 ggcctgggtt tcgacggcga cgccgaccgg ataggcgtca tcgacgaccg gggggagatc
     1561 gtctggggcg acatcctgat gatcctcttc tggcgggaga tactgccccg gcacccgggg
     1621 gcggaggcca tcgtcgaggt caagagctct caggcgctgg tggacgagat aaaacgcctg
     1681 ggggggaagg tggtctggtg gaagtcgggg cactccctga taaaggccaa gatgaaggag
     1741 atcgacgccc tcttctccgg cgaggtgtcg gggcatatgt tcttcgccga cgagttttgg
     1801 gggttcgacg acgccttcta cgccgcggga aggcttttga ggatactgtc caacacgggc
     1861 cggaagctgt cggatctgct cgcggacgtg ccggtctacc acagcacggc ggagacccgc
     1921 gtggcctgtc ccgacgagcg gaagttcgac gtgatcgacc atatccggga cgacgccctc
     1981 aaggaccacg aggccatcac cgtcgacggc gtgaggatcc tctaccccaa gggctggggg
     2041 ctcgtccggg cgtcgaatac ccagcccgtc atcgtcgccc ggacggaggg caaaacggaa
     2101 ggggacctgg cggagtactc gacggacctg aagagaagga tcctggaggc tggcgtggag
     2161 gacttcgact gggaatactg acc
//
LOCUS       DAOSRG010000206         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_45955_length_2177_cov_10.897267, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000206 DAOSRG010000000
VERSION     DAOSRG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45955_length_2177_cov_10.897267"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLM30_06185"
     CDS             <1..212
                     /locus_tag="PLM30_06185"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOR54794.1"
                     /translation="FVEGEPIVGGWDAPEQVPSETSLSRLVSGELKRRGFTFMGPVVV
                     YSHLQAVGLVNDHLRRCFRYGRSSS"
     gene            complement(191..547)
                     /locus_tag="PLM30_06190"
     CDS             complement(191..547)
                     /locus_tag="PLM30_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOR54795.1"
                     /translation="MAIMSLKQFRRSVREAVETVPGELMERLNGGIIVETPIKEEGGY
                     LIMGEYIEDPGLGNIIILYYGSFREAMGGAPLEEWEEEILETVTHELRHHIESLAGVD
                     DLSIEEEHLLQEDDRP"
     gene            687..1898
                     /locus_tag="PLM30_06195"
     CDS             687..1898
                     /locus_tag="PLM30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR54796.1"
                     /translation="MKFSKRVTSMQSSPIRKLVPYAEAAKAEGRKVYHLNIGQPDIVT
                     PPGFLESIKSFAEDVIAYSTSHGSYELIDAMIGYYKGYDIHLERKNLIVTNGGSEALS
                     FALMAICDPGDEVLVPEPFYANYNAFASSVNVKLVPITTKAREGFHFPPKADILKKIT
                     PLTRGILFSNPGNPTGVIYTKEEMEMIADIAMEKDLYVISDEVYREFVYDGLKYTSFG
                     NMKRIEDRVILVDSISKRYSACGARIGCIISKNDELMAQTMKLAQGRLCVPTLEQVGA
                     VALYKTPRSYLEEVNKEYQARRDILYSKIKEMPGVICEEPKGAFYVVAKLPVDNAEKF
                     VIWMLKDFHVDNETVMMAPAEGFYATPNLGRDEVRLAYVLKKEDLTRAMDILKAGLEA
                     YPGRIKTVSNF"
     gene            complement(1980..>2177)
                     /locus_tag="PLM30_06200"
     CDS             complement(1980..>2177)
                     /locus_tag="PLM30_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54797.1"
                     /translation="SAGRIPAWSIEEGDVLVVDRSCQAEKGDLAVITRDGKLALWEVP
                     SGGASTWGVVRGVVRVLRRRE"
BASE COUNT          452 a          701 c          603 g          421 t
ORIGIN      
        1 gcttcgttga aggggaaccc atcgtgggcg gttgggacgc ccccgagcag gtcccttccg
       61 agacctccct gtcgcggctg gtgagcgggg aactgaaaag aaggggtttc actttcatgg
      121 gtcccgtcgt cgtctactcc cacctgcagg ccgtcgggct cgtcaacgac cacctgaggc
      181 gctgcttccg ctacggccga tcgtcctcct gaagcaggtg ctcttcctcg atggaaagat
      241 catccacccc cgcgagggac tctatgtggt gcctcagttc gtgggtcacc gtttccagga
      301 tctcctcctc ccattcttca agaggagcgc cccccatcgc ctcgcggaag gacccgtagt
      361 agaggattat gatattgccc agccccgggt cttctatata ctcgcccatg atgaggtagc
      421 cgccctcttc cttgatcggg gtctccacga ttatcccgcc gttgagccgt tccatcaatt
      481 ccccgggaac ggtctcaacg gcttcgcgga cggaccgcct gaactgtttc aggctcatga
      541 tcgccattgg tggccctcct ttaagggtcc cttcgggtgt tccttacggc tcccggccgc
      601 gaggtagtaa aatgtattat gtatgtgaaa tattgtgcta aaataagtaa cagtaaatat
      661 cattatcaca gggaggtagt ttttagatga aattctccaa gagagtaacc tcgatgcaat
      721 cttcgcccat ccgcaaactg gtgccctacg ccgaggcagc caaggcggag ggacggaagg
      781 tgtatcatct caacatcggg cagcccgaca ttgtaacccc cccgggtttc cttgagtcca
      841 tcaagagttt cgccgaggac gtcatcgcct actcaacctc ccatggcagc tacgagttga
      901 tagacgcgat gatagggtac tacaagggtt acgacatcca cctcgagagg aagaacctca
      961 tcgtcaccaa cgggggcagc gaagctctct ctttcgctct catggccatc tgtgaccccg
     1021 gcgacgaggt gctcgtcccc gagccgttct acgctaatta caacgccttc gcctcttccg
     1081 tcaacgtgaa actcgtcccc atcacgacca aggccaggga aggcttccac ttccccccca
     1141 aggccgacat cctgaagaag atcacccccc tgacgagggg gatcctcttc tcgaaccccg
     1201 gcaaccccac gggcgtcatc tacacgaagg aagagatgga gatgatcgcc gacatagcga
     1261 tggagaagga cctctacgtc atctccgacg aagtctaccg ggagttcgtc tacgacggcc
     1321 tgaagtacac cagcttcggc aacatgaaga ggatagagga cagggtcatc ctcgtcgact
     1381 ccatctccaa gcggtacagc gcctgcggcg ccaggatagg ctgtatcatc tccaagaacg
     1441 acgaactcat ggcccagacc atgaaactgg ctcagggccg gctctgcgtg cccaccctgg
     1501 agcaggtggg ggccgtggcc ctctacaaaa cgccccggtc ctacctggag gaggtcaaca
     1561 aggagtacca ggcccgcagg gacatactct actccaagat caaggagatg cccggggtga
     1621 tctgcgagga gcccaagggc gccttctatg tggtggccaa gctccccgtc gacaacgccg
     1681 agaaattcgt catctggatg ctgaaggact tccacgtcga taacgagacc gtcatgatgg
     1741 ccccggccga gggcttctac gccacgccca acctgggcag ggatgaggtc cgcctggcct
     1801 atgtcctcaa gaaggaggac ctgaccaggg ccatggacat cctcaaagcc ggcctcgagg
     1861 cctacccggg gaggatcaag accgtctcca acttctagtc ccgaacggcc ccgggtccgc
     1921 aggaaccgaa gccgcccctc ctcggggcgg cttttttgat cggcttcggc gcggccgcgt
     1981 cactcccggc gcctcaggac ccttacgaca cccctgacca cgccccaggt ggaggcgccg
     2041 cccgaaggga cctcccagag cgcgagcttg ccgtccctgg ttatgacggc caggtcgccc
     2101 ttttcagcct ggcaggagcg gtccacgacg aggacgtcgc cttcttctat ggaccaggcg
     2161 gggatcctcc ccgccga
//
LOCUS       DAOSRG010000207         2137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_47153_length_2137_cov_8.805956, whole genome shotgun sequence.
ACCESSION   DAOSRG010000207 DAOSRG010000000
VERSION     DAOSRG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47153_length_2137_cov_8.805956"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLM30_06205"
     CDS             complement(<1..364)
                     /locus_tag="PLM30_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOR54798.1"
                     /translation="MDNRSMVTRFLDVIEGDILPLTERAVPRGHNIFGAAVLRAGDLS
                     LVVAATNERSADPTLHGEMAAIRAFFRGKGHPDPADTVFLATHEPCSMCLSALAWSGF
                     RKIYFLFTYEETRDDFRMG"
     gene            439..1782
                     /locus_tag="PLM30_06210"
     CDS             439..1782
                     /locus_tag="PLM30_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOR54799.1"
                     /translation="MKTLPAPAGKRRSPFSAAALLLLLLLLLPFPAAALEAPRTAFRF
                     VGKDFGAPMIDEAAQKEMADLFRKNYFSPWDRSAPEVEPGESLWAWERWKDGEPWGEN
                     LRPHSKEWVRSLLALCDLDRWGSVSRKAVACVDTDLRALPTSRSFFEDPSRAGEGYPF
                     DYLQNSRVKAGEPLFVGHYSADGAWAFVETGYASGWVDSRDIALVDEDFAQRWRSLPQ
                     AFVIDDGVTISDRGGVYRFTARVGTVLPVVGGGKGGPLVIAVPVRDERGRAVRVEALL
                     SPGQVSDGPLPLTEWNLAMVIDRMIGKSYGWGDLGGNRDCSGSLRDLFTAFGVWLPRN
                     SAAQARAYPFVPFGEVPGREKEERLALEGVPFATLLAVPGHIMLYLGTYRGEPWVFHS
                     MWGVRTLRDGKEGRHIVGSAVITTLRPGRGVADIDPRRGDLAERLLGMTFLVPSR"
     gene            complement(1779..>2137)
                     /locus_tag="PLM30_06215"
     CDS             complement(1779..>2137)
                     /locus_tag="PLM30_06215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR54800.1"
                     /translation="DMTGFKEVNDTYGHLMGDAALQAASLCVRDSLRESDFVARFGGD
                     EFVVLMPETGVEEAAAATSRISEKLVKVRVSGIPLKLTAHFGLSRYPEDGKKLAAVLK
                     KADDRMCEEKTRPGNN"
BASE COUNT          344 a          687 c          716 g          390 t
ORIGIN      
        1 cgcccatcct gaagtcgtcg cgggtctcct cgtaggtgaa gaggaagtag atctttcgga
       61 accccgacca ggcgagggcg gaaaggcaca tggaacaggg ctcatgggtg gccaaaaaga
      121 cagtgtcggc ggggtcgggg tgccccttgc ccctgaaaaa ggcccggata gccgccattt
      181 ccccgtgaag ggtggggtcg gcgctccttt cgttcgtggc cgcgaccacc agggataggt
      241 cgccggcccg aagcacggcg gcaccgaaaa tgttgtgccc cctaggcacc gccctttcgg
      301 tgaggggcag aatgtccccc tctatcacgt cgaggaacct cgtcaccatg ctccggttgt
      361 ccaagccgtc accccccttc aaagatatca gaaaggtcgt caaaggggga aacctccttc
      421 gagggagtgg tgggacaaat gaagaccctt ccagctccgg ccggcaagcg ccggtccccg
      481 ttctccgcgg cggccctcct cctgttgctg ctgctattgc ttccctttcc cgcggcggcc
      541 ctcgaggcgc cgaggacggc cttccgcttc gtggggaagg atttcggggc ccccatgatc
      601 gacgaggccg cgcaaaaaga gatggcggac cttttcagga aaaactactt ctcgccctgg
      661 gacaggagcg cccccgaggt cgagcccggt gaatccctgt gggcctggga acggtggaag
      721 gatggcgaac cttggggaga gaacctcagg ccccactcga aggagtgggt gcgttccctc
      781 cttgccctat gcgacctgga caggtggggc tcggtgagcc ggaaggccgt ggcctgtgtc
      841 gacaccgacc tcagggccct gccgacctcg aggtccttct tcgaggaccc ttcccgcgca
      901 ggagaaggtt accccttcga ctacctccag aattccaggg tgaaggctgg cgagcccctc
      961 ttcgtggggc attactccgc cgacggggcc tgggctttcg tcgagacggg ttacgcctcg
     1021 gggtgggtcg actcgaggga tatcgccctg gtggatgaag acttcgccca gcgatggcgg
     1081 tccctgccgc aggccttcgt catcgacgac ggcgtcacca tcagcgaccg cggcggcgtc
     1141 taccgcttca ccgcccgggt gggcacggtg ctccccgtag tggggggcgg caagggcgga
     1201 cccctggtca ttgcggtgcc cgtgagggat gagcggggcc gagccgtccg cgtcgaggcc
     1261 ctcctttcac cggggcaggt ctccgacggg cctttgccgc tgacggagtg gaacctggcg
     1321 atggtcatcg acaggatgat cggcaaatcc tacggctggg gcgacctggg ggggaaccgg
     1381 gactgctcgg ggagcctgag ggacctcttc accgccttcg gcgtctggct cccccgtaac
     1441 tcggcggccc aggctcgggc ttaccccttc gtccccttcg gcgaggtccc gggccgggaa
     1501 aaggaggaga ggctggccct ggaaggggtg cccttcgcga ccctgctggc ggtgcccggg
     1561 catatcatgc tctacctggg gacctaccgt ggtgagccct gggtatttca ctcgatgtgg
     1621 ggcgtgagga cgctccgtga cgggaaggaa gggcgccata tcgtgggctc ggcggtcata
     1681 acgacgctcc ggcccggcag gggcgtggcg gatattgacc cccggcgggg cgacctggcc
     1741 gaaaggctcc tggggatgac ctttctcgta ccctcccgct agttgttccc agggcgcgtt
     1801 ttttcctcgc acatcctgtc gtcggccttt ttcaggacgg cggccagttt tttcccgtcc
     1861 tcggggtaac gggaaaggcc gaaatgggcc gtcaacttca gcggtatgcc gctgactcgg
     1921 actttcacca gcttttccga gatcctgctc gtcgccgcgg cggcctcttc cacccccgtc
     1981 tcgggcatca ggacgacgaa ctcgtcgccg ccgaagcggg ccacgaagtc cgactccctg
     2041 aggctgtccc tgacgcaaag cgacgcggcc tgaagggcgg catcgcccat caggtgcccg
     2101 taggtgtcgt tgacctcctt gaaaccggtc atatcca
//
LOCUS       DAOSRG010000208         2127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_47449_length_2127_cov_11.593629, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000208 DAOSRG010000000
VERSION     DAOSRG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47449_length_2127_cov_11.593629"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="recA"
                     /locus_tag="PLM30_06220"
     CDS             complement(<1..1004)
                     /gene="recA"
                     /locus_tag="PLM30_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOR54801.1"
                     /translation="MGKRKGSMTREDVLDQALSEIRSKFGEGAIMCLGESSGAPAEVI
                     STGVLPLDIALGIGGYPRGRIVEIFGPEGGGKTTLALHALAEAQKAGGIAAFIDAEHA
                     LDPRLAFSLGVRIEDLYVAQPDSGEQALYILETLVRSGAVDVIVVDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISRSNCVVIFINQLRAKISTMGYGGPQETTTGG
                     RALKFYSSVRIEVKRGKSISRGDENLGHELWIKVVKNKQAPPFRTAHASLIYGKGIPA
                     EISVLDMALDYDILKRKGSWIAYKGESLGQGKESVGNYLMEHQDLMEEIIAAVKAKAT
                     "
     gene            complement(1007..1603)
                     /gene="thpR"
                     /locus_tag="PLM30_06225"
     CDS             complement(1007..1603)
                     /gene="thpR"
                     /locus_tag="PLM30_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOR54802.1"
                     /translation="MPGDLVRCFVCVQLGGAVKEKLSRWVSPFRKMMPELRWVKEDAY
                     HITLKFCGEIPYTLLYKLESALEHGLSLKKTRPFPLELAGIGAFPGFRQPRVLWAGIG
                     GEDYLVQRLAAVAERAAVAAGIEREKRPFHPHVTLARIPPGADLPVGALREMNSSSES
                     WGEWTVSSLTLMRSDLLPEGPRYSPIAVFEFSNDLEVQ"
     gene            complement(1593..>2127)
                     /locus_tag="PLM30_06230"
     CDS             complement(1593..>2127)
                     /locus_tag="PLM30_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOR54803.1"
                     /translation="PIMKEKKEKADLVDDLSRKVLAEARAGGLTISCAESCTGGLLAG
                     AITDIAGSSDVFAGGVISYSNRSKSMVLKVPEELIREHGAVSEECALAMARGAMDLFE
                     SDVACSVTGIAGPGGATPGKPVGLVWFALVSRSGGKTFAEFFKGNRRAIREMAVIFAL
                     TEMVAALKRARGVGHAG"
BASE COUNT          417 a          665 c          572 g          473 t
ORIGIN      
        1 tccgtggcct tggccttgac agcggctatg atctcttcca tcaggtcctg gtgttccatg
       61 agatagttcc ccacgctctc cttgccttgg ccgaggcttt cccccttgta ggctatccag
      121 gaaccttttc tcttgagaat atcgtaatcg agagccatgt ccaggaccga tatctcggcg
      181 gggatcccct tgccatagat caaggaagcg tgagcggtcc tgaagggcgg ggcctgcttg
      241 ttcttgacga ccttgatcca gagttcatga cccaggtttt catcgcccct ggagatggac
      301 ttgcccctct tgacttcgat ccttacggaa ctgtagaact ttagggcccg cccgccggtg
      361 gttgtttcct gcggcccacc gtaacccatg gtggagatct tggccctgag ctggttgatg
      421 aatatgacca cgcagttgct cctggagatg gccgaagtga gcctcctcag tgcgtaggac
      481 atgagccttg cctggaggcc gacctggctg tcgccgatct tgccgtcgat ctctgcctgg
      541 ggcgtcaggg cggcgacgga gtcgactacg atcacatcga cggctccgct cctgaccagg
      601 gtttccagta tatagagggc ctgctcgccg ctgtcgggct gggccacgta aagatcctcg
      661 atcctgacgc cgagcgaaaa ggccaaacgg gggtccaagg catgctcggc atcgatgaaa
      721 gcggctatac ccccagcttt ttgggcctcc gcgagggcat gaagcgccag tgtcgtcttg
      781 cctccgcctt caggtccgaa gatctccact atccttcccc tggggtatcc tcctatgcca
      841 agtgcgatgt ctagggggag cactcccgtg gagatcactt ctgcaggtgc cccggagctt
      901 tcccccaggc acatgatcgc cccttccccg aatttactcc gtatttcgct caatgcctga
      961 tcaagcacgt cttctctggt catggagccc ttccgtttcc ccatggtcac tgcacctcca
     1021 ggtcatttga gaattcaaaa accgcaatgg gagagtacct gggtccttcc ggcaaaagat
     1081 cgctcctcat cagggtcagg gaagacacgg tccattcacc ccaggactcg ctgctgctat
     1141 tcatctctct caaggccccc acgggaagat ccgctcccgg gggtatcctg gccaaggtga
     1201 catgagggtg aaagggcctc ttttctcttt cgataccagc ggcgacggca gccctttcgg
     1261 cgacggctgc caacctctgc accaagtagt cctcgccccc gatcccggcc caaagaaccc
     1321 tgggttgacg aaaaccgggg aaggcgccga tccctgccag ttccaggggg aagggccttg
     1381 tctttttaag cgacaagccg tgttccaggg cactttcaag cttgtaaaga agagtataag
     1441 gtatctcccc gcagaatttg agcgttatat ggtaggcatc ttctttgacc caccgcagtt
     1501 ccggcatcat tttcctaaag ggggaaaccc acctggacag cttttccttg accgcgcctc
     1561 ctagttggac gcagacgaaa cacctaacaa ggtcacccgg catgaccgac acctctcgca
     1621 cgcttcaatg ccgcgaccat ttctgtgaga gcaaagatga cggccatttc acggatcgct
     1681 ctcctgtttc ccttgaagaa ttcagcgaag gtctttccac cggagcgcga aaccagggcg
     1741 aaccagacca acccgacggg tttccccggt gtcgcccctc ccggacccgc gatccccgtg
     1801 accgaacagg cgacgtcgga ttcgaaaaga tccatagccc cgcgggccat cgccagggcg
     1861 cactcttcgc tgacagcccc gtgctctcgg atcaactcct ccgggacctt caggaccatg
     1921 gacttggaac ggttcgaata actgatgacc cctccagcga agacgtcaga ggaacctgcg
     1981 atatcggtga tggcccccgc caggagacca ccggtgcatg attcggcgca ggagatggtc
     2041 aatccgccgg ctctcgcttc agcgagtacc ttgcgtgaaa ggtcatcaac gaggtcggcc
     2101 ttttcttttt tttctttcat gatcggg
//
LOCUS       DAOSRG010000209         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_47577_length_2123_cov_9.019826, whole genome shotgun sequence.
ACCESSION   DAOSRG010000209 DAOSRG010000000
VERSION     DAOSRG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47577_length_2123_cov_9.019826"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            562..>2123
                     /locus_tag="PLM30_06235"
     CDS             562..>2123
                     /locus_tag="PLM30_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOR54804.1"
                     /translation="MFGKLLHVDLGTGAFGDHVVPEEAVRKYIGGKGLGIYLLDSMTA
                     PGTGALDPGNALFFLTGPFTGTIFPTSGRFVVVTKSPLTGMYVDSHSGGHFAKELRRA
                     GYDGIIVTGKAERPVFLWIEDGRAELRDASGLWGKTVDETVSAVRGMTDDKARVAVIG
                     PAGENLVPLATITIDSDSDQTRAGVAGRGGTGAVMGSKNLKAIAVRGTGAITVAEEGP
                     FREQVAEIVKAINANDQVHTRRIVGTSSLVESMSRTGILPTYNFQQGYFVPIYGIVST
                     NMRHYTKRDVACSNCPIICGKVLDDEGHAVKMEYESIALLGSNNGVGDMPSLARSVRV
                     CNQLGLDTISAGNVVGFAMECRQRGILPEAPEFGDAGGQAELLEDMAYRRGLGALLAD
                     GVKAAAERVGKGTDHFAIHVKGMELPGYEPRATWGMALAYATSDRGGCHQRAWTVLGE
                     LDGYLPRFSTEGMAATVKTIQDERAAAYSLVVCDFAPYEQERALACLRHVTGWDITEE
                     EYLAAGERTWNHT"
BASE COUNT          475 a          583 c          784 g          281 t
ORIGIN      
        1 cgcccgggtt tccccgggag gccccgtcga aataggctgt agtcatgggt atcccctcac
       61 ctcacccctg ttcatgaaaa agtataacag agccagggct ttggggtcca aggagcgtga
      121 gacggaagac ggaagaccaa aggcaaggcg acaggcggca ggcgacaggc aacaggaagg
      181 gcaaaatccg tgaatcggga atcgttaatc gggaatcgaa aaggcaaaga acagaaggcc
      241 aaggagcgtg agacgggaga cggaagacca aaggcaagga gacaggcgtc aggcgcaggc
      301 gacagaaggg gcaagagacc gaagaccaaa gggcgtaaga aggaaaggca aggcgccagg
      361 agacaggcaa caggaagggc aagggcgaaa agcaaaagag cgtaaaacgt gaaacgtaag
      421 acaggaagag cggaaagcaa ggccaagggc aaaaggccgg gacgagggcg aagggcgcgg
      481 ggctggggac gggtggtggg gggttgcggg tagaataggg gcgaaggctt cacttttccg
      541 atctcgcaag gggggagaaa gatgttcgga aaacttctgc acgtcgatct tgggacgggg
      601 gccttcgggg atcacgtcgt ccccgaggag gcggtgagaa agtacatcgg cgggaagggg
      661 ctcggtatct acctgctgga ttcgatgacg gcgccgggaa caggagcgct ggacccgggg
      721 aacgcccttt tcttcctgac agggcccttc actgggacga tattcccgac ctcaggccgt
      781 ttcgtggtgg tgaccaagtc gcccctcacc gggatgtacg tggacagcca cagcgggggg
      841 cacttcgcga aggagctgcg gcgggccggc tacgacggga tcatcgtgac cggcaaggcg
      901 gaaagaccgg tcttcctctg gatcgaggac ggcagggccg aactgcgcga cgcgtcgggc
      961 ctctggggga agaccgtgga cgagacggta tccgctgtca gggggatgac cgacgataag
     1021 gcccgcgtgg ccgtgatcgg gcccgcgggg gagaacctgg tgcccctggc caccatcacc
     1081 atcgacagcg acagcgacca gaccagggcg ggcgtggcgg gcaggggcgg caccggggcg
     1141 gtcatgggga gcaagaacct caaggccata gccgtcaggg gcaccggggc cataaccgtc
     1201 gccgaggagg gccctttcag ggagcaggtg gccgagatcg tcaaggccat caacgccaac
     1261 gaccaggtcc acaccaggcg catcgtgggg acctcctcgc tggtcgagtc catgagccgc
     1321 acgggaatcc tgcccaccta caatttccag cagggttact tcgtgcccat ctacgggatc
     1381 gtctccacca acatgaggca ctacacgaag agggacgtgg cctgctccaa ctgccccatc
     1441 atctgcggca aggtcctgga tgacgagggg cacgccgtaa agatggagta cgaatccatc
     1501 gcgctgctgg ggagcaacaa cggcgtgggc gatatgccgt cgctggcgcg gtcggtgagg
     1561 gtctgcaatc aactcggcct ggataccatc tcggcgggga acgtcgtggg cttcgccatg
     1621 gagtgcaggc agagggggat ccttcccgag gcgcccgagt tcggcgacgc cggggggcag
     1681 gcggaactgc tggaggacat ggcctacagg agaggcctgg gggcgctcct ggccgacggg
     1741 gtgaaggcgg ccgccgaaag ggtcgggaaa gggacggacc atttcgccat ccacgtcaag
     1801 gggatggagc tgcccggcta cgaacccagg gcgacctggg ggatggccct ggcctatgcc
     1861 acctccgacc ggggaggctg ccaccagagg gcctggacgg tgctgggcga actggacggg
     1921 tacctgcccc gcttctccac ggagggcatg gcggcgacgg tgaagaccat ccaggacgag
     1981 agggcggcgg cctattctct cgtggtctgc gacttcgccc cctacgaaca ggagagggcc
     2041 ctggcctgcc tcaggcacgt cacgggttgg gacatcaccg aggaggagta cctggccgcc
     2101 ggtgagagga cctggaacca cac
//
LOCUS       DAOSRG010000210         2122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_47609_length_2122_cov_9.447025, whole genome shotgun sequence.
ACCESSION   DAOSRG010000210 DAOSRG010000000
VERSION     DAOSRG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47609_length_2122_cov_9.447025"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLM30_06240"
     CDS             <1..256
                     /locus_tag="PLM30_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54805.1"
                     /translation="RALVSVPLHALCGLAMGYEAARQKMTAGPPFALAKILTIPVIAH
                     GAFNYLLLTNTLGGFMAATWIVAAMWIGGFRMIHRSRTCS"
     gene            273..1544
                     /locus_tag="PLM30_06245"
     CDS             273..1544
                     /locus_tag="PLM30_06245"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOR54806.1"
                     /translation="MKKPSMKDIFSRKGLLSQRFPGFEHRPQQEALAERVMASLDSGG
                     GGVLLAEAPTGTGKTHALLVPAVYWCLEDDRKILFLTSGIPLQEQIVKRDLPDLCDCL
                     GVEDLSYGLIKGKANYLCRLRGKEISERELLAHLEPDLVLELVAWMEKTETGDFSELA
                     LPPGHPVISALSAASDACRGNGCPFRESCFLRRVLQQAQEWNIIVANYHLFFSYSAST
                     GRGFPVPYDAILCDEAHRLTEAARSAASLRATGEEWARVMDFAKARVFPRMEWVSDHD
                     RQRLALLADDVKKESERLFHLWKKEVPAGRIFTDEAGPPDGPGPLQGLAGEVLEAAGE
                     PPEKNGADDRESAALLHQWRDSVADLSHRTAWCARVDRFPLWAYWWDGRSINSSPTFC
                     GDLISDWFFAKPLEALVAVSATMAIGEDFSF"
     gene            1614..>2122
                     /locus_tag="PLM30_06250"
     CDS             1614..>2122
                     /locus_tag="PLM30_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOR54807.1"
                     /translation="MSIWVVDLGLAVTDDGYDDLVARIAEQLCDDNDGSTLVLLSSLR
                     LLRAVSERLRSKEHPYRVLEQGELPRSELLRLFRQDLSSVLVGSVSFREGIDVPGEGL
                     TQVIIDRIPFPHPADPVLVTRKILEGPDMFFDIVLPRAKLLLKQAAGRLIRSKSDRGR
                     VVILDGRVLS"
BASE COUNT          358 a          757 c          661 g          346 t
ORIGIN      
        1 gagggccctc gtctcggtgc cccttcacgc cctctgcggc ctcgccatgg gttacgaggc
       61 ggcccgccag aagatgaccg ccgggccccc cttcgccctc gcgaagatac tcaccatccc
      121 cgtcatcgcc cacggggcgt tcaactacct gctgctgacg aacaccctcg gcgggttcat
      181 ggcggccacc tggatcgtag cggccatgtg gatcggcggt ttcaggatga tccaccggtc
      241 ccgcacctgc tcgtgacgga ggagccacgc ccttgaaaaa gccctccatg aaggacatct
      301 tttccaggaa aggcctgctc tcgcagcggt tccccggttt cgagcaccgg ccccagcagg
      361 aggccctggc ggaaagggtc atggccagcc tcgattccgg tgggggcggt gtcctgctgg
      421 ccgaggcccc taccgggacg ggcaagaccc atgccctcct cgtgccggcc gtctactggt
      481 gcctcgagga cgaccgaaaa attctcttcc tcacctcggg catccccctg caggaacaga
      541 tcgtaaaaag agacctcccc gacctctgtg actgcctggg cgtggaggac ctctcctacg
      601 gcctcatcaa gggaaaggcc aactacctct gcaggctccg gggcaaggag ataagcgaga
      661 gggaactgct ggcccacctg gagcctgacc tcgtccttga actcgtcgcc tggatggaaa
      721 agaccgagac gggcgacttc tccgaactgg ccctcccgcc gggtcacccc gtcatatccg
      781 ccctgtcggc cgcatcggat gcgtgccggg gcaacggctg ccccttcagg gagagctgct
      841 tcctccgcag ggtcctgcag caggcccagg agtggaacat tatcgtcgcc aactaccacc
      901 tcttcttctc ctactccgcc tccaccggga ggggattccc ggtgccctac gacgccatcc
      961 tctgcgacga ggcccaccgg ctcaccgagg ccgcccggtc ggccgcctcg ctgcgggcca
     1021 ccggtgagga gtgggccaga gtcatggact tcgccaaggc ccgcgtcttt cccaggatgg
     1081 aatgggtctc cgaccacgac cgccagcggc tggccctcct ggccgacgat gtgaaaaagg
     1141 agtccgaaag gctcttccac ctctggaaga aggaggtccc cgcgggccgg atattcaccg
     1201 acgaagccgg cccccctgac ggtcccggcc cgctccaggg cctcgccggg gaggtcctcg
     1261 aggcagccgg tgagccgccc gagaagaacg gcgccgacga cagggagtcg gcggccctcc
     1321 ttcaccagtg gagggacagc gtggcggacc tgtcgcaccg caccgcctgg tgcgccaggg
     1381 tggaccgctt cccgctctgg gcctactggt gggacggccg gagcatcaac agctcgccta
     1441 ccttctgcgg cgacctcata agcgactggt tcttcgccaa gcccctggag gccctggtgg
     1501 cggtgtcggc gaccatggcc ataggagagg acttttcctt ctaggagcag gagacgggac
     1561 tgcaggccac cgagaggctt gtcctcgact cgcccttcga cctggacgag cagatgtcca
     1621 tctgggtcgt cgacctgggc ctcgccgtga ccgacgacgg ctacgacgac ctcgtcgcca
     1681 ggatcgccga acagctctgc gacgacaacg acgggagcac cctggtcctg ctgagctccc
     1741 tgaggctcct ccgggccgtt tctgagcggc tgaggtcgaa ggagcacccc taccgcgtcc
     1801 tggagcaggg ggaactcccc cgtagcgaac tgctgaggct cttccgccag gacctctcct
     1861 cagtcctcgt ggggagcgtc tccttccggg agggcatcga cgtccccggc gaggggctga
     1921 cccaggtcat catcgaccgg atacccttcc cgcaccccgc cgaccccgtg ctggtcacca
     1981 ggaagatcct cgagggcccc gacatgttct tcgacatcgt cctcccccgg gccaaactgc
     2041 tgctgaagca ggccgccggc aggctcatca ggagcaaaag cgaccgcggc agggtggtga
     2101 tcctggacgg gcgcgtcctc tc
//
LOCUS       DAOSRG010000211         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_48916_length_2082_cov_7.594475, whole genome shotgun sequence.
ACCESSION   DAOSRG010000211 DAOSRG010000000
VERSION     DAOSRG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48916_length_2082_cov_7.594475"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLM30_06255"
     CDS             <1..293
                     /locus_tag="PLM30_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HOR54808.1"
                     /translation="SREAQKYGYELLLPGPDIQEELQEAVALVKANQLTRSGAAMAEA
                     VGRLKEQRDLPVIGACTELPLAYEASGHDPGGMVSSLGSLAEACLERIYSPS"
     gene            complement(278..1702)
                     /locus_tag="PLM30_06260"
     CDS             complement(278..1702)
                     /locus_tag="PLM30_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOR54809.1"
                     /translation="MRRWFSNVAREELVLGYAFVKWAVLSVAAGVVVGAAAAVFLLLL
                     GKGIGAAGLLGRFRVLLLPFGLAASVFTVRTLAPEAAGHGTEKVIEAVHQRSGRISLP
                     TVPVKLAATIITIASGGSAGKEGPCAQIGAGLTSAMAGLLRLNDADRKKLVVCGISAG
                     FSAIFGTPVSGAIFGLEVLYIGQVYYDVMLPAFISGIVAFQTAAHLGVHYADQSLTAF
                     PALASSSLAWVALSGLFFGLVALIHIEIVSVTERYLKGITARPEVKAFGSGVALLLLS
                     LLLGTRYFGLGTEFIEAAVSGEPVQGGAFLVKSFFTGVTLAAGGTGGILTPTFFVGAG
                     AGSFFASLFHLDLSLFSSIGFVAVLAGAANTPIAATIMATELFGGGIASFAAVACIIS
                     FAISGHRSIYPSQILARPKSRVFQFSGEKVAVDKHSHKINLSETIIPTVVSWLRGNWE
                     SLTRLSRGGKKRSTPKDPPGQDGE"
     gene            complement(1825..>2082)
                     /locus_tag="PLM30_06265"
     CDS             complement(1825..>2082)
                     /locus_tag="PLM30_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54810.1"
                     /translation="GSGTGSAPCIKRLVSAGLRVEQVPEEYSSERGRAIYWQIHPPRG
                     LQRLIPRGLLVPPRSVDDLAAWSLVLRRVGRDDPARIRRKD"
BASE COUNT          391 a          685 c          682 g          324 t
ORIGIN      
        1 acagccgcga ggcccagaag tatggttacg aactgctcct ccccggcccg gacattcagg
       61 aggagctgca ggaggcagtg gccctggtca aggccaacca gttgacccgt tcgggggcgg
      121 ccatggcgga agccgtcggc cgcctcaagg agcaaaggga cctccccgtg atcggggcct
      181 gcaccgagct gcccctcgct tacgaggcct cggggcacga ccccggcggg atggtctcca
      241 gcctcggatc cctcgccgaa gcctgcctgg aaaggatcta ctccccgtcc tgaccgggag
      301 ggtccttcgg ggtagaccgc ttcttcccgc ccctggagag gcgggtcaag ctttcccagt
      361 tccccctgag ccaggagacg acggtgggga tgatggtctc tgaaaggttg atcttgtggc
      421 tgtgcttgtc gacggccacc ttctccccag aaaattggaa aacccgggat ttgggccggg
      481 ccaagatctg gcttgggtag atgctcctgt gaccgctgat ggcgaaggag atgatgcatg
      541 ccaccgccgc gaaggaagct atccctccgc cgaaaagttc cgtggccatg atcgtggcgg
      601 cgatgggggt gttggccgcg ccggccagga ccgccacgaa gcctatggag ctgaacaggg
      661 agaggtcgag gtgaaagagg gaggcgaaaa aggagccggc gccggcgccg acgaaaaagg
      721 tcggcgtcag gatgccgccc gtccccccgg cggcgagggt gacgcccgtg aagaaggatt
      781 tgaccaggaa ggctcccccc tggaccggct cccctgaaac ggcggcctcg atgaattcag
      841 tccccaggcc gaagtaacgg gtacccaaca gcagcgagag caacaacagc gccacgccgc
      901 tcccgaaggc cttgacttcg ggcctcgccg taattccctt gagatacctc tccgtgaccg
      961 atacgatctc gatgtggatc agggccacga ggccgaagaa gagtcccgac aaggccaccc
     1021 aggcgaggct cgagctcgcc agggccggga aggccgtcag gctctggtcg gcgtagtgga
     1081 cccccaggtg ggcggccgtc tggaaggcca cgataccgct gatgaaggcg gggagcatca
     1141 cgtcgtaata gacctggcct atgtagagga cctcgagccc gaagatggcc cccgatacgg
     1201 gtgttccgaa gatggccgaa aaccccgccg agataccgca gaccaccagt tttttccggt
     1261 ccgcgtcgtt aagcctcagc aggccggcca tcgcggaggt caggcccgcg cctatctggg
     1321 cgcagggccc ttctttgccc gccgacccac ccgaagcgat ggtgattatc gtggcggcca
     1381 gtttcacggg gaccgtgggc aatgaaatgc gccccgaccg ctggtggaca gcctcgatca
     1441 ccttctcggt cccgtggccg gccgcctccg gcgccagcgt cctgacggtg aagacgctgg
     1501 ccgcgaggcc gaagggcaag agcagcaccc tgaaacggcc caggaggccg gccgccccga
     1561 tcccttttcc aaggagaagg aggaaaacgg ccgcggccgc ccccaccacg acccccgcag
     1621 cgacggagag caccgcccac ttcacgaagg cgtacccgag gaccaattcc tccctggcta
     1681 cattgctgaa ccagcgcctc atgacctctc ggcgcccatc ccctctctca tgacccgatc
     1741 aacctctctt tctcacagga cttacagaca cgcggcaccg gggcgggcgt cgcgttttgg
     1801 atgacccccg gcgatggtgc gggttcagtc ttttctccgg atccgggcgg ggtcgtcgcg
     1861 gccgacgcgg cggaggacaa ggctccacgc agcgaggtcg tcaacgctcc tgggggggac
     1921 gagaaggccc ctggggatga gccgctgtaa tcccctgggg gggtggatct gccagtagat
     1981 cgccctcccc ctttccgatg aatattcttc ggggacctgt tcaacccgga gccccgcgga
     2041 gaccagcctc ttgatgcagg gggcgcttcc cgtgccggaa cc
//
LOCUS       DAOSRG010000212         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_49267_length_2072_cov_4.507685, whole genome shotgun sequence.
ACCESSION   DAOSRG010000212 DAOSRG010000000
VERSION     DAOSRG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49267_length_2072_cov_4.507685"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLM30_06270"
     CDS             complement(<1..784)
                     /locus_tag="PLM30_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR54811.1"
                     /translation="MTPVVKIARWLKADVRRYRLVVWSVMALVYMVVFFHRLAPGVVR
                     DDLVAVFGMSGAAFGHLASMYFYAYTAMQIPVGMLADSLGARKTVTTGIFLSGAGSLA
                     FGLAPSPALVYLGRFVVGVGASAVFVCILKMLSDWYSEREFATMSGVTAFVGNSGGLV
                     AQAPLAMMVACFSWRMTFVAIGGFSFLLGAVSLLLIRDRPSDMGFAPVNPTGAGPKIG
                     GGELLHAVGEALRSRGIWPAMTIAGLFCGGQLAFMGAWGVPWL"
     gene            1532..>2072
                     /locus_tag="PLM30_06275"
     CDS             1532..>2072
                     /locus_tag="PLM30_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54812.1"
                     /translation="MAITVTGYHRAGEIEEELARLGSGRDPSPVFLVPSTGDRELLRD
                     IILEKVSFGPSEPPILRWEDLYREAAREMDVPRGARRRQIDPPDHWLIVRHVLERLKA
                     RVEEAAIPAGARHGGFIWTLGEDLRELLREEVRPGILAQSMGCDQRCQGSDCPKLPDP
                     GALLCRLYRDYLEYLDAHSL"
BASE COUNT          417 a          660 c          677 g          318 t
ORIGIN      
        1 tcagccatgg gaccccccag gcgcccatga aggccaactg tccgccgcag aaaagtcccg
       61 cgatggtcat cgcgggccag atgcccctgg accggagcgc ctctcccacc gcgtggagca
      121 gttccccgcc gccgatcttc ggccccgccc cggtggggtt gaccggggcg aaccccatat
      181 cagaggggcg gtctcttatc aaaaggaggg agaccgcgcc caggaggaag gaaaatcccc
      241 ctatcgcgac gaaggtcatc ctccaggaaa aacaagccac catcatggcg aggggcgcct
      301 gggcgacgag cccgccggaa ttgcccacga aggccgtgac gccggacatg gtggcgaact
      361 ccctctcgct gtaccaatcg gataacattt tcaggataca gacgaatacc gcggaggccc
      421 ccacgccgac gacgaatcgc cccaggtaga ccagtgccgg ggacggagcc aggccgaagg
      481 ccagagaccc ggcccccgaa aggaagatac ccgtcgtgac ggttttcctg gcccccagcg
      541 agtcggccag catacccacc gggatctgca tggccgtgta ggcatagaaa tacatggaag
      601 ccaggtggcc gaaggccgcg cccgacatcc cgaatacggc gaccaggtcg tcccggacca
      661 ctcccggggc cagcctgtgg aagaaaacta ccatgtagac cagggccatg acgctccaga
      721 cgacgaggcg gtaccggcgg acgtcggcct tgagccagcg ggcgatcttg acgacgggtg
      781 tcatcgggga agctccttcc ggtggtatgt agtatcccca agggtgaccc ttggcaggcc
      841 ataagcatac tatatttttc gcagagcggg gtcaggtcta cacttttgac taaaaccagg
      901 ctttgccccg ctggggctgt ccggttacaa tgaggcaact caggccattg acaaatgccc
      961 cggggcgtta tagtatgctc caaacgtcag gaacggccca ctctttaaaa ggagggaaac
     1021 cgcgtgaagg acagggtcca gggctccgca gcagtgaccg gcaatgtcgc cattatcgtc
     1081 ctcgttctta cgtgggggtc ccctcctggg gcggccgtcg cgctgtaggc gcgtaaccgc
     1141 agaccgggac cggggccccc ttgcacgagg gggccccggt tttttattgg gttcgcggca
     1201 ctcctggagg atcgaccgga aggggagaaa agagatgcaa aacacgagag ggtttttttt
     1261 gaaccaggca ccggcgacgg ggcgaggcaa ggcaccggcg acggcagaac aagggaaccg
     1321 tcatagccgg gatcagtatc cccaggacct ttacgacccc aggtggagag ggcccttcga
     1381 aggatacagg tagagatgtc gcgaaccgcg ggaagggcgg gcgacccgcg accccggtcc
     1441 cggaggttcc caccagccat cgaccgcctg agcctcccca tttcgcgccg ggcacccgtt
     1501 ctgctatcat tgggcgggag gtagggtcgg catggcgatc actgtcaccg ggtatcaccg
     1561 ggccggagag atcgaggagg aactggcccg gctgggaagc gggcgtgacc cgtcccctgt
     1621 cttccttgtt ccttccacgg gggacaggga actcctccgc gacatcatcc tcgaaaaggt
     1681 ctccttcgga ccttccgagc cgcccatcct ccgctgggaa gacctttacc gggaggcggc
     1741 gcgggagatg gacgtgcccc ggggggcgag gcgaaggcag atagacccgc ccgaccactg
     1801 gcttatcgtc cggcatgtcc tggaacgcct caaggcaagg gtcgaggagg cggccatccc
     1861 cgccggggcg aggcacggcg gcttcatctg gaccctcggc gaggacctcc gggaactgct
     1921 cagggaagag gtccggcccg gtatcctggc ccagtccatg gggtgcgacc agcggtgcca
     1981 ggggtcggac tgcccgaagc tccccgaccc gggagcgctt ctttgccggc tttaccggga
     2041 ctacctggaa tacctggacg cccactccct gg
//
LOCUS       DAOSRG010000213         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_49354_length_2069_cov_6.098808, whole genome shotgun sequence.
ACCESSION   DAOSRG010000213 DAOSRG010000000
VERSION     DAOSRG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49354_length_2069_cov_6.098808"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLM30_06280"
     CDS             <1..648
                     /locus_tag="PLM30_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HOR54813.1"
                     /translation="FGGLNPHSVPYLIKKVKEHIPDKPIGVHFHDDFGLGAATTIMGL
                     ASGADIAHTSISGIGERAGNAPYEDVAMALLTTYGVDTGLRYEKIYPLSKYLREITGL
                     TVRQNRGIVGDYVADIESGIVAAWYKNVEGIAPLELSPFLYDLVGHPDTKVVIGKLSG
                     LPTVDIYLDKLGIETDDKDAKAEILKRIKDTALEKRRLLTIEEFGEIASSVLDRS"
     gene            950..1339
                     /locus_tag="PLM30_06285"
     CDS             950..1339
                     /locus_tag="PLM30_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOR54814.1"
                     /translation="MKREIRTDRSPDPIGPYSQGLVVGGRIYVSGQGPLDPATGKTPG
                     TIEEQTRQVLTNIRNILEAGGATMEHVVKTTVHLADLKDFQAFNGVYKEFFKPPFPVR
                     TTVGSALLGILVEIDVIAEVPGIGLSL"
     gene            1409..>2069
                     /locus_tag="PLM30_06290"
     CDS             1409..>2069
                     /locus_tag="PLM30_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR54815.1"
                     /translation="MVGTLMIGGKTVERILLADDDTGLCELLTEFLESEGFKVESVHD
                     GSKVVARVLAERFSLLILDVMLPGLSGMEVLKEIRRQSAIPVLMLTARGDDVDRIVGL
                     ELGADDYLPKPFNPRELVARIRAVLRRGRLDGSRAPQAEVYRVGDMVIDPAARHVSSG
                     GTRIELTTVEFDLLETLAQSSGVALSREALVKSVLKRSFSPFDRSLDVHISNLRRKIG
                     TY"
BASE COUNT          411 a          622 c          655 g          381 t
ORIGIN      
        1 ttcggcgggc tcaaccccca ctcggtcccc taccttatca agaaagtcaa ggagcatata
       61 cccgacaagc ccatcggtgt ccatttccac gacgacttcg gcctgggcgc ggccacgacg
      121 atcatgggtc tcgcctcggg cgcggacatc gcccacacct ccatatcggg catcggcgag
      181 agggccggca acgcgcccta tgaagatgtg gccatggccc ttctgacgac gtacggtgtc
      241 gatacgggcc tgcgctacga gaagatctat cccctctcga aatacctccg ggagatcacg
      301 gggcttacgg tgcgccagaa caggggcatc gtaggggatt acgtggccga tatcgagtcg
      361 gggatcgtgg cggcctggta caagaacgtg gagggtatag cgccccttga actctcgcct
      421 ttcctctatg accttgtggg ccaccccgac acgaaggtgg tcatcgggaa gctgagcggg
      481 ctccccacgg ttgatatcta cctggacaag ctgggcatcg agaccgatga caaggacgcc
      541 aaggcggaga tcctgaagag gatcaaggac acggcgctgg agaagaggcg cctgctgacc
      601 atcgaggagt tcggggagat cgcctcatcg gtcctggaca ggtcgtaagg taggacttcc
      661 cccttgggcc acggcccgat ctccctggaa agagcctata cttgggcgtg aagggcctcc
      721 tcgtggaggc tcttctacag gggttgcggt tacgatgacg agtagctggc cggctcctga
      781 tcggcattgt caacaccgcc aacggcgccg cggactcttc tttaagcgat gctggacgac
      841 agggcaagga cccaaaggcg taaacagtag gacccaggtg tttgtatggg aaattccgtt
      901 catcgtccgc catttccgct caagatcctt tttgggggag gtttttctga tgaaaagaga
      961 gatccgcacc gaccggtcac ccgacccgat agggccctat tcccagggcc tggtggtggg
     1021 cggcaggatc tacgtctcgg ggcaggggcc gctggacccc gccaccggca agacccccgg
     1081 gaccatcgag gaacagaccc ggcaggtcct gaccaacatc aggaatatcc tcgaagccgg
     1141 cggggccacc atggaacacg tggtcaagac caccgtccac ctcgccgatc tcaaggattt
     1201 ccaggccttc aacggcgttt acaaggaatt tttcaaaccc ccctttccgg tgaggaccac
     1261 cgtgggaagc gcgctgctgg gcatcctcgt ggagatcgat gtcatcgcgg aggtgccggg
     1321 gatcgggctt tcgctgtaaa tgagcaggag ggacttttta cgaaagggtt aaccttcttg
     1381 gcggccctta aggcccccat ggagtagtat ggtggggacc ttgatgatcg gggggaaaac
     1441 cgtggagcgg atactgctcg ccgatgacga cacgggcctt tgcgaattgc tcaccgagtt
     1501 ccttgaatcg gaaggcttca aggtcgagtc cgtccacgac gggagcaaag ttgtcgcgag
     1561 ggtcctggcg gaacggttct cgctgctgat actcgatgtg atgctccccg ggctctcggg
     1621 catggaggtc ctcaaggaga tccggcggca gtcggccatc cccgtcctga tgctcaccgc
     1681 ccggggcgac gacgtggacc gcatcgtggg cctcgaactg ggggccgatg attacctgcc
     1741 caagcccttc aaccccaggg aactggtggc caggatccgg gcggtgctgc gccggggacg
     1801 cctcgatggt tcccgggccc cccaggcgga ggtctaccgc gtaggcgaca tggtcataga
     1861 ccccgccgcg aggcatgtga gctccggcgg cacgcgcata gaactgacca cggtggagtt
     1921 cgacctgctg gagaccctcg cccagtcgtc gggggtcgcc ctgtcgagag aggccctggt
     1981 gaaaagcgtc ctgaaaaggt ccttctcccc ctttgacagg agcctcgatg tccatatcag
     2041 caacctccgc cggaagatcg gcacctacc
//
LOCUS       DAOSRG010000214         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_49563_length_2063_cov_6.760956, whole genome shotgun sequence.
ACCESSION   DAOSRG010000214 DAOSRG010000000
VERSION     DAOSRG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49563_length_2063_cov_6.760956"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1596)
                     /locus_tag="PLM30_06295"
     CDS             complement(178..1596)
                     /locus_tag="PLM30_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOR54816.1"
                     /translation="MSQGEQKARESWGTRFGFIMAAAGSAIGLGNIWRFPYLTGMNGG
                     GAFIVIYLGCVLFVGLSIMLGEFVVGRGTGLASVGAFKSRHRNWTFVGVLGVISSFFI
                     YCFYPVVGGWAVAYMVKSLTGLLSNPGAIADYFGAFITAPLEPVIWTLIFLAVNLFVV
                     AKGVSGGIELTSKILMPMLFGLLVIIIIKSLSLPGSGAGLTFLFKPDWSVVTGKTFLA
                     ALGQAFFSLSLGMGVMITYGSYLKKDEHLPSDALTVVSMDTLAAILAGIAIFPALFAF
                     GVEPTAGTGLVFVVVPQVFAQMGGIGVLFSFVFFLALAIAALTSSVGLLETVVAYFMD
                     EKGMSRKKATYISSAVTTVICILSSLSLGVMSGFSVFGVGVFDFFDILTDKIFLAIGG
                     MCLAIYVGWVLNKEEVKLEATNRGALPFALFETWYAVIKYVIPVLVAVVAVSGIMAIE
                     QQSLMIFGVVVIGVLALFSKKL"
     gene            complement(1923..2051)
                     /locus_tag="PLM30_06300"
     CDS             complement(1923..2051)
                     /locus_tag="PLM30_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HOR54817.1"
                     /translation="MERSGAEVILAELPVGEGVGLAVRDRLARAASGAEGPLDHWP"
BASE COUNT          472 a          612 c          580 g          399 t
ORIGIN      
        1 tcgcccgaga cgccgagccc gaaactgatc ctggtgggca ggatgaaatc aaaactgctc
       61 atgaaactca ttgtcatcac cacttgttcc ttgaattgta atgatcccag tacatttttc
      121 gatgaagaag gaggacccgg ctttgcggcc gggtcctcga acctcacggt gcagctgtta
      181 cagttttttg gagaacagcg ccaaaacgcc gatgactact accccgaaga tcatgaggct
      241 ctgttgctcg atcgccatga tccccgatac ggccaccact gccacgagga cggggatgac
      301 gtactttatc acggcatacc atgtttcgaa gagggcgaag gggagcgccc ccctgttcgt
      361 ggcctcgagc ttcacttctt ccttgttcag gacccagcca acgtaaatgg caaggcacat
      421 cccaccgatg gcgaggaata tcttgtccgt caggatgtcg aagaagtcga agacgccgac
      481 cccgaacacg ctaaagccgg acatcacgcc cagggagagc gaggagagga tgcatattac
      541 cgtcgtgaca gcggaagaaa tgtaggtcgc tttcttcctg gacatgccct tctcgtccat
      601 aaagtaggcg acgacggtct cgagcaggcc caccgaagag gtcagggcgg cgatggccag
      661 ggcgaggaag aacacgaagg agaagagcac ccctatgccg cccatctggg cgaacacctg
      721 ggggacgacc acgaagacta ggccggtccc ggctgtgggc tcgacgccga aggcgaagag
      781 ggccgggaag atggctatgc cggcaaggat ggccgccagg gtgtccatgg agaccaccgt
      841 cagggcatcg ctggggaggt gttcgtcctt cttaaggtaa ctgccgtagg ttatcatgac
      901 gcccatgcca aggctgagcg agaagaaggc ctgccccagg gccgccagga aggtcttgcc
      961 cgtcacgacc gaccagtcgg gtttgaaaag gaacgtgagg cccgcccccg aaccgggaag
     1021 gctgaggctt ttgattatga tgatcaccaa cagaccgaac agcatgggca tgagtatctt
     1081 gctggtcaac tcgataccgc cggagacgcc cttggcgacg acaaacaggt tcaccgccag
     1141 gaaaatgagc gtccagatca cgggctcgag gggcgccgtg atgaaggccc cgaagtagtc
     1201 agcgatcgcc cccgggttag atagcagccc ggtcagcgac ttgaccatgt aggccacggc
     1261 ccagccgccc accacggggt agaagcaata gatgaagaat gagcttatga ccccgaggac
     1321 ccctacgaag gtccagttcc tgtgcctgct tttgaaggct ccgacggagg caagcccggt
     1381 ccccctgccg acgacgaact ctccgagcat tatgctgaga ccaacgaaaa ggacgcaacc
     1441 gaggtagatc actatgaaag ccccgccgcc gttcatcccc gtcaggtaag ggaacctcca
     1501 gatgttcccc aacccgatcg ccgaacctgc cgccgccatg atgaagccga acctggtacc
     1561 ccaactctct cttgccttct gttccccttg tgacatttta cgactccctc cctttttatt
     1621 gatctagtcg tatttcagcg gctccgccgc tgtacaaccc ttgaaataaa aacgttgagc
     1681 gtcgagcttg cggcctgctt tgagccctcc gtgcccctcc ggatgaaagg aagagcttgc
     1741 ttcctttcgg cacggccgcc gaaaggagat catattatat catacatcat taacgttatg
     1801 ataaacaagg ggatatttca acttctgcgg agggatctat cgtcggtatt tctcgaatat
     1861 tcgcgttttt tatctgtttt tctcaaaaaa acggcctggt tgagataggg cccggatcgt
     1921 tctcatggcc agtggtccag ggggccttcc gcccccgatg cggccctggc cagcctgtca
     1981 cggaccgcca ggcccacacc ctcccccacg gggagttcgg ccagtatcac ctcggcgccg
     2041 gagcgttcca gttccctcag ggc
//
LOCUS       DAOSRG010000215         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_50334_length_2041_cov_9.976838, whole genome shotgun sequence.
ACCESSION   DAOSRG010000215 DAOSRG010000000
VERSION     DAOSRG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50334_length_2041_cov_9.976838"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLM30_06305"
     CDS             <1..618
                     /locus_tag="PLM30_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54818.1"
                     /translation="KTDLVYEEIKKLILRGELSSDTPISENHLARRFEISRTPIREAL
                     KRLHMEGFVRIIPNQGVFVRDVSINEVKDIYDLRIALEEFVIKELTGILSQDDLSLLE
                     GNLARQEEALLKNDAFAFHEEDRNFHDYFMKAYGNAMITDFITNLRDRIEGININMLK
                     QPGNMRLFWGEHKKILEALHKRDAEGATREMDEHLKGGKDRLLRG"
     gene            762..1775
                     /locus_tag="PLM30_06310"
     CDS             762..1775
                     /locus_tag="PLM30_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOR54819.1"
                     /translation="MRKLLTGILALVLVFGLASVDTAQAALKGEYKLTMNVTTDTAWG
                     QGGVKFAELVKDYTGGKVNVKVYPSAQLVGGDQMRQAEMVSSGAIDFMLNSTINIAPI
                     IPECNAFSLPFMFPSYESVDAVTGSAAGQMVLDALEKHNMVGLAWGENGFRELTNNVK
                     PVAHPDDMKGLKIRVVTPIYIDIMRALGANAIAMNWGEVFTSLQQGVIDGQENPIVGI
                     IIPNKIYEVQKYLTNWHYSYDALILAVNKNVWESFDPDIKEQIKKAAVDSMRWQVHDV
                     SRVGLGDGIEFLKSQGMTVTDPTPEQLAAFREMTKAVYEKWSANVGADIVKAFEEAVS
                     SFK"
BASE COUNT          495 a          548 c          604 g          394 t
ORIGIN      
        1 aagaccgacc tggtttatga agagatcaag aagttgatcc tccgggggga gctctcaagc
       61 gacacaccga tctcggagaa ccacctcgcc aggaggttcg aaataagccg gactcctatc
      121 cgcgaggcgc taaaacggct ccatatggag ggcttcgtca ggatcatccc caaccagggc
      181 gtattcgtcc gcgacgtctc cataaatgaa gtaaaggata tatatgacct gcggatagcc
      241 ctggaagagt tcgtaataaa ggaattgacc ggcatccttt cacaagatga cctttcactg
      301 ctcgagggca acctcgccag gcaggaggaa gccctcctga agaacgacgc cttcgccttc
      361 cacgaggagg accgcaattt ccacgattat ttcatgaaag cctacggcaa cgccatgata
      421 acggacttca taactaatct ccgcgaccgg atcgagggga tcaatataaa tatgctcaag
      481 cagcccggca acatgagact tttctggggt gagcacaaaa agatcctgga agcccttcat
      541 aagagggacg ccgagggggc gaccagggag atggacgaac atcttaaagg aggcaaggac
      601 aggcttctca ggggctgatc caccgcgtga agcgcggcgg tgacccatac cgggcaggag
      661 gagggatggc gaggatagcg aaagggtggt ttccccggcc gggggatcaa aaaacctgtg
      721 agggaggtga actgtccggg ttcaccggac aggacgtgaa gatgaggaaa ttgctgacag
      781 gaatactggc gctggttttg gttttcgggt tggcaagcgt tgacacggcc caagcggcac
      841 tcaagggcga gtacaagctc accatgaacg tcaccaccga tacagcctgg ggccaggggg
      901 gggttaagtt cgccgagctg gtcaaggatt acaccggcgg caaggtgaac gtcaaggtct
      961 atcccagcgc ccagctcgtc ggcggcgacc agatgcgcca ggccgagatg gtctccagcg
     1021 gtgcgataga cttcatgctc aactccacga taaacatcgc cccgatcatt cccgaatgca
     1081 acgccttttc gctccccttc atgttcccca gttacgaatc cgttgacgcc gtgaccggat
     1141 cggcggcggg ccagatggtc ctggacgccc tggagaagca caacatggtc ggactggcgt
     1201 ggggcgagaa cgggttccgt gagctcacca acaatgtcaa acccgtagcg caccctgatg
     1261 acatgaaagg cctcaagatc agggtcgtga cgcctattta tatcgatatc atgagagccc
     1321 tcggcgcgaa tgccatagcc atgaactggg gcgaggtctt cacctcgctg cagcaaggcg
     1381 ttatcgacgg gcaggagaac cccatcgtcg ggataataat cccgaacaag atctacgagg
     1441 tgcagaagta cctcaccaac tggcactatt cctacgacgc cctgatcctg gccgtcaaca
     1501 agaatgtgtg ggagagcttc gaccccgata tcaaggagca gatcaagaag gccgccgtcg
     1561 attcaatgcg atggcaggtc cacgacgtga gccgcgtagg cctgggcgac ggcatcgagt
     1621 tcctgaaatc ccagggtatg acggtgaccg atcctacccc ggagcagctt gccgcgttcc
     1681 gcgagatgac caaggccgtc tatgaaaagt ggtccgccaa cgtcggcgcc gatatcgtaa
     1741 aagccttcga ggaggccgtt tcgagtttca aataatgggt catggtacgg gggccgctgc
     1801 cgcgacccct ctgtcccctt cccgggggag ggatctgtcg gggatcaacc gataggaagg
     1861 gaacggtgct ggggatgttc aagctgcttg acaggataga agagtggacc tgcgttgcga
     1921 tgcttttttt catggccatt ctggcctttg ccaacgtgat agtaaggtac ctgacgacct
     1981 tttctttttc cttctcggaa gaactgctgg ttaacctctt tgtctggata acgatgctcg
     2041 g
//
LOCUS       DAOSRG010000216         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_50661_length_2032_cov_9.582701, whole genome shotgun sequence.
ACCESSION   DAOSRG010000216 DAOSRG010000000
VERSION     DAOSRG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50661_length_2032_cov_9.582701"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLM30_06315"
     CDS             <1..452
                     /locus_tag="PLM30_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54820.1"
                     /translation="PLFAYTHAGHAVSCASAEAMIKVVRDEGLAAAAVEKGQVIADWF
                     RSRAERYPFIGDIRQKGLLMGVEVVSDREKRTPDKAMAMKISWAAWERGLIMITFGKD
                     GNVLRLAPPLNIPDELLQEALGIMDASLEDAASGKVPAAILPLLKGW"
     gene            complement(513..1334)
                     /locus_tag="PLM30_06320"
     CDS             complement(513..1334)
                     /locus_tag="PLM30_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR54821.1"
                     /translation="MNLPKVVLWIVIIYGVFCGGIFLLQDKMIYQPWGEITATPSRIG
                     LGFENISFEASDGVRLHGWFIPAADPSGRALLFFHGNAGNISHRLESIMIFHDLGFDV
                     LIFDYRGYGKSEGRPSEEGLYRDGRAAFDFLVERGYTRDRVVVFGRSLGGAVAARVSL
                     EKFPGALVLESSFTSLADMAKKVARFFPVRRLLKSEYPTESILGGIRCPVLVVHSPDD
                     EIIPFSQGMKLYEAVTGPKAFFEIKGDHNGGFILTGEAYRKGLGEFLSGIGGVER"
     gene            complement(1331..1951)
                     /locus_tag="PLM30_06325"
     CDS             complement(1331..1951)
                     /locus_tag="PLM30_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOR54822.1"
                     /translation="MTGRVRIRDSLSFGWRTFRRNTLFFIVLLVILGIPLAILGALLG
                     GLPPGGADPSRLLLEVALWALRSLAAMVIIVVSLSFLDRGAFDMERLKRMKPLFIPFI
                     VGTFLFFSLVSVGVVLLVLPGVYIAIRFQFTPYLIIDGGMGPIDAFKAAWAMTRGLGL
                     ELFLLLLSIVGLNILGAVPLGLGLLITVPVTFLAHAMVYRDLASLP"
BASE COUNT          451 a          634 c          623 g          324 t
ORIGIN      
        1 cgccgctgtt cgcctacacc cacgcaggcc atgccgtcag ttgcgcttcg gcggaggcca
       61 tgatcaaggt cgtgcgtgac gaaggactcg ccgccgcggc ggtggaaaag ggacaggtca
      121 tcgccgactg gttcaggtcg agggccgagc ggtacccctt catcggtgac attcggcaga
      181 aggggctgct gatgggtgtc gaggtggtat cggacaggga aaagagaacg cccgacaagg
      241 ccatggcaat gaagatatcc tgggccgcct gggagagggg cctcataatg atcaccttcg
      301 ggaaggacgg caacgtgctg cggctggccc cgccgctcaa catacccgac gaactcctcc
      361 aggaagccct ggggataatg gatgcctccc tcgaggacgc ggcgtcaggg aaagtgcccg
      421 cagccatcct gccgctcctc aagggctggt agcaacaacc caactccttc ttctccatgg
      481 gaagcggccc ggcgacctgt ccgggccgtt ttctatctct ccaccccgcc gatgccggag
      541 aggaactctc ccagcccctt ccggtaggcc tcgccggtca ggatgaagcc cccgttgtgg
      601 tcgcccttta tctcgaagaa ggccttgggc ccggtgaccg cttcgtaaag cttcatcccc
      661 tgggaaaagg ggatgatctc gtcgtccggg ctgtggacca ccagaaccgg gcatcggatg
      721 ccgccaagga tgctctccgt ggggtactcg cttttcagga ggcggcggac cgggaagaag
      781 cgggcgactt tcttcgccat atcagcaaga gaagtaaaac tcgactccag caccagggcc
      841 cccgggaact tctccagcga gacccgcgcc gcgacggccc ccccgaggga ccttccgaag
      901 actaccacgc gatcacgggt gtagcccctt tccaccagga agtcgaaggc cgccctcccg
      961 tcccgataga ggccttcctc ggaggggcgc ccctcgctct tgccgtaacc ccggtagtcg
     1021 aagatgagga catcgaaccc caggtcgtgg aatatcatta tcgactcgag cctgtgggag
     1081 atgttgcccg cgttgccgtg gaaaaaaaga agggctcgtc cggaggggtc cgccgctggg
     1141 atgaaccagc cgtgaaggcg caccccgtcg gaggcctcga aggagatgtt ttcaaaaccc
     1201 aggcctatcc gtgagggggt ggcggtgatc tcaccccagg gctggtagat catcttgtcc
     1261 tgcagcagga atatcccccc gcaaaagacc ccgtaaataa taacgatcca taagactacc
     1321 ttgggcaggt tcatggcaac gaggccaggt cccggtaaac catggcgtgg gccaggaagg
     1381 tcactggcac ggtgatcagc agcccgaggc cgaggggtac ggccccaagg atattcaaac
     1441 cgacaatgga aagcagcaaa aggaaaagtt ccagcccaag gccccgggtc atcgcccagg
     1501 cggctttgaa ggcgtcgatg ggccccatcc ccccgtcaat gatcaggtag ggcgtgaact
     1561 ggaaccggat ggctatgtag acaccgggta acacaagaag gactaccccc acgctgacga
     1621 gggagaaaaa aaggaaggtc ccgactatga aagggatgaa caggggcttc atccgcttca
     1681 accgctccat gtcaaaggca cccctgtcca ggaaggagag gctgaccacg atgatgacca
     1741 tggcggccag ggacctgagg gcccagaggg cgacctccag caaaagcctg gaaggatccg
     1801 ccccgcccgg cggtaaccca cccagaaggg ccccgaggat cgccaggggg atgcccagta
     1861 tcaccaacag gacaatgaag aaaagggtat tccgcctgaa ggtcctccag ccgaaggaca
     1921 aggagtccct gatccgaacc ctgccggtca tgacaacctc ccccgggcga atggaccgcc
     1981 ccctggtaat ttttttcagg ccctcgcgta cacccgggcc tcctcttgtc cg
//
LOCUS       DAOSRG010000217         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_50984_length_2023_cov_5.991362, whole genome shotgun sequence.
ACCESSION   DAOSRG010000217 DAOSRG010000000
VERSION     DAOSRG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50984_length_2023_cov_5.991362"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..698
                     /locus_tag="PLM30_06330"
     CDS             192..698
                     /locus_tag="PLM30_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR54823.1"
                     /translation="MGLAREKTKKNLEEAFSGESQANRKYLAFAKKADEEGIHQVGRL
                     FRAVAEAETIHANSHLRALGMIGSTAENLDAAIAGETYEFTSMYPPFIEDAAMEGDKQ
                     AERSFRLANEAEKVHAGLFRKARESLLSAGDVDYYVCSVCGYIAEEEAPDTCPLCGAK
                     KEAFKQVE"
     gene            728..1240
                     /gene="tpx"
                     /locus_tag="PLM30_06335"
     CDS             728..1240
                     /gene="tpx"
                     /locus_tag="PLM30_06335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOR54824.1"
                     /translation="MERKGIVTMKGAPVTLLGPELAVGEKVPGFTVLDRSLKEVTLDD
                     FEGKIKVISVTPSLDTPVCDLQAHWFNEEAASLPGDVAVLNISMDLPFAIDRFCIAGG
                     IDSIEVLSDHRDASFGQRWGVLIKELRLLARSVFIIDTDNILRYKEIVAEATTPPDYD
                     SALAALKALR"
     gene            1340..>2023
                     /locus_tag="PLM30_06340"
     CDS             1340..>2023
                     /locus_tag="PLM30_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54825.1"
                     /translation="MYKVRVLNSEDLKKLLDIKDVITAVETAYEQYSRGEAELFPVIA
                     HEFERGSRELDIKSGFLTGTGLAGLKVIGYVADNPEKRRIPAISGIVIVLSLETGRPV
                     GMLDGMVITNIRTGAAGAIGAKHLAREGSSNILVVGTGAQGRVQVQGLTKVLPGIEVI
                     RVAGRDPENLSRFVGEMSALYPQIHFQESSMGGLQEAVSLSDVVVSCTPSHEPLIRAE
                     WVRPGTHINA"
BASE COUNT          457 a          521 c          625 g          420 t
ORIGIN      
        1 cggtactgag gatccatacg gacctcatgg gtcccatcct cgtgacccag gagaaccccg
       61 acctttacga cacggcctat ggggtcctgg cggacctgct aagcctgaag caaggataat
      121 aacgatttcc tgaagggact tgcgtcgggc ggacttaagg ggtatatttg aaacaatttc
      181 aaaaaggagg gatgggtttg gccagggaaa agacgaagaa gaatctggag gaagcttttt
      241 ccggggaatc acaggccaac cgtaagtact tggcttttgc gaaaaaggcc gacgaggaag
      301 gtatccatca ggtaggtagg cttttcaggg ctgtcgccga ggctgagacg atccatgcta
      361 actcgcattt gagggcttta ggcatgatcg gctctaccgc tgagaacctc gacgctgcca
      421 ttgccggcga aacctacgag ttcacgagca tgtacccgcc gtttattgaa gatgcggcga
      481 tggaagggga taagcaggcc gagagatctt tccgcctcgc caatgaagct gaaaaagtcc
      541 atgcaggcct gttccggaag gccagggaga gcctgctttc ggcaggggac gtcgattatt
      601 acgtgtgttc cgtctgcggt tatatcgccg aagaggaagc gcccgacacc tgtcctttat
      661 gcggggccaa gaaagaggct ttcaaacagg tcgagtaagg tcgacctttt caaaggaggt
      721 tgccgtcttg gaaaggaagg gtattgtaac gatgaaagga gctccggtga cgctgctggg
      781 cccggagctg gccgtcgggg aaaaggtgcc cggttttaca gtccttgacc ggtcactcaa
      841 ggaagtgacc cttgatgatt ttgagggaaa gataaaagtc atttcggtca caccttctct
      901 cgacactccg gtctgtgatc tccaagccca ctggttcaac gaggaagctg catcgcttcc
      961 cggcgacgtc gccgtactca acatcagcat ggaccttccc ttcgccattg acaggttctg
     1021 cattgccggc ggcatcgatt ccatcgaggt cctttcagac caccgggatg cgtccttcgg
     1081 acagcggtgg ggtgttctca tcaaggaact ccgcctgctc gcaagatcgg tgttcataat
     1141 cgacacggac aacattctcc gctacaagga gatagtggcc gaggccacga cgccccccga
     1201 ctatgattca gccctggccg cgttgaaagc gcttcgataa agccgatctt tgaggttgaa
     1261 gggccccctg gggccctttt ttgttggtgg agatggggta tattatcagt ggtagccggt
     1321 cccggaaggg gttgaaggga tgtacaaggt cagggtcctg aacagcgaag atctgaaaaa
     1381 actcctggac atcaaggacg tcatcaccgc cgtggagacc gcttatgagc agtattccag
     1441 gggcgaggcc gaactatttc ccgttattgc ccatgaattc gagcgagggt cccgggagct
     1501 ggatatcaag tcggggttcc ttacaggaac aggcctggcg gggctcaagg tgatcggata
     1561 cgtggccgac aacccggaaa agcggaggat cccggccatt tcagggatcg tcatcgtgct
     1621 cagccttgaa acggggagac cggtgggcat gctggacgga atggtcatca ccaatatcag
     1681 gactggagcc gccggtgcca tcggcgcgaa gcatcttgcc agggaggggt ccagcaatat
     1741 cctggtggtt ggcaccggcg cccagggaag ggtccaggtg caaggtctga ctaaggtcct
     1801 cccggggatc gaggttatac gggtagcggg gagggatccc gagaatcttt cccggttcgt
     1861 gggcgaaatg tcagccctct acccccagat acactttcag gagagctcga tgggagggct
     1921 ccaggaggcg gtctctctgt ccgatgtcgt cgtgagctgc accccttccc acgaaccctt
     1981 gatcagggcg gaatgggtcc gacccgggac ccacattaac gcg
//
LOCUS       DAOSRG010000218         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51104_length_2020_cov_5.042748, whole genome shotgun sequence.
ACCESSION   DAOSRG010000218 DAOSRG010000000
VERSION     DAOSRG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51104_length_2020_cov_5.042748"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLM30_06345"
     CDS             complement(<1..479)
                     /locus_tag="PLM30_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOR54826.1"
                     /translation="MEFQKVRLVRSAVTNPWDNLAREEQLVNLCEEGEAILYLWQNAH
                     TVVVGRNQNPWAECCLDLLESEGGKLARRSTGGGAVYHDLGNLNYSFILPRKAYNMER
                     QLAVILGALRRLGIIAEFSGRNDLVIAGRKFSGNAYQLTRDKGLHHGTLLFSADMAKL
                     "
     gene            complement(615..1745)
                     /locus_tag="PLM30_06350"
     CDS             complement(615..1745)
                     /locus_tag="PLM30_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54827.1"
                     /translation="MVVKIEPFGVEQWMGRWETLAVYNIAETCVDSLVLYELLEMSGR
                     KEDIIVDLLGTRMTYGDIMGSERLRGLIADLYEGGDPERVLAMNGGSAANFLALFTLV
                     EPGDRVISVWPTYQQLTSIPEAFGAEVEILPLRPENGWLPDPDELRKMAGGGIDLICL
                     NNPNNPTGSLMDGDLLEEIALIARECGAWVLCDEAYRGLNHEPGVPAPSMAEIYEKGI
                     STGSFSKVFSLAGLRSGWMTGPAEFIEEAFCRRDYTTISCGPLVDALSVAALENRGKI
                     MERNLGIIRESLAILEAWIDSNARFHWVKPRAGTTAFPGYNYDIPSDEFCLELLAATG
                     TFLLPGSCFGIDKRLRVGYAYGTETLKRGLDSISEWLESRFS"
BASE COUNT          367 a          608 c          629 g          416 t
ORIGIN      
        1 agcttggcca tgtcggcgga gaaaagaaga gtcccgtggt ggagcccctt gtccctggtc
       61 aactggtagg cgttgccgga gaacttcctg ccggctatga ccaggtcgtt cctgccggag
      121 aattcggcga tgatgccgag cctgcgaagg gcgccgagga tgacggcgag ctgacgttcc
      181 atgttgtagg ccttcctggg aagtatgaag gagtagttga ggttaccgag gtcgtgatag
      241 actgcgcccc cgccggtgga acggcgggcg agttttccgc cctccgactc gaggaggtcc
      301 aggcagcatt cggcccaggg gttctggttt cgccccacca ccacggtgtg ggcgttctgc
      361 cagaggtaga ggatcgcctc gccctcttcg cagaggttca ccagctgctc ttccctggcc
      421 aggttgtccc aggggttcgt tacagcggaa cgcaccagcc gtaccttttg aaactccatg
      481 gccactcccc gggcggtcga tgatcgccgc tgacctaggc tccaagttta ccatgtaata
      541 cggattgggg gtataggtgc ttgtatgatg aaattacagg atgattcgaa tatatccgtc
      601 ggagcgtgcg cctctcagga aaaacggctt tcaagccact ccgatatcga gtccagtccc
      661 cgcttgagcg tttccgtccc gtaggcgtag cccacccgga gcctcttgtc gatgccgaag
      721 cagctcccgg ggaggaggaa ggtccccgtg gccgcaagca gttcaaggca gaactcgtcg
      781 gagggaatgt catagttgta accgggaaag gccgtcgtcc ccgcccgggg tttgacccag
      841 tggaaacggg cgttcgagtc gatccacgcc tccagtatgg cgaggctctc ccggatgatg
      901 cccaggttcc tctccatgat cttcccgcgg ttttccaggg ctgccaccga cagggcgtcc
      961 acgagggggc cgcaacttat ggtggtgtag tccctcctgc agaaggcctc ctcgatgaat
     1021 tccgcgggac ccgtcatcca cccgcttctc aggccggcca gggaaaagac ctttgaaaaa
     1081 ctccccgtgc ttatgccctt ttcgtagatt tccgccatgg agggcgcggg cacgccgggc
     1141 tcgtggttca gaccccggta ggcctcgtcg cagaggaccc aggcaccgca ttcacgggcg
     1201 atgagggcga tctcttccag caggtctccg tccatgagag agcccgtggg gttgttgggg
     1261 ttgttgaggc agatcaggtc gatccctccc ccggccatct ttctcaattc gtccgggtcg
     1321 ggaagccagc cgttttcggg cctcagggga aggatttcca cctccgcgcc gaaggcctcg
     1381 gggatgctcg tcaattgctg gtaggtgggc caaacgctta tgaccctgtc gccgggctcc
     1441 accagggtga agagggccag gaagttggcc gccgatcccc cattcatcgc gaggaccctt
     1501 tccggatcgc cgccctcgta gagatcggcg atcaggcccc ttagcctttc actccccatg
     1561 atgtcgccgt aggtcatcct ggtgccgagc aggtctacga tgatatcttc cttcctgccc
     1621 gacatctcga gcagttcgta aagcaccagg gaatccacgc aggtctcggc tatgttgtag
     1681 acggccagcg tctcccagcg tcccatccac tgttccacgc cgaagggttc gatcttcacc
     1741 accatcactc cccgctttga aaaaacggcc tcgccaagag tatgcacgtt ccctggccgg
     1801 aagtgaaggg cggtggggtt ttgaaggcgc gccccaggct ggtacaatcc tttttgtacc
     1861 atgacccgat ccccggcctg taagaaaggc gtgagcggcc gcggggatcc ccgagggagg
     1921 gtgctcatca tggagtggaa ggaccgtttc agcgcgattg ttcgagacag gctcgaggaa
     1981 tcggccatct cggtggtgat gagggaggcg gtccgccagg
//
LOCUS       DAOSRG010000219         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51197_length_2018_cov_8.924605, whole genome shotgun sequence.
ACCESSION   DAOSRG010000219 DAOSRG010000000
VERSION     DAOSRG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51197_length_2018_cov_8.924605"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLM30_06355"
     CDS             complement(<1..202)
                     /locus_tag="PLM30_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV aminotransferase"
                     /protein_id="HOR54828.1"
                     /translation="MAICYTDGVFVQSGEASLPLSDFAFQRGVAVFETIRTYGGRPMA
                     LTPHLERLAASAAGSRIAMPIPI"
     gene            340..1428
                     /gene="buk"
                     /locus_tag="PLM30_06360"
     CDS             340..1428
                     /gene="buk"
                     /locus_tag="PLM30_06360"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOR54829.1"
                     /translation="MNPEILAVNPGSTSTKIAWFSGEERRWQVTVDHDPAVISSFPKL
                     ADQYQFRMETIEEAARANGSDLNALDAVVGRGGLLEPIPGGTYRVDEDVLRDLWRGKP
                     WEHASNLGGIIAEAIAEPRGIPSFIVDPVAVDELDDLSRLTGLPELPKRSLGHALNIK
                     AAVRMAARDLERPWDELDCLVAHLGGGITVCAHRHGRMVDLNNANEFGPFSPERAGGL
                     PAGDLARLCYSGDYTMASLRRRLVGEGGLKAYLGTSDMREVDARVEAGEERAGLVFRA
                     MALQVAKEIASFAATLSGEVDAVILTGGIARDVGFVELVTSRVQWIAPVLVYPGEDEM
                     RALAQGALRVLSGEEEALVYSENLTGRA"
     gene            1433..>2018
                     /locus_tag="PLM30_06365"
     CDS             1433..>2018
                     /locus_tag="PLM30_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOR54830.1"
                     /translation="MPFETLDFLVEQSRSGKAVVLAVAAPHGEDILEAVAEARERGVA
                     SPILFGDRARIEKVASEFSIDTSSWAVVQEADEGRAAELAVKAVSSGEADLLMKGNVK
                     TATLLKAVLNKEWGLRSGSLLSHVFLFQIPRMGRVFCLTDGGMSMYPDLEAKKAIIEN
                     AVECYHKLGVECPRVAALAAVETVNQDMPCTVDAA"
BASE COUNT          373 a          616 c          706 g          323 t
ORIGIN      
        1 cgatgggtat gggcatggcg atcctcgacc cggcagcgga ggcggcgagc ctttccaggt
       61 ggggggtgag ggccatgggc ctcccgccgt aggtcctgat ggtctcgaac acggcgacac
      121 ccctctggaa ggcgaagtcg gaaaggggca gcgaggcctc gccggattgt acgaagacac
      181 cgtcggtgta gcagatggcc atgaaggtgg accccccggg gttttgtctt ttgggacggg
      241 gaacagtata ctccatccgg ccgggtttca gggattgaag aagtgtataa ttcccatgaa
      301 cggcctagaa ccccttcaaa ggaggcggag ctcgaagcga tgaacccgga aatactcgct
      361 gtcaaccccg gctcgacgag caccaagata gcctggttct cgggcgaaga gcggcgatgg
      421 caggttacgg tggatcacga cccggcggtg atatcctcct tccctaagct ggcggaccag
      481 taccaattca gaatggagac catcgaggag gccgccaggg ccaatgggag cgacctcaac
      541 gccctcgacg ccgtggtcgg gcgggggggg cttctcgaac ccatacccgg cggaacctac
      601 agagttgatg aggacgtcct gcgcgacctc tggcggggca agccctggga gcacgcctca
      661 aaccttggcg ggatcatcgc cgaggccatc gccgaaccca gggggatccc ctccttcatc
      721 gtcgaccccg tcgccgtgga cgaactggac gacctttccc ggctgacggg tttgccggaa
      781 ttgcctaaga ggtccctcgg gcacgccctc aatatcaagg cggctgtaag gatggccgcc
      841 agggacctcg agaggccctg ggacgagctc gattgcctgg tcgcccacct gggcgggggc
      901 ataacggtct gcgcccaccg ccatgggagg atggtggacc tcaacaacgc caacgagttc
      961 ggccccttct cccccgagag ggcgggaggc ctccccgcgg gggacctggc ccgcctctgc
     1021 tacagcggcg actacaccat ggcaagcctt cgaaggcggc tcgtgggcga gggcggcctg
     1081 aaggcctacc tcggcacgag cgacatgagg gaggtcgacg ccagggtcga ggcgggggaa
     1141 gagagggccg gcctggtctt cagggccatg gcgctccagg tggccaagga gatagcctcc
     1201 ttcgcggcga ccctctccgg cgaggtcgac gccgtgatcc tcaccggggg catcgcccgc
     1261 gacgtgggct ttgtcgaact ggtcacatcg agggtccagt ggatagcccc ggtgctggtc
     1321 tacccgggcg aggacgagat gagggctctc gcccaggggg ccctgagggt cctctcgggc
     1381 gaggaagaag ccctggtcta tagcgagaac ctgacgggga gggcatgaag ggatgccgtt
     1441 tgaaacgctc gatttcctgg ttgaacaaag ccggtccggc aaggccgtgg tcctggcggt
     1501 ggctgcgccc cacggggagg atatcctcga ggccgtcgcc gaggcccggg agaggggcgt
     1561 cgcctcgccc atactcttcg gggacagggc gaggatagaa aaggtcgcct cggagttctc
     1621 cattgatacc tcctcctggg cggtcgtgca ggaggccgac gagggcaggg ccgcggaact
     1681 ggcagtgaag gccgtttcct cgggcgaggc ggacctgctg atgaagggca acgtcaagac
     1741 cgccaccctg ctgaaggccg tcttgaacaa ggaatggggg ctccgttccg gttccctcct
     1801 ctcccacgtc ttcctcttcc agatccccag gatggggagg gtcttctgcc ttaccgacgg
     1861 gggcatgtcg atgtaccccg acctggaggc taagaaggcg ataatcgaga acgccgtgga
     1921 atgctaccac aagctcggcg tcgagtgccc cagggtcgcg gccctggccg ccgtcgagac
     1981 ggtcaaccag gacatgccct gcaccgtcga cgcggcgg
//
LOCUS       DAOSRG010000220         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51201_length_2018_cov_7.855833, whole genome shotgun sequence.
ACCESSION   DAOSRG010000220 DAOSRG010000000
VERSION     DAOSRG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51201_length_2018_cov_7.855833"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLM30_06370"
     CDS             complement(<1..1205)
                     /locus_tag="PLM30_06370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR54831.1"
                     /translation="MYDLVILGGGPGGLRAAELAAAAGMNTVLVEKDRLGGVCVNSGC
                     IPTKALYASIIGGKGPREGLWSRVESVVDKLRQGSATSLRMAGVKYLRGTGKVTRWEG
                     GKRISVTKADGSVEEIAGARLLLATGARSVRPPFRGGDLPGILTGDRAITEPSLWDPE
                     RNSPVKTVAILGAGVIAVEMASLLQGMGKEVVLLKRSDQVLRRMDGDIKKKTVQILKK
                     RGTVIKDHVRLREASLKEGRLSLEGTTGEEPFVVACDRLIIASSMEPVLDGFGLEESP
                     VRVERGAIAVDPSQGTTVEGVYAVGDCTGGAMLAHLAEYQALAAVSSMTGGDYQVDYD
                     AVPACVFMNPEVGAVGLTEEEARARSEEVVTAKAYFGANGMALALGESDGFVKVVARA
                     SDRTLLGVH"
     gene            complement(1286..>2018)
                     /locus_tag="PLM30_06375"
     CDS             complement(1286..>2018)
                     /locus_tag="PLM30_06375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807709.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOR54832.1"
                     /translation="NLNAIMGKARPGDMGFDVLHLNLHKTFGTPHGGGGPGAGPVGVK
                     AELEPFLPSPVVVHKGGRYTFTGKDERPRSIGRVRSFYGNFGVLVKAYAYIRALGPEG
                     LKEASEMSVLNANYLAKKLGGLYPLAHKGHCMHEFVLNGGSLKHETGVSTLDVAKAVI
                     EAGFHPPTIYFPLIVPEAIMVEPTETESVETLDRFAEAMEDIVAKARSEGPEAFHGLP
                     TTTPISRPDETTAARNPILRWQFEE"
BASE COUNT          284 a          681 c          681 g          372 t
ORIGIN      
        1 atgtggacgc ccagcagggt cctgtccgag gcccgggcga cgaccttaac gaagccgtcg
       61 ctttcgccca gggccagggc catcccgttg gcgccgaagt aggccttcgc ggtgacgacc
      121 tcctcgctcc tggcgcgggc ctcttcctcg gtcagcccca cggcgcccac ctccgggttc
      181 atgaagacgc aggcggggac ggcgtcgtag tccacctggt agtccccgcc cgtcatcgag
      241 gagacggcgg cgagggcttg gtactcggcc aggtgggcga gcatggctcc ccctgtgcag
      301 tcccccacgg cgtagacccc ctccacggtc gtcccctggg atgggtccac cgctatggcg
      361 cccctctcga ctctaacagg gctttcctcg aggccgaagc cgtcgaggac cggttccatg
      421 ctcgaggcga tgatcagccg atcgcacgcc acaacgaagg gctcttcccc ggtggtaccc
      481 tccaatgaca gccgcccctc tttcagcgag gcttcccgaa ggcggacgtg gtcttttatc
      541 acggtccccc gttttttcaa gatctggacg gtcttcttct ttatatcgcc gtccatccgt
      601 cggaggacct ggtcggagcg cttgagcagg acgacttcct tccccatgcc ctggagtagg
      661 gaggccatct cgacggcgat gacccccgcg ccgaggatgg ccacggtctt cacgggcgag
      721 ttgcgctcgg ggtcccagag ggagggttcc gtgatggccc tgtcgccggt gaggatcccc
      781 gggaggtcgc cgccccggaa gggggggcgc acggagcggg cgcccgtggc cagcagcagc
      841 ctggcgccgg cgatctcttc cacgctgccg tcggccttgg tgacggagat cctctttcct
      901 ccctcccagc gggtcacctt gccggtcccc cggaggtact tcacgcccgc catgcggagc
      961 gatgtggccg agccctggcg gagtttgtcc actacggact ccaccctgga ccagagcccc
     1021 tccctgggcc ctttgccgcc gatgatggag gcgtagaggg ccttggtggg gatgcagccg
     1081 ctgttgacgc agacgccgcc gaggcggtcc ttttccacga ggacggtatt catgccggct
     1141 gccgcggcca gttcggcggc ccggaggccg ccaggcccgc cgccgaggat caccaggtcg
     1201 tacatgtcct tctctccttt tttcgggcct gctgaaggcc aaaggggcgc cctttttaaa
     1261 aggcgcccca gcctcgcggc cccggttatt cctcgaactg ccagcgaagg atcgggttcc
     1321 gggccgccgt ggtctcgtcg ggcctggaga tgggcgtcgt cgtcggcagg ccgtggaagg
     1381 cctcgggccc ctcggagcgg gccttggcca cgatgtcctc catggcctcg gcgaaccggt
     1441 cgagggtctc caccgattcc gtctccgtgg gttccaccat gatggcctcg ggtacgatga
     1501 gggggaagta gatcgtgggc gggtggaacc cggcctcgat gacggccttg gccacgtcga
     1561 gggtgcttac gcccgtctcg tgcttcagcg agccgccgtt cagtacgaac tcgtgcatgc
     1621 agtggccctt gtgggccagg ggatagaggc cgcccagctt cttggccagg tagttggcgt
     1681 tgagcaccga catctcggag gcttccttca gcccctccgg cccgagggcg cggatgtagg
     1741 cgtaggcctt cacgaggacg ccgaagttgc cgtaaaaact cctgaccctg ccgatggagc
     1801 ggggccgttc gtcttttccg gtgaaggtgt agcggccgcc cttatgaacg acgaccggcg
     1861 aggggaggaa gggctccagt tcggccttga cccctaccgg ccccgccccc ggcccgccgc
     1921 ctccgtgggg ggtgccgaag gtcttgtgga ggttcaggtg gaggacgtcg aaacccatat
     1981 cgccggggcg ggccttgccc atgatggcgt tcaggttc
//
LOCUS       DAOSRG010000221         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51291_length_2016_cov_7.645589, whole genome shotgun sequence.
ACCESSION   DAOSRG010000221 DAOSRG010000000
VERSION     DAOSRG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51291_length_2016_cov_7.645589"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLM30_06380"
     CDS             <1..672
                     /locus_tag="PLM30_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR54833.1"
                     /translation="FHDREPISRNEFLQMAGRAGRKGLFETGYVTWLKGSPQESRGSD
                     TGKIFRRLVSAQPESASVTLRPDYGKLLKGQRLIPDEAEYIASFSLPPRDVPTVEAEL
                     RAGLKFIDRSIREMVDLDWRERFREVLADIWYGEMEISENLEMAGLFLETGKADALTA
                     AQVINLFEKSYLKSLLKVKRFSNHLPSQYEVTGMHYLDRTVDEIDGTIYGFEEKIQEI
                     EESSS"
     gene            complement(753..2015)
                     /locus_tag="PLM30_06385"
     CDS             complement(753..2015)
                     /locus_tag="PLM30_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54834.1"
                     /translation="MLSEMFRISQETLEEINRFMLDPGNPDVQALLRVVAKYGTPEEI
                     NAKAREARCLSSLMGRLERMGSPYLEDLNWLESERDKGAFIPISEYRKKVLGGDPAGR
                     TFSESTSVTLEISALQYFPFLVAEAEQAIERKEIMPGRYIRVRKMKEQERDQGDTLAV
                     AAAMQIVGASYAETLDTKGTDGSNVHLGGPETITGYFGGIGQPNEHPLAWADELLYYY
                     TNYGINEVLNINPGTVLLGYLLHRLGIDIGFKISVFMGNDNPYAVLWTLMMARLFARE
                     DGSTPLVGFNLSNSVDVSTLMACADIRRRLGLEDKVRIEHHITETYKSIVRQPYNRRA
                     DLLEVARVYPNISAKHEGGEPEVEQTLAHPSDILDYFRDKAEVLASGDMDNLKTNFLK
                     KHEATNKTAEELTKAGLSFIAAQRLHAR"
BASE COUNT          355 a          618 c          642 g          401 t
ORIGIN      
        1 ttccacgaca gggaacccat ctccaggaac gaatttttgc agatggcggg gagggccggc
       61 aggaaggggc ttttcgagac gggctacgtg acatggctca agggctcgcc ccaggaatcc
      121 cggggctccg acacggggaa gatcttccgc cggctcgtgt cggcccaacc cgagagcgcc
      181 tcggtgacct tgaggcccga ctacgggaag ctgctcaagg ggcaaaggct catccccgac
      241 gaggcggagt acatagcctc cttctccctc cccccaaggg acgtgccgac cgtggaggcc
      301 gaactgaggg ctgggctgaa gttcatcgac cggtccatcc gggagatggt ggacctcgac
      361 tggagggagc gcttccggga ggtcctggcc gatatatggt acggcgagat ggagatcagc
      421 gagaacctcg agatggccgg cctcttcctg gagaccggca aggccgacgc cctgacggct
      481 gcccaggtga taaacctctt cgagaagagc tacctaaagt cactcctcaa ggtcaagcgc
      541 ttctcaaacc acctcccgtc ccagtacgag gtcacgggga tgcattacct ggaccgcacg
      601 gtggacgaga tcgacgggac gatctacggc ttcgaggaga agatccagga gatcgaggaa
      661 agttcgtcct gaccttctgg ccacttagcc cgaaaaataa agagaccggg tgaagtacca
      721 cccggtctcc gcttttttca aggcaccaat ggtcagcggg cgtggagccg ctgggcggcg
      781 atgaaggaga ggccggcctt cgtgagttcc tcggcggttt tgttcgtggc ctcgtgcttc
      841 ttgaggaagt tggttttgag gttgtccatg tcgcccgagg cgagaacctc cgccttgtca
      901 cggaagtagt ccaggatgtc cgaggggtgg gccagggtct gttcgacctc gggctcgcct
      961 ccctcgtgct tcgccgagat gttcgggtag acccgggcca cctccagcag gtcggcccgc
     1021 ctgttgtagg gctggcggac gatgctcttg taggtctcgg tgatgtggtg ctctatcctg
     1081 accttgtcct ccaggcctag cctcctgcgg atatcggcgc aggccatcag cgtggagacg
     1141 tccaccgagt tcgagaggtt gaagcccacg agcggcgtcg acccgtcctc cctggcgaac
     1201 agcctggcca tcatcagcgt ccagaggaca gcgtaggggt tgtcgttgcc catgaagacg
     1261 gagatcttga agccgatgtc gatgcccagc ctgtggagca ggtagcccag cagcaccgtc
     1321 ccggggttga tgttgagcac ctcgttgatc ccgtagttgg tgtagtagta cagcagctcg
     1381 tccgcccacg ccagggggtg ttcgttgggc tgccctatgc ccccgaagta gcccgtgatg
     1441 gtctccggcc ccccgaggtg gacgttggaa ccgtcggtcc ccttcgtgtc gagggtctcg
     1501 gcgtaactgg cgccgacgat ctgcatggcc gccgcgacgg ccagggtgtc gccctggtcg
     1561 cgctcctgct ccttcatctt ccggaccctt atgtaccgtc cgggcatgat ctccttcctt
     1621 tcgatggcct gttccgcctc ggccaccagg aagggaaagt actggagggc gctgatctcg
     1681 agcgtcactg aagtggactc gctgaaggtc ctgccggcgg gatcgccgcc caataccttt
     1741 ttcctgtact ccgatatggg gatgaaggcc cccttgtccc tctccgactc gagccagttg
     1801 aggtcctcca ggtaggggga gcccatccgc tccaggcgcc ccatgaggct tgaaagacac
     1861 ctggcctccc tcgccttggc gttgatctcc tccggggtac cgtacttcgc gacgaccctc
     1921 aggagggcct gcacatcggg gttgcccggg tcaagcatga agcggttgat ctcctccagc
     1981 gtctcctggc tgatgcgaaa catctcgctc aacatt
//
LOCUS       DAOSRG010000222         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51330_length_2015_cov_8.909694, whole genome shotgun sequence.
ACCESSION   DAOSRG010000222 DAOSRG010000000
VERSION     DAOSRG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51330_length_2015_cov_8.909694"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1978
                     /gene="infB"
                     /locus_tag="PLM30_06390"
     CDS             23..1978
                     /gene="infB"
                     /locus_tag="PLM30_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR54835.1"
                     /translation="MSKIRVYELAKMLGRSNKEMMDLLKDLGIEVSNHMNSIESETAQ
                     LVEDALREGSDRPPREGKPARRVVVPKGALINEVATLLDKKAGELVRILVSSGHMVPA
                     NSPVTDEILQVLSDELGFEIVTSDERAPGESTVVSKKPVFKGDNLQERAPIITVMGHV
                     DHGKTTLLDHIRKTRITETEAGGITQHIGASTVVHKGKRIVFLDTPGHEAFTAMRARG
                     AQATDIVILVVAADDGVKPQTLEALNHAKASGVPIVVAVNKIDKPEARPDRVRQQLSD
                     HGLVPEEWGGDTIAVDISAVTGQGIDQLLEMVLIVADLLELKADPTVSPEGTVIEARL
                     DKGKGPVATVLVQQGTLRKGDIVQTDSAWGRIRAMIDDAGNQIDAAGPSTAVEVLGFS
                     SVPQPGELFSIAPSEKEARQAISDREAARKEAGTTPSRKITLEEMYDRMQMGETPVLS
                     IVLKCDVQGSLEALKASLKKIETDEVGIQFVHGGVGRISESDVTLASASNAIIIGFNV
                     RPDPNARRIAEDEGVQIRLYRIIYDVIEDVHAALEGMLAPTIREHVLGQAEIRAIFKV
                     PRAGKVAGCYVTEGVIKRGAKVRLIRDGVVFWEGGLSALRRFKDDAREVSAGYECGMS
                     FSNQQDFQEGDIVEAFELVEERRTLER"
BASE COUNT          465 a          541 c          657 g          352 t
ORIGIN      
        1 gtggccgggg aggtcgatag aaatgagcaa gatcagggtc tatgaactgg cgaagatgct
       61 gggaagatcc aacaaggaaa tgatggacct gctgaaggac ctcggcattg aggtcagcaa
      121 tcatatgaac tccattgaat ccgagacggc acagctggtg gaggatgccc ttcgcgaagg
      181 gagcgataga cctcccaggg aggggaagcc ggccagaagg gtggtcgtcc ccaagggggc
      241 cctgatcaac gaggtggcga ccctcctgga taaaaaagcc ggcgaactcg tcaggatact
      301 ggtatcctcg ggccacatgg tcccggccaa ttcgcccgtc accgatgaga tcctccaggt
      361 tctctccgac gaacttggtt ttgagatcgt aacgagcgac gagagggccc cgggagagag
      421 cacggtggtg agcaaaaagc ccgtttttaa aggagataac ctccaggaaa gggctcccat
      481 tataaccgtc atgggtcacg tcgaccacgg gaaaacaacc cttctagacc atatcaggaa
      541 aaccaggatc accgagaccg aggcgggagg tatcacgcag catatcggcg cttccacggt
      601 ggtccataag gggaaaagga tcgtcttcct ggacaccccc ggccacgagg ctttcacggc
      661 catgagggcc aggggggccc aggccacgga catcgtgatc ctggtggtcg ccgctgacga
      721 cggcgtcaag ccccagaccc tcgaagccct gaatcacgcg aaggcttcgg gtgtcccgat
      781 cgtggtggcc gtcaacaaga tcgacaaacc cgaggccagg cccgaccggg tccgacagca
      841 attgtcggat cacggcctcg tccccgagga gtggggcggg gacaccatcg ccgtcgatat
      901 ttcggccgtt accggccagg ggatagatca actgttggaa atggtcctga tcgtggccga
      961 cctacttgaa ctgaaggccg accctaccgt ctcacccgaa gggaccgtga tcgaggccag
     1021 gcttgacaag ggcaagggcc ccgttgccac tgttctggtc caacagggca ctttgagaaa
     1081 gggcgatatc gtccagaccg attctgcctg gggaaggatc agggccatga tcgacgacgc
     1141 agggaaccag atcgacgcgg ccggccccag tactgccgtc gaggtgctgg gtttttcgtc
     1201 ggtgccgcaa ccgggtgaat tgttctccat cgctccgagt gaaaaagaag cccgccaggc
     1261 gatcagcgat agggaggccg caagaaaaga ggcgggtact accccgtcga ggaagatcac
     1321 cctcgaggag atgtacgacc ggatgcagat gggggagacg cccgtcctat ccattgtcct
     1381 caaatgcgat gtccagggtt cccttgaagc ccttaaggct tccctgaaga agatcgagac
     1441 cgacgaggtg ggcatccagt tcgtccatgg aggcgtaggg aggatctcgg agtcggatgt
     1501 gacgctggct tccgcctcaa acgccattat catagggttc aacgtcaggc ccgaccccaa
     1561 cgcgcggagg atcgcggagg atgagggggt ccagatcagg ctttacagga ttatctacga
     1621 tgtaatagag gatgtccatg ccgccctcga gggcatgctg gcgccgacca ttcgggagca
     1681 cgttcttggg caggccgaga tccgtgcgat cttcaaggtc ccgagggcgg gtaaggtcgc
     1741 aggctgctac gtgaccgaag gtgtgatcaa gcggggggcg aaggtgaggc tcatcaggga
     1801 tggtgtcgtc ttttgggagg gcggcctctc cgccctcaga agattcaagg atgacgccag
     1861 ggaagtctcg gcgggatatg aatgcggcat gagcttctcg aaccagcagg attttcagga
     1921 gggcgacatc gtagaagcct ttgaactggt cgaggaaagg agaaccctgg aaagatgagg
     1981 gaacgggccg ccttctgcgg catgctgttc gttca
//
LOCUS       DAOSRG010000223         2000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_51884_length_2000_cov_7.615424, whole genome shotgun sequence.
ACCESSION   DAOSRG010000223 DAOSRG010000000
VERSION     DAOSRG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51884_length_2000_cov_7.615424"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1801
                     /locus_tag="PLM30_06395"
     CDS             65..1801
                     /locus_tag="PLM30_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOR54836.1"
                     /translation="MEGKGSAKSAAVALLPVILIAAMTSVSSAATRAEVIDVIVSSLG
                     LPVWAGEKHFSDIGPDHPFGRSVETASALGILDPAERFYPDMEATRAEVVMFALQSMG
                     LRHEAWLTNSLAPGSWPNLPVFITPYMTLASQIQPSPPREFLLQPREGVSQKDLFSLE
                     TWLRECSLGLSWQKEFKGEDSVLVLRRDHVGRPPSCWGVRSVEFEEPEEAERAASILS
                     KTGVMATVHRLEWTWVVRVGPFQHYMEAWETMMKIPAPEMTVAPFGDDPGRALFIAAL
                     GFDPAVSPPRIVTAASISGKRLPLGIIAENSGARGAINGGFFSGTRIVGSLVTGSKPL
                     SGPHGARSAMGWSRDGLKVHFGRGDFKTFLSFGGKELAVAGMNNIPPLGGIGVFTPDV
                     WSYITGVPADGWEVTVKGGIVTGVRHASTSNHFVTREGFLVIGRGAAGELIKDIQPGT
                     PLTLRIQWVDDGFTGLDNILQAGPMLVKGGEKVFDPEGFSPRTLSVPHPRSFVGSDGE
                     RLWFVVIDGRDPWHSNGASIAETAAAAQRLGLKNALNLDGGGSSSIWWSGKIVNFPPG
                     GVVRPVPYALVF"
     gene            complement(1783..>2000)
                     /locus_tag="PLM30_06400"
     CDS             complement(1783..>2000)
                     /locus_tag="PLM30_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR54837.1"
                     /translation="VQGILSFDERAVSLRALIRTVPGEHWTIGMEYRLRLKEAFDAEG
                     ITIPFPQMDVWLRSPEGLRVAGQKTRA"
BASE COUNT          370 a          577 c          666 g          387 t
ORIGIN      
        1 taggtgaggg aaagcaattt cccggcaccg ttgtgctttt ttcaggggtg gggggatgac
       61 gggaatggaa gggaaagggt cggcaaaaag cgcggcagta gcgttgctcc cggtcatact
      121 gatcgctgcc atgacctcag tttcatcggc ggccaccagg gcggaagtga tcgacgttat
      181 agtctcttcc ctaggccttc ccgtatgggc gggcgagaaa catttttccg atatcgggcc
      241 tgatcatccc ttcggccgct ccgtggagac ggcgtcggcc ctgggcattc tcgatccggc
      301 tgagcgcttt taccccgata tggaggcgac cagggccgag gtggtgatgt tcgccctcca
      361 atcgatgggc ctccgccacg aggcctggtt aacgaactcc cttgcaccgg ggagctggcc
      421 gaacctcccg gtcttcatca cgccctacat gaccctggcc tcgcagatcc aaccttcgcc
      481 ccctcgagag ttcctgctgc agccgcggga aggggtatcg caaaaggacc tcttctcgtt
      541 ggagacctgg ctgagggagt gctccctggg gctatcctgg caaaaggagt tcaaaggaga
      601 ggattccgtc cttgtcctcc ggcgcgacca tgttggaaga cccccatcgt gctggggggt
      661 ccgatcggtc gagttcgagg aacccgaaga ggccgaaaga gcggcgagta tcctcagtaa
      721 gaccggggtt atggcgacgg tgcacaggct ggagtggacc tgggtcgtga gggtcgggcc
      781 cttccagcat tacatggaag cctgggagac catgatgaag atacccgcgc ctgaaatgac
      841 ggtagcccct ttcggtgacg acccgggcag ggcgctcttt atcgctgccc tgggtttcga
      901 ccccgctgta tcaccaccga ggatagtgac cgccgcctcc atatcgggaa agcggcttcc
      961 cctggggata atagccgaga attccggtgc acggggggcc ataaacggcg gttttttcag
     1021 cgggaccagg atcgtcggtt ccctggtgac ggggtccaag ccgctgagcg ggccccacgg
     1081 cgccaggtcg gccatggggt ggagccggga cggcctcaag gtccatttcg ggagggggga
     1141 tttcaaaacc tttctttcct tcggcgggaa ggagctcgct gtggctggca tgaacaatat
     1201 ccccccgctg ggcgggatag gggtgttcac ccccgacgtc tggagctaca tcaccggcgt
     1261 ccccgccgac ggatgggagg tgaccgtgaa ggggggcatt gtcacgggtg tcaggcacgc
     1321 ctccacgtca aaccactttg tgaccaggga gggcttcctg gtcataggcc gcggggccgc
     1381 gggggaactt atcaaggata tccagccggg gactcccctg accctcagga tccaatgggt
     1441 cgacgatgga ttcacggggc tcgataatat actgcaggcg gggcccatgc tggtgaaggg
     1501 cggggaaaag gtctttgacc cggagggttt cagcccgagg acgttgtcgg tgcctcaccc
     1561 gagatccttt gtgggcagcg acggtgaaag gctctggttc gtggtcatcg acggaaggga
     1621 cccctggcac agcaacggtg cgtctatagc cgaaacggcg gcggccgccc aaaggctcgg
     1681 ccttaaaaac gccctgaacc tggatggtgg gggctcctca tcgatctggt ggtcgggaaa
     1741 gatcgtcaac ttcccgcccg gaggagttgt gcggcctgtc ccttacgccc ttgtcttctg
     1801 accggccacc ctgagccctt cgggggaacg gagccagaca tccatctgtg ggaagggtat
     1861 cgtgatgccc tcggcgtcga aggcctcctt cagacgcaga cggtattcca taccgatggt
     1921 ccagtgttca cccgggacgg tccttatgag ggcccttagg gagacggcgc gctcatcgaa
     1981 ggagaggatc ccctggaccc
//
LOCUS       DAOSRG010000224         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_52077_length_1995_cov_8.362371, whole genome shotgun sequence.
ACCESSION   DAOSRG010000224 DAOSRG010000000
VERSION     DAOSRG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52077_length_1995_cov_8.362371"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..920)
                     /locus_tag="PLM30_06405"
     CDS             complement(138..920)
                     /locus_tag="PLM30_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOR54838.1"
                     /translation="MLLPATCVPSAAEELPWEEHPVRQGETLDTIAEQYPVDSNCIRR
                     ANELSGDVPPEGLTLLVPHNDGLLLETLAEVRARRKGETTADRYIEEDDRPGQAPMPA
                     SPRVDRMRSEYPGIEGLIALARGSAAEAAVITRPQLSWPVEGIVFSPFGLRRGRFHCG
                     IDISAPRGTPIRTAAPGTVVRAATRRGFGKSILIDHGGGVMTRYSHCDTMLCKAGDKV
                     SPGQVIGKVGRTGRTTGPHLHFEVVIDGKHQDPQKHLPQRGD"
     gene            complement(1007..>1995)
                     /locus_tag="PLM30_06410"
     CDS             complement(1007..>1995)
                     /locus_tag="PLM30_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR54839.1"
                     /translation="RARDSLILCGASPRTEEGELRSKRHSWLETVLDWAESDGSGPPE
                     PEPPTEGAARRGKAAPATKGERVDLPPEEGGTLERISATAYALLRFCPFAFRMRHRQG
                     LDISWEMPSDDVGGADLGSLAHWLLRRWDLRPETLSLYDPLRDGAGLGELLPADLRPV
                     WADPAKRVPLMGWLERFASSPLAERIRNGEDTRKEVPFRVRLDNGALMVGVIDVIWRE
                     GEKTCIRDYKIGRIENAPGELYRSQLLFYALAARKHFGGSPLDLALLSLKENREIPVG
                     KDPFSWEDLEEDITSAARQGAAGPFEPSRKMCPLCPWKGECVLGREWTKGGL"
BASE COUNT          310 a          658 c          642 g          385 t
ORIGIN      
        1 ctggtattgt ccttggggta aaagtagagg accacccact tgccggtgaa ctcctcgaga
       61 catctctccc tgcccgtgct gtccttcagg cagaagggcg gtgcctttgt tccttcttcc
      121 accatggcat atccctccta gtctccccgc tggggcaaat gcttttgagg atcctggtgc
      181 ttcccgtcga tgaccacttc gaagtggagg tgggggcccg tggtcctgcc cgtcctgccc
      241 accttcccga tgacctggcc cggggagacc ttgtcaccgg ccttgcagag catggtgtcg
      301 cagtgggaat aacgggtcat caccccgccg ccgtggtcga tgaggatgga cttgccgaag
      361 cccctcctcg tggctgccct gacgacggtc cccggtgccg ccgtccggat gggagtaccc
      421 ctcggggccg atatatcgat gccgcagtgg aacctcccac gtctaaggcc gaagggcgaa
      481 aagacgatcc cttcgacggg ccatgagagt tgggggcggg tgatgacggc agcttcggcg
      541 gccgatcccc tcgcgagggc gatgaggcct tcgatacccg ggtattccga tcgcattcta
      601 tctacccgcg gtgatgccgg catcggggcc tgcccgggac ggtcatcctc ttcgatgtaa
      661 cggtcggcgg tggtctcccc cttgcgcctg gcgcggacct cggccagggt ctcgagcaac
      721 agaccgtcgt tatggggcac cagcagggtc agcccctccg gggggacatc tccgctgagt
      781 tcgttggccc gccgtatgca gttactatcg acggggtact gttccgctat agtgtccagg
      841 gtctcgcctt gtcggaccgg gtgctcctcc caggggagtt cctcggccgc cgatgggacg
      901 caggtcgccg gcagcagcat gacagctaac gccaggacgg ctcgggcaaa aaccatggaa
      961 tggtcatccc cccgtcagag tccggtttgg gtacggggca atgatatcat aagccccctt
     1021 ttgtccattc ccgccccagt acgcattcgc ctttccaggg gcacagggga cacatctttc
     1081 gggagggctc gaagggtcca gccgcacctt ggcgggccgc cgaagtaatg tcctcttcga
     1141 ggtcctccca ggaaaagggg tccttcccca cggggatctc ccggttctcc ttcagcgaca
     1201 ggagggccag gtccagcggt gacccgccga agtgcttcct ggcggcgagg gcgtaaaaga
     1261 gcagctggga ccggtagagt tcccccggcg cgttttcgat ccggcctatc ttgtaatccc
     1321 ttatacaggt tttttcgccc tccctccaga tgacgtcgat gacccccacc atgagggcgc
     1381 cgttgtcgag gcgcaccctg aaggggactt ccttccgggt gtcttccccg ttccttatcc
     1441 tttcagccag cggggaggag gcgaaacgct cgagccagcc catgaggggg actcttttcg
     1501 ccgggtcggc ccagaccggc ctaagatcgg cggggagaag ttcccccagg ccggccccgt
     1561 cccggagggg gtcgtaaagg gagagcgttt ccggacggag gtcccaccgg cgcaacagcc
     1621 agtgggccag gctccccagg tcggccccgc ccacgtcatc ggagggcatc tcccaggaga
     1681 tgtccaggcc ctgcctgtgc ctcatcctga aggcgaaggg acaaaatctg agtagggcgt
     1741 aggccgtggc gctgatcctc tccagggtcc cgccttcctc cgggggaagg tcgacccttt
     1801 cacccttcgt cgctggggcg gccttacccc gccgggcggc cccttcggtg gggggctccg
     1861 gctcgggcgg gccggagccg tcgctttcgg cccagtccag cacggtctcc agccaggaat
     1921 gcctcttcga gcggagctct ccttcctcgg tccgggggga ggccccgcag aggatgagcg
     1981 agtccctcgc cctgg
//
LOCUS       DAOSRG010000225         1986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_52439_length_1986_cov_9.305541, whole genome shotgun sequence.
ACCESSION   DAOSRG010000225 DAOSRG010000000
VERSION     DAOSRG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52439_length_1986_cov_9.305541"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLM30_06415"
     CDS             <1..1097
                     /locus_tag="PLM30_06415"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOR54840.1"
                     /translation="WKEKMPAPVRDRTFAEYGSSREERPVLVDCALGTNPLGSPPWAK
                     EYFESGNLPDPGGYPGDDLPLRKALSDSWNGAFSPDEIVTGTGSIGLIISLAGAFCAP
                     GAEVLGVAPQFPDGPMHFQFAGASYRFLPLTPPEFDLDLSRLLGEMTGEESIVYVDRP
                     HNPTGQVPPLTEMARLAEECREKGSLLVIDEAYGDFLPPEESALNLTSPSVVVLRSFS
                     KGRGLAGLRAGYCVIRNEEARKYLRKVAPPFSVGSLAMEMARASLLDAGYVARSRETV
                     RDVKARILQVLEGTRGITVARTDPEVPILLATWGEGSLDLYEALMEQGIRTEPGTCFP
                     GIGPESVRLRVPAPEELETFERLWKRTFEE"
     gene            1462..>1986
                     /locus_tag="PLM30_06420"
     CDS             1462..>1986
                     /locus_tag="PLM30_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54841.1"
                     /translation="MSDPVVNRGFFGKMMGSSFYKKFILPGLISQSVVIAGGYGTGRE
                     LVEYFGNFGSLGGILGMVLVTTVLWAIVYAVSFEFARTFKVYDYRSFFKELIGPGWML
                     YEVCYIILLLIILGVVGATSGSVFKQAFGLPPLVGAGLFLAGVATLTFFGSYVIEVAL
                     SWWSFVLYAVFLIFL"
BASE COUNT          340 a          632 c          606 g          408 t
ORIGIN      
        1 agtggaaaga gaaaatgccg gcgcccgtga gggacaggac cttcgccgag tacggatcct
       61 ccagggagga gaggcccgtc ctggtggact gcgccctggg gaccaacccc ctgggctccc
      121 ctccctgggc taaggagtac tttgagtcag ggaaccttcc cgacccgggc ggctatcccg
      181 gcgacgacct ccccctgcgg aaggccctgt ccgactcctg gaacggcgcc ttttcccccg
      241 acgagatcgt caccggcacg ggctccatcg gcctgatcat cagcctggct ggagcctttt
      301 gcgccccggg tgccgaagtc ctaggggtcg cgccccagtt ccccgacggg cccatgcact
      361 tccagttcgc cggggcgtcc taccggttcc tgccgctcac acccccggag ttcgacctcg
      421 acctctcccg cctcctggga gagatgaccg gcgaggaatc catagtatat gtcgatcgtc
      481 cccacaaccc caccgggcag gtccctcccc tgaccgagat ggctcgcctg gccgaggagt
      541 gccgggaaaa gggctcgctc ctggtcatcg acgaagccta cggcgatttc ctcccgcccg
      601 aggagtcggc cctcaacctg acctcgccct cggtggtggt cctccgctcc ttctccaagg
      661 gccgcggcct tgccgggctg cgcgccggct actgcgttat ccgcaacgag gaggccagga
      721 aatacctccg gaaggtggct ccccccttct cggtcggctc cctggcgatg gagatggcca
      781 gggcgtccct cctcgacgcc ggttatgtgg cccgctcgag ggagaccgtg agggacgtca
      841 aggccaggat cctccaggtc ctggagggga ccaggggcat caccgtagca aggacggacc
      901 cggaagtgcc catcctcctt gccacctggg gggaggggtc gctggacctc tacgaggccc
      961 tgatggaaca gggcatccgg accgagccgg ggacctgctt tccgggcatc ggccccgaga
     1021 gcgtccggct gagggtcccg gctcctgaag aactggagac ttttgaaaga ctctggaaga
     1081 ggacctttga agaataaagg ctggccggac ctggtacggc gtttgacaag tgcgggtcgc
     1141 tggtatagac tcccatagat actacgtttt ttaataatcg atcgccttga agggagggtg
     1201 acccgtgctg agagagaatg accttttggg ctgctatttt tacggctatt acttttactt
     1261 cgccttgggg tccccctcct ggcgcggttg agccgtactc ttccgcaacg aaccgggata
     1321 aagggacccc ttcaaaaggg gtccctttct tttttggggg tgatgccaag ggacatcctg
     1381 gaagaaagac acccgcgggc atttaaggga cgccctgtaa ctgggcgggc agacaaccag
     1441 acacaggagg tgtcgaacga aatgagcgac cccgttgtga accggggttt tttcggcaaa
     1501 atgatgggtt cttccttcta caagaagttc atccttcccg gccttatttc ccagtccgtc
     1561 gtcatcgccg ggggttacgg cacaggtcgt gaactcgtcg agtacttcgg gaatttcggc
     1621 agcctcggcg gcattcttgg catggtgctg gtaaccacgg tcctgtgggc catcgtctac
     1681 gccgtgagtt ttgagttcgc caggaccttc aaggtttacg actaccggag ctttttcaag
     1741 gaactcatag gacccggctg gatgctctac gaggtctgct acataatcct cctcctcatc
     1801 atcctcggcg tcgtcggagc cacttcgggg agcgtcttca agcaagcctt cgggctgccg
     1861 cccctggtgg gggccggcct cttcctggcg ggcgtcgcca cgctgacctt cttcggcagc
     1921 tacgtcatcg aggtcgccct gtcgtggtgg tccttcgtcc tctacgcggt cttcctgatc
     1981 ttcctc
//
LOCUS       DAOSRG010000226         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_53259_length_1964_cov_8.832373, whole genome shotgun sequence.
ACCESSION   DAOSRG010000226 DAOSRG010000000
VERSION     DAOSRG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53259_length_1964_cov_8.832373"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLM30_06425"
     CDS             <1..539
                     /locus_tag="PLM30_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOR54842.1"
                     /translation="TGGERIFLERGVTGIRGEAEGGFPTVLRHGLPSFRKALSRGAAR
                     HDSALYALFDLMMHGEDTNVIARKGYSFWRSDYRRLVGGLLDLGTPPYDDNALASLKE
                     ADEQFRLQDISPGGAADLLTCTLFLHDCDTLPEDIVNIQSDRYNMCNITYGGADHEPG
                     IEDQDPSQVEGAHPEKTL"
     gene            472..828
                     /locus_tag="PLM30_06430"
     CDS             472..828
                     /locus_tag="PLM30_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR54843.1"
                     /translation="MSLGLRIKTLRRSKGLTQKKLSEMVDVSRIYIQSLESNRRLPSM
                     KLLKRLAAALDVEVVDLVQNPGRDGSGRLMLEEVLNDSEPVEIWYRSRKLSRGEVQLV
                     QKLIEAALSDWDREND"
     gene            821..1246
                     /locus_tag="PLM30_06435"
     CDS             821..1246
                     /locus_tag="PLM30_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOR54844.1"
                     /translation="MTKTALDGKTEEGFLWPEPPEFIPEWASLEARQWKDLDDFDLLA
                     KVEAACGRSVEIVRAPLPVSLWGLHVARGRRARIYVNGLLPEIWRRFALFHEIYHLTH
                     HARGEDFWSHTATPMTSFEYQADLFAWAAIIDEWGEGAS"
     gene            1246..>1964
                     /locus_tag="PLM30_06440"
     CDS             1246..>1964
                     /locus_tag="PLM30_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR54845.1"
                     /translation="MERAPFFLPMRDCPNRCVYCDQRAITGHAGSPEPRDVREALQAI
                     AEPTEVCFFGGSFTCQPLGRQRDYLEAALAAPSPVNFRISTHPLCVDPSILAFLSHFP
                     VRIIELGVSSLEDEVLETCKRGYTGAEVLERISLVAANPAFIPGAQLMTGLPGQTPSS
                     SLEDLRRLAAVKGKGPMQIRIYPCLVLKGTLLERWYLSGDYTPPGIEESARWAGRMIK
                     YAREAGFELLRVGLQETVSLSG"
BASE COUNT          383 a          628 c          597 g          356 t
ORIGIN      
        1 tgacgggagg agagagaata ttcctggagc gcggtgtcac cggcatcagg ggcgaagccg
       61 agggaggctt ccccaccgtc ttgaggcacg gtttgccttc cttcaggaag gccctctccc
      121 gcggggctgc caggcacgac agcgcccttt acgccctttt cgacctcatg atgcacgggg
      181 aggacacgaa cgtcatcgcc cggaaggggt attccttctg gaggagcgac taccggcgcc
      241 ttgtgggcgg gctcctggac ctgggaacgc ccccctacga cgacaacgcc ctggcctcac
      301 tcaaggaagc cgacgaacaa ttccgcctcc aggatatcag ccccggcggg gccgcggacc
      361 ttttgacctg caccctgttc cttcacgact gcgataccct tccggaggac attgtaaaca
      421 ttcagagtga caggtataat atgtgtaaca ttacgtacgg aggtgcggac catgagcctg
      481 ggattgagga tcaagaccct tcgcaggtcg aaggggctca cccagaaaaa actctctgaa
      541 atggtcgatg tcagccgcat ttatatccaa tccctggaga gcaaccggcg gctgccctcg
      601 atgaagctcc tcaagcggct ggccgcggcc ctcgatgtag aagtggtcga cctggtccag
      661 aaccctggaa gggacggttc gggcaggctc atgctcgaag aggtgctcaa cgatagtgaa
      721 cccgtcgaaa tatggtaccg gagcaggaag ctctccaggg gagaggtgca acttgtacag
      781 aagctcatcg aagcggcgct ctccgattgg gacagggaga atgactaaaa cggccctgga
      841 cgggaagacg gaggaggggt tcctatggcc cgagccgccc gagttcatcc cggaatgggc
      901 ttcccttgag gcccgtcagt ggaaagacct tgacgacttt gacctcctcg cgaaagttga
      961 agccgcctgc ggcaggtccg tcgagatagt aagggccccg ttgcccgtct ccctatgggg
     1021 tctccacgtc gccagagggc ggagggcgcg catctacgtg aacggcctcc tcccggaaat
     1081 atggaggcgt ttcgccctct tccacgagat ctaccacctc acccaccacg ccaggggcga
     1141 ggacttctgg tcccacaccg ccaccccgat gacgagtttt gaataccagg ccgacctgtt
     1201 cgcctgggcc gccataatcg acgaatgggg cgaaggtgcc agctgatgga acgcgccccc
     1261 ttcttcctcc ccatgaggga ctgccccaac cggtgcgttt actgcgacca gagggccata
     1321 accggtcatg ccggctcccc ggaaccccgg gacgtgaggg aggcgctgca ggcgatcgcc
     1381 gaacccaccg aggtctgctt cttcggcggc agcttcacct gccagccgct gggccggcag
     1441 agggattacc tagaggcggc cctggccgcc ccctccccgg tcaacttcag gatctccacc
     1501 catcccctct gcgtcgaccc gtcgatactg gcctttcttt cccatttccc ggtcaggata
     1561 attgaactgg gtgtttcctc cctcgaggac gaggtgctgg agacttgcaa aagggggtac
     1621 accggggccg aggtcctgga aaggatctct ctggtagccg cgaacccggc tttcatcccc
     1681 ggggcacagc tgatgaccgg cctccccggg caaacccctt cgtcgtccct tgaggacctg
     1741 cggcgccttg ccgccgttaa gggcaagggt cccatgcaga tcaggatcta cccctgcctc
     1801 gtcctgaagg gcactctcct cgaaagatgg tacctctcag gcgactacac cccgcccggc
     1861 atcgaggaga gcgcccgttg ggcgggtagg atgatcaagt acgcccggga ggccggtttc
     1921 gaactcctgc gggtgggcct ccaggagacg gtctccctct ccgg
//
LOCUS       DAOSRG010000227         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_54167_length_1941_cov_8.620891, whole genome shotgun sequence.
ACCESSION   DAOSRG010000227 DAOSRG010000000
VERSION     DAOSRG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54167_length_1941_cov_8.620891"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLM30_06445"
     CDS             <1..467
                     /locus_tag="PLM30_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOR54846.1"
                     /translation="DWVDDKRRDESVRCNQILAVSLPASVMDPERGRSIVETCWNELY
                     TTFGLRTLDPHHDKYKGRCEGRIDQREKARFMGMAWPWLLGQFITAYLRYNPDRKDIG
                     WCFLRPFLSHLRKGCLGGVAEIFDGSMPYKPHGDALYSPSVSELLRVIGEDM"
     gene            561..1577
                     /gene="gap"
                     /locus_tag="PLM30_06450"
     CDS             561..1577
                     /gene="gap"
                     /locus_tag="PLM30_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOR54847.1"
                     /translation="MIKKRVAINGFGRIGRLALRALFEQQEDVLFDIVATNSRTTAEQ
                     RAYLFKYDSVHRRFSGDVSTQGDSLLINGKSVQVLAEPELENLPWKDLGIDLVIESSG
                     KYTDAAKAAKHLLAGARKVLITAPAKNEDATIVMGVNEEIYEPGKHHVVSNASCTTNC
                     LAPVVKVLHEAFGIEKGLMTTTHAYTNDQKTLDASHKNLHRGRAAALSMIPTTTGAAS
                     AIGKVYPAVAGKMNGLAVRVPTPDVSLVDLVTVLSREVTSEEVNAAFREAAEGPMQRY
                     LGYEEEDIVSVDVTGDSRSSLFAARHTMALGNLVKTLSWYDNEWGYACRVIDLVNYMF
                     SKGS"
     gene            1640..>1941
                     /gene="pgk"
                     /locus_tag="PLM30_06455"
     CDS             1640..>1941
                     /gene="pgk"
                     /locus_tag="PLM30_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOR54848.1"
                     /translation="MDLNVPMEGGKVTDDTRIRAHLDTLGILRQGGARVALASHLGRP
                     KGKVAADLSLFPVSQALGALLGCEVPFCEDCVGEKVSARVSSMGDGDVCLLENLRF"
BASE COUNT          439 a          550 c          569 g          383 t
ORIGIN      
        1 tcgactgggt ggatgataag agaagggacg aatcggtgcg gtgcaaccag atccttgccg
       61 tgtcgctgcc agcctccgtc atggaccccg aaaggggaag atcgatcgtt gaaacttgct
      121 ggaacgaact ttacaccacc tttggtctca ggaccctcga ccctcaccat gataagtaca
      181 agggccgatg cgaggggagg atcgaccaga gggaaaaggc ccggttcatg gggatggcct
      241 ggccctggct tttgggccag ttcataacag cctaccttag gtacaacccc gacagaaagg
      301 atatcgggtg gtgcttcctc cgccccttcc tctcgcatct aaggaaaggg tgtctcggcg
      361 gggtggcgga gatctttgac ggcagtatgc cttacaaacc ccatggcgat gccctttatt
      421 ccccgagcgt ctcggaactg ttgagggtca taggggagga catgtgacgg tatttgcacc
      481 tgccctccat ccactggacc tggcgatcga ggttcttttt ctgttgcgga atagaaaaaa
      541 taatacggga ggcgatgaaa atgataaaaa aacgtgtcgc tattaacggt tttggacgga
      601 tcgggcggct ggccctcagg gcccttttcg aacaacaaga ggatgtcctt ttcgatatcg
      661 tggccacgaa cagccggacc acggcggagc agagggctta cctattcaag tatgattccg
      721 tccatcggcg tttttccggc gatgtctcaa cccagggtga ttcactcctt atcaatggga
      781 agtcagtcca ggtcctggcg gaacccgaat tggaaaacct gccctggaag gacctgggga
      841 ttgacctcgt aatagaatct tccggcaagt acaccgacgc tgccaaagcc gccaaacacc
      901 ttttagccgg cgccaggaag gtcctcatca ccgcccctgc aaagaacgag gacgcgacga
      961 tcgtcatggg cgtgaacgag gagatctacg aacccggcaa gcatcacgtg gtctccaacg
     1021 cctcctgcac aacgaactgc cttgccccgg tggtcaaggt gctccacgag gccttcggca
     1081 tagaaaaggg tctcatgacc accacccacg cctataccaa tgaccagaaa accctcgacg
     1141 cctcccacaa gaaccttcac agggggaggg cggccgccct atcgatgatc cccaccacga
     1201 cgggagcggc ttccgcgatc gggaaggttt acccggcggt ggcgggcaag atgaacggct
     1261 tggccgtcag ggtcccgacg cccgacgtct ccctcgtcga cctcgtgacg gtactatcca
     1321 gggaagtgac ctccgaggag gtcaacgccg ccttccgcga agcggcggaa ggaccgatgc
     1381 aacgctacct tggctacgaa gaggaggaca tcgtctccgt cgacgtcacg ggagacagcc
     1441 gctcctcact gttcgcggcg aggcatacca tggccctggg caacctggtc aagacccttt
     1501 catggtacga caacgaatgg ggatacgcct gccgtgtcat agacctcgta aattacatgt
     1561 tttcaaaggg atcctgatgg tcatgaggtt gaaaaccatc gaggactgct cctttcgggg
     1621 gaaaaacgtc cttgtccgag tggatctcaa tgtacccatg gaaggcggca aggtcaccga
     1681 tgataccaga ataagagccc acctcgacac tttaggtatc ctcaggcaag gaggggcaag
     1741 ggtcgccctt gcctcccacc tgggaaggcc gaagggaaag gtcgcggccg atctatccct
     1801 ttttcccgtc tcccaggccc tgggggcact gctgggctgc gaggtcccct tttgcgagga
     1861 ctgcgtgggg gagaaggtct cggcaagggt cagttcgatg ggcgatggcg atgtctgcct
     1921 cctggagaac ttgaggtttt a
//
LOCUS       DAOSRG010000228         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_54280_length_1938_cov_6.953266, whole genome shotgun sequence.
ACCESSION   DAOSRG010000228 DAOSRG010000000
VERSION     DAOSRG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54280_length_1938_cov_6.953266"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLM30_06460"
     CDS             complement(<1..716)
                     /locus_tag="PLM30_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR54849.1"
                     /translation="MREITFSQATLEAMDEEMTRDERVFVMGEDIARQGGIFGQFKGL
                     PDKFGKDRVRDTPISETAIVGAAIGAALTGMRPVADMHFADFMGVCMDEIFNQMAKIH
                     YMFGGQRPVPMVLRAPDGLINQAAAQHSQSIEAWFMHIPGLKVVIPSNPADAKGLLKS
                     AIRDDNPVIYFEHKALFNTRGPVPDGEVLTPIGKAKVVREGSDVTLVSYSRCMGLSLE
                     AADVLAGEGISVECIDLRSIS"
     gene            complement(738..1736)
                     /locus_tag="PLM30_06465"
     CDS             complement(738..1736)
                     /locus_tag="PLM30_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOR54850.1"
                     /translation="MSARKVKEKKGPDKYPKALLAQFYKTMSTIRSFEKKVEEKFLAG
                     EIPGFVHLYIGEEAVATGVISNLSREDLIQSTHRGHGHTIAKGADLKRMMSEIFGKKE
                     GCCKGKGGSMHIADLGVGMLGANGVVGGGFNIATGAALAVKMQGKDNVSVVFFGDGAS
                     NRGTFHEAANMAAVWDLPVLFVCENNQYASTTPYRTTTSVDDIADRAAGYGMPGVIVD
                     GNDVFAVYEGARKLIERARRGEGPALLECKTYRVKGHFVGDPEQYRTKEEVNKVCESN
                     DPIDRFECTVLDGKLFSREELDGIKASAVKEVEEALEFARNAAEPEVSQLFADVYA"
BASE COUNT          343 a          648 c          525 g          422 t
ORIGIN      
        1 gaaatgctcc tcaggtctat gcactccacc gatattcctt ccccggcaag gacgtccgcc
       61 gcctccaggg aaaggcccat gcaacgggag taggacacga gggtcacatc gctcccctcc
      121 ctcaccacct tcgccttgcc tatgggcgtc aggacctcgc cgtcagggac gggcccccgg
      181 gtgttgaaaa gagccttatg ctcgaagtag atcacgggat tgtcgtccct gatcgccgat
      241 ttgagaagtc ccttcgcgtc ggcagggttg gaggggatga ccaccttcaa gcccgggatg
      301 tgcatgaacc aggcctcaat gctctgggag tgctgggccg ctgcctggtt tatgaggccg
      361 tccggcgccc ttaacaccat ggggaccggc ctctggccgc cgaacatgta gtggatcttg
      421 gccatctgat tgaatatctc gtccatgcaa acccccatga aatcggcgaa gtgcatgtcc
      481 gccacgggtc tcatacctgt caatgcggcc cctatggcgg cgccgacgat ggccgtctcg
      541 cttatggggg tgtccctcac cctgtccttc ccgaacttgt cggggagccc cttgaactgg
      601 ccgaagatgc caccctgcct ggcgatatcc tcacccatga cgaagaccct ctcgtcacgg
      661 gtcatctcct cgtccatcgc ttccagcgtg gcctgtgaga atgtgatctc tctcatgatc
      721 ctgtcaccaa cccatcctta cgcgtagacg tccgcgaaga gctgcgatac ttccggctcc
      781 gcggcgttcc tggcgaattc gagggcctct tcgacttcct tcacggccga ggcctttatc
      841 ccgtccagtt cctccctgct gaagagcttc ccatccagga ccgtacactc gaaccggtcg
      901 atggggtcgt tgctctcgca gaccttgttc acctcttcct tcgtcctgta ttgctccggg
      961 tcccccacga agtgtccctt cacccggtag gtcttgcact ccaggagagc cgggccctca
     1021 ccccttctcg cccgctcgat cagcttccgc gccccctcgt acacggcgaa cacgtcgttg
     1081 ccgtccacta tcaccccggg catgccgtac ccggccgccc tgtcggctat gtcatcgacg
     1141 gaggtagtgg tcctgtaagg agtggtggag gcgtactggt tgttctcgca gacgaacagc
     1201 acgggcaggt cccagacggc ggccatgttg gctgcctcgt ggaaggtgcc gcggttggag
     1261 gccccgtcac cgaaaaatac gaccgaaacg ttgtccttgc cctgcatctt gacggccagg
     1321 gccgccccgg tagcgatgtt gaaaccgccc ccgacgacac cgttggctcc cagcatgcct
     1381 acgcccaggt cggcgatatg catggagccg cctttcccct tgcaacaccc ttccttcttg
     1441 ccgaagatct cggacatcat cctcttgaga tcggcgcctt tggctatggt atggccgtgc
     1501 ccgcgatggg tgctctggat gagatcctcc cttgagaggt tcgagatcac ccccgtcgcg
     1561 acggcctcct cgccgatgta aagatgaacg aagcccggga tctcaccggc caggaacttt
     1621 tcctccacct ttttttcgaa agacctgatg gtgctcatgg tcttgtagaa ctgggccaga
     1681 agcgccttgg ggtacttgtc cgggcccttc ttttccttaa ctttccttgc gctcaaggat
     1741 ccccacctca tttcgggtaa tgatagcctg gtcccctcgg ccaggcacaa ctcgattgtc
     1801 ccggttattt gctaacgcat cagaagtttc ggaagccaaa gggtgatgcc cggcgcgtag
     1861 acgatcaccg ccaaagccgc gaccgaagcc aggaggaagg gcaccaccgc cctcgatatc
     1921 tcatccatgg agataccc
//
LOCUS       DAOSRG010000229         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_54402_length_1935_cov_8.352660, whole genome shotgun sequence.
ACCESSION   DAOSRG010000229 DAOSRG010000000
VERSION     DAOSRG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54402_length_1935_cov_8.352660"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLM30_06470"
     CDS             complement(<1..289)
                     /locus_tag="PLM30_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOR54851.1"
                     /translation="MRLELHKVRIGNVSWGEATEVRGGTLFINREEMLAVAREEPRFA
                     RVELELARPGESVRIIPVKDIIEPRCKLSGPGEVFPGFVGGTCTVGEGSTLV"
     gene            468..1646
                     /locus_tag="PLM30_06475"
     CDS             468..1646
                     /locus_tag="PLM30_06475"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HOR54852.1"
                     /translation="MLKRFAQEICDITNGIIGHHVLITDENGVTLGSSDLSRLGTVHT
                     PSLECMRSRAETFTDSEEASRLEGVKPGVTLPVCLAGEVVGSIAIAGDHREVARYGYL
                     VQKQAELFLREQMIQESAGARDRAVQELISYIASFAPGHDDPGILAPRIRELGFEPGI
                     PRVCLVLEPGLAKPGAAGKGLDPLERVSLFKEIHEIILSVFPEPLNFGASLGGARFAI
                     FAAIKEVGGGSVLDHAVSGCQRCREKMKGMGLILTVGAGSPAHVLEELHYSYLDAWKA
                     LTIGLKKNDPGGTHSIDDYRLEDLLTTVNMKKGRLFADKTLSGLRKAPDGEEMLRTFH
                     SWCRHPCSPGLVAKDLNVHRNTLGYRIEKIKRLTGLDIRDLGEAFELFLAATLDTLLD
                     "
     gene            complement(1730..>1935)
                     /locus_tag="PLM30_06480"
     CDS             complement(1730..>1935)
                     /locus_tag="PLM30_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOR54853.1"
                     /translation="YEVIYKDPDMRDMDSSVVWVARKLNFPIKGVSEGKDGTVIVTYK
                     NIKEEAIDRSLFEIPKDYNKFSF"
BASE COUNT          426 a          531 c          534 g          444 t
ORIGIN      
        1 ggaccagggt cgaaccctct cccacggtac aggtgccacc cacaaagccc gggaacacct
       61 ccccggggcc ggaaagcttg cacctgggtt cgatgatgtc ctttacaggg atgatcctga
      121 cactctcgcc ggggcgggcg agttcaagtt ccacgcgggc gaaccggggt tcttccctgg
      181 cgacagcgag catctcttcc cggttgatga acaaggttcc acccctgacc tctgtcgctt
      241 ccccccatga gacatttcca atcctgactt tgtgcaattc caacctcatg ggccattccc
      301 tccttacgcg ggatgctatt actttcaaat agaattatag tatcctggcc gacttcagta
      361 tattgttcac ccttaaagac ttgtgccctt cggacagtcc attttttgga cttttgcaca
      421 aaaacttctt ggtccataat ggctttatct tcgaggaggg gatacgaatg ttgaagcgtt
      481 tcgcccagga gatttgcgat ataaccaatg gcatcatcgg gcaccacgtc ctgataaccg
      541 acgaaaacgg cgtgacttta ggaagcagcg acctttcccg gctcggcaca gtacataccc
      601 cgtccttgga atgcatgaga tcccgggcgg aaacatttac ggacagtgaa gaggcctctc
      661 gccttgaagg cgtcaaacct ggtgtaaccc tgccggtttg cctcgccgga gaggtggtgg
      721 gatctatcgc catcgccggc gatcacaggg aggtagcccg ttacggttac ctcgttcaaa
      781 agcaggcgga gctgttcctc agggaacaga tgatccagga atccgcaggg gccagggaca
      841 gggcggtcca ggaactcatc tcctatatcg cttccttcgc ccccggtcat gatgaccccg
      901 gcatcctcgc gccccggatc agggaattgg gctttgagcc cggtatccca agagtctgcc
      961 tggtcttgga gccagggtta gccaagcccg gggcggccgg aaaagggctt gatcccctgg
     1021 aacgggtttc tctctttaag gagatccatg agatcatcct atctgttttc ccggaacctt
     1081 tgaatttcgg tgccagcctg ggaggcgcca ggttcgcgat cttcgccgcc attaaagaag
     1141 tgggaggagg gtccgttctg gatcatgctg tgtcgggatg ccaaagatgc agggagaaaa
     1201 tgaaggggat ggggttgatc ctgacagtgg gggcgggttc gcccgcccat gtcttggagg
     1261 aactccacta ctcctacctt gacgcctgga aagcgctgac aatagggttg aagaagaatg
     1321 atccgggtgg gacccattct attgacgatt accggctgga agaccttttg acgacggtta
     1381 acatgaaaaa ggggcgcctt ttcgccgaca aaaccctttc agggctcagg aaggcccctg
     1441 atggagagga gatgctgcgc accttccatt cctggtgtcg tcacccctgc agccccggac
     1501 tcgtagcgaa ggatctcaat gtccaccgga atacactggg ttacaggata gaaaagataa
     1561 aaaggttgac cggactggat ataagggacc tcggggaggc cttcgaactt tttttagcag
     1621 cgacgctcga tacccttctc gattagcctt gaccgtgggc gacgggtatc gggatgaccg
     1681 ggcgcggact gaagagcgcc cgtccaaacg acgggcgccc ttaatggttc tagaaagaga
     1741 acttattata atccttgggt atttcgaaca gggaccgatc gatggcctct tccttgatgt
     1801 ttttataggt cacgataacc gttccgtctt tcccctcgga gactcccttg atcgggaaat
     1861 tgagtttccg agctacccaa accacggaac tgtccatgtc cctcatgtcg ggatccttgt
     1921 agatcacctc atact
//
LOCUS       DAOSRG010000230         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_54642_length_1929_cov_7.199573, whole genome shotgun sequence.
ACCESSION   DAOSRG010000230 DAOSRG010000000
VERSION     DAOSRG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54642_length_1929_cov_7.199573"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1458)
                     /locus_tag="PLM30_06485"
     CDS             complement(172..1458)
                     /locus_tag="PLM30_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOR54854.1"
                     /translation="MFALCDCNNFYASCERLFRPDLESRPVAVLSNNDGCVIARSAEV
                     KALGVPMGAPYFKEEGRFRHFGVEVFSSNFPLYEDLSRRVSDTLRSMAPDVEIYSIDE
                     AFLRVDALPAAAADPEGFCRRLRERVLQWTGIPVSIGLGATKTLAKAANRIAKRDPSR
                     EGVFAMPGGAGDDPHLEGIDIGDVWGVGLRNAPKLRRFGIGTARGLKYADEEWLKKRF
                     SISEARTSRELNGQVCHPFTERVKPRKTIRVSRSFGNPVMDLHSLREAVSSFAFSAAE
                     TLRKQGSAAGKMHLFLMTNPFGEGPGYAAGATERFDVPASGTVEIVGRATKCLEGIFV
                     GDLAYKKAGVILTDLADAACLQRHLFAPMRDPREGALMGVMDRLNRELGKGTVSTASM
                     GIHGRRSWVMRQERVSPRYTTRWEEIPAVLPPEGAP"
     gene            complement(1543..>1929)
                     /locus_tag="PLM30_06490"
     CDS             complement(1543..>1929)
                     /locus_tag="PLM30_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54855.1"
                     /translation="RAEVMESLGAPDGSEGSVEWYDISGEEGLSRVTLDYLGGEKVQA
                     VHLAFRKGSVDLETLSAIIRDAFPGVSEALGDDRMTVFLGGSADGGGPLYFLALAGDP
                     LGDKGPELISMTEAANRHYQEERPSE"
BASE COUNT          319 a          628 c          636 g          346 t
ORIGIN      
        1 ccacgacgag gacgtcgcct tcttctatgg accaggcggg gatcctcccc gccgatcgca
       61 tcaggaaggt ggacgcccgg ttccggacca gcagtgaact tatgtccagg gggtcctccc
      121 tgaaagggtc ggcgggggag gggaagcccg tggcggaccg ggggtcgccc gtcatggcgc
      181 cccctccggg gggagcacgg cggggatctc ctcccaacgg gtcgtataac ggggcgacac
      241 gcgctcctgg cgcatcaccc aggacctcct gccgtggatg cccatcgagg cggtggagac
      301 ggtccccttg cccagttccc ggttcagccg gtccatcact cccatgagcg ccccctccct
      361 ggggtctctc atgggggcga agaggtgccg ctgcaggcag gccgcgtcgg ccaggtccgt
      421 cagtatcacc ccggccttct tgtaggccag gtctcccacg aagatccctt ccaggcactt
      481 cgtcgcccgg ccgacgatct cgaccgtgcc cgaagccggg acgtcgaacc tctccgtggc
      541 cccggcggcg tagccagggc cctctccgaa ggggtttgtc atcaggaaga ggtgcatctt
      601 cccggcggcc gaaccttgct tcctgagggt ctccgccgcc gagaaggcga agctcgagac
      661 ggcttctctg agggagtgaa ggtccatgac cgggttgccg aagcttctgg atacccttat
      721 ggtcttgcgc ggtttcaccc tctcggtgaa ggggtggcag acctggccgt tgagctccct
      781 ggaggtcctg gcctcgctga tggagaagcg ttttttgagc cactcctcgt cggcgtactt
      841 gagccccctg gcggtgccga tcccgaaacg gcgcagcttc ggggcgttgc ggaggccgac
      901 accccagaca tcgcctatat ctatcccttc gaggtgaggg tcatcccccg cgccgccggg
      961 catggcgaag acgccttccc tggaggggtc cctcttggct atcctgttgg ccgccttggc
     1021 gagggtcttg gtggccccca ggcctatcga tacgggtatc ccggtccatt gaaggaccct
     1081 ctcgcggagg cggcggcaaa acccctcggg gtccgcggcg gcggccggca aggcgtcgac
     1141 gcggagaaag gcctcatcga tggagtagat ctccacgtcg ggcgccatgc tgcggagggt
     1201 gtcggagacc ctcctggaga ggtcctcgta gagggggaag ttcgaggaga agacctccac
     1261 gccgaaatgc ctgaaccggc cctcttcctt gaaatagggt gcccccatgg ggacgcccag
     1321 ggccttcacc tcggcggagc gggctatgac gcagccgtcg ttgttggaga ggaccgccac
     1381 ggggcgggat tcgaggtcgg gtctgaagag cctctcgcag gaggcgtaaa agttgttgca
     1441 gtcgcagaga gcgaacatga tctgacactc ccggaaagcg tgatcccgta aaagattact
     1501 ataagaatac accttccgga atatattgac aagtctgtcg gatcactccg agggccgttc
     1561 ctcctggtag tgccggttgg ccgcttcggt catggagatc agttcaggcc ccttgtcccc
     1621 caggggatcc ccggccaggg cgaggaagta gagcggtccg ccgccgtcgg cgctcccgcc
     1681 gaggaagacc gtcatccggt cgtcgccgag ggcctcggat acgcccggga aagcgtccct
     1741 tatgatggcc gacagggtct ccaggtcgac cgagcccttc ctgaaggcaa ggtggaccgc
     1801 ctgcaccttt tccccgccga ggtaatccag ggtgaccctc gacaaccctt cctcgccgga
     1861 aatgtcgtac cactcgacgg agccttcgga gccgtcgggg gcgcccaggc tttccatcac
     1921 ttccgcccg
//
LOCUS       DAOSRG010000231         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_55954_length_1897_cov_6.176982, whole genome shotgun sequence.
ACCESSION   DAOSRG010000231 DAOSRG010000000
VERSION     DAOSRG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55954_length_1897_cov_6.176982"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLM30_06495"
     CDS             complement(<1..1141)
                     /locus_tag="PLM30_06495"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOR54856.1"
                     /translation="MNPVAFIEDCRDGKGSTPQDIAEFVRLAQKGEIPDYQVAAWLMA
                     VFFRGMTRREVKAFTEALSLSGKVVTFPGDIFPVDKHSTGGVGDKTSLVVVPLAAACG
                     VPVAKLSGRGLGFTGGTVDKLESIPGFSAHLDLGRFVEQVTKVGCAISGHSEDLAPAE
                     ALFYELRDVTGTVPSIPLITSSIVSKKMAGGSRAFVFDVKCGSGAFMKDVASALELSE
                     SLVGLSASLGRKAMALVTAMDQPLGRWVGNAAEVAEAVEVLLRKGPSDVRELSLRLAA
                     SMVHLGGLACSPEEARRQVEEALDSGRAYGKFEEMVHAQGGDLEAFNERAARGGHLAG
                     ATGVIEAKKSGVVARCDARAVGESLRVLGGGRLARTETVDPGAACQ"
     gene            complement(1138..>1897)
                     /locus_tag="PLM30_06500"
     CDS             complement(1138..>1897)
                     /locus_tag="PLM30_06500"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOR54857.1"
                     /translation="AIVLGSGLGSLADSLEDAVTIPIAGIDRWPVSTAPGHAGRLVSG
                     RLGDVPVLMQGRVHFYEGYSPEEVTFPVRVLGEMGVPFYFATNASGGVSHDLSPGDLV
                     VLHDHINLQGFNPLRGRNEDDWGPRFPDMTFTYDRELIAIAEEAASSLGERLKRGVYA
                     AFPGPSFETPAEIRMIRTLGADLVGMSTIPEVIVARHMGMRVCVISCVANYAAGMKEA
                     VMTHEQVLKEMDKAAGRLSRLLKSMVAELGSMVP"
BASE COUNT          327 a          669 c          580 g          321 t
ORIGIN      
        1 cctggcaggc agccccgggg tcgacagtct ccgtccgggc gagccggcca ccccccagta
       61 ccctcaggct ctcccccacc gccctcgcgt cgcaccgggc gaccacgccg ctctttttgg
      121 cctcgatcac cccggtggca ccggcgagat ggcctccgcg ggccgccctt tcgttgaaag
      181 cctcaaggtc gcctccctgg gcgtggacca tttcttcgaa cttgccataa gccctcccgg
      241 agtctagggc ttcctcgacc tgtcgcctgg cctcctccgg ggaacacgcc aggcctccca
      301 ggtggaccat ggaagcggca agccggaggg agagctccct gacgtcgctg ggtcccttcc
      361 ggagcaggac ttccaccgcc tcggccacct cggcggcgtt cccgacccac ctcccgaggg
      421 gctggtccat cgccgtcacc agggccatgg ctttcctgcc cagtgaagcc gaaagcccga
      481 cgaggctttc cgacagttca agggccgagg ccacgtcctt catgaaggcc cccgagccgc
      541 acttgacgtc gaacacgaag gccctggatc ccccggccat ctttttactc acgatgctgc
      601 tggtgatgag cggtatggag ggcaccgtgc cggtaacatc cctcaactcg taaaaaagag
      661 cctccgccgg cgccaggtcc tccgagtgcc cgcttatggc gcagcctacc ttcgtcacct
      721 gttcgacgaa ccgccccagg tcgaggtggg cggaaaaacc gggtatcgat tcgagtttat
      781 cgacggtccc cccggtgaaa ccgaggcccc tgccgctgag cttggccacg gggaccccgc
      841 aggcggcggc caggggcacc acgacgaggc tcgtcttgtc acccacgccg ccggtgctgt
      901 gcttgtccac cgggaagata tccccgggga aggtgactac cttgcccgag agcgacaggg
      961 cctcggtgaa ggcctttact tcccggcggg tcatcccgcg gaagaagacc gccatgagcc
     1021 acgccgccac ctgataatcg gggatctcac ccttctgggc gagccgtacg aactcggcga
     1081 tatcttgggg ggtcgacccc ttgccgtccc ggcagtcctc gatgaaggcg acggggttca
     1141 tggcaccatc gaccccagtt ccgccaccat ggacttcagg aggcgcgaga gcctcccggc
     1201 ggccttgtcc atttctttca ggacctgctc gtgggtcatg acggcctcct tcatcccggc
     1261 ggcgtagttc gccacgcagg agatgacgca gaccctcatg cccatatgcc tggcgacgat
     1321 gacctcgggg atggtggaca tgcccaccag gtcggcaccc agcgtcctga tcatccttat
     1381 ttccgccggg gtctcgaagg aggggccggg gaaggcggcg taaacgcccc gcttgagccg
     1441 ctctcctaac gacgaggcgg cctcctcggc gatggcgatc aactcgcggt cgtaggtgaa
     1501 ggtcatgtcg gggaatctcg gcccccagtc gtcttcattc ctcccacgga gggggttgaa
     1561 accctggagg ttgatatggt catgaaggac caccaggtcg ccgggcgaga ggtcgtgact
     1621 gacgcccccc gaggcgttgg tggcgaaata aaagggcaca cccatctctc ccaggaccct
     1681 cacgggaaag gtgacctcct cgggtgaata gccttcgtag aagtggaccc tcccctgcat
     1741 cagcacgggg acatcgccca ggcgacccga caccagcctg ccggcatggc ccggggccgt
     1801 tgacacgggc caccggtcta tccccgcaat aggtatcgtc acggcgtcct ccaacgaatc
     1861 ggccagggat cccagtccgg agcccagcac gatggcg
//
LOCUS       DAOSRG010000232         1896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_56000_length_1896_cov_6.394894, whole genome shotgun sequence.
ACCESSION   DAOSRG010000232 DAOSRG010000000
VERSION     DAOSRG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56000_length_1896_cov_6.394894"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1251
                     /locus_tag="PLM30_06505"
     CDS             292..1251
                     /locus_tag="PLM30_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR54858.1"
                     /translation="MKVLDLEAAPDVTLSLIDLPLAMEGYHHFFGVWLVRDEGRGRNV
                     IVDPGPASTAPLLLETLGGLGVDRLDLVLLTHIHLDHSGGLAELLEAFPAAMVNVHPR
                     GKPHLADPARLWESSLRVIPEMAPAYGEPLPVDEGRFIPEGAEIPGILAVDTPGHAPH
                     HRSFFYETPAGPVLFAGEAAGTFTRLDTVVPGADEGKYVLRPASPPRFYIDQALQSIE
                     TLKAVNAILLCYAHFGYSKEVSRMLDEAARQIQLWRVLFAEFLEGKGRPGADQVDMDG
                     LLSFLLSRDPWLEAFPMLPSDVRSREMNFMLSSASGFLETVTA"
     gene            complement(1252..>1896)
                     /locus_tag="PLM30_06510"
     CDS             complement(1252..>1896)
                     /locus_tag="PLM30_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR54859.1"
                     /translation="LDLAGHGHSGLGRSRYTMESFGEDVAAVVKAAGAERAILIGHSM
                     GGQVIACAARILPGRVSGLIGVDTLENVGYVMTPEEREAMLAPFREDFPAETARFAGE
                     MLSPAVERSLREWIVSDMSSAPSWVAVSAMEEMTSQYVTGEAARVFEEVRLPVVSVNA
                     DKWPVDFKANREHMLSYEAIVIADADHFLMMTRSEEFNDALERAIGMILWEAGK"
BASE COUNT          341 a          634 c          551 g          370 t
ORIGIN      
        1 agagctcccc ccggaggatc aacttcttga tctcttcata aaccaggtcg gtcttagatc
       61 tcttcctcaa gctttgcatc ccctcaccgg tatacaactg ttatgcatcc gaaatgtaac
      121 cgatatgcga aagttagacc tcttttaccc gattgtcaag ccttttacga acggggtgtg
      181 tcgacgggaa cggagcatcg ttgccgttgt cggatcgtgt cagccggggg ataatgtccc
      241 agtcgcgggc ggaaggtttt aaacaaggat cggtttttga ggtgaacagt catgaaagta
      301 ttggatctgg aagcggcacc cgatgtcact ctgagcctta tcgacctccc cctcgccatg
      361 gagggctatc accacttctt cggggtctgg ctggtcaggg acgagggtag ggggcggaat
      421 gtcatcgtcg acccgggacc ggcctcgacg gctccactcc ttctcgaaac cctgggtggc
      481 ctgggcgtcg acaggctgga cctggtcctc ctgacccata tccacctgga ccactccggc
      541 ggcttggccg aactcctcga ggccttccct gccgccatgg tgaacgtcca ccccaggggg
      601 aaaccccacc tggcggaccc cgcgaggctc tgggagagca gcctgcgggt catacccgaa
      661 atggcaccgg cctacggtga acccttgccg gtggatgagg gccgcttcat ccccgaaggg
      721 gccgagatcc cggggatcct cgccgtcgac acccctgggc acgcgcccca ccaccgttcc
      781 tttttctacg agaccccggc gggcccggtc ctcttcgccg gcgaggccgc cggcaccttc
      841 acccgcctcg acacggtcgt cccgggggcc gatgaaggca agtacgtgct acgaccggcc
      901 tcgccgcccc gtttctatat agaccaggcg ctgcagtcca tagagacgct gaaggcggtg
      961 aacgccatcc tgctctgcta tgcccacttc ggctattcca aagaagtgag taggatgctg
     1021 gacgaggctg cccggcagat ccaattatgg agagtactct tcgcggagtt ccttgagggg
     1081 aagggacgcc ccggggccga ccaagtagat atggacggac tgttgtcttt cctgttgagc
     1141 cgtgacccct ggctcgaggc gttcccgatg ctcccctccg acgtgcggtc ccgggagatg
     1201 aacttcatgc tctccagcgc gtccggcttc ctggagaccg tcaccgcctg actacttgcc
     1261 cgcctcccaa aggatcatcc cgatggccct ttccagggcg tcattgaact cctcggaccg
     1321 ggtcatcatc aggaagtggt ccgcgtcggc gatgacgatg gcttcgtagg agagcatgtg
     1381 ttcccggttg gccttgaaat ccacgggcca cttgtcggca tttacgctga ccacggggag
     1441 gcggacctcc tcgaagacgc gggccgcctc accggtgacg tactgggagg tcatctcttc
     1501 catggcgctc accgccaccc aggagggcgc cgaggacatg tccgatacga tccactccct
     1561 cagcgacctc tccacggccg gtgacagcat ctccccggcg aaacgggccg tctcggccgg
     1621 gaaatcttcc ctgaaggggg ccagcattgc ttcccgctcc tcgggcgtca taacgtaccc
     1681 gacattctcc agggtatcca cgccgatgag ccccgacacc ctcccgggga ggatcctcgc
     1741 ggcgcaggct atgacctgcc ctcccatgga atggccaatg aggatggccc tttccgcccc
     1801 cgccgccttt accacggctg ccacgtcctc accgaaggac tccatggtgt acctggaacg
     1861 ccccaggccc gaatgaccgt gacccgccag gtccag
//
LOCUS       DAOSRG010000233         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_56304_length_1889_cov_9.214286, whole genome shotgun sequence.
ACCESSION   DAOSRG010000233 DAOSRG010000000
VERSION     DAOSRG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56304_length_1889_cov_9.214286"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLM30_06515"
     CDS             complement(<1..1142)
                     /locus_tag="PLM30_06515"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOR54860.1"
                     /translation="MQHTDDRSSFSERLARPVSRSLLRDVTEDELRAMAAHEAVPNDQ
                     GASLYVTRIRSRSAAFTDIVYEPDEPLVALLEQVWGYLRWQQMIRVTARIGSPDRRPM
                     LANFYVTRRYARLAQMFAMNFSTELDQDYSDIVTVAIPEWHQRKIIVLPRQRVTYILG
                     SDYYGEAKMATLRMVMHLGRETMDALGLHAGSKIIRVNTPRGLEEKGVLIFGLSGTGK
                     TTITTADHDLEAPEGVEVLQDDINILLSNGSALGSEANFYIKTDNVTKQPVLLWAARD
                     RKALIENCWVDDDDHINFDDHALTTNGRAVVPREAIPNTSDRIDLDKVDCLLFNMRRY
                     DTPAIGRLVSPEQAAAYFMLGESTITSADDPSRVGQAKRVVGFDPFV"
     gene            complement(1161..1787)
                     /locus_tag="PLM30_06520"
     CDS             complement(1161..1787)
                     /locus_tag="PLM30_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOR54861.1"
                     /translation="MTGGDEFFEPATFAEVETRHKRSLFTGRVFRTGSEEEAREHVRE
                     ISKRYADATHHCWAYRVGFPQTTEYYSDAGEPSGTAGRPILGAIHRAGVVNTVVVITR
                     YYGGTKLGVRGLIEAYGECASLALAAAGRRGRVRSREASLECPYEHARLILKQLADLG
                     LPEGAVEAEWGSAVTFSASVPLSLADDAASLFENYKERGFITRWGWKK"
BASE COUNT          314 a          599 c          593 g          383 t
ORIGIN      
        1 acgaagggat cgaagcccac cacccttttg gcctggccga ccctggaggg gtcgtccgcg
       61 ctggtgatgg tcgattcccc cagcatgaag taggccgcgg cctgctccgg cgagacgagg
      121 cgtccgatgg ccggggtgtc gtacctcctc atgttgaaaa gcagacagtc gaccttgtcc
      181 aggtctatcc ggtccgatgt gttgggtatc gcctcccggg ggacgacggc cctgccgttg
      241 gtggtcaggg cgtggtcatc aaagttgata tggtcgtcgt catccaccca gcagttctcg
      301 atgagggcct tcctgtcccg ggcggcccag aggaggaccg gttgcttcgt cacattgtcg
      361 gtctttatgt agaagttggc ctccgaacca agggcgctgc cgttggagag gaggatattt
      421 atatcgtcct ggagcacctc cacaccctca ggcgcttcca ggtcatggtc ggccgttgtt
      481 atggtggtct tgccggtgcc ggaaagcccg aagatgagca ctcccttctc ctccaacccc
      541 ctgggagtgt tcaccctgat gatcttgctg ccggcgtgaa ggccgagggc gtccatcgtc
      601 tcgcgcccca ggtgcatgac catcctcagg gtggccatct tggcctctcc gtagtagtcg
      661 ctacccagga tgtaggtgac tcgctgcctg gggaggacga tgatcttacg ctggtgccac
      721 tcgggtatgg ccacggtgac aatgtcggag tagtcctggt caagttccgt ggagaagttc
      781 atggcgaaca tctgggccag cctggcgtac ctgcgggtca cgtagaagtt cgccagcatg
      841 ggccggcggt ccggggagcc tatcctggcc gttacgcgga tcatctgctg ccagcgaagg
      901 taaccccaca cctgctccag aagagccacc agtggttcgt cgggctcgta gacgatatcg
      961 gtgaaggcag cgctcctgga gcggatcctg gtcacgtaga gggaggctcc ctggtcgttg
     1021 ggcacggcct cgtgggccgc catggccctg agctcatcct cggtgacgtc cctgaggagg
     1081 ctacggctca cggggcgggc cagcctctcg ctgaaagatg aacggtcatc ggtgtgctgc
     1141 actgacgatc gcctccagtc ctattttttc catccccaac gggtgatgaa gcctctttct
     1201 ttgtaatttt cgaagaggga agcggcatcg tcggcgaggg acaagggcac cgacgcggag
     1261 aaggtcactg cggaacccca ctccgcctcc acggcaccct cgggcaggcc gaggtccgcc
     1321 agctgcttca ggataagccg ggcgtgctcg taggggcact ccagggaggc ctccctggac
     1381 cttacgcgcc cccttcgccc cgccgccgcc agggcaagcg atgcgcactc gccgtaggcc
     1441 tcgatgagcc ctctcacgcc caacttcgtc cccccgtagt accgggtgat caccaccacc
     1501 gtgtttacaa cgccggcccg gtggatggcc cccaggatgg gcctgcccgc cgtgcccgaa
     1561 ggctcgccgg catcggagta gtactccgtc gtctgaggga aacccaccct gtaggcccaa
     1621 cagtgatgcg tagcgtcagc gtatcgcttg gatatctctc tgacgtgttc ccgggcttcc
     1681 tcttcgctgc ccgtgcgaaa gaccctgccg gtgaaaaggg agcgcttatg cctcgtttcg
     1741 acctcggcga aggtagccgg ctcgaagaac tcatcgccgc cggtcatggg cctcccaaac
     1801 cttcttcagg ctctcccagg tctcctggag cgatgccggc aggaccttcg tgccggctat
     1861 gacgggcatg aagttggtgt ccccgttcc
//
LOCUS       DAOSRG010000234         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_56349_length_1888_cov_10.345336, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000234 DAOSRG010000000
VERSION     DAOSRG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56349_length_1888_cov_10.345336"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLM30_06525"
     CDS             complement(<1..272)
                     /locus_tag="PLM30_06525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HOR54862.1"
                     /translation="MNGFPLYPVGFVRSPFKKPMPPEEFRGTLSRIELRPEFEEALEG
                     LQEGELLLVLFRFHLCRGFPMKVHPRGDPANPLRGVFSTCSPYRPN"
     gene            complement(269..1213)
                     /gene="trmB"
                     /locus_tag="PLM30_06530"
     CDS             complement(269..1213)
                     /gene="trmB"
                     /locus_tag="PLM30_06530"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOR54863.1"
                     /translation="MPVLMPLGEILVKSDPHNLVSMISGEHAPYKAVEIGFGNGEFLL
                     QRASERPDGLFYGIEVSRNSIMKAMKRAKRSFSRNVRFFYGDARFILNECFPEAWLDA
                     VYMNFPCPWPKNRHAKRRVTTRYFVNSLAGVLKVGGSFELVTDDRQYAEEAAAMIPSH
                     PAIVIDLFEPDPFRSLRTKYEKKWLEAGKRIFLLRFIKEKPFGKKDPKKGVIGLHKSL
                     GRPCPALSELALLVGKRAGQREARWVFRDCFVNPSGVILVETLTSDEGFEQTFFFRIV
                     PRSDGCLIKTDPATRPFTTPSVAGAFKDVTSLLEDGAQ"
     gene            1513..>1888
                     /locus_tag="PLM30_06535"
     CDS             1513..>1888
                     /locus_tag="PLM30_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR54864.1"
                     /translation="MSQVIEVRWHGRGGQGAKTGSAILAEVLFEAGKHTQSFPEYGAE
                     RQGAPMKAFNRVGDEPIRDRSGVLTPDVVVLIDPTLIDAVKPTVGGKDSTVYLINSPD
                     SPANLKKKLGLKDSVKVMTVDAT"
BASE COUNT          438 a          516 c          513 g          421 t
ORIGIN      
        1 tggttaggcc tgtaagggct gcaggtcgag aacacccccc tgaggggatt ggcgggatcc
       61 cccctcgggt ggactttcat ggggaagccc cgacaaaggt ggaacctgaa aagtaccagt
      121 agcagttcac cctcttgcag cccttccaag gcttcttcga attcgggtct cagttcgatc
      181 cttgaaaggg tcccgcggaa ttcctccggc ggcataggtt tcttgaaggg cgagcgcacg
      241 aaacccacgg ggtaaagggg aaaaccattc attgtgcccc atcctccagc aaggaagtca
      301 catccttgaa cgctcccgcc accgaggggg tcgtgaaagg ccgcgtcgcg ggatccgttt
      361 tgatcagaca cccgtcactt ctcgggacga tccgaaaaaa gaatgtctgt tcgaatccct
      421 catcggaggt aagggtctcc accaggatca ctcccgaagg gttgacaaag cagtccctga
      481 agacccagcg agcttccctt tgtccggccc ttttccccac caggagggcc aattccgata
      541 atgccgggca gggccttccc aaagacttgt gcaacccgat cactcccttt ttcgggtctt
      601 tcttcccgaa gggtttttcc tttatgaagc gcaacagaaa gatccttttg cccgcttcca
      661 gccacttctt ttcgtacttg gtgcgcagac ttctgaaagg gtccggttca aaaagatcga
      721 tcacgatggc ggggtgggaa ggtatcatcg ccgccgcttc ttcggcgtat tgccggtcgt
      781 cggtcaccag ttcgaaggat ccccctacct tgagaacccc cgccaggctg ttaacgaaat
      841 accttgtggt cactctcctc ttggcgtgcc tgttcttagg ccaggggcag ggaaagttca
      901 tatagacagc gtccaaccat gcttcgggga agcattcgtt caggatgaac cgggcatcgc
      961 catagaaaaa acggacgttg cgggagaagg atcgtttcgc ccgtttcatc gccttcatta
     1021 tggaattacg ggaaacctcg atgccgtaaa aaagtccgtc aggcctttcg gaagcccttt
     1081 gaagcaggaa ctcgccgtta ccgaacccga tttcaaccgc cttataaggg gcatgctccc
     1141 cggagatcat tgagaccagg ttgtggggat cgctttttac cagtatctct cccaggggca
     1201 tcaaaaccgg cacatccttt cgcggataaa ggcggtggca gtttgaaaat attcttcagg
     1261 cttgacacaa ggttccgcct acatacaata tacatgcaat agacatataa ttctgaagag
     1321 cccattctac ctgtttgtcc ctggggaatt ataagccatg aggctccagt tcattcccgg
     1381 cggcttcgat gacccaggga gcgggatgcg gtagggtgag ggtttctttc ctttccatgt
     1441 tattatttta ggcgtcttca gaattagccg caattaaccg ttcaacgtat ctttcaagaa
     1501 ggaggggtac taatgtcaca agtcatcgaa gtccgatggc acggaagagg gggacaggga
     1561 gccaagacgg ggtccgcgat cctggctgag gtgcttttcg aggccggcaa gcacacccag
     1621 tccttccccg agtacggagc agaacgtcag ggggcgccaa tgaaggcctt caatagggtg
     1681 ggagacgaac cgatcaggga cagatccggg gtcctcacgc cggatgtagt ggtcctcatc
     1741 gatccaacgc tgatcgatgc ggtcaagccg acggtgggcg gcaaagattc cactgtctac
     1801 ctgatcaact cgccggattc tccagcaaac ctaaagaaaa aactggggct gaaggattct
     1861 gtaaaggtca tgaccgtcga cgccacca
//
LOCUS       DAOSRG010000235         1868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_57211_length_1868_cov_7.965251, whole genome shotgun sequence.
ACCESSION   DAOSRG010000235 DAOSRG010000000
VERSION     DAOSRG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57211_length_1868_cov_7.965251"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLM30_06540"
     CDS             complement(<1..831)
                     /locus_tag="PLM30_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOR54865.1"
                     /translation="MFDSLRERLESVFKGLRGKGKLTEEDISAAMRDVRRALLEGDVN
                     FRVVKDLVERMRERAVGRQVLESITPAQQVIAIVYEEMAALMGSGRRSLTFSSKPPTR
                     YILVGLQGSGKTTTCVKIAKKVSDRHKPLVVACDLRRPAAVEQLRILADQAGVGFFGP
                     SDGEGDVIRLAGKAMEYAAQRLYDVLLFDTAGRLDIDELLMGELASLGEALSPTETLL
                     VVDAMTGQEAVSVAIAFNERVPLSGIVLTKLDGDSRGGAALSIGAATGIPVKLAGVGE
                     R"
     gene            complement(840..1196)
                     /locus_tag="PLM30_06545"
     CDS             complement(840..1196)
                     /locus_tag="PLM30_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOR54866.1"
                     /translation="MDDLIEQRVTFSRLFDFYEPALTEKQKEAFRLHVMEDWSLSEVA
                     ERLEVTRQGAHDLVQRARGRLLELEGLLSFSAREAAWESRMEKLREWRRRHLSRIPAE
                     AAAELEALLATEEGEV"
     gene            complement(1385..1669)
                     /locus_tag="PLM30_06550"
     CDS             complement(1385..1669)
                     /locus_tag="PLM30_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54867.1"
                     /translation="MCRVITPDQGGGHGHTDDPLSIAELLRETLSQELDALTELAARH
                     HMIEDEEVRHVLGHLIGSRRKDLAELWGLLQRIEDKAFGDHPHGHGHEHK"
BASE COUNT          354 a          617 c          519 g          378 t
ORIGIN      
        1 cctctccccg accccggcca gcttgacggg tatccccgtc gcggccccta tgctcagcgc
       61 agctccgccc ctggaatcac cgtcgagttt cgtcaggacg atcccgctca gggggacccg
      121 ctcgttgaag gcgatcgcca cgctgacggc ctcctgtccg gtcatggcgt ccaccacgag
      181 aagggtctcc gtaggtgaga gggcttcccc gagggaggcc agttcaccca tcagcagctc
      241 gtctatatca agccggccgg cggtgtcgaa aaggagtacg tcatagagcc tctgggccgc
      301 gtattccatc gctttcccgg cgagccggat cacgtcgccc tctccatcgg aaggcccgaa
      361 gaaccccacc ccggcctggt cggccagtat cctgagctgt tccaccgcgg cgggcctcct
      421 caagtcacaa gcgacgacca ggggcttgtg acggtcggag accttcttgg ctatcttgac
      481 gcaagtcgtt gtcttgcccg atccctggag gccgacgagg atgtacctgg tcggtggctt
      541 cgaggaaaag gtgagcgacc gacgccccga tcccatcaac gccgccattt cctcgtagac
      601 gatagcgatc acttgctgcg cgggggtaat cgactcgagg acttgcctcc cgacagcccg
      661 ttctcgcatc ctctcgacca ggtccttgac gacccggaag ttgacatccc cttccaggag
      721 ggctcttctg acgtccctca tggcggcgga tatatcctct tccgtcagtt tccccttgcc
      781 cctcaggcct ttgaataccg attcaaggcg ctccctgagt gaatcgaaca tggacggggt
      841 cagacctccc cttcttccgt ggcaaggagg gcctccaatt ccgccgcggc ctcggcgggg
      901 atccgggaca gatgacgccg gcgccactca cggagttttt ccatgcggct ttcccaggcg
      961 gcctcccttg ccgagaaact gagcaggccc tccagctcca ggaggcggcc cctcgccctc
     1021 tgcaccaggt catgggcgcc ctggcgggtg acctcgagcc tttcggcgac ctccgagagc
     1081 gaccagtcct ccatgacatg aagccggaag gcctcttttt gcttttccgt cagggccggt
     1141 tcgtaaaaat cgaaaaggcg tgaaaacgtc accctctgtt cgatcaggtc gtccaacggg
     1201 gcacctccaa gcgcacacgg acaatgatta taaggagtct ccccaggcct gtcaagcgtc
     1261 gccccttgac agctttcgac ctcgagttct ccccaaaaaa gagaatgaaa aagccggggt
     1321 tcgatccccg gcatgaaagt cctaaccctg ttcctaaaag aactggtctt cagcgaaggg
     1381 ccgcttattt atgctcgtgt ccgtgcccgt ggggatgatc gccgaaggcc ttatcctcta
     1441 tgcgctgaag aaggccccac aactccgcca ggtccttcct cctggaccct atcaggtgtc
     1501 ccaggacgtg gcggacctct tcgtcctcga tcatatgatg ccgtgccgcg agttccgtca
     1561 aggcgtcgag ctcctgggag agcgtctccc tcaggagttc cgcgatcgac agggggtcat
     1621 cggtgtggcc gtgtcccccg ccctggtccg gtgtgatcac tctgcacatg gtcatacctc
     1681 ctttgtcttc ggcataagag aataacacat gccggggatt ctgtcacacg aataacaaaa
     1741 aatctcttcc gaaaaagtcc gaccctcccg cggggcttcc gccgttctga taaaatgaag
     1801 gccgtccgtt gaaggtcccg cgggatcact aatgacgaaa ggggtaatgc tgatgaactc
     1861 gacccagg
//
LOCUS       DAOSRG010000236         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_57315_length_1866_cov_8.524020, whole genome shotgun sequence.
ACCESSION   DAOSRG010000236 DAOSRG010000000
VERSION     DAOSRG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57315_length_1866_cov_8.524020"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLM30_06555"
     CDS             complement(<1..809)
                     /locus_tag="PLM30_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR54868.1"
                     /translation="MKLSQDVTWRLLQGFGWIVNRAPHGLAVRLGEWVGVLVWFFSRA
                     RVDRAEARCVRVLQVGVTTARAIVKESYRNLGRGLAEALRLPSLCPGLMDYIDIHGEE
                     NLKEALTKGKGVVALTAHFGNWEFAAAALALKGYPMNAIGAEQRDPRITDLVVSLRSS
                     CGVKTMSRGFDLKSAVRCLKKGEILGVLLDQDFGDDGIVVPFMGLPASTPFGPVKMAD
                     RIGSAVVPIFIVRRPDGVHHDLYIEPALGEAGGAPFGEKVEASLELCNDTLG"
     gene            915..1712
                     /locus_tag="PLM30_06560"
     CDS             915..1712
                     /locus_tag="PLM30_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR54869.1"
                     /translation="MFRKILFPIDLSDLSRKGLAWTARNVADPSSEMVLVHVLEPIVG
                     MDTGSMVHQAERSIGIIEAELTSIGIRNKSFVVVGDPYEILPEIAHSEGCSLCALFAD
                     REDVVVPSLRLMALPHLVFKASGGVMPPPDPFRRITVAADLSPTRTDVVFRELKKLLA
                     GRVVPMTILHSLFMEDTLTSTEMFQAATAALEEVRASVEKWNPGTGAEVLGGHPEVEI
                     LRRVEKIKPGMLVVGLSVHGHFWELIVGSTGEAIIERSPCPVLVIPS"
BASE COUNT          333 a          624 c          574 g          335 t
ORIGIN      
        1 cccagggtat cgttgcacag ttccagggag gcctccacct tctcgccgaa gggggcccct
       61 ccggcctcgc ccagggcggg ctcgatgtaa aggtcgtggt gcacgccatc gggtcggcgg
      121 acgatgaaaa tcggtaccac ggcggaccct atcctgtcgg ccatcttgac cggtccgaag
      181 ggggtgctcg ccggcaagcc catgaaaggg accacgatgc catcgtcgcc gaagtcctgg
      241 tcgaggagca cacccaggat ctcgcctttc ttcaggcagc ggacggcgct cttgaggtcg
      301 aacccccggc tcatggtctt cacaccgcag gatgagcgca gggatacgac caggtccgtt
      361 atacgggggt cccgctgctc ggcgccgatg gcgttcatgg ggtaaccctt caaggcgagg
      421 gcagcggcgg cgaactccca gttcccgaag tgggccgtca gggctacgac ccccttccct
      481 ttcgtcaggg cttccttgag gttctcctcg ccgtggatat cgatatagtc catgagcccc
      541 gggcagagtg aagggagccg gagcgcttcg gccagccctc tccccaggtt gcggtaggac
      601 tctttcacga tggccctggc cgtggtcaca ccgacctgga ggaccctgac acacctggcc
      661 tcggcacgat cgaccctggc ccggctgaag aaccacacca gcacccccac ccactcgccg
      721 agcctcaccg cgaggccgtg gggcgccctg ttaactatcc acccgaagcc ctggagcagg
      781 cgccaggtga catcctggga cagtttcaaa ggcggacctc ctcacaagag gatgaacgat
      841 cggcatcctt gacggtatac catcatctgt taaactcatt ttagtctagc acaaaattaa
      901 agggggcatc ggccatgttc agaaagatcc tcttcccgat cgatctttcc gacctctcac
      961 gaaaggggct ggcctggacg gcccggaacg tggccgaccc gtcctcggaa atggtcctgg
     1021 tccacgtcct cgagcccata gtcggcatgg acacgggctc gatggtccac caggcggaaa
     1081 ggtcgatagg catcatcgag gcggaactca cttccatcgg catcaggaac aagtccttcg
     1141 tcgtggttgg cgacccctac gagatactgc cggagatagc ccattccgag gggtgctccc
     1201 tgtgcgcgct tttcgccgac cgggaggacg tggtggtgcc ctccctgcgg ctcatggccc
     1261 tgccccacct cgtcttcaag gcgtcgggcg gcgtcatgcc cccacccgac cctttccgca
     1321 ggataacggt ggcggcggac ctctcgccca ccaggaccga cgtcgtcttc agggaactga
     1381 aaaagctcct ggccggcagg gtcgtcccca tgaccatcct gcactccctg ttcatggagg
     1441 acaccctcac ctcgaccgag atgttccagg ccgctacggc ggcgctggag gaggtgcggg
     1501 cgtcggtgga aaagtggaac cccgggacgg gggccgaggt gctgggcggt caccccgagg
     1561 tggagatcct ccgcagggtc gagaagatca agcccggcat gctcgtggtg gggctctcgg
     1621 tccacgggca cttctgggaa ctcatcgtcg ggagcaccgg cgaagccatc atagagcggt
     1681 cgccctgccc cgtcctggtt atcccctcct gaagccctcc cttcaggggc gctccctgga
     1741 ggcggcctcc atttcctgca gtatgcggag gatggccgag tcggcggcca tgagcccttc
     1801 cctgctgaaa tcgcccacgt tcttcgccgt cacggcgaag tcggaggata tcatcccctg
     1861 gtcccc
//
LOCUS       DAOSRG010000237         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_57422_length_1864_cov_6.334439, whole genome shotgun sequence.
ACCESSION   DAOSRG010000237 DAOSRG010000000
VERSION     DAOSRG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57422_length_1864_cov_6.334439"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>1864
                     /locus_tag="PLM30_06565"
     CDS             117..>1864
                     /locus_tag="PLM30_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HOR54870.1"
                     /translation="MKKRYGPLDRQEPRSPSFSRNIAMAVCGLAALLLLCGFKPTTGF
                     AASILSFRAGLQPGGVRLVIDLDEPAEYTVTTDASTILLDIDAMSAAPASGRFLRNTA
                     AEAFTSKPSEGRTFLEVRLKSPSVTVRHFTLPMPDRVVLDLFPSPRSGGSGDARPQVQ
                     ALHGLIPSQPIDGQGKGYRDLRLLPEVLLLEKGLAVKTFPFHLPATKSIVGGGSFRPV
                     LRFHGPLEQDRARVTFILNDVPLEEIVLVGAAAGEQVSDIDLPATLLRPGKNAMTLAA
                     MTEPKNGVKDGEGSLAILDGSFLRLDYLDASDFSLGDMAAFLEGGPPFPDRGLVVVIP
                     DEPGPEEVQAAMELLLQWNGGSPSGTAPGRVLSVGSVTPGIADSSHMIYLGRGGSFPR
                     SVLKAFHWEPPSQETSFLSCFVNNYGKLRLLITSETAEGILGGATPLLDRGLRGSLNA
                     RRMYVSPIKALPKPGGGSRKGQVTFKDLTGGDLVLSGAGKSSRRIEIMVPPGDLAGSR
                     TEVSLSFRSSPLIDGERSTLTLFLGGAPAASVNLGKSRIGKDQLATRIPKVLHGASFL
                     AVEIEAYLVPFPGGEEV"
BASE COUNT          334 a          561 c          585 g          384 t
ORIGIN      
        1 atatcgtgga agaactgcct ctttccagcg tagggaaggt gctcaggagg aaacttaagg
       61 aggcggaggg agaggacctc tagtggagat ggccggccag gctagaggat aataggatga
      121 aaaaaaggta tggccccttg gaccgccagg aaccgcggtc cccgtctttc tcgagaaata
      181 ttgcgatggc ggtctgcggg ctcgctgctt tgctactcct ttgcggtttt aagccaacta
      241 ccgggttcgc cgcctccatc ctttccttca gggccggcct ccagcccggg ggagtgaggc
      301 tggtcataga cctggacgaa cccgcagaat acacagtaac gactgacgct tcgacgatcc
      361 tgctggatat agatgcgatg tccgcagccc cggcttcggg gcgcttcctc cgtaacaccg
      421 ccgccgaggc tttcacatcg aaaccttccg aggggcgcac tttcctggag gttcgcctca
      481 agtcgccctc tgtcacggtc aggcacttta ccctccccat gccggatagg gttgtactgg
      541 acctattccc ctcccctcga tcggggggat cgggtgacgc aaggccacag gtccaggctc
      601 tccacgggtt gataccttcc cagccgatcg atggacaagg gaaggggtat agggaccttc
      661 gcctcttgcc ggaggtactt ctgcttgaga agggccttgc tgttaaaacc tttccctttc
      721 atctgccggc cacgaaaagc atcgtgggtg gcggttcttt caggcctgtg cttcgtttcc
      781 atggacccct ggagcaggac cgggccaggg tgacttttat cctgaacgac gttcccctcg
      841 aggagatcgt tcttgtcggc gcggccgccg gtgagcaggt gtccgatatc gacctgcccg
      901 cgacgctttt gcgccccggg aagaacgcga tgaccctggc cgcgatgacc gagccaaaga
      961 acggggtcaa ggatggcgaa ggttccctcg ctattctcga cggaagcttc ttgcgcctgg
     1021 actacctgga cgcctctgac ttcagcctgg gagacatggc ggccttcctc gagggtggcc
     1081 ctcccttccc ggaccggggc ctggttgtgg tcatcccgga tgagccgggg ccggaagagg
     1141 tccaggcggc gatggaactc ttattgcaat ggaatggtgg gtctcctagc ggcacggcac
     1201 ccggcagggt gctgtcagtg ggctcggtca cgccagggat agcggactca tcccacatga
     1261 tctacctcgg gcggggcggc tcttttcccc gctcggtcct aaaggctttc cactgggaac
     1321 ctccttcgca ggagaccagt ttcctgtcat gcttcgtgaa caactacgga aagctcagac
     1381 tcctgatcac ttcggagacg gccgagggga tccttggcgg cgccaccccc ctgctggacc
     1441 gcgggcttcg tggtagtttg aacgccagga ggatgtacgt atccccaatt aaggcccttc
     1501 cgaaaccagg cggtgggtcg cggaagggcc aggtcacttt caaggatctg accggcgggg
     1561 acctggtcct cagcggtgcg gggaagtcat cccgccggat cgagatcatg gttcctcccg
     1621 gagacctggc cggaagccgg acggaggttt cactttcctt ccgctcctct cccctcatcg
     1681 acggtgagcg gtccaccctg accctctttc tcggcggggc accggccgcc tcggtcaacc
     1741 tgggcaagtc caggataggg aaggatcagt tggcgacgag gattcccaag gtgctccacg
     1801 gggctagctt cctggccgtg gagatagagg cctaccttgt gccgttcccg ggaggggagg
     1861 aagt
//
LOCUS       DAOSRG010000238         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_57708_length_1858_cov_7.488075, whole genome shotgun sequence.
ACCESSION   DAOSRG010000238 DAOSRG010000000
VERSION     DAOSRG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57708_length_1858_cov_7.488075"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLM30_06570"
     CDS             <1..344
                     /locus_tag="PLM30_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOR54871.1"
                     /translation="TPQDLSVRIVEKMMHMTKLMDVPLLGIVENMSFALCPHCGERWE
                     LFGPSHLEEVRSRWDIPALASLPVDKEITTLADSGRLEEYRSGDEPEEMAARVLSAAA
                     SAVTTPSGGPR"
     gene            complement(316..1176)
                     /locus_tag="PLM30_06575"
     CDS             complement(316..1176)
                     /locus_tag="PLM30_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54872.1"
                     /translation="MKIREITVVSGKGGTGKTSITAALAASAPGGLALCDADVDAPDL
                     EILLHPEPREEHAFIGMDTAGVDSQRCIGCGKCRDVCRFDAIRMEGGKARVDKTFCEG
                     CAACTLVCPVGCIAMEETRQGTWFVGETGFGPMVHARLNPGGENSGMLVQLVRKEARR
                     IAESRGLGTILTDGPPGIACPAISAVTGADTALVITEPTISGEHDLLRVAELCRRFGT
                     KAAVVLNKADLSTQGAERIKEACRREGLPLLASIPFIPGVVHAVSQAMIPLAELEEFL
                     GPTWDRLKGL"
     gene            complement(1173..>1858)
                     /locus_tag="PLM30_06580"
     CDS             complement(1173..>1858)
                     /locus_tag="PLM30_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR54873.1"
                     /translation="LVEIPWPVIDNVLCNRCGECVRSCRFGALLMFGGGAPVPNDLCH
                     GCGVCSMVCPQKAITEVGRPVGEVRLCRRGDLRLYEGRLHVGMPNPVPVIDATLKAAL
                     AEGSYLVIDSPPGTSCPMVSAVREADVVVLVTEPTPFGYADLDLALQVIHELGKPAMA
                     VVNRCDLGDADIEGLTERYGVPVAARLPFSRAVAESYAAGNPPAATDALWRDAMVTLW
                     ERAKEMAGR"
BASE COUNT          294 a          659 c          597 g          308 t
ORIGIN      
        1 ccacgcccca ggacctctcg gtccgcatcg tcgaaaaaat gatgcacatg accaaactca
       61 tggacgtgcc cctgctgggc atcgtggaga acatgagctt tgccctctgc ccccactgtg
      121 gcgagcgctg ggaacttttt ggcccgagtc acctggagga ggtccgctca cggtgggaca
      181 tacccgctct ggccagcctc cccgtggaca aggagatcac gaccctggcc gattcgggca
      241 ggctcgagga gtaccgttcc ggggatgagc ccgaggagat ggccgccagg gtgctctccg
      301 ctgcggcctc ggcggtcaca accccttcag gcggtcccag gtaggtccca ggaactcctc
      361 cagttccgcc agggggatca tcgcctggga gacggcgtgg accacgccgg ggataaaagg
      421 aatggaagcg agaagaggca ggccctccct acggcatgcc tcttttattc tttccgcgcc
      481 ctgggtgctc aggtcggcct tgttcagcac caccgccgcc ttggtcccaa agcgccggca
      541 gagttcggcg acccggagca ggtcgtgttc gcccgagatc gtcggctcgg tgatgacgag
      601 ggccgtgtcg gccccggtga cggccgaaat ggcggggcag gctatcccgg gggggccgtc
      661 ggtgaggatg gtcccaagcc ccctgctttc ggcgatccgc cgggcctcct tccggaccag
      721 ctgtaccagc atccccgagt tctccccgcc ggggttgagc ctggcatgca ccatgggacc
      781 gaagcccgtc tcgccgacga accaggtccc ctgcctcgtc tcctccatgg cgatgcagcc
      841 cacggggcag accagggtgc aggcggcgca gccctcgcag aaggtcttgt ccacccgcgc
      901 cttgccgccc tccatcctta tggcgtcgaa gcggcagacg tcccggcact tgccgcagcc
      961 gatgcacctt tggctgtcga cgccggcggt gtccatcccg atgaaggcgt gctcctcccg
     1021 gggttcgggg tggaggagta tctccaggtc aggcgcgtcc acgtcggcgt cgcagagggc
     1081 cagccctccc ggggccgacg ccgccagggc cgccgttatg gaggtctttc cggtgccgcc
     1141 cttgccgctg accacggtta tctcccggat cttcatcttc ccgccatctc cttcgccctc
     1201 tcccacaggg tgaccatggc gtccctccag agggcgtcgg tcgccgcggg ggggttgccg
     1261 gcggcgtagg actccgccac ggccctcgaa aagggcagcc tcgccgccac ggggacaccg
     1321 tacctctccg tgaggccctc gatatcggcg tctcccaggt cgcagcggtt gacgaccgcc
     1381 atggccggtt tgcccagttc atggatcacc tgcaaagcca ggtcaaggtc ggcgtacccg
     1441 aagggcgtcg gctccgtcac caggaccacc acgtcggcct ccctcacggc cgacaccatg
     1501 gggcaggatg tccccggcgg ggaatcgatg accaggtagc taccctcggc gagggccgcc
     1561 ttcaaggtcg cgtcgatcac cggcacgggg ttgggcatcc ccacgtggag cctgccctcg
     1621 tagaggcgaa ggtcccccct gcggcagagc cttacctccc ccacgggcct ccctacttcg
     1681 gtgatggcct tctgggggca taccatggag cagaccccgc agccgtgaca gagatcgttg
     1741 ggcaccggcg ccccgccgcc gaacatcagg agggccccga agcggcaact cctgacgcac
     1801 tcaccgcatc ggttgcaaag gacattgtct atcaccggcc aggggatctc caccaggg
//
LOCUS       DAOSRG010000239         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_58076_length_1850_cov_11.055710, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000239 DAOSRG010000000
VERSION     DAOSRG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58076_length_1850_cov_11.055710"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1117)
                     /locus_tag="PLM30_06585"
     CDS             complement(392..1117)
                     /locus_tag="PLM30_06585"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOR54874.1"
                     /translation="MDLDLIAERISAGGLVILSGAGMSTASGLPDFRGADGLWRHKDP
                     RLLASVSAMENNRDEFVEFYRHRIRMLQGVEPNQGHRVLADWESRGLLRGVITQNVDG
                     LHQAAGSREVHELHGNLRKVSCARCRASFDSGLFLERDDCPDCGGPLRPGVVLFGEML
                     PEGPMEAAEDLSGASSSFLVLGSSLEVSPANAYPMLAKRAGATFFIINMTPTRLDPMA
                     DAVIHADIVATLEEIGKIVNQRS"
     gene            complement(1134..>1850)
                     /locus_tag="PLM30_06590"
     CDS             complement(1134..>1850)
                     /locus_tag="PLM30_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOR54875.1"
                     /translation="LYVFLRLKGDEGLGLRGWIDSFIVGAMLFLGGTGLLCVGQQWVA
                     SGLSATLLASTPLWTVLFAGIWDKMPLPMEWVGLGIGIVGVALLNFDKGISGNPMGAV
                     VILAASVIWSLGSVLNNRFKSLDKPIASATEMIAGGVLMLVVSALIGEKVSWPPTARA
                     SMGIFHLVVFSSIIGFTLYRYLLRTVRPALATSYTLVNPITATMLGALFAGESVSFIS
                     VAAMAVILSGVALVFRARNI"
BASE COUNT          364 a          588 c          567 g          331 t
ORIGIN      
        1 ctgcgaggag ttcttcatca ccggcagcgt gaaggagatc ctccccgtcg tcagggtggg
       61 aaggacgatc atcggcggcg gcaagccggg gccggtctca tcgcacctga ggaggcttta
      121 cctccagaac ctggaccgct ggctggagta aggaccgtga atcggggatc gttggtcgtg
      181 aatcggaaaa gccggatctt gtatcaccgg gataccctgt aataagccga gtattttttc
      241 ccttacgatc tgggcccggt tgcaactcgc tgaggccgga tcttacaaca aggactcgcc
      301 ccgtcgctga aaaactgcgg tacgaaatga cctggctttc ttttttcctt caccgttgac
      361 cccttacgtt tttcgccctt tggttcacgg ctcacgatct ttgatttacg attttgccta
      421 tctcctccag ggtcgccact atatctgcgt ggatcacagc gtcggccatg gggtccagcc
      481 tggtaggcgt catgttgatg atgaaaaatg ttgcccccgc cctcttcgcc agcatggggt
      541 aggcgttggc cggcgatacc tccagggagg aacccaggac aaggaaggag gaggacgccc
      601 cggagaggtc ttcggcggcc tccatggggc cttcggggag catctccccg aaaaggacca
      661 ccccgggcct caggggaccc ccgcagtcgg ggcagtcgtc ccgctcgagg aaaagcccgc
      721 tgtcgaagga cgcccggcag cgggcgcagc tgaccttcct gaggttcccg tggagttcat
      781 ggacttccct ggaacccgcc gcctggtgaa gcccgtcgac gttctgggtg atgacacccc
      841 tcaggagccc cctggactcc caatccgcca gcacccggtg tccctggttg ggctcgacgc
      901 cctggagcat cctgatcctg tgccggtaga actccacgaa ttcgtcgcgg ttattctcca
      961 tagcggagac ggaggcgagg agtcgcgggt ccttatgcct ccacaaaccg tcggcgcccc
     1021 tgaaatcggg aagccccgaa gccgtgctca tgcccgcccc cgacaggatg acgaggccgc
     1081 cggcggagat tctttcagcg atgaggtcca ggtccatatc gctcgctccc ttctcagata
     1141 ttcctggccc tgaagacgag cgccaccccc gagaggatca ccgccatggc ggccaccgag
     1201 atgaacgaaa cggactcccc cgcgaagagt gcccccagca tcgtggcggt gatggggttg
     1261 accagggtgt aactcgtcgc cagggccggc ctcaccgtcc tgagcaagta acggtagagg
     1321 gtgaacccta tgatcgagga aaagaccacc aggtggaata tgcccatcga tgccctcgcc
     1381 gtcggcggcc aggagacctt ctccccgatc agggcggaga cgacaagcat gaggaccccg
     1441 cccgctatca tctccgtggc cgaggctatg ggtttatcaa ggcttttgaa gcggttgttg
     1501 aggaccgacc cgagggacca gatgaccgag gccgcgagga tcaccacggc gcccatgggg
     1561 ttgccgctga tgcccttgtc gaagttgaga agggccacgc cgacgatccc tatcccgagg
     1621 ccgacccact ccatgggaag gggcatcttg tcccagatgc ccgcgaagag caccgtccac
     1681 aggggggtgc tggccagcag cgtagccgaa agtccggagg ccacccactg ctgccctacg
     1741 cagagaagcc cggtgccgcc caggaacagc atggccccca cgatgaagga atcgatccac
     1801 ccccggaggc ccaggccctc gtcgcccttc aggcggagga agacataaag
//
LOCUS       DAOSRG010000240         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_58389_length_1843_cov_13.991611, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000240 DAOSRG010000000
VERSION     DAOSRG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58389_length_1843_cov_13.991611"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLM30_06595"
     CDS             complement(<1..464)
                     /locus_tag="PLM30_06595"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR54876.1"
                     /translation="MWKDIKFVTPDIVFGMDAVNAVGDRIKALKGKRALVVTGPSVKA
                     SGALDKVLQSARDAGVDFDVNVQQRSTPEPTTELAEEVAKYIVDGKFDVVVGLGGGSI
                     LDVAKMSSALTTNPGKTRDYFGPGKVKNKGLPTIMIPTTAGTGSEVTKHAIFL"
     gene            689..1096
                     /locus_tag="PLM30_06600"
     CDS             689..1096
                     /locus_tag="PLM30_06600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR54877.1"
                     /translation="MAQIKIYNAETLGKPLGQYSHIARVKAGELLFISGMLSADGAGN
                     VVGKDDFDAQCTQVFKNIGEALASAGAGWGQIVQFTTYLVHSQDIPKFMEFRKREFPR
                     LFPDGVYPPNTLLMVDRLVQEPFLVEVQTIAAL"
     gene            complement(1178..>1843)
                     /locus_tag="PLM30_06605"
     CDS             complement(1178..>1843)
                     /locus_tag="PLM30_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54878.1"
                     /translation="AYMTLREGIITRRYRPGSPMIITNLSKSLGVSETPVRDAIKMLI
                     SEGFVVSNDHVGAKVASPSLEDLKNMFEMLLSLQGIATRMAVYQDPAGLLEELEEILE
                     KTKLYIHEQRHEEYGILNDRFHDSIESRCRNLELVSTINSYREKTGWVRSLFVLNPKR
                     LEESFDEHVEIFGALKERKARAAEKILKKHLLISFNGYLDFLKKEKGYEGKEYEIDGS
                     RWI"
BASE COUNT          340 a          564 c          514 g          425 t
ORIGIN      
        1 aggaagatgg cgtgcttggt gacctctgaa ccggtcccag cggtggtggg gatcatgatg
       61 gtggggagcc ccttgttctt gaccttcccg ggaccgaaat agtcccttgt cttgccgggg
      121 ttcgtggtga gcgccgagga catcttcgcc acgtcgagaa tactgccgcc gccaagcccc
      181 actactacgt cgaatttgcc gtcaacgatg tacttcgcca cttcctcggc gagttcggtg
      241 gtgggctcgg gtgtagaccg ctgctggacg ttgacgtcga agtccacgcc ggcgtccctg
      301 gcggattgca acaccttgtc aagcgctccc gaagccttca ccgaggggcc ggtgaccacc
      361 agggcccttt tccccttgag agccttgatc ctgtccccga cagcgtttac ggcgtccatg
      421 ccgaaaacga tatcgggggt cacgaattta atgtccttcc acatcttgaa aactctcctc
      481 tccttttctt tttcctgttt ttcttgatcg gactgtcctg tcgctttacg ggcggcccat
      541 tgctgtatac tatcttgcct cggggcgcac cttatgttat atacttgaaa agtactgccc
      601 gacagagtcg ataacgggcg cttcggccgc gggctccaag ggtccgcggg acccgaagcc
      661 caataaacga tagggggagg atgaaccaat ggcccagatc aagatctaca acgctgaaac
      721 cctggggaaa cccctgggac agtattcaca catcgcccgg gtcaaggccg gcgagctgct
      781 cttcatctcc ggcatgctct cggccgatgg ggccggtaac gtggtcggaa aagacgactt
      841 cgacgcccag tgcacccagg tcttcaagaa catcggtgaa gccctcgcct cggcgggtgc
      901 cggctggggc cagatcgtgc agttcacgac ctacctggtc cactcgcagg atatcccgaa
      961 gttcatggaa ttccggaaac gggagttccc gcgcctcttc cccgacgggg tctacccgcc
     1021 taacaccctt ctgatggttg acaggctcgt ccaggaaccc ttcctcgtcg aggtccagac
     1081 tatcgccgcc ctgtaacgga accgtcggac gtctcggggc tccccgccgc cacaagggcg
     1141 gggagtcctt ttaggggccc gaaagggccc tgccagttca gatccagcgg cttccgtcta
     1201 tctcatactc cttcccctcg taccccttct cttttttcag gaaatcaagg tagccgttga
     1261 aggatatgag caggtgtttt ttcaggatct tttccgccgc cctggctttt cgctccttga
     1321 gggcgccgaa aatctcaacg tgctcatcaa aggattcctc gaggcgtttc gggttcaaca
     1381 cgaagaggga gcgcacccac ccggtctttt ccctatagga gttgatggtc gagaccagtt
     1441 caaggtttcg gcaccggctc tcgatagaat catggaaccg gtcattcagg atcccgtatt
     1501 cctcgtggcg ctgctcgtga atgtagagct tcgtcttctc caggatctct tccagttcct
     1561 caaggaggcc cgccgggtcc tggtacaccg ccatcctcgt ggcgatgccc tggaggctca
     1621 ggagcatctc gaacatgttc ttcaggtcct ccagggaggg gctggccacc ttcgctccca
     1681 cgtgatcgtt ggagaccacg aacccttccg aaatgagcat ctttatggca tccctgacgg
     1741 gcgtctcgct cacgcctagg cttttgctga ggttcgtgat tatcatcggg gacccgggac
     1801 ggtaacgacg ggtgatgatc ccttccctaa gggtcatgta ggc
//
LOCUS       DAOSRG010000241         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_58393_length_1843_cov_6.700783, whole genome shotgun sequence.
ACCESSION   DAOSRG010000241 DAOSRG010000000
VERSION     DAOSRG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58393_length_1843_cov_6.700783"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLM30_06610"
     CDS             <1..1037
                     /locus_tag="PLM30_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54879.1"
                     /translation="GGYGTGRELVEYFGNFGSLGGILGMALVTTTLWAVVYAASYEFA
                     RTFKVYDYRGFFKELLGPGWMLYEVCYLVLLLIVLGVVGAASGSIFKQAFGLPPLVGA
                     ALFLAGVVTLTFFGSYVIEVALSWWSFVLYAVFLIFLFVGLSKVGGQISANLASGDIR
                     EGWALGGFKYAFYNLGTLPAVLFALNYIETRREAIISGILTSILTILPGFFLYLVLMG
                     FYPGILEAELPAYTILSQIAPWLLPIFLVVLFGTMIETAVGFIHAVNERVNSWMVDRR
                     NSELTKANRGVLGGVMGLIGLGVASFGLIGLIAKGYGTISWGFFLLHGVALFTIGLYK
                     MSKKGGKASA"
     gene            1066..>1843
                     /locus_tag="PLM30_06615"
     CDS             1066..>1843
                     /locus_tag="PLM30_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR54880.1"
                     /translation="MDWRSLAGLTGKNIEKSCAVIEGEERVIPRAVSIKYFPMAIEKA
                     QGSLVWDADGNRYIDFLTSAAAYNAGHCHPAVVEAVKEQVERLQNYTMVYFYNDRPVR
                     LAELLITTTPGDAPKKAVFGFSGSDGVDAAIRAARAFTGRRQILSFKESYHGMTTAAL
                     SVTGIVSDEAKKDVCLDPHVIFAEYPDPYRNSWGIDGYADPAALSGKAMESIAALLER
                     HRGDVAAVLIEPAQGDGGMIFPPGEFMTRLREVTEREGIVL"
BASE COUNT          328 a          586 c          560 g          369 t
ORIGIN      
        1 ccgggggtta cggcacaggt cgtgaactcg tcgagtactt cgggaatttc ggcagtctcg
       61 gcgggatcct cggcatggcg ctggtgacta cgaccctgtg ggccgtcgtc tacgccgcca
      121 gttacgaatt cgccaggacc ttcaaggtat acgactatcg gggtttcttc aaggaactcc
      181 tcgggcccgg ctggatgctc tatgaagtgt gttacctcgt gctcctcctt attgttctcg
      241 gcgtcgtcgg ggccgcctcg gggagcatct tcaagcaggc cttcgggctg ccgcccctgg
      301 tgggtgctgc cctcttcctg gcgggcgtcg tcacgctgac cttcttcggc agttacgtca
      361 tcgaggtcgc cctctcgtgg tggtccttcg tcctctacgc ggtcttcctg atcttcctct
      421 tcgtggggct ctccaaggtg ggaggccaga taagcgcgaa cctggcttcc ggggacatcc
      481 gggaaggctg ggccctggga ggtttcaaat atgcctttta taacctgggt accctcccgg
      541 ccgttctctt cgccctcaat tacatcgaga ccaggagaga ggccatcatc tcggggatat
      601 tgacgtcgat cctgacgatc ctacccggtt tcttcctcta ccttgtcctg atgggcttct
      661 accccggcat cctcgaagcg gagctgcccg cctacaccat cctgtcgcag atcgccccct
      721 ggctgctgcc gatcttcctg gtggtccttt tcgggacgat gatcgagacg gccgtcggct
      781 tcatccacgc cgtcaacgaa cgggtcaact cctggatggt ggaccggagg aactcggaac
      841 tgaccaaggc caaccggggc gtcctcgggg gggtcatggg cctgatcggc ctcggcgtgg
      901 cctccttcgg cctcatcggc ctcattgcca agggttacgg gaccatcagc tggggctttt
      961 tcctgctcca cggcgtggcc ctcttcacga taggtctgta caagatgagc aaaaagggcg
     1021 ggaaggcatc cgcctgatcc cgcggcgagg ggaggatgcc cgcagatgga ttggagatcc
     1081 ctggccgggt tgaccggcaa gaacatcgaa aaaagctgcg ccgtcatcga gggtgaagag
     1141 agggtgatac cccgggccgt gtcgataaaa tacttcccca tggccatcga aaaagcccag
     1201 ggctccctcg tctgggacgc cgacgggaac aggtatatcg acttcctgac cagtgccgcc
     1261 gcctacaacg cgggccactg ccacccggcg gtggtcgagg ccgtcaagga gcaagtggaa
     1321 cgtctccaga attacacgat ggtctatttc tacaacgacc ggccggtaag gctcgccgaa
     1381 ctcctgatca ctacgacccc aggggacgca cccaaaaagg ccgtcttcgg cttctccggg
     1441 tccgacggcg tcgacgccgc catcagggcc gcccgagcct tcaccgggcg caggcagatc
     1501 ctcagcttca aggaatccta ccacggcatg accacggccg ccctttcggt gacgggcatc
     1561 gtcagcgacg aggccaaaaa ggatgtctgc ctcgaccccc acgtgatctt cgccgagtat
     1621 cccgatccct accgcaattc ctgggggatc gacggctacg ccgaccccgc agccctttca
     1681 ggcaaggcta tggagtccat cgcggcactc ctggaaaggc atcgcggcga cgtggcggcc
     1741 gtcctgatcg aaccagccca gggtgacggg gggatgatct tccccccggg ggaattcatg
     1801 acccggctca gggaggtcac ggaaagggaa gggatcgtgc tca
//
LOCUS       DAOSRG010000242         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_58827_length_1834_cov_6.271501, whole genome shotgun sequence.
ACCESSION   DAOSRG010000242 DAOSRG010000000
VERSION     DAOSRG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58827_length_1834_cov_6.271501"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1171)
                     /locus_tag="PLM30_06620"
     CDS             complement(161..1171)
                     /locus_tag="PLM30_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOR54881.1"
                     /translation="MSRAGLHVISHTDLDGVTSALVAWTYARPRMSPVRVSLAGYGEV
                     DLLVMDSLSRGEPFVVLDLFCQRPQTVDEIDRTFVQGDPPLFFDHHQSTLERYGNRPW
                     VQVDVKSCAALVYYRWLMEKATGEDRRRLARLEGIVETANDRDLWLNRIPESRLWQAL
                     VTLCGPWAVLARLIEDPSPGLEPDERAFTEDFVREQEARFGLALEKALRSGEDLLFVG
                     PGSLEFGDVSDFCGLVLDRMASPPRLVAVLSRRPAGDWSVSLRSREGFAGRVTGLLRD
                     GKKVRGGGHDDSAGLYFPPQYRSEEIRDSLLSAVRSALESDAPSGLTLGDLLKQAMKE
                     KE"
     gene            1486..>1834
                     /locus_tag="PLM30_06625"
     CDS             1486..>1834
                     /locus_tag="PLM30_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HOR54882.1"
                     /translation="MQEKTDQVWREILGIAEESLPKGAADIWLKTCLPVSLEGKVLTL
                     DVPNVFVREQIQSRFIEKLTALSSQRGLADKIVLEVGGAKKDEIKRAERISREAERPR
                     NGLNPDYQFDSFVV"
BASE COUNT          373 a          577 c          563 g          321 t
ORIGIN      
        1 gcagggcgaa ggagagtgtg tccaggcaga ggacccgcga gtcgggcacc tcccggccga
       61 accatttctc cagggccagg gccgcactcc tgtggccggt cccgaccgaa gcgtacagaa
      121 gagccgctcg aaatgacaag ggcctcaccc tccccggatc tcactccttt tccttcatgg
      181 cctgtttaag caggtcgccc agcgtcagcc ctgagggcgc gtcggactcc agggcggatc
      241 tcaccgccga taagagcgaa tcgcggatct cttcggatct gtactggggc gggaagtaaa
      301 gccccgccga gtcgtcgtga ccgccccccc ggaccttttt gccgtcccga agcagccccg
      361 tcacccttcc cgcgaagccc tccctgctcc tgagggagac gctccagtcg cctgcggggc
      421 gccggctgag gaccgccacg agcctggggg gcgacgccat ccggtccagg acgaggccgc
      481 agaagtccga gacgtcgccg aactcgaggg aacccggtcc gacgaagagc aggtcctcac
      541 cggatcgcag cgccttttcg agggcgaggc cgaatcgcgc ttcctgctcc cggacgaagt
      601 cctctgtgaa ggctctctcg tcgggctcaa gccccggaga ggggtcctcg atcaggcggg
      661 ccaggacggc ccacgggccg cagagggtga ccagggcctg ccacagcctg ctctcgggga
      721 tccggttaag ccagaggtcg cggtcattgg cggtttccac gatcccctcc agtcgggcca
      781 gccgccgccg gtcctccccg gtggccttct ccatgagcca ccggtaatag accagggccg
      841 cgcagctttt cacgtcaacc tggacccagg gcctgttccc gtaccgttcc aacgtgctct
      901 ggtggtgatc gaagaacagg ggaggatccc cctggacgaa ggtgcggtcg atctcgtcca
      961 ccgtctgggg cctctggcag aagagatcaa gcacaacgaa gggctcgccc ctggagaggg
     1021 aatccatcac caggaggtcc acctcgccgt aacctgccag ggagaccctg acgggactca
     1081 tcctggggcg ggcgtaagtc cacgccacga gggccgatgt cacgccgtcg aggtcggtat
     1141 gacttatcac gtgaaggccg gcacggctca tcgagaatct ccttcccggc ccgaaggtca
     1201 gcgcccagca gggcggcgtc gggctaccgg tattataggc gagaaatcca caccccatgt
     1261 gaacaggaat gcgaacaact ttttttgccc gaaaggagcg gtttcgttac ggttggtcaa
     1321 atagtacttt tttacgacca ctttgaaaac acccatcaac cgacgggttt tccctcttat
     1381 ctctacatta tgaactaaat gtggatatgt ggataaaact tgtggattca aatatggtaa
     1441 tattgacttc gccatcggtc caaaaaggtc gtgaggtgag cccccttgca ggaaaaaacc
     1501 gatcaggtct ggagagagat ccttgggata gccgaggaga gtctgcccaa gggtgccgcg
     1561 gacatctggc tcaagacgtg cctccccgta tccctggagg gcaaggtcct cacgctggac
     1621 gtccctaacg tcttcgtgag agagcagatc cagtcgaggt tcatcgaaaa actcaccgcc
     1681 ctctcctctc agagggggct ggccgacaag atcgtcctgg aagtgggggg cgccaagaag
     1741 gacgagatca agcgggccga gaggatctcc agggaagcag agaggcccag gaacggcctc
     1801 aaccccgatt accagttcga ctccttcgtg gtgg
//
LOCUS       DAOSRG010000243         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_59063_length_1829_cov_10.534949, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000243 DAOSRG010000000
VERSION     DAOSRG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59063_length_1829_cov_10.534949"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLM30_06630"
     CDS             complement(<1..769)
                     /locus_tag="PLM30_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOR54883.1"
                     /translation="MGGKGRLVIVSNRLPVKIEGHPGKWKVTHSSGGLVSALTPVLRD
                     RGGIWIGWAGTTEVLDLKTLREILGPASKQAGYRIYPVRLSREDVDSFYHGFSNEVIW
                     PLFHDLQTRCRFEPEYWKSYRKVNGDFAKAIKKHTRPEDYIWVHDYQMVPVAKILRSS
                     EVQRNCVFFLHIPFPPPDIFLKLPWREEFLKDFLEYDFVGFQTMRDRRNFTDCLKALL
                     PDSRVTGRGPVATVTAGGRRVRVAAMPISIDYRSFRDT"
     gene            complement(786..>1829)
                     /locus_tag="PLM30_06635"
     CDS             complement(786..>1829)
                     /locus_tag="PLM30_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR54884.1"
                     /translation="SPDFFTVTKKMHNALQGDEINLSWKKFRIYEHVVFENVLRNHLD
                     HDIVVIHDPQPLPMIRHYRKKGPWVWVCHVDLSNPNRKLWPYIRNYVNRYDAAVFSIP
                     EYRQDTKTPLVFIKPAIDPFSIKNRDFSEEEVQERLERYGIPTDKPLVVQVSRFDRWK
                     DPGGVIEAFKIARKEVDCNLVLLGNIATDDPEGVEIFNELKEKQEEGIIMLTVEDTAL
                     VNALQRKAAVVIQKSIREGFGLTVTEAMWKGKAVIGGNVGGIRHQITDGENGFLVDTV
                     EEAAERIVMLLKDPGLRDRVGEKARATVKEKFLMSRYLEDYLDLFNSFEANYTLREPL
                     WDRRTRPWAGRRR"
BASE COUNT          312 a          560 c          555 g          402 t
ORIGIN      
        1 ccgtatccct gaaggacctg tagtcgatgc tgatgggcat ggccgccacc ctgacacggc
       61 gccccccggc ggtgacagtg gcgaccgggc cgcggcccgt taccctggag tccggcagga
      121 gggccttgag gcagtcagtg aagttccttc tgtcgcgcat ggtctggaaa cccacaaagt
      181 cgtactcgag gaaatccttc aagaactcct cgcgccacgg cagtttcagg aagatgtccg
      241 ggggtgggaa ggggatgtgg aggaagaaga cgcagttcct ctggacctcc gatgatctca
      301 gtattttcgc gacggggacc atctggtagt cgtggaccca gatgtaatcc tccggtctcg
      361 tgtgcttttt tatagccttg gcgaaatcgc cgttcacctt tctgtaggac ttccagtact
      421 ccggttcaaa acggcatcgc gtctgaaggt cgtggaagag gggccagatc acctcgttgg
      481 agaacccgtg gtagaaggaa tccacatcct cccgggaaag ccgcacgggg tagatcctgt
      541 acccggcctg cttcgaagcg gggccgagga tctcccggag ggtcttcaag tcaagcacct
      601 ccgtggtccc ggcccagcct atccatatac cccccctgtc acggaggacc ggcgtcagcg
      661 ccgaaacgag cccccccgag gagtgggtga ccttccattt gcccgggtgc ccctcgatct
      721 taaccgggag ccggttcgat acgatgacca gtcttccctt gccgcccatg gatcgccccc
      781 gccggttacc ggcgtcttcc ggcccagggc cgggttctcc tgtcccagag cggttcgcgg
      841 agggtgtagt tcgcctcgaa ggaattgaag aggtcgaggt agtcctcgag gtagcgggac
      901 atgaggaact tctccttgac cgtggcgcgg gctttttcgc cgacccggtc gcgcaggccc
      961 gggtccttca ggagcatcac gatcctctcg gcggcctcct cgaccgtgtc gaccaggaaa
     1021 ccgttctcac cgtcggttat ctggtgcctt attcctccca cgttgccccc aatgaccgct
     1081 ttgcccttcc acatggcctc cgtcacggtc aggccgaaac cctcccgtat cgatttctgg
     1141 atcaccaccg ccgccttgcg ttgaagggcg ttcaccaggg cggtgtcctc gacggtgagc
     1201 atgattatgc cctcttcctg cttctccttc agttcgttga agatctccac gccctcgggg
     1261 tcgtcggtgg ctatgttgcc cagcaggaca aggttgcagt cgacctcctt gcgggctatc
     1321 ttgaaggcct cgatcacgcc ccccgggtcc ttccagcggt cgaaccggga cacctggacc
     1381 accaggggct tgtcggtggg tatcccgtac ctttcaagcc tctcctggac ctcctcctcc
     1441 gagaagtccc ggttcttgat gctgaagggg tcgatggcgg gcttgatgaa gaccaggggg
     1501 gtcttcgtgt cctgcctgta ctcgggtatg ctgaacacgg ccgcgtcgta gcggttgaca
     1561 tagttcctga tgtagggcca cagcttcctg ttcgggttgg aaaggtccac gtggcagacc
     1621 cagacccagg gccctttctt gcggtaatga cggatcatcg ggaggggctg cgggtcgtgg
     1681 atcacgacta tgtcgtggtc caggtggttc cggaggacgt tctcgaagac cacgtgctcg
     1741 tatatcctga acttcttcca actgaggttg atctcatcgc cctggagggc gttgtgcatc
     1801 ttcttcgtga cggtgaaaaa gtcggggga
//
LOCUS       DAOSRG010000244         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_59220_length_1826_cov_9.544890, whole genome shotgun sequence.
ACCESSION   DAOSRG010000244 DAOSRG010000000
VERSION     DAOSRG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59220_length_1826_cov_9.544890"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..166)
                     /gene="rpmH"
                     /locus_tag="PLM30_06640"
     CDS             complement(32..166)
                     /gene="rpmH"
                     /locus_tag="PLM30_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR54885.1"
                     /translation="MKQTFQPHNRRRKRRLGFLQRSSSPSGRRILQNRRNKGRKRLAV
                     "
     gene            complement(323..1213)
                     /gene="rbsB"
                     /locus_tag="PLM30_06645"
     CDS             complement(323..1213)
                     /gene="rbsB"
                     /locus_tag="PLM30_06645"
                     /inference="COORDINATES: protein motif:HMM:NF007936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HOR54886.1"
                     /translation="MKGRRLLSVAMAVLLVLSFPLAAAASQTLGVVISTLNNPFFVTL
                     KDGAEEKAKELGVELVIYDAQDDSAKMTSSMEDLIQKKVGAILVNPTDADAVVPSILK
                     ANAAGIPVFTIDRGATSGEVVTHIASDNVAGGKMAGEFLAEKIGGKGKVVELVGIPGT
                     SAARDRGKGFHDAMEGYPDIEIVASQAADFNRDRGLAVFENILQAQPEIDGVFAHNDE
                     MVLGAIAAAEAAGRKGIVFVGFDAVDDAVKAVKDGRLAATVAQQPKEMGLLGVEYAVK
                     YLEGEAIPDSVPVPLMLVTE"
     gene            complement(1263..>1826)
                     /locus_tag="PLM30_06650"
     CDS             complement(1263..>1826)
                     /locus_tag="PLM30_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR54887.1"
                     /translation="AARGAALLFTGGAPISGFPEAFRLPGTGWFAGVPLPVLVFAGVF
                     AALFVLLELSSWGEQVRSIGSNPVAAWGSAIPVPAITASVFVLSGVLAGLAGLVLAGR
                     LDAAQPTAGLGYEFGAIAAAVLGGSQFSGGKGTLFGTIMGVLIMGVIGNGINVLNVNP
                     FYEQVVRGLVIAAALVFYGRVSAGNPA"
BASE COUNT          333 a          620 c          547 g          326 t
ORIGIN      
        1 cctgggaagg gaaaatgaaa aaagcccgca actagaccgc caggcgcttt ctgcccttgt
       61 ttctcctgtt ctggaggatg cgccggccgc ttgggctgct cgatcgctgc aggaacccga
      121 gtcttctctt ccttcttcta ttgtgcggct ggaaggtctg tttcaattga tacacctcct
      181 cttgctatcg cgtgggaact ccgggaaaaa accagcccgg ctactataac ataccgggtt
      241 atgggcaaca aggggccccc agggagcggg gaaaaacctg gatttagtca aaagtgtaga
      301 cctgaccccg ctccctgggt tgctattcag ttacgagcat gagggggacg gggacgctat
      361 cggggatggc ctcaccctca aggtacttga cggcgtactc gacgcccagg agccccatct
      421 ccttcggctg ctgggccacg gtggcggcca gcctgccgtc cttcacggct tttacggcgt
      481 cgtccacggc gtcgaaaccg acgaacacga tgcccttccg tccggccgcc tcggcggcgg
      541 cgatggcccc gagcaccatc tcgtcattgt gggcgaagac cccgtcgatc tcgggttgcg
      601 cctggaggat gttctcaaag acagcgagcc ccctgtcgcg gttgaagtcc gccgcctggg
      661 aggccacgat ctctatgtcg ggatacccct ccatcgcgtc gtggaatccc ttgcccctgt
      721 cacgggcggc cgacgtgccg gggatgccca ccagctccac caccttgccc ttgccgccga
      781 tcttttccgc caggaactct cccgccatct tgcccccggc gacgttgtcg ctggcgatat
      841 gggtgaccac ctcacccgag gtcgcccccc ggtcgatggt gaataccggt atgcccgccg
      901 cgttggcctt caggatcgac ggcaccaccg catcggcgtc agtggggttc accagtatgg
      961 cccccacctt cttctggatc aggtcctcca tggaagaggt catcttggcc gagtcgtcct
     1021 gcgcgtcgta gatgaccagt tccactccga gttccttggc cttctcctcg gccccatcct
     1081 tgagggtcac gaaaaaggga ttgttgagcg tggagatgac cacgcccagg gtttgagagg
     1141 ccgcagccgc aagggggaag gaaaggacca gcagaaccgc catggccact gacaacaagc
     1201 gccttccttt catcgctttc cacctccatt gattttatcc ttcccggccc gtcatagttc
     1261 gactaggccg ggttcccggc cgacacccgg ccgtaaaaga cgagggccgc cgctattaca
     1321 aggcccctta caacctgctc gtagaaaggg ttcacgttca ggacattgat gccgttcccg
     1381 atgaccccca ttatcaggac ccccataatg gtcccgaaga gggtcccctt cccgccggag
     1441 aactggctcc ctcccaggac ggcggcggcg atggccccga actcgtaacc cagccccgcc
     1501 gtgggctggg cggcgtcgag gcgccccgcc agcaccaggc ccgccagccc ggcgaggact
     1561 ccgctaagga cgaagaccga cgccgtgatc gccgggaccg gtatggcgga cccccaggcg
     1621 gccacggggt tggagcctat ggagcggacc tgttcgcccc aggacgatag ttcgagcagg
     1681 acgaaaaggg ccgcgaaaac gccggcgaag accagcaccg gcagggggac cccggcgaac
     1741 cagcccgtcc cgggcaaccg gaaggcctcg gggaagccgc tgatgggggc tccccccgtg
     1801 aaaagcagcg ccgcccccct ggccgc
//
LOCUS       DAOSRG010000245         1824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_59321_length_1824_cov_4.553985, whole genome shotgun sequence.
ACCESSION   DAOSRG010000245 DAOSRG010000000
VERSION     DAOSRG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59321_length_1824_cov_4.553985"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLM30_06655"
     CDS             complement(<1..379)
                     /locus_tag="PLM30_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR54888.1"
                     /translation="MLKKILVCVDMTRMADHLIEYGHTLAHRLGAEVTFINVLPSSLI
                     WKGYGTWVNPDWSKEAEESARKKIRYLIKLAEDKHPDLPKDEHEILVAQGNSAEVIIE
                     TAKKNDFNLIVIGFKSVSGIPQLM"
     gene            485..>1824
                     /locus_tag="PLM30_06660"
     CDS             485..>1824
                     /locus_tag="PLM30_06660"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOR54889.1"
                     /translation="MGPSSLFNHVLGPVMHGPSSSHTAASFHIGRIARSALGESPVEA
                     DIAFDPAGAYAEVYRQQGSDRAFAMGIMGRGITDPVYKNALEAVRGEGVDLTFTIKSL
                     KDKDHPNAVEVFLKGASGRKVILGARSVGGGAVEVTSLDGIGLSLRSDAWDLVVLAEE
                     GALPLLRSEAGALTLAGSSLREWGHEELIVFHAPCLEKPPAGLLEGLRLAGGVRELWL
                     LEPVFLPSKGEPLFSDASAMVSYAGREGVTLGEAGLRYEAALLGFSREEAMNEMKRRY
                     RVMEESVRAGLSGEGLSMRLMDPVAGGIMEAESRGGLALGGLHTRAAARALAAMHVNA
                     SGGLVVAAPTAGSAGTIPGVAVTLRQEMGLGEEEVARVLFAASCIGLVISNRATFAAE
                     VAGCQVEIGAAGAMAAAGVVEYAGGNAAQACDAAAIFLQNWMGSVCDPVQGFVE"
BASE COUNT          289 a          554 c          639 g          342 t
ORIGIN      
        1 ccatcaactg cggtatgccg ctgacgctct tgaagcctat gacgatcagg ttgaagtcgt
       61 tttttttcgc cgtctcgatg atgacctcgg cggaattgcc ctgggcgacc aggatctcgt
      121 gctcatcctt cggcaggtcg gggtgcttgt cctcggccag cttgatgagg taacggatct
      181 tcttgcgggc gctctcctcc gcctcctttg accagtcggg gttgacccag gttccgtacc
      241 ccttccagat gagcgagctg ggaaggacgt tgatgaaggt cacttccgcg cccaggcggt
      301 gggccagggt atgcccgtat tcaatgaggt gatcggccat cctggtcatg tcaacgcaga
      361 cgaggatctt cttcagcatg atgacccctc ctgtctttgg tcttgtacaa ggattatacg
      421 ataaggatac cttatactgg tcaccacagg tgttgcagca cctgtaccgg aaggagggtc
      481 ccccgtgggt ccgtcaagcc tgttcaacca cgtccttggt cccgtcatgc acggcccgtc
      541 gagctcccac acggcggcgt cgttccatat cggcaggatc gcccggtccg ccctgggaga
      601 gagccccgtc gaggcggaca tcgcctttga tcctgccgga gcttacgcgg aggtgtaccg
      661 gcagcagggg agcgacaggg ccttcgccat gggcatcatg ggccggggta taaccgaccc
      721 cgtttacaag aatgcccttg aggccgtccg aggagaggga gtcgacctca ccttcacaat
      781 aaaatccttg aaggacaagg atcaccccaa cgccgtggag gtgttcctca agggcgcgtc
      841 gggcaggaag gtcatcctgg gcgcccggtc cgtgggaggc ggcgccgtgg aggtcacctc
      901 cctggacggc atcggtctct ccctgcgaag cgacgcctgg gacctggtgg tcctcgccga
      961 ggaaggcgcc ttgcccttgc tgaggtcgga ggcgggggcg ctgaccctgg ccggctcctc
     1021 cctccgcgag tggggccacg aggagctgat cgtttttcac gccccctgcc ttgaaaagcc
     1081 cccggcgggg ctgctcgagg ggctgcgcct cgccggcggc gtgagggagc tgtggctcct
     1141 ggaacctgtc ttcttaccct ccaaggggga gccccttttc agcgacgcct cggcgatggt
     1201 ctcctacgcc gggcgggaag gggtgaccct cggcgaggcg gggctgcgct acgaggcggc
     1261 gctgctggga ttctcccgcg aagaggccat gaatgaaatg aagaggcgtt accgggtaat
     1321 ggaagaatcc gtgagagcgg ggctttccgg cgaaggcctg tccatgaggc tgatggaccc
     1381 cgtcgccggg ggcatcatgg aggccgaatc ccggggcggc ctggccctcg gggggcttca
     1441 caccagggca gcggcgaggg ccctggccgc catgcacgtg aatgcctcgg gggggctggt
     1501 cgtggccgcc ccgacggcgg gttccgccgg cacgataccg ggcgtggcgg tgacattgag
     1561 gcaggagatg ggcctcgggg aggaggaggt cgccagggtg ctcttcgcgg cctcctgcat
     1621 agggctggtg atatcgaaca gggctacctt cgccgccgag gtggcggggt gccaggtgga
     1681 gatcggcgcc gccggggcta tggccgccgc cggggtggtg gagtacgccg ggggaaacgc
     1741 cgcccaggcc tgtgacgccg ccgccatatt cctccagaac tggatgggct ccgtctgcga
     1801 ccccgtccag ggtttcgtgg agat
//
LOCUS       DAOSRG010000246         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_59767_length_1814_cov_8.498010, whole genome shotgun sequence.
ACCESSION   DAOSRG010000246 DAOSRG010000000
VERSION     DAOSRG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59767_length_1814_cov_8.498010"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLM30_06665"
     CDS             <1..285
                     /locus_tag="PLM30_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54890.1"
                     /translation="HLQGTGKTSVSLEEVVPASAQGRVDTLFVALGRQAWGTFDVESG
                     VIELQEAHAFGNRDLLDLAAVLTIKGGGKVYAVALDEVPGGFDLAAILRY"
     gene            490..>1814
                     /locus_tag="PLM30_06670"
     CDS             490..>1814
                     /locus_tag="PLM30_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR54891.1"
                     /translation="MKEYDLMVIGMGPAGMAISAMGSAMGLSVVSFEKHKVGGECLNC
                     GCIPSKALLKAAEAFHSARNLEKYGIIAGLSVDDFDPMGVVREKINGITGKKMMSVFE
                     KVHLVQGEAEFVDDRTVRSGGEDYRAKKIFIAVGTEPFIPPIPGLRDIPDILTNANLF
                     QLEKMPESLTIIGGGAIGSEMAQAFTRLGTKVSMIHMDPHLIPLGDAEAAQLLEESFR
                     SEGIIVHNGAAIESVSLEDGLVVVKAGDILLKSQRILVAAGRKPALEPLKLDKAGIEF
                     TKKGITVDSYMRTNKRHIYATGDCNGRNLFSHAAMHQGMLALMHALSPLSLPFLKRGR
                     YAVPWSVFTEPEVAQVGLTEKQAREKGLRVEIAKKDFRTYGRTVADGHPDGFIKIVTD
                     PRGRIHGATIVGENASELIHEWTMAIQYKKKMHHVMMMQHSFPSVSMIN"
BASE COUNT          390 a          610 c          515 g          299 t
ORIGIN      
        1 cacctccagg ggacgggcaa gacctccgtc tccctcgagg aagtcgtccc cgcctctgcc
       61 caggggaggg tcgacaccct cttcgtcgcc ctgggcaggc aggcctgggg gaccttcgac
      121 gtcgaaagcg gcgtcataga actgcaggaa gcccacgcct tcggcaaccg cgaccttctc
      181 gaccttgcgg ccgtcctcac catcaagggg ggcggcaagg tctacgccgt cgccctcgac
      241 gaagtccccg gaggcttcga cctcgccgcc atcctccgtt actgattggg gtcagacctt
      301 gaatttgatc atttgtatac ataaaggtaa aaattcgggc gcccgccgtc aggtgtcttc
      361 cgcctctttg tacacaaatg ctcaaattca aggtctgacc ccaaagcttg actctatacc
      421 gatagggggt ataatgggga cgctaaatta aaaatatcac gatctccatt ccaggggagg
      481 gcggctagga tgaaagaata cgacctcatg gtcatcggca tgggccccgc ggggatggcc
      541 atatcggcca tgggttccgc catggggctc tcggtggtct ccttcgaaaa gcacaaggtc
      601 ggcggcgagt gcctcaactg cggctgcatc ccaagcaagg ccctcctgaa ggcggctgag
      661 gccttccact cggccaggaa cctggaaaaa tacggcatca tcgccgggct ctccgtcgat
      721 gacttcgacc ccatgggcgt cgtccgagag aagattaacg gcatcaccgg caagaagatg
      781 atgagcgtct tcgagaaggt ccacctggtc cagggcgagg ccgagttcgt cgacgaccgc
      841 acggtccgct ccggcggcga ggactaccgg gcgaagaaga tcttcatcgc cgtcggaacg
      901 gagcccttca tcccgcccat ccccggcctc agggacatcc ccgacatcct caccaacgcc
      961 aacctcttcc aactcgagaa gatgcccgaa agcctcacca tcatcggcgg gggagccatc
     1021 ggcagcgaga tggcccaggc cttcacccgc ctcgggacga aggtctcgat gatccacatg
     1081 gacccccacc tcatccccct gggcgacgcc gaggctgccc agctcctgga ggagtccttc
     1141 cggagcgagg gcatcatcgt ccacaacgga gcggccatcg agagcgtctc cctcgaggac
     1201 ggcctcgtgg tcgtcaaggc cggcgacatc ctgttgaagt cccagcgcat cctcgtcgcc
     1261 gccggccgga agccggccct ggaacccctg aaactggaca aggccggcat cgaattcacc
     1321 aaaaagggca tcaccgtcga cagctatatg cgcaccaaca agcgccacat ctacgcgaca
     1381 ggcgactgca acggccgaaa cctcttctcc cacgccgcca tgcaccaggg catgctcgcc
     1441 ctgatgcacg cactgtcgcc cttatcgttg cccttcctca agcgggggcg ctacgcggtg
     1501 ccctggtcgg tcttcaccga acccgaggtg gcccaggtgg ggctcaccga aaagcaggcc
     1561 cgggagaagg gcctccgggt cgaaatagcg aaaaaggact tccgcaccta cggccgcacc
     1621 gtggccgatg gccaccccga cggcttcata aaaatcgtca ctgaccccag ggggcgcatc
     1681 cacggggcca ccatcgtggg cgagaacgcc agcgaactca tccacgaatg gaccatggcc
     1741 atccagtaca aaaagaagat gcaccacgtg atgatgatgc agcactcctt cccgtcggtc
     1801 tcgatgatca acaa
//
LOCUS       DAOSRG010000247         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_61043_length_1788_cov_7.345066, whole genome shotgun sequence.
ACCESSION   DAOSRG010000247 DAOSRG010000000
VERSION     DAOSRG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61043_length_1788_cov_7.345066"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLM30_06675"
     CDS             complement(<1..641)
                     /locus_tag="PLM30_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54892.1"
                     /translation="MTLYDVLVIDAQNDFCKPGGALFVPGAPGDTERVCALLDRLDRK
                     GSIGNYHLTMDTHYVLDISHPGFWRDPEGNLPEPYTQITAADLASGKWLPKDPGSLER
                     VRLYLERLDASGRYTHTIWPEHCILGSPGHCLDEEVLERVNRWERREGRRADYILKGM
                     NLWTEHYSVLKAEVEDPDDIGTALNRRLLGELSLARAVIVSGQALSHCVANTIT"
     gene            complement(756..1739)
                     /locus_tag="PLM30_06680"
     CDS             complement(756..1739)
                     /locus_tag="PLM30_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOR54893.1"
                     /translation="MTEKVKEVNVAIIQEPPVFLNLGASVEKACALTRKAAGDGARVV
                     VFPETWLPGYPVWIDSAPEAALWGHDAAKGLFRRLVENALTLQGDETAALARAAVESK
                     VHMVMGAHELVGNTLYNTMLFISPDGVYSAHRKLTPTYTERLLWGMGDGSTLVSVDAP
                     FGVLGGLICWEHWLPLARAAMHARKETVHVAQWPWVRDLHLVASRHYAFEGQCFVLAA
                     GTVLTVGDVLEGYDSLPGAIQECREMLSSMGNPAELLQRGGSTVIAPDSTCLVEPLYD
                     AVGTVHATLDLGMVTEGHLLMDSDGHYSRPDVFTLTVNTAPLRGVRFEPRQ"
BASE COUNT          329 a          578 c          530 g          351 t
ORIGIN      
        1 gtgatggtat tggccacgca gtgactcagg gcctggcccg acactatcac cgcccgtgcc
       61 aaggaaagtt cgcccagcag ccgcctgttc agcgcggtgc cgatgtcgtc gggatcctcc
      121 acttcggcct tcagcaccga gtagtgctcg gtccagaggt tcatcccctt gaggatataa
      181 tcggccctcc tcccttcccg ccgttcccac cggttaaccc tctcgaggac ttcctcgtcg
      241 aggcagtgcc cgggcgagcc gaggatgcaa tgctcgggcc agatagtatg ggtatacctt
      301 cccgaggcat caagcctttc aaggtaaagc ctcacccgtt ccagggagcc ggggtccttc
      361 gggagccact tcccggaagc caggtccgcg gcggtgatct gggtgtaggg ttccggcagg
      421 ttgccctcag ggtccctcca gaagcccggg tgggatatgt ccaggacgta gtgagtatcc
      481 atcgtcaaat gatagttgcc gatgctcccc ttccggtcca gcctgtcgag cagcgcgcaa
      541 accctttccg tatcacccgg tgccccgggg acaaaaaggg cgcctcccgg tttgcaaaag
      601 tcattctggg catcgatgac cagcacatcg tagagggtca aaggaaccac tcctttcttg
      661 gccatgcacg cggtattcga gagggcggac gaggtcacta ttcatcgggc aatcgatcct
      721 tggcctgacc tatacccccc ctggatgggg gatcttcatt gacgcggttc gaaccgtact
      781 ccccgcaggg gggccgtgtt gaccgttagg gtgaagacat cgggtcggga atagtggcca
      841 tcggaatcca tgagcaagtg accctcggtc accatgccta gatccagcgt ggcgtggacc
      901 gtacccaccg cgtcgtagag gggttccacc aggcaggtgg agtccggtgc gatcacggtg
      961 ctcccccccc tttgaagcag ctccgccggg ttccccatgg aggagagcat ctcgcggcac
     1021 tcctgtatcg cccccgggag agaatcataa ccctcgagca catcgcctac tgtcaggacc
     1081 gtccccgccg cgagcacgaa acactggccc tcgaaggcat aatgcctgct ggccaccagg
     1141 tgcagatccc tcacccaggg ccactgtgcc acgtggacgg tttccttcct ggcgtgcatt
     1201 gccgcccgtg ccagggggag ccagtgttcc cagcagatga ggccccctag gactccgaag
     1261 ggggcgtcga ccgagacgag ggtgcttccg tcgcccattc cccagaggag ccgttccgtg
     1321 taggtgggcg tgagcttcct gtgagccgaa tataccccgt cgggggagat gaagagcatc
     1381 gtgttataga gcgtgttccc gaccagttcg tgggctccca tgaccatatg gactttggac
     1441 tcaacggctg ccctggcaag ggccgcggtc tcatcaccct gtagggtgag ggcattctcc
     1501 acgagccttc ggaaaaggcc tttcgccgcg tcgtgccccc agagggccgc ctcgggggcc
     1561 gagtcgatcc aaacggggta acccgggagc caagtctcgg ggaaaaccac cactcgagcg
     1621 ccatcaccgg cagccttgcg ggtcagcgcg caggcttttt ccacgctggc accgaggttc
     1681 aaaaaaaccg ggggttcctg tattatcgca acgttcactt ctttaacttt ttccgtcatg
     1741 tcctcctcct atcaataccg ccctcctcgc cgccctggcc ctggcaag
//
LOCUS       DAOSRG010000248         1787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_61088_length_1787_cov_11.855658, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000248 DAOSRG010000000
VERSION     DAOSRG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61088_length_1787_cov_11.855658"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..557
                     /locus_tag="PLM30_06685"
     CDS             48..557
                     /locus_tag="PLM30_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOR54894.1"
                     /translation="MTERERRLEKLAQVLTESDDPMSGAELAKLFNVSRQAIVQDIAL
                     LRDRGLRVMATARGYVHREAKPRRIFPVRHGVEEIADELNIMIEAGGRVIDVVVDHPV
                     YGEIRGNLDLASREDVARFVNILRSTGQSPLLSLSRGFHAHTVEADDDMTLDAIEKAL
                     ADKGYLEMN"
     gene            581..778
                     /locus_tag="PLM30_06690"
     CDS             581..778
                     /locus_tag="PLM30_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54895.1"
                     /translation="MTRKEKLWVLFSLLYGIVNVAVPFTILKKAGSFAFWNLITAAVL
                     LAGVWVTSGWAGKTDHEGEAR"
     gene            778..>1787
                     /locus_tag="PLM30_06695"
     CDS             778..>1787
                     /locus_tag="PLM30_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOR54896.1"
                     /translation="MSRTLILAGIALWFTLGSLLSWHARRNLGKGMIEYFLADRKVGG
                     FISAMTYSATTYSAFMMVGLVGLTYSSGIGSLGFEMTYLAATVILMVIFAPRYWAAGR
                     IYRLVTPSELLSKRYGSPSAGAVSAVLCLVMLIPYASVQLMGTGYLLEILSGGSISFT
                     AGMIIAAGVSFVFSWWAGLRSVALTDAFQAVIMLAASLLLLGFIAFALFPEGLPGALS
                     ARRDLLEVSWSFPLFLGLTLPWAFFAVTNPQVVQRLYIPSSVGNLRKMILGFSAFGFV
                     YTVLCVVFGLAAAAKMPGLEKADNAMAMLLSSVPAPLALVVFLSIMAAAVSTMNSIIL
                     TL"
BASE COUNT          305 a          552 c          548 g          382 t
ORIGIN      
        1 tttgctatta tctgccggta gcagccagaa gaagagaggg ctccattttg acagagagag
       61 aaagaagact ggaaaaactt gcccaggtcc tcaccgaaag cgacgatcca atgagcggcg
      121 ccgaactggc caaactcttc aatgtcagcc gccaggccat cgtccaggac atcgcccttc
      181 tgagggaccg cggcctccgg gttatggcca cggcgcgggg ttacgtccac cgggaggcga
      241 aacccaggag gatattcccc gtccgccacg gcgtggaaga gatcgccgac gaactcaaca
      301 tcatgatcga ggccgggggg cgggtgatag acgtggtggt ggaccacccc gtctacggcg
      361 agatccgggg caacctcgac ctggcttcca gggaggatgt ggcccgcttc gtcaacatcc
      421 tgaggagcac gggccagtcg cccttgctat ccctgtcgcg aggttttcat gcccacaccg
      481 tggaggccga cgatgacatg acgctggacg ctattgaaaa agcccttgcg gacaagggtt
      541 acctcgagat gaattgaaca ctttcacggg aggcgaggcc ttgactcgca aggagaagct
      601 gtgggttctt ttttcgctgc tctatgggat cgttaacgtg gccgtgccct tcaccattct
      661 caagaaggcc ggttccttcg ctttctggaa cctcatcacc gcggccgtgc tccttgcggg
      721 ggtatgggtg acttcgggct gggcgggaaa aaccgaccat gaaggagagg cccggtgatg
      781 agccgtacgc tgatcctggc gggcatcgcc ctttggttca ccctcggctc cctcctgtcg
      841 tggcatgcca gaaggaacct cggtaagggc atgatcgagt acttcctggc ggaccgcaag
      901 gtcggcgggt tcatctcggc catgacctac agcgcgacca cctacagcgc cttcatgatg
      961 gtcggcctcg tggggctcac ctactcctcc ggcataggca gcctcggttt tgagatgacc
     1021 tacctggcgg cgacggtgat actgatggtg atcttcgccc ccaggtactg ggcggcgggg
     1081 aggatctacc ggctcgtgac cccgtcggaa cttctttcaa aacgctacgg cagcccatcg
     1141 gcgggggccg tgtcggccgt cctgtgcctg gtcatgctca tcccctacgc ttcggtccaa
     1201 ctgatgggga cgggctacct cctggagatc ctctctgggg gctccatatc ctttacggcg
     1261 ggcatgatca tcgcggcggg cgtgtccttc gtcttctcat ggtgggcggg gttgaggtcc
     1321 gtggccctga ccgacgcttt ccaggccgtg ataatgctcg ccgccagctt gctgctgctg
     1381 ggcttcattg ccttcgccct cttccctgag gggttgcctg gggccctatc ggccaggcgt
     1441 gacctcctgg aggtatcctg gtcctttccc ctgttcctgg ggctgaccct cccctgggcg
     1501 ttcttcgccg tgacaaaccc ccaggtggtg caacgccttt atattccatc gagcgtggga
     1561 aacctcagga agatgatcct gggcttttcc gccttcggtt tcgtctatac cgtcctgtgc
     1621 gtggtcttcg gcctggcggc cgcggcgaag atgccggggc ttgagaaagc cgacaacgcc
     1681 atggccatgc tgctctcatc ggtcccggcc ccactggccc tcgtcgtctt cctcagcatc
     1741 atggctgcgg cggtgtcgac catgaactcc atcatcctga ccctgag
//
LOCUS       DAOSRG010000249         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_61505_length_1779_cov_6.277842, whole genome shotgun sequence.
ACCESSION   DAOSRG010000249 DAOSRG010000000
VERSION     DAOSRG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61505_length_1779_cov_6.277842"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1779)
                     /locus_tag="PLM30_06700"
     CDS             complement(<1..>1779)
                     /locus_tag="PLM30_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOR54897.1"
                     /translation="GPAFAQLAEGGSGGEIAIPDIPGTSEAGSIQAPIIPDLGGSGIP
                     DIFPSQPDSQFSPSPTPGQAETPSQWADADPMAEFERFLTGTLHEEQLTRISRYGSEF
                     FRTAPSTFAPDEAAPVRPDYVIGPGDQLRIDVWGMVEGSWTVTVSRDGTITIPRVGVI
                     GVAGLDFDQLQDVLDEQFSRFYSNYEMSVTLGALKSIKVYVVGNARRPGGYTISSLST
                     LVNALLVSGGPNESGSMRSVQVKRGGRVITDFDLYDLLMKGDKTKDIRLMPEDVIFIP
                     NVGPQVAVTGNVRRPAIYEMKKPVSVNDLIEMAGGMTSTGFEGRIQMMRVVDRQYRTM
                     LEGDLRALSPNSLKATTLQDGDFIRLFSVVERQSIVRITGPVAKPGQFAINQGVNRVS
                     DVLHWAGGLLHYAADEAEITRVDVTDRGPQTTRIKISLGAAMQGDPMHNIPLQMNDYI
                     FVRAVPDWQLYKQVAVYGEVTYPGVYTIGPGERLSSLIERAGGFSLNAYTRGAIFTRE
                     SVKKAQQQQIDDMVQRFEKDLLAVGLNELSSALTPEETRMLQMEAQQKSRLLDALRNT
                     KATGRITTMIAASNVLRGSAYDIELE"
BASE COUNT          266 a          495 c          661 g          357 t
ORIGIN      
        1 tgctccagtt cgatgtcgta ggccgagccc ctgaggacgt tcgaggcggc gatcatggtg
       61 gtgatgcgcc ccgtggcttt cgtgttcctg agggcgtcga ggaggcggct cttctgctgg
      121 gcctccatct ggagcatcct tgtctcctcg ggcgtgaggg ccgaggagag ttcgttgagg
      181 cccacggcaa ggaggtcctt ctcgaagcgc tggaccatgt cgtcgatctg ctgctgctgg
      241 gccttcttta cgctctcgcg ggtgaagatg gcaccgcggg tgtaggcgtt gagggaaaat
      301 ccgccggcgc gctcgatgag ggacgacagg cgctcgccgg ggccgatggt gtagacgccg
      361 gggtaggtta cctcgccgta gacggcgacc tgcttgtaga gctgccagtc gggcacggcc
      421 cggacgaaga tgtagtcgtt catctgcagg ggtatgttgt gcatcgggtc gccctgcatg
      481 gccgccccga ggctgatctt tatcctggtg gtctgggggc cgcgatcggt gacgtcgacg
      541 cgggtgatct cggcctcgtc ggctgcgtag tggaggaggc cgcccgccca gtggagaacg
      601 tcgctgaccc tgttgacgcc ctggttgatg gcgaactgcc ccggcttggc cacggggccg
      661 gtgatgcgga cgatgctctg ccgctccacc acggagaaga ggcggatgaa gtcaccgtcc
      721 tgcagggtgg ttgccttcag ggagttgggg gagagggccc tgaggtcgcc ctcgagcatg
      781 gtgcggtatt gcctgtcgac gacgcgcatc atctggatgc gcccctcgaa gccggtggag
      841 gtcatgcccc cggccatctc gatgaggtcg ttgacgctga cgggtttctt catttcatat
      901 atggcggggc gcctgacgtt gcccgtgacg gccacctggg gtcccacgtt ggggatgaag
      961 atgacgtcct cgggcatgag gcggatgtcc tttgtcttgt cgcccttcat gaggaggtcg
     1021 tagagatcga agtcggtgat gaccctgccg ccgcgcttga cctggacgct cctcatggag
     1081 ccgctctcgt tggggccgcc ggagacaagc agggcgttga ccagggttga gagggaggag
     1141 atggtgtaac ctccgggcct gcgggcgttg cccacgacgt agacctttat gcttttgagg
     1201 gcgccgaggg tgacgctcat ctcgtagttg gagtagaagc gggagaattg ctcgtcgaga
     1261 acgtcctgga gctggtcgaa gtcgaggccc gcaacgccga tgacgccgac gcggggtatg
     1321 gttatggtgc cgtcacggct tacggtgacc gtccaggagc cctcgaccat tccccagacg
     1381 tcgatgcgta gctggtcgcc ggggcctatg acgtaatcgg gccggaccgg agcggcctcg
     1441 tcgggcgcga aggtcgaagg cgccgtgcgg aaaaactccg agccgtagcg gctgatcctg
     1501 gtgagctgtt cctcgtggag ggttcccgtg aggaagcgct cgaactcggc catgggatcg
     1561 gcgtcggccc actgggaggg cgtctctgcc tggcctggtg ttggtgacgg ggagaactgg
     1621 ctgtcgggct gggacgggaa gatgtcggga attccggatc ctccgaggtc ggggatgatc
     1681 ggggcctgga tggagccagc ctcgctggtg cccgggatgt cggggatggc aatctctccg
     1741 ccggaacctc cctctgcaag ttgcgcgaac gctggtccg
//
LOCUS       DAOSRG010000250         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_62015_length_1769_cov_4.579347, whole genome shotgun sequence.
ACCESSION   DAOSRG010000250 DAOSRG010000000
VERSION     DAOSRG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62015_length_1769_cov_4.579347"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLM30_06705"
     CDS             <1..263
                     /locus_tag="PLM30_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR54898.1"
                     /translation="DTCESHARFIGKHNLTVLLLSDPEKKVLELYGAWRIKKMYGKES
                     MGVARSTVLVSPRGEVAKTWKSVRAAGHAARVLEVIREMARA"
     gene            316..1062
                     /locus_tag="PLM30_06710"
     CDS             316..1062
                     /locus_tag="PLM30_06710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOR54899.1"
                     /translation="MAAKVSIDLYSLTLFLAGIASSRRPWYHPGEEAITLDEWTRPDP
                     SRMALLAIDVQREFQPGFPSGRPENASAVPAAAAVARAFRKAGKPVIHVVRLYLPDGS
                     NADLCRRSGIVTGKPLLLPGSESSQIAVELLPHPSVLLDHELLLAGRAQEIGEAEWVI
                     YKPRFGAFYRTPLKEHLRKLGVNTLVITGSNYPNCPRATIYEASERDFRVVAVTDAIS
                     RLDEGGIGELEAIGVLCISSKEVLEGLLPH"
     gene            1126..1341
                     /locus_tag="PLM30_06715"
     CDS             1126..1341
                     /locus_tag="PLM30_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54900.1"
                     /translation="MVFLLLEENMMVHLGRVLALVRFEGETAVLLRDGSVMATGFTPP
                     TLAKRSARFMEEGKELARSLRQGGIDL"
     gene            1338..>1769
                     /locus_tag="PLM30_06720"
     CDS             1338..>1769
                     /locus_tag="PLM30_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR54901.1"
                     /translation="MTPASASASASSQYTAKDIQILEGLEAVRRRPSMYIGDTGPRGL
                     HHIVYEVVDNAIDEAMAGWCSNIVVKVHGDGHVSVEDNGRGIPTDPHPQTGRPAAEVV
                     LTTLHAGGKFDKKSYQVSWGLHGVGVSVVNALTEWLNLTVWR"
BASE COUNT          356 a          553 c          530 g          330 t
ORIGIN      
        1 ccgacacctg cgaaagccac gcccgcttca tcggcaagca caacctgacg gtcctgctcc
       61 tcagtgaccc cgaaaagaag gtccttgaac tctacggggc ctggcggatc aagaagatgt
      121 acggcaagga atccatggga gtggcaagga gcaccgtcct ggtgtcgccc cggggcgagg
      181 tggccaaaac ctggaagagc gtaagggccg ccggtcacgc cgcgagggtg ctggaagtca
      241 tcagggagat ggcccgcgcc tgattcacct ccaagtcaac gaagctctta gccctcgccc
      301 cgcgggggca tttttttggc ggcaaaggtc agtattgatt tatattctct tactcttttc
      361 ctggccggaa tcgcctcttc ccgccgtcca tggtatcatc caggtgagga ggcgatcacc
      421 ctggacgaat ggaccaggcc cgatccatcg aggatggccc tgctcgccat cgacgttcaa
      481 cgggagttcc agcccggttt cccctcggga aggccggaga acgcttcggc cgtcccggcg
      541 gccgccgccg tcgcccgggc cttcagaaag gccggaaagc ccgtcattca tgtcgtccgg
      601 ctctacctcc ccgacgggag caacgccgac ctctgcagga ggtccgggat cgtaacgggg
      661 aaaccactcc tccttcccgg gtcggagtcg agccagatcg ccgtcgaact gctgccccat
      721 ccgtcggtcc tccttgacca cgaactgctc ctcgccggaa gggcgcagga gatcggcgaa
      781 gccgagtggg tcatctacaa gccccgcttc ggagcctttt acaggacccc cctcaaagag
      841 caccttcgaa aattgggagt gaacaccctc gtgatcacgg gatccaacta ccccaactgc
      901 ccccgggcca ccatctacga ggccagcgag agggatttcc gcgtcgtcgc ggtgaccgac
      961 gccatttccc ggctggatga aggcggcatc ggtgaactcg aggccatcgg agtgctctgt
     1021 atctcgtcga aagaggtgct ggaagggctc cttccccact gataacaacg ccttttggta
     1081 tataataata gggttattac aatctccttc caggagggac ccccgttggt gtttcttctc
     1141 ctagaggaaa acatgatggt gcacctgggc agagttcttg cccttgtcag gttcgaaggc
     1201 gagactgccg tgctccttag ggacggttct gtgatggcga cggggttcac cccgccgacc
     1261 cttgccaaga gatcggcccg cttcatggag gaaggaaaag aattggcgcg ttcgcttcgc
     1321 cagggaggaa tagatctatg acaccagctt cagcttcagc ttcggcttcc agccagtaca
     1381 ccgcgaagga catacagatc ctcgaagggc ttgaagcggt tcggcgaagg ccgagcatgt
     1441 atatcggaga taccggcccc aggggccttc accatatcgt ctacgaggtg gtcgacaacg
     1501 ccatcgacga ggcgatggcc gggtggtgtt caaacatcgt cgtcaaggtg catggcgacg
     1561 gccacgtctc cgtggaagac aacggccggg ggatccccac cgaccctcac ccccagacgg
     1621 ggaggcccgc cgccgaggtg gtgctcacca cccttcacgc cggtgggaag ttcgataaaa
     1681 agtcctacca ggtcagctgg ggccttcacg gcgtcggggt gtcggtcgtc aacgctctga
     1741 cggagtggct caacctcacc gtctggcgc
//
LOCUS       DAOSRG010000251         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_62797_length_1754_cov_4.703355, whole genome shotgun sequence.
ACCESSION   DAOSRG010000251 DAOSRG010000000
VERSION     DAOSRG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62797_length_1754_cov_4.703355"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLM30_06725"
     CDS             <1..438
                     /locus_tag="PLM30_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOR54902.1"
                     /translation="KGKVTVVDTPRDAAWGDAIVCVTQSKEKFLKDEWIARGTKVFPM
                     GSYQEVDDDFILSADRVIVDHVGQCLHRGALKELVEAGKFKESDIDGTIGEVVAGKKP
                     GRLSDDDRVLCIPIGTGAMDIAVATVILERAKEKGMGGSFDFV"
     gene            complement(479..1087)
                     /locus_tag="PLM30_06730"
     CDS             complement(479..1087)
                     /locus_tag="PLM30_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR54903.1"
                     /translation="MPEVFERNTTDNENNQIDLHIGRRIRSIRRGAQLTLDVLAQRTG
                     LSKALLSKIENGKVSSPISTYAKIARSLGVSLSELLREAEETRFLLIRKEERKPRSSR
                     KTPYGYSFESLGARWPNKSWNPFLMTYEPLPEPHSSPGFNYEGEEFIYVLEGRLEFFC
                     GDSKYELAPGDCLFVDGGLPHGGRALGDEKCLALLVVLPRNP"
     gene            1185..>1754
                     /locus_tag="PLM30_06735"
     CDS             1185..>1754
                     /locus_tag="PLM30_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOR54904.1"
                     /translation="MKEFLLRKVKVRKVSFGKSTEVKNGCLTIGADALAEVLLEDRQI
                     KSVDIRIAGPGKSIRIVPVKDVIEPRAKLPEQESPFPGVFSDPHVAGEGVTVALDGVC
                     VVTTGKMVNFQEGLIDMSGPGAEFSTFSRKNNVVLVIEPADGLSKHEHERAVRLTGLR
                     AASFLGKAGLSAEWEEEIRVPGTNLPGLYG"
BASE COUNT          369 a          491 c          558 g          336 t
ORIGIN      
        1 aagggcaagg tgaccgtggt cgacaccccc cgagacgccg cctggggcga cgccatcgtc
       61 tgcgtcaccc agtccaagga aaaatttctg aaggacgaat ggatagcccg cgggacgaag
      121 gtcttcccca tgggatccta ccaggaggtg gacgatgatt tcatcctgtc cgccgatcgg
      181 gtgatcgtgg atcacgtcgg tcagtgcctt cacagaggag ccttgaagga gctggtcgag
      241 gccgggaagt tcaaggaatc ggacatcgac ggcaccatcg gagaggtcgt cgcgggcaag
      301 aagccgggcc ggctctccga tgacgaccgg gtcctctgca tacccatcgg gacgggtgcc
      361 atggacatag ccgtcgctac ggtcatactc gagagggcca aggaaaaagg gatgggcgga
      421 agtttcgact tcgtgtaggg cccatgggag aaaggacctc acgatcgggt ccgccgcttt
      481 agggattcct ggggaggacc accaaaaggg cgaggcattt ctcgtcgccc agggcccttc
      541 caccgtgggg gagaccgccg tcgacgaaga ggcagtcccc gggggccagt tcatacttgc
      601 tgtcgccgca gaagaactcc agcctgccct ccaggacgta gataaattcc tcgccctcgt
      661 agttgaaacc cggcgaactg tggggttcgg gcagaggctc gtaggtcatc aggaaggggt
      721 tccagctctt gttcggccac cgggctccca gagattcgaa ggaatagccg tagggcgtct
      781 tccggctgga gcggggtttc ctttcttcct tgcggatgag gaggaaacgg gtctcctcgg
      841 cttccctgag gagttcgctc agggaaacgc ccagggaacg ggctattttg gcataggtgg
      901 atatgggtga gctgaccttg ccgttttcga tcttggaaag gagggcctta gaaaggccgg
      961 tcctctgcgc caggacgtcc agggtcaact gcgcccctcg ccggatgctc ctgatccttc
     1021 ttcctatatg gaggtcgatc tgattgtttt cattgtcggt agtgtttctt tcaaaaacct
     1081 cgggcatggg acgccctcct tcaaagaaag ggtttgttga accgccagaa acctattcta
     1141 gcacctgcgg cgtccggaag gcatagaaaa agggggatta taccgtgaag gaattccttt
     1201 tgagaaaggt gaaggtccgg aaagtgagct ttggaaagtc caccgaggtg aaaaatggtt
     1261 gcttgaccat cggcgccgac gccctcgccg aggtccttct ggaggaccga cagataaaaa
     1321 gtgtcgatat ccggatcgcc ggacccggta aaagtatccg catcgtcccc gtgaaggacg
     1381 tcatcgaacc ccgggcgaaa ctaccggaac aggagagccc ctttcccggc gtcttctctg
     1441 acccccacgt ggcaggcgag ggcgtcaccg tggccctgga cggcgtctgc gtcgtgacga
     1501 cgggcaaaat ggtcaacttc caggaagggc tcatcgatat gtcggggccc ggcgcggaat
     1561 tctccacctt ttcccgcaaa aacaacgtgg ttctcgtcat cgaacccgcc gatggcctca
     1621 gcaagcacga gcacgagagg gcggtcaggc tgaccggcct cagggccgcc tcctttctgg
     1681 gaaaggcggg cctctcggcc gagtgggaag aagaaatccg cgtgccggga acgaacctcc
     1741 ccggccttta tggc
//
LOCUS       DAOSRG010000252         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_62957_length_1751_cov_9.696344, whole genome shotgun sequence.
ACCESSION   DAOSRG010000252 DAOSRG010000000
VERSION     DAOSRG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62957_length_1751_cov_9.696344"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLM30_06740"
     CDS             complement(<1..1043)
                     /locus_tag="PLM30_06740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR54905.1"
                     /translation="MRETPLPSFDLTRNWERVRDETLAAINVVLDSQHFIQGPEVEAL
                     EKDLAAYLGTAHTVGCASGTDALLLALMALDLGPGDEVITTPFSFFATASCVTRLGAT
                     PVFADVMPDSYNIDPKEVLRKISPRTKAVIPVHLFGQMSRVEEFAPRLAEKGISIVED
                     CAQAIGSTRSVDGKTLRAGAVGELGCFSFFPTKNLGCFGDGGLISVSSERLDRRLRSL
                     RAHGSGEQYFHDEVGLNSRLDELQAAILRVRLRHLETWNEERRLVAERYRILFAEQDL
                     GEWVTPPLEEKGNRHIYHQYVIRCRSRDLLKENLAGRGITTRIYYPLSLHLQPCFSYL
                     GYGRGDMPVSENLT"
     gene            complement(1155..>1751)
                     /locus_tag="PLM30_06745"
     CDS             complement(1155..>1751)
                     /locus_tag="PLM30_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54906.1"
                     /translation="RIITKQLKPGQRLPEVNIAVQLAVSRTPVREALRRLASEGLVLI
                     IPNSGARLASPTQREMEDTFAVREQLESISVAIAATRISERNLRRLEDAILEEEKAFQ
                     EKNLELFLEINENFHRIIAESSGNRVLAEFVENILARTNAYVVFYEPFYDMEENPGIA
                     QHRDIAKALREKDREKAVELMREHLEFSLCGLNRPSRQ"
BASE COUNT          320 a          523 c          508 g          400 t
ORIGIN      
        1 gtgaggtttt cagaaacggg catatcgccc ctgccgtagc caagataaga aaagcagggt
       61 tggaggtgga gcgacaaggg gtagtagatg cgggtagtga tccccctccc ggcgaggttt
      121 tctttcaaaa gatccctgga ccggcatctt atgacgtact ggtggtatat atgccgattg
      181 cccttttctt cgagaggggg ggtcacccat tcgccaaggt cttgctcggc gaagagtatc
      241 ctgtatcttt ccgccacaag cctccgttcc tcattccagg tctcgaggtg acggagcctc
      301 acccggagaa tggcggcctg cagttcatcg agcctgctgt tgagcccgac ctcgtcatgg
      361 aagtactgtt cgcccgaacc gtgggccctc aggcttctaa gccgcctgtc cagcctttcc
      421 gacgatactg agatcaggcc gccgtcgccg aagcacccca ggttcttcgt cgggaagaag
      481 gagaagcagc cgagttcgcc cactgccccg gcccttaggg tcttcccatc cacggacctg
      541 gtagacccaa tggcctgggc gcagtcctcg acgatgctga tccccttctc cgcgagccga
      601 ggggcgaatt cctccaccct ggacatctgg ccgaaaaggt gaacggggat cacggccttc
      661 gtcctcggcg agatcttccg tagcacttct tttgggtcga tattgtagga atcgggcatg
      721 acatcggcaa aaacgggggt ggctcccaac ctggtgacac agctagcggt ggcgaagaag
      781 ctgaaaggcg tcgtaatgac ctcgtcaccg gggcccaggt cgagggccat gagggcgaga
      841 aggagcgcgt cggtccccga tgcgcatcct accgtatggg ccgttcccag gtaggccgcg
      901 aggtccttct ccagggcttc cacctcgggg ccctgtataa aatgctggct atcgagtacg
      961 acgttgatgg cggccagggt ttcatccctg acgcgctccc agttcctggt gagatcgaag
     1021 gacggcagcg gcgtctccct catctaacgc tcccctttca agatcctttt cttctccaag
     1081 tacccggtgg cacttttcac acttactatt ttcacgttgt cgggggagcg agtggcagct
     1141 tgcccgggtg cgccctattg gcgggaaggc ctgttcagac cgcaaagaga gaactccagg
     1201 tgttccctca tgagctccac ggccttttcc cggtcctttt ccctgagggc cttggctata
     1261 tccctgtgtt gggcgatacc cgggttctcc tccatgtcat aaaaaggttc gtagaagacc
     1321 acgtaggcgt tggtcctcgc cagaatgttc tcgacgaact cggccaacac cctgttaccg
     1381 ctcgattcgg cgattatgcg gtggaaattt tcgttgatct cgaggaaaag ttcaaggttc
     1441 ttctcctgga aggccttctc ttcctcgagg atcgcgtcct cgagccgcct gaggttcctt
     1501 tcgctgatcc gtgtcgccgc tatcgctacg gagatgctct caagctgctc cctgacggca
     1561 aaggtgtcct ccatctccct ttgcgtaggt gacgcgagcc tggcgccgct gttagggatt
     1621 atgaggacaa ggccctcaga tgccagcctg cgaagggcct ctctcaccgg ggtacggctc
     1681 accgccagct gtacggcgat gttgacctcc ggaagccttt gtccgggctt gagctgcttc
     1741 gtgatgatcc t
//
LOCUS       DAOSRG010000253         1744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_63318_length_1744_cov_12.510361, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000253 DAOSRG010000000
VERSION     DAOSRG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63318_length_1744_cov_12.510361"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PLM30_06750"
     CDS             <1..1023
                     /locus_tag="PLM30_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR54907.1"
                     /translation="KELEELRSLGVRLKLITGDNRYVARHVAASVGIKAARILTGPEL
                     AVMRDEALWHLVGHVDIFAEVDPNQKERIIRALQRMGSVVGFLGDGINDAPALHAADV
                     GISVDKAVDVAKEAADFVLMEHSLAMVRQGIQIGRRTFANTIKYIFITTSANFGNMIS
                     MAAGSLFLPFLPLLAKQVLLNNFMSDIPSVGIAGDRVDPEWEREPHRWNIRIIRDFMV
                     IFGLVSTAFDLLTFYALIRLAGSAASAFRTGWFIESLLTELLVLFVVRTYRPFYRSRP
                     GTFILASSALLGLLAVALPYLPAGRVFGFVPLPWPVLAAVLAITLLYLVVSESTKRAF
                     FRFLRL"
     gene            1077..>1744
                     /locus_tag="PLM30_06755"
     CDS             1077..>1744
                     /locus_tag="PLM30_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOR54908.1"
                     /translation="MPWWKRNKKAIKRGILGALLLLGLVTAVAHLGEIGRFLGILREA
                     KPAWVAAGLSFQAQTYLCAALVWKAAMANIGIHFPLSQLVPLSVAKLFSDQVLPSGGL
                     SGSAFFTVALKHRGVAVLDGLVALVASLTGYYASYMIMAVASLALLAFRHDIHPWILT
                     ASVLFFVVALAVPAVVILASLYGGERIPEAFKRRFPIPVMEDALGKVSARVIFDPLVL
                     AQVT"
BASE COUNT          269 a          625 c          521 g          329 t
ORIGIN      
        1 aaggaactgg aggaactccg ctccctcggg gtccgcctca agctgatcac gggggacaac
       61 cgctacgtgg cccgccatgt cgccgcctcg gtggggatca aggccgcccg gatcctcacc
      121 ggcccggaac tggcggtcat gagggacgag gctctgtggc acctcgtggg ccacgtcgac
      181 atcttcgccg aggtcgatcc caaccagaag gaacggatca tccgggcgct gcagaggatg
      241 ggttccgtcg tgggcttcct gggcgacggc atcaacgacg ccccggcgct ccacgccgcc
      301 gacgtgggca tctcggtgga caaggccgtc gacgtcgcca aggaggcggc cgacttcgtc
      361 ctgatggaac acagcctggc catggtccgc caggggatcc agatcggccg ccggaccttc
      421 gccaacacga taaaatacat cttcatcacc accagcgcca acttcgggaa catgatcagc
      481 atggcggcgg ggtcgctctt cctccccttc cttcccctcc tggccaagca ggtgctcctc
      541 aacaacttca tgtcggacat cccctccgtg gggatcgccg gcgacagagt ggaccccgag
      601 tgggagaggg aaccccaccg gtggaacatc aggataatca gggacttcat ggtcatcttc
      661 gggctcgtga gcaccgcctt cgacctgctg accttctacg ccctcatcag gctggcgggc
      721 agcgcggcaa gcgcgttccg gaccgggtgg ttcatcgaat ccctgctgac cgaactgctg
      781 gtgctgttcg tggtgaggac ctacaggccc ttctaccgta gccgacccgg caccttcatc
      841 ctcgcttcct ccgccttgct ggggctcctc gccgtggcgc ttccctacct cccggcgggc
      901 cgtgtcttcg gcttcgtccc cctcccttgg cccgtcctgg cggccgtcct ggccattacc
      961 ctcctctacc tggtcgtctc ggaatcgacc aagagggcct ttttccgctt cctgcgatta
     1021 tgacgtcgcc gcccgccccc caagaccggg tacccgtaac ggaggcgaat gatacggtgc
     1081 cctggtggaa aaggaacaaa aaggccatta agcggggcat cctgggcgcc ctgctcctgc
     1141 tggggctcgt caccgccgta gcccacctcg gcgagatagg ccgcttcctg gggatcctcc
     1201 gggaggcgaa acccgcctgg gtggccgccg gcctgtcctt ccaggcccag acttacctct
     1261 gcgcggccct ggtctggaag gcggccatgg cgaacatcgg catccacttt cccctttccc
     1321 aactggtccc gctgagcgtg gccaagctat tctccgacca ggtcctcccc agcggaggcc
     1381 tcagcggttc cgcctttttc accgtggccc tcaagcaccg gggggtagcg gtcctggacg
     1441 gcctcgtggc cctggtggcc agcctcaccg gttactacgc ctcctacatg atcatggcgg
     1501 tggcgagcct cgccttgctg gctttccgcc acgacatcca cccctggatc ctcacggcgt
     1561 cggtgctgtt cttcgtcgtc gccctcgccg tccccgccgt ggtgatcctg gccagccttt
     1621 acggggggga aaggataccg gaagccttca agcgccgctt ccccatcccc gtcatggagg
     1681 acgccctggg gaaggtctcc gcccgggtca tattcgaccc cctggtgctg gcccaggtga
     1741 cctt
//
LOCUS       DAOSRG010000254         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_63682_length_1737_cov_8.121879, whole genome shotgun sequence.
ACCESSION   DAOSRG010000254 DAOSRG010000000
VERSION     DAOSRG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63682_length_1737_cov_8.121879"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLM30_06760"
     CDS             <1..675
                     /locus_tag="PLM30_06760"
                     /inference="COORDINATES: protein motif:HMM:NF039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOR54909.1"
                     /translation="SLPEGTRAFLSLARKLGYLAAHLSREPATQLKVSLRGPLFSPAE
                     EPVCFEIPYHFSPFTVAGLKGYLEYSHGPEVNYMSAPMLAEDKGIRIEESRTGPGTWR
                     NQVELELLSPGFREGISVSGTITEEGRQRVVNIGGYWIEFIPEGTVLLFSNHDRPGVI
                     GKVGTLLGRAGTNIANFALGRRNGSGLAVGALQIDDDVPMPVLEELKKDVDLLWAVKV
                     RFTEDL"
     gene            676..1311
                     /locus_tag="PLM30_06765"
     CDS             676..1311
                     /locus_tag="PLM30_06765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOR54910.1"
                     /translation="MKRIFLVRHGETDWNREGRFQGRRDTPLNDRGRGQARSAAGALL
                     RSSVDRVVSSPLSRAAGTAEATASLLGLGLETHPGLIEIDHGLWEGHTAGEVESLWPG
                     MLTLWHSRPEEVTMPGGENLHEVSRRAWGAFREILAGPGDALALFSHDAVLKVLLCRV
                     FDAPLSSFWRFRLGNGSITLLEETPRGLEVLVLGEACHLGSAWERVVQEGL"
     gene            complement(1319..>1737)
                     /locus_tag="PLM30_06770"
     CDS             complement(1319..>1737)
                     /locus_tag="PLM30_06770"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOR54911.1"
                     /translation="DPSRVGQAKRVVGFDPFVIDNPHINGNRLLRILRDNPGIRCYLL
                     NTGRVGGKDGANITVEATTTAVREAMRETLEWRYDDILGYEIPSSLPVPQGGDLDPYR
                     WYSREEYASMIGDLRRERREYLQQFKGLAPEIVDGV"
BASE COUNT          278 a          562 c          599 g          298 t
ORIGIN      
        1 tccctccccg agggcaccag ggccttcctc tcgctggccc gcaagctcgg ctacctggcg
       61 gcgcacctat cgcgggagcc ggcgacacaa ctgaaggtca gcctgcgggg ccctctcttt
      121 tcgccagccg aagagccggt atgcttcgag atcccttacc atttctcccc cttcaccgtg
      181 gcggggctca agggctacct ggagtactcc cacggccccg aggtgaacta catgtcggca
      241 ccgatgctgg cggaggacaa gggcatcagg atcgaggaga gccgtaccgg ccccgggacc
      301 tggcgcaacc aggtcgagct ggaactcctc tcgcccggtt tccgggaggg catctccgtg
      361 tcggggacga tcaccgaaga ggggcgccag agggtggtca atataggagg ctactggata
      421 gagttcatcc ccgagggaac ggtgctcctc ttcagcaacc acgaccgccc cggcgtgatc
      481 gggaaggtgg ggaccctcct ggggagggcc gggacgaaca tagccaactt cgccctgggg
      541 cgcaggaacg ggagcgggct ggccgtgggg gccctgcaga tcgacgacga cgttcccatg
      601 cccgtcctgg aggagctgaa aaaggatgtg gacctgctct gggcggtgaa ggtccgcttc
      661 acggaggatc tctagatgaa gcggatcttc ctggtcaggc acggcgagac ggactggaac
      721 cgggaagggc gcttccaggg caggagggac acccccttga acgaccgggg ccgggggcag
      781 gcccgctccg ccgctggggc gctcctgcgc tcttccgtgg acagggtggt gtcgagcccc
      841 ctttcccgcg ccgccgggac ggcggaggcc acggcctccc tccttgggtt aggtcttgag
      901 acccacccag gcttgatcga gatagaccat ggtctctggg aggggcacac cgccggggaa
      961 gtggaaagcc tgtggccggg gatgctcact ctctggcact ccaggcctga ggaggtgacc
     1021 atgccggggg gggagaacct ccatgaggtc tcccgtcggg cctggggggc cttcagggag
     1081 atcctggcgg gcccgggaga cgccctggcc ctcttctccc acgacgcggt cctcaaggtc
     1141 ctcctctgcc gggtctttga cgcgccactg agcagcttct ggaggttccg cctcgggaac
     1201 ggcagcatca ccctcctcga agagacgccc cgggggctgg aggtcctcgt ccttggcgag
     1261 gcctgccacc tcgggagcgc ctgggagagg gtcgtccagg aagggctcta gcgcccggtc
     1321 agaccccgtc gacgatctcc ggggccagtc ccttgaactg ctgcaggtac tcccttcttt
     1381 ccctgcgaag gtcgccgatc atggaggcgt actcctcccg ggagtaccac cggtaggggt
     1441 cgaggtcacc accctggggc accggcagcg acgagggtat ctcgtaaccc aggatatcgt
     1501 cgtagcgcca ctccagggtc tctctcatgg cttccctgac ggccgtggtg gtggcctcga
     1561 cggtgatgtt ggcaccgtcc ttgcccccga ccctcccggt gttgaggagg tagcaccgga
     1621 tgcccgggtt gtcccgcagg atcctcagca gcctgttgcc gttgatgtgg gggttgtcaa
     1681 tgacgaaggg atcgaagccc accacccttt tggcctggcc gaccctggag gggtcgt
//
LOCUS       DAOSRG010000255         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_63981_length_1731_cov_8.193317, whole genome shotgun sequence.
ACCESSION   DAOSRG010000255 DAOSRG010000000
VERSION     DAOSRG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63981_length_1731_cov_8.193317"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLM30_06775"
     CDS             complement(<1..535)
                     /locus_tag="PLM30_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOR54912.1"
                     /translation="MEGFKTPVELAKSACLAAKAKVAWSIPQMLLLGVMAGAYIAFGG
                     WLMTVVTHDLAAHMGLGFSKFLGGAIFSVGLMLVVIAGAELFTGNCMMPLGVMAGCIP
                     MSGVVRNWFWVYVANLLGSVIVALLIFYSGLWKGPIGINAFKIAAGKMSLPIGEAVFR
                     GILCNWLVVLAVWMSMAA"
     gene            983..>1731
                     /locus_tag="PLM30_06780"
     CDS             983..>1731
                     /locus_tag="PLM30_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOR54913.1"
                     /translation="MDHRSVGLSDSEYSAIVEALGRQPNQAELRILGVMWSEHCSYKS
                     TRRLLAGLPTKGDFVVLGPGENAGVVDIGGGWGIAFKLESHNHPSAVAPYDGAATGVG
                     GIVRDVLAMGARPVALLNGLCLGRQDSPQAAETADGIVEGIAGYGNTLGVPTIGGKTI
                     YDQCFDFNPLVNAMCLGLVPLGGIVSSRTAKPGHMAVLIGSRTGKEGVAGAAFASSEL
                     IGTKAPNLPRVPKGDPLLEKRLIEGCLEIHS"
BASE COUNT          389 a          498 c          514 g          330 t
ORIGIN      
        1 tggccgccat gctcatccac acggcaagca ctacgagcca gttacagagt atgcctcgga
       61 aaacggcctc gcctatgggg aggctcatct tgccggccgc gatcttgaag gcgttgatgc
      121 cgatcggtcc cttccagagg ccggaataaa agatcagtaa cgctacaata acactcccga
      181 gcaagttggc cacgtagacc cagaaccagt tcctgacgac tccgctcatg gggatgcagc
      241 ccgccatgac gccaaggggc atcatgcagt tccccgtgaa aagttccgcc ccggcaatga
      301 ccactagcat caggccgacg ctgaatatcg cgccgccgag aaacttcgag aaacccaggc
      361 ccatgtgagc tgcaaggtcg tgggtcacga cggtcatcag ccatcccccg aaagcaatgt
      421 aggccccggc catgaccccc aacagaagca tctggggaat gctccaggcc accttggcct
      481 ttgctgcaag acatgctgac ttggccaatt caacaggagt cttgaaacct tccattaaag
      541 cgattcctcc tcatgaatat tagtcccccc gagggggccg atgataaaac cctcattcct
      601 gtggggatga tcagagaccc ccagcggcac gcctggtatt ctaacatgga atgttatttt
      661 ttacactaat agaccctact ttccactcat cctttcacat gactctcgaa aatattgaca
      721 tttatgctcg aagtgcgaat aatgtaagta gattgagggt cattttttat caaagaatgg
      781 tgctcttagg atcaaggccc ccaggttacg aggttgtcgg cgaggggccg ccttgatggt
      841 agagcttccg cccggcggag atggtcaccg aggcaggtag gatcaaaggg acggaccgtc
      901 actggggtga ttgcctccaa tgcggcgacc ccttgttgag atgggttgac agccgggatc
      961 aagggaggtt gcgaaaatgg aaatggacca ccgatcggta ggactcagcg actctgaata
     1021 cagcgcgatc gtcgaagccc tggggcggca accgaaccag gcggaactca ggatccttgg
     1081 tgtcatgtgg tccgagcact gcagttacaa gtccacgaga aggctcctgg ccggcttgcc
     1141 caccaagggg gacttcgtgg tgctgggacc cggggagaac gccggcgtcg tcgatatcgg
     1201 cggaggttgg gggatagctt tcaagctgga gagccacaac cacccgtccg cggtggctcc
     1261 ctacgacggc gccgccaccg gtgtgggcgg tatcgtcagg gacgtcttgg ccatgggggc
     1321 caggccggtg gccctgttga acggactctg cctggggagg caggattcac cccaggccgc
     1381 ggagaccgcc gatgggatcg tggagggcat cgcagggtac gggaacaccc tcggcgtccc
     1441 caccataggt ggcaagacca tttacgatca gtgcttcgac tttaaccccc tggtcaacgc
     1501 gatgtgcctc gggctggtgc cgctgggcgg catcgtcagt tccaggacgg cgaaaccggg
     1561 gcacatggcc gttctgatag gttcaaggac cgggaaagaa ggggtcgctg gagccgcctt
     1621 cgcttcttcc gagttgatcg gcaccaaggc cccgaacctt ccccgggtcc caaaagggga
     1681 tcccctcctg gaaaaaagac tgatcgaagg ctgcctcgag atccattcga a
//
LOCUS       DAOSRG010000256         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_64075_length_1729_cov_8.116487, whole genome shotgun sequence.
ACCESSION   DAOSRG010000256 DAOSRG010000000
VERSION     DAOSRG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64075_length_1729_cov_8.116487"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLM30_06785"
     CDS             <1..657
                     /locus_tag="PLM30_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOR54914.1"
                     /translation="GIAPFAMVVGVAAREAGLDAAQGTFFSLSMIAGTAQLAAVQLYG
                     AGASAAIVLLTAMVVNLRYSMYSLSLHPVLEKRSYIERLLAAFLLSDQSFVVSMTELE
                     TNPDNPSLPTFLFGSSLAVFVVWVAGIFLGFVLKAIIPAGLSLDFVIPLAFMFLLVPH
                     LGGKDRQISVLASIVASVVLVPRLPLQSGLLVSILIGIGSGMAAGEFLRRAGSGGERP
                     "
     gene            654..971
                     /locus_tag="PLM30_06790"
     CDS             654..971
                     /locus_tag="PLM30_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOR54915.1"
                     /translation="MRFYLLFGVLAVALLVIKGLFLCFVPNRKIPPFLERSFRYIPPA
                     VLAALIAPSVFYAGNPAGLDVSPARLAAGLAAFAVALRTKNILITIATGMILLWVFSF
                     LSA"
     gene            1116..1571
                     /locus_tag="PLM30_06795"
     CDS             1116..1571
                     /locus_tag="PLM30_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR54916.1"
                     /translation="MKKVVVAVDGSDVSKEVIDYALHYAQREKDAELLFLHVVNWEDY
                     REVSVGGRSVSVPPSEEEAGKELGDWVQERVGSSGTPKPERMSINVLFGNPYNEIVSF
                     AEKMDASMIMIGHRGLSNLERFFLGSVAAKVVAHSPCSVYVHREKSIPG"
BASE COUNT          322 a          491 c          513 g          403 t
ORIGIN      
        1 ggcatagcgc ccttcgccat ggtggtagga gtggctgcaa gggaggcggg gctggatgcc
       61 gcccagggaa cattcttctc cctctctatg attgccggta cagcccagct ggcggcggtc
      121 cagctttatg gcgccggggc ttcggccgcc atcgtgcttc ttaccgccat ggtcgtgaac
      181 ctcagatact ctatgtacag cctttcactg caccctgtac ttgaaaagag gtcatacatt
      241 gaacgcttac tggcggcttt cttgttgtcg gaccagtctt tcgtggtctc catgaccgaa
      301 ctggagacca accccgataa cccgtcgctc cctacattcc tgttcggttc atcgctggcg
      361 gttttcgtgg tctgggttgc ggggatcttc ctgggctttg tcctgaaagc cattatcccg
      421 gcggggcttt cccttgattt cgtcataccc ttggccttca tgttcttgct cgtaccccac
      481 ctgggcggca aggacagaca gatctccgtc ctggcgtcca tagtggcctc ggtggtgctg
      541 gtcccgcggc ttcccctcca gtcgggcctt ttggtgtcga tcctcatagg cattgggtcg
      601 ggaatggccg ccggtgagtt tttaaggaga gccggaagcg ggggggaacg cccatgaggt
      661 tctacctcct tttcggcgtt ctcgccgtag ctctgctggt catcaaggga ctgttcctgt
      721 gcttcgtgcc taatcggaag atccctcctt tcctcgagag gtctttccgc tatatcccac
      781 cggccgtcct tgcagccctc atcgcgcctt cggtcttcta cgccgggaac cccgcggggc
      841 ttgacgtttc accggcgagg ctggcggcgg ggctggcggc cttcgccgtc gcgcttagga
      901 ccaagaatat tttgatcacc attgcaaccg gcatgatcct gctctgggtc ttttccttcc
      961 tttcggccta ggatcgaaca ggttcatcct cgggttgccc tggaaccgcc ttcaagaatg
     1021 ccggataaag cggtagaata aaaacgtggt ccggcgtcac tccgatgccg gccggagaaa
     1081 tggagcgagt ccagcgggag gaaggggtga tccctatgaa gaaagtagtg gtagcggtcg
     1141 acggtagcga tgtcagcaag gaggtcatcg attacgccct ccactatgct cagagagaaa
     1201 aggacgccga gctgctgttc cttcacgtgg tcaactggga agactaccgc gaggtatccg
     1261 tcggcggccg ttccgtgtcg gtgcctccct cggaagagga ggccggaaaa gaattgggtg
     1321 attgggtcca ggaaagggtg ggatcatcgg gtacacccaa gcccgaaagg atgtccatca
     1381 atgtactctt cggcaatccc tataacgaga tcgtatcctt cgcggaaaag atggacgcct
     1441 ccatgataat gatcgggcac cgggggctga gcaacctgga gaggtttttc ctgggaagcg
     1501 tggcggcgaa ggtcgtcgcc cattccccct gtagtgtcta cgtccacagg gaaaagagca
     1561 tccccgggta acaaccgtaa aagtcggaag gaggaggtgc catgatcgat ctgaaggact
     1621 acctcgctcc gggttattcc catgagtata agttgactgt gaccagcgag gaagcggtcg
     1681 gcaatttttc acccaaactc gaccagcttc tggccacctc ggcctgcgt
//
LOCUS       DAOSRG010000257         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_64802_length_1716_cov_7.926550, whole genome shotgun sequence.
ACCESSION   DAOSRG010000257 DAOSRG010000000
VERSION     DAOSRG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64802_length_1716_cov_7.926550"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..676)
                     /locus_tag="PLM30_06800"
     CDS             complement(134..676)
                     /locus_tag="PLM30_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOR54917.1"
                     /translation="MQRFYGVEVVIVEREPILDRVESLGIEYERKYGGCSQCVIGAIR
                     DTFGGIDDSVFKAATGLAGGVGLTGATCGALTGAAMAIGAFLGREYDQFADPERIRFT
                     TFELVRRMVARFEEEYGSTLCQHIQEKIMGRSYRLYDEGEHKAFIEAGGHDDKCPVVV
                     GKAARWAAEILFDEGLVQGH"
     gene            complement(690..935)
                     /locus_tag="PLM30_06805"
     CDS             complement(690..935)
                     /locus_tag="PLM30_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54918.1"
                     /translation="MTQRGAIEDVVAVSVNMTVGVARERASIIVVTRESNQGYIKKLG
                     LTNAPDFSTATGLAEKKSGDIESIGIITHGGDFAPKF"
     gene            complement(1038..>1716)
                     /locus_tag="PLM30_06810"
     CDS             complement(1038..>1716)
                     /locus_tag="PLM30_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HOR54919.1"
                     /translation="HMDELTVVDTFGGLNPHSVPYLIKKVKEHIPDKPIGVHFHDDFG
                     LGAATTIMGLASGADIAHTSISGIGERAGNAPYEDVAMALLTMYGVDTGLRYEKIYPL
                     SKYLREITGLTVRQNRGIVGDYVADIESGIVAAWYKNVEGIAPLELSPFLYDLVGHPD
                     TKVVIGKLSGLPTVDIYLDKLGIETDDKDAKAEILKRIKDTALEKRRLLTIEEFGEIA
                     TSVLDKS"
BASE COUNT          342 a          539 c          443 g          392 t
ORIGIN      
        1 gcccctgccc cgagacgtag atcctgccgc ccaccaccag gccctgggaa tagggtccta
       61 tcgggtcggg cgaccgatcg gtgcggatct ctcttttcat tgagttgacc tcccaggttg
      121 agatcttaaa tgctcaatga ccttgcacaa ggccctcatc gaacaggatc tcggccgccc
      181 atcgggcggc cttaccgacg acaacaggac atttgtcatc gtggcccccg gcttcaataa
      241 aggccttgtg ctcaccttcg tcatagagcc tgtaggagcg gcccatgatc ttttcctgaa
      301 tatgctggca cagggtgctt ccgtattctt cttcaaagcg ggccaccatg cgacgaacca
      361 gttcaaaggt cgtgaacctg atcctttcgg gatcggcgaa ctgatcgtac tcccttccca
      421 ggaaggcacc gatcgccatt gctgcaccag tcaaggcccc gcaggtagcc cctgtcagcc
      481 caactccgcc ggccagacca gtagcagcct tgaacacgct gtcgtcaatc ccgccgaaag
      541 tatcccggat ggctcctata acgcactggg agcatccccc gtacttcctt tcatactcaa
      601 tacccaggga ttcgaccctg tcaagtatcg gttcacgttc cacgatgacc acctcaaccc
      661 catagaatct ttgcataatt tcacttttgt taaaacttgg gtgcgaaatc tccaccgtgg
      721 gtgatgatcc ctatgctttc gatgtctccc gatttctttt ctgccaatcc cgttgccgta
      781 gagaaatcgg gggcattggt cagacccaac tttttaatgt atccctggtt gctctctctt
      841 gttactacga taatagaggc cctttccctg gcgaccccga cggtcatgtt gaccgacact
      901 gccacaacat cttcgatagc gcctcgttgg gtcatttaaa aaactgcatc cggaggcaac
      961 gcccccaaac ctacaaaatc cgggtgccca gggcaaacac cctcaggaga cggggtcagg
     1021 agaatgacag aacctcctta ggacttgtcc aggaccgacg tcgctatctc tccgaactcc
     1081 tcgatggtca gcaggcgcct cttctccagc gccgtgtcct tgatcctctt caggatctcc
     1141 gccttggcgt ccttgtcgtc ggtctcgatg cccagcttgt ccaggtagat atccaccgtg
     1201 ggaagaccgc taagcttccc gatgaccacc ttcgtgtcgg ggtggcccac caggtcgtag
     1261 aggaaggggg aaagttcaag gggcgctata ccctccacgt tcttgtacca ggccgctacg
     1321 atccccgact cgatatcggc cacgtaatcc cctacgatgc ccctgttctg ccgcaccgta
     1381 agccccgtga tctcccggag gtatttcgag agggggtaga tcttctcgta gcggagaccc
     1441 gtgtcgacgc cgtacatcgt cagaagggcc atggccacat cttcgtaggg cgcgttgccg
     1501 gccctctcgc cgatgcccga tatggaggta tgggctatgt ccgctcccga ggcgagcccc
     1561 atgatcgtcg tggccgctcc caggccgaag tcgtcgtgga aatggacacc gatgggcttg
     1621 tcgggtatgt gctccttgac cttcttgatg aggtagggga ccgaatgggg gttcagcccg
     1681 ccgaaggtgt ccaccacggt gagttcgtcc atgtga
//
LOCUS       DAOSRG010000258         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_65299_length_1707_cov_9.552058, whole genome shotgun sequence.
ACCESSION   DAOSRG010000258 DAOSRG010000000
VERSION     DAOSRG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65299_length_1707_cov_9.552058"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLM30_06815"
     CDS             <1..611
                     /locus_tag="PLM30_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOR54920.1"
                     /translation="GAKAGLFTPDKTALDWVTPRAARSFEPAFPERGAPYEKEIVVEV
                     SDMTPLVALPHSPANVRPALEVGPMPVDQVFIGSCTNGRLTDLEQAARVLRGREVHPS
                     VRCVVIPASREIFADALRLGLIDTFIQAGAVVCTPTCGPCIGGHMGILAKGERCVSTS
                     NRNFVGRMGDAGSEVVLSGPLVAAASAVAGRVADPSEVLDND"
     gene            604..1116
                     /locus_tag="PLM30_06820"
     CDS             604..1116
                     /locus_tag="PLM30_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOR54921.1"
                     /translation="MTRTITGKAWVFGDDVDTDVIIPARYLVTSDPKILGKHCMEGLV
                     PDFSTRVAPGDFIVAGENFGCGSSREHAPLALKGAGCGGVIAASYARIFFRNAINVGL
                     PIFECPEASKAIAEGDTLEVDTSSGTITMTGSGEIFHFSPFPPFIEEIVAAGGLVPFV
                     REELERRKTR"
     gene            1113..>1707
                     /locus_tag="PLM30_06825"
     CDS             1113..>1707
                     /locus_tag="PLM30_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOR54922.1"
                     /translation="MTLRSLRIATIAGDGVGPEVTAEAKKAVDTAGGAFGFKSEWLDF
                     PFGGLHYLETGEVLPDEAIQEIARCDALLLGAIGRPEVKPGILERGILLALRFHFDQY
                     INLRPSRSLPGVGTPIPFRDFDIVVVRENTEDFYMGIGGRSDGSPLESSFPIKRGLYS
                     GEGSLYLSFDRPMACAAQVGLATEPAVRRSTAWACDKA"
BASE COUNT          314 a          525 c          553 g          315 t
ORIGIN      
        1 ctggggccaa ggcggggctc ttcactcccg ataaaacagc cctggactgg gtgacgcctc
       61 gggcggcgag gagcttcgaa ccagcctttc ccgaaagggg agccccctac gagaaggaaa
      121 tagtggtgga agtatcggat atgacccccc tggtggcgct cccccactct ccggccaacg
      181 tccgccccgc cctcgaggtg ggccccatgc cggtggacca ggtgttcatc gggtcctgca
      241 ccaacggccg cctcaccgat ctcgagcagg ccgcccgggt cctgcggggg agggaggtcc
      301 acccctcggt gcgctgcgtc gtgatccccg cttccaggga gatcttcgcc gacgctctcc
      361 ggttgggttt aatcgacacc tttatccagg ccggagccgt ggtctgcacg cctacttgcg
      421 gcccctgcat aggcgggcac atggggatcc tggcgaaggg cgagcggtgc gtgtcgacca
      481 gcaacaggaa cttcgtcggc cgcatgggag atgccggcag cgaggtcgtg ctttcgggac
      541 ccctcgtcgc ggcagctagc gccgtggccg gccgtgtggc ggatccctcg gaggtgttag
      601 acaatgacta ggacgatcac tggaaaggcc tgggttttcg gggatgacgt ggacacggac
      661 gtgataatac ccgcccgcta ccttgtcaca tcggacccga aaatactcgg gaaacactgt
      721 atggagggac tggtccccga cttctccaca agagtggcac cgggcgactt catagtcgcc
      781 ggcgaaaatt tcggctgcgg ctcgtcgcgg gaacacgccc ctttggccct gaaaggtgcc
      841 ggatgcggcg gtgtcatcgc cgcttcctac gctaggatct ttttccgcaa cgccatcaac
      901 gtggggcttc ccatttttga gtgccccgaa gcgtcaaagg ccatagcgga gggcgacacc
      961 cttgaagtgg acacctcttc gggtaccatc accatgaccg gcagcgggga gatctttcat
     1021 ttctccccct tcccgccttt catcgaagag atagtggccg cgggagggct cgtccccttc
     1081 gtgagggagg aactggaaag gaggaagact cgatgaccct gcgatcattg aggatcgcca
     1141 ccatcgccgg ggacggggtg ggccccgaag tcacggcgga ggctaaaaaa gccgtcgata
     1201 ccgccggtgg ggccttcggc ttcaagtcgg agtggctcga ctttcccttc ggggggcttc
     1261 attaccttga aacaggagag gtattacccg atgaagccat acaggagata gcccggtgcg
     1321 acgcgctgct cctgggagcc ataggcaggc cggaagtgaa gcccggtatc cttgaaaggg
     1381 gcatcctgct ggccctgcgc ttccatttcg accaatatat caatctcagg ccctcccggt
     1441 ccctccccgg tgtgggtacg cccataccgt tcagggactt cgacatcgtg gtggtgcggg
     1501 agaataccga ggatttctac atgggcatcg ggggcagaag tgacggctcg ccgctggaat
     1561 cgtccttccc catcaagagg ggcctctaca gcggagaggg gagtctatac ctatcctttg
     1621 acaggcccat ggcctgcgcc gcccaggtgg gcctggccac ggaaccggcg gtgcgaaggt
     1681 cgacggcgtg ggcctgcgac aaggccc
//
LOCUS       DAOSRG010000259         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_65522_length_1703_cov_5.461772, whole genome shotgun sequence.
ACCESSION   DAOSRG010000259 DAOSRG010000000
VERSION     DAOSRG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65522_length_1703_cov_5.461772"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PLM30_06830"
     CDS             complement(<1..1324)
                     /locus_tag="PLM30_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOR54923.1"
                     /translation="MWQYFTERSKKVIQLAHREALRMGHDLIGTEHILMGLVAEGDGV
                     AARILQSSGITLEGLRDAVEQFVGRGDQKSKPVDLPLSPRAKKVLDLAMREAKEMSVN
                     YVGTEHILLGLISEGEGVAAQVLNAAGLDVEKIRGEVIAAVSAGEGSPAESAPDAEEE
                     GPQKPSGRSKTPTLDQLGIALTEMAHRNELDPVIGRAKEIQRLIQILSRRTKNNPVLI
                     GDPGVGKTAIVEGLAQKIIAGDIPEVLKGKRVVQLNMGNLVAGTKYRGEFEERMRKLV
                     KELRDCRDVILFIDEVHTIVGAGGAEGAVDAANILKPSLSRGEFQVIGATTIDEYRKN
                     IEKDAALERRFQPVMVEEPGVDDSIRILEGLRDRYEAHHRAKITDRALEAAARLSARY
                     ITERHLPDKAIDLIDEAAARARLKTMEAPETVKEMERRLESLRKEKEAA"
     gene            complement(1368..>1703)
                     /locus_tag="PLM30_06835"
     CDS             complement(1368..>1703)
                     /locus_tag="PLM30_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOR54924.1"
                     /translation="GLVKQEQAARGKILSGKGIDLEDKVWRAWALLGFSRVMEAREAL
                     ELLSLVRMGSGMGILPSVRVGTMNALLLDTQKAHIQARATGDLSLEDLAAKRSAILRK
                     ALASGEKGS"
BASE COUNT          276 a          581 c          471 g          375 t
ORIGIN      
        1 cggccgcctc cttctccttg cggagggact caagccgccg ttccatctcc ttgaccgttt
       61 ccggggcctc catagtcttg agcctcgccc gggccgccgc ctcatcgatc aggtcgatgg
      121 ccttgtcggg gaggtgacgt tcggtaatat aacgggccga gagcctggct gcggcctcaa
      181 gggccctgtc ggtgatcttc gccctgtggt gggcctcgta acggtccctc agcccttcga
      241 ggatccttat cgagtcatcc acgccgggct cttccaccat gaccggctga aaccgtcttt
      301 ccagggcggc gtccttctcg atattcttcc ggtattcgtc gatagtggta gcgcctatca
      361 cctggaattc acccctggag aggctaggct tgaggatgtt ggcggcgtcg acggcccctt
      421 ccgcaccccc ggcacccaca atggtgtgga cctcgtcgat gaagaggata acatctcgac
      481 aatcgcggag ctccttgact agcttgcgca tcctctcctc gaactcgccc cggtacttgg
      541 tgccggcgac gaggttaccc atgttgagct gcaccaccct tttccccttc aaaacctccg
      601 ggatgtcgcc ggcgatgatc ttctgggcta ggccctccac gatcgccgtt ttgccgacgc
      661 cggggtcgcc tatcagcacc gggttgttct tggtccgccg cgagaggatc tggatgagcc
      721 tctggatctc cttggccctg ccgatgacgg gatccagttc gttgcgatgg gccatctccg
      781 tcaacgctat ccccagctgg tccagtgtcg gcgtctttga gcgccccgat ggcttctggg
      841 ggccctcctc ctccgcatcg ggggcggatt cggcgggaga accctctcca gccgaaacgg
      901 cggcgatcac ctcgcccctg atcttctcga cgtccaggcc cgccgcgttg aggacctggg
      961 cggccacgcc ctctccctcg gagatgagcc ccaaaaggat gtgctcggtc cccacgtaat
     1021 tgacggacat ctcctttgct tcgcgcatgg ccaggtcgag gacctttttc gccctggggc
     1081 tcaagggcag gtccacaggc ttgctcttct ggtcgcccct cccgacgaac tgctcaacgg
     1141 catcccgtag cccttcgagg gttatgcccg aggactggag gatccgggcg gccacaccat
     1201 ccccctccgc aacgagtccc atgaggatgt gttccgtccc gatcaggtca tgacccatcc
     1261 tgagagcttc ccgatgggcc agttgtatca ctttcttgct tctttcagtg aaatattgcc
     1321 acattaacta aacctccatt aaaaggaagg gtgagcaggc cctgcgttca acttcccttt
     1381 tcaccgctgg cgagcgcctt cctgagtatg gccgaacgtt tcgccgccag gtcctccagg
     1441 gataggtccc cggtcgccct ggcctgtata tgagccttct gggtatccag gagaagggcg
     1501 ttcatcgtgc caaccctgac agaggggaga atacccatcc cggatcccat cctgaccagg
     1561 gaaagcaatt ccagggcttc cctggcctcc attaccctgg aaaaacctag cagggcccag
     1621 gccctccaca ccttgtcctc gaggtcgatc cccttcccgg acaggatctt gccccgggcg
     1681 gcctgttcct gcttcaccag ccc
//
LOCUS       DAOSRG010000260         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_65801_length_1698_cov_8.452222, whole genome shotgun sequence.
ACCESSION   DAOSRG010000260 DAOSRG010000000
VERSION     DAOSRG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65801_length_1698_cov_8.452222"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLM30_06840"
     CDS             <1..530
                     /locus_tag="PLM30_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR54925.1"
                     /translation="IRDDSPAAVEGMKALGLLPVMVTGDNEVTARAVARQVGITEVLS
                     GVRPEDKLDIVRTYQAKGKKVLMIGDGINDAAALKGADIGMAIGSGMDLAIDSADIVV
                     MSGGITKALESIKISERTFKVITRNLFWAFLYNIIAIPVAMAGLLHPAIAETAMAFSS
                     ISVVLNSLTIRKGEI"
     gene            531..734
                     /locus_tag="PLM30_06845"
     CDS             531..734
                     /locus_tag="PLM30_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOR54926.1"
                     /translation="MGREYSYKVPDMSCGHCVKRITGVLESMGMSDFEVSLEEKKIFS
                     GSPLEEKVVQALEEAGYPVTPEN"
     gene            830..>1698
                     /locus_tag="PLM30_06850"
     CDS             830..>1698
                     /locus_tag="PLM30_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR54927.1"
                     /translation="MFRFPSKGNSDLSLVLCGAAGQGVQTLEEILVDSFRKAGYHVFA
                     SREYMSRVRGGSNSTEIRVSSKRVRALVDRMDILLPLNGGIRANIRERVTPETLILGD
                     AAEIKDELADRGGTFLNVPLMEKGREAGGPVYTGIVAAGLLAGLLGVPPEIPEGLLKE
                     RFIAKSEQVVKNNLSAFEKGMGLFRELVASKGVSVDIAKENEAVGEMMMNGSDAVSLG
                     ALAGGCDFMSSYPMSPATGVLTYLARHARSHAVAVEQAEDEIAAINMALGASYGGARS
                     MVSTSGGGLSLMG"
BASE COUNT          366 a          451 c          579 g          302 t
ORIGIN      
        1 agataaggga cgactccccc gccgccgtgg aggggatgaa agccctgggg ctcctgccgg
       61 tcatggtgac cggcgacaac gaggtgaccg ccagggccgt ggcccgccaa gtgggcatca
      121 ccgaggtcct ttcgggtgtg aggcccgagg acaagctcga tatcgtgagg acctaccagg
      181 ccaagggcaa gaaggttctc atgataggcg acggcatcaa cgacgcggcg gccctcaagg
      241 gggccgatat cggaatggcc atcggttccg ggatggacct ggccatcgac agcgccgaca
      301 tcgtggtcat gagcggcggc attacaaaag ccctggagag catcaagata tcggagcgga
      361 ccttcaaggt catcacaagg aaccttttct gggcttttct gtacaatatc attgccatac
      421 cggtggccat ggccgggctc ctacaccccg ccatcgccga gaccgccatg gccttcagct
      481 cgatctcggt ggtgctgaat tcgctgacca taagaaaggg ggagatctga gtgggcaggg
      541 agtattccta caaggtaccg gacatgtcat gcggtcattg tgtgaagagg ataaccgggg
      601 tcctggagtc gatggggatg agcgacttcg aggtttccct cgaggagaag aagatattct
      661 cgggatcacc cctggaagag aaggtcgtcc aggccctgga agaggccggt taccccgtaa
      721 cgcctgagaa ctgaagtgaa ggcccacccg gcggtgggcc ttcactatca gtgtttaggt
      781 gaatgaacag gacgggcttt gtcccctcct ggaataggga ggagataaaa tgttccgatt
      841 cccctcgaaa ggaaacagcg acctgtcgtt ggttttgtgc ggggcggccg gccagggcgt
      901 ccagaccctc gaggagatcc tggtcgattc gttccggaag gccggttacc atgtgttcgc
      961 cagcagggag tacatgtcca gggtaagggg agggagcaac tccacggaga taagagtttc
     1021 ctccaagaga gtgagagctc tcgtcgacag gatggacatc ctgctcccgc tgaacggcgg
     1081 catacgggcg aacatcaggg aacgggtcac accggagacg ctcatactgg gggatgccgc
     1141 ggagatcaag gacgaactgg ccgacagggg cggcactttt ttgaacgtgc cgctgatgga
     1201 aaagggccgt gaagcgggag gccccgtcta caccgggatc gtggcagcgg gactcctggc
     1261 gggacttctg ggggtaccgc ccgaaatacc ggaaggactg ctgaaggaaa ggtttatcgc
     1321 caagtccgaa caggtcgtga agaacaacct ttcagctttt gagaagggca tggggctttt
     1381 cagggaactg gtggcttcga agggggtttc cgtcgacatc gccaaggaaa acgaggccgt
     1441 cggggagatg atgatgaacg ggtccgacgc ggtctccctg ggggcactgg ccggaggctg
     1501 tgatttcatg tcctcctacc cgatgtcccc ggccacggga gtgctgacct acctggcgag
     1561 gcacgcccgg agccatgccg tcgccgtgga gcaggcggag gacgagatag ccgccataaa
     1621 catggccctg ggcgcctcct atggcggggc caggagcatg gtgagcacct ccggcggcgg
     1681 cctttcgctg atggggga
//
LOCUS       DAOSRG010000261         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_66130_length_1692_cov_10.674404, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000261 DAOSRG010000000
VERSION     DAOSRG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66130_length_1692_cov_10.674404"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /gene="glgP"
                     /locus_tag="PLM30_06855"
     CDS             complement(<1..1474)
                     /gene="glgP"
                     /locus_tag="PLM30_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOR54928.1"
                     /translation="MPKMNYGNNIGVSLRFMMEQDPSFRTVAYFSMEVGLKAGIPTYS
                     GGLGVLAGDILKSAADLGVPMAGVTLLYRKGYFRQTFDDCLQEALPVEWDPGKELTLL
                     PNEVTVIIEERIVRVRAWCLEIQGRTGFTVPVYFLDTDLDGNTPEDRQLTWYLYGGDE
                     RYRLCQEIILGSGGLRMLRDLGYSNIDDYHLNEGHAAFLLLELIREMGYENYDRVRER
                     GIFTTHTPVSAGHDRFSWDLVNRVMHRSMAGRLRRMMPTEDVSMTEIALRYSHYINGV
                     SEKHAEVSRAMYGREDVDCITNGIHSLTWVSPEMAGLFTKYIPGWDNAPERLVKAAQI
                     PVEEIRLAHGAAKKRLLDYVKAATGKDLDPGRLTIGFARRVAHYKRADLVLRDTGRLV
                     KAAGGRVQFIFSGKAHPQDGPAREVLKKLLCEAQNMVGTDIPVVFIEDYDMDKAALLV
                     QGVDLWLNNPIRPREASGTSGMKCALNGIPNFSVLDGWWIE"
BASE COUNT          261 a          564 c          527 g          340 t
ORIGIN      
        1 cctcgatcca ccacccgtcc aggacggaaa agttggggat gccgttgagg gcgcacttca
       61 tcccgctcgt cccggaggcc tcccggggcc tgatggggtt gttgagccac aggtcgacgc
      121 cctgcacgag aagggcggcc ttgtccatat cgtagtcctc gatgaagacg acggggatgt
      181 cggtgcccac catgttctgg gcctcgcaga gcagtttctt cagcacctcc cgggccgggc
      241 cgtcctgggg gtgagccttg ccggagaaga tgaactgcac cctcccgccg gcggccttga
      301 ccagccggcc ggtgtcgcgg aggacgaggt cggccctctt gtaatgggcc accctccggg
      361 cgaacccgat ggtgagcctg ccggggtcga ggtctttccc cgtcgccgcc ttcacgtaat
      421 ccagcagtct cttcttggcc gccccgtggg caaggcggat ctcctcgacg gggatctgcg
      481 cggccttgac cagccgctcg ggggcgttgt cccacccggg gatgtatttc gtgaagagcc
      541 cggccatttc ggggctcacc cacgtgagcg aatggatgcc gttggtgatg cagtccacgt
      601 cctccctccc gtacatggcc ctgctgacct cggcgtgttt ttccgagacg ccgtttatgt
      661 aatggctgta acgcagggct atctccgtca tggagacgtc ctcggtgggc atcatcctcc
      721 gtaaccggcc ggccatggag cggtgcatca cccggttcac caggtcccag ctgaagcggt
      781 cgtggcccgc tgaaaccggc gtgtgggtcg tgaaaatgcc cctctcccgg accctgtcgt
      841 agttctcgta ccccatctcc cggatgagtt ccagcagcag gaaggcggcg tggccttcgt
      901 tgagatggta gtcgtcgatg ttggagtaac ccaggtcgcg gagcatcctc agtccgcctg
      961 agccgaggat gatctcctgg cacagtcggt agcgctcgtc ccccccgtag aggtaccatg
     1021 tgagttgacg gtcctccggg gtgttgccgt cgagatcggt gtccaggaag tagaccggga
     1081 cggtgaaccc cgtcctcccc tgtatttcca ggcaccaggc gcggaccctc acgatccgct
     1141 cctcgatgat caccgtgacc tcgttgggca ggagggtcag ttccttcccg gggtcccact
     1201 cgacgggcag ggcctcctgg aggcagtcgt cgaaggtctg ccggaagtag cccttgcggt
     1261 agagcagcgt gacgcccgcc atgggcaccc ccaggtcggc ggcgctttta aggatatccc
     1321 cggccaggac acccagcccg cccgagtagg tgggtatgcc cgccttcagc ccgacctcca
     1381 tggaaaagta cgccaccgtc cggaacgacg gatcctgctc catcatgaag cgcagtgaaa
     1441 ccccgatgtt gttgccgtag ttcatcttcg gcatccttcg aataccccct gctctctcac
     1501 ttctaggtgc gttcattatc tcaccccggg cgagttttgc ctccccggtg atatgcggcc
     1561 gtgtcgggcc gacatcgccg ccaattcgcc tgccggggcg ttcagcaatt gttcctccct
     1621 caccctccag agccagttgc ccctgggttt cccgggggtg ttgagccgcg cctcggcgcc
     1681 cagcccgagg ag
//
LOCUS       DAOSRG010000262         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_66373_length_1688_cov_10.681568, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000262 DAOSRG010000000
VERSION     DAOSRG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66373_length_1688_cov_10.681568"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PLM30_06860"
     CDS             <1..1425
                     /locus_tag="PLM30_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR54929.1"
                     /translation="AHTHLDFVCASVNDTASKVTQGVTTEIAGNCGLSLIPVRPETIE
                     DLKQYLAPFIPRDVNLSWKWRSLGDWFDSVDERGNSTNVASLVGHGTLRIYAMGFADR
                     KPSGQEMAEMKRALAQTMEEGSLGLSTGLIYPPGCYSDTDELCELCEVVAEHGGLYVT
                     HMRNEGPELLQSVEEALEIGRRSGCPVHISHHKSSGRQNFGLVSRSLRMMEDARAEGI
                     DVTCDVYPYTAGSTLISSLLPKWVHEGGVGRMLERLKDPASRRKIADELDRVIPGWEN
                     LAKGCGWENILICSCEKEPSYEGKTVTEIADGRGTSPEEALFDVILETEGKATIALFM
                     MDEKDCSTVLAHPLSMVGSDGFASSFSGVLSKGKPHPRSFGTFPRVLGKYVREEGLMS
                     LEKAIWKMTGFAAQRFGLKDRGFLREGMVADLVVFDPLEIIDNATYKEPRQQAEGIDY
                     VILGGEVVVDHKVYNGKTLGRSLR"
BASE COUNT          346 a          491 c          536 g          315 t
ORIGIN      
        1 gcccacaccc atctcgattt cgtctgcgct tcggtcaacg acacggcgag caaggtcacc
       61 cagggggtta ccacggagat cgcgggcaac tgcgggctct cgctcatccc ggtgaggccg
      121 gagaccatcg aggatctgaa gcagtacctc gcccccttca tcccccggga cgtgaacctg
      181 tcctggaaat ggaggtccct gggggactgg ttcgacagcg tcgatgaaag gggcaactcg
      241 accaacgtgg cctccctggt cggacacggg accctacgga tatacgccat gggtttcgcc
      301 gacaggaagc ccagcgggca agaaatggcc gagatgaaga gggccctggc ccagaccatg
      361 gaagagggct ccctggggct ttcgacgggg ctcatctacc cgcccgggtg ttactcagat
      421 acggatgaac tctgtgaact ttgcgaggtc gtagcggaac acggcggtct ttacgtgacc
      481 cacatgcgca acgagggccc cgaactgctg cagtccgtcg aggaggccct ggagatcggg
      541 agaagaagcg gctgcccggt gcatatttcc catcacaagt catcggggag gcagaatttc
      601 gggctggtgt cccggtcgct gcggatgatg gaggacgccc gggcggaggg gattgacgtc
      661 acttgcgatg tctaccccta cacggcgggg tccactctca taagcagcct gctccccaag
      721 tgggtccacg agggcggcgt cgggcgcatg cttgagaggc tgaaggaccc cgcgtccagg
      781 cggaagatcg ccgacgaact ggaccgggtc atcccgggct gggaaaacct ggcgaagggc
      841 tgcggctggg agaacatcct catctgctcc tgcgagaagg aaccgtccta cgaggggaaa
      901 accgtcacgg agatcgccga cgggagagga acctcaccgg aggaggcact cttcgacgtg
      961 atcctggaga ccgagggcaa ggccaccatc gcgctcttca tgatggatga gaaggattgc
     1021 tcgacggtcc tcgcccaccc gctctccatg gtgggctccg acgggttcgc cagttctttc
     1081 agcggggtcc tttccaaggg caagccccac ccccgttcct ttggaacctt cccccgcgtc
     1141 ctgggcaagt acgtcaggga agagggtctc atgtccctgg aaaaagccat ctggaaaatg
     1201 acgggcttcg cggcccagcg gttcggcctt aaggaccggg gttttctgag ggaagggatg
     1261 gtcgccgacc tggtcgtttt cgaccccctc gagatcatcg acaacgcgac ctacaaggag
     1321 ccccgtcaac aggccgaggg tatcgattac gtgatcctgg gcggcgaggt cgtcgtggat
     1381 cacaaggtgt ataacgggaa aacccttggg agatccctga ggtgacccgg taccgctttt
     1441 tccgccgact tcccttgcct gtcgcatgag ttggattaga gctattgaca agggaacgac
     1501 gctggtatag agtgcgctga aggcaacaca tttcataatg catattttct aaaggaggga
     1561 acccggtggg tgtagagaac ggctccttcg gccgacttat aaacattatt attctccttt
     1621 ccgccttggg gttcccctcc tggcgcggtt gagccgtact cttccgcaac gaaccgggat
     1681 aaagggac
//
LOCUS       DAOSRG010000263         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_66439_length_1687_cov_7.934436, whole genome shotgun sequence.
ACCESSION   DAOSRG010000263 DAOSRG010000000
VERSION     DAOSRG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66439_length_1687_cov_7.934436"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1687
                     /locus_tag="PLM30_06865"
     CDS             112..>1687
                     /locus_tag="PLM30_06865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR54930.1"
                     /translation="MEQTATLETMVPEDRKGFEGFGLREELLEAVEAKGFTEPMPVQV
                     AVLGREDLDVDLIVRARTGSGKTLAFLLPLMNDPDLDPSRPGVLIISPTRELALQISR
                     EAAWLSRRIECACATLVGGMDMAAQVRELRHGAAFVIGTPGRVLDHVRRGTLKMDHVR
                     TVVLDEGDHMLDMGFREELESILEAAPARERTWLFSATMPPAVRALSRRYLTDPEMIS
                     LVEEGAQHEDIVHRVYTTPRGRRVEGLVNALLWENSSKGLVFCHTRAETIEVADRLAR
                     ERFSSCCLHGDMSQRERNRALAMFASGRVSHLVATDVAARGLDIPDVEHVFQFGLPSD
                     AETFVHRSGRTGRAGNEGQDILVLTLAEAHKLKGLLRDTKISLSWSPVPDAQSIRAMQ
                     RARYEELVLGDSLGGDDGTREWAVSLLEKADPVDLVAAFLDLAAKDIRSGYSLKDDFA
                     REREQAAVRSPRAVPARRERKPLGRKKGTLVRLPVRDAADWNAGKVLRTVCSALDVES
                     REIAEIVLKKDGVMVEL"
BASE COUNT          304 a          525 c          589 g          269 t
ORIGIN      
        1 cgccgatggc gataattgtc ccattgccgt gaagggatta cacgaagtgg cgcccccatg
       61 ggcgccgctt tttttgtgtc cctttatcga atacaggagg aacgtgcttt tatggaacaa
      121 actgcgacac tggaaacaat ggtaccggaa gaccggaagg gttttgaggg tttcggcctc
      181 cgggaagagc tcctcgaggc cgtcgaggcg aagggtttca ccgaaccgat gccggtccag
      241 gtggccgtgc tcggccggga agacctcgac gtcgacctga tcgtccgggc ccggacgggc
      301 tcgggaaaga ccctcgcctt tttgcttccc ctcatgaatg acccggacct cgatccatcg
      361 aggccgggag tgctgatcat ctccccgacg agggaactgg ccctccagat ctcccgcgag
      421 gcggcgtggc tctccaggcg gatagaatgc gcctgtgcca ccctggtggg cgggatggac
      481 atggcggccc aggtccgcga gctgaggcac ggggcggcct tcgtcatcgg gaccccgggc
      541 cgggtgctgg accacgtgcg ccgggggacg ctcaagatgg atcacgtccg caccgtggtc
      601 ctcgacgagg gcgaccacat gctcgacatg ggcttcaggg aggaattgga gtccatcctc
      661 gaggcggccc cggcgaggga gaggacctgg cttttctcgg ccacgatgcc cccggctgtc
      721 agggcccttt ccaggaggta cctcaccgac cccgagatga tatccctcgt ggaggagggc
      781 gcccagcacg aggacatagt ccaccgggtc tacacgacgc cgaggggccg cagggtggag
      841 ggcctggtga acgcgctgct ctgggagaac tcgtcgaagg ggctcgtgtt ctgccatacc
      901 agggcggaga ccatcgaggt ggccgacagg ctcgccaggg agcggttcag ttcctgctgc
      961 ctccacggcg atatgagcca gagggagagg aaccgagccc tggcgatgtt cgcctcgggc
     1021 cgggtttccc acctggtagc caccgacgta gccgccaggg ggctggacat acccgacgtg
     1081 gagcacgtct tccagttcgg cctcccctcc gacgcagaga ccttcgtgca ccgcagcgga
     1141 aggaccggta gggccggcaa cgagggccag gatatcctgg tgctcaccct ggccgaggcc
     1201 cacaaactga agggcctctt gagggacacg aagatctccc tgagctggtc gcccgtcccg
     1261 gacgcccagt ccatcagggc tatgcagagg gcccgttacg aggagttggt cctgggcgac
     1321 tccctcgggg gcgacgacgg cacgagggaa tgggccgtga gcctgctcga gaaggccgac
     1381 ccggtggacc tcgtggcggc cttcctggac ctggcggcca aggacatccg ctccggctac
     1441 tccctcaagg acgacttcgc ccgggagagg gagcaggcgg cggtccgaag tccccgcgcc
     1501 gtcccggccc ggagggagcg caagcccctg gggcgcaaaa aggggaccct cgtacggctc
     1561 cccgtgaggg atgccgccga ttggaacgcc gggaaggtcc tcaggacggt atgctcggcc
     1621 ctggatgtgg aaagccgtga gatcgccgag atagtgctca aaaaggacgg cgtcatggtg
     1681 gaactgc
//
LOCUS       DAOSRG010000264         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_66975_length_1677_cov_7.639334, whole genome shotgun sequence.
ACCESSION   DAOSRG010000264 DAOSRG010000000
VERSION     DAOSRG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66975_length_1677_cov_7.639334"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1677
                     /locus_tag="PLM30_06870"
     CDS             111..>1677
                     /locus_tag="PLM30_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOR54931.1"
                     /translation="MQGKIPGGEDAEWVAAGKLRSGDRIKINDHRSLSEWKGLGTLEQ
                     GYAVGLLAGGDEAVLSLRAPYAKAAGEENMGQGPMAVHSYGSLASSLGMLPGNKGVTR
                     VMETHTSSDFVRGFLRGFFDADGSIRGEQEKGVSVRLSQSSLPTLQAVQRMLLRLGIA
                     GKICKNQERTGQGGMKAHRASARQELVISGENAGLFSERVGFKDPVKQGKLKGGLGFY
                     GRRMEEEPFTATVDRLVPDGIDEVFDVTVPGENSFDANGLVVHNCGEQPLLPYEACNL
                     GSINLGHMVEDGKVDWEKIRLTTRLAVRFLDDVIDVNRYPLPRIEEMVKLTRKIGLGV
                     MGWADMLFQLRIPYDSEEALDLGRSIMGAINAAGRQESAALALSRGACPALPDSGLRN
                     STITTIAPTGSLSMIADCSSGIEPVFALEFTKEVLDGKQFSYRNRYYVEALEGGGSPV
                     TVARVFKTSHQIAPEWHVRMQSAFQEYVDNAVSKTINMPNSATIEDVEKAYVLAWKTH
                     CKGITVFRDGCRSG"
BASE COUNT          347 a          490 c          545 g          295 t
ORIGIN      
        1 tttcttctac cgaagaggga tttttcaaaa ccggggaaaa accggtcttc cgcctggaga
       61 ccagagaggg cttcagcctg aggctcaccg aagaccaccc ggtactgagg gtgcaaggaa
      121 aaatccccgg tggggaagac gccgaatggg tcgccgccgg gaaactcaga tccggcgaca
      181 ggataaaaat taacgaccac cgttctttgt cggaatggaa aggtcttggg acccttgaac
      241 agggttatgc cgtcgggctc ctggccggcg gggacgaggc ggtcctttca cttcgggctc
      301 cctatgcgaa ggccgccggg gaggagaata tggggcaagg ccccatggcc gttcattcat
      361 acgggtccct ggcctcatcc cttgggatgt tgcccgggaa caagggcgtt acgcgagtaa
      421 tggagaccca cacctcttcc gatttcgtaa gaggtttcct ccgtgggttc tttgatgccg
      481 acggttcgat caggggcgaa caggaaaagg gtgtgagcgt ccggctgagc caaagttcat
      541 tgccgaccct ccaggctgtt cagaggatgc tcttgaggct ggggatcgcc ggcaagatct
      601 gcaaaaacca ggaacggacc ggccaggggg gcatgaaagc ccaccgggcg tccgcgcggc
      661 aagaactggt gatctccggg gaaaacgccg ggcttttttc tgaaagggtg ggtttcaagg
      721 accccgtcaa acaggggaaa ctcaagggcg gcctcggctt ttacgggcga aggatggagg
      781 aggaaccttt caccgcgacc gtcgaccggc tggtccccga cggcattgat gaagtgttcg
      841 acgtcacggt cccgggcgaa aactcctttg acgccaacgg gctggtggtc cacaactgcg
      901 gcgagcagcc cctcctcccc tacgaggcgt gcaacctggg gagcataaac ctgggtcaca
      961 tggtggaaga cggcaaggtg gactgggaga agatcaggct gaccaccagg ctggccgtgc
     1021 gcttcctcga cgacgtgatc gacgtgaacc gctaccccct cccccggatc gaggagatgg
     1081 tcaagttgac ccgcaagata ggcctcgggg tgatgggctg ggcggacatg ctcttccagt
     1141 tgaggatacc ctatgactcg gaggaggccc tggacctggg caggagcatc atgggggcca
     1201 taaacgccgc cggccggcag gagagcgccg ccctggccct gtccaggggg gcgtgccccg
     1261 ccctgcccga ttccggtttg cggaactcca ccatcaccac cattgcaccc acggggtccc
     1321 tctccatgat cgccgactgt tcctcgggga tagagcccgt cttcgccctg gagttcacca
     1381 aggaggtcct ggatggcaag cagttctcct atcgcaaccg ttactacgtg gaggccctgg
     1441 aggggggcgg atcgcctgtg acggtcgcca gggttttcaa gacgtcccac cagatagccc
     1501 ccgagtggca cgtcaggatg cagtcggcct tccaggagta tgtggacaac gccgtgagca
     1561 agaccataaa catgcccaac agcgccacca tcgaggacgt ggagaaggcc tacgtcctgg
     1621 cgtggaagac ccactgcaag ggcatcaccg tcttccgcga cgggtgccgt tcgggcc
//
LOCUS       DAOSRG010000265         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_68981_length_1646_cov_12.827781, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000265 DAOSRG010000000
VERSION     DAOSRG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68981_length_1646_cov_12.827781"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLM30_06875"
     CDS             <1..444
                     /locus_tag="PLM30_06875"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOR54932.1"
                     /translation="LPKDHPRVELYGTLDECQAALGLARASCCHDEIHEVIFRLEEDL
                     GAVMGYLALFPGLPEPEPGALDEIVEKVRAVTGETSRFVRPGDSLPGAALHLARTIAR
                     RAERVAVGLHRQGDIGDAPYAWLNRLSDAVYALSLWTDWANRGGR"
     gene            541..1218
                     /locus_tag="PLM30_06880"
     CDS             541..1218
                     /locus_tag="PLM30_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR54933.1"
                     /translation="MFNDPLSPARNIDLRQIVFEKIKEAIVGGFIRPGERLSEVELAD
                     KLAVSRTPVREAIRQLAQTGLVTLEPRRGAFVSLPTVKDASDLYDLRTHLEIIAVRLV
                     TRLQPIEELERWRPVFSVMTDETETDHYLAEDRLFHASLYELSGNRYLTMMLHNIADL
                     INLCRHYSVEGIPLSTFAHEHVAIIDAIETGDPDEAEVVLRGHLERSRDGLLNYLREH
                     PELSASG"
     gene            1231..>1646
                     /locus_tag="PLM30_06885"
     CDS             1231..>1646
                     /locus_tag="PLM30_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR54934.1"
                     /translation="MQSPHLFGYFLALMAAVSWAVAPVLYRRGVDRISYSGLGALRCN
                     GYITSAALFLFLTMGPTAFAPLPAVTMAKIFVCSVLWLVLGDFFYFSSLHKLGVSISV
                     PITSSYPLMVVPAAWIFLGEPAKPMVLVAAVLIVAGL"
BASE COUNT          268 a          618 c          462 g          298 t
ORIGIN      
        1 ctccccaagg accaccccag ggtggagctt tacgggaccc tggacgaatg ccaggccgct
       61 ctcggcctgg ccagggcctc ctgctgccac gatgaaatcc acgaggtgat cttccgcctc
      121 gaggaggacc tgggcgccgt catgggctac cttgcccttt tcccgggcct ccccgagccc
      181 gaaccgggcg ccctcgacga aatagtggaa aaggtccggg ccgttacggg cgagacctcc
      241 cgcttcgtcc gccccggcga ctccctcccc ggcgcggccc tccacctggc caggacgatc
      301 gcccgcaggg ccgagcgggt cgccgtcggc cttcaccggc agggcgatat cggcgacgct
      361 ccctacgcct ggctgaatcg cctctccgac gccgtctacg ctctctccct ttggaccgac
      421 tgggcaaacc gcgggggccg gtagatccaa gggccatcca gacgttgcgc cgtcggccat
      481 gagatgtata atggacagag tatacgtaaa acctccaacc ccttaacggg aggcgttccc
      541 atgttcaacg acccgctctc accggctcgc aacatcgacc ttcgccagat cgtctttgaa
      601 aagataaagg aagccatcgt cgggggcttc atcaggcccg gtgagaggct ctccgaggtc
      661 gagctcgccg acaagctcgc cgtctcccgc acccccgtca gggaggccat caggcagctc
      721 gcccagaccg gcctggtgac gctggaaccg cggcggggag ccttcgtctc ccttcccacc
      781 gtcaaggacg cctccgacct ctacgacctc cgcacccacc tggagatcat cgccgtcagg
      841 ctggtgaccc ggctccagcc catcgaggag ctggagcgct ggcgccccgt cttttcggtc
      901 atgaccgacg agaccgagac ggaccactac ctcgccgagg accggctgtt ccacgcctcc
      961 ctctacgagc tgtcgggcaa ccgctacctg accatgatgc tccacaacat cgccgacctc
     1021 atcaacctct gccggcacta ctctgtggag ggtatcccct tgagcacctt cgcccacgaa
     1081 cacgtggcca tcatcgacgc catcgagacg ggcgacccgg acgaggccga ggtggtactg
     1141 aggggtcacc tcgagcgttc acgggacggg ctcctgaact acctcaggga gcacccggaa
     1201 ctctcggcct caggctagga cctcccccca atgcagtccc cgcatctctt cggctatttc
     1261 ctggccctga tggccgccgt cagctgggcg gtagcgcccg tgctctaccg ccgcggggtg
     1321 gaccgtatat cctactccgg cctcggtgcc ctgcggtgca acggctacat caccagcgcc
     1381 gcccttttcc tcttcctcac catgggaccc acggccttcg ccccccttcc ggcggtgacc
     1441 atggcgaaga ttttcgtctg ctccgtcctc tggctcgtcc tgggggactt tttctacttc
     1501 tcgtccctcc acaaacttgg ggtatccata tcggtgccca tcacgtcctc ctaccccctg
     1561 atggtggtgc ccgccgcctg gatcttcctg ggggaacccg ccaagcccat ggtcctcgta
     1621 gcggccgtac tcatcgtggc cggcct
//
LOCUS       DAOSRG010000266         1642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_69245_length_1642_cov_3.182105, whole genome shotgun sequence.
ACCESSION   DAOSRG010000266 DAOSRG010000000
VERSION     DAOSRG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69245_length_1642_cov_3.182105"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..906
                     /locus_tag="PLM30_06890"
     CDS             64..906
                     /locus_tag="PLM30_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR54935.1"
                     /translation="MSIAVEDLVHVYHPGSPLQTKALDGVTFQAEKGKWMAIVGHTGS
                     GKSTLAQHLNGLLLPTEGSVSVDGIPVRHGEESLRRVRQKVGLVFQYPEQQLFEETVF
                     KEVSFGPRNWGFEASVVKKSVEEALEMVGIGKDLYEASPFRLSGGQKRRVAIASTLSS
                     DPDYLVMDEPTAGLDSSGRRQLLCLLSDLKELGKGIIHITHDMELALGLADTILVLDE
                     GRSALWGTPDVILGELLERDIRGLVIPDLVRFAGRLRDLGFEVPLTWEPGILAEAIGR
                     SHGR"
     gene            903..>1642
                     /locus_tag="PLM30_06895"
     CDS             903..>1642
                     /locus_tag="PLM30_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOR54936.1"
                     /translation="MKFLNNMTLGQYVPVESPVHHLDPRCKIVAVLFSLVGIFLVRGP
                     LGFTMWGLFFLALVATSKIPVGLVLSTVKPVWILVAFTAVIHLFFTGGEPIFSWGILE
                     ITREGVVMASRMGLRLMLLIMFAGMLTLTTSPTELADGLESLFSPFRRFGFPAHELAM
                     MMTIALRFIPTLLNETERIMKAQIARGAELEKGGPLRRLRAFIPVLVPLFVIVFQRAD
                     DLATAMEARNYTGGEGRTRMNPLKWTTR"
BASE COUNT          317 a          491 c          502 g          332 t
ORIGIN      
        1 cctcgatgag gggctctttg ggacggacac cccgccccgg atggaagaga tggtggaggc
       61 cctatgtcga tagccgtcga ggacctggtc catgtctatc accccggttc gccccttcag
      121 actaaagctc tggacggcgt caccttccag gctgaaaagg gcaaatggat ggccattgtg
      181 gggcatacgg ggagtggcaa gtccaccctt gcccagcacc tgaatggcct cctcctgccg
      241 acggagggct ccgtgagcgt cgacggcata cccgtccgcc atggcgagga gtccctccgt
      301 cgggtcaggc agaaggtagg cctcgttttc cagtacccgg agcagcaact cttcgaggag
      361 acggtcttca aggaagtttc cttcggaccc aggaactggg gcttcgaggc ctctgtcgtg
      421 aagaaaagcg ttgaagaggc cctcgaaatg gtaggcatcg gcaaggacct ttacgaagcc
      481 agcccctttc ggctctcggg agggcaaaaa agaagggtcg ccatagcctc tacgctatct
      541 tccgatccgg actacctggt catggatgag cccaccgccg gactcgattc ctcgggaagg
      601 aggcaattgc tctgcctgct ttccgacctg aaagaactgg gcaaggggat aatacacatc
      661 acccacgaca tggaactggc cctgggcctt gcggacacga tactggttct cgatgagggc
      721 aggagcgccc tatggggtac ccccgacgtg atactggggg aactcctcga gcgcgacata
      781 cgggggctgg tcatcccgga cctcgtccgt ttcgcgggcc gtctccgcga cctgggtttc
      841 gaggtgcccc tcacctggga accaggcatc ctcgcggaag ccataggcag gagccacgga
      901 agatgaaatt cctgaacaat atgaccctcg gtcagtatgt ccctgtcgaa tcgccggtcc
      961 accacctcga tccgcggtgc aagatcgtcg ccgtcctttt cagtctcgtg ggcatcttcc
     1021 tggtccgagg cccgctgggc ttcacgatgt ggggcctttt tttcctggcc cttgtcgcca
     1081 cgtcgaagat ccccgtcggg ctggtccttt ccacggtgaa accggtctgg atactggtgg
     1141 ccttcacggc cgtgatacac cttttcttca ccgggggcga gccgatcttc tcctggggca
     1201 tattggagat aacccgggag ggcgtggtca tggcctcgag aatgggtttg aggctgatgc
     1261 tgctcatcat gttcgcgggg atgctgaccc tcacgaccag cccgacggaa cttgccgacg
     1321 gcctggaaag ccttttctcg ccctttcgtc ggttcgggtt ccccgcccat gaactggcca
     1381 tgatgatgac gatagccctg aggttcatac ccaccctttt gaacgagacg gagaggataa
     1441 tgaaggccca gatcgcccga ggggccgaac tggaaaaggg agggcccctg agaaggcttc
     1501 gggcctttat tccggttctg gtgccccttt tcgtgatcgt cttccagagg gccgatgacc
     1561 ttgccaccgc catggaggcg aggaattaca ccggcggcga aggcaggacc cggatgaacc
     1621 ctctgaaatg gaccaccagg ga
//
LOCUS       DAOSRG010000267         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_69291_length_1641_cov_4.952081, whole genome shotgun sequence.
ACCESSION   DAOSRG010000267 DAOSRG010000000
VERSION     DAOSRG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69291_length_1641_cov_4.952081"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLM30_06900"
     CDS             <1..1454
                     /locus_tag="PLM30_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOR54937.1"
                     /translation="DPLLEKRLIEGCLEIHSKGLIVAMQDMGAAGIVSSSSEIASKSG
                     AGILVNVDLVPLKEKMEPWEIALSETQERMLLIVKEEDLGEIGSIAAKWKLDWAVIGE
                     VTGDGNFVMTRGGKTVVNLPAEMVGGGSPPIHWPSKKPSMGKTVEAKSKNYLNPEEVS
                     RSLAALLEDPNLADKSGIFGRFDSEAQSNTVTPPGKSVSIFRVRENGNLVAVTMEADP
                     WKCQLEPFLGTAETFLKCLRPLWVCGAAHLGMTNCLNFPSPEDPENFWVLERSVLGLA
                     AVGGDLTCPVVSGNVSLYNESPVGKILPSPLVAVVGLLEEGRVPLGDAAELGAGSVFL
                     VGEERGFLGGSAYSRIVDPDGSYTAGSFNPVKERAFMERAHRTAREGAAAAGRVLAGG
                     GLLVSLAKICRAGGIGISLDAGNLDLSPDGLFGEWGARAVYLVPEGSTDLFLSCWEGF
                     PSRRIGKITGGVLALGEREIYPGKTAQDKVKGA"
     gene            1459..>1641
                     /locus_tag="PLM30_06905"
     CDS             1459..>1641
                     /locus_tag="PLM30_06905"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163298.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR54938.1"
                     /translation="MAGVFGAFCKNGHSVLEDVYLGLYALQHRGQESAGIAWNSNEGG
                     EVNSVKGMGLVHIALDQ"
BASE COUNT          326 a          475 c          533 g          307 t
ORIGIN      
        1 gggatcccct cctggaaaaa agactgatcg aaggctgcct cgagatccat tcgaagggct
       61 tgatagtcgc catgcaggac atgggagccg cggggatcgt ttcatcctcg agcgaaatag
      121 cctcgaaaag cggcgccggg atcctggtta acgtcgacct ggttcccctc aaggaaaaga
      181 tggagccctg ggagatagcc ctttcggaaa cccaggaaag gatgctcctc atcgtaaagg
      241 aggaggacct gggcgagatc ggctccatag ccgccaagtg gaagctggat tgggcggtga
      301 taggcgaggt caccggcgat ggcaacttcg tcatgacccg gggcgggaag accgtggtca
      361 accttccggc ggagatggtc gggggtggct ctcccccgat ccactggcct tcgaaaaagc
      421 cctccatggg gaagaccgtt gaggccaaaa gcaagaatta cctcaatccg gaagaggtct
      481 cccgctccct ggcggccctc ctggaagatc cgaacctggc cgacaaatcc ggtattttcg
      541 gccgtttcga ctccgaggcc cagtccaaca ccgtcacccc tcccggtaaa agcgtatcta
      601 tcttccgggt acgggagaac gggaacctcg tggcggtgac catggaggcg gacccctgga
      661 aatgtcaact tgagcccttc ctggggacgg ccgagacctt cctcaagtgc ctcagacccc
      721 tttgggtgtg cggtgccgcc cacctgggca tgacgaactg tctgaacttc ccgtcgccgg
      781 aagacccgga gaacttctgg gtgctggaac ggtctgtcct gggcctggca gcggtgggcg
      841 gcgacctcac ctgtcccgtt gtatcgggta atgtcagcct ctacaatgaa agccctgtcg
      901 gtaagatcct tcccagcccc cttgtcgccg tcgtggggct gctcgaagag ggaagggtcc
      961 cactgggtga tgccgccgaa ctgggagccg gctcggtctt cctggtgggg gaagaacgag
     1021 gatttttagg gggaagcgcc tactctagga tcgttgaccc agacggcagt tacacggctg
     1081 gatcctttaa ccccgtcaag gaaagggcct tcatggaaag ggcccaccgc accgcccgcg
     1141 aaggggccgc cgcggcggga cgagtgctgg ccggcggtgg gttgctggtc tccctcgcga
     1201 agatctgccg ggcggggggg atcgggatca gtctcgatgc cgggaacctc gacctttccc
     1261 ccgacggcct cttcggagaa tggggcgcga gggcggttta cctcgtcccg gaaggatcga
     1321 ccgacctttt cctttcttgc tgggagggtt tcccctcccg gaggatcggt aagataaccg
     1381 gcggcgttct cgctctcggg gagcgggaaa tataccccgg aaagacagcc caagacaagg
     1441 tcaagggggc ctagagccat ggctggggtt ttcggcgcct tttgcaaaaa cggccattcc
     1501 gtgttggagg atgtctacct ggggctttac gcccttcagc accggggaca ggaatcagcg
     1561 ggcattgcct ggaactccaa cgaaggtggc gaagtcaact ccgtaaaggg gatgggcctg
     1621 gtccatatag ccctcgatca g
//
LOCUS       DAOSRG010000268         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_69523_length_1637_cov_7.896966, whole genome shotgun sequence.
ACCESSION   DAOSRG010000268 DAOSRG010000000
VERSION     DAOSRG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69523_length_1637_cov_7.896966"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1637)
                     /locus_tag="PLM30_06910"
     CDS             complement(<1..>1637)
                     /locus_tag="PLM30_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOR54939.1"
                     /translation="RYGVEYVFGLPGETTLGLYDEWKRFEKVTHVMARDERHAVYMAD
                     GYARVSGRPGVCEGPSVGATHMVPGVVEARRSSIPLVVLTTDIPLGVEKRNALTGFDQ
                     GSLFAPFAKESLTAHRGDDVPFLVRRAFRVAVSGCPGPVHLRLPQDVLTQEVSQPLPP
                     AQERFGLCPGTRPLPCREEAAEAAQLLAACERGVMVCGQGVLTSRAWEEVERVAERFG
                     LATATTINGKGSIGELHPLSVGVIGSRGSTDFSNSFLDGAGLVFYVGCSTDSVATDGW
                     KLPREPGRDRFIHLNISEGDLGNSFGDGLLLHGDVKATLGAILEAADDLALPVKGSGA
                     PLRGRPAEGEPMAKGGCLDPRSATMMVTGAMPEGSVVVADPGMSAVYPAAFWRVPRAG
                     RYFATNYAMGALGWGLGAAIGATYAVSPGTTVLNFIGDGSFGFTSGELETAARLGRNL
                     KVVLFNNRSFGWIRATNYFSFGKPHFATDLGTVDYGALAGSYGLRSFRASTGEELALA
                     LAGFFEGEGPALLELVVPPEDECVPPVPEWGARSREEG"
BASE COUNT          251 a          584 c          563 g          239 t
ORIGIN      
        1 ccctcttccc tggaacgggc cccccattcc ggcacggggg ggacgcactc gtcctcgggg
       61 ggtaccacca gttccagcag ggccggcccc tccccttcga agaagccggc gagggccaag
      121 gccagctcct cccccgtcga ggcccggaaa gatctgagcc cgtaactccc ggcgagggcc
      181 ccgtagtcga ccgtccccag gtcggtggcg aagtggggct tgccgaaact gaagtagttc
      241 gttgccctta tccagccgaa actcctgttg ttgaagagga cgaccttgag gttcctcccc
      301 agcctggcgg cggtctccag ttccccggag gtgaagccga agctgccgtc gcctatgaag
      361 ttaaggaccg tcgtgccagg gctcacggcg taggtggccc cgatggctgc gccgagaccc
      421 caccccagcg cccccatggc gtagttggtg gcgaagtagc gcccggccct ggggaccctc
      481 cagaaggcgg cggggtagac ggcgctcatc ccggggtcag cgaccaccac ggaaccttcg
      541 ggcatggcgc cggtcaccat catggtggcc gaccggggat cgaggcaacc tcccttggcc
      601 atgggctccc cctcagcggg gcggccccgg agcggggccc cgctcccctt caccggcagg
      661 gccaggtcat cggcggcctc gaggatggcc cccagggtgg ccttcacatc tccgtgaagc
      721 aggaggccgt cgccgaagga gttgcccagg tctccctcgg atatgttcag gtggatgaag
      781 cggtcccgcc cgggctcccg ggggagtttc cacccgtcgg tggccaccga gtcggtgctg
      841 cagcccacgt agaagaccag ccccgcccca tcgaggaagg agttcgagaa gtccgtgcta
      901 ccccgggaac cgatcacgcc caccgaaagg gggtggagtt cgccgatgct ccccttcccg
      961 ttgatcgtcg tggccgtggc gaggccgaac ctctcggcga cgcgctcgac ctcctcccag
     1021 gccctcgatg tgaggacgcc ctggccgcag accatgaccc ccctctcgca ggccgcgagc
     1081 agttgggcgg cctcggcggc ctcctcccgg cagggtaggg gccgcgtccc cgggcagagc
     1141 ccgaagcgct cctgggcggg cggcagcggc tggctgacct cctgggtcaa cacgtcctgg
     1201 gggagcctca ggtgcaccgg cccggggcac cccgatacgg ccacgcggaa agccctgcgg
     1261 accaggaagg gcacgtcgtc gccgcggtgg gccgtgaggc tctccttcgc gaagggtgca
     1321 aagagggacc cctggtcgaa gcccgtgagg gcgttgcgct tctccacccc cagggggatg
     1381 tccgtcgtca gaaccaccaa gggtatggag gaccggcggg cctcgacgac gccggggacc
     1441 atgtgggtgg ccccgacgct cggcccctcg cagacgccgg ggcggccgct gacgcgggcg
     1501 tacccgtcgg ccatgtacac cgcgtggcgc tcgtcacggg ccatgacgtg ggtgaccttt
     1561 tcaaacctct tccactcgtc gtaaaggccc agggtcgtct cgccggggag gccgaagacg
     1621 tactccaccc cgtagcg
//
LOCUS       DAOSRG010000269         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_70146_length_1627_cov_5.788168, whole genome shotgun sequence.
ACCESSION   DAOSRG010000269 DAOSRG010000000
VERSION     DAOSRG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70146_length_1627_cov_5.788168"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..951
                     /locus_tag="PLM30_06915"
     CDS             157..951
                     /locus_tag="PLM30_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOR54940.1"
                     /translation="MERLRVGLLQLEVIREDAARNARYVLGQIRDGARSGCDVLVLPE
                     LWNVAFPLPGPHVLIDEESPLVGSIAEAACRNETWIIGGSMALSTAAGPRNRCLVFSP
                     EGDIAFRYDKVHLYPGLDEPNILSPGVSPGSFDMGGLPCGVMICFDAEFPELATSLSE
                     GGAKVLFVPAAWKAEFTRLWRALLISRAIENQVFVVGVNRCDRGKNSSFGGQSLVVDP
                     FGDLILHLDDTPRFERVILDLSRVEVARSDHKVVSSRRPEIYRRWR"
     gene            1225..>1627
                     /locus_tag="PLM30_06920"
     CDS             1225..>1627
                     /locus_tag="PLM30_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOR54941.1"
                     /translation="MSNETQREQWGTRWGYILACAGSAVGLGNIWRFPYITGLHGGAA
                     FVLIYVILVFVIGFTVMLAEMAIGRNSQLNAVGAFRKIKGGAWPIVGWMGVTAGFIIL
                     SYYGVIGGWTMAYVLKSFTGLMQADAGEAFGS"
BASE COUNT          307 a          460 c          498 g          362 t
ORIGIN      
        1 aggcgatcac cctgtgaggg atggccaggc acatcgccgc tccactccct tccctcggac
       61 cgttgccaac gggcaccggt ccgactttca ccccagttta tcattgaact tgccccttcg
      121 tatcccagag ggtagtctag caccgagggg acgagcatgg aacgattgag ggtgggcctt
      181 cttcaacttg aggtgatccg agaggacgcg gccaggaacg cgaggtacgt gctcggacag
      241 atccgcgacg gggcgaggag cggttgtgac gtgctggttt tgccggaact gtggaacgtc
      301 gccttccctc tcccaggacc ccatgtcctt atcgatgaag aaagtcccct tgtggggtcc
      361 atcgccgagg cggcctgccg gaacgagacc tggataatcg ggggcagcat ggccctgtca
      421 accgctgccg ggccgaggaa tcgatgcctt gtcttctctc ccgagggaga tattgccttc
      481 cgctacgaca aggtccacct ctatcccggc ctggacgaac ccaatattct ctcccccggc
      541 gtgtcccccg gctcttttga tatgggaggg cttccctgcg gcgtcatgat atgttttgac
      601 gcggagttcc cggaattggc gacgagcctt tcagagggcg gagccaaggt ccttttcgtg
      661 ccggccgcgt ggaaggccga gttcacccgc ctttggaggg cccttctcat ttccagggcc
      721 atcgagaacc aggtcttcgt cgtcggtgtc aaccggtgcg acagggggaa aaactcctcc
      781 ttcggggggc agagcctcgt ggtcgacccc tttggtgacc tgatcctgca cctggacgac
      841 acgccccgtt tcgagagggt gatccttgac ctttccaggg tggaggtcgc caggagcgat
      901 cacaaggtgg tctcttcaag gagacctgag atctaccggc gatggcgtta actgagacat
      961 aatgaacctt ccccattgac tggctcgccc tttgttatat aatgcgctgc aatcatgacc
     1021 ttgagggact ttaaaacctt cccgggccaa ggtgctgttg cgtttgccaa aagaatgggt
     1081 gggctttata attccatcag gtttatgagg taaagcgaag gaggtgtccg cgcggaaggg
     1141 aggcaaaggg agtatccagt cgagtgagcc ttgaggtcag ttttgaggta atccaagcct
     1201 agtttcaaga ggagggatat cagcatgagc aacgagacgc aacgcgaaca gtggggtacc
     1261 aggtggggat acatccttgc ctgcgccggc tccgcggttg gcctggggaa catctggcgt
     1321 ttcccttaca tcaccggtct gcatggaggg gcggcctttg tcctgatcta tgtgatcctt
     1381 gtcttcgtta tcgggttcac cgtcatgctc gccgaaatgg caatcggccg caactctcag
     1441 cttaacgccg tcggagcctt ccgaaagatc aagggcgggg cgtggcccat cgtgggctgg
     1501 atgggagtaa ccgccggctt catcattctc tcttactacg gggtcatcgg cggctggacc
     1561 atggcctacg tgctcaaatc cttcaccggg cttatgcagg ctgacgccgg ggaggccttc
     1621 ggttcct
//
LOCUS       DAOSRG010000270         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_71085_length_1613_cov_9.725289, whole genome shotgun sequence.
ACCESSION   DAOSRG010000270 DAOSRG010000000
VERSION     DAOSRG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71085_length_1613_cov_9.725289"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLM30_06925"
     CDS             complement(<1..539)
                     /locus_tag="PLM30_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR54942.1"
                     /translation="MKGRGIEASLLAWRDVAAGDFASEALRKRTETLPETERTLATTL
                     VFAALRRAALWKLMARELTGRPLGAMSRAAGDALVLGIAGVCELKHFSPRVLVNALVE
                     WVKEKGEGREPGMVNAVLRRAARESPDHVASLRSSRLFRDACLYSGIPGRVGALWAAS
                     WGRELAKEIVRLASMKAYLS"
     gene            complement(536..1231)
                     /locus_tag="PLM30_06930"
     CDS             complement(536..1231)
                     /locus_tag="PLM30_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOR54943.1"
                     /translation="MPLVLLLLFFLFFVPWLGFLILALSLFLFLLVPLGFAARSLAWL
                     VIGPRELYKVLSDRRVRKNHALEHGTINILEQQYGLPGLTGRAREDGFGLSGLPNPQL
                     ILETAELARERLAAGETRLAIHRRCGTTMVLVNLSSSVIFILLLVVAGRFSFGTVLLS
                     LAAAWVIGNWASPLVQRHLTTDSDVGDLVITGAEARLRPVRFFGGTAMLPAEVFVSTR
                     SATGPILAEVVRP"
     gene            complement(1353..>1613)
                     /locus_tag="PLM30_06935"
     CDS             complement(1353..>1613)
                     /locus_tag="PLM30_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOR54944.1"
                     /translation="SNPTMMNVGILFFVAVLLFHLVTLPVEIDASRRALSILSSSGLL
                     ATDEIGGAKAVLNAAALTYVSATLMSALQLVRLFLLRGMRNR"
BASE COUNT          346 a          521 c          479 g          267 t
ORIGIN      
        1 gaaaggtagg ccttcattga cgcgagcctc acgatctcct tcgccaattc ccgcccccac
       61 gatgccgccc agagagcgcc caccctgccc ggtatcccgg aatagaggca ggcatccctg
      121 aaaagccggc tcgatctcag gctggccaca tgatcggggc tctccctggc cgctctgcga
      181 aggacggcat tcaccatccc cggctcccgg ccttccccct tttccttgac ccattccacc
      241 agggcattga ccaggaccct gggcgagaaa tgcttcagct cgcagacgcc ggctatacct
      301 agaacaagag catccccggc cgcccggctc atcgctccaa ggggtcttcc ggtcagttcc
      361 cgagccataa gcttccagag agcggccctc ctgagggccg cgaaaaccag cgtcgtcgcc
      421 agggttcttt ccgtttcggg aagggtttcc gtcctcttcc tgagggcttc gctggcgaag
      481 tctcccgcgg cgacatctct ccaggccagg agcgaagctt ctatgcccct gcctttcaag
      541 gcctgaccac ctcagccagg atgggcccag tggcggacct ggtgctgacg aagacttcgg
      601 cgggcagcat cgcggtacct ccgaaaaagc ggaccggccg gagcctcgct tcggccccgg
      661 taataacgag gtcccccacg tcgctgtcgg tagtcaagtg acgctggacc agggggctgg
      721 cccagttgcc gatgacccag gctgccgcca gggagagcag gaccgtcccg aaggaaaacc
      781 gcccggcgac caccagcagg aggatgaata tcaccgagga ggagaggttc accagcacca
      841 tcgtcgtgcc gcacctgcgg tgtatggcca gtcgggtttc accggctgcg agcctttccc
      901 tggccagttc cgccgtttcc agtatcaact gggggttcgg caaaccgctc agcccgaagc
      961 catcttcccg ggccctgccg gtcaggcctg gaagcccgta ctgctgttca agaatattga
     1021 tcgttccatg ctccagggca tggtttttcc tcactctcct gtccgaaagg accttgtaaa
     1081 gctccctggg gccgatcacg agccaggcga gggacctcgc cgcgaacccc aggggaacca
     1141 gcaggaagag gaacagggaa agggcgagga tgaggaatcc cagccaaggt acgaaaaaca
     1201 ggaagaaaag caacagaaga accaggggca tggggcagat cgatctcctc tcagccatcc
     1261 taggccctga ccgggagcgt caaagcgaat aggggggcct cagccccccc tatgatacct
     1321 caaaccgcaa aagccaaaga tcggggcgat gcctagcggt tcctcatccc cctcagaagg
     1381 aaaagcctca ccagttggag ggcagacatt agggtggcag acacgtaagt aagggcggca
     1441 gcgttcagga cggctttcgc accgccgatc tcgtccgtgg cgaggagccc ggaactggag
     1501 agtatggaaa gagccctcct gctcgcgtcg atctccaccg ggagggtaac aaggtggaaa
     1561 aggaggaccg ccacgaagaa gagtatcccc acgttcatca tcgtagggtt gga
//
LOCUS       DAOSRG010000271         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_71349_length_1609_cov_8.084942, whole genome shotgun sequence.
ACCESSION   DAOSRG010000271 DAOSRG010000000
VERSION     DAOSRG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71349_length_1609_cov_8.084942"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLM30_06940"
     CDS             complement(<1..197)
                     /locus_tag="PLM30_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR54945.1"
                     /translation="MTEEETRNAVQLGEEVGGRRREKGLKLSEISERTKIRLPFLEAI
                     EEGSLQELPQSIYARGFVKAYL"
     gene            complement(190..894)
                     /gene="rpe"
                     /locus_tag="PLM30_06945"
     CDS             complement(190..894)
                     /gene="rpe"
                     /locus_tag="PLM30_06945"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOR54946.1"
                     /translation="MAGPLSVRGKERPIIAPSLLSADPLDIGGSVDSLREGFDWLHLD
                     VMDGHFVPNLSYGPALVEACRRRWPDTVIDVHLMVEKPGYFLEAFAKAGSTILTVHQE
                     TTPHLHRVLQEIRDLGCSPGVTLNPGTPVETLTAVLGLVDLVLVMSVNPGFGGQAFIP
                     GSLEKARLLCCWREALGCDYLVEIDGGIGPDNAARVVAGGCDVLVAGSAIFNTPEPHE
                     AVRKIRARALEGVLRD"
     gene            complement(873..>1609)
                     /locus_tag="PLM30_06950"
     CDS             complement(873..>1609)
                     /locus_tag="PLM30_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOR54947.1"
                     /translation="QESGFDVGDVVKVHSDSRPAGVIVAQSPASPVMVPPGRRIDLLV
                     SLGPPRTGGKVVVPDVLERSEKIARQIIVQSGLKVGSVKYEYTYMTPAGMVMNMDPKP
                     GKTLAPDGVVNLVVATGKKPEELAPQASSPAEEERETVTFPTEVAPASPPVPGPAVSQ
                     EKPTAPPAPAPSPGDKIARIRYQVPPLARSMPVRIEIVDGNGTRVLLDRQASAGEYIS
                     LDAPYVGQSSVTIYLGGEFVWQDRYQ"
BASE COUNT          304 a          529 c          463 g          313 t
ORIGIN      
        1 aggtaggctt tgacgaatcc cctggcatag atactctggg gcagctcctg gagagaacct
       61 tcctcaatgg cctccaggaa gggcaagcga atctttgtcc tttcggatat ttccgaaagc
      121 ttcaggccct tttcccggcg gcgcccgccg acttcctcac ccagctgtac agcgttcctt
      181 gtctcttctt cagtcacgga gcactccttc cagggccctt gccctgatct tcctcacggc
      241 ttcatgtggc tcgggagtgt tgaagatggc actgccagct acgagcacat cgcacccccc
      301 tgcgacgacc cgggccgcgt tatcgggccc tatacccccg tcgatctcaa cgaggtaatc
      361 gcagccgagg gcttccctcc agcaacacaa tagccttgcc ttttccagcg acccgggtat
      421 gaaggcctgc cccccgaagc cagggttgac cgacatcacc aggacaagat cgacgagccc
      481 caggacagcc gtcagggtct caaccggggt cccgggattc aaagtaacac cgggagagca
      541 acccaggtct ctgatctcct gaagcacgcg atgaagatga ggggtcgttt cctgatggac
      601 cgtcaggatc gtcgacccag ccttggcgaa ggcctccaga aagtacccgg gcttttctac
      661 catgaggtgg acatcgataa ccgtatcggg ccagcgccta cggcaggcct cgaccaacgc
      721 cgggccgtag gataggttgg ggacgaaatg cccgtccatg acgtcgaggt gcaaccagtc
      781 aaatccttct cgaagagagt caacagaacc cccaatgtcc agggggtccg ccgagagcag
      841 agagggggct atgatcggcc tctccttccc ccttactgat agcggtcctg ccacacgaat
      901 tcccccccaa ggtaaatggt caccgacgat tgccctacgt agggggcgtc cagggatata
      961 tactctcctg ccgaggcctg ccggtcgaga aggaccctgg ttccgttccc atccacgatc
     1021 tcgatcctca ccggcatgga ccgggcgagg ggtgggacct ggtaccggat cctcgcgatc
     1081 ttgtcacccg gggaaggcgc cggagctgga ggagctgtcg gcttctcctg gctcacggcc
     1141 ggccctggga ccggtggaga ggcaggagcg acctccgtgg ggaaggtgac cgtctctcgc
     1201 tcctcttccg caggagatga agcttgcggg gccagctcct caggcttctt tcccgtggcc
     1261 accacaaggt tgaccacccc gtcgggagca agggtctttc cgggcttggg atccatattc
     1321 atgaccatcc ccgccggtgt catgtaggtg tactcgtact tgacggaccc gactttcagc
     1381 ccgctctgaa cgatgatctg cctggcgatc ttctcgctcc tctcgaggac atcaggcacg
     1441 acgaccttcc ccccagtcct gggggggcca aggctgacca ggaggtcgat cctcctgccg
     1501 ggcggtacca tcaccggcga cgccgggctc tgcgcgacga taacacccgc cggtctgctg
     1561 tcgctgtgga ccttgaccac gtcacctacg tcgaatcccg attcctgga
//
LOCUS       DAOSRG010000272         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_71537_length_1606_cov_7.932302, whole genome shotgun sequence.
ACCESSION   DAOSRG010000272 DAOSRG010000000
VERSION     DAOSRG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71537_length_1606_cov_7.932302"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLM30_06955"
     CDS             <1..822
                     /locus_tag="PLM30_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOR54948.1"
                     /translation="SALAMEEAGVDLVLFAGGDGTARDIAAVLGTRVPALGIPSGVKI
                     HSPVFARNPTMAGELAGLFLSGGSVVLEEREVMDIDEEAFREGSLRARLFGYLLVPCE
                     RRFLQGGKSGAGKSGAVELDGIAREVISRMVPGDLYLVGPGTTTAAIMKRLGCEYSLL
                     GVDALRDGKTLGVDLSEREVLDLLMPGRTWLVLSVIGGQGFILGRGNLQLSPGVIKSV
                     GKERILVLGAPSKLKDLYGKALLVDTGDPSLDEALCGYAPVITGLGEETLFRVER"
     gene            complement(862..1410)
                     /locus_tag="PLM30_06960"
     CDS             complement(862..1410)
                     /locus_tag="PLM30_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR54949.1"
                     /translation="MKRILGILGSPRRGGNSETLARKVLEGAAGNEWVTDVIRLQDLE
                     INGCTDCRRCWSGGSPCVIEDDMSKVYEKVREADLLVFASPLYWYSWSSQIKPIWDRF
                     LPFVHKEARWDLKDKRALLVAAAGDDKPEAFEGMLFSFRTSCRLLGMEMVKPLLAHGV
                     HGMGEAEKGHWLRLAEELGRSL"
     gene            complement(1407..>1606)
                     /locus_tag="PLM30_06965"
     CDS             complement(1407..>1606)
                     /locus_tag="PLM30_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOR54950.1"
                     /translation="QLGLIGINQAVTRRFGVPGLKAAMDHAGLYGGPVRAPLLPAEEK
                     VRKEIGRIYDQFRSFYKGVSS"
BASE COUNT          280 a          486 c          521 g          319 t
ORIGIN      
        1 tcggccctcg cgatggagga ggccggcgtc gacctggtcc tcttcgccgg gggtgacggg
       61 accgccaggg atatcgcggc cgtattggga acgagagtcc cggcgctggg tatcccgtcg
      121 ggggtcaaga tccactcccc cgtattcgcc cgtaacccga ccatggcggg agaactggcc
      181 ggtcttttcc tttccggagg gagtgtcgtg ctcgaggagc gggaggttat ggatatcgat
      241 gaagaggcct tcagagaagg gtccctccgg gccaggctct tcggttacct gctggtgcct
      301 tgcgagagga ggttcctcca gggggggaag tcgggtgccg ggaagagcgg ggccgttgaa
      361 ctcgacggga tagccaggga ggtcatctcc aggatggtcc cgggggacct gtacctggtc
      421 ggcccgggga ccaccacggc ggcgatcatg aaaaggctgg ggtgcgaata ttccctgttg
      481 ggggtcgatg ccctcaggga cggcaagacc ctcggggtcg acctgtcgga gagggaggtc
      541 ctggacctgc ttatgcccgg gaggacctgg ctggtgctct ccgtcatagg cggccagggt
      601 ttcatcctgg ggcgggggaa cctccagctg tcgccagggg tgatcaagtc cgtgggaaaa
      661 gagaggatcc tggtccttgg ggccccttcc aaactcaagg acctttacgg caaggccctc
      721 cttgtcgata cgggcgaccc ttcgctggat gaggctttat gcgggtacgc cccggtgatc
      781 accggtcttg gcgaggaaac actgttccgg gtggaacgat aaaaaggggg cggtaaaggg
      841 gccgccccct tgccgcttct cttaaagcga tctcccgagt tcctcagcta gcctgagcca
      901 gtggcccttt tccgcctctc ccatgccatg gacgccgtgg gcgagcagag gtttcaccat
      961 ttccatgccg aggagtctgc aggatgtcct gaatgaaaag agcattccct cgaaggcctc
     1021 aggcttatca tcaccggcgg cggccaccag cagggccctc ttgtccttca ggtcccagcg
     1081 ggcctcctta tgaacgaagg ggaggaacct gtcccagatg ggcttgatct gggaactcca
     1141 ggagtaccag tagaggggtg aggcgaagac caggaggtcg gcctcccgga ccttctcgta
     1201 gaccttgctc atgtcgtcct cgatcacgca gggtgaccct cccgaccagc acctcctgca
     1261 gtcggtacaa ccgttaatct ccaggtcctg gaggcgtatg acatccgtga cccactcgtt
     1321 tcccgccgcc ccttccagca ctttccgagc cagtgtctcg ctgttgcctc cccttcgggg
     1381 actgcccagt attccaagga tccttttcat gatgaaaccc ccttgtaaaa agatctgaac
     1441 tggtcgtaga tcctcccgat ctctttccgg accttttcct cggccgggag cagcggcgcc
     1501 ctgacggggc cgccgtaaag accggcgtgg tccatggcgg ccttcagccc aggcactccg
     1561 aagcgcctgg tgaccgcctg gttgatcccg atgaggccga gctgca
//
LOCUS       DAOSRG010000273         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_72132_length_1597_cov_5.297017, whole genome shotgun sequence.
ACCESSION   DAOSRG010000273 DAOSRG010000000
VERSION     DAOSRG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72132_length_1597_cov_5.297017"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLM30_06970"
     CDS             <1..490
                     /locus_tag="PLM30_06970"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOR54951.1"
                     /translation="VASVASRVMNRRDASPGLPLEDLQDALESEFLARYPASGFQREQ
                     EMPGGEEFRRLARRYRSRKWRYGRSPGCEVSLRRRFPWGGVEIRFDVRDGRVASSVVF
                     SDAMDGEMILALAETLEGIPFRWKEMADAIRRGFPDRQEMTETAAWLEEAPIGERKEA
                     CS"
     gene            487..>1597
                     /gene="gcvT"
                     /locus_tag="PLM30_06975"
     CDS             487..>1597
                     /gene="gcvT"
                     /locus_tag="PLM30_06975"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOR54952.1"
                     /translation="MKRTDMYERHVAAGGRMVDYSGWELPIQYDGTGIKTEHLAVREK
                     AGLFDVSHMGEIMAEGPDAEKWLSTLVTNNIFVMKDYQVQYNIMCNETGGVVDDLLVY
                     RYNPEKFLLVVNAANVDKDWKWLKKHLPVKGVAIDNISMKTSETALQGPAAEAILAKV
                     ADFDPSTLKFFTFTDKADLNGIPAIVSRTGYTGEDGFEIYVPWERGAELWDLLIETGR
                     DFGLIPIGLGARDSLRFEAGLPLYGHEYSEDLGPLEAGFGFFVKADKPGGFIGQPVLK
                     RQKEEGLRRKIVGLRLEDKGVPRSGMDVSDASGNRVGKVTSGGYCPSLDANMALCLVK
                     TPAPEEGEHLSVLIREKPKKGIVVKRPFYDRKNGKR"
BASE COUNT          340 a          472 c          540 g          245 t
ORIGIN      
        1 cgtggcctcg gtggcctccc gggtgatgaa ccgcagggac gcctcgccgg ggctgcccct
       61 ggaggacctg caggacgccc tcgaaagcga gttcctcgcc cgctaccccg cctcggggtt
      121 tcaaagggaa caggaaatgc ccggagggga ggaattccgg cgcctcgcga ggcgttaccg
      181 gagccggaag tggcgctacg gtcgctcccc cggctgcgag gtatcgcttc ggcgtcgttt
      241 cccctgggga ggagtggaaa tccgcttcga tgtaagggac ggccgggtcg cctcgtcggt
      301 ggtcttttcc gacgcgatgg acggcgagat gatcctggcc ctggcggaaa cgctcgaagg
      361 catacccttc cgatggaagg agatggccga cgcaatccga aggggcttcc cggaccggca
      421 ggaaatgacc gaaacagccg catggctcga ggaggccccg ataggcgaaa ggaaggaggc
      481 atgttcatga agcgcaccga catgtacgag aggcacgtag cggcaggtgg aagaatggtc
      541 gactacagcg ggtgggaact gccgatccag tacgatggca ccggcatcaa gacggagcac
      601 ctggccgtca gggaaaaggc ggggctcttc gatgtctccc acatgggaga gatcatggcg
      661 gagggccccg acgcggagaa atggctctcc actctcgtga cgaacaatat attcgtgatg
      721 aaggactacc aggtgcagta caacatcatg tgcaacgaga cggggggcgt cgtggacgac
      781 ctgctcgtct accggtacaa cccggagaag ttcctcctcg tggtgaacgc ggccaacgtg
      841 gacaaggact ggaaatggct caagaagcac ctacccgtga agggcgtggc catcgacaac
      901 atctccatga agacctcgga gacggcgctc caggggccgg cggcggaggc catcctggcc
      961 aaggtcgccg acttcgaccc gtcgaccctc aaattcttca ccttcaccga caaggccgac
     1021 ctgaatggca tccccgccat tgtaagcagg accggctaca ccggcgagga cggtttcgag
     1081 atctatgtcc cctgggaaag aggcgccgaa ctatgggacc tcctcatcga gacgggccga
     1141 gacttcggcc tgatccccat cgggctcggc gcccgggaca gcctgcgctt cgaggcgggg
     1201 ctcccgcttt acggtcacga gtattccgag gacctggggc ccctggaggc cggcttcggt
     1261 ttcttcgtca aggcggacaa gccgggcggt ttcatcggcc aaccggtgct gaagcgccag
     1321 aaggaagaag ggcttcggcg gaagatagtc gggctacggc tcgaggacaa gggggtgccc
     1381 cgctccggca tggacgtgtc cgacgcctcg ggaaacaggg tgggcaaggt gacctccggg
     1441 ggatactgcc cctccctgga cgccaacatg gccctctgcc tcgtcaagac cccggcgccg
     1501 gaggaaggag aacacctcag cgtcctgatc agggaaaaac cgaagaaggg catagtggtg
     1561 aaaaggccct tctacgacag gaaaaacggg aaacggt
//
LOCUS       DAOSRG010000274         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_72198_length_1596_cov_6.707333, whole genome shotgun sequence.
ACCESSION   DAOSRG010000274 DAOSRG010000000
VERSION     DAOSRG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72198_length_1596_cov_6.707333"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1596
                     /locus_tag="PLM30_06980"
     CDS             114..>1596
                     /locus_tag="PLM30_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:465..467,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR54953.1"
                     /translation="MDELTGKIWNVKTDYDSTTSMNAGRCCVKGRFGWSFVHSDDRLK
                     KPLIKEKGAFREASWDEALGLVAEKFTGFRDSSGPDSVAFFSSARCTNEENYLLQRFA
                     REVMGTNNIDHCAHLUHSATVAGLGETLGSGAMTNDYESIKHADVALVIGSNTTENHP
                     VIGSWLKEQQKNGRTKLIVCDPRRIELDDYADIKVRHRSGSDVALLNGLMNIIIAEGL
                     HDKEFIDKNVEKFDELKALVAEYTPAKVSAITGVPEETLKEAARLYARGPNSAIFYTM
                     GITQHQYGTNNVKTVADLALLCGMLGRPGTGVNPLRGQNNVQGACDMGCLPASLPGYL
                     DIAKNTEKAAEKVRQIWKAELPTSPGRTIVKVTDGLAKGEVKGLFIMGENPMVSDPNT
                     NHVKDALEKAEFLVVQDIFMTETAQLADVVLPAACWAEKDGTFTNTCRAVQRIRKAVE
                     APGDARSDWEIIVDLAKACGADWDFHSPEDVMEEISRFVPQYGG"
BASE COUNT          382 a          480 c          479 g          255 t
ORIGIN      
        1 cgacgggggc cctgacggag aactcggtgc acggcatcag ccgcccttcc aaaactaggg
       61 tcgtcaagac catctgcccc tactgcgggg taggttgcga actggacatc cacgtggacg
      121 aactcacggg caagatctgg aacgtgaaga ccgactatga cagcaccacc tcgatgaacg
      181 cgggaagatg ttgcgtgaaa ggccggttcg gttggagctt cgtccacagc gatgacaggc
      241 tcaaaaagcc cctgataaag gaaaagggtg ctttcaggga ggcatcgtgg gatgaagccc
      301 tgggcctggt ggccgagaag ttcactggct tcagggattc cagcggcccg gactcggtgg
      361 ccttcttctc ctcggcacgc tgcaccaacg aggagaacta cctgctccag cgattcgcga
      421 gggaggtgat gggcacaaac aacatcgacc actgtgccca cctctgacac tccgctactg
      481 ttgcgggtct cggcgagacc ctcggaagcg gggcaatgac caacgattac gagtcgataa
      541 agcacgccga cgtggccctg gtcatcgggt ccaacacgac ggagaaccac cccgtcatcg
      601 gctcatggct taaggaacag caaaagaacg gccggaccaa gctgatagtc tgtgacccga
      661 gaaggatcga gctggacgac tatgccgaca tcaaggtcag gcaccgttcg gggagcgacg
      721 tggccctcct taacgggctc atgaacataa tcatcgccga agggttacac gacaaggaat
      781 tcatcgacaa gaacgtcgaa aaattcgacg aactgaaggc ccttgtcgcg gaatacaccc
      841 ccgccaaggt gagcgccatt accggggtgc ccgaggagac cctgaaagaa gcggcccggc
      901 tctatgcccg tggccctaac tcggcgatct tctacaccat gggcatcacc cagcaccagt
      961 acggcaccaa taatgtaaaa accgtggccg atctcgctct gctctgcggc atgctcggaa
     1021 ggcccggcac gggcgtcaac cccttgcggg gccagaacaa cgtccagggc gcctgcgaca
     1081 tgggctgcct gccggcgtcg ctcccgggtt acctcgacat cgccaagaac accgaaaaag
     1141 ccgctgaaaa agtgaggcag atctggaagg ccgagcttcc cacctccccg ggcaggacca
     1201 tcgtcaaagt caccgacggg ctcgccaagg gcgaggtcaa ggggctgttc atcatgggag
     1261 agaaccccat ggtcagcgat cccaacacta accacgtcaa ggacgctctc gaaaaggcgg
     1321 aatttctcgt cgtccaggac attttcatga ccgagacggc ccagctggcc gacgtggtac
     1381 tcccggcggc ttgctgggcg gaaaaggacg gcaccttcac gaatacctgc agagccgtgc
     1441 aaagaatacg caaggccgtc gaggcccccg gcgacgctcg ctcagattgg gagatcatcg
     1501 ttgacctggc caaagcctgc ggagcggact gggacttcca ctcccccgag gacgtaatgg
     1561 aggagataag caggtttgtg ccgcagtacg gcggta
//
LOCUS       DAOSRG010000275         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_72262_length_1595_cov_8.300649, whole genome shotgun sequence.
ACCESSION   DAOSRG010000275 DAOSRG010000000
VERSION     DAOSRG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72262_length_1595_cov_8.300649"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="PLM30_06985"
     CDS             <1..>1595
                     /locus_tag="PLM30_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR54954.1"
                     /translation="EMRALLLKIIALSWELFRAAKSAAGTLSFDDMISRALQVVTAEP
                     SLPGRFLHVIVDEFQDTNALQDDLLSALVPPGKGSLFLVGDLQQSIYRFRHAEPAIFL
                     KRLMEAFQSNPDSLVNLDVTFRSRQAVMDAVNSLFGRVWREGVTKALESGFSRLAPPE
                     SRDWWPKRQETTVKPFEIVIPAGEAPPAKPRVSEIRLSAMRLLADRIVGAVDSGATVW
                     DTDGEGSFAPRPARFRDFAVLVPSRTFYDQIEQVFIDERGIPTYFEGNRNYFGRGEVR
                     DAVRLLEALADPGDPLAMASFLASPLSGLAPGVSARLIAKSRSDGSDLLSLLAQEHPL
                     EASRFERLRREGLAAGPSRPLATLLEDDSALLSCEGWKRPRVAANLRRAIDLAREYEA
                     GLGMSLGGCAGYLRDMTRRGIETREADTLGEDDDMVRVMTVHAAKGLEFPVVAVTGLE
                     LAPRTRKQGVRLVVSPHLGIAATALPEGWVEVGEGEVLAGAIHDLFENRETWEENQRL
                     LYVACTRARDSLILCGASPRTEEG"
BASE COUNT          259 a          547 c          542 g          247 t
ORIGIN      
        1 gagatgaggg ccctgctgct caagatcatc gccctctcct gggaactgtt ccgcgccgcc
       61 aagtcagccg cggggaccct gtccttcgac gacatgatca gccgggccct gcaggtcgtg
      121 acggcggaac cctccctccc gggcaggttc cttcatgtca tcgtcgacga gttccaggac
      181 accaacgccc tgcaggacga cctcctctcg gccctcgtac ccccaggcaa gggcagcctc
      241 ttcctggtgg gcgaccttca gcagtccatc taccggttcc gccatgccga gccggccatc
      301 ttcctgaaaa ggttgatgga ggccttccaa agcaaccccg actccctggt gaacctcgac
      361 gtgaccttcc gctcgaggca ggccgtcatg gacgccgtca acagtctctt cggccgcgtg
      421 tggagggagg gcgtcaccaa ggccctggag agcgggttct cccggctagc acccccggaa
      481 tcgcgggatt ggtggccgaa acgccaggag accaccgtga aacccttcga gatcgtcatc
      541 cccgcgggcg aggcgccgcc cgcgaagccg agggtcagtg aaatacgcct gtcggcgatg
      601 cgcctcctcg ccgaccggat cgtcggggcc gtggactcgg gggccaccgt gtgggatacc
      661 gacggcgagg ggtccttcgc gcccaggccg gcccgtttcc gggatttcgc cgtgctggtg
      721 ccgtcgagga ccttctacga ccagatagag caggtcttca tcgatgaaag gggcataccc
      781 acctacttcg agggcaaccg gaactacttc ggccggggcg aggtcaggga cgccgttagg
      841 ctcctcgagg ccctggccga ccccggcgac ccgctggcca tggcctcctt cctggcctcg
      901 ccgctgagcg gcctggcgcc gggagtatcg gcccggctca tcgcgaagtc gcggtcggac
      961 ggctccgacc tcctgtccct cctggcccaa gaacaccctc ttgaagcttc gcgtttcgaa
     1021 aggctccgga gggaagggct cgcggcgggt ccgtcccgcc cactggccac cctgctcgag
     1081 gacgattcgg ccttgctctc ctgcgagggc tggaagcggc cccgggtggc ggccaacctc
     1141 agaagggcca tagacctggc ccgggagtac gaagccggcc tgggcatgag cctcgggggc
     1201 tgcgcgggct acctccgcga catgacccgc agggggatcg agaccaggga ggccgacacc
     1261 ctcggcgagg acgacgacat ggtgagggtc atgacggtcc acgccgcgaa ggggcttgag
     1321 ttccccgtgg tggccgtgac ggggctcgaa ctggcgccga ggacccggaa acagggggtg
     1381 aggctcgtgg tctcccccca cctgggcatc gccgccacgg ccttgccgga gggctgggta
     1441 gaggtcgggg agggggaggt cctcgcgggg gccatccacg acctgttcga aaaccgggag
     1501 acctgggagg aaaaccagag gctcctctac gtggcctgca ccagggcgag ggactcgctc
     1561 atcctctgcg gggcctcccc ccggaccgag gaagg
//
LOCUS       DAOSRG010000276         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_72457_length_1592_cov_8.734548, whole genome shotgun sequence.
ACCESSION   DAOSRG010000276 DAOSRG010000000
VERSION     DAOSRG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72457_length_1592_cov_8.734548"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLM30_06990"
     CDS             <1..878
                     /locus_tag="PLM30_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54955.1"
                     /translation="GNPAFADSFQGRIFPPFGCQRQTDLRGDYSATIMTRGFAFYSYL
                     TSTRQPGEILSELKAVARQALEGSIGQYRKNAGEFIARGGSLSGLREWEPAVLSFAEL
                     NRLVREALGEGYERFVAAALEAAPPGADERDLAARLVGAMVDRCGPAAPVVVVGFLPP
                     WYPHRANLGASAGERVVEGAALEAVREMKGRFGLDLEVRPFFEGVSDLSYCGFQGDDR
                     EMGVFAGNMPGWGRLYRLPIEALAELDIPILNLGAVAKDSHKCTERVHLPYMLEVYPE
                     ILRFVVKEIIEGYRS"
     gene            996..>1592
                     /locus_tag="PLM30_06995"
     CDS             996..>1592
                     /locus_tag="PLM30_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR54956.1"
                     /translation="MAYMTLWLKKVSRRVQEFRLTPRILKEWALIHFGCFLFAAGVTW
                     FLVPNNMAAGGISGFAMVINHFFPNMPVGVLMMAMNVILFIIGFIFIGFEFGAKTIYS
                     SLFLSGAVWFLERFFPVSGPLSDDIMIQLIFGTLLGATGMAILFNQNASTGGTDITAK
                     ILNKYLNIDLGKGVLLCDFSYVLAAAWVFGIRIGMYAAL"
BASE COUNT          278 a          481 c          501 g          332 t
ORIGIN      
        1 acggcaaccc tgccttcgcc gactccttcc aggggaggat cttcccgccc ttcggctgcc
       61 agaggcagac cgacctccgg ggggattact cggccaccat catgaccagg ggcttcgcct
      121 tctacagcta cctgacgtcc acgaggcagc cgggggagat actctccgaa ctgaaggccg
      181 tcgcccgaca agctctggag ggttccatcg ggcaataccg gaagaacgcc ggggagttca
      241 tcgcccgggg cggatccctg tcgggcctcc gcgaatggga acccgcggtg ctctccttcg
      301 ccgaactcaa ccgcttggtg agggaggccc tgggagaggg ttacgaaaga tttgtggcgg
      361 cggcgctgga ggcggccccg ccgggggccg acgagaggga cctcgccgcc cggctggtcg
      421 gcgcgatggt ggaccgctgc ggcccggccg cccccgtcgt ggtggtgggt tttcttcccc
      481 cctggtaccc tcacagggcc aacctgggcg cgagcgccgg cgagagggtg gtcgagggag
      541 cggccctcga ggcggtccgg gagatgaaag ggcgtttcgg ccttgacctc gaggtaaggc
      601 ccttcttcga gggcgtctcg gacctgagtt attgcggttt ccagggcgat gaccgggaga
      661 tgggggtctt cgccggcaac atgcccggct gggggaggct gtaccggctc cccatcgagg
      721 ccctggccga actggacata ccgatcctga acctgggtgc cgtggccaag gactcccaca
      781 agtgcaccga gcgcgtccac ctgccctaca tgctcgaggt atacccggag atcctgcgtt
      841 tcgtcgtgaa ggagatcatc gagggatacc gctcctgacg attccccaaa tcacgggaat
      901 agcgggaggc cccggtccct tccaagaggc tcccgtctca tgttagaata cctgatcccc
      961 tgccggcgga cttgataata acgaggtgcg gagtgatggc gtacatgacc ctttggctga
     1021 agaaggtatc aagacgagtt caggaattcc gtcttactcc ccgaatcctt aaagagtggg
     1081 cgctgatcca cttcggctgc ttccttttcg cggcgggagt gacctggttc ctggtgccga
     1141 ataatatggc ggccgggggt atcagcggtt tcgccatggt gatcaatcac ttcttcccga
     1201 atatgcccgt cggggttctc atgatggcca tgaacgtaat cctctttata atcgggttca
     1261 tattcatcgg gttcgagttc ggggccaaga ccatttattc gagcctcttc ctgtcgggtg
     1321 cggtctggtt cctcgaacgt tttttcccgg tctcgggtcc cctctccgac gacatcatga
     1381 tccagctgat ctttgggacc ctcctcgggg cgacgggcat ggcgatatta ttcaaccaaa
     1441 acgcctcgac ggggggcacc gacattaccg ccaagatcct gaacaagtat ctgaacatcg
     1501 atctgggcaa aggtgttctg ttatgcgact tttcctatgt gcttgccgcc gcctgggtct
     1561 ttggcatccg cataggcatg tacgccgcgc tg
//
LOCUS       DAOSRG010000277         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_72970_length_1585_cov_10.966013, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000277 DAOSRG010000000
VERSION     DAOSRG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72970_length_1585_cov_10.966013"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLM30_07000"
     CDS             <1..837
                     /locus_tag="PLM30_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOR54957.1"
                     /translation="VIEFQDAASSGAPARAITDMLVTELFNTNLFTIIERSRVDSILL
                     EHSLVLSGTIDPATAAQVGKLLGVEYLITGSITEYRHEAAGGVVPLPGLPGGVAVGSH
                     TAYVTIDTRAIDAQTGEIRMVAREQGASNQTLGGVAYSGAAFGGGKTGGVLAGATHKA
                     VLNLVERFRELAASTAARPVEFNVIQAEGSQVMVDGGSANSGLAPGAFLAAYREGALI
                     TAMDGTVLDAEKIYLSILSVVEVKPQYSICSIIRGIPLQRGDKAETLGDRSPESIALG
                     SR"
     gene            complement(845..1147)
                     /locus_tag="PLM30_07005"
     CDS             complement(845..1147)
                     /locus_tag="PLM30_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR54958.1"
                     /translation="MPEALPDGEVFEDLLAGPPVRIERILSSGQATPPGTWLDQDDDE
                     WVALLQGEATLEYQDSSRVSLRSGDWVFIPAHTGHRVASTSSDPFCLWLAVHGRLR"
     gene            1338..>1585
                     /locus_tag="PLM30_07010"
     CDS             1338..>1585
                     /locus_tag="PLM30_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54959.1"
                     /translation="MCRMLMANRSGISRLVERLSEFPDCNTLAAFLKGLDLRHGGHGG
                     GVVLYGDGSVLHYEKGVDMQPERAAAVLEGRDYEWAVF"
BASE COUNT          275 a          511 c          539 g          260 t
ORIGIN      
        1 gtcatagaat tccaggatgc cgcctcgtcg ggggctcccg cgcgggccat aacggacatg
       61 ctcgtcacgg aactcttcaa caccaacctc ttcaccatca tcgagaggtc gagggtggac
      121 tcgatccttc tcgagcacag ccttgtgctg tcgggcacga tcgacccggc caccgccgcc
      181 caggtcggca aactgctggg ggtcgagtac ctcatcaccg gttccatcac cgagtaccgg
      241 cacgaggcgg ccggcggggt ggtacccctc ccgggccttc ccggcggcgt ggccgtggga
      301 tcccacacgg cctacgtgac catcgatacc agggcgatcg acgcccagac cggggagatc
      361 cggatggtgg cccgtgaaca gggggcctcg aaccagaccc tgggcggagt cgcttatagc
      421 ggcgccgcct tcggcggggg caagacggga ggggtcctgg ccggcgcgac ccataaggcc
      481 gtgctgaacc tggtggaaag gttcagggaa ctcgccgctt ctacagcggc aaggcccgtg
      541 gagttcaacg tcatccaggc cgagggttcc caggtcatgg tggacggtgg aagcgccaat
      601 tcggggctgg cgccgggggc cttcctcgcc gcctacaggg agggcgccct gataaccgcc
      661 atggacggca cggtgctcga cgccgagaag atctaccttt ccatcctcag cgtggtggag
      721 gtcaagcccc agtacagcat ctgctccata atcaggggga tccccctcca gcggggcgac
      781 aaggcggaga ccctgggcga ccgctcgccc gagagcatcg ccctcggctc gaggtgaccg
      841 ggttttagcg cagtctcccg tggacggcca gccagaggca gaacgggtcc gatgaggtcg
      901 acgccacccg gtgcccggtg tgggccggta tgaagaccca gtcgccggag cggaggctca
      961 ccctggagga gtcttggtat tccagggtcg cctcgccctg caaaagcgcc acccactcgt
     1021 cgtcatcctg atccagccag gtgcctggcg gtgtcgcctg ccccgacgac aggatcctct
     1081 ctatcctcac cgggggtccc gccaggaggt cttcgaagac ctccccgtcg gggagcgcct
     1141 ccggcaagga aaaaagattg cccgaaggta ccatggtgac cgcctcccag cgtcaagggt
     1201 tttcggccaa gcctcccctg gaatatacac tatagggccc gtcgctgagg gggcgggggg
     1261 cgcttcaatt gtcggcgccg ctggtatatc atggccttca ggggccgcga agaccccgat
     1321 gggggaggcg agggatcatg tgcaggatgc tgatggcgaa caggtcgggc atatccaggc
     1381 tcgtcgagag gctttcggag ttccccgact gcaataccct ggccgccttc ctcaagggcc
     1441 tcgatctccg tcacgggggc cacgggggcg gtgtggttct ttacggcgac gggtcggtcc
     1501 tccattatga gaagggcgtg gacatgcagc ccgagcgcgc cgcggccgta ctggaggggc
     1561 gcgactacga gtgggccgtc ttcca
//
LOCUS       DAOSRG010000278         1565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_74314_length_1565_cov_9.111258, whole genome shotgun sequence.
ACCESSION   DAOSRG010000278 DAOSRG010000000
VERSION     DAOSRG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74314_length_1565_cov_9.111258"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1565)
                     /locus_tag="PLM30_07015"
     CDS             complement(<1..>1565)
                     /locus_tag="PLM30_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOR54960.1"
                     /translation="PPATGQDLLERVGAGCDRKIVAWHVNRYLRPLDWPLREDSVIDF
                     VDTSSFEGMSVYRDTLTFVLSLACRRALGEDIFVRHSISDGYYCELQSGPALEERTGL
                     VRRAVEQVIRRDLPIKSEILSIDAAERILHQQNEPDTADLLKYAGVDPVTLYRCDGVY
                     GFFHGPLAPSTGSLTSWDLVPFRDGMVLRFPTVAYPLVLPPFTPAEKLADVFNEYSGW
                     LQILGVRTMENLHGQVTEERAAELVLVSEALHSQKFNRMSERISTSRKVRLVRISGPS
                     GSGKTTTAMRLAIHLRAMGARPVVVSLDDYFLERDKTPRDSRGEHDFETIDAMDLDLI
                     NGNLEDLLDGREVTLPRFNFLTGRREEGPKVTMRSDEVLIIEGIHGLNSRISSSIPAE
                     FKFSVYVSPLTGISLDRHNRTSTTDNRLLRRLVRDRRLRGKSAGTTLAQWPSVIRGAH
                     RYIFPYQELADEMFNSALVFELSVLKGYAEPMLRTVREDSKQFGEAQRLLNILRFVPY
                     IPADFVPDNSILR"
BASE COUNT          252 a          466 c          554 g          293 t
ORIGIN      
        1 tcgcggagga tggagttgtc ggggacgaag tcggcgggga tgtagggtac gaaccggagg
       61 atgttgagca ggcgctgggc ctccccaaac tgcttcgagt cctccctgac ggtcctgagc
      121 atcggctcgg cgtaaccctt gaggacggag agttcgaaga ccagggccga gttgaacatc
      181 tcgtcggcca gttcctggta ggggaagatg taccggtggg cgccccggat gaccgacggc
      241 cactgcgcca gggtcgtgcc cgcgcttttt cccctgagcc gccggtccct cacgagcctt
      301 ctcaagagcc tgttgtcggt ggtgctggtc cggttatgcc tgtccaggct tatgcccgtg
      361 aggggcgaga cgtagacgct gaacttgaat tccgccggga tcgagctcga gatcctgctg
      421 ttgaggccgt ggatcccctc gatgatgagg acctcgtcgg acctcatcgt gaccttggga
      481 ccctcttccc tcctgcccgt gaggaagttg aaacggggca gggtgacctc cctcccgtcg
      541 agcaggtctt cgaggttgcc gtttatcagg tcgaggtcca tggcatcgat ggtctcgaag
      601 tcgtgttcgc cccgggagtc gcggggggtc ttgtcgcgct ccaggaagta gtcgtccagg
      661 gagacgacga cgggcctggc gcccatggcc ctgaggtgga tggccagcct catcgccgtg
      721 gtggtcttgc ccgaccccga ggggccggag atgcggacca gccttacttt ccgactcgta
      781 ctgatcctct ccgacatcct gttgaacttc tggctgtgaa gggcctcgct gaccagcacc
      841 agctcggcgg ccctctcctc ggtgacctgg ccgtggaggt tctccatggt ccggaccccc
      901 aggatctgca gccagccact gtactcgttg aagacatcgg cgagcttctc ggcgggggtg
      961 aagggcggca ggaccagggg gtaggcgacg gtgggaaacc ggagcaccat cccgtcgcgg
     1021 aaggggacca ggtcccagga ggtgaggctc cccgtggagg gcgccagggg gccgtggaag
     1081 aaaccgtaga cgccgtcgca ccggtaaagg gtcacggggt ccacgccggc gtacttgagg
     1141 aggtcggccg tatcgggctc gttctgttgg tgcagtatcc tctccgcggc gtcgatgctc
     1201 aggatctcgc tttttatagg gaggtccctg cggatgacct gctccacggc ccggcggacg
     1261 aggccggtcc gttcctccag ggccggcccg gactgcagtt cacagtagta gccgtcgctg
     1321 atggagtgcc ggacgaagat gtcctccccc agggcgcggc ggcacgccag ggagaggacg
     1381 aaggtcaggg tgtccctgta gacggacatc ccctcgaagg aggaggtgtc tacgaaatcg
     1441 atgacggagt cttcccgaag gggccagtcg aggggcctga ggtagcggtt gacgtgccag
     1501 gccacgattt tcctgtcgca accggcgccc accctctcga ggaggtcctg gccggtggcc
     1561 ggggg
//
LOCUS       DAOSRG010000279         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_74690_length_1560_cov_6.213953, whole genome shotgun sequence.
ACCESSION   DAOSRG010000279 DAOSRG010000000
VERSION     DAOSRG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74690_length_1560_cov_6.213953"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLM30_07020"
     CDS             complement(<1..265)
                     /locus_tag="PLM30_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54961.1"
                     /translation="MMRVLGVLFLSALLLSPCLAGPGAAAQAPGEVAAEKAEESPPTI
                     LGLTLTQGGSAMAVFQVTGQDLPVPMVINTDDHALTLLWEGTLL"
     gene            complement(262..855)
                     /locus_tag="PLM30_07025"
     CDS             complement(262..855)
                     /locus_tag="PLM30_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54962.1"
                     /translation="MAGPDSWLGTARELWKDIVEGDRFPRWLRVLLVLMLVGGILWSA
                     FLFRQMVITAQARDVPPPPSGGAAVEKEITSLQETAAGFRNAVLARSGSTQLAVLAAT
                     LARRPFSPSEPEAAEGVESGPASVSAPAPPEIWIKAVLIREKDAAAVVDIEGYGEGVI
                     LRRGTPFGGGLGKVLGISQEKVVVTWSGQQLDIPVDR"
     gene            complement(855..1373)
                     /locus_tag="PLM30_07030"
     CDS             complement(855..1373)
                     /locus_tag="PLM30_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54963.1"
                     /translation="MNLSLPRKRVVLSLFGVLLVLSVVVGQFLFARMVNEERRLIVEL
                     RTRQRVLEADIQDKSSLLQAYKRTNEEIGAYRVALPPDQVAFYSSVERELAKNGIQVN
                     SMKPSKPVAGTSSVQVDFLGPYYSVLGVFSDWRGMGSAVRMISVSLEADEPGHVKGTA
                     VLETALAAGGDQ"
     gene            complement(1370..>1560)
                     /locus_tag="PLM30_07035"
     CDS             complement(1370..>1560)
                     /locus_tag="PLM30_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54964.1"
                     /translation="SFGRALSEATVVSSVGFPVTTRVRQESGASVRFSLDCGIRDIMS
                     IAADASEEGSGHGEVASR"
BASE COUNT          257 a          543 c          518 g          242 t
ORIGIN      
        1 ggaggagcgt cccctcccag aggagggtca gggcgtgatc gtcggtgttg atgaccatcg
       61 ggacgggcag gtcctgcccg gtgacctgga agaccgccat ggcggagccg ccctgggtga
      121 gggtgagccc caggatcgtc gggggggact cttcagcctt ctcggcggcg acctccccgg
      181 gagcctgggc ggcggcgccg gggccggcta ggcacgggga gagcaggagg gccgacagga
      241 aaaggacccc caggaccctc atcaccggtc caccggtatg tctagttgct gaccggacca
      301 tgtgaccacc accttctctt gagaaatgcc aaggaccttt ccgaggcccc cgccgaaggg
      361 cgtccccctc ctgaggatga cgccctcgcc gtaaccttct atgtcgacca cagcggccgc
      421 gtccttttcc cttatcagga cagccttgat ccagatctcg ggtggcgccg gcgccgagac
      481 cgaagccggg ccactttcaa ccccctcggc cgcctccggt tccgacggcg agaagggcct
      541 gcgggccagc gtggcggcca ggacggccag ctgagtgctc cccgaccggg cgagcacggc
      601 gttcctgaag ccggcggccg tctcctggag ggaggttatc tccttttcca ccgctgcacc
      661 gcccgaaggc ggcgggggga cgtccctcgc ctgggcggtg atcaccatct gcctgaagag
      721 gaaggccgac cagaggatgc cgccgacgag catgaggacc agcagcaccc tgagccaccg
      781 ggggaagcgg tccccctcga cgatgtcctt ccacagttcg cgggccgtgc cgagccagct
      841 gtcgggtccg gccatcactg gtcacccccc gcggcgaggg ccgtctcgag gacggccgtg
      901 cccttcacgt gccccggctc gtcggcctcg aggctcacgc ttatcatcct gacggcgctt
      961 cccatgcccc tccagtcgga gaagaccccc agcaccgagt aatagggccc caggaagtcg
     1021 acctgcaccg aggaggtgcc ggcgacgggc ttggagggct tcatgctgtt gacctggatg
     1081 ccgttcttgg ccagttccct ctccacggag gagtaaaagg ccacctggtc gggggggagc
     1141 gccacccggt aggcgccgat ctcctcgttg gtcctcttgt aggcctgcag gaggctcgac
     1201 ttgtcctgga tgtccgcctc gaggaccctc tgccgggtcc gcagttccac gatgagcctc
     1261 cgctcctcgt tcaccatccg ggcgaagagg aactgcccca cgacgacgga aaggaccagc
     1321 aggacgccga agagggagag gaccaccctc ttgcgcggca gcgacaggtt catctcgagg
     1381 ccacctcccc gtgaccgctg ccttcttcgg aggcatcggc ggcgatggac atgatgtccc
     1441 tgatgccgca gtccagggag aacctcaccg aggccccgct ctcctggcgg accctcgtcg
     1501 tgacggggaa gccgacggaa ctcaccaccg tcgcctccga tagggccctg ccgaaggaga
//
LOCUS       DAOSRG010000280         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_76596_length_1534_cov_5.588235, whole genome shotgun sequence.
ACCESSION   DAOSRG010000280 DAOSRG010000000
VERSION     DAOSRG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76596_length_1534_cov_5.588235"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLM30_07040"
     CDS             <1..560
                     /locus_tag="PLM30_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOR54965.1"
                     /translation="LNLARDALAGCGISGARGIVNGTTNYILSRMEEGMGYGEALEKA
                     QELGYAEADPTGDVEGWDAAVKAQVLAMVVLGEPLRLEEVERVGITGITPEDIASARA
                     KGARIKLIAEVRRDGGRLAARVTPEEVPLTHPLAGVMGAANALTFYTDHLGEVTIVGP
                     GAGGVETGQALLTDLLALHRRHGKA"
     gene            complement(557..1459)
                     /locus_tag="PLM30_07045"
     CDS             complement(557..1459)
                     /locus_tag="PLM30_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR54966.1"
                     /translation="MSRPKTVHGLRFTIHDSRLTTYDSRYFAMRVTMSDVARAAQVNK
                     ATVSRVLRGDARISPATVEKVWAAIKELGYRPDAIARGLSSSRSDTVGVFLEDISLPW
                     AAEYLAGLERVLSRHGVDIILRATGGDPQQRYNALSGLLSRRVDGFILFDNGPAPEAD
                     IPSVIVGPKREGGHTVSVDLGSAASQLARLADGRAVELFQGENPFFPGIGALQPGGAP
                     RKEGTVRLFDGRLPQEEGTSPDDTVVISATRRCPAVPPGAWKLYWPAFELGTLSARLL
                     LNIIQGKGVRPQVVSVLPALSGPL"
BASE COUNT          255 a          481 c          569 g          229 t
ORIGIN      
        1 cgctgaacct ggcccgcgac gccctggccg gctgcggcat cagcggcgcc agggggatcg
       61 tcaacggtac caccaactac atcctttccc gaatggaaga gggcatgggc tacggcgagg
      121 ccctcgaaaa ggcccaggag ctgggctacg ccgaggcgga ccccacgggg gacgtggagg
      181 gctgggacgc cgccgtcaag gcccaggtgc tcgccatggt cgtcctgggg gagccgctgc
      241 gcctcgagga ggtggagcgc gtgggcataa cgggcattac gcccgaggat atcgcctcag
      301 cccgggcgaa gggcgccagg atcaagctta tcgccgaggt gagaagggac ggcgggaggc
      361 tggccgcccg ggtcacgccc gaggaggtgc ccttgactca cccgctggcg ggcgtcatgg
      421 gcgccgccaa cgcccttacc ttttacacgg accacctggg ggaggtcacc atcgtcgggc
      481 ccggcgcggg gggcgtggag acggggcagg ccctgctcac agacctgctc gcccttcacc
      541 ggcggcacgg gaaagcctag aggggacccg aaagtgccgg gaggaccgac accacctggg
      601 ggcggacgcc cttgccctgg atgatattga gcagcagccg ggccgaaagg gtgcccagtt
      661 cgaaggcggg ccagtacagt ttccaagccc ctggggggac cgcggggcag cgccgggtgg
      721 cgctgatgac gacggtatca tcgggcgagg tcccctcttc ttgagggagg cggccgtcga
      781 agagcctgac ggtcccttct ttcctgggcg cgccgccggg ctggagggca ccgatgccgg
      841 ggaagaaggg gttctccccc tggaagagtt cgaccgcacg gccgtcggca agccgggcca
      901 gctgcgaagc ggcggagccc aggtccaccg agaccgtgtg gccgccctct cgcttggggc
      961 ccacgatgac ggaggggatg tccgcctccg gcgccgggcc gttgtcgaag aggatgaagc
     1021 cgtcgaccct ccttgaaagg agccccgaca gggcgttgta cctctgctgg gggtctcccc
     1081 ccgtcgcgcg gaggatgata tcgacgccgt ggcgggagag gaccctttcg aggcccgcca
     1141 ggtactccgc cgcccagggc agcgagatat cctccaggaa gacccctacg gtgtcgctcc
     1201 tgctgcttga aaggcccctg gcgatggcgt cggggcggta gcccagttcc tttatggcgg
     1261 cccagacttt ctcgaccgtg gcggggctta tgcgagcgtc gccccggagg accctgctga
     1321 cggtggcctt gttgacctgg gcggccctgg cgacgtcgga catggtcacg cgcatggcga
     1381 agtaccgtga atcgtaagtc gtgagtcgtg agtcgtgaat cgtgaaccgc aaaccgtgga
     1441 cggtttttgg tctactcaaa gcacccgccc ccgatgaact cgcggaggat ggagttgtcg
     1501 gggacgaagt cggcggggat gtagggtacg aacc
//
LOCUS       DAOSRG010000281         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_78027_length_1515_cov_9.498630, whole genome shotgun sequence.
ACCESSION   DAOSRG010000281 DAOSRG010000000
VERSION     DAOSRG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78027_length_1515_cov_9.498630"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLM30_07050"
     CDS             <1..258
                     /locus_tag="PLM30_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR54967.1"
                     /translation="AFNIFSGHVGRVELLTGYEGNAFASTGDPEADILDITAVHPMRR
                     DAVEELLARSGAEWSLVEGMLRDGKITMAPWEGEEFFIRSF"
     gene            598..1464
                     /locus_tag="PLM30_07055"
     CDS             598..1464
                     /locus_tag="PLM30_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR54968.1"
                     /translation="MELDKILSKENFLFSGKSKDIYLIPKGRYKGKYALVFTDRATGY
                     LDKDGKAVFDPGYDDVVGEIPGKGAIACKFAAYFFKLLAGQGVPTHFIDTVAEDVMVV
                     EPAVPIGMPEQGPEFPGSAPLLNLEWTWRHSAMGSFWRRYPFVRPCADLPVVVEAWTK
                     GKVDRLITFESLVGAGVMTAEEVAFAEGFVKDIARVIYDEFAARGLHVLDGKVELGRL
                     KEGDGRIVLIDEISPDVLRVCNGYLEGKRGCLRANECITTSLSGEERRISAGYQLVAS
                     ELKKIFLKEVRK"
BASE COUNT          317 a          421 c          467 g          310 t
ORIGIN      
        1 gccttcaata tcttctcggg gcatgtgggg cgagtggaac tgctcacggg gtacgagggc
       61 aacgccttcg cctcgacggg tgaccccgag gccgatatcc ttgacataac ggccgtccac
      121 cctatgagga gggacgctgt ggaggagctc ctggccagga gcggggccga gtggagcctg
      181 gtggagggaa tgctccggga cgggaaaata acgatggccc cctgggaagg ggaggagttc
      241 ttcattcgct ccttctgagc ggcggggtca ggtctacact tttgactttt ccgcactttt
      301 ggcaactttc tgacgttttc aggtttaacc ctcctgccca ccccagccag gatcccttac
      361 ctggcctggg ttccttcgga gtgccccttt cattccaggc ttgatctctt atcctggaac
      421 tccgttactt atccaaaaga actaactcaa aaggatttag tcaaaagtgt agacctgacc
      481 ccgaatctgc cgaacttggg atttagtcaa aagtgtagac ctgaccccgc tcttggattt
      541 tcaattctgc tccgggctgg taagatgact ttcgatacct accgtggagg tcgaacgatg
      601 gaactggata aaatcctatc gaaagagaac ttccttttct ccggaaaatc caaggatatc
      661 tacctaatcc ccaaggggag gtacaagggc aagtacgcct tggtcttcac cgaccgcgcc
      721 accggctact tggacaagga cggaaaggcc gtcttcgacc ccggctacga cgacgtcgtc
      781 ggcgagatcc cgggcaaggg ggccatagcc tgcaaattcg ccgcctactt tttcaagctc
      841 ctggccgggc agggggtgcc gacccatttc atcgacaccg tggccgagga cgtcatggtg
      901 gtggagcccg ccgtgccgat aggcatgcca gagcaggggc cggagttccc gggctcggcg
      961 ccgctcctca acctggagtg gacctggcgc cacagcgcca tggggagctt ctggcggcgc
     1021 taccccttcg tcaggccctg cgccgaccta cccgtggtcg tcgaggcatg gaccaagggg
     1081 aaggttgaca ggctgataac cttcgaatcg ctggtgggcg ccggcgtgat gacggcggag
     1141 gaagtcgctt tcgccgaggg cttcgtaaag gatatcgccc gggtcatcta cgacgagttc
     1201 gccgcccggg ggctgcacgt cctcgacggg aaggtcgagc ttgggcgcct caaggaaggc
     1261 gacggtcgca tagtgctgat cgacgagata tcgcccgacg tcctgcgggt ctgcaacggc
     1321 tacctggagg ggaaaagggg ctgcctcagg gccaatgagt gcatcaccac ttccctttct
     1381 ggtgaagagc gcagaatatc cgccgggtat caacttgtcg cgtcggaatt gaagaaaata
     1441 ttcctgaagg aggtaagaaa atgatctcgg tgcgtgaaac ggccatagga tggatagcca
     1501 ttgaggagaa ggacg
//
LOCUS       DAOSRG010000282         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_78032_length_1515_cov_5.586301, whole genome shotgun sequence.
ACCESSION   DAOSRG010000282 DAOSRG010000000
VERSION     DAOSRG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78032_length_1515_cov_5.586301"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLM30_07060"
     CDS             complement(<1..214)
                     /locus_tag="PLM30_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54969.1"
                     /translation="MPGTFGWIALFAFLAAFVNALGILMVFRYRERAEKIMAYLMCFA
                     AGVLITTPLMLALPEAVRRYPHAGFLA"
     gene            450..>1515
                     /locus_tag="PLM30_07065"
     CDS             450..>1515
                     /locus_tag="PLM30_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOR54970.1"
                     /translation="MMAFTGKKERGSGNAIPEREDLDRAYQWGLEDLFEDRAQWERAY
                     EETGPLMDSLEPFSGTLASSPSRLLECLKLRDRLGELTSRVFAYAHMKSHEDTRDPDG
                     QAIAARATTLAAAAARSGAFITPEILSIPVETLSLFLEREEGLGLYRFFLEELQRKRA
                     HVLSQEEEGLLASSTELAHAPENIFSMLTNADMTFPPIRDELGHETPFSEERFGSFLQ
                     SRDRRVREDAYKNLYGVYGRYRNTLSASLNAALRVSLFYSRARGFGSNLEAALHEDDI
                     PLSVYDSLVEGVREGLDALHRYVALRGRVLGVSPVRMFDLYVPLVDEYGGEIPYPEAM
                     DMVREGLAPLGDDYLAALDEG"
BASE COUNT          282 a          484 c          460 g          289 t
ORIGIN      
        1 gggccaggaa acccgcgtgg ggatacctgc ggacggcctc gggcaacgcc agcatgagcg
       61 gcgtcgttat caggacgccc gccgcgaagc acatcaggta cgccattatt ttctccgcac
      121 gctcgcggta cctgaagacc atcagtatcc ccagggcgtt cacgaaggcg gccaggaaag
      181 cgaaaagggc tatccagcca aaggttcccg gcaatatcta ccctccccaa tgacccggtc
      241 cgaccgcttc ttattgtaga tcctattccc ccgcggggga acaaaaactc tcaccttgtg
      301 acattgctct cacgggctga gcccgtcggc ctccaccatc tcctcccggg gcgttttttc
      361 ccttcttgtg ctagcatcgg aaggtcttga acatcctggg gtggggaagt cctttccccg
      421 ccggggcggg gttttgagga ggccggtcga tgatggcttt tacggggaaa aaggagcggg
      481 ggtcgggcaa tgccattccc gagagggaag acctggatag ggcctaccag tggggcctgg
      541 aagacctctt cgaggacagg gcgcagtggg aaagggccta tgaagagacc ggccctctca
      601 tggactccct tgagcccttc agcggcaccc tggcatcctc gccgtcgaga ttgctggagt
      661 gcctcaaact gcgtgacaga cttggcgaac tgacctccag ggtattcgcc tacgcccaca
      721 tgaaaagcca cgaggacacc agggaccccg acggccaggc catcgccgcg agggcgacca
      781 ccctggccgc ggctgcggcc cgttcagggg ccttcatcac cccggagatc ttgtcgatac
      841 cggtagagac cctctccctt ttcctggaga gggaggaagg gctcgggctt taccgtttct
      901 tcctggagga gttacagcgc aagcgcgccc acgtactgtc ccaggaggaa gaagggctac
      961 tggcctcctc gacggaactc gcccacgccc cggagaatat cttttccatg ctcaccaacg
     1021 ccgacatgac ctttccgccc atccgcgacg aactgggtca tgagacgccc ttctcggagg
     1081 aacggttcgg ctcttttctg cagagccgtg accggagggt cagggaggac gcctacaaaa
     1141 atctttacgg cgtctacggt cggtacagga acaccctctc ggcttcactg aacgctgcgc
     1201 tgcgggtttc gctcttctac tcgcgggcgc ggggcttcgg gtcgaacctc gaagcggctc
     1261 ttcacgagga cgatatcccc ctctccgttt acgactccct cgtggaaggc gtccgggagg
     1321 ggcttgacgc gctccatcgc tacgtggccc tccgagggag ggtcctaggc gtttcccccg
     1381 tcaggatgtt cgacctttac gtccccctgg tcgacgagta cggcggcgag ataccctacc
     1441 cggaagccat ggatatggtt cgcgaggggc tcgcccccct gggggatgac tacctggccg
     1501 ccctcgatga gggct
//
LOCUS       DAOSRG010000283         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_78230_length_1512_cov_14.131091, whole genome shotgun
            sequence.
ACCESSION   DAOSRG010000283 DAOSRG010000000
VERSION     DAOSRG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78230_length_1512_cov_14.131091"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..511)
                     /locus_tag="PLM30_07070"
     CDS             complement(125..511)
                     /locus_tag="PLM30_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR54971.1"
                     /translation="MAVTPYDLQELYRFTRWGRSRSIEQIEKMLQGTTMCFSVRYASK
                     MVAFCRVLSDLVFRASLWDIMVHPDHQGKGLGSSLLDYALDHPKLRDIPLVITYTSEL
                     APFLARLGFRHEEGAMMLLRKPIEYS"
     gene            complement(550..777)
                     /locus_tag="PLM30_07075"
     CDS             complement(550..777)
                     /locus_tag="PLM30_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOR54972.1"
                     /translation="MLIVACQVCGEMRILPGTPDADGVARTVWTCPCCGAGQVLQLPV
                     STDASGGELRRIVAGMGFSPDAPLEEEKNGL"
     gene            complement(812..>1512)
                     /locus_tag="PLM30_07080"
     CDS             complement(812..>1512)
                     /locus_tag="PLM30_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOR54973.1"
                     /translation="QERDRLDLEVNALRAESERLREGLEYVREGRIIIFAGEMIAQTV
                     VVTRPGEPRPSPEEVEETLMKSARANIAMRSGTDPEQVEITLDPHSEEMIGECCSGTP
                     GRMILRLIVSENTVQGEPVKGSITLHESRKIYDKGYTLAEVGDIPAGLGQEEAEMRLF
                     AILRGVNSKAQRDGVLPDPLKGTVGNLSASKFFEAVDLLTLREKTSRVIVKAEDDIYT
                     EGPVRVEILVIDEG"
BASE COUNT          279 a          498 c          415 g          320 t
ORIGIN      
        1 tccgtgaccg attcggatga aacaagatac cttctctcca attcctttcc acctcccctt
       61 cttaatggcc gtgccccgcc tggctcaagg gagccggggc gcggcttaat ggcagtggga
      121 ccgctcagga atactcgatc ggtttccgga ggagcatcat cgccccttct tcatgcctga
      181 aaccaagcct ggccaggaag ggagcgagtt cgctcgtata ggtgatgacc agggggatat
      241 ccctcaactt ggggtgatcg agggcgtaat ccagcaatga cgaccccagc ccctttccct
      301 ggtgatcggg gtggaccatt atgtcccaga gggaagccct gaaaactaga tccgacagga
      361 cacggcagaa ggccaccatt ttggaagcgt acctgaccga aaagcacatg gtggtcccct
      421 gcagcatctt ctctatctgc tcgatggacc ggctcctgcc ccaccgggtg aaccggtaaa
      481 gttcctggag gtcgtagggc gtgacggcca tcttgctgtc gtagtagagg taacgttcct
      541 cgacgtcctt caaagtccgt ttttctcctc ttccaggggg gcgtcgggtg aaaaacccat
      601 accggcgacg attcgccgga gctccccgcc gctggcatcg gtgctgaccg gcagctggag
      661 gacctggcca gccccgcaac aggggcatgt ccagacggtc ctcgccacgc cgtcggcgtc
      721 gggcgtaccg ggcaatatcc tcatttcccc gcagacctgg caagcaacga taagcattta
      781 cccaccccct tcatcccaca gaacaaacgt gtcatccttc atcgatgacc aggatctcca
      841 cacgaacagg cccttcagta taaatgtcat cctccgcctt gacaatgacc ctggacgttt
      901 tttccctcag ggtcaacagg tccacggctt cgaagaactt ggaggcgctg aggttgccca
      961 cggtcccctt cagggggtcg gggagcacac cgtccctctg ggccttcgaa ttgacccccc
     1021 tgaggatggc gaagaggcgc atctccgcct cctcctgccc aaggccggcc ggtatgtctc
     1081 ccacctcggc cagggtgtat cctttgtcat agatcttcct gctctcgtgc agggtgatgg
     1141 aacccttcac cggttccccc tggacggtat tctccgacac gatgagcctg aggatcatcc
     1201 tccccggggt tcccgagcag cactccccta tcatttcctc ggagtgaggg tccagggtga
     1261 tctccacctg ctccgggtcc gtcccggagc gcatggcgat gttggccctg gccgacttca
     1321 tgagtgtttc ctcgacctct tccggtgaag ggcggggctc cccgggtcgt gtcacgacga
     1381 cggtctgggc gatcatctcc ccggcgaaga ttatgatacg cccttcccgg acgtactcaa
     1441 gaccctccct gagcctctcc gattcggccc tgagggcgtt cacctccagg tcgagccgat
     1501 ccctctcctg ct
//
LOCUS       DAOSRG010000284         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2273
            NODE_78775_length_1505_cov_9.471724, whole genome shotgun sequence.
ACCESSION   DAOSRG010000284 DAOSRG010000000
VERSION     DAOSRG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864340
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:51:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,418
            CDSs (total)                      :: 1,395
            Genes (coding)                    :: 1,394
            CDSs (with protein)               :: 1,394
            Genes (RNA)                       :: 23
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78775_length_1505_cov_9.471724"
                     /isolate="AMR_MDS_2273"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLM30_07085"
     CDS             <1..1205
                     /locus_tag="PLM30_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR54974.1"
                     /translation="RLYARGPNSAIFYTMGITQHQYGTNNVKTVADLALLCGMLGRPG
                     TGVNPLRGQNNVQGACDMGCLPATLPGYLDIAKNTEKAVEKVRQIWKAELPSSPGKTI
                     VKVTDGLAKGEIKGLFIMGENPMVSDPNTNHVKNALEKAEFLVVQDIFMTETAQLADV
                     VLPAACWAEKDGTFTNTCRAVQRIRKAVDAPGDARPDWEIIVDLAKACGADWDFHSPE
                     DVMEEISRFVPQYGGITYDRLDQGPLMWPCPDKDHPGTPNLYTTGFPRGKATFVPYEW
                     NQPHEWPDAEYPFLATTGRTLYHYHTGSMTRRTPSAEYIKSLYVEVNPEDAAAIGLKE
                     GDDVRVTSRRGSLVGETRITDRVSPSMVFVPFHFGEQPANVLTASVWDDTSETPAYKI
                     NAVKVEKA"
     gene            complement(1294..>1505)
                     /locus_tag="PLM30_07090"
     CDS             complement(1294..>1505)
                     /locus_tag="PLM30_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR54975.1"
                     /translation="KILGIDSRGRLQVVKAQVPLAEMFRYAIILRSMTSGRGNFTMEL
                     SHYEEVPHDIAKKIIAEAEVQEAEE"
BASE COUNT          327 a          468 c          441 g          269 t
ORIGIN      
        1 cccggctcta tgcccgtggc cctaactcgg cgatcttcta caccatgggc atcacacagc
       61 accagtacgg caccaataat gtgaaaaccg tggccgatct cgctttgctc tgcggtatgc
      121 tcggaaggcc cggcacgggc gtaaacccct tgcgtggcca gaacaatgtc cagggcgcct
      181 gtgacatggg ttgcctgccg gcgacgctcc cgggttacct cgacatcgcc aagaacaccg
      241 aaaaagccgt tgagaaggta aggcagatct ggaaggctga actgccctct tcgccgggca
      301 agaccatcgt caaggtcacc gacgggctcg ccaagggcga gatcaaggga ctgttcatca
      361 tgggggagaa ccccatggtc agcgatccca acaccaacca cgtcaagaac gctctcgaaa
      421 aggcggaatt tctcgttgtc caggatattt tcatgaccga gacggcccag ctggccgacg
      481 tggtactccc ggcggcatgc tgggcggaaa aggacggcac cttcacgaat acctgcaggg
      541 ccgtgcagag gatccgtaag gccgttgacg cccccggcga cgcccgcccg gattgggaga
      601 tcatcgttga cctggccaaa gcctgcgggg cggactggga cttccattcc cccgaggacg
      661 taatggagga gataagcagg tttgtgccgc agtacggcgg tatcacctac gaccgcctgg
      721 accagggccc cctcatgtgg ccctgccccg acaaggatca ccccgggaca ccgaaccttt
      781 acaccacggg tttccccagg gggaaggcga ccttcgtgcc ctacgaatgg aaccagcccc
      841 acgaatggcc cgatgccgag taccccttcc tggctaccac gggaaggacg ctctaccact
      901 atcacacggg ctcgatgacc aggaggaccc cctcggctga gtacatcaag tcgctctacg
      961 tggaggtcaa ccccgaggat gcggccgcga tcgggttaaa agaaggggat gacgtgaggg
     1021 tgacatcgag gcgcgggagc ttggtgggtg aaacacggat caccgacagg gtctcaccca
     1081 gcatggtgtt cgtacccttc cacttcggcg aacaacccgc caacgtcctg acggcctcgg
     1141 tctgggacga tacttcggag acgccggcgt acaagatcaa cgccgtcaag gtcgagaaag
     1201 cctgaaatta gccccaggac ctacgatcaa aaaggggcgc ggggaaagac cccgcgcccc
     1261 tttttcttta ggcctttaat agggccggct attctattcc tcggcctcct ggacctcggc
     1321 ctcggcaatg atcttcttgg cgatatcgtg gggcacttcc tcgtaatggg agagctccat
     1381 ggtgaagtta cctcgcccgg aggtcatgga cctgaggata atcgcatagc ggaacatctc
     1441 tgccagtggt acctgggctt tcacgacctg gagccttccc cggctgtcga tccccaaaat
     1501 cttgc
//
