LOCUS       DAOSRI010000001        20151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_2849_length_20151_cov_3.890476, whole genome shotgun sequence.
ACCESSION   DAOSRI010000001 DAOSRI010000000
VERSION     DAOSRI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 20151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20151
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_20151_cov_3.890476"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /gene="mgtA"
                     /locus_tag="PK717_00005"
     CDS             <1..1399
                     /gene="mgtA"
                     /locus_tag="PK717_00005"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOR46089.1"
                     /translation="HVKENFKKVDEIPFDFVRRRMSVVVEDASGKQIIICKGAVEEIF
                     SVCTQIQTQGVVEKLGQEHMQKAKELVEWSNSNGFRALGLAYREMPKRQEAYCVSDEQ
                     EMTLLGFLTFLDPPKVTAKQAIKDLRDLGVSVKILTGDNDLVSKKICSQVGLETGSIV
                     LGSQLEEMDESQLVEIVEKTNVFAKLAPSHKERIITILKKKGHVVGFMGDGINDAPAL
                     KAADVGISVDTAVDIAKESSDIILLGESLTVLQDGLIEGRRVFGNIIKYIKMAASSNF
                     GNMFSILGASFFIPFLPLLPIQILTNNLLYDFSQTAIPTDKVDEEWIQKPRKWTIGSI
                     RQFIIFIGPISSIFDYLTFFVMIFIFGGLGKPELFRTGWFVESLLTQTLIIHVIRTNK
                     VPFLQSRASLPLTITSIVIALTGIYLPYSALAGTLGLVGLPPLYWPIVFAMLVCYVVL
                     TQVVKTWFIKKYPEK"
     gene            1490..2101
                     /locus_tag="PK717_00010"
     CDS             1490..2101
                     /locus_tag="PK717_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOR46090.1"
                     /translation="MEKIDLKKQLAGLYNPPKTPVMVEVPQFNFIMVDGKGDPNTSEE
                     YQNAMQVLYSLSYTLKFMAKKVLEKDYTVMVLEGLWWAENMDDFLEGNKSNWLWTAMI
                     MQPDFITREHYEATLSEVKKKKPNPSLGKARFEAFREGPSAQIMYIGPYSAEGPTIAA
                     LHEFIDRGGMKPRGKHHEIYLGDPRKADPAKLRTAIRQPVEKA"
     gene            complement(3780..4055)
                     /locus_tag="PK717_00015"
     CDS             complement(3780..4055)
                     /locus_tag="PK717_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46091.1"
                     /translation="MKIKPYIAGILFVVLFIGLIQLAQGVGFWSSREHGEGRGRPPEG
                     FQNGQEFQMPPGEEGEENEEGEGNQYRYRIRNEEGETEEGEGLEGQP"
     gene            complement(4061..4843)
                     /locus_tag="PK717_00020"
     CDS             complement(4061..4843)
                     /locus_tag="PK717_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46092.1"
                     /translation="MKQKRYALIRHLSQIGFVLLILYYTLGRFIFPGVRGIELHGICP
                     FGGVETLPTFLASGGLKFVHETGLSNFAMLAALLLAVLAFGGAFCGWVCPVGSVTEWL
                     FMLRRVFWKKRIIVPEKLHNVLVWFRYVVLAAILYMTFSIGFLVFGTVDPFLNLFSFG
                     RVFAVGLVVLVLILIGSMFIERFFCRYLCPLGAIILPFAKLSATGVVHSNEDCTSCKG
                     CEEVCPKQIPISRQTKIDRGQCISCMKCVEECPTALSFKIGW"
     gene            5127..5315
                     /locus_tag="PK717_00025"
     CDS             5127..5315
                     /locus_tag="PK717_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46093.1"
                     /translation="MPYQHNINSIKVSVESGLVNTEIMEPISGLSVVWIDQQNYNPAT
                     MEVCSCPNYRCWTNNAMV"
     gene            5458..6729
                     /locus_tag="PK717_00030"
     CDS             5458..6729
                     /locus_tag="PK717_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOR46094.1"
                     /translation="MSTISGSIIIIDKRLISLINSGNLPEEEKTCKELSNAFFLVDEN
                     FDEKSFLIEKFSRETWKNDTLDITIAPSFACNFACPYCYEARDDRIMDEGTLKNLVRF
                     IQGHLKKFNIGHFIMACYGGEPLLFPDHFRYIYEEISKTNPSVKISPRMTTNGSLLRK
                     KLGGFMKSVPLERIQVTIHGDKKNHDSKRFYVGGKPSYEDVVNGIGEAINRKIPNIEI
                     RVNIDLENAFSLKGLLMDLDKRGFSNHENLRVYAYPVVHMTPESLGYKSKCLSIDSYF
                     EKITGIIELINAYGFCGGAKHIRKYIKNLVLSNLCGVGTKGFYTVSPDGAIGTCWEFI
                     GREGFEIGNVNFSAPIDCMKEKMWIDARISKVKRCLDLECPLVPSCAGGCVAQSFSER
                     GDLGEPACFKKPEQIAGYLKFYADMSMRYSI"
     gene            complement(6746..7252)
                     /locus_tag="PK717_00035"
     CDS             complement(6746..7252)
                     /locus_tag="PK717_00035"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOR46095.1"
                     /translation="MESGNQNSSDNISGKTMRPDKPTYFMMITDIVAKRSTCLRRQVG
                     CVLVDSDGNIKATGYNGVVAGMTHCDTCQRELLASGSDLWQCPVIHAEINAISRYEGP
                     RKDITQIFCTTLPCFHCAKVIANTFPNLEELHYHDDYPHSELIKEVFINKGIKLMLHK
                     DGEVEEAR"
     gene            complement(7201..8850)
                     /locus_tag="PK717_00040"
     CDS             complement(7201..8850)
                     /locus_tag="PK717_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46096.1"
                     /translation="MKKLTAFLLCALVIMPCFQTALSQDDKLIELSPKEINIVAKQGE
                     TATATMGIFNRGSSSLLFSLSKSYSKAKTQPLASPDGNMLVYPSFLDFGTVPSKGEVS
                     QTVTCTAPTEQYLQVTASSDVEWIKCLIRNIDKTVVSVTVTVKLDGMVTGQLYRGNVI
                     LASNAGLVQVPVILQVATTTVKDWLSYNPTNGIVSPGNNTGIQIKASAKGLPPGDQFA
                     TIIVTSNDEKQPVIEVPVKLTVIAGSPAPSTPEIFVGYPGDGRAHLFWKPIASPNIVG
                     YYIFRSNNPGIYTDVAITDFYVTGTQYTDPNVTNGLIYYYIIKSVDQNGNLSDASNEI
                     MVMPNPIKPVVNLKDGLVTRTQVLDVTGVAEPNSQIIVNGELAIIDPDGKFQAKTVLR
                     VGSNTITVIVFDPGGNQTKFTYKVSFSAYTTVILMVGSKNAWVNGEEKKNALPQAPVI
                     ISGKTFVPFRFLGESLGADIGWDDQTKKVTYTLEGKKVEMWIGKTTASVDGKPVSVNP
                     PPQVIGGSTFVPTRFITEALGASIEWKAATKTVVITYPAKP"
     gene            complement(8912..9682)
                     /gene="murI"
                     /locus_tag="PK717_00045"
     CDS             complement(8912..9682)
                     /gene="murI"
                     /locus_tag="PK717_00045"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOR46097.1"
                     /translation="MIGFFDSGVGGLSILKNALEILPNHDYAYFGDEAYCPWGEKPKE
                     FIIERSRLITIKLISLGTKAIVVACNTATVNAISTLRAEFPNIPFIGVEPGVKPATTL
                     TESGKIAVLATKATSLGERFLQLVNLFAQDKDIIMIPSSGLVELVEQGKCEGEEVEER
                     LSEILLPYMKTGFDQLVLGCTHYPFLAGALRKILPSKVNIIDTGPPVAKQLARVIMQN
                     NILEGNGRVSLWTSGDPGKFCELASKLLGIEINKIERF"
     gene            complement(9679..10803)
                     /locus_tag="PK717_00050"
     CDS             complement(9679..10803)
                     /locus_tag="PK717_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOR46098.1"
                     /translation="MVKITKAQIIRYMLYAGALSIIAVLFVMQFATGLDFGSALKSLG
                     KTNLWFILAAIAINVSAYFTEALQLYLLAKTLGKKPSFWGMMLIFFIGNFFSNVTPAT
                     SGGKPFQIYFLLDQGFSIAEGTVMVIARGIISVSTRIFLVLAVAVAVLFGFDSNLSSF
                     IGWTFVLTLIMMIALIAMGIAALLNPKFFSFLINIASRIRLVRKLVKAPTVDECMAKG
                     YRFLDSVKNSSKMIFGGNKVYLTLTIFTSLVSWFLLKTVPYFVLLSFGESIPLMIVVA
                     TSIISQLLTIWVPTPGASGGVEIGLGTLFRGLVANPGNLSPFVLVYRLIDYHLNMLVG
                     LPIAFWALGKKVKPVICTPQNENEVETSAGVPECEGGTLK"
     gene            complement(10876..12033)
                     /locus_tag="PK717_00055"
     CDS             complement(10876..12033)
                     /locus_tag="PK717_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOR46099.1"
                     /translation="MAKMTAKTLTRYIVVAGLMSFVIIFIIMQFSTGFDFSKMGEAVS
                     KANWWWILAAALCNVASFLCEAFQLWFLSRALGKKPGFWRMMQIYFVGNFFSNATPST
                     SGGEPFQVYFMMDDGFSVAEGMVMVAVRGLISVMVRILYVLVTVALIALGIFRLNLSF
                     TFDLIFYITLAAFALLVVAGFIIIMNPHIFSFLVKFFSRFRWIRKLAKIQDPKQFIQK
                     GELFLGEIRSSAKLLIQGKKIWMIGAIFNSILTWVLLKTMPFFIMLALNEHPNIFVIF
                     AIGVISQLATAWVPTPGAVGGIETGMLAFASTMQTPGNIGLFVFIYRLMDYHMDVLVG
                     APIALRLLSKKLGKDTSNADLSALTSQISQKMEEEKEKMAQEELASEHKVN"
     gene            complement(12052..13086)
                     /locus_tag="PK717_00060"
     CDS             complement(12052..13086)
                     /locus_tag="PK717_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOR46100.1"
                     /translation="MAKKSSLLKKATKRVFVWLGIVVIPIFILASAIYVAEGGLPGSS
                     IKNFLDAVWWTVVTMSTVGYGDVVPITSIGRVIAILAMFSGIIMIAGLTATLASTLIE
                     RVVKRMNQNKVERFRDHVLILGWNENGLRIIGDLYRECQEKQIGIVVMADIEPPSSIQ
                     QDTYFIKGDPTRLENLDRASAEHAKAAVVLADFEINARNTDSKVILTALAVKRVSKNR
                     CRVICEVLLEEDAEYLRHAGVDEIIVRSGVAGDLLSRSVSNPGVAELMQILLKTESPG
                     SLGRISIPAPFHGKRYEELVMALYSQSEMIVVALIRSGNMMVNPSQNEIIQPGDEAFI
                     VSASGGECRI"
     gene            complement(13070..14071)
                     /locus_tag="PK717_00065"
     CDS             complement(13070..14071)
                     /locus_tag="PK717_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOR46101.1"
                     /translation="MGIFLEIIEWLDNSGVELLHREPPQGSADIKMGAQLIVREGQLA
                     IFMSNGKVADMFGPGKYTLSTENIPILTQLLSIPYGFKSPFRAEVLFISTVQMPGFKF
                     GTPDSIPFRDKELGVVRVKAFGNYTLRVDDPRKFITEIVGTKGFMTRGKIEDFMRGIV
                     ASKFTDMLGESLTTVFDLPKIYQEIGDQLSKSINGEVSKFGLSCLAAFIESIGLPPEV
                     ETAIDERAGMGVIKDMNAYLAYKAAKGMEKGGEGMMGAATQIGAGFVMGGKIVENLSK
                     GTDAKVSNEICPECGKPIQQNTKFCPNCGKNLQLNCKKCGQSVPEGAKFCPNCGEKI"
     gene            complement(14055..15134)
                     /gene="lysS"
                     /locus_tag="PK717_00070"
     CDS             complement(14055..15134)
                     /gene="lysS"
                     /locus_tag="PK717_00070"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOR46102.1"
                     /translation="MEGLPEKWHGLKDSEIRYRKRHLDMIANSEVRERFSKRTRIIRA
                     IREFLWNEGFEEVETPTLQPLYGGALAKPFMTHHNALDMDLYLRIAPELYLKRLIVGG
                     MPKVFEIGKAFRNEGISLVHNPEFTIMELYQAWADYHDMMDITERMVSSVAKTITGSF
                     VINFQGEEIDLTPPWKRLTMAEAFKEYAGVEIEKLRDAKFAFAFLQENGVKMEKKPGF
                     GGFCDETLKKLVEPKIKNPTFIYNYPIELSPLAKLIPGETMWVERFQPVIAGYEVGNA
                     FSELNDPQDQLNRFEGQEKARSSGDEEAHQTDTDFVETLAVGMPPTGGLGIGIDRIVM
                     LLTDQVSIREVLAFPQLRRQSGDIS"
     gene            complement(15147..15518)
                     /locus_tag="PK717_00075"
     CDS             complement(15147..15518)
                     /locus_tag="PK717_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOR46103.1"
                     /translation="MFNESMMAKLAKLREAGKDVYPPRVPKPTSEAETVKANFEKFEN
                     QTVTIAGRITAQRGHGKAMFLEVADETGPIQLYVRVDDVGEEIYGLIRDALDVGDLVV
                     VTGSVFKTRTGEVTIHTKEIM"
     gene            complement(15523..16041)
                     /gene="greA"
                     /locus_tag="PK717_00080"
     CDS             complement(15523..16041)
                     /gene="greA"
                     /locus_tag="PK717_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOR46104.1"
                     /translation="MSTKQNEEAKPQIKHGSEHLLTREGYKKLYAELEDLKNNKRQEI
                     AERIRQAKTFGDISENAEYDEAKKDQAFIEGRIQQLENILSDCEIIESEDVDLHEVNI
                     GCTVTVVNLSTHENMTVTIVGTYEAKPQEDKISNVSPLGRELLGHETGDVVRVVTPKG
                     DVKYRILKISRG"
     gene            complement(16054..16953)
                     /locus_tag="PK717_00085"
     CDS             complement(16054..16953)
                     /locus_tag="PK717_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOR46105.1"
                     /translation="MKKIVSVSLGSSSRDHAYTTELLGEKFEISRVGTDGDVDKMVEL
                     FKKLDLEVNAFGMGGGDIYLRPGNKTYVLREPYKWSKAAPTKPVCDGAYFKAVMEPLV
                     IHNLFQQGFLKKDMKVLVMTAVDRYFLAKAFADEGCRCVFGDMPFSLGIPLGVTSLGT
                     IRFLGTTLLPIITKLPYHWLYPLGSSQEKMKNKPNRFFAWADVVAGDLHYIRKNMPLD
                     MKGKIICTNTTTKKNLDEFKERGVKAVVTFTPDMNGRTFGANVLEAVFASILGKHPQE
                     TTEKEYQDLIGKLGIQKPNIVML"
     gene            complement(17085..17594)
                     /gene="amrA"
                     /locus_tag="PK717_00090"
     CDS             complement(17085..17594)
                     /gene="amrA"
                     /locus_tag="PK717_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOR46106.1"
                     /translation="MPDEFVELARKAIKAWVLEKRTIETPAPVPENMKGKAGVFVSIH
                     EDGDLRGCIGTFLPTTANIAHEIIEMAIEACSQDPRFMPIRPNELDKLEINVDVLSSP
                     EPIDNIIQLDPKTYGVIVSRGPRRGLLLPDLEGIDTAEQQVAIARRKAGIYDDGPVQL
                     QRFKVTRHT"
     gene            complement(17594..>20151)
                     /locus_tag="PK717_00095"
     CDS             complement(17594..>20151)
                     /locus_tag="PK717_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOR46107.1"
                     /translation="LVTVGAATPTPDAATKTGVWQYRRTLTVYEKGGVSRKSYAIDLA
                     LSKSELADTASDLRLTDNKGTLLPYQIVSIENGKVTVRIPVDLEPNGRKAFYIYYGNP
                     QAQPPQRVFVGSENMIGNDFVSLAIGEVKICSYSKDNKITVTDVAGNYIKDIFGRTIS
                     PETYPAGGIRSFKPENPTVIRIQSTGLCSVAMGNFAKEETDCTAFISGEALVFVPSFL
                     AITSLRDGNNVSVFNRGELAKKAILNSNQTLVFDNLPAGMRKIQSDYDCAIQYGTASS
                     YSFFSVPQKGLVYSYLPLGPTAIVAHQNNTELFVTWSDPTIPPQKFELSSAGQMQRLE
                     TKSPLIKKSEIYNATTITSNKPITVLAMGTQGGHGATFLPGVDGKLLSKRWNTVSGPI
                     DPKNPSNRNIALIAPYSKTTLSSPTERRVFPPLPSSPMSTSLVSVTTEFSPVLAQTNQ
                     YAVILDANPDDTALLYQVPPIKDKSVIFTLSDPEAAPEGSTWMPESPVSPGGEPGKQE
                     QPNENFFTSLWKTLTNPTENPMQFSLVLLFAALILITIAVLLWRRSKHSPEDDADDED
                     EEKTEAKEDNTTQPLYQDDGISQARVDFEALEELDKEPPVKPEEKAEEKDFSENWPKQ
                     EATKQTQAQQTTTKQPGENMRTPKLRLPNLSKFLSQEATNSINSSNETTAKEEPPCSH
                     ETPPPPAEERHEEAPMVMPSPMVQETKEPEEPEIDVTESEIAQPSVISEAVAQLADQM
                     NKMGVVADPGAIMRLHKEGLLPLLSKISIAHTAYSLLPGELSIDPRFERIPIMGKEAE
                     RANKISSELGVFEEVGRALVVAEKTRSEFYITTARLPERMGKLKVVHVERFIG"
BASE COUNT         5101 a         4493 c         4535 g         6022 t
ORIGIN      
        1 ccatgtaaag gaaaatttca aaaaggttga tgagatacct tttgattttg tgagaaggcg
       61 aatgtcagtt gtcgttgaag acgcctccgg caagcaaatc ataatatgca agggtgccgt
      121 agaagaaata ttttctgtgt gcacacagat ccagacccag ggtgtggttg aaaaacttgg
      181 ccaggaacac atgcaaaagg caaaagaact tgtcgagtgg tcaaattcaa acggtttcag
      241 ggcgcttggg ttggcataca gggaaatgcc aaaacgccag gaggcttatt gtgtctctga
      301 cgagcaggaa atgaccctcc ttggttttct caccttcctc gacccgccaa aggtaacggc
      361 aaagcaggcc ataaaagacc tcagggacct cggggtcagc gtaaaaatcc tcacgggtga
      421 caacgatctt gtaagcaaga aaatatgctc ccaggtcggg ctggaaactg gctcaatcgt
      481 tcttggcagc cagctcgaag agatggacga atcccagctc gtcgagattg tggaaaaaac
      541 aaatgtgttt gcaaaacttg ccccatcgca caaagagcgc atcataacaa tcctgaagaa
      601 aaaaggccat gtcgtcggtt tcatgggtga tggcatcaac gacgcaccag ccctcaaggc
      661 cgcagacgtg ggaatatcgg tggacactgc tgttgatatt gcaaaggaat cctcggacat
      721 catcctgctt ggtgagagcc tcactgttct tcaggatgga ctaatagagg gcaggagggt
      781 cttcggaaac ataatcaaat acataaaaat ggccgcttcg tccaattttg gaaacatgtt
      841 cagcatcttg ggtgccagct tcttcattcc cttcctccca ctgttgccca tccaaatcct
      901 cacaaacaac cttctttatg atttttcaca gacagccata ccaacagaca aggtggacga
      961 ggaatggatc cagaagccaa ggaaatggac cataggcagc atcaggcaat tcattatttt
     1021 catcgggccg atcagctcga tatttgatta tctgacattt tttgtgatga tatttatttt
     1081 tggcggactc gggaagccgg agctcttcag aacgggttgg tttgtagagt cgcttttgac
     1141 ccagacactt atcatacacg tcatcaggac caacaaggtg ccattcttgc aaagcagggc
     1201 cagccttccc ctcacgatca cctcgattgt catcgccctg acgggcatat acctcccata
     1261 ttcagctctt gcagggaccc ttggccttgt tggcctgccg ccgctctact ggccaatcgt
     1321 tttcgcaatg ctggtgtgct atgtcgtgct cactcaggta gtaaaaacct ggttcataaa
     1381 gaagtatccg gagaagtaga attttaggcg attattgcca ggttgtcttc aggattatat
     1441 aatcctgaag acaacagttg ctgttggctt gggaaggagc aaacaaccag tggaaaaaat
     1501 cgacctcaaa aaacagcttg cgggcctcta caacccgcca aaaacgccgg tgatggtgga
     1561 agttccgcaa ttcaacttca tcatggtgga cggaaagggc gacccgaaca cgtccgagga
     1621 ataccagaac gccatgcagg tgctctattc gctctcctac accctcaagt tcatggccaa
     1681 aaaagtgctg gaaaaggact acacggtcat ggtgctggag ggcctgtggt gggctgaaaa
     1741 catggacgac tttctggagg ggaacaaatc aaactggctc tggacggcca tgatcatgca
     1801 accggatttc atcacaaggg agcattatga agcaacgctc tccgaggtga aaaagaaaaa
     1861 gcccaacccg tcacttggga aggcaaggtt tgaggcgttc agggaggggc cgtcggccca
     1921 gataatgtac atcgggccgt attctgccga ggggccaaca atagctgcgc tccacgaatt
     1981 cattgacaga ggcggaatga agccacgcgg caaacaccac gagatatacc tcggtgaccc
     2041 caggaaggcc gaccctgcga agctgaggac tgccatcaga cagccagtgg agaaggctta
     2101 ggaaaaaata tttgtagatt tcatacaatc aaagattacc aatttcttgg gtaaacagca
     2161 tgaaaattca agagaattat tgatatcagt gatgtgatac gcgcttttaa aaaatgtata
     2221 tttgtttata ataaaaacat gtggaagaaa atccttgtta taagtgcttt tattgtaatc
     2281 tcgatattaa tactttatct tattaggtat ttccttttcg cactttgcct catgtttctc
     2341 caagtttgcg taaaaaatgt cttttttatt actattgcca tgatgattta tttcccaatt
     2401 tactgtttat acagagtagt tttcaagata aaagatgaac aaggccacgt gccattttat
     2461 tccagaagat ggtttatcaa gctcgtttgc gggtttatta ctattgcgtt tgatttattt
     2521 tttatccatc cactatttaa tggttttaag cacttgtata tcgaaaggac tttgattgtt
     2581 tttgtcacaa tttatatttt gtttattcta tatgttggag cttttgagtt ggtttattgg
     2641 gagaaataag aaaaataact aaaatatttt tgatcccctt attttttatt acaaatctga
     2701 atcttacacc tactttataa aagaagaata ttctatataa taaaaacatg gggaagaaaa
     2761 aaatctttca atatgttttc tcgatatttt tgacactagc acttgtaatt gcttattttt
     2821 ttatcttcaa accggcatcc cacataagcc ttaaggatat cctttctgtc gtccttttga
     2881 ttacggtgta tcttaccatc tactatttgt ataggctaat ttttaatgta aaaatcgaac
     2941 aaggccacgt gccgctttat tccagaagat ggtttattag actcgtttgt ggatttatta
     3001 ccattgcgtt tgatctgttc cagatctatc cgttatttga tggctttgag caaacaagta
     3061 tcgatacaac cttgattgct tttgcggcat catacggatc gtttattttc tttgttggag
     3121 ttcttgagtt gatttatttg gagatgaaaa agaataatta aatgtctttt tgaatcccct
     3181 ccttttattg caggtattat atctattcct taaaagagaa tattttgtat aataaataac
     3241 atgtggaaaa aaatccttct tctaagcatt ttattgctga ttgcattaat agtattcttg
     3301 cgattgtgtt attttactat tagcaacgat gacgtatatg cttatattag aacgggagaa
     3361 tttccttgca ttgtactgct tgctattttg ctcagccgcc cagtttatta tttgtacaaa
     3421 cgaactttca aaaaaaagat tgaaaaaaat ccagcatctt tttattccag gagctggttt
     3481 ataaggcttg tttgcggatt tgttgtatta tcaattgatt tcactctagc ctatctgttc
     3541 catggtatta aatgccatac tttcatggaa atggcatgga ccatttttgc cttatcatat
     3601 gggctatttg tcatgtacac cataattatt gaactcatag agagtctttt ggtgacgcta
     3661 gaggccatag agaaaaacgg ctgatctttg taaaaacctc acggtccaac acaaaaacat
     3721 ggctggaccg tgagtccgca aattgcccct tttggttggt ttgagcactt cctacatcgt
     3781 cacggctgtc cttcgagccc ctcgccttcc tctgtttccc cctcctcgtt gcggatcctg
     3841 tatctatact gattgccttc gccttcctcg ttctcttcac cttcttcacc aggcggcatc
     3901 tggaattcct ggccattctg gaaaccttct ggcggccttc ctcttccttc gccatgttcc
     3961 ctggagctcc agaaaccaac accttgtgcc agctggatca atccgatgaa aagaacaaca
     4021 aaaaggattc ctgcgatata aggctttatt ttcatttttt tcaccatcca atcttgaagc
     4081 tgagggccgt cgggcattct tcaacgcatt tcatgcagct gatgcactgg cccctgtcta
     4141 tttttgtctg cctgctgatt ggtatctgtt ttgggcagac ttcttcgcac cccttgcatg
     4201 acgtgcaatc ttcatttgag tgcacaacgc ctgttgccga gagctttgca aacgggagaa
     4261 tgattgcgcc aagcgggcaa aggtatctgc agaagaacct ttcgataaac atcgagccga
     4321 tcaggatgag caccaggacg acaaggccaa cggcaaacac cctgccaaac gagaaaagat
     4381 tcaggaacgg gtcgactgtg ccgaacacca gaaagccaat tgagaaggtc atgtagagga
     4441 tggcggcaag gacgacgtac ctgaaccaga ccagaacatt gtgcaatttt tctggaacaa
     4501 ttattctctt tttccagaat acccttctta gcatgaaaag ccattccgtg accgaaccca
     4561 cagggcacac ccagccacag aacgccccgc caaacgccag cacggcaagc agaagtgccg
     4621 ccagcatggc aaagtttgac aggcctgttt catggacaaa ctttagaccc ccagaagcaa
     4681 gaaatgttgg caacgtctct accccaccaa agggacagat tccgtgtagt tcgattcccc
     4741 tgaccccagg aaaaataaat cgccccagag tgtaatacag aatcagaagt acaaagccaa
     4801 tttgggagag atgacggatg agtgcatacc ttttttgttt cattctacaa aaattcctcc
     4861 tgacttttta ttgataaaat cgccatgatt tctaatacta cgatttatag taaaaagtca
     4921 ggtattcggt atgatactga tgcaatagtt tcctgatcaa cttttttggg tattttcaaa
     4981 aaaactgatt ataaaaaaat tggaatatta aaaaggttaa aaataaccat tcaaataatt
     5041 atcataatgg ataatttgat ttaacgatca attcttgatg tcaaataata cgatgctact
     5101 caattgagca gaaaggaggt ggagggatgc catatcagca caatataaac tcgataaagg
     5161 tatcggttga aagtggcctt gttaatacag aaatcatgga acccatttct ggtttgtcag
     5221 ttgtctggat tgaccaacaa aactacaatc ctgctaccat ggaagtctgc tcgtgcccaa
     5281 attataggtg ctggaccaat aatgcaatgg tctgatggtt ggtgtttaaa aaaatattgg
     5341 gggagaaata gtcaatcgtc tcccccaata ttttttgagt ctatagagga gattatgggt
     5401 cttaggtttt caaaatttat tattgccaaa gaattggatg aaaataaata cgttttgatg
     5461 tctacaatat ccggttccat aattataatt gataagaggc tgatcagtct cataaacagt
     5521 ggcaacttac cggaagaaga gaaaacttgc aaggaattgt caaatgcgtt ctttttggtt
     5581 gacgaaaatt ttgatgagaa atcctttctg attgaaaaat tttccagaga aacatggaaa
     5641 aatgataccc ttgatataac gatagctcca tcttttgcat gcaattttgc ttgcccgtac
     5701 tgttatgagg ccagagatga tcgcatcatg gatgaaggga cattaaaaaa tctggtcaga
     5761 ttcattcagg gccatctgaa aaagtttaac atcggtcatt ttatcatggc ctgctatggc
     5821 ggggaacctc ttttgtttcc tgatcatttt cgatatattt atgaggaaat ttcaaaaaca
     5881 aatccttcag taaagatatc tccgagaatg accaccaatg ggtcgttgct gaggaaaaag
     5941 cttggtggat tcatgaagtc tgtgccattg gaaaggatac aggtgacaat ccacggggat
     6001 aaaaagaatc atgattcaaa aaggttctat gtaggtggaa aaccatccta cgaagatgtt
     6061 gtgaatggaa taggggaagc aatcaataga aagatcccaa atattgagat cagggtaaat
     6121 attgatctgg agaacgcctt ttcactgaaa ggccttttaa tggatttgga caaaaggggt
     6181 ttttcgaatc atgagaattt gcgtgtttat gcatatcctg ttgtccatat gacgccggag
     6241 tctcttggat acaaaagcaa atgcctatct attgatagct actttgaaaa gataaccgga
     6301 atcattgagc tgatcaatgc ttatggtttt tgcgggggag ccaagcacat aagaaaatac
     6361 ataaaaaatc ttgtgttgtc aaatctttgt ggtgttggca cgaagggttt ttatacagta
     6421 tcgcccgatg gcgcaatcgg gacttgttgg gaatttattg ggagagaagg ctttgagata
     6481 ggaaatgtaa atttcagtgc tcctattgac tgcatgaaag aaaaaatgtg gattgatgct
     6541 aggatctcaa aggtcaagcg ttgcctggat cttgaatgtc cgcttgtccc cagttgtgct
     6601 gggggatgtg tggcccagag cttttctgaa aggggagacc taggcgaacc tgcttgtttt
     6661 aagaaaccgg agcaaatcgc aggatatctt aaattctatg cggatatgag catgagatat
     6721 tcgatctgat ttttatttgg tgtttttatc tcgcctcctc cacctcgccg tccttgtgga
     6781 gcattagctt gatgcctttg ttgatgaaga cctccttgat cagctcgctg tgcgggtagt
     6841 cgtcatggta atggagctct tcaaggtttg gaaaggtgtt tgcaatgacc tttgcacagt
     6901 ggaaacacgg cagtgtcgtg caaaaaatct gggtaatgtc ttttcttggc ccctcatacc
     6961 tcgagattgc gttgatttca gcgtggatga ccgggcactg ccagaggtct gatccggaag
     7021 ccaggagttc cctctggcag gtgtcgcagt gggtcattcc tgccacaacg ccgttgtagc
     7081 cagttgcttt tatattgccg tctgaatcca ccagcacaca gccgacctgg cgcctgagac
     7141 atgttgacct tttggcaacg atgtctgtta tcatcatgaa atatgttggt ttgtctggtc
     7201 tcatggtttt gccggatatg ttatcactac tgttttggtt gccgctttcc attcaatgga
     7261 tgcgccaagc gcctctgtta tgaaccttgt cgggacgaat gttgagccac caatgacttg
     7321 tggcggaggg ttgactgaaa ccggttttcc gtcgacggat gcggttgtct tgccaatcca
     7381 catctcgact ttcttgccct caagcgtgta tgtgactttc ttggtctggt cgtcccagcc
     7441 gatgtctgcc cccaggcttt cgccaaggaa gcggaaagga acgaatgtct tgccgcttat
     7501 gatgactggc gcctgaggca gggcattctt tttctcttca ccgtttaccc aggcattttt
     7561 ggagccaacc atcaagatca cggtagtgta ggcggagaag ctgaccttgt atgtgaattt
     7621 tgtctggttg ccacccggat caaatactat gacggtgatt gtattggaac caacccttag
     7681 caccgttttg gcctggaatt tgccatccgg gtcgattatt gcaagttccc catttacgat
     7741 gatctgcgag tttggttcgg caacacctgt aacatcaagt acctgcgtcc ttgtgaccaa
     7801 gccgtccttg aggttgacga caggttttat cgggtttggc atgaccatta tctcgtttga
     7861 ggcatcagag aggttgccgt tctggtcgac tgacttgatt atgtagtaat agatcaagcc
     7921 attggtgacg tttgggtcgg tgtattgtgt gccggtgaca tagaaatcgg ttattgcaac
     7981 atccgtataa atccccgggt tgtttgacct gaatatgtag taaccaacaa tgtttggtga
     8041 tgcgattggt ttccagaaaa ggtgggccct gccatctcct ggatagccga cgaatatttc
     8101 tggcgtggat ggcgcaggag aaccggcgat tacagtcagt ttgactggaa cttcaatgac
     8161 cggctgtttt tcatcattgg atgtaacgat gattgttgcg aactggtcgc ctggcgggag
     8221 gccttttgct gatgctttta tctgtatccc agtgttgttg ccaggagaga cgatgccgtt
     8281 tgtggggttg tatgaaagcc agtccttgac tgttgtggtc gccacctgaa gaatgactgg
     8341 tacctgaaca aggccggcat ttgaagccag aatgacattg cccctgtaaa gctgtcctgt
     8401 gaccattccg tcaagcttga cggtcactgt tacagaaaca accgttttgt ctatattgcg
     8461 tatgaggcat tttatccatt caacatcgct tgaggccgtg acctggagat attgttccgt
     8521 tggtgctgta catgtaacag tttgcgaaac ctcgcctttg gagggtacag tgccgaaatc
     8581 caggaaactt ggatacacca gcatatttcc atctggtgat gccagaggct gtgtttttgc
     8641 cttgctgtag gactttgaga gcgaaaacag gagggagctg gaacccctgt tgaagatgcc
     8701 cattgtggct gttgccgttt caccctgctt cgcgacaata ttgatttctt ttggagaaag
     8761 ctcgatcagt ttgtcatcct gagagagggc tgtctgaaaa cacggcatta ttaccagagc
     8821 acacaacaaa aaagctgtca gtttcttcat ttaaaggcca cactccttcc catgcacttc
     8881 tatcaccatc ttaggcactt taagtgccat gtcaaaacct ttctatctta tttatttcga
     8941 tacccagcaa cttacttgcg agttcacaaa atttccctgg atcgccggag gtccacagac
     9001 tgaccctgcc attgccttcc agtatgttgt tttgcattat tacccttgca agctgtttgg
     9061 caactggcgg gccagtatcg ataatattga ctttcgaggg gagtattttt ctgagcgcac
     9121 cggcaaggaa cgggtagtgt gtgcacccaa gcaccagctg gtcaaaacct gttttcatat
     9181 acggtaacag tatttccgag aggcgttcct caacctcttc gccctcgcat tttccctgct
     9241 ccacaagctc cacaagacca cttgagggta tcattattat gtccttgtct tgggcaaaga
     9301 ggtttacaag ctggagaaaa cgctccccaa gagatgtcgc ctttgttgcc agaacagcta
     9361 tcttgccgct ttcagtcagt gttgttgccg gttttactcc tggctcgacg ccaatgaatg
     9421 gtatattggg aaattctgct ctaagcgtgg agattgcgtt tacagtcgct gtgttgcagg
     9481 caaccacaat tgcttttgtg ccaagcgata tgagttttat tgtaatcagc ctgcttctct
     9541 cgattatgaa ctcttttggt ttctcgcccc atgggcagta tgcctcatca ccaaaatagg
     9601 cataatcgtg atttgggagt atttctaacg catttttgag gatggagagt ccaccaaccc
     9661 ctgaatcaaa gaaacctatc atttcagggt ccctccctca cattccggaa ctccggcaga
     9721 tgtttcgact tcattctcat tttgaggtgt acaaataact ggtttcactt ttttgccaag
     9781 tgcccagaag gcgatcggca agccgacaag catgtttaga tggtagtcta ttagtctgta
     9841 aacgagcacg aacggtgaaa gatttcctgg gtttgcaaca agccccctga aaagagttcc
     9901 aaggcctatt tctacaccgc cagaggcacc tggtgttggc acccagattg taagcagctg
     9961 cgatattatt gatgttgcga caacaatcat cagcggaatg ctctctccaa aagaaagcaa
    10021 aacaaaatat ggaacagttt tcagaaggaa ccatgagaca aggctcgtaa aaattgtcag
    10081 ggtaagataa actttgttcc ccccgaaaat catctttgag gaatttttca ccgagtcgag
    10141 gaacctgtag ccttttgcca tacattcatc aaccgtgggc gctttgacga gttttctcac
    10201 tagcctaatt cgcgaggcga tattaattaa gaaggaaaaa aactttgggt tgaggagtgc
    10261 ggcaatgccc attgcgatca gcgctatcat cattatcagg gttaaaacga atgtccaccc
    10321 gataaaactt gacagattgc tatcaaaccc aaataaaact gccacagcta ctgcaaggac
    10381 aagaaatatt cttgttgata cagatattat tcctctggca ataaccatta cggtcccttc
    10441 tgcaatagag aaaccctgat cgagtaaaaa gtatatctgg aaaggttttc cgccagatgt
    10501 tgcaggtgtg acattcgaaa agaagttgcc gataaaaaat atcaacatca ttccccaaaa
    10561 gctcggtttt ttcccaagcg ttttggcaag caggtaaagt tgtagggcct ctgtaaagta
    10621 cgcagagacg ttaattgcaa tggcggcaag tatgaaccac aggtttgttt tgccaaggga
    10681 ttttaaagcc gatccaaaat caagcccggt tgcaaactgc attacaaaga gaacagcaat
    10741 gattgaaagt gctcctgcat agagcatgta gcgaatgatt tgtgcctttg tgatttttac
    10801 cattttttca actagaacat tataacaaaa aaagccccaa aatcattggg gcttttttcc
    10861 ttcgatttcc ctgcctcaat ttaccttgtg ctctgaggca agttcttctt gagccatctt
    10921 ttctttttct tcttccattt tctggcttat ctggctggtg agtgctgata aatcggcatt
    10981 gctggtatct tttccgagct ttttagacaa gagcctcaaa gctatgggtg cgccaacaag
    11041 aacatccatg tggtagtcca tcagcctata aataaataca aacagtccta tgtttccagg
    11101 agtctgcatg gttgatgcaa acgcaagcat tccagtttct attcctccga ctgcaccggg
    11161 tgttggcacc catgcggtgg cgagttgtga gatcacacct attgcaaaaa taacaaatat
    11221 atttgggtgc tcgtttaggg caagcatgat aaaaaatggc atggttttca ggagtaccca
    11281 tgtcaggatg gaattgaaaa tcgcaccaat catccatatc tttttccctt gaatgagcag
    11341 ttttgctgat gatcttattt cgccaaggaa aagctcgcct ttttgaataa attgcttggg
    11401 atcttgtatt tttgccagct tgcggatcca gcggaacctt gaaaagaatt tgaccaaaaa
    11461 cgaaaaaata tgtgggttca tgataattat gaatccagcc accacaagaa gcgcaaatgc
    11521 cgctagagta atgtagaaaa tcaaatcaaa tgtgaaacta aggtttaacc tgaaaattcc
    11581 caatgctatc agggccactg ttacgagcac atagagaatc ctcaccatta cagatatgag
    11641 gcccctgact gctaccatta ccattccctc tgccacagag aagccgtcgt ccatcatgaa
    11701 ataaacctgg aaaggctcac cacccgatgt cgaaggtgtg gcatttgaaa agaagtttcc
    11761 tacaaaatat atctgcatca tccgccagaa acccggtttt ttgcccaatg ccctggagag
    11821 aaaccagagc tggaaagcct cgcagagaaa cgatgcaaca ttgcatagtg ctgcagcgag
    11881 tatccaccac cagtttgctt ttgagactgc ttcacccatt tttgagaagt caaagcccgt
    11941 ggaaaattgc atgatgatga agatgattac aaaagacatc aaaccggcaa caacaatata
    12001 tcttgttaga gttttggcag tcatcttggc catgtgtcta gtcctcctgt tttagattct
    12061 gcattcgccg ccggaagcgg aaacaatgaa ggcttcgtca cctggctgga ttatttcatt
    12121 ttgtgatgga tttaccatca tattgccact ccttattagt gcaaccacaa tcatttctga
    12181 ttgactataa agtgccatga cgagttcctc ataccttttc ccatgaaaag gcgcagggat
    12241 agatattctt ccaagcgaac cgggtgattc ggtttttagc aatatttgca ttaattctgc
    12301 cacaccagga ttagacacag aacgcgacaa aaggtctcct gcaacccctg acctgacaat
    12361 aatctcgtcc actcctgcat gacgcaggta ttctgcgtct tcttcaagta aaacttcgca
    12421 gatgaccctg cacctgtttt tggaaaccct ctttactgca agtgccgtaa gtatgacttt
    12481 tgagtcggtg tttctggcgt tgatttcaaa atcggccagc acaacggctg cttttgcgtg
    12541 ttctgcagat gccctgtcga ggttttcaag tctcgttggg tcacctttaa tgaagtatgt
    12601 atcttgctgg attgaggatg gtggttcaat gtctgccatt acaacgattc ctatctgttt
    12661 ttcctggcat tccctgtaaa ggtctccgat gattctcagg ccattctcat tccaccccaa
    12721 aatcaacaca tgatccctaa atctttcgac cttattttga ttcattcttt tgaccaccct
    12781 ttcaatcagg gtgctggcca gcgtggcagt aaggccagct atcatgatta ttccggagaa
    12841 catggcaaga atggctatca ccctgccaat tgatgttatg ggtacaacat ctccataccc
    12901 tacagtggac attgtgacaa cagtccacca cactgcgtca aggaaatttt ttattgagga
    12961 gcctggcaat cccccttcgg caacatagat cgctgaggca agaataaaaa ttgggataac
    13021 aacaatcccg agccatacaa aaaccctttt tgtggctttt ttgagaaggc tagatttttt
    13081 cgccacagtt cgggcagaac tttgcgcctt ccggtacaga ctggccgcac tttttgcaat
    13141 ttaactgaag atttttccca caattggggc agaatttggt gttttgctgt atgggtttgc
    13201 cgcactcagg gcagatctca tttgaaacct ttgcgtcggt cccctttgag aggttctcga
    13261 ctattttgcc acccattaca aaccctgcac ctatttgagt agctgcaccc atcattcctt
    13321 cgccaccctt ttccatgccc tttgcggcct tgtatgccag ataggcattc atgtctttta
    13381 tgacacccat cccggccctc tcgtctattg cagtttcgac ttctggcgga aggcctattg
    13441 attcgataaa tgcagcgagg caggaaagtc cgaatttgga cacttcaccg tttatggatt
    13501 ttgaaagctg gtcaccgatt tcctggtaaa tttttggaag gtcaaagacg gtggtcaggg
    13561 actcgccgag catgtcggta aattttgagg ccacaatacc gcgcatgaaa tcttcgattt
    13621 tgcctcgggt cataaaaccc tttgtgccca ctatttcagt aataaatttt cttgggtcgt
    13681 ccactctcag ggtatagttg ccgaatgctt ttacccttac aacgccgagt tctttatcgc
    13741 ggaatggtat tgaatcaggt gttccgaatt tgaaaccggg catctgtact gttgaaatga
    13801 aaagtacctc tgccctaaag ggagacttga agccgtaagg aattgaaagc aattgtgtca
    13861 gtatggggat gttctcggta gaaagtgtgt atttgcctgg gccgaacatg tcggccactt
    13921 tgccgtttga catgaagatg gcaagctggc cctctcgaac aataagttgg gcacccattt
    13981 tgatgtctgc agaaccctgc ggcggttccc tgtggagcag ttcaacgccg gagttgtcca
    14041 accattcaat tatttcaaga aatatcccca ctttgcctcc tgagttgagg gaatgcgaga
    14101 acttccctta ttgaaacctg atctgttaaa agcataacaa tcctgtcaat gcctatccca
    14161 agtcctccag ttggcggcat gccaactgct agtgtttcaa caaagtctgt atcggtctga
    14221 tgtgcttctt catctccact gcttctggct ttttcctgac cttcaaatct gttcaactgg
    14281 tcttgtggat cgttgagttc agagaaagca tttcctactt cataacctgc gatgactggc
    14341 tggaaccttt caacccacat ggtctcgcct ggaatgagct ttgctagtgg cgaaagctct
    14401 atcgggtaat tgtagataaa ggttggattc ttgatttttg gctcaacaag ctttttcagc
    14461 gtttcgtcgc agaaaccgcc aaaaccaggc ttcttttcca tcttgacgcc attttcctga
    14521 aggaaagcaa atgcaaactt tgcgtccctg agtttttcga tctcaacgcc ggcatattcc
    14581 ttgaatgcct ctgccattgt caatcttttc catggcggtg tcagatcgat ttcctcaccc
    14641 tggaaattga tgacaaaaga cccagtgatt gtttttgcga cagaagaaac catcctctcg
    14701 gtgatgtcca tcatgtcgtg gtagtctgcc caggcctggt aaagttccat gattgtgaac
    14761 tccgggttgt ggacaagtga tatgccttca tttctgaagg ccttgccgat ctcgaatact
    14821 tttggcattc caccgacaat gagccttttt aggtagagct ccggggcaat cctgaggtaa
    14881 aggtccatat caagcgcatt atggtgggtc atgaatggtt ttgccagtgc accaccatag
    14941 agcggctgga gtgtcggggt ttcgacttcc tcaaaaccct cattccacaa aaactctctt
    15001 attgctctga ttattctggt tcttttgctg aaacgctccc tgacctctga atttgcaatc
    15061 atgtccagat ggcgtttcct gtatcttatt tcggagtctt tcagaccatg ccatttttct
    15121 ggcagtcctt ccagcgcctt ggttgctcac ataatctctt ttgtgtggat ggttacttcg
    15181 ccagtcctgg ttttgaacac cgagccagtc actacaacga ggtcaccaac atcgagtgca
    15241 tctcttatca ggccatagat ttcttcgcct acatcatcga ccctgacgta aagctgtatt
    15301 ggtccagttt catctgcgac ctcaagaaac atcgccttgc cgtggccgcg ttgggcagtt
    15361 atcctgccag ctattgttac agtctggttt tcaaattttt caaaattggc ctttactgtc
    15421 tctgcctcgg aagttggttt cgggaccctt ggcgggtata catcttttcc ggcctcgcgc
    15481 agcttggcca gcttcgccat cattgattca ttgaacattg tcttatcctc tggatatctt
    15541 cagtattctg tattttacgt caccctttgg ggtaacaacg cggacaacat caccagtctc
    15601 atggccgagc agttccctgc caagtggaga cacgtttgaa atcttgtcct cttgcggttt
    15661 tgcctcgtat gtgccaacga ttgtcactgt catattttca tgggtgctaa gattgacgac
    15721 tgttacggtg cagccaatat tgacttcgtg caaatctacg tcttcggatt caattatttc
    15781 acagtctgaa aggatatttt caagctgttg tattctgccc tcgatgaatg cctggtcttt
    15841 tttggcctca tcatattcgg cattttccga tatatctcca aaggtttttg cctgcctgat
    15901 tctttcggca atctcctgcc ttttgttgtt tttcaggtct tctagctcag catacagttt
    15961 tttgtagcct tctcttgtca gaaggtgctc gctgccatgt tttatctgcg gttttgcttc
    16021 ttcattctgc tttgtgctca tgatgtcctc tccttagagc ataactatat ttggtttttg
    16081 aatgcccagt ttgccaataa ggtcctggta ttctttttca gttgtttctt gggggtgttt
    16141 gccaagtatc gaagcaaaaa ctgcttccaa aacatttgcc ccaaatgttc ttccattcat
    16201 gtcaggagta aatgttacaa cagcctttac acccctctct ttgaattcgt caaggttttt
    16261 cttcgttgtt gtgtttgtgc agattatttt gcctttcatg tcgagtggca tgtttttcct
    16321 tatgtaatga agatctccag ccacaacatc tgcccatgca aagaacctgt ttggtttgtt
    16381 tttcattttt tcctggcttg agccgagagg gtagagccag tggtatggga gcttagttat
    16441 gattggcagg agtgttgttc caagaaacct tattgtcccc aaactggtga ctccaagtgg
    16501 tattcccagt gaaaatggca tatcgccaaa aacacacctg catccttcat ctgcaaaagc
    16561 ctttgcaaga aaatacctgt ctactgccgt cattacaagt accttcatgt ctttttttaa
    16621 gaacccctgc tggaacaggt tgtgaatgac cagaggttcc atgacggctt tgaagtatgc
    16681 gccatcgcaa acaggttttg tcggtgcagc tttagaccat ttgtaaggtt cacggaggac
    16741 gtatgttttg ttgccagggc gaaggtatat gtctccacca cccatgccaa aagcgttgac
    16801 ttcaagatca agctttttga atagctccac catcttgtcg acatcgccat cggttccaac
    16861 ccttgagatt tcaaattttt ctccaagaag ttcagtcgtg taggcatggt cgcgtgatga
    16921 cgaacctaaa ctgacagaaa caatcttttt cacaaggcct ccttgaacaa actgatatta
    16981 tatcttaaaa gccattaagg taaatataaa aaactgggcc accattgtga cttggttctt
    17041 tagactgttt cttccttaaa taattacagg tgaattgact ttttttaggt gtgccttgtt
    17101 accttgaagc gctgcaactg gaccgggcca tcatcatata ttccagcttt tctcctggct
    17161 atcgccacct gctgctctgc tgtgtcaatg ccctcaaggt caggcaggag aaggcccctt
    17221 cttggtcccc tggaaactat caccccatag gtttttggat caagctgtat aatgttgtct
    17281 attggctcag gtgagctaag cacatcgaca tttatttcaa gtttgtccag ctcgtttggc
    17341 ctgataggca tgaatcttgg atcctgggaa catgcctcaa tagccatctc gattatctcg
    17401 tgggcaatat tggctgttgt tggcaggaag gttcctatgc accccctcag gtcgccatct
    17461 tcatgtattg aaacgaaaac accggccttc cccttcatgt tttcgggaac aggtgccggt
    17521 gtttctatgg tcctcttttc aaggacccat gcttttattg cttttctggc cagctcgaca
    17581 aactcgtccg gcattatcct ataaacctct ctacgtgtac aactttgagt ttccccattc
    17641 tctccgggag gcgtgcggtt gttatataga actctgatct cgttttttct gccacgacaa
    17701 gcgcccttcc gacttcttca aataccccta gttctgatga gattttgttt gctctctcgg
    17761 cttctttccc cattattgga attctctcga atcttgggtc tatcgacaat tcacctggaa
    17821 gtaatgaata agcagtgtgg gcgatggata tttttgaaag aagtggcaat agaccttctt
    17881 tgtgtaatct cattattgca ccaggatcag cgacgacacc catcttgttc atctggtctg
    17941 cgagctgtgc tactgcttca gatataactg acggctgggc tatttctgat tctgtgacat
    18001 ctatctctgg ctcttcaggt tcttttgtct cttggaccat tggtgatggc ataaccatcg
    18061 gtgcctcttc atgcctttcc tctgctgggg gtggaggtgt ctcgtgactg caaggaggct
    18121 cttcctttgc cgtagtttcg ttgctggaat ttatagaatt ggtcgcttct tgtgaaagga
    18181 attttgagag attagggagc ctcagttttg gggttctcat gttttccccg ggctgttttg
    18241 tggttgtttg ctgggcttgt gtctgctttg ttgcctcttg ttttggccaa ttttctgaaa
    18301 aatctttttc ttcggccttt tcctcaggtt ttacgggggg ctctttgtca agctcttcaa
    18361 gcgcttcaaa atctactctt gcctgactta taccgtcatc ttgatataag ggttgtgtgg
    18421 tgttatcttc ttttgcctct gttttttcct cgtcttcatc atcagcgtca tcttctgggc
    18481 tatgctttga tctcctccag agaaggactg cgatagttat tagaataagt gccgcaaaaa
    18541 gaagaacaag cgagaattgc atcgggtttt cagtcggatt tgtcaatgtt ttccagaggc
    18601 tggtaaagaa attttcattt ggctgttcct gcttgcccgg ctctccccct ggggagactg
    18661 gcgattccgg catccaggtc gacccttccg gtgcagcttc cgggtccgaa agagtgaata
    18721 tgacgctctt gtcttttatt gggggaacct gatacagcaa agccgtgtcg tcaggatttg
    18781 cgtccagaat gacagcgtac tggttggttt gagccaatac aggagagaat tctgttgtga
    18841 cactcaccag tgatgttgac atcgggctcg atggaagtgg tgggaaaacc cttctttcag
    18901 ttggcgatga cagtgttgtt tttgaatagg gggctataag agcaatattc ctgtttgagg
    18961 gattttttgg gtcaattggc ccggataccg tgttccagcg ttttgaaagc agtttgccat
    19021 caacacctgg gaggaaagtg gcaccatggc caccctgagt ccccattgcc aaaacggtta
    19081 tcggcttgtt cgaagttatt gttgtggcgt tgtaaatttc actttttttg attaaaggcg
    19141 attttgtttc aagcctttgc atctgccctg cagatgagag ctcgaatttt tgcggcggaa
    19201 ttgttgggtc ggaccatgtc acaaacagct cggtgttgtt ctggtgggca acaattgcgg
    19261 ttggaccaag tgggaggtag gagtaaacaa ggcctttttg cgggaccgag aagaatgagt
    19321 aagagcttgc ggtgccatac tggatggcgc aatcatagtc tgattgtatt ttgcgcattc
    19381 ctgccggcag gttatcaaag acaagcgttt ggtttgagtt caggatagct ttttttgcca
    19441 actcgcccct gttgaatact gacacgttgt ttccgtctct gaggcttgtg atggccaaaa
    19501 atgatggcac aaaaacgaga gcctcgccgg agatgaatgc tgtgcaatcg gtttcttctt
    19561 ttgcgaagtt tcccattgcc acagagcaca ggccagtgct ttgtatcctg atcactgtcg
    19621 ggttttcggg tttgaagctc cgtatccctc cagcagggta ggtttcgggg cttatcgttc
    19681 ttccaaaaat atcttttata taattaccag cgacatcagt gacggttatt ttgttgtctt
    19741 ttgagtagga acagattttg acttcgccta ttgccagtga aacaaaatca ttgcctatca
    19801 tgttctcgga gccgacaaag accctttgcg gtggctgggc ttgcgggttt ccgtagtaga
    19861 tgtaaaaagc ttttctaccg tttggctcta gatctacagg tatccttacg gttacttttc
    19921 cattctctat tgagacaatc tggtatggaa ggagagtgcc cttgttgtcg gtcaatctta
    19981 ggtcagaggc tgtatcggca agctccgatt ttgaaagcgc aaggtcgatg gcgtaagatt
    20041 ttctggaaac accacccttc tcgtacactg tgagcgtccg cctgtactgc cagacgccag
    20101 ttttggtggc cgcatccggt gttggtgtgg cggccccaac ggtgacaaga a
//
LOCUS       DAOSRI010000002        16409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_3636_length_16409_cov_4.247829, whole genome shotgun sequence.
ACCESSION   DAOSRI010000002 DAOSRI010000000
VERSION     DAOSRI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16409
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_16409_cov_4.247829"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PK717_00100"
     CDS             complement(<1..550)
                     /locus_tag="PK717_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOR46108.1"
                     /translation="MKELFRLIPQVSSILEDEDFKKEYGHFDQRITSSLIREALDSVR
                     DKIAGGEALDESQVKKLVETTLKKRLSPLTKRSLKRVINATGIVLHTNLGRAPMLPNL
                     YLAPLLEGYCNLEYDLAEGKRGKRGVHVKSVIKMLLGAESTVVVNNNAAAILLLLRTH
                     AEGKEVIVSRGELIELGGSFRLP"
     gene            complement(572..1000)
                     /locus_tag="PK717_00105"
     CDS             complement(572..1000)
                     /locus_tag="PK717_00105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR46109.1"
                     /translation="MEPEYKGTDSNCFACGTSNQRGLKLKFALIDDHVEARFVPDKDF
                     EGWDNIVHGGIVSTLIDEAMSHAIFRLDGGSVVTAEMTVRFVKPMFVGKPFVVHGKID
                     GLKGRLIEASANITDQDGNLIAKGTGRFIRVGINQKLNFC"
     gene            complement(1000..1395)
                     /locus_tag="PK717_00110"
     CDS             complement(1000..1395)
                     /locus_tag="PK717_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR46110.1"
                     /translation="MDRIKDILGKAKVIAVVGASLDECSASFPITKYLITRGFGVYPV
                     NPKYAGEEISGVKFLASLSDIKEKIDIIDVFRRSEAIPGMVDDFLAVRPGVVWFQLGI
                     YAPDSFKILEENGIEVIYDRCIYKELMEC"
     gene            1474..3306
                     /gene="dxs"
                     /locus_tag="PK717_00115"
     CDS             1474..3306
                     /gene="dxs"
                     /locus_tag="PK717_00115"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR46111.1"
                     /translation="MEILGRIKGPGDLKKLRTRDLGKLACEIREAVVDAVSKNGGHLA
                     SNLGIVELTIALHRIFDSPKDRIIFDVGHQCYAHKIITGRLDRFGSIRKKGGLSGFPN
                     PDESQYDLYKTGHASTAVSLAAGEAIARDLKGEKHSVIAIVGDGSLTGGECFEALNHI
                     GQMDTDVLVILNDNQMSISPSVGALSLFTSKLRAAIFYRRFSHYVGKGFSRMGRFGEW
                     AVGMGFRIKGALKRFLMADQYFEQLGFRYLGPVDGHDIPLLLAFLGRIKHMKGPVLLH
                     VVTKKGKGCSFAESKPTEYHGTAPFKPANGETANGKPTYSKVFGECMVEAAKKDKSIV
                     AITAAMSDGTGLAEFQRVFPGRLFDVGIAEQHAVSLASAFARNGLKPVVAIYSTFLQR
                     AYDQIIHDVALMNLPVIFAIDRAGLVPDDGPTHQGIFDLAYLSLVPNITVMAPSCAEE
                     LCQMLEIAFSSKGPSAIRYPKDVALKTDCEDVVFGKAELTKNGDDILLAYIGTFRQEV
                     ATISEMLEKDGFSCAVLNMRFASPLDTDSMLKHAKGKKVVFFIEEGVTGGGIGQRLME
                     HIPPLRIIGIQNAFPENGSRKELLESQKLDTTSIYEKIKASLSK"
     gene            3429..3731
                     /locus_tag="PK717_00120"
     CDS             3429..3731
                     /locus_tag="PK717_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOR46112.1"
                     /translation="MIEDTKPVMTVGAVADVLGVCEQTLRLWEQKGLITPNRVNGRRL
                     YSARDLERLELVKFLINEKKMNIPGVKKFLEIFDNCKQKNCEDVDECLKLWDKEGK"
     gene            3728..4906
                     /locus_tag="PK717_00125"
     CDS             3728..4906
                     /locus_tag="PK717_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR46113.1"
                     /translation="MNSIYLDHSATTPLDGEVLEKMMPYFSEIFANASSMHSQGQKAA
                     EAIEEARLQVAGLINASPSEIIFTSGGTESDNLAIRGLFLRLCPEKNEIITSAIEHHA
                     VLHTVQDLEKHYGAKAIYLPVDGKGRVNPADVEKAITGKTSLVSIMMANNEVGTIEPV
                     EEIARICKLRGVAFHTDAVQAVGKIPVDVKALGVDLLSMSAHKFYGPKGVGALYVKKG
                     VRLNPVMTGGSHERNLRSGTYNTPGIVGMGWAAKLAKDKLLWHMEYTAMLRDALQKRI
                     FDEIPDVFVNGDEKNRLPQLLNISIKFAEGESMLMFLDMDGNIQASSGSACTSKSLAA
                     SHVLNAMCIPHEFINGSIRFSFGKDNTVDHVDKVMAIFPQIVKKLREMSAIGPDKPAR
                     "
     gene            4910..5323
                     /gene="nifU"
                     /locus_tag="PK717_00130"
     CDS             4910..5323
                     /gene="nifU"
                     /locus_tag="PK717_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOR46114.1"
                     /translation="MYSEKVIEHFRNPRNVGVINDADGVGEVGNPVCGDMMTFYIKVK
                     DGIITDIKFKTFGCGAAIAVSSMISELVKGKNVDEALSISSKAVVEALGGLPSNKIHC
                     SNLGAVALARAINDYKRRNGLPYENIPEPEDVEEG"
     gene            complement(5334..5409)
                     /locus_tag="PK717_00135"
     tRNA            complement(5334..5409)
                     /locus_tag="PK717_00135"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5374..5376),aa:Phe,seq:gaa)
     gene            5511..6620
                     /locus_tag="PK717_00140"
     CDS             5511..6620
                     /locus_tag="PK717_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOR46115.1"
                     /translation="MAWRLLDCGGLDGWTIQSIYEAVAKEVGKGNSPNTLVNCHPATP
                     YVCIGVHQLLEKEVEEDFCKKNNVPIVRRQAGGGAVFLDPYQFFYHIIVKNDDPLVMG
                     DPVTVSERLLRPTVSTYKAFGCDASFKPVNDVIIGHRKASGNALANMHGASFVIGNVI
                     MDADVEMMAQVLKVPSEKFRDKIIENVREHMTSLKRELGKEFTYEEVQKKYLPVFETE
                     LGVKLEKGEMTEAEKKNLEEIFATTKTDEWLHRRTRGHEKLMDMSGKRTKVMAGLIIA
                     EADLKSDKLIRTTLSIKDDHIDDIVISGDFFMMPSDSLPNLENALKGKKVDREELLNA
                     VNSFFATGPKVFGVKPEDIVEVIFKARDNSVGVIG"
     gene            6849..7433
                     /locus_tag="PK717_00145"
     CDS             6849..7433
                     /locus_tag="PK717_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR46116.1"
                     /translation="MDRKGEIMEAAVAVISEKGFAKTRIADIVAQAGVAQGTFYLYFE
                     SKEALMVEMAKELIERMQYSFQYDLAGQKTISQKEFIEKVYEIFHGFTGIVRDNAQTI
                     RILANEAKNNEELHRFQIMVLGRVSNYLTELLNLGKKSGILRENDYKSIISMALMAIL
                     QFFTSNAGYEFPMDNAIDEFIDICLYGLLKEKNA"
     gene            7426..9465
                     /locus_tag="PK717_00150"
     CDS             7426..9465
                     /locus_tag="PK717_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOR46117.1"
                     /translation="MLEKIGTWIVKNRIGVAISCLGILLLSAIGIGKLSINYDFYSYL
                     PQDINSVIGQKILQEDFGFSDNIYVVLSGKTVSETKEIIDRLNKMPDVAETFWYSDLE
                     DVKIPEDFASKEARDQFLSGEDTLIQITLAPTGRKPSQQAEAIKAELGVEARVVGDFL
                     YNQEVERLSNSAKSSMLVIAVIAILLVLVLALTQPAYSILFLVSAGIAILINFGLTGF
                     FRGQMSFMTSSIAAALQLAVTMDYAIFLLHRYEEEKKTLPSLEAMAKSIAKTSTAVLS
                     SGLTTMAGFLALLFMSMKMGGDMGIVMAQGVFVGFIVTLTILPALILIIEKPLNALKH
                     RNLFPNMEKLGNFVVKNKGIIALVFLVLAVPAFIGSNMQPVTYSFEEGIKFDESVQKD
                     LDLVMEKFGGGHTVSVVLLGMDENQRLEVEEKLSKVPHVKEIIGPLSTGSAYVPRSYV
                     PKKVLAKLESEGKHLLTVTLNAPDKTELEKSFENIKAIEKEYNGNVLATGADMIQYDI
                     EKMGVSDNLKVNIATILGVFAIVWIALRRFRPSLVLIVGIEVAIWLNIAIQWFLNMPP
                     TFYFGPVALGAIQLGATVDYSILIATRYFDELKDKSPEEAMKISIREGSPAIITSALT
                     LFGACAAISATSQISMVKDLTMLLARGALISGTIVIIAIPALLLIFKGKTRRKNA"
     gene            9458..11533
                     /locus_tag="PK717_00155"
     CDS             9458..11533
                     /locus_tag="PK717_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46118.1"
                     /translation="MRKVLLIAITASLALAVPVMAVSTPATVKRTQTVYTVLDWNGKP
                     VEESVVNWLRVEGTGEMIVQDKPEISDVISLDPGMKPEIAGRNVTWKFSSNGQKDIFY
                     TGKTDKPLPISFDVELLLDGKKTKPEQTIGKNGKITLSMSFTNKLPKTVNLRWKEGDK
                     TVTKEKTLYQPMTVMVQAEIPVDSYSSIEAKGAMSMTIGSRKRIIWTLLPSPDGKAVL
                     EYSSDSVVLPSVQVSILPRIPDISIPSIDPAIQDLLASVADNTDLLDMAGVEINLDPD
                     KILSQLVSLQSLLDASKQAIDDSKKQVSDLTKSFSDLEKGLKELASGADNLSQLSKGH
                     KQVVETMKQGLDSNTSGLTESVSVLQNSANSANKSSRELFVSKTTLDDTLSSVSKLKS
                     QTSDKDLQDKLSDIENSLKIIGQKIDSAKQESDKASQNLKVLVDGGTIGGKDIPSISS
                     MPENLKLLDQALTALISGGEVMSVQMPGINTTIDGLNAISSGLNTIIKGGDVGGTKTP
                     PLSEIPALITKNLSQFDVLVSGGTFHGVKVPNQKEILSMIDDFKKMAEDFGPQSDRLL
                     ELGKKLQQAIERNGGKEKVQKAMDESKQLLFGGQAELDKMREMEQNYKSFVGETPGAE
                     SSVLFVAKLDEVTIEKTKENNVKAQNTTPFYEDINKYKYYILIAAIAIIIASIFINMA
                     YKRKHKEQS"
     gene            complement(11646..14249)
                     /locus_tag="PK717_00160"
     CDS             complement(11646..14249)
                     /locus_tag="PK717_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46119.1"
                     /translation="MKKIALFLALFLSLVPFIAMAQILHWPMLGGNPLHEGEASAIPQ
                     SPLEQTWRKSLGIPTYSAPLTDGSKIYLSCGTRIDDSGAFFCLDPQDGRVVFRHDFAK
                     GMPQTPVLSGGRIIGACADGHIYGRDISGSEVYSTLIEGKGSRSSGVDNATMVIFGTT
                     GSKNNLVCVDAYTGNTNWTAAIASDIQTAPVAYGGLIYVLADDNNLYAVNDSNGNIEW
                     KWEVGSSPSAAPMIFDNHLLIPSSSGISSLHLAFEYKDLKVSWTRDDIGKVTTMPCTD
                     GINIFFGTDSGKLYCLNGANGNQDWEYNASSRVTCSPVVASGKVVFGCEDGNVTILDS
                     ENGKFVEKIALGAIPTAQPLVFWDRIIVSCSTGEVVCLGPKGEGGGGGEVVVDPEPKT
                     PILKIDCPQSAFVGQKVKIPILIEDAQNITGLKFTFAFDQSSIKYTGYEPGQFFSHDG
                     SNPETTSQEGDGEVVFGIGPAYPASGKGMILELEIEPLVPGKHGLTLIDATATAEGDL
                     TCEPLIVSDTLEVKLPRPEVEVLLDPALIDHGKITGKTSFKLRLTQKNGELADFIARG
                     SSEACMVFPDSGTIGGNNGIDIIATVDPAKLPKGPSEVVVSVNIIGKTLKSVIKVIVP
                     EEELPGTPPPCIKVDPIALDFGFIPRGREVSIDFTLTFDTDKEISGVIKTDKRWLRVT
                     PATFKTKGGEVTGIATIAASELPGVSSVLGYMTISAQGKICQDVRVEARVETQPSIVL
                     ELDVGVKRASIGSIKVDLDQAPRIRNERTLVPIRFVSESFGCKVQWEATTKKVTINRF
                     DDTIILWIGKKEAIVNGKMVSLDVAPSIEEGGTLVPIRFISQAFGASVEWFHETKHIR
                     ITYTPPDDYVF"
     gene            14351..14425
                     /locus_tag="PK717_00165"
     tRNA            14351..14425
                     /locus_tag="PK717_00165"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14383..14385,aa:Gln,seq:ttg)
     gene            14439..15785
                     /gene="glmU"
                     /locus_tag="PK717_00170"
     CDS             14439..15785
                     /gene="glmU"
                     /locus_tag="PK717_00170"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOR46120.1"
                     /translation="MEISALILAAGAGVRMKSKTPKVLHQILGRPMISYVLDCVRSLG
                     IEKIGVVVGVGAKEVMEELGDSYSYIPQPSQLGTGDAVIKARTTFEGQSGYLFVLCGD
                     TPLLRPATLKSMIDLAEAEKPSCVMLTAKVPDPTGYGRIIRDNWGAIKDIVEERDAKP
                     EEKMIAEVNAGVYLFKMEDLWPALDKITPDNDQKEYYLTDVIEILYQQEKTNVSVRST
                     DLDEYRGINNRWQLAEITGNLLIKRLKDLAMSGVTIIDPGSTYIETSVEIAPDVIIYP
                     QTYLAGKMSIGEGCEIGPFVFLNGKKDGITIGDNVKVGPFVRMRPLVEVEDGATIGCF
                     IDLKKSKIGKGSKVPHLTYLGDCEVGEKTNIGAGTITCNYDGTNKSKTKIGKGVFIGS
                     DTMLVAPVEIGDGAYTGAGSVITKNVPEDSLAIERSPMVIKEGWAKKKKEQNGEGK"
     gene            15782..>16409
                     /gene="prs"
                     /locus_tag="PK717_00175"
     CDS             15782..>16409
                     /gene="prs"
                     /locus_tag="PK717_00175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOR46121.1"
                     /translation="MIKIFSGSANEMLANEVCEHVDVPLSKMNVMKFADGEIKCWPGE
                     NVRGYDVFVIQPTNPPADNLMELLIMIDALKRASANSVNVIIPYYGYARQDRKSQARE
                     PITAKLVADILTVAGATRTMTIDLHANQIQGFFDIPFDNLTAMMIFKDYFESIGIRNP
                     VAVSDIGGVTRANLFANSMSGTVAVFAKTRPKPDVVESLYFIGDVKDKD"
BASE COUNT         4829 a         3778 c         3810 g         3992 t
ORIGIN      
        1 ccggcaatct gaaagaaccg ccaagctcaa tcagttcgcc cctggaaaca attacctcct
       61 tgccttcagc atgcgtcctg agaagaagga gtattgcggc cgcattgttg ttgaccacaa
      121 cggtagattc agcgccaaga agcattttga tgaccgattt cacatggacg cctcttttac
      181 ccctcttccc ttcggcgagg tcatattcga gattacagta gccctcaagc aggggggcaa
      241 ggtacagatt gggaagcatt ggggcccttc caaggttcgt atgcaaaaca atgccggttg
      301 catttatcac tcttttcaat gaccttttgg tcaatgggga gagtctcttt ttaagtgtgg
      361 tctcaacgag ttttttgacc tgtgattcat ccagtgcctc gccaccagca attttgtccc
      421 tgacagaatc gagggcctcc ctgataaggc tggaggttat tctctggtca aaatgtccat
      481 attctttttt gaaatcttca tcctcgagta ttgaagaaac ctggggaatc agcctaaaca
      541 gttctttcat ccggacctcc ctttgcaatt gctagcaaaa gttgagtttc tggtttatgc
      601 ccactctaat aaaacggcct gttccttttg caatcaggtt gccgtcctgg tcagttatat
      661 tggccgaagc ctcaatcagt ctccctttca gaccatcaat tttcccatgg acaacaaatg
      721 gcttgccaac aaacatcggc tttacaaatc tcacagtcat ttcggccgtt accacagagc
      781 cgccgtccag cctgaaaatg gcatgtgaca tcgcctcgtc tataagtgtg gacactatgc
      841 caccatgcac aatattgtcc cagccctcaa aatccttgtc tggcacaaac cttgcctcca
      901 catggtcatc aatcagtgca aactttagct tcaaacccct ctggttggat gttccacaag
      961 caaaacagtt cgaatcagta cctttgtatt cgggttccat caacactcca tcagttcttt
     1021 gtatatacag cgatcatata tcacttctat gccgttttct tcaaggattt tgaacgagtc
     1081 aggggcatat atcccgagct gaaaccacac aacccccggt ctgacagcaa gaaaatcgtc
     1141 caccattcca ggtattgcct ccgatctcct gaagacatcg attatatcta ttttttcttt
     1201 tatatctgaa agactggcca gaaatttgac gcccgaaatt tcttcgccag catatttcgg
     1261 gttgacagga taaacaccaa atcccctggt aataaggtac ttggttatgg gaaagcttgc
     1321 agaacactca tcaagagagg caccaacaac tgcaatgacc tttgccttgc caagaatgtc
     1381 cttgattcta tccatcttgc tcgagtatag ccttaatacg gggttggtca acgaaccttg
     1441 aaaaatggcc aatctggtct atttttggca gtgatggaaa ttcttggtcg aataaaaggg
     1501 ccgggtgacc tcaagaaatt gaggaccagg gaccttggca agctcgcttg cgaaataagg
     1561 gaagcggttg tcgacgccgt ttcaaaaaat ggtggccatc tggcatctaa cctcggaatc
     1621 gtggagttga caattgccct tcacaggatt tttgattccc ccaaagacag gataatcttt
     1681 gacgtgggcc accagtgcta cgcccacaaa attataaccg gaaggctgga caggtttggc
     1741 agcataagaa aaaaaggtgg gctttcgggt tttccaaatc ctgacgaatc ccaatatgac
     1801 ctttacaaaa cagggcacgc gtcaacggcc gtctctcttg cagccggaga ggcaattgca
     1861 agggacctca agggcgaaaa acattcagta attgcaattg ttggcgacgg atcacttacc
     1921 ggtggtgaat gcttcgaggc gctcaatcac ataggccaga tggacacaga tgtccttgtc
     1981 atcctcaatg acaaccagat gtcaatctcg ccatcggttg gtgccctctc gctgttcaca
     2041 agcaaactca gggcggcaat cttttatcgc aggttcagtc actatgtcgg caaaggtttt
     2101 tcaagaatgg gacgctttgg cgaatgggcc gtcgggatgg gcttcaggat aaaaggagcc
     2161 ctcaagagat ttcttatggc cgaccagtac tttgaacagc tcggattcag gtaccttggc
     2221 ccggttgacg gacacgacat accactcctg cttgcttttc ttggcaggat aaaacacatg
     2281 aaaggcccgg tactcctcca cgttgtcaca aaaaagggca agggctgttc atttgcagaa
     2341 tcaaaaccaa ccgaatacca tggcacggca ccattcaagc ctgcaaatgg tgaaacggca
     2401 aacggaaaac ccacatactc gaaggttttt ggggagtgca tggtcgaggc tgccaaaaaa
     2461 gacaaatcga ttgtggcaat aactgccgca atgtcggatg gaaccggcct tgcggaattc
     2521 cagagggtat ttccaggcag gctctttgat gtcggaatag ctgaacagca cgctgtgtct
     2581 ctggcttcag cctttgccag gaacgggctg aagcctgttg tggcaatata tagcactttc
     2641 ctgcagaggg cctacgacca gataatccat gatgtggcgc tcatgaatct gccggtcatt
     2701 ttcgcaatcg acagggcagg ccttgttcca gatgatggcc caacacacca gggcattttt
     2761 gatctggcct acctctcgct ggttccaaac atcaccgtca tggcgccatc ctgtgccgag
     2821 gaattgtgcc agatgctcga aattgctttc tcgtcaaagg gcccttcggc catcaggtac
     2881 ccaaaggatg tagccctaaa aaccgattgc gaagacgtgg ttttcggaaa agccgaactc
     2941 acaaagaatg gcgatgatat ccttcttgca tacatcggca ctttcaggca agaggtggcc
     3001 accatctccg agatgctcga aaaagatggc ttctcgtgcg ctgttttaaa catgaggttc
     3061 gccagtcctc tcgacacgga ttctatgtta aagcatgcaa aaggcaaaaa agttgtattc
     3121 ttcattgaag aaggcgtcac tggtggtgga atagggcaaa gattgatgga acatatacca
     3181 ccattgcgca taatcggcat ccaaaatgca tttccagaaa atggctccag aaaagaattg
     3241 cttgagtcac aaaaacttga cacaacctca atctacgaaa aaatcaaagc gtctctttca
     3301 aaatagttct gttctctgct aagaaaaaac gtcccagaca aatttttctt ggccacaaaa
     3361 acccaaaacc ttataatcat atttacaggt tacttgaaaa ttaacattga ggatatatta
     3421 tatggttcgt gatagaagac acgaaaccag taatgacagt tggagcggtg gccgatgttc
     3481 ttggggtctg tgagcagacc ctcaggcttt gggaacaaaa aggactcata accccgaaca
     3541 gggtcaacgg aagaaggctt tattccgcca gggaccttga acgtctcgaa ctcgtcaagt
     3601 tcctcatcaa tgaaaaaaag atgaacattc ctggtgtaaa aaaatttctg gagatatttg
     3661 ataactgcaa acaaaaaaat tgcgaagatg ttgatgaatg cctcaaatta tgggacaagg
     3721 aggggaaatg aactccattt atcttgacca ttctgccaca acaccgcttg acggggaagt
     3781 cctggaaaag atgatgccct atttttccga gatttttgcc aatgcctcct caatgcactc
     3841 ccagggccaa aaagcggccg aggcaataga agaggcaagg ttgcaagtgg ccgggctcat
     3901 caatgcaagc ccaagcgaga taatctttac atcaggtggt actgaatccg acaacttggc
     3961 aatcaggggc ttgtttttga ggctttgccc tgagaaaaat gaaataataa catctgcaat
     4021 cgagcaccat gcagttttgc acacggtgca ggaccttgaa aaacactacg gtgcaaaagc
     4081 tatatacctt cctgttgatg gaaagggcag ggtcaacccg gcagacgtcg aaaaagcgat
     4141 aactggcaag acatccctgg tttcaatcat gatggcaaac aatgaagtcg ggacaatcga
     4201 acctgttgag gaaatagcca ggatatgcaa gctgaggggt gtggcattcc atacggacgc
     4261 cgtgcaggcg gtagggaaga tccctgttga cgtcaaggcg cttggtgttg atttgctctc
     4321 aatgtcggcc cacaagtttt atggtccgaa aggtgttggt gctctgtatg tcaaaaaagg
     4381 tgtcagacta aaccctgtaa tgaccggtgg ttctcacgaa agaaacctcc gttccggaac
     4441 atataacacc cctggaattg tggggatggg gtgggccgca aaacttgcaa aagacaaact
     4501 cctctggcac atggaataca ccgcaatgct tcgggatgct ctccagaaaa gaatctttga
     4561 tgaaatcccg gacgtatttg taaacggcga cgaaaaaaac agattacctc agttgctcaa
     4621 catcagcatc aaatttgcag agggtgaatc aatgcttatg tttcttgata tggacgggaa
     4681 catacaggcc tcttccgggt cggcatgcac gtcaaaatca cttgccgcat cgcatgtgct
     4741 caatgccatg tgcatcccgc acgagtttat aaatggatca ataaggtttt cgtttggaaa
     4801 agacaacacg gtggatcatg tggacaaggt catggcgatc ttcccgcaga ttgtaaaaaa
     4861 actaagagaa atgtcggcaa ttggccccga caaaccagcg aggtgattca tgtattcaga
     4921 gaaggttatc gaacatttta gaaacccgag aaatgttgga gtgatcaatg atgcagacgg
     4981 tgttggcgaa gttggaaacc cggtctgcgg cgacatgatg acgttctaca tcaaggtaaa
     5041 agacggcatc ataaccgaca tcaagttcaa gacgtttgga tgcggagcgg caatagctgt
     5101 ttcgtcaatg atttccgagt tggtgaaagg gaaaaatgtt gacgaggcgc tctccatctc
     5161 cagcaaagca gtcgtggaag cgcttggtgg tttgccatca aacaaaatcc attgttcaaa
     5221 tcttggcgca gtcgcacttg caagggcaat aaacgactac aagaggagaa atgggcttcc
     5281 atacgagaac atcccagaac cagaggacgt tgaagagggc tagaaaataa aaatggtgcc
     5341 cagggacgga cttgaaccgc cgacacgagg attttcagtc cactgctcta ccaactgagc
     5401 tacctaggca cctcgcttga taaatttata tagataggcg cttttgtcaa gaaagtaaac
     5461 cagcagtcgt tgattcaaaa taaatctatt ttgtgaaaca ggagggtttt atggcttgga
     5521 gattgctaga ttgtggcggt ctggatggat ggacaatcca gagcatctac gaagcggtgg
     5581 caaaagaggt tggaaagggc aattcgccaa atacgctggt caactgccac ccggcaacgc
     5641 cttatgtgtg catcggcgtc caccagttgc tggaaaagga ggttgaagaa gatttttgca
     5701 aaaagaacaa tgtccccatt gtcagaaggc aggcaggtgg tggtgcagtt ttcctcgacc
     5761 cataccagtt cttctaccac atcattgtca aaaatgatga cccgctcgtc atgggtgatc
     5821 ccgtcactgt ttcagagaga cttctcaggc caacggtttc tacatacaag gcttttggct
     5881 gtgatgcgtc attcaagccg gtaaatgatg taattatagg ccacagaaag gcctctggga
     5941 acgccctggc gaacatgcat ggggcctcgt ttgtaatcgg aaatgtaatc atggatgcgg
     6001 acgtcgagat gatggcccag gtactgaaag tcccgtctga aaaattcagg gacaaaataa
     6061 tcgaaaacgt cagggaacac atgacttcgc tgaagaggga gcttggaaaa gaattcacct
     6121 acgaagaagt ccagaaaaag tatctgccag tctttgaaac cgaactgggc gtcaagcttg
     6181 aaaaaggcga gatgaccgaa gcggaaaaga aaaaccttga agagatcttt gccacaacaa
     6241 aaacagatga atggctccac cgcaggacaa ggggccacga aaagctcatg gacatgtctg
     6301 gcaagaggac aaaagtcatg gccgggctta tcatcgccga ggctgacctc aaatctgaca
     6361 aactgatcag gaccaccctc tcaatcaagg atgaccacat agacgacatc gtcatctccg
     6421 gcgatttctt catgatgcct tcagactcac ttccaaacct tgaaaacgcg ctcaagggca
     6481 aaaaagtgga cagggaagag cttttgaatg ccgtaaacag tttttttgcc acagggccaa
     6541 aagtgtttgg cgtcaagccg gaggacattg tcgaggtcat ctttaaggcc agggacaatt
     6601 ctgtcggcgt tataggctag cctagcctat aatcattgat tctaaagcaa gctggtggca
     6661 tttttaattt gccaccagct ttttattcgc ctgaattttc aattcaaaaa cagctattct
     6721 tcgcagttta ttcaacgact ttcaatcggt taagttcttt tgcatatctg ccttgactca
     6781 aaatcttcat tgtgatatat gactgattag tcagtcactg actgatggtt cagtcacaag
     6841 gagatgagat ggacagaaaa ggcgagataa tggaagcggc agtcgcagtc atttctgaaa
     6901 agggttttgc caaaaccagg attgcagaca tcgttgccca ggctggagtg gcacaaggga
     6961 ctttctacct ctatttcgag agcaaggaag cgctgatggt ggaaatggca aaagagctca
     7021 tcgaaagaat gcaatacagc ttccagtatg accttgctgg acaaaaaaca atctcacaaa
     7081 aagagtttat tgaaaaggta tatgaaatat tccacggttt tacagggatc gtgcgcgata
     7141 acgcccagac aataaggatc cttgcaaacg aggccaaaaa caacgaggag ctccacaggt
     7201 tccagataat ggtgctggga cgggtaagca actacctgac cgaactcctg aatttgggga
     7261 aaaaatcagg catcctgagg gaaaacgact acaagtcaat catcagcatg gctttgatgg
     7321 caatcctgca gttcttcact tccaatgcag gctacgagtt cccaatggac aatgctatcg
     7381 atgaattcat tgatatttgc ctttacggcc ttctgaagga gaagaatgct tgagaagatc
     7441 ggaacttgga tcgtaaagaa caggataggc gttgcgattt cctgccttgg tatccttctg
     7501 ctctccgcca tagggattgg caagctctca atcaattatg atttttactc ataccttccc
     7561 caggacatca attctgtgat tggccaaaaa atattacagg aggattttgg gttttcggac
     7621 aacatctacg tggtgctttc cgggaaaaca gtcagcgaaa ccaaggagat catcgacagg
     7681 ctaaacaaaa tgccggatgt tgcagaaacc ttctggtatt cagatcttga agatgtgaaa
     7741 ataccggaag attttgcttc aaaagaggcc agggaccaat tcctttcagg cgaggacacg
     7801 ctcatacaaa tcacgctggc accaactgga agaaagccat cgcaacaggc tgaggccata
     7861 aaagcagaac ttggagttga agcaagggtt gttggtgatt ttctgtacaa tcaagaggtt
     7921 gaaaggctct ctaattcagc aaaaagctcg atgcttgtaa ttgctgtcat agcaattctc
     7981 ctggtacttg ttctggcact cactcagcca gcttattcga tactctttct tgtttctgca
     8041 ggaatcgcca tactcataaa ttttggcctg accggatttt tcagggggca gatgagcttc
     8101 atgacatcgt ctattgccgc tgcactccag cttgcggtaa caatggatta tgcgatattc
     8161 ctgctacacc gatacgagga ggaaaagaaa acgctcccct ccctggaggc aatggcaaaa
     8221 tcaattgcaa aaacttccac agctgttctt tcctctggcc tgaccacaat ggcagggttc
     8281 ctggcgctcc tcttcatgag tatgaagatg gggggagaca tgggaatagt aatggcccag
     8341 ggcgtttttg tgggtttcat cgtgacactt acgatactgc ctgccctaat attgataatt
     8401 gaaaaaccgc tcaatgccct aaaacacaga aacctctttc caaacatgga aaagcttggc
     8461 aatttcgttg tcaaaaacaa agggataata gcacttgtct ttttggtgct tgcagtgcca
     8521 gctttcatag gctcgaacat gcaacctgtc acttactcct ttgaagaggg aatcaaattt
     8581 gacgaaagtg tgcaaaaaga ccttgatttg gtgatggaaa aattcggtgg cggccacaca
     8641 gtctcggttg ttttgcttgg aatggatgaa aaccagcgac tggaggttga ggaaaaacta
     8701 tcaaaagtgc cccacgtcaa ggaaataatc ggcccacttt caaccggctc tgcctatgtt
     8761 ccaagatcgt acgtgcccaa aaaagtgctt gcgaaactcg agtccgaagg caaacatctg
     8821 ctgactgtaa cactgaatgc accggacaaa accgagctgg aaaaatcctt tgagaacata
     8881 aaagcaattg aaaaggaata taacggcaac gtactggcaa caggtgcgga catgatccaa
     8941 tacgacattg aaaaaatggg tgtatcagac aacctgaaag tcaatattgc aaccatcctt
     9001 ggggtattcg caatagtctg gatagcactc aggagattta ggccatcact cgttctaata
     9061 gtcggaatag aggtggcgat atggctcaac atagcaatcc agtggttctt gaacatgcca
     9121 ccaacattct actttggtcc ggttgcgctt ggagcaatac aacttggcgc aacagttgac
     9181 tattcaattc taatcgcaac aagatatttc gatgaattaa aagacaaatc gccagaagag
     9241 gccatgaaaa tatccataag agaaggctct ccggcaatta tcacatcggc actcacactt
     9301 tttggcgcat gtgccgcaat ttcagcaact tcacagattt caatggtcaa agacctgaca
     9361 atgctgttgg ccagaggagc gttgatttct ggaaccatag taataatcgc aatacctgca
     9421 ttacttttga tcttcaaagg aaagacgagg aggaaaaatg cgtaaggtcc tcttgatagc
     9481 aataactgca tcactggcac ttgcagtgcc ggtgatggct gtaagcacac cagcgacagt
     9541 aaaaagaacc caaacggtct atactgtgct ggactggaac ggaaaaccag ttgaagaatc
     9601 cgttgtaaac tggctcaggg tcgaaggaac gggtgaaatg atagtccagg acaagcctga
     9661 aatctcggat gtaatatcgc ttgatccagg catgaagcct gaaattgctg gccgcaatgt
     9721 aacatggaaa ttcagttcca atgggcaaaa agatatattt tacacaggca aaacagacaa
     9781 accactgcca ataagttttg atgtggaatt gttattggat ggcaaaaaaa caaagccaga
     9841 acaaacaatt gggaaaaatg gcaaaataac cctctccatg tccttcacaa ataaactgcc
     9901 aaaaactgtt aacctgcgct ggaaagaggg tgacaaaaca gtcaccaaag aaaaaaccct
     9961 ctaccaacca atgactgtga tggtgcaagc tgaaatacca gtggattctt atagctccat
    10021 cgaagcaaag ggtgccatgt cgatgaccat tggaagccgt aaaaggatca tctggacgct
    10081 ccttccaagc cctgatggta aagctgtgct cgaatattca agcgattcgg ttgtgttgcc
    10141 gtctgtccaa gtctcgatct tgccaagaat ccctgacatc tcgataccaa gtatcgaccc
    10201 tgcaattcag gaccttctgg catccgttgc cgacaatact gatcttctcg acatggccgg
    10261 agtagaaatc aaccttgatc cagataaaat attatcccag cttgtatcgc tccaatcgct
    10321 tcttgatgcc tcaaagcagg cgattgatga ctccaaaaaa caggtctctg acctgacaaa
    10381 atcgttctct gatcttgaaa aggggctcaa agagcttgcc agtggggcag acaacctctc
    10441 ccagctaagt aagggtcaca agcaggtcgt tgaaacaatg aagcaggggc ttgattccaa
    10501 caccagcggc ttgacagaga gtgtatccgt actccagaat tcagccaatt ctgcaaacaa
    10561 atcatccaga gagctgtttg tttccaaaac cacgcttgat gacacgttgt catcagtctc
    10621 aaaactgaaa tcccagacca gtgacaagga cctccaggac aaactttcag acattgaaaa
    10681 ctcgctcaag atcatcggtc aaaaaatcga ttctgcaaaa caggagtccg acaaggcatc
    10741 gcaaaacctg aaggtgctgg tggatggtgg aaccatcgga ggcaaggaca taccgtcaat
    10801 atcttccatg ccggaaaacc tcaaattgct cgatcaggcg ctaaccgcgc tcatttccgg
    10861 cggagaggtc atgagcgtcc agatgccagg aataaacaca accattgacg ggcttaacgc
    10921 aatatcttct ggcctgaaca caataatcaa gggtggagac gtaggcggga caaaaacacc
    10981 gccgctttca gaaataccgg ccctgataac aaaaaacctc tcacagtttg atgttctggt
    11041 ttcaggcggg acattccatg gggtcaaggt gccaaaccag aaagaaatcc tctcgatgat
    11101 tgatgatttc aagaaaatgg ctgaagactt tggcccgcaa tcagacaggt tgctggaact
    11161 cggaaagaaa ctccagcagg ccatcgagcg caatggcgga aaggaaaaag tccagaaggc
    11221 gatggacgag tcaaaacagc tcctgtttgg tggccaggca gaactggaca agatgaggga
    11281 gatggagcaa aactacaaat catttgtggg tgaaacacca ggtgccgaga gctctgtcct
    11341 ctttgtcgca aaactggacg aggtcaccat tgaaaaaact aaagaaaaca atgtaaaagc
    11401 gcaaaatacc actccattct atgaagatat aaataaatat aagtattaca tacttattgc
    11461 tgctattgct atcataatag catcaatatt tattaatatg gcatataaga ggaaacataa
    11521 agagcaaagt tgatttaatg taccaaaaat tatcatatta ttagattaga tatctaataa
    11581 tactttgaaa tagtaaacag gccccttcaa atttataaaa gggcctcttt gtatgccatg
    11641 cttatctaga acacataatc atccggcggg gtataggtga tcctgatgtg ctttgtctcg
    11701 tgaaaccact cgacacttgc gccaaacgcc tgggagataa accttatcgg tacaagggtc
    11761 ccaccttcct caattgaagg ggcaacatca aggctgacca ttttgccatt cacaattgcc
    11821 tcttttttgc ctatccagag gattatcgtg tcgtcaaagc ggtttatggt gacctttttt
    11881 gtggtcgctt cccactgtac cttgcaccca aaagactctg acacaaacct tattggaaca
    11941 agcgtcctct cgtttcttat acgcggcgcc tggtcaagat cgacctttat cgagccaatg
    12001 cttgccctct tcacgccaac atcaagctcc agcacaatgc ttggctgggt ctccaccctg
    12061 gcctccacgc ggacatcctg gcatattttc ccctgggccg aaatggtcat gtagccaagc
    12121 actgatgaga cgcctgggag ctccgaagca gctatggtgg ctattccggt aacctctcca
    12181 cccttcgttt tgaacgtggc cggcgttacc ctcagccacc tcttgtcggt tttgattaca
    12241 ccagatattt ccttgtcggt atcaaatgtg agcgtgaaat cgattgatac ctctctgccc
    12301 ctcgggataa agccaaaatc aagcgctatc gggtcaacct tgatgcaggg tggtggggtt
    12361 cctggcagtt cttcttccgg cacaatgact tttatgactg atttgagggt ctttccgatt
    12421 atgttcactg agacaacaac ctcgctgggc ccttttggca attttgccgg gtcaacagtg
    12481 gcaatgatgt ctattccatt gttcccgccg attgttcccg agtcaggaaa aaccatgcag
    12541 gcctccgatg agcctctggc aataaaatcg gccagttcac catttttttg cgtgagtctg
    12601 agcttgaaac tcgtctttcc ggttatcttc ccgtgatcaa tcaatgcggg atcaagaagg
    12661 acttccacct ccggtcgcgg cagcttgact tcaagagtgt cagaaacaat caggggctcg
    12721 caggtcaaat caccctcggc tgttgcagtg gcatcgatta gtgtgaggcc atgctttcct
    12781 ggaacaagtg gctcaatctc aagctccaga atcatgccct ttccacttgc gggataggct
    12841 ggaccaatcc caaaaacaac ctctccatcg ccctcctgcg atgtcgtttc aggattggat
    12901 ccatcatggg agaagaactg acctggttcg tatcctgtat acttgatgct tgactggtca
    12961 aaagcaaagg tgaatttgag cccggttatg ttttgtgcgt cttcaatgag tattggtatt
    13021 ttcacctttt gccctacaaa tgcagactgt gggcaatcaa ttttcagaat gggcgtcttt
    13081 ggttcggggt caacaaccac ttcgccacca ccgccacctt cgcccttcgg cccaaggcaa
    13141 acaacttcac ctgttgagca ggaaacgatt attctgtccc aaaaaaccag cggctgtgcc
    13201 gttggaatgg ctcccaaggc aattttttct acaaacttgc cattttcaga gtccaggata
    13261 gtgacgttgc catcctcaca cccaaaaaca accttgccag atgcgacaac aggcgagcag
    13321 gttactctcg aagaagcatt gtattcccag tcctggttgc catttgcgcc gttaagacaa
    13381 tagagcttgc ctgaatcagt cccaaagaaa atgtttattc catcagtaca tggcatggtt
    13441 gtcactttgc caatatcatc ccttgtccag gaaactttca aatctttgta ttcaaatgca
    13501 agatgcaaac ttgaaatacc cgatgaactt ggaatcagga ggtggttatc aaagatcatc
    13561 ggagcggcag atggagacga accaacctcc catttccatt caatatttcc attcgaatcg
    13621 ttaaccgcat aaaggttgtt gtcatcagca agaacatata tcaaaccacc gtaagcaact
    13681 ggagcagtct ggatatcaga ggctattgcc gcagtccagt tcgtgtttcc tgtatatgca
    13741 tcgacgcaga caagattgtt ttttgaacct gtcgttccaa aaattaccat ggttgcatta
    13801 tcaacgccgg acgatcttga acccttgcct tcgatgaggg tggaataaac ctcggagcca
    13861 ctgatgtctc tgccatatat gtgcccgtca gcacaggcac cgattatcct tccaccagac
    13921 aaaactgggg tttggggcat gcctttggca aaatcatgcc tgaaaaccac tcttccatct
    13981 tgtggatcaa ggcagaagaa agctcctgaa tcatcaattc ttgtgccaca agaaaggtat
    14041 attttgctgc catctgtaag cggagcgctg taagtcggga ttcccagtga ttttctccat
    14101 gtctgttcaa gcggagattg agggatagca gaagcttccc cttcatgaag ggggttccca
    14161 cccagcatcg gccagtgaag tatctgggcc atggcaataa agggtaccag gcttaaaaac
    14221 agtgccaaaa acaaggcaat ttttttcatc aaaatcctcc attggcaata cacttagctt
    14281 cagtcaatgg tttaactgtt gacatgcata ccatgaaggc taaaatttat ctgccgtatc
    14341 aaattttcgt tggggagtcg ccaagcggta aggcatcagc ctttggagct gacactcgca
    14401 ggttcgaatc ctgcctcccc agccaaaaat ggagacatat ggaaatctca gcactcattc
    14461 ttgcggcagg cgccggagtc aggatgaaat ccaagacacc caaggtgtta catcaaatcc
    14521 ttggaaggcc aatgatatcc tatgtgctcg attgcgtcag gtcgcttggc atagaaaaga
    14581 ttggcgtggt ggtaggggtt ggagcaaaag aggtaatgga ggaacttgga gattcgtatt
    14641 cctacattcc ccagccatcc cagctcggaa caggtgacgc tgtcataaaa gccagaacaa
    14701 cctttgaagg tcaaagcggc tatttgtttg ttctgtgcgg cgatacgcca ctccttaggc
    14761 cagcaacctt aaaatcaatg atagacctgg cggaggcgga gaaaccatcc tgtgtaatgc
    14821 tcactgcaaa ggtcccggac ccaaccggat acggaaggat cataagggac aactggggtg
    14881 ctataaaaga catagtcgag gaaagggacg ccaaacctga agaaaagatg attgccgaag
    14941 tgaatgcagg tgtttatctt ttcaaaatgg aagacctctg gccagccctc gacaaaatca
    15001 caccagacaa tgaccagaaa gaatattatc tgactgatgt tatagaaatc ctctaccagc
    15061 aggaaaaaac aaacgtaagc gtcaggtcca ccgatcttga tgaatacaga ggaatcaaca
    15121 atagatggca gcttgcagaa atcacaggca atcttttaat caaaaggtta aaagatttag
    15181 caatgtctgg agtcaccata attgatccag gctccacata cattgaaaca agcgtagaga
    15241 tagcaccaga tgtaataatc tacccccaaa cctacctggc tggcaaaatg agtattggag
    15301 aaggctgtga aatcggtccg ttcgtatttc tcaatggcaa aaaagatggc ataacaatag
    15361 gcgacaatgt caaagtaggg cctttcgtaa ggatgcgccc acttgtcgaa gttgaggatg
    15421 gtgcaacaat agggtgtttc atcgacctca aaaaatcaaa gataggcaaa ggatcaaagg
    15481 tccctcacct tacatatttg ggtgactgtg aagtaggtga aaaaacaaac attggcgcag
    15541 gaacaataac gtgcaattat gatggcacaa acaaatccaa aacaaagatt ggaaaaggcg
    15601 tctttatcgg ttcagataca atgctggtgg cgccagttga aataggcgat ggtgcataca
    15661 ctggcgccgg ttctgtaatc acaaaaaatg ttcctgaaga ttcacttgca attgaaagaa
    15721 gtcctatggt cataaaagaa ggttgggcca aaaagaaaaa agagcaaaat ggagagggaa
    15781 aatgataaag atattctctg gttctgccaa tgagatgctt gccaatgaag tttgcgaaca
    15841 tgttgatgtt cctctttcca aaatgaacgt aatgaagttc gcagacggtg aaataaagtg
    15901 ctggccagga gaaaatgtca gaggttacga tgtttttgtt attcaaccga caaacccgcc
    15961 agcagacaac ctaatggaac tgctgataat gattgatgcc ctgaagagag catcagcaaa
    16021 ctcggtcaat gtaattatcc cctactacgg ctatgcaaga caggacagga aatcacaggc
    16081 cagagagcca ataacagcca agctggttgc tgacatattg acagttgcag gtgccacaag
    16141 aacaatgacc attgatctgc atgcaaacca gatccaggga ttttttgata tcccatttga
    16201 caatctcact gcaatgatga tttttaaaga ttactttgaa agcattggca tcagaaaccc
    16261 cgttgcagtt tcagatattg gtggcgttac cagagcaaat ctttttgcaa acagcatgag
    16321 cggcacagta gcagtgtttg caaaaacacg cccgaaacca gatgttgttg aatcactcta
    16381 ctttatcggc gatgtaaaag acaaagatg
//
LOCUS       DAOSRI010000003        14757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4152_length_14757_cov_3.906679, whole genome shotgun sequence.
ACCESSION   DAOSRI010000003 DAOSRI010000000
VERSION     DAOSRI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_14757_cov_3.906679"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1121)
                     /locus_tag="PK717_00180"
     CDS             complement(135..1121)
                     /locus_tag="PK717_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOR46122.1"
                     /translation="MSSPIRKRKKRSPCLPWFLGIFAVGFILLFFVFNYFYGLLGQFN
                     SNQFVSDPGRPVASLNFSKRVNILIIGVDAPSLKESARSDVLMVASYYAKENNLKMLS
                     VPRDTMVNPQGLGVCKINACNNPAFNIKFGTPFLIKAVEELLGIKIDAYVKTNFRGFS
                     DIIDKLGGIDLNVEKDMLYYDPIDKFKIDLKKGYQHLNGSMALQYVRYRSDLADFVLV
                     DGKPMGRVARQANFMKAILKEASSPKNWFKLGQVMQAATDAIETDLTPSMMIRLATKM
                     KDVGPDGIKVLGFPGTDQYVNKISYVIPNKVEIEKLVSENFLAGSDPIKPNP"
     gene            complement(1118..2680)
                     /gene="murJ"
                     /locus_tag="PK717_00185"
     CDS             complement(1118..2680)
                     /gene="murJ"
                     /locus_tag="PK717_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOR46123.1"
                     /translation="MNTKKVIKDIGIVTVLLGLRSVVGFIRDVVMASRFALDYTMDAF
                     NIASKVPLTIANLLVMGVFTAVFLPIFTRYIIENKKEELNKLVGILFIIITVGFTVLI
                     ILAEIFARPFIMYVLTNNATAETVDEAIVLFRLMMPAVLLMAWSGLASGIHNSFQRFT
                     MPAVGNLLFNIWMVLPAWIFPQSWGIRGFAVGVCIGALFQLLVQLPGLFGKHMKLDIN
                     FNMRHPALMGIGSLVIPVLLSSSINYLVPFVETFLASGIANGAVSALAYAFRISQLPL
                     GIFVLAISSVVYPLFAEAVAKNNPEALKSNIQWGLKLVVMIIIPAGFGLLALSHPIVM
                     MLFQRGKFGIVDTVMTSAPLAFYGLAIIPMAIEAVLVKVFYSISDTKTPVKIYLSNLI
                     MLVILDVVFVKMGLGVVGLALGATICAFVRILMVVIAMRKKIGIFGLSGLMVSIIKST
                     VASIVMSIVAYLIAIRANYVVNLGSNFGRSLQVGFAIVVGVAVYAVVLYIIDRKEINQ
                     MLVVFKRRGQSA"
     gene            complement(2682..3188)
                     /locus_tag="PK717_00190"
     CDS             complement(2682..3188)
                     /locus_tag="PK717_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 family protein"
                     /protein_id="HOR46124.1"
                     /translation="MVILDEKGRLFGKLNIIDLLVLIVLLYGLYWGGTKIITKYFVHP
                     KFDSYVVVLRAENVNPEIIEGIHIGDKLVERSGTVIGAVIKPEPALKDSMVYVQTREG
                     LIVESVQPKLKDMDITVEVKTPEGTRVKYNTNNMLVGSRLEYDFVSEGMMGIHVKALC
                     VSINKAGN"
     gene            complement(3210..5588)
                     /locus_tag="PK717_00195"
     CDS             complement(3210..5588)
                     /locus_tag="PK717_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR46125.1"
                     /translation="MKFERYTDALKDVMSDAQQVLERYHDIELDNEHLFVALLEKEDS
                     VVREILSNLGKGWRDILQEVERTLASHPHPTQATTRSQIYITPRAKSTLERAEEEANS
                     LGDEFVGPEHLFIALSQDTRETGRIMTQRGLDRETILQGLAKIRGGEGGGSGQAKTAL
                     DKFTRDLTDLAKKGKLDPVIGRELEIERSLQVLSRRTKNNPVLIGEAGVGKTAIVEGI
                     AQRIVDSNVPDSMRGKKILSLDMGALVAGTKFRGEFEERLKSVIDEVKKAEGKIILFI
                     DELHLVMGAGAAEGAMDASNMLKPALARGELHCIGATTTDEYTKYIEKDSALERRFQP
                     VNVPEPTVAQTVEILRGLRDRYEAHHGVKISDEALATAAELSSRYITNRFLPDKAIDL
                     IDETAARIRVTMESMPPKLASLKSETERLQREGEAAAKSGDTNRAMKLKTEYEKCNAE
                     YISEREKWLREVKVSEEVGPEEIAQTISRWTGIPVSSLTESEQQKLIKLEERLHERVI
                     GQDEAVTAVAQAVRRSRAGLSDPNRPIGSFLFLGPTGVGKTELAKSLAELLFDSDQAL
                     TRFDMSEYMEKHTVARLIGAPPGYVGFEQGGQLTEAVRHRPYQVLLFDEVEKAHQDIF
                     NVFLQLLDDGRLTDGQGRTVDFRNCIIIMTSNLGSQMVEPDMPYGKMKEVYMEAVRKN
                     FRPEFVNRLDGIIVFKHLTREELGKIVSIQISRVQKMLDKKGIKLDVTKEAQELLGEI
                     GYSVEYGARPLKRTIQQELENRLADLILTGKLKDGKTARVEVSDGALAIKEI"
     gene            complement(5818..6210)
                     /locus_tag="PK717_00200"
     CDS             complement(5818..6210)
                     /locus_tag="PK717_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46126.1"
                     /translation="MNKLTLAWLALAVLGGAAFGLVGWLVVGISSPPFSSYILSGLVV
                     LGLLPGIFGAFGLRFVGLVGIVGIPLTLLWAIIDAFFFHGGYIWPFFFMMNMAQGIVL
                     SIGIGCLAEFFLCFRKPKKRRNYAYGVD"
     gene            complement(6203..8116)
                     /gene="thrS"
                     /locus_tag="PK717_00205"
     CDS             complement(6203..8116)
                     /gene="thrS"
                     /locus_tag="PK717_00205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOR46127.1"
                     /translation="MISVNCSGKSAQIEKGSNIFQALKALEIPTKNLVAAKIGDKLAD
                     LSTMLEENTNFEVIAIDSPEGRDILLHSSAHLMAAAVNRLFPGTKFAIGPSIKDGFYY
                     DLDLPKPLVEEDLPKIEAEMKKISDENIKFVRKTMTKEDALAFFGARGDTYKVELIQD
                     IPDPTVSIYELGDFIDLCRGPHLPSSGYIKHFKLMSIAGAYWRGDEKRQMLTRIYGTS
                     YSTKDGLEEHIKRLEEIAKRDHRILGKQLELFIIDEHVGGGLVIWQPNGAVVREIIEN
                     FWRTEHRKRGYEIVYSPHVGKADLWRTSGHLGFYADNMYPAMELETVEYYARPMNCPF
                     HMVNYASKVRSYRDLPYRTAELGTVYRFERSGTLHGLLRVRGFTQDDAHIFCTPEQLK
                     QEVVGVVKFAIHLLETFGFKKYEVFLSTRPKEGYVGEIEKWDIAESTLKEALNEMGLS
                     FDVDEGGGAFYGPKIDIKIVDALGRPWQCTTCQFDFNNPERFNLTYTGEDGKEHRPYT
                     IHRALLGSMERFMGCLIEHYAGAFPVWLAPTQVVIVPITDGVLNYCKSLKDRLFDADI
                     RVKIDEGSDRMNAKIRNAELKKVPYVVVVGAKEAEAGKVSVRRHGKGDLGQMTTEEFE
                     ARILAEVKERSIE"
     gene            complement(8125..8781)
                     /locus_tag="PK717_00210"
     CDS             complement(8125..8781)
                     /locus_tag="PK717_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOR46128.1"
                     /translation="MIQLDRIENDPYIVELLKASDRQIAALGYTEHGIRHATVTCEAA
                     RKICSGLGLSQWETDVACAASFLHDIGNLTGRENHGPLGATLVSPFLVQHGLLPEELA
                     RVITAISCHDDDEKPVISTVTTAVTIIADKSDVNRSRVRPTADIKTDIHDRVNYAVLE
                     SNVSASKGKVLLSLRLDPQIACTADYLEIFASRMQAMRKSAQFLGCEYELEINNVKIF
                     "
     gene            complement(8759..9877)
                     /gene="tgt"
                     /locus_tag="PK717_00215"
     CDS             complement(8759..9877)
                     /gene="tgt"
                     /locus_tag="PK717_00215"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR46129.1"
                     /translation="MSCYKLEASNGRARAGILKTAHGEIKTPVFMPVGTQATVKALTP
                     EQVKEIGFGLILCNTYHLMLQPGENLVHEAGGLHKFMNWDGSILTDSGGFQVMSQASL
                     REITREGVHFKSYLDGSAHFLTPESAIGIQEKLGSDIAMVLDICTPYPIAQQKARHYT
                     EITHEWAKRSLEARSKPDQLVFGITQGGFELTDRAWSCDAITSMDFDGFAVGSLSVGE
                     PLELAGEILDVVVPRMPKDRPRYLMGVGDPAGIIQGITSGIDMFDCVLPTRLGRNSAV
                     MTKEGTLSISKSRFAKDFRPIEEGCDCYTCRNYTRAYLRHLFKAKEMLAATLASIHNL
                     HFLKRVVDGARNAIIDGTFDQYASKWLNWREDDSTGQD"
     gene            complement(9874..11106)
                     /locus_tag="PK717_00220"
     CDS             complement(9874..11106)
                     /locus_tag="PK717_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOR46130.1"
                     /translation="MSIFDEESLKLHHLHKGKIRVDVKDDLRSKHALSVWYTPGVAEP
                     CRQIYRNPETVYDYTIKGNTIAVVTDGSRVLGLGNIGPEASIPVMEGKCALFVEYGAI
                     DAFPLPLKARTNEEFVAAVKAMEPIFGGINLEDIQIPHCFEIEEILIKELDIPVFHDD
                     QHGTAVVALAGIYNALKLLGRKIENQRVIISGAGAAGSAVAKLLVAAGIGDVILFDRE
                     GVICRGRTDYMMPYKYKLAEITNKENFKGTLHEGLVGADIFIGLACPGLVAGEDIKKM
                     AKDPIVFALSNPDPDISPEEAKSAGAKIICTGRSDLGNQVNNLLAFPGIMRGALDSRA
                     RVINEAMKVAASMTIANLLPENELREDRIIIEPFAKGVAQAVAKSVAKAAFDTSVART
                     NLTLQEVCDLIERRLEDK"
     gene            11327..11857
                     /gene="infC"
                     /locus_tag="PK717_00225"
     CDS             11327..11857
                     /gene="infC"
                     /locus_tag="PK717_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOR46131.1"
                     /translation="MEKKLRTNKEIRAREVRMIDDEGKQVGIVDIRKALEMAVLKGLD
                     LVEISPNSDPPVCKLLDYGKYKYELEKKVKENRRKQSSVEIKEMQFSLSVDENDYQTK
                     VGHVRRFLLNGHRTKVVIRFRGREMMHKDQGRALLDRIANEVASLGTIEQMPKMDGRH
                     MHMMIAPTKQKEKEIK"
     gene            11870..12112
                     /locus_tag="PK717_00230"
     CDS             11870..12112
                     /locus_tag="PK717_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOR46132.1"
                     /translation="MPKLKIRSKGSAKKRFRVTGTGKLRSQSPGFAHLLENKNRGETK
                     RKMRLKDVDKTMIRLMKCLLPVGVKGRKIRNEKKED"
     gene            12116..12472
                     /gene="rplT"
                     /locus_tag="PK717_00235"
     CDS             12116..12472
                     /gene="rplT"
                     /locus_tag="PK717_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOR46133.1"
                     /translation="MRVKGGIVARRRRKKVLNRASGFFGAAHRRFKIAKEQVLHAERY
                     EFIHRRQRKRDFRRLWITRINAAARMNGITYSRLVAGLNGAGFEIDRKILAKLAIEDA
                     NAFTKLVELAKANLKN"
     gene            12473..13789
                     /gene="glmM"
                     /locus_tag="PK717_00240"
     CDS             12473..13789
                     /gene="glmM"
                     /locus_tag="PK717_00240"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR46134.1"
                     /translation="MPRLFGTDGVRGIAGDELNEELASRLAFAASQQFFTKDGKVLVG
                     KDTRLSGDMIESALASGFVSGGCDVISVGIIPTPGLARLCKETGHFGAVISASHNPIE
                     YNGIKFFSPQGVKLRDEEEDEIEARMSQKPNGHTRGTFTKNINLCERYCQDIASFFNL
                     DMGGRKIVLDCAYGATYAVAPKLFKRLGADVIAINAEPDGSRINVDCGSTMPHMMCKT
                     VLEQKAEMGFAFDGDGDRCIACDSQGRIVDGDKMMGILAKYMVKKNRLPGMAIVTTVM
                     TNLGLEEHLSSCGIKMERTKVGDRYVHQRMTEKGLNLGGEQSGHIIIADRTTTGDGTL
                     SAISLVEALEETGASLSDELDLIPEYPQILLNVKVRDREEAMNSLLVAELTQKAQEKL
                     GKHGRILIRSSGTEALIRIMVEAVDEALCKEIAFDTTEKIKSRYGV"
     gene            13841..>14757
                     /locus_tag="PK717_00245"
     CDS             13841..>14757
                     /locus_tag="PK717_00245"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOR46135.1"
                     /translation="MTKISLSQAKQTIIKCQLLDKPAGKGKAGALKALEHLGYVQIDT
                     ISVIERAHHHVFWSRCPDHKKEHLHELQAKDRKVFEYWGHAASFLPMSDFRYSIPVME
                     AHRQRDDVKTVLDTYKELSVQVLDRIRQEGALSSSDFETPEDFERGPWWGWKPAKQVL
                     EALFWTGELMVSHRKNFQRYYDLPERVIPSSTNLEKPDEGKLAKFRVMAALRTLGFAN
                     IQDICWMRRNKIAMKKALQEMIEEGEAVEVEIEGLDGTYYCLANNLSVGEEKNGLNCN
                     ILSPFDNAVIRRDRLGKIFDFEYSFECYVP"
BASE COUNT         3948 a         3629 c         3321 g         3859 t
ORIGIN      
        1 gcgaaagtcc ccttcccgtt atccagaaaa taaacaatga ccccgagcca agtctgtcaa
       61 aaaacccgac aagggcatca aaaacctttc tggtaacaga tttctcccag aaaccacaaa
      121 ttatacaaag acaattacgg gtttggcttg attggatcag agcctgcaag gaaattttct
      181 gaaacaagtt tttcaatctc caccttattt gggatgacat agcttatttt gttgacgtac
      241 tggtctgtac ccggaaaacc caaaaccttg attccatcag gcccaacatc tttcattttt
      301 gttgccagcc ttatcatcat ggacggggta agatcagtct caattgcatc agtggcggcc
      361 tgcatgactt ggccaagctt gaaccagttc tttggtgaag aggcctcttt aaggattgcc
      421 ttcataaagt ttgcctggcg ggcgaccctg cccattggtt tcccatcaac gaggacaaaa
      481 tcggcaagat cagatctata cctaacatac tggagcgcca tgcttccatt gaggtgttgg
      541 tatccctttt tcaggtcaat cttgaattta tcaattggat cgtaatagag catgtctttc
      601 tcgacattga gatctatgcc gccaagcttg tctattatgt cggaaaaccc tctgaaattc
      661 gtttttacat aagcatcaat ctttatgcca aggagttctt cgactgcttt aatgaggaat
      721 ggggtaccaa atttgatatt aaaagccgga ttgttgcaag catttatttt gcatactcca
      781 agcccttgtg gattgaccat tgtgtctctt ggaacagaga gcattttcag gttgttctct
      841 ttggcgtagt aagaggcaac catcaaaaca tctgaccttg cagattcctt gagtgagggc
      901 gcatcaacac cgattatcag gatgtttact ctcttgctaa aatttaggct ggccactgga
      961 cgcccaggat ccgaaacaaa ctggttggaa ttaaactggc cgagcagccc atagaagtag
     1021 ttgaaaacga agaacagcaa tataaaaccc accgcaaata ttccgagaaa ccatggaaga
     1081 cagggactcc ttttttttct ttttcttatg ggactgctca tgcagattgc ccccttcttt
     1141 taaaaacaac cagcatctga tttatctctt tgcggtcaat tatgtaaaga acaacagcat
     1201 aaactgccac cccaacaact attgcaaatc cgacctggag actcctgcca aaattggagc
     1261 caagatttac aacatagttt gccctgatag caatcaaata agccacaata ctcattacaa
     1321 ttgatgccac agttgatttg ataattgaca ccatgagccc agagaggcca aatatgccaa
     1381 tctttttccg cattgcgata actaccatca agatccttac aaaagcacat attgttgcac
     1441 ccaacgcaag cccgacaacg ccaagaccca ttttgacaaa tacaacgtca agtatcacaa
     1501 gcattatgag attgctcaaa taaattttta caggcgtctt cgtgtcgctt atggagtaaa
     1561 agacttttac aagtacagct tcaatggcca taggaattat cgcaagacca taaaaggcca
     1621 gaggagcaga agtcatcacg gtgtccacaa tgccaaattt tcctcgctga aaaagcatca
     1681 tgacaatggg gtgtgaaaga gcgagtaacc cgaagccagc tggaatgatt atcatcacaa
     1741 ccagctttaa accccattgg atattggatt taagcgcctc cggattgttt tttgctacgg
     1801 cctctgcaaa gaggggataa actaccgaag aaattgcaag cacaaatata ccaagaggta
     1861 gctggcttat cctgaaggca taagcaagtg ccgaaacagc gccatttgca attccagaag
     1921 ccaggaaggt ttcaacgaag ggcaccagat aattgattga agaactcaaa agtactggaa
     1981 tgacaagact ccctataccc ataagggctg ggtgcctcat gttgaagttt atgtccagct
     2041 tcatgtgttt cccaaataac cctggcaatt ggacaagaag ctggaacagg gcaccaatac
     2101 acacgccaac tgcaaatcct cttatacccc aggattgtgg gaatatccag gcaggaagga
     2161 ccatccagat gttaaaaagg aggtttccaa ctgccggcat tgtaaaacgc tggaaggagt
     2221 tgtggattcc agaagcaaga ccagaccagg ccatcaaaag aacagccggc atcatgagcc
     2281 tgaaaagcac tattgcctcg tcaacagttt cagcagttgc gttgttggtg aggacataca
     2341 taatgaaagg ccttgcgaaa atctcggcaa ggattatcaa cacggtgaaa ccaacggtga
     2401 taataataaa aagtatcccg acgagcttat tgagttcttc ttttttgttt tcaattatgt
     2461 atctggtaaa gatgggcaaa aaaactgcag tgaaaacacc cataaccaag aggtttgcaa
     2521 tcgtaagcgg aaccttggat gcgatattga aggcatccat ggtgtagtcc agggcaaacc
     2581 tggacgccat gacaacgtcc cttatgaaac ccactacaga acggaggcca agaagaacgg
     2641 ttacaatccc aatgtctttg attacttttt ttgtgttcac gctagttgcc agccttgttt
     2701 attgaaacac acagggcttt tacgtggata cccatcattc cctccgagac aaaatcatat
     2761 tcaagtctcg atccaacaag catgttgttt gtgttgtact ttactcttgt gccctctggg
     2821 gttttcactt caacagttat gtccatgtcc ttgagtttgg gctggacgct ctcgactata
     2881 aggccttccc tggtttgaac atacaccatt gaatctttca gggcaggttc cggttttata
     2941 acggccccta tgactgttcc agagcgctcg acaagtttgt caccaatgtg tattccttca
     3001 attatctcgg gattgacatt ttcagccctc aagacaacaa cgtaggaatc aaattttgga
     3061 tggacaaaat acttcgtgat aatctttgtt ccaccccaat agagcccata cagtagaacg
     3121 atcaggacca gaaggtcaat gatgttcagc tttccaaaaa gtctgccttt ttcgtcgagt
     3181 attaccatcg tcccaccttt ttagggattt caaatctcct ttatggcaag agcaccatct
     3241 gaaacttcaa ccctagcggt tttgccatct ttgagctttc ctgtcaagat caaatcggca
     3301 agcctatttt ccagttcctg ctgtattgtt cttttgagcg gcctggcacc atactcaacc
     3361 gaatagccaa tctcgcccaa aagctcctgc gcttcctttg taacatcaag ttttattccc
     3421 tttttgtcaa gcatcttctg caccctggaa atctggattg aaacaatctt tccaagttct
     3481 tccctggtca gatgcttgaa tactattatt ccatccagtc tgtttacaaa ttccggcctg
     3541 aagttctttc gcacggcctc catgtaaact tccttcatct tgccataggg catgtccggc
     3601 tcaaccatct ggctgccaag attgcttgtc attatgatga tgcagttcct gaaatctaca
     3661 gtcctaccct ggccatccgt gagtcttcca tcatcaagga gctgcaaaaa aacattgaaa
     3721 atgtcctggt gggccttttc gacctcatca aagagcagca cctgatatgg cctatgcctt
     3781 acggcctctg taagctggcc accttgctca aatccgacat atccaggtgg tgcaccgatc
     3841 aatcttgcaa cagtgtgctt ctccatgtac tccgacatgt caaacctcgt cagagcctga
     3901 tcggaatcaa aaagcaattc tgccagactc ttggcaagct cagtttttcc aacaccggtt
     3961 ggaccaagaa aaaggaatga tcctattggc ctgttcggat cagaaagccc ggcccttgag
     4021 cgcctcactg cctgcgcaac agcagttaca gcttcatcct ggcctatcac cctctcatgg
     4081 agtctttcct cgagtttgat gagcttttgc tgttcggatt cggtcagcga tgaaactgga
     4141 atacctgtcc accttgatat tgtctgggct atctcttcag ggccaacctc ttcggaaacc
     4201 ttgacttcac ggagccactt ttcacgctct gatatatatt ctgcattgca tttttcatat
     4261 tccgtcttga gcttcatggc cctgttggtg tcaccggact tggctgctgc ttcgccttcc
     4321 ctctgcagtc tttccgtttc cgacttcagg gatgccagtt ttggcggcat ggactccatg
     4381 gtgaccctta tcctggcggc agtttcatca atcaggtcta tcgccttgtc aggcaaaaac
     4441 ctgttggtta tgtatcttga cgagagctct gctgcagtgg caagcgcctc gtcagaaatc
     4501 tttacaccgt ggtgtgcctc gtacctgtcg cgaagccccc tcaaaatctc gacggtttgt
     4561 gcaactgttg gctccggaac attgacaggc tggaaccttc tctcaagcgc agaatctttt
     4621 tcaatatact ttgtgtattc atcggttgtt gttgccccaa tgcaatggag ctcgcccctt
     4681 gcaagtgcag gcttgagcat gtttgaggcg tccatcgccc cctcagcagc accagcaccc
     4741 atgacaaggt ggagctcatc aatgaagagg attatttttc cttcggcctt tttcacctca
     4801 tcaatgaccg atttgagcct ctcttcgaat tctcccctga attttgttcc cgcaacaaga
     4861 gcacccatgt caagcgacag tatctttttg cccctcatcg aatctggaac attgctatca
     4921 acgatcctct gggcaatccc ttcgacaatt gcagttttgc caacccctgc ctcacctatg
     4981 aggactggat tgttctttgt tcttctggaa aggacctgga ggcttctctc tatttcaagc
     5041 tccctgccaa tcacagggtc aagcttgccc ttttttgcaa gatcggtaag gtctctcgta
     5101 aatttatcca gggcggtttt tgcctgcccg gagcctcccc cttcgccacc ccttattttt
     5161 gctagaccct gaaggatggt ctctctgtcg aggcccctct gggtcattat tctccctgtt
     5221 tctctggtgt cctgggaaag cgctataaac agatgctctg gccccacaaa ctcgtcccca
     5281 agagagtttg cttcttcctc cgccctttca agcgtggact ttgcccttgg cgtgatatag
     5341 atttgcgacc ttgtggtggc ctgggttggg tgcggatggc tggccagcgt cctttcaact
     5401 tcctgcagga tgtccctcca gccttttcca aggtttgaaa gtatttccct gacaacactg
     5461 tcttccttct caagcagggc gacaaagagg tgttcgttat caagctctat gtcatggtaa
     5521 cgttcaagca cctgctgtgc atcagacata acatctttta gtgcatcagt atagcgctca
     5581 aatttcatcc ttcacctctc gaacaatggt aatttggacg cttgtttcag tccttatgat
     5641 tatccttttc cgtttcagca tcatgagcgc gtaagaacag gaaaaacagc cgctgagatg
     5701 gtttgccatc gcaagtgaaa gtaggggatc aagttccccc ctctcataca gtgccaaaag
     5761 tttcaaaaca tcattgcatc tcattttgga ctgccaattt taggcctacc ggagttttca
     5821 gtcaacgcca taggcataat tccttctttt ctttggtttt ctgaaacaga ggaaaaactc
     5881 ggccagacac ccaatcccaa tcgaaagaac aattccctgg gccatgttca tcatgaaaaa
     5941 gaacggccag atgtagccgc catggaagaa aaatgcatca atgatggccc acagcaatgt
     6001 tagaggaatc cccactattc caacgagacc tacaaatctg agcccaaaag cgccgaaaat
     6061 cccaggcaga aggcccaaaa caacaaggcc ggaaaggata tagcttgaga atggcgggga
     6121 ggagatccca acaaccagcc agccaacaag gccaaaggcg gcgccaccga gaacggcgag
     6181 cgccagccag gccagagtca gtttattcaa tggacctttc tttaacttcg gcaagtatcc
     6241 tcgcctcgaa ttcttcagtt gtcatttgcc ccagatctcc ttttccatgc ctcctgaccg
     6301 acaccttccc ggcctcggcc tctttggcgc caaccacaac aacgtaaggc acctttttaa
     6361 gttctgcatt tcttattttg gcattcatcc tgtccgagcc ttcatcaatt ttaactctta
     6421 tgtcagcatc aaacagtctg tcttttaaag atttgcaata gttgagaaca ccgtcagtta
     6481 ttggtacaat gacgacctgg gttggtgcca gccacaccgg aaacgcccca gcatagtgtt
     6541 caatgaggca acccatgaag cgctccatgg aaccaagcag ggccctatgg attgtatatg
     6601 gcctgtgctc tttgccatct tctccggtat aggtaagatt gaatctttca ggattgttga
     6661 aatcaaactg gcaagtcgtg cactgccatg gtctcccaag ggcatcgact atcttgatat
     6721 cgatttttgg cccgtagaat gcaccaccac cctcatcaac atcaaacgat aaacccattt
     6781 catttagcgc ttctttcagg gtgctttcgg caatgtccca tttttctatt tcgccaacat
     6841 acccctcttt cggccttgtg gagaggaata cttcatattt tttgaagcca aatgtttcga
     6901 gcaaatggat ggcgaacttg accacaccaa caacttcctg cttgagctgt tccggagtgc
     6961 agaaaatatg ggcatcatcc tgcgtaaaac ctctgaccct caaaaggccg tgaagggtcc
     7021 ctgaacgttc aaaacgatag acagtaccca attctgcagt cctgtatggc aggtccctgt
     7081 atgatctgac ctttgacgca taattgacca tgtggaaagg gcaattcatt ggccttgcgt
     7141 agtactctac agtttcaagc tccattgcag gatacatgtt gtctgcataa aaacccaaat
     7201 ggcccgatgt tctccagagg tcggcttttc caacgtgagg gctataaacg atctcgtagc
     7261 ccctcttcct gtgctccgtc ctccagaaat tttcgattat ttccctgact acagcgccat
     7321 tcggctgcca gattactagg ccaccaccga catgctcgtc gatgatgaac agctcaagct
     7381 gtttgccaag tatcctgtgg tctctttttg ctatttcttc gaggcgcttg atgtgctctt
     7441 ccaggccatc ttttgtcgaa taagaggtgc catagattct tgtgagcatc tgcctctttt
     7501 cgtcacccct ccagtaggca ccagcgatgc tcatgagctt gaagtgcttg atgtagccgg
     7561 atgatggaag atgtgggccc ctgcatagat cgataaaatc gccaagctcg tatatggaaa
     7621 cggttggatc aggtatgtcc tggatcagct ccaccttgta ggtgtcacct cttgccccaa
     7681 aaaatgcaag tgcatcctct tttgtcattg ttttgcgcac aaatttgatg ttctcgtccg
     7741 agatcttctt catctcggcc tctatttttg gcaggtcctc ctcaaccaat ggctttggga
     7801 gatcaaggtc gtaataaaat ccgtccttga ttgacgggcc aatggcaaat ttggtcccag
     7861 ggaaaagcct gttgactgct gcagccatga ggtgagccga agaatggagc aggatgtctc
     7921 ttccttcagg tgaatcgatt gctatcacct caaaattcgt gttttcctca agcatggtgg
     7981 aaaggtcagc cagcttgtcg ccaatttttg cagcaacaag attttttgtc ggtatttcca
     8041 gcgctttcag cgcctggaat atatttgagc ccttttctat ctgggcagat tttcctgaac
     8101 agttaacact aatcattgtt cctcctagaa tatttttaca ttgttgattt caagctcata
     8161 ctcgcacccg agaaactggg cagacttgcg catcgcctgc attcttgagg caaaaatctc
     8221 aagatagtcg gcagtgcagg cgatctgggg gtcaaggcga agtgagagca aaactttgcc
     8281 ttttgaggca gaaacattgc tctcgagcac tgcatagttg accctatcat gtatatcggt
     8341 cttgatgtcg gcggttggcc tgactcttga cctgtttaca tcgctcttgt ctgcaatgat
     8401 ggtcacggcc gttgtgacag tggaaatgac gggtttctcg tcatcgtcat ggcacgaaat
     8461 ggctgtgata acccttgcaa gctcctcggg caaaagacca tgctgcacca gaaaaggaga
     8521 aaccagtgtt gcacccagtg ggccatggtt ttctcttccg gtcaggtttc cgatgtcatg
     8581 aagaaaagag gcagcgcagg cgacatcggt ctcccactgg gacaagccca gtccggagca
     8641 tatcttcctg gccgcctcac aagtgacggt ggcgtgcctg ataccatgct ccgtatagcc
     8701 aagggcagct atctgcctgt ccgaggcctt caggagctcg acaatgtaag gatcgttttc
     8761 aatcctgtcc agttgaatca tcttctctcc aatttagcca ctttgacgca tactggtcaa
     8821 aggttccatc aattatagcg tttcttgctc catccacaac acgcttaagg aaatggaggt
     8881 tgtgtattga tgcgagagtg gcggcaagca tttcttttgc cttaaaaagg tgcctcagat
     8941 aggccctggt atagttcctg caagtgtaac aatcacagcc ctcttctatt ggccggaaat
     9001 cctttgcgaa tcttgatttt gaaatggaga gtgttccttc ttttgtcatg actgcgctgt
     9061 tccttccaag cctggttggc aggacacaat caaacatgtc aattccggag gttattccct
     9121 gaatgattcc agcagggtca ccaacaccca tgaggtacct cggtctgtcc ttgggcatcc
     9181 tggggaccac aacatccaga atctcccctg caagctcaag cggctcacca acagacagcg
     9241 agccaactgc aaaaccgtca aagtccatgg aagttatggc atcacatgac caagccctgt
     9301 cagtaagttc aaaaccaccc tgggttattc caaaaacaag ctggtcaggt tttgatcttg
     9361 cctcaagcga acgcttcgcc cactcatggg tgatttccgt gtaatgtcgg gctttttgct
     9421 gtgcaatcgg gtatggtgtg cagatgtcca gcaccattgc tatgtctgaa cctagctttt
     9481 cctggattcc tattgcactc tccggggtga gaaaatgggc tgaaccatca agataagatt
     9541 tgaaatggac accttccctg gttatctccc tcagactcgc ctggctcatg acctgaaaac
     9601 cacctgaatc ggtgagaatc gatccatccc agttcatgaa cttgtgaaga ccaccagctt
     9661 catgaacaag gttttcgcct ggctggagca tgagatggta ggtattgcaa agaatcaagc
     9721 caaaaccaat ctccttgact tgctctggtg tcaatgcctt tactgtggct tgcgttccaa
     9781 ccggcatgaa aactggcgtc tttatttcac catgtgcagt tttcaaaata ccggcccttg
     9841 ccctgccgtt tgacgcctca agtttgtagc aactcatttg tcttcgagac gcctttctat
     9901 taagtcacag acttcctgta gggtaaggtt tgtccttgct acactggtat caaacgctgc
     9961 ttttgcaact gacttggcga ctgcctgggc aactcctttt gcgaagggct ctattattat
    10021 tctgtcttcc ctcagctcgt tttctggaag caggtttgcg attgtcatcg aagccgcaac
    10081 cttcattgcc tcgttgatga cacgtgctct ggaatcaaga gcacccctca ttataccagg
    10141 gaaagcaagc agattattta cctgattacc caagtcagac cttcctgtgc aaattatttt
    10201 tgctccggct gattttgcct cttccggtga aatgtcggga tcagggtttg agagggcaaa
    10261 aacgatcgga tctttggcca tttttttaat gtcttcgcca gcgacaaggc cagggcaggc
    10321 aagcccgata aatatatcag cgccaacaag cccctcgtgg agtgttccct taaaattctc
    10381 tttgttcgtt atctcggcaa gtttgtattt ataaggcatc atgtagtcgg tccttcccct
    10441 gcaaatgaca ccctcgcgat caaatagtat gacatcacca attcctgcgg caacaagcaa
    10501 tttggcaaca gctgaaccag cagcacccgc acctgaaatg atgactctct ggttttctat
    10561 ttttctgcca agcagcttga gtgcgttgta tatgccggca agcgcaacaa cagccgtgcc
    10621 gtgctggtca tcatggaaaa ccggaatatc caattcctta attagtatct cttctatctc
    10681 gaaacagtga ggtatctgta tgtcctcaag gttaattccg ccaaatatgg gttccattgc
    10741 ttttacggcc gcaacaaatt cctcatttgt tcttgctttc aatggtagag gaaatgcgtc
    10801 aattgcacca tattcaacaa aaagggcgca tttcccttcc atgactggta tcgatgcctc
    10861 agggcctatg tttcccaatc ccaagacacg actaccatcg gtcactacag ctatggtatt
    10921 gccctttatt gtatagtcat aaacagtctc agggtttcta tatatctgcc tgcatggttc
    10981 ggcaacccct ggggtgtacc acaccgacag tgcgtgcttt gacctgagat cgtcttttac
    11041 atcaaccctt attttgcctt tgtgcaaatg gtgaagcttt aaggattctt cgtcaaatat
    11101 actcatgttt catctccttg gtttcctgac gggtgaatat gatagggaaa tgccctaaaa
    11161 ttgaaagtca aaaatcattt ttttacttga cttcattttt tgcgggactt agaatttcca
    11221 atcaagtgat ggcatgcgct gtccacctga cggacgatcc tcaggtcatg acgcaagcgg
    11281 tacgcagata gttttttaag tcgcaccagg cgacaggagg tatgcaatcg aaaagaagtt
    11341 gcgcacaaac aaggagattc gtgcacgcga agtgcgaatg attgacgatg aaggcaaaca
    11401 ggttggaatc gtcgacataa gaaaagcact cgaaatggcg gtcttgaaag ggcttgactt
    11461 ggtagaaata tcaccaaatt ccgatccacc agtatgcaag ttgctagatt acggaaaata
    11521 caagtacgag cttgaaaaaa aagttaaaga aaaccgccgc aaacagtctt ctgtggaaat
    11581 aaaagaaatg cagttttcgc tatcagtcga cgagaacgat taccaaacca aagttggcca
    11641 cgtaagaagg tttctgctca acggtcaccg tacaaaggtt gtcataagat tcaggggccg
    11701 cgagatgatg cacaaggacc agggacgtgc cctccttgac cgtatcgcaa atgaagtcgc
    11761 aagcctcggg acaatcgagc aaatgccaaa gatggatgga agacacatgc acatgatgat
    11821 tgccccgaca aaacaaaaag agaaggaaat aaaataagag aggtgatgta tgccaaaact
    11881 taagattaga tcaaaaggct cagcaaaaaa acgtttccgc gtcaccggaa ccggcaagct
    11941 ccgcagtcaa agccccggct ttgcccactt gcttgagaac aaaaacaggg gagagacgaa
    12001 gcgcaaaatg cgccttaaag atgtcgacaa gacaatgatt cgcctaatga agtgcctcct
    12061 cccggttgga gtgaagggac gcaagatcag aaacgagaaa aaggaggact aagagatgcg
    12121 cgtaaaaggt ggaattgttg cgagacgcag gaggaaaaaa gtattaaata gggcatcagg
    12181 tttctttggc gctgcccata ggcgcttcaa aatagcaaaa gagcaggttc ttcacgctga
    12241 aagatacgaa tttattcata gaaggcaaag aaaacgtgat ttcagaaggc tctggatcac
    12301 aagaatcaat gctgcagcaa gaatgaatgg aatcacctac agccgtctcg ttgccggact
    12361 caacggtgct ggctttgaga tcgaccgcaa gatacttgca aagcttgcaa tcgaagatgc
    12421 aaacgccttc accaagctcg tggagcttgc aaaggccaac ctcaagaact agatgccgag
    12481 actttttggc accgatggtg taaggggaat agcgggagac gagctcaacg aggagcttgc
    12541 ctcccgtctt gcttttgcgg catcccagca atttttcaca aaggacggaa aagtcctggt
    12601 cgggaaggat acaaggcttt caggcgatat gattgaatcc gcactggcaa gcggttttgt
    12661 ttccggcggg tgtgatgtta tttcggtagg aataatacca acaccgggcc ttgcaagatt
    12721 gtgcaaggaa acaggtcatt ttggggccgt catttcggcg tcccacaacc ccattgaata
    12781 caatggaatc aagttttttt caccgcaggg cgtaaaactt agggatgagg aagaggacga
    12841 gatagaagcg agaatgtccc aaaaaccaaa cggccacaca aggggtactt tcacaaaaaa
    12901 tattaacctt tgtgaacgct attgccagga cattgcttct ttcttcaatc tcgatatggg
    12961 aggcagaaaa atcgtcctgg attgcgccta tggcgccact tatgccgtgg cacccaaact
    13021 ttttaaaaga ctcggagcag atgttattgc aatcaacgct gaaccggatg gatcaaggat
    13081 aaatgtcgat tgcggctcaa caatgccaca catgatgtgc aaaactgtcc tggaacaaaa
    13141 agcagagatg ggttttgcct ttgatggtga cggcgacagg tgcatcgcct gcgactcgca
    13201 gggcaggatt gtcgacggtg acaagatgat gggcatcctg gccaaatata tggtaaaaaa
    13261 gaaccgtttg ccaggaatgg ccatcgttac aactgtcatg actaatcttg gtcttgaaga
    13321 acacctttcc agttgtggca taaagatgga gagaacaaag gtcggcgaca gatatgttca
    13381 ccagagaatg accgaaaaag gcctcaatct gggcggagaa cagtccggcc acataataat
    13441 cgcggacaga acaacgacag gcgatgggac gctttcggcc atttcccttg ttgaagccct
    13501 ggaggaaaca ggcgcaagcc tttcggacga gctggacctg attcctgaat atccacaaat
    13561 cctcctgaac gtgaaagtca gggaccgcga ggaagcaatg aactctctgc tggttgcaga
    13621 actcacccaa aaagcccagg aaaagcttgg aaagcatgga agaatcctaa ttagatcttc
    13681 tggcaccgag gccctgataa ggataatggt tgaagctgtc gatgaagcgc tctgcaagga
    13741 aatcgctttc gacacgacag agaaaataaa atccagatac ggagtatgaa cctaaacccc
    13801 gaccattgct ggtcggggtt tttaatttta ggcccaaaaa atgacaaaaa tatccctatc
    13861 acaagcgaaa cagacgatca taaagtgcca gttgcttgac aaaccggcag gcaaaggcaa
    13921 agcgggcgcg ctgaaggccc ttgagcacct tggctatgtc cagattgaca ccatttctgt
    13981 catagagcgc gcccaccatc atgttttctg gtcaaggtgc cccgatcaca aaaaagagca
    14041 ccttcatgaa ctgcaggcaa aagatagaaa ggtctttgag tactggggtc atgcggcatc
    14101 ttttcttcca atgtccgatt tcaggtattc gattcccgta atggaagcac acagacaaag
    14161 agacgatgtc aaaacagtgc ttgataccta taaggaatta tcagtgcaag tgctggacag
    14221 gataagacag gaaggtgctc tgtcatcatc agacttcgaa acaccagagg attttgaaag
    14281 gggcccgtgg tggggatgga aaccggcaaa acaagtgctg gaggcgcttt tctggacagg
    14341 tgaactgatg gtgtcacaca ggaaaaactt ccagcgatac tatgaccttc cggagagggt
    14401 tatcccgtct tcgacaaacc tggaaaaacc ggacgagggc aagcttgcaa agttcagggt
    14461 catggcggca ttgagaacgc tcggtttcgc aaatatccag gacatctgct ggatgagaag
    14521 gaacaaaatc gccatgaaaa aagccctgca ggagatgatc gaggagggcg aagcagtcga
    14581 ggttgaaatt gaggggctgg acggcacata ctattgtctg gcgaacaacc tttcggttgg
    14641 cgaggaaaag aacggcctca actgcaatat cctttcgcca tttgacaatg cagtgataag
    14701 acgggaccgg ctggggaaaa tatttgattt tgaatactct tttgaatgct acgtccc
//
LOCUS       DAOSRI010000004        13607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4607_length_13607_cov_4.095632, whole genome shotgun sequence.
ACCESSION   DAOSRI010000004 DAOSRI010000000
VERSION     DAOSRI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_13607_cov_4.095632"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PK717_00250"
     CDS             complement(<1..1530)
                     /locus_tag="PK717_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOR46136.1"
                     /translation="MITIEVNGKKLVAKPGEMLLSVLRRNGIQVPTLCNLEGLSPTGA
                     CRLCVVEVEGQRGLVPSCAFPVSDGMKIKTHSPRAVMARKTIIELLLANHPDDCNYCV
                     RNGTCELQNMAHELGVRERKFVGAKNKYFIDKSSPSIVRDPAKCILCGKCVRTCEEVQ
                     GVSAIDFVNRGSRSIVGTAFGKGLNVSSCINCGQCILVCPTGALTEQSHIPMVLDALK
                     DPNKFVVIQHAPAVSVTLAEEFDMKPGVDINGVLVTALRMLGFDRVFDTSFSADLTIM
                     EEATELVHRITTGGKLPMFTSCSPGWIKFVETFYPDFMDNLSTCKSPQEMMGAIIKSY
                     FSQRENIDPDKIFVVSVMPCTAKKFEAGRPELGTNGIPDVDAVLTTRELAQLIKMFGL
                     DINSLPTDTADTPFGRRSGAGKIFGATGGVMEAAIRTAYYLITGEDLEELKVQPVRGM
                     KGIKEARIKIGDLELGVAVVSGLGNARELLEQLRRGRNDLQFIEVMTCPNGCVGGGGQ
                     PI"
     gene            complement(1544..3496)
                     /locus_tag="PK717_00255"
     CDS             complement(1544..3496)
                     /locus_tag="PK717_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOR46137.1"
                     /translation="MQTATDFALTKENLGAYLTGSLSGDSAKGIEAMLSKLKREDVSK
                     PTIYVGTGTCGLGAGAGKTLEAIKVYLAEKNVDADIIQVGCIGLCAEEPMVDFQLPGR
                     TRISFRKITEDKVPKLMDAMLAGNVPEDLACFQFRSKTLKGWNNVKYMDEHPFFASQT
                     RWVLAGCGIVDPTNIWEYIASDGYKAFAKAIYDKTRQEVCDMVEKSGLRGRGGGGFNT
                     GTKWKLALQQEADQKYMICNADEGDPGAFMDRAVIEGDPHRMIEGLAIAAYAIGASKA
                     YVYLRAEYPLAIKHLQQALKQAKEIGLLGENILDSGFDLEIKIKMGAGAFVCGEETAL
                     IHSIEGKRGMPRPRPPYPSVKGLFGKPTAINNVETLANVPTLVRNGEGWFSSIGTATS
                     KGTKVFALSGNVNNTGLVEVAMGTNLRKIIFEIGGGIPKGRQFKSVQIGGPSGGCMPT
                     PLLDTEIDYESLKSAGAMMGSGGMVVMDDATCMVDVAKYFMDFIQRESCGKCIPCREG
                     TRRMLEILTMITKGRKGEHGADALERFQGITQLERLAEVIKDTSLCGLGNTSPNPILS
                     TLRWFKPEYEAHIYERRCPAGVCPELLVYSIDADKCTGCTLCAKNCPAASIMGAPKSP
                     HYIITDKCISCGTCFTVCRFGAVSKS"
     gene            complement(3480..3965)
                     /gene="nuoE"
                     /locus_tag="PK717_00260"
     CDS             complement(3480..3965)
                     /gene="nuoE"
                     /locus_tag="PK717_00260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOR46138.1"
                     /translation="MTKQDIEPILKKHQNAGRDMLIPILQEVQEQYGYLSQEAVIRIG
                     EFLNLPTSKIYGVATFYNQFRFQEPGRFHVQICRGTACHVKGSAGVLEALKRELKIEP
                     GQTTRDGLFSLEIVACMGVCGLAPAMNVNGEVYADISTEKIKKILSEYRRKAEKDANS
                     N"
     gene            complement(3946..4632)
                     /locus_tag="PK717_00265"
     CDS             complement(3946..4632)
                     /locus_tag="PK717_00265"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOR46139.1"
                     /translation="MKKNGKIEKVSEKTIGRLSLYRRAMDKLAKEGRDFAFSHEIAHM
                     SGATPDQVRRDIMVLGYSGSPNRGYSVAELSKSIGEFLDDLDVQKVVLVGVGNLGRAI
                     LSYFAGKRPKLSIIACFDNDTEKTGRVINGCRCYPMSHLQDVINENSASIAIMAVPFS
                     ASQEVADRLTHSGIKGILNFTPVSIKVPHGTYVEDIDMTMALEKVAFYSRQKPQGQLL
                     ERRTNDKAGH"
     gene            complement(4792..5982)
                     /locus_tag="PK717_00270"
     CDS             complement(4792..5982)
                     /locus_tag="PK717_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOR46140.1"
                     /translation="MQQVASLLLSFIVGLFGVSGPGQTGRNFGAIEGYYGKPLSLERR
                     ISLIRTMGNIGLNFFMIAPKNEPNLAKRWRDEFPEAFKTEMSQLAGICKENGVELCLS
                     ISPGSKFNRGEIALLADRAYQLSFYSPNYFTLLFDDIGVLRSSNLGKLHAEASNAVLS
                     SIRKRNPFARLLVCPTQYFGNRKSDYLCNFLVNLDRSIIVFWTGPDVVPEEISGQSFG
                     DFKKMVGRDVAIWDNYPVNDFNPKCLWLGEYCGRHLSVARDSFGLFLNISERSEISKI
                     SLAHFADFLGASPCGSMLAYVMDDLFPLHKEAANSLLKIFDVRPGRKTFSWSGVTASF
                     KNDNKSLLKDLLAVASLSQVGNKMLEEDLRPWTESAKILSDAISAKKITPNLLDWFPT
                     LKEK"
     gene            complement(5982..6782)
                     /locus_tag="PK717_00275"
     CDS             complement(5982..6782)
                     /locus_tag="PK717_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46141.1"
                     /translation="MDNNRTILGVVGLITFALVFLFNYQVGASPAPWSTTGKPVAWAI
                     WADAVKMLAPFATSLCLLYQGFFGNILDSLKSTVKLLGLGTLLLGIGILLEFLAHLVG
                     IDAPTSSASSVFYICGVFLVIWAFFSFPVRLGYEMSRKQKIFFTVMVVILSLITFFIM
                     FFPFMGHPDKLVAEFGYHDAMIASLYVISVVIFFAAVLRLAILFAKSKIGRPFRFFAA
                     SGFLIVAYEYYVWFPYTPNISVFNMINLFWIVGFLLGMVGGLDLTLQS"
     gene            complement(6866..8452)
                     /locus_tag="PK717_00280"
     CDS             complement(6866..8452)
                     /locus_tag="PK717_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46142.1"
                     /translation="MKKTLLFVTGFVLLAALGLVWYFTRYVPPKIPELAIPGLETVSE
                     VERDYSHVLLSATVKYLGNSYVCSLDLKIGSDSKLILPTKDQVFTAIVSEEDGLELWR
                     DNRKIGKLPIVFISDKNEDGSIYLVGPAGESLRPVFVKSGMVNLATFKYDTIIRGQEG
                     VRVQIYKETKQIDDGKLPMMVRLGDKIGRADIKDGLQVWGIGGTGVFNVYAGYTQLKK
                     GQNEVMPVGRFTGSDAWFDKGKFLGASDVAYALSGEKVNIIGEGQKEVTVPGIDTTLV
                     EGDPVLADGSIVLGSNGKLVSLDKTGAIRELGFAPSGKAMVSGNLIISGKDAFDTKIR
                     KTYKVPDKYNFLADGVFVTIQNGKLLARSFVDGSLKTLWEKPCKIDDGPSISIVSGSL
                     VFLYKGDRCTEFFDVGTGKSGYDQDLLVEKPEIEVVQGEGLCFPSGLRIKNGIVCVNQ
                     KGKMVWGRAVENQKRFGAFGVWVNSKTTAVNTIYDVRNGNEIMYWEWPTIVPKIINAE
                     YLCFVSGNDHTVFVKRTLKK"
     gene            8538..10946
                     /locus_tag="PK717_00285"
     CDS             8538..10946
                     /locus_tag="PK717_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46143.1"
                     /translation="MELTKPGYYHDMERKKGTGFLLLILLVFATTPTPQPTASDSCTT
                     KPYRNILSFPASSWPSFRGDPQNTGCTTASQAPKEIMQEIIWSKPGDSSIDCAVVGEG
                     GKIYVGYFDKKLLCLDGESGKELWTLRTDGGIVCSPTIFMGKLYFSTWQKTFYCVNAE
                     TGEKEWERKAEITTPVICSGDLLFYGTASGKIVCESIASKKTMWTFETGQTIAFACPT
                     IFEGKVCLGDWGGTLHCLDQKTGRETWSFKAGGAIKSAAAFSNNSLYFGCMDGTFYCL
                     NSETGKSNWTFDSGSPVESSPAVGAGRIVFASYYNIHCLNEQTGTPLWSFKPKFRAIS
                     SPIIAGDKVYQATLCQSLHCFDLEKGTEFWHYEDLIIHSSPSCMSGMIYIGNDHGTLY
                     CFGDTKAGGKQEPLFSIEPAEMVFERPGTEKQLKVTNLTGDYLSLNAKTGEAWIKLSK
                     TVFELAPQKTAYIMVSVAWELGTDEEMAGYITIENETWEEIARVIVKKKNTEDFCQWQ
                     MFRGGPERTGHPDGECGPKDPSSMEALWSFQAWQGAVESSPAIWNNSVYFGSYNNNFY
                     CVDIDTGELKWRYECKDGIYSSPAIDDSKVYFGSFDSYFRCLDCKDGKEVFKFKTGDR
                     IHSSPLVSSGKAIFGSNDGKVYCLDPKTGDQKWVFEANTPIQSSPALKNGRIFIGGLN
                     GTMYCLDFETGNIIWEYNAGGSITCVPALAYDRVYFGTWARKFCCLAQEDGKLQWSFD
                     TKEYIESSAACSSGRVYFGANDNYLHCLDGLTGDEIWTFRSQDRKVTSSPAIDSNEKI
                     YVGT"
     gene            complement(11158..12084)
                     /locus_tag="PK717_00290"
     CDS             complement(11158..12084)
                     /locus_tag="PK717_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46144.1"
                     /translation="MKRKIIVTVSLLALLVVGTVYARGLIVLSPSEVQLAAKADSTVI
                     SKVDIINGTTFDQDYTVESSADWLQLDHKEGKVLVRQPSELKLTCSTKDMKPGMHVAQ
                     IYVNEVTDDPSGGASAMLNVSLKVYESDPRLEFVPRSAYIGLGQVMTIMVLNPSEIDL
                     NVDIMPKDPWVLVYPPTLEIPSRSMRVVVIKTSSTAVSAGSFPTSLDIKGGGIEVNYP
                     VVVNVESGLSFNPSEIITNEGQIVVTNNLKKRVVIEARQATGLEIDLKPFALQPKAKK
                     IYNFKLLENHPDAITFRFSGALTDTQNIPIKK"
     gene            complement(12182..13294)
                     /locus_tag="PK717_00295"
     CDS             complement(12182..13294)
                     /locus_tag="PK717_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46145.1"
                     /translation="MQRVLSFFLALSVLLSPLAVTAAFPPERAIWSQRGYDPLKTGAI
                     PWESGPNFVMSGEAIKAKETIQGVSTYGDLVICSGTNGSVFTLAKWGAAVWATRLDSK
                     PIGVPAFSRGHLFVLKQNGKLAKLKENNGSIVWDIKMVEEGQTDITVVGDRLGVFAGK
                     KSVVCVDLETGIRVWEKAISGNVQPLSCQGTFVLVNSENELSCFLLGNGNKVWSRKLE
                     TGFAGVPVIFGDKVYVGTLDEKIMCLSLWAGETVWQAERGYSGAAPTVADGKVWVPGN
                     GIIWCYGADDGKLVWKSVLDKKSDFIFWNQLAKSGNLLIASGNSPDLYILDATNGQTK
                     KKLKVSSGVGVDLAFGFDYMVAIDAKKQIFIFGFSK"
     gene            complement(13327..>13607)
                     /locus_tag="PK717_00300"
     CDS             complement(13327..>13607)
                     /locus_tag="PK717_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR46146.1"
                     /translation="EGQSLKIKLEAKCEGETLLASFADNGIGIPKSKQNFIFKPFYQV
                     NPEGDLKNGMGMGLSIVKMHVESHGGSVSFSSSEGAGATFFIRLPLLS"
BASE COUNT         3731 a         3366 c         2909 g         3601 t
ORIGIN      
        1 aatcggttgt ccaccaccgc caacacaacc attggggcaa gtcatgactt caataaactg
       61 gagatcattc cttccccttc tcagttgctc gaggagttcc ctggcattgc caaggcctga
      121 aacaacagcc acaccaagct ctaggtcacc tatctttatt ctggcttcct ttatcccctt
      181 catacccctg accggttgca ccttgagttc ttcaagatcc tcgccagtaa tgaggtaata
      241 agcggtcctg atggctgctt ccatgactcc gccagtggca ccaaatatct ttccggcccc
      301 tgatcttctt ccaaatggtg tgtcggcagt atctgtcggg agtgagttaa tgtccaagcc
      361 aaacatcttg atgagttggg caagttctct agttgtcagt actgcatcga catctgggat
      421 tccattagta ccaagctctg gtcttccagc ctcaaacttc ttggcggtac acggcattac
      481 tgatacgaca aatatcttgt ccgggtcaat attttctctc tgcgagaagt acgatttgat
      541 gattgcaccc atcatttctt gagggctctt gcacgtagac aaattgtcca tgaaatccgg
      601 gtagaacgtt tcgacaaatt ttatccagcc tggggagcac gaggtaaaca tcgggagttt
      661 tccgcctgtt gtgatcctgt ggacaagctc ggtcgcctct tccatgattg taaggtcagc
      721 ggagaatgaa gtatcaaaaa ctctgtcgaa acctagcatc ctcagtgcag tgaccagaac
      781 tccattgatg tcaacaccag gcttcatgtc gaactcttcg gccagcgtta cggaaacagc
      841 cggagcgtgt tgtattacaa caaatttgtt cgggtccttg agtgcatcaa gcaccatcgg
      901 tatgtggctt tgttcagtga gcgcaccggt tgggcaaaca aggatgcact gtccacaatt
      961 gatgcaagac gacacgttga ggccctttcc gaatgcagtg ccaactattg acctgcttcc
     1021 cctgttcaca aaatcgattg cagagacacc ctgaacttct tcgcatgttc tcacgcattt
     1081 gccacagagt atacattttg caggatctct gacgattgag gggctggatt tgtcgataaa
     1141 atatttgttt ttggcaccaa caaattttct ctccctgaca ccaagttcat gtgccatgtt
     1201 ttggagctca caggtgccat tcctgacgca gtaattgcag tcgtccgggt gattggcaag
     1261 caagagttcg atgatggttt ttctggccat gactgccctt gggctgtggg tctttatttt
     1321 cattccatca gaaacaggga aagcacagct gggtacaaga ccacgctggc cttcgacctc
     1381 aacgacacaa agcctgcaag caccagttgg cgaaagcccc tcaagattgc agagtgtcgg
     1441 tacctgtatt ccatttctgc gaaggaccga aagaagcatt tctccaggct tcgcaacgag
     1501 tttctttccg tttacttcaa tggttatcat cttccatccc ctttcacgac ttggaaacgg
     1561 cgccaaacct gcagaccgtg aagcaggtgc cgcaagatat gcacttgtcg gttatgatgt
     1621 agtgcgggct ctttggtgca cccattatgc tggcggccgg gcaattcttg gcgcacagcg
     1681 tacaaccagt gcacttgtca gcatcgattg aataaaccag gagttctggg caaacgcctg
     1741 ctgggcatct tctttcgtat atatgggcct catactccgg tttaaaccac ctgagtgttg
     1801 aaagaattgg atttggcgaa gtattgccaa ggccacaaag actggtatct ttgatgacct
     1861 ctgcaagcct ttcgagctgg gtgattccct ggaacctttc gagtgcatct gcaccatgct
     1921 cgcctttcct accttttgtt atcatggtaa ggatctcgag catccttctt gtgccttccc
     1981 tgcatggaat gcactttccg cagctttccc tctgaatgaa gtccatgaag tatttggcca
     2041 cgtcaaccat gcaagttgca tcgtccataa cgaccattcc gcctgagccc atcatggctc
     2101 ctgcggactt gagtgactca tagtcaatct ctgtatccag aagtggggtt ggcatacatc
     2161 caccagacgg cccaccaatc tggacagact tgaattgcct gccctttggt attccaccac
     2221 cgatctcaaa aatgatcttg cgaaggttag tccccatcgc cacttcgacc agtccggtgt
     2281 tattgacatt ccctgaaaga gcaaaaacct ttgttccctt tgaggttgca gttccaatcg
     2341 agctgaacca cccctcaccg ttcctgacaa gtgtcggaac attggcaagc gtttcaacgt
     2401 tattgatggc tgttggcttg ccaaaaagtc ccttaaccga aggatacggt ggccttggac
     2461 gaggcatgcc cctcttgccc tctatggaat ggatgagcgc ggtctcttct ccacaaacaa
     2521 aggcaccggc gcccattttt atctttattt caaggtcaaa ccctgaatcc aggatgttct
     2581 caccaagcaa accaatctct tttgcctgct tcaatgcctg ctggaggtgc ttgattgcaa
     2641 gtgggtactc tgccctcagg tagacatagg ctttggaagc gccgatagcg tatgccgcta
     2701 ttgcaagccc ctctatcatt ctatgcggat cgccctcgat gacggccctg tccatgaacg
     2761 cgcccggatc accctcgtca gcattgcaga tcatgtattt ttgatcagcc tcttgctgga
     2821 gcgccagttt ccacttcgtt ccagtattga agccaccacc acccctgcct ctcaggcccg
     2881 acttttcaac catgtcgcaa acttcctgcc tggtcttgtc atagatagcc tttgcaaagg
     2941 ctttgtaacc atcagatgcg atgtattccc aaatattggt tgggtcaaca atgccacatc
     3001 ctgcaagcac ccaacgggtc tgggaggcaa aaaatgggtg ttcatccata tatttcacat
     3061 tattccagcc cttgagtgtt ttgcttctga actggaagca agcaaggtct tccggaacat
     3121 taccggccag cattgcatcc atcagctttg gtaccttgtc ttcagttatt tttctaaaag
     3181 aaatccttgt tctgcctggt agctgaaaat caaccattgg ctcttcagca caaagtccta
     3241 tgcaaccgac ctggataata tctgcatcaa catttttctc ggcaagatat accttgattg
     3301 cctcaagcgt tttgccagct ccagctccaa gaccgcatgt tccggtgcca acatagatgg
     3361 ttggttttga aacatcctct cttttgagct ttgaaagcat tgcttcaata ccctttgcgc
     3421 tatcgccgga aagcgaacca gtaagataag cgccgaggtt ttcttttgtc agtgcaaaat
     3481 cagttgctgt ttgcatcttt ttccgccttt ctacgatact cagaaagaat cttctttatt
     3541 ttttcagttg aaatgtctgc gtagacctcg ccattaacat tcattgccgg ggcaagacca
     3601 cagacaccca tgcatgcaac gatttcaagg ctgaaaagac cgtctctggt cgtctgtcct
     3661 ggctcgatct tgagttcacg tttaagagcc tcaagtacgc ctgcagagcc tttaacatga
     3721 caggcggtcc ccctgcatat ctgcacgtgg aatcttcctg gctcctgaaa cctgaactgg
     3781 ttatagaacg ttgcaacacc ataaatcttg ctggtcggca aatttaggaa ttcaccgatc
     3841 cttataactg cctcctggct tagatagcca tattgttcct gcacctcctg tagaatcggg
     3901 atcaacatgt cccgcccagc attctggtgt tttttcaaaa ttggctcaat gtcctgcttt
     3961 gtcattggtt ctcctttcca aaagctgtcc ttgtggtttt tgtcttgaat aaaacgctac
     4021 tttttcaaga gccattgtca tatcaatgtc ctcaacgtag gttccgtgag ggaccttgat
     4081 agaaaccggt gtgaaattaa gaattccctt gattccagag tgtgtaagcc tgtctgcaac
     4141 ctcctgcgag gccgagaacg ggaccgccat tatcgctatt gatgcgctgt tctcatttat
     4201 gacatcctgg agatgggaca tggggtagca acggcaacca ttgattaccc tgccagtctt
     4261 ttcggtgtca ttgtcgaaac aggcaataat ggagagtttt ggcctcttgc cagcaaaata
     4321 cgacagtatg gcccttccaa ggtttccaac cccgacaaga accaccttct ggacatcaag
     4381 gtcgtcaaga aattccccaa tgctcttcga gagctcggca acagagtagc ccctgttggg
     4441 ggagccgcta tacccgagca ccataatgtc tctcctgacc tgatcaggag tggcaccgga
     4501 catgtgggca atttcgtggg aaaaggcaaa atctcttccc tctttggcaa gtttgtccat
     4561 cgctcttcga taaagcgaaa gtctgcctat cgtcttttct gaaaccttct ctatttttcc
     4621 gttctttttc atattcgtga catctttcac attgtgtaat cgtgcatgag ttaccttatg
     4681 caaagaggaa acaaagtcaa gttagcttgg caaagaaatt ggtaaaatca tataaccttc
     4741 ttccgtcata tgcctgaaga agcccgtcca aatgagaaca ggatgaggaa gttacttttc
     4801 tttgagggtt ggaaaccagt ccaggagatt tggtgtgatt tttttggcag agattgcatc
     4861 cgagaggatc tttgcgcttt ccgtccaggg acggaggtcc tcttcaagca tcttattgcc
     4921 aacctgcgag aggctggcca ccgctaaaag gtccttcaac aaagatttat tgtcgttttt
     4981 aaaggacgcc gtaacacctg accatgaaaa cgtttttctc cctggtctta catcaaaaat
     5041 cttcaacagg gagtttgctg cttccttgtg cagaggaaac aggtcatcca ttacatatgc
     5101 aagcattgac ccacatgggg acgcaccaag aaaatcggca aaatgggcaa gcgaaatctt
     5161 ggagatttcg gatctttcac tgatatttaa aaacaagcca aagctatccc tggcaacact
     5221 gagatggcgc ccacagtatt cccccagcca aaggcatttc ggattgaaat catttaccgg
     5281 ataattatcc cagattgcta catccctacc taccattttt ttgaaatcac caaacgactg
     5341 gccactaatt tcctcaggaa caacatctgg cccagtccag aaaacaatga ttgacctgtc
     5401 caaattgacg agaaagttgc acaaataatc tgattttcta tttccaaaat attgagtggg
     5461 gcacacaagc agtctggcaa aaggattcct ttttcgtatc gaagacaaaa cagcatttga
     5521 cgcctcagca tggagttttc caaggttact agaacgcaaa actcctatgt catcaaaaag
     5581 caatgtaaaa tagtttgggc tgtaaaaaga cagctggtat gccctatctg caagaagggc
     5641 aatctctccc ctgttaaact tgcttcctgg cgatattgac aggcaaagct caacaccatt
     5701 ttctttgcaa atcccagcca attgggacat ctcggtttta aatgcttcag gaaactcatc
     5761 tctccacctt ttagcaaggt ttggctcatt ttttggggca atcataaaaa aattcaggcc
     5821 gatgtttccc atggttcgaa tcagcgaaat ccgcctttcg agagaaagcg gtttgccata
     5881 atagccttca attgcgccaa agtttcttcc tgtttggcca ggccctgaaa caccaaagag
     5941 gcccactata aaactaagta gcaaggaagc tacctgttgc atcagctttg cagtgtcagg
     6001 tcaaggcctc ccaccatgcc aagcaggaag cccactatcc agaacaggtt gatcatgtta
     6061 aaaacactga tatttggagt ataggggaac cagacataat actcataagc aacgatcaga
     6121 aaaccgctcg cagcaaaaaa tctgaatggc cttcctatct tgctctttgc aaaaagaatt
     6181 gcaagtctca agactgcagc aaagaaaata acgaccgaga taacatacag cgatgcaatc
     6241 attgcatcat ggtagccaaa ctcggcaaca agtttgtcag gatgccccat aaatgggaaa
     6301 aacattataa aaaaagttat aagggacagg atgacaacca tgacagtaaa aaatattttt
     6361 tgctttctac tcatctcgta acccaatctt accgggaaag agaaaaacgc ccagataaca
     6421 aggaaaacac cacaaatata gaacaccgat gatgctgacg aagttggagc gtcaattcca
     6481 acaaggtgag caagaaactc caataaaatg ccaatcccaa gaagcaatgt cccaagacca
     6541 aggagtttga cagttgattt cagactgtcg agaatattgc caaaaaaccc ctgatataaa
     6601 agacacaaag acgtggcaaa cggtgccagc atctttactg catcggccca gatagcccat
     6661 gccactggct tcccggtggt actccacggt gcaggtgaag caccaacctg atagttaaac
     6721 aaaaaaacga gggcgaatgt tatgagtcct acaacaccaa gaattgttct attgttgtcc
     6781 atgttcacaa ataaatacta tcacttattt tttcaaacaa aagcctttgc caaaaaggta
     6841 acaaaacaac cctccaagca actggctact ttttcagcgt tctttttaca aaaaccgtgt
     6901 ggtcattgcc tgaaacaaaa cagagatatt ccgcatttat tatttttggg acaatcgttg
     6961 gccattccca gtacatgatc tcgttaccat ttcgcacgtc atagatggtg tttaccgcgg
     7021 tcgtcttgga gtttacccag acaccgaagg ccccaaaacg cttctggttt tccacggccc
     7081 ttccccagac catcttcccc ttctggttta cacagactat cccatttttg attctcaaac
     7141 cagacgggaa gcataggcct tcaccctgaa caacctcgat ctctggtttt tcaacaagca
     7201 agtcttggtc atatccagac ttgccggtcc ccacatcaaa aaactcggtg cacctgtcgc
     7261 ccttgtacaa aaacaccaga ctgccagaaa caatagaaat actcggccca tcatcaatct
     7321 tgcatggttt ttcccaaagg gtctttagtg aaccatcgac aaatgatctg gccagcagtt
     7381 ttccgttctg gatggtgaca aaaacaccat cagccaagaa attatacttg tctggtactt
     7441 tgtatgtttt tctgattttt gtatcaaaag catctttacc agaaataatg aggttacctg
     7501 ataccatagc ctttccagat ggcgcaaaac cgagctctct tatcgcaccg gttttgtcca
     7561 gggaaacgag tttgccattt gaacccagaa caatgctccc gtctgccaga acaggatcac
     7621 cctcgacaag ggtggtatca atgcctggca cggtgacctc tttttgccct tcgccaatta
     7681 tgttcacctt ttcgccagaa agcgcatagg caacatcaga tgcaccgaga aattttcctt
     7741 tgtcaaacca cgcatcggag ccagtgaatc tgcccaccgg catcacttca ttctgaccct
     7801 ttttcagctg ggtgtatccg gcataaacgt tgaaaactcc ggtacctcct atcccccata
     7861 cctgcaatcc gtccttgatg tcggcccttc ctattttatc accgagcctg accatcatgg
     7921 ggagtttgcc atcatcaatc tgttttgttt ccttgtaaat ttggaccctt acaccttcct
     7981 ggccccttat gatcgtgtca tacttgaatg tggcaaggtt gaccattccc gatttcacaa
     8041 aaaccggtct cagactctct cctgctggcc cgacaagata tatcgagcca tcctcatttt
     8101 tgtctgagat aaaaacgatt ggtaatttgc cgatctttct gttgtccctc cacagctcaa
     8161 gcccatcctc ttcagagaca atcgccgtaa agacctgatc cttggttggc aatatgagct
     8221 tgctgtcact gcctatcttg aggtcaagcg aacagacata ggaattccca agatatttta
     8281 ctgttgcaga aagcaaaaca tggctgtaat ccctttcaac ctcgctgaca gtttccagac
     8341 ctgggatggc aagctctgga attttcggag ggacatatct tgtaaagtac cacacaaggc
     8401 caagggcagc cagaagaaca aaacccgtaa caaagagcaa ggtctttttc atggccacat
     8461 tattgcagac aaaaagttgc ctttcaagca ctagacctag accagaaaac ggcaaaagga
     8521 cctaacagaa aattggtgtg gaattaacca aaccaggtta ttatcatgac atggagcgca
     8581 aaaagggaac cggatttctt ttactgattt tgctggtttt tgccacaaca cctactccac
     8641 agccaacagc tagcgacagt tgcaccacaa aaccatacag aaacattttg tccttcccgg
     8701 ccagttcatg gccatctttc aggggtgatc cccaaaatac cggctgtaca acggcatcac
     8761 aggcaccaaa agaaattatg caggaaataa tttggagcaa acctggagat tcctcaatag
     8821 actgcgctgt tgttggtgaa ggcggaaaaa tctatgttgg ctatttcgac aaaaagctcc
     8881 tctgccttga tggcgaatcc ggaaaagagc tatggactct aaggacagat ggtggcatag
     8941 tctgctcccc cacaatcttt atgggtaagc tgtatttctc aacctggcaa aaaacctttt
     9001 actgtgtcaa tgcagaaaca ggcgagaagg aatgggaaag aaaagccgag ataacaacac
     9061 cggtaatttg ttcgggagac ctgctttttt acgggactgc cagcggaaag atagtctgtg
     9121 agagcattgc atcgaagaaa accatgtgga cattcgaaac gggccagaca atagcatttg
     9181 cttgcccgac aatctttgaa ggcaaagtat gtcttgggga ctggggcgga acacttcact
     9241 gccttgacca gaagacaggc agggaaacat ggtcattcaa ggctggcggg gcaatcaaga
     9301 gtgctgccgc attttccaac aattcacttt atttcggctg catggatgga acgttttact
     9361 gtctcaattc tgaaacaggc aaatcaaact ggacttttga ttcaggaagc ccagtcgaat
     9421 catctccagc agttggggcc gggagaatcg tgtttgcatc ttactacaac atacactgtc
     9481 tgaatgaaca gaccggcacg ccattatggt cattcaagcc taaattcagg gcgatctcca
     9541 gcccaataat tgcgggagac aaagtttacc aggccacact ctgccaatca ctccactgtt
     9601 tcgacctgga aaaggggacc gagttctggc attacgagga cttgataatc cacagcagtc
     9661 cctcatgcat gtctggcatg atatacattg gaaacgacca tgggacgctt tattgctttg
     9721 gcgatacaaa ggctggagga aaacaggaac ctctcttttc catcgagcca gccgagatgg
     9781 tctttgaaag gccaggcaca gaaaaacagc tgaaggtgac aaaccttact ggagattacc
     9841 tttctctaaa tgccaaaact ggcgaggcgt ggataaagct ctcaaaaaca gtatttgaac
     9901 tcgctccaca aaaaacagca tacataatgg tttctgttgc atgggaacta gggacagatg
     9961 aagaaatggc cggctacata accattgaga atgagacttg ggaagaaatt gcacgagtaa
    10021 tcgtaaagaa gaaaaatact gaagatttct gtcagtggca gatgttccgt ggaggccccg
    10081 aaaggaccgg ccacccggat ggtgaatgcg gcccgaaaga tccatcgtca atggaggcct
    10141 tgtggagttt tcaggcctgg cagggggccg tggaatcgtc tccggcaatc tggaacaact
    10201 cagtttactt tggcagttat aacaataatt tctattgtgt cgacattgac accggagaat
    10261 tgaaatggag atatgaatgc aaagatggta tctactcttc acctgcaata gatgacagca
    10321 aggtgtactt cggttccttt gacagttatt tcaggtgcct tgactgcaaa gatggaaagg
    10381 aagtcttcaa attcaagact ggtgacagaa tccattcatc accactggtt tcatctggaa
    10441 aggccatctt tggctcaaat gatggaaaag tctactgcct agatccaaaa acaggcgatc
    10501 agaaatgggt ttttgaggca aacactccca tacagtcttc gccagcccta aaaaatggcc
    10561 ggattttcat cggtgggttg aacggcacca tgtactgtct ggactttgaa accggaaaca
    10621 tcatctggga atacaatgca ggtggcagca taacgtgtgt tccagccctt gcctacgaca
    10681 gggtatactt tgggacatgg gccagaaaat tctgttgcct tgcccaggaa gacggcaaac
    10741 tccagtggtc atttgataca aaagagtaca tagaatcttc ggccgcatgt tcatccggaa
    10801 gggtctattt cggcgcaaat gacaattact tgcactgcct tgacgggctg acaggtgatg
    10861 aaatttggac gttcaggtcg caggacagaa aagtcacatc ttcgcctgca attgattcaa
    10921 acgaaaaaat atatgtcggg acctgagatg gtaatcttta ctgcctggat ggcagaaacg
    10981 gagaaaagat attcgaattc cagacacagg gtgtagttag aagctcagcc gcaatatcag
    11041 ggggaaagat cgttttcggc tcacatgacg gaaaggccta ctgtctaacc aacaaaacaa
    11101 aaaaccatta aaaacaaaaa ggccagctct tcagctggcc tttttaattg tggcagatta
    11161 tttttttatg gggatattct gggtatctgt caatgcacct gaaaacctga aagttattgc
    11221 gtctggatga ttttcgagga gtttgaagtt gtagatcttt ttggctttgg gctgaagggc
    11281 aaatggtttt aaatcaattt caaggccagt tgcctgtcta gcttcaatga caactctctt
    11341 tttaagatta tttgtcacca caatctggcc ttcattcgta ataatttcgc tggggttaaa
    11401 gctcagcccc gactcaacat ttacgacaac aggataattg acttcaatgc caccgccttt
    11461 tatgtccaga gaggtaggaa aagagcctgc tgaaactgcc gtggatgaag tctttatcac
    11521 aacaactctc atgcttctgg agggaatttc aagtgttggc ggatagacaa gaacccatgg
    11581 atcttttggc attatatcaa cattcaaatc aatttcactg gggtttaaaa ccataatcgt
    11641 cattacctgt cccaatccaa tataggcaga acgaggcacg aactcgagtc ttgggtctga
    11701 ttcgtaaacc ttcagagaaa cgttgagcat tgccgaagca ccaccagacg ggtcatcggt
    11761 gacctcatta acgtatatct gcgccacatg catgcctggt ttcatatctt tggtcgaaca
    11821 tgtcagtttg agctcagaag gttgcctcac aagcactttc ccttccttgt gatcaagctg
    11881 gagccaatca gcgctggatt ctactgtata atcctgatca aatgtagttc cattaataat
    11941 gtctaccttt gagatgactg tcgagtcggc ttttgcagca agctgaacct cggaaggaga
    12001 caagactatc aggcccctcg catagacagt gcccactacc aggagcgcca gaagagatac
    12061 agttacaatg attttgcgtt tcatactacc cataatataa atttatatcg tggaaagttc
    12121 attgcaacaa gtgatgcttg gacaaattcc agtttcttgc aatgttctac tagtaaacct
    12181 gtcactttga aaaaccaaaa ataaaaattt gttttttggc atcgatggca accatgtagt
    12241 caaaaccaaa tgctagatca acaccaacgc cagagctgac ttttaacttc ttttttgtct
    12301 gcccgtttgt cgcatcaaga atgtacaaat caggggagtt tccagaagca atgaggaggt
    12361 tcccagattt tgcaagctgg ttccaaaaaa tgaaatcact ttttttatcc aacacactct
    12421 tccagacaag ctttccatca tcagcaccat agcaccagat tataccattt cctggcaccc
    12481 agactttgcc atcagcaaca gtgggtgcgg cgccagaata gcccctttct gcctgccaaa
    12541 ccgtctctcc agcccaaagg gacaggcaca ttatcttttc atcaagggta ccgacataaa
    12601 ctttgtcgcc gaaaataacc ggaacaccag caaatccagt ttcaagctta cgactccaaa
    12661 ctttgtttcc attgccaagc aagaaacatg agagctcgtt ctcgctattg acaagaacaa
    12721 aagtgccttg gcaagacaaa ggctgaacgt taccagaaat cgctttctcc cagaccctga
    12781 taccagtttc caggtcgacg cacaccacgc ttttcttgcc agcaaaaaca cccagtctgt
    12841 caccaacaac ggtgatgtct gtttgccctt cctcgaccat ttttatgtcc caaactatgg
    12901 agccgttgtt ttctttcaat tttgcaagct tgccattttg tttgagcaca aacaggtgac
    12961 cccttgaaaa tgcggggacg cctattggct tggagtcaag tcttgtagcc cacacggcag
    13021 ctccccattt tgccagcgtg aaaacagaac catttgtacc agagcatatc acaaggtcac
    13081 catatgttga aacgccctgt atggtttctt ttgccttgat ggcttcacca ctcatgacaa
    13141 aattagggcc cgattcccag ggaatggcac cagtttttag ggggtcgtag cctctttggc
    13201 tccagatagc cctttctggt ggaaaagctg cagttactgc aagaggagaa agaagaactg
    13261 acagcgcaag aaaaaaagac aaaactctct gcatggaaat atattctatt cgactgggat
    13321 gtatcctcaa gatagcaaag gcaaccttat aaagaaagtg gcgcctgctc cttcactgct
    13381 tgaaaatgac acggaaccac catgcgattc cacatgcatc ttgactatgg aaagccccat
    13441 ccccatacca tttttcagat ctccttcagg attgacctgg tagaatggtt taaaaataaa
    13501 attctgtttt gattttggta tgccaatgcc attgtcggca aaacttgcca agagggtttc
    13561 accttcacat tttgcttcca gttttatctt aagagactgc ccttctc
//
LOCUS       DAOSRI010000005        13255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4731_length_13255_cov_4.286136, whole genome shotgun sequence.
ACCESSION   DAOSRI010000005 DAOSRI010000000
VERSION     DAOSRI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_13255_cov_4.286136"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PK717_00305"
     CDS             complement(<1..1000)
                     /locus_tag="PK717_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46147.1"
                     /translation="MVWCDYPTNPTCDLVKLWTYTYNDYGDLKSIIKGCEFQGTTYTY
                     DYEVEGSPSHTGQVAKVQYDAPLNKQRPETTYEYTDQCGNMMKSKNGNGSETTYEYYD
                     NGLPRRVNHPTGETREIEYDASNQPIKLKSSSCGSGTIETTMTWTDDGKPLGINYPTG
                     ESYNFIYDQKDRLSKVTGNYPAEFSYNTKGQISQIVEGPTNHTVTSSFNYDTKGRVSS
                     ITKDSQTSSYEYLSETGLLKKVTDPQGKETNIYYNDDSTVKKIIHPGNRTEEWLKDQN
                     GRINRYVPPDGHLITLEYTDNGRINKVSWEDGYYLTATYDDWGRPTRLTDPNGNHQD"
     gene            complement(1005..3101)
                     /locus_tag="PK717_00310"
     CDS             complement(1005..3101)
                     /locus_tag="PK717_00310"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HOR46148.1"
                     /translation="MRKFVVPAMAIILMFSQVSFVVSKDNGDDSKTQSQTAHAVPPST
                     LSQSIYNLSSINSGVLASSTFYVPDKNSGWEKIDDNGFKIGAGQQVKLLSPRYKGPSK
                     VKFSDDDNIFVRVGTCQIGDITNFCDDSITYEKYFQPAGSVDLSKAARETEQNKNAGI
                     IVSITNASGREAIIKPGPAKNEFGKIYTLTIDPMKSMTTSPVGASIPSVVDFASLVND
                     NGQVTKYVLPNSRFDVTNYIVQEPPQSIVSNTYINYEFGNLQNVYITEATLDFSNIIN
                     SCVSTNVGILNIGTNWNGQDINWENQPQGDLIDTFQCPSRCGIVDITAAVREASGTST
                     NIGLGFKQQDIASTLCIVNQPLPVMAVNYVYYEPDDTLGSYIQANWNGDCLGNDIGKE
                     HQTFYEELNDGNHLLNTANLNFITDFSPFSLPVKGSSALSMNFSITYNSQSYIDTGIG
                     WGWVHNYQVKLTPWCEDYLILRGSTGTLTRLASQGTTPRSWKAEKSHLMAEEFFDEEN
                     NPWIRLHHKDGTKIEFKLMPNGNYLPVKYLDCQGRYFYLYYSPEGLLTNVRNDYSSRN
                     LEFFYLPGTTRLVKVRDNTTDPTDCEFEYGTYPSGLTYLKNIKLNGVYVYENIEYTQL
                     GEMKPKGLNIQYKQGEPGKVEFTDHHIPNNQEESVQRWHWQIHHSQDCHYFYDQYTTK
                     TTEDNVLGIGYKGGSA"
     gene            3540..6404
                     /gene="cas3"
                     /locus_tag="PK717_00315"
     CDS             3540..6404
                     /gene="cas3"
                     /locus_tag="PK717_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOR46149.1"
                     /translation="MKKSLLFWGKTLQEKDTTNSGVVIAKSHPLLFHMVDVASVTEKI
                     FERAISDHIKKNLKTLYQNEDDSSLSSKIAFLAGLHDLGKATPPFQGLWREGAKELSA
                     EGLKFPPVNDKTKNHGLMTSSLIFAEFESTKDKDKLTNQIGRKIAISLGGHHGLMSDC
                     YVWKDFASHKFFSGSASWLEARKELLSDYYKCIYEMDMGEKTLKEAILEALPKDESLK
                     ILQSNDSFFAFLSGLVSVADWVGSSTDWFEFHEPDDSTSAIDDYLSHSRESAARAVDN
                     VWPEETGEQKMFANGEELFSYIFPEIRQGPRPLQRKCIDIAPTSSDGKMDSNQIVIIE
                     APTGEGKTEAAFYLFDSWQRQNVAHGAYIALPTMATSNQMFSRFKNFLSRGHGEKITL
                     NLLHSMKFFNDDFRELKIVGANDQGYSDTIVANSWFQKSKRGLITPYAVGTIDQSLMG
                     ALQVKHMFVRLFGLAGKTVIFDEVHAYDTYMSNLLDMLISWLSELGSSVIILSATLPE
                     KRKRELLKAFCRDSVKGEAKNTYPAITYTSKNKKIESATFQSNKEVRPVVTLKKAGSS
                     IQETADTIMDLIDQGGCAAWICNTVNQSQEAFHYLKEKAPADVELVLFHARFTASDRL
                     GIEDRVLSMMGKGQQAKRPKKAIVVATQVIEQSLDLDFDVMVSEFAPIDLLVQRVGRL
                     HRHKREQRPPKVSEPAMFVQFPEKKDGIPDFGPSKYIYEEYVLAKTLLTLENRLKEDS
                     YTFSDRDDTVKKLIEEVYGDSGSDAMELEGHLKKLKKKFDEKNQEIESNARKIIIDYP
                     VQIPAGKKQADDFRFAFINYYRDCKAPDDDDPGNPTKFAPLTRLSDGPSFKAVCLFED
                     GGKTYLDLETKQPVDISKSPKNIHETNSLIQNSLTISGWKNFNILKSLAKPESWANSP
                     SLKGMPVIIFKKTDGVWVSVVDTKFRYSREFGLEIAKEGD"
     gene            6404..8107
                     /gene="casA"
                     /locus_tag="PK717_00320"
     CDS             6404..8107
                     /gene="casA"
                     /locus_tag="PK717_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HOR46150.1"
                     /translation="METKEFNLQDEPWIWCTLNGKSKELSSKDVFKMSQNIGEITDIS
                     PMVTISIYRFLLAIMYSAFRGERRWPGKHEIWSIDDWKNIHADPDGAIEQILAYLETV
                     HERMWLFHPEYPFYQKPDLKLKESKDPEDGKMPIGKMRMYAANSSQGTLFDHTVESDL
                     FSTKSVYPDEAARLLLAFQNFDIGTAGTAEYHDDGQETKYLKDSLLRGEAVNFWQGKS
                     LFQTLLLNMIPVHELDALSKLGYPAGDSSDRPSFEVDPGHFWKEPGCRQKTEKGDFVY
                     IKKVEGIIDYLTWQSRKTKLFRNEQSMTVSFARTKGLVFPGNENMLDPFVIYNYREGK
                     KPSESGWKSKIRLDPEKQVWRSLDVLLARTIPSKHDFLPALTTKFLSETYSKEISNGD
                     IKLSLFCHYQEANQRIKDWSQQTFPIPATYMKNESFVDGIEIVLNIGKEISWGLKKSS
                     QTIIEELLAGKEKSVQIDLESDFWPLLQIPFARFVNDLGQYDEDAFWDDASQLKIREI
                     FSNWQNTVIALAKDVFNRATTKIRFNANGINAIIKGQQVLERSIDKLKSKYEIDNGGE
                     Q"
     gene            8104..8679
                     /gene="casB"
                     /locus_tag="PK717_00325"
     CDS             8104..8679
                     /gene="casB"
                     /locus_tag="PK717_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HOR46151.1"
                     /translation="MTEVNKKIHEKPAFVESLEDLVKKGDRAGLATLRKGLGKEPGTV
                     TEMYKYVYSRIPGQTNLFNEKIYFLTASLFALWHQGKQKLEKDFEGDFGKTFRQISKN
                     SESESIKERFTAIINSHFDDLPNHLRHAVSLAKSKDVPINWSFLLEDLKWWNNDDKRV
                     QRKWMKSFLQEEKKELPQAEGTQKEEEQNED"
     gene            8669..9808
                     /gene="cas7e"
                     /locus_tag="PK717_00330"
     CDS             8669..9808
                     /gene="cas7e"
                     /locus_tag="PK717_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOR46152.1"
                     /translation="MKIELHILQNFAPSNLNRDDTNAPKDCEFGGVRRARISSQCLKR
                     AIRTNEIFSNTLKSSDSEQDVSVGKRTRLLKDQIIAILGLQENLKDVQKTSDKEKTAK
                     TENSKKLDDFIKAAVKTAIDDSDATKMLIHYDDAEIKMLANLFSKVLSGEKKPEEAAK
                     EFKPKSKASDIALFGRMVAENTDFNVDAACQVAHAISTNKVSMEMDYFTAIDDLKKDF
                     ASEDAGAGMIGTVEFNSACFYRYSVIDTNQLRANLDGDNELSKKTVRAFIESSIAAIP
                     TGKQNSMAAQNPPSFVMAVVKDSCAPWSLANAFEKPVGPGQNGLVVSSIERFDDYWGR
                     LVSVYSDDGVKTAALFTTENVDLKNLVSSKVSGKKELVEKIIGAI"
     gene            9811..10596
                     /gene="cas5e"
                     /locus_tag="PK717_00335"
     CDS             9811..10596
                     /gene="cas5e"
                     /locus_tag="PK717_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOR46153.1"
                     /translation="MDSSNKFALILRLAGPMQAWGIQSRFEYRDTGAEPSRSGVIGLL
                     GCSLGVGREDKEFLSRCMRLEMGILVLKEGAVLWDYQVSGGGKWKGREYGVATANGKS
                     TTVVSNRHYLSDADFLVGLIGEPGFIEELSAGVKSPVWPIFLGRKAFVPSHPVFVATL
                     QLHDIKEALAEGLLRLATKEGWLKDSNSGNSSNPVLSNHPWLAGSKQEPPLLRMQIEC
                     DQDAGTEKNDVPLSFSKLDRKYMKRYVSNTYIDPAAILGKGKA"
     gene            10602..11258
                     /gene="cas6e"
                     /locus_tag="PK717_00340"
     CDS             10602..11258
                     /gene="cas6e"
                     /locus_tag="PK717_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOR46154.1"
                     /translation="MDEIYFSKIRPDFRNHDARNAMSDTEKLHKLLMSAFPDFGGNGR
                     EKTNLLFRVETDGYIIASSTVKPDWSFLGPGFMVESTNVLDLSTTQFAGADFAFRLVA
                     NPTYKKSGSKNVVPLREYYTQNFLDKYKMAKNPPTVFEWMERKANNSGFQIRQLSAVG
                     LNYRPDGSSRRSINFKMYLFEGQLRVVDVPLFARTLKTGIGREKAFGCGLLSIAPIKG
                     "
     gene            11263..12192
                     /gene="cas1e"
                     /locus_tag="PK717_00345"
     CDS             11263..12192
                     /gene="cas1e"
                     /locus_tag="PK717_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HOR46155.1"
                     /translation="MKDLHELSRFEDCLAHVYVEHCVVEQDAKSIAIFDLNGKTPIPV
                     ANLSVLFLGPGTKISHQAIKNLADSGCIVFWTGEEGVRFYACGVGKGRSSRNLLLQAK
                     LVSNPEARLKVVRKMYEKRFEELLDPSLTLRQIRGKEGARMRDTYAMQSAATGVPWSG
                     REYDPKKFDFSNPINRALTSANACLYGICHAAIVSAGYSPGLGFIHTGLQLSFVYDVA
                     DLYKTETAIPISFKVISEGDYEVERRVRIAMRDSFKATKLMGRIIADMKECLTVPDYI
                     SEIPETFDLDPQNFIWDPEEGVLSGGINYNSDL"
     gene            12226..12735
                     /locus_tag="PK717_00350"
     CDS             12226..12735
                     /locus_tag="PK717_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46156.1"
                     /translation="MKKMTTLIMLVLVMAMDGAVQFQRVTMRPHETKFWCDENTVINI
                     NWTVDAPSDTVEEGLFLIVYSEDEGLQYADYFDLLEMSEFVDDKGQGTFAVGGLQKAR
                     YTVMIMDYMLPEHRFNADITVVPETGEEEGHIIKLDEIRHASHQQYILKMKTILKTEK
                     TGMIPKASR"
     gene            12736..13089
                     /gene="cas2e"
                     /locus_tag="PK717_00355"
     CDS             12736..13089
                     /gene="cas2e"
                     /locus_tag="PK717_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HOR46157.1"
                     /translation="MVVMVLEKVPASLRGDLTRWLLELKTGVFVGNISALVREKLWEK
                     ACGGCKGGAAMMLFSASTEQGFDIRFWGKTSRLVQDFEGVKLITIPEKTQTKSAISEV
                     PGDEHNTGEADLEEI"
BASE COUNT         3928 a         2679 c         2919 g         3729 t
ORIGIN      
        1 ggtcctggtg gttgccatta gggtcagtca accttgttgg gcgcccccag tcatcatagg
       61 tggccgtcaa atagtatccg tcttcccaag agaccttgtt tattctgcca ttgtcagtgt
      121 actcaagagt aatcaggtgc ccatccggtg gtacatacct gtttatcctg ccgttctggt
      181 ccttgagcca ctcttcggtg cggtttcctg gatgaataat cttttttacg gtcgaatcat
      241 cattgtagta aatgttggtt tctttgcctt gagggtcggt gactttcttc agtagaccag
      301 tttcgctcaa atactcatag cttgaagtct ggctatcttt ggtgatcgat gacactctcc
      361 cttttgtatc ataattgaag ctggacgtaa cagtgtggtt tgttggacct tcaacaatct
      421 gtgaaatctg gcctttcgta ttgtatgaaa attcagcagg gtaattgccg gtgacttttg
      481 acaatctgtc cttctggtca taaatgaaat tgtagctctc tcccgttgga taattgattc
      541 ccaatggttt accatcatcg gtccaagtca ttgtggtttc aattgttcca ctaccgcaag
      601 agctcgattt cagctttatt ggctggtttg aagcgtcata ctcaatctct cttgtttcac
      661 ccgttggatg attgaccctt cttggcaatc cattgtcata atattcgtag gtggtttcag
      721 aaccattccc atttttagat ttcatcatgt ttccacattg atcagtatac tcgtatgtag
      781 tctccggtct ttgcttgttt aatggagcat catactggac ttttgcaacc tgacccgtgt
      841 gtgatggaga tccttcaacc tcatagtcat acgtatatgt tgtaccctga aattcacatc
      901 cttttatgat gctcttgaga tcaccataat cgttgtatgt gtaggtccaa agttttacca
      961 aatcacatgt aggattggtt ggataatcgc accagaccac ctcctcaagc ggatccccct
     1021 ttatatccta tgcccaatac attgtcctca gtcgtttttg ttgtgtattg atcataaaaa
     1081 taatggcaat cttgtgaatg gtgaatttgc cagtgccatc tttgcaccga ttcctcctgg
     1141 ttgttcggta tgtggtggtc tgtgaactct acctttcccg gttccccttg tttatattgg
     1201 atattcaaac cttttggctt catctctcca agctgagtgt attcaatgtt ttcataaaca
     1261 taaacgccat ttaattttat gtttttcaga taagtgagcc cactcggata ggttccatac
     1321 tcaaattcgc aatcagtcgg gtctgtcgta ttatctctca cttttaccaa tctggttgtc
     1381 ccagggagat agaaaaattc aagattcctg ctcgaataat cgtttcttac attggtcaaa
     1441 agaccctctg gagagtaata aagatagaaa tatcttccct ggcaatctag gtacttcact
     1501 ggcagatagt taccattggg cattaatttg aattcaatct ttgtaccatc cttatggtgt
     1561 aatcttatcc atgggttatt ctcctcatca aagaattctt cagccatgag gtgagatttt
     1621 tccgctttcc atgatctggg ggttgtgccc tgactggcaa gccttgtgag cgtgccagtt
     1681 gatccacgca atataagata atcctcacac catggggtca atttcacctg gtaattgtgt
     1741 acccatcccc atccaatacc tgtgtcgata taggattgtg aattatacgt aatcgagaaa
     1801 ttcatcgaca aagcagacga ccctttgacc ggcagcgaga atggactgaa atcagtaata
     1861 aaattcaggt tggcagtatt cagaagatgg ttcccatcat ttagctcttc ataaaatgtt
     1921 tggtgttctt tgccgatatc atttccaagg caatccccat tccagtttgc ctgtatatag
     1981 ctgccgagtg tatcatccgg ttcataatag acatagttga ccgccataac tggcaaaggt
     2041 tggtttacaa tacaaagtgt ggatgcaata tcttgttgct tgaaaccaag cccaatgttt
     2101 gttgaagtgc cgctggcttc tctgacagcg gctgttatat caacaatccc gcaccttgat
     2161 gggcactgga aagtatctat cagatctccc tggggctggt tttcccagtt tatgtcttgc
     2221 ccattccagt ttgtgccaat atttaatatc ccaacatttg tgctaacaca tgaattgata
     2281 atattggaaa aatcaagagt ggcctctgtt atgtaaacat tttgcaaatt gccaaactca
     2341 tagtttatgt atgtgttgga aacaatactc tgcggtggtt cctggacgat gtaatttgtc
     2401 acatcaaatc ttgaatttgg aaggacgtac tttgtaactt gaccattgtc attcacaagt
     2461 gatgcaaaat ctactactga tggaatactt gccccaactg gacttgtggt cattgacttc
     2521 atggggtcta tggttaaggt atatattttt ccaaattcat ttttggctgg tcctggtttg
     2581 atgatggctt ctcttcctga agcgttagta atactgacta taatgccagc gtttttgttt
     2641 tgttcggttt ctcttgctgc tttggagaga tcaacgctcc cggctggctg gaaatacttt
     2701 tcataagtga tcgagtcatc acagaaattt gtaatatcac caatttgaca tgtcccaact
     2761 ctcacaaaaa tattgtcatc atctgaaaat ttgactttcg atggtccctt gtatctaggg
     2821 ctgagaagtt ttacttgttg gccagcacct atcttgaaac cattatcatc aatcttttcc
     2881 cagccagaat tcttgtctgg cacatagaat gtgctagatg ccaaaacacc gctatttatt
     2941 gatgaaagat tgtaaatact ctggctcaat gtcgatggag gtactgcatg agctgtttgt
     3001 gattgtgttt ttgaatcatc gccgttgtct ttgctcacca caaaactaac ctgtgaaaac
     3061 atcagaatga tcgccatcgc cggaacaaca aatttgcgca tatcccaccc ccaatgttat
     3121 ttttatgaat aaacataaat ctgtttccca gtagcatcaa ttatcctgga ttaatcaata
     3181 atgaaccaaa tatcaattcg atgtctggat tttgttaatt atattttcgt aaaaaagatg
     3241 gtcgaaaaaa tgtatgatgg attttgaaat attgcacaat caaatgctat ccaacgctat
     3301 tttttggagg acatcatttt ttgtgtttat ggtttcgatc cttttgcctg ttttgatatt
     3361 ttcgaaaacc taagtgaagg gttgtgacaa aatgctgtca caggtcatgt gtataaatgg
     3421 gaaggctatc ttgaataatg gtttaatgtc ttgttgatta tatggacatg ttgatgaaaa
     3481 aggtgctata atatttttgt ataatattat ctataacatt taaatagtgg agacaaatcg
     3541 tgaaaaaatc attgcttttt tggggaaaaa ctcttcagga aaaagataca acaaatagtg
     3601 gtgttgtaat agcaaaaagt cacccattgc tcttccatat ggttgatgtc gccagtgtta
     3661 cagaaaagat atttgaaagg gcgatcagtg atcacataaa aaagaatttg aaaacactat
     3721 accaaaatga ggacgattct tctctatcct caaagattgc cttccttgca gggttgcatg
     3781 atttgggaaa agctaccccg ccatttcagg gtctttggcg tgaaggagca aaagaacttt
     3841 cagccgaggg cttgaagttc cctcctgtaa atgacaagac aaaaaatcac ggattgatga
     3901 cttcctcatt gatttttgca gaatttgagt ccacaaaaga caaggacaaa ctaacaaatc
     3961 aaattgggag gaagatagcc atatctcttg gcggtcatca cggactgatg agtgattgct
     4021 acgtctggaa agatttcgca agtcacaagt ttttctccgg atccgcatca tggctggagg
     4081 cacgcaaaga actgctgtcc gattactaca aatgcatcta tgagatggac atgggtgaaa
     4141 aaacactcaa ggaagcgata ctggaagccc tcccaaaaga tgaatcattg aaaatccttc
     4201 agtcaaatga ttcatttttt gcatttctct ccggccttgt ttctgttgcc gactgggttg
     4261 gttcatcaac cgattggttt gaatttcatg agcctgatga ctccacaagc gcaattgatg
     4321 attatctaag tcactccaga gagtcggctg caagggccgt tgacaacgtg tggcctgaag
     4381 aaaccggcga acaaaagatg tttgcgaatg gcgaagagct attttcatac atattcccag
     4441 agattagaca gggtccaagg ccacttcaga gaaagtgcat tgatattgcc cccacctcta
     4501 gtgatgggaa aatggattca aatcagatag taataattga agcaccaact ggtgaaggga
     4561 aaactgaggc agcattctac ctctttgatt cctggcagag acaaaatgtt gctcatggtg
     4621 cctatattgc tttgccgacc atggcaacat caaaccagat gttttcgcgt ttcaaaaatt
     4681 tcctctcgag gggtcatgga gaaaagatta ccttaaatct tttgcactca atgaaattct
     4741 ttaatgatga tttcagggag ctaaagatag ttggtgcaaa cgaccagggc tactctgata
     4801 caatcgttgc caattcatgg tttcaaaaat ccaagagggg gctcataaca ccttatgccg
     4861 tagggacaat cgaccagtcc ctaatgggtg cacttcaagt gaagcatatg tttgtgaggc
     4921 tttttgggct ggccgggaaa accgtaatct ttgatgaagt ccacgcctat gatacatata
     4981 tgagcaattt acttgacatg ctcataagct ggctttcaga gctgggttcg tcagtgataa
     5041 ttctttcagc aacccttcca gaaaaaagaa agagggagct attaaaagct ttttgtagag
     5101 atagtgtaaa gggagaggcc aaaaatactt atcctgcaat aacttatacc agcaaaaaca
     5161 agaaaattga gtctgccaca ttccagtcaa acaaggaagt aaggccggtt gtgacactca
     5221 agaaagctgg atccagcatc caggagactg ccgacacaat catggatcta atcgatcaag
     5281 gtggttgtgc ggcctggata tgcaacactg taaaccagtc tcaggaagct tttcactact
     5341 taaaagagaa agctcctgcc gatgtggaac ttgttctctt ccatgcaaga tttactgcct
     5401 cagacagact tggcattgaa gatcgcgtcc tttcaatgat gggcaagggg caacaggcaa
     5461 agaggcccaa aaaagcaatt gttgttgcaa cccaggtcat cgagcagagc cttgaccttg
     5521 atttcgatgt tatggtaagc gaatttgctc caatcgacct ccttgttcaa agggttggga
     5581 ggctccacag acacaaaagg gaacaaagac ctcctaaggt ttctgagcct gcgatgtttg
     5641 tccagtttcc agaaaagaaa gatggcatcc ccgatttcgg ccccagcaaa tacatttatg
     5701 aagagtatgt tctggcaaaa acattgctca cccttgagaa cagactgaaa gaggattctt
     5761 atactttctc tgacagagat gacactgtaa aaaaactgat cgaagaggtt tatggagatt
     5821 cagggtcaga tgccatggag cttgaaggac acctcaaaaa actcaaaaag aaatttgatg
     5881 agaaaaatca ggaaatagag tcaaatgcta gaaagataat tattgactac ccggtacaaa
     5941 ttccagccgg gaaaaaacaa gctgatgatt tcaggttcgc attcataaat tattacaggg
     6001 attgcaaggc tccggacgat gatgatccag gcaacccaac aaaatttgcc ccattaacca
     6061 ggctttccga tggtccatct tttaaagcgg tttgtctttt tgaggatgga ggaaaaacat
     6121 acctggatct tgaaacaaaa cagcctgttg atatttcaaa atctccgaag aacattcacg
     6181 aaacaaatag tctaattcaa aactctttaa caatttcagg ctggaagaat ttcaatatct
     6241 tgaaatctct tgctaaacct gaatcttggg ccaattcgcc ttccctaaaa gggatgccag
     6301 taataatttt caagaaaacc gatggagtat gggtttctgt tgtggataca aaattccggt
     6361 attccagaga attcgggctt gagattgcaa aggagggtga ttgatggaga caaaagaatt
     6421 caacctccaa gatgaaccct ggatctggtg cactctgaat ggaaagtcca aggagctgag
     6481 ttcgaaagat gtatttaaaa tgagccagaa cattggagaa atcacagata tctctccaat
     6541 ggttacaatc agcatctaca gatttcttct ggccattatg tactcagcat tcagagggga
     6601 gaggcgttgg cctggaaagc atgaaatctg gtcgattgat gactggaaaa acattcatgc
     6661 cgatcctgat ggcgcaattg agcaaatact tgcctacctt gaaactgtcc atgaaaggat
     6721 gtggcttttc cacccagaat atccattcta tcaaaaacct gacctgaaac taaaggaatc
     6781 aaaggacccg gaagatggca agatgccaat cggaaaaatg aggatgtatg ccgcaaacag
     6841 cagtcagggc acactttttg accacactgt cgaatcggac ttgttttcca caaaatctgt
     6901 ttatcctgat gaagcggcaa gactattgct tgcattccag aattttgaca taggcacggc
     6961 aggtactgca gagtatcatg atgatggcca agaaacaaaa tatctgaaag attcattgct
     7021 gagaggggag gcagtaaact tttggcaagg aaaatctctt tttcaaacac tcttgctgaa
     7081 catgattcca gtgcatgaac tagatgccct gtcaaagctt ggataccctg caggggattc
     7141 atccgatcgg cccagttttg aggttgatcc tggccatttc tggaaagaac cgggttgtag
     7201 acaaaaaact gaaaaaggtg attttgtata tataaaaaaa gttgaaggga taatcgacta
     7261 tctgacttgg caaagcagaa aaaccaagct tttcagaaat gagcaaagca tgacagtttc
     7321 atttgcaaga acaaaggggc tggtttttcc cggaaatgaa aatatgcttg atccatttgt
     7381 aatttacaat tacagggaag ggaaaaaacc tagcgaaagt ggctggaaaa gcaaaatacg
     7441 attggatcct gaaaaacagg tttggagaag tctggacgtt cttcttgccc gaacaatacc
     7501 atcaaaacat gattttctgc cggccctcac aacaaagttt ctctcagaga cttattcaaa
     7561 agaaatatca aatggggata taaaactctc cctgttttgt cattaccagg aagccaacca
     7621 gagaataaaa gactggtcac aacaaacctt tcccatacca gccacctaca tgaaaaacga
     7681 gagctttgta gatggcatcg aaattgtttt aaatattggc aaagaaatta gttggggctt
     7741 aaaaaaatca tcacaaacaa tcatagaaga gctcctcgct ggaaaagaaa aatcagttca
     7801 aattgatctt gaatccgatt tctggccact gctgcaaata ccttttgcaa ggtttgtaaa
     7861 tgatcttggc caatatgatg aagatgcctt ctgggatgat gcctcccagc taaaaatcag
     7921 ggagatcttt agcaactggc aaaatacggt catagcactg gcaaaggacg tttttaacag
     7981 ggccacaaca aaaataagat ttaacgcaaa tggcataaat gcaatcataa aaggccagca
     8041 agtgcttgag aggtcaatag ataaactgaa aagtaagtat gagattgaca acggaggcga
     8101 gcaatgacag aggtaaacaa aaagatccat gagaaacctg cttttgtgga atcgctggaa
     8161 gaccttgtga aaaaaggcga cagggcaggg cttgctacat taagaaaagg ccttggaaaa
     8221 gagccaggta cagtcacaga aatgtacaaa tatgtttatt ccagaatacc agggcagaca
     8281 aaccttttta atgagaagat ttattttttg acggcttccc tgtttgctct ctggcatcag
     8341 ggtaaacaga aactggaaaa agattttgaa ggggattttg ggaaaacttt taggcaaata
     8401 agcaaaaact ctgaatctga atcaataaaa gaacgtttta ctgcaatcat caattcacat
     8461 tttgacgatt tgccaaatca cttgcggcac gctgtgtcat tggcaaaatc aaaagatgtt
     8521 ccaattaatt ggagttttct tctcgaagac ctgaaatggt ggaacaacga tgacaagcgt
     8581 gttcaaagga aatggatgaa atctttcttg caggaagaaa aaaaggaact tccacaggca
     8641 gaaggaacac agaaagagga ggaacaaaat gaagattgag cttcacatat tgcagaattt
     8701 cgcgccatca aacctgaacc gtgacgacac caatgcccca aaggattgcg agtttggtgg
     8761 agtcaggcgt gcaaggattt ccagccagtg ccttaaaagg gcaatcagga caaatgagat
     8821 tttttcgaat accctgaaat ctagtgattc ggaacaggat gtatctgttg gaaagaggac
     8881 ccgcctcctg aaggatcaga tcattgcaat acttggcttg caggaaaacc taaaagatgt
     8941 ccaaaaaacc tctgacaaag aaaaaacggc caaaaccgaa aactcaaaaa aacttgatga
     9001 ttttatcaag gctgcagtca aaacagctat tgatgacagc gatgcgacaa agatgcttat
     9061 tcactatgat gatgccgaaa taaagatgct tgcaaacctc ttctcaaaag ttctttccgg
     9121 tgaaaagaaa ccggaggaag cagcaaaaga gttcaaaccc aaatcaaagg cctccgatat
     9181 agccctcttt ggcaggatgg ttgcggaaaa tacagatttt aatgttgatg cggcctgcca
     9241 ggttgcccat gcgatttcca ccaacaaggt cagcatggag atggactatt tcactgccat
     9301 cgatgatctc aagaaagact ttgcctctga agatgccggt gcgggcatga taggtactgt
     9361 tgaattcaat tcagcatgct tttaccgtta ctcagtaatc gatactaatc agctcagggc
     9421 aaatcttgac ggggacaacg agctttccaa aaaaactgtc cgtgcattca ttgaatcttc
     9481 aattgctgca atcccgactg gcaaacaaaa cagcatggca gcacaaaatc ctcccagttt
     9541 tgtgatggcc gtcgtgaaag actcctgcgc tccgtggtct cttgcaaatg cgtttgaaaa
     9601 accggttggc cccggccaga atggtcttgt tgtttcatca atcgaacgct ttgatgacta
     9661 ctggggaagg cttgtgtctg tctactcgga tgatggtgtc aaaacagccg cccttttcac
     9721 aactgaaaat gttgatctca aaaatcttgt ttcctccaag gtttcgggga aaaaggaatt
     9781 ggttgaaaaa ataattggag ccatttagta atggattcct caaacaagtt cgccctgatt
     9841 ttaaggcttg ctggcccaat gcaggcctgg ggcatacaaa gccggttcga atacagggac
     9901 accggggccg agccttccag atcgggtgta ataggtctgc ttggctgctc tcttggagtt
     9961 ggcagggagg acaaagagtt cctttccaga tgcatgaggc tggagatggg tatacttgtc
    10021 ctcaaagaag gcgctgtttt gtgggattac caggtttctg gcggtggcaa atggaagggc
    10081 agggaatatg gtgtcgctac tgcaaatggg aaatcaacga cagttgtttc aaaccggcat
    10141 tacctttcag atgctgattt cctggttgga ttgataggag agccaggttt tatcgaggaa
    10201 ttgtctgccg gagtcaaatc gcctgtatgg ccaatctttc ttggaagaaa ggcttttgtg
    10261 ccgtctcatc cagtttttgt tgccaccctt cagctccatg acataaaaga agctcttgca
    10321 gagggccttt taaggttggc cacaaaagaa ggctggctga aggatagcaa ctctggaaac
    10381 tcatcaaatc ctgtcctttc aaaccatcct tggcttgctg gttccaaaca agaacctcct
    10441 cttttgagaa tgcaaattga atgcgatcag gacgctggaa ccgaaaaaaa tgatgtcccg
    10501 ctgagtttca gcaagctcga cagaaaatat atgaagcgtt atgtttccaa cacatatatt
    10561 gatccagcgg ccattcttgg caaaggaaag gcataaaaaa tatggacgag atttatttct
    10621 caaaaatcag gccggatttt cgcaatcacg atgcacgaaa tgcaatgtct gatactgaaa
    10681 aactgcataa attgcttatg tccgctttcc cagattttgg agggaacggc agggagaaaa
    10741 caaaccttct tttccgggtg gaaacagacg ggtacataat tgcttcatca actgtaaaac
    10801 cagactggtc ttttttaggt cccggattta tggttgagag tacaaatgtg cttgatcttt
    10861 ccacaaccca atttgctggt gccgattttg cctttcgtct tgttgcgaat ccaacataca
    10921 aaaaatctgg atcaaaaaat gttgtccctc ttcgcgagta ttacacgcag aattttctgg
    10981 ataagtacaa aatggcaaaa aatccgccaa ctgtctttga atggatggag cgaaaagcaa
    11041 ataattctgg ttttcaaatc cgccagcttt ctgcagttgg attgaattat cgtcctgatg
    11101 gttcatcaag acgttccata aacttcaaga tgtacctctt tgaggggcaa ttgcgggttg
    11161 ttgatgtccc cctttttgcc cgaaccttga aaactggaat aggcagagaa aaagcttttg
    11221 gttgtggcct tctttcaata gcgcccataa aaggttagaa aaataaaaga tcttcatgag
    11281 ctgagcaggt ttgaggattg cctcgcacat gtctatgtcg agcactgcgt tgtcgagcag
    11341 gacgcaaagt cgattgccat atttgatttg aatggcaaaa cgccaattcc tgttgccaac
    11401 ctttctgtcc tcttccttgg tccgggcaca aagataagtc atcaggccat aaaaaatctg
    11461 gctgactcag gatgtattgt tttctggact ggggaagagg gcgtaaggtt ctatgcttgc
    11521 ggtgttggca agggcaggtc ttcacgcaat ctccttcttc aggcgaagct tgtttcaaat
    11581 ccggaagcaa gactcaaggt tgtccgaaaa atgtatgaaa agcgttttga agaactgctt
    11641 gacccttccc tgacgcttcg gcagataagg ggtaaagagg gtgccagaat gagggataca
    11701 tacgccatgc aaagcgctgc aaccggtgtt ccctggtctg gccgggaata tgacccaaaa
    11761 aaatttgatt tttcaaaccc gatcaatcgt gccctgacct cagcaaatgc gtgcctttat
    11821 ggaatttgcc atgccgcaat cgtttctgct ggttactcgc ctggtcttgg tttcatccac
    11881 accggcctcc agctgtcttt tgtgtatgat gtggcagact tatacaaaac tgaaacggca
    11941 attcccattt ctttcaaggt tatctccgag ggagactatg aggttgagag gcgtgtcagg
    12001 attgcaatgc gcgattcctt caaggcgaca aaactgatgg gtcgcattat tgcagacatg
    12061 aaagaatgtc ttactgttcc ggattacatt tccgaaatac ctgaaacttt tgatcttgac
    12121 ccccaaaact ttatttggga cccggaagag ggtgttcttt ctggtggaat aaactacaac
    12181 tcggacttgt aaaccatcat tttccattgt tctagggagg ttttgatgaa aaaaatgaca
    12241 acactaataa tgctggtctt ggttatggcc atggacggtg ccgttcaatt ccagagggtc
    12301 acaatgcgac ctcatgaaac aaagttttgg tgcgatgaga atacagtcat caatataaac
    12361 tggactgtag acgcaccatc ggatactgtt gaggagggcc tatttctgat tgtatattca
    12421 gaagatgagg gccttcaata tgcagattac tttgacctgc tagaaatgag tgagtttgtt
    12481 gatgataaag gtcagggtac attcgcagtt ggtggtttgc agaaagctag atatacagtc
    12541 atgattatgg actatatgtt gccagaacat cgctttaatg ctgatattac tgtagtccca
    12601 gaaacagggg aagaggaagg acacattata aagcttgatg aaatcagaca tgctagccat
    12661 cagcaataca ttttgaagat gaaaaccatt ttaaaaacag aaaaaacagg aatgattcca
    12721 aaggccagtc gataaatggt tgtaatggtc cttgaaaaag tccctgcatc gctcagggga
    12781 gatttgacca gatggctgct ggaactcaaa acaggggttt ttgtgggcaa tatatctgct
    12841 cttgtcaggg aaaaactctg ggaaaaagct tgtggaggct gcaagggtgg agctgcaatg
    12901 atgctttttt ccgcatccac cgaacaaggt tttgatatcc gtttttgggg aaaaaccagc
    12961 aggctggttc aggattttga aggtgtaaag ctcatcacga taccagaaaa aacacaaaca
    13021 aaaagcgcca tttctgaggt gccaggtgat gagcataaca ctggtgaagc tgacctcgaa
    13081 gaaatatagg tttttccaat tgtttatttg tttatgaaac aaatataggc caggacaggt
    13141 tttattactt aaacaagatt ttctttattt cagggtaggc tgcatatcat tttctttagt
    13201 ctcttcccca cacacgtggg ggtgtcccta tctcttccat ctcaagggta aagct
//
LOCUS       DAOSRI010000006        13010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4823_length_13010_cov_4.184948, whole genome shotgun sequence.
ACCESSION   DAOSRI010000006 DAOSRI010000000
VERSION     DAOSRI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4823_length_13010_cov_4.184948"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK717_00360"
     CDS             complement(<1..371)
                     /locus_tag="PK717_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR46158.1"
                     /translation="MKIGIIGAGSAGYVAALRAAQLGGEVYLFEKGEIGGTCLNVGCI
                     PTKALIAGVYHARQARESAILGVLASDVRIDLNRLQDHKESVVTANVRGIEYLLQKRG
                     VKLNRLDAQIVSPKEITAQGE"
     gene            complement(368..1111)
                     /locus_tag="PK717_00365"
     CDS             complement(368..1111)
                     /locus_tag="PK717_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR46159.1"
                     /translation="MKVAAIIPAYNEERGIADVLKAVTGASLPNEVIVVSDGSIDRTV
                     EVAKTFPVNVIEQVPNQGKAAAMLKGVENTDAEILVFVDADLVGLTPELVDSLIRPIV
                     EDKAEVTMGLFQDGKFSTDLAQMIFPFLTGQRAMKRSVWEGAKVKKDSQYGVEIYLTK
                     YMEKQNLRVENVLLKGCTQLFKEQKGQAFEGFGRRLKMTFDIAKALFTSTEEPKKTKK
                     HKKHVAGKLKPARVSRIKKHSTRTIGGTK"
     gene            complement(1125..2828)
                     /locus_tag="PK717_00370"
     CDS             complement(1125..2828)
                     /locus_tag="PK717_00370"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR46160.1"
                     /translation="MKLSTYMAPTLREVPSEAEARSHQLMLRAGYIRKLAAGVYTLLP
                     LGKRVLEKISAIVREEMDNIGCQEILMPAILPAELWQETGRWDLYGGEMFRLKDRKGR
                     DFCLGPTHEEIVTNHVRDEVKSYRQLPMMLYQIQTKYRDEIRPRFGVIRSREFLMKDM
                     YSFDSTIENFEISYKKAFNAYCRILERCGVTYVPVEADTGAIGGSSSHEFVVQADNGE
                     CKYTLCPACGYAANVEAAKAGRRKDLPQESDEEMKLVDTPNAKTIEEVAKFLGKEPTR
                     LVKSMIYKADDRFVMVCVRGDDEICEPKLKKAIGAKEVELASQEETEKITGTIVGFAG
                     PVGFKGTIVADLELLDSKGTACGGLSKDKHYVGVSHGRDWKHMAYADIRETKDGDPCP
                     KCGVTMRISTGLEVGHTFNLGTKYSKAMNANFLDEDGTLKPFIMGCYGMGISRTVASI
                     IEQHNDEDGIIWPVSVAPFHVALVPVNVKDEQVMIIANKLYDDLKKEGIQVIMDDRDA
                     QAGVKFKDADLMGFPLRITIGKKAKDGIVEIRDRKTKQSADRPAQEAVAYIKEFLREN
                     Q"
     gene            complement(2832..4373)
                     /locus_tag="PK717_00375"
     CDS             complement(2832..4373)
                     /locus_tag="PK717_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOR46161.1"
                     /translation="MEQDFELEYFLNVLPDAVKESLLRLERFDDLYEVVLDLGRPPEA
                     RFKDSTYQLSETPVSFFDLEFVISRTGSFDRDNRAGIERTLHRISAIMNRRGRIVGLT
                     CRYGRSIQGVIDTVKDYFSSGKSVLLLGKPGVGKTTLLREAARIMSVEMKKRVIVVDT
                     SNEIAGDGDIPHVGIGNARRMQVPTGVEQHTIMIQAVENHMPEVIIIDEIGTEEEATA
                     CRTIAERGVQLVGTAHGNSLPNLLINPTLSDLLGGVNSVILSDEEAFRRGTQKTVLER
                     TSPPTFQILIEIRDRGSFAIYKDIATTVDKYLREHKLFPETRVRDTDGNIIKTEEQLQ
                     ESEKKTEPLEEFFKIQGEPYEKIEDSAVVSIYPFGVSRDYLERAAKSLHIRVNISRRL
                     SESDIVLTTHSYESKKPQTILEAKSLEIPIYTIPQNSLSQIKRFLRSIKDSHPEESVD
                     QSTLEEAVETVLRENQSIKLPPAPSRVRKYQHKFAERFGLPSSSIGEEPNRSVVIHPP
                     QFKED"
     gene            complement(4419..7547)
                     /locus_tag="PK717_00380"
     CDS             complement(4419..7547)
                     /locus_tag="PK717_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46162.1"
                     /translation="MKRIACLIAIITIIAPYLPKPALAETTFMVSSPRTDTTTQYQIT
                     FTQDKPIKKGDKIKISFQATESGKMPDYEVLECNYDQNSNRRFNPGPEFLARCKDNQN
                     AQKFEKMYLAQYKPENPTLNQLKDFADSFIPGFLLPPLPKNPKQLEQAFGNVISKIYV
                     MGMPCQPTPDTQLVNAIAERSVTISCPVNVDENTAQKGIYIVIGEAFKLMCPATQGEY
                     AVEIATQGSTQKGKISIFSPIVSDPIFTIDPPYPEETSTWIFSFKTGGGGALSISSSS
                     ITLKLPKGFAKSQEGANWSIDLNGSYIVSGPNMRIAEYQNHEALIIMVPANIKESSNV
                     KITLKNINNPKEIGPVECSVWTSSDNTPVDFPKQEIVFTQSVTSNPPDASFMATATIR
                     LVSEGFFEGEEVMIVLPSSSSFLPGAELKINGNKCSRINFGEKTAQFVLPVSIEKGSY
                     MRISFGPVKNPQDSGEVVKLSIGQKTFEISWTVNKYEAKFAVSCNPRTANTPCSMKFI
                     IAPPENLAPEERQNIKIEGPWNRAMTPQDGEIPILVNEVQKTATFEAGTISVNLEKSL
                     ENRETVTITIPQMAGFASPDKTGTFTATIGAEKLSAELVLAKAPPMVSYKFLSADGKI
                     VKPNEKGWIIQPVKLVLVTSTGKADVRLKGFQKDEITSKEQDLSLEISESLISKGVVA
                     YAKDDRGESQAVPIPFSINVSPPKIKITSPEKTANQAFKLDVEINIEYIRVTNDGYLA
                     VWPKVTAGNIVTEMKTAVTDKIESATFTLSYDLSLAIGKNRIAVLAENQAGQKTTLES
                     SVEYIYEQVNFDLPNDQPINLKQGQNTIKAKTNPGATVKIGDKEYKADSNGNLSITIG
                     IEAGYNIIPVTVTTELSAKQSYQMILTGERIVTMVLNVPSMVIDGKATTLKVAPTNNF
                     KNEKTVPSFYNGITFIPLRDIANTMFAEVTFDDKTKQVTLTQKRPGGNRIIKIKLGSP
                     IAIIDGKETPLMKELLIAPVVIKGTSMVPLRFISESMGAQVGFEAPTKKITITWPDLT
                     RLPENKSVIKIK"
     gene            complement(7659..8342)
                     /locus_tag="PK717_00385"
     CDS             complement(7659..8342)
                     /locus_tag="PK717_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOR46163.1"
                     /translation="MVRVTYIGHSCVLIQSNGTQVVIDPYIEKNIKAILPPKGINPKL
                     ILVTHGHHDHLGDTVTLAKKCKAKVMATSELADFLASKGLDVLRSNFGGKIPFDFGWV
                     KVWPAMHTSSSEGVDFGLAASFVVSIGGKSIYHAGDTCLTSDMTLIGEDKPIDLACLP
                     IGGIYTMGIDDAVKAVNLIKPRVVLPIHYNTFDAIRVDPEEFSQKVEKLDVRTKPVVL
                     NPGEQLELT"
     gene            complement(8354..9874)
                     /locus_tag="PK717_00390"
     CDS             complement(8354..9874)
                     /locus_tag="PK717_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOR46164.1"
                     /translation="MAKNKNRISKQQHVEAPPPKPKNFIDRIFNLSHSGSDWKKEIIA
                     GITAFLGTAFLMLVVPKIFGIAYQQNGMIEETVAFATLIVVVLANLASGILTNLPYVL
                     APSVATTLAMMSVLTYMSDSPNTMFGLILIAGVIFLAITILPIGKHIIQSFPKGLRYS
                     AVLGLGLMLVLMGLQASGLFGSALGSSKIAEQLAGQVYNIFPTSHSINIFDTRLLIFL
                     IGLICVGYFIFTKAKHPYLWTILLTTIIGLFFISETFGGGVANSGWISPHPDFMNVFN
                     FKSGLIYLPDTSQWLSSNLLQPSNLFSIFGKTAIIGIGTVVMLIVRDIFSASGTLAGL
                     ATLEGRMGPNDNDFKNSHRALMVQGGSKVLSGVFGAAPVEYVPESSVGIAAGGKTGIT
                     AVVYAGCAILAIFLLPLFRSIPMVALAPVFIAIGLSFIKFAQEINWKDALESIPAIVV
                     IVMTMVTWNIAIALMWGIIIQLCLCVAVWKFEKTNAGLWTLGVVSLVGLVLFYIGQ"
     gene            complement(9849..10382)
                     /locus_tag="PK717_00395"
     CDS             complement(9849..10382)
                     /locus_tag="PK717_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOR46165.1"
                     /translation="MLRDFEGSSPKIAESAFVDPNAIIIGNATIAQKCSIWPGAIIRA
                     DINTITIGEMSNVQEFVSIHVESEVATKIGNGVTIGHSATLHGCEIDDFSLIGIGSII
                     LDRSKIGHHCMIGAGSLIPPGKTIPPRSLVMGSPGKIIRELSDEECKDLEIHAQKYFL
                     LSEKHKGEIHGKKQKQD"
     gene            complement(10424..11812)
                     /locus_tag="PK717_00400"
     CDS             complement(10424..11812)
                     /locus_tag="PK717_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46166.1"
                     /translation="MPVKCATPNLGNFTFHGISSNNELVVSNQNEIFWLTKNGVQNSI
                     PANKVFSTPGADRIYFMIDDKLMSTDMEQRKISEIEAMLPVDGPVEAAGKWLATQTSL
                     GTELFYDCRTEAKLEGVWSFSTCDNESFLLTNKVGAGAILLSKNGQVLGKSFLPGEFQ
                     YAGKHVLCIRPEGCELLSKENLSPLCKLPAMDSYMVQNGHIIGKNAQGKATFVFGNGT
                     LTKKQMPELNSDYFTQDNLIGFDGDGKYKAYSLPDFEPVQLPDILMKSEWYWFKEAGS
                     FIIVEGDGEECIAIDLETTTPFAKVPKPASASGEYLVYSAADQVVVHRINGEKTVPNC
                     ITTAVDNGIKHPKPRMISSQGDRCTLIYVNNHENTILSRIDTQTNSRIGEQNITGYTA
                     KIVFCPQESSVIPYSKIIDTIDSSSIENLRRNGHGRASLAFISENALSAVVVSPINGE
                     INEYSLIEKSSD"
     gene            12169..>13010
                     /locus_tag="PK717_00405"
     CDS             12169..>13010
                     /locus_tag="PK717_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOR46167.1"
                     /translation="MNQETVSFLKGKKISVLGMGRSGRAIAKLLSDVGAKVFCSDSGN
                     VEELESLGIPFETGGHSERVFTSDIIVVSPGIPMDSEVLVKARSLSIPLRGELDLAAE
                     LLPCSYAAVTGTSGKSTTTALIAHMLNKAGIKSRPVGNIGDALSNHVAEAQPDWVLSI
                     EVSSFQCELMETFHPKSAVFTNLSQDHLNRHASMEEYAFLKERVLARMDGEDLAVVNA
                     DDRWSSSLDGKTCANVASFSRKNGPNIEAYFDGKDIYIRGKKVSSLSDYQLVGDFNAL
                     NLMAA"
BASE COUNT         3072 a         2906 c         2946 g         4086 t
ORIGIN      
        1 ttctcgccct gtgcggtaat ttccttgggg gagacaatct gcgcatcaag cctgttgagt
       61 tttacccctc ttttttgcag gagatattcg atgcccctga cgtttgcagt gactacggac
      121 tctttgtggt cttgcaatct gttcaggtcg atccttacat cggacgccaa aacaccaagg
      181 atcgcagact cacgagcctg ccttgcgtga tagacgccag ctatcagagc ttttgtgggg
      241 atgcaaccga cattcaaaca tgttccacca atttcacctt tttcaaataa atagacttcg
      301 ccaccaagct gggccgccct cagggctgcc acgtaaccgg cagagcctgc accaataatt
      361 ccaatcttca tttcgttcct ccaatggttc gggtactgtg tttttttatt ctggaaactc
      421 tggcaggttt tagcttgcca gcaacatgct tcttgtgttt ttttgttttt tttggctctt
      481 ctgttgatgt gaaaagggcc ttggctatgt caaaggtcat cttcaacctt cttccaaaac
      541 cttcgaaagc ttgccccttc tgttccttga acaattgcgt gcaacccttg agcaaaacgt
      601 tttccaccct taggttttgt ttttccatgt atttggtcag gtaaatctcg accccgtact
      661 ggctgtcctt ttttacttta gcaccttccc atacggagcg cttcattgcc ctttgtcccg
      721 tgaggaaagg gaagatcatc tgggcgagat cggttgagaa ttttccatcc tggaaaagac
      781 ccattgtgac ctcagccttg tcttctacga taggccttat gagcgaatca acaagttctg
      841 gagttagtcc aaccagatcg gcatcaacaa agacaaggat ttctgcgtcc gtattttcaa
      901 cgcccttcag catagccgca gctttaccct ggtttgggac ctgttctatt acattaactg
      961 ggaaagtttt ggccacttcg acagtcctat cgatggagcc atctgagaca acgatcactt
     1021 catttggcaa gcttgcccct gtaactgctt tcaggacatc tgcgatgccc ctctcctcgt
     1081 tgtaggcggg gatgatcgct gcaactttca tgtgtttcct ccctttactg gttctccctt
     1141 agaaactctt ttatgtaagc caccgcttct tgggccggcc tatcagcgct ctgttttgtt
     1201 ttcctgtctc ttatctcaac gatgccatct tttgctttct tgccgatggt gatcctcagc
     1261 gggaacccca taagatcggc atccttgaat ttgacgcctg cctgggcgtc cctgtcgtcc
     1321 attattacct gtataccctc tttcttgagg tcatcgtaga gcttgttcgc aatgatcatt
     1381 acctgctcat cttttacgtt tacaggcaca agcgcaacat gaaatggtgc cacggagacc
     1441 ggccagatta tcccgtcctc gtcattgtgc tgttctatta ttgacgcaac tgttcttgaa
     1501 atacccatgc cgtaacaacc cataatgaat ggcttgagcg ttccgtcttc atcaaggaag
     1561 ttcgcgttca tggcctttga atatttggta ccaaggttga atgtatgtcc aacctctaaa
     1621 cctgttgaaa ttctcattgt gactccacat tttggacacg ggtcgccatc ttttgtttcc
     1681 cttatgtctg cgtaggccat atgcttccag tctcttccat gcgaaacgcc aacatagtgt
     1741 ttgtcttttg aaagtccgcc gcaggccgtt ccttttgaat ccaggagctc cagatctgca
     1801 acaatcgtac ccttgaagcc aacgggtccg gcaaaaccaa caatggtgcc tgttatcttc
     1861 tcggtttctt cctgagacgc cagttcgact tcctttgcac cgatggcctt cttgagtttt
     1921 ggctcgcaaa tttcatcgtc acccctgacg caaaccatga caaacctgtc atcggccttg
     1981 tatatcattg acttgacaag tcttgttggt tccttgccaa ggaactttgc cacttcttcg
     2041 atggtctttg cgtttggcgt gtcaacgagc ttcatctcct cgtcgctttc ctgtgggaga
     2101 tcttttcttc tgccggcttt tgcagcttca acatttgcgg cataaccgca agccgggcag
     2161 agcgtgtact tgcattcgcc attgtcggct tgcacaacga actcgtgcga gcttgagcca
     2221 cctatcgcac cagtgtctgc ttctactgga acataggtta cgccgcacct ttcaagtatc
     2281 ctgcagtagg cattgaaagc ctttttgtag ctaatttcga aattctctat ggtcgagtcg
     2341 aaagagtaca tgtctttcat aaggaactct ctcgagcgta taacaccaaa tcttggtctt
     2401 atctcgtctc tgtactttgt ctgaatctgg tagagcatca tgggtagttg cctatatgat
     2461 tttacttcgt ccctgacgtg gtttgtaaca atctcctcat gggttggtcc aaggcagaag
     2521 tccctgccct tgcgatcctt gagcctgaac atctctccgc catacagatc ccacctgcca
     2581 gtttcctgcc aaagttctgc tgggagtatt gctggcatga gtatctcctg gcaaccgatg
     2641 ttgtccatct cttctctaac tatcgccgag attttttcaa ggaccctttt gccaagtgga
     2701 agcagagtat agacgcctgc ggcgagcttt cttatatatc ctgccctcag catgagctgg
     2761 tggcttcttg cctctgcctc tgacggcact tccctgagcg tgggtgccat gtaagtggaa
     2821 agtttcattg gttaatcctc cttgaattgg ggtgggtgta tcacaacgct cctgtttggt
     2881 tcttcgccaa tcgaggatga aggaagccca aacctttcag caaatttgtg ctggtatttt
     2941 cttaccctgg aaggtgctgg tggaagtttg attgactgat tttcgcgaag cacggtttca
     3001 acggcctctt ccagtgttga ctggtccacc gattcttccg ggtgcgaatc ttttatgctc
     3061 ctcaggaatc ttttgatctg cgagagggaa ttttgcggaa ttgtataaat gggtatttca
     3121 agtgatttgg cttcaaggat ggtctgtggt tttttacttt cgtaagaatg tgttgtaagg
     3181 acgatatccg attcagacaa ccttctggag atgtttactc ttatatggag cgatttggca
     3241 gccctttcca ggtagtccct ggaaacacca aatgggtaga tgcttacaac tgccgaatct
     3301 tctatctttt cgtagggctc tccctgaatt ttgaaaaatt cttccagagg ttctgttttt
     3361 ttctcgcttt cctggagctg ctcctcggtt ttgataatat tgccgtcagt gtcccttact
     3421 cttgtttccg ggaagagctt gtgttcccta aggtatttgt caaccgttgt ggcgatgtcc
     3481 ttgtatatgg cgaaacttcc tctgtctctg atctcgatga ggatctgaaa agttgggggt
     3541 gaagtgcgct ccagaactgt tttttgtgtt cctcttctga atgcttcctc atctgagagg
     3601 atgaccgaat tgacaccgcc tagtaggtca gatagagtcg ggtttataag gagatttggc
     3661 aagctgtttc cgtgtgctgt tccgacgagc tggacacctc tttcggcgat tgtcctgcag
     3721 gctgttgcct cttcttctgt gcctatttca tcgatgatga tgacctcggg catatggttt
     3781 tccaccgcct ggatcataat cgtatgttgc tccaccccag ttgggacctg cattcttctg
     3841 gcgttgccaa ttccgacatg tggaatatcg ccgtctcctg cgatctcgtt tgatgtatca
     3901 acgacgataa ctctcttctt catttctacc gacattattc ttgccgcctc cctgaggagg
     3961 gttgttttgc caacacccgg ttttccaaga agaaggacgg attttcctga actgaaataa
     4021 tctttgaccg tgtcaattac accctgaata gaacgaccat atctgcatgt gaggccgacg
     4081 atacgccccc tgcggttcat tatggcagag atcctgtgga gggttctctc gatgccggcc
     4141 ctgttgtccc tgtcaaatga ccctgtcctg ctgattacaa attcaagatc gaaaaaactt
     4201 accggtgttt cggacagctg gtatgtagag tctttgaatc tggcttctgg gggacgaccg
     4261 aggtccagaa caacctcata gaggtcatcg aacctttcaa gccggagcag tgactctttg
     4321 accgcatctg gaagaacatt caggaaatat tcaagttcga aatcttgttc cacaagtgag
     4381 atattaggag aaccccatga ttttgtaaat aaagccgatc atttgatctt gattacagat
     4441 ttgttttcag ggagtcttgt caaatctggc catgttattg ttatcttttt agttggtgct
     4501 tcaaacccga cttgtgcacc catggattca gatatgaagc gaagaggaac cattgaagtg
     4561 ccttttatta cgacaggagc tatcagtagt tccttcatca agggtgtttc ttttccgtcg
     4621 atgattgcta tcggacttcc cagctttatc ttgatgatcc tgtttccacc tggcctcttc
     4681 tgtgtgagag ttacctgttt tgtcttgtca tcgaatgtta cttctgcgaa catagtattg
     4741 gcgatgtctc taagcggaat aaatgttata ccgttgtaga atgatggaac tgttttttcg
     4801 tttttgaagt tgtttgttgg ggcaactttc agggtggtgg cctttccatc gatgaccatt
     4861 gatggaacat tcaacaccat tgtaacaatc ctttcgccgg tcagaatcat ctggtatgac
     4921 tgttttgcac tgagttctgt ggtgactgta acaggtataa tgttgtatcc tgcttcaata
     4981 ccgattgtta tagaaaggtt gccatttgaa tccgccttat attctttgtc tccaatcttt
     5041 actgtggcac ccgggtttgt ttttgccttg attgtattct gcccttgttt caggttgatc
     5101 ggctggtcat ttggcaaatc aaaatttacc tgctcataaa tatattcaac acttgactca
     5161 agagttgttt tttgcccggc ctggttttct gccaaaactg caatgcggtt tttgccgatt
     5221 gcgagcgaaa gatcgtagct caaggtgaag gttgcacttt ctattttatc ggttacagca
     5281 gttttcatct ctgtaacaat attgccagct gtcacttttg gccacactgc cagatagcca
     5341 tcgtttgtga cccttatgta ttcgatattt atttcaacat ccagtttgaa agcctgattc
     5401 gcggtttttt ccgggcttgt gatttttatt tttggtggcg aaacatttat tgagaatgga
     5461 attggaacag cctgtgattc tcctctgtcg tcttttgcat aggcaacaac acctttggag
     5521 atcagcgatt cacttatttc taatgaaaga tcctgttcct ttgaagttat ttcgtctttt
     5581 tggaaacctt ttagtctcac atcggctttt cctgttgatg tcacgagaac cagttttact
     5641 ggctggatta tccacccttt ttcgttcggt ttgactattt tcccatccgc agagagaaat
     5701 ttataggaaa ccattggggg tgcctttgcc agaacgagct ctgctgagag cttttctgct
     5761 ccaattgttg cagtaaaagt gccggtttta tctggagaag cgaaacctgc catctgtggg
     5821 attgttatgg taaccgtttc cctattttcc aaagattttt ccagattgac tgatatggtt
     5881 cctgcttcga aagttgccgt tttttgaact tcattgacca gtattggaat ctcgccatcc
     5941 tgtggcgtca ttgccctgtt ccatggacct tcgattttta tgttctgcct ttcctctggg
     6001 gcgaggtttt ctggaggtgc tatgataaat ttcattgaac atggggtgtt tgccgttcta
     6061 gggttacacg atactgcaaa cttggcctca tacttgttga cagtccatga tatttcaaat
     6121 gttttttgcc cgatggaaag tttgactacc tccccagagt cctggggatt tttcactggc
     6181 ccaaatgaga ttctcatgta tgagcccttt tcaattgaaa ctggcaagac aaattgggcg
     6241 gttttctcgc cgaaatttat tctgctgcat ttattaccat ttatttttaa ttctgcgcct
     6301 ggcaagaatg aagagcttga tggaagaaca atcattacct cttcaccttc aaaaaaccct
     6361 tccgaaacga gtctgatggt tgcagttgcc ataaaacttg catcgggagg attggaagta
     6421 acagattgag tgaaaactat ttcctgtttt ggaaaatcca ccggcgtatt gtctgaggat
     6481 gtccacacac tgcattcgac aggaccaatt tcttttggat tgttgatgtt cttcagggtt
     6541 atttttacat tggatgattc cttgatgttt gccgggacca tgattatgag tgcttcatgg
     6601 ttttggtatt ctgcaatcct catattgggg ccggaaacaa tataggaccc attgagatct
     6661 atactccagt ttgcaccctc ctgggatttt gcgaagcctt ttggcaattt caatgtaatt
     6721 gatgaagagc ttatcgaaag tgctccgcca ccgcctgttt tgaaagagaa tatccaggta
     6781 gaagtttctt ctgggtatgg cggatcgatt gtaaatatgg gatcagaaac tatggggcta
     6841 aatatcgata tcttcccttt ttgtgttgaa ccctgagtgg cgatttccac agcgtattcg
     6901 ccctgggttg cagggcacat caacttgaat gcttcgccaa taactatata aatccctttc
     6961 tgggcagtat tttcatctac attgacaggg caagaaatcg taacagagcg ctctgcgatg
     7021 gcattgacaa gctgtgtgtc tggtgttggt tggcatggca tgcccattac ataaattttg
     7081 gaaatgacgt taccaaaggc ttgttcaagt tgttttgggt tttttggcaa cggtggcaga
     7141 aggaaacctg ggataaaaga atcagcaaag tccttgagct ggtttagggt tgggttctct
     7201 ggtttgtact gtgccagata cattttctcg aatttttgtg cattctggtt gtccttgcag
     7261 cgtgcaagaa attctggccc aggattgaat cttctgtttg aattctggtc gtagttgcat
     7321 tccaggactt cataatccgg catctttcct gactctgtgg cttggaagct gatttttatt
     7381 ttgtcgccct ttttgattgg tttgtcctgc gtaaaagtga tttgatactg tgttgtggtg
     7441 tcggtccttg gtgaactaac cataaaggtg gtctctgcca atgccggttt tggcaagtat
     7501 ggtgcaatga ttgtgataat ggcaatcaaa caggctattc ttttcatgat acctccatta
     7561 agaaagtacg gcttggcctt ttcaaagtca atgaacaaac cctcaaaaaa caaaaagccc
     7621 cctaaaaggg ggctttttgt tttttgtaca aattattttt acgttaattc cagctgttcg
     7681 ccaggattca ggacaactgg ttttgtcctt acatcaagtt tttcgacctt ctgggagaat
     7741 tcctccgggt caacccttat tgcgtcaaat gtattgtagt gtatcggtag aactactcgt
     7801 ggctttatga ggttgactgc ttttacagcg tcatcaatcc ccattgtata aataccacct
     7861 attggcagac aagctaaatc aatgggcttg tcttcaccaa tcagggtcat gtccgaggtc
     7921 aggcatgtat cgccagcgtg gtagattgat tttccgccga ttgagacaac aaagcttgcc
     7981 gcaaggccaa aatcgacccc ttctgatgat gacgtatgca ttgccggcca aacctttacc
     8041 catccaaaat caaacgggat tttgccacca aaatttgagc gtagaacatc caatcccttt
     8101 gaggcaagga aatctgccaa ttctgatgtt gccatcacct ttgccttgca tttttttgcc
     8161 agtgtgaccg tgtctccaag atgatcatga tggccatggg tcaccagaat cagcttgggg
     8221 tttatgccct ttggcggcag aatagcttta atgtttttct ctatgtaagg gtctatcact
     8281 acctgggtgc cattgctctg aataagcacg catgagtgcc ctatgtaagt tactcttacc
     8341 atttacccta tttctattgg cctatataga agaggaccaa gccaacgagt gaaaccactc
     8401 caagtgtcca gaggccagca tttgtttttt caaacttcca tacagccaca cagaggcaaa
     8461 gctgtatgat gatgccccac ataagtgcta tggcgatatt ccatgtgacc attgtcatga
     8521 cgatgacaac tatggctggt attgattcaa gcgcgtcctt ccagttgatt tcctgcgcaa
     8581 attttatgaa ggagaggcca attgctatga aaactggtgc caatgcaacc attggaatgg
     8641 acctgaacag tgggagaagg aatattgcaa gaattgcaca gccagcgtaa acaacagcag
     8701 tgattccggt ctttccgcct gctgcgatac ccactgatga ttctggaaca tattcgaccg
     8761 gtgcagctcc aaaaactcct gacaggacct ttgatccgcc ctggaccatc aatgcacggt
     8821 gggaattctt gaaatcgttg tcgtttggac ccatcctgcc ctcaagtgtt gcaagaccgg
     8881 cgagtgtccc tgaggcactg aatatgtccc tgacgatgag cattacaaca gttccgatgc
     8941 ctatgattgc cgtttttcca aatattgaga atagattgct tggctgcagg agattgctcg
     9001 ataaccactg tgaggtgtcc gggaggtata tgagcccaga tttgaaatta aaaacattca
     9061 taaaatctgg atgtggcgag atccatccac tatttgctac tcctccacca aatgtttcag
     9121 atataaagaa aagaccaata attgtggtca ggaggattgt ccagaggtat gggtgttttg
     9181 cttttgtaaa gataaaatat cccacgcata tcaaaccaat gaggaagata agcaatctgg
     9241 tgtcaaagat gttgatcgag tgggacgttg gaaagatatt ataaacctgt ccagcaagct
     9301 gttctgctat tttgctgctt ccaagggccg aaccaaatag acctgatgcc tgaagaccca
     9361 ttagaacaag catgagtccc agacccaaaa ctgcgctgta tctgagccct ttcgggaagg
     9421 actggatgat atgttttccg attggcagga ttgtaattgc caaaaagatt acgccagcaa
     9481 tgagtatgag tccgaacatt gtattgggtg aatctgacat gtatgtcaga acagacatca
     9541 tggcaagggt cgttgcaact gatggagcaa gtacatacgg tagatttgtt aggataccac
     9601 ttgcaaggtt tgcaaggacc accacaataa gagttgcaaa ggcaaccgtt tcctcaatca
     9661 tgccgttttg ctgataagct attccaaaaa tctttggaac gacaagcatg agaaatgctg
     9721 tgccaaggaa cgctgtgatg ccagcgataa tttctttttt ccagtcagag ccggaatgtg
     9781 aaaggttgaa aattctgtct ataaaatttt ttggttttgg tggtggtgct tcgacatgtt
     9841 gctgcttact aatcctgttt ttgttttttg ccatgaattt ctcctttgtg cttttctgaa
     9901 agcaagaagt atttttgtgc atgaatctcg aggtctttgc attcctcatc agatagttcc
     9961 ctgatgattt ttccaggact tcccataaca agtgaacggg gaggaattgt tttgccgggt
    10021 ggtatcagtg agccagcacc aatcatgcag tggtgaccaa tttttgacct gtccaggatg
    10081 atcgagccaa ttccaatcag ggaaaaatca tcgatctcgc agccatggag tgttgcactg
    10141 tggccaattg tgactccatt gccaattttt gttgcaactt ccgattcaac gtgtattgaa
    10201 acgaattctt gcacgtttga catctcgccg atggtgatgg tattgatgtc tgcccttatg
    10261 attgcgcctg gccatatcga acatttctgg gcaatagtgg cattgcctat gattattgcg
    10321 tttgggtcaa caaatgcgct ctcggctatc tttgggcttg aaccttcaaa atctcttagc
    10381 atctctggcc tcgggtcaga ttctaagcaa gggggttggc atgtcaatcg gatgactttt
    10441 cgatcaagga atattcgttt atttcgccgt ttatggggga aacaacaacg gcagataggg
    10501 cattctcaga tataaatgca agacttgccc tgccatgacc gtttcttctc aaattttcta
    10561 tggaagaaga gtcgatagta tctattattt tggaataggg tatcactgat gattcttgcg
    10621 gacagaaaac aatcttggca gtgtagccgg ttatgttttg ttcccctatt ctagagtttg
    10681 tctgggtgtc tatccttgag agtatcgtat tttcatggtt attaacatat atcagcgtgc
    10741 atctgtctcc ttgggaagag atcattcttg gtttcgggtg cttaattcca ttatcaacag
    10801 cggttgtgat acagtttggt actgtttttt cgccattgat cctgtgcact acaacctgat
    10861 ctgcagctga gtaaacaaga tattcgccag aagcactggc cggttttgga acttttgcaa
    10921 aaggtgttgt cgtttccaag tcgatggcga tacattcttc gccatcacct tcgacaatga
    10981 tgaaactgcc tgcctctttg aaccagtacc attctgattt cattaaaata tcaggcagct
    11041 gaactggctc aaagtctgga agagaatatg ccttgtattt gccgtcgcca tcaaaaccaa
    11101 tcaggttgtc ttgtgtaaag tagtcggagt ttagttccgg catttgtttt tttgtcagcg
    11161 ttccattgcc aaacacaaaa gtagctttac cctgtgcatt ttttccgatt atgtggccat
    11221 tctggaccat gtagctgtcc attgctggta atttgcaaag tggcgagagg ttttcttttg
    11281 aaagcagttc gcaaccttct ggccttatgc acaggacatg tttgcctgcg tactggaatt
    11341 ccccagggag aaacgatttg ccaagcactt ggccattttt tgaaagcagg attgcaccag
    11401 ctccaacttt attggtaagc aagaaacttt cattatcaca agtagagaaa gaccatactc
    11461 cttcgagttt cgcctctgtt ctgcaatcat agaaaagttc tgttccgagg ctagtttgtg
    11521 tggcgagcca ttttcctgcc gcctcaacag ggccgtcaac gggaagcatt gcttctattt
    11581 ccgatatctt gcgttgctcc atgtcggttg acatgagttt gtcgtcgatc atgaagtata
    11641 ttctgtcagc ccctggagtt gaaaagacct tattggctgg aatggagttt tgaacgccgt
    11701 tttttgttaa ccagaaaatc tcgttctggt tggacaccac cagttcattg ttgctcgaga
    11761 tgccatgaaa agtgaaattt ccaagattgg gtgttgcaca tttgacaggc attattggca
    11821 atgtgtatgc aaaaatgatt tgtgctgtca gcagggagac caagagagcc ttaaattttg
    11881 tagtgttaat ggcaactcct cctatttatc aattccagcc tattttcaag gccctttata
    11941 ttggcagaaa taaacatgtc aaggttgttt ctttatgcag agccacattc catattaaaa
    12001 atagcctaaa ccttatcaaa tatcatttat ataagaggca ttttggttta aaaagactac
    12061 aaatttgcaa attgatcctt gctttattgg gtcgaaattt ttaggagaag aattgaaagt
    12121 ttgccctggg gttgatttgc acccagcttg agattatcaa ttctaaatgt gaaccaagaa
    12181 acagtgtctt ttttgaaggg taaaaaaatt tcggtcctgg gcatggggcg ttctgggagg
    12241 gccattgcaa aactcctttc tgatgttggc gcaaaagtgt tttgctcgga ctcaggaaat
    12301 gtcgaggagc ttgaatctct tggtattcca tttgaaactg gaggccattc agagagggtt
    12361 tttacctctg acataattgt tgtttctccg gggattccta tggattctga ggtacttgtc
    12421 aaggcaagat cgctttcaat acccctcagg ggggagcttg atctggccgc agagcttctg
    12481 ccctgcagtt atgccgcggt gacaggtaca tccggcaaat caacaacaac cgccctgatt
    12541 gcccacatgc tgaacaaggc tgggataaaa agcaggccgg ttggaaacat aggtgatgcc
    12601 ctttcaaatc atgttgccga ggcccagccg gactgggtgc tctcaataga agtctcgtcc
    12661 ttccagtgtg aactgatgga aacctttcac ccaaaatcgg ctgtttttac aaacctatcg
    12721 caagaccacc tcaacaggca cgcaagcatg gaagaatatg cttttttgaa ggaaagggtg
    12781 ctcgcaagaa tggatggaga agaccttgct gtggtgaatg ccgatgacag gtggagctcc
    12841 tcgctggatg gaaaaacgtg tgccaatgtg gcttcatttt caagaaaaaa cggcccgaac
    12901 atcgaggcgt attttgatgg caaagacata tatataagag ggaaaaaggt ttcgtcccta
    12961 tcagattacc agcttgttgg agacttcaat gcgctcaatc tgatggcggc
//
LOCUS       DAOSRI010000007        12992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4825_length_12992_cov_3.814176, whole genome shotgun sequence.
ACCESSION   DAOSRI010000007 DAOSRI010000000
VERSION     DAOSRI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4825_length_12992_cov_3.814176"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK717_00410"
     CDS             complement(<1..213)
                     /locus_tag="PK717_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOR46168.1"
                     /translation="MEKRNAFIIGGILILAGIILFAAQVTGWRFDWGQIWPLILILIG
                     CSLLFRIDKDKGVIFPAIILIGIGKLF"
     gene            complement(227..580)
                     /locus_tag="PK717_00415"
     CDS             complement(227..580)
                     /locus_tag="PK717_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46169.1"
                     /translation="MKLFDQLLDECAREGSRVATPDGLALRALSLYDAHKSAKKRQRT
                     MWLWVLMITIPLIAVAISFFTWISGFDPFYLLRKMLSIDFVSYTGDFTIILMTFGMMV
                     TMFFRLFKMRNVINN"
     gene            complement(567..1127)
                     /locus_tag="PK717_00420"
     CDS             complement(567..1127)
                     /locus_tag="PK717_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR46170.1"
                     /translation="MTAEESDRALFSALVDEHQGYLYSFVLGMVGNRDDAEDITAKAF
                     LKAYLAFKSFKRNCSFKTWVTTVAINLVKNYRRDKHPTSSIEAESEQIGFEPMDASIL
                     PEDTTMRCENTDAIKRAISQLPINYRTFIVLKYLQDLSYEEIADTTGVPVTTVRNRIH
                     QGKEILKELFKKNGVSAPTEDGYEIV"
     gene            complement(1124..4300)
                     /gene="ileS"
                     /locus_tag="PK717_00425"
     CDS             complement(1124..4300)
                     /gene="ileS"
                     /locus_tag="PK717_00425"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR46171.1"
                     /translation="MFEELGEKSIVSEEMETLEFWKKNDIFKKSISQREGRPHYVFFE
                     GPPTANGMPGIHHCLSRIYKDTVCRYKAMEGFIVDRKAGWDTHGLPVELEVEKKLGLH
                     NKKEIEDYGIEAFIKKCKESVFTYEKEWVKLTERIAFWLDFEHQYMTLSNTYVESLWW
                     ILAQTWKKGLLYKGHKVVPYCPRCGTSLSSHEVAQGYQETKDPSITVKMEILDEPGTY
                     FLVWTTTPWTLPANVALAVKADATYVLAEKNGEKYILVEALAQKVLEKEYQIIKTLSG
                     KELVGKKYRPLYSFIRPDKNAHYVVAGDFVGTEDGTGIVHIAPAFGADDMEVARQNDL
                     PVIITIDDAGKFLPQIDPWKGIFVKDADPEIITEIKKRGLLYKSEKVTHTYPFCWRCD
                     SPLLYFAKPSWYVKMSQLRDKLLENNSQVNWYPQHIKEGRFGEWLREVKDWAISRERY
                     WGTPLPIWVCDSCGHQEAIESIKELREKSPETPENIELHRPYVDSITYKCPECGGTMK
                     RVPEVVDCWFDSGSMPYAQWHWPFENKEMFETHFPADYICEAIDQTRGWFYTLMAVST
                     ILFGVTPYKNVLCLELIVDENGQKMSKSRGNVLDPWLVLNNQGADALRWTIYTASPPW
                     TPSRLGVNTVTESFRQFILLLRNIYSFFSMYANIDGFDPTKQKVSLKERHYLDRWIIS
                     ELNTLVGYVDEEMKKFGITSATRAITAFTDTLANWYVRRSRRRFWKSESDQDKASAYH
                     TLYEVLVTLSKLLAPFTPYFAERLYQTLVVPYDKTAPISVHLCDLQKSDPSKIDKELE
                     RRMEFVRDAVSVGLKARKSIKIRVRQPLSKITIRATEPYQIEAINEFSELILDELNVK
                     KVETCNSMNSYCQIKVKPNLKTLGKRFGAKLTSVKEKIESEDGHAIISKLESDGKILL
                     NVDGANEELSRDDLLIERNSQEGTFVDSDGQIEVMLTTTLTPELEKEGLAREVIHAIQ
                     GMRKEAGLAIDDKIKTVVTGSNAICEAVHSLKDYVSKETLSQSISFALDKDPLLQREF
                     QIEGETLTVAIWLFAKGTGQSLRPERTA"
     gene            4438..5421
                     /locus_tag="PK717_00430"
     CDS             4438..5421
                     /locus_tag="PK717_00430"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR46172.1"
                     /translation="MRDNSIKVGVAFGGPSPEYEISLNSGQNVCKEIPDDKYAVYPVH
                     ITNEGFFQIADRPSSNSLAAFSQPEIPAHKACRYLKNFDVIFNTVHGPYGEDGNFQGF
                     LEVLGIPCTGSNTAASALAIDKMRARMIMKEHGIPVPESVYLHSPFDKIPFMPAVIKP
                     NAQGSSVGVTIAKSPVELSSAIELAFSYGNIVIAEEYLEGQEITCSVLQRKNSKAEAL
                     PVTLIVPKTSEFFDIKAKYEVGASDEITPAPIPENSFKLAQELAVRCHEALGCSGVTR
                     TDIFLMTDGSMKVIEINTLPGMTNTSLVPQAAKHVGISFGQLLDIMIEGAL"
     gene            5418..6584
                     /locus_tag="PK717_00435"
     CDS             5418..6584
                     /locus_tag="PK717_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46173.1"
                     /translation="MREKAITRATLGIILPLAIIAYLSNISPEDVYLRVCSVVDGKEV
                     CCDITLVDRGGKKTKIKSDGSDAVKLPVGQYQVSVESPGFEEISAIIDVSQSSHQHVN
                     FGVIQQGQMFGRLLNGATLKPITGLEVRMEGKEGKFFSRSDLDGVFSITVPPGQYMLF
                     IDNPMVNGYSSSYFFSTGQRISEEIYLLPANLSGIKLSDYYSYSLSAKRKGHVAGFPQ
                     DFLVRIDRNNDGTAVMRQSFTANDYGGELTFVYNGDQVWWSEGNDYKVFNPEGVQAAI
                     KAMKVNEDLFDFLNETSTDRRFSKTTNRGIEKANGVLCDKTDVSYADFDNWMGNNIFE
                     YTTWTMKEGKLRGMPTRIAGKMSGRDEQYHSYNIQFDISVTDVGKQFKIPEIKK"
     gene            6744..7202
                     /locus_tag="PK717_00440"
     CDS             6744..7202
                     /locus_tag="PK717_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46174.1"
                     /translation="MIDIPVYVEDGTAMVPAQLLAIESGLELKKDLGRWKFFDNLGNP
                     VVTFFDGFKRIEYKDTEKFLTRPATYNSGYLWVPAKDCAELLGLELTFDKETKKIIIQ
                     KPEVHASWVDRLTETVGTKNLVLSCLGIAIFFILMFFVLTPIRRGWRSFK"
     gene            7216..7893
                     /locus_tag="PK717_00445"
     CDS             7216..7893
                     /locus_tag="PK717_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOR46175.1"
                     /translation="MNSRNKILASFALGVLAGLVLSAKKGQRLPISHRFQEPLSQKFG
                     LAYSGVLAGKVDKKIREFSKRKPVFKNPALNRHLDEWILPGLSLYHVLLDEMVDQKAA
                     LQETERVLRAIVSQDKKMVLFKNAPSPFAILRAYVISKLKSSFPKGAWDIKIVSFEKN
                     VVAFDISKCFYQEMLDYFGSPELTKVFCALDEELATHFPKSITFKRKGTIGRGDKICD
                     FRYEYRQ"
     gene            7894..9495
                     /locus_tag="PK717_00450"
     CDS             7894..9495
                     /locus_tag="PK717_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR46176.1"
                     /translation="MVKLSFFGGQNEIGGNKILLEDGDTRLFLDFGQSFTAEGNFFDQ
                     PFLSPQNTDDLRLVGAIPDVDGLYKNAGYRTMYDDEGFVGVCGTGEPKKIMAVLVSHA
                     HTDHAGYLGIIRKDMKFYGSKITKTFLGRRRDISEGWMMKYDDDSFTGLDDGETFEVG
                     SAKIKHISVDHSIPGASAFIIEMGGLLIGYTGDLRLHGRRRELTEKFVKEAQNAKLDY
                     LFCEGTRVDDGRTADPELQAEEAHSLHSEEQVASKLETILDRAKGMVIYDASPADMDR
                     MDMVIRIASQKGRNVLIDAKKAYLVHGINLLSGLYPELEKFDNCSLLLSRKKERMPKS
                     ETTSKPRKGADPEKEARKDTLREADFNNPDIYIETKYNGRLPYEKEISSIFEEFPNNC
                     ILPRIVWGPALRDEIIRNQDNLLIYTSMGPLSMMHAGKGLKGTYVYGKAEPFNEEMEI
                     SFRKLENWINLFGMELDYAHTSGHANRRDLEYIVNKIAPRWLLPIHTCHAEIFDKFYK
                     SACHWPNGAKVELAKDGPRLIESEI"
     gene            9654..11297
                     /locus_tag="PK717_00455"
     CDS             9654..11297
                     /locus_tag="PK717_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOR46177.1"
                     /translation="MPLPQLREAILQNNGQLPPELISTPHKQFRSVGTQKILVLPLMF
                     EDLKPVLSLDIIRDIMLTGKNSIDKFYQASSYGKSFVSFGKPQVPDWMMMPSSLFSYS
                     DIEDLFKDGLETAKENGIDFTEFDEDENGYPDLVIFLWPGVSYTCGGYMPGNFSVDLD
                     NYTAISCCEDAGESRSSFPVIVMYHEIFHGMGDLFDLYDYNYKNVNAGGWDLMGEGVY
                     MGYCGLSAFSRWKAGWLDIETIDKPGTYMVDDLNGDGQHKAYRINMPGSKTEWLLVEN
                     RQKTGGDSFHQGVPNQGLVFWHVDDSRPYEHRFNTTKGALITPGICVLCPEPDDFYKQ
                     NSAYGSNYSRTSLTFQTPAKPLPYSLDPTAPMIKITNISKSGPTMTFDLDYERPIKSV
                     LVTQKEVSFGKVRKGEIVTSSINFKNAGRGNLVVRLNVYEADWLKIDRNSFIGNDEDI
                     IMTCNTKRLEIGTHKGSIEYSGKDVDGSIIVKVEVIPELGDVNADGYVNSKDVELMEK
                     AFGKTKDDLKFDPKCDLNEDGIINGFDLIILAKHMPITA"
     gene            complement(11374..>12992)
                     /locus_tag="PK717_00460"
     CDS             complement(11374..>12992)
                     /locus_tag="PK717_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HOR46178.1"
                     /translation="VGAAGSIYNPAGIQPLDINVSRNTCHSFDPSFELYQNLPHIAWT
                     DNTCVEYGDIYYVSWSTIVGNWVTAGGTTFNPLVGNANVSRNSGYSDQASLDIGSDGR
                     PHIAWTDDTYGLAGIFYVEYNPGYGNWMGANGSVYWPLASVQPFDIEVSRTLAASVQP
                     NLKLDFANRPYITWMDYSLSSNYGDTIVFVKWDNANSKWVGADNSPYITGANHHKNSP
                     GVIVSKSSGFTRAPMMQIDSAGTPHLTWFDNNYGGIYAIMYVTLGVPTEQTFQFQAKI
                     GYSLRDKTICNTAYFAGNTTNGSATSNECCIIATISENQPIIEVVKRARKSQAFSNEF
                     IVYTITVRNSGKLPSTDVTLYDVFPNNLQFVSSRPTGVISTTRAIFIVGVLSPGESRQ
                     YELVFKLKPGIVVPSSGLVITNIATATSKELEPVIDQASVLILPKEVSSKELYLWTSW
                     KGIDLKTNEGKIGGEIELSYKPQGGVSPYDVMVDWGDGSPKTSTNGLDGESVTTVKHT
                     YQTPGDYEVLIKCVDFQARTTIVRRKLRIK"
BASE COUNT         3615 a         2951 c         2928 g         3498 t
ORIGIN      
        1 aaataacttt ccaattccaa taagaataat ggcagggaaa atcacacctt tgtccttgtc
       61 aatcctgaac aggagtgagc aaccaattag tatcaatatc aatggccaga tttggcccca
      121 atcaaaacgc cagccagtta cctgggccgc aaacaggatt attcctgcaa gaataaggat
      181 accaccaatt ataaaagcat ttcttttctc catttggcac cttccctcaa ttgttaatta
      241 cattcctcat tttaaagaga cgaaagaaca tggtaaccat cattccaaac gtcataagaa
      301 taatcgtaaa atctccagta tatgaaacaa aatcaatgga cagcattttt cttaagagat
      361 agaacggatc aaaaccggaa atccatgtga aaaaagatat tgctacggct atgaggggaa
      421 ttgtaatcat caacacccaa agccacatgg ttctttgcct tttttttgca gacttgtgtg
      481 catcgtaaag ggagagcgcc cttagtgcga gcccatcggg tgttgcgacc ctgcttcctt
      541 ccctggcgca ctcatcaagc aactgatcaa acaatttcat atccgtcctc cgttggggcc
      601 gaaaccccat tttttttgaa aagttctttt aatatctctt tgccctgatg aatcctgttt
      661 cggacagttg tgacgggaac accggtcgta tcggctattt cctcgtatga aaggtcttgc
      721 aggtatttta ggacaataaa ggtcctgtaa ttgattggaa gctgtgagat ggcgcgtttg
      781 atagcatctg tgttttcaca cctcatagtc gtgtcttcgg gtaatatcga agcgtccatg
      841 ggctcaaaac cgatctgttc tgattcggct tcgattgatg aggtaggatg cttgtctctc
      901 ctgtagtttt tgacgagatt aatggccact gttgtgaccc atgttttaaa tgagcaattt
      961 ctcttaaaag atttgaatgc gagataggcc tttagaaaag cttttgcagt gatgtcctcg
     1021 gcatcatcac ggtttccgac cataccgagg acaaaagagt acagatagcc ctggtgttcg
     1081 tccaccaggg ccgaaaataa cgccctatca gattcttctg ccgtcaagca gtcctctctg
     1141 gtctcaagct ttgcccggtt cccttggcaa atagccaaat cgccacagtc agcgtttcgc
     1201 cttctatctg aaactccctt tgcagcaatg ggtccttgtc cagggcaaag gatattgatt
     1261 gagagagtgt ctccttcgat acgtagtctt ttagggaatg aactgcttcg catattgcat
     1321 tagaccctgt gaccactgtt ttgattttat catcaatggc aagcccggct tccttgcgca
     1381 taccctggat tgcgtgaatt acttcccttg caaggccctc cttttcaagc tctggggtga
     1441 gggttgttgt aagcatgacc tcgatttgtc cgtcagaatc aacaaatgta ccctcttggc
     1501 tgtttctttc aatcagaagg tcgtctcttg acagctcttc attggcacca tcgacattta
     1561 acaatatctt tccgtctgac tcgagcttcg agatgatggc atggccatct tcagattcta
     1621 tcttttcttt gaccgaggta agttttgcgc caaatctctt tccaagcgtc ttaaggtttg
     1681 gctttacctt tatctggcag tagctgttca tcgaattgca ggtttcaacc ttttttacat
     1741 tcagttcgtc aagtatcagc tccgagaatt catttattgc ctcaatctgg tatggttcgg
     1801 tcgccctgat tgttatcttg ctaagtggct gcctgaccct tattttgatg ctcttcctgg
     1861 ccttcaggcc aacagagacg gcatctctga caaattccat cctgcgctca agttctttgt
     1921 caatcttgct agggtcagat ttctggagat cacaaagatg taccgagatt ggcgctgttt
     1981 tgtcatatgg gaccacgagc gtctggtaga gcctctcggc aaaatatggg gtaaacggcg
     2041 ccaaaagctt tgacagggtg acaagcacct cgtaaagagt atgataggca gaagctttat
     2101 cttgatcaga ttcgctcttc cagaaccttc tccttgacct tctgacatac cagttggcaa
     2161 gggtgtcagt aaatgcggtt attgctcttg tagccgacgt gattccaaac ttcttcatct
     2221 cttcatcgac ataaccgaca agcgtgttga gctcggaaat tatccacctg tccagataat
     2281 gtctttcttt aagtgacact ttttgtttgg ttggatcaaa tccgtcgatg ttggcataca
     2341 ttgagaagaa ggaatatatg ttcctcaaaa ggagtatgaa ctgcctgaaa ctttcggtta
     2401 cggtgttcac gccaagcctc gaaggtgtcc acggcggaga cgccgtatag atggtccacc
     2461 tgagtgcatc ggccccctgg ttgttcagca caagccacgg gtcaaggaca ttgcccctgg
     2521 atttgctcat cttctgcccg ttctcgtcca caatcagttc aaggcacaaa acgtttttgt
     2581 atggtgttac gccaaaaagt atggttgaaa cggccataag cgtgtagaac catcctcttg
     2641 tctggtcaat ggcctcgcaa atgtagtcgg ccgggaaatg tgtttcaaac atttccttgt
     2701 tctcaaacgg ccagtgccac tgggcatagg gcatcgatcc actgtcaaac cagcaatcaa
     2761 caacctcagg cacccttttc atcgtgccac cgcattctgg gcatttatag gtgatagaat
     2821 cgacataggg cctgtggagt tcaatattct ctggcgtttc aggcgatttt tccctgagct
     2881 cttttatcga ttcgattgcc tcctggtggc cacaagaatc gcaaacccag atcggaagcg
     2941 gtgttcccca atacctttca cggcttattg cccagtcctt gacttccctg agccattcac
     3001 caaatctgcc ctctttgatg tgctggggat accagttcac ctgcgagttg ttttcaagga
     3061 gcttgtcgcg gagctggctc atcttgacgt accatgatgg ctttgcgaaa tagaggagtg
     3121 gtgagtcgca tctccagcag aacggataag tgtgcgtcac cttctctgat ttgtacagca
     3181 agcccctctt cttgatttcg gttatgattt ccgggtcagc atcctttacg aaaatacctt
     3241 tccagggatc gatctgtggc aggaatttcc cggcatcgtc aatcgtgatg attaccggaa
     3301 ggtcattttg cctggcaact tccatatcat cagcaccaaa agccggtgct atatggacaa
     3361 tgcctgttcc gtcctccgtg ccgacaaaat cgccggccac aacatagtgc gcgtttttgt
     3421 caggtcttat aaacgaatag agtggcctgt attttttgcc aacaagctct ttgccagaca
     3481 aagtttttat gatctgatat tccttctcaa gcaccttctg ggccaaggcc tcaaccagaa
     3541 tgtacttttc gccgtttttc tctgccagga cgtaagttgc atctgctttg actgcaagcg
     3601 caacatttgc cggcaatgtc cacggggttg ttgtccagac gaggaaatat gttccaggct
     3661 catcaaggat ttccattttc acggttattg acggatcttt ggtttcctgg tagccctggg
     3721 cgacctcatg tgaagacagc gatgtaccac atctcggaca ataaggcaca actttgtggc
     3781 ctttgtagag gagtcctttt ttccatgtct gggcaagtat ccaccaaagg gattcgacat
     3841 aggtgtttga aagggtcatg tactggtgtt caaaatccag ccagaacgct atcctttcgg
     3901 tcagttttac ccattccttc tcgtatgtga acaccgattc cttgcatttt ttgataaatg
     3961 cctcgatgcc gtaatcttca atctctttct tgttatgcag gccaagcttt ttctcaacct
     4021 caagctcgac gggcagtcca tgcgtgtccc agcccgcctt gcggtcaaca ataaaacctt
     4081 ccatggcctt atatctgcag actgtgtctt tgtaaatcct cgataggcaa tggtgaatgc
     4141 caggcattcc atttgccgtt ggagggcctt caaagaagac gtaatggggt ctgccttctc
     4201 tctggcttat cgatttcttg aagatgtcat tttttttcca gaactccagg gtctccatct
     4261 cctcggagac tatggatttt tcacctaact cctcaaacat gtttcctcca ggtattcatc
     4321 ttatccgtaa gattatatgt ttataagaat ttgtcgcaag ttaggagtgg tgcgggtgca
     4381 aaaacatgcc gcttggatat gggttgcaac ttgcaagctg gaatgtagaa tcatgtcatg
     4441 cgtgacaact cgatcaaggt tggggtggcc ttcggagggc caagcccaga atatgaaatt
     4501 tcgctcaact caggacaaaa tgtctgcaag gaaatacctg atgacaaata tgctgtctac
     4561 cctgtccata taacaaacga aggatttttc cagatagcgg acaggccttc gagcaattct
     4621 ctggcggctt tttcccagcc agaaatccct gcacacaaag cttgccgcta cctgaagaat
     4681 tttgatgtaa tcttcaacac tgtgcatggc ccttatggtg aagacggcaa tttccagggt
     4741 tttcttgaag tactggggat accatgcacc ggttccaaca cggccgcatc agcattggca
     4801 atcgacaaga tgagggcaag aatgatcatg aaagagcatg gaataccagt tcctgaatcc
     4861 gtctacctgc actctccatt tgacaaaatc cccttcatgc cagccgtcat aaaaccaaat
     4921 gctcagggat cttctgtcgg tgtcacaata gcaaaatcac ctgttgaatt aagcagtgca
     4981 atagaactgg ctttttctta cggaaacata gtgatagccg aagaatacct ggaagggcaa
     5041 gaaataacat gttctgtcct tcagagaaaa aactcaaaag ccgaggcact gcctgtcact
     5101 ctcatcgtcc caaaaacctc agagtttttt gacatcaagg ccaaatatga agttggtgca
     5161 tccgacgaaa taacgcctgc accaataccc gaaaactctt tcaagctggc tcaagaattg
     5221 gccgtcaggt gtcatgaagc acttggatgt tccggagtga caaggaccga catattttta
     5281 atgactgacg ggtcaatgaa agttatcgag attaacacgt taccgggaat gacaaacaca
     5341 tcgctggttc cacaggccgc caagcatgtg ggaatcagct ttgggcaact gcttgatatc
     5401 atgatagagg gagcgttgtg agggaaaaag caataaccag ggcaaccctc ggcataattc
     5461 taccccttgc aatcatagca tatctatcaa acataagtcc cgaagatgtt tatttgagag
     5521 tctgttcagt agtggatggc aaagaagttt gctgcgacat aacgcttgtc gatcgtggtg
     5581 gcaaaaaaac caagatcaaa agcgacggct ccgacgcggt aaagctccct gttggacaat
     5641 accaggtttc tgtggaatca ccaggctttg aggagatttc cgcaataatt gatgtaagcc
     5701 agagttccca ccagcatgta aattttggcg tcatacaaca ggggcagatg tttggcaggc
     5761 tgctcaacgg agcaacgctg aaacctataa ctggccttga agtcaggatg gaggggaaag
     5821 aaggcaagtt tttctcaaga tcagacctcg acggggtctt cagcataaca gtcccgcccg
     5881 gccagtacat gctgtttatt gacaacccga tggtaaatgg ctacagctcc agttattttt
     5941 tctctacagg gcaaaggatt tccgaagaaa tctatcttct gcctgccaat ctctctggaa
     6001 tcaaactctc cgattattac tcatactcgc ttagcgccaa gcgcaagggt catgttgcag
     6061 gtttcccaca agattttttg gtcagaatag atcggaataa tgatggaacg gcagtcatga
     6121 gacagtcgtt taccgccaat gattacggcg gagagctgac ctttgtctac aatggagacc
     6181 aggtctggtg gtctgaagga aatgattaca aagtattcaa tcctgaaggt gtccaggccg
     6241 caataaaggc aatgaaagtc aatgaagacc tttttgattt tttaaacgag acaagcacag
     6301 acagaagatt ttcaaaaacc accaatcgag gcatcgaaaa agccaatggc gtcctctgcg
     6361 ataagactga tgtatcatac gccgatttcg acaactggat gggcaacaac atttttgaat
     6421 acacaacctg gaccatgaaa gaaggcaaat tgagggggat gccaaccaga attgccggaa
     6481 aaatgagcgg aagggacgaa caatatcact catacaatat tcaatttgac atctcggtta
     6541 ccgatgttgg aaaacaattc aaaattcccg aaataaaaaa ataacctttc cgcttgattg
     6601 tttttgctta tcggataacc tattcgcata tgaggaagct cattccaatt ttgtgctttt
     6661 ttttgctatc ggtagaaccc gtatatgccg agatttcaat tgcaattggg gaaagaaagg
     6721 ttgaagtgaa aggcctttcc tgcatgattg acattcccgt gtatgtcgag gacggaacgg
     6781 caatggtccc tgcacaactc ctggcaatcg aatccggact ggagctgaaa aaagaccttg
     6841 gcagatggaa attttttgac aatctgggca acccggtcgt cacatttttt gacggattca
     6901 aacgaattga atacaaagac accgaaaagt tcctgacaag acctgccaca tacaactccg
     6961 gatacctctg ggttcctgca aaggattgtg cggaactgct tggacttgaa ctgacctttg
     7021 acaaggaaac aaaaaaaata ataatccaga aaccagaagt acatgcttcc tgggtagata
     7081 gactcacaga aactgttggg accaaaaatc tcgttctctc atgtttgggg atcgcaatat
     7141 ttttcatact gatgttcttt gtcctcaccc cgataaggcg ggggtggcgt tcttttaagt
     7201 aaaaggaggg ggctaatgaa ctcaaggaac aagattcttg ccagttttgc ccttggcgtc
     7261 cttgccggtc tggttctttc ggcaaaaaag gggcagaggt taccaatctc tcacaggttc
     7321 caggaaccac tctcgcaaaa attcgggctt gcgtattcgg gtgtactggc aggaaaggtc
     7381 gataaaaaaa tcagggaatt ttcaaaaagg aaaccagtct tcaaaaatcc cgccctgaac
     7441 agacacctcg acgaatggat attgccaggt ctttcgctct accacgttct ccttgatgaa
     7501 atggtcgacc agaaggcagc actccaggaa acagaaaggg tgttgagggc cattgtctcg
     7561 caggacaaaa aaatggtcct cttcaagaat gctccttcgc catttgcaat tctcagggcc
     7621 tacgttattt ccaagctcaa gagctccttc ccgaaaggcg catgggatat aaaaattgtc
     7681 tctttcgaaa aaaatgttgt tgcattcgac atttcaaagt gcttttatca ggagatgctc
     7741 gactatttcg gttcccctga gctgacaaaa gtcttctgcg cgctggatga agagttggcc
     7801 actcatttcc caaaatcaat caccttcaaa agaaaaggca ccattggcag gggtgacaaa
     7861 atctgcgatt tcaggtacga atacaggcag taaatggtaa aactttcctt ctttggtggc
     7921 caaaacgaaa ttggcggaaa caagatattg cttgaggacg gtgacacgcg ccttttcctg
     7981 gatttcgggc aatcattcac tgcagagggc aacttcttcg atcagccttt cctttccccg
     8041 caaaacacag atgacctgag gcttgtaggg gcgattccag atgttgacgg cctctacaaa
     8101 aatgccggct acagaacaat gtatgatgat gaaggttttg ttggcgtttg tggcaccggc
     8161 gaacccaaaa aaataatggc cgttctggtt tcacacgcac acaccgacca cgccggttat
     8221 cttggcatca tcaggaaaga catgaaattt tacggttcca agatcacaaa aaccttcctt
     8281 ggcaggagaa gggacatctc cgaaggctgg atgatgaagt atgatgacga ctcgttcaca
     8341 ggccttgacg acggggaaac atttgaagtc ggctctgcaa aaataaaaca catttctgtt
     8401 gaccactcga tcccaggtgc ttcagcgttc ataatcgaaa tgggcgggct cctgatcgga
     8461 tacaccggag acctcaggct ccacggaagg cgaagggagc tgaccgagaa atttgtcaaa
     8521 gaggctcaaa acgcaaaact ggactacctc ttttgcgaag gaacaagggt cgatgacggc
     8581 aggactgccg accctgaact gcaagccgag gaggcgcatt cactgcactc cgaggagcag
     8641 gtggcctcaa aactcgaaac catactggac agggcgaagg gcatggtcat atatgacgcg
     8701 tccccggccg acatggacag gatggacatg gtcataagga ttgcatccca aaaaggcaga
     8761 aatgtgttga ttgacgccaa gaaggcgtat cttgtccacg gcatcaacct gctttccggc
     8821 ctgtatccag agctggagaa atttgacaac tgctcactcc tgctgtccag aaaaaaagag
     8881 agaatgccaa aatctgaaac gacctcaaaa cctaggaaag gggccgatcc agaaaaagag
     8941 gcacgcaaag atacgctaag ggaagcagat ttcaacaatc cagacatcta catcgaaaca
     9001 aaatacaatg gacgtctccc atatgagaaa gaaatatcct ctatttttga ggagtttcca
     9061 aacaactgca tcctgccaag gatcgtctgg gggccagcgc tccgcgatga aataataagg
     9121 aaccaggaca acctcctgat ctacacaagc atgggaccac tctccatgat gcatgccgga
     9181 aagggactaa agggcactta tgtctacggc aaggcggaac cgttcaacga agaaatggaa
     9241 ataagcttca ggaagctcga aaactggatc aacctgttcg ggatggagct ggactatgcc
     9301 cacacatccg gccacgcaaa caggcgcgac cttgaatata ttgtcaacaa gattgcccca
     9361 aggtggttgc tcccaatcca cacatgccac gcagaaatat ttgacaaatt ctataaaagt
     9421 gcctgtcact ggcccaacgg ggcaaaggtg gagctggcca aagacgggcc aaggctgata
     9481 gaatcggaga tctaggtttt gttggttaca tttcgttaac tacgttttag tcttctcgtt
     9541 gtatagtttt gactatgaga gggaaacaaa cagtaacagc aatcatttgt ctcgtcgttg
     9601 tcttaattac accatcccat attgggaatg caaaaaaaat tgacatttca cacatgcctt
     9661 taccgcagct tagagaagca atccttcaaa ataatggcca gttgccacca gaacttatat
     9721 caacacccca caaacagttt aggagtgttg gcacacaaaa aatccttgtc cttcctttga
     9781 tgtttgaaga tcttaagcca gtattatcat tggatatcat cagggacata atgctgactg
     9841 gcaaaaattc gatagataaa ttttaccaag cctcttctta tggaaaatcc tttgtcagtt
     9901 ttggcaaacc gcaggtgcca gactggatga tgatgccatc gtcccttttt tcatattctg
     9961 atatagagga cttatttaaa gatgggttgg aaactgccaa ggaaaatggc attgatttca
    10021 cagagttcga tgaggatgaa aacgggtatc ctgatcttgt tatttttttg tggcctggcg
    10081 tttcatacac atgcggtggt tacatgccgg gcaacttttc agttgatctg gacaattaca
    10141 cagcaattag ttgctgcgag gatgcaggag aaagtagatc cagttttcca gtgatagtaa
    10201 tgtaccacga gatttttcat ggaatgggtg atcttttcga tctctatgat tataactaca
    10261 agaacgtaaa tgcaggcgga tgggacctga tgggtgaggg cgtctacatg ggttattgcg
    10321 gtcttagtgc gttttcaaga tggaaggctg gctggcttga tatagaaaca atagacaaac
    10381 ctggaaccta tatggttgat gatctgaatg gtgatggcca acacaaggca tacagaataa
    10441 acatgcctgg gtcaaaaaca gagtggttgc ttgttgaaaa tcggcaaaag acaggtggag
    10501 attcatttca ccagggagtc ccaaaccagg gtcttgtttt ctggcatgtg gatgatagca
    10561 gaccatatga acatcgcttt aacaccacaa aaggtgcgct tataacacca gggatatgtg
    10621 tcctttgccc agagccagat gatttttaca aacaaaactc ggcttatggt tcaaactatt
    10681 caagaacttc cctgacattc cagactccag cgaaaccatt gccatacagc ctagatccaa
    10741 ctgccccaat gattaagatt accaacatat caaaatcagg gccaacaatg acttttgatc
    10801 ttgattatga aagacctata aaatctgttc tcgtgaccca aaaagaggta tctttcggaa
    10861 aagttagaaa aggtgaaata gttacatcat ctataaattt caaaaatgca ggaagaggca
    10921 atcttgtagt aaggcttaat gtttacgagg ccgattggtt aaaaattgac agaaacagct
    10981 ttatcggcaa tgatgaggac attataatga cttgtaacac caagcggtta gaaattggaa
    11041 cacataaagg ttcgattgaa tattcaggca aagatgtcga tggaagcata attgtaaaag
    11101 ttgaagtcat cccagaactc ggcgatgtta atgctgatgg gtacgttaat tccaaagacg
    11161 ttgaattgat ggaaaaagct ttcggcaaaa caaaagatga ccttaagttt gacccaaaat
    11221 gtgatctgaa tgaggatgga ataattaatg gctttgattt aattatactg gcaaaacata
    11281 tgcccataac agcataacca atttcaatca cctgtattta attaaaaagg ccccttttca
    11341 ggggcctttt taattatttg aatacaataa agtctacttt atacggagct ttctcctgac
    11401 aatagttgtc ctggcctgga aatcaacgca ctttatcagg acctcgtagt ctcctggtgt
    11461 ctggtaggtg tgtttcaccg tggtcacaga ttccccgtca agaccgtttg ttgaagtttt
    11521 tggagagccg tcgccccaat caaccatcac gtcatagggt gaaactccac cctgcggttt
    11581 gtaggaaagc tcgatctctc caccgatttt gccctcgttt gttttcaggt ctattccctt
    11641 ccacgatgtc cagagataga gttcctttga agaaacctct tttggcaata taagcactga
    11701 ggcctggtca ataactggct caagctcttt ggaagtggcc gttgcgatat ttgtgatgac
    11761 aaggccggag gatggtacaa cgatccctgg cttgagtttg aagacaagct catactgtct
    11821 ggattcacct ggtgatagga caccaacaat aaagatggcc cttgttgtgg aaatgacacc
    11881 ggtcggtctg gacgagacaa actggaggtt gttcgggaaa acatcataaa gtgtgacgtc
    11941 tgttgaaggc agtttgccac tattcctgac agttattgta tatacaataa attcatttga
    12001 gaaggcctga gattttctgg ctctttttac tacttcgatt atcggctgat tctccgatat
    12061 agttgctatt atacaacatt cattgctggt tgccgaacca tttgttgtgt tgccagcaaa
    12121 atacgctgta ttacaaattg ttttgtccct gagtgaataa ccaattttgg cctggaattg
    12181 aaaagtctgt tcggtcggaa caccaagtgt tacatacatg attgcgtaaa taccgccgta
    12241 attattgtca aaccatgtga gatgcggtgt accagctgaa tcgatctgca tcattggtgc
    12301 tctagtgaaa ccactactct ttgagacaat gaccccaggg gagtttttgt ggtggtttgc
    12361 acctgtaatg tatggtgaat tgtctgcgcc aacccacttg ctgtttgcat tgtcccactt
    12421 aacaaacaca attgtgtcac cataatttga agagagtgag taatccatcc aagtgatgta
    12481 tggtctattt gcaaaatcca gtttgagatt cggttgcacc gatgcagcaa gcgttcttga
    12541 gacctctatg tcaaaaggct gaacagaagc aagcggccag taaaccgagc cattggcacc
    12601 catccagttt ccatatcctg gattgtactc cacatagaaa atccctgcca aaccatatgt
    12661 gtcatcagtc caagcaatat gaggccttcc atcagagcca atgtcaagcg aggcttgatc
    12721 agaatatcca gagttcctgc tcacattggc atttccaacc agtggattga aggtggtgcc
    12781 acctgccgtc acccaattgc caacaatagt actccaactg acataataaa tgtctccgta
    12841 ttccacacat gtattgtcag tccaggcaat atgtggaaga ttttgataca attcaaagga
    12901 tggatcgaaa gagtggcatg tatttctgct aacgttaatg tccaatggct ggattcctgc
    12961 tggattgtaa atagatcctg ctgcacccac cc
//
LOCUS       DAOSRI010000008        12885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4877_length_12885_cov_4.832658, whole genome shotgun sequence.
ACCESSION   DAOSRI010000008 DAOSRI010000000
VERSION     DAOSRI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4877_length_12885_cov_4.832658"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK717_00465"
     CDS             complement(<1..248)
                     /locus_tag="PK717_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR46179.1"
                     /translation="MRLIKRVSYIKNFGVFKDFDWGKSKLDDFKKNNFFYGWNYSGKT
                     TLSRVFASIGKTIENSGEWQIELDDGSKIKEKDSRPIN"
     gene            1126..1422
                     /locus_tag="PK717_00470"
     CDS             1126..1422
                     /locus_tag="PK717_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOR46180.1"
                     /translation="MYPVKLDVDYPEKLSRGILLLRTFFGVFYVMIPHGFCLFFYGIA
                     AMFVQFIAWWAILFTGKYPKGMFDFVVGYYRWSMRVNGYMGYMTDEYPPFNGRP"
     gene            complement(1500..4889)
                     /locus_tag="PK717_00475"
     CDS             complement(1500..4889)
                     /locus_tag="PK717_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46181.1"
                     /translation="MKKIIIFAVCLFLLAQVSPVLEVSHAKNQMVVSIHGEGQVLGNE
                     LLGRTRLGFLDPYGYPKFSPKLTKLLFDSLGVKSYDEYKDELTKHLDGLAPTFYRQDF
                     FMWAPGFEWKDAAKKIALESSKGIEHIITLNFRSFLPNNARTVGDIVEYYDGDGKDDN
                     PFGAVVKIWQIENEINMKDIFWKGTLPEYAKHLLDCYNEAKNSDPSCVVVMAGEASDA
                     ENEVYADILRELGGQPAFDAVDIHIYGQATDSLMVERCSKSFKERLLDAGYDPNTPMF
                     MTEYGTYCGKMPKMDDSSETMQAKTFAERTILALANGIKGVCYISCVDGVMDDKVDGY
                     FSQMGLIHNGITKFGNGPWHKRKVFWTYKLLSYYLSQATFDDVLEVTLGVGIRAFRFS
                     RPGLQPVYFVFLSDQETARKVPLPVTDKMRVTSLVPIGESGDEYDRLEDAIETKIIDP
                     TDLNGIYTMLAKGPIAVEPLPSRTLSEKIGVAKLSASTDIDLPVGEVYLAKENAIKWM
                     DGEKPDFSKTDLVISKLAKAGKSVILAINVEAECFSKPIEFAKFSKFIAAVVERYDGD
                     GILDAPGTLCTKFHLDARISDTSYVSESSKCYAQMIKVASRAVSMASASAKLIVGGFS
                     DNKTHIWPAILNELRKFQAEAGYPFFDIMAISNNFGSSRADSENYSFINRISRNTRKL
                     LDENGLQRTPITCLSAMNYVGAAKDYPEQTERDQANELVKRIMTMIGNGCQTACWGME
                     KDVSSNIFGSCGLLDLNGNPRLSYYSMQKLLLETSKVQVLPYDLPGGKTTVGRWIAPE
                     YNSLWGMRFGINDPFVIAWVNGSKPPTFSYVDFDFPVTQALELEYLVPDQKSGKETGG
                     KPTFKKVTVFRQDGEFKIPVDRLEPFIIRPSNATAPKIEIKIDPAIMMVNPGQKDTAT
                     ITVTGFEGEAILSAPSTKTLGTTISPEKVKSGQQATLTVSTLVASAGQQYTVAISATT
                     GQGNAEALLQIEVSNTMPVEISLWIGKKEAQVGGQKKQLAEPPTIIQGYTVVPVRFIS
                     ETFGAKVDYDQKTKEITLTFGLKPDGSYTKKVTLWVGKKEATSDFGSMLPAFQTFELP
                     VAPAIINGTTMVPLRFVSEVLGADVEWNSKERRVDIKWWPA"
     gene            4966..5514
                     /locus_tag="PK717_00480"
     CDS             4966..5514
                     /locus_tag="PK717_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR46182.1"
                     /translation="MGQSISGQLQYLSLPDVISLINQSSMNGSLQVATEDGVKGTLIF
                     VGGKLVHAVYGNIFGDEAANHIMTWTNGTFSFTPGDWDGDPTITLSVEKFILDYTLRQ
                     DEDIAEIGQSISGTHQIRLARDTSQPDGIVLEPIEWSFLSCIQDGVSIAQITSELEIT
                     TDEARRIVAKLTKLGFVKIVAE"
     gene            complement(5519..8050)
                     /locus_tag="PK717_00485"
     CDS             complement(5519..8050)
                     /locus_tag="PK717_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46183.1"
                     /translation="MKRLAAIFLVLVICFPFTSIAVEQVVIEPVGNGPTTGEIAVPVA
                     ILPLSGGDVAILDGRTSRINFFGPDGVIKNYYPIDRETIPVLPPDMSDSIPFSFAQIK
                     LISDRKSSLYYIGKDGVYQINIQTGETSKRIELEKQLLPVSIAVDGQGNIFTLDHERC
                     ITKYGSDGKEAAKIGELGKGKGKALNPYVVMALEDGSIALLDTWNEETSEQVSSDEKG
                     IMIFSGEGKFIREFGQLGLMGGDDSQIQVGFAGFSKGQEVFVLDVGFSSKPAWVIRRF
                     KTNGDFEEKIELKQSDDLVSGYVSSFFVDGNGKIALAMPFLSKAVLDANGISQELGKV
                     LEDTLKNPVSAMKLPQGKTAILEGLDRKLHIFGSDGTPQKTVALSGPKSEGPAGDSLG
                     VLSDMAMTQNELLVASGWEIRRYDNLTFEFKGSYDLQKNVDIPDIIMALASSGNKIAA
                     LDRQGQVIFMTGGLPSTFKVEGNPVDVTFDRDGNILLLFPEEKKIKAFSETGTKKFEF
                     ALEGVEMPSSICTRPSGEIMITDPAIGQIIEFSENGKNIVAYGRKGWLSQTETVQDYK
                     KEPEALAYPSRIRSNQDGTLTVLDVGNSRALNVSFKEVEPPPPEKKPAKLSVDKALLD
                     FGKMYYGKPAKTLEIAISNLGDEELEGNVTSTSKMFVVSPEKIDPETKAIKVTIDPGR
                     DDAWETFNGEIKIQTNGGSTNVPVSAQITGKKISISLLSTSFLIQTDGDEEKYDAGRA
                     GIIIKGRTLVPLRALGETLGAEVNWNASERKVTYSLDGGTVEIWINKPQAKVNGNQVN
                     IDPPAVILTSGSTYVPVRFVAEALGCDVEWVEQKKTAVVYYPKRP"
     gene            8267..9658
                     /gene="terL"
                     /locus_tag="PK717_00490"
     CDS             8267..9658
                     /gene="terL"
                     /locus_tag="PK717_00490"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HOR46184.1"
                     /translation="MPQTLSEKTFDLCKKLQFDLPAHQKKWIKCFDDPWSRRVQLAPR
                     CHGKSTIWTVIFSQAFLLLNPGARILVVSKTEQLASRLLRQIESACIRLWQAECAPKL
                     SSATATEMRLEGCDSKEPNVSCSGILGSLTGGHYDLIICDDVLDDRNTQTPQAREEVE
                     EWFYGSLLQLCLPETRLLVVGTRKHPEDLYGKLGKNPAWEFISEKAIIRWPSNFTEAL
                     EKGMPISEFFSFGDKGQLLGVKKEGKWQVLWPKMWGIDRLLLDRLMTGQAMFDREKQN
                     DVSFYLGRIFRREWLLFYDLLPERSSLSVYTACDLAISEREGSDWFAMVTVGVDSAGT
                     IYLLDCIRGHFGFSRQAEAVRDSCELHRPLLLGIEDVAYQRALIEHLSGNLAVPVRPI
                     TPLAPKQSRIRALQPLFESGKVRVAPCHELFVRELLEFPGGEHDDMIDAFEMAVSLAR
                     QGTSPRVDILEVL"
     gene            9655..10845
                     /locus_tag="PK717_00495"
     CDS             9655..10845
                     /locus_tag="PK717_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOR46185.1"
                     /translation="MKQAKLDFLSCETRQVREKPLGVSGVVEPPPYLDFEKLLELYQS
                     NEIYYRAVQVKAQFTVGQGWRLVDEGGGKEARNRLFQFLEDCNPNQTFDELLFQVMVD
                     LECLGNGYLEATRDSFGKVGRLYHVSARNIKLLRDGGFVQTIGTQKRKFASFGGGETF
                     ESDAGSPSEIVHFRKYNPSSQFYGLPDSVAAIGSTMGDILARDYNIQFFENNATPRFC
                     LVISGGSLSPKDKEELQNYMSNLKGNPHKTMVLQFPQGVTGEIKPIQTTPQEASFADY
                     RKMNRDLMVVAHGVPPHLLGIIESGNLGGGTGQSQLQNFKNLVIAPRQRFLEERINRT
                     IIREGLGISGWRFRFNEMDAEDELCRAQTDKILIDSGMISADEARARMGLPAREVEGA
                     GENR"
     gene            10855..11139
                     /locus_tag="PK717_00500"
     CDS             10855..11139
                     /locus_tag="PK717_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46186.1"
                     /translation="MSLRFSKIDRKLYFDSKLDAIWAVPAGLLLCAGWFYGLGYVVSW
                     LWNITIYRFFVPAPPLDVYTAWGAMVVIWLIFNAPFAPLRLARFFKKKPD"
     gene            11210..11638
                     /locus_tag="PK717_00505"
     CDS             11210..11638
                     /locus_tag="PK717_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HOR46187.1"
                     /translation="MAGKNGDALGTVWGWASTEALDSQGDIISTGAVKEALGAYSRWR
                     NVREMHEPRAIGVASHLSLMPKGLLVGADIYDKKCWDKIKRGIYKGFSVGGVVTSKRP
                     RNFEGKTVDVITGMTIAEISVVDQPANPEAGFILVCERKE"
     gene            11642..11917
                     /locus_tag="PK717_00510"
     CDS             11642..11917
                     /locus_tag="PK717_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46188.1"
                     /translation="MLGRFFKKGEEKKDDFECMPEQLLESLSARIKEMEERMDSISEA
                     LSSLEEKFDDAMLRLGAMEKGLLKTKTIMADDKAEPKMWASVVALLS"
     gene            11929..>12885
                     /locus_tag="PK717_00515"
     CDS             11929..>12885
                     /locus_tag="PK717_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46189.1"
                     /translation="MIGNEKLLSKAAITSGSFAQRIGLATEDVNRFIDYIVDASFLKN
                     NARVERMRGNTKKLVRLGIGEEVLRPGNYGSSGDGFSDVSTSAVTLTSKAMKAVVPIA
                     DDALMDNIEGESFADHLMRVVANQIGNQLEYAYLMGQDTGALPKNHIWEMVEGWYPRA
                     ISEGHVIDAKTVFTDRDISAEKLSRTLKTLSSKYRGKRENLRFIMSDDLYQDYVDALG
                     SRTTPLGDDAITGDSLMSFGKVTISPCALVPTNLPVPVSGGGNTTLSSASVAGDTTIE
                     VASATNVQQGDTLAIGEGATREIRKVASVVATDVTLDSALVFP"
BASE COUNT         3188 a         3026 c         3155 g         3516 t
ORIGIN      
        1 atatttattg gtctgctgtc tttttcttta attttgctcc cgtcatctag ctcaatttgc
       61 cattcgcctg aattttcaat agtttttcct atggaagcga ataccctaga tagagttgtt
      121 tttccagaat aattccaacc ataaaaaaag ttgttttttt tgaaatcatc aagtttggat
      181 ttgccccaat caaaatcttt aaatacacca aaattcttga tataagatac acgttttatt
      241 aacctcattt atataccccc atcaaacttt tataataact aaaatatttt cattaagcaa
      301 taatatggtg atctggacct aaagataccc taaaaacaaa cctaccatct atactttcca
      361 accgaagcaa tcgatcattt gtttggatta taccctctgt gtcaaattca aaaccacctc
      421 acccttaccc tctcctgaaa ggagagggaa aaaggcatgc cgcttatatt cacctatttt
      481 gggagggggg ggagaaacct tttgctcatg ccatttctga aagggaagac gcgagaattg
      541 taataaatta tgcgccttgc ttaaaaacat catctttgtg tgtcttttct tttgaatgcg
      601 gattataaat ttaccaccca cggcggggag tatgaaatat tttcttctcc ctgggggagg
      661 gttcaccgat tttggccagc tttgggaagg ttgatatttt tcctctccct ttgggagagg
      721 atgcccaaag ggcaagtgag ggggtttaag acgtctgctt tttgaaactc aaaaccacct
      781 cacccctgcc ctctcctgga aggagaggga gcaaaaacaa cattccactc tcctctcatc
      841 ttgtatctta aaaagaaaga gggaaaacaa atttgggttg caaacgtttc gccgttgctc
      901 attgttgaca gagagcgttt gcaataattc aggcaatatt tttataaatc tcagacaaaa
      961 atcaccggca accgttatcg tcttttattt gtgccataat ttctcattcc agcccagttg
     1021 ttgccaaaaa gtaagataac aggggggttg acagagcaga gggtattggc tataatgagc
     1081 ttacttttaa aatcatatta atcacaaaaa aggaggattt taattatgta tccagtgaaa
     1141 cttgatgttg attatccaga aaaattgtcc aggggcattc tgcttctcag gacattcttt
     1201 ggcgtttttt atgtcatgat tcctcacggc ttctgccttt tcttctacgg aattgccgca
     1261 atgtttgtcc aattcatcgc atggtgggca attttgttca ccggcaagta tccaaagggc
     1321 atgtttgatt tcgtggtcgg ctactacagg tggtcaatga gggtcaacgg ttacatggga
     1381 tacatgaccg acgagtatcc accattcaac ggaagaccgt aagagattat tctaccagat
     1441 tgaaatgccc cgccgttttg acggggcatt ttttattttt tgccactggc tggtgactgt
     1501 caggctggcc accacttgat gtctaccctt ctttccttgg agttccactc cacatcggcg
     1561 ccaaggactt cggagacaaa ccgcaggggc accattgttg ttccatttat gatggctggc
     1621 gcaacaggca gttcaaacgt ctggaatgcc gggagcatcg agccaaaatc agatgttgcc
     1681 tcttttttcc cgacccagag cgtcactttt ttggtgtagc ttccgtcagg cttcaggccg
     1741 aatgttaggg ttatttcctt tgttttctgg tcgtagtcca ccttggcccc gaaagtctcg
     1801 gaaatgaacc ttaccggcac cactgtgtag ccctgtatga ttgttggtgg ctctgcaagc
     1861 tgttttttct gcccgcccac ctgcgcctct tttttgccta tccacagtga aatctccact
     1921 ggcatggtgt ttgagacctc aatctggagg agggcctcgg cattgccctg gcccgttgtt
     1981 gcggaaattg caacagtgta ttgctggccg gcgcttgcaa ccagagtgga gactgttagt
     2041 gtcgcctgct ggccggattt cactttttca gggctgattg ttgtcccgag agtttttgtg
     2101 cttggagcgg agaggattgc ttcgccctca aaccctgtca cggttattgt tgccgtgtcc
     2161 ttttgcccgg ggttgaccat catgattgcc gggtcaatct ttatctcgat tttaggtgca
     2221 gtcgcattcg agggcctgat tatgaagggt tccagcctgt ccaccggtat cttgaactcg
     2281 ccgtcttgtc tgaatacggt gacttttttg aatgtgggct tgccgccggt ctcttttcca
     2341 cttttctgat ctggcaccag gtactcgagt tcgagcgcct gcgtgaccgg aaaatcaaag
     2401 tccacatagg agaaagtcgg tggttttgag ccgtttaccc aggcgatgac aaatgggtca
     2461 tttatcccaa acctcattcc ccagagactg ttgtattctg gtgcaatcca tcttccaact
     2521 gttgttttgc cgccggggag atcgtatggg agaacctgga cttttgaggt ttcaagcagg
     2581 agcttttgca ttgaatagta ggagagcctt ggatttccgt tcagatcgag cagtccgcat
     2641 gagccaaaaa tgttgcttga aacatccttt tccatacccc agcaggcggt ctggcacccg
     2701 tttccaatca tggtcatgat gcgcttcacc agctcgtttg cctggtctct ttcggtttgt
     2761 tccgggtagt cttttgccgc accaacatag ttcatggcag acaggcatgt tatcggtgtc
     2821 ctctggagac cattttcatc gagcaatttt ctggtgtttc tcgagatgcg gtttataaaa
     2881 ctgtagtttt cggagtcggc ccttgaggag ccaaagttgt ttgaaatggc cattatgtca
     2941 aagaatggat agccagcttc agcttgaaat tttcgtagtt cgttgaggat tgccggccag
     3001 atatgtgtct tgttgtcgct gaatccaccc acaatcagtt ttgcacttgc gcttgccatg
     3061 gaaacagccc tgcttgccac ttttatcatc tgcgcatagc attttgaaga ctcggaaacg
     3121 tatgaagtgt cgctgatcct cgcatcaagg tggaactttg tgcaaagggt gccgggtgca
     3181 tccagtatgc cgtccccatc gtatctttcc acgaccgcag caataaactt cgagaatttg
     3241 gcaaactcga ttggttttga gaagcattcg gcctcgacat ttattgcgag tatgacagat
     3301 ttgccggcct tcgccagttt tgaaatcacc agatcggttt ttgaaaagtc cggtttttca
     3361 ccatccatcc attttatggc gttttctttt gccaggtaca cttcacccac cggcaggtca
     3421 atatctgttg atgctgagag ttttgcaacg ccgattttct ccgagagtgt cctggatgga
     3481 aggggttcta ctgcaattgg tcctttggca agcatggtat agattccgtt caaatcggtt
     3541 gggtcaatga tttttgtttc tatggcgtcc tcaagcctgt catactcgtc accactttcg
     3601 ccaattggca caagcgaggt cactctcatt ttatcagtga ctggaagcgg tacttttctt
     3661 gccgtttcct ggtccgaaag aaaaacaaaa taaacgggct gaaggcccgg cctgctgaac
     3721 ctgaaggccc ttatgccgac gccaagtgtt acctccagca catcgtcaaa agtggcttgc
     3781 gaaagatagt acgaaaggag tttgtaggtc cagaaaacct ttcttttgtg ccatggacca
     3841 ttgccaaatt ttgtaatgcc gttgtgaatt agccccatct ggctgaaata cccatctacc
     3901 ttgtcgtcca tcacgccatc aacacagctt atgtaacata cgccctttat cccgtttgct
     3961 agagcgagta ttgtcctctc ggcaaatgtt ttcgcttgca tggtttcgct agagtcgtcc
     4021 attttcggca ttttaccgca gtaggtgccg tattcagtca taaacatcgg agtgttcgga
     4081 tcatatcctg catcaagaag cctctccttg aaactttttg agcacctttc aaccatgagc
     4141 gagtccgttg cctgtccgta aatatgtata tcgactgcat caaatgcagg ctggccaccc
     4201 agttcacgca aaatatcagc atacacctca ttttctgcat ccgaggcttc gccagccatt
     4261 acaacaacac atgaagggtc ggagttcttt gcctcgttat aacaatcaag taggtgcttt
     4321 gcgtattctg gcagggttcc cttccagaaa atgtccttca tgttgatttc attctcaatc
     4381 tgccagattt ttacgacagc accaaaggga ttgtcgtctt ttccgtcacc atcatagtat
     4441 tcgacaatgt ctccgacagt cctggcattg tttggcaaaa aactcctgaa attcagggta
     4501 atgatatgct cgatgccctt tgagctctcg agtgcgattt tctttgcagc atctttccac
     4561 tcaaaaccgg gcgcccacat gaaaaagtct tgcctgtaga aggttggagc aagtccatca
     4621 aggtgcttgg tgagctcatc cttgtattca tcataacttt tgactccaag ggaatcaaat
     4681 aacaattttg taagcttcgg ggagaatttc gggtatccat agggatcaag aaaacccaat
     4741 cttgtcctgc caaggagctc attgcccaaa acttgtcctt cgccgtgtat tgaaacaacc
     4801 atttggtttt ttgcatggga cacttccaat actggggaaa cctgggccaa caagaacagg
     4861 cagaccgcaa aaattataat tttcttcatc ctgacctcca tggtttacat tataagcata
     4921 gtattcttga ctttacagcg ctgtccaaac ataatcctaa attaaatggg ccagtcaatt
     4981 tcaggacaac tccagtattt aagcctgccc gatgtcatta gtctcataaa ccagagctca
     5041 atgaacggga gtcttcaggt ggccaccgag gacggtgtga agggcaccct catttttgtg
     5101 gggggcaagc ttgtgcatgc cgtttatggc aacatcttcg gcgatgaggc agcaaaccac
     5161 atcatgacgt ggacaaatgg cactttcagc ttcacacctg gcgactggga tggcgatcca
     5221 acaatcactc tcagtgtcga gaaattcatc cttgattaca cgctgaggca ggacgaggac
     5281 attgcagaga ttgggcaatc catttccggt acccaccaga taaggcttgc cagggacact
     5341 tcccagccag acggtatagt cctggagcca attgaatggt ctttcctttc ctgcatccag
     5401 gacggcgtat ccatcgccca gattacaagc gagcttgaaa taaccaccga tgaagcgcgc
     5461 aggatagtgg cgaagctcac aaaactcggt tttgtaaaaa tagtggccga ataggttttt
     5521 aaggcctctt cgggtagtaa accacagccg tctttttctg ctcgacccac tcaacatcgc
     5581 aaccaagcgc ctcggcaaca aaccttaccg gaacataggt tgatccactt gtgagtatta
     5641 ctgctggtgg atcgatattt acctgattac cattcacctt tgcctgtggc ttgtttatcc
     5701 agatttcgac agttccgcca tcaagggagt atgtgacttt cctttcgctg gcattccagt
     5761 ttacttcagc gcccagtgtt tcaccaagtg ccctgagcgg gacaagcgtc ctgcccttta
     5821 tgattatccc cgcccttcct gcatcgtact tttcctcgtc gccatcggtt tgtatcagga
     5881 atgatgttga aaggagcgat atcgagatct tcttaccagt tatctgcgct gacactggca
     5941 cattcgttga cccgccattt gtctggatct ttatttcacc attgaatgtc tcccaggcat
     6001 cgtcccttcc tggatcaatg gttactttta tcgcctttgt ttctgggtca attttttccg
     6061 gtgaaaccac aaacattttg gaggtgcttg ttacgttacc ttcaagttct tcgtcgccca
     6121 ggtttgagat tgcaatttcc agcgttttgg ccggttttcc atagtacatc ttgccaaagt
     6181 caagcaaagc cttgtcaaca gacagcttgg ccggcttttt ttctggtggc ggtggctcga
     6241 cttccttgaa tgaaacattc agtgcccttg aattgccaac atcaagcacg gtcagggttc
     6301 cgtcctggtt tgatcttatt cttgaaggat atgccagtgc ttctggttct tttttgtaat
     6361 cctggacagt ctcagtttgt gaaagccacc cttttctgcc gtatgctaca atattttttc
     6421 cattttctga aaactcgatg atttgcccta ttgccgggtc agttatcatt atttctccgg
     6481 atggccttgt gcagattgag gaaggcattt caaccccctc gagtgcaaac tcaaatttct
     6541 tagtgccagt ctcggagaat gcctttatct tcttttcttc agggaaaagc aggagaatgt
     6601 tgccgtccct gtcaaaagtc acgtcaacag gattgccttc caccttgaat gttgatggca
     6661 atccgcctgt catgaatatc acctggccct gtctgtcaag tgccgctatt ttgtttccgg
     6721 atgatgccag cgccattatg atgtcgggta tatcgacatt tttctggagg tcgtaggagc
     6781 ccttgaattc aaatgttaga ttgtcgtacc tgcggatttc ccatccagag gccaccaaaa
     6841 gctcgttttg ggtcattgcc atgtccgaaa gaacaccaag tgaatctcca gctggcccct
     6901 cagattttgg gccagaaaga gcaactgttt tttgcggggt gccatcagaa ccaaaaatgt
     6961 gcagctttct gtcgagcccc tcgagaatag ctgttttccc ctgtggcaat ttcattgcgg
     7021 aaacgggatt ttttagcgtg tcctcaagga cctttcccag ttcctgcgag ataccgtttg
     7081 cgtccagaac tgcttttgaa agaaagggca tggcaagcgc tattttgcca ttgccatcca
     7141 caaaaaatga tgagacatag ccacttacca gatcatcaga ctgcttgagt tctatttttt
     7201 cctcgaaatc cccgtttgtt ttaaaccttc ttatcaccca tgccggtttg cttgaaaagc
     7261 cgacatcaag gacaaaaacc tcctggccct tcgaaaaacc tgcaaaacca acctgtatct
     7321 ggctgtcatc gccacccatc aggccaagtt ggccaaattc tctaatgaat tttccctctc
     7381 ctgaaaaaat cattatccct ttttcatcgg acgagacctg ctctgaagtt tcctcattcc
     7441 aagtgtccag caatgcaatg cttccatctt caagcgccat tacaacgtat ggattaagag
     7501 ccttcccttt gccttttccc agctcaccta tttttgcagc ttcttttcca tcacttccat
     7561 actttgtgat gcacctttcg tggtcaagcg tgaaaatgtt gccttgccca tctaccgcta
     7621 tcgaaacagg aagcagttgt ttttcaagct caattctttt ggaggtttca ccagtctgga
     7681 tgtttatttg atagacaccg tccttgccaa tgtagtaaag gctgcttttc ctatccgaga
     7741 tcaatttgat ttgtgcaaat gagaatggaa tggagtccga catgtcagga ggcaacactg
     7801 ggattgtttc cctgtctatc gggtagtagt tttttatcac gccgtccggt ccaaaaaaat
     7861 tgatccgaga cgttctgccg tcgaggattg ccacgtcccc gccagaaagc ggaaggatcg
     7921 caactggaac agcaatctca cctgtggttg gcccgttgcc aaccggctcg atcactacct
     7981 gttctacggc gatggatgtg aatggaaaac agattacaag gaccaaaaaa atcgcggcaa
     8041 ggcgtttcat taattacctc ctgcacaaat tctatttctg gtatgttaca cgtcaatgcc
     8101 cggaaaaatt cacagtctca ccagaaaata attgtgagta tactataaat ataagtaaaa
     8161 atcatattag ccagctatac tctaaatgtc gatatttgac aaccagagca cctgagatta
     8221 caattcacta tcgttgggaa cccacgggag ggaggggcaa ttgggagtgc cacaaaccct
     8281 ttctgagaaa acatttgact tatgcaaaaa actgcaattt gatcttcctg cccatcaaaa
     8341 aaaatggata aagtgttttg atgacccatg gagcaggagg gtccagcttg cgccacgttg
     8401 tcatggtaaa tctactatat ggacggtgat attttcacaa gccttcctct tgttaaatcc
     8461 tggggcaaga atactggttg tttcaaagac cgagcagctt gcatcaaggc ttttgaggca
     8521 gattgaatcg gcctgcatca ggctctggca ggcagagtgc gcaccgaaac tgtccagtgc
     8581 aacagcaacc gaaatgaggc tggagggctg cgactcaaag gagccgaacg tttcctgttc
     8641 gggcatcctt gggtcgctca ccggtggcca ctatgacctg ataatctgcg acgatgtgct
     8701 tgatgaccgc aatacgcaga cgccgcaggc aagagaggaa gtcgaagagt ggttttacgg
     8761 gtcgctgctc cagctttgcc tgccagagac gaggcttctt gttgtgggaa caaggaaaca
     8821 tccggaagat ctctacggca aattggggaa aaacccagcc tgggagttta tttcagagaa
     8881 ggcaatcatc agatggccat caaatttcac cgaggcgctg gaaaaaggga tgccaatatc
     8941 cgaatttttc agttttggcg acaagggcca gctgcttggc gtgaagaagg agggcaagtg
     9001 gcaggtgctg tggcccaaaa tgtgggggat agacagattg cttctggaca ggctcatgac
     9061 aggccaggcg atgtttgaca gggaaaagca gaacgatgtg agcttctatc ttggcaggat
     9121 tttcaggagg gaatggctcc tgttctatga tctgctccct gagaggtcat cgttgtcggt
     9181 ttatacggcc tgtgatctgg ccatatccga gagggagggc tcggactggt ttgcgatggt
     9241 aacagttggc gttgattctg ctggcaccat atatcttctc gattgcatca gggggcattt
     9301 cggtttttca aggcaggcgg aggccgtgag agactcctgt gagctccaca gaccgcttct
     9361 tttggggata gaggatgtag cataccagag agcattaata gaacaccttt caggcaatct
     9421 ggcagtccct gtcagaccaa ttaccccgct tgcgcctaaa cagtcgagga taagggcact
     9481 ccagccgctg tttgagtcag ggaaagtccg cgttgcgccc tgtcacgagc tttttgtcag
     9541 ggagttgctg gagtttccgg gaggggagca tgatgacatg atagatgctt ttgaaatggc
     9601 agtttcactt gcgaggcagg gaacatcgcc gagggtcgac attctggagg ttttatgaag
     9661 caggcaaagc ttgattttct ttcatgcgag acaagacagg tcagagaaaa accgctaggt
     9721 gtatctgggg tggtggaacc acccccgtac ctggactttg agaagctgct tgagctttac
     9781 cagagcaatg aaatctacta cagggcagtg caggtaaaag cccagttcac ggttgggcag
     9841 ggctggaggc ttgtggatga gggtggtgga aaagaagcca gaaataggtt gttccagttc
     9901 cttgaggatt gtaatcccaa ccagactttt gatgagcttc tgttccaggt gatggttgac
     9961 ctggaatgcc ttgggaatgg ataccttgag gccacaaggg attcatttgg gaaagttggg
    10021 aggctgtacc atgtttctgc caggaacata aaactgctca gggacggcgg ttttgtgcaa
    10081 acgataggca cccagaagag gaaatttgcc agctttggtg gtggcgagac ttttgaaagc
    10141 gatgctggca gtccatccga gatcgtgcat ttcaggaaat acaatccttc ctcgcagttc
    10201 tacggtcttc ctgattcggt ggccgcaata ggctccacaa tgggcgatat cctggcaaga
    10261 gactacaaca tccagttttt tgagaacaat gccacgccga ggttttgcct tgtcatatcc
    10321 ggtggctcgc tctccccaaa ggacaaggag gagctccaga attacatgtc gaatcttaag
    10381 ggcaatccac acaaaacaat ggtcttgcaa ttcccacagg gtgtcaccgg ggagataaag
    10441 ccgatccaga caacaccgca ggaggcctct tttgctgact acaggaagat gaaccgtgac
    10501 ctgatggttg ttgcgcacgg tgtgccaccg cacctgcttg gcataataga gagcggcaat
    10561 cttggcggtg ggaccggcca gtcacagctg caaaatttta aaaaccttgt cattgccccg
    10621 aggcagaggt tcctggaaga aagaatcaac aggacaatca tcagggaggg actggggatt
    10681 tctgggtgga ggttccgctt caacgaaatg gatgccgaag acgagctttg cagggctcag
    10741 acagacaaaa tactcattga ttccgggatg atttcagccg atgaggccag ggcgaggatg
    10801 ggcctcccgg ccagggaggt tgaaggggct ggagaaaacc ggtaacatgt gcccatgtca
    10861 ctcagattct caaaaattga cagaaagctc tactttgact caaaactaga cgccatctgg
    10921 gcagtgccgg cgggtcttct gctttgtgct gggtggtttt atgggcttgg ctatgtcgtt
    10981 tcatggctat ggaacataac catatacagg ttttttgtgc cggcaccacc actggatgtc
    11041 tacacggcat ggggagcaat ggttgtgatc tggctcatat tcaatgcacc tttcgcgccg
    11101 ctcaggcttg cgaggttctt caaaaagaaa ccagactgat tgctgtttag tatagacaaa
    11161 aaacactggg ccctctttta gtggagggcc ttttcatttg gaggggataa tggcaggcaa
    11221 aaacggggac gctttgggga ccgtatgggg ctgggcctcc acagaagcac tggattcaca
    11281 gggggacatc ataagcacgg gggctgtaaa ggaagccctt ggcgcctatt cgaggtggag
    11341 aaatgtcagg gagatgcatg aaccaagggc aataggtgtt gcatcgcacc tctcgctcat
    11401 gccaaaaggt ctcctggttg gtgccgacat ctacgacaag aagtgctggg acaagataaa
    11461 aaggggaatc tacaagggat tttctgttgg gggtgtggtc acatcgaaaa gaccgagaaa
    11521 ctttgagggg aaaaccgttg atgtcattac cgggatgaca attgccgaga tttctgttgt
    11581 tgatcagccg gcaaacccgg aagccgggtt cattcttgtt tgcgaaagaa aggagtaagt
    11641 gatgctggga aggtttttta aaaaagggga agaaaaaaag gacgatttcg aatgcatgcc
    11701 ggagcagctc cttgaaagcc tgtccgcaag gataaaggag atggaggaga ggatggattc
    11761 gatttccgag gccttgtcat cgctcgagga gaaatttgat gatgccatgc tcaggcttgg
    11821 ggcgatggag aaagggctcc tcaagacaaa gacaattatg gccgacgaca aagccgagcc
    11881 aaaaatgtgg gcgtccgtcg tggcgctcct ttcatgacgg gagggaacat gatagggaat
    11941 gaaaaactgc tttcaaaagc tgcgataact tccgggtctt ttgcccagag gatcggcctt
    12001 gcaacagagg acgtaaaccg ttttattgac tacattgttg atgcctcgtt tctcaagaac
    12061 aatgcaaggg tcgagaggat gagggggaac acaaaaaaac ttgtgagact gggcatcggc
    12121 gaggaggttc tcaggcctgg aaattacggt tcttcaggag acggtttctc cgatgtctcg
    12181 acatcggctg tcaccctcac ctcgaaggcc atgaaggccg ttgtgccgat tgcggatgat
    12241 gcgctcatgg acaatattga aggcgaatcg tttgctgacc acctcatgag ggtggtggca
    12301 aaccagatag gcaaccagct tgaatatgcc tacctcatgg ggcaagacac cggggcactg
    12361 ccaaaaaatc acatctggga gatggttgaa gggtggtatc cgagggccat ttccgaaggc
    12421 catgtcatag atgccaagac cgttttcacc gacagggaca ttagcgcgga aaaactctcg
    12481 aggactctga agaccctgtc cagcaaatac aggggaaaaa gagaaaatct caggttcata
    12541 atgagcgacg acctgtacca ggactatgtt gatgctcttg gaagcaggac aacccctctt
    12601 ggagacgacg caataaccgg agattctctc atgagcttcg gcaaagtgac gatctcgcca
    12661 tgcgctcttg ttcctacaaa tcttccggtg cctgtttctg gcggaggaaa taccaccctg
    12721 tcatcggcct ctgttgctgg agacacaaca atagaagtcg catccgcaac aaacgtccag
    12781 cagggcgaca cactggccat tggcgaaggg gcgacccgtg aaatcaggaa agtggcctct
    12841 gttgtggcaa ctgatgtcac actggatagc gctctggtgt tcccg
//
LOCUS       DAOSRI010000009        12872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4883_length_12872_cov_4.224858, whole genome shotgun sequence.
ACCESSION   DAOSRI010000009 DAOSRI010000000
VERSION     DAOSRI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_12872_cov_4.224858"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2882
                     /locus_tag="PK717_00520"
     CDS             <1..2882
                     /locus_tag="PK717_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR46190.1"
                     /translation="ELFKPFVMEGLVRTGFTQSLRQARSLIEQQHPEVYRILSEVVKG
                     HPVLLNRAPTLHRLGIEAFDPILVEGKALQIPPLVCPPFNADFDGDQMAVHVPLTIAA
                     QMESRLLMLSSRSILSPASGQPIIKPIQDIVLGCYYISLENDTYHEKKVYDSYYEVNA
                     ALNAGLVSLHDKINFRIPKGESLFQKGKTVQVKDLKGLEIKTSVGRVIYNGRIREALE
                     KSITHRAAVAEMAGDKAPTKWNLPFFNELMAVKMIQKHISECFRLNGPAITAELLDII
                     KDIGYEMSTQAGVTIALTDMKIPPQRNEIIKKAEKEVDKITEYFDNGLISDNERYQAI
                     VTLWTGATDDIKGAVFANFGKFDSVHMMANSGARGSPKQINQMVGMRGLMTDPTNKII
                     EFPIKSNLREGLTVLEYFMSTHGSRKGLADTALRTSDSGYLTRRLVDVSHDIIVREYD
                     CSQTEIYPIIIANKVVKPISKQAVGKIAIEDIVNPETGEVIISAGQTITWKKAQLMDE
                     ANIKIAKVKTSIDGVVYTALYDDKTIVEQLGDRIIGRIAIEDILGYPSKTVPVDDESV
                     VGGILAHDIVKRGKIHYERGSKITKQMLKTAKNLDFEKIAVATGEPWAIVKAGQMVDE
                     KAVEEINDAGIAQVRMRSPLTCQSTEGVCALCYGRDLSSGNLVNVGEAVGIVAAQSIG
                     EPGTQLTLQTFHTGGVASADITTGLPRVEELFEARKPKGEAPVTEISGKITIDQGKDM
                     LNVIVTSKDGAQKTYEVPYTSQLLVQNNEEVEAGTPITEGSLNPHELLRIKGVMATQS
                     YLIEAIQKVYKSQGVDINDKHVEIIIRQLLRRIKIKDSGDSRFLPGETADKQEIEEEN
                     RYLIAQNKKPATFEPILQRVTKSAITSESFLSAASFQETPRVLAEAAVKGKIDPIHGL
                     KEAVIVGQLIPAGTGTPCYLDMVVETKEETPFTTDQETAPPKQQD"
     gene            3022..7140
                     /locus_tag="PK717_00525"
     CDS             3022..7140
                     /locus_tag="PK717_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46191.1"
                     /translation="MKRTIFLAIITIFLITTLTTAIAIDDCDWNFFHGTAESQGYSSC
                     GPGSAELRELWTFTANGRIDSPAVISNGKVFFCDHARSTPNASLHCIDLTTGKEMWKA
                     RMSGYGNYCAPAVYGGKVYSGCFAGYKPSEELFRGCKFYCFNALDGKELWKFDTKCPV
                     MCSPVVVGSSVFFGTFDENNFIYCLDATSGAMKWKTKIGAMIYSSSPVCSNGKLYIGA
                     SDGTIYCLNQENGAIVWKKTYGQSDNDFIASSAHKDAKIYFGNSKGQLYCVDANTGNE
                     VWMIKLDHPIASTPGISGNMLVIGTMDPEQQNIKDGSSDCSIYGIDIASGKIIWKKFA
                     DIGSTTDPAISNGIVYLTFGKGIAYALDLETGATLWQASLDSYAGSSFSISNNMVVVG
                     EADGTLHCYTESIVNQHIIDFGEITSGQKLEREINVRNNQQSQQEFKVTITNGDWIRI
                     DKTKISLNGRQDGKIKVSLIPDKMAQSGIYKGKITISSGSLEASVELMAYTLSGSEED
                     PNSCEWKNFGKTSTHSFTCDPKCAPTSNYLKKAWITALKAGESNYSLLSDQSKIFVAL
                     ANSNHYGKLFCLKRSSGEKLWNESMESTNEGSASIEGGNILFGSSYELYSMSVETGKE
                     RWSFKIDADKNSKVNIKPISRPGRIFFTASDGNLYSVASDTGEKIWSTPLGESVSAYP
                     AYDKGKVYAVSASKLYCIESSTGLIIWESSLSGKPTNSPSISNGNIFIVTSIASTNNI
                     GASEQTFVNCIDLVSGKNVWSSKIEGKIHSSIISDGEFVYFGCSDGFMYCLDAKKGTR
                     KWIFNSEGIISATPVLSGDKLFFGNTALKIFCIDKNAGSLVWTVPTKAPITSGVILVN
                     GDLYAVDDEGIVYCLNNDENAVPTKLEIRNIGEKVELEQTVQLEGAVLDAKDNQMRGY
                     PVSWASEPEGIVTISDKGVLKATSIGKCKIICRTEKLEANLDIEVVGKIPPECSDEID
                     FGTVEPGVEKTLELKVKNQSCTSIDIRISESDEWFECSLPSVHIQKGETYTLKIRTVP
                     DKLPDGAWLTGKLNLDRGDDKKDIKVKVVGKGISFSRTEIDIGDIQVSQIQSRTIIIS
                     NNTKNDVQMAISSSANWIKYSPESVAIKSGKDGSVTITIETWEFEGNKTYNGLVMFDW
                     PLGNVEVPVKVNALPDKTPPQIRISDTITIYNATEAEITGETEKGCSVIVDGIQAKLT
                     GTQFSAKIKVVQAPSKKTIIIESTDKSGNKTVKEVLIVNIKRRKVVMTVGVATMTVNG
                     KSVAINPPPTILRGATMVPVRAIADAFDATTEFNKDTNSVTITLDGRKIIIFIGSQTA
                     IVDGETKLIKPPAIIYNGKTMVPFRFIAEALGAQVEWDPKTKTITLNMDKMP"
     gene            7152..9920
                     /locus_tag="PK717_00530"
     CDS             7152..9920
                     /locus_tag="PK717_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46192.1"
                     /translation="MKKTIAILISLVVFCSITPPMITAEDDDDNNSCIYPMFGGDEEH
                     SGVAQDHCIEDVSLLKKKWESKIPESDFIGIESLVTAKGKIYTITEDLDFKKTMMHCL
                     RSTDGKIIWSRQLDLPMLFSQMAISGEKIFYGDAMGMKCLSTKSGMPLWSYDVDISEV
                     MQTIIYSPKPTIYDDKIYTTFGLGKIACFSMSGGKLWLDKITSSSNYYSYSSPIISNN
                     KLFVLMDNLYCLSPSNGRQIWSSFVGNGGTGLANISSYKDKIIASIDQRSSNTNYFAN
                     DDEDKPTPNTIVCVEQEKGKVLWKFKTQDDVFSGFAVDQDSNSVFFGCVDSKMYGLDI
                     ETGKEKWNLTLPEKIIGSPTIFGKYILIGCYDDNLYCINKLSGKIAAKYETNGAVPTP
                     PAVLDNMVIVSTTDNNIICFEGKSGPKPTSIDISPKNPTAFVNEKIQFEATVLDQDGK
                     KIIGLQPVWKVDPPKYGTIDQFGVFLPAEKGNCTITASIGKLSASTNVEVYMKQPLEF
                     TEILDFGEVEQDTTRTIEFVIKNPEPYEAKFKITTKSKWVTFDPSSGKIDPENTITIK
                     ATANPESLEMDQLVTEKAELEYNYGEGEITIQAQKTYNPPPLLAPKEIELAGVKQGEN
                     IKKSFQIINQAKKEYKISCKSTTPWLKVPVEEIIIPADNEATFEFEISTNGLALSKTH
                     TGIVIVKWPKGELELPVKVEMVKDVTSPKITIDKIGDLVNQDSYVIMGSANETVTAKA
                     IFGGIEINGKQNAPNTFTITVPLKPAPSKNIFKIEVTDESGNKTMADVIIINIAEKRV
                     ELTVGSQTMMVDGVEVQINPPPYIKGGTTMVPLRAIADAFGAEVNYDGKTKSISIKYG
                     QIKITMVVNKTEAIVNGKRVKISPPPETHKSGRVTVPFRFIAEAFGAEVTYDATTKSI
                     ILTKQIRP"
     gene            complement(10209..11159)
                     /locus_tag="PK717_00535"
     CDS             complement(10209..11159)
                     /locus_tag="PK717_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR46193.1"
                     /translation="MKNQNNRIIATEDGQKKRAGKKTPFFSGPIWHEIVRYSELVIGA
                     VIFGFGQVTFLVPAQLAPGGLNGFGIIINSATGFPVGLAYFLMNIPGFLWAFKLLGPR
                     YLLRTGVAIAVSSATMDLCAMFIVPKLNIFPNGPDYVLASIFGGVLLGIGVGLIFRSG
                     GSAGGTEVISQLLYWSTGYEYGKAVLVLDSVIIAIVTLYFRNIQLALYSIITLFIYSM
                     VLNMVASGMTSTKLVIIITNECDKIRNVIMEKLGRGVTILDAEGGYRFDPKKMVICAV
                     PQSQVSRIKEFVREVDQESFMMVSDLSEVVGKGFERKLPK"
     gene            complement(11143..>12872)
                     /locus_tag="PK717_00540"
     CDS             complement(11143..>12872)
                     /locus_tag="PK717_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR46194.1"
                     /translation="GDFSSTGPYLGPRGLKFKPEVSAPGMWIFSTVYDEKNPKDAWDS
                     YSGTSMAAPFVTGMSALVKSYHPDWLPWQIKASLMNTAKFQINPLNGDPYPLVIQGSG
                     LANVYDACTTPVITDPPAFTFHGGNYKDGVKINLINVDSKRATVKLTSKVTFVGDVEG
                     VDISITQSDEVVSPPPQSSPRDATASLDLKINLDKQKVPKNRVVEGSIEITIDSSTQI
                     HVPFMALFGEIEIYNSLKSFSNLLVQNDKIDVAQGEKPGTVKFHVGVGSKSFFWGNQY
                     DNFAYMTRISVLDKILDRWRDIYEGNYLIPGDYEFEWDGKDIDGDLFLPNGPNGIGSF
                     MYYEAIVGSTQNKLVESSVPRDAQAWVEVIGSDKLPLPELNLSVNPEFGALNKTLTVK
                     MSLARSSQNKVIRFSLLYDAKMVTYTGNYRRGSMTEDSTSMMISIEDNQDGKMTVEIS
                     PSYAGLTLDGTGDLMEIDFTCNRVGGTEFDTSFVEILDNEIKNIRFVRPLPVEVLITD
                     RYLLIGDFNVDWVVDDKDLVIFGQQFGKKEGDPDYNKLCDINRDKVIDEKDFIYFSKH
                     FGERSEKPK"
BASE COUNT         4316 a         2647 c         2599 g         3310 t
ORIGIN      
        1 tcgaactctt caagccattc gtgatggaag gtcttgtcag aactggcttc acccagagtc
       61 tcagacaggc aaggtctcta atcgagcagc aacatccaga ggtttacaga atcctatcag
      121 aggtcgtcaa aggtcacccg gtgcttctaa atcgcgctcc aacacttcac aggctgggca
      181 ttgaagcatt tgacccaatc cttgttgaag gcaaagcact ccagatacca cctctcgtct
      241 gcccgccatt caacgctgac ttcgacggtg accagatggc agttcatgtt cctctgacca
      301 ttgcggcaca gatggaatca agattgttga tgctttcatc gcgctcgatt ctttcgccag
      361 cctctggaca gccgatcatc aaaccaatcc aagacatcgt tttaggttgc tactacatct
      421 ccctggaaaa tgatacctat catgagaaga aagtttatga cagctattat gaggtaaatg
      481 ctgcactgaa tgctggcctt gtaagccttc atgacaaaat caacttcagg attccaaagg
      541 gtgaaagcct cttccaaaag ggtaaaaccg tccaagtcaa agacctcaag ggccttgaga
      601 taaaaacatc agttggaaga gtcatctaca atggcaggat cagagaagcc cttgaaaaga
      661 gcattaccca tagggcagcc gttgctgaga tggctggcga caaagcccca acaaaatgga
      721 accttccatt cttcaatgaa ctgatggcag tcaagatgat ccagaagcac atttcagagt
      781 gtttcagact caatggacct gcaataaccg cagaattgct cgacatcatc aaggacatcg
      841 gatatgaaat gtcgacccag gccggtgtaa caatcgccct gacagatatg aaaatcccac
      901 cacaaagaaa tgaaatcatc aagaaagcag aaaaagaagt agataaaata acagaatatt
      961 ttgacaacgg tcttatttct gacaacgaaa gatatcaagc aatagtaaca ctctggactg
     1021 gtgcaactga tgacatcaag ggcgctgttt ttgccaactt cggcaagttt gattcggtcc
     1081 acatgatggc aaactcggga gcaagaggat cacctaagca gatcaatcag atggtcggaa
     1141 tgaggggact tatgaccgac cctaccaata aaatcatcga gtttccaatt aaatccaacc
     1201 tcagagaagg ccttacggtt cttgaatact tcatgtcaac ccacggttcc agaaaaggtc
     1261 tcgcagacac cgctctaagg acatcagact ctggttatct tacaaggcgt cttgttgatg
     1321 tttcgcacga catcatagtc agggaatatg attgctccca gacagaaatt tatccaataa
     1381 tcatcgccaa caaggttgta aaacccattt caaaacaggc tgtcgggaaa attgccatag
     1441 aagatatcgt caatcctgaa actggtgaag taatcatcag tgcggggcaa acaatcacat
     1501 ggaaaaaagc ccagctgatg gacgaagcga acatcaagat tgcaaaggtc aaaacatcca
     1561 ttgatggcgt tgtttacaca gccctctatg acgacaaaac aatcgttgaa cagcttggcg
     1621 acagaataat aggcagaata gcaatcgaag atatccttgg ttatccttca aaaacagtgc
     1681 cagttgatga tgaatcagtt gttggcggga ttttagccca tgacatcgtc aaacgtggta
     1741 agattcatta tgaacgtggg tccaaaataa ccaagcaaat gcttaaaaca gcaaagaatc
     1801 ttgatttcga aaagattgct gttgcaactg gcgagccatg ggcaatcgtc aaggcaggcc
     1861 agatggttga tgaaaaggca gtcgaagaga tcaatgatgc tggaatagca caagtcagaa
     1921 tgcgttcacc acttacttgc cagagcaccg aaggtgtatg cgctctttgc tacggcagag
     1981 acttatcctc cggaaacctt gtgaatgttg gcgaagcagt tggaatcgtc gccgcccaat
     2041 caatcggaga accaggcaca cagctcacac tccagacatt ccacaccgga ggcgtcgctt
     2101 cggccgacat caccaccggt cttccaaggg tcgaggagct ttttgaagca aggaagccaa
     2161 agggagaagc tcctgtcact gaaatatccg gcaagataac aatcgatcag ggcaaggata
     2221 tgctcaatgt tattgtaacc tccaaagacg gtgcccaaaa aacatacgaa gtgccataca
     2281 caagccagct tcttgttcaa aacaatgagg aagtagaggc aggaacgcca ataaccgaag
     2341 gctcactcaa tccacacgaa ctattgagaa ttaaaggtgt aatggccacc cagtcttatc
     2401 tgatcgaagc aattcaaaaa gtctataaat cacagggtgt agacataaac gataagcatg
     2461 tcgaaataat catcagacag ctcctccgca gaataaaaat caaagattcg ggagattcaa
     2521 gattcttgcc tggtgaaact gccgacaagc aggaaattga ggaagaaaac agatatctga
     2581 ttgcacaaaa caaaaaacct gcaacattcg aaccaatact tcaaagggtc accaaatcag
     2641 ctatcacatc cgaatcattc ctctctgcag cttcgttcca ggaaacacca agggttcttg
     2701 ctgaagcagc tgtaaaaggc aagatagacc cgatacacgg cctcaaagaa gcagtcattg
     2761 taggacaact catacctgca ggcacaggaa ccccatgcta tcttgacatg gttgtggaaa
     2821 caaaggagga aactcctttc acaacggatc aggaaactgc accacccaaa cagcaggatt
     2881 aactttcgta aacaaaaatt tacaaaaagg cgaccctata gggtcgcctt ttttatttta
     2941 cggcctataa tggttcggac tgggttgttg aaatttaaat tttattattt acaataactt
     3001 gttgccatag gagggacaaa tatgaagagg acgatatttt tggcaataat aactattttt
     3061 cttataacaa cactgaccac cgctattgca attgatgatt gcgactggaa tttctttcat
     3121 ggtacagctg aatcacaagg ctattcatca tgtggcccag gctctgcgga acttagagag
     3181 ctttggactt ttactgcgaa tggaagaata gattctccag ctgtcatatc taatggaaag
     3241 gtattttttt gtgatcatgc gagaagcact ccaaatgcca gtttgcactg catcgatctt
     3301 acaactggaa aagaaatgtg gaaagcaagg atgagtggtt atggaaacta ttgtgctcct
     3361 gctgtctatg gcgggaaagt ttactccggc tgtttcgcag gttataaacc atctgaagag
     3421 ttgtttcgtg gatgcaaatt ttattgcttc aatgcactcg atggcaaaga gctctggaaa
     3481 tttgatacaa aatgccctgt catgtgttca ccagttgtgg ttggatcgag tgtttttttc
     3541 ggtacatttg atgaaaacaa tttcatttac tgccttgatg ccacatcagg ggcaatgaaa
     3601 tggaagacaa aaataggagc tatgatttat tcatcttcac cggtctgctc caatgggaaa
     3661 ttatatatag gcgcatctga tggaacaatt tattgtttaa accaggaaaa tggagctatc
     3721 gtctggaaaa aaacatacgg ccaatcagat aatgatttta ttgcaagctc ggcccacaaa
     3781 gatgcgaaga tttattttgg aaattctaaa ggacagttgt attgtgttga tgcaaatacc
     3841 ggaaatgaag tctggatgat caaacttgat catccaatcg cgagcactcc gggaatatca
     3901 ggaaatatgt tggtcattgg cacaatggac ccagaacagc agaacataaa ggatggatca
     3961 tcggattgca gcatatatgg catcgacatc gcatcaggga aaataatatg gaaaaaattt
     4021 gctgatattg gtagcacaac agaccctgct atctcaaatg gaattgtgta tctgactttt
     4081 ggcaaaggaa tagcatacgc acttgatctc gaaacaggtg caacactatg gcaagctagt
     4141 ttggattcat acgcgggtag ttctttctca atttccaata atatggtagt tgttggagaa
     4201 gccgatggaa ctttgcactg ttatactgaa tcaatcgtca atcagcacat tattgatttt
     4261 ggcgaaatca catctggcca aaagctggaa agagaaatta atgtcagaaa caaccaacaa
     4321 tcccaacaag aattcaaagt caccataaca aatggtgatt ggataaggat agataaaaca
     4381 aagatatcgc ttaacgggcg tcaggatggg aaaatcaaag tttccctgat tcctgataaa
     4441 atggcccaaa gtggcattta taaaggtaaa atcacaataa gttctggatc tttggaagcc
     4501 agcgttgaat tgatggcata cacactgtca ggatcagaag aggatccaaa ttcatgtgaa
     4561 tggaagaatt ttggcaaaac atccactcac agcttcactt gcgaccccaa atgtgcaccg
     4621 acatcaaatt acttgaaaaa agcctggata acagcattaa aagctggtga atcaaactat
     4681 tcgctacttt ctgaccagtc aaaaattttt gtcgcactcg ctaattcgaa tcactatgga
     4741 aaattgttct gtttgaaaag atcaagcggt gaaaaactct ggaatgaatc catggaatca
     4801 acaaatgagg gttcggcctc tattgaaggt ggcaatatcc tctttggttc cagctacgaa
     4861 ctatattcga tgagtgtgga aaccggaaaa gaacgatgga gcttcaaaat agacgcagat
     4921 aaaaatagta aagtaaatat taaacccata agtagaccgg gcaggatatt cttcacagcc
     4981 agcgatggga atctatatag tgttgcctcc gatacaggag aaaaaatctg gtctactcca
     5041 ctaggagaaa gtgtatccgc atatccagca tatgacaaag ggaaagtgta cgctgtatca
     5101 gccagcaagt tatattgcat tgaatccagc acaggattaa taatctggga atcatccctt
     5161 agtggaaaac caacaaattc tccatcaata agtaatggca atatttttat tgttacctct
     5221 attgcatcta ctaacaacat aggtgcatca gaacaaacat tcgttaattg cattgatcta
     5281 gtatctggga aaaatgtatg gtcctcaaaa attgagggga aaattcactc aagtatcatc
     5341 tcagacggtg aatttgtgta ttttgggtgc agtgatggtt tcatgtactg ccttgatgca
     5401 aaaaaaggta caaggaaatg gatttttaac tccgagggca taatatcagc aacgccagtt
     5461 ttaagcggcg acaaactctt ttttggcaac acagctttaa aaatattctg catcgacaaa
     5521 aacgctggtt ccttggtatg gacagttcca acaaaagcgc ccatcacaag tggtgtaatc
     5581 cttgtaaatg gagaccttta tgcagtcgac gacgaaggaa tcgtttattg tttaaataac
     5641 gatgaaaatg cagttccaac aaaacttgaa atcaggaata ttggagaaaa agtagagctg
     5701 gaacaaacag ttcagcttga aggcgcagta ctggatgcaa aagataatca gatgagaggt
     5761 tatccagtat cctgggcgtc agaaccagaa gggattgtta caataagcga taagggtgtc
     5821 ctcaaagcaa caagtatcgg caaatgcaaa ataatatgcc gcacagaaaa acttgaagca
     5881 aatcttgaca tagaagttgt agggaaaata ccacctgaat gttcggacga aattgacttt
     5941 ggtacggtcg agcctggtgt agaaaaaacc ctggagctaa aggtaaaaaa ccagtcttgc
     6001 acctcaattg atattagaat tagtgagagc gacgagtggt ttgaatgctc gctgccaagt
     6061 gttcatatcc agaaaggtga aacatacact ctaaaaatca ggactgtgcc agataaactg
     6121 cctgatggcg cctggttaac aggaaaactg aatcttgata ggggagatga caaaaaagac
     6181 atcaaggtga aagtggtagg caagggaata tcattctcaa gaacagaaat tgatattggg
     6241 gacatccaag tttcgcaaat acagtctaga acgatcataa tatcaaacaa cacaaaaaac
     6301 gatgttcaaa tggccatttc ttccagtgct aactggatca aatacagccc tgaaagtgta
     6361 gcaatcaaat ctggcaagga tgggtcggtt acaataacta tcgaaacttg ggaatttgaa
     6421 gggaacaaaa catacaatgg actagtcatg tttgattggc cccttggaaa tgtcgaggta
     6481 cccgtaaaag tcaatgccct gccagacaaa acgcctccac aaatcagaat ttccgatacc
     6541 ataacgatat ataacgcaac agaagcagaa attaccggag aaacagaaaa aggctgctct
     6601 gtgattgtgg atgggattca ggcaaaactc actggaaccc agttcagcgc aaaaataaaa
     6661 gttgtccaag caccttctaa aaaaacaata atcattgaaa gtactgacaa atcaggcaat
     6721 aaaacagtca aagaagtgct tattgtaaac ataaaaagaa gaaaggttgt catgactgtc
     6781 ggagtggcaa ctatgaccgt aaatgggaaa tcagtggcca taaacccacc accaactata
     6841 cttcgtgggg caacaatggt acctgtaaga gcaatcgccg atgcttttga cgctacaaca
     6901 gaatttaaca aagataccaa ctctgtcaca ataacacttg atggaagaaa gataatcata
     6961 tttattggct cccagactgc aatcgttgat ggagagacca aattgataaa accaccagca
     7021 attatctaca acgggaagac aatggtcccg tttagattta ttgctgaagc gcttggcgcg
     7081 caggtggaat gggacccaaa aacaaaaaca ataacactta acatggacaa aatgccatga
     7141 gggagggcag aatgaaaaaa actatcgcta ttttgatttc actggtggtc ttttgctcaa
     7201 tcacaccgcc gatgataacc gcagaagatg atgatgacaa taacagctgc atttatccaa
     7261 tgtttggtgg agatgaagaa cattcaggtg ttgctcaaga tcattgcatt gaagatgttt
     7321 cacttctgaa gaaaaaatgg gaatccaaga taccagaatc agattttatt ggtatagaat
     7381 cactagtgac tgctaaagga aaaatctaca ccattacaga agatctcgat ttcaaaaaaa
     7441 caatgatgca ttgcctgaga tcaactgatg gcaaaattat atggtcaaga caactggatc
     7501 ttccaatgtt gttttctcaa atggcaattt caggcgagaa aatcttttat ggcgatgcta
     7561 tgggcatgaa atgtctttca acaaaaagcg gcatgccctt atggagctat gatgtcgaca
     7621 tctcagaggt aatgcaaaca atcatttact ccccgaaacc aacaatttac gacgacaaga
     7681 tatacacaac ttttggcctt ggaaaaatag catgtttttc catgtcagga ggaaaactct
     7741 ggcttgataa aattacaagc agttcaaact attattccta ttcatcccca atcatttcta
     7801 acaataaatt atttgtgcta atggacaatc tctattgcct gtccccatca aatggtcgcc
     7861 aaatatggag ttcatttgtt ggcaatggtg gaacaggttt ggcaaacatt tcttcataca
     7921 aagataaaat tatcgcgtca atagatcaga gaagttccaa tacaaactat tttgcaaatg
     7981 atgatgaaga taaaccaaca ccaaatacaa tagtttgtgt cgagcaggaa aaagggaaag
     8041 ttctctggaa gttcaaaacc caagatgatg tcttttctgg ctttgcagtt gatcaagatt
     8101 caaattcagt tttttttggt tgcgtagact caaaaatgta cgggcttgac atagaaacag
     8161 gaaaagaaaa atggaacctt acactaccag aaaaaataat tggttcccct acaatttttg
     8221 gaaaatacat tcttattggg tgctatgatg acaatcttta ctgtatcaac aagctttctg
     8281 gcaaaatagc tgcaaaatat gagaccaatg gagctgttcc aacaccacct gctgtgcttg
     8341 ataacatggt cattgtttca actacagaca acaacataat ctgtttcgag gggaaatctg
     8401 gtccaaaacc aacttcaatt gatatttctc caaaaaatcc aacagctttt gtaaatgaaa
     8461 agatacaatt tgaagcaaca gttcttgacc aggatggcaa aaaaatcatt ggtttgcaac
     8521 cggtttggaa agttgatcct ccaaaatatg gcacaatcga ccaatttgga gttttccttc
     8581 cagctgaaaa ggggaattgc actattacag cctcaattgg taaactctct gcatctacaa
     8641 atgttgaagt ttatatgaaa cagccactgg aatttacaga aatattggat tttggcgaag
     8701 ttgaacaaga cacaacgcgt acaatcgaat tcgtaattaa aaaccccgaa ccatacgaag
     8761 caaaattcaa aataactaca aaaagcaagt gggttacatt tgatccttca agtgggaaaa
     8821 ttgacccgga aaacacaata actatcaaag cgacagcaaa tcctgaatcg cttgagatgg
     8881 accaacttgt aaccgaaaaa gcagagcttg aatataatta tggtgaaggc gaaatcacta
     8941 tccaggctca aaaaacatat aatccaccac cacttcttgc gccaaaggaa attgaactgg
     9001 ctggtgtcaa gcagggtgag aacatcaaaa aatctttcca gatcatcaac caggcaaaaa
     9061 aggaatataa gataagctgc aagtccacca cgccatggtt gaaagttcca gtagaagaaa
     9121 taattattcc ggcagacaac gaagccactt ttgaatttga gatatcaacg aatggcctcg
     9181 cactaagtaa aacacacact ggcatcgtaa tagtcaaatg gccaaagggt gaactagagc
     9241 tgcctgtaaa agttgaaatg gtgaaagatg ttacatcacc caaaataaca atcgataaga
     9301 taggggacct agtaaatcag gattcctatg taattatggg tagtgccaat gaaactgtaa
     9361 ccgctaaagc tatttttggt ggcatcgaaa taaatggaaa acaaaatgcc ccaaatacat
     9421 ttactattac tgtcccgcta aaaccggccc caagcaaaaa tatattcaag attgaagtaa
     9481 ccgacgaatc aggcaacaaa acaatggctg atgttataat tataaacatt gccgaaaaga
     9541 gagtggaact cactgttggc agtcaaacaa tgatggttga tggagttgag gtacaaatta
     9601 atcctccacc ttatataaaa ggtgggacaa caatggttcc actgagggca attgcagatg
     9661 catttggggc cgaggtcaac tatgatggga aaaccaagag tatctcgata aaatatggtc
     9721 agataaaaat aactatggtt gtgaataaaa cagaggctat cgtaaacgga aaacgtgtaa
     9781 aaatatcacc tcctcctgaa acacataaat ccggaagagt aacagtgcca ttcagattta
     9841 tagctgaggc attcggggct gaggtcactt acgatgccac aacaaaatca ataatcctca
     9901 caaaacaaat tcggccataa gatcaaaacc tactttaaaa gcccgaggat atcctcgggc
     9961 ttttttattg atttttacat tattatcttg gtatatatga ttgcattaaa ggataattta
    10021 atttagtaat atgatagttt gagttcaaag atttataaca aaaaaatggg tagatttttt
    10081 tggggggtat tcctattcct ctccctgttg gtttcttaaa aattcaaaaa taataggtta
    10141 ttttagatga tcaaaagaaa ataagtatag ataattattt cttgtgaatc attttagaaa
    10201 tgatatattt acttgggtaa ttttctctca aaaccttttc caacaacttc tgaaaggtca
    10261 gacaccatca taaaagattc ctgatcaacc tccctaacaa actcttttat cctgcttacc
    10321 tgagattgag gaactgcaca aataaccatt ttcttgggat caaagcgata gccgccttca
    10381 gcatccaata tcgtaacgcc tcttccaagt ttctccataa ttacatttct tatcttatcg
    10441 cactcgttgg tgatgattat cacaagtttt gttgacgtca tcccagacgc aaccatattc
    10501 aaaaccattg aatagataaa aagggtgatg attgaataaa gggccagttg aatatttcta
    10561 aaatacaatg tcacaatagc tataataacc gaatcaagaa ctaggacagc ttttccatat
    10621 tcatagccag tcgaccagta taaaagctgg gaaatgacct cggttcctcc ggcacttcct
    10681 ccgcttctga aaatcaggcc gacacctatc ccaaggagaa ctcctccaaa tatggatgcc
    10741 aaaacatagt ccggcccatt tggaaagata ttgagttttg ggactataaa catggcacat
    10801 aaatccatgg tagctgatga aacagcaatg gcaacgccgg tccttaagag gtaccttggc
    10861 ccaagaagtt tgaacgccca tagaaacccc gggatgttca tgagaaaata cgcaagacct
    10921 acgggaaaac cggtggcact attgatgata atgccaaaac cattcaaccc acctggagcc
    10981 aattgtgctg gaacaagaaa agttacctga ccaaagccaa atatcacagc ccctataaca
    11041 agttctgaat accttacgat ttcatgccag ataggaccag aaaaaaaggg cgttttctta
    11101 cccgcccttt ttttctgacc atcttctgta gcaataatcc tattattttg gtttttcact
    11161 tctttctcca aaatgtttag agaagtagat aaaatccttt tcgtcgatga ccttgtccct
    11221 gttaatgtcg cacagcttgt tgtagtcggg atctccctct ttcttaccaa actgctgacc
    11281 gaaaataaca agatctttgt catctaccac ccagtctacg ttgaaatcac cgatcaaaag
    11341 ataccgatcg gttataagca cctcaactgg caacggtctt acgaatctaa tatttttaat
    11401 ttcattgtca agtatttcta caaaagaagt atcgaactcc gttccaccaa ccctattgca
    11461 agtaaaatca atctccatga gatcgcctgt tccatccaat gttaaaccag cataagatgg
    11521 cgagatctcc acggtcattt taccgtcctg gttatcctcg attgaaatca tcatacttgt
    11581 tgaatcttca gtcatagaac ctcttctata attgccagtg taagttacca tttttgcatc
    11641 atagagcaga gagaacctta tgaccttatt ttggctcgac cttgcaagag acatcttcac
    11701 tgtgagtgtt ttattcaatg caccaaactc tgggttaaca ctaagattca gctcaggaag
    11761 cggtaatttg tctgaaccaa tcacctccac ccaagcttga gcatcccttg gcactgaact
    11821 ctcaacaagt ttattttgcg tactaccaac tatggcttca tagtacataa aacttcctat
    11881 gccatttggc ccatttggca agaacaaatc tccatcaata tcttttccgt cccattcgaa
    11941 ttcataatca cctggaatga gataattgcc ttcataaata tctctccacc tatccaagat
    12001 cttatctaga acagatatcc ttgtcatata ggcaaaattg tcatattggt tgccccagaa
    12061 gaacgatttt gagccaactc caacatgaaa cttgactgtt ccaggttttt caccctgggc
    12121 aacatcaatc ttgtcattct gcacgagaag atttgaaaac gatttaagag agttgtaaat
    12181 ctctatctcg ccaaacaaag ccatgaacgg aacatggatc tgggttgaac tatcgatggt
    12241 gatttcaatt gagccctcaa caactctatt ttttggaact ttttgcttgt ccagatttat
    12301 tttgagatca agcgaagcag ttgcatccct tggtgaggat tgtggcggtg gagagaccac
    12361 ttcatctgat tgagtaattg atatatcaac accttcaaca tccccgacaa aggtcacttt
    12421 ggaagtgagt tttactgtag ccctcttcga atcaacattg ataagattta tctttactcc
    12481 atccttgtag ttaccaccat ggaaggtaaa tgctggaggg tccgttatta ctggcgttgt
    12541 acaagcatca taaacattgg caagccctga cccctggatt accaatggat aggggtcacc
    12601 attgagtgga ttgatctgga acttggctgt attcataagt gaggctttta tttgccatgg
    12661 tagccaatca gggtgataac tctttacaag tgctgacatg ccagttacga atggtgccgc
    12721 cattgatgtt ccagaatatg agtcccaagc atccttcgga ttcttctcat cataaaccgt
    12781 tgaaaaaatc cacattccag gcgcagaaac ttctggtttg aatttcagac cccttggtcc
    12841 aaggtacgga ccagtcgagg aaaaatctcc ag
//
LOCUS       DAOSRI010000010        12810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_4915_length_12810_cov_4.186358, whole genome shotgun sequence.
ACCESSION   DAOSRI010000010 DAOSRI010000000
VERSION     DAOSRI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_12810_cov_4.186358"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK717_00545"
     CDS             complement(<1..461)
                     /locus_tag="PK717_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR46195.1"
                     /translation="MLDVMSVTKSYPGKMALSNVSFELKKGEFAFLMGSSGSGKSTLL
                     KLIYAAEQPTSGRIFLENKDVSKLSPYRTALLRRRIGIVFQDFKLISTKTVWENVAFA
                     MEVQGAASRNIKHKVAEVLGFVGLNLRSRDFPNMLSGGEQQRVAIARAIAKR"
     gene            complement(462..1346)
                     /locus_tag="PK717_00550"
     CDS             complement(462..1346)
                     /locus_tag="PK717_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR46196.1"
                     /translation="MSYNFKREIKRYAGIAAGVIIESLSYALFLVPNKIAPGGMTAVA
                     IIVNSLTNGFLPVGFSYFLLNLPWLGLAWKKMGHKYITRTLVISFISFVFTDLFIFLI
                     NKYLPTKLTSDMLLASVFGGILNGLGIGLTFKNRASAGGTDLISQVIHYYTKFPFGQT
                     VLIVDSVVIIGLAFYFQNANLFIYAMVALVISSFVIDAVQEGISFTKCMSIITTETEQ
                     IRENLILHLGRGFTIIDVKGGFTGDDKKMLVMAVHRSQLAEAKKIILETDPKSFIMIG
                     PLNEVLGEGFKNPKMEEW"
     gene            1589..1876
                     /gene="groES"
                     /locus_tag="PK717_00555"
     CDS             1589..1876
                     /gene="groES"
                     /locus_tag="PK717_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOR46197.1"
                     /translation="MNLRPLGDRVIVKPLDEEETKKGGLYIPDTAKEKPVRGEVIAVG
                     SGKILDNGVRVPMDIKVGQKVIYAKYGGTDIKIEGDNLVILSERDLLAVVE"
     gene            1890..3512
                     /gene="groL"
                     /locus_tag="PK717_00560"
     CDS             1890..3512
                     /gene="groL"
                     /locus_tag="PK717_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962963.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOR46198.1"
                     /translation="MAAKKIIFGEDARKKLSNGINTLADAVKVTLGPRGRHVILEKKY
                     GSPVASDDGVSIAKDIELPDPFENLGAVMAREVASKTSDVAGDGTTTATVLAQHLVNE
                     GMRNVAAGANPIAIKRGMDKAVKEAIKVIKSMGKPVETKEDIEKVATVSSKVPEIGKT
                     IAEAMDKVGRDGVITVEEHQGFELKLDIVEGMQFDRGYVSPYMVTDAERMETTLEKPR
                     VFITDKKLSSMQEIFDLVTKGVLSQHEPFVIIAEDVEGEALTFLVLNKLKGALNCVAV
                     KAPGFGDRRKEMLKDIAILTGGEVVASDLGIKVESVTQDMLGTCERVKVTKDTTTILG
                     GKGNSSDIKARIEQIKAQIAETKSDYDKEKLQERLAKLAGGVAVIKVGAATETEMKEK
                     KHRVEDAVAATKAAVEEGVVAGGGVTLLNVAKHLGKMQLSGDEATGVSIVKKALEEPI
                     KVIAENSGLEGSVVIEKIRQLPEGHGFDAEAMKYGDMVEFGIIDPVKVTRAGLENASS
                     IASMVLTTESLVVEIPEEKKVPAAPPNPYGEY"
     gene            3884..4441
                     /locus_tag="PK717_00565"
     CDS             3884..4441
                     /locus_tag="PK717_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOR46199.1"
                     /translation="MSQKEVTRRELLKVLLGLPLLGLLAGAISPVLRLLKPFGKLGLA
                     IPPPSVARVVLSSMREPMLAGGGTSFADVEANYNSMKEMLGYSFEIVTIDTSIPTKPV
                     KISNPGILIKDKEGKLHAWDAKCTHLACVIYWDSVSNNWHCRCHDGFFDPVTANVIAG
                     PPPSALKPWKVEWDQGSGAITVEKV"
     gene            4444..5508
                     /locus_tag="PK717_00570"
     CDS             4444..5508
                     /locus_tag="PK717_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOR46200.1"
                     /translation="MSPSKSYSPFKPLNFLINGTLGIFDVLKAGFESINILNSLGSIT
                     FLLLFIQILTGFVLSFFYMPSLDQAQKSIMYIMSDKIPYGLFIRTLHRYAADAMILFA
                     AAHMLRKLFAQRHTSTRSVGWFVGVALLVFTVLITITGYILPMDGRASEILKFFGRGQ
                     ITEVALLVIYRAFHIGAPILVFALLVWHFARISRPPVIPTFALSLIFLGVLAVIVGLF
                     PIAGKTDLKAGKYVFDWIGLFTIRLGSPTIAASAWGFLIAVLIALPFFGRKIKTAAVV
                     DPVRCSGCFTCITLCPKNAITQKKFNVSRNKTKDVAFVVRKKCQACGICVAGCLPQVI
                     DLEGFENKVILEEVKGLCLQ"
     gene            5496..5903
                     /locus_tag="PK717_00575"
     CDS             5496..5903
                     /locus_tag="PK717_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOR46201.1"
                     /translation="MPSIEDLPIKRKIIVFHCESALKGKDLENHKDKFPEVGFVKLSC
                     TGRLNPVVAFKALVEGVGAIIIYGCPLNDCHNFDGNMFAKRRIFAAQSVAEALGVDPE
                     RLSYIQKDPLDYGVLTREISAMKRKLDSMGVEA"
     gene            5900..6895
                     /locus_tag="PK717_00580"
     CDS             5900..6895
                     /locus_tag="PK717_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46202.1"
                     /translation="MMTEKTLAYFRNIVKEAIKSGKIAGFLGWREVADMPGKTRAFVA
                     TTHEEADLLIFNSFSRENLSRLLGGKTFYVPELKKGQKFGVMVKGCDSRNLLANVAEN
                     KVDRDNLWVIGVKCPGTIDIDLVRSGLDSDIKNITDDGTTLHVEMLDGKKVDLVKSDY
                     YDPRCLRCPYTSALEVDELLPDDMPKKPTKNWEKWMESYLQKPFEERRAYIISQLSRC
                     TMCFACRDACPGCYCNTNCIMDHPKLPEPYLQKGTSLASILTYHFIHYFHLSDRCTNC
                     GECARACPENIPLNLITEQIGYMTKTTWDFEPGISDKAKTPLALYKVEEVLGRWA"
     gene            6892..7917
                     /locus_tag="PK717_00585"
     CDS             6892..7917
                     /locus_tag="PK717_00585"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR46203.1"
                     /translation="MKIKKEKMLSLIDESRKTHVAYAPQKNGAVTNFKRIEDIKSIDL
                     KTIRTATPAKEVIFPQTELIYKVEDDKVHQADTSKPAILFGVRPCDARGIQILDNFFL
                     ARDRKDGGKQPFEDPYWKDKRDKTLIIAIACEKPLSTCACSSLGIHPASEAGSDILLV
                     PEGDSYQVIVSTEKGKSFIEANKSVFEDGGDAKATQEKVKKACESMMIFKLDTSKAGQ
                     YMKLYESPIWQKVSQGCISCGVCTFFCPTCHCFEMRDRKTGATSCNKDKCWDSCHFTV
                     YSLEASGHNPRPDVAARYRNKILDKYQYHMTLYGEIACTGCGRCSDLCPAGISLSETL
                     KTLGGAL"
     gene            7917..8762
                     /locus_tag="PK717_00590"
     CDS             7917..8762
                     /locus_tag="PK717_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOR46204.1"
                     /translation="MNPYLPEMATIEKIEQETPNIKTFYIKRDGGNFDYNPGQFCIIG
                     IPGVGDCSISIGNTPTKREHLELTIMKVGAVTEKLHGMKVGDRVTLRGPYGNGFPKDA
                     LKGMNISYMAGGVGFSAISNMLTYSLDKRSDYGKIEILYGAKTPADQVFKNKIFEWAK
                     VKDVEVKRSCDKGDSTWKEDVGVVGQYYDPNFKNRSKLLFEIDPGFKNTAVVISGPPV
                     MIKFTLIGLDKIGFPKDKIFASLEARMNCGCGKCGRCNVGHNYVCQDGPVFTSAQIAA
                     MPAAF"
     gene            8772..10244
                     /locus_tag="PK717_00595"
     CDS             8772..10244
                     /locus_tag="PK717_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOR46205.1"
                     /translation="MDKFANWLDGLTKSWTRWWKIRGLKVNIFDEETPKKKKSNPFYW
                     IGGMLYILLILQVVVGIVLTFYYVPDTSPVEKVDPSKVMYGHGLTIEGAMKEAEEFRV
                     KIDAGEMTEDELLTQEEITQRDEEKAALTTDAEKALVDTKWATITSQRKDILAMEGKT
                     PEEIVADLGVIVSKAYISVADISGNPVTRTIRGIHRYGAYCFIALLMLRWLRMYFNGE
                     FKKPGELTWIIATLVVCISTFSGVTGYLLPFDQRSYWATTVGTQMLDTVDLLPGIGPL
                     GLGAALKFIGLGAHQVGQTTILRFNILHYLLPIAMFLAAELYFIRSRKKRPKLNWVMF
                     AILAIVIIGAILYLPAISEPPPNTVKTPDHILPDWYFLFVYFYLKFLPGAIGPLLTVL
                     FIVFLVALPWIDRREQRNASGRPAYTTLAIGGLTFFLIGSLASYWIPFKDADRNIYIY
                     TILLFNGFVFALAFFLEYVWRRRVIKKKAEKALALGVKSL"
     gene            10241..10498
                     /locus_tag="PK717_00600"
     CDS             10241..10498
                     /locus_tag="PK717_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46206.1"
                     /translation="MKNEWTLIFILALLVGVLSLAQLVQTAKVLGELGPFGFVQLVAF
                     GLIFLAMMFLLVFGMYASEDHRGKVVKRVKLFDKWLSKEKK"
     gene            10495..10752
                     /locus_tag="PK717_00605"
     CDS             10495..10752
                     /locus_tag="PK717_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46207.1"
                     /translation="MKQEKITAVMVLLVLIALGCFLAIMTLELVDGMQSAARIDKVGL
                     ASSLKPLLKYNGLQQWVVVLFGTMLLFTGLAVLTYNYQKKL"
     gene            10752..11777
                     /gene="dprA"
                     /locus_tag="PK717_00610"
     CDS             10752..11777
                     /gene="dprA"
                     /locus_tag="PK717_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOR46208.1"
                     /translation="MERNYWLAISYLLASKPQALGKLKEKGPEEILKDFGKQEIETAI
                     STGLSEIKSSSIEFVCQDDERYPKNLFDLECPPAALFVFGGLPETQDLIAMVGTRKAS
                     PHGIGIARNMAFEFSKTGIVVVSGMADGIDSASHWGALDTDKPTIAILGCGINQARSA
                     TQRELKKRISVKGAIVSEFPPNFPGDRWTFPWRNRIIAALSKACLVIEAPKSSGALIT
                     ARDALDLGRYVLACPGLPGMQNFEGCNKLIKDGALLVDSPSDVLSLYGKTTLYTDESL
                     SETEKAILLACAVPCRIDEICDKTDLNAEALLGIITVLDIKGLVIKLPNGFYASRSFS
                     DTKTGKN"
     gene            complement(11745..>12810)
                     /locus_tag="PK717_00615"
     CDS             complement(11745..>12810)
                     /locus_tag="PK717_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOR46209.1"
                     /translation="DVTNEDGEKLDSGVVLVFKSPNSFTGEDVVEIQVHGSQAVISKI
                     LLILAQLGARQAKPGEFSMRAFMNGKAGLADLELANALANSTDASRGLQPQLEEIRND
                     LLFIISSLEASISFPNDVSNEDSAKKQTTNLLFKIKSLLGSCSKPGLPRVVLAGPQNA
                     GKSTLFNFILGFERTVTSDTAGTTRDWVPQTTKIASRTVELVDGPGLNGSSDAGQSSF
                     RKLFDSADCIVWLDPSAVRPEFADGRFLMVQSQSDREGFPAQRGWLRVSGKYGLGIEE
                     LYKKILQVLGQRPFAATERQMSLLAIIENSVTSALECQTDDAMAFELSSAFQTMSELD
                     GIGASQEVLESIFSSFCIGK"
BASE COUNT         3642 a         2750 c         3035 g         3383 t
ORIGIN      
        1 cgcttcgcaa tggcgcgcgc aatggcaaca cgctgctgct ctccgcctga aagcatgttt
       61 ggaaaatccc ttgaacgcag gtttaagcca acgaacccaa ggacttcggc gaccttgtgt
      121 tttatgttcc tgcttgctgc tccttgcact tccatggcaa atgcaacatt ttcccaaact
      181 gtttttgtgg agatgagctt gaaatcctgg aagactatgc cgatccttct gcgcaggagt
      241 gcagttctgt atggtgagag tttcgataca tctttgtttt ctagaaagat cctgcctgaa
      301 gtcggttgtt ctgcggcgta gatgagtttg agaagcgtgc ttttaccaga accagatgaa
      361 cccatgagaa aagcaaattc accttttttt agttcgaaag atacattgga aagtgccatt
      421 ttacccgggt atgactttgt gaccgacatc acatcgagca tttaccactc ctccattttt
      481 gggttcttga atccctcgcc caagacttca tttaaggggc cgatcatgat aaaggatttt
      541 ggatcggttt caaggatgat ttttttggcc tccgcaagct gtgacctgtg aacggccatt
      601 accagcattt ttttgtcatc tccagtgaat ccacccttga cgtctattat cgtgaatccc
      661 ctgcccaggt gcaatatcaa attttctctt atttgttccg tttctgttgt gataatgctc
      721 atgcattttg taaaagagat gccttcctgg acagcatcta ttacgaagct gcttatgaca
      781 agggcaacca tggcatatat aaacaggttt gcgttctgga aataaaatgc gagccctatt
      841 ataaccaccg aatcaacaat caggacagtt tggccgaatg gaaatttggt gtagtagtgg
      901 attacttgcg atataagatc ggttccgccg gcacttgccc tgtttttgaa tgtcaggcct
      961 atccccagtc cattgaggat tccgccgaat accgaagcaa ggagcatatc ggatgtgagc
     1021 tttgtgggaa gatatttatt gataagaaat atgaacaaat cagtaaaaac aaacgaaatg
     1081 aaagatatca caagggtcct ggtaatgtat ttgtggccca tttttttcca tgccaaacca
     1141 agccatggca gattgagcaa gaagtatgaa aagcctaccg gcaaaaaacc atttgttaaa
     1201 gaattgacaa tgatggcaac cgctgtcatt ccaccaggtg ctattttgtt tggtacaagg
     1261 aacaaggcat aggaaagcga ctcgattatt actcctgccg caatcccagc atatcgtttt
     1321 atctcacgtt taaaattgta gctcatcgca aaaaataatt gtagttctgc ttttgcttta
     1381 ggtcaagaaa cgatgaggtt tgcaaatatg tactccgagt gccaattagt gtcgtgattg
     1441 aagtcatagc caataccatt ggtatgttgt ttagattaag cgaaagcgaa acctttggca
     1501 cttgacaaaa ctttgggaca ttgatataaa atgtccttgg ttagcagtta tgtcataaga
     1561 ctgctaaaaa atatgcaagg aggatagcat gaatctaaga ccacttggag acagagttat
     1621 cgttaaacct cttgatgaag aggaaaccaa aaaaggcggg ctttacatcc cggacaccgc
     1681 caaagaaaaa ccagtaagag gagaagttat tgctgttgga tcaggaaaga tcctggacaa
     1741 tggtgtaagg gtcccaatgg acatcaaggt tggccagaag gttatttatg caaagtatgg
     1801 tggaacagac atcaaaatcg agggtgacaa cctggtaatt ctttccgaga gagatcttct
     1861 cgcggtagta gaataataag gagcaaaaaa tggcagcaaa gaaaattatt tttggcgaag
     1921 acgctagaaa aaaactatca aacggcatca atacactcgc tgatgcagtg aaggtaacac
     1981 tcggaccaag aggaagacat gtcattctgg agaagaagta tggttcacca gttgcctctg
     2041 atgatggcgt ttcaattgca aaggatattg aactcccaga tccatttgag aaccttggtg
     2101 ctgtaatggc ccgtgaggtt gcatccaaga cttctgatgt cgcaggcgat ggcacaacaa
     2161 cagccaccgt tcttgcccag catcttgtga acgagggcat gaggaatgtc gccgctggtg
     2221 caaacccgat tgcaatcaag aggggtatgg acaaagccgt aaaggaagcc ataaaggtca
     2281 tcaaatccat gggcaagccg gttgagacaa aagaagacat tgaaaaggtt gcaaccgttt
     2341 cttcaaaagt cccagagatt gggaagacaa tagcagaggc tatggacaag gttggaagag
     2401 acggagtcat caccgtcgaa gagcaccagg gttttgagtt aaaacttgac atagtagagg
     2461 gaatgcagtt cgacaggggc tatgtttcac cttatatggt cactgacgca gagcgcatgg
     2521 aaacaactct tgaaaagcca agagtgttca tcactgacaa gaagctttct tctatgcagg
     2581 aaatttttga tctagtcaca aaaggtgttc tttcacagca cgaaccattt gtcattatcg
     2641 ccgaagatgt cgagggtgag gcccttactt tcttagttct caacaaactc aagggcgccc
     2701 ttaactgtgt tgcagtcaag gcccctggtt ttggcgaccg caggaaagag atgctcaaag
     2761 acattgccat tctcaccggt ggagaagttg tagcctcaga tcttggcata aaggtagagt
     2821 cagtgactca agacatgctt ggaacatgcg aaagagtcaa ggtaaccaag gatacaacaa
     2881 caattcttgg cggaaagggc aattcttctg atatcaaggc aagaattgaa cagataaagg
     2941 cccagattgc agagaccaaa tcagattatg acaaggaaaa actccaggaa cgtcttgcca
     3001 agctggctgg cggtgttgca gtaataaaag ttggtgccgc aaccgagacc gagatgaaag
     3061 aaaagaagca ccgcgtcgaa gacgcagttg cagcaaccaa agccgctgtt gaagagggcg
     3121 ttgttgctgg tggaggcgtg acccttctta atgtcgcaaa acatcttggc aagatgcagc
     3181 tttctggcga cgaggccacg ggtgtaagta ttgttaaaaa agcacttgaa gagccaataa
     3241 aagtcatagc cgagaattct gggcttgagg gctcggtggt gattgaaaag atccgccagc
     3301 tgcctgaagg tcacggcttt gatgccgaag caatgaagta tggcgacatg gttgaatttg
     3361 gcatcattga cccagtgaaa gtcaccaggg ctggccttga gaatgcttca tcaatcgctt
     3421 cgatggttct cacaacagaa agccttgttg tcgagatccc agaggagaag aaagttccag
     3481 cagccccgcc aaatccatac ggcgaatatt gattttgaca agtttggaac agaaaaccgg
     3541 gggccaaaaa agtccccggt tttttatttt tctcaaacct gaattcattc cttatcccca
     3601 caattaaatg tggtatttaa caggatttca ttctttgcat aaaaatatag ttctcttttt
     3661 atattttagg gtagtccaaa aaaatgtatt ggaagttggt tttctttgtt gttgacaaat
     3721 tatttggaaa aacctttctt aaccgaattt tttaaccttt aattatgaag tcatcaaaaa
     3781 accagtgttg ttttgaatgc atcgttaaat aaactctaat ttgacttaaa caaattgtcg
     3841 tattaacttt gagtctatgc aaattctcaa ttcggaggac agcgtgagtc aaaaagaggt
     3901 tacaagacga gaattgctca aggtccttct aggcttgccg ctcctgggct tgctcgcagg
     3961 ggcgatttca ccagtactaa ggttattgaa gccttttggt aaacttggtc tggcaattcc
     4021 accaccatct gtagcgaggg ttgttctctc gagtatgaga gaaccaatgc ttgccggtgg
     4081 cgggacaagc tttgcggatg ttgaagctaa ctacaactcg atgaaagaaa tgcttggcta
     4141 ttcatttgaa attgtcacta tcgacacttc gattccaact aaaccagtaa agatcagcaa
     4201 tccaggaata ttgatcaagg acaaggaagg taaactccac gcctgggatg caaaatgtac
     4261 ccatcttgca tgcgttattt actgggactc ggtttcgaac aactggcact gtcgttgtca
     4321 tgacggtttc tttgacccag tgaccgcaaa tgtcattgca ggcccgcctc catcagccct
     4381 taaaccctgg aaagtggaat gggatcaagg ttctggtgca ataacagtgg aaaaggtgtg
     4441 actatgagcc caagcaaatc ctatagccct tttaagccgt tgaattttct cataaatggg
     4501 acattgggaa tttttgatgt cctaaaggca ggttttgaat cgatcaacat tctcaacagc
     4561 cttggctcta taaccttcct tttgctgttc atccagatat tgacaggttt tgtcctgagt
     4621 ttcttctata tgccgtctct ggaccaggcc caaaagtcga tcatgtatat catgtcggac
     4681 aaaataccat atggcctttt cataaggacg ctccataggt atgccgctga tgcaatgatt
     4741 ctctttgctg cagcacacat gctcagaaaa ctttttgccc agaggcatac aagcaccaga
     4801 tcggttggat ggtttgtcgg cgttgcattg ctggtgttca cagtacttat aaccataaca
     4861 ggctacattc ttccgatgga tggaagagca tctgaaatac tcaaattttt tggaagaggc
     4921 cagataacgg aggttgccct gcttgtcatt tacagggcat ttcatattgg agctccaatc
     4981 cttgttttcg ctcttcttgt atggcatttt gccaggattt ccagaccgcc agttatccca
     5041 acatttgccc tctcattgat tttccttgga gttcttgccg tgattgttgg cttgttccca
     5101 atcgctggca aaacagatct caaggctgga aaatatgttt tcgactggat agggctgttc
     5161 actatacgac tcggcagtcc caccattgca gcaagtgcct gggggttcct catcgcagtt
     5221 ctgattgcgt tgccattctt tggcaggaag attaaaacag ccgctgttgt tgatcctgtc
     5281 cgttgttctg ggtgctttac ctgcatcacg ctttgcccaa agaacgcgat aacacaaaag
     5341 aaattcaatg tatccaggaa caagacaaaa gatgttgctt ttgttgtaag aaaaaaatgc
     5401 caggcttgtg gaatctgtgt tgctggctgt ttaccgcagg tcattgactt agaaggtttc
     5461 gagaacaaag tcatactcga ggaggtaaaa ggcctatgcc ttcaatagaa gatctcccga
     5521 tcaaaagaaa aataatagtt ttccattgtg aatcggctct gaaaggcaaa gaccttgaaa
     5581 accacaaaga caaatttcct gaagttggct ttgtaaagct ttcctgcaca ggcagactca
     5641 atcctgttgt tgccttcaag gcccttgtgg aaggtgttgg agcaatcatc atctatggtt
     5701 gccctctcaa cgattgccac aattttgatg gaaacatgtt cgcaaagcgc aggatttttg
     5761 cagcacagtc agttgcagag gctctcggtg ttgacccaga gaggctctcc tatattcaaa
     5821 aagatcctct tgattacggt gttttgacaa gagaaatttc tgcaatgaag cgcaaactcg
     5881 acagcatggg ggtggaagca tgatgacaga aaaaaccctc gcctatttca ggaatattgt
     5941 aaaagaagcc ataaagtcag ggaaaattgc tggtttcctc ggctggaggg aagtcgctga
     6001 tatgcctgga aaaaccagag catttgtcgc tactacccat gaagaagcag accttctgat
     6061 tttcaactcc ttctcaagag agaatctttc gagactcctt ggtgggaaaa ccttttatgt
     6121 ccctgagctt aaaaaggggc agaagttcgg tgtcatggtc aaaggctgtg attctaggaa
     6181 ccttctggcc aacgttgcag aaaacaaagt cgatagagac aatctctggg ttattggcgt
     6241 aaagtgcccc ggcaccattg atattgatct tgtcaggtca ggccttgatt ctgacatcaa
     6301 aaatattact gatgacggaa caacattgca tgtagaaatg ctcgatggca agaaagtcga
     6361 tctggtgaaa tcagattact atgacccaag gtgccttcgc tgcccgtata catcagcact
     6421 cgaggttgac gaactgctac ccgatgacat gccgaaaaaa cctaccaaga actgggaaaa
     6481 gtggatggaa tcttatctcc agaagccttt tgaggagcgc agagcataca ttatttccca
     6541 gctttcaagg tgcacgatgt gctttgcttg cagggatgcc tgtccgggtt gttactgcaa
     6601 taccaactgc atcatggacc atccaaagtt acccgaacca tatctccaga agggaacctc
     6661 tcttgcctca atcttgacat accatttcat ccattatttc cacctttctg acagatgcac
     6721 aaattgtggt gaatgtgcaa gggcctgccc tgagaatatc ccgctcaatc taataacaga
     6781 acagattggc tatatgacaa aaacaacttg ggactttgaa cctggaatca gcgacaaggc
     6841 aaaaacaccg ctggcccttt ataaagttga agaagtgttg gggaggtggg catgaaaatc
     6901 aaaaaagaaa aaatgctctc cctgattgat gaatcgagaa agacccatgt tgcttatgcc
     6961 ccacaaaaaa acggtgcggt cacaaacttc aagaggattg aagatataaa atcgattgac
     7021 ctcaaaacta taaggactgc aacaccagcc aaagaagtga tctttccaca aaccgaactg
     7081 atatacaaag tagaagacga caaggttcat caggcagata cctcaaaacc ggccatcctt
     7141 ttcggggtca ggccatgtga tgccaggggc atccagatac tggacaattt cttccttgca
     7201 agggaccgca aggatggtgg caaacagccg tttgaagacc catactggaa agacaaaagg
     7261 gacaaaactc tcatcattgc gattgcctgt gaaaaaccgc tctcaacatg tgcctgctcg
     7321 tcgcttggaa tccaccccgc ctctgaagct ggctccgaca ttctcctggt tccggaaggt
     7381 gattcctatc aggtcattgt ttcaactgaa aagggaaaat cttttataga ggccaacaaa
     7441 tccgtatttg aagatggtgg agatgccaaa gcgacccagg aaaaagtcaa aaaggcctgc
     7501 gagtcgatga tgattttcaa actggacacc tcaaaagctg gccagtacat gaaactctat
     7561 gaatccccga tttggcaaaa ggtgagccag ggttgcatct cctgcggcgt gtgcacgttc
     7621 ttctgcccaa cttgccactg cttcgaaatg agagaccgca aaactggtgc tacttcctgc
     7681 aacaaagaca agtgttggga ttcttgccat ttcacggtct attcgcttga ggcctctggc
     7741 cacaacccaa ggcccgatgt tgccgcgcgc tacagaaaca aaattctgga caagtaccag
     7801 taccacatga cactctacgg agaaattgcc tgcactggat gcggaaggtg ttctgacctc
     7861 tgtccagcag gaatctcgct ttcagaaaca ctaaagaccc tgggaggtgc gctgtgatga
     7921 acccgtatct gccagagatg gccacaattg aaaaaatcga gcaggaaacg ccaaatatca
     7981 aaactttcta catcaaaagg gatggcggga attttgacta taaccctggc cagttttgca
     8041 taattggaat tccaggtgtt ggcgactgct cgatatccat tggcaacact ccaacaaaga
     8101 gggaacacct tgagctgacc atcatgaaag ttggcgcagt gaccgagaag ctccacggaa
     8161 tgaaagttgg cgacagggtc actttgaggg gcccttatgg aaatggcttc ccgaaggacg
     8221 cacttaaggg aatgaacatc agctatatgg ccggtggcgt tggcttctct gccatcagca
     8281 acatgctcac ctattccctc gacaaaaggt ctgactacgg caagatagag atactctatg
     8341 gtgcgaaaac acccgccgat caagtcttta aaaacaagat ttttgaatgg gcaaaagtaa
     8401 aagatgttga ggtaaagaga tcatgcgaca aaggtgacag cacatggaaa gaagatgttg
     8461 gtgttgtcgg gcagtattat gatccaaatt tcaagaatag gtcaaaattg ctgtttgaaa
     8521 ttgacccagg tttcaagaac actgcagttg taatatcagg gccaccagtg atgattaagt
     8581 tcaccctcat tggtctggac aagatagggt tcccaaaaga caagattttt gcttctcttg
     8641 aggccagaat gaattgcggt tgcggaaaat gtggaagatg caatgttgga cacaactacg
     8701 tctgccagga cggcccggtg ttcacatcag cccagatcgc cgcaatgccg gcggcttttt
     8761 aggaggccag aatggacaaa tttgcaaact ggcttgacgg cttgacaaaa tcgtggacaa
     8821 gatggtggaa gataagaggc ctcaaggtca atatcttcga tgaagagaca ccaaaaaaga
     8881 aaaaatcaaa tccattctac tggattggtg gcatgctcta catcctcctc atccttcagg
     8941 tggtcgttgg cattgtgctg acattttatt atgttcctga cacctcgcct gttgagaaag
     9001 ttgatccttc aaaagtcatg tatggacatg gacttacgat tgaaggggca atgaaggaag
     9061 cagaggaatt cagggtaaaa atcgatgctg gcgagatgac cgaggacgag ctcctcacgc
     9121 aggaagagat tacccagcgc gacgaggaaa aggccgccct tacaacagat gcagaaaagg
     9181 ctcttgttga taccaagtgg gcaacgataa ccagccagcg caaggatata ctggccatgg
     9241 aaggcaaaac cccggaagaa attgttgctg accttggagt cattgtatca aaagcgtaca
     9301 tatctgttgc agacatttct ggcaatccag taaccagaac gataagagga atacacagat
     9361 atggtgcata ttgcttcatc gcgttgctca tgcttcgctg gcttaggatg tatttcaatg
     9421 gtgaattcaa aaaaccagga gaactaacct ggataattgc aacgctggtt gtctgcattt
     9481 caacattctc gggtgtaacc ggttaccttc ttccgtttga ccagaggtct tattgggcaa
     9541 caacagtagg aacgcagatg cttgatactg ttgatcttct gcctggtatt ggtcctttgg
     9601 gcttgggtgc cgcgctcaag tttattggtc ttggtgcaca ccaggttggc cagacgacaa
     9661 ttctaaggtt caatatattg cattacctgt tgccaatcgc catgttcctc gccgcagagt
     9721 tgtattttat caggtcgagg aaaaaacgcc cgaaactcaa ctgggtcatg tttgccatat
     9781 tggcgattgt tataattggt gcaatattgt atttgcctgc aatcagtgag ccaccgccaa
     9841 acacagtcaa gacacccgac cacatcttgc cagactggta ctttctgttt gtttatttct
     9901 atctcaagtt cttgcctggt gccattgggc cattgctgac ggtgttattt atcgtgttcc
     9961 ttgttgccct tccgtggatt gacaggaggg agcaacgaaa tgcctccgga aggccggcct
    10021 acacaacgct tgcgattggt ggcctcacct tcttcctaat aggctctctg gcgtcttact
    10081 ggataccatt caaggatgcc gacaggaata tatacatata cactatactc ctcttcaatg
    10141 ggtttgtgtt tgctcttgcc ttcttccttg aatatgtgtg gaggagaagg gttataaaaa
    10201 agaaggctga aaaggccctt gcacttggag taaaaagtct gtgaaaaatg aatggaccct
    10261 gatttttatc ctggccctgc tggttggcgt gctttcgctt gcacaactcg tccagacagc
    10321 aaaggtgttg ggagaactgg gtccattcgg ttttgtccag cttgtcgctt ttggcctcat
    10381 ctttttggcc atgatgtttc ttctggtttt tggaatgtac gcctcagaag accatagggg
    10441 aaaggtagtc aaaagagtaa agttatttga caagtggctc tcaaaggaga aaaaatgaag
    10501 caagagaaaa taaccgcagt aatggtgctg cttgttctta ttgcacttgg ctgttttctt
    10561 gcgataatga cgctcgagct ggttgatggc atgcagagtg ccgcaagaat agacaaagta
    10621 gggcttgcat caagtctcaa gccgctcctg aaatataatg gtctccagca atgggttgtg
    10681 gtgctttttg gcaccatgtt gctgtttacc ggccttgccg ttctaacata caactatcag
    10741 aaaaaacttt agtggaaagg aattactggc tggctatctc ttatttgctt gcctcgaaac
    10801 cgcaggccct gggaaaattg aaagagaaag ggccagagga aatactgaaa gattttggca
    10861 agcaggagat tgagacagcc ataagtaccg gtctttctga aatcaagtca tcgagcattg
    10921 aatttgtttg ccaggatgac gagagatacc caaaaaacct ttttgaccta gagtgcccac
    10981 cagcggcact ctttgttttt ggtgggcttc ctgaaaccca ggaccttatt gcgatggtgg
    11041 ggacaagaaa ggcctcgcca cacggaatag gaattgccag aaatatggct tttgagtttt
    11101 caaaaacagg gattgtcgtg gtttctggga tggcggatgg gattgattca gcttcacact
    11161 ggggagctct tgataccgac aaacctacaa tcgctattct tgggtgtggg ataaaccagg
    11221 ccagaagtgc gacgcaaagg gagctcaaaa agaggatttc agtaaagggt gccattgttt
    11281 cagagtttcc acccaatttt ccaggtgata gatggacttt cccatggaga aacagaataa
    11341 ttgctgccct ctcaaaggct tgccttgtta tcgaagctcc gaagtcatcc ggtgcattga
    11401 taacagctag ggatgcgctg gatttgggaa ggtatgtact ggcttgtcct ggacttcctg
    11461 gcatgcagaa ttttgagggt tgcaataagc ttatcaaaga tggtgctttg cttgtagatt
    11521 caccttcaga tgttctgtct ttgtatggaa agacaaccct ttatactgat gaatctttaa
    11581 gtgaaacaga aaaagctatt ctgctggctt gtgcggtacc atgcaggatt gatgaaatat
    11641 gtgacaagac tgacctgaat gcggaagcgt tgttggggat aatcacggtt cttgatataa
    11701 aaggacttgt aataaagctc cctaatggtt tttatgcgag ccggtcattt tccgatacaa
    11761 aaactggaaa aaattgattc aaggacttcc tgtgatgcgc cgatgccatc aagctcggac
    11821 attgtctgga aagcagatga aagctcaaaa gccatcgcat catcggtctg gcactcaagc
    11881 gctgatgtaa ctgaattctc aatgatagca agaagtgaca tctgcctctc tgttgcagca
    11941 aaaggtcttt gacccaggac ttgcagtatc tttttataaa gctcttctat tcctaaacca
    12001 tatttacctg acacacgaag ccagcctcgc tgagctggga acccttccct gtcagattgt
    12061 gattgcacca ttaaaaatct cccatcggca aattctggcc ttactgcgga agggtcaagc
    12121 cagacgatac agtcagccga atcaaacaat tttctaaaag atgattgtcc tgcatccgaa
    12181 gagccgttca aaccgggccc gtccacaagc tcgactgttc tactggcaat ctttgttgtc
    12241 tgtggcaccc aatcccttgt tgtgcctgca gtatctgatg tgactgttct ttcaaaacca
    12301 agaatgaaat taaaaagcgt tgatttgcca gcattttgag gtcctgcgag gacaactctt
    12361 ggtaatccag gttttgagca tgagcccaat agggatttta tcttgaaaag aaggtttgtg
    12421 gtttgctttt ttgcggagtc ctcgttactg acatcatttg gaaaactgat ggaggcctcc
    12481 agagacgaaa taataaagag aagatcattc cttatttcct cgagttgagg ctggagtcct
    12541 cttgaggcat ctgtggagtt agcgagggca ttggcaagct caagatcagc aaggccagct
    12601 tttccgttca taaaagccct cattgaaaat tcaccaggtt tggcctgtct tgcacccaat
    12661 tgtgcaagta tcaaaaggat tttgcttatg actgcttgac tgccgtgaac ttgtatttct
    12721 actacatcct caccggtgaa ggaattgggt gacttgaata ctagcacaac cccagaatca
    12781 agtttttcac catcttcgtt agtgacatcc
//
LOCUS       DAOSRI010000011        12618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5011_length_12618_cov_3.914511, whole genome shotgun sequence.
ACCESSION   DAOSRI010000011 DAOSRI010000000
VERSION     DAOSRI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_12618_cov_3.914511"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="PK717_00620"
     CDS             complement(<1..1660)
                     /locus_tag="PK717_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46210.1"
                     /translation="MVGHSRFRFFSLLFVLVCIIAGSSASAASNEWFTFKGGYLRSSY
                     TQVYVEPEVQKVSWKFNSGSSISCSPVTTKNSVYDCTENGYVYCIDANDGTKNWEFPN
                     NSVKDYVDDDGNRPWPNGAIDFQRARLTTPVTVFDNRVYLPYGQMLVVLNADSGKFLF
                     RVDLSGPGHRANITTSPLVSKLHKVIIVGASNGWLYGFDIEGRDPQIRWRMPEPSGTG
                     DQIESSPVLLGNNVYFGSNSRSFYCVELKIPASDKGVRTQDTPELKWVTKLDGPVSSS
                     PASTGSSVNTAKIIVCSETGKIYAISASSGSIIWTYDAGGKIDGSPAISANNRIVIAS
                     ARKIICLDENGKLVWDTLTTKESISTPSIGKDYVYVTSLDGKVYVFSLSNGAQLSVKR
                     TSTAIRSSPAIAYGKLFFGGDDGNIYSLEHGEDPPTLAVSKSNFSYQNVTPNSKIDSN
                     DDLWVENVGGGELDIEISSSRSWITVNPDKLTLQSGKSRTIQMMINTQGLSPGRYSAI
                     IKVITNGGSVSITVDLNIVPVQDTIIVLTVGSTNALVNGSFKKID"
     gene            complement(1638..2171)
                     /locus_tag="PK717_00625"
     CDS             complement(1638..2171)
                     /locus_tag="PK717_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46211.1"
                     /translation="MTPTHYAAQIAEAIEIAKAGGLSIPIVYNCSGFESVEVLKFLEG
                     FIDIYLPDAKYDSDSEALRNSGAVKYVENNRVALVEMYRQVGNLVMDENGIAKRGLII
                     RHLVLPEGRAGTKGVLNWIVKNIPNVHLSLMSQYFPAHRALSEPGLNRNITEDEYEEA
                     LDCLEEVGITNGWTQPF"
     gene            2646..5192
                     /locus_tag="PK717_00630"
     CDS             2646..5192
                     /locus_tag="PK717_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46212.1"
                     /translation="MKKVSIMFLVFLLAIPVTFVQAANNTADGIDKTLQPVSTLIVST
                     TPNVLTAGVVPELVDTATPFTIKVTDSSGTPIDLTQGGSIENKNVWNNLFKDPHPETL
                     DQYYWVRLDLHNDDGTDINNKTLFGIQPIEIDFSMARDGVYIFKGFCANDVGEFKIQV
                     FTPDRKFTGITTVKVRAPLIDYAIFNIEDPEKRVFHTPGDPDFVMTAADNRIYGITAT
                     VQTSDGKLIRGNSKGVQTCSSYTASRLTVGTTMLANFYYSKPHIVLERDPLTGKNLEY
                     IVDMGDRYYINLGIDYNNSGSLEETNREIYDIAGFRVRNWNPSTRRFEETNYKTFYNT
                     TCTKWDDGTYATGYLFDYTDQTTGWGMGCIYNSPYAGCYLFPDINEDGKLDYRDSLEL
                     DFGGKTMFYIFANDVCGITCLVGANHYGQSDFAGRGPINETDPQDVRKRYRPDGTFML
                     DMDGWVAFNLASGRQSTSQISATVSVSPEKLEVGKPAHIEVTVTTKNDNKPIDRARVQ
                     VMGGGILETQYTNELGKANFDVMPDKPGKLTINIAAGNFGSFSQDIQVKRDETPPELT
                     INNVPLLFNNPKLKITGQTEPGAKVKVAETDATVGQDGRFFAEIFLGEGDNAITIVAT
                     DTNGNSTRKTITITLDTVPPEMQIEALDPKIIEAKTIKIKGKVNESSKIIIGDKNQNT
                     SAGFEFELECTYGKNTITVEAWDKAGNKAASQTFEFENFRRTTVVLQVGSNSMLVNGS
                     TKPIPVPPFIERGTTMVPIRVISEALGATVQYDQASKSIEIVLGEKQIIMQIGTKIMV
                     VNGVKKSLTVAPLIKNGSTFVPFRAIAEAFDCDVKWASETKEITIVRLWY"
     gene            5298..5669
                     /locus_tag="PK717_00635"
     CDS             5298..5669
                     /locus_tag="PK717_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOR46213.1"
                     /translation="MPQPNIGYIRNILNQMAASNPDILGCALVSEDGLPIFSVIPGEI
                     EEDSMSAMSATLYALGERVISDLTSGNLEQVYVKGDQGYVVISSVGQIGTLAVIAKPT
                     AKLGYVLMVLRQVISDLTSSH"
     gene            complement(5658..6491)
                     /locus_tag="PK717_00640"
     CDS             complement(5658..6491)
                     /locus_tag="PK717_00640"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOR46214.1"
                     /translation="MKWKGLRKMLEIDLERAQKLIEGFIHSCVNGASMKGAVLGLSGG
                     LDSALVAAIASKALGPDNVLGLLLPYRQSSKDSIEHAKLVAEKFGIKTKTIDISDMAD
                     GYHEQIDRFRLGNLLARLRMCVIFDQSMAGKGIVLGTSNKTEMLLGYSTWYGDMAAGC
                     YPIGDIYKTQVRALAKYIGVPDVIIEKAPSADLWPGQTDEGEIGNTYERLDQILDMLV
                     DQRLPKEKITQRGFSLQEIETIELRMRKSQFKRVLPPVCKITGRTIDQDFRYIKDWSV
                     G"
     gene            complement(6460..7305)
                     /locus_tag="PK717_00645"
     CDS             complement(6460..7305)
                     /locus_tag="PK717_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOR46215.1"
                     /translation="MKDFRLAVAQIESKFGNLAYNIEKHARVAKELRGSGADMVLFPE
                     LGLSGYSLRDLSYSLAIKPDGKEMRPIIEASKEIAILCSFPEMCPDHVVRISGCLYIG
                     GEIAQIYRKIHLPTHGLFEEARYFGKGNTIKAFDTPFGRFGIMVCRDLWHPEEAYVLS
                     RDGAEMIIACSAIPARVLGKSGFSVKKFFERTTGNIAFTNQVFLASANKVGYEDGVAF
                     FGNSVVFGPDGHELAILPELVEHVQIVDIDSSILARARYNMSLYRESDFGTVLNEMER
                     IEKNA"
     gene            complement(7292..7843)
                     /locus_tag="PK717_00650"
     CDS             complement(7292..7843)
                     /locus_tag="PK717_00650"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOR46216.1"
                     /translation="MKNNALKKIVRGSAIAAAYVVLTGPFAIFSFGAIQVRLAEALTI
                     LPFFWPEAIAGLAVGCFISNLLWSPFGVIDWTLGTFATLLAATLTFLLSRTKKIWLGA
                     IPPIVINGLVVGLYVSVLQGFFDIGIPQGLQGFTATFTNFKIGAYMVVAGQIMLGEAV
                     ALGVLGVPLAYVLKRTGFLNEGL"
     gene            complement(7840..8370)
                     /locus_tag="PK717_00655"
     CDS             complement(7840..8370)
                     /locus_tag="PK717_00655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR46217.1"
                     /translation="MMPKKISEENLFDGILFSVQRETYQDEKEYIREFIRTKFEAAVV
                     LPITGDGKVILVKQFRAPVRSDLIEVVAGKIEEGEIPVEAAFRELEEETGYEAGNMVS
                     LGKAFVTPGISTERSHYFLARDLKKGIPNPDEDENVHPIEVPMAEFERMLTDGRIEDA
                     KSLVIYGLYKLWRGEQ"
     gene            complement(8367..10028)
                     /locus_tag="PK717_00660"
     CDS             complement(8367..10028)
                     /locus_tag="PK717_00660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOR46218.1"
                     /translation="MSRNEPHSVLITPLGGLGEIGKNLTVIEWENNAVIIDAGFKFPD
                     ADLPGIDKVIPDLSYLDTISKKIRAVLLTHGHADHIGGLRYVLEKVKVPVYGTELTIG
                     LADEVLPGSVRPVDFRTVQFGKHYFIAGVRTEFIRVNHSIPDGAAIALHFPAGIVLHT
                     GDFKIDLNPIDGNPMDLQRLGDLGLKGILCLMADSTNADEPGYTASESTVGKTFEQLY
                     KFAPGRIVVATFATNIHRLQQIVDTTVKFGRKLIIDGKSIVDTIKVAQRLGYFNMQDS
                     MIITPEQANKMPPEKVAIVTTGTQGEPMSGLTKIANATHPKIHIQKTDTVLISADPIP
                     GNESLVSRTVSGLLKLGANVYYRNVDGVHVSGHASQEEMKIVLALARPKYFIPLHGEY
                     KQLWFHAKIAENIGINKENIFICDNGDQIQLSEKVGKIVKKVPAGAVYIDGSSVGDIG
                     TVVLKERSKLARDGFMTISVAVSKDTREILAGPDITSQGFIYMKESAETIEVVKQAVI
                     NIVKNWKGEGSSTSELKRQIREKLSDVLLETTRRRPMILPTVLQI"
     gene            complement(10028..10606)
                     /locus_tag="PK717_00665"
     CDS             complement(10028..10606)
                     /locus_tag="PK717_00665"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOR46219.1"
                     /translation="MTDAEVILNEITRQVSTCRKCSLGVMRKNPVPGIGNYDASIMFI
                     GEGPGANEDEKGEPFVGAAGQLLDKLLLMIGLKRSEVFITNIVKCRPPQNRTPQQDEI
                     ESCKPYLMAQIAAIMPKIIITLGAPATQTMLSKQMSISVTHGKLQLKEGLRFLPMYHP
                     AAYLHKRDPELLEAMKRDFRELRLILDQTIIK"
     gene            complement(10599..11822)
                     /locus_tag="PK717_00670"
     CDS             complement(10599..11822)
                     /locus_tag="PK717_00670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR46220.1"
                     /translation="MKLVVDTMPGFGSVALGLFMPVGAYTESEDQQGLSHFLEHLVFK
                     GTPKRSAGDIAIEIDGTGGELNAYTSVELTSFYAKVLVDDVDLALDLLFDVTYNPSLQ
                     DDSINLERGVVLSEIAEFMDAPDEIASVRAFQAAWGTHPLARSVLGFPEVIKEVSPNN
                     VRKYHKDHYRPENTVLSACGGITYEQLSAKLEKIGIPHEPLNGKTSYIEDRPEFKPNK
                     LCMDRDSEQVYFTYVWPGPTLCDAEMAEALVSSVIIAGSISSRLFQRLREKEGLVYNI
                     SMMSSFSKSAGLLGVYGAVPKENYQKSRDILLYELNNLRNHGVTEEELERAKRMIKGS
                     TTLSLESNMARMDRSGKFGLLLGQVPSLEETLNRIDSLGIKSFNNYIKQNIPEDYAIS
                     LVGKGICDQKGFVSD"
     gene            complement(11819..>12618)
                     /locus_tag="PK717_00675"
     CDS             complement(11819..>12618)
                     /locus_tag="PK717_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOR46221.1"
                     /translation="IAMGLITSNDKIAILTDIQGVEDALGDMDFKVAGTTEGITGLQM
                     DIKIKGIDMNIIRRALAQAREARMFILGLMGQCLPGPRSELSPNAPRVYTMAIDTEKI
                     REVIGPGGKVIKKIIEDTGCKIDIEQDGRVFITAPNEEAGMKAKGIISEITEDVTVGK
                     IYLGRVTGIRDFGAFVDIGKGGKEGLLHISEIANYRVAKVEDELKIGQEVMVKVKKID
                     ETGRISLTRKGLLETDSGTIPPAIDGDEDHGREDDHHGKRHGKDRRK"
BASE COUNT         3382 a         3085 c         2664 g         3487 t
ORIGIN      
        1 catcaatttt cttgaaggaa ccattcacaa gagcgtttgt ggagccaaca gtcaaaacga
       61 tgattgtgtc ctgaaccggt actatattga gatctacagt tattgagacc gaaccaccat
      121 tggttataac cttaatgatc gccgagtatc tgccaggaga aagaccctga gtatttatca
      181 tcatctggat cgtgcgggat tttccactct ggagggtcaa cttatcagga tttactgtta
      241 tccatgatct tgatgacgat atttcgatat ccaattcacc gccaccaaca ttttcaaccc
      301 aaaggtcatc attgctgtca atctttgagt tgggcgttac attctggtaa gaaaagtttg
      361 attttgaaac agcaagagtt ggtgggtcct caccatgttc cagcgaatag atgtttccgt
      421 catccccgcc aaaaaagagc ttgccgtagg ctattgctgg tgatgatctt attgcggtac
      481 tagttctctt gactgacagc tgggcaccgt ttgacaggct gaaaacatac actttaccat
      541 caagggaggt aacataaaca taatctttac cgatagatgg cgttgagata gattcttttg
      601 ttgtaagcgt gtcccagaca agtttaccgt tttcatccag gcaaatgatt tttcttgccg
      661 aagcaattac gatcctgttg ttggcagaaa tggccggtga gccatctatc ttgcccccag
      721 catcgtaggt ccaaattatg gaacccgaag aagctgagat tgcatatatt ttcccagttt
      781 ctgagcaaac aattattttt gctgtattga cacttgagcc agttgatgcg ggtgagcttg
      841 aaacgggccc atcaagcttc gtcacccatt tcagttctgg agtgtcctgt gttctaacac
      901 ctttgtcgga agcagggatc ttcaattcaa cacaatagaa actcctcgaa tttgatccaa
      961 aatacacatt gtttccaaga aggacaggac tgctttcaat ctggtccccg gtgcctgatg
     1021 gttcaggcat tctccacctt atctgcgggt ccctgccttc aatatcaaat ccataaagcc
     1081 agccattgct tgcaccaaca ataatgacct tgtggagctt tgacaccaaa ggagatgttg
     1141 taatgtttgc cctgtgccct gggcccgaaa gatcaaccct gaaaagaaac ttccctgaat
     1201 cagcattgag aacaacgagc atttggccat aggggagata gaccctgttg tcaaagactg
     1261 ttactggggt tgtgagtctt gccctttgaa aatctattgc gccatttggc catggtctat
     1321 taccatcgtc atcaacataa tcctttacgg aattattggg gaactcccag tttttggtac
     1381 catcattggc atcaatgcaa tatacatagc cattttcggt gcaatcataa accgagttct
     1441 tagttgtgac tggcgagcag gatatggaag aaccagaatt aaatttccag cttacctttt
     1501 ggacttctgg ctcgacatag acttgagtat aggaacttct aaggtagcca cccttgaaag
     1561 taaaccattc gttggaagcc gcactggcac ttgagccagc aattatacaa acaaggacaa
     1621 aaaggagtga gaagaaccta aaacggctgt gtccaaccat ttgttatccc cacctcctca
     1681 aggcagtcaa gtgcctcttc atattcatct tctgttatgt tcctgtttaa acctggctct
     1741 gaaagcgctc tgtgagcagg aaaatattgg ctcatcagcg aaagatggac gtttggaata
     1801 ttttttacaa tccagttcaa aactcctttt gttccagccc ttccctccgg aagaaccaga
     1861 tgccttatta tgagacctct ctttgcgatc ccattttcgt ccattactag attaccaacc
     1921 tgcctgtaca tctcaaccaa agccacccta ttgttctcaa catattttac cgcaccagaa
     1981 tttcttagtg cttcgctatc tgaatcatat ttcgcatctg gaagataaat gtcaataaat
     2041 ccttccaaaa attttaacac ttcaactgat tcaaaaccag aacaattata aacaattgga
     2101 atagaaagtc cacccgcttt agcaatctca attgcttctg caatttgtgc agcataatgt
     2161 gttggggtca caaggtttac attgtggcaa cctcttgttt gctgctccag cataatttct
     2221 gcaagctttt caaccgttac ttcatagcca ttatgcaatt gagagatcgg ataattttgg
     2281 caaaacttac acgacattgt gcaatatgtg aaaaagattg tgcctgaacc atttttccca
     2341 gaaattggcg gctcttctcc tggatgagga ccggttgagg atattttcac agtcatgggc
     2401 gcgcggcaat atcccacctc acctttattt ctcattgctc cacattttct ggggcaaagg
     2461 ttgcatggtg aggcaagcct gtagagcttt gaaattttct cgcttctttg caatgcttga
     2521 atcatggccc ttcgaacgag gaattttagc atcatatccg tatcaaaaca agtcccttga
     2581 taaaagattt ctttgaacca aaaaacacca aaaactgtat cttcaagtaa ataagaggag
     2641 gtaaaatgaa gaaagtttca ataatgtttc ttgtctttct tctcgcgata ccggtgactt
     2701 ttgtgcaagc agccaataac actgctgatg ggatagacaa aacactgcag ccagtctcaa
     2761 cgctcatagt gtcaactacg ccaaatgtcc ttactgctgg tgttgttcct gagcttgttg
     2821 acacggcaac accattcaca ataaaagtca ccgattcgtc aggaacaccg attgacctta
     2881 cacaaggcgg atctattgaa aataaaaatg tatggaacaa tcttttcaaa gacccgcatc
     2941 ccgaaacgct agaccaatac tattgggtaa ggctcgatct ccacaatgat gatggcacag
     3001 acatcaacaa taaaacactt tttggcattc agccaattga aattgacttc tcaatggcaa
     3061 gagatggggt ctacatcttc aagggttttt gtgcaaacga tgttggagaa ttcaaaatcc
     3121 aggtatttac gcctgacagg aaatttaccg gcattacaac cgtaaaagtc agagcacctc
     3181 tcattgatta tgccatattc aacattgaag atcccgaaaa aagggtcttt cacaccccag
     3241 gtgaccctga ttttgtaatg acagccgcag acaacaggat ttatggcatc acagccactg
     3301 tccagacttc cgatggcaaa ctgatcaggg gcaactcaaa gggtgtccag acatgcagca
     3361 gctataccgc ctcaagactg actgttggca caacaatgct tgcaaatttc tattacagca
     3421 aaccacacat cgtgcttgag agagacccac tcaccggcaa aaatctcgaa tacatcgtcg
     3481 atatgggtga cagatactac ataaatcttg gtattgatta caacaatagt ggctctctgg
     3541 aagaaaccaa cagggagatt tatgatatag caggattcag ggtaagaaac tggaacccgt
     3601 caaccagaag gttcgaggaa acaaattata aaacttttta caacacaaca tgcacaaaat
     3661 gggatgatgg aacttatgca accggctatc tctttgacta caccgaccag acaaccgggt
     3721 ggggaatggg gtgcatctac aattccccat acgctggctg ctatctgttc cctgacataa
     3781 atgaggatgg caagctggat tacagagact ctcttgaact cgattttggc ggaaaaacaa
     3841 tgttctacat ctttgccaat gatgtttgcg gaataacttg cctcgttgga gcaaaccatt
     3901 acgggcaatc tgattttgcc ggtagaggac caataaatga aacggatccg caggatgtca
     3961 gaaaaagata caggccagat ggcactttca tgctcgatat ggacggatgg gtggcattca
     4021 acctggcgtc tggcagacaa tcaacctcac agatcagcgc aactgtttcc gtttctccgg
     4081 aaaaactcga agttgggaaa ccagcccaca ttgaagtcac tgtcacaaca aaaaatgaca
     4141 acaagccaat cgacagggca agggtccagg tcatgggtgg aggcatcctc gaaacacaat
     4201 acacaaacga acttggaaaa gccaattttg atgtcatgcc agacaaaccc ggcaaattga
     4261 caatcaatat cgctgcaggc aactttggtt ctttctcgca agacatacag gtaaaaaggg
     4321 acgaaactcc accagaactc acaattaaca acgttccgtt gcttttcaat aatccaaaac
     4381 tgaaaatcac agggcaaact gagcctggcg caaaagtgaa agtcgcagaa acggatgcaa
     4441 cagtaggtca ggatggcagg tttttcgctg aaatattcct tggagaagga gacaatgcca
     4501 taacgatagt tgcaactgat accaacggca attccacacg caaaacaata accatcacat
     4561 tggacacggt cccgcccgaa atgcaaattg aggctttgga tccaaaaata attgaagcca
     4621 aaacgataaa gataaaaggt aaggtgaacg agtcatccaa aattatcatt ggtgacaaaa
     4681 accagaatac ttctgctggt tttgagttcg agcttgagtg cacatacggc aaaaacacca
     4741 tcacagttga agcgtgggac aaagccggca acaaggccgc ctcacaaaca ttcgagtttg
     4801 aaaacttcag aagaacaacc gtcgtgctcc aggttgggtc aaactcaatg cttgtcaatg
     4861 gatcaacaaa accaatcccg gtacctccct tcattgagcg aggaacaaca atggtgccca
     4921 tcagggtcat ttcagaggcc cttggggcca cagtccaata cgaccaggca tcaaaaagca
     4981 tcgagatcgt ccttggcgaa aaacaaataa ttatgcagat aggcacaaag ataatggtgg
     5041 tcaacggagt aaaaaagtca ctcactgttg cgccgctcat aaagaacggc tcaacgtttg
     5101 tacctttcag ggcaatcgcc gaagcctttg actgtgacgt gaaatgggcc tccgagacaa
     5161 aggaaataac aatagtgagg ctctggtact aaaaaatcag ttggaaccag ttaaaaggcc
     5221 ccaggaattt ttccctgggg ccttttttgt ttgggtgtga ttgactaata aagcataacc
     5281 gctatcattt tgttgaaatg ccccaaccaa atattggcta tattagaaac attctgaacc
     5341 agatggctgc aagcaaccct gatattttgg ggtgcgcact ggtttctgaa gatggtcttc
     5401 cgatattctc cgtaatacct ggcgagatcg aagaagattc gatgtcggcc atgtccgcca
     5461 ccctttatgc gcttggtgaa agagtcatca gcgaccttac aagcggaaac cttgaacagg
     5521 tctatgtgaa gggtgaccag ggctacgttg taatctcatc agttgggcaa ataggcacgc
     5581 tggcagttat cgcaaaacca acagcaaagc tgggttatgt attaatggtg ctcagacagg
     5641 tcatctctga cctgacatca tcccactgac cagtctttga tatatctaaa atcctgatcg
     5701 attgttcttc cggttatttt gcacactggt ggcaacaccc tcttgaactg cgacttgcgc
     5761 atcctcagct caattgtctc aatttcttgt aagctaaaac cacgctgtgt gattttttcc
     5821 tttgggagcc tctggtcaac cagcatgtca agtatctggt caagtctctc atatgtattg
     5881 cctatctcac cctcatcagt ttgccccggc cacaagtccg cagaaggggc cttttctatt
     5941 atcacgtcag gtacaccaat gtactttgcc agcgctctca cctgggtctt gtatatatcg
     6001 cctatgggat agcaccctgc ggccatgtcg ccgtaccacg ttgaatagcc caggagcatt
     6061 tctgttttgt tggaagtccc caaaacgatg cctttgccag ccattgactg gtcaaatatc
     6121 acgcacattc tgagccttgc cagaagattg ccaagcctga acctgtcaat ttgttcatgg
     6181 tagccatcgg ccatatcgga aatatcaatg gttttggttt ttatgccaaa tttctccgca
     6241 actagcttgg catgctctat cgaatccttg cttgactgcc tgtatggaag caggagcccc
     6301 aagacattat ctggcccaag ggcctttgag gcaattgcag ccacaagggc cgaatcaagg
     6361 cctcctgaga gtcctagaac agcacccttc attgaagcgc catttacaca ggagtgaatg
     6421 aacccttcaa tgagtttttg cgctctctca agatcgattt caagcatttt tctcaatcct
     6481 ttccatttca ttaaggaccg ttccaaaatc gctttccctg taaaggctca tgttgtatct
     6541 tgcccttgcg aggatcgatg aatcaatatc gacgatctgg acatgctcta caagctctgg
     6601 cagaatcgcc agttcatggc catccggacc aaaaacaacg gaattgccaa aaaatgccac
     6661 accatcttca tacccaacct tgtttgcact cgccagaaaa acctggttag taaaagcaat
     6721 attgccagtt gttctttcaa aaaacttttt taccgagaaa ccactttttc caaggaccct
     6781 tgccggtatc gccgaacagg caattatcat ctcggcgcca tccctggaga gtacataagc
     6841 ttcttccggg tgccagagat cacgacacac cattatccca aacctgccaa atggcgtgtc
     6901 aaaagccttt atggtattgc cttttccaaa atacctcgcc tcctcaaaca ggccatgcgt
     6961 tggcaaatgg atttttctgt atatctgggc aatttcaccc ccgatataga ggcagccgct
     7021 gattcttacc acatggtccg ggcacatctc cggaaaactg cataaaatgg caatctcttt
     7081 tgaggcctca attatcggac gcatctcctt gccatcaggc ttgatggcaa gactatacga
     7141 gaggtccctt aaactgtagc ctgaaagccc cagttcagga aaaagcacca tatcggcgcc
     7201 actaccacgc aattcctttg cgactctagc atgtttttct atgttgtatg cgagattgcc
     7261 aaattttgat tcgatttggg ctactgccag cctaaagtcc ttcattcaaa aaaccagtcc
     7321 tttttagtac atatgcaagt ggaacaccaa gaacgccaag ggccaccgcc tcaccaagca
     7381 ttatctgccc ggcaacaacc atatacgccc cgattttaaa attggtgaac gtggctgtaa
     7441 acccctgtaa tccttgcggg attccaatat caaaaaatcc ttgcaaaaca gacacataga
     7501 gtccaaccac aagcccattg atgacaatcg gagggatggc acccagccat attttttttg
     7561 ttctggacag caagaatgtg agtgttgccg ccaaaagcgt ggcaaaagta ccaagtgtcc
     7621 agtctatgac accaaatgga gaccagagca gattggagat aaaacatccg actgcaagcc
     7681 cggcaatagc ctctggccag aaaaatggca gtatagtgag tgcttcggca agtcttacct
     7741 gtatggcacc aaaagaaaat atagcaaaag gcccagttaa aacaacgtat gcagcagcaa
     7801 tggccgatcc tctgacgatc tttttaagtg cgttattttt cactgctcac ccctccatag
     7861 tttgtataat ccataaatga caagcgattt tgcatcttcg atgcgcccat cggtaagcat
     7921 tctttcaaat tctgccatcg gcacttctat tgggtgcaca ttttcatctt catcaggatt
     7981 gggtattccc tttttgaggt ccctggccaa aaagtaatgc gatctttcgg tacttatgcc
     8041 tggcgtgaca aaagccttgc caaggctgac catgttgcca gcctcatagc cagtttcttc
     8101 ttcaagctcg cgaaaagccg cctcgacagg aatctcgccc tcttcgatct ttccagcaac
     8161 aacttcaatc aagtcactcc ggacaggggc cctgaattgc ttcaccagaa taaccttgcc
     8221 atcgccagtt attggcagaa caaccgcggc ctcaaatttg gttcttataa actcccttat
     8281 gtactctttt tcatcctgat acgtttctct ctggacggaa aacaggatgc cgtcaaacag
     8341 gttttcttct gaaatctttt ttggcatcat atctgaagaa ccgttggaag tatcatcggc
     8401 cttctccttg tagtttccag caaaacatca gacaatttct cccttatctg ccttttcagc
     8461 tcacttgttg atgagccttc gcccttccag tttttcacta tgttaatgac agcctgcttt
     8521 acaacttcga ttgtctctgc cgattctttc atgtagatga agccctggct ggttatgtct
     8581 ggccctgcca aaatctccct ggtgtccttt gaaactgcaa cagatattgt cataaaacca
     8641 tcccttgcaa gctttgatct ttccttgagg acaacagtgc ctatgtctcc aactgaactc
     8701 ccgtcaatgt atacggcccc ggcaggcact ttctttacta ttttccctac cttctccgag
     8761 agctgtatct ggtcaccatt gtcgcaaata aagatgttct ctttgttgat tccgatgttc
     8821 tctgcgatct ttgcgtggaa ccagagctgc ttgtattcgc catggagcgg gatgaaatac
     8881 tttggtctgg caagcgccag gactatcttc atttcttcct gtgatgcgtg gccagagaca
     8941 tgcactccgt caacattgcg atagtagaca tttgcaccga gtttcagcaa cccggaaaca
     9001 gtccttgaaa caagactttc attgcctgga atggggtcag cagaaatgag aacagtgtca
     9061 gttttctgta tgtggatttt tggatgggtg gcattggcaa ttttggtcag gccagacatt
     9121 ggttcaccct gtgttccagt tgtaacaatg gcaacttttt ctggaggcat tttgtttgcc
     9181 tgctctggcg tgattatcat gctgtcctgc atattgaaat agccgagcct ctgcgcgact
     9241 ttgatcgtat caacaatgct ctttccatct atgatgagct tgcgtccaaa ttttactgtt
     9301 gtatcgacaa tctgctggag cctgtgaatg tttgtggcaa aagtggccac aacaatcctg
     9361 ccaggagcaa acttgtacag ttgttcaaag gtcttgccaa ctgttgattc tgaagcggtg
     9421 tagcccggct catcagcgtt tgtcgaatca gccataaggc acagtatacc cttcagccca
     9481 aggtccccga gtctttgaag gtccatggga tttccatcaa tcggattgag gtcaattttg
     9541 aaatcgccag tgtgtaggac aatcccggcc ggaaagtgga gtgcgattgc cgcaccatct
     9601 ggtatggagt gattgaccct tatgaattct gttctgacac cagcaataaa gtagtgcttt
     9661 ccaaattgca ctgtcctgaa atctacgggc ctaactgagc ctggaagcac ctcatcagca
     9721 agcccgatgg tcaattccgt cccgtaaaca ggcactttga ccttttcaag aacatatctg
     9781 aggccaccga tgtggtcggc gtgtccatgg gtcaggagta cagcccttat cttcttggaa
     9841 atagtatcca gatatgaaag atccggaata actttgtcaa ttcctgggag atcggcatct
     9901 gggaatttga aaccagcatc aattattaca gcgttgtttt cccactcgat aacggtcagg
     9961 tttttgccaa tctcgccaag accgccaagc ggcgttatca agacagaatg tggttcattt
    10021 ctggacatta ttttattatt gtttgatcca ggatgagcct tagctccctg aagtctcttt
    10081 tcattgcttc cagtagttcc gggtcccttt tgtggaggta tgctgccgga tggtacattg
    10141 ggaggaatct gagtccctcc tttaactgga gctttccgtg tgtcacgctt atggacattt
    10201 gctttgacaa cattgtttgg gttgccggtg ccccgagcgt gattattatt tttggcatta
    10261 tggccgctat ctgggccatt agatatggtt tgcatgattc tatttcgtcc tgttgtggcg
    10321 tcctgttttg aggaggcctg cattttacta tatttgttat aaaaacttcg gaccttttca
    10381 gtcctatcat caagaggagt ttgtccagga gctgtccagc agccccaaca aacggttcac
    10441 ccttctcatc ttcgtttgca ccaggacctt caccaatgaa cattattgat gcatcatagt
    10501 ttcctatgcc tggtactgga ttttttctca ttacgccaag actgcactta cggcaggtag
    10561 aaacctgtct ggtgatctca ttcaagatca cttcggcgtc agtcactcac aaaccccttt
    10621 tggtcacaaa tgccctttcc tacaagtgaa atggcatagt cttccggtat gttttgcttt
    10681 atgtagttat tgaaactctt tattcccaag gagtctattc tgtttagcgt ttcttcaagt
    10741 gacggcacct gcccaagaag tagcccgaat tttccgctgc ggtccatcct tgccatgttg
    10801 ctttcgaggg acagggttgt cgaacccttt atcatcctct ttgccctctc gagctcctct
    10861 tcagttacac catggttgcg aaggttgttc agctcataaa gcagaatatc acggctcttt
    10921 tggtaatttt cctttggaac agcaccataa acacccagga gcccggcaga tttcgagaaa
    10981 gaagacatca tggaaatatt gtaaaccaga ccctcttttt ccctaagtct ctggaagagt
    11041 ctggagctta ttgaaccggc aataataacg gagctaacaa gcgcctcggc catttctgca
    11101 tcacaaagcg ttggccctgg ccacacatag gtgaaataga cctgttcgga gtccctgtcc
    11161 atgcagagtt tgtttggttt gaattctggc cggtcttcaa tatatgaggt tttcccattg
    11221 agtggctcgt gtggaatgcc aatcttttcg agtttggccg acagctgttc gtaggttatc
    11281 ccgccacagg cagacaaaac agtattttca ggcctgtaat ggtctttgtg gtatttcctg
    11341 acattgttcg gagagacttc ctttattact tccggaaaac caaggaccga tcttgcaagc
    11401 gggtgtgttc cccaagccgc ctgaaacgcc ctgacagaag caatctcatc aggggcgtcc
    11461 ataaattcag ctatctcaga gagaacaacc cctctctcca gattgattga atcatcctga
    11521 agtgaagggt tgtaggtaac atcaaacaag aggtccagcg cgaggtcaac atcatccaca
    11581 agaaccttgg cataaaagct tgtcaattca accgaggtat aggcattcag ttctccacct
    11641 gtaccatcga tttcaattgc tatatcaccg gcactccttt ttggcgtccc tttgaaaaca
    11701 aggtgctcaa ggaagtgcga aaggccctgc tggtcctctg actcagtata ggcacctacc
    11761 ggcatgaaca aaccgagtgc taccgagcca aaacccggca tggtgtctac tactagcttc
    11821 actttctcct gtccttgcca tgcctcttgc catggtgatc gtcttcacga ccatgatctt
    11881 catcaccgtc aatggctggt gggattgttc ctgaatcagt ttccaggagg cccttgcgag
    11941 tgagggagat cctgccagtt tcatcaatct ttttgacttt aaccatgact tcctggccga
    12001 tcttgagttc gtcttcgacc tttgcgaccc tgtaattggc tatttcagag atgtggagga
    12061 gaccttcctt gccacctttg ccgatatcga caaaagctcc aaagtccctt attccggtta
    12121 ctctgccaag ataaatcttt ccgacagtga catcttcggt tatctcggag atgattccct
    12181 ttgccttcat tccagcttct tcatttgggg cagttataaa gacccttcca tcctgttcga
    12241 tgtcaatctt gcagcctgtg tcttcaatga ttttctttat gactttgcca cccggtccga
    12301 ttacctcacg gatcttctct gtgtctatgg ccattgtgta gacccttgga gcatttggtg
    12361 aaagctctga tcttggccct ggaaggcatt gccccatgag accaagaatg aacatcctcg
    12421 cttccctggc ctgtgccaga gcgcgcctga taatattcat gtcgataccc ttgatcttga
    12481 tgtccatctg gaggccagtt atgccttcag ttgttccggc aaccttgaaa tccatgtcgc
    12541 caagggcatc ctcaacaccc tgaatatcgg tgaggattgc aatcttatca ttgcttgtga
    12601 taagtcccat cgctattc
//
LOCUS       DAOSRI010000012        12349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5133_length_12349_cov_3.859932, whole genome shotgun sequence.
ACCESSION   DAOSRI010000012 DAOSRI010000000
VERSION     DAOSRI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5133_length_12349_cov_3.859932"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PK717_00680"
     CDS             complement(<1..1179)
                     /locus_tag="PK717_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR46222.1"
                     /translation="MSIEEIVSQIYRNESGKEMTLIHFEGRKGTFSDIPFCVPETVSA
                     GLRKIGIEKLYSHQSEAIEGVLSGKNVVVATPTASGKTLCYNVPVLSAISKDPAARAI
                     YLFPTKALSFDQQTGLSELVEATGLGVKFHTYDGDTDVGKRKILKVEGSIILTNPDML
                     HAGILPHHTKWVHLFENLKYVVIDELHQYRGVFGSHMANVIRRLKRICTFYGSHPTFI
                     MSSATIANPADLASRIIEDDVEAITEDGSPSGDKYFVVYNPPVVDKQLGLRKSSIFEA
                     ERFASQLVQSGIQTIVFARARTTVENVLKLLKDHASRAGFNPDSIRGYRGGYLPLERR
                     EIEKGLREGKIMGVVSTNALELGIDIGRLEASVMVGYPGTIASMRQQAGRAGRKQQTS
                     L"
     gene            1448..2635
                     /locus_tag="PK717_00685"
     CDS             1448..2635
                     /locus_tag="PK717_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOR46223.1"
                     /translation="MKSVKRGQLVNATVVKVTSDGLLVDVGQKSEGFIPRAEASTRDD
                     VELASTFKTGDVIQCRVVKIGDDDGMIILSKKRADFELVWDRLKEAKDNKEILTTVAT
                     KAVKGGLLVDVGVPAFVPRSQIDLNRNADPTKWVGKELKVRIIEVDRPTRRVVCSQKE
                     VLEEDRERRREEFISNLKVGEIYEGTVVGLVEFGAFVDLGEGVEGLIHLSELSWGGRK
                     TPQQVLKKRQKVNVKVIKIAGDEGRISLSLRQAQPHPWELVPYKYSVGDVVEGTVTRL
                     LKFGAVVELEEGVSGLLHISQVSNHRVERIDTEIKEGQTVQCKILEIRVPDRKIRLSI
                     KATLPEEPRPERQPRKRFNAYEDEEEQYLREFAQESADPSVRLISENDLERENREVFN
                     VNE"
     gene            2670..3395
                     /locus_tag="PK717_00690"
     CDS             2670..3395
                     /locus_tag="PK717_00690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR46224.1"
                     /translation="MKKYLAVIAILALGITFMAACGSKNEETTLPTEQEMSKVTDQYN
                     TDNQTTDQTTTTPTDNATDKTGTENPAEEEDKDMGTTNTDLGKTVVFETNYGAFEISL
                     YSDAPIASKNLIDKANSGFYNGLTFHRIVKGFVIQGGDPLGNGTGGETMGLDPKGLHQ
                     SKRGTISMASADIKTNQSDAQFFINLVDNFRLDEMGFIAFGEVTKGMEVVDKIAEVPC
                     NPGGDGANSSPVSPVLINKVYVK"
     gene            3385..3987
                     /locus_tag="PK717_00695"
     CDS             3385..3987
                     /locus_tag="PK717_00695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR46225.1"
                     /translation="MSNRIVLASLATLALVVSMTSCTGVSLEEKQNEVPPEKPKITFS
                     TTEGDFVIGVLPKNAPNSVNKFLTWCKGFTTPEGQVVACIYNGLQFYRVMPGKFVQGG
                     DPFNNGTGSDAFKIPFEKTEKKLKRGVVCLANDGEGNNNCIFFILLTDTPQFQGKYTA
                     VGEVLQGLDVIDRMSNVPTRPNEPNTPLRNISIIGVKVSE"
     gene            3980..4762
                     /locus_tag="PK717_00700"
     CDS             3980..4762
                     /locus_tag="PK717_00700"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOR46226.1"
                     /translation="MSKIALYCFSGTGNTRLVAQKVKKGLELEGHEVNYFETRDKPTI
                     PMEYDSFGICFPVYDWSPPWPLRELIKSWHGLLNDKPCFIVMDYAGDPVNARLKMMED
                     LEKTGARIFHASEIVMPESWTVVLTPKKIQSMNEWLSKNPQPDPTDLGIEVGKKLKNG
                     DTTPTKKPKYKFSSYSFLIPFYTKTSLKMFYRIDLDKGKCVKCGICAEKCPTGSIKLN
                     SYPSFSYPCASCYACINHCPTQALESWATRGKIRYKPKRVDL"
     gene            complement(4820..6274)
                     /locus_tag="PK717_00705"
     CDS             complement(4820..6274)
                     /locus_tag="PK717_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR46227.1"
                     /translation="MRKYKLDQTRAPLVEKVIEYIGQNRLPFHMPGHMRGGVIPEPLA
                     EWFGEGLYKFDLTEVQGLDYLHTSSGVIGEAQKYAADVFGVRETKFLINGTTVGVQAM
                     MLSSVWNEEKIILQRNSHRCTVGGLILGGLEPAYVMPDYNSDIGEYTSITPTTLSNAM
                     KRHPSAKAVLMTTPNYFGLASDIQTLASMATIKGLRVLVDEAHGAHFMFHPEMPMSAC
                     QCDVDCVTQSAHKTLPTMTQSSLLHVVTARAATDRLKMILVLLQSSSPSYVFMSSLDA
                     ARRYMAIHGQERLTQILEMADWLRDEINKIPGLRCPGSEVVGRQGVVGFDRTKITINV
                     SELGLSGWEAERVLNNEYNVEIELSDADNFLCFLTVGTKWEYVKGLVEVLKKLASKKY
                     DCRPSVKVPLPEIPPLEMLPRKAFYSKSELVPLKQAIGRVAADVVCPYPPGIPTLIHG
                     ELITSQIVDYIEQLDSQKAFIQGLINGEMIKVIA"
     gene            6436..7101
                     /locus_tag="PK717_00710"
     CDS             6436..7101
                     /locus_tag="PK717_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46228.1"
                     /translation="MKLDQNSLSKTLDDFNEHILFGKNIPKKAAEEAAIWIASRQGLP
                     GSYYNMFAPTANDFLETKLFTGEKVASRAATAHILSEEACRTLIKLGAKNLQAKKALE
                     TATEDMLGRIKDNPSGKYCCGTCSVSFWRHLQAGGLSLQEERIANGMGDLKRSRIGNS
                     KWRFYPFFYTLYTLLDIDMKEALEEIKYAAPAMEKSLKSGKADEPYGERRAEIFKKLL
                     AKI"
     gene            complement(7141..7217)
                     /locus_tag="PK717_00715"
     tRNA            complement(7141..7217)
                     /locus_tag="PK717_00715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7181..7183),aa:Pro,seq:cgg)
     gene            complement(7403..7480)
                     /locus_tag="PK717_00720"
     tRNA            complement(7403..7480)
                     /locus_tag="PK717_00720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7443..7445),aa:Pro,seq:ggg)
     gene            complement(7550..7843)
                     /locus_tag="PK717_00725"
     CDS             complement(7550..7843)
                     /locus_tag="PK717_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR46229.1"
                     /translation="MTKPELIDKIASTTKFSKKDCSAMLDATLESIKDAMKTGDKVLL
                     VGFGSFYVRNRKARKGVNPKTRKPLNIPEKKVPAFKPGKDLKSAVHTVHHAKK"
     gene            complement(7863..8864)
                     /locus_tag="PK717_00730"
     CDS             complement(7863..8864)
                     /locus_tag="PK717_00730"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOR46230.1"
                     /translation="MRVCVMGAGAWGTALTTVLASKPIEVILWANTPDLADEIRRNAE
                     NIRYLPGYKLQDKVVIESDPLTASKGIDIMVAAVPGKYLAMAAREFARNLENQTKVVS
                     VVKGIEPEQSLTMSELLSKEWNLDSDKIAVLSGPNFAREVAQKMPTAAVCASKSDELS
                     KQIQQLFSNTYFRVYRSKDVKGVELGGALKNVVAIGAGVIEGLGFGDNTKAALVVRGL
                     EEARRLYKALGAQEATLRGLSCLGDMVATCSSPLSRNKRFGYLLGKGKKPDEARDEIS
                     PDGQAIEGLESLQGLLLLARKYGIELPITSEIDAVSKGSTTPLRAIRRLMKRELKEE"
     gene            complement(8861..10210)
                     /gene="der"
                     /locus_tag="PK717_00735"
     CDS             complement(8861..10210)
                     /gene="der"
                     /locus_tag="PK717_00735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR46231.1"
                     /translation="MENEISSIKPLRAKNLPIVAFIGRPSVGKSTIFNRILRKARTIT
                     DQAMGVTRDRIMEDFTWNGVDFTLMDTGGIAPEASTIQRQVSLQSEMAIGIADVIVFM
                     VDSQIGPTKEDIYVAKMLRKSTKKIILAANKADNKDWTDFNFNQLGFGDPFTVSATSG
                     RNVAELLDLIVESIGKAVEEEQQEQQEEELPPRRIAIAGRPNVGKSSLLNAFLGEKRT
                     TVSDVPGTTRDAVDTLIEYDGTKYILVDTAGIKKRYKYDEQLDYVTIKRSEQAIHRSD
                     LVILVIDGSVGATSDDQHLARYIADEGKACVVVVNKIDLVEDKAALEENLSHQLRFID
                     YAKRVNVSALTGKNIIKIFPHIVAALKSYNDRITTGRLNRWLAQVTDKQPPNVHKGKV
                     LKIYYVTQAITRPPTFQFFVNDINAASESYMRFLENNLREEFELEGTPVRFSRRQHR"
     gene            10308..10598
                     /gene="gatC"
                     /locus_tag="PK717_00740"
     CDS             10308..10598
                     /gene="gatC"
                     /locus_tag="PK717_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOR46232.1"
                     /translation="MSVEKKDLKRIFELSKLDHAKSDGLEKDIERLVESFSQIRNIDL
                     KDCEPMVLPKWCILRYRKDEPLEGIKKSDALSQAPKTDGTFYIVPKIISGEP"
     gene            10595..11974
                     /gene="gatA"
                     /locus_tag="PK717_00745"
     CDS             10595..11974
                     /gene="gatA"
                     /locus_tag="PK717_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOR46233.1"
                     /translation="MNISEARNLLKSKSISPLELVEECEKNWKNKEHDLNAVITPMFE
                     KAKEVAKKAVFDESKPLSCIPYAVKDNICVKGERLTCGSRILGEYRSLFESTATSRLA
                     SAGAIAVCKTNMDEFAMGSSGEYSAFGPTHNPLDKKLVPGGSSSGSSAAVAAGYSIFS
                     LGSDTGGSVRQPAAFCGLSAIRPTYGTVSRYGLVAFTSSIDQIGPMAKNSIDALTVLK
                     AMRGLDEKDSTSVEWLPVELPEKPVIGIPKELFELDIDPEIMALLQECKKKLEGTGAR
                     FIEVSIPNLKHSVATYQLITPSECSANLSRFDGVRYGLQVEDPELGKQYKKTRSDGFG
                     SEVKRRILIGTYALSEGFYDAYYLQACKMRAKIASELYSALSKANAILTPTTPTTAFE
                     VGSIQDPLKMHACDLLTIPQGLAGIPAVTFPFGKDGKGRPIGMQLSGSAFSDDSLAML
                     AGKVEEVEQ"
     gene            11974..>12349
                     /locus_tag="PK717_00750"
     CDS             11974..>12349
                     /locus_tag="PK717_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46234.1"
                     /translation="MMKCCKCKKPSPYPVCETCANEREKKVIEEIRGIDDLVADHKLS
                     MKKVRRSGFAITPKRLWGLLLVLTGIILAFSGNGYLGMLSLGCIGAGLIFLLLSWTET
                     LTVSLVNDGLTSEYRQIITDLAK"
BASE COUNT         3512 a         2853 c         2791 g         3193 t
ORIGIN      
        1 aaggctggtc tgctgttttc tcccagccct gcctgcctgt tgtctcatgc ttgctattgt
       61 gccgggatag ccgaccatta ctgatgcttc gagtcttcct atgtctattc caagctcaag
      121 ggcattggtt gaaacaacgc ccattatctt gccttccctt agtccctttt caatctccct
      181 gcgctcaagg ggcagatatc cacccctgta acccctgatt gaatcaggat taaatccagc
      241 ccttgaagcg tggtccttta ggagcttcaa tacattttcc acggtggtcc tcgccctggc
      301 aaaaacaatg gtctgtatgc ccgactggac gagctgggag gcaaaccgtt cggcctcaaa
      361 aatagaagat ttgcgcagtc caagctgctt gtcgacaacc ggtgggttgt acaccacaaa
      421 atatttgtcg ccgcttggag aaccatcctc ggttattgcc tcaacatcat cctcgatgat
      481 tctggatgca agatcggcag ggtttgcaat tgttgccgaa gacatgatga aagttgggtg
      541 gcttccataa aaagtgcaaa tcctttttaa ccttcttata acatttgcca tgtgtgaacc
      601 aaaaacaccc ctgtactggt ggagttcatc gatgacaaca tatttcaggt tctcaaaaag
      661 gtgcacccat tttgtgtggt gtggcaaaat tcctgcgtgg agcatgtcag ggttcgtaag
      721 gattatggag ccttctactt tgaggatttt tcttttgccc acatcggtgt caccgtcata
      781 agtgtgaaat tttacaccaa gtcctgttgc ttcaactagc tccgaaagcc cggtttgctg
      841 gtcgaatgaa agtgccttgg tcgggaaaag ataaattgct ctggccgctg ggtccttgga
      901 gattgcagac aaaaccggga cattgtagca gagggtcttt ccggaggccg taggagtggc
      961 aactacaaca tttttgccag acaaaacacc ctcaatcgct tctgactggt gcgaatagag
     1021 tttctcaatg ccaatttttc tgaggccagc cgaaacagtt tcgggcacac aaaacggaat
     1081 gtctgaaaaa gtcccttttc ttccctcaaa atgtatcagg gtcatctctt ttccggattc
     1141 attcctataa atctgggaaa caatctcttc tatggacacg gatgaattct aaaacaagca
     1201 aaaccgcgtg tcaacgaaca ggaggattta acttgttttc tctatttact tttaagggtt
     1261 ttgcaataga attagtcact agtgtccaat gatgggcgca aaatacgatg aggtgaatga
     1321 tggtagtctt tgagttcgat gaagaaacgg gaaaagccgt ggagcaaccc aggagaaaaa
     1381 agaagtccag ccagaagtag taaaatctga gtcatctagg ttggaaaaag ctgtcaatga
     1441 ctttgaaatg aagtctgtca aaagaggaca gcttgtaaac gcgacagtgg tgaaggtcac
     1501 gtctgatggt ctcctggttg atgtcggcca gaagtcagag ggatttatcc ccagggcaga
     1561 ggcatctaca agagacgacg tagagcttgc atctacgttc aaaacaggag acgtgattca
     1621 gtgccgtgtt gtcaagattg gcgatgatga tggcatgata atactctcca agaagcgtgc
     1681 cgattttgag cttgtctggg acaggctaaa agaggcaaag gacaacaagg aaatcctcac
     1741 aacggtcgct acaaaggccg tcaagggtgg acttcttgtt gatgtcggcg taccggcttt
     1801 cgtgccgagg tcacagattg accttaaccg caatgccgat ccgacaaagt gggttggcaa
     1861 ggaactcaaa gtcaggatca tagaagttga ccgcccgaca aggcgcgttg tttgttccca
     1921 aaaggaagtc ctggaagaag accgcgaaag aaggcgtgaa gaattcatct ctaacctcaa
     1981 ggttggagaa atctatgaag gcacagttgt tggcctcgta gaatttggcg catttgttga
     2041 cctcggtgag ggtgtggaag gtcttattca cctttcagag ctttcctggg gtggaagaaa
     2101 gaccccacag caggtgctca agaaacgcca gaaagtcaac gtcaaggtca tcaaaatcgc
     2161 aggagacgag ggcaggatca gcctctcgct caggcaggca cagccacatc cgtgggaact
     2221 tgttccatac aagtactcgg ttggcgatgt cgtcgaaggc acagtcacca gactgctgaa
     2281 gtttggcgcc gttgtcgagc ttgaagaggg tgtctcagga ctacttcaca tatcccaggt
     2341 ctcaaaccac agggtcgaga gaattgacac tgaaattaaa gagggacaga cagtccagtg
     2401 caagattctg gaaataaggg tcccagatcg caaaataagg ctttccatca aggcaaccct
     2461 cccagaggag ccaagaccgg agcgccagcc aagaaagcgc tttaacgctt atgaagatga
     2521 agaagagcag tatctcaggg aatttgccca ggaatcggca gacccatcag taaggctcat
     2581 ctcggagaac gacctcgaga gggaaaaccg cgaagtcttc aacgtcaacg agtgatatag
     2641 acacccaaca aataaggaga agttttgaaa tgaaaaagta tttagcagtg atagccattt
     2701 tggcacttgg aatcaccttc atggccgctt gcggttcaaa aaacgaagag actaccctac
     2761 caaccgaaca agaaatgagt aaagtaactg accagtacaa caccgacaat cagacaactg
     2821 accagaccac aacaaccccg actgacaacg caaccgacaa gactggcaca gaaaacccag
     2881 cagaggaaga ggacaaagat atgggaacaa caaataccga tctcggcaag accgtcgttt
     2941 tcgaaaccaa ttacggtgct tttgaaatca gcctctatag tgatgctcca attgcttcaa
     3001 agaacctcat tgataaagca aattccggct tctataacgg actcacattc caccgcatcg
     3061 ttaaaggttt cgtcatccag ggcggcgatc cacttggaaa cggcaccggc ggcgaaacaa
     3121 tgggtcttga tccaaagggt cttcaccaga gcaaaagggg aacaatctcc atggcatctg
     3181 ccgatatcaa aacaaaccag tccgatgcac agttcttcat caacctggta gacaacttcc
     3241 gcctcgacga aatgggattt attgcattcg gcgaagtaac aaagggcatg gaagtcgtgg
     3301 acaaaatcgc agaagtccca tgcaatcctg gtggagacgg tgcaaattcc agcccagtct
     3361 cgccagtact catcaataaa gtctatgtca aataggatag ttttggcctc tctggccacc
     3421 ctcgctctgg tagtttcaat gaccagctgc acgggtgtgt cgctcgaaga gaaacaaaac
     3481 gaagtcccac cagaaaaacc aaaaatcacc ttcagcacaa ctgaaggtga ttttgttatt
     3541 ggggtacttc caaaaaacgc ccccaattca gttaataaat tcttaacctg gtgcaaaggc
     3601 ttcactacgc cagaagggca agtagtcgca tgtatctaca atggtcttca gttttacagg
     3661 gtgatgcctg gcaaattcgt tcagggtggg gaccctttca acaatggcac cggctccgat
     3721 gcattcaaaa taccctttga gaaaactgaa aagaaactca agagaggcgt tgtttgtctg
     3781 gcaaatgatg gtgaaggaaa caacaattgt attttcttca tccttcttac agacactcca
     3841 caattccagg gcaaatatac ggctgtaggt gaagttttgc agggccttga cgtgattgat
     3901 cgcatgagca atgttccaac tcgtccaaat gagcccaaca caccccttcg caacatctca
     3961 ataatcggtg tcaaggtaag tgagtaagat agcactctac tgcttttcag gcactggaaa
     4021 cacaaggctg gttgcccaga aggtcaaaaa gggccttgag ctagaaggtc acgaggttaa
     4081 ttattttgaa accagggaca aaccaaccat cccaatggaa tatgattctt ttgggatatg
     4141 ttttccagtt tatgactggt cacccccatg gcctctcagg gaactcataa aaagctggca
     4201 tggcttacta aatgacaaac catgttttat tgtaatggac tacgccggtg atccagtcaa
     4261 tgcacgcctc aaaatgatgg aagaccttga aaaaaccggc gcaaggattt tccatgcaag
     4321 cgagattgta atgccagaat catggaccgt tgtattgacg ccaaaaaaaa tccaatcaat
     4381 gaacgaatgg ctttcaaaaa atcctcaacc agatccaacc gatttgggca ttgaggttgg
     4441 caagaaatta aaaaatggcg ataccacgcc aacaaaaaaa ccaaaataca aattttccag
     4501 ttacagtttt ttgatacctt tctacacaaa aacatcactc aagatgttct acaggataga
     4561 tcttgacaag ggaaaatgtg tcaagtgcgg catctgtgct gagaaatgtc cgactggtag
     4621 tataaaacta aactcctatc cttcattttc ctatccatgt gcaagttgtt atgcgtgtat
     4681 aaaccactgt ccaacgcaag ccctagaatc atgggcaaca aggggaaaaa taagatacaa
     4741 accaaaacga gttgatctat aaaataaaaa aggcgaagaa atatcctcgc cttttttatt
     4801 tattctggga ttaagtttct tatgctatga cctttatcat ttccccattt ataaggccct
     4861 gtataaaggc cttttgcgaa tccagctgtt caatgtagtc aacgatctgg gaagttataa
     4921 gttctccatg tatcaatgtg ggtatgcctg gcgggtatgg gcagacaaca tcggcagcga
     4981 ccctgccaat tgcctgtttg aggggaacga gttccgattt tgaatagaaa gcttttcttg
     5041 ggagcatttc aagcggcggg atttctggaa ggggtacttt aacacttggc ctgcaatcat
     5101 atttcttgct tgccagcttc tttaatactt caaccagccc cttaacatat tcccactttg
     5161 ttcccacagt caggaagcag aggaaattat ctgcatcaga aagctctatt tcgacattat
     5221 actcattgtt taataccctc tcggcttccc agccagagag gccaagctcg gagacattga
     5281 tggttatctt tgtcctgtca aaaccaacca ctccctgtct acccacaacc tcacttcctg
     5341 ggcatctcaa cccaggaatt ttgttgattt catctcttag ccaatcagcc atttcaagaa
     5401 tttgggtaag tctttcctga ccatggatgg ccatatacct tcttgcagca tcaagcgaac
     5461 tcataaaaac ataggacggt gaggacgatt gcaaaagaac aagtatcatc ttgagtctat
     5521 cggttgccgc tcttgctgtc accacatgca atagcgaact ctgcgtcatc gttggcaatg
     5581 ttttgtgcgc actctgggta acgcaatcaa catcacactg gcaggcggac attggcattt
     5641 caggatggaa catgaagtgt gcaccgtggg cttcatccac aagcactctt aggcctttta
     5701 tcgttgccat ggaagcaagt gtctgtatat cagaagcgag tccaaaatag ttgggtgtgg
     5761 tcataagaac agcttttgcg gatggatggc gcttcatggc attcgaaagt gtcgttggag
     5821 ttatcgaagt atactcgcca atgtcggaat tgtaatcggg catgacataa gcaggttcaa
     5881 gcccaccaag tataagtcca ccgactgtac acctgtggct atttctttgc aaaatgattt
     5941 tttcctcgtt ccaaacagag gacaacatca tggcttgtac gccaacagtt gttccattta
     6001 taaggaactt ggtttccctg acgccaaaga catcagcggc atacttttgc gcttcgccaa
     6061 taacaccact cgacgtgtgg agataatcaa gaccctggac ttctgtaaga tcaaacttgt
     6121 aaagtccttc accaaaccat tctgcgagtg gttctggaat gactccaccc ctcatgtgtc
     6181 ctggcatatg aaaaggcaac ctattttgcc cgatgtactc aatcaccttc tcgacaagcg
     6241 gagctcttgt ctggtcaagt ttatactttc tcacctggtt acccccttag aaacgcaatt
     6301 ggcctaacat aatacgcata atttaaaaca tgtctagtgc aaagtaattt tgcccggtgc
     6361 aaagtctgtt gttacaagtc aaacgtaatt ttacattcat ccaaattcaa gtataaagat
     6421 gtcaaaggag gcaagatgaa actggatcaa aatagtcttt cgaaaacgct ggatgatttt
     6481 aatgaacaca ttttatttgg taaaaacatt cccaaaaaag cggccgaaga agcagcgatc
     6541 tggatagcct caagacaggg gttgccaggc tcttattaca acatgttcgc accaacagca
     6601 aatgactttc ttgaaacaaa actctttact ggtgaaaagg ttgcttcaag ggccgccaca
     6661 gcgcacatac tgagcgaaga ggcgtgcagg acattgatca aactaggcgc caagaatctc
     6721 caggccaaaa aagcccttga aacagccaca gaagacatgc tcggaaggat aaaagacaac
     6781 ccaagtggga agtattgttg cgggacatgt tcggtgtcct tctggagaca ccttcaggcc
     6841 ggtggcctct cattgcagga ggaaaggatt gcaaatggaa tgggggattt aaaaagaagc
     6901 cgcataggaa acagcaaatg gaggttttat ccattttttt atactctcta tacgttgttg
     6961 gatatcgaca tgaaagaggc gctggaggag ataaaatatg cggcaccagc catggagaag
     7021 tcgctgaaat caggaaaagc tgatgagcca tacggcgaaa gaagggccga gatttttaaa
     7081 aagttgttgg caaaaatata gcatgtaatt tttgatacaa aagttggcaa ccaaattaaa
     7141 tggtcgggac ggccagattc gaactggcga cctcttggtc ccgaaccaag cgctctacca
     7201 aactgagcca cgtcccgaca aatttagcat tttactgatt atttggtctt tgtaaagaga
     7261 attttttaat ctttatatat tgtaattcta tgacacaata tagtgctggt tttgcacatt
     7321 tttgccaaaa aaccctcatt ttgtctataa accacactga cggcagccca gaaaataaaa
     7381 aagccctaca agagggcaaa tatggtcggg gcgagtagat tcgaactacc gaccttcagt
     7441 cccccagact gacgcgctaa ccaaactgcg ccacgccccg aacaaataat aatggcctgg
     7501 tgattttaac acaccaggcc atcaagtcaa gtcaaacgct actactcaat cacttctttg
     7561 cgtggtgaac ggtatggact gcggatttga ggtccttgcc tggtttaaag gctgggactt
     7621 tcttctctgg aatgttgagt ggcttgcgtg tcttcgggtt gacgcccttg cgtgccttac
     7681 ggttcctcac atagaagctg ccgaaaccaa caaggagaac tttgtcgcct gtcttcatag
     7741 catccttgat tgattcaagg gtggcgtcga gcattgctga gcaatccttc tttgaaaatt
     7801 tcgttgttga tgcgatcttg tcgatcagtt ctggttttgt cattttgacc tcctatagtt
     7861 ttttattctt ctttcaattc cctcttcatg agccttctaa tggcccttaa gggggtggta
     7921 ctgcccttcg atactgcatc tatttcagat gttatcggta gctcaattcc gtacttcctg
     7981 gccagaagga gtaatccctg cagtgattca agtccttcta tggcctgtcc gtccggtgat
     8041 atttcatctc tggcttcgtc aggtttttta ccttttccga gcagataccc gaagcgcttg
     8101 tttcttgaga gtggcgaaga acatgtggcc accatgtccc caagacaaga caaccccctc
     8161 agggtcgcct cctgggcacc aagcgcctta tacaaccttc ttgcttcctc aaggcctctt
     8221 accactagtg cagctttggt gttgtctcca aaaccaagtc cttcgataac acctgcgcca
     8281 atagcaacta cgttcttcaa ggcaccacca agttcaacgc ccttcacatc tttcgagcga
     8341 tagaccctga aataagtgtt tgaaaacagt tgctggattt gctttgaaag ctcatcactt
     8401 ttcgaagcgc ataccgcagc ggtgggcatc ttctgggcaa cctctctggc aaaatttggg
     8461 cctgaaagaa cagcgatctt gtcactgtcc agattccatt cttttgagag gagttctgac
     8521 atggtgagtg attgttcagg ctcaatacct ttgaccacag aaactacctt agtttgattt
     8581 tcaaggttgc gggcaaactc ccttgcagcc atggctagat atttcccggg tacagcggcc
     8641 accattatat cgataccttt tgaggctgtc aaggggtcgg actcaataac gaccttgtct
     8701 tggagtttat aaccaggaag atacctgata ttttcagcat ttcttctaat ttcatcggcc
     8761 agatcaggag tattcgccca aaggataact tctatcggtt tggaagccaa aactgtggtc
     8821 aaagcggtcc cccaagctcc ggcacccatg acacaaaccc tcatctatgc tgtctccttg
     8881 agaatcttac gggagtacct tcaagctcga attcctctcg taagttgttc tccaaaaatc
     8941 tcatatatga ctcggaagcc gcattaatat cgttaacaaa aaattgaaaa gtcggtggac
     9001 gggttattgc ctgggtcaca taataaattt tcaaaacttt ccctttatgg acgtttgggg
     9061 gttgtttgtc cgtgacctgg gccaaccatc tgttcaatct gccagtggtt atcctatcgt
     9121 tgtaactctt taatgctgca acgatgtgag gaaaaatttt tatgatattt ttaccggtta
     9181 acgcagacac gtttacacgt ttcgcatagt caataaacct aagctggtgc gacaaatttt
     9241 cttcaagtgc tgctttatct tcaaccaggt caattttgtt taccacaaca acacatgctt
     9301 ttccttcgtc tgcaatgtac cttgcaagat gctggtcgtc tgatgtggcg cctactgagc
     9361 catcaatgac caaaatcaca agatcgctcc tgtggatggc ttgttctgac cttttgatgg
     9421 tcacataatc aagctgttcg tcatacttgt agcgtttttt gattcctgct gtgtctacga
     9481 ggatatattt agtgccatca tactcaatga gcgtatctac agcatctctg gttgtccctg
     9541 ggacatcgga gacggttgtt ctcttttcac ccaaaaacgc gttcaggaga gatgatttgc
     9601 caacattcgg ccttcctgct atcgcaattc ttcttggtgg caattcttct tcctgttgct
     9661 cctgttgttc ttcctcgact gccttgccaa tactttcgac tattagatcc aagagttcgg
     9721 caacattcct gccagaggtt gcagacacgg taaaaggatc accaaaacca agctggttaa
     9781 aattgaaatc ggtccagtct ttattgtcag ccttgtttgc agcaagaatg atctttttag
     9841 tggactttct aagcatttta gcgacataaa tgtcttcttt ggttgggcca atctgggagt
     9901 ccaccatgaa gacaatgacg tcggcaatgc caattgccat ctcagattga agggaaacct
     9961 gcctttgtat tgtggaggct tccggtgcta tcccgccagt gtccatgagg gtgaaatcaa
    10021 caccgttcca ggtaaaatcc tccatgatcc tgtcgcgggt cacacccatc gcctgatcag
    10081 taatggtcct cgcctttctg aggattctgt tgaatattgt ggacttgcca accgacggtc
    10141 ttccaatgaa ggccacgatt ggcaggttct tggctcttaa gggttttatc gaggagattt
    10201 cgttttccat ttgttctacc tgaaataagt attgccccaa acgacatttg tcaagtacac
    10261 tggagaattg gcaaatactg gctataattt ccccttcagg aggcaggatg tctgttgaaa
    10321 agaaagacct caaaaggatt tttgagctat caaaactcga ccatgcgaaa tcagatggcc
    10381 ttgaaaaaga cattgaaagg cttgtcgaaa gtttttcaca aatcagaaac atcgacctaa
    10441 aggattgcga gccaatggta ttgccaaagt ggtgcattct cagatacagg aaagacgagc
    10501 cactggaggg catcaaaaaa agcgatgcgc tctcgcaggc accaaaaaca gacgggactt
    10561 tttatattgt tccaaaaatc atatctggtg aaccatgaac atatcagaag ccagaaatct
    10621 tctcaaatca aaatcaattt cgccccttga acttgttgag gaatgcgaaa aaaactggaa
    10681 aaacaaagaa catgatctga atgcagtaat aactcccatg tttgagaagg caaaagaagt
    10741 cgccaaaaaa gctgtttttg acgaatcaaa accactctcc tgcataccct acgccgtaaa
    10801 agacaacatt tgtgttaaag gggaaaggct cacatgtggc tccaggattc ttggcgaata
    10861 caggagtctt tttgaatcga cagcaactag taggcttgca agtgctggtg ccattgccgt
    10921 atgcaagaca aacatggatg aattcgcaat gggatcatct ggtgaatact ctgcctttgg
    10981 accaacccat aatccgctgg acaaaaaact tgttcctggt ggttcttcat ctggctcctc
    11041 tgcggccgtc gccgcaggct actcaatttt ctcacttggt tcagacacag gcgggtcggt
    11101 acgacagcca gcagcattct gtggattgtc tgccataaga ccaacctacg gcacagtgag
    11161 tagatatggc cttgttgctt ttacatcatc gatcgaccag atagggccaa tggcaaaaaa
    11221 ttccattgac gcactcactg ttttgaaggc catgcgcggg ctggacgaga aagactcgac
    11281 aagcgtagaa tggttgccag tggaattgcc ggaaaaacca gtaattggca tccccaaaga
    11341 gctctttgaa ctagacatag accccgaaat catggcattg ctccaggaat gcaagaaaaa
    11401 gcttgaagga accggggcaa gattcatcga ggtttccata ccaaacctca agcattcggt
    11461 ggcaacctac caattgataa caccgtccga atgcagcgcc aacctctcaa gattcgatgg
    11521 tgtaagatat ggacttcagg tcgaagatcc agaacttgga aagcagtaca aaaaaaccag
    11581 atcggatggt tttggatctg aggtcaagag aagaatactg attggcactt atgccctctc
    11641 cgagggtttc tacgatgcct actatctcca ggcatgcaaa atgagggcaa agatagcaag
    11701 tgaactgtat tctgcattgt caaaagcaaa cgccatcctg acaccaacaa cgccaactac
    11761 tgccttcgaa gtaggctcaa tccaggatcc tcttaaaatg cacgcctgtg accttctgac
    11821 aattccacag ggtctggcgg gtattccggc agtaacattc ccatttggca aagatggcaa
    11881 aggaaggcct atcggaatgc aactttcagg atcggcgttc tcagatgatt cgcttgcaat
    11941 gcttgcaggc aaggtcgaag aggtcgaaca ataatgatga agtgttgcaa gtgcaaaaaa
    12001 ccatcacctt atcccgtttg tgagacctgt gcaaacgaaa gagagaagaa ggtcatagaa
    12061 gagataaggg gaatagatga tctcgtggct gaccataaac tctccatgaa aaaggtcagg
    12121 aggtcaggtt ttgcaataac cccgaagagg ctctgggggc ttcttctagt gcttacaggc
    12181 ataatccttg cattcagtgg aaacggctat cttggcatgc taagtttggg gtgtattggt
    12241 gcagggttga tatttctttt gctttcctgg accgaaacac ttacagtctc actcgtcaat
    12301 gacgggttga ccagtgaata cagacaaatc attaccgatc tggcaaaaa
//
LOCUS       DAOSRI010000013        12000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5294_length_12000_cov_4.622436, whole genome shotgun sequence.
ACCESSION   DAOSRI010000013 DAOSRI010000000
VERSION     DAOSRI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_12000_cov_4.622436"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK717_00755"
     CDS             <1..220
                     /locus_tag="PK717_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46235.1"
                     /translation="VAETSGIQTQRTVKDVYDMHDMRIARLVSSASIITVGIPARRFG
                     MFGSVSSVSDAQAAGKLLSKALRKKLVK"
     gene            265..1362
                     /gene="gcvT"
                     /locus_tag="PK717_00760"
     CDS             265..1362
                     /gene="gcvT"
                     /locus_tag="PK717_00760"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOR46236.1"
                     /translation="MKRTPLYEKHVALGAQIVPFAGYEMPIQYTSILDEHKTVRENAG
                     LFDVSHMGEIEIKGPEAVALTDQIMSNNVADMKPGTAEYTVMCKEDGGIIDDLFIHYI
                     SKDHIFLVVNASNVDKDFAWIKSHERPGAQVTNLSDQYAEIALQGPKAEDIIRKVAEF
                     PVWDLPYFHFGYGKVAGREVMVARTGYTGEDGFEIYTSNQDAPHVWDELLRVGKEFGI
                     KPIGLGARDTLRFEVCYWLYGNELDETINPLEAGQKFVVKFEKDFIGKEALLKAEADG
                     IKRKLVCLKMLEEGGIPRSHFKVFDGSTEIGFVTSGGMLPTAGYVGAMAYVPTTYKVG
                     TKVSVEIRGRKLAAEIIKKPFYKGTSGKKRE"
     gene            1404..2222
                     /locus_tag="PK717_00765"
     CDS             1404..2222
                     /locus_tag="PK717_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR46237.1"
                     /translation="MMRDIIQLEKVGRQIRRDIIQMTALAGSGHPGGSLSATDLMAAL
                     YFEIANVDPSNPMKEDRDRIIYSKAHVTPLIYSVLARRGFFDPELLKTFRKLGSPLQG
                     HPHIHCVPGIEMSGGSLGQGISIALGTAIAGKMDKAPWRVYCLMGDGELQEGQVWEAF
                     MAAAHYKADNLTAIIDKNGLEIDGTTKDVMNIDPLEKKLEAFGWHIIECDGHDYGQIL
                     YAFDQAKQIKGKPQAVIAHTVKGKGVSFMENNAEWHGKAPNQVETEKALLELAD"
     gene            2373..5675
                     /locus_tag="PK717_00770"
     CDS             2373..5675
                     /locus_tag="PK717_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46238.1"
                     /translation="MKTKSGFIVLFLIMAIICQQTVVFAEFGPVKVVPGGQPMQTTQR
                     ACRANQPTAFSINFRHTETINFHDWIKVWFPANETLCSIEDACDGIPGISGTLENARF
                     IPCKDYFEKYKDSKESDLGKLYRITNSETADTEFFDCESNQEQKSDCKMIKDPSGLGY
                     WLTGTVMPALPKNDAKRVEKLGNLDHKIRLAGIDCGCSPVYPVIVNIPEERSLKITSS
                     FQIWKGNGPFRNNDDYINLFIQKDVGIISPMSPGKYSVMVATKPEPIPIESEMFVIPC
                     SSISKPVVSFETSDIESASQVKINFSTGEGGALDKGISKIWLEFPEGFSLPKEVLPQN
                     MKINGKPCPKNTSLIATDTQTISVVLPIDINNSSEIEIVFSKLAGIRTPGKKGTYTIN
                     AWTDPEPEKVESEPFLIQTRTRVEVEPNLYMHNAKYLAVFNMDDNQQIRLNQEIMITF
                     PEGTILPATAKQGTVLINEIGYAQSISIENKKVTIKAPFEISKMLKIEFSKEFGIKNP
                     PEGEHELFFTIGGKTNSYGKFTIISKPLVSDLILSDYKAKEVSGFSFKYFPSATGNLS
                     PGDIMTLIFPEEYAIPEKIAPENIKIKGHPSRNVLIQKNEITLTLNDEVDLEDNGTLV
                     EISSGAGITNPERRGTYRITVKTSKDEKTESNEYSTKEAKLLTQLVFEDPSEPDGLEY
                     DGWKWFKKPPKVKILSWNPDATVYFWFDNNEAHKTSLDEYPNIDFGEYVQLQSTFSYC
                     SELDDEKEEAKSVKLWVDNILPKIWVQSPEKNILITNKKTITIVGNRAPGELRVYGDR
                     KYTLDGVSINGETILQPEIVEKNLLNSAKMEFEKQILLTEGDNKIKIRGFDLAGNEKI
                     ITKIVTLDTTSPEIEIVTPNPKKPQKIGESIAIQIKTETGALVYINGQLAEKIKELPW
                     GIAVFEGNWNVVKGENKIEINSTDMAGNANTIFLTFEGSLYESIIEFWIGKTEFYVNG
                     EKMPNPSSAPTCSSPPLPKDFKGTAFLPIQYLLTNALYTSASYDGDTNKITITQSSEN
                     EIITVIELGIGEKQAKVNGVEVWIDKNKILYPIIISGSVFFPLRFMAENLNCDVKYDP
                     KTKKITLAYPKQ"
     gene            complement(5688..9254)
                     /locus_tag="PK717_00775"
     CDS             complement(5688..9254)
                     /locus_tag="PK717_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR46239.1"
                     /translation="MEERLIFGRYRLLRKIGSGGMGEVFAALDLQSNEDVIIKISHFK
                     DDDWDNVEKNRFLSEASILASLDHPGIPKVWDCCREGEDYYYIMEYVKGTPLSKLHNV
                     GIEQALGIFYDVAGILSHIHSRSVVHRDIKPGNLIVLDSPDIKTGIRVKLIDFGLAKN
                     VVNGRITSTGMVVGTFNYMAPEQLMGGAIDMRADLYSFGATMYNTLTESLPFGSPDPK
                     KIAYQLINASCDPPSHFNGDIPEELDNVILCLLRKDPNERIQSASELLSLMQSITTDE
                     KSNKPAGLVLCEPNMVGREFEMQYLIDSWNSSQTQSVTVCVAGPFGIGKTRLVDEFAS
                     IVQLSGKMLLRARGHGSTNSLPMDGLKQLAFHLAHYNLDQYSDLIEQNSKELGFFSQQ
                     LCKSKRKSAQSFPNQSACSFAFLKFLKAISFEAPIVIILEDIEHLDKETLDVCFELSS
                     NPELKIIVVFTCLEPTFRSNAAIGLKLHRMSDLRFIDLKNLKKSNVKMMMESMLRGHE
                     VPNDFLNDLWERFGGVPYLLINNIRTFVSQKMLALDSGRLVYKRPISFEDVSGSILSQ
                     HLSSLSQISIKILEFACLFEEPFEMEIVMKSLGLTRRQCVNAFYELISSSLLKQRNGF
                     YKSTYEFAYPSMKETVKSRIPDTEKHETHKLIAQILEFSDEVTDEIACQIAKHYKMAG
                     EIDKAVIWAEECAKRLIALGSQTYSEWIDYIHSVGVSESNPVYDVKAKRVLSDIFYAK
                     GDFEKAKALFLEALGQAKKIGVNTEYSLILIHYLKFLFEIGEYSELQKIGSKEAKTPA
                     LMGLPSERFVVFEYLALSLKENSNFSEAEDIAKEALYIAQCSLPDKVAEGKAVLFSCL
                     LGDMKMDDASEMAKEIESEVAELNPDVAERHAIELALFEYNMGNYGKTIEMLSNMDKG
                     LLTFRDKTKSAILKSKSFYALGKFDEAIESVNKLSIPDGAEVSRVELEKLVYKSLFIT
                     LAHGWEKSGPRTERLIGLAEFQECEACKILPYEVYCYWLSKQKRFDDAMEYFAKAWTI
                     SCNLGIEEYMLDCAYWGVSILSYKKIIAKYPSIANLITDEEGLFGGYAQKYEHAVVAG
                     YVYLYRAEKDFREFDHAIRWFVHARELALQKGNKIYMALSAFNLGKAYCERNNSKHKK
                     PYDAEDAEKFFFEAEFVFSAMNAPATVDVVREYQEKHLKRSN"
     gene            9383..9811
                     /gene="rpsF"
                     /locus_tag="PK717_00780"
     CDS             9383..9811
                     /gene="rpsF"
                     /locus_tag="PK717_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR46240.1"
                     /translation="MKNYEQMFVLNPALDEEAEKALAEKLATIVTNDGGEVLDLKKWG
                     KRRLAYEIKGQTEGSYWVLTFKAPPQVPLELRRIVKITEGFLRDILVDLTDSIKAQKN
                     REVHMKAAEARRAAKALARDREREQKQETEQQVEEPPKEA"
     gene            9811..10254
                     /gene="ssb"
                     /locus_tag="PK717_00785"
     CDS             9811..10254
                     /gene="ssb"
                     /locus_tag="PK717_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR46241.1"
                     /translation="MYNKVLLIGNLTADPEIRYTPSGAPVTTIRLACTEKYRSKSSTE
                     LKEETLFINCIVWGNQATSAHQYLKKGSKCFVEGKLVIREYEKDGIRRWVTEVRVQNL
                     KFLGSPTKGTSFENKGQPSQQQHAAEAEEDEFMPPDFNDEDIPFS"
     gene            10282..10545
                     /gene="rpsR"
                     /locus_tag="PK717_00790"
     CDS             10282..10545
                     /gene="rpsR"
                     /locus_tag="PK717_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOR46242.1"
                     /translation="MMDDDRKGRRMGKGRKFSKPRRKVCIYCADHNDYVDYKDVARLQ
                     KYVTDTGKILPRRATGMCAKHQRRLCTAIKRAREIALLPFSAR"
     gene            10552..11010
                     /gene="rplI"
                     /locus_tag="PK717_00795"
     CDS             10552..11010
                     /gene="rplI"
                     /locus_tag="PK717_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR46243.1"
                     /translation="MKTKVILLADVPGVGKKGTVAEVASGYARNYLVKNSLAQIATTS
                     SISNLAYNVEQADKKSAKAKARAEEIKQKIEEKTITISAKAGPSGTLFGSITADMISK
                     AMKRDLNIVMDKKSVDLDEPIKSTGIHTIPVKLHHEVVAELKLEIVPQSK"
     gene            complement(10998..11975)
                     /gene="mtnA"
                     /locus_tag="PK717_00800"
     CDS             complement(10998..11975)
                     /gene="mtnA"
                     /locus_tag="PK717_00800"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOR46244.1"
                     /translation="MPRPLVFEDSILKILDQRALPGKIVYVNVDNWEEVALAIKNMMV
                     RGAPMIGIAGAYGMALAVMKDPGNIEKAAIELKKARPTAVNLPWAVDRILGIARSDLT
                     KAIEEAKKIEVEDHEKSFLIAKNASTLIKPGMRILTHCNSGSLAAGGFGTALGCIVWA
                     KLIDGKEVSVFNTETRPYLQGSRLTSFELVSAGVPDTLIPDMAASLLMSKRMVDLIIV
                     GADRIARNGDVANKIGTLQLAISAHQYDIPFYVAAPRSTFDLTIESGKEIPIEERPAE
                     ELVVFNGIKMAPDGISVFNPAFDVAPFDLVTGYITESGIIGAWKIPYLD"
BASE COUNT         3817 a         2626 c         2497 g         3060 t
ORIGIN      
        1 agtggcagag acttccggaa tccagaccca acggaccgtg aaagatgtct acgacatgca
       61 tgatatgcgc atcgcacgac ttgtctcctc agcatcaatc ataaccgttg gtataccagc
      121 tagaaggttt gggatgtttg gctcggtttc ttcagtttct gatgcacagg ctgcaggaaa
      181 actgctctcc aaagctttgc gcaaaaaact cgtcaaatag gttgaaattt tccagcccac
      241 aactagactc gcttcggagg aaaaatgaaa agaacaccgc tttatgaaaa acatgtagca
      301 ctaggggcac agattgtccc attcgcaggt tacgagatgc cgatccagta cacgtcaatt
      361 cttgacgaac acaaaactgt aagggaaaat gcaggcttgt ttgatgtatc gcacatgggc
      421 gagattgaga tcaaggggcc cgaagctgtt gccctgactg accagatcat gtcaaacaat
      481 gtcgctgata tgaaacctgg cacagcggaa tacactgtca tgtgcaagga agatggtgga
      541 atcattgatg acctgtttat ccactacatc agcaaagacc acatattctt ggtagtcaac
      601 gcatccaatg tcgacaagga ttttgcctgg ataaagtccc acgaacgccc gggagcgcaa
      661 gtgacaaacc tttccgacca gtatgccgaa atcgccctcc agggtccaaa agcagaagac
      721 atcatcagaa aagttgctga attcccggta tgggacctcc cgtacttcca ttttgggtat
      781 ggaaaagtgg caggcaggga agtcatggtg gcaagaactg gctatactgg cgaagatggg
      841 tttgaaatct atacaagcaa ccaggacgcc ccgcatgtct gggacgagct cctgagggtt
      901 ggcaaagaat ttggaattaa accaataggc cttggggcaa gagataccct aaggttcgaa
      961 gtttgctatt ggctctatgg caatgagctt gacgagacaa tcaatcctct cgaggctgga
     1021 cagaaatttg ttgtaaaatt cgaaaaggac ttcattggaa aagaagctct tctaaaggct
     1081 gaagctgatg gaatcaagag aaaactcgtt tgtttgaaaa tgcttgaaga gggcgggatt
     1141 ccaaggagcc acttcaaggt gtttgatgga agcacggaaa tcgggtttgt gacatccggt
     1201 ggaatgctcc caactgcagg ttatgtgggt gcaatggcct atgttccaac aacatacaag
     1261 gtggggacaa aggtaagcgt tgaaataagg ggaagaaaac tggcagccga aatcatcaaa
     1321 aaaccattct acaagggaac ttctggcaaa aagagggaat aaaaagccca aaaagcaata
     1381 caaaataaaa caaacggagg aaagtgatgc gcgacatcat ccagctcgag aaggttggaa
     1441 gacagataag aagggacatc atccagatga ctgcactggc agggtccggc catccaggcg
     1501 gatccctctc tgccactgac ctgatggcag cactttactt cgagatagca aacgtggacc
     1561 catccaaccc catgaaagaa gacagggaca ggattatata ctccaaggct cacgtgaccc
     1621 cgctcattta ctcggtactt gcaaggaggg gattctttga tcccgaactc ctcaagacct
     1681 tcagaaaact tggatcaccc cttcagggcc acccacatat acactgcgtt cctgggatag
     1741 aaatgtccgg tggatcactc ggccagggga tttccatagc cctcggcaca gcaatcgccg
     1801 gaaaaatgga caaggcacct tggagagtct actgcctgat gggtgatggc gaattgcaag
     1861 aaggccaggt ttgggaagcg ttcatggccg cggcccacta caaggcagac aacctgactg
     1921 caatcattga caagaacggc ctggagattg acggcacaac caaggatgtc atgaacattg
     1981 acccgctcga aaaaaagctg gaggccttcg gatggcacat cattgagtgc gatggccacg
     2041 attatggcca gatactctac gcttttgacc aggcaaaaca gataaaagga aagccgcagg
     2101 ccgtcattgc tcacacagta aaaggcaagg gtgtttcctt catggaaaac aacgccgagt
     2161 ggcacggcaa agccccaaac caggtcgaaa ccgaaaaggc gcttctggag ctggcagact
     2221 aataaattgg catttcctca aatatacaaa aacccctggt ttgtccaggg gtttttgttg
     2281 tttaccccaa cagaaccagg ttgtcttctt tttagtatct gctaaaataa ttccagatat
     2341 cattcatgca tgtgcaatgg ggaggtcatg cgatgaagac aaaatcaggt tttattgtct
     2401 tgtttctcat catggcaatc atttgccaac aaaccgtagt gtttgcggaa tttgggcctg
     2461 taaaagtggt gccagggggc caaccaatgc agacaacgca aagggcctgc agggcaaacc
     2521 agccaacagc tttcagcata aatttcaggc atactgaaac aatcaatttt cacgactgga
     2581 tcaaagtctg gttccccgca aatgaaacat tgtgtagcat tgaagatgcc tgcgatggga
     2641 taccaggaat tagcggcacg ttagaaaacg ccagatttat tccatgcaaa gattattttg
     2701 aaaaatataa ggatagtaaa gaatctgatc ttggcaagct ctataggata acaaacagcg
     2761 aaactgctga tacagaattt ttcgattgcg agagtaacca ggaacagaaa tccgattgta
     2821 agatgataaa agacccatca ggattaggct attggctgac tggaacggta atgccagcgc
     2881 ttccaaaaaa cgacgcaaaa agggtggaaa aactaggaaa cctagatcat aaaataagat
     2941 tggccggcat tgattgcggt tgtagccctg tctatcctgt tattgtaaac atacctgaag
     3001 agcgctcact caaaataaca agtagctttc agatttggaa ggggaatggc ccattcagaa
     3061 acaacgatga ttacatcaat ctttttatcc aaaaggatgt cgggataatt tcgccaatga
     3121 gccctggcaa atactctgta atggttgcca caaaaccaga gccgatacct attgaatccg
     3181 aaatgtttgt tattccatgc tcatcaatat caaaaccggt ggtttcattt gagacaagtg
     3241 atatagaaag tgcttcacag gtcaaaatca atttcagtac tggcgaaggt ggggcgctgg
     3301 acaaaggcat ttcaaaaata tggctggaat ttccagaagg gttttcgctc cccaaagaag
     3361 tattgcccca aaatatgaaa ataaatggga aaccatgtcc taaaaacacg tccttgattg
     3421 cgactgacac tcaaacaatc tcggttgttt tgccaataga tataaacaac tcatcagaaa
     3481 tcgaaatcgt cttttcaaaa ctggcaggca taaggacacc tggcaaaaaa ggcacctata
     3541 caatcaacgc ctggacagac cccgaaccag aaaaagtcga atcggaacct tttttaattc
     3601 aaaccagaac aagggtcgaa gtagagccaa acctgtacat gcataatgcc aagtaccttg
     3661 cagtatttaa catggatgac aatcagcaga tacgcctgaa tcaggaaata atgatcactt
     3721 ttccagaagg aacaatactt ccggcaactg caaagcaggg aacagttttg atcaacgaaa
     3781 tagggtatgc acaatcaata tccatagaga acaaaaaagt cacaataaaa gctccttttg
     3841 aaatctcaaa aatgctcaaa atcgagtttt caaaagagtt tgggataaaa aaccctccag
     3901 aaggtgagca tgagctgttt tttacgattg gcggcaaaac aaattcttat ggcaaattta
     3961 ccatcatctc aaaaccattg gtctcagatt tgattctttc agactacaag gccaaagaag
     4021 tatcagggtt ttcatttaaa tattttcctt cagcaacagg caatctttca ccaggagata
     4081 tcatgaccct gatttttcct gaggaatatg cgattccaga aaaaatagca cctgaaaaca
     4141 taaagattaa aggtcatcca tccagaaatg tccttataca aaaaaatgag atcactctga
     4201 ctctgaatga cgaagtagac ctggaagaca atggcacatt agtcgagatc tcaagtggcg
     4261 ctggaataac aaaccccgag aggcgaggta cttacaggat aactgtaaaa acaagcaagg
     4321 atgaaaagac tgaatctaat gagtattcga ccaaagaagc aaagttgctt acccagcttg
     4381 tttttgaaga cccatctgaa ccggatggcc ttgaatatga tgggtggaaa tggttcaaga
     4441 aaccgccaaa agtaaaaatc ctttcatgga atcccgatgc tactgtatat ttctggtttg
     4501 ataacaacga agcccataaa acttcacttg atgaatatcc aaacatagat tttggagaat
     4561 atgtacaact ccagtcaaca ttttcttatt gctcagagct cgatgatgag aaagaggagg
     4621 ccaaatctgt taaattatgg gttgacaata ttcttccaaa gatttgggta caaagtcctg
     4681 agaaaaatat tctgattaca aataaaaaga caataacaat agtcgggaat cgcgcaccag
     4741 gagaattaag agtatacgga gaccggaagt acacactcga tggtgtttca attaatggcg
     4801 aaacaatcct ccagccagaa attgtagaaa aaaacttgtt aaattctgca aaaatggaat
     4861 ttgaaaaaca aattctgcta acagagggag acaataaaat aaaaataaga ggttttgatt
     4921 tggctggcaa cgagaaaata attaccaaaa tagtcactct ggatactacc tcacctgaaa
     4981 tagaaattgt gacccctaat cccaaaaaac cacagaaaat tggtgaaagt attgccattc
     5041 agatcaaaac cgagactggc gccctagttt acatcaatgg gcaattggcg gaaaaaataa
     5101 aggaacttcc ctgggggata gctgtttttg agggaaactg gaatgttgtg aaaggcgaga
     5161 acaagattga aataaactcc acagacatgg caggcaatgc caatacaata tttctcactt
     5221 tcgagggaag cctctacgaa agtattattg aattctggat tggaaaaacc gaattttacg
     5281 taaatggtga gaaaatgcca aatccttctt cagctccaac ttgctcttct ccgcccttgc
     5341 caaaagattt caagggaacc gcatttttac caattcaata tctgttaact aacgctcttt
     5401 ataccagtgc ttcctatgat ggagacacaa ataaaattac gatcacacaa tctagcgaga
     5461 acgaaattat cacagttatt gagctgggca taggggaaaa acaagccaaa gttaatgggg
     5521 tggaagtatg gattgacaag aataagatcc tataccccat aatcatttca ggaagtgtct
     5581 ttttccctct cagatttatg gccgagaact tgaattgcga tgttaagtat gatccaaaaa
     5641 ccaagaaaat tactctggct tatccaaaac aataaacttt tcgagcatca atttgagcgt
     5701 ttcaggtgct tttcctgata ctccctgaca acatccacgg tggcaggggc attcatggct
     5761 gaaaaaacaa actctgcctc aaaaaagaac ttctctgcgt cctcagcatc atatggtttt
     5821 ttgtgttttg agttgttcct ttcacaatag gctttgccaa gattgaatgc tgataaagcc
     5881 atgtaaattt tgtttccctt ttgaagcgcg agttccctgg catgaacaaa ccatctgatt
     5941 gcatgatcaa attccctgaa atctttttca gccctgtaaa gataaacata acccgcaaca
     6001 actgcatgct catatttttg ggcgtagcca ccaaaaagtc cctcctcatc agtaatcaaa
     6061 ttggctatag aaggatattt tgcaatgatt tttttgtagg aaaggattga aacgccccaa
     6121 tatgcacaat caagcatata ttcttcaatg cccaaattac atgaaatagt ccaggccttc
     6181 gcaaaatact ccattgcgtc atcaaacctt ttctgttttg aaagccaata acaataaacc
     6241 tcgtatggca atattttgca tgcctcacat tcctggaact cagcaagtcc tatcaagcgc
     6301 tccgttctgg ggccagattt ttcccagcca tgcgccaatg tgataaaaag agatttgtat
     6361 acaagcttct ccaattcaac acgactcact tcagcaccat ctggaatgct taatttatta
     6421 acgctttcaa tcgcttcgtc gaatttgcca agagcataaa atgattttga tttaagtatg
     6481 gcagatttgg ttttgtcccg gaatgtaagc aagcccttgt ccatattact caacatttca
     6541 attgtcttac cataattgcc catgttgtac tcgaacaatg caagctcaat cgcatgcctc
     6601 tccgcaacat ccgggttcaa ttcggcaact tctgactcaa tttcttttgc catttcagat
     6661 gcatcatcca tcttcatgtc tccaagaagg catgaaaaaa gaacggcttt tccttctgca
     6721 actttgtctg gcaaggagca ttgagcgata tagagcgcct ctttcgcaat gtcttcggct
     6781 tctgaaaagt ttgaattctc tttcagggag agcgccaagt attcaaaaac cacaaatctt
     6841 tctgatggta gacccataag agctggcgtt ttggcttcct tagagccgat tttctgtaat
     6901 tcactgtatt caccaatctc aaaaagaaac ttgagataat gaatcaatat taatgagtat
     6961 tccgtattta cccctatttt ttttgcctga ccaagcgctt ccaaaaataa agcttttgcc
     7021 ttctcaaaat ccccttttgc ataaaaaata tcagagagga ccctctttgc cttaacatca
     7081 taaacaggat tggattcgct cactcccacg ctatgtatat aatcaatcca ttcggaatag
     7141 gtttgggagc caagtgcaat caatcttttt gcacactctt cagcccatat taccgctttg
     7201 tctatttctc cggccatttt gtagtgtttt gctatctggc aggcaatttc atcagttact
     7261 tcatcgctaa attctaggat ctgcgcaatc agtttgtgtg tttcgtgctt ttctgtatct
     7321 ggtatcctag attttactgt ctctttcata gatggataag caaactcata cgtactcttg
     7381 taaaaaccat tcctttgctt taaaagtgat gacgatataa gttcgtaaaa agcattcacg
     7441 cattgacgtc ttgtgagacc aagcgatttc ataacaattt ccatttcaaa tggttcttcg
     7501 aaaaggcacg caaactcaag gatttttatg gatatttggg agagtgagga aaggtgttgc
     7561 gataaaattg agccggatac atcttcaaag gaaataggcc ttttataaac aagccttcca
     7621 ctatcaagcg caagcatttt ttgtgaaaca aacgttctta tgttgtttat caatagatag
     7681 ggaacaccac cgaatctctc ccagaggtcg tttaagaaat catttggcac ttcatgacct
     7741 cttagcattg attccatcat catttttaca ttagatttct taaggttttt gagatcaata
     7801 aacctcaaat cgctcattct atgcaatttc aatccaatag ctgcattgga tctaaatgtt
     7861 ggttcgagac acgtaaaaac gactattatc ttcaattctg gattagagga aagttcaaaa
     7921 catacatcaa gggtttcctt gtcaagatgc tctatgtcct caaggatgat aacaatgggc
     7981 gcttcaaacg atattgcctt caaaaatttc aaaaaagcga aagagcatgc gctctgattt
     8041 ggaaaagatt gggcagattt tctttttgat ttacacaatt gttggctgaa aaacccgagc
     8101 tcttttgaat tttgctctat taaatcagaa tattggtcca gattatagtg tgcaagatga
     8161 aaagcaagct gtttgagacc atccatcggt aatgagtttg tcgaaccatg tccccttgcc
     8221 ctcaaaagca ttttccctga taactgcact atggaagcaa attcatcaac aagtctggtt
     8281 tttccaattc caaatggccc agcaacacag actgttactg attgagtttg tgagctgttc
     8341 caagaatcaa taaggtactg catctcaaac tcacgaccca ccatattggg ttcacaaaga
     8401 accagcccag caggtttatt actcttttca tctgtggtga ttgactgcat taatgatagt
     8461 aattctgaag cgctttgtat cctttcgttt gggtcttttc tcaagagaca aagaataacg
     8521 ttgtcgagct cttccgggat atcaccatta aaatgtgatg gagggtcgca agaagcattt
     8581 atcagttgat aggcaatttt tttcgggtct ggagagccaa agggtaggga ttccgtcaaa
     8641 gtgttataca tcgttgcacc aaaagaatat aaatccgccc tcatatcaat agctccaccc
     8701 atcaattgtt caggggccat atagttaaag gtaccaacca ccatgcccgt actcgttatg
     8761 cgcccattta caacattttt tgccaaacca aaatcaatga gttttactct tattccggtt
     8821 ttgatatccg gggagtcgag cacaataaga ttacctggtt taatgtctct gtgcacaacg
     8881 gacctcgaat ggatgtgcga aagtatcccg gcaacatcat aaaaaatacc caacgcctgc
     8941 tctatgccga cgttatgaag ttttgaaagg ggagttcctt tcacatactc cattatgtag
     9001 taataatctt cgccttctct acagcaatcc catactttcg ggatacctgg atgatcaagg
     9061 gaggccaaga tggaagcttc tgaaagaaat ctgtttttct caacattgtc ccagtcatca
     9121 tctttaaaat gtgaaatttt aataatcaca tcttcattac tctgaagatc cagcgcagcg
     9181 aagacttcac ccattcctcc gcttccaatt tttctaagta gtctatagcg accaaaaatc
     9241 agccgttctt ccattatgtc taaattctaa aacaacaatg aaaccagtca atcttgacat
     9301 atcaggaacc taacctaaca tttatttttt agttgcacct tctggtcaat aaaaattgac
     9361 ccacaagccc aaaggaggaa ttgtgaagaa ttacgagcag atgtttgttt tgaatccagc
     9421 tctcgacgaa gaagcagaaa aagctctcgc agagaaactc gccacgatcg taacaaatga
     9481 tggtggtgaa gtgctggacc tcaagaaatg gggaaaaaga aggctggcct atgagattaa
     9541 aggccagact gaaggttcct actgggtcct cacattcaag gccccacccc aggtaccgct
     9601 tgagctgaga aggatcgtca aaatcactga aggtttcctc agagacatcc ttgttgacct
     9661 tactgactca atcaaggccc agaaaaatcg cgaagtgcac atgaaggccg ccgaagcacg
     9721 cagggcagcc aaggcactcg caagggacag agaaagagag cagaaacagg aaaccgaaca
     9781 gcaagttgaa gaacccccca aggaggcctg atgtacaata aggttctgct tataggcaac
     9841 cttactgccg acccagaaat ccgttacact ccctcaggtg ccccggtaac aacaatcagg
     9901 ctcgcctgta cagaaaagta caggtcaaaa agctctacgg agctcaagga agaaacgctc
     9961 ttcataaact gcattgtgtg gggaaatcaa gccacatcag cacaccagta cctcaaaaaa
    10021 ggcagcaaat gctttgtcga agggaaactg gtcatcaggg aatatgaaaa agacggaatc
    10081 aggcgttggg tcacagaagt aagggtgcag aaccttaaat tcctcggatc accaacaaaa
    10141 ggcacatcat ttgaaaacaa gggacaacca tcccagcaac aacatgcggc cgaggccgag
    10201 gaagacgaat tcatgccgcc agatttcaat gacgaagaca ttccgttttc atgaagagag
    10261 gagaactgaa aaatggataa aatgatggat gacgatcgca aaggcagaag aatgggaaag
    10321 ggacgcaagt tcagcaaacc cagaagaaaa gtctgcatct actgcgctga ccataatgac
    10381 tatgttgact ataaggatgt ggccagactt caaaaatatg ttaccgacac tggtaaaatc
    10441 cttccaaggc gtgccacagg catgtgcgca aaacaccaga ggagactctg cacggcaata
    10501 aagagggcaa gagaaatagc ccttctacca ttttctgcga ggtagcttcg gatgaaaaca
    10561 aaagtcatac ttcttgcaga tgtaccaggt gttggtaaaa aaggtacagt agcagaggtt
    10621 gcatcaggtt acgcaagaaa ctaccttgtt aaaaacagtc ttgcacagat agcaaccaca
    10681 tcttcaattt ccaaccttgc atacaatgtt gagcaagcag acaaaaaatc tgcaaaagcc
    10741 aaagcaaggg cagaagagat aaagcagaag attgaggaaa agacaataac aatttcggca
    10801 aaagcgggcc caagtggaac attattcggt tcaataaccg cagacatgat ctcaaaagcc
    10861 atgaagcgcg acctcaatat cgtaatggac aaaaaatccg tagatcttga tgaaccaatc
    10921 aaaagcactg gaatccatac aattccggtg aagctccatc acgaagttgt cgccgagctg
    10981 aagctcgaaa tagtacctca gtccaagtaa ggaatcttcc aggcgccgat gatgccagat
    11041 tcagttatat acccggtcac aagatcaaag ggtgcaacat caaaggccgg gttaaaaact
    11101 gaaatgccgt ccggcgccat ttttattcca ttaaagacaa ccagctcttc agctggtctt
    11161 tcttctatcg gtatttcttt cccactctca atcgtcagat caaacgtgga acgcggcgca
    11221 gccacataga acggtatgtc atattggtgg gcggaaatcg caagttgtaa agttccaatt
    11281 ttatttgcca catcaccatt tcttgcaatc ctgtctgctc caacaattat caaatcaacc
    11341 attctcttgc tcataagaag agatgctgcc atatctggta tcagtgtgtc aggaacacca
    11401 gcactgacaa gctcaaaact tgtgagtctt gaaccctgga gatatggcct ggtttcagta
    11461 ttaaaaacag atacttcttt gccatcgatc agcttcgccc atacgataca acccagtgcc
    11521 gtaccaaatc ctcctgcggc aagcgacccg gaattgcaat gggtgagaat tctcattcca
    11581 ggtttgatga gcgttgaggc attcttggca atcagaaagc tcttctcgtg gtcctcaact
    11641 tcaattttct tggcttcctc aattgctttt gtaaggtcgc ttcttgcaat tcccaatatt
    11701 ctatcaactg cccacgggag atttactgca gttggtcttg ctttttttag ttcaatggcg
    11761 gctttttcaa tgttccctgg gtctttcatg actgccagtg ccattccata ggcacctgct
    11821 atccctatca tgggagcacc cctgaccatc atatttttta tcgcaagtgc aacttcttcc
    11881 caattatcaa cgttaacgta gacaattttg ccaggcaaag cgcgctggtc caatattttc
    11941 aagatactgt cttcaaagac caacggcctt ggaatttttg actgagcaag aagccatgaa
//
LOCUS       DAOSRI010000014        11892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5356_length_11892_cov_3.838557, whole genome shotgun sequence.
ACCESSION   DAOSRI010000014 DAOSRI010000000
VERSION     DAOSRI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_11892_cov_3.838557"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK717_00805"
     CDS             complement(<1..408)
                     /locus_tag="PK717_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46245.1"
                     /translation="MKKHLSCLLLSVIFASSLLVLQACGGPKGGFVDLKIDDPDALVS
                     IDDAKEIPYKELSLPIDLEPVLHKFTVKYSKTGDVVTKFINVSYKETSVLEFKQGEPV
                     EITVNCPLPCKVRIGGQEAGDTRQSNKFNTMAGK"
     gene            complement(410..1150)
                     /locus_tag="PK717_00810"
     CDS             complement(410..1150)
                     /locus_tag="PK717_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR46246.1"
                     /translation="MSLSFSQISTYLECPYRYYLSYIIKLPGLPKPYFSFGSSIHSAL
                     EKLHKPRLIPEKSSLSQLVAWYEESWISDGYADIETEAKAKEEGKGLLVSYFEKYGQS
                     IVPAVDVEKRFKLVMDEITINGVIDRIDRGEDDKLRIIDYKTGNYIPIELGPAEKLQL
                     TIYTMAVGSIYQNKVEKATYLYLKSSVEMSFVPSDDDLASAINTIETVAGQIKEGIFP
                     RCKNKFCPWCDYYQSCNKTSVIHVKEGF"
     gene            complement(1147..1743)
                     /gene="coaE"
                     /locus_tag="PK717_00815"
     CDS             complement(1147..1743)
                     /gene="coaE"
                     /locus_tag="PK717_00815"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOR46247.1"
                     /translation="MAYILGITGGMSSGKTTASKCLQGERTYVIDVDELARPLHQPGC
                     EVYNQIVEKFGKSVCNLDGSINNKRLARVAFATITRTKMLNEIFKEPLTNAIKLEIMQ
                     GRAQRASLIIIVAAILFEQEWDKLCHGVLNISAPESLRLERAMRRGMTERDAQRRMAA
                     QLTEKDRMALAQWTVYTQPTKEELCKKINKLAEENKWL"
     gene            complement(1743..4187)
                     /gene="polA"
                     /locus_tag="PK717_00820"
     CDS             complement(1743..4187)
                     /gene="polA"
                     /locus_tag="PK717_00820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR46248.1"
                     /translation="MAKVLLLDGMSLLFRAYHAMPKMSSKDGTPTGALYGFLRLFYNI
                     VDKEHPDYVAVCLDRKEKTFREEADSSYKANRPTPDEDMIAQIVMFPKLLTDMGIPSL
                     SQAGFEADDVIATVAKKEKENGNQVIIITGDKDVLQTLDDGIKAYINKKGMSEIDEYT
                     PASLQEKTGMTPSQFLFFKALKGDPSDNYQGVPGIGEKTAQKIASEASSVEDVEKNPK
                     VAQNIDAFKHSLMLASIRYDVPLSYKLNDLKMQPDPEKAVSFLRNFDFRTLVSRFAKQ
                     EKTTVRKVSSNEAASLIDGPFALCWEGCLQVWNSNNVLEVDVGSGLFQKTDQALKDLQ
                     KPLLSKHEKVVTDLKELLRTVDVEDPVFDVQLAAWIDEPGRSSYSIESLITAYLDDSP
                     PPGLAIHKIAERVRTNLDEKGQIGLLIDVEQPVSRVLANMETRGIKIDREALRELSLQ
                     LEKGITEKSSQVYEIANTTFNILSPKQLGDVLFQKMGLEPLKKTKTGFSTSQEVLEQL
                     KPASPAIGLILEVRELSKLKSTYVDALPTYCDNQSRVHTTYIQTGTATGRLSSTNPNL
                     QNIPIRSDWGGKIRRCFVTEQGKTFVSADYSQIELRLLAHLSGDEELVKAFNSGEDIH
                     THTARVVFGVSDVTPAQRRQAKIINFGVLYGMSAHRLSNEFGVSFKEADNFIKEYFER
                     FSGVSKYIKEVVEQGRKKGYTETILGRRRYVPDLLSKDFQVRSAGERAAVNSPIQGSA
                     ADIIKLAMIRLEQKLEKTRSRLLLQIHDELVLECPDDETDEMVHLIKEVMENVCESRV
                     PFTCEVHSGKNLLEAK"
     gene            complement(4187..5599)
                     /locus_tag="PK717_00825"
     CDS             complement(4187..5599)
                     /locus_tag="PK717_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46249.1"
                     /translation="MSVKDGFKTAWKWFADATWLQYILVFLGIPALVRWIFQPVVVSV
                     VTKMYNLNNLYGQSLEYTHYARGMADFYGRWMFNVIFLIFLAFFLIYLILKERPNLNL
                     WGLHWNRILPGVLFFAVMWAIIYFIFVPIGSLFTGGDTFYLGIPSPPGTSNYDFHSFS
                     GFGMLLQDMFMTKSLWAFAGPESQTVVPGNWTMGILDFLRTWVLNGPILMFLVFGFFF
                     NKLLTMFSSNDITDNSNHSGSRVFSSLFFASLSVPLLFALYRKVDLVLSSPTIEVSGA
                     TEAATQYGASQGNLIWLLCFWLLIAVLFNLSFLWAYIEKRKKPLGDWGISLSKWLPGT
                     IVFILGFGISLLAGWHLAPITNGVVQSSGLFGPFESYFGIFLYALICGWIYIRTRNLI
                     ATALVYASLPWFFNFILVSPNKSPTLIGFLFAILVVFLLVLGFVESYRFWAPYITFNI
                     VYEKIETPELENPTTENKEE"
     gene            5675..6058
                     /locus_tag="PK717_00830"
     CDS             5675..6058
                     /locus_tag="PK717_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR46250.1"
                     /translation="MDKKVIKTKNAPEAIGPYSQAIQAGEFIFCSGQIPIDPKTGDHI
                     KSSPSEQARQVLQNITGILEEAGCTLEDVVKITVYLTSMDFFKEVNEIYKEFFKTNPP
                     ARVAVAVSALPLGFNVEMDAIAKTR"
     gene            complement(6061..6990)
                     /locus_tag="PK717_00835"
     CDS             complement(6061..6990)
                     /locus_tag="PK717_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46251.1"
                     /translation="MKRNLAFALLLTIAFASCGTPSYLEGPGETKKEIGAVAPVPNEA
                     KLVEIGEEMQKKIASFNYLTDIEGKINILNKEQREYNVTQASDAGEPEILKNDSEKDG
                     TIEILYSAGSVAILNLAISRITNFQVSELYLPPKATQSDIQSGREMDRMIAERVARAF
                     FTYMGKNLENYKIMSINTGMDGQSKVVFSEILKDEEVPSPNRLVAEIDFWGFVVGFDD
                     GIGPDVTIGTKPATNEEDAIKTAKAYLGMKKDREFINSPKRVVYRKESVENGKLYALD
                     RLVWFFDLIPEKFEGRAALMVDAIAPGKVVGEQ"
     gene            7062..7946
                     /gene="lgt"
                     /locus_tag="PK717_00840"
     CDS             7062..7946
                     /gene="lgt"
                     /locus_tag="PK717_00840"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR46252.1"
                     /translation="MHPIAFFIGNFEIRWYGILITVGIIAGVAYAWTRCKYHGLKKND
                     LYDLAFMGVLVGIIGARLMYIFTNNFSYWLANPLEIFTLQMSGLSFIGIIVFNIPAVA
                     IFTKVRKLSFWRIMDLAAPSIAIGYFFGRLGCFMNGCCYGPDCSWGIVMPGTGKLVPV
                     FPTQLVNAAVAVIMFVVLYWVSTKRKFFQGWLFTLFLYMYTIFHLGTEFLRSDPGHGA
                     LFGTPFNSAQMGNILGLLLAIGLHIYLRRSKIQSTVSDEELVLIEQADKEVAGKKKEE
                     PKEEEPKKPENEEEGKGQ"
     gene            7943..8758
                     /locus_tag="PK717_00845"
     CDS             7943..8758
                     /locus_tag="PK717_00845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR46253.1"
                     /translation="MIPLFDYNLRRANTKRPYVVWSIIIACAIVFIYQITLSPDNNAI
                     FVAKYGMTPGIIAGWELPAGYPYPAIPYLLTLVTSLFLHGDFLHILFNMLYLWVFGDN
                     IENAYGWLGFLVFYITGGVIANLSHMAFSLNPTNAFVPTIGASGAIAAVMGSYLALFP
                     TSKIRTLVIFFFITFVDISAYWWLLIWFGMQFFNLLGGDGGVAWWAHIGGFIYGYIVG
                     FAYLKLFRPYAPQPWGPVITQMKKQSRFFGRRPNQDDNEREDGRYDNQDIWRR"
     gene            8709..9725
                     /locus_tag="PK717_00850"
     CDS             8709..9725
                     /locus_tag="PK717_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46254.1"
                     /translation="MKGKTEDMTTRTFGAVSTLAFFLLVSCGQSPVKPASATVKFVEG
                     ASSGYHSTGKAMLFGDSLIIPVEGSVVSLSLPELEPEWKYDAPTDKGWQCVYVGTASD
                     NIVCVFQSMAGDLTVRLDGRGREILKNQNEKIGSLPKTYSGKIYRVEGEGVFVGEKKF
                     AVPLSLPIIEANGGNIYTVTVEGEVQARNESGDLLWGAKLISRTNNLKAFSWGVVALC
                     ENETIALDQATGSVLWSKPIRATCQPVELGGKLIIAYDKGLMALDRKGNMVYAVDIGC
                     QPIFVSAAPDGIAAICTGKLVTMDLKLTKISEFVVPDGTNQVEFFPGGMVVSGYSQKV
                     FVAE"
     gene            complement(9726..10418)
                     /gene="phoU"
                     /locus_tag="PK717_00855"
     CDS             complement(9726..10418)
                     /gene="phoU"
                     /locus_tag="PK717_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOR46255.1"
                     /translation="MPKNFLLEVETLKKKTLYLSAIVEETFQKTMKAFQERDEATAKA
                     IIEADYEIDTLEVDLEEECLKVLALHQPVASDLRYITSIIKINSELERIGDLACNICK
                     CLLRMAKLPRVDPVPEIELMAERSKIMFRQSLDALMRMDTVLAYKIFSDDEEVDKGKR
                     IVYELVKERIKKNPDDLEAYLNILSVSRFLERIADHATNIAEDVVYMLEGEIVRHKVE
                     GSTASKQHKDNQ"
     gene            complement(10435..11226)
                     /gene="pstB"
                     /locus_tag="PK717_00860"
     CDS             complement(10435..11226)
                     /gene="pstB"
                     /locus_tag="PK717_00860"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOR46256.1"
                     /translation="MIGNQTPPQIDEPEIEIDVQNLDFYYGKHQALHDICISIPKGMV
                     TALIGPSGCGKSTFLRTLNRMNDIIGGTRVEGKIVIQSQDIYAPNIDVVMLRKRVGMV
                     FQKPNPFPKSIFENVAYGPRIHGIKNRAQLQEIVERSLVRAAIWDEVKDRLHISGLEL
                     SGGQQQRLCIARALAVDPDILLMDEPASALDPRSTQRIEDLIAELRGDYTIVIVTHNM
                     QQAARVSDYTAFFYEGHLIEFGKTERIFTKPNNKQTEDYITGRFG"
     gene            complement(11226..>11892)
                     /gene="pstA"
                     /locus_tag="PK717_00865"
     CDS             complement(11226..>11892)
                     /gene="pstA"
                     /locus_tag="PK717_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOR46257.1"
                     /translation="IGTFYLVIGTVAIALPLGIASAIYLNEYSRPSTFTRMIRLGIST
                     LAGVPSIVFGLFGMGLFVLALGFGKSILAGSLTLACMVLPTIIVASEEALKAVPDSMR
                     EGSLALGATKWQTIYRNVLPYALPGMLTGSILGIGRAAGETAPIIFTVAATLLPKLPR
                     SIFDQVMALPYHLYMLATQMPNQTTIKPMQYGTAFVLLVIVLGVDVVAIIFRSRLRRK
                     NKW"
BASE COUNT         2920 a         2696 c         2794 g         3482 t
ORIGIN      
        1 ttttcctgcc atcgtgttga atttatttga ttgccttgtg tcaccagctt cctgcccgcc
       61 tatccttact ttgcatggca gagggcaatt cactgttatt tcgactggtt caccctgttt
      121 gaactccaag accgaggttt ccttgtatga cacgtttatg aattttgtga ccacgtctcc
      181 agttttgctg tatttcacag tgaatttgtg gagcacaggt tcaaggtcga ttggcaggga
      241 aagctcttta tatggtattt ctttggcatc atcgatcgag acaagcgcgt ccggatcgtc
      301 aatcttcagg tccacaaaac caccttttgg accaccgcat gcctggagaa caagcagtga
      361 tgatgcaaaa ataacagaaa gaagcaagca agacaaatgc tttttcatat cagaaacctt
      421 ccttgacgtg tatcacgctg gttttgttgc atgactgata ataatcgcac catggacaga
      481 atttattctt acaccttggg aaaatgccct cttttatctg gccggcaacc gtttcaattg
      541 tattgatggc tgaggcgaga tcatcatcag atggaacaaa tgacatctcg acagacgatt
      601 tcaagtagag atacgttgct ttctcgactt tgttctgata tatactgcca actgccattg
      661 tgtagatggt caactgcagt ttttctgcag gcccaagctc aattggaatg tagttgcctg
      721 ttttatagtc aatgatcctt agtttgtcgt cctctcccct gtcaatgcgg tctattactc
      781 cgtttatggt gatttcatcc atcaccagct tgaatctttt ttctacatca acagcaggca
      841 caatggattg gccatacttt tcaaaatatg aaacaagcag tcctttgcct tcttcttttg
      901 ccttggcttc agtctcgatg tcggcatagc catccgatat ccatgattct tcataccatg
      961 cgacaagttg ggaaagtgaa gatttttcag gaatcagcct tggtttatgg agcttttcaa
     1021 gcgccgagtg gattgaagat ccgaaagaaa agtatggttt tggtaggccg ggtagtttta
     1081 taatgtagct cagataatac ctgtaggggc actccaggta agttgatatc tgggaaaacg
     1141 aaagactcaa agccacttgt tttcttccgc caacttattt atttttttgc agagctcttc
     1201 tttcgtcggt tgggtgtaga cggtccactg cgccagcgcc atgcggtctt tttctgtgag
     1261 ctgtgctgcc attcgtctct gggcgtctct ctcggtcatc cctcttctca tggccctttc
     1321 aagccttaat gattctggcg ctgagatatt gagcacccca tgacatagtt tgtcccattc
     1381 ctgctcaaag aggattgccg ccacgattat tatcaatgac gccctttggg cccttccctg
     1441 cattatctca agtttgattg cgtttgtcag tggttctttg aaaatctcgt tgagcatttt
     1501 tgttctggtt attgttgcaa aagccactct tgccagacgc ttgttgttga tcgagccgtc
     1561 aagattacaa actgattttc caaatttctc aacgatctgg ttgtacacct cacagccagg
     1621 ctgatggagt gggcgcgcca gttcatcgac atcaataacg taggtcctct ctccctgaag
     1681 gcactttgaa gcggttgttt ttccagacga cattccgcct gttattccta ggatgtatgc
     1741 cattattttg cctctagaag gtttttgccc gaatggactt cgcatgtgaa tgggactctt
     1801 gactcacaaa cgttttccat gacctccttg atgagatgga ccatctcatc agtttcgtca
     1861 tcgggacatt caagtacaag ttcatcatgg atttgaagaa ggagtctcga ccttgttttt
     1921 tcaagctttt gttcaagtct aatcatggca agcttgatta tgtcggcggc cgaaccctgg
     1981 attggcgaat tgaccgccgc cctttcacct gctgatctta cctggaaatc ttttgaaaga
     2041 aggtctggca catagcgcct gcggccaagg atggtttccg tgtagccttt tttccttcct
     2101 tgttcgacca cttccttgat gtattttgaa acgccactga agcgctcgaa atattctttt
     2161 ataaaattgt cggcttcctt gaagctgact ccgaattcgt ttgaaagcct gtgggctgac
     2221 atgccataca aaacgccaaa gttgatgatc ttggcttgtc ttctctgggc tggagttaca
     2281 tcactaaccc caaagacaac tcttgcagtg tgggtgtgga tgtcttcgcc tgaattaaac
     2341 gccttcacaa gttcttcatc gccagagagg tgtgcaagca gcctcagctc tatctgtgag
     2401 taatctgccg atacaaaggt tttcccttgc tcggtgacaa aacaacgcct tatctttccg
     2461 ccccagtcag atcttatcgg aatgttttgc aggtttggat tggtcgaaga caatcttccg
     2521 gttgcagttc cagtctggat gtatgttgtg tgaactctgc tctgattgtc acaataggtt
     2581 gggagtgcat caacataggt tgatttgagt tttgagagct ccctgacctc aagtatgagg
     2641 cctatggccg gactggccgg ctttagctgt tcaaggactt cttgtgatgt agaaaagcct
     2701 gtttttgtct ttttgagcgg ctcaaggccc attttctgga aaagcacatc accaagctgt
     2761 tttggtgaga gaatgttgaa tgtggtgttg gctatttcgt aaacttgaga ggatttttct
     2821 gttatccctt tttcaagctg caaggagagt tcgcgaaggg cctctctgtc gatttttatg
     2881 ccccttgttt ccatgtttgc cagcaccctg gacactggct gttcgacatc aatcaggagc
     2941 ccaatttggc ctttctcatc gagatttgtc ctgacccttt cggcaatctt gtgtattgct
     3001 agtccgggtg gtggagaatc atccagatat gcggtgatga gggactctat cgagtagctc
     3061 gatcttccgg gttcgtctat ccatgctgca agctgtacgt caaaaactgg gtcttcaaca
     3121 tcaacggtcc gcaggagttc cttgagatct gtaacaactt tctcgtgttt tgacagaagc
     3181 ggtttctgga gatcttttag tgcctggtca gttttctgga acaggcctga tccgacgtct
     3241 acttcaagga cattattgct gttccatacc tgaagacaac cttcccagca gagcgcaaat
     3301 ggcccgtcaa tgagacttgc agcttcgttg gaagatactt ttcttacggt ggttttttcc
     3361 tgtttcgcaa accttgagac aagcgtcctg aaatcaaaat tgcgcagaaa tgacactgcc
     3421 ttttctggat cgggctgcat tttgaggtcg ttaagtttgt atgacaatgg tacatcatat
     3481 cttatggatg caagcatcag cgagtgtttg aaggcatcga tgttttgggc cacttttgga
     3541 tttttttcga cgtcctcaac agatgatgct tcagaagcta ttttctgggc agttttttcg
     3601 ccaatgccgg gcaccccctg gtagttgtcc gatgggtcac ccttgagagc tttgaagaag
     3661 agaaactggg atggggtcat cccagttttt tcctgcaggg aagcgggcgt gtactcgtca
     3721 atctcggaca tgcctttttt gtttatgtat gcttttatac cgtcatcaag tgtttgaaga
     3781 acatctttgt cgccagtgat gatgatcacc tggttaccat tttctttttc tttttttgca
     3841 acggttgcga tgacgtcatc agcctcaaaa cctgcctgtg aaaggcttgg gatacccata
     3901 tcagtgagga gttttggaaa cataactatc tgggcgatca tgtcttcatc aggtgttggc
     3961 ctgttggctt tatatgaaga atccgcctct tccctgaagg ttttttcctt ccggtcaaga
     4021 caaactgcca cataatccgg atgctctttg tccacgatgt tgtagaacag cctcaagaaa
     4081 ccgtataacg cacctgttgg ggtcccgtcc ttggacgaca tctttggcat tgcatggtat
     4141 gccctgaaaa gcagactcat gccgtcaaga agcaggacct ttgccatcac tcttccttgt
     4201 tttcagtcgt tgggttttct agctctggtg tttcgatttt ctcatatacg atgttgaaag
     4261 taatgtatgg ggcccagaat ctgtaggatt caacaaatcc gaggacgagg aggaatacga
     4321 caagaattgc aaacaggaat ccaatcagag tgggtgattt atttggtgaa accaggataa
     4381 aattgaagaa ccatggcagt gaagcataaa caagtgccgt tgcaatgaga tttcttgtcc
     4441 ttatatatat ccagccgcaa attagggcat acagaaagat gccaaagtaa gattcaaatg
     4501 gcccaaacaa tcctgaagac tggacaacac cattggtgat tggagcaagg tgccagcctg
     4561 cgagcagaga gattccgaaa ccgagaatga agacgattgt tccagggagc cacttcgaaa
     4621 gggaaatacc ccagtcgcca agcggttttt tcctcttctc gatataagcc cacaggaagg
     4681 aaaggttgaa tagtacggca atcagaagcc agaaacagag aagccatatc agattgcctt
     4741 ggctggcccc atattgggtg gcagcttcag tggcgcctga gacctcaata gttgggcttg
     4801 atagcacaag gtctactttg cggtagagtg cgaaaagcag aggcacggaa agtgacgcga
     4861 agaacaacga agaaaagacc ctagaccccg agtggttaga gttatcagtt atatcgtttg
     4921 aggagaacat ggtgaggagc ttgttgaaga agaacccaaa gaccaggaac atgaggattg
     4981 gtccattgag gacccatgtc ctcaagaaat ccagaatgcc cattgtccaa ttgccaggaa
     5041 caacagtctg tgattcaggg ccagcaaatg cccacagtga ttttgtcatg aacatgtctt
     5101 ggagcaacat gccgaagccg gagaaggagt ggaaatcata gtttgatgtg ccgggtggcg
     5161 aaggaatacc aagatagaac gtatcaccgc cggtgaataa agagccgatt ggcacgaata
     5221 tgaaatagat aattgcccac atcaccgcaa aaaagagtac tccagggaga atcctgttcc
     5281 agtggagtcc ccacaagttg aggtttggtc tttctttgag gatgaggtat atgaggaaga
     5341 aggccaggaa gatgaggaaa ataacgttga acatccatct tccataaaaa tcggccatgc
     5401 cccttgcata atgcgtatac tcaaggcttt gtccataaag gttgttcaag ttgtacatct
     5461 tggttacaac agaaaccact acgggctgga aaatccatct tacaagtgcc ggtattccca
     5521 ggaaaacaag gatgtattga agccatgtgg catcagcaaa ccacttccag gcggtcttaa
     5581 agccgtcttt tacactcata ctacctccat tgtggaaaaa acaaaactaa cgtattatcc
     5641 tacacacata cacacacaaa ggcaaggagg ttgcatggac aagaaggtta tcaagacaaa
     5701 aaacgcgcca gaggcgattg gcccatattc acaggcaata caggcagggg aatttatttt
     5761 ctgttctggc cagattccga ttgacccaaa aaccggtgat cacatcaaaa gttctccatc
     5821 agagcaagcc agacaggtgt tgcagaacat aaccggcatt cttgaagaag caggatgtac
     5881 tctggaggat gttgtgaaaa ttaccgttta tcttacctca atggacttct tcaaggaagt
     5941 gaatgagatc tacaaggaat ttttcaaaac caatccgcca gcaagagttg ctgtggcagt
     6001 ttcagcattg ccgcttggct tcaacgtgga gatggatgca atagcaaaga ccaggtaatt
     6061 ctattgctcg ccgaccacct tccctggtgc tatggcgtca accattaaag cggctctgcc
     6121 ctcaaacttc tccggaatca agtcgaaaaa ccataccagt ctgtcgaggg catacagttt
     6181 tccgttctcg acagactcct ttcgatagac cactctcttc ggtgagttga tgaattccct
     6241 gtcttttttc atgccaaggt atgcttttgc agtctttatg gcgtcctctt cgttggttgc
     6301 tggtttggtt ccgatggtca catccggccc aatcccgtca tcaaagccaa ccacaaaacc
     6361 ccagaaatct atttcagcaa ccaatcggtt tggtgatgga acttcttcat ctttgagaat
     6421 ttcggagaaa acaacctttg actggccatc cattccagta ttgatcgaca ttattttgta
     6481 attttcaagg tttttgccca tatatgtgaa gaaagctctt gccacacgct cggctatcat
     6541 cctgtccatt tcccttccag actggatgtc gctttgtgtt gcttttggtg gcaaataaag
     6601 ctcggatacc tggaaatttg taattctgct gatggcaagg ttcagtattg caacactccc
     6661 agcgctgtaa agaatttcga tggttccgtc tttttctgaa tcatttttca gtatctctgg
     6721 ctctcctgcg tcgcttgcct gggtcacgtt atattctctt tgctctttgt taaggatgtt
     6781 tattttacct tcaatatcag tgaggtagtt gaacgaagcg atcttctttt gcatctcttc
     6841 gcctatctcc acgagctttg cctcatttgg aactggggcc acagcaccta tttctttttt
     6901 tgtttcacct gggccttcaa ggtaagatgg tgtgccgcat gaagcaaagg ctattgtgag
     6961 tagcaacgca aaagctaggt ttcttttcat ggggcaaata ataaatacac aatcgatttt
     7021 tttcaataat atgttataaa agttgcgtac aaggaggaca gatgcatcca attgcatttt
     7081 ttattggcaa cttcgagatt cgctggtacg gcatcctgat tacagttggg attattgcag
     7141 gtgttgccta cgcttggacc aggtgcaagt atcatggcct gaagaaaaat gatctttacg
     7201 atctggcgtt catgggtgtt ctggtaggca taattggtgc aaggctgatg tacatattca
     7261 caaacaattt ctcgtactgg cttgccaatc ctcttgaaat attcacactg cagatgtcag
     7321 gcttatcatt tatcggcatt attgtcttta acatacctgc cgtggccata tttaccaaag
     7381 tgcgaaaact gtctttctgg agaataatgg atcttgcggc accaagcatt gcaataggct
     7441 atttttttgg caggcttggc tgtttcatga atggttgttg ctatggccct gattgctctt
     7501 ggggtatcgt gatgcctggg actggaaaac ttgtgccggt ttttccaaca cagcttgtga
     7561 atgccgccgt tgctgtcatc atgtttgttg tgctctactg ggtatctaca aaacgcaaat
     7621 tcttccaggg ctggcttttt acacttttcc tctacatgta cactatcttc catcttggaa
     7681 cagagtttct aagatcagac cctgggcatg gggccctttt tgggacgcca ttcaattctg
     7741 cccagatggg gaacatattg gggcttctct tggcaatagg cctacacatc tacctgagaa
     7801 ggagcaaaat ccaatccaca gtttccgatg aagagttggt gctcattgaa caagcagaca
     7861 aggaagttgc tggcaaaaag aaagaggagc caaaagaaga agaaccaaaa aaaccagaga
     7921 atgaagaaga gggcaagggg caatgattcc attatttgat tacaacctca ggagagccaa
     7981 taccaaaagg ccttatgtcg tttggtcgat cattattgcc tgcgcaatag tttttatcta
     8041 tcaaataaca ctttctccag acaataatgc catctttgtt gcaaaatatg gtatgactcc
     8101 tggcataata gcaggatggg aattgcctgc cgggtatccc tatcctgcaa tcccatacct
     8161 gcttaccctt gtcacatcac tgtttcttca tggtgacttc ctccatatcc tcttcaacat
     8221 gctttatcta tgggtttttg gcgacaacat agaaaatgcc tatggctggc tgggttttct
     8281 ggttttctac atcactggtg gtgtgatagc taatctctcg cacatggcct tttccctcaa
     8341 cccaacgaat gcttttgtcc cgactatcgg tgcttctggg gccattgcgg ccgtcatggg
     8401 ctcgtatctt gccctctttc caacatcaaa aataagaact cttgtcattt tcttctttat
     8461 aacgtttgtt gacatctcag cctactggtg gctcctaatc tggtttggaa tgcaattctt
     8521 caacctcctt ggtggagatg gaggcgtggc ctggtgggca cacattggtg gcttcatcta
     8581 tggatacatc gttggttttg cctatcttaa gcttttcagg ccatacgcgc cacaaccgtg
     8641 ggggccagtg attacccaaa tgaagaagca atcaaggttt tttggcagga gaccaaacca
     8701 ggacgataat gaaagggaag acggaagata tgacaaccag gacatttggc gccgttagca
     8761 cgttagcctt ttttttgttg gtctcatgcg gccaatcgcc agtaaaacct gcatcagcaa
     8821 ccgtcaaatt tgtggaaggc gcgtcatctg gttatcattc aaccggaaag gctatgctgt
     8881 ttggtgatag cctgataatc cctgttgaag gttctgtagt ctcgctttcc ttgccagaac
     8941 ttgagccaga gtggaagtat gatgcgccaa cagacaaagg ctggcagtgt gtttatgttg
     9001 gaacagccag tgataatatt gtttgcgtct tccagagcat ggccggagac ctgactgtcc
     9061 gtcttgatgg cagaggcaga gaaatcttga agaaccagaa tgagaaaatt ggttccctgc
     9121 caaaaacgta ctctgggaag atttacaggg tcgaaggcga gggtgttttt gttggagaaa
     9181 aaaagtttgc tgtgccgctc tcgctcccca ttatcgaagc caatggcggt aatatctata
     9241 cggtcacagt tgagggtgaa gtccaggcaa ggaatgagtc cggtgacctc ctctggggag
     9301 caaagctcat ttccagaaca aacaatctga aggcattttc gtggggcgtg gttgcgctct
     9361 gcgaaaacga aacgattgca cttgatcagg ccacgggcag tgtcctctgg tcaaaaccca
     9421 taagggccac atgccagcca gtggagctgg gtggcaagct gataatcgcc tatgacaagg
     9481 ggctgatggc cctggacaga aaaggtaata tggtttatgc agtcgatatt ggatgccagc
     9541 ccatatttgt ctctgccgca cctgatggta ttgcggccat ctgcactggc aaattagtta
     9601 caatggacct taaactcaca aaaatatcag aatttgtcgt cccggatggg acaaaccagg
     9661 ttgagttttt tcctggcggg atggtcgtat ctggttatag ccagaaggtt tttgtggcag
     9721 aataattatt gattgtcttt gtgttgcttg ctggcagtgg agccttccac cttgtgtctt
     9781 actatctcgc cctcaagcat ataaacaacg tcttcggcga tatttgttgc gtggtctgcg
     9841 atgcgctcaa gaaacctcga tacagagagg atgtttagat aggcctcaag atcatcaggg
     9901 ttctttttga tcctctcttt tactagttcg tagacgattc tcttgccctt gtccacttcc
     9961 tcgtcatcgg agaatatctt gtaggcaaga accgtgtcca ttctcatcag ggcatcgagg
    10021 ctctgcctga acattatttt tgagcgttcc gccatcaatt cgatttcagg gactggatcg
    10081 acccttggca atttggccat tctgaggagg cacttgcaga tattgcaggc aaggtcgcca
    10141 atcctttcaa gctcgctatt gattttgatt atagaggtga tgtacctgag atctgaagca
    10201 acgggttggt ggagtgccaa caccttgagg cattcctctt ccaggtccac ttcaagggtg
    10261 tctatttcgt agtcggcctc gatgattgct tttgctgtgg cttcgtccct ttcctgaaag
    10321 gccttcatgg tcttctgaaa agtctcttcg actatcgctg agaggtaaag tgtctttttt
    10381 ttcagggtct ctacttcaag cagaaaattt tttggcattt tctaaccctc caaatcatcc
    10441 gaagcgaccg gttatgtagt cctcggtttg cttgttattt ggttttgtga atatacgttc
    10501 tgtctttcca aactcaatta aatgtccttc ataaaagaaa gctgtgtagt ccgaaactct
    10561 tgccgcttgt tgcatattgt gcgtaacgat gacaattgtg taatcacccc gcaactcggc
    10621 aataagatct tctattcttt gggtagacct tggatcaagg gccgaggcag gttcgtccat
    10681 taacagtatg tcggggtcaa ccgcgagtgc cctggcgatg caaagcctct gttgctgtcc
    10741 gccggagagc tcaagcccgc ttatgtggag tctgtccttg acctcgtccc atattgcggc
    10801 cctcacgagg cttctttcca cgatctcctg gagttgtgcc ctgttcttta tgccgtgtat
    10861 ccttggtcca tatgcaacat tctcgaaaat cgattttggg aaagggtttg gtttctgaaa
    10921 gaccattcca acccttttcc tgagcatgac cacatcgatg tttggtgcgt agatgtcctg
    10981 tgactggatg acgatttttc cctctactct tgttcctcca atgatgtcat tcattctgtt
    11041 cagggtgcgc agaaaagtcg attttccgca ccctgaaggg ccgataagcg ctgtaaccat
    11101 gccctttggt attgatatac aaatatcatg gagggcctgg tgtttcccgt aataaaaatc
    11161 caggttctgg acatctattt caatctctgg ttcatcaatc tgcggtggtg tctggtttcc
    11221 tatcatcacc atttgttttt cctcctgagc ctgctgcgga aaatgattgc cacaacatct
    11281 acgccaagga ctatgacgag caggacaaag gctgtcccgt attgcattgg ttttattgtg
    11341 gtctggttgg gcatctgggt tgcaagcatg tataggtggt atggcagggc cattacctga
    11401 tcaaatattg accttgggag ctttggaagg agtgttgctg caactgtaaa aatgatcggg
    11461 gcggtttcac ccgcagccct tccaatgcca agtatcgagc cggtgagcat tccagggagt
    11521 gcgtagggaa ggacgtttct gtagatcgtc tgccattttg ttgcgccgag agcaaggctg
    11581 ccctccctca tcgagtctgg gacagccttc aatgcctctt cggaggccac tatgatggtt
    11641 ggaagaacca tgcaggcaag cgtaaggctg cctgcaagta tcgatttgcc aaagcccagc
    11701 gcaagcacaa aaagccccat tccaaaaagc ccaaaaacaa ttgaaggaac gccggccagt
    11761 gtacttattc caagccttat catccttgtg aacgtggacg gccttgaata ttcattgagg
    11821 tatatggccg aggcaatgcc aagcgggagt gcaattgcca cggtcccgat caccagatag
    11881 aaagtaccta tg
//
LOCUS       DAOSRI010000015        11799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5410_length_11799_cov_4.083447, whole genome shotgun sequence.
ACCESSION   DAOSRI010000015 DAOSRI010000000
VERSION     DAOSRI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_11799_cov_4.083447"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK717_00870"
     CDS             <1..507
                     /locus_tag="PK717_00870"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOR46258.1"
                     /translation="TAGRGRQGKTWASSIGGLWTTIILRREVPLDLVPYIALASGLVI
                     KKALLERVGINATIKYPNDLLFDCNGEWKKLCGILCQSSIVSGQLRYVLVGVGLNVNN
                     KTPSYATSLKQITGKEHDLDLMAKIVSEAVSFGTLKLVEHGKMKVIKDLFNDGAIEMP
                     LDNIFENE"
     gene            520..738
                     /locus_tag="PK717_00875"
     CDS             520..738
                     /locus_tag="PK717_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR46259.1"
                     /translation="MPIYEYTCPKCGHDFEVFTVKIEEGTPSCPKCGYEKTKKRISML
                     GYLKHPDHGGQGKLTSGSTGCSDGSCSK"
     gene            719..1978
                     /locus_tag="PK717_00880"
     CDS             719..1978
                     /locus_tag="PK717_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOR46260.1"
                     /translation="MAPVQNKTKAQKKENPYLKAFLSWFGKELGKSPNSVKAYHWELS
                     RFFNYLGQNDITIPECKRLHIRDFLTELNQDNSSHTRARTLFCIKTFFRFLYREGYIP
                     SNPAETIDPPKQTQKVPKFLLANQFRKLIKELCLEAEKENNTQKKVLKVAYESEPGPD
                     SFFILLRMLGLNMEEISSLSVSKINIRSKTISLNENKKILLDQKSSACVAQFINNQEL
                     QKSFFEGNSRKKAFKNHISWLESNGATQEEATHGLESFRVNPAKRDLAIISLLLGTGI
                     RRSELVGLNRQNYNEESKTIMVTRKGGDQQIVEVGDEVCVALSRYLGTRRDNLDPMFL
                     SAKGKRLSNEGLWLITKKILGKFGLEGSTHTLRHTFVTELVRQGVPVAIVQSVVGHKS
                     AQTTMRYTHIISSDRRNAISKIKLGIK"
     gene            2034..2549
                     /locus_tag="PK717_00885"
     CDS             2034..2549
                     /locus_tag="PK717_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOR46261.1"
                     /translation="MIECPVCGRANLGGVLCCESCGAKLEQKDEHFENVLQNPNLIRF
                     PHPPSTVTSSHPAIKTQVEMVGRLALNDEHQFILDGKNEYLIGRADKLAGIVPEIDLT
                     NFDKEMVTSRRHACVTKKGDSYIIEDLGSTNFTYLNGRMLSPKTPTKLDDGDVIRIGK
                     VYLVFSRIKKN"
     gene            2571..4976
                     /locus_tag="PK717_00890"
     CDS             2571..4976
                     /locus_tag="PK717_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR46262.1"
                     /translation="MENRVIVKKYKLLEPLASGGMATVHLAKNLATDEIVVAKIPNFS
                     GLPNKEKFERRFLREAQILSKINSRYVVKIHDFGKEESTGEYFLILEYLHGKTLEEIA
                     QGKERIPVPTVVDLTMQIAEVLGDLHDQGVVHRDIKSSNIKISSEGNIKLFDFGISKG
                     EDLPSMTRSSDFLGTLQYMSPEQTDGREVDIRSDIYSTGIVIYEMLFGKLPFDAPSPV
                     EVIEMQRHKQPKIPPEAKERGIPASLLSLMFRCLEKNPADRFQTPQELAAALRAVSED
                     LGMTQSERDAYRKTKLTQIALSSPQSTFRTRARRKQTMTIALTVAGIIAISSGIWLSR
                     GCTKPLEPDFLVAQGELVQYQIPLATPPGTKDIEATISKSPDGLSVKLEKQMENEGEN
                     WVLSIYSNLTMPIGVYEIKLGVQYTRDNDLKENEETSFNVGVIKGNLGNKAIRLISSD
                     EIPNDPNSKVENAVKINGKIFVPVETIASGTKAQTEWDRESGKLTYWTPDKTIELKKG
                     EKTLKENGSEKPLNEAPVVLNDSMMVSGNVAETYMDTAVTSKNDSEIELTFKQSTPGS
                     KISISTKDEKGNDIKGAIVLINQNYKGKTPIKIELPNGVYQIKIQMGGFNDINDEIKL
                     VTTKTLDLSYPLVAIQQKPPEQEKKPPVETKPKQNGFLDLSVNVPWGSFIVDGKTQQN
                     ITSLSLELAPGKHKVVYRLKDFPDQTKDVQIEPGRRNSVKFQISSGILKVTCNVVGIV
                     YIDNIEFSDRIFTSPKEPYIREVAAKTYIIRVDKSGYKMQTKSVKVEPGKTTTVEFNL
                     EPK"
     gene            4973..7636
                     /locus_tag="PK717_00895"
     CDS             4973..7636
                     /locus_tag="PK717_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46263.1"
                     /translation="MRRNFLLLAVISCLFATGISLAKPSFDWWPMIGHDNLKSGISKD
                     NYLPFTLPFSESKKGSWDVSLSNLGGLGTPVVKDGVLYIVQQGFYDKRSGNRQEGTLR
                     QYDIFTGNLLLEQTFNAVDVPEPGVVGSPAIWGGNEEKTVVVGDYLGNIVYFRGIFPK
                     KLVDAGGERTPITSSPTIYDNTCYIGSDSGNVYAFDLAELRITDSFKAKGKITTSPSA
                     LKDLIVFGDDSGSFFAYNRKTGKQIINAQPSTRDDISITSSSCITESQGKTYAIFGSS
                     KGFVHRITIGGSNIGQIVSFRTSPDRRTQRPTFWATPTVVDDQIYAGADDGNLYRIDL
                     GTMKSAGMIDLKNPIFAQATSAGKYLYVCTASLDSEENPAREGSLYTIDLDTFTINQT
                     NDSGIEIKGGSLTQPVIAGGYMFVASRSGKIYRFEGARPQLMVSPTQINFGTIKFGTA
                     GQKDIVISNNGPGDPPLVGEILVDEQSKLWLSVDKSEFKTSGETKLTVKASSSNIKDI
                     RETEYSGTISIETNAGIQTIEVKATFAPDLPRIQVSSSQIDFMDTYRGRKNLREFTVS
                     LAKGSMATLDFKPSENWLECSPQSATLSEGKKSIKISVTASTENLPIGKYAATIQIGD
                     QKTIANPVTLPVTINVVPQPARPSIVNSSVEVVFQDCFVDKPKEAVFQILNSGDNDLK
                     EGIKPILSTSWLSNPRFVKNKGNSWLYICTISPSTFWPNQQYTASINTMISSTPITLK
                     ITVKTMHIPECKIEFSIGSKQYIVNGRSLPMDAPPYVSKNGSTMVPIRYIGDPLGYFY
                     KAQTEWVKELKTVLFTLAGTTLRLIVDYQNAIVERADGSIDTVKLASPPEIKNGRTFI
                     PPRIIGETFGAKISWDDRTRKATLVFNQPPP"
     gene            7726..8067
                     /locus_tag="PK717_00900"
     CDS             7726..8067
                     /locus_tag="PK717_00900"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOR46264.1"
                     /translation="MKYLDEENERELQEISDIFSALSSPKRLKIISLIEDGPLSVTEI
                     AQKMKCRPPCVSQHLSVLKSCKIVDCERTGHEMVYKLALSCVKSFISCLLGKCDSNDS
                     GCSIVKSVHAK"
     gene            8085..8324
                     /locus_tag="PK717_00905"
     CDS             8085..8324
                     /locus_tag="PK717_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR46265.1"
                     /translation="MKIQVLGMGCAKCHSLEANVREAVAKMGIDAEIEPIKDIQKIVE
                     MGVMATPAIAIDGKVVSSGRMLSPSQITELIKQNQ"
     gene            8324..9808
                     /locus_tag="PK717_00910"
     CDS             8324..9808
                     /locus_tag="PK717_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46266.1"
                     /translation="MKNTTKILLSILVLVIAIVNTNAMFQPGIPDNGLVANDDWASSP
                     QSQVWEKQLQTDIVSGPVFIGSRVAVALGNSQVNAFSIYSGKLAWSYKLDQKPNQIAS
                     GSDSVFVKTARQVTSIGDGGQKNWIFQTEANITTDLAILGNMLFFGCADGNFYCVNTK
                     NGKKIWLSAFKSIPNIGPKMLGEDYLFFASTDKKLYCLNTSSGKQKWELALDDSPEWI
                     FADQNRVLAQEGKYLFAIEAQSGNPRWKYETGTGSLQAVSSKGKFFVTTPALAIDCFD
                     ANFFGKACCISTGKRLWLSQLDSFPTTRLFCSEETIFVPKGNLIEKISVADGSKQGNI
                     TLDKKIESIDLQGDNIAIISGNTISLFNKVPEKITFVVGQKELGRDSFAVEMDVAPVI
                     IDGKSFLPARYVCEPFGGSVSWDQGSKKTTCQLNGKTLSLWVGKNTARTSGGDKQIDS
                     NPKIIPFIQNGRVMLPMRFLAESLGLKVGYENKKISIENERGQK"
     gene            9805..10227
                     /locus_tag="PK717_00915"
     CDS             9805..10227
                     /locus_tag="PK717_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR46267.1"
                     /translation="MRRNILSSFVLLAVFAGSIFLASCGSNGNLPCVSEGETGNGTIS
                     IKNDSGMPMVVKVGRATCKPCIVMTDTVNEIGKELEGKIQIAIVDIDEDPEAVEKFQI
                     QGIPTTFVFDASGNQTFRKTGIATKDELVSELKKAGMQ"
     gene            10224..10628
                     /locus_tag="PK717_00920"
     CDS             10224..10628
                     /locus_tag="PK717_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOR46268.1"
                     /translation="MKNNITNTIILVIVALAALGLLSYKLFFEKGSDFPKPQKGEVTI
                     VELGADLCEPCTKLRPIMEDLKKELEGKAVLNSLLIDNSMKSFYNVKVIPVIIIFDKE
                     ANEVARRIFSLEEVDDARNWIKEKTEPLGVQW"
     gene            10625..11308
                     /locus_tag="PK717_00925"
     CDS             10625..11308
                     /locus_tag="PK717_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOR46269.1"
                     /translation="MIESLLQSIQSNPWIAPFTVFFTGFLTASNPCVLGMIPLMIGAS
                     GAYQGEDKSYWKAAKFSLLFVLGQSISFMVLGIIAALIGQQATLTGPWWTYFMAFLCI
                     LMGLMFADIVKFNIPVPKILTKPRTGLFGAFILGVLFGFVSTPCAVPILSVLLALIAT
                     GGNTYFGGLLLLCYALGHSALLLVAGISIGAAQAFINNRNLRTTSLVLRKVFGLLIIG
                     FGFYLLYIA"
     gene            11324..>11799
                     /locus_tag="PK717_00930"
     CDS             11324..>11799
                     /locus_tag="PK717_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR46270.1"
                     /translation="MDIFAPVNWFAKWLTYDIFRIQHESLLGEAVSFFVFDTIKVFML
                     LIAIIFVVSYLRTYLPPEKVRFFISKRNKITGHIMAAAFGIITPFCTCSAIPLFLGFL
                     EAGVPLGVTFSFLIASPMINEVALVMLLGMFGWKVALIYIASGMIIAILSGLVIGQL"
BASE COUNT         3830 a         2577 c         2584 g         2808 t
ORIGIN      
        1 actgctggca gggggagaca gggaaaaaca tgggcttcgt ccatcggcgg gctctggaca
       61 acaataattc tgcgccgcga ggttcctctg gaccttgttc cttatatcgc actggcatca
      121 ggattggtaa taaagaaagc tcttctggag agagtgggca taaacgcgac aatcaaatat
      181 ccaaacgacc tgctttttga ctgtaatggc gagtggaaaa agctctgtgg aatactttgc
      241 cagtcatcaa ttgtttccgg gcagctccga tatgttctgg ttggcgtggg attgaatgta
      301 aacaacaaaa ccccttctta tgcaacctcc ctgaaacaga taactggaaa agagcatgat
      361 cttgatttga tggcaaaaat cgtctccgag gccgtgtctt tcggaacatt aaaattggtt
      421 gaacacggaa aaatgaaggt cattaaagac ctttttaacg atggagcaat cgaaatgcca
      481 cttgacaata tttttgaaaa tgaatagtaa attatcacca tgcctatata tgaatacact
      541 tgcccaaaat gcggacacga cttcgaagtg ttcacagtaa agatagaaga aggaacacca
      601 tcctgcccaa aatgtggcta cgaaaaaaca aaaaagagaa tatcaatgct tggctacctg
      661 aagcatccgg atcacggtgg acaagggaag ctcacatccg ggagcaccgg ctgtagcgat
      721 ggctcctgtt caaaataaaa caaaagcgca aaaaaaagaa aacccttatc ttaaggcatt
      781 tctttcatgg ttcggaaaag agctgggcaa aagcccgaat tcagtaaaag cttaccactg
      841 ggaactttcc cgctttttca attaccttgg ccaaaacgac atcaccattc cagagtgcaa
      901 aagattgcac ataagagatt ttctcacaga actcaaccag gacaattcat cacacacaag
      961 ggccagaacg cttttttgca tcaaaacgtt cttcaggttc ctctacaggg aaggatacat
     1021 accgtcaaac cctgccgaaa caattgatcc acccaagcag acccagaaag tgccaaaatt
     1081 tttgcttgca aaccagtttc gaaagctgat aaaagagctc tgccttgagg ctgagaaaga
     1141 aaacaacaca caaaagaagg tcctgaaggt tgcctatgaa tcggagcctg gcccagattc
     1201 attttttatt cttctgagaa tgcttggatt gaacatggaa gaaatatcct cactctctgt
     1261 ttcaaaaata aacatcagga gcaaaaccat ttctcttaat gaaaacaaaa aaatcctgct
     1321 ggaccaaaaa tcatctgctt gtgtggccca attcataaac aatcaggaac tgcaaaaatc
     1381 tttttttgaa ggtaacagca gaaaaaaagc tttcaaaaac catatttcat ggcttgaatc
     1441 aaatggcgcc actcaagaag aagcaaccca tggactggaa agttttcggg taaacccggc
     1501 caaaagggac ctggcgatca taagcctttt gctgggaact ggaatcaggc gaagcgagct
     1561 ggttggcctg aacaggcaaa actacaatga agaatcgaaa accatcatgg tcacaagaaa
     1621 aggtggcgac cagcagattg tcgaggtcgg cgatgaagtc tgtgttgcgc tttctcgcta
     1681 ccttggtaca cgcagggaca accttgaccc aatgtttctt tctgcaaaag gaaaaaggct
     1741 cagcaatgaa ggtctctggc tgattaccaa gaaaatcctt ggaaaatttg ggcttgaagg
     1801 ttccacacat acactcaggc acacgtttgt aaccgaactc gtcaggcagg gagttcctgt
     1861 ggcaatcgtt caatctgtgg tcggacacaa aagcgcacaa accacgatga gatacactca
     1921 tatcatatct tcagacagaa gaaatgccat ttccaagatc aagctcggca taaaataggg
     1981 ccactccgtt tacaacagct ctttatgatg tataattcga ggatggaggt aaaatgatcg
     2041 aatgtcccgt ttgcggcaga gcaaaccttg gtggtgtttt gtgttgcgaa tcgtgcggag
     2101 caaagctcga gcagaaagat gaacattttg aaaatgtcct ccagaacccg aacctgataa
     2161 ggttcccgca ccctccaagc actgtaactt catcgcatcc tgccatcaaa acacaggtgg
     2221 agatggttgg aaggttagca cttaatgacg agcaccagtt catccttgat ggcaaaaacg
     2281 aatatctgat aggcagagct gacaaattgg cagggatagt tcctgagatc gaccttacta
     2341 atttcgacaa ggagatggta acatcaagaa ggcacgcctg tgtgacaaaa aaaggcgact
     2401 catacataat tgaagatctt ggctcaacaa attttaccta tctgaatgga agaatgctct
     2461 ccccaaaaac ccccacaaag cttgatgacg gggacgttat caggatagga aaagtttatc
     2521 tggtgttttc aagaataaag aaaaactagg atttttattg gaggaaacct ttggaaaaca
     2581 gggttatagt caagaaatac aaattgcttg aacctctggc atctggtgga atggcaactg
     2641 ttcacttggc aaaaaatctt gccacagacg agattgtcgt ggcaaaaata cccaatttct
     2701 cggggcttcc aaataaggaa aagttcgaaa gaaggtttct gagagaagcc cagatactct
     2761 caaaaataaa cagcaggtat gttgtcaaaa tacatgattt tggcaaagag gagtcaactg
     2821 gagagtactt ccttattcta gaatacctcc atggcaaaac actcgaggag atagctcaag
     2881 ggaaagaaag gattccagtt ccaacagttg ttgacctcac aatgcaaatt gcagaggtac
     2941 tgggtgacct gcacgatcaa ggagttgtcc acagggacat aaaaagctcc aacatcaaaa
     3001 tatcttccga aggcaacatc aagctcttcg attttggaat aagcaagggc gaggacctcc
     3061 caagcatgac caggtcttct gacttcctgg gaacactcca gtacatgtcg ccagagcaga
     3121 ctgatggaag ggaagtcgac ataaggtctg acatatactc gaccggcatc gtcatttatg
     3181 aaatgctgtt tggcaagttg ccttttgatg cgccatcgcc tgttgaagta atcgaaatgc
     3241 agagacacaa acaaccaaag ataccccccg aagccaaaga aaggggcatt ccagcttctc
     3301 tgctgtcctt gatgttcaga tgtcttgaaa aaaatcctgc cgacaggttt caaacccccc
     3361 aagagctggc cgcagcattg agggcagttt ccgaagattt ggggatgacc caatctgaaa
     3421 gggacgccta tagaaaaacc aaactcactc agatagccct ttcttctccg caatcaactt
     3481 ttagaacacg ggcaagaaga aaacaaacca tgacaatagc cctcacagtc gcagggatta
     3541 ttgccatatc ttcaggtatc tggctatcaa ggggttgcac aaaaccactg gaaccagact
     3601 ttctggttgc ccagggcgaa cttgtgcaat accagattcc gcttgcaaca ccacctggaa
     3661 caaaagatat agaagccaca ataagtaagt ctccggatgg attgtcagta aagcttgaaa
     3721 agcaaatgga aaacgagggt gaaaattggg tcctctcaat atattccaac ctcacaatgc
     3781 ccataggtgt ttatgaaata aagcttggcg tacaatacac aagagataac gatctcaaag
     3841 aaaatgagga gacaagcttc aatgttgggg taataaaagg caatcttggc aacaaagcaa
     3901 tcaggttgat cagttcggat gaaatcccca acgatccgaa ctcaaaggtc gagaatgccg
     3961 tcaaaatcaa tggaaaaatc tttgtgcctg ttgaaacaat tgcatcaggg acaaaagctc
     4021 agaccgaatg ggacagggaa tccggaaagc tgacatactg gaccccagac aagacaatcg
     4081 agctcaaaaa aggtgaaaaa actctgaaag aaaatggctc ggagaagcca ttgaatgaag
     4141 cgcctgttgt gctcaatgac tcaatgatgg tctccggtaa tgttgccgaa acgtacatgg
     4201 acacagctgt gacatcaaaa aatgattctg aaattgaatt gacattcaaa cagtccacgc
     4261 ctggctcaaa aatttcaata agcaccaaag acgaaaaggg caatgacatc aaaggcgcaa
     4321 ttgtattgat aaaccagaac tacaagggca aaacaccaat aaaaatagaa cttccgaatg
     4381 gcgtctacca gataaagata caaatgggcg gttttaacga tataaacgac gagataaaac
     4441 ttgtgacaac caaaaccctt gatttgagct atccactggt cgcaatacag caaaaaccgc
     4501 cagagcagga aaagaaaccg ccagtagaaa cgaaacccaa gcaaaacggg tttcttgatc
     4561 ttagcgtcaa tgtcccatgg ggctctttca ttgttgacgg aaaaacccag caaaatatta
     4621 caagcctttc actggagctt gcaccgggca aacacaaggt tgtatacagg ctaaaagatt
     4681 tccccgacca gacaaaagat gtccagatcg aaccagggcg aagaaactca gtcaaattcc
     4741 agatttcctc aggcatcctc aaggtgacat gcaatgtggt aggcattgta tacatagaca
     4801 acattgagtt ctcggacagg attttcacaa gtcccaagga accatacata agggaagtgg
     4861 ccgcaaaaac gtatataatc cgcgtcgaca aaagtggata caagatgcag acgaaatctg
     4921 taaaagttga gcctggcaaa accacaacag tggagtttaa cctggaacca aaatgagaag
     4981 aaactttctt ctattagcag ttatttcatg tctctttgca acgggtatat cacttgccaa
     5041 accatctttc gactggtggc caatgatagg gcatgacaat cttaaaagtg gtataagcaa
     5101 ggataattat ctccctttca cgctaccttt tagtgaaagc aaaaaggggt cctgggacgt
     5161 atcactttca aatctcggtg ggctgggaac accggtcgta aaagatggag ttttatatat
     5221 tgtccaacag ggtttttacg acaaaaggag tggcaatagg caagagggaa ctctcagaca
     5281 gtatgacata tttaccggga accttctcct agaacaaact ttcaatgccg tggatgtacc
     5341 agagccagga gtggttggga gtcctgccat ctggggagga aatgaagaaa aaacagtcgt
     5401 ggttggagat tatcttggaa acatagtcta tttcaggggt attttcccaa aaaaacttgt
     5461 tgatgcaggc ggggagagaa caccaatcac ttcgtcgcca accatatacg acaatacctg
     5521 ttacattggt tccgattcag gaaatgtcta tgccttcgat cttgccgaat taagaataac
     5581 agattcattc aaggcaaaag gaaagataac aacttcgcca tctgcgttga aagatctcat
     5641 tgtttttggc gatgattcgg ggagtttttt tgcatacaac agaaaaaccg gcaagcagat
     5701 cataaatgcc cagccatcaa caagagatga catatcaata acttcatcat catgtattac
     5761 tgaatcacaa ggaaaaacat atgcgatttt tggttcctca aaaggttttg tgcaccgaat
     5821 aacaataggc ggaagtaata tcgggcaaat tgtaagtttc aggacaagcc ccgacaggag
     5881 aacacagagg ccaactttct gggcaacgcc aaccgttgtg gatgaccaga tttatgcagg
     5941 cgcagacgat ggcaacttgt acagaataga cctgggaaca atgaaatcgg ccggaatgat
     6001 agatttgaaa aatccaattt ttgcgcaagc aacctcggca ggcaaatacc tctatgtttg
     6061 cactgcatcg cttgacagcg aagaaaatcc tgccagagag ggaagcctgt atacaattga
     6121 tcttgataca ttcacaatca atcaaaccaa tgacagtggt attgagatca agggtggctc
     6181 cctcacacaa ccggtgatag ctggcggata tatgtttgtg gcctccaggt cgggcaaaat
     6241 ttaccgtttt gagggcgcca ggccacagct aatggtttca ccaacccaaa tcaattttgg
     6301 taccataaaa ttcggcacag caggacaaaa ggatattgtc attagtaata atggtcctgg
     6361 cgacccgcca cttgtcggtg aaatcctggt ggatgaacag tcgaaactat ggctttcagt
     6421 cgacaagtcg gagttcaaaa cttctggaga aacaaaatta acagtaaaag caagttcttc
     6481 taatatcaaa gacatcaggg agacagagta ttcaggcact ataagtattg aaaccaacgc
     6541 cggaatacaa accattgaag taaaagcaac ttttgcgcct gatttgccaa gaatacaagt
     6601 ctcaagctcc caaattgatt tcatggacac atacagagga cgaaaaaatt tgagagagtt
     6661 tactgtaagc ctggcaaaag gatcaatggc aactcttgat ttcaaaccct ccgaaaactg
     6721 gctcgaatgc tctccacaat cagcaaccct ctctgaaggg aaaaaaagca taaaaatatc
     6781 agtgactgct tcaactgaga accttcccat agggaaatat gcggcaacaa tccagatcgg
     6841 ggaccagaaa acaattgcca atccagtcac actgccagta acaatcaatg ttgttcccca
     6901 acctgcaaga ccgagcatag ttaactcctc agtagaagtt gttttccagg actgttttgt
     6961 ggacaaacca aaagaagcgg tcttccagat actaaactcc ggcgacaatg atttgaagga
     7021 gggcataaaa ccaatacttt caacctcgtg gctttcaaac ccgcgctttg tcaaaaacaa
     7081 aggtaattca tggctataca tatgcacgat ctcaccttca acattctggc caaatcaaca
     7141 atatacagca tctatcaaca ccatgataag ctcgacgcca attacgctaa aaattactgt
     7201 aaaaacaatg cacatacccg aatgcaagat agaattttca ataggatcaa agcagtacat
     7261 tgtgaatgga agatctctac caatggatgc tccgccttat gtttccaaaa atggcagcac
     7321 tatggttcca atcagatata taggggaccc acttggttat ttctacaaag cacagacgga
     7381 atgggtaaaa gagctgaaaa cggtattgtt tacgctggct ggaacaacac taaggctcat
     7441 agtggactac cagaatgcca tagtggaaag ggccgatggc tccatagaca cggtaaaact
     7501 ggcttctccc ccagagataa aaaatggcag gacattcatc ccgccaagaa ttattggtga
     7561 aacgtttggt gcaaaaatat cttgggacga cagaaccaga aaagccaccc tcgtatttaa
     7621 ccaaccacca ccctgatcaa accaggaaaa taacgtctcc cagtctcatt agaactcacc
     7681 caaaaataac ttgaatgcaa cctaacattt catatattcg aaatcatgaa atatttggat
     7741 gaagaaaatg aaagagaact tcaggaaatt tcagacatat tctcggctct gtcttcccca
     7801 aagaggctca aaataatctc cttaatcgaa gatggaccct tatcagtcac tgaaatagcc
     7861 cagaaaatga agtgcaggcc accatgtgtt tcacaacacc tctcggttct caaatcatgc
     7921 aaaatagttg attgtgagag aactggtcat gaaatggtct acaaattggc actttcgtgc
     7981 gtcaaatcat tcatatcttg tctgctagga aaatgtgata gcaacgattc tgggtgttca
     8041 attgtaaaat cagtccatgc aaaataacaa aacaggagga aatgatgaaa atacaagtgc
     8101 taggaatggg atgcgcaaaa tgccactccc tcgaagcaaa tgtcagagaa gctgttgcaa
     8161 agatgggcat cgatgctgaa attgagccaa tcaaggacat acagaaaatc gtcgagatgg
     8221 gtgttatggc aacaccagca atcgcaattg atggaaaagt tgtctcttca ggaaggatgc
     8281 tctctccatc gcagataacg gagctcataa aacaaaacca ataatgaaga atacaacaaa
     8341 aattctcctt tccatacttg tgctcgtgat tgccattgtg aatacaaacg cgatgtttca
     8401 accaggaata cctgacaatg gtctggtagc aaacgatgat tgggcctcat ctccacagtc
     8461 gcaagtctgg gaaaaacagt tgcaaacaga tatcgtctcc ggacccgttt ttatcggctc
     8521 aagagtagct gtggcgcttg gaaactcgca agtaaatgct ttcagcatct attcaggaaa
     8581 gctggcctgg tcatacaagc ttgaccagaa accaaaccag atagcctcag gtagtgattc
     8641 ggtttttgtc aaaacagcca ggcaggtcac ttccattgga gatggcgggc aaaaaaactg
     8701 gatattccag acagaggcga acataactac ggatttggca atccttggca atatgctatt
     8761 ttttgggtgt gctgacggga atttctactg tgttaacacc aaaaatggca aaaaaatctg
     8821 gctatctgct ttcaagtcaa taccaaatat aggcccgaaa atgcttggtg aagattacct
     8881 gttttttgca tccacagaca aaaagctcta ttgcctcaac acatcgtcag gaaaacagaa
     8941 gtgggaactt gccctggatg actcgccaga atggattttt gccgaccaga acagggtact
     9001 tgcgcaagaa ggcaaatatc tttttgcaat cgaggcccaa agcggaaacc caagatggaa
     9061 atatgaaaca ggcactggct ctttacaggc ggtttcgtca aaaggcaagt tttttgtcac
     9121 gactccggct ttggcgatag attgctttga tgccaacttc tttggcaagg catgctgtat
     9181 atcgacaggc aaaagattgt ggctgtcaca actggattct ttcccgacaa caagattgtt
     9241 ctgttccgaa gaaacaattt ttgtcccaaa aggcaacttg attgaaaaaa tatctgtggc
     9301 tgatggctca aaacagggca atatcacgct tgacaagaag attgaatcga ttgacctgca
     9361 aggagacaac attgcaataa tctctgggaa cacgatttcc ctcttcaata aagtgccgga
     9421 aaaaatcaca ttcgtggttg gacaaaaaga actgggcaga gattcttttg ctgttgagat
     9481 ggacgttgca ccagtcataa ttgatggtaa atcattcctg ccagcaaggt atgtttgcga
     9541 accttttggc ggctcggtct catgggacca gggatcaaaa aagacaactt gccagctgaa
     9601 tggcaaaaca ttgagtctat gggtcgggaa aaataccgcc agaacaagcg gcggtgacaa
     9661 gcagatcgac agcaacccca aaataatccc attcatacaa aatggaagag taatgctacc
     9721 aatgagattt ttagcagaat cacttggatt aaaagtaggc tatgaaaaca aaaagataag
     9781 tattgaaaat gaaagaggcc aaaaatgaga agaaacattt tatcaagctt tgttttattg
     9841 gcggtttttg caggctccat ttttctagca tcatgtggaa gcaatgggaa tcttccttgt
     9901 gttagtgagg gggaaactgg taatggcacc atttcgatca aaaatgattc tggaatgcca
     9961 atggtggtca aggttggaag ggcaacctgc aaaccctgca tagtaatgac agacacagtc
    10021 aatgagatcg gcaaggagct tgaaggaaaa atccagatcg caattgtcga cattgacgaa
    10081 gaccctgaag cagtggagaa gtttcagata cagggtattc caacaacatt cgtatttgat
    10141 gcgtcaggca accagacttt caggaaaacc ggcatcgcaa caaaggatga gcttgtaagt
    10201 gagctcaaga aagcaggaat gcagtgaaaa acaatatcac aaacacaatc attcttgtca
    10261 tagtggccct tgccgcactg ggtcttctat cgtacaaact ctttttcgag aagggatccg
    10321 attttccaaa acctcaaaaa ggtgaggtca caatcgtcga gcttggggca gacctttgtg
    10381 aaccatgcac aaaactccgc cccataatgg aagacctgaa aaaagaactt gagggaaaag
    10441 ctgttctaaa cagccttctt atagataatt caatgaaatc tttctacaac gtgaaagtaa
    10501 tcccggtcat catcatcttc gacaaagaag caaatgaggt agctagaaga atattttcac
    10561 ttgaagaagt agatgatgcc agaaactgga taaaagagaa aactgaaccc ctgggggtgc
    10621 agtggtgatc gagtccctcc tccagtcaat acaaagcaac ccctggattg caccatttac
    10681 ggtgtttttc accgggtttc tcacggcttc caatccttgc gttcttggca tgatacccct
    10741 catgatcggg gcatcagggg cataccaggg cgaagacaag tcctactgga aagctgcaaa
    10801 gttctctctg ctatttgtcc tcggccagtc aatctctttc atggtgctgg ggataatcgc
    10861 agcactcatt ggtcagcagg caacgctgac cggcccctgg tggacatact tcatggcttt
    10921 cctctgcatt ctgatgggac taatgttcgc tgatatagtc aaattcaaca tcccagtgcc
    10981 aaagatactg acaaaaccaa gaacagggct cttcggggct tttatcctgg gcgttctttt
    11041 tggctttgtc tcaacaccct gtgctgtacc gattctttca gtattgctcg ctttgatagc
    11101 aacaggcgga aacacatatt ttggcggcct tctcctgctc tgctatgccc tcgggcattc
    11161 agctcttctt cttgtggcag gcatctcgat aggtgccgcg caggccttca taaacaatcg
    11221 gaacttgaga acaacttctc ttgttctcag aaaagtattt ggacttctaa tcatcggctt
    11281 tgggttctac ctgctctata tagcctaaat ttctggagga aaaatggaca tcttcgcacc
    11341 agtaaactgg tttgcaaaat ggctcacata cgacattttc agaatacagc acgaatcact
    11401 gcttggagaa gctgtcagtt tctttgtttt tgacaccata aaagtcttca tgcttctcat
    11461 cgcaatcata tttgtggttt cctacctgag aacatacctg ccaccagaga aagtccggtt
    11521 ctttatctca aagagaaaca aaataactgg acacattatg gctgcagcct tcggtatcat
    11581 aacgccattt tgcacctgca gtgcgatccc gctctttctg ggcttcctgg aagccggtgt
    11641 cccgcttggc gtgacattct cattcctcat agcctcgcca atgataaacg aggtggcact
    11701 ggtaatgctc cttggcatgt ttgggtggaa ggtggctctg atttacattg catcaggaat
    11761 gataattgct atcctgtccg gactcgtcat cggccagct
//
LOCUS       DAOSRI010000016        11705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5455_length_11705_cov_4.179056, whole genome shotgun sequence.
ACCESSION   DAOSRI010000016 DAOSRI010000000
VERSION     DAOSRI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5455_length_11705_cov_4.179056"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="cgtA"
                     /locus_tag="PK717_00935"
     CDS             complement(<1..777)
                     /gene="cgtA"
                     /locus_tag="PK717_00935"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOR46271.1"
                     /translation="MFVDEVTIKVAAGKGGDGVVTFRREKFVPRGGPSGGKGGHGGDV
                     YLVADSNLRDLSYLVDHVNWKAEDGVNGSSENCTGAAGDDIYIKVPVGVAIYLEDKDN
                     LIAELLEDKQEFLVAKGGRGGLGNANFANARRQAPHIATAGERGEKAKLIIELKILAD
                     VGLVGMPNAGKSTLLGAISRASPKVGDYPFTTLEPSLGVITTKDWNRFTVADIPGLIE
                     GASQGKGLGFEFLRHVERCRVLLYLIDLASDDPCATLEMLW"
     gene            complement(777..1037)
                     /gene="rpmA"
                     /locus_tag="PK717_00940"
     CDS             complement(777..1037)
                     /gene="rpmA"
                     /locus_tag="PK717_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOR46272.1"
                     /translation="MAHKKAGGSGHNGRDSNPKYRGVKVADGQEVLPGGVIIRQCGTH
                     FHPGEGTDIGRDYTIFAITQGVVKFQRVGKTGKRVVVLPKAN"
     gene            complement(1047..2129)
                     /locus_tag="PK717_00945"
     CDS             complement(1047..2129)
                     /locus_tag="PK717_00945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR46273.1"
                     /translation="MKIRNMPNTPFLTINTKKIEQNAKKMATLVHEHGARLWGVTKVV
                     LGDVAVAKSMLQGGCDGLADSRIESLRKLRQTFPRTPLLLIRPPSMSRLKELVQIDCV
                     SLQSDLETIKILGEVASSIGRTQKIILMVELGDLREGVTEEELSPYVFEVLRTPGIEL
                     EGLGAVLTCFAGVVPDQNTVSRFIELASKISSEYKVEIKYLAPAATNFVPMLMAGKLP
                     RAINDFRIGEGIILGMNVLDRTPLPGFDVDTVELSCEIIELKSKPSKPTGIVSQDAYG
                     SHPVFEDRGTRKRAIINIGRGDCIPELLAPLDEGVIVLGGSSDHVVCDVEDATGLSVG
                     DILRFRPGYGALLSLVTSPFVEKVFV"
     gene            2346..3698
                     /locus_tag="PK717_00950"
     CDS             2346..3698
                     /locus_tag="PK717_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOR46274.1"
                     /translation="MKSNDYVKLVLILLIVAFSALIAFFPTAFTKEATIVDSKGTPKT
                     ITGPAFPYRLGLDLKGGIHVVLDAIDTPEQPVTDEKIDRTIAVLESRINKLGLSETTV
                     QRQGKLNRIAIDLPGYQNKDEAMAILGKTALLQFKDADGKLIMTGDKVKDAQAVYGQA
                     GNSIGQEWHIVMTMTPEGQQAFTKATEEVVARTDEKSRYIAIYLDDELLMKPNVKEKI
                     DLDTVSITSGGGDPTEKQAWSQQYALLIAGGALPLKLSSDPAELRVVGASLGQDTVNN
                     VIIGAILAMILVGLYMLLYYKGMGLVSILALGIYVVIYLTILLLVGQVFSLPAIGGAI
                     ISIGMAVDSNVIIFERVRDEINKGKLPTSSVSQGFGKALGTVLDSNITTLIGAGVLYW
                     LGTGPVKGFAVTLALGIFASFFTAVFVTRFIMDMIIRFWKPEPGSPMFRFFYGNGGAA
                     "
     gene            3695..5113
                     /gene="secF"
                     /locus_tag="PK717_00955"
     CDS             3695..5113
                     /gene="secF"
                     /locus_tag="PK717_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOR46275.1"
                     /translation="MKFLNPIHWDIIGKLKWLVITFIAVIAFGILMIGFRWLTTGSSA
                     DVAYFMFEKAIPGQVTEIAKTVYQFEEVQKLSNFSIEVPAPDANYKEQVRLNIRADKL
                     TNDEVKKIGEKLSTEYGKLAKDPAIETISVPGQPFNWGLDFTGGTIVEMSFKNPFVDN
                     TGKALPDEEVISKVRTAFAKNGVSLTVVQVERLASEAEQGKDIANAILVRTQESSTDK
                     LNSVIAELTSTFGEEDVAKRKIDTIGPVVGQELKRSAIIALVIALSVVFIYIAFRFQA
                     RMGVAAIVCLIHDLLFVLGVLSLSWVEVNSAAVAALLAMITYDVQDTVVIMDRVRENT
                     KLYLGKIPYDKLINMSVTQTYMRSFNTQFVTFMCVVVLLFFGGKSIMDFTLIFLFGQI
                     VGTYSSIYIAAPMLVVWKNFEDRTSTKQPGKTYQLSTPEGIKIAVKETQPQINKPVVK
                     DNLAGKPPAKKGPPKKKSNKRR"
     gene            5144..5527
                     /locus_tag="PK717_00960"
     CDS             5144..5527
                     /locus_tag="PK717_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR46276.1"
                     /translation="MKKLILVAQETADARKGVLNALSPLYAEGYSILTCMSGEELIKK
                     MSLGQACLAVVDLTMPELNGFELINKLTSDPSTTNVPIMALTPPTLGVFKYITRCMGV
                     TYFFNRPTSPVKILEMARECMGRAE"
     gene            5524..6414
                     /locus_tag="PK717_00965"
     CDS             5524..6414
                     /locus_tag="PK717_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46277.1"
                     /translation="MNFIFERLPKGAKPKPLAQEFTSSPKDAIYTMRTSMFSGYLEGN
                     FVNSKLGIYFYKGRPIFTIYQSKDWFEFGQKAIDFATKDQGSSRNCYSTSDVDPDILK
                     CFCSLLSPYPIFRNFYPQMLVIDELFSRMVQEKFTGCIYISTLSDQVLLFVHDGMILE
                     WEEGKIINRYPIDESPVTKDMAKAVALMSDERAGIDIYEMPENLYSDLTSFEYGVTFK
                     TTKSLLILKDALSKIAGDELKGKSVDFDRALDENSADINNIEEFVTNLEKHLTVDISR
                     KILQGLTEKLMVEIAEFKKS"
     gene            complement(6401..7237)
                     /gene="ispE"
                     /locus_tag="PK717_00970"
     CDS             complement(6401..7237)
                     /gene="ispE"
                     /locus_tag="PK717_00970"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOR46278.1"
                     /translation="MKRSMSINSRDLPVSFRTRAYAKINWDLHVLARRPDGYHEIDSL
                     VARIDLYDEIDILISNGKGITVDCEVAPGKDNLVYKAASLLLESFNGNFSVEIKIKKN
                     IPHGAGLGGGSSDAAAVISCLGSFIGLDNTLLFEIAAKVGSDVPSFLFEGWRRMTGRG
                     EIVEPLKGFEEEILLVCPDCHVPTPRVYSNWDQKPVSTRPGSMSDRLKSPQNDLFEAA
                     NLVCPKIGEAINALLDHGANIASMSGSGSSIFALFKQKAALLGAALELGKKYTCHRIR
                     IF"
     gene            complement(7182..8006)
                     /gene="rsmA"
                     /locus_tag="PK717_00975"
     CDS             complement(7182..8006)
                     /gene="rsmA"
                     /locus_tag="PK717_00975"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOR46279.1"
                     /translation="MSSNGLKDLKDDLQKFEIRLKKSFGQNFLHDPVAINRIMDACNI
                     KHSDWVVEIGAGSGYLTSALAINAHQVTAIEIDTDLRPLLLKTTGLTSNIKLIFGDVL
                     EMDLSSLKPTVVVGNLPYYCSSAILRRWANQTPDVPAFFMLPEDVVKHLLASPGDPSY
                     TAFSVYAQYAFSFKKLFEVHPSSFFPRPNVGSAFVSFDQPKTPRLPKRAEDHFIRVVE
                     GAFMLRRKTMVNSLSEAGFDTDDVKSAMSFIGLEPNVRGETLNVDQFKRLAGFIPH"
     gene            complement(7972..8976)
                     /locus_tag="PK717_00980"
     CDS             complement(7972..8976)
                     /locus_tag="PK717_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOR46280.1"
                     /translation="MNCEDTKTAYWIIAVLGFVFVILGFASSKAVAVYIGQELHVGVT
                     FQKTISGALQEMGVKIDPSDRVVIKPAGFVVSNMDTTFRSGEKIEFEKANPVIIQFDG
                     MRKQILCTRKNLRNAVSKIGIKLGPLDRVQVSEDPYRPGAMLTRVVRVEHKLVKRKER
                     IPYEIDYVPNNQVHQGKVVVWKPGSGGEREDLYREIYEDGKLVTSTFVSTRASHRPIH
                     EEMAIGEAEMPGGAIEKILVEATAYSPTVAECDADPWTTATGMRSGYGIIAVDPTVIP
                     YYTKVYVEGYGYAIAGDCGGAIKNNIIDVFFYKPEEAMRWGRRKVNMYVLEWPKRSQR
                     "
     gene            8999..10414
                     /locus_tag="PK717_00985"
     CDS             8999..10414
                     /locus_tag="PK717_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR46281.1"
                     /translation="MNVILSKETMIRKARETLANHFRGIKLHDDAMMVGGTIRDILLG
                     RTPKDIDIATPKTAELVQGFVDKGFTHVLLDEQRDIHRMVGNGVHIDITPLHHDHIHD
                     LIGRDFTINAIGLVISTEEVLDPQGGMKDLSKSLVRALSEDRFLEDPLRLIRAFRIAA
                     DLSFSIDPVTKRFITKHAHLINRPSKERIRDEICWTIEAPRSYPVIEELTRTGILGRI
                     LPEFASVSQVIAHKPHALGLTEHLLWTYKYLEDILEDLDSMLGDYADNARELLSTVMA
                     SDRRMFICTKLAGLLHDIGKPQTITYEEEVHFLEHDRVGSEMVSKRMEELKFSSAETK
                     TVSGIVLNHMRPHNLQKLDSVTPKAVFKYFRDLEQIAIPTLLVAVADAYATQMMPYGS
                     LDAYHEFVKSMVAENQQMGKPKPLLTGDEIMKLLGIDPGPEVGLAVSYLAEAQALGQI
                     TTKTQAKELIKSKGYLNEGNA"
     gene            10398..11429
                     /locus_tag="PK717_00990"
     CDS             10398..11429
                     /locus_tag="PK717_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR46282.1"
                     /translation="MKETLEKFVRISSSSCHEEKVAELFQQTISETGYETRTDRMGNV
                     YAEIPCGVDGAKKIMITAHSDEPALAVQGFTEDGFVRIWDWSGVDHKVLPGQEVVIHG
                     TCEIPAIIGILPPHLTKPGDKKAVPITKLYADTGMPADWVTGKVKIGDQVTFKKRFSE
                     LKNGRVAGCALDNRLSVTMLLELAKKLKRVKLDVDVVLVCTTQEEIGAYGGFAAATEV
                     RPDLAIAMDTTIAEQHGESGKSYLPLDKVCLDNGPIINPKVRAAFLGSAEKLNIKTSE
                     YIFSSGSQTEADSAWEAGLGRPIAVVSIPVRYLHQPVETVDLSVAESGVDMMCEFLMG
                     LPASLEGVI"
     gene            11429..>11705
                     /locus_tag="PK717_00995"
     CDS             11429..>11705
                     /locus_tag="PK717_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOR46283.1"
                     /translation="MELLKKLCNASGAPGDESEIRQVLTDFYSQMGIAPTTDHVGNIF
                     FVKEGKPGKPIIMLDAHMDEVALQVIGHTSEGELKIAQLGGIDDRVLA"
BASE COUNT         3345 a         2790 c         2575 g         2995 t
ORIGIN      
        1 ccagagcatc tccagtgttg cacatggatc atccgaggcc aggtcgataa gataaagcaa
       61 aactctgcat ctttcaacat gacgcaaaaa ttcaaaacca agccccttgc cctgcgatgc
      121 accctcaatg agacctggaa tatcggcgac agtaaatctg ttccagtctt tggttgttat
      181 gaccccaagc gacggttcaa gagtagtgaa aggatagtcg ccaacttttg gcgaagctct
      241 tgaaatcgca ccaagcaatg ttgatttgcc ggcattgggc attccgacaa gtcctacgtc
      301 ggcaagtatt ttcagctcga tgatgagctt ggctttttcg cctctctcgc cggccgtggc
      361 aatgtgcggc gcttgtctcc ttgcattggc aaaattggcg tttccaagac cacctcttcc
      421 gccttttgca acaagaaact cctgcttgtc ttccagaagc tctgcgatca ggttgtcttt
      481 gtcttccaga taaatggcaa caccgactgg aactttaatg taaatatcat caccagcagc
      541 accggtgcaa ttttccgaag aaccatttac gccatcttca gctttccagt ttacatggtc
      601 gacaagatag gaaaggtccc tgaggtttga atctgcaaca agatatacgt cacccccatg
      661 accgcccttc cctccggaag ggccgcctct ggggacaaat ttctctcgtc tgaaagtaac
      721 gacaccatcg ccaccttttc cagcagcgac cttaatcgtc acttcatcta caaacatcag
      781 ttggcttttg gaagaaccac cacacgcttg ccggtttttc cgactctctg gaacttgact
      841 actccttgtg tgattgcaaa gattgtgtag tctcttccaa tgtcggtgcc ttcacctggg
      901 tgaaaatggg tcccgcactg cctgatgatt actccacctg gtaaaacttc ctgcccgtcg
      961 gcgactttta cgcccctgta tttcgggttg gaatcccttc cgttatgacc ggatccacct
     1021 gcttttttat gtgccatgat atcctcctat acaaaaactt tttcaacaaa aggcgaagtt
     1081 acaagcgaca aaagtgcacc gtaaccaggc ctgaacctga gtatgtctcc aacactcaga
     1141 cctgttgcat cttcaacatc acatacgaca tggtccgaag aaccaccaag aacaatgacg
     1201 ccctcgtcaa gcggcgcgag gagctccggg atgcaatctc ctcttccaat attgatgatg
     1261 gcgcgttttc ttgttcctct atcctcaaaa accgggtgtg agccgtacgc atcctgcgag
     1321 acaatccctg tgggtttcga tggtttggat ttgagttcaa ttatctcgca tgaaagttct
     1381 acagtatcaa catcaaaacc gggtagcggt gtcctgtcaa ggacattcat acccaaaata
     1441 atcccctcgc caatcctgaa atcatttata gccctgggga gcttgccagc cattagcatc
     1501 ggaacgaaat ttgttgctgc aggagcaaga tactttatct ccaccttgta ctcggaagat
     1561 atttttgaag cgagctcaat gaaccttgag acggtgtttt gatctgggac aacacctgca
     1621 aagcacgtca ataccgctcc caatccttca agttcaatcc caggcgtcct tagcacttca
     1681 aaaacataag gagaaagctc ttcttctgta acaccttcgc gcaaatcgcc aagctcaacc
     1741 atcaaaatga tcttttgggt cctgccaatg gatgatgcaa cttcaccaag gatttttatc
     1801 gtttcaaggt cactctggag actgacacaa tcaatctgta caagctcttt aagtctgctc
     1861 atcgaaggcg gacgaataag aagaagtggg gtcctgggga aagtttgtct aagcttcctg
     1921 agactctcga tcctggaatc agccaaacca tcacaccccc cctggagcat tgattttgca
     1981 acagcaacgt cacccaaaac gactttggta acgccccaaa gcctggcccc gtgctcatga
     2041 acaagggtcg ccatcttttt ggcattctgc tcaatctttt tagtgtttat agttaaaaat
     2101 ggagtgtttg gcatattccg aattttcaat aatacaatat agttcaatcc agtcaataaa
     2161 gtctcgttca tagctgattt atccttgaca atcagaaaca ccttccacta atatagttag
     2221 gttgtgtaac catatttatc tgacctggca aggttttatc gatggttgga aaatattgga
     2281 aatcgaaata aagggcttat taagcccctt aggtctaatg gctaaatatg ctaatgaggt
     2341 gagtgatgaa aagtaacgac tatgtaaagc tagttttgat tttgttgatt gtagctttct
     2401 cggcgttgat cgcatttttc ccgacagcct tcacaaaaga agctacgatc gttgacagca
     2461 agggaacccc caagacaata accggccccg ccttccctta caggctcggc ctggatctca
     2521 agggtggaat ccatgttgtg cttgacgcta ttgatacgcc agaacaacca gtcacggatg
     2581 agaagattga tcgtacaatt gctgttctcg aaagccgtat caacaaactt ggtctttcag
     2641 aaacaactgt ccagcgtcaa ggcaaactta accgtatagc gatcgacctg ccaggctacc
     2701 agaacaagga cgaggcaatg gcaatccttg gcaaaaccgc tcttctccag ttcaaggatg
     2761 ctgatggaaa gcttataatg acgggtgata aggtcaagga tgcacaggca gtttacggac
     2821 aggctggtaa tagtataggt caggaatggc acatcgttat gaccatgacc ccagaaggac
     2881 agcaagcatt tacaaaagca acggaagagg tagttgcaag aactgatgaa aaatctcgtt
     2941 atattgcaat ttatctcgac gatgagctcc ttatgaaacc aaacgttaaa gagaagattg
     3001 atctagatac tgtctcaatt acctcaggtg gtggtgaccc aacagaaaaa caggcctggt
     3061 cacagcagta cgcactcctt atcgctggtg gcgcacttcc gctcaaattg agctcggatc
     3121 cggcagaact tcgcgttgtt ggtgcttcac ttggtcaaga cacggtgaac aatgtcatca
     3181 ttggggcaat actcgcaatg atcctcgttg gactctacat gcttctctac tacaaaggaa
     3241 tgggtcttgt ttcaattctt gcccttggaa tatacgtagt aatttacctc accatccttc
     3301 tgctcgttgg tcaagttttc tctctgccag caatcggtgg tgcaattatt tccattggta
     3361 tggctgttga ctccaacgtc atcatctttg aacgtgtcag agatgaaatc aataagggca
     3421 aattgccaac ctcttcagta tcacagggat ttggcaaggc cctcggtact gttcttgact
     3481 ccaacatcac aactctcatc ggtgctggcg ttctttactg gcttggaacc ggcccagtca
     3541 aaggttttgc agtaacactt gcacttggaa tcttcgcctc attcttcaca gcagtgttcg
     3601 tgacaagatt tatcatggac atgataataa ggttctggaa accagagcca ggctcgccaa
     3661 tgtttaggtt cttctatggc aatggaggtg ctgcatgaaa tttctcaatc caatacactg
     3721 ggatattatc gggaaactca aatggcttgt tataactttt atagcagtca tcgctttcgg
     3781 aatactgatg atcggattca gatggctcac aaccggctcc agtgcagatg ttgcctactt
     3841 tatgtttgaa aaggcaatcc ctggccaggt gactgaaatc gcaaagacag tctatcagtt
     3901 cgaagaagtt cagaaacttt caaacttctc aattgaagtc ccagcaccag atgccaacta
     3961 caaagagcag gtcaggctaa acattagggc tgacaagctc accaatgatg aagtaaaaaa
     4021 gattggcgaa aaactttcta cagaatatgg aaaacttgca aaggacccgg ccattgaaac
     4081 catttctgtg ccaggacaac cattcaactg gggtcttgat ttcaccggtg gaacaatcgt
     4141 tgagatgagt ttcaagaatc catttgtcga caacaccggc aaagcacttc cagatgaaga
     4201 agtaatcagc aaggttagaa cagcttttgc aaaaaatggc gtctcgctca cagttgttca
     4261 ggttgaacgt ctggcctcag aagctgaaca gggcaaagat attgccaatg caatcctcgt
     4321 ccgtacacaa gaatcatcca ctgacaaact aaactcagta atagcagaac tcacctcaac
     4381 atttggtgaa gaagacgtag caaagagaaa gatcgacaca ataggaccgg ttgtcggaca
     4441 ggaactcaaa aggtccgcca tcatagcact ggttatcgct ctttcagtag tcttcatata
     4501 tatcgcattc agattccagg ccagaatggg tgttgccgca atcgtgtgtc tgatccacga
     4561 ccttttgttt gttcttggcg tcctctccct gtcatgggta gaagtaaatt cagcggcggt
     4621 ggcagccttg ctcgcaatga taacgtatga cgttcaggac actgtcgtta ttatggatcg
     4681 tgtcagagag aatacaaagc tctatcttgg caagatacct tacgacaagc tcatcaacat
     4741 gtctgtgacc cagacctaca tgaggtcctt caatacacag tttgtcactt ttatgtgtgt
     4801 cgttgtcctg cttttctttg gtggaaaatc catcatggat ttcaccctca tcttcctctt
     4861 cggacagatc gttggaacat acagctcaat ttacattgct gccccgatgc ttgttgtctg
     4921 gaaaaacttt gaagacagaa ccagcacaaa acagcctgga aaaacctatc aattatcaac
     4981 tccagaaggc attaagatag cagtaaagga aacacagcca cagattaaca agccagtagt
     5041 taaagataat cttgctggta agccgccggc caaaaaaggc ccgccaaaga aaaaatccaa
     5101 caaaagacgt taggatttga aaatactatt cagggaggcc tgagtgaaaa agctgatcct
     5161 tgttgctcaa gaaacagctg atgcgagaaa gggcgtgtta aacgcccttt ctccattata
     5221 tgcagaagga tattcaatcc ttacatgcat gagtggtgaa gagctcatca agaagatgtc
     5281 tctcgggcag gcctgtctgg ccgtagtcga cctgacaatg cctgaactga acgggtttga
     5341 actcatcaac aaactcacct cggatccttc tacgacaaat gtcccaataa tggcactcac
     5401 cccaccaaca ctgggagttt tcaaatatat cacaaggtgc atgggcgtta cctatttctt
     5461 caacaggccc acatcaccag tcaaaatcct cgaaatggca agggaatgta tggggagggc
     5521 agaatgaatt tcatatttga gagactgcca aaaggagcaa aaccaaaacc actggcccaa
     5581 gaattcacat cttccccaaa agacgccatt tatacaatga gaacatccat gttctcgggt
     5641 tatcttgagg gtaattttgt aaactcaaaa ttaggcatct acttttacaa gggaaggccc
     5701 atattcacca tataccagtc caaagactgg ttcgaatttg ggcagaaagc catagatttt
     5761 gccacaaagg accagggatc atccagaaat tgctactcaa caagcgacgt tgatccggat
     5821 atcctgaaat gtttttgctc gttgctttcc ccatatccaa tattcaggaa tttctaccca
     5881 cagatgcttg tcattgacga acttttctca agaatggtgc aggagaaatt caccgggtgt
     5941 atatatattt caacactgtc tgaccaagtt ctgctttttg ttcatgatgg catgatcctt
     6001 gagtgggaag agggcaagat aatcaaccgc tacccgatcg atgaatcccc ggtaacaaaa
     6061 gacatggcaa aagcagtcgc cctgatgagc gatgaaaggg caggcattga tatctatgaa
     6121 atgcctgaaa atctttattc agacctgact tcttttgagt atggagtaac attcaagaca
     6181 accaaaagtt tgctgatact caaggatgct ttgagcaaaa tcgcaggtga cgaacttaaa
     6241 ggcaagagcg tcgatttcga cagagcgctt gacgaaaact cagctgatat aaacaacatt
     6301 gaagagtttg tcaccaatct ggaaaaacat cttacagtcg atatttcaag aaaaatcctt
     6361 cagggattga ccgaaaaatt gatggttgag atagcagagt tcaaaaaatc ctgattctgt
     6421 ggcaagtata ttttttacca agctccaaag ccgctcccaa aagggcggct ttttgtttga
     6481 aaagggcaaa aatcgaactg cctgagccac tcatgctggc aatatttgca ccgtgatcca
     6541 gaagtgcatt tattgcctcg ccaattttgg gacataccaa attagccgct tcaaaaagat
     6601 cattttgtgg tgatttcaac cgatctgaca tactgcctgg tcttgttgaa acaggtttct
     6661 gatcccaatt tgaatacacc cgtggtgtgg gaacatgaca atcaggacaa acaagcagta
     6721 tctcttcttc aaaacccttc aaaggttcaa cgatttcccc cctgccagtc atccttctcc
     6781 atccctcaaa cagaaacgat ggtacatcag aaccaacctt ggcggcaatt tcaaacaaaa
     6841 gggtattgtc caagccaata aaagaaccaa gacaggaaat tactgcagct gcgtctgatg
     6901 agccaccacc aagccctgca ccatgtggta tgttcttctt tatttttatc tcaacagaaa
     6961 aattcccatt aaaggactcc agcaagagag atgcggcttt gtataccaga ttgtcttttc
     7021 ctggcgccac ttcgcagtct acagtgatcc ccttgccatt acttatcaaa atgtctattt
     7081 catcataaag atctattctg gcaacaaggg aatctatttc atggtaacca tccggccttc
     7141 tggccagaac atgcaggtcc cagtttattt ttgcgtatgc cctagtgcgg aatgaaaccg
     7201 gcaagtctct tgaattgatc gacattgagc gtttcacctc tcacattggg ttccagcccg
     7261 atgaatgaca ttgcgctttt cacatcatct gtatcaaacc ccgcttcaga aagtgaattg
     7321 accattgttt ttcttctaag catgaaggca ccttcgacca ctctgatgaa atgatcctcg
     7381 gcccgttttg gtaatcttgg tgtcttcggt tggtcgaatg aaacaaaagc agaaccgaca
     7441 ttcggccttg gaaaaaatga tgatgggtgc acttcaaaaa gttttttgaa tgaaaaagca
     7501 tattgggcat aaaccgaaaa agctgtgtat gatggatctc ccggcgatgc cagcaggtgt
     7561 ttgacaacat cctctggtag cataaagaaa gcgggtacat ctggcgtctg gttcgcccat
     7621 ctccgaagta ttgcagaaga gcagtaatag gggagatttc caacgacaac tgtcggtttg
     7681 agtgatgaca ggtccatctc gaggacatcg ccaaaaatga gctttatatt tgaagtaagg
     7741 ccggtcgttt tgagcaagag tgggcgaaga tcagtgtcta tttcaatggc agttacctgg
     7801 tgggcattta tagccaaagc tgaagtaaga taaccagacc ccgcaccaat ctctactacc
     7861 cagtcggaat gtttgatatt acaggcatcc atgatcctgt ttatcgcaac aggatcatga
     7921 agaaagttct ggccaaagct tttcttgagc cttatctcga acttttgaag atcatctttg
     7981 agatctttta ggccattcga ggacatacat gtttaccttt cttctgcccc atctcatagc
     8041 ctcttcaggc ttgtagaaga agacatcaat aatgttgttt tttattgcgc caccgcaatc
     8101 gccggcaatg gcatacccat aaccttccac atacaccttc gtataatagg gtatgactgt
     8161 tggatcaacc gcaataattc cataacctga tctcattccg gttgctgttg tccatggatc
     8221 ggcatcgcat tctgcaacag ttggagaata ggcagtagct tctacaagaa tcttctcgat
     8281 tgccccacca ggcatctcgg cctctcctat tgccatttct tcatggattg gtctgtggga
     8341 ggctcttgtt gaaacaaaag tggaggtaac aagtttccca tcctcgtaaa tctccctgta
     8401 aagatcttct ctttctccgc ctgaccctgg tttccacacc acaaccttgc cctggtggac
     8461 ctgattgttc gggacataat caatctcgta aggtattctt tctttccttt ttacaagttt
     8521 gtgttccact ctcaccactc ttgtaagcat tgctcctggc ctatatgggt cttctgatac
     8581 ctgtacccta tccagcgggc caagttttat gccaatcttc gaaacggcat tccgcaggtt
     8641 cttgcgtgtg caaagtattt gcttgcgcat gccatcaaac tggataataa ctgggtttgc
     8701 cttttcaaac tctatttttt cgccagatct gaatgtggtg tccatgtttg aaacaacaaa
     8761 ccccgctggt tttattacga ctctgtctga ggggtcaatc ttaaccccca tctcttgcag
     8821 ggcgccactt atagtttttt gaaaagtgac tcctacatgg agttcctggc cgatataaac
     8881 cgctaccgct tttgagctgg caaatcctaa aatgacaaaa acaaagccca gtacggcaat
     8941 aatccaataa gctgttttgg tgtcttcaca attcatacat tttatgtagt atatacttgt
     9001 gaacgtaatt ctgtcaaagg aaacaatgat aagaaaggcc agggaaaccc tggccaatca
     9061 ttttcggggt ataaagctcc acgatgatgc aatgatggtt ggcggaacca tcagggacat
     9121 cctccttgga aggacgccaa aagacatcga cattgcaacc ccgaaaactg ccgaacttgt
     9181 ccagggtttt gtcgacaagg gattcaccca tgtcctgctg gatgaacagc gcgacatcca
     9241 caggatggtt gggaatggcg tgcacatcga catcacccca cttcaccatg accatatcca
     9301 tgacctgatt gggagagact tcaccatcaa cgctattggg cttgttataa gcacagagga
     9361 agttctggac ccgcaaggtg gaatgaaaga tctttccaag agcctcgtca gggcgctttc
     9421 agaagacaga tttcttgaag acccactcag gctgataagg gcattcagga tagccgcaga
     9481 cctctccttc tccatagatc ctgtcacaaa acgcttcatc acaaaacacg cccatctgat
     9541 aaacaggcca agcaaggaaa ggataaggga cgagatttgc tggacaatcg aggccccaag
     9601 gtcatacccg gtcatagagg aacttacaag gacgggcatt ctaggaagaa tactgccaga
     9661 atttgcttcc gtgtcgcagg taatagccca caaaccacat gcgcttggcc tgaccgagca
     9721 ccttctctgg acatataaat accttgaaga catcctggaa gatctcgact caatgcttgg
     9781 cgattatgcc gacaacgcaa gagaattgct gtcaacagtg atggcctccg acagaagaat
     9841 gttcatttgc acaaaattgg caggtctcct tcatgacata ggaaaacccc aaacaataac
     9901 ctacgaggaa gaagttcatt tccttgaaca tgacagggtt ggttctgaaa tggtctcgaa
     9961 gaggatggaa gaattgaagt tctcatccgc agaaaccaaa acagtttccg gaatagtttt
    10021 aaaccacatg aggccacaca acctccagaa gctggatagc gtaactccca aggcggtctt
    10081 caagtatttc agggaccttg agcagatagc cataccaact cttctggttg ccgtggcaga
    10141 tgcctatgca acccagatga tgccatacgg gtctttggac gcatatcatg aatttgtcaa
    10201 atcaatggtt gccgaaaacc agcaaatggg caaaccaaaa ccactcctca ctggagatga
    10261 aataatgaag ctccttggaa ttgaccctgg accagaggtg ggactcgctg tcagttatct
    10321 tgccgaggcg caggcactcg gtcagataac aacaaaaaca caggccaaag agctaataaa
    10381 atcgaaaggc tacctaaatg aaggaaacgc ttgaaaaatt cgtccggata agctcttcat
    10441 catgccacga ggagaaagtg gcagagcttt tccagcagac aatatctgaa acaggctatg
    10501 aaaccagaac cgacagaatg gggaatgttt atgcagaaat cccctgtggt gttgatggag
    10561 caaagaaaat catgatcacc gcccacagcg acgagcctgc gctggcagtc caggggttta
    10621 cagaagacgg ttttgtaaga atatgggact ggtcgggggt tgaccataag gtgctaccag
    10681 gacaggaagt tgtaatacac ggcacttgcg aaattccagc cataatagga atcctacctc
    10741 cacacctgac aaaaccgggt gacaaaaaag ctgtcccaat aacaaaactc tacgccgaca
    10801 ccggcatgcc agcagactgg gttaccggaa aggtaaaaat cggcgaccag gtgacattca
    10861 agaaacgctt ttccgagctc aagaatggcc gtgttgcagg ttgtgcgctc gataatagat
    10921 taagtgtcac aatgctgctt gaactggcca aaaagctcaa gagggtgaaa ctggatgtcg
    10981 atgtcgtcct ggtgtgcacc acacaggaag aaatcggtgc gtacggaggc tttgctgctg
    11041 caactgaagt aaggcctgat cttgcaatag caatggacac tacaatcgcc gaacagcatg
    11101 gagaatctgg caagtcatat ctgccgcttg acaaggtctg cttggacaat gggccgataa
    11161 taaatccgaa agtcagagcg gcgtttttgg gttcggctga gaaattaaac ataaaaacat
    11221 ctgagtacat attctcttcg ggtagccaaa ccgaagctga ttcagcatgg gaagcggggc
    11281 tcggaaggcc aatagctgtt gtctcgatac cggtcaggta cctccaccag ccagttgaga
    11341 ctgttgacct ttcggttgca gaaagcggcg ttgacatgat gtgcgagttt ctgatggggt
    11401 tgccggcaag cctggaaggg gtgatctaat ggaactcctg aaaaaacttt gcaatgcttc
    11461 cggtgcaccc ggcgacgaga gtgaaataag acaagtattg acggatttct attcccaaat
    11521 gggcattgct ccaacaacag accatgttgg caacattttc tttgtaaaag aagggaaacc
    11581 aggcaagcca ataatcatgc ttgatgccca catggacgag gtggccctgc aggtcatagg
    11641 ccatacctct gaaggcgagc tgaaaattgc acagcttggc gggattgacg acagggtgct
    11701 ggcat
//
LOCUS       DAOSRI010000017        11375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5630_length_11375_cov_4.327827, whole genome shotgun sequence.
ACCESSION   DAOSRI010000017 DAOSRI010000000
VERSION     DAOSRI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_11375_cov_4.327827"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="PK717_01000"
     CDS             complement(<1..2643)
                     /locus_tag="PK717_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46284.1"
                     /translation="MRRNLFIWLTVLAMTSSIFTPFSFSNTYSLTPTFSLEVTPGCGY
                     NVLKWEKVAGAINYWIYRGPSEGAEFDTPLTDFPIPDNVFKDEINIINGTKYCYFVRA
                     VDANANEFTQSKEACGTPTCENPPEEIPPENEDCRLMLEYQIGNKYYKSGDVQKGPME
                     AAPILTQNRMFLLIRYVAEEIGATIAYDASTKTVAIKTLDGQTLEIQIGNPTAKVNGN
                     PVQIDPNNPQVVAFIVGGRTVLPMRFVAENLGATGPEDIKWFEATKIVQLLFGDPNCR
                     RCMCVTVSKVDCNAKPPVVYGKDANGKEWRIYLSKELCLKVKEGGCYTFCGSIRQGAS
                     TPTTATALNTYFIATVVKQADCPCKPEPYPDYKCMCVTIETQNCSENPPVVYGVDEKN
                     QKWILQVDASLCQQMTPGTCWKVCGTVGVTTANTISASFPPRMKVTRAVKVDCPCGGN
                     QAPRCLCMKIEKVDCASAPPMAWGTDKNGKPWVMELDEKMCLELKLGSCYIICGHVQP
                     QSTATTYVQAKRIKVVRFEKIECEQCKPDEKCFCVKIDRVDCNATQPTVYGYDENGKL
                     WALYVDSKWCADMQPGTCWMVCGNPPEIIRVDSPPWMKVIRAEKVDCSKCKIKEQKEN
                     CICLTIERVDCNSDKPMLYGKDENGKFFSLLLSKELCEKYSNYLVPGKCLKVCYIITD
                     STLAVIMKVTSIAPVECPCEAQKEKCLCITLERVDCTSENPTALGKDESGQTRWSLGL
                     SKELCAKYANYLVPGKCLKVCGVPKPSPLSSALYMEVTSITPVECPCKEETKLDCFCV
                     KIEKKDCENLLLYATIDGRTLILSVSAELCKMAEPGTCWWVCGRKVEVAGAVPYFKVE
                     KYEKRDCPCGTEQPKEECFCVTIDR"
     gene            3056..6364
                     /locus_tag="PK717_01005"
     CDS             3056..6364
                     /locus_tag="PK717_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46285.1"
                     /translation="MRRNFVFSLLVAVLVFSIFFVNMPHIFALLGPIHVELNIMGDSP
                     SSVRAGQATAFKIHIRFNVNIRVHDWVKVWFPIDEASCKLEDICDGMPKITGAKEHPR
                     FVPNDEYFKKYNNEEEAKIGKLYEALDDRRGKTSFEKCECEDGAPPYSNCAPEGNCRI
                     IADPSGLGCWMTGMVMPALPRDEKKRYELMHSIIHEARLGYYDDCYCSGIPIIINTYK
                     ERSLQINSPLEVEAWRKGYNPIDYNIGKGAGILAPMTPGRYRLSVATAPEPTPVESEY
                     FVFPCSQISRPVVTLAGSDPGEPSDIAIKFSVGDGGALDKGKSKIWVRFPESFVFPEA
                     INARHVKINGDTLEASPKIDSQSHAIEITCPVNINNAGEVNIAFSPEAGIRNPPKETE
                     ATLTVWTDPEPEKVESLPFVVETKPRVEVKPNIFSNIAEYYAVALLPDGPIKAGKEFK
                     IIFPDGTLLPETIASSSIVLNGRKYDGKTSIGWQTVSLRSYFDIVDALKIRFLKTAGI
                     RNPLPGEYELSYEVDGKGYAFPKYIISDRHEVLDLMLSDYQGCERSGYKFTYIPSYRG
                     DLEPGDKLTVEFPWDTSIPKVIPNDAVLVGDKPSRSVSVDGLKLTITTDTAIKTADGG
                     AVVYITWDAGIINTRYSGKYNLKIWTEKDEPVFSPEFAITMPKVESWLEFKDPAQPDG
                     LEYGGCLWYKTPPILSITSCNPLAKIFMWYDNKTDSIRQYTGEKRLDYGLFRATIWYY
                     AQVGDIKEEPKFMHLCLDTVMPRFSIAKPDKQVVIANKRTYVVKGEREPCEMLTDGDA
                     EKYQAVDGIYVNGKELLAPEIFETVSRDEIKMTFETTIDLKEGSNVVEIRGIDQAGNE
                     KIEKRTIILDTIPPTIEIVRPDPKELQKPGENIAIQVKSETDASVFIDGQIAQKIEEL
                     PDGKTAIFEGVWDVVEGENKVKIFSTDVAGNTAVAYLTFKGGTKAVTIELWIGKTDFV
                     VNGKKMPNLATAPTASSPPLPKAFAGTTFMPIADVAKALGAEVNWDAKTKMIALTQTL
                     DNGTKKVIQLWIGKKQAKIDGKEVWIDAKKVLYPTIISGKTLLPLRFIAENLNCDVQY
                     DAKTKKITLTHPKP"
     gene            complement(6421..6864)
                     /locus_tag="PK717_01010"
     CDS             complement(6421..6864)
                     /locus_tag="PK717_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46286.1"
                     /translation="MNLKTLCCILVATSALVLVSCGGSGKITWNDNEQKVYDSFVQIE
                     QKTSEIIDSITKNNGKKPSELSKPIWDWTEYKNSQLSETYKEWRMSAGKAIEDKAMIM
                     RSTTADPQHETLKGKMQFETEYMAKMKSKIIQMVEKLKKGEDPGK"
     gene            complement(6869..7357)
                     /locus_tag="PK717_01015"
     CDS             complement(6869..7357)
                     /locus_tag="PK717_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46287.1"
                     /translation="MKPRFGWAINALLEGSLLYVFTTACFAVFGGNIGVVVSKVSFLA
                     GWLVFLGLFLILGFIFGKKLHENQILSIFGFATPTKSSFMTTLTVSAGLGLVSWALTW
                     ACHELANLITGQTVVGAIFNQTGPILLCLGGFAISGALVWTKNMLFENSPTPRVGNAR
                     RK"
     gene            complement(7531..8286)
                     /locus_tag="PK717_01020"
     CDS             complement(7531..8286)
                     /locus_tag="PK717_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOR46288.1"
                     /translation="MKKTIIMTTGIVIGSLIIGSIAAFGVVGAKDTQNVQEAENKNTI
                     FVTGVGTVTAKPDKFIIDLTITTTDKSARQAQQQNDSKANALIEALKKIGCKEDDLET
                     IGYYLNPVYKYFPEDNESRLVGYQASYTLKVTAYDLKKIGEIIDKGTENGVNQIGSIN
                     YTVKNEADFYAEALKTAMRDAKSKADTALGEYSLKVLSVKNINIAGYQPYITNKSMAP
                     SEDSRGGGAGGVSPNIISGNYSITVNVNVEFNY"
     gene            complement(8482..9291)
                     /locus_tag="PK717_01025"
     CDS             complement(8482..9291)
                     /locus_tag="PK717_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HOR46289.1"
                     /translation="MAKKAEEIGVKKANQSFTKTFTLSLLAGAYIAIGGFFSSTVLVG
                     SEAIPYGISKMLAGFAFCTGLILVVVGGAELFTGNNLIAMAWANKKITFSKLLRNWAI
                     VYFGNFVGSVIIAATIFLGGQYLFSGGQVGLWALKIAEGKFALQPSQMFFLGILCNVL
                     VCLAVWLTYSAKTTTCKIMAIIFPITAFVAAGFEHSVANMYFGTIAILIKNFAGQSFW
                     SSIGKTAQDFSHLSPGAMANNLLWVTLGNIVGGALFVGISYWFIYLREKTK"
     gene            9571..10053
                     /locus_tag="PK717_01030"
     CDS             9571..10053
                     /locus_tag="PK717_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46290.1"
                     /translation="MSLSTAYNILLELSSRGLLKKIYEPDGKKGRAKILFMPTEDGTL
                     TASRENGGNLMDCILACHFAIMTAVMKAPEILSSTRCCKDSSKSVVVGLMTGIWDKII
                     ENISCDQTSMEFLNRVSGTKRQFLKSISKLSDVEIRSLCRALTSFFFMANNLIIMGRG
                     "
     gene            10062..>11375
                     /locus_tag="PK717_01035"
     CDS             10062..>11375
                     /locus_tag="PK717_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR46291.1"
                     /translation="MNAIENAKRIAGNVVVNAAMNYITNGNVERKIDDLFNIALKLAW
                     RPCDREPILQLKSRFRDPDCPQKKLLVNVLKSDPNWLKKAAANFFVNSIIVGRKKTLE
                     LEKRNKMMLPFFLLIDPTERCNYRCKGCWAGNFDPCDMPYEVFDRLLCEAKELGIYFV
                     TISGGEPFLYKDLFKVMEKHPDMTFHFYTNGSLLADEEFADKFVALGNGLPCFSLEGF
                     EERTDERRGKGAFVKVMKAMDNLRERRYPFGISCTATSQNYKEITSDEFVDAMVKKGV
                     VLGWYFMYMPIGRDPDFEIMLKPEQRKYMWQRTTKIRNEKPIVFADFWNDGPVTDGCL
                     AGGRRYVHITADCHVEPCAFVHFNRPEDSVREKSLLKVLEESKLFNAMRARQNPAYES
                     NPMRPCWIVDRPWALRDVVKEVSADASEAGSIHLMDEKVAGELDRR"
BASE COUNT         2983 a         2520 c         2677 g         3195 t
ORIGIN      
        1 gcggtcaatc gttacacaga agcactcttc ttttggttgc tccgttccac aagggcagtc
       61 cctcttttcg tatttttcaa ccttgaaata tgggacagca ccagcaacct cgacctttct
      121 tccgcagacc caccagcatg ttccgggctc tgccatcttg cacagttcgg ctgataccga
      181 aagtatcagg gtcctgccat cgattgttgc ataaagaagt agattctcgc aatctttctt
      241 ttcgatcttt acgcagaaac agtcaagctt tgtttcttcc ttgcatgggc attccaccgg
      301 ggtgattgag gtgacttcca tatatagcgc agaacttaat gggcttggct ttggcacccc
      361 acagaccttt aaacacttgc ctgggacaag atagttggca tacttggcac agagttcctt
      421 tgagagacca agcgaccacc gtgtttgtcc actctcgtct ttcccaagtg ccgtcgggtt
      481 ctcacttgtg cagtctaccc tttccagcgt tatgcacaaa catttctctt tttgcgcttc
      541 acaggggcac tcgacaggcg ctattgatgt gaccttcatt atgactgcaa gcgttgaatc
      601 cgtgattatg tagcaaactt tcaagcactt tccagggacc agatagtttg aatatttttc
      661 gcaaagctct ttcgagagaa ggagcgagaa aaattttccg ttctcgtctt tgccataaag
      721 cattggtttg tccgaattgc agtcaaccct ctctatcgtc aggcaaatgc aattttcctt
      781 ttgttccttg atcttgcact tggaacaatc gaccttttct gcccttatga ctttcatcca
      841 gggcgggctg tcgacgcgga tgatttctgg cggatttcca cacaccatcc agcaggttcc
      901 aggctgcatg tcggcgcacc attttgaatc aacgtagagt gcccaaagct tgccattttc
      961 atcatagcca taaacggttg gctgggttgc attgcaatcc acgcggtcaa tctttacgca
     1021 gaagcatttt tcatctggtt tgcactgctc gcactcgatt ttttcaaatc ttacaacctt
     1081 gatcctcttc gcctgtacat atgttgttgc tgtggattgt ggttggacat gcccgcagat
     1141 tatgtagcag gaacctagtt tcagctccag gcacatcttc tcgtccagtt ccatcaccca
     1201 cggtttgccg tttttgtcgg tgccccatgc cattggcggt gcgcttgcac agtcgacctt
     1261 ttcgatcttc atgcagagac accttggtgc ctggttgcca ccgcatgggc aatcgacttt
     1321 taccgccctt gtaaccttca ttcttggcgg gaaggatgca gaaatggtat ttgcagttgt
     1381 caccccaaca gtcccgcaaa ctttccagca tgttccaggt gtcatttgct gacagaggct
     1441 ggcatcaact tgcaagatcc atttctggtt cttttcatcg acaccataaa caacaggagg
     1501 gttttccgaa cagttctggg tttcaatcgt tacgcacatg cacttgtagt ccgggtacgg
     1561 ctctggtttg catgggcaat cggcctgttt tacgaccgtc gcgatgaaat aggtgtttag
     1621 agctgttgct gtggttggtg ttgaggcacc ttgcctgatt gaaccacaga atgtgtagca
     1681 cccgccttcc tttactttaa ggcacagttc ttttgaaagg tatatccgcc actctttgcc
     1741 attggcatct tttccataga caactggcgg ttttgcattg cagtcaacct ttgatactgt
     1801 gacacacatg caccttctgc aatttgggtc accaaacagc aactggacaa tcttggtggc
     1861 ctcgaaccat ttgatgtctt cggggcctgt tgcgccaagg ttttcggcca caaacctcat
     1921 tgggaggact gtccttccac ccactatgaa ggcaaccact tgtgggttgt tcgggtcaat
     1981 ctggacagga tttccattta cctttgctgt tggatttccg atctggattt ccagcgtctg
     2041 gccatcgagt gtttttattg cgacggtttt tgtcgatgcg tcataggcaa tcgttgctcc
     2101 gatttcctcg gccacatacc ttatgagcag gaacatgcgg ttttgtgtaa gaattggtgc
     2161 cgcctccatc gggcctttct gtacatcccc agatttgtag tatttattgc caatctggta
     2221 ttccagcatc agcctgcaat cttcattctc tggcggtatt tcctcaggag gattttcgca
     2281 agttggtgtc ccgcaggcct ctttggattg tgtaaactca tttgcatttg catccactgc
     2341 cctgacaaaa taacaatatt ttgtgccatt tatgatgttg atttcatctt tgaatacatt
     2401 atccgggatt ggaaaatctg tcagcggggt gtcgaattcg gcgccctcgg aggggcccct
     2461 gtaaatccag tagtttattg ctcctgctac tttctcccat ttgagcacgt tgtagccaca
     2521 gcctggtgtt acctccaaac taaacgttgg tgtaagtgaa tatgtgttgc taaaagagaa
     2581 tggagtaaaa atagagcttg tcatggccag aacggtcagc caaataaata gatttctcct
     2641 catatagtgc tcctttcacg tgacgaaata tatgcaacaa aatcagtcgt agctaatatt
     2701 aaattgctct ttatatgcgc tgtcaataag tggatttttt tataatctca attttatttg
     2761 gaccctgttt gtgattttta tctgtttgaa tatgtctgtt gggcattttt cctgtttgga
     2821 aaatcttgta tctctatttt tatggtgttt gagtttttgc ttttttcttc tttaatgcaa
     2881 gttttgatct ggcagaaaat attttcgcct tgtcggtcgt gtttttttgt ttgttgtcaa
     2941 attgtggccc aagttaagga ttttgttaaa aagacaaggt cttgtccgtg tttatttaaa
     3001 ctggcctatc tataataaaa acatgggtgc caaagacagg caaggagggt tcagaatgag
     3061 gagaaatttt gttttttcgc ttttggttgc agttcttgta ttttcaatct tttttgtcaa
     3121 tatgccacat atttttgcgc tgctggggcc tatccatgtt gagcttaata ttatgggaga
     3181 ttcaccatcc tcggtgaggg ctggccaggc gactgctttc aaaatccaca tcaggtttaa
     3241 tgtaaacatc agggtccacg actgggtgaa ggtctggttc ccgattgacg aggcctcgtg
     3301 caagctggag gacatctgtg acgggatgcc gaaaatcact ggcgcaaaag agcacccaag
     3361 gtttgtgcca aatgatgagt acttcaagaa atacaacaac gaagaggaag caaaaattgg
     3421 gaagctttat gaagcgctag atgatagaag gggaaaaacc agctttgaga aatgcgagtg
     3481 tgaggatggt gctccgccgt acagcaactg cgcacctgag ggcaattgca ggataattgc
     3541 cgatccatct ggcctggggt gctggatgac cggtatggtc atgcctgcac tgccaagaga
     3601 tgaaaaaaag cgttatgaat taatgcattc gataatacat gaagcaaggc ttggttacta
     3661 tgatgattgt tattgcagtg gtataccaat aattattaat acatacaaag agcgatcttt
     3721 gcaaatcaat tccccactcg aagtcgaagc atggcggaag ggatacaatc caattgatta
     3781 taatattgga aaaggggctg gcatccttgc cccgatgaca cccggaagat acaggctttc
     3841 tgttgcaaca gcgccggagc caacgcctgt cgaatcggaa tactttgttt tcccgtgttc
     3901 ccaaatttca aggccggtcg tgacgcttgc tggttccgat ccgggcgagc caagcgacat
     3961 tgcaataaaa ttcagtgttg gcgatggtgg cgcactggac aagggaaaat cgaaaatctg
     4021 ggtgcgcttt ccagagagtt ttgtttttcc ggaggcaata aatgccaggc atgtaaaaat
     4081 aaacggggat acccttgagg caagcccaaa gattgacagc cagtcgcacg ccatcgagat
     4141 cacctgtccg gtgaacataa ataatgctgg agaggtcaac atcgcctttt ccccagaggc
     4201 cggaataaga aaccctccca aggagacaga ggcaacactt accgtgtgga cagacccgga
     4261 accggaaaaa gtcgagtcac tgccgtttgt ggtcgagacg aagccaaggg tggaagtgaa
     4321 acccaatatc ttcagcaaca ttgccgaata ttacgcagtg gctttgctcc ccgacgggcc
     4381 aataaaggcc ggaaaggaat tcaaaatcat tttcccggac ggcactttgc tgccagaaac
     4441 tattgcctct tcaagcattg ttcttaatgg aaggaagtat gacggaaaga catcgatagg
     4501 ctggcaaacc gtttcgctca ggtcgtactt cgacattgtg gatgccctga aaatcaggtt
     4561 cctcaaaact gccggcatca ggaaccccct gcccggcgaa tacgagctct catacgaagt
     4621 ggatggcaag gggtatgctt tcccaaaata catcatttca gacaggcatg aagtccttga
     4681 tctcatgcta agcgactacc agggctgtga gcggtccgga tacaaattca cctacatacc
     4741 atcctacaga ggtgatcttg agcctggcga caagctgacg gtggagttcc cgtgggacac
     4801 aagcattccg aaggtgatac caaatgacgc agttctggtt ggcgacaaac cttcaagatc
     4861 ggtgtcggtt gacggcctca agctcaccat aacaacagac acggcaatca aaactgcaga
     4921 tggcggtgca gttgtgtata tcacatggga cgctggtatc ataaacacga gatattccgg
     4981 gaagtacaat ctcaaaatct ggacagagaa ggatgaaccg gtgttttctc cggaatttgc
     5041 catcaccatg ccaaaagtgg aatcatggct tgaattcaag gaccctgcac agccggatgg
     5101 ccttgaatat ggtggctgtc tctggtacaa gacgccgcca atcctctcga taacaagctg
     5161 caatcctctt gcaaagatat ttatgtggta cgacaacaag accgattcaa tcagacaata
     5221 caccggtgaa aagaggctgg actatggctt gttccgtgca acaatctggt attacgcaca
     5281 ggttggagac atcaaggaag aaccgaaatt catgcatctt tgtctggata cggttatgcc
     5341 aaggttttcg atcgccaaac ctgacaagca ggtagttatt gccaataaaa gaacttacgt
     5401 tgtcaaaggc gaacgcgagc catgcgaaat gctcaccgat ggtgacgctg aaaaatacca
     5461 ggcagttgac ggcatttatg tcaacggcaa ggagctcctc gccccggaga tatttgagac
     5521 cgtgagcagg gacgagatta aaatgacctt tgagacaacg attgacctga aagaggggtc
     5581 aaatgttgtc gagataaggg gcattgacca ggcaggaaac gagaagattg agaagagaac
     5641 aattattctg gacaccattc caccaacaat agaaattgtt cggccggacc caaaagaact
     5701 ccagaagccg ggtgaaaaca tcgcaatcca ggtcaagtct gagaccgatg cctcagtgtt
     5761 tatcgacggc cagattgccc agaagataga ggagcttcca gatgggaaaa cagccatctt
     5821 tgaaggtgtc tgggatgtcg tagaaggcga gaacaaagtt aaaatctttt ccaccgatgt
     5881 ggcggggaac actgcagtag cctacctcac attcaagggt ggaacaaagg ctgttaccat
     5941 cgagctctgg attggaaaga ccgacttcgt ggtcaacggg aaaaagatgc caaatctcgc
     6001 cacagcacca acagcctctt cgccaccact tccaaaagca tttgctggca caacattcat
     6061 gccgattgct gatgtcgcaa aagcgcttgg cgcggaggtc aattgggatg caaaaaccaa
     6121 aatgattgca cttactcaaa ccctcgacaa tggcaccaaa aaagtcatcc agctgtggat
     6181 tggcaagaag caggcaaaga ttgacggcaa ggaagtctgg attgatgcaa aaaaggtcct
     6241 ttacccaaca atcatctctg gaaagacgtt gcttccgctc aggtttatcg ccgagaacct
     6301 gaattgtgac gtgcagtatg atgcaaaaac aaaaaagata acactgaccc acccaaaacc
     6361 atgattgttt attggtgaga actagatttt agggttttgc ctggtctcaa gaagctgggc
     6421 ttatttgcca gggtcctcgc cctttttgag tttttcgacc atctgaatga ttttgctttt
     6481 catttttgcc atgtattctg tctcaaactg cattttgcct ttgagtgttt cgtgctgggg
     6541 gtctgcagtt gttgatctca tgatcattgc cttgtcttca atggccttgc cggcagacat
     6601 tctccattcc ttgtaggttt cagagagctg gctgtttttg tactcggtcc agtcccaaat
     6661 cggctttgag agctcagatg gtttttttcc attgtttttt gtgatggagt cgataatttc
     6721 cgatgttttt tgttctatct gcacgaagga atcgtagacc ttttgctcgt tgtcgttcca
     6781 ggtgattttg ccactgccac cgcaagaaac tagcactaag gccgaagtgg ccaccaggat
     6841 gcagcataat gttttcaggt tcatgttttt atttcctcct tgcgttgccg actctgggag
     6901 tcggggaatt ttcaaaaagc atgttttttg tccagacgag tgcgccggat atggcaaaac
     6961 ctccaaggca caacaagatc gggcctgtct ggttgaatat tgcgccaaca accgtttgcc
     7021 ccgtgatgag atttgccagc tcgtggcatg cccatgtcaa cgcccatgag acgagcccaa
     7081 gccctgcgga cacagtcagg gtggtcatga aacttgattt tgttggggtt gcaaacccaa
     7141 agatagataa aatctggttt tcatggagtt ttttgccaaa aatgaaaccc aaaatcaaaa
     7201 agagacccag aaacaccagc cagcctgcaa ggaaagatac ttttgagacc acaacgccaa
     7261 tatttccgcc aaaaactgca aagcacgccg ttgtaaatac atataaaagc gagccttcca
     7321 gtaaagcgtt tattgcccat ccaaatcttg gtttcacgcc cttctattta tccagaatgg
     7381 ttgctctagt caacaaacat ggttgtagaa aaagaggatt ttgttatttg gctggttgtg
     7441 gaaaggccgg gccatttgtt ggggccgacc tgaaattaaa aaaggccggg ttttatctgc
     7501 ccggcctttt agatgttcat cttttttctt ctagtagttg aactcaacgt tcacatttac
     7561 tgtgattgag taattgccgc tgatgatgtt tggtgatact ccacctgctc caccgccacg
     7621 ggaatcttcg cttggtgcca tgctcttgtt tgttatgtat ggctggtatc cagcgatgtt
     7681 gatgtttttg accgaaagga ctttaaggct gtattcgcca agcgcggtgt ctgccttgga
     7741 ttttgcgtcc ctcatagccg tcttgagtgc ttcggcatag aaatctgcct cgtttttcac
     7801 agtgtagttt atggagccaa tctggttgac gccgttttct gttcccttgt cgatgatctc
     7861 gccaatcttt ttgaggtcat aagctgtcac cttgagggtg tagcttgcct ggtagccaac
     7921 aagcctgctc tcgttgtctt ccggaaaata cttgtagact gggttgaggt agtagccaat
     7981 tgtttcgagg tcatcttcct tgcagccgat cttcttgagc gcctcgatga gcgcatttgc
     8041 cttgctgtcg ttctgctgct gggcctgtct tgccgatttg tcagttgttg taatggtcag
     8101 atcgataata aatttgtcag gctttgctgt tactgtccct acaccagtca caaatattgt
     8161 atttttgttc tctgcttcct gaacattctg cgtgtctttt gcacctacaa cgccaaaggc
     8221 cgctatggaa ccgataatta gcgaacctat gactatccct gtcgtcatta ttatagtttt
     8281 cttcattttg ttacctccgt tttttgagtt aggagcgctc accttcagta cactccatgc
     8341 cgtaaaaaag tttttaactg ttcaaaaaaa tgtcaaaaat actgatttta tttgacctgg
     8401 aacgagtgtt agtcaatgcc gggagtacca ctattaatta taaaaagaaa atgtttttgg
     8461 aacaatcaga aagaacgtga tctattttgt tttttccctc aaatagataa accagtaaga
     8521 aataccaaca aataacgccc ctccaacaat attgccgagc gtgacccaca agagattgtt
     8581 tgccatggcg cctggcgaga ggtgtgaaaa atcctgggca gttttgccaa ttgatgacca
     8641 aaatgattgc cctgcaaaat tttttatgag tatggcgatg gtgccaaaat acatgttggc
     8701 gacactgtgt tcaaatcctg cggccacaaa agcggtgatt ggaaagatta ttgccattat
     8761 cttgcatgtt gttgttttgg cactgtatgt gagccataca gccaggcaaa cgagcacatt
     8821 acataaaatt cctagaaaaa acatctgtga tggctgaagt gcaaatttgc cctctgcaat
     8881 cttgagcgcc caaagtccca cctggccgcc agagaagaga tattgtccgc cgaggaagat
     8941 tgttgcagct attatcacgg aacctacaaa attgccaaaa tagactatcg cccagttgcg
     9001 aagaagtttt gagaaggtga tttttttgtt tgcccaggcc atcgctatca agttgttgcc
     9061 ggtgaaaagc tctgcccctc caacaacgac cagtatgagt ccagtgcaaa atgcaaatcc
     9121 tgcgagcatt tttgatatcc cgtaagggat tgcctctgag cccacaagaa cggtggacga
     9181 aaagaagcca cctattgcaa tgtatgcacc cgccagcaac gaaagggtga atgtttttgt
     9241 aaagctctgg ttggcttttt taacgcctat ttcttcagct ttttttgcca tttcatgagg
     9301 tagcagcgaa tcgaatgatt gattgtccat ttttcaccag ccttttggga tgtaatttat
     9361 tgccccgatt aggatttttt caaggttcat atttttgaaa aaaatagagt attgacttcc
     9421 cattaaatgg tgctaaaaac acaggaaaac atttttttgg ttatttttaa aatgaaagat
     9481 agaaaatacc aatttttaga gtcagtaaat aatatttacc ggagaaccaa aaagccggtc
     9541 cattactcta gtgttgcgga ttttttggga gtgtcactct caaccgcata caacatcctt
     9601 cttgaattgt catccagggg tctcctcaaa aagatttatg aacctgatgg aaagaagggc
     9661 agggcgaaga ttttgttcat gcccacagag gatggcacgc ttactgcaag ccgtgaaaat
     9721 ggtgggaacc ttatggattg catcctggct tgccactttg ccattatgac tgcagtgatg
     9781 aaggccccgg agattctctc ctcaacaaga tgttgcaagg attcttccaa atcggttgtc
     9841 gttgggctca tgaccggaat atgggataaa ataattgaaa acatatcatg tgatcaaacc
     9901 tccatggagt tcctgaacag ggtttcaggg accaagaggc agtttctcaa atctatttca
     9961 aaactctcag atgttgaaat taggtcgtta tgtagggcgc tgacatcatt tttcttcatg
    10021 gcaaataatc taatcattat gggaagggga tgagagaaat tatgaatgcc attgaaaatg
    10081 caaaaagaat cgctggaaat gttgttgtaa atgctgccat gaattacatc accaatggta
    10141 atgttgaaag gaagattgat gaccttttta acattgcgct taaacttgcc tggagaccat
    10201 gcgacaggga acccattctc cagctcaaaa gcagattcag ggaccctgat tgcccacaga
    10261 aaaagcttct agtaaacgtc ctcaaatccg atccgaactg gttgaaaaaa gccgcggcca
    10321 atttttttgt aaattcaatc attgttggca gaaaaaaaac gctggagctg gaaaaaagaa
    10381 ataaaatgat gctcccgttc tttctcctta ttgatccaac agagagatgc aattacaggt
    10441 gcaagggctg ctgggccggg aattttgacc catgcgacat gccgtatgag gtttttgatc
    10501 gcctgctttg cgaggccaag gagcttggga tttattttgt tacgatcagc ggaggagagc
    10561 cttttcttta caaggatctt ttcaaggtca tggagaagca tcctgacatg acgtttcatt
    10621 tctacacaaa tggttcattg cttgctgatg aggaatttgc tgataaattc gttgcgcttg
    10681 gaaatggact tccatgtttt tcacttgaag gttttgaaga aagaactgat gaaagaaggg
    10741 gcaagggtgc ttttgtcaag gtcatgaagg ccatggacaa cctgagggag aggagatatc
    10801 cttttggaat ctcctgcact gcaacttcgc agaactacaa agaaataaca tctgacgagt
    10861 tcgttgatgc catggtcaaa aaaggggtcg tgcttggctg gtattttatg tacatgccaa
    10921 taggcaggga cccggatttt gaaatcatgc tcaagccaga acagcgcaaa tacatgtggc
    10981 agaggacaac aaaaatcagg aatgagaaac cgatcgtgtt tgcagatttc tggaacgacg
    11041 gcccagtcac cgatggctgc ctggctggag gaagaaggta tgtacatata acggccgatt
    11101 gccatgttga gccttgtgct tttgtgcatt tcaacagacc tgaagatagt gtaagagaga
    11161 aatcattgtt gaaagtgctg gaagagtcga agttgttcaa tgcgatgagg gcaaggcaga
    11221 atcctgccta tgaatccaat cccatgaggc catgctggat tgtggacaga ccgtgggccc
    11281 tgagagatgt tgtaaaagag gtctctgccg atgcgtctga agctggctcc atccatctca
    11341 tggatgaaaa agtcgctggt gaacttgaca ggaga
//
LOCUS       DAOSRI010000018        11291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5671_length_11291_cov_4.016376, whole genome shotgun sequence.
ACCESSION   DAOSRI010000018 DAOSRI010000000
VERSION     DAOSRI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5671_length_11291_cov_4.016376"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..4432
                     /locus_tag="PK717_01040"
     CDS             266..4432
                     /locus_tag="PK717_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46292.1"
                     /translation="MRKNLSFFLAVLLCSASIAFPQYSFSKNGVPPYLIQLSATASKG
                     QIALSWPQQQASTIYVYRDFGGPNSKPVATLLPSETTFIDKPIPGPHIYGLLVVGKDG
                     RIAHRTAMLPVYLPKDCEDPSQMIIKLWINRFDMVVDCQSTTIKTAPEIINGSTFVSI
                     RPIIEAAGGSLSWDGTTKTATVYLQNHFVMLTIGKNIAKVDGVEKPILASNPAIVPLI
                     KNSNTMLPFRFIVESLGGRVNWDGTEKRIEIFFALSELAKIKRYLEPQSLVAMGEIGP
                     VVKVSAVEEIKTNDPLETFMSGAKILVASGKDYNVVSISDALDPMGRKLSKTNFPLWM
                     KQTGNVRAYKVSYDPLTMKIKPFTSVILVDLGNGDIFDPTLFPFLLEFGPVDFEGQQQ
                     YKNKFDAKIAKFSTSWPVGNIFNDSSRIAFLDFDVVISGKISVSNPNCTPCARTMGEA
                     EFAIKSICDEIQANSLRIFVPMIASLFDVSMATSQDNGFADLSLKGGCGFFPTSTCPD
                     MKDQFAEMEFNFSDMRPYQATDDPQKSGQIVKGIFKLNPSNASEQGYESKDSNPKSQG
                     LFAVKIDDSPKPKHMRIERKSNPKKFGEFLTPSLPPSLQPIPGCTLSMKVPILNTPSG
                     RQSGIFEVAFSPAVSVTYPFERTVGFFGVEQEKKTLVLCNGQFVAQAGFECPCGELRG
                     IEDASLKVDKTKPGQPKVVVDVRINPEVAFNPPESDVYVALFRNDVEISKQNVDKSNP
                     QVQFVDEKIGAGEVYAYCIKLYQNGMEIDKWCHDETIVPNPWTISAAFEDGKQSKSET
                     ILPGRATQAKIKIGNTDAQEIQVQVRVDNICPKWTVKIDGKNDKWVTVGPDREEFVSV
                     VVEPDETITPGDSCEVGIVIQSGLQKITLKYKITIESPTCSFKCEWEEGGAGITNDVS
                     PGIAKYQMFTIKNTGSEQNKFVVDFTTDQDASTMGGVLWTVNFKDIMQGQSITIDAGK
                     SVNIYMIVKASMSTQDGKSIKIDVSVKGCGETYKLEWVVTCKLQECEFDVGWTKGTST
                     RKYTTNPGDSWYESFTIINYGTQDMLFNVKVENEGEKLVTLLDSYEFSIAGGDTHKAK
                     MQIKTPDSARIGEARLKITVTCGKTEKTLKLTVNIVKGESCSYTASWIITGNGTLNMD
                     MPVDEVVTASIRIKNKSKKAELFAILTDRSDSSWESGFDTAKGLKQTFTLDPGEETQK
                     LILWAKAGKNTKPGDACSVKVSVKACSTSLELMWNLVCVPHADLDVSVSNKVQSAKWT
                     KDGSLAMEGVMAFTRQGAGSIKGISYQFEWIDVDGGGVTLGKTSQYSLDLEIYTGVPA
                     FSYEHRVPKSIIDKLATSGSKRIKIIVSLNFLFKEETVVTKKAEFIVDIPPKTSLIQS
                     DSKGLVPIAV"
     gene            complement(4504..5706)
                     /gene="rocD"
                     /locus_tag="PK717_01045"
     CDS             complement(4504..5706)
                     /gene="rocD"
                     /locus_tag="PK717_01045"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOR46293.1"
                     /translation="MNSEYFMNLERQYGANNYESLEVVLSKGEGVWVWNVEGIQFMDM
                     LSAYSAINHGHRHPKITQALKDQIDRLTLTSRAFFNDKLPLFTEKICKFTGKEMFLPM
                     NTGAEAVETAIKCMRKWGYEVKGIEPETGEIIVFAENFHGRTTTIISFSTDPGSHDHF
                     GPFTPGFKIVPYNDAEAVEKAITDKTIGILIEPIQGEAGVNVPDEGYLTNLQKMCRKH
                     KILFVCDEIQTGFGRTGKRFCFDHEGLDPDIIIMGKALGGGVYPVSGVAANKEILSLI
                     KKGQHGSTFGGNPLACVAGITAIEVLQEEKLIENSATQGAYFMKLLKQLEGNSKVEKV
                     RGKGLLIGVVIKQSAGPAKDYVKKLRNEGLICKETHDKIIRFAPPLVITKDQIDWAFS
                     KIQKILGD"
     gene            complement(5811..5886)
                     /locus_tag="PK717_01050"
     tRNA            complement(5811..5886)
                     /locus_tag="PK717_01050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5851..5853),aa:Thr,seq:cgt)
     gene            complement(5965..7029)
                     /locus_tag="PK717_01055"
     CDS             complement(5965..7029)
                     /locus_tag="PK717_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46294.1"
                     /translation="MGDTNKPYDWEVVEYDLKNASFELFDKYYTIYNRIYKEERPDEP
                     ALPPDILKQRIQGNDPSWCNRYFAVFKDGAYIGTGSMQFVKPGVEADEFKFLSTFYIR
                     TLPECRRKGVGTAILKKMTQEALKMDIKIIEMYSMLDSGKVFCEHFQGKVVSNFGFNK
                     LDIKDIDWKMVESWKTEAETKTPDIKIEFHDKLTGKDWDQYLEMEVTFFKELSLFDEE
                     PLFDEEFFRKDFTEYEKKRIEEGTRDAIIAVAREANGTIVGYTEISYDKREPYKIDQD
                     MTGVLKEKRGKNLGKWLKAEMLLKIRETKPDVIFIATGNDTKNEHMLKINRKLGFRQS
                     FVSLEYSFKIHDLATKLGLE"
     gene            complement(7117..7998)
                     /locus_tag="PK717_01060"
     CDS             complement(7117..7998)
                     /locus_tag="PK717_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46295.1"
                     /translation="MNGKIEFFLPGMFTVDGETGIYPAVSITGKTCILMCNHCMAHIL
                     CTMPDCSTPEKLVEFALKANSSGARGILVSGGSDKNGKLPWETFWPALLEIKQKTKLI
                     LSVHSGFADEDQARLLKQAKIDQALIDVIGSDDVARRIYKLPNGIAARAVDAVFQSGL
                     HVVPHVLVGLDYGKPSDEEKALEMIAGYSPKTVVIIALRPSRHTPMGEANPPTPERVA
                     EIIGLAKSLMPRTFINLGCARPVGEHKRQTDILAIRAGVDGIAVPSKQAEEEAARLGL
                     TIIKHPTCCSLISLGSM"
     gene            complement(7995..9635)
                     /locus_tag="PK717_01065"
     CDS             complement(7995..9635)
                     /locus_tag="PK717_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOR46296.1"
                     /translation="MNDYVKMTFELLGGLALFIWGMQMMGDGLQKAAGDKLRYILEKL
                     TRKPIYGVLVGAIITGIIQSSSATTVMLVGFVNAGLMSLSQALGVIFGANIGTTVTAQ
                     LVAFKIEWIIMPAIFLGVVLRLFGTKKALRLFGEVLLGFGILFLGMSFLSQSVLFIKD
                     VESVKSFLASVSGNLFLGILAGAIFTGIIQSSSATSGLVIALIGAGLINLTGAIGLMM
                     GANIGTCVTALLASIGGNLGARRVALAHLMFNVIGVVVFIPFVIPVTSLMQALSSEPA
                     RQTANFHTFFNISMTIIMLPLTSYYEKLIKWIMPGKEMVAEHGIKFISDKILVTPSLA
                     LEQATQEMARMLSIAEGMIKSTREMIKTRNHRLITLIMDNENTVDSLQMAITTFISKL
                     TQKSLSDEQADRAIVLLKAVHDVERIGDHATNLAELAEGMIDNNVRFSDAGLASLFEL
                     YDTVEESTDLVKRALTDYDKKLARQMFEKEKAIDKKTDQIRDEHIERLKTNDCKPDNG
                     IFYLDMSSNLERIGDHCVNIAQSVLGDVVHADEGHAAE"
     gene            complement(9694..11034)
                     /locus_tag="PK717_01070"
     CDS             complement(9694..11034)
                     /locus_tag="PK717_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR46297.1"
                     /translation="MDEYRDTIINKPISTALWSLALPMMLESVVLTILNLTDMFWVGR
                     LGKEALAAAGIVGMLMITISSIAFGLSIGVIAIVSRRIGERNGKGAAEGWLNGTIIGL
                     AIGILVTLIMIVLQNPILDLFGMSPETLELGKQYYIWIFAARPLLFISSINGGALRGA
                     GRPKLTLIANIIAVVLNMFLDPVLIFGWLGFPELGIAGAAIATIFSEFVAASILTWFS
                     YKTFSEMSEGLIRIKSHLMMNVLKIGLPSSLQTFVMFTARTFLMGIVSRFGDAVTAAY
                     TVSIRAQEFITLPVVGMGNAVATLVGQNLGARKPERSEQAVYKALFYNTIIVVSLSIL
                     FVAFAKTIVGLFNNDPTVIDEGRIILSTTFIANILMPLSFIPQRGLSGAGDTITALMI
                     SAGTQWVLQIPLCFVLANMFGQYGVWWGINAGSIVMAIISISVFRTGRWKRHKV"
BASE COUNT         3141 a         2421 c         2387 g         3342 t
ORIGIN      
        1 catctcaatg aattatttta aagattatta cttcaatttt taaacaaaag tatgaatttc
       61 ctgtttttgt gatggatttg tgaaattttg atgtcgtaga aacaaacaca agaatgatgt
      121 tttgctcgat ctgctaaagc ttttttatca tttgtggaaa tgttaagaag gtcacatcat
      181 ttcagaaaaa aatcatttta tcacaatgga aaatcgcttg attcgtgaag aaatgcgatt
      241 atttttctag caaaaaggag gagaaatgag gaaaaatctt tcattctttt tggcagttct
      301 tctttgttct gcttcaattg ccttcccgca atattcgttc tcgaaaaatg gtgtaccacc
      361 ctatcttatc cagctatcag caacggcatc aaaaggccag atagccctct cttggccgca
      421 gcagcaggcc tccaccatct acgtatacag ggattttggc ggccccaact ccaagccagt
      481 ggcaacactt cttccgtctg aaaccacctt tattgataaa ccaatcccgg gccctcatat
      541 ttacgggctt cttgttgttg ggaaagatgg aaggattgcc cacagaacgg ctatgctccc
      601 agtttatctt ccaaaagact gcgaagatcc ttcacaaatg atcatcaaac tctggataaa
      661 cagatttgac atggttgtcg attgccagtc aacgacaatc aagactgcac cagaaattat
      721 taatggttct acatttgttt cgatcagacc aatcatagaa gctgctggtg gttcactttc
      781 atgggatgga acaaccaaaa ctgccaccgt atatttgcag aaccattttg taatgctcac
      841 cattggcaag aatatagcta aagttgatgg agttgaaaaa ccaattctgg catccaatcc
      901 tgcaattgtg cctttgataa aaaattcaaa cacgatgctt cctttcagat tcattgttga
      961 atcacttggt ggcagggtca actgggacgg cactgagaaa aggattgaaa tattttttgc
     1021 cctttctgaa ctagcaaaaa taaaaagata ccttgagcca cagtcgcttg ttgcaatggg
     1081 cgagattggc ccagtggtaa aagtttctgc agtcgaagaa atcaagacga acgatccttt
     1141 ggaaactttt atgtctggtg cgaaaatact tgtggcatct ggcaaagatt acaatgttgt
     1201 ttcaatatcg gacgctttgg atccaatggg acgcaagctt tcaaaaacca acttccccct
     1261 ctggatgaaa cagacaggca atgtcagagc ctacaaggtc tcgtatgatc cgttgacgat
     1321 gaagataaaa ccgtttacat cagtaattct tgtcgattta ggaaatggtg atatctttga
     1381 tccgacccta ttcccatttc ttttggaatt tggcccagtc gactttgagg gccagcaaca
     1441 gtacaaaaac aagtttgatg ccaaaattgc aaagttctcg accagctggc ctgttggcaa
     1501 tattttcaat gactcgtcta gaattgcttt cctcgatttt gacgttgtaa tttcaggaaa
     1561 aatatcggtt tccaacccca attgcacccc atgtgccaga acgatgggtg aggcggaatt
     1621 tgctatcaaa tcgatatgtg atgaaatcca ggcgaactca ttgaggattt ttgtgccaat
     1681 gatagcatct ttgtttgatg tttcgatggc aacctcccag gacaatggtt ttgcagatct
     1741 ctcgcttaaa ggtggatgcg ggttcttccc aacctcaaca tgtcccgaca tgaaggacca
     1801 gtttgcagag atggaattca atttctctga tatgagaccc tatcaggcaa ctgatgatcc
     1861 gcagaaatcc ggtcagattg taaagggtat tttcaagctc aacccgtcaa acgcttccga
     1921 gcagggatat gaatcaaagg attccaatcc taagtctcag gggctttttg cagtaaaaat
     1981 tgacgactcg ccaaaaccaa agcatatgcg gattgaaaga aagtcaaatc caaaaaagtt
     2041 tggagaattt ctgactcctt cccttccgcc atctctccag ccgataccgg gttgcacact
     2101 ttcaatgaag gtgccaattc tgaatacccc ttcaggaagg cagtcgggca tttttgaagt
     2161 tgccttctca ccagctgttt ccgttaccta tccttttgag cgcacggttg gattttttgg
     2221 tgtagagcag gagaaaaaaa cactggttct ttgtaatgga caatttgttg cccaggctgg
     2281 ttttgagtgt ccatgtggtg aattaagagg aatcgaggac gcatcattaa aagtcgacaa
     2341 gacaaaacct ggccaaccga aagttgtcgt ggatgtcagg ataaacccag aagtagcatt
     2401 taatcctcca gaatcagatg tatatgttgc cttgttcaga aatgatgttg aaatctcaaa
     2461 acaaaacgtc gataaatcga accctcaagt ccaatttgtt gatgaaaaaa ttggtgccgg
     2521 ggaagtttat gcctattgca tcaagcttta ccagaatggt atggagatag acaaatggtg
     2581 ccacgatgaa accattgttc caaatccatg gacaatctcg gctgcatttg aagatggaaa
     2641 acagtcaaaa tcagaaacga tactcccagg acgggcaaca caggcaaaga taaaaattgg
     2701 gaatactgat gctcaggaaa ttcaagtgca ggtaagggtt gataatatct gccccaaatg
     2761 gactgtcaaa atagatggaa agaatgacaa gtgggtgaca gtgggacctg atagagaaga
     2821 gtttgtttcg gttgttgttg agccggacga aacaataaca cctggcgatt cttgtgaagt
     2881 tggtatagtt attcaatctg gtcttcagaa aattactctc aaatataaaa tcaccattga
     2941 gtcaccaaca tgctccttca agtgtgaatg ggaagagggt ggagctggaa ttacaaatga
     3001 tgtttcacca gggattgcaa aatatcagat gtttacgata aaaaatactg gttctgagca
     3061 aaacaaattt gtggttgatt ttacaactga ccaggatgcg tccacaatgg gcggagtgct
     3121 atggacagtg aatttcaagg atattatgca aggccagtcg ataactattg atgctggtaa
     3181 atctgtgaac atttatatga tagtgaaggc atcaatgagt acgcaagatg gaaaatccat
     3241 caaaattgat gtatcagtaa agggatgtgg agagacatac aagcttgaat gggtagttac
     3301 atgcaaactc caagagtgcg aatttgatgt gggttggaca aaaggtacat caacaagaaa
     3361 atacacaaca aaccccggtg attcttggta cgagtcgttc actattataa attatggaac
     3421 acaggacatg ttgtttaatg tgaaagtgga aaatgaaggt gaaaaacttg taactttgtt
     3481 ggacagctat gaattctcta ttgctggtgg tgatacacat aaggcaaaaa tgcagatcaa
     3541 aacacccgat tctgccagaa ttggtgaggc aagattaaaa ataactgtaa cgtgcggcaa
     3601 gacagagaaa acacttaaac taacagtcaa catagttaaa ggagaatctt gctcttatac
     3661 tgcttcatgg atcattactg gtaatggtac cctcaatatg gatatgcctg tagatgaagt
     3721 tgttactgca tcaatcagaa tcaaaaataa gagtaaaaaa gctgaattat ttgcgatact
     3781 cacagataga tctgattcca gctgggaatc aggttttgac acagcaaaag gcctaaaaca
     3841 aacgtttaca cttgatcctg gtgaagagac ccaaaagttg attctttggg ccaaagccgg
     3901 gaaaaacaca aaaccaggtg atgcttgttc tgtgaaagtc tcggtgaaag catgctcaac
     3961 atcccttgaa ctcatgtgga accttgtttg tgtaccgcat gctgatctag atgtttctgt
     4021 ctcaaacaaa gtacaatccg caaaatggac aaaagatgga tcgcttgcca tggaaggtgt
     4081 gatggctttt acaagacagg gtgctggatc aattaaaggg atctcctatc agtttgaatg
     4141 gattgatgtt gatggtggtg gtgtcacact tggcaagaca tcccaataca gccttgatct
     4201 tgaaatctac actggcgttc cagcttttag ctatgaacac agagttccga agagtatcat
     4261 tgataaactt gcaacatctg gaagcaaaag gatcaaaata attgtctctc tcaatttcct
     4321 tttcaaggaa gaaaccgtgg ttacgaaaaa ggcagaattt attgtggata ttcccccaaa
     4381 aacttctctt atacagagtg attcaaaggg attagttcca atcgctgtct gatagataaa
     4441 aacaaataaa aaaagccggt cattgaccgg ctttttttat ttgttggttg atttgttctt
     4501 ttatcagtcg cctaagattt tttgtatttt ggagaatgcc cagtcaattt gatcctttgt
     4561 aatgacaagt ggtggtgcaa aacggattat cttatcgtgg gtttccttac agattaagcc
     4621 ttcattgcga agttttttga catagtcttt tgccggacct gcagactgtt tgattacaac
     4681 acctatcagc aagcctttac ctcttacttt ttcaaccttg ctgttgccct caagctgttt
     4741 caggagtttc atgaaataag caccctgggt tgctgaattt tcaatcagtt tttcttcctg
     4801 gagtacttcg attgcggtta ttcctgcaac acaggcaagg gggtttccac caaaagttga
     4861 accatgctgg ccttttttga tgagactgag gatttctttg tttgctgcca cacctgagac
     4921 gggatataca ccgccaccca aggctttacc catgatgatg atgtccggat caagtccttc
     4981 gtggtcgaag cagaatctct tgcctgttct gccaaagcct gtctggattt catcacatac
     5041 aaaaaggatc ttgtgcttcc tgcacatctt ttgaagattg gtcagatatc cttcatctgg
     5101 aacattaacg ccagcctcac cttggattgg ctcaataaga atgccaatag ttttgtccgt
     5161 gatggctttt tcaactgctt cagcatcgtt atatggaacg attttgaagc ctggggtaaa
     5221 tggaccaaaa tggtcatggc ttcccgggtc agttgaaaaa ctgatgatcg tcgttgttct
     5281 gccgtggaaa ttctctgcaa aaacaatgat ttcaccagtt tccggctcaa tgccctttac
     5341 ctcatagccc cacttgcgca tgcatttgat ggcagtttcg acagcttctg ccccagtatt
     5401 catcggaagg aacatttcct tgcctgtgaa cttgcagatc ttctccgtga aaagcgggag
     5461 cttgtcattg aagaatgccc tggatgtaag cgtcaatctg tcaatttgat cttttagggc
     5521 ctgcgtaatc tttgggtgtc tatggccgtg gtttatggct gaatacgcac tgagcatgtc
     5581 catgaactgg atgccctcga cgttccagac ccaaacccct tcgccttttg acaacactac
     5641 ctcgagcgac tcatagttgt ttgcgccata ctgtctctca aggttcataa aatattcgga
     5701 attcatgaac acctcctgga tttgtgagaa acaaataaga ttgtaccagc ctaaaattat
     5761 aggtcaacaa ggcttgtggt tatagaatta ttgtgatatg gggatattga tggagccgaa
     5821 gaggggattc ggacccctga cctgctgatt acgaatcagc tgctctacca gctgagctac
     5881 ttcggcacgg tcagatatta taacaaacac cataatccag tccagtgcaa ttggtaaaac
     5941 tcctgaagct ggtggctgct gtttctattc aagccccagt tttgttgcca gatcatggat
     6001 tttgaaagaa tattctaaac ttacgaatga ttgtctaaaa cccaattttc tatttatttt
     6061 aagcatgtgc tcattctttg tatcatttcc agttgcaatg aaaataacat caggttttgt
     6121 ctctcttatc ttgaggagca tctctgcttt taaccatttg ccaaggtttt tgcccctctt
     6181 ctcttttagg acgccggtca tgtcctggtc tatcttgtat ggctctcttt tatcgtatga
     6241 aatttcagtg tatccgacaa ttgttccatt ggcttccctt gcaactgcta tgatagcatc
     6301 tctagttcct tcctcaatgc gttttttctc atattctgtg aagtctttcc tgaaaaactc
     6361 ttcatcaaag agtggttcct cgtcaaaaag ggagagttct ttgaaaaagg taacttccat
     6421 ctccaaatat tgatcccaat cttttccagt gagtttatcg tgaaattcta tttttatatc
     6481 aggcgttttt gtttcagctt cagttttcca gctctccacc atcttccagt caatatcttt
     6541 gatatccagt ttgttgaagc cgaagttaga aaccacctta ccctgaaagt gctcgcagaa
     6601 aacttttcct gaatcaagca tgctgtacat ttcaattatc ttgatatcca tctttagcgc
     6661 ttcttgggtc atctttttta gaattgcagt accaacacct ttacgcctac actctggtaa
     6721 agtccttatg tagaatgtcg acaaaaattt gaattcatca gcttccacac ctggctttac
     6781 aaattgcata cttcctgtcc ctatgtatgc accatccttg aaaactgcaa aatacctgtt
     6841 gcaccaagac gggtcattcc cctggatccg ttgttttaaa atatctggtg gtaatgctgg
     6901 ttcatccggg cgttcttctt tatatatccg gttgtatatg gtataatatt tatcgaagag
     6961 ctcaaaagag gcgttcttca gatcgtattc gacaacttcc caatcatatg gtttatttgt
     7021 gtctcccatg gcattgcatt ttatgtgata ttttatgaat ggcaataatt gaggttttgt
     7081 tcccaataag tatatgtatc aagaatctga tccggtttac attgaaccca acgaaataag
     7141 gctacaacat gtcgggtgct tgattattgt caaacccagc cttgcggcct cttcttctgc
     7201 ctgtttgctt ggaactgcaa tcccatcgac cccggccctt attgccaaga tgtcggtttg
     7261 ccttttgtgc tccccaaccg gcctcgcgca acccagattg atgaatgttc ttggcattaa
     7321 tgattttgcc aggcctatga tctcggccac tctctctggt gttggcggat ttgcctcacc
     7381 catcggagtg tgcctgcttg gcctgagagc tattataacc actgtttttg gtgagtatcc
     7441 ggcaatcatc tccagcgctt tttcctcgtc agaaggcttg ccgtaatcaa gcccaacgag
     7501 gacatgtggc acaacatgga gaccagattg gaaaactgca tccactgccc ttgcagcaat
     7561 cccgtttggc aatttgtaaa ttctcctggc tacatcatca ctgcctatga catcaatcag
     7621 tgcctggtca atttttgcct gtttaagtaa tcttgcctga tcttcatccg caaaaccgga
     7681 gtgtactgac agaatgagtt ttgttttttg ttttatctcc agaagtgctg gccaaaaagt
     7741 ttcccagggc aatttgccat ttttgtctga tccacctgaa acgaggattc ctcttgctcc
     7801 tgaagagttt gctttcagcg caaactcaac aagtttttct ggcgtcgagc aatctggcat
     7861 ggtgcacagg atgtgcgcca tgcaatgatt gcacatcaag atgcatgttt tgcctgttat
     7921 tgataccgct ggataaatgc ctgtttcacc atcgacagta aacatccccg gtagaaaaaa
     7981 ctctattttg ccgttcactc tgcagcgtga ccctcgtcag catggacaac atcaccgagt
     8041 actgattgcg caatattgac gcaatggtcg cctattcttt caagattgct tgacatatcc
     8101 agatagaata ttccgttatc aggtttgcag tcatttgttt tgagacgttc tatatgttca
     8161 tcccttatct ggtcggtttt tttgtcgatt gctttttctt tttcaaacat ttgtcttgcg
     8221 agctttttgt cgtagtccgt gagcgctcgt tttacaagat cagttgattc ttcgacggta
     8281 tcatagagct cgaatagtga agcaagtcct gcatccgaga acctgacgtt gttgtctatc
     8341 attccttcgg caagttctgc caggtttgtt gcatgatcac caatcctctc tacatcatgc
     8401 acagctttca gcaagactat ggcacgatct gcctgttcgt cagagagtga tttttgtgtc
     8461 agcttggaaa taaaagttgt gattgccatc tgcagggaat caactgtatt ttcgttgtcc
     8521 atgatgagtg taataagcct gtggttcctt gttttgatca tctctcttgt tgattttatc
     8581 atgccctctg caatgctgag catccttgcc atctcttgtg ttgcctgttc aagggccagc
     8641 gatggagtta caaggatttt gtctgagatg aattttattc catgttcagc gaccatctct
     8701 ttgcctggca ttatccattt gatcaatttc tcatagtagg aagtgagggg gagcatgatt
     8761 atcgtcattg atatattaaa gaaagtatgg aagttggcag tctgccttgc tggttctgat
     8821 gataatgcct gcatgagcga ggtgactggg ataacaaaag gtatgaaaac cacgactcct
     8881 ataacgttga acatcagatg cgccaaagca acccttcttg cgccaagatt gccacctatt
     8941 gaagcaagca gtgccgtaac acatgttccg atatttgcac ccatcatcag gcctattgca
     9001 cctgtcaggt ttatcaggcc tgcgccgata agagctatta caagacccga tgttgcagat
     9061 gaagactgga ttattccggt aaaaattgct ccggccagaa tgccaaggaa gaggtttcca
     9121 gagacacttg caaggaaaga tttcaccgat tcaacatctt ttatgaaaag gactgattgt
     9181 gacagaaaac tcatgcccag gaagaggatt ccaaaaccca ataaaacttc tccgaagagt
     9241 ctgagcgcct tttttgttcc aaacagtctt aatacaaccc caagaaagat tgcaggcatt
     9301 atgatccatt caatcttgaa tgccacaagc tgtgcagtta ctgttgtccc gatattagct
     9361 ccaaaaatta ctccaagagc ctgggaaagt gacatcaatc cagcatttac aaaaccaacc
     9421 agcattacag ttgttgccga agatgactgg attataccgg taatgattgc cccgacgagg
     9481 acgccgtaaa ttggttttct ggtcagcttt tcaagaatgt atctgagttt atctccagcg
     9541 gctttctgga gaccgtcacc catcatctgc atgccccaga tgaaaagggc aaggccacca
     9601 aggagttcaa atgtcatttt cacataatcg ttcatgtttt acctcgcgag gagtgattgt
     9661 aagagttgtg ttagaaattt gcaaacaagc tggttaaacc ttgtgtcgct tccagcgtcc
     9721 agtcctgaat acagaaattg agattatggc catcacaata gagcctgcat tgatacccca
     9781 ccagacgcca tattgcccaa acatgttcgc aagaacgaag cacaaaggga tctggaggac
     9841 ccattgtgtg cccgcagaaa tcatgagagc agttatcgtg tccccagcgc cactcagacc
     9901 cctttgtgga atgaagctga gtggcatgag aatgtttgcg atgaatgttg ttgagagtat
     9961 tattctgcct tcatcaatta ccgttggatc gttattaaac aacccaacta tggtttttgc
    10021 aaatgccaca aaaagtatgc tcagagaaac aactatgatt gtattgtaga agagagcctt
    10081 ataaacagcc tgctcggacc tctccggttt tctggccccg aggttctgac caacgagtgt
    10141 tgcgactgca ttgcccattc ccactaccgg caacgttatg aattcctggg cccttatcga
    10201 aaccgtgtat gctgccgtga cagcgtcgcc aaaccttgag acaatcccca tgagaaatgt
    10261 ccttgcggtg aacattacaa atgtctggag tgacgaggga aggccaattt tcaggacatt
    10321 catcatgaga tgggatttta ttcttatcag accttcagac atctctgaga atgttttgta
    10381 ggagaaccat gtaagtatgg atgcggctac aaattcggag aaaatggtcg ctattgcagc
    10441 tcctgcaatg cccaattctg gaaatccaag ccagccgaag atgagtaccg gatccaggaa
    10501 catgtttaat actactgcaa tgatgtttgc tataagtgtc agttttgggc gtcctgcacc
    10561 ccttaatgct cctccattaa tggaggagat gaaaaggagt ggtctggcag caaatatcca
    10621 gatgtaatac tgttttccaa gctccagtgt ttcaggtgac attccgaaaa gatccaggat
    10681 cggattttgt agaactatca ttatcagggt aaccagaata cctattgcca gccctatgat
    10741 cgtaccgttg agccaccctt ctgcggcacc ctttccgttg cgttccccga ttctcctgga
    10801 gacaattgct atgactccaa ttgagaggcc gaatgcaatt gaggaaattg ttatcatcag
    10861 cattccaacg atgcctgcag cagcgagtgc ttccttgcca agtctcccaa cccagaacat
    10921 gtcggtaagg ttcagtatcg tcaacaccac tgattcgagc atcataggca aggcaagtga
    10981 ccagagtgct gtggaaatgg gtttgtttat gattgtgtct ctgtattcat ccataggccc
    11041 tagattctat cccgaaataa aatttgaatc tttaaaacaa aacccaagcc atgatgttgt
    11101 cgaaaaaatc gctatttata gaggtttgcg tttcattctg gtatggtggc cataaaaaaa
    11161 ccggcctcaa aatgaggccg gtttcaatat ttggtttttt aatggattac tgcttatcct
    11221 tgagttcttc caggttcttg ccagcctctt ttgcaaaatt cacaaactct tttagtgttt
    11281 cacacatgta a
//
LOCUS       DAOSRI010000019        11235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5704_length_11235_cov_3.842665, whole genome shotgun sequence.
ACCESSION   DAOSRI010000019 DAOSRI010000000
VERSION     DAOSRI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5704_length_11235_cov_3.842665"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..379)
                     /locus_tag="PK717_01075"
     CDS             complement(68..379)
                     /locus_tag="PK717_01075"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOR46298.1"
                     /translation="MVQKSRVLKRKTKTSTKPALQVAHNASVKHTQYHLVPDKEKKWI
                     VKKVDTEKVMMHASTKDDLLKKASDYIKNHGGGSLRVHKLDGTIQEERSYGFNSRSSK
                     G"
     gene            693..1121
                     /locus_tag="PK717_01080"
     CDS             693..1121
                     /locus_tag="PK717_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HOR46299.1"
                     /translation="MKLIDPNDVPDTNKIEKFLGKTFVFWKQISDLIDQKYPGIFTPE
                     WLYAGKKHGWSLRYKKNKSFCTMIPGKDRLAIQIVFGKDERSKVEEIKENLSSRTKNA
                     YESATTYHDGKWLLLNVDGEDVIHDIEVLLATKRKPKNSK"
     gene            complement(1272..1346)
                     /locus_tag="PK717_01085"
     tRNA            complement(1272..1346)
                     /locus_tag="PK717_01085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1312..1314),aa:Asn,seq:gtt)
     gene            complement(1457..2170)
                     /locus_tag="PK717_01090"
     CDS             complement(1457..2170)
                     /locus_tag="PK717_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR46300.1"
                     /translation="MTDRKLHNRILIIEDDREIARLVELRLKSEGFEVLHSETGQDGL
                     FKFSQNEPDVLILDLMLQDMDGIEICRQVRKRSNTPVLMLTAKSSEVDKVLGISSGAD
                     DYLTKPFSLNELVARIRGLIRRCHYLSAPAKSKEEIVFDDMVVDSKAHSVLKGNRRIH
                     LTVKEFELLWLLMSNPGRVFTREELFTKIWKKQSLVDCRTVDVHVNKLRKKIEDKSFS
                     SSMIRTIHKVGYCFDPGGS"
     gene            2410..3666
                     /locus_tag="PK717_01095"
     CDS             2410..3666
                     /locus_tag="PK717_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR46301.1"
                     /translation="MVENMEDKKNKRAITKIVDFFGLKKSIVGMLSMVVLVGVGEHMA
                     EKFLPLYMVFLGGGFISVGVLNFMDNFLGAIYSFPGGYLADRMGTKKSLLVFNLIAIA
                     GYSLVIIFPYWPVVLIAATLFLSWTAISLPATMGLVSKALPKNKRTMGVSLHSLVRRL
                     PMIFGPMLGGLLIKIYGTELGVRYSFTVALILALVAAVLQQILIEEDNGKKKEEMTSS
                     GLRYLYKLMSPELKKLLVSDILIRICEQIPQAFVVLYVVGAAGKVGITDSLGFGFLTA
                     IEMVTAILIYIPVAYFADKTRKKPFVVATFAFFTLFPVILLGSKSFPMLVAAFIIKGL
                     KEFGEPTRKALIMDLAPENHKAGMFGLYYLIRDLIVSVFAFLGGILWMINPTLNLITA
                     ASFGVLGTLWFAFYGSDLPSAQTGIP"
     gene            3785..4312
                     /gene="raiA"
                     /locus_tag="PK717_01100"
     CDS             3785..4312
                     /gene="raiA"
                     /locus_tag="PK717_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOR46302.1"
                     /translation="MRIQVTGRNVTVGDELSSYAQEKIGKLERYFDGIQDAQVLFTEQ
                     KTVKICEVTMKASGKIIRAEYRGSEFLEAVDFVAEKLERQIRRYKEKFQERRKEGSKD
                     VEPVFVKQEQASQSKIVRVKEVPVKQMNFEEAIMQMELLSHDFFVFYNPDSKRLNILY
                     RRKDGDLGILEPIEA"
     gene            4309..4788
                     /locus_tag="PK717_01105"
     CDS             4309..4788
                     /locus_tag="PK717_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46303.1"
                     /translation="MMKLRELVKKLNFWDSIIVGFVVLTVGLFGYSVYVKAVQPPAKT
                     IEIKVKAGNLYQEVAYSISAGDFIVDRDGRPFFKISQIDELRNAKQAVVKWDGQMISA
                     DNPELYSVEFTAQSVNPKFKGSNLFYNWELVKPGRTMYMETEKTGFMAVIISVGEEK"
     gene            4792..7371
                     /gene="secA"
                     /locus_tag="PK717_01110"
     CDS             4792..7371
                     /gene="secA"
                     /locus_tag="PK717_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR46304.1"
                     /translation="MSILSKLFDSSEKFLGRARPLVSRISALEPEYEKLSDEEIRARI
                     SEIRQQVANGQRLDDVLIPTFAFVREAAKRTLSQRPFDTQMIGGMVLHSGRIAEMKTG
                     EGKTLTATFAATLNALEGKGVHVVTVNDYLAKRDREWMGKVYEFLGLTVGCIYHDASY
                     DERRKSYRSDITYGTNNEFGFDYLRDNMARHVSQLVQRELHYAIVDEVDSILVDEART
                     PLIISGPSEESTDLYEKADKVVRQLREEDYNINEKDKNVTLTEAGVERAEHILNVSNL
                     YGPENVEVNRHISQSLKAHHLFKNEVDYIVKDGEVIIVDEFTGRLMFGRRYSDGLHQA
                     IEAKEGLKVREENQTLATITFQNYFRLFDKLAGMTGTAKTEEDELREIYGLEVVVVPT
                     NKPMIRKDLPDIIWKTEDGKYKHIIDEIVDCYKRGQPVLVGTRSIEKSEKLSSMLHKK
                     MIPHEVLNAKYHEKEAAIIAKAGQMNAVTIATNMAGRGVDIALGPGVVELGGLHILGT
                     ERHESRRIDNQLRGRSGRQGDPGSSRFHLCLDDELLRIFGGERLKSMFNFLKIEEDMP
                     LEHPLLTKTIENAQRKVETHNFDIRKYVLEYDNVLNKQREIIYAERQKVLHGEDLIET
                     VLANLDRACEDMVANHASTELAVQDWDIKTLHSELMDLTAGVVRDVEFKDRQELLENL
                     KESVKTAYNDRETRFGHETMREIERFVLLYVVDQKWKDHLYNMDILREGIGLRSYAQK
                     NPVQEYQNEAFLMFQRMLNSIWEDTIKYLFRVELQADSNQQQKQTRTRTNEDDIKTAP
                     AKSSRGDEPERQGETSRTMRPSSGGHTANEPIRRHGDKVGRNDPCPCGSGKKYKHCCG
                     AGQ"
     gene            7393..8488
                     /gene="prfB"
                     /locus_tag="PK717_01115"
                     /pseudo
     CDS             7393..8488
                     /gene="prfB"
                     /locus_tag="PK717_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            8485..10347
                     /gene="selB"
                     /locus_tag="PK717_01120"
     CDS             8485..10347
                     /gene="selB"
                     /locus_tag="PK717_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOR46305.1"
                     /translation="MTKRSFIVGTAGHVDHGKSTLVTRLTGTDPDRLPEEKARQMTIE
                     LGFAKLDLPSGRMCGIVDVPGHEKLVKQMLMGAQGFDLVMFTVACDEGPKRQTYEHLS
                     ILDMIGIRVGVIVLTKCDLPHDDAVLQSQLDSMLTNTSLEAMPRIKVSAYTGEGIDEL
                     RQTIDDLLDHAIPRPLNMPAFYPVDRVFTVKGFGIVTTGTLWQGQVKKGEELEIFPSR
                     KKGKLRGIESFDAQIETGDAGSRLALNFQNLDKEDISRGNILATPGRFDPTTEFDAKI
                     RLIEKVKRRTRLKFHYATIEQEVDYSDIGGGFARIKLEGPLPLTKGSRFILRSIAPAD
                     TVGGGEVVDLHPEGKLNAPETLQRLENIGQIDDESYLSTMVFERRKSGFEVSRLRGLV
                     NWPDDRFALFLKNGPHKTISGNLYDNNVFDSSSAKLSKLLTDFFKSNPSRNSISKSEA
                     KAKLMPEINENLFSEILLTAIKEPLHMIGQDISLSNQTIQKSQTEVGIERELLREMFS
                     PPTLSELRKIPAFSRNLRETEAAISKMIAEKIIIKSVDDLFFHKKAVVKAYLIMKALI
                     EKDGSAKLAQYRDEVGTTRKYAQAMLELLDALGLTRRVGETRVLGYKTLDIGGK"
     gene            10372..10463
                     /locus_tag="PK717_01125"
     tRNA            10372..10463
                     /locus_tag="PK717_01125"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10406..10408,aa:Sec,seq:tca)
     gene            10483..11133
                     /locus_tag="PK717_01130"
     CDS             10483..11133
                     /locus_tag="PK717_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOR46306.1"
                     /translation="MNIIVIGCGRVGSALATILSVDGHNVSVIDEDESAFDKLPQSFC
                     GKKVVGIGFDSQTLIEAGIEKTDAVAIVTPSDNTNIMVAETVRSVFGVKNVVARIYAP
                     RKKRTYDRLNLRSVCPTTLGAYQIYAELLSSMVESKLPLNNGDFELLEIPASSVDEEE
                     TRSFEKESGSKIVGARTDTRVVFPWLGEKLDDVRSWIILAPTSHLRSIVDSIVGGA"
     gene            11139..>11235
                     /locus_tag="PK717_01135"
     CDS             11139..>11235
                     /locus_tag="PK717_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOR46307.1"
                     /translation="MIIVGGGKVGSNLAKILIAHKHQVAVIEADED"
BASE COUNT         3402 a         2407 c         2657 g         2769 t
ORIGIN      
        1 atcaaaaagt accagtaaaa ctctctgatg aaaacggttg gggcagatct aggagctata
       61 aaaccagtta gccttttgaa ctcctggaat tgaagccgta gcttctttct tcctgtatgg
      121 tcccatcaag cttgtgcact ctcaaggagc caccaccatg gttttttata taatcggagg
      181 cttttttcaa aagatcgtct tttgtcgacg catgcatcat aactttttct gtatcaacct
      241 ttttaacaat ccatttcttt tctttgtccg gaacgagatg gtactgagta tgttttacac
      301 tcgcattgtg tgccacttgt aaagctggct ttgtagaagt ttttgttttc ctttttaaaa
      361 ctctgctttt ctggaccatt accccctcct ttggagtgat atgctagatt gtacacatct
      421 gacagggaca agttgttacc gaaaaatcac ttattgattc acgccaaatc ttttgctaga
      481 ctctgtccgt gttttgaata aaactgcaaa aaccactctt tcttgtattt ttgtgataaa
      541 tcatacaaaa agatcattga tatgccagta ttttaattgt gacaatttta tggtgcatgg
      601 ccacaagaaa caatctgcta ttaatactat ttgtatccag aaattttatt acaatgttga
      661 caaggagagg tgcccaaatg gaagagtctt ttttgaaact aattgaccca aatgatgtac
      721 cagacaccaa caaaattgaa aaattcttag gcaaaacatt tgttttctgg aaacagatat
      781 cagacctgat tgatcaaaaa taccctggta ttttcacccc tgaatggtta tatgctggca
      841 aaaaacacgg ttggtcactt cgatacaaaa agaataaatc attctgcacg atgattccag
      901 gaaaagatcg actagccatt cagatcgtat ttggcaaaga tgagcgttcc aaggttgaag
      961 aaataaaaga aaatttgtcc agcagaacaa aaaatgccta cgagagcgca acaacatacc
     1021 acgatgggaa gtggctcttg ctgaatgtcg atggcgaaga tgtcatccat gacatagagg
     1081 ttcttcttgc cacaaaaagg aaaccgaaga attcgaaatg aaatgcttgt gtttgtttca
     1141 atggctggca aaatgcaaca cgaaaagtcg ctggccaatt attaatgtat ttttcttaca
     1201 aaaaaatggg gttatctttt tggggaataa atattgtgac cgatgagcaa ttgaggaact
     1261 cctactagta ctggctcccc gacgaggatt cgaacctcga acctactggt taacagccag
     1321 ccgctctgcc gttgagctat cggggagtag cgtagggcat tatatccacc gagggcacca
     1381 tgtcaagaat gaaaggttat aaaacaatta ttggcatgaa cgcgagcctc tggcagcttt
     1441 atttcgaccc tcaggttcag cttcctcctg gatcgaaaca gtatcctact ttgtggattg
     1501 tccttatcat tgaggatgaa aacgacttgt cttcaatctt ttttctgagc ttgttcacat
     1561 gaacatcgac tgtcctgcaa tccacgagag actgtttttt ccaaatcttg gtaaaaagct
     1621 cttcgcgtgt gaaaaccctt ccagggttac tcatcaggag ccacaacagt tcgaactcct
     1681 tgacggttag atgtatgcgt ctgttgcctt tgaggacaga gtgtgccttt gaatcaacaa
     1741 ccatgtcatc aaaaacgatt tcttcttttg attttgctgg ggcagaaaga tagtgacagc
     1801 gtctaataag accccttatt ctggcaacca attcgttaag ggaaaaaggt ttggtcaaat
     1861 aatcatcggc acccgatgaa atgccaagga ctttgtctac ttcagagctt tttgctgtga
     1921 gcataaggac aggggtattt gaacgcttcc tgacctgcct gcatatttca ataccatcca
     1981 tgtcttgaag catcaggtcg agtatgagaa cgtcaggttc attctggctg aatttaaaaa
     2041 gaccatcttg ccctgtttct gaatggagga cttcaaatcc ttcggattta agtctcagtt
     2101 ctacaagtcg ggcaatctcc ctgtcatcct cgataattaa tatccgatta tgtaactttc
     2161 tgtccgtcac atgtataaat ttatgttaca ataataagat gtcaagtgac cgtcgatatt
     2221 tctttgtttc tgcaagattt taataaatat gactacttag atacctggca aagcatataa
     2281 tcactctgcc atttttaggc caagcatgaa caatccaata tttggcctac cttgacaaaa
     2341 tgactgtaga aaattgatca agattttgtg agagccgcac cacacctata acctcacagg
     2401 acaggataaa tggtagaaaa catggaagat aagaaaaaca aaagggccat taccaaaata
     2461 gtggattttt ttggtcttaa aaaaagcatt gttgggatgc tttcgatggt tgttctggtt
     2521 ggggtcggcg agcacatggc agagaaattc ctgccgctct acatggtttt tcttggtggt
     2581 ggtttcattt cagttggtgt tctcaacttc atggacaatt ttcttggggc catctattca
     2641 tttccaggcg gctatcttgc ggacagaatg ggcacaaaga aatccctcct tgttttcaat
     2701 ctcattgcaa tagctggata ttcgcttgtg ataatttttc cttactggcc ggtagttctg
     2761 attgcggcaa cgctttttct ttcatggaca gccatatcgc ttccagccac aatgggcctg
     2821 gtctcaaagg cactgccaaa aaacaaaaga acgatgggtg tgtcgttaca ttcgctcgtc
     2881 agaagattgc caatgatatt tggcccaatg cttggcgggt tgctgataaa aatatatggc
     2941 acggagcttg gtgtaagata ctcattcaca gtggcgctaa tacttgcgct ggttgccgct
     3001 gtgctacagc aaatcctgat cgaagaggac aacggcaaaa agaaagaaga aatgacttct
     3061 tcgggtctca ggtatttgta caagctgatg agcccagaat tgaagaaact gcttgtttca
     3121 gatattttga tcaggatttg cgaacagata ccacaagctt ttgttgttct gtatgttgtt
     3181 ggggctgccg ggaaagttgg cataaccgat tcgcttgggt ttggtttttt gactgcaatc
     3241 gaaatggtaa cagcaatcct tatctacatt cctgtcgcct actttgctga caaaaccaga
     3301 aagaaaccat ttgttgtggc aacctttgct ttcttcactc tgttcccggt cattctcctt
     3361 ggctcaaaat ctttcccgat gctcgttgct gcatttataa tcaagggact caaggaattt
     3421 ggcgaaccaa ctaggaaggc gcttataatg gatttggcac cggagaatca caaagcaggg
     3481 atgtttggtc tctattacct tataagagac ttgatcgtgt ctgtcttcgc gtttctaggc
     3541 ggaatactgt ggatgataaa tccgactctt aatctcataa cagcagcatc tttcggggtt
     3601 ctcggcacac tctggtttgc tttctatggg tctgacctgc cctctgcgca aactggtatc
     3661 ccctgaagcc aaataatctt gcaatcacaa tgtttctttt cacaaaatca ttttggtttt
     3721 gcttgacttt atcccccgtg acgtgataat tatttattca aggtcacgaa aggaggcaca
     3781 caatatgcgt atacaggtaa ccggtagaaa cgtaacggta ggcgatgagc tcagttcgta
     3841 cgcgcaggag aagattggaa aactcgagag gtatttcgat ggcattcagg acgcccaggt
     3901 tcttttcacc gaacaaaaaa cagtcaaaat ttgtgaggtg acgatgaaag cgtccggcaa
     3961 aatcatccgt gccgagtaca gaggctcaga gtttttggaa gcggtagact tcgtggccga
     4021 gaagcttgaa aggcaaatcc gccgttacaa agagaagttc caggagagga gaaaagaagg
     4081 ctcaaaagat gtagaaccag tttttgtgaa acaggagcag gcgtcacagt cgaagattgt
     4141 aagggtaaaa gaagtgccgg taaaacagat gaacttcgaa gaagcaataa tgcagatgga
     4201 attgctgtca cacgacttct tcgtgttcta caatcctgat tccaagaggt tgaacatcct
     4261 ttacagaagg aaagacggcg atttgggaat ccttgagccg attgaagctt gatgaagtta
     4321 agagagcttg ttaagaaact aaatttttgg gatagcataa tagttggttt tgtagtgctg
     4381 acagtgggtc tgtttggata cagcgtttat gtgaaagcgg tccagccacc agcaaaaaca
     4441 atcgaaatca aagtaaaggc tggcaacctc taccaggagg ttgcctactc catctctgcc
     4501 ggagacttca ttgtggacag ggatggaagg cctttcttca aaatcagcca gattgacgag
     4561 ctgaggaatg caaaacaggc tgtggtcaaa tgggatgggc agatgatttc tgctgacaat
     4621 cccgaacttt actctgtcga attcaccgcc cagtctgtaa accctaaatt caagggaagc
     4681 aatttatttt acaactggga actagtcaag cccggaagga ctatgtatat ggagacggag
     4741 aaaaccgggt ttatggcggt aatcatctca gttggtgagg aaaaatagta aatgagcata
     4801 ctgtcgaagt tattcgactc gtccgaaaag ttcctcgggc gggcaagacc acttgtgtca
     4861 cgaatcagcg cactggaacc agaatatgaa aaactttccg acgaggaaat aagggcaaga
     4921 ataagcgaaa taaggcaaca ggtcgcaaac ggccagaggc ttgatgatgt acttattcca
     4981 accttcgcat tcgtaaggga agccgcaaaa aggaccctca gccaaagacc atttgacaca
     5041 caaatgatcg gtggaatggt cctccactcg ggaagaatag ccgaaatgaa aaccggtgag
     5101 ggaaaaaccc ttaccgcaac ctttgccgca acacttaatg ccctggaagg caaaggggtc
     5161 catgttgtta ccgtaaacga ctaccttgca aaacgtgaca gggaatggat gggcaaggta
     5221 tacgaattcc tggggctaac cgtaggctgc atctaccacg acgcgtcata cgacgaacgc
     5281 aggaaatctt atagaagcga catcacctat gggaccaaca acgagtttgg ttttgactac
     5341 ctcagggaca acatggcaag gcatgtttcc cagcttgtcc aaagggaact ccattacgcc
     5401 atcgtcgatg aagtcgacag cattctcgtc gatgaggcaa ggacaccgct cattatttca
     5461 gggccttctg aagaatccac cgacctttac gagaaagccg acaaggtcgt cagacagctc
     5521 agggaagaag actacaacat caacgaaaag gacaaaaacg tcaccctgac cgaggctggt
     5581 gttgaaaggg ccgaacatat attgaacgta agcaaccttt atggtccaga gaatgttgaa
     5641 gtcaacaggc acatcagtca atcattaaag gcgcaccatc tcttcaagaa tgaagtcgat
     5701 tacatcgtaa aagatggtga agtaataatc gtcgacgagt tcaccggaag gttgatgttc
     5761 ggcagaagat acagcgatgg cctccatcag gccatagaag caaaggaagg tctgaaagtg
     5821 agggaagaaa atcaaaccct tgccacaata accttccaga attacttcag gctgtttgat
     5881 aaactggccg gcatgacagg aaccgcaaaa accgaggaag atgaactaag ggaaatctat
     5941 gggctcgaag ttgttgttgt accaaccaat aaacccatga taagaaaaga cctcccggac
     6001 atcatctgga aaacagagga cggaaaatac aagcacatca ttgacgagat agtggattgc
     6061 tacaaaaggg gccagccggt ccttgtcgga acaaggtcaa tcgagaaatc cgagaagctc
     6121 tcgtcaatgc tccacaagaa aatgatcccc catgaagtgt tgaatgcgaa ataccacgag
     6181 aaagaagctg cgataattgc caaggcaggc cagatgaacg ccgtgaccat agccaccaac
     6241 atggccggtc gtggcgtcga catagcgctt ggccctggag tggttgaact cggcggcttg
     6301 catattctgg gaactgaacg ccacgaatca agacgtattg acaaccagct caggggaagg
     6361 tcgggcaggc agggtgatcc gggttcatca aggttccacc tttgccttga tgacgagctc
     6421 cttagaatct ttggcggtga aagactcaaa tcgatgttca acttcctcaa gatagaggaa
     6481 gacatgcctc ttgagcaccc acttctcaca aaaacaatag agaatgccca gcgcaaagtt
     6541 gaaacccaca actttgacat aagaaaatac gttttggaat atgacaacgt actcaacaag
     6601 caaagagaga taatatatgc ggaacgccag aaggtactgc acggtgaaga cctcatagaa
     6661 accgtgcttg caaaccttga cagggcctgt gaggacatgg tcgcaaacca tgcctccaca
     6721 gagcttgccg tgcaggactg ggacatcaag acactccact cagaactcat ggacctgact
     6781 gccggtgtgg tcagggatgt tgagttcaaa gacaggcaag agctccttga gaacctgaaa
     6841 gagtctgtca agaccgccta caatgacagg gaaaccaggt ttggtcatga aacaatgcgc
     6901 gaaatcgaga gattcgtgct gctctatgtg gttgaccaga agtggaaaga ccatctctac
     6961 aatatggaca tcctcaggga aggaatcgga ttgaggagct acgcgcagaa aaatccggtc
     7021 caggaatacc agaatgaggc attcctgatg ttccagagga tgctcaactc gatttgggaa
     7081 gacacaatca agtatctgtt cagggtagag ctccaggccg actcaaacca gcaacagaag
     7141 caaacaagaa cacgaaccaa tgaagatgat ataaaaaccg ctccagcaaa atcatccaga
     7201 ggcgatgagc cagagagaca aggcgagaca tcaagaacaa tgagaccttc gtctggcggt
     7261 catactgcca atgagccaat aagaaggcat ggtgacaaag tcggcagaaa tgatccgtgc
     7321 ccctgtggtt caggaaaaaa atacaaacac tgctgcggtg caggacaata gcagacaggc
     7381 aaggaaacaa gaatgttgat agacgaaata cagaggtcca tagatcaggt aaaagaacgg
     7441 ctcaaccagg ccaaggagtt tctttgaccc gctaaaggct gaacgggaaa aactttcaaa
     7501 acagctggag tctccagagg tctggaatga ccagcaaaaa attgccgaga ccaacaaaaa
     7561 aattgccata ttcgatatca aactcaatga cctggaacag gccgaacatt tcattggcga
     7621 agccgaagtt tacatggagt ttgtccagat gggcgagggc tcagagaaag agcttcttgg
     7681 cgcaataaaa caggccgagg caaagataca ggaaatagag ctcaaaaacc tcctctccat
     7741 ggagcatgac cagctcaatg cctttgtttc attcagctcc ggtgcaggcg gagtcgacgc
     7801 ccaggactgg actgaaatgc tcatgaggat gtacacaagg tgggcaacca acaagggttt
     7861 caaggttact gttgctgaat acagccctgg cgatgaggct ggtgtcaaat cagcaaccct
     7921 ccacatcgag ggcccgtatg cctttggttt cctcaagagc gaaaagggtg tccacagact
     7981 ggtcaggatt tccccgtttg acgctaacca cagaaggcac acctcatttt cactcgttga
     8041 ggtgctcccg gagctcccgc ctgcagaaga tattgaaata aaccccgatg atttgaggat
     8101 tgacacgttc aatgcttcag gccacggtgg tcaaaacgtc cagaagaacg atactgctgt
     8161 caggataacg catataccaa ccggccttgt ggtggcatgc cagaatgaac gttcacaggg
     8221 tctcaacagg gccatggcca tgaaaatcct ctcatcaaga ttacaggacc tggcaaatgt
     8281 ccagcttgca gaggaagaga aaaaactcag ggggccaagg gtctcaattg agtggggcaa
     8341 ccagatacgc tcctatgttc ttcagcctta cactctcgta aaagacaaca ggacaggcta
     8401 tgaggtagga agtgtcgaca gagttcttga gggtgacctg gatgaactta tctgggccta
     8461 cctgaaatgg gcggtaaaaa acaaatgacc aaaaggagct ttattgtcgg aacagcagga
     8521 cacgttgacc acgggaaatc aacccttgtc acaagactga ccggtactga tcccgacagg
     8581 ctccctgaag aaaaagccag acagatgacg atcgagcttg gttttgcaaa gcttgacctc
     8641 ccctccggaa ggatgtgtgg aatcgttgat gtgccaggcc acgagaaact ggtcaaacag
     8701 atgctcatgg gcgcacaagg gtttgacctg gtaatgttca ctgttgcatg tgatgaagga
     8761 ccaaaacgcc agacctatga acatctatca atcctggata tgattgggat ccgggttggt
     8821 gtcattgtgc tgacaaaatg tgatttgccc cacgacgatg cagttttgca atcacaactc
     8881 gattcaatgc ttacaaacac ctctctcgag gcaatgccaa gaataaaggt ttcagcctac
     8941 actggggaag ggatcgacga actcaggcaa actatcgatg atctgcttga ccatgctatc
     9001 ccaagacctc ttaatatgcc agcgttctat ccagttgata gagtatttac agtcaaggga
     9061 tttggaatag tgaccaccgg caccctctgg cagggccagg tgaaaaaggg tgaagagctg
     9121 gagatattcc cttcaaggaa aaaaggcaaa ttgagaggga tagagagttt tgatgcccag
     9181 attgaaactg gtgatgctgg ttcaaggctt gccctcaatt ttcaaaacct cgataaggaa
     9241 gacatttcaa ggggaaacat tcttgccacc cctgggagat ttgacccgac aacagaattt
     9301 gatgcaaaaa ttcgcctcat tgaaaaagtg aaaaggcgta caaggctaaa gttccattat
     9361 gcaactatcg agcaggaagt tgattacagc gatataggag gtggttttgc aagaataaaa
     9421 ttggaagggc cgctccccct cacaaagggt tccagattta tcctgaggtc gattgccccg
     9481 gcagatacgg ttggcggtgg tgaagtggta gacctccacc cagagggtaa gctcaatgcc
     9541 ccggaaacgc tccagagact tgaaaatatc ggccagattg atgatgaaag ctatctttca
     9601 acgatggttt ttgagcgtag gaaatcaggt tttgaagtgt ccaggctcag agggcttgta
     9661 aactggccag atgacagatt tgcattgttt ctcaaaaatg gcccccataa gacaatatcc
     9721 ggcaatctct atgacaacaa tgtttttgat tcatcatcag caaaattgag caaattgctg
     9781 acagatttct tcaaatcgaa cccgtccagg aattccattt caaaaagcga ggcaaaagcc
     9841 aagctgatgc cggagataaa cgagaatctt ttttcagaaa tccttttgac tgccataaaa
     9901 gagccattgc acatgattgg tcaggatata tccctttcaa atcagaccat acaaaaatca
     9961 caaaccgagg ttggaataga aagagaactc ctgagagaga tgttctcccc tccaactctc
    10021 tcggagctca gaaaaatacc tgctttttcc agaaacctgc gtgaaaccga agcagcaatt
    10081 tcaaaaatga tcgcggagaa aataataatc aaatctgtag atgatctttt ctttcacaaa
    10141 aaagctgtgg taaaggctta tctaataatg aaagcactca tagaaaaaga tggttcagcc
    10201 aaactcgcac agtacaggga cgaagtaggt acaactagaa agtatgccca ggcaatgctg
    10261 gagctacttg atgcccttgg cttaacaaga agagtaggtg aaaccagagt gctcggttat
    10321 aagactcttg acattggtgg taaatagtat tagtgtctcc cttctgatcg cgggagcggt
    10381 tgggctctgg tgggcctcct ggacttcaaa tccagtgtgg ggtgaactcc ccgggtaggt
    10441 tcgattccta tgcgctcccg ccaattacac tagaggtgaa gcatgaacat cattgtgatt
    10501 ggttgtggaa gggtcggttc ggcgcttgca accatattgt ctgtggacgg tcacaacgtt
    10561 tcagttatag atgaagacga gtctgcattt gacaaacttc ctcaaagttt ttgtggtaaa
    10621 aaagtcgttg gcattggttt tgattcacaa acgctcatag aagcagggat agaaaaaaca
    10681 gatgcagtcg caatcgtgac cccatctgat aataccaaca tcatggttgc agaaaccgtg
    10741 aggtcagtct ttggcgtaaa aaatgttgtc gcaagaattt atgcacccag aaagaaaaga
    10801 acctatgaca gattgaatct aaggtctgtt tgcccgacaa ctttgggcgc ctaccagata
    10861 tacgcagaac tcctcagttc catggttgaa tcaaaattgc cactcaacaa tggcgatttt
    10921 gagctgcttg agataccagc atcctcagtg gatgaagaag aaacgaggag ttttgagaag
    10981 gaaagcggat caaagatagt cggcgcaaga acagacacaa gggttgtttt tccctggctt
    11041 ggtgaaaaac ttgatgacgt tagatcgtgg ataatccttg cacctacaag tcaccttcgt
    11101 tcaatagttg attcgattgt gggaggtgca tgatgtatat gattattgtt ggtggaggga
    11161 aagtaggctc caatctggca aaaattctca ttgcacacaa acaccaggtt gccgtcatag
    11221 aggccgatga agatc
//
LOCUS       DAOSRI010000020        11025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5828_length_11025_cov_4.361531, whole genome shotgun sequence.
ACCESSION   DAOSRI010000020 DAOSRI010000000
VERSION     DAOSRI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5828_length_11025_cov_4.361531"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PK717_01140"
     CDS             complement(<1..1119)
                     /locus_tag="PK717_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR46308.1"
                     /translation="MKNLDDLIREKDELLAKASNETPQAREFQEKRGKLTARDRVKLF
                     LDPESFVETDTLVQHRKDYPQSEKFVPGEGVITGYGTVNGRLIFVFSQDFSIMGGSLS
                     KMQGAKIGKVMEMAYRSGAPVVGFNDSGGARVQEGVDALSGYGKVFYRNSIYSGVIPQ
                     IAIIAGPSAGGAAYSPALTDFIFTIDGLTEMFITGPQVIESVTGEKPDKQTLGGARIH
                     ASKSGVTHFIYKSEQECIQSVRRLLSFLPQNCRQMPPRIKGNDDPNRASMALRQIVPV
                     EANKSYDVRKVIAEIVDDGDFMEVQPDFARNLTIGFARVDGFPIGIVANQPMYLAGCL
                     DIDSSDKASRFIRTCDSFNIPLLNLVDVPGFLPGVNQEH"
     gene            1434..1988
                     /locus_tag="PK717_01145"
     CDS             1434..1988
                     /locus_tag="PK717_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46309.1"
                     /translation="MNTNPEVTEYLILAYKGELAIKSADVSKVRQWALIACEAIATKY
                     KNACKQYLVNHPKAKEAKISRDFVDAFFDAIGATMNKEYSFELPEGAPTMNQAIKAAR
                     ILSAHAYELQSFPRFAWGLASIVFGMLFVASAFSLWFSRDFSGWAIFVSTLLFMIYCI
                     YNWRQFENFEKASREIKDRVERSK"
     gene            2048..2767
                     /locus_tag="PK717_01150"
     CDS             2048..2767
                     /locus_tag="PK717_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR46310.1"
                     /translation="MNPYYGCSHACSFCYATFMCRFLDEKERWGRFVYPKINLPDVLM
                     KQVKKAGGKSVVFSTATDAYQPLEAKFGVTRACLELLSKVDCRVDILTKSALVVRDVD
                     LIAKFDGPTVGFSMSYHDERLRAAFEDDSAPILARIDALKMFKQRGVSTWIFMSPILP
                     LVTNVDRIIEIAATIGCGLSMDFLNPYPRVLRSLAISYGKLGLNFDDAMRLLSNADFR
                     KAQKLRAIEKANRIGVQLTCY"
     gene            complement(2773..3024)
                     /locus_tag="PK717_01155"
     CDS             complement(2773..3024)
                     /locus_tag="PK717_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR46311.1"
                     /translation="MENRKIGWLPVIVVLIIGVPSLWIGIDKSNVAFIIFGAFELVVG
                     IIGIWAILRNSKVPRTICPNCKEPIGPGMESCPNCGIKL"
     gene            3098..3616
                     /locus_tag="PK717_01160"
     CDS             3098..3616
                     /locus_tag="PK717_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46312.1"
                     /translation="MVEFIKMRKIICFMLLVAVVAGCGANVPTWDEINAMVDGGEYKS
                     KIDEAIKQAGPENHPASKVVISYFENLFSGKYDEAWSQIEKNSPFQTARVSKEQMVEM
                     FEKAKAEVDYKSARVISLSIMKKNANVRMVKVGFMLYVYDKLKKQDDTPIGSYTLSDL
                     TGEWKIFDSEIK"
     gene            complement(3705..3872)
                     /locus_tag="PK717_01165"
     CDS             complement(3705..3872)
                     /locus_tag="PK717_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46313.1"
                     /translation="MSKITLIIVGILLVIMGLSGIWSWFGLGVEPMWHAILKIAIGII
                     AVLIGAMDKTA"
     gene            complement(3982..5799)
                     /locus_tag="PK717_01170"
     CDS             complement(3982..5799)
                     /locus_tag="PK717_01170"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOR46314.1"
                     /translation="MEPYSDSKPLTRWWWFSGPINEKDIDFQLSWLKNNGFGGVEIAW
                     VYPLPESPEGPAWLSRELSDKIRHTKERARSLGLACDFTFGTLWPFGGSMVSEEDASK
                     DFYGTSKQRIRKSWEARTYPETFVLDHMNEGAFLRYAEKMGNFLPLDGKKQCLFCDSF
                     EVATEDLWAKGFENDFKSAFGYDIVPFMDDLDSSPGARYDYRKLVSEMLLENFFSPFA
                     RWCREHNSFSRVQVHGAPVDILEGYATCDVPESEAVLFDPHFSQLAASSAALAGKKYV
                     TAETFTCLYGWKGWPGPGQHQREEKTGDLKLLADAMLANGTNKVIWHGMPYNPEGGKN
                     SFYASVHVGPDASFAEDLPALNGYMKKISDFLLEGKPYFQCAVMLPVEDAWMEGELED
                     KLKRPSAKYHWEFHYQRMSEELWGHRPCWITAPFLKRAEVRDGFTVIGNLEFDFIYVD
                     CQYMDFEELDALISLAQKGARICLKQIPEQPGFIASPAYEKAITKLASFENVSDNIKK
                     SSTLLPLFTATNKLKPEYWCRKTGDGINVFMANPATRAISYPMPYGLSDGTTEVKMEL
                     DAAFLGQKKTIDISFKKGASIMLQLSKGGIICEETNLLN"
     gene            complement(5818..6612)
                     /locus_tag="PK717_01175"
     CDS             complement(5818..6612)
                     /locus_tag="PK717_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46315.1"
                     /translation="MKTPISIPFPASEKPKLKFSIGACKFNLRPGDGTSWVEGTYDDP
                     TDNLPCIIEQSGNEVSVSQSRNISGLINVFNRTPTFDLVLSSQNPFDLVIEGGASENC
                     IELGGLPLSSVAVSFGAGKFEMSFLKPNPILMDSFRVSTGAGSMTLSKLLNAGFKNLK
                     VEGGAASYKIDFTGKLGQDCNANIQIGVASLDLDVPSDVPVAVKSETTLGGVEMGSGF
                     TTKDGRLVNQAYLANPSPALSISTSIALGSLKLRIANSTGGLRAVP"
     gene            complement(6609..6866)
                     /locus_tag="PK717_01180"
     CDS             complement(6609..6866)
                     /locus_tag="PK717_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOR46316.1"
                     /translation="MPEKCRTEEFQVSGEKVIEKIKELVHEGNIRRIIIKNDHGSTLI
                     EIPLTLGVIGAVLLPVWAAIGAIAALVTKMTIVVERVEEEQ"
     gene            6956..7348
                     /locus_tag="PK717_01185"
     CDS             6956..7348
                     /locus_tag="PK717_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOR46317.1"
                     /translation="MKKILALACVAILFAGCGGAAAGPTKVTEEDKGKTFEATVGQKF
                     EVTLKANETTPVQWTGPKIEGDAIIGTVNSEYKTEENPEGMVGVGGWRIYTFEVTAAG
                     TAKMVWESKHIAEEGPPYDTFEITINAK"
     gene            7398..8774
                     /locus_tag="PK717_01190"
     CDS             7398..8774
                     /locus_tag="PK717_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46318.1"
                     /translation="MRDKLVIIILVVMGLAGQFAPACLSGSNEIRLEGVLYTKNTEKN
                     LFILKSGDCLYKFSPKNANEMERLRSGDSLVLTAIQDANRKFVLHDATFKIVYDKTVT
                     TVLWAKLSSGGWHDGLFRVLTSNDEYWQVNFISGEGTAGLKSETYYTFRGAQDHHLSQ
                     TINNASVYDTVRRKISLLKVVSVDAKTGWLETKNTSDNDIRVYSTDAAENLANMKKDD
                     FIRVEGYQDVKDSYGPILFASICTKNPCPETVSVEFVGEVLDVPSKINGNKYSIRECD
                     SVINFEYDGPNGKSSIGNWVKVKGSYSLKERLCIPSSITLTNPAIICTVFERDPVSRD
                     LRGIEPEVNIRWNIQLSEKSRSKSVPLGTMLKVSSSSYLQRRLIGCKTSDVVKISGMI
                     IDIDYELQTATIESVAGEKTTTHIRPEVVDLLSFNYEDQVEVYGLHATKSSQEGHIFE
                     CVLKKASK"
     gene            8881..9999
                     /locus_tag="PK717_01195"
     CDS             8881..9999
                     /locus_tag="PK717_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46319.1"
                     /translation="MSTEIIEVSQKRELKEFIHAPTKLHRGHEGWATPLYSDEWKFFS
                     RRKNKNFNHSDTVMVLAKKRGRIVGRCMGIINKRFLEISGEKTARFGYLESTDDIEVT
                     KALVTHVEKWAKSRNMNKIVGPMGFTDQDPEGFLIEGFGNEPTLATYYNYEFIPEHIE
                     KLGYGKEVDYVVYKIPVTSEYPPIYKKVLERLSKKNEFKLVEFSSTSQIKPYIVPVLS
                     LVNDVFSHLYGYVPLAMEEMEDLAKQYLPALNPKFLKVVEKDSKLVGFILGIPNMDEG
                     FRKARGRLLPFGFYHILSASKKSRQLDLLLGGIDREYRDRGIDVLLGSSLMKSAHDSG
                     IKFMDSHLELESNTLVQAEMKRFGGQVYKKYRIFQKKL"
     gene            10062..>11025
                     /locus_tag="PK717_01200"
     CDS             10062..>11025
                     /locus_tag="PK717_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46320.1"
                     /translation="MGRALLVVIVLVSQLLLPVRGLKAEPCFYSHAGSTGCGQARISG
                     EEDKVKELDLEGCPGDKNDLWVDLTVTSGGTASVEVLWLPPDNVSPRDWNGSWMDPTP
                     KQFQLEEDATIKIALNIKWHMLKVGEMSKGRLVFAMTWVIAGKKVTKNLIYKTTMTIP
                     GPKIKAEDVVVKSVCSDGAISKFGIPVENFGCGMKGQIVFGECKFLEKSHIFPVDIAQ
                     REEGKGKSVTMVAIDGLAVRMRDRKVMLPVFIRDLSRTPVIDVPLCSVTLLSNMPPLL
                     EGSQTFSVVRGTKTAFGVACSDEENDPVEFSVLGAPPWCTIEKKG"
BASE COUNT         3049 a         2386 c         2564 g         3026 t
ORIGIN      
        1 atgttcctgg ttgactcccg ggaggaagcc tggaacatcc acgagattca ggagcggtat
       61 attgaaggaa tcgcatgttc tgataaacct cgaagctttg tcggaagaat caatgtccag
      121 acaaccagcg aggtacatcg gctggttggc gacaatgcct atcgggaatc cgtccactct
      181 tgcaaaaccg atggtgaggt ttctggcaaa atctggttgg acttccatga aatcgccatc
      241 atcgactatt tccgcgatga ccttgcgaac atcgtaggac ttgtttgctt ccactggaac
      301 aatctgtctg agtgccattg aagccctgtt cggatcgtca ttgcccttga tgcgtggtgg
      361 catctgcctg cagttttgtg ggaggaatga caggagcctc ctgactgact ggatgcattc
      421 ctgttctgat ttgtaaataa aatgtgtcac gccagatttt gaggcatgga tccttgcgcc
      481 accgagggtt tgtttgtcgg gtttttcacc agttacagat tcgataactt gggggccagt
      541 tatgaacatc tcagtaagcc cgtcaattgt gaagatgaaa tcagtgaggg caggagagta
      601 ggctgcaccg ccagcggatg gccctgcaat gatggctatc tgcgggatga cgcctgagta
      661 tatggaattt ctgtagaaca cctttccata tcctgaaaga gcatccacac cctcttgcac
      721 ccttgccccg ccggaatcgt tgaatccaac taccggagca ccggagcgat aggccatttc
      781 catgactttg ccaatttttg caccctgcat ctttgacaag gaaccgccca tgattgaaaa
      841 atcctgggag aagacaaaaa taagcctgcc gtttactgtg ccatagcctg ttataacacc
      901 ctcccctggg acgaatttct ctgattgcgg atagtccttt ctgtgttgga ccagagtgtc
      961 tgtttcgaca aaactttctg ggtcaaggaa cagcttgacc ctgtctctgg ccgtcaattt
     1021 ccccctcttc tcctggaatt ctctggcctg tggcgtttca ttgcttgctt ttgcaagcaa
     1081 ttcatctttt tctcttataa gatcatctag attcttcaca tcgtcaccat aaccttgcac
     1141 cagggcaagt caattatggg gttgctaatg tcaaacagac gttttattaa atttgtttta
     1201 tttacggtct cacgatgtaa gaggatgttt tgtaaatgtt tttgtgcaca tttgtgaaat
     1261 acattttcta ttatcaaagt tgaaacagat taacagtgaa attggacaca aaatcgattg
     1321 gtttaattct atacgaatta tagtcgaaat aatgcacaga ttaatattta cataaccaaa
     1381 agtgtaaccc attcaggttt gaatctttcc acaaaaaacc ttatccttga gtcatgaata
     1441 caaacccgga agttacagaa tacctgatcc ttgcctataa aggtgagctg gcaataaaat
     1501 ctgctgacgt gtcaaaggtc aggcagtggg cactgattgc ctgcgaggca atagccacaa
     1561 agtataagaa tgcctgtaag caatatcttg tgaaccatcc aaaggcaaag gaagccaaga
     1621 tatcaaggga tttcgtggat gctttttttg atgccatcgg tgcaacaatg aacaaggagt
     1681 attcttttga acttcctgag ggagccccaa ccatgaacca ggcaatcaag gcggccagaa
     1741 tattgtctgc ccatgcttat gaattgcagt cttttccaag atttgcctgg ggtcttgctt
     1801 cgatagtgtt cggaatgctc tttgttgctt ctgcgttctc actctggttc agccgtgact
     1861 tctccggctg ggcgatcttt gtttcaacat tacttttcat gatctattgc atctataact
     1921 ggcgccagtt tgaaaatttt gagaaagcct ccagagagat aaaggacagg gtggagcgat
     1981 caaaatagag gccaggctta caaaatcggc acttgtaaag tcgaaggtcc agggctgtga
     2041 ttactcgata aatccttatt atggttgttc tcatgcgtgt tctttttgtt atgcaacttt
     2101 catgtgccga tttcttgacg agaaggagcg ctggggaaga tttgtatatc cgaaaataaa
     2161 cctgccggat gttttgatga agcaggtcaa aaaggcaggt ggcaaatctg tggttttttc
     2221 gacagccaca gatgcctatc aaccccttga ggcaaaattc ggggtaacaa gagcgtgcct
     2281 ggagctgctt tcaaaggtcg attgcagagt agacatcctg acaaaatctg cccttgtggt
     2341 cagagatgtc gatcttatcg caaaatttga cggaccaact gtcggatttt cgatgtccta
     2401 ccatgacgag cgcctcaggg cagcctttga ggatgactct gcaccgatac tcgccagaat
     2461 tgatgccttg aagatgttta aacagagagg cgtgtcaaca tggatcttta tgtctccgat
     2521 attaccactc gttacaaatg ttgacaggat aatcgagatt gctgcaacaa tcggttgtgg
     2581 cctctcgatg gattttttga atccctatcc gagggtatta agaagccttg ctatttcgta
     2641 cggaaagctc ggtctaaatt tcgatgacgc aatgagattg ctctccaatg ctgatttcag
     2701 aaaagcccaa aaactcaggg caatagaaaa agcaaacaga ataggtgttc agcttacatg
     2761 ctattagtca ccctagagtt tgattccgca attcgggcat gattccatcc ctgggccaat
     2821 tggttccttg cagtttggac aaattgttct tggcactttt gaattccgga gtattgccca
     2881 gataccaata atgccaacaa ccaactcgaa agcaccaaag attatgaatg cgacatttga
     2941 tttgtcgatg ccaatccaga gtgatggcac gccaataatc aaaactacaa taaccggcaa
     3001 ccacccaatt tttctgtttt ccattgcaac ctccttgctg gattatacaa gttttggatg
     3061 ttttggaaac accaccccct tccaaagatg gccataagtg gtagaattca taaagatgag
     3121 aaagattatt tgttttatgc tgctagttgc tgttgttgca ggctgtggtg ccaatgtacc
     3181 aacatgggat gaaatcaatg ccatggtgga tggtggagaa tacaaatcaa agattgatga
     3241 ggccataaaa caagccggcc cagaaaacca ccctgcatca aaggtcgtga tttcgtattt
     3301 tgaaaacctt ttttctggca aatatgacga ggcctggtca cagatagaaa agaactcgcc
     3361 atttcagact gcaagggtca gcaaggaaca gatggtcgag atgtttgaaa aagccaaggc
     3421 cgaggtggac tacaaaagcg caagggtcat ctccctctcg atcatgaaaa aaaatgccaa
     3481 tgtgagaatg gtcaaagttg gtttcatgct atacgtttat gacaagctca aaaaacagga
     3541 tgacacaccc attggctctt atacactcag cgatcttact ggcgagtgga agatatttga
     3601 tagcgagata aagtaggaag tttcttattt tataaatctc ataaataaaa agcccctggg
     3661 aaaaccagag gctttagaat taaaaacaga tttttcttgt tagatcaagc tgttttgtcc
     3721 attgcaccga tgagaacggc gattattcca attgcgattt tcaaaattgc atgccacatt
     3781 ggctcaacac caagcccgaa ccatgaccat atccccgaaa ggcccataat caccagcaaa
     3841 ataccaacta taatcaacgt aatttttgac atttaaccta aattcctcct ttcgtaagac
     3901 ctattataaa caacccagaa agtttgtcaa atattaggct tattgcaaaa aaatgatatt
     3961 aattagatta acatttaatt gttaatttaa caaattggtc tcttcgcaaa taattcctcc
     4021 cttggagagc tggagcatta tggaggcccc cttcttgaaa ctaatgtcaa tggtcttttt
     4081 ttggcccaaa aatgctgcat cgagctccat tttgacctct gtggttccat ctgacaaccc
     4141 ataaggcatc gggtatgaga ttgcccttgt cgctggattt gccatgaaaa cgtttatccc
     4201 gtcacctgtt tttctgcacc agtattcggg ctttagctta tttgttgcgg tgaaaagtgg
     4261 cagtaatgtg gacgattttt tgatgttatc tgatacgttt tcaaatgagg ccagcttcgt
     4321 tattgccttt tcgtaagcag gagaagcaat aaaacctggt tgttccggaa tttgtttcag
     4381 gcatatcctc gcacccttct gtgccaggct gataagtgca tcaagctctt caaagtccat
     4441 atactggcag tcgacataga tgaaatcaaa ctccaggttg ccaatgacgg taaagccgtc
     4501 cctgacctct gctcttttca agaatggtgc tgttatccag cacgggcgat ggccccagag
     4561 ttcttccgac atcctctggt agtggaattc ccagtggtat tttgcagacg gcctcttcag
     4621 tttgtcttca agctcgcctt ccatccatgc gtcctcgacc ggaagcatga cggcacactg
     4681 gaaatatggt ttgccctcga gcagaaaatc ggagattttt ttcatatagc catttaatgc
     4741 tggcaagtct tctgcaaagc ttgcgtctgg gccaacgtgg acgcttgcat agaaagaatt
     4801 tttgccaccc tccggattgt atggcatgcc gtgccagatg accttgttgg tgccgtttgc
     4861 cagcatggcg tctgcaagca gcttcaggtc gccggttttt tcttccctct ggtgctgacc
     4921 cgggcctggc caccccttcc atccgtagag gcaggtaaag gtttcggccg ttacatattt
     4981 tttgccagcc agcgcggcag agcttgcggc aagctgtgag aaatgcggat caaagaggac
     5041 tgcctcactc tctggaacgt cacatgttgc gtatccctcg agtatgtcga ctggtgcccc
     5101 atggacctgc actcttgaga aagaattatg ttccctgcac catctggcaa atggactgaa
     5161 aaaattttcc aggagcattt ctgatacgag tttcctgtag tcgtatcttg cccccgggct
     5221 ggaatccaga tcatccatga atgggactat gtcatatccg aaagcagact tgaagtcatt
     5281 ttcaaaacct tttgcccaga ggtcttcggt agcgacctcg aatgagtcgc agaacaggca
     5341 ttgttttttg ccatcaagcg gcaggaagtt gcccatcttt tctgcgtagc gaaggaacgc
     5401 gccctcgttc atgtggtcaa gaacaaacgt ttcaggataa gttcttgcct cccatgattt
     5461 tcttatccgc tgtttcgatg tgccatagaa atcttttgag gcatcctcct cactcaccat
     5521 cgacccgcca aagggccaga gggtgccaaa ggtgaaatca catgcgagcc ccaatgatct
     5581 ggccctttct tttgtatggc ggattttatc ggagagttct cttgaaagcc acgcagggcc
     5641 ttccggactc tccggaagcg gatagaccca tgctatctcc actccaccga agccattgtt
     5701 ctttagccac gagagctgga aatcaatgtc tttctcgttg atggggcctg aaaaccacca
     5761 ccatctggtc aggggcttgc tatctgagta tggttccatg ggccagatgg tagcctttta
     5821 ggggactgcc ctcaagcccc ctgtgctatt tgcgatcctg agcttgagcg aacctagtgc
     5881 aatggaggta gaaatggaca acgctggcga aggattggca agatacgcct ggtttaccag
     5941 ccggccatct tttgtggtga aaccactgcc catttctact ccaccaagcg ttgtttcact
     6001 tttcactgca accgggacat ctgacggaac atcaaggtcg agcgatgcaa cacctatctg
     6061 gatgtttgca ttgcaatcct gaccaagctt gccggtgaag tcaatcttgt acgaggcggc
     6121 cccgccctcc accttgagat ttttaaatcc tgcattcaag agttttgaca gtgtcatcga
     6181 accggccccg gtagagaccc tgaatgaatc cattaggatg ggattgggtt tcaggaatga
     6241 catctcgaat ttgcctgcgc caaaggagac ggcaactgac gagagcggca gtccgcccag
     6301 ctcgatgcag ttctcgcttg cccctccctc tatcacaaga tcgaaaggat tttgtgagga
     6361 aagcacaagg tcaaatgttg gcgtcctgtt gaagacattt ataagtcccg agatgtttct
     6421 tgattgcgag acgctcactt cattgccaga ttgttctata atgcacggca ggttgtcagt
     6481 agggtcatca taggtccctt ccacccacga tgtgccatcg cctggtctca gattgaactt
     6541 gcaagcgcct attgaaaact tgagttttgg cttctcgcta gctgggaatg gtattgaaat
     6601 tggggttttc attgctcctc ctcgaccctc tcgacaacaa ttgtcatttt tgtaaccagt
     6661 gcggctattg ctccaattgc tgcccatact ggcaacaaca ctgcaccaat gacgccaagc
     6721 gtcaatggta tttcaatcag cgttgaacca tggtcatttt ttatgatgat tcttctaatg
     6781 ttgccctcat ggacgagttc cttgattttt tcgatgactt tttcacctga aacctggaat
     6841 tcttctgttc ggcatttttc tggcatgttg tttctccttt ccctttgcaa aaatttgaca
     6901 ccttattatt gtattagttt agggatttac aaaaaattga aacttggagg attaaatgaa
     6961 aaaaattctt gctttagctt gtgtcgctat tctttttgcg ggttgtggtg gtgcagctgc
     7021 tggcccaacc aaagttaccg aggaagacaa gggaaaaacc tttgaggcaa cagttggtca
     7081 gaagtttgag gtaacactca aggccaacga aacaacgcca gttcagtgga ccggcccgaa
     7141 gatcgagggc gacgcaataa ttggaaccgt gaattctgaa tacaaaaccg aagaaaatcc
     7201 agaaggaatg gttggagttg gcggctggag gatatacacg tttgaagtca cagcggctgg
     7261 aacagcaaag atggtttggg aatccaagca tattgctgaa gaagggccac cttacgatac
     7321 tttcgaaatt acaatcaacg caaagtaatc tggtacttgc atatttaggt taaatccttt
     7381 atctttttta acaaaagatg agggataaat tagtaattat catccttgtc gtgatggggc
     7441 ttgctggaca atttgctcca gcatgcctct ccggctcaaa tgagatacgc cttgaaggcg
     7501 tgctttacac gaaaaataca gagaagaatt tgtttattct caaaagtggc gactgccttt
     7561 ataaattttc cccaaagaat gccaatgaaa tggaaaggtt gcgttccgga gattccctgg
     7621 tccttaccgc cattcaggat gccaatagaa aatttgtcct tcatgatgca acgttcaaaa
     7681 ttgtctatga caagacagta acaactgttt tgtgggcaaa actttccagt ggcggatggc
     7741 atgatggcct cttcagggtg ctaacatcta atgatgaata ctggcaggtc aatttcatat
     7801 cgggtgaggg gacagccggg ctaaaatctg aaacatatta tactttcagg ggtgcacagg
     7861 accatcacct ctcacaaaca atcaataatg catctgttta tgacactgtg aggcgtaaaa
     7921 tatcccttct gaaagttgtt tcggtagatg ccaaaacagg gtggcttgaa accaagaata
     7981 cctctgacaa tgacatccgg gtttacagta ctgacgcagc tgaaaacctc gccaacatga
     8041 aaaaagatga ttttataaga gtcgagggct atcaagacgt aaaagacagt tatggcccga
     8101 tactgtttgc atccatatgc acgaaaaatc cttgcccaga aactgtcagc gttgaatttg
     8161 tcggggaagt gcttgatgtg ccatcaaaga ttaatggaaa caaatatagc atcagggagt
     8221 gcgattcagt catcaatttt gaatatgatg gtccaaatgg taaatcttca attgggaact
     8281 gggtcaaggt caagggaagt tactctctga aggaaagact ttgtattcca agttcgataa
     8341 ctttgaccaa cccggcaata atctgtacgg tttttgaaag agacccagta agccgtgatc
     8401 tccgtggcat tgaaccagaa gtcaatatta gatggaatat ccagctttct gagaagtcga
     8461 gatcaaagag tgtgccattg ggaacaatgc taaaggtttc atcttcatcg tatttgcaaa
     8521 gaaggcttat tggctgcaaa acgtcagatg ttgtcaaaat ctctggcatg ataatagaca
     8581 tcgactacga acttcagact gcaactattg agagtgttgc aggagagaaa acaacaaccc
     8641 acatacgacc agaagtggtt gatttgctat cgttcaatta tgaagatcaa gttgaggttt
     8701 atggtttgca tgcgaccaaa tcttcacaag aaggtcatat ctttgagtgt gtcctaaaaa
     8761 aggcaagcaa gtaaacactt tttcaaatgc aaccgtaacc ttgttgtcag aaaaaatatc
     8821 aaatagatga taatggtcct ggatttatct gtttaataat tttgtttaag gagagaaaaa
     8881 atgtccaccg agattattga agtttcacag aaaagagaat tgaaggagtt tattcatgca
     8941 cccacaaaat tgcacagggg gcatgaaggt tgggcaacac cgctgtactc agacgagtgg
     9001 aagttttttt cgaggaggaa aaacaaaaac ttcaaccaca gcgacacagt gatggttctg
     9061 gcaaaaaaac gcgggagaat tgttggcagg tgcatgggaa tcataaacaa acggttcctt
     9121 gagatatccg gtgaaaaaac ggccaggttt ggctatcttg aatccacaga cgacattgag
     9181 gtgacaaaag cgttggtaac acatgtggaa aaatgggcca aatccagaaa catgaacaaa
     9241 attgttggac caatgggatt tactgatcaa gaccccgagg gtttcctcat cgagggtttt
     9301 gggaatgaac caacattggc gacttattac aactacgagt ttataccaga acacattgag
     9361 aaacttggat atggcaaaga ggttgactac gtagtctata aaatcccagt tacaagcgag
     9421 tatccgccaa tttacaaaaa ggtgctggaa agattgtcca agaaaaatga attcaaactt
     9481 gtggaattta gctccacctc tcaaatcaag ccttacattg tgcctgtgct ctcactggta
     9541 aacgatgtat tttcgcacct ttatggctac gtcccattgg cgatggaaga aatggaagac
     9601 cttgcaaagc aatatctgcc agccctaaac ccaaaattcc tcaaggttgt tgaaaaagac
     9661 agcaaacttg ttggttttat ccttggaatc cccaatatgg acgagggttt caggaaggca
     9721 agaggaaggc tgcttccatt tggtttctac cacattctct ccgcatcaaa aaagtccagg
     9781 cagcttgatc tcctgcttgg tggaattgac agggagtaca gggatagggg tattgatgta
     9841 cttcttggct cgtcactgat gaaatctgcg catgattctg gcatcaaatt catggacagc
     9901 catcttgagc ttgaatcaaa cacgctggtc caggcagaga tgaagaggtt tggcgggcag
     9961 gtgtacaaaa aatacaggat attccagaaa aaactctagt caaattttga aaaaatgcta
    10021 cttgtgatat aattcctgca aacgattgac gggagtttac aatgggaaga gctttgctgg
    10081 ttgtgattgt gcttgtgtca caattgttgt tgcctgttcg agggcttaag gcagagccat
    10141 gcttttattc gcatgctggt tcaactgggt gcggtcaggc aagaatttct ggtgaagaag
    10201 acaaggtaaa ggaactcgat cttgagggtt gcccgggtga caaaaatgac ctttgggtcg
    10261 acctgactgt cacatcgggc ggcactgcaa gtgttgaggt tttatggttg ccgccagaca
    10321 atgtttcacc cagggactgg aacggttcat ggatggaccc gaccccaaag caattccagc
    10381 ttgaagaaga cgcaacaatc aagattgcgc ttaacatcaa gtggcacatg ctcaaagtcg
    10441 gtgagatgtc aaaaggtagg ctggtttttg ccatgacctg ggtgattgcc ggcaaaaaag
    10501 tcacaaaaaa cctgatttac aaaaccacca tgacaatccc tggcccaaaa ataaaagccg
    10561 aagatgttgt tgtaaaatcg gtttgttctg atggcgctat atcaaagttt ggtattccag
    10621 ttgagaattt tggttgcggg atgaagggcc agatagtgtt tggcgagtgt aaatttcttg
    10681 agaaaagcca tatatttcca gtggatattg cccagaggga agagggcaag gggaagtctg
    10741 ttactatggt cgcaatcgac ggccttgcag taagaatgag ggaccgcaag gtcatgctcc
    10801 cagtttttat ccgtgatctt tcgagaacac ctgttattga tgttccgtta tgcagcgtta
    10861 cgcttctttc gaacatgcca cctttgcttg aaggtagcca gaccttttct gtggtcaggg
    10921 gaacgaagac agcttttggg gttgcatgca gtgacgagga aaatgatccg gtcgagttct
    10981 ctgttcttgg tgcaccgcca tggtgcacca ttgaaaagaa gggca
//
LOCUS       DAOSRI010000021        10878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5923_length_10878_cov_3.890234, whole genome shotgun sequence.
ACCESSION   DAOSRI010000021 DAOSRI010000000
VERSION     DAOSRI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_10878_cov_3.890234"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..623)
                     /locus_tag="PK717_01205"
     CDS             complement(90..623)
                     /locus_tag="PK717_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOR46321.1"
                     /translation="MKTRDLVLVAIFVSLSVISAFLKIPLPGLPLTFQLATAIASGIF
                     LGKVLGPASQGIYLVLGLIGLPVFAKGGGPMYVLQPSFGYLLALPLSSFLTGWLWDKQ
                     GWKKIISVAAGVIVAFAIGIAYTCICTYFFLGNQKAIADILSITLLVLFAKDIVLGYI
                     IYIIGSVLSPYYGRNRQ"
     gene            complement(671..1774)
                     /gene="hemW"
                     /locus_tag="PK717_01210"
     CDS             complement(671..1774)
                     /gene="hemW"
                     /locus_tag="PK717_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOR46322.1"
                     /translation="MIGLYVHVPFCAKKCDYCDFYSLAKSEGRIHGYVEAVLTEAKQY
                     QGLYFDTIYFGGGTPSLLGPENLEKLYSGLRRTFGFEKILEATIEANPESLTEEFALS
                     AINCEFDRVSIGIQSLNDGELKSVGRIHNSKKAIDAIAIANRYFKRVSADLIIGLPGQ
                     TKSSLLSTLSKFVEFGLKHISAYCLSLEEGTKIYQNMPGNLPSDDDQAILFEECRDLL
                     VSKNFHHYEISNFAISEEESLHNINYWKCSEYLGLGPGASSHLDGIRFKNEENLEKYI
                     SNPLSCKFIEEKLSSEDKIFEEAMLNLRLLENGTDLSLLAQRYDPESISKLQSRLDEL
                     VQDGKIDKNGSIYTINPSFILVSNPIFSKILSG"
     gene            complement(1771..3567)
                     /gene="lepA"
                     /locus_tag="PK717_01215"
     CDS             complement(1771..3567)
                     /gene="lepA"
                     /locus_tag="PK717_01215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOR46323.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRILELTDVVHTTGNAQILDSMD
                     LEKERGITIKASPVRIPYKAKNGQEYILNLIDTPGHVDFNYEVSRSLKACEGALLLVD
                     ATQGVEAQTIANAYLAMDADLHIIPVINKIDLPNAMIDECKIQMEDMLGLPSDDAMLV
                     SAKDGTGVPELLEEIVRKIPPPRISESAPLRCLIFDSHYDSYKGAISHIRIFDGSIKP
                     GDKIMLKSNSKIFEVEEVGYFGLRLIRCDELKAGEVGYFAAIIRDTNDIHVGDTIISP
                     EFPDTMAIPGFRRMQPMVFCGVYPISTNDYDKLRTAIEKYQLNDSSIVYEKESSAALG
                     HGFRVGFLGMLHMEIVVERLKREFEQEIIATMPTVVYKVIGTKGQETYIDNPSKFPDP
                     NEIESIEEPIVKAQVITQNDTIGPVMELSQARRGTMTGMEYPDQKRTIIHYEFPLAEI
                     ITDYYDKLKSITHGYGSLDYDFTGYRPDDLVKVDVLVNGDQVDALSYISHRDFAVQKG
                     RSLINKLRKLIPRQQFKIPLQAAIGGKIIAREDIVAYRKDVLAKCYGGDVTRKKKLLE
                     KQKEGKKRMKMVGAVEIPQEAFLSLLKVEEEE"
     gene            3667..6321
                     /locus_tag="PK717_01220"
     CDS             3667..6321
                     /locus_tag="PK717_01220"
                     /inference="COORDINATES: protein motif:HMM:NF005829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOR46324.1"
                     /translation="MEKTLILTEKPSVAADIAHALGGFDKKEDFYESDDTYITWAVGH
                     LVTLAEPEDYDKGYKYWTLQSLPIIPNKFELKPIPKSESRLKKIASLAKKSSTLVNAC
                     DAGREGELIFRYIYEYLGLEAPTKRLWLQSMTKSAIADGFERLRPSKELNNLGESAKC
                     RSEADWLIGINATRAFTRRLGNLLSIGRVQTPTLSIIISREKEINAFVPRTYFDIFAE
                     FEAKSGVYRGKWFIDVPKVSKEKNAPITDDILASLVKKYGQIATSALKANDGAVQGDT
                     VESVGDKKAADEIVNRIKGKEGKVIVEDHKLQKQSPPLLFDLNELQREANRRFSFSAA
                     RTLQLAQQLYEEKKLVTYPRSDSKHLPEDYTDVVPRIFGILSHTKYDKVALPLVGQKV
                     AKNKRIFDNKKISDHFAIIPTEKSLEEVSLTPEQTKIYDLIVKKFLAAFYPDAVWEVV
                     HRVTQVDGLDFKSDAKSLKEKGFYAVYGREEQEESSLPVVAEGETVETVAQELQEKQT
                     TPPPRYNEATLLGAMETAGKFIDDEELRDAMKEKGLGTPATRASIIERILEVGYIERV
                     GRDLVPTTKGIALYDLLIGFPLPELYSPEMTGNWEYKLACIEKGEMSPEDFLNEIVDF
                     TKQLVNKVKDRSLDAPIASQDGNGEARAIGTCPLCGEDVVDSFMSFRCANYKSTKTKK
                     GAKKTKAKEEDEESGCNFAIWKTIAGKIITEDIAKELIEKKKAGPLSGFRSKSGRPFS
                     ATLLLGDDGKITFEFSKSQDEGGQEKTKIEEVFLEDPVAKCPVCKADILEGTSVYRCQ
                     NFGKSCKMSLSKVILGKAIARETAARLFEEGKTEKISGFISRKGRAFSAYLVLKDDGK
                     VGFEFDNATGRTAKGASKVSKPKTKKSK"
     gene            6368..8809
                     /locus_tag="PK717_01225"
     CDS             6368..8809
                     /locus_tag="PK717_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOR46325.1"
                     /translation="MAIALALFIYRVGSDVVATLPNLDEQKRPPVASQIFDYDNQLIG
                     SVFFEDRIPVKISEVSKDFLNTIVTSEDERFYQHKGFDLRSIFRAFFVNIKTGSMSQG
                     GSTLTQQVARSELFIDSKETSVTRKIKEIILAFKLEQRYTKEEILEAYVNQIFFGFGS
                     YGVESAARNYFGKHAKDLNYAESSLLVAILPAPSVDNPIINLAGTKERQKNVLRKLVV
                     NNVITEEESKKYFEVPYQRKSFREVGDNDSVNYFIDYVKAEMEKRYDPKDLKEGGLRI
                     KTTLIRSYQKYAENAMRNVFAKAEKSGEFSSKVFDEFGVKQPQACFVAMNPRNGYVLA
                     MVGGRDYKNTKWNRTTAERHPGSSFKIFDYSAALENKSVTPASILKSDYSFNVNGWNP
                     GEWNYSVTNKLYTVRGALLTSSNVCALRAGLRVGLDRVAYYAAKMGLKTPIMPYPSIT
                     VGSVEVRPVDMCTAYASLASGGIRHDPVAILEVQNRDGIIIERYQGTGFRVLSEETAW
                     ILGNIFQDVLHSILGSTIKTPAAGKTGTSTGSLNAWYCCFTPEITVTCYVGKDSKKVM
                     SAVNTIIWGSSMAAPMARDFVNACLTDKKHPLPKTPFPPAPKGMTGSIFCKTSGELVN
                     GFCPRDQISSDYFFAGTTPDTPCQYHKQGVFPYNVVEEIVDGKKYYYKPGNSWCVTKE
                     VLLSKDVYEKLQTKSDCPALINLTISDQSGQLMQEPYSLSKGNIYNLDVSIPQDVLGK
                     FNEIDVYFGDMRVKKWQEGDLDSDTGQKVEFSSPLNAMFAVSPDDADKTKKLIFEIRG
                     PGGFYLKKTFDVGIS"
     gene            8817..>10878
                     /locus_tag="PK717_01230"
     CDS             8817..>10878
                     /locus_tag="PK717_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOR46326.1"
                     /translation="MLPVPLPRTKTKKPRGFPFLGFFVLIILVVVFSAAFYLAFIVSD
                     YDVRWLPTILDPQNHKPPNPHETVNETNRIFDAEGNLIHEFHGDEDRIYVPLEQISEW
                     ARKAIIASEDVRFYYHKGFDMRATLRAFFKNVQTREASEGGSTITQQLARNMYLGTGK
                     TLSNERTFTRKLKELLLARKIEQAYSKNEILEFYLNQVYFGSRAYGIESAAKRYFGIP
                     AKNLTLAQSSMLAGVLSSPSFNNPYDNLAEAKKAQEVVLEKMAKNGFITDVQMEKASK
                     EKLNFRPYSIQLKAGDSLGYFIDYVKDELYDKLGEFPIKKGGLDIYTTINQDCQKYAV
                     QAVNKVLGEAIKAGDFGKIQKDKLGVEQPQVALCAIDADTGQIQAMVGGRDYKNTQYN
                     RCTALRQPGSSFKILVYSAALQHGVLSPSSLVRSSPISIGDWQPKEWFRGYFGTVTVK
                     YALQESSNICAIRAALKVGLGNVVATAKKMGISTSKLLAVPSIAIGYVEVKPIEMASV
                     GQTISNYGTHIPPIAIKRIENRKTGKTIDPAYGQPTRAIDEPTAYDMISMLKNVVERG
                     TGMSAKVPGVPCAGKTGTTGSFRDGWFVGFTPEISASVYLGSDSREVDLSFVSNYGSK
                     YSARIWKEFISMYSKGRTIKDWVDKNPDDYVRVRVCEETGLIANDTCPGYYMLVRKSK
                     MPSER"
BASE COUNT         3127 a         2423 c         2495 g         2833 t
ORIGIN      
        1 tgcaacgata aatgattaca attacctttt gtcaactaca gggcaaaaat tgtacaccct
       61 attcctgccc tttcaagata ctcttgggct tattgcctgt tccttccata gtatggagag
      121 agcacagatc cgatgatgta tataatataa ccgaggacaa tatcttttgc aaaaagcaca
      181 agcagagtga tactcagtat atctgcgatg gctttttgat tgccaaggaa aaaataggtg
      241 cagatgcatg tgtatgcaat tccaattgca aaagcaacga tgactcctgc tgccacggaa
      301 attatcttct tccagccctg cttgtcccat agccaccctg taaggaacga ggagagcggg
      361 agtgccagaa ggtagccaaa agatggttgc agtacataca ttggcccgcc accttttgca
      421 aagaccggaa ggcctatcag acccagaaca agatatatgc cttgggatgc cgggccaagc
      481 accttaccaa gaaaaatacc ggatgcaatt gctgttgcaa gctggaatgt gagtggcaat
      541 cccggaagcg ggatctttag gaaggccgaa atgacagaaa ggctgacaaa aatagcgaca
      601 aggaccaagt ctctggtttt catgctggaa atactattat tgttttgatt tttggcaacc
      661 aacaatccag tcatccgctc aggatttttg aaaaaatagg attggaaacc aggataaatg
      721 agggatttat tgtgtaaata ctgccatttt tatcaatttt gccatcctgg acaagttcat
      781 ccagcctgct ttgaagtttg gaaatacttt ctggatcgta tctttgtgcc agcaaactca
      841 gatccgttcc attttccagg agcctgaggt ttagcattgc ctcttcgaat attttgtctt
      901 cagatgaaag tttctcttcg ataaatttgc atgaaagcgg attgcttatg tatttttcga
      961 gattttcctc gttcttgaat ctgataccgt ctaggtgtga tgatgcaccc gggccaaggc
     1021 caagatactc actacatttc cagtaattga tgttgtggag tgattcttcc tcagaaatgg
     1081 caaaatttga aatctcgtag tggtgaaaat ttttggaaac cagcaaatcc ctacactcct
     1141 caaaaagaat tgcttgatca tcatctgatg gtaagttgcc aggcatgttt tgataaattt
     1201 ttgttccttc ctcaagcgaa aggcagtagg cggagatgtg cttaagccca aactctacaa
     1261 acttggacaa tgtcgacagc aaggagcttt ttgtttgtcc aggcaatcca attattaaat
     1321 cggccgagac tcttttaaaa tatctgtttg cgatggcaat agcatcgatt gccttttttg
     1381 aattgtgtat tcttccaacc gacttgagtt caccatcatt taacgattgg ataccaattg
     1441 aaaccctgtc aaattcacaa tttatggccg agagcgcaaa ttcctccgtc aggctttccg
     1501 gattggcttc tattgtggct tcaaggattt tttcaaatcc gaatgttctc cttagccctg
     1561 agtatagttt ttcgagattt tccgggccaa gtagacttgg ggtaccgcca ccaaagtaaa
     1621 tagtgtcgaa atacagcccc tgatattgct ttgcttctgt aaggacagct tcgacataac
     1681 catggattcg tccttcagat tttgcaaggc tgtaaaaatc gcaatagtcg catttctttg
     1741 cgcaaaaagg aacgtggacg taaagcccga tcactcttct tcttcaacct ttagcaggga
     1801 caggaaggcc tcctgcggaa tttcgactgc gccgaccatc ttcatgcgct ttttgccttc
     1861 cttctgtttc tcgagcagct ttttctttct tgtcacatcg ccaccgtagc attttgcaag
     1921 aacgtctttc ctgtaggcca ctatgtcttc ccttgcgatg atctttccgc ctattgcggc
     1981 ctggagcggg attttgaact gttgccttgg gatgagcttc ctgagcttgt tgatcagcga
     2041 cctccccttt tgaacggcaa agtccctgtg ggagatgtat gagagcgcat ctacctggtc
     2101 accattgacc aacacgtcaa ccttgacgag gtcatccggg cgatatcctg tgaaatcata
     2161 atcaagactg ccatatccat gtgtaatact tttcagtttg tcgtaatagt ccgttattat
     2221 ttcggcgaga gggaattcgt agtgaatgat ggttctcttc tgatctggat attccatgcc
     2281 ggtcatggtg cctctccttg cttgtgagag ctccattacc ggaccaatcg tgtcattttg
     2341 tgtgattacc tgggccttta caattggctc ctcgattgat tcaatttcat ttggatcagg
     2401 aaacttggag gggttgtcga tgtaggtttc ctggcctttt gtgccgatga ctttgtaaac
     2461 aacggtgggc atcgttgcga ttatttcctg ctcaaactcc ctttttagcc tctcaacaac
     2521 aatttccatg tgtagcatcc caaggaagcc aaccctgaaa ccgtgcccaa gtgctgcaga
     2581 actctctttt tcgtagacaa tcgagctgtc gtttagctga tacttttcaa tggcggtgcg
     2641 aagcttgtca tagtcattgg tcgagatagg gtatacacca cagaagacca ttggctgcat
     2701 tcttctaaaa ccagggatgg ccatagtgtc tggaaactcc ggagaaatta ttgtgtctcc
     2761 gacatgtatg tcgtttgtat ctcttatgat tgcggcaaaa tatccaactt caccagcctt
     2821 cagctcgtcg caccgaataa gtctcaatcc aaaatagcca acctcttcaa cctcaaagat
     2881 tttgctgttg gacttgagca ttatcttgtc acccggtttt attgaaccat caaaaatcct
     2941 tatgtggcta atggcaccct tgtaggaatc atagtggctg tcgaatatca ggcacctgag
     3001 tggtgctgat tcgcttattc ttggtggtgg tatcttgcgg acaatttctt caagcagttc
     3061 tggcactccg gtgccatctt ttgcagagac gagcatcgca tcgtcagaag gcaggccaag
     3121 catgtcttcc atctgtatct tgcattcatc gatcatcgca tttgggagat cgattttgtt
     3181 tatgaccggg attatgtgga ggtcagcatc cattgccaga tatgcgttgg caattgtctg
     3241 agcctcaacc ccctgcgtgg cgtcaacgag gagaagcgct ccctcacagg ccttcaggct
     3301 ccttgaaact tcatagttga aatccacatg gcctggggtg tctatcaggt tcaggatgta
     3361 ctcctggccg ttttttgcct tgtacgggat tctgactgga gaggccttga ttgttattcc
     3421 cctctccttt tccaggtcca tgctgtccaa aatctgggca ttgcctgttg tgtggacaac
     3481 atcggtcagc tccagtattc tgtcggccag cgtcgatttg ccatggtcga tatgggctat
     3541 tatgcaaaaa ttgcgaatat tgttcatacc aagtaatcat tttactgcca gtcgcaattt
     3601 gttcaaggtt tgacactcat ttccttgaca gccatgaagc ccataactat acacggcacg
     3661 taaactatgg aaaaaacatt gatattgacc gagaagcctt cagttgccgc cgatatagca
     3721 cacgcactag gcggtttcga caaaaaagag gatttttacg agagtgacga cacctatata
     3781 acatgggcag tcggtcacct tgtaacactg gctgagcctg aagattatga caagggttat
     3841 aagtattgga ctttgcagtc ccttccaata atccccaata agttcgagct caagccaatc
     3901 ccaaaatctg aaagcaggtt gaagaagata gcttcgctgg caaaaaaatc atcaacgctt
     3961 gtaaatgcct gtgatgctgg ccgcgagggt gaactgatat tcaggtacat ctatgagtac
     4021 cttggacttg aagcaccgac aaaaaggttg tggctccagt ccatgaccaa atcggccatt
     4081 gctgatggtt ttgaaaggct tagaccatca aaagagctga acaaccttgg ggagtcggca
     4141 aagtgcagat ctgaggccga ttggctcatt ggaatcaatg caaccagggc ctttacaagg
     4201 agacttggca acctcctctc gattggcaga gtacagacac caacactctc gataatcatc
     4261 tcgagggaaa aagagataaa tgcctttgtc ccaaggacat attttgatat ttttgcagag
     4321 tttgaagcca aatctggtgt ctacaggggg aaatggttta tcgatgttcc caaggtgtca
     4381 aaagaaaaaa atgcaccaat aaccgatgat attttggcct cactagtaaa gaaatatggc
     4441 cagatagcta catctgccct taaggccaat gatggtgccg tccagggaga cactgttgaa
     4501 tctgttgggg acaagaaagc tgctgatgag atagttaaca ggatcaaggg aaaggaaggc
     4561 aaagttattg ttgaggacca taagctccag aagcagagcc cgccgctcct ttttgatctc
     4621 aatgaactcc agagagaggc caatagaagg ttttcatttt ctgctgccag aaccttgcag
     4681 cttgcccagc aactttacga agagaaaaaa ctggtcacct acccaaggtc tgacagcaaa
     4741 catcttcccg aggactacac ggatgttgtt ccgagaattt ttggcatact ttcccacaca
     4801 aaatatgaca aggtggccct gccacttgtt ggccagaagg ttgcaaaaaa caagagaata
     4861 tttgacaaca agaagatctc tgaccacttt gcaataatcc ccacagaaaa atcgcttgag
     4921 gaagtatctc tcactccaga acagacaaaa atttatgatt tgatagtgaa aaaattcctt
     4981 gcagcgttct acccggatgc agtctgggag gtcgtgcaca gggtgaccca ggttgacggg
     5041 cttgatttca agtccgatgc caagagtttg aaagaaaaag gtttctacgc tgtctatggc
     5101 agggaagagc aggaagaatc ttctctacca gtggttgcag aaggcgaaac agtggaaact
     5161 gttgcacagg aactacagga aaaacaaaca acgccaccgc caagatataa cgaggcaacc
     5221 ctccttggag caatggaaac ggctggcaag tttatcgatg atgaggaact tagagatgca
     5281 atgaaggaaa aaggcctggg aactccggcc acaagagctt ctatcatcga aaggatactg
     5341 gaagttggct acattgaacg tgttggcaga gaccttgtcc caacaacaaa agggatagcg
     5401 ctgtacgatc ttttgattgg tttccctctt ccggaactat attcaccaga aatgaccgga
     5461 aactgggaat acaagcttgc ctgtattgaa aaaggcgaga tgagccccga ggatttcctt
     5521 aatgaaattg tggatttcac aaaacagctt gtaaacaagg taaaggacag gagtcttgat
     5581 gctccaattg ccagccagga tggcaatgga gaggcgaggg ccattggaac ttgccctctc
     5641 tgcggggaag atgttgttga ctcttttatg tctttcaggt gtgccaacta caaatccaca
     5701 aagaccaaaa aaggcgcaaa gaaaacgaaa gccaaggaag aagatgaaga gagcgggtgc
     5761 aattttgcga tctggaaaac gattgccgga aaaatcatta ccgaggacat cgcaaaagag
     5821 ctcatcgaaa agaagaaggc aggtcctctt tccggtttca gatcaaaatc tggcaggcca
     5881 ttttctgcta cccttttgct tggcgatgac ggtaaaataa ccttcgagtt ctccaagagc
     5941 caggatgaag gtggccagga aaaaacaaaa atagaagaag tcttccttga agaccctgtt
     6001 gcaaagtgcc cggtctgcaa ggccgacatc ctcgagggaa cctcggtgta ccgctgccaa
     6061 aactttggca agtcttgcaa aatgtccctt agtaaagtaa ttttagggaa ggccatagct
     6121 cgcgaaacgg ccgccagact ttttgaagaa ggcaaaacag aaaagatttc aggcttcata
     6181 agtcgcaaag gcagagcgtt ttctgcgtat ctggttttaa aagatgacgg gaaggtaggg
     6241 tttgaatttg acaatgcaac aggaaggaca gcaaaaggcg catccaaagt ttcaaagcca
     6301 aaaactaaaa aaagcaaata ggcttttctg gacagtttta aggttcggcc ttgccgtcgg
     6361 ttttgtagtg gccattgccc ttgctctttt catatacagg gtcggatcag atgttgttgc
     6421 gactttgcca aatcttgatg aacagaagag gccgccagtg gcgtcccaga tatttgatta
     6481 cgacaaccaa ctaataggct ccgtcttttt tgaggacagg atacctgtaa agataagtga
     6541 agtttcaaag gatttcctca acacaatagt gacctctgag gatgaaaggt tttaccagca
     6601 taagggtttt gatcttcgtt caatcttcag ggcatttttt gtcaacataa agacaggttc
     6661 catgagtcag ggtggttcta cgctgaccca acaggttgcc cgttcggagc tttttataga
     6721 cagcaaagaa acttcggtca caaggaagat caaggaaata atactggcat tcaagcttga
     6781 gcagcgttac acaaaagaag agattctaga agcctatgtc aaccagatat tctttgggtt
     6841 tggctcctat ggtgtggaat ctgcagctag aaactatttt ggaaaacatg caaaagacct
     6901 taactatgca gaaagctccc ttctcgtggc gatccttcct gccccttcgg tcgacaatcc
     6961 gataatcaac cttgctggta caaaagagcg ccagaaaaac gttttgcgga aacttgttgt
     7021 aaataatgta ataactgaag aagaatcgaa gaaatatttt gaggtgccgt accaaagaaa
     7081 atctttcagg gaagtcgggg acaacgattc tgtaaactac ttcatcgact acgtcaaagc
     7141 cgagatggag aagcgctacg atccaaagga cctgaaagag ggtggcctca ggatcaagac
     7201 aacgcttatc aggtcctacc aaaaatatgc cgagaacgcc atgagaaatg tgtttgcaaa
     7261 agctgaaaaa agtggcgaat tctcctcaaa agtttttgat gaatttggtg taaaacagcc
     7321 acaggcttgt tttgtggcaa tgaacccccg caatgggtat gttctcgcga tggttggtgg
     7381 aagagactac aaaaacacaa agtggaaccg cacaacagca gaaagacacc ccggctcgtc
     7441 attcaagatt tttgactact cggccgcact tgagaacaaa tctgttaccc ctgcttccat
     7501 cctcaagtca gactacagct tcaacgtgaa tggatggaac ccgggtgaat ggaattacag
     7561 cgttaccaac aagctttata ctgtaagagg cgcacttttg acctcttcga acgtctgtgc
     7621 attgagagcg ggccttaggg ttggattaga tagagtcgct tattatgcag ccaaaatggg
     7681 cctcaagacc ccaataatgc cttacccttc aatcaccgtt ggttcagttg aagtcagacc
     7741 tgttgatatg tgtactgcct atgcctccct cgcttcaggt ggaattcgtc atgatccggt
     7801 tgccatactg gaagtacaaa acagggacgg gataataatc gagcgctatc agggcacagg
     7861 ttttagagtc ctctccgaag aaacagcgtg gatacttgga aatatttttc aagatgtttt
     7921 acactctatt cttggctcca caataaaaac accggcggct ggaaaaactg gtacttctac
     7981 tggttcactc aatgcctggt attgttgctt tacaccagaa ataactgtta cctgctatgt
     8041 tggaaaagac tcgaaaaaag tcatgagtgc tgtcaacaca atcatctggg gttcttcaat
     8101 ggcagctcca atggcaagag attttgtaaa tgcctgcctc actgacaaaa aacatcctct
     8161 gccaaaaacc ccattcccac ctgcaccaaa aggcatgact ggaagtattt tctgtaaaac
     8221 atccggcgag cttgtcaatg gtttttgccc aagggaccag attagcagcg attatttctt
     8281 tgccgggacc acacctgaca ctccatgcca ataccacaaa cagggcgttt tcccatacaa
     8341 cgttgttgaa gaaattgttg acgggaaaaa atattattat aaacccggaa actcctggtg
     8401 tgtgacaaaa gaagttctcc tttcaaaaga tgtttacgaa aaactccaga caaaatctga
     8461 ttgtccggca ttgatcaacc ttaccatctc cgaccaatca ggccagctga tgcaggagcc
     8521 atactcactt tccaagggca atatctacaa ccttgatgtt tccataccac aggatgtcct
     8581 tggaaaattc aacgaaatag atgtctattt tggtgatatg agggtaaaga aatggcaaga
     8641 gggtgatctt gattccgata caggtcaaaa agtggaattc agctcaccgc tcaatgccat
     8701 gtttgctgtg tctccagatg atgcagacaa aacaaaaaaa ttgatttttg agataagggg
     8761 cccaggcggc ttttatctca aaaaaacttt tgatgtgggg ataagctagg ctggcgatgc
     8821 tcccagttcc actaccaaga acaaaaacga aaaaacccag aggattcccg tttctgggtt
     8881 ttttcgtgct tattattctg gtggtagttt tttctgcggc cttctacctt gcgttcattg
     8941 tttcagacta tgatgtcagg tggctgccca caatccttga cccacaaaac cataaaccgc
     9001 caaatcccca cgagactgta aatgaaacaa acaggatctt tgatgccgag gggaacctta
     9061 tccatgagtt ccatggcgat gaggacagga tatatgtccc gctcgaacaa atttccgaat
     9121 gggccagaaa agcaatcatc gcgtctgaag atgtaaggtt ttattaccac aagggttttg
     9181 acatgagggc cacactgaga gcatttttca aaaatgtcca gacgcgagaa gcctctgagg
     9241 gtggctcaac tatcacccag cagcttgcca gaaacatgta ccttggaact gggaaaaccc
     9301 tttcaaacga gaggactttt acaagaaagc taaaagagtt gctgctggct cgaaaaatag
     9361 agcaagccta ctctaaaaat gaaatcctcg aattctacct gaatcaggtc tactttggct
     9421 caagggccta cggaatagag tctgcggcaa agagatattt tggcattcca gccaaaaacc
     9481 ttacgcttgc acagtcatcg atgcttgcag gcgtcctgtc ttcgccatca tttaataacc
     9541 cgtatgacaa tctggcagag gccaaaaaag cgcaggaagt tgttctcgaa aagatggcca
     9601 aaaatggttt tataaccgat gtccagatgg aaaaggcatc aaaggagaag ctcaatttcc
     9661 ggccatattc catccagctt aaggctggcg attccctagg ttatttcata gattacgtca
     9721 aagacgagct ttacgacaaa cttggagagt tcccaatcaa aaagggtggc ctagacatat
     9781 acacaaccat aaaccaggac tgccaaaagt atgccgttca agccgttaac aaagtccttg
     9841 gagaagccat aaaagctggt gattttggca aaattcaaaa agataagctt ggagttgaac
     9901 agccgcaggt ggccctttgt gccattgatg ccgatactgg ccagatacag gcaatggttg
     9961 gcggaaggga ctacaagaac acccaataca acaggtgcac agcgttaaga cagcctggct
    10021 cttcatttaa aatacttgtt tacagcgccg cactccagca tggcgtcctc tcaccatcaa
    10081 gtctggtccg ctccagcccg atttcaattg gtgactggca gccaaaggaa tggttcaggg
    10141 gatattttgg aactgtaaca gtcaaatatg ctctccagga atcaagcaat atctgtgcaa
    10201 taagggcagc tctgaaggtc gggcttggca atgttgtcgc gacagccaaa aaaatgggca
    10261 tcagcacatc aaaattgctt gccgtgccat ctattgccat tggctatgta gaggtcaaac
    10321 ccatcgaaat ggcttccgtt ggccaaacta tttcaaacta tggaacccac ataccaccaa
    10381 ttgccatcaa gaggatagag aacagaaaaa caggcaagac cattgaccct gcctatgggc
    10441 aaccaaccag ggcaatcgat gagccaacag catacgacat gatctcgatg ctgaagaatg
    10501 ttgttgagcg aggcactggg atgtcggcca aagtgccagg cgtcccgtgc gctggcaaaa
    10561 caggaactac aggctccttt agagatggct ggtttgttgg ctttacccct gaaatctctg
    10621 cctcggttta ccttggctct gactccagag aggttgattt gtcatttgtt tccaactatg
    10681 gatcaaaata ttcagcacgc atctggaaag agtttatttc gatgtattca aagggcagga
    10741 cgatcaagga ctgggtggac aaaaaccctg atgattacgt cagagtgagg gtctgtgagg
    10801 aaactggtct tatagcaaac gacacctgcc cgggctatta catgcttgta agaaagagca
    10861 agatgcccag tgagagat
//
LOCUS       DAOSRI010000022        10804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_5972_length_10804_cov_4.044004, whole genome shotgun sequence.
ACCESSION   DAOSRI010000022 DAOSRI010000000
VERSION     DAOSRI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5972_length_10804_cov_4.044004"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /gene="leuS"
                     /locus_tag="PK717_01235"
     CDS             complement(<1..2161)
                     /gene="leuS"
                     /locus_tag="PK717_01235"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOR46327.1"
                     /translation="MGNFDFKAIESKWQKYWDDNKIYRTTNDPGKKKLYCLDFFPYPS
                     GSGLSVGHCRNYVPSDVYSRYFRMIGYNVLHPMGWDSFGLPAENYAIQMGIHPEATTA
                     KNTANYKRQMTLIGTSYDWEREINSSDPDYYKWTQWFFLLLFERGLAYQNYGAQWWCP
                     ECKTVLANEQAEGGVCWRCGSQVERKDLKQWFFKITDYADRLEKDLETIDWPEPIKIM
                     QRNWIGRSEGAKIIFKSEKGRDIVVFTTRPDTLFGCTYMVLAPEHELVPELTTPEQKN
                     AIEAYIKEARKASDIDRLSTDKEKTGVFTGSYAINPANGKKVPIWIGDYVLATYGTGA
                     IMCVPAHDERDFAFAKKFGLPVVQVISKDGGLVNLENAYTEPGTMVNSGQYDGMDSEK
                     FKKVLVEELGKKGIATPTVNYKLRDWLISRQRYWGAPIPVIHCEKCGTVAVPKDQLPV
                     KLPHIDKFEPSTDGKSPLAKIPEFVNTTCPKCGGPAQRETDTMDGFACSSWYFLRFPN
                     PHENDRPFNPEMIKYWLPVNVYVGGAEHAVMHLIYARFWTKVMHDAGLISFIEPFQKL
                     MNQGMVLGGDHQKMSKSKGNVVTPDSMVEKYGADALRGYILFMGPFEGEVVWDEPGCA
                     GVYRFLGRVFDNFTSWKYGNISASEQLTKELTRIKHKLILKVTSDIERFHFNTAISSF
                     MEAFNSIYDLAKTPGTTETKEFRDFIENYIVLLAPFCP"
     gene            complement(2221..2296)
                     /locus_tag="PK717_01240"
     tRNA            complement(2221..2296)
                     /locus_tag="PK717_01240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2261..2263),aa:Ala,seq:ggc)
     gene            2405..2481
                     /locus_tag="PK717_01245"
     tRNA            2405..2481
                     /locus_tag="PK717_01245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2439..2441,aa:Ile,seq:gat)
     gene            2499..2574
                     /locus_tag="PK717_01250"
     tRNA            2499..2574
                     /locus_tag="PK717_01250"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2532..2534,aa:Ala,seq:tgc)
     gene            2764..4092
                     /locus_tag="PK717_01255"
     CDS             2764..4092
                     /locus_tag="PK717_01255"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HOR46328.1"
                     /translation="MMNSFERIQRVINYIEENLTVRINPDTACGQSGISTAHCYRMFH
                     MLVGRSLMAYVRTRRMTEAAKKLRAGHESIIELALDCEYDSQEAFTRAFKSEFGVTPG
                     TFRQKKPKIKEYSKVDLIEKYYDNSANMQGDPKVKVLKWLPPIRVAYYNAIGKTPEKD
                     AWNKLLDWAASNGLFDGPYRLFGFNNPSPQPGKDEYGYEVCITVEKDVAGTDEIKFKH
                     LMGGHYAVMSTTLPNIEKDWKHFSTWLSLSKYEYGTHQCLEEHLTPPDRWDSEALEID
                     LYMPIKIKEKPMEKEIREVKLDKMRVAHCCALSTSPETDSWKIMKEWATKNGILDLPG
                     TKIFGFDNPCPEPDRSFYGFEHWVTVPDGVESSGGVGIKEVEGGDYLILSSRLEDIPE
                     NYKRLFKSFDEKGIDCRHAPWIQELIFDKEDPDNQDLMELVLYAPFKRKN"
     gene            complement(4160..5194)
                     /locus_tag="PK717_01260"
     CDS             complement(4160..5194)
                     /locus_tag="PK717_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR46329.1"
                     /translation="MTKKLAPITLALVLAIVFFASCEGGSSKLSGTVRDTISLAKIAN
                     ASIVIDSKTIVTDDDGRFIVEGLSEGKFDVIISADGYSKTTYPEFEIKDGDNFLEATL
                     SKTDDSKAGEITKTPLKPQTTPPPAPGKPVGNRPNINIYMDFTNCVVKTTKGDKQFPG
                     SILEMTFNGDSVRNVIKSQNPQAPQTEIIFTPTTTYNKVPSQGWIGIKVPKGQAPGES
                     SKIQLQLYMKAIKEVLSDKNSTFNALGKGSFASQEVEKYYIAGYGKDATDQVDGEILV
                     LSKGSNKGMMVSFNGKLTIGSEGDSYTIEITNIGSAPKIQIPENAKIMDPSSSTQLKP
                     SQPISKPKNP"
     gene            complement(5191..5835)
                     /gene="rpe"
                     /locus_tag="PK717_01265"
     CDS             complement(5191..5835)
                     /gene="rpe"
                     /locus_tag="PK717_01265"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOR46330.1"
                     /translation="MTKLAPSLLAANPLNLEEGIKNINEFADILHLDVMDGHFVPNLA
                     LGILTCESACKVSLVPAYAHLMVNNPGEYIEKFRNMGAKMFVWHVELGLKHMEIINLV
                     KSSGMKPGLAIKPDTDCDILKPFIDQIAIVTVMGVYPGFSGQKFIPSTINRVAKVREL
                     SETIEIEVDGGVGLENASALVENGADILVSGSSFFNSKDKKTFSDQIRSLRRKP"
     gene            complement(5832..6518)
                     /locus_tag="PK717_01270"
     CDS             complement(5832..6518)
                     /locus_tag="PK717_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOR46331.1"
                     /translation="MKKGLLISTIIVFTMFFAGCGGPDLMIMVPNLTNMRFQTAEKVA
                     QKRDLKLVIEKYEASEFVPEGSIISQDPSEGAYIKTARSVFVVVSSGKRKMKVPNLIG
                     MKYGDAQDSLLKIGLAIGGIEEESSNASDVGYVVRQFPAPDSEVDRNTPITLTVSVGQ
                     LTTVPNVLGLPVDQASETLQQAGFEVNLIAVQKNLAPYADANIVLRTDPEPNRECDTK
                     NKIDIYFRQP"
     gene            complement(6546..8183)
                     /gene="uvrC"
                     /locus_tag="PK717_01275"
     CDS             complement(6546..8183)
                     /gene="uvrC"
                     /locus_tag="PK717_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOR46332.1"
                     /translation="MTELEEKVKNLPLKPGVYLFKDSTGKVIYVGKAKCLRDRVSSYL
                     DKSQLLPKVAAMMDHACDLDFIVTYSAHEALVLENNLIKEYQPHYNIKLRDDKEYSFI
                     FVSKESFPRLGKTRRTDNPNFHYFGPYTRSESLATNIRTIRQFFPVRTCKLELPEKKC
                     RPCLDYHIGLCLAPCASLTSQEEYQSMVADLLLLLSGKYKKLEETLHKKMTEASASLR
                     FEEAARLRESIKNLSSIVSKQRVISTEPADRDAFAVFNNENRACVEYMKVRGGRLILD
                     MQMFAGSEDEATPSEFLGAVMQQVYGSQDNIDLPSEILVNVKPEGEKELFEFINQGRE
                     LLRQIKIVQPKRGDKVQLLKMAETNAKHHLSEHMRRETLALGKNAITQLQEALELKKP
                     PFLIEGYDIANIQGKGAVGSQVSFKDGRPNKKGYRIYKIKTKDTPDDYAMMRETLERR
                     RTHWDDEEFAQKPDLLLIDGGIGQLNVALDVFKGMDIEIASLAKEEELVMRKGMEPIR
                     LPETSLALRLLQQIRDESHRFAGKHFRTQHKKLSGLD"
     gene            complement(8183..8389)
                     /locus_tag="PK717_01280"
     CDS             complement(8183..8389)
                     /locus_tag="PK717_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46333.1"
                     /translation="MNEMTDEAEFVVDILKGKGKMTTTQIEDAIKAQGIACRDAASRF
                     LPGLRAQGFIKGEFKSKTWVWWVD"
     gene            complement(8386..>10804)
                     /locus_tag="PK717_01285"
     CDS             complement(8386..>10804)
                     /locus_tag="PK717_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR46334.1"
                     /translation="FGLEISENYESVRVWLKKEPQPLLSLDWPSIDLVKMNDKKVAKP
                     FEANLYPIRENAKYSLLQDQSQYKLFFFYSNNQACAKMIPFIESTFRRYKDKGLSCYL
                     VSNSIPTDSTVEQFFAGNRDIMEGLDALSIVGLTLPIMFNESGEICEGFPGESIPRLY
                     IVNSKDELVFALPDANQSQLIYLEQATASIFDKQGAVPPDVLTVNVSNKGGSAGQGSI
                     TSNSPLVNILTGTFRSSPVQVSLSFDPSQVELENTPNYKIATATLLGTSNQVEIPVRC
                     WNIPPFTSFSSWYNGSKTIRINGSNVSMPVAATAGDDPVGPIGLIAKSIGAEVRILSN
                     QTVRFIAGQNEATMYIGQNFAESGGSKFEFGGKFSIESGEIYGPVKFLSQFAGATLQS
                     FDGTIVLWAPTIRGRATTPLLSVDKSSLMFNSYIPPAGGFRAAPDFELPSYPYSSGAK
                     TKLSELLKKPEVQVVIIDFWATWCPPCKSSMPHLEKLWRNYKNKGLVVLGVITDQDEK
                     TDEDIEETLTEDSRIKGGLKSLGLDKITYPMCYDIRPELKTFRLYEGKAIPRLVVITK
                     DMKWAYTNVGFWDQGVRNLEFKVRRLLGIEESAKAPSLSLSNIGVGELSGTITSSLPY
                     ITPKNTNFSTTDSTSIEFSVNTWTAPVFPETGTITIESNGGKFICDIQYNPVFENKPL
                     ELKINTTDSSLTFNNLKTKVSISTKTFGDKYLVAINSLLPALGGSASVLPDRKRALGQ
                     FETWQISVEHNKTAVSAGPFTLNAQAPTIIEGGDIFVDFETLCDIIGAEASFENENKT
                     LVLRYEI"
BASE COUNT         2635 a         2370 c         2457 g         3342 t
ORIGIN      
        1 acgggcaaaa tggcgcgagc aggacgatat agttttcgat gaagtctctg aattcttttg
       61 tttctgttgt acctggtgtt ttggcgaggt cgtaaattga attgaaggct tccatgaatg
      121 aagatattgc agtgttgaag tggaaccttt cgatatctga tgtaaccttc aaaatgagct
      181 tgtgctttat tcttgtcagt tcttttgtca attgttcgct ggcgctgatg tttccatact
      241 tccatgatgt gaaattatca aagaccctcc caaggaacct gtagacgcct gcacaaccag
      301 gctcgtccca gacaacttct ccttcaaacg ggcccatgaa aagaatgtat cccctcagcg
      361 cgtccgcacc gtatttctcg accattgagt ctggtgttac gacattgcct tttgatttgc
      421 tcatcttctg gtggtcgcca ccaagcacca tgccctggtt catcagtttc tggaatggct
      481 cgatgaatga gataagacct gcatcgtgca ttaccttagt ccagaacctt gcatagataa
      541 ggtgcattac ggcgtgttca gcgccaccaa cataaacatt gacagggagc cagtatttga
      601 tcatttcagg attaaatggt ctatcatttt catgaggatt ggggaacctc aggaaatacc
      661 atgaggaaca agcaaaacca tccattgtgt cagtttccct ttgtgctggt ccgccgcatt
      721 tggggcaggt tgtgtttacg aattctggaa tttttgccaa cggggacttt ccatctgtgg
      781 atggctcaaa tttgtctata tgtggaagct ttaccggcag ctggtctttt gggacagcta
      841 cagttccaca tttctcgcaa tgaatgacgg gtattggtgc tccccagtat ctttgtctgc
      901 tgatcagcca gtctctcagc ttgtagttta ctgttggggt tgcgatgccc ttttttccga
      961 gttcttcaac gaggaccttt ttgaatttct ctgaatccat gccatcatat tgcccggaat
     1021 tgaccattgt ccctggttcg gtataggcgt tttcaaggtt cacgagtccg ccgtctttgg
     1081 agattacctg gaccactgga aggccaaatt tctttgcaaa agcaaaatct ctttcatcat
     1141 gggctggaac acacatgatt gcacctgttc cataggttgc caggacgtaa tcgccaatcc
     1201 atatcggcac ttttttccca tttgctggat ttattgcgta agagccggtg aaaactccgg
     1261 ttttttcctt gtcagtggag agcctgtcta tatcggaggc ctttcttgcc tcttttatgt
     1321 atgcttcaat ggcatttttc tgttcaggtg ttgtcagttc tgggacgagc tcgtgctctg
     1381 gcgcaaggac catgtatgtg cagccaaaaa gagtgtcagg gcgtgttgta aaaacaacaa
     1441 tgtcgcgccc tttttctgat ttgaatataa tctttgcgcc ttcagatctg ccaatccagt
     1501 ttctctgcat aattttgatt ggctctggcc agtcaatggt ctccaggtcc ttttcaagtc
     1561 tgtctgcata gtcagttatt ttgaagaacc actgtttaag gtcttttcgt tccacctgcg
     1621 aaccgcacct ccagcaaacg ccgccttcgg cctgctcgtt tgcaagaacg gtcttgcatt
     1681 ccgggcacca ccactgggcg ccgtaattct ggtatgccaa tcccctttca aagaggagca
     1741 ggaagaacca ctgtgtccac ttgtagtaat ctggatccga ggagttgatt tccctttccc
     1801 agtcataact ggtgccgatg agggtcatct gtcttttata attggcagtg ttttttgcag
     1861 ttgttgcttc agggtgaata cccatctgta tggcatagtt ttccgcaggg agcccaaacg
     1921 agtcccagcc cattggatgt agcacattgt agccaatcat cctgaaatat ctggagtaaa
     1981 catctgatgg aacataattc ctgcaatgcc ctactgaaag accgcttcct gatggatatg
     2041 gaaagaaatc gaggcaatat aatttctttt ttccaggatc atttgtggtc ctgtaaatct
     2101 tgttgtcatc ccaatacttc tgccatttgg attcgattgc cttgaaatca aagtttccca
     2161 ttgctggcgc ctccttatgc aattaaagcc tccttgcgga ggctttttgg cgtcttttac
     2221 tggtggacct gaggagattc gaactcctga ccttctgcat gccatgcaga cgcgcttcca
     2281 gctgcgccac aggcccacgt tgggatattt ataacgatgc ctaaatgaaa gtcaaggaac
     2341 aatagttcta ctggttttcc acccttgaca tggtatttgg tgacattata ctaaccgaat
     2401 tgttgggcct ctagctcagt tggttagagc gcgtccctga taaggacgag gtcagtggtt
     2461 cgagtccact gaggcccacc atgcagctct ttgcaagtgg ggatgtagct cagctggcag
     2521 agcatcgcgt ttgcaacgcg agggtcgggt gttcgaatca cctcatctcc accataaaat
     2581 cccccactaa gttgaactga ccatctatca ttttttagaa ataatcccaa gaataaatcc
     2641 gttgcaggaa aaaaatttct ttctgctatt tgtgcaaatt gagtattgag ttttttgtta
     2701 aaccggtgat agaaataatc aagacaagag aacagatggt gtgaataatg ggtggtggtg
     2761 acgatgatga acagctttga acgaatccag agggtgatca attatattga ggaaaacctc
     2821 acagtccgga taaatccgga cacggcctgc ggccagtcag gcatctccac cgcccattgc
     2881 tacagaatgt ttcatatgct ggtgggaaga tccctcatgg cctacgtgag gacaagaaga
     2941 atgactgaag cagcaaaaaa gctgagagct ggacatgaaa gcatcatcga gcttgccctt
     3001 gattgcgagt atgactcgca agaagccttc acaagggcct tcaagtctga attcggtgtt
     3061 acaccaggca cattcaggca aaaaaaacca aagataaagg agtacagcaa agtggacctg
     3121 attgaaaaat attatgataa ttcggcaaat atgcagggag accctaaagt taaagtcctg
     3181 aaatggcttc ctccaattag ggttgcctat tacaatgcga ttggcaaaac accggagaaa
     3241 gacgcttgga acaagctttt agattgggca gcaagcaacg gtctttttga tggcccatac
     3301 agactttttg gattcaacaa cccttcacca caaccaggaa aagatgaata tggctatgaa
     3361 gtttgcatca ccgtggagaa agatgttgca ggaactgatg aaataaaatt caaacacctc
     3421 atgggaggtc attatgctgt catgagcaca acactcccca acatcgagaa agactggaag
     3481 catttttcaa cgtggctctc gctgagcaag tatgaatatg gaacgcacca atgccttgag
     3541 gagcatttga cgcctccgga cagatgggac agtgaagcac tcgaaatcga cctttacatg
     3601 ccgatcaaga tcaaggaaaa acctatggaa aaggaaataa gggaagtaaa gctggataaa
     3661 atgagggtag cacattgctg tgccctgagc acttcaccag aaacggattc ctggaaaata
     3721 atgaaggaat gggcaactaa aaatggaatt cttgatttgc caggaacaaa aattttcggt
     3781 tttgacaatc cgtgtccgga acctgacaga tcattttatg gatttgagca ttgggtcact
     3841 gtaccggatg gcgtcgagtc ttctggtggt gttggtataa aggaagtcga gggtggtgac
     3901 taccttattt tgtcaagcag actcgaggac attcctgaaa attataagag actcttcaag
     3961 agttttgatg agaaggggat tgattgcagg catgctccgt ggattcagga acttattttt
     4021 gacaaagaag atccagacaa tcaagatctt atggaacttg tcctttatgc tccctttaag
     4081 agaaaaaact gaattatggt gaaatttaaa aggccagata aatattacct ggcctttttg
     4141 tttttctagg tttttgttat cagggatttt tgggttttga tattggctgt gatggtttta
     4201 gctgggtaga agatgatgga tccattatct tggcattttc gggtatctga atttttggtg
     4261 cagagccaat attggttatt tcaattgtat agctgtcacc ttcgctgccg atggttaatt
     4321 tcccattgaa ggaaaccatc atacctttat tagatccttt tgaaagtaca aggatttccc
     4381 catcgacctg atctgttgcg tccttcccat accctgctat atagtatttt tcaacttcct
     4441 gactggcaaa agatcctttt ccgagagcgt tgaatgtcga gtttttgtca gataaaactt
     4501 cctttatggc cttcatgtaa agctggagtt ggatttttga agattctccg ggagcttgtc
     4561 cttttggaac cttaatgcct atccagccct ggctcgggac cttattgtac gttgttgttg
     4621 gggtgaatat tatttcagtt tgtggtgctt gaggattttg gctttttatg acatttctta
     4681 ccgagtctcc attgaatgtc atttcaagta ttgatccggg gaattgtttg tcaccctttg
     4741 ttgttttgac tacacaattt gtaaaatcca tataaatatt tatgtttggc ctgtttccca
     4801 caggttttcc tggagctggt ggtggggttg tttgtggttt cagtggagtt tttgtgatct
     4861 ctcctgcttt ggaatcatct gtttttgaga gcgttgcctc aagaaaattg tccccgtctt
     4921 taatttcgaa ctcggggtag gtggtttttg aatacccgtc tgcagagatt atcacatcaa
     4981 acttgccctc ggagagtcct tccacaataa atcttccgtc atcatctgtc acgattgttt
     5041 tcgaatcgat tacaatgctt gcatttgcaa tctttgccaa gctgattgta tctcttacgg
     5101 tgcctgatag ttttgatgat ccaccttcgc aagaagcaaa gaatactatc gctaaaacca
     5161 gagccagtgt tatgggggct agtttttttg tcatggtttc ctccttaaag accttatctg
     5221 atcagagaag gtctttttgt cttttgagtt aaaaaaactt gatcctgaaa cgagaatgtc
     5281 agcgccattc tcgacaagtg ctgatgcgtt ctcaagccca acgccaccgt caacctctat
     5341 ttctattgtt tctgacagct ctctcacttt ggcgaccctg tttattgtgg atggaatgaa
     5401 tttttggcct gaaaatccag ggtaaacacc cattactgtt acgatggcta tctggtcaat
     5461 aaatggcttg agaatatcgc aatctgtgtc gggttttatg gccagacccg gtttcattcc
     5521 cgaagatttg acgagattta ttatttccat atgttttagg ccaagttcta catgccatac
     5581 aaacattttt gcacccatgt tcctaaattt ttctatgtat tcgccaggat tgttgaccat
     5641 cagatgtgcg tatgcaggaa ctagtgaaac tttgcatgca gattcacatg ttaggattcc
     5701 caaagcaaga tttgggacaa aatggccatc cataacgtca agatgaagaa tgtccgcgaa
     5761 ctcgtttata tttttgatcc cctcttcaag gttgaggggg ttggctgcaa gaagtgaagg
     5821 ggccagtttt gtcatggctg cctgaagtag atatcaatct tgtttttggt gtcgcattcc
     5881 ctgtttggct caggatcagt tctcaaaacg atatttgcgt cagcgtatgg ggcaaggttt
     5941 ttctggacag ctataaggtt tacctcaaaa ccagcctgct ggagagtttc ggaagcctgg
     6001 tcaactggaa gacccaaaac attggggaca gttgtcagct gtccaactga tacagtcagt
     6061 gttattggag tgtttctatc aacttcgctg tctggagccg gaaattgcct gacgacataa
     6121 ccaacatcac tggcattgga tgattcctct tctatcccgc ctattgcaag ccctatcttg
     6181 aggagtgaat cttgtgcatc tccatatttc attccaatga ggtttggcac tttcattttt
     6241 ctcttgccac tcgagacaac gacaaaaacg ctcctggcgg tttttatgta agcgccttcg
     6301 gaaggatcct gtgatataat gcttccttcc ggaacaaact ctgaggcctc gtatttttca
     6361 atgaccagtt ttagatctct tttctgggcg accttttctg cagtctggaa cctcatattt
     6421 gtgaggttag gcaccattat catcagatcg ggaccaccgc atccagcaaa aaacattgtg
     6481 aagacaatta tggttgaaat aagcaaacct tttttcatgg tgtttaagtt attccctttt
     6541 ggcctttaat caagtcccga cagttttttg tgctgtgttc tgaagtgttt tccagcaaac
     6601 ctgtgggact cgtctctaat ttgctggaga agtctcaatg ccaggctggt ttccggcaac
     6661 cttatcggtt ccatcccctt tcgcataacg agctcttctt ccttggcaag agaggctatc
     6721 tctatatcca ttcccttgaa cacatcgagg gcaacgttga gctggcctat tccaccatca
     6781 atgaggagga ggtctggttt ttgggcaaac tcctcatcat cccaatgtgt ccttcttctc
     6841 tccagcgttt ctctcatcat tgcataatca tcaggagtat cttttgtttt gattttgtag
     6901 atcctgtagc ctttcttgtt tggtcggcca tccttaaagc tgacctgcga cccgactgca
     6961 ccttttccct gtatgtttgc tatgtcgtaa ccttcaatga gaaacggtgg ttttttaagt
     7021 tcaagtgcct cctgaagctg ggttatagca tttttgccaa gtgcaagggt ctcccttctc
     7081 atgtgctctg aaagatggtg ttttgcattt gtttctgcca tcttcagcaa ttgcaccttg
     7141 tcaccccttt ttggctggac aatttttatc tgcctgagga gttccctacc ctgattgatg
     7201 aactcaaaga gttccttttc accctctggt tttacattta caaggatttc tgatggcaaa
     7261 tcgatgttgt cctggctacc gtatacctgt tgcatcactg caccaagaaa ctctgaaggg
     7321 gtggcttcgt cttctgaacc tgcgaacatc tgcatgtcca ggattaacct gcctcccctt
     7381 actttcatgt attcgacgca cgccctattt tcgttattaa aaacggcaaa agcatctcta
     7441 tcagctggtt ctgtgctgat gactctttgt ttggatacta tcgaggagag gttcttgata
     7501 ctttctctca gcctggcagc ttcctcgaac cttaaggacg cagatgcttc agtcattttt
     7561 ttgtggagtg tttcttccag cttcttgtat ttgccagaaa gtaacaagag caaatcggca
     7621 accattgact ggtattcttc ctgacttgtt aggctggcgc atggcgcaag gcacaatccg
     7681 atatgataat cgaggcaagg cctgcatttt ttctctggga gttcgagctt gcacgttctg
     7741 accggaaaaa attgccttat agtcctgata tttgtcgcaa gagactcaga tcttgtataa
     7801 gggccaaagt aatggaaatt tggattgtcc gtccttcttg tcttgccaag ccttgggaat
     7861 gattctttcg aaacaaaaat gaagctgtat tccttatcgt cacgtagctt gatgttgtag
     7921 tgtggctgat attcctttat cagattattt tcgagtacga gggcctcgtg ggccgagtag
     7981 gtgactatga aatctagatc gcatgcatgg tccatcattg ctgccacttt tggcaacagc
     8041 tgcgatttgt ccagatatga cgaaactctg tcgcgaaggc attttgcctt tccgacgtat
     8101 atgaccttcc cggtagagtc cttgaagagg taaacaccag gcttgagtgg aaggtttttt
     8161 accttctctt caagctccgt cattaatcaa cccaccacac ccacgttttg cttttaaact
     8221 cacccttgat gaagccctga gccctgagcc cgggtaggaa tcttgacgcc gcatccctgc
     8281 atgcaatccc ttgggccttg atggcatcct caatttgggt ggtggtcatt ttgccttttc
     8341 ccttgagaat atctactaca aattctgctt catccgtcat ttcgttcata tttcatacct
     8401 cagtaccagt gttttgtttt cgttttcaaa agatgcctct gctccgatta tgtcgcagag
     8461 cgtttcaaag tccacaaaga tgtcaccacc ttcaatgata gttggggcct gtgcgtttag
     8521 ggtgaatggt cctgcggata cagctgtttt gttgtgttcg acacttattt gccatgtttc
     8581 aaactggccc agtgcccttt tcctatctgg aagcaccgaa gctgatccac caagtgccgg
     8641 aagcagcgaa ttgatcgcga ccagatattt gtcaccaaaa gtttttgtgg aaatagaaac
     8701 cttggttttc agattgttga atgtaagaga gctgtcagtg gtgttgattt ttagctccag
     8761 tggtttattt tcaaaaacag gattgtattg aatatcgcaa ataaattttc caccattgga
     8821 ttcaatagtt attgtccccg tttccgggaa tacaggagcg gtccaggtgt tgactgagaa
     8881 ctcgattgat gtggagtcag tggtggagaa atttgtgttt tttggcgtta tgtacgggag
     8941 ggaactggtg attgtgccgg agagttcccc aacaccaata tttgaaaggc tgagggatgg
     9001 ggcttttgcg ctctcctcaa tcccaagcaa tctccttacc ttgaattcaa ggttcctcac
     9061 accctggtcc cagaaaccca catttgtata tgcccatttc atgtcttttg taataacgac
     9121 aagccttggg attgcctttc cttcgtaaag gcggaatgtt ttgagttctg gccttatgtc
     9181 atagcacatc ggataagtta ttttgtccaa gccgagtgat tttaggccac ctttgattct
     9241 tgagtcctca gtgagtgttt cttcaatatc ttcatctgtt ttttcatcct ggtcagtgat
     9301 gacaccaagc accaccaggc ctttgttttt gtaatttctc caaagcttct caagatgggg
     9361 catggaggac ttgcatggtg gacaccaggt tgcccagaaa tcaataatga caacttgcac
     9421 ttctggtttt ttgaggagtt cggagagctt tgttttggct cccgaggagt aggggtagct
     9481 tggaagctca aaatctggtg ctgccctgaa cccacctgct ggcgggatgt atgaattgaa
     9541 catcaggctg gacttatcaa ccgagaggag tggggttgtt gcccttcccc ttattgttgg
     9601 tgcccacaac actattgttc cgtcaaaact ctggagggtt gcccctgcaa attgtgaaag
     9661 gaactttaca gggccgtata tttctcctga ttctatcgag aatttgccac caaattcgaa
     9721 ttttgatcca ccagattcgg caaagttttg tccgatatac attgttgctt cattttgccc
     9781 ggcaataaac ctgacagtct ggttggagag gattcgtact tctgcgccta tggattttgc
     9841 aatcagccct attggtccca ccggatcatc acctgcagtg gcagcaacag gcatgctgac
     9901 gtttgagcca tttattctta tggtctttga accattatac cagctggaaa aggatgtgaa
     9961 aggcggaatg ttccagcacc tgacaggtat ctcgacctgg ttgcttgttc caagcagagt
    10021 cgctgttgcg attttgtagt ttggtgtatt ctcgagttca acttgagatg gatcaaagct
    10081 caatgaaacc tgtacaggtg aactcctgaa agtaccagtc agaatgttga ctagtggtga
    10141 gtttgatgtt atcgagccct ggcctgcaga gccacctttg tttgacacat ttactgtgag
    10201 cacatctggt gggacagcgc cctgtttgtc aaagatcgaa gcggtggcct gttcaagata
    10261 aataagctgg gactggtttg catccgggag ggcaaaaaca agctcgtctt tggaattgac
    10321 aatatagagt ctggggattg actcgccagg gaaaccttcg caaatctcac cgctttcatt
    10381 aaacatgatc ggcagtgtga ggccaactat tgagagtgca tcaagaccct ccattatgtc
    10441 tctattgcct gcaaaaaact gctctactgt agaatctgtt ggtatcgaat tcgatacaag
    10501 gtagcaagac aaacctttgt ccttgtacct tctaaaagtg ctctcaatga acggtatcat
    10561 tttggcacat gcctggttgt ttgagtagaa gaagaaaagc ttgtattgtg attggtcctg
    10621 caagaggctg tacttggcat tctctctgat cggataaaga tttgcctcga aaggttttgc
    10681 caccttttta tcattcatct tcacaaggtc aatgcttggc cagtcgagtg aaagcagggg
    10741 ttggggttct ttcttgagcc agaccctgac actctcatag ttttcactta tttccaggcc
    10801 aaat
//
LOCUS       DAOSRI010000023        10159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6423_length_10159_cov_3.996239, whole genome shotgun sequence.
ACCESSION   DAOSRI010000023 DAOSRI010000000
VERSION     DAOSRI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6423_length_10159_cov_3.996239"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK717_01290"
     CDS             complement(<1..405)
                     /locus_tag="PK717_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46335.1"
                     /translation="MKGKKLLLTLLLAISILGTSFYGVGTSLGYKTEGNIGAIIIDGT
                     PPPVLEPPAREPTSQVQGVAKTLTGVPGFDWCYGCSATSAAMMMGYYDNNGFPDMYKG
                     PTNNGVCPMNNQSWGYGKCPLSATMQSLDQRKN"
     gene            944..1744
                     /locus_tag="PK717_01295"
     CDS             944..1744
                     /locus_tag="PK717_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR46336.1"
                     /translation="MEHSKFTLKDVQDVYSGAHKDLWELVMGEQIHIGGLASSMELAQ
                     RAGIKEGTKGVDLCCCVGAGMRFLARNFKVSMAGVDATDRVLGEAAERAKKEGLEAKL
                     EFKKGDVTAVPYPNSTFDFVWGEDAWCYVEDKGKLVSEAARILKPGGTIAFTDWIEGH
                     NKLTKDEASRIFDFMKFPYMETLEGYKNLLEKNGFEVIEATDQNQYFADHIALYIDML
                     TKQFTYDALRIIGNDANMMQAMGGEMAFMRDMARAGRFSRGRFIGRKK"
     gene            1746..2801
                     /locus_tag="PK717_01300"
     CDS             1746..2801
                     /locus_tag="PK717_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR46337.1"
                     /translation="MKEVFQNPIFIDALEARQSAKNAIKYACSWVPRELLESFGLKTA
                     RLMGCGTPSHESAGERKLSGEVCSFCKTIAGASLSGAPVIGCTSCDQMRRLSEIILSE
                     KYETGFMVNVPKTCTDNSRELYRSEIRALYSHLESLTGKSLTDESFNTKLKNVCDKRN
                     LIRSKLRSLRKSLSAVDFHCLVHLESQTDPDFMMSYLEKPFKTKTSLKKKTMLAGSPL
                     ALEDAAFLKLLEDSGFNIIFNATCTGDRSIDFDLSIKDDPITDLADSYFNHPPCIWKR
                     PNDSFYSYVSKKLLETGATSVIWRSVRYCDLWNIEAQRAKQIINLPMLVLDMNYSDTC
                     SPRTATRVEAFRESLPQ"
     gene            2798..4249
                     /locus_tag="PK717_01305"
     CDS             2798..4249
                     /locus_tag="PK717_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR46338.1"
                     /translation="MRQYWPDPKPERITYDQWNSQFRMMPESQKKAFCYLEGTSKETQ
                     YLEEPRCFAEDQGDLRLKSLKYDNSLASIRLWSFLLSEEERLHKARLAGKKIIGTLKD
                     LGTMPVLTYSSPDTIAFYPDGAWWIPCIMEMSEGLLKMADSLGLGEEVCPSRATLSAY
                     ISNNHFPRPDYLIAAVGCCCDDFSAIMQRLADMGTKTSWWELPFRGSPSAGEEVELLP
                     TGFYAPKRTADFLEREFDRVRNELSEFLGHPVTDDMVSEGIEKANRFRQIIGEVRDLC
                     YGSAPCPFPALETQICEMIAIHFCSDREEATNVLAHVLETVRKRVESGAGVLPKENCR
                     AVWVNPVADLRVMNLFEDLGGAIAGTEYLFRHALVQIPTNVSPMRALALTALSDPMIG
                     PAWQRAKIVIDEVKKYKAEGIVVSNIPGASHSATEGNIIRELASKEGIPVLQISVPPL
                     TDATSSQLGTRFEAFFELIRSRRKQKWQPTPLA"
     gene            4225..6567
                     /locus_tag="PK717_01310"
     CDS             4225..6567
                     /locus_tag="PK717_01310"
                     /inference="COORDINATES: protein motif:HMM:NF040772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HOR46339.1"
                     /translation="MATYTLGIDLGSRTAKAVLYNIDEKKIASAIVTDTTANSKNDAK
                     RVQELALEYAGVLPSDLKRTIATGYGRGLVDFACDTTTEITCHAVGVHLLVPQARTII
                     DIGGQDSKSIHLNADGTIRDFSMNDRCAAGTGRFLEVVSKLLGTNLDGMAKLAGMATD
                     VPEISSMCVVFAESEVIGMLAKGIRREELSAGIHKSIARRVAGLAERGRIEPPVAFTG
                     GVAKDMAMIRALGAELHEKLLIPPDPRITGALGAAIIAARQLGEKGNLTCTEFKTINL
                     QQSFSDVSQKREEQDMLIVDSIKGDCCPWPPPVKNSSECHHQEEQQVCCGSETQKQAN
                     CNDNEETSCCSSTSVNEAKQSCCGEAKSAQCCEEGQSEPCHSSEKEGTCESGPVKTQQ
                     CNSTTPQGGMYSVPSLSHFDRMIPDCIELARQAKSGGKKVVSIFCEYTPRELILAAGA
                     IPVCACGGNHSMAVASEKELPANLCPLIKSSYGYHTEKANPIFNESDLIVCETTCDGK
                     KKMYELMSKTKPMHILELTQKPDEEAAFEHWLLEVKALKKRLEELTGNEITEERLRAA
                     IRTMNRERTLRSRIADFGGKCLTGMEVLHAKSIISCQERDFKAYEKIISEAENSGDRY
                     SHKPRILLTGVPLPHQAEKVLKLIEEAGAVVVAQENCTGLKPIVDPVSEQGDLLENIA
                     RKYFKLPCSVMMPNKDRFGLLDSLIEKFRPHGVIDLVWQACHTYNIESVLVKKHVQEK
                     WRLPFLKIETDYSPSDTEQLRIRIEAFLSVASQNNLKTSH"
     gene            complement(6634..7236)
                     /locus_tag="PK717_01315"
     CDS             complement(6634..7236)
                     /locus_tag="PK717_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46340.1"
                     /translation="MDKRTETAFVFDGEGNQLQKHVLVWPGRGIYDRFESGWTSRKDG
                     EPQMPSRLYSYAGGTVLLPWEFVDGKWVTGKAIQIDKGGVPADPEMIEGNPKESIRAF
                     GHCGNIILVITWPDFGERTIYIFDQYKRQLSTALYQWSLGSEPPPQFCKGVTGPFLKD
                     FPEGYELVVLKNNKLKTCITVGLAQTMDEDEKIPLLALTL"
     gene            complement(7276..8766)
                     /locus_tag="PK717_01320"
     CDS             complement(7276..8766)
                     /locus_tag="PK717_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46341.1"
                     /translation="MKRVAIFLLAVLLAGCTLAKGESIVDEPLVSFSISVDSTQTNPK
                     IMSSGSFERFIKEDSYTLDDLNKVVTVQTLLNGDVVFWFYQPQYSPKTKILLSANIEG
                     PKCFIYWTHTYESRSNYAGDPIKSEYVPNFSSGTIRVPEGPCLVDYGKDKRSIFICSN
                     GRYKKQDVGMIKRIAPPSGIFFSSSDNSMQLEINLDKTEDEWEDGFVIISKQLLHDMT
                     NQRSNEALLASDLGRVKILRQDGFWFITQIGDYDGCVEDSFYPNPGFYTSNSILKWYC
                     QQKNRLLIDMLLTTMKIAVETTPDVGYKKMPVISELFESWYGLGSDYLDTRFSTDCSR
                     FLINCAQIFDCKGAFEKASMLAKTYERLAPEHTIKFGNGFLFEDYIWFEGTPKIPVHA
                     SLNHTLCEINYLLELYLLNEDESVKQLALKLITGLEETCDSWIRDNGDLWYGYFPAWQ
                     KYDREDYTNLTYNDLQDTSRLFWQVFGQKNWAIEKLLSSKKAYLGK"
     gene            complement(8763..>10159)
                     /locus_tag="PK717_01325"
     CDS             complement(8763..>10159)
                     /locus_tag="PK717_01325"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOR46342.1"
                     /translation="ENGNGNARSVVVAVLSSFCLVVMAVFLTRSGVLSDVSVHSFAGS
                     SMLFLYLVMLLASIGLSVFLCFRSWKFVPHGLESHYTSRYFMMSIGNIVLSVYAVFIL
                     AATLLPVVTQIFGSPAVISQSFYQAISIPVAIIMCVGGALTPLMAWRKTNIKYLARQL
                     IAPLFFGLVFVVLAVEAGMADPLFMITGAFASIMLVSNFQAMLKYRVQKWGAFVSHMG
                     VCLLLMGVVGSSIFFGQESVSLGMSSQNKECLDFGVGLEYVKSDGQSFETMYVLTHKD
                     SEIRGKIGAKWDTRSQVYFSKPFIHRSFLRDVMVYVSNIREDMEFIVGRGSSVLQGGY
                     TVKLLEYNEDVIKALVSDGPDSAKEITFSKDNMAPGSIAEIDGKSYFLSIGAIGNHTV
                     TFKLVDLRNSDRNTVRFTMTVMVKYCMLFVWLGMVITLLGLLWAFLGRLRPTRGNRIS
                     SVFAKDKGAGVALP"
BASE COUNT         3013 a         2328 c         2358 g         2460 t
ORIGIN      
        1 gttctttctc tgatcaaggc tctgcatggt tgcagaaagt gggcatttgc cataccccca
       61 actttggttg ttcattggac acacaccatt attggttggt cctttataca tgtccggaaa
      121 accattgttg tcgtagtaac ccatcatcat tgcagcactt gttgcagaac agccatagca
      181 ccagtcaaaa cctggtactc cagtgagtgt ttttgctacc ccctggacct gggacgtcgg
      241 ctctctggcc ggtggctcaa gaactggtgg tggcgtgcca tcaatgatta ttgctccaat
      301 atttccttcg gttttatacc ccaacgaggt tccaacacca tagaacgatg tgcccaaaat
      361 tgaaatggca agaagcaagg tcagtagaag ttttttccct ttcatataaa acttcctttc
      421 cgtcaactgt atgggattgt tgacatttgt tttaaatata gaaaatttta aataaaagtc
      481 aaattaaatc gttgccagaa tgacgtttat ttgagattag tttttaatcg tgataaacaa
      541 atagatgcag ataaagaatt ttttaatata aatgctgatg aataatccca aattgagaat
      601 aattcttgag tattccaata aaccataagt cttaaaatat caacaaccca tataataata
      661 tcgcacatct tattagaaca aaaaccaaaa acttgattat ttaatcgaac taacatgggc
      721 actcttcttg actttttgat tttttttctg ctattatgca tgcaaattct aaaaagaaag
      781 aggcgggaaa tgtactcaag gaacactaat cgcagaagtc tttgagccgg gcgccaaacc
      841 gtcagtctga cggttccgca taataatccg gacgcgtccc gccagccaaa aatctagatc
      901 ctttttgttt ggtggccgcg ccacacaaaa aggaggaatg gttttggaac acagcaaatt
      961 tacattaaaa gatgttcagg atgtttattc tggggctcac aaagatttat gggagctggt
     1021 catgggagaa caaatccata ttggtggttt agcttcttcg atggaactgg cacagagggc
     1081 cggaatcaag gaagggacga agggtgttga tctgtgctgc tgtgtcggtg caggcatgag
     1141 gttcctggca agaaatttca aggtcagcat ggcaggtgtt gatgcaacgg acagggtcct
     1201 tggcgaggct gcggagcggg ccaaaaaaga ggggcttgaa gcaaaactgg aattcaaaaa
     1261 aggcgacgtc acagcagttc cgtatccgaa ctcaacattc gatttcgtgt ggggcgagga
     1321 tgcatggtgc tatgttgaag acaaggggaa actcgtctct gaagctgcaa gaatattaaa
     1381 accaggtggg acaattgctt tcaccgattg gatcgagggg cacaacaaat tgacgaagga
     1441 cgaggcctca agaatttttg acttcatgaa attcccatat atggagactc ttgagggtta
     1501 caaaaacctc cttgaaaaaa atggctttga agtaattgag gcaaccgatc aaaatcagta
     1561 ttttgcagac cacattgcac tctacattga catgctcaca aaacaattca cttacgatgc
     1621 gctcaggatt ataggaaacg atgcgaacat gatgcaggcc atgggtggag aaatggcctt
     1681 catgagagac atggcccgtg ctggtcgttt cagcagggga aggttcatag gaagaaaaaa
     1741 gtagaatgaa agaagttttc cagaacccca tttttattga tgcgctcgaa gcaaggcaaa
     1801 gtgcgaaaaa tgcaataaaa tatgcctgct catgggtgcc aagggagctc ctggaaagct
     1861 ttggcttaaa aacagcaaga ctgatgggct gtggaacgcc atcccatgag tctgctggag
     1921 aaagaaaatt aagcggagag gtatgttctt tctgcaaaac catagcaggt gcgtcactgt
     1981 cgggcgcacc tgtgattggc tgcacctcat gcgaccagat gagaaggtta tccgagatca
     2041 ttctttcaga aaaatatgaa accggattta tggtcaacgt tccaaaaact tgcacagata
     2101 attcaagaga gctttatcgc agtgaaataa gggcccttta ctcgcacctc gaatcgttga
     2161 ctggcaagtc attgactgat gagtcattca ataccaagct taagaatgtt tgtgacaaaa
     2221 gaaatttaat cagatcaaag ctgaggtcgc tcagaaaatc cttaagcgct gtagattttc
     2281 actgtcttgt ccatctggag tcccagactg atcctgattt tatgatgtct tatctggaaa
     2341 aaccttttaa aacaaaaacc tctttaaaga agaaaaccat gctggcagga agtcctttgg
     2401 cgcttgagga cgcagcattt ctaaaactcc tggaagattc aggttttaac atcatcttta
     2461 acgccacatg caccggcgac agaagcattg attttgatct ttccataaaa gatgacccaa
     2521 ttactgatct ggccgattca tatttcaatc atcccccatg catatggaag aggccaaatg
     2581 attcttttta ttcctatgtt tccaaaaaac tcttggagac aggcgcaact tcagtgatct
     2641 ggagatcagt gagatattgc gacttgtgga acatcgaagc ccaacgggca aaacagataa
     2701 tcaatctgcc aatgcttgtg cttgacatga actactcaga cacctgctct ccaaggactg
     2761 caacaagagt agaggcgttc agggagagtc tgccacaatg agacaatact ggcccgatcc
     2821 gaaaccagag agaataacct acgatcagtg gaactcccaa ttcaggatga tgcctgaaag
     2881 ccaaaagaag gccttttgct atctggaggg aacctccaag gaaacccagt atctggaaga
     2941 accaaggtgt tttgctgaag accagggaga cctgaggcta aaatcgctca agtatgacaa
     3001 ttcgcttgct tcaataagac tctggtcttt cctgctttct gaggaggaga gactccacaa
     3061 ggcaagactc gcaggaaaaa agataatcgg gacactcaag gaccttggta caatgccagt
     3121 cctgacctac tcatcaccag acacaattgc gttttatccc gacggggcgt ggtggatacc
     3181 atgcatcatg gagatgtcgg aagggctgct caaaatggcc gactcgctcg gccttggcga
     3241 ggaagtgtgc ccctcaaggg ccaccctttc tgcctacata tcaaacaacc attttccaag
     3301 gccggactac ctgatcgccg cagttgggtg ctgttgcgac gatttctcgg caatcatgca
     3361 gagactggcc gacatgggaa caaaaacgag ctggtgggag ctcccgttca ggggcagtcc
     3421 atctgccggc gaggaagttg aattgcttcc aacaggattt tatgccccaa aaaggaccgc
     3481 cgattttctg gaaagggaat ttgacagggt aaggaacgag ctttcagagt ttctcgggca
     3541 ccctgtgacc gacgacatgg tttcggaggg tatagaaaaa gccaataggt tcaggcaaat
     3601 aattggcgag gtaagagacc tttgctatgg ctctgcgcca tgcccgtttc cggcactgga
     3661 aacccagatc tgtgaaatga tagccatcca cttctgttca gacagggaag aggcaacaaa
     3721 tgtgcttgcc catgtcctgg aaacggtcag aaaaagggtg gaaagtggtg ctggcgtact
     3781 cccaaaagaa aactgcaggg cggtctgggt aaacccggtt gcagacctga gggtcatgaa
     3841 cctctttgag gaccttggcg gtgcaatcgc cggaacagaa tacctcttca ggcatgcact
     3901 cgtccagatt cccaccaatg taagtccaat gagggccctg gcgttgacag cactttctga
     3961 ccccatgata ggcccggcat ggcagagggc aaaaattgtt attgacgaag taaaaaaata
     4021 caaagcagaa ggcattgttg taagcaatat ccctggcgct tcccacagcg caaccgaagg
     4081 aaacatcatc agggaactgg catcaaaaga gggaattcca gtgctccaga tatcggtgcc
     4141 gcctctcacg gatgcaacat catcacagct gggcacaaga tttgaggcat ttttcgagct
     4201 gataagaagc aggaggaaac aaaagtggca acctacaccc ttggcataga tctcggcagc
     4261 aggactgcaa aagctgttct ctacaacata gacgaaaaga agattgcgtc ggcaatagtc
     4321 accgatacaa ccgccaattc aaaaaatgac gcaaaaaggg tgcaggaact tgctcttgag
     4381 tatgcaggcg tattgccttc tgatttgaaa agaacaattg caaccggcta tggcaggggt
     4441 ctcgtcgatt tcgcatgcga tacaacgaca gagatcacat gccatgcggt tggtgtccat
     4501 ctccttgtgc cacaggcaag aacaattatc gacattggcg gacaggacag caaatctatc
     4561 catctcaatg ccgatggtac cataagagac ttctccatga acgacaggtg cgcggcagga
     4621 acagggagat ttctcgaagt ggtttcaaag ctcctcggga ccaacctcga tggaatggca
     4681 aaactggccg ggatggcaac agatgtacct gagataagct caatgtgcgt cgtctttgca
     4741 gaaagcgaag tgataggaat gctcgcaaag ggcatcagga gagaggagct ttcggcaggc
     4801 atccacaaat caatcgccag gagagtcgct gggctcgcag agagaggaag gattgaacct
     4861 ccggttgctt tcaccggcgg tgttgccaaa gatatggcaa tgataagggc gctgggcgca
     4921 gagctccatg aaaaactgct cattccgcca gacccaagaa taacaggcgc actgggtgcg
     4981 gcaatcatag cggcaaggca actcggcgaa aaaggtaatc tcacttgcac cgaatttaaa
     5041 accatcaacc tccagcagtc attttcagat gtgtcccaaa aacgggagga acaggacatg
     5101 ctgattgttg acagcataaa gggcgattgt tgcccgtggc caccaccagt aaaaaattcg
     5161 tcagagtgcc accaccagga ggaacaacag gtttgctgtg gctctgaaac ccaaaaacaa
     5221 gcgaattgca atgataatga agaaacatca tgttgcagct ctacgagtgt gaatgaagca
     5281 aaacagtcat gttgcgggga agcaaaatca gctcaatgct gtgaagaggg ccagtcagag
     5341 ccatgccact catctgaaaa agagggcaca tgcgaaagcg ggccagttaa aacacagcaa
     5401 tgcaattcga caacaccaca gggtggcatg tattccgtgc cgtcactttc acacttcgac
     5461 cgcatgatcc cggactgcat cgagcttgcc aggcaggcaa aatcaggcgg caaaaaggta
     5521 gtttccattt tctgtgagta cacgccaagg gagctgatac ttgccgcggg agcgataccc
     5581 gtgtgcgcat gcggaggcaa ccattcaatg gccgtggcat ccgaaaagga gctccctgcc
     5641 aacctctgcc cgcttatcaa atcatcttac ggctaccaca ccgaaaaggc caacccgata
     5701 tttaacgagt ctgatctaat cgtgtgcgaa acaacctgcg acggcaaaaa gaagatgtat
     5761 gagctgatgt caaaaacaaa gcccatgcac attcttgagc tgacccagaa accggatgaa
     5821 gaggcagcat tcgagcactg gctgctggaa gtaaaagcgc tcaaaaagag gcttgaagag
     5881 ctgaccggaa acgaaatcac cgaagagagg ctcagggcag caatcaggac aatgaacagg
     5941 gagaggacgc tcaggtcaag gatcgcagat tttggcggga aatgccttac cggaatggaa
     6001 gtgctccacg ccaaaagcat catctcctgc caggagagag acttcaaggc atacgaaaaa
     6061 atcatatcgg aagctgaaaa ctctggtgac aggtactcgc acaagccaag gatactcctg
     6121 acaggcgtgc cattaccgca ccaggcagag aaagtcctaa aactgattga ggaagcgggg
     6181 gcagtggttg tggcgcagga aaattgcacc ggcctcaagc caatcgttga tcctgtcagc
     6241 gaacaaggcg atctcctgga aaatattgcc agaaaatact tcaagctgcc gtgttcagtc
     6301 atgatgccaa acaaagacag gtttgggtta cttgacagcc tgattgaaaa attcaggccc
     6361 catggcgtca tcgacctggt atggcaagct tgtcacacat acaacatcga atcagtactg
     6421 gtcaaaaaac atgtccagga aaaatggagg ctccctttcc ttaaaatcga gactgactat
     6481 tcaccttcgg acacggaaca gctaaggatc aggattgagg ccttcctgtc ggttgcatcc
     6541 caaaataact taaaaacgtc acactaaaaa agtaatattt gaaatttgaa tggccgtttg
     6601 aaaaagtatg ggaattatag gcccgaggac tgactatagt gtcagcgcaa gaagaggtat
     6661 tttttcatct tcatccatgg tttgcgcaag gccgacagtt atacaggttt ttagcttatt
     6721 gtttttgaga accaccagct catagccctc cgggaaatct ttgaggaaag gtcctgttac
     6781 ccccttgcaa aactgcggcg gtggctcgga gccaaggctc cactggtaaa gggccgttga
     6841 aagctgtctt ttgtattggt caaaaatgta tatagtcctc tcgccaaaat caggccaggt
     6901 aatgaccagt atgatatttc cacaatggcc gaatgccctt attgactctt ttgggttgcc
     6961 ctctatcatt tcgggatcgg caggaacacc gcctttatca atctggatgg ctttgccggt
     7021 gacccatttc ccatcaacaa actcccatgg caaaaggaca gtgcccccag cataagaata
     7081 aagccttgaa ggcatttggg gttctccatc cttcctgctg gtccagccag attcaaagcg
     7141 gtcataaatg cccctccctg gccaaacaag cacatgtttc tggagctggt tgccctcccc
     7201 atcaaaaaca aaagcagtct ccgttctctt gtccatttga ataaattata gcgccttgtg
     7261 ggattttgcc aagacctatt ttcccagata tgcctttttg gaggagagaa gtttttctat
     7321 agcccaattt ttctgtccaa aaacctgcca gaaaagtctt gatgtgtcct gaagatcatt
     7381 gtatgtaaga ttggtataat cttcgcggtc atatttttgc catgccggga aatacccata
     7441 ccagagatca ccattgtccc ttatccagct atcacaggtt tcctcaagac cggttatcag
     7501 tttcaatgca agctgtttga cggactcatc ttcatttaaa agatagagtt ccagcaagta
     7561 attgatttcg cagagcgtat gatttagtga agcatgaacc gggatttttg gtgttccctc
     7621 aaaccagata taatcttcaa acaaaaaacc atttccaaat tttattgtgt gctcgggtgc
     7681 aagcctctcg taggtttttg ctagcatcga tgctttttca aatgcaccct tgcaatcaaa
     7741 gatctgggca caatttatca agaacctgga acaatcagtt gaaaacctgg tgtccagata
     7801 gtctgaacca agcccgtacc atgattcaaa aagctcggaa atcacgggca ttttcttata
     7861 accaacatcg ggcgttgttt ctacagcaat tttcattgtt gtcaaaagca tgtctatcag
     7921 aaggcgattt ttctgctggc aataccactt gagtatactg ttcgaggtgt agaaaccagg
     7981 gttggggtaa aaagaatctt caacacaacc atcgtaatca ccaatctggg ttatgaacca
     8041 gaaaccatcc tgtctcagga tttttactct tccaagatca ctggcaagca aagcctcatt
     8101 gctcctctgg tttgtcatgt catgaaggag ttgtttggag ataataacaa aaccgtcttc
     8161 ccattcatcc tctgttttgt caaggtttat ttccaattgc attgaattat cgctagatga
     8221 gaagaatatg ccacttggcg gggcaattct ttttatcatt ccaacatctt gtttcttgta
     8281 gcgaccattg gaacaaataa agattgatct tttgtctttg ccataatcaa caaggcaagg
     8341 tccttctggg accctgattg tcccagagct aaaatttggc acgtactcgg attttatggg
     8401 atcgcctgca taattggagc gggactcata tgtatgggtc cagtatataa aacattttgg
     8461 gccctcgata ttagcgctga gcaaaatctt tgtttttggg gagtattgcg gctggtaaaa
     8521 ccagaaaaca acatctccat tgaggagtgt ctgcacagtg accactttat tcagatcatc
     8581 caatgtatat gaatcttctt ttataaaacg ctcaaaggag cctgagctca ttattttagg
     8641 gttggtctgg gtactatcaa cactgattga aaaactaacc agtggctcat caacaatgct
     8701 ttcacccttg gccagggtgc aaccggccaa caaaaccgcg agcaaaaaaa ttgccactct
     8761 tttcacggca gtgcgacacc agcccctttg tcctttgcaa aaacggacga tatcctgttg
     8821 ccccttgtgg ggcgcaatct tccaagaaac gcccacaaga gaccaagcag cgtgattacc
     8881 atgccaagcc atacaaacaa catgcagtat ttcaccataa cagtcatcgt aaatcttact
     8941 gtatttctat cagagtttct caggtcgaca agtttgaatg taactgtgtg atttcctatt
     9001 gcgccaatcg acaggaagta tgacttacca tcaatttctg caatggaccc aggagccata
     9061 ttgtctttgc tgaatgtgat ttcttttgct gaatccggcc catctgaaac cagggccttg
     9121 atgacatcct cattgtattc caggagtttg acggtgtatc cgccctgcaa tacagaactc
     9181 cctcttccaa caataaattc catgtcttcc ctgatatttg aaacatagac cattacatct
     9241 ctcaggaacg acctgtggat aaatggtttt gagaagtaga cctgtgacct tgtgtcccac
     9301 ttggcaccta ttttacctct tatttcagag tccttgtggg taagaacata catggtctca
     9361 aacgactgcc cgtcgctttt cacgtattcc agcccgacgc caaaatcaag gcattccttg
     9421 ttttgtgacg acattcccag ggaaaccgat tcctgcccaa aaaatatcga gcttcccact
     9481 acacccatca gcaggaggca aacgcccatg tgcgaaacaa aagcgcccca tttttggact
     9541 ctgtatttca acatggcctg gaagtttgaa accagcatta ttgaggcaaa tgcaccggta
     9601 atcatgaaaa gaggatcggc cattccagct tcaacggcca gcacgacaaa aacaagtcca
     9661 aaaaatagtg gcgcaataag ttgtcttgcc agatatttta tgtttgtttt tctccacgcc
     9721 atcaaagggg taagtgcacc gccaacacac attattatgg ctactgggat tgatattgcc
     9781 tgataaaatg attgtgagat gaccgcgggt gaaccaaaaa tctgggtcac aaccggcaac
     9841 agggtcgcgg caagtatgaa cacggcataa acagacagca ctatgttgcc aatgctcatc
     9901 atgaaatatc ttgaggtgta atggctctca aggccatggg gaacaaactt ccacgaacgg
     9961 aagcataaaa ataccgacaa tccgatagaa gccagaagca tgaccagata caaaaaaagc
    10021 atcgacgagc cagcaaaaga gtgaacagac acatccgaaa gcacgcctga tctggtcaaa
    10081 aaaactgcca ttacgacaag gcagaaagag gagagcacag cgacaacaac cgatctggcg
    10141 ttgccattgc cattctcat
//
LOCUS       DAOSRI010000024        10036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6517_length_10036_cov_3.522994, whole genome shotgun sequence.
ACCESSION   DAOSRI010000024 DAOSRI010000000
VERSION     DAOSRI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_10036_cov_3.522994"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /locus_tag="PK717_01330"
     CDS             complement(<1..2072)
                     /locus_tag="PK717_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46343.1"
                     /translation="MKKFPAFVLALLIAFSTIRGAASASTCPVSFYQIVIEPMISGAV
                     ACYTVVFQNQCTLPKGETIHLILPKGAEIYFGYGRNISLDGRPDRFMGQDNDLEIQLT
                     SDLPIGRHYLKVCNVKNPSDPGPHVIILTTSTERVMSFEFSFNQSSVSPPVVTVNPDY
                     VNACAEYTIRFRSASASGKGCGCHRETTISVMFPSGIEIGKIDFGGVYINGAAIPSSY
                     MRTYNSTIDVIVTFPINAGDLIEIRITTKAGIKNPSKPGWYTLKLYTSADQVVVTSEP
                     YYIRPSTVTRATVTLSNPYTCSVSAFQVRFRTGPYGSLLASSTITLMLPKVFLVPREI
                     EEGNIAVNSSKVSGKTYSSSWQEGPSITIRIPDNIGPETDVIIDVLPGAGIVLPEVDS
                     SYSIHVETSSEPYPVPSFDFYVQHSKLTAVSYSVEPLFVTLESKHSVTFVTGGCGDLE
                     PGLDTISVQFSKDFIMPQSMLCQTITINGVQIKNIPFMAGNTVTISPPERIPGGSEVT
                     INFPIECGIKNPGKPGTMHKVTVWTSRERAQVISNPVMFATTKLSNVFCELDKRLVGK
                     NTGAKVSFTLGKAGAIRNGTVVKIGFEDGFDFTGQVFIRNFVFDGAACRKATWANPVL
                     TLVCPKDYNPGDLVTINFDEEAGLKTPLKPGKYKIWVSTETEPDQIESDGLLVVDVPT
                     VSIQTTPKS"
     gene            complement(2077..2364)
                     /locus_tag="PK717_01335"
     CDS             complement(2077..2364)
                     /locus_tag="PK717_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46344.1"
                     /translation="MVPFGKLADILGFRVLIKDNDVIVADKFSSKTVVLFIGSDKIKI
                     RGEGNFEAQLSGKPIIKENILFVPLDFFDKGLGLQTIREENKITVLFCPRV"
     gene            complement(2331..4028)
                     /locus_tag="PK717_01340"
     CDS             complement(2331..4028)
                     /locus_tag="PK717_01340"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46345.1"
                     /translation="MKKFLSVIFCLALVTPGIALGVSSVSVVVTPDTATLNAQYEVQI
                     SLESPCPENAMVAMKFPEAVRLPDKIIGGLVAVSELPVQEVSVSDRTVSFKLPGPLPD
                     QKELSICIPAPCGLINPFKAGEYTLEITIDEESFRRNFQIKPILEQAPTVAVKPDKVG
                     YKVGITIQINTPDFFSVVEGDAIDITFPPEFELPESIDPQYVFISGSKIAAGSIDASN
                     IKLVCSQSVQKGEPVVISFEPSFGIKSPVWAGYFALGISIPGKMEEILSQPFQIQALS
                     PTLTLSVKPEIDDGAWYSVKPKIEITSKAKREIYYYWDQDKKNLYKDPFEAEEGIHML
                     SYVGKVIDGGYEAVLSKVFKVDLTPPVFFPTPTAFNAEKVELVYRVKDVSPCESGISK
                     DAAEALGDNTFKIDLALKPGPNDFVFWAVDSCQRISELRRTIILDVTPPTLIVEKPKP
                     LQTVCGSIIEVVGKTEPDCSIAVNGSPADVSEKGEFKAAVSPGSEGPFDITVVAIDPA
                     GNTTKKVIPVSYILNAKIGLEIGKVEFAYADSKGELEAVPYDDGVLPWCHSESSLIF"
     gene            complement(4025..6253)
                     /locus_tag="PK717_01345"
     CDS             complement(4025..6253)
                     /locus_tag="PK717_01345"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOR46346.1"
                     /translation="MKKHLKENEILEIASGAIGPVKKAMAGVHIKSCKECAKKIEDEI
                     AFREKVAEKLDKVASATTHSRMMDVRVLREIEVISKSKAPQRHPKWAVAVSLVCSVLL
                     FVYGFFFYAPVFGAAKETPAQAIVSGSFYPVIGQAGQLVPKHLDRMGNTYEKMAFFNI
                     LCDKYGVCEADRIKLVYQSGFDYKNVFVSSMLGMALDLNSEDVLNLISNGKTPGQVLS
                     KLGVPFTSQLSAAGEFQNQVNVTKADLATRDTISFDVLKVGKKITSPFPVNIPEGGKD
                     GHYIVRMDKNGKIISASPSKENAIWSGMIESAAFEKNEFVIKLDDGTLATVKISYETT
                     LKRYDDPILPNQLSAGQIVKAQGILSGKTLLAIIVDVREQDQKVSFKGLVADLDKSNV
                     AINGFGSALYLNPKTIYKGKPSIGETVEVEAIGNDITGYKVISMVVIPKPPQEKPVEK
                     ITVKSGIVVNVTTDEGGRRIFLLSDGTLVYYSRNTVFEGEKLSTGTVVAVKGVMDSEK
                     QMDARTVNVKNQAVTQTFSIAGELVDIRCITSCNWVKAEGVSEITVKGVTSPFLIYPE
                     TVGHSVVTPSRKGQIVILEGRTIAGVMLVDRAQVTDPTTQIIRTGIIVKMDQKEFMLD
                     DGSIFTLKSYTSKADGAAIGSTVSVTCMESDGSLIALKVELKEAEPEITSWLTVVSFE
                     NGELVLEDGTRFLVNGDTKIWIGIAKEKGTPDMLVQGAKVACTYRKGQNNIVLEILVQ
                     KQ"
     gene            complement(6256..6825)
                     /locus_tag="PK717_01350"
     CDS             complement(6256..6825)
                     /locus_tag="PK717_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOR46347.1"
                     /translation="MSQIDRKTEKKLILAAQKGSSDAFEQLYNEFRIELFKSAMLIMR
                     SSADAEDAVAETFIKAFKNIDRFDTRYPLRPWLHQILSNECSNIYKKSRGDVVQLDDF
                     STDWKENVVDKSNVEMDESMILDEEAQKVRNMIERLSYEHRQVVVLHYFQNHDIQAIA
                     VMLGVPVGTVKSRLFHARKQLLKICGQEN"
     gene            6972..8510
                     /locus_tag="PK717_01355"
     CDS             6972..8510
                     /locus_tag="PK717_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46348.1"
                     /translation="MKAKLAATVLFVAVLVVAPSQGLSEWPNPKGSMQRDLSSTAVHP
                     PFEIDWEEAIKPPSFLVCGNDRIIAVSVDSMSIFSISTFELVRTIQVQAAGEPCINDS
                     ILVIPSADGIHAYNLGNGSNAWDYPTGGIRYITNSPDGFLVSYEKTIICLDPKGNATF
                     RVELEAEILSCPVFCGNDKFVVPCKNAVIVLDFQTRQVIMNKPINDQVIYCSSPNSST
                     AVFVLSKGGSIAISVPSGETLWTNPEPRGSNIQIACDKSHVVLYGSSSGATCFRADGR
                     VLWKWSKHEVQSIVNAGRVLLASCQDDTLQVIQMATGTALNPVALPGKPTGTMAVDRS
                     FIYLSCGPKLIRLATSEYLAILGFTGNFDFGTIRPNTSAQKQIGVTNLSKKDQVVKMT
                     IGNKFCSMSPSIFSLTPGESKSVTIIIKPYDVLKLEEKIAIIADTSMYRYVLGAMLTV
                     DRLEGDANLDCVVDALDLIVLAKSLGLTSKSPLFDNKLDLDENGIIDLNDISIVERNF
                     GRTC"
     gene            complement(8901..>10036)
                     /locus_tag="PK717_01360"
     CDS             complement(8901..>10036)
                     /locus_tag="PK717_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46349.1"
                     /translation="GTLNITVREYGSHAPAAQAELKIEGAGLSIPSRRCNDKGQTTVS
                     VTPTETGIIRVKANLAGYADGEGYIVVGVDVQPPSIDQLEPIDYTVVNTNTVAVKGVT
                     KPGSTVTINGQKVGVDIKGAFQSNVTLKEGSNVIVVKATSPSGASTTRYLTIIKDTEK
                     PMLLVNDPTTIIHDATTYELTGRVEPGAKVTVNGQPATVVFDVWTATVNLVKGTNTVN
                     ISATDAAGNPNQTTIQLNNYTMQEVEFVVGNTVAYVNGTATTMPVAPTLSASNSVMVP
                     VDFLANAFGGTAPVAGTTATMTIGTITVDITVGSTNVNINGQTVTLPDAPVLNGTTIM
                     VPAEYLMLMVQNEIVKANPAKEIPLDIQWNDAARRLTITRYSL"
BASE COUNT         2543 a         2353 c         2272 g         2868 t
ORIGIN      
        1 gactttgggg ttgtctggat tgaaacggtc gggacatcca caacaagtag cccatcagac
       61 tctatctggt ccggctctgt ttctgtagaa acccatatct tgtattttcc gggtttgagt
      121 ggtgttttca aaccggcctc ttcatcgaaa tttattgtga cgagatcacc tggattgtag
      181 tcttttgggc acaccagcgt aagcacagga tttgcccatg tggctttcct acaggcggca
      241 ccatcaaaaa caaaatttct tatgaaaacc tgcccggtaa aatcaaaccc gtcctcaaaa
      301 ccgatcttta caacagtgcc atttcttatt gcgcccgctt ttccaagcgt gaaagagacc
      361 tttgcgccag tatttttccc gacaagacgc ttgtccagct cgcaaaaaac atttgaaagc
      421 tttgttgtgg caaacattac cggattggag ataacctgag ctctttcccg ggatgtccag
      481 acggtcactt tgtgcatcgt tccaggtttg ccaggattct ttattccaca ttcaatgggg
      541 aagttgattg tgacctccga gccaccggga atgcgctccg gtggtgagat tgtcacagtg
      601 ttgccggcca taaacgggat gtttttgatt tgcacgccgt ttatggtgat tgtctggcac
      661 aacatcgact gtggcatgat aaaatctttt gaaaattgca ctgaaattgt gtccagcccc
      721 ggttcgagat caccacaacc accagtcaca aaagtaacgc tgtgctttga ctcgagcgtt
      781 acaaacagtg gttctaccga ataagaaacg gcggtaagtt ttgaatgctg gacgtaaaaa
      841 tcaaaggatg gcacaggata aggctccgat gaagtttcaa catgaataga ataggaggaa
      901 tcgacctcag gcaacacaat gccagcaccg ggcaaaacat caattatgac atctgtctcc
      961 ggaccaatat tatccggaat tctgatggtt attgaaggcc cctcctgcca gcttgatgaa
     1021 tatgtcttgc cactgacttt tgaggaattg acggctatat taccttcctc aatctctctt
     1081 ggcaccagaa acactttggg aagcatgagg gttatggtcg acgaggcaag cagtgaaccg
     1141 taagggccgg tcctgaaacg cacctggaaa gcagaaacag agcaggtgta cggatttgaa
     1201 agtgtgacag tggccctcgt tactgttgat ggcctgatgt aataaggttc gcttgtgaca
     1261 acaacctggt cagcactcgt gtaaagcttg agcgtgtacc agcctggctt tgatggattt
     1321 tttatgcctg cctttgtggt tattcttatc tctatcagat caccagcatt gattgggaaa
     1381 gtgacaataa catcaattgt gctgttatag gtgcgcatgt atgacgatgg gatggccgca
     1441 ccatttatgt agacaccgcc aaaatcgatt ttccctattt cgatgcctga agggaacatt
     1501 acagatattg ttgtttccct atggcaacca caacctttgc ccgatgccga ggcagacctg
     1561 aacctgattg tatattctgc gcaagcattc acataatcag gattgacggt cacaactggt
     1621 ggcgaaaccg agctctggtt gaaagaaaac tcgaaagaca tgactctttc agtggacgtg
     1681 gtcaaaatta tgacgtgggg gccaggatcg gaggggtttt tgacattgca gacctttaga
     1741 tagtgccggc caattggcaa atcagaggta agctgaatct caagatcgtt gtcttgcccc
     1801 atgaacctgt ccgggcgccc atcaaggctg atgtttctgc catacccaaa ataaatctcg
     1861 gcgccttttg gcaaaatcag atgaattgtc tcaccttttg gcagagtgca ctgattctgg
     1921 aaaaccacgg tatagcacgc cacggcacca gaaatcattg gttctatcac aatctgatag
     1981 aacgatacgg ggcaagtgga agcacttgct gcccctctga ttgtggagaa agcaatcaga
     2041 agggccaaaa caaaggccgg aaactttttc atggttttat actctcgggc agaaaagcac
     2101 cgttatttta ttttcttccc tgattgtctg cagcccaagt cctttgtcaa aaaaatccag
     2161 gggaacaaaa agaatatttt cttttatgat tggttttccg gagagttggg cctcaaaatt
     2221 gccttcaccc cttattttta ttttgtctga accaataaag agcaccacag ttttgctcga
     2281 gaatttgtcg gcaacaatta catcgttatc ctttatcaaa accctgaaac ctaaaatatc
     2341 agcgagcttt ccgaatggca ccatggcaat acaccatcgt catatggcac cgcttcaagc
     2401 tcaccctttg aatcggcata tgcaaactca accttgccaa tctcgagccc tatttttgca
     2461 ttgagtatat atgagaccgg aatgaccttt tttgttgtgt tgccagctgg atcaattgct
     2521 acaaccgtta tatcgaaagg cccttcggag ccaggggaaa ctgctgcctt gaattcacct
     2581 ttttctgata catctgccgg cgacccattg actgctattg aacagtctgg ctctgttttc
     2641 cctacaacct cgattatgga cccacaaacc gtctggagcg gttttggctt ctcaacgata
     2701 agggtgggtg gtgtcacatc aaggatgatc gtgcgcctta gctctgaaat tctctggcag
     2761 gagtccacag cccagaaaac aaaatcgttt ggccctggct tgagtgcaag atctatcttg
     2821 aatgtgttgt cgccaagggc ctctgcggca tccttgctaa ttccggactc acacggagaa
     2881 acatctttta ccctatagac aagctcgact ttttcggcat taaaagctgt tggcgtcggg
     2941 aaaaacaccg gaggagtaag gtccacttta aagacctttg acaaaacggc ctcatacccg
     3001 ccatcaatta ctttcccgac atagctgagc atgtggatgc cctcttcggc ttcaaaagga
     3061 tctttgtaaa ggttcttttt gtcttggtcc caatagtaat atatttccct ctttgctttt
     3121 gatgttatct ctatttttgg ttttacagaa taccaagcgc catcatctat ttccggtttc
     3181 actgacaatg tgagcgttgg agaaagggcc tgtatttgaa aaggctggga gagtatctct
     3241 tccatttttc caggaatgga aattccaagc gcaaaatacc cggcccaaac cggagatttt
     3301 atcccaaaag agggctcaaa agatattaca acaggctccc ctttctgtac tgattgggaa
     3361 cagacaagct ttatgttgct ggcatcaatg gaacctgcgg caatctttga accgctgata
     3421 aaaacgtact ggggatcaat tgattctggc aattcaaatt ctggcgggaa ggttatgtca
     3481 atcgcatcac cttcaacaac actgaagaag tcaggggtgt ttatctggat tgttatccca
     3541 accttgtaac caactttatc tggctttaca gccaccgttg gggcctgctc aaggattggt
     3601 tttatctgga aattccttct gaaagattcc tcatcaatcg tgatttccag tgtgtattct
     3661 ccggctttga acgggtttat caacccacac ggtgccggta tgcaaattga gagttctttc
     3721 tggtcgggaa gcggcccggg gagtttaaaa ctgactgtac ggtcactgac tgaaacttcc
     3781 tgcacaggta attctgaaac agccacaagc cctccaatta tcttgtccgg caatctcact
     3841 gcttcgggga atttcattgc caccatggcg ttctcggggc atggggattc aagcgatatt
     3901 tgcacctcat actgggcgtt caacgtggcc gtatcgggtg ttacaaccac agaaactgaa
     3961 gaaaccccca gagctattcc aggggtgacc agcgcaagac aaaatatcac ggaaagaaat
     4021 tttttcattg tttttggacc aatatctcca gtactatgtt attttgaccc ttacggtaag
     4081 tacatgccac ctttgcaccc tggacaagca tgtccggcgt tcctttttct ttggcaatgc
     4141 cgatccagat tttggtgtcg ccgtttacaa ggaaccttgt tccatcttcc agaacaagct
     4201 cgccattttc aaaagatacg acggtaagcc atgaagttat ttctggttca gcctctttca
     4261 actcgacttt cagggcaatc agggaaccat ctgactccat gcaggttacg ctgacagttg
     4321 agccgattgc agcgccatct gccttggatg tgtatgattt cagtgtaaag attgacccat
     4381 catccagcat aaattccttc tggtccattt tgacaatgat gccggtacgg ataatctgtg
     4441 ttgttggatc ggtcacctgg gccctgtcaa caagcattac tcctgcgatt gttctcccct
     4501 caagaatcac gatctgccct tttcttgaag gagtcacaac actatgaccc actgtttctg
     4561 gatagatgag gaatggtgac gtaacaccct tcacagttat ctcagagaca ccctcggctt
     4621 tgacccagtt gcacgaggtg atgcacctga tgtcaacgag ttcaccggca atggagaagg
     4681 tttgggtcac tgcctggttc tttacgttaa cagtcctggc gtccatctgc ttttcagaat
     4741 ccatgacgcc cttgaccgca acgactgtcc cggttgaaag cttttcgcct tcaaaaacag
     4801 tgtttctgct atagtagaca agtgtaccat cagagagaag gaatattctt ctcccacctt
     4861 catcagttgt cacattcaca acaatgcctg atttgacagt gatcttttcg actggttttt
     4921 cctgtggagg cttcggtata accaccatgg agataacctt gtaacctgtt atgtcatttc
     4981 cgatggcctc gacttcaact gtttcaccaa tggatggttt tcccttatag atggtttttg
     5041 ggttcaggta gagcgccgaa ccaaaaccat ttattgcgac attgcttttg tccagatcag
     5101 caacaaggcc cttgaaactg accttctggt cctgttccct gacatcaaca atgattgcca
     5161 gaagggtctt accagagagt attccctgcg cttttacaat ctgtccggca gaaagctggt
     5221 tcggcaggat tgggtcatca tatctcttga gggtagtctc gtaactgatt ttaacggttg
     5281 ccagagtgcc atcatcaagc tttattacaa actcattctt ttcaaaagct gctgattcta
     5341 tcattccaga ccagatggca ttctctttgg atggcgaggc cgagattatt ttgccgtttt
     5401 tgtccatcct gacaatatag tgtccgtctt ttccaccctc tggaatattg accgggaatg
     5461 gcgaggttat ctttttgccg accttcagaa catcaaagct tatagtgtct cttgtggcaa
     5521 gatcagcctt tgttacattg acctggttct ggaattcccc agctgcagaa agctggctgg
     5581 tgaaaggaac tccaagcttc gaaagcacct gacccggggt tttaccgttg gagatgaggt
     5641 ttagcacgtc ctccgagttg agatccagcg ccattccaag cattgaagaa acaaaaacat
     5701 ttttgtaatc aaaaccactc tggtaaacga gctttattct gtcggcctca caaacaccat
     5761 acttgtcaca aagaatgttg aaaaaggcca ttttttcgta cgtattgccc attctgtcca
     5821 aatgcttcgg gacaagctgt ccagcttggc caatgaccgg atagaagctt cctgaaacaa
     5881 tcgcctgggc tggggtttct ttcgctgcac caaaaacagg ggcatagaag aagaaaccat
     5941 aaacaaaaag cagaaccgaa caaaccagcg acacggcgac agcccatttc gggtgtcttt
     6001 gcggagcctt tgattttgag attacttcta tttctcttaa taccctcaca tccatcattc
     6061 tggaatgggt cgtggccgag gccaccttgt caagtttctc agcgaccttt tccctgaaag
     6121 ctatttcatc ttcaatcttt tttgcacatt ccttgcatga ttttatatga acccctgcca
     6181 tagctttctt taccgggccg atggccccag aggcgatttc taggatttcg ttttctttta
     6241 ggtgcttttt catttttaat tctcctggcc gcatatcttg agcagttgtt tgcgtgcgtg
     6301 gaacaacctt gacttcacag tcccgaccgg aacacccagc attacagcga ttgcttgtat
     6361 gtcgtggttc tggaagtaat gtagcaccac gacctgccgg tgttcgtacg agagccgttc
     6421 aatcatgttc ctgacctttt gcgcttcctc atccaggatc atggattcgt ccatctcaac
     6481 gtttgacttg tcaacaacat tttctttcca atcagttgag aaatcatcaa gctgaaccac
     6541 atcccctctt gatttcttgt agatgttgct gcactcgttt gagagaattt ggtgcagcca
     6601 gggtctcaag gggtacctgg tgtcgaatct gtcgatgttc ttgaaggcct tgataaaggt
     6661 ctccgcaacg gcgtcttcgg catctgcgct gcttctcatt atcagcatcg ctgacttgaa
     6721 aagctcgatc cggaattcgt tgtaaagctg ctcgaaggca tcagaggagc ccttttgagc
     6781 cgccagaata agtttcttct ccgttttgcg gtcaatctgg ctcattctgt cttcaccgca
     6841 aatacgatct ttgtgtttct ttggttcatg tttgacacat ttaagatttt aaactttgtt
     6901 tgccatcttg ccaagattgt gaagttgttt gtgattgact ttttgcgcaa aatgcaggta
     6961 gaataaggga aatgaaggct aaactcgccg ccaccgtttt gtttgttgcg gtcctggtgg
     7021 tggccccctc acaaggtctt tcagaatggc ctaatccaaa gggaagcatg caaagggact
     7081 tgagttcgac tgctgtacat cccccttttg agatagattg ggaggaggcc atcaaaccgc
     7141 catccttttt ggtttgtggc aatgacagaa tcatagcagt ttctgtagat agcatgtcca
     7201 tattctccat atcaaccttt gagttggtaa ggaccataca ggtgcaagcc gctggagagc
     7261 catgtattaa tgacagcatt ttggttatcc caagtgctga tggcatccac gcatacaacc
     7321 ttgggaatgg ttccaacgcc tgggattacc caactggggg aattaggtat ataacaaact
     7381 cacctgacgg ttttctggtt tcttacgaaa aaacaataat ttgcctggac ccaaaaggga
     7441 atgcaacctt tagggtggag ctcgaggcag aaatattgtc ttgtcctgtc ttttgcggaa
     7501 acgataaatt tgtcgtgcca tgtaagaatg ccgttattgt gctagatttt caaacacggc
     7561 aagtgatcat gaacaagcca atcaatgatc aggttattta ctgctcatca ccaaactctt
     7621 ccactgcggt atttgtgctt tcaaaaggtg gtagtatagc catttcagtg ccgtctggag
     7681 aaacgctctg gacgaacccg gaacccaggg gttccaacat tcagatcgca tgtgacaaga
     7741 gccacgttgt gctctacggt tcttcctctg gagcaacatg cttcagggca gatggaagag
     7801 ttctgtggaa atggtcaaaa catgaagtcc aatcaattgt gaacgccgga agggtcctcc
     7861 tggcttcgtg ccaagacgat acgctccagg tgattcaaat ggcaacagga acagccctca
     7921 atcctgtcgc actgcccgga aaacccaccg gaacaatggc agttgaccgc agttttatat
     7981 atttgtcctg tggacccaag ttgattaggc tcgcaacaag cgaatacctt gcaatactgg
     8041 gttttacagg caatttcgat tttggaacca tcagaccaaa cacctccgcc caaaaacaaa
     8101 tcggggtgac caacctaagt aaaaaagatc aggtagtgaa gatgaccatc ggaaacaaat
     8161 tttgttctat gtctccttca atctttagcc ttacaccagg cgaatcaaaa agtgttacca
     8221 taatcataaa gccttacgat gttctcaaac ttgaggaaaa gatcgccatt atcgccgata
     8281 catcaatgta cagatatgtt cttggggcca tgctcacagt tgacagacta gagggtgatg
     8341 caaaccttga ctgtgttgtg gatgcacttg atctaatagt tctggcaaaa tcgctcgggc
     8401 tcacatcaaa atcacccttg tttgataaca agctcgatct ggacgaaaac ggcattattg
     8461 acctcaacga tatatcgata gtcgaaagaa acttcggcag aacctgctaa ttctataagg
     8521 ttgcctatta aaaaacagac tgtccagcat tcttttttgt ttcattgcca ataacggtct
     8581 ttaaaagctt aaggaccatt ccatccggca ttcggtcaat aaaaatcatt caaagaataa
     8641 agaatctccc agttcaaaat catatctgag ggtctattca ttcataaata gctattcagt
     8701 ttggctttag gtcgtgcttg tttaaagtag ccgcggccag aattgcacat attgccatat
     8761 tgttaccgca agcttcccca ttcaaaagta gaagctgatg gaactcctca tcaatggttc
     8821 ttattgatga gccccacata agccaataaa aaagccccgg tttttggccg gggctttttg
     8881 tttttattgg taccttaata ctacagtgag tacctcgtga tggtaagcct tctggctgca
     8941 tcgttccact ggatgtcaag cggaatttcc ttggccggat tagctttcac gatctcgttc
     9001 tggaccatca gcatcaagta ctcagccgga accatgattg tagttccatt gagcactggt
     9061 gcatctggca gcgtcacagt ctgaccattg atgttgacat ttgttgaacc gacggtgata
     9121 tcaacagtga ttgttccgat tgtcatagtg gcagtcgtgc cagcaaccgg tgcagttcca
     9181 ccgaatgcat tggcaaggaa atcaactgga accatgactg agttggaggc tgagagcgtc
     9241 ggggcaactg gcattgttgt cgctgtgcca ttgacatagg cgaccgtatt gccaacaaca
     9301 aactcgactt cctgcattgt gtagttgttg agctgtattg tggtctggtt cgggttgcct
     9361 gcagcatcag ttgcagaaat gttgactgta ttagtgccct tgacaagatt gactgttgct
     9421 gtccagacat caaacactac tgttgctggc tggccgttta cggtgacctt tgcgcctggt
     9481 tcgacacgtc cggtgagttc gtatgttgtt gcgtcgtgga tgatagttgt tggatcattg
     9541 accagcaaca ttggtttctc ggtatctttg atgattgtca ggtatctggt tgttgatgca
     9601 ccgcttggtg aggtggcttt gacgactata acgttggaac cttctttgag tgttacattg
     9661 ctctggaagg cacccttgat gtcaacgccg accttctggc cattgattgt aactgtagaa
     9721 cctggctttg tgacaccctt gactgcaaca gtattggtgt tgacgactgt gtaatcaatt
     9781 ggttcaagct ggtcaattga tggtggctgt acatcgacac ctaccactat gtagccttct
     9841 ccgtcagcat aaccggcgag gttggctttg actctgatga taccagtctc ggttggtgta
     9901 actgaaactg tggtctggcc tttatcattg catcttctgg atgggataga gagtccagca
     9961 ccttcaatct tgagctcagc ctgtgcggct ggagcatgtg aaccatattc acgaacagtg
    10021 atgttgagtg ttcccg
//
LOCUS       DAOSRI010000025        10017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6532_length_10017_cov_3.857960, whole genome shotgun sequence.
ACCESSION   DAOSRI010000025 DAOSRI010000000
VERSION     DAOSRI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_10017_cov_3.857960"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK717_01365"
     CDS             <1..763
                     /locus_tag="PK717_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HOR46350.1"
                     /translation="NKISDALETRLPKTYKGRNVPFSDENGVYVWFWPDFTEYILGLR
                     RRPIDPISYAKVSEAQFYALLSGTIFWDPSYELQRAQLQWDHMPQEVWYWRHHWIFRN
                     LENRKLVRLVIEDDDKLTAKMMISRYIEWTIRLSFLAARKYSPRRSLLLEAHSQLQKT
                     EEIHQLITSAIESHSAEGALEKIEKAIMAIRLKMIEKKYLPENIGLDENGNTDCLAMS
                     EYFLSAMPGDFRKQASRVCPVDLLGSLEQRAKNTP"
     gene            863..1996
                     /locus_tag="PK717_01370"
     CDS             863..1996
                     /locus_tag="PK717_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46351.1"
                     /translation="MKLRTTIIGKNPINGVYIEDIYDLNSHSYYSIIADGDVLKICDK
                     KTRSLIFSELKLPGAAISSYYAEHKYYMICKSQKGNSLVCFSYDDTLRKLELHKKFEV
                     KTQSSQIVSELPGMDGVITQSEDKTILYLYTKGKLIKSIKYSKPTIWWHPNYYDHLQN
                     IGFDGQDLIFQTDSDHDGIFYEIREKIKEPSSIDNAFFQPTGWYERYVDLYDGINLWR
                     IETCLNGLGNMHIKGPLEIKNLKKGFNQILYPYEKGLCRVEISKTDSSKLVLKHIDDR
                     SMYPVVEFSRNWTIPAGYYLYGCKENDSYELKCYKFFWEDIENSDRNLAYKECDVEAV
                     DSREGIISGDYYNPNNDYSSSRDVSFIIFTKDGIYYIPDATNW"
     gene            complement(2111..3622)
                     /locus_tag="PK717_01375"
     CDS             complement(2111..3622)
                     /locus_tag="PK717_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46352.1"
                     /translation="MNHIKIIVLIAIMALCSCSTAQTAGLIDKKNNPSSSLPPPIGND
                     GLWSNGTQMIAVSGGDTEYLSTQTKWLLNKSWDAEQWMYGSWENAKLVDGKEASYAIN
                     KTVMPWLKLYERGAQWVYGCNPYYSWNNPPPTKLNAPDIIKPGKPLPPERTIQTLPSY
                     YRHFFEVSSKSSGFLTIWVSGEVDVYLDPPVGTRLVKNSTSKSYLGSYSGIDKESGNV
                     INLPLINPGAHCIYFVHRSDPYSNFFGMIFGLETFSLPGQKSACGWDITNGDCACAVS
                     NETAEYLVHNDSGNNPASSKWEVFGLPKGTEWVNGNWAPVTTIPWERADNDAWGVDVQ
                     GAPWRKLYDMGANWITHIPSGGVAGMFPPGNIGWNSCPKNITNIYRQAFTVDKHCTAE
                     LTVYTNDDVDIYIDPFISIPNPGQSTKLVLQSPTFGLSNPPHSNQPETSYYYIGRADK
                     QRRGMIVNLQIVLTPGTHTIYFVHRNSFDPKPGKTRDYYGLLYALSCKGSFCK"
     gene            complement(3783..5375)
                     /gene="pruA"
                     /locus_tag="PK717_01380"
     CDS             complement(3783..5375)
                     /gene="pruA"
                     /locus_tag="PK717_01380"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOR46353.1"
                     /translation="MRQQFVSEPYLLNLSNPEQKKMMQDAIDFVGKNLGKHYDIIIGG
                     RNIKTNDVIKSSNPSKHNEIVGTVSKGNSQLAEEALQTAHKTFMEYWRFTDPHERARY
                     LYRAAGIMRRRKLELSAWMVYEVGKSWIEAIADTAEAIDFLEYYGRLMENISVPYNIP
                     SIPGEDNQYWHIPLGVGVVIPPWNFPLAILTGMTTAAVVTGNTVVLKPASVTPIIGAK
                     FMEVMKEAGIPDGVINFLPGPGSEVGDYLVDHPLTRFISFTGSMDVGKRIYERASKVN
                     KGQIWLKRVVAEMGGKDCMVIDGNYSKPDFAAAETVSAAFGFQGQKCSACSRLIIVGD
                     QYDDIVGRIVEKAKAIKVGDTRDTSFGMGPVSSAQAFSTIKEYIEIGKTEGKLLVGGT
                     CDDSTGYFIQPTVFGDVSPTARIAQEEIFGPVLSISRAKSFDEALDYANGTIYGLTGA
                     LLSDSGKNLERARREFHVGNLYLNRKCTGALVGSQPFGGFNMSGTDSKAGAFDYLYLF
                     MQGKSCSERLDSDQFAKYLGIK"
     gene            5619..5798
                     /locus_tag="PK717_01385"
     CDS             5619..5798
                     /locus_tag="PK717_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOR46354.1"
                     /translation="MSFGAKEIIVILIVGLILFGSSKIPEFARSLRKGIEEFKKTGEE
                     LSKGIEAKDDGQAKK"
     gene            5810..6553
                     /gene="tatC"
                     /locus_tag="PK717_01390"
     CDS             5810..6553
                     /gene="tatC"
                     /locus_tag="PK717_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOR46355.1"
                     /translation="MNDKPKPVLEHIEELRKRLIYSLLFVLVFSVVCWIYSDQIIAWL
                     TGPVPFLDVPPIQLHFTDVTEAFGAKFMISIIGGIIMAFPFLIYHMCMYILPGMKKNE
                     RKAFFAYLPSMILLFIAGAAFAIWPIVPIARKFFVDYGGQNMVPIITVSSYIDFVTLL
                     TLGMGIVFLLPVVVMFITSIGLVSPYTLAKGRKGVWLGILIAAAAIAPNDGLSMLIIA
                     LPVVILFEISLFLSKIKWRRRQKIESEPA"
     gene            complement(6550..8727)
                     /locus_tag="PK717_01395"
     CDS             complement(6550..8727)
                     /locus_tag="PK717_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46356.1"
                     /translation="MKKISFLVALMFIVPSQAILPAAGDAKPIATAPFQVALKCSVGG
                     SYLSAYSPTFFDSGYLIGNASGKLVYIKSGMKKFELEIDGADIASSPLVYNASCFVGT
                     KAGTLALVDLLGKKIVWSFRAGNAIYGMPVLFDGKIIFGCSDGNLYCLNATDGSLVFR
                     FDSKSPIWGTPAIYSGSVIFGGDDNRLRRVSVPEGKEIWSFETGGWIETQPLIFGQKV
                     IASSLDHVLYCLNADDGSLLWRYIAEAPIRSNPVLVGSKCIFADESGILYAVDAETGR
                     QEWKKEGFGPVSWPLATDGQSIYFIDGNKTFWGIDPEGSVVWKRKLVHATRSSVLIEG
                     GRISYVTSDGQLFCWEECPYAEVLENRDLGTIVASDGTKRFPFTLLFGREDGRLPPPK
                     FEGMPFEAGSRCKSVSFIGGKTFSEGMRRGYKYTMIIDPSDPSFCDGKQKFGVAIEPG
                     CPVMEGNGQYSEPGISGWVLVFFTVDIVSSQKKCQTCEPCFDVTISPSDVPYFPRSSQ
                     GRFEVRINSTDGEDRYIRLDIDKPDWLKVDGQGGKYQENGTTSFSFSFNCMDLPGGSD
                     FSVPIKITCNTCPERNGGMSSWEDELSLDFVTDPRLRIQMEPGSSEALINSGKVLLDV
                     PAEVYKGRTLVPIRFIAETFGCKVDWNATEKKVEISRKGKVLKYWIGEDRAEFDNKVV
                     KIDVGPLIRNGRTLVPLRSVAESLGAIVRWISVAKSIIIDMEF"
     gene            8828..9352
                     /locus_tag="PK717_01400"
     CDS             8828..9352
                     /locus_tag="PK717_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46357.1"
                     /translation="MRKKLLSFVVLGVGAIIAQIVVALFYSGNSENLWQILGFGNLFV
                     LIACFMASFKNKLQILPLLGLIIGISSPVLSFIWMGRGFAGQAISYSLSYFCFSMHYI
                     LQREKPLRYFGWPLLLCSFLLWSFHIFLANAGSGLPPFDIIAFFFSLLMLLAASSILL
                     IIIFEKNTIQEDIS"
     gene            9349..9942
                     /locus_tag="PK717_01405"
     CDS             9349..9942
                     /locus_tag="PK717_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46358.1"
                     /translation="MNQILKSKFNAWGFFIVACVWVLMMICSLMFSILDINVDVLSPI
                     IRMLMVGTGMVMTFFFFSISRSEPGLAAVGLVLGIVGTIAQISPTESGFSEIVGFIIG
                     ISYIFFAFAILKQEAKAAGWLGWAMILSGIIKLSSIGMITGMMRGELGGIIIFSAITI
                     LFEIFVALAAIYYIIVGGNLFEGTGAPKTINAPENLN"
BASE COUNT         2899 a         2203 c         2069 g         2846 t
ORIGIN      
        1 aaacaagata agtgatgccc tagaaacaag attgccaaaa acttacaaag gcagaaatgt
       61 tccattttca gacgaaaatg gcgtatatgt atggttctgg ccagacttta cagaatatat
      121 tcttggcctc aggagaagac caatcgatcc aatctcctat gccaaagttt cagaggcgca
      181 gttttatgca ctgctttccg gcacaatctt ctgggatccc agctacgaac tccagagggc
      241 gcaactccag tgggaccaca tgccgcaaga agtatggtac tggagacacc actggatatt
      301 caggaacctc gaaaacagga aacttgtacg actcgtcatc gaagacgacg ataaactgac
      361 cgcaaaaatg atgatttcaa ggtatatcga gtggacgatc aggctgagtt ttctcgccgc
      421 aaggaaatat tcccccagaa gatctctgtt gcttgaagca cacagtcagc tccaaaaaac
      481 tgaggaaata catcaattga taacatcggc aatcgagagt cactcggccg aaggggcgct
      541 tgaaaaaata gaaaaggcca tcatggcaat aaggctgaag atgatcgaaa aaaaatactt
      601 gccagaaaat atcgggcttg atgagaacgg aaacacagat tgccttgcca tgtcagaata
      661 cttcctgtcg gcaatgcctg gcgatttcag gaaacaggct tcaagggtct gcccggttga
      721 tcttttgggg tcgctggagc aaagggcaaa aaatacgcca tgaaatagaa ccaaaataat
      781 ggggaggtgc caaatgagga ggtttttatc ttgtcttttt gtgttacttg tagctatatg
      841 ttgtagttgc tctcaacctc caatgaaact cagaacaaca attattggca agaatccaat
      901 caatggtgtt tatattgagg atatttatga tttaaattca cacagttact acagcataat
      961 cgcagatgga gatgtactaa agatatgcga caagaagaca aggtctctca ttttctcgga
     1021 attgaagctt cctggagcag ccatatcgag ctattatgca gaacataaat attatatgat
     1081 ttgcaaatct caaaaaggca actcgctggt ttgcttctca tatgatgaca cccttcgtaa
     1141 gctagaattg cacaaaaaat ttgaggttaa aacacaatcc tcacagattg taagtgaatt
     1201 gccaggtatg gatggtgtca ttacccaatc agaagataaa acaatcttgt atttatatac
     1261 gaaaggtaaa ttaataaaaa gcatcaaata ctccaaacct acaatctggt ggcatcccaa
     1321 ttactacgat catttgcaaa atattggttt cgatggacag gatttgattt ttcaaacaga
     1381 tagcgatcat gatggcattt tttatgaaat tagggaaaag atcaaagaac catcatcaat
     1441 tgataatgct ttctttcagc ctactggatg gtatgaacga tatgttgatc tttatgacgg
     1501 aataaatctc tggagaatcg aaacttgttt aaatggatta ggcaatatgc acattaaggg
     1561 ccctcttgaa attaaaaatt taaagaaagg attcaatcaa attctatacc cttatgaaaa
     1621 gggcctttgt agggtggaaa ttagtaaaac cgattccagt aagttagttt tgaagcatat
     1681 tgatgatagg agcatgtatc cggttgtcga attctcaaga aattggacca ttcctgcagg
     1741 ctattatctt tatggatgca aggaaaacga cagttatgaa ttgaaatgct ataagttttt
     1801 ctgggaagat attgaaaata gtgacagaaa tttagcctat aaagagtgcg atgtcgaggc
     1861 tgtcgattct agagagggta ttataagtgg cgactattat aacccgaata atgattattc
     1921 aagcagtaga gatgtttctt ttattatttt tactaaagat ggcatttatt acattcctga
     1981 tgccacaaac tggtgattca agattaattc ttggtgcaca aaaatataat tcctgtaatt
     2041 attttcgcaa ttccaaacta aaaccccctt ggttttccaa gggggtttat catttttgcc
     2101 actctatcgt ttatttgcag aaacttccct tgcatgacaa ggcatacagc aatccataat
     2161 aatcccttgt tttgcccggc ttcggatcaa agctgtttct gtgcacaaaa tagatcgtat
     2221 gtgtcccagg ggtcaagact atttgcaaat tgacaatcat tcccctcctc tgcttgtctg
     2281 ccctgccaat gtagtaatac gacgtttctg gctggtttga atgaggagga ttgctcaagc
     2341 caaaggtcgg tgactgaagc acaagcttgg ttgattgccc cggattggga atgctgatga
     2401 acgggtcaat gtatatgtcc acatcatcat ttgtgtagac cgtaagttca gcagtacagt
     2461 gtttgtcaac agtaaatgcc tgtctataga tattagttat gtttttcgga caactgttcc
     2521 agcctatgtt gccaggaggg aacatcccag ccacaccacc ggaaggaata tgggttatcc
     2581 agttagcgcc catatcataa agtttcctcc atggcgcccc ctgaacatca acaccccagg
     2641 catcattgtc tgctctctcc caagggatgg tagtgacagg tgcccagttg ccgtttaccc
     2701 actcggttcc ctttggtaat ccaaagactt cccacttgct gcttgcagga ttattgccag
     2761 aatcattatg gacaagatat tctgccgtct cattgctgac agcgcatgca caatctccat
     2821 ttgtgatgtc ccatccacat gcagattttt ggccaggcag ggagaatgtt tcaagcccaa
     2881 aaatcatccc aaagaaatta gaataagggt ctgacctgtg aacaaaatag atgcaatggg
     2941 caccagggtt tataagaggc agatttatca cattcccgga ttctttatca attcctgagt
     3001 agcttccaag gtagctcttg gaggttgaat tcttaacaag gcgggtccct actgggggat
     3061 caaggtagac atcaacttct ccagagaccc agatagtcaa aaaacctgag gatttactgc
     3121 tgacttcaaa aaagtgcctg taatagctgg gtaaagtttg aattgtgcgt tctggaggaa
     3181 gaggtttacc aggttttatt atgtcaggtg cattgagctt tgttggtgga ggattgttcc
     3241 aggaataata aggattgcac ccataaaccc attgagcacc cctttcataa agcttcaacc
     3301 atggcattac agtcttattg atggcatagc ttgcttcttt cccgtcgaca agctttgcgt
     3361 tctcccacga accatacatc cattgttcag catcccagct tttattgaga agccattttg
     3421 tctgagtcga aagatattca gtgtcaccac cagaaacagc gatcatctga gttccattgc
     3481 tccataatcc gtcattgcct attggaggag ggagagaaga agatggatta ttttttttat
     3541 cgatcaagcc tgccgtttga gcagttgagc aggaacataa agccatgatg gcgatcagaa
     3601 caataatttt gatatggttc atataaccac cttcccaatc ctttattgta taaaaactaa
     3661 tctaaaattt aatcatgtta aatcaagttg ttaaaaaggc gaaaataaag aaaaatatta
     3721 tcgaaatata aaaagccccc acttttggaa gtgggggctt ttaaaataat tattgtccct
     3781 tgctacttta ttccgaggta tttggcaaat tgatcagaat cgagcctctc cgagcaagac
     3841 ttgccctgca taaagaggta aagataatcg aacgcgccag cttttgaatc ggtgcctgac
     3901 atgttgaaac caccaaatgg ctgtgagccg acaagagctc cagtacattt cctattgaga
     3961 tacaggttgc caacatggaa ctctcttctg gccctttcca gatttttgcc agagtcagac
     4021 agaagggcac cagtgagccc gtagattgtt ccatttgcat aatcgagggc ttcatcaaag
     4081 gattttgccc tcgagatcga aagcactggt ccaaaaatct cttcctgggc aattcttgca
     4141 gttggactta catcgccaaa aacagttggc tggataaaat atccagtgct gtcatcacaa
     4201 gtaccaccaa caagcagctt gccttcagtt ttccctattt caatgtactc cttgattgta
     4261 gagaatgctt gcgctgagga aactggtccc ataccaaaag aagtatctct cgtatctcca
     4321 acctttattg cctttgcttt ttccacaatg cgaccaacaa tatcgtcata ctgatcgcca
     4381 actataataa gccttgagca ggcagagcac ttttgacctt ggaagccaaa tgctgcggag
     4441 actgtttcag cggctgcaaa atcaggtttg ctgtagttgc catctataac catgcagtct
     4501 ttgccaccca tctcggcaac gactctcttg agccaaattt ggcccttgtt gacctttgaa
     4561 gctctttcgt agatcctctt gcccacatcc atggacccag taaaactgat gaaacgggtg
     4621 agcgggtgat caacaaggta atctccaact tcgctgccag gtcccggaag gaaattgatg
     4681 acgccatccg gaatgccagc ctctttcatc acttccatga atttcgcacc aataattggg
     4741 gtgactgaag ctggtttcag gacaactgtg tttcctgtga caacggccgc agtagtcatt
     4801 ccagtcagaa ttgcaagcgg gaaattccac ggtggtatta caacaccaac gccaagcgga
     4861 atatgccagt attgattgtc ctcgccaggg attgaaggaa tattatatgg aacagagata
     4921 ttttccatca gtcttccata atattccagg aaatctattg cctctgcagt atcagcaatg
     4981 gcctcaatcc agctcttgcc aacttcataa accatccacg cagaaagctc cagtttcctt
     5041 ctcctcatga tgccggcggc tctgtaaagg tatctggcgc gttcatgcgg gtcggtaaaa
     5101 cgccaatact ccataaacgt cttgtgagcg gtctgtaaag cctcctcagc taactggctg
     5161 ttgcccttgc ttaccgttcc cacaatctca ttgtgctttg atggattgct tgatttaatc
     5221 acatcgttgg tcttgatgtt ccttccaccg attatgatgt cgtaatgctt gccaagattt
     5281 ttaccaacaa aatcaatggc atcttgcatc atttttttct gttcggggtt ggacaaatta
     5341 agaagatacg gttcagatac aaactgctgt ctcatgtggc tcctttcagg tgcttgttga
     5401 caaccaacat tatatatatg aaaaaaggtt aaatccagta ttgatgacta taaactcgtg
     5461 cgcaagcaca acaaatgaga aatcgcctga tacgagaatc gtgggtaaaa atacaaatcg
     5521 aagaagttgc tgacaaatag tccaaaaatc tcaaaatata agggaataat tcttgaaatg
     5581 gccttttccc acaatatgct ctcttggtga ggtgaaatat gagctttggt gccaaagaaa
     5641 ttatagtaat actgatcgtc ggattgatcc tttttgggtc aagcaaaata ccggaatttg
     5701 caagatcctt gcggaagggg attgaggaat tcaagaaaac cggcgaagaa ttatcaaaag
     5761 ggattgaagc aaaagacgac gggcaagcca aaaagtaggc gctcttttgt tgaatgacaa
     5821 gccaaaacct gtccttgagc acattgagga gctcaggaag aggctcatct actctttgct
     5881 ttttgtgctg gttttttcag ttgtctgctg gatatattca gaccaaataa ttgcatggct
     5941 gactggccca gtaccttttc tcgatgtccc accaatccag ctccatttta cagatgttac
     6001 agaagctttc ggcgccaaat ttatgatatc gattatcggc ggcatcataa tggctttccc
     6061 tttcctgata taccacatgt gcatgtatat tctccctgga atgaaaaaaa acgagcggaa
     6121 agcctttttt gcatatctcc cctcaatgat tttgctcttt attgctggcg cagcattcgc
     6181 catttggcca atagtcccca tcgcaagaaa gttctttgtc gattacggtg gccaaaacat
     6241 ggttccaatt attacggtat cttcttatat tgatttcgta acgttgctca cccttggaat
     6301 ggggattgtt tttctgcttc ctgtcgtcgt catgtttatc acatcgatag gccttgtttc
     6361 cccatataca ctggcaaaag gcagaaaagg ggtgtggcta gggattctga ttgctgctgc
     6421 tgccatcgca ccaaatgatg gattatcaat gttaataata gctttgccgg ttgtcattct
     6481 ttttgaaatt tcgctgtttc tttctaaaat caaatggaga agacgccaaa aaattgagtc
     6541 ggaaccggct taaaactcca tatcaataat tattgatttt gcaacagaga tccacctcac
     6601 tattgcccca agtgattctg ccacagagcg tagcggcaca agcgttctcc cgtttcttat
     6661 caatggacca acgtctattt tgactacctt gttgtcaaat tccgccctat cttctccaat
     6721 ccagtatttc aggactttcc ccttgcggct tatctcaacc tttttttctg tcgcattcca
     6781 gtcgactttg cacccgaaag tctctgcaat aaatcttatt ggaacaagag ttctcccttt
     6841 ataaacctcc gcaggtacat caagcaaaac tttgccacta tttatcaatg cctcacttga
     6901 accaggttcc atctgtatcc ggagccttgg atcggtgaca aaatcaagtg aaagttcgtc
     6961 ttcccatgac gacatgcccc cgtttctttc cgggcatgtg ttgcacgtta tttttattgg
     7021 tacagaaaaa tcggaacctc caggaaggtc catgcagtta aagctgaaag aaaaggaggt
     7081 tgttccattc tcttgatact ttccaccctg gccatcaacc ttcaaccagt caggtttatc
     7141 aatatccagc ctgatatatc tgtcttcacc gtcagttgaa ttgatcctca cctcaaatcg
     7201 tccctgggat gagcggggaa aatatggaac atcagatgga ctaatcgtaa cgtcaaagca
     7261 tggttcacag gtttgacatt tcttttggga gctcacaata tccaccgtga aaaaaaccag
     7321 gacccatcca gatattcctg gttcactgta ctgtccattg ccctccatga cagggcaacc
     7381 aggctcaatt gcaacaccaa acttctgttt gccatcgcaa aaagaaggat cagaggggtc
     7441 aatgatcatt gtgtatttat aaccccttct catgccttca gaaaatgtct tgccaccaat
     7501 aaaactgacc gacttgcacc tgcttccggc ctcaaagggc atcccctcaa acttgggtgg
     7561 tggcagtctg ccatcctctc ttccaaaaag aagcgtaaaa gggaaccttt ttgtgccatc
     7621 tgaagctacg attgtcccga ggtccctgtt ttccaggacc tccgcatacg ggcactcttc
     7681 ccagcaaaac aactggccgt cagaggtcac ataagagatc ctgccacctt ctatcaaaac
     7741 agacgatctt gttgcatgga ccagcttcct tttccacacg acagagcctt cgggatcaat
     7801 cccccaaaag gttttgtttc catcaataaa gtaaatgctt tggccatcag ttgccaacgg
     7861 ccaggagact gggccaaagc cttccttctt ccattcctgc ctgccagttt cggcatcaac
     7921 tgcatataaa atgccggatt catctgcaaa gatgcatttg cttccaacca gcactggatt
     7981 tgaccggatc ggcgcctccg caatgtagcg ccagagtaat gatccatcat cagcattgag
     8041 acaatacagg acatggtcca gagaactggc aataaccttt tgcccaaaaa tcaaaggttg
     8101 tgtctcgatc cagccaccag tttcaaagga ccagatctct tttccttccg gaacagaaac
     8161 acgtctcaat ctgttgtcat cgccaccaaa aataactgat ccagagtaaa tcgcaggtgt
     8221 tccccatatc ggagattttg aatcaaacct aaaaacaaga gaaccatctg tggcgttaag
     8281 gcagtagaga ttgccatcgg aacacccaaa aatgatcttt ccatcaaaaa gcactggcat
     8341 tccgtatatt gcattgccgg ccctgaaact ccagactatt tttttgccaa gcaaatcaac
     8401 aagcgcgagt gttccagctt tggtcccgac aaaacagctg gcgttataaa cgagtggcga
     8461 gcttgcaata tcggcaccgt caatttcaag ctcgaatttt ttcatgccag atttaatata
     8521 gacaagcttt ccactggcat ttccaataag gtagccagaa tcgaaaaaag tgggtgaata
     8581 ggctgaaaga tagcttccac caacagaaca ttttaaggca acctgaaaag gcgcggtggc
     8641 aattggtttt gcatcgccag cagcaggcaa aattgcctga gaaggaacaa taaacatcag
     8701 agcaaccaaa aatgaaattt tcttcacatc tcaaatacgt caaaaaaccg aacatagttt
     8761 caagtcaatt ttcttgccgg cttaacaaca gattgacaag agtgtcccaa aggttattgt
     8821 tgagctattg aggaaaaaac ttctatcttt tgttgtcctg ggagtgggag caatcatcgc
     8881 acaaatagtt gttgccctgt tttattcggg aaacagcgag aacctctggc aaattctggg
     8941 atttggaaat ttatttgtgc taattgcctg ctttatggca tccttcaaga ataaactgca
     9001 aatactacca ctacttgggt tgataattgg aatatcaagt cctgttctta gtttcatctg
     9061 gatgggaaga ggttttgctg gacaggcaat ttcatactcg ctctcatatt tttgtttttc
     9121 catgcactat attctccaga gagaaaaacc cctaaggtat ttcggatggc cccttctgct
     9181 atgctctttc ttgctttggt ctttccatat ttttctggca aatgcaggat ctggccttcc
     9241 accgtttgac ataattgcat tcttcttcag cctgctgatg cttttggcgg catcaagcat
     9301 tctgctgatc atcatttttg aaaaaaatac aatacaggag gacatttcat gaaccagatt
     9361 ctgaagagca agtttaatgc gtggggattt tttattgtgg cgtgtgtgtg ggtattgatg
     9421 atgatttgta gtctgatgtt ttctattcta gacatcaacg ttgatgtact gtccccaata
     9481 ataaggatgc taatggttgg aacaggaatg gtaatgactt tcttcttctt ttccatctca
     9541 aggtcagagc cagggcttgc ggcagttggt ttggtgctag gaatagttgg aacaatagcc
     9601 caaatatctc caacagagag tgggttttct gaaatcgtag gatttattat cggaatcagc
     9661 tacatctttt tcgccttcgc catcctgaaa caggaagcaa aggcagcagg ttggttaggt
     9721 tgggcaatga ttctttcggg aatcataaaa ctgtcttcaa ttggaatgat tactggaatg
     9781 atgagaggcg aactgggcgg cataattatc ttctctgcaa taacaatcct ctttgagatt
     9841 ttcgtcgcac tcgcagcaat ttactacatt atagttgggg gcaatctatt cgaaggtact
     9901 ggcgcaccaa aaacaatcaa cgctccagag aatttgaact gaaactaact tatttatacc
     9961 aataagggag gttgcatttt ttgtgtaaaa tagatgtaac ctccccctca aaaaaca
//
LOCUS       DAOSRI010000026         9897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6612_length_9897_cov_3.434058, whole genome shotgun sequence.
ACCESSION   DAOSRI010000026 DAOSRI010000000
VERSION     DAOSRI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6612_length_9897_cov_3.434058"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PK717_01410"
     CDS             <1..1675
                     /locus_tag="PK717_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOR46359.1"
                     /translation="TTEQAFGEKLHTYTIVDAINDRNVLPFRIDFINTIKKPDHIDDK
                     KVHSIDTEKALADPQRISEIVAYVLDHFDQKTKRSSFYTLSAKWEEAGKHDPKTTIEK
                     CETRRVAGFNSIFTAASIPIAIKYYTEFKKQIAEKHRNLTIATIFSFSANEDEPGGML
                     PDEGFNMDNLDQGSRNFLETAIKDYNAIFSTNFDTSSDKFQNYYKDLSLRVKNREVDL
                     LIVVNMFLTGFDATTLNTLWVDKNLKQHGLIQAFSRTNRILNSVKTFGNIICFRDLKE
                     ETDKAIALFGNKDAGGIVLLKTYDEYYNGYDENGEHRPGYKELVADLKTQYPLGQPIL
                     GEEAEKDFIQLYGAILRLRNILSSFDNFAGNEVLHEKNFQDYQSIYIDLYQKIKKGAD
                     GDRETINDDVVFEIELIRQIEVNIDYILMLVEEYHESNCKNKNILTTIDKAINSSIGL
                     RSKKQLIEQFIEQINASTKVDEDWRKFLCERKEADITTIIEEERLKPDETRHFVDNAF
                     RDGSLKTTGTAIDKIMPPVSRFGGGNRATRKQSIIERLLKFFEKYSGLL"
     gene            complement(1732..2835)
                     /locus_tag="PK717_01415"
     CDS             complement(1732..2835)
                     /locus_tag="PK717_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR46360.1"
                     /translation="MEHPANMQGHGHPGGMPQGNGIMPPFLVSYSVTRECNLRCKHCY
                     SESSDNVPKDELTTEQAFRLLDEVVSWGPKLLIFDGGEPLKRADFFDLAKHASSKGIR
                     TVIGTNATLIDRGVAQKLKDCGVMAAQISVDGANAKTHDWFRGVEGSFEKAMQGAKAC
                     REVGLPFQFGATIRKSTIKELPRLLDMAVETGAIAAELFDLVQVQRVKEEIPDEILSV
                     EERKETMGWLAKKQEDYPIVIRTPGCTMYPITLQSAGIVSKHFPKENLMRIPYYNRGC
                     AAGMPNGYVTILPNGDVIPCMLLQTVVGNVKNDSLKKIWETSPVLLELRDRSKLKGKC
                     RGCEHDKTCSGCRGRAYEHTGDYLAEDPGCYLK"
     gene            complement(2913..3602)
                     /locus_tag="PK717_01420"
     CDS             complement(2913..3602)
                     /locus_tag="PK717_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOR46361.1"
                     /translation="MARCVNLIDGGYFERVQKALGRGKIDFLKLQEKMTGNFDMLRTY
                     YYHCLPYQSANPTEEEKLEFARKQSFFKYLEYQPNFEVRLGRLEFRGYTQTGERILEQ
                     KRVDLLIGVDLVMLSMKKLITHACIIAGDSDFIPAIEVAKYEGVITYLFHGPNCHEDL
                     KQIADVRIEINDEFMDAVMAQDVAKPQRKVFETVEEYEKKAKKPHSIWKKEAEVVQEV
                     KKTRKGKTAKP"
     gene            3757..4317
                     /locus_tag="PK717_01425"
     CDS             3757..4317
                     /locus_tag="PK717_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46362.1"
                     /translation="MKKIALLVAVCITVCFFASCGGSATSNDVDIYVRLDMAKRLLEL
                     VDFKFGKMAPDQACIGVLSILKADYSKVPSSLYEDLQKTLKDKGGQDFRRELAKAFGE
                     YEGKIGKVQDAIEKETTIATLSGAKSFLVANETLGMVKGTGIEEVNWIIQYIKHLPEE
                     ITTKLAGIKNLAGYYKAIDEFIEKYR"
     gene            complement(4387..7464)
                     /locus_tag="PK717_01430"
     CDS             complement(4387..7464)
                     /locus_tag="PK717_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46363.1"
                     /translation="MRKYGRSFAVVMLVAFLMTPLFSISEIRCPDEISAAPASFWAID
                     GGDPGRTGSANASCGPSTTSLGQLWQGVNDKAFFSIISDSKTLYTVSRDPDIGGYYEL
                     RCYDYATGGYLWGQGPKAYISRPCLSGRMLYFISYDISYTSSLTCFDTVKRKPVWEFT
                     IEDQVESDPLCVGGKVIFVYGQTVLALDAATGKKKFSCDLDYSPLPRLASNGNAVFAA
                     MPDGSVNSIDLSTGEILWTANGALGEISEEDKMAISALQDTVFVSRSGDDSLIVALDS
                     KNGDKKWEKKFEKTFLDSFVLWQGKVIANLFDEKGQVLYALSQTDGSRFYFTNIRNTA
                     PKGDTQISIGTMSVFGDYLIMPTRSNVVCVMGALSGMERYKIKTQELPSVVIPTLGRI
                     YAGSGNNIECFGQMPEGGLYISPTKLDFGEVPKYQAAQASFTIKNCLPEKVSVTLSQG
                     FDWATLSVKSVVIESQKRFVVDIVTVPIKLASAGQKNGTVKVSWNSRSVVVSVAAKVV
                     DKPIDNNWLMPRYNEKKVGIPPKGTSPNTPHISVIWSAQLPKASGLSPFAPVVYKGKA
                     YILYGKTLAGFDMVSGKKALSVSLPFTAISSPVVSDSKLYVSGKSSSTDLYCLNCANG
                     KVIWTAKGKGSQTSHVVCEGKLYGTFQGTIYCLNAYDGKQVWSKSFAGASFFSPSIGQ
                     GLVYCWSVDPNGKWSLSCFSAKNGNLVWSYDYVQKLSLSRPQNGEYFAPVIFKDMLYT
                     VMPEREGSSVAIFDAKTGSLLRNFKFTGSSEADTKFPTFGSMPFTGSGKIIALSGERP
                     TVFGFDEETGAQVWRKNIAQKSDLLTPINPALTGSDFSQGYLVIGYARSLQCINPANG
                     KTIWTLPVGLDYCSSPAISSGKIIVFGNDGKLICFGESPFAKKKLVFVLGQKSFKRDS
                     FTYPTELGVVKLDGVLYAYAANIVEPLLGTVSWVPKESKLTCKLGKNTVEFWLGNTKA
                     KVNGKLVPIDADPKIVPKASQGIMHVPLAFLCKSLKAAMSFDSKTGTATISYP"
     gene            complement(7560..7925)
                     /locus_tag="PK717_01435"
     CDS             complement(7560..7925)
                     /locus_tag="PK717_01435"
                     /inference="COORDINATES: protein motif:HMM:NF021218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, LLH family"
                     /protein_id="HOR46364.1"
                     /translation="METVWEILGNEAVVTTLVAILCTVLGWVAVKLRLYLMSKLGKEK
                     LDRALEMAEIVATGVEQMAERLGWSSKEKFDEAQQKLKGWAKSCGISFTDAQWESIIE
                     KTVLELSSVWRQLKGENAP"
     gene            complement(7935..8237)
                     /locus_tag="PK717_01440"
     CDS             complement(7935..8237)
                     /locus_tag="PK717_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46365.1"
                     /translation="MEENGNLEERIEGKLDAIWDKLSGLSVLCERALVSLEWLDKRTS
                     KLEVDTHDPSKCPVQTGIEGVKRDQASLSRTVGWIMGLIATVITGTAVYFVTQLVR"
     gene            8506..9393
                     /locus_tag="PK717_01445"
     CDS             8506..9393
                     /locus_tag="PK717_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR46366.1"
                     /translation="MKKKSTKIDPKRAKAISTIKALLFSASVISSLLAGAIAYRLGSF
                     HPTNYAIMFFGVVLGQLSGDYLYYYFTNYHTSTKDTHTKLFAGWKPYFAETLIKGKQN
                     LYAGLGILFVDLLIGLYFIFKAGLALAIFVASGGLIVLLFTPLMFYGLKEVVVFLAFG
                     PLAMTGMIFVLTGKFSWETLIASIPVALWVTAVAHLKSGKIKKIDERAGTVSLTVSRN
                     IITALLLLSYVSLGLGVAFAYIPVWSLLALLTLPLSINVVMDLGKPSNTIVSYLNSTI
                     KTIFVLVCGGLLMSAGYLI"
     gene            9406..>9897
                     /locus_tag="PK717_01450"
     CDS             9406..>9897
                     /locus_tag="PK717_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR46367.1"
                     /translation="MKKFANWFVLWFKAARAPFLITSLIPSILAGMIARSHGLFSWLP
                     FVLATVGVVMAHSAGDFFDDYYDFKKGVLGHKDEQFHDSPLIDGKVTPGQVLAAGIGC
                     AIVALACGAYFLFTIGLPVVYMAIAGGIIAVFYTAPPISLNFRGFGETALFFAFGPLV
                     TLGV"
BASE COUNT         2736 a         2342 c         2086 g         2733 t
ORIGIN      
        1 aacaaccgaa caggcctttg gagaaaaact gcacacctat accattgtgg acgccattaa
       61 tgacaggaac gtactgcctt tcaggataga cttcatcaac accatcaaaa aacccgacca
      121 tatagatgac aaaaaagtcc attccataga cactgaaaaa gccctggctg atccacagcg
      181 aatcagtgaa attgttgcct atgttcttga ccatttcgac cagaagacaa agcgcagcag
      241 tttttataca ctttctgcca aatgggaaga ggctggaaaa catgatccca agacaacaat
      301 tgaaaaatgc gaaacaagac gcgtggccgg tttcaactcc atatttactg cagcgtcaat
      361 tccaatagcc atcaaatatt acaccgaatt taaaaagcag attgcagaaa agcaccgcaa
      421 tctcaccata gcaaccattt ttagtttcag cgcaaacgag gatgagccag gcggcatgtt
      481 gccggacgag ggtttcaaca tggacaatct cgaccagggc tcgcggaact ttcttgagac
      541 agcaatcaag gattataatg caatattcag cacaaatttt gatacatctt cggacaagtt
      601 ccagaattac tacaaagacc tttcccttcg cgtgaagaac cgcgaggttg acctgctgat
      661 tgtggtcaat atgttcctga caggttttga cgcgactacc ctcaacacgc tctgggtaga
      721 caaaaacctt aagcaacatg ggctgattca ggcattctcg cgaacaaacc gtattttaaa
      781 cagcgtcaag actttcggca acatcatttg tttccgcgac ttgaaggagg aaacagacaa
      841 agcaattgct cttttcggca acaaggatgc aggcggtatt gtgctgctca aaacatacga
      901 cgagtattat aacggctacg atgaaaacgg cgagcacaga ccaggctaca aagaactggt
      961 tgccgatctt aaaacacaat atcctcttgg acagcctatt cttggtgaag aggcggaaaa
     1021 agattttatc cagctgtatg gcgcgatttt aaggctgagg aatattttgt cttcctttga
     1081 taatttcgca ggcaatgaag ttttacatga aaagaatttt caagattacc agagcatcta
     1141 tatcgacctt tatcagaaaa tcaaaaaagg cgctgacggt gatagagaga ctatcaatga
     1201 tgacgttgtg ttcgagattg aacttattcg gcagattgag gtcaacattg actacattct
     1261 catgcttgta gaagaatacc atgaatccaa ctgcaagaat aagaatatcc tcacaactat
     1321 cgacaaagca atcaattcca gtatcggact tcgcagcaag aagcagctta ttgaacaatt
     1381 catagaacag atcaatgcat caacaaaggt cgatgaggat tggcgcaaat tcctgtgtga
     1441 acgcaaagaa gcagacatta caacgatcat agaagaagaa agattaaaac ctgacgagac
     1501 tcgacatttc gtggacaatg ctttccgcga tggttcactg aaaacgacag gcacggccat
     1561 tgataaaatc atgccgcctg tatcgcgttt cggcggtggg aatagagcca caaggaagca
     1621 gagtataatc gaaaggcttc tcaaattctt tgagaagtat tctggattat tatgaacaca
     1681 gatgtttcgc cttagtaact tttaggtttt tatgatttgg cactgattga attatttgag
     1741 gtagcaacct gggtcttccg caagataatc gccggtgtgc tcgtatgccc ttccacggca
     1801 accggaacat gttttgtcat gctcacaacc tctgcattta cccttcagct tcgatctgtc
     1861 gcgaagctca agcaggacgg gcgatgtttc ccaaatcttc ttcaggctgt cgttttttac
     1921 atttccaacc acagtctgga ggagcatgca cgggatcaca tctccgtttg gcagaatggt
     1981 gacataaccg tttggcatgc ccgcggcgca gcccctgtta taatatggga ttctcattag
     2041 gttttctttt gggaaatgct ttgacacaat gccagctgac tggagcgtaa ttggatacat
     2101 tgtacatcca ggtgtcctta tcacaatcgg gtagtcttcc tgctttttgg caagccatcc
     2161 cattgtctct tttctttctt ctacagaaag aatttcatcc gggatttctt ctttgaccct
     2221 ctggacttgg acaagatcaa aaagttcggc ggcaattgcg cctgtctcga ctgccatgtc
     2281 caacaacctt ggcaattctt ttatcgtgct cttgcggatt gttgcaccaa actggaaagg
     2341 aagtccaact tcacggcagg cctttgcacc ctgcatggct ttttcaaacg aaccctccac
     2401 gcccctgaac cagtcatggg tttttgcgtt tgcgccatca accgatatct gcgcagccat
     2461 cactccacaa tctttgagtt tttgtgcgac acccctgtcg atgagggtgg catttgttcc
     2521 gatgaccgtt ctgattcctt ttgaactagc atgcttggcc agatcaaaaa aatctgccct
     2581 cttgagaggt tctccaccat caaaaataag gagtttcggt ccccatgaaa cgacttcatc
     2641 aaggagcctg aaagcctgtt ctgttgtaag ctcatccttt ggcacattgt cggatgactc
     2701 actgtaacag tgcttgcacc tgagattgca ttcccttgtt accgaatatg agacaagaaa
     2761 gggcggcatg atgccattgc cttgtggcat tccacctgga tgcccatgtc cttgcatgtt
     2821 tgctggatgt tccatataac ttccccctgt tggcaggata tgggacaagc ataggccaga
     2881 aaaattctca gattaagccg gtcaacctta aattaaggtt ttgcagtttt accctttctg
     2941 gtttttttca cttcctggac tacctcggct tcttttttcc agatactatg gggttttttt
     3001 gccttttttt cgtattcctc taccgtctcg aagacttttc tttggggctt agcgacgtcc
     3061 tgagccatga ctgcatccat gaattcatca ttgatttcaa tcctcacgtc tgcaatctgc
     3121 ttcagatcct cgtgacaatt tgggccgtgg aaaagatacg ttattacgcc ctcatacttg
     3181 gccacctcaa tggcaggaat aaaatcggaa tctccagcga ttatgcaagc atgggtaatc
     3241 aacttcttca tggacagcat tacaaggtca acaccaatca aaagatcgac cctcttttgc
     3301 tccaaaattc tctcaccggt ttgagtgtag cccctaaatt caagccttcc gagtctcact
     3361 tcaaaattgg gctgatattc aaggtattta aaaaaagact gctttcttgc gaattcaagc
     3421 ttttcctctt cagttggatt tgcgctctgg tatggaagac agtgatagta atacgtcctg
     3481 agcatgtcga aattgccagt cattttttcc tggagcttca gaaaatctat ctttcccctt
     3541 ccaagcgctt tctgcaccct ctcaaagtat cctccatcaa tcaaattgac acatctagcc
     3601 aaaagaacct ccttcatata aaaccccgac ctcgtttgtt cccaacgccg ggggccgtat
     3661 aatatagcac accttaataa acccaaaaaa tcttttagtc aagcgtcaaa gttctgcttt
     3721 gtttgaactt gggcattttt tgtatataaa atcatcatga aaaaaatagc tttgctggtt
     3781 gcagtatgta ttactgtttg ttttttcgct tcctgtggag gcagcgccac atcaaacgat
     3841 gttgatatct atgtaagact ggacatggca aaaaggttgc ttgaacttgt tgatttcaaa
     3901 tttggcaaaa tggcgccaga tcaggcttgt attggtgtac tctcaatcct caaggctgat
     3961 tactcaaaag taccatcatc actctacgag gacctgcaaa aaacgctaaa agacaaaggt
     4021 ggtcaggatt tcaggagaga gcttgccaaa gcttttggcg aatatgaggg taaaatcgga
     4081 aaagtgcagg atgccataga aaaagaaaca acaattgcaa cgctttcagg tgcaaagagt
     4141 tttcttgttg caaacgaaac gctcggcatg gtcaaaggca ccggtataga agaggtcaac
     4201 tggataatcc agtatataaa acatctcccc gaagagataa cgacaaaatt ggccggcata
     4261 aaaaacctgg ctggatacta caaggcaata gacgaattta ttgaaaaata tagataaatt
     4321 cagcgatccc ctaataaaca aaaaaccctc cttttagggg agggtttttt attgcgacag
     4381 aactttttat gggtaagaga ttgttgcagt gccagttttt gaatcaaacg acatggccgc
     4441 cttgagcgat ttgcacaaga aggcaagcgg aacgtgcatg attccctgcg atgcttttgg
     4501 cacaattttg gggtctgcgt caattgggac aagttttcca ttgactttcg cctttgtatt
     4561 gccaagccag aattcgacag tatttttgcc aagcttgcat gtcagtttgc tttcctttgg
     4621 cacccacgag actgtcccaa gcaggggttc cacaatattt gcggcatatg catacaggac
     4681 accatcgagt ttcacaacac caagctcggt tgggtatgtg aaagaatctc ttttaaacga
     4741 tttttgcccc aggacaaaca caagcttctt ttttgcaaaa ggcgattccc cgaaacaaat
     4801 taactttcca tcattcccaa aaacaatgat cttgccggag gaaatcgcag gactggagca
     4861 atagtcaaga ccaacgggca atgtccatat cgtttttcca ttggccggat tgatgcactg
     4921 aagagacctt gcatacccta tgacaagata accttgggaa aaatcactgc cagtaagagc
     4981 aggatttatg ggggtcaaca ggtcgctttt ttgggcaata ttttttctcc acacctgggc
     5041 gccagtttct tcatcaaacc cgaaaacagt aggtctctcg ccagacaggg caatgatttt
     5101 cccagagcct gtaaaaggca tcgagccaaa ggtggggaac tttgtgtcgg cctctgatga
     5161 accagtaaat ttgaagtttc tgaggagaga gccagtcttg gcgtcaaaaa tggccacaga
     5221 tgaaccctcg cgctctggca ttacagtgta aagcatgtct ttaaatatga cgggcgcaaa
     5281 gtattcgcca ttttgcggtc tggaaagtga tagtttctga acatagtcat aggaccaaac
     5341 caggttgccg ttttttgccg aaaagcaaga cagggaccat ttcccattag gatcaacaga
     5401 ccagcagtag acgagcccct gtccaattga tggagaaaag aaagaagcac ctgcaaaaga
     5461 ttttgaccag acctgcttcc catcgtaggc attaagacaa tagatcgttc cctggaaagt
     5521 tccatacaat ttaccttcac agacaacgtg cgaagtttgg gagccttttc cttttgctgt
     5581 ccagattacc ttgccatttg cgcagttgag acagtagagg tctgttgatg aagatttacc
     5641 agatacatac agcttgctgt cggagacaac cggggaactt attgcagtaa acgggagtga
     5701 aaccgagagc gctttcttgc cactgaccat gtcaaaaccg gcaagtgtct tgccatacaa
     5761 aatataggcc tttcccttgt agacaaccgg tgcaaacgga gacaagccag atgccttcgg
     5821 caattgtgca ctccagatga cagaaatatg cggcgtgttg ggcgatgtgc cttttggtgg
     5881 gataccaacc tttttttcgt tgtatcttgg cataagccag ttgttgtcaa taggcttgtc
     5941 aacaaccttt gctgcaacac tgacgacaac cgatcgtgaa ttccatgaga ctttcacagt
     6001 accatttttt tggccggcag atgccagctt gatggggaca gtcactatat caacaacaaa
     6061 ccttttttgt gattcgataa ctacggattt gacagaaagc gttgcccagt caaacccctg
     6121 tgaaagcgta acagacacct tttctggcag gcaatttttt atggtgaaag atgcctgcgc
     6181 agcctggtat ttcggtactt ccccaaaatc cagctttgtt ggagatatgt agagcccgcc
     6241 ttccggcatc tgtccaaaac attctatgtt gtttccagag ccagcgtaaa tccttcccag
     6301 agttggaatt acaacagacg gtaattcctg ggttttgatc ttgtagcgct ccatgccaga
     6361 aagtgctccc atcacacaaa ctacgttcga tcttgtgggc attatgagat aatcaccaaa
     6421 aacactcatt gtaccaatgc ttatttgtgt atctcctttt ggagcagtat ttcttatgtt
     6481 tgtaaaatag aatcttgaac catcagtttg cgacagggcg taaagcacct ggcccttttc
     6541 atcaaagagg tttgcaatca cctttccctg ccagagaaca aacgaatcaa ggaaggtttt
     6601 ttcaaatttt ttctcccatt tcttgtcacc attcttggag tcaagcgcaa cgatcaggga
     6661 atcatctccg gagcgggaaa caaaaactgt gtcctgcaaa gcagatatgg ccattttgtc
     6721 ttcttctgaa atctcgccaa gtgccccatt tgcagtccag agtatttctc ctgtcgaaag
     6781 gtcaatcgaa tttacggaac catcaggcat tgcagcaaaa acagcgtttc catttgaagc
     6841 aagccttggc aatggcgaat aatcaagatc acatgagaat tttttcttac cagttgcagc
     6901 atctagggcc agcacggttt gcccataaac gaaaataact tttccaccaa cacacagtgg
     6961 atctgattct acctggtctt caatggtaaa ttcccagact ggtttccttt tgacagtgtc
     7021 gaagcaggtg aggctgcttg tataggaaat atcataggaa atgaaataga gcatccttcc
     7081 agacaggcat ggtcttgata tgtatgcttt tggaccctgc ccccaaagat aacctcctgt
     7141 tgcataatca tagcatctca gctcatagta tccgccaata tcgggatctc ttgataccgt
     7201 ataaagcgtc tttgaatctg atataataga aaagaaggct ttatcattga ctccctgcca
     7261 gagttgacca agtgaagttg tagatggacc acaagaagca tttgctgatc cggtccttcc
     7321 gggatctcct ccatcgattg cccaaaaact ggcaggtgca gcagaaattt catcaggaca
     7381 acgaatttct gatatactaa acaaaggtgt catcaaaaat gcaaccaaca ttacaacggc
     7441 aaaacttctc ccatattttc tcatcaatac caaggataac cgtttgtggg gcattgtcca
     7501 gaaatcatca aaaaggcggc atgcatgccg cctttttttg ttattgagct tttattaatt
     7561 tacggagcat tttcgccttt gagctgtctc cagacacttg aaagctccag gacagtcttc
     7621 tctatgatcg attcccactg ggcatctgta aagctgatcc cgcaagattt agcccagccc
     7681 ttcaattttt gctgcgcctc gtcaaacttt tcctttgagc tccatccaag tctttcagcc
     7741 atctgctcaa cacctgttgc aactatctcg gccatttcga gtgccctgtc gagtttctct
     7801 tttccgagct ttgacatgag atagagcctc agcttgactg caacccagcc aaggacggtg
     7861 cacaaaatag ccaccagcgt tgttaccact gcctcattcc ccaaaatctc ccagactgtc
     7921 tccatgttcc cctcctatcg aaccagttgc gttacaaaat aaactgctgt ccctgtaatc
     7981 accgtggcta tgagccccat gatccagcca actgttctgg aaagtgatgc ctggtccctt
     8041 ttgactccct cgattccagt ctgcaccggg cattttgacg gatcatgagt gtccacctcc
     8101 aattttgaag tcctcttgtc gagccattca agactcacaa gcgccctctc acacaaaact
     8161 gacagcccag acaatttgtc ccagattgca tccagcttac cctctatcct ctcctcaagg
     8221 ttcccgtttt cttccaatat caaccccctt cttcattggg gtgcaggcaa atatcatatt
     8281 gacaaagttt gagccaaaaa gaatgatcgg gttttcaaaa tctgtgtcgt tgccctagga
     8341 tggcgagtca gcccccaatg gaactacaaa ccaaatcatt agattggttc aaagacacag
     8401 tcttcggcaa aaaatccttg cgatcaacta taaaatcaaa tgttcaacct taatggtctt
     8461 tccagtacaa tgataaaaaa tcactcacca ggacaagaca caataatgaa gaaaaaaagc
     8521 acaaagattg accccaaaag ggccaaggca atttcaacaa tcaaagcatt gcttttctcg
     8581 gcatcagtca tctcgtcact gcttgcaggc gcaatagcat acaggcttgg ttcattccat
     8641 cctaccaatt acgccatcat gttttttgga gttgtcctcg ggcagctttc aggagactat
     8701 ctttactact atttcacaaa ctaccacacc agcacgaaag acacccacac aaaactcttt
     8761 gccgggtgga agccatattt tgccgaaaca ctgatcaagg gcaagcaaaa tctttatgcc
     8821 ggcttaggga tattgttcgt tgatctcctg ataggcctgt atttcatatt caaggcagga
     8881 ttggcacttg caatctttgt tgcatctgga ggactgattg tcctgctttt cacgcctctt
     8941 atgttttacg gactaaagga agttgttgtt ttcctggctt ttggcccact ggcaatgact
     9001 ggtatgattt ttgtgcttac gggcaaattt tcctgggaaa ctctcattgc atcaatacca
     9061 gtagccttat gggtaacagc agtcgcacac ctgaaatcgg ggaaaatcaa gaagatcgac
     9121 gagagggctg gaacggtgtc gctgacagtc agcagaaata taattactgc attacttctg
     9181 ctgtcgtatg tgtcattggg gcttggtgtc gcatttgcgt atatcccagt atggtccctg
     9241 cttgcccttc tgacattgcc actctcaatc aacgttgtca tggacctggg caaaccatca
     9301 aacacaatcg tttcatacct caactccacg atcaaaacaa tatttgtgct tgtttgtgga
     9361 ggattgctaa tgtcagcagg atacttgatc taggagaggg aacgcatgaa aaaatttgcc
     9421 aactggtttg tattgtggtt taaagccgca agagcaccgt tccttattac cagccttata
     9481 ccatccatcc tggcagggat gatagcaagg tcgcatggcc tgttttcatg gctcccattc
     9541 gtgctggcca ccgttggtgt cgtcatggcc cactccgcag gtgatttttt tgatgactac
     9601 tatgatttca agaaaggtgt tctgggccac aaggatgaac aattccatga cagcccgctg
     9661 attgatggaa aagtgacccc tggccaggtg cttgcggccg gcattggctg tgcaattgta
     9721 gcgctggctt gtggtgctta ctttctcttt accatcggtc tgccagttgt atatatggca
     9781 attgcaggtg gcataatagc tgttttctat actgccccgc caatatctct gaattttagg
     9841 ggattcggtg agaccgccct cttctttgca tttggtccgc ttgtgacact gggtgtt
//
LOCUS       DAOSRI010000027         9642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6836_length_9642_cov_4.492855, whole genome shotgun sequence.
ACCESSION   DAOSRI010000027 DAOSRI010000000
VERSION     DAOSRI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_9642_cov_4.492855"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..628
                     /locus_tag="PK717_01455"
     CDS             101..628
                     /locus_tag="PK717_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46368.1"
                     /translation="MNIGIIRISTKADRYRLKQTIESIGVQVVQTIDSASMPPTIVFV
                     GTEDQMLMAFKQAENLGAEYAKVLTHIREVSPKLVKTLKEGPKFKPGQPFGILETAGP
                     EAILACANLAAMFDGIELAEIQLASMPNEKSLMVITGDKKLIENVLESAEMICEKAHF
                     TVPGSKPWQNDGRQN"
     gene            652..1113
                     /locus_tag="PK717_01460"
     CDS             652..1113
                     /locus_tag="PK717_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOR46369.1"
                     /translation="MDFIKSKKPEHCVFCKAGTNIGGEDKDFIVYRGEKCFVILNRYP
                     YATGHIMIIPYRHCQKFEDLGNEELSEMMILAKKFVSVLKRAFTPDGFNMGMNLGAAA
                     GAGIADHIHLHIVPRWIGDTNFMPIFAEVKIHPTDLEGVYKAILQALEATA"
     gene            complement(1103..1378)
                     /locus_tag="PK717_01465"
     CDS             complement(1103..1378)
                     /locus_tag="PK717_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46370.1"
                     /translation="MPQWIESLVNLIFSLTIVSLGIVAYKKHGARAFLIISIAFLCFA
                     LSHAVRFGENIFGYPVVENSILSVALVLVRITGYALVVFGLAISFRR"
     gene            complement(1385..2425)
                     /locus_tag="PK717_01470"
     CDS             complement(1385..2425)
                     /locus_tag="PK717_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR46371.1"
                     /translation="MLSGEINGTGLLDLFKFLLSQNQTGTITVSNASGSGSVQVRSGE
                     ITFAKWQEKIAEQAFYEIIKLDLLKFTFEQGYAIETNIKTLTESLLKAASVYINLQNR
                     ICQSTVLVRNPLKEGGQIAINPDKWLITGLIGSGITYSNLVSSLSFSEEETKKLVNEM
                     LDEGMIAIKKAEIKKDIFLVPRVTKTKLGINESEILSKITIGTRLVDLFESMSLSIDD
                     FADMMCILCLKNAISVIDEYSKVIDMSRFYDTLGIQKIAHQKELVISVGNTRHGQVFV
                     DEYLYDMWNARLRGQKIDSVEFKNIYYKPVFSVIKMRNLDEKVVIAQIDANKLDLKDG
                     DTISCVPCRKGD"
     gene            complement(2483..3514)
                     /locus_tag="PK717_01475"
     CDS             complement(2483..3514)
                     /locus_tag="PK717_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46372.1"
                     /translation="MHSGEIVGGELYDLLAFFLNQGHSGTIIFETDSCSGDIVVDKGN
                     IAMAKFGDLTGKDAIMALSKIGSLRFNFTRLNKQHCEKPIPFKYLSKINKLSPDERFL
                     PRTIVQKTERVLPNVSLSIQKENILNSLLTPLSVAQLAISTKLSLPCLQETLSSMVEE
                     GLVFIKPKFMVDALFLFPKESPIDLSEREKQVFNSIAHGMTLGEFLAQYENQTVSIAD
                     ILVSLVLKKMVVMSDDNGKIMPPWYIYKSLGFEKVARPILLKLRIDNSLCVRPNTLAI
                     DEFVIGMWESQLFGKKIDSVEIVCPNGKVSFLVEKGKMPRDSFFIRPNDQKLLDVLEG
                     SFVNIYPKY"
     gene            3707..4810
                     /locus_tag="PK717_01480"
     CDS             3707..4810
                     /locus_tag="PK717_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOR46373.1"
                     /translation="MKAVQKTEPKIGAKLVEVPTPTNLKPGWTLVKVRATSICGTDIH
                     IWNWDQWSQGRIGEKALPQILGHEFCGEVVELGPNCNRIKVGDYVSAETHIYDPGDLT
                     SMLGIFHVGNNMKILGVDTDGCFADYVAIPEIVLWKNDKTMPPEYASIQEPLGNACYA
                     VMGEDNDVYGKTMLITGDGPISLFAIGVAKAAGASKIIQFGMAQYSMEIGRKMGVDIQ
                     IDVTKTTPDERYKIVMDNTGGWGCDIVCEMIGAQSSLDDAFRCIRKGGRFSAFGIPSK
                     SPSQIDYTDQIVFKGIQIHGISGRKIFDTWYRNGNMLANRKLDPTPVVTHLMALEDYQ
                     KAFEMLTAPTRQCAKIVMFPDKQELNAALARRK"
     gene            4822..6072
                     /gene="kbl"
                     /locus_tag="PK717_01485"
     CDS             4822..6072
                     /gene="kbl"
                     /locus_tag="PK717_01485"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOR46374.1"
                     /translation="MAYGKIKDFLTNELKGIRDAGLYKEERIIVTPQAADIKVQFPEN
                     APVKEALNFCANNYLGLSSHPDVLKAAKEGLEMRGYGLSSVRFICGTQDIHKKLERKL
                     SEFLGVEDSILYSSCFDANGGLFEAIMKEDDAIITEELNHASIIDGVRLSKAKRYVFK
                     HSDMNDLEAKCKEARGAGARHMIIATDGVFSMDGDIANLKGICDVADKYDALVMVDDS
                     HATGFIGKTGRGTHEHNGVMGRIDVITTTLGKALGGASGGATCGRKEIIEILRQRSRP
                     YLFSNTIPPVVASAALRVMDILSSTTERRDKLERNTMYFREKMTSAGFDIKPGVHPIV
                     PIMLYNAKLSQDMAKDLYEKGIYVIGFYYPVVAKGQARIRVQISAGHEMGHLDKAIKA
                     FTEVGEKYGILHKNKDQIIEKYGL"
     gene            complement(6187..6975)
                     /locus_tag="PK717_01490"
     CDS             complement(6187..6975)
                     /locus_tag="PK717_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46375.1"
                     /translation="MGVVTKKNIFVFSSILLGIAFFFTIGIILGKSPLYLGNKMVDLS
                     GLAKMLPDCRIAQNKSSEDTIDVYFKCNPATEEEAALLSLSKARDVFQCVTKAGFNYS
                     NVRFILESGDFSEVKVFSKELVEGIFSGTVSSRSFWNKIYNLSPNPLNVPGISNKKQD
                     MIAFCAALGFSGLAIDENNNAKFDIKGQDWQKQFSRYCCALLAASKSTRCFLSGIEAS
                     IDGGTRKFFIKTDFKNISSLADGKISADELSRKIHMEWSNKTSK"
     gene            7092..8075
                     /locus_tag="PK717_01495"
     CDS             7092..8075
                     /locus_tag="PK717_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46376.1"
                     /translation="MKKLAGILAIVLLAGTMLSAIPTSAAPSLYGDLGRTFFFKPTAK
                     FKPSNDVKADNHVWFHFTNTDTRDHKFIGVITNLGPSDENNDWEIVQYCFSDACYQPL
                     VEGRTNRELKPGEKEDMSAQFAPHDKKSRGKFGMMGTARVDIWATDNKDLRDKMYIGE
                     VFIPFTRASMIINNKTMKLEKCLEWDPANKDRTKRYPLTVTDETLTVPPVLLGGSTFL
                     PFRSMGEKLLGAKVDWDAKNKVAMYTIGDDKGYFKMMLPINKKEATLILKCGDGTTLT
                     DKVALKVAPTIYQGSTVVPLRGVVELFGGYVEWTQAEKKATIILPPPEKEE"
     gene            complement(8162..8797)
                     /locus_tag="PK717_01500"
     CDS             complement(8162..8797)
                     /locus_tag="PK717_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46377.1"
                     /translation="MNEKLGPVYQALFNRFGPQHWWPGDSPWEIAVGAILTQQVAWKN
                     VEKAISALKESNLLDIDEMVGVDAEIIKELVRPVGFYNQKTARLIDFALYVKNRYGKI
                     EKMLDGETLKVRSELLSLKGIGRETADSILLYAGGHPIFVVDAYTRRLASCIGIDPDQ
                     DYDDLRAIFELGLERNPKLFNEYHALIVRLGKEHCRTKPLCEGCPVFPFRK"
     gene            complement(8814..9179)
                     /locus_tag="PK717_01505"
     CDS             complement(8814..9179)
                     /locus_tag="PK717_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46378.1"
                     /translation="MPAGDIVHLEFYTTDFEKFKKFYAPFNWKFTEWGGDYMLFMPPN
                     EQGVGGGIMKSQKPMPNQNMFIYILVGDINAELPKMKEIGWEIVEGRMPITGVGFFAV
                     LKDFDGNTINLFESLPRQP"
     gene            complement(9274..>9642)
                     /locus_tag="PK717_01510"
     CDS             complement(9274..>9642)
                     /locus_tag="PK717_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOR46379.1"
                     /translation="DCTPNMRVCQEEIFGPVATIIPFDTLEEAIQINNNTAFGLSSAI
                     ITNNVNNALVAARDITTGLVYINAGTIGAEVSTPFGGTRGTGNGHREGGVQVLDAFSE
                     WKTVTFDFSGHVQKAQIDNK"
BASE COUNT         2864 a         2208 c         2020 g         2550 t
ORIGIN      
        1 acactggaaa agcaatctcc agcgaaacag acaacatgac aaaaatgtgt ttgatggaca
       61 catacgattc tgaagaaatc gagaggcgct tggccactaa atgaacatag gcatcataag
      121 gatttcaacg aaggctgaca ggtaccgcct aaagcagaca attgagtcaa ttggcgttca
      181 ggtggtacag acgattgata gcgcaagcat gcccccaaca attgtttttg ttggcacaga
      241 agaccagatg ctcatggctt tcaagcaggc tgagaatctt ggggccgaat acgcaaaagt
      301 gttgacacac ataagggaag tgtcgcccaa gttggtaaaa accctaaaag aaggcccaaa
      361 attcaaaccg gggcaaccat tcggaattct tgaaactgcc ggcccagaag caatcttggc
      421 ttgtgcaaat ctcgcagcaa tgtttgacgg tatcgaactg gcagaaattc agctggcaag
      481 catgccaaac gagaaatcgc tcatggtcat aacgggtgat aagaaattga ttgaaaatgt
      541 tcttgagagt gcagaaatga tctgcgaaaa agcgcatttt acagtcccag gaagtaagcc
      601 ctggcaaaac gacggaaggc aaaactgaaa aatattttta ctccatggag aatggatttc
      661 atcaaatcca agaaacccga acactgtgtt ttctgcaagg caggcacaaa cataggtggc
      721 gaagataaag atttcatcgt ctatcgtgga gaaaaatgtt ttgtaatact caatcgctac
      781 ccctacgcaa caggccacat catgatcatt ccatacaggc actgtcaaaa gtttgaagat
      841 ctgggcaatg aagagctttc agaaatgatg atcttggcaa aaaaatttgt ttcagttcta
      901 aaacgtgcat tcacccccga tggattcaac atgggaatga atcttggtgc tgccgcaggc
      961 gctggtattg ctgaccatat ccacttgcat atagtgccaa ggtggatcgg ggacacaaat
     1021 ttcatgccca tttttgccga agttaaaatc caccccactg atttggaagg ggtttacaaa
     1081 gcaatcttgc aagcccttga agctaccgcc tgaaacttat cgcaagccca aacacaacaa
     1141 gggcataacc agttattctc acaagaacaa gcgcaacgct cagaatgctg ttttcgacta
     1201 ctggatatcc aaaaatattc tctccaaatc tcacggcgtg agacagtgca aaacataaaa
     1261 aggcaatact tataataaga aaagcccttg cgccatgttt tttatatgcc actatgccaa
     1321 gggaaacgat cgtgaggctg aatatgaggt ttactaagct ctcaatccat tgcggcatga
     1381 atattcaatc tcctttccgg cacggaacac atgagatggt gtcaccatcc ttgagatcaa
     1441 gtttgtttgc gtctatttgg gcaattacga ccttctcgtc aaggtttctc atcttgataa
     1501 cactaaatac aggtttgtaa taaatatttt tgaattctac cgagtcaatt ttttgtcccc
     1561 ttagtctcgc attccacata tcatacagat attcatcaac aaaaacctgg ccatgcctag
     1621 tattgccaac gcttattacc agttcttttt ggtgcgcgat tttctggata cccagggtgt
     1681 cataaaatct ggacatatca ataacttttg agtattcgtc gataactgaa attgcattct
     1741 tgaggcaaag gatgcacatc atatcagcaa aatcatcaat tgatagcgac atggactcaa
     1801 ataagtcaac aagcctggtg ccaattgtta tttttgaaag tatctcagat tcatttatac
     1861 caagtttggt tttggtaacc ctaggaacca ggaagatgtc ttttttgatt tctgcttttt
     1921 taatggctat cataccctcg tcaagcattt cattcacaag ttttttggtt tcttcctctg
     1981 aaaatgagag tgatgaaaca agattcgaat atgtaatccc actcccaatg agtcccgtga
     2041 tcagccattt atcgggattg atggctattt gaccgccttc cttgagtgga tttcttacaa
     2101 gcactgtgct ttgacaaatc ctgttttgaa gatttatata aactgacgcg gcctttaaaa
     2161 ggctttcagt gagagttttt atgttggttt caatggcata gccctgctcg aaagtgaact
     2221 taagtaagtc tagctttatt atctcataga atgcctgctc ggctattttt tcctgccatt
     2281 ttgcaaaagt tatttctcca gatctgacct gaacagaacc tgatccggaa gcatttgaca
     2341 ctgttattgt tccagtctgg ttttgtgaga gaaggaattt aaataaatcc agcaaaccag
     2401 tgccatttat ttcgccagat agcatgccaa taaggtatca taacggaaat tattgtcaat
     2461 ttctcaatat ttttataatt ttttaatatt ttggataaat atttacgaat gatccttcca
     2521 gaacatcgag cagtttttga tcatttggtc tgatgaaaaa agaatctctt ggcatctttc
     2581 ccttttccac aagaaaactg actttgccat ttgggcaaac tatttcaaca gaatcaattt
     2641 ttttgccaaa aagctggctt tcccacatcc caattacaaa ctcatcaata gccaatgtgt
     2701 taggtctgac acaaagtgag ttgtctattc taagcttcag caaaattggt ctggcaactt
     2761 tttcaaatcc aagcgattta taaatatacc acggtggcat tatcttcccg ttgtcatcag
     2821 acatgacaac catttttttg agaacgagcg aaacaagtat gtctgcaatg gatacagttt
     2881 ggttctcata ttgcgctaga aattcaccca gtgtcatccc atgtgctatt gaattaaaaa
     2941 cttgcttttc tctctcagac aggtctattg ggctctcttt tggaaacaag aaaagtgcat
     3001 ccaccataaa ttttggcttt atgaaaacta aaccttcttc aaccatcgag gagagtgttt
     3061 cctgaaggca tggcaaggag agttttgttg aaatggccaa ctgggcaacg ctaagtgggg
     3121 tgagtaaaga attcaaaatg ttttcttttt ggatggacaa tgaaacatta ggcaaaactc
     3181 tctcggtttt ttgcacaatt gttcttggaa ggaaacgttc atcaggagaa agtttgttta
     3241 tttttgataa atatttgaat ggtatgggtt tttcgcagtg ctgcttgttg agtctagtaa
     3301 aattgaatct cagcgatcca atttttgata acgccatgat cgcatctttg ccagttagat
     3361 cgccaaattt ggccatggca atattgcctt tatccacaac aatatcgcca ctgcatgaat
     3421 ctgtttcaaa aataatggtc cccgaatggc cctggttcaa aaaaaatgca agaagatcat
     3481 acaactctcc cccgacaatc tcgcctgagt gcatgacaaa tttatatcgt gattaagctt
     3541 gtgtgtaaat agaacattaa gggcctccaa ctatctattt gttgccatca aaatccaccc
     3601 aagtttgctt gacttgcgca cgttcaagca taatttattg gaggagtcga aggagaataa
     3661 gatacctccc ccggcaaaag cttaacggac ccacaaggag gactaaatga aagctgttca
     3721 gaaaactgaa ccaaaaatag gagctaaact tgttgaggtg ccaaccccaa ccaatctcaa
     3781 accaggatgg accctcgtca aagttagggc aacatccatt tgtggaacag atatccatat
     3841 ctggaactgg gaccaatggt cacaggggag aataggcgaa aaagctctgc cacagatact
     3901 cggacatgaa ttctgcggag aggtggtcga gttgggacca aactgcaaca ggataaaagt
     3961 tggcgattac gtatcagcag aaacacatat atatgatcca ggagacctta cctcaatgct
     4021 cgggatcttc cacgttggta acaacatgaa gattttgggt gttgatactg acggttgttt
     4081 tgcagattat gttgcaatcc cagaaatagt tctctggaaa aacgataaaa ccatgccacc
     4141 agaatacgca tctattcagg aaccccttgg caatgcctgc tatgctgtaa tgggcgagga
     4201 caatgacgtt tatggcaaaa caatgctcat aactggggat ggcccaatat cgctattcgc
     4261 aatcggcgtc gcaaaagctg ccggtgcgtc aaaaattatc cagtttggga tggcacaata
     4321 cagcatggag attgggcgca agatgggcgt cgatatacag atcgatgtca caaaaaccac
     4381 ccctgatgaa agatacaaga tagttatgga taacaccgga ggatggggtt gcgacattgt
     4441 ttgcgagatg ataggcgccc agagctctct tgatgacgct ttccgctgca tcagaaaagg
     4501 cggaaggttc tcggcattcg gcataccttc aaaatcacca tcacagatag actacaccga
     4561 tcagatcgtc tttaagggca tccagataca tggcatctcc ggaagaaaga tttttgacac
     4621 ctggtaccgc aatggaaaca tgctggcaaa ccgcaagctt gacccaacac cggttgtgac
     4681 ccatctaatg gcacttgagg attatcaaaa agcgtttgaa atgcttacag caccaacaag
     4741 gcagtgtgca aaaattgtca tgttccccga caaacaggaa ttgaatgcgg ccctggcccg
     4801 cagaaaataa ggaggaaaca aatggcatac ggaaagataa aagatttttt gacaaacgag
     4861 ctcaagggga taagggatgc cgggttatat aaagaagagc gcatcatagt gactccccag
     4921 gccgcagaca tcaaggtcca gtttccagag aacgcaccag tcaaagaagc actcaatttc
     4981 tgcgcaaaca actaccttgg actctcctcg catccagatg ttctaaaagc agcaaaagaa
     5041 ggcctggaaa tgagaggcta tggcctttcc tcggtacgct tcatctgcgg aacacaggac
     5101 atccacaaga aactggagag gaaactctcg gaatttcttg gagtcgaaga ttcgatactc
     5161 tattcatcat gttttgatgc aaatggtggt ctctttgagg ctatcatgaa agaagacgac
     5221 gccattatca cagaggaact caaccatgct tcgataattg atggcgtcag gctctcaaag
     5281 gcaaaaaggt acgtcttcaa acactccgac atgaatgacc tcgaggcaaa atgcaaggag
     5341 gccaggggcg ctggtgcaag acacatgata attgccaccg atggcgtctt ctccatggat
     5401 ggcgatattg ccaacctgaa gggtatctgt gatgttgcag acaaatatga tgccctcgtc
     5461 atggttgatg attcccatgc aaccggcttc attggaaaaa caggcagggg gacccatgag
     5521 cacaatggcg ttatgggaag gattgatgta ataacaacca ccctcggcaa agcgcttggt
     5581 ggtgcatccg gtggagcaac ctgtggaaga aaggaaatta ttgaaatcct ccgccagaga
     5641 tcaagaccat acctgttctc aaacaccatt ccaccagtcg ttgccagcgc agccctcagg
     5701 gtcatggaca tactctcgtc gacaaccgag cgcagggaca agctcgaaag aaataccatg
     5761 tatttcaggg aaaaaatgac atctgccggt tttgacataa aaccaggtgt tcacccaatt
     5821 gttccaatca tgctctacaa tgcaaagctc agccaggaca tggcaaaaga cctctatgaa
     5881 aagggtatat acgtcatagg gttctactac cctgtcgtgg ccaagggaca ggcaagaata
     5941 agggtccaga tttcagcagg acacgaaatg gggcaccttg acaaggcgat aaaggccttc
     6001 acagaggttg gagaaaaata cggcatcctc cacaaaaaca aagaccagat aatcgaaaaa
     6061 tacgggttgt aatcggcata atccccacaa aaaaaaggcc accctcatgg tggccttttt
     6121 tttggtttca acatcatgtt ttttgttatt tgatcacaac tttttcagca gctctctttt
     6181 tgttttttac ttgcttgttt tgttgctcca ttccatgtgt atttttctgg ataactcatc
     6241 tgcagaaatt ttgccatcag ccaaactcga aatatttttg aaatctgttt ttataaaaaa
     6301 ttttctcgtt ccaccatcaa tgcttgcctc aataccagac aaaaaacatc tcgtgctttt
     6361 cgaggcagcc agaagcgcgc aacaatacct ggaaaactgt ttttgccagt cctggccctt
     6421 tatatcaaat ttggcattgt tgttctcatc aattgccaac cccgaaaacc ccagtgccgc
     6481 gcaaaaagca atcatgtcct gctttttatt ggagatacca ggaacattca aagggtttgg
     6541 agacaaattg tatatcttgt tccagaaaga cctggagctc actgttcccg aaaaaatccc
     6601 ttccacaagc tctttcgaaa aaacctttac ctcgctaaaa tcacccgact ccaaaataaa
     6661 tctcacgttg gagtaattaa agccagcctt tgtgacgcac tgaaaaacat ccctggcttt
     6721 tgacaaggaa agaagggccg cctcttcctc cgttgcagga ttgcacttga aatacacatc
     6781 gatggtatct tcactggatt tgttctgggc tattctgcaa tctggaagca ttttcgcaag
     6841 ccctgataaa tcaaccattt tgttgccaag atacagcggc gatttcccta atattatccc
     6901 aattgtgaaa aaaaatgcta tcccaagaag aattgacgag aaaacaaaaa tattcttttt
     6961 ggttactacc cccacttgta cctccctcca gaacctgcgg ctcttttttg ccttgtcttt
     7021 gttttacctt tattgtggat ttcacaattg gttatagtat aggttataca aaatttctag
     7081 gaggtaggaa aatgaagaaa ttggcaggaa ttctcgcgat tgttcttctt gcagggacaa
     7141 tgctctcagc aataccgact tccgccgccc cgtcactgta cggagacctc ggcaggacat
     7201 tcttttttaa gccaacagca aaatttaaac caagcaacga tgtcaaagct gacaaccatg
     7261 tctggttcca ttttacaaac accgacacca gagaccacaa gtttattggc gtcataacca
     7321 accttggacc atcagatgag aacaacgatt gggaaatagt ccagtactgt ttctctgatg
     7381 cttgctatca accattggtc gagggcagga caaacaggga actcaaacca ggcgaaaaag
     7441 aggacatgtc agcccagttt gctccgcacg acaaaaaatc caggggcaaa ttcggcatga
     7501 tgggaaccgc aagggttgac atctgggcaa cagacaacaa agatcttcgc gacaagatgt
     7561 acatcggcga agtattcatc ccgttcacca gagcctcgat gatcatcaac aacaagacaa
     7621 tgaagcttga gaaatgcctt gagtgggacc cagccaacaa agacaggacc aagaggtatc
     7681 cgctcacagt gaccgatgaa acacttactg ttccaccagt tcttcttggt ggctcaacat
     7741 tcctcccgtt ccgttcaatg ggcgaaaagc tcctcggtgc aaaggtcgac tgggacgcaa
     7801 agaacaaggt tgcaatgtat accatcggcg atgacaaggg atacttcaag atgatgcttc
     7861 caatcaacaa gaaagaagcc accctcatcc tcaagtgcgg cgacggcacc accctcaccg
     7921 acaaagtcgc cctcaaggtt gccccaacaa tttaccaagg cagcaccgtt gtcccgctca
     7981 ggggcgtagt tgagctcttt ggtggatatg ttgagtggac ccaggctgaa aaaaaggcca
     8041 caatcattct cccgccaccg gaaaaagaag aataatataa ctcttcaaaa ctaccatcat
     8101 cgtatataaa aagccggccc tttggggccg gcttttttgt tgttgtactc agaaatttca
     8161 gctacttcct gaaaggaaaa acagggcatc cctcacacaa tggttttgtc ctgcaatgct
     8221 ccttcccaag tcttacgatt aatgcgtgat attcattaaa cagttttggg tttctctcaa
     8281 gccctaattc aaaaatggcc ctcaaatcgt catagtcctg gtcagggtca ataccaatgc
     8341 agcttgcaag ccttctcgtg taggcgtcaa caacaaaaat ggggtggcca ccagcgtaaa
     8401 gaaggatcga gtccgcagtc tccctgccta tgcccttgag tgacagcaat tcgctcctga
     8461 ccttgagcgt ctcgccatcc agcatctttt ctattttgcc gtagcggttc tttacataaa
     8521 gcgcaaaatc aatcaatctg gcagttttct gattataaaa cccaacgggt ctgacaagct
     8581 cttttatgat ctctgcatcg acgccgacca tctcatcgat atcaagaaga ttactttctt
     8641 tcaaggcaga aattgccttc tccacatttt tccaggccac ctgttgtgtg agtattgcac
     8701 caacggcaat ctcccacggt gaatcccccg gccaccagtg ctgcgggcca aatctgttaa
     8761 aaagggcctg ataaacaggc ccaagttttt cattcataat atctggtttt tgtctaaggt
     8821 tgccttggca agctctcgaa aagattgatg gtgttgccat caaaatcttt caggacagca
     8881 aaaaatccca cgccggtgat tggcatcctg ccttccacaa tttcccagcc aatctccttc
     8941 atcttcggca attctgcatt gatgtcacca acaaggatgt agatgaacat gttctggttt
     9001 ggcattggtt tttgcgattt catgattccc ccgccaacac cctgctcgtt tggcggcatg
     9061 aaaagcatgt agtctccacc ccattcagta aacttccagt tgaatggtgc atagaacttc
     9121 ttgaattttt caaaatcggt cgtgtaaaac tccagatgta ctatatctcc ggctggcatt
     9181 agatacctcc ttttcaaatt tagaaaatag tgattttcaa aactgaaact gattctaata
     9241 aaaaagggtg cccctagggg caccctcata tttctacttg ttgtctatct gcgccttctg
     9301 gacgtgtccg gaaaaatcaa aagtgaccgt tttccactct gagaaggcat ccagcacctg
     9361 cacgccaccc tcacgatgac cgttgcctgt tcccctcgtt ccaccaaatg gtgtcgagac
     9421 ctccgcgccg attgtcccgg catttatgta aacaagacca gtggtgatgt cccttgcggc
     9481 aaccagtgca ttgttcacat tgtttgtgat tattgccgag gagaggccaa aagctgtgtt
     9541 gttgttgatc tggattgcct cttcaagcgt gtcgaacggg attattgtgg caaccggtcc
     9601 aaaaatctct tcctggcaga ctctcatatt tggagtgcaa tc
//
LOCUS       DAOSRI010000028         9616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_6870_length_9616_cov_4.142977, whole genome shotgun sequence.
ACCESSION   DAOSRI010000028 DAOSRI010000000
VERSION     DAOSRI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_9616_cov_4.142977"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="PK717_01515"
     CDS             <1..>1082
                     /locus_tag="PK717_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR46380.1"
                     /translation="LTGKDVLVGIVDSGISYDHPSLSGGFNPDSKVCVGYDFANMDDD
                     PMPEPGHVGWHGTHCAGIVAGSGLPGVPSSDPAPSGIAPDAKLGAYKVFGKSGGARND
                     RVIAAIERAYKDGCKVISLSLGSNYVWADEPVCKLIDRVVENGVVVVAAAGNDGDLSR
                     DELPYKIISPGGAKNAICVGAVDDSQYPHLVAGNVDCLPEYLTYSKPIDDPITGPLVF
                     CGLGKTDDVKDIDLTGKIALIKRGDNTFFDKATNAQSKGALACVIFNNEEGDVQGTLG
                     SSGINIPVVGISSDIGDKLNGFASTGNNATLDVKSKLGLMAGFSSQGPTNDFRLKPDV
                     SAPGVNVLSAVGSNSYVRMSGTSMATP"
     assembly_gap    1083..1092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1093..2549
                     /locus_tag="PK717_01520"
     CDS             <1093..2549
                     /locus_tag="PK717_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46381.1"
                     /translation="SGACAILIQAHPEWTPFDVRSAIINYAVPQKDKEGNLHPVFSQG
                     TGRIDIVSSIGAKALFRPTSVDLGYIKSDSTASVTIRNITKAPLKVTVTNEPEGGQSA
                     KIGEITLKASESKLFTTNIAPDTSTAGAHAGYLVFDYGDSKARVAYLYFTGDKPDPPT
                     VSDIKMPLPTFSPNGDRRGDYLSATVSLNKMVDGFELDLTDTNNKILTILDYSYGTQG
                     GGMWDLGWDGSIEGMKIQDGIYRIHLYTLVTGRDPKEQANWEHFGPIEFMATTKAPLI
                     QLNATNGKFYNLDGPVTLSGSIKDPLFDFQGLGEKAFEMVVLNSEKPLEIKEDGSFSF
                     QTTLKRGDNSVSITVLNIAGLKTVLTINLQSLRKAEISWDNTGITLNGKKMPDVSFTI
                     VDNEIMVDASKLGLLADGITFDKKGQNLTFTLDKKAVLMMIGQPFIIVDGKASFCNPP
                     EAKDNVVMIPLGALVTAFGGDVGDSRATIVWRGD"
     gene            2546..3244
                     /locus_tag="PK717_01525"
     CDS             2546..3244
                     /locus_tag="PK717_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46382.1"
                     /translation="MNWIDYIFLAFVLYMAVKGFIIRFSGAILGSLATLIAWIAAFLL
                     AAPVKNLLQSLFGVVSWLASLLSPAIPGTPLQGCSTVADALVKCGLPPWAKGMLGRVV
                     DQGYVVSTTGDLISYWVANTIIVVIVFMLLLMIIGFFTRMLVERTKLSLPQEGFVHDF
                     DNLLGGATYALLAFFIGFGILAIFAALFPMGVAGKSPVGTYVSQSFFGGLVYNNFLGI
                     QTIYGSITRLVLGY"
     gene            3234..4586
                     /locus_tag="PK717_01530"
     CDS             3234..4586
                     /locus_tag="PK717_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOR46383.1"
                     /translation="MAISLTLPFTISASKEPAIRVGLGSIAEEFTIGGTDQFSVVEAS
                     SGKVACTLQKETKCLVQDGEKAVYSVFIDTFYTSTDAENFAKTLLSRFPAGTSYNIVS
                     QKGVFSLEIGEYPTEDEAKNAIKSFLSDLPKAAVVAHAGLTVKIGDNIVMHPIVTGKA
                     TKIFAIEIKEGQYAKYNSRRYRGILELFYSNEKFVMVNTLPFEDYLRGVVPAEMPALW
                     HIEAVKSQTVAARTYAIRYIDRPTNYAYDICADTGCQVYGGLDREQPQSNKAIDETRG
                     MVAIYQGKPIDAVFFANSGGYTENSEYVFSSPTPYLKGVPSPGEEESPRHTWFKTVPM
                     EEFQEIFKPLVKTDIGEIIDWKPTKLGVSGRTYAGSAIGTKGEAKVSGGTVRSAFSLN
                     STWFDVVFASGFVVFCGRGWGHGVGLSQYGAKAMAQSGKTYTQIISHYYQGALLTKWY
                     "
     gene            4708..6204
                     /locus_tag="PK717_01535"
     CDS             4708..6204
                     /locus_tag="PK717_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46384.1"
                     /translation="MKKLATFALLFFLVLGGFYGTASSQDMATLWISPQLECVTVGSE
                     ILVQVKITECPFAYGIGAQILFDTQYLEINPDKVVEGEFFRQDGATTYFASKVTTDPQ
                     NPKVGKLIFGITRFNDQNDSYGPIAGEGLVLYFSAKVKVVGECALGFKEVNLINLQTK
                     QGETSLEKVPVKLEESKINICAKDNIAPNVTITKSPPLETNQVIGQFCYQATDDSTPQ
                     DKIQYSVRLEDPIRGGEWTPWMDKTCYSYTFGAQGKNTFCVRARDEFGNVSQPACVTI
                     NYDSTPPILELAPIPPEVEEITYNLCGKTEKPETGISLLVQGARVPINNDGTFCVQLR
                     LVEGPNTVHVAAIDKAGNIVQETHSVSLIKKTTIIMNEGQRQGTINGSVVIIDPPPTN
                     IKGRVLVPLRFITEAFGMNPTYESTEKRVDIIWTVKEGGTPIQHHLRLWINKKNYTLD
                     GKTMFLDVPPQIVSGRTMVPIRFIAESFGATIDYDSANRRVTIVYVKR"
     gene            6216..>9616
                     /locus_tag="PK717_01540"
     CDS             6216..>9616
                     /locus_tag="PK717_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46385.1"
                     /translation="MKKILVLLLSVAIMAPLVPFPATIQSTIAADGCFDTRKGNNFRD
                     GVYQGTQINAPALLQTWYWQSIANISTPPVACGSRVYIASNDRKVHCIDRTTGEELWE
                     TTEMSNFPVSSPTINVGTQNLYVATGGQIERQSVLYCFDAVTGTQRWQWTPGSEGSTA
                     QITSGPTVVGENDGLVPGQMMVLVTVDKGAIFALDYKTGTRIWGFGFNGFPQSDPVVA
                     EQKIFQLNTLGVLTILDLKTGKLISQTVIDDKEAYEFNNTPCYIPSYNLLICASRGKS
                     VLGAKGKIYGINTTTGNVIWGKNMEIDQFHRAGPSVVNPPVVENTGILIGSDTGTVYM
                     IDPPTGRMRWSYKLGMPITTDIIVSGKYAVLGAANMLYAIDAAKGNVVFKQSLGNTIT
                     GHPIVNEGMVYCACNDARLYAFSDHDDFTIDALPRSEGIYPGNKRSFKVTISATMNFS
                     HPLTLSAVGLPTTLKAQFSKPFIFPGIEPVEVDLIIDASPEAQPGNYICNVSGMLLGR
                     ERNAIIQIQVLPPLAGDFKLSVSSDQLSPDRNVNQGDSISFQVKVEAIGGFNAEVAFY
                     PNQSTLPPGMQVYFIPDKITPDGYATAVIRTDTTTQADRYVIELQGHAGGKVRTTNVW
                     FTVGGFDTEDWTQFQQNIFKTGANSKEKFLTDPEPRWDFSFKPDDNPEAVVRYRTQPI
                     NGMGKLFVVAEWESKDNSRIHRSSLICLDAVLGTKAWSFDFGKSVSEVDNFDDNEDKQ
                     WPVMCTPALDTENGYIYIGSLDGNFYCINTKTGQRVWSYNTTKPIRTSVLLLKQPEVP
                     TKRVYFATVNGTVYCMAAHTDIDTIIWKQQLPASCYAPFSYAFNRVPQVNKGTVLVPC
                     MDGKVYELDAQDGTKYWASEFFKSQPINACPVDVDKGEFYFAGAQGATDDNYCYGSSM
                     IYRNNISDGSMRWQLATFGPTFGSPALRINPDKDVVHVNANWGITPEIRTQTNRFVRV
                     ESENRQKILDNVIGEWSCEKRQYSYGSVIIDKDAQSMVYNKDGQIYCFDPNGKTVFTI
                     KTNRPSKSHLTFSRRMLYYATEDGHVMAWAQKWGFGLAPETGSPVICQGTSMPLNIFF
                     RSEIPLKTAVRFKIIQAPPDTTTTFSPDTLSASGTTTLSISVGEG"
BASE COUNT         2729 a         2409 c         2329 g         2139 t
ORIGIN      
        1 ttaaccggca aggatgtgct cgttggcatt gtcgattccg gcataagcta cgaccaccca
       61 tcgctttcag ggggattcaa ccctgactca aaagtttgtg ttggctacga ttttgcaaac
      121 atggatgatg atccaatgcc agaaccagga catgttggct ggcatggaac acactgtgca
      181 ggaattgtcg ctggttcagg gctccccgga gttcccagtt cagacccagc cccaagcgga
      241 atagcacccg atgcaaaact tggcgcctac aaagtctttg gcaaaagtgg cggggcaagg
      301 aacgacaggg taatagccgc aatagagagg gcctacaagg acggatgcaa ggtcatatcc
      361 ctgagccttg gaagcaacta tgtttgggcc gatgagcccg tttgcaagct gatagacagg
      421 gtggtcgaaa atggagttgt cgttgtggcc gcggccggga atgatggtga cctgtcaaga
      481 gacgagctcc catacaagat aatctcacca ggcggagcaa aaaatgcaat atgcgtcggg
      541 gctgttgatg attcgcaata cccgcacctg gtggctggca atgttgattg tttaccagaa
      601 tacctcacct attcaaaacc catagatgac ccaatcacag gcccactggt tttttgcggc
      661 cttggaaaga ctgatgatgt aaaagatatt gatcttactg gcaaaattgc cctcatcaaa
      721 aggggcgaca acacattttt tgacaaggcg accaatgcac aatcaaaagg ggctttggcc
      781 tgcgtcattt tcaacaatga ggagggagat gttcagggaa cacttggttc ttcaggcatt
      841 aatatccccg ttgttggtat atcaagcgat ataggcgata agctgaatgg gtttgcctcc
      901 accggcaata acgcaaccct ggatgtcaag agcaagcttg gcctgatggc tggcttttcc
      961 tcacagggcc cgacaaacga tttcaggcta aaacctgatg tttctgcacc tggtgtcaac
     1021 gtcctttcgg ctgtcggcag caacagctat gtaagaatgt ccgggacaag catggccact
     1081 ccnnnnnnnn nntttccggc gcatgcgcca tactcatcca ggcccatccc gaatggacac
     1141 cgtttgatgt gaggtcggca ataataaact atgctgttcc acaaaaagac aaagaaggga
     1201 atttgcaccc ggtcttctca cagggcaccg gaaggatcga cattgtttca tccataggcg
     1261 cgaaggcgct cttccgcccg acatcagttg accttggata cataaaatct gactccactg
     1321 catcggtaac aatcagaaac atcacaaaag ccccgctcaa agtaacagtg accaacgagc
     1381 cagagggcgg gcaatcagca aaaattggcg aaatcacact caaagcgtcc gaatccaagc
     1441 ttttcacaac caacatcgcg ccagacacat caacggctgg ggcgcacgca ggttatctcg
     1501 ttttcgacta tggcgactca aaagccaggg tggcctacct ctacttcacg ggagacaaac
     1561 ccgatccccc aacggtttct gacataaaga tgcctttgcc gaccttctcg cccaatggcg
     1621 acagaagggg tgattatctg agtgcaacgg tgtccctgaa caaaatggtg gacggtttcg
     1681 agctggatct aactgataca aacaacaaaa ttttgaccat ccttgattat tcctacggga
     1741 cccagggtgg tggaatgtgg gacctcggct gggacggcag tatagaagga atgaaaatcc
     1801 aggacggaat ttacaggata cacctctaca ccctcgtaac cggaagagac ccaaaggaac
     1861 aggcaaactg ggaacacttt gggccaatcg aattcatggc cacaacaaaa gccccattga
     1921 tccagctgaa tgcaacaaat gggaaattct acaatcttga tggacctgtg accctttctg
     1981 gctcaatcaa ggacccgctc tttgatttcc aaggtcttgg cgaaaaggcc tttgagatgg
     2041 ttgttttaaa ctctgagaaa ccattggaaa taaaggaaga tggcagcttt agtttccaaa
     2101 ccacgctcaa gaggggcgac aactctgttt caataactgt cctgaacata gcaggcttga
     2161 aaacagtcct tacaattaac ctccagtctc tcagaaaagc agaaatttcc tgggacaaca
     2221 ccggcatcac cttaaacggc aaaaagatgc ctgatgtttc gtttacaatt gtcgacaatg
     2281 agataatggt tgatgcttca aaacttggcc tccttgctga tgggatcaca tttgacaaaa
     2341 aagggcagaa cctgactttt acactcgata agaaggctgt cctgatgatg attggacaac
     2401 catttattat agtggatggc aaagcgtcat tctgcaatcc gcctgaagca aaagacaatg
     2461 tggtaatgat tcctcttgga gcgttggtaa ccgcttttgg cggagacgtt ggagattcaa
     2521 gagcaacaat tgtctggagg ggtgattgaa ctggattgat tacatcttcc tagcttttgt
     2581 cctgtacatg gccgtcaagg ggttcattat aagattttcc ggtgcaatcc tcggtagtct
     2641 tgcaacactg attgcgtgga ttgcggcatt tttgctggcc gctccagtaa agaatctcct
     2701 ccagtcattg tttggtgtcg tctcgtggct ggcatcactg ctctcccctg ccataccggg
     2761 aacaccactt cagggctgtt caactgttgc agatgcactg gttaaatgcg gtcttccgcc
     2821 atgggcaaag ggaatgctgg gaagggttgt tgaccaggga tatgtggttt caacaactgg
     2881 cgacctcatc tcttactggg tcgcaaacac gataatcgta gtgatagtat tcatgctatt
     2941 gctcatgata atcggatttt ttacaaggat gctggttgaa agaaccaaac tctcacttcc
     3001 gcaggagggt tttgtccacg atttcgacaa cctccttggc ggagcaacct atgcccttct
     3061 cgcctttttt atcgggtttg gcatcctggc catttttgca gcacttttcc ccatgggcgt
     3121 tgctggcaag tcgcctgtgg gaacttatgt ttcacaatca ttcttcggag gtctggtcta
     3181 taacaacttc ttgggaattc aaacaattta cggctcaatc actcgccttg tgcttggcta
     3241 ttagcctaac gctccccttc accatatcgg caagcaaaga accagccata agggttgggc
     3301 tggggtcaat tgccgaagaa ttcaccattg gcggaacaga ccagttctct gttgtcgaag
     3361 catccagcgg gaaggtagca tgcactctcc agaaggaaac aaaatgcctc gtccaggacg
     3421 gtgaaaaagc agtttattca gtattcatag acactttcta caccagcacg gacgctgaga
     3481 actttgccaa aacactgctt tcgaggttcc cggctggtac tagctacaat atcgtttcgc
     3541 aaaagggtgt tttttcactc gagattggcg aatacccgac agaggacgag gcaaaaaacg
     3601 ccataaaatc tttcctctcc gatctcccaa aagcagcggt cgttgcgcat gctggtttga
     3661 cagtaaagat cggcgacaac atcgtcatgc accctatcgt gaccggaaag gccacaaaaa
     3721 tatttgcgat tgaaataaaa gaggggcaat acgcaaaata caacagcaga aggtaccggg
     3781 gaatacttga gctgttttat tcgaacgaaa aatttgtaat ggtcaacact cttccgtttg
     3841 aagactacct gaggggtgtt gttcctgcag agatgccggc actatggcac attgaagcgg
     3901 tcaaatcgca gaccgttgcg gccagaacat acgcaatacg ctacatcgac aggccaacaa
     3961 actacgccta cgacatctgt gcagacaccg gttgtcaggt gtacggcggt ctggatcgtg
     4021 agcaaccgca atcaaacaaa gcaattgatg aaacaagggg gatggttgcc atctaccagg
     4081 gaaaaccaat agatgcagta ttttttgcca actctggcgg ttacacggaa aattccgaat
     4141 acgtcttttc gagcccgacc ccgtatctga aaggtgtccc ttcgccaggc gaggaggaat
     4201 cgccgcgcca cacatggttt aagacggtgc ccatggagga gttccaggag atattcaaac
     4261 cgctggtcaa aacagacata ggggaaataa tcgactggaa accaacaaaa ctgggtgtct
     4321 ccggaaggac ctatgcaggg tctgccatcg gtacaaaagg tgaagccaag gtttcaggtg
     4381 gaaccgtaag gtcggcattt tcactcaaca gtacctggtt tgatgtggtt tttgcttcag
     4441 gatttgttgt tttttgcgga agaggatggg gccacggcgt cggactctcc cagtacggtg
     4501 ccaaggccat ggcgcaatcc gggaaaacgt atacccagat tatatcccac tactatcagg
     4561 gggcgttact gacaaaatgg tattaaaccg catatctgat gaaacgtatt taagtttgac
     4621 cggcaaaaga gtggtattta taaaccaata tgtatttttt ctttccgtaa taggttttca
     4681 aaaccaagtg tttgggaagg aggaattatg aaaaaactgg caacgtttgc attgctgttt
     4741 tttcttgttt tgggtggatt ctatgggaca gcatcgtctc aggacatggc gaccctgtgg
     4801 atatcacccc agcttgagtg tgtaacagta ggctctgaga tcctcgtgca ggtgaagata
     4861 actgaatgtc ccttcgccta cgggatcggt gcccaaatac tttttgacac ccaatatctc
     4921 gagatcaacc ctgacaaggt ggttgagggc gaattcttca ggcaagatgg cgccacaaca
     4981 tattttgcca gcaaggtgac cactgacccg caaaacccaa aggtcggcaa gctgatattt
     5041 ggcattacca gattcaatga ccagaacgat tcatacgggc caatagctgg agagggtctc
     5101 gtcttgtatt tctcggcaaa ggtgaaagtg gttggcgagt gcgcgctcgg cttcaaggaa
     5161 gtcaatctga tcaacctcca gacaaaacag ggcgaaacct ctctcgaaaa agtcccggtg
     5221 aaactggaag aatcaaaaat aaatatctgt gcaaaagaca atatcgcccc gaacgtgacc
     5281 ataaccaaaa gcccacctct agaaacaaac caggtcatag gacaattctg ctaccaggcg
     5341 acagatgaca gcacccctca ggacaaaatc cagtactcgg taaggcttga agacccgatc
     5401 aggggtggcg aatggacacc ttggatggac aagacctgct attcctacac gtttggtgcc
     5461 cagggcaaaa acaccttctg cgtcagggca agggacgagt ttggaaacgt ctcccaacct
     5521 gcatgtgtga caataaatta tgactcgacc ccgccaatcc ttgagcttgc cccaatacct
     5581 ccagaagttg aagaaatcac atacaatctc tgcggcaaaa cggaaaaacc ggaaaccgga
     5641 atctcgctcc tcgtgcaggg tgcaagggta ccaataaaca atgatggtac cttctgtgtc
     5701 cagctcaggc tcgtggaagg cccgaatacc gttcatgttg cggcaatcga caaggccggc
     5761 aacatagtcc aggaaacaca ttccgtgtcg ctcatcaaga agactactat cataatgaat
     5821 gaaggccagc gccagggaac aatcaacgga agcgtcgtca taattgaccc accaccaaca
     5881 aacatcaaag gccgtgttct ggtcccacta agattcatca ctgaggcctt cggcatgaac
     5941 ccaacttacg agtcaacaga aaaacgtgtc gacattatct ggacagtcaa agagggtggg
     6001 acaccgatac agcaccacct gaggctctgg ataaacaaaa agaactacac gcttgatgga
     6061 aaaacaatgt tccttgacgt accaccacaa attgtttccg gaagaacaat ggttccaata
     6121 aggttcattg cagaatcttt tggtgcgaca atagactacg actcggcaaa ccgcagggtc
     6181 acgatagtat acgtgaagag ataaaggagg aaagcatgaa gaagatccta gttttgctgc
     6241 tttctgtggc cataatggca ccacttgttc cgttccccgc aaccattcag agtaccatcg
     6301 ccgcggacgg atgttttgat acaagaaaag gaaacaactt cagggatggc gtctaccagg
     6361 gcacccagat aaatgcaccc gcccttctgc aaacatggta ttggcagagc atagccaata
     6421 tctcgacacc ccctgttgct tgcggatcaa gggtctatat cgcatcaaac gaccgcaagg
     6481 tgcactgcat cgacaggacg accggcgaag agctctggga gacgacagaa atgtcaaatt
     6541 tcccggtttc atccccaaca atcaatgtcg gcacccagaa cctctatgtg gcaaccggcg
     6601 gacagattga acgccagtca gtgctttatt gctttgatgc agtaacaggc acccagagat
     6661 ggcaatggac gccaggctcg gagggaagca cagcacaaat aacatcaggc cctacagttg
     6721 tcggtgaaaa tgatggcctg gtgccagggc agatgatggt ccttgtgacc gtggacaaag
     6781 gcgcgatctt cgcccttgac tacaagacag gaacaaggat atgggggttt ggtttcaacg
     6841 gcttcccaca atctgatcct gttgttgcag agcaaaaaat cttccagctc aacaccctgg
     6901 gtgttttgac aatacttgac ctcaagaccg gaaagctcat ctcccagaca gtcatcgacg
     6961 acaaagaagc atatgagttc aacaacaccc cgtgctacat ccccagctac aatctcctca
     7021 tttgcgcctc aaggggcaaa tcagtgctcg gtgcaaaggg aaaaatctat ggaatcaaca
     7081 caacgactgg caacgtcatc tggggcaaga acatggagat tgaccaattc cacagagcag
     7141 gtccctctgt cgtgaaccca ccagtagtag aaaacactgg aatcctcatt ggatcagaca
     7201 ccggcaccgt ttatatgata gacccaccaa ccggaaggat gcgctggtca tacaaacttg
     7261 gaatgcccat caccactgac atcatagtct ctggaaaata cgcagtcctt ggcgcggcaa
     7321 acatgcttta tgcaattgat gccgcaaagg gcaatgttgt attcaagcag tcgcttggaa
     7381 acaccataac cgggcacccg atcgtcaacg agggcatggt ttattgcgcc tgcaatgacg
     7441 ctaggttgta tgccttctcg gatcatgatg atttcacaat agacgcactc ccgaggtcag
     7501 aggggatata cccaggcaac aagagatcat tcaaggtaac tatttcggcc acaatgaact
     7561 tctctcaccc gctcacacta tctgcggttg gtttgccaac caccctgaag gcccagttct
     7621 caaaaccatt cattttccct ggaatcgaac cagttgaagt tgatcttata attgatgcat
     7681 caccagaagc acagccagga aactacattt gcaacgtaag cggaatgttg cttggcaggg
     7741 aaagaaacgc cattatccag atccaggtcc ttccgccact ggcaggcgac ttcaagctgt
     7801 cagtgtcatc ggaccagctc tctcctgaca gaaacgtcaa ccagggagac tcgatatcgt
     7861 tccaggtgaa ggtcgaggct atcggcggat tcaatgcaga ggtggccttc tatcccaatc
     7921 agtcgacact cccgccgggc atgcaggtct acttcattcc ggacaagata acacccgatg
     7981 gctacgcaac tgcggtcatc aggacagaca ccaccacaca ggcagacaga tatgtaatcg
     8041 aactgcaggg tcacgccggc ggaaaggtga ggaccacaaa cgtatggttt actgttggcg
     8101 gatttgacac cgaagactgg acgcagttcc agcaaaacat cttcaaaaca ggcgccaatt
     8161 ccaaagaaaa attccttact gatccggaac cacgctggga cttcagcttc aaaccggatg
     8221 acaacccgga ggccgtagtc agatatcgca cacagccaat aaacggtatg ggcaagctgt
     8281 tcgttgttgc agaatgggaa tcaaaggaca actcgagaat acacagatct tcgctgatct
     8341 gccttgatgc tgttctggga acaaaagcgt ggtccttcga tttcggaaag tctgtctcgg
     8401 aagtcgacaa cttcgatgac aacgaagaca agcagtggcc agtcatgtgc accccggcac
     8461 tcgacaccga aaacggctac atatacatcg gctctctcga cgggaacttc tattgcatca
     8521 acacaaagac cggccagagg gtgtggagct acaacacgac aaaaccaatc agaacgtctg
     8581 tgctcctact caaacagcca gaggtaccaa caaagagggt atatttcgca acagtcaacg
     8641 gcacggtcta ctgtatggca gcccacaccg atattgacac aataatatgg aagcagcagc
     8701 tcccggcatc ctgctacgcc ccattctcct atgcgttcaa cagggtcccg caagtaaata
     8761 agggaacggt ccttgtccca tgcatggatg gcaaggttta tgagcttgac gcccaggatg
     8821 ggaccaagta ctgggcatca gaattcttca agtcccaacc aatcaacgct tgccccgtcg
     8881 atgttgacaa aggcgaattc tactttgcag gtgcccaggg cgccacggac gacaactact
     8941 gctacggcag ctcgatgata tacaggaaca atatatctga cggctcgatg cgttggcaac
     9001 tggcaacctt cggaccaact tttggctcgc cggcactcag gatcaacccg gacaaggatg
     9061 ttgtccatgt caacgcaaac tggggtatca ctccagaaat aaggacacag acaaacaggt
     9121 tcgtgagggt cgaatcggaa aacaggcaga aaatcctcga caatgtcatc ggcgaatgga
     9181 gctgtgaaaa gagacagtat tcctatggtt ccgtgataat cgacaaagac gcccagtcca
     9241 tggtctacaa caaggacggc cagatatact gctttgatcc aaacgggaaa actgttttta
     9301 ccatcaaaac aaacaggcca agcaagtccc atctgacatt ctccagaagg atgctgtatt
     9361 atgcaaccga agacggccat gtcatggcct gggcccagaa gtggggattt ggtcttgccc
     9421 cagagacagg cagtccggtc atttgccagg gaacatcaat gccgctcaac atattcttca
     9481 gatcagaaat cccgctcaag actgcggtca ggttcaaaat aatccaggca ccaccagaca
     9541 caacaacaac attcagccct gacaccctgt cagcttccgg aaccacaaca ctgtcgataa
     9601 gcgtcggtga aggttg
//
LOCUS       DAOSRI010000029         9349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7082_length_9349_cov_3.911556, whole genome shotgun sequence.
ACCESSION   DAOSRI010000029 DAOSRI010000000
VERSION     DAOSRI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_9349_cov_3.911556"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PK717_01545"
     CDS             complement(<1..1079)
                     /locus_tag="PK717_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46386.1"
                     /translation="MGKNYPKAEKGNVVDDYFGVKVADPYRWLEDENSEETKNWVEAQ
                     REFFSNEIEKMPFRDKIRQRITELTAYKLPFPPFLHSFGHTSIVKNGKLYFTFSGTQK
                     IMAMDLIKRKPYEAIKYDIDPDRQYVGISSFSPDGSKAAISFKEDGSDWTDIMVADLE
                     KKEFLPERLTKVRYAGTTWLDGSGFYYTRFDEVIEEKRLTQKLTRPKLYYHKVGTGQE
                     QDVLVLKSQIDLVMLACSDIIDEKYLLINEYGEINDYYLQEIDYPKNKIPIIENNTDQ
                     FSYMGVIGKQLIFSTNIDCPMGKVVMIDPANPKKENWQTIIHDPGACMENAVLSYGKI
                     LATFKNGPKNYARQYDLQGNFEHEI"
     gene            1270..1346
                     /locus_tag="PK717_01550"
     tRNA            1270..1346
                     /locus_tag="PK717_01550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Met,seq:cat)
     gene            1354..1429
                     /locus_tag="PK717_01555"
     tRNA            1354..1429
                     /locus_tag="PK717_01555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1387..1389,aa:Thr,seq:ggt)
     gene            1481..2686
                     /gene="tuf"
                     /locus_tag="PK717_01560"
     CDS             1481..2686
                     /gene="tuf"
                     /locus_tag="PK717_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR46387.1"
                     /translation="MAKQKYERTKPHVNVGTIGHIDHGKTTLTSAITKTLATLGHTVA
                     KAYDEIDNAPEEKARGVTINIHHTEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVAASEGPMPQTREHILLARQVNVPRIIVFLNKIDMVDDPELIDLVEVEVQELLGK
                     YKFNDSPVIRGSALMAMEATGDLSRNSTNEYVKAIWQLADTLDAYIPDPERPIDKPFL
                     MPIEDVFTITGRGTVVTGRIERGQIKVGEEVEIVGMKDERRKTVCTGVEMFRKLLDYG
                     QAGDNVGLLLRGIDYKDVERGQVVCKVASINPHKKFSSEVYILSKEEGGRHKAFFTGY
                     RPQFYFRTTDVTGGIKLPQGVEMAMPGDNVKMEAELIVPVAMELGLRFAIREGGRTIG
                     AGVVTEILE"
     gene            2698..3006
                     /gene="rpsJ"
                     /locus_tag="PK717_01565"
     CDS             2698..3006
                     /gene="rpsJ"
                     /locus_tag="PK717_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR46388.1"
                     /translation="MSTSKMRIKLRAFDHVILDQSTAKIVETARRAGARIAGPVPLPT
                     SRQVFTVLRSPNIDKDSREQFEMRVHKRMLDIFEPSQEITKALMNIDLPAGVDIEIKL
                     "
     gene            3019..3630
                     /gene="rplC"
                     /locus_tag="PK717_01570"
     CDS             3019..3630
                     /gene="rplC"
                     /locus_tag="PK717_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR46389.1"
                     /translation="MAKGLVGKKIGCTQLWEEDSCSMVTVIQVEPNVVLEQKTTATHG
                     YNAVKIGFGDTKPSKVPRPVSGVFSKLGLPLHRYMMEFREVSADVGSKLGVDQFQPGD
                     KVNVIGRSRGMGFQGVMKRHGFAGGDDSHGAMEFHRRPGSIGCRLTPGHTIKGMKMAG
                     HMGSERVTVKGLKVVKIDAERGLLLLKGAIPGAKGGRVVVMGK"
     gene            3638..4258
                     /gene="rplD"
                     /locus_tag="PK717_01575"
     CDS             3638..4258
                     /gene="rplD"
                     /locus_tag="PK717_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR46390.1"
                     /translation="MNIPVYNTNGDKVKDYNAPDHIFNVERHEVAEYYSILQRLAGLR
                     RGTASTKIKSEVRGGGKKPFRQKGTGRARQGQSTSPINPGGGIVFGPKPRSYSFPLNR
                     KFRRLALFSALSSKVGSGDMRVIEGLKIEEPKTKKALDLISKNMVHKTLVIYDPSENG
                     EFGLALANTIDAIAIPYNFLNVYDILNSQRVLVTAEAADGIKEVWS"
     gene            4255..4548
                     /gene="rplW"
                     /locus_tag="PK717_01580"
     CDS             4255..4548
                     /gene="rplW"
                     /locus_tag="PK717_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR46391.1"
                     /translation="MSEKRKICLIRPIISEKNTDLEAMHKYVFQVIEGANSRWVKEDV
                     EQVFKVKVDDVNLLHIPGKRKRVRMFFRKVGGMKKAIVTLKKGQKIDILEQQR"
     gene            4556..5380
                     /gene="rplB"
                     /locus_tag="PK717_01585"
     CDS             4556..5380
                     /gene="rplB"
                     /locus_tag="PK717_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR46392.1"
                     /translation="MPVKKFSPTSAGIRHKVAVVYDEITTNKPEKSLTKGFSKSAGRS
                     NGKIAIRHHGGGHKQRYRIIDFKRDKALPGKISSVEYDPNRSAFIALVIYGDGEKRYI
                     LAPDGIKVGDMIISSDNADIKPGNTLPLSKMPLGTSIHNIELIPGRGGVLVRSAGTSA
                     QLLAKETDFCHVRLPSNEVRLIASNCRATIGTVSNVDHEKEKLGKAGVRRWRGWRPTV
                     RGYVMNPIDHPHGGGSGKCPTGHPGPLTPWGKPTRGYKTRNPKRSSSKYIVRRRKA"
     gene            5392..5676
                     /gene="rpsS"
                     /locus_tag="PK717_01590"
     CDS             5392..5676
                     /gene="rpsS"
                     /locus_tag="PK717_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR46393.1"
                     /translation="MSRSSSKGPFVDEKLLKKVQEMNASGQKRIIKVWCRRSVITEDM
                     IGFTIAVHNGKNHIPVYVTKPMVGHKLGEYAPTRVFHGHRRPTERSSALT"
     gene            5687..6028
                     /gene="rplV"
                     /locus_tag="PK717_01595"
     CDS             5687..6028
                     /gene="rplV"
                     /locus_tag="PK717_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR46394.1"
                     /translation="MKVHAIHKHAQVSPQKARKVICLIRGKDVQEAEYILRSLPHKSA
                     RLILKVLLSAKANAIHNFELNGDSLKVSEAIINMGTPWRRIMPKGMGRADIIKKRTSH
                     ILVAVSPKEDK"
     gene            6028..6684
                     /gene="rpsC"
                     /locus_tag="PK717_01600"
     CDS             6028..6684
                     /gene="rpsC"
                     /locus_tag="PK717_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR46395.1"
                     /translation="MGQKCHPYGFRLGVIQPWRSNWFADKEDFKKYLSEDLAIRESIE
                     KLGLEAGVADVIIERRSNEVRVIIRTAKPGVVIGRKGASADKLKKQLQGLITAKNLKL
                     DIVEMRSPDLSSKLVAEWIADQINRRIPYKRAMKQAMARSMKSGAKGIKIMASGRLAG
                     AEIAREEWFREGRVPLHTLKADIDFNITHVVTKFGCIGLKVWIYKGDKPTSGPYSVKR
                     "
     gene            6693..7121
                     /gene="rplP"
                     /locus_tag="PK717_01605"
     CDS             6693..7121
                     /gene="rplP"
                     /locus_tag="PK717_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOR46396.1"
                     /translation="MLQPERTKYRKQQRGTMHGLSKGGNYLNFGDFGIQALDRDWITA
                     RQIEAVRLAVTSRIPKDAKMWMRIFPDKPISKKPLETRMGKGKGDTEQWVAVVRPGRI
                     IVEFSGCDEKTAKYIAHIVSAKLPIKSRFVSRKLESEAAL"
     gene            7118..7309
                     /gene="rpmC"
                     /locus_tag="PK717_01610"
     CDS             7118..7309
                     /gene="rpmC"
                     /locus_tag="PK717_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR46397.1"
                     /translation="MKLYKIKEMSTVEMEKKVEELEKELALLVFRHKNRQLDKVMKLR
                     EVKRDIARLKTFIHQAKEE"
     gene            7314..7583
                     /gene="rpsQ"
                     /locus_tag="PK717_01615"
     CDS             7314..7583
                     /gene="rpsQ"
                     /locus_tag="PK717_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOR46398.1"
                     /translation="MADKLITRTGVVVSDSMNKTIGVVVERMVTHALYKKRVKKSRKY
                     LVHDEHEQAKIGDVVEFIDCQPISKKVHWRLLSVVEQGKQSEVQK"
     gene            7580..7951
                     /gene="rplN"
                     /locus_tag="PK717_01620"
     CDS             7580..7951
                     /gene="rplN"
                     /locus_tag="PK717_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR46399.1"
                     /translation="MIGIYSRLNVADNSGAKVVSVIRVIGTGNKIFGFVGDRIKVTVK
                     QAQPNSAIPKGSVQTAVIVRTSHAVVRKDGSRVRFDDNAAVIIDPGDGNPKGTRIFGP
                     VCRELRERGYMKIISLAPEVV"
     gene            7951..8292
                     /gene="rplX"
                     /locus_tag="PK717_01625"
     CDS             7951..8292
                     /gene="rplX"
                     /locus_tag="PK717_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR46400.1"
                     /translation="MKTPERFTMRVKESDMVEVISGKSKGHRGRIKKVLPFTNRVIVE
                     GANMIKKITKPTQRNPKRDIISLEAPLHRSNVMLVCPNCKKTTRIAAKIMPNGKKIRA
                     CNKCGELLDKA"
     gene            8302..8955
                     /gene="rplE"
                     /locus_tag="PK717_01630"
     CDS             8302..8955
                     /gene="rplE"
                     /locus_tag="PK717_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR46401.1"
                     /translation="MAEKKPQAQSKKGDSKSGPSLIGKSGVSDKVQTKDLKIQPYQTK
                     YKSEIIDQLRKQFNYENIMQVPKIVKISINRGVGEATANPKALDSTVEEIYLISQQRP
                     TIKKAKKSIANFKLRAGVPVGCMVTLRGSRMWAFVGKLIDQALPRIRDFKGLSPHSFD
                     GRGNYTFGIKEQLIFPEIDYDRVDKTRGFNVSIVTSAHTDEEARALLKAIGFPFREN"
     gene            8966..9151
                     /locus_tag="PK717_01635"
     CDS             8966..9151
                     /locus_tag="PK717_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOR46402.1"
                     /translation="MARLALINKSNKKPKFSTRKRNRCMICGRPRAYYGQFGLCRHCL
                     RKLANEGKIPGLVKASW"
     gene            9161..>9349
                     /gene="rpsH"
                     /locus_tag="PK717_01640"
     CDS             9161..>9349
                     /gene="rpsH"
                     /locus_tag="PK717_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR46403.1"
                     /translation="MYTIDPIADMLTRIRNANLARAQVTSVPHSSIKAEIARILYETG
                     FISEFKTQEIEGKKFIRVY"
BASE COUNT         2731 a         2194 c         2298 g         2126 t
ORIGIN      
        1 tttatctcat gctcgaagtt cccttgtaaa tcgtattgcc tggcataatt ttttgggcca
       61 tttttgaatg tggccaaaat cttgccgtat gaaagtactg cattttccat gcatgcgcca
      121 ggatcatgga ttattgtttg ccagttttct tttttggggt ttgcagggtc tatcatcacc
      181 actttgccca tcgggcagtc gatattggtg gaaaaaatta attgtttacc tattactccc
      241 atgtatgaaa attggtctgt attattttca ataattggga tcttgttttt agggtagtca
      301 atctcttgca ggtagtagtc atttatttcc ccgtattcat tgatcagaag atatttttca
      361 tcaataatgt cactgcaagc cagcattacc aagtctatct gggatttaag taccagaaca
      421 tcctgctcct ggcctgttcc aactttgtga tagtacaatt tgggcctggt caatttctgt
      481 gtgagcctct tctcttcaat cacttcatcg aaccttgtgt agtagaatcc gctaccatca
      541 agccacgttg tgccggcgta tctgactttt gtgagtcttt ctggtaaaaa ttcctttttc
      601 tcgaggtcgg ccaccataat gtctgtccaa tcggagccat cctctttgaa tgatattgca
      661 gcctttgatc cgtctggcga aaatgatgag atgcctacat attgcctgtc tggatcaatg
      721 tcatatttga ttgcctcata aggtttcctt tttataaggt ccatggccat aattttttgt
      781 gtgccggaaa aagtgaaata gagttttcca tttttcacga tcgaggtgtg gccaaaagaa
      841 tgcaaaaatg gagggaaagg caatttgtag gctgtgagtt cagttattct ttgccttatt
      901 ttatccctga atggcatttt ttcaatttcg ttgctgaaaa attctcgttg tgcttcgacc
      961 cagttttttg tttcctcgga attttcatcc tccagccacc tgtacggatc agctactttt
     1021 acaccaaaat aatcatccac tacattgccc ttctctgcct ttgggtaatt tttgcccatc
     1081 tcccgctcct ttttctgagt tttgaattgt cactacatta ttaaatcaat attgttaagt
     1141 ggcaacttat atacaaactg ggctttttgt gtataataat tgtgcgcctg aaattccaag
     1201 gagaattggg atttaacttg ccttatcatt gacattggca ttgtgggcaa taaagtatta
     1261 ctctggcatt gcggggtaga gcagcctggt agctcgtcgg gcccataacc cggaggtcgt
     1321 tggttcaaat ccaacccccg caaccaaatg tatgcccacg tagctcagat ggcagagcat
     1381 gtccttggta aggacaaggt cagcagttcg attctgctcg tgggctccat aaagatgcct
     1441 cacgagggcc ttttttaaaa gcattaagga gagtaagaat atggcaaaac aaaaatacga
     1501 gcgcacaaaa ccgcacgtca acgtcggaac gatcggccac atcgatcatg gcaagacgac
     1561 actaacgtct gccatcacga agaccctcgc caccctcggc cacaccgtag caaaagccta
     1621 cgatgaaatt gacaacgcgc cagaggaaaa ggcaaggggc gtcaccatca acatccacca
     1681 cacagagtat gagactgaga agaggcacta tgcccacatc gactgcccag gtcatgctga
     1741 ctacatcaag aacatgatca ccggtgctgc ccagatggac ggagccattc tcgtcgtcgc
     1801 cgcctctgaa ggtccgatgc cacagaccag ggaacacatc ctcctcgccc gtcaggtcaa
     1861 cgttccaagg atcattgtct tcctcaacaa gatagacatg gttgacgacc cggaactcat
     1921 cgacctggtt gaggttgaag ttcaggaatt gcttggaaag tacaaattca atgactcacc
     1981 agtcatcagg ggctcagccc tcatggcaat ggaagccaca ggtgatctct caagaaacag
     2041 cacaaacgaa tacgtcaagg ctatctggca gcttgctgat acccttgatg catacattcc
     2101 tgacccggaa cgtccaatcg acaagccatt ccttatgcca atcgaagacg tcttcacaat
     2161 cactggccgt ggcactgttg tcaccggtcg tattgaaaga ggacagataa aggtcggcga
     2221 ggaagtcgaa atcgttggaa tgaaggacga gagaaggaaa accgtctgca ccggtgtcga
     2281 aatgttccgc aagctccttg actacggcca ggctggcgac aacgtcggac tcctcctcag
     2341 gggcatcgac tacaaggatg tcgaaagggg acaggtcgtc tgcaaggttg catcaatcaa
     2401 cccgcacaag aagttctcct ccgaagtcta catcctctcc aaggaagagg gtggacgtca
     2461 caaggcattc ttcaccggct atcgtccaca gttctacttc aggaccactg acgtcaccgg
     2521 cggcatcaag ctcccacagg gtgttgaaat ggccatgcca ggtgacaacg tgaagatgga
     2581 agccgaactc atcgtcccgg tagccatgga actcggactc cgcttcgcca tccgtgaagg
     2641 tggccgtacc atcggagccg gtgtagtcac cgaaatattg gagtaactgg agcgaaaatg
     2701 tcgacatcca aaatgagaat taaactaagg gcttttgacc acgtcattct tgaccagtca
     2761 acagccaaga tcgtggagac ggccagaagg gctggcgcca gaatcgccgg tccggttccg
     2821 ctcccaactt ccaggcaggt ttttactgtt cttcgttcac ctaacattga caaggacagc
     2881 cgcgagcagt tcgagatgag ggtccacaag agaatgctgg acatcttcga gccatcccag
     2941 gaaataacaa aggctctcat gaacatcgat ttgcctgccg gagtggacat cgagataaaa
     3001 ctctaaggaa ggtgctacat ggccaaggga ttggttggca aaaaaatagg ttgcacacag
     3061 ctttgggaag aagacagctg ttcaatggtg acagtgatcc aggtcgagcc caatgttgtg
     3121 ctagagcaga aaacaactgc aactcatggc tacaatgccg tgaaaattgg cttcggtgac
     3181 acaaagccca gcaaggttcc acgtccagtt tctggcgtat tctccaagct tggtcttccg
     3241 cttcaccgct acatgatgga atttcgtgaa gtctctgctg atgttggctc caaacttgga
     3301 gttgaccagt tccagccagg cgacaaggtc aatgtcattg gccgttcgcg tggaatgggc
     3361 ttccagggtg tcatgaagcg ccatggattt gccggtggag atgattccca cggtgcaatg
     3421 gagttccata gacgtccagg ttccattggt tgccgtctca caccagggca taccataaag
     3481 ggtatgaaga tggctggcca tatgggtagt gagcgtgtca cagtcaaggg cctcaaagtt
     3541 gtaaaaattg atgctgaacg tggccttctg cttctcaagg gtgcaatacc aggcgccaag
     3601 ggtggcagag ttgtggtcat ggggaagtag gtcaaacatg aatatcccag tctacaacac
     3661 gaatggtgac aaagtaaaag actataatgc gccagaccac atctttaatg tggaacgcca
     3721 tgaagttgct gaatactaca gcatccttca gagacttgca ggtcttcgca gaggcacagc
     3781 ctcaacaaag ataaaatctg aagtcagggg cggaggaaag aagcccttca gacaaaaagg
     3841 cacaggaaga gcacgtcagg gacagagcac atcaccgatc aatccaggtg gtggaatagt
     3901 gttcggacca aagccgcgtt cttacagctt ccctctgaac cgcaagttta gaagactagc
     3961 ccttttctcg gcgctttcat caaaagtcgg atcgggcgac atgagagtca ttgaaggcct
     4021 caagattgaa gagccaaaaa caaagaaggc gcttgatctt atctccaaga atatggttca
     4081 caagaccctt gtgatctacg atccttcaga aaatggcgag tttgggcttg cacttgcaaa
     4141 cacaatagat gctattgcaa tcccttacaa tttccttaac gtctacgaca ttttaaacag
     4201 ccagcgtgtt cttgttacag ccgaggccgc tgacggaata aaggaggtct ggtcatgagc
     4261 gaaaagagaa aaatctgcct tatcagaccg ataatatctg aaaagaacac agaccttgaa
     4321 gcaatgcaca agtatgtttt tcaggtaata gaaggtgcaa actctcgctg ggtcaaggaa
     4381 gatgtggagc aagtgttcaa ggtgaaagtt gatgatgtaa acctgctaca cattccgggc
     4441 aagaggaaac gtgtcaggat gttcttcagg aaagttggtg gcatgaagaa ggccattgtt
     4501 accctgaaga agggccaaaa gatcgacatc ctcgagcagc aaaggtaggt aaacgatgcc
     4561 agttaaaaag ttttcgccaa cttcagcagg cattcgccac aaagtggcgg ttgtctatga
     4621 cgaaataaca acaaacaagc cagagaagtc cctgacaaag ggtttctcga aatcggccgg
     4681 caggtcgaat ggcaagatcg ccatccgtca tcatggtggt ggccacaagc aaagatatcg
     4741 tatcattgac ttcaagagag acaaagctct tcctggcaag ataagttctg tagagtatga
     4801 tccaaatcgc tccgctttca tagcactcgt tatctatgga gacggtgaga aacgttacat
     4861 tcttgccccg gacggcatca aagttggtga catgatcatc tcttctgaca atgccgacat
     4921 aaagcctggc aatacattgc cgctttcaaa gatgcctctt ggaacaagca tccacaatat
     4981 cgagctgatc ccgggaaggg gcggagttct tgttcgctca gcgggcacct cggcccagtt
     5041 gcttgcaaaa gaaacggatt tctgtcatgt caggctgccc tccaacgaag taagacttat
     5101 tgcttcaaac tgcagggcaa ccatcggaac agtatcaaac gttgaccacg agaaggaaaa
     5161 actcggaaag gccggcgtta gaaggtggag gggatggcgc ccaacagtca gaggctacgt
     5221 catgaaccca attgaccacc cgcacggtgg aggatcgggc aaatgcccaa cgggacaccc
     5281 aggaccgctt accccatggg gcaaaccaac cagaggttac aaaacccgca acccgaagag
     5341 atcttcttca aagtatattg tgcgcagaag gaaagcatag gagtagagca catgagcaga
     5401 tcatccagca aaggaccatt tgttgacgag aaactcctca agaaggtaca ggaaatgaat
     5461 gcatccggcc agaagagaat catcaaggta tggtgcaggc gttccgttat cactgaggac
     5521 atgattggct tcaccattgc tgtacacaac ggaaaaaacc acatcccggt gtacgtgacc
     5581 aagccaatgg ttggccataa acttggcgaa tacgctccaa cccgagtatt ccacggccac
     5641 aggcgcccga ccgagcgctc cagcgcctta acataagagg tgtgaaatga aggtccacgc
     5701 aatacataaa cacgcgcagg tatcgcccca gaaggccaga aaggtcatct gcctcattag
     5761 gggcaaggac gtccaggaag ccgagtacat cctcaggtca ctcccgcaca agtctgccag
     5821 attgatactg aaggtcctcc tttccgcaaa ggcaaatgcc atccataatt ttgaactcaa
     5881 tggcgacagc ctcaaggtgt ccgaagcgat catcaacatg ggaactccat ggagaaggat
     5941 catgcccaaa ggcatgggca gggctgacat cattaagaaa cgcaccagcc atatcttggt
     6001 tgctgtatca cccaaggagg acaaataatg ggccaaaaat gtcatccata tggttttagg
     6061 cttggagtca tccaaccctg gagatcaaac tggtttgcag acaaggaaga tttcaagaaa
     6121 tacctctccg aagacttggc cattcgtgag tcaatcgaaa aactcggtct tgaagccggt
     6181 gttgccgatg tgatcatcga acgcagatca aatgaagtca gggtgattat tcgtacagcg
     6241 aagccaggag ttgtaattgg aagaaagggt gcctcagctg acaagcttaa aaagcagctc
     6301 cagggactca tcaccgccaa gaacctcaaa ctcgacatcg tcgaaatgcg ctcaccagac
     6361 ctttccagca aactcgttgc tgagtggatc gcagaccaaa tcaaccgcag gatcccgtac
     6421 aagagagcaa tgaaacaggc aatggcaaga tcaatgaagt ctggagcaaa aggcataaag
     6481 atcatggcat cgggcagact tgcaggtgct gaaatcgccc gcgaggaatg gttcagggaa
     6541 ggaagggtcc ccctccacac cctgaaagcc gatattgatt tcaacattac tcacgttgtg
     6601 accaagttcg gttgcatcgg cttgaaagtt tggatctaca aaggcgacaa gccaaccagt
     6661 ggtccatact ccgtcaagcg ctaggaggag aaatgctaca accggaacgc acaaaatata
     6721 gaaaacagca gcgcggaacg atgcatggcc tctcgaaggg cggaaattat ctgaacttcg
     6781 gtgactttgg catccaggcg ctggaccgtg attggataac agcccgccag attgaagctg
     6841 tcaggcttgc agtcacatcg cgtatcccaa aggacgcaaa gatgtggatg aggattttcc
     6901 cggacaaacc gatttccaaa aagccacttg aaacccgtat gggaaaggga aagggagaca
     6961 ctgaacagtg ggtcgccgtt gtccgccccg gaagaattat tgttgaattt tccggatgtg
     7021 atgagaagac agcaaagtat attgcccaca ttgtgtctgc aaagctcccg atcaaatcca
     7081 gatttgtgtc acgtaagctg gagtcggagg cggcactatg aagctctata agataaaaga
     7141 gatgtcaacc gtcgaaatgg agaagaaggt tgaggaactt gaaaaagaac tcgctcttct
     7201 ggtttttcgc cataagaaca ggcaattgga caaggtcatg aagctcagag aagtcaagcg
     7261 cgacatcgcc aggctgaaga cctttatcca tcaggccaag gaggagtaat aacatggccg
     7321 acaaacttat tacaagaaca ggcgttgttg tatcagactc gatgaataaa accatcggcg
     7381 ttgttgtaga gcgcatggtc acacacgccc tttacaaaaa gagagtaaaa aaatccagga
     7441 aatatctggt ccatgatgag cacgagcagg caaaaattgg tgatgtggtt gagtttattg
     7501 attgccagcc aatctcaaaa aaggtccact ggcgcttact tagtgttgtt gagcaaggaa
     7561 aacagtcgga ggtgcagaaa tgataggcat ctactcaaga ctgaatgttg ctgataacag
     7621 cggtgcaaag gttgtttccg taatccgtgt cattggcact ggcaacaaaa tcttcggatt
     7681 tgttggcgac agaatcaaag tcacggtcaa acaagctcag ccaaactctg ccatcccaaa
     7741 aggatcggtc caaaccgcag ttatcgttcg cacctcacat gcagttgtcc gcaaagatgg
     7801 ctctagggtg cgttttgatg acaatgctgc ggtaataatt gatccaggtg atggaaatcc
     7861 aaaaggtacc cgtatcttcg gacccgtctg tagggaactg cgtgagaggg gttacatgaa
     7921 gatcatttct ctcgctccgg aggtggtgtg atgaaaaccc cagagagatt tacaatgaga
     7981 gttaaagaat cggatatggt agaagtcatc tctggcaagt ccaaagggca taggggaaga
     8041 atcaagaagg ttctcccgtt cacaaatcgc gtcatcgttg aaggcgcgaa catgataaag
     8101 aaaatcacca agccgacaca gagaaatcca aagcgtgaca ttatatcgtt ggaagcccca
     8161 ctacatcgct caaacgtgat gctagtgtgc cccaactgca aaaaaaccac caggatagca
     8221 gccaagatta tgccaaacgg aaagaaaatc cgtgcgtgca ataaatgcgg cgagctcctg
     8281 gacaaggcat gaggttagaa aatggctgaa aagaaaccac aagcacaatc aaaaaaggga
     8341 gattctaaga gcggtccatc cctaattggt aagagtggtg tatccgacaa agtacaaacg
     8401 aaagatctga agatacaacc ttaccagacc aagtacaaaa gcgagatcat agaccagctg
     8461 agaaagcagt ttaattatga gaacatcatg caggtcccaa agattgtaaa gatctcgatc
     8521 aatcgcggtg ttggcgaagc aacggcaaat ccgaaggctc tcgattcaac agtcgaggag
     8581 atctatctca tctcccaaca acgccccaca atcaagaaag ccaaaaagtc aatcgcaaac
     8641 ttcaaactca gagctggagt tccggtcggc tgtatggtaa cactcagggg aagcaggatg
     8701 tgggcttttg ttggaaagct catagaccag gccctcccaa gaatcaggga cttcaagggt
     8761 ctctcgccac acagctttga tggcaggggc aattatacct tcggtatcaa agaacagctc
     8821 atattccccg agatagacta tgacagagtt gataaaacaa ggggatttaa tgtctcgatt
     8881 gtcacttccg cccacaccga tgaggaagca agggcgcttc taaaggcaat cgggttcccg
     8941 ttcagggaga actaggagaa ggcttatggc aagactggcg cttattaaca aatcaaacaa
     9001 gaagccgaaa ttctcgaccc gcaaaaggaa ccgttgcatg atatgcggaa ggccgagggc
     9061 ctattacggg cagtttggtc tttgcaggca ttgcctccgc aaacttgcca acgagggcaa
     9121 aattccgggc cttgtaaagg cttcttggta ggggtgtggc atgtatacaa tagacccaat
     9181 cgctgatatg ctcacaagaa tccgcaatgc aaacctggca agagcgcagg taacttcggt
     9241 cccgcattct agcatcaagg ccgagattgc aaggatactt tatgagacag gcttcatctc
     9301 cgagttcaaa acccaggaga tcgagggcaa aaagtttata agagtatat
//
LOCUS       DAOSRI010000030         9304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7122_length_9304_cov_4.284679, whole genome shotgun sequence.
ACCESSION   DAOSRI010000030 DAOSRI010000000
VERSION     DAOSRI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_9304_cov_4.284679"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..875)
                     /locus_tag="PK717_01645"
     CDS             complement(78..875)
                     /locus_tag="PK717_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46404.1"
                     /translation="MRLSEVLSKSPECDYSQVDGFLINKKGNTGQQITLSVGEVMLNY
                     FCVQCEDFRTFTSKGKLCCVFVNKHIISIDCILACGCGSNVQAWFLVECEDDICGQAP
                     KVRIIKHSEKLSAMVKTKNVQYGEFSSLLDMAECAYREGLGAGAIVYLRKTFEKITAQ
                     TADVMGISYDKHEGGNPKNFRVLLEEVDKKCAIIPKEFSNNGYKLFRELSSIVHGEYN
                     EEQGLKKFEPLRRLVIGTLENVRINRELRNAIKSLGWKEPLGGVGDE"
     gene            complement(891..1385)
                     /gene="rhuM"
                     /locus_tag="PK717_01650"
                     /pseudo
     CDS             complement(891..1385)
                     /gene="rhuM"
                     /locus_tag="PK717_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(1382..2950)
                     /locus_tag="PK717_01655"
     CDS             complement(1382..2950)
                     /locus_tag="PK717_01655"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOR46405.1"
                     /translation="MTNNTKEQERAELHRMIWSIANDLRGSVDGWDFKQYVLGMLFYR
                     YISENLAGYINIGEWEAGHKDFDYAKLPDQEAEQAREDLLNTKGFFILPSELFENVRR
                     RAPGDENLNETLEKVFKNIEASALGTNSEDDFKGLFDDIDVNSNKLGSTVAKRNERLV
                     KLMNGVGEMKLGNYKDNTIDAFGDAYEFLMGMYASNAGKSGGEFFTPQEVSELLTRLA
                     LVGKTEVNKVYDPACGSGSLLLKFAKILGKENVRLGFFGQEINITTYNLCRINMFLHD
                     IDYDKFDIGHGDTLTDPLHWDDEPFEAIVSNPPYSIKWPGDSDPILINDQRFSPAGVL
                     APKSKADLAFIMHSLSWLATSGTAAIVCFPGVMYRGGAEQKIRQYLIDNNYIDCVIQL
                     PDNLFYGTSIATCIMVLKKSKAENSTLFIDASKEFVKVTNNNKLKQENIDAILGAFTS
                     RKSMAHFSRLVPNADIAAQDYNLSVSTYVEQEDTREKIDIVTLNAEIEKIVAREQVLR
                     DEIAKIIAEIEVGT"
     gene            complement(2943..3174)
                     /locus_tag="PK717_01660"
                     /pseudo
     CDS             complement(2943..3174)
                     /locus_tag="PK717_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870276.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3430..3909
                     /locus_tag="PK717_01665"
     CDS             3430..3909
                     /locus_tag="PK717_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46406.1"
                     /translation="MKKTLLVLVFLVSIIFVVSCGQATPDQTQTNQETQKNQYAKTKP
                     VALLSIEASDEERGLFNMQVQNMSDKMIASFSWTVVLYDALGKVLGTQDSFFEDSQYG
                     LSPSQAVQGSFVSNYKDAVKAKVVFKDAVLFVDFKVGTTKNWVNEDYNKELQELLKG"
     gene            complement(3912..6884)
                     /locus_tag="PK717_01670"
     CDS             complement(3912..6884)
                     /locus_tag="PK717_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOR46407.1"
                     /translation="MRRPTGQGYRKRQNGSCRKQGWCNLVPISLEITDFLSYRGFHQL
                     DFSSFHVASITGMNGAGKSAILDAITWALFGQARKPTLIRSKTKSDRETVINDDANTC
                     EVSLTFRIGQNTYLVDRAMDRGGAIRLQFRQITRDGENDLRGKSTPETDQAIETELGF
                     TYEVFASSSFITQGDSARFMEAKPKERREILAQILQLDKYGACKEIADSRLKSLKEET
                     SAQKALLEDKSKTSERIPQIESDLAQAEYDLNSAERQWEECRKNLELLRKNLSVAEAK
                     RKTLEETKTKTTKLEKELGEIASSIAELDKRISQYKKLLENETQVNLGFEKLGQKRRE
                     LERLTEKEARVSTLERAKTQAQTKIDIKAKELEIRLVQLSTQIDESLKAQTMLEGLQK
                     EVDRLTEEKAKVAKIREEKHSWDLSFQQAKNTLAGAEKKFAEQQGRLEKALKLTGVES
                     ASGIDNTLGKVRELQAGIPTLEEEIASIKKSVSDTESEKATAIAEIDHLEKEIQVLGE
                     GETGKCPLCGQELPSGGSAKLVEEKREKIDKCKTSLQGINEKIGPMKQSLANAEKNLD
                     LAKQSVGKIPLLEQAISISSESGQISQELEEKRKSFEEARKGFLLFEETNGLVLQQEN
                     QTLAQYEQASKQLTLTAEKASKIENLKEHEASLQKEFISKSFATEELELIEKTKKEIE
                     AEGFDQMELSQVKKAVSELESFAQQKTELEVAKASLAGATEQSETAKKRFSSINGDIV
                     ESQKLLLELGDTEREYQEASQKTGAEEQKEKLLQKQRDQAFSSRDVLAKSLGDAKKAL
                     EESSQIQEKLVTLEKDQRIFERIKEMFGIDGIPSRVLQGVAPQLEEIANSVLQRISQG
                     HTGSESLRMEIKLERESKDKSLSTLDVILTDGQTARPYELFSGGERFRADFAIRLALS
                     KLLAQRAGAKLQTLVVDEGFGTQDSEGIKRLVEAINEMSSDFEKILVVSHVEEMRNSF
                     ERKILVTRDDDGSHFEVV"
     gene            complement(6814..8316)
                     /gene="sbcD"
                     /locus_tag="PK717_01675"
     CDS             complement(6814..8316)
                     /gene="sbcD"
                     /locus_tag="PK717_01675"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOR46408.1"
                     /translation="MSRIKVLHTADLHLGVETYGVTNQDGINSRIKDYLETLDWLVLQ
                     AKSLDCDAFLVAGDIFDNERPTNYVISEFAKRIRNLVDSSITVILTPGNHETSSSSRV
                     PSALEVFRALHPQKDGTEIACHVLGCQPSKPGDTETMGGISTIPTKSGNLQILAMPYP
                     RRSEILTSEQMKNRGREEVKKLAATIFLDRIQELARKALPGLPTIFVGHFGIKEAELR
                     PGVKGYLADDVIISTYDLMSKLSDSQAKFGYVALGHYHSPQMPSPNLVAPSLDEDMDG
                     ETSILCGTNPCRDWELSSNETVAPDPDSLFRDGKPVRKIQVAYSGAPSRRDFPDGART
                     RMFMELDLTPEGSSGKCRKVEHSRSLRQLTVDNALDWKEELEAKFLKSEFWRQPYEYA
                     ATGCGQEQIEKTMKGPLPIFRLRLPEEARSIWPAIKSWLDTLPMFDKIIAPYYKPSAR
                     ESQKAVIAQTESPVEAVKSYLDSLQDDFAKTNRARIQEEAERIMQEARVV"
     gene            complement(8303..9001)
                     /gene="rsmI"
                     /locus_tag="PK717_01680"
     CDS             complement(8303..9001)
                     /gene="rsmI"
                     /locus_tag="PK717_01680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOR46409.1"
                     /translation="MTGTIFVCATPIGNLDDASPRLIQTLSQCPVIMAEDTRRTLKLL
                     THFGIKAQLFPLHIHNESISSNKALQILMMGQNIAIVSDAGVPTLADPGQILVDMAIN
                     HGIKVVPIPGPSAITTALSVSGFFAQTFTFRNFLPRKPGALGKALQQIAQEDRTTVVF
                     ESPFRMLALLESICQNIPDCQLLICREMTKIHEEYFRGTPKEALERQKNIQPKGEYTV
                     VIGFPNQQAEDEQD"
     gene            complement(8998..>9304)
                     /locus_tag="PK717_01685"
     CDS             complement(8998..>9304)
                     /locus_tag="PK717_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46410.1"
                     /translation="LKGVKDFLILDEQGKVLLRQTKGTGFSQGVLSGYLASVYSIADR
                     TTMPFTSNIAEISFDGEGGSVLVMKGKDKYFMLLLEGTPSMGIARLKLRTLVDNYEQ"
BASE COUNT         2056 a         2239 c         2052 g         2957 t
ORIGIN      
        1 tagtaatata ggcaatctca tcaagagctt tatactccac cccgtccgga caaaactcgt
       61 taatcaacct gtctagttca ctcatcaccg acaccaccta acggttcttt ccatcccaga
      121 gatttgattg cattccgtag ttctctgttg attctgacat tttcgagtgt cccaatcaca
      181 agcctacgca aaggctcaaa cttttttaat ccttgctctt cattatactc accgtgaaca
      241 atgctactta gctcacgaaa aagcttataa ccattatttg aaaattcttt tggtataata
      301 gcgcactttt tatcaacttc ttccaacaga acacgaaaat tctttgggtt tccaccttca
      361 tgtttgtcat atgaaattcc catgacatct gctgtttgtg cagtaatctt ttcaaatgtt
      421 ttccgtaaat atactatggc tccagcacct aaaccctctc gatacgcaca ttcggccata
      481 tctaaaagag atgaaaattc accgtattgt acattcttgg ttttcaccat agctgacagt
      541 ttttcactat gttttataat tctaactttg ggagcttgtc cgcatatatc gtcttcgcat
      601 tcaaccaaaa accatgcttg tacattcgac ccacatccac aagccagaat gcagtctatg
      661 ctaataatat gtttgtttac aaaaacgcag cataatttgc ctttcgatgt aaaagttcga
      721 aaatcttcgc attgaacaca gaaataattt aacataactt ctccgactga taacgttatt
      781 tgctgacccg tattgccttt tttgttaatc aaaaatccat ctacttgcga atagtcgcat
      841 tctggagatt tagagagtac ctcacttaaa cgcataggtt acgttctctc tatttctgtg
      901 atttgctgcc agatgcggcg ttcgctggcc cgaatggaac gaacgcgctc cagcagttct
      961 ctgaaatagt ctttttcaaa ggctgtcttg ccctgtttca gccgttcatc gtcaagggta
     1021 aaacctttta ttatgtactc tttcaatacc ccagtcgccc agatgcgaaa ttgggtggcc
     1081 cgctttgagt ttacacggta accgacagaa atgatggcat caagattgta atagtcaacg
     1141 tttcgcctga ccgctctttc gccctcggtt tgaactgttt ccattttgga aacagttgca
     1201 tcttttctta gttcgccctc atcgtaaata ttttgtaaat gctttgatac tgcagggata
     1261 tttataccaa ataattcggc catagccttt tgcgtcaacc aaatcgtttc atctttgatg
     1321 accgcattga ctgtcacatt ttctgaaacg gattgataaa tcagaaactt aaacccgtct
     1381 ttcatgtgcc cacctctatt tccgcgatga ttttggcaat ctcatcacgt aaaacctgct
     1441 cccgcgctac aattttctct atttcagcat tgagggtgac aatatcaatc ttctctctgg
     1501 tgtcctcctg ctccacatag gtggagaccg aaaggttgta gtcctgggcg gcaatatcag
     1561 cgtttggcac aagccttgaa aaatgcgcca tgctttttct gcttgtaaaa gcgcccagaa
     1621 tggcatctat attttcctgt ttcaacttgt tgttgttcgt aaccttgacg aactcctttg
     1681 acgcgtcgat gaaaagggtg ctgttttctg ccttggattt tttgagcacc atgatgcagg
     1741 tggcaatgct cgtgccgtaa aacaggttgt ccggcagctg gatcacgcaa tcgatgtagt
     1801 tgttgtcaat cagatattgc cgaatttttt gttccgcacc accgcgatac atcacaccgg
     1861 gaaagcatac aatcgccgcc gttccgctgg ttgccagcca ggaaagactg tgcatgataa
     1921 aagcaagatc agccttggat tttggagcga gcacgccagc tggtgaaaag cgctggtcgt
     1981 tgatcaaaat cggatcgctg tcgccgggcc acttgatgga ataaggtgga ttggaaacta
     2041 tagcttcaaa cggctcatcg tcccaatgca gtgggtcggt cagcgtgtcg ccgtgaccga
     2101 tgtcaaattt gtcataatct atatcatgca ggaacatgtt gatacggcag aggttatagg
     2161 tggtgatatt gatttcctgg ccgaagaagc ccaggcgcac gttttctttt ccgagaattt
     2221 tggcaaactt tagaagcagg gagccagaac cacaggcagg gtcatagacc ttgttgacct
     2281 cggtttttcc caccagcgca aggcgggtga gcaattcgga aacctcctgc ggtgtaaaga
     2341 attcaccacc gctcttacct gcatttgaag catacatacc catcagaaat tcgtaggcat
     2401 ctccgaaagc gtcaatggtg ttgtccttgt agttgcccaa tttcatctcg ccaacgccgt
     2461 tcattaattt gacaagcctc tcgttgcgct ttgccactgt gctgcccagt ttgttgctgt
     2521 taacgtcgat atcatcaaac aaacccttaa agtcgtcctc gctattggtg cccagcgctg
     2581 aagcttcaat atttttgaac actttttcca gtgtttcgtt caggttctca tcacctggag
     2641 cgcgacggcg cacattctca aaaagctcgc tgggcagtat gaaaaacccc tttgtgttga
     2701 gaaggtcttc gcgtgcctgt tctgcttcct ggtcaggcag ttttgcataa tcaaaatcct
     2761 tgtgtcccgc ttcccattcg ccgatgttta tgtaacctgc aagattttcg gatatgtaac
     2821 gataaaacag cattccaagc acatattgct tgaaatccca gccatctaca ctcccgcgta
     2881 aatcattggc gatactccat atcatgcggt gcagttctgc acgttcctgt tcttttgtat
     2941 tgttagtcat ttttcagctc cccctcctca gctgtatctg gcactaattc aataatatct
     3001 tcgaaattga taccgagcgc tgagcagacc ttgtaagacc gtccatactg atttttcatt
     3061 tttaaaaagc tttggagttg atgatggact tgtaaacgta gctgcttgca aatcctcctt
     3121 gtttctgtgg aggtcaatta ataattccca aagcttttta tagctaacgg tcatttgttc
     3181 cgtcattttt gtcacctcgc caacaaaata taacttacca tagcaaaaaa acttcaagtt
     3241 tacaatgatt attgactata atcgaatatt ttcattgatt tgacgcctgt tctttatcat
     3301 ggctgtttag tttcaccggt tccctttctt ttatgctaga gtttcaaaag ataggagttt
     3361 gaatgagggc ttttatgtta ctgaaaacat aaaagttgac tttggtttgt ttggttttat
     3421 aaaaaggaca tgaaaaaaac tttgttggtc ctggttttcc ttgtttcaat catatttgtg
     3481 gtttcttgtg ggcaggcaac acccgatcaa acgcaaacta accaggagac ccaaaaaaac
     3541 cagtatgcaa aaaccaaacc tgttgcactc ctctcgattg aggcctccga cgaggagcgt
     3601 ggtcttttca acatgcaggt ccagaacatg tcagacaaga tgatagcctc gttctcatgg
     3661 acggttgttt tgtatgatgc tttgggaaag gtccttggca cccaggacag cttttttgaa
     3721 gactcgcaat atgggctcag tccatcccag gcggtccagg gttcctttgt ttcgaactac
     3781 aaagatgctg taaaagcaaa agttgttttc aaggatgctg ttttgtttgt tgatttcaaa
     3841 gtgggcacca caaaaaactg ggtgaatgaa gactataaca aagaacttca ggaattgcta
     3901 aaaggataaa actaaacaac ctcaaagtgt gatccgtcgt catcacgcgt cacgagtatt
     3961 ttcctctcaa agctgtttct catctcttca acatgtgaaa ctacaagtat tttctcaaaa
     4021 tctgaagaca tctcgtttat cgcctcgacc agcctcttta taccctcact gtcctgcgtt
     4081 ccaaacccct catccacaac gagcgtctgg agctttgcac cggccctctg tgcaagaagc
     4141 tttgaaagtg ccagcctgat tgcaaaatct gccctgaacc tttcccctcc ggaaaagagc
     4201 tcgtatggtc ttgctgtctg gccgtctgtg aggatcacgt caagcgtcga gagtgattta
     4261 tccttagatt ccctttccag ctttatctcc attcttagtg attctgatcc cgtgtgcccc
     4321 tgtgaaattc tctggagcac agagtttgcg atctcttcaa gctgtggagc aacaccctgg
     4381 agcacccttg aggggatgcc atcaattcca aacatttcct ttatccgttc aaaaattctc
     4441 tggtcttttt caagagtcac cagtttttcc tggatttgtg aggattcttc aagtgccttt
     4501 tttgcatccc ccagactttt ggcaagtacg tctctgctgg agaatgcctg atctctctgt
     4561 ttctggagca gtttttcctt ctgctcctcg gcacctgttt tctgactggc ctcctggtat
     4621 tccctttctg tgtccccaag ctcaagcagc agtttctggg attctacaat gtcgccattt
     4681 attgaagaga atcttttctt tgcggtctcg gattgctcgg tagcacccgc aagactggct
     4741 tttgccactt caagttccgt tttttgctgg gcgaaagact ctagctccga aactgccttt
     4801 tttacctgtg agagctccat ctggtcaaat ccttcggctt caatttcttt tttggttttt
     4861 tcaatcagtt caagttcttc tgttgcaaag gattttgaga tgaattcttt ctgtagcgaa
     4921 gcctcatgct ctttcaggtt ctcaattttt gaggccttct ctgccgtaag tgtcagctgt
     4981 tttgaggctt gctcatattg ggccaaagtc tggttttctt gttgaaggac caatccattt
     5041 gtttcttcaa acagcaaaaa ccctttcctg gcctcttcaa acgatttacg cttttcttca
     5101 agttcttgcg agatctgccc tgattctgac gagattgata tcgcctgttc aaggagaggt
     5161 atttttccaa ccgattgttt tgccaggtca agatttttct ctgcatttgc cagtgactgt
     5221 ttcattgggc cgattttttc gtttataccc tgaaggctgg ttttgcattt gtcgatcttt
     5281 tctctcttct cttcaacaag ttttgccgat ccgccagaag gcagttcctg accgcacaat
     5341 gggcattttc ctgtctcacc ctcgccaagg acctggattt ccttttcgag atgatcgatc
     5401 tctgcaattg ccgttgcttt ctcgctctcg gtatctgaaa cggatttttt tattgaggca
     5461 atttcttctt caagtgttgg gatgccagct tgcagttcac gtactttgcc aagcgtgtta
     5521 tcaattcctg aggcgctttc cacccctgtc agttttaggg ctttttccag cctcccttgt
     5581 tgctctgcaa attttttctc ggcaccggca agagtatttt ttgcttgctg gaaggagagg
     5641 tcccaggagt gtttttcttc tcttattttg gcgacttttg ccttttcttc cgtgagtctg
     5701 tcaacttctt tctgcagacc ctcgagcatt gtttgtgcct tcaatgactc gtcgatctgg
     5761 gtcgagagtt gaacaagtct tatttcaagt tcttttgcct tgatatcaat ttttgtttgt
     5821 gcctgtgttt ttgccctttc gagggtcgat acccttgctt ctttttctgt cagcctctcg
     5881 agttctcgcc ttttttggcc gagtttttcg aagccaagat ttacctgtgt ttcgttttcc
     5941 agcaattttt tgtactggga aattcttttg tccagctcgg caatcgagct tgctatttcc
     6001 cccagttcct tttcgagctt tgttgtcttg gtttttgttt cttcaagcgt ttttcgtttt
     6061 gcttctgcca cgctgagatt ttttctcagc aattcaaggt ttttcctaca ctcttcccat
     6121 tgcctctctg cgctgttcag atcgtattct gcctgtgcaa gatcagattc gatctggggt
     6181 attctttctg atgtttttga tttgtcttca agcagggctt tttgggctga tgtttcttcc
     6241 tttagcgatt ttaatctcga atcggcaatt tccttgcatg cgccgtattt gtccagctgg
     6301 agtatctggg caagtatctc ccttctttct ttgggttttg cctccatgaa gcgcgcgctg
     6361 tcgccctggg tgatgaatga agaagaagca aagacttcgt atgtaaaccc aagctccgtt
     6421 tcaattgcct ggtcggtttc tggtgtactc tttccgcgaa gatcgttttc gccatcacgg
     6481 gtgatttgcc tgaactggag ccttattgcc cctcccctgt ccattgccct gtcgacaaga
     6541 taggtgttct gtccaatcct gaatgtgagc gagacttcgc aagtgtttgc atcatcattt
     6601 atgacggttt ccctgtcaga tttggttttt gacctgatca gggttggctt tctggcctgc
     6661 ccgaatagtg cccaggtgat ggcatcaagg atcgcagatt tgccggcgcc gttcatccct
     6721 gttattgatg ccacatggaa tgatgaaaaa tccagctggt ggaagcccct gtaggagagg
     6781 aaatctgtta tctctagcga tatcgggacc agattacacc acccttgctt cctgcatgat
     6841 ccgttctgcc tcttcctgta tccttgccct gttggtcttc gcaaagtcat cctggaggga
     6901 atccaggtac gattttacgg cctctacagg cgattcagtc tgggcgatga ctgctttttg
     6961 cgattctctg gcagacggtt tgtaatatgg tgctattatc ttgtcgaaca ttggtaaggt
     7021 atcgagccag gatttgatgg ctggccatat tgaccttgcc tcttccggca gtctgagcct
     7081 gaagatggga agcggcccct tcattgtttt ttcgatttgc tcctggccgc aacctgttgc
     7141 cgcatactca taaggctggc gccagaattc tgatttgagg aattttgcct ctagctcttc
     7201 cttccagtcc agcgcattgt caacagtcaa ttgcctgagt gaccttgagt gttcgacctt
     7261 gcggcatttt ccggatgacc cttctggcgt gaggtcaagc tccatgaaca ttcttgtcct
     7321 ggcaccatcg ggaaagtctc tcctgcttgg ggctccacta tatgccacct gtatttttct
     7381 tacaggcttg ccgtccctga acagcgaatc aggatcaggt gccactgtct cgtttgacga
     7441 gagctcccag tccctgcaag ggtttgttcc gcacaaaatt gatgtctcac catccatgtc
     7501 ctcgtcaagc gatggtgcga caaggtttgg gctgggcatc tgcgggctgt ggtaatggcc
     7561 gagtgcgaca tagccaaatt ttgcctgcga atccgagagt tttgacatca ggtcgtatgt
     7621 ggagattatc acgtcatctg ccaggtagcc ctttacaccc ggcctgagct cggcctcttt
     7681 tatgccgaag tggccaacaa agattgttgg gagtccaggg agggccttcc ttgccaactc
     7741 ctggatgcga tccaggaata ttgttgcggc gagttttttg acctcttccc tgcccctatt
     7801 tttcatttgc tcggaagtga gtatttcgga ccttctcgga tagggcatgg cgaggatctg
     7861 caagttgcct gactttgtgg gtatggttga aatcccgccc attgtttcgg tgtcccctgg
     7921 ttttgagggc tggcaaccca gcacatggca ggcgatttcg gtgccgtctt tttgcgggtg
     7981 caatgccctg aaaacttcga gtgcagaagg caccctcgaa gatgatgagg tttcgtggtt
     8041 gccaggggtc aggatgactg taattgatga atcgacgagg tttcgtattc ttttggcaaa
     8101 ttcggatatt acataattgg ttggccgttc attgtcaaaa atgtctcctg ccacaagaaa
     8161 ggcgtcacaa tcaagtgatt ttgcctgcag gacgagccag tcaagtgttt cgaggtaatc
     8221 cttgatccgg ctgttgattc catcctggtt tgtaacgccg tatgtctcga ccccgaggtg
     8281 gaggtcggca gtgtgcaaaa ccttaatcct gctcatcttc tgcttgctgg ttgggaaaac
     8341 ctatgacgac cgtgtactcg ccttttggtt gtatattttt ttgcctttca agtgcttctt
     8401 ttggcgtgcc cctgaaatac tcctcatgga tttttgtcat ttccctgcat atcaggagct
     8461 gacaatcagg aatgttctgg caaatcgatt ctagcagggc gagcatcctg aaaggggact
     8521 caaaaacaac cgttgtcctg tcctcctggg ctatttgctg gagcgccttg ccaagggcgc
     8581 ctggttttct ggggaggaag tttctaaacg tgaacgtctg ggcaaaaaag ccggaaacag
     8641 aaagcgctgt tgtgatggca gacggcccag ggataggaac cacttttatc ccgtggttga
     8701 ttgccatatc aacaagaatc tggcctggat cggcaagggt cggcactccg gcatctgaga
     8761 cgatggctat attttgcccc atcatgagga tctgtaatgc cttgtttgag gaaatggatt
     8821 cattgtgaat gtgcaatggg aagagttgtg cctttatgcc aaaatgggtc aggagcttca
     8881 gtgttcttct tgtgtcctcg gccatgatga ccgggcactg cgagagtgtt tgtatgagcc
     8941 ttggcgaagc gtcatcgaga ttaccgattg gcgttgcgca aacaaatatt gttccggtca
     9001 ttgctcgtag ttgtccacca gcgtccggag ttttagtctt gctattccca tcgatggcgt
     9061 gccctcgagc aggagcatga agtatttgtc ttttcccttc atgacaagga cagatcctcc
     9121 ctcgccatcg aaggaaatct cggcgatgtt gcttgtgaag ggcatcgtgg tcctgtcggc
     9181 aatggagtac accgatgcaa gatagccgga gagcactcct tgcgagaaac cggtcccttt
     9241 tgtctgacgg aggagcactt tgccctgttc gtcgagaatc aggaagtctt taacaccctt
     9301 caat
//
LOCUS       DAOSRI010000031         9108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7272_length_9108_cov_3.697890, whole genome shotgun sequence.
ACCESSION   DAOSRI010000031 DAOSRI010000000
VERSION     DAOSRI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7272_length_9108_cov_3.697890"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1457)
                     /locus_tag="PK717_01690"
     CDS             complement(180..1457)
                     /locus_tag="PK717_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46411.1"
                     /translation="MKRFLCFASLIVILASCGGEVEEKIDTTLWAGSGGDTMRSSLAA
                     GEIVAPFGKMWETEINGLGVSSVAVALVPPEEEKGRLTAVYRLVIASGNDGAVYGIDF
                     DTGMKMWEKRLEGMTGNPLYYSGKVFISTTKGFWHCLSAWTGDEIWSARYSEKELERP
                     DNATAVVYSGKLYCAHSNGVLFCFDTNKGTELWKKVFKDSIQCSPVVVEDKIILSTYE
                     MNVIALDPNNGNELWNSKIESPVTTTLISDGKLVFAPQAFGVVSAFDVVDGKEAWKIK
                     ACETIAYSPCLTKSGLMAIPDKKAGKIVFVRLSGGTVDSEVKVPWPSSGLVAAGEMVC
                     FAAEDGKVYTVDAAKKQAIESFDFGTKGADNRYKKFGDPCIIQGRLFISDGASKLYMF
                     VPKGFEPTKIENGNTIIPSNNQPKEIVPPKNNP"
     gene            1630..2682
                     /locus_tag="PK717_01695"
     CDS             1630..2682
                     /locus_tag="PK717_01695"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HOR46412.1"
                     /translation="MRKTSIIISCIALVAIAFSAGCGGSVPVSVSSSVPAPYESAPTG
                     TVVTAEYTELAPRIDGVASELDEAWANAQSTIVKVSDSANTYEVTLKAAFDDENFFLL
                     ASWADESMDIGGKPWEVREGGTFKSGTSLPKQDMFALGFEASPIKEFTKQGCSVLCHD
                     NSYMATNDKGEFIDIWTWMSAESGQLGTANDYVVGPLANSVDPKGETFETTSAYSYFP
                     GSLYINKRNARSPEFMQRLTIPYSLMTIGDLSKMVEVPADTTGLPAGLMVPYYIKNPG
                     TGDVACQAKYDDQAKAWTVEFKRKLVTANPTQVQFAHKLEDGGFYLFGLSLFDNQKGV
                     SHVYTTEPVSLEFSGK"
     gene            2692..3765
                     /locus_tag="PK717_01700"
     CDS             2692..3765
                     /locus_tag="PK717_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOR46413.1"
                     /translation="MGEKLLMKGAEAIGEAAIRAGCRHFFGYPITPQNEAPAYMARRL
                     PEVNGTFLQAESEVAAINMLYGCAAAGKRVITTSSSPGISLMQEGLSYIAGSELPVVV
                     MNVVRGGPGLGDIAPAQGDYFQAVKGGGHGDYHLIVLAPSTVQESIDLMQLAFDLADK
                     YRNPVMMLSDGLLGQMMEAVEFPGFVDIEKLPKKPWAADSMQGKRSRNLVNSFDLLPE
                     GLEVMNKKMWAKYADVEKNEVKFEEVETADAEYVFVAYGTVARIIKTVIKDLRSKGHK
                     VGLIRPITLYPFPKKQLLALAENPRVKFFLDVELSFGQMIEDVKLSTEFKKPVHFYGR
                     QGGMLPEPNEISEVMLKLMKEAK"
     gene            3765..4508
                     /locus_tag="PK717_01705"
     CDS             3765..4508
                     /locus_tag="PK717_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR46414.1"
                     /translation="MEQLNHMPTPLMDIPRSYCPGCTHGIIHRLVAEVIEEMDLLQKA
                     IIVLPVGCSTLAVDYFAFDSVQSAHGRAPAVATGVKRALPDRFVMTYQGDGDLASIGA
                     AEMVHAATRGELITTIFVNNGIYGMTGGQMAPTSLMGWKTETSPYGRTAQANGYPIRV
                     SEMLAQLNGPAYIARTDVVSVPGINKTKAAIKKAFTAQVKGLGYSIVEILSTCPTNWG
                     MTPVDALKRVKEETMKYYPLGEFKVAEGL"
     gene            4508..5053
                     /locus_tag="PK717_01710"
     CDS             4508..5053
                     /locus_tag="PK717_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR46415.1"
                     /translation="MAQHELVAAGFGGEVLMMLGKLFAEAGVLEGNYATFLPSYGPAM
                     RGGTANCAIVISNEPIASPVLPHPKVCVVMNLPSLKTFEPLVAPGGILIVNSSLIKEK
                     AKRTDIKTIYVPANEIAEKAGMAKAANIVILGIVVKATGAVKVESTKEAIKQTLGEAK
                     AKYLPINLQVLDAGLNFDING"
     gene            5046..6146
                     /locus_tag="PK717_01715"
     CDS             5046..6146
                     /locus_tag="PK717_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR46416.1"
                     /translation="MDSKRLLKTFLDLVQIPSESGKEKPVTDYLVAKLRSLGLEVWVD
                     DAGSKIGSNSGNIMGRLKGSLPGSIAFSSHQDTVVPGIGIKPQIADGIIRSDGTTILG
                     ADDKAGLACILEMFECIKDKPHPTIEVVFDICEEVGLRGAAQFDTSKLESKYSVILDV
                     DGAPGRINIAAPYQDVCKITIKGKPSHAGIAPEKGISAIVIASKAIASMKLGKIDDET
                     TSNVGIISGGKANNIVPEECKVEMEARSRDEAKLNAQTKHMLDRFEEEAKKVGATIEV
                     SKERVFMGYKFSETDPLPSLVAQAAKDVGLKPQFVRSGGGSVSNIFNAKGLASVVVGV
                     GFENPHSKDEFLRISDLEASANWVIRMVELVK"
     gene            6143..7348
                     /locus_tag="PK717_01720"
     CDS             6143..7348
                     /locus_tag="PK717_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46417.1"
                     /translation="MIRKAVSALIAFFVVLVPFSGVFALKDIQVSFSSGFSTTGYQKT
                     TIEADIVTQFKLVQDNLGHSNTKLNVDVFPSYQSILAKQAKFIDSDTIRVVVTPDYLK
                     KTGSTKPIFYEILSILYPNCLEQTKRILSFYFSSIYQKINLQSFASLCRIVFAEEEIP
                     VSAEAKEISLQIMARAAARIQNIGQNESQARLLEFLQICVKDGIDKAITQLYGDNLRS
                     FVRSAQLPLPPKTGREELVNLVKDYSEYSCMPPKNKVPGDQISLQSHMDEVNIVIFQI
                     ARGNTSELKATLGELSGELKVCRIRDMAWWILCLISVFVVIAFLMLLVRLSKEYGTQR
                     HQKHETTKIIKTVKKMVDSTPAVSTNTEEKNIEHPEVKKRFRGKRVTRIGEESKFVGK
                     KSKKRADNQ"
     gene            7345..8313
                     /locus_tag="PK717_01725"
     CDS             7345..8313
                     /locus_tag="PK717_01725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR46418.1"
                     /translation="MSFSPERRFKSSLLVFALASVLALLVSFSRNEWISVLFSYSVLL
                     GSAYLVYAQSAFMTLVGFKPSPNIRQIVFISFYFLIILIQRTAIKWFSGGEWGDAITA
                     SFIILIIALCERYSLRSFGLTIRNFGRQILGSTISIATFWGCFILAGIFGPIIMGARV
                     MGFGMSFPALDMDLLFSLLYLVLVSFASEIFFRGYIQTKIKGLFGTPVSIIIQAILFM
                     LFNVTTLIWIWHPVGIGETWMLLLQWFLFGIALGLLFKASGSLVVTTLTSLFIRLFLF
                     GGSLVPIAQTSSGVYALPFSEIWSLLLANIVLMIYIATTKTKDNIE"
     gene            8310..>9108
                     /locus_tag="PK717_01730"
     CDS             8310..>9108
                     /locus_tag="PK717_01730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOR46419.1"
                     /translation="MKIAILTSGGDAPGMNAAVRAVTRKALSLGHEVFGIKYGYKGLL
                     DGCIEPLQARDVGGILEMGGTILRSSRSEEFKSEEFQKKAASNIMERGIDYVFAVGGN
                     GTQTGLHALAMQGVKTIGIPSTIDNDLVGTDFTIGFDTAVNTAVWAIDKIRITATSHE
                     RTFIVEVMGRDSGFLALYSGLSCGADTILVPEYKVDLNEVALRVIKSRDKKKHHHIIV
                     LAEGAGHAKWLALEIAERTGIDPYVSILGYIQRGGSPTSFDRLHASKL"
BASE COUNT         2610 a         2163 c         2063 g         2272 t
ORIGIN      
        1 tggtgtggaa gaagatcctt gacctggatt taaggaaaga gaaacagact accataatag
       61 caatcagaag gggggacgag gtactcatcc ccccccgatc caatgcaaac tctcgatagt
      121 gacgacctgc tgattgcaat agggccaaga aaaatcctgc aggaaatatc aaaaaaccgc
      181 tagggattgt tttttggtgg cactatctct tttggctggt tgttgctggg aattattgtg
      241 tttccattct caatctttgt tggctcaaaa cccttgggca caaacatgta aagtttggat
      301 gcgccatcgg atataaagag tctcccctgt ataatgcagg ggtctccaaa ctttttgtac
      361 ctgttatcag caccctttgt tccaaaatcg aaagactcta tggcctgttt ttttgcagca
      421 tcaacggtgt aaaccttccc atcttccgct gcaaagcaga ccatctcgcc tgccgccaca
      481 agccccgatg atggccatgg gacctttact tctgaatcca cagtcccgcc ggacaacctg
      541 acaaaaacta tttttcctgc ttttttatcg ggaatagcca ttaatccact tttggtgagg
      601 catggcgaat aagctattgt ttcacaggct tttattttcc aggcttcttt accatctact
      661 acatcaaatg cagagactac gccaaaagct tgcggagcaa ataccagctt tccatccgaa
      721 ataagcgtgg ttgtgacagg actttctatc tttgagttcc aaagctcgtt gccattattg
      781 gggtcaaggg caataacgtt catctcgtaa gtggaaagga taattttgtc ctcaaccaca
      841 actggagaac actgtatcga gtccttgaaa acttttttcc acaactcggt tcccttattt
      901 gtatcaaaac agaaaagtac gccattcgaa tgggcacaat acagctttcc agaatagacc
      961 acagctgttg cattgtctgg tctttcaagt tccttctcgg agtatcttgc cgaccatatc
     1021 tcatcccctg tccatgcact gaggcaatgc cagaaacctt ttgtggttga aataaagacc
     1081 ttgccactgt agtagagggg gttacccgtc atgccttcaa gtctcttttc ccacatcttc
     1141 atgccggtgt caaaatcaat accatagaca gcgccatcat ttcctgaggc aattaccagc
     1201 ctgtagactg cggtcaggcg acccttttct tcctcaggag ggacaagcgc cacagcaaca
     1261 gagctcactc caagcccatt gatttccgtc tcccacatct tgccaaaggg agctacaatt
     1321 tcacctgccg cgagggacga tctcatggtg tccccaccag aacctgccca cagcgtagta
     1381 tctatctttt cttcaacctc tccgccacat gaggcgagga taacgatcaa ggacgcgaag
     1441 cacaaaaacc ttttcatttt taaacctcct gcattattga aattatgtta aacctttgta
     1501 ttgtcaagcc agaaggctcc ttgactctta tttttctgcg tgataaaaaa ttgatgtgag
     1561 aaacatgagc cttcttctta atgatatttt atggagaggg attttttaat gtaaccaaag
     1621 gagaggtcaa tgaggaaaac cagcattatc atttcgtgca tcgcccttgt agcaatcgcc
     1681 ttttccgcag gttgtggcgg ttctgtgccg gtcagcgttt catcaagcgt tccggcaccc
     1741 tatgaatcag ccccaacagg cactgttgtg actgcagagt acacagagct ggcgccaagg
     1801 attgacggag ttgcttcaga actcgacgag gcctgggcaa atgcccaatc aaccatcgtc
     1861 aaggtctcgg atagcgcaaa cacctatgaa gttactctca aagcagcttt tgatgatgaa
     1921 aatttcttcc ttcttgcatc atgggcagat gagtccatgg acatcggtgg gaaaccatgg
     1981 gaggtcaggg aaggcggaac gttcaaatca ggaacttcac tcccaaaaca agacatgttc
     2041 gctctcggct ttgaagcatc accgataaaa gaatttacaa agcagggctg ttcagtgctt
     2101 tgccatgata actcctacat ggccaccaat gacaaaggtg aattcatcga tatctggaca
     2161 tggatgtcag cagaatcagg tcaacttggc actgcaaatg attacgtggt gggtccattg
     2221 gcaaactctg ttgatccaaa gggagaaacc ttcgaaacta caagcgccta ctcatatttc
     2281 ccaggctcgc tttatataaa caaacgcaat gccaggtccc ctgaatttat gcagagactt
     2341 acaatcccat actctctgat gactattggc gatctttcaa aaatggttga agtacccgcc
     2401 gacactacag gactgccagc gggtctcatg gttccatatt acataaaaaa tcctggcaca
     2461 ggcgatgttg cttgccaggc aaaatatgat gatcaggcta aagcctggac agtcgagttt
     2521 aaaaggaaac tcgtaacagc aaacccgact caggtccagt ttgcgcacaa acttgaagat
     2581 ggaggctttt atctgtttgg tctctcactc ttcgacaacc agaaaggcgt ctcacatgtc
     2641 tacacaacag agccagtctc tctggaattc tcaggaaagt aggttttata aatgggcgaa
     2701 aaacttttaa tgaagggtgc cgaagctatc ggtgaagctg ccataagggc aggttgccgt
     2761 catttttttg gctatccaat caccccacaa aatgaagcgc cagcatacat ggcaaggcgt
     2821 cttccagagg taaatggaac atttttgcag gcagaatcag aagttgcagc cattaatatg
     2881 ctctatggat gcgcagctgc aggcaaaaga gtcattacaa catcatcctc accaggcatc
     2941 tcactcatgc aggaaggact ttcttacata gcaggctcag aattgccagt ggtagtgatg
     3001 aatgttgtta gaggcggccc aggtcttggc gatattgcac cagcacaggg cgattatttc
     3061 caggccgtaa aaggcggcgg acacggagac tatcatctca tagtcctcgc accatcaacc
     3121 gtccaggaaa gtattgattt gatgcagtta gctttcgatc ttgccgacaa gtatcgcaac
     3181 ccggtaatga tgctctccga cggtctcttg ggacagatga tggaagcagt ggaattccca
     3241 ggttttgttg acatcgaaaa gctccccaag aaaccatggg cagccgacag catgcaaggc
     3301 aagaggtcca ggaaccttgt taattcattc gaccttcttc cagaaggcct ggaagtgatg
     3361 aacaagaaga tgtgggcaaa atacgccgat gtcgaaaaga acgaagtaaa gtttgaagag
     3421 gtcgaaacag ccgatgcaga atacgttttt gtggcatacg gcaccgttgc ccgtatcatc
     3481 aagaccgtaa tcaaggacct ccgttcaaag ggacacaaag ttggcctcat aagaccaatc
     3541 accctttacc cgttccccaa gaaacagctc cttgctctgg ccgaaaaccc aagagtaaag
     3601 ttcttccttg atgttgagct gagctttggc cagatgatcg aagatgtcaa actctcaaca
     3661 gaattcaaga aaccggtcca tttctatggt cgccagggtg gcatgctgcc agaaccaaac
     3721 gagatctccg aagtaatgct taaacttatg aaggaggcaa agtaatggaa cagctcaatc
     3781 acatgccgac tccactcatg gacatcccga ggagctattg cccaggatgt acacatggca
     3841 ttatccacag actcgttgcc gaagtaatcg aagagatgga tctcctccag aaggcaatca
     3901 tcgttcttcc agtcggttgc tccacacttg cagtcgatta tttcgctttt gactcagtcc
     3961 agtcggccca tggtcgtgca ccggccgttg ccactggcgt caaaagagcc ctcccggaca
     4021 ggtttgtaat gacctatcag ggagatggtg acctcgcttc aatcggcgcc gccgagatgg
     4081 tccacgcagc caccaggggt gaactcatca ccaccatttt cgtcaacaac ggcatttatg
     4141 gaatgaccgg cggacagatg gccccaacct cactgatggg ctggaaaacc gagacttccc
     4201 cgtacggaag aacggcacag gcaaatggtt atccaatcag ggtttccgag atgctcgccc
     4261 agctcaatgg tccagcctat atagccagaa cagatgttgt ttctgttcct ggaatcaaca
     4321 aaacaaaggc tgcaatcaaa aaagccttta ctgcacaggt caagggcctt ggctattcta
     4381 tagttgaaat tctctccacc tgcccgacaa actggggaat gaccccagtt gatgcactca
     4441 agagggtcaa agaggaaaca atgaagtatt atcctctcgg tgaattcaaa gttgcggagg
     4501 gactctgatg gcacaacacg aacttgtcgc cgccggattt ggcggagagg ttctaatgat
     4561 gcttggcaag ctctttgccg aagcgggcgt tcttgaaggc aattatgcca catttcttcc
     4621 ttcctatggc ccggccatga ggggtggaac agcaaattgc gcaatagtca tctccaatga
     4681 accaatcgct tccccggtac tcccacatcc aaaggtgtgc gtggtaatga accttccgtc
     4741 gctcaagact tttgaacccc tcgttgcccc aggcggaatc ctcatcgtca actcctccct
     4801 cataaaagaa aaagccaaaa ggaccgacat aaagacgatc tatgttcctg caaacgaaat
     4861 agctgaaaaa gcaggaatgg caaaggccgc aaacatagtt attcttggca ttgtggtcaa
     4921 ggcaactggc gcagtcaagg tggaatcaac aaaagaagct atcaagcaaa cccttggtga
     4981 agcaaaggca aaatatctgc caatcaacct gcaagtcctc gatgcaggcc tcaattttga
     5041 catcaatgga tagcaaaaga ctcctcaaaa cttttctcga ccttgtccag atcccgtctg
     5101 aatcaggaaa agaaaagccg gtgaccgact acctcgtggc caagttgaga tcactgggcc
     5161 ttgaagtgtg ggtagacgat gccggaagta aaatcgggtc caacagtgga aacatcatgg
     5221 gcaggctcaa agggagcctg cctggttcca tagcgttctc atcacaccag gacacagttg
     5281 ttcctggcat tggcataaaa ccacaaattg ctgatggaat aatacgctca gatggaacaa
     5341 caatcctcgg tgccgatgac aaggccggtt tagcctgcat cctggaaatg tttgaatgca
     5401 tcaaagacaa accacaccca acaatagagg ttgtctttga tatctgcgaa gaggtaggcc
     5461 taaggggcgc cgcacagttt gatacgtcaa aactggaaag caaatattcg gtaatcttgg
     5521 atgttgatgg tgcacccgga agaataaaca ttgctgcccc ataccaagat gtttgcaaga
     5581 ttacgatcaa gggcaaacca tcacatgcag gaatcgctcc tgaaaagggc atctcagcca
     5641 ttgtcattgc ttcaaaagca atagcgtcaa tgaagctcgg caaaattgat gacgaaacaa
     5701 cttcaaacgt tggcatcata agtggcggca aggccaataa catcgtcccc gaggaatgca
     5761 aagttgaaat ggaagcaaga tcaagggacg aagctaaact gaacgcccaa accaagcaca
     5821 tgcttgacag gtttgaagaa gaggccaaaa aagttggcgc tacaatagaa gtctcaaagg
     5881 aacgggtttt tatgggatat aaatttagcg aaacagaccc gcttccgtcc cttgtcgccc
     5941 aagccgccaa agatgttggt ctaaaacccc aatttgtgcg ctctggtggc ggatcagttt
     6001 ccaatatctt caatgccaaa gggcttgcat ccgttgttgt tggtgtcggt tttgaaaatc
     6061 cacattcaaa agatgaattt ttacgaattt cagatcttga ggcatcagca aattgggtca
     6121 tcaggatggt tgagctagtt aaatgataag aaaagcagta tcggctttga ttgcgttttt
     6181 tgtagtgctg gtcccatttt cgggtgtatt cgcactcaaa gatatacaag tgtctttttc
     6241 gagcgggttt tcaaccactg gctaccagaa aacaacaatc gaggccgata ttgtaactca
     6301 attcaagctg gtacaggaca accttggaca ctccaatacc aagttgaatg ttgacgtttt
     6361 tccaagctac cagtccattt tggccaaaca ggccaaattt attgactctg acacaataag
     6421 ggtcgtcgtc acgccagatt acctcaaaaa aacaggttcc acaaaaccca ttttttatga
     6481 aatcctttca atactctatc caaattgtct ggagcagaca aaaaggatac tttctttcta
     6541 tttttcatca atttaccaaa aaataaacct gcaatctttt gcgtcacttt gcaggatagt
     6601 ttttgctgaa gaagaaatcc cggtttctgc ggaagcaaaa gaaatctcgc ttcagatcat
     6661 ggcaagggca gccgcaagaa ttcaaaatat tggacaaaac gagtcccagg caagactttt
     6721 agaatttctc cagatatgcg tcaaggacgg gatagacaag gcgataacgc agctttacgg
     6781 agacaatctg cgaagttttg taagatcagc acagctccca ttgcccccca aaacaggcag
     6841 ggaagaactt gtcaacctcg tcaaagacta ctccgaatac tcctgcatgc cccccaaaaa
     6901 taaagtccca ggcgaccaaa taagtctgca atcccacatg gacgaagtaa acattgtaat
     6961 tttccagatc gcaagaggca acacaagcga gctaaaggca acgcttgggg agctctccgg
     7021 ggaactgaag gtttgcagaa taagggacat ggcatggtgg atactttgct taataagtgt
     7081 ttttgttgtt atagctttcc ttatgctcct tgtgcgtctc tccaaggaat acggaactca
     7141 aaggcaccaa aaacacgaaa ccacaaaaat catcaaaacg gtcaaaaaaa tggttgattc
     7201 aacacctgct gtttcaacaa ataccgaaga aaaaaacatc gagcatcccg aagtgaaaaa
     7261 aagattcaga ggcaagaggg tcacaaggat tggggaagaa tccaaatttg taggcaagaa
     7321 atcaaagaaa agggcggaca atcaatgagc ttttcccctg aaagaagatt caagtcatcc
     7381 cttttggttt ttgctcttgc atcagttctt gccctgctgg tgtctttttc cagaaatgaa
     7441 tggataagcg ttcttttttc ctactcggtc ctgctgggaa gcgcatatct ggtttatgcg
     7501 caatctgcat tcatgacgct tgtgggattc aagcccagcc cgaacattag acaaatcgtt
     7561 tttataagct tctatttctt gatcatcctc atccaaagaa cggcaataaa atggttctct
     7621 ggcggtgaat ggggcgatgc gataacagcc tcgtttatca ttctgattat tgccttgtgt
     7681 gaaagatact cgctcaggtc attcggcctc acaataagga attttggaag acaaatcctt
     7741 ggttcgacca tatcaatagc aaccttctgg ggttgcttca tacttgctgg tatttttggc
     7801 ccgatcataa tgggtgcaag ggtcatgggt tttggaatgt ctttccctgc actcgatatg
     7861 gatttgttgt tctccttgct ttacttggta ctggtcagtt ttgcatccga aatctttttc
     7921 aggggttaca tacagacaaa aatcaaagga ctttttggca cccccgtttc aataatcatc
     7981 caagccattc tcttcatgct ctttaatgtc actacactga tctggatctg gcatccggtt
     8041 gggattggcg agacatggat gcttttgctc cagtggttcc tttttggcat cgcattgggg
     8101 ctcttgttca aggcatcagg aagcctcgtt gtaacgaccc tgacaagcct ttttatcagg
     8161 ctgtttcttt ttggtggcag ccttgtcccg atagcacaaa catcttcagg agtatatgcg
     8221 ctgcccttta gcgagatatg gtcactgtta cttgcaaata ttgtcctcat gatttatatt
     8281 gccacgacaa aaacaaagga caatatcgaa tgaagattgc cattttaaca tcgggcgggg
     8341 acgccccagg gatgaatgct gcagtaagag cggtcacaag aaaggctctt tctcttggcc
     8401 atgaagtttt tggaataaag tacggataca agggcttact tgatggctgc attgagccac
     8461 tccaggcaag agatgtaggc ggaatactgg aaatgggcgg aacaatatta cgttccagcc
     8521 gttccgaaga attcaagtca gaagaatttc aaaaaaaggc cgccagcaac attatggaaa
     8581 gaggcatcga ctacgtgttt gctgtcggtg gaaatggcac tcaaacaggc ctccatgcac
     8641 tggcaatgca gggcgtaaaa acaattggca taccttcaac aatcgataat gacctcgtgg
     8701 gaacagactt taccattggt tttgataccg cagtgaacac tgccgtttgg gcgatagaca
     8761 aaatcaggat cacagccaca tcgcacgaaa gaacctttat cgtcgaagtc atgggaagag
     8821 actctggttt cctggcactt tatagcggcc tttcctgtgg cgcagacacc atccttgttc
     8881 cagaatacaa ggttgatctg aatgaagtgg ctttgagggt cataaaaagc agggacaaga
     8941 agaaacacca ccatataatc gtgcttgccg agggagccgg ccatgcaaaa tggctagcgc
     9001 tggaaattgc ggaacggacc ggcatagatc cctatgtctc aatccttggt tacatccagc
     9061 gaggcggaag cccaacatca tttgacagat tgcacgcctc aaaacttg
//
LOCUS       DAOSRI010000032         9090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7290_length_9090_cov_4.119757, whole genome shotgun sequence.
ACCESSION   DAOSRI010000032 DAOSRI010000000
VERSION     DAOSRI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7290_length_9090_cov_4.119757"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1307)
                     /locus_tag="PK717_01735"
     CDS             complement(33..1307)
                     /locus_tag="PK717_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR46420.1"
                     /translation="MKIALRQGLYFATVFVFLCTLSSCMSLPVPKIEFSVETIPPEAS
                     IYVDGTFYGFTPKVIEVSKVSSVTLELDGYETYRFSATQYSGDRGFVRATLNKLYQVH
                     LAYSPEGSEIWENGKLLGKTPLTIYRPEGRISLRIKHEYFHDLLDTFDIVEVKLRARV
                     LKAKTRYFPDTVCSITSSPQGAEVLHFGFEGGKIISDAKVIGTTPLNITNAELSKISK
                     EHLVVIRKEGFLPSIMVFNGSIEAHFELGPKEKDPHPAMPKNGVTQVQYKMGRITQKN
                     VTLDVISKNGNVRIINPEIGVDVEDMKTPLSKNSGAAFWGNFFFSVRYQESDGDTFYL
                     VFDRKNRKRFRIEPSNFFCETPINGDIGYKQRVPITNSGFEKFSDLSIADNGKNIYIF
                     AKPYGGTCPYMIFKSDQSLQNMIFIGLLDSPQ"
     gene            1430..2833
                     /gene="purF"
                     /locus_tag="PK717_01740"
     CDS             1430..2833
                     /gene="purF"
                     /locus_tag="PK717_01740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR46421.1"
                     /translation="MHEECGVFAVAGHPDAAYLTYVGLLALQHRGQEAAGMVTFREPD
                     VFTCHKDLGLVVDVFSKGAVADLSGKYALGHVRYSTKGSNTRENAQPHKAGHLWVASN
                     GDVVNYTSQRIFLEQNDITFLSTNDGELLAKSILFNSQRKGGLVPGILQLMNHVHGTY
                     SAGLLTSNKVMAFRDPAGIRPLVLGQKDGCGVLSSETCALDAIGAKYIREVAPGEIVD
                     LETLATLGTSKNAMNPGRICIFEYIYFARPDSRIRNNLLWQARYEMGRQLAIEDPEKN
                     SDIVMPVPETGNPCAAGFAAQSGLPLIPAIVKNHYVGRTFIKPEQSQRMEAVRIKLNV
                     MRDLVQGKNVMVMEDSIVRGTTTKILVQKLKDAGAKRVSLAISSPPIKFPCFYGIDTG
                     VRKDLIAADLTVDQVREYIGADQLYYLSLPGLLKACKGELPQFCTACLNGDYPIPVNE
                     DGSLPGKAEWENGRQGV"
     gene            complement(2834..3841)
                     /locus_tag="PK717_01745"
     CDS             complement(2834..3841)
                     /locus_tag="PK717_01745"
                     /inference="COORDINATES: protein motif:HMM:NF037020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46422.1"
                     /translation="MAYCGIVRNMGRFGSALTIFVLCTGILSCSGGKKSEEIGASQKA
                     LDAAFQKISAVTEEPKKGRLIIHFITPTSIASKEAATYLDKMEPELEKQGFRTVYICS
                     PEYEFLKIGNELSRQVSELKPKNPVVFDMDYTIWAAFGAIKYGYTSLVIDGEIKNSTR
                     EFLGQSTLEKLLGNSFRAISKTANEMPFTPSRIVKCGYLDGRIGNCKGTEIERVYEFT
                     DPRSYTEFTPYLEGKWYFGPEMAWHSDDKTGRLSISFAGKEVYMFAKSQSDKSFPVQV
                     WVDGLLPDKNYYGSDMKQGRVTISDGKIYNVLKNLPPANHTLTIEIQSKDCAIFSLEF
                     R"
     gene            complement(3913..4254)
                     /locus_tag="PK717_01750"
     CDS             complement(3913..4254)
                     /locus_tag="PK717_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46423.1"
                     /translation="MPREIVFGSSPGADAPPNQNKLAGSSCDPQPLTRKNIMIPRMAI
                     PIAMMVMFFFVMPAILVLNLDFAIKHPIFKIPIRTSLSVLSELKRKTSPEKFFYVQIN
                     IVPNNISSLAG"
     gene            4256..6718
                     /locus_tag="PK717_01755"
     CDS             4256..6718
                     /locus_tag="PK717_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46424.1"
                     /translation="MTPPDQKLFEFQVEGLPKGVTFEERREDLSSKSYMSFLVATIPA
                     DTKKGIYPIKITYKNASFSKSFESEFVIGDRAESQDNIYGGIGRSGQLPAISTDDLVV
                     SNFVNLEKGITSPPCIVGNKTYLGLQNGKVVALDDKLGKIFEVTVSEDPVVAVQATEK
                     YLVGCDSRGNIASFDLSQKEPSQLAAYKLDGKASSPPTIVAEDKILVGSTDGLLGLFS
                     LPNLEPQWKVNLPGPVIGNVSCQIYKSENDLKGYIFAACDDSNIYYHDLGSKLISRFQ
                     MDLSSKFAPIVVGDRVLFSDSENFIQLSDSFGRKIWSTNVEDIQLWQPISNSGKAYFF
                     GKKKIIALDMKDGSVSFSVDSPGEIISQPVFIGETLCLPLQGQLSFMSGDLTPKSSIN
                     LGDLVSGWPVPFEDGLLVAQQSGGVYLFAKGNPGSSIPVVKFNMKDVVFSQGLSNQFH
                     NSYTNTKLPKTLKLKWEKDGEYAPVLTTKTRAYLYNITKKVFSCVSIDDGKEIWQFPS
                     EAYPAVYHTLGYNETPMCLSENGLYLGTVAGVMLVDPDTGRLLRSSNVSGIPQADDSA
                     VVVTNGKKLSVCDKNLKTLWVANGNFVSSNVIILGKSVIAISRDEVEDFSKSKAFVAI
                     YNKLTGKVEWSKKDTSISYCVRTITDGKRLLASTGSGLWMMNIGERKVYGASRYAPTV
                     ETIYAMPNGDIWFKEMAGIHFKLNLKDGNISPVIEPPQDKPIYMAFGLTYMDSESFVG
                     ICAPLPDPKETEQSQESIDVNKADFYLVTTSVKTGNNISSVKIGKSFDAQFALSYGEK
                     KVFVSTFGSDKGYNPKLSVWGD"
     gene            complement(6701..7357)
                     /locus_tag="PK717_01760"
     CDS             complement(6701..7357)
                     /locus_tag="PK717_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOR46425.1"
                     /translation="MKFENREEAGKQLASKLPQEFRGVSVVYGIALGGIVCSLPVANS
                     LKVPLEPAFVSKLPLPWNQESAFGVVISDGNVILDEGLYRQVGLSYMDVQRIARAKLA
                     EIKRHEQNIRFDNPVEDLKDKEVLLVDDGLATGYTCLGAAKWLLGKKPAKVFIATPIA
                     HHSAIELLESNGFKTFSLFTSTNKVFAVGSYYKDFEQVGDKIARTTIKENRDYLVSPD
                     "
     gene            complement(7354..8100)
                     /locus_tag="PK717_01765"
     CDS             complement(7354..8100)
                     /locus_tag="PK717_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46426.1"
                     /translation="MKRFWLFLVVAAIILAGVNPRPTVRCAQPQERVTIRPSTSIAPK
                     GTIVRLTAQIKNEGNEETPEGTLIVEVGAEFEIKDTSPPAAIAGNKAIFRFNQLDAGQ
                     CFTGYVNVEIVTKIAIVAHPMVCKVSSDRPQGLIKSNCVILLWAPSEYPPLTAELRES
                     GREKGTVRFNLKIIGGFPPYEFYFDWGDGPNGGTGDKKGGLPQEGQHTIEHTYLKPGE
                     YILNGFINDWLGKQVVLRRRLYIIPWEDKP"
     gene            complement(8101..>9090)
                     /locus_tag="PK717_01770"
     CDS             complement(8101..>9090)
                     /locus_tag="PK717_01770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR46427.1"
                     /translation="MINPLDGMTTKEARMAGVEPFIIKQNPYQLAKLMRDLDLDLIHT
                     HGVRANFLGRMASKITGIPNICTMHSDSRLDYASSVKERAVWYADNVFNGMADAFIGV
                     SNDMSENLAKRGIPREKIFTVHNGIDLSCVKSDADPEKTKEKFGIPKNIRLIGTVGRL
                     VEVKGHADMVAALPRILDKIPDAGMLIVGDGRCLEPLREQAEKLKVNDRVFLPGRIKD
                     PFDLMKACDIGCFPSIAEAIGLALLEFMALEVPVVATRVGGIPEVLTKPEYGVLVPPS
                     SPKELSGAIISLMGDRKQMEHVGKQGRERVEQEFTTGKMIEKTQEVWEIFARK"
BASE COUNT         2320 a         2014 c         2100 g         2656 t
ORIGIN      
        1 aaacaaattt cggtgcaggt gatgatggat tgctattgcg gggaatccag gagcccgatg
       61 aaaatcatgt tttgcaagga ctggtcggat ttgaatatca tgtaagggca tgttccgcca
      121 tagggctttg cgaaaatgta gatgtttttc ccgttgtcgg caatcgatag gtccgagaat
      181 ttttcaaaac cagaattggt gatggggact ctttgcttgt agcctatgtc gccatttatt
      241 ggtgtttcac aaaagaaatt tgatggctct atcctgaacc ttttccggtt tttcctgtca
      301 aaaaccagat aaaaggtatc tccatcagac tcctggtatc ttacgctaaa aaagaagttg
      361 ccccagaaag ctgcgccgga gttttttgag agaggagtct tcatgtcttc tacgtcaacg
      421 ccaatctcag gattgattat cctcacgttg ccgttcttgg agatgacatc gagagtgacg
      481 tttttttggg ttatgcgccc cattttgtac tgcacctggg tgacgccatt tttcggcatg
      541 gcgggatgag ggtctttttc ttttggccca agctcgaaat gtgcctctat gcttccgttg
      601 aacaccataa ttgatggcaa gaatccctct tttcttatta ccacaaggtg ttccttcgag
      661 attttggaaa gttcggcgtt tgtaatattt agtggtgttg tgccgatgac ttttgcatcc
      721 gaaatgattt tgccaccttc aaaaccgaaa tggagcactt cggcaccctg ggggcttgag
      781 gttatagaac aaacagtgtc tgggaagtac cttgtttttg ccttcaggac acgcgctctc
      841 agtttgacct ccactatgtc aaacgtgtct agaaggtcat ggaaatattc gtgctttatc
      901 ctcagtgaaa tcctgccctc tggccgatat atcgtgagtg gcgttttgcc cagaagtttg
      961 ccattctccc atatttcgct gccttcaggc gagtacgcga ggtgcacctg atagagcttg
     1021 tttagcgtcg ctcttacgaa gcccctgtcc ccactgtact gtgttgccga gaacctgtag
     1081 gtttcgtagc catcaagctc gagcgtaaca gatgaaactt ttgaaacctc gattactttt
     1141 ggggtgaacc cgtagaatgt gccatcaaca tatattgaag cctctggtgg gatggtttcc
     1201 accgagaact cgattttggg gaccggcagg gacatgcacg aagagagcgt gcacaaaaac
     1261 acaaacactg tggcaaaata taggccctgt cgcaacgcaa tcttcattga tgaacccaag
     1321 ttaccattcg aaggaccact tttcaaggta accatttcct acctttttgt ttactagctg
     1381 accttgactt aaaagcaggt tgtgagaaat tttacttgga ggtgcgtgaa tgcacgaaga
     1441 gtgtggagta tttgcagttg cgggtcatcc tgatgctgcc tatctgacct atgttggttt
     1501 gcttgccttg cagcacaggg gccaggaagc tgctggaatg gtcactttca gggaaccaga
     1561 tgttttcaca tgccacaaag accttgggtt ggtcgtggac gttttctcga agggtgccgt
     1621 tgccgacctc tcagggaagt atgcacttgg ccatgtaagg tattccacaa aaggctccaa
     1681 cacccgcgaa aatgcccagc cccataaggc cggacacctc tgggtagcat caaatggtga
     1741 tgtggtcaat tacacatccc aacgcatttt cctggaacaa aacgacatca ccttcctctc
     1801 gacaaacgac ggagagctcc ttgcaaaatc aatccttttc aacagtcagc gcaagggtgg
     1861 gcttgtcccg ggcattctcc agctcatgaa ccatgtccac ggcacatact cggctggttt
     1921 gctcaccagc aacaaggtga tggcattcag ggacccggcc ggaataaggc cacttgtcct
     1981 tggccaaaaa gacggatgtg gtgtgctttc atccgaaacc tgtgcgcttg atgcaatagg
     2041 tgcaaaatat ataagggaag tcgcccccgg agagattgta gacctggaaa cgcttgcgac
     2101 ccttggcaca tcaaaaaacg caatgaatcc tggaagaata tgcatctttg agtacatcta
     2161 ttttgcacgt ccagactcca gaataagaaa caacctgctc tggcaggcaa ggtacgagat
     2221 ggggcgccag cttgccatcg aggacccgga aaaaaactct gatatagtca tgcctgttcc
     2281 tgaaacagga aacccgtgtg ccgcaggctt tgccgcacag tcaggacttc cgttgatacc
     2341 agccattgta aaaaaccact atgttggcag gacattcatc aagccggagc agtcccagcg
     2401 catggaagca gtcaggataa agctgaatgt gatgagggac cttgtacagg gcaaaaacgt
     2461 catggtgatg gaagattcga tagttagggg aacaacaacc aagatactgg tccagaaact
     2521 aaaagatgct ggggcaaaaa gggtttctct ggcaatatcc tcaccgccaa ttaaattccc
     2581 gtgcttttat ggcattgata caggagttag aaaagacctt attgccgcag acctgaccgt
     2641 tgaccaggtc agggagtata taggagctga ccagctttat tacctctcgc tgcctggcct
     2701 tttaaaagcc tgcaagggtg aacttccgca gttttgcact gcatgtctca atggtgatta
     2761 tccaattccg gtcaatgaag atggttcgct ccccggaaag gccgagtggg aaaatggcag
     2821 gcagggcgtt taactagcgg aactccagac tgaatattgc gcaatccttt gattgtattt
     2881 ctattgtcag ggtgtgattg gctggtggaa ggtttttgag gacgttgtag atttttccgt
     2941 ctgaaattgt tactctgcct tgcttcatgt cggagccgta ataattttta tcgggcaaca
     3001 ggccatcaac ccatacctgt acaggaaaag acttgtcaga ttgtgatttt gcgaacatat
     3061 aaacctcttt gccagcaaag gatatggaga gcctccctgt cttgtcgtcg ctgtgccagg
     3121 ccatttctgg gccaaagtac cattttcctt cgagatatgg tgtaaattcc gtgtaggacc
     3181 ttggatcagt aaactcgtag acgcgctcta tttcggtccc tttgcaattc ccaatcctcc
     3241 cgtctagata gccgcatttt acgatccttg aaggagtgaa tggcatttca tttgcagttt
     3301 ttgaaatggc cctgaaactg tttccaagga gtttttccag cgtggactga ccaagaaact
     3361 ctctcgtgga atttttgatt tctccatcaa taaccagtga cgtgtaccca tattttatgg
     3421 caccaaaggc cgcccagatt gtatagtcca tgtcaaagac gacggggttt ttcggcttaa
     3481 gttcgctcac ctgacgtgaa agctcattac caatcttcag gaattcatat tccggagagc
     3541 agatgtacac agttctgaag ccctgttttt cgagttcagg ttccatcttg tcgaggtatg
     3601 ttgccgcttc ttttgatgcg attgatgttg gcgtaatgaa atgtattatt aatcgacctt
     3661 tttttggttc ttcggtcact gctgatattt tctggaaggc cgcatccagg gctttttgcg
     3721 aagctccaat ctcttcggac ttctttcctc cagaacatga aagaatacca gtgcatagaa
     3781 caaaaatagt caatgccgac ccaaatcttc ccatgtttct aactatacca cagtatgcca
     3841 tttcatgcca tttatacatc catattgaca tagctgtcag gtttgcctga agaaagatag
     3901 attgcctgga ggttaacctg ccagtgatga aatgttgttg ggaacaatat ttatttgtac
     3961 atagaaaaat ttttctgggc tggtttttct ttttaattcg gaaagaaccg acaggctagt
     4021 cctaattggt attttgaata tggggtgttt tattgcaaaa tcgaggttca aaactagaat
     4081 agcaggcatg acaaagaaga acattaccat catggctatt ggaattgcca ttctaggaat
     4141 cataatattt tttcttgtaa gaggctgtgg gtcgcaagag gaaccggcta gtttattttg
     4201 gtttggtggt gcatcggctc ctggagaact gccgaaaaca atctctcttg gcaagatgac
     4261 tcctccggac cagaagcttt ttgagttcca ggtcgagggt ttgccaaaag gtgttacttt
     4321 tgaagaaaga agggaagacc tcagctcaaa atcatacatg tcttttcttg tggctaccat
     4381 tcctgccgat acaaaaaagg gtatctaccc gataaaaata acatacaaga atgcttcatt
     4441 ttcaaaatcg tttgagtctg aatttgtgat cggtgataga gcggaatcac aagataatat
     4501 ttatggtggc attggaagat ctggtcagct tcctgcaatt tcgactgatg accttgtagt
     4561 ttctaatttt gtaaatctcg aaaaaggaat tacctctcct ccttgtattg ttggcaataa
     4621 aacatacctc ggtcttcaaa atggcaaagt ggttgccctt gatgacaaac ttgggaaaat
     4681 ttttgaggtg actgtatcgg aggatcctgt tgttgctgta caagctacag aaaaatatct
     4741 tgttggctgt gattccaggg gaaacattgc gtccttcgat ctttcacaaa aagagccatc
     4801 ccagcttgcg gcatacaagc ttgatggaaa agcatcctca ccaccaacaa ttgttgcaga
     4861 ggacaaaatt cttgttggct cgacagatgg tcttcttggt cttttctctc ttccaaacct
     4921 ggaaccgcag tggaaggtga atcttccagg tccagttatc gggaatgtgt catgtcaaat
     4981 ttacaaatct gaaaatgatc taaagggtta tatttttgct gcatgtgacg actcaaatat
     5041 ttactatcat gatctaggta gcaagcttat cagccgtttc cagatggatc tgtcatccaa
     5101 atttgctcca atagtagtag gtgacagggt cctcttttct gattcagaaa attttatcca
     5161 gttgtcagac tcttttggta ggaagatttg gagcactaat gttgaagata tacagctctg
     5221 gcagccaatt tcaaactctg gcaaggcata tttttttgga aagaaaaaaa ttattgcttt
     5281 ggacatgaaa gatggcagcg tttctttctc tgtggattca cccggagaga taatttcaca
     5341 accagtgttt attggtgaaa ctttgtgctt gcccctgcaa ggtcaattgt catttatgtc
     5401 tggtgatttg acaccaaaat caagcattaa ccttggggat ctggtttctg gctggccggt
     5461 tccttttgaa gacggtctac ttgttgcgca gcaaagtgga ggagtttatc tttttgccaa
     5521 aggaaaccct ggcagttcga tacctgtagt caaattcaat atgaaagatg ttgtgttttc
     5581 ccaaggattg tcaaatcagt ttcacaactc ctacacgaac acaaaattgc ccaaaacgct
     5641 caagttgaag tgggaaaaag atggcgagta tgcgcctgtg cttacaacca agaccagagc
     5701 gtatctttac aatatcacaa aaaaggtttt ttcatgtgtt tcaatagatg atggcaagga
     5761 aatatggcaa tttccttcgg aagcatatcc tgcagtttac catactctag gatataacga
     5821 aaccccaatg tgtctttccg agaatggtct ttatcttgga acggtggctg gcgttatgct
     5881 tgttgatccg gacacaggaa ggttgcttag gtcttcaaac gtcagcggaa taccacaagc
     5941 agatgattct gctgttgttg taacaaatgg caaaaaactt tctgtttgtg acaaaaacct
     6001 aaaaactttg tgggttgcaa atgggaattt tgtttcttca aatgtcataa ttcttggcaa
     6061 atcggtgatc gcaatttcca gggacgaagt tgaggatttt agcaaaagca aggcatttgt
     6121 tgccatatac aataaattga ccggtaaggt agagtggtca aaaaaagata cgtccatttc
     6181 ctattgtgtg cgaaccatca cggatggaaa aaggcttctt gcttcgactg gttcgggtct
     6241 ctggatgatg aatattggag aaagaaaagt ttatggtgca tcacgttatg cacccactgt
     6301 cgaaacgatc tatgccatgc caaatggcga tatctggttt aaagaaatgg ctggaataca
     6361 tttcaaactc aatctcaaag atggcaacat atcaccagtc attgagcctc cacaagacaa
     6421 accaatttat atggcatttg gcctaaccta tatggacagt gaatcttttg ttggtatttg
     6481 tgccccactt cctgatccca aggaaacaga gcaatcacaa gaatcgattg atgtgaacaa
     6541 ggcagatttt tatctggtta caacgtctgt aaaaactggt aacaatatct catctgtcaa
     6601 aattgggaaa agctttgatg cgcaatttgc cctgtcttat ggtgagaaaa aagtttttgt
     6661 atccaccttt ggtagtgata agggatacaa cccaaaacta tcagtctggg gagactaggt
     6721 agtccctgtt ttccttgatt gtggttcttg ctattttatc gcctacttgc tcaaagtctt
     6781 tgtaatagga accaacagca aacaccttgt ttgttgatgt aaacagggaa aaggttttaa
     6841 atccatttga ttcaagcagt tctattgcac tatggtgtgc aataggggtt gcaataaaaa
     6901 cttttgccgg ttttttgcca aggagccatt ttgccgcacc aaggcaggtg tagccggttg
     6961 caagcccatc atcgacaagc agaacctctt tgtctttcag gtcttcgaca ggattgtcaa
     7021 acctgatgtt ttgctcgtga cgcttgatct cggcaagctt ggcccttgca atgcgttgca
     7081 catccatgta tgaaagcccg acctggcgat atagaccttc gtccaggatc acgtttccat
     7141 ctgaaatgac cactccaaag gccgattcct ggttccaagg cagtggaagt ttggaaacaa
     7201 atgccggttc aagcggaact tttagtgaat tggcaactgg tagcgaacac acaattccgc
     7261 caagggctat tccatacaca acagagaccc ccctaaattc ctgtggcagt tttgatgcaa
     7321 gctgttttcc agcctcttcc ctgttttcga atttcacggt ttgtcctccc aagggattat
     7381 gtagagcctt ctcctcaaaa caacctgttt tccaagccag tcgttaataa aaccgtttag
     7441 gatatattcc cctggtttaa gataggtatg ttctattgtg tgttggcctt cctggggcaa
     7501 accacccttt ttgtcccctg ttccaccgtt cgggccatcg ccccagtcga aatagaactc
     7561 gtagggtggg aaaccaccga tgatcttgag gttgaacctt actgtaccct tttccctgcc
     7621 agattctcta agttctgcag taaggggggg gtattctgat ggcgcccaca gaagtatgac
     7681 gcaattgctt tttatcagcc cctgaggtct atcagacgag actttgcaga ccattggatg
     7741 tgcgacaatt gcaattttgg tgacaatttc gacatttaca tatccagtga agcattggcc
     7801 tgcatcgagc tgattaaatc tgaaaatggc tttgtttcca gctattgccg ctggcgggga
     7861 cgtgtctttt atttcaaatt cagccccgac ttcaacaatc aaagttcctt ctggtgtttc
     7921 ttcattgcct tcgtttttta tttgcgcggt caatctgaca attgtccctt ttggggcgat
     7981 tgatgttgaa ggccttattg tgactctttc ctgtggctgg gcgcagcgta cggttggccg
     8041 ggggttaact ccagcaagga tgatcgccgc cacaaccaga aaaagccaga atcttttcat
     8101 ttattttctt gcaaaaatct cccagacttc ctgggttttt tcaatcattt tcccagttgt
     8161 gaattcctgt tcaacccttt ctcttccctg tttgcctaca tgctccattt gcttcctgtc
     8221 acccatgagt gatattatag caccggaaag ttcttttggg ctggatgggg gaacgagtac
     8281 cccatattcc ggttttgtca ggacttcagg gatgccgccg acccttgttg cgacgactgg
     8341 cacctcaagc gccatgaact ccagtaaggc aagtcctata gcttctgcaa tcgacgggaa
     8401 acagccgatg tcgcatgcct tcatgaggtc aaacgggtcc tttatcctgc ctggcaggaa
     8461 caccctgtca ttgactttca gtttttctgc ctgctccctg agcggctcca gacacctgcc
     8521 atcgccaacg atcagcatgc cggcgtctgg tatcttgtcc agaattcttg gcagcgcggc
     8581 aaccatgtct gcatggcctt tcacttcgac gagtcttccc acggtcccta tgagccttat
     8641 gttttttgga ataccaaatt tttcttttgt tttttcaggg tctgcgtctg attttacaca
     8701 ggagaggtca attccgttgt gcacagtgaa tatcttttcc cttgggatgc ccctttttgc
     8761 aaggttctcg gacatatcgt ttgacacgcc tatgaaggca tcagccatgc cattgaacac
     8821 attgtcggca taccagactg ccctttcctt gactgacgag gcatagtcca gcctagagtc
     8881 cgagtgcatc gtgcagatgt ttgggatgcc ggttattttt gatgccattc taccaaggaa
     8941 gttggccctg acgccatgcg tgtggatgag gtcgaggtcg aggtccctca tgagctttgc
     9001 cagctggtat gggttttgtt ttatgatgaa tggctcaacg ccggccattc tggcttcttt
     9061 tgtggtcatg ccatcgagcg ggttgatcat
//
LOCUS       DAOSRI010000033         8982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7395_length_8982_cov_4.812591, whole genome shotgun sequence.
ACCESSION   DAOSRI010000033 DAOSRI010000000
VERSION     DAOSRI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7395_length_8982_cov_4.812591"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="PK717_01775"
     CDS             complement(<1..2274)
                     /locus_tag="PK717_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOR46428.1"
                     /translation="MTSRSVIRIMTVLMVCASILAPSFSVISPGTVQAAIKPSYQITA
                     YTIAMSQNLKDNTTDIVCEIEFKAFQDIKNVYLYMDPIFKVKSVKGLSDFIQEKDLLI
                     IPVQGKKDKTFTISINYVARLAGTEYPGRAWNYVGQDGIYFFQWWYPVTLFTIAAGYS
                     DVISMNITANLPIGWKAASSDIISEQISKDKAFNEIKIQSRDPSFFYHFIAGPYEILK
                     IEDPKKVISKTTFFGAKDKLDRGKEISSAIFDLLAYYESVFGTPAPKNYMVIQMPDKF
                     KTVIAERASLFIPTAAFAKVEEKDKKDKKILDLADPEYLAERVASSWWGGTVFGVGPE
                     AEFLNTSLSTYSGILYIGQKLGESKMIEMLRKARVEYFEKVQPKDEKPLSSITQEDKL
                     DIYFRKKGPLVFHMLRQVIGEASFFKGLKNYAGKFAGKFATISDISQELSKASGMKLD
                     WFFEQWVNQAGRLTYNIDFVLTPGKNPYKYKITLESKTPFKMPFKIDVIMTDRTVESF
                     DWDLNDPDMEKILSSKKEPVGARIHNPEGYILTDESIVNTLTKGPISNFFYLGNFIIA
                     EGTWQGNELMVKAADARAQFYKKMLKKYYNLDVPIVLDKDLNAEDLKKYNLILIGCTG
                     CNSILYYFSDKIPVKLNQEGMHVHDTMIPNPEREGVYIVPNPSNPWSIVLADEFFYST
                     DNFDTHNLNVDYYLRNLKTGDSTKGYFHKFDKNLWEAPVTPGVKLDSPKNNQIEMFEN
                     TFTLKGNATTDFYITYNQ"
     gene            complement(2411..3202)
                     /locus_tag="PK717_01780"
     CDS             complement(2411..3202)
                     /locus_tag="PK717_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46429.1"
                     /translation="MDKDNLRQIINVVATAAMIFVNYLANALPIGGKNTGELAGLYYN
                     DPVRVSIIPSPYAFAIWGLIYLLLAFFVIYQALPSQKGNEISRKIGWWYSVTCLFNIM
                     WIFFWHLLDHVYSIIPMLFLLLSLIMLYQKLETGKAGVSLVDRLAYKAPFSIYLGWIT
                     VATVVNVSVFIVTVPSLHQFFLSIASAEIWSILVLVVATLIGSATAFVKKDYLYPLVL
                     VWALFAIATRNDFSSTISIISIICASLMLIDSIFVVILRVRNKTI"
     gene            complement(3262..4242)
                     /locus_tag="PK717_01785"
     CDS             complement(3262..4242)
                     /locus_tag="PK717_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOR46430.1"
                     /translation="MDKNARDLLDSAVLQKGLAQFGLDPGSARPCEACENFVYDYNDE
                     TIVRFTHSGHRTLEQVLSELDFVSYLSCNGVPCSRPVCGTDGRLGYRIDAGNSYFAVS
                     AFEKAKGVPPSRENTTKEIISRLGQVIGMTHRLAKSYVPSSGIEKRPHLFEDEYYLNP
                     EKYFLEGDTEMLQAFRKNMDRCRLIEASTDNFGLVHTDFHQGNFFVEGGEITVFDFDD
                     SAYFYFADDLAMPLFYHMSGVSENRVQVATEFFASLIEGYKKENILSTDQLGLMGDFF
                     ALRETLLFGLIGRFVDLQNMSPDKKVKLMERRSAILSGRPFLDIDFRQFA"
     gene            4382..4843
                     /locus_tag="PK717_01790"
     CDS             4382..4843
                     /locus_tag="PK717_01790"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOR46431.1"
                     /translation="MEEMDKDIIRDYSGALDEEKQLRFKIEFYGNMKNCWINLAICLS
                     VGFFGIHKFYLGQKNAGFLYFGMGLASIILLMIGKIVALCCSPIIPMLRTGMMGKFFF
                     ECGLLPVILFVILGSLIALVLLILIVIDIFTFKKQTREANTEIAKTISSGL"
     gene            4979..5419
                     /locus_tag="PK717_01795"
     CDS             4979..5419
                     /locus_tag="PK717_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOR46432.1"
                     /translation="MEDITDAADSELINEAIRNIPLDRHDEYKIKYLQQKQNHWITLL
                     MCIFMGPIGAHKFYLQDPWAGYGYMGLFAARMVLFYYINMTRFEYYIHPKPTLEIIMN
                     IITYTLCLLILYDLLTFKKQTKEINKQIARKIVEEMNFSQNGKK"
     gene            5489..8455
                     /locus_tag="PK717_01800"
     CDS             5489..8455
                     /locus_tag="PK717_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46433.1"
                     /translation="MGAGKSFITKIILIIFTFQLCGIQTISQISASDLGAIKDWETNK
                     DEYTRLIPTAVSEDGLVYGVNDDALSKTEYAMMCIGNGKVKWISNEKLDSFGGNWQSI
                     IIKNYIVTMSVINIYVSSDVTFICHTLDGKKMWQKDFKPQKTIPSFEKMGDKLLLYFD
                     EKIIFINVESGDEIKTIDFNSISISQAQYKTNQTLEYEPEYNIIKLLGNKLIIGRKNY
                     LRCFDIDENGSLKEAWSRIFHLEGYFAYLNLYLWKQSIESWILSVEDDKSLDCVSIKD
                     GLKKWAIEVGSLEYARIDYNNNYIIYNLHSSLTVFSFKDQTKLWETKRFDGRFSGFDD
                     KFLYTSQKGLSIYGIENGEKLASVIEQDADMVLIINNGIYMAKKHSFAKYSLCNPCKC
                     KIETFWEKTKNDTINLEVCPLEEKQANFILKNDNPSAIVECSLKSSSDHIILSETAVT
                     LNPENEKTIMVRYCAKKDEPDISTEEITVTTNCNQEIKLVIQTTRKTDCKEKLKKIWE
                     TEGFNFYLFGSNLVDIKSDLKALDKTIGSVNFKTGKQAWSLKLSQIAPENMPIEDVIM
                     HGVNLVLRLKAEGKDDLWVSIDTKNGKLKWKRNGGEPWAEDEYLSNENRVVTYFSLDT
                     GKTLCSSIKIPDYAYITPVFIKNDKYFLKFDCSEDELKETVCYDAKGKILWKTKRFYL
                     DYLGQGKCKNLYLTDINISDDFQKEEAKQHKLVRIDPATGKVLWSFEIIGIPEAISES
                     DTMLYFFDTYGIYCLNNKDGSIIWQEKSHKIILSADILNGFIVYRNSNGDDKAGGIDL
                     YVLDPKNGKALFKKEFNWYVRWSTENNNLFINDLQYDDQGQFVDVFCIDMKKWELIWK
                     ATGRGEARVIGQKTIFISENDLSWFFDGNPLGNAELEECTSGEDRFENDVPKWFGNNI
                     FVKNGSYLQSIDASTGKTKWKLRKTGWPNMQKIIETRKRGAFNILIGELKTPCVIDGI
                     IFNSEGKNLVCYKETD"
     gene            8593..>8982
                     /locus_tag="PK717_01805"
     CDS             8593..>8982
                     /locus_tag="PK717_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46434.1"
                     /translation="MRKIVSILIAIVISMFFVLNPMGAGKIASLSEKYFDTRDRYFDI
                     VAYGYDGPQKIEYKEPSPLFRYYEGAITFFEFEIKDQDGTNIVAPKFDENGNPIKIKQ
                     NDPKIIEKILLCLSPMINAKIGNNSKNF"
BASE COUNT         2953 a         1672 c         1773 g         2584 t
ORIGIN      
        1 ctgattataa gtgatgtaga agtcagttgt tgcattaccc ttgagcgtaa atgtgttttc
       61 aaacatctca atctggttat tttttggaga atccagtttt actccaggtg taacaggggc
      121 ttcccagagg ttcttgtcaa acttgtggaa ataacccttt gtagagtctc ctgttttaag
      181 gttcctcagg taatagtcaa cattcagatt gtgcgtatca aaattgtcgg tagaatagaa
      241 aaattcatct gcaagtacga tactccacgg gtttgaagga ttgggaacga tataaacgcc
      301 ttcgcgctct gggtttggaa tcattgtgtc atgaacatgc atgccttcct ggttcagttt
      361 gacaggtatt ttgtccgaga aatagtatag gatgctgttg caaccagtgc atccaatcag
      421 gatcagatta tattttttaa gatcttctgc attgaggtct ttgtccaaaa ctattggcac
      481 atcaagattg taatactttt ttagcatctt tttgtagaac tgcgccctgg catcggcagc
      541 cttcaccatg agttcatttc cctgccatgt tccttctgca atgatgaaat tgccaaggta
      601 gaagaagttt gaaattgggc ctttagttag agtattgact attgattcgt ctgtaagaat
      661 ataaccttcc gggttgtgga tccttgctcc aacaggttct ttcttggatg aaaggatttt
      721 ttccatatca ggatcattga ggtcccagtc aaagctctca actgttcgat cggtcattat
      781 cacatcaatc ttgaaaggca tcttgaaagg agtttttgat tccagcgtta ttttgtattt
      841 gtaaggattt ttaccagggg tgagaacaaa atcaatatta taagtcaacc ttccagcttg
      901 atttacccac tgctcaaaga accaatccag cttcattcca gaagcctttg acaactcctg
      961 cgagatgtca gatatggtgg caaactttcc agcaaacttg cctgcgtaat ttttgagtcc
     1021 tttgaagaat gatgcttctc cgattacctg gcgcagcatg tggaacacca gtgggccttt
     1081 tttcctgaaa tagatatcca gtttgtcttc ctgggtgatt gaggaaagcg gtttttcatc
     1141 ctttggctgc actttttcaa aatactcgac tcttgccttc ctcagcatct cgatcatctt
     1201 tgattcgcca agtttctggc ctatatagag gatcccagaa tacgttgaaa gcgatgtgtt
     1261 aagaaattcg gcttccggcc caacaccgaa tacagtgcca ccccaccagg aggaggcaac
     1321 tctttctgcc agatattcag gatcggccag atcaaggatt tttttgtctt ttttgtcctt
     1381 ctcttcaacc ttggcaaaag ctgctgttgg aatgaagaga gacgcccttt cagcaataac
     1441 tgttttaaac ttatcaggca tctggatgac catataattt tttggcgcag gggtgccaaa
     1501 aacagactcg tagtatgcca gaagatcgaa aatcgcagat gatatttctt ttccacgatc
     1561 aagcttgtcc ttcgcgccaa agaatgtagt cttggaaata actttctttg gatcttctat
     1621 cttcagtatt tcgtatggac ctgcgatgaa atggtagaag aaggatggat ctctggactg
     1681 gatttttatc tcattgaaag ctttgtcttt gcttatctgt tctgaaatga tatccgaaga
     1741 agcagccttc catccaattg gcagattggc agttatattc attgaaataa catcggaata
     1801 accagcagct atggtaaaga gtgttacagg ataccaccac tggaaaaagt aaatcccatc
     1861 ctgcccaaca taattccatg ccctgcccgg atattctgtt ccagccaatc tggcaacata
     1921 attgattgaa attgtaaacg ttttgtcttt tttgccctgg actggtatta tcagcagatc
     1981 tttttcctgg atgaaatctg acaatccttt tacagatttc actttgaata tcgggtccat
     2041 gtaaaggtaa acatttttga tgtcctggaa ggccttgaat tcaatctcgc aaacaatgtc
     2101 ggtggtgtta tccttcaagt tttgcgacat cgcaatggtg taggcagtaa tttgataact
     2161 tggtttgata gccgcctgaa ctgtgccagg agagattaca gaaaaactag gtgccaatat
     2221 cgatgcacac accattaaaa ccgtcatgat cctgatgaca gacctggatg tcataaaaca
     2281 ccttccttgg ttacattgtt gtgaatgagt atattactgc cagaatacca gtcaacaagc
     2341 cttcaaaatc gtcaaatcaa gcttgaattg gtttctggtt aaattctata taacaacaag
     2401 caatgcatct tcagatcgtc ttgttccgta cccttaatat aacaacaaat attgaatcga
     2461 tgagcatcaa tgatgcacat attatagaaa ttatgctaat ggttgaagaa aaatcgttcc
     2521 tggttgcaat tgcaaaaagc gcccagacca acacaagcgg gtatagataa tccttcttaa
     2581 caaatgctgt cgccgagcca atgagcgtgg ccacaaccag gaccaggatc gaccagattt
     2641 cggcagaagc aatggaaaga aagaactggt ggagtgatgg gactgtgaca ataaaaaccg
     2701 aaacattaac aacggttgcc actgttatcc agccaaggta tatggaaaat ggcgctttgt
     2761 aggccagcct gtccacaagc gaaactccag ctttccctgt ttcgagtttc tgataaagca
     2821 ttatgagtga aagcagcaaa aacagcattg gaatgatgga atacacatgg tcgagcagat
     2881 gccagaaaaa aatccacatt atattaaaca ggcacgttac agaataccac cagccaatct
     2941 tcctggagat ttcatttccc ttctgggacg gaagcgcctg gtagatgaca aaaaaagcaa
     3001 gcaggagata aattagcccc catatcgcga atgcataggg tgaagggatg atggacactc
     3061 tgaccggatc gttgtaataa agaccggcaa gttcaccagt attttttcca ccaatcggga
     3121 gtgcattggc aagataatta acaaaaatca tcgccgctgt ggcaacgaca ttgataatct
     3181 gtcttaaatt gtccttgtcc attttctctc ctccaatttt tggtggaaag attttaccag
     3241 tttttttatg ttttggaaac cttatgcaaa ttgtctgaaa tcaatgtcaa ggaaaggcct
     3301 gccagaaagt attgcgcttc tccgttccat caatttgact tttttatcgg ggctcatgtt
     3361 ttgcaaatcg acaaaccttc caatcaaacc aaaaagaagg gtctccctga gagcaaagaa
     3421 atcacccatg agaccaagct ggtctgtact taaaatgttt tccttcttgt acccctcaat
     3481 cagactggca aagaattctg ttgcaacctg gaccctgttt tcacttactc cggacatgtg
     3541 gtaaaaaagc ggcattgcaa gatcatcggc aaaataaaaa tacgccgaat cgtcaaaatc
     3601 aaaaaccgtt atttcaccac cttcaacaaa aaaattcccc tgatgaaaat cagtgtgcac
     3661 cagcccaaaa ttgtcggttg atgcttcgat cagcctgcat ctgtccatat tcttcctgaa
     3721 ggcctgaagc atctctgtgt cgccctcaag gaaatatttt tctggattca ggtagtattc
     3781 gtcttcaaaa agatgcggtc tcttttcaat ccctgaggac ggaacataag atttagccag
     3841 cctgtgggtc atccctatga cctgcccaag ccttgatatt atttccttcg ttgtgttttc
     3901 cctggaagga ggcacacctt tggctttttc aaaagctgaa acagcaaaat aggagtttcc
     3961 tgcatcaatc ctgtatccca atctgccatc tgttccacaa acaggtctcg aacaaggcac
     4021 tccattgcaa gaaagataag agacaaaatc aagctcggaa agcacctgct caagcgtcct
     4081 gtggcccgag tgggtgaacc tcacaattgt ttcatcattg taatcataaa caaaattttc
     4141 gcaggcctcg caaggtctgg ccgatcctgg atccagacca aattgtgcga ggcccttttg
     4201 cagaaccgca ctatctagga ggtcacgtgc atttttgtcc atgcaatagt atttgacagt
     4261 ttgaagagaa aaaagttttt aacttgccaa acttagccca aatcaacagt ttgacatcta
     4321 cacaaacagt tctataaaat atcggtatct tttcccagaa aataaaaaac ggaggggtgg
     4381 aatggaagaa atggacaaag atatcatcag agactattct ggagcattag atgaagaaaa
     4441 acaattgaga tttaaaatag agttttatgg taatatgaaa aattgctgga tcaacctggc
     4501 aatttgcttg tcagtcggtt tttttggcat ccacaagttc taccttggcc aaaagaatgc
     4561 cggattttta tacttcggaa tgggcctagc atcgataatt cttttaatga ttggcaaaat
     4621 cgtagcatta tgctgcagtc caataatccc gatgctaaga acaggtatga tgggaaagtt
     4681 cttttttgag tgcgggctcc ttcccgtgat tctctttgta atactgggct cactaatcgc
     4741 tttagttctt ctcattctga ttgtaattga tatttttact ttcaaaaaac agacaagaga
     4801 ggctaataca gagatagcca aaacaatttc atcaggcctt taataaaaaa atagtacctt
     4861 tactaatctc gctccaagcg agattagtaa aggtaaaaat gatcgcactt tgtaaaaagt
     4921 aaatccaata aaattggaac tttaatttat tgataaaaac gggagatcct aaagaaacat
     4981 ggaagatatt accgatgcag ctgatagcga acttataaat gaagctatca gaaatatccc
     5041 cctcgataga cacgacgaat acaaaattaa atatctccaa caaaaacaaa accattggat
     5101 aactttgtta atgtgtatat ttatgggtcc aataggggcc cataaattct atcttcaaga
     5161 tccatgggca ggttatggtt atatgggtct ttttgcggca agaatggtct tattctatta
     5221 tatcaacatg accagattcg agtattacat acatccaaaa ccaacactcg aaattataat
     5281 gaatatcatt acttataccc tttgtttatt aattttgtat gacctcttaa catttaaaaa
     5341 acaaacaaag gaaatcaaca agcaaatcgc cagaaaaatc gttgaagaaa tgaatttcag
     5401 ccaaaacggt aaaaaataag gatattgtat aaaaattatt gcaagtacaa tctgtaggtg
     5461 ataagaacat agaatgcgga ggattgatat gggtgcagga aaaagtttta ttaccaaaat
     5521 aattttgatc attttcactt ttcaattgtg tggaattcaa accatcagcc aaatctcggc
     5581 aagtgatctc ggagcaataa aagattggga gacaaataag gatgaataca caagacttat
     5641 tcctactgct gtgtctgaag atggccttgt ctatggggtt aacgatgatg ctttgagcaa
     5701 aactgaatat gcaatgatgt gcattgggaa tgggaaagtc aaatggatca gcaatgaaaa
     5761 attggattca ttcggaggca attggcaatc aatcattata aaaaattata ttgtaactat
     5821 gagtgtaatt aatatttacg tttcctccga tgtgacattt atctgccaca cattggatgg
     5881 caaaaaaatg tggcaaaaag actttaaacc acaaaaaaca atcccatcat ttgaaaaaat
     5941 gggggataaa ttactcttgt attttgatga aaaaattatc tttattaatg tagaatctgg
     6001 cgatgaaatt aaaacaatcg atttcaactc tatttcaata agccaggctc aatacaaaac
     6061 aaaccaaact ctcgaatatg agcctgaata taatattatc aaattattgg gcaacaaact
     6121 tatcatcgga aggaaaaact atcttcgttg ttttgatatt gacgaaaatg gcagcttgaa
     6181 agaagcttgg tcaagaattt tccatctaga gggctacttt gcatacttaa atctgtactt
     6241 gtggaagcaa tcgattgaat catggatttt atcagtagaa gatgataaat cactagattg
     6301 tgtctcgatt aaggatggac tcaaaaaatg ggctatcgag gttggatctt tggagtatgc
     6361 ccgaattgat tacaataaca attatattat atacaacctt catagctctc taaccgtttt
     6421 ctcatttaag gatcaaacga aattgtggga aacaaagaga ttcgatgggc gcttttctgg
     6481 ttttgatgac aaatttttat atacctccca aaaaggtttg tctatttatg gcatagaaaa
     6541 tggcgaaaaa ttagcctcag tgatcgagca agatgcagat atggtgttaa ttataaataa
     6601 cggtatttac atggcgaaaa aacattcttt tgcaaaatac tctctttgca atccatgtaa
     6661 atgcaagatc gaaacatttt gggaaaaaac caaaaacgac acaatcaatc ttgaggtgtg
     6721 cccccttgaa gaaaaacagg caaatttcat tctaaaaaat gataatccat cggcaattgt
     6781 tgagtgttca ttaaaatcct cctccgatca tatcatattg agcgaaacag cagtaacgct
     6841 gaatcctgaa aatgaaaaaa caataatggt cagatattgt gcaaaaaaag acgaaccgga
     6901 catatccaca gaggaaataa cagtgaccac aaattgtaat caagaaatca aactggtcat
     6961 ccaaacaaca agaaaaaccg actgcaaaga aaaattgaag aaaatatggg aaacggaggg
     7021 ttttaatttt tatttgtttg ggagcaatct cgtggatatc aaatctgacc ttaaagctct
     7081 agataaaacc attgggtctg tgaacttcaa aactggcaaa caagcgtggt ccttgaaact
     7141 aagccaaata gccccagaaa acatgccaat agaggatgta ataatgcatg gagtcaatct
     7201 ggttctccgc ttgaaagccg aaggcaagga tgatttatgg gtatctattg atacaaaaaa
     7261 tggcaaactc aaatggaaaa ggaacggcgg ggagccatgg gcagaagatg aatatctgtc
     7321 caatgaaaat cgcgttgtaa cctatttcag tctggataca ggaaaaacat tgtgttcttc
     7381 aataaaaatc cccgattatg cttatataac tcctgtattt ataaagaatg acaaatactt
     7441 tttaaaattt gattgcagtg aagacgaatt gaaagaaaca gtttgttacg acgccaaggg
     7501 gaagattctc tggaaaacaa aaagatttta tctcgactat ctaggacaag ggaaatgcaa
     7561 aaatttatat ttaaccgata taaacatcag cgatgatttt caaaaagaag aagcgaaaca
     7621 gcataagctt gtaagaattg atccagccac aggaaaagtt ttgtggtcat tcgaaattat
     7681 cggcatacct gaggcaataa gcgaaagcga caccatgctg tatttttttg atacatatgg
     7741 tatttattgc ctaaacaaca aggatggatc gattatctgg caagagaaat cccataaaat
     7801 aatattgagt gcagatattc taaatggttt catagtatac cggaatagca atggtgacga
     7861 taaagccggc ggaattgatc tttatgtttt agatccgaaa aatgggaaag ctctctttaa
     7921 aaaagaattc aattggtacg tgagatggag tactgaaaac aataatcttt ttatcaatga
     7981 tctgcaatat gatgaccaag gtcaattcgt cgatgtgttt tgcattgaca tgaaaaaatg
     8041 ggaacttatc tggaaagcaa caggcagagg cgaagcaaga gtaatcgggc agaaaacaat
     8101 ttttataagt gaaaacgatc tttcatggtt ttttgatggc aacccccttg gcaatgctga
     8161 acttgaggaa tgcacatctg gggaagatag atttgaaaat gatgttccaa aatggtttgg
     8221 caacaacatt tttgtaaaaa atggcagtta cctacaatca atagatgcat caactggcaa
     8281 aacaaaatgg aaattgcgca aaacaggctg gcccaatatg caaaaaatta tagaaacaag
     8341 gaaaagaggg gcttttaaca ttctcattgg ggagctcaaa acaccatgtg tcattgatgg
     8401 aattatcttc aattctgagg gtaaaaatct ggtctgttac aaagagacag attgatccac
     8461 tgattaaaaa gctattattt agcattagaa ggttttgtta tatatgactg tgaggatatt
     8521 tttgatattg caatagcaag gtttttctta taataaacat gtctgaaaaa actgggaact
     8581 agaggaggga cgatgagaaa aattgtaagt attctgattg ccattgtaat ttcaatgttt
     8641 tttgtgctta atccaatggg tgccggcaaa attgccagtt tgagtgaaaa atatttcgat
     8701 actcgcgaca ggtattttga tattgtggca tacggatatg atgggccaca aaaaatcgag
     8761 tacaaggagc catcaccact tttcaggtat tatgaaggag ccatcacttt ttttgaattc
     8821 gagataaaag atcaagatgg gacaaatatt gtagccccaa aattcgatga aaatggcaac
     8881 cctatcaaaa tcaaacaaaa tgatcccaag attattgaga aaatcctact ctgtttgtct
     8941 cccatgataa atgcaaagat tggcaacaat agcaaaaact tt
//
LOCUS       DAOSRI010000034         8711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7695_length_8711_cov_4.403189, whole genome shotgun sequence.
ACCESSION   DAOSRI010000034 DAOSRI010000000
VERSION     DAOSRI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7695_length_8711_cov_4.403189"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK717_01810"
     CDS             complement(<1..456)
                     /locus_tag="PK717_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOR46435.1"
                     /translation="MSNGNNQKNTKMQEVQVKDFLMEFIPELFKNMGEDAHVRVSKEI
                     DTMFVSVELDDPAVYIGKSGECLASLQFIINMILQRKEWNAVRITLDIGGYKRRQIDI
                     LKNIAQNAALKVRRFGRPVELQPMSAFARRIIHTTLKDNPSVFTHSIDEE"
     gene            complement(453..1979)
                     /locus_tag="PK717_01815"
     CDS             complement(453..1979)
                     /locus_tag="PK717_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOR46436.1"
                     /translation="MKKIAAILAITLLTIWPVAGLVSGAQLSVKFSTSVEALAGNSSI
                     VPVEVTNTGSEPIENAFVTIASFDGKPLGLESGARLVQGSQEKIGIQRIDAGSTEKIK
                     FQIEFLSSLKSGIKKITFSLESNGQTATGESEINVNENLNPPSTKKIIVSSDAESYPN
                     SNFTYTVKVENPKSDSDGVMDMVDLKVELLSINGKQLKEFTAEEAKKDSFFRPIFKSS
                     LVVSPDNPERKLAPGDSFEAKFMLATGPDIELGSYKAKIKITWQPADTKLPMQTNEIE
                     GNIEVKQVTWYTFAVRWVIDGLAKTVGFGNYAITIIIFALLVKLCFWPLSNAQFKNME
                     KMAKIQPLLNALKQKYPRKEDAQKLQEETMAIYKENNVNFLSGCLPLLIQLPIVMALY
                     SGISGFAPLNYANFIWLPSLGLADPFYIMPILLAASTWLQQKVSTMPGQDQQSASFQI
                     MFPAMMFLFGINFPSGISLYWFMFNLASIIHQMVYNKGAFGKFIPTGIIKPVSKGIKK
                     "
     gene            complement(1992..2156)
                     /gene="yidD"
                     /locus_tag="PK717_01820"
     CDS             complement(1992..2156)
                     /gene="yidD"
                     /locus_tag="PK717_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011363782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOR46437.1"
                     /translation="MSPIIPSRCIYTPTCSKYAYDAILKYGPIKGGFLAIKRILRCRP
                     GMPGGYDPVP"
     gene            complement(2084..2533)
                     /locus_tag="PK717_01825"
     CDS             complement(2084..2533)
                     /locus_tag="PK717_01825"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOR46438.1"
                     /translation="MKLPHRLTAGQIHNLFKSGSSVYSKALRIVYLEGLESGGAVSYI
                     SPKSLGKAHTRNRARRLLREAFVLSETCVDKNIGIAIIARTSVLGMDPTQLSSEISKL
                     ISQMHSKKHYQSHSCSPYQSTGRSSHQSSLHAASTPQHAQNMPMTQF"
     gene            complement(2530..2667)
                     /gene="rpmH"
                     /locus_tag="PK717_01830"
     CDS             complement(2530..2667)
                     /gene="rpmH"
                     /locus_tag="PK717_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR46439.1"
                     /translation="MRTHMRPHNRKRLRVSGFRTRMRTRGGRAVLARRRARGRWRIAI
                     S"
     gene            2809..5451
                     /gene="ppdK"
                     /locus_tag="PK717_01835"
     CDS             2809..5451
                     /gene="ppdK"
                     /locus_tag="PK717_01835"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOR46440.1"
                     /translation="MERLIYHFEDAIAVSEKDQKAVLGGKGAGLAKMTSIGMPVPPGF
                     TIVTFGCEYYYGPWQEKLPEDFKKDILEHIKGVESKTGKKFGNPGNPLLVSVRSGAAV
                     SMPGMMDTILNLGLNDETAAGFAKLTNNERFAFDCYRRLMQMFGNVVLGINHDDFEEV
                     LDHVKESAGAKTDQDLKADDLKKVIEGYKQVVLKATGKPFPQDPNEQLFAAIIAVFKS
                     WNNPRAHAYRQLYDIRGLRGTAVNVQTMVFGNMGDDSGTGVCFTRNPSTGDSHYFGEY
                     LTNAQGEDVVAGIRTPSPIAKLEQEMPICYKQLTEAFERLEKNYRDMQDVEFTIERGK
                     LYLLQTRNGKRAAQAAVKIAVDLVSEGIIDKEEALMRVNPQELDQLLHKRLDPKSKAS
                     AKAIAKGLPASPGAAVGQAVFTAEDAIEWSEQKKKVILVRAETSPEDVMGMAKSQGIL
                     TATGGMTSHAAVVGRGMGKCCVVGCGAITVSAEGKFFEVGGKKYKEGDWITLDGSTGE
                     VYGEKLETKDPEFTSDFTQFMEWADKTRRLEVRTNADTPFDAKVARNFGAHGIGLCRT
                     EHMFFAPDRIKAMREMILADDLGGRKKALEKLLPYQREDFVGILEAMRGHHVTIRTLD
                     PPLHEFLPHQDEEIKELAADMGISEEKLRNKIESLHEVNPMLGHRGCRLGIVYPEITV
                     MQARAILEAACQLKQQNVEVYPEIMIPLVGDVNELSNQKKLVLEAAEEVFKTMGCRVD
                     FQVGTMIEVPRGALTADDVAKEAEFFSFGTNDLTQMTFGFSRDDVGKFIRDYIDKGIL
                     EKDPFQVLDQRGVGQLVRMAVEKCRKTRPGLHCGICGEHGGEPSSVEFCHTVGLDYVS
                     CSPYRVPIARLAAAQAVIREKRSK"
     gene            5456..7150
                     /gene="dnaG"
                     /locus_tag="PK717_01840"
     CDS             5456..7150
                     /gene="dnaG"
                     /locus_tag="PK717_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR46441.1"
                     /translation="MDLDRLVEEISNKLDIVSVVGRYVKLKKAGRNFLGLCPFHAERT
                     PSFTVSPDKQMFYCFGCRTGGNIVTFVSKIEGLEFREALDKLATEAGIDVPEFKRGPD
                     RSPDYELMSATQEFFRENLKHSPLPQDYLQRRGLDQCIISKFQIGFAPTDGKLLPKYL
                     QSKGFDVERAKVLGVLSREELHSYMRARVTFPIVDNRGRFIAFGGRVLDNSEPKYLNS
                     PATPIFEKGKNLFALNIARSHIAKLGRAIVVEGYMDAISMHKAGFPETVASLGTAFTV
                     DQAFMLRKLGADVFLFFDGDSAGEKAAFSAIRICFQAGLVFKIVRQSVGKDPDDLARI
                     GTDAVNSTLAEAKDPVDFAINYLESQEDDPASPQVRVRIAKQTLELLESSQDLLLREQ
                     YTNYVAKKFGITPSQKTIGNTENNKNKKLNILASRLLYENRIIRALLSDEQARNLLLP
                     CLFADYFVDDSCKKAFCLMLENKVTFGKIAWSYVLENNEELSSIFSRFAIMDEEQDIQ
                     GLIIDVKRDVAAKKQRMIRKEKADSVSLDDLAELARLQKVRHKVQEEQNDKEERQG"
     gene            7461..8513
                     /gene="rpoD"
                     /locus_tag="PK717_01845"
     CDS             7461..8513
                     /gene="rpoD"
                     /locus_tag="PK717_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOR46442.1"
                     /translation="MPELVDLIRAHKDSSVVSYQEIMDLMGQMNLTADQIDEVYNIFT
                     ELGIRFGDEDKLGEVHPDAADESGPEVKISEEGEVSDPIRMYLREIGKIPLLTLQGEI
                     ALAKRVEIGNQDAKKKLIEANLRLVVSIAKKYTGHCLSFLDLVQEGNIGLIRAVEKFD
                     YRKGFRFSTYASWWIRQAITRALADQSRVIRVPVHMVESINKLTKVSRELYQDLGREP
                     TYRELAEKMGTTPRKIREYFKISQEPLSLETPIGEEKDNRLGDFVEDKKVSSPEEQIV
                     NKYFKEQMHSILSILSPREREIIKLRFGLDSEYPHTLEEVGQIFKVTRERIRQIEAKA
                     IRKLRQSVRFKDQRIF"
     gene            8528..>8711
                     /locus_tag="PK717_01850"
     CDS             8528..>8711
                     /locus_tag="PK717_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR46443.1"
                     /translation="MKLNERQLEAVNTTEGAIAVFACAGSGKTRVITNRIAHLIKDLG
                     VAPSSILACTFTNKAAG"
BASE COUNT         2462 a         1897 c         2179 g         2173 t
ORIGIN      
        1 ctcttcatca attgaatgtg tgaataccga gggattgtct ttcagggttg tgtggatgat
       61 gcgccttgca aacgctgaca ttggctggag ttctaccggt cttccgaaac gcctgacttt
      121 gagggcggca ttctgggcaa tgtttttcag gatgtcaatc tggcgtcttt tgtatccgcc
      181 aatatcaagg gtgattctga ctgcgttcca ctccttgcgc tggaggatca tattgatgat
      241 gaactggagg gaagcgaggc actccccgga ttttccgatg tacactgcag ggtcgtccag
      301 ttcgacggaa acgaacatgg tgtctatttc ttttgatacc ctaacatgcg catcttcccc
      361 catatttttg aagagttcgg ggatgaattc cataaggaag tcttttactt ggacttcctg
      421 catctttgtg tttttctggt tattgccatt gctcatttct ttattccctt tgaaactggt
      481 ttaatgattc ctgttggaat gaattttcca aaggctcctt tattatagac catttgatgg
      541 attatagagg caaggttgaa catgaaccag taaagagaga tgcctgatgg gaaattgatg
      601 ccgaagagga acatcattgc cgggaacatg atctggaatg acgcactctg ctgatcctgt
      661 cctggcatcg tagaaacttt ttgctggagc catgtagaag ccgcaagaag gattggcatg
      721 atgtagaatg gatctgcaag accaagcgaa ggaagccaga taaaattggc atagttgagt
      781 ggagcaaatc cgcttattcc ggaatagagg gccatgacga ttgggagctg gattagaagt
      841 gggaggcacc cggaaaggaa attgacatta ttttccttgt agatggccat cgtctcttcc
      901 tggagcttct gtgcatcctc ttttcttgga tatttctgtt tgagtgcatt cagaagtggc
      961 tgtatcttgg ccatcttttc catgttttta aattgggcat ttgagagcgg ccagaagcac
     1021 agcttaacaa gcaatgcgaa gatgattatt gtaattgcat aattgccaaa cccaaccgtt
     1081 tttgcaagac catcaatgac ccacctgact gcaaatgtgt accaggtgac ctgcttgact
     1141 tctatgtttc cttcgatctc atttgtctgc attggcagtt ttgtatctgc cggttgccag
     1201 gttatcttta tttttgcctt gtatgaacca agctcaatgt ctggtcctgt tgcgagcatg
     1261 aattttgctt cgaaagaatc accaggggca agctttcttt ccgggttgtc tggtgaaacc
     1321 acgagcgagc ttttgaaaat tggcctgaaa aaactatctt ttttggcttc ttccgctgtg
     1381 aattccttga gttgttttcc atttatcgac agaagctcga ccttcaggtc aaccatgtcc
     1441 attacgccgt cactgtcact ttttggattt tcaaccttga ccgtataagt gaaattgctg
     1501 tttggatagc tctcggcgtc tgagcttaca ataatttttt tggtgctcgg cggattgagg
     1561 ttttcgttga cattgatttc cgattcgcca gtggctgttt ggccatttga ctcgagagag
     1621 aatgtaattt ttttgatacc agattttagg gatgacaaaa attcgatctg aaattttatt
     1681 ttctcggtgc tgcctgcatc aattctctgg atgcctattt tttcctgcga accttgtact
     1741 aacctggcac ccgactccag acctagcggt ttgccatcaa aagaagctat tgtaacaaat
     1801 gcgttttcaa ttggttcgct gccggtgttt gttacttcta ctggaacaat actgctgttc
     1861 cctgccaatg cctcaacaga cgtgctgaat ttgactgaga gttgagctcc tgataccaaa
     1921 ccggcaaccg gccagatggt cagaagtgtt atcgccaata ttgctgctat ctttttcatt
     1981 aatactcctt tttaaggaac cggatcatat ccgcctggca ttcctggcct gcaccttagg
     2041 attcttttga tggctaggaa gccacccttt attggtccat attttaaaat tgcgtcatag
     2101 gcatattttg agcatgttgg ggtgtagatg cagcgtgaag ggatgattgg tgagatgagc
     2161 gtccggtaga ctgatatgga gaacaggagt gggactgata atgcttcttt gaatgcatct
     2221 gtgatatcag ttttgatatt tcggaagata gttgagtcgg gtccatcccc agtactgacg
     2281 tccttgcaat tattgctatg ccaatgtttt tgtcgacaca tgtttcagat aatacaaaag
     2341 cctcccgtag aagtctcctt gccctgtttc ttgtgtgtgc tttcccaaga ctttttggtg
     2401 agatgtaaga tactgcgcca ccagattcaa ggccctccag ataaactatc ctgagggcct
     2461 ttgagtacac cgatgaacct gatttaaaaa gattgtggat ctgccccgcc gttaagcgat
     2521 gggggagttt catgaaatgg caattctcca acgacccctg gctctcctgc gagccaaaac
     2581 ggcccttcca cctctcgttc tcatcctagt tctaaagcca gagacgcgaa gacgcttgcg
     2641 attgtgtggt ctcatatgtg tgcgcataat aaatacctcc aatacaatgt cgttagattc
     2701 taaccatcca gtgaccataa tgtcaataaa gaaaggattt ggctttgttg acataaatca
     2761 ctttctgttg gaaaatcaaa gggttaaaaa atacgactgg agagtgcaat ggagaggcta
     2821 atttatcact ttgaagatgc aattgcagtg tctgaaaaag accagaaggc agtccttgga
     2881 ggaaaaggcg ccggactggc caaaatgacg tcaattggga tgcctgtacc cccaggtttc
     2941 acaattgtca catttggttg tgaatattat tatggaccat ggcaggaaaa actccctgag
     3001 gatttcaaaa aagatatcct cgaacacata aagggcgtcg aatcaaagac tggcaagaag
     3061 tttggcaatc caggaaaccc gctactggtt tcagttcgtt ccggtgccgc cgtttcaatg
     3121 cctggcatga tggataccat cctgaacctt ggactcaacg atgagactgc tgcaggattt
     3181 gcaaagctta caaacaacga aaggtttgct tttgactgct atcgcaggct catgcagatg
     3241 tttggaaacg ttgtcctcgg gatcaatcac gacgattttg aagaagtcct tgaccacgtt
     3301 aaggaatctg ctggcgcaaa gaccgatcag gatctcaaag cggatgatct caagaaagtc
     3361 atcgagggct acaagcaggt tgtcctcaag gcaacaggaa agccattccc gcaggaccca
     3421 aacgaacagc tctttgctgc aattatcgca gtcttcaaat catggaacaa tccaagggct
     3481 catgcctaca gacagcttta tgacatcagg ggtctcaggg gaactgcagt caatgtccag
     3541 accatggttt ttggaaacat gggcgatgat tcgggcactg gcgtatgttt tacaaggaac
     3601 ccttcaacgg gagacagcca ctattttggc gaatacctta caaatgccca gggagaggac
     3661 gtcgttgcag gcatacgcac cccgtcacca atagcaaagc tcgagcagga aatgccaata
     3721 tgctacaagc aactcacaga agcattcgag agactcgaga aaaactacag ggacatgcag
     3781 gacgtagagt tcaccattga aagaggaaaa ctgtatctcc ttcaaacccg taacgggaaa
     3841 agggccgccc aggccgccgt caagatagca gttgacctgg tttcggaagg aatcatcgac
     3901 aaggaagagg ccctcatgag ggtcaatccg caagaacttg accagcttct ccacaagagg
     3961 cttgatccaa aatccaaggc gtcggcaaaa gccatcgcaa aaggccttcc ggcatcacca
     4021 ggtgctgcag ttgggcaagc cgtctttact gcagaggacg caatagagtg gtccgagcag
     4081 aaaaagaagg tcatcctggt cagggctgaa acctctccgg aagacgtaat gggcatggca
     4141 aagagccagg gaatcctcac agcaacaggc ggaatgacat cacatgccgc agttgttggc
     4201 agaggaatgg gtaaatgttg tgttgttggc tgcggtgcaa taactgtcag cgcagaaggt
     4261 aaattctttg aagttggtgg caaaaaatat aaagagggtg actggataac gctggatgga
     4321 agcactggcg aagtgtatgg cgaaaagctt gaaaccaagg accctgaatt tacaagcgat
     4381 ttcacccagt ttatggaatg ggcagataaa actcgcagac tggaagtccg caccaatgca
     4441 gacaccccgt ttgatgcaaa agtggcaaga aactttggcg cccatggcat cgggctctgc
     4501 agaacagaac acatgttctt tgccccagac cgcatcaagg caatgaggga gatgatcctt
     4561 gctgatgacc ttggtggtcg caaaaaagca ctcgaaaagc tcctaccgta ccagagagaa
     4621 gattttgttg gcatccttga ggcaatgaga ggccatcatg taaccatcag gacgctcgat
     4681 ccgccgctcc acgagttcct ccctcaccag gatgaagaaa taaaagagct tgcggccgat
     4741 atgggtattt ctgaagaaaa gctccgcaac aagatcgagt cccttcacga agtaaacccg
     4801 atgcttggcc acaggggttg caggctcggg attgtatatc cggaaataac agtaatgcag
     4861 gcaagggcca ttcttgaggc cgcatgccag ctcaaacagc aaaatgttga agtctatcct
     4921 gaaatcatga tcccgctcgt tggtgatgtc aatgagcttt caaaccagaa aaagcttgtt
     4981 cttgaagcag ccgaagaagt cttcaagaca atgggttgca gggttgattt ccaggttggc
     5041 acaatgatag aagtgccgag gggcgccctc actgctgatg atgttgccaa ggaagcagaa
     5101 ttcttcagct ttggaacaaa cgacctcacc cagatgacct tcggtttctc aagggatgac
     5161 gttggcaagt tcatcaggga ctacattgat aagggcattc ttgagaaaga cccattccag
     5221 gttcttgacc agcgtggtgt tggccagctt gtcagaatgg cggtcgagaa gtgccgcaag
     5281 accagaccgg gccttcactg tggaatatgt ggagagcatg gcggagaacc atcctcagtg
     5341 gagttctgtc acacagtcgg actagactat gtctcctgct cgccatacag ggtgccgata
     5401 gcgcgccttg cagcagccca ggccgtgatc agggagaaga ggagcaaata agcatatgga
     5461 cctcgatcgg ctggtcgagg agatatcaaa caaactcgac attgtaagtg ttgtcgggcg
     5521 ctatgtaaaa cttaaaaagg ccgggagaaa ttttctcggc ctttgtcctt ttcatgcgga
     5581 gcgcacgccg agttttacgg tctccccaga caagcaaatg ttctattgtt ttgggtgccg
     5641 gaccggcggc aatattgtca cctttgtctc caagattgaa gggctggagt tcagggaggc
     5701 actagataag ctggccactg aagcagggat agatgtcccg gaattcaaga gaggccctga
     5761 ccgttctcca gattatgagt tgatgtctgc aacccaggaa tttttcaggg aaaatctcaa
     5821 gcatagtcct ctcccccagg actacctgca gagaaggggt ttggaccagt gcatcatatc
     5881 caaattccag ataggttttg caccaactga cggaaagctc ctcccaaagt atctgcaatc
     5941 aaaaggtttt gatgtcgaaa gggcaaaagt gcttggcgtt ctttcaagag aagaactaca
     6001 ttcatacatg agggcaagag tgaccttccc gatagttgac aacagaggca ggttcatcgc
     6061 ttttggtggc agggtgcttg acaactccga accaaaatac ctgaattcgc cggcaacacc
     6121 gatatttgaa aaaggaaaaa acctttttgc actgaatatc gcaaggtcgc acattgcaaa
     6181 acttggcagg gcaattgttg ttgaaggcta catggacgca atctcgatgc acaaagcggg
     6241 ttttcccgag accgttgcat cattggggac agcatttaca gtcgatcaag ctttcatgct
     6301 gagaaagctt ggtgccgatg tgtttctctt cttcgatggt gactctgcgg gagaaaaagc
     6361 agcgttctcg gcaatcagga tttgctttca ggcagggctt gtattcaaga ttgtgaggca
     6421 atcggttggc aaggatccgg atgatctcgc aagaatagga actgatgctg tcaattccac
     6481 actggccgaa gcgaaagacc ctgttgattt tgcaatcaat tatctcgaat ctcaggaaga
     6541 tgatccagcc tcgccacagg tcagggtaag gatagcaaag cagacccttg aattgttgga
     6601 aagctcccaa gatctcctat tacgtgagca gtacaccaat tatgtggcga agaaatttgg
     6661 gatcacccct tcacaaaaga caattgggaa taccgaaaat aataagaata aaaagttaaa
     6721 cattttagcc tcgagattgt tgtatgagaa caggataata agggcactcc tttctgatga
     6781 gcaggcaaga aatttgttac tgccttgcct ttttgctgat tactttgttg atgattcttg
     6841 taaaaaagcg ttttgtttga tgttggaaaa taaggtaact tttggtaaaa ttgcttggtc
     6901 atacgtactg gaaaacaatg aagaattatc ctccattttt tccaggtttg ccattatgga
     6961 tgaggaacag gacatccagg gattaattat tgatgtaaag agggacgtgg ctgcaaaaaa
     7021 acagaggatg ataagaaagg aaaaagcaga cagtgtttct ctggatgacc ttgctgaatt
     7081 ggcaaggttg cagaaggtgc gacacaaggt tcaagaggaa caaaatgaca aagaagaacg
     7141 acaaggttga ggccactggc aagaccgaaa aaaaggccac cacgcagcca aaaacccaaa
     7201 aggcaaaggc tggggcaaaa actgtagaaa aagaaaaagc cgttgccaaa caagctgtac
     7261 cagagaagac caaaaaggtc aaggaacaac cgaaggcaga gcctaaaaat aacacccctc
     7321 caaaaaaggt aaagtcggtt aaatctgcac aggaacagcc tgccgcacaa aagtcctctg
     7381 atgcggcgtt tcttgaacac ctgaagaaaa aattatatat caggccaaac gacacttcga
     7441 taagagcaga actcatcgaa atgccagaat tggtcgattt gataagggca cacaaggatt
     7501 ccagtgttgt aagctatcag gaaataatgg acctgatggg ccagatgaac ctcacggccg
     7561 accagatcga tgaggtttac aacatattca ccgagcttgg aataaggttt ggcgatgaag
     7621 acaagcttgg ggaagtacac cccgacgctg cagatgaaag tggccctgaa gtaaagattt
     7681 cggaggaggg tgaagtctcc gacccgatca gaatgtacct cagagagatt ggtaaaatac
     7741 cactccttac attgcagggt gaaatagcac ttgccaaaag agtcgaaata ggcaatcagg
     7801 atgcaaagaa aaaactgatc gaggcaaacc tcaggctcgt tgtctcgatt gccaagaaat
     7861 acaccggcca ttgtctttcg ttccttgatc ttgttcagga agggaacatt ggtcttataa
     7921 gggctgtcga gaagtttgat tacagaaagg gtttcaggtt ctcgacctat gcttcgtggt
     7981 ggataaggca ggccataacg agggcgcttg ccgatcaatc aagagtcatc agggtaccag
     8041 tccacatggt ggaatccatc aacaaattga ccaaggtctc aagagagctt taccaggacc
     8101 ttggcaggga gccgacctac cgcgagcttg ccgaaaaaat gggcaccact ccaagaaaaa
     8161 tcagggaata cttcaaaata agccaggaac cactttcgct tgaaacgccg attggcgaag
     8221 aaaaggacaa caggcttggc gattttgttg aagacaaaaa ggtttcctcc cctgaagagc
     8281 agatagtaaa caagtatttc aaggaacaga tgcacagcat cctgtcgata ctttcgccga
     8341 gggagaggga gataataaag ctccgttttg gtctggacag tgaatatccg cacacgctgg
     8401 aggaggtcgg ccagatattc aaggtcacca gggagaggat ccgccagatt gaggccaagg
     8461 caataagaaa gcttaggcag tcggtaaggt tcaaggacca gcggatcttc taggccagtc
     8521 aaatccaatg aagctcaatg aaagacagct tgaggctgtc aatacaactg agggcgcaat
     8581 tgctgttttc gcgtgcgccg gctccggaaa gacaagggta attaccaata gaatagcgca
     8641 cctcataaaa gatcttggtg tcgctccatc gtcaattctt gcctgcactt ttacgaacaa
     8701 agcggccgga g
//
LOCUS       DAOSRI010000035         8584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7836_length_8584_cov_4.515301, whole genome shotgun sequence.
ACCESSION   DAOSRI010000035 DAOSRI010000000
VERSION     DAOSRI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7836_length_8584_cov_4.515301"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..400
                     /locus_tag="PK717_01855"
     CDS             116..400
                     /locus_tag="PK717_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR46444.1"
                     /translation="MKNGDGLTSIPGVGKSIAGDLIELGINRVSDLKNRDPQELYDLL
                     CTKKGHQDRCLLYVFRCAIYFASNTNHKPELLKWWNWKDNTEPKRRREAK"
     gene            397..750
                     /locus_tag="PK717_01860"
     CDS             397..750
                     /locus_tag="PK717_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR46445.1"
                     /translation="MKDCFIYIEWYVTNIERAVNFYKGMFNWKFEKSTDDYMMFEMSN
                     GESGGIMEVDKVKPGNSPVFYIHADRIENYLEKAPGLGGSVSVGKTELSGIGFYAFLA
                     DPDGNKIGLYEDLKQ"
     gene            855..1346
                     /locus_tag="PK717_01865"
     CDS             855..1346
                     /locus_tag="PK717_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOR46446.1"
                     /translation="MGKKILVCYATRCGSTGEVAERIASVIKSDGHTVDVSQVKRNID
                     IDDYDAVVVGSAIRIGKCLGEAIEFVKQRKPDLQKKKLFYFGVCLMITMPDKLHEVQT
                     YLDSMIAECTPVAHEIFAGKVDFSKLSFPIRLLMKQMKTPEGDFVEWQKVESWAKMIS
                     KSI"
     gene            1880..4468
                     /locus_tag="PK717_01870"
     CDS             1880..4468
                     /locus_tag="PK717_01870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOR46447.1"
                     /translation="MSYIDITKLRGLSQEEAAERLATDGPNELPSSKRRSIFVIIFEV
                     IKEPMFLLLVSCGTIYLLLGDRQEAFMLLFFVFVVMGITIFQEKKTERALEALRDLSS
                     PRALVIRNGAQQRIAGRDVVRGDFLILQEGDRVPADCILISCINLTVDESLLTGESVP
                     VRKTNCDNEDELMGKPGGDDQPYIFSGTLVVSGQGVAITKSTGSNTEIGKIGKALQKL
                     DKNDDTRLQHEISRLVRNVAFFGLALCIVVFAMYGFTKTDWLGGLLAGLTLAMAMLPE
                     EFPVVLTIFMALGAWRMSKKDVLTRKMPAIETLGSATVLCVDKTGTLTQNKMTVVELM
                     TGGEIIGLKGNEKELPEDFHSLVEFAVLASEKNPYEPMDRAIRELGAKHLGGTEHLHD
                     DWEFIQEYPLSKELFAISQVWKSTKGGGYVIAAKGAPEAIADLCHLDKQAVEDINSQV
                     FKMASEGLRVLGVARAYFMEPGGLPKAQHGFDFEFLGLVGFFDPVRESVPQSIKECYE
                     AGIRVVMITGDYPVTACKIARSIGLENSGEYITGHELDEMSDEALNERIGKVNVFARV
                     VPEQKLKLVQALKAKGEIVAMTGDGVNDAPALKAADIGVAMGGRGTDVAREASSLVLL
                     KDDFSSIVDSVRMGRRIYENIQKALAYIVAIHVPIAGISLLPVLLKWPTLFTPMHIVF
                     MELVIDPACSIVFEAEQGEKNSMKRPPRNPNAPLFGAGVFMLSALQGVGVLLVILLVN
                     FVALRMGRGEFEARTLTFTTLVVSNLALILVNRSWNKAAFEIVKTKNQAFWWVFFGAI
                     VFLGLVLYVQFLQNLFKFTTLHASDILICLCAGLLSVVWFEVFKKLRTRKRRLDARDS
                     KTGTVQ"
     gene            4434..4985
                     /locus_tag="PK717_01875"
     CDS             4434..4985
                     /locus_tag="PK717_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46448.1"
                     /translation="MPEIVKLEQFSEEDLNSVLVGYTSTRKYTIEKTETKNQITFRLD
                     LVPLERVYVKKWFLTNEVDHYRRIVPTGYSIGAYVEGKIVGMAIAENLDWNRSLFIWE
                     FHVKEGHRRQRIGTRMMMELEKEAKQANLRIMVCEAQNTNADAIDFYKHCGFSVEGID
                     LHYYDDLDSRNEFAIYMKKIIGE"
     gene            5109..5459
                     /locus_tag="PK717_01880"
     CDS             5109..5459
                     /locus_tag="PK717_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR46449.1"
                     /translation="MEENFLGEFELSLKVNDLVRSMDFYHKLGFERVDGEPAKGWAVL
                     RCGDLRLGLYQGHLETNLMSFLGGNVKSIAQDLKSKKIEMFSDATQEKDGSMGAKLVD
                     PDGNIIYFNSFTDE"
     gene            5471..5980
                     /locus_tag="PK717_01885"
     CDS             5471..5980
                     /locus_tag="PK717_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR46450.1"
                     /translation="MLNFIEKCRIYGASRVEISKIAKSPADWEKFWREPEFGFPFKWG
                     SCWKQCCEYFVSDFEAEVGFFTDVLGFVCNALGQDYAMFTSPARDFFIAFYKAESDRA
                     TAPGAIKIAFMIEEIDQTVRKLKDRGVEFIDDPQNVAEDSPLVKASFATPNMIECTLW
                     GFLEDKDVC"
     gene            5970..6533
                     /locus_tag="PK717_01890"
     CDS             5970..6533
                     /locus_tag="PK717_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46451.1"
                     /translation="MSAENNDMPMHYVTTRGEAREIAKGKKHYWVSDCGCRKGNESGC
                     KRSPVDVCLCWAGPYGSTEENVRELNEEEIEKLFNLAEEKHLVTRPFRKWNSETKSVI
                     DETDGICFCCDCCCAYFTEPGEACDKGQSIEKTDRDTCTDCGACVDVCYFKARKMEDG
                     KLEIASDNCYGCGLCADVCSCITMAKR"
     gene            6808..7245
                     /locus_tag="PK717_01895"
     CDS             6808..7245
                     /locus_tag="PK717_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46452.1"
                     /translation="MKEIITTNRRVTNPILSVIFGRTAFHNKTKGNAFLVLWDEKEAK
                     RLQGELSMNGWNVRLETKDSFRAYSNIKSTQPDVVVIDSRDNERSFNELAGALRSIKA
                     TRQMPIVAMNCNNEAKSHFADAHSTSEGILETISRFQRQNLNQ"
     gene            7328..>8584
                     /locus_tag="PK717_01900"
     CDS             7328..>8584
                     /locus_tag="PK717_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR46453.1"
                     /translation="MANFVLPIGKKTANNYQMPPKALERLTQAATLESLDLLDLLDSN
                     IEIGLHNYEARDRLKEYGPNEAVVERKQSWVKKLFKAFYNPLVVLLLGLSVVSLFIKD
                     MKSAYMMIIMVILGVSLKFFQELKADRAVEALKAMVQTTSTVIREGKSEEVKLSNLVP
                     GDIIKLSAGDIVPADVRILQAKDLFLNQATLTGEALPVEKFPASEKGSTKNPLEFSNI
                     CFMGTNIESGTALAVVATTGFDTFFGNLAKSVSQSEQKTNFDKGISQITWLMIGFMAF
                     LVPLVFLVNFFGKGNWLEAFLFSVSVAVGLTPEMLPMIVTVNLSNGAISMSRKHVIVK
                     KLGSIQNLGAMDVLCTDKTGTITQGRVVLEQHLDVDGEESDEVLEYGFLNSYHQTGLK
                     SMLDQAVLEHTEIEERLHVKENFKKVD"
BASE COUNT         2565 a         1609 c         2060 g         2350 t
ORIGIN      
        1 ctcaccggtg aaaaggggcc ataccggatt gagggcattt tagaagatgg aataaccgga
       61 gagaggctca catttgagct tggcctgacg cttgagtagg gttgaaaagc caatcatgaa
      121 aaatggtgat ggcctcacct caataccagg tgttggcaaa agcatcgcag gagatttgat
      181 tgagcttgga ataaacaggg tgtccgattt gaagaatcgc gacccacagg agctctatga
      241 tttgctttgc accaaaaaag gccatcagga caggtgttta ttgtatgttt tcaggtgtgc
      301 catatatttt gccagcaaca ccaaccacaa gccagaactt ttaaaatggt ggaactggaa
      361 agataatact gaaccaaaac gaaggagaga agccaaatga aagattgttt tatctatatt
      421 gagtggtatg tcacaaacat tgagagggcc gttaacttct acaaggggat gtttaactgg
      481 aaatttgaga aatccaccga cgattacatg atgtttgaaa tgtctaacgg agagagcggt
      541 ggaatcatgg aggttgacaa agttaaacct ggcaactcac cagtttttta cattcatgct
      601 gaccgaatcg agaattatct tgaaaaagcc ccgggccttg gcggatctgt ttccgttggg
      661 aagactgaac tctcagggat aggtttctat gcttttcttg ctgaccctga tggtaataaa
      721 ataggtcttt acgaagactt gaaacagtaa taaaatagtt accaacgatt ttgtttgggg
      781 ataataaaga atgattatta tttttaaata aatttttgca acgaaaacat tttttgtgtg
      841 tccaaggggt tgctatgggt aagaaaattt tggtgtgtta tgcaacaagg tgcggttcaa
      901 ctggtgaagt agctgaaaga atcgctagcg ttatcaaatc tgacggccac actgttgatg
      961 tgtcgcaagt caaaagaaat attgatattg acgattatga tgccgtggtt gtcgggagtg
     1021 caataaggat tggcaaatgt cttggtgagg caatagagtt tgtaaagcag agaaaacccg
     1081 atctacaaaa gaaaaagctt ttttattttg gcgtttgttt gatgattact atgccggaca
     1141 aattgcatga agtccagacc taccttgatt ccatgattgc cgagtgcaca ccagtcgccc
     1201 atgaaatatt tgcaggcaag gtagatttta gcaaactttc gttcccgata agattactca
     1261 tgaagcaaat gaagacccca gaaggcgatt ttgtggagtg gcaaaaagtc gaatcttggg
     1321 ccaaaatgat atcaaaaagc atctaatatc atcaaatata ttattattta gaataaagat
     1381 ataatttgtg cgttagagag catattaagt tattaataag catatttatt aataaaatgg
     1441 ctatttaatg catattagta gtcttaaaaa tataaaaata gaattatatc atttctaata
     1501 gatttattat ctcatattaa atcaaaaaat gatatttgta tctaaataat aatatattgg
     1561 atattaatta ggtaaaacac aatttatagc tataaaaatt gattaaatta tatttcaaat
     1621 tacttattgg tcatgttttt ggttctacat ggttgattcg tggatctttt ttgtgccata
     1681 cgatttgtaa aacatccagt gctgttattt caaattaatc tcaataataa aatgtgctgt
     1741 attttgcgat gtttgttgtt ttaatattga aagttgactc gcaattgaaa tggtttaaaa
     1801 tttttaagct gttataaatg caaggcagag ctagggcagc ctctaacaaa atccgaatcc
     1861 aaagccccca ggaggagcag tgtcttatat agacataact aaattaagag gactttcgca
     1921 ggaagaggcc gcagaaagac tggcaactga cgggccaaat gagttgccat cttctaaaag
     1981 acgcagtatt tttgtcatta tctttgaagt catcaaggaa ccgatgtttc tccttcttgt
     2041 ctcttgcggg accatatacc ttcttcttgg cgatcgccaa gaagctttca tgctcctttt
     2101 ctttgtattt gttgttatgg gaataacaat tttccaggaa aagaaaaccg agagggcgct
     2161 tgaggccctt cgtgatcttt ccagcccaag ggcgctggta ataaggaatg gtgcacagca
     2221 gaggatcgca ggaagagatg tcgtaagggg tgatttcctc attctccagg agggcgacag
     2281 ggttccagca gactgtattt taatatcgtg cataaaccta actgtcgacg aatcgctctt
     2341 gactggcgaa tcagttcctg tgagaaaaac aaattgcgat aatgaagacg agctgatggg
     2401 caagcctggt ggtgatgatc agccttatat tttctctgga acgcttgttg tttcaggcca
     2461 aggagtggca atcacaaaat caacgggctc caacaccgaa ataggaaaaa ttggcaaggc
     2521 attgcagaag cttgacaaga atgatgacac aagacttcag catgaaatct caagactggt
     2581 caggaatgtt gctttctttg gcttagccct ctgcattgtt gtttttgcaa tgtatgggtt
     2641 cacaaaaacc gattggcttg gaggactcct tgctggattg acgcttgcca tggcaatgtt
     2701 accagaagaa tttccagtgg ttttgaccat attcatggct ctgggcgcgt ggaggatgtc
     2761 aaaaaaagac gttctcacaa gaaaaatgcc agcgatagag actcttggtt cggcaaccgt
     2821 tttgtgtgtt gataaaacag ggacactgac acagaacaag atgactgttg ttgagcttat
     2881 gactggtggg gagatcattg gtctcaaggg caacgaaaaa gaattgcccg aggatttcca
     2941 cagccttgtg gaatttgctg ttctggcctc cgaaaaaaac ccttatgagc caatggacag
     3001 ggcaatccgt gaacttggcg caaaacatct tggcggaaca gaacacctac atgatgattg
     3061 ggaatttatc caggaatacc cgctttcgaa ggaattattt gccatttccc aggtgtggaa
     3121 atccacaaaa ggtgggggtt atgttattgc cgcaaagggt gcccccgagg ccattgctga
     3181 tttgtgccac ttggacaagc aagcagttga ggatataaat tcgcaagttt tcaaaatggc
     3241 ctcagaaggt ctgcgtgttc ttggtgttgc aagggcctat ttcatggagc ccggtggttt
     3301 gccaaaagct cagcatggtt ttgattttga attccttggc cttgttggtt tcttcgatcc
     3361 tgtcagggag agtgtgcccc aatcaatcaa ggaatgctat gaggcaggca taagggttgt
     3421 aatgatcacc ggtgattatc cagtaactgc atgcaaaatt gcaagatcga tcggccttga
     3481 aaactctggt gaatacatca ctggccatga acttgacgag atgtccgatg aggcattgaa
     3541 cgaaagaatt ggcaaggtta atgtctttgc cagggttgtt ccggagcaaa aattgaagct
     3601 tgtccaggcg cttaaagcca aaggcgagat cgtcgcaatg acaggtgacg gcgttaacga
     3661 tgctccagcc ttaaaggccg cagatattgg cgttgcaatg ggtggaaggg gtaccgatgt
     3721 cgcaagagag gcgtcgtcat tggtgctttt gaaagatgat ttttcatcaa tcgttgattc
     3781 tgtgagaatg gggcgaagga tatacgagaa catacagaaa gcacttgcat acatagttgc
     3841 aatacatgtt ccaatagctg gcatatcatt acttccagtg cttctgaaat ggccgacact
     3901 ttttacacca atgcatatcg tgtttatgga gcttgtaatt gacccagcct gttcgattgt
     3961 ttttgaggct gaacaggggg aaaagaactc catgaaaaga ccaccaagaa atccaaatgc
     4021 accgctcttt ggagcgggcg tattcatgct cagtgccctc caaggtgttg gtgttcttct
     4081 ggtcatcttg ctggtcaatt ttgttgccct aagaatgggc aggggcgagt ttgaggcaag
     4141 aaccctcact ttcacaacgc ttgttgtctc caaccttgcg cttattctgg tcaacagatc
     4201 gtggaacaag gccgcttttg agatagtaaa gacaaaaaat caggccttct ggtgggtgtt
     4261 ttttggggcc attgttttct tgggccttgt cctttacgtt caattccttc aaaacctctt
     4321 caagtttact acacttcacg ccagcgatat tttaatttgc ctgtgcgctg ggctcctgag
     4381 cgttgtttgg tttgaagtgt tcaaaaagct tcggaccagg aaaagaaggc ttgatgccag
     4441 agatagtaaa actggaacag ttcagtgagg aagatctaaa tagtgtcttg gtaggttata
     4501 cttctaccag aaaatacacg attgaaaaaa cagagacaaa aaaccagata acatttagac
     4561 ttgatctggt tccacttgaa agagtttatg taaagaaatg gtttttgacc aatgaggttg
     4621 atcattacag aaggatagtg ccaacaggat actccattgg tgcgtatgtc gagggcaaga
     4681 ttgtcggaat ggctattgct gaaaaccttg actggaacag aagtttattt atttgggagt
     4741 tccatgtcaa ggagggccac agaagacaaa ggattggcac tcgcatgatg atggagcttg
     4801 agaaagaggc caaacaggca aatctgagga tcatggtttg cgaggcacaa aatacaaatg
     4861 cggatgcaat tgatttttac aaacattgcg gattttcagt tgaaggcata gaccttcatt
     4921 actacgatga tcttgattca cgtaacgagt ttgccatcta catgaaaaag atcataggtg
     4981 agtaaaactg ggtcagcatt tcatggatag agtggttgca aagcgaataa tttttggatc
     5041 acacactggt gggcatacac ggggtgttta tattttctca gaaacaagta tcattctact
     5101 ggtgaaacat ggaagaaaac tttcttggtg aattcgagct ttctttaaaa gtgaacgacc
     5161 ttgtgaggtc aatggatttc taccacaaac ttggttttga gagggtagat ggagaacctg
     5221 caaaaggatg ggctgttctc cgctgcggtg atctaagact tggcctctac cagggtcatc
     5281 ttgaaacaaa cctcatgtct ttcttgggcg gcaatgtcaa atctattgca caggacctca
     5341 aatcaaagaa aattgagatg ttttctgatg ccacacagga aaaagacggc agtatgggtg
     5401 caaaacttgt cgatcctgat ggaaacatca tctatttcaa cagctttaca gacgaataaa
     5461 tgaggttacc atgcttaatt ttattgagaa atgcaggatt tacggcgcca gcagagtaga
     5521 aatatcaaag atcgcaaaat caccggccga ctgggaaaaa ttttggcgtg agcctgagtt
     5581 tggtttccca tttaaatggg gatcatgctg gaagcaatgc tgcgagtatt ttgtgtccga
     5641 ttttgaggcc gaggttgggt tttttacaga tgttcttggt tttgtatgca acgccttggg
     5701 tcaggattat gcaatgttta ccagtcctgc aagagatttt tttattgcct tctataaggc
     5761 ggaatctgac agggcaacag cgcctggagc cataaaaatc gcttttatga ttgaagagat
     5821 tgaccagact gttaggaagc tcaaagatag aggcgtcgaa tttatagatg acccccaaaa
     5881 tgttgctgaa gattcgccgc ttgtaaaggc atcctttgca acaccgaaca tgatagaatg
     5941 caccctttgg ggtttcttgg aggataaaga tgtctgctga aaataatgat atgccaatgc
     6001 attatgtaac aactcgtggc gaggccagag aaattgccaa gggcaagaaa cattactggg
     6061 tttccgattg tggatgcagg aaaggcaacg agagcggatg caagaggtca ccggttgacg
     6121 tatgcctttg ctgggcaggt ccttatggct cgactgaaga gaatgtcaga gagctaaacg
     6181 aagaagagat tgaaaaactc tttaatttgg ctgaagaaaa acatcttgtt acaaggccat
     6241 ttaggaaatg gaactcggaa acaaaatctg tcattgatga gactgacgga atctgtttct
     6301 gttgcgactg ttgttgtgca tactttaccg agccaggcga ggcttgcgac aagggacaat
     6361 caatagaaaa gacagacagg gacacgtgca cagattgtgg cgcatgtgtt gatgtttgct
     6421 attttaaagc cagaaaaatg gaagatggaa agcttgaaat agcaagcgac aattgctatg
     6481 ggtgtggttt gtgcgccgat gtttgttcct gcataaccat ggccaagagg taatctatcc
     6541 tggcaatcat attcaagaaa ctctctaaag tgcgtagttt tttgtaaaaa ctttttggtt
     6601 aaattgtctc gaaagcattg gttgacaaag tcatcctaga tgttaaattg gtattgctcc
     6661 aagaaggagc agaaggagaa ttcaaaatta tggacggtag caacagtaat aacccaaacc
     6721 cgttgcctag tggcaaacaa ccgcccgtaa gctccaagta ggtgtttttg cgcgcccctt
     6781 ggtgcctatg ggcaaaggag gcgcagcatg aaagaaatca tcaccacaaa ccgcagggta
     6841 acaaacccga tcctttcggt tatttttgga agaaccgcgt tccacaacaa aactaaaggc
     6901 aatgcttttc ttgtgctttg ggacgaaaag gaagcaaaaa gactccaggg ggaactttcc
     6961 atgaacggct ggaatgtcag actggaaact aaagatagct tcagggccta cagcaacatc
     7021 aagagcacac agccggacgt tgtggtaatt gactctaggg acaacgagcg ctcattcaac
     7081 gagctcgcag gagcactacg ctcaatcaag gcaaccaggc aaatgccaat tgttgcgatg
     7141 aactgcaaca atgaagcaaa gagccatttt gctgatgctc attcgacaag cgaaggaatt
     7201 ttggagacca tttccaggtt tcagagacaa aatctgaacc aataaaactc caaacacaat
     7261 ctgatccaac ggttttggcc ttggtgcaaa ccagggccaa atattttgtc atacctgtga
     7321 ggtcaagatg gcgaacttcg ttcttccaat cggcaagaaa acagcaaaca attatcagat
     7381 gccgccaaaa gctttggaaa ggctaacaca ggcggcaacg cttgaatcat tggatcttct
     7441 tgatctcctt gattcaaaca ttgagattgg tcttcataac tatgaggcca gagacagatt
     7501 aaaggaatat gggcccaacg aagctgttgt cgaacgcaaa caaagctggg taaaaaagct
     7561 tttcaaagca ttctacaatc ctcttgtagt tttgcttctt ggcctttcag ttgtctccct
     7621 gttcattaag gacatgaaat cagcctacat gatgataatc atggtcatcc ttggcgtgag
     7681 cctcaaattt ttccaggaac tcaaggcaga cagggccgtg gaagcactta aagcgatggt
     7741 ccaaactact tcaactgtca ttcgtgaagg caaatctgaa gaggtaaagc tcagcaatct
     7801 cgttcctggt gacatcatca aattatctgc cggagacatt gtcccggctg atgtcagaat
     7861 tttgcaggca aaagacctct ttttaaacca ggccacattg acaggagagg cattgccagt
     7921 tgaaaaattt cctgccagcg agaagggttc aacaaaaaat cctcttgaat tcagcaatat
     7981 ttgtttcatg ggaacaaaca tcgaaagcgg taccgctttg gctgtggttg caacaacagg
     8041 ttttgacacc ttttttggca atcttgcaaa gagtgtttcc cagtcagagc agaagaccaa
     8101 ttttgacaaa ggaatatcac agatcacatg gctgatgata ggttttatgg catttcttgt
     8161 acctctggtg tttctggtca atttttttgg caagggaaac tggctggagg ctttcctttt
     8221 ctcggtatcg gttgccgttg gtctgacccc ggaaatgctt ccgatgattg ttactgtcaa
     8281 tctttcaaat ggtgctatct cgatgtccag gaaacacgtg atagtaaaaa agcttggctc
     8341 aatccagaac cttggcgcaa tggatgtgct ttgtaccgac aaaactggga caataaccca
     8401 gggaagggtc gtgctggaac aacatcttga tgttgatggg gaagagtctg acgaggtgct
     8461 cgaatatggt tttttgaaca gctaccatca gactggcctc aagagcatgc ttgaccaggc
     8521 cgtgcttgaa cacaccgaaa tcgaggaaag gctccatgta aaggaaaatt tcaaaaaggt
     8581 tgat
//
LOCUS       DAOSRI010000036         8521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_7892_length_8521_cov_4.048783, whole genome shotgun sequence.
ACCESSION   DAOSRI010000036 DAOSRI010000000
VERSION     DAOSRI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7892_length_8521_cov_4.048783"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1483)
                     /locus_tag="PK717_01905"
     CDS             complement(32..1483)
                     /locus_tag="PK717_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR46454.1"
                     /translation="MKSDLQTSVGLTARMPYRLGFKAAVAGVCASESDLDTSYRLLLE
                     CLLLNPNFIPAQETIAVNRLLASEPVIALREFKNVLRTNPSSRVGLWGKGLALCILGR
                     HEDALDIILEMAENSEMAEAGSSLLKRCFGIEENSTLKVSSVVKSFTENLSTNKSKTA
                     FDLVKDIGQSDQSARLKLMLSDLLFSRGERKTAKNLLEKMLDVHPYYPGLLYKLARTS
                     KSEGEDSQTLEFLQTVADCEPAHPDPDGILSLVQSSFAQPEDIEDLMVLRQWCIDVIK
                     NTCIGVEKHEEPQLQAISLQPSEEPKNSLDPTEAEEKPEEVEPINPESPQPEKTNDKE
                     EIQKTLFGEEEPPSQEYQEKQLERTRKILEEARKMLDQTKHVQEEPQGEVGTNQSSTE
                     EEQLENIQSGEENEIFEATIVEMEVRTDEDVPSEPVIIEGGPKRIVLPTSDLEPELSE
                     SRAWSMLRDGEAEEAFLMFSKLLRKEAGIESDQ"
     gene            complement(1532..2638)
                     /locus_tag="PK717_01910"
     CDS             complement(1532..2638)
                     /locus_tag="PK717_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOR46455.1"
                     /translation="MKRISLILAIAFLVTSFAWTATGDTATTPSTELLGLINAYRATK
                     KLSPLEINTVLTKLATDHSKDMASHKKLNHNSTNGDTFAKRMEKSGLKSTYSVENIAM
                     TSSATYAKQMFELWKKSPPHNANMLNNKINSAGFGFSKSKDGYWYGTFDGAAIKKVPV
                     RRIGSFKDELCTFSRKPHYVTYKNQTGKNTKFTVKTSYKNGSGWLNPNIKSFTLPPGG
                     TQSIGCIVLSGDLKSGTYNGSVQIEWSDGIDEYAYEMTIKPSKIQLDCNVPQSISNLK
                     GTRFTVKVTVKNLGECPSKIKLMASSTVKGLSYSIKNPEITCFPNATGNSEIVVTLTQ
                     TLEGDKPFDFIASIEFASERMSVKKTITPKMAIG"
     gene            2860..4926
                     /gene="uvrB"
                     /locus_tag="PK717_01915"
     CDS             2860..4926
                     /gene="uvrB"
                     /locus_tag="PK717_01915"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR46456.1"
                     /translation="MEKSFKVVSDFSPAGDQPQAIEKLVDGIWRGMHFQTLLGVTGSG
                     KTYTMAKVIEKINRPTLVIAHNKTLAAQLYSEFSEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDLYIAKETDINEDIQRLRHSTVRSLLERRDVIVVASVSCIYGWESPEGYQEQVVA
                     VKKGDTMLRDEFVRGLVAMQYSRNNIAFEAGNVRVIGSTVDVWPPEQEDAVRVEFEFD
                     EIVKISKLDPILSSVGEEMEGFLFFPAKQFVTTQARLDYAIPMIMNELDERIKYFKES
                     DKPLEAERIEERTKYDLEMLKEIGYCNGIENYSMHLSGRQPGERPYTLMNFFQDDFLM
                     IIDESHITLPQIRGMYAGDRARKKVLVDYGFRLPSAMENRPLNWDEFQDFMHQAVFVS
                     ATPSDFEISSSEQVVEQLIRPTGLLDPEIEVKPIKGQIDDLKKEIETRVAKNERVLVT
                     VLTKKMAENLSDHLLSQGFKVCYLHSDIGTIERVEILKKLREGTFDVVVGVNLLREGL
                     DLPEVSLIAILDADKEGFLRSSTSLIQTVGRAARNVSGKVIMYADEVTNSMKTAIEET
                     NRRRAIQNEFNIEHNIKPETIKKAIRELLNIGTAQQESKLSDAMTEKDIQAMILQLTD
                     EMNLAAAMLEFEKAAKIRDQVAILRGELLVKGKGFDESSSRTKTAKKLKQKLTQAEFR
                     KKNRGR"
     gene            5180..6016
                     /locus_tag="PK717_01920"
     CDS             5180..6016
                     /locus_tag="PK717_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOR46457.1"
                     /translation="MHIVVLIKQVPDTQEVKIDPEKNTIVRAGVESIVNPFDMNATEL
                     ALQLREQYGGKVTVITMGPPQAAMALKETFSMGVDKVILLQDRGFAASDTLATSYVLA
                     QGIKRLEADGEKVDLVICGKQAVDGDTAQTGPGVACRLSFDQITYVTQVDKIDLDTRT
                     IVARRRLAKGVQVVQAQLPALLTVEKDINEPRYATLRGIIAAERVKVVEWCAADIKAD
                     AEQIGFKGSPTWVKRIFTPPPREGGPKFDGPDAVKKALEVVLDNQHFKEAVFGEEGVC
                     KR"
     gene            6013..7026
                     /locus_tag="PK717_01925"
     CDS             6013..7026
                     /locus_tag="PK717_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR46458.1"
                     /translation="MSDQEWKGVLVFIEVTDGKVQEVGWELLGEAKKLASKINQNVQA
                     LIIGHDVEKFAKDAIAYGADVVYVVDDPGYKNYITKTYAKAFVEIVNKHKPDIALIGA
                     TNLGRDLSGAIATVLKAGLTADCTGLDIDDQSKKLLSTRPTFGGNIMATIFCQKGKPQ
                     MSTVRPKVMKMPEPDPKRQGHVIKESVPVDPKDLTVKILEYIPDSCLESVEIEHAKIL
                     VAGGRGCNTPAGLDKLKELAKLIGGELCGSRPLVEAGAITPDRQVGQTGKTVVPRVYI
                     AVGISGAIQHLVGMQNSDCIIAINSDPNAPIFKVATVSIVGSWQAIVPAVIEELKKRM
                     GRQ"
     gene            7026..8327
                     /locus_tag="PK717_01930"
     CDS             7026..8327
                     /locus_tag="PK717_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR46459.1"
                     /translation="MEETFDLVIVGGGPAGMSAAIVAARAGIKTIVIERGEFPGSKNV
                     YGGVVYVHEIEKILPDFWKDKTAPIERVITDSRIVFANDHGAMQVGYKTDRFSDPPNV
                     ITTHRAKWDRWYANKAKEAGVLIVNNTVVEEVLRDLTGRVTGVRTGRPDGDLYCKLVI
                     FADGAQSPLLVRAGIRKEVKAEHVALSVKETIQLSEEEVTKRFGAKPGEGVTFEILGS
                     LADKMDGVAIMYTNKDTVSLGIGANLEQWKKSKVTPYEMIDKLKEHPMIKPLVDGGQT
                     KEYVAHWLPEGGYDTIPKLYGNGYLLCGDTAMLFNALHREGTNLAQETGRLAGEMAVK
                     AIKEGDTSESMLSEYDANVKNSFVLKDMRKYRKFPHLLFNNPMIFQTLPDVVGDMIEE
                     MLLVDGKSKKEKQKIIVRNIRKKVGLYRLMGLALRAWRSVK"
     gene            8324..>8521
                     /locus_tag="PK717_01935"
     CDS             8324..>8521
                     /locus_tag="PK717_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR46460.1"
                     /translation="MSKIEDKLYTTRYRVDHNTHLKIADMEVCRKCEGKPCTIFCPAK
                     VYIWDEDHIRVVYEGCLECGSC"
BASE COUNT         2273 a         1892 c         2132 g         2224 t
ORIGIN      
        1 aaagtcttta aaacccttca attcctcgag ttcactggtc agactcaatg ccggcctctt
       61 ttcttaggag ttttgagaac atgaggaagg cctcttctgc ttccccgtcg cgaagcattg
      121 accaggccct tgactcgctt agttctggtt caaggtccga agttggaaga acaatccttt
      181 tgggcccacc ctctattatt accggttcag atggaacatc ctcgtcagtg cgcacttcca
      241 tctcaacgat ggtggcctca aagatttcat tttcttcgcc tgattggata ttctctagct
      301 gctcttcttc agttgaactt tggttggttc caacttctcc ttgtggttct tcctggacat
      361 gttttgtctg gtcaagcatt tttcttgctt cttccaggat ttttctggtt cgttcgagct
      421 gtttttcctg gtactcctgg gaaggcggct cttcctcgcc aaatagtgtt ttttgtattt
      481 cttctttgtc gttagttttt tctggttgtg gtgactctgg atttattggt tcaacctctt
      541 caggtttttc ctctgcttct gttgggtcta aggaattttt gggttcttcg gaaggctgga
      601 gcgagattgc ctgcaattgt ggctcttcgt gtttctcaac accgatgcag gtgtttttta
      661 ttacatcaat gcaccattgc ctgaggacca ttaggtcttc aatatcctcg ggttgcgcaa
      721 atgatgattg cacaagagac aagattccgt ctggatcagg gtgtgctggc tcacaatcgg
      781 ccaccgtctg caaaaattcc agtgtctgtg agtcttcgcc ttcagatttg cttgttcttg
      841 ccagtttata caggaggccg ggataatacg ggtgaacatc cagcattttt tccaggagat
      901 ttttggctgt ttttctttcg ccccttgaga acaggaggtc ggagagcatt agcttgagcc
      961 ttgcgctttg gtctgactgg ccgatgtctt tgaccaggtc aaaagctgtt tttgatttgt
     1021 tggtggacag gttttccgtg aacgatttta ccacagagga gactttgagg gttgagtttt
     1081 cttcaattcc aaagcacctt ttcaggagac ttgagccagc ctctgccatt tccgagtttt
     1141 cggccatttc caggatgata tcaagcgcat cttcgtgtct tcccagtatg cagagtgcca
     1201 gccccttacc ccagaggccc acccttgaag acgggtttgt gcgcaggaca tttttaaatt
     1261 cccttagcgc aatgactggt tcgctggcaa ggagtctatt tacggcgatg gtctcctggg
     1321 ccggtataaa attggggttt aataaaaggc actcgaggag taatctgtat gatgtgtcta
     1381 agtcgctttc tgaagcgcag accccagcaa ccgcagcctt gaaaccaagg cgatacggca
     1441 tcctggcagt caggccaaca gacgtttgga ggtctgattt cattctaaaa gtagatatta
     1501 gctgcgaaac tatttactgt caatgttttt gctaacctat tgccatcttt ggtgtgatgg
     1561 tcttttttac agacatcctc tctgacgcaa attcgattga tgctatgaaa tcgaaaggtt
     1621 tgtcaccctc aagcgtttgt gttagcgtta cgactatttc agagtttcca gttgcgtttg
     1681 ggaaacaggt tatttcagga tttttaatgg aatacgagag gcccttgact gtcgaggagg
     1741 ccatcagctt gatttttgac ggacattccc caaggttttt tacggtcact tttactgtga
     1801 acctggtccc cttcaggttt gaaatgcttt gcgggacatt acaatccagc tggatttttg
     1861 agggttttat tgtcatctcg tacgcgtact catctatccc gtcgctccat tcaatctgga
     1921 cagagccatt gtatgtccct gatttgaggt ctccgctcaa aacaatgcac cctatagact
     1981 gggtcccccc aggtgggagt gtgaaactct tgatattcgg attcaaccat ccactgccat
     2041 ttttgtaaga agtcttaacg gtaaattttg tgtttttgcc ggtctggttt ttgtatgtga
     2101 cataatgggg ttttcttgag aaagtacaaa gctcgtcttt gaaagagccg attcttctga
     2161 ctggcacctt ttttattgcg gcgccgtcaa aggttccata ccagtaaccg tctttgctct
     2221 ttgagaaccc aaaacctgca gagtttatct tgttattgag catgttggca ttgtgtggcg
     2281 gtgatttttt ccagagctcg aacatctgct ttgcgtaggt tgcacttgaa gtcatggcaa
     2341 tgttctcgac tgaataggtc gatttgagcc ctgatttttc catcctcttt gcaaatgtat
     2401 caccgtttgt gctgttgtga tttagtttct tgtgcgaggc catatccttg ctgtgatcgg
     2461 tggccaattt tgtcagtaca gtgttgattt caagcggaga gagttttttt gttgctctgt
     2521 acgcatttat gagaccaaga agttctgtcg agggggttgt tgcagtatct cctgttgctg
     2581 tccatgcaaa tgatgtcacc aaaaaggcaa ttgccaaaat gagagaaatt cttttcatgt
     2641 atctccttaa accgcaaaat ggagtgtggt tcctttttga gatatggtat atggaattgt
     2701 tctttgtcgg ttaaaatatc aattgaatct ggattggaaa gtattttatg ccatagcaag
     2761 aatcgttgtt cccattccat gacatttttg gtgtcaagcg agaagatatc actgcccgtt
     2821 agttgcccct tcctggtgtt tggaggtata tatccactcg tggaaaaaag cttcaaagtt
     2881 gtttcagatt tcagccctgc cggagaccag ccgcaagcta tagagaaact tgtagatgga
     2941 atctggagag gcatgcactt ccagaccctg ctcggtgtca ctggttctgg aaagacatat
     3001 accatggcca aggtcataga gaagatcaat agaccaacac ttgtgattgc acacaataaa
     3061 acactggcgg cacaacttta ttctgagttt tcggagtttt tcccggaaaa tgcggttgag
     3121 tattttgttt catactacga ttactatcaa ccggaagcgt atgttccgag ctctgatctt
     3181 tacatagcaa aagagaccga catcaacgaa gacatccagc gcctccgcca ttcaacagtc
     3241 cgttcgctcc tggagcggag ggacgtaatt gtggtcgcct ctgtttcctg tatttacggt
     3301 tgggaatcgc cagaaggcta ccaggaacag gtcgtagcag taaaaaaggg agacacgatg
     3361 ctgagggacg agtttgtcag agggcttgtc gccatgcagt actcaagaaa caacatcgct
     3421 tttgaagccg ggaacgtcag ggtgatcggc tcgacagttg atgtatggcc acctgaacag
     3481 gaagatgctg tcagagttga gtttgagttt gatgagatag tgaaaatatc gaagcttgat
     3541 cctattcttt cctctgttgg cgaggaaatg gagggttttt tgttcttccc ggcaaaacag
     3601 tttgttacca cgcaagccag actcgactat gcaattccca tgatcatgaa cgagcttgat
     3661 gagagaatca aatacttcaa ggagagtgat aaaccgcttg aggcggaaag gattgaggaa
     3721 agaacaaaat atgaccttga gatgctcaag gaaatcggtt actgcaacgg cattgaaaat
     3781 tacagcatgc acctctccgg aagacagcct ggagagagac cttataccct gatgaacttc
     3841 tttcaggatg attttctgat gattatagac gagtcccaca taacgcttcc acagatcaga
     3901 ggaatgtatg ctggggacag agccaggaag aaggttctgg tcgactatgg tttcaggctc
     3961 ccatctgcta tggagaacag accgctcaac tgggatgagt tccaggactt catgcaccag
     4021 gcggtttttg tttctgcaac accgagtgat ttcgagattt ccagttcaga gcaggttgtt
     4081 gaacagctca ttaggccgac tggtcttctt gatcctgaaa tagaggtaaa accaataaaa
     4141 gggcagatag acgacctcaa aaaggagata gagacaagag ttgccaaaaa cgaaagggtg
     4201 cttgtgacgg ttctgactaa gaaaatggcc gagaatttgt ctgaccatct cctttcgcaa
     4261 gggttcaagg tttgctacct ccactctgat attggtacaa ttgagagagt tgagatatta
     4321 aagaaactca gggaagggac ttttgatgtt gtggttggcg tcaatttgct cagagaaggg
     4381 cttgatctcc ctgaagtgtc actcattgcc atacttgatg ccgacaagga aggattcctc
     4441 aggtcatcaa cttctttgat acaaactgtt ggaagggccg ccaggaatgt ttctggaaag
     4501 gtcatcatgt acgctgatga agtgacgaac tccatgaaga cggcaatcga ggagacaaac
     4561 aggagaaggg ccattcaaaa cgaattcaac atagaacata atatcaaacc tgagacaata
     4621 aagaaggcga ttagagaact gctaaacatt ggaacggccc agcaggaatc gaagctctct
     4681 gatgctatga cagagaaaga catacaggca atgattctcc agcttactga cgagatgaat
     4741 cttgccgcag caatgctgga gtttgaaaaa gccgcaaaaa tcagggacca ggttgccatt
     4801 ctgaggggtg aactgctggt caagggaaaa gggtttgatg aatcctcatc gcgaacaaaa
     4861 accgcaaaaa aactcaagca aaagctcacc caggccgaat ttaggaaaaa aaacagagga
     4921 agataaaaag accagttgtt tttttcacga attacttttg tgtttcaagt taagccaaga
     4981 cgtgattttg caccttccaa ttttgccaat tttcacgttt tagactaatt ttaatctcaa
     5041 tattcaaaac aggggtaatt cctatcttgc agggcaaacc tttgttgcta ttgtaatttt
     5101 tatgtgaaat ggtgctctaa tctgttgctt gttggagtca ctcgacaagg cttcagaaaa
     5161 gcatccagga ggtaaagcta tgcacattgt tgtgcttatt aaacaggtgc cggacactca
     5221 agaggtcaag atagacccgg aaaagaacac aattgtccgt gcaggcgttg aatcaattgt
     5281 caatccgttt gacatgaatg ctacagagct ggcacttcag ctccgcgagc agtatggtgg
     5341 aaaagtcaca gtaattacga tgggaccgcc acaggctgcc atggcactca aggaaacatt
     5401 ctcaatgggc gttgacaaag tcattcttct ccaggataga ggatttgcag cttcagatac
     5461 ccttgccact tcttacgttc ttgcacaagg aataaagaga cttgaagccg atggcgaaaa
     5521 ggtcgatctg gtaatctgtg gcaagcaggc tgtcgatggt gatactgccc agacgggccc
     5581 aggtgttgct tgcaggctca gttttgacca aataacctat gtgacccagg ttgacaagat
     5641 agatctggat acaagaacaa ttgtcgcaag acgcaggctt gcaaagggtg tgcaggttgt
     5701 ccaggcccag ttgccagcac tcctgactgt cgagaaagac atcaacgaac caaggtacgc
     5761 tactctcagg ggaattattg ctgctgagag ggtaaaagtt gttgaatggt gtgccgcaga
     5821 tattaaggcc gatgctgaac aaattggttt caagggaagc ccgacctggg taaaacgcat
     5881 ctttacacca ccgccaagag aaggtggacc aaagtttgat ggtcccgatg ccgtcaagaa
     5941 ggcattggag gttgttctcg acaaccagca tttcaaagag gcagtgtttg gtgaagaagg
     6001 ggtgtgcaag agatgagtga ccaggaatgg aaaggcgtac tcgtatttat tgaagtaaca
     6061 gatggcaagg ttcaggaagt tggctgggaa ttgcttggcg aggcaaagaa actcgcctcc
     6121 aagataaacc agaacgtcca ggccctcatt attggccacg atgtcgaaaa atttgcaaaa
     6181 gacgccattg cttatggtgc tgacgttgtt tatgtcgttg atgatcctgg ctacaaaaat
     6241 tatataacaa agacatacgc aaaggcattt gttgaaattg taaacaagca caagccggac
     6301 atcgccctca ttggggctac gaacctcggc cgtgacctct ctggtgccat tgcaacagtt
     6361 ctcaaggctg gccttaccgc agactgcact ggcctggaca ttgatgacca gtcaaaaaag
     6421 ctcttatcca caaggccaac atttggtgga aacatcatgg ccactatctt ctgccagaag
     6481 ggcaagccgc agatgtcaac ggttcgcccg aaggtcatga agatgccaga accagaccca
     6541 aagagacagg gacatgtaat aaaggaatct gtgccggtag atccaaaaga cctcaccgtc
     6601 aagatacttg aatatattcc agactcttgc ctggaaagcg ttgaaatcga gcatgcaaaa
     6661 atactcgtgg ctggtggaag aggatgcaac acaccggctg gcctcgataa gctcaaagag
     6721 cttgcaaagc tgatcggtgg agaactttgt ggttcaagac cgcttgtcga agcaggcgca
     6781 ataactcctg accgccaagt cgggcagact ggaaaaaccg ttgttccaag ggtatacatt
     6841 gctgttggaa tctccggagc aatccagcac cttgttggaa tgcagaactc tgattgcatc
     6901 attgcgatca actccgaccc gaacgctccg atattcaagg tcgcgaccgt ctcgattgta
     6961 ggctcatggc aagccattgt ccctgccgtg attgaagagc tcaagaagag aatggggagg
     7021 caataatgga agaaaccttt gatctggtaa tcgttggagg cgggccggca ggcatgagcg
     7081 ccgcaatcgt tgcggcaagg gccggaatca agacgatcgt tatcgagagg ggagaattcc
     7141 ctggctcgaa gaatgtctat ggcggtgtcg tctatgtcca cgaaatcgaa aagatcctcc
     7201 ccgatttctg gaaagacaaa actgcgccaa tagagagagt catcaccgac tcaaggatcg
     7261 tttttgccaa tgaccatggt gcaatgcaag tgggttacaa gacagaccgt ttcagtgacc
     7321 cgccaaacgt catcacgacc cacagggcaa aatgggaccg ctggtatgcc aacaaggcaa
     7381 aagaggccgg cgttctgatt gtaaataaca ctgttgtcga agaagttctc agggatttaa
     7441 ctggaagagt gaccggtgtc cgcaccggca ggccagatgg cgacctttat tgcaagctgg
     7501 tcatctttgc cgatggcgct cagtcaccgc tccttgtcag ggcaggcatc cgcaaggaag
     7561 taaaagccga gcacgtcgcg ctctccgtca aggaaaccat ccagctctcg gaagaggaag
     7621 tcacaaagcg ctttggagca aagcctggcg aaggcgtgac ctttgaaatc ctcggctcgc
     7681 ttgccgacaa gatggacggc gtggcgatca tgtacaccaa caaggacaca gtctcccttg
     7741 gaatcggcgc caacctcgaa cagtggaaga aatccaaagt caccccgtac gagatgatcg
     7801 acaagctcaa agagcacccg atgataaagc cgcttgttga tggcgggcag acaaaggaat
     7861 atgtcgccca ctggctcccg gagggtggat acgacacaat accaaaactc tatggcaacg
     7921 gctatctgct ctgtggcgac acggcaatgc tcttcaatgc cctgcaccgc gaagggacca
     7981 acctggcgca ggaaacagga aggctggccg gagagatggc agtaaaggcc atcaaggaag
     8041 gcgacacctc ggagagcatg ctttcggaat acgacgcaaa cgtcaaaaat tcctttgtcc
     8101 tcaaggacat gcgcaaatac agaaaattcc cgcacctcct gttcaacaac ccaatgatat
     8161 tccagaccct gccagacgtg gttggcgaca tgatcgaaga gatgctcctt gtcgatggca
     8221 agtccaaaaa ggaaaaacag aaaatcatag tcaggaacat aaggaaaaaa gtcggtctct
     8281 acaggctcat gggcctggcg ttgagagcat ggaggtctgt gaaatgagta aaatagaaga
     8341 caaactctac acaacaaggt atcgcgtcga ccacaacacc cacctgaaga tcgccgacat
     8401 ggaagtttgc aggaaatgcg aaggcaagcc ctgcacaatc ttctgcccgg caaaggtcta
     8461 catctgggac gaagaccaca tcagggtggt ctatgaaggt tgcctcgagt gcggctcctg
     8521 c
//
LOCUS       DAOSRI010000037         8224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_8240_length_8224_cov_5.276533, whole genome shotgun sequence.
ACCESSION   DAOSRI010000037 DAOSRI010000000
VERSION     DAOSRI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8240_length_8224_cov_5.276533"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1320)
                     /locus_tag="PK717_01940"
     CDS             complement(73..1320)
                     /locus_tag="PK717_01940"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOR46461.1"
                     /translation="MKVYQLNNESVNGSIEITDSELLYAKAQDGLLELSVSLGLEVMR
                     MMLEEDVAQYAGPKGKHKTAGRTGYRHGTEKTTVVMGGSKVRADRPRVRALDGGELPL
                     PTLGLFQNEDPLNQAILSKLLAGISVRKYARTVEGGADSVCASKSEVSRRFVEGMDVL
                     MQEFFTRKLDQDYPVMMIDGLELGKMTILAAMGIDSDGKKRVLGISEGGSENSVVVKG
                     LLEDLISRGLEADRPRLYVLDGGKALHKAVLDTFGKSAVIQRCQVHKKRNVLSYLPES
                     AQANASMAITMAYREFDYDAAKSKLLAIASEFEYKYPKAAASLLEGLDETLTVHRLKV
                     PGALRETLCSTNPMESANSACRGVIRRVCNFRDGGMALRYAAAGFIEAEHGFRRVKGY
                     RQLPILSSMLSAQYARTLETRTA"
     gene            complement(1683..3842)
                     /locus_tag="PK717_01945"
     CDS             complement(1683..3842)
                     /locus_tag="PK717_01945"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HOR46462.1"
                     /translation="MGKHFEFDKISNIVRARFVFLLCLIIILSSAGISQMAFAFPKLF
                     DNGLNPIPYSPKKPDIPKISNYQIKKDGFLINVNRNTQLAKPFSKKIATTDINSRLNA
                     FGSCVSLSVSGGVTTLIDNTFRLASSWQNSSSKYSLVMYPQIRDIIPPNAIINSVTLV
                     TDIIDPNLPSSVQVNVNEITSTWDPRDFQVGNMPSISSITAASGVWDLTSADLAYRTL
                     DITSLAGGWFSGSNQWGIAISTPITEGVELDFSLNPCPRIYIDYDLPPDSYTGNIDGG
                     TWYSDSVCPGLWLPKRATYFNKLGVLQNDVKTRVFFDTSSKIPPFGFDNQSFRYVVGG
                     NLYNNNDTNKVMELGVRTWSKVSFDMLGFPFHDIPVTQPPQVVGPPPTPMTLPQCEEE
                     IDDDGTSYPYGEIGNQIIDYSSNISYVDIPVNTGWGQIWLKKGRRIRTGPHGNLKSTV
                     YKPFTKPEMYIINDPTIVELVPRTNVPQNGSYDTSLPQPEFEQSIDIGADGPREDKME
                     QYFSGSQANWGTQSYEINPLNAAFELNNNDFSIPCHGGLSLAFGRTFSSASQSASGIL
                     GPGWHTNIEQKIMYFSEGYLHYLCASGEEYVFTPRKDSAGNVISYDPPPMLDWKVELF
                     GSGDNMHVEITDKLGDKRLIFNTNGTISDIITLQGGDIHFSWNQGKIEYIEDVYSGRK
                     LTFAYDASNRLVSVIGPDNNRNWTYEYDTQGGSIALD"
     gene            complement(4117..4193)
                     /locus_tag="PK717_01950"
     tRNA            complement(4117..4193)
                     /locus_tag="PK717_01950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4157..4159),aa:His,seq:gtg)
     gene            4291..4881
                     /gene="grpE"
                     /locus_tag="PK717_01955"
     CDS             4291..4881
                     /gene="grpE"
                     /locus_tag="PK717_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOR46463.1"
                     /translation="MKPKSAKGGKMQEKPEHENNAPDQENGALEQVVKLQETLEKVKS
                     LEEEIDKLKSSLSAQLMDNARIIQIANREKENIAQKTRKEVANLLIPVADDLERAMSM
                     LGETGQDASCRAPVEFILKSLSNALLQMGLKVVDPMGEKFDPHKFELGGQEEGKGEED
                     TVCRVLRKGYSIGEEIIRPALVVCITKSNHQKEENQ"
     gene            4878..6743
                     /gene="dnaK"
                     /locus_tag="PK717_01960"
     CDS             4878..6743
                     /gene="dnaK"
                     /locus_tag="PK717_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOR46464.1"
                     /translation="MSKIIGIDLGTSNSASAVVIGGKPTIIPAAEGVSVGGKTVPSYV
                     AFTKDGQMLVGEPARRQAALNPENTITAIKRKMGTNYKVNVFGKEYTPQQISAFILQK
                     IKRDAEAYLGEPVSKAVITVPAYFNDDQRQATKDAGAIAGLEVLRLINEPTAAAFAYG
                     VDKGDKEQKIMVFDFGGGTLDVTIMDFGRGVFEVRSTSGDTQLGGTDMDNILINYIMD
                     EFKHDTGIDLRGNKDVTYRLKEAAEKAKIELSTTLETQINLPFIASDASGPKHLMLTI
                     TRTKLEQLVRPIIEKCRAPMEQAIRDANLTPSQIDKILLVGGPTRMPITQKFVEDFMG
                     KAPERGIDPMECVSLGAAIQGAVLAGEVKDVILVDVTPLTLGIETLGGVLTKMIDRNT
                     PVPTKQTQVFSTAADNQTQVEIHVLQGERPMAGDNLSLGKFILDGIPPAPRGIPQIEV
                     TYEIDSNGIVNVSAVDKASGKKQSIRIENANKLDKNDIEKMKKEAETYSDADKARREK
                     VELKNKSDTLIYTARKSAKDAEGKVDAEKIKKVNDLADDVEKMIKEDQTDNLQSRVSE
                     LESALSDLGQQMYQQAQAQQGQQTQQEQPGPGTQDQGGNDNGEGPTINGEGRVKE"
     gene            6851..7933
                     /gene="dnaJ"
                     /locus_tag="PK717_01965"
     CDS             6851..7933
                     /gene="dnaJ"
                     /locus_tag="PK717_01965"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOR46465.1"
                     /translation="MAAKDYYDVLGVGRNATEDEIKDAYRKLVRQYHPDTNKDNPDAE
                     KKMAEINEAYTVLSNKEKRAKYDQFGQVPPEGAGFPGGGYGVNFTDDLGSFADIFDTM
                     FGGGGRSQRPRGQVVYEGADIRTDVSITLQEAFSGKTILIDLQRFDKCPECEGTGAAK
                     GTKPETCPDCRGSGQVKRTQQTFLGTFQTVGTCPRCGGSGKIISSPCPKCKGETLIKA
                     THKIEVKIPAGIEDGMRVRLAGQGDSGKNGGRPGDLYVMVHVKLDKIFTREGANIYSQ
                     VTISMYEAALGAEITIYTIDGKEKITIKPGTQPGDTMTLRSKGMPYLNSSRRGDHIIN
                     FKVTVPKKLSQKEKSLLKQIMGIKEE"
     gene            7935..>8224
                     /locus_tag="PK717_01970"
     CDS             7935..>8224
                     /locus_tag="PK717_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46466.1"
                     /translation="MEKLLFGTGGAPLSTTGSGSEAGIKRIRELGLSCMEIEWVQSSP
                     SAKESKTLSGIREEAEKEPKIVLSCHASYYINLAGEPEVIGASKDRIQRSAKA"
BASE COUNT         2486 a         1850 c         1867 g         2021 t
ORIGIN      
        1 agaaagtgtc ccaaccgtcg tagaatttgc tcaaatatag aattgctcca aatatcttct
       61 gaaaggtcag gattacgcgg ttctggtttc taaagtgcgt gcatattgag cggacagcat
      121 ggaggaaagg atcggcagct gacggtatcc tttgaccctt cggaagccgt gttccgcctc
      181 gataaagccg gcggcagcgt aacgcagggc catcccgccg tcccggaaat tgcagacccg
      241 gcggataacg cctcggcacg ccgagttggc cgattccatc ggattcgtgc tgcacagcgt
      301 ttcccgcagc gcgccgggga cctttaaacg gtggactgtt aaagtttcgt ccagcccctc
      361 taaaaggctc gccgccgctt tcggatactt atactcaaat tctgaagcaa tagccaggag
      421 cttgctcttg gcagcatcat aatcgaattc cctgtaggcc atggttatgg ccatggacgc
      481 gtttgcctgt gccgactcgg gcagataaga aagcacgttg cgtttcttgt gaacctgaca
      541 gcgctgaatc acagcgcttt tcccgaaggt atccagaact gctttgtgga gggctttgcc
      601 tccatccaga acataaagcc ttgggcggtc ggcctcgagc ccccggctga tcaaatcttc
      661 gagcagccct ttgacaacaa cgctgttttc tgagccgccc tccgagattc ccaagacgcg
      721 ctttttgccg tcgctgtcga tccccatggc ggcaagtatg gtcatcttac caagttccag
      781 gccgtcaatc atcatgacgg gataatcctg atcgagcttt cgcgtgaaga attcctgcat
      841 caagacatcc atgccctcga caaaccggcg gctgacctcg cttttgctgg cacataccga
      901 gtcggcgccg ccttctacgg tgcgggcata tttccggacg ctgataccgg caagcagctt
      961 tgacaggatt gcctgattca aaggatcttc attttgaaat aatccaagcg ttggcagcgg
     1021 cagctcgcct ccgtccaggg cccgcactct gggacggtct gcacggacct tcgacccgcc
     1081 cataacgacg gttgtctttt ctgtcccgtg gcggtagcct gtccggccgg ccgtcttatg
     1141 ttttcctttc ggcccggcat actgcgcgac atcttcctcc agcatcatgc gcatgacctc
     1201 aaggcccagg gacaccgaca actcgagcaa accgtcctgc gctttcgcat acagcaattc
     1261 gctgtcggtt atttcgatac ttccattgac ggattcgttg ttgagttgat atactttcat
     1321 tgtgacagct cctttgtcat tctgattgtt tattgccttt ttaactttat tctatcagaa
     1381 ttacttggag ctgtctcttt caatttctac ggagtttggg acagtttcta gcggaaaaaa
     1441 atataagttt tgtcatggaa aaaaataatt ttatgttgag acgatggcaa tcctgacttg
     1501 ttcccacgga gtgtagtctt gaggcggtcg gaaaatcttt tagattgcat ataataagta
     1561 ggagccgcct catagacatt tttccgagaa cggacaaggc tatattttga ctacatttca
     1621 ggcggtttta cccttggata aatttccgca tacagccgga acgtatatgc agcatccaga
     1681 catcaatcca gagcaatcga gccaccttga gtatcatatt cgtaagtcca atttctatta
     1741 ttatccggac cgataacact taccaaacga ttcgaggcat cataagcaaa tgttaacttt
     1801 cttccagaat atacatcttc aatatactcg atttttcctt gattccatga aaaatgaata
     1861 tcaccaccct gcaatgttat tatatcagat attgttccat ttgtgttgaa aataagcctt
     1921 ttgtctccaa gtttgtcagt aatttcgaca tgcatgttat cgccactacc aaaaagctcc
     1981 actttccagt caagcattgg tggagggtca tagctaataa cattgccagc tgaatctttc
     2041 cttggtgtaa atacatactc ttcgcctgat gcgcacaaat aatgcagata cccttcacta
     2101 aaatacataa ttttttgttc tatatttgta tgccagccag gccccagaat tccgctggca
     2161 gattgtgatg ctgaagaaaa agtccttcca aatgccaaac ttaatccacc atggcatggt
     2221 attgaaaaat cattgttatt gagctcaaag gccgcattca acggattaat ctcatatgat
     2281 tgagtccccc aattggcctg tgatcctgag aaatattgtt ccatcttatc ttctcttggt
     2341 ccatctgcgc cgatatcgat tgattgctca aactcgggtt gtggcaaaga agtatcatag
     2401 gaaccatttt gaggtacatt tgttcgtggt actaattcaa ctattgttgg atcattaata
     2461 atatacattt ctggctttgt aaaaggctta tataccgtgg atttcaaatt accatggggg
     2521 cctgttctta tccttcgtcc tttcttgagc cagatttgtc cccatccagt attaactggg
     2581 atatctacat aagatatatt tgaagaatag tcaataattt ggttaccaat ctcaccatag
     2641 ggatacgagg tcccatcatc atcaatttct tcttcacact gtggaagtgt cataggggta
     2701 gggggaggtc ccacaacttg gggaggttgg gtaactggaa tgtcatgaaa tgggaaacct
     2761 aacatatcaa aagatacttt agaccatgtt ctaacaccca gctccattac tttgttggta
     2821 tcattgttgt tgtaaagatt gcctccaaca acatatctaa aagattgatt atcaaaccca
     2881 aacggaggta tttttgatga cgtatcaaaa aatactcttg ttttaacgtc attttgaaga
     2941 acacccaatt tattaaaata tgttgccctc ttggggagcc ataaacctgg gcatactgaa
     3001 tcagaatacc atgtgcctcc gtcaatattt ccagtgtatg aatctggtgg tagatcataa
     3061 tcgatatata ttcttggaca tggatttaaa ctaaaatcta gttctacccc ttctgttatc
     3121 ggtgtggaaa tcgcaatacc ccattgattt gatcctgaaa accaccctcc agcaagagac
     3181 gtaatgtcaa gtgttctgta ggcaaggtct gctgatgtta aatcccatac tccagatgcc
     3241 gccgtaatag atgaaataga tggcatatta ccaacctgaa aatctcttgg gtcccatgtt
     3301 gaagttattt catttacatt gacctgcact gacgacggaa gatttgggtc aatgatatct
     3361 gttaccaatg ttacagaatt aattatggca ttcggaggta ttatgtctct tatctgtgga
     3421 tacatcacta aagagtattt actgcttgag ttctgccaag acgatgccaa tctaaatgtg
     3481 ttatcaatta atgttgttac gccaccactt acactcaagc taacacaact gccaaatgca
     3541 ttaagcctag aattaatgtc tgttgttgct attttctttg aaaatggctt cgccaattga
     3601 gtgtttctat ttacattaat tagaaaaccg tcttttttta tctgataatt tgaaatctta
     3661 ggaatatctg gttttttagg agaatacggt attggattta aaccattatc gaatagtttt
     3721 ggaaaagcaa acgccatttg agagattcct gctgaactaa gtattataat taaacataac
     3781 aagaacacga atcttgcacg tacaatgttt gagattttgt caaactcgaa atgttttccc
     3841 atttttaccc catgtttgta gtgatgaata tataaatatc tgttaattta ttaaatatca
     3901 attaaataaa tataacataa aataatatta attattaatt tatactggaa ggtagtaata
     3961 ggctcaatca cacattaatt agcggcaaca atataaaaat ttaaaactag tgatacaaga
     4021 agttattaaa aagattttat ttccagagat tggaagcgat ttggcttatt atgtaactta
     4081 agaagagtga tgatgtttat tttctaagta gttttctggg gtggatgagg ggatttgaac
     4141 ccccggcctc cagttccaca gactggcgct ctaaccaact gagctacatc caccacgcgg
     4201 cagaaataat atatgttgtt tgagagcctg tcaaggctaa actcggaggg cttgacaaaa
     4261 atattcgacg gtttagaatc attttggcag ttgaaaccaa agagtgctaa aggcggaaaa
     4321 atgcaggaaa aaccagaaca tgaaaacaac gcgccggacc aagagaacgg cgcactagaa
     4381 caggtggtca aacttcaaga gactttggaa aaagtaaaat cacttgaaga agagattgat
     4441 aaactcaagt cttctctctc tgctcagctg atggataacg cccgcataat ccagattgcc
     4501 aacagggaaa aagaaaatat agcccaaaaa actcgcaaag aggtggctaa tttgctaata
     4561 cccgtggcag acgatttgga aagggcaatg tccatgcttg gagaaaccgg gcaagacgca
     4621 agctgcagag ccccagttga attcatcttg aaaagtcttt caaatgctct cttgcagatg
     4681 ggtctaaagg tcgtcgaccc catgggagag aaatttgacc cacacaagtt cgagcttggc
     4741 gggcaagagg aaggcaaagg agaagaagac acggtttgcc gagtgcttcg aaagggctac
     4801 agtattggag aagaaatcat cagaccagca ctggtcgtct gcatcaccaa aagcaatcac
     4861 caaaaggagg aaaatcaatg agcaaaataa taggaattga tcttggaact tcaaacagcg
     4921 cttctgctgt ggtaattgga ggaaaaccaa caattatacc agcagcagag ggagtttctg
     4981 ttggaggaaa aaccgtcccg tcatatgtgg ctttcacaaa ggatgggcag atgctggttg
     5041 gagagccggc cagaaggcag gctgcgctca atccggaaaa caccatcacg gccatcaaga
     5101 ggaagatggg caccaactac aaggtcaacg tgtttggcaa ggaatacacg ccacaacaaa
     5161 tatcagcatt cattcttcag aagattaaga gggacgccga agcatacctt ggcgaaccgg
     5221 tcagcaaggc agtaatcact gttccagctt acttcaatga cgaccagagg caagcaacaa
     5281 aagatgctgg agcaattgcc ggtctcgaag tcctgaggct aatcaatgag ccaacagccg
     5341 ctgcattcgc ctatggcgta gacaaaggcg acaaagaaca gaagataatg gtctttgact
     5401 ttggcggtgg aacacttgat gtaacaataa tggatttcgg gcgtggagta tttgaggtaa
     5461 ggtcaacttc cggcgacaca cagcttggtg gcaccgacat ggacaacatt ctcatcaact
     5521 acatcatgga cgaattcaag cacgacacgg gaattgatct caggggcaac aaggatgtga
     5581 cttacaggct caaagaggct gcagaaaagg caaagatcga actttccaca accctggaga
     5641 cacaaatcaa tcttccattc atcgcatccg atgcttctgg cccaaaacac ctcatgctca
     5701 caattaccag aacaaaactt gagcagctag taagaccaat catcgaaaaa tgcagggccc
     5761 caatggaaca ggccatcagg gacgcaaacc tgacaccaag ccagatagac aaaatcctcc
     5821 tcgtcggcgg gccaaccagg atgccgataa cgcagaaatt tgtcgaagac tttatgggta
     5881 aagcgccaga acgcggcatt gatccaatgg aatgtgtttc actcggtgca gcaatacagg
     5941 gtgccgtcct tgccggagag gtaaaagatg tcattcttgt tgacgttaca cctcttacac
     6001 ttggaataga aacacttggt ggcgtcctca caaagatgat tgacagaaac acgcctgttc
     6061 ccaccaaaca aacacaggtt ttctcaacgg cagccgacaa tcagacccag gttgaaatac
     6121 atgtactgca gggcgaaagg ccaatggcag gtgacaatct ctcacttgga aaattcattc
     6181 ttgacggcat cccaccggca ccaaggggaa taccccagat agaagtcacc tatgagatag
     6241 attcaaatgg aatcgtcaat gtctctgccg tggacaaagc atctggcaag aaacagtcaa
     6301 tccgcataga gaatgccaac aagctcgaca aaaacgacat cgagaagatg aagaaagaag
     6361 ctgaaaccta ctctgatgcc gacaaggcca ggagagaaaa agtagaactc aaaaacaagt
     6421 ctgacacact aatctacacc gccagaaaat cggcaaaaga tgctgagggc aaggttgatg
     6481 cagagaaaat caaaaaagtc aatgatcttg ccgatgatgt cgagaaaatg atcaaggaag
     6541 accagacaga caacctgcaa tcaagagttt cagagcttga atcagctctc tcagatcttg
     6601 gtcagcaaat gtaccagcag gcacaagcac agcagggtca acaaacacaa caggaacagc
     6661 cagggcctgg aacccaggac caaggcggca acgataatgg agaagggccg accataaatg
     6721 gagagggcag ggtaaaggaa taatcaatcc cgcctttttc atgtgaattt ggaagttgca
     6781 aggatgcagt ctttccctgg agggactgca tccttgacat tatacaagcg cgaagcaaac
     6841 taggaaaata atggctgcca aagattatta cgatgttttg ggtgttggaa gaaacgccac
     6901 agaagacgag ataaaagacg cctatcgaaa attggtcagg cagtaccacc ctgatacaaa
     6961 caaggataat cctgatgccg aaaaaaagat ggcagagatt aatgaggcat acacggtttt
     7021 gtccaacaaa gaaaaaaggg caaaatatga ccagtttggg caggtcccac cagaaggggc
     7081 agggttcccg ggtggcggtt atggcgtcaa ctttaccgat gatctggggt cgtttgccga
     7141 catttttgac accatgtttg gtggtggcgg caggagccag aggccaaggg gccaggttgt
     7201 atacgaggga gcagacataa gaactgatgt atcaataacc ctccaggagg cctttagcgg
     7261 caaaacaatc cttattgacc tgcaaagatt tgacaagtgc cccgagtgtg aaggtacagg
     7321 cgctgccaaa ggcacaaaac cagaaacatg cccggattgc agaggctccg gtcaggtcaa
     7381 aaggacccag cagacgttcc tgggcacatt ccagacagtc ggcacatgcc caagatgtgg
     7441 tggttccggg aagataatca gttccccatg cccaaaatgc aagggtgaaa cactcattaa
     7501 agcaacccac aaaattgaag ttaaaatccc tgcaggcata gaagacggga tgagggtcag
     7561 gcttgcaggc cagggcgact ctggcaaaaa tggaggcaga cctggcgacc tctatgtaat
     7621 ggtccatgtc aaattagaca agatattcac aagagaaggg gcaaacattt attcccaggt
     7681 aacaatttcc atgtacgagg cggcccttgg cgccgaaatc acgatataca ccattgacgg
     7741 caaggaaaaa ataaccatca aaccgggcac ccagcctggt gacacgatga ctctgagaag
     7801 caagggaatg ccctatctca actccagcag aaggggcgac cacatcatca acttcaaggt
     7861 gacggttccc aaaaagcttt cacaaaaaga aaaatccctg ctaaaacaaa ttatgggtat
     7921 aaaggaagag taacttggaa aaactgctct ttggcaccgg aggtgctccg ctctcaacta
     7981 ctggttccgg cagtgaagcc ggaataaaga ggatcaggga gctagggctc tcctgcatgg
     8041 agattgaatg ggtgcagagc agcccttctg caaaagaatc caaaacactt tccggaatca
     8101 gggaagaggc agagaaagag ccaaaaattg ttctctcatg ccacgcgtca tactacatca
     8161 atctggctgg agaacctgaa gtcatcggtg cgtcaaagga caggatccag cgatcagcaa
     8221 aagc
//
LOCUS       DAOSRI010000038         8212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_8255_length_8212_cov_4.331372, whole genome shotgun sequence.
ACCESSION   DAOSRI010000038 DAOSRI010000000
VERSION     DAOSRI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_8212_cov_4.331372"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK717_01975"
     CDS             complement(<1..311)
                     /locus_tag="PK717_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46467.1"
                     /translation="MKRTLAIATVLVTIFPYCPSSFSSPSGTNGVWVEDTQMSYAGTY
                     DLYCTFASGFDPEASSSTYLSVDYKPGGQGTKLTPDSKYLPIEFMVDSKNICSFEELW
                     LS"
     gene            complement(308..1045)
                     /locus_tag="PK717_01980"
     CDS             complement(308..1045)
                     /locus_tag="PK717_01980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR46468.1"
                     /translation="MDWINNYFDETYAKYFLEPVDEERTSSQVAFIENVLGISPPCEV
                     ADFCCGIGRHTVEFAKKGYLSVGYDFNDYYINCASRQALDCGLVSAFFFKQDMRNFQE
                     EGLFDVACCLWVSFGYFDDDTNRDIFFRMVRSLRSGGKFFMDLENREYILKNFIHEKW
                     RTKDEAIILERNKFDPETSRIKCNRTIIENGTKKIYTRHVRMYTATEIIQLAKASGLQ
                     EIKLLGDWDGKPYGLQTQRMILTGRLP"
     gene            1135..2475
                     /gene="dnaB"
                     /locus_tag="PK717_01985"
     CDS             1135..2475
                     /gene="dnaB"
                     /locus_tag="PK717_01985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR46469.1"
                     /translation="MLSVEPRLPPFALDMEQAILSGIMMDASAIPQVTDWITSPEDFY
                     EPRHKILYGAILEQYEGGKPVDYLAIAEKLKQDKKLEKAGGIEYLSELTTALPTAADL
                     GYYSQIVREKSLLRKVIEVGGILTSNAYQDGVKADELIDSAEKLLFEAAAHKSTFSHI
                     RDLLGPVLKRLEALSKTKAGITGLPSGFYDLDKLTAGFQTSDLIILAARPSVGKSSLG
                     LNMACNAALRSGKPVAIFSLEMSKEQLVERMIASEARVNASDMRRGMLGDDAWPRIGH
                     VMSELYNAPIYIDDSFDLSVLELRAKARKLAKEANIGMIIVDYLQLLRTPQKTENRVQ
                     EISQMTRSLKALARELDIPVVVQSQLSRSVEQRQDRRPVLSDLRESGSIEQDADVVMF
                     LSHPEAQKERDLNIIDLVLAKQRNGPVGVIKLKFLRQFTRFESLAAPDGNDFEI"
     gene            2526..3032
                     /locus_tag="PK717_01990"
     CDS             2526..3032
                     /locus_tag="PK717_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR46470.1"
                     /translation="MEDEYEKLVYLAKGGDQEAFIRLADSCKERVAKIAFYVLGNRDE
                     AEDVAQEAFLKAFVGIRSLKDGVPFDLWVSKISRNLSIDRLRKRRPQADPVEEWSKES
                     EPETILDIRKAIMELPALFRLPLTMLYFDDMPTDAIARVLGVPQGTVKSRIHKAKEMI
                     KRRLGGQF"
     gene            3019..3270
                     /locus_tag="PK717_01995"
     CDS             3019..3270
                     /locus_tag="PK717_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46471.1"
                     /translation="MDNFENEVGNARPGIDIPGPFFIPRLRVALKKIADETVANPLGA
                     CAGCAMATILVAFAFLAAIGRLDLVPDLVRTIGNVVGIS"
     gene            3267..3854
                     /locus_tag="PK717_02000"
     CDS             3267..3854
                     /locus_tag="PK717_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46472.1"
                     /translation="MRKFIGQARTAFTDLTSAISSSPSIGESIVITLLLQIFCVVLLV
                     QNALGPDATSVSPFPSPVAFSLPARIAFSFLVQILSILLPVLIFLGKKGLLLFAENLA
                     LINFIGFCACVIGLIYQSVFWPMYKLLPLTGILAKAIYESVFLVYPVLVLFIFRYVLL
                     WMLAKKTGASTSRIFLLAGSYLLIAAVMSFVGGLI"
     gene            3851..5368
                     /locus_tag="PK717_02005"
     CDS             3851..5368
                     /locus_tag="PK717_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46473.1"
                     /translation="MRKNWWVLLVGLVVFTAVYAIYFPQSQKPLRLSVDAIWRARWPA
                     ETALASAKPFIATEKAVYKVDGNTIQRIDYPVQPELPQGLSWGKPNILSMSVYDGEPV
                     LQLKNGFLRYSKGQWTPFEQTEDVGISDGWEITDGTVTIGGESGIIIGKSGSGFVEKI
                     EKEKIFFQIQGKKFEVADDRNSQNKVFSPPLSDDGELRGVVVGKNTFMPTKDGRISIM
                     ADGKETGVIDRANLGLDIVPAKIASDGETIYVMTRAIYGSSPSFWVQAYDTKEFKWKG
                     ELVASSGSKPVSVGFCSGMIVVLFDDGLLSFYNKEGFSLFNEKVCDSPLDMVSVGETI
                     YVLSSDSLLSIAVTQKSAQNRIWPRMVDLGNVAGEARFKVGVEVRDTPNLVTKGDFVK
                     LDSIEKKEGYYAINLVARTKGLVSFEEYEADIIIESGIFREIVPVKMVASPKTTKLEV
                     FEGVAIDVVAGKSYKTEIEGETLKLDMELPKSGEIIYDPLTSSAFVLDVRRGLKL"
     gene            complement(5365..6243)
                     /locus_tag="PK717_02010"
     CDS             complement(5365..6243)
                     /locus_tag="PK717_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46474.1"
                     /translation="MEKLSNLFEFEYVLNGRIASHCLSVLRRGLSKDPVHGEKISGAE
                     SFFDSITPGNDRECGKKGNMVRDDGGRWATVHQERLGRWKVRVRVSQKEGLEVTQAYL
                     DSSGTMFGYHRMLSQNYILARSRCKLGPFVVPFSKGFAVGGLFVPHHSFSVLQTCWFY
                     EDGTVVSKVTESEGLPSFYRFDYFIDVYEAMLLDKNKKWCNLLKLPVYSEYCGADREG
                     NSMMVQVDDIGVMRCGQIEGLPWECTLSRKKIPMRGPLPPKIGIPLGEKIWLNFLIKC
                     EKPVLSVLGPKMTFEI"
     gene            complement(6368..6826)
                     /locus_tag="PK717_02015"
     CDS             complement(6368..6826)
                     /locus_tag="PK717_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOR46475.1"
                     /translation="MSKIVIGLGNPILSDDAVGLRVLEALRSKNEGRATFMEANSGGL
                     ALLDMVQGFDEAVLIDSIMTGQVPPGTLMVFSLQDFSETRHASNLHDVNFATALEIGK
                     NHGLKIPDRFTIYAVEVTEVLTFSATMTLEVEVSLQKIISEIAARENLLA"
     gene            complement(6827..>8212)
                     /locus_tag="PK717_02020"
     CDS             complement(6827..>8212)
                     /locus_tag="PK717_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOR46476.1"
                     /translation="GNVDECFFQIVELRGFEEFCKGRPVEELPRIVTQLCGICPWAHH
                     LASSKALDMMFNVDPPKTAKMLREAAYCGHYIHSHISHFYALGAPDFVRGPGAPASKR
                     NVVGVIEAVGRELGAEVLHARVMAQDIQARIGGRATHPVFGLPGGISKPISKEDRAEM
                     LSQAKTLLEFAKKTVKILEAVVLSNKAYLDIITNKDLYYHESYHMGLVDENNHANFYD
                     GKVRVIDPKGKEFTKFEAKNYLEEVAEQVMPWSYLKFPYLRKIGWKGLTDGPESGLTR
                     VAPLSRLNVSKGMATPLANAEWQRFMDTLGGAPVHFSLANHWARVIELVYACERLVEL
                     LSDDQITDPKVRNIPTTNKRVGMGIVEAPRGTLIHHYESDENAMVTKINMIVATAFNH
                     GAICIDTAKAARGLIKDGMISDGILNMVEMAFRAYDPCFSCATHSLPGQMPLEVRVHN
                     SNGEITQTLRK"
BASE COUNT         1993 a         1937 c         2048 g         2234 t
ORIGIN      
        1 gaaagccaga gctcctcgaa tgagcagata ttttttgaat cgaccataaa ttctatgggc
       61 agatatttcg aatctggtgt gagctttgtg ccttgcccac ctggtttata gtcgacagag
      121 agataggtcg agcttgaggc ctctgggtca aagccggaag caaaggtgca gtataaatcg
      181 taggtgcctg cgtaggacat ctgggtatct tccacccaca caccgtttgt gcctgatggt
      241 gaactgaagc tggatgggca atatggaaag attgtgacca gaacagtggc aatggcaagt
      301 gttcgtttca tggcagtctc cctgtgagta tcatgcgctg ggtctggagt ccgtatggtt
      361 ttccgtccca gtctccaaga agttttattt cctggaggcc gcttgctttt gcaagctgga
      421 ttatctctgt tgcagtgtac atcctgacgt ggcgtgtgta gattttcttt gtgccgtttt
      481 caatgattgt cctgttgcat tttatcctgc ttgtttccgg gtcaaacttg tttctctcaa
      541 gaatgattgc ctcgtccttt gtcctccact tctcatggat gaagtttttc aggatgtatt
      601 ccctgttttc taggtccatg aagaatttcc cgccagaccg cagtgacctt accatcctga
      661 agaaaatgtc cctgtttgta tcatcatcaa agtatccaaa agagacccac aggcagcacg
      721 caacatcaaa caggccctct tcctgaaaat ttctcatgtc ctgcttgaaa aagaatgcag
      781 acaccagacc gcaatccagc gcctgccgtg aggcacaatt tatgtagtaa tcgttgaaat
      841 catagccaac agagaggtag cccttctttg caaactcgac cgtgtgcctt ccgatgccac
      901 agcagaaatc tgcaacttca caggggggtg atatgccaag gacattttct atgaaggcaa
      961 cctgcgaaga agtcctttcc tcatcgacag gttctagaaa atattttgca tatgtttcat
     1021 caaagtagtt atttatccag tccattttca tgctctaatg taattccttg cattgctaaa
     1081 tgcaacgaac aggccggttg aaaagaggga ccacaagcta taatatcttc tattatgctt
     1141 tcagttgaac ccagacttcc accattcgcg cttgatatgg aacaggcgat actctcgggc
     1201 atcatgatgg acgcttcggc gatcccccag gtgaccgact ggataacttc gcctgaagat
     1261 ttctatgagc cacgccataa aatcctgtac ggagcaatcc ttgagcagta tgaaggtggc
     1321 aaacctgttg actaccttgc aatagcagag aagctcaagc aggacaaaaa gcttgaaaaa
     1381 gctggcggaa tcgaatatct gtcagagctc acaacggccc tgccaactgc cgccgacctt
     1441 ggatactact cccaaatagt cagggaaaaa tcgcttctaa ggaaggtcat cgaggttggt
     1501 ggaatactca cgtcgaatgc ctatcaagat ggtgtaaagg cggatgaact catcgacagt
     1561 gccgagaaac tcctctttga agcggccgcc cacaaaagca ctttctcgca catcagggat
     1621 ttgctgggcc cagtattgaa gaggcttgaa gcactctcaa agacaaaagc tggcataacg
     1681 ggactacctt caggatttta tgatctggac aagcttactg cagggttcca gacatctgat
     1741 cttatcatcc ttgccgcgag gccttctgtc ggaaaatcca gcttgggact gaatatggcg
     1801 tgcaatgccg ccttgcgctc tggcaaaccc gtggccatct tctcgcttga aatgtcaaaa
     1861 gaacagctcg tggagcgcat gattgccagc gaggccaggg tgaatgcttc cgacatgaga
     1921 aggggcatgc ttggtgatga tgcctggcca cgcatcggcc atgtcatgag cgagctctac
     1981 aatgccccga tctatattga tgattcgttt gacctctcgg tcctggagct gagggcaaag
     2041 gccagaaagc tagcgaaaga ggccaatata ggcatgataa ttgtggacta cctccagcta
     2101 ctcaggacgc cacagaaaac tgaaaacagg gtgcaggaaa tctcccagat gacgcgctcg
     2161 ctcaaggcac tggcaagaga actagacata ccggttgttg tgcagtccca gctctcaagg
     2221 tcggtggagc agaggcagga ccgtcgtcct gtcctttcag acctcaggga atctggctca
     2281 atcgagcagg atgccgatgt tgtaatgttc ctctcgcacc ctgaagcaca gaaggagcgc
     2341 gatctcaaca ttatcgacct tgtgcttgcc aagcagagga atggcccggt gggcgtgatc
     2401 aagctaaaat ttcttcgcca gttcacgagg ttcgagagcc ttgccgcacc cgatgggaac
     2461 gatttcgaaa tctgatgaac cgcacacccc acttttgcgt ttgagttgtt gaaggcactg
     2521 tggggatgga agatgaatac gaaaaacttg tttatctggc aaaaggtggc gaccaggagg
     2581 cttttatcag gcttgccgac tcctgcaagg agagggtcgc aaagattgcc ttttatgtcc
     2641 ttggaaacag agacgaagcc gaggatgtcg cgcaggaggc ttttcttaag gcctttgtcg
     2701 gaatccggag cctcaaggac ggggtgcctt ttgacctctg ggtttccaag atttccagaa
     2761 acctttccat tgacaggctc agaaagagaa ggccacaagc tgaccctgtt gaggagtgga
     2821 gcaaagaaag cgagccagag actatcttgg acataagaaa ggcgatcatg gaacttccgg
     2881 cgttgttcag gctcccgttg accatgcttt attttgacga catgccaacc gatgcaattg
     2941 caagggtcct tggcgtcccg cagggaacag tgaagtccag gatacacaag gccaaagaga
     3001 tgataaaaag gagattaggt ggacaatttt gagaacgagg taggcaatgc taggccggga
     3061 attgatattc caggcccatt ttttatcccc agactgaggg tcgcacttaa aaagattgcc
     3121 gacgaaacag ttgccaaccc tctgggggcg tgtgccgggt gcgcaatggc aaccatattg
     3181 gttgcgtttg cttttcttgc ggcaataggt cgtcttgacc ttgttcccga ccttgtcagg
     3241 acgattggaa atgtggtggg gatatcgtga ggaagtttat tggccaggca aggacagctt
     3301 ttactgattt gacctctgcg atatctagtt caccttcgat tggcgagtca attgttatca
     3361 cacttttgct ccagatattt tgtgtcgtct tgcttgtgca gaacgcgctt ggtcccgatg
     3421 cgacatctgt ttcgccgttt cccagcccgg tagccttttc tctacctgcc aggattgctt
     3481 tttctttttt ggtgcagatt ttgtcgatat tgctgccagt gctgattttt cttggaaaaa
     3541 aggggctgtt gctgtttgca gaaaatctgg cgctgataaa ctttattggc ttttgtgctt
     3601 gtgttattgg cctcatctac cagtcggttt tctggccaat gtacaaatta ctgcctttga
     3661 caggaattct tgcaaaagca atttatgagt cggttttcct cgtttatcct gttctggttt
     3721 tgttcatttt caggtatgtc ctgctgtgga tgcttgctaa aaaaaccgga gccagtacct
     3781 cccgaatatt tcttcttgcc gggtcatacc tgctgattgc ggccgtgatg tcatttgtcg
     3841 gaggcctgat atgagaaaaa actggtgggt cctgcttgtc gggcttgtgg tttttactgc
     3901 cgtttacgca atctacttcc cccagagcca gaaaccgctc aggctttcag tggatgccat
     3961 ctggagggcc agatggccgg ccgaaactgc ccttgcttcg gcaaagcctt tcattgcaac
     4021 tgaaaaggcg gtttacaaag ttgatggcaa taccattcaa aggatcgatt atcctgtgca
     4081 gcccgaactc cctcaagggc tgtcttgggg aaaaccgaat atcctgtcca tgagcgttta
     4141 cgatggtgaa cctgttttgc agctcaagaa cggatttttg aggtattcaa agggtcaatg
     4201 gacacccttt gaacaaacgg aagatgtggg aataagtgat ggctgggaaa ttacagacgg
     4261 gacagtaaca attggcggag aatcggggat tattatagga aaatcgggca gtggttttgt
     4321 tgaaaaaatt gagaaggaga aaatattttt tcagatccag ggcaaaaaat tcgaagttgc
     4381 agatgacaga aacagccaga acaaggtttt ctccccaccc ctgagcgatg atggggaact
     4441 gagaggcgtt gtggttggaa agaacacttt tatgcccaca aaagatggca ggatttccat
     4501 aatggccgat ggcaaggaaa caggcgtcat tgatcgcgca aatctcggcc ttgatatcgt
     4561 accggcaaag attgcctctg acggggaaac aatttatgtc atgacaaggg ctatttatgg
     4621 gtctagcccc tctttctggg tgcaggcgta tgacaccaag gagtttaaat ggaaaggtga
     4681 actggtggcg tcatctggat caaagccagt atctgtcggt ttttgctctg gaatgattgt
     4741 tgttcttttt gatgatggtc tgctctcatt ctacaacaaa gaggggtttt cgctttttaa
     4801 tgaaaaggtt tgcgattcac cgctggacat ggtttcagtt ggcgaaacca tctatgttct
     4861 ctcgtcagat tcgctgctgt caattgccgt cacgcaaaag agcgcacaaa ataggatatg
     4921 gccaagaatg gttgatttgg ggaatgttgc tggagaggca cgcttcaagg taggtgttga
     4981 agttagggat actccaaacc ttgttacaaa gggcgatttt gtaaaactgg attcaatcga
     5041 gaaaaaagaa gggtattacg caatcaatct ggttgccagg acaaaaggcc tggtttcttt
     5101 tgaagagtat gaggctgaca tcataattga atcaggcatt ttcagggaaa ttgtgccggt
     5161 aaaaatggtc gcatcaccca aaaccacaaa acttgaagtg ttcgagggtg tggcaattga
     5221 tgttgttgct ggaaaatctt acaagacaga gattgagggt gaaaccctga aactcgacat
     5281 ggaactgcca aaatcaggcg aaatcattta tgatcctctc acatccagtg cttttgtcct
     5341 tgatgtcaga agaggtttga agctctagat ttcgaaggtc atttttggcc caagcactga
     5401 gaggacaggt ttttcgcact ttatcagaaa attgagccat attttttcgc caagcggaat
     5461 gcctattttt ggtggaagag ggcccctcat tggtatcttt tttctggaca acgtgcactc
     5521 ccatggcagg ccctctatct gtccgcacct catcacccca atgtcatcaa cctgcaccat
     5581 catggagttg ccctccctgt ctgccccgca atattcgcta tagactggca atttaagcaa
     5641 attgcaccac tttttgtttt tgtccagcaa catcgcctcg tagacgtcaa tgaagtagtc
     5701 gaacctgtaa aaagagggca ggccctcaga ctccgtcact ttcgagacga cagtgccatc
     5761 ctcataaaac cagcacgtct gcaagacaga aaacgagtgg tgtggaacaa agagccctcc
     5821 gacggcaaaa ccttttgaaa atggcacaac aaatggccca agcttgcacc gtgatcttgc
     5881 cagaatgtaa ttttgagaca gcattctgtg gtagccaaac attgtccccg aggagtcaag
     5941 gtaagcctgt gtgacttcaa gaccttcctt ctggctcacc ctgaccctca ccttccatcg
     6001 tcccagcctt tcctggtgga ctgtcgccca cctgcctccg tcgtcacgca ccatgttccc
     6061 ttttttgccg cattccctgt cattgccagg cgtgatgctg tcaaaaaaac tctctgcacc
     6121 acttattttt tccccatgta ccgggtcttt tgaaaggccc cttcgtaaga ctgaaaggca
     6181 gtgcgaagct attctgccat tcaggacgta ttcaaactca aagaggtttg aaagtttttc
     6241 catgtggcaa atgatattca tggcatgtga catcattttg tcacacaaaa aggatttttt
     6301 gtcggtttta taatttcgta ggtttaattt gcgaaattat ttttttgtgg gattttggct
     6361 gacaaaatca agccagaaga ttttctcttg cggctatttc cgaaatgatt ttttgaaggg
     6421 atacttcaac ttcgagcgtc attgtggccg aaaatgtcag cacctctgtg acctctaccg
     6481 cgtagattgt gaatctgtct ggtatcttca gcccgtggtt tttgccaatt tcaagtgctg
     6541 ttgcaaaatt cacgtcgtgc agattgcttg cgtgccttgt ttccgaaaaa tcctggagcg
     6601 aaaaaaccat tagcgtgcct ggcggcacct ggccggtcat gattgagtcg atgagcacgg
     6661 cctcgtcaaa gccctgcacc atgtcaagaa gggcaagacc accggagttt gcctccatga
     6721 aagtggccct gccctcgttt tttgacctta aagcctcaag gaccctcaac cctactgcat
     6781 catccgagag gattgggttg ccgagtccta tgacaatttt tgacaattat ttcctcagcg
     6841 tttgtgtgat ttcgccgttg ctgttatgaa ccctgacctc cagtggcatc tggccaggga
     6901 gcgagtgtgt ggcgcatgag aagcatggat cataggccct gaaggccatt tccaccatgt
     6961 tgaggatgcc atcggaaatc atgccgtcct ttatgagtcc ccttgcggct tttgctgtgt
     7021 cgatgcatat tgcaccgtgg ttgaaggctg ttgcaacaat catgtttatt tttgtgacca
     7081 tggcgttttc gtcggactcg tagtggtgga tgagtgttcc ccttggtgcc tcgacaatcc
     7141 ccatcccgac cctcttgttt gtggtcggta tgttcctgac ttttgggtca gttatctggt
     7201 cgtcggagag cagttcaacc agcctctcac aggcatagac cagctcgatg acccttgccc
     7261 agtggtttgc aaggctgaaa tgcaccggcg cgccacccag cgtgtccatg aacctctgcc
     7321 actctgcgtt tgccagtggt gttgccattc ccttcgatac gttcagccta gaaagtggag
     7381 cgacccttgt cagcccagat tctgggccgt ctgtcaggcc tttccatcca attttcctca
     7441 gatacgggaa tttgaggtag ctccatggca ttacctgttc tgctacttcc tccaggtagt
     7501 ttttggcctc aaatttggtg aattccttgc ccttcggatc aatgaccctg acttttccgt
     7561 cgtaaaaatt cgcgtggttg ttttcgtcca caaggcccat gtggtagctt tcgtggtaat
     7621 acaaatcttt gttggttatg atgtcgaggt atgccttgtt ggaaagaacc actgcttcga
     7681 ggattttgac agtttttttg gcaaactcca gcagtgtttt tgcctgtgag agcatctcgg
     7741 cgcggtcttc cttggaaatt ggcttgctga ttccgcctgg aagcccgaag actggatggg
     7801 tcgctcttcc gccgatcctg gcctgaatgt cctgggccat gacccttgca tgaaggacct
     7861 ctgcgccgag ttccctgcca acagcctcga ttacaccaac aacatttctt tttgatgccg
     7921 gtgctccagg gccgcgaaca aaatctggtg cgccgagtgc atagaagtgg gagatgtggg
     7981 aatgtatata atgtccgcaa tatgcggcct ccctgagcat cttggccgtt tttggtgggt
     8041 cgacattaaa catcatgtcc agggcctttg atgatgcaag gtggtgcgcc catgggcata
     8101 ttccgcaaag ctgggtcact atgcgtggca gttcttcaac cggcctgcct ttgcaaaact
     8161 cctcgaaacc ccttaattcc acgatctgga agaagcactc gtcaacattg cc
//
LOCUS       DAOSRI010000039         8028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_8484_length_8028_cov_4.350809, whole genome shotgun sequence.
ACCESSION   DAOSRI010000039 DAOSRI010000000
VERSION     DAOSRI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_8028_cov_4.350809"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2380)
                     /locus_tag="PK717_02025"
     CDS             complement(<1..2380)
                     /locus_tag="PK717_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR46477.1"
                     /translation="MRLKRPEENHYTQYFFRSSSFGDFFVVCFTPVEKPAAVNDFLAL
                     PDCEVDNCKPTIGDIFGSFPDALLLIDSEGKLVDCKIPNGMVLDMPWRRHVGKNVSEI
                     LPSNIAEAFLQTIGKSVFTGRTTIYEYDFDYGHGKKYFEARFSRLSNNHVLVLVRNIN
                     EKKNLLDQLSKQVEKYKTFFTHAPEPYLVLSFPDRNIVHVSAEFTDLFGYSEAEIGER
                     TPEELELWVSDAEKKKYLSMLAKDGIVSNFETLLYNKSRDAIPVLISSHVIQTNEGKR
                     YLTLVRDISSQKLSQMKLEESEKRHRKLVEGIGTPIFVHDGDKFLFYNKEFLDFIGLS
                     DQEVEKVNLYDLIVPELRAEFAFKVKRAFLGDTSVSRSDMKFVCGDNKIKDTRTNIVG
                     GNYGGKMCAYVSVSDLTSLIEAKDALVETNEFLNALVASTEDIVLAMDENGKCVFVKM
                     KPDWAGNDGGLFLGSDIEDIFGAGKSLDEFKAMVLNVAKTGTPGEFAGCFRILGKEVW
                     LNARCSPFRGPKGNIIGTISLIKDITASLVDRRKILEEQDRLDLIIANISDIVFEVRN
                     GFLSYVSPNCVKVLGRSVGEFLGRSATEFISVWKALRIQRSGRDAEFMVKLIDSDNLE
                     RVFEIKLSHIGGSYIGIARDITDSVQKEEAKRAFLGSVAHELRNPLTVISGFSELLEG
                     MVQKNPNALEVVKMIREAALREQHHVDDLVSFGVTKPRYVFKLVDAHGLFVGLQTKLK
                     ILFEESLNPMQKKTGASFIVDIDEYLKGRSIKVETGSIEEVFGNLLINAMKYC"
     gene            2830..4062
                     /locus_tag="PK717_02030"
     CDS             2830..4062
                     /locus_tag="PK717_02030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR46478.1"
                     /translation="MRKQPIFYNANFTRYVMSQLFAFPSAWIYGSALMWVTLQITNSP
                     LLLGVMGMCSSLPYLVLTPVTGSMAERYDRRKLTLVTNIINVVQSSLLAFMAFTGLIN
                     YPILAIFALINGATGAIQEPARLAFLLDMVGPEHLIQANALSSVTFNIARILGPIVAG
                     LLLTVHSGYCFALSALLLVPSIVMFTIIDVKSTKIEPRGSMIVQFVEGAKYISRQKIP
                     FLILFTIVLASTFGYGITILSSAIASDVLKLGSIGYGLLQSSVGVGALIGALVISMTK
                     SRQGIMAKFRSGSIMQAVMGLALALVLLLYPTIWLAIPLYAIFGYMMVNQNVLASSIL
                     PMVTQEGYIGRVMGFYSFAVSGVMPIGNLIAGGLTQVLTIPIGLLISYVALLIPTLII
                     CVSPKALNKDPFQNKIQS"
     gene            4059..5816
                     /locus_tag="PK717_02035"
     CDS             4059..5816
                     /locus_tag="PK717_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46479.1"
                     /translation="MRTFLAFAIATTMALGWPCAFPDGKGNPYTPTELPPGEPQVHWS
                     MTEVKSPTSACIVENRLYFASQNIANCLDLETGKKVWGAELVSNIVAPPCFVSGRAIF
                     CDKVGNVYSIDSSNGKVIDRASTSSGIVAPPIAISGLIYVSSTSGKVVSFTPDLKEQM
                     SVDLETPLSCSSIITSQGLFQQTSDGTIYVINPENGAILSNIKADPPIGQICQNDGLI
                     NIPVQSGVTRLKGDQRIPIELPSSPSCMVAVPGGPLVVGTSFGLYGISGDKLSWSMKT
                     EKPVSAVSANSEIAVFGCAGGLVAGVRLKDGEVLWKATMAGDIVHPIPIIRDGLMVSS
                     SKSVTFFKLWDLNPEPSIIDLGHVPTGEIATGLFSMSNPANSTGPVQVICTSEKPEIT
                     INPGNAIIVPGGKAFFNVVLDSTGIKEGKYSTTITIKTKTSSYKVVVGYNIVPQPFVA
                     ELKLGDSIMKMKRGTQKWEVKLDSPPFLQSNRTMVPLRAISESFGPKVTYVKDGCPGS
                     GRVDITLGGTIIYHCIGTNILTLKLPGKQPEAKTFDTASVIVAGRTFVPIRFIAEAFL
                     ATVNWDANTKTVIITYQPP"
     gene            5813..7399
                     /locus_tag="PK717_02040"
     CDS             5813..7399
                     /locus_tag="PK717_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46480.1"
                     /translation="MKKLTIFVLVLLCALSGVTSAVALIHESAGATYTFIGGSWTDKN
                     SWEPATAGNGPGLDDNVVIPEGGNAVMPKSCDIGTLTVRGKLTMGNDLRLNSMTVETG
                     GVFELGADLTCTGMLKNNGQIKGGDVFLKCQNFENNAFFIATGKTQIVAVFNIKNLGT
                     LKAGPLLMLRSNNFTNDGLLETTAKPGTVAVFINNNLANNGSIRASAGKSGSNGGAIF
                     VACSSFEGTGKVYPGDGGNGNPPGKKGVALIGSSFDQTGWTNWEPTAPKGNPPQNLTA
                     WNAQVVQAKDGKSSISTGNLKIVPPTTSKPDSDGFYTHICDVSGGTYNFTLAWSGQGI
                     AHIKGSTSNGWALNVPVNTMLEGERCSARVTIPPSSEGVKTLTISYAVLKSNFRATLT
                     VLVYFVRHPVITFKTGQRIAQVENQNRVSSKQYMHVAPYKTGNCVMVPLRFFVETCNG
                     SVSWDAKTRTAKVSMPGRKLGFSKNSANSTINGHQQKLASKCTIIAGRIMVPVSDLAD
                     MSGASFKLLKNGEMSFKYPA"
     gene            complement(7427..>8028)
                     /locus_tag="PK717_02045"
     CDS             complement(7427..>8028)
                     /locus_tag="PK717_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46481.1"
                     /translation="YLYFGTKLAFLPVKSTEQTPPTVAQFFRQRLRWGSGHMQVMRSI
                     RSYPWRDTAVGTKKDFDQRALSLWWNLTLTGPLEWVVYQLSTLVVLAMDVILVLNAFG
                     AGIPGAYFSQNPVLYWFLLCLNIPYTAFTFYCYIRYDEVFDKTFRPLSLGILVMDLAK
                     LLMASFVVFLLPAPYTFAAILSLFGKSPKIWVKTPRTEE"
BASE COUNT         2308 a         1983 c         1650 g         2087 t
ORIGIN      
        1 ggcaatactt catggcattt atgagaaggt tcccaaaaac ttcctcgatc gaaccagttt
       61 caaccttgat gcttctcccc ttgaggtatt catcaatatc aacaatgaaa ctcgctcccg
      121 tttttttctg catcgggtta agcgattctt caaaaagtat tttaagcttt gtctgaaggc
      181 caacgaaaag gccatgagca tcaaccagtt tgaaaacata tcttggcttt gtcactccaa
      241 aactcacaag atcatcaaca tggtgctgtt cacgcaatgc tgcctctctt atcatcttga
      301 ccacttccaa ggcgttcgga tttttctgca ccattccctc aagcagctcc gaaaatcccg
      361 aaataactgt aagcgggttc ctcaattcat gcgccacaga gccaagaaag gccctctttg
      421 cctcctcttt ttggaccgaa tctgtgatgt ctcttgcaat gccaatataa gaacctccaa
      481 tatgggagag cttgatttcg aaaactcttt ccaaattatc cgagtcaata agtttgacca
      541 taaattctgc gtccctgcca gaacgctgga tgcgcagggc cttccacaca gagataaact
      601 ctgttgccga tcgtccaagg aactctccaa cgctcctgcc aaggactttt acgcaatttg
      661 gtgaaacata ggacagaaaa ccatttctga cttcaaaaac gatgtctgat atattggcga
      721 tgatcaaatc cagcctgtct tgttcttcaa ggatttttcg cctgtccaca agacttgcgg
      781 ttatatcttt tataagtgaa atggtcccaa taatattgcc ttttggtccc ctgaaaggtg
      841 aacatcttgc attcagccat acttctttac ccaatattct aaaacagccg gcaaattctc
      901 cgggtgttcc tgttttggca acattcaaaa ccattgcctt aaattcatcc aacgatttcc
      961 cagccccaaa gatgtcctcg atatcactcc ccaaaaacag ccccccgtca ttaccggccc
     1021 aatctggttt cattttaaca aaaacacatt ttccattttc atccatcgcc aagacaatgt
     1081 cttcagtaga agccacaagc gcattaagaa actcattggt ttctaccaaa gcatccttgg
     1141 cttcaatgag tgaggtcaga tcagatactg acacataagc acacatcttc ccaccatagt
     1201 ttccaccgac aatatttgtc ctggtatctt ttattttatt gtcaccgcaa acaaacttca
     1261 tatcacttct tgacacagaa gtgtctccca aaaatgcacg tttaaccttg aaagcaaact
     1321 cggccctcaa ctctggtaca ataaggtcat aaaggttgac tttttcaact tcctgatccg
     1381 ataagccgat aaaatccaga aattccttat tataaaataa gaatttgtct ccatcgtgga
     1441 caaaaatggg cgtccctatg ccttccacaa gcttcctgtg gcgtttctcc gattcttcca
     1501 gtttcatctg ggaaagtttc tgggatgata tatcacggac aagggtgagg tatcttttac
     1561 cttcattcgt ctggatgaca tgtgaggaaa taagcacagg aatggcatcc ctgcttttgt
     1621 tatacaacaa tgtttcaaaa tttgagacaa taccatcctt tgcaagcatg gaaaggtatt
     1681 tcttcttttc tgcatcactg acccaaagtt caagctcttc aggagtcctc tctcctatct
     1741 ccgcttcaga gtatccaaaa agatctgtaa actctgctga aacatgaact atatttctgt
     1801 caggaaaaga caacaccaag tatggttcag gggcatgggt aaaaaaagtt ttgtactttt
     1861 caacctgttt agataattgg tcgagaagat tttttttctc gttgatattt ctcaccaaaa
     1921 ccaggacatg gttattcgat agcctagaga accttgcctc aaagtatttt ttcccatgac
     1981 cataatcaaa gtcgtactca taaattgttg ttcttcctgt aaaaaccgac ttgccaatag
     2041 tttgtaaaaa agcctccgca atgttggagg gcaatatttc cgaaacattc tttccaacat
     2101 gcctcctcca tggcatatcc agaaccattc catttggtat cttgcaatcg acaagctttc
     2161 cttcgctatc aatcagcagt aaagcgtctg ggaaagaacc aaaaatatcg cctatcgttg
     2221 gtttacaatt atcaacctcg caatcgggga gtgctaaaaa atcattcacg gcagcaggtt
     2281 tttcaacagg ggtaaaacaa accacaaaaa aatcgccaaa agaggagctt ctaaaaaaat
     2341 actgcgtgta atgattttct tctggtcttt ttaatctcac cgtaaaaaag gctggattca
     2401 taagtttcgg caagtccagg caatcatctg gatggataca acatggcctt gccccagaac
     2461 atatctcttg caagaatggt tctgaaacat acaatacctt ttcctcattg gcaataatta
     2521 gcccaaaatg tttctccatc ttcccgttta aaaattacaa ttatttttaa cactcacata
     2581 gcctcattga gtaaaattgg aaacaatttg tatacaatta cgtaacattt ttgcaagctt
     2641 agaaaacggc tataaaacgc tatttagaat cttttgcttg tctcatcgca tttaagatct
     2701 atatttttac aagctcaaat ccagtacaat aattaaccga aaaaattaaa atatttcaca
     2761 attgcacgaa aaacatccct caccattttt ttgctttata aacaaaaacg taatgtaata
     2821 tttttgggga tgagaaagca accaattttt tataacgcca acttcacaag atacgtaatg
     2881 agccagcttt ttgccttccc ttcagcctgg atatacggaa gtgccctgat gtgggtcaca
     2941 ctccaaatca caaattctcc actccttctc ggagtaatgg gcatgtgcag cagtctccct
     3001 tatcttgtcc tcacaccagt cacaggctcg atggcagaaa gatatgacag gcgaaaactg
     3061 acccttgtta caaacatcat aaatgttgtt caatcatcac tcctcgcctt tatggcgttt
     3121 acaggcctta tcaactaccc aatactggcc atctttgcac tcataaatgg tgctaccggt
     3181 gccattcagg aaccggcaag gcttgccttt ttgctcgaca tggtcgggcc agaacacctt
     3241 atacaggcaa acgctttgag ctcggtgact ttcaacatcg cgaggatact gggcccaatt
     3301 gttgccggtt tgctgctaac tgttcattcc ggatattgtt ttgccctgtc cgcgcttctt
     3361 ttggtgcctt caatcgtcat gtttacaata attgacgtca agagcacaaa aatagagcca
     3421 aggggttcga tgatcgtcca gttcgtggaa ggtgccaaat atatctccag acaaaaaata
     3481 ccattcctga tccttttcac gattgttcta gcatcaacat tcggctatgg cataaccatc
     3541 ctttcttcag caatcgccag tgatgtactg aagcttggct caataggcta cggtctattg
     3601 cagtcttccg ttggggtggg tgcactcatc ggggccctcg tcatatcaat gaccaaaagc
     3661 aggcaaggaa tcatggccaa attcagatct ggcagcatca tgcaggcagt gatgggactt
     3721 gcactcgctt tggtcctcct actttacccg acaatctggc tggccatccc gctttatgca
     3781 attttcggct atatgatggt gaaccagaac gtgctcgcat caagcatcct tccaatggtc
     3841 acacaggaag ggtatattgg aagggtaatg ggtttctact cctttgcagt ttcaggcgtg
     3901 atgcccattg gcaacctgat agccggagga ttgacccagg ttctcaccat accaattgga
     3961 cttttgattt cttatgttgc gctcctaatt ccaacactca taatatgtgt ttccccaaag
     4021 gcattgaaca aagacccatt ccaaaataaa atccagtcat gagaactttt ctggcttttg
     4081 ccatagcgac cacgatggcc cttggctggc catgcgcctt tccagacggc aaaggcaatc
     4141 cctacacacc caccgagttg ccaccaggcg agccacaggt gcattggtca atgacagagg
     4201 tcaaatcccc aacatcggcc tgcatcgtag aaaacaggct ctattttgca tcacaaaaca
     4261 tcgcaaactg ccttgatctt gaaaccggca aaaaagtttg gggggcagag cttgtctcaa
     4321 acattgtggc accgccatgc ttcgtgagtg gaagggcaat cttttgtgac aaggttggca
     4381 atgtctattc gatagactca tcaaatggca aggtaatcga tagagcctca acaagctccg
     4441 gcatagtcgc accgccaata gccatctccg gattaattta tgtttcttca acttcaggca
     4501 aagttgtttc cttcacgcct gacctgaaag agcagatgtc agtcgacctt gaaacgccgc
     4561 tctcatgctc ctccatcatc acctcccagg gacttttcca gcaaaccagc gatggcacca
     4621 tatatgttat aaatccagaa aatggagcaa ttctttccaa catcaaggca gacccgccaa
     4681 tagggcagat atgccaaaat gatggtttaa taaatattcc agtgcaatca ggggtcacaa
     4741 ggctaaaagg agaccaaaga ataccgattg aattgccatc ttcaccatcc tgcatggttg
     4801 ctgttcccgg aggacctctt gtcgttggaa caagctttgg tctttatgga atcagcggcg
     4861 acaagctctc gtggtctatg aaaacggaaa aaccggtttc agccgtatcg gcaaactccg
     4921 agatcgccgt gtttggatgt gccgggggac ttgtggccgg agtgaggcta aaggacgggg
     4981 aagtgctctg gaaagcaacc atggccggag acatagtgca cccaatcccg ataatccgtg
     5041 atggccttat ggtgtcttcg tcaaaatcag tcaccttttt caagctctgg gacttgaatc
     5101 cggaaccatc aataattgac cttggccatg ttccaacagg cgagattgcc acaggattat
     5161 tttcaatgtc aaatccggca aattccaccg gccctgtaca agtgatatgt acatcagaaa
     5221 aaccagagat aacaatcaat cctggcaatg ccataattgt gccaggagga aaggccttct
     5281 tcaacgttgt actcgattcc acgggaatca aggaaggtaa atattcaacc acaatcacca
     5341 tcaagacaaa aaccagctcc tacaaggttg ttgtgggtta caatatcgtc ccgcaaccct
     5401 ttgttgccga gttgaagctc ggcgactcga tcatgaagat gaagaggggc acccaaaaat
     5461 gggaagtaaa gcttgacagt ccaccgttcc tccaatcgaa taggacaatg gtcccgctcc
     5521 gcgcaatatc agagtcattc gggccaaagg tcacttatgt caaggatggt tgcccagggt
     5581 ccggaagagt ggacataacc cttggcggaa caatcattta ccactgcatc ggcacaaaca
     5641 ttcttacatt gaagctccca ggcaaacagc cggaggcaaa aacatttgac acagcgtctg
     5701 ttattgttgc cggacgcact tttgtaccca taaggttcat tgccgaagca tttttagcca
     5761 cagtaaactg ggacgcaaac acaaaaactg ttataataac ctaccagcca ccatgaaaaa
     5821 actaaccatc tttgtcttgg tcttgctttg tgccctatcg ggggtgacct ctgctgtcgc
     5881 tttgatccac gaaagcgctg gtgcaacata tacatttatt ggtgggtcat ggactgacaa
     5941 aaactcctgg gaaccggcga ccgcagggaa tggccccgga cttgatgaca acgtagttat
     6001 tccagagggc ggaaatgccg taatgccaaa gtcttgcgac atcggaacac tcactgtcag
     6061 gggaaaactc acaatgggca acgacctcag attaaactcg atgaccgtgg aaactggcgg
     6121 cgtttttgag ctcggggccg atctgacctg cacaggtatg ctgaaaaaca acggccagat
     6181 aaaaggtgga gacgttttcc taaaatgcca gaactttgaa aataatgctt tcttcattgc
     6241 aacaggaaaa acccagatag ttgctgtttt caacataaaa aatctcggca cattgaaagc
     6301 aggcccactt ttgatgctga gatcaaacaa tttcacaaat gacgggcttt tggaaacaac
     6361 tgcaaagcca ggcacagtgg ctgttttcat caacaataat cttgcaaaca acggtagtat
     6421 cagggcttca gccggaaaaa gcggaagcaa cggcggggca atttttgttg cctgctcctc
     6481 atttgaaggc accggaaaag tttacccggg cgacggcggg aacggcaatc cgcccggaaa
     6541 aaagggagtt gccctcattg gttcttcctt tgaccagacg ggctggacaa attgggaacc
     6601 gactgcccca aagggcaatc cgcctcagaa tctcacagcc tggaacgcac aggtcgttca
     6661 ggcaaaagat ggcaaatcat caatatccac cggcaacctc aagatagttc caccaaccac
     6721 atcaaaacct gacagtgacg gcttttatac ccatatctgt gacgtttccg gtggcactta
     6781 taattttaca cttgcatggt caggtcaggg cattgcccac atcaagggtt cgacatcaaa
     6841 cggctgggca ctgaatgttc ccgtaaacac aatgctggag ggtgaaaggt gctctgccag
     6901 ggtcaccata ccaccatcct cggagggcgt taaaaccctg acaatcagct acgccgttct
     6961 gaagtccaat ttcagggcca cactgactgt tctggtatac tttgttaggc accccgtgat
     7021 aaccttcaaa acagggcaaa gaatcgccca ggttgaaaac cagaacagag tctcctcaaa
     7081 acaatacatg cacgttgccc cgtataaaac cggcaactgt gtcatggtgc ccctcagatt
     7141 tttcgtggag acatgcaacg gctcggtctc ttgggatgca aaaaccagga ctgcaaaggt
     7201 ttccatgccg ggaaggaaac ttggtttctc aaaaaactct gcgaattcca ccattaatgg
     7261 acaccagcaa aagctggcct caaaatgcac catcatagct ggaaggataa tggttccggt
     7321 ctcggatctg gctgacatgt ccggggcatc attcaaactg ctcaagaatg gtgagatgtc
     7381 cttcaagtat ccggcataga atagttagct tatctcaaag agacatttat tcttctgttc
     7441 taggcgtttt cacccagatt ttgggcgact tgccaaaaag cgacaggatg gctgcaaaag
     7501 tgtacggcgc aggcaacaag aaaacgacaa agctggccat cagcagtttc gcaaggtcca
     7561 tcaccagaat acccagggat agcggcctga atgtcttgtc aaaaacctca tcatacctga
     7621 tatagcagta gaaagtgaaa gctgtatacg ggatgttcag gcaaagcaaa aaccagtaca
     7681 gcaccgggtt ttgtgaaaaa tatgcccccg ggatgccggc tccaaaagca tttaatacaa
     7741 ggatgacatc catggccaat accaccagtg ttgaaagctg atacaccacc cattcaagcg
     7801 ggccagtcaa tgtgaggttc caccacaagc tcaatgccct ctggtcaaaa tccttttttg
     7861 ttccaacagc ggtatcacgc catggatagg agcggattga ccgcatgacc tgcatgtgcc
     7921 cggaacccca tctgagcctc tgcctgaaaa attgtgcaac agttggtggt gtttgctctg
     7981 tcgattttac tggcaaaaag gcaagttttg ttccaaaata caggtaag
//
LOCUS       DAOSRI010000040         7914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_8633_length_7914_cov_3.989184, whole genome shotgun sequence.
ACCESSION   DAOSRI010000040 DAOSRI010000000
VERSION     DAOSRI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_7914_cov_3.989184"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK717_02050"
     CDS             <1..490
                     /locus_tag="PK717_02050"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOR46482.1"
                     /translation="DIIVFPSDQTKKGIAPGSITVKDVYQILPIDDTWVKGKISGAKI
                     KEICESSVSGERGVLQLWGLSFVFTRSKDPGNRVSEAYIIKNDEKTPVDTSKTYSIAM
                     PSREYHTLPGFGHEVLRNVTTDVNSSCIYLRKTIFEYIKKLTEAGQPVNQAKRKFGFV
                     EP"
     gene            complement(573..1286)
                     /gene="rnc"
                     /locus_tag="PK717_02055"
     CDS             complement(573..1286)
                     /gene="rnc"
                     /locus_tag="PK717_02055"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOR46483.1"
                     /translation="MLNLFQKFRLRQDSNLTAQQKALFKRLGLQNSYKQIFLEALTHP
                     SYTSENDSPSYQRLEYLGDAILGSYVAYRLYMRHRDWQEDKLTRARASIVSQPALAKS
                     ARKMGIPHCLLLGKGERANGGSERDSILCDCFESIVAVVFIAKGFQKTCDFLDALGII
                     SMATDKEDPKSELQSLSQKEGLGLPHYHIVGSDGPAHCMRFEAKVSVGENIIATAWGN
                     SKQEAEQNAAIEALKKIEK"
     gene            complement(1246..2232)
                     /gene="plsX"
                     /locus_tag="PK717_02060"
     CDS             complement(1246..2232)
                     /gene="plsX"
                     /locus_tag="PK717_02060"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOR46484.1"
                     /translation="MIRIALDAMGGDHAPKEIISGAILARSSMELDITLIGDKTAIEE
                     ELKIQKSPFMFPVVHTTEVVTMNDHPSEVLRTKKDASIFVATRLVAEGRCDAIVSAGS
                     TGAQLAAAIFILGRVRGIERPGVATPVPQVNGKLAMMCDGGAVSDCKPNNLLEFAQMS
                     SAYCQNVMGIDNPRVALLNIGEEPEKGSELTQATHKLLLKSGLNFIGNIEGKDVLKGN
                     ADAIITDGFTGNVILKAIEGIASDVMGLVKKAAKTSLSSKMGGLLLLPAIGNIRKMMD
                     YSEYGGAPLLGVNGISIICHGRSNGKAISSAIRQAVNCCNAQLISKISSQTR"
     gene            complement(2229..2441)
                     /gene="rpmF"
                     /locus_tag="PK717_02065"
     CDS             complement(2229..2441)
                     /gene="rpmF"
                     /locus_tag="PK717_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOR46485.1"
                     /translation="MPEPKKKVTPRRSRLRRAHQKIDVPALVECKNCGKMTRAHHVCT
                     YCGFYNEELVVVPKVKKVKTSKEEQD"
     gene            2567..2830
                     /gene="rpsT"
                     /locus_tag="PK717_02070"
     CDS             2567..2830
                     /gene="rpsT"
                     /locus_tag="PK717_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOR46486.1"
                     /translation="MLRHKAQQKSVRQSAKRHERNVTQKSAAKTAIKKAIASIGTGEA
                     TTNVQIAIKSLDKLESHGIMHKNAVARKKSRLMAKLNKSLQQA"
     gene            complement(2955..3944)
                     /gene="holA"
                     /locus_tag="PK717_02075"
     CDS             complement(2955..3944)
                     /gene="holA"
                     /locus_tag="PK717_02075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR46487.1"
                     /translation="MNTFTDELRKIRKGEQPKSLYWVCCSNPYLEREFLELIASSVGG
                     GAMSKVSIDLTETEINDFVFTVTSGSLLSGGKVIIVSGALKIKGDDGKRITDAITNAN
                     PANIVIFTEPDIPKTTSLGKHLASKAVQITESGLTEKMLSAWVKKKFQEKNCQVADDA
                     SSIMVERSMGDLVALSGEIEKIASWLGEGATATRKDIEQLVPENPEIIVFQVFDALGA
                     KQTSKGIKLLHGLVRNGEPAERVMAMLYNHFMRVLVAKEMSSQKATEKQIAERLKCHP
                     FAAKKALESATQFKKDELISYLSAIANADVAAKTGMADALSAIESALFQLAGN"
     gene            complement(3941..5227)
                     /gene="asnS"
                     /locus_tag="PK717_02080"
     CDS             complement(3941..5227)
                     /gene="asnS"
                     /locus_tag="PK717_02080"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOR46488.1"
                     /translation="MVTISELGKHVGEDVKISGWLHNSRSSGKLVFLVIRDGTGFCQC
                     VAFQGNLSPEQFKLAGDLTQETSFFVVGTVKENPKQKGVFEIDVKQIEKYGDSVDFPI
                     SPKEHGVGFLMSNRHLWLRSQKQWATLRIRARIVKSIRDFLDSEGFVNVDAPCITPSS
                     CEGTTTLFEIDYFGKPAFLSQSGQLYNEATAAAFGKVYCLGPVFRAEKSKTRRHLIEF
                     WQVEPEAAFVLHEENMKIQERLVERIVKDALHDCETELKILERDTTVLQHVITPFPRI
                     TYTEAVKICQQEGSSIEWGGDFGAPDESIVSSKFDRPVMIEKYPAQVKAFYMKQDPED
                     PRVVLCDDMIAPEDVGEIIGGSQREDNLDVLLKKIADHNLPREMFDWYLDLRKYGTFP
                     HSGFGLGIERATSWIAGSQHIREASPFPRLLDHNYP"
     gene            5368..5613
                     /gene="secG"
                     /locus_tag="PK717_02085"
     CDS             5368..5613
                     /gene="secG"
                     /locus_tag="PK717_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOR46489.1"
                     /translation="MVLFRNIMVTVVIIAGIGMVFGYIVSSPKASGLGAIGGTAQKFK
                     VRKSRDLFLDKLILWSALIFGVCAFVLSIVDPSIWTM"
     gene            5621..5705
                     /locus_tag="PK717_02090"
     tRNA            5621..5705
                     /locus_tag="PK717_02090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5655..5657,aa:Leu,seq:gag)
     gene            5875..5949
                     /locus_tag="PK717_02095"
     tRNA            5875..5949
                     /locus_tag="PK717_02095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5907..5909,aa:Val,seq:tac)
     gene            6046..6489
                     /locus_tag="PK717_02100"
     CDS             6046..6489
                     /locus_tag="PK717_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR46490.1"
                     /translation="MKNQNKVVAYITRGTKLLVFDHANFPEVGTQVPAGTIEDGETPT
                     FAVMREVFEETGLNKLYLRDFLGMQTLVLEKPEGKMLINRHYYHFEVPDDVPDTWAHY
                     ETKPSDGSFDLILFNFRWVDFSKDNITLAGKQDFFLRLLGNKLKK"
     gene            6600..6675
                     /locus_tag="PK717_02105"
     tRNA            6600..6675
                     /locus_tag="PK717_02105"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6633..6635,aa:Asp,seq:gtc)
     gene            6805..>7914
                     /locus_tag="PK717_02110"
     CDS             6805..>7914
                     /locus_tag="PK717_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR46491.1"
                     /translation="MRRHLSLVLVATIILMVQPAYAAENKPDISLDMLVVLDGNTVAD
                     FLPQNYVQTDSISLDSSLAFAGRETMKSVESFISSNCQGIVEQVYQKYSILTKGFACK
                     IKTRNIKRLASIPGIKSVEKIPDYKLDLDTSTTVIGANDAWKMLDQNRQPLNGTGVII
                     GVIDTGLDWRHPDLGSRMGSKEKVVDGYDFADGADISNDTEQHGTHVAGICAANGKVK
                     GVAPGANLAGYKVFTSEKGRSVNVGANINAALEKAITSKCGVVNLSLGSPGGSTGGEE
                     GNSIYHKVVKAGIVVVASAGNNGSRCLTQPYVVGTPSTYMPVISVAASDDMKHPEISV
                     TNPPGENMRILGREFDLPPKWPDSEWEVVDCGYGYP"
BASE COUNT         2097 a         1746 c         1742 g         2329 t
ORIGIN      
        1 tgatataatc gttttcccat cagatcagac caagaaaggt attgcaccag gaagcataac
       61 agtcaaagac gtctatcaga ttctacctat tgatgacact tgggtgaaag gcaagatttc
      121 tggagccaag ataaaagaaa tttgtgagtc atcagtctct ggtgaaagag gtgttctcca
      181 gctctggggt ctttcatttg tcttcacaag gtcaaaagac ccaggtaacc gtgtctcaga
      241 agcctatatc atcaagaatg atgagaaaac cccggttgac acttccaaga catattcaat
      301 tgccatgcca tccagagagt atcacacact cccaggtttt ggccacgaag ttctaaggaa
      361 tgttactact gatgttaatt cctcttgcat ttaccttagg aaaacaatat tcgagtacat
      421 caagaaactc actgaggcag gacagccagt taaccaggca aagaggaaat ttggttttgt
      481 agagccgtaa tcaggaaaga ctaaagtaaa ttaaaaaagg cacccttttg gggtgccttt
      541 tttaatttgt agagttgaga aatgtaaaac tattattttt cgattttttt gagtgcttct
      601 attgcagcgt tttgttcagc ttcctgtttt gaatttcccc aggcagtagc tatgatattt
      661 tccccaacag agacttttgc ttcaaatctc atgcagtggg caggtccatc gctcccaaca
      721 atatggtaat gtggaagccc gagaccttct ttctgagata acgattgcaa ttcgcttttt
      781 gggtcttcct tatctgtcgc catagagata atccccaaag catcaagaaa atcacatgtt
      841 ttttgaaagc cttttgcaat aaacacaaca gccacaatag actcaaaaca atcgcagaga
      901 attgagtctc tttcagaacc tccgtttgct ctttcaccct ttcccaaaag aaggcagtgc
      961 gggatcccca tttttcttgc tgattttgcg agggctggtt gtgagactat tgaggccctt
     1021 gctcttgtga gtttatcttc ttgccaatct ctgtgcctca tatacaagcg ataggccaca
     1081 taactgccca ggattgcgtc tccaaggtat tccagtctct gatatgaggg agaatcgttc
     1141 tcgcttgtgt atgacgggtg ggtcaaggct tccagaaaga tttgcttata gctattttgg
     1201 agcccaagac gcttgaaaag agctttttgc tgggctgtta gattactatc ttgtctgaga
     1261 cgaaattttt gaaataagtt gagcattgca acagtttaca gcttgtctaa ttgcggagga
     1321 gatggccttg ccattcgaac ggccatggca aattattgat atgccattca cacccaaaag
     1381 aggcgccccg ccatactctg aataatccat catttttctg atattaccaa ttgctggtag
     1441 cagaagcaat ccacccattt ttgagctgag ggacgtcttg gcggcttttt ttaccagacc
     1501 cataacgtcg cttgcgatac cttctatcgc cttgagtatc acatttccag taaaaccatc
     1561 tgttataatt gcgtctgcat tccccttaag aacgtctttt ccctctatgt tcccaataaa
     1621 attaagccct gattttaaca aaagtttgtg tgtggcctgt gtaagttctg agcctttttc
     1681 tggctcttca ccaatattaa gaagcgccac ccttgggttg tctatcccca ttacgttctg
     1741 gcaataagca gatgacatct gggcaaattc aagaaggttg tttggtttgc agtctgatac
     1801 agctccacca tcacacatca ttgccaattt gccgtttacc tgtggaactg gcgttgccac
     1861 tcctggtctt tctatgcccc ttacgcgacc aaggataaat attgcggcgg ccagttgtgc
     1921 cccagttgag ccagcagaca cgattgcatc gcacctgccc tcggccacca gtcttgttgc
     1981 tacaaaaatt gaagcatcct tcttggtcct gaggacctct gaaggatggt cattcatggt
     2041 gacaacttct gtcgtgtgaa ctactggaaa cataaaaggc gatttttgga ttttaagttc
     2101 ttcctcaatc gccgttttgt cgccaataag ggtgatatca agttccatgg aggagcgggc
     2161 aagtattgcc ccagaaatga tttccttggg ggcgtggtcg ccccccatgg catcaagtgc
     2221 tatccttatc aatcctgctc ttccttggaa gtttttactt ttttcacttt cggtactaca
     2281 acaagctctt cattgtagaa accacaatag gtgcaaacat gatgtgcacg ggtcattttt
     2341 ccgcaatttt tgcactcgac cagtgctgga acgtcaattt tttggtgagc cctgcgaagt
     2401 ctgcttctgc ggggtgttac tttctttttt ggttcaggca tcttaatcct ccgagtattg
     2461 ttggttacgc aaacggcaag tatattatct aaccacgaca tgtcaaccct tgacaggtga
     2521 gaactctttt gtacaatcgc taaggttttg aaatattgga ggatacatgc tgagacataa
     2581 agcacaacag aaatcagtca ggcaatcggc caaaaggcac gaaagaaacg tgacccagaa
     2641 atcagccgca aagacagcca taaagaaagc cattgcttca atcgggaccg gtgaagcaac
     2701 aacaaatgta cagatcgcca ttaagtcgct agacaaactc gaatcccatg gcatcatgca
     2761 caagaatgcc gttgccagaa agaaatccag acttatggca aagctgaaca aatctttgca
     2821 acaagcctaa gcaaaaacta ttgtcaatta gaggaccttt cccaggtagg gaaggtcctt
     2881 ttttatttta catggtttta ctactggagc catgctgttt tatgcaattg tttggtcaat
     2941 gaggtctata ttggctagtt tcctgcaagc tggaaaagcg cagattcgat tgcagaaagg
     3001 gcatccgcca ttcctgtctt tgcggcaaca tctgcattgg caatggccga aagataagag
     3061 atcaattcat cttttttgaa ctgtgttgcc gattcaaggg cttttttagc agcaaatggg
     3121 tggcacttta atctttcggc gatctgtttt tctgttgcct tctgggacga catttctttt
     3181 gccacaagta ctctcatgaa gtgattgtac agcattgcca tgactctttc ggcgggttca
     3241 ccgttccgaa cgagtccgtg gagcaatttg attcccttcg atgtctgttt tgcgccgagt
     3301 gcatcaaaga cctgaaatac aatgatttct ggattttcgg ggaccaactg ttcgatgtcc
     3361 ttgcgcgttg ctgtagcgcc ttcgccaagc caagatgcta tcttttcgat ttccccggag
     3421 agtgccacaa ggtcacccat ggagcgttct accattattg atgaagcatc gtcagccacc
     3481 tgacagtttt tttcctggaa cttctttttt acccatgccg agagcatctt ctcagttagg
     3541 ccagattctg ttatctggac agcttttgaa gctaggtgtt ttcccagcga tgtggttttt
     3601 ggtatatctg gctcagtgaa aataacaata ttggcaggat ttgcatttgt tattgcatca
     3661 gtgattcttt ttccgtcgtc gcctttgatc tttaatgcgc cagaaacaat tatcaccttg
     3721 ccgcctgaaa gaagacttcc ggaggtaact gtaaatacga aatcatttat ctcggtttcg
     3781 gtgaggtcaa tgctgacttt tgacatcgcc ccaccaccaa cagatgaagc aatgagctcc
     3841 agaaattcac gctcaagata tggattggaa cagcaaaccc aatagaggga ttttggctgt
     3901 tccccttttc tgatctttcg gagctcgtcg gtaaaagtgt tcacggataa ttgtggtcaa
     3961 gaagtcttgg gaaaggtgat gcttccctga tgtgttgtga gccggcaatc caggaagttg
     4021 ccctctctat tccaagtcca aagccggaat gtgggaaggt gccgtatttt ctgaggtcga
     4081 gataccagtc gaacatctcc cgtgggagat tgtggtctgc aatctttttg agcaagacat
     4141 cgagattgtc ttccctctgg gagccgccaa ttatttcacc aacatcctcc ggggctatca
     4201 tgtcgtcaca gagaacaact cttgggtctt caggatcttg tttcatatag aaggctttaa
     4261 cctgggcagg atatttctcg atcattaccg gcctgtcgaa ttttgatgat acgatactct
     4321 cgtctggtgc cccgaagtct ccgccccatt ctatgctgga accttcctgc tggcagattt
     4381 ttaccgcttc tgtataggtt attcttggaa acggagtgat aacatgctgc aagactgtcg
     4441 tgtccctttc gagtatttta agctctgttt cgcaatcatg aagagcatcc tttacaatcc
     4501 tctccacgag cctttcttgt atcttcatgt tctcttcgtg gagcacaaat gcagcctctg
     4561 gctcgacctg ccagaattcg ataaggtgtc ttcttgtttt gcttttttct gccctgaata
     4621 ccggtccgag gcaatagact tttccaaacg ccgccgctgt agcttcgttg taaagctggc
     4681 cagattggct caaaaaggct ggtttgccaa aatagtctat ctcaaagagt gttgtggttc
     4741 cttcacatga tgatggagtt atgcatggtg catcaacgtt cacaaaacct tctgaatcga
     4801 gaaaatccct tatggatttt acgattcttg ccctgatgcg aagcgttgcc cactgttttt
     4861 gggaacggag ccacaggtgc ctattggaca tgagaaatcc cacaccatgc tcttttggtg
     4921 aaattgggaa atcaacagaa tctccatatt tctcaatttg tttgacatca atttcaaaaa
     4981 cacccttttg tttcgggttt tctttgactg taccaacaac aaagaacgaa gtctcctgtg
     5041 tcaggtctcc tgccagcttg aactgctctg gcgagaggtt accctggaaa gcgacacact
     5101 ggcagaagcc agttccatcc cttattacaa gaaacaccag cttgccggac gatctcgagt
     5161 tgtgcagcca gcctgaaatt ttaacatctt cgcctacatg ttttccgagt tcagatatag
     5221 taaccatgtt cctccttaag ctataaaaag atactatttg ttgtgaaaaa cgcaaggtaa
     5281 ttttaagttc ttaagattac ccccttgaaa tatgttggcc aaatggatat ataaacctaa
     5341 gcttcaagat tgattggagg atgaaacgtg gtattgttca ggaacataat ggtaactgtt
     5401 gtaataatcg ccggaatcgg aatggtgttc ggctacatag tttcatcacc aaaagcatct
     5461 ggtcttggtg ccattggtgg cactgctcag aagtttaagg tgaggaaatc acgcgactta
     5521 tttctggaca agctgattct ctggagtgcc cttatatttg gggtttgcgc cttcgttctt
     5581 tctattgtcg atccatcaat ctggactatg tagtttgttt gccgaggtgg cggaattggt
     5641 agacgcgtac gtttgagggg cgtatgggga gacctgtgcg ggttcaagtc ccgccctcgg
     5701 caccacttaa aaaccaaaag gccggctttt gccggccttt ttcatttttg gtaaaatgtg
     5761 ttttgtcatc ctggcacaag aaaagagttc ttcaaaccgg tagtttttgt taccaatggt
     5821 caaacgtaag taatcttgac gtgaaagaag tagacgctaa aatatttttg ttctgggcgg
     5881 atagttcagc ggtagaacgc ttgccttaca cgcaagaagt cacaggttcg aatcctgttt
     5941 cgcccaccat ttttcgatcc tcttaactct aatacccaac aatctttatc ttaaatgtat
     6001 aatcatttgc taacagtatt gccctagcat ttttttgaaa tgacaatgaa aaaccagaat
     6061 aaggtagttg catacatcac aagaggtaca aaacttctcg tttttgatca tgccaatttt
     6121 ccagaagttg ggacacaagt gccggcaggg acgattgaag atggtgaaac accgactttt
     6181 gcagtgatga gggaagtctt tgaggaaact ggtctgaaca agctctacct tagggatttt
     6241 cttggtatgc aaacactggt gttggaaaag cctgaaggaa agatgttaat caacaggcat
     6301 tattatcatt ttgaagtccc ggatgatgtt ccggatacat gggcccatta tgaaacaaag
     6361 ccaagtgatg gctcattcga cctgatcctt ttcaatttca ggtgggttga tttttcaaaa
     6421 gacaatataa ccttggcagg caagcaggat tttttcttgc gtcttttagg caataaatta
     6481 aaaaagtaaa acaataaaat attgtattaa tgattaattt gttactcttc tccaggtcag
     6541 cttagcgttc ttaattgaca aactgtttgg gccttgctta taatacgtgg acgtttcgcg
     6601 gggtcgtggt gtagcggtca acacgccagc ctgtcacgct ggagaccggg ggttcgaatc
     6661 ccctcgatcc cgccaaatag ccaactcaga aaaatcaaat ctcccaaaaa atcagattaa
     6721 aactgggcat tcagaccttt ccaaatttgt tgactggatt gcatttcaac ctataattcc
     6781 caaccaaatg actttggagg tccaatgaga cgccatctca gtcttgttct tgtagcgacc
     6841 attattttga tggtacaacc tgcttatgca gctgaaaaca aacctgatat ttctcttgac
     6901 atgctggtag ttctggatgg caataccgtt gcggatttct tgccacaaaa ttatgtccag
     6961 acagactcaa tctccctgga ttcatcgctt gcctttgctg gacgcgaaac aatgaaatcg
     7021 gttgaatctt ttatatcttc aaattgccag ggcattgtcg aacaagttta tcaaaaatac
     7081 agcattctta caaaaggttt tgcttgcaag ataaaaacac gaaatattaa aagactggcc
     7141 agtattcctg ggatcaagag cgttgaaaag attccggatt acaagctcga tcttgatact
     7201 tcaacaaccg tcataggtgc caatgacgct tggaagatgc ttgatcaaaa tcgccaaccg
     7261 ctgaatggaa caggagtcat cattggtgtt atagatactg gccttgactg gcgccatccc
     7321 gaccttggtt caagaatggg ttcgaaggaa aaagttgttg atggctatga ttttgccgat
     7381 ggcgctgata tatccaatga tacagaacag catggtactc atgttgctgg catctgtgca
     7441 gcaaatggga aagtcaaagg cgttgctcct ggagcaaatc tcgcaggata taaagttttc
     7501 accagtgaaa agggtagatc agtcaatgtt ggtgcaaaca tcaatgctgc acttgagaaa
     7561 gcaatcacat cgaagtgcgg ggtagtcaat ctttcgctag gttctccagg ggggagcact
     7621 ggtggcgaag aaggaaattc aatctatcac aaggttgtaa aggccggcat tgttgttgtc
     7681 gcatctgcag gcaacaatgg ttcacgctgc cttacccagc cttatgttgt aggaacgcca
     7741 agcacttaca tgccagttat ttctgtagcc gcatctgacg atatgaagca cccagagatt
     7801 tccgtaacaa acccaccagg tgaaaatatg cgcattctgg gaagggagtt tgaccttccc
     7861 ccaaaatggc cagatagtga atgggaggtt gttgattgcg gatacggtta tcct
//
LOCUS       DAOSRI010000041         7704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_8927_length_7704_cov_4.414564, whole genome shotgun sequence.
ACCESSION   DAOSRI010000041 DAOSRI010000000
VERSION     DAOSRI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_7704_cov_4.414564"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK717_02115"
     CDS             <1..494
                     /locus_tag="PK717_02115"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOR46492.1"
                     /translation="PGRDDIRLLIKTIRGSFKNAILRSSIIVGFPGEGRQDFDELASF
                     VRETMFDRLGVFKYSPEENTLSYKMKQISSRVASYRWNTLMALQSEISLENNSRLKGQ
                     MLPVIIDGIGKDTAIGRSMADAPEIDGTVSVKGANIKRGDVVNVKVNSFSPYDLGGVK
                     VGH"
     gene            484..1026
                     /gene="thpR"
                     /locus_tag="PK717_02120"
     CDS             484..1026
                     /gene="thpR"
                     /locus_tag="PK717_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOR46493.1"
                     /translation="MAIRTFIALGLDASILEYIDKIMPVLKSSGASASWTRKGNFHLT
                     LKFLGDTEESIAQKISVELEGMVFQKIPYRLSRFGGFPNLRKPRVLWVGLESPMCIEV
                     AQKIDAVCNKFGFPLEERMFVPHLTLGRIRESFDFVKTVVELPPPPQGELFFKNVVFF
                     KSILSNSGPTYEPLWSKELG"
     gene            1023..2063
                     /gene="recA"
                     /locus_tag="PK717_02125"
     CDS             1023..2063
                     /gene="recA"
                     /locus_tag="PK717_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOR46494.1"
                     /translation="MSEKIPEKTERDKALDSAIASIEKQFGKGTVMKLGDGHGRMAVE
                     AIPTGILSMDIALGVGGFPRGRIIEIYGAEGSGKTTIALTTIASVQKMGGKAVFIDAE
                     HALDPSYAERLGVNLQDIIISQPDSGEQALEIAEILTRSGAIDLFVVDSVAALVPRAE
                     LEGEMGDSVVGLQARLMSQAMRKLAGVMGKSRTCGIFINQLREKIGVMFGNPETTTGG
                     RALKFYSSVRVEVRKGESQKDGDRIVGTMVKCKVVKNKVAPPYLSGDFEIVFGQGVSR
                     ESQLLDMGVNLNIIEKSGTWFSFDGERLGQGRDNAKMTISQKPEMMAEIEKRIRSAYG
                     MAPQGQPHSEEK"
     gene            2020..2481
                     /locus_tag="PK717_02130"
     CDS             2020..2481
                     /locus_tag="PK717_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOR46495.1"
                     /translation="MEWPRRDSPIAKKNNSKAFTYSLRLLAARDRAELEIREKLAKKG
                     FLEKEAEEAVKKLRKLGLLDEKKFIKEYVRVKRARHWGPLKVKLGLVALGIQKSDADA
                     EIRQTDWREPLKKLVKSNKGLEKRKLYSKLSRLGFPSDLIKSEMKCEEQDD"
     gene            2474..4018
                     /gene="rny"
                     /locus_tag="PK717_02135"
     CDS             2474..4018
                     /gene="rny"
                     /locus_tag="PK717_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOR46496.1"
                     /translation="MIETIVGLLGLALGAAVAFFYAMNASAKSADGKIRRAEEECKRI
                     VSQAKDEAETQKKEILVNAKDEAMKLVAEREKEFRQQRQELVDIERRIAQREEKIEQR
                     SEAVGKRENTITTREKELDEKHEQADQIVDKQRKELERIAGLNQETAKQIILDELRQK
                     MSYELAQKAKNMEEEAKDKALQKAREVILGSVQRIASEYTAEVTVSAVPLPSEEIKGK
                     IIGREGRNIRTFETATGVDLLVDDTPEVVTISCFDPVRREIGKLALEKLITDGRIHPG
                     RIEEVVEQATREFEDRLKLEGDQALLEVGLQQAKPELVKLLGRLRFRTSYGQNVLAHS
                     IEVAMLSAHMANELGLDARKAKRAGIFHDIGKAVDREREGSHDELGAEILRKNGEPIE
                     VIEAARLHHSDIAPTSVYAVLVQAADAISASRPGARRESFEAYIQRLESLETIAKSFR
                     GVEKTYAIQAGREIRVIVKPKDIDDVLAYQMARDIAARIENELTYPGTIKVTVIREVR
                     YTENAK"
     gene            4015..4794
                     /locus_tag="PK717_02140"
     CDS             4015..4794
                     /locus_tag="PK717_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOR46497.1"
                     /translation="MIVLFIGDIVGKPGRKAIEAVVPGLVKQYGVDFVVANGENAAGG
                     FGLTPDISNKLRAAGIHGITLGNHLYDQKEADELLENERLLARPANFPPGNPGSGHFV
                     LEGLAGTLAVVSLQGRVFMTPIDCPFRVADKIVEELRSTTKAILIDFHAEATSEKQAL
                     GIYLDGRVSAVLGTHTHVQTADERILKGGTGFITDVGMTGPHESVIGMEPSAPISKMI
                     SARRARFEVAEGDVRFCAVLLDIDRETGQCKKIERMMINVP"
     gene            complement(4859..5548)
                     /locus_tag="PK717_02145"
     CDS             complement(4859..5548)
                     /locus_tag="PK717_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46498.1"
                     /translation="MNEGTFASEIINRSLLYGILGYLPEEICNVLVLDGPKQTQMALI
                     LENAGYKVARICPQKDDDPTCIASTTEGIDGESFGVVICPGWLSDNEDPEKISREAMR
                     LLVQGGFFFGSVPGRFAAAVDCVPQSTSNASSIANGIDETQWWGTKELFTPNDIACEL
                     EAIGFEVVDLYGWEHALTKLPGEKLSATDWDEGFFEDFLELEFRLAQERSILGMAPTI
                     QFVAKKPNPEP"
     gene            complement(5562..5756)
                     /locus_tag="PK717_02150"
     CDS             complement(5562..5756)
                     /locus_tag="PK717_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46499.1"
                     /translation="MSCKKTNSTKCNCTHEPCERKYRCCECLSFHKSQNELPACYFTV
                     EEEKTYDRSISFFCKNHVKI"
     gene            complement(5817..7034)
                     /gene="mazG"
                     /locus_tag="PK717_02155"
     CDS             complement(5817..7034)
                     /gene="mazG"
                     /locus_tag="PK717_02155"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOR46500.1"
                     /translation="MNFSWIIEKELMISSAPENIQMLSRLRDTGIKAVVSLSQLPFTD
                     KDFEDLGLFNLKLQIPEDKPPTLEQLREFIFWAGFMKKHGLPLLIHCQEGYERSATLA
                     ACYLVIFENMTANQAIEEVRAIRGDNSVAFLHNQGFVNECEYVKPLLTNTSDISFYEA
                     KLLIDLLRRHCPWDREQTPNSMMQNLLEETYEVWEAVKQKDMAHIKEELGDILLQVLM
                     VSRMLSEEQSFSVEDVIATMQEKLVYRHPHVFGESKTKTVDSVLDQWNSLKKQNKNNG
                     SIRDIPSNLPPLGRAERIMAHAKGLGFDWDKTEGVLEKLDEEIQELKEAIQTKDKGEV
                     ENELGDVFFVLLNLARFLDVGPEVALSRTLDKFIKRFEKIENHSNETGKPIGQMTLGE
                     MDEIWNKAKKDHQ"
     gene            complement(7037..>7704)
                     /locus_tag="PK717_02160"
     CDS             complement(7037..>7704)
                     /locus_tag="PK717_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOR46501.1"
                     /translation="TETARMRLEAIRDFVDLGAGLKIAMRDLEIRGAGNMLGAEQSGH
                     IAAVGYDLYLRLLEDAKKQLQKSHDGKPMRPEAPPQECNVDLRVSGFIPEHYVPDEAL
                     RIDCYQRLVSAETIDQVKSIEHELQDRFGRPPPEVESLYELVKIRVLAQSMGINSIVE
                     DNLTLNFHFGPNVKLREDFLDRLVARFGERTTVRPNMIRIRKQRDNPVKLAREFIELA
                     CED"
BASE COUNT         2098 a         1647 c         1943 g         2016 t
ORIGIN      
        1 gtccgggaag agacgacatt cgccttctca ttaaaacgat caggggttca ttcaagaatg
       61 ccattttgag gtcatcaatc attgtcggtt tccctggtga aggcaggcag gattttgatg
      121 aactcgcctc ttttgttagg gaaacaatgt ttgacaggct cggggttttc aagtattcgc
      181 cagaagaaaa caccctctct tacaaaatga aacagatttc ttcgagggtt gcaagctatc
      241 gctggaacac cctgatggcc cttcaaagcg agatatcgct tgaaaacaac tcaaggctta
      301 agggacagat gttacctgtg atcattgatg gcatcggcaa ggacacggca atcgggagat
      361 ctatggctga tgcccctgag attgacggga cagtttcagt gaaaggcgca aacatcaaga
      421 ggggtgatgt tgtaaacgtc aaggtaaatt ctttctcgcc ttatgatctt ggaggtgtga
      481 aagttggcca ttagaacatt tattgccctc ggacttgatg cttcaatcct ggagtatatt
      541 gataaaataa tgcctgtgct caagagctct ggcgcaagcg caagctggac aagaaaagga
      601 aattttcatc ttacattgaa atttcttgga gacactgaag agagtattgc gcagaaaata
      661 tctgttgaac ttgaaggaat ggtttttcaa aaaatcccgt acaggctttc cagatttggt
      721 ggttttccaa acctcaggaa acccagggtt ctctgggttg gtcttgaaag cccaatgtgt
      781 attgaggttg cgcaaaaaat agatgctgtt tgcaacaaat ttggttttcc gcttgaagag
      841 aggatgtttg tgccacacct gactttgggc aggatcaggg agtcgtttga ttttgtgaaa
      901 actgttgtag aacttccacc accgccgcaa ggcgagcttt tttttaaaaa tgtggtattt
      961 tttaagtcaa ttctttcaaa ttcggggcca acatacgagc ctctctggtc aaaggagctg
     1021 ggatgagcga aaagatacct gaaaaaaccg aaagggacaa agctttagac tcggcaatag
     1081 cctccataga aaagcagttt ggaaaaggca ccgtaatgaa gctgggtgat ggccatggaa
     1141 gaatggccgt agaggccatt ccaaccggca ttttatcgat ggacatcgcc cttggcgtcg
     1201 gcgggttccc gagaggtaga ataattgaaa tatatggcgc ggaaggctct ggaaagacaa
     1261 ccatcgcact cacaactatt gcttcagtgc aaaagatggg tgggaaggca gtatttattg
     1321 atgcagagca tgcgcttgat ccttcttatg ccgagcgcct tggcgtcaac ctccaggaca
     1381 tcatcatatc ccagccagat tcaggtgagc aggcccttga aatagcagaa atacttacaa
     1441 gatcaggagc aattgacctg tttgtggtgg actctgtcgc ggcacttgtg ccgagggcag
     1501 agcttgaggg cgaaatgggt gactctgttg ttggccttca ggcaaggctc atgtcacaag
     1561 ccatgaggaa actggccggt gtaatgggca agagcaggac ttgtggcata tttatcaatc
     1621 agctcaggga aaagataggc gtgatgtttg ggaaccctga aacaaccact ggtgggaggg
     1681 cattgaagtt ctactcttct gtcagggtgg aagtaaggaa gggcgagagc caaaaagacg
     1741 gcgacagaat tgttggaacg atggtgaaat gcaaagtcgt gaaaaataag gttgccccac
     1801 catatctgtc tggggacttc gagattgttt ttggtcaggg tgtatcaagg gaatcccagt
     1861 tgttggacat gggagtcaat ctgaacatta ttgaaaaatc cggaacttgg ttctcgtttg
     1921 atggggaaag acttggccag ggacgtgaca acgccaagat gaccatatcc cagaagccag
     1981 aaatgatggc cgagattgaa aagaggatca ggagcgctta tggaatggcc ccgcagggac
     2041 agccccatag cgaagaaaaa taacagcaag gcttttacct actcactcag gttgcttgcg
     2101 gcaagagaca gggcagagct tgaaataagg gaaaagcttg caaagaaagg atttttagag
     2161 aaagaggccg aagaagctgt taagaaactc agaaaactcg gccttcttga tgaaaaaaaa
     2221 ttcataaaag agtatgtcag ggtcaaaaga gccaggcatt gggggccttt gaaagtcaag
     2281 cttgggcttg tggccttggg aatacaaaaa agtgatgccg acgcagaaat caggcaaaca
     2341 gactggagag aacctttgaa aaagcttgta aagtcaaaca agggtctcga aaaaagaaag
     2401 ctgtattcaa aactttccag gctcggtttc ccgtcagacc taataaaatc ggaaatgaag
     2461 tgcgaggaac aagatgattg aaaccattgt tggattgcta gggttggcac tcggtgccgc
     2521 agtggctttt ttctatgcca tgaatgcttc cgccaaatct gcggatggca agatacgaag
     2581 ggccgaagaa gagtgcaaga ggattgtttc gcaagccaag gacgaagcgg aaacgcagaa
     2641 gaaagaaatc cttgttaacg ccaaggacga ggcgatgaag cttgttgcag aaagagaaaa
     2701 agaattcaga cagcaacgcc aggagctggt agacattgaa agaagaatag cccagagaga
     2761 agaaaagatt gaacaaagat ctgaagctgt tggcaagcgt gaaaatacaa taacaaccag
     2821 agaaaaagag cttgacgaaa aacacgagca ggccgaccag attgttgaca agcagaggaa
     2881 agaacttgag agaattgctg gattaaacca ggaaactgca aaacagatca ttcttgatga
     2941 gctgaggcag aaaatgagtt acgagcttgc ccaaaaggca aaaaatatgg aagaagaagc
     3001 aaaagacaaa gcattgcaaa aggccagaga agttattctt ggctcagtgc agagaattgc
     3061 ttctgagtat acggcagagg tgaccgtttc tgctgttccg ctcccatccg aagagataaa
     3121 gggaaaaatc attggcaggg aagggcgcaa catcaggact tttgaaactg cgacgggcgt
     3181 tgaccttctc gttgatgata ccccagaggt tgtcaccata tcatgttttg atcctgtcag
     3241 gcgcgagatt ggaaagctgg cattggaaaa actgataaca gatggcagga tacatccagg
     3301 caggattgaa gaggttgtag aacaggccac aagggaattc gaggacaggc taaaactgga
     3361 aggcgaccag gcattgcttg aggtcggcct ccaacaggcc aagcctgagc tggtgaagct
     3421 tcttggaagg ctccgtttta ggacatctta tggccagaat gtccttgccc actcgattga
     3481 agtcgcaatg ttatcagctc acatggcaaa tgaactgggt cttgatgcca ggaaggccaa
     3541 aagagcaggc attttccacg acatcggcaa agccgttgac agggaaaggg aaggctcgca
     3601 tgacgagctt ggtgccgaaa tcctgaggaa gaatggcgag ccaatagaag tgattgaagc
     3661 tgccaggctc caccacagcg atattgcccc aacatccgtc tatgctgttc ttgtgcaggc
     3721 agctgatgcc atttctgcat caagaccagg tgcaaggcgc gaaagctttg aggcctacat
     3781 ccagcgcctt gaatcacttg agacgattgc aaagagcttc aggggagttg aaaaaaccta
     3841 tgcaatacag gccggcaggg aaataagggt aatagtgaaa ccaaaggaca ttgatgatgt
     3901 ccttgcatac cagatggccc gtgacattgc ggctagaatt gagaacgagc ttacatatcc
     3961 aggaaccata aaggttacgg tcataaggga agtcagatac actgagaacg caaaatgata
     4021 gtcctcttca ttggagacat agttggcaag cctggaagaa aggccattga agctgttgtt
     4081 ccaggccttg taaaacaata tggtgtcgac tttgttgtgg caaatggtga aaatgcagct
     4141 ggtgggtttg gccttacccc tgacatttca aacaaactca gagctgctgg catccatgga
     4201 ataacgcttg gcaaccatct ttatgaccag aaagaagctg acgaattgct tgaaaacgag
     4261 cgtcttctgg caaggcctgc caattttcct ccaggaaacc cgggcagtgg gcattttgtg
     4321 ctggaagggc ttgctggaac gcttgcagtg gtgagccttc agggcagggt cttcatgaca
     4381 cccatcgatt gcccgttcag ggtcgctgac aagatcgttg aagagctgag aagcacaaca
     4441 aaagctatcc tcatcgactt ccatgccgaa gcaacaagcg aaaaacaggc ccttggcatc
     4501 taccttgatg gaagagtctc tgcggttttg ggtacccaca cacatgtcca gacggccgat
     4561 gagagaatac tcaagggtgg gactggtttc atcaccgatg ttggaatgac tggcccccat
     4621 gaatcagtta tcggcatgga acccagcgcg ccgatctcca aaatgattag tgcaagacgg
     4681 gcaaggtttg aggttgcaga gggtgatgta aggttttgtg ccgttttgct cgacattgac
     4741 agagaaacag ggcaatgcaa aaagattgaa agaatgatga taaatgttcc ataaacgttt
     4801 tttgaataaa tagcaaaggc cctccattgg agggcctttt ttgttttagc ttgaataatt
     4861 atggctctgg gtttggtttt tttgccacaa actggattgt tggggccatg ccaaggattg
     4921 atctctcctg agcaagcctg aattccagct ccagaaagtc ttcaaagaag ccctcgtccc
     4981 agtctgttgc agatagtttt tcgccaggca gctttgtcag agcgtgttcc caaccataca
     5041 ggtcaacgac ctcaaatcct atggcctcaa gttcacaggc gatgtcgttt ggggtgaaaa
     5101 gttctttggt accccaccac tgggtttcgt ctattccgtt tgcaatgctt gacgcattgc
     5161 ttgttgattg ggggacgcag tcgactgctg ccgcaaatct tccaggtaca ctaccaaaaa
     5221 agaagccacc ctgcacaagg agcctcattg cttctcttga aattttttcc ggatcttcat
     5281 tgtcagagag ccagccagga caaattacga ccccgaagct ctctccatct attccttcgg
     5341 ttgtcgaggc tatacatgtt gggtcgtcat ctttttgcgg gcatattctt gccaccttgt
     5401 agccggcatt ttcaaggatg agcgccatct gggtctgttt tggtccatcg aggacaagaa
     5461 cattgcagat ttcctctgga agatagccga gaattccata gaggagggac ctgtttatga
     5521 tctctgatgc gaatgtgccc tcgttcattg agggggaggg attaaatttt gacatggttt
     5581 ttgcagaaaa aacttataga acggtcgtaa gttttttctt cctccacagt aaaataacag
     5641 gccgggagct cattttgtga tttgtggaac gatagacatt cacagcatct gtatttgcgc
     5701 tcgcacggtt catgggtaca attgcacttg gtgctatttg tttttttaca gctcacacta
     5761 atattttagg ttttttaagg tagtttttca agaacgggag gctcagagag aaattgttat
     5821 tgatggtcct ttttggcctt attccagatt tcatccatct ctcccaaagt catctggcca
     5881 attggttttc cggtctcgtt tgaatggttc tctatctttt caaatctttt tatgaatttg
     5941 tccaatgttc ttgaaagtgc cacttccggg cctacatcaa gaaatctggc cagatttaat
     6001 aaaacaaaaa aaacgtctcc aagttcgttt tcaacctcgc ccttgtcttt ggtctggatg
     6061 gcttctttga gctcttggat ttcttcatcc agtttttcca ggaccccttc ggttttgtcc
     6121 cagtcgaacc cgaggccctt tgcatgagcc attattcttt cggccctgcc gagtggcggg
     6181 agatttgatg ggatatccct tattgaccca ttgtttttat tctgcttttt gaggctgttc
     6241 cactggtcaa gaacagagtc aaccgttttt gtcttgctct ccccaaaaac atgtggatgt
     6301 ctgtaaacaa gtttttcctg cattgtggca attacatctt caacgctaaa tgattgctct
     6361 tccgagagca ttctcgagac catcagcacc tggagtagga tgtctccgag ttcttccttt
     6421 atgtgggcca tgtctttctg tttgactgct tcccagacct cataagtttc ttcaagaagg
     6481 ttttgcatca tgctgttagg ggtctgctcc ctgtcccagg ggcagtgcct tctgagcaga
     6541 tcaatgagga gctttgcttc atagaaagag atgtcggaag tgtttgtcaa aagtggcttc
     6601 acatactcac actcgttgac aaatccctga ttgtggagaa atgcaaccga gttgtcacct
     6661 cttattgcac gcacttcctc gattgcctgg tttgctgtca tattttcaaa tattacaaga
     6721 tagcatgcag ccagcgttgc agaacgttca taaccttcct ggcaatgaat cagcaggggc
     6781 aatccatgtt ttttcataaa tccggcccag aatatgaatt ccctcaactg ctcaagtgtt
     6841 ggtggtttgt cttccggaat ctggagcttg agattgaaaa gccccagatc ttcaaaatct
     6901 ttatcggtga atggtaactg ggaaagtgaa accacagctt ttatgccagt gtctctgagc
     6961 cttgaaagca tttgtatgtt ctctggagct gatgaaatca tcagctcctt ctctattatc
     7021 caggaaaagt tcatttttaa tcctcgcagg caagctcgat gaattctctt gccagcttta
     7081 cggggttgtc tctttgtttt ctgattctaa tcatgtttgg cctgacagtg gtcctctcgc
     7141 caaaccttgc cactagcctg tccaggaagt cttccctgag ttttacattt ggaccaaagt
     7201 ggaagtttag tgtcagattg tcttcaacga tggagtttat tcccattgat tgggcaagaa
     7261 cccttatttt tacaagctca taaagtgatt ccacctctgg cggtggtctt ccgaacctgt
     7321 cctggagctc gtgttcgatt gatttcacct gatcgattgt ttcagcgctc acgagcctct
     7381 ggtagcaatc aatccttagc gcttcgtcag gaacgtagtg ttctggtatg aaacctgaaa
     7441 ccctgaggtc aacattgcat tcctgcggtg gtgcctccgg tctcataggt tttccatcat
     7501 gtgatttctg gagctgtttt tttgcgtcct caagcaacct aaggtacagg tcatagccaa
     7561 ctgcggctat gtgcccggac tgttctgctc caagcatgtt gcctgcgccc cttatttcga
     7621 ggtccctcat tgctatcttg aggcccgctc cgagatcaac aaaatccctt attgcctcaa
     7681 gcctcattct ggctgtttct gtca
//
LOCUS       DAOSRI010000042         7634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9013_length_7634_cov_3.516031, whole genome shotgun sequence.
ACCESSION   DAOSRI010000042 DAOSRI010000000
VERSION     DAOSRI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_7634_cov_3.516031"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK717_02165"
     CDS             <1..323
                     /locus_tag="PK717_02165"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOR46502.1"
                     /translation="YIAWINLYGSDTEHYRIRTSSVVLGTKKDADFMIDDETVSPEHA
                     RISETGDGFVIVDLKSDSGTFVNDKKISGPVLLKNGDIIVLGNTKIEFTQSDFAYVCK
                     KKVI"
     gene            326..2023
                     /gene="hutU"
                     /locus_tag="PK717_02170"
     CDS             326..2023
                     /gene="hutU"
                     /locus_tag="PK717_02170"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002033512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOR46503.1"
                     /translation="MPQYNISYEKDAPAEQIKARRGKELVCKGWVQEAALRMLQNNLE
                     QTTKWRELIVYGGTGRAARGWQAYDAIVKALMRLENDETLLVQSGKPVGIFKTHEDAP
                     RVLISNSMIVPFWSTQEKFDELERKGLMMYGQMTAGSWIYIGTQGILQGTYNTLAAVA
                     EKHFGGTLKGTITVTGGCGQMGGAQPLAVTMNEGVCLIVDVDEGMIDQRINEGYLDCK
                     TNSLDEAVAMAKKAKEEGRAVSIGLVANCADVLPEFVKRNFVPEVLTDQTSAHDMLGG
                     YVPNGMPYSEALKLRASDPKKYLEFSYQTARKHVEAMTDLMRMGSITFDYGNNIRQQA
                     VKSGLAYEKAFSFKGFVPAYIRPLFCEGKGPFRWAALSGDPEDIYRTDELVLNMYKDD
                     KSLVRWIKMVREKLNFKNIPGLPSRICWLGYGERNRFGVAVNEMVKKGIISAPIVFGR
                     DHLDCGSVASPNRETEGMKDGSDAVADWAILNALTSTSCGASWVSVHGGGGVGIGFSV
                     HAGQVTVADGTDAAAKRLNRVLIADPGMGVIRHVDAGYEEAIEVAKKKGVRIPMWEI"
     gene            complement(2071..2757)
                     /gene="hypB"
                     /locus_tag="PK717_02175"
     CDS             complement(2071..2757)
                     /gene="hypB"
                     /locus_tag="PK717_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOR46504.1"
                     /translation="MDLELSRKVDFRHEEIASRIKEKLYKNGTLCLNFISSPGSGKTS
                     LLERTLDALKETYNLAVIEGDPETDRDARRLEKLHVPVILINTSGTGASCHLSPAEIE
                     QAIDDLLLETPPPDIIFIENVGNLVCPAAYDLGEHHKVLLFSVPEGCDKPAKYPVAFR
                     ESDLVLINKMDLAPYCDFDLESLKKDALSVNPSLEFITMSIKKGDGLSEWFSWIEKKL
                     GEVRKRTNSK"
     gene            complement(2747..3100)
                     /locus_tag="PK717_02180"
     CDS             complement(2747..3100)
                     /locus_tag="PK717_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOR46505.1"
                     /translation="MHELGIARNVLEIIIGEADQRGRRAKSVFLKIGQISGIVPESLE
                     FLMKCLSEGTPVEGLEVKIDIVKPVMVCKSCGEKFTVEELSFYCPKCGGGDCETIEGK
                     DLVIETITLEGDNGP"
     gene            3233..4726
                     /locus_tag="PK717_02185"
     CDS             3233..4726
                     /locus_tag="PK717_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR46506.1"
                     /translation="MDPSHDLTEGSIFKNLVKLSWPGALGRLFENLYDVVDMFWIGTL
                     GGVLATQAQAGILFFNVANSFMQMLNSIFGPGSITVISRFWGAKDYEKAAWASEQTIL
                     YKFLAGLAGTIISLFTLPYLVRFAGAQTDLVVGCACSTFDLALLYGWFIVAALPLIFV
                     YYTINTIFRCCSDAESSMFVIATSSLVNIVIDPLFIHGFGPIPKMGIVGAALATVLAY
                     VYAVTIGIYMLCNGLKFKISRFTFLALPYKKGDYYKYFIRIPYWKFHFEKGGIKIVFG
                     RLFKPDFQILINYLRIGFTPTVTQFVGSSSQIIFMNLISNFGQSVVTAFGIGGKVAGL
                     VNLPLLGLQQATSALVGQNLGAKKPERSEKTGWYSVLIGVCLGFVMVVVGYFFAPQIF
                     WVFNKDPAVIAVGKSVFALSMIGNMINAAQWMLWAVFEGSGYTMWPSIFGQVNNWLFN
                     ILLVYLAVKVFDQGYVWIFYIGIFSAIMSTITNTFLVRKGSWKRARV"
     gene            4913..7252
                     /locus_tag="PK717_02190"
     CDS             4913..7252
                     /locus_tag="PK717_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46507.1"
                     /translation="MIYSNFQPRKLTNYEHTQNAPLIHGGYMSWLDTRLHDREGKIMP
                     RIYMMDLVRGEEKATPNYAKKSGARAQYRNRLVWAENTDRLRVDNYDIKIFDFKKGTT
                     DYVCKEKNKQDNPQISENFVIWRDWRDVPKGNENSMSFAIYGLPSEGGEEFLVKQFDT
                     PGTGELFGNFVAMTVKNPRGDWDIQLFNCSNKKIIPVCNDFGDQITPKMVRDVVVWLD
                     NRNSQETPDSRQTDIYGYNIATKKTLTISTNLNREYGLVIGADRYVFWYEDTEPNFAG
                     VIRPIIKGYDVLTSKMITVTTEPGNYRNVCADGQYVIYEVITGSDRFGSDIMAFNLST
                     GQTYCVAKGIGDQRNPRIYGDFIVWEDQLSQDDEEVSIWGTCLSFPRESPEQVFYGYQ
                     EKKVWTMFRGDQKHNGTSAMDIKPIDKFPFILQWQVDLKEAVYSTPCFSKNGYAYIGC
                     DDGSMYKIAVSDGSLVWKFKTSGKIRSSPAIYRERLFFGDQNGKFYCMDTNTGQVIWE
                     YQTAGPIDGSPTVFQSDTDHYGCVVIGSGDRKLYMFNALSLAPMIKWTIDLGGWSYGA
                     PTVDYNQFVRYSDQEPVQKVLYVGVSNNRILCVQCSTGQILSEFKTESSIEATPVCDG
                     ATVMTGAKDGNFYGLHFFAWGQKPYVFFKQSTEHQLTSSAILDQTGCRVIYEGRSGWI
                     YSFKNSLQWSLKIDESMRSSPILINCAQLPVIFAGSDSGMFFMVDAKTGKPLWSEKLT
                     GSVVASPSVYDNGFVCVLVATTDHRLSCFGQRPKYPDDW"
     gene            7331..>7634
                     /gene="rplU"
                     /locus_tag="PK717_02195"
     CDS             7331..>7634
                     /gene="rplU"
                     /locus_tag="PK717_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOR46508.1"
                     /translation="MYAIIKTGGKQYKVAAGDTIKVEHLDAKVGEDYALDQVTLIENN
                     GNINVGKPFINNAKVVAQVMSHGKGRKLVAFFYRHKTNHRRRKGHRQEYTQLKIKEI"
BASE COUNT         2169 a         1512 c         1819 g         2134 t
ORIGIN      
        1 attacatagc gtggataaac ctctatgggt cagacactga gcattatagg atcagaacaa
       61 gctccgttgt gcttggaacg aaaaaagatg ccgacttcat gattgatgat gagactgtct
      121 cacctgaaca tgccagaata agtgaaactg gagatggttt tgttattgtc gacctgaaat
      181 cggattccgg gacttttgta aatgacaaaa aaatcagtgg cccggtgctg ctgaaaaacg
      241 gtgacatcat tgtccttggg aacacaaaaa ttgaattcac tcaaagcgat tttgcttatg
      301 tatgcaagaa aaaggtgatc tgataatgcc gcaatacaat atttcctatg aaaaagatgc
      361 gcccgccgaa cagataaagg caagaagggg aaaagagctt gtatgtaagg gctgggtcca
      421 ggaagccgca ttgagaatgc tccagaacaa cctggagcag accacaaaat ggagagagct
      481 cattgtctat ggcgggaccg gaagggcagc aagaggctgg caggcctacg acgcaatcgt
      541 aaaggccctc atgcgccttg agaatgatga aacattgctt gtccagtccg gaaagccggt
      601 tggcatattc aaaacacacg aggatgcacc aagagtcctc atctccaatt cgatgattgt
      661 tccattttgg agcacacagg aaaaatttga cgagcttgaa agaaaaggtc tgatgatgta
      721 tggccagatg actgcaggct catggattta cataggtacc cagggaatat tgcagggcac
      781 atacaatacc cttgccgctg ttgccgagaa acactttggt gggacactca agggtacaat
      841 aacggtgact ggtggatgcg ggcagatggg tggtgcccag ccacttgctg tgaccatgaa
      901 cgagggtgtc tgcctcattg ttgacgttga cgagggaatg atagaccagc gcatcaatga
      961 aggttatctt gactgtaaaa ccaacagtct tgatgaagct gttgcaatgg ccaaaaaggc
     1021 aaaggaagaa ggaagggctg tttccattgg ccttgttgca aactgtgccg atgtcctgcc
     1081 agagtttgtc aagagaaatt tcgtcccaga agtgcttaca gaccaaactt ctgcgcatga
     1141 catgcttgga ggatatgttc cgaatggcat gccttacagc gaagcattaa aactaagggc
     1201 ctctgatccg aagaagtatc ttgagttttc ctatcagact gccagaaagc atgttgaagc
     1261 aatgaccgat ctcatgagaa tgggatcaat aactttcgat tatggaaaca acataagaca
     1321 gcaggctgta aaatccggtc ttgcctatga gaaggctttt tcttttaagg gttttgtccc
     1381 agcttacatc aggccgttat tttgtgaagg caagggcccg ttcagatggg ccgcgctttc
     1441 aggtgaccct gaagacatct acaggacgga tgaactcgtt ctcaacatgt acaaagatga
     1501 caaatcactt gtcagatgga taaagatggt cagggaaaag ctcaatttta aaaacattcc
     1561 aggcctccct tcaagaatat gctggcttgg atatggcgag agaaacaggt ttggtgttgc
     1621 agtcaacgag atggtaaaaa aaggtatcat ctcggccccg atagtttttg gaagggacca
     1681 tctggactgt ggctcagtag cctctcctaa cagggaaact gagggaatga aagatggcag
     1741 cgatgcagtc gccgactggg cgatccttaa tgctttgaca agcacatcct gcggtgcctc
     1801 atgggtctcc gttcatggtg gcggaggggt tggtataggc ttctctgtcc atgctgggca
     1861 ggtgactgtt gctgatggaa ctgatgccgc ggcaaagaga ttgaacagag ttttgattgc
     1921 tgatcctggg atgggtgtca ttaggcatgt tgatgccgga tacgaggaag caatcgaggt
     1981 ggcaaagaaa aaaggtgtca ggatccccat gtgggaaatc tgataaaaaa gatacagact
     2041 atcaaaaagg cccctcaaag gggccttttt ttatttacta ttggtgcgtt ttcttacttc
     2101 accaagtttt ttctcaatcc atgaaaacca ctctgaaagg ccatcacctt tttttatcga
     2161 cattgtaata aattcgagtg aagggttaac agacagggca tctttcttca aggattcaag
     2221 gtcaaagtca cagtatgggg caaggtccat cttatttatc agcacgaggt ctgactccct
     2281 gaaagccacc gggtattttg ctggtttatc acaaccctca ggtactgaaa acaacaaaac
     2341 tttgtgatgt tcccccagat cgtaagcggc gggacagaca aggttaccga cgttctcgat
     2401 gaagattatg tctggtggtg gtgtttcaag gaggagatcg tctattgctt gctcaatttc
     2461 agccggagaa agatgacaag acgccccggt tccggatgtg ttgatgagta tcactggcac
     2521 atggagtttt tccagcctcc ttgcgtccct gtctgtctca ggatcaccct ctatgactgc
     2581 cagattgtat gtttccttga gcgcatccag tgttctttca agtagcgagg tttttcctga
     2641 acccggagaa cttataaaat tgaggcagag ggtgccattt ttatagagct tttcttttat
     2701 ccttgaagct atttcttcgt gcctgaagtc aacttttctt gatagttcaa ggtccattgt
     2761 cgccctccag tgtaattgtt tctattacaa gatccttgcc ctcaatggtt tcacaatcgc
     2821 cacccccgca cttggggcaa tagaatgaaa gctcctctac ggtgaatttc tcgccgcatg
     2881 acttgcaaac catcactggt ttgacgatat cgatttttac ctcgaggccc tctactggag
     2941 ttccttcaga aaggcatttc atcaggaatt cgagtgattc tggcacaatg ccggatatct
     3001 ggccaatttt caaaaagaca gattttgctc ttctgcctct ctggtcggct tcgcctatta
     3061 ttatctcgag gacgtttctt gctataccaa gttcatgcac aatttcatta tatagaaaca
     3121 ttcttctcta atccagacat gaaaaaatca caaactgaaa acactctttt aacattatct
     3181 ttttagggtt ccttgttagc attgtccaaa ctgcataaaa tctctctcta ttatggaccc
     3241 aagccacgac ctcactgagg gcagcatttt caaaaactta gtaaaactat catggccagg
     3301 tgctctcggg agactcttcg aaaaccttta tgatgtagta gacatgttct ggataggcac
     3361 gcttggtgga gtgctggcca cacaggccca ggcagggata ctctttttta atgtcgccaa
     3421 ttcatttatg caaatgttga attcgatttt tgggcctgga tctataacgg tcatttcgag
     3481 gttttgggga gccaaggatt atgaaaaggc tgcatgggct tctgaacaaa caatccttta
     3541 taaattttta gctggtctgg ctggtacgat cataagtttg tttactcttc cctatcttgt
     3601 gagatttgct ggtgcacaaa ctgatcttgt tgtcggttgt gcttgtagca cttttgactt
     3661 ggccttgtta tacggatggt ttattgtagc tgcgttgcca ctaatcttcg tttactacac
     3721 gataaataca atttttaggt gttgttctga tgctgaatca agcatgtttg ttattgccac
     3781 gtcttcgctt gtcaacattg taattgatcc cctttttatt catggttttg gtccgatacc
     3841 caaaatggga attgttggtg ccgcattggc gacagttctt gcgtatgtct atgctgtgac
     3901 aatcggaata tatatgcttt gcaatggctt aaaattcaaa atatcaagat ttacattcct
     3961 cgcgttgcca tacaagaaag gtgattacta caaatatttc atcagaatac catattggaa
     4021 gttccatttt gaaaaaggtg gaataaaaat tgtttttggg agattgttta aaccggactt
     4081 ccagatattg ataaattatc taaggattgg ttttacgcct actgttacgc aatttgttgg
     4141 ttcaagctcg cagataattt tcatgaatct tatttcaaat ttcggccaga gtgtagttac
     4201 cgcttttgga ataggtggaa aagttgcggg gctggtaaat cttccattgc ttggtctcca
     4261 gcaggcgacc tcggccttgg ttggtcagaa cctgggcgca aaaaagccgg aaaggtcgga
     4321 aaagactggc tggtattctg tactaatagg agtttgcctt ggttttgtaa tggttgttgt
     4381 cgggtatttt tttgccccac agattttttg ggtatttaat aaagacccag cagtaattgc
     4441 ggttggaaaa agtgtttttg cgctttctat gattgggaat atgataaatg ccgcacaatg
     4501 gatgctctgg gctgtatttg aagggtcagg atacacaatg tggccgtcaa tatttggcca
     4561 agtgaacaac tggctgttta atattctcct tgtctatctg gcagtaaaag ttttcgatca
     4621 gggttatgtg tggatattct atattggcat attttccgca attatgtcca caatcactaa
     4681 caccttcctg gttcgaaaag gcagctggaa aagagcgagg gtctaagaaa cattggagct
     4741 attgcattat tgaactccag tatctaaaat tagtaattaa atgccatcct gcaatcgcca
     4801 tttaagtaac aggccggcct tgtgctggct tattttcttt ttgatggcaa ttttaatttc
     4861 tcctcatgat ttctggacaa taccgaactc taatgctggt atcacgaaga ggatgatata
     4921 ttcaaatttc caacccagaa agctgacaaa ctacgagcat acgcagaatg ctccgctgat
     4981 tcatggtggt tatatgagct ggctcgatac cagactgcat gatcgcgagg gcaagataat
     5041 gcctcgtatc tacatgatgg atctggtccg tggtgaagaa aaagcaacac caaattacgc
     5101 aaaaaaatct ggggctaggg cacagtacag aaacaggctt gtctgggcgg agaatactga
     5161 cagactgagg gttgacaact acgacataaa gatttttgat ttcaaaaagg gcactactga
     5221 ctatgtgtgc aaggaaaaaa ataagcagga caatccccag atttctgaaa attttgtaat
     5281 ctggagagac tggagggatg ttcctaaagg caacgaaaat tcaatgtctt ttgccattta
     5341 tggtctacct tctgaaggtg gcgaggagtt cctcgtaaaa caatttgata cccctgggac
     5401 aggtgaactc tttggcaatt ttgttgcgat gaccgtaaaa aatccaagag gcgactggga
     5461 tattcagctt tttaactgtt cgaataaaaa aataatccca gtctgtaatg attttggcga
     5521 ccaaatcact cccaaaatgg taagagacgt tgttgtatgg ttagacaata ggaattcgca
     5581 ggaaacccca gattcgaggc agaccgatat ttacggttac aacatagcga cgaaaaagac
     5641 ccttacaatc tctacaaatc tgaacaggga gtatggcctt gtgattggtg ccgacaggta
     5701 tgttttctgg tatgaggaca ccgagcccaa ctttgctggt gtaatcagac cgattattaa
     5761 gggttatgat gtgttgacaa gtaagatgat aacggtaaca acggagccag gcaattacag
     5821 gaatgtctgt gcagacgggc agtatgttat ttatgaggtg attactggat ctgacaggtt
     5881 cggttctgac ataatggcct tcaatctgtc cactggccag acatattgtg ttgcaaaagg
     5941 tataggtgat caaagaaatc caaggatcta cggtgatttt attgtatggg aagatcaact
     6001 gtcccaggat gatgaggaag tatcgatatg gggaacttgc ctcagttttc cacgggaatc
     6061 gccagagcag gttttttatg ggtatcagga gaagaaagtc tggacgatgt tccggggaga
     6121 tcaaaaacac aatgggacgt cagccatgga cattaaaccc attgacaaat tcccatttat
     6181 cttgcagtgg caggttgatc tgaaagaagc ggtatactca acgccatgtt tctcaaaaaa
     6241 tggttatgcc tatattggtt gtgatgatgg cagcatgtac aagatagccg tgagtgatgg
     6301 ctctcttgta tggaaattca aaacatccgg taaaataaga tcttcgccgg caatctacag
     6361 agaaagacta ttttttggtg atcagaatgg caaattttac tgtatggata caaatacagg
     6421 ccaagtgata tgggaatacc aaacagcggg tccgattgat ggttcaccaa cagttttcca
     6481 gtcagacacc gaccattatg gttgtgttgt gataggttct ggagacagaa aactttatat
     6541 gttcaatgcc ctgtctctcg caccaatgat aaaatggaca attgatcttg gtgggtggtc
     6601 ttatggtgca ccaaccgtag attacaacca gtttgtaaga tacagcgacc aggaaccagt
     6661 gcaaaaagtg ctgtatgttg gcgtttccaa caacaggatt ttgtgtgtgc aatgctcgac
     6721 agggcaaata ctgtcagaat tcaaaactga atcatcaatt gaggccaccc cggtttgtga
     6781 tggagcaaca gttatgactg gagccaagga tggcaatttt tatggtttgc acttttttgc
     6841 ctgggggcag aaaccatacg tcttttttaa acaaagcacc gaacaccagc ttacttcatc
     6901 ggccattctt gaccagactg gttgcagagt catttacgaa ggaagatcag gctggatata
     6961 ttctttcaaa aacagtcttc agtggagctt gaaaattgat gaatcaatga ggtcgagccc
     7021 catacttata aattgcgccc agttaccagt gatctttgca ggttctgatt cggggatgtt
     7081 ttttatggtt gacgccaaaa caggcaaacc actctggtct gaaaaactta cagggtcagt
     7141 ggttgcatcg ccatctgtgt atgacaatgg ttttgtatgt gttttggtcg caacaacaga
     7201 tcacagattg agttgttttg gacaacgccc aaaatatccc gacgactggt aaattatcaa
     7261 accttttttc ttgacaagaa ttacctatca gatagcctta ttaagttgga actattgaaa
     7321 tggaggaccc atgtacgcaa ttattaaaac aggtggaaaa cagtataaag tcgctgcagg
     7381 cgacacaata aaggtcgagc atctggatgc caaagttggc gaggattacg ccctcgatca
     7441 ggtaacgctc atcgaaaaca atggcaacat aaatgttggc aagccgttca tcaataatgc
     7501 caaggttgtt gcccaagtga tgagccatgg taaaggaaga aagctcgtcg cgtttttcta
     7561 caggcacaag accaatcatc gcaggaggaa aggccatcgc caggaataca cgcagcttaa
     7621 aattaaagaa atag
//
LOCUS       DAOSRI010000043         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9024_length_7626_cov_3.364549, whole genome shotgun sequence.
ACCESSION   DAOSRI010000043 DAOSRI010000000
VERSION     DAOSRI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9024_length_7626_cov_3.364549"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK717_02200"
     CDS             <1..717
                     /locus_tag="PK717_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOR46509.1"
                     /translation="WVKTHIFETMEEGKRDILECAKYLASRGWAEAGAGNISIRTKHA
                     VVNNTNTIPINYPAEPDLAIYVTKSGSNFRLMAEDDVSSVVVNEDGQNITHGFKQGKP
                     TSELSTHLLLHSHFVGKNSLAIVHAHLTNLVAATHIFRSEQELNKAIFCHTEMPLLVP
                     GGIGLVPVIPPGSVDIAQATLEKIRQGKTCVAWACHGIVAIADNLLGATNIVEAVEKA
                     CEIALKIKSAGGKTIFVGQL"
     gene            complement(732..818)
                     /locus_tag="PK717_02205"
     tRNA            complement(732..818)
                     /locus_tag="PK717_02205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(782..784),aa:Leu,seq:cag)
     gene            1007..2275
                     /gene="eno"
                     /locus_tag="PK717_02210"
     CDS             1007..2275
                     /gene="eno"
                     /locus_tag="PK717_02210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOR46510.1"
                     /translation="MNRIEDVYARQILDSRGNPTVEVDCETDDGILARAAVPSGASTG
                     IREALELRDQDPKNYKGKSVEKAVLNVNETISAELYDHDVLDQRGVDSLMNELDGTDN
                     KSDMGANAILAVSMAVARAGAATCDIPLYRYLGGAMANRLPVPFMNVINGGVHADNNL
                     DFQEFMIAPSGSASFSEALRMGTEIYHTLKAILKKNGLSTAVGDEGGFAPNLKNHEEA
                     LKVLVQAITEAGYVSGKDCSIAMDAASSSIYKDGKYGFEGKKLSNAELNSWYLEMVSK
                     YPIISIEDGMAEEDGEGWKIHTESLGGKIQLVGDDVFVTNPAIFAEGIKNGIANAILI
                     KLNQVGSVKETWDTIELARRNGYGYMISHRSGETADSFIAHLAVASSSGQIKTGAPCR
                     SERVEKYNELLRIEEELGQSAIYGLPEKER"
     gene            2275..>4463
                     /locus_tag="PK717_02215"
     CDS             2275..>4463
                     /locus_tag="PK717_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46511.1"
                     /translation="MKKFFIALLALTMVMPTVVFPAFSETTDWPEPFYNQTHANNSNG
                     PAVPIPLQKSWAFYPEGDSLGMPIAIGDSIIVSDTMGYIVSLDKKTGQPKWSYDIGFE
                     AQTLVAGSGDKLAFATASVMMVSGRRRGGRGGRGGPLSLSGINQFLQFPNSTREDETQ
                     KMYFGVIDIKTGKELWKKEPESGLSIGQGCISGNKIIIPFSVMKTNGSIESKLIAYSI
                     SDGSQIWAIDYEKSMASSMPTIAEGKIFIGAMTIKIPQEDDPVNQTPGMKDPEILCLD
                     ESNGKVVWKKGGFEENLMGIISYADGTLYTCLTKIDLTGGGGGRFTMPESNVSAFSAS
                     DGTRKWIRKIEGDFMTTGIMAVTKYGLVVQCTMSSTMCLDLKEGTVKWNQEKTPGGMC
                     FGIQYVCNGEQVISTRQSKISVINLSDGKIVFQEDTGMKSGFGMMGGDMVLSYPIVSG
                     DKVFVAGDKLICYGEKIIGLSSDPDRIKLNIEAGQKKTLKLKLVYNAQAEIKGTITTS
                     QPWIKLSANDYSTASQTYEVTLSTEGLEPGVYKGSIDVDASVGKLSISVEMTVVPKPP
                     IKLEVNLEDPTYTNKNPFKVTGQTVPGAKLTISGRPANVASDGSFAEEIPLEQGSNKL
                     TFEARDSKGNVANATRTVIFDDKKPQLNISLKDGDTIKKFPLTVSGQTEKNARVKVNG
                     VPVEVKDNGEFEAVIEELEQGEQTITIIAEDLAGNVTKIERKVLVAT"
     assembly_gap    4464..4473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4474..5406
                     /locus_tag="PK717_02220"
     CDS             <4474..5406
                     /locus_tag="PK717_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46512.1"
                     /translation="DQKSPILTNNPNITITGQTEAGASIAAIVFGGKPTDPVTAGQDG
                     KFSITVALEEEGRFRIILQVMAENGQSTNANFDVLFDKTPPLIESTIPAVSKEKTVEI
                     KGKLSEYCMLTINDNPVEVGKTDFTFSTKVELKPGLNSIIIKATDLVGNVSKLEGFTK
                     FEDATQVQTVVIKLWVDKQDMTINGKTTKLAVAPTTSSPPLPKQLAGSTYMPIREVAE
                     ALFASVGWDAKEQKVTLTQKTPDGKTKIIELWISKKMAKIDGKEINIDSQGKLYPTIV
                     QGKTMLPLRFVGEALGAKVEWDGKTKMITLTFPK"
     gene            complement(5496..6470)
                     /locus_tag="PK717_02225"
     CDS             complement(5496..6470)
                     /locus_tag="PK717_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR46513.1"
                     /translation="MNKRIIALLITLAVVPFGFVACGEKKEKAFMSGVVYEESTKTPL
                     SGATVYLNDARLDTGADAQFTFSELEPGATYKLKVTKEYFETFEEDIIAVYPTTTKEV
                     NLKLKKVAEGFVSPIKEVSVAKLKSYDFDIAFGSDSKDIALTMTGSFVAPSSYTFEMK
                     SKERSSTPEINNGKPEEPQERVKKAVQIGNNQWIDDGSGYRPSNEWFPGFNDIFSIIN
                     LDVSRLTKSFKAPQAFVDNGMVQIGDKQCRQLRGVSIIEEQMDSKSNKTQKLFVEFNV
                     AIIESGELAGVPVQTELITYQSAREVKTWSKVTLSNFNIPSEIKPPIK"
     gene            6682..>7626
                     /locus_tag="PK717_02230"
     CDS             6682..>7626
                     /locus_tag="PK717_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46514.1"
                     /translation="MRNKFIKLMALLLVLGMFFTTSGTAYALLGPVHIELNVMGERPS
                     GVRAGQVTAFKIHIRFNVNIKVHDWIKIWFPIDENSCDLKDICDGIPQVTGAKESPRF
                     VPNEAYFAKYQNKDESDVGKLYEVLDDRKGLTRFDKCECSAAAKDYGNCAQEGKCRII
                     SDPTGLGCWVTGSVLPALPRDEAERYKRLAQIIVTTSIGYTPCTACQGLPYLINTAKE
                     RSYQVNSPLEVEAWRKGYNPIDFNTSKGTGIIAPMTPGRYRLTIATAPEPTPVESEYF
                     VLPCSEISKPKVTLKPPDTDTVTEMKITFNTGEGGALDA"
BASE COUNT         2385 a         1663 c         1729 g         1839 t
ORIGIN      
        1 tgggtaaaaa cccatatatt tgaaaccatg gaagaaggaa aaagggacat cctggaatgt
       61 gccaaatatc tggcttcaag gggctgggca gaagccggtg caggaaatat aagcattagg
      121 acaaaacacg ctgtagttaa caatacaaac acaataccga tcaattaccc ggcagagcct
      181 gatctggcaa tctacgtcac aaaatcaggt tcaaacttca ggctgatggc tgaagatgac
      241 gtatcatcag ttgtggtgaa cgaagatgga caaaatataa cccacggctt caaacaggga
      301 aaaccgacca gcgagctttc aacacacctt ctcctccaca gtcatttcgt cggaaaaaac
      361 agcctggcaa tagtgcatgc ccacctgacg aatcttgttg ccgcaacaca cattttcagg
      421 tccgagcagg aactcaacaa agccattttt tgccataccg aaatgccgct ccttgtccct
      481 ggtggaatag gtcttgtgcc tgttatcccc cctggctccg tcgatatagc acaagcaaca
      541 cttgaaaaaa tcagacaggg aaaaacatgc gttgcatggg cctgccacgg aatagtggcc
      601 atagctgaca atcttcttgg agcaacaaat attgtcgaag cagtagaaaa agcgtgtgaa
      661 attgcgttga aaataaaatc tgctggcgga aaaaccattt ttgtgggaca attatgacgg
      721 gagagaaaag atggtgccga agaagggact tgaaccccca ccccgttgcc aggacatgga
      781 cctgaaccat gcgcgtctgc caattccgcc acttcggcaa cctttatgag gttatacaaa
      841 accgaaccca gcaagtcaag gagaaccttg gttttttatc atacaatggc tctgattcat
      901 gccagtagca aaaactgact ttttgagtgg tgttttgcgt actcaaagtt gagttttttg
      961 gaaactcctg caatattgtt tgtgtaaaaa caaaggagag taaaaaatga acaggattga
     1021 agatgtatat gcaaggcaaa tactcgattc cagaggcaat ccgacagttg aagtagactg
     1081 cgaaacagat gacggcattc ttgccagagc ggccgtacca tcaggcgctt caaccggaat
     1141 cagggaagca ctcgaacttc gggaccagga cccaaaaaac tacaaaggca aatcagtcga
     1201 gaaagctgtc ttgaatgtca atgaaacaat ctcggcagaa ctttatgacc atgatgtcct
     1261 cgaccagagg ggagtagatt ccctcatgaa cgagctggat gggacagaca acaaaagtga
     1321 catgggcgca aacgccatat tggcagtttc aatggcagtg gcaagggccg gagccgcaac
     1381 atgtgatatc cccctttacc gctaccttgg cggcgcgatg gccaacaggc tccctgttcc
     1441 attcatgaac gtaataaatg gcggcgtaca cgcagacaac aatctagatt tccaggagtt
     1501 catgattgcc ccctcaggtt ctgcaagctt ttcagaggca ctcagaatgg gtactgaaat
     1561 ctatcacacc ctgaaagcga tcctcaagaa gaatggcctt tcaacagctg ttggtgacga
     1621 gggtggtttt gcgccaaatc tcaaaaatca tgaggaagca ctaaaagtgc tggtccaagc
     1681 catcacggaa gcaggctatg tttccggaaa agactgctcg atagcaatgg atgctgcttc
     1741 atcatcaatc tataaagatg gcaaatatgg atttgagggc aagaagctga gtaatgccga
     1801 actgaattca tggtatctgg aaatggtttc gaaatacccg attatttcca ttgaagacgg
     1861 aatggcagaa gaggacgggg agggctggaa aatacatact gagtcacttg gaggaaaaat
     1921 ccagctggtc ggtgacgatg tgtttgtgac aaatccagcc atctttgctg agggtataaa
     1981 aaatgggatt gctaacgcca tcctgataaa actgaaccag gttggaagtg tcaaagagac
     2041 ttgggacacg attgaactgg ccagaaggaa tggttatggt tacatgatct cgcacagatc
     2101 aggtgaaaca gccgattcct tcatcgccca tctggctgtg gcatcatctt caggccagat
     2161 aaaaacaggt gccccatgca gatctgaaag ggtcgagaaa tacaacgaac tcctgaggat
     2221 cgaagaagaa cttgggcaat cagccatcta tggcctgccc gaaaaggaga ggtaatgaaa
     2281 aaatttttta ttgcgctttt ggccttaaca atggtaatgc ctactgtagt attcccggca
     2341 ttctccgaaa caacggattg gccagaacct ttttacaacc aaacccacgc aaataattcc
     2401 aatgggcctg ccgtaccaat cccgttacag aaatcctggg cattctatcc ggaaggtgat
     2461 tctcttggga tgccaatagc tatcggtgat tcgataatcg tttcggatac tatgggctat
     2521 atagtttctc tggacaagaa gaccggccag cccaaatgga gttatgatat tggttttgag
     2581 gcccaaaccc tggttgctgg atcaggcgac aaacttgctt tcgccactgc ttcagtgatg
     2641 atggtcagtg gcaggagaag gggtggtcgt ggcggcagag gtgggccatt atccttatct
     2701 ggaatcaacc agtttctcca gtttccaaat tcaaccagag aagatgaaac acagaaaatg
     2761 tattttggcg ttattgacat caaaactggc aaagaattgt ggaaaaaaga gccggaatcc
     2821 ggactctcaa ttggccaggg ttgcatcagt gggaacaaga taataatacc attttccgtt
     2881 atgaagacca atggctcgat agagtcaaaa ctcattgctt actcgataag tgacggctcc
     2941 caaatctggg caatagatta tgagaaatcc atggcatcaa gcatgcccac cattgcagag
     3001 ggaaaaattt ttatcggtgc aatgacaata aaaatcccgc aagaagatga cccagttaat
     3061 caaacaccag gaatgaagga cccggaaata ctttgcctcg atgaatcaaa tggcaaagta
     3121 gtatggaaaa aaggtggatt tgaagagaac ctgatgggga tcataagcta tgcagacggg
     3181 accctctata catgcctcac aaagatagac ttgaccggcg gtggcggtgg caggttcacc
     3241 atgccagaat caaatgtttc tgcattcagt gcctcggatg gcacccggaa gtggattcgc
     3301 aagatagaag gcgacttcat gacaactggc atcatggcag taacaaaata tggccttgtt
     3361 gtgcagtgca cgatgagctc aacgatgtgc cttgacctca aagaggggac tgtcaaatgg
     3421 aaccaggaaa aaaccccggg tggaatgtgt tttgggatcc agtatgtatg caatggtgaa
     3481 caggtaataa gcacaaggca gagtaaaatc tcggtcatta atcttagtga tggtaaaatt
     3541 gtcttccagg aagacaccgg gatgaaatct gggttcggaa tgatgggcgg cgatatggtc
     3601 ctttcttacc caatcgtgag tggcgataag gtttttgttg ctggcgacaa attgatatgc
     3661 tacggagaaa aaatcatagg tctctcgtct gatccagata gaataaaact aaatattgaa
     3721 gcgggacaaa aaaagacgct taaattgaag cttgtttata acgcgcaagc tgagattaaa
     3781 ggcaccatta cgacctcaca accatggata aagctcagtg cgaatgacta ttccacagca
     3841 tcacagacct acgaagtgac actctcaaca gaagggctgg agcctggggt gtataaaggc
     3901 tcaatagatg ttgatgcctc ggttggcaaa ctgtcaatat cagtcgaaat gactgttgtt
     3961 ccaaaaccgc caatcaaact cgaggtaaac ctcgaggatc ctacttacac aaacaaaaat
     4021 ccatttaagg taacaggcca gacagtccca ggtgcaaagc tgacaataag tggaaggccg
     4081 gcaaatgttg catctgatgg ttcttttgca gaagaaatcc cactcgaaca gggttcaaac
     4141 aagctcactt tcgaagccag ggattcaaag ggaaatgttg caaatgcaac tagaactgtc
     4201 atttttgatg acaaaaaacc ccagctcaac atatcactca aagatggcga cactataaaa
     4261 aaattcccat tgacagtttc cggtcagact gagaaaaatg caagagtcaa agtgaatgga
     4321 gtccctgttg aagtaaagga caatggtgaa tttgaagctg tcatcgagga attggaacag
     4381 ggtgaacaga caataaccat aatagcagag gatctcgctg gaaacgtaac caagattgaa
     4441 cgtaaagtat tggttgccac aatnnnnnnn nnngatcaaa aatctcctat tcttacaaac
     4501 aatccaaaca taacaattac agggcaaacc gaggctggtg cttcaattgc agcaatagtt
     4561 tttggtggga agcccaccga tccagtaaca gcaggtcagg atggaaaatt ctcgataaca
     4621 gtagcgcttg aagaagaagg tcggttcaga atcatacttc aggtcatggc tgaaaatggt
     4681 caatcaacaa atgcgaattt tgatgtgctt tttgataaga caccacctct catagaatcc
     4741 acaatccctg ctgtttcaaa ggaaaaaact gtcgagataa aagggaaact ttccgagtac
     4801 tgcatgctta caatcaacga taacccagtt gaagtaggta aaaccgactt cacattcagc
     4861 acaaaggtgg aactgaaacc aggtttgaac tccatcatca tcaaagcaac tgacttggtc
     4921 ggcaacgttt caaagcttga agggttcacc aaatttgaag atgcaaccca ggtacagaca
     4981 gttgtaatca aactatgggt tgataagcag gatatgacaa taaatggcaa aacaaccaag
     5041 ttggcagttg ctccgacgac atcatctccg ccacttccaa aacaacttgc aggatcaacc
     5101 tacatgccaa taagggaagt cgcagaagca ctcttcgcct cagttggctg ggatgccaaa
     5161 gagcaaaaag tgaccctcac tcagaaaaca cctgacggga agacaaaaat tatcgagctc
     5221 tggattagca aaaagatggc caagatagat ggcaaggaga taaacatcga ctcccaagga
     5281 aagctttacc caacaatagt tcagggtaaa acaatgttac cattgaggtt tgttggagaa
     5341 gctcttggtg caaaagtcga gtgggacggc aaaaccaaga tgatcacatt gacattcccg
     5401 aagtagtaat caagataatt tcccactata aataaaaaag gcccctctta aggggccttt
     5461 tttatttatc tggtaaggat tgagattttt tattactact tgatcggtgg ttttatctct
     5521 gagggtatat tgaagtttga aagagtaact ttcgaccagg tttttacctc tctggcactt
     5581 tgataggtaa taagctctgt ttgcaccgga acaccagcaa gttcaccact ctctatgatg
     5641 gcaacgttga attcaacaaa aagcttctgg gttttattgc ttttgctgtc catctgctct
     5701 tctattatcg atactcccct gagctgtctg cattgcttgt ctccaatctg gaccattcca
     5761 ttgtcaacaa aggcttgtgg ggccttaaaa gattttgtta accgactcac atccaagttg
     5821 ataatagaga atatgtcatt gaacccaggg aaccactcat tgctgggcct atagccggac
     5881 ccatcatcaa tccactggtt atttccaatc tgaactgcct tttttactct ttcttgtggt
     5941 tcttcgggtt ttccattgtt tatctcaggt gtactgctac gctctttgga tttcatttcg
     6001 aaagtatagc tgcttggagc gacaaaagat cccgtcatag taagggcaat atccttacta
     6061 tcactgccaa aagctatatc aaaatcatac gattttagtt ttgcaacact aacttccttt
     6121 attggcgaaa caaacccctc agcaaccttt ttgagcttga ggtttacttc tttggtagtg
     6181 gttggataaa cagcaataat atcttcctcg aaggtttcaa aatattcctt ggtcaccttc
     6241 agtttgtatg tcgcaccagg ttccagttcg gaaaaggtaa actgtgcatc ggcaccagta
     6301 tccaaccttg catcattgag atatacagtt gctcctgaaa gtggcgtttt tgttgactcc
     6361 tcatatacaa caccactcat aaaggccttt tccttttttt caccacaggc cacaaagcca
     6421 aaaggaacaa cggccagcgt aataagcaaa gcaataattc tcttgttcat acaacctcct
     6481 agcggttgaa gtttatttga tggcaaacac gtttcaatga acaaacttaa tccatggtta
     6541 actaatcgtt actccaaata caactaaagg ggtgaaggaa ttccgctatc ttctattaat
     6601 aatcacttta acctttgttg aacagatatc ttaaaaggct agaatgattc ggtagtcttg
     6661 cggaatcgaa aggagcttat catgaggaac aaatttatca agcttatggc cctgttgctt
     6721 gtgcttggga tgtttttcac aacaagcggg acagcttacg cacttcttgg cccggtccat
     6781 atcgaactga acgtaatggg cgaacgtcca tccggagtaa gagcaggcca ggtgaccgca
     6841 ttcaaaatcc acattagatt caatgtaaac atcaaggtcc acgactggat caagatatgg
     6901 ttcccgattg acgaaaactc atgcgacctg aaggacatct gcgacgggat cccccaggta
     6961 accggggcta aggaaagccc aagatttgtt ccaaacgaag catattttgc aaagtatcaa
     7021 aacaaggacg agtcagacgt tgggaagctc tacgaggtcc ttgatgatcg caagggactt
     7081 acaaggttcg acaagtgtga gtgctctgct gctgcaaaag attatggcaa ttgcgcccag
     7141 gaaggcaaat gcaggatcat ttccgatcca acaggccttg gttgctgggt gacaggttct
     7201 gttctaccag ctctcccaag agatgaagcc gaaagataca aaaggcttgc ccagataatc
     7261 gtcacaacgt caattggata cactccatgc acagcctgcc agggtcttcc gtatctcatc
     7321 aacactgcaa aagaaagatc atatcaggtc aattcaccac tcgaagttga agcttggcgc
     7381 aagggatata acccaatcga tttcaacacc tcaaagggca ctggaatcat tgcaccaatg
     7441 acgcctggaa gatacagatt gacaatcgca accgccccag aaccaacacc tgttgaatcg
     7501 gaatattttg ttcttccatg ttctgaaatt tcaaaaccaa aagtaacgct taagccgcca
     7561 gacaccgaca ctgtcactga aatgaagata acattcaata caggtgaagg tggtgcactc
     7621 gatgcc
//
LOCUS       DAOSRI010000044         7588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9075_length_7588_cov_4.762644, whole genome shotgun sequence.
ACCESSION   DAOSRI010000044 DAOSRI010000000
VERSION     DAOSRI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9075_length_7588_cov_4.762644"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2509
                     /locus_tag="PK717_02235"
     CDS             <1..2509
                     /locus_tag="PK717_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46515.1"
                     /translation="SVDCVLMKMTIKYNGLDILVMLQGATIKKDGATINCVDLKIGDT
                     VIVWGSIVPNEAKVIAVKVEVVTSGGGGDCNGPFTYTGKIEMVNCLGQPQTIILVVGN
                     TKKIIFITSSTKITIDGKTATCQQLKAGMTVTVTGNCVPDKGLYATEITSGQSESELG
                     TVSGILGGTSCYYKKISVMIDGEYDYTVFMIDDQTEIIVDGVKTTCAALRSGMRIVVT
                     YKAVGNPPTLVAVKIVATTPTNPPPPTPPPGGGSGGGSGGGGTPPTPPGGGGSGGGTT
                     PTPGDNEGQGFESVLTVTKIDCAGKTIEGTDEYNAGTKMTVKLSENSTINYKDQQITC
                     DKLKVGYLIKVNGVRGKGTNVNATTCEILPVSPLPNKINGRIVEVDKVKGWVKLEPVK
                     SGISTMSVSGGVKSDGDYVLVYIDPTTTFEYRGRKLTIQDLRPGVMMEISGLVDPINS
                     VMTTNTITLSARQAIDVTQKGRVADAFCDKSMFWLITDDPESKYVLIKLSTDTAVTLE
                     GSKATCADLVKGTEVTVTGKLDPFDLYVSAQKVEALKPQIGTATIEGEMMALDSTKQQ
                     LWIKISDEQGTRFVMVKYNTETKFLAFGKAGTVADLVYASVLTVTGSYDPSNQFIFNA
                     GTVESLGKSEENLSSTGTVVFADCVGKIIFIKTQKGVERFELVSSTKITSGDKPASCA
                     DIKEGQQVTITYDRKGTKVFTTKLTLPPPKTIIKLTVGLGVISVNGQNVLIDAPPYVK
                     GGTTMVPIRVVTAYFGASLTWGQADKRLTLQRGKDIIIVWIGRQEALVNGKSTPLAQA
                     PEYGAADRMMVPLRFFSEVFGAQVEWDQASKTITITL"
     gene            complement(2588..3298)
                     /locus_tag="PK717_02240"
     CDS             complement(2588..3298)
                     /locus_tag="PK717_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR46516.1"
                     /translation="MKAVVVYGSPRGKNSASYRLGSNFARGLEDVGHQVQEIMLNDYE
                     IGHCTGCKSCWTATPGKCAQNDGMAKIVEDHRNADLVVLSVPLYFFTVPGKVKDYLDR
                     QLPLYLETFHKSTSSTPKDSKTWLEKIKFVLISPCGLPEKSNFDGLKATMKQIFGSSY
                     AQDFLVPFADGMAKDNDETTFPDVYALMRDAGREFGKTLAISQETRDHFEQMTTMDKE
                     KMGQIIAALKARKQQKQS"
     gene            3485..4996
                     /locus_tag="PK717_02245"
     CDS             3485..4996
                     /locus_tag="PK717_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46517.1"
                     /translation="MKKCFSLFLLMSLLIFPLATGTSKTSDFGVATRSRGKLDFKSRS
                     PYVLFTCQREGFFEVRSLQTGKSVFCHGWVDYSHGPFLEDGKITFYDPDKGAKSNMRM
                     DFDGNVEYFDPTINQTNPPTRHKIVQNADKSYTLRVLSVDGDKEIWTMTTKSGEKIEY
                     KDGWFLYSGSGFMKVLNPITGEIALALEGKTVQFAGQNDDFGVFYVFDGPKDFVCQIL
                     DWKTLKLLVKTKIPSAVTPIFGNGEVIFVCRIDRRLPDAIKIESNWGWTRYTREKGIE
                     NFSFSVEGVFEPATNEVIDAKGYLLSFATIDPQKLVVVDARIGKAVFERPFYPEIANF
                     FGDVLYYQSWFDCGAVDTATWRTLWRFGFGGEAGEYDGYKYYVQNMVFGEKDEGPLLR
                     FEVIDLKTGLLEPYEYLVPYNWSEIVFTKHGIVTLPTEQSFSRGTRPGFALFAPGLAE
                     PIYSLHKNLGGWVKHYEITDNGEFLLLEVVTPSTGETRRYKLAIADGSLEFLK"
     gene            complement(5039..6397)
                     /locus_tag="PK717_02250"
     CDS             complement(5039..6397)
                     /locus_tag="PK717_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46518.1"
                     /translation="MSEAGQTGAGINKNLIYWVVIILVTSSLVLAAIILDPGRAKLKL
                     VASNTDREIVGYLYEDPKDTSKGYLPVVFDKSDCGLYILTENLKETLTNIESPGKLLS
                     INIKKDPIPGSHDTSTTNIAAIFESDTSNALVLYKVENVHTVFSDYYKHFENAFYVET
                     YADTIIGRYLPFDDNKTIILCTNKRGEVFHYSNTGELASYPFEPKTIFAENYKNGSSG
                     LIGYDGTNLIFYTVFKEKITPYKKIPLGTKGDIEKSFICGNTKNPDRGIISIYDGQKL
                     FLFDQRESRFIFSIKHDGVRPGLANMLFPFDGGLFFVNGCVSYFGGKQQVPQKAAYFA
                     YDGISQTKNAKLLTCTSFNENGGENAIFIVSYESLGKHKVLAYKATIFDYFTSKIHYL
                     SRTNFEDTPEEVETALFDAKGENCMLYSKNSVWSFGVAEAFRNRAQVTLHHRVYEYLE
                     EK"
     gene            complement(6446..>7588)
                     /locus_tag="PK717_02255"
     CDS             complement(6446..>7588)
                     /locus_tag="PK717_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46519.1"
                     /translation="QTKHKESKNYNNSDGDLIFQIAFDSNRVYLCRPEGKKNIDDGYP
                     DEGISLILSCLDVTSGKLIWEQRFFDPDETNQALYGTSRYMKAGNIFIKNNIVCAQIE
                     CHTYYYHWEGLCCFDASNGKKLYSLPLNYDENPFSDGKLRCRNMFIDIETGGCIWEDK
                     SYMDCYARSGDYAYASNSSSLDFSFFESSNLEIFNTLTNTKTGSFDFCFDGSSDNKKS
                     YINRRSFVVPIKDYLFVVNKPNYLYCFSEGAAKKLEFQIGSSKFKADGAEQDMGVKLQ
                     TIGGITYLPAKFVAEPMGGDTLWNDKWKEVTVKLNGNTIRMRIGHEKATVNIKEIQID
                     PKNPKVVPIISGGRTLVPMRFLAENLGCKLDYDQKTKKITITSGVK"
BASE COUNT         1967 a         1692 c         1816 g         2113 t
ORIGIN      
        1 atcggttgat tgcgtactaa tgaagatgac catcaaatac aacggccttg atattttggt
       61 catgctccag ggcgcaacaa taaaaaaaga tggtgcaacc ataaactgtg tcgacctcaa
      121 gattggcgat actgttattg tgtggggcag cattgttcca aacgaggcaa aggtcatcgc
      181 agtaaaggtt gaggttgtga cctcgggcgg tggcggtgat tgcaatgggc cgtttaccta
      241 taccggcaaa attgaaatgg tcaattgcct tggacaaccg caaacaataa tcctggttgt
      301 tggtaacaca aaaaagatca tttttataac ttcttcaaca aaaataacaa tcgatggaaa
      361 aaccgcaact tgccagcagc tgaaagccgg catgactgtt acggtcacag gcaattgcgt
      421 tccagacaag ggcctatacg caaccgagat aacctctggc cagagcgagt cagagctcgg
      481 gactgtcagt ggaatacttg gcgggaccag ctgctattac aagaagattt cagtcatgat
      541 tgatggcgag tatgactaca ccgtgttcat gattgatgat cagaccgaaa tcattgttga
      601 tggcgtgaaa accacttgcg cagcactcag gtccgggatg agaattgttg tcacctacaa
      661 ggcagttggc aacccgccaa cccttgttgc agtcaaaatt gtggcaacca cgccaacaaa
      721 tcctcctcca ccaaccccac caccgggtgg cggttctggc ggtggaagtg gcggcggggg
      781 tactccgcca acaccgcctg gtggcggtgg ctccggtgga ggcacaactc caaccccggg
      841 tgacaacgaa ggtcaaggtt ttgagtctgt tcttacagtc acaaagattg attgcgcagg
      901 caagacaatt gaaggtactg acgaatacaa tgccggaacc aaaatgactg tcaagttgag
      961 cgaaaatagc acaataaact ataaggacca gcagataacc tgtgataagc tcaaggttgg
     1021 ctatctgatc aaggtcaatg gagtcagggg caagggtacc aatgtaaatg ccactacttg
     1081 tgaaattctt ccggtttcac ctctcccaaa caagatcaac ggtcgcattg tcgaagtcga
     1141 taaagtaaag ggctgggtga agctggagcc ggtcaagtct ggcatcagca ccatgtcggt
     1201 gagcggcggt gtcaagtcag atggtgacta cgttctcgtc tacattgacc caaccacgac
     1261 ttttgagtac aggggcagga aactcaccat ccaggacctc aggcctggcg taatgatgga
     1321 gatttcgggg cttgttgatc cgatcaattc ggtgatgaca accaatacga taacgctctc
     1381 ggccaggcag gcaattgatg ttacccagaa gggccgcgtt gccgatgcct tctgcgacaa
     1441 gagcatgttc tggctcataa ccgatgatcc ggaatcaaag tatgttctca ttaagctttc
     1501 aactgatact gcagtgaccc ttgaaggcag taaagccaca tgtgctgatc tggtgaaagg
     1561 cacagaagtg acagtcactg gaaaattgga cccgtttgat ctttatgttt ccgcgcaaaa
     1621 ggtcgaggcc cttaagcccc agattggtac agcgaccatc gagggtgaaa tgatggctct
     1681 ggattcaaca aaacagcagc tctggatcaa gatttcagac gagcagggca cgagatttgt
     1741 tatggtcaaa tacaacaccg aaaccaagtt cctggctttt gggaaggccg gaacggtggc
     1801 cgatcttgtc tatgcgagcg tactcacggt gacaggctcc tatgacccga gcaaccagtt
     1861 catcttcaat gctggaacag tggagtcgct tggaaagtcc gaagaaaacc tttcctcaac
     1921 aggcaccgtt gtttttgccg attgcgtcgg gaagataatc ttcataaaga cccagaaggg
     1981 cgttgaaagg ttcgagcttg tctcatccac aaagataaca agcggtgaca agccggcctc
     2041 ctgcgcagac atcaaggaag gccagcaggt aacaatcacc tatgaccgca agggaacaaa
     2101 ggttttcaca acaaaactca cgctcccgcc accaaagaca atcatcaaat tgactgtcgg
     2161 ccttggcgtc atatcggtca acggccagaa cgttctcatt gatgcaccac cttacgttaa
     2221 gggtggaaca acaatggtgc caatcagggt cgtaaccgca tattttggcg catcgctgac
     2281 ctggggccag gccgacaaaa ggctcacact ccagagggga aaggacatca tcatcgtctg
     2341 gatcggcagg caggaagcac ttgtcaacgg gaaatcaacc ccgctggccc aggccccgga
     2401 atacggtgca gccgacagga tgatggtccc gctaaggttc ttctccgagg tgtttggtgc
     2461 ccaggtggag tgggaccagg cgagcaagac gataacgata accctctagg aaatttgcgt
     2521 ctggaatatt caaaaaggag ggggaaaccc ctcctttttt gtttgtggcg gccacaaaaa
     2581 gaccctctca gctttgtttt tgctgttttc ttgccttcaa ggccgcaatt atctggccca
     2641 ttttttcctt gtccatggtt gtcatctgct cgaaatggtc tcttgtttcc tgcgatattg
     2701 caagtgtttt tccgaattcc ctgccggcgt ccctcatgag tgcataaaca tcggggaatg
     2761 ttgtttcatc gttgtctttg gccattccgt ctgcaaaggg aacaagaaaa tcctgggcat
     2821 aggagctgcc aaaaatctgc ttcattgtgg cctttagccc gtcgaagttt gacttttccg
     2881 ggagcccgca gggtgagatc aggacaaatt ttatcttttc aagccaggtc tttgagtcct
     2941 ttggggtgga tgaggtcgat ttgtggaaag tctccagata aaggggcaac tgcctgtcca
     3001 ggtagtcttt cacttttccg ggaacggtaa agaaataaag cggtacggag agcacaacga
     3061 gatcggcatt tctgtggtcc tcgacgattt ttgccatgcc atcgttttgg gcgcatttcc
     3121 cgggtgttgc agtccagcag cttttgcatc cggtgcaatg cccgatttca tagtcgttta
     3181 gcatgatttc ctggacctga tgacccacat cctccagccc cctggcaaaa tttgaaccaa
     3241 gcctgtacga ggcggaattt ttccctcttg gactgccata gacaactact gccttcattt
     3301 tgcctccttg tttgtagttg ctctagcata aatttttgtg cttatcaagt tctggctttg
     3361 aaccagcaat tgacagacat ttgcattgct tttctacgaa acgctgccat caaggtgcaa
     3421 ataatcccaa cttgcgccca actggtactt aatagtagac tcatcaaaga ggaggaggtt
     3481 gggaatgaag aaatgctttt ccttgttttt attgatgtca ttgctcatct ttccactggc
     3541 aaccggcaca tcaaaaacat cggactttgg tgttgcaacc agaagccggg ggaaactgga
     3601 tttcaaatca aggagccctt atgttttgtt cacttgccag agggaaggat tttttgaggt
     3661 aaggtcgttg caaacaggga aaagtgtttt ttgccatggc tgggttgatt acagccatgg
     3721 gccctttctc gaagatggaa aaataacttt ttatgatcca gacaagggtg ccaaaagcaa
     3781 tatgcgcatg gattttgacg gaaacgtgga gtattttgac cccacaatca accagaccaa
     3841 tccaccaacc agacacaaga ttgtccagaa cgccgacaaa tcctatactc ttagagtttt
     3901 atctgtagat ggcgataaag aaatctggac aatgactaca aaatcgggag agaaaattga
     3961 atacaaagat ggctggtttt tgtattctgg ctcagggttt atgaaggtgc tcaacccgat
     4021 taccggggag attgcccttg ctctggaggg caagactgtc cagtttgcag gacaaaacga
     4081 tgattttggt gttttctatg tttttgatgg cccaaaggat ttcgtttgcc agattctgga
     4141 ctggaaaacc ctgaaattgc tggtaaaaac aaaaatcccc tcggctgtga ctccaatatt
     4201 tggaaatggc gaagttattt tcgtttgtag aatagacagg cggttacctg atgccataaa
     4261 aatagagtca aactggggct ggacaagata caccagagaa aagggcattg aaaatttctc
     4321 tttttcagtc gagggtgtgt ttgaaccggc aacaaatgaa gtgattgatg caaaaggtta
     4381 tctcttgtca tttgccacaa ttgatccgca gaagcttgtg gtcgttgatg caagaatcgg
     4441 aaaggccgtt tttgagaggc cgttttaccc ggaaattgcc aatttttttg gcgatgttct
     4501 ttactaccag tcctggtttg attgtggcgc agtggatacg gccacctgga gaacgctctg
     4561 gagatttggt tttggcgggg aagccggaga gtacgatggc tacaagtact atgtccagaa
     4621 catggttttt ggggaaaaag atgaggggcc acttctccgg tttgaagtta tcgatctgaa
     4681 aaccggactc ctggagcctt atgaatatct ggttccatac aactggtcgg agattgtttt
     4741 tacaaagcac ggaatcgtca ccttgccaac tgaacagagc ttttcaaggg gtaccaggcc
     4801 aggctttgcg ctttttgccc ctggccttgc cgaacccatt tattcattgc acaagaattt
     4861 gggcggatgg gtaaagcact atgaaataac tgacaatgga gaatttttgt tgttggaggt
     4921 ggtgacccca tcaacaggcg agactcgcag atacaagctt gctattgcag atggttccct
     4981 tgaatttttg aagtgatcga agcttaaggg tttatggaac taattgtttt tgggtttgtc
     5041 atttttcttc cagatattca tacactctgt ggtgcagcgt cacctgtgcc cggttcctga
     5101 aagcttcggc aacaccaaac gaccagacag agttttttga gtatagcatg caattctcgc
     5161 ccttggcgtc aaagagtgcc gtctcgacct cctctggcgt gtcttcaaaa tttgtccttg
     5221 aaaggtaatg gattttgctt gtgaagtagt caaaaattgt tgctttgtag gccaaaactt
     5281 tgtgttttcc gagagattca taactgacaa tgaaaattgc attttctccg ccgttttcat
     5341 tgaaactcgt acaagtcagc aattttgcat tttttgtctg gctgatgccg tcatatgcga
     5401 aatatgcagc tttttggggc acttgctgtt tcccgccaaa ataggacaca cagccgttca
     5461 caaaaaacag gccaccgtca aaaggaaaga gcatgtttgc caggccgggg cgcacaccgt
     5521 catgttttat tgagaatatg aacctggatt ccctctggtc gaacaaaaac aatttttggc
     5581 cgtcatagat tgagatgatt cccctgtctg ggtttttggt gtttccgcaa atgaaggatt
     5641 tttcgatgtc tcccttggtc ccaagcggga ttttcttgta aggggttatt ttttctttga
     5701 acacggtgta aaaaatcagg tttgtgccgt catagccaat aagaccactg gaaccatttt
     5761 tgtaattttc cgcaaaaatg gttttgggtt caaatgggta tgatgccagc tcgccggtgt
     5821 tgctgtaatg gaaaacttcc cctcttttgt ttgtgcagag aatgattgtc ttgttgtcgt
     5881 caaatggcag gtaccggcca attattgtgt ctgcgtatgt ctccacataa aaagcgttct
     5941 cgaaatgctt gtagtagtca gaaaaaaccg tgtggacatt ttcaacctta tagagaacaa
     6001 gagcattgct cgtgtcactt tcaaagatgg ccgcaatgtt ggttgtgctg gtgtcatgac
     6061 tgcctggaat tgggtctttc ttgatattta tgcttagaag tttaccaggc gactctatat
     6121 tggtcagcgt ttctttgaga ttttccgtta gaatgtacag gccgcaatct gatttgtcaa
     6181 agacaaccgg caaatacccc ttgctcgtgt ctttggggtc ctcgtacaga tagccgacga
     6241 tttctctgtc ggtgttgctt gcaaccagct tgagtttggc gcgtcccgga tcaagaatta
     6301 tcgcggccaa aaccagcgag ctggtcacca gaattattac cacccagtaa atcaaatttt
     6361 tgtttatccc tgcccctgtt tgcccagctt cgctcatttt ttgcgcctcc agctgtttgt
     6421 tgatctatcc aaaacaccgt atatcctatt tgacacccga agtgatggtt atttttttgg
     6481 tcttctggtc atagtcaagt ttgcacccga ggttttcggc aaggaacctc attgggacaa
     6541 gcgtccttcc accagagatt attggcacca ccttggggtt cttggggtct atctggatct
     6601 cttttatgtt gacagtagct ttttcatgac caatcctcat tctgatcgtg ttgccattca
     6661 atttaactgt aacttctttc catttatcat tccatagcgt gtcgccaccc atcggttcgg
     6721 ccacaaattt cgcaggcagg tatgtgattc ccccgattgt ctgaagcttt actcccatgt
     6781 cctgctccgc tccatcagcc ttgaatttgc tgctgccaat ctggaactcc agcttttttg
     6841 ctgcaccttc ggaaaagcaa tagagataat ttggtttgtt gaccacgaag agataatctt
     6901 ttatcggcac gacgaaagat ctgcggttga tgtaggattt tttattgtca ctgctgccat
     6961 caaaacagaa atcaaagctt cctgtttttg tatttgtcaa agtgttgaat atttcgaggt
     7021 tactactttc aaagaaggaa aaatccaggg aagatgaatt gcttgcgtaa gcgtagtccc
     7081 cggaccttgc gtagcaatcc atataggact tgtcttccca gatgcatccc ccggtctcta
     7141 tgtcaatgaa catgtttcta cacctgagct tgccatcaga aaatgggttt tcatcatagt
     7201 taagtggcag tgagtagagt tttttaccgt tggatgcatc aaaacagcat aagccctccc
     7261 aatgataata gtaagtatgg cattcaattt gtgcacacac aatgttattt tttataaaaa
     7321 tattgccagc tttcatatat cttgatgtgc cgtaaagagc ctgatttgtt tcatctggat
     7381 caaaaaatct ttgctcccag atcagcttac cacttgtaac atcaagacat gacagtatta
     7441 atgagatacc ttcatctgga tatccatcat caatgttttt cttgccttct ggtcggcata
     7501 aatatactct gtttgagtca aacgctattt ggaaaataag atctccgtca ctattattgt
     7561 aatttttgct ttccttgtgt tttgtctg
//
LOCUS       DAOSRI010000045         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9100_length_7571_cov_3.247339, whole genome shotgun sequence.
ACCESSION   DAOSRI010000045 DAOSRI010000000
VERSION     DAOSRI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9100_length_7571_cov_3.247339"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK717_02260"
     CDS             complement(<1..214)
                     /locus_tag="PK717_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR46520.1"
                     /translation="MGKILALSNQKGGVGKTTTAVNLGYALAKRGVKCLICDIDPQGN
                     ATTGVGVDRMDVKKSTYDLLIGYGTLD"
     gene            complement(229..574)
                     /gene="ssrA"
                     /locus_tag="PK717_02265"
     tmRNA           complement(229..574)
                     /gene="ssrA"
                     /locus_tag="PK717_02265"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1080..2639
                     /gene="hutH"
                     /locus_tag="PK717_02270"
     CDS             1080..2639
                     /gene="hutH"
                     /locus_tag="PK717_02270"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOR46521.1"
                     /translation="MRNINLGENLDFRSFLDLSHGHAKSAIGKESMEKMSDCRKMVDK
                     VTRGDAAVYGLNTGFGSLARVKIPSGDLEKLQENLIRSHSCAVGEPLPMDVVRGAMIL
                     RANTLCLGHSGIRPEVVELLVQMVNKEIIPKVPLYGSVGASGDLALLSTIACCLIDNS
                     DPEFMVFCHENGSWTEKHSRKALDDAGLKPVKLTSKEGLALNNGCQVSTSLGLLAIER
                     GILSLQAWDIALATSAQALLAKSSPFDERVSIARPHKGHKKQAEIIRNLLKDSALIDF
                     DKEKVQDAYSVRCAPQVGGAVRDAIAFALEQLSIEMNSVTDNPLIYGDVLSGGNFHGA
                     PVAQACDLLCMSFTDLGSMSERRTFRLINSHLSGLPSFLTDNPGLSSGFMIPQYTAAS
                     LVSACKALSHPACVDSIPTCEDQEDHVSMAPISAWKLWKIVDNLSWIVSIELLTAFQA
                     LEMRLSSLGLSINDISYPTRTILGKVRQKVPYIQADQPLSSKIGLMRDMVLDKTLINP
                     ISDIWSSDVVD"
     gene            complement(3035..4324)
                     /gene="hisS"
                     /locus_tag="PK717_02275"
     CDS             complement(3035..4324)
                     /gene="hisS"
                     /locus_tag="PK717_02275"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOR46522.1"
                     /translation="MKAPQILSGFQDLLPGDMIARNKVMSMIKAVFESHGFEPIETPS
                     LEYYETLTGKEGGESEMLMYDFTDHGDRHVGLIYDLTVPISRVMATYQDIPKPFKRYQ
                     IQRVYRAERPQKGRFREFYQCDVDIFGTASPLSDAEIVSILSDSLFRMGFKDFNILVN
                     HRGVMQGIMRACGVPKEILKESIIIVDKLDKVGPDGVAKELEAKGIPSAGRIVEMISV
                     QGDTSNVISKLKGMLASDGEAVQALDNIRQIIELAGAMSARVKFDPSLARGQSYYTGP
                     VFEARLETPKIGSVAGGGRYDNLIGIFSGNQVCATGASLGIDRIMAAMREIGMISDTK
                     TIAKIQICHFGEGSLSLALECAKTIRESGIPCDLYCGDGDIRKQIKYASKKGVPYIVI
                     IGEDEAKSSTVLIKNMETGEQEKVEFSKLGEVASQLV"
     gene            complement(4324..4893)
                     /locus_tag="PK717_02280"
     CDS             complement(4324..4893)
                     /locus_tag="PK717_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOR46523.1"
                     /translation="MQEKKGEVMNTGTVGNMWEKWNIAGGYGIRPACKIFRINMLPGD
                     ETKDMEALNDFLSSRTILLVQPQFVPSKVDHWLVFVIYYDVEHGRQNGGNPQKTEEIL
                     DERAETPSKLDYDESLAEKLRAWRSKEAKSRGWPAYIILYNKTIEDIARKMPTSLENL
                     LQVEGANKKTIEEFSSEILGIVREHIKEM"
     gene            complement(5065..6360)
                     /gene="dnaA"
                     /locus_tag="PK717_02285"
     CDS             complement(5065..6360)
                     /gene="dnaA"
                     /locus_tag="PK717_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOR46524.1"
                     /translation="MNIWQQVLQVIQQDVTKPTFEMWFATSELLSETYDRLVVSVPSE
                     FAKDWLQSKYYNTIQNILHDLTGKDIDITFVVQRSMEHVPEMEEPQTNDHTLNPKYTF
                     SSFVVGNSNKFAHAACLAAARDPGRAYNPLFIYGGVGLGKTHLLQAIGHEILARKPAA
                     DLIYTTTEKFTNEVVYAIQQNRIPEFHEYYRTVEVLLIDDIQFLTGKERTQEEFFHAF
                     NALYDRSRQIVVTSDRPPKELQGIADRLRSRFEMGLLADIQPPDLETRIAILQKKASL
                     EHVDIPQEVINYVAGHSFSNIRELEGSLVRLVAVASLQNRELTLEVAREVLRDIVPKG
                     NRRITITRIMEEVARFFKLDVKDLTARRRTHDIAIPRQIAMYLAREYTDDSLIAIGEQ
                     FGGRDHTTVMYAYDKITQERRSDQTLDAAIHEILSILER"
     gene            6804..>7571
                     /locus_tag="PK717_02290"
     CDS             6804..>7571
                     /locus_tag="PK717_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46525.1"
                     /translation="MKKTISILIATLMLMTVLPVFGNASMARAENSNANGVFVAVRFE
                     ASGWRFSGNSHVTMPNQEIIWGRPPQYPMPANIHAWTEFYLDVVPAAGMSTESNHWYA
                     VIDTDGNLWFDSDGNFHDCRYYKYADPEDPDYGTDAASTIDNCQTNPHARFDPTSANN
                     TQGPYQVPRGNNISGAIGAITPFSKSANPTYFMDPKTGRTFRIGIADLVARNSGVVGT
                     SWINPATGNPLVSNPAPEWDQGMALERFLDGYGPGAPP"
BASE COUNT         1864 a         1831 c         1675 g         2201 t
ORIGIN      
        1 catcgagtgt gccatagcct atcaaaagat cataagtgga ctttttaaca tccatccggt
       61 caacaccaac accggttgtt gcgttgcctt ggggatcaat atcgcaaata aggcacttta
      121 cgcccctttt tgcaagagca tagccgaggt ttacggcggt agttgttttt ccaactccgc
      181 ccttctggtt tgaaagcgca aggatcttgc ccatttttca cctttttttg gtggaggtga
      241 cgggattcga acccgtgtcc aacttgcctt tcgaaagaca gggattacaa gctttgcaag
      301 ccgtttaccc tcttagccta tggcttgccc tttggcagag ggttgcccag attgtaccat
      361 gggccatccg cccgggcggc agatgacttg ggggcacttg tgcgtcggct tccggttacc
      421 agcgccactc tcaccggttg ccgtgtcgcc taattaggcg gcgagtgcgt aattatttgt
      481 ggcagttatg ccgtggtcca ttttttacga ggccctggac caacctcggc ttgcctgttc
      541 cttcaattgt caagtgtcga gacctttcac ccccatgatg ttattattgg cttttgcaca
      601 aaaaagttca ttcatgctat tgtgaaacgc cgtgaaacat tttataccag taatgttcat
      661 aatgtcaagg aggcttggtt ttttcaaatt cttatcaaca tatccacaaa atgtgtgaaa
      721 cattgtgaaa catttttcat ccggtctcca aatggatgtc cgcataaagt ttttctccac
      781 gtgccccaaa acaacctagt aaactgttca caattttatc cagaatcatg caaatacttt
      841 taggttgcct ggcagcttgt tttgagtaat ggtcaaaaat actctcctaa aaaggcgtga
      901 aacactgtga aacattttga ctgactttga tgatattggc gtgaaattct ttctaataca
      961 tattttttta aaaattatgg atttagtccg tgaaacaccg tgaaacatta actgccggca
     1021 aaccggtgtt tcattgacaa aaccaatcta atccctaaac cttttcgttg gaggtaacct
     1081 tgagaaacat caatcttggt gaaaatcttg atttcaggtc tttccttgac ctgtcacacg
     1141 gacacgcaaa atcagcaatt ggcaaggaat ccatggaaaa gatgtccgat tgcagaaaaa
     1201 tggttgataa ggttaccagg ggagacgcgg cagtttacgg cctcaatacc ggttttggga
     1261 gtcttgcaag ggtaaaaatt ccatcaggcg acctggagaa actccaggag aacctcattc
     1321 gctcccattc atgcgcggtg ggcgagccac ttccgatgga cgttgtcagg ggcgcaatga
     1381 tcctgagggc aaacacgctt tgcctcgggc attccggcat aaggccagag gttgtcgagc
     1441 tgctcgtgca gatggtcaat aaggagatca tacccaaagt cccgctttat ggctcggttg
     1501 gtgcctctgg cgacctcgca cttctctcca ccattgcttg ttgcctcatc gacaacagcg
     1561 acccggaatt catggtcttc tgccatgaaa atggctcctg gacagaaaaa cattccagga
     1621 aagcccttga tgatgccgga ctaaaaccag tcaaacttac atcaaaggaa ggtctggcac
     1681 tcaacaatgg ctgccaggtt tctacatcgc ttggcttgct tgccatagag aggggtatac
     1741 tctcgcttca ggcatgggac atagcccttg ccacatcagc ccaggccttg cttgcaaaat
     1801 caagcccttt tgacgagcgt gtctcgattg caaggcccca caaggggcac aagaagcagg
     1861 ccgaaatcat aagaaatcta ctcaaggatt cggctctcat tgactttgac aaagaaaaag
     1921 tgcaggacgc atattcagta aggtgtgctc cacaggttgg tggcgctgta agggacgcaa
     1981 ttgcttttgc acttgaacag ctctcgatag agatgaactc ggtgaccgac aatccgctca
     2041 tctatggtga tgtgctctcc ggtggaaact tccacggagc accggttgcc caggcctgcg
     2101 atctcctttg catgtcgttt acggaccttg gttcgatgtc cgaaaggcgc acgttcagac
     2161 tcatcaattc tcatttatca ggccttccat cattcctcac cgacaaccct ggcctttcat
     2221 caggcttcat gattccacaa tacaccgcag cttcgcttgt ttctgcatgc aaagcacttt
     2281 cacatccagc ttgtgtcgat tccattccca cctgcgagga ccaggaggat catgtttcca
     2341 tggccccgat ttccgcctgg aaactgtgga agattgtgga caacctgtca tggattgtga
     2401 gcattgaatt gcttacagcc ttccaggccc ttgaaatgag gctttcctcg cttggattgt
     2461 ccattaatga tatttcatat ccaacaagga ccattcttgg taaagtaagg cagaaagttc
     2521 catatataca ggctgatcag ccactgtcat caaaaattgg cctcatgcgt gacatggttt
     2581 tagataaaac actcatcaat ccaatatctg atatttggag cagcgatgtt gtggattaat
     2641 tttgtggata tgtggaaact ttaccgaaac cttggatgca agattctttc gattatgtaa
     2701 ataagtgtta aacaatcata agtgaaattt tgccatatat ttacaaatta cataggttgt
     2761 tttttgattt caaatgtttg atatttactg ctgaaaccta tttttttgtc gatttacttc
     2821 aaatttccag atgaaaatgg caaaactaaa ttttccccca catcgcatag aaatcagttt
     2881 ttaaatcagt gtttcgaacc atataacatt tttaacattt atccacttat tttccaaagc
     2941 atgttccgac aatagttctt gccggtttaa aaaataaccg gtgttataaa ttatctggaa
     3001 caaactggag agccatcagg gttgtttaaa gctctcagac cagctggctg gcaacctccc
     3061 caagctttga aaattcaacc ttctcctgtt ctcctgtttc catatttttt atcagtactg
     3121 tcgatgattt tgcctcgtct tcgccgatta ttacaatgta tgggacaccc tttttcgagg
     3181 catattttat ctgtttcctg atgtcgccat cgccacaata tagatcacac ggaatacctg
     3241 attcacggat ggtttttgca cattcaagtg caagggatag ggaaccttcg ccaaagtggc
     3301 agatttggat ttttgctatg gtctttgtgt cacttatcat gcctatttcc ctcatggccg
     3361 ccatgatcct gtctattcca agcgaagccc cggttgcaca cacctggttc ccggagaata
     3421 tgcctatcag gttgtcatac ctgccaccgc cagcaactga gccgatcttt ggcgtctcaa
     3481 gccttgcttc aaaaaccggg ccagtgtagt atgattgccc tcttgcaagg gatgggtcaa
     3541 atttcaccct tgcactcatt gcaccggcaa gctcaatgat ttgtctgatg ttgtccagcg
     3601 cctggacggc ctccccatca ctagcaagca ttcctttaag ttttgaaatc acattgctgg
     3661 tgtcgccctg gacagatatc atctccacta ttctcccagc cgacggtata ccctttgcct
     3721 caagctcttt ggccactcca tctggcccca ccttgtcaag tttgtccaca atgattatcg
     3781 attctttgag tatttccttg ggtacaccac aagctctcat tattccttgc attactcccc
     3841 tgtgattgac cagaatgttg aaatccttga aacccattct gaaaagagaa tcagacagta
     3901 tcgaaactat ttccgcgtca gaaagtggcg aggcggtccc aaaaatgtcc acgtcgcact
     3961 ggtagaattc cctgaacctt cccttttgcg ggcgttcggc cctatagacg cgttgtatct
     4021 ggtacctctt gaacggtttt ggtatgtcct ggtaagttgc cattaccctc gagatcggaa
     4081 cagtcaaatc atagatgagt ccaacatgtc tatcgccatg gtcagtgaaa tcgtacatga
     4141 gcatctcgga ttcgccaccc tcttttccgg taagcgtttc atagtactca agggaagggg
     4201 tctcgatagg ttcaaaacca tgggactcga aaacggcttt tatcatggac atcaccttgt
     4261 tacgggctat catgtcgcca ggaagcaagt cttggaaacc tgacaaaatc tgtggtgctt
     4321 tcattacatc tccttgatgt gctctctgac tatccctaat atctcggagc tgaattcttc
     4381 tattgtcttt ttgttagcac cctcaacctg caggaggttt tcgaggcttg ttggcatttt
     4441 tcttgctatg tcttctatcg tcttattata gaggataatg taggctggcc atcctctgct
     4501 cttcgcctct tttgatcgcc aggccctgag cttctccgcc aggctttcgt catagtccag
     4561 ctttgatggc gtttcagctc tttcgtctag gatctcctca gttttttgtg ggttgccgcc
     4621 attttgtcta ccgtgctcaa cgtcgtaata aataacgaac acaagccagt ggtctacctt
     4681 tgatggtaca aactgtggtt gtaccaaaag aatagtccta gaggacaaga aatcattcag
     4741 tgcttccatg tcttttgttt cgtctcctgg aagcatgttt attctaaata tcttgcaggc
     4801 aggccttata ccatagcctc ccgcaatgtt ccacttttcc cacatgttcc caactgtccc
     4861 cgtattcata acctcccctt tcttctcctg catttcttcc aattttccac agtgttaaca
     4921 cctttattat gaattatgtt cttggatgca aaaacttcat aatcataata agtatgttga
     4981 taaggtgcat aagttgcctt atgcaacaaa cctactgcaa attgtcctaa gaaaaatttt
     5041 ctcataactt gtctaaagtg tggattacct ttcaagaatg gaaaggatct catgtatcgc
     5101 agcatcaagc gtctggtccg accgcctctc ctgagtgatt ttgtcatagg cgtacattac
     5161 ggtggtgtga tccctgcccc caaactgctc accaatggct atgaggctgt catctgtata
     5221 ctctcttgca agatacatgg ctatttgcct tggtatggct atatcgtgag tgcgcctgcg
     5281 tgcagtgagg tccttgacgt caagtttgaa gaatcttgca acttcctcca tgatccttgt
     5341 tatagttatt ctgcggtttc cctttgggac aatgtctctt aacacctctc ttgcaacctc
     5401 aagggtcagt tccctgttct ggagtgaagc caccgcaacc agcctgacga gcgacccctc
     5461 gagctctcgt atgttcgaga agctgtgccc cgcgacataa ttgattactt cctgggggat
     5521 atccacatgc tctaaactgg ccttcttctg gaggattgct atcctcgttt cgaggtcagg
     5581 tggctggatg tctgcaagga gacccatttc aaacctggat cttaatctgt ccgcaatccc
     5641 ctggagttct ttgggcggcc tgtccgaggt tacaactatc tgacggctcc tgtcataaag
     5701 ggcattgaat gcgtggaaga actcttcctg agtcctttcc ttgccggtga ggaactggat
     5761 gtcatcaatg agcagtactt cgacggttct gtagtactcg tgaaactctg gtatcctgtt
     5821 ttgctggatc gcatagacca cttcgtttgt aaacttctct gtcgtcgtat agataagatc
     5881 tgcggcgggt tttcttgcca gaatctcatg ccctattgct tgtaaaaggt gggttttccc
     5941 caaaccgacg ccaccgtata tgaagagtgg gttgtaagcc cttcccgggt ccctggcggc
     6001 agcaagacag gccgcatggg caaacttgtt tgaattgccc acgacaaagg acgaaaaggt
     6061 atacttgggg tttagggtat ggtcgtttgt ttgcggttct tccatttctg gaacgtgttc
     6121 catgcttctc tgcaccacaa acgtgatatc aatgtccttg ccggtcaggt catgaaggat
     6181 gttttggatg gtgttgtaat attttgactg gagccaatcc ttagcgaatt cggaggggac
     6241 agagacaacc aatctgtcgt aggtctccga gaggagctcg gaagttgcaa accacatttc
     6301 aaaagttggt tttgttacgt cctgctgtat aacctgaagg acctgttgcc aaatgttcat
     6361 gtggattata ttagcaaacc agatggcccg gtcaacgtta accttatata tacacttttg
     6421 taacctttat atccccattg tgttgcattc aaccctcaag tgcgatatag tctcaaataa
     6481 taccgtatca aatgatttaa atggcaggac ccatttgtcc ggtcagcgag aaaaaggcct
     6541 aaaaatgcaa cacatcaacc gtggcatggg aattgcatga taacaaaacg gtgatgatca
     6601 ataggtgtga gaaaacatct tttgattgtc atgttgtttt tataaggagt tggatttata
     6661 tggtgctttg atgaagtcac agattcatgg tgtggcttcg tttggttttg cgcactttga
     6721 ttcattcctt gtgcgtccat gcgttggtgg ctttgcgaac gccgcaagcc gcttttgcaa
     6781 caaattgaaa ggagagtttg gtgatgaaaa agacgatcag cattctgatt gcgactctca
     6841 tgctgatgac ggttctgccc gtctttggca atgcctcaat ggctagagcc gagaactcta
     6901 atgccaatgg tgttttcgtg gctgtgagat tcgaagcttc cggttggaga ttcagcggta
     6961 atagccatgt gactatgcca aaccaggaaa tcatctgggg aagaccaccg cagtatccaa
     7021 tgccggccaa tatccatgcc tggaccgagt tttatctcga tgtagttcca gctgctggca
     7081 tgtcaactga gtctaatcac tggtatgcag taattgacac agatggcaac ctctggtttg
     7141 actcggacgg aaacttccat gactgcaggt attataaata tgcagacccg gaagacccgg
     7201 attacggaac cgacgcagcc tcaacaatag ataactgcca aacaaacccg catgccaggt
     7261 ttgacccgac ctctgccaat aatacacagg gaccatatca ggtaccgagg ggcaacaaca
     7321 tctccggtgc catcggtgcc atcaccccat tcagtaaaag tgccaacccg acttatttta
     7381 tggacccaaa gactggcaga acgttcagga ttggaatagc agaccttgtt gcccgtaact
     7441 cgggtgtggt aggcacttct tggatcaatc cggcaactgg aaacccattg gtcagcaacc
     7501 cagcaccaga gtgggaccag ggaatggccc tggagcgttt ccttgatggt tatggtcctg
     7561 gtgcaccgcc g
//
LOCUS       DAOSRI010000046         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9113_length_7565_cov_4.519441, whole genome shotgun sequence.
ACCESSION   DAOSRI010000046 DAOSRI010000000
VERSION     DAOSRI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9113_length_7565_cov_4.519441"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1270
                     /locus_tag="PK717_02295"
     CDS             80..1270
                     /locus_tag="PK717_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOR46526.1"
                     /translation="MKKFFAFMILIPIAFASCGKASIQISNEPDWNTLRMSILRASIS
                     NHSNLGQFGEYVSNAGLTSSDLEKYLDENGSLKSNSPESLAKILQKLDNDRDAIINIA
                     KDSITFANKLGGRAEKIANFAKTTLPFENITVQADPSLSPGLTRVSIAEKTAILFVPP
                     MAGKESFDSALLNAILQNAVLGKTYNIESIEKMCQKYSASLSPREQVFADLAFSLSTW
                     IDNPDIPTDLRAYTLKSSLKYGHIFSPQLGYGDMSVMENTKLEEIVDQAFADCSNPKY
                     LTLLESLLNPGRLGIKVKEIPEGLEINELSLSGPSEQAGLLPGDIIVEIDTQKITKVW
                     ELENLLYDKAANQYVIVKVSRDKTFSREKATTFEDDPSNSSRLCLFYQLLVAPKNSLP
                     EGVY"
     gene            complement(1264..1959)
                     /locus_tag="PK717_02300"
     CDS             complement(1264..1959)
                     /locus_tag="PK717_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOR46527.1"
                     /translation="MPKGQILIVDFGTRHLNSLVLVVKSFIDTENIRVLRITRPDLPP
                     LSFKFTDNIETEESPWEFNVDSIRGTDMGVFMSGSADHVGDPKGFRRLNKNVLEELPC
                     PVMGLCYGHQLISFVWDDSCEMVPHSEHGNTMFLPSTEMSHDPVFQNMPKAGFLVAVR
                     HNWSISKVPTGFVRYGETRGSHSIISAIKHYNRPIYGVQFHPELPVPGLWSGRELMGK
                     FIELSISGALTPQ"
     gene            complement(2090..2458)
                     /gene="rplL"
                     /locus_tag="PK717_02305"
     CDS             complement(2090..2458)
                     /gene="rplL"
                     /locus_tag="PK717_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOR46528.1"
                     /translation="MNKDEIIAAIEKMTVMELNELVKAIEEKFGVSAAAPVMMGVMPG
                     AGPAADVEQTEFDVILTEAGQGKIGVIKVVKEITGLGLKESKELVDAAPKPVKTKIPR
                     AEAEEIKEKLAAAGAVVEIK"
     gene            complement(2488..2985)
                     /gene="rplJ"
                     /locus_tag="PK717_02310"
     CDS             complement(2488..2985)
                     /gene="rplJ"
                     /locus_tag="PK717_02310"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR46529.1"
                     /translation="MVTKQKKNELLEELREQWKNSVGLIFSDTQLLPSTAVAELRREV
                     RKANLFHKVYKNTLIEKVAKEMGVTGLEKYLFGPTAITFAKEEAPVAAKVIADLAKKF
                     PKKLALKAALTEGQVYDSAGANALANVPSRQQLLGMIAYVLIAPVGSFARAVNAVKEQ
                     KEKAA"
     misc_feature    complement(2995..3140)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3151..3858)
                     /gene="rplA"
                     /locus_tag="PK717_02315"
     CDS             complement(3151..3858)
                     /gene="rplA"
                     /locus_tag="PK717_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR46530.1"
                     /translation="MFRGKRYKKLEKVFDKTKLYDVSEAIDILKKLKSTKFDETIEVG
                     LKLGVDPKQADQYIRGTVSLPAGTGKTVRVLVFAKGEKATEAKEAGADYVGDDDIIEK
                     IKGGWFDFDLAIATPDMMGSVGKLGKILGTKGLMPNPKAGTVTMEVDKAVKEFKAGRI
                     EYRISKFADMHVPVGKISFSNEDIQKNILAFLSAIVRAKPASLKGTYIFTMTLSLTMS
                     PGVKLDVSKATAAAIKA"
     gene            complement(3911..4342)
                     /gene="rplK"
                     /locus_tag="PK717_02320"
     CDS             complement(3911..4342)
                     /gene="rplK"
                     /locus_tag="PK717_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR46531.1"
                     /translation="MAVKKKITGYVKLQIEAGKANPAPPVGPALGQRGVNIMQFCKDF
                     NEKTKDKPGMIIPVIISVYEDRSFDFILKTPPVSDLIRKALKVPAGSGEPNKKKVGVL
                     TMSQVEEIAKVKMPDVNAFDIEAAKRMVMGTARSMGVDIGK"
     gene            complement(4355..4891)
                     /gene="nusG"
                     /locus_tag="PK717_02325"
     CDS             complement(4355..4891)
                     /gene="nusG"
                     /locus_tag="PK717_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR46532.1"
                     /translation="MSSLPEPHWYILHTYSGYENKVKINLIQRVQSMNMEDLIFRIEV
                     PVDYQVKLKDGKRQVLPEKVYPGYVLVEMIMTDQSWYVVRNTPGVIGFVGSGGKPLPL
                     EEEEVQQMLKRVGLEEAKAALNFSVGDTIRVVGGPFNEMIGIVEAINEEKEKAKVRLS
                     MFGREMPVELDFVNIEVI"
     gene            complement(4895..5281)
                     /gene="secE"
                     /locus_tag="PK717_02330"
     CDS             complement(4895..5281)
                     /gene="secE"
                     /locus_tag="PK717_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOR46533.1"
                     /translation="MNKLRTNFFKDMWYEMTNKVTWPTWKRLVKATTIVIVFIMIWAV
                     ILYAFDSVFSSFQSWLAQDYYDPVALQNGEVKKFESQQKLDEWMESQKKAGVKGYVPE
                     NQENTGTPTPGNGGQTTPPSNEGGNK"
     gene            complement(5293..5368)
                     /locus_tag="PK717_02335"
     tRNA            complement(5293..5368)
                     /locus_tag="PK717_02335"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5333..5335),aa:Trp,seq:cca)
     gene            5515..6477
                     /locus_tag="PK717_02340"
     CDS             5515..6477
                     /locus_tag="PK717_02340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR46534.1"
                     /translation="MQRATLFFNKIQLEDADKLIKKQAKTRLVTLLTVTTLILSGLTA
                     AFFVRFPAQNTQPVAQDVSKQDNFEKKMFSIGEQIVKSTTPCKEPQKAEPMTISPQKI
                     AAKPLESAPKTQPKSVPTKIAQSTKTPARTITKTPTTTKPKTTTQAAAKTTQKTAQGG
                     DNLPVKTRDLGKIDESLPAAKLDPMPVNANFGIPEYWPLPVKVSDPYGYLPWRGRVHA
                     GIDLTAPCDSLILAAGNGKVIRAEWYAGYGKCIDIQHASGYITRYAHLTDYLVKVGDF
                     VEAGEWIGKVGSTGNSSCCHLHFEVHLNGRTIDPESIHWLNRTY"
     gene            6497..6958
                     /locus_tag="PK717_02345"
     CDS             6497..6958
                     /locus_tag="PK717_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46535.1"
                     /translation="MQIDFSDKEFFRQLGATLNNATWELIDKDERSSEESMKMIEMAH
                     ASCYFWTEFGGPLNVQRGHWLVSRAYACVKLPWPCLHHAKICMEITEKQGLKDFDLAF
                     AFEAMARAYSISGNEAEKANYLEKAKRVGEQIKDSEDKNIFFQELDRGPWV"
     gene            complement(7060..7515)
                     /locus_tag="PK717_02350"
     CDS             complement(7060..7515)
                     /locus_tag="PK717_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOR46536.1"
                     /translation="MTGQDAIKFFHWTRSRTIKILQSLFVETLDYKLPSKKPRKDSPG
                     KIRRILKHIVQAEVGWIHRLVMKEKEAENYDISTMGIEEIIALLERTRAKTVKWLEEN
                     KDKPLEFPYGKKASTHVAWIMYHVAEHEAYHLGQIVLIAEMNGQQIPWI"
BASE COUNT         2049 a         1772 c         1533 g         2211 t
ORIGIN      
        1 acgaaagacc gggtgggttc atgtataatt atccaaaccc gcctgatctt gatgaagctc
       61 tttcggtatt ggaggaacaa tgaaaaagtt ttttgctttt atgatattga tcccaattgc
      121 atttgcctca tgcggcaagg catcaataca gataagcaac gaaccagact ggaacaccct
      181 tagaatgtca atcttgagag catcaatcag caaccattcg aaccttggcc agtttggaga
      241 gtatgtttca aatgctggtt tgacaagctc tgacctcgaa aaatatcttg atgaaaatgg
      301 ctccttgaaa tccaattcac cagagagcct tgccaaaata ttgcagaagc tcgacaacga
      361 tcgcgatgca ataatcaata ttgccaaaga ttcaataact tttgcaaaca agcttggtgg
      421 aagggcagag aaaattgcca attttgcaaa aaccaccctt ccctttgaaa acatcaccgt
      481 gcaagccgat ccctcattgt cacctggcct tacaagagtg agtattgcag aaaaaactgc
      541 aatacttttt gtgcctccaa tggccggaaa ggagtcattt gattccgccc ttctgaatgc
      601 aatcctgcaa aatgctgttc tgggaaaaac atacaatata gaatcgattg agaagatgtg
      661 tcagaaatac agcgcatctc tttctccaag agagcaagtt tttgctgatc tcgctttttc
      721 gctgtctaca tggatcgata acccggatat accaacagac ctccgagcat acactttgaa
      781 atcaagcttg aaatatgggc acatattttc cccacagctt ggctatggtg acatgtcggt
      841 aatggaaaat accaaactgg aggaaattgt tgatcaggcc tttgctgatt gctcaaaccc
      901 caaatatctc acattgcttg aatcactttt aaaccccggg agattgggaa taaaggttaa
      961 ggaaatccct gaaggtcttg agataaatga attgagtctt tctgggccat cagagcaggc
     1021 aggacttttg cctggagata taattgtaga aatagacact cagaaaatca caaaagtttg
     1081 ggaactcgaa aatcttttgt acgacaaagc agcgaaccag tatgttatcg tgaaagtatc
     1141 cagagacaaa acttttagta gagaaaaggc aacgactttt gaggatgatc cttcaaattc
     1201 ctcgagatta tgtcttttct accaattatt ggttgcccca aaaaattccc ttccggaagg
     1261 ggtctactga ggagtcaggg cccctgatat tgatagttcg ataaatttgc ccatcaattc
     1321 tcttccactc cagagacctg gaacaggaag ctccggatgg aactggacac cataaattgg
     1381 tctattgtaa tgttttattg cagaaataat tgaatgtgat cctcttgttt cgccatatct
     1441 aacaaacccg gttggcacct tggatattga ccaattatgt cttactgcaa caagaaaacc
     1501 tgcttttggc atattttgaa aaacaggatc atggctcatt tctgtgcttg ggaggaacat
     1561 tgtgtttcca tgttcggaat gaggcaccat ttcacagctg tcatcccaca caaaacttat
     1621 aagttggtga ccataacaaa gacccatgac agggcacggg agctcctcca gaacattctt
     1681 gttcaatctt ctgaaacctt ttgggtcacc aacatggtcg gcagatccgg acataaaaac
     1741 tcccatgtcc gtacccctga tcgaatcgac attaaactcc caaggggatt cttctgtttc
     1801 tatgttgtcg gtaaatttaa acgagagtgg cggcaaatcc ggccttgtta ttctgaggac
     1861 acgtatattt tcggtatcga taaagctttt gaccacaaga acaagcgagt tcaagtgtct
     1921 cgttccaaaa tcaacaatta gtatctggcc tttgggcata tttaaataat attaccttgt
     1981 ttcaatatga caagttttgc cattttacct gttttactga accaataaat gcaaaaactc
     2041 cggccaattt tttggccgga gttttggttt gtacgatgtt tttgtcttac tacttaatct
     2101 cgacaactgc acctgctgct gcgagctttt ccttgatctc ttcagcttct gctctcggga
     2161 tctttgtttt gactggcttc ggtgccgcat caacaagctc tttggattct ttgaggccaa
     2221 gtcctgtgat ctccttgacc accttgatga ctccaatttt tccctggcct gcttcggtaa
     2281 ggataacatc aaattcagtc tgctcaacat ctgcagctgg accagcgcct ggcattacac
     2341 ccatcattac tggggctgct gcagagacgc cgaacttctc ttcgatggcc tttacgagct
     2401 cattgagctc catgacggtc atcttttcaa ttgcggcgat gatttcgtct ttattcattc
     2461 ttgaactcct tttttatttg tttgttttta tgctgctttt tctttttgtt cttttactgc
     2521 attgactgct ctagcaaatg atccaacagg tgcaatcaag acataggcta tcatgccaag
     2581 gagctgctgc cttgatggga cattggccaa tgcgtttgct cctgcactgt cataaacttg
     2641 tccctcagtt aaggccgcct tcagagcgag cttcttgggg aacttctttg ctagatcagc
     2701 aatgactttc gccgctactg gagcctcttc ctttgcgaat gtaatcgcgg tcggcccaaa
     2761 aaggtatttt tcgagaccgg taacacccat ttcctttgca actttctcga tcagggtgtt
     2821 cttgtatacc ttgtgaaata aatttgcctt cctcacttct cttctcaact ctgcgacagc
     2881 tgtcgagggt agaagttgtg tgtcggagaa aataagacca accgagtttt tccactgttc
     2941 cctgagttct tccagcaact catttttttt ctgctttgtt accacgtttc ctcctttcca
     3001 acaaaaatgg ccccgtttcc aaggaagcgg ggccatttta ataaggattg gctttttact
     3061 cgcttgcctc ggcgggtgtt ttcactttaa gccctttttt taagggcgcc tgctttctct
     3121 ggcttgcttg ttaaattgtg aaggtgtaga tcaggctttt attgctgctg ctgttgcttt
     3181 tgaaacatca agcttgacgc ccgggctcat cgtcaacgaa agagtcattg tgaaaatgta
     3241 ggttcccttg agtgatgccg gttttgccct gactatggca gagaggaagg ccagaatgtt
     3301 cttctggatg tcttcgtttg agaaagatat tttgccaact ggcacatgca tgtctgcaaa
     3361 cttggatatt ctgtactcga ttcttccagc cttgaactct tttactgctt tatcaacttc
     3421 catcgtgaca gtgccggcct tggggtttgg catgagaccc tttgttccga ggatcttacc
     3481 aagcttacca acgctgccca tcatgtctgg cgttgcaatg gcaaggtcaa aatcaaacca
     3541 gccacccttg atcttttcaa ttatatcgtc atcgccaaca taatcggcac cagcttcttt
     3601 tgcctcagtt gctttttcac cctttgcaaa aacgagcacc cttactgttt ttccggtacc
     3661 tgccgggagt gaaacagtgc ctctgatgta ctgatcagcc tgctttggat caacaccgag
     3721 cttgagaccc acttcaattg tctcatcaaa ctttgtgctt ttgagcttct taaggatatc
     3781 tatagcctct gaaacatcat aaagctttgt tttgtcaaag accttttcca gttttttgta
     3841 tctcttgcct ctaaacattt tgcctcctgt ggtacgagcg ggccttcggc cctcccacga
     3901 attttaaaaa ctacttgcct atatcaacgc ccatcgaacg ggcagttccc attaccattc
     3961 tctttgccgc ttcaatatca aaagcgttta cgtccggcat tttgactttt gctatctctt
     4021 cgacctgcga cattgtaagg accccaactt ttttcttgtt tggctcacca gaacctgctg
     4081 gaaccttgag tgccttgcgg ataaggtcag aaactggtgg ggttttgaga ataaaatcaa
     4141 aagagcggtc ctcataaacc gatataatga ctggaataat catgcctggt ttgtctttgg
     4201 tcttctcatt gaagtccttg cagaactgca tgatgtttac accgcgctgt ccaagtgctg
     4261 gaccaactgg tggtgccggg tttgccttgc cggcttcgat ctggagcttt acgtatcctg
     4321 taattttttt cttaactgcc atgaaagact ctccttaaat aacctctatg ttgacaaagt
     4381 caagttcaac aggcatttct ctaccaaaca ttgaaagcct gactttggct ttttcctttt
     4441 cctcattgat tgcttcaaca atacctatca tctcattgaa tgggccacca acaactctta
     4501 tggtatcacc gacactgaag ttgagtgccg ctttggcttc ttcaagacca acccttttga
     4561 gcatttgctg gacttcttct tcttcgagtg ggagtggttt tccacctgaa cccacaaacc
     4621 ctatcactcc aggtgtgttt cttacaacat accatgactg gtcagtcatt atcatttcaa
     4681 ccagaacata ccctgggtaa accttttccg gaagtacctg gcgtttgcca tcttttagtt
     4741 tgacctggta gtccactggt acttcaatac ggaaaataag gtcctccata ttcatggact
     4801 gaacacgttg aataaggttt atcttgacct tgttttcgta tccactatag gtgtggagta
     4861 tataccagtg tggttcggga agagagctca ttgtttattt atttccgccc tcgtttgatg
     4921 gtggagtggt ctgaccgcca ttgcctgggg ttggagtgcc agtattttcc tgattttccg
     4981 gaacatagcc tttaactcca gcttttttct ggctttccat ccattcatca agcttttgct
     5041 gggattcaaa cttctttact tctccattct ggagcgcgac aggatcgtag tagtcctgtg
     5101 caagccagct ctggaaagac gagaataccg agtcaaaagc ataaaggatc actgcccaga
     5161 tcatgataaa gactatgaca attgttgttg ccttcacaag ccttttccaa gtgggccatg
     5221 tgaccttgtt ggtcatctca taccacatat ctttgaagaa gttggtgcga agcttattca
     5281 tatttacctt tctggcaggc tcggagggac tcgaaccccc aacactcggt tttggagacc
     5341 gatgctctac caattgagct acgaacctgc ataaagcata aagccttaca aaacagcaat
     5401 tttaagtctt tgatgcagct tgtcaagata ttttgttaac gtttgcagga ggcgagcaca
     5461 gggaatggga agggcttgca tttgaatcgt ttatatgtta tctaataaac aattatgcag
     5521 agggcaacac tgttcttcaa caaaatccaa cttgaggatg ccgacaaatt aataaaaaaa
     5581 caggcaaaaa caaggctggt taccctcttg actgtgacta cactcatatt atcaggtctt
     5641 acagctgctt tttttgtgcg atttccagca caaaacactc aacctgtcgc acaagatgta
     5701 tcaaaacagg acaattttga gaaaaagatg ttttcgattg gcgaacagat cgtaaaatca
     5761 acaactcctt gcaaagagcc ccaaaaggca gagccaatga caatttcacc tcaaaaaata
     5821 gctgccaagc ctctggaatc tgcacccaaa acccagccaa aatcagttcc tactaagatc
     5881 gctcagtcga caaaaacacc ggcaagaaca atcacaaaaa cacctacgac tacgaaacca
     5941 aaaacaacca cccaagcagc tgcaaaaacc acccaaaaaa ctgcccaggg tggagacaac
     6001 cttcctgtga aaacgagaga tttaggcaaa atcgatgaaa gtctccctgc ggccaagctt
     6061 gatccgatgc cggtaaacgc aaactttgga atccccgaat actggccact gcccgtaaaa
     6121 gtgtcagatc cttacggtta tttgccctgg aggggaaggg tccatgcagg aatcgacctt
     6181 accgccccat gcgactccct tattctggcg gctggcaatg gaaaggtcat cagggcagaa
     6241 tggtatgctg gatatggcaa atgtatagat atccagcacg cctccggata cataaccaga
     6301 tacgcccacc tcaccgatta cttggtaaag gtcggggact tcgtagaagc cggggaatgg
     6361 attggcaaag tgggttcaac aggaaactca agctgctgcc acctccattt tgaagtccac
     6421 ctcaacgggc gcaccataga tccagaaagc atacactggc tcaatagaac ttactaatca
     6481 ataatagagg tttcaaatgc aaatagactt ctctgacaaa gaatttttcc gccagcttgg
     6541 ggctacactc aacaatgcaa cctgggaatt gattgacaaa gatgagcgct ccagcgaaga
     6601 aagcatgaaa atgatagaaa tggcacatgc ttcatgctat ttttggaccg agtttggagg
     6661 cccgctcaac gttcagaggg gccactggct tgtctcaagg gcatatgctt gcgtaaaact
     6721 tccctggcct tgtttgcacc atgcaaaaat atgcatggag attacagaaa aacaggggct
     6781 gaaagatttt gacctggcct ttgcttttga ggcaatggca agggcgtatt ccatttccgg
     6841 caacgaagca gaaaaagcaa attatctgga aaaagcaaaa agggttgggg aacaaataaa
     6901 agactctgaa gataaaaaca tcttctttca ggaactcgac agaggcccgt gggtctgaac
     6961 accaaacaga cagttttgta ttgaatttca taaaactctc aaaacggcat tttaaaaact
     7021 tgagccaaga tatcaccgat tgaaaaaact ctgtagaatt taaatccatg gtatctgctg
     7081 gccattcatt tcggcaatga gtactatctg gccaaggtgg tatgcttcat gctcagcaac
     7141 atggtacatt atccacgcaa catgtgtcga ggctttcttt ccgtatggaa attccaatgg
     7201 tttatctttg ttctcctcga gccacttcac agtttttgct ctagtcctct caagaagggc
     7261 aatgatctct tctataccca ttgtagaaat atcgtaattt tcagcttctt tttctttcat
     7321 gaccaatcgg tgtatccacc caacctcagc ctgaacgata tgtttcaaaa ttcttcttat
     7381 ttttccagga ctgtccttcc ttggtttttt gcttggcaat ttataatcca gcgtttctac
     7441 aaaaagcgac tgcaaaatct ttatcgttct tgatctcgtc cagtgaaaaa actttattgc
     7501 atcctgacca gtcatctaaa tctccttttg aaaagctcat tttatcatgc acaaacacaa
     7561 aaagc
//
LOCUS       DAOSRI010000047         7537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9151_length_7537_cov_3.699679, whole genome shotgun sequence.
ACCESSION   DAOSRI010000047 DAOSRI010000000
VERSION     DAOSRI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_7537_cov_3.699679"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2801)
                     /locus_tag="PK717_02355"
     CDS             complement(<1..2801)
                     /locus_tag="PK717_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46537.1"
                     /translation="MKKLTSILLVIALFMGFPPRKIIAEEEEIVLFDNFTTSSPNFKN
                     GPSKPTVFSTSQSYKIIGIGTVHFNNGKGSKNYGSISLKHEDGTVYGPWQSYAPPDVK
                     GSEKIFWIVEPNVVIKPGKYEVVDSDKDTWSWTDESGGCGLALVAANKVKEQPPSGGP
                     GGKAKTIVLTIGKPTMIVNGKSVEIEKGKNTGPIIIKGRTMLPIRSLIETLGGTIDWN
                     SKEQKITLKLEEISVELWIGKNTARVSGREITIDQPPVIVGGRTMVPLRFVSENLGCS
                     VVWDAKAKTITIKFFGKLIPANGGSVGSDDGLASVAFPPHFASANTSASITMVSPPKD
                     NFSGQYKVEVSAEKSGTFSVAIKASFKFNQKKDRVLAESLDEQTGQWVKCGDSLDYDP
                     DTGVLYFNDNFGQSSKTITTFSTSGTMQAAPPQNARVYRAEHQQNWLDSGVNTVLPDS
                     NFRITYFRAGRPCLGCLEDRVPDDSAWEIRGSGGQNNDPTTDNYIEDLDKALNEAYER
                     LTEFDGDSLFQPLQTPFDVIVMDCGRGVQGETAIGGGTRRRMYISCNDLDDYENMKLV
                     ATHELIHVFQGQRYTNGRAGTNRWFVEAVATGLSAYITNVSEGAKRDYYTGGRSGFMD
                     YENYLWTTFYSNNARSYYTCGLFVEWMLNVYGDSAITNIYSRAEELDLDRLSGFFSGA
                     GTNLEDAFFTYCHNLMVNPLGGWGMNATIKANIVRGGTIKPNNKFSPQTKLVTFEGDV
                     DVMSAQRVFLSTSAQITQTGLLVIDSSETKDGLRTISYDLVSSRSDDYANSGGPIDRY
                     ESTLPYRKTLSVMYFGSSSSSTRKGFDQMIINQGNRRRMHAKIQYYLLLEPIKTKFER
                     GIVEWTTNELGNIPESYIKGYNIFLNGIKVSGKDPIKPVKGTISFKHSEILRNLDDRV
                     QIQVVDKYGNTWPYIQESK"
     gene            complement(2945..7504)
                     /locus_tag="PK717_02360"
     CDS             complement(2945..7504)
                     /locus_tag="PK717_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46538.1"
                     /translation="MTKDGKFISEFGSFGTGERQFANPVGVDYKNDNIYVADAGNNCI
                     KQFTVDGTFKKIIGSAGNGNGKLNSPSDVVVVSSVNKMFVADSGNNLVQVFSLSGEFI
                     NQFGGYGQAPGQFSNPTGISSDGRKLIVADSGNCRIQEFTLDGKPVKQYGFKGRGKEQ
                     LFFPQDCSYDQSGKIYVSDTYNDRVVVFDNASGRSWIYGRSGGPGRAIFFSKSGEENE
                     DTNDPDIKKAAGFFSFPSGLACIEDKVIVSDTQNLRLQSVPYSAIFDFPRVDTESFVP
                     QTSNVVWFTVAPKILDFGVMPVGEQGQRRIEVRNWTGGALTGKIEISQEIPFLSVDPP
                     TFVGDVNFITVKIDTTGMTAGQAMKAVIKIATNKGDQSIEAVVLPSDGFGYSLPTEQG
                     YFVEMECNNPASLEITINPQNGFDRNVSFSYAMPLKKCLTHPSGKAIENLSCDPKNFE
                     LSNVTVEFKPSSVKIRENNKTTMTLIPKGNVTPGIYEIAVTMNGAGAANKEVKFTFIL
                     LVNPCTNPATDKQVPRALLTETFTAVWCQYCPFHREGQYRMAEEYGYAEIIPLAYYCD
                     APNDNSGMTQESHHYRYRWYTQEGLPTTMFNGVWNFSEGDKPFERQAPPPDRLPDRKM
                     SGTSFSYLRFKQRTETYRHMTSPLSLFLQATVDGDEGDAYLEINTLSDVSAYKDLNVY
                     FVLVENNVLFEATNGEREHSLTVMKFLYEKGQKKSPKDEALGDPISLAPGTITKRRIH
                     FEMPGKNYDWKTLTKNCYVVAWIQDNTKKEILQSTMVDLAQPLLTSYTFKQVNGQQTS
                     VQAGSRANFKYILTNTGNIFMDFGIQMTHLGGQNWENSLLVDKVPSPNGGRITLDPMQ
                     SSLIELSVEVPPGTSEGTESLFRVEVSDDLSGQSSSSDLKLMVEPAKPPSFEISSSTK
                     FVEVEPSTETSFNLTINPINEYNNPVTLQLAQESQKYFQAKFDPPNGVPPFETKVTIK
                     SSEQLEYFEDGYKIKILATGSDARGEKIEKDITIRATAKLLSLTLKPERKVITSCAIN
                     EICRQTEVKINLEPSDIQLKEARLDFVYDGRYLEVTNVSLGQMMTRFGEIADMQYDIK
                     PDRVNIRIKREKGVVADQNENTIVAITLKSTGDKIEIDNVRIGLENVTLLSASGSKAL
                     TKSVDETLSIRKNARPPVIHLITPIQKDSPWPDEAESKRRSEQGQINYRDITTTEKSL
                     KISGKCESEEGVKQLTLFVGGNKVQLASDGTFDYTMNLKEGFNNIVIVCQNFTGESDG
                     IMVIAVLDTTPPDLIIDRPSEDQFSMDYSMTTGQNFVEFRGFTEEKAKVTVAGRQVQV
                     RKDPITDDETKRFFFTEKVQLAQGLNEIEVSACDENGNCRQYTYKVTYDPKAGNGKTT
                     KIELWLGKASMLVNGETVKLVTAPTASSPPLPKDLAGSTFMPIAEVAKALDVEVGWNG
                     KDKKVTLTQKLDNGKKKVIELWIGKKTAKIDGKEVFIDSAKKLYPTIVSGKTLLPLRF
                     VGDALGALVEYESKAKKITLTYPKP"
BASE COUNT         1698 a         1661 c         1725 g         2453 t
ORIGIN      
        1 tgtttgcttt cctggatgta tggccaggtg ttgccgtatt tgtcaacaac ctgtatctgc
       61 accctatcat caaggtttct caatatttca gagtgcttaa aagaaatggt ccctttgact
      121 ggcttgatcg ggtcttttcc ggacactttt attccattca gaaaaatatt gtagcctttt
      181 atgtaacttt cggggatatt tccaagctcg tttgttgtcc actcgactat tcccctctca
      241 aactttgtct ttattggttc caggagcagg taatactgga tttttgcatg catcctcctt
      301 ctatttccct ggtttatgat catctggtca aacccttttc ttgtggaact ggaagagctg
      361 ccaaaataca ttacgctcaa ggtttttctg tatggaaggg tgctttcgta cctgtctatt
      421 ggtccacctg aattggcgta atcatcagat ctgctactca ccagatcata agagatggtc
      481 ctcagaccgt cttttgtttc gcttgaatcg atgactagca agccagtttg ggttatttgt
      541 gcagatgtgg agagaaaaac cctttgtgct gacatgacat ctacgtcacc ctcaaaagtt
      601 accagttttg tctgtgggga aaacttgttg tttggcttta ttgttcctcc ccttactatg
      661 tttgctttta tggtggcatt cattccccat ccacccagcg gattgaccat caggttgtga
      721 cagtaagtga agaaggcatc ttcaaggttt gtgccagccc cggaaaagaa ccctgaaagt
      781 ctgtcaaggt caagctcttc tgctctggaa taaatgtttg tgattgctga atctccatac
      841 acattcagca tccattctac aaaaaggcca catgtatagt aacttcttgc attatttgaa
      901 tagaatgttg tccagagata gttttcatag tccatgaagc cgcttctccc gccagtataa
      961 taatctcttt ttgctccttc tgaaacgttt gttatgtaag cagagagccc cgtggcaacg
     1021 gcttccacaa accatctgtt tgtgcctgcc cttccgtttg tgtaacgctg gccctgaaaa
     1081 acatgtataa gctcatgtgt tgcaaccagc ttcatgtttt cgtaatcatc cagatcgttg
     1141 cagcttatat acattcttct cctggtcccg ccgccgattg cggtctcacc ctgcacccct
     1201 cttccacagt ccattactat cacgtcaaat ggtgtttgaa gcggctggaa aagactgtca
     1261 ccatcaaatt ctgtcagcct ttcgtatgcc tcgttcaatg ctttgtccag gtcctcgata
     1321 tagttgtctg ttgtcggatc attgttttgc cctccgctgc ctctgatttc ccaggcagaa
     1381 tcatcgggaa ccctgtcctc gaggcaacca aggcatggcc tcccggccct gaagtatgtg
     1441 atccggaaat ttgagtcagg taataccgtg ttcacaccgc tgtcgagcca gttttgctgg
     1501 tgctctgccc tatacaccct cgcgttttgt ggtggggcag cctgcatggt gcctgatgtg
     1561 gagaaagtcg ttattgtttt tgaggattgc ccaaaattgt cgttgaagta aagaacaccg
     1621 gtatctgggt cataatcaag cgaatcaccg cacttgaccc attgccctgt ctgctcgtca
     1681 agggattctg ccaggacacg gtcctttttc tggttaaact tgaaacttgc cttgatcgca
     1741 acactgaaag ttccggattt ttcagctgaa acctcaacct tgtactgccc tgagaaattg
     1801 tcttttggtg gtgaaaccat tgttatcgag gcgcttgtat ttgctgatgc aaaatgaggt
     1861 ggaaaagcaa ctgatgccaa tccatcatca gaacccaccg agccaccatt tgcaggaatg
     1921 agttttccaa aaaacttgat ggtgattgtt tttgctttgg cgtcccagac gaccgagcat
     1981 ccgagatttt ctgagacaaa tctgagcgga accattgtcc tgccacctac tattacaggt
     2041 ggctgatcaa ttgttatttc tcggccggaa actctggcgg tattcttgcc aatccagagt
     2101 tcgactgata tttcctcgag cttcagggtt attttttgct ctttgctgtt ccagtcgatt
     2161 gtcccaccca gtgtctcgat tagagacctg atcgggagca ttgtcctgcc ttttatgatt
     2221 attgggccag tgtttttccc cttttcaatt tccaccgatt tcccgtttac aatcatggtt
     2281 ggtttgccaa tcgtcagcac tattgtcttg gctttgcccc caggacctcc ggaagggggt
     2341 tgttccttga ctttgtttgc agcaacgagc gcaagtccac acccaccaga ctcatcggtc
     2401 catgaccacg tatctttatc agaatcaaca acctcatatt ttccgggttt aatgacaaca
     2461 tttggttcga ctatccagaa gatcttttct gaacctttca cgtctggcgg ggcgtatgat
     2521 tgccaagggc cgtatacggt tccgtcttca tgtttgagtg atatcgatcc ataatttttg
     2581 gagcctttgc cgttattgaa atgtaccgtg ccaatgccaa tgatcttgta tgattgggac
     2641 gtgctaaata ctgttggttt tgagggacca tttttgaagt ttggagatga agttgtgaaa
     2701 ttgtcaaaaa ggactatttc ttcttcctcg gcgataattt ttcttggagg aaaacccata
     2761 aaaagggcga ttaccaataa aattgatgtt agttttttca attaaaacct cctttttaaa
     2821 gataacaaaa ttgagtttag tttggtgact gaataaagaa agaggccagt ttttagaaat
     2881 ttgagaaata aaaaagccgc cctaaaaggg cggctttttt attgtgtcgg tgattgataa
     2941 ttttttatgg ttttgggtaa gttagtgtta tcttcttggc tttggattcg tactcgacaa
     3001 gtgcaccgag ggcatcgcca acaaacctca atgggaggag tgttttgccg ctgacgattg
     3061 tcggatagag cttctttgct gaatcgatga aaacttcctt gccatcaatt tttgcagttt
     3121 tcttgccgat ccagagctca ataacctttt tcttgccatt gtcaagtttt tgggtgagtg
     3181 tgactttttt gtccttgccg ttccatccga cttcgacatc gagtgctttt gcaacttcgg
     3241 ctattggcat gaaagttgat cctgcgaggt cttttggaag tggtggagag gacgctgttg
     3301 gcgcagttac aagttttaca gtttctccat taacaagcat tgatgctttg ccaagccaga
     3361 gttctatctt ggtggttttt ccgttcccag cttttggatc ataggttact ttgtatgtgt
     3421 actgcctgca attaccgttc tcatcgcatg ccgagacttc tatctcgttg agcccctgtg
     3481 caagctggac cttctctgtg aagaagaacc tctttgtttc atcatccgtt attggatctt
     3541 tcctgacttg gacctgacga cctgcaacag tgacttttgc cttttcttca gtgaaccccc
     3601 tgaactcaac aaagttttgg ccggttgtca tggagtagtc catagaaaac tggtcctcac
     3661 ttggcctgtc gatgataagg tctggtgggg ttgtgtctag aacagcaatt accattattc
     3721 catcgctctc accggtgaag ttctgacaga ctatgacgat gttgttgaac ccttccttga
     3781 gattcattgt gtaatcaaat gttccgtcgc ttgccagctg gactttgttt ccaccaacga
     3841 aaagggtgag ttgttttaca ccttcttcag attcgcactt acctgagatt ttgagcgatt
     3901 tctcggttgt ggttatatcg cgatagttga tttgtccctg ttctgacctt ctcttggatt
     3961 cggcttcgtc tggccacggg gagtccttct ggattggggt aatgaggtga ataacaggtg
     4021 gtcttgcgtt tttgcgaata gagagtgttt catccaccga ttttgtaagt gccttgcttc
     4081 cggacgcgga aagcagagtg acattttcta gtcctattct gacgttgtcg atctctattt
     4141 tgtcaccggt tgatttaagg gttattgcga caatagtgtt ttcgttctga tctgccacaa
     4201 cgcctttttc cctcttgatc ctgatgttga ccctgtcagg tttgatgtca tattgcatgt
     4261 ctgcaatctc gccgaaacgg gtcatcatct gtccgagaga aacgtttgtc acttcgaggt
     4321 acctgccatc ataaacaaaa tcaagcctgg cttctttgag ctggatatcg gatggctcaa
     4381 gattgatctt tacctcagtc tggcggcaga tttcattgat tgcgcaggac gttataactt
     4441 tgcgctctgg tttgagtgtc aggctgagca gttttgcggt tgccctaatg gtaatgtctt
     4501 tttcaatctt ctcacccctg gcgtcagaac ctgtggcgag gattttgatt ttatagccat
     4561 cttcgaagta ttcgagctgc tcggaagact tgattgttac ttttgtttca aatggcggaa
     4621 cgccatttgg cggatcaaat tttgcctgga agtatttctg ggattcctgt gcaagctgga
     4681 gtgtgactgg attgttgtac tcgttgattg ggtttattgt gagattgaat gatgtttctg
     4741 tggatggttc cacttcaacg aattttgtgc ttgaagaaat ttcaaagctt ggtggtttgg
     4801 ctggctctac cattagtttg agatctgaag aagaagattg tccagaaaga tcgtcagaga
     4861 cttcaacacg gaagagactt tcggtgcctt cagaggttcc tggcgggact tctactgaga
     4921 gttctatgag tgatgactgc attgggtcaa gtgttatcct gccaccattt ggtgatggaa
     4981 ctttgtcaac cagcagcgag ttctcccagt tctggccgcc aagatgtgtc atctggatgc
     5041 cgaaatccat gaatatgttt ccggtatttg taaggatata cttgaagttt gccctagaac
     5101 ctgcctggac tgaggtttgc tgaccgttga cctgcttgaa tgtgtaagag gtgaggagtg
     5161 gctgagcgag atcgaccatt gtgctctgga ggatttcttt ctttgtgttg tcttgtatcc
     5221 aggcgactac gtaacaattt tttgtgaggg tcttccagtc ataattcttg cctggcattt
     5281 caaagtgtat gcgacgtttt gttattgttc ctggtgcaag gctaattgga tctccgagtg
     5341 cctcatcctt tggtgatttt ttttgccctt tctcatagag gaacttcatt actgtgaggc
     5401 tgtgctctct ctcgccattt gttgcctcga aaagaacgtt gttttccaca agcacaaaat
     5461 acacattcaa atctttgtat gctgaaacat cgctcaaggt gttgatttcg aggtaggcgt
     5521 caccctcatc accatcgact gttgcctgaa ggaacaacga aagtggcgaa gtcatgtgtc
     5581 tgtaggtttc ggttctctgt ttgaaacgaa gatagctgaa tgatgttcct gacattttcc
     5641 tgtctggaag cctgtctggt ggtggggcct ggcgttcaaa tggtttgtca ccttcggaga
     5701 agttccatac accgttaaac attgtggttg ggaggccttc ctgtgtgtac cagcgataac
     5761 gataatgatg tgattcctgg gtcattccag aattgtcatt tggtgcgtcg caatagtatg
     5821 caagaggaat tatttctgca taaccatact cttcagccat tctatattgg ccttccctgt
     5881 ggaatggaca gtactggcac cagacagccg taaaggtttc tgtgagcagg gcccttggta
     5941 cctgcttgtc ggttgccgga tttgtacacg ggtttacaag aaggataaaa gtgaacttga
     6001 cctccttatt tgctgcacct gcgccattca ttgtcactgc aatttcatag ataccagggg
     6061 tgacattgcc ctttggaatc agtgtcattg ttgttttgtt gttctccctt atcttgacgg
     6121 atgagggttt aaattcaaca gtgacattag ataattcgaa attttttgga tcacaactca
     6181 aattttcgat agcttttccg gatggatggg tgaggcactt tttgagtggc attgcgtagc
     6241 tgaagctcac gttacgatca aaaccatttt gtgggtttat ggttatctcg agactcgccg
     6301 gattgttgca ttccatctcg acaaagtaac cctgttcagt gggaagcgag tagccaaaac
     6361 catcagatgg gaggactact gcctcaatgc tctgatcgcc tttgtttgta gctattttaa
     6421 tgactgcttt catggcttga ccagcagtca tgcctgttgt gtctattttt acagtgatga
     6481 agttcacatc accaacaaat gttggtgggt ctactgatag aaatgggatt tcctgactga
     6541 tttcgatctt gccggtgagt gcaccaccag tccagttacg gacttcaatt cttctctgcc
     6601 cttgctctcc aactggcatt acaccgaaat cgagtatttt tggtgcaact gtaaaccaca
     6661 caacgttgct tgtctgtgga acgaatgatt ctgtgtctac tcttgggaaa tcgaaaattg
     6721 cgctatatgg cacagactgg agcctgaggt tttgtgtgtc ggagacgatt accttgtcct
     6781 cgatgcatgc aagaccgctt gggaaagaga agaaaccagc agcttttttg atatctggat
     6841 catttgtatc ttcgttttct tcacctgatt ttgagaagaa gatggctctc cctggcccgc
     6901 ctgatcttcc atatatccat gatcttccag aggcattgtc aaaaacaact actctgtcgt
     6961 tgtaggtatc tgaaacgtag atctttcctg actgatcata agagcagtcc tgcgggaaga
     7021 aaagctgttc ttttcctcta cccttgaaac cgtactgttt gactggtttt ccatcgagtg
     7081 tgaattcctg gattctgcag ttgccggaat ctgcgactat gagttttctg ccgtctgatg
     7141 aaattccggt tgggttgctg aactggcctg gggcttggcc atacccacca aattggttga
     7201 taaactcgcc ggaaagcgag aacacctgaa caaggttgtt tccagagtcg gcgacaaaca
     7261 tcttgttgac tgatgaaacg acgacaacgt cagagggtga attcaacttt ccattgccgt
     7321 ttcctgctga gccgattatc tttttgaaag ttccatctac agtgaattgt ttgatgcaat
     7381 tatttccggc atctgccacg taaatgttgt cattcttgta atccacgcct actggattgg
     7441 caaactgcct ctcacctgtg ccaaatgaac caaattctga tatgaatttg ccatctttgg
     7501 tcatgattat gacttttttt cttgcaaaat ccgcaac
//
LOCUS       DAOSRI010000048         7491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9208_length_7491_cov_4.059978, whole genome shotgun sequence.
ACCESSION   DAOSRI010000048 DAOSRI010000000
VERSION     DAOSRI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_7491_cov_4.059978"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK717_02365"
     CDS             <1..553
                     /locus_tag="PK717_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46539.1"
                     /translation="EYPDISGYYIVWCDHRNDYHDYGKPRSKKDGTNSGDIYAHRIST
                     GETFPVENTNDSSIYPQISGSIATWHMSKKGSVGELATIMVADLSIPKITPIPASSQI
                     SHKYPPLIDGDLVVWMDMRQYKLGKTNIYGYRISSRTEIQLTNTNGRDKIEWLSSPYL
                     VYNHNTDIKNQDGRGDLHIIKIR"
     gene            complement(550..1305)
                     /locus_tag="PK717_02370"
     CDS             complement(550..1305)
                     /locus_tag="PK717_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR46540.1"
                     /translation="MKNLLVSDMTHFEERLPAIKQAGTGIEIQSFFLSLALDNSQAML
                     ESHKAALSDFSDEKCLHGPFVGLMPGCMDAQIKQVTAKRMAQALFVAQELGCSRMVAH
                     FGMNCKAQRRWDYVDRSVEFWTEFSRICPEGFSIHVENVYDDRPDDILSLSKKLENPK
                     IGITLDIGHANIFSKIPVWKWIEAYGSRLTHVHLHDNNGEEDEHLPLGQGSIDLDRVI
                     VALEEHAPMAVWSIEAEPATSLVWLKSRGIWAG"
     gene            complement(1306..2160)
                     /locus_tag="PK717_02375"
     CDS             complement(1306..2160)
                     /locus_tag="PK717_02375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR46541.1"
                     /translation="MRLRKISFVRLPNDYGWDIISCMDDKEVGKYWDENARAWTDLSR
                     LGYDICRDQFNTPGFVAMLPEIGGLEGLDIGCGEGNNTRIFASLGASMTALDISPTFI
                     EAAKAMEQEKPLGIKYVLGSALDLPFEDSSFDFCVATMSFMDIPGPEKVLAEAFRVLK
                     PGGFLQFSITHPCFDAPVRFWVFDEDGNKKALAVGGYFDELGGDILEWIFAVTPQELK
                     DKYPKFKTPCFRKTLGSWINLLIDTGFMIEKLGEPRPTEEVCEKYPSLADARLVSLFL
                     HIRARKPR"
     gene            2262..2978
                     /locus_tag="PK717_02380"
     CDS             2262..2978
                     /locus_tag="PK717_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOR46542.1"
                     /translation="MKEKTCTVCSASENHMMPMSMLRKPIIDLIKKDHPDINENGYIC
                     TEDLNKYRAKYISSLLEEEKGELSSLEKQVVKSIEDQENITKNLGEQISRKVTFGEKI
                     ADKVADFGGSWTFVIIFFASILTWMTINIIALRNPFDPYPFILLNLVLSCVAAIQAPV
                     IMMSQNRQEARDRMRDENEYAINLKAEIEIRHLQDRLDHFIMKEWQRLLEIQIVQTDL
                     IEELAEKDVLGGNFGKPTRK"
     gene            3209..3859
                     /locus_tag="PK717_02385"
     CDS             3209..3859
                     /locus_tag="PK717_02385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR46543.1"
                     /translation="MARKKDQKRYYYDHEPYYAKICCEGGTGWPIESDVSQRFLLKWA
                     REQIFPEYATILEVGCGGGELAIELAKRGVKVEACDLSETAIRICQSKNPPENLAFFA
                     ANAMTHKGYPNPPYDWIIANQVLQSIIGGDRVFLLKRMRENLTTTGTIVVATMLGVPE
                     KFKEEFNPITFVNRTNTRFFADLRMFAKEIAEARLRVTRHIKIDDHYRIFFLERLP"
     gene            3899..4381
                     /locus_tag="PK717_02390"
     CDS             3899..4381
                     /locus_tag="PK717_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46544.1"
                     /translation="MDIERGAKPGDIPELIKLHRYGEELLEGNRSQFLMDPMIITDEQ
                     LEDDFNEWLENPEIGFFVAKENGKLAGFVLAMIIEEPDDLINVPYVSINELAVSKEFQ
                     GSGVGTTLMKRVEEWALENDINIVQLNVWEFNRKAKTLYEKLGFKTIQCQMEKILSKR
                     "
     gene            complement(4472..6406)
                     /gene="gatE"
                     /locus_tag="PK717_02395"
     CDS             complement(4472..6406)
                     /gene="gatE"
                     /locus_tag="PK717_02395"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOR46545.1"
                     /translation="MNYDDLDYYQSLGFKSGLEIHQQLNTQKKLFCHCPVGYQKVEPD
                     ARIVRHMRPTLSEMGTYDGTALMEFKTHKEVVYLLYRDYVCTYEMDDTPPFPINQQAL
                     DYAIEIALMLNCQLVDEVHITRKQYLDGSIPTGFQRTAIIGVDGWVPYKGRKIRIQQL
                     ALEEDSCREVSDIGHRIVFKTDRLSTPLIEIVTYPDMRTPRECAEVDELLGRLLRASG
                     LVRRGIGTVRQDVNSSINGAPRIEIKGVPKTWYIPKMLDTEVKRQRKLLEIKEKLVSY
                     GLVPNQKLDSSLREQERQIELEKIRNSNVADVTDMLLKSSSPQVKNAISRGDKIWAVK
                     LPQFHEVLSAELQPGWQFADEFGARVRVIACIDKMPNLTFRSGKLLYPGDWEPIAQKL
                     NAYPTDEIVLVWGPEADLKTAVNEVILRADEAVRGIPEETRQHMQDGYRTDFERVLPG
                     PDRMYPDTDSPPVRLTRERVDRIRADMAQRPWEREEKYAKLGLSKCVSDKLATHQRAK
                     LFDKLVDLGILPKLAAELIVDWLTDLKRQRLIVSRLTDDTVIKVMMEAKEKGLPKEAI
                     KNELKAFASDSNYIAKTQSMATDSVIKFIVEGVVDRSMQLKFSSKEAQTRWAKGQAMQ
                     SLYGRAHGSKVAKVIEEALS"
     gene            complement(6492..6704)
                     /locus_tag="PK717_02400"
     CDS             complement(6492..6704)
                     /locus_tag="PK717_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46546.1"
                     /translation="MSCYLRHLDHLFVETDLDPLNKQDRKKLDMAIRLSIGLIDSSCS
                     EVWAKIKEIGPENKDLFLKVKQELNK"
     gene            complement(6750..>7491)
                     /locus_tag="PK717_02405"
     CDS             complement(6750..>7491)
                     /locus_tag="PK717_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR46547.1"
                     /translation="RANAEKFGIDPDRIGIHGGSAGGHLVALMGLANDGDGLEGPGLE
                     GISSRVSCVVDYFGPSDLTFMTKEINDKKLPGVELIKDFLGCYPSDCPDKATQASPVT
                     YADKNDPPILIQHGNKDLTVPYSQSVVLAKKLYENNVTCALIKVKNGGHGFSGKNIRP
                     SRDEITLKTVSHIARFLESAVFADLDLNGDIDYADAIEMATCFGAEGTNAEGKEAPDF
                     WNPLADLVPDGKIDMADWDAFWDAWLGK"
BASE COUNT         2091 a         1782 c         1634 g         1984 t
ORIGIN      
        1 ggaataccct gacatctccg gttattatat tgtatggtgc gaccatagaa atgattatca
       61 cgattatggc aaaccacgat ccaaaaaaga tggcaccaat tctggagaca tatacgcaca
      121 cagaatttca acaggtgaaa cattccctgt tgaaaacaca aatgattctt cgatataccc
      181 tcaaatttca ggcagcatcg caacatggca catgtctaaa aagggttctg tgggtgagtt
      241 ggccacaatc atggtggcag acctgtctat accaaaaatc accccaatac cagccagcag
      301 ccagatttcg cacaagtacc ctcctctgat tgatggagac ctggttgtct ggatggacat
      361 gagacagtac aagcttggca aaacgaatat ctatggttat agaatcagtt ccagaactga
      421 aattcaactc acaaatacaa acggcaggga taaaatcgag tggctatctt ccccatacct
      481 ggtctacaac cacaataccg acatcaaaaa ccaggatgga agaggagatt tgcacatcat
      541 caaaattcgc tagccagccc agatccccct tgacttcagc catacaagcg aagttgcggg
      601 ttcagcctct attgaccaaa cagccatcgg agcatgctcc tcaagcgcaa caatgaccct
      661 gtccaggtcg atgctcccct ggccaagtgg aaggtgctcg tcttcctcac cattattgtc
      721 gtggagatgg acgtgggtca gcctgcttcc atacgcttca atccacttcc agactggtat
      781 ttttgaaaag atgtttgcat gtccaatatc tagcgttatc cctatttttg ggttttcaag
      841 ttttttggag agcgacagga tgtcatccgg cctgtcatca tagacgtttt caacgtggat
      901 cgagaaccct tctgggcaga ttctggaaaa ttctgtccag aactcgaccg acctgtccac
      961 ataatcccat cttctctggg ccttgcagtt catcccgaag tgggcaacca tccttgaaca
     1021 gccaagttcc tgggcaacaa aaagcgcttg cgccatcctt tttgccgtta cctgttttat
     1081 ctgggcatcc atgcagccgg gcatgagacc aacaaatggc ccgtgcaggc acttttcatc
     1141 agagaaatcc gagagtgcgg ccttgtgtga ttccagcatt gcctgcgaat tgtccagcgc
     1201 aagcgagagg aaaaaggact gtatttcaat cccagtgccg gcctgtttta ttgctggcag
     1261 cctctcctca aaatgcgtca tgtcggacac gagaaggttc ttcatttatc tgggctttct
     1321 tgctctgatg tgaaggaaga ggctcacaag cctcgcgtca gcaagagaag gatatttttc
     1381 gcaaacttcc tctgttggcc ttggctctcc aagtttttct atcataaaac cagtatcaat
     1441 caaaagattt atccatgagc caagcgtttt tctgaagcac ggggtcttga attttggata
     1501 tttatctttc agttcctgtg gcgtgacggc gaatatccat tccagaatat cgccacccag
     1561 ttcatcaaaa tacccgccaa cggcaagtgc ctttttgtta ccatcttcat caaacaccca
     1621 aaacctgaca ggtgcgtcaa aacatggatg tgttattgaa aactgaagga atcctcctgg
     1681 ctttaagacc ctgaatgcct cagcaaggac cttttctggt cctggaatgt ccataaagct
     1741 cattgttgca acacagaagt caaaggaact gtcctcaaat gggagatcga gagcactccc
     1801 caaaacatat tttatgccaa gaggtttttc ctgctccatc gcttttgcgg cctcaataaa
     1861 agttggcgat atgtccaggg cagtcatgga ggcacccagc gaggcaaaaa tccttgtgtt
     1921 attgccttcg ccacagccta tgtccagccc ctcaagaccc ccaatttctg gaagcatggc
     1981 cacaaaaccc ggcgtattga actggtcacg acaaatgtcg tagccaagcc tggaaaggtc
     2041 cgtccacgcc ctggcgtttt catcccaata ttttccaact tccttgtcat ccatgcaact
     2101 tatgatatcc caaccataat cattaggtag tctcacgaat gagattttcc ttaatctcac
     2161 ttctctatgc ttcaaaaatg acccaaagcg ttgacaaatg taaacggaac acatatcaaa
     2221 atgcttgagg atctttgaaa atttcctcag gaggataaga gatgaaagaa aagacctgca
     2281 ctgtatgttc cgcatccgaa aaccacatga tgcctatgtc aatgctcagg aagccaatca
     2341 tagatcttat aaaaaaagac cacccggata ttaatgaaaa cggatacatc tgcaccgaag
     2401 atctgaacaa atacagggct aaatatattt ccagcctcct ggaagaagag aagggtgaac
     2461 tctccagcct cgaaaaacag gttgtaaaaa gcattgagga ccaggaaaat atcacaaaaa
     2521 acctaggtga acaaatcagc agaaaggtaa cttttggtga aaaaattgct gacaaggtgg
     2581 ccgattttgg cgggagctgg acttttgtca taatattttt tgcatcaatc ctgacatgga
     2641 tgaccataaa tatcatagcc ctcagaaacc cgtttgatcc atacccattt atattgctca
     2701 acctcgtcct ctcctgcgta gccgcaatcc aggccccggt aatcatgatg agtcagaaca
     2761 ggcaggaggc cagggacagg atgagagacg aaaatgagta tgcaataaac ctcaaggcag
     2821 aaatcgaaat aagacacctc caagacagac ttgaccattt catcatgaag gaatggcaaa
     2881 gactccttga gatacaaatc gtccagacag atcttataga ggaattagca gaaaaagatg
     2941 tattgggggg aaattttgga aagccaaccc gaaagtaagt aaaaggtgaa gtaatcaaaa
     3001 aaatcagata aattacaatt tgaatttaaa ctacataaac tgacacatat acaaccattt
     3061 aattaaacgg gaccctttgt aggctctcca caaaagaatt tatcaaagaa ggtttgtggt
     3121 cacaagacat ataagcgttt gcatttgttg aatcaaattg atacaatcgt attaggggat
     3181 ttttgcacaa aaagagaaag ggtttgtagt ggctagaaaa aaagaccaaa aaagatacta
     3241 ctacgaccac gagccatact acgcaaagat ttgttgcgag ggtggcactg ggtggccaat
     3301 tgaatctgat gtatctcaga ggttccttct caaatgggcc agggagcaaa tctttcctga
     3361 atatgccacc attctggagg ttggttgtgg tggcggggag cttgcaatcg agttggcaaa
     3421 acggggggta aaagttgagg cctgcgatct ttctgaaaca gcaataagga tatgccagtc
     3481 caaaaatcca ccagaaaacc tggctttctt tgctgcaaat gctatgacac acaagggata
     3541 tccaaaccct ccctacgact ggatcatagc caaccaggtc ctccaaagta tcataggggg
     3601 agacagggtt ttcctcctga aaagaatgcg cgagaacctc acaacaacgg gcacaatcgt
     3661 ggtggctaca atgcttgggg tccctgaaaa attcaaggaa gagtttaacc ctataacttt
     3721 cgtaaacaga acaaatacaa ggttttttgc tgatttgcgg atgttcgcaa aagaaattgc
     3781 agaagcaaga ctaagagtga cacgccatat aaaaatcgac gaccattaca ggatattttt
     3841 cctggagagg ttaccctgat aattccttct tggaatttcc tacaataatg agggcaaaat
     3901 ggacatagag cgcggcgcaa aaccagggga catcccagaa ctcataaaac tccatagata
     3961 tggcgaagaa cttcttgagg gcaatcgttc acagtttctc atggacccaa tgattatcac
     4021 ggatgaacaa ctcgaagacg atttcaacga atggcttgaa aatccggaaa ttggtttttt
     4081 tgtggcaaaa gagaacggaa aactggccgg ttttgttctg gcgatgataa tagaagaacc
     4141 ggacgatcta atcaatgttc catatgtgag tatcaacgaa ctggctgtga gcaaggaatt
     4201 ccagggttcc ggggttggaa ctaccctgat gaagagagtt gaggagtggg cactggaaaa
     4261 tgacataaat attgtccaac tgaatgtttg ggaattcaac cggaaggcaa aaaccctcta
     4321 tgaaaagctg ggtttcaaaa caatccagtg ccagatggaa aagattttga gcaaaagata
     4381 agagtttatt cccccgcaat cctcgcaaaa attaaaaaag gtcgcaagaa tcttgcgacc
     4441 tttttatttg ttgtgattaa aaacaatcaa tctatgaaag cgcctcttca ataactttcg
     4501 caaccttcga accatgggcc ctgccataaa gggactgcat tgcctggccc tttgcccacc
     4561 ttgtctgagc ctcttttgaa gagaacttga gctgcattga tctgtctaca acaccctcga
     4621 caatgaattt tattaccgag tctgttgcca ttgactgggt ctttgcaatg taattggaat
     4681 cggaagcaaa agccttgagc tcgttcttga ttgcttcttt tggcagaccc ttttccttgg
     4741 cttccatcat gacttttata acagtgtcat cagtcagcct ggagacaatc aatctttgtc
     4801 tcttgagatc agtgagccaa tctacaatca attctgcggc aagcttcggc aaaataccca
     4861 aatcaacaag cttgtcaaac agctttgctc tctggtgggt cgccagcttg tcggaaacgc
     4921 acttggaaag tcccagtttt gcatattttt cttcgcgctc ccatgggcgc tgggccatgt
     4981 ctgcccttat gcggtcaacc ctctccctgg tcaatctgac tggcggagaa tcggtgtccg
     5041 gatacatacg gtctggtcca ggcaagaccc tttcgaaatc ggtcctgtag ccatcttgca
     5101 tgtgttgcct tgtttcttcc ggtattcccc tgactgcctc gtctgccctc agtattactt
     5161 cattgacggc ggttttcagg tcagcctctg ggccccagac aagcactatt tcgtctgttg
     5221 gataagcatt cagtttttgt gcgatcggtt cccagtcacc aggataaagg agcttgcccg
     5281 atctgaaggt gagatttggc atcttgtcga tgcaggcgat gaccctgacc ctcgcgccaa
     5341 attcatcagc aaactgccaa ccgggctgga gctctgccga taatacttca tgaaactgcg
     5401 ggagcttgac tgcccaaatc ttgtcacccc ttgagatcgc atttttgacc tgcggggaag
     5461 aagatttcag gagcatgtct gtcacatctg ccacatttga atttcttatc ttttcaagct
     5521 cgatctggcg ctcctgctcc ctgagcgaag aatccagctt ctggtttgga acaaggccat
     5581 aagaaacaag tttttccttt atctcaagga gttttctctg cctcttcact tcagtgtcga
     5641 gcattttggg gatgtaccat gttttcggaa cacccttaat ttcgattcta ggtgctccat
     5701 ttatcgagct gttgacatcc tgcctgacag tccctatacc tcgcctgaca agccctgagg
     5761 ccctcaggag cctgccaagg agctcgtcga cctctgcaca ctcccggggt gttctcatgt
     5821 cgggataagt gactatctca atgagtgggg ttgagagcct gtcagttttg aaaactatcc
     5881 tgtggccaat atcggaaact tccctgcagg aatcctcttc aagtgccagt tgctgtatcc
     5941 ttatttttct gcctttgtat ggcacccagc catcaacacc aatgatggcg gttctctgga
     6001 aaccggttgg tatggaacca tccagatact gttttcttgt gatatggact tcgtccacca
     6061 gctggcagtt gagcatgagc gcgatttcga ttgcgtaatc aagtgcctgc tggtttatcg
     6121 ggaatggtgg cgtatcatcc atctcgtatg tgcaaacata atcacgataa agaaggtaca
     6181 caacttcttt gtgggttttg aactccatca gtgccgtgcc gtcgtatgtg cccatttcgg
     6241 agagcgttgg cctcatgtga cgaacaatcc tggcgtccgg ctccaccttc tggtagccaa
     6301 ccgggcagtg gcaaaacagc tttttctggg tatttagctg ttgatgaatc tcaagtcctg
     6361 atttgaaacc caggctttgg tagtaatcaa gatcgtcgta gttcatgtat acctccaaaa
     6421 atcggattct aaggttttga aggcaaaatc acaagaaaac ttctggtgtt tttatgagaa
     6481 acgcccaaaa attatttgtt cagctcttgt ttcaccttta aaaacagatc tttgttctca
     6541 ggccctattt cttttatctt tgcccacact tcactgcatg aactatcaat aagaccgatg
     6601 gacaatctta tggccatgtc caattttttt ctgtcctgtt tgttcaaagg gtcgagatca
     6661 gtctccacaa aaagatggtc aagatgtctc aggtagcaac tcatgttgtg atctcctttg
     6721 tcaaggttct agctctactc aggcaaaaat cacttgccaa gccatgcgtc ccagaaagca
     6781 tcccagtctg ccatgtcgat tttcccatcg gggacaaggt cagcaagcgg attccagaaa
     6841 tctggcgcct ctttgccttc ggcatttgta ccttctgcgc caaagcatgt ggccatttct
     6901 atagcatcgg cataatcaat atctccgtta agatcaaggt cggcaaatac agcagactca
     6961 aggaacctgg caatatggga gacagtcttg agtgttatct catctcttga tggccggata
     7021 tttttgccag aaaaaccatg gccaccgttt ttgaccttta tcaatgcaca agtaacattg
     7081 ttttcataaa gctttttggc aagcacgacc gattgtgagt atggaacggt gagatctttg
     7141 ttgccatgct ggattagtat tggtggatca tttttatcag cataggtgac cggcgatgcc
     7201 tgggttgcct tgtctggaca atcggagggg tagcacccaa gaaaatcctt tatgagctcc
     7261 acgcctggga gttttttgtc attgatttct tttgtcataa aagtcaggtc agacgggcca
     7321 aaatagtcaa caacgcaaga cactctgctc gaaattcctt caagtcccgg cccttcgagc
     7381 ccatcgccat catttgcaag tcccatcagc gcaaccagat ggccaccggc cgaaccgcca
     7441 tgtattccaa tcctgtcagg atcaatccca aatttctccg catttgccct g
//
LOCUS       DAOSRI010000049         7394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9357_length_7394_cov_4.174683, whole genome shotgun sequence.
ACCESSION   DAOSRI010000049 DAOSRI010000000
VERSION     DAOSRI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_7394_cov_4.174683"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="thyX"
                     /locus_tag="PK717_02410"
     CDS             complement(<1..1152)
                     /gene="thyX"
                     /locus_tag="PK717_02410"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOR46548.1"
                     /translation="MSNATRLVLLSFTPDPQRICSSAARLSSLPGSPTKILDETPEGG
                     YPDYLRRILSMGHHSVMEHANFTVAFENVSVFVEQFVIGFRLFSFTVKSRRYVPHDEA
                     GYVVPYMAPEGGPDLFGSDDKPYIGPEFYGPLSQRYLHFCEGLFMAYKRLLELGIPAE
                     DSRFVLPYSFKSNFIVTGNARSWIHLIHSAKNGLGKNYSEIHWVGTKLQEMLSKVAPA
                     FFTRIDDIERLSYEKDQRLALVSSKLGLEKNYSEEKVQLLWHTPDPDRAVAVSALMGA
                     SRATASQIEKALTKEAEVETIRAVCENGHARELEQAIFTFRLSGMSLPMLTHLTRHRM
                     QNIIVPTLVQAGKSPEIICPSTVGNNPEALAVFNSAVSSQRSFWEAMERF"
     gene            complement(1153..1707)
                     /locus_tag="PK717_02415"
     CDS             complement(1153..1707)
                     /locus_tag="PK717_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR46549.1"
                     /translation="MPTREDALTLVKSKVDNKNLVKHMLSAEAIMKALAKKFGEDEEL
                     WGLAGLVHDVDYVQTEKDPKNHAKIGAKLLSELGYPKEVSQAVMAHNPENGSKMETLM
                     DKALYVSDPLTGLIVACALIHPDKKLSSIDTEFVLSRFKEKRFAAGANREQIASCCGI
                     GLSLEEFVLLGLEAMKGISKELGL"
     gene            1988..2530
                     /locus_tag="PK717_02420"
     CDS             1988..2530
                     /locus_tag="PK717_02420"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOR46550.1"
                     /translation="MAVKRVLRGTTVMLRPMVEQDLTTRAHWLLDPEIRQSLGLPDRP
                     SYTYQTALADSHRWLSDRKSSGDLLWAIDVDGKLIGDVTAHITDFERKGEMVISIGDK
                     AMWGKGYGKEACSLVLDEMFRESEIYYVDTYVVLGNNRSFSLALSLGFTPVGTQTGGT
                     RVLRLIRKAWMARRKAELAS"
     gene            2655..>7394
                     /locus_tag="PK717_02425"
     CDS             2655..>7394
                     /locus_tag="PK717_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46551.1"
                     /translation="MGRKFFAFLLSFIISIQFVVFPGNSLSEECRTGFQRGNLARTGC
                     YPDSCAPATDEVSLGWSYQADSSSSASTNIAPVALDNMVFLAVNNDDVTGSRGYIYAI
                     NPQNGTLVWEEKSTDKTEQFIVAGGRLIALRNGGIHSFDYSNGTKMWSYSPTDKEYDQ
                     INGSPLVNSGKIYATSSEGYFYCLDFANGKKIWEHASDETIITWHSISDGKIYYGTHD
                     PSVIHCIDQFKGSQIWRSEMDFHFYENTYPIIDQGKIYLIGHDEEDGEPYLYCLSTDK
                     CKKIFSFPVEGRELLNPVIVEGKIYLATKDGGIKCLQANNGNMIWEKDYNGAILCSPT
                     SSNGRLFFVAGKSLIVASCTDGNILYEEEIGVQSNIDVIPSMGRLFVLTKNARLLSFA
                     SQGKISIDKFAIRFGLLEANKNSSKDIKIKNNGDENIEMTAVKTGPVTLSSEKFTVAP
                     KSSYKLTVTPNLDLAKLGQSNGEIALSWNDGSKKIPFTFCKIAGDSFTYANDRSNVEG
                     KKYVPANQLPKRTILKKIWDGNPSIHSNKFWDKSPDAYWNGFFYKFVDTNISVIDPES
                     GKIVLERDLGKKIEKFYFDGDKMFLDASYWIFCIDPYTLKIIWSQTKRQDSQSICTKN
                     GKIAIESSRSGSVKGTQNIYIDIFDENTGKKTYNDKIILKETGPIEYGVAINLNLLYY
                     SADRLYCMNINTGKIIWKTGSFGGDPIIMPEGVICNAAETGKKETFVRCSDPGTGKKL
                     WLFEGKLLCVGNKRVISEKNGQLSCLDASNGKLLWSKPLDDNTLESVMIMGNSVVMSN
                     GKKMLYFDISSGNLTGSSEMVGIPVCAGGGKIIIGDLIDISFQKTYTPYCYRDFDSVE
                     INTPSLDFGTELSKISFELTNYTGKTKNITTEADDGSISFKPSQFSLEPEKKIQVEAT
                     IGRVDTTPGTKVVPVRIKWDDGSIDLNAISYKDAGLPSWPNEDGDGGGRTPTSSNLDL
                     NKLTLLWKYGPGNNEESNKDIYGFKVPTGVLCGDGKLFTYDKITDDEKSSNIIRKIHY
                     LRCFDSSSGKVDWEKEFYTENMVWCDGKLYAKTGNTLTCLLGATGETLWSNFVPDSSN
                     FMVYDGLLYSKNGCYDTRDGKKLWETKTNEFAVGNGKFFSYYYYGDYKDRKTLLTCLD
                     ARTGKKIWENDISKLDIYNSNIFYTNGILICGNTLGIDGTTGKILWKTFISYGGCYDT
                     PSIFAANGFFVTISTICMDFFLNKAGAEDYYDIHRIPYHHVNIVDVFNGKIIRKLEFR
                     ATSYPMIARDKIYFLSAMNLFGFNTKSGSFQEDSIPNWRFWTPNGFGLGLIACAGDKL
                     FVVKANGSVYCYSSGDGFLLDENFYDFRTVAPGTKKDIWATIENLSKNQVKMTIEVTT
                     PSVTAETNELTFEPGEKKKLKLTMDLSGNTSPENGKVLKGELVIRWPGLEKRLQLRGY
                     VENKKPEMTCADSFLENELNHLFIYPEACGPKKNLKKVMAWPDAYLTKDKVVALNNGA
                     LENYSLDGLKKTIFIKSPECKTLGTPDHEGAFYVADGKQVHKYDEVKGKKLWTAKNFK
                     MDEEMIIDEIYCYKGKVFVFVGTLYCLD"
BASE COUNT         2300 a         1477 c         1671 g         1946 t
ORIGIN      
        1 gaacctctcc attgcctccc agaacgatct ttgtgaagaa acagcggaat tgaaaacggc
       61 cagtgcctct ggattgttgc caactgttga gggacaaata atttctggtg attttcctgc
      121 ctggacaagg gttggcacta ttatgttctg catcctgtgg cgggtcaggt gggtgagcat
      181 cggcaagctc atgccggaaa gcctgaatgt gaagatggcc tgttctagtt cccttgcatg
      241 gccattctcg cagaccgccc ttatggtctc aacttcggct tcttttgtga gcgctttttc
      301 aatttgtgag gccgttgccc tggatgcacc cattagcgca gaaactgcaa ccgccctgtc
      361 cgggtccggt gtatgccaga ggagctggac cttctcttca gaatagttct tttcaaggcc
      421 aagttttgat gacacaaggg caagcctctg gtctttctcg tagctcagcc tttctatgtc
      481 atcaatcctt gtgaagaacg ctggcgccac ctttgaaagc atctcctgga gtttcgttcc
      541 gacccagtgt atctctgaat agtttttgcc caacccgttt tttgccgaat ggatcaagtg
      601 tatccatgat ctcgcatttc cggtaacgat gaaatttgac ttgaaagaat atgggagcac
      661 aaacctggag tcctctgccg gtatgccaag ttcgaggagt ctcttatagg ccatgaaaag
      721 cccttcgcaa aaatgcaggt atctctggct caatgggcca tagaattctg gcccgatgta
      781 tggcttgtcg tcactgccaa aaagatcggg gccgccttct ggtgccatat agggaacaac
      841 atatccagcc tcgtcatgcg gaacataccg cctggatttg acagtgaatg aaaaaagcct
      901 gaaaccgatt acaaattgtt ccacaaagac cgatacattt tcaaatgcca ccgtaaaatt
      961 ggcatgctcc atcactgaat ggtggcccat cgagagtatg cgcctgaggt agtccgggta
     1021 gccaccctcc ggtgtctcgt cgagtatttt tgttggggaa cccggaaggc tggaaagcct
     1081 ggccgcgctg gaacaaatcc tttgcggatc aggtgtaaaa gaaagcagga ccagccttgt
     1141 ggcattgctc atctatagcc ccagttcctt gctgatgcct ttcattgcct caagccctag
     1201 caacacaaac tcttcaaggg aaaggccaat tccacagcaa gaagcaatct gctccctatt
     1261 tgcaccagca gcaaagcgct tttctttaaa cctgctgagc acgaattccg tgtctatgga
     1321 tgaaagcttt ttatctggat gaatgagcgc acaagcaact atcaggccgg taagtggatc
     1381 tgaaacatac aacgctttgt ccatcaaggt ttccattttg gaaccattct ccgggttgtg
     1441 cgccattacg gcttgggaaa cttcttttgg atatcctagt tcagaaagca atttggcgcc
     1501 tattttggcg tgatttttgg ggtctttttc agtctggaca taatcgacgt catggacaag
     1561 cccggccaaa ccccacaact cctcatcttc accaaatttt tttgccaggg ccttcattat
     1621 ggcttcggct gaaagcatgt gtttcacaag atttttattg tcaactttgg atttgaccaa
     1681 agtgagcgcg tcttctcttg tcggcatttt acaccttcct gttacgtgaa gtttagcaga
     1741 tggagcctgc cggtcaagaa cttttgctgt gtattttttg cacgaaaatc acatataaaa
     1801 gtgcattttc tcaaaaaatt catattttac ttttatagtc aaaagaaagc tttttcttgc
     1861 aaggttcgaa tccataatac aataaacaat ttttgttcaa tttttggtta aataaaccta
     1921 ttgacaagcc cataaatcat cttaacttgt aaaaaagggg aagtgtaacc taagaaagga
     1981 aagtgcgatg gccgtaaaga gggttcttcg tggaacaact gttatgctgc ggcccatggt
     2041 cgagcaggac ttgaccacaa gggcacactg gctgcttgat ccagagatca gacaaagcct
     2101 tggtttgcct gatcgtccca gttacactta ccaaaccgca cttgcagaca gccacagatg
     2161 gctttctgac cgaaaatctt ccggtgatct tctctgggcc attgatgtgg acgggaagct
     2221 cataggcgac gtaactgcac acataactga ttttgagcgc aagggcgaga tggtcatatc
     2281 catcggcgac aaggcaatgt ggggaaaagg ctatggaaaa gaggcctgta gtcttgttct
     2341 ggacgagatg ttcagggaat cagaaatcta ttacgtagac acatacgtag tgcttgggaa
     2401 caacagaagc ttctctcttg ccctctcgct gggctttaca cctgtgggga cacaaacagg
     2461 cggcacaagg gtgctcaggc tcatccggaa ggcctggatg gcacgccgca aggccgagct
     2521 ggcaagctag cattcccgtc ataaaaacaa ataaatctat ctatccgcag atatttttta
     2581 ggtttgcatt tttggtgaat aattcataaa ctcccttata gaggacaaaa aaataattgg
     2641 gctaggaggg ttttatgggc aggaaattct tcgctttttt gctttctttt ataatttcaa
     2701 tccagtttgt tgttttcccg ggaaattcac tatcagagga gtgcagaaca ggattccaaa
     2761 ggggcaatct ggcaagaaca ggctgttatc cggattcctg cgcacctgcg accgacgagg
     2821 tctctctggg ctggtcctac caggctgatt ccagctcatc cgcaagcaca aatatcgcgc
     2881 cagttgcgct tgataacatg gtatttctag cagtaaacaa tgatgatgta acaggaagca
     2941 gaggatatat ctatgctatc aatccacaaa atggaactct ggtctgggaa gaaaaatcta
     3001 ctgataaaac ggagcaattt attgtagctg ggggaagatt aattgcatta cgcaatggag
     3061 gaatccactc cttcgattat tcaaatggta ctaaaatgtg gagctattcc cctacagata
     3121 aggaatatga tcaaataaac ggttcgccac tcgtcaattc tggcaagata tatgccacat
     3181 caagtgaagg atatttttat tgtctcgatt ttgcaaatgg taaaaaaatt tgggaacatg
     3241 cttcagatga aacaattata acctggcatt ccatatccga tggaaaaatt tattatggta
     3301 cccatgatcc ctcggttatc cactgcatcg accaatttaa gggatcacaa atctggcgat
     3361 ccgaaatgga tttccacttt tacgaaaata cttaccctat aatagaccaa gggaaaatat
     3421 atcttatcgg ccatgacgaa gaagacgggg agccttattt gtattgcttg agcactgata
     3481 aatgcaagaa gatattttca tttccggtag agggcaggga actcctaaat cctgtaatag
     3541 tagaaggtaa aatttacttg gcaacaaaag atggtgggat caagtgtttg caagcaaaca
     3601 atggaaatat gatttgggaa aaagattaca atggagcaat cctgtgttcc ccaacttcat
     3661 caaatggcag gttatttttt gttgcaggca aaagcctgat tgttgcctca tgcacagatg
     3721 gtaatattct ttatgaagaa gagattgggg ttcaatctaa tattgatgta attccgtcga
     3781 tggggagatt gtttgttttg acaaaaaatg caagattatt atcttttgct tctcaaggca
     3841 aaatcagtat tgataaattc gcaataagat ttgggctttt ggaagccaat aaaaactcat
     3901 cgaaagacat caaaataaaa aacaacggtg atgaaaacat cgagatgaca gcagtaaaaa
     3961 cagggccagt tacgctctca tcagaaaaat ttactgttgc cccaaaatca agctacaaat
     4021 tgacggtcac cccaaacctg gacctggcaa aactcggcca gtcaaatggg gagatagcgc
     4081 tctcctggaa tgatggctct aaaaaaatac cattcacttt ttgtaaaatt gcaggggatt
     4141 cttttacata cgcaaacgac aggagcaacg ttgaaggcaa aaaatatgtg cctgcaaacc
     4201 agcttccaaa aagaacgata cttaaaaaaa tatgggatgg caacccaagc atccactcga
     4261 ataaattttg ggataagagt ccagatgcat actggaacgg attcttttac aaattcgtgg
     4321 ataccaatat atcagtgata gacccagaaa gtggcaaaat agtattggaa agagaccttg
     4381 gaaaaaagat agaaaaattc tattttgatg gcgacaaaat gtttctggat gcaagctatt
     4441 ggattttttg tatagatcct tatacgctca agatcatttg gagccaaacc aaaaggcagg
     4501 attcacaatc tatatgcacc aaaaatggga aaattgccat cgaatcaagt cgtagtggta
     4561 gtgttaaagg aacccagaat atatacattg atatttttga cgagaataca ggcaaaaaaa
     4621 catacaatga caaaataatc ttaaaagaga ctggaccaat tgaatatggg gtagccataa
     4681 accttaatct tttatactac agtgccgaca ggttatattg catgaacatc aatacaggaa
     4741 aaataatctg gaaaacaggg agttttggcg gggatccaat cattatgccc gaaggggtaa
     4801 tctgcaatgc agcagagact ggcaaaaaag aaacttttgt aagatgctcg gatccaggca
     4861 ctgggaaaaa attgtggctt tttgagggta aactgctgtg cgtcggcaac aagagagtca
     4921 tttcagaaaa aaatggccag ctgtcatgcc tggatgcgtc aaacggaaaa ctgctgtggt
     4981 caaaaccact tgatgacaac acactggagt cagtcatgat aatgggcaac agcgtggtca
     5041 tgagcaatgg caaaaaaatg ctctattttg acatttcaag cggaaacctg accggttctt
     5101 ctgaaatggt tggtattccg gtttgcgctg gtggagggaa aataattatt ggcgatttaa
     5161 tagatatcag ttttcaaaaa acatacacgc cctattgtta cagagacttt gacagtgttg
     5221 aaataaatac tccctcgctg gattttggaa cagaactctc aaaaatctcg tttgagctca
     5281 caaactacac aggcaagaca aaaaatatca cgacagaggc agatgatggc agcatctctt
     5341 tcaaacctag ccaattctcc ctggaaccag aaaaaaagat ccaggtcgag gcaacaattg
     5401 gtagagttga taccaccccg ggaacaaagg tggtcccggt aagaataaaa tgggacgatg
     5461 gctcgataga tctcaacgct atctcataca aagacgccgg cctgccatca tggccaaacg
     5521 aagatggcga cggaggcgga aggacaccca caagctccaa tctggaccta aataaattga
     5581 cattattgtg gaagtatgga ccaggtaata atgaggaaag taataaagat atttatgggt
     5641 tcaaagtacc aactggggta ttgtgcggcg atggaaaatt gttcacttat gacaaaatta
     5701 ccgatgatga aaaatcttcc aatatcataa gaaagattca ctatttaaga tgttttgatt
     5761 catcaagcgg caaagttgac tgggagaagg aattctatac agaaaatatg gtttggtgcg
     5821 atggaaaatt gtacgctaaa acaggaaaca cattgacgtg ccttttaggg gcaacgggtg
     5881 aaacactgtg gagcaatttt gttcctgata gctccaattt catggtgtat gacgggttgc
     5941 tctattccaa aaatggctgt tatgacacaa gggatggaaa aaagctgtgg gaaactaaaa
     6001 ccaacgagtt tgctgttggc aatggaaaat ttttttcata ttactattat ggtgattaca
     6061 aagatagaaa aacactgctg acctgcctgg atgccaggac tggaaaaaag atctgggaaa
     6121 atgacatatc aaaattagat atttataatt cgaacatttt ttatacaaat ggtattttga
     6181 tatgtggcaa cactttgggg atagacggga caactggcaa gatattgtgg aaaacgttta
     6241 tttcttatgg gggatgttat gatactccat caatatttgc cgcaaatgga ttttttgtaa
     6301 caattagcac aatatgtatg gattttttcc taaacaaagc tggagcagaa gactattatg
     6361 atatacacag gattccttat caccacgtta atattgtaga tgttttcaac gggaaaatta
     6421 taaggaaact ggagtttagg gcaacaagtt acccaatgat tgccagagac aaaatctatt
     6481 ttctcagcgc aatgaatttg ttcggtttca acacaaaatc aggctctttt caggaagact
     6541 ccataccaaa ctggagattt tggaccccaa acggatttgg attaggactg atagcttgtg
     6601 ctggtgacaa gctctttgtg gtaaaagcca atggaagcgt ttattgctat tccagcggtg
     6661 acggattctt gctggatgaa aacttttacg atttcaggac ggttgcccca gggacaaaaa
     6721 aagatatctg ggcgacaatt gaaaacctct caaaaaacca ggtgaaaatg accattgagg
     6781 tcacaactcc atcggtgacg gcagaaacaa acgagctcac atttgaacct ggcgaaaaga
     6841 aaaaacttaa actgacaatg gacctttcgg gaaacacatc gccagagaat ggaaaggtgc
     6901 tcaagggaga actggtaatc aggtggccgg ggttagaaaa acgattacag ctgaggggtt
     6961 atgttgaaaa caaaaaacca gaaatgacat gtgcagattc tttcctggaa aatgagctca
     7021 atcatttatt catttaccca gaggcatgcg ggccaaagaa gaacttgaaa aaagtgatgg
     7081 cctggccaga cgcttatctc acaaaagaca aggttgttgc attaaacaat ggcgccctgg
     7141 aaaactacag ccttgatggt ttgaagaaaa caatattcat caaatcaccg gaatgcaaaa
     7201 cacttggcac cccggaccat gaaggtgctt tttatgttgc agatggaaaa caggtgcaca
     7261 aatatgacga ggtgaaaggc aaaaagctct ggacagcaaa aaacttcaaa atggatgagg
     7321 aaatgatcat tgatgaaatc tactgctaca aaggaaaggt ctttgttttt gttgggaccc
     7381 tttattgcct ggat
//
LOCUS       DAOSRI010000050         7383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9373_length_7383_cov_3.970797, whole genome shotgun sequence.
ACCESSION   DAOSRI010000050 DAOSRI010000000
VERSION     DAOSRI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_7383_cov_3.970797"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1712)
                     /locus_tag="PK717_02430"
     CDS             complement(165..1712)
                     /locus_tag="PK717_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOR46552.1"
                     /translation="MSDKKTIYTPDDLANFDYGRDLGNPGSYPYTRGVYPTMYTERPW
                     TMRQYAGFSTAKETNERFKLLLQKGQTGLSTAFDLPTQMGYDSDHEMALGEVGKVGVA
                     ISTVDDMAELFDGIPLEEISTSMTINASATVVLAMYMKIAKNQGGDVSKLSGTVQNDI
                     LKEYIARGNYIYPIKPSMRLNCDLIEWASFKLPKFNTISTSGYHIREAGATAVQELAF
                     TFADAFAYIDDLTSRGLSIDDFAPRVSFFFASHNNFLEEVAKFRAARRIWAKLLEKKY
                     QPKNPKSMLLRFHTQTGGSTLTAQQPEINIVRVAFQAMAAVLGGTQSLHTNSFDEALA
                     LPSPKSATIALRTQQLVAFESGVCDVVDPLAGSYYVESLTNRIENDVYDYLDRIERMG
                     GTLACLEKGFFQSEIAKSAYQFQMDVESGKQAVVGVNKFTTSEEPKVDISRSGVDENE
                     RRAKLKAYKESRDQDKLKEAMGALKEVATGKGNLMEPIIQCVDAQATLGEICCMMREI
                     YGEYQEG"
     gene            complement(1705..2643)
                     /gene="ribF"
                     /locus_tag="PK717_02435"
     CDS             complement(1705..2643)
                     /gene="ribF"
                     /locus_tag="PK717_02435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOR46553.1"
                     /translation="MKVIKGDPALQVKTQTPGACVMIGYLDGVHVGHQMLAQKVIELA
                     KEKNGYPSAFTFNGKLYTKNIPSRGMLTTSHEKIELLEELGIDTVFLADFTDWLRDMS
                     DEDFVRIILKDNIKASAVAVGPSFTFGRGGKAGTKELRNLCLKNGIDCTIIPQVTIGD
                     EPISSSRIRNYLYAGNIFEANVLLGRRYFLTGTIVSGAMVGRSLGFPTANLDVSNPLK
                     LIPGGGVYACFGHLEGVKYECAVSIGNRPTFLGGDNSLEAHILDYSGLIYGRELKLEF
                     VAKIRDQIQFESKEKLSLQISKDVIKIREILEGQHE"
     gene            complement(2640..3527)
                     /gene="truB"
                     /locus_tag="PK717_02440"
     CDS             complement(2640..3527)
                     /gene="truB"
                     /locus_tag="PK717_02440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR46554.1"
                     /translation="MISGGLPINKEDGPTSFQVTNRAKGLYCASKAGHMGTLDPHARG
                     VLVVLFGASTKLIPYAKEHKKSYTAKFKLGFTSNTYDAWGQLTEHEYNSDTTKEHIEA
                     ILSQFIGKTEQIPPMFSAKKIEGTRLYELAMRGIELPRKPQPIEIDELKLNFWDQGSG
                     EGEFNLTCTKGTYVRSLISDIGIHAGCGAIMTALTRTSDQGFDLEQSVSISKIEEHVF
                     EGTHLNLIIPPEHFLSHLPEVQVSTRQQEILYHGNPVNLRSPTAHAGLAKIFGSKEKL
                     VGIGKYVPATGQLLPERML"
     gene            complement(3524..4507)
                     /locus_tag="PK717_02445"
     CDS             complement(3524..4507)
                     /locus_tag="PK717_02445"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR46555.1"
                     /translation="MNKVDLTTIAAELKKTKKVIILTHENPDGDAIGSALALSKAMQS
                     NGSKVIVFSPDNPPSSFDFLDGFNDIKIASDPEVIELVRKGYRIIVVDGSSPNRCGIS
                     NFPPGSDPIVIDHHPTIEDYGQMVFVDTTKSSTAENIFHILNAAGMQIGLTISECLFT
                     GIITDTGSFKYPNATSETFEITAQLLKSGMSTAKVSSMIYERMSYANRKLLGRAMERL
                     SVSFGGKFAITAVTWKDMNEFEAELRDTDFIIDEIRKLGGCEIYVFAKEYRPDQFRIS
                     MRGRGMFDLSQLALKYGGGGHHDAAGFNATGDWLFVRANLLQIFESLFDNK"
     gene            complement(4434..4844)
                     /gene="rbfA"
                     /locus_tag="PK717_02450"
     CDS             complement(4434..4844)
                     /gene="rbfA"
                     /locus_tag="PK717_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR46556.1"
                     /translation="MSDLRKKRLEHQIQEAVSWVLIRNIDNPKIGFVTITEVKVSVDF
                     RIATVYYSVLGSQKKIDETQAGLSSASKMIQKLVAEKIEFRQVPLLRFKFDETPSKAQ
                     KLEFTFRELENEQGGSDNDSRRTEENEESDHSDP"
     gene            complement(4841..5125)
                     /locus_tag="PK717_02455"
     CDS             complement(4841..5125)
                     /locus_tag="PK717_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HOR46557.1"
                     /translation="MPVGLIEITMRLFGVKSLKEKRSLVRPILARLRHDFNCAAIESN
                     AQDSHDVFVLSAVTINTSSIELGITLDNIFKTVEHESSLPCEIARRETFS"
     gene            complement(5126..5716)
                     /locus_tag="PK717_02460"
     CDS             complement(5126..5716)
                     /locus_tag="PK717_02460"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46558.1"
                     /translation="MVVKADSEGSLDAVKFALSKIKVKDIPANIVYSGIGVISDNDVL
                     LADASKAMLVGFNVQVDPLAKKTAKQNGIPINTYNIIFELTDAITEAIKRLIAPEYRD
                     VKVGQAEVRQVFNISDVGNIAGCFVSEGYVNHKVKVRVVRNRLPVYEGKISSLKRFKE
                     DTHEVKAGYECGVGIQGFNDIKVGDTLEFYTTQEVQ"
     gene            complement(5707..>7383)
                     /locus_tag="PK717_02465"
     CDS             complement(5707..>7383)
                     /locus_tag="PK717_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOR46559.1"
                     /translation="PKTKEPPKHETRPVEEKTPTKVTPAKEKEKPTVEKPVEHSEKLI
                     IDKTLNVPVSKYRSDQRPTGIQMPKRVEPVKLPPLPPQKQPLYPITPRSNFQRTPTSQ
                     RPPTGQRPPSSGQKPPMTQRPVIDKDREKQKYEHAIDHNKDKDKQQISNAPFIVGKEH
                     FVIKRTPPKSHRKTQQPKQAPKPVEVKPQKLTIDVFAKTAGVPEQRVMAYMLKQGYIP
                     NPNEELTGPVLEMLSSTFGLKTSHEGTLSDENLIPRSPIVTVMGHVDHGKTTLLDYIR
                     KTSVALGEAGGITQSIGAYKVVVNDRDIVFIDTPGHEAFATMRREGANVTDIVVLVIA
                     ADEGVKEQTIEALNMAKEAKVTIIVAANKMDRPGANLDKLYSQLAELGLTPEAWGGDT
                     MVAPITAKTGDGVKDLLEMILLQADILDLKMNKAGKLAGTIIESHMDRFVGPLATAII
                     QSGQLKAGDYIEAGNAWGRVKALFDHADKKIQKIQAISPVKIMGFDQVPKPGSILLAS
                     DISRRRKPEKGQVLETQPVPQMPTDEEAPSNLDDLFKVFDQQGATKLMWS"
BASE COUNT         1697 a         1652 c         1645 g         2389 t
ORIGIN      
        1 caagaagctc gactatcaga ccatcatatc tcagcttggc tattttggct ttctggtctg
       61 gtacttcagt gacatgtata agctgaaaac caagtaacaa ccatgatttc attgcttctt
      121 caatgtttgt cgttgctatt cctatgtgtg aaagacctgt catatcatcc ctcctggtat
      181 tcgccgtaaa tttcgcgcat catgcagcaa atttcgccaa gtgttgcctg tgcatcaaca
      241 cactggataa ttggttccat aagattgcct tttccagttg ccacttcttt taatgcaccc
      301 attgcctctt taagcttgtc ctggtccctg gattccttgt atgccttgag ttttgccctt
      361 ctctcgtttt catcaacacc gcttctgctg atgtcaactt ttggctcttc actggttgtg
      421 aatttattga cgccgacaac tgcctgtttg ccagactcta catccatctg gaattggtat
      481 gcagatttgg caatttcgct ctggaagaat cccttttcca gacaggccaa agtgccgccc
      541 attctttcta ttctgtcaag ataatcataa acatcgtttt cgatcctgtt tgtcaatgat
      601 tcgacgtagt aagaaccagc cagtggatca acaacatcac acacacctga ctcaaaagca
      661 acaagctgtt gtgtcctaag cgcgattgta gctgattttg gcgatgggag cgccagtgcc
      721 tcgtcgaaag agtttgtgtg gagactctgt gttccaccaa gcactgccgc catggcctgg
      781 aaagcaacgc gaacgatatt gatttctggt tgttgtgccg tgagggtaga gccgcctgtc
      841 tgggtatgaa aacggaggag catcgatttt gggttttttg gttggtactt tttttcgagc
      901 agttttgccc agattcttct tgcggccctg aatttggcaa cttcctcgag gaaattgtta
      961 tgtgatgcaa agaagaagga gactcttgga gcgaaatcgt caatcgagag acctctgctg
     1021 gttaggtcgt caatataggc aaaggcatcg gcaaaagtaa aagccaattc ctgaactgct
     1081 gtggcgccgg cttccctgat gtgataaccg gaagttgaga ttgtattgaa ttttggaagt
     1141 ttgaatgatg cccattcgat gaggtcgcaa ttgagcctca tggatggctt gattggataa
     1201 atataattgc ctctggcgat atattctttc aggatgtcgt tttgtactgt tccggaaagt
     1261 tttgagacgt caccaccctg gtttttggct atcttcatgt acattgccag aacgacagtt
     1321 gcggaagcat ttatggtcat tgaggtggaa atttcttcaa gcggtatgcc atcaaaaagc
     1381 tctgccatat catcaactgt tgatatggct acacccactt ttccaacttc gccaagagcc
     1441 atttcatggt cggagtcata gcccatctgg gttggtaggt cgaaagctgt ggaaagccca
     1501 gtctggcctt tctggagtaa tagtttgaaa cgctcgtttg tttcttttgc ggtggagaag
     1561 cctgcatatt gcctcattgt ccacggcctc tcggtgtaca tcgttggata aacgcccctg
     1621 gtgtaggggt atgaacctgg atttccgaga tctctgccgt aatcaaaatt tgccagatca
     1681 tctggcgtgt agatcgtttt tttgtcactc atgctggccc tccagaattt ctcttatctt
     1741 tatgacatct tttgaaatct gcaaagagag tttttccttg ctttcaaatt ggatttgatc
     1801 ccttattttt gccacaaatt cgagtttcaa ttctcttcca tatatcaatc cagaatagtc
     1861 aagaatgtgt gcctcaaggg aattatcgcc acccaagaaa gttggtctgt tcccaattga
     1921 cacggcacat tcgtacttta caccctcgag gtgaccaaaa caagcataaa caccaccacc
     1981 agggattaat ttgaggggat ttgaaacatc cagattggct gttgggaaac cgagcgatct
     2041 tccaaccatt gcgccagaaa ctattgttcc tgtcaggaaa tatcttctcc caaggagaac
     2101 gtttgcctca aagatgttgc ctgcataaag gtagtttctg atcctgcttg atgaaatcgg
     2161 ttcatcacct attgttacct gcgggatgat ggtgcaatct attccatttt ttaggcagag
     2221 gtttcttagt tctttagtgc cagctttccc acccctgcca aatgtgaacg atggcccaac
     2281 tgctacggcg cttgccttta tattgtcctt aaggattatc cttacaaaat cctcatctga
     2341 catgtccctc agccagtcgg taaaatcggc aagaaaaact gtatctatgc ccaattcctc
     2401 aagcaattcg attttttcat ggcttgtagt cagcattccc ctggaaggga tattctttgt
     2461 gtacaatttt ccattaaagg tgaatgcaga gggataacca tttttttctt tcgcaagctc
     2521 tataactttt tgtgcaagca tctggtgacc gacatggacc ccatcaagat aaccgatcat
     2581 gacacatgcg cctggtgttt gcgtttttac ttgcagggcg ggatcaccct ttatgacttt
     2641 catagcattc tctccggaag caactggcct gttgctggaa cgtatttacc tattccgaca
     2701 agcttttctt ttgatccaaa tatttttgcc agaccagcat gggctgtggg cgacctcaaa
     2761 tttacaggat taccgtggta aaggatctcc tgctgtcttg ttgaaacctg gacttctggc
     2821 aaatgtgaca aaaaatgttc aggagggata atgagattaa gatgtgtgcc ttcaaaaaca
     2881 tgttcttcga tttttgaaat ggaaacgctt tgttctaaat caaatccctg gtcacttgtt
     2941 cttgtcaatg ctgtcattat ggcgccacaa ccggcatgta ttccgatatc cgatatcagt
     3001 gagcggacat aggtcccttt tgtgcaggtg agattgaatt cgccttcccc ggaaccttgg
     3061 tcccaaaaat tcaatttcag ttcgtcaatc tctattggtt gcggttttct ggggagttcg
     3121 attcccctca ttgctagttc ataaagtctt gtgccctcaa tctttttggc agaaaacatt
     3181 ggtggtatct gctcggtttt accaataaac tgggacagta ttgcttctat gtgttctttt
     3241 gtagtgtcac tattgtattc gtgctctgtc agttgacccc aggcatcgta ggtatttgat
     3301 gtgaaaccaa gcttgaattt tgctgtataa ctttttttgt gttctttggc ataagggatg
     3361 agttttgtgg atgcaccaaa taaaacaaca aggacacccc tggcatgagg atcaagggtt
     3421 cccatgtggc ctgctttaga agcgcaatac agccctttcg ccctgtttgt tacctgaaag
     3481 cttgtcgggc catcttcctt gtttattggt agtccacctg aaatcatttg ttgtcgaaca
     3541 gtgactcgaa tatttgcagt aaatttgctc ttacaaaaag ccagtcgcca gtggcattaa
     3601 agcctgcagc atcatgatgt ccgccaccac catacttcag tgcaagctgg gaaaggtcga
     3661 acatgcctct tcctctcatg gaaatacgga actgatcggg tctgtattct tttgcaaaaa
     3721 catagatttc gcaaccaccg agttttctta tctcgtctat tataaaatct gtgtccctga
     3781 gttctgcttc aaactcattc atatccttcc aggtgacggc agttattgcg aatttacccc
     3841 caaaagagac ggaaagcctc tccattgccc ttccaaggag cttcctgttc gcgtagctca
     3901 tcctctcgta gatcattgag gaaacttttg ccgtcgacat tccgcttttt aacagctggg
     3961 cggtgatttc aaatgtttcc gatgttgcat ttgggtattt gaaggatcct gtatcagtga
     4021 tgattccagt gaaaagacat tcgctgattg ttaggccgat ctgcattcct gccgcattga
     4081 ggatatgaaa tatgttttct gcggttgagg attttgtagt atcgacaaag accatttgtc
     4141 cgtaatcttc aatggttgga tggtggtcga ttactatagg atcagagcct ggagggaagt
     4201 tcgatatgcc acacctgttt ggcgatgagc catcaactac aattatcctg tagcctttcc
     4261 ggacgagttc tatcacctcg gggtcgctgg ctatctttat atcattaaac ccatcgagaa
     4321 aatcaaaact ggatggagga ttatcagggc tgaagacgat aacttttgac ccattcgatt
     4381 gcattgcctt ggagagggca agcgccgacc ctatggcatc tccatcagga ttttcatggg
     4441 tcagaatgat cactttcttc gttttcttca gttctgcggc tatcgttgtc agatccacct
     4501 tgttcatttt ccaactccct gaatgtaaat tcaagttttt gtgcttttga tggcgtctca
     4561 tcaaacttga aacgcaaaag tgggacttgc ctgaattcaa tcttttcagc cacaagtttt
     4621 tgtatcattt ttgatgcaga cgagaggcct gcctgggttt catcaatctt tttttgtgat
     4681 ccaagcactg aataataaac agtggcaatt ctgaaatcta ctgatacctt cacttcagtt
     4741 attgtcacaa agcctatttt tggattgtcg atattcctga tgagcaccca ggaaactgct
     4801 tcctgtatct ggtgctcaag cctttttttc ctcaggtcac tcacgagaag gtctcccttc
     4861 ttgcaatctc gcatgggagg ctactttcat gctcgacagt cttgaagatg ttgtcgagag
     4921 tgattcctaa ttcaatgctg gaagtgttta tggtgaccgc agacaaaaca aaaacatcat
     4981 ggctgtcctg ggcgtttgat tcgattgcgg cgcaattgaa gtcatgcctt aatctggcca
     5041 gtatcggcct gacaagactc cttttctctt tcagtgattt cacgccaaaa agcctcattg
     5101 ttatctcgat caggcctacc ggcaattatt gcacctcttg cgtggtgtag aactccagtg
     5161 tatcgccaac cttgatgtca ttgaaacctt gaatgccaac gccgcattca taaccagcct
     5221 tgacctcgtg agtgtcttcc ttgaacctct tgagggagct tatcttgcct tcgtaaactg
     5281 gcaatctgtt tcttactacc cttactttta ctttgtggtt gacgtaacct tcggagacga
     5341 agcagcctgc gatgttgccg acatcagaaa tattgaagac ctgcctgacc tctgcctggc
     5401 cgactttcac gtccctgtac tctggcgcta tgaggcgttt tattgcctct gtaattgcat
     5461 cggtcagctc gaatattatg ttgtaggtgt ttattggaat gccattctgc ttggcggtct
     5521 ttttggccaa tggatcgacc tggacattga aacctacaag cattgctttt gaggcatcag
     5581 caagaagcac gtcgttgtca gagatgactc cgattccaga ataaacaata tttgccggaa
     5641 tatctttgac ctttattttt gaaagggcaa attttacggc atcgagagat ccttctgaat
     5701 ctgcttttac gaccacataa gttttgttgc gccctgctgg tcgaagacct tgaagagatc
     5761 atcaagattt gatggggctt cctcatctgt tggcatctgt ggaacaggct gtgtttcgag
     5821 aacttgaccc ttttctggtt tgcgtcttct tgaaatatca gatgcaagaa ggattgatcc
     5881 aggctttggt acttggtcaa agcccattat tttgacagga cttattgcct gtatcttttg
     5941 tattttcttg tctgcgtggt cgaatagtgc cttgaccctt ccccaagcat ttccggcttc
     6001 gatataatct ccagctttta gctgtccgct ctggataatg gcagttgcca gtgggccaac
     6061 aaacctgtcc atgtgggatt caattattgt tcctgcaagc ttgcctgctt tgttcatttt
     6121 caggtcgagt atgtcggcct ggagaagtat catttcaagg agatctttta caccatcacc
     6181 cgtttttgca gtgattgggg cgaccattgt gtcaccaccc caagcttctg gcgtcagacc
     6241 cagttctgca agctgggagt atagtttgtc gaggttagca ccaggacgat ccatcttgtt
     6301 tgcggccaca atgattgtta ctttggcctc ttttgccatg ttgagggctt cgattgtctg
     6361 ttcttttaca ccttcatctg cggcaataac aagaacaact atatcagtga cattggcgcc
     6421 ttctctcctc attgttgcaa aggcttcatg accaggagta tcaatgaaaa cgatatctct
     6481 gtcgtttact actaccttgt aggcgccgat actttgtgtt attccaccag cttctccaag
     6541 tgcaaccgag gtttttctta tgtaatcaag aagagttgtt ttgccatggt cgacgtgacc
     6601 catgactgtg acgattggtg agcgcgggat cagattttca tcagaaagtg tgccttcatg
     6661 agaagtttta aggccaaaag tagaagaaag catttccaaa actgggccag taagctcttc
     6721 attaggattt ggaatgtacc cttgcttgag catgtaggcc atgactctct gttctggaac
     6781 ccctgcagtt ttggcaaaga catctatggt gagcttttgt ggtttcacct caacaggttt
     6841 tggtgcctgt tttggctgtt gtgttttgcg atgactcttt ggtggagttc tcttgattac
     6901 gaaatgttct ttaccaacta taaatggagc gtttgatatc tgctgtttgt ctttgtcctt
     6961 gttatgatca atggcatgct cgtatttctg tttctctcta tccttgtcga tgaccggcct
     7021 ttgtgtcatc ggtggtttct ggccactgga aggcggtctc tggccagttg gcggtctttg
     7081 ggaagttggt gtacgctgga aattggatct tggcgtaatt ggatacagag gctgtttttg
     7141 tggaggaaga ggaggaagtt tgactggttc aacccttttt ggcatctgga tgcccgttgg
     7201 tctttgatct gatctgtatt ttgaaactgg aacattgagg gtcttatcta ttatgagttt
     7261 ctctgagtgc tcaaccggtt tttctactgt cggtttctct ttttcttttg ctggcgttac
     7321 ctttgtaggc gtcttttctt ctaccggcct agtttcatgt tttggtggtt ccttggtttt
     7381 tgg
//
LOCUS       DAOSRI010000051         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9462_length_7333_cov_4.252130, whole genome shotgun sequence.
ACCESSION   DAOSRI010000051 DAOSRI010000000
VERSION     DAOSRI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9462_length_7333_cov_4.252130"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK717_02470"
     CDS             <1..357
                     /locus_tag="PK717_02470"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOR46560.1"
                     /translation="SAHRTPDRTTQIAKEAMGKGHKVIICMAGYAAHLPGVVAALTNL
                     PVIGVPLSGSPLNGLDALLAQQMPSGIPVAAMTIGTAGAINAAVFAARIVALSDEAVR
                     TKLAQYVNDLAAKVLN"
     gene            427..1335
                     /locus_tag="PK717_02475"
     CDS             427..1335
                     /locus_tag="PK717_02475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR46561.1"
                     /translation="MRKKTARMLRRIVALSLFVCACILLVISAKLLFFPSVNEEPPKR
                     IEVFDRYWLRLQEKVDWLAKQRESKSAKDFLWLSKRTLELRPLSNRTVFLTFDDGPSG
                     TTPRILDILKANGVKATFFTLGLQVDDMPSIVRKTVEEGHCVLSHGYSHRYDLYNDPP
                     KFFEDIDKAMSSIMNATSKRPLPYVRLPGGANNQYPRKKPFDECKKGLINRGISYVEW
                     NASMDDSTFKKHTRNYMIENVLLKAKANNFIIVLMHDSAGKGLTISALPEVIKGLRDM
                     GFVFRTFSDLTDAERKRMLSLKLINR"
     gene            1478..1915
                     /locus_tag="PK717_02480"
     CDS             1478..1915
                     /locus_tag="PK717_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR46562.1"
                     /translation="MAIVRWNPMKELFWDDFGKEFRKMMRGEVEKVGEMGEFWAPSID
                     LSETQDAYALKADLPGMKSEDVNVEVHDDIVTISGERKEERSSEEENVHVTEVSYGKF
                     MRSVRLPISVDPDKVKAKFENGVLKVSLPKSEKVKPKKIEIEK"
     gene            1989..2762
                     /locus_tag="PK717_02485"
     CDS             1989..2762
                     /locus_tag="PK717_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOR46563.1"
                     /translation="MERIFVSQKLYLYPIEILDVPHRAMVRLKSLGVFRLEQLAGLTE
                     SDLLSVYHFGQKSLFELKRRLDILNQCVMEEGRPPEDIIECMRLKLEGPDEFLEEVIS
                     SFQEEDNGRTSICFYLAKLPPRGIFVVSERIGLRRAHIRTLQEIADVMSLTRERVRQL
                     QNRSLQYLQSSLCKEDILSDWEETLVWAATTPPPGEQSFLERIESTGVSGNANITGFV
                     VLATHIIKGIDLPPLLRAARDGKLNKIWARKHQTKNREE"
     gene            2863..3327
                     /locus_tag="PK717_02490"
     CDS             2863..3327
                     /locus_tag="PK717_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46564.1"
                     /translation="MIKKFSAVILIIFSVISLIVGAIYIWQGASKYSQITSMAAEEKV
                     QIGMTEDQIKNGNVVDTMSEMQKAADTIREHRRGIAKTYEELLGTGKFDPTNPKHATY
                     AQAINLENYLYVGMLSLGVSLSMIGIGLFMVMMAIAMLLTGLALFPEKAKLS"
     gene            3455..4696
                     /locus_tag="PK717_02495"
     CDS             3455..4696
                     /locus_tag="PK717_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOR46565.1"
                     /translation="MTRNKTSLALIFVLSLVAISPCLAISIEDQQDPKYCIVDTGQEK
                     CYDNSREIREPSRDGPFYGQDAQNKGNKPSYRDNGDGTVTDLNTGLMWVRERGKKVTW
                     KDAISGAPACRIGGYTDWRMPTIKELYSLINFTGRQARTESESVPYIDTKYFGFKYGS
                     GTGNERLIDCQDWSATEYLSTTMNNDASVFGVNFADGRIKGYPKQVRRREGAGDNRLY
                     ARYVRGNPEYGKNDFIDNGDGTVADRATGLVWSKGDSGTGMNWEQALKWVQKKNTEKY
                     LGHDDWRLPNAKELQSIVDYSRCPDRTDSPSIAPVFECTQIKNEKGDRDYPFYWTGTT
                     HLDNMGAVYVCFGRAMGFMEMPPGSGNYRCVDVHGAGAQRSDPKAGNPADFPKGRGPQ
                     GDVVRINNFVRLVRSGNKKIK"
     gene            4767..6530
                     /locus_tag="PK717_02500"
     CDS             4767..6530
                     /locus_tag="PK717_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46566.1"
                     /translation="MGRAEKIASWTKENRFFVVAGVLIVLCVIVVVPIFAQSLNRPDG
                     DYSTPALYLFSTIAQTMGAILGIVFAVFFAVYANMRSNPENPTIDYARSYLQSDRYFM
                     SAALVGLFCILGSVVCLLMIYASGSLLNITVMLLLGILCLVLGLVSITLLLYFVTVRI
                     PLYFEPIELYKEQRELLKKRSSISEKEYKRFLVDTLFISSADSNKYYLSEALLIIRCD
                     YKNDVSKVRDYYNLLRELNIAFTSRICKYPKENQYRIRLNIIETIKEATKYDPNNPDK
                     LNMPYNISYYRGDFFILLKFLVDNIAICFEENAVDKINIKKISKIFSEFTGNLSFYMI
                     NKQEKYIFVLSEFLNYLRCLQIANFRFYELIDLEALLPIVDDVIRYLFENNNIINKIN
                     DKLLENYIKYLCITFFNIIEYIEKTDYMIDLTKDELKTIFLYLEQIKKGFNNIYNCTE
                     ASLINREISLEEIIFKYSLCCKLFDFKYYKYILLYMLINLHKYYSKSFEKSYTYKSIY
                     ESINFPIGYAIYLCDLYENGIDSKIVDLDYRTYVDVFLKNFCEQNNLDFSKMEARFND
                     KFYLNKERFENIQKSYMRIIF"
     gene            complement(6507..>7333)
                     /locus_tag="PK717_02505"
     CDS             complement(6507..>7333)
                     /locus_tag="PK717_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR46567.1"
                     /translation="VLSMSSAAPLVKIASADTGPFSMAFWRLLIASIIMVPFGAPWRM
                     FHDKKTLLWSVLSGFFLAMHFAVWISSFHYISVSATAVLVTTAPIFIAMLEYIIWRKK
                     PTPFAILGIFLAIGGAAVIALWGTKMEGTMLGVVLSLLGALFGSCYLITSKIAQERAP
                     LLQTVSVSYPVASLLLLAGAFLTGEQLWGYSGASWLSIAGMALFPQVIGHTALNMGVK
                     YLTPIITTTTNLFEPVGASIIAWAFLGDPIGLNVLVGGLLIFAGVVVTGLKNNSHI"
BASE COUNT         2223 a         1448 c         1703 g         1959 t
ORIGIN      
        1 tcagcccata gaactccgga tagaacgacc caaatagcga aagaagccat gggaaagggc
       61 cacaaggtca ttatttgcat ggctggctat gcagcacatt tgccaggtgt tgttgctgcg
      121 cttaccaacc ttccggtgat tggcgtccct ctttctggat caccgctcaa tggccttgat
      181 gccttattgg ctcagcagat gccttcagga attccggttg ccgcaatgac cattgggact
      241 gcaggtgcca taaacgctgc tgtttttgct gccaggatag ttgccctttc tgatgaggct
      301 gtgaggacaa aattggccca gtatgtgaat gatcttgcag caaaagtgct aaactaataa
      361 aaagattgct aggtaatacc aaaattattg attattattg cctagctgga taagattggg
      421 gtcgtgttga gaaaaaagac ggcaagaatg ttgaggagga ttgtcgccct atctttgttt
      481 gtctgcgcat gtattttgtt ggttatctct gcaaagttgc tcttttttcc aagcgtaaat
      541 gaagaacctc cgaaacgcat tgaagtcttt gataggtatt ggctgaggct tcaggagaag
      601 gttgactggc tggcaaagca gcgtgaatca aaaagtgcaa aggattttct ctggctctca
      661 aagaggacct tagagttgag gccactttcg aatagaacag ttttcctgac atttgatgat
      721 ggaccgtcag gcacaacgcc tagaatactg gatattctta aagcaaatgg tgtaaaagca
      781 acctttttta cgcttggctt gcaggttgac gatatgccgt caattgtcag aaagaccgta
      841 gaggaggggc attgtgtcct ctcccatggg tatagccaca gatatgacct ctacaacgat
      901 ccgccaaaat tctttgagga cattgataag gcgatgtcct cgataatgaa tgccacaagt
      961 aaaaggccgc tgccttatgt caggcttccg ggtggtgcca acaatcagta tccaaggaaa
     1021 aaaccctttg atgaatgcaa aaaagggttg ataaatcgtg gtatcagtta tgttgaatgg
     1081 aatgccagca tggatgattc aacattcaag aaacacacca gaaactacat gattgagaac
     1141 gtgctactca aggcgaaggc aaacaatttt ataatcgtcc tgatgcatga ttctgcagga
     1201 aaagggctaa ctatcagcgc ccttcccgag gtgattaagg gactcagaga catgggtttt
     1261 gttttcagga cattttctga ccttacggat gctgaaagga aaaggatgct ttccctcaag
     1321 ctcatcaaca ggtgaccttc tctttacaaa aatatttcta cccttcaaaa atcagctata
     1381 cataattctt taaaacttgc cacttgaaat tttgtcaaga gggtgtataa gtgcattgta
     1441 tgaagaaaaa tagggaaagg aggaccaaaa cggtagtatg gcgatagtaa gatggaaccc
     1501 gatgaaagaa ttgttttggg atgattttgg aaaagaattc aggaagatga tgaggggtga
     1561 agtggaaaaa gttggtgaga tgggcgaatt ttgggcacca tccattgatc tttccgaaac
     1621 ccaggacgcc tacgccctga aggccgattt accaggaatg aaatccgaag atgttaatgt
     1681 cgaagtgcat gatgatatag tgacaatttc cggcgagagg aaagaagaga gaagctctga
     1741 agaagagaac gttcatgtaa ctgaagtctc ttatggtaag ttcatgagat cagtcaggct
     1801 cccgatctcc gttgatccgg ataaggtgaa agcaaaattt gagaatgggg tcctcaaggt
     1861 gtcccttcca aagagcgaaa aggtgaagcc gaagaagatt gaaattgaga aataagtgtt
     1921 gaagaacaag ccaactattt tcaggaggtg ggccggcgtc ccttgaatgg gcaccggcca
     1981 ttttgcgtat ggaaagaatt tttgtttctc aaaaacttta tttgtatccg atagagattc
     2041 ttgatgtccc ccatagggca atggtcagac taaaatcgct tggcgttttt cgccttgaac
     2101 aactggccgg tcttaccgaa tctgatcttc tctccgtcta ccattttggc cagaaatccc
     2161 tgtttgaact caaaagaagg cttgatattc ttaaccagtg tgtaatggag gaaggccgtc
     2221 caccagaaga catcatagaa tgcatgagac tgaagctgga aggtcctgat gaattcttgg
     2281 aggaagtgat atcatcgttt caggaagaag ataatggcag gacaagcatc tgtttctatt
     2341 tggcaaaact ccctccaaga ggcatttttg ttgtttcgga aagaattggc ctgaggcggg
     2401 cccatataag gacacttcag gaaattgcgg atgtcatgag tcttaccagg gaacgggtaa
     2461 gacaactcca gaatagatct ttgcaatacc tccaatcgag cctttgcaaa gaagacatac
     2521 tttcggattg ggaagaaacc ttggtttggg ctgccacaac accacctcct ggtgagcaga
     2581 gtttccttga gaggatcgag tccacgggtg taagcgggaa cgcaaacata accggtttcg
     2641 ttgttcttgc cacccacatc atcaagggga ttgatttgcc tccacttctt cgtgccgcaa
     2701 gggacggaaa actcaacaag atctgggcca gaaagcacca gacaaaaaac agggaagaat
     2761 aaaaattatt cgactggctt gccttggact attgccttgg cctcctaaat agttattatt
     2821 ttcacaacac gcaccgaagg tgccaaagga ggaaaagggt aaatgataaa gaagttttca
     2881 gcggtgatcc taatcatctt ctcggtaatc agccttattg ttggtgcgat ttacatctgg
     2941 cagggtgcat caaaatacag ccagataacc agcatggctg ctgaagaaaa agtccagatt
     3001 ggaatgactg aggatcaaat caaaaatggt aatgtcgttg acaccatgtc ggagatgcaa
     3061 aaggctgcag acacaatcag ggagcaccga aggggtattg caaagaccta tgaggaactc
     3121 ctcggaactg gcaaatttga ccccacaaat ccaaagcacg ccacatacgc ccaggcaatc
     3181 aaccttgaaa actatctcta tgttggaatg ctctcccttg gcgtttcact ctcgatgata
     3241 ggcatcgggc tctttatggt gatgatggcc attgcaatgc ttctgacagg gcttgcgctt
     3301 ttcccggaaa aagccaaatt gtcctgacaa aatcgcaaaa agcctgcctt gttttcccca
     3361 ggcaggcttt tttatttttt ttgaaaatcg ctgaccaaaa tacaattttc ttcgtacaat
     3421 tagccaaatg gattttttgt ccggaggtca taaggtgact agaaacaaaa cttctctagc
     3481 acttattttt gttctttcgc ttgttgccat ttcgccctgc cttgcaataa gcattgaaga
     3541 ccagcaggac cccaaatatt gcatcgtgga cactggccag gagaaatgct acgacaacag
     3601 cagggaaatc agggagccat ccagagacgg cccattctat ggccaggatg cgcaaaacaa
     3661 aggcaacaaa cccagctaca gggacaatgg cgacgggaca gtcacagacc tgaacaccgg
     3721 cctgatgtgg gtcagggaaa ggggcaagaa agtaacttgg aaagatgcaa tttctggtgc
     3781 tcctgcctgc cggattggcg ggtacaccga ctggcgcatg ccaacaatca aggaattgta
     3841 ctcgctgata aatttcacgg ggcgacaggc cagaacagaa tctgaatcag tgccctacat
     3901 cgacaccaaa tattttggct tcaaatatgg cagcgggacg ggcaatgaac gcctgatcga
     3961 ctgtcaagac tggtctgcaa ccgaatatct ttccacaaca atgaacaacg acgcctcggt
     4021 ttttggtgtc aattttgcag atggccgcat caagggctat ccaaagcagg tgcgcagacg
     4081 ggaaggtgct ggtgacaaca ggctctatgc cagatatgtc cgtggaaacc ccgaatatgg
     4141 caagaatgat ttcatcgaca acggtgatgg cacagtggca gacagggcga ctggccttgt
     4201 gtggtcaaag ggtgacagtg gcacaggaat gaattgggaa caggccctca aatgggtgca
     4261 gaaaaagaac accgaaaaat atctgggtca tgatgactgg cggcttccca acgcaaagga
     4321 actccagagc atcgtcgatt attcaaggtg cccggacaga acggactcgc cttcgattgc
     4381 cccagtcttt gaatgcacgc agatcaaaaa tgaaaagggc gacagggact acccgttcta
     4441 ctggacaggc accacccacc tggacaatat gggtgctgtt tatgtttgtt ttggcagggc
     4501 gatgggcttt atggagatgc caccagggtc tggcaactac aggtgtgtgg atgtacacgg
     4561 ggccggtgcc cagcgcagtg acccgaaggc cggcaacccg gctgatttcc caaagggccg
     4621 cggcccgcag ggcgatgttg tcaggataaa caattttgtg aggctggtaa ggagcggaaa
     4681 caagaaaatc aaatagtacc agcacctctt tttgaattct ggtacgctgt ttgcatccat
     4741 gtgtccttcg ggtataatgg caatccatgg gaagggcaga aaaaatcgca agctggacaa
     4801 aagaaaacag gttttttgtt gttgccggtg ttttaattgt actttgcgtg atagttgttg
     4861 ttccaatctt tgcccaatca ttgaatcggc ctgatggcga ttattccaca cctgcgcttt
     4921 acctgttttc cacgatagcc cagacaatgg gcgcaatcct ggggattgtg ttcgcagttt
     4981 tctttgctgt atatgcgaac atgaggtcaa atcccgaaaa tcccacaata gattatgcca
     5041 ggagctacct tcagtctgac cgatatttta tgtccgcagc tcttgtcggc ttgttttgca
     5101 ttctagggtc ggtggtctgc ctactgatga tttatgcttc aggcagttta ttaaacataa
     5161 cagtaatgct ccttcttggc attctttgtt tggttttggg gcttgtgtca atcactttgc
     5221 ttctgtattt tgttacagta agaattcctc tctattttga gccgatcgaa ctttacaagg
     5281 aacaaagaga actccttaaa aagagaagca gtatatcaga aaaagagtat aaaagattcc
     5341 tagttgatac attatttatt tcatctgcag actctaacaa atattatcta agcgaagctt
     5401 tgttgataat aagatgtgat tataaaaatg atgtcagcaa agttagggat tattacaatc
     5461 tattaagaga attaaacatt gcatttactt caagaatatg taaatatcct aaagaaaatc
     5521 agtatagaat aagattgaat attattgaaa cgataaaaga agcgaccaaa tatgacccga
     5581 ataatccaga taaactgaat atgccttata atataagtta ttatcgtggt gattttttta
     5641 tactactaaa gtttcttgtt gataatatcg ctatttgttt tgaagagaat gcggttgata
     5701 aaattaatat aaaaaaaata agtaagatat tttcagaatt tacaggtaat ctatcttttt
     5761 acatgattaa taaacaagaa aaatatattt ttgtattgag tgaattttta aattatttaa
     5821 ggtgtttaca aatagccaat ttcagatttt atgagcttat agatttagaa gcattgttac
     5881 ctatcgttga cgatgttata cgatatttat ttgaaaacaa taatataata aataagatta
     5941 atgataaatt attagaaaat tatattaaat acttatgcat aacatttttt aatataattg
     6001 aatatattga aaaaacagat tacatgattg atttgactaa agatgaatta aaaacaattt
     6061 ttttgtattt agagcaaatc aagaaagggt ttaataatat ttataattgt acagaagcca
     6121 gtctaattaa tcgagaaata agcttagaag aaataatatt taaatactct ttatgttgta
     6181 aattatttga tttcaaatat tataaatata tattattata tatgctaatt aatctacata
     6241 aatactacag taaatcattt gaaaaatctt acacatataa atccatttat gaatcgataa
     6301 atttcccaat tggttatgca atataccttt gtgatcttta tgaaaatggg atagattcca
     6361 aaatagttga tctagattat agaacttatg tagatgtttt tcttaaaaac ttttgtgagc
     6421 agaataatct tgattttagc aagatggaag ctcgatttaa tgacaaattt tacttgaata
     6481 aagaaagatt tgaaaatata caaaaatcat atatgagaat tattttttaa ccccgtcact
     6541 acaaccccgg caaaaatcag cagtccgcca acaagcacgt ttagtcctat cgggtcgcca
     6601 aggaatgccc aggcgatgat cgatgccccg actggctcaa agaggtttgt ggttgtcgtg
     6661 atgatcggcg tcaggtattt gacacccata ttcagcgcgg tgtggccgat cacctgcggg
     6721 aaaagcgcca tgccggcgat ggagagccag gatgctccag aatagcccca gagctgttcg
     6781 cctgtcagga aagcgcctgc aagcaggagc aggcttgcga ctggataaga gacagaaact
     6841 gtctgcagga gaggggccct ttcctgggct atctttgatg tgatcagata gcacgagcca
     6901 aacaatgcgc caagcagtga caagacgacg ccgagcattg ttccttccat ctttgttccc
     6961 cagagtgcaa tcactgccgc accgcctatt gccagaaaaa tgccaaggat tgcaaagggg
     7021 gttggctttt tcctccagat tatatattcg agcattgcga tgaatattgg cgctgtggtc
     7081 acaaggactg ctgttgccga taccgatatg tagtggaagg agcttatcca tacagcaaag
     7141 tgcatcgcta ggaaaaaccc agacagcact gaccagagca gggttttttt gtcatggaac
     7201 attctccacg gcgcgccaaa ggggaccatg attatggagg caatcaggag cctccagaaa
     7261 gccattgaga atggtccggt atctgccgat gcgattttta ccagaggcgc cgcagatgac
     7321 atcgacagca cgc
//
LOCUS       DAOSRI010000052         7303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9511_length_7303_cov_4.632726, whole genome shotgun sequence.
ACCESSION   DAOSRI010000052 DAOSRI010000000
VERSION     DAOSRI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9511_length_7303_cov_4.632726"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..2347
                     /locus_tag="PK717_02510"
     CDS             113..2347
                     /locus_tag="PK717_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46568.1"
                     /translation="MNSSYSDSNGEENNVCLKSAILFGKNVRYNPTLDLFIYSESTNK
                     YLFFNDTNLKKLVGYGGKIEDLRGIAKNIALINGKEIVDLENGQVLFQLSGNNLMSDG
                     AIIVTNDLIYGIKERSIIANISSMNFKPSRIYKNRIESYYLEDGQFGKVSPTIKRIAR
                     GGSLLEIIKTKIAPHDIIKGTYGHFALCFGNATFGKYGNKAYKLVEMSSGAIIFEANF
                     SETRTIQADIIGSKLLLRSEKGAQIYEINRMKEASRINFPIHPQFISDGKDTVVLLPQ
                     LDDGMLGYKLDNGLDIIPWTQTKIPDATEYYLEGDTIYAVTTTTNPYVSNNSPLFYLN
                     STDISCNKLGSQKAASTYSFTLDTPQEGYMASALADSKYYMVRKNGELSVFDCREGSF
                     IQGGNYVGGDFFRAKNPPDGLSGKPSLLISNDRVFLLHPGKTNWLLCFDKGNGNLVFD
                     KRFELGGPYVNKPQIRLFGETLIMESPSGVYFLNDDTVIGGDGLFIGISNGKAIFSDN
                     SKTKFIDLSTKDTGTIEISDIYKSAIPSFGFNNGILTPDGNIRLIDNSWVQKQLGTWF
                     SFKSDTQKNLLSCKNDTTIPGGTLTHFEPCPSFSIKRIKPKSEVDQESVTFEFAMTRT
                     DGLADVWKGEACLVAWGDDGKAPVLAKLDSSKQKIGPLLPGMKQTLTFKFPDKDSSIQ
                     SMPQTKDSSSLKYFALVVESNGLLDTKNSILSDFDKDPRPLFDGTPVALDWQKAIAVT
                     VWGR"
     gene            2348..4852
                     /locus_tag="PK717_02515"
     CDS             2348..4852
                     /locus_tag="PK717_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46569.1"
                     /translation="MKQKLLVIILASSIIFGLSYDHTQSQTNLSVPILPSRMQIYGGE
                     ISVLQPYSTKKLGGLTLNEQLETEAAYPDLGENWIMRRDGSNTFKIVQAYLSNEKPIP
                     TPPNFLESYRLPAMVMKNDILSSIVGPGCVYLPWQNLLLRYNQQGNDKTGNILFYDAT
                     SLEKLGQLENTNLIAVLSNRFVLTSGGIQEIKMGKLSLDIKKIGDQFACDGKLFVSTN
                     VIFDLSKGELVSELNSPNIQKPVRIIKIKKDFIEYYSYSSGVSAITPARARLSKNGGL
                     LEIKQVNIEDSPESWQILDSFNDYALCRNSKSYRIIEMNSGIAVWERGYDESKKPIPE
                     LSFFGDKGLYRTMDFAEIVNLHNFKTEKFLRFPLHPIVLNDSDDFAVILQSSENGDMV
                     GFLLDGNFNIVPTSMTKLPASDKYLLKDNEIVAYNLKTAIDSNSEPTSHPSYLIQGRL
                     SKHKLGSTKPYFEFCIDIPTNSQSLLGSNYIDESLYIATSQWVKKIDINDSRSMVYQT
                     NIEDEKQTCRNLSLFVSEKLIAIQFDYGGSSRLLVFNSKTRELLLDSGMNTNAQEHLI
                     GVFDDIVVTDFRVFLPLQEKELGLDGRFAGMEGRKIVSFWQNNLHTLDVDSGEKTSTT
                     MTEGLYDLDSQYVNGFITNYGRIMSANGSYLQRNLLGMLNDIYKSDKTDPYSLVTKNN
                     GLLMQLSPCPSFSIKQTKPESGANKESVTFEFAMTRTDGLADVWKGEACLVAWGDDGK
                     APVFAKLNEPRHKLGPLLPGMSQEISFKIPEPPLLENNQKGEGRYFALVVESNGLLDT
                     KSSILSDFDKDPRPLFDGTPVALDQQKAIAVTVWGK"
     gene            5063..>7303
                     /locus_tag="PK717_02520"
     CDS             5063..>7303
                     /locus_tag="PK717_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46570.1"
                     /translation="MKNVLIKIIIVIFLVCMPTSGTNSKTDSYPPMLPEKSIPGIEHI
                     EVTDNTLTVYSKTGATEIDKNTGLVAGIKPMAFSFNKYSYPTHYASTSSKPFLRNKSF
                     NMAGDLLTKYEPFVFGNDFVCLIGEEMIGIYKGKVAWKHNKPNRYNRVDELSDSCIVF
                     HGGTGMMVIEKATGIVVLECAYPPASLSFIVGIDSNRTMSFIRDDQVAIIDNCFVVSI
                     EGCPRLLWDLPIKGLPFILKDEVGTIQVSFSKPDQATYFDKLTGEKEKEVVLAYEDMP
                     NPLPLNNYGILGVVQNTAYGYMKTNPLSIFGFDIATGKILFSKPLFCQNQKHEIFDDG
                     KSIFYVSPAGIEKVDSANGKSLWRSPFPDSMRFVNDPDDLYAFWPTYGKDGELENSIS
                     RFGSQEKALSSCPYIDYGFTPNLVPTKYGVVWVPQNGEGEGPVLTGADGTTKTYKLNK
                     WSDYMHPGYACDSETLFVNYNGALVSCIDLKTGNINNIQVSETESTRKDAKKILLVND
                     KWIFTDAGDYYWLAISRIDKSTKKFFKHKQFYSRALINEDCVVLSGTTVFGLRTYFQN
                     TYPGNFLCFCEGGLLVQYNDGIVFIDSESGEFKKIATIKDRVGESLKIGNILVSGEFM
                     ADIDKGEIVQNMLSDLYSSLDEMAIFNNKVVMSENGGYSIFSPCPSFSVKRTGKGGAG
                     EVDEVSFEFRNTREDERDLVLKGEAYLVSWGDDGKAPLFAKLNEPRHKLGPLLPGMSQ
                     EITFKLP"
BASE COUNT         2361 a         1379 c         1562 g         2001 t
ORIGIN      
        1 aaaaaaacct ctacagccct ataataatat tttttacaat ctaggcgatc atctgaattc
       61 gatattcaat attaaacacg attcaaaacc tgaatcagat attatacaaa tcatgaattc
      121 ttcctattct gattctaatg gagaggaaaa caatgtttgt ttgaaatcgg ctattctttt
      181 tggaaaaaat gttcgctata atccaacatt agaccttttt atctattccg aaagtacaaa
      241 caagtactta ttcttcaatg acaccaatct aaaaaaactt gttggatatg gtggaaaaat
      301 agaagatctt cgcgggatag caaaaaatat tgccttaatc aatggaaaag aaattgtaga
      361 tttggaaaat ggccaggtac tttttcaatt aagtggcaac aatttgatgt ccgatggagc
      421 aataattgtc actaatgact tgatctatgg cataaaagaa agaagcatca tcgcaaacat
      481 atcttcaatg aatttcaaac catctcggat ctataaaaac agaatagaaa gttattactt
      541 ggaagatggc caatttggca aggtttcacc aactattaaa agaattgcca ggggtggttc
      601 tcttctcgaa atcattaaaa cgaagatcgc accacacgat ataatcaaag gtacttatgg
      661 ccattttgct ttatgttttg gcaatgcaac attcggtaaa tacggaaata aagcttacaa
      721 actcgtcgag atgtcatctg gagcgataat atttgaagca aatttttcag aaaccagaac
      781 aatccaggct gacatcatag gttccaagct gctattgaga tcagaaaaag gcgcgcaaat
      841 ttatgaaata aatagaatga aggaggcaag cagaatcaat ttccctatac atccacaatt
      901 tatatcagac gggaaagata ctgttgtctt gctacctcaa cttgatgatg gcatgctagg
      961 ttacaaattg gacaatggtc tagatattat accctggact caaacaaaga tcccggatgc
     1021 aacagagtac tatctggaag gtgacacaat atatgcggtt acaactacaa ccaatccata
     1081 tgttagcaat aatagtccac tcttctactt aaactcaacc gatatttcat gcaataagct
     1141 tggctcacaa aaagcggcat ccacttattc attcacactt gatacgcccc aggaaggtta
     1201 tatggcaagt gctctcgcag acagcaagta ttacatggta agaaagaatg gtgaattgag
     1261 cgtattcgat tgtagggaag gaagtttcat tcaaggcggc aattatgttg gcggagattt
     1321 tttcagggcc aaaaatccac ctgatggcct ttcaggaaaa ccatctctgc tgatttccaa
     1381 tgatagagta tttctgcttc atccaggaaa aaccaattgg ctgctttgtt ttgacaaggg
     1441 caatggtaac ctggtttttg acaaacgttt tgaactggga ggcccctacg tcaacaaacc
     1501 acaaattcgc ctctttggtg aaacattaat catggagtca ccatcaggag tgtatttttt
     1561 aaatgatgat acagttatag gtggcgatgg tttatttatc ggcatttcga atggtaaagc
     1621 tatatttagc gataatagca aaaccaaatt tattgattta tcgacaaaag atacaggaac
     1681 aatcgaaata tcggacattt acaaatctgc aatcccatct tttgggttca acaatggtat
     1741 tcttacacca gatgggaata taagattaat cgataattca tgggtacaaa aacagctggg
     1801 aacatggttt tcattcaaga gtgatacaca aaaaaatctg ctatcttgta aaaatgacac
     1861 gacgatccct gggggtacct taactcattt tgaaccttgt ccctcatttt caatcaaacg
     1921 aatcaaacca aaatctgagg ttgaccagga gagtgttact tttgagtttg ctatgacgag
     1981 aacagatggt ctcgcagatg tctggaaggg tgaggcatgt ttggttgcct ggggggatga
     2041 tgggaaagca cctgtgttgg caaaattgga ctcatcaaag caaaagatag gaccattatt
     2101 gcctggaatg aaacagacat tgacattcaa gtttcctgac aaggattcaa gtatacagag
     2161 catgccacaa acaaaagatt cctctagttt gaagtatttt gctcttgttg ttgagtccaa
     2221 tgggctacta gacacaaaaa actccattct ctcagatttt gataaagacc ctaggcctct
     2281 ttttgatggt actcctgttg ctcttgactg gcagaaggcc attgccgtga ctgtgtgggg
     2341 gaggtagatg aagcaaaagc tgctcgttat tatattggca tcatcaataa ttttcggcct
     2401 atcatatgat cacacacaaa gccaaaccaa tctgtctgta ccaatattgc catcaagaat
     2461 gcaaatatac ggcggtgaaa taagcgttct tcagccatac agcacaaaaa aactgggcgg
     2521 attgactcta aatgaacaac tagaaaccga ggccgcttat ccagatcttg gtgaaaactg
     2581 gataatgcgg cgggatggat caaacacatt taaaattgtg caagcatatt tatcaaatga
     2641 gaagcctata cctacgccac ccaacttcct ggaaagttat agattgccag caatggtaat
     2701 gaaaaatgac atcttgtctt caattgtggg gccagggtgt gtttatttgc catggcaaaa
     2761 cctactacta cgatacaacc agcaaggcaa cgataaaact ggcaacatat tattttacga
     2821 tgccacctcg cttgaaaaac tgggccagtt agagaatacg aatttgattg cagtcttatc
     2881 aaatcgattt gtactaacct caggcggaat ccaggaaatt aaaatgggaa aattgagcct
     2941 ggatataaaa aagattggcg accaattcgc atgtgacggt aaattatttg tgtcgacaaa
     3001 tgtaattttt gacttgagta aaggagagct cgtatccgaa ctcaatagcc cgaacataca
     3061 aaaaccagta aggattatca aaataaaaaa ggatttcata gaatattact cgtattcttc
     3121 cggggtcagc gcaatcacac cagcaagagc aagattgtca aaaaacggtg gtttactaga
     3181 gataaaacaa gtaaacatag aggatagtcc agaaagctgg caaatactgg attcattcaa
     3241 tgactatgct ctttgcagaa atagcaaatc atacaggatc atcgagatga attctggcat
     3301 cgcagtctgg gagagagggt atgacgagag caagaaacca ataccagagc tgagcttttt
     3361 tggtgataaa ggactgtata gaacaatgga ttttgcagag atagtaaacc tgcacaattt
     3421 caaaacagag aagtttttaa ggttccccct gcatccaatt gtcttgaatg attcggatga
     3481 ttttgctgtc attttgcaat catctgaaaa cggcgatatg gttggattct tgctggatgg
     3541 caactttaat attgtgccaa cgtcaatgac caaacttcct gcgtcagata aatacctgct
     3601 caaagacaac gaaattgttg cttacaacct aaaaactgcc atcgatagca acagcgaacc
     3661 aacaagccac ccttcatatt taattcaggg tagattgtca aaacataaac ttggttccac
     3721 caaaccttat ttcgaatttt gcatcgatat tccaacaaat tcacaatcgc ttcttggttc
     3781 aaactatatt gatgaatcat tgtacatcgc tacatcgcaa tgggtaaaaa agattgatat
     3841 taacgatagt agatcaatgg tataccaaac aaatattgaa gatgaaaagc aaacatgtag
     3901 aaacttgtct ctgtttgttt ctgaaaagct aattgccatt cagtttgatt atggtgggag
     3961 cagtcgtttg ttagtgttca atagcaagac tagagagctg ctccttgatt cagggatgaa
     4021 cacaaacgct caggaacatc tcattggggt ttttgatgat attgtcgtaa cagatttcag
     4081 ggttttcttg cctttgcaag aaaaagaact tggccttgat gggcgatttg ccggaatgga
     4141 gggtagaaaa attgtaagtt tttggcaaaa taatttgcat accctcgatg tagacagcgg
     4201 cgagaaaact agcaccacaa tgacagaagg tttgtatgat ctagattcgc agtacgtgaa
     4261 tggtttcatc accaattatg gtcgaataat gagcgccaac ggttcctatc ttcaaaggaa
     4321 cctccttgga atgctaaatg acatttacaa gtcagataaa actgatccat actcacttgt
     4381 gacaaaaaat aatggtttat taatgcaact ttccccttgc ccctcatttt caatcaaaca
     4441 aaccaagcca gaatctggag ctaataaaga gagcgttact tttgagttcg ctatgacaag
     4501 gacagatggt ctcgcagatg tctggaaggg tgaggcatgt ttggttgcct ggggagatga
     4561 cgggaaagca ccagtgtttg ccaagttgaa tgaaccaagg cacaagttag gacctcttct
     4621 tccagggatg tcacaggaga ttagctttaa gattcctgaa ccacctttac ttgagaacaa
     4681 tcagaaaggt gaagggaggt attttgctct tgttgttgag tctaatggtc tgttagacac
     4741 aaaaagctcc attctttcag attttgataa agaccctaga cctctttttg atgggactcc
     4801 tgttgctctt gaccagcaga aggccattgc cgtgactgtg tggggaaaat aagttgtgct
     4861 cactgtgagc acagggggtt tcaatcgcta ctgcatacct cgaataataa gggggggtgc
     4921 taccggtttt tccaaaatgt tgctggattg aaaaacacca gatcagcttt atgaataata
     4981 aaccggtggg ggccttggct tggtattatg aaatgaagcg acaagaaagt ttttatgtct
     5041 gggaagaggg gaagagcggg caatgaaaaa tgttttgata aaaatcatta tcgtgatttt
     5101 tctggtttgc atgccaacaa gtggcacaaa ctctaaaaca gattcctacc ctcccatgct
     5161 cccggagaaa agcattccag gaatagaaca catagaggtc acagacaaca cactgactgt
     5221 ctactccaaa accggtgcaa ccgagataga caaaaatact ggccttgtag ctggcataaa
     5281 accgatggct ttttccttca acaaatactc ttatcctaca cattacgcgt caacatcatc
     5341 aaaaccgttt ctccgcaaca agagtttcaa tatggcgggc gatttactca ccaaatatga
     5401 gccatttgtt tttgggaacg attttgtctg tctgattggg gaggaaatga ttggtattta
     5461 caaaggcaag gtcgcctgga agcataataa acccaaccgg tacaatagag tagacgaatt
     5521 gtctgattct tgcatagttt ttcatggcgg aacaggcatg atggtgattg aaaaggcaac
     5581 aggaatagtt gtcctggaat gtgcttatcc gccagcttcc ctttctttta ttgtgggcat
     5641 agactccaat agaacaatgt ctttcataag agatgaccag gtggccataa tagacaattg
     5701 ttttgtggtg tctattgaag ggtgcccaag attattgtgg gatttgccaa taaaggggct
     5761 tccatttatc cttaaggatg aggtaggaac aatacaggta tctttctcta agcccgatca
     5821 agcaacatat tttgacaaac ttactggtga aaaagagaag gaagttgttc ttgcctatga
     5881 agatatgcca aatccattgc cgctaaataa ctatggtatt ttaggtgttg tgcaaaacac
     5941 cgcctatggg tatatgaaga caaacccgct ctcaattttt ggattcgaca ttgcgacagg
     6001 aaaaatactg ttctcaaaac cactattctg ccaaaatcaa aaacatgaaa tatttgatga
     6061 cggaaaatcc atcttttatg tttccccagc cgggatagaa aaagttgact ctgcaaatgg
     6121 gaaatcactc tggaggagtc cgtttccaga cagcatgcgg tttgtgaatg acccagatga
     6181 tctttacgct ttctggccaa cctatggcaa agatggtgaa ctggaaaatt caatctcccg
     6241 ttttggctcg caagaaaaag cactctcgag ttgcccatac atcgattatg gtttcacccc
     6301 aaatcttgtc ccaacaaaat acggcgttgt ttgggtcccg caaaatggtg aaggcgaggg
     6361 gccagtactg acaggtgcag atgggacaac caaaacctac aaactaaaca aatggagcga
     6421 ttacatgcat cctggctatg cctgtgacag tgaaactctt tttgtaaatt acaatggagc
     6481 tttggtatcc tgtattgact tgaaaactgg caatatcaac aacatacagg taagcgagac
     6541 tgaatccaca agaaaagatg cgaagaagat attgctggta aatgacaagt ggattttcac
     6601 cgatgccggg gactattatt ggctggcaat tagcagaata gacaagagca ccaaaaaatt
     6661 tttcaaacac aaacaatttt actctagggc tctaataaat gaagattgcg ttgtgctttc
     6721 aggaacaacc gtttttggcc tgagaacata tttccagaac acatatccgg gcaattttct
     6781 ttgtttttgc gaaggaggcc tgcttgtcca atacaatgac gggattgtat ttattgactc
     6841 cgagagcggg gaattcaaaa agatagcaac aataaaggac cgtgtgggtg agtcgctcaa
     6901 aatcgggaac attcttgttt ctggggaatt tatggcggac attgataaag gggaaattgt
     6961 ccagaacatg ctatcagacc tgtattcaag cctggatgaa atggcgatct tcaacaataa
     7021 agtggtcatg tcagagaatg gtggttactc aatattctcc ccatgcccat cattctcggt
     7081 caaaaggaca gggaagggtg gagcaggtga ggtggatgag gttagctttg agttcaggaa
     7141 tacaagggag gatgaaagag accttgtgct aaagggagag gcgtatctgg tttcttgggg
     7201 ggatgatgga aaggcacctc tatttgccaa gctgaatgaa cctaggcata agttaggacc
     7261 tttgcttcct gggatgtcac aggagattac attcaagctt cct
//
LOCUS       DAOSRI010000053         7256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9579_length_7256_cov_3.165949, whole genome shotgun sequence.
ACCESSION   DAOSRI010000053 DAOSRI010000000
VERSION     DAOSRI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9579_length_7256_cov_3.165949"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PK717_02525"
     CDS             complement(<1..1560)
                     /locus_tag="PK717_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46571.1"
                     /translation="MRKLLATMLTLAVALTVVPFFGEIPQAYAETKNILFAVVRWSPL
                     GQDRSGQPLSGRVVPWTSGDISTWWGRQQFPMPSQPRYWSEVYLTVIGAGGQSTEKDK
                     MNCILDSNGNIWFDPDGYFHNAAERARPDPPGQCYDYNFKTDNTPANNTQGPYRVGDY
                     ANFFHNKGYWFTPISHQHGPYTFPRNFSGRLFKLGQAALPGNGMALVMSETFLASCDP
                     NTSYNLSVETDMWEGVNPAVTAASIFDPENPTLRRSQRIQKSTVLGPTGLDFQVPATT
                     FFNVKPSYRSYVGIEIFRDSGENNINNPQGQCFAQNLSDDYKALYACEDFIGASNSGV
                     NDSDTGLPLKPFPASFRYYDSGGTGFGCGEPIYDNLNTAQDPFMNDIVNPGDKRMTTV
                     TFSVGGAIVTYEAGSYVSAGDVDNGYIIRPFTNVGWFDANNNNEFEEGEWIYQDNDLS
                     NSVTAGDVRLTVVNKNGNTYQCGTVVAGPELYIRSYPISGITVGRCGNFRFMDMEVLP
                     GVAPVQVNVSRP"
     gene            complement(1745..3061)
                     /locus_tag="PK717_02530"
     CDS             complement(1745..3061)
                     /locus_tag="PK717_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOR46572.1"
                     /translation="MLFEEEYNYTPIDKTEEPETIDTEYKEPAHEEKPQYSQMNINGG
                     RQADRQNTPRWGGIIALALVFSVLFGGLSGFLGYRLADNKNSTVVIQQPVPETPINQQ
                     MFYDGVVDVVNKVSPAVVSITVDKEITNSILQFDNPFEQFFNNDNNVNPFARQQPQVR
                     VEQAAGSGVIIKNTWTDEQGFILTNNHVVENGQKIVVHLIDKRNFEAQIVATDSLSDL
                     AILKVKEKNLPMAELGDSSKLKVGQPVVAIGNPYKFENTVTTGVISALARNIQVDKSR
                     MLVGVVQTDAAINPGNSGGPLVTLDGKVVGINTVIIEGAQNLGFAVSSNVASRVAKDL
                     IKFGKVGWPVLGVRGATLTQDLSSQLGLKYASGAYIAVVEKGGAQEAGIQRGDIITAI
                     DGKEVTDFEGLLVEVRNHQVGDVVKIRVSRDGREMTFNVKLGAAAE"
     gene            complement(3075..4184)
                     /locus_tag="PK717_02535"
     CDS             complement(3075..4184)
                     /locus_tag="PK717_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOR46573.1"
                     /translation="MENSLVFALYLVVAWPFLWFLLGRKLSPKGLGWSIFVSLASLFG
                     FSAGFIMLSSLVPETVKLTLTPTKLFSTEGIIYGANVTLEPTFWHYLLIAAPWIVIGF
                     VTFRLGFGLFLLSSRTRGYRVAPKSFRESLSRGFGGQDTRSTTFFLGNSGSEAFSTLN
                     SVYISENLIKGLSKEQLDAVVAHEMAHIRRRDIINMWLWTLNVALCSYLPGKFAWKSY
                     SLELEKETDRLAIKAIGSPFPLAEALIGVAKLCSPVRVAANLSSGGLEERTMALLEPK
                     KEKLVAHKSLATVLCFTLLLLMPLWPEKTNSLVEMGISEVQAQKMIEGKMVALVKPTG
                     DAGKPYQVNFYPEQAIKIDGFGNLILDKRLVIRKL"
     gene            complement(4174..4590)
                     /locus_tag="PK717_02540"
     CDS             complement(4174..4590)
                     /locus_tag="PK717_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOR46574.1"
                     /translation="MEDKVIRVTSNRVGAGLVLGSLEYEVYKACCAIGKATVREIVDH
                     LSTDHAYTTIMTTCDRLSKKGVLKREKCGKSYVYMPSVTQEEMDVKSTCKVLNSVIGS
                     LTEPVMANFLEMLENSDSNKLDMLERMIKERKQNGK"
     gene            complement(4730..6064)
                     /locus_tag="PK717_02545"
     CDS             complement(4730..6064)
                     /locus_tag="PK717_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46575.1"
                     /translation="MKKMARVFYAIGLILVFLVGCGASNEGIGQITPQGEKRFYTLGY
                     GDDWETQMRKREIIIYSAQLDWSDFTSAFKVKLPRIRDPYMEGLLKYTVATNFGSAFY
                     FYLEGPHTCSLEGLSPDKQWALLSAGTGFYFEIPHKEDGKLLKEISAKYTILYNLKNG
                     SSKKLGARYRKSFYHDDLGELVKESGVLYFPVYWGEKELLLVEQTKENKFFGTSGKYC
                     DPRVVTMDLTHAQVPYAHARGVRLSLDDLSITTTDRVKPIRPWVTFADQTTGIVGYVG
                     PYDKTYPESSDIPNITNVNCERNTYAIADMSGKKIWIFQEKLKNFEDSEYKTCEYAIV
                     STSKDSVFIFAGYMKPGFGEAVAPRYRNYLLRLDMNSGEVKKIKEFDQNTFSDGCFIR
                     ASHPDKAYIFAGIHNEAKTKVERVLMEYLDGKLFKVQPQNEIPRIGEDYYIR"
     gene            complement(6209..6658)
                     /locus_tag="PK717_02550"
     CDS             complement(6209..6658)
                     /locus_tag="PK717_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46576.1"
                     /translation="MHAVDALLVGSVMLDRQRMLEAALKTWRFVADCQLQDGGFPMLV
                     GEGENYERADVCAHFIRAGRLIEALGMLPEGYCLKIDKATKRLCGELANRQPDPKMFG
                     AFNHSGAERPDLQDANTQATMVAIQALSLEAGMPNAASAIARDMLIV"
     gene            complement(6622..>7256)
                     /locus_tag="PK717_02555"
     CDS             complement(6622..>7256)
                     /locus_tag="PK717_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46577.1"
                     /translation="YSTLEHCHSIMFSTTTGNAITCMLFLDRMLPGWPRQNPTEQAAF
                     WLLDKAWVADEGGFMVGINPQNDSQIRQIKTLDVAMILNAFVHLHYETDNSFCLSVAS
                     KAVDFITQRMMGPNGVPYPLLDVDSATVIQDTSNWRADIQPYLAKIAIGLMNYAQAIR
                     SERARTHAIRLLDWACQSAEEKQEGAITSTEDRSICFQQCMLLTHSLLVR"
BASE COUNT         1819 a         1805 c         1640 g         1992 t
ORIGIN      
        1 cggtctcgag acattcacct gcacaggtgc aacacccggc aggacctcca tgtccatgaa
       61 gcggaaattg ccgcaccttc caacagttat accagaaatc ggatagctcc ttatgtaaag
      121 ctcagggccg gcaacaaccg ttccgcactg ataggtgttg ccattcttgt tgaccaccgt
      181 cagacgaacg tcaccggcag ttacggagtt ggaaagatca ttgtcctgat aaatccattc
      241 accctcttca aactcgttgt tattgttggc atcaaaccag ccaacgttcg tgaatggacg
      301 aataatatag ccattatcga catcaccggc tgaaacatac gagccagcct cataggtgac
      361 tatagctcca ccaacactaa acgtgacggt ggtcatacgc ttgtcgccag ggttgacgat
      421 gtcgttcatg aacgggtcct gggcggtgtt gaggttgtca tatatcggct cgccacagcc
      481 aaaaccggtt ccgccgctgt cgtaatacct gaagcttgcc gggaagggtt ttagtgggag
      541 gccggtatcg gaatcattca ctcctgaatt tgaggcgcca atgaaatcct cacaggcata
      601 caaggccttg tagtcatcag aaaggttttg ggcaaagcac tgaccttgcg ggttgttgat
      661 attgttttca ccagaatccc taaatatctc aatgcctacg tagctcctgt aagacggctt
      721 aacattgaaa aaagtggttg ccgggacctg aaaatccagg ccagtagggc caagcacggt
      781 tgatttctgg attctctgcg atcttctgag tgtcgggttt tcagggtcaa agattgatgc
      841 agcggtaacg gccggattga cgccttccca catatcggtt tcaaccgaga ggttgtagga
      901 ggtgttcgga tcgcaagatg caaggaaggt ctctgacatg acaagagcca ttccattgcc
      961 aggaagcgca gcctgcccca acttgaaaag cctccctgaa aaattgcgcg ggaaggtgta
     1021 tgggccatgt tgatggctga tcggcgtgaa ccagtagccc ttgttgtgga agaagtttgc
     1081 atagtcgccg accctatatg ggccctgtgt gttgttggcc ggagtattgt ctgtcttgaa
     1141 gttgtagtcg taacactgac ctggcgggtc tggtcttgct ctttcggcag cattgtggaa
     1201 atagccgtcc gggtcaaacc agatgttgcc gtttgagtcc aggatgcagt tcatcttgtc
     1261 tttttcagtg gactgcccgc ctgcaccaat aacggtaaga taaacttccg accaatacct
     1321 tggctgggac ggcatcggaa attgctgcct gccccaccag gtggaaatat cgccggaagt
     1381 ccacggaacg accctgccgg agagtggctg gccgctcctg tcctgtccaa gcggagacca
     1441 cctgactact gcaaaaagta tgttttttgt ttcggcataa gcctgtggta tttcgccaaa
     1501 aaacggaacc acggtgagcg ccaccgcgag cgttagcatt gtagccaaga gctttctcat
     1561 cttgaaactc ctttcgatgc attcatggct gatttgcttc aaatacacga aatcttgggg
     1621 tcaagttcaa aattggggag ggtgctgatg tcttgataaa aaatagaatc tttttttgaa
     1681 gacaaacaaa aaaggcgggg gcaaccccgc cttttttgtt ccagaaggta cttattggtg
     1741 atggctattc cgcagcagca ccaagtttaa cgttaaaggt catctctctt ccatccctgc
     1801 tgaccctgat cttgacgaca tctcccactt gatggttcct tacctcaaca aggagtcctt
     1861 caaaatctgt aacttccttt ccatctattg cggtgatgat atcacccctc tgtattcctg
     1921 cttcctgggc accacccttt tcaaccactg ctatgtatgc acctgacgca tacttcaaac
     1981 caagctgcga agagaggtcc tgtgtgagtg ttgcgcccct gacaccaagc actggccagc
     2041 ccaccttgcc gaacttgatg aggtctttgg caactcttga cgcaacattt gaggagactg
     2101 caaaacctag attttgtgca ccctcaataa tgactgtatt gattccgacc accttgccat
     2161 ccagtgtcac aagtggccca ccggaattgc ctgggttgat ggcggcatcc gtctgaacaa
     2221 ctccgaccag catacggctt ttatctacct gtatgtttct ggcaagggcc gagataacgc
     2281 ccgttgtgac tgtattttca aacttgtatg ggttgcctat tgcaacgacc ggctgaccaa
     2341 ctttcaattt tgaggagtcc ccgagctcgg ccattggaag atttttctct ttcaccttga
     2401 ggatagcaag atcggaaagt gaatctgtgg cgacaatctg ggcctcaaaa ttcctcttgt
     2461 ctatgaggtg gacaacgatc ttttggccat tctccacaac atggttgttg gtgaggataa
     2521 aaccttgttc atcagtccag gtatttttta ttatgacacc agaacctgcc gcctgctcga
     2581 cccttacttg aggctgctgt ctggcaaatg ggttgacgtt attgtcgttg ttgaagaact
     2641 gctcaaaagg attgtcaaac tgcaatatgg agtttgtaat ttccttgtca acagttatcg
     2701 agacaacagc aggagatact ttatttacca catcaacaac accgtcgtag aacatctgct
     2761 gatttatcgg ggtttccggc accggctgct ggatcaccac ggtactattt ttgttgtctg
     2821 caagcctgta tcccaggaac cctgagaggc caccaaaaag caccgagaat acaagagcaa
     2881 gggcaataat tccgccccat ctcggggtgt tctgcctgtc tgcctgtctg ccaccattta
     2941 tattcatctg cgaatactgc ggtttctcct catgagctgg ttccttgtac tcagtatcaa
     3001 tagtttccgg ctcttcagtt ttatctatcg gggtatagtt atactcttcc tcaaataaca
     3061 tctattcttc ctccttatag ctttcttatg acaagcctct tgtccagtat caggtttcca
     3121 aaaccgtcta tctttatggc ttgttcaggg taaaagttga cctggtaagg ctttccggca
     3181 tcaccagttg gcttgacaag ggccaccatc tttccttcaa tcattttttg cgcctgcacc
     3241 tctgaaatcc ccatctctac aagggaattt gttttttccg gccagagggg catcaaaagc
     3301 aacagtgtga agcagagcac tgtagcaaga gatttgtgcg ccacaagttt ttctttcttt
     3361 ggttcgagca aagccatggt tctctcctcg agtccgccag aagaaagatt tgcggccact
     3421 cttaccggtg aacagagctt tgcaacacct ataagtgctt ccgcaagcgg gaagggtgaa
     3481 ccaatcgcct ttattgcaag gcggtcagtc tccttttcaa gctccagcga atagctcttc
     3541 caggcaaact ttcctggaag atacgaacaa agtgcaacat tgagcgtcca gagccacatg
     3601 ttgattatgt ctctgcgcct tatgtgggcc atctcatggg caacaactgc atcaagttgc
     3661 tcttttgaaa gcccctttat caaattttct gaaatgtaaa cagagtttag ggtcgagaag
     3721 gcctcggaac ctgaattgcc aagaaaaaaa gttgtgctcc tggtgtcttg cccgccaaat
     3781 ccacgagaaa gcgattccct gaaagatttt ggtgcgacac ggtaccctct ggtccttgag
     3841 gacagcagaa acagcccaaa cccaaggcgg aaagtaacaa aaccgattac gatccacggc
     3901 gcagcgatga gaagataatg ccagaaggtc ggctccagcg tgacattggc tccgtaaata
     3961 ataccctctg tggaaaagag ctttgttggt gtgagggtga gttttactgt ctccggaaca
     4021 agactggaga gcattatgaa gccggcagaa aacccaaaga gggacgccag tgacacaaaa
     4081 attgaccagc ccagcccctt cggggagagc ttcctgccaa gcaggaacca caagaacggc
     4141 cacgccacaa ccagataaag ggcaaaaaca agactatttt ccattttgtt tcctctcctt
     4201 tatcatacgc tcaagcatgt ccaatttgtt cgagtccgaa ttctcaagca tttccagaaa
     4261 atttgccatg acaggctcgg taaggctgcc aatgaccgag ttaagtacct tgcacgtgct
     4321 tttaacgtcc atctcttctt gggtgactga cggcatgtaa acatagctct tgccgcattt
     4381 ttcccttttg aggacaccct ttttggagag cctgtcgcag gttgtcatga tggtcgtgta
     4441 ggcatgatcg gtcgaaaggt ggtctactat ttcccttaca gttgcctttc cgatggcgca
     4501 gcaggccttg tagacctcgt actccaggga gccgagaacc agccctgcac cgaccctgtt
     4561 tgacgttacc cttataactt tgtcttccat gatggtgggt attttacaat caatagtact
     4621 acagtcaata gtataagatt aaggaatggt ttcgtgtaaa aaaatgtaga aaatagctga
     4681 cttatagccg attaaagaga attgggtctg tccattccga acatcctaat tacctgatat
     4741 agtaatcctc tccaatcctc ggtatttcat tttggggttg caccttgaag agcttgccgt
     4801 ccaggtattc catcaatact ctttcgactt ttgtttttgc ttcattatgg atacccgcaa
     4861 aaatataggc tttgtctgga tgagaagctc ttatgaaaca gccatcagaa aacgtatttt
     4921 gatcaaactc ttttattttt ttcacttcac cactgttcat gtccagcctg agcaaataat
     4981 tcctgtatct tggtgcaact gcctccccaa aacctggttt catgtaaccg gcaaagataa
     5041 aaacagaatc tttcgaagtt gaaactattg catattcaca ggttttatat tcactatctt
     5101 caaaattttt caacttttcc tggaatatcc atattttttt accactcatg tcagcaatag
     5161 catatgtatt gcgttcacaa tttacatttg tgatgtttgg tatatcacta ctttccgggt
     5221 atgttttgtc atagggccca acatagccaa caatgccggt ggtttgatcg gcaaacgtga
     5281 cccatggtct aatgggtttc actctatccg ttgtggtaat gcttagatcg tccagggaaa
     5341 gtctgactcc acgggcatgg gcataaggca cctgtgcatg agtaaggtcc attgtcacaa
     5401 cccttgggtc gcaatatttt ccactggtgc caaagaattt gttttctttt gtctgttcaa
     5461 caagcaacaa ttctttttcc ccccaataaa caggaaagta aagaacacca ctttctttta
     5521 ctagctcccc aagatcatca tggtaaaaag attttctata tctggcacca agtttttttg
     5581 atgaaccgtt tttcaggtta tacaaaatgg tatatttggc cgatatttct tttagcaatt
     5641 taccgtcttc tttgtgtggt atttcaaagt aaaacccggt ccctgcagaa aggagggccc
     5701 attgtttgtc aggtgaaagc ccctccaggc tacatgtgtg cggaccctcc agatagaaat
     5761 aaaatgcact gccaaaattt gtcgccacag tgtattttag caggccctcc atgtaagggt
     5821 ccctgattct tggcaacttt accttaaatg ccgacgtgaa atcactccag tcaagctggg
     5881 cggaatagat gataatctct ctttttctca tctgggtttc ccagtcatcg ccatagccaa
     5941 gggtataaaa acgtttctct ccctgtggcg taatctgccc tatcccctcg tttgaggccc
     6001 cgcaaccgac aaggaacacc aaaatcagac ctattgcata aaaaactctg gccattttct
     6061 tcattaagca acccccagaa aaacttttta tctgctcaga accagtcaag aattatagca
     6121 gagaagaagg ggtttttgta tatttttgta atgatgttgt tcgagagtgt tgaaaaaatc
     6181 tatatatttc ggggttatcg actaaacatc acacaatcag catatctctg gcaatcgcag
     6241 aggccgcatt cggcatgcct gcctcaagcg atagcgcctg aatggcaacc atcgtggcct
     6301 gggtgttggc gtcctgcaaa tcagggcgtt ctgcaccaga atggttgaat gctccaaaca
     6361 tctttgggtc tggttgcctg ttggcaagct ccccgcaaag cctttttgtt gccttgtcta
     6421 ttttcaggca gtagccctcc ggaagcatcc ccagggcctc gatgagcctt ccggccctga
     6481 taaaatgggc acacacatcg gccctttcgt agttctcgcc ctcgcccacg agcatcggga
     6541 aaccaccatc ctggagctgg cagtctgcaa caaaacgcca tgtcttcagt gcagcttcaa
     6601 gcatcctctg cctgtccagc atcaccgaac caacaaggag tgcgtcaaca gcatgcattg
     6661 ttggaagcag atggaccgat cttcagttga ggttattgcc ccttcctgct tttcctctgc
     6721 gctctgacac gcccagtcaa gcaaccttat tgcatgcgtt ctggcccttt cagaccttat
     6781 tgcctgcgcg tagttcatga gccctatggc aatttttgct aggtacggct ggatgtcggc
     6841 gcgccagttc gatgtgtcct gtatgactgt tgcactgtca acatcaagga gaggataagg
     6901 aacgccgttt ggccccatca tgcgctgggt tataaaatca actgcctttg aggccacaga
     6961 aaggcaaaaa gagttgtcag tttcataatg cagatgcaca aaagcattca ggatcattgc
     7021 cacatcaagc gtttttattt gcctgatctg ggagtcattt tggggattga tgcccaccat
     7081 aaacccaccc tcatcagcca cccatgcctt gtcaagcaac caaaaggccg cctgttctgt
     7141 cgggttttgc ctcggccagc ccggcaacat cctgtccaga aaaagcatgc aggtgattgc
     7201 atttccagtg gtggtagaaa acatgatgga atggcagtgc tcaagtgttg aatacc
//
LOCUS       DAOSRI010000054         7252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9586_length_7252_cov_3.692233, whole genome shotgun sequence.
ACCESSION   DAOSRI010000054 DAOSRI010000000
VERSION     DAOSRI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_7252_cov_3.692233"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1329
                     /locus_tag="PK717_02560"
     CDS             133..1329
                     /locus_tag="PK717_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46578.1"
                     /translation="MGRILAFLLSVIVPCSSFGVATSKYIKNESVIEDLCKSMIVSSS
                     FDGKELYFVPTKDNCKLTKRDVYCFNLDAGELENITNTSDKLEYEVMVGEKYIAWFES
                     SRSDPDRVVFMNRTTKNKKYIVRDSLRIRNVSYALSGEYFLYRKFVSKDNMVSHELWA
                     YNIEKESEMQISQTVPYTHPIEYISDGRMVFYSTTVNKKNGSDIFSFNLETLEQKSVC
                     TEVGAQKNLDLKGDILVWEDHRKGSWDSDIYGYRISEGLVFTIDTSRYCTYAPKISGN
                     CVVYRAVYESPPDRKSTSYIYLADTSSNPITPIKLSSSETPKDNLMIDGDFLLWVDYK
                     RKNRPILEEYTIWGYCVSDRIEFEAISGKTSNNWLVDFKKGIFLWSDSEDLNYFDKIH
                     INTIGR"
     gene            complement(1331..3322)
                     /gene="feoB"
                     /locus_tag="PK717_02565"
     CDS             complement(1331..3322)
                     /gene="feoB"
                     /locus_tag="PK717_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOR46579.1"
                     /translation="MANNDLTIALAGNPNTGKTSVFNALTGSTQAVGNWPGVTVEKKT
                     GRMLVDGVQAEIVDLPGTYSLSAFTEDEIVARDFLVKNTPDVAINVVDSTALEHSLFL
                     TLQLIELEVPLVIALNMQDEAQKKGIKIDSAKLSRILGVPVVETVGSTGRGMAELKKT
                     AIQVGKSKQKSHYRVDFGTDIEVSAKKIECAISSDEVSKTSYKTHFFAHKLLEKDPQV
                     EHELDSIAHSEKVHEIVAMEIKKLTKIYGDDIETLVADRRYAAANGLAKMVTISSPQG
                     KAEVTDAIDSVLINKFFGIIFFLVILWGVFQLTFLLGNPIGELIGTGLDKLGAWIQQT
                     LQSVPWLGGVLHDAIINGVGGVLALFPNIFILFVFLSALEDSGYMARIAFVMDGLMSR
                     IGLSGKAFVPFVIGFGCSVPAVMATRTLESKRDRIITILSAPLVTCSARMEVLVFVAS
                     AFFGASAGNFVWAVVALSMTLSVGTAKFFGLVLFKNQPPTPLLIELPPYRVPTVRSVL
                     IHTWDRAKHFIKKAGGVILIGSLVFWVASNIPWGVRLENTIIGQIGHFISFAFTPLGL
                     DWRASVALLFGIVAKEVVIAVFGQLGDGSHDNISKWYSSSSALGLMVFLNLYTPCLAT
                     IAATKAEAGWKWATFQVAYSLALAWIMAFLVVKVSVLFL"
     gene            complement(3335..>4696)
                     /locus_tag="PK717_02570"
     CDS             complement(3335..>4696)
                     /locus_tag="PK717_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR46580.1"
                     /translation="NGEGALTIEVTNISSDNTISRMIRLVEEAQERKAPAERFVDRFA
                     KYYTPVVIVLAILVASVPPIFFGAPFLNLANGTQGWLYRALELLVVACPCSLVISVPV
                     TIVSAISNAARNGVLIKGGAYLEELSRTKIIAFDKTGTLTEGKPRVIKVKSENCLSCE
                     GGDCKHCNDLLAIASSVESRSEHPLAQAILEEARARGIHAHYKPAENVMALSGKGVSG
                     KVNGDSVFVGSHSFFDSNVPHDPLTCEEINLATIQGYTPLLVSIEGKYSGFITVADTV
                     RDTSKVAVDKLKKMGIENLVMLTGDAKATAKLIADQVGIEKVYSELLPEKKLDAIKDL
                     MGSEKHVAMVGDGINDAPALAESSVGIAMGSTGTGQAMETSDVVLMSGDLSKLPFTFR
                     ISRHAMRTIYQNIAFSLAVKASFFVVVLLGFGTMWLAVLADVGVTFLVSLNGMRLLRF
                     RDR"
     assembly_gap    4697..4706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4707..5589)
                     /locus_tag="PK717_02575"
     CDS             complement(<4707..5589)
                     /locus_tag="PK717_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOR46581.1"
                     /translation="MEFRLSGLSCPDCAKGLEKTVSRIDGVDSCRLVFSASKIIVEGT
                     MPHEVVAKTIKDMGYEIVEEDKTSNTPKKNKRYKGFIGFIQFLLGDRDTILALIGFVL
                     VFPGLVFDELLPFLGIGTPVFEYMAIAALIIAGYPVFISAFKNLFSNHEITMNLLMVI
                     AGVGGVVIGAYTEAGLVMVLFMIGEALEGYTMEQSRDAIRKLMEVAPNDALVLRPCVD
                     CEEHMGKDGYNGGPCPFCGPEEKKVPVEELVVGDRIVVKPGEKIPMDGIVISGSSSVD
                     QAPITGESIPVYKCEEDR"
     gene            complement(5731..6018)
                     /locus_tag="PK717_02580"
     CDS             complement(5731..6018)
                     /locus_tag="PK717_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOR46582.1"
                     /translation="MEIETSLPRPLTSFRPGDEIEIVSIPGCSMRKRLADLGITAGAC
                     IKVLRSDFSGPVLVLVKCVRLAIGRGMASKIICRETSKCLLQQQKDENCPI"
     gene            6461..>7252
                     /locus_tag="PK717_02585"
     rRNA            6461..>7252
                     /locus_tag="PK717_02585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1923 a         1661 c         1667 g         1991 t
ORIGIN      
        1 acaggatgta tttcttgctt ggtgtaaagc ggtaatttgt aggcaataaa tatataattc
       61 acatttttct tttgcggagg gattgttttt aatttctgac agtgtaaaaa atacttcaag
      121 gaggtaatta ttatggggcg tatcttggcg tttttgctgt cagtcatcgt gccttgctct
      181 tcctttgggg ttgccacgag caaatacata aaaaatgagt ctgtaattga agacttgtgc
      241 aaaagcatga tcgttagttc atcatttgat ggcaaagagt tatactttgt gcctacaaaa
      301 gataattgta aactcacaaa acgagatgtc tactgcttca atcttgatgc aggcgagctg
      361 gagaatatca ccaatactag cgataaactc gagtatgaag taatggtagg tgaaaaatat
      421 atagcctggt ttgagtcgtc gcgttcagac cctgatcgtg ttgtttttat gaataggaca
      481 acaaaaaata aaaaatatat tgtacgcgac tccctgagga tcagaaatgt atcgtatgcc
      541 ctttcaggag aatatttttt atacaggaag tttgtttcga aagataatat ggtttcccat
      601 gagctatggg cgtacaatat tgaaaaagag agtgagatgc agatttctca aactgtaccg
      661 tatacccatc caatagagta tatcagcgat ggcagaatgg ttttttattc cacaacagtc
      721 aataaaaaga atgggagtga tatcttctca ttcaacctag agacgcttga gcaaaaaagt
      781 gtttgcacag aagtaggtgc ccaaaaaaac cttgatctga agggggatat ccttgtttgg
      841 gaggaccata ggaagggttc atgggattct gatatatatg gatatagaat ttctgagggc
      901 ctagtattta caattgatac atccagatat tgtacctatg ccccaaagat ttcaggaaat
      961 tgcgttgttt atagggcagt ctacgaatcg ccgcctgacc ggaaaagcac atcctatatt
     1021 tatctggctg atacctctag caatccgatt acgccaatca agctttcctc gtcagaaacc
     1081 ccaaaagata atcttatgat tgatggggat ttcctattgt gggttgacta taaaagaaag
     1141 aatcgtccta ttctcgaaga atatacaatt tggggttatt gtgtatcaga tcgaatagaa
     1201 tttgaggcta tttcaggtaa aacaagcaat aattggcttg tagatttcaa aaagggaatt
     1261 ttcctttggt cggacagcga ggacctgaat tattttgaca agatccatat caacaccatt
     1321 ggtcgctaag ttacaaaaac aatactgaaa ccttaaccac cagaaatgcc atgatccatg
     1381 ccagggcaag actgtatgca acctgaaagg tggcccattt ccatcctgct tctgctttgg
     1441 ttgcggctat ggttgcaagg catggcgtgt agaggttgag aaacaccatg agcccaagtg
     1501 ctgatgatga agaataccac ttgcttatat tatcatggct cccatcacca agttgtccaa
     1561 agactgctat cacgacttct tttgccacaa tcccgaaaag aagcgcaaca gatgcacgcc
     1621 agtcgaggcc aagtggcgta aatgcaaatg aaatgaagtg gccaatctgg cctattatgg
     1681 tgttctcaag cctgacgccc caggggatgt ttgatgcaac ccagaaaacc agcgacccaa
     1741 tgagaataac tcctccagct ttcttgatga aatgttttgc tctgtcccag gtgtggatca
     1801 ggacgctcct cacagttggc accctatatg gaggcaattc aatgaggagt ggggtcggtg
     1861 gctggttttt gaacaaaact aatccaaaga atttcgctgt tccgacagac agtgtcatgc
     1921 tgagagcaac tacggcccag acgaaattgc ctgcgcttgc accaaaaaat gcgctggcaa
     1981 caaagacgag tacctccatc cttgcagaac aggtcacaag tggcgctgac aggattgtta
     2041 ttatcctgtc ccttttgctt tccagtgtcc ttgttgccat gactgccggc actgagcatc
     2101 caaaacctat cacaaatggc acaaatgcct tcccggaaag acctattctc gacatcaggc
     2161 catccattac gaaagctatc cttgccatgt aaccggagtc ttcaagtgca ctcaaaaaca
     2221 caaacaggat gaagatgtta ggaaataggg caagcactcc gccaacgcca tttattattg
     2281 catcgtggag tactccgcca agccatggca cggactgcag ggtctgttgt atccatgccc
     2341 ccagcttgtc caaaccagtg ccaattagct ctccgatggg atttcccaaa aggaaagtca
     2401 gctggaaaac accccacagg atcacaagaa aaaagataat gccaaagaat ttgtttatca
     2461 ggacggaatc aatggcatcg gtgacctccg ccttcccctg aggtgaagag atggtgacca
     2521 tttttgccaa cccgtttgct gcagcatacc tcctgtcggc aacaagggtt tcgatgtcat
     2581 cgccatatat ttttgtgagt ttctttattt ccatggccac gatctcgtgt actttttcgc
     2641 tgtgtgctat ggaatctagt tcatgctcta cctgtgggtc tttctccagc agtttgtggg
     2701 cgaaaaagtg tgttttgtag cttgtttttg acacttcatc cgagcttatt gcgcactcta
     2761 tttttttggc cgagacctct atgtctgtgc cgaaatcaac cctgtagtgg cttttttgtt
     2821 ttgatttgcc aacttgaatt gcggtttttt ttaattctgc catgccccgc ccggttgagc
     2881 caacagtctc tacgacaggc acgccaagta tcctggacag ttttgcggaa tcgatcttta
     2941 tgcctttttt ttgtgcctcg tcctgcatat taagtgctat aacaagtggt acctcaagct
     3001 ctatgagctg gagcgtcagg aaaagactgt gttcgagggc cgttgagtcg acaacgttga
     3061 ttgcgacatc gggagtgttt ttgaccagaa agtccctggc aacgatttca tcctctgtga
     3121 atgcagataa ggagtatgtt cctggcaagt ctactatttc agcttgtaca ccgtctacaa
     3181 gcatcctgcc agtctttttt tcgacagtga cacccggcca gttgccaacg gcctgcgttg
     3241 agcctgtcaa tgcgttgaaa acagatgttt tgccagtgtt cgggtttccg gccagtgcaa
     3301 ttgtcaggtc gttgtttgcc aaaccctttt ttccttatct gtccctgaac ctgaggagcc
     3361 gcatgccatt aagggaaaca aggaatgtca ctccaacatc ggccagcacg gcaagccaca
     3421 tggttccaaa accaagcagc acaacgacaa aaaagcttgc ctttactgca agcgaaaagg
     3481 ctatgttctg gtagatggtc ctcatggcat gcctggaaat gcgaaaagta aacgggagtt
     3541 tgctcaaatc gccactcata agcacaacat cggatgtttc cattgcttgt cctgttccag
     3601 tgcttcccat tgcaatgcca actgatgact ctgcaagcgc aggggcatca tttattccgt
     3661 caccaaccat ggcgacatgt ttttctgagc ccatcagatc ttttattgca tcaagttttt
     3721 tctccgggag aagctctgaa tatacctttt ctattcccac ctggtctgca atcagttttg
     3781 ctgttgcctt tgcatcacca gtaagcatga cgagattttc gatgcccatc ttttttagct
     3841 tgtcgactgc caccttgctg gtgtcgcgaa ctgtgtctgc gacggttata aatccggaat
     3901 attttccttc aatgctcaca agcagtgggg tgtaaccctg gattgttgcc agattgatct
     3961 cttcgcaagt cagaggatca tgggggacat tgctgtcaaa aaatgaatgg ctacctacaa
     4021 aaacgctgtc gccgttgacc ttcccactga caccctttcc actcagggcc attacgtttt
     4081 ctgcgggctt gtaatgggcg tggatgcccc ttgccctggc ttcttccagg attgcctgtg
     4141 ccagggggtg ttcactcctg ctttcaactg atgatgcaat tgcaagcaaa tcgttgcaat
     4201 gcttgcagtc gccgccctca cagctaaggc agttttcaga ttttactttt atgactcttg
     4261 gtttgccttc cgtcaatgtc ccggttttgt caaatgcaat tatttttgtc ctggagagtt
     4321 cttccaggta ggccccgcct tttatcaaaa cgccattcct ggctgcatta ctaatcgcgc
     4381 ttacaattgt tactggaaca cttatgacca gcgagcaggg gcaggcgaca acgagcagct
     4441 ccagcgccct gtagagccag ccctgggtgc cattggccag gttgaggaat ggcgcaccaa
     4501 aaaatattgg aggcacagaa gcaaccagta ttgccagcac gatgacgacc ggagtgtaat
     4561 attttgcaaa cctgtctacg aacctttcgg cgggtgcttt tctctcctgg gcttcctcaa
     4621 caagcctgat catcctgctg attgtgttgt cagacgagat gtttgtgact tcgatcgtca
     4681 aggcgccctc gccattnnnn nnnnnncacg gtcttcctcg cacttgtaaa ccgggatgct
     4741 ttcacctgtt atcggcgcct ggtcaactga cgagcttcct gatataacga tgccatccat
     4801 tgggatcttc tcgccaggct tcactacaat tctgtcgcca acaacaagct cttcaactgg
     4861 cacctttttt tcttcagggc cacagaatgg gcatgggccg ccattatagc catctttccc
     4921 catgtgttct tcacagtcaa cgcatggcct caatacgaga gcgtcgtttg gagcgacttc
     4981 catcagcttc ctgattgcat ccctcgactg ctccatggtg tagccttcga gtgcctcgcc
     5041 gatcatgaaa aggaccatta ccagtccagc ctcggtgtat gcgccgatca caacaccgcc
     5101 aacacctgca ataaccatca gcaggttcat tgttatttcg tgatttgaaa agaggttttt
     5161 gaatgccgag ataaacaccg ggtagcccgc aatgatcaaa gccgctatgg ccatatactc
     5221 gaaaaccggt gtgccaatac ccaggaaggg aagcaactcg tcaaagacga gtcctggaaa
     5281 aacaagaaca aatccaatca gcgccagtat tgtatcgcgg tctccaagga gaaactggat
     5341 aaatccgata aaacccttgt atcttttatt tttcttggga gtatttgagg ttttgtcttc
     5401 ctccactatt tcgtaaccca tatccttgat ggtttttgca accacttcgt gaggcatagt
     5461 tccttcgact attatttttg aagccgagaa aacaagcctg cacgagtcga cgccatctat
     5521 tctggaaaca gttttttcaa gaccttttgc acaatccggg caagaaagac cggatagcct
     5581 gaattccatt ttattttttg attgttcttg ttttgccatt atgtttgtca ccaaaagaag
     5641 agtactcctg ttgagcatgg gagcaagata ttcgcatacg aggagcagtt aaaagatgta
     5701 tgtttattat acagtagaac attctacatc tcaaatgggg cagttttcat ctttttgctg
     5761 ctggagcagg catttggagg tttcccggca gatgatcttt gaggccatgc ctcttcctat
     5821 ggccagcctg acacatttta ccaatacaag aacggggcca gagaagtcgg accttaggac
     5881 cttgatgcaa gcacctgctg taatgccaag atctgcgagt ctcttacgca tgctacaccc
     5941 agggatgctt actatctcta tttcatcacc tggcctgaag cttgtcagtg gccttggcaa
     6001 gctagtttcg atctccattt catctacctc tttatatatt gcgaatattt tgcaatatca
     6061 atataacatt tatacagtta agtgcaacca aagataaaaa cattggatta gacaatattt
     6121 ctgcggcttg atgaaacaca tgtccttttt gcccttaacc aataggcaaa ggattgtatt
     6181 ccaaaaatat aagcatggtg caatattatt tgccaattta tgacccttta atataccttg
     6241 ttgacatcct ccccgttttc agtagactta caaactcgtt ggcgatgaag tgcctcttga
     6301 cagagtgacc tgaatctgct aacctgatac gagcaagcgg cgaaaggggg aaccccgagg
     6361 ccttttgcga gcggcagttt gaaaactaga tagtgatgga acccttcaat tcgataaata
     6421 attggtgtgt taaagacatc ttcgatgtca gcacataagt tgctcgaaga gtttgatcct
     6481 ggctcaggat gaacgctggc ggtgtgcctt atacatgcat gtcgaacgag gcgtagcaat
     6541 acgtctagtg gcaaatgggt gagtaataaa taggtaacca acccttaaga cggggatacc
     6601 ccatcgaaag atggaataat acccgataat gtcccttccg gtgcgctgga ggggaagaaa
     6661 gatggcgcaa gctatcactt ttggacgggc ttatttccta tcagctagtt ggtgaggtaa
     6721 cggcttacca aggctacgac gggtagctgg tctgagagga cggtcagcca cattgggact
     6781 gcgacacggc ccagactcct acgggaggca gcagtcggga atattcggcc aatgcgcgaa
     6841 agcgtgaccg agcgacaccg cgtgggcgac gaagatctta ggattgtaaa gcccttttgc
     6901 cagggaagaa tacgctggag acaatatcaa tagcgtttga cggtacctgg cgaataagcc
     6961 gcagcaaact acgtgccagc agctgcggta atacgtaggg ggcgagcgtt atccggaatt
     7021 actgggtgta aagggtccgc aggcgacttg ttaagttaag agttaaatct caaagcccaa
     7081 ctttgaggcc gctcttaaga ctggcaagat agagtgtaga agacggaagt ggaattcctg
     7141 gtggagaggt aaaatccgta gatatcagga ggaacacctg tggtgaagac ggcttccgag
     7201 tctataactg acgctcatgg acgaaagcta ggggagcaaa caggattaga ta
//
LOCUS       DAOSRI010000055         7235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9614_length_7235_cov_5.488022, whole genome shotgun sequence.
ACCESSION   DAOSRI010000055 DAOSRI010000000
VERSION     DAOSRI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9614_length_7235_cov_5.488022"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>1716)
                     /locus_tag="PK717_02590"
     CDS             complement(158..>1716)
                     /locus_tag="PK717_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOR46583.1"
                     /translation="IEALHPFHWGFEFDEVINARGGFDAIITNPPWDIFKPNGKEFFE
                     NFSDVVTRKKMTIKEFEEEKSKLLKDPAVLKAWLEYSSSFPHVSSFFRSSSQYANQIS
                     IVDNKKQGTDINMYKLFTEQCFNLLRQGGYCGIVIPSGIYTDLGTKQLRQMLFDKTQI
                     TGLFCFENRKGIFENVDSRFKFVVLTFEKGGKTAQFPAAFMRHEVSELQAFPKQGSIE
                     IDVGLVKRLSPDSLSVVEFKKDIDIKIAEKMLKYPLLGDEIPDKWNVKLATEFHMTND
                     SYLFKTEPGPGRLPLYEGKMIHQFDHKYAKPKYWIDEAEGREALLGKSEDKGQKLDYM
                     SYRLILRKIARNTDCRTLITSIIQKNTFFSDSTNIFTINKSRITNQTLLFNTSLLNSF
                     VLDYFIRSSVTANINIFRLYQIPTPRFTEKDPQFMPIVSRAAKLICTTPEFDELASEV
                     GLGSHKNGVTDPAERAKLRSELDGLVAHLYGVTREEFQHILSTFPIVAQEVRDAALRA
                     YDDVAKGKIK"
     assembly_gap    1717..1816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1817..3738)
                     /locus_tag="PK717_02595"
     CDS             complement(<1817..3738)
                     /locus_tag="PK717_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR46584.1"
                     /translation="MSLDLEKIKNNLENFDFSELFIGQLGWSCPNSTGLKNISTNAGS
                     FDYLQIAELSGVFVLHLPQKTDIPDPKTRQQISKKLSELYPENILIFTDSAKTQSLWL
                     WVKTDNGKAYPREHIFIKGQPADLFLSKLKSLHFDFSDFSDTGHVALSEVTKRLKDAL
                     DVERVTKRFYQDFQKMHLEFLGLIEGINDEKDRRWYASVLLNRLMFIYFLQGKLFLDN
                     GDARYLQNKLEQSKAKGKNIFYEVFLKALFFEGFAKPEDKRSKETNDLLGKIKYLNGG
                     LFLRHRIEIEYKDIKIPDIAFENLLKLFSSYSWNLNDTIGGKADEINPDVLGYIFEKY
                     INQKAFGAYYTRPEITEYLCSQTIHKLVLDSINSPGHPGVFPAINFESIDELILRLDA
                     PLCKKLIYEVLPKMRLLDPACGSGAFLVQAMKKLVDIYSVVIGRIEFLSDSNLKAWVE
                     DIKTKHPSINYFIKKKIITDNLYGVDIMEEAVEIAKLRLFLALVSSVSSASELEPLPN
                     IDFNILPGNSLIGLMKVDDVEFENRNKNLFRKSYQEILNEKNRQIEIFRHATTYAEDL
                     ASLRDGIAVIKQNAQKTLDEILKSEFDNLGIKFEQATWDEAKNKEGKPVKRAISIADI
                     EALHPFHWGFEFDEVI"
     gene            complement(3752..7102)
                     /locus_tag="PK717_02600"
     CDS             complement(3752..7102)
                     /locus_tag="PK717_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOR46585.1"
                     /translation="MPNIYDNIEKKLIDALKETAKLSYKADFCIAYFNLKGWEHLSEI
                     VNVWDGCDGNCARLLIGMHCSPEEEIRRINSINKNGETLDRSKAIVLKQKTIQDLKNQ
                     LIQGIPTNRHEEALKNLSIQLQEKKVIIRLLTKGSLHAKLYLLYRNDPVSPVIAYLGS
                     SNLTSSGLSNHGELNIDITDQDACNKLASWFQQRWEDNFCLDVSDDLISAINESWVNG
                     DLPYNIYMKIIYHLSKEARSGIQEFSIPTDFGNRLFAFQKAAVQIATQYLNKRGGVFI
                     GDVVGLGKTLMATAVARIMEDDLGIETLIICPKNLENMWEDYRLKYRLRAKVLPFSKV
                     LTELPTMQRFRQVLIDESHNLRNPKGKRYKVIKEYIEKNDSKCILLSATPYNKSFIDL
                     SAQLRLFIQDDDMLGIKPEKQIYQDGGITKFVQKYGFAPESISAFEKSDNADDWRDLM
                     RLFLVRRTRGFIQQHYAKTDPSNGRKYLDLEDGQKSYFPARQPKTMKYSFDEAMEDQY
                     SKLFSQEVVDIISNLHLARYGLGSYLSDIKNIKLNTEEKEIIENLSRAGQRLKGFCRT
                     SLFKRLESSGYVFIKSVERHILRNYVFLYALENNLPLPIGTQDLGIFDPVLSDQEYDY
                     FNDTNDNEFDNEVQVKYEKGYFEEEASNIYRFYKNKGGKSFKWIRSSLFKTLLMQHLS
                     EDNQALELLLSKIGTWDPKKDNKIKNLFELITKTHPDKKILIFTQFADTVAYLQTELG
                     KKGLEKFAGVTGKSEDPSDLVWKFSPESNNAKDRINSNEELRILIATDVLSEGQNLQD
                     CSIIINFDLPWAIIRLIQRVGRVDRIGQRAENILAYSFIPADGVEKIIKLRSRLAQRL
                     SENAEVIGTDEQFFEDGLNKGQMEDLYNEKAKILETETDSEVDLSSYAYQIWKSAISA
                     NPDLENKIKKLPDVVYSTKMGSKSQSFQNGVVVYIKTPDGTDSLALIDDKGQIITQSQ
                     HDILKLAECSINTPAMPRLENHHDLVELAVKKIMEQNEHDGNSLGPKRKARHKTYMRL
                     KHFMQNSVTAVIDREALNKAIEEIYKYPLQSMAEETINRQLRSGIGDKELAEMVIVMS
                     QEERLCAISDEPMDFEPRIKCTLGLIGQ"
BASE COUNT         1867 a         1471 c         1355 g         2442 t
ORIGIN      
        1 atctttatgg tgagtaacga ttgattttct tttaccaatt catgcacttc aatttgtttt
       61 ggccgatatt gaagtcttgt tcctttttct ttcatcagat tcctcaatgt ggcgggtttt
      121 aaagacaaca tcctgcctca atattcattt tatgtttcta tttgatcttg cctttggcca
      181 cgtcgtcata ggcacgcaaa gcggcatccc tgacctcctg ggcgacgatt gggaatgtgg
      241 agaggatgtg ctggaattcc tcacgggtga caccgtaaag atgggccaca agcccgtcca
      301 gctctgagcg gagctttgca cgctctgcag gatcggtgac gccgtttttg tgagagccaa
      361 gcccaacctc gctggctagc tcgtcaaact cgggggtggt gcagatgagc ttggccgcac
      421 ggctgacaat gggcataaac tgagggtctt tttcggtaaa gcgaggggtt ggtatttgat
      481 aaagtctaaa aatatttata tttgcagtaa ctgaagaacg tataaaatag tccaaaacaa
      541 atgagtttaa taagctcgta ttaaacaaca aagtttggtt tgttatccgt gatttattaa
      601 ttgtaaatat atttgtagaa tcactaaaaa atgtattttt ttgtattatt gatgtaatca
      661 aagttctaca atctgtattt ctagctattt ttcttaatat taacctataa ctcatgtaat
      721 ccagtttctg acctttgtcc tcgctttttc caagcagagc ttctctgcct tcggcttcat
      781 ctatccagta ttttggtttt gcatacttgt ggtcaaactg gtggatcatc ttgccttcat
      841 aaagaggcag tctgccaggg ccaggttctg ttttgaacag ataactgtcg tttgtcatgt
      901 gaaattcagt tgcaagtttc acattccatt tgtcgggtat ttcgtctcct agcaaagggt
      961 atttcagcat tttctcagca attttaatgt ctatgtcttt tttgaactcc acaacagaga
     1021 gcgaatcagg cgagagcctt ttgaccaggc caacgtctat ttcaattgac ccctgctttg
     1081 ggaatgcctg gagctctgac acttcatgcc tcatgaaggc agcagggaat tgggctgttt
     1141 tgccaccttt ttcaaatgtt agcacgacaa atttaaatcg tgaatccaca ttttcgaata
     1201 tgccttttct attctcaaag cagaaaagcc ctgttatctg tgttttgtca aagagcatct
     1261 ggcggagctg tttggtacct agatcggtgt aaattccgct tgggatgaca attccgcaat
     1321 aaccgccctg ccgtagcagg ttgaaacact gttctgtaaa gagtttgtac atgttgatgt
     1381 ctgtaccttg ttttttgttg tctacaatgc tgatttgatt ggcatattgg gaggatgacc
     1441 tgaaaaacga gctgacatga ggaaaactac ttgaatattc aagccaggcc ttcaaaactg
     1501 caggatcttt gagaagtttt gacttctctt cttcaaactc cttgatagtc attttcttcc
     1561 ttgtgacaac atccgaaaag ttctcaaaga attccttgcc atttggtttg aaaatatccc
     1621 agggcggatt tgtgattatt gcatcaaacc ctcctctggc gtttattacc tcgtcaaact
     1681 cgaatcccca gtggaaaggg tgcagggctt caatgtnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnttta ttacctcgtc aaactcgaat ccccagtgga aagggtgcag
     1861 ggcttcaatg tctgcaattg atatcgcacg cttgacaggt ttgccttcct tgttttttgc
     1921 ttcgtcccaa gtggcctgct caaatttgat gcccaggttg tcaaactcgc ttttgaggat
     1981 ttcatccagt gttttttggg cgttctgctt tatgactgca atgccgtccc tcaggctggc
     2041 aaggtcttca gcgtaggttg ttgcatgcct gaagatttct atctgcctgt ttttctcatt
     2101 aaggatttcc tggtaggatt tcctgaaaag gtttttgttt ctgttctcaa actcaacatc
     2161 gtcaactttc ataagcccta tcaaggaatt gcctggcagt atgttgaagt ctatgtttgg
     2221 caggggctca agctccgatg cgcttgacac agaggagaca agggcaagga aaagcctgag
     2281 tttggcaatc tccacggcct cctccattat gtcaacgccg taaagattgt ccgtgattat
     2341 ttttttcttg atgaaataat tgatgcttgg atgttttgtt ttgatgtctt ccacccatgc
     2401 tttcaaatta gagtctgaaa ggaactctat tctgccaatc acaactgaat aaatatccac
     2461 aagctttttc atggcctgga caaggaaagc cccggagccg caagcaggat caagcaggcg
     2521 catttttggc aggacttcat agatgagctt tttgcacaaa ggcgcatcca gacgcaggat
     2581 gagttcatca atgctttcaa aattgatggc agggaacacc ccggggtgcc ctgggctgtt
     2641 tatgctgtcc agcactaact tgtggattgt ctggctgcaa aggtattccg taatctctgg
     2701 tcttgtgtag taggccccaa aagctttctg gttgatgtac ttttcaaaga tataaccaag
     2761 gacgtctgga tttatttcat ctgctttgcc tcctatggtg tcattgaggt tccaggaata
     2821 ggaagaaaaa agcttgagca ggttttcaaa agcaatgtcc gggattttta tatccttata
     2881 ttcaatttct atcctgtgcc ttagaaacaa tccgccgttt agatatttta tcttgcccag
     2941 caaatcattg gtttcttttg accttttgtc ctctggtttt gcaaaaccct caaaaaacaa
     3001 tgctttgaga aacacctcat aaaagatgtt tttgcctttt gctttggact gttcaagctt
     3061 gttttggaga taacgggcat ctccgttgtc aaggaacagc ttgccttgga gaaaatatat
     3121 gaacatgagc cggtttagga gcaccgaagc ataccagcgt ctgtcttttt cgtcatttat
     3181 accttcaatc aggcccaaaa attccaggtg cattttttga aaatcctgat agaatctttt
     3241 tgtgacccgt tcgacatcga gagcatcttt aagccttttt gtcacctctg aaagtgcaac
     3301 atggcctgtg tcggaaaaat cggagaaatc aaaatgcaaa gatttaagct tggaaaggaa
     3361 aagatcggca ggttgacctt taataaaaat atgctctctt ggatacgcct tgccgttgtc
     3421 agtctttacc cagagccaga gactctgggt tttggcgcta tctgtaaaaa taagaatatt
     3481 ttctggataa agctctgata gttttttgct tatttgctgt ctggtctttg ggtcaggaat
     3541 gtctgttttt tgtggaaggt gcaacacaaa cacccccgat agctctgcaa tttgcaagta
     3601 gtcaaaactc ccggcattag tgcttatatt tttcaaaccc gtagaatttg gacagctcca
     3661 cccgagttgg ccaataaaaa gttctgaaaa atcaaaattc tcaagattat tttttatctt
     3721 ttcaagatca agcgacatac ttcccctcct tttattggcc aatcagtcca agcgtgcatt
     3781 ttattctggg ttcaaagtcc attggttcat ctgatatggc gcaaagcctt tcttcctggc
     3841 tcatgacgat aaccatctct gctagttctt tgtcccctat tcctgagcgg agctgtctgt
     3901 taatcgtctc ttcagccatg ctttgcaaag ggtatttata gatttcttca atcgccttgt
     3961 tcaaagcttc tctatcaatt actgctgtaa cgctgttctg catgaagtgc ttgaggcgca
     4021 tatatgtttt gtgtcttgct ttcctttttg gaccaagcga attaccatca tgttcgtttt
     4081 gctccataat tttcttcaca gccaattcaa caagatcatg gtggttttcc aaccttggca
     4141 tcgccggagt gttgattgaa cattcggcca acttcaaaat atcatgctga gactgggtga
     4201 ttatttgtcc cttgtcgtct atcagcgcaa gagagtctgt gccatcagga gtttttatat
     4261 aaacaacaac tccgttttga aagctttgtg attttgaacc catctttgtt gaataaacaa
     4321 catccggcag ttttttgatc ttattttcca gatcaggatt ggcactaatt gcacttttcc
     4381 aaatttgata agcatatgac gacaaatcaa cttcggagtc tgtttctgtc tccaggattt
     4441 ttgctttttc attgtataga tcttccattt gccctttgtt gagtccatct tcaaagaatt
     4501 gctcgtcagt tcctattact tctgcgttct cactgagcct ttgagcaagt cttgatctca
     4561 atttgataat tttttccact ccatctgctg gaataaatga atatgctagt atattctctg
     4621 ctctctggcc tattctgtcc acacgcccca ccctctggat gagtcttatt attgcccagg
     4681 gaaggtcaaa gttgattatt attgagcaat cctgaagatt ttgaccttcg cttagaacat
     4741 cagttgcaat caagatcctc agttcttcat ttgaatttat tctgtctttt gcgttgttgc
     4801 tttcagggct aaatttccaa accaaatctg aagggtcctc tgatttacct gtaactccgg
     4861 caaatttttc cagacctttt ttgcctagct ctgtttgaag gtaagcaact gtatctgcaa
     4921 actgtgtgaa tatcaaaatt ttcttgtcag ggtgagtttt ggttatcaac tcgaacaaat
     4981 tctttatctt attgtctttt tttggatccc atgttccaat tttactcagt agtaattcaa
     5041 gagcctgatt gtcctcacta aggtgttgca ttagaagagt tttaaacaaa cttgagcgaa
     5101 tccatttaaa ggatttgcct cctttgtttt tataaaatct atagatattg gaagcctctt
     5161 cttcaaaata gcctttttcg tacttgacct gaacttcatt atcaaactca ttatcgttgg
     5221 tatcattgaa ataatcatat tcttggtcag acaagacagg atcaaaaatg cccaggtcct
     5281 gggtgccaat tggcaatggg agattatttt caagggcata taaaaacaca taatttctaa
     5341 gaatgtgtct ttctactgat tttataaaaa catagccact actttccagc cttttaaaca
     5401 agcttgtcct gcaaaaacct tttaatcttt gacctgctct tgaaaggttt tcaattattt
     5461 ctttttcttc ggtattcaat tttatattct ttatgtctga aagatatgaa cctaatccat
     5521 aacgggccaa atgaaggtta cttataatat ctacaacctc ttgtgaaaaa agttttgaat
     5581 actggtcttc catcgcttca tcaaagctgt atttcatcgt ttttggttgt cttgctggaa
     5641 agtatgactt ttgaccatct tccaaatcaa ggtatttcct tccatttgag ggatctgttt
     5701 ttgcataatg ttgttgtatg aaacctcttg tgcgcctaac taaaaaaagc ctcatcaagt
     5761 cacgccaatc atctgcattg tcactttttt caaaggctga tatagactcc ggggcaaaac
     5821 catacttttg cacaaatttg gtaataccac catcttgata aatttgtttt tctggcttta
     5881 tacctaacat atcatcatct tgtatgaata gtctaagctg ggctgaaaga tcaataaacg
     5941 atttattgta aggtgtggct gataagagga tgcatttact atcatttttt tcaatgtact
     6001 cttttataac cttatatctt ttaccctttg ggttcctcaa attatggctc tcgtctatca
     6061 aaacctgtct aaatctttgc attgtaggta attcagtgag tactttactg aatggtaaca
     6121 cttttgctct gagtctatat ttcagtctgt aatcttccca catattctca agatttttag
     6181 ggcaaataat taatgtttca atacctaagt catcctccat aatcctagcc acagctgtag
     6241 ccataagtgt tttacccaag ccaacaacat caccgataaa aacaccacct cttttattaa
     6301 ggtattgagt ggcaatttgt accgcagctt tctggaaagc aaacaatcgg ttgccaaaat
     6361 cagttgggat tgagaattct tgaatacctg aacgggcttc tttagaaaga tgatagatta
     6421 ttttcatgta gatgttgtat ggcaaatctc catttaccca gctctcattg atagcagata
     6481 tcaagtcatc gctcacatcc aaacaaaagt tgtcttccca tctttgttga aaccacgaag
     6541 caagcttatt gcaagcatcc tggtccgtga tatcaatatt gagctctcca tgattcgata
     6601 aaccagagga agttaaattg ctgctgccaa gatatgcaat aacaggtgat actggatcat
     6661 ttcgatagag aagatacaac tttgcatgta aacttccttt tgttaaaagc ctaattatga
     6721 cctttttttc ttggagttgg attgataagt tttttaatgc ctcctcgtgt ctatttgttg
     6781 gtataccctg aataagttgg ttttttaaat cttgtatagt tttttgtttt aaaacaattg
     6841 cttttgacct atcaagtgtt tcaccatttt tattaattga gtttatcctc cgaatttctt
     6901 cttctggtga acaatgcatg ccaataagca atcttgcaca gtttccatcg caaccatccc
     6961 aaacattgac aatctcggaa aggtgctccc aaccctttag attaaaatag gctatgcaaa
     7021 aatcagcttt atatgataac ttagctgttt cttttagtgc atcaatcagc tttttctcaa
     7081 tattgtcata tatgttcggc aaagttttct cctttaaaaa ttcccctaga ataaatgaga
     7141 aatcaaggtg tattcaaata agtacctttt ttggaattgt cagaatcatt tgttgaattc
     7201 accagaccaa catctggatt gttcaatctt cctga
//
LOCUS       DAOSRI010000056         7203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9680_length_7203_cov_4.557359, whole genome shotgun sequence.
ACCESSION   DAOSRI010000056 DAOSRI010000000
VERSION     DAOSRI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9680_length_7203_cov_4.557359"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK717_02605"
     CDS             <1..998
                     /locus_tag="PK717_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46586.1"
                     /translation="NPIGYKDPSGFSPSCGGHSSFKGNNDTGESSMPEDNNDILSTND
                     PSYYIQDNAMEETWKFCLENNIFKDTKEDMEKFVSLWNQANNVRTRMQKYGGTDDKYD
                     MKIAFWTCYWNDKAKLGLEGTELVSMANLMKGIMYEESTMGATAHHNEGLMQVNTFGA
                     MSETHWVEDGFYKKWNGRLGEDDNAGIYAGIAHWCMDYSGFAFGPDQLNRDFNDFIGD
                     IKSGKNPIHNKSIWKKATYRYGAGSDNDQRIKAIDPNLNYSDLVFDLVEKGRDPHFHQ
                     EGSRWVRNTSQVWNTKRGTGINMYNPVIYEEIYKRQDLTAKQKNELMNDANIDLP"
     gene            1013..2350
                     /locus_tag="PK717_02610"
     CDS             1013..2350
                     /locus_tag="PK717_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46587.1"
                     /translation="MFKMRSHYYGILKITALAIIIAQSACGGNNLNKIESIPLVNNNS
                     EKVKTISPQEYNPESYFSFIPTEKLSEWAPKNNKTINEKISAQWVPKKFFGKNKLEMR
                     WSHQITTEGSNPRYSPTISLPTGWLINYQAKADGQKLHAMINPENGEINSLDETLCGG
                     QTDGKYVFGYSQTVVGKAVCWDLNNQEIYWEGMMGYLDNPLMIVNDKLIAINGDDLMT
                     NRVMSGDPETGKILWMLEGKQGLNISSWTIQGNKLWAVIENGELYEINLEDGHHKKIA
                     LSNKSQPLGIESSNNCIWIFYSDGYLISYDADSFSTKSEARIGRELGEPELLELNLDS
                     FDNHLLLSIKSYKTYKKEYHLIYETNPPKVKMLEQIENEPVCWPPCPTWVVLNGALVN
                     QSNGQIKEVDPSTLENILWIDEKDVGPNPRVNILDWRGVCVISDTKIMCFGPK"
     gene            2632..3231
                     /locus_tag="PK717_02615"
     CDS             2632..3231
                     /locus_tag="PK717_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46588.1"
                     /translation="MNKLNIVKTHCITLFGKTDKYDIVLKPLNDEHLPLLYKWCADPE
                     VLYWTEGGEDADLSYDKETVHAIYGGVSQNAYCFLIEANGVPIGEGWLQKMNLPEILA
                     MYPKTLDVRRIDMSIGEKDYWNQGIGSQFVHMMVEFAFASEHVDVLHCICGGYNKRSQ
                     RVFEKNGFTLMQMDGPPQPQEEQIEYHYILTAPEYFRQK"
     gene            complement(3256..3513)
                     /locus_tag="PK717_02620"
     CDS             complement(3256..3513)
                     /locus_tag="PK717_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HOR46589.1"
                     /translation="MKYKNAADILPDELLQEVQKYISGESLYIPLDKERKKWGDNSGA
                     RRFFAQRNEEIRYKFSHKVSVDELADEYFLSPETIRKIVYK"
     gene            complement(3532..4572)
                     /locus_tag="PK717_02625"
     CDS             complement(3532..4572)
                     /locus_tag="PK717_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46590.1"
                     /translation="MKTINEKLIDWALAKIEKEYKDDICLLVAHDTLKLEKDMAGMSM
                     SYFVPATENAYGFAKTFIINGIGYDLFPMSWGRLERLSNLDDNNTSCLGDVQILYCRN
                     PEDEKRFVALQTKLQENLQNREFMFKKALEKMNVAMEIYQTMMFEESIGKVRKASGYI
                     ADFLSIAVAFSNQTYFKNGQTNQISDLSAMKSIPKDFIGTYEAIVNADSVDELKKLCH
                     KMIYNTRKFLNAKKPCTKNRNYNKNFKDLANWYQELSYTWRRIYYWCDQNGKVKSFMW
                     GCFLQSELDIVKEEFGLDEMDLLGKFSSKDLTVFHNHTEQLEKYIVGKIEEQGVILDK
                     YDSVEDFIKKNS"
     gene            4881..7064
                     /locus_tag="PK717_02630"
     CDS             4881..7064
                     /locus_tag="PK717_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOR46591.1"
                     /translation="MKSNRILIIILTIILVLVPFGPAPRKTDAASYYIGGRLVPGPLP
                     PSFYAPLPPLNVRLNLNYAGSANRWQTLDFAKPVMCASQKVPVIAYVHGGWWLGGSKT
                     GAIYSMPARLAYQLGFAVVSIEYRLASSTNHHPNLINDCKLAIRYLRANADMLGIDPN
                     RIGIWGGSAGGHLVSLMGTAGDDDGLEGPGFEGYSSRPNAVVDQCGIEDLTKPWDNTS
                     RMVFGIFLGCAYNVCTDKAQEASPVYQASPDDPPIMIMHGDHDNVVSYTQAEEFARAL
                     KANGNNGSFIRIVNGNHGFGSYNGRDLVSPNWTVQNRLVISHLARFIEPGLMCDLNMD
                     GRVDQRDADELWIHLGESGFGPNAEPTVESWNPLADIHPDGKIDYKDIQSFCGQIAKP
                     VTVEKKVILPKNSQKIYEFDIELSNNGPLGVGNITITDSFPKELVFVSSSPQGALKGN
                     KIIIENLSLPANSKFTARILFKLSDGYKQPEEGAILSNSVSISSPDIFNIEDQVTFAL
                     PGIRIRKTSKSFNVAEGGLAVFQVTVTNPSARRLTGVEVTDSFPRELAFISSRPSGVA
                     SNKSVKFEVGDLGPGESRIFMLNFRLPKLASTGEGQTITNTASASSNELHPVFSSASI
                     FCPKKEQGGGELNIDIVWKGPDSKGRIAVGEEIEMTMQVTGGSAPYDISIDFGDRTRK
                     LFSLDGERAVEIAHSYSEPGEYAVKISVSDAYGRSRVVQRKIIVK"
BASE COUNT         2204 a         1436 c         1528 g         2035 t
ORIGIN      
        1 acaaccccat aggctacaaa gacccctctg gcttctcacc ctcctgtggt ggacacagca
       61 gtttcaaggg gaataatgat accggagaaa gctcgatgcc agaggataat aatgatattt
      121 tatcaactaa tgacccctcc tattatattc aagacaacgc catggaagaa acatggaaat
      181 tctgtttaga gaataatatt ttcaaagata ctaaagaaga tatggaaaaa tttgtatcct
      241 tatggaatca agcaaataat gttaggacaa ggatgcaaaa atatggcggt acggatgata
      301 aatatgatat gaagattgct ttctggacct gttactggaa tgataaagct aagttggggc
      361 ttgaaggaac agagctggtt tcgatggcga atttaatgaa agggataatg tatgaagagt
      421 caacaatggg tgccactgca catcataatg aaggcttaat gcaagtcaat acatttggcg
      481 cgatgtccga aacacattgg gtagaagatg gtttttataa gaaatggaat ggacgtcttg
      541 gcgaagatga taacgcaggt atatatgcag gtatagccca ttggtgcatg gattatagcg
      601 gttttgcgtt tggtccggat caattaaaca gagattttaa tgattttatt ggagatatca
      661 aaagtggcaa aaatccaatt cacaataaaa gcatatggaa gaaagccact tacagatatg
      721 gcgctggaag tgataatgat caaagaataa aagctattga tcccaatctt aattatagcg
      781 atctagtttt tgatctcgta gaaaaaggaa gggatccgca tttccatcag gaaggcagca
      841 gatgggtaag aaatacaagt caggtttgga atacaaaaag aggaactggc atcaatatgt
      901 acaaccctgt gatatatgaa gaaatatata agaggcagga tttaacagca aaacagaaga
      961 atgaattgat gaatgatgca aatattgatt tgccatgaag gaggtgcaag caatgttcaa
     1021 aatgaggtcg cattattatg gtattttgaa aattacagct ttagcaataa tcattgccca
     1081 atctgcttgt ggtggcaaca atttaaataa gattgagagc ataccgttgg tcaataataa
     1141 ttctgaaaag gtaaaaacaa tctctcccca agagtataat cctgaatcgt actttagttt
     1201 tattcctaca gaaaaattgt cggaatgggc tcctaaaaat aacaaaacca tcaatgaaaa
     1261 aatttcagca cagtgggtgc cgaagaagtt ttttggtaaa aacaaacttg aaatgcgatg
     1321 gtctcatcaa ataacaacag aaggatcaaa tcctagatat tctcctacga tcagtttgcc
     1381 aacaggttgg ttgataaact atcaggcaaa agctgatggc cagaaattgc atgcaatgat
     1441 aaatccagaa aatggggaaa taaacagttt agatgaaaca ctgtgcggtg gacaaactga
     1501 tggcaaatat gtttttggct atagccaaac cgtagtgggc aaagcagttt gttgggacct
     1561 aaacaatcag gagatttact gggaaggtat gatgggttat ttagacaatc cattaatgat
     1621 agtcaacgat aaattgatag ctataaatgg tgatgatttg atgacaaaca gagtgatgag
     1681 tggtgatcct gaaactggga aaatattgtg gatgcttgaa ggaaaacaag gattaaacat
     1741 atcaagttgg acaattcagg gcaacaaact ttgggctgtc atcgaaaatg gagagttata
     1801 tgaaatcaat ctggaagatg gccatcacaa gaaaattgct ctatccaaca aatcacaacc
     1861 tttgggaata gaatctagca acaattgcat atggatattc tactccgatg gttatttaat
     1921 ttcatatgat gctgatagct tctctacaaa aagtgaggca aggatagggc gagaactcgg
     1981 agagccagaa ttactcgaac taaatctgga ttcgtttgat aaccatctcc ttctctctat
     2041 caaatcctat aaaacctata agaaagaata tcatctaata tatgaaacaa accctccaaa
     2101 agtaaaaatg cttgagcaga ttgaaaatga gccagtttgc tggccaccgt gcccaacttg
     2161 ggtggtgttg aacggggcgc tagttaacca gtccaatggc caaatcaaag aggtggatcc
     2221 aagcacattg gaaaacattt tgtggattga tgaaaaagat gtcggaccaa atccaagggt
     2281 caatattctt gactggcgag gagtttgcgt tatctctgat accaaaataa tgtgctttgg
     2341 cccaaaataa ttaacctcca atcagtcctg gcgtcccctc cctccacttt tgaagatgcc
     2401 ctggcagaat tttgctggac gaatcagcat tgaagtttgc cgtaatattg aaaaaatcat
     2461 gccaattttc aggattgaga aaatatctcc tccacgtagg ggagaatgtt tcaatgtaga
     2521 agaaaactca aacatctagt gtataataat ttgtatgaga agaatgagtt tcattcaacg
     2581 ataaagaaag gcattttgaa actggcacga tccaattttg aaacatttgg ggtgaacaaa
     2641 ttgaatattg taaaaacaca ttgcataact ctttttggaa aaactgacaa atatgatata
     2701 gtcctaaaac ctttaaatga tgagcatctt ccgcttttat acaaatggtg tgcggacccc
     2761 gaggtcctat attggactga gggtggtgaa gatgccgatc tttcctatga caaggagacg
     2821 gtacatgcta tctatggtgg agtttcgcag aacgcatact gttttctgat agaggcaaac
     2881 ggcgttccca ttggagaggg ctggcttcaa aagatgaatt tgcctgaaat tctggccatg
     2941 tatccaaaaa cgcttgatgt ccgaaggatc gacatgtcta ttggcgaaaa agactattgg
     3001 aaccagggta tcggatcgca gttcgtacac atgatggttg aatttgcttt tgctagcgaa
     3061 catgttgatg ttttgcactg catatgcggg ggctacaaca aacgcagcca acgtgtgttt
     3121 gagaaaaatg gttttaccct catgcagatg gatggaccac cccaaccaca agaggaacag
     3181 attgaatatc actatatact tacagcacca gaatatttta gacaaaaata gggatcaaga
     3241 tatgtatccc aatccctatt tatagacaat cttccgtata gtttccggtg aaagaaaata
     3301 ttcatcggca agttcgtcta cgcttacttt gtgggagaat ttataccgga tttcctcgtt
     3361 tctctgggca aaaaatcttc ttgcaccaga attgtcgccc catttctttc gttccttatc
     3421 taatgggata tacaatgatt cacctgaaat atatttctgt acttcctgca gaagctcatc
     3481 aggcaatata tcggctgcat ttttatattt cattgtaaat ttgctggtgt cctatgaatt
     3541 tttctttata aaatcttcta ccgagtcata cttgtccaat ataacgccct gttcctcgat
     3601 ttttccaaca atatactttt caagttgctc ggtgtgattg tggaaaaccg ttaaatcctt
     3661 cgagctgaat ttgccaagca aatccatttc atcaagaccg aattcttctt tgactatatc
     3721 aagctctgat tgaaggaaac agccccacat aaagcttttt accttaccat tctgatcgca
     3781 ccagtaatag attcttctcc atgtatagct aagttcctga taccaatttg ccaaatcttt
     3841 aaaatttttg ttgtagtttc tatttttggt acagggtttt tttgcattca aaaatttccg
     3901 tgtattgtaa atcattttgt gacacaattt tttcaattca tctacagaat cagcattaac
     3961 aatagcttca taagtcccaa taaaatcctt aggtatactt ttcattgccg atagatctga
     4021 aatttggttt gtctgcccat ttttaaagta tgtctggttt gaaaaagcaa cagctataga
     4081 taggaaatcg gcaatatacc ctgacgcttt tcttaccttg cctatggatt cctcaaacat
     4141 cattgtttga tatatttcca ttgctacatt cattttttca agagctttct taaacataaa
     4201 ctccctattt tgcaaatttt cttgcagttt tgtttgtaat gcgacaaagc gcttttcatc
     4261 ttccggattc ctgcaatata gtatttgcac atcccccaaa caggatgtgt tgttgtcatc
     4321 cagattggat aacctttcca atcgtcccca ggacattgga aataaatcat aacctatacc
     4381 attaatgatg aaggtttttg caaacccata tgcattttcc gtggcaggaa caaaatagct
     4441 cattgacata cccgccatat ccttttccag tttcaaggtg tcatgcgcca ccaacagaca
     4501 tatatcatct ttgtattctt tctcaatttt tgccaatgcc cagtcaatta atttctcatt
     4561 tatcgtcttc atattgaaat tccacctttc agatagtttg ggttgcttac gaccaaattc
     4621 tatattggat aaaaaaatgt ttcaatgcca agatctcttc taaaaattct ctaccagaca
     4681 ttaaagatgg tattttttgc tttgtttata ttggtaatcc actattcatg gcatcgattc
     4741 cactcatgct gcaaattatg gcggtgtttt gtcaaaattg tttgttttta aaagtttcaa
     4801 ttcacaaaca ttcaaaaaga tgtcccaaaa caattctccc aagtttggtt tttttgatta
     4861 aaccgcataa aatccccagt gtgaagtcga atagaatatt gataatcatt ctaacaatca
     4921 tcctcgtgct ggtgccgttt gggccggctc ccagaaaaac agatgcggcg tcatattata
     4981 tcggtggcag gctggtgcct ggccctctcc cgccttcgtt ttatgcccct ttgccaccac
     5041 tgaatgtcag gctgaacctg aactacgccg gctcggcaaa taggtggcag acactggatt
     5101 ttgcaaagcc cgtcatgtgc gcgtctcaaa aagtcccggt cattgcttat gtccacggtg
     5161 gatggtggct gggtgggagc aaaacaggcg caatctattc catgccggcc aggcttgcat
     5221 accagcttgg ttttgctgtt gtctcgatcg agtacaggct cgcctcgtcc acaaaccacc
     5281 acccaaacct gataaatgac tgcaagctgg caatcagata tctgagggca aatgctgaca
     5341 tgcttggcat tgacccaaac aggattggca tctggggcgg ttcggctggt ggccaccttg
     5401 tttcactgat gggaacagca ggagatgacg atgggctgga gggtccggga tttgagggct
     5461 attcgagcag gccaaatgct gttgtcgacc agtgcggcat tgaagatctt acaaaacctt
     5521 gggacaatac ctcaagaatg gttttcggca ttttcctcgg gtgcgcatac aatgtttgca
     5581 cagacaaggc gcaggaggcc tcccctgtgt accaggccag ccctgatgac ccaccaatca
     5641 tgatcatgca tggtgaccat gacaatgtcg tttcatacac gcaggctgaa gagtttgcca
     5701 gagccttgaa agcaaatggt aataacgggt cttttatcag gatagtcaat ggaaaccacg
     5761 gttttggatc gtacaacggc cgtgaccttg tgtcaccaaa ctggactgtg caaaatcgtc
     5821 tcgtaatttc ccatctggca agatttattg agccggggct gatgtgcgac ctgaacatgg
     5881 acggaagggt tgaccagagg gatgcagatg agctttggat tcatcttggc gagtcaggtt
     5941 ttggcccgaa tgctgaaccg acagtggaat catggaaccc tcttgcagat attcatcctg
     6001 atggaaaaat cgattacaag gacatccaga gcttttgtgg ccaaattgca aaacctgtaa
     6061 cagtcgagaa aaaggtcatc ctgccaaaaa acagccagaa gatatacgaa tttgacatcg
     6121 aattatcaaa caatggcccg cttggcgttg gcaacataac aattaccgac tctttcccga
     6181 aggagcttgt ttttgtgtct tcttcccctc aaggggccct caagggcaac aagatcatca
     6241 tcgaaaactt gtccctgcca gcaaattcaa aatttacagc aagaattctc ttcaaattgt
     6301 cagatggcta caaacagcca gaagagggcg ccattttatc aaacagcgtc tccatttcct
     6361 cgcccgatat ttttaacata gaagatcagg tgacttttgc actgcccggg ataagaatca
     6421 gaaagacctc aaaatctttc aatgtcgcag agggtggatt ggcagttttt caggtgaccg
     6481 tcaccaatcc aagcgcccgc agactcacag gggttgaggt cacagacagt ttcccgcgag
     6541 aactggcttt tatatcatca agaccctccg gtgtagcctc caataaatca gtgaaatttg
     6601 aggtcgggga ccttggcccc ggtgaatcca gaattttcat gctcaatttc agacttccca
     6661 agctcgcctc aacaggcgag ggccaaacca tcacaaatac tgcctctgca tcttcaaatg
     6721 aattgcaccc tgttttttca agcgccagca ttttctgccc aaagaaggag cagggtggag
     6781 gagagctgaa tattgatatt gtctggaaag ggcccgattc aaaggggaga atcgctgtcg
     6841 gagaagagat cgagatgaca atgcaggtaa caggtggcag tgccccttat gatatctcga
     6901 ttgattttgg cgacaggaca agaaaattgt tctcgcttga tggggaaaga gcagtggaga
     6961 ttgcgcactc ttacagcgaa ccaggcgaat atgctgtgaa gatttcagtt tcggacgctt
     7021 atgggcgctc cagggttgtt cagcgaaaga taattgtcaa ataatttgat tgcattgagt
     7081 aattagattt tttcatcaat cctggatttt tatgcgaaac ccgtagatat cccagtccag
     7141 gccattgtct tctgcccaga caatgttgtc cccgaaaatt ctagcccagc atcttttgat
     7201 tgg
//
LOCUS       DAOSRI010000057         7181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9714_length_7181_cov_4.133736, whole genome shotgun sequence.
ACCESSION   DAOSRI010000057 DAOSRI010000000
VERSION     DAOSRI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9714_length_7181_cov_4.133736"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK717_02635"
     CDS             complement(<1..591)
                     /locus_tag="PK717_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR46592.1"
                     /translation="MKTAIVGKIIPLVPTMPEEFDGYVIVEENQIIDVGIGQPDGYFD
                     KVVDGPKQVVIPGLTNTHTHAAMSMFRGVADDLQLQTWLEKEIFPREDKLTDEIVYWA
                     TKLAIAELLLGGVTCFADMYFFMDEVAKAVSETGIRACLSKGLVSSSGEKEIEVGRAF
                     CQRWQGQGKGRIHTMLGPHAIYTCPPAYMEKVVRVAS"
     gene            complement(588..2147)
                     /locus_tag="PK717_02640"
     CDS             complement(588..2147)
                     /locus_tag="PK717_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR46593.1"
                     /translation="MKTVTSAQMREIDEQTSARFGVPSMLLMENAGASLCDIAMSEFS
                     TKSPVIVCGKGGNGGDGFVCARHLAMEGIQTKVFLLCKSEELTGDQAINFGILAKGFS
                     GFIELHVGETDQNLGKLSQAVKKADLIIDCLLGTGLLEAPKGAIAQAIELVNSVNLPV
                     LSCDIPSGVDGSTGKVPGLAVQADVTCTFGLPKIGHLLYPGASMVGKLLLTNVGFPKV
                     LLDDPYIEANVLTAQEASWLLPERTQSGHKGSFGKILIVGGSAEFPGAPILSGLGSLR
                     GGSGLTTVIVPESIYQSVAGRYPEMIVKGAPSDEYGAFSEHAAEHVLKAAFDASCIVI
                     GPGLSRAPGLSHFIGEVLGKIEIPVIVDADALNVLSKSPGSLLLRAEHGWPTILTPHW
                     GEAGRFGTAASDVTTDPIATARRFSNRYGSITVLKSARTIIASPSGQYYINTTGNSAL
                     SKGGSGDVLSGFIASLVSQGLSPFDASVLGCHLFGFAGEMTSKEHTEYGTMSTDVANS
                     IPKAIQEVLSQ"
     gene            2284..2733
                     /locus_tag="PK717_02645"
     CDS             2284..2733
                     /locus_tag="PK717_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOR46594.1"
                     /translation="MIWFTWQMWLIVALIFGIIEIATVNFYTIWLAIAAVITAILSVF
                     GLGIPGQILAFSLISLLLVLLSEAIVRKVIFGGHQEIKTNVDALKGQEGIVTFKIDNR
                     RGEGIVKIRGTDWSARSDDDESIPEGTIVIIQRIEGVKVIVRSKETK"
     gene            2880..3803
                     /locus_tag="PK717_02650"
     CDS             2880..3803
                     /locus_tag="PK717_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOR46595.1"
                     /translation="MTTESVIGYIILAFVIIFVLSTIIRSVRMVHQSKVMIVERLGRY
                     SRTLRPGLHVLIPFIDSVRAEVDLREQVLDYPPQSVITKDNVSAEIDTVIYYYITDPV
                     KAIYEIIDLNQAILKLSMTTIRNIIGDLDLDRLLTSRTQVNTELRAIIDEATDPWGVK
                     VNRIELKSIEPPSEIVDAMTKQMKAERDKRAVILESEGIKQSRILEAEGLKQSKILEA
                     EGQKQADILKAEGEAKAWETLWTAKSGSIKMYLDAIKQSGIDEKVLAVKYLESLGEIA
                     NGQANKVFIPYEATGMLSSLGAIKELFTKEK"
     gene            3869..4747
                     /gene="rapZ"
                     /locus_tag="PK717_02655"
     CDS             3869..4747
                     /gene="rapZ"
                     /locus_tag="PK717_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOR46596.1"
                     /translation="MNRTLIIVCGLSGAGKSRSLAAFEDIGYFTVENLPLELLSKFVS
                     LGIDAKDNMEHMAVTLRLPEEENYIDTLKEQIDTISASGVDVGVLYLEANTDTLIRRY
                     QETRRKHPLLNSDTNGSIFDAIQRERLLLRSLKKIATQIIDTSALTPQDLKVRISKIY
                     TIKSGKANLTITFLSFGFRYGVPSEADLVMDVRFINNPYYDESLRELDGTNVKIANFV
                     LSDESAQNFLLEFTNLLKFLLPQYLAKEGKSHLTVAFGCTGGKHRSVVIASEMANRLK
                     QEGYDVTVVHRDKDKQ"
     gene            4792..6027
                     /gene="yvcK"
                     /locus_tag="PK717_02660"
     CDS             4792..6027
                     /gene="yvcK"
                     /locus_tag="PK717_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOR46597.1"
                     /translation="MKRWFLLTLLAGMIISFGILTLDWASGSQRIMGWIGELFARITD
                     NTNLPSWFLPVIGWLMIGVGTAIAVSSLIQSIISMLRIVGQPSTKITKSSGPKVVVLG
                     GGTGIHPVLLALKSIGTSVTAVVTVADSGGSTGKLRQEFSMLAPGDIRQCLLALSDQP
                     LFDKIIRYRFTGQDSSLAGHSMGNLVIAALTDTEGDFTEAVFRLSKLMATKGTVIPFT
                     IDDVSLCAKYEDGTVVSGEANIPKINKPIKNVFFNPPYAKPYVRVIEAIAEADFVIVG
                     PGSLYTSIMPTLLLAPIVDTMINSKAKKIFMANVMTESGETNDYTILDHLEAIKKHTN
                     ADIFDMVISSNTPLPEEVLEKYSQSNASPLYASPEDMALIENLLTEDLATIDDGLARH
                     SVQKLSRVLSKIIFKGRKK"
     gene            6030..>7181
                     /gene="rpoN"
                     /locus_tag="PK717_02665"
     CDS             6030..>7181
                     /gene="rpoN"
                     /locus_tag="PK717_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOR46598.1"
                     /translation="MPSQSVRVSGTIKLKTNLRGVLKLHEALKILSMPAADLRETIQG
                     YLASNPILEEEPYKPDPEAEIRDRIEYKTRATDAEGLFSEEQIKSHEKTLYDILMEEP
                     QLSFDDEEKATIKEFLIGNIDDDGYLLCSIEEVADQLEVPEGTVEEVLNCIQEAAPPG
                     VGARNPQEALVLQIKHDLDDEEDRKIAISLVMEHYQNLKRRKIGEVAQNLNVSTEKIG
                     ELWDRIAKYSLSPGANYAPKADYITPEIQVRALGDQLQVLPLRDGIPNIVINQEYAQI
                     LKDNHEDDETSNYLKDKMQEAKWLIRAIDQRKKNMMRIVTEVSKVQGEFFKGPTTEIR
                     PLTLKDIADKLKLSPSTISRAINSKYVLTAKGTYPLKHFFTGGFSNGKTM"
BASE COUNT         2191 a         1650 c         1559 g         1781 t
ORIGIN      
        1 agaagcaacc ctaacaactt tttccatgta tgcaggaggg caggtgtaga ttgcgtgggg
       61 gccaagcatc gtatgtatcc tgcctttccc ctggccttgc catctttgac aaaatgccct
      121 gcctacttct atttcttttt ctccggagga gctgaccagt ccctttgaaa gacaagccct
      181 tattccagtc tccgagacgg ctttggccac ttcatccata aagaagtaca tatcggcaaa
      241 acaggttacg ccacccaaaa gaagctctgc tatggcaagt tttgttgccc aataaacgat
      301 ctcatcggtc agcttgtctt ctctgggaaa tatttctttt tcaagccagg tctggagctg
      361 gagatcgtct gccactcccc taaacattga catggccgca tgggtatgag tgtttgtaag
      421 cccaggaata accacctgtt ttggtccatc tacaactttg tcaaaatagc catccggctg
      481 tcctatgccg acatctatta tctggttttc ctctacgatc acatatccat caaactcttc
      541 aggcattgtt gggacgagcg gtatgatttt ccccactatg gcggttttca ttggctaagt
      601 acctcctgga tcgctttcgg gatcgaattg gcaacatccg ttgacatggt tccatactcg
      661 gtgtgctctt ttgaggtcat ctcaccagca aacccaaaaa gatggcaacc caaaacactg
      721 gcatcgaaag gagaaagacc ctgcgaaaca agggaagcta taaaacctga aagaacatcg
      781 ccagagccac cctttgacaa ggccgagttg ccagttgtat ttatgtaata ctggccagat
      841 ggggacgcaa ttattgtcct ggcagatttg agcactgtaa tagaaccata tctatttgaa
      901 aatcttctgg cagtagcaat cgggtcagtt gtaacatcac ttgcagcagt gccaaatctt
      961 ccggcctcgc cccaatgtgg cgtcaagatt gttggccagc catgctccgc ccttaaaaga
     1021 agtgatcctg gagattttga cagcacattc aatgcatccg catcaactat tacagggatt
     1081 tctatcttcc caagcacttc cccaatgaaa tgggaaaggc ctggggccct tgaaaggcct
     1141 ggaccaataa ctatgcaact cgcatcgaaa gctgctttta ggacatgctc tgcggcatgt
     1201 tcggaaaatg cgccatactc atcggaaggc gcccctttta cgatcatttc cggatatctt
     1261 ccagcaacgc tttggtatat agattcaggc acaataacag ttgtcaggcc agagccacca
     1321 cgtaatgagc caagccctga aagaatcggt gcccctggga attctgcaga ccccccaaca
     1381 atcaggattt tgccaaaaga ccccttatgg cctgactgtg ttctttcagg caagagccac
     1441 gatgcctctt gcgctgtcaa aacattggcc tctatgtacg gatcatcgag tagaactttt
     1501 ggaaatccaa catttgtaag caaaagcttt cccaccattg aagcacccgg ataaagcagg
     1561 tggccaatct ttggcaagcc aaaagtgcat gtaacatcgg cctgtacagc aagacccggt
     1621 actttaccgg ttgaaccatc gacaccgctt ggtatatcgc aggagaggac tggaagattg
     1681 acagaattta caagttcgat tgcctgggca atcgctcctt ttggagcttc caaaagcccg
     1741 gttcccaaca ggcaatctat tattaaatca gcttttttaa cagcttgtga caattttcca
     1801 aggttttgat cagtttcgcc aacatggagt tctataaaac cagaaaatcc cttcgccaga
     1861 atcccgaaat tgattgcctg gtcacctgtc aattcttcgg atttgcagag tagaaaaacc
     1921 tttgtttgga taccctccat ggccaggtgt cttgcgcaaa caaaaccatc accaccattg
     1981 ccaccttttc cgcaaacaat cacaggactt tttgttgaaa attcgctcat ggcaatatcg
     2041 caaagagaag ccccggcatt ttccataaga agcatggatg gaacgccaaa ccttgcggat
     2101 gtttgttcgt caatctctct catctgggct gaagttacgg ttttcattga ttcctccaag
     2161 tccattctat gtaaatggag tctaaaggca accttgaaaa gagagatttg gaacctagaa
     2221 tctaggaaaa gatttgtaaa aacgaattta tcatgttggt gtcaaagggc ttaaggaggt
     2281 ttaatgatct ggtttacatg gcagatgtgg ttgattgtgg cgcttatttt tggaatcatc
     2341 gagatagcaa cggtgaattt ttatacgata tggctggcta tcgctgctgt aataactgca
     2401 attctgtcag tttttggtct tggcattcca gggcaaatac tcgcattttc tttaatatca
     2461 ctccttttag tacttctttc tgaggcaata gtaaggaagg tcatttttgg gggacaccag
     2521 gagataaaaa caaatgtcga tgcacttaaa ggccaggaag gaatagtaac tttcaagatt
     2581 gacaatcgaa ggggtgaggg tattgtaaaa ataaggggaa cggattggtc ggcaaggtca
     2641 gacgatgacg agtcaatacc tgaaggaacc attgtaataa tacaaaggat tgagggtgta
     2701 aaagtcatcg taagatcaaa ggaaacaaaa tgaaaaaatt tttctggata atcacattac
     2761 tttcggcaat ctttctaggt ttttggtttg gttctaatat ggccttcgac aaagccatat
     2821 atgacgatga agattagaaa aaatcatttt ttaaatataa attggaggga attgtacaaa
     2881 tgaccacaga gagcgtaatt ggttacatta ttttagcctt cgtaattatt tttgtactgt
     2941 caacaatcat cagatcggta agaatggtcc accaatcaaa agtgatgata gttgaaagac
     3001 tcgggcgtta ttcaaggacc ctcaggcctg ggctccacgt tcttatccca ttcattgact
     3061 cggtaagagc agaggttgac ctcagggaac aggtgctgga ttacccaccg caatcagtca
     3121 tcacaaaaga caacgtctca gcagagatcg atactgtaat ttactactac attacagacc
     3181 cggtaaaggc catatatgaa ataatcgacc tcaaccaggc catactgaaa ctctcaatga
     3241 caacgataag aaacatcatc ggtgaccttg acctcgacag gttgcttacc tctagaactc
     3301 aggttaatac agaacttagg gcaatcatcg atgaagcaac cgatccatgg ggtgtaaagg
     3361 tcaacaggat agagctgaaa tccattgagc caccatcgga aatcgttgac gccatgacca
     3421 aacagatgaa ggccgagagg gataaaaggg ctgtaatcct agaatctgaa ggcatcaagc
     3481 aatccaggat acttgaagca gagggcctca agcagtccaa aatactcgaa gcagaagggc
     3541 agaagcaggc agacatcctc aaggcagagg gtgaggcaaa agcttgggaa accctgtgga
     3601 cagcaaaatc gggctcgata aagatgtatc ttgacgcaat caagcaatcg ggtattgatg
     3661 aaaaagtcct cgcggtaaaa tacctcgaat cacttggcga aatagccaat ggacaagcaa
     3721 acaaggtctt cattccctat gaggccactg gaatgctctc ttcgctgggc gcaataaaag
     3781 aactgttcac taaagaaaaa taaccatctt tttgtggcat aagacttgca ggacgggggc
     3841 caccatgtta acataagccc ctgtcctgat gaatagaact ttgataatag tctgtggctt
     3901 atcaggcgca ggcaaatcac gctctttagc agcctttgag gatattggtt atttcactgt
     3961 cgagaacctt ccactcgagc ttctttcaaa atttgtcagc cttggcattg atgccaagga
     4021 caatatggaa cacatggcag taacgctccg gctcccggaa gaagaaaact acatcgatac
     4081 actcaaagag caaatagaca ccatttctgc aagtggcgta gatgttggtg tgctctatct
     4141 tgaagcaaat actgacacac tcatccgcag atatcaggaa accaggcgca agcatccact
     4201 cttaaactcg gacaccaatg gaagcatttt tgatgcgatt caaagggaaa gactcttgct
     4261 tagatcgctc aaaaagatcg ctacacaaat aattgacacc tcagcactca ccccacagga
     4321 cttgaaggtc agaatatcaa agatatacac aataaaaagt ggcaaagcca atctcacgat
     4381 aacttttctg tcctttggat tcaggtatgg tgtgccatct gaggctgatc ttgtcatgga
     4441 tgtccgtttt atcaacaatc cctattacga tgaatcttta agggaactcg atggaacaaa
     4501 tgtgaaaata gcaaactttg ttctctcgga cgaatcggca caaaattttc ttttggaatt
     4561 tacaaacctt ctcaaattcc tcctcccgca atatcttgca aaagagggca aaagccatct
     4621 caccgttgca ttcggctgca caggcggaaa gcatcgctct gtggtaatag cctctgaaat
     4681 ggccaacagg ttaaagcagg aaggctatga tgtaaccgtg gttcataggg acaaagacaa
     4741 gcaatgacaa ccaaagagct cgttaggatt gtctatcgcc ctggtgcagg cgtgaagagg
     4801 tggttcctcc tgacacttct tgctggaatg atcatttcct ttgggatact gactctcgat
     4861 tgggcctcgg gttcccaaag gataatgggc tggatcggcg agctgtttgc cagaattacc
     4921 gataatacca atctcccatc ctggttcctg cctgttattg ggtggttgat gataggtgtc
     4981 ggcactgcta ttgccgtatc ttccctgatc cagtcgataa tctcgatgct caggatagtt
     5041 ggccagcctt ccacaaaaat taccaaaagc agtggcccca aggtcgttgt tctcggtggt
     5101 ggcacaggca tccatccagt tcttctggca ctcaaatcaa tcggcacctc ggtcacagcc
     5161 gttgtcacag tcgccgattc tggtggttca accggaaaat tgaggcagga attttcaatg
     5221 ctagcgcccg gagatataag gcagtgtctc cttgcattgt ccgatcagcc attgttcgac
     5281 aaaatcataa gatacagatt tacaggacag gactcttccc tcgccggaca ttcaatgggc
     5341 aaccttgtca ttgcagcact cactgacacc gaaggcgatt ttacagaagc agtgttcagg
     5401 ctctcgaaac tcatggcaac aaaaggcacg gtcattcctt tcacgattga tgacgtttca
     5461 ctttgcgcaa agtatgaaga tggcactgtt gtttccggtg aagccaacat tccaaaaatc
     5521 aacaaaccaa taaaaaatgt cttctttaac ccaccctatg ccaagccata cgtcagggtc
     5581 atagaggcca tcgctgaagc agattttgtt attgttggcc caggaagtct ctatacatca
     5641 ataatgccaa cactccttct tgcaccaatt gttgacacaa tgataaactc aaaagcaaag
     5701 aagattttta tggcaaatgt gatgactgaa tcaggcgaaa caaatgatta cacaattctt
     5761 gatcatcttg aagccatcaa aaaacataca aatgccgaca tttttgatat ggtaatttcc
     5821 agcaacactc cactcccaga agaagtattg gaaaaatata gccaatcaaa cgcatctccc
     5881 ttatatgcaa gcccagaaga catggccctg atagagaacc ttctgaccga agatcttgca
     5941 accatcgatg atggccttgc aagacattca gtgcagaaac tttcaagggt tttgtcaaaa
     6001 atcatcttta aaggaagaaa gaagtagcaa tgcccagtca atcagttcgt gtatcaggca
     6061 ctatcaaact caagacaaac ctcaggggag tcctcaaact ccatgaagca ttaaaaattc
     6121 tttcgatgcc agccgccgac ctcagggaaa caatccaggg ttatctggca tcaaacccta
     6181 ttttggaaga agaaccatac aagccagatc cggaggcaga aatccgcgat cgcatcgagt
     6241 acaagaccag ggcaacagat gccgaaggac ttttttcaga agaacagata aaatctcatg
     6301 aaaaaaccct gtatgacatt ttaatggaag aaccccaact gtcatttgat gatgaggaaa
     6361 aagcaaccat aaaagaattc ctcattggaa acattgacga tgacggatac ctgctctgct
     6421 cgatagaaga agttgcagac cagcttgaag ttccggaagg gactgtagag gaagttctaa
     6481 attgcattca ggaagctgct ccgcctggtg tcggtgccag aaacccccaa gaagcattgg
     6541 tgttgcaaat taagcatgat ttggatgatg aagaagacag aaaaattgca atatccctgg
     6601 tcatggaaca ctaccaaaac ctcaagagga gaaaaatagg cgaggtagcc cagaacctca
     6661 acgtatctac tgaaaaaatt ggcgaattgt gggacaggat tgcgaaatac tccctgtcac
     6721 caggagccaa ttacgcccca aaggcagact acataacacc tgaaatacaa gtcagggcac
     6781 tcggcgacca actccaggtg cttccactaa gggacggtat tccaaacata gtaataaacc
     6841 aggaatacgc ccaaatactg aaagataatc atgaagatga tgaaacctca aactatctta
     6901 aagacaaaat gcaagaggcc aaatggctta taagagccat tgaccagcgc aaaaaaaaca
     6961 tgatgagaat agtgaccgag gtatccaagg tccaggggga atttttcaag ggcccgacaa
     7021 ccgaaatcag gccgctcaca ctaaaagata tcgctgacaa gctaaaactt tccccatcaa
     7081 caatatcaag ggctatcaat tcaaagtatg tattaacagc aaagggcacc tacccgttaa
     7141 agcatttttt cacaggtggc ttcagcaatg gcaagaccat g
//
LOCUS       DAOSRI010000058         7164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9745_length_7164_cov_3.837530, whole genome shotgun sequence.
ACCESSION   DAOSRI010000058 DAOSRI010000000
VERSION     DAOSRI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9745_length_7164_cov_3.837530"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="PK717_02670"
     CDS             <1..1727
                     /locus_tag="PK717_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR46599.1"
                     /translation="IDALIANYDQYQKTIKQKPTKDGLIIHIKRLSPTLIGDLAAIRA
                     SSYESSSFGFVCLDNSFDGKALIGSSNSYKNSWYPRLVKLSLAFPPYIKWQNSVLPEI
                     NPYITTTKKPSKDGSSAIGSALGTDYMLETDKSGWEPPIMLVYASQSYERMDEDIGAI
                     EKSIGNWIWITLIVGVFCAILLGLSISSRIISVKRGIGNLALDLTKPVPHVSGELGDI
                     ADAANKLAIDLMKSKSRSERVLDSVNTGIMVVGSDMKIIQANPSSLKIAKKTKQEIIG
                     SDISAIGEPGEIAKSEVEKAIKSGSVWSSGSVKVRGSNETRYVNMRVVPVQMAQENEA
                     ILAIQDVTESVVNTLETERDASLARLGLFTMGIAHEVRNPLTSIKGFVQLLERKLKDR
                     DESRYLSPVMKEVERLEILITDLLESSRPGPIKRKEVDLSKLVNDVLAGQANKLTEAG
                     VRISKECRPGVIANVDEKRFHQVILNLILNAKDAMPEGGTLSLKTGESGEWFHIEVRD
                     TGMGISEQDAANIFAPFFTTKASGTGLGLSICDQIVKSHGGSISFTSDENGTTFIVKI
                     PKIIKMED"
     gene            1731..3005
                     /locus_tag="PK717_02675"
     CDS             1731..3005
                     /locus_tag="PK717_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOR46600.1"
                     /translation="MDYKVDISKIMLGLNIIIIGLYILLGNTGVVDFGYLAYFIKFWP
                     LIIITVGLDMILRNYNLGYIGSVIFSIFLVLAIIASTNGPAGTSVRKFLTVPENPTSW
                     SNYWGEWNINIFDFTPAETKSYSEKLDPASKAIVFDMSSPRIKNASIILKKSTAVSIK
                     YDIMPRSKINGEARLAMITDFDGSLTVKEPEKIDVDMKIKMEVQVPETTVCIFRGTQG
                     SIEIQDDWKGGLDFAGLTNYSIKTKNLDQNFEFKTVSGSINIGSAKNMRVETGSGNIR
                     MEGTTGELTATTISGLVVVNSFENGTIKTTSGDISAKNAGKIEIKTISGDVKVESVGS
                     MMSISTTSGDIMIGEFNSSSSSSKISSISGDVSVGLGKQASIKGECSSTSGDINLSGL
                     RRTESANGFMFGDGRGSLDISTTSGDISVLER"
     gene            3007..3666
                     /locus_tag="PK717_02680"
     CDS             3007..3666
                     /locus_tag="PK717_02680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR46601.1"
                     /translation="MKHKYNPPGTKTASAKPVKTISKTKKQSQKANSFRFWTILIGSI
                     ILVGSIVLAIALSNGQNGKATPTGNPVSVLPKLDPSKFSPKATDFTLKDTEGTTHKLS
                     DHGGRPVFLDFTASWCGWCKKQAPDMKKLFQKYGSTVQFFAIDVNEPLNVVKSYKDEE
                     SAPWPFLLDENGQVSAQFGVKGYPHYILLSPDGTINKIQSGYSESFFQDFSSAIEGII
                     R"
     gene            3701..4018
                     /gene="rnpB"
                     /locus_tag="PK717_02685"
     ncRNA           3701..4018
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK717_02685"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4011..4901
                     /gene="rsmH"
                     /locus_tag="PK717_02690"
     CDS             4011..4901
                     /gene="rsmH"
                     /locus_tag="PK717_02690"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR46602.1"
                     /translation="MGQGEYHHTPVMLPEAMAFLRPERGGVYFDCTCGEGGHSEAILK
                     ASPNSKTVCMDLDLNMLMVAKERFKEFEGRTWFVHTNFTDIDKAASLTNVTNSDGILM
                     DLGISMAHLKGEKRGITFMEDQPLDMRLDINRGEPAYSILSRIKFEELARAISEYGEE
                     RFAGKIARAIINTREKEPINTTGQLVKIISSVVPRSGRINPATRTFQALRILVNQELE
                     SLEEAIPKCLDLLGWDGTLVVISYHSLEDRIVKNAFKNAADKGFEILTKKPLCPSEEE
                     IEANPASRSAKMRAIRRKGG"
     gene            4894..5211
                     /locus_tag="PK717_02695"
     CDS             4894..5211
                     /locus_tag="PK717_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46603.1"
                     /translation="MAKKKKKNKKSRRVLLFIAIIFAVWWIWMYFSNHMQAVKLETEI
                     AKKIDLKEQLQRELADLNQKRKEMLSLGEIKEYATKNLGMVVSSGKRFFLPDPRILPK
                     DQK"
     gene            5208..6941
                     /locus_tag="PK717_02700"
     CDS             5208..6941
                     /locus_tag="PK717_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR46604.1"
                     /translation="MKQFRPNTFRLGFMLFLCVALFGTLFAKLFIVQFINANEFNQRA
                     KQQYRKPNEEIPATRGEIFDRNGIRLVTNSITYDLVCEPEKISNPDGMGEAISSVIGM
                     EPKIVQDRLKGIGGTKSYTIIKKNLDSKIADKIRSFGFKGIWLETRFVRSYPQQNLAS
                     PLIGFTGNDNHGKYGLEIDFDKELSGESGLRTYPMDSKGIAGRTYKVLKPVINGKSLY
                     LTIDNTIQFVVEEELERTCKEWNAKGGACVVMNPQTGEIIACVSYPTYNLNDALNSND
                     NQRRNRALNYVYEPGSVFKGIVTAAALELGKIDDNFTADCAGRTVFKGYRLFCNNRHG
                     HQEKLAEELKNSCNMAFMKIGQLIGKDLCRFVNDFGFGKKTGTGAHEESGLVKNSRSW
                     DDVTIATVSYGQGVSVTPLQLAQAYSALANGGVLMKPILVQQIYDPNKGTFERNAPQP
                     IRQVVSKKTADHVLRLLTEVVDMKEDGVHQAKISGYSVAGKTGTTPKTTASGDYQFSK
                     FDCSFCGIVPSDPLAPQKFVVLVAIDEPYKTTASGGRINPFGSTTAAPCFKRIAERIL
                     SDLRIAPKEIK"
     gene            6938..>7164
                     /locus_tag="PK717_02705"
     CDS             6938..>7164
                     /locus_tag="PK717_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOR46605.1"
                     /translation="MRARELFEKAGLEYGGPDFEVSEIVFDSRKAGKGKVFVALRGSH
                     TDGHKFIDKAIGAGSEVAVSEEAGINRIVVLV"
BASE COUNT         2268 a         1644 c         1692 g         1560 t
ORIGIN      
        1 aaatagatgc ccttatcgcc aattatgacc agtatcagaa gacaataaag caaaaaccaa
       61 caaaagatgg cctcataata cacatcaaaa ggctctcgcc aaccttaatt ggcgaccttg
      121 ccgcaataag ggccagctcc tatgagtcat cttcttttgg tttcgtttgt ctggacaact
      181 cttttgacgg gaaagcacta ataggcagct caaacagcta caaaaacagc tggtacccaa
      241 ggcttgtaaa actttcgctt gcattcccgc cctacataaa atggcaaaac tcggtactcc
      301 cagaaataaa tccatatata acaaccacaa aaaaaccttc caaggatggc tcctcagcca
      361 ttggttcagc actcgggaca gactatatgc tcgagactga taaaagtgga tgggaaccac
      421 caataatgct tgtctacgcc tcgcaatcat acgagcggat ggacgaagac ataggagcaa
      481 tcgagaaatc aatcggtaac tggatctgga taaccctgat tgttggagta ttttgtgcga
      541 tccttctcgg attgagcata tcctcaagga ttatctcagt caaaaggggc atcggcaatc
      601 ttgcgcttga cctcacaaaa ccagtcccac atgtttcagg cgagcttggc gacatagccg
      661 acgcagcaaa caaactggct atcgacctca tgaaatcaaa gagcaggtcg gagagggtcc
      721 tggattccgt aaacacaggt atcatggttg ttggctcaga catgaagata attcaggcaa
      781 acccatcatc attaaaaata gctaagaaaa caaaacagga aatcattgga tctgatatta
      841 gtgcaatagg ggaacctggt gaaattgcca aatcggaggt tgaaaaggca ataaaatcag
      901 gttctgtgtg gagttccggt tccgtaaaag taagaggatc aaacgagaca agatacgtca
      961 atatgagggt cgttccagtc cagatggcgc aagaaaacga ggccatcctt gcaattcagg
     1021 atgtaacaga aagcgtggtc aacacccttg aaacagaaag agacgcttcc cttgcaagac
     1081 tcggcctgtt cacaatgggt atcgcccatg aagtcagaaa ccccctcacc tcaataaaag
     1141 gctttgtcca gctgcttgag cgcaaactaa aggaccgtga cgaatcaagg tacctctctc
     1201 cggtaatgaa agaagtcgaa aggctcgaaa tcctcatcac tgatcttctt gaatcatcaa
     1261 gacccggacc gatcaaaagg aaagaggtcg atctcagcaa acttgtgaat gatgtcctgg
     1321 caggccaggc aaacaagctc accgaggctg gtgtaaggat ttcaaaagaa tgcaggcctg
     1381 gcgtcattgc caatgttgat gaaaagaggt tccaccaggt catcctgaac ctcattctaa
     1441 acgcaaagga tgcgatgcca gaaggtggca cactttccct caaaacaggt gaaagcgggg
     1501 aatggttcca cattgaagta agagacaccg gcatgggtat cagcgagcag gatgcagcaa
     1561 acatttttgc gccattcttc accacaaagg catctggcac cggcctggga ctttcgattt
     1621 gtgatcagat tgtcaaatcc cacggtggat caatttcttt cacatcagat gaaaatggga
     1681 ccacattcat tgtaaagata cccaaaataa taaaaatgga ggactaataa atggattaca
     1741 aagtggatat atcaaagata atgcttggtc tcaatatcat cattattggg ttgtacatac
     1801 ttttgggaaa tacaggagtg gtggattttg gctatctggc atattttatc aaattctggc
     1861 cgttgataat aatcacagtc ggtctggaca tgatccttcg caattacaac cttggctata
     1921 tcggttcagt gattttttcc atttttcttg ttctggcgat aattgcatcc accaacgggc
     1981 ctgccggcac atcggtaaga aaattcctta ccgtgccaga aaacccaacc tcctggtcaa
     2041 attattgggg ggaatggaac atcaatattt ttgacttcac accggccgag acaaaatctt
     2101 actcagagaa acttgatcct gcatcaaagg caatcgtatt cgacatgtct tcacccagga
     2161 taaaaaatgc ctccatcata ctcaagaaat caacggcagt ttcaataaaa tatgacatca
     2221 tgcccagatc aaagatcaat ggcgaagcaa gactcgccat gataactgac tttgacggga
     2281 gccttacagt caaggaacct gaaaaaatcg atgtagatat gaaaataaag atggaggttc
     2341 aagtcccgga aacaacagta tgcatcttca ggggcaccca gggttcaatc gaaatacagg
     2401 acgactggaa gggtggcctt gactttgctg gtctcacaaa ctattcgatc aagacaaaaa
     2461 accttgacca gaattttgaa ttcaagacgg tttctggctc cataaacata ggatcagcaa
     2521 aaaacatgag ggttgaaaca ggttccggaa atatcagaat ggaagggact actggagaac
     2581 tcacagcaac aacaatatca ggcttagtcg ttgttaattc attcgaaaac gggacaataa
     2641 aaaccacttc aggtgacata agtgcaaaaa atgccggaaa gatagaaata aaaacgatct
     2701 ctggggatgt aaaggtagaa tcggtgggca gcatgatgtc catctcaaca acatcaggcg
     2761 acataatgat cggggaattc aactcaagct ctagctcttc aaagatttct tctatatctg
     2821 gggatgtatc agtcgggctt ggaaaacagg catcgataaa gggtgagtgc tcatcaacaa
     2881 gtggcgacat caatctctca ggcttgagaa ggacagaatc tgcaaacgga ttcatgtttg
     2941 gcgatgggag aggttcgctt gacatttcaa caacatcagg cgacatttca gttctggaga
     3001 gataacgtga aacacaaata caacccgcca gggaccaaaa cagcatcagc aaaaccagtg
     3061 aaaactattt caaaaaccaa aaaacaatcg caaaaagcaa acagcttcag gttctggaca
     3121 atactcatcg ggtcaatcat cctggttggc agtatcgtct tggcaatcgc gctcagtaat
     3181 ggacaaaacg gaaaagcaac accaacaggc aatccggttt cagtgctccc aaagcttgat
     3241 ccatcaaaat tttcaccaaa agccaccgat ttcacgctca aggacaccga gggaacgaca
     3301 cacaaactat ccgatcatgg tggaagacca gtatttttgg acttcacagc atcgtggtgc
     3361 ggctggtgca aaaaacaggc cccggacatg aaaaaactgt tccagaaata tggaagcaca
     3421 gtccaatttt tcgctattga cgtcaacgag cctctgaatg tcgtcaaaag ctacaaagat
     3481 gaggaatctg ctccatggcc cttccttctc gatgagaatg gacaggtttc agcccagttt
     3541 ggcgtaaagg ggtatcctca ctacatactg ctttccccag atggaacaat caacaagata
     3601 caaagcggct acagcgaaag tttctttcag gatttctcca gcgcaattga aggaattatt
     3661 cgttgaaaaa ctcaggatgg gtggtattat gttgaatgtc tcaagcgggc aggcaatcgc
     3721 ggggtttagt gcctcgagga aagtccgggc tccgcagagc agggtgctgg gcgcaagccc
     3781 agtaagcgca agcttaagga aagtgcaaca gaaaatatac cgcgccccaa aagggcgtaa
     3841 gggtgaaatg gcggggcaag ggcccaccgg catccgggcg accggatggc catgtaaacc
     3901 ccacccggag caaggccaaa taggaggagg tcaagggctg ctcgtccgtc tcctcgggta
     3961 ggccgcccag agaaatgatt gcccacgaca gaacccggct tacaacccgc ttgggacagg
     4021 gagaatacca tcacaccccg gtcatgctcc ctgaagcaat ggctttcctt cgccccgaaa
     4081 gagggggagt atattttgat tgcacatgtg gtgaaggagg ccactcagag gccattctta
     4141 aggcatcacc caattcaaaa acagtctgca tggacctcga cctcaacatg ctcatggtgg
     4201 caaaagagag attcaaagag tttgagggtc gcacatggtt cgtccacaca aatttcaccg
     4261 atatagataa agcggcatct ctgactaacg ttacaaactc tgatggcatc ctcatggatc
     4321 ttggcatttc aatggcccat ctcaagggtg aaaagagagg catcacgttc atggaagacc
     4381 agccactgga tatgaggctt gatataaaca ggggtgagcc ggcatactcg atactctcga
     4441 ggatcaaatt cgaagagctg gccagggcca taagcgaata cggcgaggag aggttcgccg
     4501 gaaaaatagc gagggcaatt atcaacacca gggagaagga accaataaat acaacaggcc
     4561 agctggtcaa gataatcagc tccgttgtac caaggtccgg tagaatcaac cctgccacga
     4621 ggacttttca ggcgctcagg attttggtaa accaggagct ggaaagcctt gaagaggcaa
     4681 tcccaaaatg ccttgacctg cttggctggg acggaactct ggttgtgatc tcctaccact
     4741 ccctggagga caggatcgta aaaaacgcct tcaaaaatgc cgcggacaaa gggtttgaaa
     4801 tcctcacaaa gaaacctttg tgtccctccg aagaagaaat tgaagcgaac ccagcttcac
     4861 gctctgccaa gatgagggcc atcaggagga agggtggcta agaagaaaaa gaagaacaaa
     4921 aaaagcagaa gggtgctttt atttattgca ataattttcg cagtgtggtg gatatggatg
     4981 tatttctcga accatatgca ggcagtcaag ctggaaaccg aaatcgccaa aaaaattgac
     5041 ctcaaggaac agctccagag ggagctggct gacctgaacc agaagcgcaa ggaaatgctt
     5101 tctcttggcg aaataaagga atatgccaca aaaaacctgg gcatggttgt gtcttcagga
     5161 aaaaggttct tccttcctga cccgagaatc cttccgaagg accagaaatg aagcagttca
     5221 ggccaaacac cttcaggctt ggttttatgc tttttttgtg tgttgcactt tttggaacac
     5281 tttttgcaaa actttttatt gtccagttta taaatgcaaa cgaattcaac cagagggcca
     5341 aacagcaata tcgcaaaccc aacgaggaaa taccagccac aaggggcgaa atcttcgaca
     5401 ggaacggcat ccggctggtc acaaattcca taacatacga ccttgtctgt gagcctgaga
     5461 aaatatcgaa cccggacggt atgggcgagg caatatcaag tgtcatagga atggaaccaa
     5521 aaatagtcca ggatagactc aagggaattg gcggcaccaa aagctacacc ataataaaga
     5581 agaacctgga ctcgaaaatt gctgacaaga taagatcatt tgggttcaag ggaatatggc
     5641 ttgaaacaag atttgtaagg tcatatcccc aacaaaacct tgcatctcca ctaatcggat
     5701 ttacaggcaa tgataatcac ggaaaatacg gactcgaaat cgattttgac aaagaactct
     5761 cgggagagag cggtctcagg acatacccga tggactccaa aggcatagca ggaaggacct
     5821 ataaagtctt aaaaccagtc atcaacggaa aatcgctcta tctgaccata gacaacacaa
     5881 tccagttcgt tgttgaagag gaattggagc gtacctgcaa agagtggaac gcaaagggtg
     5941 gcgcatgtgt cgtaatgaat ccccaaacag gcgagattat tgcttgcgta tcatatccca
     6001 catacaatct gaatgatgcg ctcaactcaa acgacaatca gagaagaaac cgcgcactca
     6061 actatgtcta cgagccagga tcggtattca agggcatagt cactgcggcg gcccttgaac
     6121 tgggcaagat agatgacaat ttcacggccg attgcgccgg tcgaacagtt ttcaagggtt
     6181 atcggctctt ctgtaacaac aggcatggcc atcaggaaaa acttgcagaa gagctcaaaa
     6241 attcctgcaa catggccttc atgaagatag gccagctcat tggcaaagat ctgtgcaggt
     6301 tcgtcaatga ttttggcttt gggaaaaaaa ctggtaccgg ggctcacgag gagtccggcc
     6361 tcgtcaagaa ttcccgctcc tgggacgacg tcacgatagc aactgtctca tacggacagg
     6421 gagtcagtgt caccccgctc cagctggcgc aggcatactc tgcactggca aatggcggag
     6481 tcctcatgaa accgatactg gtccagcaaa tatatgaccc caacaaaggc actttcgaga
     6541 ggaacgcccc gcaaccaatc aggcaggtcg tatcaaaaaa gactgcggac cacgtcctca
     6601 gacttctcac cgaagtcgtt gacatgaaag aggacggcgt ccaccaggcc aaaatctccg
     6661 gatattctgt ggcaggcaaa acgggtacca ccccaaaaac aacggcaagc ggagactacc
     6721 agttcagcaa gtttgattgc agcttttgtg gcatagtacc ttctgatcca ctcgcaccac
     6781 aaaaatttgt tgtccttgtc gcaattgacg aaccgtataa aacaactgca agtggcggaa
     6841 ggataaaccc ttttggctcg acaactgccg ctccatgttt caagagaata gcagaacgta
     6901 tactttcaga cctcaggatc gcacccaagg agataaaatg agagcaaggg aactttttga
     6961 aaaagctggc ctcgaatatg gtgggccaga ttttgaagtt tcagagattg tttttgattc
     7021 aaggaaagct ggcaagggaa aagtttttgt cgccctcagg ggctcccata ctgacggcca
     7081 caaatttatc gacaaagcaa tcggggccgg ttctgaggtt gcagtttccg aagaggccgg
     7141 cataaacagg attgtagttc tagt
//
LOCUS       DAOSRI010000059         7162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9750_length_7162_cov_3.856620, whole genome shotgun sequence.
ACCESSION   DAOSRI010000059 DAOSRI010000000
VERSION     DAOSRI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9750_length_7162_cov_3.856620"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="PK717_02710"
     CDS             complement(<1..1752)
                     /locus_tag="PK717_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46606.1"
                     /translation="MNAEQWRLLGRLESRFEENQQDVSRAIDLAEFLKGLPEGRGALL
                     NFLAQIQQKFPGSFKIAEISYRSYKEMGDKEKAAQFEKILLDIKPEDLQDLRRKLLIF
                     KEKDQPDKASEEIESYEKDKGRTVETLWMAGDLYATTDNQEKLLKVIEDLKVLKDQPN
                     AEIAIAALTMATKGDQKENWEEAQKLITQSLKKDPSNMLAYRVFFLGSAKFAPSAESM
                     SKIVDASIKANITEPFIFVEIMRLHLEYTIPLHNKEVFEKLIALLSETEKPVINTIHA
                     LCCYFDNIGLARNVMRMWLDPQGDGPDFSASLAQINNDLISSIESRVGISLSDNKDKL
                     DELKKFLKENSISCMQRLIAQAPGNPDAYYQMGVLLQGFKDERAYQYYMKALEIRPDH
                     VYAQFQIAKINDDEQNDIEAYERYRKIVNMVVVDPSIAVDAMMRASEIAIKYGWIEEA
                     EEMLEKARTILPSDPRVVTMLAKIYLKEAKIIGSDYALEQAMRYFMQAISINPRNMEA
                     SYYLGHVFYINHQYLDAIRQFNDTAQKFQTVSILCDFWISRSYHQLYKNLLFSSEDFL
                     TSAIKYAEYLRVMGEKVP"
     gene            complement(1749..2525)
                     /locus_tag="PK717_02715"
     CDS             complement(1749..2525)
                     /locus_tag="PK717_02715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR46607.1"
                     /translation="MDKKSREILEKSKKVKSKAELEKIVKQEKQARAQKDKLLRKKIF
                     TIGGISLAAVAVVVLVAILISSCSSNGNTTKEEEKKTKAVVETTKGSFTIQLDYDNAL
                     SVVNNFVKNAEAGLYDNSYFHEILPGMKIQGGYPETDKRPQGVGAYDVIEYVSNEVTH
                     AKYSVCAPAMEEKDSKGNVTKSGLSKTQFYMLLSDQKSLDGKDTVFGKIIDGANVVEE
                     LAKLETVEQGEKNKYNIDPTEKSRPKNIEQAKIIKVKITQ"
     gene            complement(2497..3351)
                     /locus_tag="PK717_02720"
     CDS             complement(2497..3351)
                     /locus_tag="PK717_02720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR46608.1"
                     /translation="MASLPVKLLLGINKLFPKGQKHPLDLEKENKGSYAAWEYDLAKK
                     TIGYFPSDFAVEELVKDKDILDDCCGAGGKAVYLLDLGAKSVTGVDIGQEFVAMADDF
                     AAIRRAKNCKFVAGDAMSLPFPDKSFDLVFAFDAFEHVKDPVLMLKEARRVLRPGGKV
                     VMSFTTWGRPGGHHLTDAIRFPFAHLFFSEKTLLGAYRTLVDDERYIFRAGSLDSKKI
                     EYVNHMSFKKAKKIISASGLRVLLYEGVPYPGIFKFLAKMGLGEYFYRVVTTILEEPD
                     GQEKQRNP"
     gene            complement(3366..4088)
                     /gene="recO"
                     /locus_tag="PK717_02725"
     CDS             complement(3366..4088)
                     /gene="recO"
                     /locus_tag="PK717_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOR46609.1"
                     /translation="MPYIKSEAIILAKKTFGDADKQLVLVTDSDTVIEAKIRKAKDTK
                     TRWACVTEPVSIVDCELYERSQRFTITSLSLQKYYSSFGTSFEKSAVSEFISEAVCFF
                     VPQNIEESGIYELLLNSFECLDTSKNPLYSMGLFLSGFFALLGYPIDTQYCSLCSESI
                     DGGAFFDYGSTSFLCGECAKPGMPSVPGFAIGALRRMQYADWKNWEMDEKLVKGVCTL
                     LARALERKFEKNLHSTLHVIGL"
     gene            4156..4449
                     /locus_tag="PK717_02730"
     CDS             4156..4449
                     /locus_tag="PK717_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46610.1"
                     /translation="MDKFSKKLTDDEIRLKFIRIPKGKEKLFKKDDFKTSFNEGEFIA
                     YIYPVESKSMGPNMKTYEYQLKFRDPQPPIFIFNETLEFTTFDSGETWLISFA"
     gene            complement(4510..5580)
                     /locus_tag="PK717_02735"
     CDS             complement(4510..5580)
                     /locus_tag="PK717_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46611.1"
                     /translation="MKKILALMAIVCLFASCSGGENGDNLPFLSLSWAWSDLAEPLST
                     TVRGNGKYVACCGLNGVEVALLNEADGVPVWHIEQDEKPTNETNFPPVFAGSRLIVYC
                     SDSLKAFELVSGYEVWKFQTEQTNLGPASFFEPSYDYNQIYAGTASGFLYCINAGSGI
                     GNWKQKSRYEGFGETVNMYDKVLVRTLGSRIEAYNKSTGKLVWKNERWVLPDGRFTID
                     DKNLYLAMPGPFVSAIRHNSGELVWTSGKLSTSKAPLKFHPLVCQGKVVVLEGKKATL
                     FDSATGKEETSFDLPEEPETFTTEGSSLYLTKGKMLFCVDCTGKVKGTFEEVQGDSLL
                     GVSFSQGHVITWSPRTIYGLRK"
     gene            5663..>7162
                     /locus_tag="PK717_02740"
     CDS             5663..>7162
                     /locus_tag="PK717_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46612.1"
                     /translation="MRKIELVDLTLRDGQQSLVATRMTTEQALSAFPDLLDAGFKELE
                     LWGGATIDAPLRFLNENPWGRLDEFYRLAKGRANIRALIRGQNLFAYSPYPDNLVIAF
                     CKAAIRSGVSTMRAFDALNDKRNVMISLIASKAFGGKAECCISYTTSPIHTTEKFVQL
                     AADYASEGADIIAIKDMAGLLNPKDAAIIIPAIKKEISVPLTVHSHSTVGYGETTALV
                     GLMFGADRIDVAVGPFAGGSSHPPVELVAVMAERLGIDHRLNHEAIQRAQKKLFEVRK
                     ALAKFDSSANNLPKPIPNPLPQTDIDKIDKAIELVRKGDFDEARRTIVDLMTSYGYPK
                     PDEAQLDAQVPGGMLSNLRNQLKEVNQLQLLPQILEEVARVRADSGYPPLVTPTSQIV
                     GSQAAFNVQTGQRYKIVSREFKDMVRGRYGRPGPISEEFLKMVTGSTERYTQRSGHYV
                     DDVPLTSENGFNPPPFINNHRDLLLYYMLPGPTKDFFEKQDSKAKSPEQP"
BASE COUNT         1884 a         1720 c         1497 g         2061 t
ORIGIN      
        1 aggtactttc tcccccatca ccctgaggta ttcggcatac tttattgcag aagtcaggaa
       61 gtcttccgag gagaaaagaa ggtttttgta gagctggtgg tatgacctgg atatccagaa
      121 atcgcaaagt attgaaacag tctggaattt ctgggccgtg tcattgaact gtctgatcgc
      181 atcaagatac tggtggttta tatagaagac gtggccaaga taataagagg cttccatgtt
      241 ccttgggtta atagatatgg cctgcatgaa atacctcatg gcttgctcga gggcataatc
      301 agaaccaatt atctttgctt ctttgaggta aatctttgca agcattgtaa cgaccctggg
      361 gtcagatggc aaaatagtcc ttgccttctc cagcatttcc tctgcttctt ctatccaccc
      421 atattttatc gctatctctg aagccctcat catcgcatct acagcaatgc ttgggtctac
      481 aaccaccatg ttcacaattt ttctgtatct ctcgtaggct tcgatgtcgt tttgctcatc
      541 atcatttatc tttgcgatct ggaactgggc ataaacatga tccggcctta tttctaaggc
      601 cttcatgtaa tactggtacg ccctctcgtc tttgaatccc tgcaatagca cgcccatctg
      661 gtaatacgca tccgggtttc cgggcgcctg cgctatgagc ctctgcatgc aacttattga
      721 attttccttc aagaatttct ttagctcgtc aagtttgtct ttgttgtccg aaagcgaaat
      781 tccaaccctg ctttcaattg acgatatgag gtcattattt atctgcgcaa gtgaagcgct
      841 gaagtcaggg ccgtcacctt gtggatcaag ccacattctc atgacgtttc tcgcaagacc
      901 aatattgtca aaatagcaac agagagcatg tattgtattt attaccggtt tctctgtctc
      961 tgacagcaaa gcgatgagtt tttcgaaaac ctccttgttg tggagaggga tcgtatattc
     1021 gaggtgaagg cgcattattt caacaaaaat gaagggttca gtgatattgg cctttattga
     1081 cgcatcaaca atcttgctca tggattctgc tgatggcgca aatttagccg acccaaggaa
     1141 aaataccctg taggctagca tgttggaggg gtcttttttg agggattggg ttatgagctt
     1201 ttgtgcttcc tcccaatttt ctttttggtc gccctttgtt gccatagtca gggctgctat
     1261 ggctatttca gcatttggct ggtcttttaa aaccttcaaa tcttctatta ctttcaggag
     1321 tttttcctgg ttgtcagtgg ttgcataaag atcgcctgcc atccagagtg tttcgactgt
     1381 ccggcccttg tctttctcat agctttcaat ctcttcactg gctttgtcag gctggtcttt
     1441 ttctttaaaa ataagcagtt tgcgtctcag gtcttgcagg tcttccggtt tgatgtccaa
     1501 aagtattttt tcaaattgtg cagctttttc cttgtctccc atctctttgt aggaacgata
     1561 tgatatttct gcaattttaa acgagccagg aaacttttgc tgtatctggg caaggaaatt
     1621 caaaagggcc cccctgcctt cgggaagccc tttaaggaac tcggccaggt caatcgcccg
     1681 gctgacatcc tgctgatttt cttcaaaacg gctctctagt ctgccaagca atctccactg
     1741 ttctgcgttc attgtgttat tttcaccttt attattttgg cctgttcaat attttttggc
     1801 cttgatttct cagttggatc aatattgtat ttgttctttt caccctgctc cactgtctcg
     1861 agctttgcca actcctccac gacatttgca ccatcaataa ttttaccaaa aactgtgtct
     1921 ttgccatcca gagatttctg gtcagaaagg agcatataga actgggtttt tgacaaacca
     1981 gattttgtca cattgccctt gctgtctttc tcttccatgg caggagcgca cacggaatac
     2041 tttgcatgag taacttcatt gcttacatat tctattacat cataggcgcc aacaccctgt
     2101 ggtctcttgt cagtttcagg gtatccgccc tgtatcttca tgcccggcag gatttcgtgg
     2161 aaataggaat tatcataaag ccctgcttcg gcattcttta caaaattgtt cacaacagaa
     2221 agcgcattgt catagtccag ctgtattgta aaggaccctt ttgtggtttc tacaacagcc
     2281 tttgtctttt tctcttcttc ttttgtggtg ttgccatttg aagagcatga agaaatgagt
     2341 attgcaacca aaacaacgac ggccactgcc gcaagagaaa tgccaccaat tgtaaaaatt
     2401 ttctttctga gcaacttgtc tttttgggct cttgcctgtt tttcctgctt tacgatcttc
     2461 tccagttcag ctttcgattt gacttttttc gatttttcaa ggatttctct gcttttcttg
     2521 tccatctggc tcctccagta ttgtagtaac tactctgtaa aaatactcac caagtcccat
     2581 ttttgcaagg aacttgaaaa tccccggata tggcacccct tcatacagga gtacccttaa
     2641 ccctgaggca gaaattatct ttttcgcttt cttaaaagac atgtggttca catattcaat
     2701 cttttttgag tccagcgaac cggccctgaa aatatacctt tcatcatcta ccaatgtcct
     2761 gtatgcacca aggagtgtct tttcgctaaa gaacaggtgt gcgaagggga accttattgc
     2821 atcggtaagg tggtggccac cgggtctgcc ccaggttgtg aatgacatta caacttttcc
     2881 accgggtctg agcacccttc tggcttcttt taacatcaat accggatctt tcacatgttc
     2941 gaaagcatca aacgcaaaaa ccaggtcaaa agatttgtcc gggaaaggca atgacattgc
     3001 gtcccctgcc acaaacttgc aatttttggc tctccttatt gctgcaaagt catctgccat
     3061 tgctacaaat tcctggccaa tatcaacacc ggttacggat tttgcgccaa gatcgaggag
     3121 atagacagct ttccctccgg cgccacaaca atcatcaagg atgtctttgt ccttaacaag
     3181 ctcctcgact gcaaaatctg atggaaaata tccgattgtc ttctttgcca gatcgtactc
     3241 ccaggccgcg tatgaaccct tgttttcttt ttcgaggtcc aaggggtgtt tctgtccttt
     3301 tggaaacagc ttgttgatgc caagtagaag ctttacgggg aggcttgcca ttctctggat
     3361 tctaactaca gtccgattac atgtaaagta gaatgaaggt ttttttcaaa ctttctttcc
     3421 agtgctcttg caagaagtgt gcacacaccc ttgaccagtt tttcgtccat ctcccagttt
     3481 ttccagtctg catattgcat tcttctcaat gctcctatcg caaacccggg aacgcttggc
     3541 atgccaggtt ttgcgcactc accacataga aaggatgttg acccatagtc aaaaaaagcc
     3601 cctccatcta tactctcgga gcaaagcgag caatactgtg tgtcaatcgg gtatcccaaa
     3661 agcgcaaaaa aacctgatag aaaaaggccc attgaataaa gcggattctt tgatgtgtct
     3721 aggcactcaa agctgttcag aagcaattca tatatgccac tttcttctat attctggggc
     3781 acaaaaaagc aaacggcctc tgaaataaac tcgcttacag cagatttttc aaatgatgta
     3841 ccaaaacttg aatagtattt ttggagcgac agcgatgtta tcgtgaatct ctgcgatctt
     3901 tcgtaaagct cgcagtcaac aatggaaacg ggctctgtta cacaggccca ccttgttttt
     3961 gtgtcctttg ccttacgtat ttttgcctca ataacggtgt cagaatcagt tacaagcaca
     4021 agctgcttgt ctgcatcgcc aaaggtcttc ttggcaagta ttatcgcctc ggacttgata
     4081 tacggcacgt ttcgatgata aaccctgttt aaaaaatgga caagagatgt ataatggttt
     4141 ttaaggaggc ctcaaatgga caagttttca aaaaaactca ccgatgacga gataaggctg
     4201 aaatttatca ggatcccaaa gggcaaagaa aaactcttta aaaaagacga ctttaaaacc
     4261 agtttcaatg agggtgaatt tattgcctat atttacccgg tagaatccaa aagcatgggc
     4321 ccaaacatga aaacctacga ataccagctc aaattcaggg acccgcaacc gccgattttt
     4381 atattcaacg aaacgctgga gttcacaacc tttgattcag gggaaacgtg gctcataagc
     4441 tttgcgtgac aattgttgac gatttgtttc caaattgttt atatctactg tgagtatgtg
     4501 gagaactatt tattttctaa gtccataaat ggttcttggg ctccaagtta ttacatgtcc
     4561 ttgtgaaaaa ctcaccccga gcaagctgtc accttgcact tcttcgaatg ttccctttac
     4621 tttaccggtg caatcaacac aaaacagcat tttccctttt gttagataaa gggagctgcc
     4681 ctcggttgtg aaagtttccg gctcctccgg caagtcaaac gatgtctctt ccttgccagt
     4741 ggcagaatca aaaagggtcg cctttttacc ctctaaaacc accaccttgc cctggcagac
     4801 aagcgggtgg aactttaggg gtgccttgct tgtggatagt ttccctgaag tccacacaag
     4861 ctcgccagaa ttgtgtctta tggcggaaac aaagggtcct ggcattgcaa gatagaggtt
     4921 cttgtcatcg attgtaaatc ttccatcagg aagcacccat ctctcgtttt tccagacgag
     4981 cttgcctgtg ctcttgttgt aggcttcaat ccttgagcca agcgtcctga caagaacctt
     5041 gtcatacatg ttgactgttt caccaaaacc ctcgtacctg ctcttttgct tccagttgcc
     5101 tatgcccgaa ccagcattga tgcaatacaa aaaacctgag gcagttccgg cataaatctg
     5161 gttgtaatcg tatgatggct caaaaaaact cgccgggcca agattggttt gttcagtttg
     5221 gaacttccac acctcatatc ctgaaaccag ctcaaacgcc ttgagggagt cagaacaata
     5281 aacgattagt ctggatcctg cgaatactgg agggaaattg gtctcattcg ttggtttttc
     5341 gtcttgctca atatgccaga ccggtacacc atcggcctca ttcagaaggg ccacttcgac
     5401 accattgaga ccgcagcaag caacatattt tccatttccc ctcacggtcg tggagagagg
     5461 ttctgcaagg tcgctccaag cccaggagag cgagaggaat ggcaaattgt caccattttc
     5521 cccacccgaa catgacgcaa aaaggcatac gatggccatt aatgcaagga tttttttcat
     5581 ttatacgcaa tattatttta cttaacgtct tatgtaaacc ctaattgtat ttagcctgcc
     5641 caaccaatat gatgaactgg atatgagaaa aatcgaactt gtagacctta cactaagaga
     5701 tggacagcag agccttgttg caacaaggat gacaaccgaa caggcgcttt cagcctttcc
     5761 agatctgctt gatgcaggtt tcaaggagct ggagctgtgg ggaggggcca caatcgatgc
     5821 tccactgagg tttctgaatg aaaatccatg gggaagactt gacgaattct acagactggc
     5881 caaaggaaga gcaaatataa gagccctgat aaggggccaa aacctttttg catattctcc
     5941 ttatccggac aaccttgtca tagctttctg caaggctgca ataagatctg gcgtttcgac
     6001 catgagagca tttgatgccc tcaatgacaa acgaaatgtc atgatttcac tcatcgcttc
     6061 aaaagcgttt ggtggaaaag ctgaatgctg catcagctat acaacctccc cgatacacac
     6121 cacagaaaag ttcgtccagc ttgccgccga ctacgcatca gaaggcgctg acatcatagc
     6181 aatcaaagac atggctggtc tcctcaatcc caaggacgcc gcaataataa taccagcaat
     6241 caaaaaagag atttccgttc cattgacggt ccacagccat tcaacggttg gctatggcga
     6301 aacaaccgca ctggttggtt tgatgtttgg tgccgacagg atagacgtcg cggttggtcc
     6361 gtttgctgga ggttcgagcc atccaccagt ggagcttgtt gcagtaatgg cagagagact
     6421 cggcattgac cacaggctca accacgaagc gatccagagg gcccagaaga agctctttga
     6481 ggtgagaaaa gccctggcaa agtttgattc cagtgccaac aatctcccga agccaattcc
     6541 caatccgctc ccacagacag acatagacaa aattgacaaa gcaatcgaac tagtcagaaa
     6601 aggcgatttc gacgaggcaa ggagaactat tgttgacctg atgacctcct atggctaccc
     6661 aaaaccggac gaggcccagc ttgacgcaca ggtgccaggc ggaatgctct cgaaccttcg
     6721 caaccagctc aaggaagtta accaactcca gctcctgcct caaattctcg aggaagttgc
     6781 cagagtaagg gcggattcag gataccctcc gcttgtcact ccaacatccc aaattgtcgg
     6841 ctcccaggca gctttcaatg tacaaacagg tcagcgttac aagatcgtct cacgggaatt
     6901 caaggacatg gttaggggaa ggtatggcag accaggcccg atatctgaag aattcctgaa
     6961 aatggtgacc ggaagcactg aaagatatac tcagcgttca ggccattatg tggatgacgt
     7021 cccgctcact tccgaaaatg gcttcaatcc accaccattc atcaataatc atagggatct
     7081 tctgctttat tacatgctcc cagggcctac aaaagacttt ttcgagaaac aagactcaaa
     7141 agcaaaatca ccggaacagc ct
//
LOCUS       DAOSRI010000060         7045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9933_length_7045_cov_3.692132, whole genome shotgun sequence.
ACCESSION   DAOSRI010000060 DAOSRI010000000
VERSION     DAOSRI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9933_length_7045_cov_3.692132"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="PK717_02745"
     CDS             complement(<1..2552)
                     /locus_tag="PK717_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR46613.1"
                     /translation="MAKKTTSRIVTCLSSIDFDALAASLCISKLYPGVIPVKISGVER
                     PVQMFLGIYKDFIRLEDFEEYDPDSIRELFIVDTRPSKKVWDFVASFKNLQRCIIFDH
                     HTASRGHANCRLVQKEVGSTTTMLLPLLKLRKVYISPQEATVMLTGVYEDTANLISPT
                     TTPQDLEVAAYLLSLGASLQVVRRFLSTALDQRGKDILKQFLDNIEVVPMGGILIAIS
                     CVKYKDYVENLAFITHKLFDLVEADAIFTACSMTGNTYFVGRSLDDRLVDSAKVLSHF
                     GGGGHKTASAAKIKGELDPKLAFYRIRKVCEEEIVPPPTITSIMSSPVKTVGPEVPVS
                     EALKQVQRFGHGGLPVVNEDRLIGIITRRDLDRVPDEYQHRPVKRYMTTNVVTIDAGA
                     SIVEARRLMMKKSLGRLPVVSSGRVVGIVTRSDVLKASFWHRGQDIGPFPPPTFIDKT
                     QTRELQQKVPASIQEKIDVFVRIANELGHNLYLVGGAVRDSIMDKEPTDLDILVEGDA
                     VNLAKSAAEELGGKITTHERFGTARMLLDGIQYDFATARTEYYTEPGAYPNVIQSSIR
                     EDLMRRDFTINALALSLNGPNKDKILDYCGGFDDIATKKVRVLHNLSFVEDPTRILRA
                     AYYSKILNFTLEKETKRLAVDAIKTGLLATAKNERTAQEFEKILRHEKGSEMLYLLEQ
                     MDGLTVIFGQLPDGLHQLLLKADRLAAITARKGFKPDLRVLRCLVLGMAIDKGALREC
                     LSRIRMSGKFIDTVEVSKEGSLVLSQAIGRGDRLEIFRSLKNLNDSAVVFACMGGKLP
                     ENLKNLDLVGKIRNIRLEITGEDLISNGLKKTKNFKAIIDQVAADTRKVLAD"
     gene            2641..3930
                     /gene="aspS"
                     /locus_tag="PK717_02750"
     CDS             2641..3930
                     /gene="aspS"
                     /locus_tag="PK717_02750"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOR46614.1"
                     /translation="MRILAKDAVNHIGEEITLEGWIHNLRELPEFSFIVLRDRTGTVQ
                     GILENPDFEVPQIGNEYVIRMKGNCVKSEIAKAGFEIKVTGIEVLSKVVDELPTQINK
                     KNPVEHLDRLLDYRYFSLRHPKLAAVFKVQSCISRGFREYLSSQEFTEIHTPKIVASG
                     TEGGANLFKVDYFGRPAYLAQSPQFYKQMMVGVFERVFEVAPVFRAEPHNTSRHVNEY
                     TSLDFEMGYIKDENDIMDMEEAVLQYVVGLVNETCQAELELLGAKVSIPSKIPRMRMT
                     EVHEILEKKYKIKYPQGFDIDPEGERAICEYVQKETGSEFVFIDGYPIEARPMYTMPD
                     LEHPGDTRGFDLIYRGMEITTGGQRIHGFDMLVDNIRKFKCNPDDFTFYTDVFKYGMP
                     PHGGLAIGLERITCQMLGLKNIREGTLFPRDLDRLTP"
     gene            3930..5192
                     /gene="miaB"
                     /locus_tag="PK717_02755"
     CDS             3930..5192
                     /gene="miaB"
                     /locus_tag="PK717_02755"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOR46615.1"
                     /translation="MSSFCFLTIGCQMNVSDSEFYSSILLESGFVEAPEEEADIIFIN
                     TCAVREGSVHKLDSKLGELYKRKRQTGLPKIGVVGCVPAIDLKSFKKRHKQVDFVLGT
                     YHDRESMQGALEEALGFELEHGISPRASSVSAFVPITTGCDSFCTYCIVPYTRGRLVS
                     REIEEILSEAKSYIESGAKEIVLLGQNVNAYGLDKGKKDAFVDLVRRVSELPGVLRVR
                     FITSHPKDMDKETLLKLREIKNLAWFFHLPIQAGSDEVLSRMNRRYTVEDYRKLVENV
                     RKIFPGAGVTTDVIVGFPGETEEQFEQTMALFRELRFEQAYMAMYSQRPKTPAANFSG
                     QVDREELHRRVSTIVEQQKRIAEQVGADYVGRVLSVLVENVEDGHSFGLSETGRLVKI
                     KGQFEKGTIVKVKISKPGMRSLEGEPVQ"
     gene            5189..6418
                     /locus_tag="PK717_02760"
     CDS             5189..6418
                     /locus_tag="PK717_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOR46616.1"
                     /translation="MNPTTYLSKCESELSGHFARIDSMADRRLMGIVEAYRKVGLTNS
                     DLCGSTGYGLGDSSRDKLEEVYSIVFGGESALVRPQIASGTHALACVLFGLSRPGDRI
                     VSITGTPYETLQEVIGIRQSVGSLSEFGVSYAQTDWRADGPKDFDLLAPPTKIVMIQR
                     SPGYKTGRPVTIDNIGEWVEVIRKAGSEAAIMVDNCYCEFCSEKEPCHAGADLSVGSL
                     IKNPGGGLAFTGGYVVGKSDLISRIATRVTAPGIGAEVGATWGFMLDAFRGLFAAPSL
                     SSSALKGALLWAKCFAGLGFEVNPKHDEEHADVVVSIKLDSRELFMAFGKAIQEVSPI
                     ASGATPVPFDQGGYDAPVMLAGGGFISGGTSDLSCDGLDKPPYWVYVQGGANMNHIRL
                     GILNVLSSFERMGFFGA"
     gene            6408..6803
                     /locus_tag="PK717_02765"
     CDS             6408..6803
                     /locus_tag="PK717_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR46617.1"
                     /translation="MAHRLTVRTPKRNAFVDITRLVQDFVSVSGFIDCTVSIFCPHTT
                     CAVTINENSDPDVVSDALNFFSAMVPKNSGFSHSEGNSDAHIKTMLFGNSVSVFVEGG
                     KLLLGTWQAIYLAEFDGPRERQIWLKITK"
     gene            6782..7027
                     /locus_tag="PK717_02770"
     CDS             6782..7027
                     /locus_tag="PK717_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46618.1"
                     /translation="MAENNKVVLGVLSACSPQYVFVGKGKKVLSSLEINYSNGGNKTL
                     PAVASCLEQAKMTIQDVDFFLALSGPGSLTGIRNWIR"
BASE COUNT         1761 a         1608 c         1819 g         1857 t
ORIGIN      
        1 tggtcggcca gcaccttgcg ggtgtcagca gcgacctggt cgatgatggc cttgaagttt
       61 tttgtcttct ttagcccgtt tgaaatgagg tcctctccgg ttatttcaag cctgatattg
      121 cgtattttac ccaccaggtc gaggtttttc aagttttcag gcagtttgcc acccatgcag
      181 gcaaagacaa ccgcagaatc attgaggttt tttagcgacc tgaatatctc caggcgatca
      241 cccctgccaa ttgcctggga gagcaccaga gaaccttcct tggatacttc aactgtgtca
      301 atgaatttgc cagacattct tattcttgag aggcactccc tcagtgcgcc cttgtcaatt
      361 gccatcccga gaacaaggca cctcaggacc ctcaaatcgg gcttgaagcc ttttcttgcg
      421 gtaattgctg ctagcctgtc agccttgaga aggagctggt ggaggccatc cggcaactgc
      481 ccaaagatga ccgtgagtcc gtccatctgc tccagaaggt agagcatttc ggaacccttt
      541 tcgtgcctta gtatcttttc aaactcctgt gccgtcctct cattcttggc tgtggcaagg
      601 aggccggtct ttatggcgtc taccgcgaga cgctttgtct ctttttccag cgtaaagttc
      661 aatatttttg aatagtatgc ggccctcaga atcctggttg ggtcttctac aaaagagaga
      721 ttatgcaaaa ctcttacttt cttcgttgct atgtcgtcga accctccgca ataatcgagg
      781 attttgtcct tgtttgggcc attgagcgag agggcgaggg cgtttattgt aaaatctctt
      841 ctcatcaggt cttccctgat tgagctctgg atcacattcg gatacgcacc aggttcggtg
      901 tagtattctg tccttgccgt ggcaaaatcg tattgtatcc catccagcag cattcttgcc
      961 gtgccgaacc tctcatgggt cgttatttta ccacccaatt cctctgcagc tgatttagca
     1021 agattgactg catcaccctc tacaaggatg tccagatcgg taggctcttt gtccatgatg
     1081 ctgtccctta cggcgccacc aacgagatag aggttgtgtc ccagttcgtt ggctatcctc
     1141 acaaatacgt ctattttttc ctgaatgctg gctggcacct tttgctggag ctctctagtt
     1201 tgggttttgt cgatgaatgt tggtggaggg aatggcccga tgtcttgccc cctgtgccag
     1261 aaagaggcct tgaggacgtc cgaacgggtt acaatgccaa ctaccctacc acttgagaca
     1321 actggcaatc ttccaagcga cttcttcatc atgagtcttc tggcttcaac gatggacgca
     1381 cctgcatcta ttgtaacaac gtttgttgtc atgtaacgct tgaccggcct gtgctggtat
     1441 tcgtcgggga ctctgtccag gtcgcgcctt gtgatgatcc ctatcaacct gtcttcattg
     1501 acgacaggca gcccgccatg tccaaatctt tgcacctgtt ttagtgcttc acttaccggt
     1561 acctctgggc caacggtttt gaccggtgag ctcatgatcg aagtgattgt tggcggtggg
     1621 acaatttctt cttcgcagac ttttctgatt ctgtagaatg caagttttgg gtcgagctca
     1681 ccttttattt ttgcggccga tgcagtttta tggccacccc cgccaaaatg gctaagcacc
     1741 tttgcggagt caaccagcct gtcatcaagc gacctgccca caaaatatgt gtttccggtc
     1801 atggagcagg ccgtaaatat tgcgtctgcc tcaaccaggt caaagagctt gtgggttatg
     1861 aaagcgagat tttcaacata atccttgtat ttgacgcatg aaatggcgat aaggatgccg
     1921 cccatcggga caacctcaat attgtccagg aattgcttga gtatgtcttt tcctcgctgg
     1981 tccagcgcag tagagagaaa tctcctgacg acctggaggc ttgcaccaag cgacagcagg
     2041 tatgccgcga cctcaaggtc ctgcggggtt gttgtcggcg atatgaggtt ggcggtgtcc
     2101 tcatagacac ctgtcagcat gacggtcgct tcctggggtg aaatgtaaac ttttctaagt
     2161 ttgagaaggg gcaggagcat cgtagtggtt gatccgacct ctttttggac gagcctgcaa
     2221 ttggcgtgtc cccttgaggc cgtgtgatgg tcaaaaataa tacaacgctg gagatttttg
     2281 aaacttgcca caaaatccca gacttttttt gaaggcctgg tgtcaacaat aaaaagttcc
     2341 cttattgaat cagggtcgta ctcctcaaag tcttcgagcc ttatgaagtc cttgtatata
     2401 cccagaaaca tctggactgg tctctctact ccggaaattt ttacaggaat cacccctggg
     2461 tacagctttg atatgcaaag ggaggccgcc agagcatcga agtctatgga cgacaggcat
     2521 gtgacgattc tggatgttgt ttttttggcc atcaatgcga gattacactc acggttccta
     2581 gctgtcaatt acttgaacaa actttaccat cagatatatt gtttgcggaa aggaccggac
     2641 atgagaatac ttgcaaaaga cgcagtcaac catattggcg aagaaataac gcttgaaggc
     2701 tggatacaca atctcaggga gctccccgaa ttcagcttca ttgttttgag agacagaacg
     2761 ggaactgtac aggggatcct tgaaaatccc gactttgagg tcccgcaaat tggaaacgaa
     2821 tatgtgataa ggatgaaggg caattgcgta aaatccgaaa tcgcaaaggc aggttttgag
     2881 ataaaagtaa ccggaattga agtcctctca aaagttgttg atgaattgcc cacccagatc
     2941 aacaagaaaa atccggttga gcatctggac aggctccttg attacaggta tttttcactc
     3001 agacacccaa agcttgccgc agttttcaaa gtacaatcct gcatatcaag aggattcagg
     3061 gagtaccttt cttcgcaaga atttaccgaa atacacacac cgaaaattgt tgcctctggg
     3121 accgagggtg gcgcaaacct cttcaaggtg gactattttg gccgtccggc atatctggcc
     3181 cagtcgccgc aattctacaa acagatgatg gttggcgtgt ttgagagggt ttttgaggtt
     3241 gccccagtct tcagggcaga accacacaac accagcaggc acgtcaacga atacacgtcc
     3301 cttgatttcg agatgggcta catcaaggac gagaatgaca taatggacat ggaagaggca
     3361 gttctccagt atgttgtagg gctggtcaat gaaacatgcc aggccgaact ggaactgctt
     3421 ggcgccaaag tctcaatccc ctccaaaatt ccgaggatga ggatgacaga ggtacatgaa
     3481 atccttgaaa agaaatacaa aataaagtat ccgcaaggat ttgacatcga tcctgagggc
     3541 gaaagggcca tttgcgagta tgtccagaaa gaaactggct cggagtttgt tttcattgat
     3601 ggctacccga tcgaggcaag gccaatgtat acaatgcctg acctggagca ccctggtgac
     3661 acaagagggt ttgacctcat ctacaggggc atggaaataa caactggtgg ccagaggata
     3721 cacgggtttg acatgctggt ggacaacatc cgcaaattca agtgcaatcc cgatgacttt
     3781 accttctata ccgacgtgtt caaatatggc atgccgccgc atggagggtt ggcaatcgga
     3841 ctggagagga taacctgcca gatgctcgga ctgaaaaaca taagggaggg gacgctcttc
     3901 ccaagggacc tggacaggct cacaccttaa tgtcatcttt ctgttttttg acaatcggtt
     3961 gtcaaatgaa tgtttctgat tcagagttct attcgagcat cctgcttgag tcagggttcg
     4021 ttgaggcccc tgaggaagaa gcggacatta ttttcataaa cacctgtgca gtcagggaag
     4081 gctcagttca caaactggat tcgaaacttg gcgaactcta caagaggaaa aggcagacag
     4141 gccttccaaa aataggggtt gttgggtgtg ttccggctat agacctgaag agtttcaaaa
     4201 aaaggcacaa acaggttgat tttgtcctgg gcacatacca tgacagggaa tcaatgcagg
     4261 gtgctctgga agaagccttg ggatttgagc tcgagcatgg catttctccg agggcgtctt
     4321 cagtttcggc gtttgtacct ataacaactg gttgtgacag cttctgtacc tattgcattg
     4381 tcccgtacac gaggggcagg cttgtttcca gagagattga agaaatcctt tctgaggcaa
     4441 aatcatatat tgaatccggg gccaaagaaa ttgtccttct tggccagaat gtgaatgcct
     4501 atggtttgga caaaggcaaa aaggacgctt ttgtcgatct ggtacgtaga gtgtcagaac
     4561 ttccaggcgt tctcagggtc agattcataa cttcccatcc aaaagacatg gataaagaga
     4621 cccttttgaa actgagggaa ataaaaaacc ttgcctggtt tttccatctt cccatacagg
     4681 ctggctcgga tgaagtcctc tcaaggatga acaggcgcta tactgtagag gactacagga
     4741 agcttgttga aaatgtgaga aaaatattcc caggggccgg cgtgacaacc gatgttattg
     4801 tcggtttccc tggcgaaact gaagagcaat tcgagcagac gatggcactt ttcagggagc
     4861 tccgttttga acaggcatac atggcgatgt actcgcaaag gccaaagacc cctgcggcca
     4921 atttctccgg ccaggttgac agggaggaat tgcaccgaag ggtaagcacc attgtggagc
     4981 agcaaaagag gattgccgaa caggttggcg cggactatgt tggcagggtc cttagcgtgc
     5041 tggtcgaaaa tgttgaagat gggcattctt ttgggctctc ggagaccggc aggcttgtca
     5101 agataaaggg ccagtttgaa aaagggacaa ttgtaaaggt aaaaataagc aagcctggta
     5161 tgaggagtct ggagggtgaa cctgtccaat gaacccgaca acttatttgt caaaatgcga
     5221 gagcgagctt tctggccatt ttgcccgaat tgacagcatg gctgacagaa ggctcatggg
     5281 aattgttgaa gcctacagaa aggtcggcct cacaaattct gatctgtgtg gttcaactgg
     5341 ctatgggctt ggggattcct cgagagacaa gctcgaagag gtgtactcga ttgtttttgg
     5401 tggagagtct gcactcgtga ggccgcagat tgccagcggc acccatgccc tcgcgtgcgt
     5461 cctgtttggc ctgtcgaggc caggggacag gatcgtttcg ataacgggca ccccttatga
     5521 gactttgcag gaagtgattg gcatcaggca atctgttggt tcgctttcag aatttggtgt
     5581 ttcctacgct caaacggact ggcgggctga tgggccaaaa gattttgatt tgctggcccc
     5641 accgacaaaa atcgtcatga tacagcgttc acccggatac aaaacggggc ggccggtaac
     5701 gatagacaac attggggaat gggtggaagt catcagaaaa gccggcagtg aagcggccat
     5761 aatggtggac aactgctatt gtgaattttg ttccgagaag gaaccatgcc atgctggtgc
     5821 cgatctttcg gttggatcac tgataaaaaa tccgggtggc gggttggcct ttacgggtgg
     5881 gtatgttgtt ggtaaaagtg acctcatttc cagaattgcc acaagggtca cggcaccagg
     5941 catcggggct gaggtcgggg caacgtgggg tttcatgctg gatgctttca gggggctttt
     6001 tgctgcccct tcactttcat cctctgcgct caaaggggcg cttttgtggg ccaaatgttt
     6061 tgccggcctt ggttttgagg tgaacccaaa gcatgatgag gagcacgcag atgttgtggt
     6121 ttcaataaag cttgattcaa gagaactctt catggctttt ggaaaggcca ttcaggaggt
     6181 atcgccaatt gcttcaggtg caacacccgt cccatttgac cagggtgggt atgacgcacc
     6241 agtgatgctg gccgggggag gtttcatatc cggaggtaca tcagatcttt cctgtgacgg
     6301 ccttgataaa cccccttact gggtttacgt tcagggagga gccaacatga accacataag
     6361 gctgggaatt ttgaatgttc tttcttcttt tgaaaggatg gggttttttg gcgcataggc
     6421 tcacggtcag gacaccaaag agaaacgcct ttgtcgatat aacaaggctt gtccaggatt
     6481 ttgtctcggt ttcaggtttc attgactgta cagtcagtat tttttgtccc cacacaacat
     6541 gtgctgtgac catcaatgag aactctgatc cagacgttgt ttctgacgca ctcaacttct
     6601 tctctgcaat ggtgccaaaa aacagcggtt tttcccattc cgaaggcaac tctgatgcac
     6661 acataaaaac aatgcttttt ggcaattcag tcagcgtttt tgttgaaggt ggcaagcttt
     6721 tattgggcac atggcaggca atctatcttg ccgagtttga cgggccaagg gaaaggcaaa
     6781 tatggctgaa aataacaaag tagtactcgg cgtgctctct gcctgttccc cgcaatatgt
     6841 tttcgtaggg aagggcaaaa aagtcctttc gagtctggaa ataaattatt ccaacggtgg
     6901 caataaaacc ttgcctgccg ttgcctcgtg ccttgagcag gcaaaaatga caatacaaga
     6961 tgtcgatttt tttcttgcgc tctcagggcc aggaagtttg actggaataa gaaattggat
     7021 acggtaatag acgtctttcc atcag
//
LOCUS       DAOSRI010000061         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_9959_length_7027_cov_4.334050, whole genome shotgun sequence.
ACCESSION   DAOSRI010000061 DAOSRI010000000
VERSION     DAOSRI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9959_length_7027_cov_4.334050"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1050
                     /locus_tag="PK717_02775"
     CDS             265..1050
                     /locus_tag="PK717_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOR46619.1"
                     /translation="MSTTLLLGIAGANFGRTARERFTLPVYLSPSADSNIAENMSEQI
                     KLLPNVKDVKVLTPDDLLKKEGESLHLSPEQIIQSISYNPLPYSLVVVPENPQNIDAL
                     AKRIKQFTMVEDVDYESDVINKFVNLFDLFIWIALGLAVLSILVSSIVIGSTISLSIA
                     SRIEEIEVMSFVGASPGTILGPFIIEGVFYGLIGGGISAAGIYFGWKYLQTIFQTNLP
                     WFNLSIEQTVFYSIVAGTVVFGFLTGFITSWLSSRNTLRRIRA"
     gene            1047..2207
                     /locus_tag="PK717_02780"
     CDS             1047..2207
                     /locus_tag="PK717_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOR46620.1"
                     /translation="MKKIVPALLACLIAFSSTPLIKAGNLQDELRSINNRIITNKRRL
                     SETKKYQDVLGIKEKSKLDEQATVKGLLDEYETKTASTQQEIVETVKRIEEARKNMET
                     KGRELDKKRDEVGKLLTFVHKFSQNRFIDYVFSAESFSELTGRARFTNKILNGTVKAI
                     TDLHLESSRIETARQNLEKDKQRLAELKTEYKTLVERYGGLKKKNDQILNDIKNQKQW
                     TKDELIRIQRQLDNDQAQVKIILKKIAEEEERRKRAAYKGAYIWPIPVNGPIGPFGMR
                     KHPIFGDVRMHTGIDIDAKMGAPIMAAGDGFVGFVGQLGGYGNLVILSHGNGITTFYA
                     HMSRFGCSKGQQVKKGQVIGYIGSTGWSTGPHLHFEYRINDKPMNPLSYLPR"
     gene            2212..3381
                     /locus_tag="PK717_02785"
     CDS             2212..3381
                     /locus_tag="PK717_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR46621.1"
                     /translation="MRENRYTFWVTISTFLIIVFGLGIFCGVLVGSRAAKSTQTKIDI
                     NNMPLLSKTVQVVMDNYYRADMVTEENLAKAMLFGLDPYSTFFNKDEYKLFQDSTVGN
                     YVGIGVTIGNDQSKRHVIVLQVFDKSPALRAGVEEGWEIVKINGSKLKSTDIDYVSSL
                     LKGKENTSINLTFQHDGLFVDKTIAREQITINTVSSKNIDKYTYIRLSNFMPQSFEEM
                     KNAINNFELSGSKGLIFDLRRNPGGFVKSCIDISNLFLEPGTLAFTKDQAGRTEEIKT
                     SGIRFEKPLVVLVDHFTASASEVLTGALQDRGVATVIGTRTFGKGLIQNIIPVPPFGV
                     IKLTIQSYLTPNRNEVDVVGIKPDIEVSEGVDNFYPLDPKEDPFIQKAIEILKEK"
     gene            3385..5976
                     /locus_tag="PK717_02790"
     CDS             3385..5976
                     /locus_tag="PK717_02790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOR46622.1"
                     /translation="MNWHSIPLDETLKTIDATLQGLSSKDSKNRLEKYGPNEISAKER
                     SSVLQMIVEQFVSPLILILLAACIFSIIQGEWVDAIAIITVLVANATLGIIQQYKSEG
                     ALEALKKMSAPKAHVFRDGSIHEIPSSEVVPGDLVILSAGDLVPADLRLVETINLACN
                     EAILTGESNPVLKDWCMTVHEQATVGDRVNMAFMGTVVTTGRGKGIAVATGKETSMGM
                     IATSLSQVKREKTPLEIQLNHLGKVLSIVFLVVCAAVFVLGWLEMGNPGEQLLMAVSL
                     AVAAIPEGLPAVVTIVLSIGTTRMSKQGAIIRRLSSVETLGCTTYICTDKTGTLTENR
                     MKAVKVFYGMKHFDMSNAPKEEFGPLAKVALHCNDAAYNADGNLTGDPTETALIEFAE
                     EVGLDANSVSPRVDEIPFDSDRKAMTTVHKEKEQTVAYTKGAFEVVLHMSNKLYLNGK
                     VVDLDNETRKKLSEEGSKLTKEGYRTLGFAFKVAPVPEPGDKVEEHSLVLVGYIGLYD
                     PIRAEVPQAVQDCKDAGIVPVMVTGDHPETAIIYARKLGIATEDDKPITGKDFAENKP
                     GLDELIPKTKVFARVSPQDKLRIVEVLQKNSQVVAMTGDGVNDAPALKKADIGVSMGK
                     SGSEVAKDAASLILVDDSFGTIVKAVHEGRVIYDNILKFIWYMLGANIGEVLAIAFSL
                     IFMTGSPLAPIQLLWINLLTDGLPALALGVEPPESGIMKRMPRDPKQKFLSGKVLWGI
                     IFRAIAFGIAVYGSYLIGSARGGHILGSTYAFATTVITELLVGYTCRSFTKTIFEMKL
                     WGNPQLLGAIAVSLAMFSITFIPSLQPIFKTTGISMVDLGIIILMALIPPLFVDISKL
                     FLRRYAK"
     gene            5966..6556
                     /gene="gmk"
                     /locus_tag="PK717_02795"
     CDS             5966..6556
                     /gene="gmk"
                     /locus_tag="PK717_02795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR46623.1"
                     /translation="MQSSLFVLSGPSGVGKTTIRQELARMMPELFVSVSATTRQKRPD
                     EVDGSDYLFLGKEKFDVMLSENGFFEWANVHGNFYGTPTKPIEEKLACGKDALLVIDV
                     QGACQVRTRMPSSAILIFLLPPSINELESRLNGRKSDSIEQVKLRLENAKSELKSLLN
                     YDYSVVNHTIAQATHDVASIIRWCSYRRKYDRQGKN"
     gene            6534..6791
                     /locus_tag="PK717_02800"
     CDS             6534..6791
                     /locus_tag="PK717_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46624.1"
                     /translation="MIDRAKIEQFIKNMDGNKYLATIYLIRRVTGLQEKSSKLIPGSQ
                     RFMIEQSIDEFLEGKSKVSFAEDATCDIPKFVETQEGPKDK"
     gene            6791..>7027
                     /locus_tag="PK717_02805"
     CDS             6791..>7027
                     /locus_tag="PK717_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR46625.1"
                     /translation="MRSIALLIILSYLAGSFPSGYIVGWLFGKVDIRRVGSRQIGAAN
                     VTRQLGIKVGALSLSLDLLKGLLPMFITHYVLKQP"
BASE COUNT         2214 a         1510 c         1574 g         1729 t
ORIGIN      
        1 ccggaaacct cgactacgag aattcacttt ctgtgctcga tctccttctc aaaatcaaca
       61 caagagggac ctccatcctc atagccactc acgataaaaa catggtggag aacaccaaaa
      121 agaggatcat aaaactggat cacggacaga aagtgagcga cgcaaatgtt ctttcgtgag
      181 ctggcctggg catttaaatc aatcttcaga tccaaagcaa ggtcaacagc tgcaatagct
      241 gtcaccgcag tcactatttt tctcatgagc acaacccttc tccttggaat agccggggca
      301 aattttggca gaactgcgcg cgaaaggttt acgctccctg tttatttgag cccaagtgcg
      361 gattccaaca tcgcggaaaa catgtccgag caaataaagc tcttgccaaa tgtgaaggac
      421 gtaaaagtac tcactccgga tgacttgctg aaaaaagaag gtgaatcact ccatctttcg
      481 ccagaacaga taatccagtc catctcatac aatccgcttc catacagcct tgttgtggtt
      541 ccagaaaacc cacagaatat cgacgctctg gcaaaaagaa tcaaacagtt cacaatggtt
      601 gaggatgttg attacgaatc agatgtcata aacaagtttg tcaacctttt cgacttattc
      661 atctggatag ctcttggtct ggcagtgctt tccattctcg tctcaagtat tgtcattgga
      721 tcaaccatat cgctttcaat tgcatccagg attgaggaaa tcgaagtgat gagtttcgtc
      781 ggtgcttctc ctggcacaat cctcggccct ttcataatag aaggtgtttt ttatggattg
      841 attgggggtg gcatctcggc agctggaata tattttggct ggaaatatct ccagacaatt
      901 tttcaaacaa accttccctg gtttaatcta tcaatcgagc aaacagtttt ttattcaatt
      961 gtagctggaa ctgtcgtttt tggtttcctg accggtttca taacaagctg gctttcttcc
     1021 agaaacacac ttaggaggat aagagcttga aaaaaattgt acctgcactt ctagcgtgtc
     1081 tgattgcctt ttcgagcaca ccactgataa aggctggaaa tctccaggat gagctcagga
     1141 gtatcaacaa ccgcatcatc acaaacaaga gaaggctctc ggaaacaaaa aaatatcagg
     1201 atgtgctcgg tatcaaggaa aagtcaaagc tcgatgaaca agcaacagta aaagggctcc
     1261 ttgacgagta cgaaacaaaa accgccagta cccaacaaga gatagttgaa accgtaaaga
     1321 ggattgaaga agcaagaaaa aatatggaga caaagggaag agaactggac aaaaagcgtg
     1381 atgaggtcgg gaaactgctg acttttgttc acaagttttc ccaaaacagg tttatagact
     1441 atgttttttc tgcagagagt ttctccgagc taaccggaag ggcaagattc accaacaaga
     1501 tcttgaacgg gacagtaaaa gcaataactg atcttcacct ggaatcatca aggatagaaa
     1561 ctgcaagaca aaatctcgaa aaagacaagc agaggcttgc agagttaaaa accgagtaca
     1621 aaacccttgt cgaaagatat gggggcctca aaaaaaagaa cgatcaaatt ttgaatgata
     1681 tcaaaaacca gaaacagtgg acaaaggatg aactgataag gatacaacgg caacttgata
     1741 acgatcaggc ccaagtaaaa atcatcctca aaaaaattgc tgaagaggaa gaaagacgga
     1801 aaagggcggc ctacaaaggt gcttacattt ggccaatacc ggtaaacggt cccattggtc
     1861 ctttcggcat gagaaaacac ccaatctttg gtgatgtaag gatgcacacc gggattgata
     1921 ttgatgcaaa gatgggtgca ccaattatgg ctgctggaga tggctttgtt ggatttgttg
     1981 gtcagcttgg tggatatggc aatcttgtta ttctaagcca tggaaacggc ataacaacat
     2041 tttacgccca catgtcaagg tttgggtgct caaagggaca gcaggtcaaa aagggccagg
     2101 tcattgggta tatcggctca acaggctgga gcaccggccc acaccttcat tttgagtata
     2161 gaatcaacga taaacccatg aatcctttga gttatttgcc aaggtgagac gatgagagaa
     2221 aacagataca cattttgggt aacaatctcg acattcctga taatcgtttt tggtttggga
     2281 atattttgtg gagtgcttgt aggttccagg gcagcaaaat ccacgcaaac aaagatagac
     2341 atcaacaaca tgcctttgct gagcaaaact gttcaggtgg ttatggacaa ttactacagg
     2401 gctgacatgg tcacagaaga gaatcttgca aaagcaatgc tttttggcct tgatccatat
     2461 tccacgtttt ttaataagga cgaatacaaa ctttttcagg attccaccgt cggaaactat
     2521 gtcgggattg gtgtgacgat aggcaatgac cagtcaaaaa ggcatgtcat agttcttcag
     2581 gttttcgaca agtcccctgc ccttagggct ggtgtcgaag agggctggga aattgtaaaa
     2641 ataaacggct caaaactaaa aagtaccgac attgattatg tttcaagcct gttgaaaggc
     2701 aaagaaaaca caagcataaa cctcactttc caacatgatg gcctgtttgt tgacaaaaca
     2761 attgccagag agcagataac gatcaataca gtttcctcaa aaaatataga taaatacaca
     2821 tatataaggc tcagcaattt catgccacag agttttgagg aaatgaaaaa tgctataaac
     2881 aattttgagc tttcaggttc taaaggcttg atatttgatt tgcgcagaaa ccctggaggt
     2941 tttgtaaaat cgtgcattga catatcaaac ctattcctgg agccagggac actcgcattc
     3001 accaaagacc aggcgggaag aacggaggaa atcaaaacaa gcggcatcag atttgagaaa
     3061 ccacttgtcg ttcttgtcga tcacttcaca gcatcagcgt ctgaagttct tactggagca
     3121 cttcaagaca ggggtgttgc aacagtcata ggaacaagga cttttggaaa agggctcata
     3181 caaaacatca taccggtccc tccatttggc gtcatcaaac taacaataca aagttatctg
     3241 accccaaaca ggaacgaggt tgatgttgtc ggcatcaagc ccgatataga ggtttcagaa
     3301 ggtgttgaca acttctatcc acttgatcca aaggaagacc cttttatcca aaaagctatc
     3361 gaaatcctca aggagaaata aaacatgaac tggcatagta ttccactgga cgaaacccta
     3421 aaaacaatcg atgcaactct ccaaggtctc agctcgaaag attcaaaaaa caggcttgaa
     3481 aagtatggcc ccaatgaaat atctgcaaaa gagcgttcgt cagttctcca gatgatcgtt
     3541 gaacagttcg tgtcgccgct tatcctgatt cttttagctg catgcatatt ttcaatcatc
     3601 cagggcgaat gggtcgatgc catagcaatc ataaccgttc tggtggcaaa tgcgaccctt
     3661 ggaataatcc agcaatacaa atccgagggg gcactggaag cactcaaaaa aatgtctgcc
     3721 ccaaaggccc acgttttcag agatggctca atccatgaaa taccatcatc tgaggtagtt
     3781 cctggcgatc tagtaatact ttcagcagga gatcttgttc ccgccgatct aaggcttgtt
     3841 gaaacaatta accttgcatg caacgaagcg atcctgactg gggaatcaaa tccagtcctc
     3901 aaggactggt gcatgacggt tcacgaacaa gcaaccgtcg gcgacagagt gaatatggcc
     3961 ttcatgggaa cagtcgtaac aactggtcga ggcaagggaa ttgccgttgc tacaggcaaa
     4021 gaaacctcga tgggcatgat agccacaagc ctttcgcagg tcaaaaggga aaaaacgccc
     4081 cttgaaatcc agctcaacca ccttggcaag gtgttgtcaa tcgtattcct ggttgtatgc
     4141 gctgcagtat ttgtccttgg ctggctggaa atgggaaacc ctggagaaca gcttctcatg
     4201 gcagtttcac tcgccgttgc tgcaattcct gagggtcttc cagcagttgt aacaatcgtt
     4261 ctttcgatag gaactacaag gatgagcaaa cagggtgcca tcatcagaag gctctcttct
     4321 gtggaaacgc ttggttgcac aacctatatc tgcaccgaca aaacaggcac gcttacagaa
     4381 aacaggatga aggccgtcaa ggtcttctat ggcatgaagc attttgatat gtccaatgcg
     4441 ccaaaagaag agtttggtcc cctggcaaaa gttgccctcc attgcaatga tgccgcctac
     4501 aatgcagatg gaaacctcac tggagaccct acggaaacgg ctcttatcga atttgcggaa
     4561 gaagttggtc ttgacgcaaa ttctgtaagc ccaagagttg atgaaatacc atttgattca
     4621 gacagaaaag ccatgacaac tgtccataaa gaaaaggaac agactgttgc atacacaaag
     4681 ggcgcatttg aggtcgttct tcacatgtcc aataaactgt atctgaatgg aaaagttgtt
     4741 gatcttgata atgaaaccag gaaaaaactc tctgaagaag gctctaaact gacaaaagaa
     4801 ggctacagaa cgcttggttt tgcattcaag gttgcccctg taccagaacc tggcgataag
     4861 gttgaagaac acagcctggt tcttgttgga tatatcggtc tatacgatcc aataagggcc
     4921 gaggttccac aggctgttca ggattgtaaa gatgcaggaa ttgtaccagt catggtaact
     4981 ggcgatcatc ctgagacggc aataatctat gccagaaaac tcggcatcgc gaccgaggat
     5041 gacaaaccca tcacaggaaa agattttgca gaaaacaaac cgggactcga tgaactgatc
     5101 ccaaaaacaa aggtctttgc cagggtttcc ccgcaagaca agctcaggat agttgaggtt
     5161 ctacaaaaga attcgcaagt agttgcaatg accggtgatg gcgtcaatga tgcaccggct
     5221 ctcaaaaaag ccgatatagg tgtctcaatg ggcaagtcgg gatcagaagt agccaaagat
     5281 gccgcatctt tgatcctcgt tgatgatagc tttggaacca tagtcaaagc ggtacacgaa
     5341 ggaagggtca tctacgataa tatcctcaaa tttatatggt acatgcttgg cgcaaacatc
     5401 ggagaggtac tggcaatagc attttcattg atatttatga ctggttctcc tcttgctcca
     5461 atacaattac tctggatcaa cctgcttaca gatggtctcc cagcacttgc ccttggcgta
     5521 gagccgccag aatcaggaat aatgaagcgc atgccgagag acccgaaaca gaagtttctc
     5581 agcggcaaag tgctttgggg aattatattt agagctatag cctttggtat agctgtttac
     5641 ggctcatacc taattggttc tgcaagaggt gggcatatcc taggatccac ctacgctttt
     5701 gcaacaaccg tcatcacaga acttcttgtc gggtacacct gtagatcatt tacaaaaacg
     5761 atttttgaaa tgaagctctg gggcaatccg cagcttcttg gtgccatagc agtgtcattg
     5821 gcaatgttct ctataacatt cataccatca ctccagccaa tcttcaaaac aaccggcata
     5881 agcatggtag accttggaat aataatcctc atggcactca taccaccatt atttgtcgat
     5941 atcagcaagc tgtttctgag gcgctatgca aagtagcctt tttgtgttgt ccggcccgtc
     6001 tggagttggc aagaccacaa taaggcagga actcgcaaga atgatgccag aacttttcgt
     6061 ctcggtgtct gcaacaacca ggcaaaaaag accggatgaa gtggatggta gtgattattt
     6121 atttctgggc aaagaaaaat ttgatgtaat gctttcagaa aacggatttt tcgagtgggc
     6181 gaatgtccat gggaatttct atggcactcc aacaaaacca attgaggaaa aactcgcatg
     6241 tggcaaggat gcacttctag tgatcgatgt tcagggtgcc tgccaggtga ggacaagaat
     6301 gccatcctcg gcaatattga tttttcttct tccacctagc ataaatgaac tagaatcaag
     6361 gcttaatgga agaaaatcag atagcatcga acaggtaaaa ttaagacttg aaaacgcaaa
     6421 atcagagcta aaatctttgt tgaattatga ctactctgtg gtaaatcaca cgatagccca
     6481 ggcgacccat gatgtcgcaa gcatcataag atggtgcagt tacaggagga aatatgatag
     6541 acagggcaaa aattgagcaa tttatcaaaa atatggacgg taacaaatat cttgccacca
     6601 tttatttgat acgcagggtc accggcctgc aagaaaaatc ctcaaagctc ataccaggat
     6661 cacaaagatt catgatagag caatcaattg acgagtttct tgagggaaaa tccaaggttt
     6721 cctttgcaga agatgcaacc tgcgacatcc caaaatttgt agaaacccag gaaggcccaa
     6781 aagataagta atgaggagca ttgcgctgct catcatttta tcctacctgg caggctcttt
     6841 tccatcaggc tatatcgttg gctggctttt tgggaaagta gacataagac gtgttggctc
     6901 aaggcaaata ggtgctgcaa acgtaaccag acagctcggg ataaaggttg gagcgctttc
     6961 attatcactt gacctgttga aaggtctctt gccaatgttt attacccact atgtccttaa
     7021 acaaccg
//
LOCUS       DAOSRI010000062         6976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10030_length_6976_cov_4.291576, whole genome shotgun sequence.
ACCESSION   DAOSRI010000062 DAOSRI010000000
VERSION     DAOSRI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10030_length_6976_cov_4.291576"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK717_02810"
     CDS             complement(<1..484)
                     /locus_tag="PK717_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOR46626.1"
                     /translation="MSLGKIQVYTGCGKGKTTCAFGLAMRARGHKKSVLFVQFMKGSE
                     DYGELESSRVLGITVLQFGRPSFVDPKNPDPIDIGLAKEGLSFLKKATASGEWDIIVA
                     DEINVAIYFGLISLEETKKMLLDKYPGTEMVLTGRYAHPEIIEMADLVTEMKEIKHYF
                     K"
     gene            564..971
                     /locus_tag="PK717_02815"
     CDS             564..971
                     /locus_tag="PK717_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46627.1"
                     /translation="MPKSKAKKITIATGRIMVFLAVLAVLYFLLRPIAQIDYRVANLL
                     TEAKTLDESINSLKNDINTAKNELDINPGVYERLAREQWQMVHPGEMIYICKDDEGNN
                     ILSQSNLIFRERTEQKKPNPFVMLWNWILSLFR"
     gene            1022..1097
                     /locus_tag="PK717_02820"
     tRNA            1022..1097
                     /locus_tag="PK717_02820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1055..1057,aa:Met,seq:cat)
     gene            1205..1570
                     /locus_tag="PK717_02825"
     CDS             1205..1570
                     /locus_tag="PK717_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR46628.1"
                     /translation="MTTLGEFLNQQRLQKKVTFAELAKSCSMATPTIWKVIKDKARPK
                     DLTLQALSESLNIPLQELFRIRDKTRYPSTKRRTRRAAEEIYQNQSIGKEELMELIQN
                     SKISNQTIEAVYDLIQSIG"
     gene            complement(1571..4705)
                     /locus_tag="PK717_02830"
     CDS             complement(1571..4705)
                     /locus_tag="PK717_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR46629.1"
                     /translation="MIDFYKAISAIDPSLEIDKFVAKTLEVACSTMGFMSGVVIPIDS
                     EIGPVIHYNMPSDAMEIIRLWQNEYHKGDNFDFWDEPMIRRPMDKLAKLYGIKGAVIL
                     PLSVSNLILGDLVMFDTRRRTPTNDEKIMLEHVRTLLSITMANESRRYKTRFDPDIHE
                     RLFRGVANATNRLMTKGPLEEALSQAIGALGESTDIGMVNLSQFIKDEESGKLVVSVL
                     GEWRKNKPNTTQQNKRIAIIQPDFEQFFNTLSQGKTIKKLVRELPDREKLFFEEREIS
                     SFIVVPIFVDKKFWGGISFSDFNYERIWRKSDESILETMAATIGAFIDNKRNEEELGR
                     RESENRAILRAIPEMIFRVDFSGKLIDYKADNASDLIMPIDAKGKALEEILPENIAKI
                     TRENLEKAIVEGATKIYQYSVDTNNKTIYREARAVPYSNDQVLILAHDITEQREFMVQ
                     LEEAKKMAEEANRAKSEFLANMSHEIRTPLNALLGFVELLERNANSPESGNYLSAITT
                     AANAINTITADILDITKIEAGKLKLSLVPGSIKNIVEESVLLVKPKAELSKNSIEVEF
                     GAGSDKICLIDSPKLRQVMVNLLDNAAKFTKKGHIEIKVAAKKETKSKIDYEISVSDT
                     GIGIPWEMREKIFQAFMQVDGSYSRRFGGTGLGLAITRGILDAMGGTIKAIENEKNGT
                     TFVIGLSLSKAEGATIQNIQTPVRAFLHISSPQLKELVANLLAKVGIAVEESIWVEGS
                     GLIITENPKNDEQSEKWSKECNILTIGKDIPCFASNKSNKDDFIKSIYFSLAKLYPWN
                     YEIALKTIEDKKILLVEDNKMNIALLEEISKTIHCEMVSATSSLAAVNLALKNHFDVC
                     LMDIQMPDLSGLDATMQIRYNEDKEHGPRLPIIALTAFATEDDKKKCLEGGMDGYLSK
                     PIKIADLVTGMAQAISKTSPESKKDALSCLSEKLLIEKTRLKEMMAEYITGSLKNIKE
                     IKQHIQENDLKKASKCAHEIKGMAYDEDLFRIITDLESSLRLEDKAESSENTAILERE
                     LKNFSNRLGIKTEN"
     gene            complement(4702..6786)
                     /locus_tag="PK717_02835"
     CDS             complement(4702..6786)
                     /locus_tag="PK717_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46630.1"
                     /translation="MDYGLPARYKLRMLIDASKPFKIKIDAMYIGNGKWRPQDSNPEF
                     APETFYTEKVMFWTLPITSVFKNTLQPSKSSIISKIDVNVLRGHSEPFVLAITGGVNL
                     GDVEISLNKPLVSGNAQANVEFFTVSYMKKRFNINGPVSEKVEMAEYISKDSKLVIPE
                     GRTGFFWLKFSLPEQSQEGIFKGLLKIKNAATEDLYLPINLRAWNLDLPKNPECLMFQ
                     ATNHIKLVQKGKVSPDDGWKRYKSDFDEMASRGVNSVVLPVPLVSDPPYMPDLPKLEN
                     LAKMASSSGFNRFFMDITEAYGLAKKSAPEELKSELSRSLEQSLSTIRKIGLEPIIYI
                     GKTTSELDDGQKEMFSIATSINATPRTACHFTLWPDQSQVDFPIIDWAVIDYTVIQRQ
                     GKLPEGVIVNFQPSPHWGMAHQSRVFAGAYAYTHGLKYLSFGNYQQYYGDPFNDFDQM
                     LGDGSYTPGDSMWTYVNESYNVFHSIRWECLLEGLKDRQLLDLLNTKMMVSKGDSAVK
                     AGSLMTEALGTGNWQSLRLLSLVLDPAKMALFREKVIGFISWLSGGEKVAPFKTSYKI
                     DFVVGSKELKFGEEKLQMTVEPKIIGGSTYIPARYLVEPLGGKALWDAKTKTVSLVAV
                     SKHVNLVINSKKAIVDTEEVTLTNAPLIVDGRTLIPLRAASTLLGFGVEWNAQTRTAT
                     ILAEPKETYLGQ"
BASE COUNT         1692 a         1553 c         1492 g         2239 t
ORIGIN      
        1 ttttgaaata atgttttatt tctttcattt cagtaacaag gtctgccatt tcaatgattt
       61 ccggatgggc gtatcttcca gtcaagacca tctcggtccc agggtattta tcaagcaaca
      121 tttttttggt ttcttcaagg cttatcaggc caaaatatat ggctacgttt atttcatcgg
      181 caactatgat gtcccattct ccagaagcag ttgctttttt cagaaaggaa agcccttcct
      241 ttgcgagccc gatatctata ggatcaggat ttttgggatc aacaaatgat ggccttccaa
      301 attgcaggac cgtgatgccc agcactcgcg aagattcaag ctcaccgtaa tcctctgatc
      361 ctttcatgaa ctggacaaac aagacacttt ttttgtgccc tcttgccctc atcgccagcc
      421 cgaaggcaca tgttgtcttg cctttgccac agccggtgta aacttgaatt tttccaaggc
      481 tcattggtct caatcatagg cccacaaatg cacctgtcaa ctcaatactt gatttgatct
      541 ggctagggtc tagaatcttt cttgtgccaa aaagcaaggc aaaaaaaatt actattgcca
      601 ccggaaggat aatggttttt ctggctgtgc ttgcagtgtt gtattttttg ctcaggccca
      661 tagcccagat tgattacagg gttgcgaatt tgcttactga agcaaaaacc ctagacgaat
      721 ccattaactc gcttaaaaac gacataaata ctgctaaaaa tgagcttgac attaatccgg
      781 gtgtttacga acggctggcc agagaacagt ggcaaatggt ccatcctggt gagatgatat
      841 atatctgcaa ggatgatgaa ggtaacaaca tcttatcgca atctaatctt atattcaggg
      901 aaagaactga gcaaaaaaaa cccaatccat ttgtgatgct ttggaactgg attttatctt
      961 tatttagata aagcccttgt gtttcgggta gatttcatta acattttttg gagtgcatta
     1021 gggcgaggta gctcagcggt cagagcacgc ggctcatacc cgcggtgtcg tgggtccgat
     1081 tcccaccctc gccaccattt gtctgggtga ttctgtcgtt aggttgtcgt ttttttgttc
     1141 acatctagct attgcatttt tttaattgtt agtataggat ttgggcaaga tggcaaaaca
     1201 aacaatgacg actttaggag agtttcttaa tcagcagcgg ttgcaaaaga aagttacctt
     1261 cgcagaactt gccaagtctt gcagcatggc tacgccaaca atatggaaag taataaaaga
     1321 caaagcaagg cccaaagatt tgacactcca ggcgctttct gaatcgctga acattccttt
     1381 gcaggaatta ttcaggataa gggacaaaac ccgctacccg tccaccaaga gaagaacaag
     1441 aagggccgca gaagagatct atcaaaatca gtcaattgga aaagaagaac taatggaact
     1501 tatacaaaac tccaagattt ccaaccagac gattgaggca gtctacgacc tgatccaatc
     1561 gataggctga ttagttctcg gttttgattc ccaaacgatt gctgaaattt tttagctctc
     1621 tttcgagtat tgcagtattt tctgatgatt ctgctttgtc ttccaatctt aatgatgatt
     1681 caagatcagt gattattcta aaaagatctt catcataggc catccctttt atttcatggg
     1741 cgcattttga tgcttttttg agatcgtttt cctgtatgtg ttgttttatt tctttgatat
     1801 tttttaggct tcctgtaata tattccgcca tcatctcttt taatctggtt ttttctatca
     1861 acagcttctc agacaagcat gacagggcat ctttttttga ttcggggctg gtttttgaga
     1921 ttgcctgtgc cattcctgtg accaagtcgg ctatttttat cggttttgac agatagccat
     1981 ccatcccgcc ttcgaggcat ttctttttgt catcctcggt ggcaaaagct gttaacgcta
     2041 tgatcggcaa tctgggtcca tgttccttgt cttcgttgta tcttatctgc attgttgcgt
     2101 ccagcccgga caagtcgggc atctggatgt ccatcaggca aacatcaaaa tggtttttga
     2161 gtgccagatt gacggcagca aggctggagg tcgcagaaac catttcgcaa tggattgttt
     2221 tggaaatttc ctccaggagg gcgatgttca tcttgttgtc ttcgaccagc aggatctttt
     2281 tatcctcgat agtctttaat gctatttcgt agttccatgg gtagagcttt gcgagagaaa
     2341 aatagatcga ttttataaaa tcatccttgt tcgatttgtt gctggcaaaa cacggaatgt
     2401 cctttcctat cgttaatatg ttgcactctt ttgaccattt ctcagactgt tcgtcgtttt
     2461 tggggttttc tgtaatgatt aatcctgatc cttcaaccca aattgactcc tcaactgcta
     2521 tgccgacttt tgctaggaga tttgcaacaa gctctttcaa ttgcggggag ctgatatgca
     2581 agaatgccct gaccggtgtt tgaatatttt gtatagtggc accttcagcc tttgataatg
     2641 agagacctat tacgaacgtg gttccatttt tctcattttc tattgccttt atcgttccac
     2701 ccattgcatc caggattccc cttgtgattg caaggccgag tcctgttccg ccaaaacgcc
     2761 tggaatatga cccatcaact tgcatgaacg cctggaatat tttttccctc atctcccaag
     2821 ggattcctat accagtatca cttactgaaa tctcgtaatc aatttttgat ttggtctctt
     2881 tttttgctgc caccttgatt tcaatgtggc ccttttttgt gaattttgct gcattatcaa
     2941 gcagattcac catcacttgc ctgagttttg gtgaatcaat gaggcagatc ttgtcagagc
     3001 cagctccaaa ttctacttct attgaatttt ttgagagttc agcttttggt tttaccaaca
     3061 aaacagattc ttccacaata tttttgatgc tgcctgggac aagtgaaagt ttcagtttcc
     3121 cagcctctat ttttgtaatg tccaaaatat ctgcggtgat tgtgttgatg gcatttgctg
     3181 ccgttgtgat ggctgagaga taattgccag attcaggtga atttgcattt ctctcaagga
     3241 gctcgacaaa accaagcagt gcgttcagcg gagttctgat ctcatgcgac atgtttgcca
     3301 gaaattcact ttttgccctg tttgcttcct cggccatttt tttggcctct tcgagctgga
     3361 ccataaattc cctttgctct gtaatgtcgt gggccaaaat gagtacctgg tcattactat
     3421 aagggacagc cctggcttcc ctgtatattg ttttgttgtt tgtgtccacc gagtactggt
     3481 agattttcgt ggcaccttca actatcgctt tttcaagatt ttccctggtt atttttgcaa
     3541 tgttttctgg caggatttcc tcaagggcct ttccttttgc atcaatgggc attatcaggt
     3601 cgcttgcgtt gtctgctttg taatcaatga gctttcctga aaaatccacc ctaaagatca
     3661 tttctggaat tgcccttagg attgccctgt tctcggattc ccttcttccc agctcctctt
     3721 cattgcgttt gttgtcaatg aaagccccaa ttgttgcggc catggtttcc aatattgatt
     3781 cgtcgctttt tctccagatc ctctcatagt tgaaatcgct aaaactaatt ccaccccaaa
     3841 actttttatc aacaaatatt ggcactacaa taaaagaaga gatttccctt tcctcaaaaa
     3901 atagtttttc cctgtcaggc agttccctga ccaatttttt tattgtcttc ccttgggaca
     3961 gggtattaaa aaattgctca aaatcaggtt gaattatggc aatgcgctta ttttgttgtg
     4021 tagtatttgg tttgttcttt cgccattcac ccagcacaga aacaaccagt ttgccagact
     4081 cttcatcttt tatgaactgg ctgagattta ccatgcctat atctgttgat tcgccgagtg
     4141 cgcctattgc ctgggaaagt gcctcttcga gcgggccttt tgtcattagc ctgttggtgg
     4201 cgtttgcaac acccctgaag agcctttcat gtatgtcagg atcaaacctg gttttgtatc
     4261 tcctggattc attggccatt gttatggaaa gcagcgtcct aacgtgttcc agcattatct
     4321 tttcatcatt ggtgggggta cgtcttcttg tgtcgaacat tacaaggtcc cccagaatca
     4381 gattggagac actcaaagga agtattacgg cacccttgat tccgtagagc tttgccagtt
     4441 tgtccattgg ccttcggatc atcggttcgt cccagaaatc gaaattatcg cccttgtggt
     4501 attcattttg ccacaggcga attatttcca tggcatcgct tggcatgttg tagtggatta
     4561 ctggcccgat ttctgaatct atcggaataa caacacctga catgaaaccc attgtggagc
     4621 aggccacttc aagcgtcttg gcgacaaact tgtcaatttc gagtgatgga tcaattgcag
     4681 atattgcttt gtagaaatct atcattgtcc cagataagtt tcttttggtt ctgcaagaat
     4741 tgtggccgtt ctggtttgcg cgttccactc gaccccaaat ccaagcagag tcgatgctgc
     4801 cctgagtggg atgagggttc ttccgtcaac aatgagcgga gcattcgtca aagtgacctc
     4861 ctccgtatcg acaatggctt tttttgagtt tatcacaaga ttcacatgtt ttgaaactgc
     4921 aaccagcgaa actgtttttg ttttggcgtc ccagagcgct tttccaccca agggctcaac
     4981 cagatacctt gccggtatat atgtggaacc accgattatc ttgggttcaa cagtcatctg
     5041 gagcttttct tccccgaact tcaattcttt ggagccgacc acaaaatcta ttttatatga
     5101 tgtcttgaaa ggcgcaactt tttcaccgcc agagagccat gaaatgaacc cgattacctt
     5161 ttctctgaac aatgccattt tggcaggatc aaggacaagc gaaagtagtc tcaatgattg
     5221 ccagtttccg gttccaaggg cttctgtcat tagagagcct gctttgactg ctgaatcacc
     5281 tttggacacc atcatttttg tattcaacag atcaagcaat tgcctgtctt taaggccctc
     5341 cagcaaacac tcccacctga tggaatggaa gacattgtat gattcgttta catatgtcca
     5401 cattgagtcg cctggcgtgt aggagccatc gccaagcatc tggtcaaaat cattaaaggg
     5461 gtctccgtaa tattgctggt agttgccaaa agaaagatat ttcaatccat gtgtataagc
     5521 gtaggcaccg gcaaaaactc tcgactggtg ggccattccc cagtggggcg atggctggaa
     5581 atttacaatc acaccttcgg ggagtttccc ttgccgttgg atgactgtat agtcaatgac
     5641 tgcccagtca attattggga aatcaacctg ggactggtcc ggccacaatg tgaagtggca
     5701 ggccgttctt ggcgtggcgt tgatggatgt ggctatggaa aacatttctt tttggccatc
     5761 atcgagttcg cttgtggttt ttccgatgta gattataggc tcaaggccaa tcttccttat
     5821 ggttgacagc gactgctcaa gtgaccttga aagctcggat ttcagttctt ctggggctga
     5881 tttttttgcc agcccgtagg cctcggtgat gtccatgaaa aacctgttga agcctgatga
     5941 agaggccatt tttgcaaggt tttccagttt tggcaggtcc ggcatgtatg gtgggtcaga
     6001 aacaagcggt accggcaaaa caactgaatt cacgcctctt gatgccattt catcaaagtc
     6061 cgatttgtag cgcttccagc catcatcagg ggaaactttt cctttttgga caagctttat
     6121 gtggtttgtg gcctggaaca tcaggcattc cgggtttttg gggaggtcga ggttccatgc
     6181 cctaagattg atgggcagat agaggtcttc ggttgcggca ttcttgattt tcaggagtcc
     6241 cttgaatatt ccttcctggg attgctcggg taggctgaat tttagccaga aaaagcctgt
     6301 cctgccttct ggtatcacaa gttttgagtc ttttgagata tactcggcca tttcaacttt
     6361 ttcgctgacc ggcccattga tattgaaccg ttttttcatg tatgagacgg tgaaaaattc
     6421 aacatttgcc tgcgcatttc ccgaaaccag cggtttattg agggaaatct ccacatcacc
     6481 caggtttact ccaccagtga tggcgaggac gaatggttcg gagtggcccc ttaacacatt
     6541 cacatcgatt ttggagatga tggatgattt tgacggttgc agggtattct tgaaaactga
     6601 agtaattggc agtgtccaga acattacctt ttcggtgtaa aaagtttcag gtgcaaactc
     6661 tgggtttgaa tcctgcggcc tccatttgcc gttgccaatg tacatggcat cgatttttat
     6721 tttgaacggt tttgatgcgt ctataagcat cctcagtttg tacctggccg gtagcccgta
     6781 atccatccca tccgagaccc cgaagcgctc ccactgaaaa ccattaccgt atgtattgcc
     6841 tttttcaatg aaaactgatt gcctgttttg tgtttttggt gactgggtat caagattttc
     6901 tgcctcgaag acaacttctg gcttttcacc aacaaatgaa catgaaagcc agaactactc
     6961 gaatgagcag atattt
//
LOCUS       DAOSRI010000063         6946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10082_length_6946_cov_3.492091, whole genome shotgun sequence.
ACCESSION   DAOSRI010000063 DAOSRI010000000
VERSION     DAOSRI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10082_length_6946_cov_3.492091"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK717_02840"
     CDS             <1..673
                     /locus_tag="PK717_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46631.1"
                     /translation="NIAKFINSPYSYLVPTLSGVAIIAIIVVIGLYFSRSIAEMKTIT
                     PRELYGFAVGYIMVEPLLSAYTGFTSLFGIGSSNVPVMYGAPLPGVYPSTSLAIYDLT
                     VPFLQRGMGMVITLALVIWVLWSFRRQTHEGMKTWAVFTLAIVAKIVLDVLMSYFNVL
                     PVLGVSQASMLEQFPFVSSVTQGIVITLIIFVGFFAVLLYRQKLARLSQEACAVTIDQ
                     LRAQK"
     gene            683..1033
                     /locus_tag="PK717_02845"
     CDS             683..1033
                     /locus_tag="PK717_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46632.1"
                     /translation="MYINWWFYSFWFIVCFAFAMFLLPLIVKAFSGEKVPTQANLYAV
                     ITTTLFTLFMFSLFGQGYLWMVIFNVLLLVFFQFWWPIQGQSKFKAWGIYAATNCVFQ
                     IFIFWLFILVKLGA"
     gene            1038..2669
                     /locus_tag="PK717_02850"
     CDS             1038..2669
                     /locus_tag="PK717_02850"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOR46633.1"
                     /translation="MKLIKEIAGVLGLTEDDIELYGKYKAKISYATAKKYGSEKKGKL
                     IIVTAINPTPFGEGKTTTSIGLGDALRKIGLNSIICLREPSLGPCMGVKGGAVGGGKS
                     LIVPSSDINLHFTGDIHAVTTANNLLSSIIDNFIKYDLEPNIDIRHVPWKRCVDLNDR
                     CLREVIVGLGGQTNGVVRDEGFVISVASEIMAILCLSDDLADLKVRLGRIVCAYGNDD
                     KPITPKDLKCVGALAALMAEAIKPNLVQTIEGTPVFIHGGPFANIAHGTNSVIATRLA
                     LGLGDYIVQETGFGADLGCEKFLDIVAPSKGLPPDAIVIVASTRALKYNGGVGKKTVL
                     EPNEQAIRLGFANLARHIENIKKFGITPQIAINRFPTDLDSELQLICSLCEQMGASAA
                     IISNYEQGGQGAIGLAKNVVEGIKNNPPNYKPIYDYSLPIKEKITAVAKEIYRATKVN
                     FTSRASSDIKRIEEMGYGNLPVCMAKTQYSFSDNEDLLGAPTGFEVTVREAKVSAGAG
                     FVVAICGDIMLMPGLPKEPAAEHIDVDDNGVITGI"
     gene            2679..3530
                     /locus_tag="PK717_02855"
     CDS             2679..3530
                     /locus_tag="PK717_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOR46634.1"
                     /translation="MEILLDGKLLAEKMDAEFALRVEKIRKNGLPVKLTAILVGNDPA
                     SETYVRMKGKRCNKLGMGSETINLPEGTTQQQLLELIDKLNKDKETTGILTQLPLPKH
                     INEQVVLEAIDPSKDVDCFHPYNVGRLSIGSPVFLPATPAGIIALLERYGATLSGKRA
                     VVIGRSNIVGKPVSMLLLSKNCTVTVCHSKTADLPSVVREGDIVVAAVGKAEMVRGDW
                     IKPGAWVVDVGTNKLEDGRQVGDVAFDEAKEVAYAISPVPKGVGPMTITMLMENTIRS
                     AELRANF"
     gene            3530..4372
                     /locus_tag="PK717_02860"
     CDS             3530..4372
                     /locus_tag="PK717_02860"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOR46635.1"
                     /translation="MKIGIFIRKITESIVMTAIDSVRWLQDRGISVSAPEDVAVHLGM
                     NADPDFPKGCDLVLSLGGDGTLLRAIHKTAPYAIPVLGINLGHLGYLTEVEGSWLRNS
                     LIRIISNQYDLERRFMIRGTLMREGKELLTIDAVNDIYLYRNILSQVVDVEAYVGEEK
                     VSTTRADGLIVFTPTGSTAYALSAGGPIIDPECEVIGMISICPHRIDQRPIIIPNSKP
                     LRLVFDMSYGPASIFADGDIVSQLRPQDELTVTRSPYNAVFAKFGQKEFYRVLQIKFN
                     WGKR"
     gene            4376..6100
                     /gene="recN"
                     /locus_tag="PK717_02865"
     CDS             4376..6100
                     /gene="recN"
                     /locus_tag="PK717_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOR46636.1"
                     /translation="MLTGLSVSNFAVAKNITLDLDPGLTVLTGETGAGKSILVGALLV
                     GLGGRWQQEYLRDGADKSIVELVFHLGDSQRDLLPDSSVLEDGEIVLSRTIDSEGKSR
                     VRIGGSLANLTALKETSSRLVDVHSQFEAQSLLDPKVHLLMLDRYAGPDNLAILDEFR
                     AQAKKFDALLEELVQIRTSENDRRQRIDYISYELDQFEQVSPQDSEDESLNAERTRLS
                     NIQRLADLAQAIRLSLEGSDEQQGIRSLNRIAQRSFADLSRIDPKASEIGGMIDIIEA
                     NAQEALFFISSYINSLTFDPDRLQVVEDRLAVLGKLTRRFGSSLADAFEGVEKLKKEL
                     ERLSKSEERLSTIENEIELERKNLSLLGEKLVRSREKAAQRLSEEVVAQLGDLALEKA
                     KFSVQFQLLPPGEDKYIDIFGEKCGFGGTGPQYCEFVISTNPGEKEKSIQKVASGGEL
                     SRIMLALKVILSEVDQTPTLIFDEVDTGVGGRVGEMIGRKLAKLSQRRQVICITHLPQ
                     IACFGDIHLLIQKTQGEDETTIACLELARRGRVDELARMLSGDRVTPISLKHAEEMLL
                     QAQKIKGQ"
     gene            6097..>6946
                     /locus_tag="PK717_02870"
     CDS             6097..>6946
                     /locus_tag="PK717_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR46637.1"
                     /translation="MKDARQRAAEEASELRKKLDRANHQYYVLDSPEISDAEYDSMFR
                     RLVEIETEYPELITPDSPTQRVGGKPSEKFETVTFSVPMLSLGNAFDLEDLTAFDQRV
                     HKVAPDCTYTTEMKLDGLAINLRYERGVLVWGATRGDGNTGENVTSNLKTIKSIPLRL
                     NGDVPDILEVRGEVLMKEEELERLNQERESMELAPFANARNASAGSIRQLDPKITASR
                     KLEFFAYHIGECSEKFAETQTGILERFNGYGFVVVPEHKRAKSIRECWNNVQGIQEHR
                     EKYPYGA"
BASE COUNT         1897 a         1583 c         1789 g         1677 t
ORIGIN      
        1 caacatcgca aagtttataa acagcccata tagttatctt gttccaacgc tctccggcgt
       61 tgccataata gcaatcattg ttgtaatagg actctatttc tccaggtcaa tagccgagat
      121 gaagacgata accccaagag agctgtatgg ttttgcagtg ggttacataa tggtcgagcc
      181 attgctgtca gcgtacactg gcttcacaag cctttttgga attggctcca gcaatgttcc
      241 tgtcatgtat ggagcaccac tgccaggtgt ctatccttca acctcgcttg ccatctatga
      301 tctgactgtt ccttttctcc agagagggat gggaatggtg ataacacttg ctcttgtgat
      361 ctgggtcctc tggtctttca gaaggcagac gcacgaagga atgaaaacct gggccgtttt
      421 tactctggcg atagttgcaa agattgtcct ggatgtactg atgtcctact tcaatgttct
      481 tccagtgctt ggggtcagcc aggccagcat gctggaacag tttccgtttg tttcatctgt
      541 tacccagggt attgtcataa ctttaatcat ttttgtaggt ttttttgccg tgctgctata
      601 ccgccaaaag ctggccaggc tctcccagga ggcctgcgct gtgaccattg accagctccg
      661 cgcgcagaaa taggaggact aaatgtacat taactggtgg ttttactctt tctggtttat
      721 cgtgtgtttt gcgtttgcca tgttcttgct ccctttgata gtaaaagcgt tttcaggcga
      781 gaaagtacca acccaggcaa acctttatgc tgttattaca acgacattgt ttacgttgtt
      841 catgttttcg ttgtttggac agggctatct ctggatggtt attttcaatg ttcttctcct
      901 tgtctttttc cagttctggt ggccaatcca gggacagagc aagtttaagg cttggggcat
      961 ttatgccgct acaaattgtg tttttcaaat ttttatattt tggcttttca tactagtaaa
     1021 acttggagct tgaaaaaatg aaactgatta aggaaattgc tggcgtcctc ggactcactg
     1081 aagatgacat agaactttac ggcaaataca aagctaaaat ctcctatgcc actgcaaaaa
     1141 agtacggatc agaaaagaaa ggcaaactga tcattgtcac tgcaatcaac cccactccat
     1201 tcggggaagg aaagacaacc acctcgatag gtcttggtga tgcattgagg aagattggtc
     1261 tgaattcaat aatttgcctc agggagcctt cgcttggccc ctgcatgggt gtgaagggtg
     1321 gcgctgttgg tggaggaaag tcgcttatag tgccaagttc cgacatcaac ctccacttca
     1381 ctggtgacat tcatgcggtt acaacggcaa ataaccttct ctcctcgata attgacaact
     1441 tcattaagta cgaccttgag ccaaatattg atataaggca tgtcccgtgg aaaaggtgcg
     1501 ttgacctcaa tgacagatgt cttagggaag tcattgtcgg tcttggaggg cagacaaatg
     1561 gtgttgtaag ggatgaagga tttgttattt cagtagcttc tgaaatcatg gcgatccttt
     1621 gcctttctga tgatcttgcc gatcttaaag tcagacttgg aaggattgtc tgcgcctatg
     1681 gaaatgacga caagccgata acgccaaagg acctgaagtg tgttggcgcg cttgccgcac
     1741 tgatggcaga agcgatcaag ccaaaccttg tacagacaat tgaaggcacc cctgtgttca
     1801 tacatggtgg cccatttgcg aatattgccc acggcacgaa ctctgtcata gcgacaagac
     1861 tggcccttgg ccttggcgac tacattgtcc aggaaaccgg cttcggtgct gatcttggat
     1921 gcgagaaatt ccttgatatt gttgctccaa gcaagggtct accaccggat gccattgtaa
     1981 tagtggcctc gacaagggca ctcaagtaca atggcggagt tggcaaaaag actgtcctgg
     2041 agccaaacga gcaggccata agacttggat ttgcaaacct tgccaggcac attgagaaca
     2101 tcaaaaagtt tggaatcacg ccgcagattg ccattaacag gttccctaca gaccttgatt
     2161 ccgagctcca gttgatctgc tcgctgtgcg agcagatggg tgcaagcgcc gcaataatca
     2221 gtaactatga gcagggcggc cagggtgcca ttggtcttgc aaagaacgtt gttgagggaa
     2281 taaaaaacaa tccgccaaac tacaagccaa tctacgacta ctcgctcccg ataaaagaga
     2341 agattacggc agttgcaaag gaaatctatc gcgcaacaaa ggtgaatttt acttccagag
     2401 cctcttccga catcaagcgc attgaagaga tgggctacgg caatctacca gtttgcatgg
     2461 caaaaaccca gtacagcttc tctgacaacg aggaccttct tggtgcgcca acaggttttg
     2521 aagtcaccgt gagggaagca aaggtctctg ctggtgccgg atttgtggtt gcgatatgtg
     2581 gcgatataat gcttatgcct ggtctcccca aagagccggc cgccgagcac attgacgtag
     2641 acgacaacgg cgttataact ggaatctgag gaggcacaat ggaaatcctt cttgacggca
     2701 aactgcttgc cgaaaaaatg gacgcagaat ttgctctaag agtggagaaa atccgcaaaa
     2761 acggtcttcc ggtcaagctg accgccattc ttgttggcaa cgatcccgcc tccgaaacgt
     2821 atgtcaggat gaagggtaaa aggtgcaaca aactcggcat gggttcagaa acaatcaatc
     2881 ttccggaggg aacaacccag cagcaactgc tcgagctgat tgacaaattg aataaagaca
     2941 aggaaacaac aggcatcctg actcagctgc cattgccaaa acacatcaat gagcaggtgg
     3001 tccttgaggc aattgaccct tcaaaagacg ttgattgctt ccacccgtac aatgttggca
     3061 ggctctccat cgggtcaccg gttttccttc cggcaacacc ggctggaata atcgcccttc
     3121 ttgagagata cggtgcaacc ctttccggaa aaagggccgt tgtgattggc aggtcgaata
     3181 ttgttggaaa gcctgtttca atgctgcttc tttcaaagaa ttgcacggtc accgtctgcc
     3241 actcgaaaac agccgatctc cccagcgttg tgagggaagg ggacattgtg gtcgcagcag
     3301 ttggcaaggc agagatggtc aggggcgact ggataaagcc gggtgcctgg gtcgttgatg
     3361 taggcaccaa caagctcgaa gatggcagac aggtcggaga cgtggcattt gatgaagcaa
     3421 aagaagttgc atatgcaatt tctccagttc caaagggcgt tgggccaatg acaatcacga
     3481 tgctcatgga aaatacaatc agatcagcgg agttgagagc aaacttttaa tgaagatagg
     3541 catcttcatc agaaaaatca cagagtcaat cgtcatgact gcgattgact ctgtaagatg
     3601 gctgcaagac agggggatca gtgtcagcgc cccggaggat gttgcagtcc atcttggcat
     3661 gaacgcagac cctgattttc ctaaggggtg tgatttggtg ctttcgcttg gtggtgatgg
     3721 caccctgctg agggcaatcc acaaaacggc cccgtatgca atccccgtgc ttggtataaa
     3781 tcttggccat ctgggttacc tgaccgaagt tgaaggctca tggctcagga attccctcat
     3841 cagaattatc tccaaccagt acgatctgga gcgcaggttc atgataaggg gtaccctcat
     3901 gagagagggc aaggagctcc tcacgattga tgctgtaaac gatatatatc tttacagaaa
     3961 cattctctcc caggtggtcg atgttgaagc gtacgtgggc gaggaaaagg tttcaacaac
     4021 aagggctgat ggtcttattg tctttacccc cacgggatcg acagcttatg cactttctgc
     4081 tggcggacca ataattgacc ccgaatgcga agtcataggg atgatctcca tatgcccaca
     4141 cagaatagac caacgcccga taataatccc gaacagcaaa cccttgaggc ttgtttttga
     4201 catgtcttat gggccagcat ccatttttgc cgacggagac attgtttccc agcttcgtcc
     4261 gcaagacgag ctgactgtga caaggtcacc gtataacgcc gtttttgcaa aattcggcca
     4321 aaaagagttc tatagggtcc ttcaaataaa attcaactgg ggaaaaagat aggcgatgct
     4381 tacagggctt tcggtttcaa attttgccgt ggccaaaaat attacccttg atctcgaccc
     4441 aggcctcaca gttctgacag gagaaacagg agctggaaaa tctattctgg tcggcgccct
     4501 cctcgttggc ctcggtggca ggtggcagca ggagtacctg agggatggcg cagacaagtc
     4561 gatcgtggag cttgtttttc atctgggtga ttctcaaaga gaccttttgc cagactcaag
     4621 cgtccttgag gatggcgaaa ttgttctttc gaggacgata gactctgagg gcaagagcag
     4681 ggtgaggatc ggcggttccc ttgccaacct tactgcgctc aaggagacat catcaaggct
     4741 cgtggatgtc cactcgcagt ttgaagcaca atcactcctt gatcctaaag tccatctttt
     4801 gatgctggat agatatgccg gcccagacaa tcttgccatt cttgacgagt tcagggccca
     4861 ggcaaaaaaa tttgatgccc ttctggagga acttgtccag ataagaacaa gcgagaacga
     4921 cagacgccag aggattgact acatctctta cgagcttgac cagttcgaac aggtttcacc
     4981 gcaagatagt gaggatgaaa gtctgaatgc cgaaagaaca aggctgtcaa acatccagag
     5041 acttgccgat cttgcgcaag caataaggct gtcgctcgaa ggctccgatg aacagcaggg
     5101 aataagatcc ctgaaccgca tcgcgcagag atcttttgct gatctttcca gaattgaccc
     5161 aaaggcctca gaaattggtg ggatgattga tattatcgaa gcaaacgccc aggaggcatt
     5221 gttttttatt tcgtcataca ttaattcact gacttttgac ccggacaggc tacaggttgt
     5281 tgaggacagg ctggcagttc tgggaaaact gacaaggcgc tttggcagtt cgcttgccga
     5341 tgcctttgag ggcgtcgaga agctcaaaaa agaactggaa agactctcaa aatcagaaga
     5401 gaggctttca accatagaaa atgaaataga gcttgaaaga aaaaaccttt cacttctcgg
     5461 agaaaaactt gtcaggtcaa gagaaaaggc cgcacagagg ctctctgaag aggtggttgc
     5521 gcagcttggt gacctggctc ttgaaaaggc aaagttcagc gtccaattcc agctgcttcc
     5581 gccaggtgaa gacaagtaca tcgacatttt tggagaaaaa tgcggttttg ggggcacagg
     5641 ccctcaatac tgcgagtttg tcatttcgac aaaccctggt gaaaaggaaa aatccataca
     5701 gaaagttgca tcaggcggag agctttcaag gataatgctt gcactgaagg tcatcctgag
     5761 tgaagttgac cagaccccga cgctgatttt cgatgaagtc gacactggcg ttggtggcag
     5821 ggtcggggag atgattggaa gaaagcttgc caagctgtca cagaggaggc aggtaatctg
     5881 tatcacccac ctcccacaga tagcctgctt tggggacatt catcttctta tccagaagac
     5941 acagggcgag gatgaaacca ccatcgcgtg ccttgagctt gcaagaaggg gcagggtgga
     6001 cgagcttgca aggatgctca gcggcgacag ggtgacgcca atttcgctga aacatgcgga
     6061 ggagatgctc ctccaggcgc aaaaaataaa gggccaatga aagacgccag acaaagggct
     6121 gccgaggaag cttcagaatt aagaaaaaag ctggacaggg caaaccacca gtattatgtg
     6181 ctggattcgc ctgaaatatc cgatgcagaa tatgacagca tgttcaggag gctcgtagag
     6241 atcgagaccg agtatcctga acttatcaca cccgattcac caacacagag ggttggtgga
     6301 aagccatcag aaaaattcga gacagtaact tttagtgtcc ccatgctctc gttgggtaat
     6361 gcttttgacc tggaagatct gacagccttt gaccagaggg tccacaaggt tgccccggat
     6421 tgcacctaca cgaccgaaat gaagctcgat ggactggcga taaacctcag gtatgaaagg
     6481 ggtgtcctcg tctggggtgc aacaaggggt gacggaaaca ccggtgaaaa cgtcacctca
     6541 aacctcaaaa ccataaaatc gatcccgttg aggctgaacg gcgatgtccc ggacatactc
     6601 gaagtcaggg gcgaggtgtt gatgaaagag gaggagctgg agaggctcaa ccaggagagg
     6661 gagtccatgg aattggcacc ctttgccaat gcaaggaacg cctctgccgg ttcgatacgc
     6721 cagcttgacc cgaagataac tgcctcaagg aagttggaat tttttgcata ccacatcggg
     6781 gaatgctctg aaaaatttgc cgagacgcaa actggcatac ttgaaagatt caatggctat
     6841 ggttttgtgg ttgttcccga gcacaagagg gcaaaaagca tcagagaatg ctggaacaat
     6901 gttcagggaa tacaggaaca cagggaaaaa tatccttatg gtgcag
//
LOCUS       DAOSRI010000064         6942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10090_length_6942_cov_3.620880, whole genome shotgun sequence.
ACCESSION   DAOSRI010000064 DAOSRI010000000
VERSION     DAOSRI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10090_length_6942_cov_3.620880"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="ccsA"
                     /locus_tag="PK717_02875"
     CDS             complement(<1..607)
                     /gene="ccsA"
                     /locus_tag="PK717_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOR46638.1"
                     /translation="MLFGRILSVLTPLAMVPSIIYCLMFTPKEATMGDLYRIIYFHIP
                     CAWVATLAFFISFIFSIAYLAKRKAVYDSVAHRSSEIGLVFCVLATITGSVFATYTWG
                     SPWNWDPRETSIVFLLMIYIAYLLLRWLIEDRDKKAAISAGYSIVAFLATPFLVFVMP
                     RITSSLHPEQVGGLGLTKPMLYGVFGMLAGFTFLYLWILSLS"
     gene            complement(611..1264)
                     /locus_tag="PK717_02880"
     CDS             complement(611..1264)
                     /locus_tag="PK717_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOR46639.1"
                     /translation="MRSFRALLAKEIKVAFRGRHLVGVGFGLAIVLSATLGFSLAGTK
                     VSERVIASLLWIVVMFSSSDTLSRSFAKEEDEGTADQLRLRVPMPSVFLAKTLANAFF
                     VLLIACVVFPLLCFWLGVRFSALPLVGLLVLIGCPALSFAQGFVGYLMSRGRALSAAY
                     PVIVMPVILPLLVCLVLATSQALAEGTFSINLVVVIISESLAALVAGLVLVPYIEEE"
     gene            complement(1251..1820)
                     /locus_tag="PK717_02885"
     CDS             complement(1251..1820)
                     /locus_tag="PK717_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR46640.1"
                     /translation="MVASNLSKSFGSKMVFAGVSFDIEDEVWAIIGPNGSGKTTLLRI
                     MAGLLKTSSGTMEWFDRKKKYEKFQLSGRIAMCSPDLGLYKEMTVFENARFFANLAGV
                     PVPDFGVWGLSCHLEKRYQELSAGLKQRAKLMTAFAMARDVILLDEPEQHLDAEGKAI
                     LDNMVSSFGRLVVVATNNPWRDWRKIAQF"
     gene            1918..3093
                     /locus_tag="PK717_02890"
     CDS             1918..3093
                     /locus_tag="PK717_02890"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOR46641.1"
                     /translation="MSKGYILSAARTPIGKFLGSLSPFSAPKLGGFAIKAAVERAGVE
                     KERIDEVIMGQVVQAGCGQNPARQALIHAGLNPETNALTINKVCGSGLKAIMLASQSI
                     QLGEADIVVAGGMESMTNAPYYLEKARTEGYRYGDGTLVDGVIKDGLWCAFNGSHMGN
                     LAEYTAKKGNVTREEADLFAFNSQDRAIKAIETGIFDEEVAPIAIPQKKGDPIIFSKD
                     ETPRPTTMDTLQKLRPAFDKEGVVTAGNAPGLSDGGSAVVVASENVAKNHKPLASIID
                     YATAHTEPKELFWAPVFAVKKLMEKTGLKISDFDLIEANEAFAVQCIVDGRGLEWDPN
                     TVNISGGAIALGHPIGSSGSRIIATLIYNLKRNGKKRGLATLCLGGGGAVAMAIELV"
     gene            3104..3964
                     /locus_tag="PK717_02895"
     CDS             3104..3964
                     /locus_tag="PK717_02895"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOR46642.1"
                     /translation="MEIKKVGVIGAGVMGNGIAHVFAQTGYDVVLAEANIQLAEKGKA
                     TIQKNLGRMVQKGTITQEVADATLARIKTTENLEDLRDCQIVVEAVTENRQVKNQIYD
                     KLNGILQGNAILASNTSTISITELAAKYKNPGKFIGMHFMNPVPLMKLVEVIRGMLTT
                     DETYNIVNDLSIKLGKTPAVANDYPGFVSNRILMPMINEAVYCLMEGVADKESIDTVM
                     KLGMNHPMGPLALADLIGLDTCLAIMEVLHEGLGDSKYRPCPLLRKMVAAGLLGRKSG
                     KGFYDYTEQK"
     gene            complement(4078..>6942)
                     /locus_tag="PK717_02900"
     CDS             complement(4078..>6942)
                     /locus_tag="PK717_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HOR46643.1"
                     /translation="INFDASSANIPPVTANITAYFASREQIGPSIHSGVMIGGGTNGG
                     DRIAFSYDNSPFTPLAGFGGDASFNNMPTATQGVFTDGVVLGYQYFGAGAPYPEYDYV
                     NTGRFYYVHQFTTSFNRLNQDIPPFVGKYGFAMIAQSTDFGHPRDGEGQIRKNYFCQF
                     VHDSSPGAAGITRLKYRPDYIPDNSRLVACYLYANSVGRVIEDPDGSWLGQPIFDQRR
                     DSGDEVREHIPILVNPGREGNPQSLLCTGSFLGRNNDSGYRPSCGCLGWGQMQSTFRF
                     NITPYIADPYDIIHVTNLVQEYEPGNGQLYGFEIVMIFENSTLPLSQIMIADGALTLC
                     NNDRQNSQTMFSGFRTPPDPSTWPVDGANAGAYVGIIGNSADNGTCGSQLGWYRAEWD
                     YWACGTNRIPTDRFWLNPQPADPTAGQGGAWWANGWIGSNANPETPPTLPPGFPAGYL
                     WSPKAMNPRYASNAKQYSCSWFWHRNFTVVAAKTAIHWKADQWWDYVNQDYDTKALHR
                     PTRTYLAGEDSSVLLRPYLVCANTGGSDPNSLDDGQGPCVATEWGNDYTDCDPTTENN
                     YNFCAGSPAGGPWPNPYYPRSDERGHMHGFILQVPVVPSLQITKKCVPDPVLPDSLLI
                     YTITVTNDTPYTQTNVNIVENYPKGVTFYDANPPPDVDNNRWTTSVGLAGNGSLAPHE
                     TFVLVVRVRVGHLAKGTKLTNNVLTYADTAPNPEFAQCTVDVLGEPLITLTKTTKKFM
                     AQQGEKVSYTIIVKNIGTREATNVVVVDILPRELEYVASIPSGSLGMQKIVWSFGIMN
                     PGEVKIIELTLRVRKDISLNPGINIINTAIVTSAEGLRDQDTAVIVLRVGAKEPVVCE
                     CPDISILIPEAKPDNDNIYVIPASGTMTIKVSPFGGCGPYTADLDFEGDGKIDKTFNI
                     ENDTTKEFSYTYSPGSYSITLRTTDRYANSCTYTKKIRVK"
BASE COUNT         1972 a         1661 c         1653 g         1656 t
ORIGIN      
        1 gagacaaaga cagtatccat agatacaaga atgtaaaacc agcaagcatg ccaaacacac
       61 cgtaaagcat tggttttgtg aggcccaggc cgccgacctg ttcgggatgg agcgagctgg
      121 ttattctcgg catcacaaaa acaaggaacg gcgtggcaag aaaagcgacg atcgagtatc
      181 ctgcagaaat ggccgctttt ttgtccctgt cttctatgag ccacctgagg agcagatagg
      241 cgatgtagat catcagaaga aagacaatcg atgtctccct tgggtcccag ttccatggcg
      301 aaccccaggt ataagtggca aaaaccgagc ctgtaatagt tgccagaaca caaaacacta
      361 gacctatctc ggagcttctg tgggcaacag agtcatatac agcttttctc ttcgcaagat
      421 aagctatcga gaagataaag gagatgaaaa atgcaagcgt tgcaacccac gcgcatggga
      481 tatggaagta aatgatcctg taaaggtctc ccattgttgc ttcttttggg gtaaacatga
      541 ggcaataaat aatcgaaggc accattgcaa ggggtgtgag caccgataaa atgcggccaa
      601 aaagcattgt ctattcctcc tcaatatacg gcaccaacac aagcccagcc accagtgccg
      661 ccagagattc actaattatc acaacaacaa gattaatcga aaatgtgcct tctgccaagg
      721 cttgtgaggt ggccaggaca aggcagacca gcaagggaag tattacaggc atcacgataa
      781 ctggataagc ggcgctcagg gcccttccgc gcgacatgag atagccgaca aaaccttgcg
      841 caaaagacaa ggcggggcac ccgatcaata ccagaaggcc aacaagcggc aaggctgaaa
      901 acctgacgcc aagccagaaa cacaaaagcg gaaaaacaac gcaggcaatc aacagaacaa
      961 aaaaagcatt cgcaagcgtt ttggcaagaa aaacggaagg catcggaacc ctgagcctca
     1021 actggtcggc cgttccctca tcctcctctt ttgcaaaaga cctggagagt gtgtcagaag
     1081 aggaaaacat gacaacaatc cagaggagcg aggctatgac cctttcgctg acctttgttc
     1141 cggcaagcga aaacccgagc gtggcggaga gaacaatcgc aagcccaaaa ccaaccccga
     1201 caaggtgcct ccccctgaaa gcgaccttta tttcttttgc cagaagtgcc ctaaaactgc
     1261 gcaatttttc tccagtctct ccagggattg tttgtggcaa ccacaacaag cctaccaaat
     1321 gacgagacca tgttgtccag aattgctttt ccctcggcat ccaggtgctg ctctggctca
     1381 tcaagcaata tgacgtcccg ggccattgca aaggctgtca tcagtttggc cctctgcttg
     1441 aggcctgccg aaagttcttg gtagcgcttt tcaaggtgac atgataaacc ccacacccca
     1501 aaatcaggca caggcacccc cgccaggttc gcaaaaaatc tggcattttc aaaaacagtc
     1561 atttccttgt aaagaccaag atcgggggaa cacatggcaa tcctgcctga caactgaaat
     1621 ttctcatact tcttctttct gtcaaaccac tccattgttc cagaagaagt tttgagcagg
     1681 cccgccatta tcctcaacag agtcgttttg cccgaaccat tcggcccaat gatcgcccat
     1741 acttcatctt ctatgtcaaa agaaacgcca gcaaaaacca tttttgaccc aaaagatttt
     1801 gacagattac tggcaaccat tcgcataacc aaagattata ggagtgttgc aaaatgaatc
     1861 aaaccagact aatctttttc tgttaaagtt gtcagttatt tcacaaaagg aggagtcatg
     1921 tcaaaaggct atatcttgag tgccgcaagg acacccattg gcaaattttt gggtagcctt
     1981 tcgccgtttt ccgcaccaaa acttggcggt ttcgccatca aggcggccgt tgaaagggcc
     2041 ggcgtagaaa aagagagaat tgacgaggta ataatgggcc aggtggtcca ggcaggctgc
     2101 ggccagaacc cggcaaggca ggcgcttatc catgcaggtc taaatcctga aacaaatgcc
     2161 ctcacaatca acaaggtgtg cggctcggga ctgaaagcga tcatgcttgc ttcacaatcc
     2221 atccagcttg gcgaggcaga cattgtcgtg gcaggcggaa tggaatccat gacaaatgca
     2281 ccatactacc ttgagaaggc aaggaccgaa ggctacagat atggagacgg cacccttgtc
     2341 gatggagtca taaaagatgg cctctggtgt gcattcaatg gttcccacat gggaaacctc
     2401 gcagaataca ctgcaaagaa gggtaacgtg accagggaag aggctgacct tttcgccttt
     2461 aattcccagg acagggcaat caaagccata gaaacaggaa tttttgacga agaagtagcg
     2521 ccaattgcaa taccacagaa aaaaggcgat ccgataatat tttcaaagga cgagaccccg
     2581 agaccaacta cgatggacac cctgcaaaag ctcaggccag cttttgacaa ggaaggggtc
     2641 gttactgcag gaaacgctcc tggcctctct gatggcggct cagcagttgt tgttgcatcc
     2701 gaaaatgtag ccaagaacca caaaccgctt gcttcaataa tcgattacgc aactgcacac
     2761 acagagccaa aagagctttt ctgggcacca gtctttgctg tcaaaaagct gatggaaaaa
     2821 acaggcctga aaatctcgga ctttgacctc atcgaagcca atgaagcctt tgctgtccag
     2881 tgcatagttg acggcagggg actcgaatgg gacccaaata cagtcaatat ctccggtggc
     2941 gccatagcac tcgggcaccc gataggttcc tccggttcaa ggattattgc aacactgatc
     3001 tacaacctga agaggaacgg caagaagcgc ggacttgcaa cactctgcct tggaggcgga
     3061 ggcgccgttg caatggcaat cgaacttgtg tgaggaggaa gcaatggaga taaaaaaagt
     3121 tggtgtcata ggcgcaggtg taatgggaaa cgggattgcc catgtatttg cacagaccgg
     3181 atacgacgtt gttcttgcag aagcaaacat ccagcttgct gaaaaaggca aggcaactat
     3241 ccagaaaaac ctcggcagga tggtccagaa aggcacaatc acacaggaag tcgcagatgc
     3301 aacgcttgcg agaataaaaa ccaccgaaaa ccttgaagac ctcagggatt gtcagatcgt
     3361 tgtcgaggct gtcactgaaa acaggcaggt caaaaaccag atatacgaca aattgaatgg
     3421 catcctgcag ggaaacgcaa ttctggcctc aaacacttcc acaatttcaa tcaccgagct
     3481 ggccgcaaaa tacaagaacc cagggaaatt tatcggcatg cacttcatga acccagttcc
     3541 cctcatgaaa cttgttgaag tgataagggg aatgctcacg accgatgaga cctacaatat
     3601 tgtgaatgat ctttcaatca aactgggaaa gacaccggca gttgcaaatg attatccagg
     3661 atttgtcagc aacaggatac tgatgccaat gataaacgag gcagtctact gcctcatgga
     3721 gggcgttgcc gacaaggaat caatagacac tgtaatgaaa ctaggcatga accatccaat
     3781 gggccccctc gcactcgcag accttatcgg ccttgatacc tgcctggcaa ttatggaagt
     3841 actccatgaa gggcttggcg actcaaaata caggccgtgc ccactgctga gaaaaatggt
     3901 tgcagcaggt ctccttggaa ggaaatcggg aaaaggtttc tacgactaca ccgaacaaaa
     3961 ataatcaacc acagaaactg acacactaga tcaagtttca acgccaacaa aaaagccccg
     4021 ctttttttaa agcggggctt ttttaattca tcagcgatga cagggggaat tgtttgctta
     4081 ctttaccctt atctttttgg tataagtgca agaattggca taacggtcag tagttctgag
     4141 ggttatgctg tagctacctg gggaataggt gtagctgaat tcttttgtgg tgtcattttc
     4201 gatattgaag gttttgtcaa tctttccatc accctcgaaa tcaagatcag ctgtgtatgg
     4261 gccacatcca ccaaatgggc tcactttgat ggtcattgtg ccagaggcag gtatgacata
     4321 aatgttgtca ttgtcaggtt ttgcttctgg aatcaagatt gaaatgtcgg gacactcaca
     4381 aaccactggt tcctttgcac caactctgag cactatgact gcagtatcct ggtctcttaa
     4441 accctctgcg gaggtgacaa tagcagtgtt tatgatattg atgccagggt taaggcttat
     4501 gtcctttctg actctcaatg tcagttcaat tatcttgact tcaccaggat tcatgattcc
     4561 aaatgaccag acaattttct gcatgccaag tgaccctgaa ggtattgagg ctacatattc
     4621 aagttctctt ggcaagatat caaccacaac aacattggtt gcttccctgg taccaatatt
     4681 ttttacaatt attgtgtagc taactttttc accctgctgg gccatgaact tctttgttgt
     4741 cttcgtgagc gtaatgagcg gttcgccaag aacatcaacg gtgcactgtg cgaattctgg
     4801 gtttggtgcg gtatcggcat acgtcagaac gttgttggtg agtttggtgc ctttggccaa
     4861 gtgaccaact cttactctca caacaagaac aaatgtctcg tgcggtgcaa gacttccgtt
     4921 tccagccaaa ccaacagaag tggtccatct gttgttgtca acatctggcg gcgggttggc
     4981 atcgtagaag gttactccct ttggatagtt ctcgacgata ttgacgtttg tctgtgtgta
     5041 tggggtgtcg tttgtgactg ttattgtgta aatcagaaga ctgtccggaa gaactgggtc
     5101 tgggacgcat ttctttgtta tctggagtga tggcacaacc gggacctgga gaatgaagcc
     5161 atgcatgtgg cccctctcat cagaacgtgg gtaatatggg tttggccaag gtccaccggc
     5221 tggcgaacca gcacagaaat tataattgtt ctctgttgtg gggtcacaat cagtataatc
     5281 attaccccat tctgtcgcca cacaaggccc ctgaccatca tccagcgaat tgggatcaga
     5341 tccaccagtg ttggcgcaaa ccagatatgg cctgagcagg actgatgaat cttcaccggc
     5401 aaggtacgtt cttgttggcc tgtgaagtgc ttttgtgtcg tagtcctggt ttacatagtc
     5461 ccaccattgg tcagctttcc agtggatggc tgtctttgcg gcaacaaccg tgaaattgcg
     5521 gtgccagaac catgagcagc tgtactgctt tgcattagaa gcatatcttg gattcattgc
     5581 tttcgggctc cacagatagc cagccgggaa gcctggtggc aatgttggag gggtttctgg
     5641 gttagcattc gaccctatcc agccatttgc ccaccatgcg ccgccctggc ctgctgttgg
     5701 gtcggccggt tgcggattga gccagaaacg gtctgttgga atcctgttgg tgccacatgc
     5761 ccagtaatcc cactctgccc tataccatcc aagttgtgag ccacaggttc cattatcagc
     5821 cgagtttcca attattccaa cataagcacc tgcatttgcg ccatcaacag gccatgtgga
     5881 aggatccggc ggtgtcctga aaccggagaa catggtttgc gaattttgcc tgtcattgtt
     5941 acaaagtgtc aaagcaccgt ctgcaatcat tatctgggag agtggaagag tggaattttc
     6001 aaaaatcatt actatttcaa aaccgtataa ttgaccattt ccaggctcat attcttgaac
     6061 cagatttgta acatgaatta tatcgtaagg atcagcgata tatggtgtaa tattgaaacg
     6121 gaatgttgac tgcatctggc cccagcccaa gcatccacaa cttggcctgt agccactgtc
     6181 gttgtttctt ccaaggaatg aaccggtaca aagaagagat tgcggattgc cttctctccc
     6241 cggatttaca agtattggta tatgctctcg gacttcatca ccagaatctc ggcgttgatc
     6301 aaaaattggt tggccaagcc agctaccatc aggatcttca attacccttc caactgagtt
     6361 ggcatacaga tagcacgcaa caagcctgga gttgtccggt atgtagtccg ggcgatattt
     6421 cagacgtgtt attccagcag ccccagggga cgaatcatgg acaaactgac aaaaatagtt
     6481 tttacgaatt tgtccttccc catcacgtgg atgtccaaaa tcggttgact gggcaatcat
     6541 tgcgaatcca tattttccaa cgaatggcgg gatgtcctga ttgagtctgt tgaatgatgt
     6601 tgtgaactgg tgaacatagt agaaccttcc agtgttcaca taatcatatt ccggatacgg
     6661 tgcaccagcg ccaaaatact ggtagccaag aaccacgcca tcagtgaaaa cgccttgcgt
     6721 tgctgttggc atattgttga atgatgcatc tccaccaaaa ccagcaagcg gggtaaacgg
     6781 ggagttgtca taagagaaag ctattctgtc tccaccgttt gttcctccac caatcataac
     6841 accgctatga atagaggggc caatctgctc acggcttgca aaataagctg tgatatttgc
     6901 tgttacaggt gggatattgg cagaagaggc atcaaaattt at
//
LOCUS       DAOSRI010000065         6938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10099_length_6938_cov_3.728171, whole genome shotgun sequence.
ACCESSION   DAOSRI010000065 DAOSRI010000000
VERSION     DAOSRI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10099_length_6938_cov_3.728171"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK717_02905"
     CDS             <1..763
                     /locus_tag="PK717_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR46644.1"
                     /translation="SQVKDLAIYYIGFQNKLEPFTNKKVRQAFNMAVNRQDIVDKIFK
                     GRHTLATGIIPPSMPNYKSLTDAYPYDPTKAKQLLEEAIKEGAKVPSKVVFAFNQGNV
                     THKNIAEFIQEQIKGNLGIDLQLESVEWATYLKRIDDGAFPMFRLGWVADYPDPDNFL
                     WVLLDSSNAGPKGGGAFYSNPNFDKLVRDAKKEKDNEKRMGLYAEAEKIAMEDACWMP
                     IVFQNSWTLVKPNVSGYFRNAMGPMMYNTVKIGPK"
     gene            840..1766
                     /locus_tag="PK717_02910"
     CDS             840..1766
                     /locus_tag="PK717_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46645.1"
                     /translation="MIQYALKRILYLIPVFIGVTFFTFLIFLVVPGDPVRLALGQHPN
                     PELQQKIEHELGMDLPWYIRYFRYIGKAVQGDLGTSIRTGEPVTKIILDRFPATLTLT
                     VAAMVFAIIVGLPAGIFSATKQYSWIDNTFMVMALVGISMPVFVLGLILLLIFVSFLH
                     IVPGTGYGDGQLIYMLLPAITLGTIPMAIISRMTRSSLLEVMGSDYIRTARAKGIKEK
                     SVILKHAFKNAFIPVVTVIGNNFASLMAGAIITERVFNWPGIGSATISAIEQRDLPVV
                     MGLVFFIAVIFVIVNLIVDISYIFIDPRIKLE"
     gene            1763..3097
                     /locus_tag="PK717_02915"
     CDS             1763..3097
                     /locus_tag="PK717_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46646.1"
                     /translation="MKNDTFVSATFKRLFRHKLALIGFCIIFLLVLVSIFADLMAPNP
                     NQMNLDAALTPPLKELYVARSKEKVPQNEYLYTVGPSDQKTLALMVDTGAGEKIKVEF
                     DKTGPALEAMAKDGTNKFVPQNDNILSPGDKVVGESTTISTFRSRKSVVVMKYKYRFA
                     TLSNLLVPSKEVIEGNLVAVDWVDEGSAFLVRDKKSGQLRLHKEGFADLSPTEEEIGK
                     MPKPLFGTDNAGRDISQRLAHGARISLYVGIIVELICLAIGIPLGAMAGFFGGRLDNF
                     IMRFTDIMFAFPGLLFAIAVMSIFENRDITTIFIALGLVGWPTIARIVRGQVISLKNT
                     EFVEGARAIGCSNLRIITNHIMPNIIAPIVVYTSLGIASAIMSEAGLSFLGIGIRPPT
                     PSWGTMINEGFQHALDAPHVWLMPGIVIALVVLAFNFLGDGLRDALDPKLKI"
     gene            3153..3644
                     /locus_tag="PK717_02920"
     CDS             3153..3644
                     /locus_tag="PK717_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOR46647.1"
                     /translation="MLVPLDIQHKTFKSAVSGYNKQEVHEFLAEVEKDFSQLYRENKE
                     LQEKLSKLTEEVSRYRATGTQLQQALTLAQKTADELKDSARRESELILADAKSKAEVV
                     TNESKQNVRVIKNAYLEFKGEFRAYLTTFLNLLDNMDSSLVGKIPVKDKEEEQLPQDQ
                     DNC"
     gene            3766..4227
                     /gene="lspA"
                     /locus_tag="PK717_02925"
     CDS             3766..4227
                     /gene="lspA"
                     /locus_tag="PK717_02925"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOR46648.1"
                     /translation="MKRFPLVEAIGFAVFLICDQITKIIVVHNLTYGASIPIIDGFLH
                     ITNTHNTGTIWGLAQGGNMIFAILAIVVVILIVVLTPKVATTKIARFAIGAILGGAIG
                     NLLDRFVRGFVVDFIDFRVINFPIFNVADIGIVCGAITLGIFMFLTPQKSN"
     gene            complement(4306..5433)
                     /locus_tag="PK717_02930"
     CDS             complement(4306..5433)
                     /locus_tag="PK717_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR46649.1"
                     /translation="MRRTFAFAVSLLVMIALSSCGISQGIKGKVTDKIYGDPVNVVTV
                     TLQNLDDMTLQPITATVNEKGEYEISVDPPGRYRVEIEDKRSNFQYVRFVRAVKIEQG
                     KVEQFDVQVDPVVKTYIHGQILAKDTGKPVEGAKIKFDSEEATTGKDGKYIFKYTKPG
                     PRVVTIEAKGYAIYKQSYNLSQGETMEDFKISPSAMADKPIIKNITDLLSYQVEVVVG
                     PNNETVSSSTKMVVNHLPFGLKVTSKNGTGLFYVTDSYIKKGDKFEKVSEEQFKIFRN
                     EYDSFNSLYNKVAQRISSGKDISTDKEPDIIGEFNAIRNTFKFEDSGKEYNTTMWIVY
                     EGPFLGLPAKVVLERQGEYIEFNFSLFNAIENSINKDLGNP"
     gene            5535..5610
                     /locus_tag="PK717_02935"
     tRNA            5535..5610
                     /locus_tag="PK717_02935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5568..5570,aa:Ala,seq:cgc)
     gene            5727..6179
                     /locus_tag="PK717_02940"
     CDS             5727..6179
                     /locus_tag="PK717_02940"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOR46650.1"
                     /translation="MEKTKIVLGKTKKIALVAHDNQKRALLEWAKLNREKLSRHQLFG
                     TGTTGRLVQQELGLEVHCFNSGPLGGDQQIGAKIVDGDIDLLIFFWDPLAAQPHDPDV
                     KALLRIAVLKNIPTACNRSTADFIFSSVLIDSEYEKVEPDYSSEIRRK"
     gene            complement(6189..6596)
                     /locus_tag="PK717_02945"
     CDS             complement(6189..6596)
                     /locus_tag="PK717_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOR46651.1"
                     /translation="MKKLYRSRKERVIGGVCGGIGVYFGIDPTLIRVIWVAALLMAGT
                     GLMAYIVAWLVIPEEPAGEETSVASRPRVECCKHFPVKLELVIGIFILALGIFLLLPN
                     LGFLSEYTWRFFWPALIILSGVMLMLLAVSKKR"
     gene            complement(6601..>6938)
                     /locus_tag="PK717_02950"
     CDS             complement(6601..>6938)
                     /locus_tag="PK717_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46652.1"
                     /translation="SLYLMNQGSVSFTDEHVHGTSDRLKMLGVSSGSFVAGKLFFFYL
                     ASIIQVIIFNAVCLLGFWVTEDVIAMNLFNPISNFFIYLLLWPLGMVLVAGLFYSLVS
                     NIKNATAAR"
BASE COUNT         1971 a         1433 c         1506 g         2028 t
ORIGIN      
        1 atcccaggta aaagaccttg ctatctatta cattggtttc cagaacaaac ttgaaccatt
       61 caccaacaaa aaagttcgcc aagcttttaa tatggccgtc aatcgccagg acattgtaga
      121 caaaatattc aaaggcaggc atacccttgc aacaggtatc atcccaccat ctatgcccaa
      181 ttacaaaagc cttacagatg cctatccata tgatccaaca aaagcaaaac aactcctaga
      241 agaagctata aaagagggag caaaagtgcc ttcaaaggtg gtttttgctt tcaatcaggg
      301 caacgtaaca cacaaaaata ttgctgaatt catccaggag cagatcaagg gtaaccttgg
      361 aatagacctt caacttgaat cagtggaatg ggcaacctat cttaagagga tagatgatgg
      421 cgctttccca atgttcaggc tcggttgggt tgcagattac cccgatccag acaatttcct
      481 ttgggtcctt cttgactcgt ctaatgcagg accaaagggt ggtggtgctt tctacagcaa
      541 ccctaatttt gacaaattgg tcagggatgc gaagaaagaa aaagacaatg aaaaacgcat
      601 ggggctttat gcggaagcag aaaagattgc catggaagat gcttgctgga tgccaatagt
      661 attccagaac agttggacac ttgtaaaacc caatgtgagc ggctatttcc gcaatgcaat
      721 gggcccaatg atgtacaaca cagtaaagat cggaccaaag tgataaaaag ctcaactatt
      781 atttcaatgg ggcgggttaa aacccgcccc attgaattat tgagccagga agtgcacaaa
      841 tgattcaata tgcactcaaa agaatccttt atttgatacc cgtctttatt ggagtgactt
      901 ttttcacatt tttgattttt cttgttgtgc cgggggatcc tgtaaggctt gcacttggtc
      961 aacatccaaa cccagagctc cagcaaaaaa ttgagcacga gcttggtatg gaccttcctt
     1021 ggtatataag atattttaga tacattggaa aagctgtcca aggtgatctc gggacatcaa
     1081 tcagaactgg cgaaccagtc acaaaaataa tattggacag attcccggcc acgcttactc
     1141 tcactgttgc agcaatggtt tttgccatta ttgttgggct tccagctggc attttttccg
     1201 caacaaagca atattcctgg attgataaca cttttatggt gatggcgctt gttggtattt
     1261 ccatgcctgt ttttgtgttg gggctaatat tgctcttaat ttttgtgtct ttcctccaca
     1321 ttgttcctgg cactggctat ggggacggcc aattgattta catgctcctt cctgcaataa
     1381 cccttggaac cataccgatg gccatcataa gtagaatgac aaggtcgtct ctcctggagg
     1441 tcatggggtc tgattacata agaactgcga gagcaaaggg aataaaagaa aagagtgtta
     1501 ttttaaaaca cgctttcaaa aatgctttca taccagtagt gacggtaatc gggaacaatt
     1561 ttgcgtctct tatggctgga gcaataataa ccgagagggt cttcaactgg ccaggaatag
     1621 gttctgcaac catcagtgcc atcgaacaga gagacttgcc ggttgtaatg gggcttgttt
     1681 tcttcatcgc cgttattttt gtaattgtca acttgatagt tgatatatcc tacattttca
     1741 tagacccaag gatcaagctt gaatgaaaaa tgataccttt gtttctgcaa cattcaaaag
     1801 attatttaga cataaacttg ccctgattgg cttctgcata atcttcttac tcgttctagt
     1861 ttcgattttt gctgatttaa tggccccaaa cccgaaccag atgaatcttg atgcggcact
     1921 taccccaccg cttaaggagc tttatgtggc caggtcaaaa gaaaaagtgc cacaaaatga
     1981 gtatttgtat accgtaggcc catcagacca aaaaactcta gcacttatgg ttgacactgg
     2041 agctggtgaa aaaataaaag tggagtttga taagacaggt ccagcccttg aagctatggc
     2101 aaaagatgga acaaacaagt ttgttccaca aaatgacaat attttatcac caggagacaa
     2161 ggttgttggt gaatctacaa ccatttcaac attcagaagc cggaaatctg tggttgtgat
     2221 gaagtacaaa tacaggtttg ctacactttc caaccttctt gttccttcaa aggaagtcat
     2281 cgaagggaac cttgtagctg ttgattgggt ggatgaagga tcagcttttc tagtcagaga
     2341 caagaaatct gggcaattga gacttcacaa ggaaggattt gcagaccttt ctccaaccga
     2401 agaagagatt ggtaaaatgc caaaacctct gtttggcacg gacaacgctg gtagagacat
     2461 ttctcagagg cttgcccatg gtgccaggat atcactttat gttggcataa tagtagagct
     2521 catctgcctg gcgattggta ttccccttgg tgcaatggca ggtttttttg gtggtcggct
     2581 tgataatttc atcatgcgtt ttacggacat catgtttgct ttccctggcc ttctttttgc
     2641 tatagccgtc atgtcaatat ttgagaatag agacatcacg acgattttta ttgcactcgg
     2701 tttggtagga tggccaacaa tcgccagaat tgtcagggga caggtcattt cacttaaaaa
     2761 tacggaattt gttgagggcg caagggcgat aggttgctcg aacctcagga tcataacaaa
     2821 ccatataatg ccaaatatta ttgccccaat agttgtatat acatcacttg gcattgccag
     2881 tgcgataatg tctgaagccg gcttatcttt tcttggaata gggataagac caccaactcc
     2941 gagttggggg accatgataa acgaagggtt tcaacatgcc ctcgatgcac cacatgtatg
     3001 gttgatgccg ggtattgtaa ttgcgcttgt tgtacttgct ttcaacttcc ttggcgatgg
     3061 gttgcgtgat gcacttgacc cgaagctgaa aatctaggct tgacagaact gtcatcacag
     3121 gttaaatttc ttgaaagaat gaggaggcca aaatgctagt acctcttgat atacaacata
     3181 agacgttcaa gtcggctgtt tctggctaca acaagcagga agttcatgaa ttccttgctg
     3241 aagttgaaaa ggacttttcc cagctctaca gggaaaacaa ggagttgcag gagaagcttt
     3301 cgaagctcac cgaagaagtc tctagataca gagcaacagg cactcaactc caacaggccc
     3361 ttacgcttgc ccaaaaaacg gccgatgagc tgaaggattc agcacgcaga gaatccgaac
     3421 tcattttggc tgatgccaag agtaaagctg aagttgtcac caatgagtcc aagcagaatg
     3481 tgagggtcat aaagaatgcc tatcttgaat ttaagggaga attcagggct tatttgacca
     3541 cattcctgaa cttgcttgac aatatggaca gctcacttgt aggcaaaatt ccggtcaagg
     3601 ataaggaaga agaacaactg ccgcaagatc aggacaactg ctgatccctg tttggttatt
     3661 ttgacagtta acatttaaaa tttattgtgc ccggcgtttt ttgccgggca catatttttg
     3721 gaaaccagat agatactctt tctacacatg gatgcaggag gttgaatgaa gcgttttcca
     3781 cttgttgagg ccattggctt tgcggttttt cttatttgtg accaaattac caaaataatt
     3841 gttgtccaca atttaacata tggtgcctca atccctatca ttgacggatt tttgcatatc
     3901 accaatactc ataatactgg aactatatgg gggcttgcgc agggaggaaa tatgattttt
     3961 gcaatccttg cgatcgtcgt tgtcatcttg attgttgtgc tgacaccgaa agtggcaaca
     4021 acaaagattg cccgttttgc gattggtgct attttgggtg gtgctattgg aaatctcctg
     4081 gacaggtttg tgagaggttt tgttgttgat tttattgatt ttagagtgat aaacttccct
     4141 attttcaatg tagccgacat tggtattgtt tgtggcgcaa taacacttgg gatatttatg
     4201 ttcctaacgc cacaaaagag caactgaaat aaacgtagaa aataaaaaaa ccggacgatt
     4261 gctgtccggt ttttttattt gtttatagta acgattgcag attgatcaag gatttcccaa
     4321 atctttgttt attgagttct caattgcgtt gaacagtgag aaattaaatt caatgtactc
     4381 tccttgtctt tcgagtacaa cctttgcagg aagcccaagg aatgggcctt cgtaaacaat
     4441 ccacattgtt gtgttgtatt ctttgcccga atcttcaaat ttgaaggtgt tacggatggc
     4501 attgaattcg ccaattatgt ccggttcttt gtcggtgctg atatcttttc cactggaaat
     4561 gcgctgggcc accttgttat aaagagaatt gaaggagtca tattcatttc taaaaatctt
     4621 gaattgttct tctgaaacct tctcaaattt atcgcctttt ttgatatatg agtcagtcac
     4681 atagaacaat cctgtgccat ttttgcttgt gactttcaaa ccaaatggca agtggtttac
     4741 gaccattttg gttgatgagg acacggtttc attgtttggt ccaactacta cttcaacctg
     4801 atatgatagc aagtctgtga tatttttgat tattggtttg tcggccatgg ctgatggtga
     4861 aatcttgaaa tcttccatag tttcaccctg tgagaggtta tagctttgtt tgtagatggc
     4921 gtaacctttg gcttctattg tcacaactct tggacctggc ttggtgtatt tgaatatgta
     4981 ttttccgtct tttccggttg ttgcctcttc ggagtcgaat ttgattttgg cgccctcaac
     5041 cggtttccct gtgtctttgg caaggatttg accgtgaatg taggttttaa caacaggatc
     5101 cacctgcaca tcaaattgtt cgactttacc ttgctctatt tttactgcgc gtacaaacct
     5161 tacgtattgg aagttgcttc tcttgtcttc aatctcgacc ctgtatcttc cgggtggatc
     5221 aactgaaatc tcatattcgc ccttctcgtt taccgtggct gtgattggct gtaatgtcat
     5281 gtcatcgaga ttttgcagtg ttactgtgac cacattcact ggatcgccat atattttatc
     5341 ggtcaccttt cccttaattc cctgtgaaat gccacatgac gaaagagcta tcatcaccag
     5401 gagtgaaaca gcaaaagcaa atgttcttct catacttacc tccatacaaa ttttattgtc
     5461 ttgccgttcc tcaatcaagc tttttaagtg tttgttgaat caatccaagc tattgcataa
     5521 actggtgtat gagtggggca gtggctcagt tggcagagcg catccttcgc aaggatgagg
     5581 ccgggagttc gaatctcctc tgctccacca tatataaaaa tgattattaa caaaatttct
     5641 aatccaactt ccctaacaat tttatagcat ttgtttgtat aattgacttg atagaataaa
     5701 cgagccattc acttgttgga gagataatgg aaaaaacaaa gatagtattg ggaaaaacca
     5761 agaaaatagc gcttgtcgca catgataatc agaaacgggc cttgctcgag tgggccaagc
     5821 tgaataggga aaaactttcc agacaccaac tttttggcac aggcacaacc ggccgtctgg
     5881 tgcagcaaga actgggtctt gaggtgcatt gcttcaactc tggtcctttg ggtggcgacc
     5941 agcagatcgg cgctaaaata gtcgatggtg acattgacct tttaatattc ttctgggacc
     6001 cgcttgccgc acagccacat gatcctgatg tgaaggccct tttgaggatt gcagtattga
     6061 agaacatacc gacagcatgc aacaggtcta cggccgattt cattttctct tcagtgctta
     6121 ttgacagcga gtatgaaaag gtggagccag actattcttc agaaattcgt agaaaataaa
     6181 ttttcttgct atctcttttt tgaaacagca agcaacatta gcatgactcc ggaaagaatt
     6241 attagtgccg gccagaaaaa tctccatgta tactcgctaa gaaagccaag atttggaagt
     6301 agcaggaata tcccaagggc gagtatgaag atgccaataa ccagctcgag ttttaccggg
     6361 aaatgtttgc aacattcaac tctaggccga cttgcaactg atgtttcttc tccggctggt
     6421 tcttccggta ttacaagcca ggcaacgatg tatgccatca aaccggtacc tgccatgagc
     6481 agggccgcca cccagattac ccttatcagc gtcgggtcta tgccaaaata aaccccaata
     6541 cctccacaaa caccgccaat caccctttct ttccttgagc gataaagttt tttcatgcgg
     6601 tcatcttgcg gctgtggcgt tttttatgtt tgaaacaaga gagtagaaca atcctgcgac
     6661 aagcaccatt ccaagtggcc agagcagcag gtatatgaag aaattggaga ttgggttaaa
     6721 gaggttcatt gcaattacat cctctgtaac ccaaaatccc aagagacata cagcattgaa
     6781 tataattacc tgaataattg atgcgaggta gaagaagaag agtttgccgg ccacaaatga
     6841 gccagacgag acaccaagca tttttaatct atcagaagtc ccgtgaacat gctcatcagt
     6901 aaaagaaaca ctgccttggt tcatgagata gagtgacg
//
LOCUS       DAOSRI010000066         6821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10327_length_6821_cov_3.207508, whole genome shotgun sequence.
ACCESSION   DAOSRI010000066 DAOSRI010000000
VERSION     DAOSRI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_6821_cov_3.207508"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK717_02955"
     CDS             complement(<1..703)
                     /locus_tag="PK717_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46653.1"
                     /translation="MKSVPRALRVVFLAVGILLVIMIFLSFFPPLVPVSRVNIDKTER
                     IPIPKVVDYLETKLVMGLVPTTNSIFDDAKDTLGPLLEDVTIKRHFFFSATVTIKEKK
                     ETYNLKMNAGKVAVDVEGRIVDSIRVSEDAPVEMEVAYLQPGGYGMLDASFYNDKMAP
                     VVAQILKYKKLLPVKIKTLFYNKSEYVTSSGSRGGVFLAPVSYPDGIAKAMFDGKYKR
                     AYLESMQGFYAETSQG"
     gene            complement(1010..2545)
                     /gene="ftcD"
                     /locus_tag="PK717_02960"
     CDS             complement(1010..2545)
                     /gene="ftcD"
                     /locus_tag="PK717_02960"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOR46654.1"
                     /translation="MARLVECVPNFSEGRRKDVVEKIAQVAKDTPNVKLLDVQMDADH
                     NRCVVTFVGEPEAAAEAAFGMCKVASELIDMNTHKGEHPRLGATDVIPFVPISEVTTE
                     EAVKLAVSLGERIWKELKIPVYLYEDAARTPERQDLSNIRQGEYEGIKKEMGEKPERN
                     PDIGEPAMHPTAGAVVVGARMPLVAFNVNLGSQNIEVAKEIAKLIRFRSGGLRFVKAL
                     GFSIRERGFVQVSMNLTNYKKTSIRTAYEFVKREAERLGVLPIGAEIVGLLPLDALVD
                     VGVSYMNVWDFKKAQIIDLRVSASDSGEVSWIPKNFIREVASKSPAPGGGSVAASAGA
                     LGAGLSAMVAKLSQGKKYAEVKEEMEKVAAKADEVTEKLLSLIQEDSDAFNEMLACRK
                     LPSGTPESDAFRQKKMQEAIAKASLVPLNTMRTAAMAVPLIKTVAQKGNPNSVSDAGV
                     AALSIKTAIYGAHMNVMINLPSLEDKEMASKMMAEADKIEKEAIPQCDEIHKFVLEKI
                     KQM"
     gene            complement(2533..4338)
                     /gene="hutI"
                     /locus_tag="PK717_02965"
     CDS             complement(2533..4338)
                     /gene="hutI"
                     /locus_tag="PK717_02965"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOR46655.1"
                     /translation="MADKQKKVIKNLFYGFLVLVVIAAVGTTVFFLTRLTPKKDNLFS
                     DPNGFFEMELPEDWVYYKGADVVKFDSSLGHASYSFVFYPKGQNMEIEKFTGNVLTCA
                     VFLKEWVGDKEHYDKFFGYMGGINNPKKYMTEEILQKGEKIPGVKDGGYTFEKGWPLA
                     TVKSKNAMIFFNGSYSGDEEKNNRKNASFNKNTMKADLILRNCSELVTLAGPCPRKGN
                     QMDNLAIVKNGSFAIKDGLFVAVGTESEIVSNYESETIIDASGCCVLPGFVDSHTHII
                     FAGDRSAEFEMRLQGATYEEIMAKGGGILNTVSTTRLASYEELYAQSAKRLKTMIKTG
                     TTAFEMKSGYGLEANAEMKMLMVAKKLIHDFGVEAKTTFMGAHAIPKDIDRNDFISLV
                     KGEMLYVCKPYSDFIDVFCDKGAFTNEETEDIVKDSGLPVRLHVDELADTGGASLAAK
                     LGAVSADHLIGASNKGFEDMARSGTVATFLPGTSFFLGKPFARAKKAIESGCVVAIAS
                     DRNPGSCTIQSMPFVVGLACLRLGMTPAQAISAATINAAYSIRSHDRVGSIEKGKRAD
                     FVIYDVESYRMIPYELGVDHAKMTFIKGKEASWPD"
     gene            complement(4391..5833)
                     /locus_tag="PK717_02970"
     CDS             complement(4391..5833)
                     /locus_tag="PK717_02970"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOR46656.1"
                     /translation="MAVWQGKTNRLDQNRLEIPGTHKAFMRVPGIIFCSVPMEQGLFA
                     DNAPEQVANVASLPGIIKASFAMPDIHWGYGFPIGGVAAFDEKEGVISPGGVGYDINC
                     GVRLLRSNLVAHDVVKHVEKIIGSIYSNVPSGVGSEGKIRLERQELQKVLVEGSAWAV
                     KKGYGYQEDLQFIEDGGQMAGANPDAVSKEAVKRGTPQLGTLGAGNHFLEIQEVTEIY
                     DEKIAGTFGLFVGQMVVLIHTGSRGLGHQVATDYIDVMQNAAKMYGIELPDRQLACAP
                     FMSPEGQRYFGAMAASANYAWANRQMITHWVRESFERSLGMSQEKLGLGIVYDIAHNI
                     AKRETHLGRKVVVHRKGATRAFPAGFPGIPETYSETGHPVLVPGDMGRYSFVMVGTKK
                     GAEEVFASCCHGAGRVLSRTQAIKNYRPGQVIEKLRQNGTYLKAANSETISEEAPGAY
                     KDVAMVVDVLDGAGIAKKIAKMRPLGVVKG"
     gene            complement(5836..6210)
                     /locus_tag="PK717_02975"
     CDS             complement(5836..6210)
                     /locus_tag="PK717_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOR46657.1"
                     /translation="MFGKPTSEVRDRSESYISSTLKITGTIYCNGILRIDGTLEGNVE
                     GEGHLIVGRTAHIKGDIRMKSVTNLGTIEGNLFCTDVIELLEGSKLLGDVNLETSRFR
                     IEENVTFSGRSKTNLKTLAKQQ"
     gene            complement(6225..>6821)
                     /locus_tag="PK717_02980"
     CDS             complement(6225..>6821)
                     /locus_tag="PK717_02980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR46658.1"
                     /translation="KASVAVITPNNTLEQVIAENSLKQESVPKPAAYLPVTYDNLQKT
                     SEHVIKESDERTRALSVMNAYAQEYKDLRDRTPTGWPLQGRLTSFFGWREWTGTFHEG
                     IDIVAPLGTPLHATASGTVIRAEWFSGYGLCVDVLHRDGIVTRYGHCDKILIKKGDGI
                     KVGQVIALTGSTGNSDIPHCHYEIRINDKSVDPMKFPF"
BASE COUNT         1715 a         1724 c         1432 g         1950 t
ORIGIN      
        1 tgccctggga ggtttcagca taaaaacctt gcatgctctc cagatacgct ctcttgtatt
       61 tcccatcaaa catcgctttt gcaataccgt ccggatagct tacaggagcg aggaaaacac
      121 caccccttga tccggatgat gtaacatatt ctgatttatt gtagaacaaa gttttgattt
      181 ttacaggcaa caatttctta tatttaagaa tctgcgcaac cactggggcc atcttgtcgt
      241 tataaaagct tgcatcaagc attccataac cacctggttg caggtatgcc acttccatct
      301 cgacaggtgc gtcctcagaa actcttatag aatcgactat tcttccttcg acatcaacag
      361 ccaccttgcc cgcattcatc ttcaaattgt aagtttcctt cttttccttg attgtcaccg
      421 tagcagaaaa gaaaaaatgc ctttttatag tcacgtcttc aagaagtggg ccaagggtat
      481 cttttgcgtc atcaaatatt gaatttgttg ttgggacaag ccccattaca agttttgttt
      541 ccagatagtc aactactttt ggtattggta ttctttcggt tttgtcaata ttgactcttg
      601 agacaggaac caaaggaggg aaaaacgata agaaaatcat tatcactaat aaaatcccca
      661 cagccaagaa aacaactcta agagctctcg ggaccgattt catggttcct tactatacac
      721 aattcatcct ttttgtcaat tagtctgact tcggattggc ttgacttttt aggagcaagt
      781 cgggcaaacc ccgcggagaa ggacaaaaaa acggcatgat aaaatactgt ataaatcaat
      841 attaagtcca tgttgcaaaa aagaaatcgg caggttcgac gtcactgttt taacttccac
      901 atccggaaga tcaccaaatc acgaaaaata aaaatgccgg tttagtctat atttcattgg
      961 caaaccggca ttttttggta agcgtaaata taaaatgtta gttttttggt tacatttgct
     1021 ttattttttc gagcacaaat ttgtggatct catcacactg ggggatcgct tccttttcaa
     1081 tcttgtcagc ttctgccatc atttttgatg ccatctcctt gtcttcgagg gaaggcaggt
     1141 tgatcatgac attcatgtgt gcaccataaa ttgcagtttt aatagataag gcggcaacac
     1201 cggcatcaga aacggaattc gggtttccct tctgtgcaac cgtcttaata agaggaactg
     1261 ccattgcagc ggtcctcatc gtgttgagag gaacaaggct cgcttttgca attgcttcct
     1321 gcatcttttt ctgcctgaaa gcgtctgact cgggagtgcc ggatggaagc tttctacatg
     1381 caagcatttc gttgaatgcg tcagaatcct cctggatgag cgacaggagc ttttcggtaa
     1441 cctcgtctgc cttcgcggca actttttcca tctcttcttt tacttcggca tactttttgc
     1501 cctgcgacag cttggccacc attgcggaaa gcccagcacc aagcgcaccg gctgaagcgg
     1561 caacagagcc gccgcctggg gctggtgatt tacttgcaac ttccctgatg aaattctttg
     1621 gtatccagga aacctccccg gaatccgaag cgctgaccct gaggtcaatt atctgcgcct
     1681 tcttgaagtc ccagacgttc atgtatgaaa caccaacatc aacaagtgcg tcaagcggca
     1741 ggagaccaac tatttcagca cctatcggga gcacacccag tctttcagcc tctcttttga
     1801 caaactcgta ggcggtcctt atcgaggttt tcttgtagtt tgtaagattc atcgaaacct
     1861 ggacaaagcc cctctctctg atcgagaaac caagggcttt gacaaacctg agaccaccgc
     1921 tcctgaatct gatgagcttt gcaatctcct tcgcaacctc gatattctga gagccaaggt
     1981 tcacgttgaa ggcaacaagg ggcatcctgg cgccaacaac aactgctccg gctgtcggat
     2041 gcattgcagg ttcgcctatg tcagggtttc tttcaggctt ctcgcccatt tccttcttaa
     2101 tgccttcata ttcgccctgc ctgatgtttg ataggtcctg gcgctcagga gttcttgcag
     2161 catcttcgta tagatagacc gggattttca gttctttcca tattctctcg ccaagcgata
     2221 ccgccagctt gaccgcctct tcggtagtga cttcagaaat cgggacaaac gggatgacat
     2281 ccgttgcgcc aaggcgcggg tgttcgccct tgtgtgtgtt catgtcaatt agctctgatg
     2341 caaccttgca catcccgaat gctgcctcag cggccgcctc cggctccccg acgaatgtca
     2401 caacacacct gttgtgatca gcgtccatct gcacatcaag cagcttgacg ttcggggtgt
     2461 cctttgcaac ctgggctatc ttttctacaa catcttttct tctgccttca gagaagtttg
     2521 gtacgcattc aactagtctg gccatgatgc ctccttgcct ttgatgaatg tcatttttgc
     2581 gtggtccacg ccaagttcat agggtatcat tctgtaagat tcaacatcat aaatgacaaa
     2641 atcggctctc ttgccttttt ctatagagcc aaccctgtcg tgcgatctga tggaatatgc
     2701 ggcattgatt gtggcagcag atattgcttg tgctggcgtc atgcccagcc tcaagcatgc
     2761 cagtccaact acaaacggca ttgactggat cgtgcaggaa cctgggtttc tatcagaggc
     2821 aatggcaaca acacagcctg actcaatagc cttttttgcc ctggcaaaag gtttgcccag
     2881 gaagaaagat gttcctggga ggaaagttgc aacagttcct gatctggcca tgtcctcaaa
     2941 acctttgtta gaagcgccga tcaggtggtc tgcagagaca gcaccaagtt ttgcggcaag
     3001 tgaagcacca ccagtgtcag caagctcatc aacatggagc ctcaccggca agccagaatc
     3061 ttttacaata tcctctgttt cctcgttcgt gaatgcccct ttatcgcaga acacatcgat
     3121 gaaatcggaa tagggtttgc acacatagag catttctcct ttgacgaggg aaataaaatc
     3181 attcctgtcg atatctttgg gtatggcatg ggcgcccatg aaggtcgttt tggcctcgac
     3241 accaaaatca tggatgagtt tttttgcaac catgagcatt ttcatctctg cattggcctc
     3301 aagcccgtaa cctgacttca tctcaaaagc cgtcgttccg gttttgatca ttgtcttcag
     3361 gcgctttgcc gactgggcat aaagctcctc ataagaggcc aatctggtgg tacttacagt
     3421 attgagtatg ccgccaccct ttgccattat ctcctcgtag gtcgcgccct ggagcctcat
     3481 ttcgaactcg gccgacctgt caccggcaaa aataatgtgg gtatgagaat ccacaaaacc
     3541 aggcagaaca caacacccac tggcgtcaat aatggtttct gattcgtaat ttgaaacaat
     3601 ctcgctttct gtcccaacag caacaaaaag gccatcctta atagcaaaag acccgttttt
     3661 aacgatcgcc agattgtcca tctggttgcc ctttcgggga cacgggccag ctagggtcac
     3721 aagctcagaa caattgcgaa gtatcagatc agctttcatg gtgtttttat tgaaagaagc
     3781 attttttcta ttgttttttt cttcatcccc agagtaagat ccattgaaga atatcattgc
     3841 attctttgac tttacagttg ccagtggcca acctttctca aaggtatacc ctccatcttt
     3901 tacccctgga atcttttcac ctttctgcaa aatttcctcg gtcatatact tctttgggtt
     3961 attgatgcca cccatgtaac caaagaactt gtcgtaatgt tccttgtctc caacccactc
     4021 tttcaaaaaa accgcacagg tgaggacatt accagtaaat ttctctatct ccatgttctg
     4081 gccttttggg taaaaaacga aggaatagct tgcatgacca agtgaactgt caaatttgac
     4141 aacatcagcg cctttataat atacccagtc ttctggcagt tccatttcga aaaaaccatt
     4201 tggatcgcta aacagattat cttttttggg ggtgagcctc gttagaaaaa aaacagtcgt
     4261 gcccaccgca gcaatgacaa caagcacaag aaaaccataa aacagatttt ttataacttt
     4321 tttctgtttg tctgccatgc ctctgcggga ccttattctt gataaacggt ttctgttcga
     4381 catcctattt ttaccccttc acaacaccaa gtggtctcat ttttgcaatc tttttggcaa
     4441 ttcctgcacc atccaagaca tcaaccacca tggcaacatc tttgtatgca ccaggggctt
     4501 cttcactaat agtttcagaa tttgctgctt ttagatatgt cccgttctgc ctaagtttct
     4561 caataacttg tccgggtctg taatttttga ttgcctgtgt tctggaaagg acccttcccg
     4621 caccatgaca gcaagatgca aaaacctctt cagcgccttt tttggtacca accatgacaa
     4681 aagaatatcg acccatatca cctggaacaa gtactggatg accagtttcg ctgtatgtct
     4741 cgggaatccc agggaaaccc gcagggaagg cccttgtagc acccttgcga tggacaacca
     4801 cttttctccc aaggtgtgtt tccctctttg cgatattgtg ggcaatgtca tagacaatcc
     4861 ccaaaccgag tttctcctga ctcatcccaa gagacctctc aaaactttcc ctgacccagt
     4921 gggttatcat ctgcctgttt gcccaggcat aatttgctga tgcagccatg gcaccaaaat
     4981 agcgctggcc ttcgggagac atgaacggtg cacatgcaag ttgcctgtct ggaagctcga
     5041 tgccatacat ttttgcagca ttttgcatga catctatgta gtcagttgcg acctggtgcc
     5101 cgaggcccct cgacccagtg tgtattaata cgaccatctg gccgacaaaa agcccaaatg
     5161 ttcctgctat tttttcgtca taaatctcgg tgacttcctg gatttccaga aaatggttgc
     5221 cagcacccag agttccgagt tgcggtgtac ccctctttac tgcttctttc gagactgcat
     5281 cgggatttgc accagccatt tgcccgccat cttcaatgaa ttgcaggtct tcttgatagc
     5341 cataaccttt tttgaccgcc caggcagaac cttcaacaag tactttttgg agttcctgcc
     5401 gttccagcct tatttttcct tcggagccca caccagatgg aacattgcta taaatcgagc
     5461 caattatttt ctcgacgtgt tttacgacat cgtgggccac caggttggaa cgaaggagcc
     5521 tgacaccaca gttgatatca tatccaacac caccagggga gatgacacct tctttttcat
     5581 caaacgcggc cacgccacca atcgggaaac catagcccca atgaatatca ggcatggcaa
     5641 atgatgcctt gatgatacca ggcaaagaag caacattggc gacttgttct ggtgcattgt
     5701 ctgcaaaaag gccttgctcc atggggacag aacaaaagat aatccccggc accctcataa
     5761 aagctttgtg agtgccgggg atttcaagcc ggttttggtc cagcctgttt gtttttcctt
     5821 gccaaacagc cattgctact gctgttttgc cagtgtttta aggtttgttt ttgacctgcc
     5881 ggagaaggtg acattttcct caatcctgaa acgggacgtt tcgagattga cgtcacccag
     5941 cagttttgaa ccctcgagga gctcaatgac atccgtgcag aagaggttgc cctctatggt
     6001 gccaaggttg gtcacagatt tcattctgat gtcacccttg atatgggcag tacggccaac
     6061 aatgagatgg ccttcaccct caacattgcc ctcaagtgtg ccatctattc ttagaatgcc
     6121 attgcaataa attgtgcctg tgatcttcag cgttgaggat atataggact cagaacggtc
     6181 cctcacctct gatgttggtt ttccaaacat attttttgtg actcctagaa cgggaacttc
     6241 atcggatcta ccgatttgtc gttgattctt atttcgtaat ggcaatgtgg tatgtcggaa
     6301 ttaccggttg aacctgtcaa cgcaatgacc tgaccaactt ttatgccatc acccttcttg
     6361 atcagtattt tatcgcaatg gccgtagcgg gtaacaatgc catcacggtg gagaacatca
     6421 acacagagac cgtagccaga gaaccattca gctcttatta cagttccaga ggcggtagca
     6481 tgaagtggtg ttccaagcgg ggcaacaatg tctattccct catggaaggt accagtccac
     6541 tctctccagc caaaaaaaga ggtcagtctt ccctgaagcg gccatccagt tggtgtacga
     6601 tcccttaggt ccttgtattc ctgggcatag gcattcatta cagaaagagc ccttgttctc
     6661 tcatcactct ctttaatgac atgttccgag gttttctgga gattgtcgta agtgacaggc
     6721 aaataagcgg caggcttcgg aacagactct tgcttgaggc tattttctgc aatcacttgc
     6781 tcgagagtgt tatttggggt tataaccgca acagaagcct t
//
LOCUS       DAOSRI010000067         6816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10334_length_6816_cov_3.710250, whole genome shotgun sequence.
ACCESSION   DAOSRI010000067 DAOSRI010000000
VERSION     DAOSRI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10334_length_6816_cov_3.710250"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1038
                     /locus_tag="PK717_02985"
     CDS             238..1038
                     /locus_tag="PK717_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR46659.1"
                     /translation="MRNIIEVTGLTKLFGNLVAVNNLSLEVLEGESFGLLGPNGAGKT
                     TTIKMLTTLLLPTSGSAKIGGYDVVKNAYEVRKIIGYVPQLISTDPMLTGLENLTVFA
                     KLYDVPARTRKSQINEAFEFMGLMDAKNKLVKNYSGGMVRRLEIAQSLLHKPKVLFLD
                     EPTVGLDPVARKMMWEHIAKLKTEFGTTIFLTTHYMEEADRLCDRVAIMDRGKMSATG
                     TPDSLKDSLGIEGATLDDVFVRYTGNALDEGAGFKDTRRTRRVAKRLG"
     gene            1043..1831
                     /locus_tag="PK717_02990"
     CDS             1043..1831
                     /locus_tag="PK717_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46660.1"
                     /translation="MTILQTISNFIKKTFVIVELELKKLKHDPSEIITRAIQPTLWLM
                     VFGQVFTRTRAIPTGDIPYIDFMAPGILCQSVLFISIFFGITIIWERDLGIVHKFLVS
                     PAPREALVLGKALSAGFRGLSQAVIIFLLSLLLGVGVSWSPLSILGVLFAIVIGSALF
                     STLSLIIACLVKTRERLMGIGQLITMPLFFASNAIYPIQMMPEWLKYIAWGNPLTYLV
                     DATRSMMLVKYQSQFGLTLDFAILLAVLIVFIFIGGRLYPKVVN"
     gene            1899..5480
                     /locus_tag="PK717_02995"
     CDS             1899..5480
                     /locus_tag="PK717_02995"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR46661.1"
                     /translation="MITGGRYELLKKLGEGGMGEVYMAFDTHTKSHVAIKFAFLGSRD
                     PESEKKRFFREIELLKSIDHPNIVKLLDSGQDGENLYYVMEYIQGHEIRERENFKRLA
                     GLLAIVANALFALHQKGIVHGDIKPSNIMLVENRPVLLDFGVAIANMQGQLRKGGEVS
                     GTVGYMSPEGVMGQKTDGCSDLYSLGAVLYEQLTGCLPFGEGQVQNQIFRILHEKPIR
                     PSLVNPRVPEELEEICLKLINKDPGSRYQSGKEVEDALLSFSRGVTEEKSARSAISTV
                     KVPLVGREEEVSKFSDCLDLLMLGEGRIVLVQGESGIGKTKLVEEFRTVALSRKVKFI
                     LCDGKQAWPGAPAIGRILDELAGYDIMVDRPLISRFSKQIELLSPLFFKRHNLDFSAY
                     IDDGREQFSAALAKILLYAFDEGIVFAFDGITDPQTIDVCKEMVIAAPKAKKLLVFSG
                     FDPESIQPQNALALPVVLDLKPLGRTGIKKLADYLKLSLSDDLADEVLRFSKGIPKNA
                     IEILRQLAINPSALNDGMKDMKNSVSARRFAGVGRNAREMLGVVCLLDRPLPSRMICA
                     IARLPEDQLGGTIDELVSKGFVRETLLDGELVVEPILPEVVQFVRGHVPARELTELHR
                     RIAEHLEKTLNEGSKAEYRKEVGSHYIEAGEYLRAAKYLMPVLREMQSKYQFALCNDI
                     INKLLPHLNRITDREVALSFSLSCVAQFVNTGITPFLKDTSEMLEQALLEENTLENMK
                     IKYATWLGLARARLGEFNNALARFELAWSFFIVAPNDEDGFYLTRAFSEVYINSSQFE
                     KAKKYCLLNIKYAKKLKNDARLFQSFNNLGIVQMMMGKNDEAEEIFKKNIALSRKVGS
                     QLQEVTSLLNLVGVYSNRKEFDKTISLLLDLKDKAKDLGDINKLSIIYINLINTYEKI
                     YRKEEALSVLEEALSQLNKLSSTFRLHTLYEKAVRIALEMENFDLAEHFIEDLQSRAF
                     ESGNKFQEAFAFALQGDMMFIKGNHREAIVNLKIAMNMFEKAKQEHKGIYCMIAHKYA
                     FCVASEGNINEARKILKITNPMLKEILDKEIVGFSITFEAWVDMIESFSGNVEQKGSG
                     KEDSTFSKDLWNRSYRKYRKVMDPLLGVDECMLPRPYAAMGFTRLVLDRGRTDPKYKE
                     MFQEDCKRAILYLDEAIMQCQKSKTRLYTGVLIGLREKTRDAASQAS"
     gene            complement(5431..5928)
                     /locus_tag="PK717_03000"
     CDS             complement(5431..5928)
                     /locus_tag="PK717_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOR46662.1"
                     /translation="MDAFKPKAYFKRVSQIPFSLLMSWGIKAVFLDTDNTLAPRDSDA
                     FVKDALAWVEACKRAGFLLCIVSNGRPGRVRRASEKLGIPFVNPALKPLSYGFKKAAR
                     MMRVHSKQCVMVGDQIFTDIWGGNNAGMKTILVEPVDPKSDGLWTKWIRKLEKRHLGF
                     SPEGL"
     gene            complement(5915..6130)
                     /locus_tag="PK717_03005"
     CDS             complement(5915..6130)
                     /locus_tag="PK717_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46663.1"
                     /translation="MVTYELIIDEPDIWFVQSVLDTADNICIVDKTNMVGPKGHMKVM
                     FSEEAEKDFLYLVEKLWQGSSFTYGRI"
     gene            complement(6124..6534)
                     /gene="tsaE"
                     /locus_tag="PK717_03010"
     CDS             complement(6124..6534)
                     /gene="tsaE"
                     /locus_tag="PK717_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR46664.1"
                     /translation="MNKTELVVPSQEELIALGRWWGEGMEGNEVYALKGPLGAGKTTL
                     VKGIALGLGITDNIVSPTFVLVMEYKGRHTLYHFDWYRIETEKEVVDLGYYDYLEKPG
                     VKVIEWPDKFIDLIPENAKWITIEVDGSNRIVRW"
BASE COUNT         1913 a         1410 c         1629 g         1864 t
ORIGIN      
        1 agaggcgatg tgggaaagat ggtggccttg aaaggtggac taattgtccc ggccgacatg
       61 gacgaggcaa ccagagaacg ccgtaaacca gaagccaaac ttttcgaata cgcaagactg
      121 ttctactagg gcatgcttta tggccccggg tggatttgcc cggggctttt ttgtttcttt
      181 gcactttttt gcgcaaacgc tgttgaacgt ttttcctgga gttctatgct agatcacatg
      241 cgcaatataa ttgaagtcac cggcctgaca aaactctttg gcaatcttgt tgcagtaaac
      301 aatctgtcgc ttgaggttct ggaaggtgaa tcatttggcc tgcttggacc aaatggcgca
      361 ggcaagacta caacgataaa aatgctcacc acactccttc tgccgacctc cgggagtgca
      421 aaaattggcg ggtatgacgt tgtaaaaaat gcctacgaag tcagaaaaat aataggctat
      481 gtgccacaac tcatatccac tgatccaatg ctgacagggc tggaaaacct gactgttttt
      541 gcgaagctct atgatgttcc tgcaagaacc agaaagtccc agattaatga ggcttttgag
      601 tttatggggc tgatggatgc caaaaacaag cttgtaaaaa actactctgg cggaatggtc
      661 agaaggctgg agattgctca atcactgctc cacaagccaa aagtgctttt ccttgatgaa
      721 ccgaccgtcg gtcttgatcc ggtggccaga aagatgatgt gggaacacat cgcaaagctc
      781 aaaaccgaat ttggcaccac gatattcctg accacccact acatggaaga ggctgataga
      841 ttgtgcgatc gtgttgccat catggaccgt ggcaagatgt cggccactgg cacaccagat
      901 agcctcaagg actcccttgg aattgagggg gcgacactgg acgacgtttt tgttcgttac
      961 actggcaatg cgttggatga aggcgctgga tttaaggaca caagacgtac tcgcagggtc
     1021 gcaaaaaggc tggggtgatt ccatgacaat tttacagacc attagcaatt tcatcaaaaa
     1081 gacctttgtc attgttgagc tggagttgaa gaagctcaag catgatccct cagagataat
     1141 aaccagggct atccaaccca ctctatggtt aatggttttt ggccaggtat tcacaaggac
     1201 tcgcgccatc cctacgggtg acataccata tattgatttc atggcccctg gaatcttgtg
     1261 ccagagcgtt ctttttatct caatcttttt tgggattaca atcatctggg agagagacct
     1321 tggaattgtg cataaatttc tcgtttctcc cgcaccaaga gaggcactcg ttcttgggaa
     1381 agctctttcg gcaggattca ggggactctc ccaagctgtt ataatcttct tgctttcgct
     1441 cctcttgggt gttggtgtta gctggagccc cctaagtatt ttgggtgtcc tcttcgccat
     1501 tgttataggg tctgcgctat tttcaacact ttcactaatc atcgcctgcc ttgtgaaaac
     1561 aagggaaaga ctcatgggta tcgggcaact cataacaatg ccgctgtttt ttgcttcaaa
     1621 tgctatttat cccatccaga tgatgcctga atggctcaaa tatattgcat ggggaaatcc
     1681 actcacatat ctggttgatg ccacaaggtc aatgatgctt gtaaagtatc agagccagtt
     1741 tggtttgacg cttgattttg ccattcttct ggcggttctt attgttttta ttttcatagg
     1801 tggcaggctc tatccgaagg tggtaaatta atcctggccg agtttctatt tatggggtta
     1861 ttgcctcata accttttgag agtaaaattc gtgagcagat gatcactggc ggtcgctatg
     1921 agctcttgaa gaaacttggc gaaggcggca tgggagaggt ttatatggct ttcgacaccc
     1981 acaccaaatc ccatgttgca ataaaatttg cttttttggg gagcagggac ccggaatccg
     2041 aaaaaaagag attttttcgt gaaattgagc tcctaaaatc gattgaccac ccaaatatag
     2101 taaaattgct ggacagtggg caggatggtg aaaacctcta ttatgtgatg gagtacatcc
     2161 agggacatga aataagggaa agggagaatt ttaaaagatt ggctggcctg ctggccattg
     2221 ttgcaaatgc cctttttgct ttgcaccaga aaggcattgt tcatggtgat atcaagcctt
     2281 caaacatcat gcttgtggaa aacaggccag ttttgctgga ttttggtgtt gcaatcgcaa
     2341 acatgcaagg acagttacga aaaggtggcg aggtctctgg gactgtaggg tatatgtcgc
     2401 cagaaggcgt aatggggcaa aaaacagatg ggtgctcaga cttgtattca ctgggagcag
     2461 ttttgtatga acagcttact ggttgtctcc cgtttggtga gggccaagtc caaaaccaga
     2521 tatttagaat attgcatgaa aaacccatca ggccatctct cgtaaatcca agggtaccag
     2581 aggaactcga agaaatctgt ctcaagctca tcaacaaaga ccctggctca aggtaccaat
     2641 ctggcaaaga agttgaagat gcgctgttgt ctttttcaag aggtgtcaca gaagaaaaat
     2701 cagccaggtc agcaataagc accgtgaagg ttcctcttgt cggaagggag gaggaagtat
     2761 cgaaatttag cgattgcctt gacctcctta tgctgggaga aggaagaatt gtcctggttc
     2821 agggtgagag cggtattggt aaaacaaagc ttgttgaaga attccggacg gtggccctgt
     2881 ccaggaaggt caaattcata ctgtgtgacg gaaaacaggc ctggcctggt gcaccagcca
     2941 ttggaagaat cttggatgaa cttgccggtt atgatatcat ggtcgacaga ccacttattt
     3001 cacggttctc gaaacagata gagttgctat ctccattatt tttcaagagg cacaacctcg
     3061 atttttcggc atacatagat gatggaagag aacaattctc ggcagcgctt gcaaaaatat
     3121 tgctttatgc gtttgatgag ggcattgttt ttgcatttga cggcataact gatccacaaa
     3181 caattgatgt gtgcaaagag atggtcattg cggctccaaa agcaaagaaa ttgcttgtgt
     3241 tctccggttt tgatccagag agtattcagc cacagaacgc ccttgccttg ccagtggttt
     3301 tggatctgaa gccacttggc agaactggaa taaaaaaact cgcggattat ctcaaattgt
     3361 cgctttctga tgatcttgca gacgaggttt tgcgtttttc aaaaggaatt ccaaaaaatg
     3421 caattgaaat tttaaggcaa cttgcgatta acccttctgc tctcaatgat ggcatgaaag
     3481 acatgaaaaa tagcgtttcg gctagaagat ttgcaggtgt tggcagaaat gccagggaga
     3541 tgcttggcgt ggtttgtctt ttggatagac cgctcccttc aagaatgatt tgtgctattg
     3601 ccagacttcc tgaggaccaa ttaggaggca ctattgatga actagtttcc aaagggtttg
     3661 ttcgcgagac tcttttggat ggggagcttg ttgttgagcc catacttccc gaagtggtgc
     3721 agtttgtaag aggtcatgtg ccggccaggg aactaacgga attgcataga cgaattgcgg
     3781 aacacctgga aaaaacattg aatgagggca gcaaagctga atatcgcaag gaggtaggat
     3841 ctcactatat agaggctggc gagtacctta gagctgcaaa atatctcatg cctgttcttc
     3901 gggaaatgca gtcaaaatat cagtttgcgc tttgtaatga catcataaac aaattactgc
     3961 cgcatttgaa caggataact gacagggaag ttgctttaag ctttagcctt agctgtgtag
     4021 cacaatttgt caatactggc ataacccctt tcttaaaaga tacctctgaa atgcttgagc
     4081 aggcgttgtt ggaagaaaac acgctggaaa acatgaagat caagtatgcc acgtggcttg
     4141 gccttgccag agcaaggctt ggcgaattca ataatgcact tgcacgtttt gagcttgcct
     4201 ggagtttttt tattgtagcg ccaaatgacg aggatggctt ttatttgacg agggctttca
     4261 gcgaggtcta tatcaactcg tcgcaatttg agaaagcaaa gaaatattgt ctccttaata
     4321 taaaatatgc taaaaagttg aaaaatgatg ccaggttatt ccagtcattt aataacctcg
     4381 gtatagtcca gatgatgatg ggaaagaatg acgaggcaga ggagatattt aaaaagaata
     4441 tcgccctttc caggaaagtt ggcagtcagc tccaagaagt aacatcattg ctgaatcttg
     4501 tcggtgttta tagcaataga aaagagtttg acaaaactat atcacttttg ctggatttga
     4561 aggacaaagc gaaagatctt ggtgatataa acaaattgtc cattatttat atcaacctta
     4621 ttaacacata cgaaaaaatc tacaggaaag aagaagctct ctcggttctt gaagaggcac
     4681 tcagtcaact gaacaagctt tcttctactt tcagactgca cacactttat gagaaagcag
     4741 tcaggatagc ccttgaaatg gagaatttcg atttggccga acacttcatc gaagatctgc
     4801 agtccagagc ttttgagtca ggaaataaat tccaggaagc ctttgcgttt gcgcttcagg
     4861 gtgacatgat gttcataaaa ggcaatcaca gggaggcgat tgtaaatctc aagattgcaa
     4921 tgaatatgtt tgaaaaagcg aagcaggagc acaaggggat atattgcatg attgcccaca
     4981 agtatgcatt ttgtgtagcg tctgagggca acataaatga agccaggaag atactaaaga
     5041 ttacaaaccc aatgctaaaa gagattttag acaaagaaat tgtaggtttt tcaatcacct
     5101 ttgaagcgtg ggttgacatg attgaatcat tctcagggaa tgtagaacaa aaaggcagcg
     5161 gcaaagaaga ctcaactttt tcaaaagatc tgtggaatag gtcatacaga aaataccgta
     5221 aagtgatgga ccctctgctt ggagtcgatg aatgtatgct acccagacct tatgccgcaa
     5281 tgggttttac gcgccttgtg cttgataggg gcaggactga tcccaaatac aaagaaatgt
     5341 tccaagagga ttgcaaaagg gccattttat atcttgatga agcaatcatg cagtgtcaga
     5401 aatcaaaaac caggctttat actggtgtcc tcataggcct tcgggagaaa acccgagatg
     5461 ccgcttctca agcttcctga tccattttgt ccagaggccg tctgatttcg ggtcgaccgg
     5521 ctcgacaaga atggttttca tcccggcgtt gtttccgccc cagatatctg tgaaaatctg
     5581 atcgcccacc attacgcact gttttgaatg gactctcatc atacgggcag cttttttgaa
     5641 tccataggaa agtggtttta gggccggatt gacaaaaggg attcccagtt tttccgatgc
     5701 ccttctgacc cgtcctggcc tgccgtttga aacaatacaa agcaggaaac ctgccctttt
     5761 gcatgcctca acccaggcaa gtgcatcttt tacgaaagca tcagaatccc tgggtgcgag
     5821 ggtgttatcg gtgtccaaaa aaacagcttt tattccccag gacatgagaa gcgaaaaggg
     5881 tatctgggac acccttttga agtatgcttt tggtttaaat gcgtccataa gtgaaagaag
     5941 agccttgcca gagtttttct acaagataaa ggaaatcttt ttcggcttcc tcggagaaca
     6001 tcactttcat gtgtcctttt ggaccaacca tgttagtttt gtcgacgatg cagatgttat
     6061 ctgcggtatc caggacactt tggacgaacc agatgtctgg ttcgtcaata atgagctcat
     6121 aagttaccat cttacaatcc tgtttgaacc gtctacctcg atggttatcc acttggcatt
     6181 ttctggtatc aggtcaataa atttgtctgg ccactcgatg acttttacac ccggtttttc
     6241 cagataatca tagtagccga ggtccacaac ttctttttcg gtttctatcc tgtaccagtc
     6301 aaaatggtag agggtgtgcc tgcccttgta ttccatgacg aggacgaatg ttggtgaaac
     6361 gatgttgtcg gtgatcccaa gacccagggc aatacccttt accagagtgg tttttccggc
     6421 gccgagcggg cccttgaggg cgtagacctc gttgccctcc atgccctcgc cccaccacct
     6481 tccaagcgct atgagctctt cctgtgaagg cactaccagt tctgtcttgt tcatggttaa
     6541 ggatagaatt tactttattt gcaaaaaaat gcaacttttc gacaaatcag ggagctttac
     6601 acggggcggc aacaagtgct aatatcaatc cataaaatgt caggaggaca aatgaaaaga
     6661 ctgctggttt ttttccttgc gctagcactt gtggcgccat cttttgttaa agctgaagaa
     6721 ccaccaattg gcatgatgcc ttatccaata gacaaagaaa tcacggatac cactgatttg
     6781 attggatatg cactctccaa acagaacgtc acaagg
//
LOCUS       DAOSRI010000068         6754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10443_length_6754_cov_4.267055, whole genome shotgun sequence.
ACCESSION   DAOSRI010000068 DAOSRI010000000
VERSION     DAOSRI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10443_length_6754_cov_4.267055"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK717_03015"
     CDS             <1..336
                     /locus_tag="PK717_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46665.1"
                     /translation="KLEISWEGFLKNLSKTNKEDSSLAKSRYIPVLLAVRLVFVERPL
                     WVIGWLGSAFFSVYGIFRLVVVGISLRGLLLVFGMFCLSILLTMTKGTLKDMIETSWI
                     IKWVDSLDE"
     gene            329..1687
                     /locus_tag="PK717_03020"
     CDS             329..1687
                     /locus_tag="PK717_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOR46666.1"
                     /translation="MNNNKINLPIVLIALGVLFAVIAIFGNVPIIFVFVALVTLGLAV
                     FFRPEIGIAALSFSAVADGLVRTYAGGVGSYWDDALYALIVVAVIWKLAKEGQLKIKS
                     GIVWWAVATFFAMSVISGLLSGLPYGHILTAIRSVLQGSIIFFVIINANFDRKVILHV
                     ASLMILCAVVVAGYGLLQRASGAFTPQGWLDKKEAVNITRATSFLGSPNATAGFLALV
                     LPMSIGMALKIKKMLPKIIYFALAAIIFFGLYATLNRAAWIGLGIGLCVFAIASNKRW
                     WIGIFMILLASAILLLPDLRLRFSTIFSDEFAVRDASLGRTFRWDTALQIFSEHPFFG
                     IGPGGFGGAVAYGIQAFGGLYVDNYYLLILSNYGLFGIASFLFLLVSSIRDAFRGLRV
                     ALEKDRYLVAGIIGGMVAFCVHLLFENLWDITPLNISFWVMAGLAASFAYKERRNENE
                     DS"
     gene            1674..2063
                     /locus_tag="PK717_03025"
     CDS             1674..2063
                     /locus_tag="PK717_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR46667.1"
                     /translation="MRIRDLMEDEVLFVYSTTSIGQMLETIIKRRINGLPVVDQDMHV
                     VGMLTEGDIISRSEMFSREFDPMQLTNMNVTTLMTKNPITIHPDAELSQAVAIFSATK
                     IKQLPVISDGKLVGILSRKDIIKGMLK"
     gene            2060..2905
                     /locus_tag="PK717_03030"
     CDS             2060..2905
                     /locus_tag="PK717_03030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOR46668.1"
                     /translation="MTIEAIILALKGYWKEKGCLIGEPFDMEVGAGTMVPYSFFGVLG
                     PRPWKVAYIQPSRRPADGRYGDSPYRFYIHHQFQVILKPPPADVQDIYLESLKVLGID
                     YEKHDMRFIEDNWESPTLGAAGVGWEVWMDGQEVTQFTYFQQAGGCELNPPSVELTYG
                     IERLAMYSERKDNGFELGWNENVTYGDLRKRAEYELCKYGFELADVESLRTMFGICEK
                     ESSRCIEAQLYMPAYEYALKCSHLFNLLDARGAVSVSERALIIARIRDLTRKCAMLYQ
                     EVGNA"
     gene            2898..4934
                     /gene="glyS"
                     /locus_tag="PK717_03035"
     CDS             2898..4934
                     /gene="glyS"
                     /locus_tag="PK717_03035"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOR46669.1"
                     /translation="MRSLLIEIGCEELPSRLVKPAIIYLMEGMCGALKEAELSFSSPK
                     TFATPRRLALLFDSVQESGPSKTVEEKGPPKSACFDKDGNPTKALQGFAVKVGIEPKD
                     VVFGEISGGVYATAKKIVPGKTIEEVVSSSLSKVVLSFPHPHSMRWDETGISWVRPIR
                     WIVAMSDDVVIDVSIGNAKSSNFTMSPRPERSRKVEIATASKYPEVLSSMGVVGNLEE
                     RSEHIFEKAQKLASSLGMEALKNKDLLDELASIVERPLVWQGEFPFDYLESAPELVIK
                     TVLMGDLRFIPFTKDGKLTNNFAFVINGPAQIAQTVLEGELKVLLGRLSDARFFFAED
                     RKKKLVDFAGKLSGVAFLKGLGTLRDKTERLVELSKMVQLGVDKNKLEKASSLAKADL
                     ASSMVHEHDDLQGRIGGLYAELDGEDGEVALAISEHYLPAGEDDPVPSSALGQALSLV
                     DKADSLVNIAASGNMPKGSADPFGVRRFAIGIIRVLVEGSLSVNLTDLLVSCSKLGFK
                     RSDDAVQKSVDFIKARLENYLRSKGYRYDVVRAAISPGIEQLKEVMGRADAMQAIYES
                     KEFAGIVATAKRLNNILRDYAPTGNYDTALFEDDVEREFEVSCKNVFKSVASSNDFAE
                     KLRLLCNLTDPAEKYFDTVMVNAEDEKIKANRKNFLSGLLQKYREVSDFVEIAI"
     gene            4943..5143
                     /locus_tag="PK717_03040"
     CDS             4943..5143
                     /locus_tag="PK717_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46670.1"
                     /translation="MKKFKVELFRKMCKSCQLCMFICPKEVFGVNERGQVVVLNDEPC
                     IGCLQCYNICPDFALEVTKNGD"
     gene            5133..6254
                     /locus_tag="PK717_03045"
     CDS             5133..6254
                     /locus_tag="PK717_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOR46671.1"
                     /translation="MAIESYLWQGSHAVAQGALKAGVNYFAGYPITPSTEVAEFMAYA
                     LPSRDGIFIQMEDEIASIASLIGASLAGAKAITATSGPGFSLMQEGLGYAIMAEVPLV
                     IVDVMRGGPSTGLPTKPTQADVMQAKWGTHGDHPIIALCPASVKESYYLMVKAVNLSE
                     RFRTPVIFLTDETIAHMRERVQIDVDEKLYVEPRVEPKDPRSYKTFQPDERLVPHQAK
                     FGTGFRTVVTALYHDESGFQNTSAEVAERFNKRLVDKVEKYIDEFYETEEYNLENSEL
                     VFVSYGASHRTVLQVLNDSKNPKWGFLRLVTIWPFHDKLVKEKLKKAKVIVVPELNMG
                     QMVREIQRLFGDTKKIVSVNRWDGELITPDQLRKVAEQA"
     gene            6251..>6754
                     /locus_tag="PK717_03050"
     CDS             6251..>6754
                     /locus_tag="PK717_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR46672.1"
                     /translation="MREQILKYFKQDKLPHVFCPGCGNGEVMHAMAQAIDELGWDPTQ
                     ILIVGGIGCSSRIATYTIFDSLHTLHGRPVAFAVGAKLANPNLKVIIVSGDGDASSIG
                     GNHLIHAARRNMDLTLFIVNNYTYGMTGGQYSPTTPLGSFTTTSPCGHVEDPFSITDL
                     LVGAGAPF"
BASE COUNT         1772 a         1454 c         1714 g         1814 t
ORIGIN      
        1 aaacttgaga tttcgtggga aggttttttg aaaaatctct ccaagacgaa caaagaagat
       61 tcatctcttg caaaatccag atacattcca gttcttctgg ctgtccgttt ggtgtttgtc
      121 gagaggccgc tctgggtaat tggttggctc ggatctgctt ttttctctgt atatggcatt
      181 ttcagacttg tcgttgtcgg tatctctctt agaggtttgt tgctggtttt tggcatgttc
      241 tgtctgtcga ttttgctcac catgacgaaa ggcaccctga aagacatgat cgaaacaagc
      301 tggatcataa aatgggtgga ctctcttgat gaataacaac aaaataaact taccaatcgt
      361 attgattgcc cttggcgtgc tgtttgctgt tattgccatc tttgggaacg tcccgatcat
      421 ttttgttttt gttgcacttg tgacccttgg gctggcggtt tttttcaggc cggagattgg
      481 tattgcggcg ctctcttttt ctgcggtggc agatggcctt gtcagaacat acgcgggtgg
      541 agttggctca tactgggacg atgccctgta cgccctgatc gttgtcgctg ttatatggaa
      601 gctggcaaaa gaaggccaac tcaagattaa atcgggcatt gtctggtggg cagtggcaac
      661 tttttttgcc atgtccgtga tttccggatt gctctccggc cttccatatg gacatatcct
      721 gactgccata aggtcagtgc tgcaaggatc gataatcttc tttgttataa tcaatgccaa
      781 ttttgataga aaggttatcc ttcatgttgc ttcgctgatg atactctgtg ctgtggtggt
      841 ggccggatat gggctcttgc agagggcatc tggagctttt accccgcagg gctggcttga
      901 caagaaagaa gcggtcaaca ttaccagggc aaccagcttc cttggaagtc caaatgcaac
      961 tgctggtttc ctggcgcttg tgcttccaat gtcgataggg atggccctca aaatcaaaaa
     1021 aatgctcccc aaaataattt attttgccct tgcggccata atcttttttg ggctctatgc
     1081 aactctcaac agagctgcct ggataggact tggcattggt ctgtgtgtct tcgccattgc
     1141 ttccaataaa agatggtgga tcggaatatt catgatcttg cttgcctcgg ccatcctgct
     1201 tttgccagat ttgagattgc gtttctcgac catcttttct gatgagtttg ccgtaagaga
     1261 cgcttccctt ggcagaacat tcagatggga cacggccctc cagattttct ccgagcaccc
     1321 gttctttggt attggcccag gcgggtttgg tggagcggtg gcctatggaa tccaggcttt
     1381 tggtggacta tatgtagata actactacct tctaatattg tcgaactatg gccttttcgg
     1441 aattgcatcg ttcctgtttc tccttgtatc atcgataagg gacgctttca gaggcctgag
     1501 ggtcgcgctt gaaaaagaca ggtatctcgt tgctggaata atcggtggaa tggtggcttt
     1561 ttgtgtccac ttgctctttg aaaacctgtg ggacatcact ccgctcaaca tatctttttg
     1621 ggtaatggcg ggtttggccg ccagctttgc atacaaagaa aggaggaatg agaatgagga
     1681 ttcgtgacct gatggaagac gaggtgcttt tcgtttacag caccacaagc attggccaga
     1741 tgctggaaac aatcataaaa agaagaatca acgggctccc ggtagtagac caggacatgc
     1801 atgtggtcgg aatgcttacc gagggagaca tcatttccag atcagagatg ttctcaagag
     1861 agtttgatcc aatgcaactt acaaacatga atgtcaccac gctcatgaca aaaaatccca
     1921 tcaccattca tccagacgca gaattgtccc aggccgttgc cattttttcg gccacaaaaa
     1981 taaagcagtt gccggtcatc tcagacggca agcttgttgg aatcctgtcc agaaaggaca
     2041 taataaaggg aatgcttaaa tgacgattga agctataatc ctggctctca agggctactg
     2101 gaaggaaaaa gggtgcctca tcggtgaacc ctttgacatg gaagtgggcg ctgggacaat
     2161 ggtgccatac agcttttttg gcgtccttgg gccaaggccg tggaaagtcg cctatatcca
     2221 gccttccaga agacctgctg atggcaggta tggtgacagc ccctacagat tctacatcca
     2281 ccaccagttc caggtaatac tgaagccacc tccagcagat gtgcaggaca tataccttga
     2341 gagcctgaaa gttcttggaa ttgattatga aaagcatgat atgcgcttca tcgaggacaa
     2401 ctgggaatct ccaacccttg gtgccgcagg tgtcggctgg gaagtctgga tggatgggca
     2461 ggaagtgacc cagttcacat acttccagca ggccggtggt tgcgagctca atccgccatc
     2521 tgtggagctg acctacggaa ttgagagact tgccatgtac tctgagcgca aagacaacgg
     2581 ttttgaactt ggctggaatg aaaatgtcac atatggtgat ctcagaaaga gggccgaata
     2641 cgaactttgc aagtatggtt ttgagctggc cgatgttgaa tccttaagga ccatgtttgg
     2701 gatatgtgag aaagagtcga gtaggtgcat cgaggcccag ctttacatgc ctgcctatga
     2761 gtatgcactc aagtgttccc acctatttaa ccttctagat gcaagagggg ccgtgtctgt
     2821 ttcggaaaga gcgcttatta ttgctcgcat cagagacttg acccgaaaat gcgcgatgtt
     2881 ataccaagag gtcgggaatg cgtagcctcc taatcgagat cggttgcgaa gaattaccct
     2941 ccaggctggt aaaaccagcc attatttatc ttatggaggg aatgtgtggg gctttgaagg
     3001 aagccgagct ttccttttcc agcccaaaga catttgccac cccaagaaga cttgcgctcc
     3061 tttttgacag tgtgcaggag tctggcccat ctaaaaccgt tgaagagaag gggccaccga
     3121 aatcggcctg ctttgacaaa gatggaaatc ctacaaaggc ccttcaaggg tttgctgtaa
     3181 aagtcgggat tgaacctaag gatgtggttt ttggtgagat cagtggcggt gtttatgcga
     3241 ctgccaaaaa aattgttccg ggaaaaacca tcgaagaagt tgtctcatct tcgctttcca
     3301 aagtggtgct gtcattccca cacccgcatt cgatgcgctg ggatgaaaca ggtatttcct
     3361 gggtaagacc gatccggtgg attgttgcaa tgtccgatga cgttgtgatt gatgtctcca
     3421 ttggcaatgc caaatcatca aattttacca tgtcgccaag gccggaacgt tcaagaaaag
     3481 tcgagatagc gactgcatca aaatatcctg aagtgctttc ctccatgggt gtggtgggca
     3541 atctggaaga acgctccgaa catatttttg agaaagcaca aaaacttgcc tcttcattgg
     3601 gaatggaagc attgaagaac aaggatcttc ttgatgagct tgccagcatt gttgaaagac
     3661 ctcttgtgtg gcagggggaa ttcccatttg attatcttga gtcagcacca gagctggtca
     3721 tcaaaaccgt tcttatggga gacctcaggt ttatcccatt tacaaaagac ggcaaactaa
     3781 caaacaattt tgcatttgta atcaatggac ctgcgcagat agcacagact gtccttgagg
     3841 gtgagttgaa agttctcctg ggaagactat ctgatgcgag gttcttcttt gctgaagaca
     3901 gaaagaaaaa gctggtcgat tttgcaggca aattgtctgg agtcgctttc cttaaaggtc
     3961 ttggcacgct tcgtgacaaa acagagaggc ttgttgaact ctcaaaaatg gtccagcttg
     4021 gtgttgataa aaacaagctg gaaaaagcat cctcgcttgc caaggccgat cttgcctctt
     4081 caatggtcca tgagcatgat gacctccagg gcaggattgg tggattgtat gccgaacttg
     4141 acggggaaga cggggaagtg gctctggcga tatccgaaca ttacctgcca gcaggcgagg
     4201 acgatcccgt accatcatct gctcttgggc aggccctttc gcttgtcgac aaggccgatt
     4261 cacttgtaaa tattgctgct tctggcaata tgccgaaggg ttcagcagat ccgtttggag
     4321 tgaggcgttt tgccatcgga ataataagag tccttgtgga aggatcgctc tctgtgaatt
     4381 tgaccgatct gcttgtttca tgcagcaagc ttggattcaa gcggagtgac gatgctgtcc
     4441 agaaatctgt tgatttcata aaggcaagac ttgagaatta cctgaggtcg aagggatacc
     4501 gttatgatgt tgtcagggcg gcgatttctc cagggattga acaattgaaa gaagtcatgg
     4561 gcagggccga tgcaatgcag gcaatatatg agtcaaaaga atttgccggc atagttgcta
     4621 ctgcaaaaag actcaacaat atcctcaggg attatgctcc aactggaaac tatgacacag
     4681 cacttttcga agatgatgtc gagagggaat ttgaagtttc ctgtaaaaac gtgttcaaat
     4741 ctgtcgcctc tagcaatgat tttgcagaaa aattgagact gctttgcaat ctcactgatc
     4801 ctgcagaaaa atactttgat acagttatgg tcaatgctga agatgagaag ataaaagcaa
     4861 acagaaagaa ctttctttca ggtttgcttc aaaaatatag agaggtctcg gattttgtcg
     4921 agatagcaat ctagggtgtc aaatgaaaaa attcaaggtt gagcttttca ggaaaatgtg
     4981 caagtcgtgt cagctttgca tgttcatttg cccaaaggaa gtttttggtg tgaatgagag
     5041 agggcaggtt gttgtgttaa acgacgagcc gtgcatcggg tgtttgcagt gttataatat
     5101 ttgccccgat ttcgcactgg aggtgaccaa aaatggcgat tgaatcatat ctttggcaag
     5161 ggtctcatgc tgtagcccag ggcgccctga aggccggggt caattatttt gccggatacc
     5221 caataactcc ttcaactgaa gtagctgaat tcatggctta tgccctgcca tcaagagatg
     5281 gaatttttat tcagatggaa gacgagattg catcgattgc atcgctcata ggtgcttcac
     5341 ttgctggtgc gaaagccata acggcaacat cagggccagg attctcttta atgcaggaag
     5401 gccttgggta tgccataatg gcggaagttc cacttgtaat tgttgatgta atgaggggtg
     5461 gtccatcaac aggccttccc actaagccaa cccaagccga tgtaatgcag gcaaaatggg
     5521 gcacacatgg tgaccatcca ataattgcgc tctgtccagc atcggtcaag gaatcttatt
     5581 acctcatggt gaaagcagtc aatctctctg aaaggttcag aacgccagtc attttcctga
     5641 ccgatgaaac cattgcccat atgagggaga gggtccagat tgatgttgat gaaaagctct
     5701 atgttgaacc acgcgtagag ccaaaagatc caagaagcta caaaacattc cagcctgacg
     5761 aaagattggt gcctcaccag gctaaatttg gaactggttt caggacggtt gtcacagcac
     5821 tttatcatga tgagtccggt ttccagaata cctctgccga agttgcagaa aggttcaaca
     5881 agaggctggt ggacaaggtc gaaaaatata ttgacgagtt ttatgaaaca gaagagtata
     5941 acctcgaaaa ctcggagctt gtctttgttt cctacggtgc ctctcacagg accgttcttc
     6001 aggtgctaaa cgattcaaag aacccaaaat ggggcttttt aaggcttgtt acaatatggc
     6061 ctttccatga caagctcgtc aaggaaaaac tgaaaaaagc caaagttatt gttgttccag
     6121 agctgaacat gggtcagatg gtcagagaga tccagaggct ttttggcgac acaaagaaaa
     6181 ttgtttccgt caacagatgg gacggcgaac tgataacgcc agaccagctt agaaaggtgg
     6241 ctgagcaggc atgagggaac aaatcctgaa atatttcaaa caggacaagc tgccacatgt
     6301 tttttgtcct ggctgtggaa atggtgaagt aatgcatgca atggcccagg ccatagacga
     6361 gcttggctgg gacccaacac agatactgat tgtaggtgga atagggtgtt catcaagaat
     6421 tgccacatat acaatctttg attctttgca caccttgcat ggaaggccag tggcttttgc
     6481 tgttggcgca aagcttgcta acccgaacct caaagtgatc attgtgagtg gtgatggtga
     6541 tgcctcttca attggcggca atcaccttat ccatgctgca aggcgcaaca tggacctgac
     6601 cctctttatc gtcaataact acacatacgg aatgactggc ggacaataca gcccaacgac
     6661 accacttggc tcgttcacga ccacttcgcc atgcgggcac gtggaagacc cctttagtat
     6721 cacagacctt ctcgttggag ctggtgcacc gttc
//
LOCUS       DAOSRI010000069         6733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10488_length_6733_cov_3.814915, whole genome shotgun sequence.
ACCESSION   DAOSRI010000069 DAOSRI010000000
VERSION     DAOSRI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10488_length_6733_cov_3.814915"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3298)
                     /locus_tag="PK717_03055"
     CDS             complement(<1..3298)
                     /locus_tag="PK717_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46673.1"
                     /translation="MKKTISTIIISILVMSLPVSRPTSISAKEEISIKPTSSAIELSW
                     GAVSGASFYQITRVSPKKDLFPKPVAQNSWVDESPSTDTPNTYEVRALSNSGQVVSTL
                     PEVTASVDLNTPTLSKPCHVVLSFAIGSKSYTENGTIKTMAVAPLSRDGRTYIVIKHV
                     VEPLFGTIGWEAKTKKVTIEALGKKIEMWVGKPVAKIDGKNTPIDPKNSKVAPFIEGG
                     RTYVPLRFPVESLGRGKIDWYQKTKTAVLAFPIGSGKWAEGTVKNGSVSFDQTTLKVE
                     GIKAGSCVGIQYESLPGSKVVVGRYARVPSSTNRCEGNEISGTVDKVDGSNVAISGQS
                     FRLSSGLSTPSAGSCIRACVVQGSIVSYKLSVCPGKGYEGQIQLVRCQDGLISVNALG
                     TTITVRLPQGFDCASLSAGQCIRFDGRPDPSDQTQINATVVEIVQCRVGQEMVLFTQG
                     TCSESQIEVQDVSGNSFTLKVPEDVPCGDIQPGECLKVFAKKEGNVLVASEADIVQPP
                     KDEKFYRLAFVTSVSPLRVTVPGGNTFTLVLPKYFKTQLKISDGLDIWGAVSGSTIKS
                     PRVVSAPNAGKYLKGEVVGVVCEKKSVLVNEISSKTTVVLSDLANCESIQFGECYSFF
                     GVQTEKEFLACTLEKTECPKGCQGETYEGTIVEKDCQAGTSKIRTSTGDYLSVKLPQG
                     FDCSSLKKGLCLEICGNLEGGVITAQKISFKDCREPVCTGKLVEGLIVSTNCKDGTFR
                     FATKDGELQLQMPETQECEDVPVGSCAQVCIVGESKTYYIKTIPCSKFGSVLEGTIIQ
                     SVKDSYTVQTKEGNEIQLLWKEGLSLGDCIIALGNYLPDSTTQFRADIVAKTTCGGVE
                     NISGSVTLVDCAKKTIEVATESGTQEINLPEDADCKTFLPGDCVFVRHKDGKTLINKV
                     DCPQTKRAHFAMFVIGNNKETVYGTDLATLDRFECVGQKPPAAGSVVIVQGHQVSSRK
                     VLQATFTPYDIGCVQTKPVSGKLISYREETRAGTIEDVFGNSYVMIFPGDRTFAVGDY
                     LSLKVQVYDTGLGTRCMIATELYKSSEGGIAQANVTGVIFAIDPVEGAALLHVSSGEN
                     IAVMPSDPETL"
     gene            complement(3312..3839)
                     /locus_tag="PK717_03060"
     CDS             complement(3312..3839)
                     /locus_tag="PK717_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOR46674.1"
                     /translation="MKEHFGEEAEVVNRIRADYLRRYGSTLAGLMIHEKIDPRKFVDF
                     VHDVDLTLYLSPNPVLRDVLESLACPIYIASNAPRFHVESILKVLGVDHIPLKIFSIE
                     SFGYEGKPNHSSYRHLLEQTSTMPGEAMLVDDYTLNLRGAREVGFATCLIGNEEWEPR
                     LERLELISSLPGITH"
     gene            3966..4790
                     /gene="amrB"
                     /locus_tag="PK717_03065"
     CDS             3966..4790
                     /gene="amrB"
                     /locus_tag="PK717_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOR46675.1"
                     /translation="MIRLSCNMVMDRKPYVAGRFYPDDPQKLGSMLESFFEGADTKTA
                     VPSIIVPHAGYMYSGKVAGAIYSKIVVPEIVVIIGPNHTGLGAPISVWPDGSWETPLG
                     NVPVDKGTTIALLNNSKHAKPDTQAHLNEHSIEVQIPFLQYCKKDFKIVPIAMGDYSI
                     EAIEDLANALDKATSGKEVLFVASSDFSHYEPEYTAKARDRMAIDEIKKLDWMAMLRV
                     VAEKEITMCGAGPIAVATELAKRRGATSGKLLAYSNSGVMSHDFSNVVTYAGMAFR"
     gene            4787..6283
                     /locus_tag="PK717_03070"
     CDS             4787..6283
                     /locus_tag="PK717_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46676.1"
                     /translation="MRPGKTAIAVLASLLLCLPQALTTAKTDSKISVIFGQAFSVGAR
                     VFVATDTIYSKTTLPFSPEIMDCPGLEITGKPWLVTNGRTVLTLGRFDATYSCFEPTQ
                     LVGLDLEKVSGKIEESSINSSGRLIKADFSRSIFLKNGEPSKMADLRDFDVECFGEYS
                     GEIFRVFLALDTDNTIQSLSGIIKEIGDEEVVFEATKNWGKQKELTRIVAKPSEWCVF
                     LRGNAPVENMKGLSKGKLSALLLRKNLAGEDEAIAIQSDLLATTSGGRILGLATGTGD
                     GSIKIKTVFQGGIPATLNAKIGTLTKILENGNSITQEKLFELVAARPLPAEVIGNFEQ
                     DQFSVKASLVRINPLGPSEYFCGIAKGDVATNFFGKTRKLVFSDQAKFLYGTPPLTDG
                     NPVECWFEGDKIVAFGLPQIGILGFPLEGRFLGSEGDTILLDCYGSYDRSLAGQKIKL
                     YLNRATKFIRGGEGEVPPSEIPFGQRLSCFGVFNNGKFVVMMASLIDG"
     gene            6332..>6733
                     /locus_tag="PK717_03075"
     CDS             6332..>6733
                     /locus_tag="PK717_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46677.1"
                     /translation="MKFRVFVIVLLLVCIAIPIPTSIATFDLNGRPLWPIVPTSEANI
                     LVLEYHSISKAPQNAPFPDLYVSMDSFKKQMDILMELGMTGTSFIDCIYQMKAGHVNL
                     ANVVLTFDDGYADNYWVGQYLSSLKFSATFFI"
BASE COUNT         1761 a         1639 c         1554 g         1779 t
ORIGIN      
        1 tcaaagtctc tggatcagaa ggcatgactg cgatattctc tccgcttgaa acatgaagga
       61 gcgcagcacc ttcaaccggg tctattgcaa atatgacgcc tgttacgttg gcctgcgcaa
      121 tgccaccttc agatgatttg taaagttctg tggctatcat gcaccttgtg ccaaggccgg
      181 tgtcatagac ctggactttc agcgaaaggt agtcgccaac agcaaaggtc ctgtcgccag
      241 ggaaaatcat cacatagctg ttgccaaaga catcctcgat cgttcctgcc cttgtttctt
      301 ccctgtagct tatgagcttc cctgaaactg gttttgtctg gacacagcct atgtcatatg
      361 gggtaaaggt ggcctggaga acttttctgg atgacacctg atgtccctgg acaatcacga
      421 ccgaaccagc ggcaggcggc ttctgcccaa cacactcaaa ccgatcaagt gtggcaagat
      481 cagtcccata gactgtttct ttgttgttac caataacaaa cattgcaaaa tgggcccttt
      541 tggtctgcgg gcaatcaact ttgttgatga gggtcttgcc gtccttgtgc ctcacaaaaa
      601 cacaatctcc aggcaaaaat gtcttgcagt cggcatcttc tggcaggttt atctcctggg
      661 tgccagattc tgttgcaacc tcgatggttt ttttggcgca atcaacaaga gttactgacc
      721 cgctgatgtt ttcaacacca ccgcaggtgg tctttgcaac gatgtctgcc ctgaattgtg
      781 tcgtgctgtc agggaggtaa ttgccaagcg caataatgca gtctccaagg gaaagacctt
      841 ccttccagag gagctggatt tcatttccct ccttggtctg gaccgtgtat gagtccttta
      901 ctgactgtat gattgtccct tcaaggacag acccgaattt ggagcagggt atggttttta
      961 tgtaatacgt tttcgattca ccaacaatgc aaacctgcgc acatgagcca acaggaacgt
     1021 cctcgcactc ctgggtctcc ggcatctgga gctgtagctc tccatccttg gtagcaaacc
     1081 tgaatgtgcc gtccttgcag ttagtggaaa caatgagccc ctcgacaagc ttgccggtgc
     1141 aaaccggttc cctgcaatcc ttaaaagata ttttctgggc agtgataacg ccgccctcaa
     1201 ggtttccaca aatctcgagg caaagacctt ttttgagtga tgaacagtca aatccctgtg
     1261 gcagcttgac tgacaggtaa tccccggtag aagtccttat tttcgaagtg ccagcctgac
     1321 agtctttttc gacaatggtg ccctcgtaag tttctccctg gcaacctttt ggacactcgg
     1381 ttttttcaag tgtgcaggca aggaactctt tttctgtctg taccccaaag aaagaatagc
     1441 actcgccgaa ctggatgctc tcacaatttg cgaggtccga aagcacaacc gtggttttgc
     1501 ttgaaatctc gtttacaagc acagacttct tttcgcaaac aacgccaaca acctctccct
     1561 tgaggtattt tccggcattt ggcgcagaaa caaccctcgg gctttttatt gtcgaacctg
     1621 aaactgcccc ccaaatatca agcccgtcag aaatcttcag ctgggttttg aagtattttg
     1681 gaagcacaag cgtaaatgtg tttccaccag gaacagtaac cctgagcggg ctcactgaag
     1741 tgacaaaagc aagcctgtag aacttttcgt ccttcggtgg ctggacaatg tcggcctctg
     1801 aagccacaag cacattacct tctttttttg caaaaacctt gaggcactca cctggctgga
     1861 tgtctccgca gggaacatcc tccggcactt tgagcgtaaa gctgttgcca gagacgtcct
     1921 gaacttcgat ctggctttca gagcaggtgc cttgggtgaa gaggaccatt tcctggccaa
     1981 ccctacattg gacgatttca acgactgtgg cgtttatctg cgtctgatct gatggatcag
     2041 gccttccatc gaacctgatg cactggccgg cggaaagcga agcgcaatca aaaccctgcg
     2101 gcaatctgac cgttatggta gtgccgaggg catttaccga aataaggcca tcctggcacc
     2161 tgaccaactg tatctggcct tcatagccct tgccagggca aacagaaagc ttgtaggaaa
     2221 caatcgaacc ttgcacaaca caggccctga tgcaggaacc ggcagagggt gtggaaagac
     2281 ccgatgataa acggaacgat tggccagaaa tcgccacatt tgaaccgtca accttgtcga
     2341 ctgttccaga gatttcatta ccttcacaac gatttgttga cgatgggacc ctggcgtacc
     2401 ttccaacaac cacctttgaa cccggcaggc tttcatactg gataccaaca caggaaccgg
     2461 cttttatgcc ttcaaccttg agggttgtct gatcaaatga gaccgagcca ttttttacgg
     2521 tgccttctgc ccatttgccg gagcctatag gaaaggccag gacggcggtt ttagtcttct
     2581 ggtaccagtc tatcttacct cttccgagcg actcgaccgg aaagcggagc gggacgtaag
     2641 tccttccgcc ctcgatgaac ggggcgacct tggaattttt ggggtcaatt ggagtgttct
     2701 tgccatctat ttttgcaacc ggtttcccga cccacatttc gatttttttg ccaagggctt
     2761 ctatcgttac tttctttgtt ttggcttccc agccaattgt tccaaaaagc ggctctacaa
     2821 catgcttgat tacaatatag gtcctgccat ctctggaaag tggcgctaca gccattgttt
     2881 ttatggtgcc attctcggtg taggacttgc tgccgatggc aaagctgagc acaacgtggc
     2941 acggcttgga aagtgttggc gtattgagat caacagatgc agtcacctca ggaagtgtgg
     3001 agacaacctg cccggagttt gaaagggctc ttacctcgta agtatttggc gtgtcagttg
     3061 aaggcgattc gtccacccag ctgttctggg caacaggttt tggaaaaagg tccttctttg
     3121 gggaaaccct cgtgatttgg tagaatgatg ctcctgaaac agcgccccag gaaagctcta
     3181 ttgcactgct ggttggtttt atcgatattt cttcttttgc tgaaatactg gttggtcttg
     3241 aaacagggag ggacatgaca agtatcgata taatgatggt ggatattgtt tttttcatat
     3301 ttgttcccct tttaatgcgt tataccaggc agactggaaa tcagttcaag cctctcaagc
     3361 ctgggttccc actcctcatt gccaataaga caggtagcaa aacccacttc tctggcacct
     3421 ctgagattta atgtgtaatc atctaccagc attgcttcgc ctggcattgt tgatgtctgc
     3481 tcaagaagat gcctgtaact gctatgattc ggttttcctt catatccaaa gctctctatg
     3541 gagaatattt taagtggaat gtggtcaaca cccaacacct taagaatgct ctccacatgg
     3601 aatcttggcg catttgaggc aatgtatatt ggacacgcca gggattccaa gacatctctt
     3661 aacacggggt ttggggaaag atacaatgtg aggtcaacat cgtgaacaaa atcgacaaac
     3721 tttctaggat caattttttc atggatcatc aatcctgcaa gcgtagaccc atatcttctc
     3781 agataatcgg cccttatcct gtttacgacc tcggcctctt ccccaaaatg ctccttcatg
     3841 aaaagcacca tgcgtgacct aatgttctct tccaggttgt tattcctata gagagtctgg
     3901 tcacaatcta gaaacaactt cataatgcaa tgctaaacaa atttcccagc aattgacagt
     3961 aacaaatgat aagattatct tgtaatatgg ttatggacag aaaaccttat gtcgcaggaa
     4021 gattttaccc tgatgatcca caaaaactcg gatcaatgct tgaatcattt tttgagggtg
     4081 cagacacaaa aacggcagtg ccatcaataa tcgtcccgca cgctggatat atgtattcgg
     4141 gaaaggtggc aggcgcaatt tattcaaaaa tagttgttcc tgaaatcgtt gtcatcattg
     4201 gcccaaatca tactggactc ggagcaccca tttcggtttg gcctgatggg agctgggaaa
     4261 caccacttgg caatgtgcca gttgataaag gaacgactat agccctgcta aacaattcca
     4321 aacatgcaaa accagacacc caggcccatc tcaatgagca cagcattgaa gtccagatcc
     4381 cattcctcca gtactgcaaa aaagatttca agatagtgcc aattgccatg ggtgactaca
     4441 gcattgaggc gattgaagac ctggcaaacg cacttgacaa ggcaacctct ggaaaagaag
     4501 tgctctttgt cgcttcctcc gatttctccc attacgaacc agaatacacc gccaaggcca
     4561 gggacagaat ggccatcgac gagataaaaa agcttgactg gatggcaatg ctccgcgtgg
     4621 tcgccgaaaa agaaataaca atgtgcggcg ctggcccgat agctgtagca accgagctgg
     4681 caaaacgcag gggtgcaacg tccggcaaac tgctggcgta cagcaacagc ggggtcatga
     4741 gccacgattt ttccaatgtc gtgacatatg ccggcatggc gttccgatga ggcccggcaa
     4801 aacagcaata gctgttctgg catcattgtt gctgtgtctt cctcaagcac taaccacagc
     4861 aaaaaccgat tccaaaatat cagttatttt tggccaggca ttttcggttg gcgcaagagt
     4921 ttttgtcgcc acagatacaa tctattcaaa aaccaccctc cctttttcgc ctgaaatcat
     4981 ggactgtccg ggactggaaa taacaggcaa accctggcta gtgacaaacg ggagaaccgt
     5041 tcttacactt ggcagatttg atgccaccta ttcatgcttc gaaccaacac agcttgttgg
     5101 tttagacctg gaaaaagttt ccggaaaaat tgaagagagc agcataaact cttcgggtcg
     5161 cctcataaag gctgattttt caagaagcat ctttctcaaa aatggtgaac catcgaaaat
     5221 ggctgacctg agagatttcg atgtggaatg ttttggtgaa tactctggcg aaatattcag
     5281 agtttttttg gcattggata cagacaacac gattcagagt ctttctggca tcataaaaga
     5341 gattggcgat gaagaggttg tttttgaggc cacaaaaaac tggggcaaac aaaaagagct
     5401 gaccaggatt gtggcaaaac catctgaatg gtgtgtcttc ctgaggggga atgcacctgt
     5461 cgaaaacatg aagggattat caaaaggaaa actctccgca ctccttttga gaaaaaatct
     5521 tgctggtgag gatgaagcaa tagcaatcca gtccgatctc ctcgcaacca catcaggtgg
     5581 aaggattttg gggcttgcca ctggcaccgg tgatggctcc atcaagataa aaactgtctt
     5641 ccagggtggc attccagcaa cgctaaatgc aaaaatcggc acactgacaa aaatccttga
     5701 gaatggcaat tccattacgc aggaaaaact ctttgagctc gtggccgcaa gaccacttcc
     5761 agctgaagtg attggcaatt tcgagcagga ccagttttcg gtgaaggctt cgcttgtgcg
     5821 aatcaacccg cttgggccca gtgagtattt ttgtggcatt gcaaagggag atgttgccac
     5881 caattttttt ggcaaaacca gaaaactcgt attttccgat caagccaaat tcctctacgg
     5941 cacgccaccg ctaacagatg gcaaccccgt cgagtgctgg tttgaaggcg acaaaattgt
     6001 tgccttcggt cttccccaaa ttggcattct tggatttccg cttgaaggca ggttccttgg
     6061 ctctgaagga gataccattc tcctggattg ctacggcagc tatgaccgga gtctcgcagg
     6121 gcaaaaaata aaactctacc tgaatagagc aacgaaattc ataaggggtg gcgagggtga
     6181 agttccccca tccgaaattc cttttgggca aaggctgtca tgttttgggg tttttaacaa
     6241 cggaaaattt gtagtgatga tggccagcct tatcgacggc taaataatcc ctcggcttgt
     6301 aaagaatgta tcaatgctgt aaagttttct catgaaattc agggtttttg taattgtttt
     6361 gcttcttgtg tgtatcgcta tcccgatccc gacctcaatt gccacatttg atctcaacgg
     6421 gcgcccgcta tggccaatcg ttcccacctc tgaagccaat atcctggtcc tggagtacca
     6481 ttcgatatcc aaggcgccac aaaacgcacc attccccgac ctctacgttt caatggattc
     6541 attcaaaaaa cagatggaca tactcatgga gctcggaatg accggtactt cttttattga
     6601 ctgcatatac cagatgaaag ccgggcatgt caatcttgca aacgtagttt tgacctttga
     6661 tgatggttac gcagataatt actgggtggg ccagtattta tcgagtctca agttttcggc
     6721 aaccttcttt att
//
LOCUS       DAOSRI010000070         6722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10507_length_6722_cov_3.917504, whole genome shotgun sequence.
ACCESSION   DAOSRI010000070 DAOSRI010000000
VERSION     DAOSRI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10507_length_6722_cov_3.917504"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..2240)
                     /locus_tag="PK717_03080"
     CDS             complement(108..2240)
                     /locus_tag="PK717_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46678.1"
                     /translation="MKSRLITAMLAIILALQMPVFAMAEEGKCDDWVMFNHDPSRSCL
                     TTNQAAPPLLVGEAYKATAAIHSSIAMTDRYSVVGSDDMRIYCYDTHSFRIRWNYLTG
                     NKVRSSPTIFGEVTICGSDDGYLYCLNLETGNEVWKFKTGDIIRSSPLVVNGKVYFAS
                     RDMKVYCLDVLSGQKVWAFHTGAFVDASPTYNEHNKTILIGSYSKKLYCLNAENGRLT
                     WEFETPSEVFAACSVRNEKIFFGTDGGQFYCLSDDGRQIWKIDVGAHVWATPALVEDK
                     LIIPTGYDKMVRCLYQDTGEEVWNFRTENWNYSSPAVGGKYVFFGSDDQNLYCLYWKT
                     GQLAWKGNLGYIIEASPVIYCEAVYVGTWGGTVQVFQPGPILEVEPQEIDFGIVTHGT
                     TPFRDIIIRNARKDQFQTKLEGKMVTDEKHLSFSATEFFDISNGSEQKIRVTLDPGGL
                     DYGNHQALLKISSNGGEFTMIIRWRIVSPAPPCLNVSPSELNFGFIKRGEEVSKTLTL
                     SFDTDEEVSGIVMGEDRWIDVEPVSFTSINRKFLLKITLNGSRIPAGNEAQGRIVIAT
                     KNDVCQQVSVMVKVETEPRIEISMVIGDNTAFVNGRPVDMDVAPYVTSKGRTMVPIRF
                     ISETFGADVKWIADKKEIQINRFDNRVKLWVGRPEMDVNDLIKAIPSPPEIKDGRTMV
                     PFRAIAEAFGADVVWIAETKTVRMVFNP"
     gene            complement(2444..6283)
                     /locus_tag="PK717_03085"
     CDS             complement(2444..6283)
                     /locus_tag="PK717_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46679.1"
                     /translation="MKKITARALALLIVMSSVFVSGPGVAASCDCTRFKFDLGNNGVF
                     PSECAPENNDLVKRWSYMTGGSVTCAPLVVSGKVYVASWDNKMYCLDAEKGTLSWSAK
                     IGGVLNGICYDSDRVYASCTDKRVYCLDAKTGKQLWVFTMSSASISNPLVYLGKLYAA
                     CGKTNDKTNKGDNYIYCLDAKTGKAIWKVKNQVGFEASPAIAGGKIVIGSNDNCMYCF
                     DATNGKQLWKSARVGSEFDAPASISVASGKIYAGNWDKKLYCLDLKTGKTLWTYLAGG
                     PILTTPTIAEGKIYFGSDDGNLYCVDGSSKKLSWKYKTDGKINNSSPVYCAGKIYFGS
                     YDNKLYCLDAKTGKELSSFETKDEVWSTPVICNKKIYFGSNDSNVYCLEARVEKPVKM
                     TIKPKFAVLKPGQSILFTATVYNNYDEVMKDQKITWSLTDPKTGTLDYGGLFGAKSPG
                     LTDIVATSGQLSARAKVYVYDEGLNLEKQGCDWPCFGGDPSRTSSTEADCGPKTDKLK
                     ILWKYNAKSPLTTQAISVDGMVFAGAQDGKFHCMDLETGAKIWEFKSGALAYTPVYWQ
                     GKLFAPSFDKKLYCLDAKTGKELWSFEAGGEIRTSPTIYQGKLYFGCGKVRDDNENNS
                     GKNALYCLDVLTGKLVWENPNKIDFESSPTVADGKVFIGSSDDTYYCFDANTGDILWT
                     IDSKNDFDGETTYFDGRIYFSTWDGIVRCVDPRSGDAIWTSEIDKAVFGPLAIRDGRI
                     FAISTNKRMFCLSLVDGKVLYDVPMEGSSFYSVACYGNRVVLGDTGGLIKLLDASTGE
                     EKFAYNVKGGKDFVYPSVCGGKILFTNTDGFLYCFVDDDTVEPEKPYPARLVVQPKES
                     VLKPGETQQFTATVFDQFDQEMKDIQISWECSDAEIGSVDQNGLFTAIKDGECTVTAT
                     YLELSDTGKVKVLKIAKPEEVTCEWSQYRGNLQRNGVVPDGCGPDPAKTELIWSFETG
                     DDIYSSPTISDGRVFVGSDDNHLYCLDLASGSKIWDFTATGRVFSPAVDSGRVYACSY
                     DRNLYCLDAKTGNKIWSFMSQGPTFSSPVVWENRVYFGSKDHFMYCLDATTGKLLWKF
                     EAPDQVDSSPALWEGKIYFGCEDKSLYCLDALTGKKIWDFKTGYMITSTPAIWNGKVY
                     IGSDDKKLYCLDAQTGSQVWMFEAGGVLHYSSPAIWNGRVFVGATDSFLYCLDSETGK
                     KLWTFRAEGWVTSPAVCGDKVWFGSQDYYFYCVDFNTGEKIWSFKTEGAVSSPAISNG
                     HILAGSFDNKLYCFGAK"
     gene            complement(6331..6519)
                     /locus_tag="PK717_03090"
     CDS             complement(6331..6519)
                     /locus_tag="PK717_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46680.1"
                     /translation="MEKLEIAHKRLFDAAYYAAEAIKKAIDLNVLPKNLAEEMSQALD
                     DLDVAMESSKKHFVDPGC"
BASE COUNT         1712 a         1693 c         1471 g         1846 t
ORIGIN      
        1 ctgctcctgg atgcctgatt gagagagcca ggcggacgca gaaagaaggg cctttattgc
       61 atcaccattt gcgcgcccgc ttgtccacga gagcgcattg gcaagggtca tgggttgaag
      121 accatcctta ctgtttttgt ctcggcaatc cacactacat cggcgccaaa ggcctcggct
      181 attgccctga acggaaccat tgttctgccg tcttttatct ctggcgggga ggggatggct
      241 tttatgagat cattgacgtc catttcagga cgacctaccc agagcttgac cctattgtca
      301 aacctgttta tctgtatctc tttcttgtcg gcaatccatt tgacgtcggc accaaaggtc
      361 tcggagataa accttatcgg aaccattgtt ctacctttgc ttgttacata cggggcaacg
      421 tccatgtcaa caggcctgcc atttacaaaa gccgtgttgt cgccaatgac cattgagatc
      481 tctatcctgg gctcggtttc gacctttacc atgacggaaa cctgctggca aacatcattt
      541 ttcgtggcaa tgacaatcct tccctgggcc tcgttgcctg ccggtattct actgccattg
      601 agtgtgatct ttagaaggaa cttgcgattt atcgaggtga aagagactgg ttctacatca
      661 atccacctgt cttcacccat cactatgccg gagacttcct catcggtatc aaaagaaagg
      721 gtcagcgtct ttgagacttc ctcacccctt ttgatgaacc caaaattgag ttcggatgga
      781 gaaacattca ggcatggtgg tgctggtgaa acaatgcgcc acctgattat catggtgaac
      841 tcgccgccat ttgaagatat cttgagcaac gcctggtggt tgccataatc aagcccaccg
      901 ggatcaagcg ttaccctgat tttttgctca gatccattgc ttatatcaaa aaattcggtc
      961 gcactgaaag aaagatgttt ctcatcagta accatttttc cttcgagttt cgtctggaac
     1021 tggtcttttc tggcatttct gatgatgatg tccctgaaag gtgttgttcc atgggtcaca
     1081 ataccaaaat caatttcttg cggctcaact tcaagaattg gccctggctg gaagacctgg
     1141 actgttccac cccatgtgcc gacataaact gcctcgcagt agattacagg cgacgcctca
     1201 ataatgtatc caagattgcc cttccaggcg agttggcccg ttttccagta cagacagtag
     1261 agattctggt catccgaccc aaagaaaacg tattttccgc cgacagccgg ggaggagtag
     1321 ttccagtttt ccgtcctgaa attccagact tcctcgccgg tatcctggta gaggcaacgg
     1381 accatcttgt cgtatccagt tggaattatg agtttgtctt caacaagtgc tggcgttgcc
     1441 cagacgtggg caccaacatc tatcttccag atctgcctgc catcatcgga cagacaatag
     1501 aactgaccgc catctgtgcc aaaaaatatc ttttcgtttc tgacagaaca ggccgcaaaa
     1561 acctcagaag gggtctcaaa ttcccaggtc agcctgccat tttcggcatt gaggcaataa
     1621 agcttctttg aatatgaacc gatgaggata gtcttattgt gctcattgta ggtcggcgag
     1681 gcatcaacaa aagcacccgt atggaatgcc cagactttct ggccagaaag gacgtcaagg
     1741 caataaacct tcatgtccct tgatgcaaaa tagaccttcc cgttaacaac aaggggtgac
     1801 gatcttatga tatcgccagt tttgaatttc cagacttcat ttccagtttc aaggttcagg
     1861 caatagaggt agccgtcatc cgagccacag attgttactt ctccaaaaat tgttggtgac
     1921 gagcggacct tgtttccagt cagatagttc caccttatcc tgaatgagtg agtgtcgtag
     1981 caatatatcc tcatgtcatc agaaccaacc acgctgtacc tgtcggtcat tgcaattgag
     2041 ctgtggatgg cggccgtggc tttgtaggcc tctccaacaa gaagcggcgg tgccgcctgg
     2101 ttcgttgtca gacaagacct cgaaggatca tggttaaaca ttacccaatc atcgcacttt
     2161 ccttcttcgg ccatggcaaa aacaggcatc tggagagcta gtataatagc caacattgct
     2221 gtaatcagtc tggatttcat gagagagata ttagcacaat aaattaaatc aatcaattga
     2281 acgcagactt ggacaaggtc aatttattga aaaatctaca ttaaaattac agtgttttct
     2341 ccaatcaaaa taaagaaatc ccatatttat gcacaaattg ttataaacaa aaatacgccg
     2401 aaggtttccc cctcgacgta tctgaagctt tgtgtgttcc cggttatttt gcaccaaaac
     2461 agtagagttt gttgtcaaaa gagcctgcca gtatatggcc gtttgaaatg gcaggtgatg
     2521 aaacggctcc ttctgttttg aaagaccata tcttctcgcc agtgttgaaa tcaacgcagt
     2581 agaaataata atcctggctg ccaaaccaga ccttgtcacc acaaactgcc gggctggtta
     2641 cccacccttc cgccctgaac gtccaaagct ttttgccagt ttcagaatcc aggcaataaa
     2701 ggaaggaatc agtcgcaccc acaaacaccc tcccgttcca gatcgcaggt gaagagtagt
     2761 ggaggacacc gcctgcctca aacatccaca cctgggagcc agtctgggcg tccaggcaat
     2821 aaagcttctt gtcatcggaa ccaatgtaga cctttccatt ccagatggca ggcgttgagg
     2881 ttatcatata gccagtctta aagtcccata ttttcttgcc agttagcgcg tccaggcaat
     2941 aaaggctctt atcctcacat ccaaaatata tcttgccttc ccagagcgca ggcgatgaat
     3001 caacctggtc gggtgcctca aacttccaga gcagtttccc tgtggtggca tcaaggcagt
     3061 acataaaatg gtcctttgac ccaaaatata ccctgttttc ccacaccact ggggaggaga
     3121 atgttgggcc ctgggacata aatgaccata tcttgttacc tgtttttgcg tccaggcagt
     3181 agaggtttct gtcataggaa caggcataga cccttccgga atcgactgcc ggagagaaca
     3241 ccctgcctgt agcggtgaag tcccatatct ttgacccact cgccagatcg aggcaataga
     3301 gatgattgtc gtccgaaccg acaaaaaccc tgccatcgga gatggttggc gaagaataga
     3361 tgtcatcacc tgtttcaaag ctccagataa gttccgtctt tgccgggtca ggcccgcaac
     3421 catcaggaac aacgccattt ctctggaggt tgcccctgta ctggctccac tcgcaagtca
     3481 cttcttccgg ttttgcgatc ttgagcactt tcacttttcc ggtgtcagaa agttcaaggt
     3541 aagtagcggt tacggtgcat tccccatcct tgatggcagt aaagaggccg ttctggtcaa
     3601 ctgacccgat ttcagcatca gagcattccc agcttatctg gatatctttc atctcctggt
     3661 cgaactggtc aaaaaccgtg gccgtaaact gctgggtctc gcctggtttc aggactgatt
     3721 ccttcggctg gacaacaagt ctggccggat atggtttttc aggctcgaca gtgtcatcat
     3781 caacaaagca gtagaggaag ccatcggtgt ttgtgaagag tatctttcct ccacaaactg
     3841 atggatacac aaaatccttc ccgcctttta cattgtaggc aaatttttcc tcgcctgtag
     3901 aagcgtcaag caacttgatc aatccaccgg tatcgccaag aaccacccta ttcccgtagc
     3961 aggccacgga ataaaacgat gagccttcca ttggcacatc ataaagcacc ttgccatcca
     4021 caagcgagag gcagaacatc ctcttgtttg tggaaatggc gaaaatcctg ccgtccctta
     4081 tcgccagagg gccaaaaact gccttgtcta tttcactcgt ccagatggcg tcaccggaac
     4141 ggggatcaac acaccttaca atgccgtccc atgtggagaa atagatcctg ccatcaaaat
     4201 atgttgtttc accgtcaaaa tcgttcttgc tgtcgattgt ccacaaaata tcgcctgtgt
     4261 ttgcatcaaa gcagtagtag gtgtcatcac tggacccgat aaatactttg ccatcggcaa
     4321 cagtcggtga tgactcgaaa tctatcttgt tcgggttctc ccatactagc ttgccagtca
     4381 ggacgtcaag gcaatacagg gcgtttttac cggaattgtt ttcgttgtcg tcacggacct
     4441 tgccgcagcc aaaataaagc tttccctgat aaatagttgg cgatgtcctt atctctccgc
     4501 cagcctcaaa agaccagagc tctttgccag tttttgcgtc gaggcaatag agtttcttgt
     4561 caaatgacgg cgcaaacagt tttccctgcc agtaaaccgg cgtgtaggca agcgcccccg
     4621 atttgaactc ccagatcttt gcgcccgtct caagatccat gcaatggaac ttgccgtcct
     4681 gggcgcccgc aaaaaccatc ccatccacag aaattgcttg ggtggtgaga ggcgattttg
     4741 cattgtattt ccacaatatt ttcagcttgt cggtttttgg accgcaatcg gcctctgtgg
     4801 atgaagtcct gctcgggtca ccgccaaaac atggccagtc gcaaccctgc ttttcaaggt
     4861 tcagcccctc gtcatagaca tagacttttg cccttgcaga gagctggccg gaagtggcaa
     4921 caatatcagt cagccctggc gatttggcac caaagagacc gccataatca agcgttccgg
     4981 ttttggggtc cgtcaaagac caggttattt tctggtcttt catgacctca tcataattgt
     5041 tgtagactgt ggcagtaaaa agtatgctct ggccaggttt cagcacggca aattttggct
     5101 ttatggtcat tttaactggt ttttcaacac gcgcttcaag gcaataaacg ttgctgtcgt
     5161 ttgacccaaa atagatcttt ttattgcaga ttacaggtgt tgaccacacc tcatcctttg
     5221 tttcaaaaga agacagctcc ttgccagttt ttgcatcaag gcagtagagc ttattgtcat
     5281 aggagccaaa atatatcttc cctgcacaat atacaggtga tgagttgttg atttttccgt
     5341 ctgttttgta tttccaggaa agcttttttg atgatccgtc aacgcagtat aggttgccat
     5401 catcactgcc gaaatagatt ttaccctcgg caattgttgg tgttgtaagt atcggccctc
     5461 cagcaaggta ggtccagaga gttttgccag tcttcaaatc gaggcagtat agctttttgt
     5521 cccagttccc agcgtatatc ttgccggaag caactgatat tgatgctggg gcatcaaatt
     5581 cactgcctac ccttgcagat ttccagagct gtttgccatt tgtcgcatca aaacaataca
     5641 tgcaattgtc gtttgatcct atgacaattt tcccgccagc aattgctggg gaagcctcaa
     5701 aacccacctg gttttttacc ttccagatgg cttttcccgt tttggcatcc aggcaataaa
     5761 tgtaattatc acccttgttt gtcttgtcat ttgttttgcc gcaggctgca tagagcttgc
     5821 ccaaataaac caggggattg gagatgcttg cggatgacat tgtaaaaacc cagagctgct
     5881 tcccagtttt tgcatcaagg caatagaccc ttttgtcggt gcaggaagca taaaccctgt
     5941 ctgaatcata acatatcccg ttaaggaccc cgcctatctt tgctgaccag gagagagtcc
     6001 ctttctctgc atctaggcaa tacatcttgt tgtcccacga agccacataa acctttccag
     6061 agacaacgag tggtgcacag gtaacagaac ctcctgtcat atatgaccag cgctttacaa
     6121 ggtcattgtt ttctggcgca cactcagaag ggaaaacccc gttattgcca aggtcgaact
     6181 tgaaccttgt gcaatcacag ctggcagcaa cgccagggcc agaaacaaaa acagacgaca
     6241 tgacaatcaa aagcgcaagc gcccttgcag ttattttttt catttatact ccactatacg
     6301 ctagagggat gtattttgcc tgtatgtttt ttaacagcca ggatccacaa aatgcttttt
     6361 agatgattcc atggcaacat caaggtcatc taaggcttgc gacatctctt cggccagatt
     6421 ttttggcaag acatttagat cgattgcctt ttttatggcc tcagcggcat agtaagctgc
     6481 atcaaaaagc cttttgtgtg ctatttcaag tttttccata atgcctcctt ttggtaatta
     6541 taaccaaaaa ggggctggct tgatatcact taatcataat tgttactggg agcacgacaa
     6601 ctctgtcatt ccacgcaatt tttacaagcc cattgttcgg gcccttctgg gctttttgcg
     6661 gatcaattga aatattaaaa ttcacctttt cgccactctt catctcaaag gttgcaggtt
     6721 tt
//
LOCUS       DAOSRI010000071         6598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10739_length_6598_cov_4.012380, whole genome shotgun sequence.
ACCESSION   DAOSRI010000071 DAOSRI010000000
VERSION     DAOSRI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_6598_cov_4.012380"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1052
                     /locus_tag="PK717_03095"
     CDS             <1..>1052
                     /locus_tag="PK717_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOR46681.1"
                     /translation="KEEVKNLTIIIDPLTNLRSGPSTKSPVVNRTTSTIRPSVVAKSK
                     DADGKIWYEIVLPTGTHAWVRSDLAKTATPILEESVSTQKGIISKNVNIRKDAGLNFA
                     KVHTTKSQIVVQILSKKSDVNGKMWLKVQGDFGVGWVLGSMVETTSSSPFDIVAAKAQ
                     LRLAPSQGGKLLGVVPRESKCTISGSALNSDGGTWYLLTTQQGETGWVTSTKVKTSGS
                     VDLPPANLLGLTVELQKESTLASFPSGSGGPKIFAGGKGKVESVAQREGGRLYYRILS
                     GNQAGWVSSEDTKKHTQPQPVKPVDVGEIQWAKRGDVLKFTIPISSKPNSLNIKSYND
                     NPRIQLFLDNSLYTGD"
     assembly_gap    1053..1062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1063..2513
                     /locus_tag="PK717_03100"
     CDS             <1063..2513
                     /locus_tag="PK717_03100"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR46682.1"
                     /translation="EPIVVDSTLVSRVGVEQKSVSPPTVLVVFQLNKSCDYHLSPLNP
                     TSTELVLEIFEKVESNQMRIEIQGRPVHASDPPILLDNKVMVPIGALASHLGYIISWD
                     DKENRAEMSVDPKKPFVFSFTNLKPSMHVVDGQNSFYKTIAPAPRIVGSAFYVPIEPV
                     APMLGFSYMYSPVQNKVYLDPVIDKLEIGGCLDTSKACSTLSTQVSFFTDFDVTELPD
                     GRSKITLNNCVLGENALGGIDAKKVSVDFDPRTNATPPKVTILITKKSTEEIGYGEAR
                     GPNKLIFTLKEKNKSGLNGKTIIIDPGHGTFNEDGSYDVGCSGMMGTYESEVNLQTAF
                     ELGKMLSSQGATVIYTRTEERNIKTPDLEGRANLANSSGADMFISIHFNAARDPDAQG
                     TETYYYTDLGRRLAEKIHKNVLSKVGFEDRGIKKRGFYVCREIVSIPSILIEPLFLSN
                     KKGEGWIREEVNVKKLAEGIQLGIKEFFQEPI"
     gene            2510..3214
                     /gene="rph"
                     /locus_tag="PK717_03105"
     CDS             2510..3214
                     /gene="rph"
                     /locus_tag="PK717_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOR46683.1"
                     /translation="MRIDNRDNDQLRPVKIEPNYMKYAEGSALVVWGETKVICTASWE
                     PRVPNWLKELGQGWVTAEYSMIPRCSPQRIQRDINKKASRSVEIQRLIGRSLRSVVDL
                     QQLGENSITIDCDVIQADGGTRVASIVGGFVSLVLALRNIGLKKMPIKDYLGAVSLGI
                     IDNELRLDLNYEEDSRAQVDMNFVMDSAQKIAEVQATAEISTFPFDTFNKMLELGKKG
                     VNEIIELEKKILGPLQ"
     gene            3211..3792
                     /gene="rdgB"
                     /locus_tag="PK717_03110"
     CDS             3211..3792
                     /gene="rdgB"
                     /locus_tag="PK717_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOR46684.1"
                     /translation="MKILFATSNPGKLREAQAILTKVELVLASDYKVEMPEETGSTFE
                     ENARLKSEAGFTQTGIPTLAEDSGLVVEALDGRPGIYSARYAGGPEANNIRVLKEMEG
                     VANRRAKFVCVASYTDQNGTRTFTGELKGEIATTPKGSNGFGYDPIFLFNGEKTTAEI
                     TPEEKDRISHRGKALKAFQDWFISQASGEPTPW"
     gene            complement(3755..4336)
                     /locus_tag="PK717_03115"
     CDS             complement(3755..4336)
                     /locus_tag="PK717_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOR46685.1"
                     /translation="MTSSSNLLLHCCCGPCATASIDRFVQLGYKVVPYFFNPNIEPED
                     ERKRRLGAFLAVCQSKMLTPIVEDDPDYSHKRGLDRCFSCYSVRLEKTKLVARRLGLN
                     YFSTSLLISPYQKHELIKQVGSGLEGFVYEDLRPFYKDSQQQAKELGVYRQKYCACLE
                     SLEESKWLAKRKSEMKRQKSGPTKEWVPRWLAK"
     gene            complement(4320..5357)
                     /gene="ruvB"
                     /locus_tag="PK717_03120"
     CDS             complement(4320..5357)
                     /gene="ruvB"
                     /locus_tag="PK717_03120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR46686.1"
                     /translation="MEDRIIDPNIAQEDSDEVSLRPSRLSQFIGQERVKKNLQTFLEA
                     AKTRDPKKLGKGEGILDHILFYGPPGLGKTTLAVIVASELGVSLKRTTGKALERAGDV
                     AAILTGLSEGDILFVDEIHRMNPQIEETFYSAMEDFRFDIVLGKGPSARTITLPLNRF
                     TLIGATTRIRLPSSPLRDRFGIIERLEYYTPPELAEVAKRASKALGVEIDKELAHLLA
                     KRARGTPRIVLRLLRRARDFAVVSGTNVIGKEVLLETFKALEVNDYGLDKMDRRLLET
                     IIERFSGGPVGLETMAAILGEDRDSVEDVIEPYLLQEGMIERTPRGRVVLEKGYIACG
                     LKPRVKDDLLF"
     gene            5436..6338
                     /gene="nadA"
                     /locus_tag="PK717_03125"
     CDS             5436..6338
                     /gene="nadA"
                     /locus_tag="PK717_03125"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOR46687.1"
                     /translation="MDTSALQSEIRKIAKEKGIFILAHSYQPMEIQEIADYVGDSLDL
                     SKKAMESGSKKIMFCAVKFMAETASVLSPEKKIMIAIEEAGCPLANMITPEDVVEMKK
                     KYPKAKVVCYINSPIEVKALSDVICTSANALKIVEKIDSDEIIFIPDQGLGSWIASKT
                     KKTLHIFNGYCPIHWLMDADQVRTTKAYHPDALLVSHPESNPKVRELSDHICGTSGMI
                     TYVKENPGRCFIIATETGMKQRLEKMFPGERFVLATKNITCPNMKKTTLEKLLESLRE
                     EKHVITVDKEIADKARIAIERMHG"
     gene            6331..>6598
                     /locus_tag="PK717_03130"
     CDS             6331..>6598
                     /locus_tag="PK717_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOR46688.1"
                     /translation="MVKIPRYIAGSSAKENWEFDAIVVGSGIAGVIATMRLSREMRVA
                     LITKERLVDSNSFYAQGGLAASLGEGDSLELWKSDTIKAGDGLCR"
BASE COUNT         1949 a         1574 c         1563 g         1502 t
ORIGIN      
        1 aaaggaagaa gtcaaaaacc ttacaataat cattgatcca ctcacaaacc ttaggtccgg
       61 accatcaaca aaatcccccg ttgtcaacag gacaacgtca accatcaggc catcagttgt
      121 ggccaaatca aaagacgcag atggcaaaat ctggtacgag attgttcttc ccacagggac
      181 ccacgcatgg gtgagatcag atctcgccaa gacagcgaca ccaatacttg aagaatcggt
      241 ttcaacacaa aagggcatca tatcaaagaa cgtaaacata agaaaagacg ctggcctgaa
      301 ctttgccaaa gtccatacga caaagtcaca gattgtggta caaatactgt caaaaaaatc
      361 cgatgtaaat gggaagatgt ggttgaaagt tcaaggagat tttggggtgg gctgggtcct
      421 tggatcaatg gttgaaacaa catcttcaag tccgtttgat attgtggcgg caaaagcaca
      481 gctcaggctt gccccaagcc aaggcggaaa attgcttggc gttgtcccac gggaaagcaa
      541 atgcacaata tcgggttctg cgctcaactc agatggtgga acgtggtatc tcctgacaac
      601 ccagcaaggg gagacaggat gggtaacaag cacaaaagta aaaacttccg gctctgttga
      661 cctgccaccc gcaaaccttc tcgggctcac ggttgagctc caaaaagaat cgacactggc
      721 atcgttccca tcaggaagtg gcggcccaaa aatttttgcc ggcggaaaag gtaaggttga
      781 atctgtagcg caaagggaag gcggcaggct ttactacagg atactctcag gcaatcaggc
      841 aggatgggtg tcttcggagg atacaaaaaa gcacacacag ccacagccgg tcaaaccggt
      901 cgatgtcggt gagatccagt gggcaaaacg cggcgatgtc ctcaaattta caattccaat
      961 ttcatccaaa ccaaactcgc taaacatcaa aagctacaat gacaatccaa gaatacagct
     1021 atttctagat aattcactct acaccggcga ccnnnnnnnn nntggagccg attgttgttg
     1081 attcaacact ggtttcgagg gtgggtgtcg aacagaaatc cgtttcacca ccaacagtgc
     1141 tggttgtttt ccagctaaac aagtcctgtg attaccacct ctctccattg aacccgacgt
     1201 caaccgagtt ggtgctagaa atcttcgaaa aagtcgagtc aaaccagatg aggatcgaga
     1261 tccagggcag gcctgtccac gcaagtgatc cgccaatact gcttgacaac aaggtcatgg
     1321 tgccgattgg tgcactggct tcccacctcg gctacatcat ctcctgggat gacaaagaaa
     1381 acagggccga aatgagtgtc gacccaaaaa aaccttttgt cttctcgttt acaaatctca
     1441 agccatccat gcatgttgta gatggccaaa attcattcta caaaacaata gcgccagccc
     1501 caaggattgt gggaagcgct ttttatgttc cgattgaacc ggtagcgcca atgcttggct
     1561 tctcatacat gtactcgccc gtacaaaaca aggtctacct cgacccagtc atcgacaagc
     1621 tggaaattgg ggggtgcctc gacacaagca aggcatgctc taccctgtca acccaggttt
     1681 cattcttcac tgattttgat gtcaccgaac ttcccgatgg aagatcaaag ataaccctta
     1741 acaactgcgt tcttggggaa aacgcattag gcgggattga cgccaaaaag gtctctgtcg
     1801 attttgaccc aaggaccaat gcaactcccc caaaggtaac aatcctgata acaaagaaat
     1861 ccacggaaga gataggctat ggtgaggcaa ggggcccaaa caagctcatt tttactctca
     1921 aggagaaaaa caaatcagga ctcaatggaa aaaccatcat aatcgatccc ggtcatggca
     1981 ccttcaatga agacggttca tacgatgtcg gatgcagtgg catgatgggc acttatgagt
     2041 ccgaggtgaa cctccagaca gcattcgagc tcgggaaaat gctctcttcc cagggggcca
     2101 ctgtaatata taccagaaca gaggagagaa acataaaaac cccagatctc gaaggaaggg
     2161 caaatcttgc gaattcctcc ggtgccgaca tgtttatctc aatccacttc aatgccgcaa
     2221 gggaccctga tgcccaggga acagaaacct attactatac cgatctgggc aggcgtcttg
     2281 cagaaaaaat ccacaagaac gtcctctcca aggttggttt tgaggacagg ggaatcaaaa
     2341 aacgtggttt ttatgtgtgc cgcgaaatag tatcaattcc atcgatcctc atcgagccac
     2401 tgttcctgag caacaaaaaa ggcgagggct ggataaggga agaggtaaac gttaaaaagc
     2461 ttgcagaggg aatacagctt ggcatcaaag aattcttcca ggagccaata tgagaataga
     2521 caacagagac aacgaccagc taagaccagt aaaaattgaa ccaaactaca tgaaatacgc
     2581 cgaaggatcg gcgcttgttg tttggggtga gacaaaagtc atctgcacgg catcatggga
     2641 accaagggtc cctaactggc tcaaggaact cggccagggg tgggtcacgg cagaatactc
     2701 gatgattcca aggtgctccc cccagaggat ccagcgggac ataaacaaaa aggcctcgcg
     2761 ctcggtggaa atacaaaggc tgatcggaag gtcactgcgt tccgttgttg acttgcagca
     2821 gctgggcgaa aattcaataa caatcgattg cgatgtaatc caggctgatg gcgggacaag
     2881 ggtcgcctcg atagttggcg gatttgtttc actcgttctt gccctaagaa acatcgggct
     2941 caagaaaatg ccaatcaaag actatctcgg tgcagtctcg ctgggtataa tcgacaacga
     3001 attgaggctc gatctgaact acgaagaaga tagcagagcc caggtggaca tgaattttgt
     3061 gatggattcg gcacaaaaaa tagccgaagt gcaggcaact gcagaaatct cgactttccc
     3121 gtttgacaca ttcaacaaaa tgctggaact cgggaaaaaa ggtgtaaacg aaatcatcga
     3181 gctcgaaaaa aagatactcg gcccactgca atgaaaattc tttttgcaac atcgaatcct
     3241 ggcaaattga gagaagcaca ggcaatattg acaaaagtgg agcttgtgct ggcatctgac
     3301 tataaagtgg aaatgccgga ggaaactggc tccacattcg aggaaaacgc aaggctgaaa
     3361 tccgaagctg gatttaccca gacagggata ccaacacttg ccgaagattc gggcctcgtt
     3421 gtggaggcgc tggatggaag gcctggcata tattcagcca ggtatgccgg tggtccggag
     3481 gccaacaaca tcagggtcct gaaggaaatg gaaggcgtgg caaacagaag ggcaaaattt
     3541 gtatgcgtgg cctcctacac cgatcaaaac ggcacgcgca ccttcacagg tgagctgaaa
     3601 ggcgaaattg ccaccacccc aaaaggatca aatggttttg gctacgatcc gatatttctt
     3661 tttaacgggg aaaaaacgac tgcggaaatt acacctgaag aaaaggacag gatatcccat
     3721 cgcgggaaag ccctaaaggc ttttcaggac tggttcattt cgcaagccag cggggaaccc
     3781 actccttggt agggcctgat ttttgccttt tcatctctga ttttcttttg gcaagccatt
     3841 ttgattcctc aaggctctcc aggcaagcgc agtatttttg tctgtaaact ccaagctctt
     3901 ttgcctgctg ctgtgaatct ttgtaaaatg gtctcaggtc ttcataaaca aaaccttcaa
     3961 gccctgatcc gacctgtttt atcagttcat gcttctggta cggggaaata agcagggatg
     4021 ttgagaaata atttaaacca agccttcgtg ctaccaattt tgtcttttca agcctcacgg
     4081 agtaacacga aaaacatctg tccaaacctc gcttgtgaga ataatccgga tcatcttcaa
     4141 caataggagt gagcattttt gactggcaaa cagccagaaa ggcaccgagt cttctttttc
     4201 gctcgtcttc cggttcaatg ttcggattga aaaagtaagg caccaccttg tagccaagct
     4261 ggacaaaacg gtcaatcgag gcagtggcac aggggccaca acagcaatgg agaagaagat
     4321 tagaagagga ggtcatcttt gactcttggt ttgagtccac aggctatata gcccttttcc
     4381 agaacaaccc ttccccttgg cgttctttcg atcatgccct cctggagcag atatggttcg
     4441 ataacatctt ctaccgagtc cctgtcttcc ccaagaatgg cggccattgt ttccaggccg
     4501 accggcccgc cagaaaaacg ctctatgatc gtttcgagaa gcctcctgtc catcttgtcc
     4561 aggccataat cattgacttc aagggccttg aacgtctcaa gcaaaacctc tttgccaatg
     4621 acgtttgttc ctgaaacaac agcaaagtcc ctcgctctcc tgaggagcct caggacaatc
     4681 ctcggcgttc cccttgccct ctttgccaga agatgggcaa gctccttgtc aatttcaaca
     4741 ccaagggcct ttgaggccct ttttgcgact tctgccagct ctgggggggt gtaatactca
     4801 agcctctcta taatgccaaa tctgtccctt aaaggggagc tcgggagtct tattctggtt
     4861 gtggcaccaa tgagcgtaaa tctgttcaac gggagggtta tcgttctggc cgatgggcct
     4921 ttgccaagga caatgtcaaa cctgaaatct tccatggcgg agtagaatgt ttcttcaatc
     4981 tgggggttca ttctgtggat ttcgtccaca aaaagtatgt caccttccga caaacccgtc
     5041 aggatggccg ccacatcacc agctctttcc agcgccttgc cggtggttct cttgagcgag
     5101 acaccaagtt cggatgccac aatgacggca agggtcgttt ttccaaggcc aggcgggcca
     5161 taaaaaagga tatggtcaag tatgccctcg cctttgccaa gtttttttgg gtctctggtt
     5221 ttggcagcct caaggaaagt ctgaaggttc ttcttgaccc tctcttgtcc aatgaattgc
     5281 gaaagcctgg acggccttaa tgatacctcg tccgaatctt cctgggcaat attggggtcg
     5341 attattctgt cttccacttc ataaaaagta tatttttata aacattgcct ttcaagactg
     5401 ttgaaaatca gatcaaatgt attaaattcg agtcaatgga taccagtgct cttcaaagtg
     5461 aaatccgcaa gattgccaaa gaaaagggca ttttcatact tgcccacagt taccagccga
     5521 tggaaatcca agaaattgct gattatgtcg gcgactcgct tgatctgtca aaaaaggcca
     5581 tggaatctgg tagcaagaaa atcatgttct gcgctgtaaa atttatggcc gaaacagcat
     5641 cggtgctttc accggaaaag aaaatcatga tcgcaattga ggaggcaggt tgcccccttg
     5701 caaacatgat aactccggaa gatgttgtag aaatgaagaa aaaataccca aaagcaaagg
     5761 ttgtctgcta catcaactca cccattgaag ttaaggcgct ctcggatgtc atctgcacat
     5821 cggcaaacgc tttgaaaatc gttgaaaaaa tagactctga cgaaataata ttcatcccgg
     5881 accaggggct tgggtcatgg attgcctcaa aaacaaagaa aacccttcat atttttaatg
     5941 ggtattgccc aatccactgg ctcatggatg cagaccaggt gagaacaaca aaggcctacc
     6001 atccagatgc gctcctggtt tcacaccccg aatcaaaccc aaaagtaagg gagttatctg
     6061 atcatatttg tggaacatcg ggcatgataa cctatgtaaa agagaaccct ggaaggtgct
     6121 tcataatcgc cacagaaacc ggaatgaagc agagacttga aaaaatgttt cccggtgaaa
     6181 ggtttgtcct tgccaccaaa aacatcacct gcccaaacat gaaaaaaacc acgctggaaa
     6241 aactcctgga atcactgcgg gaagagaagc atgtgataac agtcgacaaa gaaatagccg
     6301 acaaggcaag aatagcaatc gagaggatgc atggttaaaa ttcccaggta tatagcaggc
     6361 tcttcagcaa aggaaaactg ggagtttgac gcaatcgttg tcgggtccgg gatagctggc
     6421 gtaattgcca ccatgaggct ctccagggaa atgagggtgg cgctgataac caaagaaaga
     6481 ctggtcgact caaactcttt ttacgcacag ggaggcctcg ccgcctccct tggcgaaggc
     6541 gattctctcg agttgtggaa atcagacacc ataaaagctg gggatggcct ctgccgtg
//
LOCUS       DAOSRI010000072         6580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10773_length_6580_cov_4.310651, whole genome shotgun sequence.
ACCESSION   DAOSRI010000072 DAOSRI010000000
VERSION     DAOSRI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10773_length_6580_cov_4.310651"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PK717_03135"
     CDS             <1..1256
                     /locus_tag="PK717_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOR46689.1"
                     /translation="RIVVLDTKTALANLSHAFYGYPSRSLKLCGVTATNGKTTTTHMI
                     AQLLRGVDPKVGLVGTAGHYLPTGRIQPDISNPTTTPYPTVIDEYLSEMKGAGSKYAV
                     LELSSFGLECGRVIGLEFDAIGIGNITHCHHAFLHGTHENYVKIKIDSLDLLKPEGVA
                     VLNIEDQHYEDALSKVGGKKVITFGAKKGDIHIASFKPTLKGSEVMASIFGKTHTYRL
                     NVPSVANALNSLCAIGIVEALGVQSAPLLGLLEQMPEIPGRWNWIDECQPFTVAIDKA
                     NTPAALETVAEHMESVHPKRKIIITNNVGEGDPKAREKLAEIASRTCDLTIITYGVAK
                     GEDIDSTVNQFASFMEKHGGKYKIIKDRTEAIHWAINQAQEGDFIAILGRGDEDGMYI
                     RGQWFEVDDRVEAKKALKARGFECN"
     gene            1247..2584
                     /gene="murF"
                     /locus_tag="PK717_03140"
     CDS             1247..2584
                     /gene="murF"
                     /locus_tag="PK717_03140"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOR46690.1"
                     /translation="MQLTIKEIASLFGQELDGRDTEVAGFSIDSRATNPGEVFFAIKG
                     QNVDGHEFALQAQEKGAVLVVCEKPLQVGIPQVIVPSTEEAMIALGSYYKNKFDCQVV
                     GITGSAGKTTTKELTAQMLSARYQVAKNEGNRNTPIGLPMALKALENDTEIFVAELSG
                     SFMDEIPKLLRIVRPVVGVITNVGPSHLETLGDIDGVAKSKSQLIESLPVGGLAVLNA
                     DDPRCKEMSSKTRCKKVTFGFDPEADIQGRLIDGLITVKSCGKAYTFRPHQDSIHFMY
                     DALASMAVATEYGIPLKECVEVAADFKPVEGRGTIIINPTGVRIIDESYNANPVSMKE
                     TLRSLAAKPGRRFAILADMLQLGEDSEKLHQELGEFVASLRLDGVYLFGNLSKRLAQN
                     CPRSLHFETKRDLLDAISPRLSSGDWVLVKGSNGMGMKEVVRSLATHANKEER"
     gene            2581..3510
                     /locus_tag="PK717_03145"
     CDS             2581..3510
                     /locus_tag="PK717_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR46691.1"
                     /translation="MIEKNWIWFHDHFGKGEEHHHAITRFIAQVDTPFQHVEIGISPY
                     FGKMIIIDGDVQSSVVDEHVYHELIVHPALFSHPNPKSILILGGGEGATLREILKHKT
                     VERVVMCDIDRQAVDLFKTHLPEWHQNSFYDPRVELIHGDARAYLEKAPDEEFDVIIS
                     DLTEPYSGGPSQRLFSVECYEEMARVTGPDGVYSAQGSLLRLQTYEVHLVIKTTCQTA
                     FPYARSCAGYIQSYDTPWSFITATKKQIPPIDNRIDELIAERINGDLKFYDQEAHNHA
                     FSLPKDIRALLSKKIDPITVSKPFSRESKSIDM"
     gene            complement(3586..4899)
                     /locus_tag="PK717_03150"
     CDS             complement(3586..4899)
                     /locus_tag="PK717_03150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR46692.1"
                     /translation="MRILDIAKMALSFASGDQVDVLVHHNDYSLTRFANSYIHQNVKE
                     NNIDVTVRVVRDNRQGIAQTNIVDPDSLRDVAQKAGQIADLSPKNPVDIKLPKPAEYI
                     ETGKPDEATASLTPEVRADAVAQMVNYANPLGLTLAGAYSSGSGSLAIVNSNGLEAYN
                     EFADAELKVTAMSEDSSGAAGFWTPRVSEISPLDIVKEAAGRAISSKKPAEIEPGEYE
                     VVLLHYAVAELISMMNWTGISARAAEEGRSWLANNIGKKILSDKITIVDDPFDPRSSM
                     RSFDFEGFPKSKMVLVDKGVPVDVVYDSYYATKTGHKNTGHAIPASSGAHPLALNVVV
                     EGGTSTVDEMVSKIKKGVLVSRFWYTNIMRPLEQEFTGMTRDGLFLIENGKIVSGLKN
                     MRVTDSIIRIFAGVEEVENVTRYHDGIVAPAMRSKGIIFSSKTKF"
     gene            complement(4896..6335)
                     /locus_tag="PK717_03155"
     CDS             complement(4896..6335)
                     /locus_tag="PK717_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR46693.1"
                     /translation="MKDLVERALKKARLEGASYGDIRLITETSEHVSAKDGVIKAIVS
                     DSSVGFGIRVIADGAWGFSSSSTVTPDEVDKVAAEAVAIAKASAKVTHDPVKLAEIDI
                     HVDDVPSGVNDDPRKVPLEKKVALALDATRSMEAQGVIARNASIHNSYQEKYFGSTEG
                     AMIRQEKIESGAGMEAYASDGKTFVKRSYPQSFGGDFARLGWSFVENMDLPGNGKRIS
                     QEVLALLKAKPCPKGKKDIVIDGAQMALQVHESLGHPTELDRVFGTEAGYAGKSFMMP
                     DMLNKLQYGSKIVNITADATIPGAFGGFAYDDEGVKAKRVFLVKDGLFVGYLDSREDA
                     AKLGMKPMGAMRASSWARIPIVRMVSVNLEPGNLTFDQLIGGVQDGIFMATTLSWSID
                     DHRLNFQFSAEIGWEIKNGKLGEMIRTPSYTGITPEFWNSCDGIGNRDLWHVWGVPNC
                     GKGEPAQIMHVSHGTAPARFRNIEVGVGS"
     gene            complement(6352..>6580)
                     /locus_tag="PK717_03160"
     CDS             complement(6352..>6580)
                     /locus_tag="PK717_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46694.1"
                     /translation="VTLVTFELGSTKIVLYIGKNTATVTVAGQSRAVKLDAPPVIKNG
                     VTMVPFRFVAENFGAKVSWDGVTKTARMVYP"
BASE COUNT         1777 a         1646 c         1518 g         1639 t
ORIGIN      
        1 acaggattgt agttctagat acaaaaacag ctctggcaaa tctttcacac gctttctacg
       61 gctatccatc caggagcctg aagctttgcg gcgtgacagc aaccaatggc aagacaacca
      121 ccacacacat gattgcacag ctcctcaggg gggtcgaccc aaaagttggc ctggtgggaa
      181 ctgcaggtca ttatctccca actggcagaa tacagccgga cataagcaat ccgacaacaa
      241 ccccttaccc aacagtcatc gatgaatacc tctccgaaat gaagggggct ggctcaaaat
      301 atgccgtgct ggagctttca agttttgggc tcgaatgcgg aagggtcatc ggccttgaat
      361 tcgatgccat cggcattgga aacataaccc actgccacca tgccttcctt cacggcacac
      421 atgaaaacta cgtcaaaata aaaatcgatt cacttgacct tttaaaacct gaaggtgtcg
      481 cagtgcttaa catcgaagac cagcattacg aagacgcact gtcaaaagta ggggggaaga
      541 aagttatcac atttggtgca aaaaaaggcg acatccacat tgcaagtttc aagccaaccc
      601 tgaaaggttc agaggtaatg gcgagcatct ttggaaaaac ccacacatac agactgaatg
      661 tgccaagtgt tgccaatgcg ctcaattcgc tctgtgccat agggattgtc gaagcgttgg
      721 gggtccagtc agccccactc cttggtttgc tcgagcagat gccggagatt ccaggcaggt
      781 ggaactggat tgatgaatgc cagccattca ctgttgccat cgacaaggca aacacacctg
      841 cagcgcttga aacagttgca gagcacatgg aatcagtgca tcctaaaaga aagatcatca
      901 tcacaaacaa cgtcggcgag ggtgacccaa aagcaaggga aaagctggcc gagattgcat
      961 ccagaacatg cgacctgaca ataatcacct acggcgtggc aaagggtgag gacattgact
     1021 cgacagtcaa ccaatttgca tcattcatgg aaaagcatgg tgggaagtac aaaataatca
     1081 aagacaggac ggaggccatc cattgggcca taaaccaggc ccaggaaggt gacttcattg
     1141 ccatacttgg aagaggggac gaagatggaa tgtacatcag gggccaatgg tttgaggtag
     1201 acgacagggt cgaggccaaa aaggctctga aagcaagagg ttttgaatgc aactgacaat
     1261 aaaagagata gccagtcttt ttggccagga gttagatgga agggacaccg aggtggcggg
     1321 gttctccata gattcgaggg caacaaaccc aggtgaagtg ttttttgcca tcaagggcca
     1381 gaatgtcgat ggtcatgaat ttgcattgca ggcccaggaa aaaggtgcag tgcttgttgt
     1441 ttgcgaaaaa ccgctccagg ttggtatccc gcaagtcatt gtcccatcca ccgaagaggc
     1501 catgatagca cttggctcgt actacaaaaa caagttcgat tgccaggtag tcgggataac
     1561 gggttctgcc ggaaaaacca ccaccaagga gctgactgcc caaatgcttt ctgctcgata
     1621 ccaggtggcc aaaaacgagg gcaaccgcaa tacccctatc ggactcccga tggcgctgaa
     1681 agcactcgaa aatgacactg aaattttcgt agcagagctt tctggttcgt ttatggacga
     1741 aatacccaaa cttctgagga tagtgagacc agttgttggt gtcataacaa acgttggccc
     1801 atcacacctt gaaacgcttg gcgacattga cggcgtggca aaatcaaagt cccagctcat
     1861 tgaatcattg ccagtgggcg gattggcggt cctgaacgca gatgacccaa gatgcaaaga
     1921 aatgtcatca aaaacaaggt gcaagaaggt aacatttggc tttgaccctg aagctgatat
     1981 tcagggaagg ctgattgacg gcttgataac agtcaaaagt tgcggaaaag catatacctt
     2041 cagaccacac caggactcca tccacttcat gtatgatgca cttgcatcaa tggcagtggc
     2101 aaccgaatac ggaatcccac tgaaagagtg cgttgaggtg gccgccgatt tcaaacctgt
     2161 cgagggtagg ggtacaatca tcatcaatcc taccggagtg agaatcatag acgaaagcta
     2221 caacgcaaat ccagtttcaa tgaaagagac ccttcgttcc cttgctgcca aaccagggag
     2281 aaggtttgca atactggcag acatgctcca gcttggtgaa gattcggaaa agctgcacca
     2341 ggagcttggc gagtttgttg catcactacg ccttgacggg gtctatttat tcggtaatct
     2401 gtcaaaacgt ttggcccaaa actgccccag gtcactgcat tttgaaacaa aaagggacct
     2461 gctggatgct atttcaccgc gtttatcaag cggtgattgg gtcctagtca agggttcaaa
     2521 cggcatggga atgaaagagg ttgtccggtc tctggcaacc catgcaaata aggaggaaag
     2581 atgatagaga agaactggat atggttccat gaccacttcg ggaagggcga agagcaccat
     2641 catgcaatca ctagatttat agcccaggtt gatacgccat ttcagcatgt tgaaataggc
     2701 atttcgccat actttggcaa gatgatcatc attgacggtg acgttcagtc gtcagttgtt
     2761 gatgagcacg tttatcacga gctcattgtt caccctgccc ttttctcaca cccgaaccca
     2821 aaatccatat taatccttgg tggtggcgaa ggcgcaacac tgagggaaat cctgaaacac
     2881 aaaaccgtag agcgcgttgt catgtgcgat attgacaggc aggctgtcga tctcttcaag
     2941 acacaccttc cagaatggca ccagaactca ttttatgatc caagggtcga gctcatccac
     3001 ggagatgcaa gggcctatct ggaaaaagct ccagatgaag aatttgatgt gataatttct
     3061 gatctcaccg aaccatattc tggcggtcca tcccagagac ttttctctgt cgagtgttat
     3121 gaagaaatgg caagggtgac tggcccggat ggcgtctatt cggcacaggg ttcactcctc
     3181 aggctccaga cttatgaagt tcaccttgtc atcaagacaa catgccaaac tgcattccca
     3241 tatgcaagat catgcgctgg gtacattcaa tcctacgata cgccctggag ctttataacc
     3301 gcaaccaaaa agcaaatccc tccaattgat aacaggatcg atgaacttat agccgagaga
     3361 atcaacggcg atctgaagtt ctacgaccag gaagcccaca accacgcttt ctctctccca
     3421 aaggacatca gggccctgct ttcaaaaaag atcgatccaa taactgtcag caaaccattc
     3481 tcccgcgaat caaaatcaat agacatgtaa ttttcagcaa acaaccgaaa caattaaaaa
     3541 gccccgtggt tgaaatcacg gggcttttgt tttatacaga attttttaga attttgtttt
     3601 gctggagaaa attatcccct ttgacctcat tgccggagca acaatgccat catggtacct
     3661 tgttacattc tccacctctt caacaccggc aaagatcctg attattgagt cagtgactct
     3721 catatttttg agtccggaga caatctttcc gttttcaatc aggaaaaggc catcccttgt
     3781 cattccggta aactcctgct cgagcggacg catgatgttc gtgtaccaga accttgaaac
     3841 cagaacacct tttttgatct ttgaaaccat ttcatcaaca gttgaagttc caccctcgac
     3901 aacaacattg agtgccagtg gatgtgctcc acttgatgct ggaattgcat ggccagtatt
     3961 tttgtggcct gtctttgttg cgtagtacga atcatagacc acatcaaccg gaacaccctt
     4021 gtcgacaagg accatctttg atttcgggaa accctcaaaa tcaaaagacc tcatcgagga
     4081 ccttggatca aacgggtcat caacaatggt tatcttgtcg gaaagtatct tcttgccaat
     4141 attatttgca agccaacttc tgccctcttc ggctgccctg gcagaaatgc ctgtccagtt
     4201 catcattgaa atgagttcag caacggcata gtgcaaaagg actacttcgt attcgccagg
     4261 ctcgatttca gccggtttct tgctgcttat cgccctgcca gcggcctctt ttacgatatc
     4321 gagggggcta atttcactga cccttggggt ccaaaaacca gcggcccctg aagaatcttc
     4381 tgacattgcc gttaccttca gttcggcatc ggcaaattcg ttgtaggctt caaggccatt
     4441 actgttgaca atcgcaaggc tcccgctgcc agaagagtag gcgcctgcaa gcgtaaggcc
     4501 aagcggattg gcataattga ccatctgtgc cacagcatcg gccctcactt caggagtcaa
     4561 ggacgctgtt gcttcatccg gtttcccagt ttcgatgtat tcggctggtt ttggaagttt
     4621 tatatcaaca gggttctttg gggagagatc ggctatctga ccagcctttt gtgcaacatc
     4681 cctcaaggaa tcagggtcga cgatgttggt ctgtgcgatt ccctgacggt tgtcccttac
     4741 aaccctgact gtgacatcaa tattgttttc cttgacgttc tggtggatgt atgaatttgc
     4801 aaatctggtg agtgaatagt cattatggtg taccagcacg tccacctggt caccgctggc
     4861 aaaagaaagg gccattttgg caatatccag tattctcatg aacccacccc cacttctata
     4921 ttacggaacc ttgctggagc agttccatgc gaaacatgca ttatctgggc tggttcaccc
     4981 ttaccgcagt tcggaacacc ccaaacatgc cagaggtccc tgttgccaat gccatcacag
     5041 gagttccaga attctggtgt gattcctgta tagcttgggg ttcttatcat ttcaccaagc
     5101 tttccgttct ttatttccca gcctatttct gccgagaact ggaagttgag cctgtgatca
     5161 tcgattgacc agctgagcgt tgtggccatg aaaatgccgt cctggactcc gccaatgagc
     5221 tgatcaaaag tgaggttgcc aggttccagg tttacagaaa ccatacgcac aattggtatt
     5281 ctggcccagc tgctcgccct catggcaccc atgggtttca tgcccagctt tgcggcatct
     5341 tcccttgagt cgaggtagcc aacaaagagc ccgtctttta caaggaaaac tctcttcgcc
     5401 ttcacaccct cgtcgtcata ggcaaaaccg ccaaaagcgc cgggaatagt cgcatcagca
     5461 gtgatattca caatctttga accgtattga agtttgttga gcatgtccgg catcatgaaa
     5521 gatttgccag catatcccgc ttcagtacca aaaaccctgt ccagttcagt tggatgacca
     5581 agtgactcgt ggacttgcaa cgccatttgg gcaccgtcta tgacaatgtc cttcttccct
     5641 tttggacacg gttttgcttt cagaagtgca agcacttcct gcgagatcct tttgccgttt
     5701 cccggaagat ccatgttctc gacaaaactc cagccaagcc ttgcaaagtc tccaccaaaa
     5761 gattgtggat aagacctctt tacgaaagtc ttcccatcag aggcatacgc ttccattccg
     5821 gcccctgatt caatcttttc ctgccttatc atggcacctt cggtggatcc aaagtatttt
     5881 tcctggtagg aattatggat gcttgcgttt cttgctataa caccctgagc ttccattgat
     5941 cttgttgcat caagggcaag cgcaaccttc ttttcaagtg gtactttcct tgggtcatcg
     6001 tttactccgg atggtacatc atcaacgtga atgtcaatct cggccagttt gaccgggtca
     6061 tgcgtgacct tggcagaagc ctttgctatt gcaacagctt ctgcggccac tttgtcgact
     6121 tcgtcagggg tcaccgtcga tgaggaggaa aatccccaag caccatcagc tatgaccctt
     6181 atcccaaaac caaccgaact atcggaaaca atggccttta tgactccgtc ttttgcgctg
     6241 acatgttcgc tggtttcagt tatcagcctg atgtcgccat aagaggcccc ttcaaggcgg
     6301 gcttttttta atgccctttc aacaaggtct ttcaacgtat cgtcctcctt atcatggata
     6361 taccattctg gcagtctttg tgacaccatc ccagcttacc ttggcgccaa agttttcagc
     6421 aacaaacctg aatggcacca ttgtaacacc gtttttgata acaggaggcg catcaagttt
     6481 gactgctctg gactgccctg caactgtaac ggtagcagtg ttcttgccta tgtaaagaac
     6541 gatttttgtt gaaccaagtt caaacgtaac aagtgttacg
//
LOCUS       DAOSRI010000073         6559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10821_length_6559_cov_3.332257, whole genome shotgun sequence.
ACCESSION   DAOSRI010000073 DAOSRI010000000
VERSION     DAOSRI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10821_length_6559_cov_3.332257"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK717_03165"
     CDS             <1..307
                     /locus_tag="PK717_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46695.1"
                     /translation="DVMTHGIIRPVVAIIPSRTNEYWVQTFTDNMSEVPMIINASSLD
                     AYDIEGYTWMSPKELSPTNASFVLSSPKPEQALELSMETQAQSWMRALPKYLFEMEV"
     gene            304..747
                     /gene="rimI"
                     /locus_tag="PK717_03170"
     CDS             304..747
                     /gene="rimI"
                     /locus_tag="PK717_03170"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOR46696.1"
                     /translation="MIEIRRAKPLDFEKVYEIECLVKKQPWGMLDFYDDFINNPRSIW
                     YIAEKNGEIIGFGGMWRGVDEFHIVNLAITNPERGKGYGKELVKKMLEDAMSEKPNMV
                     LLEVRASNAVAKGLYIQFGFRELYVRKNYYQEDGEDGIIMVLESK"
     gene            744..1742
                     /gene="tsaD"
                     /locus_tag="PK717_03175"
     CDS             744..1742
                     /gene="tsaD"
                     /locus_tag="PK717_03175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR46697.1"
                     /translation="MISLGIETSCDDTSVGIVDQSGKILAQIRASQDRVHQVWGGIVP
                     ELASRAHLENILPVVDMAFRESMIHPDDVDIITCAYGPGLVGSLLVGIETAKALSLAW
                     NKPLIGVNHLRGHLAAVMLACPEMAMPSLGLLVSGGHSEVIYIEPDSTVKMLGETRDD
                     AAGETLDKFGRVLGLTYPAGPEIDRMTFIGDQNAIQFPVTKLKDGSMDFSFSGLKTAG
                     VRAIEKHPEMSKEDLASSFMKAVVDQLLDRLLLAIEKTEAKSIFIVGGVASSAYLKER
                     VTKLGDSVGLPVFVPPPSLCTDNGSMIAYCGFLYQQFGRKSGLDLQPQSRLPITAM"
     gene            1796..2533
                     /locus_tag="PK717_03180"
     CDS             1796..2533
                     /locus_tag="PK717_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR46698.1"
                     /translation="MSNQVPPNKKKILVIDDEHRIVETITYNLRAEGYTVFTAATAEE
                     GLEQLRRRQPDLVVLDLMLPGMSGTDFLRIIRQESQVPVIILTAKTSEIDKVVNLKMG
                     SDDYMTKPYSFAELLARIEALLRRTNQAPKIAEERAPEEIRVGDLYMNYSKRECVING
                     KPVNLGYKEFEILWFLASNPDKVFRRDDIMSKVWGIEGIPLDTKTVDVHIRWIRKKIE
                     PEPENPKYIETVRGVGYKFSTHKASEN"
     gene            2611..4311
                     /locus_tag="PK717_03185"
     CDS             2611..4311
                     /locus_tag="PK717_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR46699.1"
                     /translation="MTFRDYYETELFNRMLFQADVLEPRLKSFILSGNPFGVSDFLKE
                     ESNKFPLLGLSLLDSNGKVVSSTWGQKGDDWSGFPEVASVLKRENGFNIRTFDFEKQA
                     FVATPIVSDQEIVGIIVMRSSMEPVSAGTSQVIRFIIGTFLIALLAGVLMVYSSVRIQ
                     TQPLQSLIESARSISLGNLDETFESRSSDEVGQLGRVLQIMVTRLKSALREAGEERSR
                     FQAIFSNMVDGLLLIDSNGKILLANNSFLTIFGVSFKDIIGIQVDNSVLPLQLSAIIL
                     RGEDGVDSEMILEKPAKRVLRIRTATIEQDGCAIGTLSICEDITRMKLLESSEQEFVQ
                     YISHELKTPLASLSASIETITTKAKHDPEAQAKFLGNMGEDVHRLTDLVNSILTYQRI
                     RDTGEQMFKFGAVEMVLDVHGRFLAYANKRKIHFDFEVPDDEVYVIANKDRIIQVLIN
                     FIDNAIRFTPEGGKVVVGIENTPDSRRVKFYVSDTGIGIPKEYIGKIGERFIKIPRKD
                     HKFDTQVGLGVSICKEILRRHGSPLEVASEEGKGTTFSFYLKRATQSNSREKSADLTE
                     "
     gene            complement(4269..5099)
                     /gene="nadC"
                     /locus_tag="PK717_03190"
     CDS             complement(4269..5099)
                     /gene="nadC"
                     /locus_tag="PK717_03190"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOR46700.1"
                     /translation="MEQLTIRNLVRKALEEDLAWGDITSSIMVPEDDMIGARLLCKSE
                     CVLCGEYPFTMAFEEVDPLIQCDFHIKEGTLIKPGETAARIFGPTKGMLMAERVALNF
                     LNHMSGIATQTRAVVESIEGTGCHVTDTRKTLPLLRGIQRHAVMVGGGTNHRMDLSSL
                     VLIKDNHIRAVGSVSKAVFLAKSKVSHVMKVEVEVTCASEAKEAFDAGADIVMLDNTT
                     PDQIKEIARMKPPHVILEASGKITPQNARDYAESGVDVISMGFLTHSVKSADFSLEFD
                     "
     gene            complement(5087..>6559)
                     /gene="nadB"
                     /locus_tag="PK717_03195"
     CDS             complement(5087..>6559)
                     /gene="nadB"
                     /locus_tag="PK717_03195"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOR46701.1"
                     /translation="SNSFYAQGGLAASLGEGDSIELWKSDTIKAGDGLCRESAVKMIE
                     EQTPLIIEELNQMGVPFDLDNEGHFILGREAFHSVRRIIHAGGDQTGKMISQTMVDQL
                     KTGSQATIFEEAFVVDILQDDNGEVAGVSAIIEDNDVMFSAPNVIIATGGSGQLYFYT
                     SNPRVATGDGMAMALRAGATLVDMEFVQFHPTVCFAIQGQPFLVTEAIRGEGAFLLND
                     KQERFMPRYHEKAELAPRDIVSRACFNEMKLSGSPWVYLDTTKFERGFFQKRFPTVYS
                     QCLKAGFDPTQQPIPVSPGAHYMMGGIETDLSCRTSLKGVYACGEAGSNGMHGANRLA
                     SNSIPEALVTGSLGAKSVLEDSRKAGPPERPFKPQYDREPYTRSELRLENWENIGLVR
                     EERKMERHLSKLRQGTLIVPESASLGSLELANMYTVTSIITMSSLERKESRGSHMRTD
                     YQAKDAIWGNLRVHTKAKLDGKHLEIEVGTSKKDGYSWNS"
BASE COUNT         1697 a         1488 c         1637 g         1737 t
ORIGIN      
        1 cgatgttatg acccatggaa taataaggcc cgttgtggca atcattcctt caaggacaaa
       61 tgaatactgg gtgcaaacgt tcacagacaa catgagtgaa gtacctatga ttatcaatgc
      121 cagctctctt gatgcttatg atatcgaagg atatacgtgg atgtccccaa aagaactttc
      181 accgaccaac gcgagctttg tgctgtcctc tccaaaaccg gagcaagcgc ttgagctttc
      241 tatggaaaca caggcccagt catggatgag ggccttgcca aaataccttt tcgagatgga
      301 agtatgatag agataagaag ggccaagccg cttgattttg aaaaggtcta tgagatcgag
      361 tgccttgtaa aaaaacagcc atgggggatg ctggattttt atgatgattt catcaacaat
      421 cccaggtcca tatggtatat tgcagaaaaa aacggggaaa taattggctt tggcggcatg
      481 tggaggggtg tggacgagtt tcacattgtt aatctggcaa taaccaatcc ggagcgtgga
      541 aaaggctacg gtaaagagct tgtaaagaag atgcttgagg acgccatgtc tgaaaaaccg
      601 aacatggtct tgcttgaagt cagggcgagc aacgccgttg caaaaggcct ttacatccag
      661 tttggtttca gggagctcta tgtgagaaaa aattactacc aggaagatgg cgaagacggg
      721 atcatcatgg tactggagag caaatgatat cactagggat tgaaacgtca tgcgatgata
      781 cctctgtcgg cattgtggac cagtctggca agattcttgc gcagataagg gcctcacagg
      841 acagggtgca ccaagtctgg ggtggaatag tgccagaact cgcctcgagg gcccaccttg
      901 aaaacatcct cccagttgtt gacatggcct tcagggaatc aatgatccat cctgatgatg
      961 ttgatattat cacctgtgcc tatgggccag gtcttgtggg gagccttctg gtgggcatag
     1021 agacggcaaa ggccctttct ctggcatgga acaaaccact tattggcgtc aatcatctga
     1081 ggggccacct ggctgctgta atgcttgcgt gtccagaaat ggcgatgccg tcgcttggtc
     1141 tccttgtttc cggtgggcat tcagaagtca tttatattga accggattct actgtcaaaa
     1201 tgcttggaga aaccagggat gatgccgccg gtgaaacact cgacaagttt ggaagagtgc
     1261 ttggtcttac ctatccagcc ggccctgaaa tagacaggat gacctttatt ggcgatcaga
     1321 atgcgataca gtttcctgtg acgaaactga aagacggaag catggatttc agtttttccg
     1381 gtctcaagac tgcaggtgtg agggccatag aaaaacaccc tgaaatgtca aaggaagatt
     1441 tggcttcatc ttttatgaag gctgttgttg accagctact cgacagattg cttctggcaa
     1501 ttgaaaaaac agaggcaaaa tcaatcttta tagtcggagg cgttgcttca agcgcatacc
     1561 tgaaagagcg tgtgaccaaa cttggtgact cagtcggact tccggttttt gttccccccc
     1621 cctccctttg caccgataat ggatcaatga tagcctattg cgggtttctc tatcaacagt
     1681 tcgggagaaa atccgggctc gatctccagc cacaatccag attgccgatc acggccatgt
     1741 gattcgttga caagttaaat ccgaaggtta aaatgatttt gaccaaggag gttaaatgag
     1801 taatcaagtg ccgccaaaca agaagaaaat tctggttata gacgatgaac acaggatcgt
     1861 cgagaccata acatacaacc tgagggctga aggctatacg gtttttacag ctgcaacagc
     1921 tgaagaaggt cttgagcagc tcaggaggag acagcctgat ctggttgtcc ttgacctcat
     1981 gcttcctgga atgagtggca ccgatttttt acgcataata cgtcaggagt cacaggtccc
     2041 tgttatcatt cttactgcaa aaacatctga aatagacaaa gttgtcaatc tgaagatggg
     2101 ttctgacgac tacatgacaa agccgtactc gtttgcagag cttcttgcaa ggattgaagc
     2161 cctattgaga cgcaccaacc aggccccaaa gattgccgag gagagggcac ccgaagagat
     2221 cagggttggc gacctctaca tgaactactc gaagagggaa tgcgtcataa acggcaaacc
     2281 ggtcaacctt ggttataagg aatttgaaat actgtggttc cttgcaagca atccggacaa
     2341 ggttttcaga agagatgaca ttatgagcaa ggtctggggt atcgagggca ttccgcttga
     2401 tacgaagacg gttgatgtgc atatccgctg gatccgcaaa aaaattgagc cagagccaga
     2461 gaatccaaag tacatagaaa ctgtgagggg cgtgggctac aaattctcca cgcacaaggc
     2521 atctgaaaat tgaacctttg ggttaggcta tttctaacct tccttatctg cctttcgctg
     2581 accctctcga tgttgggggt cttttttgcg ttgaccttca gggattacta cgaaacagaa
     2641 ctcttcaata ggatgctctt tcaagctgat gttttagagc cgcgcctaaa atcatttatt
     2701 ttgagcggca acccttttgg ggttagtgat tttctcaagg aagaatcaaa caaattccct
     2761 ttgcttgggc tttccctgct ggactcaaat ggaaaagtcg tatcgtcgac atggggacaa
     2821 aagggtgatg actggtctgg ttttccagaa gttgcatctg ttttgaagag ggaaaatggt
     2881 tttaatatca gaacttttga tttcgaaaaa caagcctttg ttgcaacccc cattgtatct
     2941 gatcaggaga ttgttggaat aatagtaatg aggagctcga tggagcctgt tagtgcaggc
     3001 acaagtcagg ttataagatt cattattggc acattcctca tagccttgct tgcaggtgtc
     3061 ctcatggttt attcgtcagt gcgcatacag acgcaaccgt tgcaaagcct cattgaatct
     3121 gcaagaagta taagcctggg gaaccttgat gaaacatttg aatcgaggtc aagtgatgag
     3181 gttggacagc ttgggagggt cctgcagata atggtgacca gactcaagag tgccctcagg
     3241 gaagcaggcg aggaacggag ccgctttcag gcgatttttt caaacatggt cgatggtctc
     3301 ctgctaattg acagcaacgg caagatattg cttgccaaca actcctttct cacgattttt
     3361 ggtgtttcgt tcaaggacat aatcggtatc caggttgata attctgtttt gccgcttcaa
     3421 ttatcggcaa tcatcctcag gggcgaggat ggggttgatt cagagatgat tctggaaaaa
     3481 ccagccaaga gggtcctcag gataagaacg gcgactattg aacaggatgg ctgtgccata
     3541 ggcacccttt caatatgcga agacattacg cgcatgaagc tgctggaatc atcagagcag
     3601 gagtttgtcc agtatataag ccatgagctg aaaactcctc ttgcatctct ttcagccagc
     3661 attgagacca tcaccactaa ggcaaaacac gatccagaag cacaggccaa gtttctgggc
     3721 aatatgggtg aagacgtcca caggctcact gatcttgtaa attcgattct cacttatcag
     3781 cggataaggg acactggcga acagatgttc aaatttgggg cagtcgagat ggtgctggac
     3841 gttcacggca ggtttctggc ctatgcaaac aaaagaaaaa tacactttga ttttgaagtc
     3901 cctgatgacg aagtttatgt tattgccaat aaggacagaa taatacaggt tttgattaat
     3961 ttcatcgata acgcgataag atttactcca gaaggtggca aggtcgttgt cggtattgag
     4021 aacacgcctg actcaaggag agttaagttc tatgtttcag atactgggat aggaatcccg
     4081 aaagaatata ttggaaaaat aggggaaagg tttatcaaaa ttcccagaaa agaccataaa
     4141 ttcgacaccc aggtggggct tggggtctct atatgcaagg aaatcttaag acgtcatggg
     4201 agtccacttg aagtggcgtc tgaagaaggc aaaggtacga cttttagctt ctacctcaag
     4261 agggcaactc agtcaaactc gagggaaaaa tctgctgatt tgacggagtg agtcaaaaag
     4321 cccatcgaaa ttacatcaac tcctgattcg gcataatctc ttgcattttg tggtgttatc
     4381 ttgccggagg cttcaaggat aacgtggggt ggtttcattc tggcgatttc ttttatctgg
     4441 tccggggtcg tgttgtcgag catgacaatg tctgctccag catcaaaagc ctcttttgcc
     4501 tcgcttgcgc aggttacctc gacttccact ttcatgacat gggaaacttt tgattttgcg
     4561 agaaagactg cttttgaaac agaccctacg gcccttatgt ggttgtcttt gataagcaca
     4621 agagaagata gatccattcg gtggtttgtg ccaccaccca ccatcaccgc atgcctctgg
     4681 attcctctta gtaaaggcag ggtttttctt gtgtccgtaa catgacagcc tgtcccttca
     4741 atgctctcca cgacggccct tgtttgcgtg gctatgcctg acatgtggtt caggaaattc
     4801 agggccaccc tttcggccat cagcatgccc tttgtcgggc caaatatcct tgcggccgtt
     4861 tcgccgggtt ttatcaaagt tccttccttg atgtgaaaat cacactgtat gagcgggtcg
     4921 acctcttcaa aggccattgt gaagggatat tctccacaga ggacgcattc cgatttgcac
     4981 aagagccttg cgccgatcat gtcgtcttcc ggcaccatga tggacgaagt gatatcgccc
     5041 caggcaagat cctcttcaag cgctttcctc acgaggtttc ttatagtcag ctgttccatg
     5101 aatagccgtc ctttttgctc gttccaacct caatctcaag gtgttttcca tcaagttttg
     5161 cctttgtatg aaccctcagg ttgccccata tcgcatcttt ggcctggtaa tcagttctca
     5221 tatgacttcc cctcgattcc ttcctctcca gggaagacat tgtgatgatc gaggtgacag
     5281 tgtacatgtt tgcaagctcc agggagccaa gggaggccga ttccggaaca atcagtgtgc
     5341 cctgcctcaa ttttgacagg tgtcgctcca tcttcctctc ttctcttacc agtccaatat
     5401 tttcccagtt ctcgagcctc agttctgaac gggtgtaggg ttccctgtca tactgcggct
     5461 tgaaaggtct ctctggcggg ccagcttttc ttgagtcttc gagtactgat ttggcgccaa
     5521 gggagccagt taccagtgct tccgggattg aatttgatgc cagcctgttt gccccgtgca
     5581 tcccgtttga tccggcttca ccgcaggcat atacgccctt taatgaggtc ctgcaggaaa
     5641 ggtctgtttc aatgccgccc atcatgtaat gggcaccagg tgaaacaggg atgggctgct
     5701 gtgttggatc aaatcctgcc ttgaggcact gggaataaac ggttggaaat cttttttgaa
     5761 aaaaccctct ttcaaatttg gttgtgtcga gatagaccca gggtgaaccg gaaagcttca
     5821 tctcgttgaa gcaggccctt gatacgatgt ctcttggggc cagttctgcc ttttcgtggt
     5881 atctgggcat gaacctttcc tgcttgtcat tcaagagaaa cgcgccctca ccgcgtattg
     5941 cttcggtcac cagaaatggt tgcccctgta ttgcgaagca aactgtcggg tggaactgca
     6001 caaattccat gtcaacaagc gttgcacctg cacgaagggc cattgccatt ccatcacccg
     6061 tggccacacg tggattgctt gtgtagaagt agagctggcc agacccaccg gttgcaatga
     6121 ttacattcgg tgccgagaac atgacatcgt tgtcttctat gattgcagaa acgccggcaa
     6181 cttcaccgtt atcgtcctgg agaatatcga caacgaacgc ctcttcaaaa atggttgcct
     6241 gtgagccagt cttgagctgg tcaaccattg tttgcgaaat catttttccg gtctggtccc
     6301 cgccggcgtg aattatcctt ctcaccgagt ggaaagcttc cctgccaagt atgaaatggc
     6361 cctcattgtc gaggtcaaac gggacgccca tctggttcaa ttcttctatg atcaggggcg
     6421 tttgctcctc tatcattttg acagcacttt cacggcagag gccatccccg gcctttatgg
     6481 tgtctgattt ccagagctca atagaatcgc cttcgccaag ggaggcggcg aggcctccct
     6541 gtgcgtagaa agagtttga
//
LOCUS       DAOSRI010000074         6494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_10959_length_6494_cov_5.019568, whole genome shotgun sequence.
ACCESSION   DAOSRI010000074 DAOSRI010000000
VERSION     DAOSRI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10959_length_6494_cov_5.019568"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK717_03200"
     CDS             complement(<1..457)
                     /locus_tag="PK717_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOR46702.1"
                     /translation="MSKVLVKQNILSWALNRSGKSREELKKDFPKIQQWFEGSEQPTV
                     KQLESFANKTSTPFGFFFLDEPPNDIIPIPYFRSLRNDVPKSPSPELIETIQTMQRRQ
                     SWLREYLIEEDFEKLSFVKSVSLNDDFIFIANEMKEQLGLAANWTSKLHN"
     gene            complement(701..1207)
                     /locus_tag="PK717_03205"
     CDS             complement(701..1207)
                     /locus_tag="PK717_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOR46703.1"
                     /translation="MVTQPFLLDSCVFIEAAKRYYAFDLAPGFWDKLMAHAKNGDLFS
                     IDRVKKELEKGRDELASWAKKDFSFAFASTNNQEIINQYSLLMNWANLNKQYRPEAKT
                     EFADDENADAWLLAYAKAKNITIVTEEQLSLGAKRKIPIPNVCKEFGIPYINTFGVLR
                     KLGVKLVV"
     gene            complement(1211..2356)
                     /locus_tag="PK717_03210"
     CDS             complement(1211..2356)
                     /locus_tag="PK717_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOR46704.1"
                     /translation="MSKVLVKQNILSWALNRSGKSREELKKDFPKIQQWFEGSEQPTV
                     KQLESFANKTSTPFGFFFLDEPPNDIIPIPYFRSLRNGVPKSPSPELIETIQTMQRRQ
                     SWLREYLIEEDFEKLAFVKSVSLNDDPVFIANKMKEQLGLAANWTSKLHNWEEALNLL
                     SASIENTGTIVAVNGIVGNNTHRKLQVDEFRGFVLVDDYAPLIFVNNSDSKAAQMFTL
                     AHELAHVFLGSSAAFDLREMQPADEPSEQICNKAAAEFLVPHQELHKIWNSIKKSNNP
                     PFQEIAKHFKVSELVVARRTLDLGLTTKKEFFDFYNDYITKNKQKPKQSGGDYYSSQS
                     GRIGRKFAQIIDRAVGEGKLLYSEAYRLTGVYGSTYEKYMMLVRDSR"
     gene            complement(2558..5200)
                     /locus_tag="PK717_03215"
     CDS             complement(2558..5200)
                     /locus_tag="PK717_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOR46705.1"
                     /translation="MRENEIKFDPFDEIPIEKRWFPDQEQLSQKEYDCLLPPLVEKIR
                     REIYKWRGQVYKGASQTTKSLLCHWFLETEGRELRYFFAQREAVETIIYLYEIIKIRD
                     KYGLAAFDSSGKLKVEEFEENWTRYCIKMATGTGKTKVLSLLLAWSYFHKLYEPGSMM
                     SKNFLVVAPNIIVLERLKRDFDNLCIFRNDPVIPENGYDGRNWKRDFCPELHIQDDIG
                     QISQNGNIFLTNIHRVYMNETLPTRETYWLGEKPVPNADRSGGMDLGKIIRSKQIHDL
                     VVFNDEAHHIHSKNLQWFEGIRDINSGLLQRTGKGISAQIDLTATPKHNNGEIFAQTV
                     SDFPLVEAIKCNIVKKPVLPDKESRDKLDVRQSDDAGEKYSDFIHLGYIEWKKHFDSL
                     KGKKTPVLFVMAEDTKEAEQIALHLEKNYQVMKNRVLVIHTNKTGEIDEKRSEKELQV
                     LRNAANSVDSSNSPYLAVVSVMVLREGWDVRNVTAIVGLRPFKAKSQILPEQAIGRGL
                     RKMFDLQTVEELVVVGNPPFIEFVESLKSEGVRLGYRTMGEKSDETGAPLIIELDRAN
                     LDKDLEKMDIVLPKLGSKYRWDPECIFSIDVDAIAFKPLKLDVLMRNDPKEIQFIDIL
                     GSWHHKIEFKPELVNFSNIIGFFTSAILKEARMPSGFNELYPKVEHFAKNRLFGKIME
                     IDDPVFLMNFTRPEVSKTIFDTFRAEIERLTLKQVENPRVDHGGHSQLDFEPIAVSSQ
                     RYVRSKKCLQNMVVGNQLELEFAAFLDRCQDVVSFARIYERRSRFRLDYATESGKIQN
                     YYPDFVAKTKDGFVYIAETKGIVDEDAKRKRARLEQWCKDANKSAGKESYRSLFIRQA
                     EWEKFRETLKKIEEANKIFGNMEN"
     gene            5690..>6494
                     /locus_tag="PK717_03220"
     CDS             5690..>6494
                     /locus_tag="PK717_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46706.1"
                     /translation="MPIYESQKEYDKLVIELKKDLCPHGTLEIVLVEKIANIIWKTRR
                     LARYENAHIRWDFSRVVDNILKDWVSWKERTSTNEDLAIDVGKSRLEIFGDEEMEISE
                     RLLSFVEFDDLQEDGSLEGKKIFLIINTLCDFEYLNRREKHEKIPCSSLEFFDVKELW
                     PDIFNENFSKTKKQIKEIISIIAKQFGKTQKELYESGHKLVLSLFEEYKLISESVGKE
                     MEVKIQQCLFDEYISENVVKLEAHLNRQLLQTLHELQRIQSMRVGISRPP"
BASE COUNT         1764 a         1331 c         1277 g         2122 t
ORIGIN      
        1 agttgtgcaa tttggaggtc cagttggctg ccaacccaag ttgttccttc atttcgtttg
       61 cgatgaaaat gaagtcatca tttaagctga cagattttac aaatgaaagt ttctcaaaat
      121 cttcttcgat caggtattcg cgcaaccaag actggcgtct ttgcatggtt tgaatagttt
      181 caataagttc tgggctcgga ctttttggaa cgtcattcct gagcgatcta aaataaggga
      241 ttggtataat gtcgtttgga ggctcatcaa gaaagaaaaa accgaaaggt gttgatgtct
      301 tattggcaaa cgattccagt tgctttacgg tgggttgttc actgccttca aaccattgct
      361 ggattttggg gaagtctttt ttcaactctt cccttgattt tccagagcga tttaaggccc
      421 aagataaaat attttgtttt acaagcacct tgctcattaa gtggcctcct ttccatattg
      481 attctaatca aaattatttc gaaatgaaag aagagattag ttcttttatg ggcctatgct
      541 agaggtttat gaattgagaa agatgatgat ttttcaaact atagctgttt atacagtttt
      601 tacccggtct tatcagttcg attatttcct gtttttcttt ttccgagagg ttcattttct
      661 agttctccag ttcgagtgat gcaatttttg ggttcttgtt ctaaactacc aatttcacgc
      721 caagtttcct cagcactccg aatgtattta tatatgggat tccaaattct ttgcagacat
      781 ttgggatagg aatttttctt tttgcaccca aactcagttg ttcttctgta acaattgtta
      841 tattttttgc ctttgcataa gccaaaagcc aggcatctgc attttcatca tcagcaaatt
      901 cagttttggc ttcaggccta tattgcttat ttaaatttgc ccagttcatt agtaatgagt
      961 actggtttat gatttcttga ttattagtcg aggcaaaagc aaatgaaaaa tctttttttg
     1021 cccatgaagc aagctcatca cggccttttt ctagctcctt ttttacacgg tcaatactga
     1081 aaaggtctcc attttttgca tgtgccatca acttgtccca aaacccgggg gccaaatcaa
     1141 atgcgtaata acgttttgca gcctcgataa atacacaaga gtcaagaaga aaaggttgag
     1201 tgaccattaa ttaccgggag tctctgacca acatcatata tttctcataa gtacttccat
     1261 aaacccccgt taatctgtag gcttcagaat agagcaattt cccctcccct actgccctat
     1321 caataatttg ggcaaatttg cggccaatac gaccgctctg gcttgagtag tagtctcccc
     1381 ccgattgttt tggtttttgt ttatttttgg ttatataatc attataaaaa tcaaaaaatt
     1441 ctttttttgt tgtcaacccc aaatctagtg ttctccttgc aacaacaagc tcgctcactt
     1501 tgaagtgttt tgcaatttcc tggaaaggag ggttattact cttttttatt gagttccata
     1561 ttttgtgcag ctcctgatgg ggcaccagaa attctgcagc agccttattg catatttgtt
     1621 ctgaaggttc atccgctggc tgcatttcgc gtaaatcgaa tgctgcactg ctgccaagaa
     1681 aaacatgggc cagttcatgg gccaaagtaa acatctgcgc tgctttgcta tcggaattgt
     1741 tgacaaaaat tagaggagca taatcgtcca ccaatacaaa acccctgaat tcatcgacct
     1801 gcaacttgcg atgtgtatta ttgccaacaa taccattgac ggcaactatt gttccagtat
     1861 tttctatgga agcacttagc aaattaagtg cttcttccca gttgtgcaat ttggaggtcc
     1921 agttggctgc caacccaagt tgttccttca ttttgtttgc aatgaagacg gggtcatcat
     1981 ttaagctgac agattttaca aatgcaagtt tctcaaaatc ttcttcgatc agatattcgc
     2041 gcaaccaaga ctggcgcctt tgcatggttt gaatagtttc aataagttct gggctcgggc
     2101 tttttggaac gccattcctg agcgacctaa aataagggat tggtataatg tcgtttggag
     2161 gctcatcaag aaagaaaaaa ccgaaaggtg ttgatgtctt attggcaaac gattccagtt
     2221 gctttacggt gggttgttca ctgccttcaa accattgctg gattttgggg aagtcttttt
     2281 tcaactcttc ccttgatttt ccagagcgat ttaaggccca agataaaata ttttgtttta
     2341 caagcacctt gctcattaag tggcctcctt tccatattga ttctaatcaa aattatttcg
     2401 aaatgaaaga agagattagt tcttttatgg gcctatgcta gaggtttatg aattgagaaa
     2461 gatgatgatt tttcaaacta tagctgttta tacagttttt acccggtctt atcagttcga
     2521 ttatttcctg tttttctttt tccgagaggt tcattttcta gttctccatg tttccaaaga
     2581 ttttgttggc ttcctcaatc ttttttagtg tctccctgaa tttttcccat tcagcctgcc
     2641 tgatgaagag tgacctgtag gattccttgc ctgcagattt gtttgcatcc ttgcaccatt
     2701 gctcaagcct tgcccttttc ctttttgcgt cttcgtcaac aatgcccttt gtttcagcga
     2761 tgtagacaaa cccgtctttt gtttttgcaa caaagtccgg ataatagttc tggattttgc
     2821 cggattctgt ggcatagtcc agtctgaaac ggctccgtct ttcatatatt ctggcaaagg
     2881 aaaccacgtc ctggcacctg tccaggaaag cggcaaattc aagctcaagc tggtttccga
     2941 cgaccatgtt ctgcaggcat tttttgctcc tgacatacct ctggcttgag acggcaattg
     3001 gctcaaaatc aagctgggag tgccctccat ggtcgactct ggggttttcc acctgcttga
     3061 gtgtcaacct ttcaatctcg gccctgaacg tgtcaaatat tgtttttgag acctccggcc
     3121 ttgtgaagtt catcagaaac accggatcat cgatttccat tatttttcca aacaacctgt
     3181 tttttgcgaa atgctccact ttgggataca gctcgttgaa acctgaaggc atccttgcct
     3241 ctttcaggat tgcagaggtg aaaaacccaa taatatttga aaagttgaca agttcaggct
     3301 tgaattcaat cttgtggtgc catgatccca aaatatcgat aaactggatt tcttttgggt
     3361 catttctcat cagcacgtcg agttttaatg gtttgaatgc aattgcatca acatcgatcg
     3421 aaaatatgca ctcagggtcc cagcgatatt tacttccaag ctttgggagg actatgtcca
     3481 ttttttccag gtccttgtcg aggtttgccc tgtccagctc gatgatgagt ggcgcgcctg
     3541 tctcatcgct tttctcgccc attgtcctgt agccaagcct gaccccttcg gatttgagcg
     3601 actcgacaaa ttcaataaag ggagggttgc caacaacaac aagctcctca acagtctgca
     3661 ggtcgaacat ttttctgaga cccctgccaa tcgcctgttc tggaaggatc tgggatttgg
     3721 ccttaaacgg cctgaggcca acgattgcag tgacatttct gacatcccag ccctcgcgaa
     3781 gcaccatcac cgagacaaca gcgaggtatg gtgaattcga actgtcaaca ctgtttgctg
     3841 catttcgcaa cacctggagt tctttttcgc tgcgtttttc atcgatttcg ccggttttgt
     3901 ttgtgtgaat aaccaatacc ctgttcttca ttacctgata atttttctct aggtgaaggg
     3961 cgatctgttc agcttctttt gtgtcttcgg ccatcacaaa caggaccggt gttttcttgc
     4021 ctttgagact atcgaaatgt tttttccatt caatgtagcc cagatgtatg aaatcgctgt
     4081 atttctcacc agcatcatca gactgcctca catccagctt gtctcttgat tctttatctg
     4141 gcagaacagg ttttttaaca atattgcatt tgatcgcctc gaccagtgga aaatctgata
     4201 cagtctgggc gaatatttcg ccgttattgt gttttggggt ggcagtcaga tcaatctggg
     4261 cagaaatacc ctttcctgta cgctggagca gcccactgtt tatatctctg attccttcga
     4321 accattgaag atttttgctg tgtatgtggt gggcttcgtc gttgaaaacc accaaatcat
     4381 ggatttgttt tgatctgatt attttgccaa gatccatccc tccactcctg tcagcgtttg
     4441 ggacaggctt ttcgccaagc cagtaagttt ctctcgttgg gagggtttca ttcatataaa
     4501 ctcgatgaat atttgtcaaa aaaatgttgc cgttttgaga aatctggcca atgtcatcct
     4561 gtatgtgtag ttcgggacaa aaatcccttt tccagtttct gccatcgtag ccattttctg
     4621 gtatcacagg gtcatttcta aaaatgcaca aattgtcaaa atctcttttt agcctttcca
     4681 gcacaattat atttggcgca acaacaagga agttttttga catcatcgag cctggctcat
     4741 aaagtttgtg gaaataagac caggcaagaa gaagcgaaag aacttttgtt tttcctgtgc
     4801 cggttgccat tttgatgcaa taacgggtcc agttttcttc aaattcctct actttgagct
     4861 ttccagatga atcaaaggca gcaaggccgt atttgtctct tatctttatg atctcataga
     4921 gataaattat tgtttcaacg gcctctcttt gagcaaaaaa gtatctcagc tcccgccctt
     4981 cagtttccaa aaaccagtga caaagcaacg attttgttgt ttgtgaggcc cccttataaa
     5041 cttggcccct ccatttgtag atttctcttc ttattttctc aacaagggga ggcagaagac
     5101 aatcatattc tttctggctc aattgttcct ggtctggaaa ccatcttttt tctattggta
     5161 tttcatcgaa aggatcgaat ttgatttcat tctctctcaa ttgctcatac cccctattgt
     5221 ttaaggtaga ggatatcatt tacttggaac aatacaacct aggccattat ctggagcaat
     5281 tagtttttgc tttgtgatag tgctgaaata taagattttt tgaataatgt taacataatt
     5341 ctaataccta tcacttatag aaattgaggt gaattaccaa aatcacttga aaaccttgac
     5401 aaaattgcgt tttttgtccc tagaagtgat aaatgttgca aaacatccca tctgtatata
     5461 caatattttg tcaatggcaa taaatggttt ataaagacat aatattggga ggtgtgaaat
     5521 gaagaaacag gaaattaaca agaaaaaaag ctttgaaaag acaatgactc caaaggcttt
     5581 tgaagcaaat agaaaaaatg ccagaaaatc cacagggcca aaaacacaga ggagtaaatt
     5641 tctaccccca tcaaacctga aacatggcat tttggcctca gtcccagttt tgccaatata
     5701 tgaaagccag aaggagtatg ataaacttgt catagaattg aagaaagatc tttgcccaca
     5761 tggcactctt gagattgtgc tggttgagaa aattgcaaat attatctgga aaaccaggag
     5821 actggcgcgt tacgaaaatg ctcatataag atgggatttt tctagggttg tcgacaatat
     5881 tttgaaagac tgggtatctt ggaaagagcg tacttccacg aatgaagatc ttgcaattga
     5941 tgtaggtaag tcaagactgg aaatattcgg tgatgaagag atggagattt ctgaaagatt
     6001 gttatctttt gttgaattcg acgacttgca agaagacgga tcccttgagg gaaagaaaat
     6061 ctttttaatc ataaatactc tttgtgattt tgaatatttg aatcggaggg aaaaacacga
     6121 gaaaatccct tgctcatcac ttgagttttt tgacgtcaaa gagttatggc cagatatttt
     6181 taatgaaaat ttctccaaaa caaaaaagca gattaaagaa attatctcga tcattgcaaa
     6241 acaatttggt aaaacacaaa aagagttata tgagagtgga cacaaacttg tgttatcact
     6301 ttttgaagaa tacaagctta tttcggaatc ggtaggtaaa gaaatggaag ttaaaatcca
     6361 acaatgcctt tttgacgaat atatttctga aaatgttgta aaacttgagg cccacttgaa
     6421 ccgccagctt ttgcaaacac tccatgaact ccagaggatc cagagcatga gagttggaat
     6481 ttctcgacca ccag
//
LOCUS       DAOSRI010000075         6468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11020_length_6468_cov_3.067363, whole genome shotgun sequence.
ACCESSION   DAOSRI010000075 DAOSRI010000000
VERSION     DAOSRI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11020_length_6468_cov_3.067363"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK717_03225"
     CDS             complement(<1..679)
                     /locus_tag="PK717_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46707.1"
                     /translation="MSFIKLAFKGLLRRPAGFIASCLTIAIASLVLILSLGLSQGYDS
                     ALLSNINSLGANILAIPKGCPYEATGVILSGGNLRYAVAENQMAPIKKAEGVKTISAT
                     IMGQRPVGPYKKVHVIVGADENYHLMRPKLGFKKPFAENEVALGYKISQVLSAKVGDD
                     FDIAGKTYKIAAIADKGATEDEDLVFMNLKSAQNLLKAEGMLSSILIQVDDPAQSDTA
                     SQRLMAIT"
     gene            complement(676..1218)
                     /locus_tag="PK717_03230"
     CDS             complement(676..1218)
                     /locus_tag="PK717_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR46708.1"
                     /translation="MRNFHKITILMLTAIILASCGSSSLPAVNPESVGMPVVSEIGEK
                     FGDATAFTWTDVGGAKKTFGPGEKQTILVFWKYGCSSCIDEIQTIQTVSDEYKVPILT
                     VCINPADTLNSVVGILGSLGLTVPVVLDPTSAVAREYSVSTIPDFMLVDQNGKITGRK
                     KGGIDIDKLLEIVDEMEGAK"
     gene            complement(1350..2633)
                     /gene="cmk"
                     /locus_tag="PK717_03235"
     CDS             complement(1350..2633)
                     /gene="cmk"
                     /locus_tag="PK717_03235"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOR46709.1"
                     /translation="MIQHPVIAIDGPAASGKTTVGALVAKKLGIKFLDSGRLYRIAAY
                     ASTKLGKGLPDILPEINLKLEDDRFIVNGEDATDKLGSVEIGELASKMSLDPRLREWV
                     NTMVRELVTEYPIIVTGRDIGTVVLPDATLKVYLDASIEERARRRHAENPGKSTYEEI
                     LEALKKRDKRDSERDIAPLVPASDAIIVDSTKMNITQVTNLIVKQARQNIKKTRITRW
                     WRFSYSVIWFLSRKLFNIEIHGRGNIPQKGPIVAIANHTSALDVLFVGLSLTRMGVYL
                     AKEEILRVPVLRTCLRAYGVVPVRRGQTSKDTVRAVSDTLSNGGLFCIYPEGTRSKDG
                     KLQGNYQTGAVQFAYKFEAKILPIGVIGAYKVMPYGSKCFKRGKVIVNVGKPIVIDKA
                     CKSSKEYYEELTEMAMKEVAQLSKQPYVPLKKSAD"
     gene            complement(2630..3565)
                     /gene="arcC"
                     /locus_tag="PK717_03240"
                     /pseudo
     CDS             complement(2630..3565)
                     /gene="arcC"
                     /locus_tag="PK717_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565769.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            3684..4565
                     /gene="pdxS"
                     /locus_tag="PK717_03245"
     CDS             3684..4565
                     /gene="pdxS"
                     /locus_tag="PK717_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOR46710.1"
                     /translation="MDKELFLVKKGLADMLKGGVIMDVVTPEHAKIAEEAGAVAVMAL
                     ERVPADIRAKGGVARMSDPQMVSEIMKAVSVPVMAKCRIGHTIEAQVLQSIGVDYIDE
                     SEVLTPADEEFHVDKWAFRVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGNVVEAV
                     RHQRAVQREIRRVVMAPDEELATIAKELGAPLELVKMVKEAGRLPVVNFAAGGVATPA
                     DAALMMHLGSDGVFVGSGIFKSGNPAKRAKAIVDAVAHWQEPEVIAEVSKDLGEAMVG
                     INIDVLDDKEKLARRGW"
     gene            4562..5134
                     /gene="pdxT"
                     /locus_tag="PK717_03250"
     CDS             4562..5134
                     /gene="pdxT"
                     /locus_tag="PK717_03250"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOR46711.1"
                     /translation="MKIGIFALQGDVAEHIKAIEASGCTPVQVRNPQDLDNLSGLVLP
                     GGESTTFWKLSNRIGLDKKIISLAKEGFPIFGTCAGLIMMSHDIEGYPNQPTWDIMDI
                     TVRRNAYGRQLESHVETIHAKDIGDVSVAFIRAPFITRVGEKVEVLSCDKEGNIIFVR
                     QGNFLGAAFHPEMTGDIKIHNFFCDIARRV"
     gene            5134..5889
                     /locus_tag="PK717_03255"
     CDS             5134..5889
                     /locus_tag="PK717_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR46712.1"
                     /translation="MSGHSKWHNIQGKKSVTDAKRGKLFTKIAKEIIVAAKRGGNPDH
                     NPSLRSALDKASEAGMPKDNIKRAIQRGTGEIEGATYEEALYEGYGPAGTAVIVEAST
                     DSKNRTISELKKIFSKHGGSLGEQGCVAWNFEREGEIIVEGGGKSLDDVLMEAADAGA
                     SDVEADGETFIVTTPPDVMHQVAETLRKAGYKVKGTNLVYNAKSTVKVTGQDAVKVLK
                     LQNDLEDHDDVNNVYSNFEMDESEMEKIASELE"
     gene            5886..6356
                     /gene="ruvC"
                     /locus_tag="PK717_03260"
     CDS             5886..6356
                     /gene="ruvC"
                     /locus_tag="PK717_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOR46713.1"
                     /translation="MKIMAVDPGLARIGWAILESDGDSFSLVECGLLQTPKEDNLPKR
                     LSEIHSKVTELISGHKVSSFAVEELFFSKNVKTAIDVAQARGVIIMSAQLMGLPVYEY
                     TPNQVKQAVTSSGSADKLQVGKMVQMLLRLPKIPSPDDVADACALGLCHLFSLR"
BASE COUNT         1627 a         1478 c         1610 g         1753 t
ORIGIN      
        1 ctgttatggc catcaacctt tgcgaagccg tgtcggattg tgccggatca tctacctgta
       61 tgaggattga agagagcata ccctctgcct tgagaagatt ttgcgccgat ttcaggttca
      121 tgaaaacaag atcttcgtct tctgttgcac ctttatcggc tatggctgca attttgtagg
      181 ttttcccggc tatgtcaaaa tcatcgccaa cttttgctga aagcacctgc gagattttgt
      241 atccaagggc aacttcattc tcagcaaatg gttttttgaa cccaagtttt ggtctcatca
      301 gatgataatt ttcgtcagcg ccaacaatca catggacttt tttgtatggc cctaccgggc
      361 gttgacccat gattgttgct gaaatggttt taacaccctc tgccttttta attggtgcca
      421 tctgattttc tgcaacagca taccgtaggt tgccaccact taaaatgact cctgtggctt
      481 cgtaggggca cccctttggg attgccagta tgtttgcgcc cagactattg atgtttgaga
      541 gaagggccga atcgtagccc tgggagagac caagagataa aatgagaact agtgatgcaa
      601 ttgcaattgt cagacaggag gcgataaaac ctgctggtct gcgaaggaga cctttaaatg
      661 cgagttttat aaaactcact tagccccctc catttcgtca acgatttcca gaagtttgtc
      721 tatatcgata ccccctttct ttctgccggt tattttacca ttttggtcaa ccagcatgaa
      781 gtccggtatt gttgatacac tatattccct tgccactgca gatgttgggt caagaaccac
      841 tggaactgtg agacccaacg aacccagtat tccaacaacg gaattcagtg tatcagctgg
      901 attgatgcat acagtcagga ttggcacctt atattcgtca ctaacagttt gtattgtctg
      961 gatttcatcg atgcatgagg agcatccgta tttccagaaa acgaggatag tttgcttttc
     1021 tccagggcca aaagtttttt tggcaccccc gacatcggtc caggtgaatg cagtggcgtc
     1081 accaaacttt tctccaattt ccgaaacaac aggcattcca acggattccg gatttactgc
     1141 cggcaaggaa gaggagccgc aagaagcaag aattattgcg gttagcatca agatcgtgat
     1201 tttgtgaaaa tttctcactt gtcacccgct ttcctttgtt ttcaaactct tatagtaaag
     1261 tttactgtta atatgtcgag tagttaatta caaatttttc gcaataacaa gatatataat
     1321 agaaaaaatt gggaaagaaa gctaaatttt caatctgccg atttcttgag tgggacatac
     1381 ggttgtttcg atagctgtgc gacttccttc atggccattt cggtaagctc ttcgtagtac
     1441 tcttttgacg atttgcatgc cttgtcaatg actatgggtt tgccaacatt tacgatcact
     1501 tttcctcgtt tgaaacactt tgaaccataa ggcattacct tgtatgcacc tataacgcct
     1561 ataggcagta tctttgcttc gaatttgtat gcgaactgga ctgcgccggt ctggtagttt
     1621 ccctggagtt tgccatcctt ggaacgagtt ccctctggat aaatgcagaa aagcccacca
     1681 tttgacagtg tatcgcttac cgccctcacc gtatcttttg aggtctgacc cctccggacc
     1741 ggaacgaccc cataagctct gagacatgtt ctgagaactg gtaccctcag tatctcttct
     1801 ttggccagat aaacgcccat cctcgtcagt gacagcccaa caaaaagtac gtccagcgct
     1861 gatgtgtgat tggcaatggc tacaatcggg cctttctgtg ggatgttgcc ccttccgtgt
     1921 atctctatgt taaaaagttt gcgtgaaagg aaccatatga cggagtatga gaacctccac
     1981 catcttgtta tcctggtttt tttaatgttt tgccttgctt gcttgacgat gagattcgtc
     2041 acctgggtga tgttcatttt tgtgctgtca acgattatgg catcactggc agggacgagt
     2101 ggtgctatat ccctttcgga gtctcttttg tccctttttt ttagtgcttc gagaatttct
     2161 tcgtaagttg acttgcctgg gttctcggcg tgccttcgtc ttgccctctc ttcaattgat
     2221 gcgtcaagat aaaccttgag ggttgcgtcg gggagcacca ccgtgccaat gtctcttcct
     2281 gtgactatta tcggatattc cgtgacaagc tcccttacca ttgtgttgac ccattcgcga
     2341 agccttgggt caagcgacat ctttgaggcc agttctccta tttcaaccga gccaagtttg
     2401 tcagtggcgt cctctccatt tacaatgaac ctgtcgtcct caagcttaag atttatttca
     2461 gggagtatgt cggggagccc cttgccaagt tttgtggatg cgtatgcggc aatcctgtag
     2521 agccttccag agtcaagaaa tttgatgccc agtttttttg caaccagtgc accaacagtt
     2581 gtttttccgg aagcggccgg cccgtcgatt gcaattaccg ggtgttgtat catctagata
     2641 ctattgtgcc ggcatcgcct tttaatgcaa gtgttatatg ggcggggtca gtgatgactg
     2701 cctgctggcc gccgttctcc aggaagtgta ttgaggcctc aatttttggt cccatagagc
     2761 cctttgcgaa atggccctcc tcggagtatt tttttgcttc ttcaagtgtc agcctgtcaa
     2821 gttggacctg gtttggtttg ccgtaattaa ggcacacttt cgggacagct gttgaaacta
     2881 gcagttttga tgcttttact tcaagggcaa gcacagcaga ggccctgtct ttatcgatta
     2941 ctgcctcgat cccggacagt ccttctggcc cttttactac cggtatccca ccgcctccac
     3001 atgctatgac aacaactcct gaatcaacga ggtctttgat ggtgtctttt tcaacgattg
     3061 attgcggcca tggcgatgca acaactcttc ttcagcccct tccggagtcc tcgacaacat
     3121 tccatccaag tgtcaaagcc ctttctttgg ccgttttttc gtccatgaag ctgcctattg
     3181 gctttgtggg gtttttgaag cccttgtccg acgaggaaac ttcaacctgg gtgaccacgg
     3241 ttatgacgtt tttcttgatc cctcttttgg acagttcttc agtgagggcc tgctggagct
     3301 ggtatcctat accgccctgt gtttctgcga cggcatagtc caacgtggtc tccgggactt
     3361 cgtgtgaggc aagctcggac cttctcataa cctgaccaac ctgcgggccg ttcccgtgcg
     3421 agactatgat accgtatccg gcactgaccg ctgctgctat atgaaacgct gtttcccata
     3481 ctgccgccct ctggttttgg acggtctggg tcatgcccgg caacagcaaa gagtttccac
     3541 caactgcaat gactattctt tccataattg acagaaagaa tatcataccg tgaattgtta
     3601 agttctcaag gtgagaagta aatacacatt tcttgaataa agcggacccg aggttaaaat
     3661 atcgttcgaa aggaaggcaa ttgatggaca aggaattatt tttggtgaag aagggcttgg
     3721 ctgacatgct aaagggtggt gtaataatgg acgttgtgac ccctgagcat gcaaaaatag
     3781 ccgaagaggc cggtgctgtg gccgttatgg cccttgagcg cgtacctgca gatatcagag
     3841 caaagggtgg cgtggccaga atgtctgatc cacaaatggt ttctgaaatc atgaaagcgg
     3901 tttctgttcc agtcatggcg aagtgcagga ttggccacac catagaggcc caggttttgc
     3961 agtccattgg tgttgattac atagacgaga gtgaagttct aacgccggct gacgaggagt
     4021 ttcatgtcga caagtgggca ttcagggtgc catttgtttg tggctgccgc aatcttggtg
     4081 aggcccttcg caggattggt gagggtgccg ccatgataag gacaaagggt gaagctggaa
     4141 ctggcaatgt tgttgaagcc gttcgccacc agagggctgt ccagagggaa atcagaagag
     4201 tcgtcatggc ccccgatgaa gaacttgcca caatagcaaa agagcttggc gctccactcg
     4261 aactggtaaa gatggtcaag gaagctggga gattgccggt tgttaacttt gctgcaggcg
     4321 gtgttgcaac tcctgccgat gcagcgttga tgatgcacct tggctctgac ggagtttttg
     4381 tcggttctgg catcttcaaa tcaggaaatc cagctaagag ggcaaaggcc attgttgatg
     4441 cggttgccca ctggcaggag ccggaagtca tcgcagaagt gtcaaaggac ctcggcgaag
     4501 ccatggtcgg aatcaatatt gacgttttgg acgataaaga aaaacttgcc aggaggggct
     4561 ggtgaagata ggcatcttcg ccctccaggg tgatgttgcc gaacacataa aggcaataga
     4621 agcttctggt tgcacacctg ttcaggtgag gaacccacag gacctggaca atctgtcagg
     4681 gcttgttctc cctggtggcg aatcgacaac cttctggaag ctctcaaaca ggatcggact
     4741 cgacaaaaaa ataatctctc ttgcaaaaga gggatttcca atatttggga catgtgctgg
     4801 gcttataatg atgtcgcacg acattgaagg ctacccaaat cagccaacat gggatataat
     4861 ggacataaca gtcaggcgca atgcgtatgg gcgccagctt gaatcgcacg tcgaaacgat
     4921 acacgccaag gacattggtg atgtgtcggt cgcgtttatt agggcccctt tcatcacaag
     4981 ggtcggtgaa aaagtggaag tgctctcctg cgacaaggaa ggcaatatca ttttcgtgag
     5041 gcaaggcaat tttcttggtg ccgcctttca cccagagatg acaggtgata taaaaatcca
     5101 taattttttt tgcgatatcg caaggagggt ttgatgtcag gacacagcaa atggcataat
     5161 attcaaggta agaaatcagt gaccgatgcc aagagaggca agcttttcac caaaattgcc
     5221 aaggaaatca ttgttgcagc aaaaaggggt ggaaatcccg atcacaaccc aagcttgagg
     5281 tctgccctgg acaaggcgtc tgaagctgga atgccgaaag acaacataaa gagggccatt
     5341 cagcgtggga ccggagaaat tgaaggggca acctacgaag aagcacttta tgaaggatac
     5401 gggccggcag gcactgccgt tatcgttgag gcctctacag acagcaaaaa ccgcacaatc
     5461 tccgaactga aaaaaatctt cagcaagcat ggcggatcac tcggcgagca gggttgtgtt
     5521 gcatggaact tcgaacgcga gggtgagatc attgtagaag gtggtggaaa gtcactcgat
     5581 gacgtcctca tggaagcggc cgatgctggt gcaagtgatg ttgaagcaga tggcgagact
     5641 ttcattgtaa caactccacc cgatgtgatg caccaggtgg ccgagaccct cagaaaggcc
     5701 ggctacaagg tcaagggaac caatcttgtc tacaatgcca agagcactgt aaaggtcacg
     5761 gggcaggacg cagtcaaagt cctcaaactc cagaacgacc ttgaggacca tgatgatgtc
     5821 aacaacgtct attccaactt tgagatggac gagtcagaga tggagaagat agcaagcgag
     5881 cttgaatgaa aataatggcc gttgacccag ggctggcccg cattggatgg gccatcctgg
     5941 agtcggatgg cgattcgttt tcgcttgtcg aatgtggatt gttgcagacc ccaaaggaag
     6001 acaatcttcc aaagaggctc tccgaaatac attcaaaagt aaccgaactc atttcaggac
     6061 acaaagtaag ctcttttgca gtagaagaat tatttttttc aaaaaacgtc aagacagcaa
     6121 tcgatgtagc acaggcaagg ggtgtcataa taatgtcggc ccagttgatg ggtctgcctg
     6181 tctacgaata cacaccaaat caagtcaagc aggccgtaac ttcatcaggt agcgcagaca
     6241 aactccaggt tgggaaaatg gtgcaaatgc ttttgaggct tcccaaaata ccctcgccag
     6301 atgatgttgc agatgcctgt gcgcttggac tttgccatct cttttcgctg aggtaaaaat
     6361 gataaatcat ttggatggaa cagtagaaga ctttgatgac gaaattgttg ccctgaatgt
     6421 gaatggcatt ggatttgagg tgcttatcgg gagaatatca gggattgc
//
LOCUS       DAOSRI010000076         6379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11207_length_6379_cov_4.331594, whole genome shotgun sequence.
ACCESSION   DAOSRI010000076 DAOSRI010000000
VERSION     DAOSRI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_6379_cov_4.331594"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="PK717_03265"
     CDS             complement(<1..1791)
                     /locus_tag="PK717_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR46714.1"
                     /translation="MNERVLGERFKLKSKLPMQDDIYLALDMESGKDVLIRTILLSSK
                     KAPRWVQFENELATIKQADSPGIVKIIEWGECDGGKYIAYELIEGEKLSQVRLNRDKL
                     IKVLINIANAIDCLHSNDIILTDLKPDNIYIVDEDYNIKIFDFIKLSSDQTSLKQMKT
                     TDVTNEVAWMVPEILRYEERTIGSNLYSFGSIGYYLCTGRPPFNDTKISSLIWQIQNL
                     QPTPVSIYNPKIPISLENLVRRLLRKNPKSRPSCMKDVLKVLEQGQKDQSLGKNVISN
                     SGSIINRGLLVGRSKELDSIKSAVLSEEVGLKTLIIDGGHGVGKTRIMQEIASLARSQ
                     DMPVLYATCEKINYKKPFGILGQLVCEAALELGFGSFGKSPYKNIFATLCPNIADRLM
                     VGNSPDLEKDPVSFNLSIPKAITWLFNECSQKGRLVVEIDDAQWCDQNSLEIINQSAK
                     EIQNSNMTVVIANSSSAKTTSQNLIGEISFSGYTQIIRLNPFSPSETAELIDIVIGLE
                     NISKETTDLLVGASKGNATYLLQMLSGMACRGYITSSGETKKISPDELPTNSNELASW
                     RIGYLDSKLKAILCEAAVIGETFDEQMLLSV"
     gene            1876..2664
                     /locus_tag="PK717_03270"
     CDS             1876..2664
                     /locus_tag="PK717_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOR46715.1"
                     /translation="MNIVWAGLSNTGKVKENNEDHYAIVPPQYPELPYIFALSDGVGG
                     RKGGDLASVRAIQVISDYFVRHLPKSYNSPEVTQKAFEIANSDLRLTGSSNANLSGMG
                     TTVVFAALFDDTLQITWIGDSRAYVIRKNNLEQVTVDHTLPNELFKRGSITEKQLKNH
                     PCKHHLLRALGIDETVKPEMVQIPAQNLEWLLLCTDGLYEHVSDIEIQGAIQKLNDPA
                     LITKKLIQMALSRGGTDNVTVVVVKMAGTQAKEDKTSKNRFGIF"
     gene            complement(2661..3167)
                     /locus_tag="PK717_03275"
     CDS             complement(2661..3167)
                     /locus_tag="PK717_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46716.1"
                     /translation="MLFVKRTLFVVLIIAGITLAALSILFFIASSANPSRVMTALIMA
                     AAAIAAFVVSSVLKRNVDQNTPAFIDADVLRLASKYDGKLTAEMVVSNLYVNKGQALE
                     SLDRLCSAGNCRAEMANDGQYYIFDSVKPKKMIRKCIYCGREQPLSQPITKCPYCGGE
                     VRVVPKDS"
     gene            complement(3169..4329)
                     /locus_tag="PK717_03280"
     CDS             complement(3169..4329)
                     /locus_tag="PK717_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46717.1"
                     /translation="MKLDLSDTIKLVDSWTGDIKQLYTELEEAQQSFNAELVKLHQDS
                     KSRLEKSAAFIKGKMDSLPGDLKNTIEKEKAAQEKLFKEKLEKIESGLAKLNKEASEI
                     EEKNTQKLVGLSKENPELNEQEEALKPKIEEAKKETLLLSRQLTKYDGISGWFAGPKV
                     RMLEKEYKKSLDRLKQLTAEIENVRKTWKKDLTDKELACNEITQRWMAIQKEVASLLM
                     EKSEIQGNFDSLVLMAALGPAIESFSGKPGLPKELDENFIAITKNKEKANLLVEGLKK
                     MSSILGSLNGISEGLSNIRNTFKGLLDEQNMHQALKKLEITVPDSATKFHSAWKDVAL
                     KVKDEKKLCENPKDLAESVDGIIKNNLTESNIKGMFEDVAGSIKEATASWKG"
     gene            complement(4319..5746)
                     /locus_tag="PK717_03285"
     CDS             complement(4319..5746)
                     /locus_tag="PK717_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR46718.1"
                     /translation="MPQCLRIQLLMENYGQFVNQVVDSTRDYVFVRDEDGVLIIKPNK
                     VHYLNRTGVELLGKMYSLPQGQSYLAVKDVANNYGVKEEDVAKDARRLLETILELLKS
                     PECMPVQVKTTSFGSHKRELPVLSEIALTYRCQNKCVFCYASSPERGKALPGMTTEQV
                     KKVLLTIKNDAKVPTVSLTGGEPTLRKDIVEIVSYAKSIGLRVNLITNGIMCGVTDLP
                     YRLKEAGLDSAQVSLESSIPEIHDRVVGHPMAFKKTVDGFKKLKSLGIHTHINTTICK
                     DNISYIDQLPKFAKEELGLEYQSMNMVIRTGHAQDHPDEVGYTEIARVLPKIKSECKK
                     AGIKLVWYSPMPYCIINTVAEGIGGTSCAASDGLLSVAPDGSILPCSSFENGVGNLLK
                     EDFRDIWFNRQSLYFRKKEFVPPVCNDCDKKNICCGACPLYWDNRGCFDELGRGRKII
                     PELIWKAKRKYVGKSRPVGGVADEA"
     gene            complement(5716..>6379)
                     /locus_tag="PK717_03290"
     CDS             complement(5716..>6379)
                     /locus_tag="PK717_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46719.1"
                     /translation="GKTPIQLELTNPKLATEPYEKVLLECVTPEGTIPRDKVSTIIDA
                     VAKSLSGKIWDADPKATRKKYQDEIENSWNLYRRGAAPVDELYTRRMIPWLILSDRYH
                     DNVRQLPNQYQPIFVGWADKAASSVENFASSLASSVEGAVSKTTEAVENALGLGIPNV
                     ANKAYDACHSACHSACHSACVHDACHSACVHSACHSACHSACVCHSACHSACHSACVS
                     SF"
BASE COUNT         1486 a         1448 c         1427 g         2018 t
ORIGIN      
        1 tactgagagc aacatttgtt catcaaatgt ttcacctatt accgcagctt cgcagagtat
       61 cgcttttaat ttcgaatcaa gatagccgat tctccatgag gccaattcat tgctatttgt
      121 gggaagctca tccggggata ttttttttgt ttcgccagag cttgtgatat agcccctgca
      181 tgccatgccg gagagcatct ggagcaggta tgtggcattg cctttcgagg caccaacgag
      241 taaatccgta gtttctttcg aaatgttttc caagccaatg actatatcga taagctctgc
      301 tgtttcggaa ggagaaaatg gatttagccg aattatttgt gtgtatccgg aaaatgaaat
      361 ttctccaatc aaattttggg aggttgtttt ggcgctggaa gagttggcta tgaccacagt
      421 catgtttgaa ttttgtattt cttttgccga ctggtttatg atttccaggc tgttttgatc
      481 gcaccactgg gcatcatcaa tttcaacaac aagtcttcct ttttggctac attcattaaa
      541 aagccacgtt attgcttttg gaatcgataa gttgaacgag acagggtctt tttccagatc
      601 cgggctgttt cctaccatta gcctgtctgc aatatttggg cacaaggttg caaaaatatt
      661 tttatacggt gattttccaa aagacccaaa tccaagctca agggctgctt cacataccaa
      721 ttgacccaat attccaaaag gttttttgta gttgattttt tcgcaggtgg cataaaggac
      781 tggcatatct tgtgaccttg caagacttgc tatctcctgc attatcctgg tcttgccaac
      841 gccatggccg ccatcgatga ttagtgtttt aaggccaacc tcttcagaga gtacggctga
      901 ttttatcgaa tcaagttctt tgcttcttcc tacaaggaga ccgcgattga ttatcgagcc
      961 tgagttgcta atgacatttt tgcccagact ctggtctttt tgtccctgct cgagcacctt
     1021 tagtacatct ttcatgcaag atggtcgtga tttggggttt ttcctcaaaa gtctcctgac
     1081 gagattttcg agcgagatgg ggattttggg gttgtatatt gaaactggag ttggctggag
     1141 gttctgtatc tgccagatga ggcttgatat ctttgtatca ttgaaaggtg gccttcctgt
     1201 gcaaaggtag tatcctattg agccaaagct gtagaggttt gaacctatgg ttcgctcttc
     1261 gtagcgcaat atctctggaa ccatccatgc aacttcgttt gttacgtctg tggttttcat
     1321 ctgtttgaga ctggtctggt cagaagaaag ttttataaag tcaaaaatct ttatgttgta
     1381 atcttcatca actatgtaga tattgtctgg tttgagatcc gtgagaataa tgtcatttga
     1441 atggagacaa tcaattgcat tggcaatatt tatcagaacc tttatcaatt tgtctcgatt
     1501 caatcttacc tgagaaagtt tctcgccctc tatgagctca taagctatgt acttgccgcc
     1561 atcacattcg ccccactcga tgatttttac aatacctggg ctgtcggcct gttttattgt
     1621 tgcaagctcg ttttcaaatt gcacccatct cggcgctttt tttgatgaaa gcaaaattgt
     1681 tcttatcaat acatctttgc cactttccat gtcaagcgcg agatagatat catcttgcat
     1741 gggaagcttc gacttcagct taaacctttc ccctaagacc ctctcgttca taattgccat
     1801 ttattttata tattattgtc ctttgcgcaa ttatgtctct tgaatttatt aggaacgcat
     1861 attaggctag aagtggtgaa tatcgtttgg gctggccttt ctaataccgg taaagtaaaa
     1921 gaaaacaacg aggaccatta tgccattgtc ccccctcagt atcctgagct tccttatatt
     1981 tttgccctat ccgatggtgt aggtggaaga aagggcggtg accttgcctc ggtgagagcg
     2041 attcaggtca taagcgacta ttttgttcgt catttgccaa aatcttataa ttcccccgaa
     2101 gtaacgcaaa aagcttttga gattgcaaat agcgacctca ggttaacagg ctcatcaaat
     2161 gccaatctga gtggaatggg aacaacggtt gtttttgctg ccctttttga tgataccctc
     2221 caaataacat ggatagggga ttcgagggcc tacgttatta ggaaaaataa tcttgagcag
     2281 gttacagttg accacactct gccgaatgag ctgttcaaga ggggctcaat aactgaaaaa
     2341 cagcttaaaa atcacccttg caaacatcat ttgttgcgtg cactgggaat tgatgaaaca
     2401 gtaaaaccag aaatggtgca aatcccggct caaaatcttg aatggctgtt gttgtgcact
     2461 gacggacttt atgaacatgt ttctgatata gaaatccagg gagccatcca gaaactaaat
     2521 gacccagctt tgatcaccaa aaagctcatt cagatggccc tttcaagagg tggaaccgat
     2581 aatgtgactg tagtagtggt aaagatggct ggtacacaag caaaagaaga caaaaccagc
     2641 aaaaacaggt ttggtatctt ctaggaatct tttggaacga ccctgacttc cccaccacaa
     2701 tatgggcatt tcgttattgg ttgtgagagt ggttgttccc tcccgcaata aatgcacttt
     2761 cttatcatct tctttggttt tacggaatca aaaatgtaat attggccgtc gtttgccatt
     2821 tctgccctgc agttgccggc agaacagagt ctgtcaagtg attcaagcgc ctggcctttg
     2881 tttacataaa ggtttgagac gaccatttca gccgtgagct ttccgtcata ttttgatgca
     2941 agcctcaaca catcagcatc aatgaacgca ggtgtattct gatcaacatt tctttttaga
     3001 acagatgaaa caacaaatgc tgctattgct gcggcggcca taataagtgc tgtcatgaca
     3061 cgcgacgggt tggcagatga tgcgataaaa aagaggatcg aaagggcggc aagggtaatg
     3121 cctgcaatta tcaaaacaac aaaaagagtt ctttttacaa agagcatctt atcctttcca
     3181 tgaagccgtt gcttctttta ttgaacctgc gacatcttca aacatcccct tgatgtttga
     3241 ttcggtgagg ttgtttttga tgattccgtc cactgattcg gccaaatctt tggggttctc
     3301 acagagtttt ttttcatcct tgaccttcaa tgctacatct ttccaggcag aatggaactt
     3361 ggtagcgcta tctgggacag tgatctccag ttttttcaac gcctggtgca tgttttgctc
     3421 gtccagcaac cccttgaatg tgtttctgat gtttgacagg ccctctgata ttccatttag
     3481 agaacccagt atcgaggaca tttttttaag accctcaacg agcagattgg ctttttcctt
     3541 gtttttggtg attgctataa aattttcatc cagctctttt ggaagacctg gttttccgga
     3601 gaacgattca attgctggcc caagcgctgc catgagcacc agcgagtcaa aatttccctg
     3661 tatctcgctt ttttccataa gcagactggc cacttctttt tgaattgcca tccatctctg
     3721 ggttatctcg ttgcatgcaa gctctttgtc tgtgaggtct tttttccatg ttttcctgac
     3781 attttctatc tccgcagtga gttgtttcag tctgtcgagt gattttttgt actccttttc
     3841 gagcatcctt acttttggac cagcgaacca tccgcttatc ccgtcatatt ttgtcagctg
     3901 tctggaaagc aggagcgttt cttttttggc ctcctctatt tttggcttga gtgcctcttc
     3961 ttgttcgttt aattcggggt tttcttttga aagccctacc agtttctggg tgtttttttc
     4021 ctcaatctca ctggcttcct tgttcagttt tgccagccca gattcgattt tttccagttt
     4081 ctccttaaaa agtttctcct gggcagcttt ttccttttcg atggtgtttt tgaggtcgcc
     4141 aggtaatgaa tccatcttgc cttttatgaa agcggctgac ttctccagcc tgctttttga
     4201 gtcctggtgc agttttacca gctccgcatt gaaagactgc tgtgcctctt caagctccgt
     4261 gtaaagctgt tttatgtcgc cagtccatga atcgacaagt tttattgtgt ctgaaaggtc
     4321 aagcttcatc agctacgcct cctaccggcc ttgatttgcc gacgtatttt ctttttgctt
     4381 tccagatcag ctctggaatg atttttcttc cccttccaag ctcgtcaaaa catcccctgt
     4441 tgtcccagta aagagggcat gccccgcaac agatgttttt cttgtcgcaa tcattgcata
     4501 ccggtgggac aaactccttt ttcctgaaat agagggactg cctgttgaac cagatgtccc
     4561 tgaaatcctc cttgaggagg tttccaaccc cgttttcaaa agaagaacag ggcagaattg
     4621 aaccatctgg tgccactgaa agcaggccat cggatgccgc acagcttgtt ccaccaatac
     4681 cttcggcgac agtgtttatg atgcagtatg gcattgggga ataccaaacc agctttatcc
     4741 ctgctttttt gcactctgat tttatctttg ggagcaccct ggcaatctcg gtgtatccga
     4801 cctcgtcagg atggtcctgc gcatggccgg tccttatcac catgttcatc gactggtact
     4861 cgagcccaag ctcctctttt gcgaattttg gcagctggtc aatatagctg atgttgtcct
     4921 tgcatatcgt tgtgtttatg tgggtgtgta ttcccagcga cttgagtttt ttgaatccat
     4981 caacggtttt tttgaatgcc attgggtggc cgaccacccg gtcatggatt tctggtatgg
     5041 atgattcgag ggaaacctgt gccgaatcaa gcccggcttc tttcaatctg tatggcaggt
     5101 ctgtgacccc acacattata ccgttggtta tcaggtttac cctcaagcca atgctttttg
     5161 cataagaaac tatctcgacg atgtcttttc gcagggttgg ttctccgccg gttagcgaga
     5221 ccgttggcac cttggcgtca tttttgattg tcaggaggac cttttttacc tgttcagtcg
     5281 tcattcctgg caatgctttt ccccgctcgg gcgaggatgc gtagcaaaaa acgcatttgt
     5341 tctggcaacg ataggtcagt gcaatctccg agagaacagg cagttccctc ttgtgggagc
     5401 caaagctggt tgttttgacc tgcacaggca tacattctgg gcttttcagc aactcaagga
     5461 ttgtttccag gagtctcctg gcgtcctttg caacgtcctc ttccttgacg ccataattgt
     5521 tggcgacatc ttttactgca aggtaggact gcccctgggg cagggaatac atcttcccca
     5581 aaagctcgac accggtcctg ttgaggtaat ggaccttgtt gggttttatg atcaggaccc
     5641 cgtcctcgtc ccttacaaat acataatctc ttgtgctgtc aactacctgg ttgacaaatt
     5701 gcccataatt ttccattaga agctggatac gcaggcactg tggcatgccg agtggcaggc
     5761 actatggcaa acgcaggcac tgtggcacgc cgaatggcac gcggaatgga cgcaagcgga
     5821 atgacaggcg tcatgtacac aggcactgtg gcacgccgag tggcaagcgg aatgacatgc
     5881 gtcataggct ttgtttgcga catttggtat gcccaacccc agtgcgttct ccacagcttc
     5941 tgtagttttt gaaactgcgc cttcaacgga tgaagcaagg cttgatgcaa agttttcaac
     6001 agaggaagcc gctttgtctg cccatccgac aaaaatgggc tgatactggt tcgggagctg
     6061 cctcacattg tcatggtacc tgtcagagag aatgagccat ggaatcatcc tgcgggtgta
     6121 gagctcgtca acaggagcgg cgccccttct gtagaggttc catgagtttt ctatctcgtc
     6181 ctggtatttt ttccttgtgg ccttggggtc ggcgtcccat attttgccgg aaagtgattt
     6241 ggccacagca tcaatgatag tggagacctt gtctcttggg attgttccct ctggggtcac
     6301 gcattcaagc agaacctttt catagggttc cgttgccagt tttgggttgg tcagctcaag
     6361 ttggattggc gtcttgcca
//
LOCUS       DAOSRI010000077         6354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11266_length_6354_cov_3.566757, whole genome shotgun sequence.
ACCESSION   DAOSRI010000077 DAOSRI010000000
VERSION     DAOSRI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11266_length_6354_cov_3.566757"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK717_03295"
     CDS             <1..409
                     /locus_tag="PK717_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR46720.1"
                     /translation="AARLVGAEDFILKLPEGYDYVLTERGATLSVGQRQLLSFARAIA
                     HDPRILVLDEATSSIDTASETLIQEAMKNMMAGRTSIVVAHRLSTIKDADLVLVISDG
                     KIAESGSHDELLKRGGMYKTLYELQFKAQERNS"
     gene            complement(753..1064)
                     /locus_tag="PK717_03300"
     CDS             complement(753..1064)
                     /locus_tag="PK717_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46721.1"
                     /translation="MNKIYLGLLCGLVFGLLDIIVMLPMKYPDARRKAEAISSAFIDR
                     FAIGFIIPNLELGINPILTGFLVGLVLSLPSAIITRVYAPIIGTGAVGGAVIGFVVTL
                     I"
     gene            complement(1296..2195)
                     /locus_tag="PK717_03305"
     CDS             complement(1296..2195)
                     /locus_tag="PK717_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR46722.1"
                     /translation="MEDLKQKLMKKHLLAPGHTACAGCGEALGMRLIIDAIGPNCIVA
                     NATGCSEIFTSKYPQSAWEVPWVHSLFENSSAIASGVEVALKARGKTGIKVVAMGGDG
                     ATADIGIGCISGMWERGHNILYVCLDNEAYMNTGIQRSSQTPFNASTTTSPSGSESMG
                     NQTLKKDMIAIANAHRVKYVATVSLANPKDLERKVKKAVETVGPSYIHFHAPCPLGWG
                     FDSDKAVEIAKLAVNTGLFPVIEYEDGQLVKVQKIAKKVPVEDYLKTQSRFRHLLKSD
                     EGKKVIAQIQVIADANIEKFGLV"
     gene            complement(2195..3367)
                     /locus_tag="PK717_03310"
     CDS             complement(2195..3367)
                     /locus_tag="PK717_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR46723.1"
                     /translation="MAIELIEGSLAIAHGAALCRPGVISAYPITPQTHIVEDLAKMVA
                     DGQLKTEFVNTESEFGAASVVLGALAGGSRAYTATTSQGLALMLEVLYNCAGMRLPLV
                     MCCANRSLSAPLSIWSDQQDAMLMRDSGFIMGFAEDAQESLDLHIMGYKIAEDERVQL
                     PYMVNVDGFLITHTFEPVNIPDQKLVDEYLGSYKPFQVLDPANPITMGCFTEPAYHMD
                     VRYQMHKANLESTKVIEEVAASFEKKFGRPSGGLIRSYKLDDADTVVVAMGSITGLAE
                     EAVDTLRGKGQKVGLLKIKTFRPFPTEKVKEALSGKKHVIVMDRAMSAGYGEILGSEI
                     RAALYGISNPPAIDSFIYGLGGRDIHEDEIIGMINRPNPGTHHSEWVGLRPDLLEE"
     gene            complement(3367..3633)
                     /locus_tag="PK717_03315"
     CDS             complement(3367..3633)
                     /locus_tag="PK717_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46724.1"
                     /translation="MSKMLKPGINVKPGTSKENKTGSWRNYYPKFDHAKCIACGTCET
                     YCPEGIIFGDKEHKYDADLDYCKGCGICANICPVKCIEMLLEVR"
     gene            complement(3630..4175)
                     /locus_tag="PK717_03320"
     CDS             complement(3630..4175)
                     /locus_tag="PK717_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HOR46725.1"
                     /translation="MKEIRIHGRGGQGCVTASEILAVAAFLDGQNSQAFPSFGSERKG
                     APIQAFARISDKKIRTRAQILHPDFLIIQDPTLIGVVDLLFGLKEDGLVIVNTAKKPE
                     ELGLKTKARVITVPALEIAVEEIGRPIPNTVMIGAFAGATGLIKIESVEKAVKERFPG
                     ELGEKNAKACRKAYNAAKEAK"
     gene            4259..4993
                     /locus_tag="PK717_03325"
     CDS             4259..4993
                     /locus_tag="PK717_03325"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011762545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOR46726.1"
                     /translation="MDEIKNAVDLLLKAGRVSALTGAGMSAESGIQTFRDKDGVWTKM
                     DPMKWATREGYLENPKLVWEWYEARRNQVKNSKPNDGHCAMAEFENIFGHFSVATQNI
                     DGFHKLSGSKNVHELHGNIFRNKCFDEDKMLEELDETSIPPRCPYCGALARPDVVWFG
                     EILPQEVWEAAFDDAKNSDFYMVVGTSGTVYPAAGLAGIAKKNGAKVIIINPEDSEID
                     LLADVLIKGKSGEVLPEILRGLQTSK"
     gene            5117..6046
                     /locus_tag="PK717_03330"
     CDS             5117..6046
                     /locus_tag="PK717_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOR46727.1"
                     /translation="MKRVIFAVFLISVMAILFVGPVFSQENKNSPPPTTITGVPSTPD
                     EIMAAQKRNYVQFPILNGKYGQIWLIPKGEAKITARNIKGLIAGDVIEVDNDGTSACS
                     IDFDNFTSVYLKSDSAIEFVDTTPTKMKIKLLKGDMLFASKKSSGIELVQDGWATQGL
                     EGTFTFSVTRDSSEMSVVEGTTAFKIEKSGSVYKIDAGNTAIITKDGIGEIDPAGISP
                     LRFWDDTISNLNPTLPKSKEAVEPSTAFSLGAILLIVVAAIIVGLLVYVVYRTINKRP
                     KVEIEKVDEPKPRTEDIVASEIEEITTDETIIK"
     gene            6095..>6354
                     /locus_tag="PK717_03335"
     CDS             6095..>6354
                     /locus_tag="PK717_03335"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOR46728.1"
                     /translation="MKTLSDGKEWNKKDIFESVAKILNLSEEEKNERIPSGPLKYQKR
                     MELSFFYLVKAGLIERTKLANLLITEEGRKLLKENPPIIDNN"
BASE COUNT         1647 a         1412 c         1579 g         1716 t
ORIGIN      
        1 agcagccagg cttgtcggcg ccgaagattt catccttaaa ctgcctgaag ggtatgacta
       61 tgttctgacc gaaagagggg caacactctc tgttggtcag cgtcagctcc tttcattcgc
      121 cagggcaatt gcccatgacc caagaatact tgtcctagat gaggcgacaa gctctatcga
      181 cacggcctct gaaacgctga tccaggaggc catgaaaaac atgatggctg gcaggacctc
      241 gattgttgtg gcccacagac tctcaaccat caaggatgcc gatcttgtgc tggttatctc
      301 tgatggaaag attgcagaga gcggatcaca cgatgagctt ttgaaaaggg gtggaatgta
      361 caagaccctt tatgagctcc agttcaaggc ccaggaaaga aactcataac aaagtaaaac
      421 agcccctctc ttggaggggc tgttggaaaa cgaatgccaa attttttctt cagagagtaa
      481 acaaaactat tcagatcgtt ccctttcttg gtaaatggaa agccactcga atccttctct
      541 ctccaagcag atgagaaatt actcaaatcc ttccccctcc tcagagcaag taggaaatca
      601 ctcagatcct tctcccgaag ggagaaggaa aaaggatgag ggggttttaa aaaccttcaa
      661 aaccacccaa aaaccacctc acccgaaccc tctcctggaa ggagaggggc aaaaatggga
      721 gcctttttcc tgaaaaaagg gtttgtattt aatcagatga gcgtgaccac aaaacctata
      781 actgccccgc caactgcacc tgttccgatg attggcgcat agacccttgt gattattgca
      841 gatggaaggc tgagcacgag gcccaccaga aaacctgtca ggattgggtt gatgcccaat
      901 tccaggtttg ggatgataaa tccgattgca aagcggtcaa tgaaagctga agagatggcc
      961 tctgctttgc ggcgcgcatc tggatatttc attggcagca tgacgatgat gtccaggagg
     1021 ccaaaaacca gtccgcataa taatcccaag tatattttgt tcatgttttg attatggctg
     1081 gcttgttttc aaaatacaac aatctggatt ttttgtgcaa gtttgttttg ggaacttcca
     1141 atgccctggt agtacaaatt tgagtaatag ttaagagaga accttggata aatttctaag
     1201 attattccaa atcctcagca tttttgagat tgatttttca aataaaaaag ccggaggcga
     1261 aacctccggc tttttggttc tctgaagaga ggctatcaga caagcccgaa tttttcgatg
     1321 tttgcgtcgg caattacctg aatttgtgcg atgacctttt tgccttcgtc tgatttgagc
     1381 aggtgcctga aacggctctg ggtcttgagg tagtcttcca ctgggacttt tttggcgatt
     1441 ttctggactt ttacaagctg cccgtcttcg tattcgatga cagggaaaag acctgtattt
     1501 acagcaagtt ttgcgatctc gactgctttg tccgaatcaa aaccccaacc aagtgggcat
     1561 ggggcgtgga aatggatgta ggacgggccg actgtttcga cagccttttt caccttgcgc
     1621 tcaaggtcct tcgggttggc aaggctcact gtggctacat atttgaccct gtgtgcgttg
     1681 gcaatagcga tcatgtcttt tttgagggtt tggttgccca tggactccga gccggatggg
     1741 gaggtggttg tgcttgcatt gaatggggtt tgcgaggagc gctggatgcc tgtgttcatg
     1801 tatgcctcgt tgtccaggca gacgtaaaga atattgtgtc ccctctccca cattccggag
     1861 atgcagccta ttccaatgtc ggctgttgcg ccatcgccgc ccattgccac gaccttgatg
     1921 ccggttttgc ctcttgcttt gagggctacc tctacaccag atgcgattgc ggagctgttc
     1981 tcaaagagcg aatgcaccca cggcacttcc caggcggact gtggatattt tgaggtgaaa
     2041 atctcggaac aacccgttgc gttggcaact atacagtttg ggccgattgc atcgataatc
     2101 agcctcatgc cgagtgcttc accgcaacct gcacaggcgg tgtggccagg ggccagaagg
     2161 tgctttttca tcagtttttg cttaaggtct tccattattc ctccagaagg tccggcctga
     2221 gcccgaccca ttcggaatgg tgggtcccgg ggtttggcct gttgatcatt ccgattatct
     2281 cgtcttcatg gatgtccctg ccaccgaggc cgtagataaa gctgtcaatt gctggcggat
     2341 tggatattcc ataaagggcg gccctgattt ctgagccaag gatttctcca taacctgcgg
     2401 acattgctct gtccatgaca atgacgtgct tctttccaga aagggcttcc ttgacttttt
     2461 cggtcgggaa tggtctgaag gtcttgatct tgaggaggcc aactttttgg cctttgcccc
     2521 tgagcgtgtc aacagcttct tcggcaagac cagtgattga gcccatggca acaacgacag
     2581 tatcggcatc atcaagcttg tagcttctta tgagaccgcc cgacggcctt ccaaatttct
     2641 tctcaaatga tgcagcaact tcctcgatta cctttgtgga ttcgaggttg gccttgtgca
     2701 tctggtatct gacgtccatg tggtatgctg gctcggtgaa gcaacccatt gttattgggt
     2761 tggccgggtc gagcacctgg aatggcttgt aggagccaag atattcatca acaagtttct
     2821 ggtccggaat gtttactggt tcgaaggtgt gggtgatgag gaaaccatca acgttgacca
     2881 tgtatggtag ctggaccctc tcgtcttcgg ctatcttgta acccatgatg tgaagatcaa
     2941 gcgattcttg tgcgtcttcg gcaaatccca tgataaaacc gctgtcgcgc atgagcattg
     3001 catcctgctg gtcggaccag atggaaagcg gtgctgaaag cgacctattt gcgcagcaca
     3061 ttacgagtgg aagtctcatt ccagcgcagt tgtaaagcac ctcgagcatg agtgccaggc
     3121 cctgggatgt tgttgcagtg taggccctgg agccgcctgc aagagcacca agaacgactg
     3181 atgcggcacc aaactcggac tcggtgttga cgaattcggt tttgagctga ccatcggcga
     3241 ccattttggc gaggtcctcg actatgtgcg tctgtggggt gattggatat gcagagatga
     3301 cacctggcct gcaaagagcg gcgccgtggg ctattgccaa gcttccttcg atgagttcaa
     3361 ttgccattat ctcacctcca ggagcatttc aatgcatttt actgggcaaa tgtttgcaca
     3421 aattccgcaa cctttgcaat aatcgagatc tgcatcgtat ttgtgttctt tgtccccaaa
     3481 aattatccct tctgggcagt atgtttcgca tgttccgcat gcgatacact ttgcatggtc
     3541 aaattttggg taatagttgc gccaagagcc ggttttgttt tcttttgatg ttccgggctt
     3601 gacattaatt cctggtttca acatcttgct catttggcct cctttgccgc attgtaggcc
     3661 tttctgcagg ccttggcgtt cttttcgcca agctcgcctg ggaacctttc cttaacggcc
     3721 ttttcgaccg attcgatttt aatgagccct gtggctccgg caaatgcacc gatcattacg
     3781 gtgttcggaa ttggtctgcc aatttcttct acggcaattt ccagtgccgg gacagttatt
     3841 actctggcct ttgtcttgag gccaagctct tccggttttt ttgccgtatt gacgatcacg
     3901 aggccatctt ccttgagtcc gaacagaaga tcgacaacac cgatcaaagt tgggtcctgg
     3961 atgatcagga aatctggatg aaggatctgg gcccttgtcc tgattttctt gtcagaaatt
     4021 ctggcgaagg cctgaatggg cgcacctttg cgctcggaac cgaagcttgg gaatgcctgg
     4081 gagttctgtc cgtccaggaa tgccgcaaca gcaaggattt ccgaagcggt gacgcaccct
     4141 tgacctccgc ggccgtgaat gcgtatctct ttcatttttt ctcacctttc caattgtatt
     4201 atgagccacg gtggattata aacacaaagg cactttatca aagggtggta tggtaaacat
     4261 ggacgaaata aaaaatgccg ttgatttgct gttaaaagct ggcagggttt cggcccttac
     4321 gggggctggg atgtctgccg agagtggaat acagactttc cgtgacaaag atggcgtctg
     4381 gacaaagatg gatccgatga aatgggcaac cagagagggc tatcttgaaa atccgaaact
     4441 agtgtgggaa tggtatgaag caagaagaaa ccaggtgaag aactcaaagc cgaatgatgg
     4501 acattgtgca atggcagaat ttgagaacat tttcggtcat ttttcggttg ctacacaaaa
     4561 tatagatgga tttcacaagc tctctggttc aaaaaacgtg catgagcttc atggcaatat
     4621 attcaggaac aagtgctttg atgaagacaa aatgcttgaa gaacttgatg aaacaagcat
     4681 tccaccaaga tgcccatatt gtggcgcttt ggccaggcct gatgtggtgt ggtttgggga
     4741 gatactaccg caagaagttt gggaggctgc ttttgatgat gcaaaaaact ctgatttcta
     4801 catggtggtt ggtacttcgg gaactgtcta tcctgccgct ggccttgccg ggattgcaaa
     4861 aaagaatggc gcaaaagtga taattataaa tcctgaagac tccgagattg atttgctggc
     4921 agatgtttta ataaagggca agtcgggaga agttctgcca gaaattctca gagggctcca
     4981 gaccagtaaa tagccaggat tacccataaa ggtttcaaag ggatttttct gctctctacc
     5041 aacttttggc aaaagattga caaagccttc ttttggaaat aacttaatta caatctgata
     5101 ttgccaggag gacgggatga aaagggttat ttttgcagtc tttttgatat cggttatggc
     5161 cattttgttt gttggccctg ttttctcgca ggaaaacaag aattcaccac caccaacaac
     5221 tattacaggc gttccatcca cgccagacga gataatggcc gcccagaaaa gaaattatgt
     5281 ccagtttcca attttgaatg gtaaatatgg ccagatatgg ctgattccca agggagaggc
     5341 aaaaataacc gccaggaaca taaaagggct cattgcaggt gacgtcatcg aggttgacaa
     5401 tgatggaaca agtgcctgtt caattgattt tgacaacttt acctcggtct atctcaaatc
     5461 ggatagtgcc atcgagtttg tcgacactac accaacaaaa atgaaaataa aacttttgaa
     5521 aggtgacatg ctctttgctt caaagaagtc ctcagggatc gagcttgtcc aggacggctg
     5581 ggcaacccag gggcttgaag gaacgttcac cttttcagtg accagagaca gctccgagat
     5641 gagcgttgtt gaaggcacga ctgcattcaa aatagaaaaa tcggggtccg tttacaaaat
     5701 agacgcaggc aacactgcaa taataacaaa ggatggcatt ggcgagattg acccggctgg
     5761 catatcgccg ttgcgtttct gggatgacac catatcaaac ctgaacccga cactgccaaa
     5821 atcaaaagaa gctgttgagc catcgaccgc tttttcgctt ggcgcaattt tgctgattgt
     5881 tgttgcggca atcattgttg gtttgcttgt ttacgttgta tacaggacaa tcaacaagag
     5941 gccaaaggtt gagattgaga aagttgatga gccgaagccg cgcacagaag atattgttgc
     6001 atccgagatc gaggagatca ccactgacga gacgataata aaataagaga ggaaattaca
     6061 tatggtgagc tgcaattatt tctatatgcc tattttgaaa accctgtctg atggcaagga
     6121 atggaacaag aaggatatat ttgaatctgt agcaaaaata ttgaatttaa gtgaagagga
     6181 aaaaaatgag agaataccat ccggcccact aaaataccaa aaaaggatgg aattatcttt
     6241 cttttattta gtaaaagctg gtcttatcga aagaactaaa cttgccaatc tccttattac
     6301 tgaggaaggt agaaaattat tgaaggaaaa ccctccaatt attgataaca attt
//
LOCUS       DAOSRI010000078         6258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11513_length_6258_cov_4.130904, whole genome shotgun sequence.
ACCESSION   DAOSRI010000078 DAOSRI010000000
VERSION     DAOSRI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11513_length_6258_cov_4.130904"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK717_03340"
     CDS             complement(<1..777)
                     /locus_tag="PK717_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR46729.1"
                     /translation="MWTIGEFENGVLSEATAEVLGCLSPVQDNQLAESNLVLVGSNLL
                     MHGSVFGRLGQPVTILIESDDLADLEPGIVAREVADLAQNRKPSIIALPCTVWGSDVA
                     ARVAARLNYPLVTDVVGLDATEDMITIERLSFGGQNKAMISLPAECVVTIHKRVFSQP
                     RPADITATLERLEAATSKADIKKVIEKVLPLSSEGPALIDSPVIISGGRGMGSPENFK
                     LIKELAKKLGAAYGASRAVVDSGWIDSSHQVGLTGKVVSPR"
     gene            complement(783..1568)
                     /locus_tag="PK717_03345"
     CDS             complement(783..1568)
                     /locus_tag="PK717_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOR46730.1"
                     /translation="MKIVVCIKQVPDPEKFSAGSFERDWRLSRDSYPKTTNPVDKNAL
                     ELALQLAGQDPVEVITVGPESAKEALREMLSIGASSAVHVKDPALEGADLLSTTIALS
                     AAIKKRGPFDVIICGKQSTDAGNASIPSMLAEMLGLPSSTGCEKVEFVDGKLFAYQPG
                     DLGMVVWKIELPCVLSVTKNINTPRLPSLRGMTKAMQTEIVMYDLQSLGIESLPEGRL
                     QPGNIISSKRITREITTQIFEGETSQIKAAALLMSLREKGLIQ"
     gene            1629..3104
                     /locus_tag="PK717_03350"
     CDS             1629..3104
                     /locus_tag="PK717_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46731.1"
                     /translation="MSKEPKRFHLATALIIAVLSINATIANALPDGSIAINIGKFANT
                     FQAPLNSKSTKCTYCIHPDLDFINPQSQIFIVQVRTMEKDPPLCTLKLSADGGNITPT
                     TANTGKGNFRTAFDKNDVPFGKSIIKAQAIDDSGQIIGEADLYVKRTRYYISVDKIIQ
                     ECDTCNDITTIPLIVDFENTIPASQVYASIIATNNTESDIFFTASSSQPWVVIQNGPQ
                     FSLQAGGSVAIKFGINWNFAKPNQVVQSRITLYGKGIKHEFPIGATILADVPVCAKSL
                     PQNIDFGYTPRGTSFTKKLILLGSPKFEMVCNDGWLKFDPKSTEEGREILVTINASLL
                     PYGKSHNTKLVALPKGACQLVFVPISVSTDPSISVSLVTGSKQATINSKAFQLDSPAK
                     IISSRVMVPLRFISDAFGASIVWNQKTKMIVITRLSKTITMKLGENTATVDGKQVNLD
                     VAPTVIDGRTLVPIRAISDFFGATTGWNSQTKTAKIDWDPD"
     gene            3489..4775
                     /locus_tag="PK717_03355"
     CDS             3489..4775
                     /locus_tag="PK717_03355"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOR46732.1"
                     /translation="MSEKGFNAFKMAQEQFDKVADMLDLDEGTRQLLRFPQREYHIRI
                     PVRMDDGSVKVFQGYRVQHNDARGPCKGGIRFHPQETADTVRALATWMTWKCSVVDIP
                     LGGGKGGVICDPHNLSMREQEQICRGWVRQVAINIGPLQDVPAPDVMTNGQHMLWMLD
                     EYEKITGARYPGVITGKPVGMGGSKGRTEATGYGVVYTIREALKLMGIDPKNTTAAVQ
                     GFGNVAQYAVDLYTKLGGTVICVSCWSQEDQTSYTFKKSTGVSLDELRKITDRFGGID
                     KKKAQDLGYQVLAGDAWIEQEVDILIPAALENQITGENVNRISKKVKIIGEGANGPTT
                     PEADKVIHERGIFVIPDFLANAGGVTCSYFEQVQCNMNYFWEKDEVLNKLDTKMTSAF
                     HEVASLASKKKIYMRDAAYMIAVSRVAEACRLRGWV"
     gene            4912..5406
                     /locus_tag="PK717_03360"
     CDS             4912..5406
                     /locus_tag="PK717_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46733.1"
                     /translation="MNESEINIQRITVSDKNMVSRICIASPAPDPKVDGTIYKRDTAI
                     TGIQLMDCNGNELGGIGVMDNQRAAVFALDYSRHEAVGMYAFDTPEAGGACIFINGKD
                     KKAEVMGSKKYSKAELKIENDSPVLTFSGKDGRPRIIIGLDENDNPVIQILGKDGRMR
                     NLIE"
     gene            5536..6015
                     /locus_tag="PK717_03365"
     CDS             5536..6015
                     /locus_tag="PK717_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR46734.1"
                     /translation="MAEYDQNNLEWLLRVFDRMHHTYLKSALEKQGLKVTHPYILVAL
                     HKIKGMKASQKELGDAIGISPPTVAISVKRMEKAGLLCKISDEKDLRRNVITLTKKGV
                     EYIEECLSIFDKTDRSVFDGFSELEREQLKRFYLRMIKNLETMGARLPNQLKRSEQG"
     gene            6012..>6258
                     /locus_tag="PK717_03370"
     CDS             6012..>6258
                     /locus_tag="PK717_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR46735.1"
                     /translation="MIKRLYAYVGQYKKNILYCFLAVTVDVVCEMMIPLLMAKIVDNG
                     IPQKDIGFIAGMGGIMVLLAVVAIGFGIINMKFSADAS"
BASE COUNT         1868 a         1426 c         1415 g         1549 t
ORIGIN      
        1 ccttggagag acaaccttgc cggtcagacc aacctggtga gaggagtcta tccatccgga
       61 atcaacgact gctctggagg caccatatgc agcaccaagt tttttggcaa gttcttttat
      121 cagcttaaaa ttctccggag atcccatacc cctgccgccc gaaataatga ctggtgagtc
      181 aatgagcgcc ggaccttcag aagagagtgg aagtaccttc tcaataacct tcttgatatc
      241 agcttttgat gtggcggcct ccagcctttc aagtgttgct gttatatctg ctggtcttgg
      301 ttggctgaaa accctcttgt ggattgtcac aacacactct gccgggaggc tgatcattgc
      361 cttgttctgg ccaccgaatg agagtctttc aatagttatc atgtcttctg tggcatcaag
      421 tccaaccacg tcagtgacaa gaggataatt cagccttgct gctactctag ctgcaacatc
      481 agagccccaa actgtgcaag ggagtgcgat aatcgatggt tttctgttct gggccagatc
      541 agcaacttct ctggcgacaa ttcctggctc gagatcagca agatcatccg attcaatgag
      601 gatggtaacc ggttggccaa gccttccaaa aaccgaacca tgcatcagta ggttggaacc
      661 aaccagaaca agattagatt ctgcaagctg gttgtcctgc accggggaga gacaccctaa
      721 aacctctgct gtcgcttcag agagaacgcc attctcaaac tcgcctatcg tccacatgta
      781 cttcactgga tcaacccctt ttctctaagc gacatcaaaa gcgcggcagc tttaatctgc
      841 gatgtttcac cttcaaaaat ctgggtggtt atttccctgg tgattcgttt tgaacttata
      901 atattgccgg gttgcaacct gccctccggc aaactttcta ttcccaaact ttgcaggtca
      961 tacatcacta tttcagtctg catcgctttg gtcattcccc taagtgatgg gagtcttggc
     1021 gtgttgatgt tttttgtaac agataaaaca catggaagct caattttcca gaccaccatc
     1081 ccaaggtcgc ctggctggta agcaaaaagt tttccatcca cgaattcgac cttctcacat
     1141 cctgtggatg aaggcaaccc aagcatctct gcaagcatgg aaggaatgga ggcatttccg
     1201 gcatctgtgg attgcttgcc acagattatt acatcaaaag gccccctctt tttgatcgct
     1261 gcagaaagtg caatggtggt tgagagcaag tcagcccctt caagcgctgg gtctttgacg
     1321 tggactgctg atgacgcgcc aatcgaaagc atctctctca atgcttcctt ggcgctctcc
     1381 ggaccaacag taattacctc caccgggtcc tggccagcta gctggagagc aagttccagc
     1441 gcattcttgt caactggatt agtagttttt ggatatgaat ctctcgaaag tctccagtct
     1501 ctttcaaaag agcctgccga gaatttttca ggatcaggga cctgctttat gcaaactacg
     1561 attttcatga agcctccaaa aaactttgtg ttgaccacaa cgacaccgag gtgtataaaa
     1621 tgctagcggt gagcaaagaa ccgaagagat ttcatttggc cacggcgctt atcattgctg
     1681 tgttgtcaat caacgctacc atagcaaatg ctttgcctga tggcagcata gctattaata
     1741 ttggcaaatt tgccaatact tttcaagccc ccctgaattc caaatccaca aaatgcacct
     1801 actgcataca tcctgacctg gacttcataa atccgcaatc ccaaatattc atagtacaag
     1861 taagaaccat ggaaaaagac cctccactgt gcacactcaa gctatcggct gatggaggga
     1921 atattacacc taccaccgca aacacaggaa aaggaaattt taggactgct tttgataaaa
     1981 acgacgtccc ttttgggaaa tccataatca aggcccaggc aatcgatgat tctggacaaa
     2041 tcattggtga agcagatttg tacgtgaaaa gaaccagata ttacatctca gtggacaaga
     2101 tcatccagga atgcgatact tgcaacgata taacaactat tcccctcatt gttgatttcg
     2161 aaaacacaat accagcatcg caggtttatg cgagtatcat cgcaacaaat aacaccgaat
     2221 ccgatatttt ctttactgca agttcaagcc agccgtgggt agtcattcaa aatggccccc
     2281 agttctctct gcaagcaggt gggtcagttg caataaaatt cggtatcaac tggaatttcg
     2341 ccaaaccaaa ccaggtggtc cagtcaagaa taaccctgta cggcaaggga ataaaacacg
     2401 aatttcctat cggggcaacg atcctggctg atgtaccggt ttgtgcaaaa tccttaccac
     2461 aaaatatcga ttttggatac actccaaggg gaacctcttt cacaaaaaaa ctgattttgc
     2521 ttggttcccc aaagtttgag atggtatgca atgacggttg gctaaaattt gatccaaaat
     2581 ccacggaaga gggcagggag attttggtaa ccatcaatgc aagcctcctt ccatatggca
     2641 aatcacacaa tacaaaactg gttgcactcc ccaagggtgc ttgccagctc gtatttgttc
     2701 ctatttctgt cagtacagat ccaagcattt cagtgtcgtt ggttaccgga tcgaaacagg
     2761 caacaataaa ttcaaaagcc tttcagcttg attcaccagc aaaaataata agctccaggg
     2821 tcatggtccc gctcaggttc atttctgatg ctttcggggc cagtattgtt tggaatcaaa
     2881 aaacaaaaat gatagttata acaaggctct caaaaacgat tactatgaag ctcggggaga
     2941 acacggccac agtggatggc aaacaggtga atctcgatgt ggcaccaact gtgatagacg
     3001 ggaggacgct tgtcccaata agggcaatat ctgatttctt tggcgcaaca actggctgga
     3061 acagccaaac aaaaactgcc aaaattgact gggacccgga ttgattgaca cccaagcgtt
     3121 ttcaagaggt tttttctcgg ttttcatcag gcggcacttt aaaaagtgat taaaacagtt
     3181 ttcacgagtt taattgtgtc atacttccca aactcagatg cgcagtattt tgcaaatcgt
     3241 taccagtttc acaaattcaa ggattcacag cgacattcaa taatatattt ttgtcaatgc
     3301 ccataaaata tttaacataa agtactacaa ggcatgtaca atatttatgt agaaggttta
     3361 caaaagaacc atttgccaac aaaataggcc ttgactcttt gtaccattgg actaataaag
     3421 attcgggttt aaaaggtatc attgcctcca aagaaatgag ggggctaaaa taaaaaagga
     3481 gttgtaaaat gtcagagaag ggtttcaatg cattcaagat ggcccaggag cagtttgaca
     3541 aggtagccga catgcttgat ctggatgagg gcaccaggca gcttctccgc tttccgcaga
     3601 gagagtacca catccgcatt ccagtccgca tggacgatgg ttcagtcaaa gtttttcagg
     3661 gatacagggt tcagcacaat gatgctcgtg gaccatgcaa gggtggaatt cgcttccatc
     3721 cgcaggaaac tgcagacaca gtaagggcac ttgccacatg gatgacctgg aagtgttcag
     3781 ttgtagatat tcctcttggt ggcggaaaag gtggagtcat ctgcgatcca cacaatctca
     3841 gcatgaggga acaggaacag atttgtaggg gctgggttcg ccaggttgcc atcaatatcg
     3901 gaccgctcca ggacgtcccg gcaccagacg tgatgaccaa tggtcagcac atgctctgga
     3961 tgctcgatga gtacgagaag atcacaggcg ccaggtatcc gggagtcatt accggaaaac
     4021 cagttggcat gggcggatca aagggcagaa ccgaagcaac aggttatggc gttgtataca
     4081 caattaggga agccctcaag ctcatgggaa tcgacccaaa gaacacaaca gcagcagttc
     4141 agggtttcgg aaacgttgcc cagtacgctg ttgatctcta cacaaaactc ggcggaacag
     4201 tcatctgcgt ttcctgctgg agccaggaag accagacttc atacactttc aaaaagtcaa
     4261 ctggtgtaag ccttgatgaa ctcaggaaaa tcaccgacag gttcggcgga atcgacaaga
     4321 agaaagccca agacctcggg tatcaggtac ttgcaggtga tgcatggatc gaacaggaag
     4381 ttgacatact catcccagca gcacttgaaa accagatcac tggcgaaaac gtaaatagga
     4441 tttccaagaa agtcaaaatc atcggcgaag gcgccaatgg cccaaccact ccagaagcag
     4501 acaaggtcat ccacgaacgc ggcattttcg tgatcccgga cttccttgcc aacgccggtg
     4561 gagtcacctg ctcctatttc gaacaggtcc agtgcaacat gaactacttc tgggaaaagg
     4621 atgaagtcct caacaagctc gacaccaaga tgaccagcgc attccacgaa gttgcatcgc
     4681 ttgcctccaa gaagaaaatc tacatgagag acgcagccta catgatcgct gtctccagag
     4741 tcgctgaagc ctgcaggctt cgtggctggg tgtgataacc tcccccaaat aaaaatttaa
     4801 gcaatcaaaa gccgctccaa aaagagcggc ttttttattt tcctgtattc atttcatgcc
     4861 gtatgataga aatactcaaa aactttttca ggccagagag ggaaaactgc aatgaacgag
     4921 agcgaaataa acattcaaag aatcacggtg tcagataaaa atatggtatc aaggatatgc
     4981 attgcctcac cggcgccaga cccaaaagtt gacggtacca tttacaagag agatacagcc
     5041 atcacaggca tccagctgat ggactgcaac gggaacgaat tgggtggcat cggtgttatg
     5101 gataaccaga gagcggctgt gtttgcattg gactattcga ggcatgaggc ggttggcatg
     5161 tacgcttttg atacaccaga agctggtggg gcttgcatct ttatcaatgg caaggataaa
     5221 aaagcagaag taatgggatc aaaaaaatat agcaaggcag agcttaaaat tgagaatgac
     5281 tcacctgttc tcacattttc aggaaaggat ggcagaccaa ggataatcat tggactggac
     5341 gaaaacgaca accctgtcat tcagatcctg gggaaagatg gccgaatgag gaatcttatc
     5401 gagtaaactc ctcaaaattt ctccttccct tccttaaaat ttttggctga atgtttgaaa
     5461 ttgtttattg acataataaa aatgccataa tattatttgt cgacaaatta ttttatataa
     5521 aacaaaggag gtcgaatggc ggaatacgac caaaacaatc ttgaatggct gcttcgcgta
     5581 tttgaccgta tgcatcacac ttacctaaaa tcggcgctag aaaagcaagg actcaaagta
     5641 acccaccctt acatcctagt tgcattgcac aagataaagg gtatgaaagc ttcccaaaaa
     5701 gaacttggcg atgccattgg aatcagccca cccactgttg cgatctcagt caagcgcatg
     5761 gaaaaagcag ggctgttatg caaaatatca gatgaaaaag atctgcggcg caatgttatt
     5821 acactcacaa aaaagggtgt ggagtatata gaagaatgcc tcagtatatt cgacaaaaca
     5881 gacaggagcg tatttgatgg gttctcggaa ctggaacggg aacaacttaa aaggttttat
     5941 cttcgcatga tcaagaatct cgaaacaatg ggtgcgcgat tgcctaacca actaaaaagg
     6001 agtgagcaag ggtgataaaa aggctatacg cttatgtcgg gcagtataaa aagaacatct
     6061 tgtactgctt tttggcagtg acggttgatg tggtttgtga aatgatgata ccgcttttaa
     6121 tggcaaaaat cgtggacaat ggcattccgc aaaaggacat tggctttatt gccggaatgg
     6181 gtgggattat ggttttgctt gcagtggttg ctattggatt tggcataatc aacatgaaat
     6241 tttcagctga tgccagcc
//
LOCUS       DAOSRI010000079         6221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11588_length_6221_cov_3.970645, whole genome shotgun sequence.
ACCESSION   DAOSRI010000079 DAOSRI010000000
VERSION     DAOSRI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11588_length_6221_cov_3.970645"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PK717_03375"
     CDS             complement(<1..1672)
                     /locus_tag="PK717_03375"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HOR46736.1"
                     /translation="MCGIFGIVYKEDKDDMGKTLTKAAKSLTYRGYDSVGAVAVTKTG
                     KADLRKDIGKVDEVAKKLNFEELCGNRGITQLRWATFGVPAQRNAQPHFDTKGRLVGA
                     HNGNVVNTIHLIKELTAKGHVFRGENDGEVVVHVIEDSFNRHGDLDLAIREARKILKG
                     DYAYAITALKGEKMFCTKMGSSLYLGVGDGFVCCSSDLPSILNLTNQIVPLNDGEYVE
                     FDSDSYEIRNIENGEKIDRKPITSPLSPESASKGDFPFFMEKEIFEQPERAQALLDYL
                     AECDELVPFAEEIASGDRVYLVGSGSSYNACVIGSYYLNKLGGCATFPVVAGSFIEHF
                     GEGIRPTDLFVLVSQSGETKDVINVLNMLTARGMANNVLGVVNVLGSTLMMRSRRHLP
                     LLSNLEIAVAATKTFINQSVLFLALAIEVAKLKGRAGDLPETLAKTPKAIKGTIDQMN
                     GWAKELAEKYKDCVDMYTLGYGATYGSALEAAQKIKEISYAHCEGLYSAEFKHGPLAI
                     VTEGYPVFYLTTVEDQHMTLSHINEVTCRLGSAITVAPPSDLLAKNSTA"
     gene            complement(1680..2288)
                     /gene="recR"
                     /locus_tag="PK717_03380"
     CDS             complement(1680..2288)
                     /gene="recR"
                     /locus_tag="PK717_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR46737.1"
                     /translation="MNYPVKAFQELVDNLHKLPGIGPKTAERLSLFILGMDPEEARKI
                     SDAIGKLHERVKRCPVCFNLSDGGECPVCLDEKRDHSIVLVVEEAKDVLAIERAGSFS
                     GVYHVIGGTIRLASGIGPEKLNIDGLIARVRNGGIKEIILATNLTHEGNLTAMYIKKE
                     LDGIDVEISRIASGIPIGTELEFADGVTLTESIKGRVHLTKE"
     gene            complement(2285..2587)
                     /locus_tag="PK717_03385"
     CDS             complement(2285..2587)
                     /locus_tag="PK717_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOR46738.1"
                     /translation="MGNNMFDMIKQASQMKAQVMKIEKELAAMKIEGESSKGEVKITA
                     LVNGKLDVIGLSITESEETKKLDIKDLTKLVLGAITKAQSEAKNEAAKIARNLQLG"
     gene            complement(2595..4187)
                     /gene="dnaX"
                     /locus_tag="PK717_03390"
     CDS             complement(2595..4187)
                     /gene="dnaX"
                     /locus_tag="PK717_03390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR46739.1"
                     /translation="MSSAYISLYRKWRSQSLDEIVGQEFAVRTLNNTFKSGRIAHAYL
                     FCGPRGVGKTSLARILAKSVNCETGITPAPCQTCQSCKSITDGTNLDVIEIDAASNRG
                     VDDIRQLRERVKFAPVSSRFKVYIIDEVHMLTGEAFNALLKTLEEPPPHVIFVLATTE
                     PHKIPVTILSRCIRFDLKRIPTDMQVQLLRRISDAEGIKITDDALRLLAIDSNGSLRD
                     AESLLDQVASFTDGEIGTEDIAMMLGVTGEKFLHKYLLHIAEGKAGEAIGLVREAYKN
                     GKEPEQISKDLLLRVHILILSAIGVSKQELLSDYAVDPELISQESSSFSFSSLRLIEM
                     KLRELVSQMRYTFSALASLEMTTLELSDIASGAVLQLPEQAPQAVKEQAPKTQIKKED
                     VLVAPMKEQTQKDPEVKPSVAQDSKPKAETSSCSCLADKVKEAVKETDIILFAFLTRL
                     LDVIIEGNIAQLILPSDAEFEKARLSERPNQEKLSTIVSKLIGQEVTAKPQLLKVQTK
                     NLAEDDQVKAAFDLFKGKIQEN"
     gene            4320..>6221
                     /locus_tag="PK717_03395"
     CDS             4320..>6221
                     /locus_tag="PK717_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46740.1"
                     /translation="MIILAIGLSGCMSGTKKQEDDIQKNSKNAPETEQQGDKSDLDAL
                     KNMYGTNLVSINNNPPVCIQAIPEIPKDDFYWKNKNVGNIKKNNEYKTSIEYSQYKAA
                     TLGNGILKWINDSKTQESFIYYWTRSNMAVCFFSSDNGQPFGTMDTNKPEFDTTISGK
                     LIFFFLNTEKEFYWYELSRNVFPLTKEVPFSIFDIDNNGMEEVHFRHDENIYEIIGIG
                     NRPVEFDLIMSVDINNVNNIYEKNNFPIIKTYKEYGTGDCIMAIKIGSVAGFVCPDYE
                     SRIHLVKISGEDLIDVSYIFPNILRGLWTPERLRNPFEKYIFLEQIGPLNQYKRNALT
                     SKIKEKREIEIVETLARFSLPLQAGDSYEHEKWYNREVWLHMDNFRYNKFPNYKDAWK
                     TLKEAINKKTVLSYEEVEELLFFNDSKNMIFIHSPYQDKPFEGRSWEIEPGSYWPERR
                     LGKHEMAKINYNNKEDLPQIKPESSQRDLIKFVNFTLGGKELTWNRGKFIADERNYMV
                     IPVSEDIIFIITDYPPCMTAIKLGNEKNNYKPTQLMDWIEGEMAILVNGIGDVNCDGI
                     TDFVFQFQNFGATENTNDTVTFSVINGQLHQLVMDVEGLGSVEYFFGSFGGRGHYGTL
                     ELGCAPVPDD"
BASE COUNT         1704 a         1407 c         1390 g         1720 t
ORIGIN      
        1 atgctgttga gtttttggcc agaaggtcag agggtggcgc aacagtaatg gcactgccaa
       61 gcctgcatgt gacctcgttg atgtgggaaa gggtcatgtg ctggtcttca acggttgtga
      121 gatagaaaac aggatagcct tcagtgacga tggcaagcgg gccatgcttg aactctgccg
      181 aatagagccc ttcacaatgg gcataggaga tttccttgat cttctgtgcg gcctcaagcg
      241 cagaaccata ggtcgcgcca tagccaagcg tgtacatgtc aacgcaatcc ttgtattttt
      301 cggcaagttc cttggcccag ccgttcatct ggtcgatggt gcccttgatt gcttttggcg
      361 ttttggcaag cgtttccgga agatcaccag ccctgccttt gagctttgca acttcaatgg
      421 caagcgccag gaaaagaact gactggttga taaaagtctt tgttgctgca acagctattt
      481 caagattgga gagcaatgga aggtgccttc tggagcgcat catgagggta gagcccagaa
      541 cgttaacaac cccaaggaca ttgtttgcca ttcctctggc agtaagcatg ttcaacacgt
      601 ttatgacgtc ttttgtttcg cctgattgtg agacaaggac aaaaaggtca gtgggtctta
      661 ttccttcacc aaaatgttcg ataaatgacc ctgcaacaac cgggaaagtt gcgcacccgc
      721 caagcttgtt gaggtagtac gatcctatta cacaagcatt gtacgacgaa ccggagccaa
      781 cgaggtatac tctgtcacca gaagctattt cttctgcaaa cgggacaagc tcatcacatt
      841 cggcaagata gtcaagcagg gcctgggccc tttctggctg ttcaaatatc tctttctcca
      901 tgaaaaacgg aaaatcgcct tttgaggccg actctgggga aagaggtgaa gtgattggtt
      961 ttctgtctat cttctcgcca ttctcaatgt tgcggatctc gtaagaatcg ctgtcaaact
     1021 cgacgtactc gccatcattg agtggaacta tctggtttgt gagattgagt attgacggca
     1081 aatcggagga acagcaaaca aatccgtcac cgactcccag ataaagtgat gaacccatct
     1141 ttgtgcaaaa catcttctcg cccttgagcg ctgtgatcgc gtaagcgtag tcgcccttga
     1201 gtatctttct tgcttccctg attgccagat cgagatctcc atggcgattg aatgagtctt
     1261 ctattacatg aaccacgacc tcgccatcgt tctcacccct gaacacatga ccctttgctg
     1321 tgagttcttt gatgagatgt atcgtattca caacgtttcc gttgtgggca ccaacaagcc
     1381 ttccttttgt gtcaaaatgc ggctgggcat ttctctgcgc agggactccg aacgtagccc
     1441 agcggagctg ggttatgccc ctgttgccac aaagctcctc aaaattgagc tttttggcaa
     1501 cctcatccac tttaccgata tcttttctga ggtcagcttt gccggttttc gttacagcaa
     1561 cggcacccac cgaatcatag cccctatagg taaggctttt tgcggccttc gtgagtgtct
     1621 tgcccatatc atccttgtct tctttgtaaa caatgccaaa aattccgcac atggttttct
     1681 tactccttgg ttagatgcac cctgcccttt atggattctg tcagggtcac accgtctgca
     1741 aattcaagct cggtgccgat cgggatgcca gaggcaatcc tggagatttc gacatcaatc
     1801 ccgtcaagct ctttttttat gtacattgct gtcaaattac cttcatgggt caggtttgtg
     1861 gcaaggataa tttcctttat cccaccattc ctcactctgg caatcaggcc gtctatatta
     1921 agtttttcag gcccaatacc cgatgcaagc ctaattgttc cgccaataac gtgataaact
     1981 cctgaaaatg aaccagccct ttcaatagcc aggacatcct ttgcttcttc aacaacaagc
     2041 acgattgaat ggtctctctt ttcatcaagg caaaccggac attcaccacc atccgaaaga
     2101 ttaaagcaaa ctggacagcg ctttacacgc tcatgcagtt tcccgattgc atcagagatt
     2161 ttcctggcct cttccgggtc catccccaaa ataaacagag acagcctttc ggctgttttt
     2221 gggccaatgc ctggcagctt gtggaggttg tcaacaagct cttgaaaggc cttgaccgga
     2281 tagttcatcc aagttggagg ttgcgggcaa tctttgcggc ctcgttcttt gcctcggact
     2341 gggcctttgt gattgcgcca agaaccagtt ttgtcaaatc ttttatgtca agtttcttgg
     2401 tttcttcgga ctcggtgatt gatagaccta tgacatcaag cttgccattt accaatgcgg
     2461 tgatcttcac ttcacccttt gatgactcgc cttcaatttt cattgcggca agctcttttt
     2521 cgatcttcat tacctgtgcc ttcatctggg aggcctgttt tatcatgtca aacatgttgt
     2581 ttcccatttt ctcttcaatt ctcctgtatt tttcctttga acaaatcaaa agcagctttt
     2641 acctggtcat cttcagccag gttttttgtc tgcactttga gcaattgtgg tttggcagtg
     2701 acttcctgcc caatcaattt tgacactatt gttgaaagtt tttcctggtt cgggcgctcc
     2761 gaaagccttg ctttctcaaa ttcggcatcc gatggcaaaa taagctgggc aatattgcct
     2821 tcaattatca catccagaag cctcgtcagg aaggcaaaaa gaataatatc agtttccttg
     2881 actgcttcct tgaccttgtc tgcaaggcag gagcatgagc tggtttcagc ttttggtttg
     2941 gaatcctgtg ccacagaagg cttcacttca gggtcttttt gggtctgctc tttcatcgga
     3001 gcaacaagca catcttcttt tttgatctgg gtttttggtg cttgctcttt tacggcctgc
     3061 ggagcctgtt ccggcaattg caaaaccgct ccagaggcga tatcggaaag ttcgagtgtc
     3121 gtcatctcga gggaagccag tgccgagaat gtatagcgca tctgagatac aagctccctg
     3181 agcttcattt ctatgagtct taacgaactg aagctgaagg acgatgattc ctgggaaatg
     3241 agttctggat caacagcata gtctgagagc agttcctgct ttgagacccc aatggcagag
     3301 agaatgagaa tgtgaaccct caagagtagg tcttttgaaa tctgctccgg ctccttgcca
     3361 tttttatatg cttccctgac caacccaatc gcttcgccgg ccttgccctc tgcaatatgc
     3421 agaaggtact tgtgcaggaa tttttcaccg gtcacgccaa gcatcatggc tatgtcctcg
     3481 gtgcctattt caccatcagt aaaagaagcc acctgatcaa gcaggctctc ggcgtccctg
     3541 agggaaccgt tgctgtcaat ggcaagcaac ctcagggcat catcggtaat ctttatcccc
     3601 tcggcatcag aaatcctgcg caacagctgg acttgcatgt cagtcgggat cctcttcagg
     3661 tcaaacctga tgcaccttga gagtattgtg accggtatct tgtgcggctc ggtggtggca
     3721 agcacaaaaa tcacatgcgg cggcggctct tccagcgtct tgagcagggc attgaatgcc
     3781 tcgcccgtga gcatatggac ttcgtcgata atgtagacct tgaaccttga ggaaaccggt
     3841 gcgaatttca ccctttccct caactgcctt atatcatcaa cacccctgtt tgaagcggca
     3901 tcgatctcga ttacatcaag gtttgtcccg tcagtgattg acttgcaact ctggcatgtc
     3961 tggcaggggg ccggagtaat gcctgtttca caattgacag atttggccag tatcctggca
     4021 agactggtct tgccaacgcc ccttggccca caaaaaaggt aggcatgagc aattcttcca
     4081 gacttgaacg tgttgttcag ggtcctgact gcgaactcct ggccaacgat ctcgtccagc
     4141 gactgcgaac gccatttgcg atagagagaa atatatgccg atgacacgaa agagagtgta
     4201 gtttggggca actttcaagt caaggaacca agggcttttt gtctctctat cattgcaaac
     4261 cggcaccctt tatagtatat ggaacctgga gggcaggaat gaagaaactg gcgcttatta
     4321 tgatcattct ggcaattggg ctgtcagggt gtatgtctgg gacaaaaaag caggaagatg
     4381 atatacaaaa aaacagcaaa aatgcccccg aaacagaaca gcaaggtgac aaaagtgact
     4441 tggatgcttt aaaaaatatg tacggaacta atttggtatc tattaataat aatcctccag
     4501 tgtgtattca agctatccca gaaatcccaa aagatgattt ttactggaag aacaaaaatg
     4561 tcggaaatat aaagaaaaac aacgaataca aaacttcgat tgaatacagc caatacaaag
     4621 cggccacact tggcaatgga attttaaaat ggatcaatga cagtaaaact caagaatctt
     4681 ttatttatta ttggaccagg agcaacatgg cagtttgctt tttctcatct gataacggcc
     4741 aaccatttgg gacaatggac actaacaagc ccgaatttga cacaacaatc tccggtaaac
     4801 ttattttctt ttttttgaac actgagaaag aattttactg gtacgaattg tccaggaatg
     4861 tttttccatt aacaaaagag gtacccttct ccatattcga tattgacaac aatgggatgg
     4921 aggaagttca tttcagacat gatgaaaaca tatatgaaat aatcggcata ggaaataggc
     4981 ccgtagaatt cgatctaata atgagcgtgg atataaacaa tgtaaacaat atctacgaaa
     5041 aaaataattt tccgataatt aaaacataca aagagtatgg aaccggtgat tgcataatgg
     5101 cgatcaaaat cgggtctgtg gctggttttg tatgccctga ctatgagtcc agaattcacc
     5161 tagtcaaaat ctccggggaa gatttgatag acgtttccta tatattccca aacatcttga
     5221 gagggctgtg gacaccagag aggctaagaa atccttttga aaaatacatt ttccttgagc
     5281 agatagggcc attgaatcaa tacaaacgca atgcgctaac aagcaaaatc aaagaaaaac
     5341 gggaaataga aattgtcgaa acacttgcca gattttcatt acccttgcaa gcaggagatt
     5401 catacgaaca cgaaaaatgg tataaccgtg aagtgtggct ccatatggat aattttagat
     5461 acaacaagtt tccaaattac aaagatgcct ggaaaacttt gaaggaagct ataaataaaa
     5521 aaaccgttct atcttacgag gaagttgaag aactactatt ttttaatgat tcaaaaaata
     5581 tgatttttat ccattcgcca tatcaagata aaccttttga aggcaggagc tgggagattg
     5641 aacctggaag ctactggcca gaaagaaggc ttggaaaaca tgagatggca aagataaatt
     5701 ataacaacaa ggaagatttg ccacagataa aaccagaaag ttctcaaaga gatctcatca
     5761 aatttgtgaa tttcacgctt ggaggtaaag agcttacatg gaatcgaggc aaatttatag
     5821 cagatgagag gaattacatg gtaattccag tttcagagga tataattttc attataaccg
     5881 attacccgcc atgcatgact gccatcaaac ttgggaatga aaaaaataat tataaaccaa
     5941 cccaattgat ggactggatc gagggagaaa tggcaatctt agtcaatggg ataggcgatg
     6001 taaattgcga tgggattaca gacttcgttt ttcaattcca gaattttggt gcgactgaaa
     6061 acaccaatga tacagtaaca ttctcggtaa taaacgggca attgcaccag cttgtgatgg
     6121 atgttgaagg attgggatcg gttgaatatt tctttgggtc tttcggtggc aggggccatt
     6181 atggcaccct tgagctaggg tgcgcaccag ttcctgatga t
//
LOCUS       DAOSRI010000080         6058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_11951_length_6058_cov_3.738797, whole genome shotgun sequence.
ACCESSION   DAOSRI010000080 DAOSRI010000000
VERSION     DAOSRI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_6058_cov_3.738797"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PK717_03400"
     CDS             <1..1036
                     /locus_tag="PK717_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOR46741.1"
                     /translation="LFTPPESGLVNPDNLYIMVSHVKCAAFELPFVDGESFGKAEIEP
                     ILSHFEKEGIVNHDQGKWFWMTDSYPADEISLRNATEGNVAIIDVTGSPHVRTIGEID
                     LFSAPMLVHDQAIYMHDGVTYHVDRLDFKERKAFVRKVPVDWYTDANLQISLRVLSEA
                     ENNDIGNCKLGAGEVTVTALTTVFKKIQPTTHQNLGYGEVHLPEMPMDTMSWWVTVGE
                     KIVLEFSKNDLEKALKGLGNILIGIMPLPLMCDPRDLGMYVEVKSPHFEAPTVFIYER
                     VAGGVGLSEHMFDMGISPLVLALDTLQKCPCENGCPSCVGPAGDSELGTKLQTVRLLK
                     MLTAGRPGPA"
     gene            complement(1008..1388)
                     /locus_tag="PK717_03405"
     CDS             complement(1008..1388)
                     /locus_tag="PK717_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOR46742.1"
                     /translation="MEREEFDLIVEQCWNELPEEWKKELEEANISYEIIDLATNEIAA
                     KAGTSPWNLLGLFSGVPKTVRHKGFEPMAFPDRITVFRLPIMSRVFDIENLRKTIKHV
                     LYHEIGHYFGLSEDELRQAQGDPL"
     gene            complement(1389..2282)
                     /locus_tag="PK717_03410"
     CDS             complement(1389..2282)
                     /locus_tag="PK717_03410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46743.1"
                     /translation="MLLDWVFCPYNSKGAFAREAEACFDLVLRRWDITQIRDASISLL
                     PKHIAGCIKSVRSNNERKETQGAKPCFFLEGEELPCYQNIHDFFAAMEQRNMARSREP
                     VINELFVHPRLPWERRDQHVLVLPVTAKDIAGYGIPTPSMKEDVRFGDIGNPETLEFM
                     TLTTRNYGKMMSIAFIGSDVAGYITHVPKPLANRMGCKFVDEIQDKGIMQIIDLYVYP
                     QFRGEGVEKSLIANLAEFCRKNRHQKIEVYTLETPLEDYMPSTGHSAMYDGSGFKTKK
                     SIKQKNQENKEYDFVLMTMET"
     gene            complement(2301..3281)
                     /locus_tag="PK717_03415"
     CDS             complement(2301..3281)
                     /locus_tag="PK717_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOR46744.1"
                     /translation="MKIVFDVKTAGPFHSMPEWALSETRKALPAHEIFSVTRDELPSY
                     VENADVIVCYSFPRELFLQAHNLKMLVFAVDGIGPQRLYPELRNSQVPVINSKGCRSQ
                     AIAEHAFSLLLACSRKIVPMGKTLTPEGWWGNGVTEGHPPMEISGKTIGILGLGHIGK
                     RVAKIAKNGFDMRVLGMRHSKGDVENVDEVFGPDGLDHILEKSDVIILCLPETENTQC
                     LIGQKEFALMKHGVIIINIARGSLIDTQELVGAIKSGKVAAAGLDVFEKEPLPADSAL
                     RTLPQIVATPHAAGVTPHFWERFIKIIIENVKRLESGEQLLNITDKVLGY"
     gene            complement(3291..5018)
                     /gene="rlmB"
                     /locus_tag="PK717_03420"
     CDS             complement(3291..5018)
                     /gene="rlmB"
                     /locus_tag="PK717_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR46745.1"
                     /translation="MENERSRDRKGGFGKSNFRDNGPSKRSFGDRKPFGERKSFGDRK
                     PYGDRKPYGENKPFGERRSYGDRKPYGEGRPFGERKPYGDRKPYGENKPFGERRSYGD
                     RKPYGENKPFGERRSYGDRKPYGEGRPFGERKPYGDRKPYGENKPFGERKPYGERRSY
                     GDRKPYGDKPYEPRKRFGEDRPFGERKPYGDRKPYGENKPFGERRSYGDRKPYGEGRP
                     FGERKPYERRPPRGDSDSDGSPKLSWQKKEWSGPSDRRFGRRDDEGGHRPRFPRQDGD
                     GEFRREHIYERPPSRTGSKFQRRPFEEREVQDLEYAEELPCANEVTLEEADNAIIGKN
                     AVLEAIRAGTSINKVLIRKTLKINTVLKAIMDESKDRGIRYDLVSDFEIGKYGSRDVV
                     ALASPTQYAEIEDLISPENTISMIAILDGIEDPQNLGAIIRSSECFGATGVIIPKHGA
                     APVTSTVTKTSAGAALRQKIARVANIKIAIEKLKEAGYWIYGSSPDAISESQSVEFPD
                     KCCFIIGSEGDGMHKTVAEHCDEIIKIPMKGKLGSLNASVAAAILMYEWAKKHPQPDE
                     KVDQPTIEH"
     gene            complement(5061..>6058)
                     /locus_tag="PK717_03425"
     CDS             complement(5061..>6058)
                     /locus_tag="PK717_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR46746.1"
                     /translation="RAADNSIYYSISSFKDYGKLAHLEINTLQSGASGRVASDEYEKD
                     SISDFALWKAWDENDGEVYWETQLGKGRPGWHIECSAMSMEYLGETLDIHTGGIDNIF
                     PHHQNEIAQSEGFTGKTFSKYWMHSYHLMVDGAKMSKSLGNFYTLEDLEKKGISPETF
                     RFFVVTNHYRTSLNYTDEAARAAGKARDSLIEFVDRVRNIPDGPDKFDVGPTIEEMFR
                     EFEAGMDDDLNTPQAIAAVFDMTRPINRAIDEGKIGTLGRENLLKTIEKVNTVFAFIE
                     GSQTTIPEEVIELMKQREQVRKEKNWAMSDQLRDKILELGFIVKDTPQGQRVTKK"
BASE COUNT         1291 a         1499 c         1366 g         1902 t
ORIGIN      
        1 gctgttcaca cctccggagt caggtttggt aaacccggat aacctctaca ttatggtctc
       61 ccatgtgaaa tgtgcagcat tcgagcttcc ttttgtggat ggagaatctt ttggcaaggc
      121 ggagattgaa ccaatccttt cgcactttga aaaggagggg attgtcaatc acgatcaggg
      181 gaaatggttc tggatgacag attcttaccc tgctgatgaa atatcgctcc ggaatgcgac
      241 cgaagggaat gttgccataa tcgatgttac aggctccccg cacgtcagga ctattggtga
      301 aatagatttg ttttctgcac caatgcttgt gcatgaccag gcaatctaca tgcacgacgg
      361 ggtcacatat cacgttgacc ggcttgactt caaagagaga aaagcttttg tcagaaaagt
      421 tccggttgac tggtatacag acgcaaattt gcagatcagc ctcagggtac tctcggaagc
      481 tgaaaataat gatattggaa actgcaagct tggtgcaggt gaggtcaccg taacggcatt
      541 gacgacagtg ttcaaaaaaa tccagccgac gacccaccag aaccttggct atggtgaagt
      601 gcacctcccg gaaatgccga tggacaccat gtcatggtgg gtgactgttg gcgagaagat
      661 tgttttggaa ttctcgaaaa atgatcttga aaaggcacta aaagggcttg ggaacatcct
      721 gattggaatc atgccgttgc cgcttatgtg tgaccccagg gacttgggga tgtatgttga
      781 ggtaaaatca ccccattttg aggcgccaac cgttttcatc tatgaaaggg tggctggagg
      841 ggtcggtctt tcggagcaca tgtttgatat ggggatttca ccgcttgtgc ttgcactcga
      901 tacgctccag aagtgtcctt gcgagaatgg ttgcccgtca tgtgttggtc ctgccggtga
      961 ttcggagctt ggaaccaagc tccagacggt aagacttttg aaaatgctta cagcgggtcg
     1021 ccctgggcct gcctgagctc gtcctctgac agaccgaagt agtgtcctat ttcgtgatac
     1081 aggacatgtt ttatcgtctt cctgagattt tcaatgtcaa aaactcttga catgattggc
     1141 aacctgaaaa cggttattct gtcaggaaaa gccattggct caaaaccctt gtgcctgact
     1201 gtttttggga ccccggagaa tagtcctagc aggttccatg gactcgttcc ggcttttgcc
     1261 gcgatttcat ttgttgccag gtcgattatc tcgtaggaga tgtttgcttc ctcaagttcc
     1321 tttttccatt cttctggcag ttcattccag cattgttcga caatcaggtc aaattcttca
     1381 cgttccactc aggtctccat ggtcatcagg acaaagtcat attctttgtt ttcctggttt
     1441 ttttgtttta tggatttttt tgtcttgaac cctgaaccat catacattgc cgagtggccg
     1501 gttgatggca tgtaatcttc caaaggtgtc tccagtgtgt agacttctat cttttgatgt
     1561 ctgttttttc tgcaaaattc ggcaaggttg gcaatcagtg atttttcaac gccctctccc
     1621 ctaaattgcg gatacacgta aaggtcaata atttgcatga tacccttgtc ctgtatctcg
     1681 tccacaaatt tgcagcccat tctgttggcc agaggttttg gcacatgtgt tatgtatccg
     1741 gcaacatcag aaccaataaa agcgatggac atcattttgc cataatttct cgttgtgagc
     1801 gtcatgaatt cgagtgtttc agggtttccg atatcgccaa acctgacgtc ttccttcatg
     1861 cttggtgttg gaatgccgta ccctgcaata tcttttgctg tgacaggcaa cacaagaacg
     1921 tgctggtctc tcctctccca cgggagtctt gggtgaacaa acagttcgtt gatgacaggt
     1981 tctctgctcc ttgccatgtt tctttgttcc attgctgcaa aaaaatcatg gatattctgg
     2041 tagcagggga gttcttcacc ctcgaggaaa aaacacggtt tggcaccctg ggtttctttt
     2101 ctttcgttgt ttgatcttac tgattttatg caaccggcaa tatgttttgg caacagtgag
     2161 attgaggcgt cccttatctg tgttatgtcc catctgcgca agaccagatc aaaacaggcc
     2221 tcggcctccc tggcaaaagc accttttgaa ttgtaggggc agaaaaccca gtcaaggagc
     2281 atggtcaccg ctttcctggc ttaataccca agcactttgt cagtaatatt aagcagttgc
     2341 tccccagact caagtctttt gacgttctcg atgattattt tgatgaacct ttcccagaaa
     2401 tgtggtgtga cacctgcagc gtgtggagtt gccacgatct gcgggagtgt cctcagagct
     2461 gagtctgcag gcagtggctc tttttcaaac acatcgagcc ctgcagctgc cactttgcca
     2521 ctttttatgg cacccaccag ctcctgcgtg tcaatgaggg agcccctggc aatatttatg
     2581 atgatcaccc catgcttcat gagtgcaaat tctttttgcc caataagaca ttgggtgttc
     2641 tctgtttcag ggagacacaa tataattacg tcggatttct cgagtatgtg atcaagccca
     2701 tctggcccaa aaacctcgtc cacattttca acgtcccctt tgctgtgcct catcccaagg
     2761 actctcatgt caaagccatt cttggctatt tttgcaaccc tcttgccgat atgacccagg
     2821 ccgagtattc cgatggtttt ccctgagatc tccatcggtg ggtgaccttc tgtcacccca
     2881 ttcccccacc atccttcagg tgtgagcgtc ttgcccattg ggactatttt tctggagcat
     2941 gcgagaagta gtgaaaatgc gtgttctgca atggcctggc tcctgcaacc ttttgaattt
     3001 atgacaggta cctgtgaatt tctcagttcc ggatagagcc tttgcgggcc aatgccatca
     3061 acagcaaata caagcatttt gagattgtgg gcctgaagaa aaagctccct tgggaatgaa
     3121 tagcatacga taacatctgc attttccaca taacttggga gctcatcacg cgttacagaa
     3181 aatatttcgt gcgccggaag ggccttcctt gtttcagaaa gcgcccattc cggcatcgag
     3241 tgaaacggac ctgccgtctt cacatcgaag actattttca tagattgatt ctaatgctct
     3301 attgtgggtt ggtcaacctt ttcgtctggc tgtgggtgtt tttttgccca ctcgtacatg
     3361 aggattgctg cagcaactga agcattcagc gaaccgagtt ttcccttcat ggggatcttg
     3421 attatctcgt cgcagtgttc tgcaactgtc ttgtgcatgc catcaccttc ggagccaatg
     3481 atgaagcagc acttgtcagg aaattccact gattgtgatt ccgagatggc atctggtgag
     3541 gaaccataaa tccagtatcc tgcttctttg agtttttcaa tcgctatctt gatgtttgcc
     3601 accctggcaa ttttttgcct gagtgcggca cctgcagagg tttttgtcac agttgaagtg
     3661 accggtgcgg cgccatgttt cgggattatg acgcctgtgg caccgaagca ctcggaagac
     3721 cttatgattg cgccaagatt ttgcggatct tcgatcccgt cgaggatagc gatcattgat
     3781 atggtgttct ctggactaat tagatcttcg atttcagcgt attgcgttgg cgaggcgagg
     3841 gcaaccacgt ccctgctgcc atatttgccg atttcaaaat cagaaaccag gtcgtatctt
     3901 atgcctctgt ccttactctc gtccatgatt gctttcagga cagtgttgat cttgagggtc
     3961 ttcctgatga ggaccttgtt gatgctggtg cctgccctga ttgcttcaag cacggcgttt
     4021 ttgcctatga ttgcgttgtc tgcctcttcc agggttactt catttgcaca cgggagctcc
     4081 tcggcatatt ccaggtcctg gacttccctt tcttcaaagg gtcttctttg aaacttgctt
     4141 cctgtgcgtg atggtggtct ctcatatatg tgttctctcc tgaattcgcc atcgccatcc
     4201 tgccttggga accttggcct gtggccaccc tcgtcatctc ttctgccaaa ccttctgtct
     4261 gatgggcctg accattcttt cttttgccag gaaagttttg ggcttccatc actatcagaa
     4321 tcgccccttg gtggtcttct ctcataaggt tttctctcgc caaaaggcct gccttcacca
     4381 tacggcttcc tatcgccgta tgatcttctc tcaccgaaag gcttgttttc tccgtacggc
     4441 tttctgtcac catagggttt tctttcacca aaaggcctgt cttctccaaa tcttttcctt
     4501 ggttcgtagg gtttgtctcc ataaggtttt ctatcaccat acgatcttct ttcaccgtag
     4561 ggttttctct cgccaaatgg cttgttttcc ccatatggtt ttctgtctcc atagggtttt
     4621 ctttcgccaa aaggtctgcc ttcaccgtat ggttttctat caccatacga tcttctttca
     4681 ccaaaaggct tgttttctcc atacggtttc ctatcgccat atgatcttct ctcaccgaat
     4741 ggcttgtttt ccccatatgg ttttctgtct ccatagggtt ttctttcgcc aaaaggtctg
     4801 ccttcaccgt atggttttct atcaccatac gatcttcttt caccaaaagg cttgttttct
     4861 ccatacggtt tcctatcgcc gtatggcttt ctgtctccaa aagactttct ttcaccaaaa
     4921 ggctttctgt ctccaaaaga tctttttgat ggaccgttgt ccctgaaatt gctttttcca
     4981 aagccgcctt ttctgtcgcg gctcctttcg ttttccactt taaattcgct ttctgccctc
     5041 tttggggctc ttgtatggcc ttattttttt gtcacgcgct ggccttgggg cgtatccttt
     5101 acgatgaaac cgagctccag tattttatcc ctgagctggt ccgacatggc ccaatttttt
     5161 tcttttctca cttgctccct ttgcttcatc agctctataa cttcttccgg aatagtggtt
     5221 tgcgagcctt caataaatgc aaagacggtg tttacctttt cgatggtctt taagaggttt
     5281 tcccttccca gtgtgccgat cttgccttca tctattgccc tgtttatagg gcgtgtcatg
     5341 tcaaataccg cggcaattgc ctgcggtgtg ttcaggtcat catccatccc ggcttcaaac
     5401 tccctgaaca tctcttcgat tgttggccca acatcaaatt tatcggggcc atcgggtatg
     5461 tttctcaccc tgtcgacaaa ttcaataagg ctgtctcttg cctttccagc ggcccttgcg
     5521 gcctcatcag tgtaatttag acttgttctg tagtggtttg ttacaacgaa gaagcggaat
     5581 gtttccggag atattccttt cttctcaagg tcctccagcg tgtagaaatt cccaagcgat
     5641 ttgctcattt ttgcgccatc aaccatgaga tggtacgagt gcatccagta ttttgagaac
     5701 gtcttgccgg tgaaaccctc cgactgtgca atctcgttct gatggtgtgg gaagatgttg
     5761 tcaatgccac ctgtgtggat gtcgagggtc tcgccgaggt attccatcga catcgcagag
     5821 cactctatat gccagccggg acgtcccttc ccaagctggg tctcccaata tacttccccg
     5881 tcgttttcgt cccatgcttt ccataaggca aaatcagaga tggaatcttt ttcgtattcg
     5941 tcagaagcca ctcttcctga tgctcccgat tgaagagtgt ttatttcaag atgggcaagt
     6001 ttgccataat ctttaaaaga ggagattgaa taatagatcg agttgtcagc tgccctgt
//
LOCUS       DAOSRI010000081         5978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12136_length_5978_cov_4.020260, whole genome shotgun sequence.
ACCESSION   DAOSRI010000081 DAOSRI010000000
VERSION     DAOSRI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12136_length_5978_cov_4.020260"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3725)
                     /locus_tag="PK717_03430"
     CDS             complement(<1..3725)
                     /locus_tag="PK717_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR46747.1"
                     /translation="MMKRFASLTLVIMMFATLLPLASQVQASDYSFAGLIEKIASEAP
                     MKYIEKLSSKEFRGRMVGDPGEKITTDYIASLYKSFGLKPAGDDDTYFQNFSTSVFLP
                     KSPVDFRMVTEDGGTEKQFVYKKDYNFLQVTGSGKANADAIFAGFGISTQKYNYDDYK
                     GIDAKGKVVAVIRHAPDYLRELAGQEADSLMAFQYKVDNAIAHGASAVVVFENPMLPP
                     ENRRNMNEKIVSAYPSSIPCFFVSVECSNQILSKSGLTSEQLFKKINDSKSPSSFQIK
                     NTRLQVEVNAEFNPAHKTSNVVGYIAASDPFGANETVLITAHYDHLGVDVDGTLYPGA
                     CDNASGIASMLEIARVFTDSGLKPKINICFAALSGEELGLVGASYMANNPPFPLDGMT
                     VLNLDMVCGEPNRINLSMDANFTDLFDKLKFSGLKVGADVRQGRPANNNDSAAFYAKG
                     VPSVFIFGGRGMMWHDPEDTIEHVNPEGLQLCTRFAALTACLYAEPFFLALDSQSLES
                     PAPDYVVSGYTGRGARVTIGGRQTIASETGKFSIPVTLGEGKNDLKVTATKPSSGEKI
                     EKTITVSYVLQAKAELSTSMLNFGFVTQRSEPKTITFTITNTGKGPLSGTLKPCSDWL
                     EIKPAKLNPDKTVVEATLLPKWLIPKGFHICMIQIETSGGMVFLPVTAVTGEEPTVEL
                     IMTPGSKRVKIQGDDVPVSQGFEVVSNANCYPSSALSMSFGMLVKKDADGIKLTFNDE
                     QYRLWTGSAVGLKGNVPFTLKKEVYENKDEVMLPESLLEIMGMGIEKKEGGQVVITYD
                     MSKIAPFSAEIFPPDFIELEQDTIYSKDFSWMTRQSFKFTPNRSGLLSYIVPDWLHLG
                     NNYSKNYPVEGGKTIEIWPFPNNALINQLKPKSGKIEFFINGHPVGSVEVKLKYANTK
                     RIISIVIGSKTATIDGEEKEVSPPAFIEKGTTYVPYRFIGEPFGAKVEWVQQTRSVRA
                     TIGTTTIELTINSKKAIINGVPTTLQSPAIIKDGRTFVPIRFIGEAFGADVSWDQKTK
                     TVLVTLDLAKDKPLVDVDKNNIDIQWHDATPNAIPPKTTVTIINKGGGKIIFKEPIDT
                     TSAMQTTRVSVSKEKVTLTPIFGSDLPIETSDAAIVATYNGIVGLNLNVGIYPKDSVL
                     FSSKGDFYKINGFEQQDQPKVLDGLVSTSVLKFISSCGGSSIFDPTSGNLAIMMRGKT
                     LELGTKTGDFFLDGTKMPWKFRYEVKGEDVMLPWACLS"
     gene            complement(3722..4933)
                     /locus_tag="PK717_03435"
     CDS             complement(3722..4933)
                     /locus_tag="PK717_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR46748.1"
                     /translation="MSKIDPCRLRRDFTVLDRENPPIYLDNACMTLKPKQVVDAMTTY
                     YDEYPVCEGRSAHAMAMRLEQDMLASRRAIKDYIKANHVNEVVFTRNTSESISIVSYG
                     IDWKPGDIVLVSDKEHNSNFVPWFRLKEWGVKVIVVPSGDDGRFSVDNWAKAIKESKP
                     RLISFYHTSNLDGETSRVAEVCKLGKEAGALVCLDAAQGAAHVPLNVQEIGCDFMAIS
                     IHKMCGPTGMGALYGKLEELRKLKPLTTGGGTVFSVTYDEVKLLDPPRKFEAGLQNYA
                     GIVGVKPAIDYIESIGLSNIHEHEIELNQYLQDLLEGVPQIKILGPKDAKDRGGITSM
                     TVEGYNVHDLAMLLDDRVGIMLRAGFHCVHGWFNSRGIDGTLRVSTYLYNTKEEMEIF
                     ASAIKEIIAEE"
     gene            complement(4921..5133)
                     /locus_tag="PK717_03440"
     CDS             complement(4921..5133)
                     /locus_tag="PK717_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR46749.1"
                     /translation="MPIYEYKCPSCGHKFSELLSYNAKNPKCPKCGCENTKKMVSSFS
                     SPSSSANSSGSSCNSCNGGSCSSCQR"
     gene            complement(5120..5581)
                     /locus_tag="PK717_03445"
     CDS             complement(5120..5581)
                     /locus_tag="PK717_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46750.1"
                     /translation="MGIISDLENFRELILNGKKAAFSKTILIPEKEAREILERIIQDF
                     PVEFEEASEIIRKRADLIRQAEIEAESIKLNADERAKIIKEATEKAQEIVNQANFEAS
                     RIMEEAKTESKNMVCQTNDFVRKILEQAKTSITKSQAILEESIEGTKNANL"
     gene            complement(5565..>5978)
                     /gene="coaD"
                     /locus_tag="PK717_03450"
     CDS             complement(5565..>5978)
                     /gene="coaD"
                     /locus_tag="PK717_03450"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR46751.1"
                     /translation="PVWGCDSGCCQNIWQDPLLMYEQRINLLKQIFSGNLNVKIIGFT
                     GLLVDCAKKTNSNVIVRGLRTISDFDVEYRMSTINNVVMPELETVFLMSEPEYSFISS
                     TFVKQIAREGGDISAFVPSVVLELLKELEENGHNI"
BASE COUNT         1407 a         1366 c         1317 g         1888 t
ORIGIN      
        1 gaaaggcagg cccagggcag catgacatct tcacctttta cctcatacct gaacttccac
       61 ggcattttgg tgccatcaag gaagaaatca cctgtttttg tgccaagctc aagtgttttg
      121 cccctcatca ttatggcaag attgccgctt gttgggtcaa atatgcttga tccgccacag
      181 gaggaaatga atttgagaac agatgtcgag accagtccat caagcacctt tggctggtct
      241 tgttgctcaa agccgttgat tttgtaaaag tctccttttg acgagaaaag gaccgagtct
      301 tttgggtaaa tgccaacatt gaggtttaat cccacaattc cattgtaagt tgcaactatt
      361 gccgcgtcac ttgtttcaat gggcagatca cttccaaata ttggggtaag ggtaaccttt
      421 tcctttgaaa cagaaacgcg tgttgtttgc atggcacttg tggtatcaat gggttctttg
      481 aaaattattt tcccgccacc tttgtttatt attgtaaccg ttgtttttgg cggaattgcg
      541 tttggtgttg catcgtgcca ctgaatgtca atattgtttt tatctacatc cacaagtggt
      601 ttgtcttttg caaggtccaa tgttacaaga acagtctttg tcttctggtc ccagctcaca
      661 tctgcgccaa aggcctcgcc aataaatctt atcgggacga atgtccttcc atcttttatt
      721 atcgccgggc tctggagtgt tgttgggaca ccattgatga ttgccttttt tgaattgatg
      781 gtcagctcga ttgtggttgt gcctattgta gcacggacac tcctggtctg ttgcacccat
      841 tccacctttg caccgaaagg ttctccgata aacctgtatg gaacataggt ggttcctttt
      901 tctataaagg ccggcgggga gacttctttt tcctccccat caattgtggc cgtttttgat
      961 ccgattacaa tcgagattat cctttttgta tttgcgtatt tgagtttcac ttcaactgat
     1021 ccgacgggat gcccatttat aaaaaactca atttttccag attttggttt gagctgattt
     1081 atgagcgcat tattggggaa tggccatatt tcaattgttt tgccgccttc aacaggataa
     1141 tttttggaat agttgttacc taggtgcagc cagtccggaa caatgtagct taaaagccca
     1201 ctcctgtttg gtgtaaactt gaaagattgc cttgtcatcc aggaaaaatc tttggaatat
     1261 attgtgtctt gttcaagctc aatgaaatcc ggtgggaaga tctctgcgga gaatggggca
     1321 atctttgaca tgtcgtaggt gattacaacc tgaccgccct cttttttctc gattcccatg
     1381 cccatgatct caagaaggct ctcggggagc attacctcat ctttgttctc ataaacttct
     1441 tttttgagtg taaatggaac attgcctttc aatccgactg ccgaacctgt ccacaacctg
     1501 tactgctcgt cattgaaggt gagctttatc ccgtcagcat ctttttttac gagcattcca
     1561 aatgacatcg aaagagcaga tgacggatag caattggcat ttgatacgac ctcaaacccc
     1621 tgactcacag gtacgtcatc gccctggatt tttacccttt ttgaccctgg cgtcatgatg
     1681 agctctacag ttggctcctc gcctgtgact gcagttacag gcaggaaaac cattccaccc
     1741 gatgtttcaa tctggatcat gcatatatgg aagccctttg gaatgagcca ttttggcaat
     1801 agcgttgcct caacgactgt tttgtcggga ttgagtttgg ccggctttat ttcaagccag
     1861 tcggagcatg gtttcaatgt tccggaaagt ggacccttac ctgtgtttgt gattgtgaat
     1921 gtgattgttt ttggttctga tctctgggtc acaaaaccaa agttcagcat tgacgtggag
     1981 agttcagcct ttgcctggag aacgtatgaa acagtgattg ttttttcgat cttctcgccg
     2041 gatgatggtt ttgtggctgt gaccttcaaa tcattcttgc cttcgccaag tgttaccgga
     2101 atggaaaatt ttccagtttc agaagctatt gtctgccttc caccaatggt gacccttgcc
     2161 cccctcccag tgtaacctga cacaacgtag tcaggtgctg gagattcaag cgactggctg
     2221 tcaagcgcaa ggaagaatgg ttcggcataa aggcacgctg ttagagccgc aaaccttgtg
     2281 caaagctgga gaccctccgg attgacatgt tctatggtgt cttccggatc gtgccacatc
     2341 atgccccttc caccaaatat gaaaactgat gggacaccct ttgcatagaa tgccgcagaa
     2401 tcattgttgt ttgcaggcct tccctgtctt acatcggcac caacctttag tccggaaaat
     2461 ttcaatttat caaagagatc ggtgaaattg gcgtccattg acaggttgat cctgtttggt
     2521 tcgccacaga ccatgtcaag gttgagaact gtcattccat caagcgggaa aggtggattg
     2581 ttggccatat agcttgcccc gacaaggcca agttcttcac cggaaagcgc tgcaaaacag
     2641 atgtttattt ttggcttgag gccactgtct gtaaaaaccc ttgcgatttc aagcattgat
     2701 gcgatgcctg aggcattgtc gcatgcacct ggataaaggg tgccgtcaac atccacaccg
     2761 aggtggtcat aatgtgctgt tatgaggact gtttcatttg caccaaacgg gtctgatgct
     2821 gcaatatagc cgacgacatt ggaggtttta tgtgcaggat taaattctgc attgacttct
     2881 acctggagcc tcgtgttttt gatctgaaaa gaactcggag atttggaatc gttgattttt
     2941 ttgaagagct gttcggaagt caggcctgat tttgaaagta tctggtttga gcattccaca
     3001 gagacaaaaa agcatgggat ggaagaagga taggcactga cgatcttctc gttcatgttt
     3061 cttctgtttt ctggcgggag catcgggttc tcaaatacaa caacagcaga tgctccgtgg
     3121 gctattgcgt tgtctacctt gtattggaac gccattagcg aatctgcttc ctgtccagct
     3181 agctccctga gataatccgg agcgtgcctt attacggcaa caaccttgcc cttggcatcg
     3241 attcccttgt agtcgtcata attgtatttt tgagttgata ttccaaatcc tgcaaagatg
     3301 gcatctgcat ttgccttgcc tgagccagtc acctggagga aattgtaatc ttttttgtaa
     3361 acaaactgct tttccgttcc tccatcctct gtgaccattc tgaaatccac tggtgatttt
     3421 ggaagaaaaa cggaggtgga aaagttttgg aagtatgtgt cgtcgtcacc tgccggcttg
     3481 aggccaaatg atttgtataa tgaagcaatg tagtctgttg ttatcttctc tccgggatca
     3541 ccaaccattc tgcccctgaa ttcctttgac gagagttttt caatatattt catgggggct
     3601 tctgatgcta tcttttctat cagccccgca aaagagtagt ccgatgcctg cacctgactg
     3661 gcaagcggca gcagggttgc aaacatcatt attacaagtg ttaacgatgc aaatcttttc
     3721 atcattcctc cgcgattatc tctttgatgg cgctggcaaa tatttccatt tcttcttttg
     3781 tgttgtagag gtaagttgaa acccttagtg tgccgtcgat tccccttgaa ttgaaccagc
     3841 catgcacgca atggaagcct gccctcagca ttattccgac tctgtcgtca aggagcatgg
     3901 caaggtcgtg gacattgtag ccttcgaccg tcatacttgt tatcccaccc ctatccttgg
     3961 cgtcttttgg cccgaggatc tttatttgtg gaacaccctc aagaaggtct tgcaggtact
     4021 gattcagttc aatttcgtgc tcatgtatgt tggagagccc tatcgactcg atgtagtcta
     4081 tggctggctt tacacccaca ataccggcgt aattttgcag ccctgcttcg aattttcttg
     4141 gaggatcaag cagttttact tcatcgtatg ttacagaaaa cactgttcca ccaccagttg
     4201 ttagtggttt caatttcctt aattcctcaa gcttcccgta gagtgcgccc atgcctgtcg
     4261 gaccgcacat cttgtggatt gagatggcca tgaaatcaca gccaatttct tgaacattga
     4321 gtgggacatg tgccgctccc tgtgccgcat cgagacaaac cagtgcaccg gcctctttcc
     4381 caagcttgca cacctcggcc actcttgagg tttcaccatc aaggtttgaa gtgtggtaga
     4441 aacttatgag tcttggtttt gactctttta tcgcttttgc ccaattgtcg actgagaatc
     4501 ttccgtcatc tccggatggt acaacgatga cctttacacc ccattctttc agcctgaacc
     4561 atgggacaaa gtttgaattg tgttccttgt ctgaaaccag cacaatatcg ccaggtttcc
     4621 agtctatgcc atatgaaact atgcttattg attcggaagt gttccttgtg aacacaacct
     4681 cgtttacatg gtttgcttta atataatctt ttatggccct tcttgaggcg agcatgtcct
     4741 gctcgagcct cattgccatt gcatgggccg accttccctc gcagaccggg tactcgtcat
     4801 aataggtagt catcgcatcg acaacctgtt tgggttttag tgtcatacag gcgttgtcga
     4861 ggtaaatggg cgggttttcc ctgtccagga ctgtaaaatc tcttctgagt ctacaagggt
     4921 ctatctttga catgagctac acgaacctcc gttacatgaa ttgcaggacg agcctgaaga
     4981 atttgctgaa gaagatggcg aggaaaatga agacaccatt tttttggtgt tttcacatcc
     5041 acacttggga cattttgggt ttttggcgtt atacgataaa agctctgaaa atttgtgccc
     5101 acaagaagga cacttgtact catagattgg cattctttgt accttcgatt gattcttcca
     5161 gtattgcctg agattttgtt atggatgttt ttgcctgttc taggattttt ctgacaaaat
     5221 cattggtctg gcaaaccatg ttttttgact cggtttttgc ttcctccatt atcctggagg
     5281 cctcaaaatt tgcctggttg actatctcct gggccttttc agtggcctct tttatgattt
     5341 tggccctctc atcggcgttg agttttattg attcagcttc aatctcggct tgcctgatca
     5401 gatctgcacg ctttcttatg atctccgagg cttcttcaaa ttcgacagga aaatcctgga
     5461 ttattctttc aagaatttct ctcgcctctt tttcaggaat gaggatggtt tttgaaaaag
     5521 ctgccttctt gccattcaag ataagctctc tgaagttttc aagatcagat attatgccca
     5581 ttttcctcca gctcctttag cagttccaga acaacacttg gaacaaacgc tgatatatct
     5641 ccgccctctc tggctatttg cttgacgaaa gttgaggata tgaagctgta ttcaggttct
     5701 gacatcaaaa aaaccgtttc cagttcgggc atgactacat tgttgatggt tgacatccta
     5761 tattcaacat caaaatcgga gattgtcctt aagcccctta caatcacgtt tgaatttgtt
     5821 ttttttgcac aatcaaccag gagcccggta aaaccaataa tttttacatt gagatttccg
     5881 gagaagattt gtttcaaaag atttattctt tgctcgtaca tgaggagagg gtcttgccag
     5941 atattttggc aacagccact gtcacatccc caaacagg
//
LOCUS       DAOSRI010000082         5970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12164_length_5970_cov_3.359594, whole genome shotgun sequence.
ACCESSION   DAOSRI010000082 DAOSRI010000000
VERSION     DAOSRI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12164_length_5970_cov_3.359594"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK717_03455"
     CDS             complement(<1..535)
                     /locus_tag="PK717_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46752.1"
                     /translation="MEEKVVPRKPKYFFKWRYLRYLSQAIFAGSVIFLSWRHLAFATK
                     MAGPIDTYCPFGGFVTLPKLAMTGEFVRRTSDSNVVLLIGLVVAVLVFGGAFCGWACP
                     LGGVFEWLYRLRLKLFKKRIELPRKLHNVLRWFRYVVLALILAMTIIKADLWFKAYDP
                     YSILFTWGHAADIVGIII"
     gene            complement(840..1973)
                     /locus_tag="PK717_03460"
     CDS             complement(840..1973)
                     /locus_tag="PK717_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR46753.1"
                     /translation="MRKGFEMENTDYIKAFDGSISGLFRDATRITLSSPRRAIFFGKM
                     LLAQLRASKVRDKLLREENLRVPPLSIISVTRQCNLHCKGCYAHAQDRIGGEGLTSEK
                     LFDVIAQADDIGMRIAVIVGGEPFTRNDLFRLAQSHKDMVFAVFTNGLLFDDDAIALL
                     KRTPNLVPIISIEGGKEETDRRRGDGTFEKVHEVMRKLKRAGVFYGNSITVTSENTDL
                     VTSGAFVEGLLHLGSRIFFYVEYVPFEAGTQNSVPSDAQRVQLVESVKSFRKRFPGLF
                     IAFPGEENESTGCLAAGKGFVHISARGEVEPCPFAPFSDSSLQNMTLKNALRSEFLRK
                     IRESHGMLDESGGCALFNQRVWLERELGACGKEPCNTKEKVAV"
     gene            2319..2810
                     /locus_tag="PK717_03465"
     CDS             2319..2810
                     /locus_tag="PK717_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOR46754.1"
                     /translation="MMGVSRTTYARILTSACKKVADALTNQKAITLIEGHHNIRKMQE
                     TEKMAKIIAVPVEQDTSNPTVSGHFGKSPFFAVLDLDGEKKEFHSFEHGGCGDIAFFL
                     EKLGVKAVLVKGIGQRPKSVLDQLGIAVLLAQGEDLEQVVENFKKGSNVNFEGSCNHD
                     HGN"
     gene            complement(2820..3419)
                     /locus_tag="PK717_03470"
     CDS             complement(2820..3419)
                     /locus_tag="PK717_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HOR46755.1"
                     /translation="MEQKKVVISPDTKRDKRIPPGQSETTSFPILHYGQMPTVDIEKW
                     RFSIFGLVDEEKSFSYEEFSGLPMTEVRCDIHCVTGWSRLDNVFEGVSALVIRDLVKI
                     RPEAKFVMVHGHNDFTTNIPLHDFFQDDVVFALRFNGEPISPEHGFPVRLVVPRLYFW
                     KSAKWVTGLEFIPRNKLGFWESYGYHEHGDPWKEERYGY"
     gene            3681..3756
                     /locus_tag="PK717_03475"
     tRNA            3681..3756
                     /locus_tag="PK717_03475"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3714..3716,aa:Thr,seq:tgt)
     gene            3874..4419
                     /locus_tag="PK717_03480"
     CDS             3874..4419
                     /locus_tag="PK717_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46756.1"
                     /translation="MPDEKKLAEEAIESEKMLFDIILRLVDTTDKTILDLDSKLNQIF
                     IFYATTMGILIGVVNKDYLTFIFTQAADYTIVFIVFALCFIATLALLINGLWPRKFVE
                     LPDLAWMEKLSDYLKGHPKYTLAELVTHDLEITKKCIFKAIHASERAADLKGFCYKAA
                     LVTIVLSLVAFFTSLVMYYVP"
     gene            complement(4462..5820)
                     /locus_tag="PK717_03485"
     CDS             complement(4462..5820)
                     /locus_tag="PK717_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HOR46757.1"
                     /translation="MPREDQIITAKSDKPIAKCDEDIFGWKDHARMLAKLITDKSNDD
                     VALGISAPWGSGKTSMLNLIKEQIKELKIDPKPIIVEFDPWFYGSEDKVIHSFFETLA
                     KAIFKENHPTREAISKAIGYLSKILTKTPDLPSTFTPNPLVTEAINFAKQFLGTLTDD
                     LKDWIGHGSLNSFRDVKKLLILLLTEAQRKNELPQIIIMIDDLDRLQADEALTMLKII
                     RLVTDLPCIRSIVAFDEKATGQLISNKFNIDGINYIRKMINVPTYLPHISKNDLKQYF
                     AKEMEDILNANGYVYKNISFSQIVDKIGEIVETLRCIKIILNTYKIGISAVGDYVNPI
                     DYLVLCVFFNLTPTLFNVLKKNGVLTSLRISDNNKTGDERIMYVKLNDNVKELMNNYE
                     INDNVLAKTIDDLLSVVFKGLVNGQTTQNYFNRFDSKNICWVDNHKYYFSLLQNLESA
                     DS"
BASE COUNT         1775 a         1334 c         1146 g         1715 t
ORIGIN      
        1 gaattattat tccgacaata tctgcggcat gtccccaggt gaacagaatg ctgtatggat
       61 cgtaagcttt aaaccacaga tcggccttga ttattgtcat tgccagaatg agtgccagaa
      121 caacgtacct gaaccatcta agcacattat ggagcttcct tgggagctcg atgcgttttt
      181 taaacagctt caatctgagc ctataaagcc attcaaacac tccgccaaga ggacaggccc
      241 atccacaaaa agcgccaccg aaaaccagta cggcaacaac aaggccgatc agcaaaacaa
      301 cgttcgaatc gcttgtgcgc ctgacgaact ctccagtcat tgcaagtttt ggaagtgtga
      361 caaaccctcc aaaaggacag taagtatcga ttggccccgc cattttcgtc gcaaaagcaa
      421 ggtgcctcca cgaaagaaaa atcactgagc cagcaaatat cgcctgggaa agatacctta
      481 aatacctcca tttgaaaaaa tactttggtt ttctgggaac aactttttct tccacctttt
      541 cctcctctca ctctctaatg gtagaattat aacaaactag agtcagatat accatccatt
      601 ttattaaaga taaaatacta taccatatat taaaatatca agtataccta tatagggtat
      661 atgaatttat cttctatcca agtcagttca aattgtcaaa cattgctttt ttggaattcc
      721 tgtttcaaat tgatccatcc ccaaataaaa gatttgccag tgtgctcaag gtgagcacac
      781 tggcaaatga gatcactcaa acaacttgtt caaggccaag gtttaccgaa gaattatctt
      841 catacagcaa ccttctcttt tgtgttgcaa ggttctttcc cgcatgcccc aagctctctc
      901 tcaagccata ctctctggtt gaatagcgca caacccccgc tctcatcgag catcccatgt
      961 gattccctga tctttctgag gaattccgag cgaagggcgt tcttcagggt catgttctgc
     1021 agtgacgagt ccgagaaagg cgcgaatgga caaggttcga cttcacccct cgcgcttatg
     1081 tgcacaaaac cttttcctgc agcaagacaa cctgtcgatt cgttttcttc acctgggaag
     1141 gcgataaaaa ggcccgggaa gcgttttctg aatgatttta cgctctcgac aagctggacc
     1201 ctttgggcat cacttggaac actattttgc gtgcctgctt caaaaggaac atactccaca
     1261 taaaagaaaa tccttgatcc aaggtgcagg agtccctcca caaaagcacc ggatgtcaca
     1321 agatcagtat tctctgaagt gactgttatc gagttgccat agaacactcc cgccctcttg
     1381 agcttgcgca tcacttcatg gactttttca aaagtaccat caccccttct tctgtcagtt
     1441 tcctccttgc caccttcaat gcttattatc ggaacaaggt ttggggtgcg tttgagaagg
     1501 gcaattgcat catcatcaaa cagcaggcca tttgtaaaaa cggcaaatac catgtccttg
     1561 tgtgattggg caagcctaaa caggtcattt ctggtaaaag gctcccctcc aacaatgacg
     1621 gcaatcctca tgccaatatc atcggcctgg gcaatgacat caaaaagctt ttcgcttgtg
     1681 agtccctccc cgcctatcct gtcctgtgca tgtgcatagc accccttgca atggaggttg
     1741 cactgtctgg taacactgat tatagaaagc ggtggcaccc tcaggttctc ttccctaagc
     1801 agtttatcgc gcacttttga agccctaagc tgtgccaaaa gcatttttcc aaagaagatt
     1861 gcgcggcgcg gcgatgaaag tgttatcctg gtcgcgtccc tgaatagtcc agaaatcgag
     1921 ccatcaaaag cttttatgta gtcagtgttt tccatttcaa atcctttcct catatccaac
     1981 aagacattag acatgtttgc aagaacttca tttgggaggc cacattaaaa ctcttaagaa
     2041 accaaagaaa tacaggtgaa attgaaatcc tttctgttgt atgggactgg aaatcgatac
     2101 aacaatcaaa aaataaccag tagacacttg agcattatgt gcatatgttc ataatgtaaa
     2161 tatgtccagg ccaaagaatt gccggtttgt tgatagaagt tttatcagaa cacgctttct
     2221 tccagattca aacccaaatg gtgatatcgt tgtgactgcc gatgaggtgg aagctctcaa
     2281 gctttctggc gacaattttc ttcagataaa ggcgtgggat gatgggtgtg tcgaggacca
     2341 cttacgcaag gatactcacc agcgcatgca aaaaagttgc agacgccctt accaaccaaa
     2401 aggcaattac actgatcgaa ggacatcata acataagaaa gatgcaggag acagaaaaaa
     2461 tggcaaaaat tattgcagta ccagttgaac aggacacgtc aaatccaacc gtatcagggc
     2521 attttggcaa atcaccgttt tttgcggtgc ttgatctcga tggcgagaaa aaggagttcc
     2581 actcgtttga acatggtggt tgcggagata tagctttttt tcttgaaaaa ctcggcgtaa
     2641 aagcggtatt ggtcaagggg ataggacaaa gaccaaaatc tgttcttgac cagcttggaa
     2701 tagccgtcct cctggcacaa ggagaggatc ttgaacaggt agtagaaaat ttcaaaaaag
     2761 gctcaaatgt aaatttcgag ggcagctgca accacgacca cgggaactag ttttcaggtt
     2821 cagtacccgt agcgctcctc tttccagggg tcgccgtgtt cgtggtagcc gtaggattcc
     2881 cagaaaccca gcttgttccg cggaataaac tccagaccgg tgacccattt ggccgacttc
     2941 caaaaataaa gcctagggac aaccaggcgg acaggaaaac cgtgttccgg tgaaattggt
     3001 tcgccgttga atctcagcgc aaaaaccaca tcatcctgga aaaaatcatg gagcggaatg
     3061 tttgttgtaa aatcattgtg gccatgaacc ataacaaatt tagcttctgg ccttatcttc
     3121 acgaggtcac ggatcacaag cgcagacaca ccctcgaaaa cgttgtccag ccttgaccag
     3181 ccggtgacgc agtggatgtc gcacctgact tcggtcattg gtagcccaga aaattcctca
     3241 taagaaaatg atttctcctc gtctacaagc ccaaaaattg aaaatctcca tttttcaata
     3301 tcaactgtcg gcatctggcc ataatgtagg attgggaacg aagtagtctc gctttgccct
     3361 gggggaattc ttttgtccct ctttgtgtcc gggcttataa ccaccttttt ttgctccatg
     3421 ggcaggcatg atattccccc aaaaacctcc ggtcaacagg gttggtaaag atattagcaa
     3481 taaatgattt acaagtcatt ccggctcctc aaggccagca ttccataccc agaggtgttt
     3541 tttgtcaatc tttcaggagg cctgcattca aagcaaccca tacttggaag acggtggcgc
     3601 tttgtatacg ttggtctgtc caaacaggca atgttttttc ttgacttgag gcactggcaa
     3661 taataggata tcaagtcgac gccggcgtag ctcagttggt agagcagctg acttgtaatc
     3721 agcaggtcga ccgttcgaat cggttcgccg gctccagtct tttaaaagac acccgtcaaa
     3781 acatccgcaa atattgacta ttaaaaaaac aaacaatatt atttgctagc aaagacattg
     3841 cctggcaaaa agcacgggca aaaggcgggg gccatgccgg acgaaaagaa gctagctgaa
     3901 gaagcaatcg aatccgaaaa gatgcttttt gacataatcc tgaggctcgt ggacacaaca
     3961 gacaaaacca tcctcgactt ggactcaaag ctaaaccaga tcttcatttt ttacgccacc
     4021 acaatgggca tcctgatcgg cgttgtaaac aaggactacc tgactttcat cttcacccag
     4081 gccgccgact acacgatagt tttcatcgtt ttcgcgctct gctttatagc cacccttgcc
     4141 ctgttgataa atggcctttg gccaagaaaa tttgttgaac tgcccgatct ggcatggatg
     4201 gagaaactgt ccgactatct aaaaggccac ccaaagtaca ccctcgccga gttggtaaca
     4261 cacgatctgg agataacaaa aaagtgtata ttcaaggcaa tccacgcaag tgaaagggcc
     4321 gcagacctga aagggttctg ctacaaggcc gcgctggtga cgattgtcct ctcccttgtg
     4381 gcgttcttta cttcgcttgt gatgtattat gtgccgtaag gatagattga aacaacaaat
     4441 atgtctaaga taaatttaag cttatgagtc tgcactttct aaattttgaa ggagtgaaaa
     4501 atagtattta tgattgtcta cccaacagat atttttacta tcaaatctat taaagtaatt
     4561 ctgtgttgtt tgcccattta ctaacccttt gaataccaca cttagtaagt cgtcaattgt
     4621 ttttgctaaa acattatcat taatttcata attattcatt aattctttta cattatcatt
     4681 aagttttaca tacattattc tttcatcacc agttttatta ttatctgata ttcttaatga
     4741 tgtgagtaca ccatttttct ttaatacatt aaataatgtg ggagttaaat tgaaaaagac
     4801 acatagaact aagtaatcta ttggattcac ataatcacca actgcagaaa taccaatttt
     4861 ataagtattt agtataatct ttatacatct aagcgtttca acaatttcac ctattttgtc
     4921 tacaatttga gaaaaagata tgtttttata tacataacca ttagcattta aaatatcttc
     4981 catttccttt gcgaaatatt gtttcaaatc gtttttacta atatggggca aataagtagg
     5041 tacattaatc atttttctaa tataatttat accatcaata ttaaacttat tagatattaa
     5101 ttgcccagtt gctttctcat caaatgcaac tattgatcta atgcatggca aatctgttac
     5161 taaccttatt atttttagca tcgtcaacgc ttcgtctgcc tggagtctgt ccaagtcatc
     5221 aatcataatt attatttgag gcaattcatt ttttctttgc gcttctgtta gcaataaaat
     5281 aagcagcttc ttcacgtcac gaaatgaatt caaagagcca tgtccaatcc agtcttttaa
     5341 gtcatcagtt aaggttccaa gaaattgttt tgcaaaattt attgcttcag taaccaaagg
     5401 gttgggagtg aaagttgagg gtaaatctgg tgtttttgtt aatatctttg aaagataccc
     5461 aattgctttt gatattgcct ctctggttgg atgattttcc ttaaaaatag ctttagctaa
     5521 ggtttcaaaa aagctatgga ttaccttatc ttcagaacca tagaaccatg gatcaaattc
     5581 aacaatgatt ggttttggat caatttttaa ttcttttatt tgttctttta taaggttcaa
     5641 cattgaagtt ttccctgagc cccaaggagc agaaattccc aaagcaacgt catcattgct
     5701 cttatctgta atcagttttg ccaacattct ggcatgatct ttccaaccaa atatatcttc
     5761 atcacatttt gctataggct tatcgctttt ggctgtaatt atttgatctt ctcttggcat
     5821 cttccctcca ggattaaatt atatgaatac tgtcaagcaa aaatcggagg ggttaaatta
     5881 ttcaaaaata ttccgcaaac tttcacaaac ccaataaaaa tccaaaagcc agggcaataa
     5941 aaccctggct tttggttaac aacaaaactt
//
LOCUS       DAOSRI010000083         5968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12170_length_5968_cov_4.050059, whole genome shotgun sequence.
ACCESSION   DAOSRI010000083 DAOSRI010000000
VERSION     DAOSRI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12170_length_5968_cov_4.050059"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PK717_03490"
     CDS             complement(<1..172)
                     /locus_tag="PK717_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011619287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOR46758.1"
                     /translation="MRALYPGTFDPPTLGHISLIERACSLFGDVTVAVAKISGKDPLL
                     MYEQRINLLKQIF"
     gene            complement(144..737)
                     /gene="rsmD"
                     /locus_tag="PK717_03495"
     CDS             complement(144..737)
                     /gene="rsmD"
                     /locus_tag="PK717_03495"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOR46759.1"
                     /translation="MVKKSKGMRLRIVGGTLRGRVMMLPPEFQGRPTKDMVREAFFDI
                     IGDFVIRANFLDLCCGSGATGIEAISRGARHSWLVDRDFVACKVAREAVKTFKVDESV
                     TVEKMDAGSFVSQASANKLFFDIIFCDPPYEITPEKLSKNLEQMPSILALEGILIIEH
                     DEETEYPEGLFGLSRFKEKKYGGTKLTFYSRPVPGDL"
     gene            complement(738..1247)
                     /locus_tag="PK717_03500"
     CDS             complement(738..1247)
                     /locus_tag="PK717_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOR46760.1"
                     /translation="MSPTQEQIDQLYSKLKTEAEEGGYYLNPDVAFTKNLISGLITNQ
                     TRFGYQSCPCRLATGSIDDDLDIVCPCDYRDPDIAEFGACYCALYVNEEISSGKKQAS
                     SIPERRPPKEQRQKQGTPDVQVEKRSTLLPYPIWRCKVCGYLCSRETPPEKCPICKVG
                     KERFEKYTI"
     gene            complement(1244..1525)
                     /locus_tag="PK717_03505"
     CDS             complement(1244..1525)
                     /locus_tag="PK717_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOR46761.1"
                     /translation="MSVTHVVGENKGTIFIYALSTCGWCKKTKEFLKELGLEYSYVDI
                     DLLPESEEDKIVEEMKVWNPTSSFPTIVINNKKAVIGFQPDKIRELLGI"
     gene            complement(1600..2361)
                     /locus_tag="PK717_03510"
     CDS             complement(1600..2361)
                     /locus_tag="PK717_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR46762.1"
                     /translation="MKYTKILVLFLAVVLLGSLVLTACGGSNTTPTDNGTPTDQGTTD
                     QGTTDQNNNGQTDQGTDKTAGEQGDELVVPGMESNVRLNVGDVVPDLKFKLHPVSAGS
                     ESSLYQIMEENNAKALVLDFWAVWCEPCKEELPYLEQLHRQYKDKGLVVLAATIDPFE
                     DEVSIMETLDGTNDNKIKKSWKKLGEFELIDGQAITMNIPVDGDRAMSKALGVTSIPR
                     TFLIDKDHKLVYQHTGFDEGKVNELIEKVKGVMGE"
     gene            complement(2466..3080)
                     /locus_tag="PK717_03515"
     CDS             complement(2466..3080)
                     /locus_tag="PK717_03515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR46763.1"
                     /translation="MPSKKLNVVFAAMILVLTFSLGCGSGGGGNGGGTGEKKIPDLKY
                     KLHPVSLGNENTIYQVMQENNAKALILDFWAVWCEPCKEELPYLELIHREYKNKGVVV
                     LAATIDPFEDESAIDETLDGTNDSKIKKSWKKLGEFELIDGRVITMEIPVDGDRKIAN
                     ALGVTSIPRTFLVDKDHNIIYQHTGFDEGKVEELKKKLDELLSK"
     gene            3232..3708
                     /locus_tag="PK717_03520"
     CDS             3232..3708
                     /locus_tag="PK717_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOR46764.1"
                     /translation="MGERLTNLVISENELREIDEYLAKTLIDSQAKHIFLVDKSGQLI
                     SKHGSATRQAASAIAALSAGVYAATNELARQIGENEFTITFHQGSENNIHISLLTQET
                     LLIMIFDNRAPIGAIRFWAKKISTALTPVIERMSQAIPTEFGGGDLDKDLDLDNIL"
     gene            3720..4301
                     /locus_tag="PK717_03525"
     CDS             3720..4301
                     /locus_tag="PK717_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HOR46765.1"
                     /translation="MALVNFATKEINVKLVYYGPALSGKTTNLQVIYDQVPSEVKGQF
                     TSIATATERTLFFDYLPLDLGTVRGFKLRLGVYTVPGQAIYGLTRKSILKSVDAIVFV
                     ADSDPDKLEANIENFQDMVENLREYGLDLDKVPLIIQYNKRDLPNAIPFEVLEEALNP
                     EGRFPIYEAVATKGIGVKETLKHVTKLAVSRIH"
     gene            complement(4309..5022)
                     /locus_tag="PK717_03530"
     CDS             complement(4309..5022)
                     /locus_tag="PK717_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOR46766.1"
                     /translation="MDKNICEDFIKGTSYFSGERIDPPELHEELPPSPNIISEPVALP
                     SFDQDTNLLQIINHRRSKRNFENKPLSLVQLSKLCWGTQGETGRVGQTILRAVASAGN
                     RHPFNTYILINKVEELLPGLYFYNNSNSLGPLRLGELGGELSEACLGQKMIESCSVVF
                     IWTAATARTTSVYRARGYRYIFLDAGHIGAQLQLLCVDLGLGSCNIGAFWDDKVAKFL
                     GVKHKYEVPIYITAVGWPK"
     gene            5143..>5968
                     /locus_tag="PK717_03535"
     CDS             5143..>5968
                     /locus_tag="PK717_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46767.1"
                     /translation="MKTIRTIISLALVLGMLAFPGFAKESSKALAASSLIVTPTPSIL
                     TAGMAAELVDPARPFQLMVTDQNGKPVDLTRGVDGSTIDQKVVWNQLFLDPHPDNTYY
                     YGRNAVLPSYYWTRTDLHNDDGTNVCNKLLFGRNLIEIDFSQASRGIYIFKGFVANDA
                     GELRIKVITPDRRLAGEARIAVKAPVVTYKIINTEDTESKAFESPGDPDFVMTACDNR
                     IYNIGVTVQDAEGRVVRGVSQGVSLCTGIKKTARFTPFTTRPANYKWVTKPTLVQGY"
BASE COUNT         1544 a         1380 c         1279 g         1765 t
ORIGIN      
        1 agaagatttg tttcaaaaga tttattcttt gctcgtacat gaggagaggg tctttgccag
       61 atattttggc aacagccact gtcacatccc caaacaggga acacgccctt tcaatcagtg
      121 atatgtggcc cagtgtgggt gggtcaaagg tccccgggta cagggcgcga atagaacgtg
      181 agttttgttc caccatactt tttctccttg aacctggaga ggccaaaaag cccttccgga
      241 tattcggttt cttcgtcatg ctcgatgatc aatattcctt ctagagcaag gattgaaggc
      301 atctgttcca gattttttga tagcttctcc ggtgtaattt catagggtgg atcacagaaa
      361 attatgtcaa aaaaaagctt atttgctgat gcttgcgaga caaaagagcc agcatccatc
      421 ttttcaacgg tcacgctttc atccacctta aaggtcttca ctgcttctct ggcgaccttg
      481 caggcaacaa aatccctgtc gacaagccat gaatgacgcg cacctcttga tatagcttcg
      541 attccagttg cccctgatcc acagcagaga tcaagaaaat ttgctcttat aacaaaatct
      601 ccaataatgt caaaaaaggc ttccctgacc atgtcttttg ttggtctgcc ctgaaattcg
      661 ggcggcaaca tcatcaccct tcccctcagg gtgccaccaa caatccttaa acgcattccc
      721 ttgctttttt tcaccatcta gattgtgtac ttttcgaacc tttcttttcc taccttgcag
      781 atcggacact tttcaggcgg tgtttccctt gagcagagat agccacatac cttgcagcgc
      841 cagattggat atggcaataa ggttgacctt ttttcaacct ggacatctgg ggttccttgt
      901 ttttgacgct gttcttttgg tggtcttctt tcagggattg aactggcctg cttttttcca
      961 gagcttattt cctcatttac ataaagagcg caataacagg cgccaaattc tgcaatgtcg
     1021 gggtctctgt aatcacatgg acacactatg tccaggtcgt catcaatcga gcctgttgct
     1081 agcctgcagg ggcaggattg gtagccaaac cttgtttgat ttgttatgag cccactgatg
     1141 agattttttg tgaatgcgac atcagggttt aagtagtagc cgccctcttc tgcctctgtt
     1201 ttcagttttg aataaagctg gtcgatctgt tcctgagtgg gactcatatt cccaagagct
     1261 cccttatttt atcaggctgg aagccaatga ccgctttttt attgttaatc acaattgttg
     1321 gaaatgagga ggttgggttc cagactttca tctcttcgac tattttgtct tcttcgcttt
     1381 ccggcaggag gtctatatca acataactgt actccaggcc aagttcttta aggaattctt
     1441 tggttttttt gcaccagccg catgtactaa gcgcataaat gaaaatggtc cctttgtttt
     1501 ctcctacgac atgagttacg ctcattccta cacctccttt tttgccatta tacaaaaaaa
     1561 gcgccccttt taaaaggagc gctttgattt tctggagttt tactctccca tcactccctt
     1621 gactttttcg atgagctcgt tgaccttgcc ctcgtcaaag ccggtgtgct gataaacaag
     1681 cttgtggtcc ttgtcgatga ggaatgttct cggtattgag gtaactccaa gtgctttgga
     1741 cattgccctg tcgccatcga ccgggatgtt cattgtgatt gcctgtccat cgatgagttc
     1801 gaactcgcca agcttcttcc atgatttctt gatcttgttg tcgttggttc catcaagtgt
     1861 ttccatgatt gaaacttcat cttcaaatgg atcgatggtt gcagcaagaa ctacgagacc
     1921 cttatctttg tactgcctgt ggagctgctc aagatacggg agctcttcct tgcatggttc
     1981 gcaccaaact gcccagaaat caaggacgag tgcttttgca ttgttctctt ccatgatctg
     2041 gtagagggac gattcggaac ctgcagaaac tgggtggagc ttgaacttga ggtccggaac
     2101 aacgtcgcca acgttcagtc tgacattgga ttccattcct ggaactacca attcatcacc
     2161 ctgttctccg gccgtcttat ctgtgccctg gtcggtctgt ccattattgt tttgatcagt
     2221 tgttccctgg tcggtagttc cctgatcagt tggcgttccg ttgtctgtcg gagttgtatt
     2281 gcttccgccg catgctgtca gaactaatga accgagaaga acgactgcca agaaaagaac
     2341 caatattttt gtgtacttca ttttgcctcc tttaaggatg acccttttgt acatatactt
     2401 acacacgagt caaccacaaa atcgtgtcaa aagtatttaa ggccggttta atttgactta
     2461 atcaattatt tggaaagcag ttcatccagt tttttcttca attcttcgac ttttccctca
     2521 tcaaatccag tatgttgata gattatatta tgatccttgt cgacgagaaa tgtccttggg
     2581 attgatgtta cacccagtgc gttggcgatt ttcctgtctc catcaaccgg gatctccatg
     2641 gttatgaccc ttccgtcgat taattcaaat tcgccaagtt ttttccatga ttttttgatt
     2701 ttggaatcat ttgttccatc aagcgtctca tcaatcgccg attcatcctc gaaaggatca
     2761 attgttgccg caagcacgac aacacccttg tttttgtact ccctgtggat gagttccagg
     2821 tatgggagct cttccttgca tggttcgcac cagactgccc aaaagtctaa gatgagtgct
     2881 tttgcattat tttcctgcat tacctgatat atggtgtttt cgttgccaag cgaaaccgga
     2941 tgtagtttat attttagatc agggattttt ttctcgcctg tccctcctcc atttccacca
     3001 cctccactcc cacagcccaa tgaaaaggtc aggaccaaaa tcatcgctgc aaaaacgacg
     3061 ttaagttttt ttgaaggcat tttacccctt tccaaaagca aaattatcct atatcgcttc
     3121 attagtcaac ggtggtgtaa tttcttataa atgttgaatt ttatggcttt ctcgttgaca
     3181 caaagacgat tgtttggtaa gttaacccca gttcaaataa ggagatgttc aatgggcgaa
     3241 aggcttacca accttgtcat ttctgaaaac gaattgaggg agatagacga gtatctggct
     3301 aaaactctca tagattctca ggcaaaacat atttttcttg tagacaaatc tggccagctc
     3361 atctcgaagc atggatcggc cacccgtcag gcggcttcgg ccattgctgc gctgtctgct
     3421 ggtgtttatg ctgcaaccaa cgaactcgcc agacaaattg gcgagaatga atttacaata
     3481 acattccacc agggttctga gaacaacata cacatatcac tcctcactca ggaaaccctg
     3541 ctcattatga tctttgataa cagggcacca ataggtgcca tcaggttctg ggcaaagaag
     3601 atttcaacag cgctcacacc agtaatagag agaatgtcgc aggccatacc gacagaattt
     3661 ggtggcgggg atctggacaa ggacctcgat ctggacaata tcctttgagc tggtgacaaa
     3721 tggccttagt taattttgca acaaaagaaa tcaatgtaaa acttgtttat tatggcccag
     3781 ccctgtctgg aaaaacgacc aacctgcagg ttatttatga ccaggttcct tcagaggtca
     3841 aaggccagtt cacttccatt gcaacagcca ctgagaggac cctatttttt gattatctgc
     3901 cgcttgacct cgggactgta agaggtttta agctcaggct tggtgtctac accgtccctg
     3961 gccaggcgat ctatggtctt actagaaaat caatcctgaa gtctgttgat gcgatcgtct
     4021 ttgtggcaga ttctgaccct gacaaactcg aagcaaacat agagaatttt caggatatgg
     4081 ttgaaaatct cagggaatat ggccttgatc tggacaaagt tcccctcatc attcagtaca
     4141 acaagcgtga tcttccaaat gcgataccgt tcgaggtgct cgaagaggca ctcaatccag
     4201 agggaaggtt ccctatttat gaagccgttg caacaaaagg cattggtgtg aaggaaaccc
     4261 ttaagcacgt caccaagctg gctgtctcaa gaatacatta aaaacctttt atttcggcca
     4321 tccaacagca gttatgtaga ttgggacttc atatttatgt tttactccta ggaattttgc
     4381 cactttgtca tcccagaaag cacctatatt gcaggatcca agtccaagat caacacaaag
     4441 gagttgcaat tgtgccccga tgtgtcctgc atcaaggaag atatacctgt aaccccttgc
     4501 cctgtatact gaagttgttc ttgctgttgc ggcagtccat ataaaaacta ccgaacagga
     4561 ctctatcatt ttttgtccca gacaggcctc agacagctcg cctccgagtt caccaagcct
     4621 cagtggccca agagagttgc tattattata aaaataaaga ccaggcaaaa gctcttccac
     4681 cttgtttatt aagatatatg tgttgaatgg gtgtctgttt ccagcagaag ccactgccct
     4741 cagtatagtc tggccaactc tgccggtctc accttgtgtc ccccagcaaa gttttgataa
     4801 ctgaacaagt gaaagtggtt tgttttcaaa atttcttttt gatctcctgt ggtttataat
     4861 ttggagcaga ttggtgtcct ggtcaaaact gggcaatgca actggttcgg aaataatgtt
     4921 gggtgatggt gggagctctt catgaagttc tggtggatca attctttcac ctgaaaaata
     4981 cgatgttcct tttataaagt cttcgcaaat attcttatcc aaattgcacc tccaccaaaa
     5041 atattgcacc aaattatcat tttggcaaca gtggcgcgtc atgggttgaa cttttaaggg
     5101 atgttttcgg tattgagttt gaaatcagcc aggaggtttt gaatgaaaac cattagaaca
     5161 ataatttcgc tagccttggt ccttgggatg ttggcattcc cgggatttgc gaaggaaagt
     5221 agcaaggcac tggcagcgtc ttccctcatc gtgaccccaa caccatccat cctgacggcc
     5281 ggaatggccg ccgaactagt tgatccggca aggcctttcc agctcatggt cacggaccag
     5341 aacggaaagc ctgttgatct gacaagagga gttgatggtt ccactattga ccaaaaagtc
     5401 gtatggaacc agttatttct ggaccctcac ccggacaata catattacta tggcagaaat
     5461 gcagttcttc ccagctacta ctggacgaga actgacttgc acaatgatga tggaacaaat
     5521 gtctgcaata agcttctttt tggaaggaac cttattgaaa tcgatttttc acaggcttcc
     5581 aggggaatat acatcttcaa aggttttgtg gcaaatgatg ctggcgagtt gaggataaaa
     5641 gtcataacgc ccgacagaag gcttgcaggg gaagcaagga tagccgtaaa ggcacctgta
     5701 gtcacctata aaatcatcaa cactgaagat acagaatcaa aggcttttga aagtccgggc
     5761 gacccggatt ttgtcatgac cgcatgtgac aacaggatat acaatattgg ggtgactgtt
     5821 caggacgcag aaggaagagt agtcaggggt gtctcgcagg gagtcagcct ttgcactgga
     5881 atcaaaaaaa ctgctaggtt tacaccattt actaccagac cggcaaacta caagtgggta
     5941 accaaaccaa cacttgttca gggataca
//
LOCUS       DAOSRI010000084         5956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12199_length_5956_cov_4.105406, whole genome shotgun sequence.
ACCESSION   DAOSRI010000084 DAOSRI010000000
VERSION     DAOSRI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12199_length_5956_cov_4.105406"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1143
                     /gene="miaA"
                     /locus_tag="PK717_03540"
     CDS             232..1143
                     /gene="miaA"
                     /locus_tag="PK717_03540"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR46768.1"
                     /translation="MKKVAVIFGPTASGKTDLAIKLAKEFGGEVVSCDSQQIYRELFI
                     GTARPTEKEMDGIVHHLLGHVSVREKYNVARYLDDVSIAINEVLSRGKLPIVCGGSYM
                     WLMMMIDGISKTPEGDPDLRRKLENEAKEDGLAAIYARLQTVDPVSASKIKPTDLKRI
                     VRALEIYELSGKTQSELFSEREPLLYDFIKIAITRPMEELYGRINRRTDEMVSAGLLD
                     EIKTVIENGLEADVLRVMSHGYPFLIDYFHGRTTLEKALSDMKLVTRHYAKRQMTFIR
                     SRQDFNVFDARDFEGICSFVHTKLLDK"
     gene            1188..2144
                     /locus_tag="PK717_03545"
     CDS             1188..2144
                     /locus_tag="PK717_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46769.1"
                     /translation="MDPTRNERHQAIERIEAERNSKVISYILHPEGASIADDVVPPLY
                     HQLMTMGRTDRLDVILASRGGSPESAWRILTLLREFTDHLSVIVGFKAFSAAAFIALG
                     ADEIVMSPLSELGPVHIQTASPLAPIGADGNPAWVSPYDIMTYVEYAKTNGANPEMIF
                     GDIDLHPLVLGSAMRAYNLAKMVATKCLGLSSKARGQGEMDRIVDAFIGGTHSSSMPF
                     TRFDCLEELKLPVTYASPQLEASIGMLVSVYNQSVSYSSEPETDPISKREYRTITPAI
                     LESLGLTQVYKRRIERIVTPEGNVTEVPGWGGWMNETVQEAM"
     gene            complement(2213..3619)
                     /locus_tag="PK717_03550"
     CDS             complement(2213..3619)
                     /locus_tag="PK717_03550"
                     /inference="COORDINATES: protein motif:HMM:NF023045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HOR46770.1"
                     /translation="MKKLLAVLLTILMAVPLFAFSPVLFAKDGDKGGGNGGKEEKCNF
                     TARFEKEPATIAMEAGQEETFTLLVTNNCGMEQLKIKASSENTGLELGNAEFEVAPKG
                     TFRLTVKIKQPPKSGTSNEVHWMIKLVSQYGKQLEFKFITRYKITQECRYSVKWEKDP
                     TSGKLDPGQEGEYNLVVTNLGTSKITFKVTSKTEGLRFSVNEFSAEAGQKFILKVIIK
                     QTAKGQENFSKWAFVIATDCGGQKEIVFRVFFKVTEQPSCKFTFRWQKSPESVKLDPG
                     QEAEFPLEITNKGTQKTTIKIEYSGEFLKFSAKEFELEPGAKFVLKVTVKQPPIGKEP
                     ATKFSFVLVASCGQKAQISFRIFYKTPPASNCEFEVRWEKNPANTPLKAGQRESFNLL
                     IKNKTGKNMKFAASSGSSAIKFSPDRLEVPNGHEKKMKVTITMPGKQNDEKEKTFEIT
                     IKADCGTTKVVKFTVKYK"
     gene            3737..4072
                     /locus_tag="PK717_03555"
     CDS             3737..4072
                     /locus_tag="PK717_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOR46771.1"
                     /translation="MESDICFEEKFTKEFFLGFIKLHVLHHSTKEPVYGLWFIEELSR
                     HGYKMSPGTLYPILHQLEKNGYLESFREKVAGRLRVYYEATENGKKALEEARSKIKHL
                     IKEVLYDEE"
     gene            4059..>5956
                     /locus_tag="PK717_03560"
     CDS             4059..>5956
                     /locus_tag="PK717_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR46772.1"
                     /translation="MTRNDIGLTSAKAKELLAKFGPNRIFKPYTVNFIGIFIEEVTEP
                     MILLLIAVGIIYSFLGELGDSITIFSIIVLLTLAEVFNEYRAKKAVASLGEIATPKSR
                     VIRDGNVAEVDSLEVVPGDVLALSQGTKVAADAKIIRSVGLQVDESALTGESFPQDRD
                     AGESIFAGTAVVFGEGEAIVEKTGASTRLGEIAAKTKAVKPPKTALQRAMKSLSGQLV
                     WVALFFSIMIPLVGFLRGGDWKAMILTGLTLSFATIPEELPIIITMVLGIGSYRLSKK
                     NFLIKKLKAAETLGNTTVIVTDKTGTITQAKMSVAATFPKDAKESLSLALLSTTDYSS
                     NPLETSIKQKALELGASPASPEIFRQRSVGDGKKSKSVIRKNDSIYTLYKSGAPEDVL
                     ASCASVPKEVSGELEKQAENGRRVIAVATKNLTQGEAKEDFDKLEKQMTFESLIAFED
                     PPRDGVRETIIRAKGAGIRTIMVTGDHPKTAAFIANQVGIDTANIVTGDKLDLISDSE
                     LKETVRTMSVFARTVPEHKYRIVKALQENSEVVAVTGDGINDVLALKGADIGIAMGKR
                     GTDVARDAAEVVLSDDNYNTIASGIFEGRAFFDNLQKGLKYYLAVKIALVLTFLPPVI
                     LGVPMPFA"
BASE COUNT         1568 a         1397 c         1489 g         1502 t
ORIGIN      
        1 aatccttggc ttctcttcct caagtgtcgg ccccaccggt ttgtctccaa aaatccatac
       61 tgccaccagg atgcctgcaa ttagcatcag agcggccacc acaatagcta ccggaactgg
      121 ttttcttgtc atttgcgcct ccttgaatac caaacaggcc agatacccct gttgttcaat
      181 atgatattta atcccctttg ccttatttgg caattctgtt ataaggaaag tgtgaagaaa
      241 gttgctgtaa tatttggccc tactgcaagc gggaagaccg atcttgccat aaagctggca
      301 aaagagtttg gaggagaagt tgtttcttgc gattcacagc aaatataccg cgagcttttc
      361 attggcactg ccaggccgac agaaaaggag atggacggaa ttgtccacca ccttctgggg
      421 catgtcagcg taagggaaaa gtacaatgtt gccagatacc ttgatgatgt gagcattgca
      481 ataaacgagg ttctctcaag aggaaaattg ccgatagttt gcggcggttc ctatatgtgg
      541 ctaatgatga tgattgatgg tatttcgaaa accccggagg gtgatccaga tttacgcaga
      601 aaacttgaaa atgaggccaa agaggacgga ctggcggcca tatacgccag actccagaca
      661 gttgacccgg tctcggcgtc aaagataaaa ccgactgatt tgaaaaggat tgtaagggca
      721 ctggagattt acgagctttc cggcaagacc cagtcggagc ttttttctga aagagagccc
      781 cttctttacg attttataaa gattgccatc acaaggccaa tggaagaact ctacgggaga
      841 ataaacaggc gcaccgatga aatggtcagc gccggcctgc ttgacgagat aaaaacagtg
      901 attgaaaatg gccttgaagc tgatgtcctc agggtgatgt cccatgggta ccctttcctg
      961 attgattact ttcatggcag gacaacgctt gaaaaggcac tctcggacat gaaacttgtg
     1021 accaggcact atgcaaagag gcagatgact ttcatcaggt caaggcagga ttttaatgtt
     1081 tttgatgcaa gagattttga ggggatttgt tcttttgtcc atacaaagct tcttgacaaa
     1141 tgacagcgac cagagacatt taacagggca aaaaggagga cacaaaaatg gaccctacaa
     1201 ggaacgaacg ccaccaggcg atagaaagga tcgaggccga gaggaattcg aaagtcatat
     1261 cctacattct ccatccggag ggcgcctcga ttgctgatga tgtcgtccct ccgctctacc
     1321 accagctcat gacgatgggc aggacagaca gactcgatgt gatactcgca tccaggggcg
     1381 gttcaccaga gagcgcctgg agaattctga cccttttgag agagtttaca gaccacctgt
     1441 cggttattgt tggcttcaag gccttcagcg cggcggcatt cattgccctt ggtgccgacg
     1501 aaattgtcat gtcgccactc tccgagctag gccccgtaca catccagaca gcaagcccgc
     1561 ttgcgccgat tggcgctgat gggaacccgg catgggtcag cccctatgac atcatgactt
     1621 atgttgaata cgcaaaaaca aatggcgcca acccggagat gatattcggt gatatcgacc
     1681 ttcatccact tgtgcttggt tctgcaatga gagcgtacaa tctcgccaaa atggttgcca
     1741 caaagtgcct cggcctttcc agcaaagcga gggggcaggg tgaaatggac agaatagttg
     1801 acgcatttat tggcggcacg cactccagca gcatgccatt tacaaggttt gattgccttg
     1861 aggagctgaa actgcctgta acctatgcct cgccacagct tgaggctagc attggcatgc
     1921 tggtttcagt ttacaaccag agcgtgtcct acagctcgga gccggagacc gatccgattt
     1981 caaagaggga gtacaggaca ataacgccgg caatcctgga gagccttggc ctgacccagg
     2041 tctacaagag aaggatcgaa aggatcgtca caccagaagg caatgtcacc gaggtgcctg
     2101 gctggggtgg gtggatgaat gaaaccgtcc aggaggcgat gtaaaaaaat tcatttctga
     2161 taaataaaaa aagcccgagg aaacccgggc tttttttata tggagagagc gtttatttgt
     2221 attttacggt aaatttgacc acctttgtcg tgccacaatc tgcctttatt gttatttcga
     2281 atgttttttc cttctcgtca ttctgtttac caggcattgt tattgtgacc ttcatctttt
     2341 tctcgtggcc gtttgggact tccagcctgt ccgggctgaa ctttattgca gaggagcctg
     2401 aggaagcagc aaatttcatg ttcttgcccg ttttgttctt gatgagcagg ttaaatgatt
     2461 ctctctgacc ggccttgagg ggtgtatttg ccgggttctt ctcccatctt acttcaaatt
     2521 cgcagttact tgctggtggt gtcttgtaga aaatcctgaa ggaaatctgg gctttctgtc
     2581 cgcatgaggc aacaagcaca aacgagaact ttgtggcagg ttctttgccg atcggcggct
     2641 gcttcactgt cactttcagc acaaactttg cacccggttc cagctcgaac tccttggccg
     2701 aaaacttgag gaactcaccg gagtattcaa tcttgattgt tgttttttgt gtgcccttgt
     2761 ttgtgatctc aagcgggaat tcggcctcct ggccagggtc aagctttacg gattccgggg
     2821 acttttgcca tctgaaggtg aacttgcatg atggttgttc cgttacctta aagaatactc
     2881 tgaatacaat ttctttttgc ccgccgcaat cagttgcaat cacaaaggcc catttcgaga
     2941 aattctcctg gccttttgcg gtctgtttta ttatcacttt caggataaac ttttgcccgg
     3001 cctcggcaga gaattcattt accgagaagc gaagcccttc ggttttcgag gtcaccttga
     3061 aggttatttt ggaagttcca agatttgtga ccacaagatt gtattcaccc tcttgccctg
     3121 gatcgagttt gcctgatgtt gggtccttct cccacttgac gctatacctg cactcctggg
     3181 tgatcttgta tcttgttata aacttgaatt ccagctgttt tccatattgt gaaacaagct
     3241 ttatcatcca gtggacttca ttagaagttc cagattttgg tggttgcttg atcttgactg
     3301 tcagcctgaa ggtgcccttt ggtgcgacct cgaactcagc attgccgagt tccagtccag
     3361 tgttctccga agaagccttt atcttgagct gctccatgcc gcaattgttt gtgaccagca
     3421 aagtaaaagt ctcttcctgg ccggcttcca ttgcaatcgt tgcgggctct ttttcaaacc
     3481 ttgccgtaaa gttgcatttt tcttcttttc caccattgcc gccgcccttg tcgccatctt
     3541 ttgcaaacaa aacaggcgag aaagcaaaca atgggactgc catgaggatg gtcagtaaaa
     3601 ccgccaacag tttcttcatt tgaaacctcc ttggtctttt ctgaaatcta atacggtgca
     3661 tgtatttaaa aagtttacac tttaatgaaa gcctctcttg aaaacggaaa attgaatatt
     3721 atatcgaaat ccgataatgg aaagcgatat atgttttgag gaaaaattta ccaaggaatt
     3781 ttttcttggg tttatcaaac tgcatgtatt gcaccactcg acgaaagagc cggtatatgg
     3841 gctttggttt atagaagaac tttcacgcca cggctacaag atgagccctg gtacgcttta
     3901 ccccattctc caccagcttg aaaagaatgg ctatcttgaa tcattcaggg aaaaggttgc
     3961 tggaaggctg agggtctact acgaggccac agaaaatggc aagaaagctc ttgaagaggc
     4021 aaggagcaag ataaagcatc ttataaagga ggttctctat gacgaggaat gatattggac
     4081 tgacttctgc aaaagcaaag gagcttcttg caaaatttgg gcccaacagg attttcaaac
     4141 cctacacggt aaactttatt ggcattttca tcgaagaagt tacagaacca atgattcttt
     4201 tgctaatcgc tgtcggtatc atatacagtt ttcttggtga acttggcgat tcgataacga
     4261 tattttcaat tatagtcctc ctcacgcttg ccgaggtgtt caacgaatac agggccaaaa
     4321 aggccgttgc aagccttggt gagattgcta ccccaaaaag cagggtgata agagacggca
     4381 atgttgcgga ggttgactcg cttgaggtgg tgcctggcga tgttcttgcc ctctctcagg
     4441 ggacaaaggt cgctgccgat gcaaaaataa tcaggtcagt tgggctccag gtggatgaat
     4501 cggcactcac tggagagtcg ttcccacagg acagggacgc tggcgagtcc atcttcgccg
     4561 ggaccgccgt tgtctttggc gagggcgagg caatagtcga aaaaactggc gcaagcacaa
     4621 ggcttggcga gatagcggca aaaacaaagg ccgtaaagcc accaaagacc gccctccagc
     4681 gcgcgatgaa atcgctctcc ggccagcttg tgtgggtggc acttttcttc tcgataatga
     4741 taccgcttgt cggatttctc aggggcggag actggaaggc catgatactg accggtttga
     4801 cgctttcttt tgccacaata ccagaggaac tgccaatcat catcacaatg gtgctcggga
     4861 ttggctccta caggctgtcc aaaaagaact tcctgataaa aaagctcaag gcggctgaaa
     4921 cacttggcaa cacaacagtc attgtcaccg acaagacggg aacaatcacg caggcaaaga
     4981 tgtctgttgc tgccactttc ccgaaagacg caaaagaatc cctgtctctt gcgctccttt
     5041 cgaccactga ttattcaagc aacccgctgg agacatcaat aaagcagaag gcgctggaac
     5101 tcggtgcatc gccagcatcg ccggaaattt tcagacagag gtcggttggt gatggcaaaa
     5161 agtccaaatc tgtgatcaga aaaaatgact cgatttatac gctctacaag agtggggcgc
     5221 cggaggacgt gcttgcaagc tgcgccagtg tccccaaaga agtctccggg gagcttgaaa
     5281 agcaggcaga gaatggcaga agggtcattg ctgtcgccac aaaaaacctc acccagggtg
     5341 aggcgaagga agattttgac aagctcgaaa aacagatgac atttgaatca ctgatcgctt
     5401 ttgaagaccc gccaagggac ggcgtcaggg aaacgataat aagggcaaaa ggtgccggaa
     5461 tcaggaccat aatggtcacg ggagaccacc cgaagacggc cgcattcata gcaaaccagg
     5521 ttggaatcga cactgcaaat attgtgactg gcgacaagct tgacctgata agcgattcag
     5581 agctgaaaga aaccgtcagg acgatgtctg tttttgccag gacagtcccg gagcacaaat
     5641 acaggattgt aaaggcgctc caggaaaaca gcgaggtcgt ggccgtgacc ggcgacggaa
     5701 tcaacgatgt tctggcgctc aagggtgcag acatcggcat cgcaatgggc aaaaggggaa
     5761 ccgatgttgc cagggacgcg gccgaggtcg tgctctccga cgacaattac aacacaattg
     5821 caagcggaat ctttgagggc agggcgtttt ttgacaacct gcaaaagggc ctcaaatact
     5881 accttgcggt caaaatagcc ctcgtgctga cgttcctccc gcctgtaatc cttggcgtgc
     5941 cgatgccgtt tgccca
//
LOCUS       DAOSRI010000085         5939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12245_length_5939_cov_3.640891, whole genome shotgun sequence.
ACCESSION   DAOSRI010000085 DAOSRI010000000
VERSION     DAOSRI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12245_length_5939_cov_3.640891"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="PK717_03565"
     CDS             <1..1938
                     /locus_tag="PK717_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR46773.1"
                     /translation="IVNPPKQDISGVKIISPINGQIINQGDFFEAKAEIPLKGQGTYE
                     ISGKWIVDGVSHPFSQKMQVAWQAVAKVSQVLPTNVSGDHTVELIVDLPTAYKSGIIK
                     YTVSLKAPIAVVLDPVIQDATVSIDGTTKIKIKINVTQAGSFNLLGYLLLDGKVYDKL
                     EKIVIGPGEFIYEMTIPAQSAGPHTVQFKLVSPTELLSNAVTFRVVGEAWVWPGIIYP
                     GDGTTVTQNSPLVAELGIKVGGKGTIKVVGVWMIDDLPYRDYEQMLQITSETIILEKM
                     PLPTDAPGWHRLKFRMTWPYYEETKEIWYRIWGREQPPSFLQVITHPQSPYSNYQSFQ
                     LQVRAQDDRGIKNLCAYVDFAKHVDVSMGGMMVIDYITPTIGPLPVGEHTWSVTLTDL
                     DGLESEYIGKFVVTKSSGVLEGFVLNKITNGPIVDAEVSCAGHLVKTDAYGKYRIDGL
                     GEGEQVVMATHQSYRSAEARVTIFAGVTTNAPTLYLDDKGPLPFVYQIEHWPQVPKTG
                     ESFVLSVYVRNDGADADLSEVAISCPEGAVIEIDPDTGAQYPSFAYVYYPGSFIDHRD
                     GQQIRAIHPVAIVRWNSWGSGVTRKVMIKVSPQEPKPFNFWVRAMMSKGLSKEIFPRN
                     SEIIDQQGYPIKEYRVEVQPK"
     gene            1938..>2257
                     /locus_tag="PK717_03570"
     CDS             1938..>2257
                     /locus_tag="PK717_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46774.1"
                     /translation="MKKSLLLLIVFSVLFASPAIAEPPTDPGNSGRVNFIGREPMTMR
                     WKKHVDYETEELPCATNGKHVLVVSGQYSIVCLDADTGNQLWKRQTVDYVKYTPCVTS
                     TMGLV"
     assembly_gap    2258..2267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2268..4106
                     /locus_tag="PK717_03575"
     CDS             <2268..4106
                     /locus_tag="PK717_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46775.1"
                     /translation="VTSKLGYIQAFDLERGEQKFLINLGFDELTTPVIGENYVFLKGL
                     NHVARTSSAMAIDLYKGRVVGSYVQCDYSLEPPVLSQKEVFVVTPNRKGIQAISQSTL
                     LTIWTKNLNDEVTHISTRMNHVVAVCRSGNVYTMDNATGNIVWKTNVTGPVIWPATIS
                     IDHVIICSGSNVIYSFRLSDGTQLWEKRWPVTVQPQSSRAYDIFCFGKMMSFHDPKSG
                     EKLWQLELPSETIGQPIPFLDQIFAATKSNKIVALKTAGFEISLSSKKLDLGSVSSSH
                     PYTTSQLTVRNNSGEIQQIFTSSSNPWISVSHNGFEIMPFDQIDIAIELDMHSLTLGQ
                     SQGEIVVAWKNGSIKVDVMAKKIGDKEAVPPKPGFLSLGTTEATLEGRLNMGVPLASI
                     TLFNKGELPVSYIAKSEVPWVVIGRPNGRIYGNTSTYFTATIINDLASVGENTAEIYA
                     TCPETGQCFTIPLKYTRSLGRTHVIFQMRIGSSVGHLGQSKIRVRPEPFIENNSIMVP
                     LEIFKLALDATLDVYEYTYEPTYYVLQRGDIKITHRLGESKVYVQEGAGEINEILLPC
                     PSRLINERPVVPLLGIAMALKGEMIYSQADGKVTLDVFLPELPTQK"
     gene            complement(4063..>5939)
                     /gene="uvrA"
                     /locus_tag="PK717_03580"
     CDS             complement(4063..>5939)
                     /gene="uvrA"
                     /locus_tag="PK717_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR46776.1"
                     /translation="IQRRHKQTQSDGMRQAYEELMIEVPCPVCGGKRLKPEALSVKVS
                     GKNIAEIASWPIDKAFNFFEMLKLSEKNMAISKQVVKEIKSRLNFLLDVGLAYLSLDR
                     GSATLSGGEAQRLRLATQIGSSLSGVLYVLDEPSIGLHSKDNSKLLSALKRLRDLGNT
                     LIVVEHDEETIREADFIVDIGPGAGVNGGHVVGTGSVSEICKVPISETGMYLSGKKKV
                     EIPDERRSGDGRSIKIVNPTEHNLKGNDITIPLGTLTVVTGVSGSGKSTLVNDILYAS
                     LAKEFYASREMPGKCEKIEGIRNLDKVIIVDQSPIGRTPRSNPATYTGLFTYLRELFA
                     KLPESKARGYEAGRFSFNVKGGRCEACEGDGMIKIEMQFLPDVYVPCEVCKGKRFNRE
                     TLEIKYKGKNIAEVLDLSVDEAYEMFESIPSIKNKLHVLRDVGLGYIKLGQSATTLSG
                     GEAQRVKLSSELSKRGTGRTLYILDEPTTGLHMADTHMLIEVLNRLVDKGNTVLIIEH
                     NLDVIKSADYIIDLGPDGGDAGGKLIAFGAPEEVAAIEGSYTGEYLKKVLPKTSIGKT
                     RRKEHTTPVQLPVRPFEEAKTQKKAGGRKLKIVDDDTSEEAIQTFISELEAQEEKHQE
                     "
BASE COUNT         1595 a         1277 c         1316 g         1741 t
ORIGIN      
        1 atcgtcaatc caccgaaaca agatatttct ggagtaaaaa tcatctctcc aataaatggt
       61 caaattataa atcagggtga tttttttgag gcaaaagcag aaataccgct aaaaggtcaa
      121 ggaacatacg agatttctgg taagtggatt gttgatggag tcagtcatcc tttcagccaa
      181 aaaatgcagg ttgcatggca ggcagttgca aaagtatctc aggtgcttcc aacgaatgtt
      241 tctggcgatc acaccgttga gctgatagtt gatttgccga cagcatacaa atcaggaatt
      301 attaaataca cagtttcttt aaaagcacca atagcagttg tacttgatcc tgtcattcag
      361 gatgcaactg tttcaatcga tggaactaca aaaatcaaaa ttaagataaa tgttacccag
      421 gcaggttctt tcaacttgct tggttacctt ctcctcgatg gtaaagtgta tgataaactt
      481 gaaaagattg ttattggtcc tggtgaattt atttatgaaa tgactatacc cgcccagtct
      541 gcgggtccgc atactgtcca gtttaaactc gtttctccga cagaactgct ttctaatgct
      601 gtaactttta gggttgttgg agaagcatgg gtatggcccg gcataatcta ccctggcgat
      661 gggacgactg ttacccagaa ctcgccgctt gttgcagaac ttgggatcaa ggttggtgga
      721 aaaggcacca tcaaagtggt tggcgtatgg atgatagatg accttccata tcgtgattat
      781 gagcagatgc tccaaataac atcggaaaca ataattctgg aaaagatgcc ccttccaact
      841 gatgctcctg gctggcacag actgaaattc aggatgacct ggccgtatta tgaggagaca
      901 aaagaaatct ggtacaggat atgggggaga gagcaaccac cgagtttcct gcaagtcatt
      961 acacatccgc agtcaccata ttcaaactac cagagttttc agctccaggt gagagctcag
     1021 gacgataggg gaatcaaaaa tctctgtgct tatgtcgatt ttgccaagca tgttgatgta
     1081 tcaatgggtg gaatgatggt tatcgactat ataacgccta ccattggccc gcttccggtt
     1141 ggcgagcaca catggtctgt tacactcaca gatcttgatg gactcgaatc agaatacata
     1201 gggaagtttg ttgtcacgaa atcttctggt gttctggagg gttttgtgct taacaagata
     1261 accaacgggc caattgtcga tgcagaagtg tcttgcgctg gacatctggt gaagacagat
     1321 gcttatggaa aatacaggat cgatgggctt ggtgaaggtg aacaggtggt catggccact
     1381 catcagtcat acaggagtgc tgaagcaagg gtcacaattt ttgctggtgt gaccacaaat
     1441 gcaccaacgc tttatcttga tgacaaaggc ccattaccct ttgtttatca gatagagcac
     1501 tggccgcagg ttccaaagac tggtgagagt tttgtcttga gtgtatatgt gaggaatgat
     1561 ggcgctgatg ctgatctttc ggaagtagca atatcctgcc cggaaggcgc agtaattgaa
     1621 attgatcctg atactggggc ccagtatcca tcgtttgcct atgtctatta tcctgggtct
     1681 ttcatagatc acagagatgg ccagcaaata agagcaatcc atcctgttgc cattgttagg
     1741 tggaattctt ggggcagtgg cgtgacaaga aaggtaatga tcaaggtgtc tccgcaggaa
     1801 cctaaaccat tcaatttctg ggtcagggct atgatgtcaa aaggtctgtc aaaagagatt
     1861 ttcccgagaa attctgaaat aatcgaccag cagggttatc caattaaaga atacagggtt
     1921 gaggtgcaac cgaaataatg aaaaaatcgc tccttctcct tatcgttttc tctgttcttt
     1981 ttgcctcccc ggcaatcgct gaaccaccta ctgatccggg taattcaggc agggtgaact
     2041 ttattgggcg tgagccaatg acgatgagat ggaagaagca tgtggattat gaaacagaag
     2101 agctaccatg cgcaactaat ggtaaacacg ttcttgttgt ttctggccaa tatagcatag
     2161 tttgtctgga tgccgataca ggaaaccaac tatggaaaag acaaacagtt gattatgtaa
     2221 aatacacccc ttgcgtaaca tcgaccatgg gccttgtnnn nnnnnnngtg accagcaagc
     2281 ttggttacat acaggccttt gatcttgaga ggggggaaca gaagtttctt atcaatcttg
     2341 gttttgacga gcttacaaca ccggttattg gtgaaaacta cgtttttctt aaaggtctca
     2401 atcacgttgc cagaacatcg tctgctatgg ccattgacct ttataaaggc agagttgttg
     2461 ggagctatgt tcaatgcgat tattccctgg agccgccagt tttgtcccaa aaagaagttt
     2521 ttgtagtgac accaaacagg aaaggtatcc aggcaatctc gcaatcaact ctccttacca
     2581 tatggacaaa gaatcttaat gatgaagtga cccatatttc aacaaggatg aaccatgttg
     2641 ttgctgtttg caggtctgga aacgtttaca caatggacaa tgctactggc aatatagttt
     2701 ggaagaccaa tgttacgggc ccggtaatct ggccagctac catttcaatt gatcatgtca
     2761 taatttgttc aggatcaaac gtcatttact cttttaggct ttcagatggc acccagcttt
     2821 gggaaaaacg ttggcctgtg actgttcaac ctcaatcatc aagagcatat gatatcttct
     2881 gttttggcaa aatgatgagt tttcatgatc caaaaagtgg cgaaaaactc tggcaacttg
     2941 aattgccatc tgaaacaata ggccaaccga tacctttcct ggaccaaata ttcgctgcca
     3001 caaaatcaaa caaaattgtt gccctcaaaa cagcaggttt tgaaatctcg ctttcctcaa
     3061 agaaacttga tttgggaagt gtttcttcaa gtcatcccta taccacgtcc caattaacag
     3121 tgaggaataa ttcaggtgag attcagcaga ttttcacatc atcgagtaac ccatggatat
     3181 cagtttcgca caatggtttt gagattatgc catttgatca gattgatata gccattgagc
     3241 ttgacatgca ttcgttaacg cttggacagt cgcagggtga aattgttgtt gcctggaaaa
     3301 atggctcgat aaaagttgac gtcatggcta aaaaaattgg cgataaggaa gctgtgcctc
     3361 caaaaccagg ttttctttcg cttggtacta ccgaggcaac ccttgagggt agattgaaca
     3421 tgggtgttcc acttgcttca ataacacttt tcaacaaggg agagcttccg gtctcgtata
     3481 ttgcaaaatc cgaagtccct tgggttgtta ttggcaggcc aaatggcagg atatatggca
     3541 atacaagcac atattttact gcaacaatta tcaatgatct tgcttcagtt ggcgagaaca
     3601 ctgcagaaat atatgcaact tgccctgaaa caggacaatg ctttactatt ccactaaaat
     3661 atactagaag ccttggcagg acgcatgtca tattccagat gcgcattggt tcttctgttg
     3721 gtcatcttgg acaatcgaaa ataagggtga ggccagaacc atttattgaa aacaactcca
     3781 tcatggttcc cctggaaatt ttcaagcttg cgctggacgc aacccttgat gtatacgaat
     3841 acacctatga accaacctat tatgtattgc aaaggggaga catcaagata acacatagac
     3901 ttggtgagtc aaaggtttat gtgcaggagg gcgctggtga gataaatgaa atactgctac
     3961 cgtgcccatc aagactcatc aatgagaggc cggttgtacc acttttgggg attgcaatgg
     4021 ccctcaaggg tgaaatgatt tactcacaag cagacggcaa agttactctt gatgtttttc
     4081 ttcctgagct tccaactcag aaataaatgt ctggattgct tcttcgctag tatcgtcatc
     4141 gactatcttt aattttctcc cgccagcctt tttttgcgtc tttgcttctt caaatggtct
     4201 gactggcagt tgaactggcg ttgtatgctc ttttcttctt gttttcccga tgcttgtttt
     4261 tggaagaact tttttcagat attcaccggt atatgaacct tcgattgcgg caacttcttc
     4321 aggtgcaccg aatgcaatga gctttccgcc agcatctcca ccatcagggc caagatcaat
     4381 gatgtaatca gcagatttaa taacatcaag attgtgctca attataagca ctgtgttgcc
     4441 cttgtcgacc aatctgttga ggacctctat gagcatgtga gtatccgcca tgtgaaggcc
     4501 ggtggttggt tcatcgagga tataaagtgt cctgcctgtc cccctcttgg acaattcgct
     4561 tgagagtttg accctctggg cttctcctcc ggagagtgtt gttgccgatt ggccaagttt
     4621 gatgtagcca agaccaacat ctctcagtac atggagcttg ttttttattg atggtatact
     4681 ttcaaacatc tcgtatgctt catcaaccga gaggtcgagt acttctgcga tgtttttgcc
     4741 cttatacttg atttccagtg tctccctgtt gaaccttttg cccttgcaaa cttcacatgg
     4801 cacataaacg tcaggaagaa attgcatctc tattttgatc attccgtcgc cttcacaggc
     4861 ctcgcacctt ccgcccttca cattgaacga gaaccttcct gcctcatacc ccctcgcttt
     4921 ggattcgggt aattttgcaa agagttctct gaggtaggtg aagagccctg tatatgtagc
     4981 tgggtttgat cttggcgttc tacctatcgg tgactggtca acaatgatga ccttatcgag
     5041 attgcggatt ccttctattt tttcgcactt gcctggcatt tcccttgaag catagaattc
     5101 ttttgcgaga ctggcataca ggatgtcatt tacaagtgta cttttccctg atccggaaac
     5161 gcctgttaca acggtcaggg tcccaagtgg tattgttatg tcgttccctt taaggttgtg
     5221 ttctgtgggg ttgacgatct ttatggatct tccgtcacca gaccttcttt catctggaat
     5281 ttccaccttt tttttgccgg acagatacat accagtttct gatatgggaa ctttgcagat
     5341 ttctgatacg cttccagttc caacaacgtg accgccgttt actccggcgc caggaccaat
     5401 atcaacgata aagtcggctt cccttattgt ttcctcgtca tgctctacaa caattaatgt
     5461 attaccaaga tctctcaatc ttttgagggc agaaagcagt ttgctattgt cttttgaatg
     5521 gagtccaatt gacggttcgt caagaacata aagaacacca ctaagactgg aacctatttg
     5581 tgttgccagc ctcaaccttt gggcctcgcc tccagagagt gttgccgacc ccctgtcgag
     5641 actcaaataa gccaacccga catcaagaag aaagttgagc ctggatttta tttctttgac
     5701 tacctgtttg gaaatggcca tgtttttttc tgaaagtttc agcatttcaa aaaaattgaa
     5761 tgctttgtct attggccatg aggcaatttc tgcaatattc ttcccgctga cctttactga
     5821 taatgcttct ggtttgagtc tttttcctcc acataccggg caggggacct caatcataag
     5881 ctcttcatag gcctggcgca tgccatcact ttgtgtctgt ttgtgtctgc gctgtattg
//
LOCUS       DAOSRI010000086         5890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12381_length_5890_cov_4.295116, whole genome shotgun sequence.
ACCESSION   DAOSRI010000086 DAOSRI010000000
VERSION     DAOSRI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_5890_cov_4.295116"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..807)
                     /locus_tag="PK717_03585"
     CDS             complement(97..807)
                     /locus_tag="PK717_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46777.1"
                     /translation="MKKISLALAVLFIVQIGFGSVFAFETKTTAKGKVNESNPGAIVV
                     DVTPIIGEKAPFIQIYSRENQKKIRNIADRALKEIAKGAPAGFNFDPLVLYKHNGKIM
                     TSPLPQNTYRNDNPILPTQKIAPSGIIDWRFTGSCQSGATPPDSYWNCATEMPNIQTF
                     VEGGPGLPIGMKNCIQDVFGAPMFSIEVYFRRDDNLPTGTFAQYTQSGVGTIGYIDMP
                     TDMARPPAYFVPAWNMMA"
     gene            1469..1651
                     /locus_tag="PK717_03590"
     CDS             1469..1651
                     /locus_tag="PK717_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOR46778.1"
                     /translation="MKPTVDKETCIGCGVCASVCPDVFEIGSDGLAGVISGSDCGGCG
                     CCEEAAENCPVAAIKL"
     gene            complement(1714..2424)
                     /locus_tag="PK717_03595"
     CDS             complement(1714..2424)
                     /locus_tag="PK717_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46779.1"
                     /translation="MNPTNERELKLDINADASPKISTLISELIEKYKWPVRARIDMIN
                     YDRYFDQLQPSGLFLLHSQDAYIRVRKSVWPQKSREAEFIAYRKLNEASYGKPAEIYD
                     NEISVLKTNEIIAELLRDHPEKLCPPSPEAIGVVEHLESMGLSEIIRLKCERCIYPIS
                     YSDQPNDQVKIKIDRVTYKARPNEVFTQVEIDYYSNELFDMATKIMEVINVGVRDITG
                     IDSRFSSVSKMSRGIELL"
     gene            complement(2421..3071)
                     /locus_tag="PK717_03600"
     CDS             complement(2421..3071)
                     /locus_tag="PK717_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOR46780.1"
                     /translation="MVQFENESKYMVLKNKKAVEEAIEKISVELGFSLGDIRFVDQLD
                     VYLDCDFVLKNSGYSLRNRYEGGILKKITLKTLETFDEKNHIRIEEESNTYKDLMGKV
                     TKKLKSIFQDPADETTLKLLSEPEKLLPVVVLTKGRKTREICCSKGCGQIAFDTYTYV
                     FPKTSNVFSEIEVEGPAFLDTLRQAIEKKFVKNKNPTLGPSQKSKFLLGLNNGGEQ"
     gene            3157..4173
                     /locus_tag="PK717_03605"
     CDS             3157..4173
                     /locus_tag="PK717_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR46781.1"
                     /translation="MNPDFDYISKGLAGVEENLSKAALYPIGQALELAISLIPLGLPS
                     DSRFWLEKLAQMSRNSQSSGKRFRAKLALASSQAIGLPVDMAINLGAFVEMIQSASLI
                     HDDVVDEASLRRNEPTVNSLMGNRFAVLTGDYVLSLALTKLGELKNHNLTMRFSEVVS
                     QMTFGEAMEIEITFDHTRTIAHYLTTISLKTASLLSFCCQSACLIANVDEKTINSLTE
                     FGANLGMAFQLIDDTLDFVGPDGKEKCQDFKEGLLTLPILLLDIQGNPFDKSIDEIQV
                     LIAEKNTLTQTIDKAREYSVKAIDCLDGIRSLIPEHCLQTFTNINTSIYERLPNNLNP
                     FPRR"
     gene            4176..4784
                     /locus_tag="PK717_03610"
     CDS             4176..4784
                     /locus_tag="PK717_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008798468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOR46782.1"
                     /translation="MSVDEMIQKTWNNSNGLDINVGVSSRHIHLHEDHVKILFGHDLT
                     CYKDLTQPGQYACNEKVDLVGPKGTIKGVRVLGPTRRATQVEVSKTDSIILGLDVPLR
                     MSDDIEGTPGIKLVGEKGEIVLDKGVIVAKRHIHMSPEDAEKAGVTNGEMVWVSANTP
                     RKLVMGDIVIRVSKTFALDFHIDTDEANAAWLKSGDKIRIVR"
     gene            4788..>5890
                     /locus_tag="PK717_03615"
     CDS             4788..>5890
                     /locus_tag="PK717_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46783.1"
                     /translation="MTKEKIENIQQSTDTFSIQYEKNQIDNKGVRFSTMLLAAKYAAM
                     GLLILLTYITGFARYIGDWAIALGGSADNFVIWFGFGFAYSICLSVLVFPIDWYREAV
                     VQVKSGVHGMPWVNTLGRILRRDFFDALLWGGLNYYFLVMYFSLSTTGRQGPMGGNTP
                     QWWIISALWGMLFVPALFIIINLLKLITDNSVKQVPTGPDRRIIKELGRKFRVTVLDV
                     FYYNLGGDFNAETKLVGLFGVYFVFAPAKLKGKDVIIASVAKAMARADVLKGTVINIV
                     RWVFFVAGSYLIYLAFNWVYVNNVFSWFSEQAGTGDPAVGIIVMFIIGALYLIATPLI
                     NLISKRMDIGADKLASRAIGRPEVYSNYLKMVDT"
BASE COUNT         1740 a         1237 c         1217 g         1696 t
ORIGIN      
        1 ctctgtcatt ccttcaaccc atgcgtggaa gtaaaggttg agattgtcat agtaggcatg
       61 gaccatttca tgtgtcagtg catcaagcct tgcttcttac gccatcatgt tccaggctgg
      121 tacaaaataa gccggggggc gtgccatgtc cgttggcata tcaatgtaac ctattgtgcc
      181 gacaccagat tgtgtgtact gggcaaaagt gccagttgga aggttatcgt ctcttctgaa
      241 atagacctca atggaaaaca tcggggcgcc aaaaacatcc tggatacaat ttttcattcc
      301 aattgggagg cctgggccac cttctacaaa ggtctggata tttggcattt cagtcgcaca
      361 gttccagtat gaatcaggag gtgtagcacc agactggcat gaacctgtaa atcgccaatc
      421 tataatgcca gaaggtgcta ttttttgggt tggcagtatt ggattatcat tgcggtatgt
      481 gttctgcggg agagggctag tcattatttt cccgttgtgc ttgtagagta ccaatggatc
      541 aaaattgaat ccggcaggag ctccctttgc aatctcttta agggccctgt cagcaatatt
      601 cctaattttt ttctggtttt ctcttgaata gatctggata aacggagctt tttctccaat
      661 gatcggggtt acatcaacca cgatcgctcc agggttcgat tcgtttacct tgcccttagc
      721 agtagttttt gtttcgaagg caaacacaga accgaaacca atttgcacaa tgaacaacac
      781 agctagcgcc aatgaaattt ttttcatgct aactcccctt tttttacgaa aaacaaccga
      841 atggtctaga atagaaacag cttgaaaacg acaaatacga gcagtaccaa tacgatacaa
      901 attaagatta ttaacgaaca attacaattt attgatattg gaatgaattg tcaagtggcc
      961 actcttggga taatttccaa tgcaaatctt tctttggcca aaatttccaa acaaatataa
     1021 aaaaccacca caaaatggtc tatttgagat ataatagcac atttttgtat ctcaatgtta
     1081 catatgtgca tatcaaattt attaagctat tcatatacat aaaaattggg caagaatcat
     1141 ttataaaaac atggaggttc tcatttattt aggtttggat ttataaacaa tatttaaatt
     1201 tatgactaaa attagtaatt tatatatcaa aaattaattt aatgctaaat cggtaaacag
     1261 tactcatatt tgatattaat aatttttact ggattggaaa taaatcattc aaacagtttg
     1321 gtataaacct atggaaaaga aaaaatgcca taggtttgcc aaaagcacgt ttcttttaat
     1381 tttaaaagcg aacaaaaact tcaaagataa cttgacacac aaatccccaa aaatatacta
     1441 cgcatgcatt acaaattaag gaggaaccat gaaaccaaca gtcgacaaag agacttgcat
     1501 cggttgcgga gtttgcgcct cagtttgccc ggacgttttt gaaattggaa gtgatggcct
     1561 tgcaggcgtt atcagtggct cggattgcgg tggttgcggt tgctgcgaag aagctgccga
     1621 gaactgccca gttgctgcta ttaaactctg agaaaacaga aacaaaaagc ggccctctaa
     1681 ggggccgctt ttttattgaa tgaaaaagtt tttctaaaga agctctattc ccctgctcat
     1741 ttttgaaacc gacgagaaac gcgaatctat acccgtgatg tctcgcaccc caacatttat
     1801 cacttccatt atttttgtag ccatgtcaaa aagttcgttc gagtaataat cgatttcaac
     1861 ttgtgtaaac acttcgtttg gtcttgcttt gtatgtcacc ctatcaattt ttattttgac
     1921 ctggtcattg ggctggtcgc tgtaagaaat aggatatata cacctttcac acttcaacct
     1981 gataatctct gaaagcccca ttgattcaag gtgttccacc acgcctatgg cctccggact
     2041 cggtgggcaa agcttttcag gatgatccct gagaagttcg gcaattattt catttgtttt
     2101 caagactgat atttcattat catatatctc ggctggtttc ccatagcttg cctcgttgag
     2161 tttcctgtag gctatgaatt ccgcttccct tgatttttgt ggccacactg attttctgac
     2221 tcttatgtag gcatcctggc tgtgcaagag aaacaatccg gagggctgaa gctggtcaaa
     2281 atacctgtca taattgatca tgtctattct tgccctgacc ggccatttat atttttcgat
     2341 aagctcagag ataagagtgg aaatcttcgg cgaggcatcg gcattaatat caagcttaag
     2401 ctctctttca ttggttggat tcattgttct cctccgttat taaggcccaa aaggaatttg
     2461 cttttttgcg atggccccag ggttggattt ttgtttttga caaacttttt ctcgatggct
     2521 tgtctaagag tatcaagaaa agccgggcct tccacttcta tttcagaaaa tacgttgctg
     2581 gtttttggaa aaacataagt atatgtatca aaagctattt ggccacaacc tttactgcag
     2641 catatttctc tggtttttct ccccttggtc aaaacaacaa cgggcaaaag cttctccggc
     2701 tcagagagca attttagcgt agtctcgtct gctgggtcct ggaagattga tttcaatttc
     2761 ttggtcactt tgcccataag gtctttgtag gtgttggact cctcctcaat ccttatatgg
     2821 tttttttcat caaaagtctc aagggttttt agggttattt ttttcaaaat gccaccctca
     2881 taacggtttc tcaatgaata tccagagttt ttcaaaacaa aatcgcagtc aaggtagacg
     2941 tcaagctgat ctacaaacct gatgtcaccc aggctaaatc caagttcaac ggaaatcttt
     3001 tcaatggcct cttcgacggc ttttttgttc tttaaaacca tatactttga ttcgttttcg
     3061 aattggacca tttttaatgg taatataatg ccgctttcag ccaagtcaac gactgatgcc
     3121 ttgatttgac agtgatcaca actaaaatga ccgtttgtga atccagattt tgattacata
     3181 tccaaggggc tagcgggcgt agaagaaaac ctgtcaaaag ccgctttata tccaataggg
     3241 caagcattag agcttgctat ttctcttatt ccactgggac tgccatcgga ttcacgcttc
     3301 tggcttgaaa agctcgccca gatgtcaaga aatagccaaa gttccggcaa aagattcagg
     3361 gcaaaactgg ctcttgcatc ttcccaggcc ataggtcttc ccgtagacat ggcaatcaac
     3421 cttggcgctt tcgtagaaat gatacagtcc gcaagcctca tacacgacga cgttgtggac
     3481 gaggcatcat taagaagaaa tgaaccaacc gtaaacagcc tcatgggcaa ccgttttgcg
     3541 gtgcttaccg gcgattatgt tctttcttta gctttgacaa aattgggtga actaaaaaac
     3601 cacaatctga caatgcgctt ttccgaggtc gtatcacaaa tgacgttcgg tgaggcaatg
     3661 gaaatagaaa taacgtttga ccatacaaga acaatcgcac attatctcac cacaatatcg
     3721 ctcaaaactg cttcactcct gtcattctgc tgtcaaagcg cctgcctgat cgcaaacgtt
     3781 gatgagaaaa caataaacag ccttacagaa tttggtgcaa atctcggaat ggcctttcag
     3841 ctaatagacg acacgcttga ttttgtgggc cctgacggga aagaaaaatg ccaggatttt
     3901 aaggaaggcc ttttaaccct acccatttta ttgttggaca tacagggaaa cccgtttgat
     3961 aaaagcattg acgaaataca ggttctgata gcagagaaaa acactcttac acaaacaatt
     4021 gacaaggcca gggaatattc cgtaaaagca atagactgtc tggatggaat aaggagcctg
     4081 atcccagaac actgcttgca aacatttacc aacataaata caagtattta tgaaagactt
     4141 cccaataatc tcaacccatt tccaaggaga taaatatgag cgtagacgaa atgatccaga
     4201 aaacatggaa caatagcaac gggctcgaca tcaatgtcgg tgtaagctca agacatattc
     4261 atctgcatga ggaccatgtc aaaatactct ttggtcatga tctgacatgc tacaaggacc
     4321 tcactcagcc agggcaatac gcatgcaacg aaaaagttga cctggtcgga cctaagggaa
     4381 ccatcaaggg agtcagggtg ttaggcccaa ctagaagggc aacacaggtt gaggtttcaa
     4441 aaaccgactc tataatactt ggtctggatg taccactcag aatgtcagat gacatagaag
     4501 ggactcccgg aataaagctg gttggagaaa aaggtgaaat tgtcctggac aaaggagtca
     4561 tcgttgcaaa aagacatatc cacatgtcac ccgaggacgc tgaaaaagct ggcgtcacca
     4621 atggtgaaat ggtctgggtt tctgccaata caccaagaaa attggtaatg ggtgacatcg
     4681 tgataagggt ttcaaaaacc tttgcactcg attttcatat tgacacagat gaggcaaatg
     4741 cagcctggct caaaagtgga gacaaaatca ggatagtgag gtaactcatg acaaaagaaa
     4801 aaatagaaaa catacaacaa agcaccgata ctttttccat acaatacgaa aaaaaccaga
     4861 ttgacaacaa gggtgtacgc ttttcaacaa tgttgcttgc ggcaaaatat gctgctatgg
     4921 gactccttat acttctgacc tacatcaccg gtttcgcaag atacataggc gactgggcga
     4981 ttgcacttgg cggaagtgcc gacaattttg taatatggtt tggtttcggt ttcgcttact
     5041 ccatttgcct ttcggtactc gttttcccaa ttgactggta cagggaagca gtcgtgcaag
     5101 taaaatccgg tgtccatgga atgccatggg taaatacgct gggaagaatc cttcgcagag
     5161 atttcttcga tgccctgctt tggggtggat tgaattatta cttcctcgtt atgtatttct
     5221 cactctccac aactggaaga cagggtccca tgggtggcaa caccccacaa tggtggataa
     5281 tttcagctct ctggggtatg ctttttgtcc cggcattgtt cataataatc aatcttctca
     5341 aattgattac tgacaactca gtaaaacagg ttccaacagg tcctgacaga aggataataa
     5401 aagagcttgg aaggaaattt agggtaaccg tcctcgatgt tttctactac aaccttggcg
     5461 gcgattttaa tgctgaaacc aagcttgttg gtctctttgg cgtatacttc gttttcgctc
     5521 cagcaaagct caaaggcaaa gatgtcataa ttgcgtccgt tgcaaaagca atggcaaggg
     5581 cagacgtatt gaaaggcaca gtcattaaca ttgtaagatg ggtcttcttc gtagctggtt
     5641 cttacttgat atatctcgct ttcaactggg tctatgtaaa taatgtcttc tcctggttct
     5701 ctgaacaggc aggaaccggt gatccagcag ttggaatcat agtaatgttt attattggcg
     5761 ccctctatct cattgccact ccgctcataa atctcatatc aaaacgtatg gacatcgggg
     5821 ccgacaagct cgcgtcaagg gcaattggca gaccagaagt ttattcgaac tatctcaaga
     5881 tggtggacac
//
LOCUS       DAOSRI010000087         5889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12387_length_5889_cov_3.471203, whole genome shotgun sequence.
ACCESSION   DAOSRI010000087 DAOSRI010000000
VERSION     DAOSRI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12387_length_5889_cov_3.471203"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PK717_03620"
     CDS             <1..1172
                     /locus_tag="PK717_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR46784.1"
                     /translation="GGDLFKARFETDFYTDPNNFNVRQDFIDWTVGPYLMKQWEIGDH
                     IEYKPNPYAAFAQPLIEKIIVKFMPDPNTIRLNLQADNIDIVTSTFSPLDAEELMKKV
                     PKSRFYFTETTSWDHIQLNQFKDEKGKYDIFGDKRVRQALLYGLDRPQISKAAAAGIY
                     QPSHSWITKKSPYYDESSVKKYEYNPAKAEQLLEEAGWKLANVDGKNIRCKNGDPSKP
                     LKFSLSFAQESDYRLRVAEQILRMWERIGVWVVPQARPAKELLGGDVLSGHLFESMML
                     GLVSNPIRPNANLWRSDQIPTKQNNGAGQNYGGWVGSKEHDELCYEIEKIQPEAKLRG
                     LFNKEVAIWCDELPALPLFNRYDVDVATRDIQNIKPTGANRTINWNCEFWYREVK"
     gene            1502..2380
                     /locus_tag="PK717_03625"
     CDS             1502..2380
                     /locus_tag="PK717_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46785.1"
                     /translation="MRRFLGVFVCFMLAVGLFAYAVPAKAATQEHWATKYVSEMTSDG
                     VKALAPREDWDSEITAADFVKGLSVILGKDIPAPEGTFNRAIMVKLAVDNHTYAEQLK
                     DFDFPAWCLSNDEETIPKDFVPYFNMAYRPIHQLLTYRKNRVTAWDKTPLFSEAAYLL
                     YMLKYPPNTDPGQQIVVVTAQEPDTLNPFTTSALSATLLGTFYGAGDITYDSDATKYP
                     NAALRVPTLDNGDVKLFKDPITGKDKMTVTYRFRPGMYFPPLPGEEEGSNSTKLPLMT
                     PFLVLGSQFLLSFSQL"
     gene            2365..3645
                     /locus_tag="PK717_03630"
     CDS             2365..3645
                     /locus_tag="PK717_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR46786.1"
                     /translation="MQSVVRTGILKYDYVKKVDKYTIEVGYNEVYVFGTWGIGWTFKA
                     KFEPDFYTDPGNFNVRDDFRDYEVGPYRIKTWERGDHIEFEPNPYAIFAQPIVKKIIV
                     KFMPDANTIRLNLQGGNVDITTNAFDPTDASELEKKLPRVKFYYTPGTSFEHVTPNLF
                     EDETGKAFYFGDVRVRRAMLYALDREQLTKITSGGIFSVAHSWLTPKSKYYDDKALVK
                     YEYNPAKAEQLFEEAGWKLANVDGENIRCWQGDPTKPFKVTLATTSENPFRQKNVEEM
                     IKMWARVGLKIIPQLRPSKELFGGKVLSQHQFELVEFAWVSNPVRPNALMWRSDQIPT
                     QANNWSGQNISGWKGNKENDDIVSELEKELPDAKLQELLNKQIRIWGENLPILPLFNR
                     YDVDTATRDIQNIKPTGSQRTINWNCAFWYREKK"
     gene            3735..4703
                     /locus_tag="PK717_03635"
     CDS             3735..4703
                     /locus_tag="PK717_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46787.1"
                     /translation="MGNYLFRRFVIFIFLFILVTVLCYFLLRLPGDPYKELELQQPNM
                     KDEIARMREWSGFNDNWFVGYTKWAKKIIFDGDWGKSLYDKQPVWGKIITKIPVTLKI
                     QVSVLILGLLLGIPLGIFSARRHYSVADYAITVFSYIGISVPNFWFGLLLMLLFSVNL
                     GWLPAGWSLRPDYDSLSWIMKILDQARNLVLPVFMGLLGTIASLSRYMRSSMLEVIRQ
                     DYVRTARSKGLSETKVIYKHALRNGLIPIVTVMTLVIPSLISGSVVVERIFNINGMGQ
                     LLFNSVQSSDIPLAMGTLVMFAFLTLISLILGDVVYALVDPRITYS"
     gene            4712..>5889
                     /locus_tag="PK717_03640"
     CDS             4712..>5889
                     /locus_tag="PK717_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR46788.1"
                     /translation="MATKNKKKKVTVIDNPNEQEVLSSSEYSYIGMVWSRFKRHKLAT
                     IGLVLLVFMALFAILAPVFELITGYPVGHSSLAVKMSPFSTPGVVAKITHPDFGNPEK
                     PMGKVDFWFEITPDNNPEKTGFASVKESTFKNPDYRGFYDFNMKAYGNTNVLLTNDQV
                     VEKPDGSAEIVKGGYSEFFIERARNKRIVVYNQVVTYSPTFESVDSRTIEVQNGYKGP
                     REVDIGEAPMNVFGTDGLGHDVLVRLSYGARMTLMIAFVVVILETLMGIFLGALAGFY
                     GGFIDALIMRILELMMTVPTLPIYMVLSRFMGGGSAIGIIIILAAFGWTGVARMTRGM
                     FLSLRNQEFTEAARAIGASPMRIMFKHLLPNSLAPIIVGITFSIGGVILTESTLSFLG
                     L"
BASE COUNT         1731 a         1209 c         1311 g         1638 t
ORIGIN      
        1 cgggtggtga tcttttcaaa gcaaggtttg aaacagactt ttatactgac ccgaacaatt
       61 ttaatgtgag gcaagatttc atagactgga ctgtcggacc atacctcatg aagcagtggg
      121 agattggtga ccacatcgaa tacaaaccaa atccttatgc tgcgtttgca caacccctta
      181 tagaaaaaat aattgtaaaa tttatgcctg atccaaatac tatcaggttg aatctccaag
      241 ctgacaacat agatatcgtg acttcaacgt tcagcccatt ggatgctgaa gaattgatga
      301 aaaaggttcc taaatctaga ttctatttca ctgaaaccac ttcttgggac cacatacagt
      361 tgaaccagtt taaagatgaa aaaggtaaat atgatatctt tggtgacaaa agagtaagac
      421 aggccctact ttatggtctt gacagaccgc aaatttcaaa ggcagctgct gcaggtattt
      481 accagccttc acattcctgg ataaccaaga aatccccata ctatgatgag tcatctgtta
      541 agaagtatga atataatcct gccaaggcag aacagttact tgaagaagca ggatggaagc
      601 ttgccaatgt tgatggcaag aatatcagat gcaaaaacgg agatccaagc aaaccgctca
      661 aattttcact ttcttttgcg caagagagtg attataggct cagagttgca gagcagattc
      721 tcaggatgtg ggaaagaata ggtgtgtggg ttgtcccaca agcaagacct gccaaagaac
      781 ttcttggtgg cgatgtactc agcggccatc tttttgaatc aatgatgctg ggccttgttt
      841 caaacccaat cagaccaaat gccaatctct ggagaagcga ccagatacct acaaaacaaa
      901 acaatggggc tgggcaaaat tacggcggtt gggttggctc aaaagaacat gatgaactgt
      961 gctatgagat tgaaaaaatt cagccagaag caaaacttag aggactattt aacaaagaag
     1021 tggcaatttg gtgtgatgag cttcctgccc tcccactttt caatagatat gatgttgatg
     1081 ttgcaactcg tgatatccag aacatcaaac caactggcgc aaacagaaca ataaactgga
     1141 attgtgaatt ttggtacaga gaagtcaaat aaaatactgt gattgattcg gtaaatttac
     1201 ggtgaatcat gggaacattt gacaaatact tcccattgtt tgatgggagt agatttttgg
     1261 tcaccaagag ggtgtgctgg ttttgcacag aaatatcaat tttctcaaaa ctacacaccc
     1321 atttatctct acgcgtattg atatccttac aggcaagttt taaattggtt ccaaaagggc
     1381 ttgcagtttg ccacaaatca aatagaataa cctcgcatct tttgggcatg cagccacacc
     1441 acaacgatgg tggtgagtgg aacgtgctaa acattaaagc tttgccaatg aaaggagtgc
     1501 aatgaggaga ttccttggtg tgttcgtttg ttttatgctc gcagttggcc ttttcgctta
     1561 tgctgttcca gcgaaagcag ccacacagga gcattgggct acaaaatacg tgtcagaaat
     1621 gacaagtgat ggtgttaagg cccttgcccc gagagaagac tgggactctg agatcacagc
     1681 agctgatttt gtaaaaggac tttcggtgat tttggggaaa gatataccag ccccagaagg
     1741 cacttttaac agagccatca tggttaagct ggcagtggac aatcacacat acgctgaaca
     1801 gctaaaagat tttgatttcc cggcatggtg tctttccaat gatgaagaga ctattccaaa
     1861 agattttgta ccatatttta acatggcata caggccaatc catcagctcc ttacctacag
     1921 gaaaaacagg gttactgcat gggacaaaac cccacttttc tctgaggccg catatcttct
     1981 ttatatgctc aagtatccac caaatactga tccaggacag cagattgtcg tagtcacggc
     2041 acaggagccg gacacactta atccattcac cacctccgcc ctttccgcaa cgcttcttgg
     2101 aacgttctat ggcgctggcg atatcactta tgatagtgat gcgaccaagt atccaaatgc
     2161 agcacttagg gttccaacac tcgacaatgg tgacgtaaag cttttcaagg acccaatcac
     2221 aggcaaagac aaaatgacgg ttacatacag gttccgccct ggcatgtatt tccccccact
     2281 tccaggtgaa gaagaaggtt ctaactccac gaaattaccg ctgatgacgc cctttttggt
     2341 tttagggtcg cagtttctcc tgtcattcag tcagttgtaa gaacaggaat cctgaagtat
     2401 gactatgtga aaaaagttga caaatacaca attgaagttg gttataacga agtttacgtt
     2461 tttggaactt ggggcattgg ctggacattc aaggcaaaat ttgaaccaga cttctataca
     2521 gatccaggca attttaatgt cagggacgac ttcagggatt atgaggttgg accatatcgt
     2581 atcaaaacat gggagagggg cgaccatata gagtttgaac caaacccata cgccattttt
     2641 gcacagccaa tagtcaaaaa aatcatcgtg aagtttatgc cagatgcaaa caccatcaga
     2701 cttaacctcc agggtggtaa tgttgacatc acaacaaatg cttttgaccc aactgatgct
     2761 tctgaacttg agaagaagct tccaagagta aaattctact atacaccggg tacttctttt
     2821 gagcacgtca caccaaacct ctttgaggat gaaactggaa aagctttcta ttttggtgat
     2881 gtcagggtca gaagagccat gctttatgca ctggacagag aacagctcac aaagataaca
     2941 agcggtggaa tattctcggt tgcacattca tggctgacac caaagtcaaa atattatgac
     3001 gacaaagcat tagtgaagta tgaatacaac cctgcaaaag cagagcaact ctttgaagaa
     3061 gcaggctgga agcttgccaa cgttgatggt gagaatatca gatgctggca gggcgaccca
     3121 accaaaccat tcaaggttac acttgctaca acttctgaaa acccattcag acaaaaaaat
     3181 gttgaagaga tgataaagat gtgggccaga gttggcctta agatcattcc acagcttcgc
     3241 ccatcaaaag agctctttgg tggtaaggtt ctctcccagc accagttcga acttgtagag
     3301 tttgcatggg tctccaaccc agttcgtcca aatgctctaa tgtggagaag tgaccaaatc
     3361 ccaacccagg ccaacaattg gtcaggtcag aatatttctg gttggaaggg caataaggag
     3421 aatgatgata ttgtatccga gcttgaaaaa gagcttccgg atgccaagct ccaggaactc
     3481 ctcaataaac agattagaat ctggggagaa aaccttccga tactgcctct gttcaacaga
     3541 tatgatgttg ataccgcaac tcgcgatatt cagaacatta aaccaactgg ttcccagaga
     3601 acgattaact ggaactgtgc tttctggtac agagaaaaga agtgatgtga attctgcgcc
     3661 ctgggttttt tcgcccccca gggcgctctt ttttagttta agcggcgggc tttggcccag
     3721 aaaggtgaag acagatgggt aattatctgt tcagaagatt cgtaatcttc atttttttgt
     3781 ttattcttgt taccgttctg tgttatttcc ttcttagatt acctggagac ccatacaaag
     3841 aacttgagct tcaacagccc aatatgaagg atgagattgc caggatgagg gaatggtcgg
     3901 gatttaatga caattggttt gttggttaca ccaaatgggc caaaaaaata atttttgatg
     3961 gtgactgggg aaagtcgctt tatgataaac aaccagtttg gggaaaaatc ataaccaaga
     4021 ttcctgtcac gctcaaaatc caggtttcag ttctgatttt gggcctgctg ctgggtatcc
     4081 ccttgggaat tttctctgca aggcggcatt actcagtggc tgactatgcg attactgtgt
     4141 tttcgtacat aggaatatca gtgcctaatt tctggttcgg tttgcttttg atgcttctct
     4201 tttcggtgaa tttggggtgg ctccctgccg gatggtcttt gcggcctgat tatgattctt
     4261 tatcatggat catgaagata ctagatcagg ccagaaacct tgttcttcct gtgttcatgg
     4321 gccttcttgg aacgatagcc tctctgtctc gctatatgag atcgtctatg cttgaggtta
     4381 tccgccagga ttatgtcagg actgcaagat cgaaaggcct ctctgaaaca aaagtcattt
     4441 acaaacatgc ccttagaaac gggcttattc cgatagtaac tgtaatgacg ctggtcatcc
     4501 cttcccttat ttctggttct gtcgttgtgg agaggatatt caatatcaat ggaatgggcc
     4561 aattgttatt caattctgtg cagtcatcag atataccact agcaatggga acacttgtaa
     4621 tgttcgcatt tttgacctta atttcgttga tacttggaga cgtagtgtat gcgcttgttg
     4681 atccgcgcat cacttactcg tgaggtgaga tatggcgaca aaaaataaaa agaaaaaagt
     4741 taccgtcata gataatccca acgagcaaga ggtgctctca tcttctgaat attcctatat
     4801 tggaatggtt tggagcaggt tcaagagaca caaacttgcc actattggcc ttgtattgtt
     4861 agtgtttatg gccctttttg cgattcttgc gcctgtattt gagttgataa ctggttatcc
     4921 agttggacat tccagcctcg ctgtaaagat gtctccattt tcaacccccg gtgttgtagc
     4981 aaagattact cacccagatt ttggcaatcc ggaaaagcca atgggcaagg ttgatttctg
     5041 gtttgaaatt acacctgata ataatcctga aaaaactggt ttcgcatcag ttaaagaatc
     5101 aacctttaaa aatcctgatt acaggggttt ttacgatttc aacatgaagg cctatggcaa
     5161 cacaaacgtt ctacttacaa atgaccaggt tgtcgaaaag ccggatgggt cagctgagat
     5221 agtaaaaggt ggctatagtg agttttttat agagagagcc agaaacaaaa gaatcgtagt
     5281 atacaatcag gtagtcacat attcgccaac gtttgaatct gtagattcaa gaacaatcga
     5341 agtccaaaac ggatacaagg gcccgcgcga agtggacatt ggtgaagcac caatgaatgt
     5401 ctttggaaca gatgggcttg ggcatgatgt cctcgtaagg ctttcttatg gcgccaggat
     5461 gacactcatg atagcttttg ttgttgtcat tcttgaaact ctaatgggta tattccttgg
     5521 tgctcttgcc ggtttctatg gtggttttat agacgccctc attatgagaa tccttgagct
     5581 tatgatgaca gttccaacac ttccgatcta catggttctc tcaagattta tgggtggtgg
     5641 aagtgctatt ggaattataa tcattcttgc agctttcggt tggactggag ttgccagaat
     5701 gaccaggggg atgttcctct cacttcgcaa tcaggagttt actgaagctg caagagctat
     5761 tggtgccagc ccaatgagga taatgttcaa acatttactc cctaactctc ttgcaccaat
     5821 aattgttggt ataacattta gcatcggagg ggtcattctt actgagtcaa cgctttcctt
     5881 cctcggact
//
LOCUS       DAOSRI010000088         5778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12664_length_5778_cov_4.346322, whole genome shotgun sequence.
ACCESSION   DAOSRI010000088 DAOSRI010000000
VERSION     DAOSRI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12664_length_5778_cov_4.346322"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK717_03645"
     CDS             <1..231
                     /locus_tag="PK717_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOR46789.1"
                     /translation="RMGRSMMKKLMKKTNVASLSEMLAMARDLDVKMWACKMSMDVMG
                     VAKEDLIPEVDKIVGVAAYAAEAQQSKINLFI"
     gene            246..479
                     /locus_tag="PK717_03650"
     CDS             246..479
                     /locus_tag="PK717_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOR46790.1"
                     /translation="MNFDVELNTLGMQCPVPIVKTAKKMKELQIGQILKVISDDEGIK
                     EDMPAWCKTTGNEFLGLEESGGQYTVYVKKVVG"
     gene            476..826
                     /locus_tag="PK717_03655"
     CDS             476..826
                     /locus_tag="PK717_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOR46791.1"
                     /translation="MKLGIVVLTTPYTFQNMDTAISLAGAALEKGHQAMIFLYMDGVI
                     AASNLIKPGNDRSIPIKMQSLIEKGVKITPCGVCCNYRGVRQADSQPGIKHAGIAALG
                     QLVSECDRVLTLGF"
     gene            830..1186
                     /locus_tag="PK717_03660"
     CDS             830..1186
                     /locus_tag="PK717_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOR46792.1"
                     /translation="MAKKVAIVVSKAPYGSAIMGEAFRAAMGLPSVGLETNVVLEGDA
                     VFALLKDQKPKEALDFWSLHDAWLQFEDYGFSLFVHKPSVEARGLTCEQLSHCQTLDD
                     SEFKKMLRDMDAILRF"
     gene            1188..1490
                     /gene="tusB"
                     /locus_tag="PK717_03665"
     CDS             1188..1490
                     /gene="tusB"
                     /locus_tag="PK717_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HOR46793.1"
                     /translation="MEKKMFIMLTKSPYGCEEGKIRISNALAGDVVVFAQDSVYAATN
                     PPGELAELVKSKMSEGIKFYASGPDCKARGVEPKFGIKQIGYPEMIDLIMERQLSY"
     gene            1772..2626
                     /gene="rpsB"
                     /locus_tag="PK717_03670"
     CDS             1772..2626
                     /gene="rpsB"
                     /locus_tag="PK717_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOR46794.1"
                     /translation="MSVVNMKHLLEAGVHFGHQTKRWNPKMRPFIFTARNGIHIIDLR
                     ITAEKCEQAYATVRQIAQSGGNIVFVGTKKQAQDIVKDEAKRCGAHYVNQRWFGGTLT
                     NFITIHTRIEKLRELEEKEVTGELKRLPIKEYMGYKKEREKLQKFLDGLRNMDTLPQA
                     IFVTDTHKDRAAIIEARKQNITVIAIVDTNSDPDEIDIPIPGNDDAIRSIKFFTQLIA
                     SAMIEGREGVDVMRPSEEDEEESETVSMEEVVAQEVLIDNIENFDGSEEVKKPDVEVK
                     YSQYDEEE"
     gene            2632..3495
                     /gene="tsf"
                     /locus_tag="PK717_03675"
     CDS             2632..3495
                     /gene="tsf"
                     /locus_tag="PK717_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOR46795.1"
                     /translation="MENTSELIKALRERTGAGIMECKKALTETKGDLEKAIDLLRQKG
                     IAKAAKKAERETSEGRIGTATSSDRKRVSFVKLSCETDFVAKNELFADLVNNIAKAVL
                     DANTDDINKILALPYPGGKGSIDEQIKELIGKIGENMQLQEATIIDAQKGIIDVYAHL
                     GNKLAVMVEIHSDKQDVEELTKLAHELAMQIAIDNPDYVCREEVPTDVVDREREVIKA
                     SPELASKPDAVKEKIVDGKLGAFFGTCCLLELPYMRDGSIKIKDIVSETSKKLGAKVD
                     VICFKRASIGK"
     gene            3498..4241
                     /gene="pyrH"
                     /locus_tag="PK717_03680"
     CDS             3498..4241
                     /gene="pyrH"
                     /locus_tag="PK717_03680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOR46796.1"
                     /translation="MGDPAHSQLKPKYKRILLKLSGEALLGEQPYGIDIKYINDVSKI
                     LIDLHNNGVEVAIVVGGGNIWRGSAGVALGMDQVQADYMGMLATVMNGLALQTVIEKL
                     GVPTRLQTAIAMHEIAEPFIQRRAIRHLEKGRIVIFAAGTGNPFFTSDTAAALRAAEV
                     RADCLLKATKTDGIYTDDPAKKRDDIKKLTNISYTKILADNLRVMDGAAVSICRDNKI
                     PIIVFSIAEPENIYKVVMGEEIGSIVEGG"
     gene            4244..4792
                     /gene="frr"
                     /locus_tag="PK717_03685"
     CDS             4244..4792
                     /gene="frr"
                     /locus_tag="PK717_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOR46797.1"
                     /translation="MDTKTLQDHMEKTIGSLQQNFVGLRAGRVTPAIVAKVMVNAYGS
                     ELPLPQAGNIGVPDGRTMIIEPWDKSLIKEIVTALQKANLGANPTSDGNLIRLPFPPL
                     SQERRQELAKMVKKYGEDAKVAIRNIRRDAIEQVKKAEKSKEISEDERFKREEEIQKT
                     TDKYSKKVDELVVQKEKEILES"
     gene            4789..5493
                     /locus_tag="PK717_03690"
     CDS             4789..5493
                     /locus_tag="PK717_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOR46798.1"
                     /translation="MTRTKIPRHIAIIMDGNGRWATMRGLPRIAGHKQGVVSVRKTIE
                     KAASIGIEVLSLYCFSTENWSRPSEEVQFLMNTFLETLYEERPRMLANNIRFMMIGDR
                     TSLSPDLVKTTENLENETKGNTGLKLVLAINYGGRSELLRAIKGIASDGVPPDKITEE
                     TVRERLYTVELPDPDLIIRTSGEMRLSNYMIWQSAYSELLFMKKLWPDFRERDLMSAI
                     AVYSRRQRRFGGLGGK"
     gene            5490..>5778
                     /locus_tag="PK717_03695"
     CDS             5490..>5778
                     /locus_tag="PK717_03695"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOR46799.1"
                     /translation="MNQKIQRVVTAVVLVPLAIGLTYLSGWFFLCAVILLSSMAIVEM
                     SKMARKTAKSPQIVPLLVVNLAIQIMAYYRSWYLVLGTVFLFLVFLYLVEIF"
BASE COUNT         1788 a         1194 c         1454 g         1342 t
ORIGIN      
        1 agaatgggca gatcaatgat gaaaaaattg atgaaaaaaa caaacgtggc ctcacttagc
       61 gaaatgcttg cgatggcaag agaccttgac gtaaagatgt gggcgtgcaa gatgagcatg
      121 gatgtcatgg gtgtggcaaa agaagacttg ataccagaag tggacaagat tgttggtgtt
      181 gccgcatacg cagcagaagc acaacaatcc aaaatcaatc ttttcatctg aggaaggagc
      241 cataaatgaa cttcgatgtt gaactaaata cgcttggaat gcaatgcccg gtgccgatag
      301 tcaagacggc aaagaagatg aaagaactac aaattgggca gatcctgaaa gtgatctctg
      361 atgatgaggg cataaaagag gacatgccgg catggtgcaa gacaaccggc aatgaattcc
      421 ttggccttga ggaatcaggt ggccagtaca ctgtttacgt caagaaggtg gtagggtgaa
      481 attagggatc gttgttctta ctacgccgta cacttttcag aacatggaca cagccatttc
      541 acttgcaggt gctgcgcttg aaaagggcca ccaggccatg atctttttgt acatggacgg
      601 tgttatagcc gcatcaaacc tcatcaagcc tggaaatgac aggagcatcc cgataaagat
      661 gcagtcactt attgaaaagg gtgtcaaaat aacgccttgt ggtgtttgtt gtaattacag
      721 gggcgttcgc caggcggaca gccagccagg catcaagcat gctggcattg cggcccttgg
      781 tcaactcgtg agcgagtgcg atagggtttt gacattgggg ttctaaaaga tggcaaaaaa
      841 agttgcaatc gttgtttcaa aagcgccata tggctcagca atcatgggtg aggcattcag
      901 ggcggccatg ggtcttccct ctgttggttt ggaaaccaac gttgttcttg agggagacgc
      961 agtgttcgca cttctaaaag accaaaagcc gaaagaagct ctggatttct ggagccttca
     1021 tgatgcgtgg ctgcaatttg aggactatgg gttcagcctt tttgttcaca agccttcggt
     1081 tgaagccagg ggattgacct gtgaacagct ttctcattgc cagacactgg atgattccga
     1141 attcaaaaag atgctccgcg acatggatgc gatattgagg ttctgacatg gaaaagaaaa
     1201 tgtttataat gctgacaaaa tctccatacg ggtgtgaaga aggcaaaata aggatttcaa
     1261 acgcgcttgc cggagatgtg gtagtgtttg cccaggattc ggtctatgcg gcaacaaatc
     1321 cgccaggcga gcttgcagaa ttggtcaagt ccaaaatgtc agaaggaata aagttttatg
     1381 cctctgggcc tgattgcaaa gccagaggtg ttgagccaaa atttggtata aaacaaattg
     1441 gttatcctga aatgattgat ctgataatgg agcgccagct ctcgtattag agtgacaggt
     1501 ggaacagggc aaagctgaac tcaataaggt tttgccctgt ataacacaat ttccgaaaac
     1561 cacttcttaa gaaagaaaca gttatttttt tatttgtcct gataggcatt catttttttg
     1621 gcaatatcca ggccccatgt ttttgcctgt tttttatttg acaaaacaca gtggcatcta
     1681 taaactggct tagttacaaa acgcacatcg cgaaggcttt cggtgaaagt aaaaacaagt
     1741 cgcgatggag gcaaaaccga aaggagcatc aatgagcgta gtaaacatga aacacctgct
     1801 tgaggcagga gtgcacttcg gccaccagac aaagaggtgg aaccccaaaa tgcgtccatt
     1861 tatttttacg gcacgcaatg gaattcacat tattgatctt agaatcacag ctgaaaaatg
     1921 cgagcaggcg tatgccacag tgaggcagat tgcccaatca ggcggaaaca ttgtttttgt
     1981 cggcacaaag aaacaggccc aggacatagt aaaagacgag gcaaaaaggt gtggggccca
     2041 ttatgtgaac cagcgctggt ttggcggaac actcaccaat ttcatcacaa tccataccag
     2101 gattgaaaaa ctccgcgagc ttgaagaaaa agaagtcacc ggcgagctga agcgtcttcc
     2161 aatcaaggaa tatatgggct acaagaagga aagagagaaa ctccagaagt tcctcgatgg
     2221 acttcgcaat atggacactc ttccgcaggc catttttgtc actgataccc acaaggaccg
     2281 tgccgcaata atcgaagccc gcaagcagaa tattaccgta atagccattg tggacaccaa
     2341 ctctgatcct gatgaaattg acatcccgat cccaggcaat gacgatgcca tccgttcaat
     2401 aaaattcttc acccagctga ttgcaagcgc catgatcgaa ggacgcgagg gtgttgatgt
     2461 aatgagacca tcagaggaag acgaagaaga gagcgaaacg gtttcgatgg aagaagttgt
     2521 tgcacaggaa gtcttgatag acaatataga gaatttcgat ggctcggaag aagtcaaaaa
     2581 acctgatgtc gaagtcaaat attcccaata tgacgaggag gaatagccca gatggaaaat
     2641 acttctgaac ttataaaagc actaagagag cgcactggcg ctggaattat ggagtgcaag
     2701 aaggccctca ctgaaacaaa aggcgatctt gaaaaggcaa ttgaccttct caggcagaaa
     2761 ggcattgcaa aggccgcaaa gaaggccgag agggaaacct ctgaaggcag gattggaact
     2821 gcaacctctt ctgacaggaa gcgcgtttca ttcgtaaagc tttcatgtga aacagatttt
     2881 gttgccaaaa acgagctttt tgcagacctg gtaaacaata tcgcaaaggc tgttcttgat
     2941 gcaaacactg atgacatcaa caaaatcctt gctcttccat acccaggtgg caagggcagc
     3001 attgatgagc agataaaaga actgatcggc aagatcggtg aaaatatgca gctgcaggaa
     3061 gcaacgatca tcgatgctca aaaaggcatc attgatgtct atgctcacct tggcaacaag
     3121 cttgccgtaa tggtcgagat tcattctgac aaacaggacg tggaggagtt gacaaaactt
     3181 gcccacgagc tggcgatgca gatcgcaatt gacaacccag actatgtttg cagggaagaa
     3241 gtcccgacag acgttgttga cagggaacgt gaagttataa aagccagccc agaacttgca
     3301 tcaaagcctg atgcagtaaa ggaaaaaatc gtggacggaa agcttggcgc tttcttcgga
     3361 acatgctgcc tgcttgaact cccatacatg cgtgatggta gcatcaagat caaggacatt
     3421 gtttccgaaa cttccaagaa acttggcgca aaagtcgacg tcatttgctt caagagggcc
     3481 tcaatcggta aataaccatg ggagacccag cgcacagtca gcttaagccg aaatacaaaa
     3541 ggattctgct aaagctatcc ggcgaagcgc ttttaggcga acagccctat ggcattgata
     3601 taaaatatat caatgatgta tcaaaaatac ttatcgacct ccacaacaac ggtgtggagg
     3661 tcgctattgt agttgggggc ggaaatatct ggaggggttc agctggtgtt gcgcttggaa
     3721 tggaccaggt tcaggccgac tatatgggca tgcttgcaac ggtcatgaat ggccttgcac
     3781 tccagacagt catagaaaaa cttggtgtac ccacaagatt gcaaactgca atagccatgc
     3841 atgagattgc agagcctttc atccagagga gggcgatcag gcaccttgaa aagggcagaa
     3901 tcgtcatctt tgcggcaggg accggaaatc cgttcttcac atcggacact gccgcagctt
     3961 tgagggcggc ggaggttcga gctgattgcc ttctcaaagc aacgaaaact gatggcatat
     4021 acactgatga tccagccaaa aaaagggacg acatcaagaa actcacaaat atatcctaca
     4081 cgaagatctt ggccgacaac ctgagggtca tggatggtgc agccgtatca atttgcaggg
     4141 acaataaaat tccaataatt gtgttttcta tagctgaacc tgaaaatatc tacaaagttg
     4201 ttatgggtga agaaataggt tcaatagtgg aagggggcta aatatggaca ccaaaacatt
     4261 gcaggatcac atggagaaaa cgattggttc gcttcagcaa aattttgtgg ggctgagggc
     4321 tggaagggta acgcctgcaa tagtggccaa agtcatggta aatgcctatg gttccgaact
     4381 tccacttcca caggctggca acattggcgt tccagatgga agaaccatga tcatcgagcc
     4441 gtgggacaaa tcccttatta aagagatagt gaccgcatta cagaaggcaa atctgggtgc
     4501 aaacccaaca tctgatggca atcttataag gctgccattt cctccgcttt cgcaagaaag
     4561 aaggcaggag ctcgccaaga tggtcaaaaa atatggcgag gacgccaagg tggcgatccg
     4621 caacatcaga agggatgcca tagagcaggt caaaaaagca gagaagagca aagaaatctc
     4681 tgaggacgaa agattcaaaa gggaagagga aatccagaaa accaccgaca aatactcaaa
     4741 aaaggtggat gagctggtgg tccaaaagga aaaggaaatc ctagaaagtt gacaagaacc
     4801 aaaatcccaa ggcacatagc catcataatg gatggcaatg gtcggtgggc gaccatgcgt
     4861 ggtctcccga ggattgcggg ccataagcag ggtgttgttt ctgtcagaaa aacaatagaa
     4921 aaagctgctt caattggaat tgaggtttta tcactctatt gtttttcaac tgaaaattgg
     4981 tcaaggccca gcgaagaagt ccagtttctc atgaacacat ttttggagac cctctacgaa
     5041 gagagaccca gaatgcttgc aaacaatatc cggttcatga tgattggcga caggacgtca
     5101 ctttctcctg atctggtaaa aaccaccgaa aacctcgaaa acgaaacaaa aggcaatact
     5161 ggtctgaaac ttgttcttgc cataaattat ggcggcagga gcgagctttt aagggcaatc
     5221 aaggggattg catctgatgg tgttcctcca gataaaatta ctgaagaaac tgtaagagag
     5281 aggctctaca cagtagagtt gcccgaccct gaccttataa tcaggacttc aggcgagatg
     5341 aggctttcaa actacatgat atggcaatct gcttactcag agcttctttt tatgaaaaag
     5401 ctctggcctg attttcggga gagagacttg atgagtgcaa ttgccgttta ttccagaaga
     5461 cagagaaggt ttggtggact gggaggcaaa tgaaccagaa aatccaacgt gttgtaacgg
     5521 cagttgttct tgtcccgctg gcaattggcc tgacctacct ttcaggctgg tttttcctgt
     5581 gcgcagtcat cttgttgtcc agcatggcaa ttgtcgagat gtccaaaatg gccagaaaaa
     5641 ctgcaaaatc accccagatt gttcctctcc ttgttgtgaa cctggccata cagataatgg
     5701 cctattacag gtcatggtat ctggttttgg gaacagtttt cttgttcctt gtttttttgt
     5761 atcttgtcga gattttta
//
LOCUS       DAOSRI010000089         5778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12665_length_5778_cov_3.497641, whole genome shotgun sequence.
ACCESSION   DAOSRI010000089 DAOSRI010000000
VERSION     DAOSRI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12665_length_5778_cov_3.497641"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="PK717_03700"
     CDS             complement(<1..2944)
                     /locus_tag="PK717_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR46800.1"
                     /translation="MRKFFALFLTLVFTLTIPAVYGLPQAGAVPFTVLKNTIWSGTVI
                     MPEGFYIAPDATLTLMPGTVVKLGGNIGVKGKIKAIGTQNEPIIFTSLKDDVKEDSNK
                     DGDVSKPSPGDWGNVEFPDNCGICELYQVQFLYGGKGGFGTLKFSSGKGSDDRIRVSH
                     CTISKSASMGAWFANSTSVLRECVITDCQDPKTGAGIWIQEDSNPSISGCTVERCTIG
                     IQIDLSCSPKLLGNIIKNCGTNGIKVITGSLDKEVVWDASMPYVFDKFVIGSKGFLRI
                     QPGVIVKSTGQLGVSGRLEIRGTAEKPVIFTSLTDDSVGGDTNNDQDKTAPQPGDWGG
                     IEFTGEAGACTITHTKFSWGGGQAEKGGGYAILKFGAAGGVDNRVKLYNCTVEHSETA
                     GLYMQASSPSIYNCKITDNKNDKLGACVWLQSRSKPILYNCELTDSEWAIYCDGYSYP
                     KSQKLTISGNKYNNIYFESPLLEEDAVWSADLVHYIPKTVGIKPGVTLHLLPGVIIKY
                     GEMIRVDGKLIAIGTKDKPIYFTSLNDDSVGGDSNGDSGAKDPLPGDNQGIQLTESFG
                     RSVMKNCVVRYGGSGGNGNIYLGQATNTKNEQIIEDTIIEKSLGQAVKVRGGQVDLIR
                     CSLLDCMNKQNGFAIIAEAGAKIRLVDSLIQRCRHLFSITPEVELKTQGNTAKNIGVE
                     LEESFAGIHIYKGRLREDKVWDSEFPYIIEDDFTIDKGANLTVAAGNVIKVVKGAFYV
                     NGSMSCKGTQNNPVYITSFSDDTIAGDTNNNGQLTKPMPGDFDEIEFEGSVTACTFEH
                     TVIRYSGGKTRRMGSVKFGAFAGVDGRVKFINCTIENSNSAGLYMINSSPYIKDCIIR
                     NCKTTDRGHGIYLTGKCNPEIQGVTFQDCDYAVYNNMQGTEAVLGNCYFGDASGPKDE
                     TGNPNGNGLKVFGPVNYNPFAQEPNPTGGAGGKGGELPPYKRPEVSFPSPTGAAVLLS
                     VDPTFVELN"
     gene            3202..3666
                     /locus_tag="PK717_03705"
     CDS             3202..3666
                     /locus_tag="PK717_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOR46801.1"
                     /translation="MENIFWVLENRLAGRPGPDEVPWDLGEIYGAGFRAMLSLENNVD
                     PAEIKNFGFEHLLAPMPDEIPPDNNSISAYKRLLPGAIDFIDVQISKGLPVLVHCHAG
                     MDRTGMAIACFLVRHRGMAPKEALSKVRMTRPVALTSKGYEAAYFAVASSLI"
     gene            complement(3832..4431)
                     /locus_tag="PK717_03710"
     CDS             complement(3832..4431)
                     /locus_tag="PK717_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46802.1"
                     /translation="MKPELKISIADLGKLFAEYCMSQGIIEDFDDNEVSLTEKGIEAL
                     QGAPYYFDLEIGRDEGAIAEDYLESETTFTWYQKNFQPGRRRLPMSDDRQRRSGGNED
                     MLSWRPGISSPQREEWRPPQDRPQQNRYGNQNKKYFKGKQGGQQHQGQQQKPQQHHQH
                     HQQKQDLKPIKMEVDLPPIARTNYNKYGKYNNKPRNNNK"
     gene            5219..>5778
                     /locus_tag="PK717_03715"
     rRNA            5219..>5778
                     /locus_tag="PK717_03715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1392 a         1302 c         1370 g         1714 t
ORIGIN      
        1 catttagctc tacaaaagtg ggatcaacgg aaagaaggac tgcagcacct gttggcgacg
       61 ggaagctgac ttctggcctc ttgtatggtg gcagttctcc accctttccg cctgctccgc
      121 ctgttgggtt tggctcctgg gcaaacgggt tataattgac tggaccaaac actttgagtc
      181 cattcccgtt cgggttgcct gtttcatcct tcggccccga tgcatcacca aagtagcagt
      241 ttccaagaac ggcttctgtt ccctgcatgt tgttgtagac tgcatagtcg cagtcctgga
      301 atgtgacacc ctggatctcc gggttgcatt tgccggtgag gtagatgcca tggcccctgt
      361 cggtggtttt gcaattcctg atgatgcagt cttttatgta gggtgaagaa tttatcatgt
      421 agaggccggc actgttactg ttttctatgg tgcagttgat gaattttacc cttccgtcaa
      481 caccggcgaa tgcaccaaat ttgaccgagc ccatgcgcct ggtttttcct ccgctgtacc
      541 tgatgacagt gtgctcgaag gtgcatgccg ttactgaccc ttcaaactcg atctcgtcaa
      601 aatcgccagg cattggtttt gtcagttgcc cgttgttgtt tgtgtcacca gcaatggtgt
      661 cgtcggagaa tgatgttatg tagacaggat tgttttgcgt tcccttgcag gacatcgaac
      721 cgttcacata gaaggcgccc ttaacgacct tgatgacgtt tccggccgca acagtgaggt
      781 tggccccctt gtctatcgtg aagtcgtcct cgatgatgta cgggaattca gaatcccaga
      841 ccttgtcttc ccttagccta cccttgtaga tgtggatccc ggcaaaactc tcttccagct
      901 cgacaccaat gttttttgcc gtgttgcctt gcgttttcag ctcgacttca ggggtgatac
      961 tgaagaggtg tctgcagcgc tgaatgagtg agtcaacgag tcttatcttt gcgcctgcct
     1021 ctgcaataat tgcgaaacca ttttgcttgt tcatgcagtc gaggagggag caccttataa
     1081 ggtcgacctg accacccctt actttcactg cttgaccaag cgatttctcg attatggtgt
     1141 cttcaattat ctgctcgttt tttgtgttgg ttgcctgtcc gaggtatatg tttccgttgc
     1201 cgcccgaacc gccatacctt acgacgcaat ttttcattac agatctgcca aaagactcgg
     1261 tcaattgtat gccctggttg tcgccgggga gtggatcctt ggcgccacta tcaccgttgc
     1321 tgtcgccacc cacgctgtca tcgtttaatg aggtgaaata tattggcttg tcctttgtgc
     1381 cgattgctat tagcttgccg tcaactctta tcatttcgcc gtacttgatg atgacgccgg
     1441 ggaggaggtg gagtgtcaca cctggtttga tgccgaccgt ttttgggatg tagtggacaa
     1501 gatcggcgct ccataccgca tcttcctcaa gaagcgggct ctcaaaatag atgttgttgt
     1561 atttgttgcc ggagatggtg agtttttgcg attttgggta tgagtacccg tcgcaatata
     1621 ttgcccattc ggaatctgtg agctcacagt tgtagaggat tggcttgctc ctgctctgaa
     1681 gccagacgca tgcccccagc ttatcgttct tgttgtcggt tatcttgcag ttgtagatgc
     1741 tcgggcttga tgcctgcatg taaagaccgg cagtttccga gtgttcaacg gtgcaattat
     1801 acagtttgac cctgttgtca acgccgcctg cggcaccaaa cttcaggatg gcataaccgc
     1861 cacctttttc agcctggcca ccaccccatg aaaattttgt gtgtgttatc gtgcatgctc
     1921 cggcttcgcc agtgaactcg attccacccc agtcacccgg ctgtggcgca gttttgtcct
     1981 ggtcattgtt tgtgtcaccg ccaacactgt catcggtaag agatgtgaaa atgactggtt
     2041 tttctgcggt tcccctgatt tccagacgac cagagacccc gagttggccg gtacttttta
     2101 caatgacgcc tggctggatt ctcagaaatc ctttgctgcc gattacaaac ttgtcaaaaa
     2161 catagggcat agaggcgtcc cagactactt ccttgtccag ggaacctgtg ataaccttta
     2221 tcccgtttgt accgcagttt tttatgatgt ttccaaggag ttttggtgag caagaaaggt
     2281 cgatctggat gccaatggtg cacctttcaa ctgtgcaacc ggagattgat gggtttgaat
     2341 cttcctgtat ccagatgcct gcgcctgtct ttgggtcctg gcagtcggtt atgacgcact
     2401 ccctcagcac cgatgtggaa tttgcaaacc atgcgcccat tgaagccgat ttggatattg
     2461 tgcagtggga gactcttatt ctgtcgtcgg agccttttcc ggatgaaaat ttgagtgtcc
     2521 caaaaccacc cttgccacca tagaggaatt gcacctgata aagctcgcag atgccgcagt
     2581 tgtccgggaa ttcaacatta ccccagtcac ctggcgaagg ttttgacacg tcgccatctt
     2641 tgtttgaatc ctctttgaca tcatccttga gcgatgtgaa tatgattggc tcattttgag
     2701 tcccgatggc ttttatcttg ccttttacgc caatattgcc acccagtttg accactgtcc
     2761 ctggcattaa tgtcagggtg gcatcagggg ctatataaaa accttctggc ataatgactg
     2821 ttcctgacca gatggtattt ttcagaacag taaaggggac cgccccggct tgtggaagac
     2881 cataaacggc gggtatcgta agggtaaaca ccaatgtaag aaaaagagca aaaaacttcc
     2941 tcattccaat cctcctttgg ttctctagtt tactcctggt ctcaaagtct tcaagtgaac
     3001 ataaacaggg agttcatcca gaactcttgg aaacagaaac ctgattgcta cgagaaaata
     3061 ttatatcatt atgtaaattg attgtatgta aatatttttt gttcgagttt attcttatgg
     3121 attgtttgct tatagaatga atctaaactt tattagtaga aattaatttc gtattgccag
     3181 tgcgtcgcta ttattattct tatggaaaat attttctggg tgcttgaaaa caggcttgcc
     3241 gggagaccag gccctgatga agtgccgtgg gatttggggg agatatatgg ggctggtttc
     3301 agggcaatgc tctccctgga aaacaatgtt gatccggcag agatcaaaaa ttttggtttt
     3361 gaacatctcc ttgccccaat gcctgacgag ataccgcctg acaacaacag tatttctgca
     3421 tacaaaagac tccttcctgg tgctattgat tttattgacg tacagatttc aaaagggctt
     3481 cccgtgcttg ttcactgtca tgccggaatg gacaggacgg ggatggcaat cgcttgtttt
     3541 ttggtgcggc atcgtggcat ggcccctaag gaggcccttt ccaaagtgag aatgacgaga
     3601 cctgttgcgc ttaccagcaa aggttacgag gcggcttatt ttgctgtggc ctcatcccta
     3661 atctaattgt gttttggttt accatcggta tttacttcaa tgaggattgc cattggcagg
     3721 aacattagat ttgtttggca agatttattt tcatctacag gattactcct taaaaggtaa
     3781 taaagatcaa ttgtcctaat aaattggaag cagatttttt tggcgaaaaa tttatttgtt
     3841 attgtttctt ggtttgttgt tgtatttccc gtatttgttg taatttgtcc tggcgattgg
     3901 tggaagatcg acctccattt ttattggttt gaggtcttgt ttttgttggt ggtgctgatg
     3961 gtgttgctgg ggtttttgtt gctgaccctg gtgttgttgt ccaccctgtt tccccttgaa
     4021 gtattttttg ttttggttgc catacctgtt ttgttggggc ctgtcttgtg gtggtcgcca
     4081 ttcttcgcgc tgtggcgatg agataccagg cctccagctg agcatgtcct cgtttccgcc
     4141 ggaacgcctt tgcctgtcat ctgacattgg tagtcttcgc ctgcctggct ggaaattttt
     4201 ttgataccat gtgaaggtgg tttcagactc aaggtagtct tccgcgatcg ctccctcatc
     4261 tcttccgatt tcgagatcaa agtagtaagg agcgccttgg agtgcctcaa tgcccttctc
     4321 agtgagcgag acttcattgt catcgaaatc ctctatgatt ccctgagaca tacaatattc
     4381 cgcaaagagc tttcccaaat cagcgatact tattttaagt tccggtttca tcgccagaaa
     4441 ttatatttgt tttgtgcctt ttgacaacat aataaatttt gtggaatatg ctgcaatacc
     4501 ataagaacca gcaaagtacg atttaaaaca actgtcatgt gaaaattgga ccattaaaaa
     4561 tgttgttttc atgcgctttg ctagtggcaa tcgagctatt tcttgtcttg gggtattgac
     4621 aaaccagcaa aaatgtataa atacaaactt gtgcttgaaa acatatcttt tgccacaaga
     4681 aattcaataa gaactcaaaa caatcacaaa acatcaaaga acagctctgg ggaatccata
     4741 aaacacactt ttatattgca aatctattgt tttttggcca tctgcccagg agcagctgat
     4801 tgaagaagtt ttgccaagaa ctcccttcca ggagatctca attgaggatt ggcctgtcat
     4861 ttttaccacc ccgactcatg gttaaaacca acaaccccaa aatttgcctc atatcagttt
     4921 ttgattatga gctttcccat gtacgaataa atatagccac agagagtgtt ttaaaacatc
     4981 gaaatatatc gagggaggca tgcctataag ggtatcaaaa gtttaaacct gatgatctgg
     5041 gacaaaacta ggtaacctat aaaataacga taaatactaa ctcctctagc tgtcattgtc
     5101 tcccttaatc aaaggggttg acagggggga gttttttgct aatataggat ttcgttacga
     5161 ctcagttgag tctgtgacgg caagatcagc actttgaaaa ctatgtagga atgcaggtag
     5221 ctaagcgaac aagagcgcac agcggatgcc ttggcacttg agatcgaaga aggacgcagc
     5281 aagcccgcga aagtgatcgg tgaggagcaa acatccatag acccgatcgt gtccgaatgg
     5341 ggcaacccag caacctttca ggttgttatc ccacgatgaa taaatagtcg tgaggaaggt
     5401 aagccggcca actgaaacat cttagtaacc ggtatagaaa aaagcaaata gcgattcccc
     5461 cagtagcggc gagcgaaaag ggaacagcct aaaccgttta tgcatgcggt ttgcagactt
     5521 agtataagcg gtgtagtgag gcaacaattt tggcactgca atgccaaaga gaagttacaa
     5581 aggttctgat taatcgaatg gcgtggaaaa gccaaccaca gaaggtgaca gtcctgtagg
     5641 tgaaagtcag actcttctct gttgtcctcg agtaccacgg ggcacgtgaa atcctgtggg
     5701 aatccgggag gaccatctcc taaggctaaa catctcagtg accgatagtg aacaaagtac
     5761 cgcgagggaa aggtgaaa
//
LOCUS       DAOSRI010000090         5729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12796_length_5729_cov_3.884914, whole genome shotgun sequence.
ACCESSION   DAOSRI010000090 DAOSRI010000000
VERSION     DAOSRI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_5729_cov_3.884914"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PK717_03720"
     CDS             <1..1098
                     /locus_tag="PK717_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOR46803.1"
                     /translation="AGGLPDPTMFPVEQFNECMSHVIKTIPSVVFQYGETPGYKPLRE
                     IVVEMTKKEGIKDCTIDNVCITTASQQGIELVGKIMVDEGDVVIVEGPTYLATLQAWS
                     VYGAKYVTVDLDDEGMKVDELEERLEAMKKVGVRPKMIYTIPTFQNPAGVTMTLARRK
                     KLLELAYKYNVPIIEDNPYGDLRFTGTPTPSLYSLDTKGMVVSLKTFSKILAPGLRLG
                     WIVANKDFIAKICMAKQSADLNSPVITQAAVVEFAKRGYIDQHVPKICEAYGKKRQAM
                     EVAMKQYLPQGVKWTNAEGGMFIWVTLSNNIDTTALFPKAIEHKVAYIVGSAFYPYGG
                     GESSMRLNFSFPSLPQIDEGIKRLGDLIKSI"
     gene            1116..2219
                     /locus_tag="PK717_03725"
     CDS             1116..2219
                     /locus_tag="PK717_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46804.1"
                     /translation="MSKKSYKPVQQAEEAVKSAETKPARATANHGHSQQVKNPFKIIA
                     YTFMSTTLVLLIILTATLFSCSSGGASSSDSSIASVLGKSYVTLGRKEVAANPDDKSQ
                     EITFWTWQDRNNAWGYCTVMDVPNYPSILLIVDANFKIEKLQEMSEFPIAGADKRAEY
                     RDILGRYEGRTLVDFTSLELNVAEGDTRIFREMLRDQVARSLMTMYVKINGDEQFNTL
                     FPQGIKFAKVGTKLNAWSVSDADGKEISSNYYAGRKYAIFTTTSCGSCINTCIGLAQR
                     LITEGSMSPDQISIVFTSAKDKVDYLKNQMKGEHLVYDENMRGFAKSLNLFEGPSMML
                     VNSEGIVIAKEPSQRLSDQVNVDKVLQIFFKEK"
     gene            complement(2540..3547)
                     /locus_tag="PK717_03730"
     CDS             complement(2540..3547)
                     /locus_tag="PK717_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOR46805.1"
                     /translation="MKNKVFIVIASVAALLVVLTCVALVAPENKRDEGANNGAYVVDD
                     AGEHWYESDGALSKNTAKTKAILPASSTQGIGRGAMEQKATDMAATAEYAGSAPSPVP
                     TASIPGNTAKPIGIKQMMKKDGNVSLKADKPQEAANAIISIATKFGGDVLSMQSSGEK
                     TSQYFNITIRVPSQKFDNAMQEVLKLGDKASVTTSSEDVTGEYIDMKSRLDSRTRKRE
                     ALLTMLTKADKIGDLLSIHDRIADVEQEIESIKGQMQYLEGMTSYSRITVDISPKDGL
                     ISTDSPSGFVDGLGKIWVAFQKAILWLLMIIAVLIPFGIITLLVYFIVKASRKRPGAQ
                     S"
     gene            complement(3594..4235)
                     /locus_tag="PK717_03735"
     CDS             complement(3594..4235)
                     /locus_tag="PK717_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46806.1"
                     /translation="MKKIDDRELTGLLRGLHQEPPPVFAFDVMQKVRLHQPGRTKLFW
                     TLGIGIGMAAVATLAIAFSIPFGGQPQNNLEIAQNIPTFDNRGFAAKSAPPPAPVQDE
                     AAGTAVMASYGNDGPCDTAQQYQEGFTIIRIKYQDPYAMILSLKSQLVSYSPTEFTDN
                     GLPSLALIINGENIIPIFNAIVTASQGRALYQIAKLQDTPPDGRTRVLISLSN"
     gene            complement(4260..4817)
                     /locus_tag="PK717_03740"
     CDS             complement(4260..4817)
                     /locus_tag="PK717_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR46807.1"
                     /translation="MSPERVLEIAECIRKGDREPFSEIIDEYQGFVYSFSLRLVRGNR
                     DSAYDLSSEVFLRVLTQISKYKPEYPFKSWLGRVTYTTGLNYIRKKRETIQIEDLQED
                     VPFQVPDDSPTPEEEVIQKFTKEDVSKALNNLKPDFRAIITLCDIEGVSYEEAASILG
                     IPTGTVRSRLSRARSALKESLDGTL"
     gene            4914..>5729
                     /locus_tag="PK717_03745"
     CDS             4914..>5729
                     /locus_tag="PK717_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46808.1"
                     /translation="MEDNIENKEEITSDELWRMANLDQKARLLVQMDKSVKPIWIASF
                     FLVALAILLYFVVPSPDPAISALSINKSTVACKSDSKAFLIRDGKPIGNFEVEPQTNI
                     IAIGDTRVCLGTRTTGKLTEYSFDGKIIKKHQIDENVFNIVYEGDELWVVTASSLTKI
                     SDGKKIIAPEYSVAQNVSFSPFPSITDIDGIWIYVDGEWLSFKSKDPIVCAFAGETEI
                     SLVGEDGQVQVFSFEDRKILKKEFVSWVKRPVVMWDGAIFCADGGELVSGQITD"
BASE COUNT         1527 a         1234 c         1353 g         1615 t
ORIGIN      
        1 gcgggtggtc ttccagatcc gaccatgttc ccggttgagc aattcaatga gtgcatgtcg
       61 catgtcatca agacaatccc gagcgttgtt ttccagtatg gtgaaacacc aggctacaaa
      121 ccgcttcgtg agattgttgt tgagatgacg aaaaaagaag gtataaaaga ttgcacaata
      181 gacaatgttt gcattacaac agcttcccag cagggcattg agcttgtcgg aaaaatcatg
      241 gttgatgaag gtgatgttgt catagtcgaa ggaccaactt accttgccac attgcaggca
      301 tggagtgttt atggtgcgaa gtatgttact gtagacctcg atgatgaagg catgaaggtt
      361 gatgaactgg aagagaggct tgaagcaatg aaaaaagttg gtgtcaggcc gaagatgata
      421 tacacaatcc caaccttcca gaacccggcc ggcgttacca tgacccttgc cagaaggaaa
      481 aaactccttg agttggcata caagtacaat gttccgataa ttgaggataa tccttatggt
      541 gatctcagat tcacaggcac accgacccca tcgctctact cactcgacac aaagggaatg
      601 gttgtatcgc tcaagacctt ctccaaaatc cttgcaccag gactcaggct tggctggatc
      661 gttgcaaaca aagattttat cgcaaaaatt tgcatggcaa aacagtcagc agacctcaac
      721 tcgccagtta taacccaagc tgcagttgta gaatttgcaa agagaggcta tatagaccag
      781 catgttccaa aaatctgcga agcttatggc aaaaagagac aggcgatgga agtggccatg
      841 aagcagtatc tcccgcaggg tgtaaagtgg acaaatgctg agggtggaat gttcatctgg
      901 gttacacttt caaataatat cgatacaaca gcccttttcc cgaaagccat cgagcataag
      961 gtcgcctata ttgtgggatc ggccttctac ccatacggtg gtggagaaag ctccatgagg
     1021 ctgaacttct cattcccgtc attgccgcag attgatgagg gaatcaagag gctcggagac
     1081 ttgataaagt caatttaata taattggagg agacagtgtc caaaaaaagc tacaaaccag
     1141 tgcagcaagc agaagaagcc gtcaaatcag cagagacaaa gccagcaaga gcaaccgcaa
     1201 atcacggcca cagtcagcag gttaaaaacc cgttcaagat cattgcatac acattcatgt
     1261 ccacgacctt ggttctcctg atcatcctga ctgcaacgct cttttcgtgt tcttctggcg
     1321 gggcctcatc ctctgattca agcattgcat cggtgcttgg taagtcttat gtaacactgg
     1381 gcagaaaaga agttgctgca aacccagatg acaagagcca ggaaatcact ttttggacat
     1441 ggcaggacag aaacaatgcc tggggatatt gcactgttat ggacgtcccg aattacccat
     1501 caatcctcct catagtggat gcaaacttca agattgaaaa attgcaggaa atgtcagagt
     1561 tcccgatagc cggcgcagac aagagggctg agtacaggga catccttggc agatacgaag
     1621 gaagaacact cgttgatttt acctccctag agctcaatgt tgccgagggt gatacgagga
     1681 ttttcaggga gatgctcagg gaccaggttg caaggtcact gatgacaatg tatgtgaaga
     1741 tcaacgggga cgagcaattc aacaccctgt ttccacaggg catcaagttt gccaaggttg
     1801 gcacgaagct caacgcctgg tcagtttctg atgcagatgg taaggaaatt tccagcaatt
     1861 attatgctgg aaggaagtat gcaatattta caaccacttc gtgcggctca tgcataaaca
     1921 cctgcattgg tcttgcacag agacttatta ccgaaggcag catgtctcct gatcagattt
     1981 caatcgtttt cacatcggca aaagacaagg ttgactacct gaagaaccag atgaagggcg
     2041 agcatcttgt gtatgatgaa aacatgaggg gatttgcaaa atccctgaac ctttttgaag
     2101 gtccttcgat gatgcttgtc aactcagaag gtattgttat tgccaaggaa ccgagccaga
     2161 ggctctctga ccaggtcaat gttgacaagg ttctccagat attcttcaaa gaaaaataga
     2221 tcgtaacaga actggattat aaaagcccac ccagaagggt gggctttttg tttgtggggg
     2281 tgtttatatc tttaaagacg tacagaatgc ccttattttt gatttctagt ttttggtcga
     2341 gaaattgtga gtgtaagaca ccaaaatcaa aaaattggcc ctccagatgg cacataatga
     2401 taatctgaag gactgggtat caagaaaatt gtcatattat ggctgtttgt tgtaagaaat
     2461 aaaaaagacc ctgttcattt ttgaacaggg tcttttttat tacaaacaca atcttggatt
     2521 gtttcgggca attttttggt cagctctgtg cgcctggtct cttgcggctg gccttcacta
     2581 tgaaatagac gagaagggta ataattccga atggaatcaa aactgcaatg atcatcagga
     2641 gccacagtat tgctttctgg aatgctaccc atatttttcc caggccatcc acaaaacctg
     2701 aaggactatc ggttgaaatg agtccatctt ttggcgagat atcaacggtt attcttgagt
     2761 atgaggtcat tccttcgagg tattgcatct gaccctttat gctctcaatt tcctgttcca
     2821 catcggcaat tctgtcgtga atcgatagca gatcgcctat cttgtcagct tttgtgagca
     2881 ttgtaagcag ggcttccctt ttccttgtcc tgctgtccaa tcttgatttc atgtcaatgt
     2941 attctccagt cacgtcttcg gatgatgttg tgactgaggc cttgtcaccg agtttcagca
     3001 cttcctgcat tgcgttgtca aacttctggg atggcacccg tatggttatg ttgaaatatt
     3061 gtgaagtttt ctccccggaa gattgcatgc tgagcacatc cccaccgaac ttggtggcta
     3121 tgctgatgat ggcattcgca gcttcctgcg gtttatcggc cttgagggac acattgccat
     3181 cttttttcat catctgtttg atgccaatgg gttttgctgt gttgccgggt atcgatgcgg
     3241 ttggcactgg tgaaggagcg cttccggcgt attctgccgt ggcggccatg tcggtggcct
     3301 tctgttccat tgctcctctg cctatgccct gggtggatga tgccggcaat attgcttttg
     3361 tttttgctgt gttttttgaa agggcaccat ccgactcata ccaatgttcc cctgcatcgt
     3421 cgacaacata agcaccattg tttgcacctt catccctctt gttttctgga gctacaagag
     3481 caacacatgt gagaacaaca agcaatgccg caactgaagc aattacaata aataccttgt
     3541 ttttcatgat tttctcccaa aaagttgaat tttttgtttc tacaaaatgt tttttaatta
     3601 ctgagactta tcagcactct tgttctgcca tctgggggtg tgtcctggag ttttgcaatc
     3661 tggtagagtg cccttccttg tgaagctgtg actatggcgt tgaaaattgg gatgatgttt
     3721 tcaccattta taatcaatgc cagggacggg agaccattgt cggtaaactc ggttggcgaa
     3781 tatgaaacaa gctgagattt gagggagagg atcatggcgt aagggtcctg atattttatc
     3841 ctgatgattg tgaacccctc ctggtattgc tgtgcagtgt cgcatggtcc atcatttccg
     3901 taagatgcca tcactgctgt tcctgctgcc tcgtcctgga caggtgccgg tggtggtgcg
     3961 ctctttgcgg caaaaccgcg attatcaaat gttggtatat tttgggctat ctctaggttg
     4021 ttttggggtt gtcctccaaa aggaatagag aaggcaattg ccagcgtggc cacggccgcc
     4081 atgcctatcc cgattcccag tgtccagaag agttttgttc taccaggttg atggagtctg
     4141 actttttgca tgacgtcaaa agcgaacact gggggtggtt cctggtggag tccccttaaa
     4201 agtccggtta gctctctgtc gtcaatcttt ttcatttcat cctccataca aatctgcggt
     4261 cataatgttc catcaaggga ctccttgagg gccgatcttg ccctggacag tcttgatctg
     4321 acagttccag ttggaatgcc aagtatcgat gcggcctctt cgtaggacac cccttcgatg
     4381 tcgcaaagcg ttatgattgc cctgaagtcc ggtttcaagt tgtttagagc ttttgagacg
     4441 tcctctttgg tgaatttttg tatcacttct tcttcaggtg tcggtgaatc gtctggcacc
     4501 tggaaaggaa cgtcttcctg caaatcttct atctgtatgg tttcgcgttt ttttctgatg
     4561 tagttgagtc cagttgtgta tgtgaccctt ccaagccatg atttaaaggg atattccggc
     4621 ttgtatttgg aaatctgggt gagcaccctc aaaaaaacct cggaagagag atcgtaagcg
     4681 gaatccctgt ttcccctgac cagcctcagc gaaaaactgt atacaaaacc ctgatactca
     4741 tcaataattt cggagaaggg ttccctgtct ccctttctga tgcactcggc tatttccaaa
     4801 acccgctcgg gactcacgca agagattata ttaaatggtt aacaaagggc aagcaaaaat
     4861 cagagaggct ttttcaaatt gattttggcc tggcagacaa tacaatatct gtcatggaag
     4921 ataacataga aaataaagaa gagattacct cggatgaact gtggagaatg gccaatcttg
     4981 accagaaagc aaggcttctg gtgcagatgg acaaatcagt aaaaccgatc tggattgctt
     5041 cattttttct ggttgccctt gcgatccttc tctattttgt tgttccctcc ccggaccctg
     5101 ccatttcagc actctctatc aataaaagca ctgtcgcatg caagtctgat agcaaagctt
     5161 ttttgataag ggacggcaag cccattggaa attttgaggt agaaccgcaa accaacatta
     5221 ttgcaattgg tgataccaga gtgtgtcttg gaacgaggac cacaggcaaa cttaccgagt
     5281 actcttttga tggaaaaata ataaaaaaac accagattga tgaaaacgtt ttcaatattg
     5341 tctatgaagg ggatgaactc tgggtagtaa ctgcctcttc acttacaaaa atttcggacg
     5401 gcaaaaaaat cattgctcct gaatatagtg tggcccagaa tgtgtctttc tcaccgttcc
     5461 cttccattac cgatatagat ggaatctgga tttatgttga tggggaatgg ctgtctttca
     5521 aatcaaagga tccgattgtc tgtgcttttg ctggcgaaac tgaaatttca ctcgttggag
     5581 aggatggcca ggtacaagtg ttttcttttg aagaccgcaa gattttgaaa aaagaatttg
     5641 tgtcctgggt caagagacca gttgttatgt gggatggtgc cattttttgt gcagatggcg
     5701 gtgaactggt ttctggtcaa ataaccgac
//
LOCUS       DAOSRI010000091         5727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12800_length_5727_cov_3.755465, whole genome shotgun sequence.
ACCESSION   DAOSRI010000091 DAOSRI010000000
VERSION     DAOSRI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12800_length_5727_cov_3.755465"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1930)
                     /locus_tag="PK717_03750"
     CDS             complement(98..1930)
                     /locus_tag="PK717_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46809.1"
                     /translation="MKETKKWSALLFDPTGKLLGRKDFTVDPEKALEEEVMSSIQFPT
                     ALLSMKSGGFYAIFVSYDALFVEKWDEQFELTDKKMVQLEGIYVNSVYKTSSDTFYMA
                     ITAGFMEEGLSENKFKICKVGPDFKTQLMVPFIVRQSNRLVSPYFMEFSEDGDLYIGG
                     EQKCAVYDRFGTRKDDFVISSGDEKTAFKPVMLKFDSGKIVALSIDYFESNIIISDKS
                     GNLLRKISLSGENGVYPYAIEPNLSQNEIYILDGMGKIHIISSYLDQDKNPAGFEPKK
                     SVSIVGYGKGLAQAPVAMRYFNGKLYVLDFYGQKICCYDKDLNFLFEFGGLGEGTGLL
                     MDATSLCADNNGNIWVGDMSKSKLVIYSENGGFLGEIGKEGFYASPGSIEAYSNDSFS
                     WMSPLDFAVGKGGLAVFDYGHDRILMWNSKNESTTMSITPDDLKVTIFPTQKIFKYTF
                     EITNTGSGNLEGTISSETGGVMVAQPKIIKNIHEVVLECDFSNTDIPKSINVLIQTNV
                     GTKKINIPILIVPLDVLLVVGEPVCGNANGVFRLAMLPYSKNDKIIVSTKDLERMFGL
                     KGIIAKDNNSAYFTYGQRRIGFVNGSSIADLQIGEDHFNVNLGV"
     gene            complement(2105..>5727)
                     /locus_tag="PK717_03755"
     CDS             complement(2105..>5727)
                     /locus_tag="PK717_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOR46810.1"
                     /translation="DYKGESAVQSFNPLVCGWFRKNAPDFTAGLLLDDIPFLRLKPVR
                     NLKEANDVVDKIVRYRKSIKPEWFKKIVLSGGDPFSGGFLGELECQSLVDNGYLDGFG
                     ISKKYKTRDSFDSESLLKAFTEDNGLVYCVSHGAGNAIITEPGKITNTDIMGLTKRDK
                     LPVLVSVACTNGMFDNSVVGVEFKDTPEYKDISFAQACLFSQGGPVAYFGGSRLNYAG
                     INWTIEGGVAKALPFDEMDRLTTEVVNAYNNWATTLGELYSQALKKYVGVAKEGFFTT
                     SKSLFCFTFLGDPTIKLPQNSGGAPHRMPDIEVLDCPMTSDGLSIPIMSVVEPNQVLV
                     KTDFEWIESRKIDLETAKSQPAQKLTNPTNKTFKVPFSHNTKSLWQARIELPNSSEIW
                     IYYITRAKYDLKAVSNRLFYTDKPSKRQNFSFQVINEGYKNLQNIVAQLKIDGVTRKT
                     KKIGSLEINSLKQLNFMVEGLEEGEHTVNFEVKSDFKDDFPKDNVFEKQLVLTKSETA
                     KALCLISYLFNASTAKKVFGIDSYESSVKDFKKIPTEIVTYGDDSYMSLLFGFGYQNF
                     AELGADTVIMASPNFANPYDSKFAQGLLSFVENGGQAIGYACLGSESGGSNYTAYAKV
                     FGFKQSLEYGLSEDAFTFDVPVDPANDFFQGIDGQMLARAIMSNTIPAQSWDECLDGA
                     KIVLKSRDGKNIIATSGGNSVYFSTLYENTNPEHFRFLYNLLTYSHRPQPDVSLYSGS
                     ISCDPPVALTSSRVKVSVSVVNSGNVASKEARLVLEPFGFSKTVPQIEKGGKTNLEFE
                     IETPDSPGILELGANITMQGEVNEKNNDSTYRLQVLPKGREGSLEAISNLNISDFMIL
                     PDKPFYVTGKAQKSALINCQGSLARVSDKGDFKVYVKPSGQGLELGVTTIEGFSSSKK
                     INCTFATQKNVAGTIGKNYFIQNQTYTKDLKTQPFFQRNNETYVNFTVVGKTLGLDVT
                     TDGDRWSIGNSSFTMSGTLETGNAKIEMGGAQKDVQSQQPIMMLDSGLCVPASFLSQV
                     SFKIEQDNKPGSFLVLFPNNVKLPETPVEQVFSNGFAQPETKELRIPKETDYGPPVLL
                     SYGALDGEVKDVMSLSPTKKGLYLLTSRGVELWTAEGAFSKCIGFPPTLVADVGGSWY
                     SFLYNNSNSFDGRTPKIAFAANDSNNFAFATQDCLAIYDETLKLIKKYKFNDPIYLDV
                     GI"
BASE COUNT         1597 a         1274 c         1088 g         1768 t
ORIGIN      
        1 tctttatgaa attgccttct ggaataactt tgcaacttaa gaatgctgca attgtttcaa
       61 caggcacagt aaggccaccg tcaataagtt ttgtgactta cacacccaaa ttaacattaa
      121 agtgatcctc accaatctgg agatcagcga tactggagcc attcacaaaa ccgatccttc
      181 tctgcccata tgtgaagtat gcactgttgt tgtcttttgc aataattccc ttcaaaccaa
      241 acatcctctc caagtctttt gtagatacaa tgattttatc attttttgaa taagggagca
      301 tcgcaagtct aaaaacacca tttgcattgc cgcaaaccgg ctcgcccacc accaacaaga
      361 catcaagcgg aacaatcaat atcggtatat ttatcttttt ggttccaacg ttggtttgga
      421 tcaaaacgtt aatgctcttt ggtatgtctg tatttgagaa atcacattca agcacaactt
      481 catggatatt ttttattatc ttaggctggg caaccattac tcctccggtt tcagacgata
      541 ttgtgccctc aagattgcca ctaccagtat tcgtgatctc aaatgtatat ttgaaaatct
      601 tttgtgtcgg gaaaatggtc accttaagat catcaggggt aatggacatc gttgtggact
      661 catttttaga attccacatg agtatcctat catgaccata atcaaaaacg gcaagccctc
      721 cttttccaac tgcgaaatca agtggtgaca tccaggagaa ggagtcattg ctataagcct
      781 caattgagcc cggagaagcg taaaaaccct ctttcccaat ttcgccgagg aaaccaccat
      841 tttcagagta gatgacaagt ttcgacttcg acatgtctcc aacccagata ttcccattgt
      901 tgtctgcaca caatgatgtg gcgtccataa gcagaccagt tccttcacca agcccgccaa
      961 actcaaagag gaaattgaga tctttgtcat agcaacatat cttttgacca taaaaatcta
     1021 aaacatagag tttgccatta aaatatctca ttgcaacagg agcctgagct aaacctttgc
     1081 catagccaac tattgaaacc gatttcttgg gttcaaatcc cgctggattt ttgtcctgat
     1141 caaggtaaga agatattatg tggatcttgc ccattccatc caagatatat atctcattct
     1201 gcgacagatt cggctcaatg gcataaggat aaactccgtt ttcacccgac aaggatattt
     1261 ttctgaggag atttccgctc ttgtccgaga taatgatgtt gctttcaaaa taatcaatac
     1321 tgagcgcaac aattttcccg gagtcaaact taagcattac tggcttaaaa gcagtttttt
     1381 catctcctga tgatattaca aaatcatctt ttctggttcc aaatctgtca taaacagcac
     1441 atttttgctc accacctata tataggtcgc catcctcgga gaactccata aaataaggcg
     1501 atacaagcct gttgctctgc ctcacaataa atggaaccat aagttgtgtt ttgaaatcag
     1561 gaccaacctt gcatatcttg aatttgtttt cagacaaccc ttcttccata aatcctgctg
     1621 ttatagccat gtaaaaagtg tctgatgatg tcttgtagac agaatttaca taaataccct
     1681 caagttggac catttttttg tctgtaagtt caaactgctc atcccatttt tccacaaaca
     1741 gcgcatcata acttacaaat attgcgtaaa aaccaccact tttcatggat aacagagctg
     1801 tcggaaactg gatgctactc attacttctt cttcaagtgc cttttctgga tcaactgtaa
     1861 aatctttcct accaagcaat ttgcctgttg gatcaaaaag aagcgcagac catttctttg
     1921 tttctttcaa accttctgtt atattgattg caatattacc attcggcagg actgtaaaat
     1981 tgaagaattc atcaatatcc tcgccatcac tatcaacaag acttattggt tcggcgtcct
     2041 cttgatcgac tttccagata gaattgtatt gatcaaggta atagaaattt ccctttgagt
     2101 cagtctatat accaacatca aggtaaattg gatcattaaa tttgtatttt tttataagct
     2161 tcagtgtttc gtcatagatt gccaagcaat cttgcgttgc aaaagcaaaa ttgttgctat
     2221 catttgcagc aaaagcaatt tttggggttc ttccatcaaa actgttggaa ttgttgtaga
     2281 ggaaactgta ccaacttcca ccaacatcag caacgagcgt cggtgggaag cctatacatt
     2341 ttgaaaaggc accttctgct gtccagagct ctacacctct cgaagtgaga agatacaaac
     2401 cttttttggt gggtgacaaa gacatgacat ccttgacttc tccatcaagg gctccatagg
     2461 aaaggagaac tggaggccca taatcagtct cttttggtat tctaagttct ttggtttcag
     2521 gttgggcgaa accatttgaa aatacttgtt ccacaggtgt ttctggcaat ttcacattat
     2581 ttggaaacag cacaaggaat gatccaggtt tattgtcctg ctcgattttg aatgagacct
     2641 gcgaaaggaa actggctgga acgcaaagac cagaatcaag catcattatt ggctgctgtg
     2701 actgcacatc cttttgtgcg ccacccattt caatcttagc attgcccgtt tcaagtgttc
     2761 cactcatagt gaaggaacta ttgccaattg accacctatc tccatccgtt gtcacatcaa
     2821 ggccaagcgt tttgccaact acagtaaaat tgacataagt ttcgttattt ctctggaaga
     2881 atggctgtgt tttcaggtcc ttggtgtagg tctggttttg gatgaaataa tttttgccaa
     2941 tggtccccgc gacattcttt tgtgtggcaa atgtgcaatt tatttttttc gatgaagaaa
     3001 acccttctat ggttgttaca ccaagttcca ggccttgacc agaaggctta acgtatactt
     3061 taaaatcgcc cttgtcggat accctggcaa gagagccctg acaattgatc aaagcacttt
     3121 tttgtgcttt tccggtcaca tagaagggtt tgtccggcaa gatcatgaaa tcggatatgt
     3181 tgagatttga aatggcttcc agtgagcctt cccttccttt cggaagaacc tggagtctat
     3241 aagttgaatc attgtttttt tcatttactt ccccttgcat ggtgatattg gcgccaagct
     3301 caaggattcc tggtgaatca ggtgtttcta tctcaaattc aagatttgtc ttgccaccct
     3361 tttctatctg tggaacagtc tttgagaaac caaagggctc caaaaccaat ctggcttctt
     3421 ttgatgccac attacctgaa tttacaacac tcacacttac ttttaccctt gaggatgtaa
     3481 gagcaactgg tggatcgcac gaaattgagc cactatatag cgaaacgtca ggttgcggcc
     3541 tgtggctgta ggttaaaagg ttatatagga atctgaaatg ttcagggtta gtattctcat
     3601 ataaggtcga gaaataaacc gaattgcctc cacttgtggc aattatgttc ttcccgtccc
     3661 tgctttttaa aacaattttt gccccgtcaa gacactcgtc ccatgattgg gcaggaattg
     3721 tgtttgacat aatggctctt gctaacatct ggccatcaat gccctggaag aaatcatttg
     3781 caggatcaac tggtacatca aacgtaaatg catcttccga caatccatac tcaagcgatt
     3841 gcttgaaacc aaaaaccttt gcataggctg tgtaattact gccgccagat tctgatccaa
     3901 ggcacgcgta gcctattgcc tggccgccat tctccacaaa tgaaagcagc ccttgcgcaa
     3961 atttgctgtc atacggattt gcaaaattgg gcgaggccat gatgactgtg tccgcaccaa
     4021 gctctgcaaa attctggtat ccaaaaccaa aaagcagcga catatagctg tcgtcgccgt
     4081 atgtcacaat ttcagttggg attttcttga aatcctttac cgagctttcg tatgaatcaa
     4141 tcccaaagac cttttttgcg gtcgatgcgt tgaaaaggta tgatatgaga caaagggctt
     4201 tggctgtttc acttttggtc aatacaagtt gtttctcaaa aacattgtct ttgggaaaat
     4261 catccttaaa atcggatttt acctcgaagt tgactgtatg ttcaccttct tcaagtccct
     4321 caaccatgaa attgagctgc tttaatgaat taatctccaa acttccaatt ttttttgttt
     4381 ttcttgtaac accatcaatt tttagctgag caacaatatt ttgcagattt ttataccctt
     4441 catttattac ctggaaagag aaattttgcc tttttgatgg tttgtcggtg tagaaaagcc
     4501 tgtttgaaac agcttttaga tcatattttg cccttgtaat ataatatatc cagatctccg
     4561 agctgtttgg caattcaatt ctggcttgcc acaaactttt tgtgttatgt gaaaatggca
     4621 ctttaaaggt cttgtttgtt gggtttgtaa gcttttgggc aggctgtgac ttggcagttt
     4681 caaggtcgat ttttcttgat tcaatccatt caaaatcagt tttcacaagt acctgattgg
     4741 gttcaaccac tgacatgatc gggatactca gtccatcgct agtcattgga caatcaagga
     4801 cctctatgtc tggcattcta tgaggcgcac caccactatt ttgtgggagt tttattgttg
     4861 gatcaccaag gaaagtgaaa cagaacaacg attttgatgt cgtgaagaac ccctcttttg
     4921 caacgccaac atattttttc aatgcctggc tgtagagctc tccaagggtt gttgcccaat
     4981 tgttgtaggc attgactacc tcagtggtaa gtctgtccat ctcatcaaaa ggtaatgctt
     5041 ttgcaacacc accctcaata gtccagttta ttcccgcata attgagcctg gatcctccaa
     5101 aatatgcgac agggccaccc tgagagaaga ggcaggcttg tgcaaaagag atgtccttgt
     5161 actctggcgt atccttaaat tctaccccga cgacagagtt gtcaaacatt ccattggtgc
     5221 atgcgacact caccagaacc ggcaatttat ctctcttcgt aagccccatt atatcagtat
     5281 ttgttatttt cccaggctca gtaatgattg cattgccagc accatggctc acgcaataaa
     5341 ccaaaccatt atcttcagta aaggccttaa gaagggattc cgaatcaaaa ctgtccctgg
     5401 ttttatattt cttggagatg ccaaacccat ccaggtatcc attatcaaca agtgactggc
     5461 attccagctc gccaaggaag ccgccactaa aaggatcacc cccggaaagg acaatttttt
     5521 tgaaccattc cggttttatg gattttctgt atctaacaat cttgtcgacg acatcatttg
     5581 cttctttgag gttcctgact ggcttgagtc gtaggaatgg gatatcatcc agaagaaggc
     5641 ccgctgtaaa atccggggca ttttttcgga accagccgca gaccagcggg ttgaacgact
     5701 ggacggcaga ttcacctttg tagtctt
//
LOCUS       DAOSRI010000092         5726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12802_length_5726_cov_3.872686, whole genome shotgun sequence.
ACCESSION   DAOSRI010000092 DAOSRI010000000
VERSION     DAOSRI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_5726_cov_3.872686"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1648)
                     /gene="hcp"
                     /locus_tag="PK717_03760"
                     /gene_synonym="priS"
     CDS             complement(5..1648)
                     /gene="hcp"
                     /locus_tag="PK717_03760"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOR46811.1"
                     /translation="MFCYQCEQTFGGKGCTVQGVCGKDDTTATLQDLLIYATKGIAQY
                     AKRARELGATDPEINRFTAEALFTTITNVNFDPNRLVELIYKAGRMKEKAKDLYEVAC
                     LKSSRVPDKLSGPATWTAAKSFEGLVEQGREFSPAKMIGRIGEQKAGLLQFMTHGLKG
                     ASAYADHAAILGVADDKFYEKLHKILDLISKEPTELGQLMDGLMDAGQANINAMEMLY
                     RANTNTYGHPVPTRVRMTAAKGKAILVSGHDLLDLEELLRQTEGKDINIYTHGEMLPA
                     HAYPKLRAYNHLVGNWGGAWQEQIHEFPNFPGAILMTTNCIQRPKQEYAQRIFTSGLV
                     AWPGVTHIRNRNFTPVIDAALQAPGFQSTEFPHEINVGFGFQTVVGIADKIVDMVKSG
                     KIRHFFLIGGCDGAKPGRSYYTDFAKMVPKDCVIMTLACGKYRFNKEDFGDIDGVPRL
                     LDVGQCNDAYSAVQIALALSQAFGVDVNELPLSLVLSWYEQKAVAVLLSLLALDIKNI
                     RIGPSLPASLTPDVIEMLHKEYNIQPITTPEQVLKAILG"
     gene            complement(1671..2399)
                     /locus_tag="PK717_03765"
     CDS             complement(1671..2399)
                     /locus_tag="PK717_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46812.1"
                     /translation="MGVRKIVKIDEEKCNGCGECVTSCVEGAIKIIDGKARLVSDSYC
                     DGLGACLGHCPQDAITIEERDAVDFDEKAATQHMSQSSKPQPQACGCPSSQARTVHRE
                     KQEGGAIPSAEQQSCLATWPVQLKLVSPHAPFLKNSHLLLAADCVPFAYANFHSKMLQ
                     GRPVVIACPKLDDISDYVQKLSDIIKLNGVKSLTIVHMEVPCCSGLVRIAQAAAAMSG
                     TNVPIKEITVMINGDAFDNFGSQI"
     gene            complement(2399..2824)
                     /locus_tag="PK717_03770"
     CDS             complement(2399..2824)
                     /locus_tag="PK717_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOR46813.1"
                     /translation="MASFVKLSEACTLAIHTMAFLACDDEGKLSARELSVQLKASFDH
                     LSKVLQRLTKADLVRPVRGPQGGFVLARPAGEINLLEVVESIEGRMEPSSCPLSKPFC
                     PTGGCTLGCVMKDVEAKLSDHYSKTSLEQLCQAIKKENR"
     gene            2976..5030
                     /locus_tag="PK717_03775"
     CDS             2976..5030
                     /locus_tag="PK717_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 and DUF1524 domain-containing protein"
                     /protein_id="HOR46814.1"
                     /translation="MEAKKVNLTKYMQNVHFIIPIYQRKYSWGIEECEQLWDDVLRVG
                     KDENCESHFFGAMVCVNNTKHHYETNKLLMIDGQQRLTTIFLLLLAISKTMDPDEAER
                     IHNQYLINSFKKDELRKKIILTAGDQEELEKLLQNKPIDEFAGSRIFENYYFFQEKLK
                     TESPQKIFCGVEKLEIVDVSLDPIDSPQLIFESLNSTGMSLTQSDLIRNLLLMEEGYE
                     KQSDLYHTHWRPIEERFSRADKEDRFDRFIRDFMTIQSDAGDISNLGEVYKNYKKYYL
                     KSRLTNEEALAELEQFSRHYEIIYKAKSSDKEIQSMLAELKILRFEVSHPFLLRIFDD
                     FEKNNITREQVLEIIRTIISYVVRRQILNLATNSQNKIFATLYSKLDKNYLLESFYAM
                     LLGQSDFDNFPTDDSFKSQLVMRNLYDMKNTPKYLLVKLENHTHKKEIINPEEYTIEH
                     ILPQNRKLSLEWKEMLGPDWEAVQNKYVNTLGNLTLTGYNSELSDRPFKEKQDTAYNT
                     SPLLLNKYLSELPKWGEEEIKARSTLLAEKAVSIWRYPAITSKVSEFIESRKEKQTRR
                     KFDLDDFNNRMDAKGQELLEEFRSGIFKLSPDINEIVNEICISYKVARNFLMINPRKT
                     SLLCELYGIEFEELDNPDIFCQKSPQNWWSNAHFRIKSKDDIPKAIRAVGQALAKQVN
                     TD"
     gene            5624..5726
                     /locus_tag="PK717_03780"
                     /pseudo
     CDS             5624..5726
                     /locus_tag="PK717_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase subunit GuaA"
BASE COUNT         1615 a         1254 c         1323 g         1534 t
ORIGIN      
        1 ttaattatcc aaggatcgcc ttcaggacct gctctggcgt tgttatgggc tggatattgt
       61 actctttgtg gagcatctct atgacatccg gggtgagtga tgccggaaga gacgggccga
      121 tcctgatgtt tttgatgtcc agtgccaaaa gtgagagcag gacagcaaca gccttttgct
      181 cgtaccagga aaggacgagc gagagcggca gttcgttgac gtcaacgcca aacgcctgcg
      241 agagggcgag ggcaatctgc accgccgagt aggcatcgtt gcactggcca acatccagca
      301 gtctgggcac tccgtcaatg tcgccaaaat cctctttgtt gaaacggtat tttccgcagg
      361 caagcgtcat tatcacgcaa tctttcggca ccatctttgc gaagtcggta tagtagctcc
      421 ttccgggttt tgcgccatca catccgccaa tcaggaagaa gtgccttatt tttccgcttt
      481 tgaccatgtc cacaatcttg tcggcaatcc cgaccactgt ctgaaaacca aaaccaacgt
      541 ttatttcgtg tggaaactcg gtggactgga acccaggggc ctgcagtgca gcgtcaatga
      601 ccggcgtgaa gttgcggttt cttatgtgcg tgacccctgg ccaggccaca aggccggagg
      661 tgaatatcct ctgggcatat tcctgttttg ggcgctggat acagtttgtt gtcatgagaa
      721 ttgcacccgg gaaatttggg aactcgtgga tctgttcctg ccatgcacca ccccagttgc
      781 caacaagatg gttataggcc ctgagttttg ggtacgcatg ggctggcagc atctcgccat
      841 gggtgtatat atttatgtcc ttgccctcgg tttgcctaag cagttcctca agatccagca
      901 ggtcatggcc cgaaacaaga attgcctttc cctttgctgc tgtcattctt actctggtcg
      961 ggacaggatg tccataggtg ttggtgttgg ccctgtaaag catctccatt gcgttgatgt
     1021 ttgcctgtcc ggcatccatc aggccatcca tgagctggcc aagttcggtt ggctcctttg
     1081 aaattaaatc aaggattttg tgcaattttt cataaaactt gtcatcggcg acaccaagga
     1141 ttgcggcgtg gtcggcatat gccgatgcac ccttgaggcc gtgggtcatg aactggagca
     1201 agccggcttt ctgttcgcca atgcgcccga tcatctttgc aggcgaaaat tctctcccct
     1261 gctctaccaa cccttcaaag gacttggccg ctgtccatgt tgctggcccg gagagtttgt
     1321 ccggcaccct gctggatttc aggcaggcaa cctcatacag gtcttttgcc ttttccttca
     1381 tgcgtcctgc cttatagatc agctctacaa gcctgtttgg gtcaaaattc acgtttgtga
     1441 tggttgtgaa aagcgcctcg gcagtaaacc tgttgatctc cgggtcagta gctccaagct
     1501 cccttgccct ttttgcatac tgggctatgc cctttgttgc ataaattaga aggtcctgca
     1561 gggtggccgt cgtgtcgtcc ttgccacaaa cgccctgcac tgtgcatccc ttgcccccaa
     1621 aagtctgttc acactggtaa caaaacatgg cttttctcct tttctataat ttatatctgg
     1681 cttccaaagt tgtcaaaagc gtctccgttt atcatcactg taatttcttt gatcgggaca
     1741 ttggtcccgg acatcgcggc tgctgcctgc gcaattctga caagcccgga gcaacagggc
     1801 acttccatgt gcacaatggt cagactcttc acaccgttca attttatgat gtctgacagt
     1861 ttttgtacat agtctgaaat atcgtccagt tttggacagg caatcacaac aggcctgccc
     1921 tggagcatct tgctgtggaa gtttgcatag gcaaacggaa cgcagtctgc ggccagaaga
     1981 aggtgtgagt tcttcaggaa cggcgcatgt ggcgagacca gctttagctg gaccggccag
     2041 gttgcaaggc aggactgttg ttctgctgat ggtattgcac caccttcctg cttttccctg
     2101 tggaccgtcc ttgcctggga ggatggacag ccacaagctt gtggttgcgg cttgcttgat
     2161 tgcgacatgt gctgtgtggc cgccttctca tcaaaatcca ctgcatccct ctcctcgatt
     2221 gttatcgcgt cctgggggca atggccaagg caggcgccaa gaccgtcaca gtatgaatct
     2281 gacacaagcc tggcttttcc atcaataatc tttattgcac cttcgacaca agaagtgacg
     2341 cattcaccgc acccattgca tttctcctcg tcaattttga caatcttgcg aacacccatt
     2401 atctgttctc ctttttgatc gcctggcaga gctgctcaag cgaagttttg ctgtaatggt
     2461 cagaaagttt tgcctcaacg tccttcatta cgcagccaag cgtgcacccg ccggtggggc
     2521 aaaacggctt ggaaagaggg cacgaggagg gttccatccg cccctctatc gactccacca
     2581 cctcgagcag gtttatttct ccggctggcc tcgccaggac aaacccgccc tgcgggccgc
     2641 ggactggcct gaccaggtca gcttttgtga gcctctggag aacctttgaa aggtggtcaa
     2701 acgatgcctt gagctgaacc gagagctctc ttgccgagag ttttccctca tcgtcgcagg
     2761 ccagaaaagc catcgtgtgg atagccaacg tgcaggcttc ggaaagtttt acaaagctgg
     2821 ccatttttgc ccccttgtgt ttcccttttc tgtttttgca tctctcttat taagcatact
     2881 ggtacttata taccagttat gaaatggatg tcaagaggtt gagaatttat ggaaaagaaa
     2941 atagaattta tttagcaaat ttaaaggtga gggttatgga agctaaaaaa gttaatttga
     3001 caaaatatat gcaaaatgtg catttcatca ttccaatata tcaaagaaaa tatagttggg
     3061 gtatcgaaga atgcgagcag ttatgggatg atgtattaag ggtgggcaag gatgaaaatt
     3121 gtgaaagcca tttttttggt gcgatggttt gtgtcaataa taccaagcac cattatgaaa
     3181 caaacaaact tttgatgatt gatggccagc aaagacttac aactattttc cttcttttgc
     3241 ttgccatttc aaaaacaatg gacccagatg aagcagaaag aatccacaat caatatctaa
     3301 tcaattcttt taaaaaagac gaattgagaa aaaagataat tcttacagcc ggagaccagg
     3361 aagagcttga aaagcttttg caaaacaaac ccatagatga atttgcaggt tccaggatat
     3421 ttgagaacta ctattttttc caggaaaaac taaaaacgga atctccacaa aaaatttttt
     3481 gtggagtaga aaagctggaa attgtagacg tttctttgga tccaatagat tctccccagc
     3541 ttatttttga aagcctcaac tctacaggca tgagccttac gcaatcagat ctaataagaa
     3601 atttgctttt aatggaggag ggctacgaaa aacagtctga tttgtatcac acccactgga
     3661 gacctataga agaaaggttc tcaagggccg acaaggagga caggtttgac aggttcatca
     3721 gggacttcat gacaatccag agtgatgcag gtgacatatc aaatcttggc gaagtgtata
     3781 aaaattacaa gaaatactat ctgaaaagca gactcacaaa cgaagaggca cttgcagagc
     3841 ttgagcaatt cagtagacac tatgaaatca tctacaaggc aaagtcttca gacaaggaaa
     3901 ttcagagcat gcttgcagaa ttgaaaattc ttcgttttga agtctcgcac ccattcttgc
     3961 tcaggatttt tgatgatttt gaaaaaaata acatcacaag ggaacaggtt cttgaaataa
     4021 tcaggacaat cataagctat gtggtcagaa gacagatact caatttggca actaacagtc
     4081 aaaacaaaat atttgcaacg ctttatagca aactggacaa gaattatctg ctggaatctt
     4141 tttatgccat gttgctaggg caatcggatt ttgacaattt tccaacagat gacagtttca
     4201 agagccagct tgtaatgaga aatctctacg atatgaaaaa cacaccaaaa tatttgcttg
     4261 tcaaacttga gaatcataca cacaaaaaag agatcataaa tccagaagag tacactattg
     4321 aacatatttt gccacaaaac aggaaactat ctctggaatg gaaagagatg cttgggcctg
     4381 actgggaggc ggtccagaac aaatatgtaa acacactggg taatttgacg ctaacaggtt
     4441 acaactccga attgtcagac aggccattca aagaaaaaca ggatactgcg tacaacactt
     4501 ccccgcttct attaaacaag tacctttcag agttgccaaa atggggtgag gaagaaatta
     4561 aagccagatc gacccttctt gccgaaaaag ccgtatcgat atggcgatac cccgcaatta
     4621 caagtaaggt ttccgaattt atcgagagca gaaaagagaa acagaccaga agaaaatttg
     4681 atctggatga ttttaataat aggatggatg caaaaggaca agaactgctt gaagaattta
     4741 ggagtggaat cttcaaacta tcaccagata taaatgaaat tgtaaacgaa atttgcattt
     4801 cttataaggt tgcaagaaat tttctcatga tcaatccaag aaaaacttca ctcctgtgtg
     4861 aattgtacgg aattgaattc gaagagctgg acaatccgga tattttctgc cagaaatcgc
     4921 cacagaactg gtggtctaat gcccacttca ggattaaatc caaagacgac attccaaagg
     4981 caattcgtgc agttggacaa gctttggcaa aacaggtaaa cacggattaa gttgttgaga
     5041 ttcaaatatt ggatcttctt gggaattatt taggaaaatc catgagtgta gggaagagta
     5101 tggcaaatgt ggacacaatg tccacagcaa gggggcaaag agtatttgtt gggataaaga
     5161 acatttctta tgcgtgttag gaagtattcc atgtggaccc catgtgtgtg ggaaaaagcg
     5221 ctgataaccc gcttttatat tgtctctctt atacatgtaa taataaaatc ttctatcgta
     5281 ataaaagtga taaagaaatt gtgcttatta catgtactaa tttgtaattc ctggaaaaat
     5341 ttgacgggtg aatttgatat aggcagggtt tcctttgtat ataataatta tcctttatgt
     5401 acagaggtgc aaacatacta ggtagctcat caattgagaa agtaagtcca tcaaaggcaa
     5461 gggagaacct tgtagagata actgacaagg tgaggtttgg gaagaagagg cctgtttttc
     5521 tcctcaaggg aggcaaggac attaccgcaa ttgtgcccat tgaagacaag attgatattg
     5581 aatggctgaa caagccctta aaaatggaat tacctattca ttgcatgagt tgctggacag
     5641 gattgcaacc cgcatcacag gagaggtgaa acagatcggc agggtggttt ttgacatcac
     5701 aaacaagcca ccagccacga tcgagt
//
LOCUS       DAOSRI010000093         5710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_12855_length_5710_cov_4.867020, whole genome shotgun sequence.
ACCESSION   DAOSRI010000093 DAOSRI010000000
VERSION     DAOSRI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_5710_cov_4.867020"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2494
                     /locus_tag="PK717_03785"
     CDS             <1..2494
                     /locus_tag="PK717_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46815.1"
                     /translation="LSNKKVNWTMPAAERSYYDVRVFASPDPPFSLGELTSDKKSLAN
                     GAFLDQLSQGYFPENGFARENVKFNACQPYTVVPVIVAQDNFLDFMFRPVGTVFEEVG
                     GEYVQKPSSESACAIKSIFHDVIFDQTSSSMGFDGVMDMTIKTKLRYKNEFRFDYSVD
                     PPEIAGLSALPLATSNNGVSSMFEQSGKNHLVQGFMSLGKLYRWMPQTDPAYPNQKLL
                     IKPAVRDRVLADGTYWELSKFRVHFPDATLVDGKVEVTEKFVDVSPIKFDEISQGRFA
                     YFTFTNNPLLEGADSTFFVEKIDRPFRVEAVYEPGRLGARGKQNTAESTRLKLFWSAT
                     PQMKDLAKRKTQEYGENWWKKAFPTYYKIVLKGPDGQKTFDKINPMQESMIADGLKPG
                     AEYSWELQTWVSDAEGSPQLVETWKSDFVKTKPLPLLVGHCGQPSIIKVKTAVDVDGD
                     EVWVISAPMAYELGLTKFQPLYFMAKPTSFGQFEKLLKSKTYYTVVEKDDQNYLRAYI
                     KVSAFSDSYEKDDQGRNIIHLEFQPVEVRVEGEAVDEFMQPIGKSLGGHAGTWWPARD
                     GPSTSSLRVVEAPLLVKTNKLYRFRRWTWVGPIYALRTPIEDAEKGIAVLFANVDGCQ
                     ANPDKYVKPKIYAVYDTVPVDCFTVTSDFAINWKPVAYSGRIPIKPEGDCPGNSSAYM
                     AGTQVSIGPAPKEIVIEELKYKFVGWIIGENSIPGKEVLTINVDKSYNVLANYQPAND
                     RYELFFFTDADGRRIDLRLSVNPPPDKDGLYKAGTVVTVGPAPLELDDGKYSFAGWAL
                     DGNIVGTDFRTNSIQVTMDKAHRLICLYKLGG"
     gene            2520..3161
                     /locus_tag="PK717_03790"
     CDS             2520..3161
                     /locus_tag="PK717_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46816.1"
                     /translation="MKQNGVKLSTAIILAIVVGIASYFATAFFIGAWPFEKAKQPTTK
                     DLLKESGSQDGSLEGLFPDGKGTYGTSDGSQKTFDGFSIAFPDKWLYMRMQDSGVVTG
                     LDSLDPMASVQVLKLSGKDVPEGDPEKALDEILKTTDDSKIEIISREKVKFLDKDCSL
                     FLGKTKRMDKEIGMITLVVPSSKDAYVVMGTFNVDDGKTKEIVEDVIATFRLE"
     gene            3168..4127
                     /locus_tag="PK717_03795"
     CDS             3168..4127
                     /locus_tag="PK717_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOR46817.1"
                     /translation="MLALTRILQLLDMLLQAGSRKLTSAEISNKLEISRRQIIRDIDK
                     LRDEGFNIRSDMNGYWLETAPDLGSFGLSKFDKFVITTGLGRIIDSKDPCLSRIAQEI
                     IEKITNENPQTGREKPVDEFKVVYLNEIKKIQKIRVAIDSMVVINFKYERPNKLDEKL
                     RKVKPYELFFKRHAYYMIAMPEDSDTLIMYRVNRMKDITLTRERFVRRPIDLNSHLSN
                     AFEVMITGPVEDVEILFKKSVAPFVSELVWHSTQKITKNDDGSIIFKARVAINHEILR
                     WVMGYGNECVVAKPEQLKQMVIEQTKLMLEDYLDQKSPNVINA"
     gene            4261..>5710
                     /locus_tag="PK717_03800"
     CDS             4261..>5710
                     /locus_tag="PK717_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46818.1"
                     /translation="MKRTLCALLVFVLAAPVVFSATSDSITIPSFAKYKDNPVLLARK
                     AKPGESKYFDSSAVTDPCVIYWEGRYHMWYTGWINWMGGSGRQGRIGYAISPDGKKWT
                     RVDGTAGGIEAPGAVLGTGASNEYDGFSVQSPWVINDNGKLRMYYVAVDTKGKMTLGL
                     AESNTGNYWNKKGQILMPTNELAFDGFQVGKCSVVRVNNIFKMWYEGQSKIDSRWRIG
                     YATSYDGSTWTKNIGEKTGGAVLDLGDKITDFDNFTVRAPSVVFDGVYYYMWYDTSMR
                     DEKTSQIGIATSVNGISFEKKGVSLDVGKIGDFDAQRVASPGVLKNGLVFYLYYCNYS
                     DTNSGIGLATSGDLPANIGSGVVKLDCKIGDGFDFSLGNKTIDPTLADLVVDCGFGAP
                     RMCGRYIRNAVDFADAECVPAEGYPAVSSVKENCQNIEVGATYAFKTINEQNYAKIKI
                     EGIWQDPVDPNHYEITIKWMYQSNGSPCFIGGS"
BASE COUNT         1647 a         1232 c         1421 g         1410 t
ORIGIN      
        1 cctttcaaac aaaaaggtca attggacaat gccggctgct gagcgcagtt actatgatgt
       61 cagggtgttt gcgtcgccag atccaccctt ctcactagga gaactgacca gcgacaaaaa
      121 atctcttgcc aatggggcct ttcttgacca gctttcccag ggctatttcc cggagaatgg
      181 ttttgcaagg gagaacgtaa aattcaatgc ttgccagccg tataccgttg tacctgttat
      241 tgttgcgcaa gacaatttcc ttgatttcat gttcaggccc gtcgggacag ttttcgaaga
      301 ggttggtggg gaatatgtcc aaaagccctc ttccgagtca gcatgcgcaa taaaatccat
      361 ttttcacgat gttatttttg atcaaacaag ctcttcaatg ggttttgatg gtgtcatgga
      421 catgacgatc aaaaccaagt tgagatataa aaatgagttc aggtttgact attcggttga
      481 tccaccggaa atagccggtc tttcagcgtt gccgcttgca acatccaaca atggtgtttc
      541 aagcatgttt gaacagtctg gcaaaaacca tcttgttcag ggtttcatgt cactgggaaa
      601 attgtacagg tggatgccgc aaactgaccc ggcgtatcca aaccagaaac tgctcataaa
      661 gcccgccgtc agagacaggg tacttgcaga tgggacttac tgggaacttt caaaattcag
      721 ggtccatttt ccagatgcca cgcttgtgga tgggaaggtg gaggtcaccg aaaagtttgt
      781 tgatgtctcc cccatcaagt ttgatgagat atcacagggg agatttgcat acttcacttt
      841 cacaaataat ccactgctgg aaggcgccga ttcgaccttc ttcgtggaga aaatagacag
      901 gcctttcagg gttgaggcag tgtatgaacc gggcagactt ggtgccagag gaaagcagaa
      961 cactgccgaa tcaacaaggc tcaaactttt ctggtcggcg acgccacaaa tgaaggacct
     1021 ggcaaagaga aaaacccagg aatatgggga aaactggtgg aaaaaagctt ttccaactta
     1081 ctacaagatc gttctcaagg gtccggacgg ccagaaaaca tttgacaaga taaatcccat
     1141 gcaggagtca atgattgccg atgggttgaa accaggcgcc gaatactcat gggaactaca
     1201 gacgtgggta tctgatgccg agggttcacc ccagcttgtc gagacctgga aatccgattt
     1261 tgtcaaaaca aagcctttgc cgctccttgt cggccactgt ggccagcctt caatcatcaa
     1321 ggtaaaaaca gccgttgatg ttgatggtga tgaagtttgg gtaatatcgg ccccaatggc
     1381 gtatgagctg ggcctgacaa aattccagcc gctctatttt atggccaagc caacatcttt
     1441 tgggcaattc gagaaactcc tgaaatcaaa aacatactat acggtcgttg aaaaagatga
     1501 tcaaaactat ctcagggcct acataaaagt atcggccttc tccgactcgt atgaaaagga
     1561 cgatcaggga agaaatatca tccaccttga attccagcct gttgaagtca gggtggaagg
     1621 cgaggctgtt gatgaattca tgcaacccat tggcaaatcc ctcggcggac atgccggaac
     1681 gtggtggcct gcgagggacg gcccgtcaac ctcgtcgttg agggttgttg aggcccccct
     1741 gcttgtcaaa accaacaaac tctatcgctt cagaagatgg acctgggttg gtccgattta
     1801 tgcactgagg accccaatcg aggacgctga aaagggtatc gccgttttgt ttgccaatgt
     1861 tgatggttgc caggcgaacc ctgacaaata tgtaaagcca aagatatatg ccgtttatga
     1921 tactgtgcca gtggattgtt ttacggtcac ctcagatttc gcaatcaact ggaaacctgt
     1981 tgcttacagt ggaagaatac ctataaaacc ggaaggtgat tgcccgggca actccagcgc
     2041 gtatatggcc ggaacacagg tatcaatcgg cccggcacca aaagagattg ttattgagga
     2101 acttaaatac aaatttgttg gttggataat tggtgagaat tcgattcctg gaaaggaagt
     2161 cttaaccata aacgttgaca agtcatacaa cgtcctggcc aactaccagc cggccaatga
     2221 taggtatgag ctgttcttct ttactgacgc tgatggcagg cgcatagacc tcagactgag
     2281 cgtaaatcct ccaccagaca aagacggtct ctataaggcc ggaactgtcg taacggtcgg
     2341 cccagcacca ctagagctcg atgatggcaa atactcattt gcaggttggg cgctggatgg
     2401 caatattgtt gggactgatt tcaggacaaa ttcaatccag gtgaccatgg acaaagccca
     2461 caggctcatc tgtctctaca agctcggcgg gtagcttgta acagtgaaag gaatgattga
     2521 tgaaacaaaa cggtgttaaa ctctctactg caataattct tgccattgta gttggaattg
     2581 cctcatattt tgcaacagct tttttcattg gtgcgtggcc ttttgagaag gcaaagcagc
     2641 caactacaaa agaccttcta aaggaatctg ggtcacagga tggctcactt gaaggcctgt
     2701 tccctgatgg aaaaggcact tatggcactt cagatggttc gcaaaagaca tttgatggtt
     2761 tttccatagc ctttccagat aaatggctct atatgagaat gcaagattct ggcgttgtta
     2821 ctggcctgga ttcgcttgat ccgatggcct cggttcaggt gctaaagctt tcggggaagg
     2881 acgttcccga aggtgacccg gaaaaggcac tggatgaaat cctcaaaaca accgatgata
     2941 gcaaaatcga aatcataagc agagaaaagg taaaatttct ggacaaggac tgttcacttt
     3001 ttcttggcaa gaccaagagg atggacaagg agatcgggat gataacgctt gttgtgccaa
     3061 gcagcaagga cgcttatgtc gtaatgggca cttttaatgt tgatgatggc aaaaccaaag
     3121 aaattgttga agatgttatt gcgacattca ggctagaata gaaataaatg ctggcgctta
     3181 caagaattct gcagctcctt gacatgttgt tacaggcagg tagcagaaag ctcacaagtg
     3241 ctgaaatatc aaacaagctt gagatttcaa gacgccagat cataagggac atagacaaac
     3301 tcagagacga aggtttcaac atccgctctg acatgaacgg ctactggctt gaaactgcgc
     3361 ctgatttagg cagtttcggg ttgagcaagt ttgacaaatt tgtgattaca acagggcttg
     3421 ggaggataat cgattcaaaa gacccgtgtc tttcaaggat agcccaggaa atcattgaaa
     3481 agatcacaaa cgagaatccc cagactggta gagaaaaacc ggtcgatgaa ttcaaggttg
     3541 tctatctcaa cgagataaaa aaaatccaga aaataagagt tgccatagac tcaatggttg
     3601 tgatcaattt caagtacgag cgtcccaaca agctcgatga aaagctcagg aaagtcaaac
     3661 catacgagct ttttttcaag agacatgcct actatatgat tgcaatgcca gaagacagcg
     3721 acactcttat catgtacaga gtcaacagga tgaaagatat aacactcacc agagagaggt
     3781 tcgtcagaag gcccatagat ttgaacagcc acctttcaaa cgcttttgaa gtgatgatta
     3841 ctgggccggt tgaagacgtc gaaatactgt tcaaaaaaag tgttgcgcct tttgtgtcgg
     3901 agcttgtatg gcacagcacg cagaaaataa ctaagaatga tgatgggagc attattttta
     3961 aggcaagagt tgcgatcaac cacgagattt tgcgctgggt gatgggatat ggtaacgagt
     4021 gcgttgtagc caagccagag caactcaagc agatggtcat agagcaaaca aaactaatgc
     4081 tggaggacta tcttgaccaa aagtcaccaa atgtcattaa tgcttgacat aaaaatatat
     4141 ttgtgctagt ttgcaatcag tattatactt taatgtatat ttcatgtgat aaagcgacat
     4201 caatttgggg atgaaccaaa atggtggtca gcgcgtacct ttttttgaaa ggagaaagcc
     4261 atgaaaagaa cgctttgtgc gcttcttgtt tttgtgcttg ctgccccggt cgttttttcg
     4321 gccacatcag acagcataac cataccgtct ttcgcaaaat ataaggacaa tccagtcctt
     4381 cttgccagga aggcaaagcc tggcgaatca aaatacttcg actcctccgc tgtgaccgac
     4441 ccatgcgtca tctactggga aggcagatac cacatgtggt atacaggctg gatcaactgg
     4501 atgggtggct ctggcagaca agggagaata ggctacgcaa tctcgcccga tggcaagaaa
     4561 tggaccaggg tggatggcac tgctggtgga attgaagctc cgggagcagt ccttggaact
     4621 ggcgccagca atgagtatga cgggttctct gtccagtccc catgggtcat caatgacaat
     4681 ggtaaactca gaatgtatta tgttgccgtt gacaccaaag gcaaaatgac ccttggcctt
     4741 gccgagagca ataccggaaa ttactggaac aagaagggcc agatactcat gcccacaaat
     4801 gagctggctt tcgatggttt ccaggtcgga aagtgctcgg ttgtaagagt caacaacatc
     4861 ttcaagatgt ggtatgaggg ccagtccaaa atcgattcaa ggtggaggat cggctatgcc
     4921 acgtcgtatg atggttcgac ctggaccaaa aacattggtg aaaaaactgg cggagcagtc
     4981 cttgaccttg gcgacaagat aactgatttt gacaacttca cggtcagagc gccaagcgtg
     5041 gtctttgacg gtgtctatta ctatatgtgg tatgacacct cgatgaggga cgagaaaacc
     5101 agccagattg gaatcgcgac ctcagtcaat ggaataagtt ttgaaaagaa gggtgtttcc
     5161 cttgatgtcg gcaagatagg tgattttgat gcacagagag ttgcctctcc tggtgtcctg
     5221 aagaatggcc ttgttttcta cctttattac tgcaattact ccgacaccaa ctctggcatt
     5281 ggccttgcga cttcaggtga tttgccggcc aacatcggat cgggtgttgt gaagcttgac
     5341 tgcaagatcg gtgacggttt tgacttctcg cttggcaaca aaacaattga tccgacactc
     5401 gctgatctcg ttgtggactg cggttttggc gcccccagaa tgtgtggcag atatataaga
     5461 aacgctgttg attttgccga tgcagagtgt gttcctgcag agggctatcc agccgtttca
     5521 agcgttaagg aaaactgcca gaatatcgaa gttggcgcga cctatgcttt caagaccatc
     5581 aacgagcaaa attatgccaa gatcaagata gagggcatct ggcaggaccc agttgacccg
     5641 aaccactacg aaataaccat caagtggatg taccagagca atggttcgcc ctgtttcatt
     5701 ggcgggtcaa
//
LOCUS       DAOSRI010000094         5653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13030_length_5653_cov_3.780636, whole genome shotgun sequence.
ACCESSION   DAOSRI010000094 DAOSRI010000000
VERSION     DAOSRI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_5653_cov_3.780636"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1191)
                     /locus_tag="PK717_03805"
     CDS             complement(25..1191)
                     /locus_tag="PK717_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR46819.1"
                     /translation="MNSVANQKQETKRVNIAPYIIVPLLIILLTFGGLFLYVYSKRDV
                     ARIVVDFPITERMIYLEIKNMERAGVKPQTNSTATEIQEFYSTARTKAITNLTDFFVV
                     LQKAKDTKQYDIPNEQIEQRIKDVISISQLNTLDEYLTEGEVSESEFRTQVRNQLVYE
                     KMTEPLRAEVGQPTTTELEEYFNTIKDQLVVPETVDYEVIVVPDEKTRNKVLEELTKG
                     KGNNFAEVAKKYSTDLASRENGGKMIAVPKESITEVEVSNALFPPTPTFPLKLEQGII
                     QPIVTQKSGEYIIRLNRRNPAQQATLYGTISLYNSETKKYEKVEIKPQVISMWQSSKG
                     DSAVSAYVKRLSDYYESRIYDRVKGNMPWAGLEKFFSSIFGNSLWNNIMGVEDK"
     gene            complement(1207..2988)
                     /locus_tag="PK717_03810"
     CDS             complement(1207..2988)
                     /locus_tag="PK717_03810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR46820.1"
                     /translation="MGKESKIRELRRAKLIPQVKETKIYKPMSKGWRVTIWTIIFVII
                     ALLGFGIWAYSGRKIEARVGSASITTDELEQKAYQLIQQQVSQDQMPQFGTEEFQKML
                     TSAKEQVIQQLISEKLFLQLGERENIKIEDKVYQDEAQKLIDQQKPKEGNAAEWIKQQ
                     GFENEVKMREFIVANNKSQLTVQIYMNKLFEKYVKEPEVTDQEALDFYNSNAELKLSH
                     ILLKYDSTKDAADVGTKKKTQIDEIRAQIMKDPKKFPEIAKQKSEDDATKPNGGDLGW
                     YKIQNGQLVTDQGNSLVPEFNDAAIKMKVGEVSEPVRTTYGWHLIYITDAKKNSEKYN
                     PTEAARIATIRWVGTNKNDPNTALTADELKKKETQANQVLKDLLAGKITFAKAAEQYS
                     EDDLSKFNSGELPSMLSTDSSGFFWAELQKAKEAQAGSYPYEKEVVEAAWGLKPGQVY
                     SKVLKAGKDIVIVKLITKRNYEKLPFESVKQEVIKQLKAQKKSEQQSKWLEEERNKLG
                     VSIGNPWKSFTVWWQSNIVAPFEDFGLWVGKLMGKGVGDGTTTTTTNQTQTLPSEAEL
                     QKILQDKGIQNPGSQIPTQQTPATNQP"
     gene            3297..>5653
                     /locus_tag="PK717_03815"
     CDS             3297..>5653
                     /locus_tag="PK717_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46821.1"
                     /translation="MRKIISLLSCFAIAFQIFVPAQNTTLASRVAFVDGFDLGAEAGD
                     GVIKLTWERVPGASGGYYVYRKDGENGTYGAPLTDFGVFGNVFYDGPIPNDNKYCYYI
                     APLDENYTEFGRSVEACSSPISQAPSFIEQCKTVLVFVVDSVNYLHNGVKRIMSSPFL
                     LVGRRTYLAFRYVTEPLGGKIEWDQVQRKVTVTYKSTVVEMWVGKSQAKINGKPVQID
                     PSDPKVVPFVKDGRTFVPLRFPVESFGEGDVKWFAQTKQAQLTFPADCNEKLKGKVIS
                     YNNKTGMSEILGFEGESYVVVFGQSGSGSYIPVVGDCIEISGERKRKGTHEYFFVNSI
                     SLIECQEGPFAGFGAVEKSDILSASATISFEGGEKIDVRIDKAHIRMLAGLKLGDCVF
                     AAGMVIKNPSGEKYINTIIFDMVPCKKGQIDIVCDGKWYSGTVRDFDCTNGILTVALD
                     DGQSKTVSIENSNFDCANLSVGYCTRFCAKEVDGKLVSSVFYAFPCGSSSCEGSLIRG
                     KLVKIDCASLSMSIAQGDKYVEVAIFPKDCEDISSSMPECVEACVVKSGKRFIALKVK
                     PYAECENECPGETVQVRIDRVDCAAKIIYCTKVATMEQMEINFTLPEFCPLEAGKCYE
                     ICIEEIKKPGQQPESIATHFRPIECKYRCSSQLVTGTIKSIDCSKQVVVISQNDNLGD
                     IIIRVTEQDCIRLKEDRCVKACITKDPTGLYMANWITEVGPEGCTQKIDCNGDDLWTI
                     RTTKIDCAGKKLEYEMGDRKGQLRVNDWSLCNSIKTGLCYSVCVK"
BASE COUNT         1417 a         1156 c         1292 g         1788 t
ORIGIN      
        1 cgattaaagc ccttatggtt ttgctcattt gtcttccacc cccattatat tgttccagag
       61 ggaattgcca aatattgatg agaagaactt ttcaagacca gcccatggca tgttgccctt
      121 aactctgtca taaatccttg actcataata gtctgagagt ctttttacat aggctgaaac
      181 agcagaatcg ccctttgagc tttgccacat gcttataacc tgtggcttga tttcaacctt
      241 ctcatatttc ttggtttcag aattgtaaag gcttatggtg ccataaagtg tggcttgttg
      301 tgcaggattt ctcctgttca atcttattat gtattcccca ctcttttggg tgacgattgg
      361 ctgtatgatt ccctgctcca gtttaagcgg gaaggtgggt gttggtggga agagtgcatt
      421 acttacttcg acttcagtta ttgattcttt tggaactgct atcattttcc cgccgttttc
      481 acgggaagcc aggtcagtcg agtacttttt tgcgacttca gcaaagttgt tgccttttcc
      541 ttttgtaagt tcctcaagaa ccttgttacg tgtcttctcg tcaggtacga ctattacctc
      601 ataatcaaca gtctccggaa ctacaagctg gtcctttatt gtgttgaagt attcctcaag
      661 ttccgtagtt gttggttggc ccacctcagc tcttagaggt tctgtcatct tttcgtagac
      721 gagctggttc cttacctgtg tcctgaattc ggattctgat acctcacctt cggtgaggta
      781 ttcatcaaga gtattcagtt gggatattga gattacatct ttgattcttt gctcgatttg
      841 ttcgttagga atgtcatact gctttgtatc ttttgctttt tgcagaacaa cgaagaagtc
      901 tgtgagattt gtaattgctt ttgtccttgc agtgctgtaa aactcctgga tttctgtcgc
      961 tgtggagttt gtttgtggct ttacgccagc ccgctccatg tttttgatct caaggtaaat
     1021 catgcgctct gtgattggaa aatcaactac aatccttgct acatcgcgtt ttgaatacac
     1081 gtacagaaaa agcccgccaa aagtcaaaag tattatgagt agcggaacaa ttatgtacgg
     1141 cgcaatgttt acgcgctttg tttcctgttt ttggtttgcc actgaattca ttcctccatt
     1201 tacctgttac ggttgattag tagctggagt ctgctgggtt gggatctggg aacctggatt
     1261 ctgtatcccc ttatcttgaa gaattttttg aagttccgct tcggatggca gggtttgagt
     1321 ctggttagtg gttgttgtgg tagttccatc accaacaccc tttcccatta gttttccaac
     1381 ccatagccca aagtcttcga atggggcaac aatgtttgac tgccaccata cagtaaagct
     1441 cttccatgga ttgccaattg aaacgccgag cttgtttctc tcttcctcaa gccactttga
     1501 ctgctgttcg gattttttct gggccttgag ctgttttatt acttcctgtt tgacagattc
     1561 gaatggaagt ttctcatagt ttctttttgt gatgagcttg actatgacga tgtctttgcc
     1621 tgcttttaaa accttcgagt atacctggcc tggtttaaga ccccatgcgg cttcaactac
     1681 ctctttttca tagggatagc ttcctgcttg tgcttctttt gctttctgga gctctgccca
     1741 gaagaagcca gaactatctg ttgaaagcat gcttggaagc tcaccggaat tgaattttga
     1801 gagatcgtct tcagagtatt gctctgcagc ttttgcaaaa gttatcttgc cagcaagcaa
     1861 atctttgaga acctggtttg cctgtgtctc tttcttttta agctcatctg ccgttagtgc
     1921 tgtattcgga tcattcttgt ttgtgccaac ccagcggata gttgctattc tagcagcctc
     1981 tgttggattg tatttttcag agttcttttt tgcatcggtg atatagatca agtgccagcc
     2041 gtaggttgta cgcactggtt cagaaacttc tccaaccttc atcttgattg cggcatcatt
     2101 aaattcggga acaagtgagt tgccctgatc ggtgacaagt tgaccattct ggattttata
     2161 ccagccaagg tctcctccat ttggttttgt tgcgtcatct tcggactttt gtttcgcgat
     2221 ctctgggaac ttctttgggt ccttcatgat ctgggcacgg atttcatcaa tctgtgtttt
     2281 cttttttgtg ccgacatcgg ccgcgtcctt tgtgctatcg tactttaaaa ggatgtgcga
     2341 caacttgagt tctgcgttgc tattgtagaa atcaagcgct tcctgatcgg taacttcagg
     2401 ttccttgacg tatttctcaa agagtttgtt catgtagatt tgcactgtca gctggctctt
     2461 attatttgct acgatgaatt ccctcatttt gacttcattt tcgaaacctt gttgcttaat
     2521 ccattctgca gcgttgccct cttttggttt ttgctgatcg atgagctttt gagcctcatc
     2581 ctgatatact ttatcttcga tcttgatgtt ttctctctca ccaagctgaa ggaaaagttt
     2641 ctcagagata agctgttgaa ttacctgctc ttttgcagaa gtgagcatct tctgaaactc
     2701 ttctgtacca aactgaggca tctggtcctg tgatacctgt tgctggatca gctggtatgc
     2761 cttctgctcc agttcatcag ttgttattga tgctgagcca accctggcct caatcttgcg
     2821 acccgaatat gcccaaattc cgaagcccaa aagggcaata atgacaaaga tgatcgtcca
     2881 aatggtgact ctccaccctt ttgacatcgg tttgtaaatc ttcgtctctt tgacttgcgg
     2941 gatcaatttc gccctgcgaa gttctcttat cttactttct ttacccattg tatttacctc
     3001 cgagcaatta acaaataacc attttatgcg gtgtcaactc acatgtaaag taagtgttcc
     3061 acttcaccta aacctgagcg ttacacatgg ttccattaag aatttgtttc gtgctcgaat
     3121 caataccgaa tattaagaaa cattcaagtc tttcttgaaa aaatatgctt gggataattg
     3181 ccaatcaaaa ttaaaagaga ccagatcagc acatttttat ataacccaaa ggaaaactgt
     3241 ttgtaggatt tgcaatttcg ttgtttggcc aataaaatct gttccaaggg ggtaacatga
     3301 ggaaaattat ttcattgttg tcttgttttg ccattgcttt tcaaattttt gtaccggcac
     3361 aaaacacaac tttggcctcc agggtggcat ttgtagatgg ttttgatcta ggggccgagg
     3421 ctggagatgg tgttataaaa cttacctggg agagggtccc gggggcttca ggtggttatt
     3481 atgtttatcg caaggatggt gaaaatggca cttatggggc acccctgact gattttgggg
     3541 tttttgggaa tgttttttat gatggtccga tcccgaatga caacaagtat tgctattata
     3601 ttgctccgct tgatgaaaac tacactgaat ttggtagatc agttgaagcc tgttcctctc
     3661 caatatcaca agcgccgtca tttatcgaac aatgcaagac agtccttgtt tttgttgtcg
     3721 atagtgtgaa ttatttgcac aacggcgtca aaaggattat gagttcacca ttccttctag
     3781 ttggcaggag aacatacctt gctttcaggt atgtaacaga accgcttggc gggaaaatcg
     3841 aatgggatca ggtccagaga aaagtcactg tcacatacaa aagtactgtc gtggagatgt
     3901 gggttggcaa gtcgcaggca aaaatcaacg gcaaaccagt ccagattgat ccaagtgatc
     3961 ccaaggtcgt tccatttgta aaggacggaa ggacctttgt tccactccgt tttccggttg
     4021 aatcttttgg tgaaggcgat gtcaaatggt ttgcccagac aaaacaggca cagcttactt
     4081 tcccggcaga ttgcaatgag aaactgaagg gaaaggttat tagctacaac aacaagacgg
     4141 gcatgtccga gatacttggt tttgaaggcg aatcatacgt tgttgttttc gggcagagtg
     4201 ggtctggcag ttacatacca gttgttggcg actgcatcga gatttcaggc gagaggaaga
     4261 gaaagggaac acacgaatac ttttttgtaa actccatatc cttgatcgaa tgtcaggaag
     4321 ggccatttgc cggatttggg gcagtggaaa aatctgatat tctaagtgct tctgccacta
     4381 tcagttttga gggtggggaa aaaattgatg tcaggataga caaggctcac atcaggatgc
     4441 ttgccggact aaaactggga gattgtgttt tcgcggccgg gatggtcatt aaaaacccat
     4501 ccggtgaaaa atacataaat acaatcattt ttgatatggt gccatgcaag aaaggccaga
     4561 ttgacattgt ttgtgatggc aaatggtatt cggggacagt gcgcgatttt gattgcacaa
     4621 acggaattct gactgttgca ctggatgatg gtcagtcaaa aactgtttca attgagaaca
     4681 gcaattttga ttgcgcaaat ctctcggttg gttattgcac gaggttctgc gcaaaagaag
     4741 tggacggaaa gcttgtttca tcggttttct acgccttccc gtgcggttcc tcttcttgtg
     4801 aaggatccct cataaggggc aaactggtga agattgattg cgcttcgctt agtatgagca
     4861 ttgcacaggg tgataaatac gttgaagtcg cgatatttcc aaaagattgc gaggacatat
     4921 cgtcctcaat gccagaatgc gtggaagctt gcgtagttaa gtctggtaaa cgctttatag
     4981 ctcttaaagt caaaccatac gccgagtgtg aaaatgaatg tcctggggaa actgtccagg
     5041 taaggataga cagggtggat tgcgcggcaa aaattatcta ctgcacaaaa gtggcgacaa
     5101 tggagcaaat ggaaataaac ttcaccttgc ctgaattctg tccactggag gctggaaaat
     5161 gctatgagat ttgcattgaa gagatcaaaa aacccggcca gcaaccggaa agtattgcta
     5221 cgcacttcag gccgatagag tgcaagtaca ggtgtagtag tcaactcgtt actggcacga
     5281 tcaaatcaat tgactgttca aaacaggtcg tagttattag ccagaatgac aatctcgggg
     5341 acataattat aagggtgacc gagcaggatt gtataaggct gaaggaagac cgttgtgtga
     5401 aggcctgtat cacaaaagac ccgaccggtt tatacatggc aaactggatc accgaagtcg
     5461 ggccggaggg atgcacccaa aaaattgatt gcaatggcga cgatttatgg actataagaa
     5521 caacaaaaat agattgcgct ggtaaaaaac ttgaatatga gatgggtgac agaaaaggac
     5581 agctgagggt aaacgattgg tctctgtgca actcgataaa aacaggcctg tgctattcag
     5641 tatgcgtgaa aaa
//
LOCUS       DAOSRI010000095         5630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13098_length_5630_cov_3.101166, whole genome shotgun sequence.
ACCESSION   DAOSRI010000095 DAOSRI010000000
VERSION     DAOSRI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_5630_cov_3.101166"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1243)
                     /locus_tag="PK717_03820"
     CDS             complement(158..1243)
                     /locus_tag="PK717_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR46822.1"
                     /translation="MKKLEQFKDDIYTCVRTRCGFCTQECPTYRDGLIESFASRGKMM
                     QAKGLLEGEIKLSQELAESVFFCAMCGWCEAKCALHNVDIFKALRETLVEAGFTMPGN
                     DLVAKNIIEKGDPLGSGRQTKIEGEVTLYLGCSYRDRPTDTERIVKVLTKLGIKPRIV
                     EETCCANALYNTGYAKEFEKAKENFMKVYGPYINDTIITVCPSCTLTLREYYKLPKVV
                     HASELIRELLPNAQIKKTGSKVTYHDPCHLGRGERMFDQPRDIIKALGYELVEMRLNR
                     EFSQCCGGGGGLQATATDTVKETAKRRIREAMDTGADYITTICPTCESTLFRAALACS
                     REEKEKGTGRKIKSKDIWQLLEEALEV"
     gene            complement(1240..2691)
                     /locus_tag="PK717_03825"
     CDS             complement(1240..2691)
                     /locus_tag="PK717_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR46823.1"
                     /translation="MQDKILEAEKRLKPILGKRVSILEAHRMSYSRDWSPRMYGDDNI
                     PEMVAVPRNTKECQEIVKVAYDLGIPVVAFAGGTGMGGGVQAWKGGIVVDTKGMDQVI
                     EFDEKNMSVRVGAGITIWHLNEFLAKHGMWLPHQPESKQASTVGAAIGCDNDSTFGIK
                     YGKMLECLTSAKVVIGTGEAIEFGHRKASMTSTGYKLHHLFVGSEATLGIVTEAQLRV
                     NYLPKTRSVMGFYVRTIEDACFACEQLMTSGIQLESLHVNDKQRLHFYTHSYRVKYNK
                     EPEVPEWAEAIMFVSVAGDQDVVDFFCAKIRDKVKDIASEVKEQEIVTGYWASKHTLQ
                     FEPFKQKWPDSQRERKFGAADVGVPIGNLEKMHDAFVNLSEKYNQKILGMTVYNECPQ
                     KISPSISFAIFVDDHDQTNMDNFYKFVKEMSLEAIRLEGTMSTYIGDGDRLGGFNQIE
                     HGDSFEYMKKLKDMFDPKNIMNPGKKFESRWIK"
     gene            complement(2691..3440)
                     /locus_tag="PK717_03830"
     CDS             complement(2691..3440)
                     /locus_tag="PK717_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOR46824.1"
                     /translation="MKIKTFLAQGMQKGIENDIVVVVDILRATSNVVTMFDRGATKVF
                     LFEDEEEAKSQKLYYPNMAIAGERNCEKIKGFEMGNSPSETLSAKIKGREVALTTTNG
                     TKAMIQACESETVLVGSIINMTAVCEEIARLVRQTGKDIAVLCSGIDGNPVIDDLYAA
                     GAIINKSIMTGKIRGFDLDDASRVAKMVHGGFSRPLDALLASESGRNLTALGFSSDIA
                     LCAKQDLMNFVPKVKKDDHSYYVVCDDKQEV"
     gene            complement(3494..4732)
                     /locus_tag="PK717_03835"
     CDS             complement(3494..4732)
                     /locus_tag="PK717_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46825.1"
                     /translation="MHIVALLLALLLSVTTTLKAFDIKANVPPEAKRFDVSTIRLFSV
                     LIDNSAGGKIIVYSEGTSREIGEVVTPATQATRASDGFWASHYVCAQNGAKGTIIASA
                     VNAIHIRCGPKRQYDPVKPTNWNASELSIIPFTEEGGTGDIVVSNPGGYGIFNEWSPY
                     VGNPVYALDRGSWVSLDSYFADPTRVPPQFLFIDVRRPRDNVRYIEFENWSKGDVVNG
                     IQMEDYGGVYVMDETEKRYQIGRVLQRATQTGRFIGSEYADIGRVRATHPGVLEVSTT
                     RWRGKTDDENLRGGVQIIPANHAKYLHYNLGQNWFIGGGAWMIVGPVNSTQENLKNPS
                     YTENGKLLIDPIEGLPPIFSGYIRPYFDENNYESSFRFFVSEDFGRTWRTPSEITGVP
                     EAGEKSPVSYWTHVRLMIGK"
     gene            4849..>5630
                     /locus_tag="PK717_03840"
     CDS             4849..>5630
                     /locus_tag="PK717_03840"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR46826.1"
                     /translation="MYAITGTIENLDLAVILQTMNISKKTGCMEVKTGEETSRIFFEQ
                     GAIVHALTKNLEGETAFYDLLTVEQGTWGFIPDMTPPKRTIRNKIEGLLLNWSNLMDE
                     WNSIKDKLPQQDAILRMVDVPEDEREHLVFNMDEWEVLELIKRYGLTSKVFEESKVGR
                     FKTGKAILKFLSIDLIKIEKQDKENIEKIMNFLNDVGSELGERNLGANHFRRIVDKFT
                     VETAKRYKFMNDLRLDENLKFTGLKTKGLLNENVIEALKDLL"
BASE COUNT         1473 a         1387 c         1190 g         1580 t
ORIGIN      
        1 aattttatat gcttccattc ctggaacact ctcggcacca ccagtaataa tttctgtgcc
       61 cctatcatca ccgttatctt ttggctcggg actggcattg ccaccacacg atgcaagcat
      121 caaaacacaa agaactataa gcaatatttt cttcaaacta tacctccaaa gcttcttcaa
      181 gcagttgcca aatgtcctta ctcttgatct ttcttccagt tcccttttcc ttctcttctc
      241 tggaacaggc aagggccgcc ctgaagagtg tggattcaca agttgggcaa atggttgtta
      301 tgtaatcggc gccagtgtcc atggcttccc tgatccttct cttcgccgtc tccttgaccg
      361 tgtcggtcgc tgtggcctga aggccaccac cacctccgca acactgggaa aattccctgt
      421 tgagcctcat ttcgacaagc tcatatccaa gggcctttat gatgtccctt ggctggtcaa
      481 acattctttc gcccctacca agatggcatg gatcatgata agtaactttg gagccagtct
      541 tttttatctg ggcatttggg aggagctccc ttatcaattc agaggcatgg accaccttgg
      601 gcagtttgta atattcgcgg agcgttagtg tacatgatgg gcaaacagtt attatagtat
      661 catttatata tgggccataa accttcatga aattttcttt ggctttttca aattcctttg
      721 catagccggt attgtaaagg gcgtttgcac aacaggtttc ctcgactatc cttggcttga
      781 tgccaagctt tgtgagaact ttaactatcc tttcggtgtc agtcggcctg tccctgtatg
      841 aacaacccag gtaaagtgtc acttcgccct caatttttgt ctgcctgccg gagccaagcg
      901 gatcaccttt ttctatgatg ttcttggcca caaggtcatt gcctggcatt gtaaaacctg
      961 cctccacaag ggtctctctt aaagccttga agatatcaac attgtggagg gcacactttg
     1021 cctcgcacca gccgcacatg gcacagaaga agactgattc tgcaagctcc tgggagagtt
     1081 ttatctcgcc ctcaaggaga ccttttgcct gcatcatctt tcccctagaa gcaaagctct
     1141 cgatgagccc atctctgtat gtggggcatt cctgggtgca gaaaccgcat ctggttctta
     1201 cacaggtgta tatgtcgtct ttgaactgtt ctagtttttt cattttatcc acctgctctc
     1261 aaactttttt ccagggttca taatgttttt cgggtcaaac atgtctttga gctttttcat
     1321 gtactcaaag gagtcgccat gctcaatctg gttgaaacca ccaagcctgt ctccgtcacc
     1381 aatataggtg ctcatggtgc cttccagcct tattgcctcg agagacatct ctttgacaaa
     1441 cttgtagaaa ttgtccatgt ttgtctggtc gtggtcatca acaaagatgg caaaagagat
     1501 tgaaggtgaa atcttctggg gacactcatt gtaaacagtc attccaagta tcttctgatt
     1561 gtacttctct gataaattta caaaagcatc gtgcatcttt tccaggttcc ctatcggaac
     1621 accaacatct gcggcgccaa actttctttc tctctgggaa tcaggccact tttgtttgaa
     1681 tggctcaaac tggagcgtgt gttttgaggc ccaatagcca gtcacaatct cctgctcctt
     1741 tacctcagag gctatgtcct tcactttgtc ccttatcttg gcacagaaga aatcgacaac
     1801 atcctggtcg cctgctacag aaacaaacat aatcgcttct gcccattccg ggacttcagg
     1861 ctccttattg tatttgaccc tataagagtg cgtatagaaa tgaaggcgct gtttatcgtt
     1921 gacatgcagt gattcaagct ggatacctga cgtcatcagc tgttcgcatg caaagcatgc
     1981 atcctctatg gtgcgaacat aaaatcccat tacagacctt gtttttggca gatagtttac
     2041 cctgagctgc gcctcggtaa caataccaag cgtggcctcg gaaccaacaa aaagatgatg
     2101 gagcttgtac ccagtagaag tcatgctggc cttgcgatga ccaaactcaa ttgcttcgcc
     2161 agtgccaata acaactttgg ccgatgttag gcactcaagc atttttccat acttgatacc
     2221 gaaggtcgaa tcattatcac agccaatggc tgcaccgact gttgaggcct gtttggattc
     2281 gggctggtgg gggagccaca tgccatgctt tgcaaggaat tcattgaggt gccatattgt
     2341 aatgccggca cccaccctga cagacatgtt tttttcatca aattcgatga cctgatccat
     2401 acccttggtg tcaactacta taccgccctt ccaggcctgg acaccgccac ccattccggt
     2461 ccctccagca aaagcaacta cagggatgcc aagatcgtaa gcaaccttga cgatttcctg
     2521 acactctttt gtgttccttg gaactgcaac catctccggg atgttgtcat caccatacat
     2581 ccttgggctc caatccctgc tgtagctcat tctgtgggcc tcaagtatgg aaaccctttt
     2641 gcccagaatg ggttttagcc gtttttcagc ttccaaaatt ttgtcctgca tcagacctcc
     2701 tgtttgtcgt cgcaaacaac atagtatgaa tgatcatctt tttttacctt tggaacaaaa
     2761 ttcataagat cttgctttgc gcaaagcgca atgtcagagg agaagccaag ggcagtgagg
     2821 ttcctgcccg attcagaggc aagaagggca tctagcgggc gcgagaagcc cccatgcacc
     2881 atttttgcga cccttgaggc atcatcaaga tcaaaacctc ttatcttgcc tgtcattatt
     2941 gatttattga ttatagcgcc agcggcataa agatcatcga tcacagggtt cccatcaata
     3001 cccgagcaca gcacggcaat atcttttccg gtttgtctga caagccgtgc gatctcttcg
     3061 cagacagcag tcatatttat tatcgagcct acgagcactg tttcggattc acatgcctgg
     3121 atcatggcct tggtgccgtt tgttgttgtg agcgccactt ccctgccctt tatctttgcg
     3181 gagagagttt ccgagggtga attgcccatc tcgaaccctt ttattttctc gcaattcctc
     3241 tcgccagcaa ttgccatgtt cgggtaataa agcttttgtg atttcgcttc ttcttcatct
     3301 tcaaaaagga acaccttggt ggcgcctctg tcaaacatcg tcacaacatt gcttgtggcc
     3361 ctcaaaatat caaccacaac aacaatgtcg ttttctatcc ccttttgcat tccttgagcc
     3421 aaaaaagttt ttatcttcac aaaaaactag tttattattg tgaaagttta agttcaacca
     3481 cggacatgag cagctacttg ccaatcatga gcctgacatg cgtccagtaa gaaacagggc
     3541 tcttctctcc tgcttcggga acgcctgtta tttcagaggg agttctccac gtccttccaa
     3601 aatcttcgga aacaaaaaat ctgaaagaag actcataatt attttcatca aaatagggtc
     3661 ttatataccc agaaaagatt ggtgggagac cctcaatagg atcgatcagc aacttcccat
     3721 tctctgtata tgaaggattt ttgaggtttt cctgtgtaga attgacggga ccaactatca
     3781 tccaggcacc gccaccgata aaccagtttt gtcctagatt gtaatgaagg tatttcgcat
     3841 ggttcgctgg aattatctgt acaccacccc tcaaattctc gtcatcagtc ttgcccctcc
     3901 atcttgtggt ggaaacttct agtacacctg gatgggtagc ccttaccctt ccgatatcgg
     3961 catattctga accaataaac ctgccagttt gggttgccct ctggagaact cttccaattt
     4021 ggtacctttt ctcggtttcg tccatcacat agactccacc ataatcttcc atctggatac
     4081 cgtttacaac atcgcccttt gaccaatttt cgaattcaat gtacctgaca ttgtcccttg
     4141 gtctcctgac atcaatgaac aaaaattgcg gcgggacgcg tgttggatct gcaaaatatg
     4201 aatcaagcga cacccaggaa cctctgtcca gggcgtaaac ggggttgcca acataaggcg
     4261 accattcatt gaaaatcccg tatcctccag gattgcttac tactatatcc cctgttccac
     4321 cctcttcggt gaatgggatt attgaaagct cggacgcgtt ccagtttgtc ggtttcacag
     4381 gatcatactg tctttttggc ccgcatctga tatggatcgc gtttactgca gatgcaatga
     4441 ttgttccttt ggcaccattt tgtgcgcaaa cataatggga tgcccagaaa ccatccgagg
     4501 cccttgtggc ctgggtggca ggagtaacaa cttcccctat ctcccttgag gtaccttcag
     4561 aataaacaat tatttttcca ccagcactgt tgtcaatgag cacggaaaaa agtcttatcg
     4621 tagaaacatc aaaccttttg gcctctggcg ggacattggc tttaatgtcg aaagccttca
     4681 gcgttgtggt cacagacaaa agcagggcca gaagaagcgc cactatgtgc attgggtaaa
     4741 tcataacaag actctagtaa tacctcaaga aaacatttag ccacaataaa tgttcagctt
     4801 gacaacaaat caatagtaaa ttaacttatc tgtttgcgga ggtgcgcaat gtatgccatt
     4861 accggaacta tagaaaacct tgatttagca gtaattctgc agacaatgaa tataagtaaa
     4921 aaaaccggtt gcatggaagt aaagaccggt gaagaaacct cccgcatttt ttttgagcag
     4981 ggtgcgatag tgcatgcgct cacaaaaaac ctcgagggtg agacggcttt ttatgaccta
     5041 ttgacggtag aacagggcac atggggtttt atccccgaca tgactccgcc aaagaggaca
     5101 ataaggaaca agattgaggg gctactcctt aactggagca acctcatgga cgagtggaat
     5161 tcaatcaagg acaaactccc ccagcaagac gccatcctga ggatggtgga tgttcctgag
     5221 gatgaaaggg aacacctggt tttcaacatg gatgagtggg aagtcctcga gctgataaaa
     5281 aggtatggtt tgacatcgaa agtattcgaa gaaagcaagg taggaagatt caaaacaggc
     5341 aaggcaatcc tgaagtttct ctcaatagat ttgataaaga ttgagaaaca ggacaaagag
     5401 aacattgaaa aaatcatgaa cttcctcaat gacgtgggga gcgaactggg tgaaagaaac
     5461 ctgggcgcaa accacttcag gcgcatagtt gacaagttca cagtagagac ggcaaagcgc
     5521 tacaagttca tgaacgactt gaggcttgac gaaaacctta aatttacagg ccttaagaca
     5581 aaaggactgc tgaacgagaa tgttatcgaa gcactgaagg acctgctatg
//
LOCUS       DAOSRI010000096         5629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13101_length_5629_cov_4.171869, whole genome shotgun sequence.
ACCESSION   DAOSRI010000096 DAOSRI010000000
VERSION     DAOSRI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13101_length_5629_cov_4.171869"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /gene="lepB"
                     /locus_tag="PK717_03845"
     CDS             complement(<1..429)
                     /gene="lepB"
                     /locus_tag="PK717_03845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR46827.1"
                     /translation="MTVKKSFKREILEWVVTIVIALGVAWSIRLFVIQPFRVQMSSMY
                     PTLHNDDLILINKLSFVGTQPGRGDIIVFTPPNHAEGDGIEYIKRIIGLPGEYIEIES
                     GTVYINGHKLEENDDHTKTNYNQHFNVESDSDKKGILLGPD"
     gene            complement(429..791)
                     /gene="rplS"
                     /locus_tag="PK717_03850"
     CDS             complement(429..791)
                     /gene="rplS"
                     /locus_tag="PK717_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR46828.1"
                     /translation="MNEILREVVQDQIKKDPNSYEIGDTVKVHQKVKEGTRARIQIFQ
                     GIVIAKQNGGVSETFTVRKISDGVGIEKIYPLHSPAIDKIEVVRHGRVRRAKLFYLRD
                     RIGKEARLKDRKPQQKKA"
     gene            complement(932..1483)
                     /locus_tag="PK717_03855"
     CDS             complement(932..1483)
                     /locus_tag="PK717_03855"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR46829.1"
                     /translation="MIFISLMHHPVYDRRGNIQTSSITTFDIHDLARSSTTYGVKRLY
                     LVHPSPLQRQVAERILGFWEVGGGKEWNPFRSQALEVTRVTANLDMAIEDATQIAGEK
                     PVLVGTTAKTKEGQVTFEWLRNNLDRPTMIILGTGWGLSPEIINRCDLLLEPIWGPTE
                     FNHLSVRAAGAIICDRLLGKRLV"
     gene            complement(1480..2232)
                     /gene="trmD"
                     /locus_tag="PK717_03860"
     CDS             complement(1480..2232)
                     /gene="trmD"
                     /locus_tag="PK717_03860"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR46830.1"
                     /translation="MMKLVFATTFPDFFKESAKVSIMGRATKAGLLEVEAINLRDFTT
                     DRHRTTDDSPFGGGAGMVLKVDPIKKMLDSLGLPEGSRTILTTPAGKAFDQQVASDLS
                     KAPALIFLCGHYEGIDERACQLFTDWLSIGDFIMTGGEIAALAMADATVRLIPGVLGK
                     QDSLEFESFSDNLIEYPQYTRPAQHETGDVPEELQSGNHKTIEKWRRTQALKRTLLLR
                     PDLLIDANLSKEDIETLEEIKTEILDTIGKIK"
     gene            complement(2229..2633)
                     /locus_tag="PK717_03865"
     CDS             complement(2229..2633)
                     /locus_tag="PK717_03865"
                     /inference="COORDINATES: protein motif:HMM:NF022519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlqD family protein"
                     /protein_id="HOR46831.1"
                     /translation="MLIARKVALKVIVTDDFKKRYVAQLNSLSAEVKVELEKIKTTEA
                     QLMLKVQTMDYNYVMQVREQLEREKSTRESTLKEIESRTAEMQGMAEGTIFPQGTLDS
                     LVEIKSGDDLELKLSKAEIIIKDNVVQSVNEG"
     gene            complement(2637..2873)
                     /locus_tag="PK717_03870"
     CDS             complement(2637..2873)
                     /locus_tag="PK717_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOR46832.1"
                     /translation="MFKDTLLYIVKQLVDNQDKVVINELVGEQHLTYEIKVDPSDLGK
                     VIGKQGRTINALRVLIRAVSNKHGKKAEVNVIEA"
     gene            complement(2882..3160)
                     /gene="rpsP"
                     /locus_tag="PK717_03875"
     CDS             complement(2882..3160)
                     /gene="rpsP"
                     /locus_tag="PK717_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOR46833.1"
                     /translation="MAAKLKLARVGKVHSPSFRLIVVDSRKPTNSDFIELVGHIDFVH
                     DNKVTNLKEDRILYWLSVGAQPTESAKSILKTSGIMRKFAESKRKVQV"
     gene            3299..4780
                     /locus_tag="PK717_03880"
     CDS             3299..4780
                     /locus_tag="PK717_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46834.1"
                     /translation="MLKNKQLMITLAIILIAIVVGVSVVLAIFFTRERGLGIMPYEYA
                     PADTFVYAQFSTGTNMGDVTNNILPLVSSSLPVVPQEVLSLIKDLPDLSFLNLSGFVT
                     YRNDGLLVGISLSHRTNSEKAFDNIIEYLKKQEITAKKSESGIVEFSNKNMPGSMLAM
                     KLESDYLLIGSPSSNPESSARKIIEDALLTKKDEKGIQTLKEWDEIVEAMGGKISNLG
                     FYLTDRKNENPVKIAGKLYSQDGESGIDAQLIGSVDSEIEKMDPLYRDSIKAMLAGQM
                     PFDGLRSSVLDNGIIQAAISAGLGASKIGTEELNDKETALSFVNGKTALWVDINAESS
                     KTSFGISTIASVQNVKSFLEFLFPQDRYLRKNEKGIFSVWETMVGEDGKKVESLEPVS
                     YMKFEDAGNGNQKLVISSEKDGLGRFTKEQPAKTAENCIVDVGVNVDKLPIDNNLGIK
                     KAFDMLKPIGLKIGAKSFVKNDRLHARVFVTYDENKVRSILNK"
     gene            4836..>5629
                     /locus_tag="PK717_03885"
     CDS             4836..>5629
                     /locus_tag="PK717_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46835.1"
                     /translation="MAKKLLTILTVLALVPTMFWFEVKAAVAPWMMYGFSAEHKFASP
                     ESLNFPLSLLWQYNSGGGFACKAGPCSHNNTFIVPLASGVVECMDSTQGKMLWSKKLS
                     SLPIVSRPAASGDYVLVTTQEGILYSLLHKNGEIVAKFDAGDHITTSPLIVGGLVYLA
                     TTGGIVIRLTLNGLYFWGASDVGGQIKLDPVVTQSQGKNMVIVATKEGPINCLSPFPS
                     VAWTIKPADPVEGSIAAIDDGVLYMTSKGILCNATAPDGSLVWSLP"
BASE COUNT         1410 a         1256 c         1336 g         1627 t
ORIGIN      
        1 atccgggcca agaaggatac cttttttatc gctgtcactc tcaacattaa aatgctggtt
       61 ataattggtt tttgtgtggt cgtcattttc ttcgagcttg tgcccgttga tatagacagt
      121 accagactca atttcaatgt attcgcccgg caagccaatt atacgcttaa tgtactcgat
      181 gccatcacct tcagcgtggt tgggcggcgt gaagactatg atatcgcccc tcccaggctg
      241 ggtaccgaca aaagacagtt tgtttatgag gatgaggtca tcattgtgca atgttgggta
      301 catggaactc atttgcactc tgaaaggctg gattacaaag agccttatgc tccaagcaac
      361 accaagcgcg atgacaattg tcaccaccca ttcaagtatt tccctcttga agcttttttt
      421 gacggtcatc aagccttctt ttgctgtggt ttgcggtctt tcagtctggc ttctttgcct
      481 attctgtccc tgaggtagaa cagttttgct cttctcactc ttccgtggcg tacgacttca
      541 atcttgtcga ttgctggtga gtggagggga taaatcttct ctattccaac gccgtcggag
      601 atcttcctga ccgtgaaagt ctcggaaaca ccgccgtttt gtttggcgat gacgatgccc
      661 tggaaaatct ggatcctggc ccttgttcct tcttttacct tctggtggac cttgactgtg
      721 tcccctatct cgtaggaatt tggatccttc ttgatctggt cttgtacaac ttctctcaaa
      781 atctcgttca tatttatcct ccaaaggccc tttgggactc gcccttcagg ctttccaatg
      841 caatacaata acgtaaagca cctagaaagg agatgttagc cgtcctttgc agtgtttgca
      901 agtgaaaata gtttaatgaa aaaaaagttg attaaacgag tctttttcca aggaggcggt
      961 cacaaattat tgctcctgcc gccctgactg acaggtggtt gaattctgtt ggcccccaga
     1021 ttggctctaa aaggagatcg catctgttta ttatttctgg cgaaagcccc cagccagtgc
     1081 cgaggattat cattgttggc ctgtcgagat tgttcctcag ccactcaaag gtcacctggc
     1141 cttcttttgt tttggcagtt gtgccaacaa gcaccggttt ttctccggcg atctgtgtgg
     1201 cgtcttcgat ggccatgtcg aggttggcag tgacccttgt tacctcaagg gcctgtgacc
     1261 tgaacgggtt ccattctttc ccaccgccga cttcccagaa tcccaaaatg cgctctgcga
     1321 cttgtctctg aagtggggat ggatggacaa gatagagcct ttttacgccg taggttgtgg
     1381 agcttcgcgc gaggtcatga atgtcgaaag tggttattga agaggtttgt atgttgcccc
     1441 ttctgtcata aacagggtgg tgcataagtg atataaaaat cattttattt ttccaatcgt
     1501 gtcaagaatt tcagttttta tctcttcgag tgtttcaatg tcttcttttg aaagattggc
     1561 gtcaatcagc aggtcggggc ggagaagcag tgttcttttt agtgcctgtg tccttctcca
     1621 tttttcgatc gttttgtggt tgcctgattg gagctcttct gggacatcac ccgtttcatg
     1681 ttgggccggc cgtgtatatt gagggtattc aatcaaattg tcagagaaag attcaaattc
     1741 gagggaatcc tgtttgccga gaacaccagg gataagcctc actgttgcat cggccattgc
     1801 aagggctgca atttcaccgc cagtcatgat gaagtcaccg attgatagcc agtccgtaaa
     1861 aagctggcaa gccctctcgt caatcccctc ataatgtcca cataaaaaaa ttaaggccgg
     1921 ggcttttgag aggtcgcttg cgacctgttg gtcaaaagcc tttccggccg gtgttgtgag
     1981 tattgtcctg gatccttctg ggagccccag tgaatccagc atttttttga tagggtcaac
     2041 cttgagcacc ataccggccc cacccccaaa agggctgtcg tcggttgtgc ggtggcggtc
     2101 tgttgtaaag tctctgaggt tgattgcttc aacctcaagc aaacctgctt tggtggccct
     2161 tcccattatc gaaacttttg ccgactcctt gaaaaaatca ggaaaggtgg tggcaaaaac
     2221 aagcttcatc agccttcgtt tacgctctgg accacgttgt ctttaatgat gatttcggct
     2281 tttgagagct ttaattccag gtcgtcgcca gatttgattt caacaagcga atccagtgtt
     2341 ccctgcggga aaattgtccc ttcggccata ccctgcattt cggccgttct ggactcaatc
     2401 tccttgagcg tggattccct tgtggacttc tccctttcga gctgctccct gacctgcatg
     2461 acataattgt aatccatggt ctggaccttc agcatgagct gtgcctcggt tgtcttgatt
     2521 ttttcaagct ccaccttgac ctctgctgaa agggaattca gctgtgcgac atacctcttc
     2581 ttgaagtcat cggtgacgat gactttcagt gcaacttttc tagctatcag cacgaattaa
     2641 gcctcgatta cgtttacttc ggccttcttg ccgtgtttgt tggatacggc cctgatgagg
     2701 acgcggagag cgttgatcgt cctgccctgc ttgccgataa cctttccaag atcacttggg
     2761 tcaaccttga tttcgtaggt aaggtgctgt tcgccgacga gctcattgat gactactttg
     2821 tcctggttgt ccacgagttg cttgacgatg taaagaagag tgtctttaaa cattgttccc
     2881 cctaaacttg tactttgcgt ttggattcgg caaatttcct catgataccc gaagttttaa
     2941 ggattgactt ggcagattcg gtgggttgtg cacccacgga gagccaatag agaatcctgt
     3001 cttcttttaa gttggtgacc ttgttatcat ggacaaagtc aatatgccca acaagctcta
     3061 tgaagtcgct gtttgtgggt ttgcgggagt caacgactat gagtctgaag ctggggctgt
     3121 gaactttacc gacgcgagcg agctttagtt ttgcagccaa tttgaaaaac ccctttctat
     3181 tacttcttac aaagtaccga ttttagggat gggactaccc ctgtcaaggg gggttgctaa
     3241 gatttcgcaa aggaattata tatcatcttt acgtttcaaa tctggaggtg cgttttttat
     3301 gctgaagaac aagcagttaa tgataacact tgccattatc cttatagcca tagtggttgg
     3361 agtaagtgtt gtattggcaa tattttttac aagagaacgc ggcctgggga tcatgcccta
     3421 cgagtatgca cctgcagaca cctttgttta tgcccaattt tcgacaggca ccaacatggg
     3481 tgacgtaact aataatatat taccactggt ttcctcttcg ttgccggtgg tgccgcaaga
     3541 ggtattgtca ctgataaagg atcttcctga cctctctttt ctcaatctat ccggatttgt
     3601 gacatacagg aacgatggtt tactcgtagg aataagtctt tcacatagaa caaattccga
     3661 aaaagcgttt gacaacatta ttgaatacct aaaaaaacag gagatcacag ccaagaagag
     3721 tgagagcgga atagtagagt tttcaaacaa aaatatgcca ggcagcatgc tggcaatgaa
     3781 gctcgagagc gattatctct tgattggcag ccccagcagc aatcctgaat cttcggccag
     3841 aaaaatcatt gaagatgccc ttttgacaaa aaaagatgaa aagggaatcc aaaccttgaa
     3901 ggaatgggac gaaatagtgg aggcaatggg tggaaaaatc tccaatctgg gtttctacct
     3961 gacagacaga aagaatgaaa atcctgtgaa aatagctggc aaactctaca gtcaggatgg
     4021 ggaatcaggc attgatgcac agcttattgg cagtgttgac agcgagatcg aaaaaatgga
     4081 tccattgtac agggattcca taaaggctat gcttgcaggg cagatgccgt ttgatggcct
     4141 caggtcttct gttttagaca atggaattat tcaggccgca atatctgcgg gtcttggagc
     4201 gagcaaaatt ggcacagaag agcttaacga taaggaaaca gcgctctctt ttgtgaatgg
     4261 aaagacggcc ctgtgggtcg acatcaatgc agaatcttca aaaaccagtt ttggcataag
     4321 cacaatcgcc agtgtccaga atgtaaaatc tttcctggaa ttccttttcc cgcaagatag
     4381 atatttaaga aaaaatgaaa aaggtatctt ctcggtttgg gagacaatgg ttggagagga
     4441 tggcaaaaaa gtggaatctc ttgagccggt ctcttacatg aagtttgaag atgccgggaa
     4501 tggaaaccag aagcttgtaa tctcgagcga aaaagatggt ttggggagat ttaccaagga
     4561 acagccagca aaaactgcag aaaattgtat tgttgacgtt ggtgtaaatg tcgacaaatt
     4621 gcccatagac aataatttgg ggataaaaaa ggcctttgat atgttgaagc cgattggcct
     4681 gaaaattggg gcaaaatctt ttgttaaaaa tgacagactc catgcaaggg tatttgtgac
     4741 ctatgatgaa aacaaagtca ggagtattct taacaagtag cctcttgctt gacctaaggt
     4801 aaaaaatctg taaattttaa ctcgaaggct aagctatggc aaaaaaactg ttaacgatac
     4861 taacggtttt ggcacttgtc ccaaccatgt tttggtttga ggttaaggct gctgtagcgc
     4921 catggatgat gtatggattt tcggccgaac acaaattcgc ttcccctgaa tcgcttaact
     4981 tcccactcag cttgctctgg caatacaact caggtggtgg cttcgcctgc aaagctggcc
     5041 catgctcaca taacaatacc tttatagttc cgcttgcatc aggtgtggtc gagtgcatgg
     5101 actcaacgca gggcaaaatg ctttggtcaa aaaaactctc ttcattgccg attgtttctc
     5161 gtcctgccgc ttctggagac tatgttctgg tgacaaccca ggagggaata ctctacagtc
     5221 tcctgcacaa aaatggcgag attgttgcca aatttgatgc tggggatcat ataacgacgt
     5281 ccccgctcat agtcggtggt cttgtgtatc ttgccacaac cggtggcatt gtgatccggt
     5341 tgacactcaa cggactctat ttctggggcg cctcggatgt tggtggccag ataaagcttg
     5401 atcctgtagt tacccagtcg cagggcaaaa acatggttat tgtggccaca aaagaaggcc
     5461 ccataaattg tctttcacca ttcccgtcag ttgcatggac gatcaaaccg gcagacccgg
     5521 tagagggttc aattgcggcc atcgatgatg gcgtcctgta tatgacatca aaaggtatcc
     5581 tctgtaacgc cacagctcca gatggttccc tcgtatggtc actccccca
//
LOCUS       DAOSRI010000097         5612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13149_length_5612_cov_3.754364, whole genome shotgun sequence.
ACCESSION   DAOSRI010000097 DAOSRI010000000
VERSION     DAOSRI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13149_length_5612_cov_3.754364"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PK717_03890"
     CDS             complement(<1..867)
                     /locus_tag="PK717_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46836.1"
                     /translation="MPKKHLPIYKHAILLLVGTTLLLFPKNTGSNNTLPTKKVIFKSE
                     YMINNPFINSRYIVWSQHIKDESGITYKILSFDTITGNLIEVATDDSLMEMPGDNNIY
                     LGIAHCCWINIEGKGANAFVIKGSKVYNNRSEIKISKGRLPWKNANVYNSYVTYVEAN
                     DENLCSYVVDLSEIQPKYILLTQKARVSTTPRLSHNQVVYEATDDDGNPSIFLYDLDR
                     GTSLKISQDTFLASNPHVTKNYVYYLSSSTSNDTVNVMCYDIKNHEYTNIYQLKKGLH
                     ISLINNPDSDLLM"
     gene            complement(999..1748)
                     /locus_tag="PK717_03895"
     CDS             complement(999..1748)
                     /locus_tag="PK717_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46837.1"
                     /translation="MKKIAIFLAIASLAVGASWIPQARADGTSEIISNLPGDAQCQFE
                     VWWRKDPGTTIVNEGKTATFKFGITNWEYPDKMHFVITTSDKNIQFQKTDFYSNETFM
                     EMTITITMPKRQSGQKVAYWSFKITSDCGRHYSVKFHVHYPEECCSYKTSWEKEPASF
                     KVAQNKTCSFKFVVENKCSEAPIDFQLSSNISSLGFSRKSFTVQPGKKFEITVTIKQP
                     SQGGKSKSDWSFKIKPACGQEKQISFSVKYP"
     gene            1918..2895
                     /locus_tag="PK717_03900"
     CDS             1918..2895
                     /locus_tag="PK717_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46838.1"
                     /translation="MENSKNISWEIKVPLLANTAILTDILKGFGLTYLIVVLLMGFIF
                     LLQGEMEGFLQIVWMFALIVGGLMVLSALVMLIVFRNSYHIKYTLDSEGATFETLDRR
                     VKMANRVAIGAGFLGGGQAAGAGLLARSQETIYVPWKNINQVLYNEKRGRITLRNTWR
                     NVMIIFTGRGPWQEAANAVRENVREASAEKPAKKGNALVKMLLATVFVVACVAATFAV
                     PFETELFVKILVLCFALATVWLSRLMAYPVLLGLAYMIYEILVQGFEVSTWTLVSVHT
                     STGFGFLSGDEWAALVLGFLGIAGLAIYCILSLTGRTSSALEGDEAGEN"
     gene            2914..4233
                     /locus_tag="PK717_03905"
     CDS             2914..4233
                     /locus_tag="PK717_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46839.1"
                     /translation="MKKMSRGLSLFGVIFLFCSSCSFAKPYEVKLVSDKRPPGKIIGI
                     LSDEGKVHRELFFPILVNDQEVTTYKDPLLQQPVKTINLPGEVLSYSLKNNSTTTTES
                     GGYHNSEYDKNYLVFLCRGNTSNTIVLYQFDYSNLIKGNANETKSASFSTKADKIISI
                     SHNDKDHKALLVCQSNDNKLLYLSDPWGKTGHIKSLESPMLFADLRYNEFSPTSPTMV
                     GYDGKIVYFYKFVDGQLLVDFKIDLGTEGDLTKSVINDLNSLEGYGYGWLFSLYDGKA
                     IHLIDRFNKSVIYSREFLDTTFTKQLDYEFYFGSNAMFYISTKRVFDSRMNNAGDFIA
                     SRLFEVDYKPLSYVRTPDISLYYFCQYNPDSTLVYDMQTVRDNQNRLFRKHSYREIPG
                     KVVNVLFLSYKIYFYTEDSVWECDYMNTAEGQYDLIKMDLGPEYEEK"
     gene            complement(4299..5444)
                     /locus_tag="PK717_03910"
     CDS             complement(4299..5444)
                     /locus_tag="PK717_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46840.1"
                     /translation="MKKFAVLTVLFMVLALPLIGCGSGDGANSGGTDTGKQVATHQDW
                     PMMMGNMSRTGTATGGISAQPAIEKAWEFESKEPLRGQPVASNGKAYFGSDKLYCLDI
                     PTGKEEWSFQADAKIWNTPCISGEKIYFADEAGKFYCVNTRSKNPVWTSKFSSKIESS
                     PAVSSGVAFFGDMGGNFHCLNADSGYEYWSFKAGRGIASAPALLDGKVYFGSQDQKLY
                     CLNQETSELVWESKTDDGIVNPVVASSGKVWAIVGDANLVCFDALTGAKLWTFKSGDK
                     VTAPAVYDQFVFTGSDLDTFYCLNIADGKEVWNAPVGDNVFGSPAICGDKVFFGSDNK
                     TLYCMDAKTGKELAKLDCGYPSNANIAISDGTIITCTDGKVLCFTIR"
BASE COUNT         1455 a         1207 c         1270 g         1680 t
ORIGIN      
        1 catgagtaga tcactatcag ggttatttat taggctaata tgaagcccct tttttaattg
       61 ataaatattg gtgtattcat ggttcttaat atcatagcac attacattta cagtatcatt
      121 tgaagtggaa ctggataaat aatagacata attttttgta acgtgagggt tagatgcaag
      181 aaaagtatct tgtgatattt tcaaagaagt acccctgtct agatcatata gaaatataga
      241 aggattacca tcatcatcag tagcttcata tactacttgg ttatgggata acctgggtgt
      301 ggtagaaacg cgtgcttttt gagtaagcaa aatgtatttt ggctgtattt ctgataaatc
      361 aacaacgtac gagcacagat tttcatcatt tgcttcaaca tacgtcacat agctgttata
      421 aacattggca ttcttccaag gcagtcttcc tttggagatt tttatttcag aacgattatt
      481 ataaaccttg ctacccttta ttacaaacgc attggctccc tttccctcaa tattaatcca
      541 acaacaatga gctatcccca gataaatgtt gttatcgccc ggcatctcca tcaaagaatc
      601 atcggtagcg acttctatta agttgcctgt aattgtatca aaagataata ttttgtaagt
      661 gatgccgctc tcgtctttga tatgttggga ccagacaatg tatctagaat taataaatgg
      721 attatttatc atatattcag atttgaatat cacttttttt gtgggtaatg tattattgga
      781 acctgtattc ttcggaaata gtaatagtgt tgtcccaact agaagtaata ttgcatgctt
      841 atatattggt aaatgttttt ttggcattag ttattgtcgc gccacctcaa agggcaatat
      901 acaacaatcc agattttata acaataagat cttaccctcc ctccaaaaat aaaaaaagcg
      961 gggcaaaacc ccgctttttc caccaagtct ctttgaaatt atggatattt tacactaaaa
     1021 ctgatctgct tttcctgccc gcatgccggt tttatcttga agctccagtc cgattttgac
     1081 ttgccaccct gggacggctg tttgatggtc acggtgattt caaacttttt cccgggctgg
     1141 acagtgaagc tttttctgga aaaaccgagt gatgatatgt tgcttgagag ctggaaatcg
     1201 attggggcct cgctgcattt gttttccacc acaaatttga acgagcaggt tttgttctgg
     1261 gcaaccttga agcttgcagg ctccttttcc caggatgtct tgtaggagca gcattcctcc
     1321 gggtagtgga catggaattt gaccgaataa tgcctgccac agtcggaggt gatcttgaag
     1381 ctccagtagg cgaccttctg gccagactgc ctctttggca ttgtgatcgt gattgtcatc
     1441 tccataaaag tctcgtttga gtagaaatcg gtcttctgga actggatgtt tttgtctgat
     1501 gttgtgatga caaagtgcat tttgtccgga tattcccagt tggtgatccc aaacttgaac
     1561 gttgctgttt tgccttcatt gacgattgtt gtccccgggt cctttctcca ccagacctca
     1621 aactggcatt gtgcgtcccc tgggaggttg cttattatct cgcttgtccc gtctgctctt
     1681 gcttgtggta tccatgaggc gccaactgcc agactggcaa tggccaaaaa tattgctatt
     1741 ttcttcatct tctgcctcct tgtgttattt gatttcttat acggggcgtt ttggcgtttt
     1801 gtctgttgcg ccttcccgtg cttgctctgg ccagatttgt tcccaaaaag tttactttcc
     1861 ttgcgggctg ggctatcatt tttttagaaa acggccacaa aaacaaagga gcagataatg
     1921 gaaaacagca agaatatttc gtgggaaata aaggtgccac tcctggccaa cacggcgata
     1981 ctcaccgaca tactcaaggg ttttggtctg acctatctca tcgttgtttt gcttatgggc
     2041 tttatttttc tgctccaggg cgaaatggag ggatttctcc agattgtctg gatgtttgcg
     2101 ctgattgtcg gcggtctgat ggtgctttct gccctcgtca tgctgattgt tttccggaac
     2161 tcctaccaca taaaatacac gcttgattcc gagggcgcca ccttcgaaac gctggacaga
     2221 agggtgaaaa tggcaaacag ggtcgcaatc ggcgccgggt tcctgggcgg tggccaggcg
     2281 gccggcgctg ggctccttgc cagatcgcaa gagacaatct atgttccgtg gaaaaacatc
     2341 aaccaggtac tctacaatga aaaacgcggc aggataacgc tccgcaacac atggcgcaat
     2401 gtcatgatca tctttaccgg aagaggcccc tggcaggagg ccgcaaatgc tgtcagggag
     2461 aatgtcaggg aggcatccgc agagaagccg gcaaagaagg gcaatgcgct tgtcaaaatg
     2521 cttcttgcga cagtctttgt tgtggcatgc gtcgcagcaa catttgccgt gccttttgaa
     2581 acagagcttt ttgtgaagat ccttgtcctg tgctttgccc ttgccacggt ctggctctca
     2641 aggctgatgg cttacccggt tttgcttggg cttgcgtata tgatctacga aattttggtg
     2701 cagggttttg aagtttcaac gtggacacta gtaagcgtcc acacctcgac aggcttcggg
     2761 ttcctctccg gagacgagtg ggccgcgctt gttctgggct ttctgggcat tgccgggctg
     2821 gcgatatact gcatcctttc tcttactgga aggacaagtt cagcgcttga gggcgatgag
     2881 gccggggaga actaatatat tattggagac aaaatgaaaa aaatgtccag gggattatct
     2941 ttgtttgggg ttatattttt gttttgctca tcttgttcct ttgcaaaacc atatgaggtc
     3001 aaacttgtgt ccgataagag acctccaggg aaaataattg gtatcttgag cgatgaggga
     3061 aaggttcatc gtgaattgtt ttttccaata ctcgtaaatg accaagaggt aacaacatac
     3121 aaagatccac tgttgcagca accagtcaaa acaatcaacc tgcctggtga agttttgtca
     3181 tattccctaa aaaataactc aacaaccacg acagaatctg ggggatatca caatagtgag
     3241 tacgataaaa actatttagt attcttgtgt cgcggcaaca cctctaatac aattgttcta
     3301 tatcagtttg attattctaa tctaataaaa ggtaatgcaa acgagacaaa atcagccagt
     3361 ttctcaacaa aagctgataa aataatatca atttctcata atgacaagga tcataaagct
     3421 ctgcttgtct gccagtcaaa tgacaataag ttgctttatc taagtgatcc gtgggggaaa
     3481 acagggcata taaaatctct tgaatcacca atgctttttg cagatctgag atacaacgaa
     3541 ttcagcccta cgtcaccaac aatggtcgga tacgatggca aaattgttta tttttacaag
     3601 tttgttgatg gccagttact tgttgatttt aaaatagacc tgggaacgga aggtgattta
     3661 acaaagagtg taattaatga tttgaatagc cttgagggat atggttatgg ttggcttttc
     3721 agtctgtatg atggtaaagc gatccatctt atagatagat tcaataaatc agtaatttat
     3781 tcaagagagt tcctggatac aactttcaca aagcaacttg attacgagtt ctattttggt
     3841 agtaatgcta tgttttatat aagtactaag cgtgtatttg acagccgtat gaataatgct
     3901 ggagatttta ttgcaagtag attgttcgag gttgattata aaccattgtc atatgtacgg
     3961 actccggata tcagtcttta ttacttttgc caatacaatc cagattccac gttggtctat
     4021 gatatgcaaa cagtccggga taaccaaaat agattgttta gaaaacattc ttacagggaa
     4081 atccctggaa aagttgtaaa cgttctgttt ctctcataca aaatctattt ttacacagaa
     4141 gattctgttt gggaatgtga ctacatgaat acagcagaag gccaatacga tcttatcaag
     4201 atggatcttg ggccagaata tgaagaaaaa tagtttgata aatgacaaag ccccctcggt
     4261 aatcgaggag gctttgtcca aaagaacaga atagggtctc acctgattgt aaagcagagc
     4321 accttcccgt cggtgcaggt gatgatggtg ccgtcagaga ttgcgatgtt tgcgttgctc
     4381 ggatagccgc aatccagctt tgcaagctcc ttgccggttt ttgcgtccat gcagtagagc
     4441 gtcttgttgt cgctgccgaa aaacaccttg tcgccacaga ttgctggtga gccaaacacg
     4501 ttgtcaccaa cgggtgcatt ccagacttcc ttgccgtctg caatattcag gcaatagaag
     4561 gtgtctaagt ccgagcctgt aaagacaaac tggtcgtaaa cggctggtgc ggtcaccttg
     4621 tcgccgctct tgaatgtcca cagctttgcg ccagtgagtg catcgaagca gaccagattg
     4681 gcgtcaccaa cgattgccca gacttttccg gacgaggcaa caaccgggtt gactattccg
     4741 tcatcggtct tgctctccca gaccagctcg ctagtttcct ggttgaggca gtacagtttc
     4801 tggtcctgcg agccaaaata gaccttgcca tccagcaggg ccggtgcaga ggcgattccc
     4861 ctcccggcct tgaagctcca gtattcatag ccgctgtcgg cgttcaggca gtggaagttg
     4921 ccgcccatgt cgccaaagaa tgccactcca ctactaactg ctggtgaaga ctcgatcttt
     4981 gaggagaact ttgatgtcca gaccgggttt tttgacctgg tgttcacaca gtagaacttt
     5041 ccagcttcat cggcaaaata aatcttctcg ccagagatgc acggtgtgtt ccagattttt
     5101 gcgtctgcct ggaagctcca ttcttccttg cctgtcggga tgtccaggca ataaagcttg
     5161 tcactgccaa agtacgcttt tccgtttgat gcaaccggtt ggcccctcag gggttccttt
     5221 gattcaaact cccacgcctt ctcgattgcc ggctgggccg agattccacc tgttgcggtg
     5281 ccagtccttg acatgttccc catcatcatt ggccagtcct ggtgtgttgc cacttgcttg
     5341 ccagtgtctg tgccgccact atttgcccca tcgccgcttc cacagcctat gagtgggagg
     5401 gcgagaacca tgaataatac tgtcaaaaca gcgaatttct tcataatact cctttccatg
     5461 gcatttttga cacggaaaaa caggatatgt ttttttggac acttcagaat gctttatgga
     5521 acagcatttg tcattgcttt aagaatcttg gttttgcacg ggtccacttg aacttatcca
     5581 gcttggggga taaattaggg aggatgaaga cg
//
LOCUS       DAOSRI010000098         5607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13170_length_5607_cov_4.172911, whole genome shotgun sequence.
ACCESSION   DAOSRI010000098 DAOSRI010000000
VERSION     DAOSRI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13170_length_5607_cov_4.172911"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK717_03915"
     CDS             complement(<1..991)
                     /locus_tag="PK717_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR46841.1"
                     /translation="MDKNSAVQIILSSAEEIRSWSHGEVKKSETISYRNFKPEPDGLF
                     CEKIFGPVKDYECRCGKFKGARYKGVICDRCGVEVTINKVRRERFGHIELAAPVVHTW
                     YLKTMPYIALLLKVTGKVLEKITYFINYVVVDPGNAPNVQKMQLLTDEEYRELRRKYS
                     FKAKKGAEAILDLIGEIDLEKLNTELYEELGSGSAQRKAKAIKRLDVVQAFNETSRSP
                     INMILEVIPVIPPDLRPLLQLDGGRFATSDLNDLYQRVINRNTRLKKLMEVKAPDIMI
                     QNEKRMLQESVDALFDNSKRDRPILGTANRPLKSLSDILKGKEGRFRQNLLGKR"
     gene            complement(1001..3085)
                     /gene="fusA"
                     /locus_tag="PK717_03920"
     CDS             complement(1001..3085)
                     /gene="fusA"
                     /locus_tag="PK717_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR46842.1"
                     /translation="MKTFDTQSIRNVALCSHGGAGKTSWVEAAAFDTGLIERMGNVIA
                     GTTISDFDPDEAKRQISINLSVVPVVHQNTKINLLDTPGFFDFVGETLEGVRAAESAL
                     IFVDVGGGVQVGTEKVWQYCEDRKLPRIIVINKMDREHANFDNALAQIEETLTKNYLV
                     LTFPIGEGPQFKGVVDVIKGKSYTFADGGKKVVEGDIPADLKSKQEAYYNKLVEVVSE
                     SDDELLEAYLEGKIPTPEQVVKALHESVKNGKVYPVMATSSLSNIGTQNVLDAIIDYF
                     PSPDHAQPESAKDAKTEQEIKVKTDPSQPFSALVFKTTADPFVGRITYLKVITGSLTP
                     DTKLENINKDEEERISVVSIPIGKKQDNVSELKAGDIGVLTKLSKTLTGETLADPVKP
                     IIIKGIDFPKPVLRMSLKAKSKADDDKIGVAVPRLLEEDSTLEYTRNEETREIILGGL
                     GEAHLQAAAEKLARKFGVATTLSIPKIPYRETIKKAVKVQGRHKKQTGGHGQFGDCWV
                     EVKPLPRGSGFVFEESVFGGSIPKNFIPAIEKGFRESIQVGTLAGYPVIDISVNVYDG
                     SYHPVDSSEMAFKLAANLAYRKAFEDGGSVLLEPIYMLTITVPDQYMGDIMGDISSRR
                     GKIIGSEAHGKIQVIKATAPLIEVQRYAIDLGSITGGRGSFSMEFDHYEEAPPRVVEE
                     VVAKAKAEQAEQ"
     gene            complement(3098..>5607)
                     /locus_tag="PK717_03925"
     CDS             complement(3098..>5607)
                     /locus_tag="PK717_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46843.1"
                     /translation="QKGGEIVEVEPLDVDLIDISPNQVFSASATIIPFLEHDEANRAL
                     MGCNMQHQAVPLIQPEAPRVGTGMEHQIVVDNCSVVLAKESGVVSYVDAGTIKIASLD
                     AQPVKFKLGSINSEALYRFLGEDIANVGRIGDLVDGTVARKLIQNNFFKVRTISRDEV
                     ETINLGEFVKVERNDELIGKILVEPALDSEGKTLIAAGKKITETGLEKLYKAGIRDVI
                     VEVEGSPENLPVAILKVAASRNSFLGKILLRGPKEQEIFDNIDSSFVTEDVLRQLLQL
                     GATEIRVVDIMALHEEEVYTFNQEMLNSSRILAEDIYDSKEVVAYKGSYLTVDLLKRL
                     ASIGVDDIHVSYETTYKLQKFFRSNQDICINQRALVRKGDFVSAGDVIIDGEACDRGD
                     LALGQNVLVSYLPWRGYNYQDAIIVSERLVYDDVYTSIHIKEYKLAVRDTKVGPEELT
                     REIPNVSEEMLRNLDENGIVRVGTEVKSGDILVGKVTPKAESEFTPEMKLWRAMFDRK
                     GQDVRDSSLRVSPGEKGVVIGTQLFKRDKSDELQVGLNMLAKVYVAEKRKIQVGDKMS
                     GRHGNKGVISKILPIYDMPFLEDGSPVDIILSPLGVPSRMNIGQILECNLGYAARQLG
                     VYFATPIFSGATEKDVVDALEKASLPKDGKMNLYDGYTGEKFENMVTIGIAYMLKLNH
                     LVEDKIHARSTGPYALITQQPLGGKAQFGGQRLGEMEVWALEAYGAAHTLREMLTLKS
                     DDTEGRIKAYEAICRGKPIPESGTPESFKVIQRELRGLCIDLQIIGEKEDKSKSDKGL
                     FNMKVDAAIQESMDADQMKVIVPETFSISDEIELDEE"
BASE COUNT         1223 a         1311 c         1327 g         1746 t
ORIGIN      
        1 cacgttttcc aagcagattc tgcctgaatc ttccctcttt ccccttgagt atgtctgaca
       61 gagatttgag tggtctgttg gccgttccaa gtatcgggcg gtccctcttt gagttgtcaa
      121 acagggcatc aacggactcc tggagcatcc ttttttcgtt ctggatcatg atgtcagggg
      181 ccttgacttc catgagcttt ttgagcctgg tattacggtt gatgacgcgc tgatagagat
      241 cgttaaggtc agatgtggca aatcttccac catcaagctg gagcaatgga cggaggtctg
      301 gtgggataac cggaattact tccagtatca tatttattgg ggacctgctt gtttcattga
      361 atgcctggac aacatccagc ctcttgatgg cttttgcttt gcgttgtgct gagcctgagc
      421 caagctcttc atagagttca gtgttgagtt tttcgaggtc gatttcacca ataaggtcca
      481 ggattgcctc ggcgcctttt ttggccttga aagaatattt tcttctaagc tccctgtatt
      541 cttcgtcggt gagaagctgc atcttttgga catttggtgc atttcctggg tcaacaacaa
      601 cgtagttgat gaagtatgtt atcttttcaa gtaccttgcc agtgactttg agcaggagag
      661 cgatataagg catggttttt agataccatg tgtgcacgac cggggcggcc agttctatgt
      721 ggccaaagcg ctcacgtctt accttgttga ttgttacttc aacaccacat ctgtcgcaga
      781 ttacgccttt gtaccttgcg cccttgaatt taccgcaacg gcactcgtaa tccttgactg
      841 gtccgaatat cttttcgcaa aaaagcccat ctggctcggg cttaaagttg cgatatgaga
      901 tagtttcgga ttttttgact tcgccatggc tccatgaacg gatttcctcg gctgaagaca
      961 gtattatctg aacggcggag tttttgtcca cagttcctcc ttattgttcg gcctgctcgg
     1021 cttttgcctt tgcaacgacc tcctcaacga ctcttggcgg tgcttcttcg taatgatcaa
     1081 attccatact gaaagatccc ctacctcctg tgatggagcc aaggtcaatt gcgtacctct
     1141 gtacttcgat aagcggggcg gttgctttta tgacctggat ctttccatga gcctcgctac
     1201 cgattatttt tcctctcctg gagctgatgt cacccatgat gtcacccatg tactggtccg
     1261 gtacagttat tgtgagcata tagattggct caagaagaac ggacccgcca tcttcaaacg
     1321 cttttctgta tgcgaggttt gcggcaagtt tgaatgccat ttcagacgag tcaaccgggt
     1381 ggtatgaacc atcgtaaacg tttaccgaaa tatcgatgac aggataacct gccagtgttc
     1441 ctacctggat ggattccctg aacccctttt cgattgcagg gatgaagttc tttggaatgg
     1501 aaccaccaaa cacggattcc tcaaagacaa atcctgaacc ccttggaagt ggcttgactt
     1561 caacccagca gtcaccaaac tgaccatggc caccggtctg tttcttgtgg cgtccctgga
     1621 cttttactgc ctttttgatg gtctcgcggt atggaatctt tggaattgat agtgttgttg
     1681 caacgccgaa tttccttgct aacttttcgg ctgcggcctg caaatgggcc tctccaagtc
     1741 cgccaaggat gatttccctg gtttcttcgt tgcgtgtgta ttcaagggtg gaatcttctt
     1801 cgaggagtct tggaactgca acaccgattt tgtcatcatc agcttttgat tttgccttga
     1861 gagacattct aagaactggt tttggaaaat ctatgccttt tataatgatt ggtttgactg
     1921 gatcagcgag agtctcgccg gtaagggttt tgctgagttt tgtgaggacc ccaatgtcac
     1981 cagccttgag ctctgaaacg ttgtcctgct ttttgcctat cgggattgat acaacactga
     2041 tgcgctcttc ctcgtccttg ttgatgtttt caagttttgt atctggcgtg agtgaaccag
     2101 tgatgacctt gaggtatgtg attcttccaa caaatggatc agcagttgtc ttaaagacga
     2161 gtgctgagaa tggctgggat gggtcagttt ttacctttat ttcctgctct gtttttgcat
     2221 ctttggctga ctctggttgt gcgtggtccg gcgatgggaa gtagtcgatt attgcatcaa
     2281 gaacattttg tgttccaata ttggagagtg aggatgtggc catgactgga tagaccttgc
     2341 cattttttac agactcgtgg agtgctttga caacctgttc cggggttggg atttttcctt
     2401 caaggtatgc ctctagcagc tcatcatccg attcagaaac gacttctacg agcttgttgt
     2461 aataggcttc ctgttttgac ttgagatcgg ccgggatgtc gccttcgaca actttcttgc
     2521 caccatcggc aaatgtgtat gattttccct ttataacatc gacgacgccc ttgaattgtg
     2581 ggccttcgcc tattgggaaa gtgagtacga ggtaattttt ggtgagggtc tcttcaatct
     2641 gggcaagggc attgtcaaaa tttgcatgtt ccctgtccat cttgttgatt acgattattc
     2701 ttgggagttt tctgtcttcg caatactgcc agaccttttc agttccgacc tggacaccgc
     2761 caccgacatc aacaaagatg agtgcgcttt cagcagcgcg gacaccttcg agtgtttcac
     2821 cgacgaaatc gaagaaacct ggagtgtcaa gaaggtttat tttggtgttc tggtgaacaa
     2881 caggaacaac agacaggttt attgaaattt gcctttttgc ttcgtccgga tcaaaatccg
     2941 atattgttgt cccggcgatt acattgccca ttctttcaat aagccctgtg tcaaaagcag
     3001 cggcttctac ccatgaggtt ttaccggctc caccgtggga gcatagagcc acattcctga
     3061 ttgattgggt gtcaaaagtc ttcattggtc ctccagtcta ttcttcgtct agttctatct
     3121 catcgctgat actgaaggtt tctggaacga taaccttcat ctggtcagca tccattgatt
     3181 cctgtatggc tgcgtcaacc ttcatgttga agaggccctt gtcagatttt gatttgtctt
     3241 ccttctcacc aataatctga agatctatgc aaagtcccct gagttctctc tggatgacct
     3301 tgaatgattc cggtgttcct gattcaggga tcggtttccc cctgcagatt gcctcgtagg
     3361 ccttgattct tccctctgtg tcatcggatt ttagggtgag catttctctt agtgtgtgtg
     3421 ctgcaccgta ggcttcgagt gcccagactt ccatttctcc gagtctctgg cctccaaact
     3481 gtgcttttcc gcccaatggc tgttgggtta tgagggcata tggtccggtt gaacgggcgt
     3541 ggatcttgtc ttcaacaaga tggttgagtt taagcatgta tgctatgcca atggtaacca
     3601 tgttttcaaa cttttcgccg gtgtagccat cataaagatt catcttgcca tcttttggaa
     3661 ggcttgcctt ctcaagcgca tcaacgacat ctttttcagt tgcgccggag aaaattggcg
     3721 tggcaaagta aacgccaagc tgtctggctg cataaccaag gttgcactca agaatctggc
     3781 cgatattcat acgcgaagga acaccaagtg gtgaaagaat tatgtctact ggtgaaccat
     3841 cctcaaggaa aggcatgtca taaattggga ggattttcga gatgacaccc ttgtttccgt
     3901 gccttcctga catcttgtcg ccaacttgga ttttacgttt ttcggcgacg tataccttgg
     3961 caagcatgtt caatccaacc tgaagctcgt ccgatttgtc cctcttgaaa agctgtgttc
     4021 cgattacaac acccttttct cccgggctta ccctgagcga tgaatcacgg acgtcttgac
     4081 ccttgcggtc gaacatggcc ctccagagct tcatttctgg ggtaaattcg gattcggcct
     4141 ttggggtgac ctttccgacg agaatatcgc cggatttgac ctcggttccg acccttacga
     4201 ttccattttc atcaagattg cgaagcatct cttcggagac gtttggtatt tccctggtca
     4261 gttcttccgg tccgaccttg gtgtctctta ctgcaagctt gtattctttt atatggattg
     4321 aagtgtaaac atcgtcataa acaaggcgct ctgaaacaat gatggcgtcc tgatagttgt
     4381 accctctcca tgggagatat gaaacaagaa cgttctggcc aagtgccagg tcgcccctgt
     4441 cgcaggcttc tccatcgata atgacatcgc cagccgagac gaagtctccc ttacggacga
     4501 gcgccctctg gttgatgcaa atgtcctggt ttgacctgaa gaacttttga agcttgtagg
     4561 ttgtttcata ggatacatgt atatcatcaa cacctattga ggctaggcgc ttcaaaagat
     4621 caactgtaag gtacgaacct ttgtatgcta caacttcttt ggaatcatag atgtcttcgg
     4681 caagaattct tgaagagttg agcatctcct gattgaaggt gtaaacttct tcctcgtgaa
     4741 gggccattat gtcgacaacc ctgatttcag ttgcgccgag ttgcagtagc tggcggagca
     4801 catcttcggt tacaaaggaa gaatcgatat tgtcaaatat ttcctgttcc tttggccccc
     4861 tgaggaggat ttttccaagg aaactgtttc ttgaggctgc aactttgaga atggctacag
     4921 gaaggttttc gggtgaaccc tcaacctcga cgattacatc tcttatgcct gctttgtaga
     4981 gtttctcaag gcctgtttca gttatctttt tgcctgcggc gatgagggtt tttccttcag
     5041 aatcaagggc tggctcgaca aggattttgc ctatcagttc gtcatttctt tcgactttta
     5101 cgaactcgcc taggttgatt gtttctactt catccctgct tatggttctt actttaaaga
     5161 aattgttctg gatgagcttt ctggccactg ttccatcaac gagatcgcct atcctgccga
     5221 cgtttgctat atcttcacca agaaacctgt agagtgcttc agagttgatg ctaccaagct
     5281 tgaatttgac tggttgtgca tcaagtgatg caatttttat tgttccggca tcaacatagg
     5341 agactacgcc agactctttg gccaggacaa cagagcagtt gtccacgacg atctggtgct
     5401 ccatgccagt gccaactctt ggggcctctg gctggatgag tggaacggcc tggtgttgca
     5461 tgttgcagcc catgagggca cggtttgctt catcgtgctc aaggaatgga atgattgtgg
     5521 ccgaagctga gaagacctgg tttggagaaa tgtctataag atcaacatcg agaggctcaa
     5581 cttctacaat ttctccaccc ttttgcc
//
LOCUS       DAOSRI010000099         5563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13328_length_5563_cov_3.733115, whole genome shotgun sequence.
ACCESSION   DAOSRI010000099 DAOSRI010000000
VERSION     DAOSRI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13328_length_5563_cov_3.733115"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK717_03930"
     CDS             complement(<1..710)
                     /locus_tag="PK717_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46844.1"
                     /translation="MKLLLEENSLWKKRARAPRIIRFHVARNNSSVAVIEANIDGAAQ
                     TYLLNMKTGDLDVIQSLDKDYYSVLSPDGHFLHFHKDTKGNQHGHRCRMPTSGGEIED
                     LTPELPDYYAYRIEFSDDSKRCIFPAYFDDETNLYALFFNNKGGLVDRKILFKTKGDL
                     GWVKMSRDGNMVAVSSSRSNRNDETKLLVLSVDTGDEYKQLKLDCETSAYAHLFVPCS
                     SKLIISTEDGDHKKFYLW"
     gene            888..1487
                     /locus_tag="PK717_03935"
     CDS             888..1487
                     /locus_tag="PK717_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR46845.1"
                     /translation="MQNHLVGIDADYVEYPHAVTAAANCVSDITKWVFGECGNNHYDF
                     VVGHSMGGIIALELAAKFNLAFRQIILVDCSLRPANMFYRNLMTEGHMEQYGSKIMQM
                     MQEEAPYYQDDLKQSLKEGFDYTQYLKVLNQNVYAIYGDRVQKNYPDRINDLCLDEET
                     LGRIKLLFVENACHMPMIENPSALAFMILDIVYGRLVLE"
     gene            1498..2178
                     /locus_tag="PK717_03940"
     CDS             1498..2178
                     /locus_tag="PK717_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46846.1"
                     /translation="MRISFEENEKDVIFLIGDFDEKYVPVFKSCFWQEENGSYFKSFP
                     KDSKYIDNAKENFRLHSKEMFDQLGYFSECKWEEALYDFALRMKENMIDWWLTGSCAA
                     CIRGIALKPHDVDAMFGSKDLDRITDVFPDKIIHPILNTNGWVTRNFGVLFWHARIDL
                     ASDPVAELDKPEPIDCGPYARRHLETIRWRGLDIKVPPIELTLAANKRRKRFERAKLI
                     EDFLRSKE"
     gene            2238..3203
                     /locus_tag="PK717_03945"
     CDS             2238..3203
                     /locus_tag="PK717_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46847.1"
                     /translation="MEENILREKLSRRGFDENRIEQALETIASFEKYLKAAALDPSDK
                     KPLVDYCSKLIRQNKNNWYNIFALAVYGKSIGCENLTINALELLDGSEALWNLRDKLD
                     EAFGEIVASKYYEGVRFPELGEPNEIRPEAMGAVIGRVGSDLGQEKTASLIGGCLRNI
                     KDSWYEEDVKLFEECGKDIDKFIKTRGDRFLSELETLKNEGNLYFTQPITDEVIEFVK
                     SNPEIHYGVRAGNIFYDTKIPHQAAKMLSESDPVKRRYHYCHCPWVKESLANGKSPVP
                     PVFCNCSAGFHKRMWEVVLGKPVKCEVLESVLKGDDRCRFKIDLG"
     gene            3401..3775
                     /locus_tag="PK717_03950"
                     /pseudo
     CDS             3401..3775
                     /locus_tag="PK717_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            4880..5341
                     /locus_tag="PK717_03955"
     CDS             4880..5341
                     /locus_tag="PK717_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOR46848.1"
                     /translation="MEKKSIKALVIFDTTFGNTKTIAEAIAKELGSGAKAVQASSVGA
                     SELKGLDLLVVGSPIIGWKPSEKMEAFLNGLAKEQLKGTKAVAFDTRVKIFIHGDAAR
                     KISERLEVAGAEIVSKPQMFYVKGREGPLFDGEVEKSVEWAKSIKSLLKTK"
     gene            5352..>5563
                     /locus_tag="PK717_03960"
     CDS             5352..>5563
                     /locus_tag="PK717_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOR46849.1"
                     /translation="MNRETAILAKKTLLYFALFLVIMGLAIFLPAWSLAYWEGWAYIS
                     IYFVLVLLVTLYLLRNDPELLKRRLR"
BASE COUNT         1563 a         1162 c         1410 g         1428 t
ORIGIN      
        1 tcccataaat aaaatttctt atgatctcca tcttctgttg aaataatcaa cttgctgctg
       61 caaggaacga acaagtgggc gtatgcagat gtttcgcaat caagcttgag ttgcttatat
      121 tcatctccgg tgtccacact taatacaagc aactttgttt catcatttct attgcttctg
      181 gatgaagaca ctgctaccat atttccatct cttgacattt ttacccagcc aagatcaccc
      241 tttgttttga acaagatctt cctatcaaca agcccccctt tgttattaaa aaataatgca
      301 taaaggttgg tttcatcatc aaaataagca ggaaatatac atcttttcga atcatcagag
      361 aactcaattc tataagcata ataatccggc aattcagggg ttagatcctc aatctcacca
      421 ccacttgttg gcattctgca tctatggcca tgctggttac ctttagtgtc tttatggaaa
      481 tgcaggaagt gaccatcagg gcttaaaaca ctataataat ctttatctag actttgtatt
      541 acatctagat cgccagtttt catgtttaat aaatatgttt gcgctgcacc atctatattg
      601 gcctcgatta cagcaacaga actgttgttc ctggccacat gaaatctgat tattctgggt
      661 gctcttgcac gttttttcca taatgaattt tcttcaagaa gtagtttcat gcacctccta
      721 caacatgtat aatactgaga tcaaaagcaa tagctaccga attgttgcag atgcttgttg
      781 ctatggcgat ttcatttatc atgtagtgtt ggaatttgga aaggcaacga aaaatgaagg
      841 gtttgttttt gcacggcgta aactgtacgt ccaggatctg gtcgcagatg cagaatcacc
      901 ttgttggcat tgacgctgac tatgtggaat acccccatgc ggtcactgcc gctgcaaact
      961 gtgtttcgga cattacaaag tgggtttttg gtgagtgtgg gaacaatcat tatgattttg
     1021 tggttggcca cagcatgggc ggcattatcg cactggagct tgcggcaaaa ttcaacttgg
     1081 catttcgcca gataatactg gttgattgca gtctcaggcc tgcaaacatg ttttatcgca
     1141 atttgatgac agaagggcat atggaacaat atggcagcaa gattatgcag atgatgcagg
     1201 aagaggcgcc atattatcaa gacgacctga aacagtcact taaggaggga tttgactata
     1261 cgcaatacct caaggtgttg aatcagaatg tctatgcgat ctatggagac cgggtacaga
     1321 agaactatcc agatagaatc aatgatttgt gccttgatga ggaaacattg ggaaggatca
     1381 aattgttgtt tgttgaaaat gcctgccata tgccaatgat agagaacccg tcagcgctgg
     1441 cattcatgat tttggatatt gtctatggca ggctggtttt ggaatagagg agataaaatg
     1501 aggatcagtt ttgaagaaaa cgaaaaagat gtgatatttc tgataggtga ttttgatgag
     1561 aagtatgttc cagttttcaa atcatgcttc tggcaggagg aaaatggctc atatttcaaa
     1621 agctttccaa aggactcaaa atacattgac aatgccaagg aaaatttcag actccactca
     1681 aaagagatgt ttgaccagct tggctatttt tcagaatgca aatgggaaga ggcgttgtat
     1741 gattttgcgc tcagaatgaa agaaaacatg attgactggt ggctcacagg gagctgtgcc
     1801 gcttgcataa gggggattgc actaaaaccg catgatgttg atgccatgtt tggttcaaaa
     1861 gatttggacc gtattactga tgtgtttcca gacaagatca ttcatcccat tctcaacaca
     1921 aacggctggg tgaccaggaa ttttggtgtt ctgttctggc acgcaagaat tgatcttgca
     1981 tctgaccctg tggccgaact ggacaaaccg gagccgattg attgtggccc atacgcaagg
     2041 agacatctgg aaaccataag atggcgtggc ctggacatca aagtgccacc aattgagctg
     2101 accctggccg ccaacaagag aagaaagaga tttgaaagag caaaactgat cgaggatttt
     2161 ttgagatcca aagagtgatt tccattcagc ttgacatcac ttgctgacaa ctgtaagttg
     2221 ttgtcaaagg agccataatg gaagaaaaca ttttgagaga aaagctttcc aggaggggtt
     2281 ttgatgaaaa caggattgag caggcgcttg aaacaatcgc cagttttgag aagtatctca
     2341 aagcagcagc tttagaccct tcggacaaaa agccccttgt tgattattgt tcgaagctga
     2401 tcaggcaaaa taaaaacaac tggtacaata tctttgccct tgcagtctac ggcaagtcga
     2461 tcgggtgcga aaacctgact ataaatgcgc ttgagttgtt ggatggctcg gaggccctgt
     2521 ggaacctgag ggacaaactt gacgaggctt ttggtgagat cgtggcttca aaatactacg
     2581 agggtgtcag gttccctgag cttggcgagc caaacgagat caggcctgaa gcaatggggg
     2641 ctgtcattgg cagggtggga tcggacctcg gccaggaaaa aacagcttcc ctgattggcg
     2701 ggtgccttag aaatattaaa gattcgtggt atgaggaaga tgtgaagctt tttgaggagt
     2761 gcgggaaaga tattgacaaa ttcatcaaga caagaggaga tcggtttctt tccgagctcg
     2821 aaacgctcaa aaatgagggg aatctctatt ttacccagcc aatcaccgat gaggtcatcg
     2881 aatttgtgaa atcaaaccct gaaatccatt atggtgtgag ggccggaaat attttttatg
     2941 acacaaaaat tccgcaccag gccgcaaaaa tgctttcaga aagtgacccg gtcaaaagac
     3001 gctaccacta ttgccactgc ccgtgggtca aggagtcgct tgccaatggg aaatcgcctg
     3061 ttccgccggt gttttgcaat tgcagtgcgg ggttccacaa gaggatgtgg gaagttgtcc
     3121 ttggaaaacc agtcaaatgc gaagttcttg aatcagtcct gaagggtgac gacaggtgca
     3181 ggttcaaaat tgatctgggc tgattcgctt ttttctggcg ttcatttttg attgaagcaa
     3241 aattgcatag ggcctgctgc ttttttctgt tgtctttgaa aaagtctcca aagactgttt
     3301 gtctttgttg ccacaaaaaa ggaaaaatca aaaaatatct ggttctggag atgttgcgca
     3361 ccgtgctcaa aagggctaat aattggagtg aggcaaaaag atgcaaaaca gcgagaagcc
     3421 aaaaagcatt gatgaataca tctccagctt tccggaggag acccgccttc tacttgagaa
     3481 aatgcgggaa gtgataaaga atgccgcacc tggggccaaa gaagtgataa gctaccagat
     3541 gccggcattc aagctgaaca aggtcctggt ctattttgcg gcccacaaaa accatatcgg
     3601 tttctatccg acatcctcgc cgatcattgt tttcaaaaaa gagctctcat cctacaaaca
     3661 atcaaagggg gccgtccagt tcccctttga caggccgata ccatttgaca tcgtgaagaa
     3721 aattgtggaa ttcagagtag aagaggacag gcaggaggct gaaagcaaga agaagtctgt
     3781 gcccaagacg caaaaaagaa acgctccagc tcttgctttt gccattaaga caccatgcaa
     3841 atttcctgtt gaaaaagagt acacccgttg catcgaaacc ttggcaaaat ccggtgtcat
     3901 tgggcctctc cctaaatcgg gaagtattgg tgcgattggg attgacggga aagagtaccc
     3961 ggtgccaacg cttgggcagg ttctggggct tttctctggc aacagagagc tggtggaaaa
     4021 gaaatttccc cagggttttg accgactgga gcttgttcca atggcggccc cggtgctttt
     4081 tcttgccgac ttgatgaggg cgacaatcct caggcgcatg gcggaaggaa aaattttccg
     4141 caccaggcga ggcccttttg atcctctcgt gccagtccgc gtcaacaagg aaaaacacgt
     4201 ctggatttgg gagaccctga aagaagtcct ggacactgac gggcttgtct attttccgga
     4261 ggagtacttg gtcagccacc gcgggcagac caaactggaa gtgattgcca acaggcggat
     4321 ttgtgctgtt cctggctggt ccgttgggct ctcggagagc ttgcccatca tgcccggcca
     4381 gggtgaaggc aagattgttg ccggaagaaa acagcttgag ataggatttt caccgaatga
     4441 atacaggagc atcttgcagg gccgcgccta tcagggcgaa actggaaaca cactcgagga
     4501 ctttattgtg aggttcatct cccgccttga ggagaccggc gaggtaagca acgacatcat
     4561 tgatgaaaat gccctttggt gcctcgcaca gtatgtaaag atcccgtatg cagactgcgt
     4621 gccggttggg cgctggcacc gcaccctcgg gcgcgcccgc ctggacatgc accggtcaaa
     4681 caacaagctg tgcacaaaaa actgtggcgg cgccaccgtg gtgaggctga ctgggagttc
     4741 tgattcgtaa gggttttggg aagtagcacc accaaggccc ggaaacaaaa aatcgcacaa
     4801 tggtttcaag ggtttgcatt aagtagcgat ttgactaaat atatgggtaa acaggcattg
     4861 cgaaaggcag ggtgaccgga tggagaagaa atcaatcaag gccttggtta tttttgacac
     4921 aacttttggg aacacgaaaa caatcgcaga ggcgattgca aaggagctcg ggagcggggc
     4981 aaaggcggtg caggcctcca gcgttggcgc ttccgaattg aaggggctgg atttgcttgt
     5041 tgtcggcagt ccgatcattg gctggaaacc ttctgagaag atggaggcgt ttctcaatgg
     5101 cctggccaag gaacagctaa agggcaccaa agcagtggct tttgacacga gagtgaaaat
     5161 attcatccac ggggacgcgg ccagaaagat ctcggaaagg ctcgaggtgg ccggtgctga
     5221 aattgtttcc aagccgcaga tgttttatgt caaaggcaga gagggcccgc tttttgatgg
     5281 tgaggtggaa aagtctgtcg agtgggcaaa atctataaaa agcctgctaa aaacaaagta
     5341 gttttggccc catgaacaga gaaactgcca ttctggccaa aaaaacactg ctgtactttg
     5401 ccctgttcct cgtgatcatg ggactggcaa tctttttgcc ggcgtggtcg cttgcttact
     5461 gggaaggctg ggcctatatt tccatctatt ttgtccttgt cctgcttgtc acgctctacc
     5521 tgctgagaaa tgacccggaa ctgctaaaac gaaggctccg cag
//
LOCUS       DAOSRI010000100         5542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13386_length_5542_cov_4.377802, whole genome shotgun sequence.
ACCESSION   DAOSRI010000100 DAOSRI010000000
VERSION     DAOSRI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13386_length_5542_cov_4.377802"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK717_03965"
     CDS             complement(<1..327)
                     /locus_tag="PK717_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46850.1"
                     /translation="MKKMAFLVLAAIMLTSCGQGVPTDKLYSNGTKATYQIFIDDINV
                     GKMIYTFKEGVYEGQKSLTIESDANYTYKDPRQPQDFEIKSKTTSIVNANSWKPLYSY
                     FKMEWKG"
     gene            415..2487
                     /locus_tag="PK717_03970"
     CDS             415..2487
                     /locus_tag="PK717_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46851.1"
                     /translation="MRRLSAFLVVLAISQSFCFAGGISSAPFPFKKTCIVELATSVNC
                     SYCPVAEKALKELESENDLSDMVIFAHHYKDSLSCYGGNSRMAEYGVNATPIAIFNGQ
                     DYYVGGDPNCKDRYRQKISDQLSQTSPFLVHLMGKIEKSKLSLTAWVVAWEYLPPDVN
                     YTFLVGRLDTKTDGRHYPWVTRDVVPKPTGTPIKFSNFSVTRFEATYDLLSIPEDEVF
                     ASFVLESFAKKNIFQVASWRPGCLQASSFEPSLGLKLDTSPSSVTISFPTGKKLEKIG
                     KVTFVDSKGKNYQVVPKLDGEKAIFTPSGKLPDGTYLLYIASGKDGLSSGSSILNSPA
                     FLFFKVEEKTTVPDPPEPPPTKPAKLLVEKLGFDLGEIPRKSGRELYFDIKNEGDEKL
                     SGKVAVDCEWLSVEPSTFDNAPATIKCTVDTEKMRPGVDESCIISITSNGGDATIGVK
                     ARTHMLPPLLNVEPAELNFGENLDKIITFEVKNLGESVISGTAKSAQSWLSITPEEFG
                     GDTNINVSINKAEIPKFEEPGKHTIEGTIVVETNGGLIEIKVKAIVVVEKQPIVIELM
                     VGTPFAVVDGKTKLLQAPPMIVSGRTLVPLRFVAETFGGQVLWDQATKTTTLKFESKK
                     VEISLRSGEAKATIKDETGVRTVELVPGLTIKSGTSFVPLRFFSEVLGATVEWFAQEK
                     RIRIVYLP"
     gene            complement(2484..3203)
                     /gene="minC"
                     /locus_tag="PK717_03975"
     CDS             complement(2484..3203)
                     /gene="minC"
                     /locus_tag="PK717_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOR46852.1"
                     /translation="MCGGADVTCFGQRVDVEYMATMPRKAIPDDAVTIKGTIDGVNVI
                     VDEKYDLVRLFSKLEQKIKSAKSFFSGNQIMVSLKERAFTKDEFDYFRSSLKLKYGVD
                     VAPLIPVQGEEEKPKIEEDKLFKTGPQLCNTKVISHTLRAGQSVEYEGDIVVAGDVNP
                     GAEVVATGNVFVMGAVRGRVCAGCKGNRESYIIAMDFEPVQLRIADKVAVSPSAQGLK
                     RELRTQKAFIEGDSIVVVSVR"
     gene            complement(3155..3745)
                     /locus_tag="PK717_03980"
     CDS             complement(3155..3745)
                     /locus_tag="PK717_03980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOR46853.1"
                     /translation="MTQENLTEARIILASGSPRRIELLKLLGVPFVAINADVEEKNFS
                     SAEFTIKHNSQKKALAVFANHPDKIVIACDTVVCIGEKVYGKPADKKQAKEYLRELSG
                     KTHSVLSCVTIIGQEFSPLSKTQESKVTFKNFGEETIESYIRDVNTLDKAGAYGIQEK
                     GWLLVSGIDGPLDNVMGLPLSCVVELMSLASDRGLM"
     gene            complement(3717..4757)
                     /gene="amrS"
                     /locus_tag="PK717_03985"
     CDS             complement(3717..4757)
                     /gene="amrS"
                     /locus_tag="PK717_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOR46854.1"
                     /translation="MKLCELWLEDKDNSVVCTACIRRCRIAIGRTGACATRKNEGGKL
                     YSLTYGHISSIALDPIEKKPLFHFFPGSHVYSIGGWGCNFGCGMCQNYEIAHIVPGLR
                     DEDAVSPEKLISDALAKNADGMAFTYNEPAIWPEFVRDVFKLAKKHDLYTVLVTNGSS
                     TKESIDYYGPYCDAYRVDIKGMTDITLGRIGIKGVDPSEILEGTVWARDRWGMHVECV
                     TNIIPTVNDSESELRAIATWIRDNLGPKVPWHVTRFYPALRFRHLMPTNLPSLEMAEK
                     IGKQVGLSYVYIGNVHTQRGENTYCPRCQALIIEREGYKIKQDLSAMGRCTRCGELLG
                     IVTSDTRKSYRS"
     gene            complement(4764..>5542)
                     /locus_tag="PK717_03990"
     CDS             complement(4764..>5542)
                     /locus_tag="PK717_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR46855.1"
                     /translation="IKMDVLIGKSTTIKELFDGGYKAVFIGSGAGLPSFLNIPGENSN
                     GVFSANEYLTRVNLMKAYKFPNFDTPVWLGKRVAVVGCGNVAMDSVRTALRLGAEKAM
                     IVYRRTSNEMTARVEEYHHAQEEGVEFNWLTNPLEILADEKGKVTGMKCVKMRLCEPD
                     ASGRCRPEPIPGSEFTVELDTVIIALGTSPNPLIAQTTPELKTAKHGNLIVDDKYQTT
                     MPGVFGGGDAVTGSATVIQAMGAGKIAARSIDEYIKANVK"
BASE COUNT         1336 a         1310 c         1266 g         1630 t
ORIGIN      
        1 gcctttccat tccatcttaa agtaggaata aagtggcttc catgagttgg cattaactat
       61 tgaggtagtt ttcgatttta tttcgaaatc ctggggttgg cgtggatctt tataggtata
      121 attggcatca ctctctattg taaggctttt ttgcccttca tagacgcctt ctttgaacgt
      181 gtaaatcatt tttccaacat ttatatcatc tataaaaatc tggtatgtgg ccttggttcc
      241 attgctgtaa agcttgtcgg ttgggacacc ttgcccgcat gaagtcagca tgattgctgc
      301 cagtaccagg aatgccattt ttttcatatc aatcgaacct cctgttaaag gttatcattg
      361 caccctacct ggtcaaggct tgaaaggttc ccgaaacaaa tggataattc atccatgaga
      421 cgtttgtctg ctttccttgt ggtgttggca atttcacaat ctttttgttt tgctggcggt
      481 atttcatcgg caccatttcc gttcaaaaaa acctgtattg tagaactggc aacgtctgta
      541 aactgcagct attgccctgt tgcggagaag gcgctcaagg agctggaatc tgaaaatgat
      601 ctatccgata tggtgatttt tgcccaccac tacaaagatt cgctttcgtg ctatggtggc
      661 aattcgagaa tggcagagta tggggtgaat gccactccaa tagcgatctt taatggccag
      721 gactactatg ttggtggtga tcccaactgc aaagaccgct acaggcagaa aatatccgac
      781 cagctttcgc aaaccagccc tttccttgtg catttaatgg gcaagataga gaaaagcaaa
      841 cttagtctga ccgcctgggt tgttgcctgg gaatacctcc ctccggatgt caattacaca
      901 tttctggttg gccgtctaga cacaaaaact gatggaaggc attatccttg ggtaaccaga
      961 gatgtggtgc caaaacctac tgggactccg ataaaattct caaacttttc ggtgaccaga
     1021 tttgaagcta cttacgacct gctttccata ccagaagatg aggtctttgc atcttttgtg
     1081 cttgaatctt ttgctaaaaa aaatattttt caggtggcat catggcgtcc cgggtgcctg
     1141 caagcaagct catttgagcc atcgcttggc ctgaagcttg atacttcccc atcttctgtg
     1201 acgatatctt tccccactgg caaaaagctt gaaaaaatcg ggaaagttac cttcgtggat
     1261 tcaaagggca agaactacca agttgtgccg aagcttgatg gtgaaaaagc gatttttaca
     1321 ccatcgggca aattgcctga tggaacatat cttctttata tcgcgtctgg gaaagatggc
     1381 ctttcctccg gcagttcaat cctcaattcg ccagcattcc ttttcttcaa agtggaagaa
     1441 aaaacgactg ttccagaccc tcctgaacct ccgccaacca aacctgcaaa gctcctggtc
     1501 gagaaacttg gttttgatct tggcgagata ccgcgcaagt caggacgtga gctgtatttt
     1561 gacatcaaga acgagggtga tgaaaagctt tctggcaagg ttgccgttga ttgtgagtgg
     1621 ctctctgttg aaccatcgac ctttgataat gcgcctgcca caataaaatg cacggtggat
     1681 acggaaaaaa tgagaccagg ggttgatgaa tcatgcatta tttcaataac ctcgaacggt
     1741 ggtgatgcaa caattggggt caaagctaga acacacatgc ttccgccact tcttaacgtt
     1801 gaaccagcag agctgaattt tggggagaat ctcgacaaga tcataacttt cgaagtcaaa
     1861 aaccttggcg agtcagttat ctctgggact gccaaaagcg cccaatcatg gctgtcaatt
     1921 acgcctgagg aattcggtgg agacaccaat atcaatgtat cgatcaacaa agccgaaata
     1981 cccaaatttg aagaaccagg caaacataca attgaaggta cgatagttgt tgagacaaac
     2041 ggcgggctca ttgaaatcaa agtgaaagcg attgttgttg tcgagaaaca gccaattgtg
     2101 attgaattga tggttgggac cccgtttgca gttgttgatg gtaaaaccaa gctcctccaa
     2161 gctcctccaa tgattgtatc cggaagaacc cttgttccat tgaggtttgt tgcagaaact
     2221 tttggtggtc aggtcctgtg ggatcaggcc acaaaaacaa ccacgctgaa atttgaatcg
     2281 aaaaaggttg agatttcttt gcgttctggc gaggcaaagg ccacgataaa agatgaaact
     2341 ggagtcagaa cagttgaact tgttccaggt ctgacaataa aaagtggaac cagttttgtc
     2401 ccattgagat ttttctcgga agttctgggt gcgactgtcg agtggtttgc ccaggagaag
     2461 agaataagga tagtttattt gccctagcgg acgcttacta caactatcga atcaccttca
     2521 atgaaggctt tttgagttct gagctccctc tttaggccct gggccgatgg tgaaaccgcc
     2581 actttgtctg caattcttag ttggaccggc tcaaaatcca ttgcaatgat gtaggattct
     2641 ctatttcctt tgcagccggc acaaaccctt cccctgactg cgcccatgac gaaaacattg
     2701 cctgttgcaa caacctcggc ccctgggttc acgtctccag caacaactat atcaccttca
     2761 tattcgactg actgcccagc cctgagagtg tgggaaatga ctttggtatt gcagagctgc
     2821 gggcctgtct tgaagagttt atcttcctca atttttggtt tttcttcttc gccctgtaca
     2881 ggaatgagcg gagcaacgtc gacgccatac tttagtttta gcgatgacct gaagtagtcg
     2941 aattcgtctt ttgtgaatgc cctttccttt agcgaaacca tgatctggtt tccagagaaa
     3001 aaactttttg ctgacttgat tttttgttcc agttttgaga acagcctcac caagtcatat
     3061 ttttcatcga cgatcacgtt cacgccgtct attgttccct tgattgtgac ggcatcatcc
     3121 ggaatggctt ttcttggcat ggtggccatg tattctacat caaccctctg tccgaagcaa
     3181 gtgacatcag ctccaccaca catgatagcg gaagtcccat gacattgtcc agtggcccgt
     3241 caattccgct gacgagaagc catcccttct cctggatgcc ataagcaccg gctttgtcca
     3301 gcgtgttgac gtctctgatg tatgattcga ttgtttcctc gccgaaattc ttgaatgtta
     3361 cctttgattc ctgggttttt gagagcggag aaaactcttg ccctatgatt gttacacaag
     3421 acaacactga gtgtgttttt ccggaaagtt cccggagata ttcctttgcc tgtttcttgt
     3481 ctgcgggttt cccataaacc ttttcgccaa tacataccac cgtgtcgcaa gcgatgacaa
     3541 ttttgtcagg gtgatttgca aatacagcca aggccttctt ttgagagttg tgtttgatcg
     3601 taaattccgc gctggagaaa ttcttttctt caacgtcggc atttattgca acaaaaggaa
     3661 caccaagcag ttttaatagt tctattcttc ttggtgaccc tgatgctaga ataatcctag
     3721 cttctgtaag attttcttgt gtcacttgtg actattccta acagctcccc acaccttgtg
     3781 catcttccca ttgcggagag gtcctgtttt attttgtatc cctcgcgctc tattatcagg
     3841 gcctggcacc ttgggcaata cgtattctca cccctctggg tgtggacatt gccaatgtaa
     3901 acataggaaa ggccaacttg tttgcctatt ttttcagcca tctccagtga aggcaagttt
     3961 gttggcatga ggtgtctgaa acggagcgct ggataaaacc ttgttacgtg ccagggtacc
     4021 ttcgggccga ggttgtctct tatccaggta gctatggctc tcagctcgct ttcactgtca
     4081 ttgacagttg ggatgatgtt ggtcacgcat tcaacgtgca tgccccacct atcccgtgcc
     4141 catacggtgc cttcaaggat ttctgatggg tcaacaccct tgatgcctat gcgtcccagt
     4201 gttatgtcgg tcattccctt gatgtccact ctgtaggcat cgcaatatgg cccgtaatag
     4261 tcaattgatt ccttggtcga tgatccgttt gtgacaagga ctgtatagag atcgtgcttt
     4321 tttgccaact tgaaaacgtc cctcacaaac tctggccaaa tggctggctc attgtatgtg
     4381 aacgccatcc cgtcggcgtt ttttgccaaa gcgtcgctga tcagtttttc gggtgatact
     4441 gcgtcttcgt ccctaaggcc tgggacaatg tgggctattt cataattctg gcacattccg
     4501 cagccgaaat tacaccccca cccaccgatt gagtaaacgt gtgatccagg gaaaaaatga
     4561 aaaaggggct ttttttcgat agggtcgagc gcaattgagg agatgtgtcc ataagtcaga
     4621 gagtagagtt tgccaccttc atttttcctt gttgcgcagg cgcctgtcct gccaattgcg
     4681 attctgcacc gccttatgca ggcggtgcag accacagaat tatctttgtc ttcaagccaa
     4741 agctcgcaga gcttcatttt ctcctatttg acgtttgctt ttatatattc gtcgattgaa
     4801 cgtgcggcga tttttccagc gcccattgcc tgtatgacgg ttgcagaccc tgttactgca
     4861 tctccgccac cgaacacacc tggcatggtc gtctggtact tgtcgtcaac gataagattg
     4921 ccatgcttgg cagttttcag ttccggggtt gtttgggcta tgagcgggtt tggcgaagtt
     4981 ccgagtgcaa ttatgactgt atcgagttcc acggtgaact cggagcctgg tatcggctcc
     5041 gggcggcacc ttccagaagc atccggctca cagagcctca ttttgacaca tttcatgcct
     5101 gtgactttgc ctttttcgtc agcaaggatt tcgagtgggt ttgtgagcca gttgaattct
     5161 accccttctt cctgggcatg gtggtattct tcaaccctgg ctgtcatttc gttggatgtc
     5221 ctgcggtata caatcattgc tttttcagca ccaagcctca gggctgtcct taccgagtcc
     5281 attgcaacgt ttccacaacc tacgacagca acccttttgc ccagccacac tggggtgtca
     5341 aaatttggga atttgtaggc tttcataagg ttgaccctag tcaggtactc attggcggaa
     5401 aataccccgt tagaattctc gccagggatg ttcaggaagg atgggagtcc tgcccccgac
     5461 cctatgaata ctgccttgta gcctccatca aacagctctt ttatggtggt agatttgccg
     5521 atcaggacat ccatttttat ct
//
LOCUS       DAOSRI010000101         5515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13482_length_5515_cov_3.773260, whole genome shotgun sequence.
ACCESSION   DAOSRI010000101 DAOSRI010000000
VERSION     DAOSRI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13482_length_5515_cov_3.773260"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..937
                     /locus_tag="PK717_03995"
     CDS             35..937
                     /locus_tag="PK717_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46856.1"
                     /translation="MGDPPMAHWAASVIDQGEFPSPEAKIVADPEPSSQTKAVTFFEI
                     FDNNYKHKYWSAVMTNVYTKTIVVAIDDDMKIRYVRPVSTSKSPDPTILQYGQIIFDP
                     YNGATPDQLAFLNARIPQNDKFTPYAIAVRSAVASAVKLFVIDKEGYPAYQKKYPYGI
                     LSFRPGTLFVLPKMKTDSGKEISGAYFKGRPYVVIFVSSCASCRKRAREIANILGKFG
                     IKPDQVIFLSSSEKAKLEEFPGQVKDSHLFIDLKKELASLMLFDSTPSLLAVDSSSKV
                     VGQLDSIRLNGDAELNELLPKLTR"
     gene            1028..3562
                     /locus_tag="PK717_04000"
     CDS             1028..3562
                     /locus_tag="PK717_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46857.1"
                     /translation="MGRFTKVLVFLLSVHILFGQTVFVASGVEDEGPKPWTQYASDHM
                     HSSNALGRWDFNAYDKESWKLGLKGVESRGRNPVVDQGRLFIGDSDKIYCIDVFTGNI
                     LWSFEVSSPVAGSCAILGDTVIAPMNNRLLGLSTANGEVRWYNGDVGDNISSPTVYKD
                     DHGGKWMYFTTHDGKTGRAYKYSLNTGKLAWRIKTEWPCPGAMGVNSDYGYVGCAAHY
                     KASVLDDGSGGVVDGVEVGTQAFSATMAFDKYCICTMSGGEIIALPRPYSKKDPKIFD
                     TGGMTFAPPCSFGDTLIIGDDEGRLVRFNTEGKIIWDKKMPGPVTDGCTVMGDHVLVP
                     VGSKGSDTAGVYIIDGETGEQVKKIPLKSSYVFQPVVAWDRMFVEHGESNNYRNRTLS
                     CFGKPPRTSDQEPKIRVDGGKINAEVGYKGETSRQITITNDGKIPLELVLLGDSLLFP
                     SKEVLKLAPKEKDTIRVAIKAGNRRPGKYNGQMAINYQDPDYGKRTLAMVTASVTVLD
                     KPPEQKDEPPSPPVGLSAKWMVDHIELFWSPPQSGTEPSGYNIFRAELDGDFPKTPLN
                     KAKVSETFYSDYEVKQGQAYRYRVQTVGKNGLLSDPGNEATVEIPVRLEKVENLVAKI
                     QGGTVTLTWESEQEVEFLIERNGEMIGSTFDKVFTDPFPPKTKLFYKVFPTKDGKMGP
                     EALAIVDLTPAPEPVPEPPKPPEPPQPKKVVIVFTVGKDTAAVDGKELPCQGTPYVKS
                     GRTMVPFRFLGESIGAEVSFEKDPATGRVAKVSYALSGKTITLSVDSRIAVVNGKEIT
                     LDAPPEIKDGRTYVPLRFVTDALGGEVSWDAKTRQATITYTKQDQL"
     gene            3691..4797
                     /locus_tag="PK717_04005"
     CDS             3691..4797
                     /locus_tag="PK717_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46858.1"
                     /translation="MRKEKACGLFLVLSIFSACCCPVGNSAGAVSISLFSIKGALSSA
                     NSASICSFEIAYPVRTTTNASEIEIRNVLEDKTERVGFGESFCSNPALSGSRLAFQAN
                     SGGSPKIVLFDRTTTSLLVVSEAGESSYSPVMKGNHLCWTFTKKSEIGQGIRLFGLSD
                     GKSVVLKNSDQSRIMSGPVINSLGVFWSQFFENGFAVFKYDFLTSTTQPLCKSSSVIS
                     GLSADGNILAYSKRPQNGFPVTGCIDGTGKVVVLDDVWSDKSDPSVSGTCVVWSVKKD
                     GASEIKVWNGSTNILANVKSAQCTPCVSEGNVVWHDQSGDGGSDIFGAEISGGGVFRV
                     TSGGGNKVLPRISGQVCIWLGGTDEGFDGAIITP"
     gene            complement(4849..>5515)
                     /locus_tag="PK717_04010"
     CDS             complement(4849..>5515)
                     /locus_tag="PK717_04010"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOR46859.1"
                     /translation="LLPPSLKDVYVSGLNQPPSASSTTIYTSEWGSKTPTTYPSICVT
                     LEKGVVTSVKTGPVAIPKDGYVLVYTGKNRERCDAKRWPKGYIKKGLHINIYWKAEKG
                     NEVDLGKWKECRFIFGGSPTVVRDGKPYWDLAADKHTNGAALSYEAKRSAFGTSPGKV
                     YFVVFTDEIKIQEESAVLIQLGVKNAFNLDGGCSSFLWFDGQAKSEACRELPMVVVAK
                     PRK"
BASE COUNT         1436 a         1232 c         1428 g         1419 t
ORIGIN      
        1 agcagatggg tgacaaagaa agcttgtgtt ggatatgggt gatccaccaa tggcgcactg
       61 ggcggcaagt gttattgacc agggtgaatt cccgtcacca gaggcaaaga ttgttgccga
      121 tccggagcca tcgtcacaaa ccaaagcagt cacctttttt gaaatctttg acaacaacta
      181 taaacacaaa tactggtctg cagtcatgac aaatgtctac acaaaaacaa tcgttgtcgc
      241 catcgatgat gatatgaaaa taagatatgt tagaccggta tctaccagta aatcacctga
      301 cccaacaata cttcagtatg gccaaattat tttcgatcca tacaatggtg ccaccccgga
      361 ccagctggct tttttgaacg caaggatccc gcaaaatgac aagtttacgc cttatgcaat
      421 agctgttagg tctgccgtgg caagtgccgt gaagttgttt gtaatagaca aagagggtta
      481 tcccgcttac cagaaaaagt atccctatgg gatactctcg ttccgtcccg gaacattatt
      541 tgtgcttcca aaaatgaaaa ctgacagcgg aaaagagata agcggggctt atttcaaggg
      601 caggccttat gtggtcattt ttgtttcttc gtgcgcatcc tgtaggaaaa gggcaaggga
      661 aatagcaaac attcttggaa agtttggaat aaagcctgac caggtaattt ttctgtcgtc
      721 atcggagaag gcaaagcttg aagagttccc aggtcaggtc aaggactcac acctttttat
      781 tgacctgaag aaggagcttg catctctgat gctctttgat tcaaccccct ctctgcttgc
      841 cgttgattct tcttcaaagg ttgttggcca gctggattcc ataaggctta atggagacgc
      901 agagctcaat gagctcctgc cgaaactgac aagatagaaa aaagacggac cgaagggcag
      961 aggtttccct ctgccctttt tatttggcaa attcaacctt ttccccaaaa tttcggtatt
     1021 tatatgtatg ggacgtttta caaaagtgct ggtttttttg ttgtctgtcc atattttgtt
     1081 tggccagacg gtctttgttg catccggagt tgaagacgag gggccaaaac cgtggacaca
     1141 gtatgcctct gaccacatgc actcatcgaa tgctcttgga cgatgggact tcaatgccta
     1201 tgacaaggaa tcctggaaac ttggcttgaa gggtgtcgag agcaggggaa gaaaccctgt
     1261 tgtcgaccag ggcaggcttt ttatcgggga ctcagacaaa atatactgca ttgacgtttt
     1321 caccgggaac atcctgtgga gtttcgaggt ttcctcaccg gtcgcaggga gctgtgccat
     1381 attgggtgac actgtcattg caccaatgaa caacaggctc cttgggcttt cgacggcaaa
     1441 cggggaggtc aggtggtaca atggagacgt tggggacaat atttcatcgc caaccgtcta
     1501 caaggacgac catggcggga agtggatgta tttcacaact catgacggga aaactggccg
     1561 ggcctataaa tacagtctga acaccggcaa gcttgcctgg aggattaaaa cagagtggcc
     1621 gtgcccgggg gcaatgggag taaacagtga ttatggctat gttggttgtg ccgcgcacta
     1681 caaggcgagc gtgctggatg atggctctgg tggtgtggtg gacggtgtgg aagtgggcac
     1741 ccaggctttt tctgccacca tggcgtttga caaatactgc atttgcacca tgtctggcgg
     1801 tgagattatc gcactgccaa gaccctacag caaaaaagac cccaagatat ttgataccgg
     1861 tggaatgacc tttgcgccac cgtgttcttt tggagacacg ctgataatag gggatgatga
     1921 gggcaggctg gtcaggttta atacagaagg aaaaataatc tgggacaaaa agatgccggg
     1981 acctgttact gacggttgca cggtcatggg tgaccatgtg cttgttcctg ttggttcaaa
     2041 ggggagcgac accgccggtg tttatatcat tgatggcgaa actggcgagc aggtcaaaaa
     2101 gataccgctc aaatcatctt atgttttcca gcccgtcgtg gcctgggaca ggatgtttgt
     2161 agaacatggc gagagcaaca attacagaaa ccggacactg agctgtttcg gaaaaccgcc
     2221 gaggacatca gatcaggagc caaagatcag ggtcgatggc ggaaagataa atgccgaggt
     2281 tggctacaag ggtgagacat caaggcaaat aaccattacc aatgatggga aaattcctct
     2341 cgaacttgtt cttcttggcg attcactcct cttcccgtcc aaggaagtac ttaagcttgc
     2401 gccaaaagaa aaagacacca tcagagtcgc aataaaagct ggaaacagaa ggccaggaaa
     2461 atacaatggc cagatggcaa tcaactacca ggacccggat tacggcaaaa ggacgcttgc
     2521 gatggtgact gcctccgtga ctgtcctgga taagccgcca gagcagaagg atgaacctcc
     2581 aagtccacct gttggcctgt cggccaaatg gatggtcgac catattgagc ttttctggtc
     2641 accgccacag tcaggaacgg agccttctgg ttacaacata ttcagggcgg aacttgatgg
     2701 ggatttccca aagacacccc tgaacaaagc gaaagtgtct gaaactttct attcggatta
     2761 tgaggtgaag cagggccaag cgtacagata ccgggtacag acagttggga aaaacgggct
     2821 cctctccgac cctggcaatg aagccactgt tgaaatacca gtcaggcttg aaaaggtgga
     2881 gaatcttgtt gccaaaattc agggaggaac agtaactctc acgtgggagt cggagcagga
     2941 ggtcgaattc ctcatcgaga ggaacggaga gatgataggg agcacttttg acaaagtgtt
     3001 cactgaccct ttcccgccca aaacaaagct tttttacaaa gtctttccaa ccaaagacgg
     3061 caagatgggc cctgaggcgc ttgcaatcgt tgatttaacg cctgcgccgg agccagttcc
     3121 ggagccacca aagccgcctg aaccgccaca gccaaaaaag gtagtgatag tttttactgt
     3181 tggcaaggac actgcagcag tcgatgggaa agagctaccc tgccagggaa caccctatgt
     3241 caagagtggc aggacaatgg tgccgttcag gttccttggc gagtccattg gtgcagaggt
     3301 ctcgtttgaa aaagaccctg caactggcag ggtagcgaaa gtgtcatacg cgctttctgg
     3361 aaaaacaata accctttcag ttgactccag gattgctgtt gtaaacggaa aagagatcac
     3421 gcttgatgcg ccaccagaaa taaaggacgg caggacttat gtccccttga ggtttgtgac
     3481 cgatgccttg ggtggtgaag tttcctggga cgcaaaaacc aggcaggcca caataaccta
     3541 tacaaagcaa gatcaattgt aaacaatttg taaacgattt tgtctccagt ttaggttgtc
     3601 tttgagagat aaaacccgga ctcaattgta ttttttcaat ttattgccag tctattgacc
     3661 cttccctcaa attggggttg aatatcaggc atgaggaaag aaaaggcctg tggccttttt
     3721 ctggtccttt ctattttttc tgcctgttgc tgcccggttg gcaatagtgc tggggcagtc
     3781 agtatttctc tgtttagcat caagggtgct ctctccagtg caaactccgc ttcaatctgt
     3841 tcttttgaaa ttgcctatcc ggtcaggacc accacgaatg cctctgaaat tgaaatcagg
     3901 aacgttctgg aagacaagac ggagagggtt ggttttggtg aatctttttg ctccaatcct
     3961 gcactttctg gcagcaggct agcatttcag gcaaattcgg gcggttcacc aaagatagtc
     4021 ctctttgaca gaaccacaac cagccttctg gttgtttcgg aggccggcga atcgtcctat
     4081 tcccctgtaa tgaagggtaa tcatctttgc tggacattca caaaaaagag tgagattggc
     4141 caggggataa ggctctttgg cctttcagac gggaagagcg ttgtgctgaa aaattctgat
     4201 caatcaagga ttatgtcggg acctgtcata aatagccttg gtgtcttctg gtcgcagttt
     4261 tttgaaaacg gttttgccgt tttcaaatat gattttttga catccacaac ccagccactg
     4321 tgcaaaagtt cttcggtcat ttcaggcctt tcggcagatg gcaacattct tgcctattcc
     4381 aaaagacccc aaaacggttt tcctgtgaca gggtgcattg atgggacagg aaaagtggtt
     4441 gttcttgatg atgtctggag tgataaatct gacccttccg tttccgggac atgcgttgtc
     4501 tggtctgtga aaaaagatgg tgcctctgag ataaaagtct ggaatgggtc aacaaatata
     4561 ctggcgaatg taaaatctgc gcaatgcact ccgtgtgttt ctgaagggaa tgtggtctgg
     4621 catgaccaga gcggtgatgg cgggtcagac atatttggtg ccgaaattag cggtggtggc
     4681 gtattcaggg tcacatctgg tggtggaaac aaggttttgc cgagaatctc gggccaggtg
     4741 tgcatctggc ttggagggac cgacgaaggt tttgacgggg cgatcataac cccatgaaat
     4801 taaaaaaggc cgtgccttat ggcacggcct tttgacagct tcagtctgtc actttcttgg
     4861 ttttgcaaca acaaccattg gcaattccct gcatgcttcc gattttgcct ggccgtcaaa
     4921 ccacaggaac gatgagcagc caccatcaag attgaaagcg tttttgactc caagctgtat
     4981 cagcactgca gattcttcct gaatttttat ctcgtctgtg aaaaccacaa aatagacttt
     5041 tccgggactt gttccaaatg ccgacctttt tgcttcgtag gaaagggccg ctccatttgt
     5101 gtgtttgtct gccgccaggt cccaatatgg cttgccgtcc ctgaccacag ttggcgaacc
     5161 accgaatatg aacctgcatt ctttccactt tccaagatcg acctcgtttc ccttttcggc
     5221 tttccagtag atgtttatgt ggaggccttt tttgatgtat cctttgggcc acctttttgc
     5281 atcacacctt tccctgtttt tgccggtgta tacgaggaca tacccgtctt tggggatggc
     5341 aacagggcct gtcttgacgc ttgttacaac acccttttca agggtgacgc agattgaagg
     5401 gtaagttgtt ggcgtttttg atccccattc ggaagtgtag attgttgtgg agcttgcaga
     5461 tgggggctgg ttcaatccgc ttacgtagac gtctttcagg gacggcggaa gtagg
//
LOCUS       DAOSRI010000102         5500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13527_length_5500_cov_3.758861, whole genome shotgun sequence.
ACCESSION   DAOSRI010000102 DAOSRI010000000
VERSION     DAOSRI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13527_length_5500_cov_3.758861"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK717_04015"
     CDS             complement(<1..242)
                     /locus_tag="PK717_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOR46860.1"
                     /translation="MNLESLYKLGVTIGIENDPRGREGVQRDLELSKKTYEALKDSEK
                     DYFDTVSLWNPYPDSRLQAVKDPDADIRELMVGIDIG"
     gene            336..1115
                     /locus_tag="PK717_04020"
     CDS             336..1115
                     /locus_tag="PK717_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46861.1"
                     /translation="MRKKGLALFALLVLLTPFLCCCGEVDDGRPKPIDTAKEFISEAL
                     NGKTDEAYKLLSKKQRMLTPLDTFKDITKERATGRTLGESGLNLEAQNQVVDGNKAFI
                     YGESEFNGKRTPFILPLVFENGRWLLDHPSIQLENASEKGFYFNLNLVNDAKNYFDKL
                     LDGDAKYLWENTSSKIKNGISFEDFMAMTVATQTGKKPREEGFVITIKQAYSNEEDEG
                     FIIGTLTMTGSQSQIALSERFIFEEGKWVADFIKLETEIGK"
     gene            1406..2509
                     /locus_tag="PK717_04025"
     CDS             1406..2509
                     /locus_tag="PK717_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46862.1"
                     /translation="MKKFIVIFIIALLIFPISGLGTSFATPVPLRGYIQGVELSNLKV
                     VVDGRDVGLLPSTQVFYLNGQKASIVDLAQGQFIEAKGSVQNGNFTAGLLEIVPSSQI
                     KQMTCYVQKNENGICSLSTGLRVSFDQGVNLEGSAPPKESSVIKIIGYTKRNNIFTPY
                     KVQKLFEDGKTYHLNGKLTIKEKEYLILEGKYFVFLEENSIFLDEEGMSTSFSSIHTG
                     NKVRLTCECRNGLLYASHMESFDVVEVVVTPDSNVAFINRLPCHLSQKVTKSEELIMI
                     PSLDIFRECGIKVIYNKSKNTLTTDCHGMFTTCDLQTGIVSRDGFETQHRIKPQVQNS
                     DVILPLPIFLAMICHTDPFLYPINDDPFSILYY"
     gene            2543..3757
                     /locus_tag="PK717_04030"
     CDS             2543..3757
                     /locus_tag="PK717_04030"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOR46863.1"
                     /translation="MKASIIAVGWEVLMGEVKNTNTEYISSKLRELGVETIFSAVVGD
                     DLEEASKIISFCIENSDVVFTTGGLGPTPDDLTREAVAKSLGIGIAENSQAKQWIEGY
                     FQKTGMSMPQINYVQAMLPDGSEPVWNGLGTAPGVYLEKSGKKIVLLPGPPREMAPML
                     DAILPRIASPTQKIYSRDFHVYGISEAAMAERIEGLLARKEKPLVAPYASLGHIRLRV
                     WGEYKNEEEFGNDLLETEKELKNKLSMHLFSDTIEAELGKILVGKKLTLSCAESCTGG
                     LVAKTITDVPGSSGYFMGGCVSYSNQAKMDILGVKPETLEAHGAVSEQTAIEMAEGCA
                     RVFGTDCAISTTGIAGPGGGTPDKPVGLVWMGFVWPGGSKTIKRIFGGSRDDVRTRTL
                     MTVLFELAKEIR"
     gene            4038..>5500
                     /locus_tag="PK717_04035"
     CDS             4038..>5500
                     /locus_tag="PK717_04035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOR46864.1"
                     /translation="MAKSKKIENGNNGATLGFETKLWQAADKLRNNMDAAEYKHVVLG
                     LMFLKYISDAFEEHHLELTNEDGADPEDPDEYRAKNVFWVPKEARWSNLQANAKQPAI
                     GKMVDEAMIAIEHDNRVLKGVLPKDYGRPALDKQRLGELVDLISGIGLGSPENRSRDI
                     LGRVYEYFLSMFASAEGKRGGEFYTPRCVVRVLVSMLAPYKGRVFDPCCGSGGMFVQS
                     EEFIENHGGKVGDISIYGQESNHTTWRLAKMNLAIRGIDANLGSENADSFHHDLHSDL
                     KADYILANPPFNMSDWGGERLREDKRWKYGIPPVGNANFAWVQHFIHHLSPVGTAGFL
                     LANGSMSSNTSGEGEIRKNIIEADLVDCMVAMPGQLFYQTQIPVCLWFIARDKQGRGA
                     SGKRYRNREEEVLFIDARKLGTMVDRVHRELSDEDIQMITDTYHAWRGDSDCKKKYED
                     IPGFCKSARLEEIQSHDYVVTPGRYVGSAPQEDDGEP"
BASE COUNT         1659 a         1167 c         1263 g         1411 t
ORIGIN      
        1 ccaatgtcta ttccaaccat caactctctt atgtcggcat ccgggtcttt cacggcctgc
       61 aatcttgagt ctgggtaagg attccagagg gaaacggtat caaaataatc tttctcgctg
      121 tctttgaggg cctcataggt ctttttcgag agttccaggt ctctctggac accctccctg
      181 cctctcgggt cgttttcgat cccgattgta acacccagct tgtatagcga ttctagattc
      241 atttttcctc cttttttgta cttctcaatg ctaccataaa aatccgtcgg cgtgtagtgt
      301 tgaacttgtt gccatccaca ggtagaatct cacccgtgag aaaaaaaggc ttggcgttgt
      361 ttgctcttct tgtgcttctt acccctttcc tgtgctgttg tggtgaggtt gatgatggca
      421 gaccaaaacc aatcgacacg gcaaaagaat tcatatctga agcgctaaac ggcaaaacag
      481 atgaggctta caagctgcta tcgaaaaaac aaaggatgct cacacctctg gatactttca
      541 aagacataac aaaagaacgc gcaacaggca gaacacttgg cgaatctggt ctcaatcttg
      601 aagcccaaaa ccaggttgtg gatggaaaca aggctttcat ttatggggag tctgaattca
      661 acggtaagag aactcccttc attcttccac ttgtgttcga aaatgggaga tggctcctcg
      721 atcatccctc aatacagctt gagaatgcct ctgaaaaggg tttctatttc aacttaaacc
      781 tcgtcaatga tgctaaaaat tactttgata aactcctgga tggcgacgca aaatatcttt
      841 gggagaacac aagctccaaa atcaaaaacg gtatttcttt tgaggatttc atggctatga
      901 ctgttgcgac acaaactggc aaaaaaccaa gagaggaagg atttgttatc acgataaaac
      961 aagcttattc aaatgaagag gatgaaggat ttatcatcgg gacgctgaca atgacaggta
     1021 gccagagcca gattgcactt agcgaaagat tcattttcga ggaaggcaaa tgggttgctg
     1081 attttataaa gcttgagacg gaaataggaa aataattttt cggcatattt tcggattatt
     1141 ttaggaccat ttgtcatatt aattttttac aatatgctcc aaaggctcat aacacttatt
     1201 gacataaaaa ctctgttatg atcattattg ataatgccaa aatacaaaaa taggaccatc
     1261 aaaaccgcaa ttgcaattct aaccgcttta aatcaacatc aaaccaaaca tcatcaagtt
     1321 aaatcagaca tcatatggtt atatctttat atgcccaacg cttgtttttg tgatttatat
     1381 tgctaaattt ttatacaccg acttgatgaa aaaatttata gtcattttta ttattgcttt
     1441 actcattttt cctatatccg ggttaggaac atcttttgcc acacctgttc cactgagggg
     1501 ttatattcaa ggggtagaac tttcaaacct caaggtggtg gtcgatggca gggatgtcgg
     1561 actactgcca agcacacagg ttttctatct aaatggccaa aaagcctcaa tagtggacct
     1621 tgcccaagga cagttcatcg aggcaaaagg atcagttcaa aacgggaatt tcacggccgg
     1681 cctgcttgag atagtgcctt caagccagat aaagcagatg acctgctatg tgcaaaaaaa
     1741 tgaaaatggc atttgctctc tttcaacagg tctgagggtt tctttcgacc agggtgtcaa
     1801 tcttgaagga tcagccccac ccaaagaatc atcggtaatc aaaataatcg gctacacaaa
     1861 aagaaacaat attttcacac cctacaaggt ccaaaagctt tttgaagacg ggaaaacata
     1921 tcacctaaat ggaaaattga ccataaaaga aaaagaatat ctcatactcg aaggcaaata
     1981 ctttgtcttt cttgaagaga acagcatatt cctcgatgag gagggcatgt caacctcatt
     2041 ttcttcaatc cacacaggca acaaagtcag actcacctgc gaatgcagga acgggctcct
     2101 atacgcttca cacatggaat catttgatgt tgtggaggta gtagtaacac ctgattcgaa
     2161 cgtggctttc ataaacagac tcccatgcca tctgtcccaa aaggtcacaa aatcagaaga
     2221 actcattatg ataccgtccc tggatatctt tagggaatgc ggcataaaag tcatttacaa
     2281 taaatcaaaa aacacactta caacagactg ccatggcatg tttacaacat gcgatcttca
     2341 aaccggaatt gtttcaaggg acggttttga aacacaacac agaatcaagc cacaggtgca
     2401 aaatagtgac gtgatactcc ccctgccaat cttccttgcc atgatatgcc acactgatcc
     2461 cttcctgtat ccaatcaatg atgacccttt ctccatcttg tattattgac gcaaaataaa
     2521 caagggttta cattcctgtg tcatgaaagc atcaataatt gctgttggtt gggaagtcct
     2581 gatgggtgag gtcaaaaaca ccaataccga atacatttct tcaaagctca gggagcttgg
     2641 ggttgaaacc attttttcgg cagtggtcgg agacgacctt gaagaggcat caaaaataat
     2701 ctctttctgt attgaaaact cggatgttgt cttcacaaca ggcggactcg ggccaacacc
     2761 tgacgacctc acaagagagg ccgttgcaaa atcgcttggc atcggcattg cagaaaacag
     2821 ccaggcaaag caatggattg agggttattt ccagaaaaca ggcatgtcca tgccacaaat
     2881 aaactatgtc caggcaatgc ttcctgacgg atcagagccg gtctggaacg gtcttggaac
     2941 agcgccaggc gtgtaccttg aaaaatctgg caaaaaaata gtcctgctcc ctgggccacc
     3001 aagagagatg gcgccaatgc ttgatgccat tctgcccagg attgcctccc caacccaaaa
     3061 aatatatagc agggactttc atgtgtacgg catctcggag gcagcgatgg ccgaaaggat
     3121 tgagggtctg ctcgcacgca aggaaaaacc cctcgtggcg ccatacgcca gccttggcca
     3181 catcaggctg agggtgtggg gtgaatacaa aaatgaggaa gaattcggca acgaccttct
     3241 ggaaactgaa aaagaactga aaaacaagct ctcaatgcat cttttttctg atacaatcga
     3301 ggcggagctt ggcaagatac ttgtggggaa aaagctgacc ctttcatgcg cagagtcctg
     3361 cacgggcggc ctcgtcgcaa aaaccataac agatgtcccg ggatcatccg gatatttcat
     3421 gggcggctgt gtgtcgtatt ccaaccaggc aaaaatggac atccttggtg tgaaacctga
     3481 aacgctcgag gcccacggcg cagtctccga gcaaacggca atcgaaatgg ccgaggggtg
     3541 cgcaagggtg tttggaacag actgtgcaat ctccaccaca ggcattgccg gcccgggtgg
     3601 tggaacacct gacaagccgg ttggtctggt ctggatgggt tttgtctggc caggtggaag
     3661 caagaccata aagaggattt ttggcggctc gcgggacgac gtgaggacaa gaacgctcat
     3721 gacagtgctg tttgaactgg caaaagaaat ccgctaaatc cgtaatttct cacaatttta
     3781 cagcaggatt tgaaatttgt tttgacaaat ttattctgtt ggaagtttgc aaaatatcat
     3841 ttgagttgtg gatttcatcg tatagtttca tattgggaaa tagcattgat atttattaat
     3901 actggagaaa tatatgggtt gattgttcta aaatctattt tcagcacctg tcctgaaagg
     3961 ttttaacata tgcaaggaag cgtaaaaggt tataattgga gaaattacca aggggttcta
     4021 atctctggag gttttgagtg gctaaatcaa aaaaaatcga aaatggaaac aatggagcaa
     4081 cgctaggctt tgaaacaaag ctctggcagg cggctgataa gttgcgcaac aatatggatg
     4141 ccgccgaata taaacacgtt gttcttggtc tcatgttttt gaaatacatc tccgacgcat
     4201 ttgaagagca ccacctagaa cttacaaatg aagacggtgc cgatcctgaa gatccagatg
     4261 aatatcgtgc caaaaatgtc ttttgggtgc caaaagaagc cagatggtca aatcttcagg
     4321 caaacgcaaa acaacctgct attggcaaga tggttgatga agcgatgata gccatcgagc
     4381 atgacaacag ggttctgaaa ggtgttctgc caaaagacta tggcaggcct gcgcttgaca
     4441 aacaacgcct cggagaattg gtagatctga taagtggaat aggtcttggt agcccagaga
     4501 acaggtcaag ggatattttg gggcgcgtat atgaatactt tctctccatg ttcgccagtg
     4561 cagagggcaa aagaggcggt gaattctaca caccacgatg cgttgttcgt gttttagttt
     4621 cgatgctggc accatacaaa gggcgtgtgt ttgacccatg ctgtggttcc ggcggaatgt
     4681 ttgtccaaag cgaagagttt attgaaaacc atggtggcaa agttggcgat ataagcattt
     4741 atggccaaga gtcaaaccat accacatggc gacttgccaa aatgaatctg gcaattcgag
     4801 ggatagatgc aaatcttggt tcggagaatg ctgatagttt tcatcatgat ctgcattcag
     4861 atctcaaagc cgattatatc ctagccaatc caccatttaa catgagtgat tggggcggtg
     4921 aacgcctgcg tgaagacaaa cggtggaaat atggcattcc tccagtggga aacgcaaatt
     4981 ttgcatgggt gcagcatttt attcatcatc tgagtccggt cggcaccgca ggttttttgc
     5041 tagcgaatgg aagcatgtcc tcaaacacaa gcggagaagg cgaaatacgc aagaatatta
     5101 ttgaagcaga ccttgtggac tgcatggtgg ccatgccagg acaattgttc tatcaaacac
     5161 agataccagt ctgtctttgg ttcattgcac gcgacaagca agggagaggt gcgagcggca
     5221 aacgttatcg aaacagggag gaagaagtcc tgttcattga tgcacgaaaa cttggcacaa
     5281 tggtagatcg tgtccatcgc gagctgagtg atgaagatat tcagatgata actgatacat
     5341 atcatgcctg gcgtggtgat agtgattgca agaaaaaata cgaggatatt ccgggatttt
     5401 gcaaaagtgc gagactggaa gaaatccaga gccacgatta tgtagtaacc cctggtcgct
     5461 atgtaggctc ggccccacaa gaagatgatg gcgaaccttt
//
LOCUS       DAOSRI010000103         5489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13551_length_5489_cov_4.081892, whole genome shotgun sequence.
ACCESSION   DAOSRI010000103 DAOSRI010000000
VERSION     DAOSRI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13551_length_5489_cov_4.081892"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1211
                     /locus_tag="PK717_04040"
     CDS             24..1211
                     /locus_tag="PK717_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46865.1"
                     /translation="MRRQTLTLVFLFIFFACCGCSVPGVQKIWGSVRRAQVAPVSFGK
                     TMFVDQEIFGLVGADGTPVWTSTIKGAKRVFCRAIGDYLFAFGDSRIIEKVSIDGKSA
                     WRFEAPQGSGVWLLSSSKKVCVLAYDSDTDYDLNPIQLIGLSAADGQESWRLDDRDYQ
                     ACLMVPDSNFFTDIFVAPFSRGETVWFDGINSADGNQVWRTSWGIPRPGHPLVLAQDE
                     TKFWAWKAEGEGVRVAVFSRTDGHEIGSSAGPSGFLADTSFVGENVFIGFKDGKYKIN
                     ALGKYEKFGMEYFPICQVPDYKNLAVCISDDCSSFVVMNIENGKIFNGFKINACYSAV
                     PGYSFGEFYLVPEVSPYDHQTFRCQFIGDREIAATKIPSDLKGLGKTRSIKDLFHDLM
                     KSR"
     gene            complement(1214..2362)
                     /locus_tag="PK717_04045"
     CDS             complement(1214..2362)
                     /locus_tag="PK717_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46866.1"
                     /translation="MRGKAGKFNFTLFLAVVLLLGTLSLTTFLWSIFIDCRVSAPTEK
                     SVPIMSLRDIPTDGKSTPNVAPLTYPESINAQIMGIISGLGIQTYDLGLVGYWEPDSP
                     FNEAKRGREVVLIGISSGKYQGTLIITTPKGPIIATPQMLYTPDPPMWEENYALITQR
                     YSEWKAQLEKNSKLVSDESFKFPDKDGLLAWEFGGKTCVLSKDGLSFFDATIEDTEKF
                     LSKQNHIPFAPPALHLAITVGLLSLFALALFIKGIQDIGIASIFCAACLGAIGGASLF
                     LQQYVPGTLLPLASVLSVGIVWTIPIIAISSAFALDLSLGWFWLVYSILSSGVVLQWI
                     FPPDYVWFPIEGIIFILMLIVFISMAIKPKMPIKKELFPQTPGQNGIA"
     gene            2489..2575
                     /locus_tag="PK717_04050"
     tRNA            2489..2575
                     /locus_tag="PK717_04050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2523..2525,aa:Leu,seq:caa)
     gene            complement(2653..3327)
                     /locus_tag="PK717_04055"
     CDS             complement(2653..3327)
                     /locus_tag="PK717_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46867.1"
                     /translation="MKSREIIAILGTAIICTSCGGGAVNTPTNNGNDEQSGFKIPGPL
                     PRVGIVFDVKKDVGTFNLSILPEETGNSNRLIQVDFSSAHIVNSKGAEMGKEALIDSQ
                     TVKVDGSFTASDKIKATTVEIESEEATVLKLKDSAIVGVLDVPEIVRENYAEMFPADF
                     GEKNWIVGTKSGKTPKTFTLEKNSWKMVIVEDPKTESQFEVTVSENKKQLLSCDILKG
                     RKLIKK"
     gene            complement(3314..3967)
                     /locus_tag="PK717_04060"
     CDS             complement(3314..3967)
                     /locus_tag="PK717_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46868.1"
                     /translation="MRKIALVFVACALLASCGQAGQNPEPEKKTLEFPSTFKVESKYT
                     PGEDIVVSKEGSNYNMFFGEGAKVFFDDKEVEAQSILNGQTIYVEGVLENGKIKATRL
                     VITEWPGRKEKGKDIIIDPKMVPEMVSQYLSEKHSEFGVGRNTVWTKKAKLGNPPPGR
                     LMDTYQYGTMLMVVTYDEMEGTRSFTVLITPGSQSPASWSGTINSDGSIEEFLYEKP"
     gene            4192..4677
                     /locus_tag="PK717_04065"
     CDS             4192..4677
                     /locus_tag="PK717_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46869.1"
                     /translation="MEREQQLQVFLDLMIEAVCRAYSYIVGVEQLKMFVSKTCEAMFM
                     IAEEKGYGSVSGSNLQDVAKSIAAMLSQIDPREKDWTTTSNGDKLGLTISKCQYAHMC
                     SEMFSEIISSGRVDKTKCPCIMAEFIAGGARNVGIKARTTMQSFAPGHKCVSEVIKME
                     M"
     gene            4679..5227
                     /locus_tag="PK717_04070"
     CDS             4679..5227
                     /locus_tag="PK717_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46870.1"
                     /translation="MGIRDEMISILKINDENGSVMFGPVTVAYHCDTFNTRIIKGFED
                     TLGYEHARELVLTTTEISTYDPLSDMLNAKTQGMSFEDSLKVAFDFYRVIGCGSFELV
                     ELKGKGGKVKGVTFTAEGWLENETKWNWNVRKEPVCHVEAGLIATVWEVLNKLPKGSV
                     SVKEISCKSMGNPECIFEVEVK"
     gene            5228..>5489
                     /locus_tag="PK717_04075"
     CDS             5228..>5489
                     /locus_tag="PK717_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46871.1"
                     /translation="MTIDQAGIRKAVLDFGLFGDEKTGILRRFDIMLSAFPTAFYVDR
                     ELALVKAMGGEPSRKLAEKVLIDAAQWSANQIFSGVMASPEWK"
BASE COUNT         1493 a         1101 c         1280 g         1615 t
ORIGIN      
        1 aggctgaaaa tttgggagga gaaatgagaa ggcaaactct aacgctggtt tttcttttta
       61 ttttttttgc ctgttgcggg tgttcggtac caggtgtgca aaagatctgg ggatcggtca
      121 ggagagcgca ggttgccccg gtctcttttg gcaagacaat gtttgttgac caggaaatat
      181 tcgggttagt cggggctgat ggaacaccag tctggacttc tacaatcaaa ggtgcaaaga
      241 gggttttttg cagggcaata ggcgattacc tgtttgcgtt cggagactcg aggataatag
      301 aaaaagtcag cattgacgga aaatcagcct ggaggtttga ggcgccacag gggagcggcg
      361 tctggctttt gtcttcgagc aaaaaagtat gtgtcctggc ctatgacagt gatacggact
      421 atgatctgaa cccgatccag ctcatcgggc tctcggcagc tgacggacag gagtcctgga
      481 gacttgatga cagggattac caggcatgcc tcatggttcc ggactcgaat ttttttacag
      541 atatttttgt ggccccgttc tcaaggggcg agactgtatg gtttgatggc atcaactctg
      601 ccgatggcaa tcaggtctgg aggacttcgt ggggtattcc aagaccgggc catccgcttg
      661 tccttgcaca ggatgaaaca aaattttggg catggaaggc agaaggggaa ggtgtgaggg
      721 tggctgtttt ttcaagaact gacggccatg agattggctc ctctgccggc ccttcaggat
      781 tccttgctga cacctctttt gttggagaga acgtttttat tggtttcaaa gatgggaaat
      841 acaaaatcaa tgcccttggg aaatatgaaa aatttggcat ggaatatttc ccgatttgcc
      901 aggtgccaga ctataaaaac ctcgctgtat gcatctctga tgattgcagt tcctttgttg
      961 tgatgaatat tgaaaacggg aagatcttta acggtttcaa gataaatgcc tgctacagcg
     1021 cagttccagg atactctttt ggtgaattct accttgtgcc ggaggtctct ccgtatgacc
     1081 accaaacttt cagatgtcag ttcattgggg acagggaaat cgcagcaaca aaaatcccaa
     1141 gcgacctgaa ggggcttggc aaaaccagat caataaaaga cctcttccac gatttgatga
     1201 aatccagata attttaggct atcccgtttt gtcctggtgt ttgtgggaaa agctcttttt
     1261 tgattggcat tttgggtttt attgccatcg aaataaatac aatcagcatc agaatgaaaa
     1321 tgattccttc gatgggaaac catacataat ctggcgggaa aatccattgc aaaactactc
     1381 ctgatgaaag aattgaatag acaagccaga accagcccag tgaaagatca agtgcaaagg
     1441 cagatgatat tgcaattatc gggatggtcc atacgattcc aacggacaat accgatgcca
     1501 atggtaaaag ggttcccggc acgtattgct ggaggaaaag tgatgctcct ccaattgcac
     1561 caagacacgc agcacaaaaa atgcttgcaa ttccgatatc ttgaatgccc tttataaaca
     1621 gggccagcgc aaacagtgaa agcaagccta ctgttattgc cagatgcaaa gctggcggtg
     1681 caaacggaat atggttctgt tttgacaaaa atttttcggt gtcttcgatt gtggcatcaa
     1741 agaaagaaag accatctttt gaaagaacac aggttttgcc accaaattcc catgccagca
     1801 aaccatcctt gtccgggaat ttgaaggatt catctgatac caacttggag tttttttcaa
     1861 gctgggcctt ccactccgaa tacctctggg ttatcagtgc gtagttttcc tcccacattg
     1921 gagggtctgg ggtatagagc atttggggcg tggcaatgat tggccctttt ggtgttgtga
     1981 tgatgagtgt tccctggtat tttccagaag agattcctat aagaactact tctctccccc
     2041 tttttgcttc attgaatgga gaatctggct cccagtagcc cacaagtcca agatcgtagg
     2101 tctgtatgcc aaggccagat attattccca ttatttgtgc atttattgat tcggggtatg
     2161 tgagaggggc cacgtttggt gtggatttgc catctgtggg gatgtctcgg agtgacatta
     2221 tgggcacact tttttctgtt ggggcactca ccctgcagtc aataaatatg ctccaaagaa
     2281 aagttgtcag ggagagcgtc ccaaggagca atactactgc aaggaaaaga gtaaagttga
     2341 attttccggc ctttcctctc atttccaacc tcctataatg gaatcataac acaaaatggg
     2401 tctttggcaa caagatattt tttggcaacc acctttcctg gcaatgaatt gacactacaa
     2461 gcaatgccaa taaaatggtg caatggttgc cgaagtggcg gaactggcag acgcgacggt
     2521 ctcaaaatcc gttgaggtaa cactcttgag ggttcgagtc cctccttcgg caccatctgg
     2581 aaacagctac gaatagatat aaaaaagccc cgataaaatc ggggcttttt tatatctatt
     2641 atatatgtag ccttactttt ttatcaactt cctgcctttt agaatatcac aagataggag
     2701 ctgctttttg ttttcgctga ccgtgacctc aaattgggat tctgttttcg ggtcttccac
     2761 aatcaccatt ttccagctgt ttttttcaag tgtgaaagtt tttggtgttt ttccagattt
     2821 tgtacccacg atccagtttt tttcaccaaa atcggccggg aacatttctg cgtagttttc
     2881 cctgacgatt tctggtacat caagaacgcc tactatggca gaatctttta gtttcaaaac
     2941 agtagcttct tcggattcga tctcgactgt tgtagctttt attttatcgg aagcagtaaa
     3001 agatccgtca accttcactg tttgggaatc tatcaaggct tcttttccca tttcggcgcc
     3061 ctttgagttg actatgtgcg cggatgagaa atcaacctgg atgaggcgat tgctgttgcc
     3121 tgtttcttcg ggcagtatcg aaagattaaa ggtcccaaca tcttttttta catcaaacac
     3181 aatgccaacc cttggaagtg gcccggggat tttaaagcct gattgttcgt cattgccatt
     3241 atttgtgggg gtgtttactg cccctccacc acaagaggta cagatgattg ctgttcccaa
     3301 aattgctatt atttcacggc ttttcataca aaaactcctc tattgatcca tcactattta
     3361 ttgtgccact ccagctagct ggtgactgtg agcctggtgt aatgagcacg gtgaaacttc
     3421 ttgtgccttc catctcatca tatgtgacca ccatgagcat ggttccatac tggtatgtgt
     3481 ccatgagcct tccaggtggt ggattgccca attttgcctt tttagtccaa acggtatttc
     3541 taccgacgcc aaactcactg tgtttttcgg acagatattg agaaaccatc tctgggacca
     3601 ttttggggtc gattatgata tctttgcctt tttccttgcg ccctggccat tcagttatca
     3661 ccagacgggt tgcttttatc ttgccatttt caagcacacc ttcaacataa atggtttgcc
     3721 catttaagat tgattgtgcc tcaacttctt tgtcgtcaaa gaaaaccttg gcaccctcac
     3781 caaagaacat attgtagttt gagccttcct ttgaaacgac gatgtcctca cccggcgtat
     3841 atttgctttc aaccttgaat gttgatggga actcaagagt ctttttttct ggttctgggt
     3901 tttgcccagc ctggccacat gatgcaagca gtgcacatgc aacgaaaacc aaggctattt
     3961 ttctcatggg catttaatat cattaaacgt ttgcggcggg gttaaaaatg ttgatattat
     4021 tttttatatt gataaaaatt aatcacattg taaaaagtca attgtaaaaa aaatgttaac
     4081 ttcaccattg actattgaaa tgtgttttgt attgtatttg tggctaaaga ttaagccaaa
     4141 ttttctgttt tattaataca aattgtcact aacttaaaaa ggaggcattt cttggaaagg
     4201 gaacaacaat tacaggtttt tcttgatttg atgatcgagg cagtttgcag ggcctattcg
     4261 tatattgtgg gtgttgagca attaaaaatg tttgtctcaa aaacatgtga ggccatgttc
     4321 atgattgctg aagagaaagg ctatggcagt gttagtggat caaacctcca ggatgttgcc
     4381 aaatctattg ctgccatgct tagtcaaatt gatccaagag agaaagattg gacaaccaca
     4441 agcaatggtg acaagcttgg tctaacaata tccaaatgcc aatatgccca tatgtgttcc
     4501 gaaatgttct cagagatcat ttcttccgga agagtcgata aaacaaaatg cccatgcatt
     4561 atggcggaat ttattgcagg aggagcaaga aacgttggca tcaaagccag aacgacaatg
     4621 cagagctttg cacctgggca taagtgcgtt tcagaagtca ttaaaatgga gatgtgacat
     4681 gggaataaga gatgaaatga tatcaatttt gaaaatcaat gatgaaaatg gctcggtaat
     4741 gtttggccca gttaccgttg cttatcattg tgacacattt aacaccagaa ttataaaggg
     4801 ttttgaagat actcttggct atgaacatgc aagagagctt gttctcacta ccactgaaat
     4861 ttctacctat gatcctttgt ccgatatgct aaacgcaaaa acacaaggca tgtcattcga
     4921 agattccctg aaggtagctt ttgattttta cagagttata ggttgtggct cttttgagct
     4981 tgttgaacta aagggcaagg gtggaaaggt aaagggtgta acatttacgg cagagggatg
     5041 gctcgagaat gagacaaaat ggaactggaa cgtaagaaaa gagccagttt gccatgtcga
     5101 agctggtctg atagccaccg tttgggaggt actcaacaag ttgccgaaag gatcggttag
     5161 cgttaaggaa atttcctgca agtcgatggg caatcccgag tgcatttttg aagtggaggt
     5221 gaaatagatg actattgatc aggctggaat tagaaaagct gtcctggatt ttggactttt
     5281 tggtgatgaa aagaccggca ttcttcgtag gtttgatatt atgctttcag catttcccac
     5341 cgcattctat gttgacagag agctcgccct agtaaaggca atgggtggag aaccgtccag
     5401 aaaacttgca gaaaaagttc ttattgatgc tgcccagtgg tcagcaaacc agattttttc
     5461 gggagtaatg gcttctccag aatggaaga
//
LOCUS       DAOSRI010000104         5443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13692_length_5443_cov_4.400334, whole genome shotgun sequence.
ACCESSION   DAOSRI010000104 DAOSRI010000000
VERSION     DAOSRI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13692_length_5443_cov_4.400334"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PK717_04080"
     CDS             complement(<1..1637)
                     /locus_tag="PK717_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46872.1"
                     /translation="MRKAFLIVMPAILLLCSCGAPQDKNTQAPGIYRKATSQEMSLAV
                     TFLPEGWSFSSPIVVDIGQKYLFAEAVKYDKGGGKSERFMAFRIDGDLVLDVYDSLPS
                     RFRIVDDSGISKNHETKYKVESDLFPEDGKPVQELNGGAYVPVCWTDDDYPATWSPVY
                     IIRNDKVVSLPMWPKVLQTIVGPAKIGEENVLVSIELESLTRISASSEEPIYVPAIYA
                     PAIDRVMKKKDGIWVDACEEFQSYGQKKADEILDTPNGELDFENLRKVYLYGLVYGFK
                     DEAIRKIESRIESDPNLKNNPLGKDRVFIKKITSEDDYQPIYPGREEARLFLQPSPKR
                     MEAFRSAVDKKYVVEAVRMADSNSLFAITSTQGDDGQQEKALFFASFAGDSIASLYKT
                     SISGLATFGFMEGADLWGYEGLAPFPVYPVGVNEVGDFEHPRSIVFKVEEDKPVQVPF
                     EFEKGEVARGVKVVGEKAYIYAISEHFGSPFGIAYKDRLMEYSGDSFREIWPILSSMQ
                     GKAVSDDYEKNGLELLIDAKKALLSKEELTDYYSYYSA"
     gene            complement(1771..3219)
                     /locus_tag="PK717_04085"
     CDS             complement(1771..3219)
                     /locus_tag="PK717_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46873.1"
                     /translation="MSELVKKLQKKLPPGEFRIQVRIWTQWAFLALAAGPLFILAPIV
                     KQIPAPWYVCWSCPTAIAACPLGTLQHYTASGGFSFFAVGFLALIGVLIGRMTCGWTC
                     PMGFVQEMLYKAKRYGNYIVGGIIFVTTMFLAWFTPFLGLIRAPVGEGYGLGYLLSSG
                     NINAFIGWLLTYLSVALLVSVPSFLVEKKNKWSNFFWWLLFIVPFAGLFLAFYLTGFM
                     KSEPLTVFLVLGIVIPITLLCFMNVKKFSIPNNLATFMRWFFFIVPFIIFPYITRFVA
                     TDAGIKLLEGEVWWCKICPIGTITAGFPQMYLQLVPQGAFPLFGLTSTAFIGVSTYQG
                     IFGEARVMYFIKWVMTSGIIFAMVNSKRFFCKAVCPLGTFFTIFNWFSATRIKVDQAN
                     CRGKSCNWCLKHCPMDIAIYDPTSQWHCIRCLDCLGCPFGVVTHEVPVVFSWMVKKEE
                     KKVIPLKTPNPPNNPTFLQWANFINYTPPQAR"
     gene            complement(3216..3866)
                     /locus_tag="PK717_04090"
     CDS             complement(3216..3866)
                     /locus_tag="PK717_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46874.1"
                     /translation="MKLFKPANIIPIITGERGVLSDMRKWIYIPLALVCVFMIYWGIW
                     GTEMHKIEPVLQSRTTTTGTVSGLDAQNGKLTLNVDDKGKTAVWEVSFNPEKLKLFYI
                     DRKTEDGKETEKRVQVMYTEKPELLKDGITITATGEKKDGKIKSSVFHIGGIYNPPEL
                     VQRKENVTIYNTADKLCIACVGLGDSDLTRLLQIGIIFIGIMIIYAAVSYVTRRAA"
     gene            complement(3972..>5443)
                     /locus_tag="PK717_04095"
     CDS             complement(3972..>5443)
                     /locus_tag="PK717_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46875.1"
                     /translation="KYWSYDIKRPIVAAPCRYYNATYVGIGTEDGQVFCIRLGDGNAI
                     WDQKAAGPIKQAVIGINQNLVAATENGHIQAFFAFDGTVVADVDLKDNITTPPSYSSG
                     SIFVGTKSGRVVCVRPKQGSLSLRLDPSIVFISPTESKSVKIRLGSIEGDSNVYYLSN
                     SGFPCRCKLARNFMPSSTMKATDERTLDLKADPSAEPATYEYLVTAMSQSSSEARVSA
                     TGICIVVKPEDIMKSYMRFDTSKKNQLVVTVGYENQKFMKSFAGKIIYDQAILRPVET
                     KSLLEGPEYAPFFDLTIKGEVGTYMGFSPSKAKEAKADVFQITFDIIKSAKTNLKFSP
                     VSRTMSSYILPTTDTAVDVEVVRKMTEHVVKLQIGNVMATVDGKQEKLAIAPYIKSGR
                     TMVPLRFVGTALGSVVDWIAAEKSVLYTNSLPTGARTIKLWIGKKVALVDGKEVAIDP
                     PPELVKGNTCVGLSFVSTNFGAKIAYDSKTKTVTINFEN"
BASE COUNT         1433 a         1275 c         1237 g         1498 t
ORIGIN      
        1 gccgagtagt acgagtaata atcggtcagt tcttctttgg acaataaagc tttttttgcg
       61 tctataagaa gctccaggcc gttcttctca taatcatcac taacagcctt tccctgcatg
      121 ctacttagta tgggccagat ttccctgaat gagtcgcctg aatactccat cagcctgtct
      181 ttgtaggcaa ttccaaatgg tgacccaaag tgctcgctta tggcgtaaat gtaggccttt
      241 tcacccacaa cctttacacc tcttgccact tcgccctttt caaattcaaa agggacctgg
      301 acaggcttgt cttcttcaac cttgaaaaca atcgagcgcg ggtgctcaaa atcgccaacc
      361 tcattgacac cgacaggata aaccggaaat ggcgccagac cctcataacc ccagaggtct
      421 gccccttcca taaacccgaa ggtcgcaagg ccggaaatgc tggttttgta aaggctggca
      481 atggaatctc cggcaaaaga ggcgaaaaag agggctttct cctgctggcc atcatccccc
      541 tgtgtgcttg taatcgcaaa aagcgagttg ctatcagcca ttctgacggc ttccaccaca
      601 tactttttgt caacagctga cctgaaagct tccatccttt ttgggctagg ttggaggaag
      661 agccttgctt cctctcttcc aggatatatc ggctgatagt cgtcttctga tgttattttt
      721 ttgatgaata ccctgtcctt gcccaaaggg ttgtttttca aattggggtc gctttcaatc
      781 ctggattcaa tcttcctgat tgcctcgtcc ttgaaaccat agacaagacc ataaagatag
      841 acctttctca ggttctcaaa atcaagttcc ccatttggcg tgtcgagtat ttcatcggcc
      901 ttcttctggc catagctctg gaactcctcg cacgcatcta cccagatgcc gtcctttttc
      961 ttcatcaccc tgtcgattgc aggagcataa attgctggaa cataaattgg ctcttcggag
     1021 gatgcactga tccttgttaa agactcaagc tcaatggaaa ccaggacgtt ctcttctcca
     1081 atcttggcag ggccaacaat ggtttgtaaa accttgggcc acattggcag ggaaaccacc
     1141 ttgtcgtttc tgataatgta gactggcgac catgttgccg gataatcatc atcagtccag
     1201 cagacaggaa cgtatgcccc accattaagt tcctgtacag gttttccatc ttccgggaaa
     1261 aggtcagatt caaccttgta tttggtctca tggtttttgg aaatgccgct gtcatcaact
     1321 atacgaaatc gcgaaggcag cgaatcataa acatcaagca ccaagtcccc atcaatcctg
     1381 aatgccatga atctttcact ctttcctcca cccttgtcgt acttcacggc ttcagcaaag
     1441 aggtatttct ggccgatatc gaccacaatg ggggaagaaa aactccatcc ttcaggtaaa
     1501 aaagttactg caagggacat ttcctggctg gttgccttcc tgtaaatacc tggagcctgg
     1561 gtgtttttgt cctggggcgc accacaggag cacagaagca gaatagctgg catgacaatc
     1621 aagaatgctt ttctcaagtt ccctccttca aacgcttttc attgtagttt atcagccaag
     1681 caaaaatagt ttcaatatcc ataaacaaaa ggtatcaaaa aattcaaaag gccggcattt
     1741 cagtgccggc cttttttgat gttgggtttt ttatctagcc tgtggtgggg tgtagtttat
     1801 aaaattagcc cactggagga aagttgggtt gtttggaggg ttcggtgtct tcagaggtat
     1861 tactttcttt tcttccttct tgaccatcca agagaacacg acagggactt catgtgtcac
     1921 aacgccaaac gggcaaccaa ggcaatccag gcacctgatg cagtgccatt gtgatgttgg
     1981 atcgtagatt gcaatgtcca tcgggcagtg tttgagacac cagttgcagg acttgcccct
     2041 gcagttcgcc tggtcgacct tgattctggt tgccgagaac cagttgaaga tggtaaagaa
     2101 agtgccaagt ggacaaactg ctttgcagaa gaacctcttt gaattgacca ttgcaaagat
     2161 gattccggag gtcattaccc attttatgaa atacattacc ctggcctcac caaaaatgcc
     2221 ctggtaggtt gaaacgccaa tgaaagcggt cgaggtgagt ccgaagagcg ggaaggcacc
     2281 ctgtggcaca agctggaggt acatctgcgg gaaacccgcg gttattgttc cgatgggaca
     2341 tatcttgcac caccagacct cgccctcaag cagcttgatg ccggcatctg tggcaacaaa
     2401 tctggttatg tacgggaaga tgatgaacgg gacgatgaag aagaaccatc tcatgaatgt
     2461 tgccagattg tttggaatgg agaatttctt gacgttcatg aagcaaagga gtgttatcgg
     2521 gatcacgatg ccaaggacaa ggaaaactgt gaggggttcc gatttcatga aacccgtgag
     2581 gtagaacgca aggaaaaggc ctgcaaatgg aacgatgaaa agcagccacc agaagaagtt
     2641 tgaccacttg tttttctttt ccacaaggaa agatggaaca gaaacaagca acgcaacgga
     2701 caaataggtg aggagccagc caataaaagc attgatattg cccgaggaga ggagatagcc
     2761 gaggccgtat ccctcgccaa ccggagccct gatgaggcca agaaaggggg tgaaccaggc
     2821 caggaacatc gtcgtgacaa aaattattcc accaacaatg taattgccat atctctttgc
     2881 cttgtaaagc atttcctgga caaaacccat tgggcaagtc catccacatg tcattctgcc
     2941 aataagaacg ccgataaggg caaggaaacc aactgcaaag aagctgaaac cgccagaggc
     3001 ggtgtaatgc tggagtgtcc cgagtggaca ggcagcaatc gctgttgggc atgaccagca
     3061 aacataccaa ggggccggta tctgttttac gattggtgca aggatgaaaa gcggtcccgc
     3121 tgcgagggcc aggaaagccc actgggtcca gatcctgacc tggatacgga actcaccggg
     3181 tggaagtttt ttctgaagtt ttttgaccag ctcgctcatg ccgccctcct tgtcacataa
     3241 gatactgcag cataaataat cattatgcca atgaagatga tgccaatctg aagaagtctc
     3301 gtaagatcgg aatcaccaag cccgacacaa gcaatgcaga gcttgtcggc cgtgttgtag
     3361 atggtcacgt tctcttttct ctggaccagc tcaggtggat tgtaaattcc accaatgtgg
     3421 aaaactgacg attttatctt gccatctttt ttctcaccgg tagctgttat agtgattcca
     3481 tctttgagca gctcaggctt ttcggtgtac attacctgaa ctcttttttc agtctcctta
     3541 ccatcctcag tttttctatc tatataaaaa agtttcaatt tttcaggatt aaatgacact
     3601 tcccaaactg cagtcttgcc tttgtcgtca acattaaggg tcaactttcc attttgggca
     3661 tcaagaccag agacggtgcc tgtggttgtt gtcctgctct gaagtaccgg ctcaattttg
     3721 tgcatttctg tgccccatat gccccaataa atcataaata cgcatacgag ggccaacggg
     3781 atataaatcc attttctcat gtctgacaat acaccccttt cgcctgttat gattggaatt
     3841 atatttgccg gtttgaaaag cttcaagtgt tttgtacttt ttaggttttg gggaagcagc
     3901 aaattgaccg aaaataaaaa agggcctcag ttgaggccct ttttttaaat cactagatca
     3961 atttttactg attaattctc aaaattgatt gttaccgtct ttgtctttga atcataggca
     4021 atctttgcac caaagttggt tgagacaaac gagaggccaa cacaggtgtt gcctttgacc
     4081 agctccggcg gcgggtcgat tgctacttcc ttgccatcaa caagggcgac cttcttgcca
     4141 atccaaagct ttatggtcct tgcgccagtt ggcagcgagt tggtatagag aacagatttt
     4201 tctgcagcaa tccagtccac cactgatcca agtgcagtgc caacaaaacg tagtggaacc
     4261 attgttcttc cactctttat gtaaggtgca atagcaagct tttcctgctt gccgtcaact
     4321 gtggccatca catttccaat ctggagtttt acgacatgct cagtcatttt gcgcacaacc
     4381 tcgacatcaa cggctgtgtc agtggttgga agaatgtaag agctcattgt gcggcttact
     4441 ggactgaact tgaggtttgt tttggcagac tttatgatgt caaatgtaat ctggaaaaca
     4501 tctgccttgg cttctttggc tttgcttggt gaaaatccca tataggtgcc aacttctccc
     4561 ttgattgtca aatcaaagaa tggggcgtat tcgggtcctt caagtaggga ttttgtttca
     4621 acaggtctta aaattgcttg atcatagatg atttttccgg caaaagattt catgaacttc
     4681 tggttctcat agccaactgt tacaaccaac tggttctttt tgcttgtatc aaacctcata
     4741 tatgacttca ttatgtcttc cggtttgaca acaatgcaga taccggtggc agaaaccctt
     4801 gcttcagaac tgctctgtga cattgctgta acaagatact cgtaagttgc aggttcggca
     4861 gaaggatcgg ccttaagatc aagcgttctt tcatcggtgg ccttcatcgt cgaggaaggc
     4921 atgaaatttc ttgcaagttt gcacctgcat gggaaaccgg agtttgaaag atagtataca
     4981 ttgctgtcgc cctcaatgct tccaaggcga attttgacag atttggattc tgtgggtgat
     5041 atgaaaacta ttgatgggtc aagccttaaa gagagcgatc cctgttttgg cctgacgcaa
     5101 acaacacggc ctgattttgt gccaacgaat atcgacccgc tactgtatga aggaggtgtg
     5161 gtgatgttgt ctttgaggtc aacgtctgca acaacagtcc catcaaaagc aaaaaatgcc
     5221 tggatgtggc cattttcggt ggccgcaaca aggttttgat ttatacctat gactgcctgc
     5281 ttgattgggc cagcagcttt ctggtcccag atggcattcc cgtcgccaag cctgatgcaa
     5341 aaaacttgtc catcttcagt gccaatacca acgtaggttg cattataata gcggcatggt
     5401 gcggcaacta tcgggcgctt tatatcataa gaccaatatt tct
//
LOCUS       DAOSRI010000105         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13783_length_5413_cov_4.067936, whole genome shotgun sequence.
ACCESSION   DAOSRI010000105 DAOSRI010000000
VERSION     DAOSRI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_5413_cov_4.067936"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="PK717_04100"
     CDS             complement(<1..1776)
                     /locus_tag="PK717_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR46876.1"
                     /translation="MSIKSVKIKGFKTFAEGVEIRLSHRLTAVVGPNGSGKSNLVDAV
                     RWGIGEHTARLLRISNPTDIIFAGFGARKPLGMAEVAISLDNREGKYPIEQSLVQIIR
                     RCYRDGENEYLINDTKCRLKDIEDLFRGTGLGKQTYSVVGQGEVEQVISATPAERLKV
                     LEELAGVDVLRTTKRSVESKLDQAKIQMEKISAHASESEYQYENLARQAEVLEKYQVL
                     TDRLGHLKLQLHLVDLRENVFESNSLESKSKVLELKRKETQAEIDKLEEVEDFSAKLD
                     ELEKERSAALATRESAIVNSTRATAQLEHMRASLATAQETLRNSTLEEESLKRREKQL
                     VYDLSQLEEEIEKRSVYLAKCEKELEETEKARPELTDFKKERQNIQTKLDQSKRQADD
                     ARSRLQEMQSKLVLFTERKRQAQSRTRELEETTFPQTQDDRNLDRELEETRISRSRLE
                     GQLASFVDNAQKIQGELMSVQAEKTQTAKEVEKLRKILDGMAFKPSKLGKPILSKTAD
                     LSGLSKEDADLARSQLDWIIASKAAAPGNGMSHIRGGHAHFDRANLVAAVDASLQRLQ
                     TDYIDLYIVHWQELPEFPCPIADTMG"
     gene            1904..3016
                     /locus_tag="PK717_04105"
     CDS             1904..3016
                     /locus_tag="PK717_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR46877.1"
                     /translation="MPIYLDNAATSFPKPKGVLDQVTDYMKNVGGVPTTGYACKPHAN
                     AMEIVNECRIALSRLITGSTPEQIYLCKSASEAISAIMRGVVEVGDKIAISDGDPWPI
                     QVPARELEQKGITVARLPFDHELFVNPKHLAKVAQKVRAVFVSHASNITGTLAPMGEI
                     SKVCQEADTMLVLDASHTVGVCPINLKHVKADAIIFSGHKGLYGPTGTAGVYVSPRLS
                     EKLTKLSKTNLSVHQILEVGTPNMAGLYGLLAGARFVDMEGVEKIWCHHKLLRESLVT
                     SLETVKKVKVVAPETWDGIGILSFSAGDLPINTMARLLEERYGLVVGNGLFANEGAMR
                     TLNLYPQGTLRASVGYFNTVSDMEYLATSLARIVGG"
     gene            3116..4186
                     /locus_tag="PK717_04110"
     CDS             3116..4186
                     /locus_tag="PK717_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46878.1"
                     /translation="MPSWKSPRGGFYNNSGTMLAPKQPLKVVWETKIPNQTHCNPVAA
                     GGMVVLSDDKGVVYCIDGENGNHVWEKQFYVGQINPVISKGRVLFGDQSGFFYSLNLA
                     DGRQVWSKTLSSPVVGWPTIDGDRVWVVTRDDLYCLEEAQGTEIFKKSYGVQLTQTPS
                     LQRYINLVASDTIIAVVPEDGKEVWRKKLEYPIIGPAVCVRDELLVTSGKLLKINALN
                     GEIVESYEFDTGSKDKKYKVDIVSPPSVYVSMVAIGLSNNMVVVVRQIDLKPVWGFGT
                     NKPVTSSAIITKESAFLAADDGKLYVIDVKNGNWQWHNNFKSPVVGAAILDTYLFALS
                     ESGILRRMDQGGEPLTQEEMNK"
     gene            complement(4190..>5413)
                     /locus_tag="PK717_04115"
     CDS             complement(4190..>5413)
                     /locus_tag="PK717_04115"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR46879.1"
                     /translation="LPKINLGETFVALRHRNYRLFFFGQLVSLIGTWMQNVAQSWLIY
                     DITNSKVYLGYVSFASSIPIFLVSLWAGVLLDRLPKRKVIIYTQVASMILAFTLAFDV
                     FFKTIQPWHIIVLSFLLGCVNAIDMPARQSFVIEMVDREDLMNAITLNSTIFNTARIV
                     GPSLAGLALAWLGAGWCFALNGFSFIAVIFALMSMKLEDRIAEPTRAHPFKQIVEGLV
                     YVKSNKTISMLILVIAVSNLFAFSYSALMPSFAKDVLNAGEVGFGLLQSFVGIGALMG
                     ALLMASVKSTSTNKGFMLTLGNIGFPLAALLFSLSKYLPISAFLLIFVGMGFMIQNAT
                     INTLIQLNSEDSKRGRVMSIYTLVFQGFFPVGSLLTTSFAEIVGTPIASITGEAIALV
                     FGVIWFLKVPFVRKL"
BASE COUNT         1375 a         1207 c         1302 g         1529 t
ORIGIN      
        1 tcccatcgta tcggcgatcg ggcaggggaa ctccggcagt tcctgccagt gcacgatgta
       61 gaggtcgatg tagtcggtct gcaggcgttg caggctggcg tccacggcgg ccactaggtt
      121 ggcgcggtcg aagtgggcgt gaccaccgcg aatatggctc atgccgttgc cgggcgcggc
      181 ggccttgctg gcgatgatcc agtccagctg agaccttgcg aggtccgcat cctcctttga
      241 aagccccgag agatctgctg tttttgagag aatgggtttt ccaagttttg atggtttgaa
      301 ggccatcccg tcaagtattt ttcttaattt ctcaacttct ttggctgtct gggttttttc
      361 agcctgcaca gacatcagtt caccctgtat tttttgggca ttatcaacga atgatgcaag
      421 ctggccctcc agccttgatc ttgaaattct tgtttcttca agctccctgt caaggtttct
      481 gtcatcttgc gtctgcggga aggttgtttc ttcaagctcc cttgttcttg attgtgcctg
      541 cctcttgcgt tcggtgaaca gtaccagttt tgattgcatc tcctgaagcc ttgatcttgc
      601 atcgtcggct tgcctttttg actggtcaag ttttgtctga atgttctggc gttccttttt
      661 aaaatctgtc agttctggcc tggctttttc ggtctcttcc agttcttttt cgcattttgc
      721 gaggtagaca gagcgtttct caatttcctc ctcaagttgt gaaaggtcat aaacgagctg
      781 tttttcacgc cttttcagcg attcttcttc gagggtggaa ttgcgcaatg tctcctgcgc
      841 tgtggcaagg cttgccctca tatgttccag ctgggctgtt gccctggttg aattgacgat
      901 ggcactttcc cttgttgcca gcgccgcaga cctttccttc tcgagttcat ctagttttgc
      961 agaaaaatcc tcaacttcct caagtttgtc tatctcggcc tgggtctcct tgcgtttcag
     1021 ctccagcacc tttgatttag actcaagcga gtttgattca aagacatttt ctctaaggtc
     1081 gacaagatgg agctggagtt tgaggtggcc cagcctgtct gttagaacct ggtatttttc
     1141 cagcacctcc gcctggcggg caaggttttc gtattggtac tccgattcgc ttgcatgggc
     1201 agagattttc tccatctgga ttttggcctg gtccagtttt gattcgacag agcgttttgt
     1261 cgtcctgagg acatcaactc cggcgagttc ctcgagcact ttcagccttt cggcaggtgt
     1321 tgctgaaatt acctgctcga cttctccctg gccaacgacc gagtaggtct gttttcccag
     1381 tccggttccc ctgaagaggt cttcaatgtc tttcagcctg cattttgtgt catttatcag
     1441 atactcgttt tccccgtccc tatagcacct tctgattatc tggacgagtg attgttcaat
     1501 cggatatttt ccttccctgt tatcaagcga tattgcaact tcggccatac cgaggggctt
     1561 tcttgctccg aaaccagcaa aaattatgtc ggtaggattt gatatcctca acagccttgc
     1621 cgtgtgctct cctatccccc accttacagc gtcgacaagg ttgcttttgc cggagccatt
     1681 tgggccaacc acggcggtca atctgtggga aagccttatt tcaacgcctt cggcaaaggt
     1741 cttgaatcct tttattttta ccgacttaat ggacatcaga aagcatttta gggcctggcc
     1801 gcttttcaat caatgcttga ttaggtttgg cagttttggt acgatctagt cgtgtttaga
     1861 acaaacattt tcactgttca ttctgtcctc aaggagggga gcagtgccaa tttaccttga
     1921 caatgcagcc accagctttc ccaagccaaa gggagtactg gatcaggtaa ccgattacat
     1981 gaaaaatgtt ggtggtgtac caacgacagg ctatgcctgc aagccacatg ccaacgcaat
     2041 ggaaattgta aatgaatgca gaatagcact ttccaggctt atcactggct cgaccccgga
     2101 acagatatat ctttgcaaat cggcaagtga ggccatttct gcaattatgc gcggtgttgt
     2161 ggaagttggt gacaagattg ccatcagtga tggtgatccg tggcctattc aagtcccggc
     2221 aagagaactg gagcagaagg gtataactgt cgccaggctt ccctttgatc atgagctttt
     2281 tgttaatccc aaacatctcg caaaggttgc ccagaaagta agggccgttt ttgtttccca
     2341 tgcttcaaac atcactggca cccttgcacc gatgggtgag atatcaaaag tatgccagga
     2401 agctgataca atgcttgttc ttgatgcttc ccacacagta ggcgtttgtc caataaacct
     2461 gaaacatgta aaagcagatg caataatttt ctcaggccac aagggtcttt atggcccgac
     2521 aggaactgcg ggtgtttatg tttccccaag gctctcggaa aaattgacaa aactctcgaa
     2581 gaccaacctt tctgtccatc aaatattgga agttggaaca cccaacatgg ctggattgta
     2641 tgggctactg gcaggagcga gatttgttga tatggaaggt gttgagaaga tatggtgtca
     2701 ccataaatta ttgagagaaa gtttggtgac ttcgcttgag acagttaaaa aagtgaaggt
     2761 ggttgcgcct gaaacgtggg atggaattgg tatattgtct ttttctgcag gcgaccttcc
     2821 aatcaacacc atggccaggc tgttagaaga aagatacggg ctggtggttg gaaatggtct
     2881 ctttgcaaat gaaggtgcca tgaggacact caatctttac ccgcagggca cgctcagggc
     2941 atcggttggc tatttcaata cggtatccga tatggagtat cttgccacct cacttgccag
     3001 aatagttgga ggttgatccg tgaaatccat aaaaattata tctatggcca cattattagt
     3061 aacactggtt ttattttgtg gctgccagag cgctacgtta attgatactg agacaatgcc
     3121 aagctggaag tcaccaagag gtggcttcta caataactcc ggtacgatgc ttgccccaaa
     3181 gcaaccactc aaggttgttt gggaaacgaa aatccccaac cagacacatt gcaatccggt
     3241 agccgcaggt ggaatggttg ttctctcaga tgacaagggt gttgtctact gcatcgatgg
     3301 tgaaaatgga aaccatgtct gggaaaaaca attttatgtt ggccagataa atcctgtaat
     3361 ttccaaagga agggttttgt ttggtgacca aagtggtttt ttctactcat taaaccttgc
     3421 agatggtaga caggtctggt caaaaacact ttcttcacca gttgttggct ggccaaccat
     3481 tgatggtgat agggtttggg ttgtaaccag agatgatctg tattgccttg aagaggccca
     3541 gggaaccgaa atcttcaaaa aaagctatgg agttcagctg acgcaaaccc cttcgctcca
     3601 gagatatatc aaccttgttg catctgacac aatcattgcg gttgttcccg aggatggtaa
     3661 ggaagtatgg cgtaagaagc tggaatatcc aataattggt cctgctgtgt gtgtgagaga
     3721 cgaattgtta gtgaccagcg gaaaacttct taaaataaat gccctcaatg gtgaaattgt
     3781 agaatcttat gaatttgata caggttcgaa agacaaaaaa tataaggtgg acatagtctc
     3841 ccctccatct gtatatgtaa gcatggtggc aattggactg agcaacaaca tggttgttgt
     3901 tgttaggcag atagatctta aaccagtctg gggctttggc acgaacaagc cagtgacatc
     3961 aagtgcaata attactaagg agtcagcttt tctcgcggca gatgatggca agttgtatgt
     4021 cattgatgtg aagaatggca actggcagtg gcacaataac ttcaaatcac cagttgttgg
     4081 tgcggcgata ttagacactt acctgtttgc tttgtcagaa agtggcattt tgcgtcgcat
     4141 ggatcagggt ggcgagccac tcacccagga agagatgaac aagtaagaat caaagcttcc
     4201 tgacaaaagg cactttcagg aaccatataa ccccgaaaac taatgctatg gcctcgcctg
     4261 ttattgaggc aatcggtgtt ccaactattt cagcaaatga tgttgtaagc aatgagccca
     4321 ccgggaagaa cccttggaaa acaagcgtat aaatgctcat tacccttccc ctcttggagt
     4381 cttctgagtt tagttggatg agggtgttga tagttgcatt ctgtatcata aagcccatgc
     4441 caacaaatat cagaagaaaa gctgaaatgg gaaggtattt tgacaacgaa aacaggagtg
     4501 cggccagcgg gaaaccaata ttgccaagcg taagcataaa acctttgttt gtgcttgtgg
     4561 attttacgga tgccatcaat agcgcaccca tcaatgcccc aatgcccaca aagctttgta
     4621 gaagcccaaa ccccacttcg ccagcgttaa gaacatcttt tgcgaaagaa ggcatcagag
     4681 ctgagtagga gaaagcaaac aggttggaca ctgctatgac caggataagc atgcttatag
     4741 tcttgtttga ttttacatag acaagccctt caactatctg tttaaatgga tgtgcccttg
     4801 ttggttcggc gatgcgatct tcaagcttca tggacatgag tgcaaaaatg acagcgatga
     4861 agctaaaacc attcaacgca aagcaccacc cagcacctag ccaggccaga gccaaaccag
     4921 ccaatgatgg gccaacaatc cttgcggtat tgaatatcgt tgagttgagg gtgatagcgt
     4981 tcataaggtc ttctctgtcg accatttcta ttacaaatga ctgtcttgct ggcatgtcta
     5041 tggcgttgac acatccaaga agaaaagaca agacaataat gtgccacggc tggattgttt
     5101 tgaaaaacac gtcaaacgcc agggtgaacg ccagaatcat tgatgcaacc tgggtgtata
     5161 tgattacctt gcgtttaggg agcctgtcca ggagaacacc tgcccaaaga gacaccagaa
     5221 atattggaat ggatgaagca aaacttacgt atccaagata cacctttgaa tttgtaatgt
     5281 catatatgag ccagctttgg gcaacgtttt gcatccatgt tccgataagt gatacaagtt
     5341 gtccaaagaa aaaaagcctg tagtttctgt gtctaagtgc tacaaatgtt tcaccgagat
     5401 tgattttagg gag
//
LOCUS       DAOSRI010000106         5411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13787_length_5411_cov_3.921583, whole genome shotgun sequence.
ACCESSION   DAOSRI010000106 DAOSRI010000000
VERSION     DAOSRI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13787_length_5411_cov_3.921583"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..687)
                     /locus_tag="PK717_04120"
     CDS             complement(52..687)
                     /locus_tag="PK717_04120"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOR46880.1"
                     /translation="MGIWKELLDFVFPPSCVLCGENGTHPLCRSCMGKIEIDPVCSTK
                     MGLTVTSFSKYEDATRTILARFKYDFQEQVGVIIADLMTPYLSTLAPRILVPVPLHFV
                     KIRQRGFNQSAIIAKRLSKNLGWKYTPRLIIRQRNTKPQFELDDEERLANVEKAFARY
                     PFATIHPDHPYMLVDDIVTTGATLASCAKMLRESGATRIEGLTFARARNVN"
     gene            complement(662..1729)
                     /locus_tag="PK717_04125"
     CDS             complement(662..1729)
                     /locus_tag="PK717_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOR46881.1"
                     /translation="MKKLTLWGYYGFGNVGDEALLDVCLSLMKDVKVDLVCGPMPSVK
                     VSEKLSIVPRSPENLYKSIKSSDGFVLGAGGLIHERAKPLGTYYHLLGPFISKYSRKP
                     YCAIGQQIGPFQRDLTKTLAKKALEKARFITVRDNISQVEASFLGLNTTLSADLAFLL
                     NPEDPSSEIKKVIESMPKPRIIFAPASYMEETPSPEFCAKVLGRIISLTGGSLIIIPF
                     FPGKDDEFGEKTETLLLPGRVLRLKSLIDWRDAFGAFNLCDFSVPMRLHSVIASSLAC
                     IPALPMPYFQKVRSISSEIGYSTMIEKDDPDWEQKCLSFLSTSQNICQQTALLAKDMR
                     KRAEITAKLMEGFIGDMERTA"
     gene            complement(1726..2448)
                     /locus_tag="PK717_04130"
     CDS             complement(1726..2448)
                     /locus_tag="PK717_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOR46882.1"
                     /translation="MNETGNFIRGLRWHIGDLDGTADRIANLLNDNKINHIITTNMEI
                     LSMAFKNKELRELCTNADMHIPDGIGAVRLLKKLGFSKTQRIPGIELCLEALKKMPEG
                     TKIYLFGAREGVAIKALEKLSARFLQLTFAGWHEGFGYEGNKLVEEINNSGAQVLLVA
                     LGVPKQELWIAKNKCNLNNIRVAIGVGGSFDVWAGDVNRAPRFFQKTNLEWAWRILSQ
                     PARLPKFLKTLAGFLPIYLRAK"
     gene            complement(2445..4751)
                     /locus_tag="PK717_04135"
     CDS             complement(2445..4751)
                     /locus_tag="PK717_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOR46883.1"
                     /translation="MIGIRDDGSHFKYPKSVFVDPESKNMFVADTGNKRIVLLKSDDG
                     TFIMDFGKERLKNPTKVIQDKGSRLLVCDPEAKAIHLFDRKGAYLADFSVGNDPVTGI
                     IEPISLAFDKNGRFFVLDHGACQIKIFESSGEFLFSMGSKGDGESQLLEPSDVALDAE
                     GNVYATDTGNNRVVVFDKTGTFKISFGQDELSHPTGIAINSTNVYVSNTGKHEFSVFS
                     KKDFTYKGNYGAIGSGNSQLRFPIGLCLDSDQKLWIADSGNCRVVQTQKLEFVKSIGT
                     DGITISPKGLVLKDGMIYVSEPEANCINVYEVQSGSFSRSIGLQGTNLTSLNSPQSCC
                     ISSRDDLIVADTGNHRIQVYSLSGEHLVSFGSQGSGIGQFESPTSIACTKSGNIIVAD
                     SGNSRVCVLTLGGAWQFDITKELKKPVAVAEDMIGNIWVCDQELGKVLIFDQKGNQVN
                     EIQGFNKPSAIFCDAMGRLFVADEETGLIKVFSTTGYELAAIGGEGGPMSATKPPFLP
                     EQPGKFFKPYGITASDDYVYVSDTGNSRIQKIPLAAFGGLPKLGTDKFTLDFPKVPRS
                     SKRSQTLKITNDGDGLIEGTISTDQPWILVGQATFTGDTEVTITLDGTKAPEGQREGT
                     LTIQSNGGIVKIKITGRFFTGQIKRLEIKVGSNTVISSGKKIYVTPAPLKDTKYNKVY
                     TAFRFVGEELGADVILSGKKIIYILEGRTLQLTIGSQKASFQGKEMDMGSPCYMYGKV
                     PLVPLRFVAETLGATLMSEGNKIIVEYP"
     gene            complement(4811..5362)
                     /locus_tag="PK717_04140"
     CDS             complement(4811..5362)
                     /locus_tag="PK717_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR46884.1"
                     /translation="MELPANLRETTLAELIQLIELHSASGRLQIIKTDTDERASIFFT
                     QGQVVHATTPRCQGEDAIIELFSWDKGKILFSEASAIPPRSINLSNTDIIIKGLGEAQ
                     ETAEFMIGLPPMPTVLRLNTSSMSGDGGEKITLDISEWNFLILVDGRRTLQQIFDECN
                     LSQSQAAKLIGRLLEQKVVVPIR"
BASE COUNT         1308 a         1218 c         1182 g         1703 t
ORIGIN      
        1 ttcctccatg gcggttttat ttatcacaaa ttcctcgcgt ttcaatggtt gctagtttac
       61 attcctcgcc ctggcaaaag taaggccttc gatccttgtt gcacctgact ctcttaacat
      121 ttttgcacag cttgccagtg ttgctccggt ggtgacgatg tcatcaacaa gcatgtatgg
      181 gtggtccgga tgtatggttg caaaaggata tctggcaaaa gctttttcta catttgccag
      241 ccgctcttca tcatcgagct caaactgggg ttttgtgttc ctttgcctga tgatgagcct
      301 tggggtatat ttccagccga gattttttga aagtcttttt gcaatgatcg cagactggtt
      361 aaatcctctc tggcgtatct tgacgaaatg caatggtacc gggacgagta ttcttggcgc
      421 gagagtcgat aagtacgggg tcatgaggtc tgcgatgatt acccctactt gttcctgaaa
      481 gtcatacttg aaccttgcca gtattgttct tgttgcatcc tcgtattttg aaaaactggt
      541 gaccgtcaac cccattttgg tcgaacaaac gggatctatt tctatttttc ccatgcaact
      601 gcggcacagg gggtgggtgc cgttttcccc gcacagcaca catgatggag ggaatacgaa
      661 atcaagcagt tctttccata tccccaatga agccctccat taactttgcg gttatctcgg
      721 cacgcttcct catatctttt gcgagaagag ctgtttgctg gcatatattt tggcttgtgg
      781 agaggaagga caggcatttt tgctcccaat ctggatcatc cttttcaatc atggtgctat
      841 agcctatctc ggaggatatg gaacgtactt tttgaaagta tggcatgggc aaagctggaa
      901 tacaggccag tgaggatgct ataacagagt gaagtctcat tgggaccgaa aaatcgcata
      961 agttgaatgc cccaaaggca tctcgccagt caataagact tttgagcctc agtacccttc
     1021 cagggagcaa taacgtctct gtcttttcgc caaattcgtc atctttgccc gggaaaaacg
     1081 ggattattat taaagagcca ccggttaatg agatgatcct tccaagaact tttgcgcaga
     1141 attctggaga aggggtttcc tccatgtaag aagctggtgc aaaaattatt cttggttttg
     1201 gcattgattc gattactttt ttaatttcgc ttgatgggtc ttcggggttc aggagaaatg
     1261 ccagatctgc agaaagtgtt gtgttcagac caagaaagct tgcttcaact tgcgagatat
     1321 tgtctctaac ggttataaat cttgcctttt caagcgcttt ttttgcaagt gtttttgtaa
     1381 ggtccctctg aaaagggccg atttgctggc caatggcgca gtacggtttc ctggaatact
     1441 ttgatatgaa tggccccaaa aggtggtagt aggttccgag tggttttgct ctctcgtgta
     1501 tcaagccccc ggcgccaagc acaaaaccat cagaagattt tattgatttg tacagatttt
     1561 ccgggctcct cgggacaatg gacagctttt ctgaaacttt aaccgatggc ataggcccac
     1621 atacgaggtc aactttgaca tctttcatga gactcaggca aacatcaaga agcgcctcat
     1681 cgccaacatt tccaaatcca tagtagcccc agagggtgag tttcttcatt ttgccctcaa
     1741 atagatcgga agaaaaccgg caagggtctt caaaaacttt ggtaatcttg caggttgcga
     1801 taaaatgcgc caggcccatt ccagatttgt tttctggaaa aacctgggtg ccctgtttac
     1861 atctccggcc cagacatcaa agcttccgcc gacccctatt gcaacacgaa tattatttag
     1921 attacacttg ttttttgcta tccacagttc ctgctttggg acgcccaggg ccacaaggag
     1981 aacttgtgcc ccggaattgt ttatctcttc caccaatttg ttgccttcat atccgaaacc
     2041 ctcatgccaa ccagcaaagg tcagttggag gaacctggca gaaagttttt caagggcttt
     2101 tatggcaaca ccttcccttg ccccaaacag gtaaatcttt gttccttcgg gcattttttt
     2161 gagtgcctca aggcagagct ctatgccagg gattctttgt gtttttgaaa aaccaagctt
     2221 tttaagcaac cttacggcgc caatgccatc cggaatgtgc atatcggcat ttgtacacaa
     2281 ttcccggagt tctttatttt tgaaagccat ggaaagaatc tccatatttg ttgtgattat
     2341 atgattgatt ttgttgtcat ttagaaggtt tgcgattcta tctgcggtgc catcaagatc
     2401 gccgatgtgc catctgagtc cccttataaa attgccagtt tcgttcatgg gtattcgaca
     2461 attattttgt tgccctcgga catcaaggtt gcgccaagtg tttcggcaac aaacctgagg
     2521 ggcacaagtg gtactttgcc atacatgtag catggtgaac ccatgtccat ttcttttccc
     2581 tggaatgatg ctttttggga ccctattgtt agctgtaatg ttctgccttc caggatatag
     2641 attatttttt taccggagag tattacgtct gccccaagct cttcgcctac aaatctaaaa
     2701 gcagtgtaaa ccttgttgta tttagtatct tttagtggtg ccggcgtgac ataaatcttt
     2761 tttccgctgg agatgactgt gttgctgcca acctttattt caagccgttt gatctgtccg
     2821 gtaaaaaatc ttccagtgat tttgattttt actatgccac cattgctttg aatggtgagg
     2881 gttccttctc tttggccttc tggcgctttt gtcccatcaa gtgttattgt tacctcagtg
     2941 tcgccggtga atgttgcctg tccgactagt atccatggct ggtcggttga aattgttcct
     3001 tcgatgagcc cgtcgccatc attggttatt tttagtgttt gggaacgttt tgacgatctt
     3061 ggcacctttg gaaaatccaa agtaaatttg tctgttccga gttttgggag tccgccaaat
     3121 gccgcaagag gaatcttttg gatgcgggaa ttccctgtgt ctgaaacata aacgtaatca
     3181 tcagaagctg ttattccata aggcttgaag aacttgccag gttgttctgg caagaaaggt
     3241 ggttttgtag ctgacattgg gccgccttcc ccacctattg ctgcaagttc gtaccctgtt
     3301 gttgaaaaca cttttataag acctgtttct tcgtcagcta caaacaatct tcccattgca
     3361 tcgcagaaaa tcgcacttgg tttgttgaaa ccttggattt cattgacctg gtttcccttc
     3421 tggtcaaata ttagcacctt gccaagttcc tggtcgcaca cccagatgtt gccaatcatg
     3481 tcctctgcaa ctgcaacagg tttttttagc tcttttgtta tgtcaaattg ccaggcaccg
     3541 ccaagtgtga gcacacaaac ccttgagttg cccgagtcgg ccacgattat gtttccgctt
     3601 tttgtgcaag ctatggatgt tggtgattcg aactgaccga ttccagaacc ttgcgagcca
     3661 aaactcacaa ggtgttcccc agacagactg tatacttgta tcctgtggtt ccctgtgtct
     3721 gcaactataa gatcatctct tgacgatata cagcatgatt gtggtgaatt taggcttgta
     3781 aggtttgttc cctgaaggcc tatggatctt gaaaaggacc cactttggac ttcgtaaacg
     3841 ttgatgcagt ttgcttctgg ctccgagaca taaatcatgc catctttgag aaccaaacct
     3901 tttggggaaa tcgttatgcc atcagtaccg atggatttta caaattccag tttttgagtt
     3961 tgaacaaccc tgcagttgcc ggaatcggct atccagagct tttgatcgga atcaaggcaa
     4021 agtcctattg ggaacctaag ctgggaatta ccacttccga ttgccccgta attccccttg
     4081 taggtaaaat ctttttttga gaagacagag aattcgtgct tgccagtgtt tgagacgtat
     4141 acattggtgg aatttattgc tatccctgtt gggtgggaga gttcatcttg gccaaaagaa
     4201 attttgaagg tgccagtttt atcaaatacc acaaccctgt tgttgcctgt gtcagttgca
     4261 taaacattcc cttctgcatc gagagcaaca tcagaaggtt ctaataattg gctttcccca
     4321 tcacctttgg accccatcga aaacagaaat tcacccgagc tttcaaatat cttgatttgg
     4381 caggccccat gatcgagcac aaagaatcta ccatttttat caaaagccaa tgatattggc
     4441 tcgataatgc ctgttacagg atcattccct acggagaaat cggcaaggta agcccctttt
     4501 ctgtcgaaaa gatggattgc ttttgcttct gggtcacaaa ctagcagtct tgaaccctta
     4561 tcctggatca cttttgttgg atttttcaat ctttctttgc caaaatccat tatgaatgtg
     4621 ccatcatcac tttttaatag cactattctt ttatttccgg tgtctgccac gaacatgttc
     4681 tttgactcag ggtcaacaaa tactgatttt gggtatttga aatgtgaacc atcgtctcta
     4741 atgcctatca tccccacagg aaacgatgag gtagaaataa tggcaataac tgacagaatt
     4801 gaaaaaaatc tcatcttatg ggaacaacca ccttttgctc aagaagtctt ccaattaatt
     4861 ttgccgcctg gctttggcta agattgcatt catcaaaaat ttgctggaga gttcttctgc
     4921 catcaaccag tattaaaaaa ttccattccg atatatctag cgttatcttt tcgccaccat
     4981 ctccactcat cgagctggta ttgagtctta atacggtggg cattggaggg agccctatca
     5041 tgaattccgc agtttcctgc gcttcaccaa gtccttttat tatgatatca gtgtttgaga
     5101 ggtttattga tcttggtggg atggcagatg cttccgaaaa taatattttc cctttgtccc
     5161 aagagaagag ttcgattatt gcgtcttctc cctgacatct tggggttgtt gcgtgaacaa
     5221 cttgcccctg cgtaaagaaa atggaggctc tttcgtcggt gtcggttttt attatctgga
     5281 gccttcccga ggcgctgtga agctcgatca gttggataag ctccgcaagt gtcgtttccc
     5341 tgagattggc tgggagttcc actgtcagct ccagtccgct tgggtcttat tcaggaaagc
     5401 ttccatacct t
//
LOCUS       DAOSRI010000107         5411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13789_length_5411_cov_3.544249, whole genome shotgun sequence.
ACCESSION   DAOSRI010000107 DAOSRI010000000
VERSION     DAOSRI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_5411_cov_3.544249"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK717_04145"
     CDS             <1..956
                     /locus_tag="PK717_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOR46885.1"
                     /translation="ISLVCLFMGTAMAAIAAYYDGLAWDRSILHGSWMFMSAWSTGGF
                     GPCTQNLLFYHSYAFEIILCIFMMMGSINFSLHYIVWKGKPKELLKDIEIKSLVVTLS
                     ITTMLVAWGLSSTGIFTNLNAVLRRGIVLVVSGHTGTGQMTVYAKQLVHSWHPLAYMG
                     ICIAMMLGGSAASTAGSFKALRIGIFFKGLIHETKRLILPESAVFKMTFHHIKDQIIE
                     SGMVKMAGLIILCYCFIYLFGAVVGVACGYPLSQATFESISAGANVGLTMGVVSPSMP
                     AILKITYIITMWLGRLEFLSIFVLIGSVGLAIGQAFKGGRK"
     gene            953..1504
                     /locus_tag="PK717_04150"
     CDS             953..1504
                     /locus_tag="PK717_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46886.1"
                     /translation="MRHYIAILTLAIIVLVPQIAFASSPVYSPTDLITKALELDGKTI
                     TVRGEAITEALERNNGYSWVNIFDGSSAIGIWMKNDLKDQIKNYGTNLVKGDIVEITG
                     KFFRNDPEADGELDLRADSLKIVESGYPTPEKINPLKVIILVILSLVALLLFADNIYK
                     NKKKTQEKKEDFEIENAIHEELE"
     gene            complement(1506..1706)
                     /locus_tag="PK717_04155"
     CDS             complement(1506..1706)
                     /locus_tag="PK717_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46887.1"
                     /translation="MMILWILPLIIVPIAVALAFVLWYGRRNPKEEPENKEIFENVER
                     ALKEGKITEEDAERIRKSIEEE"
     gene            complement(1776..2246)
                     /locus_tag="PK717_04160"
     CDS             complement(1776..2246)
                     /locus_tag="PK717_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46888.1"
                     /translation="MDVDTMKEEFMSKGTTDIHIKKGTIHIIKALILGFGLMLISRLF
                     SVFGSYVGTAFSALFMTALTWGGLMYCWKDISKGWDKGAMVGILYGVVFFILDLIFGL
                     LLHFGFTPFGHAMAGVGLGIGNDIWLVILTIFYIIGLGVFGAVFAWIKDQMNVA"
     gene            complement(2397..2780)
                     /locus_tag="PK717_04165"
     CDS             complement(2397..2780)
                     /locus_tag="PK717_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46889.1"
                     /translation="MKFDVKHIIYALLISIGWIVLSWIFAQIPGIGEFLNKLLMFIWI
                     YGLLVYAMKDVKGLADGVVTGLIYAIIFAIVTIIFSYINWGGTFPFMKFAGDYGAFFG
                     WPGWWGDLVGIALFGGMLGWINEQK"
     gene            3002..4579
                     /locus_tag="PK717_04170"
     CDS             3002..4579
                     /locus_tag="PK717_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46890.1"
                     /translation="MKKISTAILLTAIFLAGCVAPGQETPVKPATSPKIATNDFFPLG
                     WFTSLSKDEIGGLKDVGANFAITYVLSGRLKKELLSDVLDEAERKNIKVLLDVTSDWK
                     WFDDDMPIPNDPIGDWNWSLDHAYSKRASHHGGWNDELNNHGFVMPEAGYPAMNVQDH
                     DMIEQWVYIPSKNDVKELMIEFYASKSGPSFSWNHRVYWGEDLIKRGDPKDKEIVSPF
                     RAGDLPKERDKWVKLTFRANDINLDATLVRGVNFINYGSQVYWDLMTRRTGKERIQER
                     VNYLKGKKALFGWYLCDEPELRNIDPPRLKEIYDQIKEVDQEHIIAPVFQDWKVMEQY
                     RDSCDIFFLDCYPVTVKSRSMDWMTQWVVEGARVAAKYKKPFVFVPQGFGDAPEYPLW
                     TMPTKHELRWMTWTPIILGARGVCYWAYYKASKEIVEESGKLFKEIGLYKDFLTKGTP
                     VTGLSSSITSDSDFDGNPDLMYSAYDFAGRRLLMVSNGTSRRFDNVKLVGPGYEKDFD
                     LEPYGVKIIMENLEDNR"
     gene            4576..4761
                     /locus_tag="PK717_04175"
     CDS             4576..4761
                     /locus_tag="PK717_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46891.1"
                     /translation="MTRWTLIVASLVMMAGGILMMVIKDINGHPIGVPSQIAPVVGWI
                     VIILGFLFLMLSMIRSE"
     gene            4754..5290
                     /locus_tag="PK717_04180"
     CDS             4754..5290
                     /locus_tag="PK717_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOR46892.1"
                     /translation="MSSDKSEEKNLVERAKKGEESAFTEIVELYADKVFSEAYYIMNN
                     REDALDASQLVFLRVFQNIKHFKGESLLSTWIYRITVNTCLRELKKRNRKPPEPDDDD
                     PDPQDEVIRNEEEKIAMEILAKLPEPYRVILIMREMNDMPFKDISDVLGISVNLAKVR
                     AFRAKARFKQLLEEQENG"
BASE COUNT         1635 a         1157 c         1194 g         1425 t
ORIGIN      
        1 taatatcact tgtttgcctt tttatgggaa cagctatggc agcaattgct gcttactatg
       61 atggtcttgc atgggacaga agcatccttc atggttcatg gatgttcatg tcggcttgga
      121 gcaccggcgg tttcgggcct tgtacccaaa atttgctctt ttatcattcg tatgcttttg
      181 aaattatctt gtgtatcttc atgatgatgg gatcaatcaa tttctcattg cattatattg
      241 tctggaaagg caaacccaag gaactcctca aagatataga aatcaaatca ttggtcgtta
      301 ctttgtcgat tactaccatg cttgttgcct ggggactctc atccacgggc atttttacaa
      361 accttaatgc agtattgaga agaggaatag tgcttgttgt ctctggtcat acgggtaccg
      421 gccaaatgac ggtatacgca aaacagttgg ttcatagctg gcatcctctt gcctatatgg
      481 ggatatgtat agctatgatg cttggcgggt ccgcggcatc aactgctggt agtttcaagg
      541 cccttcgaat cgggattttt ttcaaaggat tgatccatga aacaaagaga ttgattctgc
      601 cagaatcagc agttttcaag atgacttttc accatatcaa agatcagata attgaatctg
      661 ggatggtgaa aatggcaggt ttgattattt tgtgctattg ttttatctat cttttcggcg
      721 cagtggttgg agtggcttgt ggctatccgt taagtcaggc aacttttgag tccatatctg
      781 ccggagcaaa tgtcggattg acaatgggtg tcgtatcacc atccatgccg gccattttga
      841 aaataaccta cattataacg atgtggcttg gcaggcttga gttcctgtcc atctttgtac
      901 tcattggaag cgtcgggctg gcgattggcc aggctttcaa ggggggtaga aaatgaggca
      961 ctacatcgcg atattgactc tggcaatcat tgttctggtg ccgcaaatag ctttcgcttc
     1021 ttcaccagtc tattccccaa cggatctgat cacaaaagcc cttgagcttg atggaaaaac
     1081 catcaccgtg cgtggagaag caatcaccga ggccctggaa cgcaataatg gctactcatg
     1141 ggtaaatata tttgacggtt cttctgcgat aggcatctgg atgaaaaatg acctaaagga
     1201 ccagataaaa aattatggga caaatcttgt caaaggcgat atcgtagaga ttacaggcaa
     1261 atttttcaga aacgatccag aggcagatgg cgagcttgat ctaagggcag attcactgaa
     1321 gattgtggaa agtggctatc ctacgccaga aaaaataaat ccattaaaag ttatcattct
     1381 ggtgatattg tctcttgttg ccctgcttct ttttgccgac aacatataca aaaacaagaa
     1441 aaaaacacag gagaaaaaag aagatttcga gatagaaaat gctatccacg aagaactgga
     1501 ataaatcatt cttcttcgat tgatttcctg attctctcgg cgtcctcttc ggttatcttt
     1561 ccttctttta gcgctctctc aacattttca aatatttcct tgttctctgg ttcttctttt
     1621 gggtttctcc tgccatacca gagaacaaat gcgagtgcta cagcaattgg cacgattatc
     1681 aacggtaata tccagagtat catcatgatc aaattaaaac gggccctgac cttgtggtca
     1741 agggcccgtt ggaaattatt tcttctactc ttgaatcagg caacattcat ctggtctttt
     1801 atccaggcaa acacggcccc aaaaacacca aggccaatga tatagaaaat ggtaaggata
     1861 accagccata tatcatttcc aatgccgaga cccactcctg ccattgcatg tccgaacggc
     1921 gtaaaaccaa aatgaagaag aagtccaaaa atcaaatcaa gtataaaaaa caccacacca
     1981 tacaagattc ccaccatggc gcccttgtcc cagcctttgc ttatgtcttt ccagcaatac
     2041 ataagcccac cccaagtcag agcggtcata aacagggcag aaaaagccgt accaacatag
     2101 gacccaaaaa cactaaacaa tctcgaaata agcatcagtc caaaaccaag tatcagggcc
     2161 tttatgatat gaatggtacc cttttttata tgaatgtctg ttgtaccctt gctcataaac
     2221 tcttctttca ttgtatcgac gtccataaat tcctccttat tcggtttgta ttgttaaata
     2281 atatgggaaa taaccctgaa cataatttac aaagaattta caagtatggg caatttgata
     2341 aaaaaatggg tcgccatttt ttggcgaccc attttgtgct tctaggatat ttcgaattac
     2401 ttctgctcgt ttatccagcc aagcattccg ccgaagagtg cgataccaac gagatcgccc
     2461 caccatcctg gccagccaaa gaacgcgcca taatcgccag caaatttcat gaatgggaag
     2521 gtcccgcccc agttaatgta agagaagatt atcgttacga tagcaaaaat tatcgcatag
     2581 ataaggccag tgacaacacc atctgcaagt cccttaacat ccttcattgc atacacaagg
     2641 agaccataaa tccagatgaa catgaggagt ttgtttaaaa attcgccaat ccccggaatc
     2701 tgtgcaaaaa tccagctcaa aacaatccac ccgatagaga tcaacagtgc ataaataatg
     2761 tgctttacgt caaatttcat ttcaataaac ctcctcttat ttttatttca gagctttcta
     2821 ccctgatacc acctttgatg ttcttaactt tcaaaaggtt acacttaatg atcacgttgc
     2881 caaaaaatac ttgaaatgat atttggccat tgggccccca aagaggactc atttcaataa
     2941 aagatattaa ccaaccttgt ttgagaaggt catttaatgt tatataatta tccgggctca
     3001 tatgaaaaaa atctcgacag cgatactact tacagcaatc tttcttgcag gctgtgttgc
     3061 tccaggccag gaaacacccg taaaaccggc aacaagccca aaaatcgcca ccaatgattt
     3121 cttcccgctt ggttggttca cctctcttag caaagatgaa attggcggcc ttaaagatgt
     3181 tggggccaat ttcgccatta cctatgtcct ctctggaagg ctaaaaaaag aactgctctc
     3241 tgatgtactt gatgaggccg agagaaaaaa tataaaagtg ctcctggatg tcacatcgga
     3301 ctggaagtgg tttgatgatg acatgccaat acctaacgat cctattggcg actggaactg
     3361 gagcctggac cacgcctact cgaaaagggc aagccaccac ggcggatgga acgacgaact
     3421 gaacaatcat ggtttcgtca tgcccgaagc tggttacccg gcaatgaatg tccaggacca
     3481 cgacatgata gaacagtggg tctatatacc atcaaaaaat gacgtgaaag aattgatgat
     3541 cgagttttac gcttcaaaga gtggtccatc attcagctgg aaccacaggg tatattgggg
     3601 cgaagatctc ataaaaaggg gcgacccaaa agataaagaa attgtcagcc ctttcagggc
     3661 cggcgatctc ccaaaagaaa gagataagtg ggtaaagctg actttcaggg ccaatgacat
     3721 caatcttgac gccacgctag ttcgcggcgt caatttcatc aattacggct cacaggtcta
     3781 ttgggacctg atgaccagaa ggaccggcaa agagcgcata caagagaggg tcaactatct
     3841 aaaaggcaaa aaagcccttt ttggctggta tctttgcgat gagccagagc tgaggaatat
     3901 tgacccacca aggctcaaag agatttatga ccagataaag gaagttgatc aggagcatat
     3961 aattgcccct gtctttcagg attggaaggt catggagcaa tacagggact catgtgatat
     4021 cttcttcctg gattgttacc cggtgacagt aaaaagcagg agcatggact ggatgaccca
     4081 atgggtagtg gaaggtgcaa gggtcgctgc aaaatataaa aaaccgtttg ttttcgtgcc
     4141 tcaggggttt ggtgatgcgc cagaataccc actatggaca atgccaacaa aacacgagct
     4201 caggtggatg acctggacgc ccattatcct tggcgcaaga ggcgtctgct actgggccta
     4261 ttacaaagca tcgaaagaaa tcgttgaaga atcgggaaag cttttcaaag agattggttt
     4321 gtacaaggat tttttgacaa aaggcacacc agtgaccggt ctttcttcaa gcatcacctc
     4381 agacagtgat tttgatggaa accccgacct catgtacagc gcctacgatt ttgcagggag
     4441 acgtctctta atggtttcaa atgggacatc aagaaggttc gacaatgtga agttggtggg
     4501 gccaggatac gagaaagatt ttgatcttga gccttatggg gtcaagataa tcatggagaa
     4561 tttggaggac aacagatgac caggtggaca ctcatagttg cttcattggt aatgatggca
     4621 ggcggtatat tgatgatggt catcaaggat ataaacggcc acccaattgg cgttccaagt
     4681 caaattgcac ccgtagttgg ctggatagtg ataatacttg gatttctgtt cctgatgttg
     4741 tcgatgataa gaagtgagta gcgacaaaag cgaagaaaaa aacctcgtag agagggcaaa
     4801 aaaaggcgaa gaatctgcct ttacagaaat tgttgaactt tacgctgaca aggttttctc
     4861 tgaagcctac tatatcatga acaatcgcga ggacgcgctt gacgcttccc agcttgtttt
     4921 tttaagggta ttccagaaca taaaacattt caaaggtgag tcactccttt caacatggat
     4981 ttacaggata acagtaaaca catgtttaag ggaattgaaa aaaagaaaca ggaaaccacc
     5041 tgaaccggat gacgatgacc cagacccgca agatgaagtc ataaggaatg aagaggaaaa
     5101 gatcgccatg gaaatactgg cgaaacttcc tgagccttac agagtcatat tgattatgag
     5161 ggagatgaat gacatgccct tcaaggacat ctcagatgtg ctaggcatct ccgtcaacct
     5221 ggccaaagta agggctttca gagccaaggc caggttcaaa caacttttgg aggaacaaga
     5281 gaatgggtaa tacggctttt ttgatgccag gccagggctc ccaatatgtc gggatgcttg
     5341 gcaagctgga cgcgtatcca aaggcccagc agatcatatc aaaagtgaac ggcattcttg
     5401 gtttcgacct c
//
LOCUS       DAOSRI010000108         5401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13828_length_5401_cov_3.621399, whole genome shotgun sequence.
ACCESSION   DAOSRI010000108 DAOSRI010000000
VERSION     DAOSRI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13828_length_5401_cov_3.621399"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /gene="rpsM"
                     /locus_tag="PK717_04185"
     CDS             complement(<1..167)
                     /gene="rpsM"
                     /locus_tag="PK717_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR46893.1"
                     /translation="MPRISGIDIPAGKRVDISLRYIYGIGPKNALEIVKKAGFDPNAK
                     MKDLTEAQIEK"
     gene            complement(187..300)
                     /gene="rpmJ"
                     /locus_tag="PK717_04190"
     CDS             complement(187..300)
                     /gene="rpmJ"
                     /locus_tag="PK717_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOR46894.1"
                     /translation="MKVRASVKPICEKCKIIRRKGRVMVICENPKHKQRQK"
     gene            complement(311..529)
                     /gene="infA"
                     /locus_tag="PK717_04195"
     CDS             complement(311..529)
                     /gene="infA"
                     /locus_tag="PK717_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005906834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR46895.1"
                     /translation="MADSKDVIETEAVVLETLPSAMFKVKLANGRVILAHISGRMRMN
                     FIRILPGDKVRIELTPYDLSRGRIIYRL"
     gene            complement(545..1291)
                     /gene="map"
                     /locus_tag="PK717_04200"
     CDS             complement(545..1291)
                     /gene="map"
                     /locus_tag="PK717_04200"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR46896.1"
                     /translation="MIVLKTPEEIAKMRLLGQQLARILHTLKQQAKAGMTTTELDEIA
                     SSETELIGARCASYGYGGFPGHCCISLNETVIHGIPSGRELKEGDLVSIDHVIERDGL
                     FVDSTITFTIGLPNRSQTRIMEVCSKALDEGIAIAKPGKRIGDIGNAVQLVVEGAGYS
                     IIRDFVGHGVGKSMHEDPQIPNFGRSGTGPIIVPGMTLAIEPMIAEGSYHVRIAKDGW
                     TANMADGKLSCHMEHTIAILENETVVLTKI"
     gene            complement(1288..1908)
                     /locus_tag="PK717_04205"
     CDS             complement(1288..1908)
                     /locus_tag="PK717_04205"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOR46897.1"
                     /translation="MNLVFLGPPGSGKGTASAFLSDKLGVPRFVASELLRAEIKTGSV
                     LGLTIATYMETGDLVPDDLVIKVVLKNLENVKGFILDGFPRTEKQAIALKEALAKLSM
                     KLDKVIYLDVSFETAKERNLARMICKSCGFSPQPTERACPRCGGVLIKRLDDNEETIK
                     RRFENYIKETQPLVDFYKDENILVRIDASSPAEIMNKQILTELGYR"
     gene            complement(1910..3220)
                     /gene="secY"
                     /locus_tag="PK717_04210"
     CDS             complement(1910..3220)
                     /gene="secY"
                     /locus_tag="PK717_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR46898.1"
                     /translation="MLKILRNAWSIPELRKKILVTLGLFLLFRLGTHISVPGVQRAVI
                     YMMSSQGGLVGLMDLFSGGGLLNISLFALGVMPYINSSIILQLLNVVIPSLEKLAKEG
                     GLEGQRKIAQITKYVTLGLAAIQSVSYAFFLYPSILNEMGIKVNLLVAPDSIMLKIFV
                     SFILFVGSFLLVWLGEVMTENGIGNGVSLIIFAGVISKILPGIGQVIENIRGGSATWF
                     GLIFGLAIFAVFIGAVIFAYEAERRVPVQYAKRIVGNRMYGGQSTYIPIRMIQGGVLP
                     IIFASSVMMFPQIIGRFFPADNWWNVIFVEPLKMQTSWLYNVIFFLLIVFFTYFYTSI
                     TYNPPELAENLRKQGGFIPGVRPGDATAKYFQSVLSKVTLPTAIFLGALAIIPNFILK
                     GNMQLNNLFVGGTGILIVIGVALETVAQIESFMTMRSYEGFLRK"
     gene            complement(3233..3727)
                     /gene="rplO"
                     /locus_tag="PK717_04215"
     CDS             complement(3233..3727)
                     /gene="rplO"
                     /locus_tag="PK717_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR46899.1"
                     /translation="MDKIKQPEGSKKEKWIIGRGMGSGSGRRCGYGHKGAKSRSGRGK
                     GYGSGFEGGQTKLFRRLPKIRGWRNQDVNPIRVAEVKLGQLNVIPAGTDVTIDVLRTY
                     DLAKKTDQAYKILGTGEITVSLNFNKAKATKSAVDAIKNAGGTIEIFEKKPLEKKAKK
                     TASK"
     gene            complement(3737..4273)
                     /gene="rpsE"
                     /locus_tag="PK717_04220"
     CDS             complement(3737..4273)
                     /gene="rpsE"
                     /locus_tag="PK717_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOR46900.1"
                     /translation="MAEHDRFFRDRDKEEENKFEEQIVQIARVSKTVKGGKRMRFRVL
                     VVVGNKEENSVGIGIGKAKEIPNAIRKAVNRAKREMVKVPLSGSTIPHDINIKNGSAR
                     IYLAPAVQGTGLKAGGAVRMVLEKAGVKDIVSKAHGTTNAVNLAKTTIEALRTLRDPK
                     KYAQERGITVAQLFGRQE"
     gene            complement(4282..4635)
                     /gene="rplR"
                     /locus_tag="PK717_04225"
     CDS             complement(4282..4635)
                     /gene="rplR"
                     /locus_tag="PK717_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033008681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR46901.1"
                     /translation="MYEPINKNAERLRRKATIRKKVHGTSNRPRLAVFKSLKHIAVQI
                     INDDEGKTLVMVSSKNEGLKGKNIQVAEKVGTIIGQRALEAGIKQVVFDRSGYIYHGK
                     VKALADSARKTGLEF"
     gene            complement(4639..5196)
                     /gene="rplF"
                     /locus_tag="PK717_04230"
     CDS             complement(4639..5196)
                     /gene="rplF"
                     /locus_tag="PK717_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR46902.1"
                     /translation="MSRIGNKPIELPKGVTVDIQDTVLGQKVTVKGPKGTLERTFRPE
                     IKIMLEGNILTTTRSSNLKFVHQLHGTTRAHLNNMVIGVTEGFKKVLLISGVGFKAKA
                     EGNKVTFNLGFSHDIKLDVPGGLKVATPKENRIEVEGFDKELLGAFAQKIRMLRDPDP
                     YKAKGVTYEGEVIRRKAGKAMTKGA"
     gene            complement(5207..>5401)
                     /gene="rpsH"
                     /locus_tag="PK717_04235"
     CDS             complement(5207..>5401)
                     /gene="rpsH"
                     /locus_tag="PK717_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR46903.1"
                     /translation="KRDRIITGITRISHCGRRVYVKHDAIPKILRGMGVIIMSTPKGV
                     MTGHQAEKTHQGGEVLAAIW"
BASE COUNT         1279 a         1357 c         1158 g         1607 t
ORIGIN      
        1 atcttctcga tctgcgcctc ggtaaggtcc ttcatcttgg catttggatc aaatcctgcc
       61 ttcttgacta tctcaagcgc attctttgga ccaatgccat agatgtagcg aagcgaaatg
      121 tcaactcttt ttcctgccgg tatgtcaata cctgaaatac gtggcatact tgcctcctaa
      181 gtattcttac ttctggcgct gcttatgctt cgggttttcg caaattacca tcacgcggcc
      241 cttgcgcctg attatcttac acttttcaca aatcggtttt acagaagccc ttactttcat
      301 ttcatcctcc ttagagccta tagataatgc ggcctcgtga gagatcgtaa ggagtgagct
      361 ctattctcac tttatcccct ggcaggatcc tgatgaaatt catcctcatc ctaccggaga
      421 tgtgggccag aataactcta ccattggcga gcttcacctt aaacatcgcc gatggcagtg
      481 tttccagaac caccgcttcg gtctctatca cgtcttttga gtcagccaca aaacctccta
      541 acagttaaat tttggtcaaa acaacagttt cattttccaa tatcgctatc gtgtgttcca
      601 tgtggcacga aagcttgcca tcagccatgt tggctgtcca gccatctttt gctattctca
      661 catgatacga tccttcagcg atcattggtt caattgccaa ggtcatgcct ggtacaatta
      721 ttggccctgt tcctgatctg ccaaaattcg gtatttgagg atcttcatgc atggatttgc
      781 ctactccatg ccctacaaaa tcccttatta tcgaatatcc ggcaccttca acgaccaatt
      841 ggacagcgtt tccgatgtcc ccgattcttt ttccaggttt cgctatggct attccttcat
      901 caagagcttt ggaacaaact tccatgattc ttgtttgaga acgatttggc aaaccaattg
      961 taaaagtaat ggttgaatca acaaataaac catccctctc tataacatgg tctattgata
     1021 ccaaatcacc ttcttttagt tcccttccag aaggaatgcc gtgtatgact gtttcattga
     1081 gcgaaatgca acaatggcca gggaaacctc cgtagccata acttgcgcac ctggcaccaa
     1141 taagttcggt ctcgctggaa gcaatctcat ccagctcggt cgtggtcata cctgccttgg
     1201 cttgctgttt caaggtgtga aggatgcggg ccaactgctg gccaagcaac ctcatttttg
     1261 caatctcttc cggagttttt agaacaatca tctataccca agttcagtga gtatctgttt
     1321 gttcattatc tctgccggag agctcgcatc aatccgaaca agtatattct catccttgta
     1381 gaagtcaaca aggggttgag tttcttttat gtagttttca aaccttcttt taatggtttc
     1441 ctcattgtca tccagccttt ttatcagaac accaccacat cttgggcagg ccctttcagt
     1501 tggctgggga gaaaatccac aagacttgca tatcatgcga gcaaggttcc gctccttggc
     1561 tgtttcaaat gaaacgtcaa gatagatgac cttgtccagc ttcattgaga gctttgcaag
     1621 cgcttctttt agagcaattg cctgtttctc agtcctgggg aaaccatcca gtataaatcc
     1681 ttttacgttt tcaaggtttt ttaacacgac ctttatcaca aggtcgtcgg gtaccaggtc
     1741 acctgtttcc atgtaggtgg cgattgtcag gccaagtact gatccagtct ttatttctgc
     1801 cctgaggagt tcggaggcaa caaacctagg tactccaagc ttatcggaca gaaaggcgct
     1861 tgcggttcct tttcccgaac ctggcggacc caaaaacact aaattcatct tatttcctca
     1921 ggaacccctc gtaagaacgc atcgtcatga aggattctat ttgtgcaact gtttcgagtg
     1981 ctacaccgat tacgatgagg attccggttc caccaacaaa caggttattg agctgcatgt
     2041 ttcccttgag aataaagttt ggaatgattg ccaaggcacc aaggaaaata gctgttggga
     2101 gtgtgacctt tgaaagaact gattggaagt actttgcagt ggcatcacca ggcctgacgc
     2161 cagggataaa tccaccctgt tttctaaggt tctctgcaag ctccggagga ttgtaggtaa
     2221 tagaggtata gaaataggtg aagaagacta tcaacaggaa gaagataaca ttatagagcc
     2281 atgaagtttg catcttgagt ggttctacaa aaatcacatt ccaccagttg tctgccggga
     2341 aaaaccttcc aattatctgc gggaacatca ttaccgaaga ggcaaagatg attggaagaa
     2401 ctccaccctg aatcatcctt atcgggatgt aagtgctctg tccaccatac atcctgtttc
     2461 caactattct cttggcatac tgcaccggta ctcgtctttc cgcttcatat gcgaagatga
     2521 ctgctccgat gaaaactgca aagatggcca aaccaaagat gagcccaaac caggtggcac
     2581 ttccacccct gatgttttca attacctgac caatgcccgg taggattttc gaaataacgc
     2641 cggcaaagat gatgagcgaa acgccgtttc caatcccgtt ctcagtcatt acttcaccga
     2701 gccagaccag caagaacgag cccacaaaca atataaagga aacaaatatc ttgagcatga
     2761 ttgaatcagg ggccacaaga agattgacct ttatacccat ctcgttaagg atgcttggat
     2821 aaaggaagaa agcataagaa actgactgga ttgcagcaag accaagagtc acatacttcg
     2881 ttatctgggc aatcttgcgc tggccctcga gaccaccctc ttttgccagt ttctcaagtg
     2941 atggaataac cacattgagt agctggagga taatcgagga gttgatatat ggcattacgc
     3001 ccaaggcaaa gagtgaaatg ttgagcaaac caccacccga gaacagatcc atgagaccaa
     3061 cgagtccgcc ctgggagctc atcatgtaga tgactgctct ctggacacct gggaccgaga
     3121 tgtgtgttcc aagcctgaaa aggaggaaga gtcccaatgt caccagaatt tttttacgga
     3181 gttcaggtat gctccaggcg tttctcaaga tttttagcat tgtgcgcgct ccttactttg
     3241 aggcagtctt ctttgctttt ttctcgagcg gcttcttttc aaaaatctcg atcgtgccgc
     3301 ctgcattttt aatggcatca acagccgact ttgtggcctt tgccttattg aagttaaggg
     3361 agacggttat ctctccggta ccaaggattt tataggcctg gtcagtcttc tttgccagat
     3421 cataagtcct gaggacatca atggtcacgt cagttcccgc cggaataacg ttaagctgtc
     3481 caagtttgac ctcggcgact cttatcgggt ttacatcctg gttgcgccat cccctgatct
     3541 tcgggagcct gcggaaaagc tttgtctgtc caccctcgaa gccggaccca tagcccttgc
     3601 ctctgcctga gcggctcttg gcacctttat gaccgtagcc acagcgcctt ccggaaccgg
     3661 agcccatgcc ccttccgatt atccactttt ccttttttga gccttcgggt tgtttaattt
     3721 tgtccaaaga cattgtttat tcctgccttc caaatagctg ggcaacggtt attccgcgct
     3781 cctgagcata tttcttcggg tccctgagtg ttctgagggc ttcaatggtt gtcttggcca
     3841 ggttgactgc atttgttgtt ccatgggctt ttgaaacgat gtctttgaca cctgcttttt
     3901 caagtaccat acgaacagca ccaccggctt ttagaccagt tccctgtact gccggagcaa
     3961 gataaattct agccgagcca ttttttatgt tgatgtcgtg gggaattgtc gatccagaaa
     4021 gcggtacctt gaccatctcg cgtttggctc tgttgacggc cttgcggatg gcgttcggga
     4081 tttcttttgc tttcccgata ccaattccca cagaattctc ttctttattg ccaacgacaa
     4141 cgaggaccct gaatctcatc ctcttaccgc cttttacggt cttggagacc ctggcaatct
     4201 ggacaatttg ttcttcaaac ttgttttctt cttctttgtc cctatctctg aaaaatctgt
     4261 cgtgctctgc catgtctcct cctagaactc aagcccggtt tttctggccg aatcggcaag
     4321 ggctttgact ttgccatgat atatataacc tgatcgatcg aaaacaacct gtttaatgcc
     4381 ggcttcaagt gcacgctggc caatgattgt accgaccttc tcggctacct ggatgttttt
     4441 gcccttgagt ccttcatttt tgcttgagac cataacaagg gttttaccct cgtcatcatt
     4501 aatgatctgg actgcaatgt gcttgaggct cttgaatacc gcaagtctcg ggcgatttga
     4561 agtgccatga acttttttac ggatggttgc ttttcttctc aatctttcgg catttttatt
     4621 tatcggttcg tacatgtgtt aggctccctt agtcattgcc ttgccggctt ttcttctaat
     4681 gacttcacct tcataggtga caccctttgc tttatagggg tccggatcac ggagcatgcg
     4741 gatcttctgg gcaaatgcac caagaagttc cttgtcaaac ccttctacct cgatcctgtt
     4801 ttcctttggt gtggcaacct tgagtcctcc aggaacatca agctttatgt cgtgggaaaa
     4861 accaaggttg aaagtgacct tattgccctc agcttttgcc ttgaagccaa cacctgagat
     4921 gagaaggacc tttttaaacc cttcggttac accaatgacc atattgttga gatgggccct
     4981 agttgttcca tgaagctgat ggacaaattt caggtttgag gagcgggttg ttgtaaggat
     5041 gtttccttca agcatgatct tgatctctgg cctgaatgtt ctctcaagag tgccttttgg
     5101 ccccttgact gtgaccttct gccccaagac tgtgtcctga atatcgactg ttacgccctt
     5161 tggcagttca attggtttgt ttcctatcct tgacatggtt gcactcctac cagatggcgg
     5221 caagcacttc gccaccctga tgggtttttt cggcctggtg gccggtcatt acgcccttcg
     5281 gggtactcat gatgatcaca cccatgcccc tgaggatctt tggtatggcg tcatgcttga
     5341 cgtataccct gcggccacag tggcttatcc tggtgatacc agtaatgatg cgatctctct
     5401 t
//
LOCUS       DAOSRI010000109         5395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13847_length_5395_cov_4.031086, whole genome shotgun sequence.
ACCESSION   DAOSRI010000109 DAOSRI010000000
VERSION     DAOSRI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13847_length_5395_cov_4.031086"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="selA"
                     /locus_tag="PK717_04240"
     CDS             <1..829
                     /gene="selA"
                     /locus_tag="PK717_04240"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOR46904.1"
                     /translation="PEVMTASGAMLHEVGTTNRTHLKDYAGAINEKTGLILKVHPSNY
                     AIRGFASSPTTAELSALAKEKNVPFVYDVGSGLLRKIDHPNFREEPDCLSALSDGADA
                     ILFSGDKILGGTQAGFIVGSEKIAGACGKIPLMRSLRLAKAHMCMLEEALRTWLLPDR
                     EVMKLNPVMRMISMMPEELEARANTLSGEINKANPAIGSFVKKVEGYMGGGSLPDINF
                     DSYAVAIKPKETPQLLLQKLRNSETPIVARIEDDLVLFDMRTVLDGEGEKIARALAQ"
     gene            826..1470
                     /gene="nth"
                     /locus_tag="PK717_04245"
     CDS             826..1470
                     /gene="nth"
                     /locus_tag="PK717_04245"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR46905.1"
                     /translation="MTQSEIATRAIEKLARFWGGFEFADFNKRDPWRILVSAIISHRT
                     QDNVTYPATERLFSKWFDVRSLSLADPDEVAKVIYPAGFYKTKSQRLVQMAIFITSQY
                     SGKVPDSLDELLCIKGVGRKTANLVLSLGYNIPSVVVDIHVHRISNRAGWVKTQTPEQ
                     TEFELMKLLPKKYWTKSNELLVRLGQNICKPIVPKCEQCPICPMCVMGSSVKKN"
     gene            1548..1943
                     /gene="speD"
                     /locus_tag="PK717_04250"
     CDS             1548..1943
                     /gene="speD"
                     /locus_tag="PK717_04250"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOR46906.1"
                     /translation="MYTYGRHLLCEVSGCDPSIIGDVDKVKEILYQAAGLAKAEIMES
                     TFHHFSPQGVSGVVVIAESHLSIHTWPEFGYAALDIYTCGEHTDPEGALNYCSEKFGA
                     SEVDITRIERGLKEETGGFSHRLSNVGAA"
     gene            2127..2396
                     /locus_tag="PK717_04255"
     CDS             2127..2396
                     /locus_tag="PK717_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOR46907.1"
                     /translation="MKDNEARSSVIKRLSKIEGQVRGLVRMISEERSCDEIINQIQAV
                     KAGINKVAAIILERNIVDCLSLSENTDQTEIEKLRKLLSKIAGSK"
     gene            2421..3893
                     /locus_tag="PK717_04260"
     CDS             2421..3893
                     /locus_tag="PK717_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR46908.1"
                     /translation="MKRELLDELMGLGIDIVSTPFECQFYGFDVTPLPKETSWVFRGT
                     TPDAVLRPKNTEEVVKIMDFANKHKVPVCPRGGGTSGYYQSVPRGKGIVIETLALNSI
                     GELDEKEGLISVGGGVIWSQLDWELKKKGWTLKTYPTSYKSSTVAGWTQTEGLGVGSL
                     AFGSIKKLIKEIEVVLPSGEVVWIPNEGLVETKSGNLKFEDFIKSEGMMGIISSVKLE
                     VRRLPESTVTYLLKFKDKNDFALVSSRLAEISSIFFIEFVNGSYMDLLAQSGHHTPKH
                     LKEEVFAVIRLEGGKSDVEKGEKEIAALLAEHTEIAQLPQEEAEEEYGYRLKYFRIKN
                     AYSSVTPADLSVPLSNLGQYLDKVSRFRFEFAYKGEILTKEQCGLMFFYVLANELSFV
                     RFMSAAPYQMEFILSAMKMGGSPNGGIGLLNTPYIFGLRTQSEREAFIQKKEMFDQNW
                     IMNPGKWTDPPFFLRPSIYFSGMKLLEPVCWLVGGIVGRW"
     gene            3895..5097
                     /locus_tag="PK717_04265"
     CDS             3895..5097
                     /locus_tag="PK717_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR46909.1"
                     /translation="MKKYAAIEKQVRKCIQCGSCRVECPTGDDVGWISCSPRAKVLFI
                     RDFLLGRKPTKVKMSEEFAQRLFQCTLCGRCGVECITEIDFPEVWFEIRELVQDEGVK
                     LPIVEMVSGIIKDTKNVANQPNDDRPNWLKRAKLASDPTKVEKADVVYYVGCMSSFYP
                     QSQSMARAFAQILEASGTNFAIMGTSEHCCGFPMISTGKVHEAKELMYHNQEEIKKYG
                     AKTVVATCPACTSMWKKEYPKLSGKPNDFEVMHATQFVLKLMKENKLKLIPPKQEIQV
                     TFHDGCDLGRNGGVYDEPREILKMLPGVMFTEHSESKDISVCCGAGGDLLMTNPEMAD
                     RIGQKRARSLSEVAPVAVTCCPSCKKQLKDAAKHAEVKLDTKDLLEMVADMLEKPEKP
                     EKPEKTEA"
BASE COUNT         1597 a         1086 c         1354 g         1358 t
ORIGIN      
        1 gccggaagtg atgactgctt cgggggcaat gcttcatgag gttggaacca caaacaggac
       61 gcacctgaag gattatgcag gagcaatcaa tgaaaaaacc gggctgatcc tgaaagtcca
      121 cccttcgaac tatgcaataa gggggtttgc atcttctccg acaactgccg agttgtctgc
      181 tctggcaaaa gagaagaatg ttccatttgt ttatgatgtt ggctcgggcc ttttgcgcaa
      241 gatagaccat cccaatttca gggaggagcc ggactgcctc tcggcattga gtgatggcgc
      301 agatgcaata cttttttccg gtgacaagat acttggcgga acacaggcgg gttttattgt
      361 cggaagtgaa aaaattgctg gtgcctgtgg taaaatcccc ctcatgagga gcctgaggct
      421 tgcgaaggcc cacatgtgca tgctcgaaga agcattgagg acatggttgc taccagacag
      481 ggaagtcatg aagctgaacc ctgtcatgag aatgatatca atgatgcctg aggagctgga
      541 agcaagggca aacactctct ctggggaaat caacaaggca aatccggcaa tcgggtcttt
      601 tgtaaagaag gttgaaggct acatgggcgg tggctcactg cctgacatca attttgattc
      661 ttatgcggtg gccataaaac caaaagaaac ccctcagttg ttgctgcaaa agttgagaaa
      721 ttctgaaact ccgattgtgg caaggattga agatgacctt gtcctttttg acatgagaac
      781 agtacttgat ggtgaaggag aaaaaatagc cagggcactc gcgcaatgac ccagtccgaa
      841 atagcaacca gggccattga aaaattggcc agattttggg gagggtttga attcgcagat
      901 ttcaacaagc gtgacccgtg gagaatactg gtttccgcaa taatttccca cagaacacag
      961 gacaacgtaa catatcctgc aaccgagagg cttttttcaa aatggttcga tgtcagatcg
     1021 ctttcgcttg ctgatcctga tgaggttgcc aaggtaatat atccagcggg gttctacaaa
     1081 accaagtccc aaaggcttgt ccagatggcc atttttatca cttcccaata tagtgggaaa
     1141 gtacccgatt ccttggacga gctattatgc ataaaaggtg tgggcagaaa gacagcaaat
     1201 ctcgtgcttt ctctcggtta caatatcccg tcggttgttg ttgatattca tgtgcacagg
     1261 atttccaaca gggcggggtg ggttaaaaca cagactccag agcaaacaga gtttgaattg
     1321 atgaagcttt tgcccaaaaa atactggaca aagtcaaatg agctcttggt tcgtttgggt
     1381 caaaacattt gcaaacccat cgttccaaaa tgtgaacaat gcccgatctg tccaatgtgc
     1441 gttatgggta gttctgttaa gaaaaattga tgttgatttt taacagactc aatatacaat
     1501 gcgactcgct atttttttac tatctctcgt gaagggggta caatgagttg tacacatatg
     1561 gaagacacct tctgtgcgag gtctctggtt gtgacccgtc tataataggt gatgtagaca
     1621 aggtgaagga gatcctttac caggctgccg gtcttgccaa ggctgagatc atggaatcaa
     1681 cattccacca cttttcacca cagggagttt caggtgtggt tgttattgca gaatcgcatc
     1741 tctcaattca cacctggcct gaatttggct acgcagctct tgatatctac acatgcgggg
     1801 aacataccga tccagagggc gccctcaatt attgctccga gaagtttggc gccagtgaag
     1861 tcgatattac aagaatcgaa cgcggtctaa aggaggaaac cggtggcttt tcgcaccggt
     1921 tgagtaacgt cggcgccgct tgataggcgg ctgcaaatac tctgggaaga tcggatttaa
     1981 tatttctcta gtcgtaaaac aatccattaa aattgaataa accagtttgt ttttaaataa
     2041 aaacaaatga tagtggttta ttttttaatg tcatgaaatg aagactgaca ttgacttatt
     2101 tgtgaatttt ggctagagtt gagcaagtga aagacaatga agccagatca tcagttataa
     2161 agaggctctc aaagatagaa ggtcaggtga gaggccttgt gcgcatgata tctgaagaac
     2221 gatcatgtga tgaaataatc aatcagatcc aggccgtaaa ggccggaata aacaaagtcg
     2281 cagcaatcat cctggaaaga aacatcgttg attgtttaag tctttcggaa aatacagacc
     2341 aaacagaaat tgagaagctc agaaagcttc tctccaaaat tgcaggtagt aaatgatata
     2401 gttgccgaaa ggagcgcttt gtgaagagag agcttttgga tgaattgatg ggtcttggga
     2461 ttgatattgt atcaacaccc tttgaatgcc agttttatgg ttttgatgtg accccgttgc
     2521 cgaaggaaac ctcctgggtt tttaggggga caactccaga cgcagtcctc agaccaaaaa
     2581 acaccgaaga agttgtcaaa ataatggatt ttgccaacaa gcacaaagtc cctgtatgtc
     2641 caaggggtgg cggaacctct ggatattacc agtcggttcc gagaggcaag gggattgtta
     2701 ttgaaacatt ggctttaaat agcataggtg agcttgacga aaaagaggga cttatcagtg
     2761 ttggtggtgg agtaatatgg tctcagcttg actgggaatt gaagaaaaag ggctggacgc
     2821 tcaagacata ccccacttcc tataaatcat ccactgttgc tggctggacc cagacagaag
     2881 gccttggtgt gggaagcctc gcttttggct ccataaagaa actgataaaa gagatcgagg
     2941 ttgttcttcc aagcggtgag gttgtatgga tcccaaatga aggtctcgta gaaaccaaat
     3001 caggcaacct gaaatttgaa gattttataa agagtgaagg catgatgggc atcattagct
     3061 ctgtaaaact ggaggtaagg cgacttcctg agtccactgt aacgtacctc ctgaaattca
     3121 aagacaaaaa tgattttgca ctggtctctt cgcgactggc cgagatttct tcaatatttt
     3181 tcatcgagtt tgtaaatggc tcttatatgg acttgcttgc gcagtcaggc catcatacgc
     3241 caaaacattt gaaggaagag gtgtttgcgg ttattaggct tgaaggcggt aaatcggatg
     3301 ttgaaaaggg cgagaaagag attgccgcgc tgcttgccga acatacagaa attgctcagt
     3361 tgccacagga agaagctgag gaagagtacg gctacagatt aaaatatttc agaataaaga
     3421 acgcctactc atcggtaaca ccagcagatc tgtctgttcc gctctcaaat cttgggcaat
     3481 acctggacaa ggtttcgcgc ttcaggttcg aatttgcata taagggcgaa attctaacaa
     3541 aggagcagtg cggactgatg tttttctatg tcctagcaaa cgagctctct tttgtcagat
     3601 tcatgtcggc tgcaccgtac cagatggaat ttatcctctc tgcgatgaaa atgggtggca
     3661 gcccaaacgg tgggattgga ttgctcaata ctccttatat ttttgggttg agaacgcaat
     3721 cagaaagaga agcatttatc caaaaaaaag agatgtttga ccaaaactgg ataatgaacc
     3781 ctggcaaatg gacagatcca ccgtttttcc tccggccatc gatttatttt agtggaatga
     3841 aactcctgga gccggtttgc tggcttgttg gcgggattgt ggggaggtgg tagcatgaaa
     3901 aaatatgcag ccattgaaaa acaggtcaga aaatgcatac agtgtggctc atgccgtgtg
     3961 gaatgtccca ctggtgacga tgttggctgg atttcatgtt ctccaagggc aaaagtcctt
     4021 tttataagag actttctact tggccgcaaa cccacaaaag tcaagatgtc ggaggaattt
     4081 gcccagaggc ttttccagtg cacgctttgt ggaagatgtg gcgtcgagtg cataacggaa
     4141 atcgatttcc ccgaggtctg gtttgaaata agggagctgg tccaggatga aggtgttaaa
     4201 ctcccgattg tggaaatggt ctccggaatt atcaaagaca caaagaatgt tgccaaccag
     4261 ccaaacgatg acaggccgaa ctggctcaag agggcaaaac ttgcctccga tccgacaaaa
     4321 gtcgaaaagg ccgatgttgt gtactatgtt gggtgcatga gttcctttta tccgcagtca
     4381 cagagcatgg ccagggcctt tgcccagata ttggaagcat caggcacaaa cttcgcaatc
     4441 atgggaacga gcgagcattg ctgcgggttt ccgatgattt caaccggaaa agtacatgaa
     4501 gccaaagagt tgatgtacca taaccaggaa gagataaaga aatatggcgc aaaaactgtt
     4561 gtggccactt gtccagcctg caccagcatg tggaagaaag aatatccaaa gctctccggc
     4621 aaaccgaatg attttgaagt gatgcatgca acccagtttg tcctgaagct gatgaaggaa
     4681 aataagctca agctcattcc gccaaagcag gaaatacagg tgaccttcca tgatgggtgt
     4741 gatcttggaa ggaacggcgg cgtttatgac gagccaaggg aaatacttaa aatgctccct
     4801 ggcgtaatgt tcaccgagca ttctgaaagc aaggacattt cggtttgctg tggcgccggt
     4861 ggcgacctgc tcatgacaaa tcctgaaatg gccgacagga ttggacaaaa gagggcaagg
     4921 agtctttctg aggttgcgcc agttgcggtg acctgttgcc cgtcctgcaa aaaacagctc
     4981 aaagatgcag caaaacatgc cgaggtcaaa cttgacacca aagacctcct ggaaatggtt
     5041 gcagacatgc ttgagaaacc cgaaaaacca gaaaagccag agaaaacaga agcttgatat
     5101 ttacacctaa aaagtcaaag gcccccatga acatgggggc cttttttatt tctggttttt
     5161 ttatgccttg ttgcacaaaa cagcaaaaca aatcaaaatc aaaaaataca tttcagtaga
     5221 atgtatttca gttgacaaat gatagactac atcgacaaaa tacagggtgc aataaacttt
     5281 atggaagcaa gactcaagga gagagtgggg cttggtgata ttgcaaaaga agctgggttt
     5341 tcctcttttc atttccacag gatattcctt gccaatgtcg gtgaaacggt cactg
//
LOCUS       DAOSRI010000110         5388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_13871_length_5388_cov_3.909619, whole genome shotgun sequence.
ACCESSION   DAOSRI010000110 DAOSRI010000000
VERSION     DAOSRI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_5388_cov_3.909619"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PK717_04270"
     CDS             complement(<1..1387)
                     /locus_tag="PK717_04270"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOR46910.1"
                     /translation="MMDAAYNPGQVEEKIYKFWMDGGFFESHPDKTKKPFVIMMPLPN
                     VTGVLHTGHALNNGLQDLCTRYHRMKGENALYLPGVDHAGIATQIVVEKELYKTEGKS
                     RFDIGREEFVKRVWQWKERCGNTIVNQQKRLGISCDWSHFRFTMDEPYQKAVREVFCH
                     YYEKGLIYRGKKVINWCPRCQTVISDIEVEHEDAQGNLWHIKYPFADNPEDGIIVATT
                     RPETMLGDTAVAVNPSDERYRDLVGKKVILPIAGRDIPIIADEYVDREFGSGAVKITP
                     AHDPNDFEVGRRHDLPMPVVMGPDGRMIDVPERFVGLTMLEARKKIVAELEEKGYLIK
                     IEPHSHAVGHHDKCKTVIEPLLSPQLFLKMKELAQPAIDIVREKGVRFTPERYTKVYF
                     DWMENILDWPISRQCWWGHRIPIFTCEKCNHEFASRVDVERCPKCGGKVHQDEDALDT
                     WFSSALWPFATL"
     gene            complement(1527..2684)
                     /locus_tag="PK717_04275"
     CDS             complement(1527..2684)
                     /locus_tag="PK717_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46911.1"
                     /translation="MSGIYQGFKIGHKYKEENMKSKYIVPIILAFTLLFGNVASYSRQ
                     NDNETMFSTNGRSFEIVAYKYMGGNKPYEYGKPSPLLVCEDGELTFYEFECKDIEGCR
                     QVVPKLDKDGYPIKIQKNDKDILEKLFVTLNPRIVAKIENTKLRINAIALGIFVGTQW
                     NFNIYTKDLVEEFKLRMDASGTDKIYPYGSISIMDDNYNFEVYEYYGVMHQRTRVESL
                     NPDCCEYLSDYPGLYKLGEALYSTGGFGQGYLWGKVDSNQFKIAGRDYEWIMPAFVWG
                     EGGLFVNLLFLTDVLFAPYTSDGKTYTIKRLFRKQSRNETILIELQLTKNSKKAYLNG
                     QEVELSLEPFDTKDAFYVPLLDVCRLLRANCHTSRYDGSILIARFFAPMND"
     gene            complement(2647..2856)
                     /locus_tag="PK717_04280"
     CDS             complement(2647..2856)
                     /locus_tag="PK717_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46912.1"
                     /translation="MLASSTTTAGLFSGDITFSTSGTGKTYDRSDTGYASMYRDNLHL
                     IAFDNNNDGIVDYYEWDLSGLQNWP"
     gene            complement(2869..3999)
                     /locus_tag="PK717_04285"
     CDS             complement(2869..3999)
                     /locus_tag="PK717_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46913.1"
                     /translation="MKKTIIFLLASIILVSPLGHAKSLNKEAAPSQFPDFKIVVDKDY
                     SFEDLDKKYTIMEHTKEEGLVFYRLEKKQTAKSFAYLPVLDKNNNPVKLSPKDPDYCY
                     EVAKALNVSIAAVQLETESDILAVSIVFYNYGDYEFYTALTQSSFSKHGTGSSGFVSF
                     ENMHKYKMLSKGSAYIIDTNLNIEYLRLEDKEYKITHETLNPDLEENNIIYAIDNVVT
                     HTYSSYDNVGGYIHLQLDNPIYTIAGKPIKYYPPPTLKDGQLMVGLEIVNKMGAFYER
                     IKDTNGYRIVKADKAIHGPITVTDITITIGEKKAIVDGQEYELSAIPYYYTVKPYNDN
                     PMIPLRDFCYLLNNELHWRPIDNSALIDRFQPIDPREPLPKK"
     gene            complement(4064..5170)
                     /locus_tag="PK717_04290"
     CDS             complement(4064..5170)
                     /locus_tag="PK717_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46914.1"
                     /translation="MNVILNLRKFVLVLVVFAIIVTPYSSPSFGIQKMIWPQDPFTII
                     AYEYRSFSGGSFWSFKKPTPMLSYEKGKLTFYEFEIVEDETGKHYSPVLDEYGSPVKV
                     DPADKDIVKKAEKIITSRRLFGKLDGPQGRIALGMFFQEDDNYYKWKYCIFGKDINRS
                     QYISGTDKIYPVGSISYLDGDYNFEVQAYHGYAYQVERREALNPEYYDRYEIPGLVDF
                     GEASYINRQSGAGYLWLSAKDPKVVINTVEHSYTNFALVLSENQIPFVALSFLSDVLF
                     APMTGDGETYTIQRLYNIKTTEVELVKLTLTKDSKKALIDGKEVELSEPPFMTDTGLF
                     VPLLDVCKLLKANCHYRKTDYSAIVARFFAPMNE"
BASE COUNT         1400 a         1186 c         1124 g         1678 t
ORIGIN      
        1 caagcgtggc aaaaggccag agggcagacg agaaccatgt atcaagggca tcttcgtcct
       61 ggtgcacctt tccaccacac tttgggcacc tttccacgtc gacccttgag gcaaattcat
      121 ggttgcattt ttcgcaggtg aaaattggaa tcctgtggcc ccaccagcac tgccttgaga
      181 tgggccagtc gaggatgttt tccatccagt caaaatagac cttcgtgtat ctctccggtg
      241 tgaacctgac acccttttcc ctgacgatgt cgatggctgg ctgtgcaagt tccttcattt
      301 tcaagaaaag ctgcggagag aggagtggtt caattactgt cttgcacttg tcgtggtggc
      361 caacagcgtg tgaatggggt tcaattttta tgagataacc tttttcctcg agttcggcaa
      421 cgattttctt ccttgcctca agcattgtga ggccaacaaa tctttccggg acatcgatca
      481 tcctgccatc aggccccatt acgactggca tcgggaggtc gtgcctcctg ccaacctcaa
      541 agtcattcgg gtcgtgggct ggtgtgatct tcacggcgcc actgccaaac tccctgtcta
      601 catattcatc ggcaatgatc gggatgtccc ttccagcaat cgggaggatg acttttttcc
      661 cgacgaggtc cctgtatctc tcgtcgctgg gatttacagc aacagcagtg tctccaagca
      721 tggtttccgg ccttgttgtg gcgacaatga ttccgtcttc aggattgtcg gcaaacgggt
      781 attttatgtg ccagaggttg ccctgtgcgt cctcgtgctc cacctcgatg tctgatatga
      841 cagtctggca cctcgggcac cagttgatta cttttttgcc cctgtaaatg aggccttttt
      901 cataatagtg gcagaaaacc tcgcgcactg ctttctggta gggctcgtcc atggtgaagc
      961 ggaagtgcga ccagtcgcat gaaatgccaa ggcgcttttg ctggttgaca atggtgttgc
     1021 cgcacctttc cttccactgc cagacccttt tcacaaactc ttccctgccg atgtcaaacc
     1081 ttgactttcc ctcggttttg tagagttcct tctcgacgac gatctgggtc gcaatgccgg
     1141 cgtggtcaac accgggcaga taaagggcgt tttcaccctt cattctgtgg tatcttgtgc
     1201 agaggtcctg caggccgttg ttcagggcgt ggccggtgtg caggacgccg gtaacgtttg
     1261 gaagaggcat catgatgaca aagggttttt ttgtcttgtc cgggtgcgac tcaaaaaatc
     1321 cgccatccat ccagaattta tatatctttt cttcaacctg ccccgggttg taggctgcgt
     1381 ccatcatggg gacctcctgt tagtgtgcgt catgaccaat ggattttgcc agcaaggacc
     1441 agccaagtca agaatttgtt gctaacaaag agcaaaaaat tgtctatatt tttttatgga
     1501 ttaatttttt gtggagctga gggactctag tcgttcattg gggcgaagaa tctggcgatg
     1561 aggattgacc catcatacct actggtgtgg cagtttgccc tgagaagcct gcagacatcc
     1621 aggagtggaa cataaaaagc gtctttcgta tcaaacggct caagcgaaag ttccacttcc
     1681 tggccattta gataagcttt tttgctgttt tttgttagtt gtagctcgat aaggatggtt
     1741 tcattgcgag attgtttacg aaacaatcgt tttatggtgt atgtcttccc atcagaagta
     1801 tatggtgcaa acaaaacatc tgtaaggaat aaaagattta caaacaaacc tccttcaccc
     1861 caaacaaatg ctggcataat ccattcataa tctcttccgg caattttaaa ctgattagaa
     1921 tcaaccttac cccataggta tccttgacca aaaccacctg tactatataa tgcctcacct
     1981 agcttgtata atccaggata atcagaaagg tattcgcaac aatcagggtt aagtgactca
     2041 acccttgttc tttgatgcat aacaccatag tattcataaa cctcaaaatt gtaattatca
     2101 tccattatcg atattgaacc ataaggatag atcttatctg tacctgaagc atccatcctt
     2161 aacttaaatt cttccaccaa atccttggtg tatatgttga aattccattg tgttccaaca
     2221 aatataccaa gggcaatggc attgattctc agttttgtat tttcaatttt ggcaactatt
     2281 cttggattta gtgtaacaaa caatttctca agtatatctt tatcattttt ttgaattttt
     2341 atcggataac cgtctttgtc cagttttggg acaacctggc ggcacccttc aatatctttg
     2401 cattcaaatt cgtaaaatgt cagctccccg tcttcacaaa ccagcaatgg ggacggtttg
     2461 ccatactcat agggtttatt tccacccata tatttgtatg caactatctc aaatgacctg
     2521 ccattagttg aaaacattgt ttcattatca ttttgtctgc tgtagctggc tacattgcca
     2581 aatagtaatg tgaatgcaag aattattggt acaatatatt tacttttcat attttcctcc
     2641 ttatatttat ggccaatttt gaagccctga tagatcccac tcatagtaat ctacaatacc
     2701 atcattgtta ttatcaaaag ctatcaagtg aagattatct cgatacattg atgcatatcc
     2761 agtatccgac ctatcgtaag ttttaccagt tccagaagta gaaaaagtta tgtcaccaga
     2821 gaagaggcca gctgtggtgg tggagctggc aagcatgatt ttatatcatc atttctttgg
     2881 caatggctcc ctcggatcta tcggttggaa tctatcaata agggcagaat tgtcaattgg
     2941 tctccagtga agctcattgt taaggagata gcaaaaatca cgcaggggta tcattggatt
     3001 atcattgtaa ggttttacag tataatagta cggtatggca ctgagttcat actcctggcc
     3061 atcaacaatg gcctttttct caccgattgt aatggtgatg tcggttaccg ttattggccc
     3121 atggattgct ttatcagcct tgacaatcct gtatccattt gtatctttta ttctctcgta
     3181 aaatgcaccc attttattga caatttccag cccaaccatc aactgaccgt ctttaagtgt
     3241 gggtggcggg taatatttga tgggtttgcc agctatggta tatatcgggt tgtccagttg
     3301 gagatggatg tatcctccta cattgtcata actggaataa gtgtgagtaa caacattatc
     3361 tatggcatag ataatgttgt tctcttcaag atccgggttg agtgtttcat gggttatctt
     3421 gtattctttg tcttccagtc tcagatactc gatgttgagg ttggtgtcta ttatgtaggc
     3481 gctacctttt gacaacattt tgtacttgtg catattttca aagctgacaa accccgatga
     3541 accggtacca tgtttagaaa atgaactttg tgtcaatgct gtgtaaaatt cataatcacc
     3601 ataattgtaa aaaacaatag ataccgctag tatgtcagac tcagtctcca gctgaactgc
     3661 tgcaattgaa acattgagag ctttagcaac ttcataacaa taatcagggt cttttggact
     3721 cagcttgaca gggttgttgt ttttgtccag aactggcaag taggcaaatg attttgcagt
     3781 ttgctttttc tccaacctgt agaagaccag gccttcctct tttgtgtgct ccattatggt
     3841 atatttttta tccagatctt caaatgagta gtctttgtct actactattt tgaagtccgg
     3901 gaactgcgaa ggggcagcct ctttgttcag ggactttgca tgccccaatg gggacaccag
     3961 gattattgat gcaagcaaaa atattattgt tttcttcatg gttcatcctc tcttttcgtg
     4021 tatttaaaaa agactcattt ccttgttttt catctgcttg ttgtcattca ttcattggag
     4081 cgaaaaatct ggcgacaatg gccgaatagt cagtttttct gtagtggcag ttggccttga
     4141 ggagcttgca aacatcaaga agtgggacaa aaagcccagt gtctgtcatg aatggaggtt
     4201 cggataactc aacctctttg ccatcaataa gtgctttttt actgtccttg gtcaatgtca
     4261 gctttaccag ctcaacctcc gtggttttga tattatatag cctttggatt gtatatgttt
     4321 caccatcacc cgtcattggc gcaaagagca catcggaaag aaagcttaaa gcaacgaacg
     4381 gtatttggtt ttcagacaac acaagtgcaa aattagtgta ggaatgctca acagtattaa
     4441 ttactacttt tggatctttt gcactgagcc agagatatcc ggcaccactt tgacgattta
     4501 tatatgatgc ctcaccaaaa tccacaaggc ctggaatttc gtaacggtca tagtattcag
     4561 gattgagtgc ttcccttcgt tctacctggt atgcataccc atgatatgcc tgcacctcaa
     4621 agttgtaatc cccatcaagg tagcttattg aaccgactgg atatatcttg tctgttcccg
     4681 agatatattg acttctattg atgtcctttc caaaaatgca gtatttccat ttataatagt
     4741 tgtcatcctc ttggaagaac attcccaaag caatccggcc ctgtgggcca tcaagcttgc
     4801 caaaaagccg cctactggta attatctttt cagctttttt cacaatgtcc ttgtctgccg
     4861 gatcaacctt gaccggtgat ccatattcat ccagaacggg tgaatagtgt ttcccagtct
     4921 cgtcttctac aatttcaaat tcataaaatg tgagtttgcc tttttcatac gaaagcattg
     4981 gcgtgggttt tttgaaagac cagaatgaac caccagaaaa tgacctatac tcatatgcga
     5041 ttatcgtaaa agggtcctgt ggccagatca ttttctgaat gccaaaactg gggctggaat
     5101 atggggtgac aattattgca aagacaacta gaaccagaac aaacttcctt agattgagaa
     5161 ttacattcat tttgccctcc atcgactcaa ttggtttcca atacaagaaa cggaccaaga
     5221 ggcaaaacgg ttcgaagaaa tttttgttga aacacttatc tgtattacat tatggtcctc
     5281 agtcacgcat tggggcaaaa aatctggcga taaggactgc cccgtcagta ccacgaatat
     5341 gacaattggc cttaaggaat ttgcatgcat cagcaacggg aacatata
//
LOCUS       DAOSRI010000111         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14142_length_5310_cov_3.675737, whole genome shotgun sequence.
ACCESSION   DAOSRI010000111 DAOSRI010000000
VERSION     DAOSRI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14142_length_5310_cov_3.675737"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK717_04295"
     CDS             complement(<1..440)
                     /locus_tag="PK717_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOR46915.1"
                     /translation="MEELTVAWRSEGYLEEKKSEFFSFLVPSKSLAEFEGFISELRQK
                     NREMCHFCSAVRLRNPTLVEKASDDGEPSGTAGRPILNVLQRKNLINCGIVVMRKFGG
                     TLLGTGGLTRAYSEAAQSCVLSAKMGRMAPFSLMNGTIGYREWA"
     gene            complement(450..1265)
                     /locus_tag="PK717_04300"
     CDS             complement(450..1265)
                     /locus_tag="PK717_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOR46916.1"
                     /translation="MLSDSGVVAQTGLFEKIASYFNSFNFLITLAILAFFVFLIIASR
                     KYKWDVHTIAIVGICSGLSLVFGYFPFFRMPEGGTISLAMVPVVVVALTKGVVPGVIT
                     GVIAGCLQYFPDPLFYNLPQFLLDYPVAWGLVGLAGLWKTGGEKKTLLWVIGGLVLLV
                     CGGFILDLVLEGGYSWDKFVALAIMLGLLVPGFLIAKRGGAWSPAGGVAIGAFARFLA
                     HLLSGMLFFYTFAWPGYSILGYTSIYIAIHLVPETILCMVAAVPINKYVIERS"
     regulatory      complement(1293..1394)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1516..2262)
                     /locus_tag="PK717_04305"
     CDS             complement(1516..2262)
                     /locus_tag="PK717_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46917.1"
                     /translation="MNERDERIEELAEHMVKTQGLDKEAARKIATEMFEYRPAGPSWF
                     SKSLKKTVSVSPMIIGAMGVAIIGLSVALVTTNIGKNQNEYLVSSIGTVTTTLPDVSA
                     NNAFNFKINDAIPPTGMFNYEAVPMPEQPNTIVVPSSEKMEGDTTVTYKIEENGEEST
                     YFVYENTNPAPHIMKGDTTVTYHIKDSKEGGVYSFETPPVLKFKIESNKLLPNPNTPP
                     ITITPSTGAKSSSDEPPTITITPKTGTKAR"
     gene            complement(2286..2900)
                     /locus_tag="PK717_04310"
     CDS             complement(2286..2900)
                     /locus_tag="PK717_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR46918.1"
                     /translation="MSMTNESMTGESTNRKQQKENTRNNILQAALREFVDKGLADTNV
                     QDVAKAANVSYGTIFAHFPKKDDLLVRVINEYGSQIVEKFNNLVKEENDLEKILRADL
                     KVIGEYEPFYAQLVSSISRLPSDARNVLIGIQSVVSHHFQRGLKKGVTNWIFKDLPGH
                     LAFNCWTSLIYYYLSNRDLFAPGGSVIENKGEELIGFYMRMVRK"
     gene            3050..4231
                     /locus_tag="PK717_04315"
     CDS             3050..4231
                     /locus_tag="PK717_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46919.1"
                     /translation="MRRCISVVLLLLCLFLASFPASAENKTVSLDKEHVYNGHVNSRF
                     CVWRNAAKGNSSVLNLFDMANGKQFKINSGIMLEMAQYQEEIHLSSAYVCWSSIGPGW
                     PKYGMACTGTTPKKLCENSTIDKRANIYDSRVVFFSTDNGRGIYWNDIADMESKPVLI
                     DKLAERTAYYYEKFPRIANGKVVYCRPNEESGLEEVCLYDTETGELQVIDSAKEVVMP
                     NITREFIIYGVMDKGGNPELRALNLETNEKFTFFDKTLAKMTISIPNNPYSELFLFKT
                     RTKTRYDSVDKLMCYDPITKKTITIAEPSSNGDKPKDAFYLESLSGCCCGRRVAYCEK
                     IGLNSQSGKLFVVDVKKDGSLKKTDIVSDYAEIGIFRVFCDKMIYTIRNKGLYLTQLG
                     E"
     gene            complement(4423..>5310)
                     /locus_tag="PK717_04320"
     CDS             complement(4423..>5310)
                     /locus_tag="PK717_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR46920.1"
                     /translation="DKNLYCIDSVEGSLIWKHETGGTVGSPAVSGGYVCFASQDKKLY
                     CLSSATGETVWEIEGTQNFSSPTIYQSKIYYASIDRNVYCVDLSSGQEAWRFETKGPI
                     GSSVTVVNDRAYVGSNDYNVYCLNAKNGNELWSFKTDNAVVSTPSVWGDRVFFGSYDD
                     KIYCLNATTGEKIWEFKTGGDIVSTPSVYIDRLIVGSNAGVVYCVSIEDGKELWSFDP
                     GLWVPIPSTPAIAGTKMFVGSADKNVYLIDTITGKKLWHFETSDRIFASPAVYKNRVY
                     IGSADGKLYCFEDKAATGK"
BASE COUNT         1373 a         1227 c         1224 g         1486 t
ORIGIN      
        1 ttcgcccatt ccctgtagcc aatggtcccg ttcatcaatg aaaatggtgc cattctgccc
       61 atttttgcag acaggacgca ggactgggcg gcctctgaat aggcccttgt gagaccgcct
      121 gtcccaagaa gcgttccacc aaactttctc atcacgacaa tcccacaatt tatcaggttc
      181 ttgcgttgca agacattcag tatgggtctg ccggctgttc cggaaggttc accatcgtcg
      241 cttgcctttt caacaagcgt tgggttcctg agcctgactg cactgcagaa atggcacatt
      301 tccctgtttt tctgcctgag ttccgaaatg aaaccctcaa actccgcaag cgattttgag
      361 gggacaagaa acgagaaaaa ttcggacttc ttctcctcaa ggtagccttc cgagcgccag
      421 gcaacggtca gttcttccaa atgtatcctt tacgacctct ctatcacata tttgtttatt
      481 ggtacagctg caaccataca caagatggtc tcgggaacaa ggtgtatggc tatgtaaatt
      541 gaggtgtagc ccagtatgct gtatcctggc catgcgaatg tgtagaagaa cagcatcccg
      601 gacagcaagt gggcaagaaa acgtgcaaat gcaccaatcg caacaccacc tgctggcgac
      661 catgccccac ccctctttgc gatcaggaac cctggaacga gcaacccaag cattattgcc
      721 agtgcaacaa atttgtccca ggaatacccg ccctcaagca ccaggtccag tatgaacccg
      781 ccacaaacca gcagtaccag tccgcctatt acccacagga gcgttttttt ctcgccaccc
      841 gttttccaca gcccagccag accgacaagc ccccatgcta ccggataatc aagcagaaat
      901 tgtggcagat tgtagaataa cgggtctgga aaatattgga gacatccggc aataacaccg
      961 gtgataactc cgggcacaac ccctttcgtc aatgccacaa caaccaccgg caccattgcc
     1021 agcgagattg ttccgccttc cggcatcctg aagaatggaa agtagccaaa taccagcgac
     1081 aatccggagc aaattccaac gattgcaatc gtgtggacat cccatttgta tttccgtgat
     1141 gcaataatga ggaatacaaa aaaagccaga attgccaaag tgataaggaa gttgaaggag
     1201 ttgaaatagc tggcaatttt ctcgaagagg cccgtttggg ccacaacacc agaatcacta
     1261 agcatgcaaa agcctccttt taaattatca ggagggagga aggtcccatt ccctgcgctg
     1321 gtattaccca gatcaggtct tgcgggtcgg gcttttcgcc catctcagcc ttcacagcac
     1381 cccgatggtt attaagacat ttccttgtta catccagaag ggggctgtgt caagaaagtg
     1441 tggtcaggct atttttgaga cggtcaaata aaaaagcctc tccatctaaa gatggagagg
     1501 ctggacatct tttggttatc ttgcttttgt gcctgtcttt ggggttatgg ttattgtggg
     1561 tggttcatca gagctggact ttgcgcctgt gcttggtgtt atggttatcg gaggcgtgtt
     1621 ggggtttggc aacagtttgt ttgattcaat cttgaatttg agcactgggg gtgtctcaaa
     1681 tgagtaaacg ccgccttctt tgctatcttt aatatgatat gttaccgttg tatcgccttt
     1741 catgatgtga ggtgccgggt ttgtattttc ataaacgaag taagtgctct cttcgccgtt
     1801 ttcttcgatt ttgtacgtca ctgttgtgtc gccttccatt ttttcagagg aaggaacaac
     1861 gatcgtgttt ggctgctctg gcataggtac cgcctcataa ttgaacattc cggttggagg
     1921 tatggcatca ttaattttaa aattgaatgc attgtttgcc gaaacatcgg gcaaagttgt
     1981 tgttacggtc cctatggaac tcaccaagta ttcattttgg ttcttgccga tatttgtagt
     2041 cacaagggcc acggacaatc cgatgattgc aacgcccatt gccccaatga tcattggcga
     2101 aaccgagaca gtcttcttga gcgattttga aaaccagctc gggcctgcgg gtctgtattc
     2161 aaacatttcc gttgcgatct ttcttgccgc ttccttgtca aggccctgtg tttttaccat
     2221 gtgttctgcg agttcttcta ttctttcgtc tctttcgttc atttattttc cctcactttc
     2281 cttttttact ttctgaccat acgcatgtaa aaaccaatta attcctcacc tttgttttcg
     2341 ataacagaac caccaggtgc aaagaggtcc ctgtttgaga ggtagtagta aataaggctg
     2401 gtccagcaat tgaaggccag gtgccccggc aggtccttaa aaatccagtt tgtgaccccc
     2461 ttcttcaatc ctctctggaa atggtgcgaa acaacagact ggatgcctat gaggacgttt
     2521 cttgcatcac tcgggagcct cgatattgac gagaccagtt gtgcataaaa aggctcgtat
     2581 tccccgatga ccttgaggtc ggccctcagt atcttttcaa ggtcgttctc ctcttttacg
     2641 aggttgttga atttttcgac aatctgcgag ccatactcat tgatgaccct taccagaagg
     2701 tcatcctttt tgggaaaatg ggcaaagatc gtgccgtatg aaacatttgc cgctttggcg
     2761 acgtcctgga cgtttgtgtc ggcaaggcct ttgtccacaa attccctcaa tgcagcctgg
     2821 agaatgttgt tcctcgtgtt ctctttctgc tgcttcctgt tcgttgactc accagtcatt
     2881 gattcattag tcattgacat gtgagtcatc ataatcgtca attttgattt gtcaagtttt
     2941 ttaaggaacg ccctgtttgc ttactattgt tttaagtttc acggcattgt ttttaaaata
     3001 tggaactctt agaacaaaat cattgaaaac tggtggagga gggaatttga tgaggagatg
     3061 catatctgtt gttttgcttt tgttgtgcct gtttctggcg tcatttcctg ccagtgctga
     3121 aaacaagaca gtgtcattgg acaaagagca tgtctacaat ggtcatgtca attcaagatt
     3181 ttgtgtctgg agaaacgcag cgaagggcaa ttcgtcagta ttgaacttgt ttgacatggc
     3241 aaatggcaaa cagttcaaga tcaatagtgg catcatgctt gagatggcac aataccagga
     3301 agaaattcat ttgagttcgg cgtatgtttg ttggagctca attggtccag gctggccaaa
     3361 atatggaatg gcgtgcactg gcaccacacc aaagaagctt tgcgagaaca gtaccattga
     3421 taaacgtgca aatatttatg acagcagggt ggttttcttt tccactgaca atgggcgtgg
     3481 catttactgg aatgatattg ctgatatgga atccaaacct gtgcttatag ataaattagc
     3541 tgaacgaaca gcctattatt acgagaagtt cccccgcatt gccaatggga aggttgttta
     3601 ttgcaggcca aatgaggaat cgggtctgga agaggtttgc ctttatgaca ccgagacagg
     3661 ggaattgcag gtaatagatt ctgcaaaaga agttgtcatg cccaatatca ccagagaatt
     3721 catcatctat ggcgtcatgg acaaaggagg caaccccgaa ctgagggccc tgaacctgga
     3781 aacgaatgag aaattcacat tctttgacaa aactctggca aaaatgacca tttctatacc
     3841 aaacaaccct tattcagaac tgtttctttt caaaaccagg acgaaaaccc gctatgattc
     3901 agttgacaaa cttatgtgct atgacccgat tacaaagaag acaattacaa ttgcggagcc
     3961 ttcatcaaat ggcgataagc caaaggatgc tttttatctt gaaagccttt ccgggtgctg
     4021 ttgcggaaga agggtcgcat attgtgaaaa gataggcctc aacagccaaa gtggcaagct
     4081 gtttgttgtt gatgtcaaga aagatggctc cctgaaaaag actgacatcg tgtccgatta
     4141 cgctgaaatt ggaattttca gggttttttg cgacaaaatg atttatacaa tcaggaacaa
     4201 gggcctgtat ttaacacagc tgggagaatg acaaaactgg aaaaacaaaa gccccggggt
     4261 cggcggggcc ttttttagga gaggaaaaaa gttttaactt gctttgggat cggggaggtc
     4321 attcccgata caacccttac aaacgatttt cgggttttgt tcccaaaaaa caaaaagccc
     4381 tgctaaaagc agggcttttt gaatatcggt caatttttta ggctatttgc cggttgccgc
     4441 cttgtcctcg aagcagtaga gttttccatc tgcagagcca atataaaccc tgtttttgta
     4501 tacggcaggg gaagcaaata tcctgtcgct agtttcgaaa tgccagagtt ttttgcctgt
     4561 gatcgtgtct atcaggtaaa cgttcttgtc tgccgaacca acaaacattt ttgtgcctgc
     4621 gatcgctggc gttgacggga ttggcaccca aaggccagga tcaaatgacc agagctcttt
     4681 gccgtcttct atcgaaacac agtaaacgac tcctgcgttt gaaccgacaa taagcctgtc
     4741 aatgtagact gaaggcgtcg agacgatgtc tccgccagtc ttgaattccc atattttctc
     4801 gccagttgtt gcattcaggc agtatatttt gtcgtcgtag gaaccaaaaa acactctgtc
     4861 cccccagacg gatggcgttg aaacgacggc gttgtctgtc ttgaaagacc acagctcgtt
     4921 cccgttcttg gcgttcagac aatacacgtt gtagtcattc gagccgacgt atgccctgtc
     4981 atttaccact gtgaccgagg agcctatcgg accttttgtt tcaaagcgcc atgcttcctg
     5041 gcctgacgac aggtcaacgc agtaaacatt gcggtctatt gaagcgtagt agatctttga
     5101 ctggtagatt gtgggtgatg aaaagttctg ggtgccttca atttcccaga ctgtttcacc
     5161 tgtggcagag ctgagacagt aaagcttttt gtcctggctt gcaaagcaga catacccgcc
     5221 agaaactgcc ggtgaaccaa cggttccgcc agtttcatgc ttccatatca gcgaaccctc
     5281 aacactgtcg atgcaataaa ggtttttgtc
//
LOCUS       DAOSRI010000112         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14235_length_5287_cov_3.181957, whole genome shotgun sequence.
ACCESSION   DAOSRI010000112 DAOSRI010000000
VERSION     DAOSRI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_5287_cov_3.181957"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="PK717_04325"
     CDS             <1..148
                     /locus_tag="PK717_04325"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOR46921.1"
                     /translation="LGPDEFYVMGDNRGGSLDSRTFGPIKRDSILGRAFIVFWPAIRL
                     QVLQ"
     gene            195..758
                     /gene="lepB"
                     /locus_tag="PK717_04330"
     CDS             195..758
                     /gene="lepB"
                     /locus_tag="PK717_04330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR46922.1"
                     /translation="MRINKIRLFQDPAARREIASWVLAISLALSFSMATRAYAFEPRR
                     VQQTSMYPTLHDGDIVFLYKLDHIIRRGDIIVFNSPTSNEKLIKRVIGLPGETISFEN
                     GNLLINSTRFVENSDRTETDYNQPVGNSPIATPSGTKLGEDEYFVMGDNRDVSYDSRS
                     FGPIKRSSILGKAEAVLWPVDRISTLK"
     gene            815..1423
                     /locus_tag="PK717_04335"
     CDS             815..1423
                     /locus_tag="PK717_04335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOR46923.1"
                     /translation="MKIHSGCEVGSSGCYEKNKHLIIGVDEAGRGPLAGPVVACACHI
                     TEHIDDVADSKKLTAKKRHQLASEIILKGQVCIGIENSRSIDVSDILKATMAAMKTAA
                     NTLCKKLGGKGLVIVDGNRVPDGLVWQSKAIIGADDSQYCVSCASIVAKVIRDHLMDG
                     FDMTFPGYGFSKNKGYGTADHLEAISKLGPCQIHRRSFGPCK"
     gene            1414..2139
                     /locus_tag="PK717_04340"
     CDS             1414..2139
                     /locus_tag="PK717_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOR46924.1"
                     /translation="MQIINKSDYVKRKIKLEYLKIISGLLLVVAGFFGLALGFVLNWL
                     FFIIGFLLFVFGGMILSNRTGEVSIWKAGFVGQQMVPKALSMLPNEFVLINNVSIKDK
                     NCDIDHVLIGPKCLYAIESKNYNGEIYGQGDEWGYMKRGRAGGFYKGHIGNPAKQIKR
                     SVWELKQMLDEKLLKMSFDPNQFWIEPIVVFTNPNTILKVTDSTVKAMLLKDLPSYIQ
                     ECDAKTQIPEDMLKTITDILRNE"
     gene            2132..2482
                     /locus_tag="PK717_04345"
     CDS             2132..2482
                     /locus_tag="PK717_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOR46925.1"
                     /translation="MSRSSTLGKKGEEMAALLLASKGFEIVCKNYKTKYGEIDIICKK
                     DENLLFVEVKRRSANIFGTPLESLPAKRVRRMADAVMAFLSENPEFQESQIQLALVGI
                     SNGKTHIVPVDCNM"
     gene            2466..3374
                     /locus_tag="PK717_04350"
     CDS             2466..3374
                     /locus_tag="PK717_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46926.1"
                     /translation="MIAICSLASCSSYSQRAAPEGEFVKPELYGDECFDLGNSWVECN
                     LIGDGVVRQATFIDTGKESFFLVKNGPQGPIKRTIGPKGPLDALIIRPGLFDLTGDRV
                     PEIIWPVKNDQLTGLSVVSLAGGPEKTLFEHPRINFATTGGTDYSPVLFLSLSDPTLG
                     IMAIPWRVHGEKMLPDPFGSDRPFSTEITNQKKSAVGKAWRDYFDGIKPKLETSGLEG
                     ELLESAKTALAFYYWDMGQNAEATSLIIGDEENNPDLSKLLFNKGFWALWRGNIGLAK
                     TFFLSSMTQIGGNKISDCLEILKSLP"
     gene            complement(3616..4536)
                     /locus_tag="PK717_04355"
     CDS             complement(3616..4536)
                     /locus_tag="PK717_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46927.1"
                     /translation="MKKLDSLHQPPFNTCLVGAVKGALDYFDCNVSVAATFGGSGHAF
                     LVNIHEQLCPSGPYCWKYEEFYPLVRNLGLEMVELGFCHNGSTPEERMAMEQKVREYL
                     DQGQPCAVGNMDNQLIYSYDDNKFLMAQPWDACCETTPPSLTFETWKEFGEEIHAVFW
                     TFRKTPAKDESSIIRDSLRYALDLFQNPEKHSFEHFATGLAAYDNWAKAVEAGYGASH
                     GSWWNAMVWSECRAMAGAWFEELAAKHNNISSQAHKLSGMYKEIAANLEKISNKEMDS
                     GEKIRIIRDVKAMEQEAIINIKDLLTVFDR"
     gene            4858..5184
                     /locus_tag="PK717_04360"
     CDS             4858..5184
                     /locus_tag="PK717_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46928.1"
                     /translation="MTDKEILEAIGSAEEKAKTELENAKLGAQELISSAKKRAKEIED
                     KARLDAIEKGKKDLEAVDIEVNSIVMESVNGSKKKSEELKASLGKNLPKAAEALSREA
                     LEKWPF"
BASE COUNT         1534 a         1215 c         1257 g         1281 t
ORIGIN      
        1 tcttggcccg gatgaatttt atgtcatggg cgacaacagg ggcggctccc tcgattccag
       61 gacattcggc ccgataaagc gggactctat ccttgggaga gcttttattg ttttctggcc
      121 agccataaga ctccaggtac tgcaatagga actcctgggg tcacttgtct gtaaggactg
      181 gcaggaggta ccttgtgaga ataaataaaa taagactttt tcaagaccca gcggcaagaa
      241 gagagattgc ctcctgggtc ttagctattt cattggccct ttcattttca atggcaacaa
      301 gggcatacgc atttgaacca agaagggtcc agcagacttc aatgtaccca accctgcatg
      361 atggtgacat tgtcttcctt tacaaacttg accacataat aagaagaggg gatataattg
      421 ttttcaattc tccaacctca aacgaaaaac tgatcaagag ggtgattggc ctgccaggag
      481 agacaatcag ttttgagaac ggcaacctgc tcatcaacag caccaggttc gtggaaaata
      541 gcgacagaac cgaaaccgac tacaaccagc cagttggcaa ttcaccgata gcaacaccct
      601 ccggaacaaa gctaggtgag gacgaatact tcgtgatggg cgacaacagg gacgtttcct
      661 acgactcgag gtctttcggg ccaataaaac ggagctcaat ccttggcaag gctgaggcag
      721 tgctatggcc tgttgacaga atcagcacac tgaaatagcg aaaaacttgc aaaaaacaca
      781 aaaagcccta tcttgaagga tagggctttt taaattgaag attcattcgg ggtgtgaagt
      841 gggatcatct ggttgttatg aaaaaaacaa acacttgatt attggggttg acgaagcggg
      901 gaggggaccg cttgctggtc cagttgtggc ctgtgcttgc catataacag aacacataga
      961 tgatgttgca gactcaaaaa aactgacagc caaaaaaagg caccagcttg catcagaaat
     1021 aatcctgaaa gggcaagtct gcataggcat cgaaaacagc aggtccatcg atgtgtcaga
     1081 catactgaag gcaaccatgg cggcaatgaa gacggccgca aacaccctct gcaaaaaact
     1141 tggaggaaag gggttggtta tagttgatgg caatcgtgtt cccgatgggc ttgtgtggca
     1201 gagcaaagca attattgggg ccgatgacag ccagtattgt gtttcctgcg cctcgatagt
     1261 ggcaaaagtc ataagagatc atcttatgga cggttttgac atgacatttc ccggatacgg
     1321 cttttccaaa aacaagggat acgggacagc tgaccacctg gaagccattt cgaagctggg
     1381 accctgccaa atccatagaa ggagttttgg cccatgcaaa taataaataa aagcgattac
     1441 gtaaagagaa aaataaagct tgaataccta aaaatcattt ctggattatt acttgttgtg
     1501 gcaggttttt ttgggctcgc ccttggtttt gtccttaatt ggttgttctt cataatcggt
     1561 ttccttcttt ttgtttttgg cggaatgata ctcagtaaca ggacaggtga agtgtccatc
     1621 tggaaggccg gttttgtcgg ccagcagatg gtgccaaaag cgctttccat gctcccgaac
     1681 gaatttgtgc tgatcaacaa tgtttcgatc aaagacaaaa attgcgatat tgatcacgta
     1741 cttattggcc caaaatgcct ctacgcaata gagagcaaga actataacgg tgagatctat
     1801 ggccagggcg acgaatgggg ctacatgaag agggggaggg ccggcggctt ctacaaaggg
     1861 cacatcggca atcctgcaaa gcagataaaa cggtcagttt gggagctaaa acagatgctt
     1921 gatgaaaaac ttttaaaaat gtcgtttgac ccaaaccagt tctggatcga gccaatagtt
     1981 gtcttcacaa atcccaacac aatcttgaag gtgaccgact cgactgtaaa ggccatgctt
     2041 ctgaaggact tgccctcata cattcaagaa tgcgatgcaa aaacacagat tccggaagac
     2101 atgctgaaaa ccattaccga catactccgc aatgagtagg agctctaccc tcggcaaaaa
     2161 gggtgaagaa atggcagcat tgctgcttgc atcaaagggt tttgaaatag tttgcaaaaa
     2221 ctacaaaaca aaatatggcg agatagacat catctgcaaa aaagatgaaa acttgctgtt
     2281 tgttgaagta aagagaagat ctgcaaacat ttttgggaca ccgctcgaat cccttccggc
     2341 aaagagggtg aggcgaatgg ctgatgcggt aatggcattc ctgtctgaaa atcccgagtt
     2401 tcaggaatcg caaatccagc ttgcacttgt aggaatatcc aatggcaaaa cgcatattgt
     2461 gcctgttgat tgcaatatgt agtcttgctt cctgctccag ctattcacag agggcagcac
     2521 cagaaggtga gtttgtaaaa ccggagctgt atggcgatga gtgttttgat ctcggcaata
     2581 gctgggttga atgcaatctg ataggcgatg gtgttgtcag gcaggccaca ttcatcgata
     2641 cagggaaaga atcttttttc ctggtcaaaa atggcccgca aggccccatc aaaaggacga
     2701 tcgggccaaa aggcccgctt gacgctctga taattagacc cggactcttt gatctgaccg
     2761 gcgacagggt cccggaaatc atctggcctg tcaaaaatga tcaactcaca gggctttcgg
     2821 tggtttcgct ggctggcggc ccggagaaga cactgttcga gcacccaagg ataaactttg
     2881 ctacaactgg aggaacagac tacagtcctg ttctcttcct gtccctatcc gacccaacgc
     2941 ttggcataat ggcaataccc tggagagtgc acggggagaa aatgcttccg gacccatttg
     3001 gaagtgacag acccttttca accgaaataa caaaccagaa gaaatcggcc gtcggaaaag
     3061 catggagaga ttattttgat ggcataaagc caaaactgga aacctcaggt cttgagggtg
     3121 agttgctgga aagtgcaaaa accgcactgg cattttatta ttgggacatg gggcaaaatg
     3181 ctgaagccac aagtctcata atcggagacg aggaaaacaa tccagatttg tcaaaacttc
     3241 tttttaacaa aggtttctgg gcgctgtgga ggggcaatat tggtcttgcc aaaacatttt
     3301 tcctgtcctc aatgacacag atcgggggca acaaaatcag tgattgcctt gaaatcctca
     3361 aatccttgcc ttagcacgaa gaagggaaaa cagaattagg ccaatggcaa ccagaatgtt
     3421 tgcaatacta atttgttgcc gggccagggc ttcatccatc cgacttgaac gcctcaatgt
     3481 tagtactccc aacatatctt gcctcgaaac ccggctccag caattcttgc gggaccaact
     3541 cgtcttttac tcttaagccc atctttatct cctgcatact tggataactg gccctaataa
     3601 ccaatctcag tacaactacc ggtcaaatac tgtgaggaga tctttaatat tgatgattgc
     3661 ttcctgttcc atggccttca catcacggat aatgcgaatt ttttcgcccg aatccatttc
     3721 cttgttgctg atcttctcaa gattggccgc gatctctttg tacattccac tcagcttgtg
     3781 ggcttgtgac gagatattgt tatgcttggc agcaagctct tcgaaccagg cgccggccat
     3841 tgcccggcat tccgaccaga ccatagcgtt ccaccaactg ccatgactgg ctccataacc
     3901 agcttcaacg gctttagccc agttgtcata agctgccagt ccagttgcaa agtgctcaaa
     3961 gctatgcttc tcagggttct ggaatagatc tagcgcatag cgcaagctat cgcggatgat
     4021 gcttgattcg tctttggctg gcgtctttcg gaacgtccag aacacagcat ggatctcctc
     4081 gccaaattcc ttccaggttt cgaatgtcag gcttggcggc gtcgtttcac agcaggcatc
     4141 ccacggttgg gccataagga atttgttatc gtcgtaacta taaatgagct gattgtccat
     4201 gttgccaaca gcacagggct gcccctggtc caaatactcc cgcactttct gttccatggc
     4261 catgcgctcc tccggtgtgc tgccattgtg gcagaaccct agttcaacca tctcaagccc
     4321 taggttgcgc accaatgggt agaactcttc atatttccag caatatggcc cacttgggca
     4381 aagttgctca tgaatattga caagaaacgc gtgtccagag ccaccaaagg tggctgcaac
     4441 acttacattg caatcaaaat aatcaagtgc gcccttaacg gcgccaacca ggcaagtgtt
     4501 gaatggtggt tgatgaagac tgtcaagttt tttcattttt acacctctcc tttttgcaat
     4561 gcggcatcac acaggccatt tttggacctg caagtcaccc ttttgggcgg gattgtggca
     4621 ttagtatcac cttgtgtcaa gacaagggtt gcccatagag tgtgtggcaa accgtctgtg
     4681 caatatacct tggatttaac gtttatttat cgatatggga accagcagtt tccggcatta
     4741 cgagccctgc aaaaacacct ttcttgcttt ttcatacgca aacggggaca aaaccaggaa
     4801 acatattgaa attaccaaat ccttgcctta gcaaatcata aagctatcat aaataatgtg
     4861 accgacaaag aaatccttga agcaattggc agtgcagaag aaaaagcaaa aacagaactg
     4921 gaaaatgcaa aactgggtgc ccaggaactc atttcttctg caaaaaaaag ggcaaaagag
     4981 atagaggaca aggccaggct cgatgcaatc gagaaaggca aaaaagacct cgaagctgtg
     5041 gatattgaag taaactctat tgtcatggag tccgtaaatg gcagcaaaaa gaaatcagag
     5101 gagctaaagg catcgctcgg gaaaaacctg ccaaaagctg cggaagcact ctcaagggag
     5161 gcgcttgaaa aatggccatt ctaaggatgg ccaaagccat cctcggcaca ccacccgaac
     5221 acctcgaaca gacactggcc ttgttgcagg aatttggcgc cctcagcaca agaccttttg
     5281 aaggcct
//
LOCUS       DAOSRI010000113         5282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14256_length_5282_cov_4.420892, whole genome shotgun sequence.
ACCESSION   DAOSRI010000113 DAOSRI010000000
VERSION     DAOSRI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14256_length_5282_cov_4.420892"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /gene="murA"
                     /locus_tag="PK717_04365"
     CDS             <1..1091
                     /gene="murA"
                     /locus_tag="PK717_04365"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOR46929.1"
                     /translation="PGTWSLSSNGDLKSVVEGAQTEKIRGSQTLLGAILARKGEVTLQ
                     ALGGCRIGARPIDLHIKGLKALGAEVEMVKGSVFAKVNGRLKGANIFLDFPSVGATEN
                     IMLAASTADGQTIIENAAAEPEIEDLAKFLRLCGAKISGDGTKTIQIEGVESLKGTHH
                     SIIPDRIETGTFMIGAVMAGGDVLIEQALPSTVDSLIFKLQEAGVIVRIENHTSIRIR
                     NTSRPKAINIKTLPYPGFPTDLQPQTTAMCSIADGVSVISESVFESRFGAVPELVRMG
                     AEIQAEGHSVIVKGVEKLQGTEVIAPDLRGAVALVLAGLCAEGTTKVVGAHHADRGYE
                     KFEMKLKGIGADIERVTENQSNQHPEEQ"
     gene            1088..2305
                     /locus_tag="PK717_04370"
     CDS             1088..2305
                     /locus_tag="PK717_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR46930.1"
                     /translation="MIDFEYTSEQNMVAKMVRELAAREFAPHIKEWDEKAIFDPGVLP
                     KLAEQGILGLSTPEKYGGAGFDYVSLGLACEELEYVDTALRVILSVHIGLVTLPILTW
                     GNEKQRTKYLPDLASGKRIGAFGLTEPNAGSDVVALETKAEKDGDGWLISGEKMWISL
                     ADVADTFLVFAWTDQDKRKKRDHSGISAFVIERGMPGITTASIHGKLGVRAGNTGSIS
                     MDRVKVGPEHVLGEVGEGFKIAMFCLDQGRFTVGWGATGLVKACLDASVSYALSRVTF
                     GVPIGHHQLIKEMIGNMKVNYEASRLLNLQAAWMKNKGLRCTKETAQAKWFACNASEK
                     SASDCVQIHGAYGFSNEFPAERFYRNAKGAVIYEGAREIQALMAADYALGFRTDKPTR
                     KSLPPHPYPENWQ"
     gene            2536..4230
                     /gene="kdpA"
                     /locus_tag="PK717_04375"
     CDS             2536..4230
                     /gene="kdpA"
                     /locus_tag="PK717_04375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOR46931.1"
                     /translation="MVAGELMIVALIVLATIACAYPFGIYMAKVFGGEKTFMSKVMRP
                     VENFFYRFAGIKENDEQNWKQYLKSFVLFFGLGFVVLFTILSIQKWLPMNPSGFGNIR
                     WDTGLNIATSFCTNTNWQSVGFESSLTPFSQMVGIAVQNFVSASAGLAVGIALVRGFA
                     RKNTDKIGNFWVDLTRGTLYVLVPVSLVMAIFLIWQGVPQTLGNTIQTTTIQGQSQTI
                     VTGPVASQVSIKMLGSNGGGYFNANGAHPFENPTPLSNAIQTMAILFIPMSLLFTFGK
                     MIGDMKKAWSLFFVCMFLFLIGFSITMYAEMSPNPVLRNAGATTLNMEGKEMRFGIPS
                     SALWGQATTVVSNGSVNGMHDSFMPLSGLVYIFNMITGEVIFGGVGVGLAGLLLYSIL
                     AMFIAGLMIGKTPELLRKKLGVNEMVWACVGLLTPCIVVLILTATGFVTPNAVSSLSA
                     KGSHGFSQLLYANASMVGNNGSAFGGLSANTPFFNLTGSFAMLFGRFATVISAIAVAG
                     SLARKNQSPANATQIPMTSPAFVVMLLAVIIIVGALTFFPSLAIGPLLEHLKMTGM"
     gene            4239..>5282
                     /locus_tag="PK717_04380"
     CDS             4239..>5282
                     /locus_tag="PK717_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOR46932.1"
                     /translation="MAKKSLFTGDIVKTAILGSFVRLNPARLWRNPVMMIVFAVSAIV
                     TVQMVYLAATRQPFGFELQISIWLWITVLFANFAEALAEGRGNAQAQSLKTNRNNTAA
                     NKLEHDGSIKVVPATTLEKDDIVIVAEGEIIPSDGDIVHGVALIDESAITGESAPVVR
                     ESGGDRCGVTGGTRVLSGKLEIRITAGQGETFIDHMISMVESAKRMKTPNEIALEILL
                     IALTSVFLCVAITLPVFSNFFGTQISVVILASLLVCLMPTTIGGLLPAIGIAGMDRLL
                     SKNVIALSGRAVEASGDVDVVLLDKTGTITLGNRQAVELIPVDGIDERHLFRCALLSS
                     LADDTPEGRSILSL"
BASE COUNT         1514 a         1146 c         1266 g         1356 t
ORIGIN      
        1 cacccggaac atggtctctc tcatcaaatg gtgacctgaa atcggttgtc gagggtgctc
       61 aaacagagaa aataagaggc agccagaccc ttttgggcgc catactggcc agaaaaggtg
      121 aagtgaccct ccaggcactc ggcgggtgca ggataggcgc aaggccaatt gatcttcaca
      181 tcaaaggcct gaaagctctt ggtgccgaag tggaaatggt aaagggctca gttttcgcaa
      241 aagtaaacgg aaggcttaaa ggggcaaata tatttcttga ttttccctcg gtaggtgcaa
      301 cagaaaacat aatgcttgcg gcatcaacag cagatgggca aacaataata gaaaatgccg
      361 ctgcagagcc ggagattgaa gacttggcaa aattcctcag gctgtgcggt gcaaaaatat
      421 caggcgatgg aacaaaaaca atccagattg agggtgttga gtcattgaag ggtacccacc
      481 attcaataat accggacagg atagaaaccg ggactttcat gattggagca gtaatggctg
      541 gtggagatgt tctcatcgag caagcactgc catcaactgt cgattcctta attttcaagc
      601 tccaggaggc aggggtaatc gtaagaattg aaaaccacac atcaataagg atcagaaaca
      661 cgtccaggcc taaagctata aacataaaaa cattgccata tccaggcttt ccaaccgacc
      721 tacaaccaca gacaaccgca atgtgctcga ttgctgatgg tgtttcagtg atttccgaat
      781 cagtttttga aagcaggttt ggcgcagttc ctgaacttgt aagaatgggt gccgagatac
      841 aggcagaagg tcattcggtc attgtcaaag gtgttgaaaa actccagggg accgaggtga
      901 ttgctcccga tttaagaggt gccgttgcac ttgttctggc aggactctgt gcagaaggaa
      961 caacaaaagt tgttggggca caccacgccg acaggggata tgaaaagttc gagatgaagc
     1021 taaagggaat cggcgccgat atcgaaaggg tgaccgaaaa ccaatctaat cagcatccgg
     1081 aggaacaatg atagatttcg aatacacttc agagcaaaac atggtggcaa agatggtcag
     1141 agagcttgcg gcaagagaat ttgcaccgca cattaaggaa tgggacgaaa aagcaatttt
     1201 cgatcctgga gttttaccaa aactggccga gcaaggcatt ttgggactct ccactcccga
     1261 aaaatatggt ggggcaggtt ttgattatgt ttcattgggg cttgcctgcg aggaactgga
     1321 atatgtagac acagctcttc gcgtcattct ctccgtgcat atcggactgg tcacactccc
     1381 aatacttact tggggcaatg aaaaacaaag gacaaaatat ctgccagacc tcgcttccgg
     1441 aaaaagaata ggtgcatttg ggctcacaga accaaacgcc ggttcggatg ttgttgcgct
     1501 tgaaacaaaa gccgaaaaag atggtgatgg ttggcttatt tccggcgaga agatgtggat
     1561 ttcccttgca gatgttgctg atactttcct agtctttgca tggactgacc aggacaaaag
     1621 aaaaaagaga gaccactccg gcatttcagc ttttgttatt gaaagaggaa tgccaggcat
     1681 cacaactgcc tcaatccacg gcaagctcgg cgtcagggct gggaacaccg gctcgatctc
     1741 gatggacagg gtcaaggttg gaccagagca tgtcctcggc gaagttggtg aaggattcaa
     1801 gatagccatg ttctgcctcg atcagggcag atttactgtc ggttggggtg caaccggcct
     1861 cgttaaagcg tgccttgatg cttctgtctc ctatgccctg tccagggtca cttttggagt
     1921 gccaatcggc caccatcaat taataaaaga gatgatcggg aatatgaagg taaactacga
     1981 ggcaagcaga ttgctgaatc tgcaggccgc atggatgaag aacaagggct tgaggtgcac
     2041 aaaagaaact gcgcaggcaa aatggtttgc ctgcaacgca tcagaaaaat cggcctcaga
     2101 ttgcgtccag atccacggtg catacggctt ctccaatgaa tttccggcag agaggttcta
     2161 caggaatgca aaaggcgctg tcatttatga aggcgcaagg gaaatccagg cgcttatggc
     2221 cgcagattat gctcttggtt tcaggacaga caaaccaacc agaaaatcac tcccaccaca
     2281 cccataccca gaaaactggc agtaagaacc aataatacag cccccgtttc ttcacagaat
     2341 tcatagaagc gggggatttt tttgaaaacc tttttataga attcctcaaa aacatttcaa
     2401 aaaatctttg ctatttcaaa gaaaaactat tccaataatt ccaatttgtg aattctatta
     2461 acaagaaagt gattgatttc caattttaaa gtatagaatc aattccttcg aacaattgta
     2521 taaaggagat gaaaaatggt tgctggtgaa ttgatgattg ttgcactcat tgttcttgca
     2581 acaattgcat gtgcctatcc atttggcatt tatatggcca aagtttttgg tggcgaaaaa
     2641 acttttatgt caaaggtcat gaggcctgta gaaaatttct tttacagatt tgccggcatc
     2701 aaagaaaatg atgaacaaaa ctggaaacaa tatctgaaat cttttgttct attctttggt
     2761 ttgggttttg ttgttctttt tacaattctt tccatacaaa aatggctacc aatgaacccg
     2821 tctggttttg gcaacatcag atgggacact ggcctgaata tcgcaacatc attttgcaca
     2881 aacacaaatt ggcagtctgt cggttttgaa tcttctctaa caccattttc acaaatggtc
     2941 ggaattgcag tccagaactt tgtttcagcc tctgccggtc ttgccgttgg gattgcgctt
     3001 gtcagaggct ttgcaagaaa aaacacggat aaaataggca atttctgggt ggaccttaca
     3061 cgaggcacac tctatgttct tgttccagta tcgcttgtca tggcaatttt cctgatctgg
     3121 cagggtgttc ctcaaacatt gggcaataca atacagacaa caacaataca ggggcaatct
     3181 caaaccattg tcacaggtcc agttgcatcc caggtgtcca taaaaatgct tggaagcaat
     3241 ggtggtggat acttcaatgc aaacggggca catccatttg aaaacccaac accactttca
     3301 aatgcgatac aaaccatggc aattctcttt ataccgatgt cacttctgtt cacattcggg
     3361 aaaatgattg gcgacatgaa gaaggcctgg tcactattct ttgtgtgcat gtttctcttt
     3421 ttaataggtt tctccataac catgtacgca gaaatgtccc cgaatccagt tttaaggaat
     3481 gctggcgcaa ccacactaaa tatggaagga aaggaaatgc gctttgggat tccatcttca
     3541 gcgctctggg gtcaagccac aacggttgta tcaaacggct cagttaatgg aatgcatgac
     3601 agtttcatgc cattgtcagg gttagtgtac attttcaaca tgataacagg agaggtaatt
     3661 tttggtggag taggcgtagg ccttgccggt ctccttcttt attccattct ggcaatgttt
     3721 attgctggtc tgatgattgg caaaactcct gaattgctcc gcaaaaaact tggagtcaat
     3781 gaaatggtgt gggcatgtgt tggtttgctg actccatgta ttgttgttct gatacttact
     3841 gcaacaggtt tcgtgacacc aaatgctgta tcgagcttgt ctgccaaagg ttcacatggt
     3901 ttctcacagc tcctttatgc taacgcatcg atggtgggca acaacggatc ggcctttggc
     3961 ggtctttctg ccaacacgcc atttttcaac ctgaccggct catttgcaat gctttttggc
     4021 aggtttgcaa ctgtaatttc agcaattgcc gtcgctggtt ctcttgccag aaaaaaccag
     4081 tccccggcga atgcaaccca gattccgatg acaagtccgg catttgtagt gatgctgctt
     4141 gccgtgataa taattgttgg tgcactcact ttcttcccgt cgcttgcaat aggaccactt
     4201 cttgagcacc tcaagatgac aggaatgtga ggcagaaaat ggcaaaaaag agtctattta
     4261 ccggagatat tgtaaaaaca gccattttgg gctcgtttgt ccgcctcaat cctgcaagat
     4321 tatggcgcaa tccggtcatg atgatagttt ttgcggtctc ggcaatagta acagttcaaa
     4381 tggtttacct tgcagcaaca aggcaaccat tcggttttga attgcaaata tcaatatggc
     4441 tatggatcac tgtactgttc gccaattttg cagaggcatt ggccgaaggc aggggcaacg
     4501 cacaggccca aagcttgaaa acaaacagga acaacacggc tgcaaacaaa ctcgaacatg
     4561 acggttccat caaggtagtc ccggcaacaa cactcgaaaa ggacgacata gtcatcgttg
     4621 ccgagggtga gatcattccc tctgacggag acattgttca tggcgtagca ttgattgacg
     4681 aatcagcaat aacaggagag tctgcacctg tggtcagaga atcaggtgga gacagatgtg
     4741 gtgtcacagg tggaactaga gtgctttctg gaaagttgga gatcagaata acggctggcc
     4801 agggtgaaac atttattgac cacatgattt cgatggttga gagcgccaaa aggatgaaaa
     4861 caccaaatga aatcgccctg gaaatactat tgattgcgct cacatcagtc tttctatgcg
     4921 tagcaattac ccttccagta ttttcaaatt tttttggcac acaaatatcg gtggtcattc
     4981 ttgcaagcct acttgtgtgc ctgatgccga caacaatagg cggtttgctg cccgccatcg
     5041 gaatagctgg catggacagg ctcctctcca aaaacgtcat tgcgctgtct ggaagagctg
     5101 ttgaggcatc cggcgatgtt gatgtagtcc tgttggacaa aacgggcaca ataacacttg
     5161 gaaacaggca agctgttgag ttgataccag ttgatggcat cgatgaacgc catctgttca
     5221 ggtgtgcgct cctttcatcc cttgccgatg acacgccaga aggcagatca atcctctctc
     5281 tt
//
LOCUS       DAOSRI010000114         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14292_length_5270_cov_3.651007, whole genome shotgun sequence.
ACCESSION   DAOSRI010000114 DAOSRI010000000
VERSION     DAOSRI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14292_length_5270_cov_3.651007"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1563)
                     /gene="hydG"
                     /locus_tag="PK717_04385"
     CDS             complement(175..1563)
                     /gene="hydG"
                     /locus_tag="PK717_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOR46933.1"
                     /translation="MEAGFIREPEIWKTLEESKRPSREAIKETFSKALDLKGLDYKDI
                     AILSSISDPELLAELFGTAKKAKETIYGKRLVLFAPLYISNLCTNECLYCAFRAKNTE
                     LKRRVLTQEEIAHETRQLIDQGHKRILLVTGESYPKQGFKYVLDSITTIYAQKSGKSG
                     EIRRVNVNVAPLTLEEFRDLKAAKIGTYQLFQETYNHETYHAVHLAGKKSDYDWRVTA
                     MDRAMEAGIDDVGIGVLFGLSDWRFELMAILQHIRHLEMKFGVGPHTISVPRLEPASG
                     SDMSSHPPKPVSDIDFRKIVAILRLAVPYTGIIMSTRETPNIRREAFALGVSQISAGS
                     RTNPGGYCEDEEFDSSQFSLGDHRSLDEVVRDIAMLGYIPSFCTGCYRLGRTGQDFMD
                     LAKPGEIKKHCDPNALSTFTEYLEDYASNETREVGDKLIKETLEGMGEREKIVSQSLI
                     EKVKTGKRDCYI"
     gene            complement(1575..2717)
                     /locus_tag="PK717_04390"
     CDS             complement(1575..2717)
                     /locus_tag="PK717_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR46934.1"
                     /translation="MPEILKVLVVDDELGIQKGCQRVLTEYKFTLPDVEDEISFEVAT
                     TGSGEEALEILEKNPQDIVLLDYKLPGMTGLDVLSIMAEKKIETMAIMITAYASLETA
                     VVATKQGAFDFLAKPFTPDELRSVIKKATKNLILQRQAKKLAEEKRQVRFQFISVVAH
                     ELKAPLAAIEGYLNIMNEGLVTDPTAQHQMVERCLLRLTGMRKMVTDLLDFTRIESGN
                     KVREFVGLDLVEIARQSMETMSSSASARGIKIELVAPETACMTADRGEIEIIFNNLVS
                     NAVKYNRDGGRIIITIAQIPGGFRISVADTGIGMTEEEVGKLFGEFVRIKNVKTKNIL
                     GTGLGLSIVKKLALLYGRDVNVESHVDVGSTFNVILMEAQGPQQAS"
     gene            complement(2718..3110)
                     /locus_tag="PK717_04395"
     CDS             complement(2718..3110)
                     /locus_tag="PK717_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR46935.1"
                     /translation="MPNSKKKILVVDDDVDYLFQQRVQLESAGFEVKTAETQADGEKM
                     LAEEKPDLVIVDLMMENMDAGFSLAYHIKSKYPGIPVIMVTGVTSETGLEFSAGGGNS
                     WIKADALLAKPIRFEQLLGEINKLLKGK"
     gene            complement(3122..4033)
                     /locus_tag="PK717_04400"
     CDS             complement(3122..4033)
                     /locus_tag="PK717_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR46936.1"
                     /translation="MSGQSWREWVKKSESDFLNCGACGYDTCLDHAMSIYRGLAEDEM
                     CLPYTIEKLKQTNEELEKSNSKLSDTQQQLVQSERLASMAQMATGIAHEVNNPLGVVL
                     MYAHMLKDQCKDDKQLGEDLGMIVEQADRCKKIVAGLLNFARQNQVAFESVDLNEIVE
                     KGLQIVERPKNIAAEIINQMENPYCELDKDQMVQVLVNLISNAYAAMPDGGKLTITTG
                     GNDFQVWFSIADTGIGIPKENMPKLFQPFFTTKKMGKGTGLGLAVTYGIVKMHRGDIK
                     VTSNADLSKGPTGTTFTVAIPRAGRRE"
     gene            complement(3993..5132)
                     /locus_tag="PK717_04405"
     CDS             complement(3993..5132)
                     /locus_tag="PK717_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOR46937.1"
                     /translation="MKRKEHSAFVSTNKERCRVCYTCVRECPAKAIRISDGQAELLAE
                     RCISCGNCVRVCSQDAKELVRTKDEVEKIISENQKVAAIVAPSFAAEFYDMKYDRFVG
                     MLRALGFSFVNEVAFGADLVSREYRKLLLSTKEGRWIATTCPAIVTYIEKYSPDLVPN
                     LAPIVSPMVATARALKRLHGQDLKIVFIGPCIAKKVEASSSLIEGEIDGAITFLELRE
                     MFDEHGIKSDAVKPTDFDPPHANIGMVYPISGGMLQTAKIGEDLISGEAVRAEGRTRF
                     LEAIKEFENGQLDARLLEVLCCEGCIMGAGMTTKEQEFKRRSRISKYVRDCLKYRNQG
                     QWEKDMVYFADLDLRRSFERDDKRVGIITPDIDLRPILERMGKKI"
     gene            complement(5145..>5270)
                     /locus_tag="PK717_04410"
     CDS             complement(5145..>5270)
                     /locus_tag="PK717_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOR46938.1"
                     /translation="IRTSHANKSVKRLYDEFLGEPNSHKAHELLHTHYHKRDVLK"
BASE COUNT         1224 a         1332 c         1145 g         1569 t
ORIGIN      
        1 agcacacttt gtcatcaact tcatgaaggc caatccttgt acggatgttg cacccgtaat
       61 cggtcaatat tttttgaacc tggcctgcat ttttcgccct gttagtgacc tgtatcccaa
      121 acaccattct cttgttcatt gctacctcct tgggacatat tctacacaaa gagtttagat
      181 ataacagtcc ctcttgccag tttttacctt ttcaatgaga gactgagaaa cgattttctc
      241 tctttcaccc attccttcaa gcgtttcttt gataagttta tcgccaactt ctctggtttc
      301 attcgaggca tagtcttcaa ggtattcggt gaaggttgaa agagcgtttg ggtcgcaatg
      361 tttctttatc tcgcccggct ttgcaaggtc catgaagtcc tggccagttc tgccaagcct
      421 gtagcaaccg gtgcaaaatg acgggatata gccaagcatt gcaatgtccc tgacaacctc
      481 atcgagagac ctgtggtcgc caagcgagaa ctgtgagctg tcaaactctt catcttcaca
      541 atacccgcca gggttcgtcc tggaacctgc ggagatctgc gaaaccccaa gcgcaaacgc
      601 ctctctcctg atgtttggcg tttcccttgt agacatgatt ataccggtat atggaacagc
      661 aagcctcagg attgccacaa tcttcctgaa atcaatgtca gaaacaggtt ttggagggtg
      721 cgatgacatg tcagatcccg acgctggctc gagtctcggt acgctgatcg tgtgagggcc
      781 aacaccaaac ttcatttcca ggtgtctgat gtgctggagg atggccatga gttcaaaccg
      841 ccagtccgag aggccaaaca aaacaccaat gccaacatcg tcaatcccgg cttccatggc
      901 cctgtccatt gcggtgacac gccagtcata gtccgatttt ttgcctgcaa gatggacggc
      961 atggtatgtc tcgtggttgt aggtttcctg gaaaagttgg tatgttccta tctttgcggc
     1021 cttgaggtcg cggaactctt ccagcgtgag tggcgcaaca ttgacattga ctctcctgat
     1081 ttcaccactt tttccagatt tttgggcata aatggtcgtt attgaatcaa gtacatactt
     1141 gaaaccctgt ttgggatatg attcaccagt aacaaggagt atcctcttgt gcccctggtc
     1201 aatgagctgc cttgtctcat gggctatctc ttcttgtgtc agaacacgtc tcttgagttc
     1261 agtgtttttt gccctgaaag cacaataaag acactcattt gtgcaaagat tggagatata
     1321 gagcggagca aacaggacaa gcctcttgcc gtaaatggtc tcttttgcct tctttgcagt
     1381 gccgaaaagt tcggcaagca attctggatc ggaaatcgac gagaggatgg ctatgtcctt
     1441 gtaatccaat cccttcaaat caagtgcttt tgaaaacgtt tccttgatag cttccctgct
     1501 tgggcgtttt gattcttcaa gtgttttcca tatttctggt tccctaatga agccagcttc
     1561 cataaaaacc tcctttatga agcctgctgt ggcccctggg cttccatgag gataacgttg
     1621 aatgtagagc caacatcaac atggctctcg acgttaacat ctcttccata gagcagtgca
     1681 agctttttga cgatggaaag gccaagacct gtgccaagga tgttttttgt cttgacattc
     1741 tttattctta caaattcccc aaaaagcttg cccacttctt cttcagtcat gcctattccg
     1801 gtgtcagcca cagagattct gaatccacca ggaatttgcg caattgttat gatgattcta
     1861 ccaccgtccc tgttgtattt gacggcattt gaaacaaggt tattgaagat tatctcgatt
     1921 tcaccacgat cggctgtcat gcaggcagtc tctggtgcca caagttcaat ctttatgcct
     1981 cttgcagaag cactggaaga cattgtttcc atggattgcc tggctatctc gacaaggtcg
     2041 agaccaacaa actccctgac cttgttgcca gattcgatcc ttgtgaaatc taaaaggtcg
     2101 gtgaccattt ttctcatgcc agtgagcctg aggagacatc tctcgaccat ctggtgttgc
     2161 gcggtaggat cagtgacaag cccttcattc atgatgttga ggtaaccttc tattgccgca
     2221 agcggtgcct tgagctcatg tgcaaccacc gagatgaact ggaacctgac ctgcctcttc
     2281 tcctcggcca gcttttttgc ctggcgctgg aggataaggt ttttggtggc ttttttgata
     2341 acactcctca actcgtctgg cgtaaacggc ttcgcgagga aatcgaatgc cccctgcttg
     2401 gttgccacaa cggcagtttc aagcgaggca taggcggtta tcattattgc catcgtttca
     2461 atcttctttt cggccattat cgagagcacg tctaggccgg tcattcctgg aagtttgtaa
     2521 tcaagaagaa ctatgtcttg tggatttttt tcaagaatct caagtgcttc ctcaccactt
     2581 ccggtcgttg caacttcaaa agaaatttca tcttccacat ccgggagggt aaatttatat
     2641 tcggtgagga ctctttgaca tcccttctgg atgccaagct cgtcatcaac tacaaggact
     2701 ttcaggattt cgggcattta ttttcccttt agaagcttgt tgatctcccc aaggagctgt
     2761 tcaaacctga ttggtttggc aagcagtgcg tcagccttga tccaggagtt gccaccgcca
     2821 gccgaaaatt ccagccccgt ttcagaagta acacctgtga ccatgatgac cggaatgccg
     2881 ggatatttgg attttatgtg gtatgcaaga gaaaatcctg catccatatt ctccatcatg
     2941 aggtcaacga tgacgagatc cggtttctcc tctgcgagca tcttttcacc atcggcctgg
     3001 gtttcggcag tcttgacctc aaaacctgca gattcaagct gcactctttg ctggaataaa
     3061 taatcaacgt cgtcatcaac aaccagtatt ttcttctttg aatttggcat gttttctcct
     3121 tctattccct tcgacctgct cttggtattg cgaccgtaaa tgttgttccg gtcggtccct
     3181 tggacaagtc agcgttagat gtgactttta tatctcccct gtgcatcttg acgatgccat
     3241 aggtgaccgc aaggccaagg cccgttccct tgcccatctt tttggttgtg aagaaaggct
     3301 ggaaaagctt tggcatgttt tctttcggga taccaatccc tgtatctgca atcgagaacc
     3361 agacctgaaa atcatttcca ccagttgtta tggtcagctt tccaccgtcc ggcattgccg
     3421 cgtaggcatt ggagatgagg tttacaagca cctgtaccat ctggtctttg tcaagctcac
     3481 agtacggatt ttccatctga ttgattattt ctgctgctat attctttggt ctctccacaa
     3541 tttgcagacc cttttcaaca atttcgttaa gatcgacaga ctcaaatgca acctgattct
     3601 gcctagcaaa attgagtaat cctgcgacaa tctttttgca cctgtctgcc tgttcaacaa
     3661 tcatgcccaa atcttcaccc agctgtttgt catccttgca ctggtccttg agcatgtggg
     3721 catacatgag cactacgcca agggggttat ttacttcatg ggcaatgcct gtagccatct
     3781 gggccattga agcaaggcgt tctgactgga caagctgttg ttgggtatca gagagtttgc
     3841 tgtttgattt ttccagttct tcatttgttt gcttcaattt ctcaatggta tatggcaggc
     3901 acatctcgtc ctcggcaagc cctctgtaga tagacattgc atggtcaaga caggtatcat
     3961 aaccacaagc accacagttt aggaaatcgc tttcagattt ttttacccat tctctccagg
     4021 attggcctga gatcgatgtc tggtgttatg ataccaactc tcttgtcatc acgttcaaaa
     4081 gacctcctca gatcaagatc ggcaaaatag accatgtcct tttcccattg cccctggttc
     4141 ctgtatttga ggcaatccct aacgtacttg cttatcctgg agcgcctctt gaattcctgc
     4201 tcctttgtgg tcattccagc acccattatg caaccttcac aacagagtac ttcaaggagc
     4261 ctcgcatcaa gctggccgtt ctcaaattct tttatggcct ccaaaaaacg cgttctgccc
     4321 tcagccctta cagcttctcc agagatgagg tcttcaccaa tctttgccgt ctggagcatt
     4381 ccaccagaga ttgggtaaac cattccgata tttgcgtgtg gcggatcaaa atctgttggc
     4441 tttacggcat cagatttgat tccatgctcg tcaaacatct ctctgagctc cagaaatgtt
     4501 atcgcaccat caatttcccc ttcaataaga cttgaggagg cctctacctt ctttgctatg
     4561 catggcccga taaaaacaat tttcaggtct tggccatgga gcctcttgag cgctctagca
     4621 gtggcaacca ttggtgacac aatcggggcc agattgggca cgaggtctgg actgtatttt
     4681 tctatataag ttactattgc gggacatgtt gtcgctatcc agcggccctc ttttgtagac
     4741 aaaaggagtt ttctgtattc ccttgaaacc agatcggcac caaaagccac ctcattgacg
     4801 aacgaaaaac cgagcgccct gagcatgcca acaaacctgt cgtatttcat gtcgtaaaac
     4861 tcggcggcaa aacttggcgc gactatggca gcgactttct ggttttcaga gattatcttc
     4921 tctacctcgt cttttgtcct gacgagctcc ttcgcatcct ggctgcaaac cctgacacaa
     4981 ttgccacagc tgatacacct ttcggcaagc agctcggcct ggccatcaga aatcctgatg
     5041 gccttggccg ggcattccct gacacaggtg tagcatacgc ggcaacgctc tttattggtc
     5101 gagacaaaag cggagtgctc tttgcgtttc atatcagctc tattttactt taaaacatcc
     5161 ctcttgtggt agtgggtgtg caatagttcg tgggctttgt gcgagtttgg ttcgccaagg
     5221 aactcatcat agagtctctt tacagacttg ttcgcatgtg aagtcctgat
//
LOCUS       DAOSRI010000115         5239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14412_length_5239_cov_5.052855, whole genome shotgun sequence.
ACCESSION   DAOSRI010000115 DAOSRI010000000
VERSION     DAOSRI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14412_length_5239_cov_5.052855"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..807)
                     /locus_tag="PK717_04415"
     CDS             complement(58..807)
                     /locus_tag="PK717_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46939.1"
                     /translation="MEEPKETENSFDKNSAIEESQKQKQDQQFYELVSSLNRIFNPPT
                     ITIPGSLVSAIRATFKSCYPIGSMPTNEALKDICETINKIRNYNMNPVLARMIDENRK
                     ALFLNASRNLLEEEPKYRAEKDVFNNFVKDKEKEYLKKEKEKEYEYSEGVILIADCLG
                     TKKSWEKNNIQGYVKKIYDTRSKLEAVLKSRSDYINSKSKYEKYSDFFEIGFSCCFLS
                     DTFVGIMKQTKKHNKKERHYALDKPMQLATI"
     gene            complement(822..914)
                     /locus_tag="PK717_04420"
     tRNA            complement(822..914)
                     /locus_tag="PK717_04420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Ser,seq:cga)
     gene            complement(988..1785)
                     /locus_tag="PK717_04425"
     CDS             complement(988..1785)
                     /locus_tag="PK717_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR46940.1"
                     /translation="MTFQNPRKYGVPPFNVVVVHGGPGACGEMAPMAREFSSLFGVLE
                     PLQTVTTIDGQVKELEEAIKTHGSPPVALVGHSWGAWLAFIFAAKNPLLVGKLVLVGS
                     GPFEEKYASEIMPARLARLPENERNELAGLLKRMENQDEEDGDAIMDRVGDLISKSDH
                     FDPLPFGPEPVEFRPEVYQGVWPEASRLRQNGELLEMGKKITCPVVAIHGDHDPHPAD
                     GVRVPLEKTLKNFKFVLLEKCGHDPWLEKHAREKFFAVLKGELGSKI"
     gene            complement(1782..2720)
                     /locus_tag="PK717_04430"
     CDS             complement(1782..2720)
                     /locus_tag="PK717_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR46941.1"
                     /translation="MNHTFISDFQKGQEIDDYYAVAKKTKRSTKNGSYFISLTLSDRT
                     GRVDAVVWNATLTKFGEDYNENQVVRVCGRVDEYDGKIQIVVEKIGIGDATKYEASWF
                     LPQSPVPVDDVMKQIFSLVASVKDEKLRLLGESFLGSEDYSPNFRVAPGAVYLHHAYL
                     GGLAAHTLAVMKFCDSLCQLYPLNRDLLIVAALFHDVGKMVENTWELIFKRTIEGELV
                     GHLIISRDILREFTKSTGIAPEVALHLEHCILSHHGQYLYGSPKLPMTREAFALHVAD
                     LADSRFEEFDRELKNIAPGESTEPIRYLDGIRLFNL"
     gene            complement(2717..3175)
                     /locus_tag="PK717_04435"
     CDS             complement(2717..3175)
                     /locus_tag="PK717_04435"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOR46942.1"
                     /translation="MTTLPHLMQLAFDQALVAFEKGEVPVGAVVAKGSEILGLGHNLT
                     LTLKDPTAHAEIMAIRDATSKLGDWRLSDCQIYVTLEPCTMCVGAILLSRIETINFLA
                     YDPKDGACGSTKNLAVQPVFGHCAKVIKIDDGGRSSDLIREFFRLRRENK"
     gene            complement(3186..3258)
                     /locus_tag="PK717_04440"
     tRNA            complement(3186..3258)
                     /locus_tag="PK717_04440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3223..3225),aa:Arg,seq:acg)
     gene            complement(3261..4718)
                     /gene="gltX"
                     /locus_tag="PK717_04445"
     CDS             complement(3261..4718)
                     /gene="gltX"
                     /locus_tag="PK717_04445"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR46943.1"
                     /translation="MTEKIRVRIAPSPTGKLHIGNARTGLFNYLFARHYGGTFILRID
                     DTDTERSTPEAEKAIFDGLKWLGITWDEGPDIGGNYGPYKQTERFDTYKEALDRLLED
                     GHAYYCFCSPEEIEQKRTTAEKAHEIYRYDNTCRTIPLDEARKRIAAGERAVIRLRVP
                     EGTEANFHDLIRGDIEVDSETFDDFVIAKPGGAPLYNFATVIDDHLMDITHILRGQDH
                     LTNTSKQILIYRALGWDVPKFGHFSLILDTNRKKLSKRAGAVYIGDFQEQGYLPQTVA
                     NFIALIGWSPGENKEKMTMEEMVGAFSMDHVQKADAVFDPEKLKWLNGVYIREMPLEN
                     LVEAVKPHLRNAGFEVDNVDQEWLKKVVFLEQERMRLFSEASELFDFFFKEIPYDKSL
                     LSSKKHSDDELARALEQTREQLEGISSWDKEELENLCRSLASQMGWQGGDLFMPLRIA
                     VTGKKATPPLFETMEVLGKELSLKRIDKAIQSLKK"
     gene            complement(4728..4820)
                     /locus_tag="PK717_04450"
     tRNA            complement(4728..4820)
                     /locus_tag="PK717_04450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4784..4786),aa:Ser,seq:gct)
BASE COUNT         1298 a         1262 c         1176 g         1503 t
ORIGIN      
        1 cgaggtaggt tctggcctct tcttcggtaa gttgtccaag ttctatcagg ttcatgatta
       61 tatggtagca agttgcattg gtttgtcaag tgcataatgc cgttcttttt tattatgttt
      121 tttggtttgc ttcataatgc cgacaaatgt gtctgagagg aaacagcatg aaaatcctat
      181 ttcaaaaaaa tcgctatatt tttcatattt tgatttgcta ttaatataat ctgaacgact
      241 ttttaatact gcctcaagtt tcgaacgggt atcgtagatt ttcttaacat atccttgaat
      301 gttatttttt tcccaagatt tttttgtacc caagcaatca gcgattaaaa taacaccttc
      361 ggaatattca tattcctttt ctttttcttt ctttaaatac tccttctcct tatctttgac
      421 aaagttatta aaaacatctt tttccgcacg atattttggc tcttcttcaa gaaggttcct
      481 ggatgcattt aaaaataaag cttttcgatt ctcatcaatc attctagcca atactgggtt
      541 catattatag tttctaattt tatttattgt ttcgcaaata tcctttagtg cctcatttgt
      601 aggcatagaa ccaattgggt aacaagattt aaaagtagct cttatcgcag ataccaacga
      661 tcctgggata gttatagttg gtgggttgaa aatcctattc agggaactaa caagctcata
      721 aaattgttga tcttgttttt gtttttgcga ctcttctata gcactattct tatcaaaaga
      781 gttttctgtt tctttcggtt cttccattct ccctccttgt ctggcggaga gggggggatt
      841 cgaacccccg aagcagggat tgcccactta cctgatttcg agtcaggcgc tttcaaccgc
      901 tcagccacct ctccacaaac catgaaaatt ctaaatgtct gggtttcaaa atcaaggtgg
      961 aggtttcaca gcctcctgtt gcttggccta gattttactg cccaactccc cttttaatac
     1021 tgcaaagaac ttttcccttg cgtgcttttc cagccacggg tcatgcccgc acttctccag
     1081 caggacaaat ttgaagtttt ttaatgtctt ttcaagcggg accctcacgc catcggccgg
     1141 gtgcgggtcg tggtcgccgt ggattgccac caccgggcag gtgatttttt tgcccatttc
     1201 cagcagttcc ccgttttgcc tgagacgtga cgcctccggc cacacaccct gatagacctc
     1261 cggcctgaat tccaccggct ccggcccaaa aggcagcggg tcgaaatggt ctgactttga
     1321 gataaggtcg ccgaccctgt ccattattgc atcgccgtcc tcttcgtcct ggttttccat
     1381 ccttttcagg agcccggcaa gctcgttgcg ctcgttttca ggtagcctcg ccagtcgtgc
     1441 cggcattatt tctgaagcat acttttcctc aaacggcccg ctcccaacaa ggacaagttt
     1501 cccaaccagc aaagggtttt ttgcggcaaa gatgaaggcc agccatgcac cccacgagtg
     1561 gccaacaagc gccaccggcg gactgccatg ggtttttatt gcctcttcaa gctccttgac
     1621 ctggccatct atggttgtta cggtctggag cggttccagc acgccaaaaa gggatgaaaa
     1681 ctcccttgcc ataggcgcca tttcaccaca ggcccccggg ccgccgtgga caacaacgac
     1741 attaaaaggc gggacgccat attttctggg gttctgaaag gtcacaggtt gaaaagcctg
     1801 atgccatcca gatatcttat tggctcggtg gattcgccgg gtgcaatgtt tttcaactct
     1861 ctgtcaaact cttcaaatct tgagtcagca aggtctgcaa cgtgtaatgc aaaagcctcc
     1921 ctcgtcatcg ggagttttgg tgaaccatac aagtactggc cgtggtgcga gaggatgcag
     1981 tgctcaaggt ggagcgcgac ttctggtgct atccccgtag actttgtgaa ttccctgagg
     2041 atgtcgcggc tgattatgag gtggccgaca agctcgccct cgattgtcct tttgaagatg
     2101 agctcccatg tgttttcaac catcttgccg acatcatgaa atagcgcagc aacaatgagt
     2161 agatcgcgat ttagcggata gagctggcac agggagtcgc aaaacttcat tacagcaagc
     2221 gtgtgggcgg caaggccccc aagataggca tggtgcagat agacagcacc aggggccacc
     2281 ctgaaattgg gagaataatc ttcactgcca aggaacgatt ccccaaggag cctgagtttt
     2341 tcgtccttaa cagacgccac cagagaaaaa atctgcttca tcacatcatc aacaggaacg
     2401 ggtgactggg gaaggaacca cgaggcctcg tatttcgtcg catcacctat gcctattttt
     2461 tcaacaacga tctggatttt gccgtcgtac tcgtcaaccc ttccacacac cctgacaacc
     2521 tggttttcat tgtaatcttc accaaatttt gtcagcgtgg cgttccagac gacggcgtcg
     2581 accctgccgg tgcggtccga gagtgtcagc gaaataaaat agctaccgtt ttttgtggac
     2641 cttttggttt tcttggcgac agcatagtaa tcatcaatct cctggccttt ttgaaaatcg
     2701 ctgatgaatg tgtggttcat ttgttttccc tcctgagtct gaaaaattcc ctgatgaggt
     2761 ccgaggaacg cccgccatca tcaattttta tgacctttgc gcaatgcccg aatactggct
     2821 ggacggcaag attttttgtg cttccgcaag caccatcctt tggatcatag gccaaaaaat
     2881 tgatggtttc gattcttgag agaaggatcg ccccaacgca catggtgcaa ggctcaagtg
     2941 tgacgtaaat ctggcaatca gagaggcgcc agtcaccaag ttttgaggtt gcgtccctta
     3001 ttgccattat ttctgcatgg gctgtagggt ctttaagtgt gagggtcagg ttgtggccaa
     3061 gaccaaggat ttcactgcct tttgccacca ctgcaccaac aggcacctcg cctttttcaa
     3121 aagcaacaag ggcctggtca aatgcaagtt gcataaggtg cggcagcgtc gtcatgaatg
     3181 acaagcgcgg ctgacaggac ttgaacctgc aacctacggc tccgtaggcc gttgctctat
     3241 ccgttgagct acagccgcac ttacttttta agcgactgta tggctttgtc aatgcgtttt
     3301 aagctaagtt ctttgccgag gacttccatg gtctcaaaaa gtggcggtgt tgccttcttt
     3361 cctgtgaccg caatcctgag cggcatgaaa aggtcgccac cctgccaacc catctgggag
     3421 gcaagcgacc tgcaaaggtt ctcaagttct tctttgtccc agcttgatat tccttcaagc
     3481 tgttccctgg tttgctcaag tgctctcgca agttcgtcat cggagtgctt ttttgaagaa
     3541 agcagcgatt tgtcgtatgg tatttccttg aaaaagaaat caaacagctc cgaggcctcg
     3601 gagaacagcc tcatcctctc ctgttcaagg aagacaactt ttttgagcca ttcctggtca
     3661 acgttgtcga cctcaaaacc agcatttcta agatggggct tgaccgcttc gacaagattt
     3721 tcaagcggca tctcccttat atagacacca ttaagccatt ttagcttttc aggatcaaag
     3781 acggcatcgg ctttttgaac gtggtccatg gagaaagctc caaccatctc ttccatggtc
     3841 attttttcct tgttttcacc cggtgaccaa ccgattaggg caataaagtt agcgactgtc
     3901 tgtggaagat agccctgttc ctgaaaatcg ccaatataga ctgctccagc tctcttcgag
     3961 agttttttgc ggttggtgtc caatatgagg gagaagtgtc caaactttgg aacgtcccag
     4021 ccaagggccc tgtaaataag gatctgtttt gatgtgtttg tgagatggtc ctgtcctctg
     4081 aggatgtggg ttatgtccat caggtggtca tcaatgactg ttgcaaagtt gtaaagcggt
     4141 gccccgccag gttttgcaat gacaaaatca tcgaaagtct cggaatcaac ctcgatgtcg
     4201 cccctaatga gatcatggaa attggcctct gtaccctctg gaacgcggag ccttattacg
     4261 gccctttcac cagcagcgat cctttttctt gcttcatcaa gaggaatagt gcggcaagtg
     4321 ttgtcgtacc tgtatatctc gtgtgccttc tcggctgtag tacgtttttg ctctatctct
     4381 tccggggaac aaaaacaata gtaagcatgg ccatcttcaa ggagcctgtc cagtgcttcc
     4441 ttgtatgtgt caaatctttc agtctgtttg tacgggccat aatttccgcc aatatccgga
     4501 ccctcatccc atgtgattcc aagccactta aggccatcaa aaatggcttt ttcggcctct
     4561 ggggtggagc gttcggtatc ggtatcatct atcctgagga taaatgttcc accatagtgc
     4621 cttgcaaaaa gatagttgaa aaggcctgtt cttgcattgc caatgtgtaa tttgccggtt
     4681 ggtgacgggg ctattctgac cctgattttc tctgtcatga ttacctctgg cggagaggac
     4741 gagattcgaa ctcgtgaacc agttttacac cagttactcg cttagcaggc gagtgccttc
     4801 aaccgctcgg ccacctctcc aattttgcat actttgtaaa ctttatcatt caaagatgcg
     4861 atgtcaacaa caaatcactc acaggcagga gtgaaaaata ccagcaaaaa acgtagatac
     4921 ctaataaaat tacgcttctt ttatcagttt ggtgattttt aaccaatctc aaatgacaaa
     4981 atataccccc atggcgtact agttgtagaa aggagatgag aacgagaaaa aataatactg
     5041 acaattttga tgaaaacgca atataagaaa tagcagacat gaataaggta aagcagaaaa
     5101 gctaaaggag gaaataatga aaaagatagt attgacaaca ctggccgttt tgatggtgat
     5161 atcagtagcg gcatacgcat acgcagcaga agaaatctgg ggaaggatca attcggttga
     5221 taccagtgcc cagacaatg
//
LOCUS       DAOSRI010000116         5221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14476_length_5221_cov_4.169377, whole genome shotgun sequence.
ACCESSION   DAOSRI010000116 DAOSRI010000000
VERSION     DAOSRI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14476_length_5221_cov_4.169377"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK717_04455"
     CDS             complement(<1..290)
                     /locus_tag="PK717_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOR46944.1"
                     /translation="MSEKIRLAIATAATCDGCDMAIIDIDGMLLDLLDIADIVFSQTY
                     VDTKYEDLEKMPDGYITASLVHGSMRDEENVHLAKLLRKKSKLMVAFGACSA"
     gene            complement(283..741)
                     /locus_tag="PK717_04460"
     CDS             complement(283..741)
                     /locus_tag="PK717_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOR46945.1"
                     /translation="MSAEKNKPLIVVLSCNWCTYAGADLAGVSRFQMPTNFRLIRTMC
                     SARIDPELVLKAFAKGADGVIVAGCHPGDCHYISGNYRERRRIKLLEMMLKQIGISPK
                     RFALEWISASEGQKFADTMRNFSAQIDRLGMSPIRTRLMEENERKEVAHV"
     gene            complement(738..3782)
                     /locus_tag="PK717_04465"
     CDS             complement(738..3782)
                     /locus_tag="PK717_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOR46946.1"
                     /translation="MTTTGKTDKTFGSVLVVGGGIGGIQTSLDLVEAGFKVYMVESSP
                     AIGGVMAQLDKTFPTNDCSMCILSPKLVSCGRNQNIEIITNAEVTHVTGRPGNFKANV
                     NVYPRYVDPKLCTGCGTCMQYCPVHCISGFNEGLAGKKAIFIEFPQAIPLVATIDRNA
                     CIGCQMCENVCLAKAVKYSDKQEERTLDVGAVILAPGFEEFRPELKSEYGYGRYPNVI
                     SSIQFERVLNASGPYQGHVLRPSDGKIPKKIAWLQCIGSRDPKAGKPYCSAVCCMYAT
                     KEAIMTREHVPDIEQTIFIMDMRAVGKGFEQYYNRAQNEYGIRYIRSRIPEVMEDPVT
                     HDLTLRYESEDGTEKTETFDLVVLSVGLQTSETVKKLADNLGLEMSEDGFAKTDYTNP
                     LSTSRSGIYVAGAFSGPKDIPETVAQAIGASAKAGADCAPGRATCLVEKEYPPQTDVL
                     GEEPRIGVFICHCGINIANTVDVVDVMDYAKTLPNVVVAEHNLYTCSQDTQKLISKSI
                     KDNNLNRVVVASCTPRTHEPLFQETLRESGLNPYLVEMANIREHCSWIHQGDPANATE
                     KARDLVRVAVAKARMLQPLARRSLPVTQSALVIGGGAAGMNAALDIARNGYPVTLIEK
                     TDELGGNLKYARYTNEVGDMQAYLKSLIFEATRNKLITIKTNTVLGDLSGSVGSFKPI
                     LKNTTTGEKEEISVGAIVVATGANQHKPKEYLYGEDPKVLTQLEFEKKLIEGNGVKNA
                     KSIVMIQCVGSRNDEHPYCSRICCSTAVANANLAKEENPNAEVYILYRDIRTYGFREH
                     YYNKAREKGVIFVRWDPDHKPEVIKHDGKLFVRVNDPVLKTTLEIPADLLILSSGVEP
                     NPDNKDIGTILKVPINDEGFFLEAHVKLRPVDFATDGVYLAGMAYHPRMLDETLAQAS
                     AAAGRALTVISKDKLLSEAAIANVNEDICDACRICLPMCPYSAINLKNIGEKQVANVD
                     EALCKGCGACAAACPSGAMEQLGFTTKQLASMIDAVLEEAVK"
     gene            complement(3793..4578)
                     /locus_tag="PK717_04470"
     CDS             complement(3793..4578)
                     /locus_tag="PK717_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOR46947.1"
                     /translation="MEQQPAKRGIFNPKIVAFVCNWCSSASGDLVGPIRAPYRGVPRI
                     LRVLCSGRVMADNVLEAMEMGSDGVFVAGCDSYECNYRDGNFIAKRRMHFAKNLLKFA
                     GFDPGRVEISLGFLQLISDPLEQFRKKILKLGPLGQAETENFTPEDVREKLMMARKVT
                     QDPEIGWMVGKENQLTEIGNTYDLKISQEEFDEMIIDKIKHKYRLYAVLGKIDKIPMT
                     IPEIAKQSNFPEPEVFNLIQELEHSGMVNEAGLSGRYFQYIQA"
     gene            complement(4587..>5221)
                     /locus_tag="PK717_04475"
     CDS             complement(4587..>5221)
                     /locus_tag="PK717_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR46948.1"
                     /translation="VGCRLCERACLKKHTPEDSEIPHEIDKQLSKQRVHVELAGTRPY
                     PLRCRHCDDAPCVTACMTGAMHFDPETGMVNVDYDKCIACWMCVMNCPYGGVLPNEDY
                     TKVLKCDRCLDVPIPACVDACPKDALVYEEVEMRDDNLCVGCGLCVEVCPFGAITMVQ
                     TERGLKARTNSELCRGCSLCSTSCPYGAIFMNYHTTDKLSDQIKEVIRSY"
BASE COUNT         1043 a         1324 c         1327 g         1527 t
ORIGIN      
        1 aatgctgaac aggcgccaaa agccaccatc agcttcgatt tcttcctgag cagttttgca
       61 aggtggacgt tttcttcatc tctcatggag ccgtgcacca gtgaagcagt gatgtatccg
      121 tcaggcatct tttcgaggtc ttcgtatttg gtgtcgacgt aggtctggga gaacactatg
      181 tcggctatgt cgagaaggtc gaggagcatc ccgtcaatgt cgattatggc catgtcgcag
      241 ccatcgcatg tggcagcagt ggcaatggca agtctaattt tttcagacat gggccacttc
      301 ctttctttca ttctcttcca tgagcctggt tcttattggc gacatcccaa gcctgtcaat
      361 ctgggcggag aagtttctca ttgtgtcggc aaatttctgg ccttcagatg cagaaatcca
      421 ttccagggcg aacctttttg gggatatgcc gatttgtttg agcatcattt caaggagttt
      481 tattcttctc ctctccctgt agttcccgga tatgtagtgg caatctccag ggtggcaccc
      541 ggccactatc acaccatcgg cgccttttgc aaaggccttc aggacaagtt ccgggtcgat
      601 tcttgcgcta cacattgttc gaatcaacct gaaatttgtc ggcatctgga accttgatac
      661 acctgcaagg tcggcaccgg cataagtgca ccagttgcat gaaagaacga caatcagggg
      721 tttgtttttt tctgcgctca ctttaccgcc tcctccaaaa ctgcatcaat catggatgca
      781 agctgttttg ttgtgaagcc gagttgctcc atggctccag atgggcaggc cgccgcacag
      841 gcgccacagc ccttgcacag tgcctcatcg acatttgcaa cctgtttctc gccaatgttc
      901 ttcaagttta ttgcgctgta tgggcacatt ggaaggcata tcctgcaggc atcgcagatg
      961 tcctcgttta cattggcaat tgctgcttcg gaaaggagct tgtcctttga tattacggtg
     1021 agcgcccttc ctgcggcggc acttgcctgg gcaagcgttt catcgagcat ccttgggtgg
     1081 taggccattc ctgcaagata gactccgtct gttgcgaagt ccaccgggcg gagcttgacg
     1141 tgggcctcga ggaagaaacc ctcgtcgttg attggtactt tgagaattgt cccgatgtcc
     1201 ttgttgtcgg gatttggttc gacaccggat gacaggatca ggagatctgc gggtatctca
     1261 agggttgtct tgagaactgg atcattcacc ctgacaaaga gcttgccatc atgtttgatg
     1321 acctctggtt tgtggtccgg gtcccatctg acaaagatta cgcccttttc ccttgctttg
     1381 ttgtaatagt gttccctgaa cccgtaggtg cggatgtccc tgtataggat ataaacttct
     1441 gcgttcgggt tttcctcttt tgcgagattg gcgtttgcca ctgccgttga acagcatata
     1501 cgggagcagt atggatgctc gtcgtttctg gagccgacgc actgtatcat tacaatggat
     1561 tttgcgttct tgacgccgtt tccctctata agttttttct caaactcaag ctgggtgagt
     1621 acttttggat cttcgccata gagatattct tttggtttgt gttggttggc gcctgttgca
     1681 acaactattg cacctacgct tatctcttct ttttctcctg tggtcgtatt tttgagaatt
     1741 ggcttgaagc tgccaactga gccggacaga tcgccaagaa cagtgttcgt cttgattgtt
     1801 atgagcttgt ttctggtggc ctcaaaaatg agtgatttga gatatgcctg catgtcgcca
     1861 acttcgttgg tgtatctggc atatttgaga tttccgccca actcgtccgt tttttcaatg
     1921 agcgtgacgg ggtagccatt tctggctatg tccagagcgg cattcattcc agccgcgcca
     1981 ccgccaatca caagggcaga ctgggttacc ggcagcgacc ttctggcgag tggctggagc
     2041 attctggcct ttgcaaccgc gactcttacg aggtctcttg ccttttctgt tgcattggcc
     2101 gggtcaccct ggtgtatcca cgagcagtgt tccctgatgt tcgccatctc aaccagatac
     2161 gggtttagcc ctgattccct cagagtttcc tggaaaagcg gctcatgggt ccttggtgtg
     2221 caggatgcca caaccaccct gttcaggttg ttgtctttta ttgatttgga aatcagtttc
     2281 tgagtgtcct gggagcacgt atagagattg tgttctgcaa ctacaacgtt tggcaatgtc
     2341 tttgcgtagt ccattacatc gacgacatcg accgtgttgg ctatgttgat gccgcaatgg
     2401 cagataaaaa ctccaattct tggctcttcg ccaaggacat ctgtttgtgg cgggtactct
     2461 ttctccacca ggcaggttgc ccttcctggt gcgcaatcgg caccagcttt ggctgaagcc
     2521 ccgattgcct gcgccacggt ctccggaatg tctttggggc cagagaaagc gccggccaca
     2581 tagattccgc tcctgcttgt cgaaagcggg tttgtatagt cggttttggc aaaaccatct
     2641 tcagacattt ccagtccgag gttgtctgca agttttttta ctgtctccga ggtctggagt
     2701 ccgactgata gcacaacgag gtcgaaggtt tcggtttttt cagtgccgtc ttcagattca
     2761 tacctgagcg tgagatcatg tgttacaggg tcttccatga cttccgggat gcgggagcgt
     2821 atgtagcgga tgccgtattc attttgtgcc ctgttgtagt actgctcaaa ccctttgccc
     2881 actgccctca tgtccatgat aaagattgtc tgttcaatgt ctgggacgtg ctctcttgtc
     2941 atgattgctt ctttggtggc gtacatgcag cagacggccg aacagtaggg ttttccggct
     3001 ttggggtccc tggagccgat gcactggagc caggcgatct ttttcgggat ttttccatca
     3061 gacggtctca gaacgtggcc ctgatatggg ccagaggcgt tcaggacgcg ctcgaactgt
     3121 atcgagctga tgacgtttgg atacctgcca tagccgtatt cagattttag ttccggcctg
     3181 aattcttcaa agccaggggc cagtatgacg gcaccgacat caagcgttct ttcctcttgc
     3241 ttgtcggagt attttacggc cttggcaagg cagacgtttt cgcacatctg gcaacctata
     3301 catgcgttcc tgtcgattgt tgccacgagt ggtatggcct gcgggaattc gatgaagatg
     3361 gcctttttgc cagccagccc ctcgttgaac cccgatatgc agtggaccgg gcaatactgc
     3421 atgcatgttc cacaaccggt gcagagtttt gggtcaacgt atcttggata aacatttacg
     3481 ttggccttga aattgccagg gcgcccagta acgtgggtca cctcggcgtt ggtgattatt
     3541 tcgatgttct ggttcctgcc gcatgagacc agttttggcg agaggatgca catcgagcag
     3601 tcgttggtcg ggaatgtctt gtccagctgg gccattacgc caccgattgc tggcgagctc
     3661 tccaccatgt agaccttgaa acccgcttcg acaaggtcaa gggatgtctg gattcctccg
     3721 atgccaccac ccacaacgag caccgaaccg aatgttttgt cagtttttcc tgttgttgtc
     3781 atggtgctcc tttcaagcct ggatgtactg gaaatatctg ccggacaacc ctgcctcgtt
     3841 caccattccg gagtgctcaa gttcctgtat gagattgaac acttcaggtt ccggaaaatt
     3901 cgactgtttt gcgatttccg gtattgtcat cggtatcttg tcaattttgc caagcacggc
     3961 gtagagacga tatttgtgct tgatcttgtc aataatcatc tcgtcaaatt cttcctggga
     4021 tatcttgaga tcgtaggtgt tgcctatttc ggtgagctgg ttttccttgc ccaccatcca
     4081 gcctatctca gggtcctggg tgacttttct tgccatcatg agcttttccc tgacatcttc
     4141 aggagtgaaa ttctcagttt ctgcctggcc aagcggccca agtttcagga ttttttttct
     4201 gaactgttca agcgggtcgc tgatgagctg gaggaaaccc agcgaaattt cgacccttcc
     4261 tgggtcaaac cctgcaaact tgagaaggtt ctttgcgaag tgcatccttc tttttgctat
     4321 gaaatttccg tccctgtagt tgcactcgta tgagtcgcac ccggccacaa aaacgccgtc
     4381 tgagcccatc tccatggctt caaggacatt gtctgccatg acccttcctg agcagagcac
     4441 ccttagtatt cttggtacgc ccctgtaagg tgcccttatt ggaccgacga ggtctcctga
     4501 ggccgatgag caccagttgc agacaaaagc gacaatcttt gggttgaata ttcctctttt
     4561 tgctggttgt tgttccattt gtccccctag tatgacctga tgacttcttt tatctggtca
     4621 gagagtttgt ctgtggtgtg gtagttcatg aatattgcac cgtaaggaca gcttgtcgaa
     4681 cagagtgagc aacccctgca aagctcagag tttgttctgg ctttcaggcc cctctctgtc
     4741 tgcaccatgg tgatggcgcc aaacgggcac acctcgacgc aaagtccaca accaacacaa
     4801 agattgtcat ccctcatctc gacttcttcg tagacaagcg catctttcgg acaggcgtct
     4861 acgcaggccg gtattggaac atcaaggcac ctgtcgcatt tgagcacctt ggtgtagtcc
     4921 tcattgggaa gcactccgcc gtatgggcag ttcatgacgc acatccagca tgcgatgcat
     4981 ttgtcgtaat cgacattgac cattcctgtt tccgggtcaa agtgcattgc gccggtcatg
     5041 caggccgtta cgcatggagc atcgtcacag tgcctgcacc ttaacgggta gggcctggtt
     5101 ccggcaagct ccacatggac cctctgtttt gagagttgct tgtctatttc gtggggtatt
     5161 tcggaatctt ctggagtgtg ttttttgagg caagcccttt cacaaagcct gcaaccaaca
     5221 c
//
LOCUS       DAOSRI010000117         5170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14645_length_5170_cov_4.756403, whole genome shotgun sequence.
ACCESSION   DAOSRI010000117 DAOSRI010000000
VERSION     DAOSRI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14645_length_5170_cov_4.756403"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PK717_04480"
     CDS             <1..1244
                     /locus_tag="PK717_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOR46949.1"
                     /translation="LVKRILAFLICGLMVLAVPANSGICIAPPVSKKDIAKPAPKETQ
                     NKIWKLGDRIVPGPIPPSLGAPEPALDIWRGEVFGKEAGLSLQLDVIKPTACRGQTVP
                     IAVYFHGGGWRSGNRYGIYNTIRVERKLLYQMGFAIASVDYRLSQQAKHPAQINDCKL
                     AIRYLRKNAKKFGIDPNRIGVWGHSAGGHLVAFLGTADEKDGLEGPGLPGISSKPQCV
                     VELYGPTDLTQPVGENGWRGITEELLGCKPKACPEKAINASPVTYADKDDPPELIIHG
                     ENDSAILYLQGELFARKLKQVGATCALIKVKNAEHQFTPYPGYLKISPPLARIRWVIA
                     AQFFRYLEPSLKGDLDVDGRVNLRDFILMCKQSGSVGAGMDGSPGPEWWNPMADLNSD
                     GLIDLKDMMLLLGIGLHTSVK"
     gene            complement(1932..2216)
                     /locus_tag="PK717_04485"
     CDS             complement(1932..2216)
                     /locus_tag="PK717_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46950.1"
                     /translation="MGRIGDLILGTYGVLMVIVTTPFLNMVALDSSGSMHSENYFFLS
                     ISLIVMYFSHFYLYKVFFGVKKGLIVFHSIAKDGLQKQSLVSFPYLSTHL"
     gene            complement(2282..2521)
                     /locus_tag="PK717_04490"
     CDS             complement(2282..2521)
                     /locus_tag="PK717_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HOR46951.1"
                     /translation="MKVMIQVSVYPLGTEKIGERVMEAIQAISGMGLETFTNPMSTTI
                     SCELEDGLEALEKMYRAIGQDGKAIMNVTVSNACG"
     gene            complement(2518..3177)
                     /locus_tag="PK717_04495"
     CDS             complement(2518..3177)
                     /locus_tag="PK717_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR46952.1"
                     /translation="MALFDSFASDYDSFFATKLGKAILLAEASAIMKAIRPQKDLECL
                     DAGCGTGIFTSLLAKSGMKVTGIDESTKMLDAASKKPELASTRLVNGKLTELPFENMA
                     FDRVLCSFALEFVDDQEQAAKETWRILRPNGIAAIVVLNPDSPWAKTREGVEVYKTAK
                     YLTFSKLQDFLPGLASISTCVHFLPADNCCFGAKEAWGNIKGSKKGAAIVASFRKTVQ
                     K"
     gene            complement(3212..>5170)
                     /locus_tag="PK717_04500"
     CDS             complement(3212..>5170)
                     /locus_tag="PK717_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46953.1"
                     /translation="LKDLKATLEEVSGVDLTDFWSVFFNSPKLVSTSFGLLRENKDGK
                     IIDTLSLKNNDSTECPTRFMVYYVDGESQSFVLSGKTKLYQLPKMATGIVFTDYNGII
                     PMDDDRVHSMSTANIISLLKWQKPLVCYEPGDGEDVMKRAEAWVQKYGGESVTSNFAG
                     STKKPVILVGPTPQLKVLGSAKLGNRMVKIQNDSIVWRGKQIMGEFDGIQVFYDEAEP
                     AKTYILDNGLGNLPENLAYAGIFTRRDKGCMLSFNLEKPGIPMATLGENILDPPWMQK
                     TVELDSFKQTFTINSKREYKAAWKGFDRDSFTFKEMTQTIKPGQNNINIELDTRGGKC
                     AFEIEHSSAFCYEKWCFGLQTASNECPLPELQAPKSSQNSDWKASFNQNLDYSYSIDG
                     AKGSDWIQGRELILSGLQKGKHELKIIFACKGGALSEIQAANFATGVTPPKVTINPGV
                     VVQKKGEIVLSGKADPDAKLSFKTPQGKELVADISQKDDGAFTATIKETETLTEIIVT
                     STDENGLSATARVTVTKYVMVTMQIGSKTATSDDGATYTLDVPPMILAGSTFVPMRFI
                     GERLSAEITWVASEKKVIYKLGKTIVEIWIGKKEAKVNGAEYKLASPPVIVSGKTLVP
                     LRFVAEALGAKVTWQGETKTIIIEYPNL"
BASE COUNT         1313 a         1208 c         1187 g         1462 t
ORIGIN      
        1 ccctcgtgaa aaggattctt gcttttttga tttgtggatt gatggtattg gctgtgccgg
       61 caaactccgg catttgtatt gctcctccgg tttcaaaaaa agatattgcc aaaccagccc
      121 caaaagaaac acagaataaa atctggaaac tcggcgatag gattgttcct ggccccatcc
      181 ctccctcgct tggcgcaccg gagccggcgc tggacatttg gcgcggagag gtgtttggca
      241 aagaggccgg cctttcgctc cagcttgatg tcataaagcc aacagcatgc aggggccaga
      301 cagtcccgat tgcagtctac ttccacggcg gtggctggcg gagcggcaac aggtatggaa
      361 tctacaacac aatcagggtc gaacgcaaac tcctgtacca gatgggtttt gcaatagcaa
      421 gcgtcgatta caggctctcc cagcaggcaa agcaccctgc acagatcaat gactgcaagc
      481 ttgcgatccg ctacctgcgc aaaaacgcaa agaaattcgg tatcgacccg aaccggattg
      541 gtgtctgggg ccattctgcc ggcggtcacc tggtcgcttt ccttggaacg gccgacgaaa
      601 aagacgggct tgagggccca ggattgcccg gaatttcaag caagccacag tgtgttgtgg
      661 aactgtacgg cccaaccgac ctcacccagc cagtgggtga aaatggctgg agaggaataa
      721 ccgaggaact gcttgggtgc aaacctaagg cctgcccgga aaaggccatc aatgcgtcgc
      781 ctgtcaccta tgccgacaag gatgacccgc cagagctgat aatccacggc gaaaatgatt
      841 cggcaattct gtaccttcag ggagagctgt tcgcaagaaa attgaagcag gtcggtgcca
      901 catgtgctct gataaaggtc aaaaacgcgg agcaccagtt cacgccgtac cctggctatc
      961 tgaaaatcag ccctccgctg gcaaggatac gctgggtgat tgccgcgcag ttcttccgct
     1021 acctggaacc ttcgctaaag ggtgacctgg atgttgatgg aagagtcaac ctgagagatt
     1081 ttatcctgat gtgcaaacag agcggctctg tgggtgccgg aatggacggc tcgcccgggc
     1141 cggagtggtg gaacccgatg gccgatttga attcagatgg gctgattgat ttgaaggata
     1201 tgatgctact gctggggatt ggtctgcata catctgtaaa ataattctgg aaaaaacaca
     1261 aaaaatacta gaaagaaaca tgcaagcata aacaatccat aaagagccaa tcccaaatca
     1321 aagaaaccta ggctataagg atggaataga ttgtctggac ttccattcat gctgtatatt
     1381 gttcccaaac tgtagttcat ctgaagaacc acaaatgagt agaaagatat tgcagtcaaa
     1441 ccggcgattg ttttccatat tatatgataa aaatgtggac gagctttctc gttcttgaat
     1501 cggaagatca tcctgaaaag gtaatagtgc gagaaatata aaatcaccag caatgccgag
     1561 aaaatgaggt ttcttgggat aaatggtttt agggtgcaac ttgtttcgag gaaaatttca
     1621 ttaaaataag gcaaggctag catcacggct ataattccaa aaaaacctag gacgttcctt
     1681 tttatgaatc ccacattttt attatagcac atattatcaa actggataaa catttgatgg
     1741 ggtaatcctt ctaagtcagg tcaaattgaa aatatcattt tagtttttgt ctccaacaca
     1801 ttcataaagc aattctccga aaactacata aaatagcatg aaaaaaaaca tgaataccac
     1861 aaatgttgtt aatgaatatt caacatcaaa atacgaatat atgtggtatt ctcgatgata
     1921 caatggggaa atcacaaatg agtagacaaa tatggaaatg aaaccagcga ttgtttttgc
     1981 aaaccatctt ttgcgataga atggaatacg atcagcccct ttttcacccc gaaaaatacc
     2041 ttgtacagat aaaaatgtga aaaatacatc acgataagag agattgacaa gaaaaaatag
     2101 ttttcgctat gcattgaacc tgatgaatcg agagcaacca tattgaggaa aggtgttgtc
     2161 actataacca tcaaaacccc ataagtgcct aaaatcaaat ctcctatgcg ccccatactt
     2221 ttattgtaat gtattgcttt gattttggga agaagagaaa atacctctgt tgaaataagc
     2281 attacccgca ggcatttgaa acagtgacgt tcataatggc cttgccgtcc tgcccgatgg
     2341 ctctgtacat cttttccagc gcctcaaggc catcctccag ctcgcaagag atcgttgttg
     2401 acatcgggtt tgtgaatgtc tcgagcccca tgccggagat tgcctggatt gcctccatga
     2461 ccctctcgcc gattttttct gtccccagcg ggtaaactga aacctgtatc atgactttca
     2521 tttttgcacc gtttttctga aactggccac aatcgctgcc cccttcttgc tccccttgat
     2581 gttcccccag gcctcttttg ccccaaaaca gcaattatct gcggggagga aatggacgca
     2641 ggtagaaatg ctggccaatc ctggcaaaaa gtcttgtagc tttgagaatg tcaggtattt
     2701 tgcggttttg tagacctcga ccccttccct ggtttttgcc catgggctgt ccgggttaag
     2761 aaccacgatt gcggcaatgc cattcggtct caggatcctc caggtttctt ttgccgcttg
     2821 ctcctggtca tccacaaact cgagcgcaaa cgagcagagg accctgtcaa aggccatgtt
     2881 ttcaaaaggg agttcggtca gtttgccatt taccaacctt gtgcttgcta gctcgggttt
     2941 ttttgaggcc gcatcaagca tctttgttga ttcatcgatg cctgtgacct tcattcctga
     3001 ttttgccaac aaacttgtga aaatgccagt cccgcaacct gcatcgaggc actcaaggtc
     3061 tttttgtggc ctgatggcct tcattattgc tgatgcctcg gccaaaagta ttgctttccc
     3121 aagttttgtt gcaaaaaatg aatcataatc tgaagcaaag ctgtcgaata gtgccatgtc
     3181 tgcaattata cctgcatttg tttgctgttg gttataagtt tgggtattcg atgatgattg
     3241 tttttgtttc cccctgccat gtgacctttg cgccaagcgc ctcggcgaca aatcttagtg
     3301 gaacaagtgt tttgcctgac acaatgaccg gtggggaggc gagtttgtat tcagcgccgt
     3361 tgactttcgc ctctttcttc ccgatccata tctcgacgat ggttttgcca agcttgtaga
     3421 tgaccttctt ctcggatgcg acccatgtga tctcggcgga aagtctttcg ccgatgaacc
     3481 tcatggggac aaacgtcgag ccggcgagga tcattggtgg aacatcaagt gtgtacgttg
     3541 ctccatcatc ggaggtggca gtttttgagc cgatctgcat tgtcaccata acatattttg
     3601 tgaccgtcac tctggcagtt gcagaaagcc cgttttcatc cgttgaagtg acaattattt
     3661 ctgtcagggt ctctgtttct tttattgttg cggtgaatgc gccgtcatct ttctgagaaa
     3721 tatccgcaac aagctccttc ccttggggtg ttttgaagga cagcttggcg tctggatcgg
     3781 cctttccgct taaaacaatc tcacccttct tctggacaac gaccccaggg tttatggtta
     3841 cctttggtgg tgtcactccg gttgcaaagt ttgctgcctg tatttcagaa agtgccccac
     3901 ctttgcaggc aaagattatt ttgagctcat gcttgccttt ttggaggcca gagaggatga
     3961 gttctcttcc ctgtatccag tccgaacctt ttgcgccatc aatgctgtat gaatagtcaa
     4021 gattctgatt gaaagatgct ttccagtcag aattctggga gctttttggt gcctgcagtt
     4081 ctggcagtgg acactcgttt gatgcagtct ggaggccgaa gcaccatttc tcgtaacaga
     4141 atgctgatga atgttcgatc tcgaatgcgc acttcccacc ccttgtgtcc agctctatgt
     4201 tgatattgtt ctggccgggt tttatggttt gggtcatctc cttgaatgtg aagctgtctc
     4261 tatcaaatcc tttccacgcg gccttgtatt ccctcttcga gttgatggtg aaagtttgtt
     4321 tgaagctatc cagttcgact gttttttgca tccagggtgg atcaagtatg ttttctccaa
     4381 gtgtggccat tggtatgcca ggtttttcga gattgaacga cagcatacac cccttgtctc
     4441 ttcttgtgaa gataccagcg taggcaagat tttctggcaa attccctagt ccattgtcca
     4501 gtatataggt ttttgcgggt tctgcctcgt cgtagaaaac ctgtatgcca tcaaactctc
     4561 ccatgatctg ttttcctcgc cagacaatcg aatcgttctg tattttcacc attctgttgc
     4621 cgagctttgc gcttccaagc actttcagct gtggtgttgg cccgactagg ataacgggtt
     4681 ttttagtgct tcctgcgaaa ttggatgtaa cgctttcccc accatatttt tgcacccatg
     4741 cttcggccct tttcattaca tcttcgccat caccaggctc atagcataca agaggttttt
     4801 gccacttaag caaggatatg atgttcgcag tagacatgct gtgtacgcga tcgtcatcca
     4861 ttggtatgat cccgttgtaa tccgtaaaaa cgatccctgt tgccattttg ggtagctgat
     4921 acagttttgt tttgcctgac agaacaaagc tctggctttc tccatcgaca tagtatacca
     4981 tgaagcgtgt tggacactct gttgaatcgt tgtttttaag gctaagagta tcaatgattt
     5041 ttccatcctt gttttccctc aggaggccga atgatgtgga caccaatttt ggtgaattga
     5101 aaaatactga ccagaaatca gtcagatcaa ctcctgacac ctcttctagc gtagctttca
     5161 agtctttcaa
//
LOCUS       DAOSRI010000118         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14814_length_5126_cov_3.382370, whole genome shotgun sequence.
ACCESSION   DAOSRI010000118 DAOSRI010000000
VERSION     DAOSRI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14814_length_5126_cov_3.382370"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3800
                     /locus_tag="PK717_04505"
     CDS             <1..3800
                     /locus_tag="PK717_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR46954.1"
                     /translation="GRKTGQTIASDSDGHGTSVCSVVCGETLGVAPKAKLIVGSVIPD
                     GSGNISQILGGLQWITDPDDDPSTDDYPRVVNMSFGASGTMDYLKTAIKNLRKFGILP
                     IASIGNDGEGSSSNPGNMPEVLSVGSVDYGLKASGFSGGAEVVWENEDDSLTLVKPDL
                     CAPGENIRVATPRRTYDVADGTSFSSPHVAGICALLLEKRPDISPEDLTYLLSKTVVD
                     LGRPGRDRRYGEGLVDARDSLSLALSMEPKTVSVTWPTGSALWGDINVTSGDRSYVVS
                     REQAKSFTFLSRSGEETKIGAFGFESSTIAGSSVELKPLPEHSLDLTVHEEGTQNNLD
                     SKVTLKDSPLPTFEAPDGTLKLALPEGEHEFVATSFGHASRNFKINLSSDLSASMPLE
                     KAKIAFIDTRKPVWGISPEPIRGKLKPALDSTGLPYFIWSTLQGNVTAKQLGAFQYVV
                     WNSGGSLSAKDVGILGGYMDMGGKLVLTSDFYGASFIGQTDSTVFLDSYFACRPSDEG
                     GAALTHWDGRTYKSLALGNPSAYFSTSNLVPMSEKAKILLSYAGTSPKKYAGLKVSTL
                     KNQGVILGFTFPNVASEEDRGWLLKLCIESFEDTLPWKADIKTGDGKLPSGNIAIGGE
                     TIGFTSGSIFVPHLPLVATKIEISSFGYKKVTLDDRPEKLPSQIMLESAPLSGVSFDS
                     SSDGYLIFNDKNVEPIKFPAKGEVRLPEGKYKVVAVSRGREPVEIDITAPGRIALNMR
                     QSQKAAVVFEEADDIKLTLSEIGIPYKTIQQPTAADIVSSGLVVWNHPEKISSSQTEM
                     IGNVKTALLCGAGAIIAGPELCRAFGGPIQIDSATSPVYASIGENELQGILISNSKSM
                     RNYSISIPVISGGDVLARFIGQGASIIKQNNLIACSFGFQDVDLANVKAELIRRLAMV
                     LGFGEGRLLPGQIVSPSKAVNTKSVEISGFAPPASKTSITIDGVEKIVDLDPEGFFSF
                     WASLAEGKHECVLISQSNGLLARSAPHTVIIDTTPPSIAIYSPKGGKTPSLEIEVLAN
                     VRSASKVLIDGKPARVEGTLLRATVPSGSGSILISCEDEAGNRSEARANYTPSPGLFA
                     DAQKSRSFFEISQLACANILKNESDQFYPSESLSRGKMAVWVALLLGLDPKPGKSTYE
                     DVPKNSPEEPFIGALVQHGTLSGKGKFYPGSACTRELLVQVLSNGLRLGKRADKMPFS
                     DIKQDNVYSQAILKCIFAGIIDPAEKRLFSSGKFGLGQKLSREQACIMFYNALVYRAR
                     GEQ"
     gene            3797..4210
                     /locus_tag="PK717_04510"
     CDS             3797..4210
                     /locus_tag="PK717_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOR46955.1"
                     /translation="MILEQLKQAGYRITKPRKAVIALLEENDDMDAKQIYRTLKERGQ
                     DIDIATVYRVLDLLFRLGVVHRSNFTQQHAHFGFVKAVHMICKRCGKIDEKEIDNKIL
                     LDKMKDVLGDFSAQDVSVEVYGYCKTCSRSADGNV"
     gene            complement(4190..4393)
                     /locus_tag="PK717_04515"
     CDS             complement(4190..4393)
                     /locus_tag="PK717_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOR46956.1"
                     /translation="MPEGTVRWFNAKKGYGFITRADNGEDVFVHFSAIRADGFKSLKE
                     GDRVRFEIQETPKGVQASNVAIC"
     gene            complement(4529..>5126)
                     /locus_tag="PK717_04520"
     CDS             complement(4529..>5126)
                     /locus_tag="PK717_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR46957.1"
                     /translation="HFIQEGTGPRNPYGVMYENTEELGDFDAFENFDADGDGYTNIQE
                     INAKTYPGDFMDHPSPAKYKKCVTFFLSLDTTTKKPITVCVVDGKKVDLTPHGGACYI
                     KNGKSMAPLRIGIESLGGTVGWVASEKRIDIKKGSKLVGQMWVGKKNAKINGKDYPLD
                     APPEIVSGKTFVPVKAVGDALGAKLIWINRGKIANFVL"
BASE COUNT         1341 a         1187 c         1242 g         1356 t
ORIGIN      
        1 atggcaggaa gactggccaa actattgcaa gcgacagtga tggccatgga acaagcgtct
       61 gctcggtcgt ttgtggcgaa acacttggtg ttgctccaaa ggcaaaattg attgtaggtt
      121 ctgtcattcc tgatggctcg ggaaacattt cccagatttt gggtgggttg cagtggataa
      181 ctgatcctga tgatgatcct tcaaccgatg attacccgag ggttgtcaac atgtcgtttg
      241 gcgcttccgg gacgatggac tacctgaaaa ctgcaataaa aaacctcaga aaatttggaa
      301 tattgcccat tgcctcgata ggcaatgacg gcgaaggttc atcgagcaat cctggcaaca
      361 tgcctgaggt cctctccgtt ggctccgtgg actatggact caaagcgtct ggtttctccg
      421 gtggcgccga ggtggtgtgg gagaatgaag atgattcact gacacttgtc aaacctgatt
      481 tatgtgcacc tggagaaaac atcagggtgg ccaccccaag aaggacctat gatgttgctg
      541 atggtaccag tttttcttcc ccgcatgtcg ccggcatctg cgcgcttttg cttgaaaaga
      601 ggcccgatat atctcctgag gacctgacct atctcctctc aaagactgtt gtcgaccttg
      661 gtaggccagg cagggacagg cgctatggcg agggccttgt cgatgcaagg gacagccttt
      721 cacttgccct gtcaatggag ccaaaaactg tttctgtgac gtggcccacc ggttctgccc
      781 tctggggaga cataaatgtc acctctggtg accgttcgta tgtggtttca agggaacagg
      841 cgaaatcgtt tacttttctc tccagatcag gcgaggagac aaagataggg gcttttggtt
      901 ttgaatcctc aacgattgca ggcagttccg ttgaattgaa gccgcttccg gagcattcgc
      961 ttgacctaac ggtgcatgag gagggtaccc agaacaatct tgacagcaaa gtcacactga
     1021 aagattcgcc gttaccgaca tttgaggctc ctgatggaac actaaagctt gctttgccgg
     1081 aaggcgagca tgaatttgtc gcgacttctt ttggtcacgc atcgagaaat ttcaaaataa
     1141 acctttcgtc tgatttgtct gcatccatgc ccctcgaaaa ggcaaagata gcttttatcg
     1201 acacaagaaa gccagtctgg gggataagcc cggaaccgat taggggtaaa ctcaaaccgg
     1261 cccttgattc aacaggactg ccctatttta tatggtcaac ccttcaaggt aatgtgacag
     1321 ccaagcaact tggtgctttc cagtatgtgg tctggaattc tggaggttca ctctcggcca
     1381 aggatgttgg tattcttggc ggatacatgg acatgggggg caagcttgtt ctgacctccg
     1441 atttttatgg cgcatccttc atcggccaga ccgattccac ggtttttctg gactcatatt
     1501 ttgcctgcag accaagcgat gagggtggag cggcattgac ccactgggac ggaagaacct
     1561 acaagagtct tgcacttggc aacccaagcg cctatttttc cacttcaaac cttgtaccaa
     1621 tgtccgaaaa ggcgaaaatc ctgctttctt atgctggcac aagccccaaa aagtatgcag
     1681 ggttgaaagt ttcaaccctc aaaaaccagg gcgtaatcct tgggtttacg ttcccaaatg
     1741 tggcctcgga agaagacagg ggttggcttt taaagctttg catcgagagt tttgaagaca
     1801 cattgccatg gaaggccgac ataaaaactg gtgatggcaa actgccttcc ggaaacatag
     1861 caattggcgg ggaaaccata ggttttacca gtggcagtat ttttgtgccg caccttcctc
     1921 ttgtggcaac aaagattgaa atctcgagtt ttggctataa aaaggtcacc ctggatgaca
     1981 ggcctgaaaa acttccttca caaataatgc ttgaatctgc acctctgtct ggagtttctt
     2041 ttgactccag ctctgatggg tacctcattt tcaatgacaa gaatgtggag cccattaagt
     2101 tcccagcaaa gggtgaagtc agattgcccg aaggtaaata taaggttgtg gctgtatcga
     2161 ggggaaggga gcctgtggaa attgatatca ctgccccagg caggattgct cttaatatga
     2221 ggcagtcaca gaaggcagca gtggtttttg aggaagctga tgacataaag cttacgctga
     2281 gtgaaattgg aataccctac aagaccatcc agcagccaac cgccgcagac attgtgtcct
     2341 ccggacttgt tgtctggaac catccagaga aaatctcaag ctctcagacg gagatgatag
     2401 gcaatgtgaa aacggccctt ctttgtggtg caggcgccat tattgctggc ccggagcttt
     2461 gcagggcttt tggtggacca atccagatag attcagccac ttcacctgtc tatgcgtcca
     2521 taggtgagaa tgagctccag gggatactca tatccaactc aaaaagcatg agaaattaca
     2581 gcatatcaat tcctgtaatt tcgggaggag atgtcctggc aagatttatt ggccagggtg
     2641 cctcaatcat aaagcagaac aatcttatag cctgctcgtt tggttttcag gacgttgatc
     2701 ttgcaaatgt caaagctgaa ttgatcagaa gattggccat ggtgcttggt tttggtgaag
     2761 gcaggctcct tcccggccag atagtttcac cgtcaaaggc tgtaaacaca aaatcggtag
     2821 aaataagcgg ttttgcaccg cctgcgtcaa agacttcaat aacaatagat ggcgtcgaaa
     2881 aaatcgttga tcttgatccg gagggttttt tctcattctg ggccagcctt gcagaaggaa
     2941 agcacgaatg tgtactaatc tcgcaatcaa atggccttct ggcgagatca gcgccacata
     3001 ctgtaataat agacaccaca ccgccaagca ttgccatcta ttcaccaaaa ggcggcaaaa
     3061 caccgtcgct tgagatagaa gttctggcca atgtcaggag tgcttcaaaa gtcttgatcg
     3121 atgggaaacc ggcaagggtc gagggaacgc tccttcgcgc aacagttccg tctggatcag
     3181 gctccatact catctcttgc gaagacgaag cgggcaacag gtctgaggct agggcaaact
     3241 atacaccgtc acctgggctt tttgctgacg cgcaaaaatc caggtcattt ttcgagatta
     3301 gtcagcttgc ctgtgcaaac atcttgaaga acgagagcga ccaattttac ccttcagaat
     3361 ctctttccag ggggaaaatg gctgtctggg ttgcactact gcttgggctt gacccaaagc
     3421 cgggaaaatc cacatacgaa gacgtgccaa agaattctcc ggaagagccg tttattggcg
     3481 cccttgtcca gcacgggacg ttatcgggta agggaaaatt ctatcccggt tctgcctgta
     3541 cgagagagct ccttgtgcaa gtcctctcca atggtttgag gcttggcaaa agggctgaca
     3601 aaatgccatt ttccgacata aaacaggaca atgtctattc tcaagccatc ctaaaatgta
     3661 tttttgccgg tataattgat cctgcagaaa aaaggctgtt ctctagtggc aaattcggcc
     3721 tcgggcaaaa gctctcaaga gagcaagcct gcataatgtt ttataatgct cttgtttata
     3781 gggcgagagg ggaacaatga tattggagca attaaaacag gctggctaca gaataacaaa
     3841 acccaggaaa gcggtcatcg cacttctcga agaaaacgat gacatggatg caaagcagat
     3901 atacaggacc ctaaaagaac gtggacagga cattgatatt gccactgttt acagagtgtt
     3961 ggaccttctt ttcaggcttg gtgttgttca caggagcaat ttcacccagc agcatgctca
     4021 cttcggtttt gtaaaggctg tgcatatgat atgcaagcgt tgtgggaaaa ttgacgagaa
     4081 agaaatagat aacaaaattc tgcttgataa aatgaaagac gtcctggggg atttctccgc
     4141 ccaggacgtc tctgtagagg tctatggtta ttgtaagacc tgcagccgat cagcagatgg
     4201 caacgtttga cgcctgcacg cccttcggtg tctcttggat ctcgaatcta actctgtcgc
     4261 cttctttaag ggacttgaat ccgtcagctc taatggctga aaaatgtacg aaaacgtcct
     4321 ctccattgtc agcacgggtg atgaatccat aacccttctt tgcgttaaac cacctaacag
     4381 ttccttctgg cattctactt ctctttcctt ctgctccttt ttcaggagct aatactaatt
     4441 tgggcttttt gcccttttat agtgaaaaag gcctcacaaa tccgaggccc aaatcacttg
     4501 gacttcaaat tcttattttg attatacact ataaaacaaa atttgcaatc tttcctctgt
     4561 ttatccagat gagttttgca ccgagtgcgt caccaacagc ttttacaggt acaaaagttt
     4621 ttccgctaac tatctctggc ggagcatcaa gcggataatc tttaccattg atcttcgcgt
     4681 tctttttgcc gacccacatc tgcccgacaa gtttgctgcc ttttttgatg tcgatcctct
     4741 tttcagaggc gacccagcca actgttccgc cgaggctttc aatacctata cggagggggg
     4801 ccatcgattt gccgtttttg atataacatg cgccaccatg tggtgtaaga tctacctttt
     4861 ttccatcaac aacacataca gtgatcggct ttttggttgt cgtatcgagt gagaggaaga
     4921 aagtaacaca ctttttgtat tttgcagggc tgggatggtc catgaagtca ccaggatagg
     4981 tttttgcgtt gatttcctga atgtttgtgt acccatcacc gtctgcgtca aaattctcaa
     5041 aagcatcaaa atcaccaagc tcttcggtgt tttcatacat tacaccataa ggattccttg
     5101 gccctgtacc ttcctggatg aagtgg
//
LOCUS       DAOSRI010000119         5120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_14839_length_5120_cov_3.951826, whole genome shotgun sequence.
ACCESSION   DAOSRI010000119 DAOSRI010000000
VERSION     DAOSRI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14839_length_5120_cov_3.951826"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2401
                     /locus_tag="PK717_04525"
     CDS             <1..2401
                     /locus_tag="PK717_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46958.1"
                     /translation="VAGVCALLMSTDKSYSAIDLRMAIKATADDLGSKGWDTRYGIGA
                     INPKAALDYLKKTGKVQVDINLPDKKVANLVIGDNHYRVDKSFALHLPPGKTNIELSS
                     FGFKKLAQEVNVIAGKSSSISYEMSELPKTSFKATMYGADGNPANVGQMRITGTNFSA
                     DSDENGNFDAEIPTDEYDVEFWSIGYVPKTMKIDFSKDLPKKIQLEKADVLVVALQYT
                     TPSQYYSRRFDKYCFRAFDEMGIKYCPVNPYKFDLTSDQMKMFDRVYWFAGENGLYSE
                     DVEILEDYLKEGGKLLISGSNLLYFESYRRDNSFFTDNYGVILKNSPTPLTTMMGVKD
                     DDIGDGLVFSFSGGDGASNQLGADGFVVNKGNGKPFMYYLGPKETSPADLGYTGLKLQ
                     TQNFLSIFLAFGFEGINSKEARQMLLSRVEKWFTSFGGVDITFEDEKGNPVYAKAKID
                     GIGDVETNDEGHLILSYLPQGKISLKVVPFGFAEQSFTATVTPGKTTRISLGLSNPTS
                     VKISGKVVDSATGTPMKCDFKIIGFETKQYSTDDAGNFSLTLPKYKYSLKIFKKGYMN
                     YWMDILENAEDLKVKMEKPKRVVALFQQIAPSWDTRSFAGIAQIYERVARGSGTDFDN
                     IIVPPGNVVDYEDMQQFSKIIWYCGFNDEIGASWWLDNLARYVNDGGSIAIIGQNVPQ
                     AISSRPELAKTFGFELLESNTSIYAVKGVKGDPIGDDLIFSLYHPFLRMGLLSVAPSM
                     KPVGSGVSCFDYLGGASAGVRVGTDKQKTLVLPFGFENMYADTKESQDLFKRIVSYLG
                     K"
     gene            2464..2808
                     /locus_tag="PK717_04530"
     CDS             2464..2808
                     /locus_tag="PK717_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR46959.1"
                     /translation="MTLGVIIKTALVRKGKTQAWLASKLGLSSMSVYRICNDLVKPRQ
                     DVLLGMAENLDLPSEFLIKTCIQTQYKTSEGFDLSRTKKVLEQLALEDPTLSADKKEA
                     LFKAAMVLSEID"
     gene            complement(2805..4286)
                     /locus_tag="PK717_04535"
     CDS             complement(2805..4286)
                     /locus_tag="PK717_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOR46960.1"
                     /translation="MDIFTKPLQVGRLIAGLGPDELREMARKDERTTEFGSASYVTKI
                     RNRSAKFTEIVGIEPTKSQVETILKVREYLKDKEVIYTERTMCQTPGHQIPCRLYITK
                     PYARVPYMWQNLLFPPTKLDRPFLTTVDVPEWPERKVIVNALDGVTYVLGTDYFGEVK
                     KSFLRMTMYRGKQDGGLGLHAGSKMIKVKDVNGKLVEKGIILFGLSGTGKTTLTCHHH
                     FLEGEEGVAIRQDDVVIIYPDTKCVGTEKNFYIKTEGLEPVGQPVLYNAAVARTAVLE
                     NIHVEENGKVDFLNYDVGTNGRAVVQRSDIKFTDNSIDIPKTDMVVFITRRDDIVPPV
                     AKLSPEQAAAFFMLGESIETSAGDPTKAGQSKREVGTNPFIVGPLDDEGNYIMDLLKK
                     NPHMQCFLLNTGKIGNKEKIRISDSVGILKQIARGAVKWRKDDYWGYEVLSECEGVDI
                     NRLDPRNYFTPEELKAKNDSLRKERRAWLDQFLSLDPDIQKVI"
     gene            complement(4301..4696)
                     /locus_tag="PK717_04540"
     CDS             complement(4301..4696)
                     /locus_tag="PK717_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOR46961.1"
                     /translation="MAIKVIIAKPGLDGHDRGAKVIAQALRNAGMEVVYTGLHQTPEM
                     IIRAAIQEDVDVIGVSILSGAHMSIVPRIIEKMKENGIWGKPLFVGGIIPADDAEELK
                     KIGVSAVFGPGTPTSKVVEFINSVVKDKK"
     gene            complement(4687..>5120)
                     /locus_tag="PK717_04545"
     CDS             complement(4687..>5120)
                     /locus_tag="PK717_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46962.1"
                     /translation="ESGDSIQMMKAGLLEGADIFCINKTDRPGGDVMEADLTFGLGLS
                     SRDWAPPIVKTIAKESIGISELYTQIKKHMEHLIKNDERSKKCIERYFSEIDFWLGSF
                     VFDKALEPERKKYIQKSFGEDIPPWTVAKTLWKNKMEEPWQ"
BASE COUNT         1345 a         1164 c         1193 g         1418 t
ORIGIN      
        1 tgtggctggc gtttgtgcat tgctcatgtc aaccgacaag tcttattcgg caattgactt
       61 gagaatggca atcaaggcaa cagccgatga tctgggctca aaaggatggg acaccagata
      121 tggtattggc gcaatcaatc caaaggccgc actggattat ctcaagaaaa ccgggaaggt
      181 gcaggttgac atcaaccttc cggacaaaaa agtggcaaac cttgtaatcg gcgacaatca
      241 ttacagagtg gacaagagct tcgcactaca ccttccacct ggaaaaacaa acatagaact
      301 gagttctttt gggtttaaaa agctcgccca ggaagtcaat gttattgcag gaaaatcatc
      361 ttccataagc tatgaaatgt ctgagctgcc aaaaacttct ttcaaagcaa cgatgtatgg
      421 tgcagatgga aatcctgcaa acgtcgggca gatgaggatt actggaacta acttcagcgc
      481 tgatagcgat gaaaatggaa attttgacgc agagattcca acggacgaat atgacgttga
      541 attctggtct attggctatg tgccaaaaac catgaagatt gacttctcaa aagacctgcc
      601 aaagaaaata cagcttgaaa aggcagacgt gctcgttgtt gccttgcaat acaccacccc
      661 aagccagtat tacagcagaa ggtttgacaa atactgtttc agggcctttg atgagatggg
      721 gataaagtat tgcccggtca atccatacaa gtttgatctt acaagcgatc aaatgaagat
      781 gtttgacagg gtgtactggt ttgccggcga gaatggtctt tattccgaag acgtagaaat
      841 ccttgaggac taccttaagg aaggtgggaa acttcttatt tcgggctcca atcttctcta
      901 ctttgaaagc tacagaaggg acaactcgtt cttcacggac aactatggcg ttatcctgaa
      961 aaattcacca acaccactta caacaatgat gggtgtgaag gacgacgaca taggcgatgg
     1021 ccttgtcttc tcgtttagcg gtggtgatgg tgcgtccaac cagcttggcg cggatgggtt
     1081 tgttgtcaac aaggggaacg gcaagccatt catgtactat cttggtccaa aggaaacttc
     1141 ccctgcagat ctcggctata ctggcctaaa gctccaaacc cagaacttcc tctcgatctt
     1201 ccttgcgttt ggctttgagg gaatcaatag caaagaagcc aggcagatgc tgctctccag
     1261 ggttgaaaag tggttcacca gttttggtgg cgtcgatatt actttcgagg acgagaaggg
     1321 caatcctgtt tatgcaaaag caaagataga cggcattggt gatgttgaaa caaacgatga
     1381 aggtcacctg attctctcat atctaccaca gggcaaaatt tccttgaaag ttgtcccgtt
     1441 tggctttgcg gagcagagct tcacagccac tgtcacgcca ggaaaaacga ccaggataag
     1501 tcttggcctg tcaaacccaa cttcagtaaa gatctccgga aaggttgttg actcagcaac
     1561 cggaacaccg atgaaatgcg atttcaagat cataggtttt gaaacaaagc agtattcaac
     1621 ggacgatgcc ggaaacttct cattgaccct tccaaaatac aagtattcgc tcaagatctt
     1681 caaaaagggc tatatgaatt actggatgga tatccttgaa aatgccgaag atctcaaagt
     1741 caaaatggag aagccgaaga gggttgttgc gctgttccag cagatagctc caagctggga
     1801 cacgaggagt tttgcaggga ttgcccagat atacgagagg gttgcaagag gttctggcac
     1861 tgatttcgac aacattatag tacctcccgg caatgtagtg gactatgaag acatgcagca
     1921 gtttagcaag ataatctggt actgtggctt caacgacgaa ataggggcct catggtggct
     1981 ggacaacctt gcaagatacg tcaatgatgg tggttcaata gccatcatag gccagaatgt
     2041 gccgcaggca atcagctcaa ggcccgagct tgcaaaaaca ttcgggtttg agttgctcga
     2101 atccaatacc agcatctatg ctgtcaaagg tgtgaaggga gacccgattg gtgatgattt
     2161 gatattctcg ctctatcacc cattccttag aatgggactt ctttcggttg caccatcgat
     2221 gaagcctgtt ggttctggag tgtcctgttt tgattatctt ggcggagcat cggcaggagt
     2281 gagggttgga accgacaaac aaaaaacgct tgtgctccca ttcggttttg agaacatgta
     2341 tgcagacacc aaggaaagtc aggatctttt caagaggata gtgagctacc tggggaagta
     2401 aaatagtcta aaagcgacaa aaaaccgttt gacaatgtca tatcacacaa taaaatgtgt
     2461 gatatgacat tgggagtgat tataaagaca gcgttagtcc gcaaaggtaa aacacaggcc
     2521 tggcttgcat caaagctggg cctttcttct atgagtgttt accggatatg caatgatctg
     2581 gtcaagccga gacaggacgt tcttctgggg atggctgaaa accttgatct tccatctgag
     2641 tttctcataa aaacatgcat tcagacacag tataaaacat cagaggggtt tgacctttcc
     2701 agaacaaaaa aggtcctcga gcaacttgcg ctcgaggacc ccacgttaag cgctgacaag
     2761 aaagaggcgc tatttaaggc cgcaatggtt ttgtctgaga tagactagat cactttctgg
     2821 atatctggat caagggagag gaactggtcg agccaggccc tgcgctcttt tctgagagag
     2881 tcattctttg ctttcagctc ttcaggcgtg aaatagtttc ttgggtcaag cctgtttatg
     2941 tcaacacctt cacactctga gaggacttca tacccccaat aatcgtcctt gcgccacttt
     3001 acagcgcccc ttgcgatctg tttgagaatt ccaaccgagt ctgatattct gatcttctct
     3061 ttgttgccaa tcttgccagt gttgaggagg aagcattgca tgtgtggatt ttttttgaga
     3121 aggtccatga tatagttgcc ttcatcatcg agtggtccga caataaatgg gtttgttccc
     3181 acttcgcgct ttgactggcc agcttttgtt gggtctccgg ctgatgtttc gattgactca
     3241 ccaagcatga agaaggcagc tgcctgctct ggagacagtt ttgccaccgg cgggacaatg
     3301 tcgtccctgc gagtgatgaa aaccaccatg tcggtttttg ggatatcgat gctgttatcg
     3361 gtgaatttta tgtcagagcg ctgcacgact gctcttccat ttgtccctac atcgtagttg
     3421 aggaaatcga ctttgccgtt ttcttcaaca tggatgttct ccaggacagc agttcttgcc
     3481 acggccgcgt tgtaaaggac gggctggcca accggctcaa gaccctctgt tttgatgtag
     3541 aagttctttt ccgttccaac acactttgtg tctggataaa taattacgac gtcatcctga
     3601 cgaattgcca caccctcttc gccctcaagg aaatggtgat ggcaggtgag ggttgtcttg
     3661 cctgttccag aaagaccaaa caggattatt cccttttcga cgagctttcc gttgacgtcc
     3721 ttgaccttga tcatctttga acctgcatga agaccgagtc cgccatcctg cttgccgcga
     3781 tacattgtca tcctgaggaa tgattttttg acctctccaa aatagtccgt ccccagaaca
     3841 taagtcacac catcgagcgc atttactatg acctttcttt ccggccattc cgggacatca
     3901 actgttgtga ggaaaggacg atcgagtttt gttggcggga agagcaggtt ctgccacata
     3961 taaggaactc tggcgtatgg ttttgtgatg tagagcctgc agggaatctg gtggcccggt
     4021 gtctggcaca tcgttcgctc ggtatagatg acttctttgt ctttcagata ctctcttacc
     4081 ttcaaaatag tctctacctg tgatttggtt ggctcaattc caactatttc tgtgaatttt
     4141 gccgacctat tcctgatttt tgtaacatag gaagcggaac caaattcagt ggttctctca
     4201 tctttcctgg ccatttccct cagctcgtcc ggacccagtc cggcaattag acgccccact
     4261 tgaaggggtt tagtaaaaat gtccatcgtt cctcctaaat ctattttttg tctttcacaa
     4321 cagaattgat gaattcaact acttttgagg ttggcgttcc agggccaaaa actgccgaaa
     4381 caccaatttt ctttagttcc tcagcatcgt cggctggtat gattccgccc acgaaaagcg
     4441 gtttccccca gatgccgttt tctttcatct tttcaatgat tcttggcaca atggacatgt
     4501 gcgcaccaga aaggattgat acacctatga catcaacatc ttcctgtatt gctgccctta
     4561 tgatcatttc aggtgtctgg tgaaggccgg tgtaaacaac ctccatccca gcattgcgaa
     4621 gagcttgggc gattaccttg gcgcccctgt cgtggccgtc gagaccaggt ttagcaatta
     4681 tgacctttat tgccatggtt cctccatttt atttttccac aaagttttgg caacagtcca
     4741 cggtgggata tcctcgccga aagacttttg gatatatttt tttctttccg gctcaagcgc
     4801 tttgtcaaaa acaaaacttc caagccagaa atctatttct gaaaaatacc tttctataca
     4861 ttttttgctt ctctcatcgt tttttatcaa atgctccatg tgttttttta tttgcgtata
     4921 gagttctgat ataccgatgc tttctttggc tatggtttta actattggag gtgcccaatc
     4981 tcttgatgac aagccaagtc caaatgtcag atcggcttcc atgacatcgc caccaggccg
     5041 atctgttttg ttgatgcaaa agatgtctgc gccctcaagg agcccagcct tcatcatctg
     5101 tatggaatcg cctgactctg
//
LOCUS       DAOSRI010000120         5050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15085_length_5050_cov_3.999600, whole genome shotgun sequence.
ACCESSION   DAOSRI010000120 DAOSRI010000000
VERSION     DAOSRI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15085_length_5050_cov_3.999600"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK717_04550"
     CDS             complement(<1..298)
                     /locus_tag="PK717_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOR46963.1"
                     /translation="MFTILEKRDLADKICEYVFEAPLVAKKARPGQFVILRLDEEGER
                     IPITLADTDPKTGRIKIVVQAIGTTTNRLHCFEKGDAITDIIGPLGTPTHLDGFK"
     gene            438..2111
                     /locus_tag="PK717_04555"
     CDS             438..2111
                     /locus_tag="PK717_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOR46964.1"
                     /translation="MKKFFILTLALLIVLPLLPDGFGTLAQEVSDLKLIVVAADFSDA
                     PHKSEFSNMCDELFSESGQSLNRFYKEVSSGTIKVSQGSFLKGGWVRMPKPITYYAQD
                     NEESFDIRCHEMIDTLMHEAAKSGLDFDEYIKSWSQPPCVCIVVSGGCEGYTNFPKND
                     GFWPHMSMSSVTIDGKTIEFLYVLVCEEQSVNSTASQVMAHEFGHIMGLADLYDYDCG
                     GPFKEGDCGYPVTCFDIMAARHKGLGMSGFHRERMGFLRPIVVGQSGEYDIPPITTNA
                     NGSYLVVPIEGTKEYLALEYRKKEGTDAFWGGIPSEGLLVYKIDDSVTYRHRFNSGDK
                     NNHYAVEILNPGKTEWHENACYSIESGHTVASPKTDPSTLPFDKNYTKIVTVEVLSKL
                     GPTLKVRITITPKENVFFSMDKNWAVTGDLHKTCLRVRMFNKGSNPVMLDCDPQVFGE
                     KSVKLNPDEGKDISLVLSYPKDKLGERLVEKSVSVESENQTFFFKVFLRNAYFIMDYN
                     SNGVIEEEDLLKIFSSLSTKEMKYDLDGDGAVGYGDFIVASRYIGVKYK"
     gene            2108..2968
                     /locus_tag="PK717_04560"
     CDS             2108..2968
                     /locus_tag="PK717_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR46965.1"
                     /translation="MKKIGTIFLALLITFLPSTFAQVGEKTIDSELVLVSPGMVVDWH
                     FEDKVLLDGLYRGMLEPDETTSEHVWAGQGCINDYCFSEEGGSPDPVIQGSSLRCQMR
                     FFVPSDFSGEDPVSARLRIMDPSGQDFGNGMLYMMLVKPVKAYFVAGEKPVLVREIEM
                     DGCTPGKTLSEKTLTAKTAPTIVSGNMMIPFRLLGEIIGAEVGWNNSIGEASYIMGPK
                     KVLLKKGIDKARLVYPNLEKTLAMSTAPVNIKGNIMVPLRFVSTALGGSVEWDQFTNT
                     AIVTFPGCSN"
     gene            3092..3511
                     /locus_tag="PK717_04565"
     CDS             3092..3511
                     /locus_tag="PK717_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOR46966.1"
                     /translation="MTRRDYGLSDNMRLTKQRRVILDILRNTDTHPTADWVYAKARRQ
                     IKNLSLGTVYRNLGILTREGLVQKLELGFGQARYDGRTDGHIHIVCDKCGVIKEMMQP
                     EKFAEINEIQDKSGFQINHVAMEIHGICPKCKTDGKE"
     gene            3517..>5050
                     /locus_tag="PK717_04570"
     CDS             3517..>5050
                     /locus_tag="PK717_04570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOR46967.1"
                     /translation="MQEPKISDNAKVVLEKRYLAKDMDGKVIETPLGMLQRVARAIAD
                     GDRAHGATPEQVEETYKSFLEMMVNLEFLPNSPTLMNAGRELGQLSACFVLPVEDSIE
                     SIFEAIKHTAMIHKSGGGTGFSFSRLRPKNDIVKSTNGVSSGPVSFLKVFDAATEAIK
                     QGGTRRGANMGILRIDHPDILEFIKCKSEDKTISNFNISVGITEDFMRAVDADEEYPL
                     LNPRGKAVVERLRAKEVFDLIVDMAWKNGEPGIIFLDRLNKDNPTPTLGEIESTNPCG
                     EQPLLPYEACNLGSLNLATVVIDGQIDWDKLAKLIKKSVHFLDNVIDVNRYPLESITK
                     MCRENRKIGLGVMGFADMLFLLGISYNSQEAVDLANKIMAFVQDKGREASRELAETRG
                     PFPNFHISTYAQGDEPPVRNATITTIAPTGTISMIAGVSSGIEPVFAISYVRNIMDGK
                     QFFETNTIFQQELEKRGLVSDELLAQISSEGTIAHIDQLPPDIKRVFVCAHDISPYWH
                     VRM"
BASE COUNT         1430 a         1057 c         1227 g         1336 t
ORIGIN      
        1 ttttgaatcc gtccagatgg gttggagtgc caagaggacc aataatgtcc gttatggcat
       61 cacccttttc aaaacagtgc agtctgtttg tggttgtccc gattgcctgg acgactattt
      121 tgattcgccc agtctttggg tccgtgtcgg caagagttat tggtattctc tcgccttctt
      181 cgtcgagcct cagtatcaca aattgtcccg gcctggcttt tttcgcgaca agcggagcct
      241 cgaagacgta ttcacaaatt ttgtctgcaa ggtccctttt ctcaagaatg gtaaacaacg
      301 cacaccccct ttggttttgt ttacaaccgg aagtaagtat agattttggc gggtttattg
      361 tccagtatga agcacccaaa aaatggcctg ccaattcgtg caagaatttt aaactacggg
      421 gcgttataat ccctttcatg aagaagtttt ttattttaac actggccttg ctgattgttt
      481 tgccattact gccagatggt tttggcactt tggctcagga agtttccgac ttaaagttaa
      541 ttgttgtcgc agctgacttt tctgatgctc cgcacaaaag tgaattttca aatatgtgcg
      601 atgagctttt ctcagagagt ggccagagcc ttaacaggtt ttataaagag gtatcatccg
      661 gcaccataaa ggtttcgcag gggtcttttc ttaagggtgg ctgggtaagg atgcctaagc
      721 caataacata ttacgcgcag gacaacgagg aatcttttga catcagatgc catgagatga
      781 ttgacacatt gatgcacgaa gctgcaaaaa gtgggcttga ttttgatgaa tatatcaaga
      841 gctggtcgca acccccgtgt gtttgtattg ttgtgtcagg tgggtgtgaa ggatatacca
      901 atttcccaaa aaatgacggt ttctggccgc acatgtccat gagttccgtg accatagatg
      961 ggaagacaat agaatttctt tacgtccttg tttgtgaaga gcaatcggtc aacagcactg
     1021 caagccaggt catggcgcat gaatttggtc acataatggg tttggcggac ctttatgact
     1081 acgattgcgg agggcctttc aaggaaggtg attgcggcta cccggtgacc tgttttgata
     1141 tcatggccgc caggcacaaa ggacttggga tgagcggttt ccacagagaa agaatgggtt
     1201 tcctccgccc cattgttgtt ggccaaagcg gtgaatacga cattccaccg ataaccacca
     1261 acgcaaatgg gagctacctt gtcgttccta ttgaaggaac caaggaatat cttgcgctgg
     1321 aatacaggaa gaaagaaggc acagacgctt tctggggtgg aataccgtcc gagggcctct
     1381 tggtttacaa aattgatgat tcagttactt acaggcatcg cttcaacagt ggcgacaaaa
     1441 acaaccatta tgcagtcgaa atcttgaatc ctggcaaaac agaatggcat gaaaatgctt
     1501 gctactcaat tgaatctggc catacggtcg cttctccaaa aactgatcct tcaacgctcc
     1561 cttttgacaa aaactataca aaaattgtaa cagttgaggt tctttcaaag ctcggtccca
     1621 cattgaaggt cagaataaca ataacaccaa aagaaaacgt ctttttctct atggacaaga
     1681 actgggcggt gactggggat ttgcataaga catgccttag ggttagaatg ttcaataaag
     1741 gaagcaatcc agtgatgctc gattgtgatc cccaggtttt cggggagaaa agtgtcaagc
     1801 taaatcctga tgagggcaaa gacatttcgc tggttctttc ttatccaaaa gacaaattgg
     1861 gggagagact tgttgaaaaa agtgtctctg tggaatctga gaaccaaacc tttttcttta
     1921 aggtatttct gaggaatgct tattttatca tggattacaa ctccaatggc gtaattgagg
     1981 aggaagatct gttgaagatt ttttctagcc ttagcactaa agagatgaaa tatgatctgg
     2041 atggagatgg cgccgttggt tatggggatt ttatagtggc ctccagatat attggggtta
     2101 agtataaatg aagaagattg gaacaatctt cttggcactt ttgataactt ttctccccag
     2161 tacgtttgcg caggtgggtg agaaaacaat agattctgag ctggttctcg tctccccagg
     2221 aatggttgtt gattggcatt ttgaggacaa agtactgctt gacggtcttt acaggggaat
     2281 gctggaacca gatgaaacga cttcagagca tgtctgggca gggcaggggt gcattaacga
     2341 ctattgcttc tctgaagagg gcggatcgcc agaccctgtc atccagggtt catccctgcg
     2401 ttgtcagatg aggttttttg ttccaagcga tttttctggt gaagatccgg tctcggcaag
     2461 gcttaggata atggatccat ctggccagga ttttggcaat ggaatgcttt acatgatgct
     2521 tgtaaaaccc gtcaaagcat atttcgttgc tggtgaaaag cctgttcttg taagagaaat
     2581 tgagatggat ggctgtactc ctggcaaaac actttcagaa aagacattaa cagccaaaac
     2641 agcaccaaca attgtttctg gaaacatgat gattcccttc agactccttg gcgaaatcat
     2701 tggtgcggag gttggctgga ataattcaat tggagaggct tcatatatca tggggcccaa
     2761 aaaagtgctt ttaaaaaagg gaattgacaa ggcaagactc gtttacccaa atcttgagaa
     2821 gacccttgca atgagcaccg ctccagttaa tataaaaggg aacataatgg ttcctctcag
     2881 atttgtttca acggcacttg gcggaagtgt tgaatgggac cagttcacaa acaccgcaat
     2941 agttaccttc ccgggctgtt ccaactgata ctacaattat aattggcagg gagtcatctt
     3001 gcaccggatt ccctattgtg taataatttg gtgttatttt tttaacaata aaaaagccta
     3061 gcgtgcgcca ggccatcttt cagggggaag aatgaccaga agagattatg gtttgtccga
     3121 caacatgaga cttacgaaac aaagaagggt gattctggat atattaagaa acactgatac
     3181 tcatccaact gctgattggg tttatgcgaa agctcgaaga caaatcaaaa acctgagcct
     3241 cggtaccgtt tacaggaacc tgggcattct taccagagaa ggtcttgtcc aaaaacttga
     3301 acttgggttt ggccaggcca gatatgatgg aaggactgat ggccatatcc atatagtgtg
     3361 tgataagtgt ggtgtaataa aggaaatgat gcaacccgag aagtttgccg aaataaatga
     3421 aatacaggac aaatcaggtt tccagatcaa ccatgttgcc atggagatac atggcatttg
     3481 cccgaagtgt aagactgacg gaaaggagtg atccacatgc aggaaccaaa aatctccgat
     3541 aacgccaagg ttgtccttga aaaacgctat ctggcaaaag atatggatgg caaagttatc
     3601 gaaacaccac ttggcatgct ccagagggtg gccagggcca tagccgatgg agatagggcc
     3661 catggtgcaa ctcctgaaca ggttgaggaa acatataaat cattccttga gatgatggtg
     3721 aatcttgagt ttttgcccaa ctcgccaaca ctcatgaatg ctggcagaga attgggccag
     3781 ctttccgcgt gctttgtttt gccggttgag gattcgatag aatcaatctt cgaggctatc
     3841 aagcatactg ccatgatcca caaatcaggt ggtggtactg gattttcctt ctcaaggctc
     3901 aggccgaaaa atgacatcgt gaaatcaaca aacggtgtct catcgggacc agtatctttt
     3961 ctgaaggtct ttgatgctgc caccgaggcg ataaaacagg gtggaacacg caggggcgcc
     4021 aacatgggca tcctgaggat tgaccatcct gacatcctcg agttcatcaa atgtaagtcc
     4081 gaagataaaa caataagcaa tttcaacata tcggttggca tcaccgagga tttcatgagg
     4141 gcggtggatg ctgacgaaga atacccatta ttaaacccaa ggggcaaagc tgttgttgag
     4201 aggcttaggg caaaagaggt ttttgacctc atagttgata tggcatggaa aaatggtgag
     4261 ccaggaataa tcttccttga caggctcaac aaggacaacc caacgccaac attgggtgaa
     4321 atagagtcca cgaacccctg cggtgaacaa cctttgcttc cttatgaggc atgcaatctt
     4381 ggttccctca atcttgccac tgtcgtgatt gatgggcaaa tcgactggga caagctggca
     4441 aaactcatca agaagtctgt gcacttcctc gacaatgtta tcgatgtaaa caggtatccg
     4501 cttgagtcga taacaaaaat gtgccgtgaa aaccgcaaga ttggccttgg ggtgatgggg
     4561 tttgctgaca tgctcttcct gcttggcata tcatataact cgcaggaagc ggttgatctg
     4621 gcaaacaaga tcatggcatt tgtgcaagac aaaggcaggg aagcttcaag agagcttgcc
     4681 gaaaccagag gccctttccc caatttccat atctcaacat acgcgcaagg cgacgaacca
     4741 ccagttagaa atgccacaat aactaccatt gccccaactg ggaccataag catgatagcc
     4801 ggggtttctt caggaataga gccggttttt gccatctcct atgtccgcaa catcatggat
     4861 ggcaagcaat tctttgaaac caatacaatt ttccagcaag agcttgaaaa gaggggcctt
     4921 gtctctgatg aactgcttgc acaaatatct tccgagggca ccatcgccca cattgaccaa
     4981 ctcccaccgg acatcaagag agtatttgtt tgtgcccatg acatctcgcc gtactggcac
     5041 gtcaggatgc
//
LOCUS       DAOSRI010000121         5044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15110_length_5044_cov_3.996392, whole genome shotgun sequence.
ACCESSION   DAOSRI010000121 DAOSRI010000000
VERSION     DAOSRI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15110_length_5044_cov_3.996392"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="PK717_04575"
     CDS             complement(<1..1707)
                     /locus_tag="PK717_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOR46968.1"
                     /translation="MSVILAGALLFGLLPIGSAQAASVTVSGVISSVSCYSSIQVSSP
                     SLGKVFDVAIVGSTIITVNGAVSPCTDLQPNMSVTVVGVDNGGRIIASKIAAQGAGSV
                     KKITATGKIVSTYCPNAIVVATSSGNIQFDISRAMIMGPTGSLSCEDLEVGDPVIVSG
                     EQRADGSYYASYVKVNTGSVGFVATGKISSIDCSSGEMVLQATSQTVKILIGSARFTL
                     NGKSVSCDELKVGDSAQVKGTADKGVYTAQTVAVTRETGTFNVTGKIASISCPSVISI
                     TVSGGMLTIKITQNTIIKKGGVVASCGDLQVGDSATVSGTVENGVYVADIINATSQVQ
                     TFTLSGTIEATNCPSEITLKTSSGTFTIDVSQAQIKIGGVVKQCSDLKQGDIAYVSGV
                     MQNGKKIAQTVTVTSSGQVTVSIEGTVFSKNCQTKMVTVKKQNGIIVQVVLPQSFTRN
                     GNAATCDDIKIGDNAYIEGTQSGATIVATKAEFAGETGGGETKTVSGKIITIDCQALL
                     MVLETESGTIQVRFTPLTKFFRNNISAECSELVPGDTASVTGIESQGILVAETVYAIG
                     EGG"
     gene            complement(1831..2346)
                     /locus_tag="PK717_04580"
     CDS             complement(1831..2346)
                     /locus_tag="PK717_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46969.1"
                     /translation="MRARATSLLASSLLLVALLSGCGGSTVKTEKLEVKKSYEIIPKI
                     AAHYPVYDGAFENEIYGKRIDVKTVAEKVFGVEVSGPLFAGGDPAEIFEWYMQKAKDD
                     GWGKFEGGTSYVPYDTEKEYGFLAISKGRRTLSLEFRRKPGWEYTRIQYLGMAYHKDG
                     AWLDDGLEYQK"
     gene            complement(2312..2872)
                     /locus_tag="PK717_04585"
     CDS             complement(2312..2872)
                     /locus_tag="PK717_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46970.1"
                     /translation="MKNIRNFALLLGLLATLASCAQASRELDVDKLANPLKAGDKPDI
                     EIVEPVVTEAPFKADKTRLNELSPYVPIYKDAKFDSTRSFIVGEDNENIGKTKVTAET
                     YHNATAEEVYTWIVDHLTDTGLRVAKKEEINDQDPLIFVEADGTAHITAIGKESFMKI
                     DVWTLTAYPGWVRVNYEGTSDKPFGK"
     gene            complement(2869..3336)
                     /locus_tag="PK717_04590"
     CDS             complement(2869..3336)
                     /locus_tag="PK717_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46971.1"
                     /translation="MKYFAVIAMVVVMLSGCTAGSISMVELPKPEDKTGLPSPLPEYV
                     PVFRPSSDNPANPLRVLNEKVGQTDLVREITGTLYTSSNPLRIYEWYKDEFAAKGWAT
                     NYDLPYSEKFQTGKLTCIKGDLVLTVTINYDPVQSRTEILYLGNKFTKPQDGK"
     gene            3443..>5044
                     /locus_tag="PK717_04595"
     CDS             3443..>5044
                     /locus_tag="PK717_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOR46972.1"
                     /translation="MTDNYTAQDIKVLDGIEHVRLRPAMYIGSTDERGLHHLVYEVVD
                     NAIDEAMGGWCKNIEVTIHPDGSISVSDDGRGIPVDLHPQLGIPGVTVALTRLNAGGK
                     FDKKSYKVSGGLHGVGVSVVNALSTWLMVTIKKDGKVHRQRFRKGAPETDLEITGETK
                     ETGTIVTFKPDSEIFSTVEFDFKTLSHRLRELAYLNSGLRIAIKDEVTGKFEEYKFDG
                     GLTEFVEYLSEGKEAIHREIIHFNDTKDDMYIEFAMQYTTGYGETIFSFVNSIATADG
                     GAHVTGYHAALTRVLNDKAKLLGIIKTEEDSLSGEDTRDGLVCVLSIRIADPQFEGQT
                     KGRLGNMTAKSGVERYTQEKLSYYFDKDTQTIKNILEKCILAKKAREAAKKARDLVRR
                     KSLLESSVLPGKLADCSERDPSKSELFIVEGESAGGSAKQGRDRKFQAILPLKGKILN
                     VEKANLNRVLSNAEVRALITSLGCGIGEDFDIARLRYHKVVIMADADVDGAHIATLLL
                     TLFYTHFRQLIENGHIYIAQPPLYKI"
BASE COUNT         1339 a         1270 c         1147 g         1288 t
ORIGIN      
        1 tccaccctcg ccaatagcat agacagtctc tgcaacaaga atgccctgcg attcgatgcc
       61 agtaacactt gctgtgtcac ccggcaccaa ttcggaacac tctgcagata tgttgtttct
      121 gaagaatttt gttagcggtg taaatctgac ctgtatagta ccagattcag tctcaaggac
      181 catcagaagt gcctggcaat caatggtgat tatcttgcca ctgacagtct tggtttctcc
      241 accaccagtt tctcctgcaa actcagcctt ggttgcaact atcgttgcac ccgactgagt
      301 tccttctata taagcattgt caccaatctt tatgtcatcg caggtggccg cattgccatt
      361 tcttgtaaat gactgcggaa ggacaacctg tacaataatt ccgttctgtt tttttaccgt
      421 gaccatcttt gtctggcagt ttttcgagaa caccgttccc tcgattgaaa cggtcacctg
      481 gccagagctg gtcactgtta ctgtctgggc aatctttttc ccgttttgca tcacgccaga
      541 aacatatgca atatcaccct gtttgaggtc agaacattgc ttcacgactc caccaatctt
      601 tatctgggcc tgcgatacat caatcgtaaa cgtgccagaa gaagttttca gcgtaatctc
      661 agaagggcaa tttgttgctt caatcgtgcc agacagtgtg aaagtttgga cctgcgaagt
      721 ggcattgatt atgtctgcaa catagacgcc attttcaaca gtgcccgata ccgtggctga
      781 atcaccaacc tggagatcgc cacaggatgc aacaacaccg ccttttttta tgatggtgtt
      841 ttgtgttatc ttgattgtga gcattccacc agaaacagtg atggagatga ctgaagggca
      901 ggatatggag gcgatcttgc cagttacatt gaatgtgcct gtttcccttg tgacagcaac
      961 ggtctgcgca gtgtatacac ccttgtccgc agttcctttg acttgggctg agtcgccgac
     1021 ttttagctca tcgcaagaaa ccgattttcc attgagcgta aaccttgcac tgccaataag
     1081 gattttcact gtctggctcg tcgcctgtaa caccatttcg cctgaagagc aatcgatcga
     1141 acttatttta cctgtggcaa caaaaccaac actgcccgta tttactttga catagctggc
     1201 atagtagctg ccatctgctc tctgttcgcc gctaacaatc accgggtctc caacttccag
     1261 gtcttcgcag gacagcgaac ctgtcggccc cattatcatg gccctcgaaa tatcaaattg
     1321 gatgtttccg gacgaggtcg caacaacaat tgcattcgga caatatgttg aaacaatttt
     1381 ccccgttgca gttatctttt ttacagagcc tgccccctgt gcggctatct tcgacgcaat
     1441 tatgcgaccc ccgttgtcaa ctccaacaac agtaacgctc atgttcggtt gcaaatcggt
     1501 acacggcgat accgcgccat tcacggtgat gatcgtcgag ccaacaatgg caacatcaaa
     1561 gaccttgcca agcgatgggg acgaaacctg tatcgaagaa tagcatgaaa ctgacgagat
     1621 gactcccgaa acagtgaccg atgcggcctg tgcggagcca atggggagaa gcccgaaaag
     1681 aagggcccct gcaagaatta cggacataaa ctttctcgca accatcttta tgcaccccta
     1741 acttaggatt tgaaacaaat acgaatcttt cttgttaaag gttcaaaaat tggagcgaca
     1801 aaatgaatgg aggcaagcta aattttctgt ttatttttgg tactcaagac cgtcatcgag
     1861 ccaggcgcca tccttgtggt atgccatgcc aagatactgg attcttgtat attcccagcc
     1921 cggcttcctt ctaaactcca ggctaagcgt gcgcctaccc ttgcttatcg ccagaaagcc
     1981 atattctttt tctgtgtcgt agggaacata gctggtgccg ccttcaaatt tcccccagcc
     2041 atcatccttg gctttttgca tataccattc gaaaatctct gctgggtcgc cacctgcaaa
     2101 cagtggcccg gaaacctcga ctccgaaaac cttttcggca accgtcttga catcaatcct
     2161 tttgccatag atttcgtttt caaatgcacc atcataaacg ggataatggg cggcaatttt
     2221 tggtataatt tcgtatgact tttttacttc aagtttttcg gtcttgacgg tactgccacc
     2281 gcaaccagaa agcaatgcaa caagaagaag gctacttgcc aaaaggcttg tcgctcgtgc
     2341 cctcataatt aaccctcacc cagcctggat aggctgtcag tgtccacacg tcaatcttca
     2401 tgaaagactc cttacctatt gcggtaatat gggccgtacc gtctgcttcc acaaagatga
     2461 gtgggtcctg gtcattgatt tcctcttttt ttgccaccct caagccagtg tctgtgagat
     2521 ggtcaacaat ccaggtgtaa acttcttcgg ccgttgcatt atgataggtt tcggcagtaa
     2581 ctttggtttt gccaatattt tcgttgtcct cacccacaat aaatgatctg gtggagtcga
     2641 atttggcatc tttgtatatc ggaacatagg gagagagctc gttcaatctt gtcttgtcag
     2701 ctttgaaggg tgcttcggtc actaccggct ctactatttc aatatccggc ttgtcgcctg
     2761 cttttagcgg attagcaagt ttgtctacat caagctcgcg tgatgcttgc gcgcaacttg
     2821 ccagggttgc aagaaggccc agcagaagcg caaaatttct gatatttttc attttccatc
     2881 ctgtggtttg gtgaacttat tgccaagata taaaatttct gtcctgctct gcactggatc
     2941 ataatttatc gtgaccgtaa ggacaaggtc acccttgatg caagtcagct tgccggtttg
     3001 aaacttttcg ctgtatggaa ggtcatagtt ggtcgcccag ccctttgccg caaactcgtc
     3061 cttgtaccac tcatatatgc gtaaagggtt cgaggaagtg taaagtgtac cggtgatttc
     3121 tctcacaagg tccgtctggc ctaccttttc attcaacacc ctcaatggat tcgctggatt
     3181 atcagaagat ggcctgaaca ccggaacata ttcgggaagt ggagatggaa ggccagtttt
     3241 gtcctcaggc tttggcaact cgaccatgga gatcgaacca gcagtgcaac cagagagcat
     3301 aactaccacc atagcgataa cggcaaaata cttcatgccc cctttgtacg caatgagtag
     3361 agactgtcaa ctaatcgcat attttgttga tttttgcttg catttggctt atttttgatt
     3421 ggttgatatc aaaggagtgg aaatgacaga caactatacc gcacaagata ttaaggtact
     3481 ggacggcata gaacacgtaa ggctaaggcc ggcaatgtac attggctcca cggacgagag
     3541 gggcttacac caccttgtgt acgaggtggt ggacaacgca attgacgagg caatgggtgg
     3601 atggtgcaag aacatcgagg taacgataca cccagatggt tccatttcag tcagtgacga
     3661 tggaagggga atacctgttg atcttcaccc gcaacttgga ataccaggtg ttacagtagc
     3721 actcacaaga ctcaacgctg gtggcaagtt tgacaaaaag tcatacaagg tgtcgggtgg
     3781 acttcacggc gtaggcgttt cagttgtaaa cgcgctgtct acctggctca tggtcacaat
     3841 aaaaaaagat ggcaaggtcc accgccagag gttccgcaag ggtgccccag aaacagacct
     3901 ggaaattacc ggcgagacaa aagaaacagg gaccatagtt actttcaagc ctgattctga
     3961 aatattctcc acggtcgaat tcgacttcaa gaccttaagc cacaggctaa gggagcttgc
     4021 atacctcaat tcggggctca ggattgccat aaaagatgag gtcaccggca aattcgagga
     4081 atacaagttt gatggtggac tcaccgaatt cgtggagtac ctctccgaag gcaaagaggc
     4141 aatccaccgc gagataatac attttaacga tacaaaggat gacatgtaca tcgaattcgc
     4201 catgcagtac acaacaggct atggcgaaac aattttctcc ttcgtcaatt cgatagccac
     4261 ggcagacggc ggagcgcatg taacaggcta ccatgctgca ctgaccaggg tgctcaacga
     4321 caaggcaaag ctccttggca tcataaaaac cgaggaagac agcttgtctg gcgaagacac
     4381 cagagatggc ctcgtatgcg tcctttcaat acgcatagcc gacccgcagt ttgaaggaca
     4441 aaccaagggc aggctcggca atatgaccgc caagtccggc gtcgagcgat acacccaaga
     4501 aaaactcagc tattacttcg acaaagacac ccagaccatt aaaaatatcc ttgaaaagtg
     4561 catcctggcc aaaaaagcca gggaggcagc caaaaaagcc agggaccttg taaggcgcaa
     4621 aagccttctg gaatcttcag tcctcccggg caagcttgca gactgctcgg agcgcgatcc
     4681 ctcaaaatca gaactgttca tcgttgaggg agaatcggca ggcggatcgg caaagcaggg
     4741 aagagacagg aaattccagg caattcttcc actgaaagga aagatcctca acgtcgagaa
     4801 ggcaaacctt aacagggtgc tctcaaatgc cgaggtcagg gcgctcataa catcgcttgg
     4861 ttgcggcatt ggcgaagatt tcgacattgc caggctcagg tatcacaaag tagtcatcat
     4921 ggccgatgct gacgtcgacg gtgcacacat cgcaacactc ctgctcaccc tcttctacac
     4981 acacttccgc cagctcatcg aaaacggcca tatctacatt gcccagccac cgctatacaa
     5041 aata
//
LOCUS       DAOSRI010000122         4998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15283_length_4998_cov_4.759053, whole genome shotgun sequence.
ACCESSION   DAOSRI010000122 DAOSRI010000000
VERSION     DAOSRI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15283_length_4998_cov_4.759053"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..734
                     /gene="efp"
                     /locus_tag="PK717_04600"
     CDS             171..734
                     /gene="efp"
                     /locus_tag="PK717_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR46973.1"
                     /translation="MITANDLRIGKTFEMEDGLWACVKFEHIKPGKGTAFVRVKIKNL
                     ENGSQVERTFRPEDKFKLAFLDYRKMQYLYRSGDEFFFMDQENYDQISVNEDDLGDAV
                     NYLKENMIIEVAYFKGKPVSVELPTFVEMEIIETEPGFKGDTAQNTFKPAKVESGYVV
                     QVPLFIEQGEIIRIDTRTGLYLERANK"
     gene            742..1173
                     /gene="nusB"
                     /locus_tag="PK717_04605"
     CDS             742..1173
                     /gene="nusB"
                     /locus_tag="PK717_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR46974.1"
                     /translation="MQRRQGRELALQMLFASSYFPENEREQGLLVLESSEPDSEVASF
                     AKTLFLGILKSRTEIDKIISKHLENWDLSRLWLVDKSILQIAIYELLFLKDSPPSVVI
                     DQAVRLAKKFGGDESGGFVNGILGAIFRSELNAKGKTEDKG"
     gene            1142..1414
                     /locus_tag="PK717_04610"
     CDS             1142..1414
                     /locus_tag="PK717_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOR46975.1"
                     /translation="MPRVKLRIRGRVQGVGFRHFAKTNAEKYGLVGHTKNRIDGSVEV
                     EVQGDRTSIQSFIGAMHIGPGYAEVGSVEITEIEENSYEKTFRVIF"
     gene            complement(1398..2456)
                     /locus_tag="PK717_04615"
     CDS             complement(1398..2456)
                     /locus_tag="PK717_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR46976.1"
                     /translation="MSASILSTSNEDQLISSLREYSLKELSSSCEFLVFVPFWGRHRT
                     EHPNADLVSKISALFRDLGHKVTLAWDHESFPDLRVPDGVKGQDLKRDHFIFSKGIGE
                     RKVSGPRRWDTSKPAFLQRIAVAECAYEAEAVLPILAPAPSTSFLVSGSITSFLSLLP
                     TQTIAKILLESTTSNVGSALAEVYSLLSFKTIGAVYDASQITDWPGIGKPDRKNFETK
                     IFSSDCVAGDAYISELFGWPSYLPQVVRASIDLGSGEGRLYNVDFDGRLLKGYFHIRR
                     MVSDKNTVFYPLAMVSRYYLKFNKDNCGFCHLCEHVCPNGSIKFDDGKMKVLGSCVKC
                     WACADSCPNFAIEIRRSP"
     gene            complement(2456..2836)
                     /gene="rpsI"
                     /locus_tag="PK717_04620"
     CDS             complement(2456..2836)
                     /gene="rpsI"
                     /locus_tag="PK717_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR46977.1"
                     /translation="MYRATGYRKTAIASVIMKPGEGKIEINGREFEHYFQTESHRKTV
                     LAPLEATDKVGKMDITVKVMGSGVNSQAEAVRHGIAKALLEVDAEFRPSLKKPGFLRR
                     DARKKERRKYGMRGARKARQYRKR"
     gene            complement(2844..3266)
                     /gene="rplM"
                     /locus_tag="PK717_04625"
     CDS             complement(2844..3266)
                     /gene="rplM"
                     /locus_tag="PK717_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR46978.1"
                     /translation="MDTVFLTGLEKKWFVVDAAGKPVGRVAVKVTRLLSGKEKPTWNP
                     HTDTGDFVIVVNANKVVVTGKKEQQKIYYHYSGYNGGLYEATFEKMMEKHPERVIEHA
                     VKGMLPQGRLGRAMFRKLKVYNGPAHPHAAQNPQAVKL"
     gene            3401..3592
                     /gene="rpmB"
                     /locus_tag="PK717_04630"
     CDS             3401..3592
                     /gene="rpmB"
                     /locus_tag="PK717_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR46979.1"
                     /translation="MSRVCEVCGKGPVTGHTISHSHIRAKRRWFPNLHSVRVQKGTSV
                     VRMKVCAKCLKANKVRVVA"
     gene            complement(3641..4414)
                     /locus_tag="PK717_04635"
     CDS             complement(3641..4414)
                     /locus_tag="PK717_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46980.1"
                     /translation="MKKCAFFLALLVCVSQVSLCVAADVTQSTQKPKTPFEELASIDA
                     LPPTIYLSNLDQKTNVAESVYLLTSVLDWSEQYRISATDSFWMRIWFRMERFVRYIRF
                     DFDKLSEIAAVKGFERLSNSKRWSIEFDMADLGKPVVGWQMLEMESFGLSEALSSNGI
                     QRTLREEVNSLLSQIASIKIEMTQKFADLKIEKEKTQIEDEVPLAYFLFALQHNLLPD
                     VENPTPLMLEETLTKGQALEFAMKAKQFLKGMVEDISNK"
     gene            complement(4411..>4998)
                     /locus_tag="PK717_04640"
     CDS             complement(4411..>4998)
                     /locus_tag="PK717_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46981.1"
                     /translation="AIFAFLHARRGEDVASITFGFSCLALFVSQVVLISIITKTGLPS
                     SLPSNYVLVYLLWYIAANYLLFGLGTFASIFMNGKIYVTIAVPVIVLLAILLGIGFAE
                     GIIPGLPRFESNGSWLWTISGVMFFASFLMYLLFWIQKKDPYRIKMAIAFLMIAICLF
                     LLTNILADTWIWYIAQALRLVGFAMIFFEVQTSYA"
BASE COUNT         1437 a         1125 c         1106 g         1330 t
ORIGIN      
        1 caacactgat catgttgacg ggaagtttgt caaggagcgg ctctccaatc gggaaattga
       61 ccatccatca aattgatact ctttgtgtgc cccgattgtc aacaaggaaa gggggttcgt
      121 tgacacggta tttgcttgcc tttatactct taacgcttta aggaggaata atgatcaccg
      181 caaacgatct aaggatagga aagacttttg agatggaaga tggcctctgg gcgtgcgtca
      241 aattcgagca cataaaacca ggaaagggca ccgcatttgt aagggtaaaa atcaaaaatc
      301 tggaaaacgg ttcccaggtt gagcgcacgt tcaggccaga agacaaattc aaactggcat
      361 ttcttgatta cagaaaaatg caataccttt acaggtcagg cgatgaattt ttctttatgg
      421 atcaggaaaa ttacgaccag atttcagtaa atgaagacga cctgggagac gccgtcaatt
      481 atctcaaaga gaacatgata attgaggtcg catatttcaa gggaaaacct gtatcagtcg
      541 agctgccaac ctttgtcgag atggaaataa ttgagaccga accaggtttc aagggtgaca
      601 ctgcccaaaa tacattcaaa ccagcaaagg ttgagtcggg atatgtcgtt caggtcccac
      661 ttttcataga gcaaggcgag atcatcagga tcgacacaag gaccggcctc tatttggaaa
      721 gagccaacaa gtaaccgcca gatgcagagg cgacaaggaa gagaacttgc attgcaaatg
      781 ctttttgctt cgtcctactt tccggaaaat gagagagaac agggactcct tgtacttgaa
      841 tcatcggaac cagattccga agtcgcctct tttgcaaaaa cgcttttcct ggggattctg
      901 aaatccagga ccgagataga caaaattatc tcaaaacacc tggaaaattg ggacttgagc
      961 aggctatggc ttgttgacaa gagcatactc cagatagcta tatacgaatt acttttcctg
     1021 aaagactccc cgccgtctgt tgtcattgac caggcagtca ggcttgcaaa aaagtttggc
     1081 ggcgacgaat ctggaggttt tgtcaacggg atactcggtg caattttcag gagcgagctg
     1141 aatgccaagg gtaaaactga ggataagggg tagggtacag ggcgtcggtt ttcgccactt
     1201 tgccaaaacc aatgccgaga aatacggcct cgttgggcat acaaaaaaca ggattgacgg
     1261 gagcgttgaa gtagaagtgc aaggggacag gacaagcatt caaagtttta ttggtgcaat
     1321 gcacatagga ccgggatatg ccgaagttgg ctcggtggaa atcacagaaa ttgaggaaaa
     1381 ttcctacgaa aaaaccttca gggtgatctt ctgatctcta ttgcaaaatt ggggcaagaa
     1441 tcggcacagg cccagcattt cacgcacgaa cccaaaactt tcatttttcc atcatcaaat
     1501 tttattgacc cattgggaca aacatgttcg cacaaatgac aaaatccaca attgtcctta
     1561 ttaaatttga ggtagtacct cgaaaccatt gccaaagggt aaaaaacagt gtttttgtct
     1621 gataccatcc gcctgatatg gaagtaaccc ttcaaaagtc ttccatcaaa atccacatta
     1681 tagagtcttc cctcaccact cccaaggtct attgacgccc tcaccacctg cggaagatac
     1741 gatggccatc caaataattc tgaaatatag gcatctcctg cgacacaatc agaagagaat
     1801 attttggttt caaaattttt tctgtctggt tttccaatgc cgggccagtc tgtgatttgt
     1861 gaagcatcat aaactgcgcc tattgttttg aatgacaaaa gtgaataaac ctctgcaagc
     1921 gcagaaccaa cgtttgaagt tgtgctttca aggagtattt ttgctattgt ctgggttggc
     1981 aaaagcgaca aaaaagatgt tatggagcca gaaacaagaa acgacgttga aggggcaggt
     2041 gcaagaattg gcagaacagc ttctgcctca taagcacatt ctgcaactgc aattctctgc
     2101 aaaaaagcgg gtttgctggt gtcccatctc ctgggaccgg aaacttttct ctcaccaatt
     2161 ccctttgaaa aaataaagtg gtctcttttt aaatcctggc ccttgacgcc atctgggacc
     2221 ctaagatcag ggaaagattc gtggtcccaa gccagggtga ccttatgacc caaatcccta
     2281 aaaagagcac ttatttttga taccaaatcg gcatttgggt gttcggtcct gtgcctgccc
     2341 caaaagggga caaaaacaag gaactcacaa gatgaagaaa gctccttgag ggaatattcc
     2401 ctcaaggagc ttataagttg gtcttcgttt gaagtagaga ggattgaagc agacattaac
     2461 gtttgcggta ttgtcttgct ttcctggcgc ccctcattcc atattttctt ctctcttttt
     2521 tgcgggcgtc tcttcttagg aatcctggtt ttttgaggct tggcctgaat tcagcatcaa
     2581 cctcaagaag tgctttggct atgccgtgac gtacagcttc ggcctggctg tttacgccag
     2641 atcccatgac cttgacggtg atgtccatct tgccgacttt gtcggttgcc tcaagcggag
     2701 ccagtactgt cttgcggtgg gactctgtct ggaagtagtg ttcaaattcc ctgccgttga
     2761 tttcaatctt gccttcacct ggcttcatta tgacgcttgc aatggctgtt ttacgatatc
     2821 cagttgccct gtacatattc ctcctacaac ttcactgcct gcgggttctg tgcggcatgg
     2881 ggatgagctg ggccattgta gacctttagc tttctaaaca ttgcccttcc aagtcttccc
     2941 tggggtagca ttccttttac ggcatgctcg atgactcttt ccgggtgctt ctccatcatc
     3001 ttctcaaaag tggcttcata gagtccgcca ttatatccgg aataatgata ataaatcttc
     3061 tgttgttctt tcttgccggt aacaaccacc ttgttggcat taacaactat aacaaaatcg
     3121 cctgtatcgg tgtgcgggtt ccaagtcggc ttttctttgc cggagagcag gcgtgttacc
     3181 tttacggcga ccctgccaac cggttttccg gccgcatcca ctacgaacca ttttttctcc
     3241 aggccggtca aaaatactgt atccatagca atcctcctac aaagcaacaa ttttatagcc
     3301 aaaccctatt atgtcaagga aaaccaattt tccattttaa ccctttacat gtttgttccc
     3361 ctttgctaaa tttgacattc gtattcaagg aggtagaaac atgtccagag tttgtgaagt
     3421 atgcggaaaa ggcccggtca ccggccacac tataagccat tcccacatca gagccaaaag
     3481 gcgctggttc ccaaacctcc acagcgttag ggtccagaaa ggcacttcgg tagtaagaat
     3541 gaaagtctgc gcgaaatgtc tgaaagccaa caaagtcagg gtagtggcct aggcaaaatt
     3601 ttttccataa caaaaagccc cggggtctcc tggggctttt ttatttgttc gaaatgtcct
     3661 ctaccatacc tttaaggaac tgtttcgctt tcattgcaaa ctcaagggcc tggcctttgg
     3721 ttaatgtttc ttcgagcatt aatggtgttg ggttctccac atcaggcaaa aggttgtgct
     3781 gaagggcaaa caaaaaatag gccagaggaa cctcgtcctc tatctgggtt ttttcttttt
     3841 ctatcttcaa atctgcaaac ttttgggtca tctcgatttt tatcgaggct atctgcgaca
     3901 ataaagagtt tacttcttca cgaagggtcc tctgtatgcc atttgaggag agggcttcgg
     3961 aaagaccaaa actttccatt tcaagcatct gccagccaac tacaggcttt ccaagatcag
     4021 ccatatcaaa ctcaatgctc cacctctttg aattcgagag cctttcaaaa ccctttacgg
     4081 cagcaatttc actcaatttg tcaaaatcaa accgaatata tcgtacaaac ctttccattc
     4141 tgaaccatat tcgcatccaa aaggagtcgg tcgctgatat cctgtattgc tcggaccagt
     4201 caagaacaga tgttaaaagg taaacgctct ctgcaacatt ggttttttgg tcaagatttg
     4261 agaggtatat tgtcgggggt aaagcatcaa tactggccag ctcttcaaat ggtgtttttg
     4321 gtttctgggt gctctgggtg acatcagctg caacacacag ggacacttgg gaaacacaca
     4381 caagcaatgc aagaaaaaaa gcgcattttt tcatgcgtag ctggtttgta cctcaaaaaa
     4441 tatcatggca aagccaacaa gcctcaatgc ctgggcaata taccaaatcc atgtatccgc
     4501 caaaatattg gtcagcagga acaagcaaat tgcaatcatc aggaaggcta ttgccatttt
     4561 tattctgtac gggtcttttt tctgtatcca aaaaaggagg tacattaaaa agcttgcaaa
     4621 aaacattact ccagagattg tccaaagcca tgagccatta ctttcaaatc tcggcaggcc
     4681 aggtattatt ccctcggcaa aacctatgcc aagaagtatg gcaagaagga caatgactgg
     4741 aacagcaatt gtgacatata ttttcccgtt catgaagatg gaagcaaagg ttccaagtcc
     4801 aaaaagaaga taatttgcgg caatatacca caggagatag acaagcacat aattcgatgg
     4861 gagtgatgat ggaagtcctg ttttggtgat tatggagatc aacactacct gggagacaaa
     4921 gagggcaaga caggaaaaac cgaaagtgat cgaggccaca tcttcccctc tcctcgcatg
     4981 gagaaaggca aaaatggc
//
LOCUS       DAOSRI010000123         4985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15330_length_4985_cov_3.670385, whole genome shotgun sequence.
ACCESSION   DAOSRI010000123 DAOSRI010000000
VERSION     DAOSRI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_4985_cov_3.670385"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1251
                     /locus_tag="PK717_04645"
     CDS             220..1251
                     /locus_tag="PK717_04645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR46982.1"
                     /translation="MNIQIRKVENEELRTTLFLQYKAHATMMLHEAEPEMKPISDEMA
                     MQWLDDKYEVSIRNYAFEGEKIVGDAEIYLRKPDDPEYELNKGLGYIFGYVEPKYRNR
                     GIGTSLAKKAFLDIKDSCVHTISSHSYSEEGNRFLQKFGVPSSVFSDMRLELDGFDWN
                     IVDGWLKIAEKSENNWKIELHTKITDQFIDDVSDLSFATAVEFKKMNNQETIDLREAE
                     KKKWQDSKEFLEKFRQYRCFLVKDANGVVIGFTEGGIDNENTYRFIQYVTAVDAGHRG
                     SGIGKLLKALMLDHIRQNNPQVKMITTGNNDLNAPMLGINSRLGFKKHKTQISYKICV
                     EEALKRLGI"
     gene            complement(1339..1668)
                     /locus_tag="PK717_04650"
     CDS             complement(1339..1668)
                     /locus_tag="PK717_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46983.1"
                     /translation="MKTNKEFFDKLVKIVEDGGVSAFAFMETLKRARSPVYKGVCGGS
                     GMQPVLELLYSLVGLEPDDSRVSRLATQIRNLPDYNNPNLITYNFEQSNAIADKIEKL
                     IQEQKGK"
     gene            complement(1836..2870)
                     /locus_tag="PK717_04655"
     CDS             complement(1836..2870)
                     /locus_tag="PK717_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOR46984.1"
                     /translation="MANIDYAVKFLEEICKTPSPTGFAMAAIDRCEREARSLGFATRR
                     NNKGGLLVDVPGKDASFRRLVSGHVDTLGAMVRSIDSNGWLKFTPIGGYMMQTVEGEY
                     CQIFTRSGKTYTGTCLTTDPSVHVHPNARELERKPENMVIRLDERVCSKEDTEKLGIS
                     AGDFIAWDPRTIVTESGFIKTRHLDDKAGTACIFCALQAISEEKITPAHPFTAIITNY
                     EEVGHGASFVPEANEILAVDMGAIGLDLSTDEYCVSICAKDSSGPYDYALTNQLVDLA
                     KANKIKYAVDIYPFYGSDASAALHGGHDLRAGLIGPGVQASHSMERTHKDAIEATSKL
                     LVEYLKTEKV"
     gene            complement(2967..4703)
                     /locus_tag="PK717_04660"
     CDS             complement(2967..4703)
                     /locus_tag="PK717_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOR46985.1"
                     /translation="MKKLLTFALILSLVSPIWIVAKASECDTTCDIMKMPLSKVKNIA
                     MSNGGKIPPSMVHSTIRPTKHLGDQKVLVLPMEFPDCRWTIAAGSIREKFISQTQRSL
                     LNFYEISSYGKYRPTYGEKGIPEWMMMPKPYNDYEDMDSLFNDAFATAIKYGIDPLEY
                     DEDGNGFPDLTIVIWAGNSYTVGGDKPGDFTMPMEIGNIISIGEDVRVGGFPLITLLH
                     EYFHAMGELYDLYDYSYTSDPVGGWDLMAAGVWDGYCGINSYQRWKANWIEPETITEP
                     GTYEVDDLNGDGPHKMYKIPLPGSDVEWLLVENRQRHGGDGYFQGCPGNGMIVYHVDD
                     RREYKHLFNTRTKEWKTVGIEVLDPGGSPLHKTAMYGDNYGKVAISADSIPNTLPYEK
                     NDSKKTLRIDKISSVGEKMTFRLSYDTPQVPIMQVLDTLSFGRVVKGEMASQDLTFYN
                     IGPKKLQAMLKSDSQWINIDRTSFIGNEEPINVTINTASLKYGFNSGYIKYDGNAGTG
                     RVKVVVDVAPIPGDVNLDGVVDDADVELFAKYFNSSLGDPNYYKDADFNNDQKVDLMD
                     FLMMSKNYKSKI"
BASE COUNT         1267 a         1118 c         1169 g         1431 t
ORIGIN      
        1 cgttgaccca aggtcaaacc ccaatgccga gttgttcttc aaaagggaca tggcaatcct
       61 ggggcgccac ttcaagaggg aggaagaggc aagggaaatt gccgattcgc tctggctgga
      121 gtgggagacc gggaagattt gaggaaaaca tccagggtcc ctcacaaagc aggggccctg
      181 gtctactcga aaattgatga aaacaacatg ggagacaaaa tgaacattca aatcagaaaa
      241 gttgaaaacg aagagttaag gacaacattg tttttgcagt acaaagcgca tgcaaccatg
      301 atgctccacg aggcggagcc tgaaatgaaa ccgatatcgg acgagatggc gatgcagtgg
      361 cttgatgaca agtatgaagt atccataaga aattatgcct ttgaaggcga gaaaattgtt
      421 ggcgatgcgg aaatatattt aagaaaaccg gatgatccag aatatgaact gaacaaaggt
      481 cttggttata tatttggtta tgtggagccc aaatatagga acagaggcat tggtacaagt
      541 ttggccaaaa aagcttttct ggacatcaaa gacagttgtg tccacacgat tagctcacac
      601 tcttattcgg aagagggcaa caggtttctt caaaaatttg gtgtgccttc ctcggttttt
      661 tctgacatga ggctcgagct tgacgggttt gactggaaca ttgttgacgg atggcttaag
      721 attgcagaaa aatctgaaaa caactggaaa attgaactgc acaccaaaat aacggaccag
      781 tttatcgatg atgtcagcga cctgtctttc gccacagcgg ttgaattcaa gaaaatgaac
      841 aaccaggaaa caatagacct gagggaggca gagaaaaaga agtggcagga ttccaaggag
      901 tttcttgaaa aattcaggca gtatcgctgt tttctggtca aagatgcaaa cggggtggtc
      961 atcggcttta ccgagggtgg catcgacaat gaaaacactt acagattcat acagtatgtg
     1021 acagctgttg atgcaggaca tcgcggaagc ggcattggca aactgctcaa ggccctgatg
     1081 ctcgaccaca tcaggcagaa caatccccag gtcaagatga tcacaacagg caacaatgat
     1141 ctgaatgccc cgatgcttgg gattaactcc agactgggtt tcaaaaaaca caagacccaa
     1201 atcagctaca agatttgtgt tgaggaggct ttgaagaggt tgggaatata aaattgtagt
     1261 tttttctaga gattagatag aattgagaaa aaatatgccc ctgagtgata aacttggggg
     1321 catttcaagc tcaacgtttt atttcccctt ttgttcttga attaattttt caattttatc
     1381 tgcaatagcg tttgattgct caaaattgta tgttatgagg ttcggattat tgtaatcagg
     1441 taaattcctt atttgtgttg caagccttga tacccgtgaa tcatctggct ctaaaccaac
     1501 aaggctataa agtaattcca gcacaggttg catacctgac ccgccgcata cccctttata
     1561 gacagggctt ctagctctct tcaaggtttc cataaaagca aatgccgata ctccaccatc
     1621 ttcaacaatt ttcaccaatt tatcaaagaa ttccttgttt gttttcaatg ttgtcctcct
     1681 aatattttgt attaatttat tccatattcc cttggggatt tatgaaaaca ttatatcata
     1741 aaatataatt ttgtcaagta gctaaaataa cacaataagg cgcatctgat aggtagaaca
     1801 ctctaattat acaaataata tgagtattct catttttaca ctttttcggt cttcaaatac
     1861 tccaccagca atttcgaagt ggcctcgatt gcgtccttgt gggtgcgctc catcgaatgg
     1921 ctggcctgaa cacccggccc gatgaggccg gccctcaggt catggccgcc gtggagggcc
     1981 gcagatgcat cgctcccgta gaacgggtag atgtccaccg cgtatttgat tttgtttgct
     2041 tttgcaaggt cgaccagctg gtttgtcagg gcgtagtcgt aggggccgga agagtccttg
     2101 gcgcagatcg agacgcagta ttcgtcggtt gagaggtcga gcccgattgc gcccatgtcc
     2161 acggccagga tttcgttggc ctctgggaca aagcttgcgc catggccgac ttcttcgtag
     2221 ttggttatga ttgccgtgaa ggggtgtgct ggcgttattt tttcctcgga gattgcctga
     2281 agggcacaaa aaatacaggc cgttccagcc ttgtcgtcca gatgtctggt ttttatgaag
     2341 ccagattctg tgacgattgt ccttgggtcc caggctatga aatctcctgc cgagatgccc
     2401 agtttttcgg tgtcttcctt ggaacagacg cgctcgtcga gccttatcac catgttctcg
     2461 ggttttctct cgagttccct ggcgtttggg tggacgtgca ccgaagggtc ggttgtcagg
     2521 catgttccgg tgtaggtttt cccgctcctt gtgaaaatct ggcagtactc gccttccact
     2581 gtctgcatca tgtagccgcc gattggcgta aatttcagcc agccgtttga atctatggag
     2641 cgcaccattg cgccgagggt gtcaacatgg ccggagacga gcctcctgaa ggacgcgtcc
     2701 ttgccgggaa catcaaccag cagcccaccc ttgttgtttc ttcttgttgc aaacccgagc
     2761 gatcttgcct cgcgctcgca cctgtctatt gccgccatgg caaagcctgt cgggcttggg
     2821 gtcttgcaga tctcttcaag aaattttacc gcgtagtcaa tgtttgccat gtttcacctc
     2881 ttttgtctgc caattctatc attgccggtg gaaacaaaaa agggcgacct tttgggccgc
     2941 ccttttttcg atgactggtg agtcaatcaa atttttgact tgtagttttt tgacatcatg
     3001 aggaaatcca tgaggtcgac cttctggtcg ttgttgaagt ctgcatcctt gtagtagttt
     3061 gggtcgccaa gcgagctgtt gaagtacttc gcaaacagtt cgacatctgc atcgtccacg
     3121 accccgtcga ggtttacatc tcccgggatc ggggccacgt caacaactac cttcaccctt
     3181 ccggtgccgg cgttgccgtc gtatttgatg tatccactgt tgaagccgta cttgagtgaa
     3241 gccgtattaa ttgttacatt tatcggctct tcattgccaa taaagcttgt tctgtcaatg
     3301 ttgatccatt gggagtcaga cttgagcatt gcctggagtt tttttgggcc gatgttgtag
     3361 aatgtgaggt cctggctggc catttcacct ttgactaccc ttccgaaaga cagggtgtcc
     3421 agcacttgca tgattggaac ctgtggggtg tcatagctta gcctgaaggt catcttttca
     3481 ccgactgaag agatcttgtc aatcctcaaa gtctttttgg agtcgttttt ctcatatggc
     3541 agagtgttgg gtatcgagtc ggcgctgatg gcgacttttc catagttgtc cccgtacatt
     3601 gctgttttgt ggagtggtga tccacctgga tcaagaacct ctatgccaac agttttccac
     3661 tcttttgttc ttgtgttgaa aagatgtttg tactctctgc gatcatcgac atggtaaaca
     3721 atcatgccat ttccgggaca accctggaaa tagccatctc ctccgtgcct ttgcctattt
     3781 tcaacaagaa gccactcgac atcggagcct ggaagcggga ttttgtacat tttgtgtggg
     3841 ccatcaccat tcaggtcatc gacttcataa gtccctggct cggtgattgt ttctggttct
     3901 atccagttgg ctttccatct ctggtaggag ttgatcccgc aatatccgtc ccatacacct
     3961 gcggccatga ggtcccagcc accaacgggg tcagatgtgt atgaataatc atacagatcg
     4021 taaagttcgc ccattgcatg gaaatattcg tgaaggagtg taatcaacgg aaaaccacca
     4081 actctgacat cttcgccaat gcttatgatg ttgcctattt ccattggcat tgtgaaatcc
     4141 ccaggcttgt ctccacctac agtgtaggaa ttgccggccc aaataactat tgtcagatca
     4201 gggaacccgt tcccatcctc atcgtattcg agtgggtcga tgccatattt tattgctgtt
     4261 gcaaaggcat cattgaagag ggagtccatg tcttcataat cattgtatgg ttttggcatc
     4321 atcatccact ctggaatgcc tttttcaccg taggttggcc tgtatttgcc atacgaggat
     4381 atctcgtaaa aattgaggag gcttctttga gtctgtgaga tgaatttttc ccttatactg
     4441 cctgcggcaa tggtccatct gcaatcgggg aattccattg ggagaacaag gaccttttgg
     4501 tcaccaagat gtttcgttgg ccttatggtc gagtgcacca ttgatggcgg tattttccca
     4561 ccattcgaca tggcgatgtt ttttaccttg gaaagtggca ttttcatgat gtcacaggtt
     4621 gtgtcacact ctgaagcttt ggcaacaatc catatcggtg aaaccaaaga caggatcagc
     4681 gcgaaagtca ataatttttt catttacaaa ctagaatacc acgggttgat ggcatgtaaa
     4741 caaaaagaca aaacctgaaa gaagcgtgtt tttatctaaa tgatcacgct taccccccta
     4801 gtacttttta agaattgatt agaatggttt aactattgta aatattactt tctgccaata
     4861 tttctcgcaa ggagcattaa gtccaaccaa tcaataattc catctttgtt taggtcagcc
     4921 tccttcttgt acccctcttg accttcgctt tttccaaagc aagcaaccaa ttcgtcaaaa
     4981 tcttt
//
LOCUS       DAOSRI010000124         4944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15486_length_4944_cov_3.360196, whole genome shotgun sequence.
ACCESSION   DAOSRI010000124 DAOSRI010000000
VERSION     DAOSRI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_4944_cov_3.360196"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PK717_04665"
     CDS             <1..1122
                     /locus_tag="PK717_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46986.1"
                     /translation="YVGSTRVAWSPSYWRKLEDGGMDTILYLFCRYLSVPGTSVGQAI
                     AQARVEFAKQYFFGDIEDPIESAQMNIYNFNLYGDPSVCLIKGDIGGSVEILEPSISA
                     SPGEVAKWEANISGSDLTGNIFQVIPDKQDMSWAVPEVVIAGNKLVLTAEIPSSVPNG
                     KRFWILHQKRGDLSSQIPIVLNIVPKPEASFGFSSGDGAIIPFSRVKSSLNLTQKTKY
                     LEVTLKYDPDKIKFVSIGQPSKKSDVFSFFDNHFGLAYIRISGASEVLPLISFRVMKF
                     VGKTSIDVSKIVAMTDRPEALAIQSKSFEIAIGEKELWISRADFDRSGKVDSFDLALL
                     LSRIGCTKQDPKYDPVYDLDDNGLIDFIDYSLAFKRISG"
     gene            complement(1112..2620)
                     /gene="purH"
                     /locus_tag="PK717_04670"
     CDS             complement(1112..2620)
                     /gene="purH"
                     /locus_tag="PK717_04670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOR46987.1"
                     /translation="MSYVLISVHDKTGLEPLARAITQTGYKILSTGGTADHLRKLGFQ
                     ITEVASYTGSPEVLGGRVKTLHPKIHAGILCRRDNPKDMEELGKMTAESIDIVICNLY
                     PFEKTIASQDCTLEKAIEEIDIGGVTLLRASAKNFQHVTILSDPSQYSEFIERLSLGR
                     FDIEYKKSLAIRAFRTTNAYDGAISAYLEGPIKDHLGVPETIHTRQVMPLRYGENPHQ
                     QAGLYSVGNNEWPIASLQGKELSYNNILDIDAALSTLLEFDPTCAVIVKHLTPCGVAI
                     GTSMKEAYLRALECDPVSAYGGIVAINDTIDEEAAIEVGKLFVEVIIAKDFSKEALSI
                     FAKKKNTRLVTYNKDLPRAKFAIRSTMFGTLFQQPDLSLADSFKIAGETPVSEHDLEQ
                     LTFAMAVAKHVKSNAIVLAKDFATVGVGAGQPNRVGSVKIASSQAGEKAKGSYLASDA
                     YFPFADSIEHAASFGVKAIIEPGGSIRDDEVIAAANKLGIGLVFSGQRHFNH"
     gene            complement(2617..3228)
                     /gene="purN"
                     /locus_tag="PK717_04675"
     CDS             complement(2617..3228)
                     /gene="purN"
                     /locus_tag="PK717_04675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOR46988.1"
                     /translation="MKKIAVLVSGRGSNLKALYGAIEQGKLEAEICLVVSNKSKAPAL
                     EFCRDKNIPYQVCKLKDFESREVFFDHLAGILAPSNADLVVLAGFMMLVTPNFIRRFK
                     NRVINIHPSLLPSFPGLDAQQQAFDYGVKVSGCTVFIVDEGCDTGPILIQKPVEVLET
                     DTEETFADRILSVEHDTLWRACKIMLDGRVSVSGRKTLIKEDQ"
     gene            complement(3228..4229)
                     /gene="purM"
                     /locus_tag="PK717_04680"
     CDS             complement(3228..4229)
                     /gene="purM"
                     /locus_tag="PK717_04680"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOR46989.1"
                     /translation="MSKAYESTGVSIDASDRFVEKIKEAAKRTIIPGVLGGIGFFGGF
                     FEVPKGYSNPVMVSGADGVGTKVKIAQALGKHDTVGIDCVAMNVDDIVCSGAKPLYFL
                     DYLAVNRLDEEVSYQIIKGVAEGCVQAGCALIGGETAQMGDIYQPNEYDLAGFAVGIV
                     EKSKIIDGSKIKKGDIIVGLPSSGLHSNGYSLVRKIASKYSLDSKFDGIDHFGLALLE
                     PTKIYAQYIVKIAQAVELHGISHITGGGLADNLPRIMGSGKAVVKRNSLPKIPIIEFL
                     IKEAKLSDEDAFRTFNMGIGMALFVPEEEASKTMSLYPGSVAIGTVEGDGEPSFGLV"
     gene            complement(4213..>4944)
                     /locus_tag="PK717_04685"
     CDS             complement(4213..>4944)
                     /locus_tag="PK717_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOR46990.1"
                     /translation="CLNFGNPTNPEIYYEFEQSVLGMCDALRALNIPVISGNVSFYNE
                     SPSGAIKPTPVVVMVGKARAGKINKPDFQNTGDVIVLLGETLPELGGSALFEMLTGET
                     FGTPPMIRINAEVSLSKTLLAATSEGIIRSATDLSEGGLAQALVECVCNSGLGAQIAL
                     KATGIHITEQLFSESNARALVTVSPDCVSRLTEICAQNETQIKILGVVGGKTLSVTGS
                     FDIPVQELKELYENSLRKVMDEQGL"
BASE COUNT         1212 a         1163 c         1166 g         1403 t
ORIGIN      
        1 tatgttggtt caaccagggt tgcctggtca ccatcatact ggaggaaact cgaagatggc
       61 ggaatggaca ctattttgta cctcttttgc aggtaccttt ccgttccagg cacatccgtg
      121 gggcaggcaa tcgcacaggc gagggttgag tttgccaaac aatatttctt tggtgacatc
      181 gaggacccaa tcgagtcggc ccagatgaat atttacaact tcaatctcta tggcgatcca
      241 tcggtttgcc tgataaaggg tgatattgga ggaagcgtcg aaatacttga accaagcatc
      301 agcgcctctc ctggagaagt ggcaaaatgg gaggcaaata tctccggcag cgatttgact
      361 ggcaatatct ttcaggtgat cccggacaag caggacatga gctgggccgt cccggaagtc
      421 gtgattgctg gaaacaagct tgttttgaca gcagaaattc catcatcagt tcctaacggc
      481 aagagatttt ggatactcca ccagaagagg ggcgacctgt cgtcgcaaat accgatagtt
      541 ctcaatattg tgccaaaacc ggaggccagc tttggatttt catcaggaga tggcgcgata
      601 atcccgtttt caagggtcaa gtccagcctt aatttgacac agaaaacaaa atatcttgag
      661 gtcacactga aatatgatcc tgacaaaatc aagtttgttt ccataggtca gccatcaaaa
      721 aaatcagatg ttttctcgtt ttttgataac cattttggtc ttgcatacat caggatttca
      781 ggagcttccg aggtactgcc gctgataagc ttcagggtga tgaaatttgt aggaaaaaca
      841 tcaatcgatg tatcaaaaat tgttgcaatg acagacagac cggaggcttt agccatccag
      901 tccaaatcgt ttgaaatagc aattggggag aaagaacttt ggatctcgag agccgatttt
      961 gatagatctg gtaaagtgga ttcttttgac ctagcgcttc ttctgtcgcg aatcgggtgc
     1021 acaaaacagg acccaaaata tgatccagtt tatgatctgg atgataacgg cctgatagat
     1081 tttattgatt acagccttgc tttcaaaaga atcagtggtt gaagtgtctc tgtcctgaga
     1141 aaaccagacc aatcccaagt ttgtttgccg ctgcaatcac ttcatcgtct ctgatggaac
     1201 cgcctggttc aatgattgct tttaccccaa agcttgccgc gtgctcaatc gaatctgcaa
     1261 acgggaagta tgcatcagag gcaagatagg agcccttggc tttttcgccg gcctgtgatg
     1321 aggcgatttt cacggaacca accctgtttg gttgaccggc accaacacct actgttgcaa
     1381 aatcttttgc cagaactatt gcattggatt ttacatgctt ggccaccgcc atggcaaaag
     1441 tgagctgttc gagatcgtgc tcggatactg gagtttcgcc agcaattttg aatgagtcag
     1501 ccaggctgag atcgggttgc tggaaaagag ttccgaacat tgttgatctg atggcaaatt
     1561 ttgccctggg aaggtcttta ttgtatgtca ccagtcttgt attttttttc ttcgcaaaaa
     1621 tagagagtgc ctctttgctg aaatcttttg cgataatcac ttcgacaaat agttttccaa
     1681 cctcgattgc tgcttcctcg tcaattgtgt catttatagc aacgataccg ccatacgcgg
     1741 aaactgggtc gcattccagt gccctaaggt aggcctcttt catgcttgtg cctatcgcca
     1801 ccccgcatgg agtgaggtgt ttgacaatta cagcacatgt tggatcaaac tccagcagtg
     1861 tcgagagcgc ggcatcaatg tcgaggatgt tgttgtagga gagctctttc ccttgtagtg
     1921 atgcaattgg ccactcattg ttgccgacgg aataaagccc ggcctgctgg tgagggtttt
     1981 caccatacct gagaggcatc acctgccttg tatggattgt ttcaggaaca ccaagatggt
     2041 cttttatagg accttcaaga tatgctgata ttgcgccatc gtatgcgttt gttgttctaa
     2101 aagctctgat cgcaagagat ttcttgtact caatgtcaaa cctgccaagt gaaagcctct
     2161 cgatgaactc actatattgt gaaggatcac taaggattgt gacatgctgg aagttttttg
     2221 cgcttgcgcg gaggagtgtt acaccgccga tgtctatctc ctcaatggcc ttctcaagag
     2281 tgcagtcctg tgatgcgatg gttttttcaa aaggatagag attacagatc acgatatcta
     2341 tcgactcggc agtcattttg ccaagctctt ccatgtcttt ggggttgtct ctcctgcaga
     2401 gtattccggc gtggattttg ggatggagcg tcttgaccct gccgccaagg acctcaggtg
     2461 agcccgtata gctggcaact tcagtaatct ggaagcctag ttttctaagg tggtctgctg
     2521 ttccgcccgt tgaaaggatt ttatagcctg tctgtgtgat tgccctagcg agtggctcaa
     2581 gtcctgtttt atcgtgcact gaaatgagaa cgtagctcat tgatcctcct ttattagcgt
     2641 ctttcttcca gaaacgctta ctctcccatc aagcatgatt ttgcatgccc tccagagtgt
     2701 gtcatgctct accgaaagta ttctatcggc aaacgtctct tcggtgtctg tttcaagaac
     2761 ttctacaggc ttctggatga gtatggggcc tgtgtcgcaa ccctcatcaa ctatgaatac
     2821 cgtgcacccg gagaccttta ccccgtaatc aaatgcctgt tgttgtgcat caaggcccgg
     2881 gaaggacggc agaagcgaag gatggatatt gataaccctg tttttgaaac gccggatgaa
     2941 attgggtgtt acaagcatca taaatccggc gagtactacc aggtccgcat tggatggggc
     3001 gaggatgcct gccagatgat cgaaaaagac ttctcttgac tcaaaatctt tcagcttgca
     3061 aacctgatag gggatattct tgtccctgca aaattcaagc gcaggtgctt ttgatttgtt
     3121 tgaaaccaca agacaaattt ctgcttctag cttaccctgt tctattgcgc cgtagagcgc
     3181 cttgagattg gagcccctgc cagatactag aactgcaatc tttttcatca gacaagcccg
     3241 aaagaaggct ccccgtcacc ctcgactgtt cctatggcta ccgagcctgg gtagagggac
     3301 attgtttttg aggcttcttc ttccggtaca aacaatgcca tgccgattcc catgttgaag
     3361 gtcctgaatg cgtcttcatc tgaaagcttg gcctctttga taaggaactc gattatcggg
     3421 atttttggga ggctgtttct ttttacgacc gcttttccag agcccatgat tcttggaagg
     3481 ttgtcagcga ggccaccacc cgttatgtgg gagataccgt ggagttcaac agcttgtgct
     3541 attttgacaa tatattgggc atatattttt gttggctcga gaagcgcaag accaaagtgg
     3601 tcaatcccgt caaattttga atccagggaa tattttgatg caatcttcct gacaagcgaa
     3661 tatccgtttg aatggagtcc tgatgatggc agtccaacta ttatatcacc tttttttatc
     3721 tttgatccat cgatgatctt tgatttctcg actattccaa ctgcaaaacc tgcaaggtca
     3781 tattcgttgg gctggtagat gtcacccatc tgggcagttt ctccaccaat tagggcgcat
     3841 ccagcctgaa cgcaaccctc ggccacacct tttattatct ggtatgacac ttcttcatcg
     3901 agcctgttta cggccagata atcaagaaaa tacagtggtt ttgcgcctga gcagactatg
     3961 tcatcaacgt tcatggccac gcaatcaata ccaaccgtgt cgtgtttgcc aagtgcctgg
     4021 gcgatcttca cttttgtccc gacaccatcg gcgcctgaga ccatgacggg gttcgaataa
     4081 ccctttggga cctcaaaaaa accgccaaaa aaacctattc cacccaagac accgggaata
     4141 attgtccttt ttgcggcttc ttttattttt tcgacaaacc tgtctgaggc gtcaatagaa
     4201 actcccgttg actcataggc cttgctcatc catcaccttc ctcagggaat tctcatagag
     4261 ctctttcagc tcctgcactg ggatatcaaa tgaacctgtc accgatagtg ttttgccgcc
     4321 aacaacgcca agtattttta tctgtgtttc gttctgggcg catatctcgg tgagccttga
     4381 aacacaatct ggagatacag tcaccagagc ccttgcgttg ctctcggaga aaagctgttc
     4441 tgttatatgg atgccagttg ccttcagggc gatttgcgcg ccaaggcctg agttgcacac
     4501 gcactcaacg agtgcctgtg caagaccgcc ctctgaaaga tcagttgccg aacgaataat
     4561 gccttcacta gttgctgcga gaagcgtttt tgaaagtgaa acctctgcat taattctgat
     4621 cattggcggt gtaccaaaag tctctccggt aagcatctca aagagtgccg atccgccgag
     4681 ttctggcaat gtttcaccaa gcagcacgat gacgtctccg gtgttctgga agtctggctt
     4741 gtttattttt ccagcccttg ccttgccaac catgactacg acaggggttg gttttatggc
     4801 cccgcttgga ctttcgttat agaagctgac gtttccggag atgaccggga tgttgagggc
     4861 cctcaaggca tcgcacatcc caaggacact ctgctcgaat tcgtagtaga tttcaggatt
     4921 ggttgggttg ccgaaattca ggca
//
LOCUS       DAOSRI010000125         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15495_length_4942_cov_3.738694, whole genome shotgun sequence.
ACCESSION   DAOSRI010000125 DAOSRI010000000
VERSION     DAOSRI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_4942_cov_3.738694"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK717_04690"
     CDS             <1..453
                     /locus_tag="PK717_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46991.1"
                     /translation="KYIAIAGFRGRLWLLAQDDTIAEFDSQAGTIIKETGLKLTNPKK
                     GNFGARLDTLGNVICASWHDNSLTMTEVVSFDPNSGKILEFAEKNSTFQITNGQLIEK
                     KDDGFYCLDLSSGSAIWSVKTNRGASLVWADEDGVIITSNGSTMVFGE"
     gene            457..1458
                     /gene="hypE"
                     /locus_tag="PK717_04695"
     CDS             457..1458
                     /gene="hypE"
                     /locus_tag="PK717_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOR46992.1"
                     /translation="MKDFVTLAQGGGGTESKKLVDVITSFIGNDVLAPFDDAGVFGVE
                     KGRLAFTTDGFVVKPIFFPGGNIGRLAACGTLNDLAVMAARPLAISLSLVIEEGFPLE
                     KLVQIMESFKTDLGGTKVVCGDTKVVEKGSVDGVFMTTSGIGLQMDNCHPSGSSLEPG
                     DVLIVTGSLGRHGACIMACRDELGFFGQIQSDVAPLWPMLEKLFVGIGENIHACRDIT
                     RGGFSAIVNEFAQSSRVGIDIDEALLPIDPDVSGMCGALGLDPLTLACEGRALVSVRK
                     GFEEKALEILKGSEQGSGACIVGSVSETHSQLVHLKTRIGGARVLDPPAGELLPRIC"
     gene            complement(1455..2480)
                     /locus_tag="PK717_04700"
     CDS             complement(1455..2480)
                     /locus_tag="PK717_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOR46993.1"
                     /translation="MSARKGINLIGTGDILYPPWEKEWRDFLSKPSKSIGVVPTTEIS
                     VHFPFGGAKKAFHFILVFSNFEVVDKVRERLLKYGRINDFARPDLYIEPARLEEEIFS
                     SDSESIIIPAHIFTPYFSILGRRGVEKFEDLRISKSPCETGISADPAMCATLKTLEGV
                     SIVSFSDAHSPETIGREATIIENGIPLKKSLMSPLMTIECCPEFGKYHVTGHKPCGVG
                     LREDEDFEICPKCGKKMTLGVAQRIGRLEKSANPKTQPFKKIIPLNDLLAFSLGLSSP
                     TAKSKKLAEKAIESIGSELYILLEAGEGELEKILPEKTTEMILKVRSGNIRIRPGFDG
                     EYGRIVS"
     gene            2461..2922
                     /locus_tag="PK717_04705"
     CDS             2461..2922
                     /locus_tag="PK717_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR46994.1"
                     /translation="MPFLADISTILAGVVVLEQDMAYLPWRCKSAIDKFGLWNRFVIM
                     GDSEGIGQRIRVLRQKLNYTQIQVEKATGIPPSNLSEFENGKLIPTIQTLFKIAKFFG
                     VSVAYLLGEGDLEGLKVNVSLLSELSEDGKKQIQDFIDFVVQREMEKEKEQ"
     gene            3647..4591
                     /locus_tag="PK717_04710"
     CDS             3647..4591
                     /locus_tag="PK717_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR46995.1"
                     /translation="MKWASIYAGTIILSVLSCVLVNFVTVSYPTRAHEFSMLMSTGTG
                     RLSYLLSEQIAYVKSEEMIYSESATQLLEATKQLESKDKTIREKDEIAPQYKTLKEIF
                     NSKPYRELATNPQLSDQGSVISAKAATILQRIDSTTHIFAQAERITDNLYWLRTHTPN
                     CYPVPDGETRDHNPGFGYRIHPIWGTFDNHTGIDIEGKLGEPIYAVADGVVTRAKLYG
                     GYGRCIDILHRKDEGGIVSRYGHCDEIVVKEGDMVKRGDLIGYVGSTGTSTGPHIHFE
                     ITIDNRPTDPDEYIRNVNISQKYLKKEVPAQQKASGGK"
     gene            4668..>4942
                     /locus_tag="PK717_04715"
     CDS             4668..>4942
                     /locus_tag="PK717_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46996.1"
                     /translation="MLMSGKGSTKRGLGFKILIISISFILLGSLVTTFVITNKIAAEK
                     RLAEIEGIVRKQATTTKSIAMMFLTRQMVEMATDFPIYPIKDFTSRK"
BASE COUNT         1372 a          990 c         1190 g         1390 t
ORIGIN      
        1 aaatatattg ctattgccgg attcagggga agattatggt tactggcgca agatgacacg
       61 attgcagagt ttgattcaca ggcaggcaca ataataaagg aaactggttt gaaactgaca
      121 aacccgaaga agggcaattt tggggccagg cttgatactc tggggaatgt catctgtgcc
      181 tcatggcatg acaattcact tacgatgaca gaggttgttt catttgaccc aaacagcggg
      241 aaaattctcg agtttgctga aaaaaattcc accttccaga taacaaatgg tcagctgatt
      301 gagaagaagg atgatggttt ttattgcctt gatctgtcct ctggcagtgc tatctggtca
      361 gtaaaaacaa acaggggtgc cagtcttgtc tgggccgatg aggatggagt aatcattact
      421 tcaaatggtt cgaccatggt ttttggagaa tagcagatga aagattttgt aacacttgcg
      481 cagggcggtg gcggcaccga atcaaagaaa cttgttgacg tgattacatc gtttattggc
      541 aatgacgtgc ttgcgccgtt tgacgatgca ggcgtttttg gggtggaaaa ggggcggctc
      601 gcttttacaa ccgatggatt tgttgtcaaa cctattttct tcccgggtgg caatattgga
      661 aggcttgcag cttgcggaac tttgaacgat ctggccgtga tggccgccag acctttggcc
      721 atctcgctct ctcttgtgat tgaagagggg tttccgctag aaaaactggt gcagatcatg
      781 gagagtttca agaccgatct gggtggaaca aaagtggttt gcggcgacac aaaagttgtt
      841 gaaaagggtt ccgttgatgg ggtattcatg acaacttcgg gcatcggcct ccagatggat
      901 aattgccatc caagcggctc cagtcttgaa cctggtgatg tattgattgt caccggttct
      961 cttggaaggc acggggcttg cataatggcc tgcagggacg agcttggatt tttcggccag
     1021 atacagtctg acgttgcccc actgtggccc atgctggaaa aactttttgt cgggattggt
     1081 gaaaatatcc atgcctgccg ggacatcaca aggggaggat tttctgcgat agtcaacgaa
     1141 tttgcccagt cctcccgtgt tggcattgat attgatgagg cattgttgcc aattgaccct
     1201 gacgtatctg gtatgtgtgg agcgttgggt cttgatccgt tgacgcttgc ctgcgagggc
     1261 agagcgcttg tttcggtcag gaaagggttt gaagaaaaag cactggaaat cctgaagggc
     1321 agcgaacaag gctctggtgc ctgtatcgtt ggctctgttt cagaaaccca ttcccagctg
     1381 gtgcacctta aaaccaggat tggcggcgca agggtgcttg atccaccagc tggagagctc
     1441 ctgccaagaa tctgctagga aacaatccta ccgtattccc cgtcaaaacc cggcctgatt
     1501 cttatgttcc ccgatcttac tttcaatatc atttcggtgg tcttttcggg caatattttt
     1561 tcaagctcac cctcgccagc ctccagaagg atgtaaagct cggaaccaat tgattcgatc
     1621 gccttttctg ccagtttttt tgattttgcg gttggagagg aaagcccaag cgaaaaagcg
     1681 agcaaatcgt ttagaggaat gatttttttg aatggctgtg tttttgggtt tgcgcttttt
     1741 tcaagcctcc caatcctttg ggctacacca agggtcatct ttttgccgca tttggggcat
     1801 atctcgaaat cttcatcttc cctgagtcca acaccacaag gtttgtgacc ggttacatgg
     1861 tatttaccga actctggaca acactctatc gtcatgagtg gcgacatgag tgattttttc
     1921 agcggaatgc cattttcgat gattgttgcc tccctgccta ttgtctccgg gctgtgtgca
     1981 tccgagaagc tgacaatcga tactccttca agggtcttga gggtggcgca catggccggg
     2041 tccgcagaaa tgcctgtttc gcatggcgat tttgatatcc tcaggtcttc gaatttttca
     2101 acaccccgtc gaccaagtat ggaaaaatat ggcgtaaata tgtgtgcagg aatgattatt
     2161 gattcagaat cggaagaaaa gatttcctcc tcaagacgtg ctggttctat gtaaaggtca
     2221 ggtctggcaa aatcgttgat tctgccatat tttaacaatc tttctctgac cttgtcgacg
     2281 acctcgaaat ttgaaaaaac caatatgaag tgaaatgctt ttttggcgcc accaaaagga
     2341 aaatgcaccg aaatttcagt tgtggggaca accccaatgc tttttgaagg ttttgagaga
     2401 aagtctctcc actccttttc ccatggtgga tataggatgt cgcctgttcc aattaggttt
     2461 atgccttttc tggcagacat ttccacaata ctggcaggtg ttgttgttct tgaacaggat
     2521 atggcatatt tgccgtggag atgtaaatcg gctattgata agttcggatt gtggaataga
     2581 tttgtaatta tgggtgattc tgagggcatt ggacaaagaa taagggtttt aagacaaaag
     2641 ctcaactata cccagattca ggttgagaag gccaccggta ttcccccatc aaacctttcg
     2701 gagtttgaaa atggcaagct tataccgact atccagacac tgtttaaaat tgccaaattc
     2761 tttggagtca gtgttgccta tcttttggga gagggtgacc ttgaaggtct caaagtcaac
     2821 gtgagcctgt tgagcgagtt gtccgaggat ggtaaaaaac agatccagga tttcatagat
     2881 tttgtagttc aaagggagat ggagaaagaa aaggagcaat aggcctcact taaaattcag
     2941 tataaaaggg ctggttgctg gggtatgctt atttttttgc gagattagca atagcacaga
     3001 taagcattga tcataaattt ctcaaattcc ttacaagaat tgttactgcg catgagctta
     3061 gctttttttt gctcatgaaa ctgatgagta ttgatttgca cgagataaag gtgaattttt
     3121 tgtgtgtaac tttcttttgc gagagaggat cgtaattggt gtttttgaga tgcgattctg
     3181 aatgtaaaaa aatggttttg ttattggaat agcctaagtt tctcgtagta ttgaagattt
     3241 ttatagttag aaaactataa ggatacaaaa ttgataccga aaatgctaag aggcatttga
     3301 tgcagattag ataattattt tattgactaa gtgtgctata atatcccgaa aataaccgtg
     3361 tgctgactgt tcccattatt gcacacccca atctctttac gtaccaaatt catcattttg
     3421 aaacactcca caatattgca agtccatgtt tcgcttgaca cacaaaatga ggcaaataca
     3481 ataacacccg tttgagaaat tcattgtttg cctcaaatat taccaaacaa tgaatttttt
     3541 gttgtgtttt actattccat tgatgaggtt aggtgtgaat cagaaacaaa cgaaacggtt
     3601 atcggtaatt ttgttgccgg gccacgagag ggttttttca tttagtttga aatgggcgag
     3661 tatttatgca gggactataa tactctccgt tctttcgtgc gtgcttgtca actttgtcac
     3721 cgtttcctat ccaaccagag ctcatgaatt cagtatgttg atgtctaccg gcacaggcag
     3781 gctttcttac ttgctttcag agcagattgc ctatgtcaaa tcagaagaaa tgatatattc
     3841 agagtcggca acccaactcc tcgaggccac aaaacagctt gagtccaaag ataaaacaat
     3901 cagggaaaag gacgaaatag ccccgcagta caagaccctc aaggagatct tcaattcaaa
     3961 accatatcgc gagcttgcca ccaatcctca actttcggac cagggctcag ttatttctgc
     4021 caaagcagca acaatactcc aaagaattga cagcacaacc cacattttcg cccaggcaga
     4081 gcgtatcacg gacaatcttt actggctcag gacacatacg ccaaactgct acccggtccc
     4141 agatggtgaa acaagggacc ataatcctgg ttttggatat agaatccatc caatttgggg
     4201 gactttcgat aaccacacag gaatcgatat tgaaggcaaa cttggtgaac cgatttatgc
     4261 agttgctgat ggtgttgtta ccagagcaaa gttgtacggt ggttatggtc gctgtataga
     4321 cattctccat cgcaaggacg aaggtggaat tgtatccaga tatggccatt gcgacgagat
     4381 tgttgtaaaa gagggcgaca tggtcaagag gggtgacctg ataggctatg taggttcaac
     4441 aggaacctcg acaggcccgc acatccattt tgaaataacg atagacaata ggccaacaga
     4501 cccagatgaa tatatccgta acgtaaacat aagccagaaa tacctcaaaa aggaagtccc
     4561 ggcccagcaa aaagcgagcg gggggaaatg atgatgctcc aggtctggaa aaaacaaaat
     4621 agggcgcttt ttgggggttt tctccagcag gagaaaagga ggagcaaatg ctgatgagcg
     4681 gaaagggtag tacaaaaagg ggacttggtt ttaaaatttt gattatttca atatctttta
     4741 tcctgttggg ttctttggtg acaacttttg taataacaaa caagatagcc gctgaaaaaa
     4801 gactggctga aattgaaggt attgtcagaa aacaggccac cacaacaaaa tctatagcca
     4861 tgatgttttt gacacgccag atggtcgaaa tggccacaga ttttccaatt taccccatta
     4921 aggatttcac aagcaggaaa ca
//
LOCUS       DAOSRI010000126         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15609_length_4915_cov_3.533128, whole genome shotgun sequence.
ACCESSION   DAOSRI010000126 DAOSRI010000000
VERSION     DAOSRI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15609_length_4915_cov_3.533128"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="PK717_04720"
     CDS             <1..1754
                     /locus_tag="PK717_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOR46997.1"
                     /translation="NPTSESWEGAKQGDELSTNFKIKTASSSWAEVRLDDGSLLRIGE
                     NTELEIVSLYINPMMGIRSARWLMKGYGSVYVNTGETGSIYDIITSGFVASATRGTMK
                     VDGDGRGNFLVKVPNSNSNATVTIGNMITRVPSLEQARIDSSNKLKMEKSLVDESDTW
                     NAQMDAPDLKLFYEKTTFEEKEAILGKTNPLNKILLDGQEVAKSSSDGTFEIEIKLPI
                     GKIKYTIRSIDPAGRYVEQQFEIERLEKSDHQLIITNPQEGATITEDKVAIKGIAEGS
                     VSLTANGKPIQLSQDKFEIEFQPVEGPNKLNFVSKDKNGKVVTKTLTFIKHKPMPQAQ
                     LAITSPDEGTQTESESIIVSGHADTEYVSVGSRMARVEKGIFSITVPLDFGRNSIVVM
                     ARSEQKKTASQTVIVYRSKPNSTKPEIAINSTSPLTNKSSIVIIGACKNAKIVQFQNQ
                     TVEPSENGKFEFIAPLNEGENTLTVFAQSFDGGTASASINITKDTTLPDLSKLKATRV
                     IGETKVVISGRLEHNCTLLVNGLAISQKDIFAGADFDSIIHVATSISGDTVVLVGRDP
                     AGNESRLELKIEQSRGR"
     gene            1732..2568
                     /locus_tag="PK717_04725"
     CDS             1732..2568
                     /locus_tag="PK717_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46998.1"
                     /translation="MSRAGAGSLRKRPFLSGTIIAFIIIAIEAILLNVLPGIMARLIP
                     NDLRWTEFPTAFITLVNASLAFFFSYQTFSAFDLQDYKRKIWRFVMLSMGAWVIGHFL
                     SIIIRFGFMVDMTRPPNVADYLGYLWMLPLLLVALSRQYGLVKTNSPPNKLMKLGLAV
                     VLLLFAGTILLVSPFFSPKFITLPERLVSLWYIGFSFAIMVVAVSLLSEISSGLISVP
                     WKFIIMAVFFFCLSFVLFYFIGTNNLDSGSHLVKTLQAILSQAGTILIGTASFIESRL
                     FG"
     gene            2597..3097
                     /locus_tag="PK717_04730"
     CDS             2597..3097
                     /locus_tag="PK717_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR46999.1"
                     /translation="MKKFVVIFVCCFVILGFWSGSGYCATVKNDAFKVALEAAGLSYE
                     NISWDASKLSRIDDADPFRLQWFNEIWANPLRVPKIAKQITDRIPIFATNISKELDYI
                     FFYGTSRTCGSVAALMEDYLPLLEMAKEKPFYNSIKKVYQNMGKNSTQNCLHTKGNSR
                     KKRQMP"
     gene            3049..4638
                     /locus_tag="PK717_04735"
     CDS             3049..4638
                     /locus_tag="PK717_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47000.1"
                     /translation="MSAYEGQLTQEASDALAEYVLSADWLFRERKKALEAGDLTEETF
                     RKLLVANTGDEDTGDFTYRLGKRFDMKQMMYACCRIVFSTQKLKGAFGGGVPLPEGKN
                     VFPTPFGDIILNGSRNDDLYEGNNHLLVVDVGGNDTYEGTFGATSSLYNCASVVIDVS
                     GNDTYKDPYGHSPNQGAGVLGCGILADLWGNDTYEAANNCQGAGVFGIGMLYDYTGND
                     TYSSIDFSQGAGCFGIGAMVEYDGDDVRKCFFGGQGYGATMGFGLSYDRSGNDTYISD
                     DENIIHPSAQNPSHNTSMSQGCGNGRRADITDGHSMSGGCGVLVDSEGDDTYKCGVFG
                     QGVGYWFGVGIFTDTKGNDTYEGLWYVQGATAHFAIAVFRDTEGNDSYTAKQATSLGV
                     GHDFSISYHIDEGGDDKYNCYMADPKDDKIKQYGGLMIGCGNETGMGFFFNIGGDDVY
                     DAKSDTMYGYANVAAPLAPDTIRNDMLCLGLFIDIGGEDAYGKNFCKQNGSWVRRNGD
                     RPEMVIGIGLDAEDGELPILE"
     gene            4642..>4915
                     /locus_tag="PK717_04740"
     CDS             4642..>4915
                     /locus_tag="PK717_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47001.1"
                     /translation="MDKMTQKLLASEEPSIRYKALVNILGEPRDSKKAVSASMEVKNS
                     PLVKKLLSEKPKNVHVSRGVGVYDKWLGDHWVLSLLADLGYPKGDKE"
BASE COUNT         1473 a         1103 c         1197 g         1142 t
ORIGIN      
        1 acaaccccac aagtgaatcc tgggaaggtg cgaaacaggg cgatgagctt tcaaccaact
       61 ttaagatcaa gactgcttca agctcgtggg cagaggtaag gttggatgac ggttcactgc
      121 taagaatcgg ggaaaacaca gagctggaga ttgtctcact ctacatcaat cccatgatgg
      181 gtatccgttc agcaaggtgg ttgatgaagg gttacggaag tgtctatgtc aacactggcg
      241 aaacaggctc aatatacgac ataatcactt ctggctttgt ggcctcggca acaagaggga
      301 cgatgaaggt cgatggcgac gggaggggca acttcctggt caaagtgcca aattcaaaca
      361 gcaatgcaac agtgacaata ggtaacatga tcacaagagt accgtcactt gaacaggcaa
      421 ggattgattc ctccaacaag ctcaaaatgg aaaagtccct ggtggatgag tctgatacat
      481 ggaacgccca aatggacgca ccggacctga aactgtttta cgagaaaaca acatttgaag
      541 aaaaagaggc catcctcgga aaaacaaacc ctctaaacaa aatccttctg gatggccagg
      601 aagtggccaa gtcttccagt gatggcacct ttgaaataga aatcaagctc cccatcggca
      661 agataaaata caccattagg tcaattgatc cggcaggaag atatgtagag caacaatttg
      721 agatcgaaag gcttgagaaa agcgaccatc agctaataat caccaaccca caagaggggg
      781 caaccataac cgaagacaag gttgcaatca aaggaatcgc tgaaggttcg gtgtctttga
      841 ccgcgaacgg aaaaccaatt cagctttccc aggacaaatt cgaaattgaa tttcagcctg
      901 tagaagggcc aaacaaactg aattttgtat caaaagacaa gaacgggaaa gtggtcacaa
      961 aaacgctcac atttataaaa cacaaaccca tgccgcaggc ccagcttgca ataaccagcc
     1021 cagatgaggg cacgcaaaca gaatccgaaa gcataattgt ctctggccat gcagacactg
     1081 aatacgtttc agtagggtca agaatggcac gcgttgagaa gggcattttt tcaataactg
     1141 taccactcga ttttggaaga aactcaatcg ttgtaatggc aaggagcgag cagaaaaaaa
     1201 cagcctcaca aactgttatt gtttacaggt caaaacccaa ctccacaaaa cctgaaattg
     1261 ccataaacag cacttcgccc ctcacaaaca aaagcagtat cgtaataata ggggcctgca
     1321 aaaatgcaaa gattgtccaa tttcaaaatc agactgttga accaagcgag aatgggaaat
     1381 tcgaatttat tgccccgctc aatgaaggtg aaaacaccct gacggtcttt gcacagtcct
     1441 tcgatggcgg aacagcctcg gcctcaataa acatcacaaa agatacaacc ttgcctgacc
     1501 tctcgaaatt aaaagcgaca agggtcattg gtgaaaccaa agtcgtcata agtggaaggc
     1561 tggaacacaa ttgcacgctc ctcgtcaacg gcctggcaat atcccagaaa gatatctttg
     1621 cgggggccga ttttgattcc ataatccacg ttgcaacatc aatctctgga gacaccgttg
     1681 tgcttgtcgg gagagacccg gctggaaacg aaagcagact ggagctaaaa attgagcaga
     1741 gcaggggcag gtagccttag aaaaaggcct tttctttcag gcacgataat cgctttcata
     1801 attattgcaa tagaggcaat tcttttaaat gttttgccag gaataatggc aagacttatt
     1861 ccaaatgacc ttcgctggac agagttccca acagcattta taacgcttgt caatgcctca
     1921 ctggcctttt tcttctcata ccagactttc agcgcatttg accttcagga ttacaagaga
     1981 aaaatatgga gatttgtaat gctctccatg ggcgcctggg ttatcggcca ctttctgtcg
     2041 ataataatca gattcggatt catggtggac atgacaaggc cgccaaatgt ggctgactac
     2101 cttggatact tgtggatgtt gccgcttctt ttggtggcac tttcaaggca atatgggctg
     2161 gtcaaaacaa actccccacc aaacaaactg atgaagctgg ggcttgctgt cgtgcttctt
     2221 ttgtttgctg ggacaatcct gctggtctca ccattttttt cgcccaaatt cataacattg
     2281 ccagaaaggc ttgtttctct gtggtatata ggtttctcgt ttgccataat ggttgtggct
     2341 gtatcactcc tgtctgaaat tagctccggc cttatatctg tcccttggaa attcatcatc
     2401 atggcagtgt ttttcttctg cttgtccttt gtactcttct atttcatagg cacaaacaat
     2461 ctggacagcg ggagccacct tgtgaaaacc ttacaggcaa tactctccca ggcaggaaca
     2521 attctgatag gaacagcatc ttttatcgaa tcaagattgt ttggatagca tttttatcac
     2581 agcaaggggg catgtgatga agaaatttgt tgtcattttt gtctgctgtt ttgttatttt
     2641 aggcttctgg agtggttccg gatattgtgc aaccgtgaag aacgatgcct tcaaggttgc
     2701 gctggaagca gctggacttt cttacgagaa catctcatgg gatgcctcga agctctccag
     2761 gatagacgat gctgatcctt tcaggttgca atggttcaat gaaatctggg caaacccgtt
     2821 gagagtaccc aaaatagcaa agcagatcac cgacaggata cccatttttg caacaaacat
     2881 cagcaaagaa cttgattata tcttcttcta tggtacctcc agaacatgcg ggtcagttgc
     2941 ggccctcatg gaggactatc tcccacttct ggagatggcc aaagaaaaac ctttctacaa
     3001 ctctataaaa aaagtctacc agaatatggg aaaaaactcg acacaaaatt gtctgcatac
     3061 gaagggcaac tcacgcaaga agcgtcagat gccctagcgg aatacgtatt gagtgcagac
     3121 tggctcttca gagaacgcaa gaaggccctt gaagcaggag atctgacaga agaaaccttc
     3181 agaaaactgc tcgtcgccaa tactggtgac gaagacaccg gagatttcac atacaggctt
     3241 ggcaagcggt ttgacatgaa gcagatgatg tatgcttgtt gcaggattgt cttttccaca
     3301 caaaaactca agggcgcctt tggtggtggc gtgccactcc cagaagggaa aaacgttttc
     3361 ccaacaccat ttggcgacat aatattgaat ggatcaagga acgacgattt gtatgaaggc
     3421 aacaaccatc ttctggttgt tgatgttggt ggcaatgaca cctacgaggg cacatttggc
     3481 gcaacatcgt ccctctacaa ttgcgcatcg gtcgtgattg acgtgagtgg caacgatacc
     3541 tacaaggacc cttatggtca ctccccaaac cagggtgcag gtgttcttgg ttgtggaatc
     3601 ctggccgacc tctggggaaa cgacacctat gaggccgcaa acaattgtca gggagcaggc
     3661 gtcttcggca tcggcatgct gtatgattac acgggaaatg acacatattc ctcgatcgac
     3721 ttctctcagg gtgctggctg tttcggcatt ggggccatgg tggaatacga tggtgacgac
     3781 gtcagaaaat gtttctttgg cggccagggt tacggggcaa caatgggctt cggactctcg
     3841 tatgacaggt ccggaaacga cacctacatt tcagacgatg aaaacataat ccatccatct
     3901 gcacaaaacc cgtcccacaa cacaagcatg tcgcaaggct gcggaaatgg caggagggca
     3961 gacataacag acggccattc aatgtccgga ggttgcggtg tcctggtaga cagcgagggc
     4021 gacgatacat acaaatgcgg tgtctttggc caaggtgtcg gatactggtt cggtgtgggg
     4081 atttttactg acaccaaggg caatgacaca tacgagggat tatggtatgt tcaaggcgca
     4141 acggcacact ttgccatagc ggtcttcagg gacaccgagg gcaacgacag ttacactgcg
     4201 aaacaggcga cctcgctggg tgtgggacat gatttctcca tatcctacca catcgatgaa
     4261 ggcggggacg acaaatacaa ctgctacatg gcagacccca aagatgacaa gataaaacaa
     4321 tacggtggcc tgatgattgg ctgtggaaat gaaaccggaa tgggattctt tttcaatatt
     4381 ggcggggacg acgtctatga cgcaaagagt gacacaatgt atggctatgc aaacgtcgcc
     4441 gcaccacttg caccagacac catcagaaat gacatgctgt gcctcggatt gttcattgat
     4501 ataggtggag aagacgccta cggaaaaaat ttttgcaagc aaaatggcag ctgggtcagg
     4561 agaaacggtg acaggccaga gatggtaatt ggaataggcc tggatgcaga agatggcgaa
     4621 ttgccaatac tggagtaaaa aatggacaaa atgacgcaaa aactgctggc ctcggaagag
     4681 ccatcaatca ggtacaaggc acttgtaaac atacttggtg agccaaggga ttctaaaaaa
     4741 gcagtgtctg caagtatgga ggtgaaaaat tctcctctgg taaaaaaact gctctccgag
     4801 aaaccaaaaa acgtgcatgt ttcaagggga gtcggagtct atgacaaatg gctcggggac
     4861 cattgggtgc tgtcactgct tgcagacctc ggatatccaa agggcgacaa ggaac
//
LOCUS       DAOSRI010000127         4912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15615_length_4912_cov_3.733169, whole genome shotgun sequence.
ACCESSION   DAOSRI010000127 DAOSRI010000000
VERSION     DAOSRI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_4912_cov_3.733169"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..607
                     /locus_tag="PK717_04745"
     CDS             122..607
                     /locus_tag="PK717_04745"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOR47002.1"
                     /translation="MRENLVDELLETVKRVDPSFFEKLVIDLLLKMGYGGSRAEAGRV
                     VGKTGDGGIDGVIDEDKLGLDKIYIQAKRWENTVGRPIVQAFVGSLASNHSKKGIFIT
                     TSDFTKDAREYIEKIDSRISLVDGRKLVELMIDHGLGVSTSNTYEIKKVDRDYFPEDL
                     F"
     gene            complement(599..1183)
                     /gene="pth"
                     /locus_tag="PK717_04750"
     CDS             complement(599..1183)
                     /gene="pth"
                     /locus_tag="PK717_04750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR47003.1"
                     /translation="MEKWLIVGLGNPGLKYAHTRHNVGFDAVDALAKSLKTRISRMSC
                     GAKIARLEFDEMKFVIAKPLSFMNLSGGPVGCLANANEVPSDHWIIIHDDIDLPSGAL
                     RIRKSGSNGGHKGIASIIQETGKSDFYRIKIGVGRPETKDEVVDFVLSRTKDEGLGEA
                     IKKAAQACLFLIEEGYQAAGNKFNFVPKDQDELE"
     gene            complement(1310..2650)
                     /locus_tag="PK717_04755"
     CDS             complement(1310..2650)
                     /locus_tag="PK717_04755"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOR47004.1"
                     /translation="MEILPFKGVHYSKQAGNIQDLITQPYDKITPKMQEEYYKTSPYN
                     AIRVELGKDLQGDDENYNKYVRAEWYTKLWMAENIFERDQEPCIYVYNQEYIHPDTGK
                     TVTRMSFIALGKLHEYSEKIVYPHEHTLSGPKQDRLMLMRSTKTNTGLVLMFYRDNED
                     AINKVLSGKTGSGPLFDAVDEYKTHHKIWKISDPATIKKVQDLMDPKITIICDGHHRY
                     ETALNYRNEMREKNPGFTGKEGWNYRMIAYVNMADPNLYVFPTHRLVRDVPNFDGAKL
                     LEQLKGNFDVAEVEVSGSAVDAARKLVEMMGKNVDKHAFGYFPKGSKKMYMLTLKDLS
                     IADSIPVEKSNDWKRLDVTILHKLILEKQLGIDDAKLAAQTNVDYERYVPEAVEPVAK
                     GTHQCVFLMNPTLTTQVEDVTKHFETMPQKSTDYFPKVQSGLVIQYFPDKETVK"
     gene            complement(2657..3571)
                     /locus_tag="PK717_04760"
     CDS             complement(2657..3571)
                     /locus_tag="PK717_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOR47005.1"
                     /translation="MAKILISDPIAKAGVDLLQKEGHEVVVKKMTPEELVSSIGDYDA
                     IIVRSATKVTADVIKAGSKLRIIGRAGVGLDNVDSNAAKEKGIKVVNTPAATSISVAE
                     LAIGHMLAASRWIGYGTATMKEGKWEKKVMEGVELYGKTLGLIGVGRIGRETAKRAMA
                     FGMEVIAFDPYVSDPGMQGIHMVSKDELLAKADFISLHIPLTPETKNILDKQDFDKCK
                     KGLVLVNCARGGTVNEEALYNALKEGKLFAAGIDVFEKEPPEGVNKLASLPNVALTPH
                     IGAATEEGQTRAGVQLAEKIAEFFKEGK"
     gene            complement(3592..4734)
                     /locus_tag="PK717_04765"
     CDS             complement(3592..4734)
                     /locus_tag="PK717_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOR47006.1"
                     /translation="MKTLYKFLLAPGPTPLPEEVRLEGAREITHHKHPVAKKMFRKVT
                     DDMKWLLGTKYDSYLLASSGSGAMETAVQNLVNTGDKVIVINAGNFGGRWVKINKAYG
                     ANVIEIKKDWGDMATPAELEAVIKENPDTVAVFCQLSETSTGACSDTAGFAKIVSKTN
                     AILVVDGVSGIGVVPLKMDEWNVDVVVGGSQKGMMLPPGMAFICMNEKAWKLVENNKQ
                     HKFYFDLKLFQKKLVDNETPWTPPIGIIYQADKAFEMMKAEGLENVWARSTKLAKGIR
                     TAMKAMNLELFAKCPGDAVTTVKIPDGVDGKKVTSVIRDKYGITIAGGQGDFSGKIFR
                     LGHMGYMDMYSELACLAALELTLKELGHKFEIGSGVSAFMKEMAQE"
BASE COUNT         1076 a         1174 c         1162 g         1500 t
ORIGIN      
        1 gttaaaaagt tataggcatt aatatcaagg cccaaaagac aattatccta gacaaacaga
       61 gggtattgtt gattgtgggt ctataacccc cgaagaagag atagatcagg cctttagaga
      121 tttgagggaa aatcttgtag acgagttact tgaaacagta aagagggtcg atccgtcatt
      181 ttttgaaaaa cttgttatcg atctgctctt gaagatgggt tatggtggtt cgagggccga
      241 ggctggcagg gtggttggaa aaactggcga cggtgggatt gatggtgtaa ttgatgaaga
      301 caagcttggc ttggacaaga tttatattca ggccaagaga tgggaaaata ccgttggaag
      361 accgattgtt caagcttttg tgggcagtct ggctagcaat cactcaaaaa aaggcatttt
      421 tatcactaca tccgatttca caaaagatgc cagggaatac atagaaaaaa ttgacagccg
      481 gatttcgctt gttgacggca gaaagctcgt tgagttgatg attgatcacg ggcttggtgt
      541 cagcacgagc aacacatacg agatcaaaaa agtcgacagg gactatttcc ctgaagacct
      601 attctagttc atcctggtct tttggtacaa aattgaactt gttgccggcc gcctggtaac
      661 cttcctctat taggaaaagg caagcctgtg cggctttttt tattgcctct cctagtcctt
      721 catccttcgt ccttgaaagg acaaaatcaa ctacctcatc ttttgtctcc ggccttccga
      781 cgcctatttt gatcctgtaa aaatccgatt ttccagtttc ttgtattatg gatgcaattc
      841 ccttgtgtcc gccatttgat ccgcttttcc tgatcctgag ggcccctgac gggaggtcta
      901 tgtcgtcatg gatgattatc cagtggtcgg aaggcacctc gttagcattt gcaagacacc
      961 cgactggccc accagacagg ttcatgaaag aaagaggttt tgcaatcaca aatttcattt
     1021 catcaaattc cagcctggca atttttgccc cgcaggacat ccttgaaatc cttgttttca
     1081 gcgattttgc gagggcatcc acagcatcaa aacccacatt gtgccgtgtg tgtgcgtatt
     1141 tgagtccggg atttccaagt cccactatga gccatttttc catacggaaa cattatctac
     1201 aaaaaactcc ggcagtcacg attaaaatat ttacacaaat tgcaatttgc gaccaaataa
     1261 aaaacggccc ttttgggggc cgttttttat ttgtctggtt aatatttggc tattttacag
     1321 tctctttgtc tgggaagtac tgaattacaa gccctgactg gaccttcggg aagtagtccg
     1381 tcgatttctg cggcattgtc tcgaagtgtt ttgttacatc ctcaacctgg gttgtgagtg
     1441 tcggattcat gaggaagacg cactggtgag tgcccttggc cacaggttcg accgcttccg
     1501 gaacatacct ttcatagtca acgtttgtct gggcagcaag ctttgcatcg tcaatcccca
     1561 gctgtttttc aagtatcagc ttgtggagga ttgttacatc cagcctcttc cagtcattgg
     1621 atttttcaac cggaatggaa tcggcgattg acaggtcctt gagagtcagc atgtacatct
     1681 tttttgaacc cttcgggaag tatccgaagg cgtgcttgtc cacgtttttg cccatcattt
     1741 caaccagttt ccttgcggca tcgactgctg atccggaaac ttcaacctct gctacatcaa
     1801 aattgccctt gagctgttca agcagttttg cgccatcaaa atttgggaca tcacggacca
     1861 gcctgtgggt tgggaaaacg taaaggtttg gatcggccat gttgacatag gcgatcatcc
     1921 tgtagttcca gccttctttg ccagtgaaac ccggattctt ttccctcatc tcgttgcgat
     1981 aattgagggc ggtttcgtac ctgtggtggc catcacagat gatggttatt tttgggtcca
     2041 tcaggtcctg cacctttttt attgttgccg ggtcgctaat tttccagatt ttgtggtggg
     2101 tcttgtattc gtccacagca tcaaagaggg gccccgagcc ggttttcccg gagagcacct
     2161 tgttgattgc gtcctcattg tctctgtaga acatcaggac gaggccagta tttgtttttg
     2221 ttgacctcat gagcatgagt ctgtcctgct tggggccaga aagtgtgtgc tcgtgcggat
     2281 agacaatctt ttccgaatac tcgtggagtt tgcctagtgc tatgaagctc atgcgggtaa
     2341 cggtcttgcc agtatcagga tggatatatt cctggttgta gacgtagatg cacggctctt
     2401 ggtcgcgctc aaaaatgttc tcggccatcc acagctttgt gtaccattct gccctgacat
     2461 acttgttgta gttctcgtcg tccccctgga ggtcctttcc cagctcaacg cggatggcgt
     2521 tgtaaggcga ggtcttatag tattcctcct gcatttttgg ggtgattttg tcgtatggct
     2581 gggtgatcag gtcctgtatg ttaccagcct gtttgctgta gtggactcct ttaaagggga
     2641 gtatttccat cgatttctat tttccttcct tgaagaattc ggcgatcttt tctgccagtt
     2701 gaacgccagc ccttgtttga ccttcttcgg tggcggctcc aatatgtgga gtaagggcaa
     2761 cattcggcag ggaagccagc ttgtttactc cttccggtgg ctccttctcg aagacatcga
     2821 taccggccgc gaagagtttt ccttctttta gcgcattgta gagcgcctct tcgttgacag
     2881 taccgcccct tgcgcaattt acgagcacaa ggcctttctt gcacttgtca aagtcctgct
     2941 tatccagaat gttttttgtt tcaggggtaa gcggaatatg gagcgagatg aaatctgctt
     3001 ttgcaagcaa ttcatctttg cttaccatgt gtatgccctg catgcctgga tcggaaacgt
     3061 atggatcaaa agcaataact tccattccaa atgccattgc cctcttagct gtttccctgc
     3121 cgattcttcc aacaccaatg agtcccagtg tttttccgta gagctccacg ccttccatga
     3181 ccttcttttc ccacttgccc tctttcatgg tggctgttcc gtagccgatc cagcgggaag
     3241 cggcaagcat gtggccaatt gccagctctg caacactgat tgatgttgct gccggggtat
     3301 ttacgacctt gatccccttt tcttttgcag cattggagtc aacattgtcc agaccaacac
     3361 cggcccttcc gatgatcctc agttttgaac cggcctttat tacgtcagca gtgacctttg
     3421 tggccgacct gacgatgatg gcatcatagt ccccgattga actgacaagc tcctcgggcg
     3481 tcattttttt gacaacgacc tcatgtcctt ctttttgcag aaggtccact cctgcttttg
     3541 ctattggatc agaaatcaat attttggcca taaaaacacc cccttttgga tttattcctg
     3601 tgccatctcc ttcataaagg cagagacgcc agaaccgatt tcaaatttgt gtccaagctc
     3661 tttgagtgta agctccaggg ctgcgaggca ggctagctcg gagtacatgt ccatatatcc
     3721 catgtgacca agcctgaaga ttttgcctga gaagtcgccc tgaccaccgg cgattgttat
     3781 gccgtacttg tcccttatga ctgaggttac ctttttgccg tcaacgccat ccgggatctt
     3841 gactgttgtt actgcatcgc ccgggcattt tgcaaacagc tcgaggttca ttgctttcat
     3901 ggcagttctg atgcctttgg cgagctttgt tgatctggcc cagacgtttt caagcccttc
     3961 ggccttcatc atctcgaaag ctttgtcggc ttggtaaatg atcccgattg gtggtgtcca
     4021 cggggtttcg ttgtcaacaa gtttcttctg gaacagcttg aggtcaaaat agaacttgtg
     4081 ctgtttgttg ttttcaacga gcttccatgc tttttcgttc atgcagatga aagccattcc
     4141 tggtggaagc atcatgcctt tctgggaacc accgactacc acatcaacgt tccactcgtc
     4201 catcttgagt ggcacaacgc cgatgcctga aacgccatca accacgagta ttgcgttggt
     4261 tttgctgact atttttgcga aacctgcagt gtcagagcat gcgccggtcg atgtttcgga
     4321 gagctggcag aaaacagcaa ctgtatccgg attttcttta atcactgctt caagctctgc
     4381 tggggttgcc atgtcgcccc agtctttttt aatttcgata acatttgcac cgtaggcctt
     4441 attgatcttc acccatcttc caccaaagtt tccggcattg attacaatga ctttgtctcc
     4501 agtgttgaca aggttctgga cggctgtttc catggcgcct gatcccgatg aggccaggag
     4561 ataggagtca tacttggtcc caaggagcca tttcatgtcg tccgtcacct tgcggaacat
     4621 cttcttggcg actggatgct tgtggtgcgt tatttccctt gcgccctcaa gccttacctc
     4681 ttctggaagc ggggttggac ccggtgcgag caaaaatttg tacaatgttt tcatcaaaaa
     4741 actcctttcg gtttattgtg gccggggcgg tgttccgccg cggttaactg catggttttg
     4801 cgtcaaaagc tctatgttat tttgtgtccg ggtttttttg atatgaggat acttacgagg
     4861 cgattctttt ctgtttgaag cgctgttccc atgcaatcgc ggttgcattt tg
//
LOCUS       DAOSRI010000128         4895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15692_length_4895_cov_4.216942, whole genome shotgun sequence.
ACCESSION   DAOSRI010000128 DAOSRI010000000
VERSION     DAOSRI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15692_length_4895_cov_4.216942"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PK717_04770"
     CDS             <1..1455
                     /locus_tag="PK717_04770"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47007.1"
                     /translation="RNGSVDYHDARGLDVNAQTSFYLWAEDLFYVGGLVGDNVYCNTQ
                     VKADLVGYPPYSDKTNPRYMVKRFRHGVTNDTVFFMDWEAIPDKVAQVSYPRIELFYA
                     ENGNEVSKDLLNVDNYDMVYNYDNHFIARVYPAFKEDLPLKEDGRVYVSGNQAQQWIY
                     GQTKKSEVDDMATETTFHYTPTGTGQSTTFISYLAKNKWYFADPYQLDNPEWYSLKKI
                     YHLDVGKGLEVMIASDNLAPNATSELTIYVKELGTEAVVPEATVYVEGPGIQQTSLTT
                     NAKGEVKVKVTPKSQGKIVVTAERDEYIAGSAWVMIGKDTFPPILDVDPIAPYGNKTS
                     IDVTGKTEPGVKLTVNGTSARVDDSGKFTVKITLKEGVNPIVVEAIDAAGNRSMRILS
                     TIVDITPPAFIINGEVDGKILVAKDQTTIKLSGRIDPGSKVTVNGAPAKVVYDIWEVE
                     ITMDANLPELPLSFQFIDVAENQTTHEVKLVRQP"
     gene            complement(1574..1780)
                     /locus_tag="PK717_04775"
     CDS             complement(1574..1780)
                     /locus_tag="PK717_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOR47008.1"
                     /translation="MEITLDTKIEKILETYPSSSSFFAKRGLVCYICGEAFWGTPDEL
                     CQEKGADPKTILAELVSFLKQNQR"
     gene            1871..2932
                     /locus_tag="PK717_04780"
     CDS             1871..2932
                     /locus_tag="PK717_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47009.1"
                     /translation="MRLMIDRLIFSLFGGALGGLTYTWVLRQYVVGEQTGFIVMDVVW
                     AFVACILGGGLFYLVWPEIVAFFRMLSKLFQKIMKRLAITDLMILIFALFVTVVVYIF
                     LEPIYRFALEPKVYILVTFLTTPILFIIIWMLGIGKRESIIESTRQLFGSSISQPCSN
                     QHILDSSCLIDGRIAELMTTTTLNGRFAVPSFVLFDLQRISDSQDPILSHRGKRGMMT
                     LEKLQEIMGPKLEVLHTSNRGRQIDLLLKMSRDGYLIISCDMELTLDLRRQGATVVNL
                     NEAAPALKTMIVPGEETMIAVIKEGKEQGQGVGFLDDGTMVVIEDGKKVIGQTVRIIC
                     TSLLQNPQGRIVFGKIITR"
     gene            2929..3639
                     /locus_tag="PK717_04785"
     CDS             2929..3639
                     /locus_tag="PK717_04785"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOR47010.1"
                     /translation="MKISCIIAAGGSSTRFGLDKLEVDLGGKPVLAWSVAAMANHPGV
                     SEVVVACSDPEKSKKLVYGHVPSNVKFVMGGDCREESVKKAFEIIGNCDVVLVHDAAR
                     PFINQEMIDKVIGRAKEAACVIPVIPLVGALKTIKGEFLEKSIGGAYHIAQTPQLVWK
                     KPLEFVFDRYKNRLAEFPDESAMISEYGLTVATVHGSAFNLKITSPEDIKIANCYLSI
                     FKSPRVQKVENRRRLPDR"
     gene            complement(3584..4039)
                     /locus_tag="PK717_04790"
     CDS             complement(3584..4039)
                     /locus_tag="PK717_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HOR47011.1"
                     /translation="MVDKERIESIKQLIVLAKELGVAQLSYEDEDINVAFELEGCVHE
                     HFEERFEPISIPQQKMVEEKKPVSSGGNTFAIESPLSGTYYASPAPGADPFITLGQIV
                     NPGDTLCIVEAMKVMNEIVTDKKGRVVDMYYTSGQEVDAGSPLFVLEEI"
     gene            complement(4039..4512)
                     /gene="ispF"
                     /locus_tag="PK717_04795"
     CDS             complement(4039..4512)
                     /gene="ispF"
                     /locus_tag="PK717_04795"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOR47012.1"
                     /translation="MRIGLGFDVHNTKSGKPLVIGGVIVPCEFGLDGDSDADVLCHSV
                     IDAVLGALNLGDIGSYFGVGTPDMMGVESLSLLARLTPLIGENRMTVSNLDCTIIAQY
                     PKLSDKREQMRANIARVLQTPQNRISLKFTTPKGLGPLGNKEGIACLSAILLEEK"
     gene            4614..>4895
                     /locus_tag="PK717_04800"
     CDS             4614..>4895
                     /locus_tag="PK717_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47013.1"
                     /translation="MQDIDDVLEDVESHRMSPEEGAKRVRELKMRKRWSGGLFFGVYL
                     VLQGLAVLFYQLGYFPGTGSWWNIVWPVILFTGGLKMIFSKGRRGGFSLM"
BASE COUNT         1363 a         1068 c         1165 g         1299 t
ORIGIN      
        1 cgcaatggct cggttgatta ccatgatgca cgtggtcttg atgttaatgc ccaaacttca
       61 ttctacctct gggccgaaga cctgttctat gttggcggac ttgttggaga caacgtctat
      121 tgcaacaccc aagtcaaagc agaccttgta ggctacccac catattctga caagacaaac
      181 ccaaggtaca tggtcaagag atttaggcat ggagtaacca acgacacagt gttcttcatg
      241 gattgggaag caattccgga caaggttgcc caggtttcat acccgagaat cgaactcttc
      301 tacgcagaga acggcaacga agtctccaag gatctcctca acgtcgacaa ttacgacatg
      361 gtttacaact acgacaacca cttcattgca agggtctacc cggcattcaa ggaagacctc
      421 ccgctcaagg aagatggaag ggtctatgtc tccggcaacc aggcacagca gtggatatat
      481 ggacagacca agaagtcaga agttgatgac atggcaaccg aaaccacgtt ccactacact
      541 ccaaccggaa ccggtcagtc aacaaccttc atctcctatc tggcaaagaa caagtggtac
      601 tttgcagatc cataccagct tgacaatcca gagtggtatt cactcaagaa gatctaccat
      661 cttgatgttg gcaagggact tgaagtcatg atcgcttccg acaaccttgc cccgaacgcc
      721 acctcagaac tcacaatcta cgtcaaggaa cttggcaccg aagctgttgt tccagaagca
      781 accgtttatg tcgaaggccc aggaatacag cagacctcgc tcaccacaaa cgcaaagggc
      841 gaagtcaagg taaaagttac tccaaagtca cagggcaaga ttgttgtcac tgcagagcgc
      901 gatgaataca ttgctggttc cgcatgggtc atgattggaa aagacacctt cccgccaata
      961 ttggatgttg atccgatcgc accatatgga aacaagactt ccattgatgt aaccggaaag
     1021 acagagccag gcgtcaagct gactgtcaat ggaacttccg ccagggttga tgactccggc
     1081 aagttcacag tcaagataac actcaaggaa ggcgtaaacc caatcgtagt cgaggcaatt
     1141 gatgctgctg gaaacagaag catgaggata ctctcaacaa tagtcgacat tacaccgcca
     1201 gccttcatca tcaatggcga ggtcgatgga aagattcttg ttgcgaaaga tcagacaacg
     1261 attaagctct caggaagaat tgatccaggc tccaaggtca cggtcaatgg cgcaccggca
     1321 aaggtcgtct atgacatctg ggaagtcgaa ataacaatgg atgcaaacct tccagaactt
     1381 ccactctcct tccagttcat tgatgttgcc gaaaaccaga caacacacga agtaaaactt
     1441 gtaaggcagc cgtaagaagc actaaaccgt atacaaaaaa ggccaccctt ttgggtggcc
     1501 ttttttattt ttattggaga gaggaaccaa atagagggat tttagaatta attttttgcg
     1561 gtgaatgcca tgtttatcgt tgattttgtt tgagaaaact caccagctcg gcgagtatgg
     1621 tttttgggtc tgcacctttt tcctggcaca gctcatccgg tgttccccag aaggcttctc
     1681 cacatatata acacacaagg ccccttttgg caaaaaaact tgaagatgaa gggtaagtct
     1741 cgaggatttt ttctattttt gtgtcaagtg ttatttccat gcatgaatta tggtttacct
     1801 ccatattttg tgcaagtgtt gacctcgctt gtttgtaaac atacaatcct tttataaaga
     1861 ggtgaaagcc atgagattaa tgattgacag attgattttt tctctttttg gtggcgcact
     1921 tggcggattg acctatacat gggtgcttcg tcagtatgtt gtgggagaac aaactgggtt
     1981 catagtgatg gatgttgttt gggcttttgt cgcatgcatt cttggaggtg ggctcttcta
     2041 tcttgtctgg ccagaaatag ttgctttttt caggatgctc tcaaaattgt tccagaagat
     2101 catgaaaaga ttggccatta cagatttgat gatcctcatc ttcgcgcttt ttgtgacagt
     2161 tgtggtttat atatttctgg agccaatcta caggtttgcc cttgagccaa aagtatatat
     2221 ccttgtcaca ttcctaacga caccaatact cttcataatt atctggatgc tgggtatcgg
     2281 gaagagggaa tccataattg aatccacaag gcaactcttt ggctcttcca tatcccaacc
     2341 ctgttcaaac cagcatattc tggattcatc atgccttatc gatggcagaa tagcggaact
     2401 gatgactaca acaacactga acgggcgttt tgctgtgccg agttttgtgc tgtttgatct
     2461 gcaaaggatc tctgattcac aggacccgat actttcgcac aggggcaaaa ggggaatgat
     2521 gacccttgaa aaattgcagg aaattatggg gccaaagcta gaggtgttgc atacctcaaa
     2581 cagaggcaga cagattgacc tcttgctcaa gatgtcgcgg gatggctatc tcataatcag
     2641 ctgtgacatg gaactgacgc ttgaccttag acgacagggt gcaacagttg taaatctaaa
     2701 cgaggcggca ccggccctca agacgatgat tgttcctggc gaagagacaa tgatagcggt
     2761 cataaaggaa ggaaaagagc agggtcaggg cgttggcttc ctggatgatg gaacaatggt
     2821 tgtcattgaa gacgggaaaa aagtcattgg ccagacagtg agaataattt gcacatctct
     2881 tcttcagaat ccacagggta ggattgtctt tggcaagata ataacacgat gaaaatatca
     2941 tgcatcattg cagctggtgg gtcatcaacc aggtttggct tggacaaact tgaagtggac
     3001 ctgggtggta aaccggtcct tgcctggtct gttgctgcaa tggcgaacca tcctggtgtt
     3061 tcagaggttg tagttgcgtg ttctgaccca gagaagtcaa aaaaacttgt atacggccac
     3121 gtaccctcaa acgtaaaatt tgtcatgggt ggagattgta gggaagagtc tgtaaaaaag
     3181 gcctttgaga taattgggaa ttgcgatgtg gttttggtgc atgatgcggc aaggccattt
     3241 ataaaccagg agatgataga caaggtgata ggccgggcaa aagaggctgc ttgcgtgata
     3301 cccgtgatac cactggttgg cgcactaaaa accataaaag gcgagttttt ggaaaagagc
     3361 atcggtggtg cctatcatat tgcgcaaacg ccacagcttg tctggaaaaa acccctggaa
     3421 tttgtattcg atagatacaa aaacagactc gccgagtttc ccgacgagtc tgcaatgatt
     3481 tcagaatatg ggctgactgt ggcaactgtt catggatcag ctttcaacct gaaaattacc
     3541 agcccggaag acatcaagat agcaaattgc tatctttcga tttttaaatc tcctcgagta
     3601 caaaaagtgg agaaccggcg tcgacttcct gaccgctagt gtagtacata tcaacaactc
     3661 tgcccttttt gtcggtcact atttcgttca ttaccttcat ggcttcgaca atgcaaaggg
     3721 tgtctcctgg atttacaatc tggccaaggg taatgaatgg gtccgcacca ggggcaggcg
     3781 aggcgtaata ggtgccagag agcggggatt caatggcgaa agtgtttcca ccagatgaaa
     3841 ccggcttttt ttcttcaacc attttctgct gtggaattga gattggttca aatctttcct
     3901 caaaatgctc gtggacacaa ccctcaagtt caaaggccac attgatgtct tcgtcttcat
     3961 agctgagctg tgcgacacca agctcttttg caagaacaat cagctgtttt atagattcaa
     4021 ttctctcttt atcaaccatt atttttcctc cagaagtata gctgaaaggc aggcaatgcc
     4081 ttccttgttc cccaaggggc caagaccctt cggggtggtg aatttcaatg atattctatt
     4141 ttgtggggtt tgtaaaaccc ttgcaatatt ggcgcgcatc tgttcccttt tgtcagaaag
     4201 ttttgggtat tgtgcaatta ttgtacagtc cagatttgaa acagtcatcc gattttcacc
     4261 aatcaggggt gtcagcctgg caagcaggga aaggctttcc acgcccatca tatctggtgt
     4321 gcctacacca aaatatgagc cgatgtcgcc aaggttcagt gcaccaagaa ccgcgtcgat
     4381 aaccgaatgg cacaaaacat ctgcatcact gtctccatcg aggccaaatt cgcaaggaac
     4441 aatgacccct ccgattacaa gaggttttcc cgattttgtg ttgtggacat caaatccaag
     4501 cccgatcctc atacagtctt tgactcgcaa tgtctacttt acgttacctt gccatcaggt
     4561 gtaaactctt ttcagggtgg agtgtctatc caaagtgaag gaggcagtga aaaatgcaag
     4621 atatcgatga tgtccttgag gatgttgaga gccatagaat gtcgccggag gagggtgcaa
     4681 aaagggtaag ggagttaaaa atgagaaaac gttggtcagg tggattattt tttggcgtat
     4741 accttgtttt gcaaggtctt gcagtgcttt tttaccagct tggttatttt ccggggacag
     4801 gctcatggtg gaacattgta tggccagtga tcctttttac tggcggacta aaaatgattt
     4861 tttcaaaagg aagacgtggt ggattttcgc ttatg
//
LOCUS       DAOSRI010000129         4891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15711_length_4891_cov_4.146402, whole genome shotgun sequence.
ACCESSION   DAOSRI010000129 DAOSRI010000000
VERSION     DAOSRI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15711_length_4891_cov_4.146402"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PK717_04805"
     CDS             <1..511
                     /locus_tag="PK717_04805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR47014.1"
                     /translation="YQTMEYHTAAERYYFPKSFFEAMERMDEKFCFILGAYFEEKLVA
                     SIIIIQHGGVAYSYLSATNRDYQELRPNNLLFFDMLLKCKERGCHSFVLGGGAGGEDG
                     TYLFKLNFSQLEKDFFIYDRLHIPDLYLKLIELKTEYEHNCSHEDFDPQSVSFFPIYR
                     AEWRPGENH"
     gene            498..1148
                     /locus_tag="PK717_04810"
     CDS             498..1148
                     /locus_tag="PK717_04810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOR47015.1"
                     /translation="MKIINPAWKRIEDLVLGVLGTIVLSPLLLFISLAIVIDDGFPVF
                     FIQKRMGQGEKPFNCVKFRTMVKNAVNIGTGIYTAKDDPRITKVGKHLRSFLDELPQI
                     FNVIQGSMSIVGPRPSMIYQAKRYNEEQKKRLMVKPGITGWAQVNGRNNISWPKKIEL
                     DCWYADPVAFATDMKIIFMTFSTIAKPNTVYSDAEPDDPISRLDGVANPDALEECK"
     gene            1145..2338
                     /locus_tag="PK717_04815"
     CDS             1145..2338
                     /locus_tag="PK717_04815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR47016.1"
                     /translation="MNRLKTLGGPGHYPGKIPLCRPPLPELANIAERLTISLASGILT
                     KGDQLSEFEAEVCQVLGVKYAVGVSSCTVGLTLALASLKWSKGLRHEDRRTVLVPSFT
                     FLATASAALWAGLDPVFVDADEETFNIDVKDAQKKLRSDTLAILCTHIFGNPTGVDEV
                     ISLSEKTSVPVIFDAAHGFGILHRGKGIGSEGIASSFSLTPTKLLTSGEGGFVTTNDE
                     NMAINLRILREYGTAPGKHDMVYPGLNGRMSELHAILGRWGLTKLESESDERNRRVRL
                     YKDKLSKIPGLSFQKIDPKDRCSYKDFAIRVNGDKFGLTRDELAEVMARENFECKKYF
                     YPAVSNHTYFANNKQDCPVATKLSEEMICPPLFGTMTDAQQEAVIGTIIEAHEKAQEI
                     KKVFK"
     gene            2335..3654
                     /locus_tag="PK717_04820"
     CDS             2335..3654
                     /locus_tag="PK717_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOR47017.1"
                     /translation="MIRVAIVGEGSEELVKQLEKSDVYKVACVLRNPDELARYAGQID
                     LVLAYSDVGHACNVHKFCDQLSVPLQLLPAGCIIKREVGDDELIKRLMETGLFPKRPP
                     STPGDGDYLKGKRVMITGAGGSIGSDLAIKVASFGPEHLVLLDNSENGVYEVDLDLSI
                     DGFANKTAIVTDIRDLSRLSSIMDAYHPQTMFHVAARKHVPLMEKFPEEAVTTNIGGT
                     MNALAMAKKHGVELFTFISTDKAADPANVMGATKRVAERIIVRHARENPGRYCIVRFE
                     NVLDSNGNVVYTFKRQLLRSGHLTVTSPQMNRYFITRDEASSFIVRASKIAQKAEVYV
                     PDAGKPVFIVDLAKAIAKILGYREGVDYKITFTKPREGEKMSEELLTEAEQKRAQKLD
                     GMFMCQPDTNDPEDFEENIKLVMEAAARVDRPSIIKLLQKLEPTYKI"
     gene            3651..4802
                     /locus_tag="PK717_04825"
     CDS             3651..4802
                     /locus_tag="PK717_04825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR47018.1"
                     /translation="MNVPLIIFSSQDFDDLPTRKHRIAKKFASQGSKVIYIEAPFTHL
                     SALKDPIYRPKVERKGQIREASPNLWVASPPPMMPFYGRFGFAQNSACRTLASFARKA
                     IEKTSFPKEFACLFYLPWMAPIIGLIKPKVALYDCVDDHAGYGGTSSKSFIERAEGEL
                     CKKCDTVFVTARSLAKRLSLFNPKTVYMPNAVDPELFRQVRPSPEIEKIPAPRVAYAG
                     ALRWWFDTGLMLEIAQRMPKANFIFIGSERNGELGEEGQKLRGLGNVHFLGKKPQEEL
                     PSLMSGASCGIIPFRQNSLIASVSPLKLYEYASMGLPTVSVPMEELEGMPEDVVKIAK
                     TPGDFILEIGKAITEGPNKEKIEDFVSKNTWEARIKTFEETLLRLWQEK"
BASE COUNT         1431 a         1238 c         1204 g         1018 t
ORIGIN      
        1 ctatcagacc atggaatacc acaccgcggc cgaaaggtac tattttccaa aaagtttttt
       61 cgaggcaatg gaacggatgg acgagaaatt ctgcttcatc ctcggtgcct attttgaaga
      121 aaaacttgtg gcctcgataa taataatcca gcatggtggc gtggcgtatt catacctgtc
      181 tgcaacaaac agggactacc aagaactcag gccaaacaat ctgctgtttt ttgatatgct
      241 gctcaaatgc aaggagcgtg ggtgccacag ctttgtgctt ggtggcggtg caggcggcga
      301 agacggcaca tatcttttca agctgaactt ttcacagctc gagaaagact ttttcatcta
      361 cgacaggctc cacatacccg atctctacct aaaactcatt gaacttaaga ctgaatatga
      421 gcataattgc tcccatgaag attttgaccc ccagtccgtg agtttcttcc cgatttacag
      481 ggctgagtgg aggccaggtg aaaatcatta atccagcttg gaaacgtata gaagacctag
      541 tcctgggagt tttggggacc attgttctct ccccattgct cctgtttatt tcattggcca
      601 tcgtcattga cgatggcttc cccgtatttt ttatccagaa aagaatgggg cagggtgaaa
      661 aacctttcaa ctgcgtcaag ttcaggacaa tggtcaagaa tgccgtaaac ataggcactg
      721 gaatatacac cgccaaggac gacccaagaa taacaaaggt cggaaaacac ctcaggagct
      781 ttctcgacga actgccacag attttcaacg tcatccaggg aagcatgagc attgttggtc
      841 caaggccatc aatgatctat caagcaaaga ggtacaacga ggaacagaaa aaacgcctca
      901 tggtcaaacc tggaataacc ggctgggcac aggtcaacgg cagaaacaac atctcctggc
      961 caaaaaaaat cgagcttgac tgctggtatg cggaccctgt ggctttcgca accgacatga
     1021 agatcatatt catgactttc tcaacaatag caaaaccaaa caccgtctat tctgatgccg
     1081 aaccagacga tccaatttcc aggcttgacg gtgttgcaaa cccagacgca ctggaggaat
     1141 gcaaatgaac agactcaaaa cactaggtgg acccggtcat tacccaggaa agataccgct
     1201 ttgccgtccg ccactcccgg aacttgcaaa catagccgaa aggctcacca tctcgcttgc
     1261 ctccggcata ctcacaaagg gcgaccagct ctccgagttc gaggcagaag tctgccaggt
     1321 acttggcgta aaatacgctg ttggtgtttc atcgtgcaca gtcgggctta ccctggcact
     1381 cgcatcgctc aagtggagca aggggctcag gcatgaagac aggcgcaccg ttcttgtccc
     1441 aagttttact ttcctcgcaa cagcctcggc cgcactgtgg gccggactcg acccggtatt
     1501 tgtggatgca gatgaagaaa ccttcaacat cgatgtaaaa gacgcacaaa aaaaactccg
     1561 ctccgatacg cttgctattc tctgcaccca catcttcgga aacccgacag gtgttgacga
     1621 ggtcatttca ctttcagaaa aaaccagcgt tccggtaata tttgatgctg cacacgggtt
     1681 tggaatcctt cacaggggca aaggcatcgg ctcggaagga atagcctctt cgttctcgct
     1741 cacaccaaca aaactcctga cctccggtga aggcgggttt gtaactacaa acgacgaaaa
     1801 catggccata aacctgagga tactcaggga atacggcacg gcacccggaa agcatgacat
     1861 ggtctatcca ggcctgaatg gcagaatgtc ggagctccac gccatacttg gcaggtgggg
     1921 gctgacaaag cttgagtcag aatcagacga gagaaaccgc agggtcaggc tctacaagga
     1981 caagctttca aagatccccg ggctgtcgtt ccagaagatt gacccaaaag acaggtgctc
     2041 ctacaaagac tttgccataa gggtaaacgg agacaagttc gggctaacaa gggacgaact
     2101 ggccgaggtc atggcaaggg aaaactttga gtgcaaaaaa tacttctatc ctgcagtttc
     2161 taaccacacc tattttgcaa acaacaaaca ggactgcccg gtggcaacaa aactctcgga
     2221 agagatgata tgtccaccac tgttcggaac aatgacggac gcccagcagg aagcagtcat
     2281 cggcacaatc atcgaggccc acgagaaggc acaggaaatc aaaaaggtgt tcaaatgata
     2341 agggtggcca tagtcggcga aggttcggaa gagctggtaa aacagcttga aaaatccgat
     2401 gtttacaagg tggcctgcgt cctgcgcaac ccggacgagc tggcaagata tgctggccag
     2461 atcgaccttg tgcttgccta ttccgatgtt ggccacgcat gtaatgtcca caagttttgc
     2521 gaccagcttt ccgttccgct gcaactcctc ccggcaggat gcatcataaa aagggaggtt
     2581 ggcgacgatg agctgataaa aaggctcatg gaaacaggtc ttttcccaaa acgcccacct
     2641 tcaaccccgg gtgatggcga ctacctcaag gggaaaagag tcatgatcac aggggctggc
     2701 ggttcaatag gttccgatct tgccataaag gtcgcatcgt ttggcccgga acaccttgtc
     2761 ctgctggaca actcggaaaa tggcgtctac gaggtcgacc tcgaccttag cattgacggg
     2821 ttcgcaaaca aaacagctat agtcacagac atcagggatc tctccagatt gtcgtcaatc
     2881 atggacgcat accatccaca gacaatgttc cacgtcgccg caaggaaaca cgtcccgctc
     2941 atggaaaagt tcccggaaga ggccgtcacg accaacattg gcggaacaat gaacgcactc
     3001 gcgatggcaa aaaagcatgg cgtggagctt ttcacattca tctccaccga caaggccgct
     3061 gaccccgcaa acgtcatggg tgcaacaaaa agagtggcag agagaataat tgtcaggcac
     3121 gccagggaaa accccggcag gtactgcatc gtaagattcg agaacgtgct ggattcaaat
     3181 ggaaacgtgg tctacacatt caagcgccag ctccttcgct ccggccatct tacagtaaca
     3241 tcaccccaga tgaacaggta tttcatcaca agggacgagg cctcaagctt catcgtcagg
     3301 gcatcaaaaa tagcccagaa ggccgaagtc tatgttcctg acgccggcaa gccggttttc
     3361 atagtcgacc tggcaaaggc aatcgcaaaa attctcggat acagggaagg cgtcgactac
     3421 aaaatcacct tcacaaagcc gagggaaggc gaaaagatga gcgaggagct cctgacagag
     3481 gccgaacaga agagggcgca aaagctggac ggcatgttca tgtgccagcc ggacacaaac
     3541 gacccggaag actttgagga gaacatcaag ctggtgatgg aagccgccgc aagagttgac
     3601 aggccctcaa tcataaagct cctgcaaaaa cttgagccaa catacaagat atgaacgtcc
     3661 cgctgataat cttttcatcc caggactttg acgatctacc aacaaggaag cacaggattg
     3721 caaaaaaatt tgcatcgcag ggatcaaaag tcatatacat agaagcacct ttcacccacc
     3781 tttcggcact caaggacccg atatacaggc caaaggttga aagaaagggc cagataaggg
     3841 aagcttcgcc aaacctctgg gtggcaagcc ctccgccgat gatgcccttt tacggcaggt
     3901 ttggctttgc acagaattca gcctgcagga cacttgccag cttcgccaga aaagcaattg
     3961 aaaaaacctc ttttccgaaa gaatttgcct gcctgttcta tctgccctgg atggcgccaa
     4021 tcatagggtt aataaagcca aaagtggccc tctacgattg cgtggacgac catgcagggt
     4081 atggtggcac atcatcaaag tccttcattg agagggccga gggcgagctc tgcaaaaaat
     4141 gcgacacggt ctttgtaaca gcgcgttctc ttgcgaaaag actctcgctc ttcaatccca
     4201 aaaccgttta catgccaaac gcagttgatc cggagctctt caggcaggtc aggccttcac
     4261 cggaaatcga gaaaataccg gccccaaggg tggcctatgc tggcgcccta aggtggtggt
     4321 ttgacactgg tttgatgctt gaaatcgccc agaggatgcc aaaagcaaat ttcatcttta
     4381 tcggttcaga aagaaacggg gaactgggcg aagaaggcca gaaactcagg ggccttggaa
     4441 acgtccattt tcttggcaaa aaaccgcagg aagaattgcc atcgctcatg tccggcgctt
     4501 catgtggtat aatacctttc aggcaaaaca gcctcattgc ctccgtttcg ccgctcaagc
     4561 tgtatgaata cgcatcaatg ggtctgccaa cagtttccgt accaatggaa gagctcgagg
     4621 ggatgccgga agacgtggtc aaaattgcaa agaccccagg tgatttcatc ctggaaatcg
     4681 gcaaggcaat caccgagggg ccaaataaag aaaaaataga agattttgtt tcaaaaaaca
     4741 cctgggaagc caggataaag acttttgagg agacactttt aaggctatgg caagaaaaat
     4801 aagggtggca caggtaattg ccggaactga tgttgccgga gcaaaaggct acacaataca
     4861 gcttgtaaat ctggctgaca agtcaaaatt t
//
LOCUS       DAOSRI010000130         4886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15735_length_4886_cov_4.198717, whole genome shotgun sequence.
ACCESSION   DAOSRI010000130 DAOSRI010000000
VERSION     DAOSRI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_4886_cov_4.198717"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1453)
                     /locus_tag="PK717_04830"
     CDS             complement(62..1453)
                     /locus_tag="PK717_04830"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOR47019.1"
                     /translation="MPSYRKYLESNDYENIVKAYRNPDPARTSDRDAELYCKALRKTG
                     KEKQATAFLEKVVDRGGCNYPRSVRLLARIYSVSGEYQKAIDLLQKTFDQRPTQYWYY
                     LSMGDVYYYHKKDLEAAFQVYVKGMDIGKEHLRRDILSIYRYLLKRISHCLFELGRFK
                     DVIWYFEEFKRLEPSNFYETDFVLLGQCYEKTGQKEKALEIWKEGTRRRKGRKCLKEI
                     ERVFPDEAKMIILKPPLPSKPGSVKIPVKTKIITEEDDAAQVIIESIKGIAQKGDIVT
                     FASAVAAITQARIYSAETIQPTRIARMLSGFVTASSKNSFATTSPLANPLSFQVAIEI
                     AGLLKILFATFCGALGKLIGKKGWFYMVAGPEVAMIDDMPASMAPYDYFVIPGPYNSD
                     KLAQIIKEKTGFEAAIIDANDMGIAWAVGASDGVDKKELEQFMADNPAGNEDDQTPII
                     IIRKAAATGQKED"
     gene            complement(1440..1994)
                     /locus_tag="PK717_04835"
     CDS             complement(1440..1994)
                     /locus_tag="PK717_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOR47020.1"
                     /translation="MKRIKILVTGPFSSGKTSLIKSVSEIPVVSTEKAVTDETRVIKN
                     QTTVALDFGRLTIDDDLVLYIFGTPGQERFSYMWDVLSVGAVGMVLIVDSADYKSIIE
                     ARRYLSFFLPKLQIPCVVAANKQDVTGSLNPEQVGQMLGIDDSVEVVSCVAKDKKSVK
                     NVLIKLFELVERTGLAQEDQDAIV"
     gene            complement(1991..2632)
                     /locus_tag="PK717_04840"
     CDS             complement(1991..2632)
                     /locus_tag="PK717_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR47021.1"
                     /translation="MELAGNIKDFSVIEICQFIWISKRTGKLQLFLEQSGRRFEASVF
                     FMNGGITAAFADNQNGKEAFFAICEAENGSFRFISDENSPETNIIVSMDQLLLEASGR
                     IKLYETLRREIPSTNIIYSLSLEFETYDLVFDSKQWQVIGLIDGEKSLGDISKDLGWP
                     EFDAIRIFYSLLQVGVIRRAAVKQRTDTVQPKPNDPKSKPSFISKIIDYLRKL"
     gene            complement(2640..>4886)
                     /locus_tag="PK717_04845"
     CDS             complement(2640..>4886)
                     /locus_tag="PK717_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOR47022.1"
                     /translation="LVFVMLPTNSAEEVARLQRETSQCKNQLLRYGPLPFIDAKSITD
                     YIKSCVIGSPLSPEELLEVLKTLRCSRSVKSSLEGSREDIPDVWVVASRIKTQQAIEQ
                     MIAKTVDEHGEVLDSASERLASIRVQMRLVHTKIQSKLGEIMRSSSYSKMIQDPIITI
                     RDDRYVIPLKSEYKNHFPCIVHDSSASGQTLYVEPLSVIPLNNDLRNLRLREREEIAE
                     ILRRISDEISMRSEDMEVIDEALSGLDFMFARAELSIRWNCTEPAMEDGHRLNIRGGR
                     HPLISEPIPIDIRIGSDFKMIILTGPNTGGKTASLKTVGLFVVMAQCGLHLPCEDGTE
                     MGILDDVLLDIGDEQSLTQNLSTFSSHLKNISLIIGDASSSKLVLLDELGAGTDPTEG
                     ASLAYAIAEHFYAVGCPTIITTHIGEMKGFAYNNINAVNASVGFDHETLMPTYKIHIG
                     TPGSSHAFEIAERTGLPDLVISRARALMRPEDKNSADIISKMAEDAKMIMEEREQVSK
                     ARMEAEELLRKREVELVEIEQNRKSMLDRELLKARKFFGEKLAEADEIVKRLAKASRQ
                     NKETDTLQKRLQEIHEEVAKAEEEQPSTTPVDVEGIKAGDIVYVPRFKSQGVVMDVYP
                     EKGKVLVQVGTARVQLPASSVELVNEPVEGEEIGKPEVRPEVQSVPIKLELFGMPIED
                     ALVKLERYLDSAYSAGMPFVYIVHGRGSGVLRKAIQDYLPHCPRVDRFNLAQPDEGGD
                     AVTIVFLK"
BASE COUNT         1152 a         1192 c         1047 g         1495 t
ORIGIN      
        1 gaaatcctta tttcgcagaa accattgctc aaagacaatg tttttccaat cagatcaaac
       61 atcagtcctc cttttgtcca gtagcggccg ctttcctgat aataattatt ggagtctggt
      121 catcctcgtt gccagcggga ttatcggcca tgaattgctc aagctctttc ttgtctacgc
      181 cgtcagaggc gccaaccgcc caggctattc ccatgtcgtt ggcatcaatg attgcggcct
      241 caaaaccagt tttttccttt attatctggg caagcttgtc gctgttgtat ggccctggta
      301 tgacaaaata atcgtagggt gccattgatg ccggcatgtc atctatcatt gcgacctcgg
      361 gtcctgccac catataaaac cagccctttt tgccaatcaa tttacccaaa gcgccgcaga
      421 acgttgcaaa aagtattttt aggagtcctg ctatttcaat tgccacctgg aaggagagcg
      481 gatttgcaag gggcgaagtt gtggcaaaag aattttttga tgaagctgtt acgaaaccag
      541 aaagcatcct cgcgatcctt gtgggttgga ttgtttccgc agagtagatc cttgcctgtg
      601 ttattgcagc cacagcagaa gcaaatgtta ctatgtcacc tttttgggca atgcctttta
      661 ttgattctat aataacttgt gctgcatcat cttcctcggt gatgattttt gtttttacgg
      721 gtatctttac cgagccaggt tttgacggaa gaggcggttt caggattatc atttttgcct
      781 catccggaaa caccctctcg atctctttta gacattttct gccttttctt ctcctggtac
      841 cctctttcca gatttccagg gctttttctt tctggccagt tttttcgtag cattggccaa
      901 gcagaacaaa gtcggtctca tagaaatttg atggttcgag tcttttgaac tcctcgaaat
      961 accagattac atccttgaaa cgaccaagct caaaaaggca atgcgaaatc ctttttaaaa
     1021 ggtacctgta aattgagagt atgtctctcc tgaggtgctc cttgccaatg tccatccctt
     1081 tcacatacac ctgaaaggcg gcctcaaggt cctttttatg gtagtaataa acatcgccca
     1141 ttgaaaggta ataccagtac tgcgttggtc tctggtcaaa ggttttttgt agcaggtcaa
     1201 tggccttctg gtattcacca gaaacactgt agattctggc cagcaatctc accgaacgcg
     1261 gatagttgca accaccacgg tcaaccacct tttcaagaaa agctgtggct tgtttttcct
     1321 ttccagtctt tctcagtgct ttgcagtaca gctctgcatc tctgtccgag gttcttgctg
     1381 ggtctgggtt tcgataggct ttgacaatat tttcataatc atttgattcg agatattttc
     1441 tatacgatgg catcctgatc ttcctgcgcc agtcctgttc tttcaaccag ctcaaataat
     1501 tttatcaaaa cattttttac agatttcttg tccttggcaa cacatgatac aacctcaaca
     1561 ctgtcatcaa ttccgagcat ctgcccgacc tgctccgggt ttagggaacc tgtgacatct
     1621 tgtttgtttg ccgcaacaac acacggtatc tggagttttg gcaaaaagaa tgaaagataa
     1681 cgccttgctt ctataattga cttgtaatct gcagaatcaa caatcagcac catacctacg
     1741 gccccgacgg aaaggacatc ccacatgtat gaaaatctct cctgccctgg tgttccaaag
     1801 atataaagca caagatcatc atcaattgtc agccttccaa aatcaagtgc gactgttgtc
     1861 tggtttttta taacccttgt ttcgtctgtt actgccttct ctgttgaaac aacaggtatt
     1921 tcagaaaccg atttaattaa acttgttttg ccggatgaga acggcccggt cacaagtatt
     1981 ttgattcttt tcatagtttc ctgagataat caataatttt tgatatgaat gatggtttgg
     2041 attttggatc gtttggtttt ggctgaaccg tgtctgttct ttgtttgacc gcagcccttc
     2101 ttatgactcc aacctgcaaa agtgaataaa aaattctgat tgcatcaaat tctggccaac
     2161 caaggtcttt gctgatgtcg cccagggatt tctcgccatc tatgagcccg atcacctgcc
     2221 actgctttga atcaaaaacg agatcatagg tttcgaactc caacgaaagt gaataaatga
     2281 tgttggtgga gggaatttca cgcctcaggg tctcgtaaag ttttattctt ccagaggcct
     2341 ccagtagtag ctggtccatt gaaacaatga tgtttgtttc aggactgttt tcatcactta
     2401 tgaacctgaa gctgccgttt tccgcctcgc atatcgcgaa aaaggcctcc ttgccgttct
     2461 ggttgtccgc aaaagcggct gttatgccgc cattcataaa aaaaactgat gcttcaaacc
     2521 ttcttccgga ctgctccaga aaaagctgga gcttgccggt tcgttttgaa atccaaataa
     2581 actggcatat ttctatgact gaaaaatctt tgatgttgcc tgcaagttcc atctacacct
     2641 cactttagaa aaacaattgt tacagcatca ccaccctcgt ctggctgggc gaggttgaac
     2701 ctgtcaacac gcgggcagtg cggcagatag tcttggatgg cctttctcag gaccccagaa
     2761 cccctgccat gaacgatata tacgaatggc ataccagcgg aataggcgct gtcaagatac
     2821 ctctcaagtt ttaccagcgc atcttctatg ggcatgccaa agagttccaa ttttatcggc
     2881 actgattgga cttccggtct tacctccggt tttccgattt cttctccttc cacgggttca
     2941 ttcaccagct ctacgctgct tgccggtaat tgcacccttg ccgttccaac ttggacaaga
     3001 accttgccct tttcaggata aacgtccatt acaacaccct gcgatttgaa ccttgggaca
     3061 taaacgatgt cccctgcttt tatgccttca acatcgacag gagttgtgct tggctgttct
     3121 tcctctgctt ttgcgacctc ttcgtggatc tcctggaggc gcttctggag agtgtctgtc
     3181 tccttgtttt gtctcgaggc tttggcaagt cttttgacta tctcgtcagc ttcggcaagc
     3241 ttttcgccaa aaaatttcct ggctttcagg agctcgcggt caagcattga tttgcggttt
     3301 tgttctatct caacaagttc tacttcgcgc tttctcagga gctcctcggc ttccatccta
     3361 gccttgctga cctgctccct ttcttccatt atcatcttgg cgtcttcggc catttttgaa
     3421 attatgtctg cgctgttttt gtcctccggc ctcatgagcg cccttgctct tgatatgacc
     3481 aaatcgggga ggcccgtcct ctcggctatt tcaaaggcat gagaagaacc tggtgtacct
     3541 atatggattt tgtaggttgg catgagggtc tcatggtcaa atccaaccga ggcatttaca
     3601 gcgtttatat tgttgtatgc aaaacccttc atctcgccga tgtgcgtcgt aataattgtt
     3661 ggacagccaa cggcataaaa atgctcggca attgcatagg caagagaggc accttctgtt
     3721 gggtcagttc cagcgccgag ttcgtccagc aggacaagct tggaggatga ggcatcccca
     3781 attatcagcg atatgttttt cagatgtgag gagaatgttg aaaggttttg agtaaggctt
     3841 tgttcatcac caatatcaag aaggacatca tcaaggatgc ccatctctgt accatcttcg
     3901 catgggaggt gaaggccaca ctgtgccatg accacaaaaa gaccgacagt cttcagcgaa
     3961 gctgtttttc ctccagtgtt tggcccggtg agtatgatca ttttgaagtc agagccaatt
     4021 cttatgtcga ttggtattgg ttcgcttatc agaggatgcc tcccacccct gatgtttagc
     4081 ctgtggccat cttccatggc cggctcggtg cagttccatc ttattgataa ttctgccctc
     4141 gcgaacatga agtccagacc ggaaagcgcc tcatcgatca cttccatgtc ttcggacctc
     4201 atggatattt catcagatat tctccgcaat atttctgcaa tttcctcgcg ctccctgagg
     4261 cgaaggtttc tgaggtcatt gtttagcggg ataacagaaa gaggctccac atagagtgtt
     4321 tgccctgatg cggaagaatc atggactatg catgggaagt ggttcttgta ttcgcttttg
     4381 agcgggatta catacctgtc gtcacggatt gtgattatcg ggtcctgtat catcttggag
     4441 tatgaagacg atctcattat ctccccgagc ttggactgta tctttgtgtg taccagcctc
     4501 atctggaccc ttatcgaagc aagcctttcc gaggcagaat caaggacttc tccgtgctca
     4561 tcaaccgtct ttgcaatcat ctgttcaatt gcctgttgtg tttttattct tgaggcaacc
     4621 acccagacat caggaatgtc ctcccttgaa ccctccagcg atgatttcac agaccttgag
     4681 cacctgaggg ttttcaatac ctcaagcaat tcctctggcg agagcggtga gccgatgaca
     4741 catgatttga tatagtcggt tatcgatttt gcgtcaatga acggcaatgg gccatatcgc
     4801 agtagttgat tcttgcactg ggatgtttcc ctctgcaatc tagcaacttc ttcagccgaa
     4861 tttgtgggaa gcatgacaaa aacaag
//
LOCUS       DAOSRI010000131         4878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15779_length_4878_cov_3.648352, whole genome shotgun sequence.
ACCESSION   DAOSRI010000131 DAOSRI010000000
VERSION     DAOSRI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15779_length_4878_cov_3.648352"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4019)
                     /locus_tag="PK717_04850"
     CDS             complement(<1..4019)
                     /locus_tag="PK717_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47023.1"
                     /translation="MKKLIALGCALVIALGFYWKASGEGCSWPCYGGNSRHNFYSDCG
                     PQGNNLGLFWKFETKGPVLCSASYDGDSIYIPSTDHRLYCLDPETGEEKWKFETESEM
                     RSQPYATGKMVYFGSQDHNMYCLNASNGDKLWSFKARGGIISSPTVYGTNCYFGCLGR
                     YLYCVGASLGELKWKFETKDGIISSPLVFLNKVYFGSKDHSLYAVDSETGKLFWSFKT
                     DGSIYSSVAAQNNKIYFGSDDGNVYCLESETGQLLAQFQTGATVRTSPALMGDYLYVG
                     SGDNIFCLDKNTMEEIWRYTEPKMQVTTCTLVSSKVYVGVGSTICCLDSETGEKLSVF
                     QAKGSMCQASIATDRVLAGSLDGNLYCLGDISPEIEIKPENINFGEMSKGEQKSVSLI
                     IKNKTEKDQTVILTINKSWLKLDNTAISLIPGEEKTITASIDWEKITDVGMQRASIKL
                     VWGSSQSIVLAKAYVLYELNAEFKGCGWGNSKANPQRTGSTGAECGPLTDSVAKIWST
                     GFSQPFGNSPCVVGDWIIAGPTWGKMISCLLVENGSVVWNTELDKYITSSPSATMDYT
                     IIGAQEMLYCMDTESGKKLWEFQTEGDILSNPMIIDDRVFFGSKDEYVYCLAVNTGKL
                     LWKSDELSPIVTSGTICMNRICFGGSDGKLYCLATESGSILWTFTAKGEIATAAYEAN
                     KLFFATREKMVYCIEVTTGKRVWIYEAKSQIQSSPSVSEGQVYFGSNDGSVTCLAQSD
                     GKKKWEFVTGNYVTASPSIYGKKVIVPSQDKSIYCLDSFTGEKLWSFKCQDSIKSEAI
                     IYKGNIIFGSYDKQVYCLSDTKETLTIEPKKVDFGSIQSYDLYGSPDPIMLEVGNTSE
                     EEMVVELSTASTWFVLSNKTLVIAPKSKYIVKIGLTADGITDQGNYWGQIKLKSGDYE
                     DQIDVRVYISGEDQDEEASGCEWICFKRNPARTGFEFDGCAPAATELVKIFSVKMRDM
                     VESSPATFDGRLFVGCMDGNLYCYDAENGKEIWNFETGGPIYSSPAIFEKRIYFGSND
                     SNVYCVDWEDGSLLWKFEGPSGFQSSPVITGDFDNKPRAYIGCNDGYMYCLDAKTGER
                     IWSYKTAGAVSSSPAVSFTKTSGKLSKLDRVIFGSMDGKIYCLDAISGAKYWTYDCKA
                     PVKSTPCIKDDKVYVGCHNGMFYCLNVATGYNMWTYKTGVVNSPSFFGGNIYCATSDS
                     LLICLEGKTGKNLWSYRASGGFMTSPVVCGGKVYLGTLDKKAICIDAGSPEKIWSAVL
                     PEEVISSPIISMGRLYIISEDGTLFCFSETTREVSVEPDNADFGLVEEGREPIMPITL
                     TNRIAKKVTVSITKEGDFFS"
     gene            4193..>4602
                     /locus_tag="PK717_04855"
     CDS             4193..>4602
                     /locus_tag="PK717_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOR47024.1"
                     /translation="MISGKEEQMPFEMPEAIVISGQINDILSKSTHKVSSVEYMNTDS
                     LERQGFTNKTSSESEKILKGHKLTKAYSKGKWIFIETDDGHYLLSTMETAGKILYFRD
                     GGKPAGKWSVGIGFENGSKLYYTIVGWGFFKIVD"
     assembly_gap    4603..4612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4613..>4878
                     /locus_tag="PK717_04860"
     CDS             <4613..>4878
                     /locus_tag="PK717_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HOR47025.1"
                     /translation="EQELATHAYPGKLGLNPLDKKEFTPEALSALLDEHKKTIKEFFT
                     GQRGVAGIGNGYLQDVLFLSGIHPKRKTSLLTKEEKTALFKACKS"
BASE COUNT         1386 a         1166 c         1015 g         1301 t
ORIGIN      
        1 gagaaaaagt caccttcttt tgtgatggaa acggtgacct tctttgcaat cctgttggtc
       61 agcgttattg gcattatggg ctctctgccc tcttccacaa gcccgaaatc agcattatca
      121 ggttctacag aaacttccct ggttgtttca gagaagcaga acaatgtccc atcctctgag
      181 atgatataaa gccttcccat ggaaattatc ggggaggaaa tgacctcctc cggaagtacc
      241 gctgaccata tcttctcagg ggaaccggca tcaatgcaaa ttgctttttt gtccagagtg
      301 ccaaggtaga ccttcccacc acaaaccacg ggtgaagtca taaaaccacc actggctcgg
      361 tacgaccaca ggtttttgcc agttttcccc tcaagacata tgaggagaga atcggatgtt
      421 gcacaataaa tattccctcc aaaaaatgaa ggcgagttca caacgccagt tttgtatgtc
      481 cacatgttgt aaccagtcgc aacgttcaaa cagtaaaaca tcccattatg gcaaccaaca
      541 tagaccttat catccttgat gcagggagtt gattttaccg gagctttgca atcatatgtc
      601 caatatttgg cgccagaaat ggcgtcaaga cagtaaatct tgccatccat agagccaaaa
      661 ataaccctgt ccagctttga aagcttgcct gatgtttttg taaaggaaac ggccggagaa
      721 gagctgacag caccagcagt cttatagctc caaatcctct cgcccgtctt ggcatcgaga
      781 cagtacatgt agccatcatt gcaaccaata tatgcccttg gtttgttgtc aaaatcaccc
      841 gttatcaccg gagatgactg gaaaccagat ggcccttcaa atttccacag caatgagcca
      901 tcttcccaat cgacacaata cacgtttgaa tcattggagc caaaataaat ccttttttca
      961 aatatcgccg gtgaggaata gatcggccct ccagtttcga aattccatat ctccttgcca
     1021 ttctctgcat catagcaata gaggttgcca tccatgcaac caacaaaaag cctgccgtca
     1081 aaggttgctg gagaagactc caccatgtcc ctcatcttta cagagaatat ttttacaagc
     1141 tctgtagctg caggggcaca tccgtcaaat tcaaaacctg ttctggccgg gttcctcttg
     1201 aaacaaatcc actcacaacc cgaagcttct tcatcctggt cttctcctga gatgtagacc
     1261 ctgacatcaa tttggtcttc ataatctcca gatttcagtt ttatctgtcc ccaatagttg
     1321 ccttggtctg taatgccatc tgccgtgagg ccaattttta caatgtattt gctctttggg
     1381 gcaatgacaa gtgttttgtt tgatagcaca aaccacgttg aggccgtgga gagttccaca
     1441 accatctctt cctcggaggt gttgccaacc tcaagcatga tggggtctgg ggaaccatag
     1501 aggtcataag attggatcga gccaaaatca actttctttg gctcaattgt taaagtttcc
     1561 ttggtatcag aaaggcaata aacctgctta tcataagaac caaagatgat attgccttta
     1621 taaatgatag cttcagattt tatcgaatcc tgacatttga aggaccagag tttttcgcct
     1681 gtaaatgaat ctaggcaata gatggatttg tcttgcgaag gaacaatgac ctttttccca
     1741 tagattgagg gtgaagcagt gacataattc ccagtgacaa actcccattt cttcttgcca
     1801 tctgactggg caaggcaggt cacggagcca tcattggagc caaaataaac ttggccttca
     1861 gagaccgacg gagaggattg tatctggctc tttgcttcat aaatccatac tcttttgcca
     1921 gttgttacct ctatgcaata aaccatcttt tctcttgttg caaaaaagag cttgttggct
     1981 tcatatgctg cggttgcaat ctctcctttg gctgtgaatg tccagagaat gctgcctgat
     2041 tccgtcgcaa ggcagtagag tttcccgtcg cttccaccaa agcatatcct gttcatgcaa
     2101 atggtcccag aagttacaat cggcgaaagt tcgtctgatt tccacaggag cttgcctgta
     2161 ttgacagcaa ggcaataaac atactcatct ttcgaaccaa aaaacaccct gtcatcaatt
     2221 atcatgggat tggagaggat gtctccttca gtctgaaact cccacaattt ttttccagac
     2281 tcggtgtcca tgcaataaag catctcctgg gcaccaatta ttgtataatc cattgttgca
     2341 gacggtgatg aagtaatgta tttgtccagt tcagtgttcc acactacaga gccattttcg
     2401 accagtaaac aggaaatcat ctttccccat gttgggccag ctattatcca atcacccaca
     2461 acacaaggag aattcccaaa aggctgacta aaacctgttg accagatttt tgccacagag
     2521 tcagtcaagg ggccgcactc tgcgcctgta gatccagtcc tttgcggatt tgccttgctg
     2581 ttcccccagc cgcagccttt gaattcggca ttcaactcat aaagaacata agctttcgca
     2641 agcactatac tctggcttga accccagaca agctttatcg atgccctttg cattccaaca
     2701 tctgtgattt tctcccagtc aatggaggcg gtgattgttt tttcttcacc aggaattagc
     2761 gaaatagcgg tattatcaag cttgagccat gatttattga tggttagtat aaccgtctgg
     2821 tctttctctg ttttattttt tatgatcagt gaaactgatt tttgctcacc tttggacatc
     2881 tcgccaaagt ttatgttttc tggttttatt tcaatttccg ggctgatatc acccaaacag
     2941 tagaggttac catccagaga acctgcaagt actctgtctg tggcaatgct tgcttggcac
     3001 atggaacctt ttgcctggaa gactgagagt ttttcaccag tctctgagtc aaggcagcag
     3061 attgtactgc ccacaccgac ataaaccttg gaactaacaa gagtgcatgt tgtgacttgc
     3121 attttaggct cggtataacg ccagatctct tccattgtat ttttgtccag gcaaaagata
     3181 ttgtcgccag aaccaacgta aaggtagtcg cccatgagcg ccggagaggt cctgacggtg
     3241 gcgccggttt gaaactgtgc aaggagctgg ccagtttcag attctaggca ataaacgttg
     3301 ccatcatcac tgccaaaata tatcttgttg ttttgggcgg caactgaaga atagatgctg
     3361 ccatcagtct tgaatgacca gaacagcttg ccggtctcgg aatctacggc ataaagggaa
     3421 tggtcttttg acccaaaata aaccttgttt aaaaaaacca gtggacttga aataatgcca
     3481 tcctttgtct caaacttcca tttgagctcg ccaaggcttg caccgacaca ataaagatac
     3541 ctgccaaggc agccaaaata acaatttgtc ccatatactg tcgggcttga aataataccg
     3601 ccccttgcct tgaaactcca gagtttgtct ccatttgatg cgttcaaaca gtacatattg
     3661 tgatcttgcg agccaaaata aaccatcttg ccggtggcat aaggctggga tctcatctcc
     3721 gactcggttt caaatttcca cttttcctca cctgtttcag gatcaaggca atacagcctg
     3781 tgatctgttg atggaatgta tatactatcg ccatcatagg aggcagaaca aaggactggc
     3841 ccttttgtct cgaacttcca gaagagaccc agattgtttc cctgtgggcc gcaatcagaa
     3901 tagaagttat gcctgctgtt gccaccatag cacggccagc tgcatccttc acctgaagct
     3961 ttccagtaaa aacccagcgc aatgacaaga gcacaaccca gagcaatcag cttcttcacc
     4021 aaaacctccc aggcagcaaa tgatagaaaa agtttgtatc taaaaatcca tacgttccaa
     4081 aaagcgtttc ggttcaattg gtatgatatt tgactggaag ataaattaga aggtgcatca
     4141 ctgcacaaaa acaatgaaaa ctcgggtggt tgcccgaaag agtattcgga ttatgatttc
     4201 tggcaaggag gaacaaatgc catttgaaat gccggaagca attgtcatct cgggacaaat
     4261 aaacgatatc ttatcaaaga gcacccataa agtcagttct gtagagtata tgaacactga
     4321 ctcgctggag aggcaggggt tcacaaataa aacgagctcc gaaagcgaaa aaatacttaa
     4381 agggcacaaa ctcacaaaag cctatagcaa gggaaagtgg atattcatcg aaactgacga
     4441 cggccattac ctgctctcca ccatggaaac tgcaggcaaa attctctatt tcagggatgg
     4501 cggcaaaccg gccggcaaat ggagtgtcgg gatcggtttt gaaaatggct caaaactata
     4561 ttacacaatc gttggctggg gtttcttcaa aatagttgac gannnnnnnn nngagcaaga
     4621 gctggcaaca cacgcctacc ccggaaaact ggggctcaat ccgctggaca aaaaagaatt
     4681 cacgcccgag gccctatctg ctctcctgga tgagcacaaa aaaaccataa aagaattttt
     4741 cacgggtcag cgcggtgtcg caggtattgg aaatggctat cttcaagatg tgctcttcct
     4801 gtcagggata cacccgaaga gaaaaaccag cctgttgacg aaggaagaaa aaacagccct
     4861 tttcaaggca tgcaaatc
//
LOCUS       DAOSRI010000132         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15807_length_4874_cov_3.448848, whole genome shotgun sequence.
ACCESSION   DAOSRI010000132 DAOSRI010000000
VERSION     DAOSRI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_4874_cov_3.448848"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PK717_04865"
     CDS             complement(<1..1348)
                     /locus_tag="PK717_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR47026.1"
                     /translation="MKILKNIVVGLIIFSMFLFCGPSFAAQDYGYAKIDKALIGKTGT
                     VNVIIITGDKTVVDYVGNLSYSNKFFQGTIESCINNSVESVYLRCKSISPDLTKRWVF
                     NTVINGFSCEVDASKLIDLAKLPSVKKIVDATFRYEMQINKHLPYLGVTKLRQIGTPD
                     GKKATGAGIRVGVIDSGINYKHLALGDGWGKRVKFGYDFADKDSDPLDFMGHGSHCAG
                     IIGANGKYLDTPITGVAPEATFGAYKVFTVDEGGARPDNVAAALEMAAKDKCAVANMS
                     LGRALYYGGENEVEDQAIKNATKAGMLVVVAAGNDGYRSDRLPLPIGVPAVTEEALCV
                     AATDERGKCLIEVMTKTGETKRMLANNHIWGLPSINPDDSLYEVVDGGTDPNKIPDCA
                     GKVLLIQYKDNIRSWHRLETQNFIRKLKTLKCKAFIFWSDGFDGTPFTLSYFESFED"
     gene            complement(1406..4489)
                     /locus_tag="PK717_04870"
     CDS             complement(1406..4489)
                     /locus_tag="PK717_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47027.1"
                     /translation="MKKALALIVAVAVLAGLPVIMARNTVASDVKITVEQVYGVMPDS
                     YIGSFNLSGPNTVESDYMVGYYYPMCAMKVTSDGNLIVADTAYGRVHVLGSDLSHKSV
                     FGSLGFGDGKFQYPVDCAEDGDGNLYFVDMYNCQVSKWDKYGKFIGAFGSEGQENGQF
                     IAPGGIAVDSDGNIYVSDGNTARIQKFDSSFKFKNLMNVGSETVALDSPGPIRIGPND
                     EVFVAEMKSGTIYQFKPSGDFVKAVVKSTDEKNPIFKKLGAFEVGSDGTFYLLDRADG
                     SNAIKIFDKDGKEKSRFQSVASNEEYLDGMAVGADSVYVHVYGSINPGSGVSIDNPFI
                     VPSSQRLIKFDKSGSKKGEESYNPMQEGRGGNITSCAVTSDGTLYAVSNINVDQGGKY
                     ASNILVWDAKGNYQNSISTDDLNLSNQALFSDVTVDSYDNVYVSVNEGFRGEVPGYII
                     KIATGSEMLSTVTPNSRTAISYSSEQIGKGDLGTPAGLCVDRMDNLYVADRARQSVII
                     FTSKGRKRDELGVNTSPSNVSVDPFRNMACATDGQIVVIDKKGRDIASMGGQGRKPGQ
                     LYYPHGVLFANNGAVIVSDGENGRIQIFERKEGSDFTATYTSPRMFYVPQGMCWGKDQ
                     KVYLTDNFHNVIYRLNINGYTPPGIGETGPEPPLPPQPPAPQNIPSDGEIYFQPSDVK
                     IPSGGSSDINISIRYASNVYGVGMTLKYDPQYLQLDKCEQGNFLSSDGNKNLFIYKDE
                     PKGTVKIGGPTRTSAVAGMNGEGILIKFRFVGVKEGDTKLEVSEVLVKDPDLNNIQVS
                     VTNAKVKVLPKDSTPPPVNVNIPKCSWDASFTGSGTTEKDAKLVLKTVDGSQDIKVGA
                     DGSFKFTVNLKKGANNFTITATDPAGNSKDYTFTINQATKNVVVMWVGKLVYMVNGSN
                     MNLNAAPQLIGGSTYVPMRDLGNALSCVVDWDAKEKKATYTYTDPCSGTQTVLEAWID
                     KTMGRLNGSPLDFGKAPKIIGGRTLVPLRAISSGLGADVKYEASNKQITITHPKP"
     gene            complement(4506..>4874)
                     /locus_tag="PK717_04875"
     CDS             complement(4506..>4874)
                     /locus_tag="PK717_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47028.1"
                     /translation="ISKRANQTGISGHGIILKFLAIFPNPSAPTIKFDDLSPYDPRGR
                     MIPHMGFPLELEVTDVEPLLGDLNFDGIVDMEDYFIFAQSYGAKRDELHYNPLADFNS
                     DGIIDFEDLKVLLNNLGKSE"
BASE COUNT         1348 a         1126 c          942 g         1458 t
ORIGIN      
        1 catcttcaaa gctttcaaaa taggagaggg taaatggtgt tccatcaaaa ccatcggacc
       61 agaaaataaa agctttgcat ttgagtgttt ttagttttcg aatgaaattt tgagtttcca
      121 atctgtgcca gcttctgata ttgtctttat attgaatcaa taaaaccttg ccagcacaat
      181 caggaatttt gttgggatca gtgccaccat caacaacctc gtaaagggaa tcatccgggt
      241 ttatggatgg aaggccccat atatgattgt ttgccaacat cctctttgtt tcaccagttt
      301 ttgtcattac ctctatgaga catttgccac gctcatcagt tgccgcaaca caaagtgcct
      361 cctctgttac tgctggaaca cctattggca atggcaacct atccgagcga taaccgtcat
      421 tacctgctgc aactacaaca agcattccgg cttttgtggc gttttttatt gcctgatctt
      481 caacttcatt ctcaccacca taatataacg ctcttccaag cgacatattg gcaacagcac
      541 acttatcttt tgcagccatc tcaagcgccg cggccacatt gtccggtctt gctccaccct
      601 cgtcgacagt aaaaactttg tatgcaccaa aagtagcttc cggtgcgaca ccagttattg
      661 gagtatcaag atacttgcca ttggcaccaa ttattccagc acagtgtgaa ccatgcccca
      721 taaaatcaag cgggtcagaa tctttatcgg caaaatcata cccaaatttg acccttttgc
      781 cccagccatc accaagagca aggtgcttat agtttattcc actgtcaata acaccgactc
      841 ttatgccagc gcctgtcgct tttttaccat caggtgttcc tatctgcctc agttttgtta
      901 caccaagata tggaaggtgc ttgttgattt gcatttcata tctgaatgtt gcatcaacaa
      961 tttttttcac cgaaggcagt ttggcaagat caatcaattt acttgcatcg acttcacaag
     1021 aaaaaccatt aatcaccgta ttaaaaaccc atctttttgt gagatcaggt gaaatggact
     1081 tgcaccgcag ataaacgctc tcgacggaat tgtttataca agactcaatt gttccctgga
     1141 aaaatttgtt ggagtaagaa agattaccaa cataatcgac aacagttttg tcaccagtga
     1201 ttattatgac attaacggtg cctgtttttc caattagagc tttatctatt tttgcatacc
     1261 cataatcctg ggccgcaaaa gagggaccac aaaaaagaaa catggaaaaa atgataagac
     1321 ctacaacaat gtttttgagt attttcatat cccctccatg tctacaaaaa aagggaggcc
     1381 aaaaggcctc cctttcaaaa gtttatcagg gcttcggatg agtgattgtt atctgcttgt
     1441 ttgaagcctc atactttaca tcagcaccaa gtccagacga gatagcacgt aacggcacga
     1501 gcgttcttcc accaattatc tttggtgcct taccaaaatc tagtggcgaa ccattgagtc
     1561 ttcccatggt tttgtcaatc caggcttcaa ggactgtttg tgtaccagag catggatctg
     1621 tgtatgtata tgttgctttc ttttctttag cgtcccaatc aacaacacag ctgagagcat
     1681 ttccaaggtc acgcattgga acatatgttg aaccaccgat aagctgcgga gcagcattaa
     1741 ggttcatatt ggaaccatta accatgtaaa cgagctttcc aacccacatg acaacaacat
     1801 tttttgttgc ctgattgatt gtaaaggtat agtcttttga attacccgct ggatcagttg
     1861 ctgtaattgt gaagttgttg gcaccttttt tcaaattcac tgtaaacttg aagcttccat
     1921 ctgcaccaac ttttatgtcc tggcttccat caacagtctt gagtacaagt tttgcatctt
     1981 tttcagtggt tccggaaccg gtaaatgaag catcccaaga acattttgga atattgacat
     2041 ttactggtgg tggcgtgctg tcttttggca acactttaac tttggcattt gtgactgaaa
     2101 cttgaatatt gttaagatct ggatctttaa ctaatacctc tgatacttcg agctttgtgt
     2161 cgccctcctt gacaccaacg aaacggaact ttataagtat gccttcacca ttcattccag
     2221 caaccgctga tgttcttgtc gggccaccaa tttttactgt accctttggc tcatctttgt
     2281 agataaagag gtttttgttt ccgtcagagg aaaggaaatt cccctgttcg cacttgtcaa
     2341 gctggagata ttgcgggtca tatttgagtg tcataccaac accatagaca tttgacgcat
     2401 acctgatgct gatattgatg tctgatgatc cacccgatgg aattttcaca tctgatggct
     2461 ggaagtaaat ctcaccatct gatgggatat tttgtggggc cggtggttgt ggtgggagcg
     2521 gcggttctgg accagtttcg ccaattcctg gtggtgtgta accattgatg ttgagcctgt
     2581 aaattacatt atggaagtta tctgtgagat aaaccttctg gtccttgccc cagcacattc
     2641 cctgcggaac atagaacata cgaggagaag tatatgtggc cgtgaaatca gaaccctctt
     2701 tgcgttcaaa aatctggatt cttccatttt cgccatcaga aacaattact gctccattat
     2761 ttgcgaaaag aactccatgg ggataataga gttgtcctgg ttttcttccc tgaccaccca
     2821 tactggcgat gtctctgcct tttttgtcaa tgacaactat ctgtccatcg gttgcacagg
     2881 ccatattgcg gaatgggtca accgaaacat tgctgggtga agtattgaca ccaagttcat
     2941 ccctttttct ccccttgctt gtaaatatga tcactgactg tcttgccctg tcggcaacat
     3001 aaaggttgtc catacgatca acacacagac ctgctggagt tccaagatca ccttttccga
     3061 tttgctcgga tgagtaagaa atagcggtcc ttgaatttgg ggttaccgtt gaaagcattt
     3121 cggagccagt tgcaatcttt atgatgtaac ctggaacctc gcccctaaaa ccttcattga
     3181 cagaaacata aacattatca tatgaatcaa ctgttacgtc agagaagaga gcttgatttg
     3241 aaagattaag atcatcagtt gaaatcgagt tctgatagtt tccttttgcg tcccaaacaa
     3301 ggatattact agcgtattta ccaccctgat caacattgat gtttgaaacg gcatagagtg
     3361 tcccatctga agtcacagcg catgatgtga tgtttccacc cctgccctct tgcatcgggt
     3421 tgtatgattc ctcacccttt ttggaacctg atttgtcaaa tttgatgagt ctttgtgagc
     3481 ttggcacaat aaacgggttg tcaatcgaga cgccagaccc tggattgatt gaaccataaa
     3541 catggacata aacactgtct gctccaactg ccatgccatc aagatactcc tcattggaag
     3601 caacgctctg gaaccttgac ttttctttcc catccttgtc aaaaatcttt attgcgtttg
     3661 aaccatctgc acgatcaagg agatagaatg tcccgtctga accaacttcg aaagcaccca
     3721 gctttttaaa aatgggattt ttttcgtcgg ttgatttgac aactgccttt acaaaatcac
     3781 cggatggttt gaattgataa atggtcccac ttttcatttc tgcaacaaaa acttcatcat
     3841 ttggtccaat tctgatcggt ccaggagaat caagagcaac ggtctcactt ccaacattca
     3901 ttaagttttt aaatttgaag gaagaatcaa acttttgtat tctggctgtg ttgccatcgg
     3961 aaacataaat atttccatca ctatcaactg ctattccacc tggtgcaata aactgcccat
     4021 tctcctggcc ttcagagcca aaagcgccaa taaatttgcc atatttgtcc cattttgaaa
     4081 cctggcaatt atacatgtct acaaaataaa ggttgccatc cccatcttcg gcacaatcaa
     4141 ctggatattg aaacttgcca tcgccaaaac caagggatcc aaaaacactc ttgtgggaga
     4201 gatccgagcc aagcacatgg actcttccat aggcggtatc ggcaactatc aaattgccat
     4261 cacttgttac cttcatcgca cacatcggat aatagtagcc aaccatatag tcagactcca
     4321 ctgtattcgg gcctgaaagg ttgaatgagc cgatgtaaga atcaggcatt acaccataaa
     4381 cctgctccac tgtaattttc acatcggaag cgacggtgtt tcgagccatg atgactggca
     4441 aacctgccag cacggcaact gcgacaatca atgcaagcgc ttttttcatt tagactgccc
     4501 tttcttcact cacttttgcc aagattatta agcaatactt tcaaatcttc gaagtcaata
     4561 ataccatccg agttgaaatc agcaagaggg ttgtagtgta attcatccct ctttgcacca
     4621 tacgattgcg cgaaaataaa gtagtcttcc atatctacaa ttccatcgaa attgaggtcg
     4681 ccaagtagtg gttcgacatc ggtaacttca agttccagtg ggaaacccat gtgtgggatc
     4741 atccttcctc ttggatcata tggactaaga tcatcaaatt taatggttgg cgcagacgga
     4801 ttggggaaga tggccaagaa cttcagaatg ataccatgtc cgctaatgcc agtttgattt
     4861 gctctcttgg aaat
//
LOCUS       DAOSRI010000133         4861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15855_length_4861_cov_4.220766, whole genome shotgun sequence.
ACCESSION   DAOSRI010000133 DAOSRI010000000
VERSION     DAOSRI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_4861_cov_4.220766"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="trpS"
                     /locus_tag="PK717_04880"
     CDS             <1..957
                     /gene="trpS"
                     /locus_tag="PK717_04880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOR47029.1"
                     /translation="RPTGRMHIGHYLSVLLSYVEMQHQHECFFLVADLHVLTTSADKT
                     SEIKENIFQMACDWFAAGIDPQKSAVLVQSEVPIHYNLYLVLSMLTPTGWLERNPTVK
                     DMVRDMNLQDSVGHGLLGYPVLQAADILAYKANLVPIGKDQRPHLEISREIARRFNSM
                     YGETFPEPQEFLRDYPLIPGTDCRKMSKSLDNAIFLSDGPQEIQQKISQMVTDPEKIR
                     KADPGHPDICSVYDFHKILKTPNLKDRAEGCKKGELGCVACKKELAAFMDGIIAPLRE
                     KRAGYESNPKMVWEILNEGSRKANEFTTPIWNEVKERMGLAY"
     gene            947..1642
                     /locus_tag="PK717_04885"
     CDS             947..1642
                     /locus_tag="PK717_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47030.1"
                     /translation="MPIDNVVNPNSLGLIIQSARAGKVSPYNIRLAVITEKLLEIIVQ
                     SDGVTASQLEALDKVASLLLMKSEFVANGGRFIEKTKPSVFETGEFEGRLEGNFNALC
                     QLKDLIKRNFELDAGSFPRPSPEVVCSEEISVPTSKRLVAIIARLAKKREVDQTPIIV
                     RRKTIDLRAAMGEIMDRLKKGIQLLFSLLLTKNSTRFDVITNLLAILELAKRKRVLLY
                     QEEPFGPIYIDKA"
     gene            1639..2220
                     /locus_tag="PK717_04890"
     CDS             1639..2220
                     /locus_tag="PK717_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOR47031.1"
                     /translation="MNIQNTIEAILFASNKPLAIKRIAHLAKCDQSEVNQALGYLAIR
                     YRDSGIFLKITNGHAQLLTNPEYEEFIKPLVSRATKRKMTSAAIEVLAIVASKGEASC
                     PQISKYRGKPSEMTIQGLVKKGLLERIEKPSVTKKRKVSYIVTDKFRQMAGVSGIEEM
                     KAHVTFLTTDEETGEEGLFEEVSLEETLEQATD"
     gene            complement(2210..2464)
                     /gene="minE"
                     /locus_tag="PK717_04895"
     CDS             complement(2210..2464)
                     /gene="minE"
                     /locus_tag="PK717_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOR47032.1"
                     /translation="MSFFFRAKPSASMAKERLRMVLIQDRSLLSPGMLDSMRKDMIEV
                     LNKYFVVDNEGIEIDIDSKDTSTMLVANIPIIQTKRRTNQ"
     gene            complement(2461..3264)
                     /gene="minD"
                     /locus_tag="PK717_04900"
     CDS             complement(2461..3264)
                     /gene="minD"
                     /locus_tag="PK717_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOR47033.1"
                     /translation="MGRSIVVTSGKGGVGKTTLTANLGYGLSLEGKKVVLVDTDIGLR
                     NLDVILGLENRVVYDVVNVAEGTCELQQALVKDKRLGSSLFLLPAAQTRRKEDISNDQ
                     LKQIVTALKGAFDYVILDCPAGIEHGFNNAVNSADEAVVVVTPEVSSVRDADRVIGLL
                     ESAGVNDISLVVNRIKPEMVSSGEMMSVEDVLDILAVRLLGVIPEDRQVIVSTNKGEP
                     IILNEKSLATSAIKAIVDRLEGREVPIPTFGKRGFWARLSDAITGRRKQ"
     gene            3375..>4861
                     /gene="gpmI"
                     /locus_tag="PK717_04905"
     CDS             3375..>4861
                     /gene="gpmI"
                     /locus_tag="PK717_04905"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOR47034.1"
                     /translation="MKTGNKVLLLILDGFGVAPPGPGNAITLAGTPNIDELQRKYPHT
                     LLEASGLAVGLPKGQMGNSEVGHLNLGAGRIVYQKLTLINKSIEDGMFQKNPTLLKAI
                     EDGKKGKLHLAGLCSDGGVHSVLEHLYEIVKTAKSCGVENVFVHAFLDGRDTPPQSAK
                     KYIAEIEGKLKEIGCGKIATVQGRYFSMDRDNRWDRVKIAYDCLTAGKGRHARSAIEA
                     VDQAYLLGETDEFVTPTIIMDGDKPLALIEDGDSFIHFNYRPDRARELTKALTEKDFT
                     GFERDKLPKISYTCFAMYDETFTLPIIFTEELLKQDIETTLGKIVSDAGLSQLRIAET
                     EKYAHVTYFLNGGREETYKGEDRVLVPSPKVPTYDLQPEMSCPQVEEKLVEDIKTEKH
                     SFIVCNLANPDMVGHTGNIDATIKTIKQVDESTGKIVNAAHEHSYDVVIISDHGNAEQ
                     LLDENGNKETAHSTNLVPCIFIPSEGRKPWEFGEGGSLSDVTGLIL"
BASE COUNT         1441 a         1143 c         1141 g         1136 t
ORIGIN      
        1 aggccaacag gccgcatgca catcggacac tacctttcag ttctgctttc ttatgtcgaa
       61 atgcagcacc agcacgaatg ttttttcctt gttgcagacc ttcacgtcct gacaacaagt
      121 gctgacaaaa cttcagagat aaaggaaaac attttccaga tggcctgtga ctggttcgcg
      181 gcaggaatag atccacagaa atcggctgtc ctcgtccagt cggaagtccc tatacactac
      241 aatctctacc ttgtgctttc aatgctgacc ccaacaggat ggctcgagag aaacccgaca
      301 gtaaaggaca tggtcaggga catgaacctc caggacagcg ttgggcatgg attgcttggc
      361 tatcctgtgc tccaggccgc ggacatactc gcctacaagg ccaaccttgt ccccatagga
      421 aaagaccaga ggccacacct tgagatttca agggaaattg ccagaaggtt caattcgatg
      481 tatggcgaga cattcccgga gccacaagaa ttcctgaggg actacccatt aataccaggt
      541 acagattgca ggaaaatgag caaaagtctc gacaatgcta tcttcctttc cgatggccca
      601 caagaaatac agcaaaagat ttcgcaaatg gtcactgacc cggagaagat aagaaaagct
      661 gacccgggcc acccagacat ctgctcggta tatgatttcc acaagatcct caaaacgcct
      721 aaccttaaag acagggccga aggttgcaaa aagggagagc ttggttgtgt cgcatgcaag
      781 aaagagcttg ccgctttcat ggacgggatc attgccccgc tcagggaaaa aagggccgga
      841 tacgaatcaa atccaaaaat ggtttgggaa atcctgaatg aaggctccag aaaggcaaac
      901 gaattcacaa cgccgatctg gaatgaggta aaagagagga tgggccttgc ctattgacaa
      961 tgttgtaaat ccaaacagcc ttgggctgat tatccagtcg gccagggccg ggaaagtttc
     1021 accatacaac atcaggcttg ctgtaataac tgaaaaactt ctagaaataa tcgtgcagtc
     1081 agacggcgta acggcttcac agcttgaagc gcttgacaag gttgcaagcc tgttactcat
     1141 gaaatcagag ttcgtggcaa atggtggaag gtttattgaa aaaacaaagc cttccgtgtt
     1201 tgaaaccggc gaattcgagg gcaggcttga gggtaatttt aacgcactgt gccagctcaa
     1261 agatctcata aaaagaaatt tcgagctcga tgcgggcagc tttccaaggc cttccccaga
     1321 agtcgtgtgc tcagaagaaa tatcggtccc aaccagcaag aggttggtag caataatcgc
     1381 cagattggca aaaaagcgcg aagtcgacca gaccccaatt atcgttagaa gaaaaacaat
     1441 cgatctgagg gcggcgatgg gcgaaataat ggaccgtctg aagaagggca tccagctcct
     1501 tttttcgctg ctcctgacca aaaactctac aaggtttgac gtgataacaa atctcctggc
     1561 catactcgag cttgcaaaaa gaaaacgtgt cctcctctat caggaggaac cattcgggcc
     1621 aatatacatc gacaaggcat gaacatacag aatactatcg aggccattct ttttgcctca
     1681 aacaaacctt tggcaatcaa gcgaatcgcc cacctggcaa aatgcgatca aagcgaggta
     1741 aaccaggcgc tgggctacct ggcaatcagg tacagggaca gcggcatctt cctcaaaatc
     1801 accaatggcc atgcccagtt gctcacaaac ccggagtatg aggaattcat aaaaccactt
     1861 gtttcaaggg ccactaaaag gaagatgact tctgccgcaa ttgaagtgct tgccattgtt
     1921 gcaagcaaag gagaggcctc ttgccctcaa atttcaaaat acagaggcaa accctctgaa
     1981 atgacaattc agggactagt gaaaaagggg ctccttgaaa gaatagaaaa accctctgtt
     2041 acaaagaaga gaaaggtctc atatattgtg actgacaaat tcaggcaaat ggccggggtt
     2101 tcgggaatcg aagagatgaa agcccatgtt acttttctga cgacagacga ggaaacaggt
     2161 gaagaaggcc tctttgaaga ggtctctctg gaagaaacgc tagaacaggc tactgattag
     2221 ttcttctttt ggtctggatg atcgggatgt ttgccacaag cattgtcgag gtgtccttgc
     2281 tgtctatgtc aatttcaatg ccttcattgt caacgacaaa atatttgttg agcacctcta
     2341 tcatatcttt tctcatgctg tcaagcatcc cgggagaaag cagcgacctg tcctgtatca
     2401 aaaccattct tagccgttcc ttggccatgc tggccgatgg tttggccctg aaaaaaaagc
     2461 tcattgtttc ctcctgccgg ttattgcatc agagagtctt gcccagaaac ccctcttgcc
     2521 aaaagttggg attgggacct ccctgccttc aagcctgtca acgatggctt ttatggccga
     2581 agtggccagt gatttttcgt taagaattat tggctctccc ttgtttgttg agacaattac
     2641 ctgtctgtcc tccgggatga cacccaaaag tctgacagcg agtatgtcaa gcacgtcctc
     2701 gacactcatc atctctccag aggagaccat ttctggtttg atcctgttta cgacaagtga
     2761 gatgtcattc actcctgctg attccaaaag gcctatgacc ctgtctgcgt cccttacgga
     2821 tgagacttcg ggtgttacaa caacaaccgc ctcgtccgca gaattgaccg cattgttgaa
     2881 accatgctcg atgcctgcag gacaatccag aattacatag tcaaacgcac ctttgagcgc
     2941 tgttacaatc tgcttcagct ggtcatttga tatatcttct tttcttctgg tctgggcggc
     3001 aggcagcaaa aacaggctgg agccaagcct tttgtctttg acaagggcct gctgaagttc
     3061 acaggttccc tctgcgacat ttacaacatc atagacaacc ctgttttcaa gacccagtat
     3121 gacatcaaga tttctaagac ctatgtccgt atcaaccagg acgacttttt tcccttcaag
     3181 cgacagccca tacccaaggt ttgcggttag cgttgttttt ccaacgcctc cttttcctga
     3241 agtaacaacg atagatcttc ccatattgct agaagattgt aagtatggat gtgcttttgt
     3301 caatgttttg gcaaaatcgg acgctaataa tcgtggtact tgaagcgttt tggtgttgaa
     3361 gtaaacttga ttgcgtgaaa acaggcaaca aagttttact tcttatactg gatggtttcg
     3421 gggtggcacc acctggccca ggaaatgcca ttactctggc cggaacaccc aacattgacg
     3481 agctccagag aaaatatccg cacacactcc ttgaggcatc aggactggcc gttggactcc
     3541 caaagggaca aatggggaat tcggaagtcg ggcacctcaa tcttggcgca ggaaggatcg
     3601 tttaccagaa actgacgcta atcaacaaat ccattgaaga tggaatgttc caaaaaaatc
     3661 cgacattgct gaaagcaata gaggatggca aaaaaggcaa actccatctg gctgggttat
     3721 gctctgatgg tggtgtccat tcggttctgg agcacctcta cgaaatagtc aaaacagcaa
     3781 aatcatgcgg ggttgaaaac gtatttgtgc atgcctttct tgatggaagg gacacaccac
     3841 cacaatccgc caaaaaatat atcgccgaaa ttgagggcaa actaaaggaa atcggctgtg
     3901 gaaaaatagc aaccgttcag ggaagatact tcagcatgga cagggacaac cgctgggaca
     3961 gggtaaaaat agcctacgat tgcctgacag ctggaaaagg gagacatgcc agatcagcaa
     4021 ttgaagccgt tgaccaggcc tatctgctgg gcgagacaga tgaatttgta acacctacga
     4081 tcataatgga tggtgacaaa ccactggcac tgattgaaga cggcgactca ttcatacact
     4141 tcaattacag gcctgacagg gcaagggagc tcacaaaagc tcttaccgaa aaggatttta
     4201 caggatttga aagggacaaa ttaccaaaga taagctacac atgtttcgca atgtatgatg
     4261 aaacgtttac tcttccaata atttttacag aagagctttt gaaacaggac atcgaaacca
     4321 cccttggcaa aattgtttcc gatgcaggac tctcccagct tcgcatcgca gaaactgaaa
     4381 aatacgccca tgttacatat tttctcaacg gaggtaggga ggagacttac aagggcgaag
     4441 acagggtcct tgtgccatcg ccaaaggtcc ccacttacga tttacagccg gaaatgtcat
     4501 gcccgcaggt tgaagaaaaa cttgtggaag acatcaaaac cgagaagcac agctttattg
     4561 tctgcaatct ggcaaaccct gacatggtag gtcacactgg caacattgac gcaacaatca
     4621 aaaccataaa acaggtcgat gaatcaaccg gaaagatagt caatgcggcg catgagcata
     4681 gttatgatgt ggtaatcata tcagaccacg gcaatgcaga gcagttactt gacgagaatg
     4741 gaaacaagga gactgcccac tcgacaaatc ttgtgccctg catctttatt ccgagtgagg
     4801 gcaggaaacc atgggagttt ggtgaaggcg ggagtctgtc agatgttaca gggctgattc
     4861 t
//
LOCUS       DAOSRI010000134         4831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_15987_length_4831_cov_4.108668, whole genome shotgun sequence.
ACCESSION   DAOSRI010000134 DAOSRI010000000
VERSION     DAOSRI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15987_length_4831_cov_4.108668"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="PK717_04910"
     CDS             complement(<1..2390)
                     /locus_tag="PK717_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOR47035.1"
                     /translation="MKKAIVIIVGVLFILQPLPRSTSAQESWYQKDVEIEIPPQSEVS
                     IDIKDGLPIVDGVNSKWSELRCPEWVRGDLTLAFEKLSKTSTRLSPSTFVQAATLLPD
                     KIEVLTISSSGRKPDVIRVGDFEKMPILPDSIDNGARLAIADIDGDGNSDLVVCPKNG
                     GWYYLKGPSFSVPAQSEQTGDREWLSPMTIPSTPDKVVFAKNSDTSKGMIYYDGTEIK
                     KLDQTNFTTMLPNTFATLCSKGMIYVDQFGVIRLVESKNGTPSGYSSSTDTIMPSFDD
                     EVRLAFSDNLLIAGNNDGILLSMERNGTDFTFQKKELGTKLVFGRDLSPCLADMNGDG
                     KVDLVWRDSTGIYVSDGPDFTKKTKLSVTSAGPIAVGDLNGDKKPDIVTTEKSSIKIL
                     IGPSFNPLGNQPNAECGEYVSPSIGDLDTDGKADIVLGNEKGLLCFLKGPDFKKTSLT
                     DGIDVGDYSNPCIADVDQDGKVELAIANVDGRVIVYKYNAGELQEFRSWSFIPTPPFM
                     TRADYFTKYYRESSILPWDDDKQAIETYKKLLQECPEKYLDEVAFSIANTNPEVLRLV
                     VRMGQQSVFLRNAVSIYEADKKLGYVEIVEKGDWTTCAYQTSEGRIELSKDDYYWFVV
                     HPRVYYEMPIAVNASWWEKGPDDYGITKEAWWQHQENIFEGNGVFWREGMATDTKSYG
                     DSMINTASKGKTLEEAITNLHILVNPGKKDSKNTFGYLTQDLYPWHIFRKRYGSCGEQ
                     SIVFCAIARTALIPCYVVADRGEDHQWNEVWLPQGWTHLEPSAGDTNFLDEPWGSS"
     gene            complement(2387..3535)
                     /locus_tag="PK717_04915"
     CDS             complement(2387..3535)
                     /locus_tag="PK717_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR47036.1"
                     /translation="MQKPNLQFLGLSFSIVIAAMGFQVAPSTVMGIIKELNFLNFTQT
                     ILLANAPAFMSVFVMLPIGRFLNIKNPIRWSLPAGILLASISALLLFMASYPFMLSLR
                     FLGGLATAPAYIIGSQLPLVAFQPEQRNRFSSIQTLAAPIGTILVAQTGGLVASRFGH
                     TLVYIVPLLFSLIAIIFAIKLWNAKLPETEKPTQKNSLFPSARILLMAIVWALFSGAT
                     AGAFINTSTEIGVSEGLGRSIASLGPLFLMVPSFFISPWVGSMLDKKIKRRDALIVAG
                     LIMALSILILPAGKPFWFVGSILLGLSASFIPPVVFSSPGKFEPQHNMAYALSIINLF
                     GTIGLIASTPLVGTMRDATQSWPATMIAVSVFCFAISLTGLAFTKEQK"
     gene            3661..4494
                     /locus_tag="PK717_04920"
     CDS             3661..4494
                     /locus_tag="PK717_04920"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR47037.1"
                     /translation="MALVGDIKSFKLNSLLQLITMEGKSGALKIDDGERQAKCYFQAG
                     RLYHCSIAKLTGSAAFFELLIWDEGEFMFDERGRIDLRTIYEPIDSLLIQGSRLLEEW
                     KVIKSQGFFEDEVFEVQDNIQEASSDFTMKPDDWKTLRLVDGKLNARQIAEKSGLGYL
                     STYRILFALKSAGIITSLKYKLIDLTLIPQPHRGQSLFVPKNRIASPKVGVETAMQDL
                     VYQQIDGHNTLERITARLHLDQKRISIVITQLYRQGLISLVDAKGNNIPLEKASIGDS
                     K"
     gene            4491..>4831
                     /locus_tag="PK717_04925"
     CDS             4491..>4831
                     /locus_tag="PK717_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47038.1"
                     /translation="MNQLSYTLLTAICSAATSIFAVFLTQDCLRKYLYRRNDFAIFAW
                     FLAFCDLLFGSVMFLFWAITKNHTYMVVGFVAFASFSVGIIHAMAFDNSSPSFSKRAT
                     SVVISAIPFLSG"
BASE COUNT         1250 a         1085 c         1110 g         1386 t
ORIGIN      
        1 tccgaggagc cccatggttc gtccaagaag ttggtgtcgc ctgcacttgg ctcaagatgt
       61 gtccaccctt gcgggagcca gacctcgttc cactggtggt cttcacccct gtcggcaacg
      121 acatagcatg gaatgagtgc tgtcctggca attgcgcaga acacgatcga ttgctcaccg
      181 catgagccat agcgtttcct gaatatgtgc catggataga ggtcctgcgt tagatagccg
      241 aaagtgtttt tggagtcttt tttgcctgga ttaacaagaa tgtggagatt tgttatggcc
      301 tcttcaagtg tttttccttt tgatgcggtg tttatcatag aatcgccgta tgattttgtg
      361 tctgtggcca tgccttctct ccagaaaacg ccattccctt cgaatatgtt ttcctggtgc
      421 tgccaccatg cctcttttgt gatcccgtag tcatcgggcc ctttttccca ccatgacgca
      481 ttgacggcaa ttggcatttc gtagtacacg cgcgggtgga caacaaacca gtaatagtcg
      541 tccttagaaa gctcaatcct tccttcagat gtctggtagg cacaagttgt ccagtcaccc
      601 ttttcaacga tttcgacgta tccaagcttc ttgtccgcct cgtagattga gactgcattt
      661 ctcaggaaca cactctgctg tcccattctg acaacaagcc tcaaaacttc cgggtttgtg
      721 tttgcaatgg agaacgcgac ttcatctaga tatttttcgg ggcattcctg cagcagtttc
      781 ttgtatgtct caatggcctg tttgtcatcg tcccagggca gtattgaaga ctccctgtag
      841 tattttgtga aataatctgc tcttgtcatg aagggtggcg ttgggatgaa tgaccaggac
      901 ctgaactcct ggagctcccc ggcgttgtat ttgtaaacga tcacccttcc atcgacattt
      961 gcgattgcca gctcgacctt gccatcctgg tcaacatcgg caatgcaggg attcgagtaa
     1021 tcgccaacat cgatgccatc ggtaagggaa gttttcttga aatcagggcc cttgaggaag
     1081 caaagcaggc ctttttcatt gccgaggaca atgtctgcct tgccatcggt atcaaggtcg
     1141 ccgatcgaag gtgagacata ctcgccacat tcagcatttg gctgattacc aagcggatta
     1201 aaacttggcc caatcaatat tttgatggac gatttctcgg tggtgacaat atctggtttt
     1261 ttgtcgccgt tcaggtcacc cacggcaatt ggcccggctg atgtgacaga gagtttcgtt
     1321 ttcttggtga aatctggccc atctgagacg tatatacctg tactgtctct ccagaccagg
     1381 tccacctttc cgtcaccgtt catgtcagca aggcaggggc tgaggtccct tccaaaaaca
     1441 agttttgtgc caagttcttt tttctggaag gtgaagtctg ttccgtttct ctccattgaa
     1501 agaagaattc cgtcattgtt acctgctatg agaaggttgt ctgagaaggc aagccttact
     1561 tcatcatcaa atgatggcat tattgtatcg gttgaggatg aataccctga aggtgtgcca
     1621 ttttttgact ctacaagacg aatcactcca aactggtcaa catagatcat gccttttgag
     1681 catagtgttg caaaagtatt tgggagcatc gttgtaaagt ttgtctggtc cagtttcttt
     1741 atttctgttc catcgtaata gatcatgccc ttcgaagtgt cgctgttttt tgcaaaaacc
     1801 accttgtctg gtgtagatgg gattgtcatt ggggaaagcc attccctgtc gcctgtttgc
     1861 tcggattgcg caggcacaga aaaacttgga cccttaagat agtaccagcc accgtttttg
     1921 ggacacacta caaggtcgga atttccgtca ccatcaatgt ctgcaattgc aagcctggcg
     1981 ccattatcga ttgaatctgg caggatgggc attttttcaa aatcacccac tcttatgacg
     2041 tccggttttc tgccagatga tgagatagta agcacctcga ttttgtcggg caggagtgtt
     2101 gctgcctgca caaacgtgga cggagagagc cttgttgatg tttttgaaag cttttcgaag
     2161 gcaagcgtca ggtcgcccct gacccattcc gggcacctta gttcagacca tttgctgttc
     2221 accccatcaa caatgggaag cccatctttt atgtcgattg agacttcaga ttgcggtggt
     2281 atctcgatct cgacatcttt ctggtaccag ctctcctgcg cgctggtgct cctgggcaga
     2341 ggttgcagaa taaataaaac acctacgata atcacaattg cttttttcat ttttgctcct
     2401 ttgtaaaagc caggcctgta agcgatattg caaaacaaaa aactgatact gcgatcattg
     2461 tggctggcca actctgtgtt gcatccctca ttgttccaac aaggggtgtt gatgctataa
     2521 gacctatcgt tccaaaaaga tttattattg agagtgcata agccatgttg tgctggggct
     2581 cgaactttcc tggtgaagaa aaaaccactg gtggaatgaa tgatgctgac agcccaagca
     2641 agattgagcc aacaaaccag aatggttttc ctgctggcaa aataaggatt gatagtgcca
     2701 taatcaatcc tgcaacaatt agtgcatctc ttctttttat tttcttatcc agcattgagc
     2761 ctacccatgg acttatgaaa aaagatggaa ccatcaaaaa tagcggacca agtgaggcga
     2821 ttgatcttcc aagcccctcg gaaaccccga tttcagtaga ggtgtttata aaagcacctg
     2881 ctgtggcacc agaaaaaagc gcccaaacta ttgccatcaa cagaatcctt gctgaaggga
     2941 acaaggaatt tttttgcgtg ggtttttctg tctctggaag ctttgcgttc cacaatttga
     3001 tggcaaaaat gatagcgatg agactaaaca acaatggaac aatatagaca agcgtgtgtc
     3061 caaatctgga agcaaccagg ccgccagttt gtgcgacaag tattgttcca attggtgcag
     3121 caagagtttg aatggacgaa aaccggtttc tttgttctgg ctggaaagca accagcggaa
     3181 gctgtgatcc aattatatat gctggcgctg ttgccagtcc gcccaaaaac ctgagggaga
     3241 gcatgaatgg atagctcgcc atgaacagga gtaatgctga aatggatgcg agcagaatac
     3301 ctgccggcaa tgaccatctg atgggatttt ttatattcag gaacctccca atgggcagca
     3361 ttacaaacac actcataaaa gcgggagcat ttgcaagcag tatagtttgt gtaaaattga
     3421 ggaagttaag ctcttttatg atccccataa ctgtagaggg cgcaacctga aaacccattg
     3481 cggcaatgac aatcgaaaaa gaaagtccta aaaactgcaa gtttggtttt tgcattgatc
     3541 ctcgaagtga ttgtaacaaa gtaccaatct ttgtctataa atgttttttg cccagggtca
     3601 agttgacaaa accgactcct atattaaact tgacttgaat aattcgactt tggagggcct
     3661 atggcacttg tcggagatat aaaaagcttc aaactgaact cattactcca gctcatcacc
     3721 atggagggca agtctggtgc ccttaagatt gatgatggtg aaaggcaggc caaatgctat
     3781 ttccaagctg gcaggcttta ccattgctcc attgccaaac tgactggcag tgcagcattt
     3841 tttgagcttt taatatggga tgaaggcgaa tttatgttcg atgaacgtgg gaggatagac
     3901 ctcaggacga tctatgaacc gattgattcc cttctcattc agggttcaag actccttgag
     3961 gaatggaaag taataaaatc gcaaggcttc tttgaagacg aagtttttga ggtgcaggac
     4021 aatatacagg aggcttcatc agatttcacc atgaagcctg atgactggaa aaccctcaga
     4081 ctcgttgatg gaaaactcaa tgctaggcag attgcagaaa aatctggcct tggttacctc
     4141 tcgacataca ggatactgtt tgcattgaaa tcggcaggga taatcacttc gctgaaatac
     4201 aaacttattg atttgacact gatcccacag ccgcataggg ggcaatcgct ttttgtacca
     4261 aaaaacagga tagcttcgcc aaaagttggc gtagagactg ccatgcagga ccttgtgtac
     4321 caacaaatcg atggccacaa tacacttgaa agaattaccg cgaggcttca tcttgatcag
     4381 aagcgcattt ctatcgtcat aacacaactc tacagacagg ggctgatatc gctggttgat
     4441 gccaagggaa acaatattcc gctggaaaag gcatcaatcg gtgattcaaa atgaaccagc
     4501 tctcgtatac cctcctgaca gcgatatgtt ctgcggcgac ctcaattttt gccgtctttc
     4561 ttacccagga ttgcctcaga aaatatctct atagaaggaa tgattttgcc atttttgcat
     4621 ggtttcttgc tttttgtgat ttgctgtttg gttctgttat gttcctgttt tgggcaatca
     4681 caaagaacca tacttatatg gttgtaggtt ttgtggcttt tgccagcttt agtgttggta
     4741 ttatccatgc gatggcattt gacaactcta gcccttcttt ctcaaaaaga gccacgtcag
     4801 ttgttatttc tgcaatacca tttttgtctg g
//
LOCUS       DAOSRI010000135         4752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_16307_length_4752_cov_4.176709, whole genome shotgun sequence.
ACCESSION   DAOSRI010000135 DAOSRI010000000
VERSION     DAOSRI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16307_length_4752_cov_4.176709"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="PK717_04930"
     CDS             <1..1807
                     /locus_tag="PK717_04930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47039.1"
                     /translation="GSYKYKFIENQIRTRLLEETNPKLLILIHDKCANYIQAKQKTDK
                     NESADELIEHLLGGSNPAKSIPVLLSASTMAESQFAIRQALEYAKKALEVSIRASDSS
                     LETASSLRLAKIMKLQGKFDEAEKTLAGMLSALKKAGVDTDSESEILLALADLEASQA
                     KFFEAQQRTQEAYRLVGRNDEDEKLIKLHLQESVNMKDYDFSQMALHAIKSSDSAIKS
                     PNASLRIKALLNLAYAKYFQGDFSDSLDVLEKISVEIGDTSWVRMKARFFHLKALLAI
                     VGGSMSQASEDNLMAREISFKMDSQPLLATTLCIDALIKHNQCDLDSVRRLMERAIEI
                     SILTGQKMQACRFLVKMAQFQIEDNDKDGAKFYLQRAKDIEKRVPELPIRPSIVLEAQ
                     IAFEEEQLKKCQDLMDEALNSAHCLDFEQRMALTILNIKFLEHQNEHKKALTLIKSTS
                     EKMRQVAQTPFYSCELGLLTAQAACNLLLENLQKTKKSSIISITPQWQGEMAKTAKDA
                     LDQAFLGSLSSGVRCQSFNVALAYSIYHAIMTNYDQENLKQHQAETSRYLEIAVQMCD
                     ELKKPNLSTKLKRTAEIIKMNIRSGIVKKSTFDVI"
     gene            complement(1811..2263)
                     /locus_tag="PK717_04935"
     CDS             complement(1811..2263)
                     /locus_tag="PK717_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR47040.1"
                     /translation="MTEFENEIYPMPCFATLSVTRVASSVEFYCDKLGFKKIFFMEGP
                     SGETALAHLRWAMYADLLVVPGKQEADGQKGFGISLTFACGRTSVDELAKRLTKAGLE
                     FSGPTITPWNAKELRIKDPDGFLLVFTEQADAGKDFDEVIGDIKKAQG"
     gene            complement(2260..2568)
                     /locus_tag="PK717_04940"
     CDS             complement(2260..2568)
                     /locus_tag="PK717_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOR47041.1"
                     /translation="MTVCDKEGKILYMNQKAMKTFEGDGGVSLVGQNVLDCHPEPSRS
                     KLAKMIAEGTKNVYTIEKNGIKKMIYQSPWYENGVYMGFVELSLEIPFEMPHFVRDKK
                     "
     gene            complement(2821..3249)
                     /locus_tag="PK717_04945"
     CDS             complement(2821..3249)
                     /locus_tag="PK717_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOR47042.1"
                     /translation="MEKKDGKNPSPGGRRTKDEGVLSWPDYEKRQQLLEHAARMRKSQ
                     TDAEGLLWGILRGRRFLGLKFRRQHTINGFIVDFYCNERRLAIELDGGGHNDSAKERS
                     DFLRTESLRRSGISVIRFWNNDVFNNIEAVLERLFEELKD"
     gene            complement(3390..4028)
                     /locus_tag="PK717_04950"
     CDS             complement(3390..4028)
                     /locus_tag="PK717_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOR47043.1"
                     /translation="MVLFNLTTLNSSLSTSDPNIGLFYSLVGTWKEDATLCGSETALK
                     SGTPEDDASAISSARALPVDRSKAVLAVCDSRGRVKCWTNLKLSGYWGEFVALCSKAT
                     PKEHLDYLGSVGVKVIVEGEDKVDLVKALAALERDFGAKTVRVDSGGTLNGVLLGLGL
                     VDEVSLLVQPIIAGGDGPSFAKLPGSTNIPLELISCQTLEGGYVWLRYRVLR"
     gene            complement(3994..>4752)
                     /locus_tag="PK717_04955"
     CDS             complement(3994..>4752)
                     /locus_tag="PK717_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47044.1"
                     /translation="LTSNIYIPPHLVSNMHNAEKEFGKEINDAMSEQLIMLPFLFSEA
                     IQDFFYFALENELLIRGAISEGSLFTDEDEKVYMGPAAIDAAEIYEMAEWIGIICTTS
                     TSFSILEKQKYLENRIKQKLEDVDSYYFFDDINNYIPYTVSLKQKDNSSKNIETDLMV
                     LDWPAYFFKKNQSDVIGICKIFHGNHIPIKDKTKYDNTINFFKKRTDIFKQEKERNEA
                     LDFKRDTDNLFFQLETRNLPGKINGPIQSNNAKF"
BASE COUNT         1390 a         1162 c          927 g         1273 t
ORIGIN      
        1 tggaagctac aaatataaat ttattgaaaa ccagatcagg acaaggcttc tggaagaaac
       61 aaatcccaaa ctgctcattt tgatccatga caaatgcgcc aattacatac aggcaaaaca
      121 aaaaaccgat aaaaatgaaa gcgctgatga gttgatcgaa catctactgg gtggctcaaa
      181 tccagccaaa tcaatccccg tcctcctttc ggcatccact atggcagaat cgcaatttgc
      241 aatcagacaa gcgcttgaat acgcaaaaaa agcactcgaa gtttcaatca gggcctctga
      301 ttcttccctt gaaacagcat cctcgctcag acttgcaaaa ataatgaagc tccagggtaa
      361 atttgatgaa gcagagaaaa ccctggccgg aatgctgagc gcactcaaaa aagccggggt
      421 tgacacggat tctgagtctg aaattctcct ggcacttgct gatttggaag caagccaggc
      481 aaaattcttc gaagcacaac aaagaacgca ggaagcatac aggctggtcg gaaggaatga
      541 tgaagacgaa aaactcataa aacttcacct gcaagaatcc gtcaacatga aagattatga
      601 tttcagccaa atggccttgc atgctataaa atctagtgat tcggcgatca aatcgccgaa
      661 tgccagtctt agaattaaag cattattaaa ccttgcatat gcaaaatatt tccaaggcga
      721 tttttctgat tctctggatg ttctcgaaaa aatctctgtc gaaatcggcg acacatcatg
      781 ggtgagaatg aaagcaagat tcttccatct caaagccctc ctggcgattg ttgggggaag
      841 catgtcccaa gcctcagaag acaacctgat ggcaagggag atctctttta aaatggactc
      901 gcaaccgctc ttggcaacaa ccctttgcat agatgcactc ataaaacaca accagtgtga
      961 cctggattct gtcagaagac tcatggaaag ggcaatcgaa atctcaattc tgaccgggca
     1021 aaaaatgcaa gcatgccggt tcctggtaaa aatggcccag ttccagatag aagacaatga
     1081 caaagacggc gcaaagttct atctgcaaag agcaaaggac attgaaaaaa gggtgccaga
     1141 acttccaata aggccatcaa tcgtgctcga ggcgcagatt gcttttgaag aagaacagct
     1201 aaaaaaatgc caggatttga tggatgaggc cctaaattca gctcactgtc tggattttga
     1261 gcagagaatg gcgctcacca ttttgaacat aaaatttctc gaacaccaaa acgagcacaa
     1321 gaaggctcta acactgataa aatctacctc ggaaaagatg aggcaagtcg cgcaaacacc
     1381 gttctattct tgcgaacttg gattgctgac agcgcaggcc gcttgcaacc tgcttcttga
     1441 aaacctccag aaaaccaaaa aaagcagcat aatctcaata actccacaat ggcagggcga
     1501 gatggccaaa acagcaaaag atgcgcttga tcaggccttc cttggctcgc tttcatcagg
     1561 ggtaaggtgc cagtcgttca atgtcgcgct ggcctactca atctaccatg caataatgac
     1621 caactacgac caggaaaacc tgaaacagca ccaggctgaa acatcaagat accttgagat
     1681 tgccgttcaa atgtgtgatg agttgaaaaa accaaacctc agcacaaaat tgaaaaggac
     1741 agccgagatc ataaaaatga acatcaggag cgggatagta aaaaaatcca ccttcgatgt
     1801 aatttaagat ttatccttgg gcctttttta tgtccccgat cacttcatca aaatctttgc
     1861 cggcatcggc ctgctctgta aaaaccagca agaaaccgtc tgggtccttt atcctcaatt
     1921 cttttgcatt ccatggggtg atggttggcc ctgaaaattc aagaccagcc tttgtcaatc
     1981 tcttggccaa ttcatcaaca gaggtcctgc cgcatgcaaa agtcagtgag atgccaaaac
     2041 ccttctgtcc atcagcctcc tgcttgccag gcacaaccag caaatcggcg tacattgccc
     2101 agcgaaggtg cgccagagca gtctcaccgc ttggcccctc catgaaaaat atttttttga
     2161 atcccagctt gtcgcaatag aactcgacag aagaagcgac tctggtcaca gaaagtgtcg
     2221 caaagcatgg catcgggtat atttcatttt caaattcggt cacttcttgt ccctcacaaa
     2281 atgcggcatc tcaaaaggaa tctccagcga aagctctaca aaacccatat aaacaccgtt
     2341 ttcataccac ggcgactggt agatcatctt ttttatgcca ttcttttcga ttgtgtaaac
     2401 attttttgtg ccttcagcta tcattttggc aagttttgac cgagatggct caggatggca
     2461 atctagaaca ttttggccaa caagcgagac cccaccgtca ccttcaaagg ttttcatggc
     2521 cttctggttc atatagagga ttttgccctc tttgtcacaa accgtcattg cacctggaaa
     2581 ttcatctgtc cacatgtgag caattttacg caaaaccctt cacttttcta ttaaaacccg
     2641 ccctcttttg agaaaagtca tgacttgtct aacaatcttt caggagaaaa gatttttgat
     2701 ttttttccct ttctcctttt gagagaaagg gccttgttaa tattttcccc tctcctgaca
     2761 aggggagtta ctactgtttt ttccctctcc ttccaggaga ggggtagggg tgaggtggtt
     2821 ttagtccttc aattcctcaa aaagcctctc caaaacagcc tcaatattgt taaaaacatc
     2881 attgttccaa aatcttatca cactaattcc actcctcctc aaactttccg tcctcagaaa
     2941 atcacttctt tccttcgccg aatcattgtg gccgccgcca tcaagctcaa tggccagacg
     3001 cctctcattg caataaaaat ccacaatgaa accgtttatt gtatgctgtc tcctgaattt
     3061 gagccccaaa aatcttcttc ccctgaggat cccccaaagc aaaccttctg catctgtctg
     3121 ggattttctc attctagcag catgctcaag aagctgctgc cttttttcat aatctggcca
     3181 ggataaaacc ccctcatcct ttgtccttct cccgcctgga gaaggatttt tgccatcttt
     3241 tttctccatc cagtttccct tccaaaactc ccctagcagg aggagttgct actatttttc
     3301 cctctccttt tttcaggaaa ggttgctctt ttctccctct ccttccagga gaggggtagg
     3361 ggtgaggtgg ttttaaaacc accaaaaact tacctcaaaa ccctatacct cagccagaca
     3421 tacccgccct cgagtgtctg gcaagaaata agctccagag gaatattggt cgaccctggc
     3481 agtttcgcaa acgatgggcc gtcgccaccg gcaataatcg gctggaccag caaactcacc
     3541 tcgtcaacaa ggccaagccc caaaagcacg ccgttgagcg tcccgccgct gtcgacgcgc
     3601 acggtctttg cgccaaagtc ccgctccagc gcggcaagcg ccttcacaag atcgaccttg
     3661 tcctcgccct cgacaatcac cttcacgcca accgaaccca gataatccag gtgctccttt
     3721 ggtgtggcct tcgagcagag cgcaacaaac tcgccccagt agccggaaag tttgaggttt
     3781 gtccagcatt tgaccctgcc cctgctgtcg cagacggcaa gcacggcctt tgaacggtca
     3841 accggcaagg cgcgcgcaga gctgatggcc gaagcgtcat cctcaggtgt tccagatttc
     3901 agggctgttt cgctcccgca cagcgttgcg tcctctttcc atgttcccac gagggaatag
     3961 aacaggccga tgttggggtc ggaagtcgat agactagaat ttagcgttgt tagattgaat
     4021 aggaccattt atcttccctg gcaaattcct tgtttctaat tgaaaaaata aattatcggt
     4081 gtctcttttg aaatctaaag cttcatttct ttctttttct tgtttaaaga tatctgtacg
     4141 ttttttaaaa aaattaattg tgttgtcata ttttgtctta tcttttattg ggatgtgatt
     4201 gccatgaaaa attttacata ttcctataac atcactttga tttttcttaa aaaagtatgc
     4261 aggccaatct aagaccatta gatcagtctc aatattttta gaactattat ccttctgttt
     4321 cagagaaaca gtataaggaa tataattgtt tatatcatca aaaaaataat acgaatcaac
     4381 atcttcgagt ttttgtttga ttctattttc taggtatttt tgtttttcca gaattgagaa
     4441 tgaagttgaa gttgtgcata ttattccaat ccattccgcc atttcataaa tttctgctgc
     4501 atctattgca gctggcccca tgtacacctt ttcatcctca tctgtgaaca aggaaccttc
     4561 agaaattgct cccctgataa gcaactcatt ttctaatgca aaataaaaga aatcctgaat
     4621 agcttcagaa aataaaaaag gaagcattat taattgctca ctcattgcgt cattaatttc
     4681 ttttccaaat tctttttcgg cattatgcat attagaaacc aaatgggggg ggatgtaaat
     4741 gttagaagta ag
//
LOCUS       DAOSRI010000136         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_16500_length_4708_cov_3.552977, whole genome shotgun sequence.
ACCESSION   DAOSRI010000136 DAOSRI010000000
VERSION     DAOSRI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16500_length_4708_cov_3.552977"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="PK717_04960"
     CDS             complement(<1..1911)
                     /locus_tag="PK717_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR47045.1"
                     /translation="MSQKTKKAGAIGVKAWVAGVFVYLSFAILLATFYFPGLSFTLHS
                     GNIAPKDIISPRDDQIVDKAATDAARERAAASVLTSYDFDEKVLENSIKRIDALFDLV
                     IETIRDKTLLTDAQKAEQLRFQIARSNLGFDLKPETITKLAGANTAQIEDLRSGVIDV
                     TRSLLIKGINEEGIKNITNDIETYVKLRPSSQWTRNLLLEITKFTVAQNMFVNEEQTR
                     KNRDYAKNRISPIVRTVSEGEIIVMAGEPITPDAMAIIEYLGIARNIGDWRTWLAIAL
                     YPLILVIFLYMVIAISNKIILEDTTKITLFVILVIISIAGARFLVPISPFLALTPFVA
                     IIMTIFLGAQVSIPVMLLLSPLGVIFKSIASAPVSGTMALSFGFALIGYMTCLHLPKV
                     KRFTHFVVVIVYSLIGTVLASSLFVLFSTLDKMGMLIMVAIALGSTTVQVALALALTP
                     FLELLTTHTTVFRLLELSDLNHPLLRKLMIEAPGTYQHSILVGNMASIACDEIGANGL
                     LARVGGYYHDIGKLKYPHYFIENQQGPNPHDSLSPGLSKTIITKHVSEGLELARFYKL
                     PQEIMGFVDTHHGTSLVGFFLLKAREKDPETEEDDFRYQGSHPRTIEQAVVMLADTIE
                     SASRACQMQNSFC"
     gene            complement(1963..2139)
                     /gene="rpsU"
                     /locus_tag="PK717_04965"
     CDS             complement(1963..2139)
                     /gene="rpsU"
                     /locus_tag="PK717_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR47046.1"
                     /translation="MQELKRKDNETFDSMLHRFQQVCLKDGIFAEIRKREYFMPPSIK
                     RKKKRAKAKKGKRF"
     gene            complement(2167..2499)
                     /locus_tag="PK717_04970"
     CDS             complement(2167..2499)
                     /locus_tag="PK717_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOR47047.1"
                     /translation="MSDCIFCKIIAGEIPSKKVYEDDDVICFHDIHPAASVHVLVVPK
                     RHFSSLNELDDPELAGKLFLVVPTIAKMLGVDGAYRTVVNTGKGAGQSVAHIHLHILG
                     GPRIGMPE"
     gene            complement(2492..3118)
                     /locus_tag="PK717_04975"
     CDS             complement(2492..3118)
                     /locus_tag="PK717_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47048.1"
                     /translation="MTENNKRTSDFINKYGSFITALIGFAALVAVSFFLNRNEADGKL
                     TWFWGSMVFFAAWPVESAIFTFLTESFWQHLAGSLMLAFCLTLGNFLQTQTLTWSLLL
                     APACLLWPIAYAVWSFIKKVSKSNILASLVGWIVVSAIAVAAEWFLMKRFTWSLTLVI
                     CLAFWPAISLVYVREDNPKSSQTVDAESSPDSQLDAQEPEQKEEDKNE"
     gene            complement(3115..4299)
                     /locus_tag="PK717_04980"
     CDS             complement(3115..4299)
                     /locus_tag="PK717_04980"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOR47049.1"
                     /translation="MRLDLNFSVINFGCKVNQYQGELLRENLILLGLIEATSPPYDLV
                     FVNGCMVTETASREAMRTARKFSSIARVFLTGCLGKNNAKDGFEYINPDALEELSEKL
                     GLQGNMKSEIDSFLGHTRAMVLVQLGCDNFCSYCIVPYMRGEPRNRPAKDIVEETKKL
                     ASSGFREIVLCGTELGHRKDLPDLIKSISGVRGIERIRLSSINPRHLSPSLVRELLSI
                     EKVAPHLHLPLQSGSDRVLGLMNRGYNQKHFLQLVDAAREGRDEIGITTDILVGFPGE
                     NENDFEETIKVMEMAAFSRCHVFPFSKRPGTKAYDMEAVPDEIIKARAKDARAKASSL
                     ARQAISAMINRCTKVLVESDSEGFSGSYLRVRVTGKVGLGDFVPVRITGKEKEGLVGE
                     KI"
     gene            complement(4289..>4708)
                     /locus_tag="PK717_04985"
     CDS             complement(4289..>4708)
                     /locus_tag="PK717_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOR47050.1"
                     /translation="VSKFVLVYCKRTQGRYPQDRWDKKIHSLGITAKCAAELSAREIV
                     PEIIGPMDFEKAIALSEGTKLIPWELEQSTHIAKSIKGQPSEVTIAIGPEGGLEHSEI
                     ELARSNGFIPVTLGPRILRAELAPVVAISQLLSQVEA"
BASE COUNT         1274 a         1101 c         1032 g         1301 t
ORIGIN      
        1 gcaaaagctg ttttgcatct ggcatgccct tgaggcagat tcgattgtgt cagcaagcat
       61 gaccacagcc tgttcgatgg ttcgtgggtg cgaaccttga tacctgaaat cgtcttcctc
      121 tgtctccggg tctttttctc tagctttcaa aagaaagaag ccaacaagtg atgtgccatg
      181 gtgtgtatca acaaagccca ttatttcttg cggaagtttg tagaatcttg caagttcaag
      241 gccttcagaa acatgtttcg tgatgatagt ctttgaaagg cctggcgaaa ggctgtcgtg
      301 tggattgggg ccttgctggt tctcgataaa gtagtgcgga tacttcaact tacctatgtc
      361 atgatagtaa ccgccaactc tggccaaaag accattcgcc cctatttcgt cacaggcaat
      421 tgaggccatg ttgccgacaa ggatcgaatg ctggtaagta cctggggcct ctatcatcaa
      481 ctttctgagc aggggatgat tgaggtcaga gagttcgagg agtctgaaaa cggtcgtgtg
      541 tgtggtaagc aattccagga aaggagtcaa tgccagcgca agcgccacct gaacagttgt
      601 tgagccaagc gctatcgcaa ccattatgag catacccatc ttgtccaatg ttgagaagag
      661 cacaaaaagg gaggaggcaa gtacagttcc tatcaaggag tatactatta caacaacaaa
      721 gtgggtaaaa cgttttacct ttgggagatg gaggcaagtc atgtatccga taagggcaaa
      781 accaaaggaa agtgccattg ttccagaaac gggggcagag gctatgctct tgaaaatgac
      841 cccaagaggg gaaagcagca gcatgactgg aattgaaacc tgagcaccaa gaaagattgt
      901 cattatgatg gccacaaaag gtgttaacgc caaaaatggt gatattggaa caaggaacct
      961 tgcgccagca atcgagataa taacgagaat cacaaaaagg gttatttttg ttgtgtcttc
     1021 aagaataatt ttgtttgaaa ttgcaatcac catgtagaga aaaatgacca aaataagcgg
     1081 ataaagcgca atggcgagcc atgttctcca atcaccgata tttctggcaa ttccaagata
     1141 ttcgattatt gccattgcat cgggagtgat cggttcacca gccattacga ttatttctcc
     1201 ttcgctgact gttcttacaa tagggctgat cctgtttttt gcatagtcgc ggtttttgcg
     1261 ggtttgctcc tcattgacaa acatgttttg ggcaaccgta aattttgtta tttcaagcag
     1321 gaggtttctc gtccactggc ttgagggtct gagcttgaca taggtttcaa tgtcattggt
     1381 gatgtttttt atgccctctt cgttgatgcc ctttatgaga agactcctcg ttacatcaat
     1441 gacgccactt ctcaggtctt ctatttgagc ggtatttgca cccgcaagct ttgttatggt
     1501 ttctggtttg aggtcaaaac caagatttga tctagcaatc tggaaacgaa gttgctcagc
     1561 cttttgtgca tctgtaagca aggtcttgtc ccttatcgtc tcaatgacaa gatcaaagag
     1621 cgcatcaatg cgttttattg agttctcgag gaccttttcg tcaaaatcat aggacgtcag
     1681 gacgctcgca gccgcccttt cccttgccgc atcagttgcg gctttgtcaa caatctggtc
     1741 gtccctcggg gatataatgt cctttggtgc aatattgcct gaatgaagcg tgaaagaaag
     1801 gcctggaaaa tagaaggtgg caagcaatat ggcaaaggac agataaacaa aaacgcctgc
     1861 aacccaggcc ttcacaccta tggcgccagc tttttttgtt ttttggctca tcttttttgt
     1921 aaaagggccc caaatgatac gttcttgggg cccctgttta cactaaaacc tctttccctt
     1981 cttggctttt gctctctttt ttttgcgttt gatcgaaggg ggcatgaaat actctctctt
     2041 gcgaatctcg gcaaaaatac catctttcag gcaaacctgc tggaatctgt ggagcattga
     2101 atcaaaagtt tcattgtctt tacgtttcaa ttcttgcatc tatttaatca cccccttcct
     2161 gtcggctcac tctggcatac caattctggg gccaccaagg atatggagat ggatgtgggc
     2221 aacactctgc ccggcacctt ttcccgtatt tacaacggtc ctataggcgc catccacgcc
     2281 aagcatcttc gctatcgtcg gaacgaccag aaaaagcttt cctgcaagct cgggatcatc
     2341 aagctcgttc aagcttgaaa aatgtctttt cggtacaacc agtacatgga cgctggctgc
     2401 tggatgaata tcatggaaac aaataacgtc atcatcctca tagactttct ttgatggaat
     2461 ctcgccagca atgatcttac agaagatgca gtcactcatt cttatcttcc tctttttgtt
     2521 ctggctcttg ggcatcaagc tggctgtctg gtgagctctc ggcatctact gtttgggaac
     2581 tttttggatt gtcttcacgt acataaacaa gggagattgc tggccagaaa gcaaggcaga
     2641 tgaccaaggt gagtgaccat gtaaaacgct tcattaaaaa ccattcggca gcaacagcaa
     2701 tggcagatac gacaatccag ccaaccagag aggctagaat atttgatttg gaaacttttt
     2761 tgatgaatga ccacacagca taagctattg gccacaacaa acatgctgga gcaagaagca
     2821 atgaccaggt gagtgtttgt gtttggagga agttgccaag tgtaaggcaa aaagcgagca
     2881 tcagagaccc agcaagatgc tgccagaagg attcggttag gaaagtaaaa atggcagatt
     2941 caaccggcca tgccgcaaag aaaaccatgc ttccccaaaa ccaagtcaat ttcccatcag
     3001 cttcatttct atttagaaaa aatgaaaccg cgacaagagc agcaaaacca atcagggcag
     3061 taataaagga cccatatttg ttaataaaat ccgatgttct cttattattt tccgtcaaat
     3121 cttctctcca acaagaccct ctttttcttt gccagtgatt ctcactggca caaagtcacc
     3181 caacccgact tttcctgtga ccctcacacg caaataagag ccggaaaaac cttcgctgtc
     3241 tgactcaacg aggactttag tgcaacgatt tatcatagca gaaattgcct gtcttgcaag
     3301 tgaagacgcc tttgctctgg catcttttgc ccttgccttg atgatttcgt ctggaactgc
     3361 ttccatatca tacgccttcg ttcccgggcg ctttgagaat gggaacacgt ggcatctgga
     3421 aaaagctgcc atttccatca ctttaatggt ttcttcaaaa tcattttcat tctccccagg
     3481 gaagccgacc agaatgtcgg tcgttatacc aatctcgtct ctaccttccc ttgccgcatc
     3541 aaccagctgc aaaaagtgct tctgattgta ccccctgttc ataaggccaa gcaccctgtc
     3601 ggagccagac tgcaaaggca agtgcaagtg tggcgcaacc ttttcgattg aaagcaattc
     3661 cctcaccagc gatggagaaa ggtgcctagg atttattgag gaaagtctta tgcgctcgat
     3721 tccccggacg ccagatattg attttataag gtctggcaga tcttttctgt gcccaagttc
     3781 agtaccgcac aaaacaatct ccctaaatcc ggaggatgcc aattttttag tttcctcaac
     3841 aatatccttt gccggtctgt tccttggttc ccctctcata tacggaacaa tgcagtacga
     3901 gcaaaaattg tcacacccaa gctggaccag aaccatcgcc ctagtgtgac caagaaatga
     3961 gtctatttca gatttcatgt tcccttgcaa accaagtttt tctgacaatt cctcaagagc
     4021 atcaggattg atatattcaa agccgtcttt tgcattgttt ttaccaagac accctgtcag
     4081 aaagaccctt gcaatggaag aaaatttcct ggcagtcctc atggcctctc tggaagctgt
     4141 ttcagttacc atacacccat tcacaaaaac aaggtcatac ggcggggaag tggcctcaat
     4201 gagcccaagc aaaatgaggt tttccctcaa taattccccc tgatactggt tgaccttgca
     4261 accaaaattg attacggaaa aattcaaatc aagcctcaac ctgtgatagc aactgggaga
     4321 tcgcaacaac aggggcaagc tctgccctta aaatccttgg gccaagtgtc accggtataa
     4381 aaccatttga tctggcaagc tctatttcag aatgttcaag gccaccttca ggaccaattg
     4441 ctattgtgac ctcagatggt tggcctttga tagattttgc tatgtgagtg ctttgctcaa
     4501 gttcccaagg gataagtttg gtaccttcgg aaagtgctat tgctttctca aaatccatcg
     4561 gtccgatgat ctctggcact atctccctgg cggaaagctc tgcagcacat tttgcggtta
     4621 tgccgaggct gtgtatcttt ttgtcccatc tgtcctgcgg gtaccttccc tgggttcttt
     4681 tgcaatatac aagaacaaac tttgaaac
//
LOCUS       DAOSRI010000137         4594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17038_length_4594_cov_3.989425, whole genome shotgun sequence.
ACCESSION   DAOSRI010000137 DAOSRI010000000
VERSION     DAOSRI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17038_length_4594_cov_3.989425"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PK717_04990"
     CDS             complement(<1..789)
                     /locus_tag="PK717_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOR47051.1"
                     /translation="MSYIHHEKISYWFDALLAFALGGLACLAVYGFQLTLSLGSYFNP
                     EKMSAIWVIAIPSLSGLIVGSVSYFLGISGAGAVVDTIAGANLKRRILAKPSSLLKPV
                     LAAISIVGGNPVGSGTVIMLGGTVAAFVGQYLNIPHRRRRLLIGCGAAAGIASVFLSP
                     LAGIAFVIEVVLMEYSPYTFSMLALSSGSAFLFSKIIGIKPLFPIPSFLAPRTIHLIV
                     FALIGISCAIVSFIWAKFMSYLERNRNKVKYWQIFGPLIGGLVTG"
     gene            975..1430
                     /locus_tag="PK717_04995"
     CDS             975..1430
                     /locus_tag="PK717_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR47052.1"
                     /translation="MNIGDTLPDFTLTDHNKKEWSKKLLEGKTALLSWHPLAWTKVCA
                     QQMQSLEEHANDFEKMGVIALGLSVDTVPSKRAWAKELGIEKTPLLSDFWPHGHFASL
                     LGIFRKDDGFSERTNIVVDGKGTIKFIKIYPISELPDIAEIIGGLGKWM"
     gene            1421..2011
                     /locus_tag="PK717_05000"
     CDS             1421..2011
                     /locus_tag="PK717_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR47053.1"
                     /translation="MDVKDAILARRAYRSLEKAEITDALVEDLIGFLKLAPSCFNNQP
                     WRFIFVKNPDVLLQMQQVMSKGNEWTFKDSMIVVVLSKKENDCVIYDREYHLFDTGMA
                     VGFMILRATELGLVAHPIAGYSPKKTRAVLGIPDDWQVITLVNVGKHSAELNPVMSEK
                     QVADEKTRPERLPNDEIASIDKFDPKLERTKPLQDS"
     gene            2023..2247
                     /locus_tag="PK717_05005"
     CDS             2023..2247
                     /locus_tag="PK717_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47054.1"
                     /translation="MKKKPASIQRATALHICDSVRTAALPKWWTTTYWGCKICMASSG
                     GVSEKLWISRRKGNRGCAFVNKAHLLRKKR"
     gene            2385..3074
                     /locus_tag="PK717_05010"
     CDS             2385..3074
                     /locus_tag="PK717_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR47055.1"
                     /translation="MPRENILVVDDEEDILELLRYNLAKNGFNVITATSGEDALKITK
                     TKTFDIILLDLMLPGIDGLDVCKEFKKNQSISSIPIIMLTAKGEEADIVAGLELGADD
                     YVTKPFSPRVLISRIRAVLRRRGKTVTDENSPLRYDEMEIDPRRFKVFVKSSLVELTH
                     TEFSILYFLAKKPGWVFTRYQIIDAIKGLDYPVTDRSVDVQVVGLRKKLGEYGDYIET
                     VRGVGYRFREL"
     gene            3071..>4594
                     /locus_tag="PK717_05015"
     CDS             3071..>4594
                     /locus_tag="PK717_05015"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOR47056.1"
                     /translation="MTSKTRGIFFYLFWPNAIAGLVFIILLSTITSIFLVNRYESTIF
                     DSLESKARIVQARYAKLLKSESVWELRQDVLQAGTGSQTRITVIGTGGNVISDSQADP
                     GQMENHKTRPEFESAMSGQTGKDIRSSYTLGKDMAYLAVPLATDGKIIGVLRLSQSIY
                     SIEILILIVFLSFLACYLVFCIVLILISKAISKKIVETIKQIAGKIKEMAGEDGRSKL
                     YIDAPAEFEMLSGAVNTAVETIQTMVNSANAGTAQQEAILSSMMEGVLAIDSSERIIR
                     IYKNAAVLLGVDEKQAGKSIHEAIRNSSLIKIVRKTLVSDSPVEEDFVLSGADGEKYI
                     MATGTTIKDRNSKVLGAVIVLSDVTKLRMFERMRKEFVANVSHELRTPVTSIKGSVET
                     LLDGAIDYPNDARKFLGIIASQAERLGSIINDLMSLSRIEQEEEKGLIQMEPHNLKTI
                     VLNALEDVQIKAKERSIQIDLVCERDITITANDRLLEQAIVNLIDNAINYSDPGSKIT
                     "
BASE COUNT         1495 a         1009 c         1053 g         1037 t
ORIGIN      
        1 tcccgtaaca agaccaccaa tcagcgggcc aaatatctgc cagtatttga ctttgtttct
       61 gtttctttcc agataggaca taaacttggc ccatataaaa gacacgatgg cgcaggaaat
      121 gcctatcaat gcgaatacta tcaggtggat cgtcctcggc gcaagaaaag acgggatcgg
      181 aaaaagtggc ttaatgccaa ttatctttga gaacaggaaa gcgctgccac tagatagcgc
      241 aagcattgag aaggtatatg ggctatattc catcagaaca acctcgatca caaaagcaat
      301 gccggctaaa ggggaaagaa atactgatgc aatgccagct gcagcaccac agccaatcaa
      361 caagcgcctt cttctgtgtg gtatatttag gtactgccca acaaatgccg caaccgtacc
      421 accaagcatt atgactgttc cggaaccaac aggattgcca ccgacaatcg agatcgcggc
      481 aagaactggt ttgaggagcg atgacggttt tgcaagtatc cttctcttga ggtttgcgcc
      541 agctatcgtg tcaacaacag ccccggcgcc acttattcca aggaaatatg aaactgatcc
      601 aacgatgaga ccactcaatg acggtatggc aatgacccag attgcactca ttttttcagg
      661 attgaagtaa ctgccaagac tcagcgtcag ctgaaatcca taaacagcca ggcatgcaag
      721 gccacctaat gcgaacgcca gcaatgcgtc aaaccagtag cttatctttt catggtgtat
      781 gtaactcacg ggaagttaga tacaccgctt gagcattttg acaataaaat atttggagac
      841 catattgctt taatataaat aaacacgcct aagcaagaaa aattgaagat tccaaaaaac
      901 ctgctacaac atataaataa agatgaattt gataatcaag actaatacca tataattcac
      961 ccaaaggagg aaaaatgaat attggagaca cactaccaga tttcacactg accgaccaca
     1021 ataaaaaaga atggagcaaa aaattgctgg aaggaaaaac agcccttctc tcctggcacc
     1081 cgctggcatg gacaaaagtt tgcgcccaac agatgcaatc cttggaagag catgcaaatg
     1141 attttgaaaa aatgggcgtt attgcactcg gcctgagcgt tgatacagtc ccaagcaaac
     1201 gcgcctgggc aaaagaactc ggaatagaga aaacgccgct cctgtcagat ttctggcccc
     1261 atgggcattt tgcctcgctg cttggcatat tcaggaaaga tgatggtttc tcggagcgga
     1321 caaatatagt tgtagatggc aaaggaacaa taaagttcat caaaatctat cctatatcag
     1381 aacttcctga tatcgcggaa ataattgggg ggcttggaaa atggatgtaa aagatgcaat
     1441 tttggcaaga agggcttaca gatcactgga aaaagcagag ataactgatg cactcgtcga
     1501 agatctgatt ggttttctaa aacttgcgcc atcatgcttc aacaaccagc cctggcgctt
     1561 tatttttgta aaaaacccgg atgtgctcct tcaaatgcag caggtgatgt ccaagggcaa
     1621 cgagtggacg ttcaaagact cgatgattgt cgtggtattg tccaaaaagg aaaacgactg
     1681 cgtaatatac gatagggaat atcacctctt tgacaccggc atggcagtag gattcatgat
     1741 actcagggcc acagaactcg gactggttgc acacccgatt gcaggataca gccccaaaaa
     1801 aaccagggca gtcctgggga tacctgatga ctggcaagtc attacgctgg tcaatgtagg
     1861 gaaacactcg gcagagctca acccggtcat gtccgaaaaa caggttgcag acgaaaaaac
     1921 aaggccagag aggctcccaa atgacgaaat agcaagtatt gacaagtttg acccaaaact
     1981 tgaacgcaca aaaccacttc aagacagcta aaaggagaca agatgaagaa aaagccagct
     2041 tcgatccaga gggcaaccgc cctgcacatc tgtgacagcg tgaggactgc tgccctgcca
     2101 aaatggtgga cgaccacgta ctggggttgc aagatctgca tggcaagttc cggtggtgtc
     2161 tccgaaaaac tctggatctc aaggcggaaa gggaacaggg gctgtgcttt tgtgaacaag
     2221 gcgcatttac tccgaaaaaa gcgctagact aaatccaaaa ccagtatcag acggtttttt
     2281 accagtttga cttcaaaaca acaaaaagat attattaatt tcctcacatt tttcttacaa
     2341 tcatcaataa taattagggc catcaaacaa ccggaaggta ggaaatgccc agggaaaaca
     2401 tactcgttgt ggacgatgaa gaagacatcc tggaactgct taggtataac cttgcaaaaa
     2461 atggttttaa tgtgattact gcaacttctg gggaagatgc gctcaaaata accaaaacaa
     2521 aaacttttga cataattttg cttgatctca tgctgcccgg aattgatggt ctggatgtct
     2581 gcaaagaatt caaaaaaaac cagtctatat cgtcaatacc aatcataatg ctcactgcaa
     2641 agggtgagga agcagacatt gttgcaggcc tcgagctggg cgccgatgac tatgtcacaa
     2701 aacctttcag tccgagagtc cttatctcaa ggatcagggc tgtgctcaga agacgcggca
     2761 agactgtaac cgacgaaaat tccccgctca gatacgatga aatggagatt gatccgagaa
     2821 gattcaaggt gttcgtgaag agttccctgg tggaactgac ccatacggaa ttttctatcc
     2881 tctacttcct cgccaaaaaa cccggttggg tcttcacaag ataccagatc atagacgcaa
     2941 taaaggggct tgattatccc gtaacagaca gatcggttga cgtccaggtt gttggcttga
     3001 ggaaaaaact tggagaatac ggagactaca tcgaaaccgt caggggagtt ggctaccggt
     3061 tcagggaact atgaccagta aaacaagggg cattttcttt tatctgttct ggccaaatgc
     3121 aatcgcagga ctggttttta ttatcttgtt gtccactatt acttcaatat tcctcgtgaa
     3181 ccgctatgaa agcacgatat tcgactctct cgaatcaaaa gcaaggatcg tccaggccag
     3241 atatgcaaaa ttgctgaaga gtgaaagcgt ctgggagttg aggcaggatg ttcttcaggc
     3301 aggcaccggc tctcaaacaa ggataacagt cattgggaca ggtggcaatg taatctcgga
     3361 cagccaggca gaccctggcc agatggaaaa tcacaaaaca agaccggaat tcgagagcgc
     3421 catgtctgga caaacaggaa aagatatcag atcgagctat acgctgggca aagacatggc
     3481 ctacctggct gtgcctcttg caacagatgg aaaaattatc ggggtactaa ggctttcaca
     3541 atctatttat tcaatagaaa tcctcatttt aatagttttt ctttccttcc ttgcctgtta
     3601 tttagttttt tgcattgtac tcattttgat ctccaaggcc atatcaaaaa aaatcgtaga
     3661 gacaataaaa caaattgctg gaaaaatcaa agaaatggcc ggagaggacg gaagatcaaa
     3721 gctttatata gatgctccag ccgaattcga gatgctctct ggagctgtaa acacggccgt
     3781 ggaaacaatc cagacaatgg tgaattctgc aaacgccggg accgcacaac aggaagcaat
     3841 cctgtcatca atgatggagg gtgttctcgc aatcgacagc tccgagagaa taatcaggat
     3901 atacaaaaat gctgcagtac tgctcggagt tgatgaaaag caggccggca agtcaataca
     3961 cgaggcaata agaaactcat cgttgataaa aatcgtccgc aaaacacttg tttcagattc
     4021 accggtagaa gaggattttg tgctgtcagg ggctgatggc gaaaaataca tcatggccac
     4081 cgggacgacg ataaaggaca ggaactcgaa ggtcctcggg gcagtaattg ttctctcgga
     4141 cgtaacaaaa ctcaggatgt ttgagagaat gaggaaagaa tttgtagcaa atgtttccca
     4201 cgaactaaga acaccggtca cttcaattaa aggttctgtg gaaaccctgc tagacggtgc
     4261 aatcgattat ccaaacgacg caagaaagtt cctcggcata atcgcaagcc aggccgagcg
     4321 tctggggtca ataatcaatg acctgatgtc actctccaga atcgagcagg aagaagaaaa
     4381 gggcctgata cagatggaac cacacaatct gaaaacgatt gttttgaatg cccttgagga
     4441 cgtccagata aaagcaaaag aaaggtcgat ccagattgac cttgtatgcg aaagggacat
     4501 caccattaca gcaaatgaca ggttacttga acaggcgatt gtcaacctta ttgacaatgc
     4561 aataaactac agcgaccctg gctcaaaaat cacc
//
LOCUS       DAOSRI010000138         4588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17072_length_4588_cov_3.998235, whole genome shotgun sequence.
ACCESSION   DAOSRI010000138 DAOSRI010000000
VERSION     DAOSRI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17072_length_4588_cov_3.998235"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PK717_05020"
     CDS             <1..626
                     /locus_tag="PK717_05020"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOR47057.1"
                     /translation="VFAKGILNIHPSLLPRYRGAEPIRWAIRKCEKETGVSLMLISNR
                     LDRGNVIASETIAIEDTDDFGSLSEKLAKLSVSMLPEAIKKLGQGFEGEPQSQEKTFY
                     ARRLKPDDEVINWNKTAFELSCHIRSMSPDPGCYTVLDGKRIKLFGPKVVEGNGQPGE
                     VLLSKKTLIIACKEGALEFTKCQKEGGKLLDTRDFLASGLIRQGLVF"
     gene            669..1424
                     /locus_tag="PK717_05025"
     CDS             669..1424
                     /locus_tag="PK717_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR47058.1"
                     /translation="MRYGIISDIHANIEALNVVLASLEKENVDEIICCGDIVGYNANP
                     DECVAKIINLGVRAIMGNHDKALIEPSEERYFNQYGLSAIRWQRTQVTPSHQKFISNL
                     PMKLKIPGPNSHDGFQVAHGSLDSSAPFEYIASPMEAVRLSEIMSSNICFIGHSHISG
                     VFSIKGHDDIEYQRGSYGLEISLEPGIKYIINVGSVGQPRDRNPQASYAVYDTDMRKA
                     TIKRVDYDIEATIAKVMEAGLPLFLADRLRIGW"
     gene            1467..2252
                     /locus_tag="PK717_05030"
     CDS             1467..2252
                     /locus_tag="PK717_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47059.1"
                     /translation="MKRYAIYSLALVFLLLSITVATTQEQSRTIVMKIGEKIATVDDK
                     SVTLQPAPTIIEERTVVPLRFLIDNVFPKDGSSVEYDTKEKKITITIPDISVLKAENL
                     SLQDENRKLKQALADCIKNAEEGGGGSTENLVPPIKFSKNGVDFTLVAIVKEKNNLRL
                     DIKIYNKLETKVRFPASLTKMIVGQEKLQASDWDPIFSSDIPSGAERSGWVRLPLKTL
                     TGVATFEFTVWPKDVLLYYTFQLKVNLDQAIPTSVPPSTSLPR"
     gene            complement(2188..3519)
                     /gene="radA"
                     /locus_tag="PK717_05035"
     CDS             complement(2188..3519)
                     /gene="radA"
                     /locus_tag="PK717_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOR47060.1"
                     /translation="MAKKTISYACSNCGYSSTKPLGKCPECGAWGTFEEKEMFPQDSS
                     KCKDIEVEGISLAEENRIETGITEFDRVMGGGVVQGSVVLIGGEPGVGKSTLMLAVAN
                     SYAKTSQSTLYCSGEESPKQISMRAKRIGVMDSGISLVFETDLDAILNEAAENLPKAL
                     FIDSVQTIMLEDAGTPGGPNMLREATRKIVEFSKKHSVTTFLIGHVTKEGNIAGPKTL
                     EHLVDVVCYLEGERYSTLRILHGVKNRFGATDEVGLMEMTEGGFRAADRQSLVRNENV
                     PGVAITAVSKGNRPIALEVQALVAPTYYPSPRRVVTGCNMNRALMLLAVLEKRASIPL
                     GKLDCYVNLVGGLDSDEPSLDLPISFALVSSFMDRKVPEGVAFVGEVGLTGEIRPVSQ
                     LGTKLKELNALGLKTVLVPNQDKPDIEGLEIISAGTLREALKLVWPGQGLP"
     regulatory      3537..3729
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3736..4557
                     /locus_tag="PK717_05040"
     CDS             3736..4557
                     /locus_tag="PK717_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOR47061.1"
                     /translation="MKKILSVLLLAFMLCSCGVQPAQPSANAPEKIISLSPNITEIVY
                     FVGAGDRLIGRTSQCNYPPEVAKVESLGNPYALKIERVVELLPDVVLVNRLVPLDVIN
                     KIKSSGIRVETFDPQTVNDVLDTIDKVADICGLPAKSQELRPEIESLHKETAKGSKKL
                     VYLEIWDNPPTTFGKNSLGADMVRWVGAENAGDRLDGDFPTLSEEAIAGLDPEVIIVP
                     ESYKKTVAEIEKRQGVAKTRAAKNGSIYIMNDDLLMRPSPRVLDALKIISQIVGK"
BASE COUNT         1317 a         1107 c         1064 g         1100 t
ORIGIN      
        1 atgtttttgc aaaagggata ctcaacattc atccatcact actcccaaga tacaggggtg
       61 cagagccgat aaggtgggcc atcaggaaat gtgagaagga aaccggtgtg agcttgatgc
      121 tcatctcaaa cagactcgac aggggcaacg taattgcttc agagacaatc gcaatagaag
      181 acacagatga ttttggttcc ctttcagaga agctggccaa gctttctgtt tcaatgctgc
      241 cggaagcaat caaaaaactt gggcagggtt ttgaaggaga gccacaatcc caggaaaaaa
      301 ccttctatgc aagaagactt aaaccagatg atgaagtcat caactggaac aagacggctt
      361 ttgagttgtc ttgtcacata agatcaatgt cgcctgatcc cggttgctac acggtgcttg
      421 acggcaaaag gataaaactg tttggcccta aagtggttga aggcaatggg cagccagggg
      481 aagtgcttct ctcgaaaaaa acattaataa tcgcctgcaa ggaaggggcg ctggaattta
      541 caaaatgcca gaaagaggga gggaaattgc tcgatacccg ggacttcctg gcatcaggtt
      601 taataaggca gggactggtt ttttaacctg tgttgactaa atgacctcta cgggtaaaat
      661 aaaacaaaat gagatacggc ataatttctg atatacacgc caacatcgaa gcattaaacg
      721 ttgtgcttgc ctcactggag aaagaaaacg ttgacgagat aatctgttgc ggggacatcg
      781 tcgggtacaa tgcaaatccc gatgagtgtg ttgcaaaaat aataaacctt ggggtaaggg
      841 caataatggg caaccatgac aaggcgctta tagagccctc tgaggaaaga tatttcaatc
      901 aatatggcct ctctgcaatc aggtggcaga gaacacaggt aactccctcg caccaaaaat
      961 tcataagcaa tttaccaatg aagctgaaaa taccagggcc aaactcacat gatggtttcc
     1021 aggtcgccca tggtagtctg gattcatccg cacccttcga atacatagct tcgccaatgg
     1081 aagccgtcag actctcagaa ataatgagct caaatatatg ctttatcggg cattcccaca
     1141 tatccggtgt tttttcgata aaaggccatg atgatattga ataccagagg ggttcttatg
     1201 gtctggaaat atcgcttgag cccggcatca aatacataat aaatgtcggg tcagtcggcc
     1261 agccaaggga caggaatccc caggcaagct acgctgttta tgacacagac atgagaaaag
     1321 ccacaataaa gcgcgtggac tacgacattg aggcaaccat tgcaaaagta atggaggctg
     1381 gacttccact tttcctcgcc gacaggctga ggataggctg gtagagtatt gacataaaaa
     1441 tcataatgac tataatacgt cccgccatga aaaggtatgc aatttacagt ttggcgctag
     1501 ttttcttact cttgtcgata acggttgcta caacccagga gcaatcgcgc acaattgtaa
     1561 tgaaaatcgg tgaaaaaatc gcaactgtgg atgacaagtc agtcaccctt cagccagcgc
     1621 caacaataat tgaggagagg acagtcgttc cactccgttt ccttattgac aatgttttcc
     1681 cgaaagatgg ttcctctgtg gagtatgaca caaaggaaaa gaaaataaca attacgatcc
     1741 cggacatctc ggttcttaaa gcagaaaatc tttctcttca ggatgaaaac cgcaagctca
     1801 aacaagccct tgccgattgc atcaaaaatg ctgaagaggg cggcggcggt tcaactgaaa
     1861 atcttgttcc gccaataaaa ttctccaaaa atggcgttga tttcaccctt gttgcaatcg
     1921 tcaaggaaaa aaacaacctc aggctcgaca tcaagatcta caacaagctt gaaacaaaag
     1981 tcaggttccc ggcctcactc acaaaaatga ttgtcggcca ggaaaagctc caggcctcag
     2041 actgggaccc tattttctca agcgatatac catcgggtgc tgaaagatcg ggttgggtaa
     2101 ggcttccttt gaagaccctg actggagtgg ccacgtttga attcactgtc tggccaaaag
     2161 acgttcttct gtactacacc ttccaactca aggtaaacct tgaccaggcc ataccaactt
     2221 cagtgcctcc ctcaacgtcc ctgccgagat aatctccagc ccttcaatat caggcttgtc
     2281 ctgatttggc accaacacag ttttaagccc caaagcattg agttctttta gttttgttcc
     2341 caattggctg acagggcgta tttcaccagt aagccctacc tcaccaacaa aagccacgcc
     2401 ctcgggcact tttctgtcca taaacgaaga cacaagcgca aaagatattg gcaggtcaag
     2461 tgatggttcg tcagaatcca gaccaccaac aagatttaca tagcaatcca gtttccccag
     2521 aggaatagag gccctttttt ccaggacagc cagaagcatc agcgccctgt tcatgttgca
     2581 accagtcacc accctccttg gggaaggata gtatgttgga gccacaagcg cctggacttc
     2641 aagcgctatc ggcctgtttc cctttgaaac ggcggttatt gccaccccag gaacattctc
     2701 attcctgaca agcgattgcc tgtctgctgc cctgaagcca ccctcggtca tttccatcaa
     2761 accaacttca tctgtcgcac caaagcggtt ttttacacca tgtagtatcc ttagggttga
     2821 atatctttcg ccctcaaggt agcaaaccac atcaacgagg tgctcaaggg tctttggccc
     2881 tgcaatgttg ccttcttttg tgacatggcc aataagaaag gttgttacag aatgcttttt
     2941 tgagaactcg actatttttc tggtcgcttc cctcagcatg tttggcccgc caggggtgcc
     3001 tgcatcctca agcattattg tctggaccga gtcgatgaac agtgcttttg ggaggttttc
     3061 tgcggcctcg ttcaggattg catcaagatc tgtttcaaaa accagcgaaa tgccagagtc
     3121 catcacccca atgcgttttg ccctcatgga tatctgcttt ggggactcct cgccagaaca
     3181 atagagggtg ctttgggaag tttttgcgta gctgtttgcc accgcgagca tgagcgtgga
     3241 tttgccaaca cctggttctc ctccaatgag aacgaccgac ccctggacaa caccaccacc
     3301 catgactctg tcaaattctg ttatgccagt ttcaatcctg ttttcttcgg caagagaaat
     3361 gccctccacc tcaatgtctt tgcatttgga ggagtcctgc gggaacatct ctttctcttc
     3421 aaatgttccc catgcaccac attcggggca ttttccaagc ggtttcgtgc ttgaataacc
     3481 acaattcgaa caggcataac ttatcgtttt tttggccaca ttctggattg tatgttggca
     3541 ttcaatcttg acaaggacca atgcgaggct agactcgacc atgcaaaggc agcggaagcc
     3601 ggtgagaatc cggcgcggtc ccgccactgt aagcggcaag tctggggaat ccactgggca
     3661 accgggaagg aaaccccgca aggcgccaag ccgcaagcca ggagaccgtc tttgcgaaaa
     3721 cccgaggagg taaatatgaa aaagatattg tctgttttat tgctggcatt catgctctgt
     3781 tcttgtggag tccagccggc ccagccttct gccaatgccc cggagaagat catctccctt
     3841 tcaccaaaca tcacagagat agtttatttt gttggtgcag gcgacaggct cattgggaga
     3901 acctcacagt gcaactatcc cccggaagtt gccaaagtgg aaagccttgg aaatccgtat
     3961 gcactaaaga tagagcgtgt agtcgagctt ttaccggatg tcgttcttgt aaacagactt
     4021 gttccgcttg atgtcataaa caagataaaa tcatccggaa taagggtcga gacctttgat
     4081 ccgcaaaccg taaacgatgt tctggacaca atagacaaag ttgcagacat ctgtggtctg
     4141 ccggcaaagt cccaggagct caggccagaa attgaatcgc tccacaaaga gacagcgaag
     4201 ggcagcaaaa aactggttta tctcgaaata tgggacaacc cgccaacaac atttggcaag
     4261 aattcacttg gcgccgacat ggtaaggtgg gttggagcag agaatgctgg cgacaggctt
     4321 gatggcgatt tcccgacgct ttcagaagaa gcaatcgccg gccttgatcc agaagtaatc
     4381 attgttccgg aatcatacaa aaagacggtt gccgaaatcg agaagaggca aggtgtcgcc
     4441 aaaaccaggg ccgccaaaaa cggctcgata tacataatga atgatgatct tttgatgagg
     4501 ccaagcccaa gggtgctaga cgcactgaag ataatctcgc agattgtcgg caagtgaagc
     4561 aaaccagaat aatactgtta ctcgtttt
//
LOCUS       DAOSRI010000139         4527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17351_length_4527_cov_3.900716, whole genome shotgun sequence.
ACCESSION   DAOSRI010000139 DAOSRI010000000
VERSION     DAOSRI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17351_length_4527_cov_3.900716"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /gene="nuoL"
                     /locus_tag="PK717_05045"
     CDS             complement(<1..1843)
                     /gene="nuoL"
                     /locus_tag="PK717_05045"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOR47062.1"
                     /translation="MEHTVASLAGSWTLTSILAVLTMAFPLLGFIIVGLCGKIAKEWG
                     PKLATISIFISLITSIVVLGLVIANGPAPTVSFVWLQSGNLTLDFGFRVDQLCAMMLC
                     VVTLVSFLVHLFSQDYMHGDPGIARFYAELQLFTTAMLGVVTASNLLQLFAFWELVGL
                     CSYLLIGFWFHKRSAYQAAKKAFFVTKVGDIGLFTGIIMCLILSNPHTLNLEALPSAI
                     ASLAPALLIIIPLLIFMGAMGKSAQFPLHVWLPNAMEGPTPVSALIHAATMVAAGIYL
                     VARCMGLFTLDSSGIALKVVTYVGSFTAIFAATIATVQDDIKKVLAYSTISQLGYMVM
                     ALGFGKFGYTAALFHLMTHAMFKGLLFLGSGSVIHATHTQNMHEMGGLFKKMPITAWT
                     FILGSLALAGLFPFAGFWSKDAILVGAEATHSTIPLILALITAFLTPYYMTRCVWLTF
                     FGKPRKENHVHEASWKTTLPLIILAVLSVVIGFVGAPFFGELFQRFIFFHEFESEAVN
                     WTLIFGSTGLVLAGIFLGLAIYAWGWFKKETLIRVLKPLYLFFKNKWFIDEAWTFIAV
                     KPLFYVSEFCHWFDRTVIDGAVNGMAWLSVVLGNIVYWFDAGIVDGLV"
     gene            complement(1852..2151)
                     /gene="nuoK"
                     /locus_tag="PK717_05050"
     CDS             complement(1852..2151)
                     /gene="nuoK"
                     /locus_tag="PK717_05050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOR47063.1"
                     /translation="MNYLLLILGAAIFSFGVLGALTLRNAIRILMCIELMLNGANLTF
                     IATASQLPDPGMAQSMVVFAIAIAAAETIVGLGAIVAMYRAKGWVTLEQFSLLKW"
     gene            complement(2148..2693)
                     /locus_tag="PK717_05055"
     CDS             complement(2148..2693)
                     /locus_tag="PK717_05055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOR47064.1"
                     /translation="MNVTMSQALYMALAFPALVFAALTVTVKNIVHAAICLIGCLLSI
                     AAIYVGLHADFIAAAQIAIYVGAIAILILFSIMFTHDVSGKQMSHLNRQAVLGVFVAL
                     ALFGAISFFIMNTSESQLTTNFQGQMALPPKTSLMAVGDALYSDNASTPYMVPVEMAS
                     FLLLAAVVGGIEMAKKKEDEV"
     gene            complement(2690..3106)
                     /locus_tag="PK717_05060"
     CDS             complement(2690..3106)
                     /locus_tag="PK717_05060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOR47065.1"
                     /translation="MYGTGIASSLWVTVKNIFRVKITLRYPKQKQAMVPRFFALMRMV
                     RDENGREKCTACGICANNCPTHAITIEKGKREDNTPFPVKYSVNVQQCIFCGICVEVC
                     PFGALQVGHKYELSSYTREGMIHEKEELLLPFGGAQ"
     gene            complement(3114..4133)
                     /gene="nuoH"
                     /locus_tag="PK717_05065"
     CDS             complement(3114..4133)
                     /gene="nuoH"
                     /locus_tag="PK717_05065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOR47066.1"
                     /translation="MENFVPYLIVALIQVAISLGIFGFVMVNALLLTLAERKLLGWMH
                     QRLGPMEVGPWGLFQPFADMIKLLTKGDVIPKAADKFFYSYSALIAFTPSFITFAFIP
                     FGLVGGIKLVAADTEIGVLMISAVMALSVIGIILGGWSSGNKFSLLGAVRTAAQEISY
                     EIPKTLAIIAVVMLAGSMNVYHISSIQRVWFIAPQFIGFLVFFVSSLAEIFRIPFDLA
                     EGESELVAGYNVEFSGIRFAFFFFAEYTYLFVTSAIITALYLGGGSGPEFLPWGLSSL
                     FWFLLKSFAMVGFILWFSRSLPRVRADQLMNMAWKVMLPLSLVNIILTAYVVLNENWL
                     KVFVK"
     gene            complement(4138..>4527)
                     /locus_tag="PK717_05070"
     CDS             complement(4138..>4527)
                     /locus_tag="PK717_05070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOR47067.1"
                     /translation="IYPTLDFDVISENTCDSYGRFLVRFKEIEQSVRIIEQIMPNLPE
                     GPVMAKVPKVIKPPAGEIYCRVEAPIGEFGVYLVSDGSSKPYRVKLRSPSFSNLMSFP
                     HIAQNISMPDVVAAFASLAPTMGDSDR"
BASE COUNT         1261 a         1065 c         1146 g         1055 t
ORIGIN      
        1 tgaccagacc gtcaactatt ccggcatcga accaataaac gatgttgcca agaacaaccg
       61 agagccatgc cattccattg actgccccgt caatgactgt ccgatcaaac cagtggcaaa
      121 attcactcac atagaaaagc ggcttcactg cgatgaaagt ccaggcctca tcaataaacc
      181 acttattctt gaaaaagagg taaagtggtt tgaggaccct tatgagtgtt tcttttttga
      241 accagcccca ggcgtagatt gcgagcccaa ggaagatgcc tgcaagaaca agccctgtgg
      301 agccaaaaat gagcgtccag ttgactgctt cggattcgaa ctcatggaag aatataaacc
      361 tctggaagag ttcaccaaag aacggtgccc cgacgaagcc tatcaccact gaaagcacgg
      421 ccagtattat tagcggaagg gttgtcttcc acgaggcttc gtggacatgg ttttcttttc
      481 tgggttttcc aaagaaggtc agccagacac atcttgtcat gtagtatggg gtgaggaagg
      541 cggttatcag tgcgaggata agcggtatgg tcgagtgggt tgcctctgcc ccgacaagta
      601 ttgcgtcttt actccaaaac ccggcaaacg ggaagagtcc cgcaagggca agcgatccaa
      661 gtatgaaagt ccacgcggtg atgggcattt ttttgaacag tccgcccatt tcatgcatgt
      721 tttgggtatg tgttgcgtga atgaccgagc ctgagccaag gaagagcaaa cctttgaaca
      781 ttgcgtgggt catgaggtgg aagagtgcag cagtgtagcc aaatttgccg aaacccagcg
      841 ccattaccat gtaacccagt tgactgatgg ttgaataggc aagaaccttt ttgatgtcat
      901 cctgcaccgt ggctattgtt gcagcaaaaa ttgccgtgaa agagccaaca taagtgacga
      961 ccttcaaagc aatcccactg gaatcaagtg tgaacagccc catacacctt gcaacaagat
     1021 atataccggc cgcgaccata gttgcggcat ggataagcgc agaaactggt gtcgggcctt
     1081 ccatggcgtt ggggagccag acgtggagcg ggaactgggc agatttgccc attgcgccca
     1141 taaaaatcag gagtggtatt attatgagaa gagcaggggc gagtgatgca attgcagatg
     1201 gcagggcctc aaggttgagc gtatgagggt ttgagagtat gaggcacatt attatgcctg
     1261 tgaagaggcc aatatctcca acttttgtaa caaaaaaagc cttctttgcg gcctggtatg
     1321 cggacctctt gtggaaccag aagcctatga gaagatatga acagagacca accaactccc
     1381 aaaaagcaaa gagctggaga aggtttgatg cggtaactac gccgagcatt gcagtcgtga
     1441 agagctggag ttcggcatag aaccttgcaa ttccaggatc accgtgcatg tagtcctggg
     1501 agaacaggtg cacaaggaac gataccagcg tgaccacaca aagcatcatt gcacagagct
     1561 ggtcgaccct aaacccgaaa tcaagcgtca ggttgccaga ctggagccag acaaagctga
     1621 ctgttggtgc tggcccgttt gcgataacga gtccaagcac cacaatcgag gttatcaatg
     1681 aaataaagat gcttattgtt gcgagctttg gtccccactc ctttgctatt tttccgcaga
     1741 ggccaacaat gatgaatccc agaagtggaa aggccatcgt taaaacagcg aggatcgagg
     1801 tgagggtcca ggaaccggca agggaagcta cagtgtgttc cattgtccct cctaccactt
     1861 gagcaggctg aactgctcga gagttaccca gccctttgcc ctgtacattg ccacaattgc
     1921 accgagtccg acaatggttt cagctgctgc aattgctatg gcaaacacga ccattgactg
     1981 cgccatgcct ggatcgggga gctggcttgc cgtagcgatg aaggtgaggt tggccccatt
     2041 gagcatcaac tcaatgcaca taagtatcct gatggcgttt ctcagggtga gggcgccgag
     2101 gacaccaaag ctgaatattg cagcaccaag gataagcaag aggtagttca tacctcgtcc
     2161 tcctttttct ttgccatctc tattccgcca acaacagccg caagcagaag gaaagaggcc
     2221 atttcaacag gcaccatgta aggtgtggaa gcattgtctg aataaagtgc gtcaccaact
     2281 gccatcaatg atgtttttgg tgggagggcc atttgtccct ggaagttagt tgttaactgg
     2341 ctttccgagg tgttcatgat gaagaaggag attgcaccaa agagcgcaag ggcgacaaaa
     2401 acaccaagga cggcttggcg gttcaggtgg ctcatctgct ttccagagac atcatgcgtg
     2461 aacatgatcg agaacaggat gagtatggct atagcgccaa cgtagatggc tatctgtgct
     2521 gcagcgataa aatcggcatg caaccctaca tagattgccg caattgacag gaggcaacca
     2581 atcagacata tggccgcatg gacaatattt tttacagtta cagttagggc cgcaaagaca
     2641 agtgcgggga aggcgagtgc catgtaaaga gcctgtgaca ttgttacatt cactgtgcac
     2701 ccccaaacgg aagaaggagc tcttctttct cgtgtatcat cccttccctt gtgtatgagg
     2761 aaagctcata tttgtgacca acctgcagtg caccaaacgg gcaaacctcg acgcatatcc
     2821 cacagaatat acactgctgg acatttacac tgtacttaac cgggaaaggc gtgttgtctt
     2881 cccttttgcc tttttcaatg gtgatcgcgt gtgttgggca attgtttgcg caaattccgc
     2941 aggcggtgca tttttccctg ccgttttcat cgcggaccat tctcatcagg gcaaaaaacc
     3001 ttggcaccat cgcctgcttt tgctttgggt agcggagcgt tattttgacc ctgaatatat
     3061 ttttaacagt gacccagaga gagcttgcta ttcctgttcc gtacatgatc acctcatttc
     3121 acaaaaacct tgagccagtt ttcatttaaa acaacatatg cggtcaagat tatattgacg
     3181 agagagagcg gtagcatgac tttccatgcc atgttcatga gctggtcagc cctgaccctt
     3241 ggcagcgacc tggagaacca gaggatgaaa ccaaccatag caaaggactt caggaggaac
     3301 cagaacagtg aagacagacc ccatggaagg aactctgggc ctgaaccccc accaagatag
     3361 agcgcagtaa tgattgctga agtcacaaag agatatgtgt attctgcaaa gaaaaagaac
     3421 gcaaacctga tgccggagaa ttcaacgtta tatccggcca caagctccga ttcaccttct
     3481 gcaaggtcaa atggtatcct gaatatctca gcaagtgaag aaacgaaaaa taccaggaaa
     3541 ccaatgaatt gtggggcgat aaaccaaact ctttgtatcg aagagatgtg atatacattc
     3601 atactgccgg cgagcattac cactgcgata atcgccagtg tttttgggat ttcatagctg
     3661 atttcctggg cagcagtcct gacagcaccg aggagcgaga acttgtttcc ggatgaccac
     3721 ccgccaagga ttatgccgat gacagacagc gccatgacgg ccgagatcat cagaacaccg
     3781 atttcagtgt ccgcggcaac aagctttatt ccgccaacaa gtccaaacgg aatgaaggca
     3841 aaggtgataa aagatggggt gaatgctatg agggcagagt atgaatagaa aaatttgtca
     3901 gctgcctttg ggataacatc accttttgtc agaagcttta tcatgtcggc gaaaggctgg
     3961 aagagacccc atggcccaac ttccatcggg ccaagtcgct ggtgcatcca gccaagcagt
     4021 tttctttcgg caagggtcag aagtagtgca tttaccatta cgaacccgaa aatgcctagc
     4081 gatattgcta cctggataag tgctacaatc agatatggga cgaagttttc cattttgtca
     4141 cctgtccgaa tcgcccattg ttggcgccag cgatgcaaat gcggcgacga catcaggcat
     4201 ggagatattt tgggcaatat gtgggaacga catcagattg ctgaagcttg gtgaacggag
     4261 ttttactcta tatggctttg aagagccatc agaaacaagg taaacgccaa attcaccgat
     4321 tggagcttca accctgcagt atatttcacc ggccgggggc ttgatcacct ttggcacttt
     4381 ggccattaca ggcccttccg gaaggtttgg cattatctgc tcgatgatcc taacggactg
     4441 ctctatctcc ttgaatctca ccaggaagcg tccgtatgaa tcgcatgtat tttcacttat
     4501 tacatcaaaa tccagcgtgg ggtatat
//
LOCUS       DAOSRI010000140         4523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17373_length_4523_cov_3.893241, whole genome shotgun sequence.
ACCESSION   DAOSRI010000140 DAOSRI010000000
VERSION     DAOSRI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17373_length_4523_cov_3.893241"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..3302
                     /locus_tag="PK717_05075"
     CDS             222..3302
                     /locus_tag="PK717_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47068.1"
                     /translation="MKALSFSVCLALVLQVLAVPQALCADISSQVTISALGEARKARV
                     EFGFESDSDASVYFDRETKDYETIFTSDADSTKPVDVKSLEMKHDSLYVWARIMLYKP
                     VDGDVFISIAMDVDGNPKTGMPKANDRSYNQGGEDFFARYSREHGADKFVLDRWVGPD
                     TVETGGLGFGTVKGDTISFAIPRRAIGEPSNIDFKLVARTFQDTNPDLSPDHLKYTMV
                     GAKASIEAKTEKAKGKTFAYFDFPAQVSVGIHRVHVGLSGKFACVANYVEAEQKVTPP
                     WQLLARNPPVLFKRMCIKNIFLMQDQERVHFRIDFWDGLSGMGNYDKKILVLADTDPK
                     TGGFSNERFCKGNEDYIFEVATESGRLVSNVWNYKEGIGGFRNSPLTDLVCNAESGTV
                     QFSVGLKRIGTPKNISFRVACGGWKAPEGDWDIAGPVSSSLVQSDTPVFTTIFEDNED
                     PSFPVDMKTIECANNPSKGNVLFKVGFYKDPTASGNDVQVSIFINSDQDASTGMFPEN
                     GSPGGEDFIVNVYMKGGNVICRLLSAQNGPISEVAELEGTVFTDGKLLVPVPLEMIGN
                     PKSLSFYVVCMSSVNPMAQDKSPFDLAFTITGEMVEKTCPTTVKVSVKPGDSAATVMW
                     SEPSTQANGFLVYRIEGNNAPKLLTPIPLPANARQFEDTGLTNGMSYSYYVRVTCPDG
                     SLGPSSALATTVPRKQEQPYPKIEIAPTSINLGSMQNTKGAYATFKVKNSGPGDFSCE
                     IKPEPDYLAALPDKLVLQQGKSADIRIEVTKDLATGQHFGRIMVKSAIGTLYIEISLN
                     VPEIGPQFKFVSNLIAEPMPFAIKLSWLPPTYRGEELKGYRIERTEYYRGKLLPEKKI
                     FESPSGKNEFLDSDLDPQSVYNYKVVPVWPSGDGIPRMAESSPSAPQVVIMMKVGIKT
                     AVVNDSTVELDAPPYITKGKTMVPFRFIGENLFAKVDYDDYTKTVTFKRGRTTVRIKI
                     GESKAEVNGTLMTMDPPATIKDGRTFIPVRFVSDALGAKTLWEASARQVVITYP"
     gene            3495..>4523
                     /locus_tag="PK717_05080"
     CDS             3495..>4523
                     /locus_tag="PK717_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47069.1"
                     /translation="MNISLGDEFTIARSSSDEYSPFINSDFAIFSEKSGGITSAKSFN
                     LPDGSLSAIFDDKNGAPSFPSDELCYIGQDYCVTLKNSNCSSSSSWISAYNFKSKTTT
                     TISDTPATRDCVSIWDKYAVWTETDVSNPGIYIADITNPSGKTRIYDFIVNDQWCWPV
                     VYDGKVAYESFDGADSDIKLYDIKTKITYSIGDPAVFEKNPVIQGDYVYYTSCSLSLD
                     SFGKIKGHKISTGETFDVWNNPDDYFLTLMFSPESEILVVKVFNFDNGREKLMGFEPV
                     KSMLFDITPWLQEDCISIVSECGFGRKIVWIENRRSENEQWYKTPGVRLAIMDSNGNL
                     KIFDITGQN"
BASE COUNT         1311 a         1017 c         1097 g         1098 t
ORIGIN      
        1 aaaaaccacc atattgttct gggtttcctc ggacttttgc acgaggtggg acgggacaag
       61 ggaaagattt ttggtatgct gaccctgtgc accgcaggag agcataaaaa cggctgaccc
      121 gaaaagtgct aaaataattt tgaacctgtt tgccatgtaa gatagtttta tgtgtagaga
      181 cccaaaggtc aatgatgtgc aaacttttgc cggaggtttt tatgaaagcc ctcagctttt
      241 ctgtgtgcct tgctcttgtg ctccaggtgc ttgctgtgcc gcaagccctc tgcgccgata
      301 tttcatcaca agtgaccatt tctgcgcttg gcgaggcgag aaaagcaagg gtggagtttg
      361 gttttgaatc agattcagac gcttccgtat attttgatcg cgaaacaaaa gactacgaaa
      421 caattttcac ctccgatgcc gatagtacca aaccggttga tgtgaagagc ctggagatga
      481 aacatgacag cctgtatgta tgggcaagaa taatgctata caaaccagtt gatggcgatg
      541 ttttcatctc catcgcaatg gacgtggatg gcaacccaaa aaccggcatg ccaaaagcaa
      601 acgacaggtc atacaaccag ggtggggagg atttttttgc caggtattcg agggaacatg
      661 gcgctgataa gtttgttctg gacagatggg tcgggccaga tactgtcgag acaggcgggt
      721 tggggtttgg aacagtaaaa ggtgatacca tctcttttgc catcccgaga agggcgattg
      781 gcgagccatc aaatattgat tttaagcttg ttgcgaggac attccaggac accaacccgg
      841 acctttctcc agaccacctc aaatacacga tggtaggcgc aaaagcctcc attgaagcaa
      901 aaacggaaaa ggccaagggc aaaacctttg catattttga ctttccggca caggtttcgg
      961 tgggcatcca cagggttcat gtcgggctct ccggaaagtt tgcctgcgtg gcaaactacg
     1021 tcgaggccga gcagaaggtc acgccgccct ggcagctgct cgcaagaaac ccgccagttc
     1081 tcttcaaaag aatgtgcatc aaaaatatct tcctgatgca ggaccaggag agggtgcact
     1141 tcagaataga cttctgggac ggactttccg gaatgggtaa ctatgacaaa aaaatacttg
     1201 ttttggccga tactgacccc aaaaccggcg gattttcaaa tgagaggttt tgcaaaggca
     1261 acgaggacta catttttgag gttgcaacag aatctggcag gctcgtttca aatgtgtgga
     1321 actacaagga aggtatcggg gggttcagga attcaccgct gactgatctt gtctgcaatg
     1381 cagaaagcgg cacagtccag ttctctgtgg gactaaagag gatcggaact ccaaaaaaca
     1441 tatcattccg cgttgcatgt ggcgggtgga aggcacctga gggagactgg gacattgcag
     1501 gccccgtttc ctcaagtctt gtccagtcgg acactccggt tttcacaacc attttcgagg
     1561 acaatgaaga cccaagcttc ccggtcgaca tgaaaacaat agagtgtgca aacaaccctt
     1621 caaaaggtaa tgttctcttc aaggtcggat tttacaaaga cccgactgcc tccggaaacg
     1681 atgtccaggt ctcaatattc ataaattctg accaggacgc atcaacaggg atgtttccag
     1741 agaacggcag cccgggcgga gaagacttca tcgtcaacgt ttatatgaag ggtggaaatg
     1801 tcatctgcag gctcctctct gcacaaaacg gcccgatctc tgaagtggca gagctggaag
     1861 ggactgtatt tacagatggg aagctccttg tgcctgttcc tctggaaatg attggaaacc
     1921 caaaatcact atctttttat gtcgtctgca tgtcatcagt caacccgatg gcacaggaca
     1981 aaagtccatt tgacctggcg tttacaataa caggcgaaat ggtcgaaaaa acctgcccaa
     2041 caacagtcaa ggtgtcggtc aagccagggg attcggcggc cacagtcatg tggagcgagc
     2101 catcaaccca ggccaatggc tttcttgtat acaggattga gggcaacaat gcaccaaagc
     2161 tcctgacgcc catacctctc cccgcaaatg ccaggcagtt cgaagatacc ggcctgacaa
     2221 atggcatgtc gtattcttac tacgtcaggg tcacatgccc cgatggctcg cttgggccat
     2281 catcagcact ggcgacaaca gtgcccagaa aacaggaaca gccctaccca aaaattgaaa
     2341 tagcaccaac gtcaatcaac cttggttcca tgcaaaacac aaagggcgca tacgccacct
     2401 tcaaagtaaa gaattccggc cctggcgatt tctcttgtga aataaaaccc gaaccggact
     2461 accttgcagc tttgccagac aagctggtgc tccagcaggg aaaatctgca gacatcagaa
     2521 ttgaagtgac aaaagatctg gcaacaggtc agcattttgg caggataatg gtcaaatcgg
     2581 caatcgggac cctctacatt gaaatctcgc tgaatgttcc agagatcggg ccacaattca
     2641 agtttgtgtc taatctgatt gcagagccaa tgccttttgc aatcaaactt tcatggctac
     2701 ctccaacata caggggcgag gaactcaagg gctacaggat tgaaaggact gagtattaca
     2761 gggggaaact cctgccagag aaaaaaatct ttgagtctcc gtcagggaaa aacgaatttc
     2821 tggacagtga ccttgaccca cagtctgtct acaactacaa agtggtcccg gtctggccat
     2881 ctggtgatgg aatccccaga atggctgaat catcaccttc tgccccgcaa gtggtcatca
     2941 tgatgaaggt gggaataaaa acggccgttg taaacgacag cacagtggaa ctggatgcac
     3001 caccctacat cacaaaggga aagacgatgg tgcctttcag gtttatcggg gaaaacctct
     3061 ttgcaaaggt cgattacgac gactacacaa aaacagtcac attcaaaagg ggcaggacaa
     3121 ccgtcaggat aaagattggg gagtcaaaag ctgaggtgaa cgggacactc atgacaatgg
     3181 atccgccggc aacaataaag gatggccgaa catttattcc ggtgaggttt gtctccgacg
     3241 cactcggtgc aaagacgctc tgggaggcct ctgccaggca ggttgtgata acgtacccgt
     3301 aggaaaaatg gtcaaaatgt agacattttg tttatatttc tggaaaatgc tttctataaa
     3361 atgtgtcatt gggaatacct gcccaaaagg aggaaaatat gttggcatgg ggtggaaaga
     3421 ttaaagtatt atccttgctt gttttgacag tcgtcactgc ctgttttctc tgtccaggca
     3481 caaaagcttc ttctatgaat atttctcttg gtgatgaatt tacaatagcg cggagctcgt
     3541 cagatgagta ttcgccattt atcaattcag attttgcaat tttcagtgaa aaaagtggcg
     3601 gaatcaccag cgcaaaatca ttcaatctcc ctgatggatc gctctcagca atatttgatg
     3661 ataagaatgg cgcgccttca tttccatccg acgagttgtg ctatatcggc caggattatt
     3721 gcgtaacttt gaaaaattca aattgctcct ccagctccag ctggatttcc gcatataatt
     3781 tcaagagcaa gaccacaact accatctcgg acaccccagc aaccagagat tgtgtttcaa
     3841 tttgggacaa atatgctgta tggactgaaa cagatgtttc aaatcctgga atctatatcg
     3901 ccgatattac aaacccatca ggcaaaacac gcatatacga ctttattgtc aacgatcagt
     3961 ggtgctggcc cgtggtttat gatggcaagg tggcatacga gtcttttgat ggcgcagatt
     4021 cagacataaa gttgtatgac ataaaaacaa aaataaccta cagtattgga gatcctgcgg
     4081 tttttgagaa aaatcctgtg atacagggtg actatgtata ctatacctcc tgttcactaa
     4141 gtctggactc ttttggaaaa ataaaaggcc acaagatctc gactggtgaa acttttgatg
     4201 tctggaacaa tcctgatgat tatttcctca cgcttatgtt tagccctgag tcagagattc
     4261 ttgtcgtgaa agtctttaat tttgacaacg gcagagaaaa gctaatgggt tttgaaccag
     4321 taaaatcaat gctttttgac atcactccgt ggcttcagga agattgcatc agcattgtaa
     4381 gcgagtgcgg ttttggaagg aaaattgtat ggattgagaa tcgtcgctcg gaaaatgaac
     4441 aatggtacaa aacacccggt gtaagattgg cgatcatgga cagcaatggc aacctgaaaa
     4501 tttttgacat cacaggccaa aat
//
LOCUS       DAOSRI010000141         4510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17442_length_4510_cov_3.769921, whole genome shotgun sequence.
ACCESSION   DAOSRI010000141 DAOSRI010000000
VERSION     DAOSRI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17442_length_4510_cov_3.769921"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PK717_05085"
     CDS             <1..883
                     /locus_tag="PK717_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47070.1"
                     /translation="ALVFNDFVRIYFSTRERDLTGKYLSHVSFIDMSNDFKKIINIAT
                     NTVIELGALGCFDEHGIFPFNVLRDKGRILAYTTGWTRRVSVSVDSGIGLAISKDNGL
                     TFQKIGQGPILSSSLHEPFLVGDAFVALFGDMYHMWYIHGTKWVRFSGNEAPDRIYKI
                     AHAISLDGISWQKEGRQIISDKLAPDECQALPTVIFFNNRYHMFFCYRQPDGFRRNKH
                     RGYRIGYAFSNDLKNWIRKDHEAGIDISKDSWDSDMICYPNVFHCDDKIYILYNGNEF
                     GRYGFGIAVLESDTSIT"
     gene            922..1482
                     /locus_tag="PK717_05090"
     CDS             922..1482
                     /locus_tag="PK717_05090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOR47071.1"
                     /translation="MNIKGNKVVLRAIEDSDLELLHKWANDPETQDVIGNVHFPSSMD
                     FHKMWFQSLKNDQLNLRLAIEIPGAGIIGISSIINIDWRNNHAWHGVMLGDPDVRGKG
                     YGVDAVMATMRYAFEELHLERLDGSIIDYNKISYAFYCGKKLGWKEEGRRRNYYFRKG
                     RYWDQIVVGVTKQDYENLIQNTGYWD"
     gene            1488..2231
                     /locus_tag="PK717_05095"
     CDS             1488..2231
                     /locus_tag="PK717_05095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR47072.1"
                     /translation="MGRNRDYNSELQDTKGHNYAYSFDYDVMHSYMIRSFEPLFNKGS
                     LLELGCFKGDFTRRLQSYFDDITCIEVSDVAIEDARKKLGNKVNFVHALFEKATLPKR
                     YDNIVMTHVLEHLDDPLLVLKRINNEWLAEGGRLFLVCPNANAPSRQIAVKMGLISHN
                     AAVTPSEAEHGHRRTYTLDTLERDAVAAGLKVAHRTGIFFKALANFQWDHLLQTDIIS
                     KEYLEGCYKLGQQYPDLCSSIFLLCEKGE"
     gene            2233..3159
                     /locus_tag="PK717_05100"
     CDS             2233..3159
                     /locus_tag="PK717_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR47073.1"
                     /translation="METTKLISLCFAVYNNESALTVLYKKMVTELETHFPKYDFELIF
                     VNDGSKDNSLAELLELKKQHNDNRIKIISLSRNFGQMAAILAGWHYAKGDAVINMAAD
                     LQDPPEQCVAMIKEWEKGSDVVISYRKSHGTALLNKVTSRLAYKMLLPNAPPGGFDFA
                     LLSRNAMDAVNSIKERNKFYQYDILWVGFNVKFIPYDKLERPIGKSQYNFVKRFGNFM
                     VGFINVSYLPLRIMSFLGFSFALAGFIYTLSILHAYYVKATPFQGWAPIMMLLLIIGG
                     LIMIMLGILGEYIWRIFDEVKNRPNYIIKDIQ"
     gene            3422..4459
                     /locus_tag="PK717_05105"
     CDS             3422..4459
                     /locus_tag="PK717_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011841288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HOR47074.1"
                     /translation="MTAEKKIAQKRLTLLQVAERIRNVSEACRRHSVSRSQFYEYKRA
                     FQERGFDGLMDRPPIPKSFPNETPVDVKEKIIALSLKHPAWGQVRLSDHLRLEGILVS
                     PSTIRNIWIKEDLVTRYRRLLRLEEEKNGQDINLTEEQIRFLEKANPCFRERKVESPY
                     PGYLLSQDTFHVGRIKGVGRIWLQVVVDTYGSFAFGKLYTSKLPETAVDVLYDRVLPF
                     YESQELKIEHILTDNGREYCGRPMIHPYQIYLELNDIGHRRTKVARPRTNGFVERFNR
                     TVLDEFFRETFREKLYTTVEELQEDLDKWLHSYNYERPHRGYRNMGRRPIETIEAGKV
                     IKEQETPKQAA"
BASE COUNT         1345 a          867 c         1070 g         1228 t
ORIGIN      
        1 agccctcgtc ttcaatgact ttgtcaggat ttacttctcc acaagagaaa gagacttgac
       61 aggaaaatat ttgagccatg tttcttttat agatatgagc aatgatttca agaaaataat
      121 caatatcgct accaatacag tcattgaatt gggcgccttg ggttgcttcg atgaacacgg
      181 tattttccct ttcaatgtcc ttagggataa gggcagaatt cttgcctaca caacagggtg
      241 gaccagaagg gtttctgttt cggttgactc aggtattggc ttggcaatta gcaaagataa
      301 cggtcttacc tttcaaaaaa tcggacaagg tccgatacta tcgtcgtcgc tgcatgagcc
      361 ttttcttgtt ggtgatgcct ttgttgctct cttcggcgat atgtatcata tgtggtatat
      421 acacggtacg aaatgggtaa ggttcagcgg caatgaagct cctgaccgga tttacaaaat
      481 tgctcatgca atttcacttg acggaatttc ctggcagaaa gaaggcaggc agatcataag
      541 cgacaagctt gcccctgatg aatgccaggc actacctacg gtcatttttt tcaataatcg
      601 ataccacatg ttcttttgtt acagacagcc ggatggcttc agaaggaaca aacacagagg
      661 ctaccggatc ggatatgcct tttcaaatga tttgaagaat tggataagaa aagatcatga
      721 agcgggtatt gacatttcta aagacagttg ggattcggat atgatatgtt accccaatgt
      781 gttccattgt gatgataaaa tctatatatt gtataatggc aacgaatttg gacgttatgg
      841 ttttggtatc gctgttctag aaagcgatac aagcataaca taaatccaaa atcaacagaa
      901 cagttaaatc atgggggggc aatgaatata aaagggaata aagtagtgct cagggctatc
      961 gaagatagcg atctcgaact actccacaaa tgggcaaatg atccagaaac acaggatgtc
     1021 attgggaatg tgcatttccc aagctctatg gattttcaca agatgtggtt tcaaagtcta
     1081 aaaaatgatc aattaaatct taggcttgct atagagatac caggtgcagg tatcattgga
     1141 atatccagta tcatcaacat tgattggcga aacaatcatg catggcatgg tgtcatgttg
     1201 ggcgatcctg atgttcgggg aaagggctac ggtgttgatg cagttatggc aacaatgaga
     1261 tatgcatttg aagaattgca tctcgaaagg cttgatggtt caattattga ttacaataag
     1321 atatcttatg cattctattg cggaaaaaag ttaggttgga aagaagaagg aagaaggaga
     1381 aattattact tcaggaaggg acgctattgg gatcagatag tcgtcggtgt tacaaaacag
     1441 gactatgaga atctaataca aaatacagga tattgggatt gaaacatatg ggtagaaaca
     1501 gagactataa tagtgaattg caagatacaa aaggtcataa ctatgcgtat agctttgatt
     1561 acgacgtaat gcactcttac atgatcaggt cgtttgagcc cttatttaac aagggaagtt
     1621 tgcttgagct gggatgcttc aagggcgatt ttactagaag attacaatca tatttcgatg
     1681 atatcacctg tattgaagtc tccgacgtgg ctattgagga tgccagaaag aagctgggca
     1741 acaaggttaa tttcgtccat gcattattcg agaaagcgac cttgcccaag cgctatgaca
     1801 acattgtcat gacccatgta ttggaacatc tggatgatcc attgctggtt ttaaaacgca
     1861 tcaataatga gtggctggcc gaaggcggca ggttattttt ggtgtgcccg aatgccaatg
     1921 ccccttcgag gcagatcgcg gtcaaaatgg gattgatctc tcataatgcc gccgtgacac
     1981 catcggaagc cgagcatggt caccgacgca cctacacatt ggatactctg gaacgcgatg
     2041 ctgtggctgc tgggttgaaa gtggctcatc gaacaggtat tttcttcaag gcgttggcga
     2101 atttccagtg ggatcacttg ttgcaaacgg atatcatctc caaagagtac ctggagggat
     2161 gctacaaact tgggcagcaa tatccggatt tgtgttccag tatattccta ttgtgtgaaa
     2221 agggggaata atttggaaac aacaaaatta atatcccttt gttttgcggt ttataataat
     2281 gagagtgctt tgactgtcct ctacaagaaa atggttacag aacttgagac ccattttccc
     2341 aaatacgatt tcgaattaat atttgtaaac gacggatcaa aagataactc actcgcggaa
     2401 ttattggaat taaaaaaaca gcacaacgat aatagaatta aaatcatttc tttatccagg
     2461 aattttggcc aaatggccgc catattggcg ggttggcatt atgccaaagg agatgcggtg
     2521 ataaatatgg cggctgattt gcaggatcca cctgagcaat gtgttgccat gataaaagaa
     2581 tgggaaaaag gcagtgatgt tgtgatatct tatagaaaga gtcatggaac agcgctacta
     2641 aataaagtta cttcaagatt agcttataag atgctcctgc caaatgcacc tccagggggt
     2701 tttgattttg cgctcttaag tagaaatgcc atggatgcag tcaacagcat taaagagaga
     2761 aacaaatttt accagtatga tattctatgg gttggtttta atgtaaagtt tattccatac
     2821 gataaattgg aaagacccat tggtaaatct caatacaatt ttgttaagcg ctttggtaat
     2881 tttatggttg gtttcattaa tgtgagttat cttcccttaa ggattatgtc ttttctggga
     2941 ttttcttttg cgctggccgg ttttatatat acattaagca tattgcatgc ttattatgtc
     3001 aaagccaccc catttcaagg gtgggcgcca atcatgatgc ttcttctaat tattggggga
     3061 ttaatcatga taatgctggg gatattaggt gaatacatct ggaggatttt tgatgaagta
     3121 aaaaacaggc ccaactatat tattaaagac attcaatgat aatcgctctt catcaaagcg
     3181 agatgtaccc atatgaattc tcctgacgga aaagacaagt atgtacacgg catggattgg
     3241 ggtgctggac tggaaagttt taagctttct aattatcgta aatatcagtt taaccttata
     3301 aacaagtata tagtgtaaaa gttaagatct ccccgtacag ttaggggaat aaaaagtggc
     3361 agtcagtctt ctcttcggtt agagtgtttg taccaccaaa tactttacca aggaggaaga
     3421 tatgaccgcc gagaagaaga tagcacaaaa gagacttaca ttgctacaag ttgctgaaag
     3481 aatccggaac gtatcggaag cctgccgacg ccacagcgtg tcccggagcc agttttacga
     3541 gtacaagcgg gctttccagg agaggggatt tgacggtttg atggaccggc cgcccattcc
     3601 caagagtttc cccaatgaga cgcctgttga tgtgaaggag aaaatcattg ccctgtcttt
     3661 gaagcatcct gcctggggtc aggtccgttt atccgatcat cttcgtttgg agggcatcct
     3721 tgtgagtccg agcacgatcc gcaacatctg gatcaaagaa gatctggtga cccgttatcg
     3781 acggctactg cgtctggaag aggagaagaa cggccaggac atcaatctca ccgaagagca
     3841 gattcgcttt ctggagaagg ccaatccctg tttccgggaa cgcaaggtgg agagcccgta
     3901 tcctggatat ctgctttccc aggacacctt ccatgtcggc agaatcaaag gcgttggcag
     3961 aatctggctc caggttgttg ttgataccta tggttctttt gcctttggga agctctatac
     4021 ctccaagctc cccgagaccg ccgtggatgt cctttatgac cgggtcctgc cgttctatga
     4081 atcgcaggag ctcaaaatcg agcatatcct cacggataat ggccgggaat actgcggacg
     4141 cccaatgatc catccctatc agatctatct ggaattaaac gatatcggtc atcggagaac
     4201 caaagtggca aggcccagga ccaatggctt tgtggagcga ttcaatcgaa ccgttctcga
     4261 tgaattcttc cgggagacct tccgtgagaa actctatacc acagtcgaag agctccagga
     4321 ggacctggat aaatggctcc attcttacaa ttacgaaagg cctcacaggg ggtacagaaa
     4381 catgggcaga cggccgattg aaaccatcga agccggcaaa gttatcaaag agcaggagac
     4441 gccaaaacag gcagcttaaa catcagcacc ggagagaagc tgactgtccg gaaagaggtt
     4501 gacttttaca
//
LOCUS       DAOSRI010000142         4498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17506_length_4498_cov_4.280216, whole genome shotgun sequence.
ACCESSION   DAOSRI010000142 DAOSRI010000000
VERSION     DAOSRI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17506_length_4498_cov_4.280216"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PK717_05110"
     CDS             <1..713
                     /locus_tag="PK717_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HOR47075.1"
                     /translation="FQMATDNAVSKTVNFPNNATREDVKEVFVLAYKLGCKGVTIYRD
                     GSRSEQVLSIKEVNKEAKAENVETGPIAPRERPEMTKGVTFRIRTGCGNLFVTINHDE
                     KGICEVFATMGKSGGCAAAQSDGIARLISLALRAGVDTKAVIGQIRGLRCPSPTWGQN
                     GAVLSCPDAISQALERVLSWETLDFGGNSEPPKNGHSTSLSGNILGLNPQCPDCGAML
                     EFAEGCVICKSCGYSKCG"
     gene            822..3026
                     /locus_tag="PK717_05115"
     CDS             822..3026
                     /locus_tag="PK717_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOR47076.1"
                     /translation="MKKLLLVALAVIIATMPFFLHHIESGQTSFSPKKYVTENVIVIN
                     TDGLRFVDGLGSEGKYLSHIWNDIRPNGAIFTKMYNETCTYTAPGHAQCITGAWQYEP
                     NNGRIRPSTPTMFEYYRRQFDAPPEDVCILPGKGNCWHLNYSTFPGFGRNYETPFYDL
                     GYGDDEIASKLAELLLTTKPKLVYAVLPHVDAVGHSGDYKKYTESISHADELIWKIWQ
                     QIQFNPNYKDKTTLIITTDHGRHTEGVRDGFKSHGDSCEGCRHTYCVMIGPDVKKGFV
                     YQYECLQVDIVPTVGELLGFATPACDGSSLMKALVDRPLKEIPDEQKAYILSLETECR
                     RFKQSKYDQDLPRMLDASLKIPAASLGHSINDAIFLKGVLESSVFLKNSSGIDYVKAW
                     ADSWIAKDVPRDDITDSLCGEVLFELFDATNDSKYKDMARGIADWVMSKPFGVSEKGA
                     LLCKEIKSPKLQGTAKLIKADTLYAVVPLLCYAQKLFDGEYAEFALGQYEAHKKHLAD
                     EKGLFRHFTLANASLKINPINWVRGNAFVLGSILEASSILGEDKDFYKKISELITKEP
                     LGDQIVMRQQQNGLWMDDLSGQVSDYDTVANSILIGAISKWQGEKVSRRVGEGKLSEM
                     YFDEDAPATMAVLPDKKKVTKESALNGSMYHVIGAWDSMWPQISESGLVYGCAEPAYD
                     AQLYYQGFIPPVYSRDRGFYKEGQGAFMSALVGLSSLCSQLGLVNKRIPPIK"
     gene            complement(3089..3526)
                     /locus_tag="PK717_05120"
     CDS             complement(3089..3526)
                     /locus_tag="PK717_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOR47077.1"
                     /translation="MIWKLNGDFLAIRLDPGDDLLECVGEAVNSSGIGSGFITSCVGA
                     LKKARFTSILSTPEGFKYAPMREVEGAVELLLASGNIEPKEAGGTKVHIHAMLCIDDH
                     EFTGGHLDDHGNIIGVTAEILVAKFPGIIRETPKGPSAPIKFN"
     gene            complement(3528..4151)
                     /locus_tag="PK717_05125"
     CDS             complement(3528..4151)
                     /locus_tag="PK717_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOR47078.1"
                     /translation="MQRVSLKAEERGDTKGHNAQLRAQGTIPAVIYGKGMQGSKPVQV
                     NEKEFVLLSRKGLSTLLFDLDINGTKRTALVKECQMHVITRRVQHIDFYTVKLDEPIE
                     VTLPVRLIGDAKGVKVGGRLEQRLYKIKVQALPEKIPNEIVVDVAEFDLGTLLHVRSL
                     AAPEGIAFTTSPDAMILTIKAPKAAAEAETPAAGETAATPPAATPQQ"
     gene            complement(4165..>4498)
                     /locus_tag="PK717_05130"
     CDS             complement(4165..>4498)
                     /locus_tag="PK717_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47079.1"
                     /translation="DVIFVDDIVQTGGTMVTAAYEAHKNGAKRIFACATHGLLGGNAV
                     EKINQSPIIELVITNTIAIPQEKRAGKIKVLSVAPLIAEAIIRIRDHRSISELFNVPV
                     SERRIPGL"
BASE COUNT         1153 a         1001 c         1108 g         1236 t
ORIGIN      
        1 ctttccagat ggcaacagac aatgcagttt caaagacagt caacttcccc aacaatgcca
       61 ccagggaaga tgtaaaagaa gtttttgtcc ttgcatacaa acttggctgc aagggagtaa
      121 ctatatatcg cgatggttca agatcggaac aggtcttgag catcaaggaa gtaaacaagg
      181 aagcaaaggc cgaaaatgtt gaaacagggc caattgctcc aagggaacgc cctgaaatga
      241 cgaagggtgt caccttcaga ataagaactg gttgcggaaa tctttttgta acaataaatc
      301 atgatgaaaa gggaatttgt gaagtatttg caacgatggg caagtctggt ggttgtgccg
      361 cggcgcagtc agacggaata gcaaggctca tttcgcttgc cctgagggca ggtgttgata
      421 cgaaagcagt catcggacag ataaggggac tcaggtgccc atctccaact tggggccaga
      481 atggtgccgt tctttcctgt ccagatgcca tctcacaggc ccttgagcgg gttttgtcct
      541 gggagacact tgattttgga ggcaattcag aacccccaaa aaatggccac tccacttcgc
      601 tttcgggcaa tattcttggc ttaaatcctc aatgtcctga ttgcggggcc atgcttgagt
      661 ttgccgaagg gtgcgtgatc tgcaagtctt gcggatacag caagtgtgga tgatcgctct
      721 tgccactaat tttctaaaag cccactttgc tggtgggctt tttttgtttc tacagacaat
      781 tacgttatga ctctcgtaac agatggaaaa ggaggctgca aatgaaaaaa ctattgcttg
      841 tagcgcttgc agttattatt gccacaatgc catttttcct ccaccacata gaatctggac
      901 agacatcttt tagccccaaa aagtatgtca cagaaaacgt gattgtcatc aacactgatg
      961 ggttgaggtt tgtggacgga cttggttcag agggcaagta cttaagccat atctggaatg
     1021 atatcaggcc aaatggtgcc atcttcacca agatgtacaa tgaaacctgc acttatactg
     1081 cccctgggca tgcacagtgc atcaccggtg catggcaata tgagccgaac aatggcagga
     1141 taaggccctc aacgccaacg atgttcgagt attacaggcg ccagtttgat gcgccaccgg
     1201 aggatgtttg catcctgccc ggtaaaggta actgctggca cctgaactat tcaacattcc
     1261 caggttttgg aagaaactat gagaccccat tttatgatct tggctatggc gatgatgaga
     1321 ttgcctcgaa acttgcggag cttcttctga ccaccaagcc aaagcttgtt tacgcagtat
     1381 taccccatgt cgatgctgta ggacactcag gtgattacaa gaagtacaca gaatccattt
     1441 ctcatgccga tgagctcata tggaaaatat ggcagcagat acagtttaat ccaaactaca
     1501 aagacaaaac aaccctcata ataactaccg atcatggcag gcataccgag ggtgttaggg
     1561 atggttttaa atcgcatggc gattcgtgtg agggttgcag gcacacctat tgcgtaatga
     1621 tcggccctga tgtcaaaaag ggttttgttt atcagtatga atgcctccag gtcgacatcg
     1681 tcccgacggt tggggaactg cttggatttg ctacgcctgc ctgcgatggg agttctttga
     1741 tgaaggccct tgtagacagg ccgctaaaag aaattcctga cgagcagaag gcatacatcc
     1801 tttcccttga gactgaatgc aggagattta agcagagcaa atatgatcaa gaccttccac
     1861 gcatgcttga cgcaagcctt aaaatcccgg ctgccagttt gggacattca ataaacgatg
     1921 ccatattctt gaagggtgtt cttgagtcct cggtgttttt gaaaaacagc agtggcatag
     1981 attacgtgaa ggcttgggct gattcgtgga tagcaaagga cgtgccaaga gacgacatta
     2041 ctgattcatt atgtggcgaa gttctctttg agctttttga tgccacaaat gattccaagt
     2101 acaaagacat ggcaagggga attgctgact gggtaatgtc caaaccgttt ggcgtgtccg
     2161 agaagggtgc actcctgtgc aaagaaatta aatcgccaaa gctccagggt acagccaaac
     2221 tcatcaaagc ggacacgctt tacgctgtgg tccctctttt gtgctatgcc cagaagctgt
     2281 ttgacggcga gtacgcggaa tttgcgcttg gccagtatga ggcacacaag aaacatctgg
     2341 ccgatgaaaa gggccttttc aggcatttta cactggcaaa tgcttcactc aaaattaatc
     2401 caataaactg ggtaaggggc aatgcttttg ttttgggctc aatccttgag gcctcatcaa
     2461 tacttggtga agacaaagac ttctacaaaa agatttcaga attgatcaca aaggaacctt
     2521 tgggtgacca gatagtcatg aggcagcagc aaaatggttt gtggatggac gacctctcag
     2581 ggcaggtatc tgattatgat actgttgcaa attccatatt gatcggtgcc atttcaaaat
     2641 ggcagggtga aaaagtatcc agaagagttg gcgagggcaa gctgtcagag atgtattttg
     2701 acgaagatgc acctgccaca atggctgttt tgccggacaa gaagaaggtt acaaaggaaa
     2761 gcgcgctcaa tggctcgatg taccatgtaa taggcgcctg ggactcgatg tggccacaaa
     2821 tatcagaatc tggtctggtg tatggttgtg ctgagccggc ttatgatgcc cagttgtact
     2881 accagggttt tattcctcca gtttacagca gggacagggg cttctacaaa gagggccagg
     2941 gtgctttcat gtccgcactt gtaggtcttt ccagcctgtg ttcccaactt gggctagtaa
     3001 acaagagaat tccgccgata aaataggtaa acaaacaaaa aaggcgctcc ttgtaggcgc
     3061 cttttttgtt tgttttggag atgggtcttt agttgaattt tattggcgca gaaggccctt
     3121 ttggagtttc cctgataata cctgggaact ttgcgaccag tatttcagcg gtaacaccaa
     3181 tgatgttgcc atgatcgtcc agatgacctc cagtgaactc atggtcatct atgcaaagca
     3241 ttgcatggat gtggaccttt gttccgccag cctcttttgg ctcgatgttt cctgatgcaa
     3301 gaaggagctc gactgctccc tcaacttccc tcatcggggc gtacttgaag ccctcgggag
     3361 ttgaaaggat cgaagtgaat ctggcttttt tcaatgctcc aacacatgaa gttataaaac
     3421 ctgaaccaat accagaagag tttacagctt cccctacaca ttcaagcaaa tcatcgcctg
     3481 gatcaagtct gatggccaga aaatctccgt tcagtttcca gatcatctca ctgttgagga
     3541 gtagcggccg gtggtgttgc tgctgtttcg ccagcagctg gtgtttctgc ttctgccgcg
     3601 gcttttggtg ccttgattgt taggatcatt gcatctggcg aggtggtaaa ggctattcct
     3661 tcaggtgctg ctaggcttct gacgtgaagc agggtcccga ggtcgaattc tgctacatca
     3721 accacgattt cgtttggtat cttttctgga agtgcttgaa cttttatctt gtaaagtctc
     3781 tgctcaagtc ttccgccgac ttttacaccc tttgcatcgc caatgagtct tactggcagt
     3841 gtgacttcga ttggttcgtc gagttttacg gtgtagaagt caatgtgttg gacccttctt
     3901 gtgattacgt gcatctggca ttccttcaca agggcggtcc tctttgtgcc gtttatgtct
     3961 aggtcaaaca aaagcgtcga aagccctttc ctcgagagaa gaacaaactc tttctcgttt
     4021 acctggacgg gcttggagcc ttgcatgcct ttaccataaa tcacggctgg gatggtcccc
     4081 tgggccctga gttgtgcatt gtggcccttg gtgtctcctc tttcctcagc tttaagtgat
     4141 actctctgca tgtcttactc cttcttatag tcctgggatt cttcgttcgc tcaccggaac
     4201 attgaacagt tccgagatcg aacgatggtc tcttatacgg ataattgcct cggcaatcaa
     4261 gggtgcaacg gagaggacct taatttttcc tgcccgtttt tcttgtggaa ttgctatagt
     4321 gttggttatt acaagttcta ttattggtga ttggtttatt ttttctacgg cattaccacc
     4381 aagaaggcca tgtgttgcac aggcaaagat cctttttgca ccatttttat gggcttcgta
     4441 tgctgccgtt accatggtgc cgccagtctg gacaatgtca tcaacaaata ttacatct
//
LOCUS       DAOSRI010000143         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_17779_length_4440_cov_4.094869, whole genome shotgun sequence.
ACCESSION   DAOSRI010000143 DAOSRI010000000
VERSION     DAOSRI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17779_length_4440_cov_4.094869"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="argS"
                     /locus_tag="PK717_05135"
     CDS             complement(<1..842)
                     /gene="argS"
                     /locus_tag="PK717_05135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOR47080.1"
                     /translation="MSIKTHISKIFSDALEKMGVFDVEAGITRPDSKEHGDYSTNVAM
                     GLSKQLKKPPRAIAEEIVSLTDKSIFEKVEVVGPGFINIFLKKDVVSGFVKQALEDSS
                     YGKWCVGGGKKVQVEFVSANPTGPITVANGRGGPLGDSLARLLEWTGYDVHREFYVND
                     RGAKVVHLAGSLEARYKQALGMPLVLPPEGYPGEYLLDIAKNIAKEFGEEPLGWDEAR
                     RLEFFGKQAVERIVATQKAVFEKFGIKFDRWFSEREMAERGEIDQTLSMLSEEGVLYQ
                     QDGAT"
     gene            complement(839..1747)
                     /locus_tag="PK717_05140"
     CDS             complement(839..1747)
                     /locus_tag="PK717_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOR47081.1"
                     /translation="MEKKFTRGSPNADKVEATKTAQSLLKKLNTLLEKRKIVDYSVKL
                     QKNKVLLVLPTMLEEGKREKLVQAIMELQGSDNIKIQDNKGPARLLSDEDIEDQFRIS
                     LQKYIPKETKVSGSVRDGVMTVYGTTTSAYAREAILDLAKETPGVKKVVDQTRLPMAK
                     SDIDLANSVMLELGNEAGISVYHLQVMARNGNVFVTGNAKDDKSVKLATDVISKVSGV
                     KSVENAIRIPGETITTDDKLRDKIFDSLIRSSSVRARDIKVAVVHGNVFLRGYAASTK
                     EIIAAESMVSSMPGVKKVYMELEPRL"
     gene            complement(1761..2405)
                     /gene="purQ"
                     /locus_tag="PK717_05145"
     CDS             complement(1761..2405)
                     /gene="purQ"
                     /locus_tag="PK717_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOR47082.1"
                     /translation="MKVGVVIFPGSNCDMDALWTTGVVKHHESFPIWHQSQDLNGAEL
                     IILPGGFSYGDYLRTGAIAKFSPVMEKVIAHANSGGLVLGICNGFQILTESGLLPGAL
                     LRNDTLKFESRWVELSVETTRTKFTKNLSKGDVLRVPIAHGDGRYYIDNIGLDSLISH
                     DQIVFRYKGKNPNGSVHAIAGICNRDGNVLGMMPHPERAGEDVLGSSDGLKILL"
     gene            complement(2402..2644)
                     /gene="purS"
                     /locus_tag="PK717_05150"
     CDS             complement(2402..2644)
                     /gene="purS"
                     /locus_tag="PK717_05150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOR47083.1"
                     /translation="MYKIEVLVHLKPDILDPQGKTVQNSLSKLGFKDVRSVRVGKSIE
                     LEIEAGSQDEAVKIATEMAGKLLSNPVMEVFAVRPK"
     gene            complement(2631..3332)
                     /locus_tag="PK717_05155"
     CDS             complement(2631..3332)
                     /locus_tag="PK717_05155"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR47084.1"
                     /translation="MEKLYEGKAKIVYKTDDPDVLVCEYKDSLTAFDGTKKDSMEEKG
                     ELNAKISSAIFSYLTKQGIPNHFIEFVPPRTQKVKKVEIIPVEVVIRNVMAGSISKRL
                     GIPEGTALSEPLIEFYLKNDDLHDPIICENHVTGLGFATRQQLDVIKGLAFKVNDAMK
                     KFFLDVGLRLIDFKLEFGTHKGQVILADEFSPDTCRLWDVNTNEKMDKDRFRRDMGGV
                     MEAYLEVARRIHVQD"
     gene            complement(3336..>4440)
                     /gene="purB"
                     /locus_tag="PK717_05160"
     CDS             complement(3336..>4440)
                     /gene="purB"
                     /locus_tag="PK717_05160"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOR47085.1"
                     /translation="VTDHDVVAFVNALGETVGEDSCYIHKGATSSDILDTATSIQIQE
                     AGKLIISEIDKLVSIVRKKAEEYKYVPMMGRTHGVHAEPITFGLKLLSWYQELKRDRD
                     RVAQAVEINSVGKMSGAVGTYANLHPDLEMLVCERLGLKPDPLSTQVIARDRHAEYLL
                     VLSMLGASLERFALQIRLLQRTETGELEEPFKAGQTGSSAMPHKKNPKTCEQVCGLAR
                     LLRGNAMVSLEDVALWDERDISHSSVERIILPDTSVLATYLVRRFARIVDGLFVNADR
                     MRKNIEATGGAIYSGRLLVSLMTRGMGRKEAYEIVQRLAIPASKGGTPLREACKADAQ
                     VASLLSAAEIDNIFDVGFYLRYVDDIFRRNLEE"
BASE COUNT         1033 a         1218 c          955 g         1234 t
ORIGIN      
        1 gttgcgccgt cctgctggta gagaacaccc tcttcagaaa gcattgaaag cgtctggtct
       61 atctccccac gctcagccat ctcacgctct gaaaaccacc tgtcaaactt tatcccgaac
      121 ttctcgaaaa cggccttctg tgtggcaaca atcctctcta cagcttgctt tccaaaaaat
      181 tccagcctcc ttgcctcatc ccagccgagt ggctcttctc caaactcttt tgcaatgttt
      241 tttgctatgt caagcagata ctcacccgga tagccttctg ggggaagaac aagcggcatg
      301 ccaagcgcct gtttgtatct ggcttccagt gatccggcta gatgcacaac cttggcgccc
      361 ctgtcattta catagaattc cctgtgaaca tcataacccg tccactcaag gagcctggca
      421 agcgaatcac caagaggccc accccttccg ttggcaacgg ttatcggccc tgtcggattt
      481 gcagaaacaa attccacctg cacttttttc ccaccaccaa cacaccactt gccataagaa
      541 ctgtcttcca gggcctgttt tacaaaacct gaaacaacat cttttttgag aaaaatattt
      601 atgaagcctg ggccgacaac ttcgaccttt tcaaatattg acttatcagt caatgaaaca
      661 atctcttccg caatcgccct tggcggcttt ttcaactgct ttgaaagccc catggcaaca
      721 ttggtggaat aatcaccgtg ttctttcgag tcgggacggg taatgcccgc ctcgacatca
      781 aagacgccca tcttttcgag ggcgtctgaa aatatctttg aaatatgggt ttttatgctc
      841 acaatcttgg ctccagctcc atgtagactt tttttacgcc aggcatcgaa gaaaccattg
      901 actctgcggc aataatttcc tttgttgagg cggcgtagcc cctcaaaaac acgttcccgt
      961 gaacaactgc cacttttatg tccctggccc taacggaaga cgaccttatc agggaatcaa
     1021 atatcttgtc cctcagcttg tcatctgtag taatcgtttc gccagggatc ctgatggcat
     1081 tctcgacaga tttcacccct gaaaccttgg agattacatc tgttgccagc tttacagact
     1141 tgtcgtcctt tgcgttgccc gtcacaaaaa cattcccgtt cctggccatc acctgaaggt
     1201 ggtaaaccga gatgccggcc tcattgccaa gctccagcat cacagaattg gccagatcaa
     1261 tgtccgattt agccatcggc aaccttgtct ggtcaactac cttcttcaca cctggcgttt
     1321 ctttcgccag atcaaggatg gcctctctgg cataggccga ggttgtggtg ccataaaccg
     1381 tcatgacacc atccctcaca gaaccactga cttttgtttc tttggggatg tatttctgaa
     1441 gggaaattct aaactggtct tcaatgtcct catcggagag caacctggca ggccctttgt
     1501 tgtcctgaat cttgatgttg tcggaaccct gaagctccat gatcgcctgt acaagctttt
     1561 ctctctttcc ttcttcaagc attgttggca aaacaagcaa aactttgttt ttttgaagtt
     1621 ttacggagta gtccacaatc tttctttttt ccaaaagggt gttcagcttt ttcaacagcg
     1681 actgtgcggt ctttgtcgct tccaccttgt cagcgtttgg tgaacctctg gtgaactttt
     1741 tctccaaaaa tgcctccttg tcaaagaagt atttttagcc cgtcggaaga gccgaggacg
     1801 tcttctcctg ccctttccgg gtggggcatc attcccaaaa cattgccatc cctgttgcag
     1861 attccggcta tggcatgcac agaaccatta gggtttttgc ctttgtatct gaaaacaatc
     1921 tggtcgtggg atatgagact atcaagaccg atattgtcaa tgtagtatct tccgtctcca
     1981 tgcgctatcg gcactctaag cacatcgccc tttgaaagat tttttgtgaa ttttgtgcgc
     2041 gtggtttcaa ccgagagttc gacccacctg gattcaaact tgagcgtgtc attgcgcaaa
     2101 agcgcacctg gcagcaggcc cgattcagtg aggatctgga aaccattgca aatcccaagc
     2161 acgaggccac cggaatttgc atgggcaatg accttttcca ttacagggga aaattttgct
     2221 atcgcaccag tgcgcaggta atcaccataa gagaaaccac cgggcagaat aatgagctca
     2281 gcaccattga ggtcctggga ctggtgccat atcggaaacg actcatggtg ctttacgaca
     2341 ccagttgtcc acaacgcgtc catatcgcag tttgagcctg ggaatattac aactcctact
     2401 ttcactttgg cctcaccgca aacacttcca tgaccgggtt cgaaaggagc tttcctgcca
     2461 tctctgttgc aatctttacg gcctcatcct ggctcccagc ctcaatctcc agctcaatgc
     2521 tcttgccaac ccttacagat ctcacgtcct tgaatccaag cttactgagc gaattttgca
     2581 cagttttgcc ctgcgggtcc agaatgtccg gcttgagatg gaccaaaacc tcaatcttgt
     2641 acatgtatcc tcctggcaac ttcaaggtac gcttccatga ctccgcccat gtctctcctg
     2701 aagcggtcct tgtccatttt ttcgtttgta ttcacatccc agagcctgca ggtgtccggt
     2761 gaaaattcat cggcaagaat aacctgccct ttgtgggtcc caaattcgag cttgaaatca
     2821 atcaggcgca aacccacgtc caggaaaaac tttttcattg catcatttac cttgaacgcc
     2881 aagcctttga tgacatcaag ctgctgcctt gttgcaaaac caagccctgt tacatggttt
     2941 tcgcagatga tcgggtcatg gaggtcatca tttttgaggt aaaattcaat aagcggctct
     3001 gaaagtgcag ttccttccgg aatgccaagc ctctttgaga ttgagcctgc cattacgttc
     3061 ctgattacaa cctcgaccgg tattatttcg acctttttga ccttttgcgt ccttggcgga
     3121 acaaactcga tgaaatggtt gggaattccc tgttttgtga ggtaagagaa gatggcagag
     3181 gaaattttgg cattgagctc gcccttttcc tccattgaat ctttttttgt tccatcaaat
     3241 gcggtcagcg aatctttgta ttcgcaaaca agcacatctg ggtcatcagt cttgtagact
     3301 attttcgcct tgccttcata gagtttttcc atgtatcact cctccaggtt tcttctgaat
     3361 atgtcatcaa cataacgcag gtagaaccca acgtcaaaga tattgtctat ttcagccgca
     3421 gaaagcaggg atgccacctg ggcatcagcc ttgcaagctt ccctcaatgg cgtcccgccc
     3481 ttggaggccg gaattgcgag cctctggact atctcgtagg cttctttcct gcccataccc
     3541 cttgtcatca gggacacaag gagccttccg ctgtaaatgg cgccaccggt ggcctcgatg
     3601 tttttgcgca tcctgtctgc attcacaaag agtccgtcaa ctattctggc aaacctcctg
     3661 acaagatatg ttgcaaggac agatgtatca ggaaggataa ttctttcaac agaggagtgt
     3721 gatatgtccc tctcgtccca gagcgcaaca tcctcaaggg aaaccatcgc gttgcccctc
     3781 aggagcctgg ccagtccgca aacctgctcg caggtttttg ggtttttctt gtgcggcatg
     3841 gctgatgaac ctgtctgccc ggccttgaag ggttcctcaa gctctccagt ttccgttctc
     3901 tggagcaacc tgatctggag tgcaaacctc tccagcgatg cgccaagcat ggagagcaca
     3961 agcagatact ctgcgtgcct gtccctggcg atgacctggg tcgaaagtgg gtcaggcttg
     4021 agcccaagcc tctcgcacac gagcatttca aggtctggat ggaggtttgc ataggtgcca
     4081 acggcaccgc tcatctttcc aacactattg atttcaactg cctgcgcaac cctgtcccta
     4141 tctcttttca gttcctgata ccaggaaagg agcttgagtc cgaaagttat tggctcggca
     4201 tgcacaccat gcgtgcggcc catcatcggg acgtatttgt attcctccgc ctttttgcgc
     4261 acaattgaaa caagcttgtc tatctcggaa attatgagct ttccggcttc ctgtatctgg
     4321 attgaggttg cagtatcaag aatgtccgat gatgtggccc ccttgtggat atagcatgaa
     4381 tcttcgccaa ccgtttcgcc aagggcattc acaaaagcca ccacatcgtg gtctgtcacc
//
LOCUS       DAOSRI010000144         4330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18328_length_4330_cov_3.622222, whole genome shotgun sequence.
ACCESSION   DAOSRI010000144 DAOSRI010000000
VERSION     DAOSRI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18328_length_4330_cov_3.622222"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PK717_05165"
     CDS             complement(<1..838)
                     /locus_tag="PK717_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47086.1"
                     /translation="MKKLFSVILVFAIVAPGIAFAAPNIPVKTDIPLGGLLIGNPSGF
                     SKRIDCSVEPDSISVFKGQLFATGLSRALEIGMPAVPSFAERIQVPSGSYPKGAYLKE
                     ISFTNIPLPKLEPYYPKTWGKELGIQKQTEPKGFFPKSLGDIKMENGNDGQVATVYFN
                     PIVLDFDKRTIRIAKKAIIDVVFESNTIYSSSSEKPQNKAVIVCPNLLIEAAKKLEQA
                     QKNDGYVVRTVTVEEIASTFEPQKEEPSYKGPKDFNEKDKKYLAKYDYELAKKIRNFC
                     AKE"
     gene            complement(857..3175)
                     /locus_tag="PK717_05170"
     CDS             complement(857..3175)
                     /locus_tag="PK717_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47087.1"
                     /translation="MKKCLSFLLALCFVFPVVSAQESAGTLIRGGIAADKLLFPTLAK
                     ADKEGNLYVYSKGTKELLSYNKDLKLRYRVGGFGGDAGYFADPSDLIVFKEDIVVSDV
                     GALLVFDLKGNFKKRVTKIGSVDLKRPLGLSLDYKNRLYISDPELGTVLICEDDFELW
                     RQIPKLDKPAQCYLMQNSNYLVLELGTKKLTILSSFYSKIKSFGEFTSPHSVWTDLSQ
                     KIVVLDDDSIKTFGMDSKLLEKTSFAPKHPSEGYCSFTPFEDGFVFASFLDHELVKIN
                     KTGKVSILISKDDSGLLVPSGLDVDSNGRIYVVDEAENIVRVMDQKGNPLFSIEAESP
                     KKIAIGGENLAIVNQAGVEVRTRAGEKLFLCKKEDVCDSVFGSEDSIFVLTDKGQVVR
                     FNGSTEIGIVASDMTSKSVAISAIEGHFAVATAGENRIIVYSQAGIKENVIDLAEEPS
                     DLIMLSPQRFIVLFQGKVELLDKSGGVMRSFGKSGGVRSFHKPTQEQIDYSLETDKFT
                     QPIAISKFGNWLYVLDKAAMRLVRFPKGLLLAPPVMKVTPELLDFGVVKPDTTTVREL
                     VIQNLGGESLSGSLVKFPKWVSVSPRIVNGDDVVIKITANTAHFIPKKTYLENIILET
                     NAGTIIVPCKLLTPEELPKQINIELKIGQKIAKVGGKTIDLGVAPFIDKGVTMVPLRF
                     LSEAFGGNVAFDEGFIEVSFDSRKIFASLQVGSNDVALEDGGETKLVKIMPPPAVKQG
                     KTFVPLKFFTDILDCEVYWDSITKQLRIVYSP"
     gene            complement(3177..>4330)
                     /locus_tag="PK717_05175"
     CDS             complement(3177..>4330)
                     /locus_tag="PK717_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR47088.1"
                     /translation="PEGLIEIIKRMMAKDPDCRFQNPNELVQALDIVMKSYRMPRGER
                     IKSKKIPRSNPKIKRKVRNVAIGVISAVIITGLGYVFFMLSSPSPASAFVKSEPKGAS
                     VFLKGPDDDEFQKLSNTDPEIKDLYPGKYTLKLELDGYKTHEQQFRMESGKNLDLGII
                     KLAKQGLMKILTGKVDWGKVSEVPGAFSVSIANTGESEIEIERSFTGDWIIFDQEPFT
                     IQPNEQKQIRVAVNPEKVSPGKSYSATVTFKPKNSSSKEVLIPITMEYVQSQGRPSST
                     HNNPPPASNQTDTKPKPGNSGENTGNSGSTTPPPPVKTTATVYITSNVPGAFIYIDGS
                     AKGTTPSSVTLAPGTHKIEVRMARFKSYSETINVSAGDEKTVNAVLRNK"
BASE COUNT         1212 a          956 c          822 g         1340 t
ORIGIN      
        1 attcttttgc gcaaaaattc cttatctttt ttgccagctc ataatcatac tttgcaaggt
       61 attttttgtc cttttcattg aaatctttgg ggcctttgta ggatggttct tccttctgtg
      121 gctcaaacgt tgaagctatc tcttcaactg ttacagttct tacaacatag ccatcatttt
      181 tttgggcttg ttccagtttt ttggcggctt caatcaacaa atttgggcaa acaatgactg
      241 ctttgttctg aggtttttca gaggaagagg aataaatggt atttgattca aaaacaacgt
      301 caataatggc tttcttggct attctgatcg ttcttttatc aaaatccagt actatgggat
      361 tgaaataaac agtggcaacc tggccatcat tgccattctc catctttata tcgccaagtg
      421 attttgggaa aaaacccttt ggttctgttt gtttctggat tccaagttct ttgccccaag
      481 ttttcggata atatggctca agcttcggca atggaatatt ggtaaaagaa atttccttca
      541 ggtatgcccc ttttgggtaa gaaccacttg ggacctgtat tctctctgca aaagaaggaa
      601 ctgcaggcat tccaatctca agggctctcg aaaggcctgt tgcaaaaagt tgacccttaa
      661 atactgaaat agaatcgggt tctacagagc aatcaatccg cttggaaaat ccgcttggat
      721 ttccaatcaa aagaccgcca agagggatat cggttttaac tggtatattt ggagctgcaa
      781 aagcaatccc aggtgcgaca atagcaaaaa ctaagatcac tgaaaacaac tttttcatga
      841 ttcttcctcc ctaaaactaa ggcgaataga caatccttag ctgtttggtt attgaatccc
      901 agtaaacttc gcaatcaaga atatcagtga aaaattttag tgggacaaat gtttttccct
      961 gcttaactgc aggaggtggc attattttta caagttttgt ttcgcctccg tcctctagag
     1021 caacatcatt cgagcccacc tggagtgaag caaatatttt tctggaatca aaagatactt
     1081 cgatgaaacc ctcgtcaaaa gcaacatttc cgccaaaagc ttcagataaa aacctgagtg
     1141 gaaccatcgt aacaccttta tctatgaatg gcgctacacc gagatcaatg gtttttcctc
     1201 caacttttgc tatcttctgc ccaattttca attcaatgtt tatttgcttt ggtaattctt
     1261 ccggggttag aagcttgcaa ggaacaatta ttgtaccagc atttgtttca agtattatgt
     1321 tttcaagata ggtttttttt ggtataaaat gggcagtatt ggcggttatt ttgattacaa
     1381 catcatcacc atttacgatt cttggagaga cagataccca ctttggaaat ttcaccaaac
     1441 tgccactcaa agattctcca ccaagatttt gtatgacaag ctccctaact gtggttgtat
     1501 ctggttttac aacaccgaaa tcaagcaatt ccggtgtaac cttcataact ggcggggcaa
     1561 gcagaagacc tttggggaac ctgaccagcc tcattgccgc tttgtccaga acatacaacc
     1621 agttaccaaa tttggatatg gcaatcggct gcgtaaattt gtcagtttcc aatgaataat
     1681 caatctgttc ttgcgttggt ttgtggaatg atcttacgcc tccacttttg ccaaatgacc
     1741 gcattacacc accactcttg tccaataatt cgactttccc ctgaaaaagg acaataaatc
     1801 gttgagggga aagcattata agatctgatg gctcttcggc aagatctata acattttcct
     1861 tgataccagc ctgggaataa acaataatcc tgttctcacc agctgttgct acagcaaaat
     1921 ggccttctat tgcagaaatg gcaactgatt tcgatgtcat atcggaagct acaatgccaa
     1981 tttcagtcga cccattaaaa cgaaccacct ggcctttatc agttaagaca aaaatggaat
     2041 cctctgaccc aaaaaccgag tcgcaaacat cttctttttt gcacaaaaag agtttctcgc
     2101 cagcccttgt tctcacctca acaccggcct ggtttacgat tgccagattt tctccaccaa
     2161 tcgctatctt ttttggagat tcggcctcaa ttgagaaaag cggattaccc ttttgatcca
     2221 tcacccttac aatgttttca gcctcatcga caacatagat ccttccattg ctatcaacat
     2281 caagaccgga tggaacaagc aatccagaat catcttttga tataagaatg gaaacttttc
     2341 ctgttttgtt aattttcacg agctcgtggt caagaaatga ggcaaacaca aaaccatctt
     2401 caaatggtgt aaatgaacaa tatccctcgc taggatgttt tggtgcaaaa cttgtttttt
     2461 caagcaattt cgaatccatg ccaaaagtct ttattgagtc gtcatcgagg accactattt
     2521 tctgagaaag atctgtccac acagagtggg gggaagtaaa ttcaccaaaa ctcttaattt
     2581 ttgaatagaa ggacgataag atggtcagtt ttttggtgcc aagctcgagt acgagataat
     2641 tcgagttctg cataaggtag cactgggcag gtttgtcaag ttttggtatc tgccgccaga
     2701 gctcaaaatc atcttcacaa ataagcacag ttccaagttc gggatcagat atatacagtc
     2761 tgtttttgta gtctagggac aagcccaatg gtctcttgag gtcaacgcta ccaatttttg
     2821 tgaccctttt tttgaaattg cccttcaaat caaaaaccag taacgcacca acatcagaaa
     2881 caacaatatc ttccttgaac acaatgagat ctgagggatc tgcaaagtaa ccagcatcac
     2941 ccccaaaacc acctactctg tatctaagtt tcagatcttt attgtatgag agcagctctt
     3001 ttgtcccctt ggaataaaca taaagattgc cttccttatc agctttggca agcgttggaa
     3061 acaataattt gtctgcagca attccccctc ttatcagcgt acccgctgat tcctgagcgg
     3121 aaaccaccgg aaacacaaaa cacaaagcaa gcagaaatga aaggcatttt ttcaatctat
     3181 ttgttcctca gcactgcatt gacagttttt tcatcaccag cagaaacatt tattgtctca
     3241 gagtatgact taaacctggc catcctcact tcaatcttgt gtgtgcccgg cgcaagcgtc
     3301 acactagaag gtgtcgttcc ttttgctgag ccatcaatgt aaataaaggc ccctggcaca
     3361 ttcgatgtta tataaaccgt agcggtggtt tttacaggcg gaggtggcgt ggttgagcca
     3421 gaatttccag tattttctcc gctattacct ggcttcggtt ttgtatcagt ctgatttgaa
     3481 gcaggtggtg ggttattatg tgttgatgag ggacgtccct gggattgcac atattccata
     3541 gtaattggga ttaagacttc ctttgaggat gaattcttcg gtttgaaagt cactgtggca
     3601 gaatagctct tgccaggtga aaccttctcc gggttaacag ccactcttat ctgtttttgc
     3661 tcatttggtt gtatagtaaa tggctcctgg tcaaagatta tccagtcacc tgtaaaagat
     3721 ctttcaattt caatctctga ttcaccggta ttcgcaatgg aaacggaaaa ggcaccaggt
     3781 acttcagaaa ccttgcccca atcaacttta cctgtaagaa ttttcatcaa accctgtttt
     3841 gcaagtttta tgattccaag atcaagattt ttgcccgatt ccattctaaa ttgttgctca
     3901 tgggtcttat agccatcaag ctcaagtttc agggtgtatt tgcccggata aaggtctttt
     3961 atctcggggt ctgtattgga cagcttttga aattcatcat catccggccc ctttaaaaac
     4021 acagaagcac cctttggctc agattttaca aacgctgagg caggacttgg agaacttagc
     4081 ataaaaaaca cgtatccgag tcctgtaata attaccgcag atataacacc gattgctaca
     4141 ttcctgactt tcctcttgat ctttggattc gatcttggaa tctttttgct cttaattctt
     4201 tcgcctctcg gcatcctgta tgatttcata acgatgtcaa gtgcctgcac aagctcattg
     4261 gggttctgga acctgcaatc agggtccttg gccatcattc tcttgatgat ttctataaga
     4321 ccctcaggta
//
LOCUS       DAOSRI010000145         4326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18350_length_4326_cov_4.182393, whole genome shotgun sequence.
ACCESSION   DAOSRI010000145 DAOSRI010000000
VERSION     DAOSRI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18350_length_4326_cov_4.182393"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /gene="murD"
                     /locus_tag="PK717_05180"
     CDS             <1..604
                     /gene="murD"
                     /locus_tag="PK717_05180"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOR47089.1"
                     /translation="GKDIYIRGKKVSSLSDYHLVGDFNALNLMAASLAVSKFGIDPAV
                     ALSTAKDFVPLDHRLQFVGEWEGIKFINDSKSTKPEATILAVKSLPGPFVIILGGSEK
                     GSDFSGLADNFSEVRYAVLHGATAPKIEKALRDGGFDRIIMVKDQSEAIKKAIKVAKP
                     GDTVLLSPACASFDQFKDYEHRGRMFCQEVRELAQKGLRF"
     gene            579..1697
                     /locus_tag="PK717_05185"
     CDS             579..1697
                     /locus_tag="PK717_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOR47090.1"
                     /translation="MPKRAYVFDKGLLFSTLSLVGIGLLAVYSAGVGKAFVGEVPSPG
                     YLFFKQLVAFIIGFTAGFVAYRSSIDTIKLLAYPIYFLAIGLLVLLYVPGFGVTINYS
                     TRWIEIAGQSFQPSELAKVGLILSTASYLSAREPRKMSVKEVFKLFLMFGVPALLVLR
                     SPDLGSTIVILTIFITQYFLSGAPMWGLIFLGALFVLGVLYESLTRVAYWKDRITAWL
                     DPLNPNLSDNVSYQMVQSLIAIANGGFFGSGLFYGQQKFGRLPMVESDFVFSLVVEEL
                     GFVGAVFVIAMFALFVVRAIRIASRSKNLFSYLVALGIGLHIGLQGFINMAVATGLLP
                     VTGVTLPFLSAGGTSLIVNMIEVGILFAIDRNNRQELT"
     gene            1694..2749
                     /locus_tag="PK717_05190"
     CDS             1694..2749
                     /locus_tag="PK717_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOR47091.1"
                     /translation="MTKTVIIGAGGTGGHILPAVAIAKELRSMGFRVVLAGLGSELEN
                     AVSMEFERRSIPALSPSMNPAKFIKYLSILNKGIKVANELIDELKPSCVLGMGNFSSV
                     PLVIAASKKSIPVALHEQNAIGGRANRLLSRRTDLVMVGFEGTIGFPPGKVRVVGNPI
                     RWSSIEPKKPEAYKKLGLDPKKKTVVIFGGSQGAKILNDVFLSLGKEIVENNEIQWLL
                     ISGSKTYDETKKNFNSRSYKNAVLLEYLGEMDLVYSVADVIVSRAGAMTISEIECSGV
                     PAILVPRALSINNHQMLNAAYLSKKGNCTVLEETQVREKLEGAIFSMMAKQRNPRNCM
                     HQDAGKRIAEILLAISR"
     gene            2689..>4032
                     /gene="murC"
                     /locus_tag="PK717_05195"
     CDS             2689..>4032
                     /gene="murC"
                     /locus_tag="PK717_05195"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOR47092.1"
                     /translation="MHASGRWKTDCRDTAGDIKMRVPRKGSKIHLIGIGGAGMRGIAL
                     MLMQMGYVVTGSDIDCDRELVRDLGEKGTILFSGQRAENITDQKVVIYSSAIKESNPE
                     LERARQLDIKCIHRTDFFFEFGLTRKVLGVAGTHGKTTTTAMIAHVLDRMGQNISYYF
                     GSPIIGGEQARWSGSGNQMLVMETDESDKSFLKFNCDLAVVTNIDWDHMDAYDHNKKN
                     LLDAFESFVVQAKMRVLNADDENTFRMKLRDMDQVITFGRNSLSHVKGDLTNIFREGH
                     NLCSTTGVRIGGESVGRLKLKVPGDHNVSNALAALSAIVSLDFDPKLALPHFFTFPGT
                     QRRLELMGSVNSSPVYDDYAHHPTAIIAGLKALRSYYPDEKICLVMQPFRFARISYLM
                     DEYSACVSLADRTIVTQVFPQEENNLERSREFCSKLRVMNPSSQIAMAGTLELVPK"
     assembly_gap    4033..4042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4102..>4326
                     /locus_tag="PK717_05200"
     CDS             4102..>4326
                     /locus_tag="PK717_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HOR47093.1"
                     /translation="MIPTPLTGLKSAIRGEVLFGEPMAHHTSFAVGGPADAFAVPYDA
                     DDVLNIYDYSCKMHVPVLVIGGGTNLLVGDL"
BASE COUNT         1145 a          875 c         1050 g         1246 t
ORIGIN      
        1 tggcaaagac atatatataa gagggaaaaa ggtttcgtcc ctatcagatt accaccttgt
       61 tggagatttc aatgcactta atctgatggc ggcctcgctt gcagtatcga agtttggcat
      121 cgatcctgca gttgcgcttt ctacggcaaa agatttcgtt ccgcttgacc acaggctcca
      181 gtttgttggt gaatgggaag gtatcaaatt cattaacgat tcaaaatcaa caaagcctga
      241 agcaacaatt ctcgcggtta agtcactccc tgggcctttt gtcataatcc ttggcggttc
      301 tgaaaaaggt tccgattttt cagggcttgc cgataatttt agtgaagtta gatatgcagt
      361 tctacatggt gctactgccc caaaaattga aaaagcgctt agagatggcg gttttgacag
      421 aatcataatg gtgaaagacc aatcagaagc cataaaaaaa gctataaagg tcgctaaacc
      481 tggcgatacc gttcttctct cgccagcctg tgccagtttt gaccagttta aggattatga
      541 acaccggggc aggatgttct gccaggaggt gagagaactt gcccaaaagg gcctacgttt
      601 ttgacaaagg gcttcttttc tcaacacttt cactggtagg tatcggtctc ttggccgttt
      661 attccgctgg tgttggaaaa gcttttgtag gtgaagttcc atcacctggc tacctgtttt
      721 tcaaacaatt agtggctttt attataggct ttacggctgg ttttgtggca tacagatcaa
      781 gcattgatac gataaagctt cttgcttatc cgatttattt cctggctata ggccttcttg
      841 tgcttcttta tgtccctggt tttggagtaa caatcaacta ttcaaccaga tggatcgaga
      901 ttgctggtca atcttttcaa ccttcagagc ttgcgaaggt tggcttgata ctttctactg
      961 cgtcctacct ttcggcaaga gaaccaagaa agatgtctgt aaaggaagtt ttcaagcttt
     1021 ttctcatgtt tggtgtgcca gcccttcttg ttctcagaag ccccgacctt ggctcaacca
     1081 tcgttatttt gacaatattc atcacacaat attttttgag cggtgctccg atgtggggtc
     1141 tgattttcct tggtgcgctt tttgtacttg gtgttttata cgaaagtttg accagagttg
     1201 cctattggaa agataggata acggcttggc tggacccttt gaatcccaat ctttcggata
     1261 acgtatcata ccagatggtg caatccctga ttgccattgc caatggaggt ttttttggtt
     1321 ccgggctttt ctacggccag caaaaatttg ggcgattgcc gatggtagaa agtgattttg
     1381 tgttctctct tgttgtggag gaacttggtt ttgtgggtgc agtttttgtg atagccatgt
     1441 ttgcgctgtt tgttgtgcgt gcaatcagga ttgcttcaag atcaaaaaat ctattttcat
     1501 atcttgtggc ccttggtata ggcctacaca ttggtcttca ggggtttata aatatggctg
     1561 ttgcaactgg tcttctgccc gtaactggag ttacacttcc atttctctcg gccggaggca
     1621 cctcgctcat tgtcaacatg attgaggtgg gtatactgtt tgccatagac agaaacaatc
     1681 gccaggagct gacatgacca agactgtcat tataggggcc ggtggtactg gagggcatat
     1741 tttgccggca gtagcaattg caaaggaact caggagcatg gggttcaggg ttgtacttgc
     1801 tggattgggg tcagagcttg aaaatgctgt ttcaatggaa tttgagagaa gatcaattcc
     1861 tgctttgtct ccatcgatga accctgcgaa attcataaaa tatttgagta ttctcaataa
     1921 ggggattaag gttgcaaatg agctgattga tgaactaaaa ccatcatgcg ttcttggtat
     1981 gggcaatttt tcatccgttc cccttgttat tgctgcctct aaaaagagta ttcccgttgc
     2041 actccatgag caaaatgcta ttggcggaag ggccaacaga ctcctctcac gcagaactga
     2101 tcttgtaatg gttggttttg aaggcaccat cggtttcccg cccggaaaag tgagggttgt
     2161 tggaaacccc ataagatgga gtagtattga gccaaaaaaa cctgaagcat acaaaaaact
     2221 tgggcttgat ccaaagaaaa aaactgttgt catatttggt ggttcgcaag gtgcgaagat
     2281 actcaatgat gtttttttgt ctctcggcaa agagattgtt gaaaataatg aaatccagtg
     2341 gctgctgatt tcaggatcaa aaacctatga tgaaacaaaa aaaaatttta attccaggag
     2401 ctacaagaat gctgtgcttc tggaatatct tggggagatg gatttggttt attcggtagc
     2461 tgatgtgata gtatcaagag ctggcgcgat gactatctca gagatcgaat gttctggggt
     2521 cccagccatc cttgttccaa gagcgctttc cataaacaat caccagatgc tcaatgcagc
     2581 gtatctttca aagaagggca attgcactgt gctggaagaa acacaggtta gggaaaaact
     2641 tgaaggtgca attttttcaa tgatggccaa acagagaaac cctaggaatt gcatgcatca
     2701 ggacgctgga aaacggattg cagagatact gctggcgata tcaagatgag agtgccaaga
     2761 aaaggctcta aaatacattt gattggtatt ggtggtgccg gcatgagggg cattgccctg
     2821 atgctcatgc agatgggcta tgtcgtgact ggttctgata ttgactgcga tagggaactg
     2881 gtgcgtgatc ttggtgaaaa gggcactatt ttgttctctg gacaaagagc tgaaaatatc
     2941 acagaccaaa aggttgtcat ttattcaagc gcaattaagg aatccaaccc agagcttgag
     3001 cgtgcaaggc aacttgatat aaaatgcatc catagaaccg acttcttttt tgaatttggc
     3061 ctgaccagaa aagttcttgg tgttgcagga acccatggca agacaacaac gacagccatg
     3121 atagcccacg tcctggacag gatgggtcag aacatttcat attattttgg gtctccaatc
     3181 attggcggtg aacaggccag atggtcagga tcaggcaatc agatgcttgt gatggagacc
     3241 gacgaatctg acaagagttt tcttaagttc aattgcgatc tggccgtggt gacaaatatt
     3301 gattgggacc atatggatgc ctacgaccac aacaaaaaaa acctccttga tgctttcgag
     3361 agctttgttg ttcaagcaaa aatgagagtc ctgaatgctg atgacgagaa cactttcagg
     3421 atgaagctaa gagatatgga ccaggtcatt acttttggga gaaatagtct gtcacatgta
     3481 aaaggcgatt tgaccaatat ttttagggaa ggccataacc tctgctcaac aacgggggtg
     3541 agaatcgggg gagagtcagt tggccgcctt aagctaaaag tgcctggtga ccacaatgtt
     3601 tcaaatgctc ttgctgcatt gtcggcaatc gtttcccttg attttgatcc aaaattggcc
     3661 ctgccgcatt tttttacatt ccccggaaca cagcgcagac tagaacttat gggatctgta
     3721 aacagcagcc ctgtatacga tgattatgcc catcacccca ctgctattat cgcagggtta
     3781 aaagccctca gatcatacta tccagatgaa aaaatatgtc tcgtgatgca acctttcagg
     3841 tttgcacgga tatcgtatct tatggacgaa tactcggcat gtgtttcgct tgcagacagg
     3901 acaatcgtaa cacaggtttt cccgcaagag gagaacaacc ttgaaaggtc gagggaattt
     3961 tgttccaagc tcagggtgat gaacccgagt tctcagattg ctatggctgg cacccttgaa
     4021 cttgtaccca agnnnnnnnn nntatcttgc aggcccttat cccataaggg cctgtgcgaa
     4081 accaatattg gaggcaatta gatgattcca acccccttga cggggcttaa atctgcaata
     4141 aggggggaag tgctcttcgg ggagccgatg gcccatcaca cgtcatttgc tgttggcggc
     4201 ccggcagacg cttttgctgt gccatatgat gctgatgacg tgcttaacat ctatgattat
     4261 tcatgcaaaa tgcatgttcc ggtgctggta attggcggtg ggacaaacct tctggttggt
     4321 gatctt
//
LOCUS       DAOSRI010000146         4323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18369_length_4323_cov_3.912137, whole genome shotgun sequence.
ACCESSION   DAOSRI010000146 DAOSRI010000000
VERSION     DAOSRI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18369_length_4323_cov_3.912137"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="PK717_05205"
     CDS             complement(<1..2010)
                     /locus_tag="PK717_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR47094.1"
                     /translation="MNKFVTLILCLAMVLQFSHGTARNVEPKKDPIFYEWAASTQKEL
                     SLKNVPKTGFKPFFFPHTTNTAIASYYRGKFDWDGTGTIRIKFGAVNFFCKLWVNSKE
                     AGSHLGGYLPFEFDITGLLKKGKNELVLGAQGILGVVPEIGAKDPRAIKENSVMLPIG
                     SSFMIGGMWLPSSIKKLPSSFIEKVLIDTSVRQKTLKARVGLGGAAEGKKGLSLSLWV
                     EDIQGAKLFALGSKNVDTGKEIPLQEFKASWNNPKLWSPENPNLYFLVSVLSQNGKEI
                     HKDKTRFGFREFWVQGGNFFLNGKKIHLRATSRHLFSQRWADIDIEETAKQVIQKAKA
                     FNSNILRLHANPYPEAFLEEADAQGIMIVDESAAWTFGGAYALENDVFWKNLYRQWDD
                     HIKRDYNHPSFVLASAENELMLTGGAKRKNIQENLQKLGKYIRESSGRPVMFEGDYDP
                     NGSADAINLHYPWEPSSHFTYPNDAYFLDGSFFTDIYPAKEIKWDRSKPMYIGECLWM
                     PGPFHEYAVTGGDYTYQDLKKGYYESKKELFADYIQAFRELEVSAFCPWNPFEDENEK
                     TISASQLTELQSVVSNCYKPVRFFIRQKDDWHYAGSKIFRTVTIQNWSEQPKDLKLAW
                     SFGKGVDSSKSGELSFRLGPDETKTITITLELPGGLVFGKQGSWED"
     gene            complement(2076..3584)
                     /locus_tag="PK717_05210"
     CDS             complement(2076..3584)
                     /locus_tag="PK717_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOR47095.1"
                     /translation="MNDEENIIRELASAWEQLSIFYDWNSQIEDTCGEKQFQKILTRL
                     TKSTGSSCGIAAKLGRGRGTIIIASHSPNFENINPFLEHFKNSLPDIEEKLVRGQRAA
                     DKFMLLGTEHHMLVIPWRKEKRLKGVFIFVRPDLPYNRAESILLSNATYEVSLLIDYA
                     NLSSRFQEKNNELGILVIKNLENYKEIFLEAEKIGSPDTAICNMLLPEDTKLLGISMN
                     RVFFSSKQEDGGKSNDIPSKEILAKLEPPVDKDFSSADGRALSVRHHKDSRGTAFATV
                     VETATAPSPQEIEKLHKIAELATKEALRKDFLGACMQEAYASALFSMSTMIKSSNPQA
                     YSTLENTERLISILSGALCLDENEKKILEKSLMLVDISLIYMDHGALEEYLVKGTTVS
                     GPQTMKTIMEHPIKSAELVQPVKSLSECVPVIRTHHERWDGYGYPMGLKGEEIPKLSR
                     ILVFVQALSSRIAQHSMDLKDFVLVESERVWLSRQAGRAFDPEIVKTFLESF"
     gene            complement(3581..3964)
                     /locus_tag="PK717_05215"
     CDS             complement(3581..3964)
                     /locus_tag="PK717_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR47096.1"
                     /translation="MEPKKSIAVVEDEIAISKLITYVLESAGFQVRAIFDGGEALEKI
                     KENPPDLVLLDLMLPTISGFELLSRLKGEPCLGKLPVIVLTCRGQDEDKDRAFRLGAL
                     EYVTKPFSPTGLVTILKNILSSSVQ"
BASE COUNT          987 a          996 c          996 g         1344 t
ORIGIN      
        1 gtcctcccat gagccctgtt tcccaaaaac cagcccgcct ggcagttcga gtgttattgt
       61 gattgtcttt gtttcatcag ggccaagcct gaatgagagt tcgcctgatt ttgaggagtc
      121 aacccctttc ccgaagctcc aggccagctt gaggtctttt ggctgttctg accagttttg
      181 gatggtcacg gtcctgaaga tttttgatcc tgcgtagtgc cagtcgtcct tttgccttat
      241 gaaaaacctc actggcttgt agcagttgct gacaacactt tgcagttcgg tcagctggct
      301 tgcagaaatc gttttttcat tctcgtcctc aaaagggttc caggggcaaa acgctgacac
      361 ctcaagctcc ctgaatgcct ggatgtagtc ggcgaagagc tccttttttg attcgtaata
      421 gccttttttc aggtcctggt atgtgtagtc accgcccgtc accgcatact catggaacgg
      481 gccgggcatc cacagacatt ctccgatata catcggtttt gatctgtccc atttgatttc
      541 tttggctgga tagatgtcag tgaagaatga cccatccagg aaataggcgt cattcggata
      601 ggtaaaatgt gaggaaggtt cccaggggta atgcaagtta attgcgtctg cactgccatt
      661 tgggtcataa tcaccctcga acatgaccgg cctgcctgag gattccctga tgtatttgcc
      721 aagtttttgc aggttttcct gtatattttt cctctttgcg ccgcccgtca gcatcagctc
      781 gttttctgca gaggcaagca caaatgaagg atggttgtag tctcttttga tgtggtcgtc
      841 ccactgcctg tagaggtttt tccagaacac gtcgttttca agcgcataag caccgccaaa
      901 ggtccatgcg gctgattcgt caactatcat gattccctgg gcatcagcct cttcaaggaa
      961 ggcttccgga taaggattgg catgcaatct taggatgttg gaattgaatg ccttggcttt
     1021 ctggatgacc tgctttgcag tttcttcaat gtcaatatcg gcccatcgct ggctgaatag
     1081 gtgccttgag gtcgccctca agtggatctt cttgccattc agaaaaaaat tcccaccctg
     1141 gacccagaac tccctgaaac caaagcgtgt tttgtctttg tggatctcct tgccattttg
     1201 cgaaaggaca gaaacgagga aatacaggtt tgggttttcc gggctccaaa gcttcggatt
     1261 gttccagctt gctttgaatt cctgaagcgg gatttctttg cctgtgtcaa catttttgct
     1321 gccaagtgca aatagtttcg caccctggat gtcttccacc cacaatgaga gggaaagacc
     1381 ttttttgcct tctgctgcac cgccaagccc gactctggcc ttcagggttt tttgccttac
     1441 cgacgtgtca atcaatactt tttcgataaa gctggatggc agttttttga ttgatgaagg
     1501 gagccacatt cctccgatca tgaaggatga cccaatcggg agcatgaccg agttttcctt
     1561 gattgccctt gggtctttgg cgccaatttc tggcacgaca ccaagaattc cctgtgctcc
     1621 gagcacaagc tcgtttttcc cttttttcag tagaccggtg atgtcaaact caaacggcag
     1681 atacccgcca agatgtgatc cggcctcttt tgagttcacc cacagcttgc aaaagaagtt
     1741 tactgcacca aattttattc taattgttcc tgtgccgtcc cagtcgaatt ttcccctgta
     1801 ataggatgct atggcagtgt ttgtggtgtg tgggaagaaa aatggcttga atcctgtttt
     1861 ggggacattt ttcagtgaca gctctttctg ggttgatgcc gcccactcat aaaaaattgg
     1921 gtcttttttt ggttcgacat tcctggctgt gccatgggaa aactggagca ccattgcaag
     1981 acacaaaata agggtcacaa atttgttcat tgaagcgccc ctttcatttg gttatttagg
     2041 atataatacg ccatctgaaa ggaattggtc gcatcctaaa aagattccag aaacgttttt
     2101 acaatctctg ggtcaaaagc cctccctgcc tggcgtgaga gccacaccct ttcactttct
     2161 acgagaacaa aatctttcag atccattgaa tgctgggcaa tccttgaaga aagggcttgc
     2221 acaaagacaa gtattcttga aagtttcggt atctcctcac cttttagccc catgggatag
     2281 ccatagccgt cccatctttc gtggtgagtg cggatcacag gaacacattc tgagagtgat
     2341 tttaccggct ggacaagctc tgctgatttg attggatgtt ccattatcgt tttcattgtt
     2401 tgaggcccgg agacagttgt ccctttcaca aggtattctt caagtgcccc atggtccatg
     2461 tagataagcg atatatcaac aagcatcaac gatttctcaa ggatcttttt ttcattttca
     2521 tccaggcata atgcccctga aagtatggag atgagtcttt cggtgttctc cagtgtggag
     2581 tacgcctgtg ggtttgatga ttttatcata gtggacatgg agaataaggc cgaagcgtat
     2641 gcttcttgca tgcatgcccc caaaaaatct tttctgagcg cttcttttgt ggcaagctca
     2701 gcaattttat gtaatttttc aatttcctgt ggtgatggtg cagtggcagt ttcgactact
     2761 gttgcaaacg ctgttcccct gctgtcttta tggtgcctga ccgatagcgc cctcccatct
     2821 gcagaagaaa aatctttgtc cacgggaggt tctagtttgg caagaatctc ttttgatgga
     2881 atatcgttgg atttcccgcc atcctcctgc tttgatgaga aaaataccct gttcatggaa
     2941 ataccaagga gttttgtgtc ttctggcagg agcatgttgc aaatggcagt atccggggag
     3001 ccaatttttt ccgcctcaag gaatatctct ttgtagtttt cgagattttt gatgacgaga
     3061 atgcccagtt cattgttctt ttcctggaac ctcgatgaga ggtttgcgta atcaatcaac
     3121 agtgatactt cataggtggc attcgagagc aagattgatt cagccctgtt gtagggcagg
     3181 tccggcctta cgaaaataaa cacacccttg agcctctttt cttttctcca tgggatgaca
     3241 agcatgtggt gttcagttcc aagaagcata aatttatcgg cggccctttg accacgtacc
     3301 agtttttctt caatatctgg cagtgaattt ttgaaatgtt ccaaaaaggg gttgatgttt
     3361 tcaaaattgg gcgaatgcga tgcaataatt attgttcctc ttcctcttcc gagttttgcc
     3421 gctattccgc atgacgagcc agttgatttt gtcagcctgg taaggatttt ctggaactgt
     3481 ttttcgccgc atgtgtcctc aatctggctg ttccagtcat aaaatattga tagttgttcc
     3541 caggccgagg caagctcccg gatgatgttt tcttcgtcgt tcattgtaca ctgcttgaga
     3601 ggatattttt aagtatcgtc acaagccccg ttggagaaaa gggtttcgta acatattcta
     3661 gcgcaccgag cctgaaagcc ctgtctttgt cctcatcctg ccccctgcag gtgagcacta
     3721 taacagggag cttgccaagg caaggttccc ccttcagtct ggagagcaac tcgaaaccgg
     3781 atatcgttgg tagcatgagg tccaacaata ctagatctgg agggttttct tttattttct
     3841 caagcgcctc cccaccatca aagattgccc ttacttggaa accggcgctt tcgagcacat
     3901 aggtaataag ttttgagatt gcgatttcat cttcgacaac agcaatgctc ttttttggct
     3961 ccatctgtct agatgataat tttttgctgt ctgtaagtca aatcgtttga tgtatgcaaa
     4021 acgctttatt tgttgtttga aatggcagtt gagtttttaa gataattttt gttgggtgct
     4081 tgattggtaa tgatctgttg ataatgagtt tacaaaaaat ccccacagcc attctttgtt
     4141 tactgaaaca gctgtgggga tttagcagct actttctgtt ccagggcctc aatggatgtt
     4201 tggccagtac ataggaggca agcggaatgg tttttccggt tttctcgtca aagagtggcg
     4261 ttgcgcccat gtacatcatg tccccagggt caccatactg gtttaatcct tgctcttgtg
     4321 ccc
//
LOCUS       DAOSRI010000147         4294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18525_length_4294_cov_3.514980, whole genome shotgun sequence.
ACCESSION   DAOSRI010000147 DAOSRI010000000
VERSION     DAOSRI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18525_length_4294_cov_3.514980"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK717_05220"
     CDS             complement(<1..442)
                     /locus_tag="PK717_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HOR47097.1"
                     /translation="MDKAVFGRIIRLDEELRKAGIEKKTVDKIMEGGDKILKTSKPAD
                     KAAWLKIAMDRMDSLLPEETRTKVREECACCTTGSRLKLMKKLSEENKDLDSFFKAVD
                     NSHVFGKKVEKKGNKVYVNFGGSKCVCTPKVSNEIFSTTYCQCCK"
     gene            complement(493..1632)
                     /locus_tag="PK717_05225"
     CDS             complement(493..1632)
                     /locus_tag="PK717_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47098.1"
                     /translation="MRNRENKLIIALICVVIALSSVSARIARGEPSFAKLWEIRRTIP
                     LVAKDGVIVAGWPNMENQTLFACFDSKTGNQLWKEGKREPINWDAIRADNFQLEGDRL
                     FVLQREGDKYSFNCFNKNDGKQIWSLGMLDDINPEKMPFVDGNNFYLIQHGIIQCFDS
                     ENGQLIWKAGTGSGPLKRLAGTYKDLQFWTGSHIMCLDLKTKSQLWATPQNDCPLAPK
                     NLDGMTGNTPCFVGDYTFFLGHDCDVAGGLSIFCRNIFTGKTKAKLDRPFGMAATKEF
                     FISIVSTGQGNFSMYAFDPESGTKVWETRLERTAREFMFSLGDKFVRTKFGENQTTLA
                     ILCDAKTGRELSTTNLGEGVTNIIQDGELLFVESKNKTTCYKISG"
     gene            complement(1669..3045)
                     /gene="mgtE"
                     /locus_tag="PK717_05230"
     CDS             complement(1669..3045)
                     /gene="mgtE"
                     /locus_tag="PK717_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR47099.1"
                     /translation="MKNEIREQLLSLLEEGKLKAIEEYCAEFSSSDIAEAIAELDPET
                     MVRILRGIEIHQSGEVMGYLDLDTQVELAEKLKKEDLASLISHMLSDEGADLINELPE
                     PLSNSLMPLLAKAKREQLKSLAEQEEGTCGALMSADYVTVGPNMTIAQATEKVRLDGP
                     HKETIYYIYVVDGTNRLLGMISLRNLIVNDPRKKVADVMFEDLEMVKTTDPQEKAAEI
                     IQKYDLIAIPVINDRDQLVGVITHDDAADVITEEQTEDILGMGKIVPTAGEMAYLKSS
                     PFQNFLKRIPWVASLAALGLISGTILHTFQDSLSKLIILALYMPMLADTGGNCGTQSS
                     TVIIRALALKEITPKHFFRIIGKEFATGIMMSVLLGLLAFLKVILLSSGEVLPAGFTL
                     INVGIIISLALSIQVVTSTVVGATLPLVVSAIKLDPALIASPALTTIVDMSGLLIYFS
                     LAKLLLGL"
     gene            complement(3329..4147)
                     /locus_tag="PK717_05235"
     CDS             complement(3329..4147)
                     /locus_tag="PK717_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOR47100.1"
                     /translation="MNSDLLHERLEQLYQQYAKKEFVHPDPLEFLYLYQDTGDREIVG
                     LIASSLAYGRVAQILKSVRKVLDVLGNSPRLFLLETSPSLLEKFFAGFKHRFTTGEEI
                     VAMLLGTRHALKEFGSLNECFVHGLKKSRDEMVEAQCFFTSSITGGGCKNSLVPAPEK
                     DSACKRLNLYFRWMVRDSDVDPGGWNGVPKKGLIVPLDTHMFNICRKLGLTDRNQANL
                     KTALQITENFRKFDPDDPTRYDFPLTRLGIRTDTDLDGFLTSCGVECTTKKQTK"
     gene            complement(4144..>4294)
                     /locus_tag="PK717_05240"
     CDS             complement(4144..>4294)
                     /locus_tag="PK717_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOR47101.1"
                     /translation="YTCNVCGYVYDPAKGDPDNGIPAGTPFEKLPDNWVCPICGAAKS
                     EFSPE"
BASE COUNT          990 a         1022 c          981 g         1301 t
ORIGIN      
        1 ccttgcagca ctggcagtaa gtcgtggaga atatctcgtt tgacaccttt ggcgtgcaaa
       61 cgcatttgct gccgccaaaa ttcacataga ccttgttgcc ctttttttca accttcttgc
      121 caaaaacgtg cgaattgtcg actgctttga aaaaactgtc caggtctttg ttttcctccg
      181 aaagtttttt catgagcttg agcctgcttc cagttgtgca acatgcacat tcttccctga
      241 cctttgttct ggtttcttct ggcaggagcg aatccatcct gtccattgcg attttgagcc
      301 aagccgcttt gtcggccggt ttggaggttt ttagtatttt gtccccaccc tccattatct
      361 tgtcgaccgt cttcttttcg atgcccgctt ttctcagctc ctcgtcaagc ctgattattc
      421 ttccaaatac agctttgtcc atttcctgtc tctcctttgt atttttttga gaataacaca
      481 tgcgctgttt tgtcacccag aaattttgta gcaggtcgtt ttgtttttgg attcaacaaa
      541 gaggagctca ccatcctgaa tgatgtttgt aacaccctct ccgaggttgg ttgttgaaag
      601 ctccctgcct gtttttgcat cacacagaat agcaagtgtt gtctggtttt ctccaaattt
      661 tgtccggaca aatttgtccc caagggagaa cataaattcc ctggccgttc tctctaacct
      721 tgtttcccaa accttggtcc ccgactctgg atcaaacgca tacatgctga aattgccctg
      781 cccagttgat acaattgata tgaaaaattc ttttgtggcc gccattccaa atggcctgtc
      841 aagttttgcc tttgtttttc cggtgaagat gtttctgcag aatatggaca atcctcctgc
      901 aacatcgcaa tcatggccaa ggaaaaatgt atagtctcca acaaagcagg gggtatttcc
      961 cgtcatgcca tcaagatttt ttggggcaag cgggcagtcg ttctggggtg ttgcccacaa
     1021 ttgtgacttt gttttcaggt ctagacacat aatatggctc cctgtccaga actggagatc
     1081 tttgtatgtc ccggccagtc gcttcaatgg cccggatcct gtgcctgcct tccagatcag
     1141 ttgtccattt tctgaatcaa aacactggat gatgccgtgc tggatcaagt aaaaattatt
     1201 cccatccacg aatggcattt tttccgggtt gatatcatca agcattccaa gtgaccatat
     1261 ttgcttgcca tcatttttgt tgaaacaatt gaatgagtac ttgtcacctt cgcgctggag
     1321 aacaaaaagc ctgtcacctt caagctggaa attgtcggcc cttatggcgt cccagttaat
     1381 gggttccctc ttgccttctt tccacaactg gttgccagtc ttggaatcaa agcatgcaaa
     1441 aagggtctgg ttttccatgt ttggccagcc tgcgacgatg acaccgtctt ttgctacgag
     1501 ggggattgtc cttcttatct cccagagctt tgcaaaggat ggctcgcctc ttgcgatccg
     1561 ggcactcaca gatgacaggg ctatgactac gcaaattagt gcaattatca gcttgttttc
     1621 tctgtttctc accttgggtg gaattttatt ccatagtcca tccaaatgct atagcccaag
     1681 cagtagcttg gccagcgaga agtagatcaa aagtccgctc atgtcaacga ttgttgtcaa
     1741 tgcagggctg gcaatgagtg caggatcaag cttgatcgca gacacaacaa gcggcaatgt
     1801 ggcaccgacg acagttgatg ttacaacctg gattgagagg gcaagagaaa taattatgcc
     1861 gacatttatc agtgtaaacc ctgctggtag tacctccccg ctggaaagca gaatcacttt
     1921 gaggaaggcg agcagtccca aaagaacact catcatgatt cctgtggcaa attccttgcc
     1981 tattatcctg aagaaatgct ttggcgttat ctctttgagt gcgagtgccc ttatgatgac
     2041 cgttgaagat tgggtgccgc agttaccacc ggtatcggcg agcattggca tgtacagggc
     2101 aagtatgatc agtttggaaa gggagtcctg gaaggtgtgg aggatggttc cacttatcaa
     2161 gcccaatgcg gccaacgagg ccacccacgg tatccttttg aggaagttct ggaacggaga
     2221 tgatttgagg tatgccattt cccctgccgt tggaacgatt tttcccattc caaggatgtc
     2281 ttctgtttgc tcctcggtta tgacatctgc agcgtcgtca tgcgttatca cgccaacgag
     2341 ctgatctctg tcgtttatta cgggaatggc aataaggtca tatttttgta ttatctctgc
     2401 agctttttcc tgaggatcgg ttgtttttac catctcaagg tcctcaaaca tgacatcggc
     2461 cacttttttg cgcggatcat ttacaatgag gtttctgaga gagatcatgc ccaagagcct
     2521 gtttgtgcca tcaaccacat aaatataata gatcgtttct ttgtgtggtc catcgagtct
     2581 gactttttct gtcgcctggg ctattgtcat gttgggccca accgtaacgt aatcagcact
     2641 cataagggcg ccgcaagttc cctcttcctg ttcggctagc gatttgagct gttctctttt
     2701 tgcttttgca agcaatggca ttaggctgtt cgagaggggt tcgggcaatt cattgatgag
     2761 gtctgcgccc tcatcggaaa gcatgtgaga aatgagcgaa gcgaggtcct cttttttcag
     2821 tttttcggcc aactctactt gtgtgtcaag atcaagatag cccatgactt ccccggactg
     2881 gtgtatctca atgccacgga gaattctgac cattgtttct ggatcgagtt ctgcaatagc
     2941 ctctgcaatg tcagatgagg aaaactctgc gcaatattct tcaattgcct tgagtttgcc
     3001 ctcttcaagt agtgacagga gttgttccct gatttcgttt ttcatgcgct tgcctctaaa
     3061 agtgatttga tggcatgagg caagcttaag aaggggttag gccggcctca tggcgctgtt
     3121 tgtacaacta gggtaagggc tagggctatc agattctata gcttccatga ggacctcctt
     3181 ctttttttgc tttttatgta gttaaacagt atgcccatgg tttgtcaaca ctattttatt
     3241 tagctcctga aattcgctcc aatacaacat tagtggacat cttggaattt gacctggttg
     3301 gaggggttaa aagattattt tgaaggattc attttgtttg ttttttggta gtacattcaa
     3361 ctccgcatga agtaaggaat ccgtccagat cagtgtctgt tcttattcca agccttgtga
     3421 gcggaaaatc ataccttgtg ggatcatcag ggtcgaattt cctaaaattt tccgttatct
     3481 gaagggccgt tttcagattt gcctgatttc tgtccgtgag gccaagcttt ctgcaaatgt
     3541 tgaacatatg cgtgtccaga gggacgatta gccccttttt tggaacacca ttccagcccc
     3601 caggatcgac atcgctgtcc cttaccatcc acctgaaata caggttcagc cttttgcagg
     3661 cggaatcttt ttcaggtgct ggaacaaggc tgtttttgca ccctccgcct gttatgctgg
     3721 acgtgaaaaa gcattgcgct tcaaccatct catcgcgcga ttttttcagg ccgtggacaa
     3781 aacactcgtt aaggctgcca aactccttca aggcatgtct tgttcccaaa agcatggcga
     3841 caatctcctc accagtcgta aatctgtgct tgaatcctgc aaagaacttt tcaaggagac
     3901 tgggagaagt ttcaagaaga aacagccttg gtgaattgcc aagaacatcc aggaccttcc
     3961 ggacagactt gaggatctgt gcgaccctgc cataagccag cgatgaggca atgagcccaa
     4021 ctatttccct gtcgccagtg tcctggtaaa gatacaggaa ttccagcggg tcaggatgca
     4081 caaactcttt tttagcgtat tgttgataaa gctgctccaa cctttcatgg agcagatcac
     4141 tgttcactcc ggcgagaatt ctgacttggc agcgccacag attgggcaga cccaattgtc
     4201 agggagcttt tcaaatggcg ttcctgccgg gattccgttg tccgggtcgc cttttgccgg
     4261 gtcataaacg tagccgcaaa cgttgcatgt gtac
//
LOCUS       DAOSRI010000148         4292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18537_length_4292_cov_4.118716, whole genome shotgun sequence.
ACCESSION   DAOSRI010000148 DAOSRI010000000
VERSION     DAOSRI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18537_length_4292_cov_4.118716"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK717_05245"
     CDS             complement(<1..186)
                     /locus_tag="PK717_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR47102.1"
                     /translation="MGTITRISGPLVEARDLNEAFMYELVKVGEMGLFGEIIEMRSGI
                     VSIQVYEDTETLKPGEPV"
     gene            complement(170..508)
                     /locus_tag="PK717_05250"
     CDS             complement(170..508)
                     /locus_tag="PK717_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOR47103.1"
                     /translation="MSLNVVAIGNALQVSPWGFLGAKVIIPEKEDEQKELSGLLSDEK
                     IGILLISNDLAVRNQHLLEKQHLHGPVVLVIPDGFEEDDPAGEALRKLVMEAIGVDLL
                     GKERLNGNNN"
     gene            complement(498..1406)
                     /locus_tag="PK717_05255"
     CDS             complement(498..1406)
                     /locus_tag="PK717_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47104.1"
                     /translation="MTKQTGLLSSYPPPDAEAFRFTSARVKVIEGKLLSQADFLRFKN
                     LSPGDRARAIKGIYPWFDGDASSFRAQADRHLFGESISLSEESGHPDLATYFLLPRAF
                     AQVQASLEGATNPPAIVPFLGNYFDSCSISDLPEGLREVVSDAKNLYSTGEKPKARRL
                     MTQKLVDMLMESRFSKLSYFDECINAYSNLLEVRLVIASRIRKGSFVPIEGCEINGDC
                     TPEQLAKTLGTTNPDLENLEQVASKKFFEKVTASRAIPAGPEVIFGYLSELENELVNA
                     SMLLAGSLVALPPAELEMAYGRAYVS"
     gene            complement(1363..1914)
                     /locus_tag="PK717_05260"
     CDS             complement(1363..1914)
                     /locus_tag="PK717_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47105.1"
                     /translation="MGLESLTSIILDGAKSASEKLLEDAKKEADLIISSAQARSREIF
                     AATYAPFEKEMAAKKSSALSMAKMEETSARLDALNKMLYETCKKAMETLFSNPSFLEK
                     VKSWALGHKGGEFFIGKNIDSAMPELKKTLLDKGFNVVTLQNPNAIEHRQEKQIDRLD
                     IEDGLRRLAFDQADRIIVILSAP"
     gene            complement(1914..2411)
                     /locus_tag="PK717_05265"
     CDS             complement(1914..2411)
                     /locus_tag="PK717_05265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOR47106.1"
                     /translation="MNNVLAELFGQSGQGFVILGMIVNVVLGGIGSAKGLRIAATQTA
                     GVMSEKPELFGKLFVLMVLPGTQGFYSFIASFLAGLQTFLGNKTISTPASIGLMLLLI
                     AMGFIELISAIEQGKTSAAAINMVAKQERTFGQAIIMPALVETYAVLGLLISIIVILS
                     INLIK"
     gene            complement(2408..>4292)
                     /locus_tag="PK717_05270"
     CDS             complement(2408..>4292)
                     /locus_tag="PK717_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOR47107.1"
                     /translation="FGTLNTRPFEGLEMANQKAGARKERLEKASESLLFLDVVAPRKM
                     PFTANFAWPRPVLDPKNVKKLLSGNPEAVISELLGQKTRLEQIKTELAKLKERANVLS
                     KFTSLSIPTHIESSEVDYFIGVVQPGREKQATALNGEKEFAIWASKGVIVMASLKSRS
                     AEARELLAKFGFAALPFPPSKKTVREQLQDTNSQIASLEKEKQSLEKEASKNAKDKRL
                     AIMLWMDNEETAQRLEEVPALSGIFGSFVVGYVPAKDAKKFEQTVSQKCPWTRLVLLD
                     VPEDEEPPVKLKNNPYSTPFETVTSMYGMPSYRGIDSTFMVSILFPIFFGFCFGDALY
                     GIMLVLFSLWYLRTFKTEEGGKRFFRLFIICGVSTFIIGALTGSWGGNLIASDGVIAV
                     PALVALKDRITLLDPLKQTITFFVLTLFIGIGSQFIGILIAAWMNIRQGKWLDALLDQ
                     ASWIIFLSGLVMFGIDFLGGNLTGTPKTVMFVLILTGAFFLVFFQGRGSKNFFGRIGL
                     GLISLYGIMGGYGTASFIGDIMSYSRLMALGLTSSIVALAFNKMAMMIGFSNPFGSIF
                     TIVILVFGHLLNFFLNIIGSFVHPARLTFLEFYSRFYSGGGRSFSPLEKSEKRIKIME
                     EKA"
BASE COUNT         1013 a         1055 c         1053 g         1171 t
ORIGIN      
        1 gaccggttcg cccggtttga gcgtttcggt gtcctcgtac acctggattg aaacaatgcc
       61 cgagcgcatt tctattattt cgccaaaaag gcccatctcg ccgaccttga cgagttcata
      121 catgaaggcc tcgttcaggt ccctggcctc gacaagcggg cctgaaatcc tagttattgt
      181 tcccattgag cctctctttt ccgagcaggt caacaccaat tgcctccatg acgagttttc
      241 gcagtgcctc gccagccgga tcgtcttcct cgaagccgtc cggaattacc aggacgaccg
      301 gcccgtggag gtgctgtttt tcaagaaggt gctggtttct gaccgccaga tcgtttgata
      361 tcagcaatat tcctattttt tcatcagaaa gaagaccgga aagttctttt tgctcgtctt
      421 ccttttccgg gatgattact tttgcaccca aaaaacccca gggtgatacc tggagggcgt
      481 ttcctattgc cacaacatta agagacatag gccctcccgt atgccatctc gagctctgct
      541 ggcgggagcg cgaccagcga acctgcaagc agcatcgagg cattcaccag ctcgttctcg
      601 agctctgaca ggtatccgaa gatgacctcg gggcctgcag gaatggccct tgaggccgtt
      661 actttttcaa aaaacttttt gcttgcaacc tgttcgaggt tctcaaggtc agggtttgtt
      721 gtgccaagtg tttttgcgag ctgttccgga gtgcagtcgc catttatttc acagccttca
      781 attggcacaa aagagccctt ccttatcctc gatgcgatca caagcctgac ttcaagaagg
      841 tttgaatatg cattgatgca ctcgtcaaaa tatgaaagtt tggaaaatct tgattccatt
      901 agcatgtcta ccaatttttg ggtcatcagg cgccttgctt ttggcttctc acctgtactg
      961 taaaggtttt tcgcatcaga aaccacttcc ctcaggcctt caggaaggtc agaaatggag
     1021 cacgaatcaa aataattgcc aagaaaaggc acaattgcag gcgggtttgt cgcaccctca
     1081 aggctggcct gcacctgggc aaatgccctt ggaagaagga agtatgttgc aaggtccgga
     1141 tggccggact cttccgaaag ggagatgctc tccccaaaaa ggtgcctgtc tgcctgtgcc
     1201 ctgaagctgc tagcatcgcc atcgaaccat gggtaaatgc cttttatggc cctcgccctg
     1261 tcaccagggg aaaggttttt gaacctcagg aagtccgcct gcgagagcag tttgccctcg
     1321 atgactttca cccttgccga agtgaagcgg aaggcctcgg cgtcaggggg cggataggat
     1381 gacaataatc ctgtctgctt ggtcaaatgc cagcctcctt aagccgtctt caatgtccag
     1441 cctgtctatc tgtttttcct gcctgtgctc gattgcattg gggttttgaa gagtaaccac
     1501 attaaaacct ttatccagca gtgttttttt gagttcgggc attgcgctgt caatgttctt
     1561 gccgataaag aattcgccac ccttgtgccc aagcgcccag gattttacct tctccaggaa
     1621 cgacgggttt gagaacagtg tttccatagc ctttttgcag gtttcgtaca gcatcttgtt
     1681 gagagcgtcg agccttgccg aggtctcttc catcttggcc atggagagtg cggacgattt
     1741 tttggccgcc atttcttttt caaaaggagc ataggtggcc gcaaaaatct ccctgcttct
     1801 ggcctgggca gagctgatta tcagatctgc ttcttttttg gcgtcctcga ggagtttttc
     1861 cgaggctgat tttgccccat cgaggattat tgaagtcagt gattcaaggc ccattatttt
     1921 atcaaattga tcgagaggat gacgatgatc gagatgagca ggccaaggac tgcgtatgtt
     1981 tcaacaaggg ccggcatgat gattgcctgg ccgaatgtcc tctcctgctt ggcgaccatg
     2041 ttgattgccg cggcggaggt cttgccctgt tcaatggctg aaatcaattc gataaagccc
     2101 atggctatga gaaggagcat caatcctatt gaggccggtg tggagattgt tttgttgcca
     2161 aggaatgttt gaaggccggc caaaaatgag gctatgaaag aatagaagcc ctgtgttccc
     2221 ggaaggacca tcaaaacaaa cagttttcca aagagttcag gtttttcgga cattacacct
     2281 gccgtttgtg ttgcggctat cctgagaccc tttgcagatc ctatgcctcc aagcaccacg
     2341 ttgactatca ttcctaggat aacgaagccc tgtcctgatt gcccgaaaag ttctgccaat
     2401 acattattca tgctttttcc tccataatct ttatccgttt ttcgcttttt tccaaaggag
     2461 agaaactgcg accgcctcca gaatagaatc tggaataaaa ctcaaggaac gttagtcttg
     2521 cagggtgcac aaaactccca atgatgttca ggaaaaaatt gagcaggtgg ccaaaaacaa
     2581 gtattacaat cgtgaaaatt gaaccaaagg gatttgaaaa tcctatcatc atggccatct
     2641 tgttgaatgc cagggccaca atggagctgg taagaccaag cgccatgagt cttgagtagc
     2701 tcattatgtc accaataaat gatgcggtgc catacccgcc catgattccg tagagggaaa
     2761 tgagacccag acctatcctg ccgaagaagt tttttgaacc cctgccttga aagaacacca
     2821 ggaagaaagc gccggtaagt atcagcacaa acatcaccgt ttttggggtg cctgtcagat
     2881 tgccgcccag aaagtcgatt ccaaacatga ccagaccgga gaggaagatt atccagcttg
     2941 cctggtcaag cagtgcgtcc aaccatttgc cctgccttat gttcatccat gcggcgatga
     3001 ggatgccgat aaactggctc cctatgccga taaagagtgt caggacaaag aatgttatgg
     3061 tttgcttcag gggatcgagc agggttatcc tgtctttcag tgccactagc gcaggaactg
     3121 ctatgacgcc atcggaggcg atcaggttgc caccccagga gccggtgagt gcgccaatga
     3181 tgaaggtgga taccccacag atgatgaaca gcctgaaaaa cctttttccg ccttcttctg
     3241 ttttgaatgt tctcagatac cacaaggaaa acaggacaag cattatgccg tatagtgcat
     3301 cgccaaaaca gaacccaaaa aatatcggga acaaaatgga gaccataaaa gttgaatcaa
     3361 tgcctctgta ggatggcatc ccatacatcg aggttaccgt ttcaaaaggc gtgctgtatg
     3421 ggttgttttt gagctttaca ggcggttcct cgtcttccgg aacatcaagg agcaccagtc
     3481 tggtccatgg gcacttttgg gaaaccgttt gctcaaattt tttcgcatct tttgccggaa
     3541 cgtatccaac gacaaaactg ccaaaaatac cggacagggc tggtacttct tcgagtcttt
     3601 gggccgtttc ctcgttgtcc atccagagca ttatggcaag ccgtttgtct ttggcattct
     3661 tgctagcctc tttttcaagc gactgttttt ctttctccag gctggcaatt tgggagtttg
     3721 tatcctgcag ttgttccctg acagttttct tggagggtgg gaaaggaagg gccgcaaatc
     3781 cgaattttgc cagcagctcc cttgcctcgg ctgatctcga cttgagtgag gccatgacaa
     3841 tgacgccctt tgatgcccag atggcaaatt ccttttcacc attcagtgct gtggcctgtt
     3901 tttccctgcc cggctgcact accccgatga agtaatcaac ttcactgctt tcaatgtgtg
     3961 ttggaatgga gagcgaggtg aattttgaaa gaacatttgc cctttccttg agtttggcca
     4021 gttctgtttt tatctgttca agtcttgttt tttgcccaag cagttccgat atgacggctt
     4081 ctgggttgcc tgagagcagt ttttttacgt tttttgggtc aaggaccggc cttggccagg
     4141 caaagttggc tgtgaagggc attttgcgtg gtgcgaccac atcaaggaaa agcagtgatt
     4201 cagaggcctt ttcgaggcgc tcttttctag cgccggcttt ttggttggcc atctccagac
     4261 cttcaaaagg tcttgtgttc agggtgccaa at
//
LOCUS       DAOSRI010000149         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18547_length_4291_cov_3.449717, whole genome shotgun sequence.
ACCESSION   DAOSRI010000149 DAOSRI010000000
VERSION     DAOSRI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18547_length_4291_cov_3.449717"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /gene="guaA"
                     /locus_tag="PK717_05275"
     CDS             complement(<1..1498)
                     /gene="guaA"
                     /locus_tag="PK717_05275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOR47108.1"
                     /translation="MIAIVDFGSQYTQLIARRVREAHVYCEVVPYWTKPDELSKYKGI
                     ILSGSPRSVLGESSPMPDSSIYYLGKPVLGLCYGMQAMAHQLGGAVEKGKSGEYGRAE
                     AKLDRNSVLFQGTKEIGTVWMSHGDHVIKAPKDFEVIASSGSCPVAAISNVSKNFYGL
                     QFHPEVKHTEDGALIIENFLFKICGEEPSWKMSSFAEEQIEAIKKTVGGDHVIAGVSG
                     GVDSVVTAVLLHKAIGKQLHVIFVDHGLLREGEREEVIFTLTNLGLDPILVGCEDIFL
                     DALKGVTEPEQKRTIIGRLFIEEFEKACKTIPGVKWLAQGTLYPDVIESSGGVSGLAD
                     KIKSHHNVGGLPERMNLKLIEPMRMLFKDEVRHLGRELGVPKHLTDRQPFPGPGLAIR
                     LVGEVTRQRLDILRKADEIVREEIAGDADQYFAVLLPVSSVGVQGDERSYNCVCAVRA
                     VSTTDFMTASWTKLPYEVLDRIATRITGEVKQIGRVVFDITNKPPATIE"
     gene            complement(1495..2013)
                     /gene="def"
                     /locus_tag="PK717_05280"
     CDS             complement(1495..2013)
                     /gene="def"
                     /locus_tag="PK717_05280"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOR47109.1"
                     /translation="MRKVLDCDSPILRKKSKPITKIDDNLIELIKEMFVIMKTTAPKG
                     IGFAAPQIGELIRLVVIDYGEGEMVFINPEILSGEGECIYKEGCLSIPGLWADIKRAE
                     KVRFACTNLRGKRVEGEATGILARVLQHEIDHLDGKLFTDYIEPGQEIELDEGVTLPK
                     RFQEKLMKGDKK"
     gene            complement(2010..4196)
                     /gene="priA"
                     /locus_tag="PK717_05285"
     CDS             complement(2010..4196)
                     /gene="priA"
                     /locus_tag="PK717_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOR47110.1"
                     /translation="MNVHVMVEGLELTYSVPESLSGRVVPGCLVLVPLRNRTAKGVVV
                     GIGDEEAGIELKEMVSLVYDTPVIDEEGIEATIVADSLAFSKAGTTLSDNLWMPPSPR
                     IETKVEILGECEFSPSEKTLFEKIVQLGDDATLGRLNHALGKSRVTYALRGLVKKGAI
                     RLVEVVSAPLLPKPKLQYEADPDVDSSVNEKAPFCSSMSSLSKASGVSLSKIKKLVLS
                     GKLRLVESAKPGSVPKQEKPGFDVEFFEGLVLEKRVALYADWARGHMASGQSMAIIAP
                     DASLCRAIHKEISKIVDDGVYLATGDGSMANDSRLWDALRSSLPCVVIGMGQALFLPF
                     ANLSRVVVDEPASPHYEQDQPGKLRLGALARVRAKVGKCNLSLVGAPSTLEALKEGAT
                     PVSLPNRVEVVNMLFQAGAKNQPLVSEGMVSAIKKELEAGRPSVVLIARKGYSNFVYC
                     DECNEVISCPTCKVPLMYSLATGKVSCRFCGYEAKAPSTCPSCGGVSVLFKAGGTERL
                     HLELEERLPQCRILRADANTKNAFANIRSFGLPGDVLVGTTMVLDRADFSNVRLACIA
                     SLDGVMSMPVFSASHKAYSLVAILAGKLSSGRVLLQTYMPHHPLIKSLVSSDASTFLM
                     QELEDRKESFYPPFSQVLWWIVTSKEQGEAERWAKSCYETLQELVKDATILPPNATYY
                     HRLKGEYRWDILLKIKDLEGAVPSLWEAMKLCRMKGIKLEVVNPNQ"
BASE COUNT          965 a         1197 c         1026 g         1103 t
ORIGIN      
        1 actcgatcgt ggctggtggc ttgtttgtga tgtcaaaaac caccctgccg atctgcttca
       61 cctctcccgt gatgcgggtt gcaatcctgt ccagcacttc gtagggcagt tttgtccatg
      121 aggccgtcat aaaatctgtt gtggagacgg ccctgacggc gcaaacgcaa ttgtatgacc
      181 gctcgtcacc ctggactcca acggaggaga cgggcaagag gactgcaaaa tactggtctg
      241 cgtcgcctgc gatctcttcc ctcacgatct cgtcggcttt tctcagaata tcaagacgct
      301 gtcttgtcac ctcgccaacg agcctgattg cgaggcccgg gcccgggaac ggctgtctgt
      361 cggtcaggtg cttggggacg ccaagctccc tgccaaggtg gcgcacctcg tccttgaaga
      421 gcattctcat tggctcaatg agcttcaggt tcatcctctc ggggaggccg ccaacattgt
      481 ggtggctctt tattttgtcg gccaggccag agacgcctcc agacgactcg atcacgtctg
      541 gatagagcgt cccctgcgcc agccatttga cgcctgggat tgtcttgcag gccttttcaa
      601 attcctcgat gaagagcctg ccaatgattg ttcttttttg ttccggctcg gtgacaccct
      661 tgagagcgtc caggaaaatg tcctcacagc caacaagaat cgggtccaga ccaaggtttg
      721 tgagtgtgaa tatgacctct tccctctcgc cttcccgaag caggccgtgg tcaacaaaaa
      781 tcacgtggag ctgtttgcca atggccttgt ggagcaggac ggccgttaca acagaatcga
      841 cgccaccaga gacgccggct atgacgtggt cacccccgac ggtttttttg atcgcttcta
      901 tctgctcctc ggcaaaagag ctcatcttcc acgatggctc ctcgccgcat atcttgaaaa
      961 ggaaattctc gattatcagg gcgccgtcct cggtgtgctt tacctcgggg tggaactgca
     1021 gaccatagaa gtttttggag acatttgata ttgccgccac gggacagctt cctgatgagg
     1081 cgattacctc aaaatccttt ggcgccttga tgacatggtc gccatgggac atccagacgg
     1141 tgccgatttc ttttgtgccc tgaaaaagca cagaattgcg gtcaagcttc gcctcggccc
     1201 ttccatactc gccggatttg cccttctcca ctgcaccacc aagctggtgg gccattgcct
     1261 gcattccata gcacaggcca agtaccggct tgccaaggta atatatcgag ctgtccggca
     1321 ttggagagct ctccccaagg actgaccttg gggagccgga cagaataatg cctttgtatt
     1381 ttgatagttc gtcaggcttt gtccagtatg gcaccacctc gcaatagaca tgggcttctc
     1441 tgacccttct tgcgataagc tgggtatact gcgaaccaaa gtctacgatt gctatcattt
     1501 tttgtcgcct ttcatgagtt tttcctgaaa acgctttgga agcgtcacgc cctcatccag
     1561 ttcgatctcc tggcccggct cgatgtagtc agtgaagagt tttccgtcta gatggtcaat
     1621 ctcgtgctgg agtacccttg caagaatgcc ggttgcttcg ccttcgacgc gctttcccct
     1681 cagatttgtg catgcaaatc tgaccttttc ggccctcttg atgtctgccc acaggccagg
     1741 aattgaaaga cagccctctt tgtagatgca ctcgccttcc cccgagagta tctcagggtt
     1801 gatgaagacc atctcgccct caccataatc gatcacaaca agccttatca gctcaccgat
     1861 ctgcggtgcg gcaaaaccga tgccctttgg ggcagtcgtt ttcattatca caaacatttc
     1921 tttgatcagc tcgatcagat tgtcatcaat ttttgttatc ggttttgact ttttcctcag
     1981 tatcgggctg tcacagtcaa gaacttttct cattggttgg gattcaccac ttccagcttt
     2041 attcctttca ttctacacag cttcatcgcc tcccagaggg acggaactgc accttcaagg
     2101 tcctttatct ttaaaagtat atcccatctg tactccccct tcaagcggtg ataataggtt
     2161 gcgtttggag gcaaaatggt ggcatctttc acaagttcct gcagggtttc atagcaagac
     2221 tttgcccatc tttccgcctc gccctgttct ttggaagtga caatccacca aagtacctgg
     2281 gagaatggag gataaaatga ttctttcctg tcctcaagct cctgcataag gaaggtgctg
     2341 gcatcagagg aaacaagcga tttgatgagc gggtggtgcg gcatgtatgt ctggagcaag
     2401 actctgccgg atgaaagctt gccggcaagg atggccacaa gcgaataggc tttgtgggaa
     2461 gccgaaaaaa cgggcatcga catcacaccg tcaaggcttg ctatgcaggc aagcctgaca
     2521 tttgaaaaat cggccctgtc cagaaccatg gttgtgccga caagaacatc ccctggcaga
     2581 ccaaaactcc ttatgtttgc aaaagcattt tttgtgtttg catcggcccg cagtatcctg
     2641 cactgtggca atctctcctc gagctctaag tggagtctct cggtcccacc tgccttaaac
     2701 aggacagaca caccaccaca gctcgggcag gtacttggcg ccttggcctc atagccacag
     2761 aacctgcagg agaccttgcc cgttgcgaga ctatacatga gtggcacctt gcatgtcggg
     2821 caagaaatga cctcgttgca ttcatcacaa tagacaaaat ttgaatagcc ttttctggcg
     2881 atcaggacca cagagggtct gccagcttcg agctcctttt taatcgctga aaccatgccc
     2941 tccgagacca gcggctggtt tttggcgcct gcctggaaca gcatattgac gacctcgacg
     3001 cggttgggaa gcgatacagg agtagcacct tctttcaggg cttccagcgt tgatggggcc
     3061 ccaaccaagc tgagattgca tttgccaact tttgccctca cccttgcaag ggcgcccagc
     3121 ctcagtttgc caggttggtc ctgttcgtaa tgtggagatg ccggctcatc aacgacaacc
     3181 cttgaaagat ttgcaaaagg cagaaacagc gcctgcccca tgccaatcac cacacatggc
     3241 agggaagatc tgagtgcgtc ccagagcctg ctgtcgttgg ccatcgagcc atcgcctgtt
     3301 gcaagataga cgccatcatc aacaattttg cttatttcct tgtgtatcgc cctgcaaagt
     3361 gaggcgtccg gggcgattat cgccatgctc tggccagaag ccatgtgccc tctggcccaa
     3421 tcagcgtaaa gagcaaccct tttttcaagc acaaggcctt caaaaaattc cacatcaaac
     3481 cctggttttt cctgtttcgg gacagagcct ggttttgcac tttctacaag cctgagcttc
     3541 ccggagagca caagtttttt tatcttggag agcgacaccc cgcttgcttt cgagagtgaa
     3601 gacatggaag agcagaaagg tgctttttca ttgactgatg agtcaacgtc cgggtcggct
     3661 tcgtactgga gcttcggttt tggcaataat ggtgcagaaa ccacctccac aagccgtatt
     3721 gcaccctttt ttacaagtcc cctgagagcg tatgtcaccc tcgatttgcc caaagcatgg
     3781 ttgagcctgc caagcgttgc atcatcccca agctggacaa tcttttcaaa cagggttttt
     3841 tctgatggcg aaaactcgca ttcacccaaa atttcaacct ttgtctcaat ccttggcgat
     3901 ggtggcatcc agaggttatc ggagagcgtc gtgccagcct ttgaaaacgc aagtgaatct
     3961 gcaacaatgg tcgcctctat gccctcctca tcaataacgg gtgtgtcgta aacaagcgag
     4021 accatctctt tcagttcgat cccggcctct tcatcaccaa tacccaccac cacacccttt
     4081 gccgtcctgt tccttaacgg gacaagaacg aggcaacctg gtaccactct gcctgaaaga
     4141 gactcaggga ctgaataggt cagctcaagc ccctcgacca taacatgcac gttcacacct
     4201 aaaggctaat gccccaaaat tcccttttca aggcattaat tttctataca ctctggctta
     4261 tgttttgttt cgtgatttca tcgtggcaaa g
//
LOCUS       DAOSRI010000150         4274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18643_length_4274_cov_3.381844, whole genome shotgun sequence.
ACCESSION   DAOSRI010000150 DAOSRI010000000
VERSION     DAOSRI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18643_length_4274_cov_3.381844"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK717_05290"
     CDS             complement(<1..541)
                     /locus_tag="PK717_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOR47111.1"
                     /translation="MRRVVVTGLGLVTPAGIGKEAFWSSLVEGKSGVGKISKYDMSGY
                     PVQIGAEVKDFDPSKYIDSKEIPRTDLFVQYALAAAMLCVQDSKINLESSNRDRIGTL
                     ISTGIGGLQTIEKNIMARIEKGPTRVSPLFVPMMIGNMASAHVSMKYGLRGPSSNIVT
                     ACATSNYALAEASEMIKRDD"
     gene            complement(541..783)
                     /gene="acpP"
                     /locus_tag="PK717_05295"
     CDS             complement(541..783)
                     /gene="acpP"
                     /locus_tag="PK717_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR47112.1"
                     /translation="MNTNEIFEQMKDIIVEKLGVEKDKIKMESKFMDDLGADSLGLVD
                     VTMELEEKFGVSIPDEDLPKITTVASAVDYVQKKLS"
     gene            complement(806..1552)
                     /gene="fabG"
                     /locus_tag="PK717_05300"
     CDS             complement(806..1552)
                     /gene="fabG"
                     /locus_tag="PK717_05300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOR47113.1"
                     /translation="MVDLEGKVALVTGSSRGIGRSIALELAKDGADIAVNYPFEAELE
                     NANETARLVHELGRRALVVEANVANLSSCEEMAKRVVSELGKIEILVNNAGVTADTLI
                     LRMKESDWDKVIEINLKGTFNCTKACLRALMETKGRIINISSVIGIMGNIGQANYAAS
                     KGGIIALTKSVAKEMGSRGITCNAIAPGFIETPMTQALTEQVRNEYLSKIPLRRFGLP
                     EDIAGVASFLASPKGSYINGATIVIDGGLL"
     gene            complement(1546..2523)
                     /locus_tag="PK717_05305"
     CDS             complement(1546..2523)
                     /locus_tag="PK717_05305"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOR47114.1"
                     /translation="MINSGIFGIGTYLPEKVLTNFDMEKIVDTTDEWIVSRSGIRNRH
                     VVAEGQATSDISVIAAQRALENAGLEAKDIDMFIVATATPDMLFPSTACIVASKLGIV
                     GPPAFDIAAGCTGFVYALTLANSFIKSGIHKRILVIGAESLTRILDWTDRSTCVLFGD
                     GAGAVIVGETERNGIVHSVLGADGSGGHNLTLPAGGSALPASHETVDKRLHYVHMNGP
                     EVFKFAVRAMAKSCKEVLEKAGMTVSDINWFVPHQANIRIIESASQKLGIPMEKVLVT
                     LDKTGNTSAATIPITIDMAVKEGKIKKGDKLLAVAFGAGLTWGAVVLEW"
     gene            complement(2520..2957)
                     /gene="fabZ"
                     /locus_tag="PK717_05310"
     CDS             complement(2520..2957)
                     /gene="fabZ"
                     /locus_tag="PK717_05310"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOR47115.1"
                     /translation="MELNQSEIQKILPHRYPFLFVDKILELEPGVKAVGVKAVTGTES
                     HFQGHFPNYPVMPGVLIVEALAQVGAVCGLSLPEYEGALAFFAGIENLRFKRQVVPGD
                     LLRLEIVVESVKMGVFKCNATAYVGDEVAVSGRLLAALRKVEK"
     gene            complement(3248..>4274)
                     /locus_tag="PK717_05315"
     CDS             complement(3248..>4274)
                     /locus_tag="PK717_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47116.1"
                     /translation="GSKEPPSKPYNLTCVPGDRAIKLQWTAAFAPPGKVISYYVYRAD
                     ESGQAQKNKSPIHDFPVSLTQYTDIGLTNGKTYYYIVRTIDQFGNISEASNETFCQPK
                     AVHPPTFPQPLPPAGGAPVPGDGSFDNPWVIEQNPWEFDWCGFQPQTLVIIAGTQYVA
                     DGNGCIHVSITLKPGGKNQIEYTIITPTGDTLSGSIWATLKDTNVHIELWIGRGYMMR
                     NNQQMTLDQPPEIKFDRTMIPIRAVSEALDCQVEWDEKQRKITVSNCLNKIELWINKP
                     TAVVNGTPIAIDDKDKKVVPYISKAGRTLVPFRFISETLGANVQYDANEKKVTIDYTP
                     PKCPKNP"
BASE COUNT         1046 a         1062 c          930 g         1236 t
ORIGIN      
        1 cgtcatcgcg cttgatcatt tcggaggctt ccgcgagggc ataattggag gttgcacaag
       61 cggtaacgat gttcgaggaa gggcccctca aaccatattt catggacaca tgggcagaag
      121 ccatatttcc aatcatcatt ggcacaaaca atggggaaac cctggttggg cccttctcga
      181 tccttgccat gatgttcttc tcgatagtct gcagaccacc aataccagtt gatattaatg
      241 tcccaattcg gtccctgttg gaactctcaa ggtttatctt ggaatcctgg acacatagca
      301 tggcagcagc aagagcgtac tggacgaaaa ggtctgttcg cggaatttct tttgagtcaa
      361 tgtatttgga tgggtcaaaa tccttgacct cagcaccaat ctgcaccgga tagcctgaca
      421 tgtcatattt tgaaattttg ccaacgccag acttgccttc aacaagtgaa ctccagaaag
      481 cttcttttcc gataccagcc ggcgtaacca ggccaaggcc agttacgact acgcgtctca
      541 ttacgaaagc ttcttctgga cgtagtcaac agctgatgct actgttgtta ttttcgggag
      601 gtcttcgtcc gggattgaaa cgccaaattt ctcctcaagt tccatggtga catccacaag
      661 gccaaggcta tctgcgccaa ggtcgtccat aaacttggat tccatcttga tcttgtcttt
      721 ttcaacgcca agcttctcga ctatgatgtc tttcatctgt tcaaaaattt cgtttgtgtt
      781 catgattcac ctcttgtaaa gtggattaga ggagcccgcc atcgattacg atggtggccc
      841 catttatata actgcctttc ggggaggcaa ggaaggatgc tactcctgcg atgtcttcgg
      901 gaagaccaaa tcttcgaagt ggtatcttgc tcaaatattc atttctgacc tgctctgtaa
      961 gtgcctgggt cattggtgtt tcaataaaac ctggagcaat tgcgttgcat gttatgcccc
     1021 ttgagcccat ctctttggca acagattttg tcagagcaat tatgccgcct tttgaagcag
     1081 cataatttgc ctggcctatg ttgcccatga ttccaatcac tgaagaaatg ttgatgatcc
     1141 tgccttttgt ttccattagc gccctaaggc aagcttttgt gcaattaaag gtacctttga
     1201 ggttgatttc gattaccttg tcccagtcag actctttcat ccttaaaatc aaagtatctg
     1261 ctgtcactcc tgcgttattg acaagaattt ctattttgcc caactcagaa acaacccttt
     1321 ttgccatttc ttcgcacgat gaaaggttag caacatttgc ttcaacaaca agggctctcc
     1381 tgcccaactc gtggacaagc cttgcggttt cgtttgcatt ttcaagctcg gcctcaaaag
     1441 ggtaattcac tgctatatca gcaccatctt ttgccagttc aagcgcaatt gatctgccta
     1501 tgcctcttga ggaaccggtt accagtgcta cttttccttc aagatctacc attccaggac
     1561 cacagctccc catgtcaatc cagcgccaaa agccactgca aggagcttgt ctcctttctt
     1621 tatttttcct tcttttactg ccatgtctat ggttatcgga attgtcgctg cagaggtatt
     1681 accagtcttg tccagggtca caaggacttt ttccatcggt atcccaagtt tctgggaggc
     1741 tgactcgatg atccttatat ttgcctgatg tggcacaaac caattgatgt ctgaaactgt
     1801 cattccagcc ttttccaaaa cttctttgca gctctttgcc attgccctta cggcaaactt
     1861 gaaaacttca ggcccattca tgtgaacata atgcaatctt ttatcaacgg tttcatggga
     1921 ggcgggcagt gccgaacctc cagcaggaag ggtgaggttg tgcccaccag aaccatcggc
     1981 cccaaggacc gagtgcacaa tgccatttct ttcagtttca ccaacaatga ctgctccagc
     2041 accatcacca aaaagaacgc atgttgatct gtcagtccag tccaaaattc ttgtgagcga
     2101 ctctgcccca ataacaagga ttctcttgtg gattccagac ttaataaatg agtttgcaag
     2161 agtcaaagca tagacaaaac cggtgcaacc agctgcgatg tcaaatgcag gtgggcctac
     2221 aatgcccaat ttggaagcaa caatgcaagc agttgacgga aacagcatgt caggcgttgc
     2281 tgtggcaacg ataaacatgt ctatgtcctt cgcctcaaga cctgcatttt caagggccct
     2341 ctgggcagca attacggaaa tgtccgatgt tgcctgtccc tcggccacaa catggcgatt
     2401 tcttattcca gacctagaca ctatccattc atcggtagtg tcaactattt tctccatgtc
     2461 aaaatttgtc agtaccttct cgggtaggta ggtccctata ccaaagattc ctgaatttat
     2521 catttctcca cctttcgaag tgcggccaga agcctccctg aaacagcgac ttcatcacca
     2581 acataggctg ttgcattgca cttgaaaaca cccatcttga cactctcaac aacaatctca
     2641 agcctcaaaa gatcaccagg aacaacctgt cttttgaacc tcaggttttc aataccggca
     2701 aaaaaggcca acgcaccctc gtattcgggg agggaaagcc cacaaacagc gccgacctgg
     2761 gccagggcct caacaatcaa tacgccgggc attacgggat agttagggaa atgcccctga
     2821 aaatgggact ctgttccagt taccgccttg actcctacag cctttacacc aggctcaagc
     2881 tcaagtattt tgtccacaaa aaggaacgga tagcggtgcg gtagaatttt ctgaatttca
     2941 ctttgattaa gctccacttt gcctcctgtt ggcaaacatg agcagaagcg acgccaaaaa
     3001 cgctgcaaaa aggaaatagc ttgcgacggc tgttgtcttt acgactttct gcttgttttg
     3061 tctgtcacgc catgcatcaa taacgctctg cgtgaacaat ccgggcaccc tgtgaacctt
     3121 aagcaaattt tttaagtcac tcatcatatt gttactgtct ttgattcacg tttgccctgg
     3181 ctggttacat taaaaaatcc cccacccgta tgggtggggg attaaattca gctctgaaat
     3241 tgtcttttta cgggttcttg ggacactttg gtggtgtata atctattgtt accttttttt
     3301 cgtttgcatc atactgaaca tttgcaccaa gcgtttcgct tatgaagcgg aacggaacaa
     3361 gggtcctgcc agctttagat atatatggaa caactttctt atccttgtca tctatcgcta
     3421 taggagtccc atttacaaca gctgttggct tattgatcca aagctcgatt ttattaaggc
     3481 agttgctgac agtaattttt ctctgttttt catcccattc cacctggcag tcaagcgcct
     3541 cactgacagc ccttataggg atcattgttc tgtcgaactt tatttctggc ggctggtcaa
     3601 gtgtcatctg ctggttgttt ctcatcatat agcccctacc aatccagagt tctatatgaa
     3661 catttgtgtc ttttagtgtt gcccagatgc ttccagagag cgtgtcacca gtcggggtta
     3721 tgattgtgta ttcaatctgg ttctttccgc ctggtttaag tgttattgaa acatgaatgc
     3781 aaccattgcc atctgcaacg tactgggttc cggctatgat gaccagcgtt tgcggctgga
     3841 agccacacca gtcaaattcc caaggatttt gctctattac ccaaggatta tcaaatgagc
     3901 cgtcgcctgg tacaggcgct ccaccggccg gtggaagagg ctgcgggaag gtaggtggat
     3961 ggacggcctt tggctggcag aaggtctcat ttgaggcttc cgatatgttg ccaaactggt
     4021 caatcgtcct gacgatgtag tagtatgtct tcccattggt caaacctatg tcagtgtact
     4081 gcgttagaga aactgggaaa tcatgtattg gcgacttgtt cttttgggcc tgacctgatt
     4141 catcggctct gtagacataa taggaaatga cttttcctgg tggcgcaaaa gcggctgtcc
     4201 actggagctt gatggccctg tcgccgggca cgcaggtgag gttgtatggt tttgaaggtg
     4261 gctcttttga cccg
//
LOCUS       DAOSRI010000151         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18743_length_4251_cov_4.265253, whole genome shotgun sequence.
ACCESSION   DAOSRI010000151 DAOSRI010000000
VERSION     DAOSRI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18743_length_4251_cov_4.265253"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2339
                     /locus_tag="PK717_05320"
     CDS             177..2339
                     /locus_tag="PK717_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47117.1"
                     /translation="MDKLESELGSDSIIKLRLHSNPNDEFYIEEVDKRGQMYERKSYP
                     TIAFNGNFIKRSGMTEKEVEDTVSKVRSSPLSFNISLNGKLKEEAAGQKIDFEAMVEQ
                     FGPLDQATLRYTFFLFEDNVDREDQYFQHVVHAIKPSAKGSTMRIKNGETKKLTASFD
                     LKEDWTKEELGAAVAIFDQDTFEVYAYGAWKIKAPHLTSPLPGKVSPNSPLSLPFDKD
                     MDPATFNEGTVLLVDQNGLAVPGKASVSGSKCTFAPNKAFEEGKKYTLYAKSGQSGIK
                     SKSGEKLIGNIIWKFECEKVAEEPKLEVAEKSFDLGEISTTITKKLAFKNVGTGKLSA
                     TIKSSESWLRTENSEFEIEPDGSFETIMTVDPLGLEPGPYSGKLSIGSNGGNSEIAVN
                     FTYKKSGMILKVEPSELDFGKIPKNTKKSLQIEIIEQAGEGGMLEGTITASAPWIKVD
                     PSSFKCDRCKINITLEPNTEGALTGEVTIDSNAGKIVVPVKVLVESAALLDLSVNGSS
                     GTPLRTTSPSYTIPISTTKGATVMVGGKPATVDQNGKANYSVILIPGLNKFEISATLG
                     SQNKKITVDVIYAVTIELWLDKTDITINGLKVTLKTAPTSSSPPLPKELGGSTYMPIR
                     EVAEALGAQVGYDGAEKKVTLTQSLWNGKNIVELWIGKKTAKINGKEVFIDSKAQKLY
                     PTIISAKTMLPLRFVGEALGAGVAWEGATRKITLTYPK"
     gene            2336..3085
                     /locus_tag="PK717_05325"
     CDS             2336..3085
                     /locus_tag="PK717_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOR47118.1"
                     /translation="MKIDFAILGGGGVEPWFANEGVPNKGMLSFAGKPSIEYVIDALR
                     KASLTNRIILVGNGYPDSIKSKVDLMLEEGDSQKANIDRLVDASETEYLVVTTNDIPL
                     VTEKTFDMVVSALQKTGCDLGLPIITKEDTLRMFPGTKRTFLRIKEGNVKIGNLFYIS
                     KTAYKKVEPIVEETSKRRKSAIKQALQLGVPFLLRLLLFKNVEIPELEKRISKMLGVT
                     IKAPILQCPELGVDVDKIEDLEFCRSILEAK"
     gene            3085..3894
                     /locus_tag="PK717_05330"
     CDS             3085..3894
                     /locus_tag="PK717_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOR47119.1"
                     /translation="MGIIEALVLGFVQGLTEFIPVSSSAHLVLVPAFLGWGKPPMWFD
                     VFLHAGTLLGIIVFFWPEVVKYVKAFFAGLPKIGRGLPPDSIFSYNLILSVVPAIIFG
                     LLFESRIESVFQDPKTTSYLLLVTAAFLIVASLMSGKRKLEETTWIDALTVGCAQAIA
                     MFPGISRSGATITAARLLGFGKKSSSKLAFLVAIPAVGAATAKTLYDVYKMVASSGMQ
                     IDWMPSIVGFVVAAVVGYLSFFLFFKVIQKFNFYIFAAYCVALFVVAQIIL"
     gene            3891..>4251
                     /locus_tag="PK717_05335"
     CDS             3891..>4251
                     /locus_tag="PK717_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47120.1"
                     /translation="MSLSPVRTLILGPAMAELERGCDDRAVFGGFSKFVLAKLDQWAK
                     DEPSQNASIKRAIEVFSSYTSLDITSREKAVNDLLKFARGHYVRQKPVPRQQKIHLGT
                     EMSLASIPGIGPERAKLL"
BASE COUNT         1225 a          990 c         1014 g         1022 t
ORIGIN      
        1 gagctgtgaa aaaactgtca gtaattgttc tgctttctct catactttca cagaccgtcc
       61 aggtctactc gaggacaatg ccgctctccg tttcagattt cactccgaaa cgtgcagcat
      121 tctgcgaaat ttttgtggag ccgaactgaa cgaagtgtca cactgccgtg cgcagtatgg
      181 acaaactcga gtctgaactt ggctcggact ctattatcaa attaaggctg cattcaaacc
      241 ccaacgacga gttttacatt gaagaagtcg acaaaagggg gcagatgtac gaaagaaagt
      301 cttatccgac gatagctttt aatggcaatt tcataaagag gtcgggtatg actgaaaagg
      361 aagttgagga cacggtttca aaggtccgct cttcaccctt gagcttcaat atttcgctga
      421 atgggaaact gaaggaagaa gccgcagggc aaaagatcga ttttgaggcc atggttgagc
      481 agtttggtcc tctcgaccag gcaacactca gatatacctt cttcctcttt gaagacaatg
      541 tggacaggga agaccagtat ttccaacatg tcgtgcatgc aatcaagccc tctgcaaagg
      601 gttcaaccat gcgcatcaaa aatggcgaaa ccaagaagct cacagccagt tttgatctca
      661 aagaagactg gacaaaagaa gaattaggtg ccgcggttgc gatattcgat caggacacat
      721 tcgaggttta tgcctatggc gcatggaaaa tcaaagcacc gcatctcacc agtccgcttc
      781 ctggcaaggt atcgccaaac tcgccacttt cgctaccatt tgacaaggat atggaccctg
      841 caacattcaa cgagggcacg gtgctcctgg ttgaccagaa tggcctcgct gttcctggaa
      901 aagcgtctgt ctcagggtca aagtgcacgt ttgctccaaa caaggctttt gaagagggca
      961 agaagtatac gctctacgca aaaagcggcc agtccggcat aaaatcaaaa tcaggcgaaa
     1021 aactgattgg caacatcatc tggaagtttg aatgcgaaaa agtggccgaa gagccaaagc
     1081 ttgaagtcgc agaaaaatcg tttgacctcg gtgagatttc gacaaccata accaaaaagc
     1141 tcgctttcaa aaacgttgga accggaaaac tttcagcgac catcaagtct tcagagtcat
     1201 ggctcaggac tgaaaattcg gaattcgaga tcgagccaga tggttctttt gaaacaatca
     1261 tgaccgttga cccacttggt ctcgaaccgg gaccatattc agggaagctg tcgattggaa
     1321 gcaacggcgg aaattcagaa attgcggtaa atttcaccta caaaaaatcc ggaatgattc
     1381 tgaaggtgga gccttcagag ctggattttg gcaaaatacc aaagaacaca aaaaaatcac
     1441 tccagatcga aatcatcgag caggctggcg aaggcggaat gctcgaggga acaattactg
     1501 cgagcgctcc atggataaaa gttgatcctt catcattcaa gtgtgacaga tgcaagataa
     1561 acatcacgtt agagccgaac acggaaggtg ccttgacagg cgaagtgaca attgactcca
     1621 acgcaggaaa aattgttgtt cctgttaaag tattggtcga gtctgcagca cttcttgatc
     1681 tctcggtcaa tggttcctct ggtactccat tgagaaccac ttctccaagc tacacaatcc
     1741 ctatctccac aacaaaggga gcaacagtga tggttggtgg caagccggca accgtggatc
     1801 agaatggaaa ggctaactac tcagtaatac ttatcccagg actcaacaag ttcgagatct
     1861 cggcgacact gggcagccag aacaaaaaaa taacggtcga tgtaatttat gccgtcacaa
     1921 tagaattatg gctggacaaa acagacatca ctataaacgg cctgaaggtg acactcaaaa
     1981 ctgctccaac atcttcctcg ccaccgcttc cgaaagaact tggcgggagc acatacatgc
     2041 caataaggga agtcgcagag gcccttggag cacaggttgg ctatgatggt gcagaaaaaa
     2101 aggtcaccct gacgcaatcg ctctggaatg ggaaaaacat cgttgagttg tggattggca
     2161 agaaaaccgc caagataaat ggcaaagagg tcttcatcga ctcaaaggcc caaaagctct
     2221 atccaacaat cattagcgca aaaaccatgt taccgctaag atttgtcgga gaggctcttg
     2281 gggcaggtgt ggcctgggaa ggcgccacaa gaaagataac gctgacatat ccaaaatgaa
     2341 aatcgatttt gcaatattag gcggcggcgg agtagagccg tggttcgcaa atgagggcgt
     2401 gcccaacaag ggcatgcttt catttgcagg aaaaccatcg attgagtacg tcatcgatgc
     2461 gcttcgcaag gcttccctca cgaacaggat catccttgtt ggcaacggct atccggattc
     2521 cataaaatca aaagtggacc tgatgctgga agagggcgac agccagaagg caaacataga
     2581 tcgccttgtg gatgcctcag agacagagta tctcgttgtg acaacaaacg acatcccgct
     2641 ggtaaccgaa aaaacctttg acatggtggt ttctgcactc cagaaaacag ggtgtgatct
     2701 tggtttgcca atcataacca aagaagacac cctgaggatg ttccccggaa caaagcgcac
     2761 ttttctccgc atcaaagagg gaaatgtcaa aatcggaaac ctcttctaca tcagcaaaac
     2821 cgcctacaaa aaggtggagc caatagtcga ggaaacatca aagaggagaa agtccgccat
     2881 aaaacaggcc ctccagcttg gtgtgccgtt ccttctgaga ttgcttttgt tcaaaaatgt
     2941 cgaaataccc gagcttgaaa agagaatttc aaaaatgctt ggcgttacca taaaagcacc
     3001 aatattgcag tgcccggagc tgggcgtcga tgttgataaa atagaggacc tcgaattctg
     3061 caggtccatt ctggaggcaa aatagtggga atcattgaag cattggtcct cggctttgtc
     3121 cagggtctca cagagtttat tccggtctcg tcttctgccc atcttgtcct tgttcccgct
     3181 tttttggggt ggggcaaacc accgatgtgg ttcgatgtgt tccttcatgc tggaacgctc
     3241 cttggaataa tcgtcttttt ctggccggaa gtagtcaaat atgtaaaggc cttttttgct
     3301 ggtttgccaa agattggcag gggtctcccg ccagattcga ttttttcata caacctcata
     3361 ctttcagttg taccggcaat catcttcggc ctgctctttg aaagcaggat tgaatctgtt
     3421 ttccaggacc caaaaactac ctcttacctt ttgcttgtta ctgcagcttt cctcatagtt
     3481 gcttctctca tgagtggcaa acgcaaactt gaagaaacaa catggatcga tgcactaact
     3541 gttggctgtg cccaggcgat tgccatgttt cctggcatat caaggtcagg cgccacaata
     3601 accgcagcca ggctgttggg gtttggcaaa aaatcatctt ccaaactggc ttttcttgtt
     3661 gcaattcctg cagtcggcgc tgcaacagca aaaacactct atgatgttta taaaatggtg
     3721 gccagttcag ggatgcagat tgactggatg ccttccatag ttggctttgt ggtggccgcc
     3781 gttgttggct acttatcatt tttcctgttc ttcaaggtca tccagaaatt caatttctac
     3841 atctttgcgg cctactgcgt tgccctcttt gttgtagccc agattatctt atgagtcttt
     3901 ctcctgtcag aacgctcatc cttggccctg caatggccga gcttgagagg ggttgcgatg
     3961 acagggcggt gtttggcggg ttttcaaaat ttgttctggc aaagctcgac cagtgggcaa
     4021 aagacgaacc cagtcaaaat gccagcataa aaagggctat tgaggttttt tccagttata
     4081 cctccctgga tatcacatca agggaaaagg ccgtaaatga cctgttaaag tttgccaggg
     4141 gccattatgt caggcagaaa ccggtaccca ggcaacaaaa aatacatctg ggtacagaaa
     4201 tgtctctcgc ttccattcca ggaatcggcc ccgagagggc aaaactctta a
//
LOCUS       DAOSRI010000152         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_18933_length_4223_cov_3.802303, whole genome shotgun sequence.
ACCESSION   DAOSRI010000152 DAOSRI010000000
VERSION     DAOSRI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18933_length_4223_cov_3.802303"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PK717_05340"
     CDS             <1..1816
                     /locus_tag="PK717_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47121.1"
                     /translation="IQPGGVMIEFTKASKIRNPEEPGSYAIKFTSQGSDGVLTTDEYS
                     IIIKRITIDNVLVKPINAKAIATWVIEGSLAFTGDLDTGSSFTLTFPNTTIMPKDIPP
                     DSITINGVQVQSATGSGNVLTIQIPSKIQSGGVFKIVISPTSGIVSPEVSSTDYKITM
                     VTSKDQQGGESVSFFIAPPIPETTLLIKPPQPDGKNGWFVHPPSIDFVCTSKTATIKV
                     WWNWKKDQYIKWEPGNWSPLADAQRIDTLYWYAEDTYGTEELKSYDFKIDTIAPTFNI
                     TYPVGDTVLTRDNTYKITGIGDSTELAMYEDTEKLRFVVPNILINGKQVPIVQPDPKG
                     DTTKVPNAGYFESIVNLNEGKNVFTVVAEDDAGNSQTKQVTIIKDTIAPQASIIKPGP
                     AESSNCNTIEIQGKTEPEALVSVGSQIIQVEPDGMFYYDYQPKKFGANEVSFEVVDQA
                     GNKTTLKYTIWWGTEARLWVTGDGKQPNINGAEPNPPLPTTPYVSKGNTLVPLRYLAE
                     KMMGGTVDYKAGLVTITLPNGTIITHTIGKNTFTKTEKGKKPIIVTMPIASENNKGSV
                     FLPMRKFIEDGLGQKVEWNNALKQITVIIPPDSTKCKK"
     gene            complement(1824..2171)
                     /gene="acpS"
                     /locus_tag="PK717_05345"
     CDS             complement(1824..2171)
                     /gene="acpS"
                     /locus_tag="PK717_05345"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOR47122.1"
                     /translation="MSTIKGIGFDLVDCDRFRAMAQKRPTLIERLFSPEEIAYANKYS
                     DPTVHLCACFAVKEAYLKALGTGINGQVLKNIEMGHEKTGTPFVTISGERVPALVSVS
                     HTDKTAGAVVILF"
     gene            2265..2684
                     /locus_tag="PK717_05350"
     CDS             2265..2684
                     /locus_tag="PK717_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOR47123.1"
                     /translation="MIGSTKVLELYICDDCAKKMLESRRAQATFGQKGVSDWLSSVVA
                     QAREQKDIDLACPNCGKKYLAFVKDNYIGCPACYIAFKERLGAIINEFSLSRESTESL
                     KSDLERAIQEERFEDAAKIRDYLKSQSQKSKNIQPSD"
     gene            2677..3333
                     /locus_tag="PK717_05355"
     CDS             2677..3333
                     /locus_tag="PK717_05355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR47124.1"
                     /translation="MTDRSERLDLFDKWSARYDDCTGNNEFPFIGFRDTLLSVLNHSN
                     IGEECSVLELGIGTGALAVLYADIARNVFGIDFSPKMAEICRANVPGARIFQADVTKP
                     FDFLDGQKFDRVVSNYLFHEFCDETKLQIIEKCFNYHIKEAGFMVIGDISFENRIKLD
                     ECKNRYLDLWDDDEFYWDANILVKRISDLGYLVFYSQISECAGIYVIKRKRAQGDSNP
                     "
     gene            complement(3307..3380)
                     /locus_tag="PK717_05360"
     tRNA            complement(3307..3380)
                     /locus_tag="PK717_05360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3344..3346),aa:Arg,seq:ccg)
     gene            complement(3426..>4223)
                     /locus_tag="PK717_05365"
     CDS             complement(3426..>4223)
                     /locus_tag="PK717_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47125.1"
                     /translation="VKVEATPIKVSTNGKNYTMGNTAFLDPQKPLKLVNDSDKTWKKI
                     KIRFTFSAKEFKLDIKTSEKGIKVNSGSPFKLEAKIENTTPVVGFYWSWDSKNWNKLD
                     KEGATIETGLGGAPSPGKKTINLKTVALFKDTTQEVTKTFEIELLPTNEFKNQPVFQD
                     PPNQLEGSSWKLQSFKIPKGERYLMIKTDSQSPGLDLDVFLYLDKNGDGKPTEDEQIS
                     VSNGPTCQERILINNPTEGPYILLCQGCTCPTENSTFDVKLSCIPIW"
BASE COUNT         1183 a          920 c          975 g         1145 t
ORIGIN      
        1 aatccagccg ggcggagtaa tgattgaatt cacgaaagcc tcgaagataa gaaatcctga
       61 agaaccaggt tcttatgcga tcaagtttac gtcacaaggt tctgatggtg tcctcacaac
      121 agacgaatac tcaatcatca tcaaacgcat caccattgac aatgttttag tgaaaccaat
      181 caatgcaaaa gccatagcta cctgggtcat tgaggggagc cttgccttta ctggtgacct
      241 tgatactgga tcatcgttta cacttacttt cccaaataca accattatgc cgaaggacat
      301 ccctccggac agtataacta tcaatggtgt gcaggtgcag tctgcaactg gctctggtaa
      361 tgtgctcaca atccaaatac catcaaagat ccagagtggt ggagtcttca aaattgttat
      421 cagtccaact tctggaattg tgtccccgga agttagctcg acagactaca aaatcactat
      481 ggtaactagt aaggatcaac agggcgggga gtcggtgtct ttctttatcg ctcctccaat
      541 ccctgaaaca acacttttaa taaagccgcc acagccagac ggcaaaaatg gctggtttgt
      601 ccatccgccg tcaattgatt ttgtttgcac ttccaaaacc gcaacaataa aggtctggtg
      661 gaactggaag aaagaccagt acatcaagtg ggagcctgga aattggtcac cgcttgccga
      721 tgcacagcgc atcgacacct tgtattggta tgctgaggac acctatggga cagaagagct
      781 caaatcttac gatttcaaga ttgatacaat tgccccgacg ttcaacataa cctatccagt
      841 tggcgacact gtcctcacaa gggacaatac ctacaagatt acaggcattg gcgattcaac
      901 tgaacttgca atgtatgagg acactgaaaa gctccgcttt gttgttccaa acatacttat
      961 caatggcaag caggtcccaa ttgtccagcc tgacccaaaa ggtgacacaa cgaaggttcc
     1021 gaatgccggc tactttgaaa gtattgttaa tctcaatgag ggcaaaaatg tcttcacggt
     1081 cgtcgcggaa gatgatgcgg gtaacagcca gacaaaacag gtcaccatca ttaaggatac
     1141 aattgcacca caggccagca taatcaagcc aggcccggca gaatcctcaa actgcaatac
     1201 gattgaaatc cagggcaaaa cagagccaga agcacttgtt tctgttggct cccagataat
     1261 acaggttgag cctgatggca tgttctacta tgattatcag ccaaagaagt ttggtgccaa
     1321 cgaagtatca tttgaagttg ttgaccaggc tggcaacaag acaacgctca agtatacaat
     1381 ctggtggggc actgaagcca gactctgggt cactggtgat ggcaaacagc caaatatcaa
     1441 cggagcagaa ccaaatccac cacttcccac aaccccgtat gtttcaaaag gtaatacgct
     1501 tgtcccatta agatatcttg cagaaaagat gatgggtggc acagtagatt acaaagcggg
     1561 attggtaacg atcacattgc caaatggaac cattattact catactattg gcaaaaatac
     1621 cttcaccaag acggaaaaag ggaaaaaacc tattatagtt acaatgccaa tagcatcgga
     1681 gaataacaag ggttcagttt tccttccaat gagaaagttc attgaggatg gacttgggca
     1741 gaaagtcgaa tggaacaatg cactcaagca gataacagta attattccac ctgattcaac
     1801 aaagtgcaag aaatagcgac aatctagaag agtatcacga cagcaccggc agtcttgtcg
     1861 gtgtggctta ctgaaacgag cgccgggacc ctctcaccag aaatggtgac aaagggggtc
     1921 ccggtttttt catgtcccat ctctatattt tttagcacct ggccattgat tccagtccca
     1981 agcgctttta ggtaggcctc cttgaccgca aagcatgcgc aaaggtggac cgtcgggtcc
     2041 gaatatttgt tcgcataggc aatttcttcc ggagaaaaaa gcctttcaat cagggtcggc
     2101 cttttttggg ccatggccct gaatctgtca caatctacta ggtcaaaacc tattccttta
     2161 atggtggaca tctgattccc ctatattata attggttttg aaaggaagtg taaatgcttt
     2221 gtcagatttg caaacaaaag gaagcgactg ggcactatgt tcaggtgata ggctcaacga
     2281 aagtccttga attatatata tgcgatgatt gcgcaaaaaa aatgctggag tcaaggaggg
     2341 cccaggccac ttttggccaa aagggtgttt ctgactggct ctcaagcgtg gttgcacagg
     2401 caagggagca gaaggatatt gatctggctt gccccaattg tggcaaaaaa taccttgcct
     2461 ttgtaaaaga caattatatt ggttgcccgg cttgctacat tgccttcaag gagaggcttg
     2521 gtgcgattat aaatgaattc tcgctctcca gggaaagtac cgaatcgctc aaaagtgacc
     2581 ttgaaagggc catccaagaa gagcgttttg aagacgctgc aaaaataagg gattatctaa
     2641 aatcccagtc ccaaaaatcc aaaaatattc agccaagtga ctgatcggtc agaaagactg
     2701 gatctttttg acaagtggtc ggcccgatat gatgactgca cgggaaacaa tgaattcccc
     2761 tttattggtt tcagggacac acttctttct gttctaaacc attcaaatat tggcgaggaa
     2821 tgttctgtgt tggaactcgg cattggtacg ggagcacttg ccgtgttgta tgcggatatt
     2881 gcaagaaacg tttttgggat tgatttctcg ccaaaaatgg ctgaaatatg cagagctaat
     2941 gttccaggag ccaggatatt ccaagccgat gtcacaaaac catttgattt tctggatggg
     3001 cagaaatttg acagagtagt ttcaaattat ctatttcatg aattttgtga cgaaacaaag
     3061 ctccagataa tcgaaaaatg ttttaattat cacataaaag aagcgggatt catggttatt
     3121 ggtgacatct catttgaaaa ccgcatcaaa cttgacgaat gcaaaaacag ataccttgat
     3181 ctttgggacg atgacgaatt ttactgggat gcaaatattc ttgtaaaaag aatttctgat
     3241 ttgggctatc tggtgttcta ttctcaaatt tccgaatgcg ctgggatata tgtcataaaa
     3301 agaaagcgcg cccaagggga ttcgaacccc taacctgcgg ctccggaggc caccgctctg
     3361 tccagttgag ctatgggcgc gtgccggatt atgttaaaat ccggcacgct tgttgtcaac
     3421 cggttttacc agattgggat acaggaaagt ttcacatcaa atgtagaatt ttcagtgggg
     3481 catgtgcatc cctgacaaag aagtatgtat gggccttcgg ttggattgtt tatgaggatt
     3541 ctctcctggc aggttggccc gtttgagaca ctgatttgct cgtcctccgt cggtttgcca
     3601 tcgccgtttt tgtccagata aaggaacacg tcaagatcaa gccctggtga ctggctgtct
     3661 gtttttatca tcaggtacct ttcgcccttt ggaatcttga atgactggag cttccacgaa
     3721 gagccttcaa gctggtttgg cgggtcctgg aacacaggct ggtttttgaa ttcgtttgtt
     3781 gggagaagct caatctcgaa cgtctttgtt acctcctggg tcgtgtcctt gaaaagcgcc
     3841 acggttttga ggtttattgt tttcttgcct gggcttggtg caccacccag cccagtttcg
     3901 attgttgcgc cttctttgtc tagtttgttc cagtttttgc tgtcccatga ccagtagaag
     3961 ccaacaactg gcgttgtgtt ttcaattttt gcttcaagct taaacgggct gccgctgttt
     4021 acttttatcc ctttctctga cgttttgatg tccagcttga attcttttgc cgagaaggtg
     4081 aagcggattt tgattttttt ccaggtttta tctgaatcgt ttaccagctt caggggtttt
     4141 tgcgggtcga gaaatgctgt gttgcccatg gtgtagtttt tgccatttgt ggaaaccttt
     4201 atcggggtcg cttcgacttt gac
//
LOCUS       DAOSRI010000153         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19024_length_4206_cov_3.420381, whole genome shotgun sequence.
ACCESSION   DAOSRI010000153 DAOSRI010000000
VERSION     DAOSRI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19024_length_4206_cov_3.420381"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PK717_05370"
     CDS             complement(<1..1378)
                     /locus_tag="PK717_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47126.1"
                     /translation="MPEYIHEEEALGKAYDSRLIKRLLKYLKPYVGKTILSLLMLVFW
                     TALRLASPIIMKLALDKYISKGDIKGLGILMLAMFTLIIFQSIVRYYQILIMSFIGQY
                     LMRDMRRQIFDKLQDIQMSFFDHNPVGRLITRITSDVDAIYEFLTQGIITLITDVTIV
                     IGSTIMLFILQPTLALIAVSIMPPMMVAAVVFKNLATKFYREVRVKTAKVNAFLQESI
                     TGVRTIQWSGREDINKGMFAKISGELKGSQLKAVLNYTVFFQAIDLFETVATGFVVWY
                     GSLLVLKVAITVGLLVAFTSYLKEFYASMYDISDHFNTMQEAMTSSERIFKLLDEPVV
                     IKEKVDAIWPEEPINGHVEFQDVQLEYQPGQPVLKGVSFEVKPGQKVAVVGPTGAGKS
                     SIISLLSRLYDIQGGDIKIDGHSIYNLKLGYLRSQISVVLQDPFIFSGPIIDNITLLS
                     PISRERARE"
     gene            complement(1371..3125)
                     /locus_tag="PK717_05375"
     CDS             complement(1371..3125)
                     /locus_tag="PK717_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47127.1"
                     /translation="MTTFQYYSRLLKPYRWKYVLAMIGVLVNTSDMLVPMFISTSIDA
                     AYLKKPDAKYIILSALLWVVGIAVVRSFTFVASRYTLVGVSRHTAYDLRNTIYDKILR
                     LPDKFFVGMSTGEIMNRASSDVQSITMFLGMGGNLFPNAVLRTVLGTILMISISWKLA
                     IPILLFVPVLFFIEYLFGKKIFKLYKILQDYSDKVVSRVQENYSGARTVRSYNQGDHE
                     KRMYKGMMDKYVQTIKPLNIMDALFWPVLNMASWMPILVILWYGGVLYMKGQLTLGGL
                     SAFIAYVSLMIWPIINLGWVTNLYQRSNASLKRIVEITEVVPDIKSPQNPYKPETIEG
                     NIELKDVKLSYGTNINSIDGISITIPKGQVVGVVGPVGSGKSSFGKLLLRVWDSDSGE
                     VSIDGVDIKKWDLKMLRKSFGYVPQDNFLFSTSIGENIAFAKPDATKEEIEKVAEMVQ
                     IKDEIEKFPQSFDTVVGERGVTLSGGQRQRVAIARALLLDPPMMLLDDPLSAVDTSTE
                     DAIIDTLKPIIKGRTTIIIAHRVSAMRLADRIVVLDKGRIVEDGTHQELMTKGGYYAD
                     LVEKQRLATQIGQEGIDA"
     gene            3225..3884
                     /locus_tag="PK717_05380"
     CDS             3225..3884
                     /locus_tag="PK717_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOR47128.1"
                     /translation="MAGFWQRISDILQSNVNDWLTKMEDPEKMLPLKIDQMKQDLVEA
                     TDAAAESMAVSKQTEMRLADIKFDLKKYNDAAQQALSASKEELAKKCMARVLTLEKQQ
                     KSAEESHVRQVELNAKLKDVIAALKAKIEEAEVKMDELIARNKAAKAQEKVVKTMGNI
                     NVADIGTTFNELEQRVIKKENKVAALAELGSAADSLDDEIASINEEDEINKRLQQLKG
                     K"
     gene            3884..>4206
                     /locus_tag="PK717_05385"
     CDS             3884..>4206
                     /locus_tag="PK717_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47129.1"
                     /translation="MNVLLFLLGAVVAGILLGVILYFTKKSVMDRIQKEKRSAMGDLD
                     KRYFAISDDLKLIQDMGDLLSGKYKNATPEKVARIKELASSFESGWIARRKMVQDPSN
                     QTEATT"
BASE COUNT         1247 a          930 c          905 g         1124 t
ORIGIN      
        1 cttctctggc tctttccctg ctgataggcg agaggagagt tatattgtca atgattgggc
       61 ctgaaaagat gaatgggtcc tgaagcacaa cagaaatctg gctcctcaga tagcctagtt
      121 tgaggttgta tattgagtgg ccatctatct ttatgtcccc gccctggatg tcatacagtc
      181 ttgagagaag actgattata ctggacttac cggcaccagt tggcccgaca acggccactt
      241 tctggcctgg ttttacttca aacgacacgc ccttaaggac aggctgaccg ggctgatatt
      301 ccagttgtac atcctgaaat tcaacatggc cattgattgg ttcttccggc cagatggcat
      361 caactttttc ttttatgaca acgggttcat caagaagctt gaatattctc tctgaagagg
      421 tcatcgcttc ctgcatggtg ttgaaatggt cagatatatc atacatcgag gcatagaatt
      481 ccttcaggta cgatgtaaaa gccacaagaa gtccgacagt tattgccacc ttgagtacca
      541 gtaagcttcc ataccagaca acaaaacccg ttgcaactgt ttcgaacagg tcaattgcct
      601 ggaagaaaac tgtatagtta agaacagcct tgagctggct gcctttgagc tctccggaaa
      661 ttttggcaaa catgcctttg tttatgtctt ccctgccact ccactggata gtacgaacac
      721 cagtgatgct ctcctgtagg aaagcattga cctttgctgt tttgaccctg acctccctgt
      781 aaaacttggt tgccagattc ttgaatacca ctgccgcaac catcattggg ggcataatcg
      841 aaacagcgat aagcgcaagt gttggctgga ggatgaagag cattattgta gaaccgataa
      901 cgatcgtcac gtctgttatg agggttatta tgccctgagt cagaaactcg taaatcgcgt
      961 caacatctga tgttattctg gtgataagcc ttccaactgg attgtggtca aaaaatgaca
     1021 tctgaatgtc ttgcaatttg tcaaaaatct ggcgcctcat gtccctcatg agatactgac
     1081 caatgaaaga catgataagg atctgataat acctgacaat ggactggaag ataatcaggg
     1141 taaacatcgc aagcataaga attcctaggc ctttgatgtc ccctttgcta atgtatttat
     1201 caagagcaag cttcataatt atcggcgaag caagcctcag ggccgtccag aaaacgagca
     1261 ttagcaaaga aagaattgtt ttgccgacgt atggcttcag atattttaga agtcttttta
     1321 tgagccttga atcataagct tttccaaggg cttcttcttc gtgtatatac tcaggcatcg
     1381 atgccctcct ggcctatctg agtggccagt ctctgttttt caacaagatc agcgtaataa
     1441 ccgccttttg tcatcaactc ctggtgagtg ccatcctcca caatccttcc cttgtcaaga
     1501 acaacgatcc tatcggcaag cctcattgcg cttaccctgt gggcgattat tattgttgtt
     1561 ctgcccttta ttatcggttt aagcgtatca atgattgcgt cctcagtcga agtatcgacc
     1621 gcagacaaag gatcatccag aagcatcatc ggtggatcaa gaagcagtgc tcttgcaata
     1681 gcgactcttt gcctttgtcc accagaaagc gttactcccc tttcaccaac cactgtatca
     1741 aagctttgtg ggaacttctc gatctcgtcc tttatctgga ccatctctgc cactttttcg
     1801 atttcttctt tggtggcatc aggtttagca aaagctatgt tttctccaat tgaagtggag
     1861 aaaaggaaat tgtcttgcgg aacatagcca aaagatttcc tgagcatctt gaggtcccac
     1921 ttttttatat caacaccatc aatgctcact tcgccagaat cagagtccca gactctaagc
     1981 aaaagttttc caaaagagct tttccccgaa ccaaccgggc caacaacacc cacaacctgc
     2041 ccctttggaa tagtaatgga gattccgtct atggagttga tgtttgtacc ataagacaat
     2101 ttgacatctt tcagctctat gtttccctca atggtttctg gtttgtatgg gttttgtgga
     2161 gatttgatat caggaacaac ttctgtgatc tcaacaatcc tcttgagcga agcatttgac
     2221 ctttggtaaa gatttgttac ccatccaaga ttgatgattg gccagatcat gagactgaca
     2281 taggcgataa aggcagacag accaccaagg gtcaactggc ctttcatata taaaacacca
     2341 ccataccaaa ggattaccag tattggcatc cagcttgcca tgttcaggac aggccagaag
     2401 agggcatcca ttatattgag aggtttgatg gtctgaacgt atttgtccat cattcccttg
     2461 tacatcctct tctcatgatc tccctggttg tacgatctta cagttctggc accagagtag
     2521 ttttcctgca ctctgcttac aactttgtca gaatagtcct gaagaatctt gtaaagcttg
     2581 aatatctttt ttccaaaaag atattcgatg aagaagagta ccggcacaaa aagtaaaatt
     2641 ggtatggcaa gcttccatga tattgatatc atcaatatcg taccaagtac tgttctcaaa
     2701 acagcgtttg ggaaaagatt tccacccata ccaagaaaca tcgtgataga ctggacatca
     2761 gaagatgccc tgttcattat ttcacctgta gacatcccga caaaaaattt gtcaggcaat
     2821 ctcaagattt tatcatagat ggtgtttctc agatcataag ctgtgtgtct tgatacacca
     2881 acaagtgtgt acctgctggc tacaaacgta aaggacctca caacagcaat gccaacaacc
     2941 caaagcaaag ccgacaaaat tatatatttt gcatctggtt tcttaagata tgcagcatca
     3001 atgcttgttg aaataaacat tggaacaagc atgtctgagg tgttcaccaa aacgccgatc
     3061 atggccaaaa catatttcca tctgtatggc ttaagcaatc tagaataata ttggaatgta
     3121 gtcacggggc aaatattgcc acaaatactt tttggtatca agtgtataag ggtattgatt
     3181 gtaaccagct tattacgtta caatcaatac caaggaggtg ttaaatggca ggtttttggc
     3241 aaagaatcag cgatatattg caatcaaacg ttaatgattg gttgacaaag atggaagacc
     3301 cggagaaaat gcttcccctc aaaatcgacc agatgaagca ggacctggtg gaagccactg
     3361 atgcggccgc cgagtcgatg gctgtttcaa aacagaccga aatgagactc gctgatatca
     3421 agtttgatct caaaaagtac aatgatgcag cgcaacaggc actttccgca agcaaggaag
     3481 agcttgcaaa aaaatgtatg gccagagtat tgacgctcga gaaacagcaa aagagcgcag
     3541 aagagtcaca tgtcaggcaa gttgagctga acgcaaagct caaagatgtc atcgccgccc
     3601 tcaaggccaa aatcgaagag gctgaggtaa aaatggatga gctcatagcc aggaacaaag
     3661 cggcaaaagc gcaagagaag gtcgtcaaaa caatggggaa catcaatgtt gccgacattg
     3721 gcacaacatt caacgagctt gagcagaggg tcatcaagaa agaaaacaag gttgcggccc
     3781 tggcagaact cggttctgca gccgattcgc ttgatgacga gatcgcctcg ataaacgagg
     3841 aggatgaaat caacaagagg ctccagcaac tgaaaggcaa ataatgaatg tattgctgtt
     3901 tttactcggc gctgttgttg caggcattct acttggagta atactttact tcactaaaaa
     3961 gtctgttatg gacaggatac aaaaagaaaa acggtcagca atgggtgacc tggacaaaag
     4021 gtattttgcc atcagtgatg atctgaagct catacaggac atgggtgatt tgttatctgg
     4081 aaaatataaa aatgccacac cggaaaaagt tgccaggata aaagaacttg catcctcgtt
     4141 cgaatccggg tggattgcca gaagaaaaat ggtccaggac ccttccaacc agactgaagc
     4201 aacaac
//
LOCUS       DAOSRI010000154         4185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19141_length_4185_cov_3.181114, whole genome shotgun sequence.
ACCESSION   DAOSRI010000154 DAOSRI010000000
VERSION     DAOSRI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19141_length_4185_cov_3.181114"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PK717_05390"
     CDS             complement(<1..908)
                     /locus_tag="PK717_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47130.1"
                     /translation="MRKINLAIAILFLISAVFQFFDPMNSFSVKCDWLMYMNGPEHSG
                     NASSDCAPSSKDFKKTWEFKTGGGIGSSSVIKDGLMYFGNASNMFYCVDINTGSKKWD
                     FLIGESCKNEQKATIGGTISTPALFDGKVYFGTICGSVLCLDEKTGKKLWEYVNRDKV
                     ESFNSAIIIADEKIFCVSTAQVVYCLDAKTGKEIWKTVVLNNEISSPCFYDNEIYIAS
                     SYFLKCLNAKSGKEQWTYWHRGKTVGYSEPCIYGGKVFFGTIDSLLCSFSTSGGEKSW
                     EREMPKCVTGIASDKSCIYFGGLDKK"
     gene            complement(1231..>1928)
                     /locus_tag="PK717_05395"
     CDS             complement(1231..>1928)
                     /locus_tag="PK717_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HOR47131.1"
                     /translation="FYGRVEFKITKNAGKSVYGEKDKFTYTIFVHNVGSGDAVDVILT
                     DVFPRELEFVSSRPSATASNTSVRFSVGSLRVGGAFTATLTFKLSEKIEVPENGLTLT
                     NLATVQGKHHTSGQQDSKTDYASIFIPRQQSVEVLQFEVNWKGIDLKTGNARLGNEVE
                     LRVRAKGGSSPYRLTCSFDDNSADQSADLKNSDSEAVFKHAFSQAGDYNVIVTCTDTY
                     GTTKRVERIIHLK"
     assembly_gap    1929..1938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1939..>4185)
                     /locus_tag="PK717_05400"
     CDS             complement(<1939..>4185)
                     /locus_tag="PK717_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47132.1"
                     /translation="CWSGTGSGQSVARNIQVITDQSVCGPGTQPAGNRYYYYDQGSDR
                     GTINMSDRQIYVVTLRDIDMSMSGNAMPPQTSIKKFRMLIEDWGLGSGNEQIPLQMYW
                     NINHPNPPNIFWSPGMSAPRLESIDETNTVQRTMDFAPGTMGSGATSANTPSQVPGTF
                     DVRLVMGQIESGPDIGRWNVIPYYRLPSGGWTPFPGGAWMTPTAFDLTQARLTLQINS
                     DSDGWTIFTPPTVNAIDPFESYVDDDWVGLPYMAEVNFPCDLGNIHYIGVDAFPSINQ
                     AYSAAIPGANINVAPGDYQETVAINQAKDDITIKGVCWTDPGLLPISVTRPHISGPNG
                     SLIFANSAQINNNRFEDLIITNGIQFVNYAPINGVSFDFVYFMGTFPGQVPIFAMNQP
                     PTGIVSNFYMENCAIDGENNINRAGIGGNRFAGDFTILSTEFTRILNWGAVMDIDIAS
                     DYSPYGGNGFPLDVVTFSDNYIHHCNGSVSLRGHYIQKTTQVFASNNRWETIGGNQGL
                     QGEQWAALEVNHASYARIDNNFVNGVVLGMWGEGQAFQLWDIAILDCFRNTIINNYQG
                     IWIFGGEAQGSYGGPWPIPGGSIHHNIICGNQEYGLSVDPGCSGANPLLAENNWWGAN
                     DGPEKDGHGDEVIGQADFNPWLVLKTFALPARILIGGSESTITGDMRINSNDVDTSAQ
                     GFLPDNQILVNFKTNFGTLNGSGVNASAYVIGGVATVILRSSNVEQIATVCASAECKS
                     EYSEACVKV"
BASE COUNT         1088 a         1046 c          863 g         1178 t
ORIGIN      
        1 atcttcttgt ccaatccacc aaaataaata caagatttgt cagatgctat tccggttacg
       61 cacttcggca tttccctttc ccagctcttc tccccaccag atgtgctaaa cgagcacagc
      121 aggctatcta tggtgccaaa aaacactttg ccaccatata tgcagggctc gctgtatccg
      181 actgtttttc cacggtgcca gtaagtccat tgctccttgc cactcttggc attcaggcat
      241 tttaaaaaat atgagctggc aatatatatt tcattgtcat aaaaacacgg agaagaaatc
      301 tcgttgttca acacaaccgt cttccagatt tcttttccgg tttttgcatc aaggcaatag
      361 acaacctgtg cggttgagac acagaagatt ttttcatccg caatgatgat tgcgctattg
      421 aaagattcta ctttgtccct gttcacatat tcccacagct ttttgcctgt cttctcatcc
      481 agacacaaaa cgcttccaca gattgtccca aaatagactt tcccgtcaaa aagcgcaggt
      541 gtcgaaatag ttccgccaat tgttgccttc tgctcattct tgcagctttc gccaataagg
      601 aagtcccatt tttttgaccc ggtgttgatg tcgacacaat aaaacatgtt acttgcgttg
      661 ccaaaataca tgagcccgtc ttttatcaca gaagatgaac caattccccc accagttttg
      721 aattcccagg tttttttaaa atcctttgag gacggtgcgc aatcactgct tgcgtttccg
      781 gaatgctccg gcccattcat atacatgagc cagtcgcatt tcacggaaaa ggaattcatt
      841 gggtcaaaaa attggaagac tgcagaaatc aagaataata ttgcgattgc aaggttgatc
      901 tttctcatgg tacctccttt accagaccgc atattatact ttatttgtgt caataattct
      961 tcacaaattg tttacaggtt tcaagatcaa tgtcaggttt attttgcaac ttattttatt
     1021 aaaatgacgc tacatcaata aaattgggtc tgaaaatgct caaattcttt gatacagacc
     1081 ccgcgaaatt ctgagcttca aaagaggagc ctgaagactt ggaaaacaat gcatcattta
     1141 gatatgatga caactatatt atttatgcca aaaatagggc cagcataaaa tctactgctg
     1201 gcccagtgaa cagtgaacaa ctttactcaa ttattttaaa tgtattatcc tctctactct
     1261 cttcgttgtg ccataggtat cagtgcaagt gacaataaca ttatagtctc ctgcctggct
     1321 aaaggcatgc ttgaatacag cttcgctgtc ggaattcttt agatcggcac tctggtcagc
     1381 actgttgtcg tcaaacgagc atgtcaacct gtagggcgag gagccacctt ttgccctgac
     1441 ccgcaattct acttcgttgc caagccttgc attgcctgtc ttcaggtcaa tgcccttcca
     1501 gttgacctca aactggagaa cttccacaga ttgttgtctt ggaataaata tagaggcata
     1561 gtctgttttt gagtcctgtt gtccggatgt gtggtgcttg ccctgaacag ttgcaagatt
     1621 cgtcagcgtt aggccatttt ccgggacttc tattttctcg gagagtttaa atgtgagcgt
     1681 ggccgtaaat gcccctccaa ccctcaaaga gccgacggag aatcttacag aagtattgga
     1741 agctgttgca gagggtctgg aggacacaaa ttcaagctcc ctcgggaaca catcagtaag
     1801 gatgacgtcc acagcatctc cgctgccaac attgtggaca aagatggtat atgtgaactt
     1861 gtctttctca ccatagacag attttccggc gttctttgtt atcttgaatt ccaccctgcc
     1921 atagaactnn nnnnnnnnca ccttaacgca agcctcggaa tactcagact tgcactcggc
     1981 actggcgcaa acagttgcta tctgctcaac atttgaagac ctgagaatta cagttgcaac
     2041 gccacctatg acatacgccg aggcattaac accacttcca ttcagagtgc caaaattcgt
     2101 cttgaaattc accagtatct ggttgtctgg caagaatccc tgggccgagg tgtccacatc
     2161 gttgctgttt attctcatgt cgccggtaat ggttgattca gatccaccaa tcaaaattct
     2221 ggctggcaag gcaaacgttt tgagcacaag ccatggattg aaatcggcct gcccgatcac
     2281 ctcatcgcca tgaccgtctt tttctgggcc atcatttgca ccccaccagt tgttttctgc
     2341 caacaatggg tttgcccccg agcaacccgg atcaacagac aggccatact cctgattgcc
     2401 acagatgata ttgtgatgga tcgaccctcc agggattggc cacggtccgc catagcttcc
     2461 ctgggcttcg cctccaaata tccagatccc ctgatagttg tttatgatcg tattcctaaa
     2521 acaatccaga atggcaatgt cccagagctg gaatgcctgg ccctctcccc acatgcccaa
     2581 gacaacacca ttcacaaaat tgttgtcaat cctggcatag cttgcatggt tgacctccag
     2641 agctgcccac tgttcaccct gcaggccctg gttgccgcca atggtttccc atcgattatt
     2701 tgatgcaaaa acctgcgtcg ttttttgaat ataatggccc cttaaagaga cggacccatt
     2761 acaatgatga atgtagttgt cagagaaggt aacaacatcc agcgggaacc cgttgccacc
     2821 atatggcgag taatcggatg caatgtcaat gtccatcacg gctccccaat tcagaatcct
     2881 ggtaaattcc gttgacagga ttgtaaaatc acccgcaaag cggttgccac ctataccagc
     2941 cctgttgata ttgttctcac catcaatggc acagttttcc atgtaaaaat tactcacgat
     3001 tcctgtcggg ggctggttca tggcaaatat tggaacttga ccggggaacg ttcccataaa
     3061 atacacaaaa tcaaaggaca caccatttat tggggcataa tttacgaatt gaatgccatt
     3121 tgtaataatc aaatcttcaa acctgttgtt gtttatttgt gcgctgttgg caaaaatcaa
     3181 acttccatta gggccggaga tgtgtggtct cgtaacactt attgggagaa gtcccgggtc
     3241 tgtccagcat acacctttga tggttatgtc atcttttgcc tgattgatcg caactgtctc
     3301 ctgatagtca ccaggtgcaa catttatgtt tgcgccagga atggcggccg agtatgcctg
     3361 gtttatggac gggaaggcat ccacaccaat gtaatgtatg ttgcctagat cacacgggaa
     3421 attgacctct gccatgtacg gcaaaccaac ccagtcatca tcaacatagg attcaaaggg
     3481 atctattgca ttgactgtag gcggcgtaaa aattgtccac ccatccgaat cactgtttat
     3541 ctgcaaagtc aaacgtgcct gtgtaaggtc aaatgccgtt ggtgtcatcc acgcgccacc
     3601 ggggaagggt gtccagcccc ctgaaggcaa tctataataa gggattacat tccacctgcc
     3661 aatatctggc ccggattcga tttggcccat caccaacctg acatcaaaag tccccgggac
     3721 ctgtgaaggt gtgttggcag atgtcgcgcc agatcccatt gtgcctggtg caaaatccat
     3781 tgtcctttga acagtgtttg tttcatcgat actttcaagc ctgggcgcac tcatcccagg
     3841 actccaaaaa atatttggcg ggttgggatg gtttatgttc cagtacattt ggagaggaat
     3901 ctgctcgttt ccagaaccca gtccccagtc ttcaatcagc atcctgaatt tctttattga
     3961 tgtttgtggg ggcattgcat tgccagacat ggacatgtct atatccctca gtgttaccac
     4021 ataaatctgg cgatctgaca tgtttattgt gcccctgtca cttccctggt cataataata
     4081 gtagcggttt cccgcaggct gcgtgccagg gccgcacaca gactggtctg ttatgacctg
     4141 gatattcctt gcaacactct ggcccgaacc tgttcctgac caaca
//
LOCUS       DAOSRI010000155         4181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19159_length_4181_cov_3.938682, whole genome shotgun sequence.
ACCESSION   DAOSRI010000155 DAOSRI010000000
VERSION     DAOSRI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19159_length_4181_cov_3.938682"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK717_05405"
     CDS             complement(<1..466)
                     /locus_tag="PK717_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOR47133.1"
                     /translation="MNLSSLSTGKKVRMIRMAFEHGPGNGKLMILPIDQGLEHGPKDF
                     FANPESLDPKFEFELALRGAYSAIALHIGLADKYMCDYAGKVPLVLKLNGKTNIPPDD
                     EAFSSLTSTVEDAVRIGADAVGYTLFVGSPRQDDDIAQLCHVRQECDRFGMPL"
     gene            complement(553..963)
                     /locus_tag="PK717_05410"
     CDS             complement(553..963)
                     /locus_tag="PK717_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47134.1"
                     /translation="MINYNLVGFAGSDSSWIGYGAMLMYIVIFVCFMLGNWLSSKLMK
                     PAYPGWGRILYGALLLLCTNIVFSLIFFQMPLLATIFTAVAWYFEVKFLCGYSWSKAI
                     VGWLLPIAVAVIVVLILWLIWPSWIVSVLGFKLI"
     gene            1053..1493
                     /locus_tag="PK717_05415"
     CDS             1053..1493
                     /locus_tag="PK717_05415"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOR47135.1"
                     /translation="MERKTFRYFVSVDWEKGKQGFFSGINNEKFAFSCPPEFGGPEGF
                     MSPEEIFVASIATCVMATFINVCSRNKIGIESYNSKAEGVMEHIEGNYRITKVFLRPQ
                     IIIKDPEQKEECERILEDAHQTCPVGKSVVSQVILEPELSVSEM"
     gene            complement(1482..2717)
                     /locus_tag="PK717_05420"
     CDS             complement(1482..2717)
                     /locus_tag="PK717_05420"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOR47136.1"
                     /translation="MIAFLKICGLLIAVIIASAKKVPISITLIVAAVAAGFLFMPLEK
                     IPLTFLKIIFSWDTWKYVLALWAVLLLEGSLNKMGMLKSLASSSVKMFTDRRYACAVL
                     PSVIGFMPSVGGAYFSAPLVHEIAKDMDISKEKKAFINYWFRHIWESSIPLYPGIFMA
                     SAITGFSIPKVMAFQFPVTILGAFTGFAVAYKGVKRTQGEKGQISKRVLEFLKDAWPI
                     LLAIIFVLALPTEIVVSILLSIAILWIVIIVKVEKGQVEGKFAGRVFVDSVKESFRPN
                     YFIILLGSILLNQVLQNSTAFGELSVWFNSTGIPPVFVAIVLPMILGMLIGATPGFVG
                     VSFPIILAIAGNSSYGIFALAFTSGVIGVLFSPMHLCIVLTCDYFRTTLFKVFKLMLF
                     PAIAQMALGILTWWVSLHL"
     gene            complement(2714..3871)
                     /locus_tag="PK717_05425"
     CDS             complement(2714..3871)
                     /locus_tag="PK717_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR47137.1"
                     /translation="MKKRYGTKAIHSGEHPEWFNQAVTGDISPSTTYVIPNAKSLEGW
                     DGYIYSRISNPTRKSLEEKIASLDDCESAVALSSGMAAVSVGLMSCLSAGDIIVAQNS
                     LYGGTYDLVVNVLPKFGVKTLFVPKEKLYNLSWAPKEAKVAYIETPSNPTLGITDIRA
                     TCTAAHGRDMLVVADNSFATSYLQKPASLGADLVVYSATKFMAGHSDCLAGAITGRKD
                     LVEKAYRLMKQFGSPLDPFSCFLLARGLKTLELRMNKAQSTAMEIARFLANHPKVARV
                     FYPGLWNSPERELALTQMKGWGGMVTFEMKGGFEGASALFDNLQLIFRAGSLGGIESC
                     ISIPVLMSHHHLDEAALKEADITSGMLRLSVGIEDNSDLIEDLKQALDKVK"
BASE COUNT         1230 a          981 c          937 g         1033 t
ORIGIN      
        1 tcagcggcat cccaaacctg tcgcactctt gcctcacatg gcagagttgc gctatgtcat
       61 cgtcctgcct tggtgaacct acaaagagtg tgtatcctac agcatcagcg ccgattctta
      121 ctgcatcctc gacagttgat gtaagtgaag agaaggcctc atcgtctggt gggatgtttg
      181 ttttcccgtt cagtttaagt acaagcggga ctttccctgc ataatcgcac atatacttat
      241 ctgcaaggcc aatgtgcaag gctattgcag aataagcacc tctcaaggca agctcgaatt
      301 caaactttgg atcaaggctt tccggattgg caaaaaaatc ttttgggcca tgttccaggc
      361 cctggtcgat tgggaggatc atcaatttac cgtttccagg cccgtgctca aacgccatcc
      421 ttatcattct gacttttttg cctgttgaaa gactggatag attcatatta cacacctctt
      481 cttcaactgg gtcttggtca caagagccct tcgttgttgt ataaaatcct tgaagctaaa
      541 cctggcagaa atttaaatca gtttgaagcc aaggacgctg acgatccatg atggccagat
      601 gagccagaga atgagaacta cgatgacagc aactgcgatc ggtaagagcc atcctacaat
      661 tgccttgctc cagctgtagc cgcaaaggaa ctttacctca aaataccatg ccacagcagt
      721 aaaaatggtg gccagaagag gcatctggaa gaatatgaga ctaaaaacaa tgtttgtgca
      781 gagaagcagg agagcgccat aaagtattct tccccaaccc ggatacgcgg gcttcataag
      841 tttggaagat aaccaattgc ccagcatgaa gcaaacaaaa ataacaatgt acatcaacat
      901 ggccccatag ccaatccagg aactatcgga gccagcaaaa ccaacgagat tgtaattaat
      961 catttgcgca ccacctttca gcaaccattg taattccggc aaaaatccag tcaagccttg
     1021 atttgccaac tgcaagacct tatcaattgg gtatggaaag aaaaactttc agatattttg
     1081 tcagtgtcga ctgggaaaag ggcaagcaag gatttttctc aggcataaac aacgaaaaat
     1141 tcgccttttc ctgtccgccg gaatttggag gaccagaagg tttcatgtcc ccagaggaga
     1201 tatttgttgc ctcaattgca acatgcgtca tggcaacatt cattaatgtc tgctcaagaa
     1261 ataaaatagg aattgaatcg tacaattcaa aggccgaggg ggtaatggaa cacatcgaag
     1321 gcaactacag gataacaaag gttttcctgc ggccgcaaat aataatcaaa gacccggagc
     1381 aaaaggaaga atgcgagagg atacttgaag atgctcatca aacctgcccg gttggcaaat
     1441 cggttgtttc ccaggtcata ctggaaccag agctgagtgt gtcagagatg taacgacacc
     1501 caccaggtaa gtattccaag cgccatctgt gcaatcgcag gaaacagcat caacttgaat
     1561 accttgaaga gggtcgtcct gaaatagtcg catgtgagca ctatgcacag atgcattggc
     1621 gagaaaagca caccaatcac gccagatgta aaggcgagcg caaagattcc gtatgacgaa
     1681 tttccggcaa ttgccaatat gatcgggaaa gaaacgccta caaaaccagg agttgcacca
     1741 atcaacatgc cgaggatcat gggcaaaacg atggcaacaa aaaccggtgg gataccagtt
     1801 gaattgaacc agacagaaag ctccccaaaa gccgtgctgt tttgcaggac ctggttcaac
     1861 agtatggagc caagcaggat tatgaagtag tttggcctga aagattcctt gactgaatcc
     1921 acaaaaaccc tgccagcgaa cttgccctcc acctggcctt tttcaacctt tacgattatt
     1981 acaatccaca gtattgcaat actcagaaga attgaaacga cgatttcggt aggaagtgcc
     2041 agcacaaata tgattgcaag caagattggc caggcgtcct tgagaaactc caggaccctt
     2101 ttggaaatct gccctttctc gccttgggta cgcttcacac ctttataggc aacagcaaaa
     2161 ccggtaaaag ccccaagaat tgtgaccggg aactggaaag ccatgacctt tgggatcgag
     2221 aaacctgtga ttgcggaagc catgaaaatg cccggataga gtggaatgga gctctcccaa
     2281 atatgcctga accaataatt gatgaaggcc tttttttctt tcgagatgtc catgtccttg
     2341 gcaatctcat gaacaagcgg agctgagaaa tatgcgccac caacagaggg catgaaacca
     2401 attacagaag ggaggaccgc acaggcatac cttctgtcgg taaacatctt cacggatgaa
     2461 gatgcaagcg acttgagcat acccatcttg tttagcgatc cttcaagcaa aaggaccgcc
     2521 cagagtgcca gaacgtactt ccatgtgtcc caagaaaaaa ttatcttaag aaatgtcaaa
     2581 gggatctttt caagcggcat gaaaagaaac cctgccgcaa cagctgccac aattagcgtt
     2641 atcgaaatgg gtaccttttt ggcagatgcg attatgactg ctatcagcag cccgcaaatt
     2701 ttgaggaatg cgatcatttt actttgtcca gtgcctgttt gagatcttct atgagatcag
     2761 aattgtcttc aattccaacg gaaagcctga gcattccgct ggttatgtct gcttctttga
     2821 gggccgcctc atcaaggtga tggtgcgaca tcaggaccgg gattgatata cagctttcaa
     2881 taccacccag acttcccgct ctgaaaatta actgaaggtt gtcaaaaaga gcgctcgccc
     2941 cttcaaaccc acccttcatc tcgaatgtta ccatgccgcc ccagcccttc atctgtgtca
     3001 gggcaagttc cctctcaggc gagttccaca gacccgggta gaacaccctt gccacttttg
     3061 ggtgattggc aagaaaacgt gcaatttcca ttgcggtgct ctgcgccttg ttcattctga
     3121 gctcgagggt ctttaaccct cttgccagaa ggaagcacga gaaaggatca agtggcgatc
     3181 caaactgttt catgagcctg tatgcttttt ctaccaggtc cttcctgcca gttatcgcac
     3241 cggcaaggca atcactgtgg cctgccatga attttgttgc tgaatagaca accagatcag
     3301 cacctagact tgccggtttc tggagatagg aagtggcaaa agaattgtct gcaacaacca
     3361 gcatgtctct gccatgcgcc gcagtgcatg ttgccctgat gtctgttatt ccaagtgttg
     3421 gattggatgg tgtttcgata taggcaacct ttgcttcttt tggtgcccat gatagattgt
     3481 aaagcttctc tttggggaca aaaagtgttt ttactccaaa ctttggcagt acattgacaa
     3541 ccaggtcgta ggtccctcca taaagtgagt tctgtgctac tattatgtcg cctgcagaaa
     3601 ggcaggacat aaggccaaca cttactgcag ccattccgga cgagagtgcg acggcacttt
     3661 cacagtcatc aagcgaggct atcttctctt caagcgattt tcttgttgga ttggagatcc
     3721 ttgaatatat gtaaccatcc cagccctcaa gcgacttcgc gtttggtatt acataagtcg
     3781 tgcttggcga gatatcacct gtaacagcct ggttgaacca ttctggatgt tcaccggagt
     3841 gtatcgcttt tgttccgtaa cgttttttca tgccaaatct ccttgcaagg aaaaaagtga
     3901 ggattgtaaa aaaacatcat ggcaagaatg ttcgtgccac taataaagat aaccaagagc
     3961 cactttttga ctttgcaaag aagattgtga acatttttgc attaagtgtc caccgttgtt
     4021 atacttctat cacaaagttg cagcaaaaaa tgacagggtg ccaaaaaaat aaatggcccc
     4081 aaaacaacaa aaccacactt gaaaccttga acacataggc taagattgtt gaccaatagg
     4141 aggtcttgat gaaggaaaat gaaaatttac cagagttgat g
//
LOCUS       DAOSRI010000156         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19322_length_4155_cov_4.641951, whole genome shotgun sequence.
ACCESSION   DAOSRI010000156 DAOSRI010000000
VERSION     DAOSRI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19322_length_4155_cov_4.641951"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK717_05430"
     CDS             <1..416
                     /locus_tag="PK717_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47138.1"
                     /translation="AGNQTESGVVVFRDCTVNTALGSNMAFVNADEEWLLNPTQLISN
                     RSMVPLRDYCQFFGAQLIWDAPTRKLVLTLGRRKTQFQIGSSKAIVDGKSATVDPVPQ
                     IIFGSTYVPARFFAQAIGGGVGWHKESKTLSVSVP"
     gene            complement(451..1848)
                     /locus_tag="PK717_05435"
     CDS             complement(451..1848)
                     /locus_tag="PK717_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47139.1"
                     /translation="MKNKKLFIVLVSTLALVGVAFFSYKLIFPWRPHTQKLKLAYDSR
                     ISGDLICVLEIYSEDLTTLRPILISDNAIKICEDSALQKVLKELPIPGRALQTYVYIR
                     NGQVHEFPPKEGTEYLSYDDYAHIFSICKTGNGTNSLVSYVVPSSHKDKNDLKAISGT
                     YKAFEVPTDATRIITVFNDIAGGIPLVACQHDDLKHISFYDFKGKFVKSLDLQKQILF
                     RKPVSGHYIQSDCLGYDGDNLLVFDYYENDPVFKPTKTIPLGQIGTISKATFDIAFQN
                     FSVNHNIALFDGKSTFVFNSDFIGDRRYNEDNTKFYKFDGIEYSKSVNSYYFPTKDGL
                     YYLPPLGYKRYGINYNSLKKLSDEYLEPLAGETFFRIALDNIRAYFGKWEKGQYYLKV
                     AICFMDLHFRNYIVDFEPIPEKITSVIVSFDDEKNKDYIYAITENKVYDLMDTIIIAN
                     TFELLKYFDEHGVTK"
     gene            2052..3119
                     /locus_tag="PK717_05440"
     CDS             2052..3119
                     /locus_tag="PK717_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47140.1"
                     /translation="MRKLLAFALALVFLFASTGALGAEVPVGMSSFMALAIRSSAGKT
                     VVGLNGKVPLFWFDGKTFFICEIETAKTKDGAEYKKPKTDKEGKPVICAPSSKDFTKK
                     ALQSLTSGIYCILEPQEAIAVCVRLENTCLYGVFSKQGAIRFSQIGADRATKIGLTKP
                     NMVYILDQDYNRFVLALGNPQKVVSQEAPNPDTADPERCLGIKDVLPYECLSFEQLGR
                     GHLQIWTGKEEIVSCGIRSSCPQVTATHQGSVAASLELLCAQLGCSLEKGTEQNEFVV
                     HRFVSLLWEKPATIELRLKIGQFVGSADGKRLDFPFPPYLDQKTKKPMVPLRAFCNAL
                     GARVHWRALDSSALVERFVRK"
     gene            complement(3123..3677)
                     /locus_tag="PK717_05445"
     CDS             complement(3123..3677)
                     /locus_tag="PK717_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOR47141.1"
                     /translation="MKNKCLGLVLSARKGNCWDFARHVLPESDILVLGELYLEPCSKC
                     NYECMKDMACPKNDDIGKIYKKWMESDTVVLFSPVYDGRPPSLYYIFEERTPSLWKRR
                     CSGFASFGNRNAAIVVVGNENVERAEEILSRSLENLGLKIITSIGIHPNDYPAGGGIK
                     GGLIANREIMAKLDIFKNSILQSR"
     gene            complement(3765..4127)
                     /locus_tag="PK717_05450"
     CDS             complement(3765..4127)
                     /locus_tag="PK717_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOR47142.1"
                     /translation="MKINKVICVFVVALVLLSGGCVKSGAGKLEINAGGEYLYECSDG
                     STVKANFYSLSDDSLYFVKVNMENKEYTLPQLVSASGARYSDEREVEFWIKGDEVTIS
                     RMNDDGEWSVVKTGKIKK"
BASE COUNT         1126 a          950 c          861 g         1218 t
ORIGIN      
        1 aagctggaaa ccagacagaa tcaggagttg tggtcttcag ggattgcacg gtcaacactg
       61 cccttggctc caacatggcc tttgtgaatg cagatgagga atggcttctc aacccaacgc
      121 aactcatatc aaacaggtcc atggtgcctt tgagggacta ctgccagttt tttggcgccc
      181 aactcatctg ggatgcccca accagaaaat tagtattgac ccttggcaga agaaaaaccc
      241 aattccagat tgggtcatcc aaagcaatag tcgatgggaa atctgcaaca gttgatcctg
      301 tgccacagat catctttggc tccacctatg tgccggcaag attttttgcc caggcaattg
      361 gcggtggtgt cgggtggcac aaggaatcaa agacgctttc ggtttccgtg ccgtagttat
      421 tgcaagcagt tttgtgttga aaatgaaata ttattttgtc acaccatgct catcaaagta
      481 tttgagcaac tcaaaggtat tggcaatgat tatcgtgtcc atcagatcat agactttgtt
      541 ttcggtaatg gcatagatgt agtccttgtt cttttcatcg tcaaagctca caattacact
      601 ggtgattttc tcagggatag gctcaaagtc gacaatgtaa ttgcgaaaat gtaggtccat
      661 aaaacagatg gccactttca agtagtactg gcctttctcc cattttccaa agtaggccct
      721 gatattgtcc aaagctatcc tgaaaaatgt ttccccagcc aatggttcaa gatattcatc
      781 ggatagtttc ttcaacgaat tataattaat gccatatctt ttatatccca agggaggaag
      841 atagtacaat ccgtcttttg ttggaaaata atagctattt actgatttgc tgtattcaat
      901 accatcaaat ttgtagaatt ttgtattatc ttcattatat cgtctgtcac cgataaaatc
      961 actgttgaaa acaaatgttg attttccatc aaacagggca atattgtgat taactgaaaa
     1021 attttgaaat gcaatatcaa atgtagcttt tgagatagtt ccaatttgac caagtgggat
     1081 tgtttttgtg ggcttgaata cagggtcatt ttcatagtag tcaaaaacaa gcagattgtc
     1141 accgtcatag ccaaggcaat cggattgtat ataatgacca cttactggtt ttctaaacaa
     1201 tatttgtttt tgcagatcaa gtgatttgac aaatttgcct ttgaagtcat aaaaagagat
     1261 gtgtttcaag tcatcgtgct ggcaggcaac aagaggtatt cctcctgcaa tatcattgaa
     1321 gacagtaatt atccttgtgg catctgttgg gacttcaaat gccttgtatg ttccgctgat
     1381 tgcctttaaa tcattcttgt ccttgtgtga agagggaacc acataactga caaggctatt
     1441 tgtcccattt cccgttttgc atatggagaa aatgtgtgca tagtcatcat aactcagata
     1501 ttcagttcct tcctttgggg ggaattcatg tacttgtcca ttcctgatgt aaacataggt
     1561 ctgcaaagct cttccaggta tgggaagttc tttcagcacc ttctgaaggg cactgtcttc
     1621 acatatcttg attgcgttat ccgaaatgag gattggtctt aatgttgtta aatcctcaga
     1681 ataaatctca agaacacata tcagatctcc actgatcctg gagtcatatg caagcttgag
     1741 tttttgtgtg tgcggtctcc aggggaaaat gagtttatac gaaaagaaag caactcctac
     1801 cagtgccagt gtagacacta ggacaataaa tagttttttg tttttcattt tcctccgctt
     1861 cagtctgctt tgtgctcaca cataaatatt attcaaatta tactttttca gcaaatcttc
     1921 caagtaattt tttgaatttg tgtccgcatc aaaattgcaa ttcattgcta atttattgct
     1981 ggaaagcgtt tctgcggcta caatctcctc caccattcaa ttccggatgc aaaagtgtta
     2041 tcatttgcag tatgagaaaa ctgctggcct ttgcgcttgc gctggtcttt ctgtttgcta
     2101 gcaccggggc ccttggcgca gaggtgcccg ttggaatgtc atcttttatg gcactggcca
     2161 tccgctcctc agccggcaaa acggttgtcg gcctgaacgg gaaggtgccg cttttttggt
     2221 ttgatggcaa aaccttcttc atctgcgaaa tagaaacggc aaaaaccaaa gacggggcag
     2281 aatacaaaaa accaaaaacc gacaaggaag gcaagcctgt catctgcgct ccatcttcaa
     2341 aagatttcac aaaaaaggcc ctccagtcgc tgacaagcgg aatatactgc atccttgagc
     2401 cacaggaggc aatagctgtt tgcgttcgcc ttgaaaatac ctgtctttat ggtgtttttt
     2461 caaagcaggg ggcaataagg ttcagccaga ttggcgcaga ccgcgccaca aaaatcggcc
     2521 tcacaaaacc aaatatggtc tatattttgg accaggacta caacaggttt gtcctcgcgc
     2581 ttggaaatcc ccaaaaggtg gtctcccagg aagcaccaaa tccggacacc gcagacccgg
     2641 agagatgtct gggaataaaa gacgtcctgc cgtacgaatg tctctccttc gagcagctgg
     2701 gcaggggcca cctgcaaatc tggactggca aggaagaaat agtctcttgc gggatcaggt
     2761 cgtcatgccc acaggtcacg gcgactcacc aggggagcgt ggccgcaagc ctggaactgc
     2821 tgtgcgcaca gcttggctgc tcgcttgaaa aaggcaccga gcaaaacgaa ttcgtagtcc
     2881 acaggtttgt cagcctgctc tgggaaaaac cggccacaat agagctcaga ctcaaaattg
     2941 gccagtttgt tggctctgcc gatggcaaaa ggctggattt tccattcccg ccctacctcg
     3001 accagaaaac aaaaaagccg atggtgccgc tgagggcgtt ttgcaatgcg cttggcgcca
     3061 gggtccactg gagggcgctg gacagctccg cgctcgtgga gaggtttgta aggaagtagc
     3121 aattatctgg actgcaaaat agagttttta aaaatatcca gttttgccat tatttccctg
     3181 tttgctatca agccaccttt tatcccacca ccagcaggat aatcatttgg atgtatcccg
     3241 atggaggtaa ttatcttcaa gccaagattt tcaagcgatc tggacaaaat ttcttctgcc
     3301 cgttcaacgt tttcattgcc gacaactaca atggcggcat tgcggttgcc aaaagaggca
     3361 aatccactgc atcttctttt ccacagagac ggggttcttt cttcaaaaat gtaatacaat
     3421 gatggcggcc tgccatcata aactggcgaa aatagaacaa cagtgtcgct ctccatccat
     3481 tttttataga tttttccaat atcatcattt ttagggcagg ccatgtcctt catgcattcg
     3541 taattgcatt ttgagcatgg ctccaggtac aactcaccca ggacaaggat gtcactttct
     3601 gggaggacat gtctggcaaa gtcccagcaa ttcccttttc tggcgctcaa aaccaacccc
     3661 aaacatttat ttttcatgtc tttttgaaat tataacctaa ttcaagccct tacagcaaat
     3721 taacgttgta aatatcgtaa tcgttgttaa taggttagat cgttttattt tttgatcttc
     3781 cccgtcttta ccacgctcca ttccccatca tcattcatcc tgcttattgt aacttcatcc
     3841 ccttttatcc agaactcaac ttctcgctcg tcgctgtacc ttgctccgga ggcagacacc
     3901 agctgtggca gtgtgtactc tttgttttcc atgttcactt ttacaaagta caggctgtca
     3961 tcggacaaac tgtaaaaatt agcttttact gttgaaccat cactgcattc gtataaatat
     4021 tctcccccgg cattgatttc cagcttgcct gctccgctct tgacgcaccc gccactcaat
     4081 aaaacaagtg ccacaacaaa aacgcatatg actttattta tcttcattca ttttcacctc
     4141 gctcttttat gttct
//
LOCUS       DAOSRI010000157         4120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19520_length_4120_cov_4.223862, whole genome shotgun sequence.
ACCESSION   DAOSRI010000157 DAOSRI010000000
VERSION     DAOSRI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19520_length_4120_cov_4.223862"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1229)
                     /locus_tag="PK717_05455"
     CDS             complement(84..1229)
                     /locus_tag="PK717_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR47143.1"
                     /translation="MDYFLTDEQKMIKNLAKKIAEEKIRPVRAELDEKGIFPMEIIKE
                     FAKADLMRTYIPKEYDGLGLGVLELCLIIEELARVDGGVAVSFAVNALGSFPIVIMGN
                     EQQKKKYLTKAAAGEWLTAFALTEANAGSDASNIATTAVKDGDHWVLNGTKQFITNGR
                     VADFTTVIAQADKSKGVRGLMAFIVEKGTPGFTYGKDEHKMGIRSSVTTELVFQDCRV
                     PAENVIGAEGQGFLVAMKTLDKSRPGIGAQGLGIAWGAYEAALLYAKQRVQFGQPIIN
                     FQGISFMLADMATQIEAGRNMLYNVARWIDSGATKYSKESAMVKLYCTDLAMKVTVDA
                     VQVFGGYGYMKEYPVEKMMRDAKITQIYEGTNQIQRSVIASNIVKDV"
     gene            1322..2677
                     /locus_tag="PK717_05460"
     CDS             1322..2677
                     /locus_tag="PK717_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47144.1"
                     /translation="MKRTFVLIMLVFFFCAGCGCNETTRIVKVDDSGNNKSVADNQNE
                     TTNNAPPVKVDPFAEKPFPKAEPVKEWFMFKGTPDNLGLVDADIKLPLGKKYDEKNKK
                     AVEYRWVSFSAPNMNRLLGNPAVVRNRIYFGCEMGFISCFDFITGEPKWRNGDQIKPI
                     ITPIAANDYVVVFGTLDGEVYCYDGLSDSQLWVFKTSPNISAPPNITDENVKNGRVHG
                     GPKIVDDKVYFGAFDGKLYCLNIKDGKKVWEFATKGKIYSSPAISDGRIYFANFEGKL
                     FCIDQKTGKKIWDADLGKPTIASPVVFGPRVWIGGKNSKMFCFQTKDGKKLWEYQGPI
                     SDYGIESTPALDEENLYFGETKGGLICLNWKTGKEVYKVSISDKPLNSSPVIVRNMVM
                     VGGHDKNFYVIDKKNGKVLDTFTTRGSILASPIIIGNEVLVTSYDSSIYILRAKGTNP
                     P"
     gene            complement(2680..3873)
                     /locus_tag="PK717_05465"
     CDS             complement(2680..3873)
                     /locus_tag="PK717_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOR47145.1"
                     /translation="MDLSERLQRIVGAPKSQAKKSSIPLEELISGEWSQKGQSKVFVA
                     FSSRTWTTKQRTYSRLKNFVGLGADISPERVLFLDTETTGLAGGTGTYAFMVGLGFLD
                     SGKFQTVQLFMPKFSDEPHMLSILREYFDGKTHIVTFNGKTFDIPLLETRYLMMRERN
                     PFSEFDHLDLLHTSRIIWKKRLQSCSLQSLECNLFRKIREGDIPGELIPEVYFDYIRT
                     NNPTDMARVFEHNQQDVVSMMSLLEMIGSIFETPDSPFFEDPIEQLSVGQYLWKKGFS
                     CEGMEYMQRATDTPNKKLRDEAMFALGRLCKKQGEFEKAAKHFENVCHDPYQVIEALV
                     EQAKHFEHRTKDIGRALEATEKAMSVARQIKLLSENEMAAPTIDGLRHRHERLVRKKS
                     RLDDH"
     gene            complement(3879..>4120)
                     /locus_tag="PK717_05470"
     CDS             complement(3879..>4120)
                     /locus_tag="PK717_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47146.1"
                     /translation="YEASTKVISFVTEAGKKVELKIGSKTAVVDGAPVQLQAAPQIVK
                     GKTMIPLRFVSEALGANVNYSASNKQITLTKPGCK"
BASE COUNT         1032 a          905 c          932 g         1251 t
ORIGIN      
        1 ttggcattat accttccctg tcaaaaaaga aaaaccccgc aatgaaactg cggggttttg
       61 ggttcgttct ttattaatca ggctcacaca tctttgacaa tattggaggc gatgactgac
      121 ctctgtattt gattagtgcc ctcatatatc tgggttatct tggcatcgcg catcattttt
      181 tcaacgggat actctttcat gtagccatac ccaccaaata cctggactgc atccacagtc
      241 accttcatgg ccaggtctgt gcaatagagc tttaccattg ctgattcttt tgaatatttt
      301 gttgcccctg aatcaatcca ccttgccacg ttgtagagca tgtttcttcc agcttcaatc
      361 tgggttgcca tatcggcaag catgaaactg attccctgga agttgatgat cggctggcca
      421 aactgcaccc tctgctttgc gtagagaagt gctgcctcgt aggcacccca tgctattcca
      481 agcccttgtg ctccaatacc tgggcgtgac ttgtcaaggg tcttcattgc aacaaggaag
      541 ccttgccctt ctgcgccaat aacgttttcg gcagggaccc tacaatcctg gaatacgagt
      601 tcggttgtga ctgaggaacg aatgcccatc ttgtgctcgt ctttgccata agtgaagcct
      661 ggcgtgccct tctcgactat gaaggccatg aggcccctta cccccttgga tttgtcagct
      721 tgtgctatga ctgttgtaaa atctgccacc ctgccgttgg ttatgaactg cttggtacca
      781 ttgagcaccc aatggtcgcc atccttgact gctgttgttg caatattgga tgcatcagag
      841 ccggcatttg cctctgttaa tgcaaatgct gtcaaccatt cgccagcagc ggcctttgta
      901 aggtatttct ttttctgctg ctcgttgccc attatgacga tcgggaatga accaagggca
      961 tttactgcga aagaaaccgc cacgccaccg tcaactcttg caagctcctc gatgatgaga
     1021 cagagttcaa gcactccaag cccgagacca tcgtattctt ttggaatgta tgtcctcatc
     1081 aggtctgcct tggcgaattc cttgatgatt tccattggga atatgccctt ttcgtcgagt
     1141 tcagccctaa ctggcctgat tttttcctcg gcgatctttt tggcgaggtt ttttatcatt
     1201 ttctgttcat cggtgagaaa atagtccatc tgtagacctc cttactttta ttttgacaca
     1261 actgatagaa actggttaca atatgtggaa catttttgtt ttttggcaag taaggagatg
     1321 tatgaaaaga acttttgttt tgataatgct ggtttttttc ttttgtgcag ggtgtggttg
     1381 caatgaaacg accaggatcg ttaaggtaga tgattctgga aacaacaaat ctgttgcgga
     1441 taatcagaat gaaacaacca acaatgctcc tcccgtaaaa gtggatccat ttgcagaaaa
     1501 gccctttcct aaagcggaac ctgtaaagga atggtttatg tttaagggca ccccggacaa
     1561 tcttgggttg gtggatgccg acataaagtt gcctttgggg aagaaatatg atgaaaaaaa
     1621 caagaaggcc gtagaatatc gctgggtctc tttttcagca ccaaatatga acaggcttct
     1681 tggcaatcct gccgttgtaa ggaacaggat atattttggt tgtgagatgg gcttcatctc
     1741 atgctttgat tttattacag gcgaacccaa atggaggaat ggcgaccaga taaaaccaat
     1801 tataactcca attgcggcca atgattatgt tgtggttttt ggtacccttg atggtgaggt
     1861 ttactgttat gatggtcttt cagattcaca attatgggtg ttcaagacta gcccaaacat
     1921 ctctgcacca ccaaatatta ccgatgagaa tgtgaagaat ggaagggttc atggcgggcc
     1981 aaaaatagtt gatgacaaag tttattttgg tgcttttgat gggaaactct attgccttaa
     2041 tatcaaagat ggcaagaaag tttgggagtt tgctaccaaa gggaaaatat acagctcccc
     2101 tgccatctcg gatggtagga tatattttgc caattttgag ggaaagcttt tctgcattga
     2161 ccagaaaaca ggcaagaaga tatgggatgc tgacttgggc aagccaacga ttgcatctcc
     2221 ggttgtcttc gggcccaggg tctggattgg cggcaaaaat tcaaagatgt tctgcttcca
     2281 gacaaaagat gggaaaaagc tttgggaata ccaggggcca atttcagatt atggcattga
     2341 gtcaactcca gcgcttgacg aagaaaacct ctattttggc gaaacaaagg ggggccttat
     2401 ttgtttgaac tggaagaccg gcaaagaagt ctacaaagtc agcatctcgg ataaaccgct
     2461 caattcctcg ccagtaatcg tcagaaacat ggtaatggtt ggcggtcacg acaagaattt
     2521 ttatgtcata gacaagaaga atgggaaagt gctggacact tttacaacaa ggggcagcat
     2581 tcttgcctca cccatcatta ttggaaatga agttctggtg accagttacg attcgagtat
     2641 atatatcctc agggcaaagg gaacaaaccc tccctgatcc tagtgatcat ctaatctgga
     2701 ttttttcctt accagtctct catggcggtg cctcaaacca tcgattgttg gcgccgccat
     2761 ttcattttca gagagcaatt ttatctgcct tgcaacggac attgctttct cggttgcttc
     2821 aagcgccctg ccgatgtctt ttgttctgtg ttcgaagtgt tttgcctgct caaccagagc
     2881 ttcaatcacc tggtatgggt catgacaaac attttcaaaa tgttttgcgg ctttttcaaa
     2941 ctcgccctgt tttttgcaaa gccttccgag tgcaaacatc gcttcgtctc gcagtttctt
     3001 gtttggtgtg tcagtagccc tttgcatgta ttccattcct tcacaagaaa agcctttttt
     3061 ccataggtat tggccaactg aaagctgttc tattgggtct tcaaaaaatg ggctgtctgg
     3121 tgtttcaaag attgagccaa tcatttccag taaagacatc atgctcacga cgtcctgctg
     3181 gttgtgctcg aacactctgg ccatgtctgt cgggttgttt gttcttatgt aatcgaaata
     3241 aacctctggt atcaattccc ctggaatatc gccctctcta atttttctaa aaaggttgca
     3301 ctcaaggctc tgaagtgagc aggactggag cctttttttc catattattc ttgatgtgtg
     3361 cagaaggtcc aggtgatcga attctgaaaa agggttcctc tccctcatca tgaggtatct
     3421 tgtttcaagg agtggtatat cgaatgtttt tccgttgaaa gttacgatgt gggtttttcc
     3481 gtcgaaatat tccctcaata tagagagcat gtgtggctca tcggaaaatt ttggcatgaa
     3541 aagctggaca gtctggaatt ttccagagtc cagaaaaccg aggccgacca taaaagcata
     3601 ggttcctgtt ccaccagcaa gaccggtggt ttcggtgtct agaaacaaaa ctctctctgg
     3661 agatatgtca gccccaagac cgacgaaatt tttgagcctt gaataggtcc tttgttttgt
     3721 tgtccaggtt cttgaggaga atgccacaaa aactttgctc tggccttttt gagaccactc
     3781 accgctgata agctcctcca gaggaatgct ggattttttg gcctgtgatt tgggtgcacc
     3841 aacaatcctc tggagacgtt ctgagaggtc catttttatt atttacagcc tggttttgtg
     3901 agtgttatct gtttgttgga tgcgctataa tttacattcg caccaagggc ctcgcttaca
     3961 aaccgtaatg gaatcatcgt ttttcccttt acgatctgtg gtgctgcctg gagctgaaca
     4021 ggtgcgccat caacgacggc tgtttttgaa ccaatcttca attcgacttt ctttccagct
     4081 tcagtcacaa aggaaatgac tttggttgat gcttcatatt
//
LOCUS       DAOSRI010000158         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19534_length_4118_cov_4.096234, whole genome shotgun sequence.
ACCESSION   DAOSRI010000158 DAOSRI010000000
VERSION     DAOSRI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19534_length_4118_cov_4.096234"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK717_05475"
     CDS             <1..427
                     /locus_tag="PK717_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47147.1"
                     /translation="KTATPETPRVEERKEQIGPVSIVVPKGFIWQTVGNSSATIKGDF
                     EIKIELFDCQGLKEGDLDSLSPIIQEITQGLKQEKEVQVENIGGKECITVNSSGAKNV
                     FYAAIFPLESQVAAIYTSKMPLKKTADMAIQVVRSTTTF"
     gene            463..966
                     /locus_tag="PK717_05480"
     CDS             463..966
                     /locus_tag="PK717_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47148.1"
                     /translation="MKKMFLACLCLFVLASCGAPKVDNQIPEELKFKFDGDLFEVYYP
                     DGWETLSSVGGGVNIFKKDKSITIPFSIFPYEGSRENDISSIGELSQAFEKTIGKTGQ
                     GTIETREISGKKALWMEGEVGMGKMQAFMVPLDNAVVSCMMFPGKGASAEDARLAGLI
                     VKSFRFK"
     gene            974..1921
                     /locus_tag="PK717_05485"
     CDS             974..1921
                     /locus_tag="PK717_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47149.1"
                     /translation="MKTTRIFVALCAILTLLSCGTEEKPLPLTETYFGPFYRIDFPKN
                     AKIEPVGNLTSIVTDKFVVEVKLEPSEGLKPGDFGGVAFLKEQSQKMADELGAKASFR
                     EAKLGDAPCLVIDMTGENIAWKRICVPLKNNIAVVSLKEPFEGEKAAESPFFKSASDM
                     IISFRITDSSYLGSETPSQNGNGGEIKLETRFENENLSMMLPSGWEAGGDGVVLVCPK
                     DIAKFDQGFTVTVFGENRDPKSACQSMSSSLSGGTIQKTRIGGNDYWMFEYSVGQTKM
                     VSMFSGQKGKVFLLSSTSFNGKPSRIAEKIIGSMSFKHP"
     gene            2037..2630
                     /locus_tag="PK717_05490"
     CDS             2037..2630
                     /locus_tag="PK717_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47150.1"
                     /translation="MKKFFATFIILVLGVAVFASCGGGSNDEGTKPADEGQNIEQTEN
                     QTGSQNNEQTPAATFANYAETKLLKIGIAEGWKNNTPEAPKDQQNGDIDIISLMSDDG
                     KAITVTSVKTGEIPVKIEDFVEQMKKNLVESKATSIEEGKASYAGIDYTTLTFSVENN
                     KAISLFGIKGNQVINISLNGTDIDAEIEGMLNSVETK"
     gene            2743..3696
                     /locus_tag="PK717_05495"
     CDS             2743..3696
                     /locus_tag="PK717_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47151.1"
                     /translation="MKKLVAFLLLALFAISCGGQPPTKHDGGWFEIEYDQGMKTLAEG
                     DTFMVFDDEIFMKVQPNMDYSNTEKIEVARQSAKQLVESMSKDTSNTVTERESKCGSI
                     PCIWVEVENKNQGTGGLIMYVPIQNVVVWVGLAQPVNLKTGMDKARRIAESFRIKDEG
                     YFKNRVEDFAKNYNVNGSSKTIETETFSITPAKEWAGEKDLKNSNVRLRLLPDSTKAK
                     QTPYAPNIEVGIMDKGQMALEEFLESFKNAMVNKDKVAGPKPFKFAEMMYYRYDLPAK
                     KDGAPLMYIVGEKDGKIIKALIAGELSGDIETMLESIKIKK"
     gene            3709..>4118
                     /locus_tag="PK717_05500"
     CDS             3709..>4118
                     /locus_tag="PK717_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47152.1"
                     /translation="MKKLQNILLTVIIGLTLISCGKTTLDETYEGPYFSISHPGSWKV
                     EENPSLTTISGDGVGIMIESVPNEQIKANDVAEIKTYSDQLLKELGGNEDFDLSTEEK
                     EINGQKGVWLLAKSKKEEKGGFMFITAVDKAVLMV"
BASE COUNT         1361 a          869 c          975 g          913 t
ORIGIN      
        1 aaaaaccgcc acaccagaaa ctccccgggt agaagagcga aaagaacaga ttggtcctgt
       61 ttcaattgtc gtcccaaaag ggtttatctg gcaaactgtt ggcaacagca gcgccacaat
      121 aaagggcgac tttgaaatca agattgaact ctttgactgc caggggctga aggaaggcga
      181 ccttgactcg ctctccccaa taatacaaga aatcacacag ggactcaaac aggaaaaaga
      241 agtccaggtg gaaaacatcg gcggcaagga gtgcatcacg gtcaattcaa gcggggcaaa
      301 aaatgtcttc tatgcggcaa ttttccctct cgaatcgcaa gtggcagcaa tctacacaag
      361 caaaatgccg ctgaagaaaa cggcagacat ggccatccag gtcgtaagat caacaacaac
      421 tttttgaaac aaacggcacc atttttgtgt ataatgcatg acatgaaaaa aatgtttttg
      481 gcctgcctgt gcctctttgt tttggcctcc tgcggcgccc caaaagtaga caaccagata
      541 cctgaggagc taaaattcaa gtttgatggc gatcttttcg aggtctacta cccggatggg
      601 tgggaaacac tctcctcggt tggcggcggg gtcaacatct tcaaaaaaga caaatccatt
      661 acaattccat tctctatctt cccctatgaa ggttccagag aaaacgacat atcaagcata
      721 ggcgagctgt cgcaggcctt tgaaaaaacg ataggaaaaa cagggcaggg aacaatagaa
      781 acccgcgaaa tatccgggaa aaaggcactc tggatggagg gtgaagttgg catgggcaaa
      841 atgcaggcct ttatggttcc actcgacaat gccgtggtaa gttgcatgat gttcccagga
      901 aaaggggcca gtgccgagga cgccagactt gcagggctca tagtaaagag cttccgtttc
      961 aagtgaggcg gagatgaaaa caaccagaat ttttgtggca ctttgtgcaa tacttactct
     1021 tctttcctgt ggaactgaag aaaaaccatt gcctctcact gaaacatatt ttggtccctt
     1081 ctacaggatc gacttcccaa aaaacgcaaa aattgagcct gtcggaaacc tcacaagcat
     1141 agtcaccgac aaatttgttg ttgaggtaaa attagagcct tcagaaggcc tcaagccggg
     1201 ggattttggg ggagtggcct ttttgaaaga acagtcccaa aaaatggcag acgagcttgg
     1261 ggcaaaagcc agctttagag aggcaaaact tggtgacgca ccctgcctcg taatcgatat
     1321 gacaggcgag aatatcgcat ggaaaaggat ttgcgttcca ctaaaaaaca acatagccgt
     1381 ggtatcactc aaagagcctt tcgagggtga aaaagctgcc gaatcgccat ttttcaaatc
     1441 ggcctcggac atgatcattt ctttcagaat aacagacagc tcctatcttg gctctgaaac
     1501 accatctcaa aatggcaatg ggggagaaat aaaacttgaa acgcgctttg aaaatgaaaa
     1561 cctctccatg atgctcccgt caggctggga ggccggtgga gacggcgttg tgcttgtttg
     1621 tccaaaagac attgcaaaat tcgaccaggg ttttacggta acggtctttg gagaaaacag
     1681 ggaccctaaa agtgcctgcc agtcaatgtc atcatcactt tcaggcggta caatccagaa
     1741 aacaaggatc ggagggaacg actactggat gttcgagtac tcagtcgggc agaccaaaat
     1801 ggtttcgatg ttctcggggc aaaaaggaaa ggtttttctt ctgtcttcaa catcttttaa
     1861 tggaaaaccc tccaggatcg ccgaaaaaat aattggaagc atgtctttca agcatccatg
     1921 actcattact attttgttga gattttatta agcttttaac gtcttgaaag aacaaattcg
     1981 cttcatatga tgtatgtgct tttgaaaatc ccaaaaggac agagaggagt ctcaacttga
     2041 agaaattttt cgcaactttt atcatactgg tgcttggcgt tgccgttttc gcatcctgcg
     2101 gtggcggatc aaatgacgag ggcacaaagc cagccgacga aggccagaac atagaacaga
     2161 cagagaacca gaccggaagc cagaacaacg aacagacccc ggccgcaaca tttgccaact
     2221 atgccgagac caagctactc aaaattggca ttgccgaggg ttggaaaaac aatactcccg
     2281 aagcaccaaa ggatcagcag aatggtgata tagacataat atcgcttatg agtgatgatg
     2341 ggaaggcaat taccgtaaca agtgttaaaa ccggagaaat ccctgtcaag atagaagatt
     2401 ttgttgaaca aatgaaaaaa aatctggtcg aaagcaaggc aacttcaatt gaagaaggta
     2461 aagcttccta cgcaggcatt gactacacta cacttacttt cagcgtagaa aacaacaaag
     2521 caataagtct tttcggaatt aagggtaacc aggttatcaa tattagcctt aatggcaccg
     2581 atattgatgc cgaaatcgaa ggaatgctca attcagtcga aacaaagtaa gaaccatttt
     2641 aaagaaccaa caaaaaaacc ggctcaaaac agccggtttt tttgttttaa tgacctttga
     2701 caagccaatt tttgggacta ctttttatgc ggaggaattt gaatgaagaa acttgttgct
     2761 tttttgctgt tggccctctt tgccatttcc tgtggcggac aaccacccac aaaacatgat
     2821 ggcggatggt tcgagataga atatgaccag ggcatgaaaa ctcttgccga gggtgataca
     2881 ttcatggttt ttgatgatga aatatttatg aaagtccagc caaacatgga ctattcaaac
     2941 accgagaaga ttgaggtcgc aagacaatct gcaaaacaac tcgtggagag catgagcaaa
     3001 gatacatcaa acaccgtgac cgaaagggaa agcaaatgcg gctcgattcc atgtatctgg
     3061 gttgaagttg aaaacaaaaa ccagggcaca ggcggcttga taatgtatgt accaatacaa
     3121 aatgtcgttg tgtgggtggg cctggcacaa ccagttaatc ttaaaaccgg catggacaaa
     3181 gccagaagaa ttgccgaaag cttcaggatc aaggacgagg gttatttcaa aaacagggtg
     3241 gaagatttcg ccaagaatta caatgtcaat ggatcatcaa agacaatcga aactgagaca
     3301 ttctctataa caccagcaaa agagtgggca ggggaaaaag acctgaaaaa cagcaatgtc
     3361 aggttgcgcc tgcttcctga cagcacaaaa gcaaaacaga caccctacgc accaaacata
     3421 gaggttggga tcatggacaa agggcaaatg gcactggagg agttcctgga atcattcaaa
     3481 aatgcaatgg taaacaaaga caaggtcgct ggcccaaaac cgttcaagtt tgccgaaatg
     3541 atgtattaca ggtatgattt gccagcaaaa aaagacggtg ccccacttat gtatattgtt
     3601 ggcgaaaaag atggcaagat aataaaagcc ctgattgcag gggaattaag cggagacatc
     3661 gaaacgatgc ttgaaagcat caagataaaa aaataaggag agaaaaaaat gaaaaaactc
     3721 caaaacattc tgctgacagt catcataggg ttgaccctta tttcatgcgg aaaaaccaca
     3781 ctggacgaaa cttatgaagg gccctatttc agcataagcc acccgggttc ctggaaggtc
     3841 gaggaaaatc cgtctctgac aacaatcagt ggggacggcg ttggaataat gatcgagtcc
     3901 gttccaaacg agcaaataaa agcaaatgat gttgccgaga tcaagacata ttcagaccag
     3961 ctcctcaaag agcttggtgg aaacgaggac ttcgacctct caaccgagga gaaggaaata
     4021 aatggccaga agggtgtctg gctcttggca aagagcaaga aggaagaaaa gggcgggttt
     4081 atgttcatta cggccgtcga caaggccgtc ctcatggt
//
LOCUS       DAOSRI010000159         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_19838_length_4071_cov_3.222112, whole genome shotgun sequence.
ACCESSION   DAOSRI010000159 DAOSRI010000000
VERSION     DAOSRI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19838_length_4071_cov_3.222112"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK717_05505"
     CDS             <1..495
                     /locus_tag="PK717_05505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOR47153.1"
                     /translation="EEIIGKAREDAKSILRIAEEEAKGEREITEQDLKSRSKKIIVQA
                     SRQVMKQVFDNGMTEAYTKHIISELPKLTVCDIHSHKLNLCDALSQAGKVSRIVIESA
                     TEMPEVDKKAVSDFVLGFVKNGAKVEFIIKPELISGLRIVFGDTIFDSSISRMVETTI
                     EDLR"
     gene            500..2008
                     /locus_tag="PK717_05510"
     CDS             500..2008
                     /locus_tag="PK717_05510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOR47154.1"
                     /translation="MPEILDEIINTWQASFEAKPFIPVIENAGSVSEVGDGVALVRGL
                     SDAFISEVLSFEGGIEGFVLSLAPDYVGVVLLGEDKYVREGDRVSRTGRLLSIPYTQN
                     LIGRVIDPLGNPIDGKPAPKSIKFLPLETKAPSVIEREPVHQPMQTGIKTIDALFPIG
                     RGQRELIIGDRQTGKTAIAIDTILNQKDENVICIYVGIAQKSSTMAGIVKTLRDYGAL
                     DYTLIVTTLADQSPATRYIAPYVGCAIGEELMRSGKHVLIIYDDLSKHADAYRELALL
                     LRRPPGREAYPGDIFYLHSRLLERAAKLHKDLGGGSMTALPIIETQAGDVSQYIPTNL
                     ISITDGQIYLEKELFHEGIRPAVNVGLSVSRVGGNAQIPTMKKVGGSLKLDLSQYNDL
                     KAFAQFGAELDPVTQKKLQRGEKLVELLKQPQYSPMHVGQQVLILGAYSLGALDDIRK
                     DRVQEFERVLVEEAKRFRPDLLKELSMAPKLTDAHKEGISKLVQSVSSTFGS"
     gene            2011..2886
                     /gene="atpG"
                     /locus_tag="PK717_05515"
     CDS             2011..2886
                     /gene="atpG"
                     /locus_tag="PK717_05515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOR47155.1"
                     /translation="MATLRTLKRRIASVDSVAHITRSMEMLSVVKIYHLRKKVEESVP
                     YVEKLRELLVSIASHTSTDLTANPLFIARPIEKVLIIPLTSDRGFCGAFNSNVMSLTK
                     RYSEELGLKSTFATIGKKANAELTKQGHEISFQMPGILDKLSFTQAQILSKKIKEMYI
                     SDEVQAVSFVYMEFQSVLRQESVVREMLPLVPIMKGHSIPLETFIFKPNPTAILDTLM
                     DNYVDLFVYKVLLEAGASEQAMRRMSMHRATENADDLGQKLTIKYQRTRQAAITKELT
                     EITSGAEAIKERKSV"
     gene            2879..>4071
                     /gene="atpD"
                     /locus_tag="PK717_05520"
     CDS             2879..>4071
                     /gene="atpD"
                     /locus_tag="PK717_05520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOR47156.1"
                     /translation="MSKGKLIRIEGPVLDFSFPKGELPDLYNVVVVEDPALPEKLVCE
                     VHQVVNATDVRCIALGSTEGLRRGMICQDTGAPINVPVGEGTLGRVFNVFGNPIDKKG
                     DVPAVEKRPIFHNPPQFSKINPTESIFETGIKVIDLLAPFPKGGKIGLFGGAGVGKTV
                     LLQELIRNVAYEHQGVSVFAGVGERTREGNELILEMEASGVIKSTALVFGQMNEPPGV
                     RMRVPFTALTMCEYFRDDKGQDVLLFVDNIFRYVQAGSEVSALLGRIPSAVGYQSTLA
                     TEVGSLEERIISTKKGSITSVQAIFVPADDYTDPAPANIFTHLDATITLDRALVELGI
                     YPAANPLDSSSKMLDPRIVGKEHYEVAQEVKRILQHYENLKDIIAILGMSELSEEDRK
                     LVYRA"
BASE COUNT         1160 a         1030 c          965 g          916 t
ORIGIN      
        1 gaagagatca ttggtaaagc aagggaagac gccaagtcca tactcaggat tgcagaggaa
       61 gaagcaaaag gcgaacgtga aataaccgag caagatctaa agagccgctc gaaaaaaatt
      121 attgttcagg cttcaagaca ggtcatgaag caggtcttcg ataatggcat gaccgaagcc
      181 tacacaaaac acatcatttc tgagctgcca aaactcacag tctgcgacat ccattcccat
      241 aaactcaatc tttgcgatgc gctctcccag gcaggcaagg tatcaagaat cgtcatcgag
      301 tctgcaactg aaatgccaga agtggacaaa aaggcagttt cagatttcgt cctcggcttt
      361 gtgaaaaacg gcgccaaggt tgaatttatc ataaaaccag agctcatttc tggcctcagg
      421 atagtctttg gtgacactat attcgattcc tcaatctcaa ggatggttga aaccaccatc
      481 gaagacttga ggtgatccta tgcctgagat actagacgag ataatcaaca cctggcaggc
      541 ctcttttgag gcaaaacctt tcattccggt aattgaaaat gccggctcag tcagtgaagt
      601 tggtgatggt gttgccctag taagagggct ctctgatgcc ttcatcagcg aggtgttgag
      661 ttttgaaggt ggcatcgagg gtttcgtgct ctcgcttgct cctgattacg tcggagtcgt
      721 tttgctcggt gaagacaaat acgtcaggga aggtgacagg gtatcaagga ctggaagact
      781 tctctcaatt ccttacaccc aaaacctgat tggtcgtgta attgatcccc tcggcaatcc
      841 gattgatgga aaaccagcac caaaatcaat aaaatttctc ccgcttgaaa caaaagcccc
      901 atcagtaatc gagagggagc cagttcatca gccgatgcaa accggcatca agaccattga
      961 cgctcttttt ccgatcggca ggggacagcg tgagctcatc attggcgaca ggcaaaccgg
     1021 aaaaactgcc attgccattg atacaatcct gaaccagaag gacgaaaatg taatctgcat
     1081 ctatgttgga attgcacaga aatcgtccac catggcagga attgtcaaaa cactgcgtga
     1141 ttatggtgcg cttgattaca ccctcatcgt cacaaccctt gcagaccagt cgccagcaac
     1201 caggtacata gctccttatg tcggttgcgc aattggcgaa gagctgatgc gctccggcaa
     1261 gcacgtcctg ataatctatg atgacctctc aaagcatgcc gatgcctaca gggaacttgc
     1321 actgcttctg aggcgtccgc cagggcgtga ggcatatcca ggagacattt tctacctcca
     1381 ctcaaggctc ctcgagcgcg ctgcaaaact ccacaaggat cttggcggtg gttccatgac
     1441 cgccctgcca ataatcgaaa cacaggccgg cgatgtttcg cagtacatcc cgacaaacct
     1501 catatccatc accgacggtc agatatacct tgagaaagag cttttccacg agggtataag
     1561 gccagctgta aacgtcggtc tttccgtttc ccgtgtcggt ggaaacgccc agattccgac
     1621 catgaaaaag gtcggtggtt cactcaagct agatctgtca cagtacaatg acctcaaggc
     1681 attcgcccag tttggcgcag agctcgaccc ggtaacccaa aagaagctcc agaggggtga
     1741 aaagctcgtc gaactgctta aacaaccaca gtacagcccg atgcacgtgg gtcagcaggt
     1801 acttattctt ggcgcctact cgcttggcgc actggatgat ataagaaaag atagggtcca
     1861 ggagttcgag cgtgtccttg ttgaggaagc aaaaagattc aggcctgatc tccttaagga
     1921 gctctcaatg gcaccaaaac tgactgatgc gcacaaggaa ggaatatcca aacttgtgca
     1981 gtctgtatca tccacctttg ggagctgagc gtggcaacac taaggacact aaaacgacga
     2041 atagcctctg ttgattctgt agcgcacata acgcgctcaa tggagatgct ttctgtcgtc
     2101 aaaatttacc accttaggaa aaaggttgaa gaatcagtac cctatgttga aaaactcagg
     2161 gagcttctcg tatcgatagc aagccacacg tcaaccgacc tcaccgcgaa tcccctattt
     2221 attgcaagac ccatcgagaa agttctcatc atcccgctta cttcagacag gggtttttgc
     2281 ggcgcgttca actcaaatgt gatgtcgctg acaaaaagat actcggaaga gttgggcctg
     2341 aaatcaacat ttgccacaat aggcaaaaag gccaatgccg agctcacaaa gcaaggccac
     2401 gagatcagct tccagatgcc aggcatcctt gataagttgt catttaccca ggcccagata
     2461 ctgtccaaaa aaataaagga aatgtacata tcagacgaag tgcaggcagt ttcctttgtt
     2521 tatatggagt tccaatcagt acttcgccaa gaatcggtgg taagggaaat gctccctctt
     2581 gtcccaatca tgaaaggcca ttccatccca ctcgagacat tcatcttcaa gcccaatcca
     2641 acagccattc tggacacact catggacaac tatgttgacc tgttcgtcta caaagtcctt
     2701 cttgaggctg gcgcatcgga acaggcaatg agaaggatga gtatgcatag ggcaactgaa
     2761 aatgccgatg atttggggca aaagctcacg ataaaatacc aacgcaccag acaggctgca
     2821 atcaccaagg aattgaccga gataacctcc ggtgcagagg cgataaagga gagaaagagt
     2881 gtctaaagga aagctcatta gaatagaagg tccagttctg gacttttcct tccccaaggg
     2941 agagcttcca gacctttaca acgtggttgt ggttgaagac cctgcattgc cagaaaagct
     3001 cgtttgcgag gtccaccagg tcgtcaatgc cactgatgta cgttgcatag cccttggctc
     3061 aacagaagga ctccgcaggg ggatgatttg ccaggacaca ggcgctccaa tcaatgttcc
     3121 tgttggtgaa ggaacacttg gacgtgtttt caacgtcttt ggtaatccaa ttgacaaaaa
     3181 gggcgacgtg ccagcagttg aaaaaaggcc aattttccac aacccaccac aattctcaaa
     3241 aatcaatccg actgaatcca tctttgaaac cggcatcaag gtcattgacc ttcttgctcc
     3301 atttccaaag ggtggaaaaa tcggtctttt tggcggtgca ggtgtaggga aaaccgttct
     3361 tctccaggag ctcatacgca acgttgccta tgaacaccag ggagtgtcgg tattcgccgg
     3421 agtaggagag cgcaccaggg aaggcaatga gctcattctc gagatggaag cctctggagt
     3481 catcaaatcc accgcgttgg tcttcggcca gatgaacgag ccgccagggg tccgcatgag
     3541 ggtgcctttc accgctctga caatgtgcga gtatttccgt gacgacaaag gtcaggatgt
     3601 tcttctgttt gttgacaaca tattccgcta cgtccaggca ggctccgaag tctcagcctt
     3661 gctgggcagg atcccttccg cagtaggcta ccagtcaaca ctggccaccg aagttggctc
     3721 tcttgaggag agaattattt caacaaaaaa aggctccatc acctcagtcc aggcaatctt
     3781 cgttcccgcc gatgactaca cggacccagc cccggcaaac atcttcaccc accttgatgc
     3841 aacaatcaca cttgaccgcg cactggtcga gctcggaatc tatccggcag caaacccgct
     3901 tgattcttcc tcaaaaatgc ttgacccaag gatcgtcgga aaagagcact acgaggttgc
     3961 acaagaggtc aagagaatac tccagcacta cgaaaacctc aaagacatca ttgcgattct
     4021 cggaatgtcc gaactttctg aagaagacag aaaacttgtc tacagggcta g
//
LOCUS       DAOSRI010000160         4016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_20183_length_4016_cov_4.104014, whole genome shotgun sequence.
ACCESSION   DAOSRI010000160 DAOSRI010000000
VERSION     DAOSRI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20183_length_4016_cov_4.104014"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PK717_05525"
     CDS             <1..1034
                     /locus_tag="PK717_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOR47157.1"
                     /translation="WRKNRTKQQGGSVGVDLNRNYGYKWSEQGASGDPSDETYHGNKP
                     FSENETSIIKALSEEIPIIGCLTYHTFGELILYPWGYTKTKTSFDAKLAKLAEGMAKI
                     SGSPNDNDYKEKPLSDKYDYIPMQASGLYESSGDCTDFLFAEFGTPAFTIEMGETKDA
                     FAPPDTMIEPTINQVMEFNFYLMEESPKEFGILYGKVTDIFKNPVQAKITIPGLDQSL
                     KIDPGTGKFFRVLPKGKYLVSYGKNQKKLVELKDNFQKVDFIVESDQKVSLSGQILDQ
                     LGKPVKRKLQLLDASGNAVEGTAFTEKFVYTVPTGYYTIRIFRQDMGPQDFKVTLKSN
                     IDIDLVIRE"
     gene            complement(1024..1752)
                     /locus_tag="PK717_05530"
     CDS             complement(1024..1752)
                     /locus_tag="PK717_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47158.1"
                     /translation="MKKIPLLLVALSFLLSSCGRDPVPANEALASMRLSYETAIEVAR
                     QNLEIIGSDFRITRTNIDENTIVCNRPIPRSIVQAYGQQRAKKMMLGVCLIDQKLAPT
                     TPPGRLPIEGSPQFDYDDELLAKYREGLIFPKAMLDAVGDVPTEPILPFERKDAEEAL
                     AINYSTFATNLDKIISQAEKDGVDSGLIDAFKKAKNSLLSSLSRYQKAKDGATLDKDS
                     FDLAAGGFIYPIYEDIFGEKPPTP"
     gene            1839..3464
                     /locus_tag="PK717_05535"
     CDS             1839..3464
                     /locus_tag="PK717_05535"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOR47159.1"
                     /translation="MPKYVFVTGGVMSSLGKGIVSSSIGKIFEAMGFEVTILKFDPYL
                     NVDAGSQNPYQHGEVFVTDDGAETDLDLGHYERFLGKPLSKANNVTTGSLYQAVLAKE
                     RRGDFLGATVQIIPHLTQEIKSRIVQVGELTKSDIVIVEIGGTVGDIESQPFLEAIRQ
                     FGSEQKPEDVIYFHLTYVPLLRITGELKTKPTQHSVQELRRTGIKPHVIGCRSEVPIS
                     KDVRAKIALFCDVDLEHVYSLPDLDTVYKEPLYLDEQGLASQMAKLWNILPRQADLTN
                     WKKLVNTYMAPKNTVKIGMIGKYTKLSDAYLSVNEALKHAGIATENKVEIEWVDAETL
                     TTPGEVEKLSKYDGLLVPGGFGYRGIEGKIEAARYARENKVPYLGLCLGLQVMTIEFA
                     RHVCGLKNANSTEFVPETPEPVVDTMASQKFITDKGGTMRLGSQPCVIEEGTLAYSIY
                     NTNRIQERHRHRLEINNEYKTVLQEKGMVFSGINPTLNLVEIGEITNHPFMIGVQYHP
                     EFKSRFETPHPLFLAFIKAASGFRNKKPEQKLI"
     gene            3480..>4016
                     /locus_tag="PK717_05540"
     CDS             3480..>4016
                     /locus_tag="PK717_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOR47160.1"
                     /translation="MEQSYIFIKGARVHNLKNIDLTLPKNSLIVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFIGMMEKPDVDYIEGLSPAISIDQKTGSRNPRSTVGT
                     MTEIYDYLRLFMARVGTPHCPKCGKPIQKQTVQQMVDTLMSDYPGFRATILAPIARHR
                     KGEYKEQIEKMRKSGYTKM"
BASE COUNT         1251 a          910 c          912 g          943 t
ORIGIN      
        1 tttggagaaa aaacaggaca aaacagcagg gtgggtcagt aggcgtagac ctgaaccgca
       61 actatggata caaatggagt gaacagggag cttccggaga tccttctgac gaaacctacc
      121 atggcaacaa acctttttct gagaacgaaa cttcaatcat aaaagcactt tcggaagaaa
      181 tacccatcat cggctgtctt acgtaccaca cttttggaga gctgattctc tatccttggg
      241 gatacacaaa aacaaaaaca tcctttgatg ccaaactggc aaaactggcc gaaggaatgg
      301 cgaaaatctc cgggtcacca aacgacaacg attacaagga aaagccgctt tccgacaagt
      361 acgactacat accaatgcag gcttccggcc tctatgaatc atccggtgat tgcacagatt
      421 ttctttttgc cgagttcgga actcccgcat ttacgatcga aatgggcgag acaaaagacg
      481 ctttcgctcc acccgataca atgatagaac cgacaataaa ccaggtgatg gaattcaact
      541 tctatctcat ggaagaatca cccaaggaat tcggcatact ttatggcaag gtgaccgata
      601 ttttcaaaaa tcctgtccaa gcaaaaataa caatcccagg gctggatcaa tcactaaaaa
      661 tagacccagg aacaggaaaa ttcttcagag tgcttccaaa aggcaaatat ctggtgagtt
      721 acggaaaaaa tcagaaaaag cttgtagagc tcaaggacaa tttccaaaag gttgatttta
      781 ttgtggaatc agatcagaag gtatcattgt ctggccagat actggaccag ctgggcaaac
      841 cagtaaaaag aaaattgcag ttgcttgatg ccagtgggaa tgcagtggaa ggaactgcct
      901 tcacggaaaa atttgtctac acagtcccaa ccggatatta cacaataagg attttccgtc
      961 aggacatggg gccacaggat tttaaagtaa cgctcaaatc aaatattgat atagacttgg
     1021 tcatcaggga gtaggtggtt tttctccaaa tatatcttca taaattggat atatgaatcc
     1081 tccagctgca agatcgaatg aatccttgtc gagggtggcg ccatcttttg ccttctgata
     1141 cctggaaagc gagctcaaaa gggagttctt ggctttcttg aaggcatcaa taagcccgct
     1201 atcgacccca tctttttccg cctgtgagat tattttgtcg agattggtgg caaaagttga
     1261 ataattaatg gcaagcgctt cttcagcgtc ttttctctca aacggcagta tgggttctgt
     1321 ggggacatca ccaacagcat ccagcatggc ttttgggaag ataagtcctt ccctgtattt
     1381 ggccaggagc tcgtcgtcat agtcaaattg tggtgaacct tcaattggta accttccagg
     1441 tggcgttgtt ggtgcaagtt tctgatcaat gagacaaacg cccagcatca tcttttttgc
     1501 cctttgctga ccatatgcct gcacaatgga tcttgggata ggacggttgc aaacaatagt
     1561 gttttcatct atatttgtac gggttattct gaaatcggaa ccaatgatct caagattttg
     1621 ccttgcaacc tcgattgcgg tctcatacga gagcctcatt gaagcaagtg cttcgttggc
     1681 cggaacaggg tccctgccac aggacgagag caagaaagat agcgcaacaa gtagaagggg
     1741 aatcttcttc attctattaa gaatttaagt tcaaaaaagt cggcggtcaa ccgttgacca
     1801 ctttccggaa gaatgtataa aagtacgaga ggtgaaaaat gccaaaatat gtatttgtga
     1861 ccggcggagt tatgagctct ttaggaaagg gtatcgtttc ctcctccata ggcaagattt
     1921 ttgaggccat gggttttgag gtaacaatcc tcaaatttga tccttacctg aatgttgatg
     1981 ctggctcaca aaacccatac caacacgggg aggtcttcgt aaccgacgac ggtgccgaaa
     2041 ccgacctcga tcttggccat tacgagcgct ttcttgggaa acccctctca aaagccaaca
     2101 atgtaacaac aggctcactt tatcaggcag ttttggcaaa ggagcgcagg ggagacttct
     2161 tgggcgcaac agtccagata attccacacc tcacacaaga aataaaatca agaattgtcc
     2221 aggttggcga gctgacaaaa agcgacatag tcatagtaga aattggtgga acagtcggtg
     2281 acatcgaaag ccagccattt cttgaagcaa taagacagtt tggaagtgaa caaaaaccag
     2341 aggatgtgat atatttccac ctcacatatg tcccgttgct ccggataact ggagagctta
     2401 aaaccaaacc cacccagcat tctgtccagg agttgaggcg aaccggcata aaaccgcatg
     2461 taatcggatg caggagcgag gttccaatat caaaagatgt cagggcgaag atagccctgt
     2521 tctgcgatgt agacctggaa catgtctatt ctctccctga tctggacacc gtctacaaag
     2581 agccgcttta tcttgatgaa cagggacttg catcccagat ggcaaaactt tggaacatcc
     2641 ttccaagaca ggctgacctg accaactgga aaaaactagt aaacacctat atggcgccaa
     2701 aaaatacggt caaaatcggg atgataggca agtacacgaa actctccgat gcctaccttt
     2761 ccgtcaatga ggccctcaag cacgccggaa tagccacaga aaataaggtc gagattgagt
     2821 gggtggatgc tgaaacactg accacccccg gggaagtgga aaagctttca aaatacgatg
     2881 ggcttcttgt tccaggaggt ttcgggtaca ggggcatcga gggaaaaatt gaagcggcaa
     2941 gatacgcaag ggagaacaaa gtcccatacc ttgggttgtg ccttggcctc caggtcatga
     3001 caatcgaatt cgcaagacat gtttgtggcc tcaaaaatgc caattcgaca gagtttgttc
     3061 ccgagacccc agaaccagtt gtcgatacaa tggcaagcca gaaattcatc actgacaaag
     3121 gtgggaccat gaggctcggt tcccagccct gcgtaatcga ggaaggaaca cttgcttact
     3181 ccatctataa caccaataga atacaggaaa gacacaggca ccgcctcgaa atcaataatg
     3241 aatacaaaac cgtgcttcag gaaaagggga tggtgttctc cggcatcaat ccgaccctca
     3301 atcttgttga aattggcgaa ataacaaatc atccattcat gataggtgtc caatatcatc
     3361 cagagttcaa aagcaggttt gagacgccac accccttgtt cctggcattt ataaaggcag
     3421 cttctggatt caggaacaaa aagccggagc aaaaactcat ttagaaatca ggttttttaa
     3481 tggaacaatc atacatcttc atcaagggtg ctagggtgca caaccttaaa aacattgacc
     3541 tcaccctccc caaaaacagc ctaattgttg tcacaggtct ttctggttct ggaaagtcca
     3601 gtcttgcatt tgatacgctc tacgctgaag gacagcgcag atacgtcgag tcactctccg
     3661 cctatgcaag acaatttata gggatgatgg aaaaacccga tgtggactat attgaaggac
     3721 tttcgccagc gatttctatc gaccagaaaa caggatcaag aaacccacgt tcaacagttg
     3781 gcacaatgac agaaatttac gactacctaa ggctttttat ggccagggta ggtacaccac
     3841 attgtcccaa atgcggaaaa ccaatccaga agcaaactgt ccagcagatg gtagatacgc
     3901 tcatgagcga ttatcctgga ttcagggcca ccatacttgc accaatagca agacaccgca
     3961 agggtgaata caaagagcag atcgagaaga tgcgcaaatc aggatacaca aagatg
//
LOCUS       DAOSRI010000161         3883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21085_length_3883_cov_3.322362, whole genome shotgun sequence.
ACCESSION   DAOSRI010000161 DAOSRI010000000
VERSION     DAOSRI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21085_length_3883_cov_3.322362"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK717_05545"
     CDS             complement(<1..497)
                     /locus_tag="PK717_05545"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOR47161.1"
                     /translation="MARVLVINPGSTSTKVAVFDDEKEIAQENITHGNEELASFPKTS
                     DQAPFRLKAIKDFLSRHSIPEESIECVVGRGGPLKPIPAGTYIINDAMLVDLIESKRA
                     DHPSTLGGIIADMIRKERGIPAYIVDPVSVDEFEDFVRVAGFPECERVSLGHALNIRA
                     ACRRAV"
     gene            complement(596..1621)
                     /gene="mltG"
                     /locus_tag="PK717_05550"
     CDS             complement(596..1621)
                     /gene="mltG"
                     /locus_tag="PK717_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOR47162.1"
                     /translation="MMNRVLLWAGVFVLVLLVAVGGFYLWMQNAVATLYGNAEYPVTF
                     EVKVGSSPSQIAKLLADKGLISSEWVFLYEVRREEAGPKLQAGVFTFGEPLSMPQVIK
                     KLMTEGRLPPTKVLVPEGRTAREIGMLLSYLRSFDGEKYRELALNSKDSFQFRFSKTI
                     KGKSLEGYLYPDTYLLDDVSAEGLIKRQLKEFEQIFTSKFEKRCKELGLSVHELVTLA
                     SIIERESGTTEEMKNVSSVFWNRLKAGWKLESCVTVGYVLDKPSVILTLKELAVDSPY
                     NTYKYPGLPPGPVCNPGLSAIEAALWPAKTNYFFFVATGKGYNDFSETITEHNQKVAK
                     YLKNGLR"
     gene            complement(1611..2618)
                     /gene="rlmN"
                     /locus_tag="PK717_05555"
     CDS             complement(1611..2618)
                     /gene="rlmN"
                     /locus_tag="PK717_05555"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOR47163.1"
                     /translation="MNILELSREGLSKWFEGLGEKPFRTNQIIRWIWEKSVLDPEKMT
                     DLPENLRKIMKENFSKGPYVEKTAVSRDRSVKLLVRLEDGNAIESVLMRTAGHVTICV
                     STQVGCPLSCTFCQSGSTGLERNLEASEIIYQVALARPKPRNVVFMGVGEPMLNWTNF
                     KESVLRLQDEAGMSQRHMTVSTVGVPGMITRLAQELPRINLAISLHAPTQRLRERIMP
                     KAAKVMQIRKLIGEIKLYQGITGNRVTFEYVMLDGINDGLDSADALADITDGIASLIN
                     LIPYNPTDCASYKPSNPAKIRFFLERLRSQGRDATVRKSLGLGVDSACGQLRARRSND
                     E"
     gene            2718..3281
                     /locus_tag="PK717_05560"
     CDS             2718..3281
                     /locus_tag="PK717_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOR47164.1"
                     /translation="MRKFVLPVLIGVFCTTCLAATGCGTECETYSDAIGGTMKISSPA
                     FENGGAIPKKYTCDGENINPILVFSDLPDSTKSTVLICDDPDASSGHFTHWLIYDIHP
                     LALSISENEPKTRIMTNGSRQGQNDFGEIGWGGPCPPSGTHRYVFKLYALDKMLNLDL
                     GAKRKTVEVAMQGHIIAEAELTGTYGR"
     gene            complement(3285..3719)
                     /gene="ruvX"
                     /locus_tag="PK717_05565"
     CDS             complement(3285..3719)
                     /gene="ruvX"
                     /locus_tag="PK717_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOR47165.1"
                     /translation="MSIVALDLGTKNIGVAYSPDGVFSFPLPSIKRSDLPSDIEAIKK
                     VVLEKNANLLVVGYPTSEIESEWAKFVRGFAGKLKEALCINVALVNESLTSIEARNLG
                     HDMGKTDRQMREKKDAIEAQLILMRYLETFSQRMADIDPDSD"
BASE COUNT         1044 a          920 c          818 g         1101 t
ORIGIN      
        1 actgcccttc tacaggcagc cctgatgttg agagcgtggc ccagtgatac cctttcgcat
       61 tccgggaaac cggccaccct tacaaaatct tcaaattcat caaccgacac aggatcaaca
      121 atataggctg gaatacctct ttctttcctt atcatgtcgg caatgattcc gccaagggtg
      181 gatggatgat ctgctctctt tgattcaatg agatcaacaa gcattgcatc attgataata
      241 taagtgcctg ctggaattgg tttgagtggc ccacccctgc caacaacaca ttcaatgctt
      301 tcctctggaa tggagtgtct ggacaggaaa tcttttatgg ctttgagcct gaatggtgcc
      361 tggtctgatg tttttggaaa gctagcgagt tcctcattac catgtgtaat attctcctgc
      421 gctatctctt tttcgtcgtc gaagactgcc accttggttg aggtggagcc tgggttaatg
      481 actagtaccc ttgccatagg ctcctttcag gttgcggttt acttacttat tttatccttt
      541 ttagccagtc ggtcaatgaa gcaacacgcc gcaactgcaa taatctttta ctgttctatc
      601 tcagcccgtt cttgaggtat tttgcgacct tctgattatg ttctgtgatg gtctcagaga
      661 aatcgttgta gcctttgcca gtggcgacaa aaaagaaata atttgtcttg gctggccaga
      721 gtgccgcctc aatggccgac aagcctggat tgcacaccgg cccaggaggt aatccggggt
      781 atttgtaagt attgtagggt gaatcgactg caagctcttt caaagtaagg atcaccgaag
      841 gcttatcgag aacataaccg acagttacgc aagattcgag cttccagcct gctttcaacc
      901 tgttccagaa aaccgatgag acattcttca tttcctcagt agttccagat tccctctcga
      961 ttatcgaggc cagtgtcacc agctcatgga cactcagccc aagttccttg catctctttt
     1021 caaacttgct ggtaaatatt tgttcaaatt ctttcaactg gcgctttatg agtccttccg
     1081 cagagacatc atcaagaagg tacgtgtcag gataaagata accctcaagc gatttgcctt
     1141 ttatagtttt tgaaaatctg aactgaaagc tgtcttttga gtttagagca agctctctat
     1201 atttctcacc atcaaaactg cgcaggtatg agagcaacat tccaatctcc ctggctgttc
     1261 ttccctccgg aacgagaact tttgtgggtg ggagtctgcc ttctgtcatg agttttttta
     1321 tgacctgggg catcgaaagc ggctcgccaa aggtgaaaac accggcctgg agtttcgggc
     1381 cagcctcttc cctgcgaact tcataaagaa aaacccattc agagcttatg agtcctttat
     1441 ctgcaagtag ttttgctatc tgggaaggtg aggaaccaac tttaacttca aatgtaaccg
     1501 gatattcagc attgccataa agtgtagcaa cagcattttg catccataga taaaaaccac
     1561 ccactgcgac caacaaaacg aggacaaaaa cgcctgccca taaaaggact ctattcatca
     1621 ttgctccttc ttgcgcggag ctggccgcac gcagaatcaa ccccaagtcc aaggcttttt
     1681 ctcacagttg catctctccc ctgtgacctc aacctttcaa gaaaaaacct tatttttgct
     1741 gggtttgatg gtttgtagga tgcacaatct gtcgggttgt aagggatcag gtttataagg
     1801 gatgctatgc cgtccgtgat gtctgccagc gcatctgcac tgtccagccc atcattgata
     1861 ccatcaagca tcacgtattc aaaagtgacc ctgttgcccg ttatcccctg ataaagtttt
     1921 atctctccaa taagttttcg tatctgcatt acttttgcag cttttggcat gatcctttcc
     1981 ctgagtcttt gggttggggc gtgcagggat atggccagat tgattctcgg aagctcctgc
     2041 gcgagtcttg ttatcatacc aggtacacct acagttgaga cagtcatgtg tctctgtgac
     2101 ataccagctt catcctggag tctcaacacg gactctttga agtttgtcca attgagcatt
     2161 ggttccccaa cgcccatgaa aacaacattc cttggtttag gtctagcgag tgccacctga
     2221 tagatgattt ctgatgcctc aagatttctt tccagacctg tgcttcccga ctggcaaaat
     2281 gtgcaggaca atgggcaacc aacttgcgtt gaaacacaaa tcgtcacatg tcctgccgtt
     2341 ctcatgagga cagattcgat ggcatttcca tcctctagcc tcacaagcag cttgacggaa
     2401 cgatcacgtg atactgcagt cttttcaaca taaggcccct ttgaaaaatt ttccttcatt
     2461 atttttctca ggttttctgg caaatcagtc attttttcag gatcaaggac gctcttctcc
     2521 caaatccacc tgatgatttg atttgttctg aaaggctttt ccccaagacc ttcaaaccat
     2581 tttgacagcc cttccctgga aagttcaaga atgttcacag acaaatctta caaatagatt
     2641 ggggggctta caaggattca acaaaaacaa aacatcagac catttacaga gcaaaaccaa
     2701 ccaaataaaa tcacattatg aggaaatttg ttttgccagt attgattgga gttttttgta
     2761 ctacatgctt ggcagcaact ggttgtggaa cagaatgcga aacatattca gatgctatcg
     2821 ggggaacaat gaaaatatca agtcctgctt ttgaaaatgg tggtgcaata ccaaaaaaat
     2881 atacctgtga tggggaaaat atcaatccaa tcttggtttt ttcagatctt ccagacagca
     2941 caaaaagcac cgtcctcatc tgcgatgatc ccgatgcatc aagtgggcat ttcacgcatt
     3001 ggctcatcta tgatatccac ccacttgccc tttccataag cgaaaatgaa ccaaaaacaa
     3061 gaattatgac aaatggttca agacaggggc aaaacgattt tggagagatt ggctggggtg
     3121 ggccatgtcc tccatcgggc acgcacagat atgtgttcaa gctctatgca ctggacaaaa
     3181 tgctcaatct tgacttggga gcaaagagaa agaccgttga agtggctatg caaggtcaca
     3241 ttatcgctga agcagagctc actggtactt acggaagata ggtttcaatc agaatcggga
     3301 tcaatgtcgg ccattctctg ggaaaaggtc tccagatacc tcatgagaat gagttgagcc
     3361 tcgatagcat cctttttttc cctcatctgt ctgtctgttt tacccatatc atggccaaga
     3421 ttccttgctt caatagaggt tagactctcg ttcacaaggg caacattaat acaaagagcc
     3481 tctttgagtt tgccggcaaa acctcttaca aacttggccc actctgattc aatctcgctt
     3541 gttggatagc caacaacaag cagattggca tttttttcca aaaccacttt ttttatggct
     3601 tcaatatctg atgggagatc gctccgcttg attgaaggta ggggaaaaga aaacacaccg
     3661 tccggggaat atgcaactcc aatgtttttt gtgccaagat caagcgcaac tatcgacact
     3721 agatgaccct gaggcagtgg agtggacatt gaccagtgca ataagtgcaa acaacacact
     3781 tgtcatgatc aatgactgct ttgttttcag aaaggctgat agcttcttgt ggacaaaact
     3841 tgacgcaagt tccacagcca atgcaccaga atgaaacaag cat
//
LOCUS       DAOSRI010000162         3836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21402_length_3836_cov_4.148109, whole genome shotgun sequence.
ACCESSION   DAOSRI010000162 DAOSRI010000000
VERSION     DAOSRI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21402_length_3836_cov_4.148109"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK717_05570"
     CDS             complement(<1..658)
                     /locus_tag="PK717_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOR47166.1"
                     /translation="MGKIKVEELAVQLGVDPQEILESAKRLGESIKSVTSTLEDSTAA
                     KIRASFLLRRPASPPSQEQKPPVQTRTSLFDVDKKKTPVLKAATAEAVEAAKAAEAAL
                     ISSKEDKSTQKLEPITKIAKPEKKQAPAKKPIPSIILEEEEKAKHAKAAKPEAKIEEK
                     HELPKEEAQQIAIEAKTAEPKPATKKASHKEEIKEAAPVKPVAEDVAEKDVSHTAPST
                     P"
     gene            complement(645..908)
                     /locus_tag="PK717_05575"
     CDS             complement(645..908)
                     /locus_tag="PK717_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOR47167.1"
                     /translation="MPERMCVSCGKKSEKSSFIRISGRLELQSTGNTIGRSAYVCRNK
                     DCIQKLSNGKTRIGVSLKRNNIKKEELIKLSNEILTKVINGQD"
     gene            complement(908..1939)
                     /gene="nusA"
                     /locus_tag="PK717_05580"
     CDS             complement(908..1939)
                     /gene="nusA"
                     /locus_tag="PK717_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOR47168.1"
                     /translation="MKLTELFAALADIAREKDIEYSVLLDAIRLGIMAAYKKTYHANT
                     NIDVIVDDSQKKIQIIAKKIVVENVEDDTLEISPKDAKKISPEAEIGDTVEIEATPPD
                     FGRFAASTCKQIIMQRLKEAEKDKVIKEYSDKEKDMVFGEVSRVTGENVYFNLGKAEG
                     LLPKQEQIPGESYTPGRKFHCFVLRVDRSTREPLIILSRTHPNLLAKLMELEIPEIMQ
                     GVIEIIGIVREPGVRAKVAVHSNQSRVDPVGSCIGSKGVRINVISDELKGERIDVLRW
                     FEDPRLFVASSLSPAKVYSVQIFQNEKRAVVAVSADQFSLAIGKKGSNVRLASRLTGY
                     KIDMKVMEE"
     gene            complement(1948..2382)
                     /locus_tag="PK717_05585"
     CDS             complement(1948..2382)
                     /locus_tag="PK717_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47169.1"
                     /translation="MRSYLPKAREIILETLDKLGYNLVEIREIFGKEDYLVSIVIWKP
                     QGISIDDCVKITRAALPKLQQDPSLPDYTRLEVASPGIDRKLKNPSEFSVFKGRKATV
                     ATQEKSVTGTIGDSDENAVIMETDGILTSIPLSQIKSAKLVD"
     gene            2584..>3836
                     /locus_tag="PK717_05590"
     CDS             2584..>3836
                     /locus_tag="PK717_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR47170.1"
                     /translation="MSEHGDLRIAGLEISDILGIGETGITYRAKDAQDRDVVLKVLRW
                     DYTNQDERLQAELTLYRLTELAHPNLVQVYEVGTTEVGKIWYTRPFIQGDSLDVILQQ
                     VDAVGAQMLLLQVVDAIDFVHRNGFLHGNIHPENIMVAKGNPKGGRWETSRSFETPFI
                     VKVCDFGAVPPRESLRNGGLLPKDPSERTDIAALGKLVYEAYAGKLEEGMKPDLATIR
                     SHIPEPLTMVVARMLGLSQDEPFRSFQQIALGFSDLQPKLSSEACMERLSKNVLVQKD
                     SERRSFLMDMLADSQTKGHFVVIEGEEGSGKSVLVKEFASLAQFLGHQVSISSCTSRF
                     NPFEPLIRFIGDLEGGLATLNPVLLKNYQHIIDRVKGFSGLDAEKIPSTTSELFEGIQ
                     SFLTEVSHIQPLVLIIENIESSSAH"
BASE COUNT          925 a          820 c          801 g         1290 t
ORIGIN      
        1 gtggagtaga aggggctgtg tgagaaacat ctttttcggc aacatcttct gctactggtt
       61 taactggtgc agcctctttg atctcttcct tatgcgaagc ttttttagtc gccggttttg
      121 gctctgcagt ttttgcctct atggcaattt gctgggcttc ttcctttggc aactcatgct
      181 tttcttctat ttttgcctct ggttttgcag cttttgcgtg ttttgccttt tcttcttcct
      241 caagaattat tgatggaatg ggcttttttg ccggagcttg ttttttttct ggttttgcta
      301 tcttggttat tggttccagt ttttgggtag acttgtcctc ttttgagctg atgagggcgg
      361 cttctgcagc ttttgcagcc tctacagcct ctgcggtggc agccttgaga accggggttt
      421 ttttcttgtc cacatcaaac aaacttgttc tggtttgtac tggtggtttc tgttcctggg
      481 aagggggtga agcaggacgc ctcaaaagaa agcttgccct tattttggct gcagttgaat
      541 cttcgagtgt ggatgtcacg ctcttgatag attcaccgag tctttttgct gattccagga
      601 tttcctgcgg atcaacacca agctgaaccg ctaactcttc gaccttaatc ttgcccatta
      661 atcacctttg taagaatttc gttagaaagc tttattagtt cttctttttt tatattgttt
      721 ctcttcaagg acacccctat ccttgttttt ccattagaaa gcttttgtat acagtctttg
      781 tttctgcata cataggcgct tcttcctatg gtattacctg tactttggag ctctagcctg
      841 ccagaaatcc ttataaaact cgatttctcg ctttttttgc cacaactcac gcacattctt
      901 tccggcatta ctcctccatc accttcatgt cgattttgta gccggttaat cttgaagcaa
      961 gtcttacatt ggagcctttt ttgccgattg ccaatgaaaa ctggtctgca gaaactgcaa
     1021 ccacagccct cttctcgttt tggaatatct gcactgagta gacctttgct ggcgataaag
     1081 aagacgctac aaaaagcctt gggtcctcaa accacctgag aacatctatc ctttcaccct
     1141 taagctcgtc agaaatgacg tttattctta ctccctttga gccaatacac gaaccaacag
     1201 gatcaaccct ggactggttg gaatgtacgg ccaccttggc cctgacccct ggttccctga
     1261 cgataccaat tatctcgatg acaccttgca ttatttctgg aatttcaagc tccatcaatt
     1321 ttgcaagaag attgggatga gttcttgaca ggattatcaa gggttctctt gtcgatctgt
     1381 caaccctgag cacaaagcag tggaattttc tgcctggtgt gtaggattct cctgggattt
     1441 gctcttgttt tggaagaagc ccctcggctt tgccgaggtt gaaatagaca ttttctccgg
     1501 ttacccttga aacttcgccg aaaaccatgt ctttttcttt gtcggagtat tctttgatta
     1561 ctttatcttt ttccgcttcc ttcagtcttt gcatgattat ttgcttgcaa gttgatgcag
     1621 caaagcgacc aaagtctgga ggagtcgctt cgatttctac tgtatctcca atctcagctt
     1681 caggtgagat tttctttgca tcttttggtg aaatctcgag tgtgtcatct tcgacatttt
     1741 ccacaactat ttttttggct attatctgga ttttcttttg tgaatcatca acaataacat
     1801 ctatattggt atttgcatgg taagtttttt tgtaggcggc cataatacca agtcttatcg
     1861 catcaagcaa aactgaatat tcaatgtctt tttcacgggc tatatctgct agggcagcaa
     1921 aaagttctgt cagtttcatc tcttctccta gtccactagc tttgctgatt tgatttggga
     1981 aagtgggata cttgttaaaa tgccgtcagt ttccattata actgcgtttt cgtcagagtc
     2041 tcctatcgtt cccgtgaccg atttttcctg tgttgctact gttgccttcc gccctttgaa
     2101 aacagagaat tcggaaggat ttttaagttt tctgtcaatg cctggcgatg ccacctctaa
     2161 tcttgtgtaa tctggaaggg aaggatcttg ttggagcttt ggaagcgcag ctcttgttat
     2221 ttttacgcag tcatcaattg agatcccctg tggcttccat attacaatag aaaccaggta
     2281 atcttctttt ccaaaaattt cccttatttc aaccaggtta taacctagtt tgtcaagagt
     2341 ttccaggatt atttccctgg cttttggcaa ataactgcgc aatatcctaa tcctcctttg
     2401 tagattaatc caggtaataa tatcccataa tttgtgtttg cacaagtgtt ttttgttttt
     2461 tggactattt acggtttttc tatgtgcatt gaccaacttt atgagcttta tattgagtag
     2521 aaaggttgta aatatttact ccaagattat aattttatta acccgattaa atggaaggtg
     2581 ttcatgtctg agcatggcga tttaagaatt gccgggttgg agatttcaga tatccttgga
     2641 attggcgaga caggcatcac ttatagagca aaagatgccc aggatagaga tgttgtccta
     2701 aaagttttaa gatgggatta taccaaccaa gacgaaagat tacaggcaga actgacactt
     2761 tatagactca ccgagcttgc acatccaaat cttgtccagg tttatgaagt aggtaccact
     2821 gaagtaggca aaatctggta cacaagacca ttcatacagg gagatagcct tgatgttatt
     2881 ctccaacagg tggatgcagt cggggcccag atgcttttgc tgcaggtcgt cgatgcaatt
     2941 gactttgtcc atcgcaatgg ttttcttcat ggaaatattc atcccgaaaa tataatggtt
     3001 gcgaagggga acccaaaagg tggacggtgg gaaacctcca gatcatttga aacgcccttc
     3061 atcgtgaaag tttgtgattt tggggcagtt cctccaaggg aaagtctcag aaatggtggt
     3121 ctccttccaa aagacccaag tgagcgaacc gacatagcag cactgggcaa attggtatat
     3181 gaagcttatg ctggcaagct tgaagagggc atgaaacctg atctggcaac tatcaggtct
     3241 catatacccg aaccattgac tatggtggtc gcaagaatgc ttggtttgtc gcaggatgaa
     3301 ccattcaggt ctttccagca aatagctctt ggattttcag atcttcaacc aaagctctct
     3361 agtgaagcct gcatggagcg tctctcaaag aatgttcttg tccagaaaga ttctgagaga
     3421 agatccttcc tcatggatat gcttgcagat tcccaaacca aaggtcattt tgttgtaata
     3481 gaaggagaag aaggttctgg aaaatctgta cttgtaaaag aatttgcatc cctggcccaa
     3541 tttcttgggc atcaagtgtc catctcttcg tgcacttcca ggttcaatcc gtttgagcct
     3601 ttaataagat ttattggcga tctcgaaggt ggattagcaa cattgaatcc tgttttgctc
     3661 aagaactatc agcacataat tgatagagtg aaggggtttt cgggccttga tgctgaaaaa
     3721 ataccctcca ccacatcaga actttttgag ggaatacagt cttttttgac cgaagtttcg
     3781 catattcaac cgcttgttct tataattgag aatatagaat cttcctcggc tcacat
//
LOCUS       DAOSRI010000163         3821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21514_length_3821_cov_3.575943, whole genome shotgun sequence.
ACCESSION   DAOSRI010000163 DAOSRI010000000
VERSION     DAOSRI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21514_length_3821_cov_3.575943"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PK717_05595"
     CDS             <1..1580
                     /locus_tag="PK717_05595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOR47171.1"
                     /translation="QWVIRSVIKKIVDNMPEIGDGYLPNAVKKSMSLPSLRESYEKVH
                     FPDNFEQLKLCEKRLVLDEFLLMETALLLRRAEYRQQDAPIIKGAQKSSKDFLSKLPF
                     EPTIAQKRCCLEIAKDLESGRPMNRLLHGDVGSGKTVVGLSACSSAAKLGYQVAWIAP
                     TEILASQTYNVARKLLPFNTAYLSGSSKKMAREKIFELLSSGEPCVLFGTHALLEDWV
                     KFENLGLVVVDEQHRFGVLQRARLAKKGFCPHVLVMSATPIPRTLAMTIYGDLDVSVV
                     DEMPKGRATIETKVLTGAGSLAYKTALAEIEKGRQVYVVCPLVEESEKLSAKAATKQF
                     VELTESHFKGIPCGLLHGRMPAKEKDETMCKFRDRELKVLVSTTVIEVGIDVPNATVM
                     IIENAERFGLAQLHQLRGRVGRGEHKSYCFLIPSRPTVSLQVLERTANGLEVAEEDLK
                     LRGPGDIGGTMQSGLPMLASSRLITPSNFPLLSLAREIAEDIIKHDPELRFKENRELR
                     TGIEGKMAQRVGLAWVS"
     gene            1580..3481
                     /locus_tag="PK717_05600"
     CDS             1580..3481
                     /locus_tag="PK717_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR47172.1"
                     /translation="MGQIQVQNITKYFGVRAVLDGISFTLGGNDRLALVGANGTGKST
                     LIKIIMDQMEPDGGKVFKEPDKVIGYFAQDRSLDVSDVILFDQVRSAFKNIYETGRQL
                     EEISSEVAKDPHNSQLLAKFEKLQHQYEALGGYDIDHRIERILSGLGFSKEDWKRPVV
                     SLSGGERSRLQLATILATEPDTLVLDEPTNHLDLEATIWLEGFLQEFKGSVLIVSHDR
                     YLLDKVCNHTVILIDGKSFDFTGNFTQARAQYDLEREHSLEKLQEQQEFIERATRFIK
                     RFKGYGTEVAAKRARSMERRLERVVQVEALPREKKVQLNLESSGRTGEIVFRTKGLSK
                     SFGDRELFGELNLEMKRGQKIALMGPNGCGKTTFLKTVIGEEEPSGGEVWLGYNVSMA
                     YLEQELGSFNPEATVIEEIMNNSDLKPQEARDHLATFLFVGDEVSKPCKSLSGGEISR
                     LILAKLALIDANFIIFDEPTNHLDINARASLEDTIKNYSGSVLLVSHDRYFINNIGAT
                     IWAFDGNTIVDTRGNLEEYLQRKPEKQTDKSDQKIQQKQKKKDQGPSKNQIKSMQHKL
                     KNLEDKISMLESRKNDIEQSMSNPDFYSRGEQTKLDLDEYDKTKTQLAETENEWAELS
                     EELSAISAQ"
BASE COUNT         1118 a          869 c          950 g          884 t
ORIGIN      
        1 cacagtgggt cataagaagc gtaatcaaaa aaatcgtcga caacatgccg gagattggtg
       61 atggctacct tcctaatgcc gtaaagaaga gcatgtcttt gccatccctc cgtgaatcct
      121 acgagaaagt gcatttccct gacaactttg agcaactcaa actctgcgaa aaaaggctcg
      181 tgttggacga atttttgctc atggaaacgg ctctcctgct ccgcagggca gagtacaggc
      241 agcaggatgc tccaatcatc aagggtgcac aaaaatcttc aaaggatttc ctttcaaaac
      301 ttccgttcga gccgaccata gcccagaaga gatgttgtct ggaaatagca aaagacctgg
      361 agtcgggaag gccgatgaac aggctcctgc atggagatgt tggctctggg aagacggttg
      421 ttggtttgag tgcatgcagc tctgccgcaa agctgggcta ccaggttgca tggattgcgc
      481 cgacagaaat ccttgcaagc cagacctaca atgtcgccag aaaattactt ccgttcaaca
      541 ctgcgtacct ctccggttca tcaaaaaaaa tggcaaggga aaagatattt gagctgcttt
      601 cctctggaga gccatgcgtc ctctttggga cacatgcgct ccttgaggac tgggtgaagt
      661 ttgaaaacct cgggcttgtg gttgttgacg aacagcacag gtttggcgtc ctgcagaggg
      721 ccaggctagc aaaaaagggc ttctgcccgc atgtgctcgt catgagtgcc accccgatcc
      781 cgaggacgct tgccatgacg atctacggtg atctggacgt ctctgtggtt gatgaaatgc
      841 caaagggcag ggcaactata gaaacaaaag tgctcacagg tgcaggcagt ctcgcctaca
      901 aaacggccct cgccgaaata gaaaaaggca ggcaggtcta cgttgtctgc cctcttgtcg
      961 aagagtcaga aaaactctcc gcaaaagccg ccacaaagca gttcgttgag cttaccgagt
     1021 cacatttcaa aggcattccg tgcgggctgc tccatggcag gatgccggca aaagagaagg
     1081 atgaaacaat gtgcaagttc agggacaggg agttaaaggt tcttgtctcg acaaccgtca
     1141 ttgaggttgg aatagacgtc ccgaatgcaa cggtcatgat aattgaaaac gctgaaaggt
     1201 tcgggctggc ccagctccac cagttgaggg gcagggttgg aaggggcgag cacaaaagct
     1261 attgcttcct gataccgtca agaccgacag tttcactgca agttctggaa agaacagcaa
     1321 atggccttga ggttgctgag gaagacctca aattgcgcgg acccggagac attggcggaa
     1381 caatgcagtc tggactgccg atgcttgctt catcaagact catcactcct tcaaatttcc
     1441 cgcttctttc cttggcaagg gaaatcgcag aggatatcat taaacatgac ccggagttga
     1501 ggttcaaaga aaatagagaa cttaggacag gtattgaagg aaaaatggca caaagagtag
     1561 gacttgcctg ggtgagctga tggggcagat ccaggttcag aacataacaa aatattttgg
     1621 tgtgagggcc gttcttgatg gcatatcatt cacgcttggc ggcaacgaca ggctggcgct
     1681 tgttggcgca aatggcaccg gcaaatccac actgataaag ataatcatgg accagatgga
     1741 gccggacggc ggaaaggtct tcaaagaacc cgacaaggtc attggctatt ttgcacagga
     1801 caggtcactt gatgtctcgg acgtcatttt gtttgaccag gtcaggtcag ctttcaaaaa
     1861 catatatgaa accggtaggc agcttgaaga gatctcttca gaggttgcaa aagatcccca
     1921 caactcacag cttcttgcca agtttgaaaa actccagcac cagtatgaag cgcttggcgg
     1981 atacgacata gaccacagga tagaacgaat cctgtccggc cttggcttct caaaggaaga
     2041 ctggaaaagg cccgttgttt ccctctctgg cggtgaaagg tcacgcctgc aactggcgac
     2101 aatactggcc acagagcctg acacgctggt gctcgacgag cctacaaacc acctcgatct
     2161 tgaggcaacc atctggctcg aaggcttcct tcaggaattc aagggctcag tgctcattgt
     2221 ctcccatgac aggtaccttc tggacaaggt ttgcaaccat accgtgatac tcattgacgg
     2281 aaaatccttt gattttaccg gcaatttcac acaggcaagg gcccagtatg accttgagag
     2341 ggaacactct ctcgaaaaat tgcaggagca acaggaattc atcgagagag caacccgctt
     2401 tataaaaagg ttcaaggggt atggaacgga agtggccgcc aagagagcaa ggagcatgga
     2461 aaggcgcctt gagagggtcg tccaggtcga ggctttaccc cgggagaaaa aagtccaact
     2521 caaccttgaa tcatctggaa gaactggcga gatcgtcttc aggacaaaag gcttgtcgaa
     2581 atcttttgga gacagagagc tctttggtga gctcaatctt gaaatgaaaa gagggcagaa
     2641 gatagccctg atgggcccta atggttgcgg gaaaaccact ttcctgaaga cagtaattgg
     2701 cgaagaggag ccttcaggtg gagaggtgtg gcttggctac aatgtttcta tggcctatct
     2761 cgaacaggaa ctcggcagtt tcaatcccga agcaactgtt atcgaagaga taatgaataa
     2821 ttccgacctc aagccccagg aagccagaga ccatctggca acattcctct ttgtcggcga
     2881 cgaggtctca aaaccgtgca aatcactctc cggcggagag ataagcaggc ttattctggc
     2941 caagttagcc ctcattgacg ccaatttcat catctttgac gagcccacaa atcatctcga
     3001 cataaatgcc agggcttctc tggaagatac cataaaaaat tattccggga gcgtgctttt
     3061 ggtctcccat gatagatatt ttataaacaa catcggcgcc acaatatggg catttgacgg
     3121 aaacacaatt gttgatacaa ggggtaacct cgaggagtac ctgcagagaa aacctgaaaa
     3181 acagacagac aagagcgacc aaaaaatcca gcagaaacaa aagaaaaaag accagggccc
     3241 atcaaagaac cagataaaat caatgcagca caaacttaaa aaccttgaag acaagataag
     3301 catgctggaa tcaaggaaaa atgacattga acagtcaatg tcaaacccag atttctattc
     3361 tcggggagag cagacaaaac tcgatcttga tgagtatgac aagacaaaaa cacagcttgc
     3421 agaaaccgag aatgaatggg ccgaactttc tgaagagttg tccgcaattt ctgcccagta
     3481 gggtagtttt agcccaataa accaacatcc aaggcaattt cacaaaaaat tcattcttaa
     3541 cacaatttgt gaatttatta actttttaaa tcgaggttat tttctggttt tataaaacat
     3601 aagtttttgc ccatccttaa tttattcttc tttgttctta cggtgtaact tttttataaa
     3661 gtgtgttgtc tatgctcact gtaaagacat tgcttatcgt gtttacgctt atataatttg
     3721 caaaagtgtg caacttttgg aggtaagctt tgaacgatta ttctttcggt tacctgtttg
     3781 tgggcatctt cgccctggtt ggtctggcca tgggtcttat a
//
LOCUS       DAOSRI010000164         3818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21546_length_3818_cov_3.569227, whole genome shotgun sequence.
ACCESSION   DAOSRI010000164 DAOSRI010000000
VERSION     DAOSRI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21546_length_3818_cov_3.569227"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK717_05605"
     CDS             <1..416
                     /locus_tag="PK717_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOR47173.1"
                     /translation="NILGLSEEEDDEPILEAAVPATVDPLPKKSSQASQLASVLKKLT
                     VVVITPASFDEARKAVDSLKHGKPIMANFEDTDKEVARRFIDFVSGACYALDGVVEKI
                     GKNVFLFATSNVAVASERLRNWEEGSRSGALDDID"
     gene            436..3039
                     /gene="alaS"
                     /locus_tag="PK717_05610"
     CDS             436..3039
                     /gene="alaS"
                     /locus_tag="PK717_05610"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR47174.1"
                     /translation="MKSEDIRESFLSFFAERGHYRLEGASLIPSDPTVMFTLAGMVPL
                     KPYFTGERKPISRRLVSCQRCLRTSDIENVGKSPFHHTFFEMLGNFSIGDYFKQDAIP
                     WAWEFLTNIVGLNPEKLLVTVYPEDDESREIWKSIGVKEDRIQNKSDNFWMMGEVGPC
                     GPDSEIYYDWEAGEPIPFVGKEFDSDNKRFTELWNIVFTQYDAKPGGLREPLPAKNID
                     TGAGLERISAASQGVRSSLETDLFTPIISAISKIAPDNTDIQRRNLIADHIRACVFLV
                     SDGVFPSNDGRGYVLKRLIRRAALAGKKIGISESFLGELAMPVVENYSRAYPFLNERK
                     EAVVKILTNEEAQFRSTLAVGLQMLSDEIAAIKGKKAKIPGKVAFKLYDTYGFPLDLT
                     QEIAREEGSDVDIDGFNAELEAQRMRGRMAFTGQKEFEMATALIAFRDETGWCRFTGY
                     DSFSGTSEVLGILTKQGSENAATEGQNIQFTVKETPFYPEKGGQIGDTGKASTETCQL
                     EIIDTTSPVEGLILHKARITKGTLKVGQGIKLEINSERRSKIVKSHTATHLLHAGLRK
                     FLGEHAAQAGSWVGEEELRFDFTQMGEISRDDLLSIERWVNERIFENVPLNTAEMPIV
                     EAKKIGAVALFGEKYGDIVRVVTIPEISKELCGGCHVVSTGQISFFKIISESSIGANL
                     RRIEAKVGMKALLYVQEQEAKLFDVGELLACGQKQIKGKIEQVLDNLNDLQKKNRAMV
                     AQMAKAEAEKVTTQAKSMGVTQVIIARSDGFDMDGVRMVADFALEPFDHGVAAIISKN
                     GNSFVLGVISKGIPVTTLHCGKLVTKVASIVGFKGGGRPDLGQAGGIESSKFGQLTST
                     LETELHQILVK"
     gene            3036..>3818
                     /locus_tag="PK717_05615"
     CDS             3036..>3818
                     /locus_tag="PK717_05615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR47175.1"
                     /translation="MIERVSFGNTGLDVSRLCFGTLTIGQLQANLSMQDAVIVMSYAW
                     ERGINFFDTAELYESQNQIGMLPPSIRHNAIIATKSYAETPEKMRQSVEKSLREMKLD
                     VIPIYLLHEQETALTLKGHRGALDVLLEYKNKGLIRVVGVSTHTVEVTKLVRQIPELE
                     CVFSMFNVEGWGIKDGSIKDMEKELEWDYKTGKGTYLMKALAGGKLLHDAKRAIKYCI
                     DFPYAHSVAIGMKHPSEVDWNLELFEGKDPPPLKAIPRKMLVS"
BASE COUNT         1125 a          755 c          967 g          971 t
ORIGIN      
        1 aaaacatact cgggttatca gaagaggaag acgatgagcc gatacttgag gctgcagttc
       61 cagcaactgt ggaccccttg cctaaaaaat cctcccaagc atcacaactt gccagcgtac
      121 tcaagaagtt aacggtcgtt gtcataacac cagcctcgtt tgatgaggcc aggaaagccg
      181 ttgattcgct aaaacatggc aaacccatca tggccaattt cgaggacacc gataaggaag
      241 ttgcaaggcg ctttattgat tttgtctcag gggcctgcta cgctctggat ggcgttgttg
      301 agaagattgg aaagaacgtc tttttgtttg caacttccaa cgttgccgtt gcatcggagc
      361 gcttgaggaa ctgggaggag ggctctaggt caggggcact tgacgatata gactaaatcc
      421 agagcgattg cgacaatgaa atcagaagat atcagggaga gctttctctc cttttttgct
      481 gaaaggggcc attacaggct cgaaggtgcc tcattaattc cgtcagatcc aactgtcatg
      541 tttactcttg cgggaatggt accgttaaaa ccctatttta caggtgagcg caagccaatc
      601 tccagaaggt tggtcagttg ccagaggtgt ttgaggacta gtgatattga aaatgtaggc
      661 aaatcaccat tccaccacac tttttttgaa atgcttggca atttttcaat tggtgactac
      721 ttcaagcagg atgctatccc ttgggcatgg gaatttttga ccaatattgt gggccttaac
      781 ccagaaaaac tcttagtcac cgtctatcct gaggatgatg agagcagaga aatctggaaa
      841 tcgattggtg taaaagaaga cagaatacaa aacaaatcag acaacttctg gatgatgggt
      901 gaagttggcc catgcggccc cgactcggaa atatattatg attgggaagc tggggagcca
      961 ataccatttg ttggaaaaga attcgactct gataataaac gttttaccga gctgtggaat
     1021 attgttttta cgcaatacga tgcaaagcct ggtggtctca gggagccact gccagcaaaa
     1081 aatatcgaca ctggtgcggg actcgaaaga atatctgcgg cgtcgcaggg tgttagatca
     1141 agccttgaaa ctgacctttt tactccaata atttcagcga tttcaaagat tgcacccgac
     1201 aataccgata ttcagagaag aaatcttatt gcggaccata taagggcttg cgtttttctt
     1261 gtgtctgatg gtgtcttccc atcaaatgat ggcagaggtt atgttctcaa aaggctcatc
     1321 agaagggctg ctcttgccgg caaaaagata ggcatctcag agtcgttcct tggagagctc
     1381 gcaatgcctg ttgttgaaaa ctattctagg gcatatccat tcctcaatga acgcaaagaa
     1441 gcagtagtga aaatacttac aaatgaggaa gcgcaattca ggtcaacgct tgctgttggg
     1501 cttcagatgc tctccgatga aattgcggca attaaaggga aaaaagccaa gataccaggc
     1561 aaagtggcat tcaaacttta tgacacctat gggttcccac tcgatctcac acaggaaatt
     1621 gctcgtgaag aggggagcga tgtagacatt gatggtttta atgctgagct tgaggcgcag
     1681 agaatgcgcg gcaggatggc gtttaccggt caaaaagaat ttgaaatggc aacagcgctt
     1741 atagctttca gagatgaaac tggctggtgc cgtttcacag ggtacgatag tttcagcggc
     1801 acctcggaag tactaggtat tcttacaaaa cagggttcgg aaaatgcggc cacagagggc
     1861 caaaatattc agttcacggt caaagagacc ccgttttatc ctgaaaaggg tggtcagatt
     1921 ggcgatacag gcaaggcttc aactgagact tgtcagctcg agataattga tactacgtcg
     1981 cctgttgagg ggcttattct ccacaaagcc cgtatcacta agggaacact taaagttggt
     2041 cagggtatca aactggaaat caattctgaa cgaagatcaa aaatagttaa gtcccatacc
     2101 gcaacccacc ttctgcatgc tgggctccgc aaattccttg gtgaacacgc tgcacaggca
     2161 ggctcctggg ttggagaaga agaactcagg tttgatttta cccaaatggg tgaaatatcg
     2221 agggatgact tgctttcaat tgagcgttgg gtgaatgaaa gaatatttga aaatgtccct
     2281 ttgaatactg cagaaatgcc aatagtagag gccaaaaaga tcggtgcagt agcacttttt
     2341 ggagaaaaat acggtgatat tgtcagggtt gtgactatcc cggagatatc aaaggagctt
     2401 tgtggtggat gtcatgtagt ttcaaccggc cagataagtt tcttcaagat aatctctgaa
     2461 tcttccatag gtgccaacct gagaaggata gaagcaaaag ttggtatgaa agcgttgcta
     2521 tatgttcagg agcaggaagc caagctcttt gacgttggcg aattgcttgc atgtgggcaa
     2581 aagcagataa aaggcaaaat agagcaggtc ttggataatt taaatgatct gcagaagaag
     2641 aacagggcaa tggttgccca gatggccaag gctgaagctg aaaaagtgac aacccaggca
     2701 aagagtatgg gtgtaacaca ggtaatcatt gccaggtccg acgggtttga tatggatggc
     2761 gtaaggatgg ttgccgattt tgccctggaa ccgtttgatc atggggttgc tgcaattatc
     2821 tcaaagaatg gcaattcttt tgtattgggt gtgataagca agggcatccc tgtaacaaca
     2881 cttcactgtg gcaaactcgt aactaaagtt gcgagcatag ttggcttcaa gggtggcgga
     2941 agaccggatc ttggccaggc tggtggtatt gaatcttcaa aatttggtca gctgacatca
     3001 actctggaaa ccgagttgca tcagatattg gtaaaatgat tgaaagagtg tcttttggaa
     3061 acactggtct tgatgtttca aggctctgtt ttggaacact cacgataggt cagcttcaag
     3121 ccaacctctc catgcaggat gcagtcatag ttatgtccta tgcttgggag aggggaatca
     3181 atttttttga tactgccgag ctttatgaga gccagaacca gattggaatg ctcccgccaa
     3241 gcatcaggca caatgctatc atagcaacca aatcatacgc cgaaaccccc gaaaaaatga
     3301 ggcaatctgt tgagaaatct cttcgggaga tgaaacttga tgttatccca atataccttc
     3361 tccacgagca ggaaactgcg cttaccttaa agggccatag aggggctttg gatgtgcttc
     3421 ttgagtacaa aaataagggc ctcataaggg tggttggtgt ttcaacgcat actgttgaag
     3481 tcacaaaact tgtaaggcaa atacctgagc ttgagtgtgt cttttctatg tttaatgttg
     3541 agggatgggg aataaaagat ggctcgataa aagacatgga gaaagagctt gaatgggact
     3601 ataaaactgg caagggaaca tatctgatga aagctctggc tggtggcaag ctcctacatg
     3661 atgcaaaaag ggccataaaa tattgtattg atttcccata cgcgcactct gttgcaattg
     3721 gaatgaaaca cccgagcgag gtggattgga accttgaact ttttgaaggc aaggatccgc
     3781 cgccactcaa agcaatacca agaaaaatgc ttgtttca
//
LOCUS       DAOSRI010000165         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21654_length_3802_cov_3.927675, whole genome shotgun sequence.
ACCESSION   DAOSRI010000165 DAOSRI010000000
VERSION     DAOSRI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21654_length_3802_cov_3.927675"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PK717_05620"
     CDS             complement(<1..1123)
                     /locus_tag="PK717_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47176.1"
                     /translation="MIKKFLIFAQITVLLVLVSGCGGITVWGTKGGIPQRWYSANEGT
                     SNNVALVWDIGYETLALTSPVSDGEGIYIVTTTENGPGVVVLDEHSGKQAIAPVLLDE
                     AFTYELALDQNYLFVTSGSSLKCFYKRTMLENWTMEFDDEEVVSMFEHKDSLYGVTNI
                     GTAFCIHKETGVFKWKTDIAKDYVFRWVTLGERELFVTGGPKGFDYTHIFALSFMDGS
                     ILWHLEFDGPPDYPPQASAYTILVNSRARMYAYDTRSREEIWHYAITSLDGQPSILGC
                     NPCIYGDYVLVPSKSKIAKIGLKTGIEESRIIVPNTLQMRGIVASRDALFVALNGDPM
                     LYVFDMKTGLPARKIEGKRTILGMALCKGLVIQSQEAVTL"
     gene            complement(1355..3121)
                     /locus_tag="PK717_05625"
     CDS             complement(1355..3121)
                     /locus_tag="PK717_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR47177.1"
                     /translation="MNDRPTLISDGEKIKGIQRFSSKNSKFRYFTIALASVIIVGIFL
                     LVALNIPKSDILKVKITSEPKGAEVILDGISKGTTPLITRLKKGDVEIELSLAGYAPL
                     KSVATINENNQTINYKLAKSTGGTTILSYPDQARIYIDNEFLGLTPLQLKEEFKGKHV
                     IKAVLPGFATKEQEVDFDTDPSVYFDLQKKRVNLKIGSEPQGAELYIDEEHYGKTPWE
                     GQIETGTKKIRLAMEGHRMIKREVVITDDSQINYSFESKDISFDSQVDNDSAPGAKIY
                     ICIPNGETINKAIPPLFLGSTPLVASANTIGIYLTGNEKMPPYGLVFSNHPTYGATVS
                     SLPMDESGSILTNDVILTFPGYSSMDIIGQDLSSISYPSLINEKTRRPGTLDMNPRYI
                     VVGGSFVDRDNPSKKINIGQNIDKNDIIVSWDEKYMACYQPGQVKLFDLKQGSIIKTV
                     QAKNPEFSFTQDSTYLMVLSGGLMSKISCVAGQVESAACQSFGVLVPITDNILAVSNS
                     GKLSLLWDIASMRVTKFSSIGNGSFWEKYFEPRSIIQRSVSGSDKVVVLGEVFGLNCA
                     IAVNPKPALLNIWMPEENTRLP"
     gene            complement(3118..>3802)
                     /locus_tag="PK717_05630"
     CDS             complement(3118..>3802)
                     /locus_tag="PK717_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOR47178.1"
                     /translation="LAQSIKKSGVIQPIVVRRIRNGFELIAGERRLRAARLAGFEKIP
                     VVIREISDIDAAEFALVENIQREDLNPIDIARAMKRLREEFGYTQESIADGIKKSRPY
                     VANLLRLLDLPDIVQDAILKGTISEGHGRTLLSLKNDGLMLGMMQRIVTGQMSVRETE
                     KAVKKIQEETPAKKPQPREEEKTHEAVLAETALRETLGTKVRVLKDKIEIEYYGREDL
                     ERICEVIAK"
BASE COUNT          930 a          894 c          806 g         1172 t
ORIGIN      
        1 acaaagtgac cgcttcctgg gattggatta caagcccttt gcagagggcc attccaagta
       61 tggtcctttt gccttcaatt ttccttgctg gcaggccggt tttcatatca aaaacataga
      121 gcatcggatc accgtttaat gcaacaaaga gggcgtctct tgatgcaaca atgcccctca
      181 tctgaagggt gttcggaact atgattctcg actcttcaat tcccgtcttc aaaccaattt
      241 ttgcgatttt cgattttgac ggcacaagga cgtaatcgcc atatatacag ggattgcaac
      301 ctaatatact cggctggcca tcaagactgg ttatggcata gtgccatatc tcttctctgc
      361 tcctggtgtc ataggcatac atcctggccc ttgagttaac aagtatcgta taggctgatg
      421 cctgtggtgg atagtcaggg ggaccgtcaa actccaggtg ccacaaaatg ctgccatcca
      481 taaatgaaag tgcgaatata tgggtatagt caaacccttt tgggccacct gttacaaaca
      541 gctcgcgctc gccaagtgtg acccatctga aaacataatc ctttgcaata tcggttttcc
      601 acttaaaaac gcctgtttcc ttgtggatgc aaaaagctgt accaatattt gttacgccat
      661 agagcgaatc cttgtgttca aacatcgaga ccacttcttc gtcatcaaac tccatggtcc
      721 agttttccag cattgttctt ttgtagaaac atttgagact gctgccgctt gttacaaaaa
      781 ggtaattttg atcaagtgca agctcatagg taaaagcttc atcaagaagc accggagcaa
      841 ttgcctgctt tccgctgtgt tcatccagaa caacaactcc cggaccattt tcggtcgttg
      901 tgacaatgta tataccttcg ccatcagata ctggtgacgt gagcgcgagg gtttcataac
      961 cgatgtccca taccagagca acgttattac tggtgccctc gttggccgaa taccaccttt
     1021 ggggtatacc accctttgtg ccccagacag taattccccc gcaaccagaa accagcacaa
     1081 gtaaaacggt gatttgtgca aaaatcagaa actttttgat cattggccct aataatagaa
     1141 aattatactc tctggtaaag aacaatttgg ttttgtgcag tgtgtggaaa accctgtgga
     1201 caaattcatg ccattttgcc aagtgtcttt ggttttatga gacaattgtc ggtttctgtc
     1261 aatttaacaa tcaaaggaaa aataatctgc aagaagaatc attgcaaaaa aagttatcaa
     1321 catgtggaaa tctatgtgga tatcaaaaat gtgatcaggg taatcttgtg ttctcttcag
     1381 gcatccagat gtttagcaat gccggttttg ggttgacagc aatcgcacaa ttgagtccaa
     1441 aaacttctcc caggacgact accttgtcac tccctgaaac tgatctttgt attattgagc
     1501 gcggttcaaa atatttctcc caaaaactgc cgtttcctat cgatgaaaac tttgtgactc
     1561 tcattgaagc tatatcccac aacagcgaga gttttccaga atttgacacg gccagaatgt
     1621 tgtctgtgat tggcacaaga acaccaaaac tctggcaggc tgcgctttca acctgcccgg
     1681 ccacgcaaga gattttgctc ataagtccgc cggaaagcac catcaagtac gttgaatcct
     1741 gggtgaatga gaactctgga ttttttgcct gcactgtctt gattattgaa ccttgcttga
     1801 gatcgaagag ttttacttgt cctggctgat agcaggccat gtacttttcg tcccaagaca
     1861 caatgatgtc gttcttgtct atgttctgac caatatttat tttcttgctt gggttgtccc
     1921 tgtctacaaa agagcctcca acaactatgt atcttgggtt catatcaaga gtgccgggcc
     1981 gccttgtttt ttcgtttatg agggacggat agcttatgct tgaaaggtcc tggcctatga
     2041 tgtccattga agagtaacct ggaaaagtca aaatcacgtc gtttgttagt attgatccgg
     2101 attcatccat tggcaacgag gaaacagttg cgccatatgt tgggtgattg gagaacacaa
     2161 gcccatatgg tggcatcttt tcattgccgg tcaaatatat gccgattgtg tttgcgcttg
     2221 caaccagcgg tgtggaacca agaaaaagtg gtggtatggc tttgtttatt gtctccccat
     2281 ttgggatgca gatatagatt tttgcaccag gtgcagagtc gttgtccacc tgtgaatcaa
     2341 acgatatgtc cttggattca aaagaataat ttatttgaga atcgtcggtt atgacgacct
     2401 ctctctttat cattctgtgg ccttccatgg ccagtctgat cttttttgtt cctgtttcaa
     2461 tctggccttc ccatggggtc tttccataat gctcttcgtc gatgtacagt tctgcgccct
     2521 gtggctcgga gccaatcttg aggtttaccc tcttcttctg gaggtcaaaa tatacgctcg
     2581 gatcagtgtc gaaatcaact tcttgctctt ttgttgcaaa ccctggcaaa acagctttta
     2641 tcacatgctt ccccttaaac tcttccttta gttgcaaggg agttaggcca agaaactcat
     2701 tatcaatata tattctcgcc tgatccggat atgagagaat agttgttcct ccagtggatt
     2761 tggcaagctt atagtttatg gtctggttgt tttcattaat tgtggctaca gacttcagtg
     2821 gtgcataacc ggccagcgag agctcgattt ctacatcgcc tttttttagt ctggttatca
     2881 gtggtgttgt gccctttgaa atgccatcca gtataacctc agcacctttt ggttctgagg
     2941 ttatcttgac cttcagaata tcactctttg ggatgttgag tgccaccagg agaaaaatgc
     3001 caacgatgat tacagatgcg agtgctatcg taaaatacct gaacttggag ttttttgaag
     3061 agaacctttg gatgcccttt attttctcgc catccgatat cagtgttggt ctatcattca
     3121 ttttgcaatg acctcacata ttctttccag gtcttcccta ccataatact caatctctat
     3181 cttgtctttt aagaccctta ccttggtccc caaggtttcc ctgagtgcag tttctgcaag
     3241 gactgcttca tgggttttct cttcctcccg cggctggggt ttctttgccg gggtttcttc
     3301 ctgtattttc tttacagcct tttctgtttc ccttacagac atctggccag taacgatcct
     3361 ttgcatcatg cccagcatga gcccatcatt tttgagggag aggagcgttc ttccatggcc
     3421 ctctgatatt gttcccttga gtattgcgtc ctggacgata tcgggaagat cgaggagcct
     3481 caaaagattt gcaacgtaag gtctgctctt cttgatgccg tctgcgatcg actcctgtgt
     3541 gtagccaaat tcctcgcgga gcctcttcat ggccctggct atatcgatcg gattgagatc
     3601 ctccctctgg atgttttcca caagggcaaa ctctgccgca tcgatgtccg atatctccct
     3661 tatgacaacg gggattttct caaaacccgc cagtcttgca gccctaagcc ttctttcacc
     3721 tgcaatgagc tcaaagccgt tcctgatcct tctgacgaca ataggctgga ttacgccaga
     3781 tttctttatt gactgcgcca gc
//
LOCUS       DAOSRI010000166         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21721_length_3792_cov_3.906877, whole genome shotgun sequence.
ACCESSION   DAOSRI010000166 DAOSRI010000000
VERSION     DAOSRI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21721_length_3792_cov_3.906877"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..721)
                     /gene="mscL"
                     /locus_tag="PK717_05635"
     CDS             complement(272..721)
                     /gene="mscL"
                     /locus_tag="PK717_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOR47179.1"
                     /translation="MFKEFKEFALKGNVIDLAVGVVIGAAFGAIVNSLVNDIIMPPIG
                     FLLGRIDFSNMYLLISSGKTPPPYASLAEAKAAGAVTWNFGLFINALISFLIISFSIF
                     IVVKMINRLRKEKPAEKPAPTQKECPFCFTLIPIKATKCPHCTSEIK"
     gene            complement(774..1826)
                     /locus_tag="PK717_05640"
     CDS             complement(774..1826)
                     /locus_tag="PK717_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47180.1"
                     /translation="MKGIDEKIEFLLKNAGPSIKYRTRRDILGKGHDAEMKRLQEEIL
                     SEKLITKVLSLQGESGGFGDRLHTAPSNNRFNALEVATKFLIEKGIEYSHPAIEKAAR
                     YAKEMALKNPLEMSNLADESDGGKTSASEMIIARVLVQCGKENEKVVKKQIKTSLESF
                     RMAKNISNWNEVTEQWRGKFVFRKGFCCPCYYDLFILANTSSWRNEENLSMLVQSSEN
                     LLLLDSPQSKIYELHKNTAYSPAWSITDGLVRNFDSLDKDSAFCWFQRMEHLAKCGLA
                     RRIKYLEKQTQKLILSFDQTDDWISSVMSGHSFTTWSAYVGSSLETGKTKSMLRNDFL
                     FRSLLILKYSKDCLKT"
     gene            2118..3401
                     /locus_tag="PK717_05645"
     CDS             2118..3401
                     /locus_tag="PK717_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47181.1"
                     /translation="MKKVLATIATVALALGMFTTGMVGAAQEEDKGMGGMFMGTVQQI
                     GQDNLTMKMAFSEFMTAKVILSPDCKYLDLTSQPEEGKEPPALTKADIKVGDKIVCKG
                     KIAREEGKDAVFTALTVGKVKEFPKPRPPQDDKGDRKPQIMGVFVSATDTAFTMKAPD
                     SDKTGTFNYNEKTKFVRIAEKDGKKERQNITIKDIKSGDKLVVVAVPLKDKEGDDKER
                     VMTAVIVTVVDKFPELPPPGEGRREERRPTAIGSVKSFDGKNLTVELWKNKDIKTAGF
                     TVDEKTKVLYFPKQDGDKKPEPVEKKLEDLKPGQKVMVDFRPNEDTKPEDGVIAGLAI
                     RIVLIDEFPPEVAMGEIVEISASSVTINARIDKENTEKVTIQITSDTKFVIMKRGEKP
                     QEAKLSDFAKGDKVAVDYRDGKALVIKKVMVPEKK"
     gene            3499..>3792
                     /locus_tag="PK717_05650"
     CDS             3499..>3792
                     /locus_tag="PK717_05650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR47182.1"
                     /translation="MIGMNNDGLLGRFDAFWEDKARIEYGFKVPEQWFLDEAEKMYPD
                     GLRVLDLGCGNGRNAVPLARMGCKVTGVDISKVALSLLKEKSQKKGVCVDVVQS"
BASE COUNT         1137 a          760 c          864 g         1031 t
ORIGIN      
        1 gcgttaatgg caatcctcct ttctttttgc cagactttgc tggcccccaa tggctcctca
       61 agatcgacat tggggattat caaaagaaag gcaaacagca aaaacgaaga tatctttaaa
      121 taatgtaaat tgtctaaaat ttacttgata attgttttta taaaagtgac aaaaacttat
      181 ctggagagag ttatttttga cgaacgcaat tagcgtaaaa tgtcagatgc accggaaacc
      241 tccggtgcgt cctattaaaa ataagtctga attacttgat ttcagaagtg cagtgcgggc
      301 attttgtggc ttttataggg atgagggtaa agcagaatgg gcattctttt tgggttggtg
      361 ctggtttttc agccggcttc tctttcctga gcctgttgat catcttgacc acgatgaaga
      421 ttgagaaaga tattatcagg aaagatatga gcgcatttat aaagagcccg aagttccatg
      481 tcacggcgcc agcagctttt gcctctgcaa gtgaagcata tggcggaggg gtcttgcctg
      541 atgaaataag aagatacatg tttgaaaaat ctatcctgcc aagtaaaaag cctataggag
      601 gcatgattat gtcgtttaca agtgaattga caattgcacc aaatgccgct cctattacga
      661 cgccgactgc cagatcaatg acgtttccct tgagtgcgaa ttctttaaat tctttgaaca
      721 tctctcacct cctcttcaaa tttagcatgg ctgtcaatct ggccaaccaa agtttatgtt
      781 ttcagacaat cttttgaata tttcaatatc agcaacgacc tgaaaaggaa gtcatttctg
      841 agcattgatt tggtttttcc tgtttccagc gatgagccaa catatgctga ccaggtagtg
      901 aaggaatggc cactcatgac tgatgaaatc cagtcatcgg tttggtcaaa acttaaaatg
      961 agtttttggg tttgcttttc aagatacttg atccttcttg ctagcccaca ctttgcaagg
     1021 tgttccattc tctggaacca gcagaatgca gaatctttgt ccaaactgtc aaaatttctg
     1081 acgagtccat cagtaatcga ccaagctggt gaataggcag tgtttttatg aagctcatat
     1141 atttttgatt gtggagagtc cagcaacaag aggttttctg atgactggac cagcattgaa
     1201 aggttctctt cattcctcca agaagatgtg tttgcaagga tgaaaaggtc atagtaacag
     1261 gggcaacaaa agcctttcct gaaaacaaat ttacccctcc attgttctgt aacctcattc
     1321 cagttggaaa tgttctttgc cattctaaat gattcgagcg aagttttgat ctgcttcttg
     1381 acgacttttt cgttctcttt tccacattgc actagcaccc tggcaattat catttcgctt
     1441 gcacttgttt ttccaccatc gctttcatca gccaaattgc tcatctcaag agggttttta
     1501 agggccatct cttttgcata tctggccgct ttttctattg ccggatggct gtattcgatg
     1561 cccttctcaa tgagaaattt tgttgcgacc tccagtgcgt tgaagcgatt gtttgagggt
     1621 gcggtatgca acctatctcc aaaaccgcca ctttctccct ggagtgacaa aacctttgta
     1681 ataagttttt ctgaaagaat ttcttcctgg agtcttttca tttcggcatc atgccctttg
     1741 cccaaaatat ctcttcttgt tctatatttt attgaagggc cggcattttt gagcagaaat
     1801 tcaattttct catcaatacc tttcattttt gcctcctgaa acaacaattt acttgccatt
     1861 tatatactaa tttttaagta aatgcattat ttatacattt gtttacattt gaaatatagc
     1921 atcagaaaca gttatcatcc tctttttaaa gtggtaataa tagccagaac taataaactg
     1981 ctgtgttcca tttcaaatga ttaaaccaaa ctttctgaaa ttgaaacaaa ccactataat
     2041 tgcagaattt tttatactaa ataacttttt actatgccct atcgtattat gaggcatgaa
     2101 aggaaggagg cactaagatg aaaaaggttt tagcaacaat agcaacagta gcgctggctc
     2161 tgggaatgtt taccaccgga atggtaggag ctgctcagga agaagataag ggaatgggtg
     2221 gcatgtttat gggaactgtc cagcagatcg gacaggataa tctcaccatg aagatggcat
     2281 tctcggagtt tatgaccgca aaagtaattc tctcgcctga ttgcaagtat ttagatctta
     2341 caagccagcc agaggaaggc aaagagccgc cagcattgac caaggcagac attaaagttg
     2401 gagacaagat tgtctgcaag ggcaagatag caagagaaga aggcaaagac gcagtattca
     2461 cagctctaac tgtaggcaag gtcaaagagt tcccaaagcc aaggccaccg caagatgaca
     2521 agggcgatag gaaaccccag atcatgggcg tgtttgtgtc tgcaaccgac acagctttca
     2581 caatgaaggc gccagattct gacaagacag ggactttcaa ctacaacgaa aaaaccaaat
     2641 ttgtccgtat cgcggaaaaa gatggcaaga aagaacgcca gaatattaca atcaaggaca
     2701 taaagtcagg cgacaagctc gttgttgttg cagtaccact aaaggacaaa gagggcgatg
     2761 acaaagagcg tgtaatgacc gccgttatcg tcaccgttgt tgacaagttc ccggaactcc
     2821 cgccaccagg agaaggcaga cgcgaagagc gcaggccaac ggccattggt tcagtaaaat
     2881 cctttgatgg gaagaacctc actgttgagc tctggaagaa caaggacata aagacagccg
     2941 gattcactgt tgatgaaaag accaaagttc tttacttccc aaaacaggat ggagacaaga
     3001 agccggagcc agtcgagaag aagcttgaag acctgaagcc aggccagaaa gtcatggttg
     3061 attttaggcc gaacgaagac accaagccgg aagatggtgt gattgcaggg ttggccatca
     3121 ggatagttct gattgacgag ttccctccag aagttgcaat gggcgaaatt gtggagataa
     3181 gcgcatcatc ggttacaatc aatgccagga ttgacaagga aaacaccgaa aaggtgacaa
     3241 tccagatcac atcagacaca aagttcgtca tcatgaagag gggcgagaaa ccccaggaag
     3301 caaagctctc agattttgca aagggcgaca aggtggccgt tgactaccgc gatggcaagg
     3361 cgcttgtcat aaagaaagtc atggtccccg aaaagaagtg atttgacagg accagtagtt
     3421 agcatcataa aaaaggcccg gagctaaccc cgggcctttt ttatttgaac tgatgaaaaa
     3481 acaggagaat gggctaaaat gattggcatg aacaatgatg gtcttttggg aaggtttgat
     3541 gcgttctggg aagacaaggc aagaatagaa tatggcttca aagtgccgga gcagtggttt
     3601 cttgatgagg cagaaaaaat gtatcctgat ggtctcagag tactggacct tggctgtggc
     3661 aacggaagaa atgctgttcc tctggccagg atggggtgta aggtgactgg tgtggacata
     3721 tcaaaggttg cgctttccct tttgaaagag aagtcccaaa agaagggagt atgtgtggat
     3781 gttgtccaat ct
//
LOCUS       DAOSRI010000167         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21840_length_3777_cov_3.744224, whole genome shotgun sequence.
ACCESSION   DAOSRI010000167 DAOSRI010000000
VERSION     DAOSRI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21840_length_3777_cov_3.744224"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..928
                     /locus_tag="PK717_05655"
     CDS             245..928
                     /locus_tag="PK717_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOR47183.1"
                     /translation="MEFEILGNQLPVVVCKMKKGEKMVSESGGMSWMTTGMAMQTTMK
                     GGLLGALTRGISGESAFVNTFESTGENDEIAFASSFPGQIVHMKLEEGQYIIAQRGAF
                     LAGSENVKLEVYFRKKFWAGMFGGEGFVLNKISGPGDIFLEIDGSLIEKTLAPGEMLK
                     IDTGHCAILEPTINFDVQMLKGFKNIFFGGEGLFLAVVTGPGKIWIQTLTVAELAKDI
                     IPFIPTKSS"
     gene            1134..1682
                     /gene="pyrR"
                     /locus_tag="PK717_05660"
     CDS             1134..1682
                     /gene="pyrR"
                     /locus_tag="PK717_05660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOR47184.1"
                     /translation="MDLKVKAILLEKEDMKRAITRMAHEIIENNHGIKDLALVGIHRR
                     GVPLAKRIGQRIEEIEGKLPLVGELDITLYRDDLSMLYEAPQVHKTKVDFNIKDKVVV
                     LIDDVLFTGRTVRAAMDALTDLGRPRKIQLAVMVDRGHRELPIRADYIGKNVPTSINE
                     IVEVLLEETDGQDKVILAQKAI"
     gene            1738..2664
                     /locus_tag="PK717_05665"
     CDS             1738..2664
                     /locus_tag="PK717_05665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOR47185.1"
                     /translation="MGLKQKDLLGLKDLSREEISLILDTAVSFKEVLGRDIKKVPVLR
                     GKTVVNLFYEPSTRTRTSFELASKMLSADVVNIATQTSAVQKGECLKDTVLTLTSLGA
                     DYIVIRTSMCGAPHFISTFFQGHVLNAGDGINEHPTQALLDMYTIKEKLGKIEGLKVL
                     ILGDILHSRVARSNMFGLVKMGAEVGVCGPPTLIPKDVAKLGVKVHLDLDEALPNYDI
                     VNVLRVQLERQRQGMFASAHEYFEHYGLTTQRLEKNKHLLVMHPGPMNRGVEISPDVA
                     ESENAVIETQVTNGVAVRMAVLYLLLGGESHE"
     gene            2657..>3777
                     /locus_tag="PK717_05670"
     CDS             2657..>3777
                     /locus_tag="PK717_05670"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOR47186.1"
                     /translation="MNSKVLKGGKIVDVVSEKIFEADVKIEDGKIAEVGQNLTAEETI
                     DCKGMLVSPGLIDLHVHLREPGFEHKETIEAGTKAAAAGGFTRIYAMPNTEPPCDNEA
                     SAIYIRQQADRLGLIRVEPVGSISQGRKGEFLAEMGGLARAGVKMVTDDGNPVSSASL
                     MLLALKYSKAFGMLVADHCEDKSISANGICNEGYFSTLYGLQAWPKSAEETIVSRDII
                     LAGETDSRIHIMHISTKFSAQLIAQAKSMGIKATCEVTPHHLTLTDELLGDYNTNWKV
                     NPPLRGKEDIEALRKALADGIIDCIATDHAPHAREDKEVEINIAPPGISGLETAVGQL
                     FTELVSKGVISPEKLLSRMTVGPANVVGLEIPEIKAGKPA"
BASE COUNT         1169 a          851 c          899 g          858 t
ORIGIN      
        1 atcatcgata ttccgacaaa gtacatcaca accatcaaag cgcccaatac tccagcaaca
       61 gttaactgtt ttatggtaat cttcataaaa tctccttttt attagggttt ggcttggtcg
      121 cccaatgcca accggtggag attttatttc attagcaaaa atgagcaaga ccgcaagtag
      181 tgacatattt gcgaaaaatt tgtagaatta atacgaaatc aaaaataaca aggaggtcat
      241 gtttatggaa ttcgagattt tgggcaatca acttccagta gtggtttgta aaatgaaaaa
      301 gggcgaaaaa atggtctctg aatctggcgg aatgagctgg atgacaactg ggatggccat
      361 gcagacaaca atgaagggtg gtctgcttgg tgcattgacc cgaggcatat caggagaatc
      421 tgccttcgtg aatacttttg aatcaacggg cgaaaatgat gaaatcgctt ttgcatcctc
      481 attcccagga caaatcgtac acatgaaact tgaggaaggg caatatatca tagctcagag
      541 gggcgccttt cttgctggtt ccgagaacgt caagctcgaa gtatacttca gaaagaaatt
      601 ctgggcaggc atgtttggtg gagaaggttt tgtcctcaac aaaatctctg ggccaggaga
      661 tatcttccta gaaatagacg gctcattgat agaaaaaacg ctcgcacctg gggaaatgct
      721 caaaatagat accggccatt gcgccattct tgagccaaca atcaatttcg atgtccagat
      781 gctcaaaggt ttcaaaaaca tcttctttgg aggcgaaggt ttattccttg ccgttgtaac
      841 cggtcctgga aaaatatgga tacagacact cacggtggcc gagcttgcca aggacatcat
      901 tccatttatc ccgaccaaga gcagctgaag cctacatatt aaaaaaatag ccatctgcta
      961 acattttttt ataattgaaa aacctgtttc ggaatcaaat tcaggaaaat acataaatat
     1021 ctatggtatt aaattgtaaa taggtattca caaccttatg cacaaatgtt aacaacatgg
     1081 caaaccaaaa acattattga cacaagaaat ccaaaattta tactaaacaa acaatggacc
     1141 tcaaagtcaa agcgatactg ctcgaaaaag aagacatgaa acgggctatc acaagaatgg
     1201 cccacgagat aatcgagaac aaccatggca taaaagacct ggccctggta ggtatccatc
     1261 gcaggggagt tccgcttgca aaacgaattg gccagaggat tgaagaaatt gagggaaaac
     1321 ttccactagt aggcgagctt gacatcacgc tctacaggga cgatctttcg atgctctacg
     1381 aagcgccaca agttcacaaa acaaaagttg atttcaacat aaaagacaaa gttgttgttc
     1441 tgattgatga tgtcctcttt acaggtagaa ctgttagagc cgccatggat gcgctgacag
     1501 atcttggaag accaagaaaa atccagctgg cagtcatggt tgacaggggg cacagggaac
     1561 tgcccatcag ggccgattac atcggaaaaa atgttcccac atccataaac gagattgttg
     1621 aagtgctcct cgaggaaacg gatgggcagg acaaagtaat cctagcccaa aaggcaatat
     1681 gataaaccgt ttattcagcc cctggcctcc ggtcaggggc ttttttttgg aggttgaatg
     1741 ggtctgaaac aaaaagattt gcttggtctg aaggacctgt ccagggaaga gatctcgctc
     1801 attctggaca cggccgtgag tttcaaggaa gtcctcggca gggacatcaa gaaggtccca
     1861 gtattgagag gcaaaaccgt cgtcaatctt ttctatgagc catccacaag gaccagaaca
     1921 agttttgagc tcgcttcaaa gatgctctcc gctgatgttg tcaatatcgc aacccagaca
     1981 tcggcagtac agaaaggtga atgtttaaag gacactgtcc tgacactcac ttctcttggg
     2041 gcagactaca tagtcatcag gacaagcatg tgtggagcac cacactttat atccaccttc
     2101 ttccagggac atgtcctgaa cgcaggcgat ggcatcaatg agcacccgac acaagcactc
     2161 ctggacatgt acacaatcaa ggaaaaactt ggcaagatag agggcctcaa ggtcctgatc
     2221 cttggtgata tccttcattc cagggtcgca agatcaaaca tgttcgggct cgtaaaaatg
     2281 ggcgccgagg taggagtatg cgggccgcca acacttattc caaaagatgt tgcaaaactt
     2341 ggagtaaagg tccatcttga cctcgatgag gcgctaccaa actatgatat agtcaacgtc
     2401 ctcagggtcc agcttgaaag gcagcgtcag ggaatgtttg cttctgctca cgaatacttc
     2461 gaacactatg gactgacaac acagaggctc gaaaaaaaca agcacctgct tgtcatgcat
     2521 cctggaccaa tgaacagggg cgtcgagatt tcgccagatg tcgccgagtc agaaaatgcg
     2581 gtcatcgaaa cacaggtaac aaacggagtg gctgtccgca tggcggtgct ttatcttctg
     2641 cttggaggtg agtctcatga atagcaaggt actcaaaggt ggaaaaatcg ttgatgtggt
     2701 ttcggagaaa atcttcgaag ccgatgtcaa aatcgaggac ggcaaaatag ctgaagtcgg
     2761 acaaaacctg actgcagaag aaaccatcga ctgcaaggga atgcttgtaa gtccaggact
     2821 cattgatctt catgtacacc tcagggagcc tggttttgaa cacaaggaaa ccatcgaagc
     2881 tggtaccaaa gcagctgctg ctggcggttt tacacgcatc tatgcaatgc caaacaccga
     2941 accgccatgt gacaacgagg catctgcaat ctacataaga caacaggctg ataggctggg
     3001 attgataagg gtagagcctg tcggctcaat atcacagggc cgcaagggcg agttcctggc
     3061 cgagatgggc ggacttgcaa gggccggcgt caagatggtc accgatgatg gcaatccggt
     3121 ttcgtcagct tcgttgatgt tgcttgcact aaaatactcc aaagctttcg ggatgctcgt
     3181 ggccgaccat tgcgaagaca agtcgatatc ggccaatgga atctgtaatg aagggtattt
     3241 ctcaacactc tacggcctcc aggcatggcc aaaatcggcc gaggagacca ttgtttccag
     3301 ggacattatt ctcgctggcg aaacagacag taggatccac atcatgcaca tctccaccaa
     3361 attctccgcc cagttgatcg cgcaggcaaa atcaatgggc ataaaggcaa cctgtgaagt
     3421 cacaccgcat catctcacac tcacagacga gctccttggc gactacaaca caaactggaa
     3481 agtcaatcct ccgctaaggg gcaaggaaga catagaggcc ctgagaaaag cactcgccga
     3541 cggaataata gactgtatag caactgatca tgcgccacat gccagggaag acaaggaagt
     3601 tgaaataaat atagctccac ctggtatatc cggcctcgaa actgcagttg gtcagctttt
     3661 caccgaactg gtaagcaagg gtgtaatctc ccctgaaaag ctcctttcaa ggatgactgt
     3721 tggtcctgca aatgtagtcg gactggaaat cccggaaata aaagcaggaa aaccggc
//
LOCUS       DAOSRI010000168         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_21970_length_3759_cov_3.322084, whole genome shotgun sequence.
ACCESSION   DAOSRI010000168 DAOSRI010000000
VERSION     DAOSRI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21970_length_3759_cov_3.322084"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK717_05675"
     CDS             complement(<1..257)
                     /locus_tag="PK717_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOR47187.1"
                     /translation="MEKIALLALGGSIYSQDIKLCGNCSFSCAADSGLDGLLSVGITP
                     NLVIGDMDSVNPKNLSKIKNLGIEIISYPEQKNATDGELAI"
     gene            complement(245..1774)
                     /locus_tag="PK717_05680"
     CDS             complement(245..1774)
                     /locus_tag="PK717_05680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR47188.1"
                     /translation="MSILVKASHIWGKEVTDNSILVENGKIKQIGFFDDLSKNFSGEV
                     HDYKKGTIIPGFCDAHLHMAWLGQTLTLCDLRGCKSSVEFLNKIRAYAKKLAPGEFLR
                     GFGWDDRAWSDHEKPTRWLIDDATCGHKCSLTKVDGHSFLINSEFMKECEVTKETPDL
                     SGGRIGKNQDGEPDGMFYDSAFDEYARKKIPSPTTDQLEGFLLKAAEHLLSYGITSCR
                     SFGTLDDFVTLAHMDRKGILKIRTCACIPENALAWAVDLSAKTGTGSKMFWTGQLKLF
                     ADGSLGSKTALVSEPYSDGTKGLEVTSVPQIKEKVTMAHQIGLGVAIHAIGDEAVKNV
                     LRVFGESSGQDTVEHFQCAKPQSIALAAKLNIPVVVNPSHMPLDAEAIKAEWPRLAQY
                     SYPLNSLIKAGVTIGFGSDAPVVNPNPLTALACATTRGRTTDDQVNSQEAISFSQAIR
                     IATKESSSIIGGPKRGVLEEDFAADFAILSEDIRGKDPWDVEKVPFLATYVAGEIVWK
                     K"
     gene            complement(1771..2958)
                     /locus_tag="PK717_05685"
     CDS             complement(1771..2958)
                     /locus_tag="PK717_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOR47189.1"
                     /translation="MFRDITSNLGLKITALLIAVAIWLYVSQSQGPEVTKSFIVPVQT
                     RNLPENIANTTTLPNVTVTLRGSKILLEKIREENVNAYVDFLDKKQGKDQVFVKVDFP
                     QTVRLVDIDPNVVSVDLQKLQEKELPIVVTYYGQAPAGITLGEAQTDPKRLIIYGPSQ
                     GLSNIKEAHVKLNRALVKEGPNRFSMSYDLVDNNGKPIKKSALEEMKIRPKFDTISVE
                     IKSSSKQDIKTVGVIANTTGTLPTDRVITEIDWAPKSITISGPYATISKIESIYTEPY
                     NLNGILSTTSVMVPLSIPPDVLADVSEVRITIKIEPLSRKKLNLYVDVKPTDKQYEII
                     DRIIEVTFQGPSSIIATLTTASATIDVSGLPQGEQNVKVQISGLPDGITVLQTPTVKV
                     KII"
     gene            complement(2948..>3759)
                     /gene="cdaA"
                     /locus_tag="PK717_05690"
     CDS             complement(2948..>3759)
                     /gene="cdaA"
                     /locus_tag="PK717_05690"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOR47190.1"
                     /translation="PVPETIFDILIIAIIIYYVLKIVQASKALQLFQGLAVFFLALII
                     SDNISRWLGLIALNWMIRSVLQVLPMTLPILLAILFQPELRKAIGTLGQKSVFSKAID
                     FMEAQEVNTLVNEICQAVGHMSDRKHGAIIAIERQVGLGDYENGATKIDAQANASLIE
                     TIFYPGSALHDGGIIIRGERIAYARCVFPLSQDPNLDPDIGTRHRAAVGLSEETDAII
                     VVVSETSGIISLATAGSLTRYLSRVELEGLLKYMIKSRGKVFANLRRPNVS"
BASE COUNT          888 a          823 c          853 g         1195 t
ORIGIN      
        1 tctatggcca attcgccatc tgtggcgttt ttttgttctg ggtaagaaat tatttctatc
       61 ccaagatttt ttatttttga aaggtttttg ggattgacgg aatccatgtc cccaatgaca
      121 agatttggag ttataccaac cgagaggaga ccatccagcc ctgaatcggc tgcgcatgag
      181 aacgaacagt tgccacaaag tttgatgtcc tggctataga tggaaccccc aagtgcaagc
      241 agggctattt tttccaaact atctctcctg caacgtatgt tgccaagaac gggacctttt
      301 cgacatccca ggggtcttta cctcttatat cttcagatag gattgcaaaa tcggccgcaa
      361 agtcttcttc cagaacgcct cttttggggc caccaatgat gctggaagat tcctttgtgg
      421 cgatgcggat tgcctgtgag aagcttatgg cctcttgcga attgacctga tcatcggttg
      481 ttcttcctct tgttgttgcg caggcaagtg ccgtaagggg atttgggtta acaactggtg
      541 catcactgcc aaaaccaatg gtgacgcctg cttttatgag tgaattaagt gggtatgaat
      601 attgtgcaag ccttggccac tcagctttga ttgcttcagc gtccagtggc atgtgcgatg
      661 ggttgaccac caccggaatg tttagctttg ccgctagagc aatgctttgc ggttttgcac
      721 attgaaaatg ctctaccgtg tcctggccac tgctttcgcc aaaaacacga agaacatttt
      781 ttactgcttc atcacctatt gcatggatgg cgacacccaa accaatctgg tgggccatcg
      841 tgaccttttc cttaatctgg ggaacgcttg tcacttctag cccttttgtg ccatctgaat
      901 atggttcgga caccaaagca gtttttgaac caagtgaacc atcggcaaac aattttagct
      961 ggcctgtcca gaacattttg gagccagtcc ccgtttttgc tgaaaggtcg actgcccagg
     1021 ccagagcatt ttccgggatg caggcacatg tccttatttt gagtattccc ttgcggtcca
     1081 tgtgggccag tgttacaaaa tcatcgagtg taccgaacga cctgcatgat gttatcccgt
     1141 atgataggag gtgctctgcc gcttttagta ggaaaccttc aagctgatcg gttgttggtg
     1201 aagggatttt ttttcttgcg tattcatcaa atgcgctgtc ataaaacatt ccatcaggtt
     1261 cgccgtcctg atttttaccg atccttccgc ctgaaagatc aggggtttct tttgttactt
     1321 cgcactcttt catgaattca gaatttatca aaaaagaatg tccatcaacc ttggtcaggg
     1381 agcatttgtg gccacaggtt gcatcgtcta ttaaccacct tgttggtttc tcatgatcag
     1441 accaggccct gtcgtcccag ccaaaccccc tcagaaattc tcctggggcc aatttttttg
     1501 cgtatgccct tattttattt aggaactcga cagatgattt acatcccctg aggtcacaga
     1561 gtgtcagggt ttgaccaagc cacgccatgt gaaggtgggc atcacaaaaa ccaggaataa
     1621 ttgtaccctt tttgtaatca tggacctcac cactgaagtt ttttgaaaga tcatcaaaga
     1681 agccaatctg ttttatcttg ccattttcta caagaattga attgtcggtg acttctttgc
     1741 cccagatgtg cgatgctttg acaagaatgc tcatattatt ttcactttta cagttggtgt
     1801 ctggaggact gttattccat ctggaaggcc actaatttga acttttacat tctgctcgcc
     1861 ctgtgggaga ccagacacat caattgtcgc tgatgcggtt gttagagtgg ctataattga
     1921 tgatggtccc tggaatgtga cctcgattat cctgtcgatt atttcatact gcttgtctgt
     1981 tggtttgacg tcaacgtaga ggttcaattt ttttcttgaa agtggctcaa tctttatggt
     2041 tatcctgacc tctgagacgt ctgccaagac atccggaggg attgaaagcg gtaccattac
     2101 gcttgtggtg ctcagtatgc cgtttagatt atatggctcg gtgtagattg attcaatttt
     2161 tgagattgta gcgtaagggc cagaaattgt tattgatttt ggtgcccagt ctatctctgt
     2221 tatgacccgg tccgttggca gtgtgcccgt ggtgtttgct attacgccaa cagttttgat
     2281 atcttgtttt gaagagcttt tgatttcaac gcttatggta tcaaattttg gccttatctt
     2341 catctcttcg agggcagatt ttttgattgg tttcccgtta ttatcaacca aatcataaga
     2401 catgctgaat ctgtttgggc cttcttttac aagtgccctg ttcagtttta catgcgcctc
     2461 ttttatgttg gagagccctt gggatggtcc atatataatt aatctttttg gatcagtttg
     2521 ggcctcgcca agtgttatgc cagcaggtgc ctgaccgtaa tatgttacga cgattggaag
     2581 ttctttttcc tggagttttt gaaggtccac agagacaaca tttggatcaa tatcaacaag
     2641 ccttactgtt tgcgggaagt ccactttgac aaaaacctgg tctttaccct gttttttgtc
     2701 caaaaaatcg acgtatgcat tgacgttctc ttccctgatt ttctcaagca atatttttga
     2761 acccctgagc gttacggtaa cattcggtaa agtagtggta ttggcaatat tttctggcaa
     2821 gtttcttgtc tgtactggta ctataaagct ttttgttacc tctggcccct ggctctgtga
     2881 aacataaagc cagatggcaa ctgctataag caaggcagtt atcttcaggc caagatttga
     2941 tgtgatatca cgaaacattt ggcctcctta agttggcgaa caccttgccc ctgctcttta
     3001 tcatgtattt taacagacct tcaagttcga ctctcgaaag gtatcttgta agcgaacctg
     3061 cagtggcaag tgatattatt ccggaggtct ccgagacaac cacaataatt gcatcagtct
     3121 cttcggaaag gccaacagcg gccctgtgcc tcgtcccgat gtcaggatcg agatttgggt
     3181 cctgggagag cgggaaaacg catcttgcgt aagctattct ttcgcccctg atgattattc
     3241 cgccatcatg gagtgcagaa cctgggtaaa atattgtttc tatgagtgac gcgtttgcct
     3301 gtgcgtcgat ttttgttgcc ccattctcat agtcaccaag acccacctgt ctctcgatgg
     3361 cgattattgc accatgtttc ctgtctgaca tgtggccaac tgcctgacag atttcattga
     3421 ctagagtatt gacttcttgg gcttccatga aatctattgc tttgctaaaa actgatttct
     3481 ggccaagtgt gcctatggct tttctgagtt ctggctggaa cagaattgca agaaggattg
     3541 gcagggtcat tggaagaact tggagaactg acctgatcat ccaatttagg gcgatcaaac
     3601 cgagccatct tgatatgttg tcgctgataa tgagagccag gaagaaaact gcgagaccct
     3661 ggaaaagttg gagtgctttt gatgcttgaa cgatttttag tacataatat atgatgattg
     3721 ctattattag aatatcgaag atggtttcag gaactggta
//
LOCUS       DAOSRI010000169         3746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22058_length_3746_cov_5.076131, whole genome shotgun sequence.
ACCESSION   DAOSRI010000169 DAOSRI010000000
VERSION     DAOSRI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22058_length_3746_cov_5.076131"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /gene="cgtA"
                     /locus_tag="PK717_05695"
     CDS             <1..472
                     /gene="cgtA"
                     /locus_tag="PK717_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOR47191.1"
                     /translation="VKLYSEETFLKPAFVVGNKIDLMEGKPDPLEDFISKRNIPYLKI
                     SAKERIGTENLIDTLWQKVKDVPLPQAKKSEEVIIVSKVQTDTKIERSDGGEFLVKNS
                     YLERIVAKTDLESLDGLAFVYKQMNRFGIEKKLKQMGIKEGDKVTIAGKRFTFE"
     gene            483..1082
                     /gene="nadD"
                     /locus_tag="PK717_05700"
     CDS             483..1082
                     /gene="nadD"
                     /locus_tag="PK717_05700"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOR47192.1"
                     /translation="MGGTFNPPHNAHLLLGVFAREQYQLDEIVIVPGSTFSPNKNRPQ
                     IDGSHRLNMARIACEDNPSFKVSDREIGPETIYTVETLSALKSGDPENEFFFIAGSDL
                     MATFKNWREPEKIARLTNFIVAPRFGASFEEARSVLMECGATDKTVFMLDFPRIDISS
                     TLVRERLEAGLDCRYLVPNEVLMYIEKNKLYKEAKAGTP"
     gene            1084..1392
                     /gene="rsfS"
                     /locus_tag="PK717_05705"
     CDS             1084..1392
                     /gene="rsfS"
                     /locus_tag="PK717_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR47193.1"
                     /translation="MAKKVVEILDELKAENIVLLDIKGMTPMCDYFIVATIISPLQSQ
                     AIVDRLEQFGKKQKMPRNLEGGASSKWILVDLGDLIVHLFGKEERIYYNLEELWKRKP
                     "
     gene            1374..1922
                     /locus_tag="PK717_05710"
     CDS             1374..1922
                     /locus_tag="PK717_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47194.1"
                     /translation="MEKKTLILLVCLAIAFTAVSCGGQSKQGQDGSGSVYEVMGKEFQ
                     VRGCTFNVTKLTKSVDIRAIKKDGTTDSPLWPEQGFFYLLEMEIKPGETPTFQPKFTR
                     YEIVDVDGNRYYETKNEMAIQVAEKNLGVKSLTKIKDDITKPVKCFLLMDCQQNMKGV
                     TLEIISTASDPEQRLAVVDLKE"
     gene            1919..2485
                     /locus_tag="PK717_05715"
     CDS             1919..2485
                     /locus_tag="PK717_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47195.1"
                     /translation="MKKYLLLFLGGFIVLAGCGAKGLPSVAPDVERINTPRSTDFVYT
                     GPQTDFQLLPGLQAKIVSAKDHYSLVNPDKQSPFPEIYSPKGRFLIATLEAKKIGEIT
                     QKTLPPMESIAVVGWDNPLCNDESINRQIEMITGLKRLEPAIVTSEPKKYFLAFVVEG
                     ASYGWVINAYRDNPAGDKELYLTVDTGI"
     gene            2762..3487
                     /locus_tag="PK717_05720"
     CDS             2762..3487
                     /locus_tag="PK717_05720"
                     /inference="COORDINATES: protein motif:HMM:NF027286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HOR47196.1"
                     /translation="MQANDAIMSILTFYGVAIALLTFFVTVISLIFIRKSIDKSNENN
                     ANQEKNMRLESYTKIIDMMENAWNEDNSIELINSFKELDEPSTIKNYESLDIVDDKDK
                     NKITQKRIILLIRSIDKLGLLVKHGVVPVNFVSDFYSSRIVSTWRMLWPVIEKLRKEK
                     KHPGLYTKVEILTYIATTHRAKWHDETCIGKNMKDDQIIEYWKGNTGKYFGKNPRKWG
                     EPGFEGENWGKTMWVEKGMGIYD"
BASE COUNT         1178 a          713 c          840 g         1015 t
ORIGIN      
        1 agtcaaactt tattcagaag agactttttt aaaaccagct tttgttgttg gaaacaagat
       61 cgatcttatg gaaggaaaac cagatcccct ggaagatttt atttcaaaga gaaacattcc
      121 atacctgaaa atctctgcaa aagagaggat tggcacagaa aatctgattg atacactctg
      181 gcagaaagtc aaagacgttc ctctaccaca ggccaaaaaa tctgaagaag tgataattgt
      241 ttcgaaagtg cagaccgata caaaaatcga gcgcagtgat ggtggggaat tcctggtcaa
      301 aaattcatac ctggagagaa ttgtcgcaaa aaccgacctt gaaagcctcg atggtctagc
      361 atttgtttat aaacaaatga atcgctttgg cattgagaaa aaattgaagc agatgggcat
      421 aaaagaaggc gacaaggtga ccatcgccgg aaagcgcttc acttttgagt aggattggga
      481 tattgggtgg aaccttcaat ccaccccaca acgcgcacct cttgcttggc gtttttgcca
      541 gggagcaata ccagcttgat gagattgtca ttgtccctgg ttccactttt tctccaaaca
      601 aaaacaggcc acagatagat ggttcccata gattaaatat ggcccgcatc gcctgtgaag
      661 acaacccatc ttttaaggta agcgatagag agattggccc agagaccatt tatacagtag
      721 agaccctgtc ggccttgaaa tcaggggatc ctgaaaatga gttctttttc attgctggtt
      781 cagatttgat ggccactttt aaaaactggc gggagccaga aaagattgcc agacttacca
      841 attttattgt tgccccaaga tttggggcaa gctttgaaga ggccagatct gttttgatgg
      901 agtgcggtgc gactgacaaa acagtattca tgcttgattt cccaaggatt gatatttcct
      961 cgaccctcgt cagggaaagg ctagaagcag gtttagactg cagatatcta gttccgaacg
     1021 aggttttgat gtatattgaa aaaaataaac tctacaagga ggccaaagct ggaacccctt
     1081 gaattggcaa aaaaagtcgt agaaattctc gatgagctga aggctgagaa cattgtattg
     1141 ctggatatca aaggaatgac gccaatgtgc gattacttta tcgtcgcaac aatcatttct
     1201 ccgctccaga gccaggccat cgttgacagg ctggagcagt ttggcaaaaa gcaaaaaatg
     1261 ccaagaaatc ttgagggcgg ggcttcttca aaatggatac ttgtcgatct cggtgactta
     1321 atagtccacc tgttcggcaa ggaggagagg atttattata acctggagga attatggaaa
     1381 agaaaacctt gatattgctt gtttgtctgg caattgcctt cacagcggtt tcatgtggtg
     1441 gccaatccaa acagggtcag gatggcagtg gctcagtata cgaagtaatg ggaaaagagt
     1501 ttcaggtcag aggctgcacc ttcaatgtca caaagctgac aaaatctgtt gacatcaggg
     1561 caatcaaaaa agatggaaca acagattccc cgctgtggcc agagcaggga tttttctacc
     1621 tcctggaaat ggaaatcaag ccaggtgaaa cgccaacttt ccagccaaag tttaccaggt
     1681 atgagatcgt cgatgttgat ggcaacaggt attacgaaac caaaaacgaa atggccatcc
     1741 aggtcgctga aaaaaacctt ggtgtaaaat ctttgacaaa gatcaaagat gacataacaa
     1801 aaccagtaaa atgtttcctt ctaatggatt gccagcagaa catgaagggt gtcacactcg
     1861 aaatcatctc tacagcttct gatccggagc agcgcctcgc agttgttgac ctaaaagaat
     1921 gaaaaagtat ttattgctgt ttcttggagg gtttattgtt cttgcgggtt gcggggcaaa
     1981 aggcctccct tctgttgctc ccgatgttga aaggataaac actcccagat cgacagattt
     2041 tgtttatact ggcccgcaaa ccgatttcca gctattgccg ggcctccagg ccaagattgt
     2101 ctctgcaaaa gaccactaca gccttgtcaa tccggacaag caaagtcctt ttcctgagat
     2161 ttattcccca aagggcagat ttcttatagc gacccttgaa gcaaaaaaaa ttggcgagat
     2221 aacacaaaaa acccttccgc caatggaatc gattgctgtt gtcgggtggg acaatccatt
     2281 gtgcaatgat gagagcataa acaggcagat tgaaatgata accggcttga aacgcctgga
     2341 gccggcaatt gtcacatccg aacccaaaaa atattttctt gcctttgtgg ttgagggtgc
     2401 ctcttacggc tgggtcataa atgcctaccg cgataatccg gctggcgaca aagagcttta
     2461 tctgactgtt gacactggga tataataccc tctagctttt aaaagatggg gaggtgttcc
     2521 atgtacacca caggtatata cttcgaaagt tttttatgtt tgttgggtgc gttgctaatt
     2581 attgggggga tggttttgtc tttctggtgt tttgataaaa tcaatggaaa caatccaggt
     2641 cgttcatttt ttttctatat cacttacttt gttcttttca ttttttgttt gttcttattg
     2701 tatgtacctt tatgcaattt cttacattga aaatattgaa tctagaaaga ggtaggatcc
     2761 aatgcaagca aatgatgcaa ttatgtcgat actaacattt tatggagtgg caatagccct
     2821 cctgaccttt tttgtaacag tcatatcgct gatttttatt cgaaaatcaa tagataaatc
     2881 aaatgaaaac aatgcgaatc aagaaaaaaa catgaggctg gagtcttaca ccaaaataat
     2941 tgatatgatg gaaaacgcct ggaatgaaga caattctatt gaattgatca attcatttaa
     3001 agagcttgat gaaccatcaa ccataaagaa ctatgaaagc ttggatattg ttgatgataa
     3061 agataagaac aagatcacac aaaaaagaat aatactgttg attagaagca ttgacaaact
     3121 tggtttgctt gtgaagcatg gcgttgtgcc ggtaaatttt gttagcgatt tttattcaag
     3181 cagaattgta agtacctgga ggatgttgtg gcctgtcatt gaaaaattga gaaaagaaaa
     3241 aaaacatcct ggattgtata caaaagtgga aattttgacc tatattgcaa caactcatag
     3301 agcaaagtgg cacgatgaga cctgcatagg gaaaaatatg aaggatgacc agatcattga
     3361 atactggaaa ggtaacactg gaaagtattt tgggaaaaat cccagaaaat ggggtgaacc
     3421 tggttttgaa ggtgagaact ggggaaaaac aatgtgggtt gaaaagggaa tggggattta
     3481 cgactagact aacaaaattt tacgtcaatc acctgtcagc ttgctactct tcagatgaat
     3541 atcctgtcta tttttatggc atccggcccc attcaatcaa attatatgaa ccacccagtc
     3601 cgccgatcaa ttttaaataa agtctgcggt tccagctttc tcttagacct gtcctgaaac
     3661 ttggatggga tttgggaaga ggcctcttct atgtatgaga aaacatccaa ttaaaaagcc
     3721 gccccaaaag ggcggctttt ttattg
//
LOCUS       DAOSRI010000170         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22142_length_3736_cov_2.945939, whole genome shotgun sequence.
ACCESSION   DAOSRI010000170 DAOSRI010000000
VERSION     DAOSRI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22142_length_3736_cov_2.945939"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PK717_05725"
     CDS             complement(<1..1089)
                     /locus_tag="PK717_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOR47197.1"
                     /translation="MSEEEKKETLGSKLFYKTKNVWETTENHAEIMNFAKPYKTFLDQ
                     CKTEREAVAWAAKKLEDAGFKTPGNGDKIFMVNRGKAVIAARLGKKPIKEGFNIVVSH
                     VDAPRLDLKQVPFYEDSGHAMLDTHYYGGIKKYQWANIPLAMHGRIIKADGSKIDLVW
                     GEDENEGSLLIPDILIHLAQKQMEGSAAKALTGEQMNAIAGTIPIPGKEYEKESDKVK
                     LNVLKILNDKYGIMEADFVSAEIELVPSENCREIGIDNSCIIGYGHDDRICSYSALQA
                     LLDMKEQPERTAIAYLVDKEEIGSEGTTGAITKFLEYFIEKIAGTCDTTEIMQNSFCM
                     SGDVNAGYDVNFKENFEAKNASFLGKGIT"
     gene            1209..2156
                     /gene="mraY"
                     /locus_tag="PK717_05730"
     CDS             1209..2156
                     /gene="mraY"
                     /locus_tag="PK717_05730"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOR47198.1"
                     /translation="MESLIFSFIFSLVFSWPVIWLMRKLKAGQQVREEGPKTHYAKAG
                     TPTMGGFIFILATLLAAIISPVKTKDVFWGVYALLAYGLVGFIDDYIKVFRHSPYGLS
                     GRWTVILQVIFALPFLIFNWGTLPSAFGQSAPNWLNFAFYLFIMVGGVNAANLADGLD
                     GLAAGASSITLLCLAPIIWATQPTATVVPLALAGALLGFLFVNLKPAKIWMGNFGSNA
                     IGGVLVVSAIYSGHIWLFAVGAGLFIIEALSSMIQVGYFKYTKYISPLKKGVRIFKMS
                     PIHHHFELSGWKEIKVVVVFWVASAVFGLLMLGLFYLLY"
     gene            complement(2164..2370)
                     /locus_tag="PK717_05735"
     CDS             complement(2164..2370)
                     /locus_tag="PK717_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOR47199.1"
                     /translation="MTVDIRSKSIMCSGCAQTITKALMGVIGVKSVSVDVPKKTVTVD
                     FDERTTSELVLRTVMIGAGYPPDA"
     gene            complement(2367..2915)
                     /locus_tag="PK717_05740"
     CDS             complement(2367..2915)
                     /locus_tag="PK717_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOR47200.1"
                     /translation="MKNSWFSFLVHLRSLVTVSILTALAIASKFATFFIGATKFDPAG
                     PAVIMIAGMMYGPIAGAIVGGLADFLGFLVYNPTGFAWNPVVTVGNICLGFFAGLFYW
                     IFKYFSKPKQAAALPIIISTFAGRLIGFFVTTWGLIATYKMDFYGTLMARIPSEPFLM
                     IWYIVVTLAIMLWVEKNKWSEE"
     gene            complement(3120..>3736)
                     /locus_tag="PK717_05745"
     CDS             complement(3120..>3736)
                     /locus_tag="PK717_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47201.1"
                     /translation="PLGVDYKPVSINLFHSGRLEKIAALALCLNSEFFFLISPKSGYA
                     GNLLREGMFMPDKNPGVELGFTMLFGYSPAMEEIIALILKDQRDIAGGVSYCNLKNGR
                     FVRETFKAIILVSGNPFFGEDEFLDRLGEVLEKNAPFGGNMAFFAETFGYRRFFIPYE
                     TDNPVRLGISRLLFEMEFEETRRLVGPSPEISFPVFLTIGLVSR"
BASE COUNT          877 a          901 c          850 g         1108 t
ORIGIN      
        1 ggttatgccc ttaccaagga aagaggcatt cttcgcttcg aaattttcct tgaagttgac
       61 atcataccct gcatttacat cacctgacat gcagaacgaa ttctgcatga tttctgttgt
      121 atcgcatgta ccggcaatct tctcgataaa gtattcaagg aattttgtga ttgcaccggt
      181 tgtaccttcg gaaccgatct cttccttgtc gacgaggtat gctattgcgg tacgctctgg
      241 ctgttccttc atatccagaa gggcctggag tgcggaatag gaacatatcc tgtcatcatg
      301 gccgtagcca atgatgcagg agttatctat gccaatctcc ctgcaatttt ctgatgggac
      361 aagctcgatc tctgccgaga caaaatctgc ctccatgatg ccatatttgt cgttgaggat
      421 tttgagaaca ttcaatttta ccttgtctga ctctttctca tattccttgc ctggtatcgg
      481 gatcgttccg gcgatagcgt tcatctgttc gcctgtgagt gcctttgcgg ccgacccttc
      541 catctgcttc tgtgcaaggt ggatgaggat atctggaatc aggagtgatc cctcattctc
      601 gtcttcaccc cagacaagat caatctttga gccatcagcc ttgattatcc tgccgtgcat
      661 ggcaagcggt atgttggccc actggtattt ttttattccg ccatagtagt gggtatcgag
      721 cattgcgtgc ccggaatctt cgtagaatgg cacttgcttg agatccagtc ttggtgcatc
      781 aacgtgtgaa accacaatgt tgaagccttc cttgatcggc tttttgccaa gccttgcagc
      841 gatgactgcc ttgcccctat tgaccatgaa gattttgtct ccattgccag gtgttttgaa
      901 accagcgtcc tcaagctttt ttgcagccca agccacagct tccctttcag ttttgcactg
      961 gtcgaggaat gttttgtatg gttttgcaaa attcatgatt tcagcgtggt tctctgtggt
     1021 ttcccaaacg tttttggttt tatagaacag cttggaaccc agcgtttctt ttttttcttc
     1081 ttcgctcatg ttgcctccta taatataaac acaaatctaa tctaaaacta tttgggtaaa
     1141 aacaaaactt accctcactt tacacgaaac actcggaagt tataatgcct tgtatttgtg
     1201 aggtgaaaat ggaatctttg attttctcgt ttatattttc gcttgttttt tcatggccag
     1261 tcatttggct tatgcgcaaa ttaaaagctg gtcagcaggt tagggaagaa ggcccaaaaa
     1321 cacattatgc aaaagctggg actccaacca tgggtggttt tattttcatt ttggcaacat
     1381 tgcttgctgc catcatttca ccagtaaaga caaaagatgt tttttgggga gtctatgccc
     1441 tgcttgctta tgggctggtg ggttttatag acgattatat aaaagttttc cgtcactcgc
     1501 catatggtct ttcaggtaga tggactgtaa tcctccaggt aatttttgct ttgccgttcc
     1561 tcatttttaa ttggggcacc ctgccaagtg cctttgggca atcagcccca aactggctca
     1621 atttcgcatt ctatctgttt atcatggttg gcggggtcaa tgcagccaat cttgcagacg
     1681 gtcttgatgg tctcgcagcg ggtgcttctt ccatcacttt gttgtgcctc gcgcccatca
     1741 tctgggcgac acagcctaca gcgacagttg tcccgcttgc tcttgcaggt gcccttctgg
     1801 gttttctctt tgtcaatctc aagcctgcaa agatatggat ggggaatttt ggctcaaatg
     1861 ccattggggg agtgcttgtt gtttctgcaa tctattcagg acatatctgg cttttcgctg
     1921 ttggtgcagg cctcttcatc atagaagcgc tttcctcgat gattcaggtg ggttatttca
     1981 aatacacaaa atacatatct ccgctgaaaa agggtgtcag aatttttaag atgagtccaa
     2041 tccaccacca ctttgaactt tccggctgga aagagatcaa agtggtggtg gtgttctggg
     2101 tggcatcagc agtttttggc ttactgatgc ttggcctctt ctacttgctt tactaggcct
     2161 ggatcaggcg tctggcgggt agccagctcc tatcattaca gtcctgagca caagctcgct
     2221 ggttgttctc tcgtcaaagt ccacggtgac tgtttttttg ggcacatcaa ccgataccga
     2281 cttgacccct atgacaccca tcagggcctt ggtgatggtt tgcgcacagc cactgcacat
     2341 tatactttta cttctgatgt ctaccgtcat tcttcactcc atttgttttt ttctacccag
     2401 agcatgattg ccagggtgac cacgatatac cagatcatta gaaatggttc tgagggaatt
     2461 cttgccatga gggttccgta gaaatccatt ttgtatgtgg caataagccc ccatgttgtg
     2521 acaaaaaatc ctatcaatct tccagcaaag gtagatatta ttattggtag tgccgcggcc
     2581 tgttttggct tggaaaaata tttaaaaatc caataaaaaa ggccggcaaa gaaaccaaga
     2641 cagatatttc caactgtcac taccgggttc catgcgaacc ccgtagggtt gtatacaagg
     2701 aaaccgagaa aatcagctaa acctccaaca attgcgccag cgattggccc atacatcatg
     2761 ccagcgatca ttattactgc aggaccagct ggatcgaatt ttgttgctcc aatgaagaag
     2821 gttgcaaact ttgaggcgat tgcaagggct gtaagaattg atacagtgac aagacttctt
     2881 aggtgaacca ggaaactaaa ccatgagttt ttcatattct ttttttactt ctttctcgtc
     2941 aaggaaaact gccttctcct gcagaaaatc tttttcctta agaatttcgt cctccatcga
     3001 gtccccagtc tccttttcat aacagcaaga gcggcccgtg ccccccggtt gcggggccag
     3061 ggtcgaacaa gagtactctt ccatctcctt ttgcgcagac agatattata ccaattttat
     3121 catcgcgaga caagcccaat ggtgagaaaa accggaaagg agatctcggg cgaagggcct
     3181 accagccgcc tggtctcttc aaactccatt tcgaagagca gccggctgat ccccagtctt
     3241 accgggttgt cggtttcgta gggtatgaag aagcgcctgt agccgaaggt ctcagcgaag
     3301 aatgccatgt ttccgccgaa gggggcgttc ttttcaagaa cctcccccag cctgtcaagg
     3361 aactcgtcct cgccgaaaaa ggggttgccg cttacgagaa ttatggcttt gaaggtctcc
     3421 ctgacaaagc gcccgttctt caggttgcag taagaaacgc cccccgcaat atcccgctgg
     3481 tccttcagaa taagggcaat gatctcctcc atggcggggg agtagccgaa gagcattgta
     3541 aagccgagct ctacgccggg gtttttatcg ggcatgaaca ttccctctct gagcagattg
     3601 cccgcatacc cgctctttgg gcttataaga aaaaagaact cgctgttcag acagagggcc
     3661 agggccgcaa ttttttccag gcggccgctg tgaaacaggt tgatcgacac cggcttatag
     3721 tccactccca ggggaa
//
LOCUS       DAOSRI010000171         3721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22256_length_3721_cov_3.526732, whole genome shotgun sequence.
ACCESSION   DAOSRI010000171 DAOSRI010000000
VERSION     DAOSRI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22256_length_3721_cov_3.526732"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK717_05750"
     CDS             <1..488
                     /locus_tag="PK717_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47202.1"
                     /translation="MEWKGTMNGFNETESKYGEKNVSWTNKYPNGSYSKEVKFYEEFH
                     DEDSVMWFTPTLGYEKDKQRLFTYFANRTAEPTQGMIWMGGTTKLEVRGKKYNAWAAG
                     IRCNEIVERVWYDMETNRLIRYEQDMGFLAYPDSNPKKPAGWEPPKTTFTLVLDSWET
                     K"
     gene            485..961
                     /locus_tag="PK717_05755"
     CDS             485..961
                     /locus_tag="PK717_05755"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOR47203.1"
                     /translation="MRQTLKTSWGPMVAVFRHGKLNGLGFGKGDCDAFEDCAKNLEVQ
                     LEEYFTLKRKSFDIQIDPDGTDFQKAIWNACREIPYGSVLAYSGLAAKIGRPNSQRAC
                     GNALGQNPILIVVPCHRVVAKHGIGGFGGKPGDLTMKIRLLELEKPGISSNLHQIS"
     gene            complement(903..1586)
                     /gene="fsa"
                     /locus_tag="PK717_05760"
     CDS             complement(903..1586)
                     /gene="fsa"
                     /locus_tag="PK717_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOR47204.1"
                     /translation="MQIFLDTASVEEIKRALSFGVVSGVTTNPTLMSRESGASFKDTI
                     LKIAGMVPGPVSAEVTVHDAKGMINQAMEITGWSDSDDFRDRITIKIPMTSEGTSAIK
                     ELSSRGISTNCTLVFTPVQAMLACEAGATFISPFVGRIDDSGLDGMSVLKDIVSMVRT
                     NGYVSQIIAASIRHPAHVYGAAMLGCDIATIPFTVLEQCFKNPLTDIGIRKFDEDWKK
                     FQASQAPKA"
     gene            1664..>3721
                     /locus_tag="PK717_05765"
     CDS             1664..>3721
                     /locus_tag="PK717_05765"
                     /inference="COORDINATES: protein motif:HMM:NF040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47205.1"
                     /translation="MKKFTIALTVIIAINFVPQGSFGASNNASWSMAGCTPDRRHASS
                     AKIQPPLDYSWDTGQEGSIWAVPPLASGSACYLGMSKPPEKGIYQTSIQKRVLSSGSI
                     AWEYKEAWWPWALYKGDLICQGYSNEKQYSWVRRINGESRATVWEIEWLRYTIGAIID
                     GDIIYSLSYSDVKDDNDKDQRIFFFQAHSAENGKLIWKKSYEYNITMVPWFCVQGDYL
                     YGSIFKTIYKLDKNTGAEIWKIQNNERIGRGSYIVGTEKGIFVNTVYDRLNYISLEDG
                     KTIWSKRLSDYESSDPDNCGMPSGTPSILGNRVYIVSRGSKDKNQPKVVYCLDLETGK
                     TIWQVALQGTSTIEGSDIGHTTTVANGVLYTMTEDNSGLNTKLRAFDPENGMLLWQDS
                     IPGVPNPSEMTVVSGYLVIGMEVHRLEHKETDPVKSDFSYRCYTNIGVKPPVLYVEEN
                     ELNFGYTNEKKAITKKIAIQNDGSGELSGVLTSDDDWLLVSPTQFSGNSTEITVIADI
                     AKLKPGIYKSSINIKSNGGKKTIPVLVAFGGGQEPQTKSERVVIRCSETQAFKKIVKL
                     SGYNHGDVIVKAPWLIVNPIVFDGEDVSIAFIINKNLLSKTNNTGMAQIKSNAFKIDL
                     SFEVVGFSDPMTIIMWIGKDETLVNGKKVKLEAPPMITSGVTVVPIRFVAESFGVELK
                     YEAS"
BASE COUNT         1187 a          806 c          852 g          876 t
ORIGIN      
        1 agatggaatg gaaaggcaca atgaatggat tcaacgagac cgaatcaaaa tatggagaga
       61 aaaatgtcag ctggacaaac aaatatccaa acggttcata tagcaaagaa gtaaaattct
      121 atgaagaatt ccacgatgaa gattctgtaa tgtggtttac gccaacgctt ggctatgaaa
      181 aagacaaaca aaggctgttc acatactttg ccaaccgaac agcagaacca acacagggca
      241 tgatatggat gggtggaaca acaaagctgg aagtcagggg caagaaatac aatgcctggg
      301 ccgctggaat aaggtgcaac gagatcgttg aaagggtatg gtatgatatg gaaaccaaca
      361 gattaataag atatgagcaa gacatgggtt tcctggcata cccagattca aaccccaaga
      421 aaccggctgg ctgggaaccg ccaaagacga ccttcaccct cgttctagat agctgggaga
      481 caaaatgaga caaaccctca aaacaagctg gggtccaatg gtggcagtgt ttcgccatgg
      541 aaagctcaac gggcttggtt ttggaaaggg tgactgtgac gcattcgagg attgcgcaaa
      601 aaacctcgag gttcagcttg aagaatattt tactctcaaa agaaaatcat tcgacatcca
      661 gatagatcct gatggcaccg atttccagaa agcaatatgg aacgcatgcc gcgagatacc
      721 gtatggcagt gttcttgcct attccgggct cgcggcaaaa attggcaggc caaactcaca
      781 gagggcctgc ggcaatgcgc ttggccaaaa cccaatcctg attgttgttc cttgccacag
      841 ggtagtggca aaacatggga tcggcgggtt tggggggaaa ccaggcgatc ttacaatgaa
      901 aatcaggctt ttggagcttg agaagcctgg aatttcttcc aatcttcatc aaatttcctg
      961 attccaatat cagttagtgg gtttttgaag cactgctcca gcactgtaaa cgggatggtt
     1021 gcaatgtcgc aaccaagcat tgccgctccg tagacatgcg caggatgacg gattgatgct
     1081 gcaattatct gagaaacgta gccattcgtc ctgaccatcg agacaatatc ctttagaaca
     1141 gacattccat caagcccaga atcatctatc ctgccaacaa acggcgaaat aaacgttgct
     1201 ccggcctcac aggcaagcat cgcctgaact ggggtgaaaa ccagagtgca attcgtagag
     1261 attccccttg agctgagctc ttttattgcc gatgttcctt cagaagtcat cggtattttg
     1321 atggttatcc tgtccctgaa atcatcactg tcggaccacc cggtaatctc cattgcctga
     1381 ttgatcatgc cctttgcatc atgaacagtg acttctgctg aaactggacc cggcaccatc
     1441 cctgcaattt taagaatggt gtctttgaag ctggcacctg attccctgct catcagagtt
     1501 ggatttgttg tcacacctga aacaacacca aaagaaaggg ccctctttat ctcttctacc
     1561 gaagctgtat caagaaatat ctgcatgtta aacctccttt gacattatat ctaagttgac
     1621 tggcatttga agcaaagata gaattccctc aaaaggaggc gttatgaaaa agtttactat
     1681 cgccctcaca gtaatcatcg ccatcaattt cgtaccacag gggagttttg gggcttcaaa
     1741 caatgcttca tggtcaatgg ctggttgcac accagacaga agacacgcca gctcagcaaa
     1801 aatccagcca ccacttgatt attcatggga cacggggcaa gaaggctcaa tatgggcagt
     1861 accaccactt gcatcaggat ctgcatgcta cctcggaatg tcgaagccac cggaaaaggg
     1921 catttaccag acgagcattc agaaaagagt gctctcctct ggctcaatag catgggaata
     1981 caaggaagca tggtggccat gggcgcttta caaaggtgat ctgatttgcc agggttactc
     2041 caatgaaaaa cagtacagct gggtacgcag gataaatggc gaatcgaggg ccacggtttg
     2101 ggaaatagaa tggctaaggt acactatagg cgctattatc gatggagaca taatttattc
     2161 actttcatac tcagatgtga aagacgacaa cgataaggac cagaggatat ttttcttcca
     2221 ggcccattct gcggaaaacg gcaagctgat atggaagaaa tcctatgaat acaacatcac
     2281 tatggttccc tggttctgtg tccagggtga ttatctttat ggttcaatct tcaaaacaat
     2341 atacaaactc gacaaaaaca ccggcgccga gatatggaaa atccaaaaca atgaaagaat
     2401 agggagaggt tcatacatag tcggaaccga aaaaggtatc tttgtcaata ccgtttatga
     2461 cagactcaat tacatttcgc ttgaagatgg aaaaacaatc tggagcaaga ggttgtctga
     2521 ctatgaatca agcgaccccg acaattgcgg gatgccatca ggaacaccat caattcttgg
     2581 caacagggtc tatatagtat cgagggggag caaggacaaa aaccagccga aagtggtcta
     2641 ttgcctcgat cttgaaacag gcaagacaat ctggcaagta gcactacaag gtacatccac
     2701 aatcgaaggt tctgacatag ggcatacaac aactgtggca aacggtgttc tttacaccat
     2761 gaccgaagac aattctggac taaacacaaa acttagggct ttcgatccag aaaatggcat
     2821 gctactctgg caagatagta ttccaggtgt cccaaatcct tcagaaatga cggttgttag
     2881 tggttacctt gtgataggca tggaagtgca ccgtctggaa cataaagaaa ctgatcctgt
     2941 taaaagtgat ttttcctatc gttgctacac aaatataggt gtaaaaccac ctgttcttta
     3001 tgtggaagaa aatgagctca attttggcta cacaaacgag aaaaaggcca ttacgaaaaa
     3061 gatcgcgatc caaaatgatg gatcaggcga gctgtctggg gtattgactt ccgatgacga
     3121 ctggctcctt gtcagcccaa cacaattttc tggcaacagc acagagataa ccgtcatagc
     3181 agacatagcc aaattaaagc caggaatcta caaatcatcc atcaacataa aatcaaacgg
     3241 agggaaaaag accattcctg ttctggtggc ctttggtggc ggacaggaac ctcagacaaa
     3301 aagtgaaagg gttgtcatcc gttgcagcga aacacaagct ttcaaaaaga tagtaaagct
     3361 aagtggttac aatcatggtg atgtgatagt taaagcacca tggctcattg ttaatccaat
     3421 cgtttttgat ggcgaagatg tatcaatagc tttcataatc aataaaaacc tcctaagcaa
     3481 gacaaacaac actggtatgg cacaaataaa atccaacgca ttcaaaatcg atctttcctt
     3541 tgaggtcgtc ggattttctg atccaatgac aataatcatg tggattggaa aagatgaaac
     3601 actcgtgaat ggcaaaaaag tcaaactgga ggccccacca atgattacct ctggggtaac
     3661 agttgttcca ataaggtttg tggccgaatc atttggggta gagctcaaat atgaagcatc
     3721 a
//
LOCUS       DAOSRI010000172         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22519_length_3690_cov_4.048693, whole genome shotgun sequence.
ACCESSION   DAOSRI010000172 DAOSRI010000000
VERSION     DAOSRI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22519_length_3690_cov_4.048693"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PK717_05770"
     CDS             <1..909
                     /locus_tag="PK717_05770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR47206.1"
                     /translation="EVIMPAFTIISCALAVIRLGAVPVLLDAEPDTWNMDVSQIESRI
                     TPRTKVIMPVHIYGHPVDMDPLLAIAKRYNLIVVEDAAEAHGAECRGRKCGSLGHISA
                     WSFYANKILTTGEGGMVLTADKKMAERASSYRNLCFHPSRRFYHTELGHNFRMTNLQA
                     AIGVAQMERIEEFVQIKRRLGVYYREGLSQIEGIKYQIEKPWAKMVYWMYCIELDPLL
                     GMDAEQMMIELGKRGIGTRPFFIGMHEQPVLRDLGLFAGECYPVTERIARQGLYLPSG
                     LSLTEREIDEVVESVSEVVNSRRRKN"
     gene            923..1828
                     /locus_tag="PK717_05775"
     CDS             923..1828
                     /locus_tag="PK717_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOR47207.1"
                     /translation="MKLLKRAVCSLFSRLGLEISLRKKNEAFKELYPQCWHGAGFVYN
                     IFGHLLYQNVTDLINYSGLMDIDLNDYAQMHTDEMAVIIKNVKPGQRVIDIGANIGLY
                     TLLLAKLVGADGRVIAFEPGPVSFGLLTVNTHLNRYRNVTLENRAVTDISRTEYYFTN
                     QAEERTNESGGVVFSSKPNFDHSREMIPVEAVSLDDYFAGRDCNVDYIKIDVEGGEYA
                     ALKGMKNLLNANPSIILTIEFAPYLPLWADIDIQEFLDYIRSYGFEIFDLRKDDRDPV
                     SDEYLMESYPKQSVGKYANLLLKRS"
     gene            1860..3068
                     /locus_tag="PK717_05780"
     CDS             1860..3068
                     /locus_tag="PK717_05780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR47208.1"
                     /translation="METIARNTCRLCGGSALHDVFSLGEQFINDFVPEEKIGQGLKAP
                     LDLVMCDQCSLLQLRHTAPQELLYSRHYWYRSGVTDTMRRALRDITEEIEAMVALKPG
                     DVVLDIGANDGTMLATYTAPGIRRVGCEPANNLVDSLNKNADYVMHDFWNYERYMELA
                     HKWGFGKAKVITAIGMFYDLEDPNQFIKDAQLALADGGVFVAQLMCLAPMIEKNDLGN
                     ICHEHLEYYSLDSLKYLFESNGFEMFKIEENDVNGGSYRIFARHYDGMGMAYREKYTL
                     NDILAFAKRIEENRTRCVDFIKNEVSKGKKIYVYGASTKGNVILQYYGLDYRLITAAA
                     ERSPEKWGKYTIGSWIPIVSEEEARRANPDYFLVLPWAFFSEFYEREVEWRSRGGQFI
                     VPLPEFRVVP"
     gene            3065..3496
                     /locus_tag="PK717_05785"
     CDS             3065..3496
                     /locus_tag="PK717_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR47209.1"
                     /translation="MIPSDKYESIMRVLPILCVDIIVKTESGKYILIKRKNRPLSGRW
                     WVIGGRVLKDEPLEQAAKRKVKEETDLDIKEIRPIGYYEDTFVANPFGTRFRQHSVSL
                     VFLAIVKENQDVKLDYQSLAWKYSKVLPRRFQVKPFGCLQP"
     gene            3530..>3690
                     /locus_tag="PK717_05790"
     CDS             3530..>3690
                     /locus_tag="PK717_05790"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOR47210.1"
                     /translation="MKKALILGVTGQDGSYLADILLNKGYAVYGMIRRSATGNTRNID
                     HLLQNPDIF"
BASE COUNT         1019 a          703 c          994 g          974 t
ORIGIN      
        1 gaagtgataa tgcccgcttt taccatcatt tcctgtgcgt tggcggtgat tcgattggga
       61 gccgtgccgg ttttgttgga tgcagagccg gatacttgga atatggatgt tagccagatc
      121 gaatcacgga tcacaccaag gactaaagta attatgccgg ttcacattta tggacatcct
      181 gtggatatgg atcctctgtt ggcgattgca aaacgttaca atctcatcgt cgttgaggat
      241 gccgccgagg cgcatggcgc cgaatgtcga ggaaggaaat gcgggtcgct gggccacatc
      301 agcgcctggt cgttttatgc aaataagatc ctcacaacgg gggagggggg gatggttctg
      361 acagcggata aaaagatggc tgaacgggcg tcatcttacc gaaatctctg ctttcatcca
      421 tcccggaggt tttaccatac ggaactcggc cataactttc ggatgacaaa tctgcaagcc
      481 gccattggag tcgcacaaat ggaacggatt gaggaatttg ttcaaattaa gagacggctt
      541 ggtgtctatt atcgggaagg attatctcag attgagggga taaagtatca aatcgagaag
      601 ccttgggcaa aaatggttta ctggatgtac tgcatagagc ttgacccgct gctgggtatg
      661 gatgcggaac agatgatgat cgaattaggg aaaaggggca ttggcacgcg gcctttcttt
      721 atcggcatgc atgagcagcc ggtcctacga gatcttggac tctttgctgg agaatgttat
      781 ccggtaacgg aacgtatcgc cagacaaggt ttatatttac cttccgggtt atcccttacg
      841 gaaagagaaa ttgacgaggt tgttgaatct gtgagcgaag ttgttaactc gcgaagaaga
      901 aagaactgag tgggaggctt tggtgaagct gcttaaaaga gcggtatgct cgttatttag
      961 tcgattggga ctggagatat ctcttcgcaa aaaaaatgag gcatttaaag aattataccc
     1021 tcagtgttgg cacggtgccg gttttgttta taatatcttt ggtcatttac tttatcaaaa
     1081 tgtcacggac cttataaatt atagcggtct aatggatatc gatcttaatg attatgcgca
     1141 gatgcatacg gacgagatgg ctgtgattat caagaatgtc aagcccggac agagagttat
     1201 cgatatcggt gccaatattg gattatacac gcttttgctg gcaaagttgg ttggggctga
     1261 cggacgggtt atcgcctttg agccggggcc ggtcagcttt ggcctgctga cggtgaatac
     1321 ccatctgaat cgatacagga atgttacctt ggagaacagg gccgtaactg acattagtcg
     1381 gacggaatat tatttcacca atcaagcaga agaaaggact aatgagagcg gcggtgtggt
     1441 tttttcctct aagccgaatt tcgaccattc acgggagatg atacctgtgg aagctgtaag
     1501 cctggatgat tattttgccg ggagggactg taatgtcgat tatatcaaga ttgatgtaga
     1561 aggaggagaa tatgcggccc tgaagggcat gaagaatttg ctgaatgcaa atccttccat
     1621 catactgaca atagaatttg caccatacct gcctctatgg gctgatatag atattcagga
     1681 gtttcttgat tatattagat cttatggttt cgaaatcttc gatttacgga aggatgatcg
     1741 tgatccggta agcgatgaat atttaatgga gtcgtatcca aagcagtccg ttggcaagta
     1801 cgccaatctg cttttgaaac gatcctgagt actacgaatg taaatataag gagaggcaaa
     1861 tggaaaccat agcccgtaat acctgtagat tgtgcggcgg aagtgctttg cacgacgtgt
     1921 tttctcttgg ggaacaattc attaacgatt ttgttcccga agaaaagatc gggcaaggac
     1981 tgaaagcgcc tctcgattta gttatgtgtg atcaatgttc acttttacag ctccgccaca
     2041 cggcgcctca ggagctttta tactcacgtc attattggta ccggtcgggt gttacagata
     2101 caatgaggcg ggccttgagg gatattactg aagaaatcga agcgatggtc gccttgaaac
     2161 ccggcgatgt agtcctggat atcggagcga atgatgggac gatgctggct acatatactg
     2221 caccggggat ccgtcgcgtg ggctgtgagc ccgccaacaa cctggtggac agcctaaaca
     2281 aaaatgccga ttatgtgatg catgatttct ggaactatga gcgctatatg gaacttgccc
     2341 acaaatgggg ttttggcaag gcaaaggtaa ttactgcgat tggcatgttt tatgaccttg
     2401 aggacccgaa tcaattcata aaagatgcgc agcttgcctt ggctgatgga ggagtctttg
     2461 tggcccaact catgtgtctg gcgccgatga tcgaaaagaa cgatttgggg aacatatgcc
     2521 atgaacatct ggaatattat tctcttgatt cattgaaata tttgtttgaa tcaaatggtt
     2581 tcgaaatgtt caaaatcgaa gaaaacgatg tcaatggagg cagttacagg atttttgccc
     2641 gtcattacga cggtatggga atggcgtatc gtgagaaata cacattgaat gacattttag
     2701 cctttgcgaa acgcatcgaa gaaaatcgca cccgttgtgt tgattttatt aaaaatgaag
     2761 tcagtaaagg aaagaagatt tatgtttatg gagcatcaac gaaagggaat gtgattctcc
     2821 aatattatgg ccttgattac cgcttgatta ctgcggctgc ggaacgtagc cctgagaagt
     2881 ggggtaaata tactatcggc tcatggatcc ctatcgtttc tgaggaggaa gcgagaagag
     2941 ccaatccaga ctattttctt gtgctgcctt gggctttctt tagtgaattt tacgaacgag
     3001 aggtggaatg gcgttcccgg ggggggcagt ttatcgtccc tttgccggaa tttagggtgg
     3061 tgccatgatc ccaagtgata aatacgaaag cataatgagg gtattaccaa tattgtgtgt
     3121 ggatataatc gtcaaaacgg aaagcggcaa gtatatattg ataaaaagaa agaacagacc
     3181 gctcagtggt aggtggtggg ttatcggggg gcgggttctc aaagatgaac cgctggaaca
     3241 ggcagcaaaa agaaaggtta aggaagagac ggatctcgac atcaaggaga tcagaccaat
     3301 agggtactat gaagatacct tcgtcgcgaa tccgtttggc acaagattcc gccaacattc
     3361 ggtcagtttg gtattcctgg ccatcgtgaa ggaaaatcag gatgtgaaat tggactacca
     3421 gagcttggcg tggaagtact cgaaggtact cccccggcgg ttccaggtca aaccttttgg
     3481 ctgtctgcaa ccatgatatg aagcgcatag cgcagggaag gataattaga tgaaaaaagc
     3541 cctgatcctc ggtgtaaccg gtcaggacgg cagttacctc gcggacatat tgttgaataa
     3601 aggttacgcc gtttatggaa tgatcagacg ttcagccacg gggaatacac gcaatatcga
     3661 tcatttgctc cagaaccccg acattttcaa
//
LOCUS       DAOSRI010000173         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22526_length_3688_cov_4.145334, whole genome shotgun sequence.
ACCESSION   DAOSRI010000173 DAOSRI010000000
VERSION     DAOSRI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22526_length_3688_cov_4.145334"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK717_05795"
     CDS             complement(<1..227)
                     /locus_tag="PK717_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOR47211.1"
                     /translation="MAELYARCGLDCSKCDAYIATKNNDTKLKAEIAKKWKAEFGFDA
                     KPEDINCVGCSLEGAHIGYCNECKVRSCANS"
     gene            complement(291..376)
                     /locus_tag="PK717_05800"
     tRNA            complement(291..376)
                     /locus_tag="PK717_05800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(340..342),aa:Tyr,seq:gta)
     gene            complement(448..1017)
                     /locus_tag="PK717_05805"
     CDS             complement(448..1017)
                     /locus_tag="PK717_05805"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOR47212.1"
                     /translation="MKKSLGAKVLAFPTPVWIVGSYGEDDRPNMMCAAWAGVCCSKPP
                     CVYISLRKATLTYGNIMARKAFTVSIPSQKFVREADHVGIVSGRDEDKFKKLGLTPIR
                     SDIVDAPYPSEFEVVLECKLLHTLEIGLHTEFVGEIMDVKADENLLDEKDHLSMEKFK
                     PFVWDTGDASYRAIGEKIGNGFEDGKTIK"
     gene            1112..2428
                     /locus_tag="PK717_05810"
     CDS             1112..2428
                     /locus_tag="PK717_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOR47213.1"
                     /translation="MKKATVMGLGYVGLPLSLVLWSRGFEVYGLDVRERYIKDLCEGR
                     VKMAESFNGEPITSVLKRAVASGRYHPTTDPDLALGYDDQQIILTVGVPLVDGKTYTK
                     DLEAALDTLGNRLRKGALVIARSTVIVGYTRSFIKPRLEERSGLCATEDFDLAFSSER
                     MAENVAFEELIGMVTPVAGLTPRATRRAADLLREMGVKEVLEASSPEAVETAKVFENI
                     ARDVNIALANEYANFCAAYGLDTKEVLSIVRTHKRVGFLHHPGPGVGGHCLPQAMFYL
                     KPTADSVGVGLPVISTARKANDDQPVHVASLAKSWLDVHGKKNGKVALIGLAMKDYST
                     DARLSPAVDLARALLKNGLGVVCWDPIADVESISGLPPCVSTIEEATKGADVVIIAAR
                     QEPLPEKSPAGLKLLSGMSKPGLLLDCRFAFDRAEVIKNGIDYIAL"
     gene            2403..2876
                     /locus_tag="PK717_05815"
     CDS             2403..2876
                     /locus_tag="PK717_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47214.1"
                     /translation="MELITSPSKKTGFWTQIVAFKRRFLSDWARAIEYFVWGLGFAEA
                     ISFIFWATTGSDYAHWIIRMVLSPIFLYLYGVRYMRFVPTQQKENRRLGLLWFLLNVG
                     FDLVVIVVIAGFDFSRVFLKSQPFLILGYIGVLVSPWIMDTKKHKQHKDDTWIGM"
     gene            2920..2994
                     /locus_tag="PK717_05820"
     tRNA            2920..2994
                     /locus_tag="PK717_05820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2952..2954,aa:Gly,seq:tcc)
     gene            complement(3055..>3688)
                     /locus_tag="PK717_05825"
     CDS             complement(3055..>3688)
                     /locus_tag="PK717_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47215.1"
                     /translation="FNNKVYFLPKGSSWLCALLTSRLMWLIIKKTCDVKLGEVFEMFT
                     IYVEQFPIIDPTGDENNPSTPKGRLALLAKKAQEKAFKRLQLRHDLLEMIGDQLGQGK
                     ANEGDDAEEVSEGSDINKWDTLSWNDFVKKAQSPSAFGKAFKEQFAPMTRRLLDLKRL
                     FEEMQPECKQLTEEIHACENEINQIVYGLYELTPDEIKLLEELTGNIYED"
BASE COUNT          893 a          828 c          868 g         1099 t
ORIGIN      
        1 ttggaattgg cgcatgatct taccttgcac tcgttgcaat agccgatatg cgcgccttca
       61 agagaacagc caacgcagtt gatgtcctcc ggttttgcat caaaaccaaa ttctgccttc
      121 cacttctttg cgatttccgc cttcagtttt gtgtcattgt tttttgtggc aatataggca
      181 tcgcattttg aacagtccag accgcacctt gcatatagtt ctgccatttt tttgctcctt
      241 cgattttttg atttttaaaa agaaaaaaga ggggattttt tgagggttaa tggtgggcag
      301 tgaaggattc gaacctccga agacatgagt caacagattt acagtctgcc gcgtttgacc
      361 gcttcgctaa ctgcccacta ctccatgaat atatagcaat cgaggtgttt tcagtcaagg
      421 acgcgattta aaaaacgcca taaacattta tttgatggtt tttccgtctt caaaaccgtt
      481 gccaattttt tcaccaattg ccctgtagct tgcatctcct gtatcccata caaagggctt
      541 gaatttttcc attgaaaggt ggtctttttc atcaagaagg ttctcatctg cttttacatc
      601 cattatttca ccaacaaact cggtgtggag gccaatttcc agcgtatgca ggagcttgca
      661 ttcgagcact acttcaaact ctgatggata tggtgcatcg acgatgtctg atcttattgg
      721 tgttagacca agttttttga acttgtcctc gtctctgcca gacacaatgc cgacgtggtc
      781 tgcttctctg acgaattttt gggatggaat ggacacggtg aatgcttttc tggccattat
      841 gttgccatag gttagtgttg ccttgcgcag cgaaatataa acacatggtg gttttgaaca
      901 gcagactcct gcccatgcgg cgcacatcat gtttggtctg tcatcctcgc catagctgcc
      961 aactatccat accggcgttg ggaaagccaa cacctttgcg cccaaagatt tcttcatgcg
     1021 ttgcctcctt tttatcagac catgataagt ttctgtctcg ctttttcaat cgcccttgaa
     1081 aagaatggcc tcaaaaaata gaattcttgc catgaaaaaa gcgactgtca tgggtttggg
     1141 ttatgttggt cttccgcttt cgcttgtttt gtggagcaga ggttttgagg tttacggcct
     1201 tgatgtcagg gaaaggtaca tcaaagacct ttgtgagggc agggtcaaaa tggccgagtc
     1261 cttcaatgga gaaccaatca cgtcagtcct gaaaagggct gttgcctctg gtagatacca
     1321 tccgacaact gatccggacc ttgccctggg ttatgatgac cagcaaatca ttcttacggt
     1381 tggcgtaccc cttgttgatg gcaaaactta caccaaggac ctggaggccg cccttgatac
     1441 gcttggcaac aggctcagaa aaggtgccct cgtaattgcc aggtcaacgg tgatagttgg
     1501 ctacacaagg tctttcataa aaccgcgttt ggaagaacgt tctggtcttt gcgcgacaga
     1561 ggattttgat ctcgcattct cttcggaaag aatggccgag aatgtcgctt ttgaggagct
     1621 tatcggcatg gtaacgccag tggctgggct tactcccagg gcaacccgta gggcagcaga
     1681 ccttctcagg gaaatgggtg taaaggaagt gcttgaggcc tccagcccag aagcggtgga
     1741 aacagccaag gtttttgaaa atatcgcaag ggatgtcaac atagcccttg caaatgagta
     1801 tgcaaatttc tgtgcggcct atggcctgga cacaaaagaa gtgctttcaa tagtgcgcac
     1861 ccacaagagg gtcggtttcc ttcatcatcc tgggccaggg gttggcggtc actgccttcc
     1921 acaggcaatg ttttatctaa aaccgacggc agacagtgtc ggagttgggt taccggtcat
     1981 ttcaacagcc agaaaggcca acgatgatca gcctgttcat gttgccagcc tcgcaaaatc
     2041 atggcttgat gtgcatggca aaaagaatgg gaaagttgcc ctgatcggac ttgcaatgaa
     2101 ggattacagc actgatgcaa gactcagccc ggctgttgat cttgccagag cacttctcaa
     2161 aaacggactt ggtgttgttt gttgggaccc gattgccgat gttgaaagca tctccggctt
     2221 gccaccatgt gtttcgacaa tcgaagaggc gacaaaaggt gccgatgttg ttataatagc
     2281 tgctagacag gaaccgctac cggaaaaaag ccctgctggc ttgaaactgc tatctggcat
     2341 gtcaaaaccg gggttgctcc tggattgcag atttgccttt gacagagcgg aggtcataaa
     2401 gaatggaatt gattacatcg ccctctaaaa agactggttt ttggacacaa attgttgctt
     2461 ttaagagaag atttctgtct gattgggcca gggcgattga atactttgtt tggggacttg
     2521 gtttcgcaga ggcgatctct tttattttct gggcaacaac cggttctgac tatgcacact
     2581 ggatcataag aatggttctc tcaccgattt ttctttatct gtatggtgtg agatacatga
     2641 ggtttgtccc aactcagcaa aaggagaatc gcaggctggg acttctctgg tttctcctca
     2701 atgtaggttt cgatcttgtg gtcatagttg tcattgcagg gtttgatttt tcaagggtat
     2761 ttctgaagag tcagccgttc ctgattttag gctatatagg cgtgctggtt tcaccgtgga
     2821 taatggacac caaaaaacac aagcaacaca aggatgatac ctggattggc atgtgaacct
     2881 tgacaaggta gccaacctgg gctaattttt aatggccatg cgggtgtagc tcagtggtag
     2941 agcattagcc ttccaagcta atggtcgcgg gttcaagtcc cgtcgcccgc tccataagaa
     3001 aacacaaaag ggtcagattt ttctggccct ttttatttta ttccatcttg tttgtcaatc
     3061 ttcgtatatg tttccggtca actcttccag gagttttatt tcatcaggtg ttagttcgta
     3121 gagcccataa acaatctggt ttatctcatt ttcacatgcg tgtatctctt ctgtgagctg
     3181 tttgcattca ggttgcatct cctcaaagag cctcttgagg tcaagcagtc tcctggtcat
     3241 tggtgcgaac tgctctttaa acgccttgcc aaaggcagat gggctttgcg ctttttttac
     3301 aaaatcattc caggaaagcg tgtcccactt gtttatgtct gatccttcag aaacttcttc
     3361 agcgtcatcc ccttcatttg ccttgccctg gcccagctgg tctcctatca tctcaagcag
     3421 atcatgcctg agctggagcc ttttgaatgc cttttcctgt gcttttttgg ccaaaagggc
     3481 aagcctgcct tttggagtgg atggattgtt ttcatcgcct gtgggatcga tgataggaaa
     3541 ttgttcgaca tatatcgtga acatctcaaa aacttcccct agttttacgt cgcaagtttt
     3601 cttgatgatt agccacatta atctgcttgt taataaagca cacaaccatg atgatccttt
     3661 tggtaaaaaa tacactttat tgttgaaa
//
LOCUS       DAOSRI010000174         3687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22537_length_3687_cov_3.519548, whole genome shotgun sequence.
ACCESSION   DAOSRI010000174 DAOSRI010000000
VERSION     DAOSRI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22537_length_3687_cov_3.519548"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK717_05830"
     CDS             complement(<1..368)
                     /locus_tag="PK717_05830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393440.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOR47216.1"
                     /translation="MISYIFNNSKAGKVGYSLPPSDVPKAELPKGLTNEKELGLPEVT
                     EVEVVRHVTAMAKETYGVDTGFYPLGSCTMKYNPRINEELAALEGFVNLHPDTPIEGC
                     QGALEMMYNLDKYFCAITGM"
     gene            complement(365..1696)
                     /gene="gcvPA"
                     /locus_tag="PK717_05835"
     CDS             complement(365..1696)
                     /gene="gcvPA"
                     /locus_tag="PK717_05835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOR47217.1"
                     /translation="MRYMPLSPDDVREMLAAIGVKTIDDLFAPVPKDVRLQGVLDIPE
                     AMSEVELRRHVRGLADENYDATKSLCFAGGTGYYHYVPSAVNMLSGLPELLTAYTPYQ
                     PELSQGILMAIFEFQTLMTELTGLPISNASLYDGSTAVCEAARMAVNQSGKNVVSISS
                     LVHPSYIDTLKSFASFLGYEIRIIPATGDRIDTSKIADYAKDSACIIIQSPNVSGTIE
                     DVELIAKNTKSSGSYTVQVVAETISFGLLKRPGDLGVDICCGDAQSFGNPLAFGGPYL
                     GFMTCTEEFVRRLPGRIVGETTDRHGNTVLTLTLRPREQDIRREKATSNICTNNSNSM
                     LRALIYLLLMGKKGLSEVARQNYDKAHYFAEKVKSAGLKIFNQNPFFNEVTILLPSKK
                     DNFATNMLQKGFIVSPTEIPGAGKAITVAVTEMLTKEEIEAFVEALKEVAA"
     gene            complement(1705..2073)
                     /gene="gcvH"
                     /locus_tag="PK717_05840"
     CDS             complement(1705..2073)
                     /gene="gcvH"
                     /locus_tag="PK717_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOR47218.1"
                     /translation="MKVYYTKTDEYVKVDGDTAIVGITEYAQHHLGDIIFVDKIAVGK
                     QVKKEGILTAIESVKAATDVFSPVSGEIVAFNENVSKTPAIINQSAEGDGWIAKIKLA
                     NPGEVNSLLSPEDYSKIHAE"
     gene            complement(2089..2451)
                     /locus_tag="PK717_05845"
     CDS             complement(2089..2451)
                     /locus_tag="PK717_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47219.1"
                     /translation="MNNIELYIEIDDCWVGQCGPEFYIGLLPSTMDKIGNVTFVDRIP
                     IGKILSKGAIIGILETSKLGDWPLKSPVNALVLAFNDKLNKNPSLCTASDMDENWVVK
                     CKIQGISTISDLGEAKTR"
     gene            2566..2985
                     /gene="gcvH"
                     /locus_tag="PK717_05850"
     CDS             2566..2985
                     /gene="gcvH"
                     /locus_tag="PK717_05850"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOR47220.1"
                     /translation="MPKIGDYNLPDDLYYHKEHFWVKKDGNVAIVGIDEFGTKLAGEI
                     SFIELPMEDDEVSQNDQVGSLETGKWLGKIYSPVSGTVIAVNEDAVDDPSAINKDPYN
                     NWLFKVEMKDAGELGNLYGGDKIAPWLENEIATRGKK"
     gene            2972..>3687
                     /locus_tag="PK717_05855"
     CDS             2972..>3687
                     /locus_tag="PK717_05855"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOR47221.1"
                     /translation="MARSDKTRRAISLALFFLFLLQFPLLSSKAAGPPDLKEFKSYLV
                     LQSDGTATVKQDLTFLETESRDAINKFGPFDSGHRIKDQYLLEGSQKISASLISLGDN
                     FYKAEFGSETVPGKTYTFHWEYAIDRDITGNSRQGTRDFGWAPIQWSVPIEKIIVQVV
                     LPVELSSNINKPEQVTDAIVNATGLQTNESNKSNFDRFIFFPTPDERTGKNYLSVHAE
                     KDNAVAEEKAYLELFFPEKY"
BASE COUNT          981 a          835 c          833 g         1038 t
ORIGIN      
        1 tccattccag ttatggcgca gaaatacttg tcaagattgt acatcatctc cagggcgccc
       61 tggcaacctt ctattggcgt gtccggatgg aggttgacga agccctcgag tgcggccaat
      121 tcctcattta tccttgggtt gtacttcatg gtgcacgagc caagcggata aaaacctgtg
      181 tcaacaccgt aggtttcctt ggccattgct gttacatggc gtaccacttc aacttcagtt
      241 acctctggaa ggccaagttc cttctcattt gtaaggccct ttggtagctc agcttttggt
      301 acatcggatg gcggaaggct gtatcccacc tttcctgcct tggagttgtt aaaaatgtag
      361 ctgatcatgc agccacctcc ttgagcgcct caacaaatgc ctcgatctct tcctttgtaa
      421 gcatctcggt aacagccacc gttatggcct tgcctgctcc tggtatttct gttggggaaa
      481 caataaaacc cttctggagc atattggttg caaaattgtc cttctttgat ggaaggagga
      541 ttgtgacttc gttgaaaaac gggttctggt tgaaaatttt gagtcctgca gattttacct
      601 tttctgcaaa ataatgggcc ttgtcatagt tctgtctggc cacttcggaa agtccttttt
      661 tacccattag aagaaggtag atcaatgcac gaagcatcga gttgctgttg ttggtgcaaa
      721 tatttgaggt cgctttttct cttcttatgt cctgctccct cggcctgagt gtgagggtga
      781 gaactgtgtt gccatgtctg tccgtggttt caccaacaat tcttcctggg agtctcctca
      841 caaactcttc ggtacatgtc atgaagccaa gatatggccc gccaaatgca agcggatttc
      901 caaaggattg cgcgtcacca cagcagatgt caacaccgag gtcacccgga cgctttagga
      961 gtccaaatga aattgtttct gcaacaacct ggacagtata cgaacctgaa gactttgtgt
     1021 tttttgcaat cagctcaaca tcctcgattg ttcccgaaac attcgggctc tgaattatta
     1081 tgcaagctga atcctttgcg taatcggcaa tcttggaagt gtcaatcctg tcgcccgtag
     1141 ctggaataat ccttatttca taaccaagga acgacgcgaa ggatttgagt gtgtcgatat
     1201 acgaggggtg cacgagagag cttatcgata caacattttt cccagactgg ttcacggcca
     1261 tccttgccgc ttcacagacg gcagttgagc catcatagag cgacgcattt gagattggaa
     1321 ggccagtaag ctcggtcatt aatgtctgga attcaaaaat agccatcaat atgccctgtg
     1381 agagctctgg ctggtaggga gtataggcag tcagaagctc aggaagaccg gaaagcatgt
     1441 ttacagcaga cgggacgtag tggtaatatc ctgtaccgcc agcaaaacac agtgactttg
     1501 tggcgtcata gttctcatcg gccagaccac gaacatgtct ccggagttcg acttcggaca
     1561 tggcttctgg tatatccaga acgccctgca atctgacatc ttttggtact ggtgcaaaga
     1621 gatcatctat tgttttaacg ccaatggcag caagcatttc ccggacatcg tccggggaca
     1681 atggcatata gcgcatttaa ggcctcactc tgcgtggatt tttgagtagt cttccggtga
     1741 aagaagtgaa ttgacctcgc ctgggttggc gagtttaatc tttgcgatcc agccatctcc
     1801 ctcagctgat tggttgatga ttgccggtgt tttcgaaaca ttttcgttga atgcaacaat
     1861 ttcacctgag actggcgaaa aaacgtctgt tgcggctttt accgactcga tagcggtcag
     1921 gatgccctct tttttgacct gttttccaac agcaatcttg tcgacaaaga ttatgtcacc
     1981 caaatgatgc tgggcatatt cggtgatacc aacaatggct gtgtcgccgt ctactttaac
     2041 gtattcatct gttttcgtgt aataaacttt catattttcc tcctgtgttt atcgcgtctt
     2101 ggcttcgcca agatcagaga tcgttgatat gccctggatt ttacacttta caacccagtt
     2161 ttcatccata tcacttgcgg tacaaagtga tggatttttg ttcaatttat cgttaaaagc
     2221 aagtacaaga gcattaacag gcgacttcaa aggccagtca ccaagttttg atgtttccaa
     2281 tattccgatt attgcacctt ttgacaatat cttgccaatg ggaatcctgt ctacaaaggt
     2341 cacgtttccg atcttatcca tggttgaggg aagtagacca atataaaatt ccggaccgca
     2401 ctgaccgacc caacagtcgt caatttctat gtacaattca atattgttca cgcttcgcat
     2461 tctagcccag ggcctctttc tgtcaaggtt cctttccatg ttgacgtggt gaaaacagcg
     2521 ttgtaaaatt catcagtatt taagtgaatt caaaggaggt atcgtatgcc aaaaattggc
     2581 gactacaatc tgccagatga cctttattac cacaaggagc atttctgggt taaaaaagac
     2641 ggcaatgttg caattgttgg gatagatgaa tttgggacaa agctagctgg agaaatatct
     2701 tttatagaac tcccgatgga agatgatgaa gtttcccaga atgatcaggt tggctcactt
     2761 gaaaccggga aatggcttgg aaaaatatat agccctgttt ctggaacagt aatcgcagtc
     2821 aatgaagatg ctgtagatga cccatccgca atcaacaagg acccttacaa taattggtta
     2881 ttcaaggttg aaatgaagga tgcaggagaa cttggaaacc tttatggtgg agacaagata
     2941 gcaccttggc ttgaaaatga gattgcaact cgtggcaaga agtgacaaaa caaggcgggc
     3001 catttcgctc gccttgttct ttcttttttt attacaattc ccgttgctat catcaaaggc
     3061 agctgggccc cctgacctaa aagaattcaa gagctatctt gtccttcagt ctgacggcac
     3121 cgcaacagtc aagcaggacc tcacgttcct ggagaccgag agcagggatg ctatcaacaa
     3181 gtttgggcct ttcgacagcg ggcacaggat aaaagaccag tatctcctcg aagggagcca
     3241 aaagatttct gcttcgctta tttccctggg agataatttt tacaaggcag aatttggttc
     3301 tgaaactgtt cctggaaaaa catacacctt ccattgggaa tatgctattg atcgcgatat
     3361 taccggcaac tcaaggcaag ggacaaggga ttttggctgg gcaccaatcc agtggagcgt
     3421 gccaatcgaa aagatcattg tccaggttgt tcttccagtt gagctctcaa gcaatatcaa
     3481 caaacctgaa caggtcacag acgccatagt aaacgcaaca ggattgcaaa caaatgaatc
     3541 gaacaagtca aattttgaca gattcatctt cttccccact ccggacgaaa gaacaggcaa
     3601 aaactatctc tcagtccatg cagagaaaga taatgccgtt gctgaggaaa aagcttattt
     3661 agaacttttc tttccagaaa aatattt
//
LOCUS       DAOSRI010000175         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22663_length_3672_cov_3.683716, whole genome shotgun sequence.
ACCESSION   DAOSRI010000175 DAOSRI010000000
VERSION     DAOSRI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22663_length_3672_cov_3.683716"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK717_05860"
     CDS             complement(<1..747)
                     /locus_tag="PK717_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOR47222.1"
                     /translation="MELLVLGIGLLGLLVAALLVFLVRKSDPGDSVMVGISNAIRQGA
                     FAFLTKEYQYMFLVAVPFAILIGFVISPYVALSFSIGALFSVLAGFVGMNVATLANTR
                     TTQAAKTRGEGRALDMAFSGGAVMGLVVSSLGIIGITALFLVFRDTLHSSETASILVG
                     FSVGASFVALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGD
                     IAGMGADLYESYVGAIVSALVLATLADGTFD"
     gene            complement(772..846)
                     /locus_tag="PK717_05865"
     tRNA            complement(772..846)
                     /locus_tag="PK717_05865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Val,seq:cac)
     gene            complement(900..2312)
                     /locus_tag="PK717_05870"
     CDS             complement(900..2312)
                     /locus_tag="PK717_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47223.1"
                     /translation="MKYRFGSFFLALVVLFCTPVQSANIARMGFGWCYCTIRGTLVTT
                     MNDRVIVTDSNMGRMFLFTLDGHFAGHLGNADECKKDPTFTPSSVCYGGNNIYACCLQ
                     EHAVKRYDHFGALQEKVTSKELGFDGKNPISMAFGTNYFYVVFEGGKIASFEKDWSLV
                     KTIDFEATSCVFKQDKLYVTGKGQVVVFNEELEKLEELGSQGKGLGQLDNPSALSVDE
                     NGNVWVADTGNDRVQVFYKSGGFAVWGQTASSLGQENPGEDPISGRQSPFKPTSIDVS
                     DDFFYVAQPDPGQAWAVPKSLVSRKWKNSSLSLVIFEVSKKTYSKYVLPVWRIQQALY
                     PKALVCVVETEGDEKLVLDGQIFEKGPALKNLLDQPQVLIDELASCIDGKNLQTVQIK
                     IEDGQAVAMAPNPDCVMVSFEVPGSMSPLIVKKLNTAKPRLDGSIRLPIPKPSAPNLI
                     YVCLLNADGSCIGAEVVRGN"
     gene            complement(2293..>3672)
                     /locus_tag="PK717_05875"
     CDS             complement(2293..>3672)
                     /locus_tag="PK717_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47224.1"
                     /translation="YISTFKGNIRAVSIKNGYVVWSSPIDGPIMGRMAVFAGKLYAST
                     WSKRVVCLTQAAGEPIWQIRTDQNFSGGLAVSRSGLLALPDIGKGLCLNPSKGSTLWT
                     MPIGGLSRSAPVSDGERIYYTLTDGTIRAIHISSGNQLWSVDLKGEIWSSPCVSGKRV
                     LAAAADQPLACVDSYSGMLLWKSQVDDYLDSAPVADGSNVYLGAWDGKVYCVEMKTGR
                     RIWSQVTSDIIQASPSVSNGGVVVGSWDDKIYMLDHYTGGIRWVHTVGDNVSKTVSIE
                     GQKIYFPQHDTVTCLDARNSATLFQKKLDSSIASELAVGKEGIFALSDSGQLYCMDKE
                     TGATIWSVFVSDSHSAKTFCLLENAICAVIGPTIKILSRTDGKLIWEYDSAVELSQPI
                     ITSGRLIFTTSTGSLLCFGPSPSNEVVTLSEEKPQEENKDLVVPITRQEAIQRAASTK
                     TTPDEVPIW"
BASE COUNT          969 a          946 c          831 g          926 t
ORIGIN      
        1 gtcgaatgtg ccatctgcca gtgtcgccag cacaagtgcc gagacgattg caccaacata
       61 cgactcgtag aggtcagcac ccattcctgc aatatcacct acgttgtcgc cgacattgtc
      121 agctatcacg gcagggtttc tggggtcatc ttccggtatt ccagcctcga ccttgccaac
      181 aagatctgca cccacatcgg ccgctttggt gtatatacca ccgccaactc tggcaaaaag
      241 cgcaacgaac gaggcaccaa cagagaagcc aaccagtatg ctggccgttt cagaagaatg
      301 cagggtgtct ctgaaaacaa gaaagagggc agttattcca attattccaa gagaagagac
      361 cacaaggccc attactgcgc cgccagagaa ggccatgtct agcgctcttc cctcacccct
      421 tgtttttgcg gcctgcgttg tcctggtgtt ggcaagcgtg gccacattca tgcctacaaa
      481 accggctaga acagaaaaca gggcgccaat agaaaacgaa agggcaacat acggggaaat
      541 gacaaaacct ataaggatcg caaatggaac tgccacaaga aacatgtact ggtattcctt
      601 tgtgagaaag gcgaaagcgc cctgcctaat tgcatttgat atcccaacca tgacagaatc
      661 gccagggtcc gatttcctga ccagaaaaac aaggagggcc gcgaccaaaa gccccaataa
      721 accgatgccc aacaccagta gttccaaagg agcctctttt atttttgaga atggtgggcg
      781 aaacaggatt cgaacctgtg accccttccg tgtgagggaa gtgctctacc gctgagccat
      841 tcgcccgcct tgtttagata ttctattgcg agacttagtt ctgtcaactt aaactggcgc
      901 taattgcctc tcacaacttc agctccaatg caggagccat cagcattcag cagacataca
      961 taaataaggt ttggtgcact tggttttgga atagggagcc taatggagcc atcgagcctt
     1021 ggtttggcgg tatttaattt tttgacaatc aaaggtgaca tcgagccagg cacttcaaaa
     1081 gaaaccataa cacaatcagg atttggtgcc atagcaacgg cttgtccgtc ttcaattttt
     1141 atctggactg tctggaggtt tttcccatca atgcaagatg ccagctcatc aatcaatact
     1201 tgtggctgat caagaaggtt cttaagggct ggcccttttt caaagatttg cccatcaagg
     1261 acgagcttct cgtcgccttc tgtttccaca acacaaacaa gagcttttgg atacagcgcc
     1321 tgctgtatgc gccagactgg caggacgtat tttgaataag tcttttttga tacctcaaaa
     1381 atgaccaggg acagcgagga attcttccat tttctcgaga cgagactctt tggaacggcc
     1441 caagcctggc ctgggtccgg ctgggcgaca tagaaaaaat catccgaaac gtcgatggat
     1501 gttggcttga agggtgattg cctgccactt atggggtctt ctcccggatt ttcctggcca
     1561 aggctactgg cagtctggcc ccacacagca aaacccccgg atttgtaaaa cacctggacc
     1621 ctgtcatttc cggtgtctgc cacccacaca tttccatttt catcaactga caacgcagac
     1681 ggattgtcaa gctgaccgag ccccttgccc tgcgagccca attcctccag tttttcaagc
     1741 tcttcgttga aaacaaccac ctggcccttg cccgtgacat agagcttatc ctgtttaaaa
     1801 acacagcttg tggcctcaaa atcaatggtt tttacaagtg accagtcctt ttcaaatgag
     1861 gcgatctttc caccttcaaa aacaacataa aaatagtttg tgccaaaagc catgctgatg
     1921 ggatttttgc catcaaaacc cagttccttt gaggtcacct tttcctggag cgcgccaaaa
     1981 tggtcatacc gttttacagc atgttcctga aggcagcagg cgtatatgtt gttcccgcca
     2041 tagcaaactg aagatggggt aaatgtcgga tccttcttgc attcatcagc attcccaagg
     2101 tggccagcaa aatgtccatc aagcgtgaaa aggaacatcc tgcccatgtt tgaatccgtg
     2161 acaatcaccc tgtcgttcat tgtggtgaca agggtgcccc tgatggtgca ataacaccac
     2221 ccaaatccca ttcttgctat gtttgcagac tggaccgggg tgcaaaacaa caccaccagc
     2281 gctagaaaaa agctaccaaa tcggtacttc atcgggcgtt gtctttgtgc ttgcggccct
     2341 ctggattgcc tcttgccttg ttattggcac taccaaatct ttattttctt cctgaggttt
     2401 ttcttctgac aaagtaacaa cttcatttga aggtgacggg ccaaagcaca ggagcgaccc
     2461 agtggatgtt gtaaatatga gccttcctga tgttattatt ggttgggaaa gctccacagc
     2521 ggaatcatac tcccagatca atttgccatc tgttcttgag agaattttga ttgtagggcc
     2581 aattacggca cagattgcgt tctcaagcaa gcagaaagtt ttagcgctgt gagagtcaga
     2641 aacaaagact gaccatatcg ttgctccagt ctctttgtcc atgcaataga gctggcccga
     2701 atcggaaagg gcaaagatgc cttccttacc aacagcaagc tcagaggcta tggagctgtc
     2761 aagtttcttc tgaaataatg tggcactgtt tcttgcatca agacacgtga ccgtatcatg
     2821 ctgcgggaaa tagatcttct ggccttctat tgaaacagtt ttacttacat tgtcgccgac
     2881 agtgtgcacc catcttatac caccggtata gtggtcaagc atgtaaatct tgtcgtccca
     2941 ggaaccaaca acaacgccac cattggaaac acttggcgag gcctggatta tatctgaggt
     3001 gacctggctc cagattctcc tgccagtctt catttcaaca caatatactt ttccgtccca
     3061 cgcgccaaga tagacgttcg aaccatctgc aactggtgcg gagtcgagat aatcatcaac
     3121 ttgcgatttc cagaggagca tgccagagta ggaatctaca caggccagcg gctggtcggc
     3181 tgctgctgcc aggaccctct ttccggagac gcatggcgaa ctccagattt cacccttgag
     3241 gtcaacactc cagagttgat tgccagatga aatgtgtatt gccctgattg ttccgtctgt
     3301 gagcgtgtaa taaatacgtt ctccgtctga tactggtgcc gatctggaga gaccacctat
     3361 tggcattgtc cacaatgttg agccctttga aggattcagg caaagccctt tgccaatatc
     3421 gggaagcgca aggagcccgc tcctcgaaac agcgagtcct ccagagaaat tttgatctgt
     3481 tcttatttgc caaataggtt caccggcggc ctgggtcaaa cacacaaccc tctttgacca
     3541 ggttgaagca taaagttttc cggcgaatac ggccattctg cccattatcg ggccatcaat
     3601 aggtgatgac cagacaacat aaccattctt tatcgagaca gccctgatgt tgcctttgaa
     3661 agttgaaata ta
//
LOCUS       DAOSRI010000176         3650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_22844_length_3650_cov_4.540195, whole genome shotgun sequence.
ACCESSION   DAOSRI010000176 DAOSRI010000000
VERSION     DAOSRI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22844_length_3650_cov_4.540195"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2959
                     /locus_tag="PK717_05880"
     CDS             <1..2959
                     /locus_tag="PK717_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOR47225.1"
                     /translation="LQSGISDTKMFLVATYAPTKVRGDTQPIFDSLFGVDGVTRIQMG
                     PLDYNETISYLRSLLASPDFPEQIAMYFFESSQGNILRAKELVKLSLVDGSLSIGAGG
                     RWSFNERKFAETKRIGETRLLIPKIIEGLDVDELTLLKCIAVLGGKSKLVDLVKISKS
                     AKLFKSTEARHLSKLIFSLLSKGLMKRKFEAGGSFWSLSHGIYAEMLLQTTQVGLRKD
                     IYDAVYQALSSSVEDGCNVERDTKLAQLALKGSDPVAAFKSVPQAFSACMRQFALNEA
                     KFYLSKLIQMIPDGEGEYHQNCVEKLAFCDLGIMAPQEALDRFERIDSLAPRIKAGKA
                     ISAVMSKSHKAGHYLQETVPFIDEIPDKILARSLVLSYLNFLYENDPPSAISFSINRA
                     VTTDDLFLKVSCLLVASRLQTAMADFDSAETSCTDAVKFASQSGDANLQAKVALWQIQ
                     LAQMKGENEKIARLIRKSAGLINASWDIKTKAEYYKLASNFFINEKEVDNALSHLRMW
                     VWAQTKIGNSKELARALFELGVALDAKGLIKEAQTTIERSRRVAEEISDQQTVGRALV
                     YTGDYYLLSNLADQALVSFERAEKILGDVQDKEYLCKVYEKLGKMWLSSSEIERARKY
                     AKLLKDLVQDSSNQVQETTYLKLSGALAAFQEKFEKAEECYLKAQDIFERQKIWRELN
                     LLKIELADIFVKQGEYFRALTKLSEARLYFEEQGSGKEIKRIRNAELSIDKELGKYGE
                     DYRNLRMLLEISKALSQIEDVDELLPMIVDMAIKVSGAERGFVMLLTPSGKLEFSIGR
                     NQKKEDLTKERFDFSTTVTDIALSERKLVCITDTATDEKFRAKESIVGLSLRSIMCAP
                     LHVGDDVLGLIYVDSQVPTFYFSRKNAEFFEALCSHAAIALHQARLHHQSIEDVRLFE
                     ENRATRELEKQKKEFVFKFFEKISGPMNQVYDILDKIAGNIVHTEDLTALSNQAKNNI
                     VLIKDMIDQSMKS"
     gene            2973..>3650
                     /locus_tag="PK717_05885"
     CDS             2973..>3650
                     /locus_tag="PK717_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR47226.1"
                     /translation="MIGRTLGNRYKIYDKVGGGGMAEVFLARDTKTGEIVALKILRDQ
                     YTEGTDYVERFQREAKSAMKLSHPNICMVKDFGNDDQTYYMVLEFIEGKTLSSVIEEK
                     GPLPVDVAVSYIKQAALALGEAYRSGIIAHRDVKSQNIMVNPSGQIKMMDFGIAKSRD
                     FATMTTAGSFVGTPEYMSPEQAQGGKVDSRSDMYSLGVVLYEMLAGEVPFEADTPWGV
                     LNMHISKE"
BASE COUNT         1123 a          700 c          859 g          968 t
ORIGIN      
        1 tcttcagtcg ggaatatctg atacaaaaat gtttttagtg gcaacttatg caccaacgaa
       61 ggtaaggggt gatacccagc cgatatttga ttcccttttt ggtgttgatg gtgtaacaag
      121 gatacagatg ggtccattgg attataacga gaccatctcc tatctcagat cgcttctggc
      181 atcaccagat ttcccagaac agatcgccat gtattttttt gaaagttcgc agggaaatat
      241 attgagggcc aaagagctcg taaaactctc gcttgttgat ggctctcttt ccataggggc
      301 tggtgggaga tggtccttta atgaaagaaa atttgcagaa acaaagagaa tcggggaaac
      361 gagattattg attcccaaaa taattgaggg tctggatgtt gatgaactca cccttttgaa
      421 gtgcattgcg gtccttggtg ggaaatcaaa actcgttgat ctggtgaaaa tatcaaaaag
      481 cgcaaagctt ttcaaatcga cagaggcaag acatctctca aaacttatct tctcccttct
      541 ctcaaaaggc ttaatgaaga gaaaatttga agctggtggc tctttttggt cactcagcca
      601 tgggatatac gctgagatgc tgctccagac tacacaggtt gggctcagaa aagacatata
      661 tgatgccgtt taccaggcgc tcagttcttc agtggaagat ggttgcaatg tcgaaaggga
      721 tacgaaatta gctcagcttg cactgaaagg ctctgatccc gttgccgcgt tcaaaagcgt
      781 cccacaggca ttctcagcat gcatgcgcca gtttgcgctt aatgaggcaa aattctatct
      841 ttcgaagttg attcagatga tacctgatgg tgaaggagaa tatcatcaga actgtgttga
      901 aaagcttgct ttttgtgatt tgggaatcat ggcgccgcag gaagcgctcg acagatttga
      961 gaggattgat tctttggctc caagaatcaa ggcgggcaaa gcaatatcgg cagtcatgtc
     1021 gaaatcccac aaagccggcc actatcttca ggagacggtt cccttcatag atgaaatccc
     1081 ggacaagatt cttgccagga gtctcgttct gtcttatctg aattttcttt atgaaaacga
     1141 tccgccatca gccatttctt tttccatcaa cagagcagtt accacagatg atctcttcct
     1201 gaaggtttca tgccttcttg ttgcttccag actccaaacg gccatggccg attttgactc
     1261 ggcagagacc tcatgcaccg atgctgtaaa gtttgcttca cagtcagggg atgccaatct
     1321 ccaggcaaaa gttgcccttt ggcagattca gttggcacaa atgaagggtg aaaatgaaaa
     1381 aatcgccagg cttataagaa aatcggctgg cttaataaac gcaagctggg atatcaagac
     1441 taaagctgaa tattacaaac ttgcatcaaa ctttttcatc aatgaaaaag aggtcgataa
     1501 tgcgctttca catttgagaa tgtgggtgtg ggcgcaaacc aaaattggta actcaaaaga
     1561 gcttgcgaga gcgctgtttg agcttggcgt cgcgcttgac gcaaagggcc taataaaaga
     1621 ggcccagaca acgattgaaa gatcgcgaag agttgctgag gaaatttctg atcagcaaac
     1681 ggtgggcaga gctttggttt atactggaga ttattacctt ttaagcaacc ttgctgacca
     1741 ggcactggtc agtttcgaaa gggctgaaaa aatccttggc gatgtccagg acaaggagta
     1801 tctatgcaag gtctatgaaa aacttggtaa aatgtggctc tcatcgtcag aaattgagag
     1861 agcaagaaaa tatgcaaaat tgctcaagga ccttgtgcag gattcttcga accaagtcca
     1921 agaaacaact tatctgaaat tgagtggtgc ccttgccgct ttccaagaaa aatttgagaa
     1981 agcagaagaa tgctatctca aagcacagga tatttttgag agacagaaaa tctggcggga
     2041 gctaaatcta ctaaagattg aattggctga catttttgta aaacagggtg aatactttag
     2101 ggcactgacg aaattgtcag aagccagatt gtattttgag gaacagggtt ctggcaaaga
     2161 gataaagaga ataaggaatg cagaactttc gattgataaa gagcttggca aatacggtga
     2221 agattaccgt aatttacgca tgctcctcga aatatcaaag gccttatcac aaattgagga
     2281 tgtggatgaa cttttgccga tgatagttga tatggctatt aaggtttctg gtgcagaaag
     2341 gggttttgtg atgctcctta cgccatcggg caagcttgaa ttctcaatag gaagaaacca
     2401 gaaaaaggaa gatttgacaa aagaaagatt tgatttttca accacagtaa ctgatattgc
     2461 tctttcagaa agaaagcttg tatgcatcac tgatactgcc actgatgaaa aattcagagc
     2521 caaagaatct attgttgggc tctccctccg ttcaattatg tgtgcaccac tccatgttgg
     2581 tgatgatgtg cttgggctaa tatacgttga tagtcaggta ccaacattct atttttcaag
     2641 aaaaaatgcc gaatttttcg aagcactctg ttcacatgcg gctattgcgt tgcaccaggc
     2701 cagactgcac catcagtcga tagaagatgt cagacttttt gaagagaacc gtgcgaccag
     2761 agagcttgag aaacaaaaaa aagaatttgt attcaaattt ttcgaaaaga tctcaggacc
     2821 aatgaatcag gtttatgaca tcctcgacaa gatagccggt aatatagtgc atactgaaga
     2881 tttaactgcc ctttcaaacc aggcgaaaaa caatatcgta ttaataaaag acatgattga
     2941 tcaatccatg aaatcatagg aaaataggcg aaatgattgg aagaacctta ggtaacaggt
     3001 ataaaattta tgacaaggtt ggcggaggtg gaatggccga agtttttttg gccagagaca
     3061 ccaaaacggg tgaaattgtt gcgttaaaga tcctcagaga ccaatataca gagggtactg
     3121 attacgtcga gcgtttccag cgtgaagcca agtctgcaat gaagctttcc catccgaata
     3181 tttgtatggt caaagatttt ggaaatgatg atcagaccta ctatatggtt ttggaattta
     3241 ttgagggaaa aacactttcc agcgtcatag aagaaaaagg gccattaccg gttgacgttg
     3301 ctgtttctta tataaaacag gctgcacttg cgctgggtga ggcatacagg tcagggatta
     3361 ttgcccacag agacgtcaag agccaaaaca taatggtcaa tccaagtggc cagatcaaga
     3421 tgatggattt tggcattgca aaatccagag attttgcaac gatgaccact gcaggctctt
     3481 ttgttggaac acctgaatac atgtcacccg aacaagccca gggtggcaag gtagattcaa
     3541 gatcggacat gtattccctt ggcgtagtac tctatgagat gcttgcaggt gaggtacctt
     3601 ttgaggctga tacaccatgg ggtgtcctta atatgcatat ctccaaagag
//
LOCUS       DAOSRI010000177         3629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23020_length_3629_cov_3.454952, whole genome shotgun sequence.
ACCESSION   DAOSRI010000177 DAOSRI010000000
VERSION     DAOSRI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23020_length_3629_cov_3.454952"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..174
                     /locus_tag="PK717_05890"
     CDS             31..174
                     /locus_tag="PK717_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47227.1"
                     /translation="MSFGENIQVSFLVREDKINTVSMLLKEALGGEEPFSPVERRMIF
                     ESI"
     gene            complement(181..495)
                     /locus_tag="PK717_05895"
     CDS             complement(181..495)
                     /locus_tag="PK717_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47228.1"
                     /translation="MIKEVRFDIGCENLEKMLEFYKKLGAKEISKEDDFIALEIGNVK
                     FALWKAEEDQQGVGLTFVSDDYEKEKALLKNEKAVIEEWEDCNMVLFEDPDTNAMSLL
                     GE"
     gene            608..2470
                     /locus_tag="PK717_05900"
     CDS             608..2470
                     /locus_tag="PK717_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR47229.1"
                     /translation="MKEFLLKVLRKPSFWIGVGSLIMIGLIFYFYSFITTGFIPDAIL
                     EITSRPERATVTYNSKYAGETPYTFKSTKTLTGVVKISLPGYNTITINLDKTIIEPGD
                     HKKIRIDLSLDTSLTVTSTPPGANIYIDGEQWGSTPYTFEGLISAGQHKIRVELLSQC
                     YGEQTKDVVVDAKLFSKVHFDLEKLIMVSVSSTPTGAKVICGPDDWGVTPLVKCVKPG
                     KYQLMLSKQGLVDEEKDIEVKEDMSVYVRMYDPESYKKKDSYAVDADQPETEIFAYPK
                     RANGELFGQMVAMGKSPAFLSKSEIQAKAGITDAPASYIVFGLKDGFSPGVTELFESG
                     ETIFPMSQLTIFSKGIQKDHKSFPSHSGHPYSNVSPDGTFSIDASKDKATLKDTASGA
                     SYELYLEKEYDYFNNTFTFSPDSSKLWYFRNNKGVRELIERDLSSQEEKVLDKVDSKE
                     HKNVPMDFIFSCLTNIAYDSSSGKLLYFVPGASEKLTLASTKPGFGGRNIIDQDLVPG
                     APQVDSYKLWMVSPDVLKIQGIFPGSVLWEGLYHLSSRQPIKFKIRTDPDPSGKVGMI
                     KQLDSGMTMFPSCALVTEKDGNLVIGVNLYNKLAALIVYSPIKRDWEFVWLRVD"
     gene            complement(2430..>3629)
                     /gene="ligA"
                     /locus_tag="PK717_05905"
     CDS             complement(2430..>3629)
                     /gene="ligA"
                     /locus_tag="PK717_05905"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR47230.1"
                     /translation="KYPYGADGVVIKVDEVALQQELGATSHEPRWAIAFKFPPEEAET
                     VIREILPSVGRTGAITPLAVFDPVRLEGSTISRASLHNEDEVKKLGVMVGDHVVVRKA
                     GSVIPEVMRVIESKRTGQEKPFVMPKHCPVCGADTLREEGSAFTRCTNASCPAQVKER
                     ILHFASRDNANIDGIGDALVGQLVNSGIVKSIADLYFLTKEDLLKQERMGDKLATKIL
                     SNIESSKKAGLSRILSGLGIMMVGKVSAKAIAARFKNVDDLMGASEDELRVVEGVGEK
                     VSHSIAVFFEQEQNRELIERLKKAGVVLSEEVKEQPEGPLAGQTIVLTGVLESLTRGE
                     AEKLVEDNGGKVSSSVSKKTSFVVAGADARSKLDKAMELGVRVLTEKEFLELLSQPST
                     RQTPSLF"
BASE COUNT          954 a          938 c          798 g          939 t
ORIGIN      
        1 tcgaagacga tgtcccggag agcctgagtc atgagctttg gtgagaatat ccaagtttcc
       61 tttctggtga gggaggacaa aataaacact gtttcgatgc tgctgaaaga agcacttggg
      121 ggagaagagc cattctcccc tgttgaacgc aggatgattt ttgaatctat ctagctaagg
      181 ctactcccca agcagagaca ttgcgttggt gtccgggtcc tcaaaaagca ccatgttaca
      241 gtcttcccat tcctcaatga ctgctttttc atttttcagg agtgcctttt ctttctcata
      301 gtcgtcgctg acaaaagtca atccaactcc ctgctggtct tcttcagcct tccacagggc
      361 gaacttcaca ttgccaattt cgagagcgat aaaatcatct tctttggaaa tttcttttgc
      421 gccaagtttt ttgtaaaact cgagcatctt ttcaaggttt tcgcaaccga tgtcaaatct
      481 gacttctttt atcatttttc tctccttttc tttctagtgg agtttccgct gaaaaccgcc
      541 caatgtcaag agattgtgtt gaccttgtgt tcttagatgg taatatcttc ttcttgagga
      601 ggcattgatg aaggaatttt tgttaaaggt cttgaggaaa ccgtcattct ggattggcgt
      661 tggctcactt attatgatag gtcttatatt ttatttttat tccttcatca ccacaggttt
      721 tatacctgat gccatcctcg agataacctc cagacccgag agggcaacag tcacatacaa
      781 ctcaaaatat gcaggtgaga cgccatacac attcaagagc acaaaaaccc tgacaggcgt
      841 agtaaagatt tcgctgcccg gctacaacac aatcacaatc aacctggaca aaaccatcat
      901 cgagcctggc gaccacaaaa aaataagaat cgacctctcg cttgacacaa gtcttacggt
      961 aacaagcaca ccacccggcg ccaacattta tattgatggt gaacagtggg gctcaacacc
     1021 ctacactttt gaaggcctga tatccgccgg acaacacaaa ataagggtag aactcctctc
     1081 gcaatgctat ggcgagcaga caaaagatgt tgtagtagac gcaaaacttt tctcaaaagt
     1141 acactttgat cttgaaaaac tcataatggt gagcgtctca tccactccaa caggcgcaaa
     1201 ggtaatctgc ggcccggatg actggggagt aacaccccta gttaaatgcg taaaacccgg
     1261 aaaataccag ctcatgcttt ccaagcaggg ccttgttgat gaggaaaaag acattgaggt
     1321 caaagaggac atgtcggtct atgtcagaat gtatgatccg gaatcttaca agaaaaaaga
     1381 ctcctacgct gtagatgcag accagcccga aaccgaaatt tttgcctatc caaaaagggc
     1441 caacggggaa ctgtttggcc agatggtggc aatgggaaaa agccctgctt tcctttcaaa
     1501 atctgagata caggcaaaag ccggcatcac agatgcgccc gcatcctaca ttgtttttgg
     1561 gttaaaagat ggtttctcgc caggtgtcac cgagctcttc gaatccggcg aaacaatctt
     1621 cccaatgtcg cagttgacga tattctcaaa gggcattcag aaggaccaca aatctttccc
     1681 gagccattca ggccacccat attcaaacgt ttctccggac gggacattca gcattgacgc
     1741 ttccaaggac aaagcaaccc tcaaggacac cgcgtcaggg gccagctatg agctgtacct
     1801 ggaaaaggaa tacgactatt tcaacaacac attcactttc tccccggact catcaaaact
     1861 ctggtatttc cgcaataaca agggtgtcag agagctcatt gaaagggacc tctcttcgca
     1921 ggaagaaaaa gtattagaca aggtggattc caaagagcac aagaacgtgc caatggactt
     1981 cattttctcg tgcctgacca atattgccta tgattcgtca tctggaaaac tgctctattt
     2041 cgtcccgggt gcgtctgaga aactcaccct tgcatcaaca aagccaggtt ttggaggcag
     2101 aaatataata gaccaggacc tcgtcccagg ggccccacaa gtcgacagct acaagctctg
     2161 gatggtttcg ccagacgtcc tgaaaataca gggcatattc cccggctcgg tcctgtggga
     2221 aggtctctat catctctctt caaggcaacc tataaaattc aaaatcagga ccgatcctga
     2281 cccaagcggc aaagtgggaa tgataaagca gcttgattct ggaatgacaa tgttcccttc
     2341 gtgcgctctt gtcacggaaa aggacgggaa tctggttatt ggagtaaacc tttacaacaa
     2401 gctcgcggcg ctgatcgtct acagccctat caaaagagac tgggagtttg tctggttgag
     2461 ggttgactaa gtagctccag aaattccttt tcagtcaaaa cccttacgcc cagttccatg
     2521 gccttgtcga gcttgcttct ggcatccgct ccggcaacaa caaagctggt ttttttggac
     2581 actgacgaag aaaccttccc gccgttgtct tcaacaagct tttctgcctc gccccttgtc
     2641 agcgattcga ggacaccagt caggacaatc gtctggcccg caagcggacc ttctggctgt
     2701 tctttcactt cctcggagag caccacaccg gcttttttga gcctttcaat gagctccctg
     2761 ttctgctcct gttcaaaaaa aaccgctatc gagtgtgaaa ccttttcccc gacaccctca
     2821 acgaccctca actcgtcctc gcttgcaccc attaggtcat caacgttttt gaagcgggcg
     2881 gcgatggcct ttgcggagac ttttccaacc atcattattc caaggccgga gagtattctt
     2941 gaaagtccag cttttttgga tgattctatg ttggaaagga tttttgtagc aagcttgtcg
     3001 cccatccttt cctgtttaag caggtcctct tttgtgagga aatagagatc agcaattgat
     3061 tttacaatcc ccgaattaac gagttgcccg acaagggcat ctccgatgcc atcaatgttt
     3121 gcgttgtccc tggatgcaaa gtggaggatc ctctccttga cctgtgcagg acatgaagca
     3181 ttcgtgcacc ttgtaaaagc agaaccctct tcgcgcaggg tgtctgcacc acagaccgga
     3241 caatgctttg gcattacaaa cggcttttcc tggcccgttc tttttgattc aatgaccctc
     3301 atgacttcag gtattacaga gccggccttt ctcaccacca catggtcacc gaccatgacg
     3361 ccaagctttt ttacctcgtc ctcattgtgg agcgatgcgc gtgaaattgt ggacccttcc
     3421 agcctgaccg ggtcaaaaac ggcaagcggc gttatggccc cggtcctgcc aacagaaggc
     3481 agtatctccc tgatgaccgt ctcggcctct tcaggcggga acttgaatgc gattgcccac
     3541 cttggctcgt gcgatgtggc gccaagctcc tgctggaggg caacctcgtc aacctttatg
     3601 acaacgccat ctgcaccata aggatattt
//
LOCUS       DAOSRI010000178         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23057_length_3624_cov_3.441580, whole genome shotgun sequence.
ACCESSION   DAOSRI010000178 DAOSRI010000000
VERSION     DAOSRI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_3624_cov_3.441580"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..951)
                     /locus_tag="PK717_05910"
     CDS             complement(190..951)
                     /locus_tag="PK717_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47231.1"
                     /translation="MKKNTLVATIITALILFGSFAFVFAQQDYHDELILTYPKLKGTK
                     IDKTCLCCHFIQEGTGPRNPYGVMYENEGTSDFAAFEKFDADGDGYTNIQEINAKTYP
                     GDFSDSPSPKKFKKCVTFFTSKDTKTKANITVCVVGGKKVDIAPLGGAAYEKSGTVMA
                     PWKPVVDGLGATAVFNAKDGKGRLDIKKGSKVVAQFWVGKTKGKLNGKDYNCKVAPEL
                     KGKNMFVSTLDLAVKALGATESKITMGKIWNYNLK"
     gene            complement(1226..2746)
                     /locus_tag="PK717_05915"
     CDS             complement(1226..2746)
                     /locus_tag="PK717_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47232.1"
                     /translation="MNSRNFKTTGPALLALSFLVLFSSPDSIAFEPTNQLGLGTSKYK
                     DLYVAVASEGKVMHLDTETGIYLGDFISSKDIGAGSITDVSVCSCGGAIAMLDEAEKT
                     IRLYDYDRNKLWDYNLKGPELSYDKQMVNAIALCQDSDYEHVITTTPEKVFHISPPGQ
                     QSKVFPKETLTGLGNPAGAYMDFIKQVFVCDPDNSRVIKTTEYGNLTASFGQDILSHP
                     VDVSTTEDNDRRIWVADSGTKKVHVFSEDFKHLFDCGDSILQNPLTVICDYLDSGCYV
                     GEITNGKINIHKFDKNGVYLFSIKDLMSLPIQRLSYTKTGSYVLYNRSGENIKLSTPA
                     KKSSGIFMVELRAICESLGFEVSWVAKTRTVIIKKLGTSIKLNVSTAMVDLPDKQIIL
                     DPKPAIIKSRVIVPSTFFEMALDVSSSSSQDTIFFVSPKLLPGQSSPQADIGKDLFEV
                     KCNKCHQAPSPDTKERDAWPEIVKRMASKDPSWITNVDLDRISHYLWGQGKPGQRP"
     gene            complement(2736..3173)
                     /locus_tag="PK717_05920"
     CDS             complement(2736..3173)
                     /locus_tag="PK717_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47233.1"
                     /translation="MKPIHKRTKKGFLGLVRQFGIIIVGLVLVVGVFISMYSLGWLTL
                     KGVQETYGSTETQSAIEMPIGEVVSKCDQIPGDLVKVSGKIMLECKMGTWFRLTDGVD
                     EIFVDLKPANVVLPDRTGKTVTVYGTPKRRGKGCYLEGLRIEF"
     gene            complement(3212..3595)
                     /locus_tag="PK717_05925"
     CDS             complement(3212..3595)
                     /locus_tag="PK717_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47234.1"
                     /translation="MDRRIWAGIIAGAAVVVLVIIGIVAYTNNGGSLASANNCGSSCS
                     GCSAEKSGGCGGESYNAENEQASETTTDLKTLEKEAVNYYAKTYGDSNVTAKTFNAGC
                     CTGVAIIKDGKIIDSLKYKDGQFFK"
BASE COUNT          900 a          800 c          770 g         1154 t
ORIGIN      
        1 ttgtaccctt cagttttgga taggtaagaa tgagttcatc gtggtagtcc tgttgggcgt
       61 aaacataggc aaatgcaccc agtataagcc ctgaaagcac cagaattgcc acaattgacc
      121 ttttcattta cacgtacctc cttggttttt ctatcctaat acgaatttgt ttgtttcagg
      181 ttcagccttt tatttaaggt tgtagttcca gattttaccc attgttatct ttgattcggt
      241 agcgccaagg gctttgactg caagatccaa tgttgaaaca aacatgttct tgcctttgag
      301 ttctggagca actttgcagt tgtagtcttt gccgttgagc ttgccctttg tttttccaac
      361 ccagaattgt gcaactacct tgctgccttt cttgatatca agcctacctt ttccgtcctt
      421 ggcgttgaaa actgcagtcg caccgagtcc atcaacaact ggtttccatg gggccattac
      481 tgttcccgat ttttcatatg cggccccacc aagaggcgct atgtcgactt tctttccacc
      541 aacgacgcaa acagtgatgt ttgcctttgt cttcgtatct tttgatgtaa agaaagtgac
      601 gcatttcttg aatttttttg gtgatggtga atcagagaag tcaccaggat aggtctttgc
      661 gttgatttcc tggatatttg tgtatccatc gccatctgca tcaaattttt cgaatgctgc
      721 gaaatcagag gtgccttcat tctcatacat tacaccataa ggattccttg gccctgtacc
      781 ttcctggatg aagtggcagc aaagacaagt cttgtcaatc tttgtaccct tcagttttgg
      841 ataggtaaga atgagttcat cgtggtagtc ctgttgtgca aaaacaaatg caaatgatcc
      901 gaataaaatc aatgcagtga tgattgttgc aaccaatgta ttttttttca tggacgtcct
      961 cctgatgtag gtgtgctttc ctttatgaga gtttaattcc atggatatca aaacaaggtc
     1021 gtcaagacta agtctcggtt taagcttgaa ttgtgcccct gttttattgg catccatttt
     1081 gcaaaattca ataatttaat ccataattaa acaaaaattc ggaaaataat cttattgaca
     1141 tatgagattt tcagcttttt tgtttatatc gaaaaatgtt aatatgtcta aattaatcaa
     1201 ttgaagaaat aaccaagtaa aattgttatg gcctctgccc tggtttgccc tggccccaaa
     1261 ggtaatgtga tattctgtcc aaatcgacat ttgttatcca ggaaggatct ttggaagcca
     1321 tcctttttac aatctctggc caagcgtctc tttcttttgt gtctggtgat ggtgcctgat
     1381 gacacttgtt gcattttacc tcgaaaagat ctttgccaat atctgcctgt ggggatgact
     1441 ggccagggag cagttttggg gagacaaaaa agattgtgtc ctgagatgac gagcttgaga
     1501 catcaagtgc catctcaaaa aatgttgaag gaacaataac tctcgatttg ataatggccg
     1561 gttttgggtc aaggattatt tgtttgtctg gcaagtccac cattgcggtg ctgacgttta
     1621 attttatgga tgtgcctagt tttttgatta tcactgttct ggtttttgct acccaggaaa
     1681 cctcgaatcc cagcgattca caaattgccc tcagctcgac catgaatatg ccggatgact
     1741 tttttgctgg tgttgagagt ttgatatttt caccagatct gttgtaaagg acatagcttc
     1801 cggttttggt gtaggagagt ctctggattg ggagcgacat caaatctttt attgaaaaca
     1861 ggtaaacacc gtttttgtca aacttgtgga tgtttatctt gccatttgtt atctctccaa
     1921 catagcagcc agaatcgaga taatcacaga ttacagttaa aggattttga aggatgctgt
     1981 ctccacaatc aaaaagatgt ttgaaatcct ctgaaaagac atggactttt ttggtgcctg
     2041 agtctgccac ccatattctt ctgtcattgt cctcggtggt cgagacatcg accgggtggg
     2101 aaaggatatc ttgcccaaat gatgctgtca ggttgccata ttcagttgtt tttatgaccc
     2161 tcgaattgtc aggatcgcag acaaaaactt gtttaataaa atccatataa gcaccggcag
     2221 gattgcctag gccggtgagt gtttctttgg ggaaaacctt actttgttgc cctggtgggg
     2281 aaatatggaa gaccttttcc ggggtggtcg ttataacatg ctcgtaatcg gagtcctggc
     2341 aaagagcgat agcatttacc atctgtttat cataggacaa ctctggccct ttcagattgt
     2401 agtcccagag cttatttcgg tcgtagtcgt agagcctgat ggttttttcg gcttcatcga
     2461 gcatggcgat tgcaccgccg catgagcaca cagaaacatc agttatcgag cctgcgccaa
     2521 tatcttttga tgatatgaaa tcaccaaggt atatgcccgt ttcagtatcc aggtgcatta
     2581 ccttgccctc agaagcaaca gcaacataaa ggtctttata tttgcttgtt ccaaggccaa
     2641 gctggtttgt gggttcaaaa gctattgaat cggggctgga aaagagcacc aaaaaagaaa
     2701 gggccagcaa agctggccct gtagttttga aattcctaga attcaatacg aagaccttcc
     2761 agatagcagc ctttgcctct gcgttttggg gtgccgtaaa ccgttacggt cttccccgtt
     2821 ctgtctggca gcacgacatt ggccggcttc aagtcaacga aaatctcatc aacaccatca
     2881 gtcagcctga accaggtacc cattttgcat tccagcatta tcttgcctga tactttgaca
     2941 aggtctcctg gtatctggtc gcatttgctg actacctcgc ctatcggcat ctcaatggcc
     3001 gactgggttt ctgtgctccc gtatgtctcc tggacacctt tgagcgtgag ccagccaagg
     3061 gaatacatgg aaatgaatac tcccacaaca agcacaagcc caacaataat gatgccgaac
     3121 tgacgcacga ggcccaagaa tcccttttta gttcttttat ggatgggttt catttttgac
     3181 cctttctctg tacgtctgtc agcttggcca atcatttaaa aaactggcca tccttgtact
     3241 tcaagctgtc aatgatcttg ccatctttta tgatggcaac accagtgcag caacctgcat
     3301 tgaaagtttt tgctgtcacg ttgctgtcac catacgtttt tgcgtaataa ttgacagctt
     3361 ctttttccag cgtcttcaaa tctgttgtgg tttcagatgc ttgctcgttt tctgcgttgt
     3421 agctttcgcc gccacatcct ccgctttttt ctgcactgca tcctgagcaa gagcttccgc
     3481 agttgtttgc tgaagcaaga gaccccccgt tgtttgtgta tgcaactatt cctatgatca
     3541 cgaggactac cactgctgct ccggcaatta tcccggccca aattcttcta tccatcgagg
     3601 cctcctttta ggaacaattc taag
//
LOCUS       DAOSRI010000179         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23066_length_3623_cov_4.172646, whole genome shotgun sequence.
ACCESSION   DAOSRI010000179 DAOSRI010000000
VERSION     DAOSRI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23066_length_3623_cov_4.172646"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2180)
                     /gene="fusA"
                     /locus_tag="PK717_05930"
     CDS             complement(93..2180)
                     /gene="fusA"
                     /locus_tag="PK717_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR47235.1"
                     /translation="MQDLEKIRNIGIIAHIDAGKTTTTERILYLSGRIRKMGNVDDGN
                     TTTDYMDQERERGITIVSASTAFEWKGYTVNLIDTPGHVDFTAEVERSLRVLDGVIIV
                     LCGVAGVQPQTETVWRQANRYGVPRLIFVNKLDRVGSNLENVVGKIRERLGANPAVLQ
                     LPIGAEDGFKGVVDIIRERAFVWSSVEDGTNMEVGPVPEDMVDDVKLAKHQLIETLAD
                     SDEEALNIYIETGTLTEDQIEKFVRSGCIKSTITPVLCGSSFRYKGVQPLLDAVISYL
                     PSPLERPPVKGTNPKTGEEIVRHPNVDEPTAAIVFKVVNDPFLGTMCFVRTYSGAVKA
                     GTYILNSTTGRKERVMRCLHLLAEQRLDIPEIPAGDIGGVLGFKEAVTGNTLCDESDQ
                     ILLESVTFPEPVVSMSVEPPTKADEDKLNNALRKLALEDPTFQVKFDQESFQTIISGM
                     GELHLEVMVERIKREFGLQIRVGKPQVAYRETIGKETIARGQYIRQSGGKGQYGDVLL
                     RVSPSERNSDLIFENQVSEAVVPARFVSSIEHGIKDAMEAGPMLSYPITDIKVEFIDG
                     SFHEIDSSDIAFRIAASMALKEAMKKGNPQLLEPIMKLQVITPEEYLGDILGSLSKRR
                     CKVEKLDRQGVSQVIEAMAPMGEMFGYTTELRSLSQGRATHSMEFSHMEIAPPQVRDA
                     VIAAVRGIPWAGQ"
     gene            complement(2193..2663)
                     /gene="rpsG"
                     /locus_tag="PK717_05935"
     CDS             complement(2193..2663)
                     /gene="rpsG"
                     /locus_tag="PK717_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR47236.1"
                     /translation="MPRKKLVLKKRQRLDPIYQNAQIGRLIGKILVEGKRIRAEQIVY
                     GALDTVAQKTGKTPVEVMETVIDKCRPRVEVKPRRVGGATYQVPLEVTVERGEALAIK
                     WIVMFSRKRSGMPMRQRLASEMADSLAGQGGAYKKKEDTHKMAEANKAFAHYRW"
     gene            complement(2671..3045)
                     /gene="rpsL"
                     /locus_tag="PK717_05940"
     CDS             complement(2671..3045)
                     /gene="rpsL"
                     /locus_tag="PK717_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR47237.1"
                     /translation="MPTISQLIRHGRQTPKTKSKTPALQECPQRRGVCTVVRTMTPKK
                     PNSALRKIAKVRLSNGIEVLAYIGGIGHSLQEHSVVMVRGGRVKDLPGVRYHIVRGTL
                     DTTGVETRRQGRSKYGAKRPKK"
     gene            3422..>3623
                     /locus_tag="PK717_05945"
     CDS             3422..>3623
                     /locus_tag="PK717_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47238.1"
                     /translation="MVGIIVPYFSFVWQVGNYEHAISIYNVIFCAIGIITYFFVTLTL
                     NYKVRKAYLVGLVIFLMTAYLSL"
BASE COUNT          860 a          891 c          794 g         1078 t
ORIGIN      
        1 tcgttccgac gttgacgtgc ggttttgtgc gctcgtattt ttgttttgcc atatatgact
       61 cctctcttcc ttttggttaa cttttataaa atttattggc cagcccaggg tattcccctg
      121 actgccgcaa tcaccgcatc tcttacttgc ggcggtgcga tttccatatg tgaaaattcc
      181 atcgagtggg tggcccttcc ttgcgagagg gaccttagtt ccgtggtata gccgaacatt
      241 tcacccattg gtgccatagc ttctattacc tgcgatactc cctggcgatc aagtttctct
      301 accttgcatc ttcttttgga aagcgagccg agaatatcac cgaggtactc ttccggcgta
      361 atgacttgca gcttcatgat tggttcaaga agttgcggat tgcccttctt catggcttct
      421 ttcagcgcca ttgatgcagc aattctgaat gcgatatctg aagaatcaat ttcatggaag
      481 cttccatcaa taaactcaac tttgatgtca gttattggat aactgagcat cggacctgct
      541 tccatggcat cttttatgcc atgttctatc gaagaaacga atctggccgg tacaactgct
      601 tcagaaacct ggttttcaaa aatcaggtca gagtttcgct ccgaaggtga aaccctcagg
      661 agtacatcac cgtactggcc ttttccgcca ctctgcctaa tgtactggcc cctggcaatt
      721 gtttccttgc caatcgtctc acgataggca acctggggct tgccaaccct tatctgaagg
      781 ccaaactcac gctttatcct ctcgaccatt acttcgagat ggagctcgcc catacccgag
      841 atgatggtct gaaaggattc ctgatcaaac ttgacttgga acgttggatc ttcgagagca
      901 agttttctta gcgcgttatt aagcttatct tcatcggctt tggttggcgg ctctacagac
      961 atcgagacga caggttcagg gaaagtaact gattcaagaa gtatctgatc gctttcatca
     1021 caaagggtat tgcctgttac ggcctctttg aaaccaagca cgcctccaat gtcacctgct
     1081 gggatttcgg gaatgtccaa tctttgttca gccaagagat gtagacatct catgactctt
     1141 tcttttctgc cagttgttga gttcaggatg taagttcctg ctttcacggc acctgagtaa
     1201 gtcctcacaa agcacatcgt tccaaggaac gggtcattaa ccactttgaa aacaatggca
     1261 gctgttggtt catctacatt tggatgacga acaatctctt cgcctgtttt cgggtttgtg
     1321 ccttttactg gaggcctctc aagcggtgaa gggaggtagg aaattacggc atcaagaaga
     1381 ggctggacac ccttgtacct gaacgatgaa ccgcacagga ccggggttat ggtgctcttt
     1441 atacaaccgg acctgacaaa tttctctatc tggtcttctg tcaatgttcc agtctctata
     1501 tatatgttaa gggcctcttc atcagagtct gcaagggtct caatcagttg atgtttggca
     1561 agtttgacgt cgtcaaccat gtcttcagga acaggaccaa cttccatgtt tgtaccgtct
     1621 tcaacagatg accaaacaaa agccctttcc cttataatgt caacaacccc tttaaagcca
     1681 tcttcggcac cgattggcaa ttgaagcact gctggattgg cgcctagcct ctcacggatt
     1741 ttacccacta cgttttcaag gttcgaaccc accctatcaa gtttgtttac gaaaataagg
     1801 cggggcaccc cataacgatt ggcctgacgc caaaccgttt cagtctgggg ctggacaccg
     1861 gcaactccgc acaacacaat gatcacaccg tcaagcacgc gaagcgacct ttccacttct
     1921 gctgtaaagt ccacatggcc aggggtgtca atcagattga ccgtgtaccc cttccattcg
     1981 aaagcggttg aggccgagac gattgtgatg cctctttccc gctcctggtc catgtaatcg
     2041 gtggttgtgt tgccgtcatc aacattgccc atttttctga ttcttccgga aagatagagt
     2101 atacgctctg tggtggtggt ttttccggcg tcgatgtggg caatgatgcc gatgtttctt
     2161 atcttttcaa ggtcctgcat aaaaaaaatt cactaccagc ggtagtgggc aaaggcttta
     2221 ttggcctcgg ccattttgtg tgtatcttct ttctttttgt aggcaccacc ctgacctgca
     2281 agggaatctg ccatttcgga agcaagacgc tgcctcattg gcattccgga gcgcttcctt
     2341 gagaacataa caatccactt gattgcaagg gcttcgcctc tctcgacggt aacttcgagc
     2401 gggacttggt aagttgctcc accaacacga cgtggtttta cctctaccct tggacggcac
     2461 ttgtcaataa cggtttccat gacctcaact ggagtctttc cagttttctg ggccacagta
     2521 tcaagagcac cgtaaacaat ctgctcggcc ctgatccttt ttccttcaac caggatcttg
     2581 ccgatgagtc ttccaatttg ggcgttctgg tagattgggt caaggcgctg ccttttttta
     2641 agaactaatt ttttccttgg catgcgttcc tcacttcttc gggcgcttgg cgccgtattt
     2701 tgaacgaccc tgacggcgtg tttcaacgcc tgttgtgtcg agggttcccc tgacaatgtg
     2761 atatctcaca ccaggaaggt ccttaacacg tccgcccctt accatgacaa cggagtgctc
     2821 ctgcaaagag tgtccaattc caccgatata agcaagaact tcaataccgt ttgagagcct
     2881 gaccttggca attttcctca aggccgagtt cggcttcttt ggcgtcatgg tgcggactac
     2941 tgtacacact ccgcgacgtt gcgggcactc ttggagagcc ggcgtcttag acttggtctt
     3001 gggagtttgt ctcccgtgcc taatcaattg actaatagta ggcatctatc acctcaaacg
     3061 cataatatat ttaagtacaa tttttttggc cggagtgcta ttctattttc acttatggcg
     3121 cctgcgaaat agggctattc ttcgcaagca agggtcgccc aacaagattg aaagtcaagc
     3181 tatactccaa gcacgaacgg aaattttagt acatatatat tgtttgtcaa gtggtttcaa
     3241 tttcaggatt taatcatttt ctgaatggat ttaaactccc acagaaaatt attgatattg
     3301 caaattatac ctagtttgac gttaaggatg tgaaaatata tcttattgcc gatttacaca
     3361 aatcagttag cataaaacaa ctatggacac gagaaaaaaa ctggcattat tttcgatttt
     3421 ggtggtagga ataatcgtgc catatttttc tttcgtgtgg caggtcggca attatgagca
     3481 tgccatctcc atatacaatg tgatattttg tgcaataggc ataatcacat actttttcgt
     3541 aacactgacc ctcaactaca aggtgaggaa ggcttatctt gtaggattgg ttattttcct
     3601 gatgactgcc tacctgtcac ttg
//
LOCUS       DAOSRI010000180         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23160_length_3611_cov_4.350394, whole genome shotgun sequence.
ACCESSION   DAOSRI010000180 DAOSRI010000000
VERSION     DAOSRI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23160_length_3611_cov_4.350394"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK717_05950"
     CDS             complement(<1..772)
                     /locus_tag="PK717_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47239.1"
                     /translation="MNEENQGDLFGVGQEETQKVQSAINEALTFFKSGKLAEAQKVLS
                     HSMAFVEKSPPAFGFALLQKLCSVDYLRSDKASLVQHLKALGTFLMSSKQFKQAANVF
                     DTTLAFEPDNKEIIFLNAKNTILSGDLPLGVPQLKKVIDCDPTNIEAFALLVKSSASY
                     RPEGALQYVNGYLRLQPDSFDAHNDVVRIYEQLGIQTEAVTLRIKMSALTKDEKQLQE
                     HLSESAKLYPNEPEFHRKMLEIAIASADWEKTDTELANL"
     gene            complement(769..1248)
                     /locus_tag="PK717_05955"
     CDS             complement(769..1248)
                     /locus_tag="PK717_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOR47240.1"
                     /translation="MRKTILVLLALIVLAGCGRREVVFVGAHKTTWGSVGIAAGWKAE
                     PVNKSDVNLYSTEEHNVKVTIRLLPFPKDLIGYIEGQAKLTGREFVSSEDVKLDGGEA
                     KKFTMRAIYAKLNLIEDLYAVKIGDQLLSILVSVPEESMTGYQAEQVANSVRWNGGK"
     gene            complement(1245..2555)
                     /locus_tag="PK717_05960"
     CDS             complement(1245..2555)
                     /locus_tag="PK717_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR47241.1"
                     /translation="MNYKIVIAVAIEVVLIAISIIFFAPLGYMGIEVEPKGAQVKIDG
                     DPAGRSPLYLAFVTPRKYQLEISKDGYKTLNASFNVSRFTKTIQQFKLEKVYSYEILS
                     DPPGAYVILDGKLLQETTPMKIKGLTAGKYLLILQKEGYPSISKVIETSQSKASFLLS
                     FRQGINVEFTSNPPGAELRLDGKKVGITPCIVEGLFAKTYSAELTIEGYEDLKQSVDI
                     SVNGDKVAFTLRKLNPLGIISDPPGQIAYLDGSPAGKTPLIVNIVDGQYEITVGKIKK
                     QVVLPKDQIVKIENPEKAIFELISESGAVQKYQGPSDAVLPKPPNGKYRLAIPIEGGR
                     IYLSEYTFADKPQKVSSYTYSKDGKTTIRSIGGARSIEIVTPKENIELGMGEAEIDIK
                     QIEEKIGKFDTLNIMVGQGVWSAKFTLTKAEIMAGIIFEVEVVR"
     gene            complement(2545..3030)
                     /locus_tag="PK717_05965"
     CDS             complement(2545..3030)
                     /locus_tag="PK717_05965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOR47242.1"
                     /translation="MEQGQRMEFLPTDRSSFDSNSVGSFEWRTFEHPTQANQMFDPNI
                     INPSLTFALKFADGKIAALSSIFDYDQKNGNCQIAVASNVAPGEVFLSLLKHCFGVLN
                     LEKVGVKLAEGSKIMGDLEKIGFVVEAKMRRHAFVNGKYMDVVWMAIVRDDFKRCNNE
                     L"
     gene            3110..3607
                     /locus_tag="PK717_05970"
     CDS             3110..3607
                     /locus_tag="PK717_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47243.1"
                     /translation="MDEKTKSKVVEISIISVAAIMFILPLSFWFIAHDFFGKVALVGK
                     SNFSPIMDRFFLLSVMFFVMYGVSWMNTEIKYRAAKVFGVIALLALLSVPAIQFDNVL
                     IPQSLDQQIRDVTNVWFLNFPSYAVMAITTGMPLGWQVYLTMGFFAIISGALLWDIKT
                     ELAKN"
BASE COUNT          921 a          839 c          740 g         1111 t
ORIGIN      
        1 aaaggttggc caattcagta tcagttttct cccagtcagc gcttgctatg gcaatctcaa
       61 gcattttcct gtggaattct ggttcattag gataaagctt tgccgactct gaaaggtgct
      121 cctggagctg tttttcatcc ttggtcaaag ccgacatttt gattcttagt gttactgcct
      181 cggtttgtat tccaagctgt tcataaatcc tgacaacatc attatgagca tcaaagctgt
      241 ccggctggag cctgaggtaa ccattaacgt attggagggc gccctcaggt ctataagaag
      301 cgctgctctt tacgagaagt gcaaacgcct caatatttgt tggatcgcag tcgatgactt
      361 ttttgagttg tggtactccc aaagggagat cacccgacaa aatggtgttt ttggcattta
      421 aaaaaatgat ttccttgttg tctggctcaa atgcaagcgt ggtgtcaaaa acatttgcgg
      481 cctgtttgaa ttgttttgat gacatcaaaa atgttcccaa cgccttgagg tgctgaacaa
      541 gcgaagcttt gtcgctcctg agataatcga ccgagcagag cttttgcaaa agcgcaaaac
      601 caaatgctgg tggagatttt tcaacaaaag ccatggagtg tgacaaaact ttctgtgcct
      661 cggcaagttt tccagatttg aagaatgtaa gggcttcgtt tatggcactt tgtacctttt
      721 gcgtctcttc ctgccctact ccaaaaagat caccctggtt ttcttcgttc atttccctcc
      781 attccacctg acagagtttg ccacctgttc agcctggtat ccggtcattg actcttcagg
      841 caccgacaca aggattgata gaagctggtc accaatcttt accgcataaa ggtcttcaat
      901 cagattgagc ttggcgtaaa tggctctcat tgtgaatttc tttgcttccc cgccatccag
      961 ttttacatct tccgagctta caaattccct ccctgtgagc tttgcctgtc cttctatgta
     1021 gcctataaga tctttcggga atggaagaag tctgatcgtc acttttacat tatgctcttc
     1081 ggtggaataa aggttcacat cggatttatt gacaggctca gccttccagc cggcagcaat
     1141 cccgacactt ccccaagttg ttttgtgtgc gccaacaaag accacttcac gcctgccaca
     1201 accagcaaga acaatgaggg caagcaaaac aagaatggtc ttcctcacct gacaacctcc
     1261 acctcaaaaa tgatgcctgc cataatctcg gcttttgtga gtgtaaattt ggcgctccat
     1321 accccttgac caaccattat gttaagggta tcgaactttc caatcttctc ttcaatctgc
     1381 ttgatgtcta tttcagcttc gcccatacca agctcgatat tttcctttgg agttacgatt
     1441 tcgatggatc ttgcaccacc aatactcctg atagttgtct ttccatcttt tgagtatgta
     1501 tacgaagaca ccttttgcgg cttgtcggca aaagtgtatt ctgagaggta tattcttccc
     1561 ccttcgattg ggatggccag cctgtacttg ccgtttggtg gtttgggcaa gactgcatct
     1621 gatgggccct gatatttctg gacagcacca ctctcactta tcagctcaaa aattgctttt
     1681 tcagggtttt ctattttaac aatctggtct tttggcaaaa cgacctgttt ttttatcttt
     1741 ccaacagtta tttcatactg tccgtcgacg atattgacga tcaacggagt tttgccggcg
     1801 ggactgccat caaggtatgc aatctggcct ggcgggtccg aaattatgcc gagaggatta
     1861 agttttctca gcgtgaacgc caccttgtct ccatttacac taatgtcaac gctctgcttc
     1921 aggtcctcat atccttcaat tgtaagctcg gcgctatatg tctttgcaaa aagcccctca
     1981 acaatgcagg gtgttatacc aacctttttc ccgtccagac ggagttctgc acctggagga
     2041 tttgatgtga attcaacatt tatgccctgt ctgaaagaaa ggaggaatga tgccttgctt
     2101 tgggacgtct cgatgacttt cgatatcgaa ggatagccct ccttctggag tatcagcaaa
     2161 tacttgccgg cagtcagacc tttaattttc attggcgtag tttcttgcaa gagtttgcca
     2221 tccagaatga cgtatgctcc aggcgggtca gaaagtatct cgtacgaata gactttttca
     2281 agtttaaact gctgtatggt tttggtgaac ctggaaacat tgaaactggc attgagcgtt
     2341 ttgtaaccat ctttacttat ttcaagctga tatttgcgtg gtgtcacaaa agccaaatat
     2401 agtggcgatc ttcctgccgg gtccccatca attttcacct gggcgccctt aggttccact
     2461 tctattccca tgtatcccaa gggagcaaaa aatattattg aaatagctat taaaacaact
     2521 tctatcgcca cagcaatgac aatcttatag ttcattattg cacctcttga aatcgtccct
     2581 tacgattgcc atccagacaa catccatgta cttgccatta acgaaagcat gtcttctcat
     2641 ttttgcttca acaacaaaac cgattttttc aagatcaccc ataattttgg agccttcagc
     2701 gagtttgacc ccgacttttt caaggttcag cactccaaaa cagtgcttca acaatgacaa
     2761 aaagacctcg ccaggtgcaa cattgctggc tacagcaatc tggcagttgc cgtttttttg
     2821 gtcataatca aatattgaag acaaagccgc tattttgcca tcggcaaatt tcaatgcaaa
     2881 cgtaagcgaa ggatttatga tgtttggatc aaacatctgg tttgcttgcg tcggatgttc
     2941 aaaagttctc cactcaaaag aaccaactga gtttgaatca aaacttgatc tgtcggtagg
     3001 caaaaactcc attctttgtc cttgttccat cgtgtgaata atacaacctg aggcaaaata
     3061 tggcaagtaa cgttgaccat ttggccccat aaaatataat ctccctcaaa tggatgaaaa
     3121 aacaaaaagc aaggttgtag aaatttcaat aatctcggta gccgcaataa tgttcatttt
     3181 gccactttct ttctggttta tcgcccatga tttttttggt aaggtggcac tcgtcggaaa
     3241 atccaatttc tcgccaatca tggacaggtt tttcttgctt tccgtgatgt tttttgtgat
     3301 gtatggtgtt tcgtggatga acaccgagat aaaatacagg gccgcaaaag tttttggtgt
     3361 aattgcactt ctggccttgc tttcagttcc ggcaatccag tttgacaacg ttttaattcc
     3421 acaaagtttg gaccagcaaa tcagggacgt caccaatgtg tggtttttga attttccctc
     3481 ttacgcggta atggccatca caacgggcat gccgcttggc tggcaggtat atctcacgat
     3541 gggatttttc gcgataatat ctggtgcact tctctgggac ataaaaacag agctggccaa
     3601 aaattgagtg c
//
LOCUS       DAOSRI010000181         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23274_length_3598_cov_4.716342, whole genome shotgun sequence.
ACCESSION   DAOSRI010000181 DAOSRI010000000
VERSION     DAOSRI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23274_length_3598_cov_4.716342"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PK717_05975"
     CDS             <1..1584
                     /locus_tag="PK717_05975"
                     /inference="COORDINATES: protein motif:HMM:NF028151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOR47244.1"
                     /translation="GNVPVHEEEHQIHSYSMPSKQPAKFTLLGGSDQLSKDLSNARLF
                     HKRAQTVEEITDSIAIIAPDFLKQQTFDGSNNILQALEKKGIKYIVLGPQTEGSIPYC
                     SIVPAEHEIERSKTQSFRFTHVSFSKDIGPFKKDSLIKYFAGDNIIASGALRPYSDAP
                     CVPFASAGAGNGLVCVGFEVEQKGFVTCIELASKISTEPRCHEILCSMLSYLSEDRQS
                     LQAQLVALSDKSSSYLSSLGFTTTKNSATNGVLYVGEADTITLKDIEKLGAIKTILFD
                     RKGFAFLPKQDGVDIKVSQAKPEPAVDLQDMKELDGMTRFSFIEATGFVYDKWRKNFA
                     PLGKTTIEKFTLPKDAKLTWGIKGYFCKVEDLYPQTAFVNMTDWDKGPSNMPLSLLLQ
                     NLGCKKTFNTDRVEIPLSSLKLEGGARHSQEKVAFLTNGKAKFQAKLEPGAKYQLNLA
                     LYQQKAGNENAKLSILLSGVKIGSIEVKSTTEQLYRLEMVAPSGDCEVALVFENDYYK
                     PPQDRNLFLGRLFLTRLTP"
     gene            1632..2798
                     /gene="metK"
                     /locus_tag="PK717_05980"
     CDS             1632..2798
                     /gene="metK"
                     /locus_tag="PK717_05980"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOR47245.1"
                     /translation="MEKRFVASESVCEGHPDKVADQISDAVLDSILKDDPMGRVACET
                     FVTLGMVIVGGEITTKTYVDLSHLVRDVVKDIGYTEDWTGFTYNTVGVLNAIGKQSPD
                     IAQGVDTGGAGDQGMMYGYACNETPELMPMPIHYAHLLAKRLAQVRKEKVLPWLGPDG
                     KTQVVVEYIDGVAKRIDSIVVGAQHSTEILDAADPSTITEVARKQIIETVVVPTIPSR
                     LIDEKTKFYINQTGKFVVGGPVSDTGITGRKIIVDTYGGACPHGGGAFSGKDPTKVDR
                     SASYMVRFVAKNIVAAGLANVCQAEISYVIGKAEPTHFAIRTFGTGKVSDETIAKTVT
                     DLVDLRPKAIIERLNLRRPIYRLTTNYGHFGKELPEITWENTNELAPKLAEKLL"
     gene            2803..>3598
                     /locus_tag="PK717_05985"
     CDS             2803..>3598
                     /locus_tag="PK717_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47246.1"
                     /translation="MKKILVLVSVAIVLAGCGSATPKKGEWSKPAEKPVTDTSPATRD
                     DSFAPGKGMPEIQSELTIEVLPVKGNPVLIKDSTFTVLYGAGPEQEGKNLAKALKDKG
                     IKYIDYLIQPSSDPASYMGAMGLVGEIQIGNLERLIGDENFMRLERAFMGKSMVELLA
                     AGSIWYLNGVSFDVLYPMQDDFASGRVQDRTSVVLVTKGNISFLLTGQIDGTAENKLI
                     QRGKLKKVSAVIVTNGAPEGSMTEGFMQALSPEKVFIVASKDAKPAT"
BASE COUNT         1041 a          909 c          878 g          770 t
ORIGIN      
        1 ggaaacgtcc ctgtgcatga ggaagaacac cagatacact catattcgat gccatcaaaa
       61 cagccagcaa aatttaccct tctcggcggg agcgaccagc tctcgaagga cctgtcaaat
      121 gccaggctgt tccacaagag ggcgcaaact gtggaagaga tcacagacag catcgccatc
      181 attgccccag atttcctgaa acagcaaaca tttgatggaa gcaacaacat acttcaggcc
      241 cttgagaaaa agggcatcaa atatattgtc ctcggcccac agacagaggg ctccatcccc
      301 tattgctcaa ttgttcctgc agagcatgaa attgaaagga gcaaaaccca gtctttcagg
      361 ttcacacatg tttcattctc aaaagacatt ggccctttca aaaaagactc gctaataaaa
      421 tattttgcag gtgacaacat aattgcatca ggcgcattaa ggccgtacag cgatgcgcca
      481 tgcgttccat ttgccagcgc aggggcagga aacggccttg tatgtgtcgg gtttgaagtg
      541 gagcaaaaag gctttgtgac gtgcatcgaa ctagcatcaa aaatatcgac cgagccaagg
      601 tgccacgaaa ttctctgttc catgctcagc tacctttcag aagataggca gtccctgcag
      661 gcacagcttg tcgccctttc ggacaaatca tcatcttatc tgtcttcact tgggttcaca
      721 accacaaaaa acagcgctac aaatggtgtt ctgtatgttg gcgaagccga tacgatcacg
      781 ctgaaagaca ttgaaaaact cggcgccatt aaaaccatcc tgtttgacag gaaagggttt
      841 gctttcctgc cgaaacagga cggagttgac ataaaggtgt cccaggcaaa accggagccg
      901 gcagtcgacc ttcaggacat gaaggagctt gatggcatga cgaggttttc attcatcgag
      961 gcaacgggtt ttgtttacga caaatggaga aaaaactttg ccccacttgg gaaaaccacg
     1021 atagaaaaat tcacactccc caaagacgca aagctgacat ggggcataaa gggctatttc
     1081 tgcaaggttg aggacttgta cccacaaaca gccttcgtca acatgaccga ctgggacaaa
     1141 ggcccctcca acatgccgct gtcgctcctg ctccaaaatc ttggatgcaa aaaaaccttc
     1201 aacactgatc gtgtcgaaat accactgtca tcattaaagc tcgagggtgg cgccagacac
     1261 agccaggaaa aagttgcctt tctgaccaat ggcaaggcaa aattccaggc aaaactggag
     1321 cctggggcaa aataccagct caatcttgcg ctttaccagc aaaaagcagg caacgagaac
     1381 gcaaagcttt cgattttact ttctggggtt aaaattgggt caatagaggt gaaatccact
     1441 acagaacagc tttacagact ggaaatggtc gcaccctccg gcgactgtga ggtggcgctc
     1501 gtttttgaaa atgattatta caagccaccc caagaccgca accttttcct tggcagactg
     1561 ttcttaacta gattgacacc ctgaccgttt atgatataaa ttgcagtcaa tttgtaataa
     1621 agggggatga gatggaaaaa agatttgttg catcagaatc tgtttgcgag gggcacccgg
     1681 ataaggtcgc cgaccaaatc agcgacgctg ttttggattc aattcttaaa gatgacccaa
     1741 tgggtcgcgt tgcctgcgag accttcgtta cactggggat ggtcattgtt ggcggcgaaa
     1801 tcacaacaaa aacctacgtg gatctttcac acctggtcag ggatgttgta aaggacattg
     1861 gctacaccga ggattggaca ggtttcacat acaatacggt tggtgtcctc aacgcaatcg
     1921 gaaagcagtc ccccgacata gcacagggcg ttgacactgg cggggccggt gaccagggca
     1981 tgatgtacgg atacgcctgc aacgaaaccc cggagctgat gccaatgcca atccactatg
     2041 cccacctgct tgcaaagcgc ctcgcccagg tgcgcaagga aaaagtgctc ccctggcttg
     2101 gcccggacgg caaaacgcag gtcgttgtgg aatacatcga tggcgtcgca aaaaggattg
     2161 attccatcgt tgtcggtgca cagcactcga cagaaatcct tgatgctgcc gacccaagca
     2221 caataaccga ggttgcacgc aagcagataa tcgaaactgt tgtcgttcca accatccctt
     2281 ccagactaat tgacgagaag acaaagttct acatcaacca gacaggaaaa tttgtcgtcg
     2341 gtggcccagt ttccgatact ggcataaccg gccgcaagat aatagtagac acctatggtg
     2401 gcgcttgtcc tcatggtggc ggtgcattct ccggcaaaga cccgacaaag gttgaccgct
     2461 ccgcctccta tatggtccgc tttgttgcaa aaaatattgt tgctgcaggg ctagccaatg
     2521 tttgccaagc cgagatctca tacgtcatcg gcaaggcaga gccaacccac tttgcgatcc
     2581 gcaccttcgg cactggcaaa gtcagcgatg aaacaatagc taaaactgtg accgaccttg
     2641 tcgaccttcg cccgaaagcc atcatcgaga ggctaaacct gaggcgcccg atttacaggc
     2701 tcacgacaaa ctatggccat ttcggcaaag agcttccaga aatcacctgg gagaacacaa
     2761 acgagctggc gccaaagctt gcagaaaagc tgctataata aaatgaaaaa aatacttgtt
     2821 cttgtttctg ttgccatcgt cctggctggt tgtggaagcg ccacacctaa aaaaggcgag
     2881 tggtcaaaac cggcggaaaa accggtaacc gacacgtcgc cagcaacaag ggacgacagc
     2941 ttcgctcctg gaaagggcat gccggaaatc cagtcagaat tgacaataga ggtacttcct
     3001 gtaaaaggga accccgtcct gataaaagac agcaccttta ctgtattgta tggcgcaggg
     3061 ccggagcagg agggcaaaaa cctcgcaaaa gccctcaaag acaaaggcat caaatacatt
     3121 gactacctca tccagccgtc ttccgacccg gcgagctaca tgggcgcaat gggcctcgtt
     3181 ggcgaaatac agatcggcaa cctggaacga ctaatcggcg atgaaaattt tatgcggctc
     3241 gaaagggcct tcatgggcaa atccatggtc gagctcctgg ccgcaggatc aatctggtat
     3301 ctgaacggtg tttcttttga tgttctctat ccaatgcaag acgattttgc aagcggaagg
     3361 gtgcaggaca ggacatcggt cgtgctcgtc acaaagggca atatttcgtt tcttttgacc
     3421 ggccagattg atggtacggc agagaacaag ctcattcaga ggggaaaact caaaaaagtc
     3481 tcggcagtca tcgtaaccaa tggcgcgcca gaaggtagca tgaccgaggg tttcatgcag
     3541 gctctgtctc cggaaaaagt gtttattgtt gcaagcaagg acgcaaaacc cgcaaccc
//
LOCUS       DAOSRI010000182         3582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23421_length_3582_cov_3.960590, whole genome shotgun sequence.
ACCESSION   DAOSRI010000182 DAOSRI010000000
VERSION     DAOSRI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23421_length_3582_cov_3.960590"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..737
                     /locus_tag="PK717_05990"
     CDS             180..737
                     /locus_tag="PK717_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOR47247.1"
                     /translation="MAAILALAIFMGGAWLLSPNKVKWQVNFAFREMWRSLTENWGDK
                     WDTVLQMLGFDDTPAGARVAIACTQCDVCAAVINRYFHRYDSKDIKSLLRKTDLGPMI
                     IDIRSVDEYEKGHIPGALPMPLPTLKEDIWSVNRKTPIIIVGNGDEDYRKIGDDIVEK
                     WRFYNTGYLVGGMKAWDGEIEVEKP"
     gene            788..1345
                     /locus_tag="PK717_05995"
     CDS             788..1345
                     /locus_tag="PK717_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47248.1"
                     /translation="MAKKKRSKIPTDRIAELGVVTAAVTVVYIFTGPLIDNIIPYVGC
                     ILRTIAVGVFLSGVRHYAAWELLLINIIASAFYFFFIPCLNSFIGIPAAFVFVATYAS
                     LRRYMQAWLVTTIASILSYGAMIAVLAIWEPSKFYEKFMTALSWSWILLIAIPLLAWW
                     RQRRSNDVGCAGCTMSCESNVFKEQ"
     gene            1347..2306
                     /locus_tag="PK717_06000"
     CDS             1347..2306
                     /locus_tag="PK717_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOR47249.1"
                     /translation="MRTKFVVVFAVMLAIVSSNFIQFAFALPKTCIYESDYMSMDQFG
                     KTSVVHYKCQRVARDIDYCALHSHVDGKKNYELSLIKAEDALNSSMIGCYLHGVDLSY
                     YTLHSMIWSKSDFTKADLSYAMFSSKVLLECNFSDANLEKSDFGYSFAYNANFDNANL
                     TKCNLIGAWLDGSSFKGSLCKETYFVSARLDGVNFDKARFEHIFFMPGQFTKATGMET
                     VNWGDFKVGTEISGSYNEAIQCYDDLAALYDGLGKKDFADKFSDRKAFCQRQLEVLGA
                     KDDEKQLGPHIALVVGLVLVALAIILTMIAILGRRKKSDVHSS"
     gene            2290..>3582
                     /locus_tag="PK717_06005"
     CDS             2290..>3582
                     /locus_tag="PK717_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOR47250.1"
                     /translation="MFIPLERNGKLSLVHQIKQGVINLIASGDLHKGSRLPSTRDLAK
                     TLKVNRNTVVAAYELLSQEGYVVSQVGQGTIVISDHNHGYENKKNQLNDLFFPWGEKF
                     VRNPQAGVHFQIPHLPQSIGRHYKMINNYPPPELFSEPIIKSIARITRRKSLSTVGFR
                     NVSGFPPLVEEIGRRLSLEGVDMDPRGLVITPGSSIANTLVFSLLLEESDTIIVERPT
                     HFAALRWLVWARCRLIEIPMREDGMDLEALKIALQTGKPKLIYTIPTSHNPTGVTTSE
                     AHRRELISIASKYRIPILEDDYQYSLHIGSPKPPLLSALDRSGLVITTSSFSKVIWQG
                     LRLGFVCAPKRVTDQLENLHRILVGPPMFVSQMGLYEYIESGQLDKSIHLMKKILTER
                     QAAFFAAASKYLKHNVRIWSNPGGATVWIETPGMLGDEV"
BASE COUNT         1006 a          776 c          814 g          986 t
ORIGIN      
        1 agcctttggc gctgaggtca ggttcaagac caagaatgaa atgccaaaaa ccccaaatga
       61 tgaagtcata ttcctcatgc caagcacaat taaagtaaaa gaggcctacg ggacaaagta
      121 acttgatgga ccaatcatca aaacaaaaaa agaatctttg ggccagatgg cggccttttg
      181 tggccgccat cctggccctt gccatattta tgggcggggc atggctcttg tctccgaaca
      241 aagtaaagtg gcaagtcaat tttgcattca gggaaatgtg gaggtcactc accgagaact
      301 ggggtgacaa gtgggacaca gtccttcaga tgctcggatt tgatgacaca cctgccggag
      361 caagggtcgc aatagcctgt acccaatgcg atgtgtgtgc tgctgttata aatcgctatt
      421 tccacaggta tgattcaaaa gacatcaaat cgctgctcag aaagactgat cttgggccga
      481 tgattataga catcagatct gttgatgaat acgagaaagg gcacatcccg ggggccttgc
      541 caatgccact tccaacattg aaggaagata tctggtctgt aaacagaaag accccgatca
      601 taatcgttgg taatggtgat gaagactacc gcaagattgg cgatgatatt gttgaaaagt
      661 ggagatttta caatacaggc tatctcgttg gtggcatgaa ggcctgggat ggtgaaattg
      721 aggttgaaaa gccgtgatct ctgaacagcc aaatcaacaa acaattcaat ctccgtccat
      781 cacgagaatg gccaagaaaa aaagaagtaa aattccaacc gacaggatag cagagcttgg
      841 cgttgtgacc gctgctgtga ctgttgtcta tatcttcacg ggccctctta ttgataatat
      901 cattccttat gtcggctgta tcttgagaac cattgctgtt ggtgttttct tgtcaggcgt
      961 caggcactat gctgcctggg aactcctttt gatcaacatt attgcttcag ctttctattt
     1021 cttttttatt ccgtgcctca acagcttcat aggcataccg gcagctttcg tgtttgtggc
     1081 cacatatgct tcactgagaa gatatatgca ggcctggctg gttaccacca ttgccagcat
     1141 tctttcctat ggtgctatga ttgctgttct tgcgatctgg gaaccatcca agttttatga
     1201 aaaattcatg acggctcttt cctggagctg gatacttttg atagcgatac cccttcttgc
     1261 ctggtggagg caaagacggt caaatgatgt tggttgcgct ggatgcacaa tgagttgcga
     1321 atccaacgtc ttcaaggagc aataatttga gaaccaaatt tgtagtcgta tttgctgtca
     1381 tgcttgccat tgtttcttcc aactttattc aatttgcttt tgctcttcca aaaacttgca
     1441 tttatgaatc agactacatg tctatggacc agttcggaaa gacttccgtg gttcattaca
     1501 aatgccagag agtggccaga gatattgact attgcgcact acattcacat gttgatggta
     1561 agaaaaacta tgagctctcc cttatcaaag ctgaagacgc acttaactca tccatgattg
     1621 gctgttacct tcatggtgtt gatctaagct attatacgct ccactcgatg atctggtcaa
     1681 aatcagattt cacaaaggca gaccttagct atgccatgtt ttcatcaaaa gtgttgcttg
     1741 agtgtaactt tagcgacgca aaccttgaga aatcagattt tggctattcg tttgcctata
     1801 acgcaaattt tgacaatgcc aaccttacca aatgcaatct tattggtgcc tggcttgatg
     1861 gctcaagctt caaaggttct ctttgcaagg aaacatactt tgtatcggca aggcttgatg
     1921 gtgtcaattt tgacaaggca aggttcgagc acatattttt tatgccagga cagttcacaa
     1981 aagcaacagg gatggaaact gtaaattggg gtgacttcaa ggtaggcact gaaatatcag
     2041 gttcctataa tgaagcaata cagtgctatg atgaccttgc agcattgtat gatggcctag
     2101 gcaaaaaaga ttttgcagac aagttctctg atagaaaagc tttttgtcaa cgccaacttg
     2161 aagttcttgg cgcgaaagat gatgaaaaac agttgggccc gcatattgcc cttgtggtag
     2221 ggcttgttct ggtggcattg gccatcatcc tgacaatgat agcaattttg ggtagaagaa
     2281 aaaaatctga tgttcattcc tcttgaacgg aatggaaaac tcagtcttgt acaccagata
     2341 aaacagggcg taataaatct tattgcctcc ggtgatcttc acaaaggttc caggcttcct
     2401 tcaacaaggg acttggcaaa aacccttaaa gtcaacagaa acactgtggt ggctgcgtat
     2461 gagttgcttt cccaggaagg ttatgttgtt tctcaagttg gtcagggaac tatcgtcata
     2521 tccgaccata accatgggta tgaaaacaaa aagaaccaat tgaatgatct ttttttccca
     2581 tggggagaga aatttgtaag gaacccacag gctggtgtcc atttccagat accacatctt
     2641 ccccaatcaa ttggcaggca ttacaagatg atcaataatt accctccgcc ggagcttttt
     2701 tcggaaccga taatcaaatc aatagccagg atcactagga ggaagagtct ttcaactgtt
     2761 ggtttcagaa atgttagtgg gttcccgcca cttgttgagg agattggccg caggctctct
     2821 cttgagggtg tggacatgga cccgcgtggc cttgtgatta caccaggctc cagcattgca
     2881 aatacccttg ttttttcact cctccttgaa gagagtgata cgatcattgt tgaacgccct
     2941 acgcactttg cggctcttag gtggcttgtt tgggcaagat gcagactcat tgaaattcct
     3001 atgagggaag acggaatgga cctggaggca ttaaagatag cccttcagac cggaaaacct
     3061 aaactcatct acactattcc gacatcgcac aacccgacag gtgtcaccac ttctgaagca
     3121 cacaggagag aactcatctc tatagcatca aaatacagaa tcccaatact ggaggacgac
     3181 tatcagtata gcctacatat aggatcacca aaaccacccc tgctttccgc tctcgatcgt
     3241 agtggtctgg tcataacaac ctccagtttt tcaaaggtta tctggcaggg attgaggctt
     3301 ggctttgttt gtgcccccaa aagggtcacg gaccaacttg agaaccttca cagaatactt
     3361 gttggtcctc caatgtttgt ttctcaaatg ggactttatg aatacattga atccggccag
     3421 ctagacaaga gcatacatct aatgaagaaa attctcaccg agagacaggc tgcgttcttc
     3481 gctgctgcat caaaatatct caagcataat gtaaggattt ggagcaatcc tggaggagcg
     3541 acagtctgga tagaaacccc tggcatgctt ggtgatgagg tt
//
LOCUS       DAOSRI010000183         3578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23451_length_3578_cov_4.219415, whole genome shotgun sequence.
ACCESSION   DAOSRI010000183 DAOSRI010000000
VERSION     DAOSRI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23451_length_3578_cov_4.219415"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK717_06010"
     CDS             complement(<1..411)
                     /locus_tag="PK717_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR47251.1"
                     /translation="MKTKGIVFLCILLVVSPILQSFPVISIARSQYMLGDRPVPGPLP
                     PDPKASAPESDRQIDIVYGKAGDVELKLDFAKPKLCHDQKVPLVVHVHGGAWRAGDKK
                     GGIDSAESRMFLQLGFAVASINYRLAPRYVFPAQI"
     gene            complement(485..1186)
                     /locus_tag="PK717_06015"
     CDS             complement(485..1186)
                     /locus_tag="PK717_06015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOR47252.1"
                     /translation="MRKPLHKIFTDIPNHYDLVNHLITWGLDKRWRKAAALKCLSKKP
                     QRMLDLCCGTGDLALTVARLADYPIEIRGLDFSQPMLEIAAQKASSLKGKTIAFDQGD
                     ASKMPYENGHFDCVSNSFGFRNLTYNNPQSREHLSEILRVLKPGGRLVVVESSQPRSS
                     FVRFFYHLYMRYYVYWVGVIVSGNRPAYRYLSESTIKYYSPPELEKLLTSAGFREVNY
                     HPMLFGASGIYEAIK"
     gene            complement(1242..2174)
                     /locus_tag="PK717_06020"
     CDS             complement(1242..2174)
                     /locus_tag="PK717_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR47253.1"
                     /translation="MEIATVEPKRKSASGLKVWFMEARPQYLLLPVVLVFVGTAVAWQ
                     NDGMLHMGNAILALLGLVLCHVSVNVLNDYFDYKSGVDLKTIKTPFSGGSGILPAGLL
                     KSKQALWYGLICFVLAVPIGVYFSIIKGWQLLPLLALGAICVLLYTQVILKTDFPEWS
                     PGMGLGILPVLGAYFVQTGFYSWHALVASVPSGLLVLNLLLLNEFPDIEADKIANKRT
                     LPIRIGGKNAAIVYSAFEILVYLWVIGAVIFGQMPVYCLIALLTLPFAIHAIRGSFNY
                     KDLNKILPAMGSNVMAVLLTQALIGVGYILAAVL"
     gene            complement(2270..2560)
                     /locus_tag="PK717_06025"
     CDS             complement(2270..2560)
                     /locus_tag="PK717_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOR47254.1"
                     /translation="MSIKREKQIIGFMMEIYCHGNHKIHDNLCPECKQLLDYANKKLS
                     KCPFGNSKTSCKRCKVHCYEKGMREKVRKVMKYAGPKMLLYHPFAAISHLFK"
     gene            complement(2727..2843)
                     /gene="rrf"
                     /locus_tag="PK717_06030"
     rRNA            complement(2727..2843)
                     /gene="rrf"
                     /locus_tag="PK717_06030"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2915..>3578)
                     /locus_tag="PK717_06035"
     rRNA            complement(2915..>3578)
                     /locus_tag="PK717_06035"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1010 a          878 c          738 g          952 t
ORIGIN      
        1 tatctgggct ggaaaaacat accttggcgc aagcctgtaa ttgatcgagg caacggcaaa
       61 accaagctgg agaaacatcc ttgactcggc agaatctatg ccaccctttt tgtctccggc
      121 cctccaggca ccaccatgca catgaacaac aagaggcacc ttctggtcgt gacacaattt
      181 tggttttgca aaatcaagct tgagctcaac atcgccagcc ttgccgtaaa caatatcaat
      241 ctgtctgtcg gattcgggag cagatgcttt tgggtctggt ggaagcgggc caggcactgg
      301 cctatcaccc agcatatact gactcctagc aattgagatt acaggaaaac tctgcaggat
      361 tggcgataca acaagaagaa tgcacaaaaa aacgattccc tttgttttca tttttccacc
      421 gccttttaaa agatgatacg caagacgaat ggatattgtc acaggaacaa aaaaagcgag
      481 tagactactt gattgcctcg tatatacccg aggcgccaaa aagcatcggg tggtaattaa
      541 cctctctgaa acctgcactg gtcaacaatt tctccagttc tggtggggaa taatatttta
      601 tcgtagattc tgaaagatac ctgtaggccg gcctgtttcc agacacaatc acacctaccc
      661 agtacacata atatcgcatg tataggtgat agaagaagcg gacaaatgaa gatcttggct
      721 ggctcgactc aacaacaacg agcctgccac ctggcttaag tactcttagt atttcagaga
      781 ggtgttctct tgactgggga ttattgtatg tcaggttgcg gaagccaaac gagttgctca
      841 cgcaatcaaa gtgcccattt tcatacggca tctttgaagc atcaccctga tcaaaagcaa
      901 tagttttccc tttcagagaa gaggccttct gggcagcaat ctcaagcatt ggctggctga
      961 aatcaagccc cctgatttct attggataat ccgccagcct ggcaactgtc agggccaaat
     1021 ccccagttcc acaacacaga tcaagcattc tctgtggttt ttttgacaaa cattttaatg
     1081 ccgcggcttt gcgccacctt ttgtccagcc cccatgttat gagatgattt acaagatcat
     1141 aatgatttgg aatgtcagtg aatatcttgt gcaaaggttt tctcacattt tgtttctttt
     1201 ctttttcagc caaattcgtc cttgccttct taattagata gctatagcac tgctgccaga
     1261 atatagccaa ccccaatgag cgcctgggta agcaaaactg ccatgacgtt gctccccatt
     1321 gcaggcaata tcttgttcaa gtccttgtaa ttgaaagaac ctcttatggc atgtatggca
     1381 aacggcaggg tcagcagggc aatcaggcaa tagactggca tctggccaaa tattactgct
     1441 cctatgaccc aaaggtaaac gaggatttca aaagcggaat aaactattgc agcatttttt
     1501 ccaccgatcc tgatcgggag ggtcctcttg ttggcaatct tgtcagcttc gatgtccgga
     1561 aattcattga ggaggagcag gttcaggaca aggagaccgg acggaacaga cgcaacaagc
     1621 gcatgccaag aataaaatcc tgtctggaca aaataggcgc ccaaaactgg cagaatgcca
     1681 agacccatcc caggtgacca ctccggaaaa tcagtcttca aaatgacctg cgtataaaga
     1741 aggacgcaaa tggcgccaag ggcaagcagt ggaagaagct gccagccttt tataatcgaa
     1801 aaatacacgc ctatcggcac tgcaagcaca aaacaaatca aaccgtacca gagggcctgt
     1861 tttgatttca gcaggccagc tggaagtatt ccactaccac cgctaaaagg cgtcttgatg
     1921 gttttgagat caacaccact tttgtaatca aaataatcat ttaggacatt cacgctgacg
     1981 tggcacagaa caagaccaag aagcgcaagt atggcattac ccatatgcag catgccatca
     2041 ttttgccaag caactgcagt gccaacaaaa accaacacta ctggcaaaag caggtattgt
     2101 ggtcttgcct ccataaacca gaccttcaaa cctgaagcac tttttctttt cggttcaaca
     2161 gttgctattt ccatgtttgc cccctatttt cactttccaa aattgcaatg attcatattc
     2221 agacactagt atattttatt atcaataaga ctcttgataa ctagcctagt tacttaaaca
     2281 gatgtgaaat tgccgcaaat ggatggtaca aaagcatctt tggtcccgca tatttcatta
     2341 ctttgcgcac cttttctctc attccctttt catagcaatg gactttgcac ctcttgcacg
     2401 aggtttttga attgccaaaa ggacacttgg aaagtttctt atttgcataa tcaagcaatt
     2461 gtttgcactc agggcaaaga ttatcatgta ttttgtggtt tccatgacaa taaatttcca
     2521 tcataaaacc aattatctgc ttctctcttt ttatggacat aatttatccc aaacaacttt
     2581 catggggata ttttacaatt atcagagaaa ccaaaggata agataggatt attaaatggc
     2641 tatataggaa tgcaatttga gaaaatatgg aagaattttt gaatctttag atggtgtttt
     2701 taaaaaccct agaaacaaaa aaaagacctt ggcaacgtct tactcttccg gaggcctgcg
     2761 cctcgagtac catcagccct gcaggtctta acttccgtgt tcgaaatggg aacgggtgtt
     2821 tcccctgcgg cattgtcacc aagatctttt tttaaaaaac atttttcaaa aacttgggat
     2881 caacactaag aaaactatgt aggaattttt ttaaatagct aagccttcga cctattagta
     2941 cacatcacct caacgcattg ctgcgcttac agcttgtgcc tatcaacccg gtagtcttcc
     3001 ggaggtctta tgccttctag aggcgggaga actcatcttt ggaatggctt cacacttaga
     3061 tgccttcagt gtttatccaa cggaacataa ctacccagcg cctaccactg gtgtgataac
     3121 tggtacatca gaggttcctc tgccctggtc ctctcgtact aagggcagca tccatcaatt
     3181 ctccaacgcc tgcggcggat aaggaccgaa ctgtctcacg acgttctgaa cccagctcac
     3241 gtgcctcttt aatgggcgaa cagcccaacc cttgggacct tatacagccc caggatgaga
     3301 cgagccgaca tcgaggtgcc aaactgcccc gtcgctgtga acgcttggga gcaataagcc
     3361 tgttatcccc gaggtaactt ttatccgttg agctccggcg cttccattcg caaccgtcgg
     3421 gtcactaagc cctggtttcc catctgcttg gtccgtcgac ctatgcagtt aagcctgctt
     3481 atgcctttgc actctttgcg tggtttccaa acacgctgag cagacctttg ggcgcctccg
     3541 ttacctttta ggaggcgacc gccccagtca aactgccc
//
LOCUS       DAOSRI010000184         3529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23873_length_3529_cov_4.164364, whole genome shotgun sequence.
ACCESSION   DAOSRI010000184 DAOSRI010000000
VERSION     DAOSRI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23873_length_3529_cov_4.164364"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK717_06040"
     CDS             <1..794
                     /locus_tag="PK717_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR47255.1"
                     /translation="VKPVLISLCIVGVKIVGSSVVLGLGGAGGDLAPAIFIGAFIGAA
                     FAPIAPAYLPAFVFAGICAQLASAFHAPLTSVLLALEMTGQMTLIVPVVMATSVASLL
                     SIKLSPRSIYQEGLHKRGIQSKLGQFQNPSETKITDVMSSNLITIDQQESVKKAIDLM
                     RNSHLSGLPVLDGKNLFGVITLSDLRSNVTTADLEKPVGSFCSTDLVTITPDAVLSDC
                     VAEMSENEIGRLLVVSPSNPKKLLGIVSKKDLLELATRRYAQIDE"
     gene            825..2087
                     /locus_tag="PK717_06045"
     CDS             825..2087
                     /locus_tag="PK717_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47256.1"
                     /translation="MKSNIFTKAILLVLLCSTIAGCGLYDLPVKRLSRNRIKGELIGY
                     FLNYDKDKHHLVNGIKPITLEESTVYTYDDPGLNSVREIFTVEGKIVSRLVSPKTDLD
                     LYFLHDISNSRYLARYGISGDKIFDVPIPKEITTIIGNNYKRLYYEDDLVFCQTDDKK
                     AVYVYQNGGFANKITFKKPMHFLPRIFMAYDGENLHRFSQTRELKEIQVISLGQVGDL
                     SKTILDTQPYASFEEMPKAISICDGKSVFYFDYDDNDNLFLALKRDIKVLGKSADYRA
                     FPTDKGIYTIEPRIRVSDLFPKPNPKCNYKCYFNLLVPELKDYVPVVTLDSIRHEMTA
                     YPNLFQKKSGRYTKLSILSDFSNQHLLLYNIWPIKDEIISVARCYVDGMQYIHLFCMN
                     GVYLVKFSSFRIEKDKPEFIDMLPRNIP"
     gene            2178..2936
                     /gene="arsM"
                     /locus_tag="PK717_06050"
     CDS             2178..2936
                     /gene="arsM"
                     /locus_tag="PK717_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOR47257.1"
                     /translation="MEKDEIKQVIQKHYGEIAAKNGSGCCGSSCGCGTNITNAQIAKS
                     LGYTDSEIAFASEANLGLGCGNPTGMGSIREGETVLDLGSGAGFDCFLAAKKVGDKGH
                     VIGVDMTQEMIDRAGQNAKKHGFENVEFKLGDIEKLPVESNSVDVIISNCVINLAPDK
                     PKVFSEAFRVLKKGGRMYISDIVLLKDLTPEQKRNEELIAGCVGGALLRDDYLAIVGK
                     AGFEVNVLSEDKDISERQYQNIPLESLKIEAKKV"
     gene            complement(2963..>3529)
                     /locus_tag="PK717_06055"
     CDS             complement(2963..>3529)
                     /locus_tag="PK717_06055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR47258.1"
                     /translation="RQKVSDNITKDLKVTKDQAKEFFESKLLGLSQIVVLYDEKIDGK
                     ELYEPTKKYAESLKAMIGKDKTFADVATMYSEERESGQNGGRMSQLMPKGYLPPQMEK
                     VAFSLKIGEISDVIETNGSFVILKLDYETYAWDYFFTDGGKKPKPNFEVVWKDVVNYL
                     YTIKKIEAENNWYQKYADSIKIDVYLEL"
BASE COUNT         1023 a          708 c          777 g         1021 t
ORIGIN      
        1 ctgtaaagcc tgtgctgatt tcactttgca ttgttggcgt aaaaattgtt ggctcgtcgg
       61 tcgttcttgg gcttggtggg gcaggtggtg accttgcgcc tgcaatattt atcggtgctt
      121 ttatcggtgc cgcttttgcc ccgattgccc cggcctatct gccagctttt gttttcgcgg
      181 gcatttgtgc tcaactggca tcagcttttc atgccccgct cacatcagtt ctgcttgcgc
      241 ttgaaatgac cgggcaaatg acgctcatag ttcctgttgt aatggcaact tctgtggcat
      301 cactcctctc aataaagctc tctccaaggt caatatacca ggaagggctc cacaaaaggg
      361 ggatacagag taaactcggc cagttccaga acccctccga aacgaagatt actgatgtca
      421 tgtcatctaa cctgataaca atagatcagc aggagtctgt aaaaaaagcc atcgatctga
      481 tgagaaacag tcatctctct gggctcccag ttcttgatgg aaagaacctt tttggagtga
      541 taacactttc tgatctaaga agtaatgtca cgacagctga cctggaaaaa cccgttggat
      601 cattttgctc aaccgatctt gtcactataa cacccgatgc ggtcctctca gactgcgttg
      661 ctgaaatgtc tgagaatgag attgggaggc tgcttgttgt gtccccatct aatccgaaga
      721 aactccttgg gattgtttca aagaaagacc ttttggagtt ggccacaagg cgctatgctc
      781 agatagatga atagctaaat ggcaggcagg aaggaaagag caaaatgaaa agcaatattt
      841 ttacaaaagc catcttgctg gttctgctct gttcaacaat tgcaggttgt ggtttatatg
      901 acctgccagt aaaacgattg agcagaaata gaataaaagg cgagttaatc ggatactttc
      961 ttaattatga taaagataaa catcatctag taaatggtat aaaaccaatc acactagagg
     1021 agtccactgt ttacacttat gatgaccctg ggctgaacag cgtgcgggag attttcaccg
     1081 ttgaaggcaa aattgtctca cgactggtat ctccaaagac agatcttgac ttatacttct
     1141 tgcatgatat tagcaattca agatatttag ctagatatgg catttctggt gacaaaatat
     1201 ttgacgtgcc aattccaaaa gaaatcacca caattattgg gaataactat aaaagattgt
     1261 actacgaaga tgatcttgtt ttttgtcaga cagatgacaa aaaagcagtt tatgtatatc
     1321 aaaatggggg atttgccaac aagataacat ttaaaaagcc aatgcatttt cttcccagaa
     1381 ttttcatggc ttatgatggt gaaaatctcc acaggttctc gcagacaaga gagctaaagg
     1441 aaatccaggt aatcagtttg ggtcaggttg gtgatctatc aaaaacaatc cttgatacac
     1501 agccatatgc gagctttgag gaaatgccaa aagctatttc aatatgtgac ggcaaatcag
     1561 ttttctattt cgattatgat gataatgaca atcttttcct tgccttgaaa agagacatca
     1621 aggtgcttgg caagagtgca gattacaggg ctttcccaac agataagggt atttacacaa
     1681 tagaaccgag aatcagggtt tctgatctct ttccaaaacc aaatcctaaa tgtaattaca
     1741 agtgctattt caatctcttg gttcccgaat taaaggatta tgttcctgtg gtgacgcttg
     1801 attccataag gcatgaaatg actgcttacc caaatctatt tcagaaaaaa tctggtcgat
     1861 acacaaaatt gtcaattctg tccgatttta gcaaccaaca tttattgctt tacaatattt
     1921 ggcccataaa ggatgaaata atttccgttg ccagatgcta cgtcgatgga atgcaataca
     1981 tacatctatt ttgtatgaat ggagtatatc ttgtaaagtt cagcagcttc agaattgaaa
     2041 aagataaacc agaatttatt gatatgttgc ctagaaatat tccgtgaaag tgtttgactg
     2101 atactaccac ttagcggaga atttgttaaa acagtttata tcttttaagg tgtccaacca
     2161 ggcaggaggt aataattatg gaaaaagatg aaataaagca ggtcatacaa aagcactacg
     2221 gcgaaatcgc agccaaaaac gggtctgggt gctgtggaag cagttgtggt tgtggcacta
     2281 acatcaccaa tgcacagatt gcaaaaagcc ttggatatac tgattcggag atagcatttg
     2341 cttcagaggc caatcttggc cttggctgtg gcaatccaac tggaatggga agtatcaggg
     2401 agggtgaaac tgtccttgat ttgggttctg gtgctggttt tgactgcttc ctggccgcaa
     2461 aaaaagttgg cgataagggt catgtgattg gtgtggacat gacccaggaa atgattgaca
     2521 gggcgggtca gaatgccaaa aagcatggtt ttgaaaatgt cgaattcaag cttggagaca
     2581 tagaaaaatt gcctgttgaa agcaactccg tcgatgtcat cataagcaat tgtgtcatca
     2641 accttgctcc agacaagcca aaagtcttta gcgaggcctt ccgtgtgctt aaaaagggtg
     2701 gcaggatgta tatttcagac attgttttgc tgaaagacct cacgccagag caaaaaagaa
     2761 atgaagagct gatagcagga tgcgtgggtg gggccctgct gagggacgat tacctggcaa
     2821 tagtgggaaa agcaggtttt gaagtcaatg tcctttcaga ggacaaggat atcagtgaaa
     2881 gacagtatca gaacattcct ctagaaagcc tgaagataga agccaaaaaa gtatagtatt
     2941 ttataagatt ctaaccttgg ccctacagct ccagatatac atctatcttg atggagtcag
     3001 cgtatttctg gtaccaatta ttttctgcct ctatcttctt gatcgtgtaa agatagttta
     3061 caacgtcttt ccagacaacc tcaaagttgg gtttaggttt cttgccgcca tccgtgaaga
     3121 aatagtccca ggcgtaggtt tcgtagtcca gcttcagtat tacaaaactg ccattggtct
     3181 caattacatc cgagatttcg ccaatcttca acgagaacgc caccttttcc atttgtgggg
     3241 ggaggtaacc ctttggcatt agttgcgaca tcctgccgcc attctggccg ctttccctct
     3301 cctcggagta cattgttgcc acatctgcaa aggtcttgtc tttcccgatc atggccttga
     3361 ggctctcggc gtattttttt gttggctcat agagctcctt gccgtcaatc ttctcgtcgt
     3421 aaagcaccac tatttgtgaa agtcccagta attttgattc aaaaaattct tttgcctggt
     3481 cttttgttac ctttaggtct ttagtaatgt tgtctgacac cttttgtct
//
LOCUS       DAOSRI010000185         3526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_23912_length_3526_cov_3.544512, whole genome shotgun sequence.
ACCESSION   DAOSRI010000185 DAOSRI010000000
VERSION     DAOSRI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23912_length_3526_cov_3.544512"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK717_06060"
     CDS             <1..190
                     /locus_tag="PK717_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47259.1"
                     /translation="AARAASMGLLVSPGSFFVPEGAGLQAIRISLIDEDPDGISEAVS
                     ILGKALNPPEGKMVYDGL"
     gene            complement(187..1119)
                     /locus_tag="PK717_06065"
     CDS             complement(187..1119)
                     /locus_tag="PK717_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47260.1"
                     /translation="MKKLICLLVVFLVCSCGSSTNTPDKETIIPVKDEVKDFLPYQYF
                     VPQAKQDEFGFGISIPDGFILSSNDYKASGNITITSKDGATKISMEWKNIKGHTDAKT
                     FLELDMIKDSMSGFSISGEESIEPWSTPFPGEEDITPCQATFAQVQYDREQDGQKFEG
                     RVMVWWYDVPEKNGKSVGLVAIEESKQQYFESILPKLEEIRRSYLRLPSYNEIAGKAQ
                     RAKVEPAKFDSFGKADVEDRLLQYSGGTQPGPGFNTLLKNLTPAYDEETGDNYLVERQ
                     ETPSGLLPNPKRLGMTVKPGIPAKIYSYLLLKQN"
     gene            1229..1843
                     /locus_tag="PK717_06070"
     CDS             1229..1843
                     /locus_tag="PK717_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOR47261.1"
                     /translation="MIFTGYLDDFDLIDFIRFITTMKKSGVLVITGKKSAKIVLSFGK
                     ITGVVVPFEVRNHYKSYIADLKDGYFSLEESHSLNEDEVVVVSQDVSEFLLELTRFRR
                     EPSDPKLYEPNMLFELKPISDIGRLTFNVEEWRIIAALSESRTLKELSERLEIGIARL
                     QWMIYSLEQSGLIKRKRPQAQRERMTQNAITEALKRFIGILKGS"
     gene            1840..2358
                     /locus_tag="PK717_06075"
     CDS             1840..2358
                     /locus_tag="PK717_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOR47262.1"
                     /translation="MTKLKILITGPFSSGKTTFVNALSDIVTVQTEERVWGEDAKIKQ
                     ATTVGLDFGKIQISSDVQIYLFGTPGQDRFEFIWEDMIVGSHGLIVLLDRSDWRSVMQ
                     TKKFIDFYTSRTVVPMVVVANKSDLEDTLPLEFIQAYLDTDPSIGFVEASGKDKSSAR
                     RVLEDMIIRILE"
     gene            complement(2339..2698)
                     /locus_tag="PK717_06080"
     CDS             complement(2339..2698)
                     /locus_tag="PK717_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOR47263.1"
                     /translation="MASVKVLDYLEKMMNTYKELKKKLEMFSASTIKIKGMDGNTNSR
                     TAILDKLLEIDDMLEGLDVKEESLIRLIYLEGITFDKASSMVGISRATAFRMRDKAAT
                     KIYEKKGKICVPIRGCV"
     gene            2783..3298
                     /locus_tag="PK717_06085"
     CDS             2783..3298
                     /locus_tag="PK717_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOR47264.1"
                     /translation="MRIKTPAKALLLIITFVIVGLSTFLYVPKQFDSSSFFFDKNALQ
                     QVINLIRTSKRQIFVEMYSFTHGDVIRELEAKKRAGLDVRVIVDDNPENEALAKARFD
                     VRFEGTPRMFHRKFAIVDEQWIWIGSTNWTYSGFGKNAEVDLLMSSPDHAYNLVRQFE
                     QDWAKAKRRPK"
     gene            3295..>3526
                     /locus_tag="PK717_06090"
     CDS             3295..>3526
                     /locus_tag="PK717_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOR47265.1"
                     /translation="MNYLVLGNGAREHAICWALSKNSDVHAMPGNPGMILDADIVSGN
                     PMNFGEVASVAKKLSCSAVFAGAELPLVEGITD"
BASE COUNT          977 a          703 c          761 g         1085 t
ORIGIN      
        1 tgccgcgagg gctgcttcaa tgggtctcct tgtctcgcca gggtcttttt tcgttccaga
       61 gggagcggga ttgcaagcaa tcagaatctc gctcatagat gaagacccag atggtatttc
      121 cgaagcggtg tccatcttgg gaaaagcgtt gaaccctcca gaggggaaaa tggtttatga
      181 tggtctttag ttttgtttca gcaggagata cgagtatatt tttgctggga tacctggttt
      241 aacagtcatt ccaaggcgct ttgggtttgg aagcaggcca gaaggtgtct cctgtctttc
      301 gaccagatag ttgtctccag tttcctcgtc gtatgccggc gtgagatttt ttaaaagggt
      361 attaaaacct ggtcctggct gagttcctcc actgtattgc aagagcctgt cttcgacatc
      421 cgcttttcca aaagaatcaa attttgctgg ttctactttt gctctctgcg cctttcctgc
      481 tatctcattg tatgatggga gacgtagata acttcttctt atttcttcaa gtttgggcaa
      541 gatgctttca aaatattgct gttttgattc ctctattgcc accaaaccca cagatttgcc
      601 gtttttttca ggaacatcat accaccatac catcaccctg ccttcaaact tttgtccgtc
      661 ctgttcccta tcgtattgca cctgggcaaa agttgcttgg catggtgtga tgtcttcttc
      721 tccaggaaat ggagttgacc agggttcgat tgattcctct ccacttattg agaaaccgct
      781 catggaatct tttatcatat ccagctcgag aaaagttttt gcatccgtat gccctttaat
      841 atttttccat tccattgata tctttgttgc tccatctttt gacgtaatag ttatgtttcc
      901 ggatgccttg taatcattgc tactgagtat aaatccatcg ggtatactga taccaaaccc
      961 aaactcatct tgttttgcct gcggcacaaa atactggtat ggcaaaaaat ccttaacttc
     1021 atctttaact ggaataatcg tttctttgtc aggcgtattg gtggacgatc cacatgagca
     1081 aactaaaaaa acaaccagca aacatattaa tttcttcatg gacatgataa taattgcgtg
     1141 caattttttg acaagtaata gatcgtttct gtcggaaata ttaaataatt gttgacacaa
     1201 atatttcgaa aatgtaaaat attttaccgt gatatttaca ggatatcttg atgattttga
     1261 cctgattgat ttcatcaggt tcatcaccac gatgaaaaaa agtggagtgc ttgtgataac
     1321 tggcaaaaag tcggccaaaa tcgtgctgtc ttttggcaaa atcactggtg tcgttgttcc
     1381 tttcgaagta agaaatcatt acaaatccta tattgctgat ctaaaggacg gatatttcag
     1441 tcttgaagag agtcattcat taaatgagga tgaagttgtg gtagtcagtc aggacgtatc
     1501 ggaattcctt ttggaactta ccagatttag aagagaacct tcagatccaa agttgtatga
     1561 acccaacatg ctatttgagc ttaagcccat ttctgacatt ggcagattga cattcaatgt
     1621 ggaagaatgg agaataatag ccgcactctc tgaatcaaga accttaaaag agttatctga
     1681 aagactggaa atcggtattg ccagattgca atggatgatt tattcactcg agcaaagcgg
     1741 gcttattaaa agaaaaagac cgcaagcgca aagggagaga atgacccaga acgctattac
     1801 agaagcactc aagcggttca taggcatcct aaaaggttca tgacaaaact aaaaattctg
     1861 attaccgggc ctttttcttc gggtaaaaca acatttgtaa atgcgctttc agatatagtg
     1921 actgtccaga ccgaggaaag agtctggggt gaggatgcaa agatcaagca ggccacaacc
     1981 gtgggccttg attttggaaa aatacagatt tcaagtgatg ttcagatata tctttttggg
     2041 acccctggcc aggatcgttt tgaattcatc tgggaagata tgatagtagg ttctcatgga
     2101 ctgatagttc ttcttgatag atcagattgg cgctctgtta tgcaaaccaa gaagtttatc
     2161 gatttttata catcaaggac tgtagttcca atggttgtag tcgccaataa atctgatctg
     2221 gaggataccc tcccattgga gttcattcaa gcgtatctag atactgatcc ttcaattggt
     2281 tttgtggagg cttccggcaa agacaaaagt tctgcaagaa gagttctgga agacatgatt
     2341 atacgcatcc tcgaataggg acacagattt tccccttctt ttcatagatc tttgttgctg
     2401 ccttgtcacg cattctaaaa gcagttgccc ttgaaatacc taccattgaa gatgctttat
     2461 caaaggttat tccttccaga taaatcagcc ttataagact ctcttccttc acatcgagtc
     2521 cttcgagcat gtcatctatc tccagaagtt tatctagtat ggcagttctg ctatttgtgt
     2581 ttccatccat gccttttatt ttgatcgtag acgctgagaa catttccagt ttctttttaa
     2641 gttccttgta tgtgttcatc attttttcaa gatagtccaa aacttttact gacgccattt
     2701 tctccctcct tcaaaaagta acacgacaat agagtgtgct aacttaacca tacagaagcg
     2761 ttgtattgtg taaaatcctt gcgtgagaat aaagaccccg gccaaggccc tgctcttaat
     2821 aataacattc gtaatcgttg gtttaagtac ttttttgtat gttccaaagc agtttgattc
     2881 gtccagtttc ttttttgaca agaacgcttt gcaacaggtc atcaacttga taaggacatc
     2941 aaagagacaa atctttgtcg aaatgtactc cttcactcat ggtgatgtga taagggagct
     3001 tgaagcaaaa aagagggctg gtttggatgt tagggttata gtagatgaca acccggaaaa
     3061 cgaagccctt gccaaagcca gatttgatgt cagatttgag ggaacaccaa gaatgttcca
     3121 ccgtaagttt gcgattgtgg atgaacaatg gatatggata ggatcaacaa actggaccta
     3181 cagtggcttt ggaaagaacg cggaggtcga cttgttgatg tcatcccctg accatgcgta
     3241 taatcttgtc aggcagttcg aacaggattg ggcaaaagcg aaaaggaggc cgaaatgaac
     3301 tatctcgtgc tgggaaatgg tgcgagggaa catgcaatat gctgggcatt gtcaaaaaac
     3361 tcggatgtgc atgcgatgcc tggtaatccc gggatgatcc ttgatgctga tattgtttca
     3421 ggcaacccaa tgaattttgg ggaagtggcc agtgttgcaa aaaagctttc ctgctctgcc
     3481 gttttcgctg gtgccgaact cccgttggtt gaagggataa ctgatg
//
LOCUS       DAOSRI010000186         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24081_length_3507_cov_3.877462, whole genome shotgun sequence.
ACCESSION   DAOSRI010000186 DAOSRI010000000
VERSION     DAOSRI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24081_length_3507_cov_3.877462"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="clpX"
                     /locus_tag="PK717_06095"
     CDS             complement(<1..1008)
                     /gene="clpX"
                     /locus_tag="PK717_06095"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOR47266.1"
                     /translation="MSEEEKDKQQICSFCGQPLSIGVRGMEGNGALICENCINLGSKL
                     LQDESTRKIKRRIGHVPPPEEIHKKLDSHIIGQDQAKKVISVGVYKHYRSVLGFKSTG
                     VEVEKSNIMLIGPTGSGKTLIAKTLAKILNVPFAIADATTLTEAGYVGEDVENVLLGL
                     LRSADWDVDAAQVGIVYIDEIDKIGRKSENPSITRDVSGEGVQQALLKMVEGTVCNVP
                     PQGGRKHPQQQFIQVDTTKILFIIGGMFESLPKIIANRLGKRQVGYNRQPVEQIEESS
                     LLSQVTPDDLIKFGMIPEFVGRFPIVTSVSDLSTDELARILVEPRNSLVKQYETILAA
                     EG"
     gene            complement(1001..1600)
                     /gene="clpP"
                     /locus_tag="PK717_06100"
     CDS             complement(1001..1600)
                     /gene="clpP"
                     /locus_tag="PK717_06100"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOR47267.1"
                     /translation="MNLVPWVIEQTGYGERQYDIYSRLLKDRIIFLGGEMIEDATANS
                     VVAQLLYLDAEDPKKEISLYINSPGGVITSGLAIYDTMQYISAPVSTICIGQAASMGA
                     ILLASGAKGKRFALPHSRIMIHQPAGGAQGQATDIEIQAKEMLRMKKAVNEILARHTG
                     QPLDKIQADTERDFFMESQEAVEYGLVDKVIESVGKQNV"
     gene            complement(1609..2871)
                     /gene="tig"
                     /locus_tag="PK717_06105"
     CDS             complement(1609..2871)
                     /gene="tig"
                     /locus_tag="PK717_06105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOR47268.1"
                     /translation="MQITTNPEGRTTLVGKATFTPDEVSQGFEQVYKDNVSRIKIPGF
                     RPGKAPFKIFATHVGIESMKEDFTEIAARQALKEMLSGKTIRVVGSPTFEVDSFEGDG
                     PLNLTIKIFFIPDYELADPSKVAIEVPKFDVTDTDMTKAIDNIRGRYASMAPVDRKAK
                     KGDFVYFKWSVITDGRASDRKKEELVEVGMEDFVKDFDKNLVGVKSGDTKRVMAEISS
                     GQEPVEIEINIGEIKERILPNLDDDFAKTLHVENVEALKDIVKNELLEQAKDAKEEYI
                     ESMIAKALLEKTNIELPENLVESSVDDEIEKLARDLSKKNITLEMYMEKRAINEEQLR
                     AELTPKATNQAKLDVILDDYSKENSIEVSKEEIDQEIAAISKAMKDARRPAIDQNDEQ
                     VRKNLARIIRRRKTVEYLMGKVQLTEKQ"
     gene            complement(2887..2961)
                     /locus_tag="PK717_06110"
     tRNA            complement(2887..2961)
                     /locus_tag="PK717_06110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2927..2929),aa:Gly,seq:gcc)
     gene            complement(2974..3059)
                     /locus_tag="PK717_06115"
     tRNA            complement(2974..3059)
                     /locus_tag="PK717_06115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3024..3026),aa:Leu,seq:tag)
     gene            complement(3117..>3507)
                     /locus_tag="PK717_06120"
     CDS             complement(3117..>3507)
                     /locus_tag="PK717_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47269.1"
                     /translation="RYRWLLEQKETSGGKVDGSISFMALRGNRLAGAVLAIDRGEYGE
                     ILALGCMPIPKSAEIMAKLVHSSLSGFIKKEVKTIRTTVPMFKGEEIRIFSNLGLVQA
                     FFKPDAILLASDDAWSSLSEKVASSTP"
BASE COUNT          839 a          831 c          727 g         1110 t
ORIGIN      
        1 accctctgct gcaagaattg tttcgtactg ctttactagg gaatttcttg gctccacaag
       61 tatccttgca agttcatcag tgctgagatc tgaaacactc gttacaatcg gaaagcggcc
      121 aacaaattcc ggtatcatgc caaacttgat caagtcgtca ggagtgacct gggaaagcag
      181 tgatgattcc tcaatctgtt caaccggttg cctgttatat cctacttgcc ttttgccaag
      241 cctattggcg attatttttg ggagtgattc gaacatcccg ccaataatga agagtatctt
      301 tgttgtatca acctgtataa actgctgttg gggatgtttt cttccaccct gcggtggaac
      361 attgcaaacg gtcccttcaa ccattttcaa aagtgcttgc tgaacgcctt cgccagaaac
      421 gtctcttgtt attgaagggt tctcagattt tcttcctatc ttgtctatct cgtctatata
      481 aacaatgccg acctgtgcag catcaacgtc ccagtccgcc gacctgagga gtccaagaag
      541 gacgttttcg acatcttcgc caacatagcc agcttcggtg agtgttgttg cgtctgcgat
      601 tgcaaaagga acgttgagaa tttttgcaag tgtttttgct atcaaggtct ttcctgagcc
      661 agttggtcca atgagcataa tattggattt ttcaacctca acgccggttg atttgaagcc
      721 aagcacagaa cggtaatgtt tgtaaactcc aacagatatg acttttttgg cctggtcctg
      781 gccaatgatg tggctgtcga gttttttgtg tatctcctct ggaggaggaa catgaccaat
      841 gcgccttttt attttgcggg tcgactcgtc ctgcaaaagc tttgagccaa gattgatgca
      901 attttcacat atcagggcac cattgccctc catcccgcga acaccaatag acaatggctg
      961 tccacagaaa gaacagattt gttgcttatc tttttcttct tcagacattt tgcttgccaa
     1021 ctgactctat gaccttgtca acgagcccgt actctacggc ttcctgtgac tccatgaaga
     1081 agtccctttc ggtgtctgcc tggattttgt ccaatggctg gcctgtatgt ctggcaagaa
     1141 tttcgttgac agccttcttc atcctgagca tttcttttgc ctgtatttca atgtctgttg
     1201 cctggccctg cgcaccacct gcgggctgat ggatcattat tctggaatgt ggaagagcaa
     1261 aacgcttccc tttggcgcct gaagccagga gaatagctcc catcgaggca gcttggccaa
     1321 tgcagatcgt tgatactggt gcagaaatgt attgcatggt gtcataaatg gcaaggccag
     1381 atgttatcac accgccagga gagttgatgt aaaggctgat ttcttttttg ggatcttcgg
     1441 catcgagata gagcaactgg gccactacag aattggctgt tgcatcttcg atcatttcgc
     1501 cgccaagaaa aattatgcgg tctttaagga gtctgctgta aatgtcatat tgacgctcac
     1561 cgtaaccggt ttgctcaata acccacggaa caagattcat ggttcacctc actgtttttc
     1621 tgtgagctga accttcccca tcaaatactc gacggttttt cttcttctga taattctggc
     1681 gaggtttttc ctgacctgct catcgttttg gtctatggcc ggtcttctcg catccttcat
     1741 tgcctttgag attgctgcaa tttcctggtc aatctcttct tttgaaactt caatggaatt
     1801 ttcttttgaa tagtcgtcga gtataacatc caacttggct tggtttgttg cttttggggt
     1861 tagttcagcc ctgagctgct cctcattgat agctcttttt tccatgtaca tttcaagtgt
     1921 gatgttcttt tttgaaaggt ccctggcgag cttttcaatt tcatcatcaa cagatgattc
     1981 aacgaggttt tctggcaact caatatttgt cttttcaagt aaggccttgg ctatcatgga
     2041 ctcaatatac tcctctttgg cgtcctttgc ctgttcaaga agctcatttt ttacaatgtc
     2101 cttcaaggcc tcaacgtttt caacatgtag tgtcttggcg aaatcatcat caagatttgg
     2161 caatattctt tcttttattt cgcctatatt aatctcaatt tcaacaggtt cttgcccaga
     2221 agaaatctcg gccatgactc tttttgtatc acccgatttt acaccgacaa gatttttgtc
     2281 aaaatctttg acaaaatctt ccatcccgac ttctacaagt tcttcttttt tcctgtcaga
     2341 tgctcttcca tctgtgataa cagaccactt gaaataaaca aaatcaccct ttttggcttt
     2401 tctgtccaca ggggccattg aagcgtatct gcctctgatg ttatcgatgg cttttgtcat
     2461 atctgtatcg gtaacatcaa attttggaac ttcaatagca actttggaag ggtctgcaag
     2521 ctcataatcc gggataaaaa agatttttat tgtaagattg agtgggccat caccttcaaa
     2581 agaatcaact tcaaaagttg gacttccaac aactctgatt gtttttcctg aaagcatttc
     2641 tttgagagct tgccttgcag caatctccgt gaaatcttct ttcattgact caatgccaac
     2701 gtgcgtggcg aaaatcttaa aaggggcttt gccaggtctg aatccgggga tctttatcct
     2761 tgagacatta tctttgtata cctgttcaaa cccttgactc acctcatcag gggtgaaggt
     2821 ggcctttcca actagagttg tccggccctc tggatttgtt gttatttgca tgttcaccta
     2881 aaactttgga gcggaagacg ggacttgaac ccgcgacatc caccttggca aggtggcgct
     2941 ctaccgctga gctacttccg cgccgctatt ttttggtgcg aagggcggga cttgaacccg
     3001 cacaggttta accctactgg atcctaagtc cagcgcgtct gccattccgc caccctcgcg
     3061 cacgaacctt actttcaaac tacgagtttg gtagtatagc aacaaaagtt aacgaatcaa
     3121 ggggtgcttg atgcaacttt ttcggaaaga gaggaccagg cgtcatcgct ggcaagcaag
     3181 attgcatctg gtttgaagaa ggcctgaacc aatccaagat tactaaaaat ccttatttcc
     3241 tcgcccttga acatggggac tgttgttctt attgttttta cttctttttt aataaaaccg
     3301 gacaatgacg aatggacaag ttttgccatt atttcggcag atttgggaat tggcatgcaa
     3361 ccgagtgcaa gaatttcccc atactctcct ctatcaatcg caagaactgc accagcaagt
     3421 ctgttgcccc tcaatgccat aaatgaaatt gagccatcaa ccttgccacc agaggtctct
     3481 ttttgctcta gaagccacct atatcgt
//
LOCUS       DAOSRI010000187         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24219_length_3490_cov_3.860262, whole genome shotgun sequence.
ACCESSION   DAOSRI010000187 DAOSRI010000000
VERSION     DAOSRI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24219_length_3490_cov_3.860262"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK717_06125"
     CDS             <1..399
                     /locus_tag="PK717_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOR47270.1"
                     /translation="FLSSIWPGPVTCVFLRQPKSMMLTTPLKTQPPTVAVRIPDNLVC
                     KMLVGLLGHPLLQTSVNISGLPHGSHNELLYYYERFADAMIDGGPEKESLPSTVINLS
                     GPDAKLVREGSVSWESLKSKLLEFGIGAID"
     gene            473..934
                     /gene="rpiB"
                     /locus_tag="PK717_06130"
     CDS             473..934
                     /gene="rpiB"
                     /locus_tag="PK717_06130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOR47271.1"
                     /translation="MEHPIIAIGSDHAGWQLKELLKPILTQSGYHVIDCGPNSKDSVD
                     YPDYAQLVGQEVSIGHADLGILVCGTGTGMAIAANKIRGIRAANCTNTTQSKLSREHN
                     DANILCLGSRIVGDELAIDIVKSFLQATFLGDRHARRVNKIKSLEEADDRA"
     gene            921..2171
                     /gene="glyA"
                     /locus_tag="PK717_06135"
     CDS             921..2171
                     /gene="glyA"
                     /locus_tag="PK717_06135"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR47272.1"
                     /translation="MTELNRLDPDVAKHLELELDRQRENIELIASENFVSKAVLEALG
                     SIATNKYAEGYPGKRYYGGCEFLDVVEQLAIDRAKELFASDMANVQPHSGAQANLSAY
                     FSILEPGDTIMGMNLSHGGHLTHGHPLSISGRYFKVVPYGVNESTETIDYDEMEKIAS
                     EAKPKVIVAGASAYPRFIDFERISRIAKSVGAYLMVDMAHIAGLVATNLHPSPVPHAD
                     IVTSTTHKTLRGPRGGLIVAKKELSKEINKAVFPGIQGGPLMHVIAAKAVCFKEAAQP
                     EFKTYQEQVLKNAKTLARSLQGMGLRIVSGGTDNHLMLVDLRSKGLSGAEAESLMESI
                     HITINKNSIPFDTAPPLKPSGIRLGTPAVTTRGMKEPEMEQIANIIGRVLTHKDDTKV
                     IEQAKTEVSELTKRFPLYQGLSYL"
     gene            2171..3118
                     /locus_tag="PK717_06140"
     CDS             2171..3118
                     /locus_tag="PK717_06140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOR47273.1"
                     /translation="MSKPLLAFLISLGLAVILTPLAMIFGRKVGITDKVGDTTPSGQT
                     KITKKPTARTGGLALIFAILLPFLFLNLPSSKAIGITGGAVIIFIVMFLDDIYGLGPI
                     PKLLGQLAAATAAIFGGLRIGYLTNIFGQGWLNLEWLSIPLTYFWILTVTNAMNFIDG
                     LDGLAAGITVIATIASAFSSSSRGIFPPTILLASIAGGALGFLPFNFKPAKIFMGDSG
                     AHLIGFLIATATIWGTSKATSAVVIGVAILCLGVPMFDVFFSAIRRILSGKSPLKGDM
                     DNIHYILVKKGWSETSVVLVFYLITAILSAIALSFTALR"
     gene            3126..>3490
                     /locus_tag="PK717_06145"
     CDS             3126..>3490
                     /locus_tag="PK717_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOR47274.1"
                     /translation="MKVLTIFGTRPEAIKMAPLVKLLKNTNGFEAKVVVTAQHREMLD
                     QVLNLFEITPDYDLNIMKKTQSLTEITTKALIGLEEILGKELPDMVLVHGDTTTTFVG
                     ALAAFYQRPSIPCGHVEAGL"
BASE COUNT          997 a          852 c          806 g          835 t
ORIGIN      
        1 ttcctgtcat caatctggcc tgggcctgtg acatgcgtgt tcctgaggca gccaaaaagc
       61 atgatgctca ctacgccact caaaacacaa ccaccgactg tggcagtaag gatacccgac
      121 aacctcgtat gcaaaatgct cgtgggactt cttgggcacc ctttgcttca aacctctgtc
      181 aacatctcgg gtcttccaca cgggtcacac aatgagttgt tgtattacta tgaacgcttt
      241 gctgatgcga tgattgatgg cgggcctgag aaagaatctt tgccatccac ggtaatcaac
      301 ctgtccgggc cagatgcaaa actggtgcgt gagggttctg tttcatggga atcgcttaaa
      361 tcaaagctgt tggagtttgg gataggagca atagactaac ctttaggaat ttcttcagca
      421 acacgcctgc atgccttgca tattgcgctg acgggactat catttttcta gtatggaaca
      481 cccaataata gctatcggca gcgaccatgc cggctggcag ctcaaggagt tgttaaagcc
      541 gatattgacc cagtcaggat accacgtgat tgattgcggt ccaaattcca aagattccgt
      601 agactaccca gattatgccc agcttgttgg ccaagaagta agtatcggcc atgccgatct
      661 tggtattctg gtttgcggga caggaaccgg aatggccata gctgccaaca aaataagggg
      721 cataagggca gccaactgca caaacacaac ccagtcaaag ctatccagag aacacaatga
      781 tgcgaacatt ctctgcttgg gttcaagaat agtcggagat gaactggcaa tcgacatagt
      841 aaaatcattc ctccaggcaa cattccttgg tgataggcat gccaggaggg taaacaaaat
      901 aaaatcattg gaggaagcag atgacagagc ttaataggct ggatccagat gtagcaaaac
      961 atcttgaatt ggaacttgac aggcaaagag aaaacatcga actcattgca tcagaaaact
     1021 tcgtttccaa ggcagtgctt gaggcactgg gttcgattgc aacaaacaaa tatgctgagg
     1081 gctacccggg aaaaagatac tatggtggct gcgagttcct tgatgtagtg gagcagctgg
     1141 ccatcgacag ggcaaaggaa ctttttgcgt ccgatatggc aaacgtccag ccacactccg
     1201 gggcccaggc aaacctttcc gcctatttct ccatcctcga acctggtgac acaatcatgg
     1261 gaatgaacct ttcccacgga ggacacctca cccacggtca cccgctttcg atttcgggaa
     1321 ggtatttcaa ggtagtcccg tatggggtca atgaatccac agaaacaatc gactacgatg
     1381 aaatggagaa gattgcaagt gaagcaaaac caaaggtaat agttgctggt gcctcggctt
     1441 acccacgttt catcgatttc gagagaattt ccagaattgc aaaatcagtc ggcgcatacc
     1501 tcatggtcga tatggcacac atcgctggcc tggtggcaac aaatctccac ccatcaccag
     1561 tcccacatgc cgacattgtc acatcgacaa cccacaagac cctgagaggg ccaaggggtg
     1621 gtcttattgt tgcaaaaaaa gaactttcaa aggaaatcaa caaggctgtg ttccctggaa
     1681 tccagggcgg gccactcatg cacgtcattg ccgcaaaagc tgtatgtttc aaagaagctg
     1741 cacaaccaga attcaagaca tatcaggaac aagtcctcaa aaacgcaaaa acactggcca
     1801 gatcactcca gggaatgggc ctgaggatcg tttctggtgg aacagacaac cacctgatgc
     1861 tagttgatct gaggagcaaa ggtctctctg gtgccgaggc cgaatcactc atggaatcca
     1921 tccacataac aatcaacaag aactcgatac catttgatac tgcaccacct ctcaagccat
     1981 caggcatcag gcttggcacc ccggccgtca caacaagggg catgaaggaa ccagagatgg
     2041 agcagatagc aaatatcatc ggaagggtcc tgacacacaa ggacgacaca aaagtaatcg
     2101 agcaggcaaa aacggaagtt tctgagctga ctaaacgctt cccgctctac caggggcttt
     2161 cttaccttta atgtccaaac cattgctggc tttcctcata tcgcttgggc ttgcggtcat
     2221 cttgacccca ctggcgatga tatttgggag gaaggttggc ataacagaca aggtaggaga
     2281 tacaaccccc agtgggcaaa ccaagataac aaaaaaaccg accgctcgta cgggtggtct
     2341 ggcacttatt ttcgcaatat tactaccatt cctgtttcta aatctcccat catcaaaagc
     2401 cattggcatt actggtgggg cagtaattat tttcatagta atgtttcttg atgatatcta
     2461 cgggcttgga ccaatcccaa aactccttgg gcaactggct gctgcaactg ctgccatctt
     2521 cgggggattg agaattggat atctgacaaa tatcttcggt cagggatggc taaaccttga
     2581 atggctctcg ataccactca cctatttctg gatactgacc gtcacaaacg ccatgaattt
     2641 tattgacgga cttgatggtc tggcagctgg gataaccgtc attgcaacaa tagcatcggc
     2701 tttctcttca agctcaaggg ggatatttcc accaacaatc cttcttgcat caattgctgg
     2761 cggagcactc gggtttcttc ctttcaattt taagccggca aaaatcttca tgggtgattc
     2821 tggtgcccat cttatagggt ttcttatagc aacagctacc atctggggaa catcaaaggc
     2881 tacatctgca gtcgtcattg gcgtagcaat actctgcctt ggtgtcccaa tgtttgatgt
     2941 cttcttttct gccataagaa gaattctaag tggaaaatct ccactgaagg gcgatatgga
     3001 caatatccac tacatcctcg tcaaaaaagg gtggagtgaa acttccgtcg tcttggtttt
     3061 ttaccttatc acagcgatat tatctgcaat cgcactctca ttcacagctc tgaggtgaca
     3121 cacaaatgaa agtcctaaca atttttggaa caagaccaga ggccatcaag atggccccgc
     3181 tggtaaaatt gctcaaaaat actaacggct ttgaggccaa ggtggtcgtt actgcacagc
     3241 acagggaaat gctcgatcag gtgttaaatt tatttgagat aacgcctgac tatgacctca
     3301 acatcatgaa aaaaacacaa agcctgaccg agataacaac caaggctctg attggtctcg
     3361 aggaaatact tggaaaagag ctcccagaca tggtccttgt tcatggtgac acaacaacaa
     3421 cttttgtggg tgcgctggca gctttctatc aaaggccttc catcccctgt ggacacgttg
     3481 aggctggtct
//
LOCUS       DAOSRI010000188         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24410_length_3468_cov_2.843246, whole genome shotgun sequence.
ACCESSION   DAOSRI010000188 DAOSRI010000000
VERSION     DAOSRI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24410_length_3468_cov_2.843246"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="PK717_06150"
     CDS             <1..1988
                     /locus_tag="PK717_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOR47275.1"
                     /translation="IVRIYSPPVENLEAGDIVRGSGNVANPAHATNPGQFDFSNYLLR
                     KDVFCVIFTETPLKIEGKGSLSLDSIGAKIRANAFETFSEALPKRYATTMDSMVFGKS
                     DMPKEILDIFQRTGTSHILAASGFNLTILVFASIWLLLWITGSRKTAIVASIAVAILY
                     AASAGFTPSISRALVMSVLSLSAVLLGRKYTAGSGLAFAVILLLAIDPKTIYDPGFQL
                     SFVACLGFFVMTSSFENIIPKKRWYSGIAIMAIQTIFVQMATLPVLAYHFHTFSTVSV
                     ASNMVAITSSEIIMPLGALSGFLGMIFRPLGVLLCYLAWPILALQNMVLGFLASPEWA
                     TISIGSYHVLVWLAYYTSFAGLCFFICRPKPFIHDIRVKKAFLTTMTISIVLFGGSLV
                     GYAKDGQAQVTFLDVGHGDSCLIRTSSGKTILVDGGGLGGNSSYDIGERVVVPFLRYL
                     GINRIDYVVASHNDIDHLGGLVPVMEQFRVDNIIGSKVPSGTYKEQDFWDLANSKGAQ
                     KIFPFPGQKLDIDPKTSVIFLGPPAEKLGKDSKQINNASVVFKINIDGVEFLFSGDLQ
                     EEGMAFEMKNSQWLDADIIKLPHHGGKNTNLKSWLDAVSPKLAINSNSANQGNGAHKE
                     TAKVLDEMKIPVLSTAENGAITLTVENGKYSILAFKK"
     gene            complement(1978..2358)
                     /locus_tag="PK717_06155"
     CDS             complement(1978..2358)
                     /locus_tag="PK717_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004403788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HOR47276.1"
                     /translation="MKTYFILYVASQERSTEFYKKVLASEPVLFEPGMTEFALSDSCL
                     LGIMPAVDIKRILGSLLPEPSIKHPSSEIYMMVDDPLEYHKRALNAGAVELSPLRERG
                     WGDIAAYSLDLDGNVLVFAKRITS"
     gene            complement(2376..3329)
                     /locus_tag="PK717_06160"
     CDS             complement(2376..3329)
                     /locus_tag="PK717_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR47277.1"
                     /translation="MEPKATRFGYSEALLMLGEKDDRIVALDADLAKSTSSARFRDKF
                     PDRFFNMGIAEQNMVDVAAGLALSGKIPFASSYGVFVTGRAWEQMRTMVCYSKLPVRI
                     AGHAGVSVGADGATHQALEDIAIMNVLPNMHVFSPCDYYETIKATMAAAYVDGPAYIR
                     FGRAPIPIITEVDSPFEVGKATVMKKGSDATVVATGTMVNEALIASADLANFGVSVEV
                     INLSTIKPLDGETILNSVAKTGCIVTAEEHQITGGIGSIIASLLSLNLPVPLEIVGMG
                     DRFGESGEPFELLKHFHCNSCEIVQACLRAISRRQMSRVVL"
BASE COUNT          944 a          849 c          764 g          911 t
ORIGIN      
        1 acatagtaag gatttattcg ccgcctgtag aaaatctgga agcaggagac atcgtaagag
       61 gtagcggcaa cgtggcaaac ccggcccatg caacaaaccc cggccagttt gatttctcaa
      121 actatctttt aagaaaagat gttttttgcg tcattttcac agaaactccc ctgaaaattg
      181 aagggaaggg aagtttgtca ctagacagta tcggcgcaaa aatcagggca aatgcgtttg
      241 agacattctc cgaagctctg ccaaaaagat atgctacgac aatggacagt atggtctttg
      301 gaaaatccga catgccaaaa gaaatactgg acatattcca gaggacgggt acatcacaca
      361 tcctggccgc atcaggattc aaccttacca tactcgtttt tgcctcaatc tggctgctac
      421 tctggataac tggttcaaga aaaacagcca tagtggcttc aattgctgta gccatactct
      481 acgccgcaag tgccggcttc acaccatcaa tatcaagggc gctagtcatg tccgttcttt
      541 cgttgtctgc ggtattgctg ggcaggaaat acactgccgg ttcaggactg gcgtttgccg
      601 taatactgct attggcaata gatccaaaaa caatctatga tccaggattc cagctttctt
      661 ttgttgcatg ccttgggttc tttgtgatga caagctcctt tgaaaacata atcccgaaga
      721 aaagatggta ctccggtatc gccataatgg ccatccagac tattttcgtt cagatggcga
      781 cactgccagt actggcatac cacttccaca cattctcgac agtttcggtc gcttcaaaca
      841 tggtggccat aacaagctcg gaaataataa tgccacttgg cgcactctcc ggtttcctgg
      901 gaatgatttt caggcccctt ggtgttctgc tgtgctacct tgcatggccc attctggccc
      961 tccaaaacat ggttcttggt ttccttgcct ctccggaatg ggccacaatc tccattggct
     1021 cctatcatgt tctcgtctgg cttgcatatt acacctcatt tgctggcctc tgttttttca
     1081 tctgccggcc aaaacctttt attcatgata taagggtaaa aaaagctttt ttaacaacaa
     1141 tgactatctc tattgttctc tttggcggat cactggttgg atatgcaaaa gatggccagg
     1201 ctcaggttac ttttctggat gtcggacatg gagacagctg tctaatacga acatcctccg
     1261 gaaagacaat acttgtcgat ggaggcggac ttggaggtaa ctcttcctat gatatcggag
     1321 agagggtcgt ggtcccattt ttgaggtatc tgggaatcaa caggattgac tatgttgttg
     1381 catcacacaa cgatattgac catctgggcg ggcttgttcc tgtaatggaa cagttcaggg
     1441 tagacaatat tatcggatca aaagtgcctt ccggaacata caaggaacaa gatttctggg
     1501 acctggcaaa ttcaaaaggg gcacaaaaaa tctttccttt ccccgggcaa aaactagaca
     1561 ttgatccaaa aacatcggtc atttttctag gccctccagc tgaaaaactt ggcaaagact
     1621 caaaacagat aaacaatgca agcgtcgtat tcaaaataaa catcgatgga gtagaatttc
     1681 ttttttctgg tgacttgcag gaagaaggaa tggcattcga gatgaaaaac agccagtggc
     1741 ttgatgccga tatcatcaaa ctgccgcacc acggaggaaa aaacaccaac ctcaaatcgt
     1801 ggcttgatgc cgtttcacca aagctggcca tcaactccaa ttcggcaaac caggggaatg
     1861 gcgctcacaa agaaacggca aaggtgctcg atgaaatgaa aatccccgtt ctctcgactg
     1921 cagagaacgg ggccataaca ctcaccgtgg aaaacgggaa atactctatt cttgctttta
     1981 agaagtgatt ctttttgcaa aaaccagaac attgccgtcc aaatccaggc tgtaagcagc
     2041 aatgtctccc caaccgcgct ccctcaaagg gcttagctcg actgcaccag cattcaaggc
     2101 ccttttgtgg tattcaagtg gatcgtcaac catcatgtaa atttcactac ttgggtgctt
     2161 aatggaaggt tcagggagca acgaccccaa tattctctta atgtcaacgg ccggcattat
     2221 gcccagcagg caactgtccg atagtgcaaa ttctgtcatt ccaggctcaa aaaggaccgg
     2281 ctcgctggca agaacttttt tgtaaaactc agtgcttctt tcctggcttg ccacataaag
     2341 tatgaaatat gttttcaatg ccaagccccg gcagactaca gaaccactct cgacatctgt
     2401 cttctggaaa tggccctgag gcatgcctgg acgatctcgc aggaattgca gtggaaatgc
     2461 ttgaggagct caaatggctc gccggattcc ccaaatctgt cacccatgcc aactatctca
     2521 agaggaacag gcaaattgag tgaaaggagg ctggctatta tcgagccgat tccacctgtg
     2581 atctggtgct cttctgccgt tacaatgcaa cctgtttttg caacagaatt gagtatcgtt
     2641 tcgccatcaa gcggcttgat tgtcgagaga tttatcactt caactgatac gccaaaattt
     2701 gcaaggtcgg cgctggctat aagggcttcg ttcaccattg ttccggttgc aacaacagtg
     2761 gcatctgagc ccttcttcat gacggttgct ttgccaactt caaacggcga atcaacctcg
     2821 gttatgatcg gtatcggtgc ccttccaaac cttatgtaag cggggccatc aacataggca
     2881 gcggccatcg ttgcttttat cgtttcataa taatcacaag gcgaaaacac atgcatgttt
     2941 ggaagaacat tcattattgc gatatcttca agtgcctggt gggttgctcc gtccgctcca
     3001 actgaaacac ctgcatgacc tgcaatcctg acggggagct tggaataaca aaccattgtc
     3061 ctcatctgtt cccatgccct gccagtcaca aaaacgccat aacttgaagc aaagggtatt
     3121 ttaccggata gtgcgagtcc tgccgcaaca tcaaccatgt tctgctcggc tatgcccatg
     3181 ttgaaaaatc tgtcgggaaa tttgtccctg aacctcgcgc tggatgttga ttttgcaagg
     3241 tccgcatcaa gtgcaactat acggtcatct ttttcaccaa gcatcaggag cgcttcacta
     3301 taaccaaagc gggttgcctt aggttccatt tctcctccta gtcaactgtt attgtcttgg
     3361 agcagttcct cgggaaatct ggactgtatc ctttaactac agtcaaatga taagcgaaca
     3421 gttgcataaa gacaaccgac agtatcggga caagcgcata ctctgaat
//
LOCUS       DAOSRI010000189         3453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24537_length_3453_cov_3.444673, whole genome shotgun sequence.
ACCESSION   DAOSRI010000189 DAOSRI010000000
VERSION     DAOSRI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24537_length_3453_cov_3.444673"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK717_06165"
     CDS             complement(<1..367)
                     /locus_tag="PK717_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOR47278.1"
                     /translation="MPSLKDNLKRLISRPVRVLVEAALSFNDDNSFIHASSVSFYFIL
                     SIIPFVLFAISFAGNILKHDPALLEAVYKGLSLFIARTEALEWLKNFVDTIIKSSGTT
                     TITGVISLLFVSVGMFRSLE"
     gene            complement(379..453)
                     /locus_tag="PK717_06170"
     tRNA            complement(379..453)
                     /locus_tag="PK717_06170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(419..421),aa:Val,seq:gac)
     gene            547..>3453
                     /locus_tag="PK717_06175"
     CDS             547..>3453
                     /locus_tag="PK717_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47279.1"
                     /translation="MRRVYTLLLIITMIAFPVYGRSQEEKTPDWTHMHGDAFHTAYSS
                     VELPPPLKLLWRKPFDSAPHSNIVACWDSDTPLLFTGDGSGNFFCMKPDTGEVVWKYS
                     VDGSAFLGSPVIFTSYDGTKKVMAVSSDGIKDSGTGSIIAHCFDLKGKIIWKTSLDGT
                     FCESSPTFQNGFLYVATGTMSHGSSSGGYLYKIRQDTGDSVWKASLPDAPATASPSLI
                     GGKVYIACSKLHVGSFDGRWLGAYIRGAVFVAVDDSNGTAVDKLDYGNMRMDILTVYD
                     GEEIILSGQSVAVYRRKYVDPRDPRRFYYVEVEEPKSLVARVKPDNLSTEWRSYPTIY
                     GDGHIYAHTPIVSNGWIIIGADEGVVYAYNKKDATQQWAYKMSNGIRLCMAASDNYIY
                     LNEGDTPPNCGSCKAKFKIVEINSGRILWSYQLDEAGLGGVTVFDKYVYTFDRGNVYC
                     FTRSGPPKLMVDPIKIDLGEIPKGRTEKTSFNVWNGGEGTLIGTVKFNVPYMKLSSDQ
                     FMLTTETKQFVCTIDTKNLTVGQVYNIPILVEATNGDKQTVIITFTVTGLPIIKVTPP
                     TIDFGTVEKGTYSEAEIFIDNIGEGTLRGNIKTDMAWLSLEYQSWEGNHKKLIATADT
                     LMLDYNRSYKANVFFESNGGTAKVEVTIKIKQKGPRLVVYPTELIFTDVNWGDQLTGT
                     FAISNGGIQTLEGTLEPNSDWIILSSKDFSLTTDPKNFKVEIGTKQLKDGATTKGQIL
                     ITSNGGTKVVDITITIKPRPPQLKVDPPTLFFNQCEPDQIYESKITISNLGSGVLSGT
                     IKPGPGADWLKIKQGVFDISKAPIDIPIIVDTKGMAKNTSYTGKIIVESNGGTDEVGV
                     VVVMSERKRFTVELWIGSYEARINSKPSKLDWPPYINKGATMVPARFISEAFGCIVDF
                     YPKHKAIEEIYISRGEVQVTLYVGKNYCLVGNQKVQLDVPPEIKNKRTFVPIRFI"
BASE COUNT         1117 a          732 c          781 g          823 t
ORIGIN      
        1 attcaaggct cctaaacatt ccaacagaaa cgaaaagtaa ggaaataaca ccagtaatcg
       61 ttgttgtgcc gctacttttt atgattgtat caacaaaatt tttcaaccat tccagtgcct
      121 ctgttctggc aataaaaagc gaaagacctt tgtaaaccgc ttcaagaagc gcagggtcat
      181 gcttcaagat gttacctgca aaggagatgg caaaaagtac aaacggtatt attgaaagga
      241 taaagtagaa agatacagag gaggcatgga taaaagagtt gtcatcatta aatgaaaggg
      301 cggcctctac gaggacacga acaggcctgg atatgagacg tttaaggttg tctttcaggc
      361 tgggcatcta ttttgaaatg gtgggtgata gtggaattga accactgacc tcttccgtgt
      421 cagggaagcg ttctacctct gaactaacca cccactggca gcattattat ggttgtaaat
      481 atctatgtgt caagaatatg aaccgaaaat acttaacaaa tgtaatatta ccaaagaggt
      541 gattaaatgc gcagggtata tacactgtta ctaattatca ccatgattgc atttcctgtt
      601 tacgggcgct cccaggagga gaagacgcct gactggacgc acatgcatgg tgatgctttt
      661 catacagcct attcgtcggt cgagcttcca ccgccattga aactcttgtg gagaaaaccc
      721 tttgactctg cgccgcacag caatattgtt gcatgctggg atagcgacac accactgctt
      781 ttcacaggcg atggctccgg aaatttcttc tgcatgaagc cagacactgg tgaagttgtt
      841 tggaaataca gtgttgatgg cagcgctttt cttggctctc cagtaatttt cacctcttat
      901 gatggcacaa aaaaagtaat ggcagtttcc tctgatggta taaaagacag tggcactgga
      961 tcaataatcg cccattgctt cgatttaaag ggcaaaatca tctggaaaac atcactggac
     1021 ggaacattct gcgaatcatc accaaccttt cagaacggct ttctatatgt agctacagga
     1081 actatgagcc acggcagcag ctctggcggg tatctttaca aaataagaca ggacactggt
     1141 gattctgtct ggaaagccag cctgccagat gcgccagcaa cagcatcgcc atctctcatt
     1201 ggcgggaaag tctatattgc atgctccaaa ctccatgttg gcagttttga tggaagatgg
     1261 ctgggtgcct atatcagggg cgctgtattc gtggcagttg acgattcaaa tggcactgct
     1321 gtcgacaagc tagactacgg caatatgagg atggacatcc tcaccgttta tgatggtgag
     1381 gagataattc tttcaggcca atcagttgca gtttacagaa gaaaatacgt tgacccaaga
     1441 gatccaagaa gattttatta tgtagaggtc gaagaaccaa aaagcctcgt tgcaagagtc
     1501 aagccagaca acctctcaac ggaatggcgc agttacccaa caatatatgg agatggtcac
     1561 atttacgcac acacaccgat cgtctcgaac ggctggataa taatcggtgc agatgaaggg
     1621 gttgtctacg cctacaacaa aaaggacgca acacagcaat gggcatacaa aatgtcaaac
     1681 ggaatcaggc tttgcatggc cgcttccgac aactacatct atctgaatga aggcgataca
     1741 ccgccaaatt gcgggtcttg caaagccaaa ttcaagatag tggagataaa cagtgggaga
     1801 atactctgga gttaccagct tgatgaagct ggtcttggtg gagtcactgt tttcgacaaa
     1861 tatgtttaca cttttgacag agggaacgtc tactgtttta caagatcagg cccgccaaaa
     1921 ttaatggttg atccaataaa aatcgacctt ggtgagatac caaagggcag gaccgaaaaa
     1981 acaagtttca atgtctggaa tggcggtgaa ggaacgctta tcgggactgt aaaattcaat
     2041 gttccataca tgaaactttc ctctgaccaa ttcatgctga caaccgagac aaaacagttt
     2101 gtctgcacga tagacaccaa aaatctcaca gtggggcagg tttacaatat accaatactg
     2161 gttgaggcta caaacggcga taagcagacg gtcataatca ccttcacagt gacaggcctg
     2221 ccaataatca aggtcacacc gccaacaatt gactttggaa cagtcgagaa gggtacttac
     2281 agcgaagccg aaatattcat agacaatatt ggtgaaggta ctcttagagg caatattaaa
     2341 actgacatgg cttggttgtc actggaatac caatcatggg aaggaaacca caaaaaactc
     2401 atcgcaacag ccgacacact gatgctcgat tataatagga gctacaaagc aaacgtgttc
     2461 tttgaaagca acggtggtac tgcgaaagtt gaggtcacga taaaaataaa acagaaaggg
     2521 ccaaggcttg ttgtttatcc aacagaattg atttttacag atgtaaactg gggtgaccag
     2581 ctgacaggta cctttgcaat ctcaaatggc ggcatccaga cacttgaagg gacccttgag
     2641 ccaaacagcg actggataat cctttcatca aaggatttct cactcaccac cgacccaaaa
     2701 aatttcaagg tggaaatagg caccaaacaa cttaaagatg gggcaacaac caagggccaa
     2761 atattgataa caagcaatgg cggtacaaag gtagtagata taaccatcac aataaagccc
     2821 aggccaccac agctcaaggt tgatccacca acactattct tcaaccaatg tgagccagac
     2881 cagatatacg aatcaaaaat aacaatttcc aatttgggtt cgggtgtcct ctctggtaca
     2941 ataaaaccag gcccaggagc cgactggctc aaaataaaac aaggtgtttt tgacataagc
     3001 aaggcaccga ttgatatacc cattattgtc gacacaaaag ggatggcgaa gaacacctcc
     3061 tacacaggca agataattgt cgagtcaaat ggtggaactg atgaagtcgg tgttgttgtc
     3121 gtcatgagcg agagaaaacg ttttacagtc gagctctgga ttggttccta tgaagcaaga
     3181 ataaacagca aaccatcaaa acttgattgg cctccataca tcaacaaagg tgcaacgatg
     3241 gtcccagcaa gatttatctc agaggccttc gggtgcatag ttgattttta cccaaaacat
     3301 aaggccatag aagaaattta catctcgaga ggagaggtgc aggtaacgct ttatgtaggg
     3361 aaaaattatt gcctcgtcgg gaatcagaaa gtgcagcttg atgttccacc agaaataaaa
     3421 aacaagagga cctttgttcc aataagattc ata
//
LOCUS       DAOSRI010000190         3444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24633_length_3444_cov_4.321924, whole genome shotgun sequence.
ACCESSION   DAOSRI010000190 DAOSRI010000000
VERSION     DAOSRI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24633_length_3444_cov_4.321924"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK717_06180"
     CDS             <1..331
                     /locus_tag="PK717_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47280.1"
                     /translation="SIFNCRTDELFEVFGKVKAWLSPKGRFLVTLRSAKTPAPDDSVL
                     LPGESGTYITKRDGFEHFVHYSDCEEVEKLSLGWGLASLDEIEELLGMGDKKRKVWTY
                     RLTLEKQ"
     gene            complement(345..1163)
                     /locus_tag="PK717_06185"
     CDS             complement(345..1163)
                     /locus_tag="PK717_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47281.1"
                     /translation="MAHFQDVFLFTSLIVLTHRKLTGMVKFGFEDGDSARFAFKSGRI
                     IQGPPNGKTDELLFRTSGELSILGYDNPSFSAQKDNGWMPDIEDILEDLPQISATSYP
                     CMSRMFVFPTGIPFRSNKIWANKAELILSRIGNGVSTQYIMNWIDPESFWKAIFRLIN
                     DNIIDFSYDETVGVIANRFLKKFAKVIAQKNGKNIATNFEKRVNTESEMLFNPSSPKE
                     LSPVYGAYPHAYKIRAIAKLAGLINKNDSHQKNISDTLQELSADERQLVSLLLK"
     gene            complement(1261..1707)
                     /locus_tag="PK717_06190"
     CDS             complement(1261..1707)
                     /locus_tag="PK717_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOR47282.1"
                     /translation="MWKRSWQNLKNQDWFKPFDHTADLGACLNADSLEGLVRQGALAL
                     LFFTIDPATVEANQEKSFEVEFDSPEQLLVRTLSKLVYLVDCEGFLTRNMELKISGNS
                     ALVKIYGEIYNKNRHQTKHLIKAVTWGGLEVKVEDGKYLATVIFDD"
     gene            complement(1661..>3444)
                     /locus_tag="PK717_06195"
     CDS             complement(1661..>3444)
                     /locus_tag="PK717_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOR47283.1"
                     /translation="IRKFYEEGRGTIIVQGIGKVESEGKKLSYVINELPYMVNKATLV
                     STIADLVREKKIEGITDLRDESDREGIRVVIELKSGADPQLIEKQLYKHTAYQSTFGG
                     IMLSIVDGRPMEVGLIDMLNTFLDFRRQVVTKRTEFQLKKAEERKHILEGIRTAITNI
                     DETIKIIRNSPTSEAAQTSLIQRFQLSDIQAKAILDMRLARLTALEVDKIDKEIAELI
                     AKIKELTEILENPERLKQVVRDEFLLLKKKFADKRRTVIENFDESGIKLDDLIPDDPF
                     IVSITKDGYLKRVKVDAWRAQGRGGKGVTGVQTVGDDQAQDIFHVSAKQDMLTFTNLG
                     KVYKFKVYDIPETGRAAKGTPITAIIPLTPSEFATYILPVKQWDDNHFLMFVTAQGTV
                     KKVELSSFANIRRTGIIAITLDGDDWLRKARLVNPNEEIIIVSKHGQAVRFNESDIRP
                     TGRSSKGVIGIRMGIGDMVMGMEVEEEDGCLLCVSENGYGKLTDFDTFRLIKRGGKGV
                     RAMNINDKSGPLVACNIVKPGELLMIITTSGQTIRISTSDIPELGRYATGVRLIKLEQ
                     GDSVTAISRMTDNVEEELAEPEEPGLV"
BASE COUNT          757 a          794 c          816 g         1077 t
ORIGIN      
        1 ttccattttc aattgccgga cagacgagct ttttgaggtt tttggaaagg tgaaagcctg
       61 gctttctcca aaaggcagat ttcttgttac gttgaggagc gcaaaaaccc cggccccgga
      121 tgattctgtt ttgctccccg gggagagtgg aacatatata actaaaagag atgggtttga
      181 gcattttgtc cactacagcg attgcgaaga agtcgaaaag ctttctttgg gctggggcct
      241 ggcttcactc gatgagatag aagaacttct ggggatggga gacaagaagc gcaaagtctg
      301 gacctacaga ttgacattgg aaaagcaata agtctgccat gattttattt aagtagcaga
      361 cttacgagtt gcctttcgtc tgcagagagt tcctggagtg tatctgaaat gtttttttga
      421 tgggaatcgt ttttgtttat aagccctgcg agtttcgcta tggcccttat tttgtaggcg
      481 tgcgggtatg ctccgtacac cggcgacagc tcttttggcg atgatggatt gaagagcatt
      541 tctgattctg tattcactct tttttcaaag tttgttgcaa tgtttttgcc attcttttgg
      601 gcaatcacct tggcgaattt tttaaggaag cggtttgcaa tgacgccaac ggtttcgtca
      661 taggaaaaat ctataatgtt gtcattgatg agtctgaaaa ttgctttcca gaaagattct
      721 ggatcaatcc agttcattat atattgagtg gatacgccat tgccgatgcg ggaaaggatc
      781 agctccgcct tgtttgccca tattttgttt gacctaaagg gaatacctgt cggaaaaaca
      841 aacattctgc tcatgcacgg gtacgatgtt gcagatattt gtggcaaatc ctccaggata
      901 tcttcaatgt ctggcatcca gccgttgtcc ttctgtgcag agaatgaagg attgtcatag
      961 cctaggattg atagttcgcc agatgtcctg aagaggagct cgtcagtttt tccatttggc
     1021 gggccctgga ttattcggcc cgatttgaat gcaaaccttg cactatcccc atcttcgaag
     1081 ccaaacttga ccataccggt cagttttcta tgggtgagaa caatgagtga ggtgaataaa
     1141 aacacgtctt ggaagtgggc catggcctct tttcaaaaaa atagacgaat tttataaagt
     1201 taagcttatt ttaatgatat ctagtatcaa gtcaaataga caaatcctta tttattcagc
     1261 ctaatcgtcg aatatgactg ttgccaggta ctttccgtct tcgactttca cctcaagccc
     1321 tccccaggta acggccttta tcaggtgctt tgtctggtgc ctgtttttgt tgtagatttc
     1381 accgtaaatt tttaccagag cgctgttccc agatattttg agttccatgt tgcgggtgag
     1441 gaacccctcg caatcaacca gatagaccag cttcgaaagt gttcgaacca gcagctgctc
     1501 cggggaatcg aattcaacct caaagctttt ttcctggttt gcttccacgg ttgccggatc
     1561 tattgtgaaa aaaagaaggg ccagcgcgcc ctggcgcacc agcccttcca gagaatctgc
     1621 attcaaacag gcccctaggt ctgccgtatg gtcaaatggc ttaaaccagt cctggttctt
     1681 caggttctgc cagctcctct tccacattgt ccgtcatcct tgagattgcg gttactgagt
     1741 cgccctgctc gagttttatg agcctgactc ctgttgcgta tcttccaagc tctggtatgt
     1801 cagaggttga aatcctgatt gtttggccgc tcgttgtgat tatcatgagc aattcgccag
     1861 gttttacgat gttgcaggca acaagtgggc cggacttgtc atttatgttc atggcgcgga
     1921 cgccctttcc gccgcgcttt atgagcctga atgtgtcgaa gtcggtgagc ttgccatatc
     1981 cgttctcaga aacacagagc aggcaaccat cttcttcttc aacttccatg cccatgacca
     2041 tgtcaccaat gcccatcctg attccgataa caccctttga tgacctgccg gttggcctga
     2101 tgtcgctttc attaaagcgt acggcctggc catgcttgga gacaattatt atctcttcat
     2161 tagggttgac cagccttgct ttcctgagcc agtcatcgcc atccagcgtt attgctatga
     2221 ttccagttct tctgatgttt gcaaaagagg atagttcaac ttttttgaca gtgccctgtg
     2281 ccgtcacaaa catcaggaag tgattgtcgt cccattgttt gactggaagt atgtaggtag
     2341 caaactccga tggtgtaaga ggaatgattg ctgttatcgg ggtgcccttt gccgcccttc
     2401 cggtttccgg gatatcgtat accttgaatt tgtaaacttt tccgaggttt gtgaatgtca
     2461 gcatgtcctg tttggctgaa acgtggaaga tgtcctgggc ctggtcgtcg ccgactgttt
     2521 gcacgccagt gactcccttt ccacccctgc cctgggccct ccaggcatcg acctttactc
     2581 tcttgagata cccgtctttt gtgattgaaa caatgaatgg gtcgtctgga atgaggtcgt
     2641 cgagcttgat gccggactca tcgaagtttt caataactgt gcgtctttta tctgcaaact
     2701 tctttttgag gagcaggaac tcatccctga cgacctgttt gagtctttcg gggttctcga
     2761 ggatttcggt gagttctttg atttttgcta taagttctgc tatttccttg tctatcttgt
     2821 caacttccaa ggcggtgagc cttgccagtc tcatatcgag gattgctttt gcctgtatgt
     2881 cggacaattg aaatctttgg attagggacg tctgggcagc ctccgaggtt ggcgaattcc
     2941 tgattatctt gatggtttca tcaatattgg tgatagctgt tctgatgcct tctaagatat
     3001 gctttctttc ttcggctttt ttgagctgga actcggtgcg cttcgtcaca acctggcgcc
     3061 tgaaatcaag gaatgtattg agcatgtcaa tcagtccaac ttccatcggc ctgccgtcca
     3121 caatggagag cattattccg ccaaatgttg actggtatgc ggtgtgtttg taaagctgct
     3181 tttctatgag ctggggatca gcgccagatt tgagctcgat gactaccctg atgccttccc
     3241 tgtcagattc gtccctcagg tcggttatgc cttcgatctt cttttccctg acgaggtcgg
     3301 ctattgtgct aacgagtgtg gccttgttga ccatgtatgg cagttcattg atcacatagc
     3361 tcagcttttt gccttcggac tccaccttgc caatgccctg gacgattatc gtacctctgc
     3421 cctcttcgta gaacttcctt attc
//
LOCUS       DAOSRI010000191         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24896_length_3417_cov_3.658537, whole genome shotgun sequence.
ACCESSION   DAOSRI010000191 DAOSRI010000000
VERSION     DAOSRI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24896_length_3417_cov_3.658537"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..921)
                     /locus_tag="PK717_06200"
     CDS             complement(133..921)
                     /locus_tag="PK717_06200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOR47284.1"
                     /translation="MARTRLKTRKKTKSPKKKETCSILTDQVKTRLNRYIASSGLCSR
                     RNADQLILAGRVFVDGTLETNPGTMVTLQNIVKVDGKIIKPVEEKVYIALNKPKLVIT
                     SKSDEQGRNTVMDFLPEKFLHLNPVGRLDFDSSGLVIMTNDGELANFLTHPKFSFPKT
                     YQVRANPPFEREDVDAILSGVQIAEDVWVRADKAILSGEVLTLTIHEGKYHHIKRMAE
                     SLGKKVCFLRRVAIGPVKIDGLLSGQWRMLTDKELLALLEKTGR"
     gene            complement(845..2095)
                     /locus_tag="PK717_06205"
     CDS             complement(845..2095)
                     /locus_tag="PK717_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOR47285.1"
                     /translation="MSEINGLLSEAFSKIQYCDKILVASSDGLIITAYPDYNPDKNDS
                     LAASSTSVLGLTAATSELAGKGDAEYVLIRSNNGFMMTLGQQNFSVLVMAGQKANLGM
                     LLLQGQKLVEKLSGIIDEHIAPKKLEHPRIYARELGTIETESVEEIHPEEVSYTSTKS
                     RDSQPESESPEQGIGIPAFSKPFSDYSKEPGDSFLAGSQGYEEEPIAIEPNFKPKMED
                     VQDVLQQPVEEITCHEECNECEECEEPDESEHFLTEPEEIKAKFWPEEPTRIDEHPEH
                     TPDIEIEEKSSESIYSDRDLQAPEENPTEPMEEPIIEPTEENPEETHENMTTSPIVDL
                     VLPKGIYINDVVLYSNKQGLENLVVHKEEEQSTQQEDQQQEEEQKPPEAMPWFNFNNN
                     GENKTQNPEEDEKPQKEGDLFDSY"
     gene            complement(2092..2508)
                     /locus_tag="PK717_06210"
     CDS             complement(2092..2508)
                     /locus_tag="PK717_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47286.1"
                     /translation="MKKESHKMCDITNMKEDKVELDTILEGLKDELPEFVAAAVAGSD
                     GLTISTLAKDSSVDMSMVGAEFASIVSMVSKAGREINAGKLHSSIFSTENYHILTSQI
                     GETPFFQIVCLKSDGNIGKARYLMGKVEARIKEAIL"
     gene            2573..>3417
                     /locus_tag="PK717_06215"
     CDS             2573..>3417
                     /locus_tag="PK717_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR47287.1"
                     /translation="MSSIASTFVSSGFARSILRASYPLSKLAIDSIGGRRKPKGLQKA
                     YVWAFSAIMRSYMASLGVSWSEVKKTLSDPGIARCFDLVVKSVIEYGLKKPIVLSAPF
                     SVVYNLTNKCNLRCVHCFQSADSSLGDLMTKEQKFSIIKQMADLGTAAITFSGGEPLL
                     SPDFWDCVRYATENRIYCSIDTNGTLIDDSVAEKLFKAGLRSAQVSIDSPFPEKHDEF
                     RCMKGAFDLSMRGVKAMKKAGLFVSMGVTLTSNNIGLIDDFVALAKKHSFNRIVFYHL
                     IPTGDL"
BASE COUNT          799 a          794 c          746 g         1078 t
ORIGIN      
        1 cgtgcatgtc tcctatatga taggtgtttt aaaaccagac gatggtgtga accatcgaaa
       61 aaccattaaa gcgaataaag cggggatgtc aagactctct ctttttaagg gagtctttct
      121 gggcgttcca aattatctcc ctgttttttc cagcaacgct aaaagttctt tatctgttaa
      181 cattctccat tgtccggaca ataatccatc aatctttacg ggaccaatgg ctactctccg
      241 gaggaaacat acttttttgc caagtgattc tgccattctt ttgatatgat ggtacttgcc
      301 ctcgtggatg gtcaatgtga gcacctcgcc ggacaggatt gccttgtcgg ccctcaccca
      361 aacgtcttct gcgatctgca caccactaag aattgcatca acgtcttccc tttcgaaggg
      421 agggtttgcc ctgacctgat aggtttttgg aaagctgaat ttgggatggg tcaggaaatt
      481 ggccagttcg ccatcgtttg tcattattac gaggccgctt gaatcaaaat caagccttcc
      541 gacaggatta aggtgaagga atttttccgg aagaaagtcc attacagtat ttcttccctg
      601 ctcgtcactt ttgcttgtga tcacaagctt tggtttgttc agggcaatgt agaccttttc
      661 ctcgactggc tttatgattt ttccatcaac ctttacaata ttttgtaaag tgaccattgt
      721 tccagggttt gtttcaagag tcccgtcaac aaaaacccga ccagccaata tgagctggtc
      781 ggcatttctt ctcgaacaca ggcccgaaga cgcaatatac ctgttcagtc ttgttttgac
      841 ttgatcagta agaatcgaac aggtctcctt ctttttgggg cttttcgtct tcttccgggt
      901 tttgagtctt gttctcgcca ttattattaa agttaaacca tggcatggct tcaggaggtt
      961 tttgttcttc ttcctgttgt tgatcttctt gttgtgtgga ctgttcctct tctttatgga
     1021 caaccaggtt ttccagcccc tgtttattcg agtataagac tacatcgttt atgtaaatgc
     1081 ctttaggcag aacgaggtca acgattggag atgttgtcat gttttcgtgc gtttcttctg
     1141 gattctcctc cgttggttct attatcggct cttccattgg ctctgttgga ttttcttctg
     1201 gcgcctggag atcccggtca gaataaatgc tctccgagga tttttcctca atttcaatat
     1261 ctggagtgtg ttctggatgt tcatcaattc tggttggctc ttccggccag aattttgctt
     1321 ttatctcctc gggttctgtc aggaagtgct ccgattcatc cggttcttcg cattcttcgc
     1381 attcgttgca ctcttcatga caggtaattt cctcgacagg ttgttgcaag acatcctgca
     1441 catcttccat ttttggtttg aaattgggtt ctatggcgat gggttcttct tcataaccct
     1501 gtgaaccggc caaaaagctg tcgccaggtt ctttggaata atcagaaaac ggttttgaaa
     1561 aggcaggtat gcctatgccc tgttcgggag attctgattc aggctgtgaa tccctggatt
     1621 ttgtggaagt gtaagaaacc tcttcagggt gtatttcttc aacactctca gtttcaatgg
     1681 tgccaagctc tctggcataa attcttgggt gttcaagttt ttttggtgca atgtgttcat
     1741 caattatacc actgagcttt tcaacaagtt tctgcccctg aagcaggagc atgccgaggt
     1801 ttgctttttg cccggccatt acaagcacag agaaattctg ttgacccagt gtcatcatga
     1861 agccgttgtt tgaccttatg aggacgtact cggcatcacc ttttccagca agctcggatg
     1921 ttgcagcggt aagcccaagg acagatgttg atgatgcagc gagggaatcg tttttgtccg
     1981 gattgtagtc agggtatgcc gttataatga ggccgtctga tgatgctaca agtatcttgt
     2041 cgcaatactg aatctttgaa aaagcctctg acagtagccc gtttatctca ctcatagaat
     2101 tgcttccttg attctagcct caacctttcc cattaaatat cttgcttttc caatgttccc
     2161 atcggatttg aggcagacta tctggaagaa tggagtttca ccaatttgtg aagtcaggat
     2221 gtgataattt tctgtcgaga agattgaact gtgaagcttc cctgcgttta tttctctccc
     2281 tgcctttgaa accatcgaga caatcgaggc aaattcggcc cctaccatac tcatgtcaac
     2341 cgagctatct tttgcgaggg tcgagattgt caggccatca ctacctgcaa cggccgcagc
     2401 aacgaattcg gggagctcgt ccttcaatcc ttcaagaatt gtgtccagtt ccactttgtc
     2461 ctccttcata ttggtgatat cgcacatctt atgcgattct tttttcatag tcaagccccg
     2521 acctcgtttt gtcctttcat tttatcgacg ccctattata attctttgga atatgtcaag
     2581 cattgcatca acatttgttt ctagtggttt tgccaggtct atcctcaggg caagctatcc
     2641 tctttcaaag cttgccatcg acagcattgg cggaagaaga aaaccgaaag gactccagaa
     2701 agcttatgtc tgggctttct cggcgatcat gaggtcctac atggcctccc ttggagtgtc
     2761 atggagtgaa gtaaagaaaa ctctttccga tccagggatt gcacgttgct tcgatcttgt
     2821 tgtaaaaagc gtcatcgaat atggcctcaa aaaaccgatt gttctttctg cgccattttc
     2881 ggtggtctac aatctgacaa acaagtgcaa cttgaggtgc gtccactgct tccagagcgc
     2941 agactcctcg cttggcgacc ttatgacaaa agagcaaaaa ttctccatta tcaagcagat
     3001 ggccgatctt ggaacagcgg ccataacatt ctccggtgga gagccgctcc tctcgccaga
     3061 tttctgggac tgtgtcagat atgccacaga aaacagaatc tactgctcaa tagacacaaa
     3121 tggcaccctt attgatgaca gcgttgctga aaagctcttc aaagcaggtt taaggtctgc
     3181 ccaagtctcg attgattcac cattccctga aaagcacgat gaattcaggt gcatgaaggg
     3241 cgcattcgac ctttccatgc gaggcgtgaa ggcgatgaag aaagcagggc tctttgtttc
     3301 aatgggcgtg accctcacct caaacaatat tgggctcata gatgattttg ttgcgctagc
     3361 aaaaaagcac tctttcaaca ggattgtttt ctaccatctc atacctacag gcgatct
//
LOCUS       DAOSRI010000192         3416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_24904_length_3416_cov_2.888724, whole genome shotgun sequence.
ACCESSION   DAOSRI010000192 DAOSRI010000000
VERSION     DAOSRI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24904_length_3416_cov_2.888724"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK717_06220"
     CDS             <1..528
                     /locus_tag="PK717_06220"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOR47288.1"
                     /translation="TKKGIEMVSMNLLIPDPTTPVIWRGPVLAGVLRQFYTDVNWGNL
                     DLLFVDLPPGTADVPLTVFQIFPIDGMVVVTSPQELANLVVTKALKMAAEMSIPLVGV
                     VENMTYFVCKDGEKVEVFGKSRVQERCKEQGVPYLGGMPIDPSYARIADEGAIEDAVI
                     PQAVEDTLKTILGEK"
     gene            525..758
                     /locus_tag="PK717_06225"
     CDS             525..758
                     /locus_tag="PK717_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOR47289.1"
                     /translation="MTENNKNANIITGDETFGEVIQKYPQVIPVFFQFGLHCIGCHIS
                     AYESIKDGAAAHGVEIEKLIEALNKAINEPKKD"
     gene            808..2985
                     /locus_tag="PK717_06230"
     CDS             808..2985
                     /locus_tag="PK717_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47290.1"
                     /translation="MRKLALAIVVFWVATSPVQAFPLGWPSYRHDHSHSGASDIPLSP
                     PLVQSWSYQVGSELSTEAVSSGPRVYVGSKKLYCFDKDREADNLLWSLDVPSEITTTP
                     FTTFDTVIFASQDGFITAIDTEARSQRWKMQIGKVQRGIGSYKESCIFRNEKGDLLSV
                     DIKTGQVNFKVNILCPISVPPCIADDKIFVCCDAGKLYCLNASNGAFIWKSDVTTSDV
                     IGGMLYHEGAIYFGSYDNRVYAVSASDGKTIWSKKVDGWIGAHPVAVGNEIFVRSKLS
                     TLWCFDSKSGETIWHFPHEPTKTEPVVSGDYIYIGSNRRIVAFSVSKRIEIWYFEFAK
                     EQPTSLSMSGDRLLVGTNGGRIYCLRAGPGFELSSDKIDLEVMTTDERPSFELSVKNN
                     REDRWPSMLVGDVTTDYPWMVLEDKVFKLRTGESKKFIINIKLDELNKVGVHDGKLYV
                     RYLYGVNEALPGEEPETVVIVPVTLKYIDPFPPKICVEKETIDVGAVTLGQTKKANFV
                     IKNCGKGKLVFSLNAQSLEGWLRIVDNQPTFSLMPDETKIVTVEVAAYGFAIEPGDDC
                     QKTGVIEIQSNTEQSVIPLTVTCRIYDMVLPTVVSIPIGSNKASINGKKVDVTPPSYV
                     SGSRTMVPLRIIAEAFFARVSWDPSDRSITITNCTSQSRFTVGSKKVEIIGMGEVKTT
                     TIDSPPEIRSGKTFIPLRAVSDILGASIAWEQKTKTVTITYSP"
     gene            2985..>3416
                     /locus_tag="PK717_06235"
     CDS             2985..>3416
                     /locus_tag="PK717_06235"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOR47291.1"
                     /translation="MTKRIVFRGTVQGVGFRPFVWRVADSFGLKGFVRNIGNGVEAVF
                     CGESSAIQNAIEMIRTSPPPQAIIESVEVADFEGDYKDLKILETPEGAPSSVLVPPDI
                     GICDDCRRELLDPKDPRHLHPFISCTNCGPRLSIIRRLPYDR"
BASE COUNT          990 a          711 c          818 g          897 t
ORIGIN      
        1 accaaaaagg ggatcgagat ggtttcaatg aatcttctca ttcctgaccc aacgactcct
       61 gtaatctgga gaggcccagt cctggctggt gttttgaggc aattttatac agatgtcaat
      121 tggggcaatc ttgacctgct ctttgtagac ctcccgccag gaactgccga tgttccgctg
      181 acagttttcc agatattccc gattgatggg atggttgttg tgacaagccc tcaggaattg
      241 gcgaaccttg ttgtcactaa ggctctcaag atggctgctg aaatgagcat tccgcttgtt
      301 ggtgttgtgg aaaacatgac ctactttgtc tgcaaagatg gcgagaaagt agaagtgttc
      361 gggaaatcca gggtgcagga aagatgcaag gaacagggtg ttccatatct tggtggcatg
      421 ccgatagacc cgtcatacgc aaggattgca gatgagggag caattgaaga tgcagtgata
      481 cctcaagcag ttgaggatac ccttaaaacc atattggggg aaaaatgacc gaaaataata
      541 aaaatgcaaa catcataacc ggagatgaaa cttttggcga agtgatacag aaataccctc
      601 aggtaatacc ggtatttttc cagtttggac tacattgtat cggttgccac ataagcgcct
      661 atgaatctat caaggatggc gctgctgctc acggtgtcga aattgaaaaa ctcattgagg
      721 ccctgaacaa ggcgataaac gagccaaaaa aagactaggg gctatttact tttgccaaaa
      781 aacacatatc tgtaatccca aagagcaatg cgtaaacttg cactcgcaat agttgttttt
      841 tgggtggcaa ccagtcctgt ccaggctttc cctttgggat ggccttcgta cagacatgat
      901 cactcgcatt ccggggcttc tgatatccct ttgtcgcccc cgcttgtcca gtcatggtca
      961 tatcaggtcg gcagtgagct gtccacagag gctgtaagtt ctggtccaag agtttatgtg
     1021 ggctcaaaaa agctctattg ttttgacaaa gacagagagg cagataatct tctgtggtcg
     1081 cttgacgttc catctgaaat aaccactaca ccatttacaa ccttcgacac tgttatcttt
     1141 gcttctcaag atggttttat aacagccatc gacactgaag caagaagcca gagatggaaa
     1201 atgcagatag gtaaggtaca aaggggcatt ggctcctaca aagaatcttg catttttaga
     1261 aacgagaagg gtgacctcct gagtgttgat ataaaaacgg gccaagtaaa tttcaaagta
     1321 aacatattgt gcccaatttc tgttccgccc tgcattgcag atgacaagat ttttgtttgc
     1381 tgtgatgccg ggaaacttta ttgcctgaac gcaagcaatg gtgcttttat ctggaaaagt
     1441 gatgtcacaa catcagatgt gataggcggg atgctctacc atgaaggcgc aatatatttt
     1501 ggcagttatg acaacagggt ttatgcagtt tctgcaagtg atggcaagac aatctggtca
     1561 aaaaaagttg atggctggat aggtgcgcat ccagttgctg taggaaacga gatttttgta
     1621 agatcaaaac tcagcacatt gtggtgcttt gattcaaaaa gtggtgaaac catctggcat
     1681 tttccccatg aaccaactaa gactgagcca gtcgtttctg gtgattacat ttatattgga
     1741 tcaaatcgcc ggatagtcgc tttttctgtc tcaaaaagga tcgaaatctg gtactttgaa
     1801 tttgccaaag aacaacccac ctcactttca atgtcagggg acagacttct ggttggtaca
     1861 aatggtgggc gcatctactg cctaagggcc gggccaggtt ttgaactctc cagtgacaag
     1921 atcgaccttg aggtcatgac cactgacgaa cgcccctcgt ttgaactctc agtaaaaaac
     1981 aatagagaag accgttggcc ttcgatgcta gtcggagatg tcacgaccga ttacccatgg
     2041 atggtgcttg aagacaaagt tttcaagttg aggactggtg aaagcaaaaa gtttataatc
     2101 aacataaaac ttgacgaact caacaaggtt ggcgtccatg acggaaagct ttatgtcagg
     2161 tacctttatg gtgtaaatga ggcccttccg ggagaggaac cagaaacagt ggtcatagtt
     2221 ccagtgactt taaaatatat agacccattc ccacccaaaa tatgtgttga aaaagaaact
     2281 atagatgtcg gagcggtaac gcttggccaa acaaagaagg ccaattttgt aataaaaaat
     2341 tgcggaaagg gaaagctcgt tttttctctg aatgcgcaat cgcttgaggg ttggctgagg
     2401 attgttgaca accagccaac attctccctg atgccggatg agacaaaaat tgttacagtg
     2461 gaagttgccg cttatggctt tgctattgag cctggcgatg actgtcaaaa gacgggggta
     2521 attgaaattc aaagcaacac cgaacaaagt gttattcctc ttactgttac ctgcaggatt
     2581 tatgacatgg ttttgcccac cgttgtttcg attcctattg gttcaaacaa agccagcata
     2641 aacgggaaaa aagttgatgt taccccaccc tcctatgtct ccgggtcaag aacgatggtg
     2701 ccactgcgaa tcattgcaga ggcctttttt gcaagggtat catgggatcc gtctgacaga
     2761 agcatcacaa taacaaattg tacaagccag tcgaggttta ccgttggcag caaaaaagtt
     2821 gagatcattg gaatgggcga ggtcaagaca acgacaatcg attcaccccc cgaaatcagg
     2881 tcgggcaaga cctttatccc tttacgtgcc gtctctgata ttcttggcgc atcgattgcc
     2941 tgggaacaga aaacaaaaac agttacgata acatatagtc cctgatgaca aagaggattg
     3001 ttttcagggg gacagttcaa ggtgttggtt tcaggccatt tgtttggaga gttgctgatt
     3061 cttttggcct caaaggtttt gtcaggaaca ttggcaacgg cgtagaggct gttttttgtg
     3121 gagaatcctc tgcgatccag aatgcaatcg aaatgataag aacttctcca ccgccacaag
     3181 caatcattga atcagtagaa gttgctgatt tcgaagggga ttacaaagac cttaagatcc
     3241 tcgaaacacc agagggtgct ccatcatcgg tgcttgtgcc gcctgacata ggcatttgtg
     3301 acgattgtcg aagggaactt ctggacccaa aagacccaag acatttacat cctttcatat
     3361 cttgcacaaa ttgtgggccg agactttcga taataagaag attgccctat gacaga
//
LOCUS       DAOSRI010000193         3387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_25165_length_3387_cov_3.725390, whole genome shotgun sequence.
ACCESSION   DAOSRI010000193 DAOSRI010000000
VERSION     DAOSRI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25165_length_3387_cov_3.725390"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK717_06240"
     CDS             <1..276
                     /locus_tag="PK717_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47292.1"
                     /translation="SFECQVIWDQPTKTVTIIYNRRGITVYVTIGSKTAFIEYRRGGG
                     KIPILMKDPPVIVGGKTFVPVQFMAQAFDARWVMISASQIKITIKGS"
     gene            complement(263..1033)
                     /locus_tag="PK717_06245"
     CDS             complement(263..1033)
                     /locus_tag="PK717_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR47293.1"
                     /translation="MEIIDTHAHLDLPDFKGEFERVLKRAKEGDVCQIINVGFDLESS
                     SRSVSLAQNNREIFATVGIHPHEAKKWGSEIRSWIIELSGREKVVAIGEIGLDYYRDL
                     SYRQTQREVFSEQIEIAKNLGKPVVIHSRDAQGDVMRMLEEHSPKSVVLHCFSGDTLM
                     AEWAFERGYHLSFGGPLTYPRNERLEMAATIAPEDLIMVETDCPYLSPVPLRGKRNEP
                     SYIRHTLIRLANIRKCSVEKMAEITTANARRFFSITNP"
     gene            complement(1033..2958)
                     /gene="metG"
                     /locus_tag="PK717_06250"
     CDS             complement(1033..2958)
                     /gene="metG"
                     /locus_tag="PK717_06250"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR47294.1"
                     /translation="MKKYYVTTPIYYINDNPHIGHVYTTMAADIIARYRKSMGREVMF
                     LTGTDENAVKIERAAKEKGLTTQQFVDQLAVQWEDYFKKFGLSFDDFIRTTQPRHAAA
                     VKKAIQQLYDQGDVYKGSYEGWYCVPCETYFLESDLVDGKCPDCGRPVEKISEENYFF
                     KLTSYRDKLLKHYDENPDFVMPKARFNEVYSVIKEGLRDVSFTRTGVDWGIKAPFDEK
                     HVVYVWADALINYITAAGFESNPEGFKNIWPADVHLVGKDIIRFHCIIWPAMLMALGL
                     PLPKHIFAHGWWTCDGKKMSKSLGNFVKPHIEVENLVAISGCKEELAQDAFRYFLFRE
                     LPFGEDGDFSRANFEGRYNSDLANDLGNLLNRSQQMLQKNFEGIVPNGKVLSEASEKF
                     AETMKVFTERMEEFKFGQALEAIWGFINTLNTLIEVKKPWELVKQGKKEELADLHYTL
                     TDGIRVISALVNPFVFNTAKILANAVGLDTPPTIAGLALGQIKPGTITNKADVMFPRI
                     QKKQNLPAEKPAEPQKVEFVQQDEELMAIEDFQKFKFRVAKILSAEKVEGADKLLKLT
                     IDIGTEQRTIAAGIAKFYKPEDLIGKTIVVVANLKPAKLRGIISQGMLLAATDMETGN
                     VFVLSPEGPVVPGSMVK"
     gene            complement(3066..>3387)
                     /locus_tag="PK717_06255"
     CDS             complement(3066..>3387)
                     /locus_tag="PK717_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47295.1"
                     /translation="MKFSSMHRMELREDGFIYDITYNYPTPEHINSVKEIQNGNYFFR
                     RTICNCETVGLSKIALKNIPKGSECNDDDCRIYRDCLLLEKTYYVQSPEDAIKNWSIY
                     HPHR"
BASE COUNT          820 a          785 c          716 g         1066 t
ORIGIN      
        1 tcctttgaat gccaggtcat ctgggatcag ccaacaaaaa cggtaacgat aatctataac
       61 cgccgaggaa taacagttta tgttaccatt ggaagcaaga cagccttcat tgaataccgc
      121 cgcggtggtg gaaaaatacc gatcctgatg aaggacccac cggttattgt tggtggaaaa
      181 acgtttgttc cagtacaatt catggcccaa gcttttgatg ctaggtgggt aatgatctct
      241 gccagtcaga taaagataac gatcaagggt tcgtaataga aaaaaatctc cttgcgtttg
      301 ccgttgtaat ctcagccatc ttttctactg aacatttcct tatattcgct agccttatta
      361 gggtatgcct gatataggaa ggctcgtttc ttttgcctct caggggcaca ggggaaagat
      421 aagggcaatc tgtctcaacc atgatgaggt cttctggtgc aatggtggct gccatctcga
      481 gacgctcgtt tcttggatat gtcaacggcc cgccaaaaga aagatggtaa cctctttcaa
      541 atgcccattc tgccatgaga gtgtctcctg aaaaacaatg caggacaact gactttggcg
      601 aatgttcttc aagcattctc attacatcac cctgggcgtc tctgctatgt atcactactg
      661 gttttccaag attttttgct atctcgatct gctccgagaa tacttctcgc tgggtttgcc
      721 tgtatgatag atcacggtag taatcaaggc caatctcccc aatggcaaca accttttctc
      781 tgccagatag ctcaatgatc cagcttctga tctcggagcc ccattttttg gcttcatgcg
      841 gatggatgcc aactgtggca aaaatctctc tgttgttttg tgccagagat accgaccttg
      901 aagatgattc aaggtcaaat ccaacattta ttatctggca aacatcgccc tcttttgccc
      961 ttttaagcac cctttcaaac tcgcctttga aatcagggag gtcaagatgg gcatgagtat
     1021 caataatttc cattatttga ccatggaacc tgggaccacc ggaccttctg gagacaggac
     1081 aaaaacattt cctgtttcca tgtcggttgc ggcaaggagc attccctggg agattatgcc
     1141 gcgcaatttt gcaggcttca ggtttgcaac aaccacaatt gttttgccga tcaggtcttc
     1201 tggtttgtag aattttgcta ttcctgcagc aattgttctc tgttctgtgc caatgtcgat
     1261 tgtcagcttc aagagcttgt cggcaccctc aaccttttca gcgcttagga tttttgccac
     1321 cctgaatttg aatttctgga agtcttcaat agccatcagt tcttcatcct gttgtacaaa
     1381 ttcgactttt tgtggctctg caggtttttc ggcaggcagg ttttgctttt tctgtatcct
     1441 tgggaacata acatcagcct tgtttgtgat cgttcctggt ttgatctggc caagggcgag
     1501 gcccgctatt gttgggggag tgtctaggcc aactgcgttt gcgagtattt ttgcggtgtt
     1561 gaaaacaaaa gggttcacaa gcgctgaaat tacccttatt ccatcggtga gtgtgtagtg
     1621 gagatcggca agctcctctt ttttgccctg cttcacgagc tcccagggtt ttttgacttc
     1681 gatgagtgtg ttgagtgtgt tgatgaagcc ccatattgcc tcgagcgcct ggccaaactt
     1741 gaattcttcc atgcgctcgg tgaaaacctt cattgtttct gcgaacttct cgcttgcctc
     1801 agacagaact tttccgttcg gcacgatgcc ctcaaagttt ttctggagca tctgttgcga
     1861 cctgttcaga aggttgccga ggtcgtttgc aagatcagaa ttatatctac cctcgaaatt
     1921 tgcccttgaa aaatcaccat cttcaccaaa tgggagttcc ctgaacagaa aatatctgaa
     1981 agcatcttgt gccaattcct ctttacaccc cgagattgca acaaggtttt caacctcaat
     2041 gtgtggcttg acgaaatttc caagcgattt gctcattttt ttgccatcac atgtccacca
     2101 gccgtgggca aaaatgtgtt ttggtaatgg aaggccaagt gccatgagca ttgccggcca
     2161 tattatgcag tggaacctta tgatgtcttt ccctacgagg tgaacatcgg caggccagat
     2221 atttttgaag ccctcgggat tggattcaaa ccctgcggct gttatgtaat ttatcagcgc
     2281 atcggcccag acataaacca catgtttttc atcaaatggt gcctttatgc cccaatccac
     2341 ccctgttctt gtgaacgaga catcgcgcaa tccttctttg atgactgaat agacctcatt
     2401 gaaccttgct tttggcatta caaaatcagg gttctcgtca taatgcttca ggagcttgtc
     2461 cctgtagcta gtcagtttga agaagtaatt ttcttcagat attttctcga ctggtcttcc
     2521 gcaatcaggg cattttccat cgacaaggtc gctctcgagg aagtatgttt cgcatggcac
     2581 gcaataccag ccctcataac tccccttgta gacatcaccc tggtcgtaaa gctgctggat
     2641 tgcttttttg actgcagcag catgcctggg ctgggttgtc cttatgaaat catcaaacga
     2701 gaggccaaat tttttgaaat agtcctccca ttgtactgca agctggtcaa caaattgctg
     2761 tgttgtgagc cccttttctt ttgcagccct ttcgattttt acagcgtttt catcggtgcc
     2821 agtgagaaac atgacttcgc gtcccatcga cttcctgtac cttgcgataa tgtctgcagc
     2881 cattgttgtg tacacatgtc cgatatgcgg gttgtcgttg atgtagtaaa tgggcgttgt
     2941 cacataatac tttttcatct tgcacctcca aaaattgata ttcatgtcat tttatcggaa
     3001 ttgaggtgtt tttcaacgtt ttattacaga ttgccaatca ttcctacatt ccattggtgg
     3061 tggatttatc tgtgtgggtg ataaatgctc cagtttttta tggcatcttc tggcgactgg
     3121 acataataag ttttctccag caacaaacaa tctcgataaa ttctgcaatc atcgtcgttg
     3181 cattcactgc ctttagggat attcttcaat gcaatcttag aaagaccaac cgtttcacaa
     3241 ttacaaatag ttcttcggaa aaaataattt ccattctgta tctctttaac tgaattaata
     3301 tgctctggag taggataatt atatgtaata tcatatatga atccatcttc cctcagttcc
     3361 attctatgca ttgaactaaa cttcatc
//
LOCUS       DAOSRI010000194         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_25434_length_3363_cov_3.061366, whole genome shotgun sequence.
ACCESSION   DAOSRI010000194 DAOSRI010000000
VERSION     DAOSRI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_3363_cov_3.061366"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK717_06260"
     CDS             <1..264
                     /locus_tag="PK717_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47296.1"
                     /translation="RNRARSFGTSRRSEHLERHLRCAVAGHVPGVCSGRSSSGAGVRG
                     RDFPPQPTGDSGAMDLNWEYRYSGVSRITLSYSSGKWMIYDQQ"
     gene            251..1210
                     /locus_tag="PK717_06265"
     CDS             251..1210
                     /locus_tag="PK717_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47297.1"
                     /translation="MISNSLDVVYKPSWLSMVAAMESQLSFMGKEADYDWIAGITGFA
                     FRNAVEDQLLPGAFNFNFIWFDTFLSAYDRLGVCVEIISCHEHQLTFDAHQELLWEKI
                     CQSVENNLPPMVWENFEFGVVTGFDRELANWECAGVQGKLIHPRAKLGLGDVPFLFGV
                     IPVKNVQFDKTQATTMALRVAVSVGFLSKPPYPTGYNAVIGVEAYRKWQQELEKDFNL
                     YGCSHLIQTLKDARGSASLFLAKASELYDGITSTLLMRASERFAQVQHKLGEMSLLFP
                     YPGEFKAGEIERSKGKEMLDDCYIMETEGLKLIATALERVDIK"
     gene            1207..2034
                     /locus_tag="PK717_06270"
     CDS             1207..2034
                     /locus_tag="PK717_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOR47298.1"
                     /translation="MIDLHIHTELSPDAKGTFDQYISFAIEHGLKIVGFTEHWDFDRF
                     DPTCNFDVHKKSQEICRGYCGPIEVLYGAEIGYHRIYEDYVTKCLDEVKLDFILGSVH
                     EVEGLMISEVAETGEFFSKFGKKGFQVYFEAVANFAQDGIFDVLGHLDVIKRFSVLYD
                     FPFKEEQHKMQILEILDILVARNKALEINTSGLRQPPGASYPSLKVARWFFERGGKYI
                     TVGSDSHEPAMTGKGCDETIQNLINMGIIDITLFKERYPVVLNVIGEPGQGFVRKLR"
     gene            2045..3262
                     /locus_tag="PK717_06275"
     CDS             2045..3262
                     /locus_tag="PK717_06275"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47299.1"
                     /translation="MKKILTSLIVIATIVSVAPFMAFARTDEIHISTGQLVVGMSSEI
                     TIVVRETGTRALVQGAEVVMDGCGINTRRTTNAKGEAVFNVVPTETGKVKITATIQGM
                     RPTGTEIPVVPDKSPPPLDLDPLVPVTNQPSLTVVGRTRSGTEVYVGRVKAVVDEKGN
                     FKATVQLSEGDNHIVVKSSTQYASTTKEFLVTLDTRAPSVIIENDFGKEDYVDVKTIT
                     VKGRVDPGSKVTINGVEATVVNDFFICQIPVSPGKNTLTIVATDQVGNKTTIEKEILV
                     YTMKNIKITIGSTIGYVDGKETTLDAPPVLVGGRTMVPLRFIGEVFGAEVSFDSTTKQ
                     IFIHYEQKSIVLTIGSNQATIDGKVVTLDVPAQIVGGRTMVPVRFIADAFEANTSYDS
                     ATKTVTITKKILP"
BASE COUNT          944 a          736 c          851 g          832 t
ORIGIN      
        1 cggaatcgcg cacgcagctt cgggacttcc cggcggagcg aacatctgga aagacatctc
       61 cgatgcgcag tggcaggaca cgtaccgggt gtctgctcag gacgctcatc gagtggcgca
      121 ggggtacgcg gacgggactt tccgccccaa ccaaccggtg actctggcgc catggatctg
      181 aattgggaat acaggtattc cggggtttca agaattaccc tgtcttattc atccggaaaa
      241 tggatgatat atgatcagca atagtcttga tgttgtttac aaaccaagct ggctttccat
      301 ggttgctgcc atggagagcc agctttcatt tatgggcaag gaagccgatt atgactggat
      361 agcgggcata acgggttttg cctttaggaa tgccgtggaa gaccagctac ttcctggggc
      421 cttcaacttc aattttatat ggtttgatac attcctttcc gcatatgaca gacttggcgt
      481 gtgcgtggaa ataatatcct gccacgaaca ccagctgact tttgacgccc accaggaact
      541 cttgtgggag aaaatatgtc agtcggttga aaacaatctc ccgcctatgg tttgggagaa
      601 ttttgagttt ggagtcgtga ctggttttga cagggaactt gcaaactggg aatgtgccgg
      661 tgtacaggga aaactcatcc acccaagggc aaagcttggt ctaggagatg ttccgttcct
      721 tttcggggta atacccgtaa aaaatgtcca gtttgataaa acccaggcca caacaatggc
      781 attgagggtg gctgtttcag ttggtttcct gtccaagccg ccgtatccaa ccggatacaa
      841 tgccgttatt ggtgtagagg cttacagaaa atggcagcag gagctggaga aggactttaa
      901 tctttatggc tgttcccacc ttatacaaac cttgaaagat gccagaggat cggcctcgtt
      961 gtttcttgcc aaggcatcag agctctatga tggtattaca tcaacattgc tcatgagagc
     1021 ctccgaaaga tttgcccaag tccagcacaa acttggtgag atgtcactgc tgttccctta
     1081 ccctggtgaa ttcaaagctg gagagatcga gagatcaaaa gggaaagaga tgcttgatga
     1141 ttgctatata atggagacag agggtttgaa gctgatagcc acggcccttg aaagggtgga
     1201 tattaagtga ttgatctgca cattcataca gagctttcac cagatgcaaa ggggacgttt
     1261 gatcaatata tcagttttgc cattgaacat ggccttaaaa ttgtcggttt caccgagcat
     1321 tgggattttg accggtttga tccgacatgc aattttgatg tccataaaaa gagccaggaa
     1381 atttgcaggg ggtattgcgg gccaatcgag gtgctctacg gggcagaaat tggctatcac
     1441 aggatatatg aggattacgt cacaaaatgc ctcgatgaag tgaagctcga ttttatcctc
     1501 ggctctgtcc acgaagtcga aggcttgatg atatctgaag ttgcagaaac tggggaattt
     1561 tttagcaaat ttggcaaaaa gggttttcag gtttattttg aggccgtagc aaattttgcc
     1621 caggacggga tttttgatgt ccttggccac ctggatgtca taaaacgttt tagtgtcctt
     1681 tatgatttcc cattcaaaga ggaacagcac aagatgcaga tactcgagat actggacatt
     1741 cttgtcgcaa gaaacaaggc ccttgaaata aatacatccg gattgaggca accacccggt
     1801 gcctcgtacc ccagcctcaa agttgcaagg tggttctttg aaaggggcgg gaaatatata
     1861 actgtaggtt cggactccca tgaaccggca atgaccggca agggttgtga tgagaccata
     1921 caaaacctaa ttaatatggg aataatcgac ataacgctct tcaaagagcg ctaccccgtt
     1981 gtccttaatg ttattggtga accgggccag gggtttgtcc gtaaattgag gtgaaggagg
     2041 caagatgaaa aaaatcttaa cgagcctgat agtgatagcc acaatcgtta gtgttgcgcc
     2101 gtttatggct tttgcaagaa ctgatgagat acacatttca accggccagc ttgtcgtcgg
     2161 aatgtcatct gaaattacaa ttgttgtcag ggaaaccgga accagagcac tcgtgcaggg
     2221 tgctgaagtt gtaatggatg gttgcggcat aaacaccaga aggaccacaa acgccaaggg
     2281 agaagccgtt ttcaatgtcg tgcccaccga aactggaaag gtcaaaataa ctgcgacgat
     2341 acagggcatg aggccgactg gcaccgagat acccgtcgtt ccggacaaaa gcccgccacc
     2401 ccttgatctt gacccactgg tccccgtaac aaaccagcct tcgttgactg ttgttggaag
     2461 aacaagatcg ggtacagagg tctatgtagg cagagtcaaa gctgttgttg atgaaaaggg
     2521 caacttcaag gcaacagtcc aactctctga aggcgacaac cacattgttg tgaaatcttc
     2581 gacccagtat gcctcaacca caaaggagtt cctggtaaca ctcgacacaa gggcgccatc
     2641 ggtgatcatc gagaacgatt ttgggaaaga ggactacgtt gatgtaaaaa caatcactgt
     2701 gaagggcagg gtcgatcccg gttcaaaagt caccatcaat ggcgttgaag caacagttgt
     2761 aaatgatttt ttcatctgcc agataccggt gagccctggc aaaaacacgc tcacaatcgt
     2821 tgccacagac caggtgggca acaaaacgac aattgaaaaa gaaatcctcg tctacaccat
     2881 gaagaacatc aagataacaa taggcagcac cataggttat gttgatggca aggaaaccac
     2941 ccttgatgct cctcctgtac ttgttggagg caggacaatg gtcccccttc gcttcatcgg
     3001 tgaggttttt ggagcagaag tcagctttga ctcgactacg aaacagatat tcatccacta
     3061 cgaacagaaa agcattgttc tgacaattgg gtcaaatcag gccacaattg acgggaaggt
     3121 cgtcactctt gatgtgccag cccagattgt gggtggcagg acaatggtcc cggtgagatt
     3181 tattgccgat gcttttgagg caaacaccag ctatgactcg gcgactaaga ccgtcacaat
     3241 caccaaaaag atactgccat aaagattcct gatcagctat aattaaaaag ccccagcttc
     3301 atgctggggc tttttaattg ggtgattgca aatcttactg atgcctgtat tttaataaaa
     3361 tag
//
LOCUS       DAOSRI010000195         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_25694_length_3341_cov_4.395313, whole genome shotgun sequence.
ACCESSION   DAOSRI010000195 DAOSRI010000000
VERSION     DAOSRI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25694_length_3341_cov_4.395313"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK717_06280"
     CDS             <1..954
                     /locus_tag="PK717_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47300.1"
                     /translation="KIMVLKSYDQKGDVYVLDKEMPLVEYGENGFTFYKITEITKDGK
                     TFAIPAKDAKGNPVKLDPSAKNFFEEARKCFDGRFSCKFLGPSYDRGLYLGMVISNKD
                     NGFYYMKRLYHNRDQECSLMNFDMGPCYCKEKLPPGSCFFADGNFNVDIRKYDGEKLS
                     LYKMEAVNPDVRNECAPALYNFPVASWGGYIWMKIGERDTFVNGEKKALPAKVETRNG
                     TTMVPGKWVLENLTSSNTTSYDEKTKLWTFRRFIEKEWLEVKFTLGKKEFQIDGKKQQ
                     WVVAPYETVKGIPMVPLRQFCGIFQRELYWRSFDKTMLVKR"
     gene            complement(966..2906)
                     /locus_tag="PK717_06285"
     CDS             complement(966..2906)
                     /locus_tag="PK717_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOR47301.1"
                     /translation="MTDTRPDPNEILKRILEKEKDSEQEKKKGKLKVFLGLAAGVGKT
                     YRMLARAQMLKESGVDVVIGIIETHGRMETKKLAEGIETLPRKEIEYGGIKLEEMDID
                     AVIKRKPELILVDELAHTNAPGSRHNKRYLDVVELLENGIDVYTTLNVQHIESFNDIV
                     FQITGVRVKETVPDEILERAHHPDGIEVVDLPPEELIQRLKDGKVYVPEKSKQAIERF
                     FRKGNLLALREMALRYTARLVDEDIKTYMERHDIVGPWSAAPRLLVCVSPSPTSARLI
                     RLAKQLAPGIDAEWYAIHVEERNAPELTSDASETLTSNLRLAEELGGEVVTTVGSNIA
                     DEVIRFAREKNIIIILIGYGPRQKFSFTRKNLVDEVVRKSGNIHVLVVTDSEAAPVKS
                     TVANKLTFSYKHTFYSLLTLFVYATIFFFLGKWLNYANIVMLMTLPIIFSAIVWGRVA
                     GIINSIVGMVLIDLFFIPPLFQFTIEDINLLPSLLVYLVVGLTASFLAEMVRWQGKIA
                     RQREIFIESLYNLSRELLVFTNPKDITKKAVSEMDRVFECKTVFFEGVGDELAILSKS
                     DELEIHDKDKVIATWSLTHGGKTGKYTDTLPHAEFTFVPINIGSKTKYVAGFKFNQKL
                     TPEQSKLLDSFINVIATSLSIF"
     gene            complement(2912..>3341)
                     /locus_tag="PK717_06290"
     CDS             complement(2912..>3341)
                     /locus_tag="PK717_06290"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HOR47302.1"
                     /translation="EVIGSELIAQKFEAPFYFVPRPSAINYDAATSGGTSNGPTSEAL
                     KSEVVQNINVAKKMFGLKEGDQVPADMVTSSGSGLDPEISFDSAMIQAKSVANARKVA
                     ETEITKLVEEQATKGLFSPKRVNVLLLNLELDRLFPKGTK"
BASE COUNT          930 a          721 c          706 g          984 t
ORIGIN      
        1 aaaataatgg tgttaaaatc gtatgatcaa aaaggtgatg tttatgttct agacaaggag
       61 atgccgcttg tcgaatacgg tgagaatggt tttactttct acaaaatcac agaaatcacc
      121 aaagatggta aaactttcgc cataccagca aaagatgcca aaggcaatcc agtcaagctt
      181 gatccctcgg ccaagaattt ttttgaagag gccagaaaat gtttcgatgg cagattcagc
      241 tgcaaatttt tgggcccatc atatgacaga ggactgtatt taggcatggt catatcgaat
      301 aaagataacg gattctacta catgaagagg ctttaccaca atagagacca ggagtgttcg
      361 ctaatgaact ttgatatggg accgtgctat tgcaaagaaa aactgcctcc cggctcctgt
      421 ttctttgctg atggaaattt caatgtcgac attaggaaat atgatgggga aaaactgagc
      481 ctttacaaaa tggaagctgt caatccggat gttagaaatg aatgcgcacc agcgctgtat
      541 aatttccctg tggcttcctg gggtggctat atatggatga agattgggga acgagatacg
      601 tttgtaaatg gagaaaagaa agcccttcct gcaaaggtgg aaacaaggaa tggaacaacg
      661 atggttcctg gcaaatgggt cctcgaaaac ctcacaagct caaataccac ttcctatgat
      721 gaaaaaacaa aactctggac atttagaagg ttcattgaaa aagagtggct ggaagtcaaa
      781 tttacgcttg gcaaaaaaga attccagatt gatgggaaaa aacagcagtg ggtcgtggcc
      841 ccatatgaaa cagtaaaggg aattcccatg gttcctttaa gacagttctg tggaatcttc
      901 caacgagagc tctactggag atcttttgac aagacgatgc ttgtcaaaag ataatttaat
      961 aaatcctaaa agatcgaaag cgaagtagca ataacgttta tgaatgaatc aagaagtttt
     1021 gattgttctg gagtcaattt ctgattgaac ttaaaccctg caacatactt tgttttagag
     1081 ccaatattga ttgggacaaa tgtaaattcg gcatggggga gagtgtcggt gtatttgccg
     1141 gtttttcccc cgtgtgtaag cgaccacgtg gcaatgacct tatctttgtc gtgaatttca
     1201 agctcgtctg attttgagag aatggccagc tcatcaccaa caccttcaaa aaaaactgtt
     1261 ttgcactcaa aaactctgtc catttcagac actgcttttt ttgttatgtc ttttgggttt
     1321 gtaaacacaa gcaattcccg gctcaaattg tatagagatt caatgaaaat ctcgcgttgc
     1381 ctggctatct tgccttgcca tctgaccatc tcggccaaga aagatgctgt taatccaacc
     1441 acaaggtata ccagcaatga tgggagaaga ttaatgtctt caatggtgaa ttgaaatagc
     1501 ggtggtataa aaaacaaatc aatcagcacc attcctacga tggaattgat gatccctgca
     1561 acacgccccc agacaatggc cgagaaaatg atcggcaaag tcatgagcat cactatattg
     1621 gcatagttca gccacttgcc taggaagaag aaaatagttg catacacaaa tagtgtcaac
     1681 aatgaataaa aagtgtgttt ataagaaaaa gtaagcttat tagcaacagt tgatttgaca
     1741 ggcgccgctt cactgtccgt tacaaccaga acatggatat tgcctgattt tctgactacc
     1801 tcatcgacca ggttttttct ggtgaagctg aatttttgtc ttggaccgta gccaatcaaa
     1861 atgatgatga tgttcttttc ccttgcaaat cggatgactt catcagctat atttgaacca
     1921 actgttgtga caacttcccc acccagttcc tcagcaagcc tcaggtttga tgtaagcgtt
     1981 tctgaagcat cacttgtaag ctcaggagca ttccgctctt caacgtggat ggcataccat
     2041 tctgcatcaa tacctggggc caattgcttt gcaagtctta tcagcctggc agatgttggg
     2101 cttggcgaaa cacaaaccag cagtctgggt gcagccgacc atggcccgac aatatcatgc
     2161 cgctccatgt atgttttgat gtcttcatca actagcctgg cagtgtatct gagagccatt
     2221 tctcgaagag caagaagatt gccttttcta aagaaccttt caatggcctg cttggatttt
     2281 tctggaacat agactttgcc gtctttaagc ctttgtatca gctcttcagg tggtaaatca
     2341 accacttcga tgccatccgg atggtgtgct ctctcaagta tctcatcagg gaccgtttcc
     2401 ttgaccctta ctcctgtgat ctggaaaact atgtcgttga aagattctat gtgctggaca
     2461 ttcagtgtgg tataaacatc aatcccattt tctaggagtt ccacaacatc caggtagcgt
     2521 ttgttatgtc tcgaaccagg tgcatttgta tgcgcgagct catccaccaa gatgagttca
     2581 ggttttcttt ttatgacggc atcaatgtcc atctcttcca gcttgatccc gccatactca
     2641 atctcttttc tggggagagt ttctatgcct tcagcaagct ttttggtttc catccttcca
     2701 tgtgtctcta taatgccaat tacaacatca acgcctgact ccttgagcat ctgtgctctt
     2761 gcgagcatcc tgtaggtttt gccgacacct gcggcaagac caagaaaaac cttcagcttg
     2821 ccttttttct tttcctgttc gctgtctttt tctttttcaa gaatcctctt cagtatctcg
     2881 ttgggatcag gtctggtgtc tgtcatggct gttattttgt tcctttcgga aacaatctgt
     2941 caagttccag gttgagcaat aaaacattta ctctttttgg cgaaaacaaa ccttttgttg
     3001 cctgttcttc gaccagtttt gtaatctctg tttcagcaac ttttctggca tttgcaacag
     3061 attttgcctg aatcattgcg gaatcaaatg atatttctgg atcaaggcca gacccactgg
     3121 atgtcaccat atcggccggg acctggtcgc cctctttcaa gccaaacatt ttttttgcca
     3181 cgtttatgtt ttgcaccacc tccgatttca atgcctccga ggttgggcca ttgcttgtcc
     3241 ccccagatgt tgcagcatca taattgatgg ccgatgggcg tggaacaaag tagaatggtg
     3301 cttcaaattt ttgtgcaatc agttcagagc ctattacctc c
//
LOCUS       DAOSRI010000196         3338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_25725_length_3338_cov_4.465123, whole genome shotgun sequence.
ACCESSION   DAOSRI010000196 DAOSRI010000000
VERSION     DAOSRI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25725_length_3338_cov_4.465123"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK717_06295"
     CDS             <1..521
                     /locus_tag="PK717_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR47303.1"
                     /translation="QDVVYTAQYLQMARAKTACLLSTSFSCGFALQAKEELRNLAPQM
                     KQVGESAGMAFQIQDDILGLWGDPKKTGKSVGFDLIRHKKTLPISYAMENGSNSTKKL
                     IQEAYSKEISQKDAESLLSCLEVVGAKEYAMSQVREYTSKALSLLASIPTDEKLKKSL
                     GELIEGLVYRES"
     gene            518..1357
                     /gene="ispH"
                     /locus_tag="PK717_06300"
     CDS             518..1357
                     /gene="ispH"
                     /locus_tag="PK717_06300"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR47304.1"
                     /translation="MKVVLPEACQGFCFGVKRAIDEVTSFSKTGEVQVLGHLVHNPQV
                     VANLEHNGVHTIESPDQSTSGIVAITAHGAPKDVYNGFPNIRIIDCTCPFVKRLQKRV
                     AELSDEGYKVIIVGDSHHPEVVSVSSFAVDPIVVGNIGQAMEIGFFKKIAVLSQTTQD
                     TSLLRDVACELTGHTDLLLFENTICTSTRERQAQLLEVAGKCKAVVVVGGKVSANTKR
                     LYQLSKQKGAQTFWIEGPEELGEEVLIQIKTASGGGCVGLVSGASTPTSTVLQVFSTL
                     SSY"
     gene            complement(1317..>3338)
                     /locus_tag="PK717_06305"
     CDS             complement(1317..>3338)
                     /locus_tag="PK717_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47305.1"
                     /translation="IAVELEGEVITASDTEVIVKSKQGGIWRAEGSFGAKAGDSVYVV
                     GTADSNRWWTIASARLTVVGGDAPTSQIMSWGIVDSTECENNRLKIKLDDGTVYEIYP
                     EDLGVCEGFTQGERVQFSGVISHDRGRTVQGAKLARSGLSGATKTIVGIITEISCTSR
                     VIKMREEFAQSGGQGPIWTMGLAKDAECEKLKIGDRISVTGDPVPSQQYQLEDCKIIV
                     IGEEIKELKITGKLHTLNCASNIMIIESDGKLYRVFPSQESRCSDLFSGDTIEVTAKV
                     SIFRKYVLNDATWKKIYDKDAFKILQGKVDTATCPGIRFMDSTGEYWRVNIREDQPCN
                     LLDNGMEITLKGVLDLSQDHLMHKAEILDLMRPKVIYGTIDDISCSEGKLVVVDKANS
                     YWEVVMETSFGDCIANKFSKGDMVQVSGFQNMLYKDSPIYFGKVEGFGNETGMIPMDF
                     IGEVLNTDCGKGNIQVRSGHTTWIVTIPDGFNCNSLKPGDWVRVEGGREDWSKKICFG
                     NKIEVTVATMIGFISEANYAKNELTVVEIKKPEMKWTVSLADTSKLTSYKKGMFIKIS
                     GTVNPPNTRRITGASVVATFTQIKGKVTMVDHNKQLIKVTGTDLKEYTCYVKTDWVNL
                     EDYKLDQNIIVTGNITQSKDKTAEMKECLLEDNNPFFNMMRVLKKLATR"
BASE COUNT          833 a          854 c          721 g          930 t
ORIGIN      
        1 aacaggacgt ggtttacacg gcacaatacc tccagatggc aagggcaaaa accgcttgtc
       61 tcctgagtac aagcttctcc tgcggtttcg cattgcaggc aaaggaagag ctgaggaacc
      121 ttgcgccaca gatgaagcag gtcggcgaat cggccggcat ggcattccag atacaagacg
      181 acatccttgg cctctggggt gatccaaaaa agacgggcaa gtcggtcggt ttcgatctta
      241 tacgccacaa aaaaacactt ccgatttcat acgcaatgga aaatggcagc aactccacaa
      301 aaaagctcat ccaggaagct tattcaaagg aaatatcgca gaaagacgcg gaatcactcc
      361 tttcttgcct tgaggtggtt ggcgctaaag aatacgcaat gtcacaggtg agggaatata
      421 cctccaaggc attgtctttg ctggcctcaa taccaactga cgagaaactc aagaaatccc
      481 ttggtgaact catagaaggg ctggtctaca gggaatcatg aaggtcgtcc tgcccgaagc
      541 ctgccagggt ttctgtttcg gtgtaaagag ggccattgac gaagtcacat ccttctcgaa
      601 gactggtgag gttcaggtcc ttgggcacct tgtccataac ccccaggtgg ttgcaaacct
      661 ggaacacaac ggcgtccata ccattgaatc tccagaccag tcaacatcag gcatagttgc
      721 cataaccgca catggcgccc caaaagatgt atacaatggg tttccaaaca tcagaataat
      781 tgactgtaca tgcccatttg tcaaaagact gcaaaaaagg gtggcggagc tttccgacga
      841 gggttacaag gtaatcatcg tcggtgattc acaccatccg gaggtcgtct cagtttccag
      901 ctttgcagtt gaccctattg tcgtaggtaa tataggccag gccatggaaa ttggcttttt
      961 caagaaaata gctgtgctct cccagaccac gcaagacacc tcgctgctca gagacgttgc
     1021 ctgcgaactg accgggcaca ctgaccttct cctttttgaa aacaccatct gtacttcaac
     1081 cagggaaaga caggcacagt tgcttgaagt tgccggaaaa tgcaaggctg ttgtcgtagt
     1141 tggtggcaag gtttcggcca atacaaaaag actctaccag ctttcaaaac aaaaaggggc
     1201 ccaaaccttt tggatagagg gccccgaaga acttggcgaa gaagtgctca tccagataaa
     1261 aactgcgtct ggtggcgggt gtgttggcct cgtttcaggg gcatctacac caacttctac
     1321 cgtgttgcaa gttttttcaa cactctcatc atattgaaaa acggattgtt atcttcgagc
     1381 aaacactctt tcatctctgc tgttttgtct ttgctttgcg ttatatttcc tgtcacaatg
     1441 atattctgat caagtttgta atcttccaga ttgacccagt cggttttgac atagcatgta
     1501 tattccttga gatcagtgcc ggttactttg atcagttgtt tgttgtgatc aaccattgtg
     1561 accttcccct ttatctgggt aaatgtggcc acaacgcttg cgccggtaat gcgtcttgtg
     1621 ttcggagggt tgactgttcc agatattttt atgaacattc cctttttgta tgatgtcagc
     1681 ttgcttgtgt cggcaagcga aacggtccac ttcatttcag gtttctttat ttcgacgact
     1741 gtgagctcgt ttttggcata attggcctcg gaaataaatc caatcattgt ggcaacagtc
     1801 acttcaattt tgtttccaaa acagatcttc tttgaccagt cttccctgcc accctcgacc
     1861 ctgacccagt cgccaggctt tagactgttg caattaaaac catccggaat agtcactatc
     1921 caggtggtgt gcccggaacg aacctggatg ttgcctttgc cacaatcagt attcaggacc
     1981 tcaccaatga aatccattgg tatcattccg gtttcattgc caaatccttc cactttccca
     2041 aaatatatcg gggagtcttt gtaaagcatg ttctggaaac cactgacctg caccatgtcg
     2101 cccttggaga atttgtttgc aatgcaatcg ccaaaggatg tttccattac aacttcccaa
     2161 taggagtttg ccttgtccac gacaacaagt tttccttcgg aacaggatat gtcgtcaatt
     2221 gttccgtaga taacttttgg cctcataagg tccagtattt cggccttgtg catgaggtgg
     2281 tcctgagaaa ggtcaagcac gccctttaac gttatctcca tgccattatc aagaaggttg
     2341 caaggctgat cttccctgat gttgaccctc cagtattcac ctgtcgaatc catgaacctg
     2401 atgccagggc aggttgcagt gtcaactttt ccttgcagta ttttaaatgc atccttgtcg
     2461 tagatcttct tccatgttgc atcattgaga acatatttcc tgaagatcga aacctttgcg
     2521 gtcacctcaa tcgtgtcccc tgagaaaagg tctgaacatc ttgattcctg tgatgggaac
     2581 actctgtaca gcttgccgtc agattcaatt atcatgatat tcgaggcaca attgagagtg
     2641 tggagctttc ctgttatctt tagctccttt atctcctcac ctatcactat gatcttgcaa
     2701 tcctcaagct gatattgctg tgagggcacc ggatcgccag ttacactgat cctgtctcca
     2761 atcttgagct tctcgcactc tgcatctttt gcgagaccca ttgtccatat cggcccctgc
     2821 ccaccactct gagcaaattc ctccctcatt tttataaccc tggaggtgca agagatctcg
     2881 gttattatgc caactattgt ctttgttgcg cctgataagc cagatctggc aagcttcgca
     2941 ccctgcacgg tcctgcctct gtcgtgcgaa atcactccag agaactgcac tctttcaccc
     3001 tgggtgaaac cttcacagac accaagatct tccgggtaaa tctcgtatac tgttccatca
     3061 tcaagcttta ttttgagcct gttgttttca cattcggttg aatcaacaat gccccaggac
     3121 attatctggc tggttggggc atcgccccca acaacagtca gcctcgcaga agcgattgtc
     3181 caccatctgt ttgagtcggc agtcccgaca acataaacgc tgtcgcccgc ttttgcgcca
     3241 aatgagcctt ctgccctcca tattccaccc tgttttgact tcactatcac ttcagtatca
     3301 gatgctgtta taacttcacc ctcaagttcg acagcaat
//
LOCUS       DAOSRI010000197         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_25797_length_3330_cov_4.224122, whole genome shotgun sequence.
ACCESSION   DAOSRI010000197 DAOSRI010000000
VERSION     DAOSRI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25797_length_3330_cov_4.224122"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK717_06310"
     CDS             complement(<1..958)
                     /locus_tag="PK717_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47306.1"
                     /translation="MKKWLSFVVIFSLIMMPIPTMGISSQPLDSSCDAYGNTFVLDIS
                     GQKVLKFDKNLNYQQTIISQSQLQQMRASSITVCWCAGEISFVSSSTSQTKMFEIDPK
                     SNYVFKRNILPVGVAKGQTKNPSEIVYLRSNDDYWVAIVDPQLKKIAVVDDYGKFRYE
                     IMGLSSPKACYFSTSKQIYAVDSQSIKKYSTSGSYLSSFQSEILNSPIAIDASRNEDI
                     FFVLDGQNVKVFDKDGNYLRKFAVGASSCSLCVNQDKDWVIVSSSSDGGTLSAYDYLG
                     KLQKKVTNAANPKKEKVIIFTIGSYVGYVDGVGTKLRCPTKIE"
     gene            complement(1060..1983)
                     /gene="argF"
                     /locus_tag="PK717_06315"
     CDS             complement(1060..1983)
                     /gene="argF"
                     /locus_tag="PK717_06315"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOR47307.1"
                     /translation="MKGKSVLSIHDLTIEEVEQIFDVARTLKLERMVGKLINPILAGK
                     TLAMIFEKPSTRTRLSFDIAMHELGGHPIYLSSNDLQLARGESIADTAKVLSRYVHGI
                     MARVFQHQKLVDLARYGSVPVVNGLSDLLHPCQVLADLFTVREKFGSFCGRKLVYMGD
                     GANNMANSLIEGCAKVGMDCVICTPKGDLPDKTIWDNALQDAELTGATIEVSHDPIEA
                     VQNSDVIYSDVWASMGQEAEHAERVKRMMPYQVTPKLMEVSGGALFMHCLPAHRGEEV
                     VDEVVDAPYSIVFDEAENRLHVQKAVLALVL"
     gene            complement(1995..3305)
                     /locus_tag="PK717_06320"
     CDS             complement(1995..3305)
                     /locus_tag="PK717_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOR47308.1"
                     /translation="MARKVIIMGAAGRDFHNFNMLFRDNPDYEVVAFTATQIPDIAGR
                     KYPVELTGKLYPNGIPIYDESELPRLIKDLKADEVMFAYSDQPHEVVMNKGSMVIANG
                     ADFVLAGWQKTALKSSKPVISVTAVRTGCGKSQTTRAVIEVLRGMGKKVVSIRHPMPY
                     GDLAKQAVQRFATYADLDTHKCTIEEREEYEPHIDLGAVIYSGVDYGAILKEAEKEAD
                     IVIWDGGNNDLPFIMPNLAIVVVDPLRPGHEISYHPGEANLLIADTVVINKIDSAYPE
                     DVEIVRSNIFQRNPKAMIVEATSPVFVEKPELIKGKRVLVVEDGPTLTHGEMTFGAGF
                     VAARKAGAGEIIAPKPFAVGSIAATFEKYCHVENVLPAMGYSPEQIKDLKETIDAVPC
                     DVVVSATPIDITRVLKTNKPIVRVGYELGVFGKPTLEEIIKGKF"
BASE COUNT          877 a          746 c          722 g          985 t
ORIGIN      
        1 tttcaatctt tgtaggacaa cgcagttttg tgccaactcc atccacataa ccaacatagc
       61 tgccaattgt aaaaatgatg accttttctt tctttggatt ggcagcattt gttacttttt
      121 tctggagttt ccccaaatag tcgtatgcag acagtgttcc accatcggat gacgaagaaa
      181 caatcaccca atccttatcc tgattgacac aaagagaaca ggaggaagcg ccaacggcaa
      241 atttcctcaa ataattccca tctttgtcaa acactttgac gttttggcca tccagaacaa
      301 agaaaatatc ttcatttctt gaggcatcta ttgcaatcgg tgaatttaat atctcactct
      361 gaaagctcga aagatacgac ccagaagtag aatatttttt gatgctttgt gagtctaccg
      421 catagatttg ctttgatgtt gagaaataac atgccttggg cgaagagaga cccattatct
      481 cgtatctaaa ctttccatag tcatcaacca cagcaatctt cttaagttga ggatccacaa
      541 tagccaccca ataatcgtca tttgaacgca aataaacaat ttctgaagga ttttttgttt
      601 gtcccttggc tacacccaca ggcaatatat ttcttttaaa aacatagtta cttttgggat
      661 caatctcaaa cattttggtt tgggatgttg aagatgaaac aaaagagatt tcgccagcac
      721 accagcaaac agtgattgag cttgccctca tttgctgtag ttgtgattgc gagatgatag
      781 tttgctggta attaaggttt ttatcaaact tcaggacttt ttgacctgaa atatccaata
      841 cgaaggtgtt tccgtaagca tcacaacttg aatccagtgg ttgggatgat atacccatgg
      901 tgggtatagg catcataatc aaggaaaata ttacaacaaa cgacaaccac tttttcatat
      961 ttaatttttt atcctctttg aatatttttg tcaagagcca aatggggggg tatataaaaa
     1021 aaggccccta aaaaggggcc tttttgttga aaatggttat cagaggacaa gcgccaaaac
     1081 tgctttttga acatgcagcc tgttttctgc ctcgtcaaaa acgattgagt atggggcatc
     1141 aacaacctca tcgacaactt cttcacccct gtgcgctggc aggcagtgca tgaagagggc
     1201 tccaccgctc acttccatga gttttggtgt tacctgatac ggcatcatcc tcttgactct
     1261 ctcggcatgc tcagcttcct ggcccattga ggcccagaca tcagagtaga taacatcaga
     1321 attctgaacg gcttcgattg gatcatggct gacttcgatg gttgcaccgg tgagctctgc
     1381 atcctgaagt gcattgtccc agatagtttt atcaggaaga tcgccctttg gtgtgcagat
     1441 aacacaatcc attccaactt ttgcgcaacc ttcgataagg ctatttgcca tgttgttggc
     1501 gccatcgccc atatagacga gctttctgcc acagaacgag ccaaattttt cccttaccgt
     1561 gaaaaggtcg gcaagcacct ggcatgggtg aagaagatca gaaaggccgt tgaccactgg
     1621 aactgagccg tatcttgcaa ggtcaacaag tttttggtgc tggaaaaccc tggccataat
     1681 tccatgaaca taccttgaaa gaacttttgc agtgtcagcg atagattcgc cacgggcaag
     1741 ctgaaggtca tttgaggaga gatatatcgg gtggccacca agctcatgca tggcaatgtc
     1801 aaaagataat ctggtacgcg tggagggctt ttcaaaaatc attgcaaggg ttttgcctgc
     1861 aaggattgga ttgatgagct ttccaaccat tctttcaagt ttgagtgtgc gggcaacatc
     1921 aaatatctgt tcgacttctt cgatggtgag atcgtgaatg gaaagaactg actttccttt
     1981 catgtttatt gccatcagaa tttccccttg atgatttctt cgagggtcgg ctttccaaac
     2041 acgccaagct catatcctac tctgacaatt ggtttattgg ttttgaggac cctggttata
     2101 tcgattggcg ttgcagagac aacaacatcg catggcactg catcgatagt ctctttgagg
     2161 tccttgatct gttctggtga ataacccatt gctggaagta cattttcaac gtggcagtac
     2221 ttttcgaatg ttgcagcgat agaaccaact gcaaatggct tcggtgcgat tatttctccg
     2281 gctcctgctt ttcttgcggc gacgaatcct gcgccgaaag tcatttcgcc atgagtgagt
     2341 gttgggccat cttcgacaac caagaccctc ttgcccttaa tcaattctgg tttttcaaca
     2401 aaaactggtg aggttgcttc aacaatcatg gcctttggat tgcgctggaa gatgttgctt
     2461 ctgacgattt caacgtcctc tgggtatgcc gagtcgatct tgttgattac gacagtatcg
     2521 gcaatcagaa ggtttgcctc tccgggatga taggatattt catggcctgg cctgagtgga
     2581 tcaactacca cgatggcaag attcggcatg atgaatggca gatcattgtt tccaccgtcc
     2641 cagataacga tatcagcttc tttttctgct tccttgagaa tggcgccata atcaacgcca
     2701 gagtagatta ctgctccaag atcgatgtgt ggctcgtatt cttctctctc ttcaatggta
     2761 catttgtggg tatcaagatc agcataagtt gcaaatctct ggacagcctg ctttgcaagg
     2821 tcgccgtaag gcattggatg tcttattgaa acaacctttt tgcccatacc acggaggact
     2881 tcaataacag ctctggtggt ctgactcttg ccacaaccag tcctgacggc agtaaccgag
     2941 atgaccggct ttgaagactt gagggcggtc ttttgccatc cagcgagcac aaaatctgct
     3001 ccattggcaa tgaccatcga gcccttgttc atgacaactt cgtgtggctg gtctgagtag
     3061 gcgaacatga cttcatcggc cttgaggtct tttatgaggc gtggaagctc cgattcgtca
     3121 taaatcggta ttccatttgg ataaagctta cctgtaagtt caaccggata ttttcttcca
     3181 gctatgtccg ggatttgtgt ggccgtaaaa gcaacaactt cataatccgg attgtctcta
     3241 aagagcatat tgaagttgtg gaaatctctt ccagcggcac ccatgatgat tacttttcta
     3301 gccattgtta ttatgcctcc actatttctt
//
LOCUS       DAOSRI010000198         3292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_26203_length_3292_cov_3.857584, whole genome shotgun sequence.
ACCESSION   DAOSRI010000198 DAOSRI010000000
VERSION     DAOSRI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26203_length_3292_cov_3.857584"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK717_06325"
     CDS             complement(<1..250)
                     /locus_tag="PK717_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47309.1"
                     /translation="MNKRTIALVVAGVLALASIILDQLGLIEGLKNGFFIYILISILI
                     IAIGIASKNKGILMASIVFGVALSVGKTISMGYVPLENL"
     gene            346..1575
                     /locus_tag="PK717_06330"
     CDS             346..1575
                     /locus_tag="PK717_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOR47310.1"
                     /translation="MSRIYDEKLKDAGLLKIDWVSKRMPVLTNLSEYLKGKFSGVKIS
                     MSIHLEAKTAYLAKVLKLSGAEVAICSSNPLTAQDDVAAGLASLGVNVFARRGESETE
                     HEENIESTLAINPDLVLDDGADLVVTMVRKHPNMKVLCATEETTSGLTRMRALAREGK
                     LPCPVIAVNDAQSKYLFDNRYGTGQSSWDGIMRTTNLTVCGKTVVIGGYGWVGRGLAM
                     RAKGLGARVVATEVNPIRAMEAYFDGCEVMEMKDAARIGDIFVTATGDINIIDGRHFD
                     LLKDGAILCNAGHFDVEVSMAQLRNYAVSHREARNNIQEYMLSSGKKIYVLGEGRLVN
                     LVCADGHPAEIMDLSFSLQSLSLLYGLENGKSLAKEVVPVPQKVDMDVARLKLESVGV
                     KVDQLTEEQVRYLASWE"
     gene            complement(1554..1961)
                     /locus_tag="PK717_06335"
     CDS             complement(1554..1961)
                     /locus_tag="PK717_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47311.1"
                     /translation="MDKTEVSQIKTSGIILGITFLFIPIVALIYSLATFKKNEKILIA
                     QWGKVSAENKEKLRTMSSALLATWHAAFAQLGLSLIVAAFLGYMYGFDALNKTASVNT
                     FMFFFMVAAVIVQVWAGVNGFILSRNTYSQEAK"
     gene            2148..3089
                     /locus_tag="PK717_06340"
     CDS             2148..3089
                     /locus_tag="PK717_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HOR47312.1"
                     /translation="MEKTYTETDILGSIRKINHAIAKRSKLAISKSGFTLPQLSVMQE
                     LLRKGPMTASELSNFVSISISTLTGVVSRLEAKKMVKKNQSKRDRRSVVISITKKGEE
                     KLDDLPPLGLEDALESLGSWDKKIFGPMVSKLASALETDGLAKSGDDGPMLVGTFDGD
                     CGKVDIYRVDSLGSLEFFTTADGLARFIMDALHPYEDTFEDTLRGIDDAIGGDPRKGG
                     FIVLAVQNKKVLGVVVMLETRMQGYIPDKCLLFVGVDQNNRSHGIGRKLIETAVKESR
                     GDIYLHVEYANKGAQRLYERIGFVNRYAEMRYYKHSK"
BASE COUNT          947 a          630 c          810 g          905 t
ORIGIN      
        1 tcaaattttc aagtggtacg tatcccatgg aaatagtttt cccaacagaa agtgccaccc
       61 caaaaacgat tgatgccatt agaatgccct tgtttttgct tgcaattcca atggctatga
      121 tgaggattga gattagaatg tatataaaaa agccattttt caggccctct ataaggccaa
      181 gctggtcgag tattattgat gcaagggcaa gaacgccagc gacaacaagg gcgattgttc
      241 tcttgttcat gcaaagcatt ctagcagggc ttttaccctt ttcaatgcaa acttttaaat
      301 ggaaaagaaa tctgctattt tgttgtttgc ataaggagga gtttaatgag cagaatctat
      361 gatgaaaaac taaaagatgc aggccttttg aagatagatt gggtttcaaa aagaatgcct
      421 gtcctcacga atctttcgga atatctaaaa ggcaagttta gtggggtaaa aatctccatg
      481 tccatacatc ttgaggccaa aacagcttat cttgcaaagg tactcaaatt atcaggtgcc
      541 gaggttgcaa tttgttcatc aaatcccctc actgcacaag acgatgttgc tgctggcctg
      601 gcctcccttg gagtaaatgt ttttgcaaga agaggtgaaa gcgagaccga gcatgaagaa
      661 aacattgaga gtaccctggc aattaatcct gatcttgtgc ttgatgatgg tgctgatctt
      721 gttgtgacaa tggtcagaaa gcacccaaat atgaaagtcc tttgtgcaac agaagaaaca
      781 acctctggtt tgaccaggat gagggcgctc gcccgtgagg gcaagctgcc atgtcctgtg
      841 attgccgtga atgatgccca gagcaaatat ctttttgaca acaggtatgg aacaggtcag
      901 tcatcctggg atggaataat gaggacaacc aacctgacag tgtgtgggaa aacagtggta
      961 ataggcggct atggctgggt gggcagggga cttgcaatga gagcaaaagg tcttggcgcc
     1021 agggttgtag cgactgaagt taaccctatc agggcaatgg aggcctattt tgacggctgt
     1081 gaggtcatgg agatgaaaga tgcagccaga attggcgaca tctttgtgac cgcaacaggt
     1141 gacatcaaca taattgatgg tagacatttt gatctgttga aagatggggc aatcctttgt
     1201 aatgccggac attttgatgt ggaagtgtcg atggcccagc tcagaaacta tgcggtttct
     1261 cacagagaag ccagaaacaa tatccaggaa tatatgctgt cttctggaaa aaaaatctat
     1321 gtgcttggcg agggaagact tgtcaatctg gtgtgtgccg atggccaccc tgcggagatc
     1381 atggacctct cgttttcttt acagtcactt tccctgcttt atggtctgga aaatgggaaa
     1441 agcctggcaa aggaagtggt ccctgttcca caaaaggtgg atatggacgt tgccaggctc
     1501 aaattggaat cagttggagt aaaggtcgac cagttgactg aagagcaagt tcgttatttg
     1561 gcttcttggg aataggtgtt cctggagaga ataaatccat ttactccggc ccagacctga
     1621 acgattactg cagcaaccat gaagaagaac ataaatgtgt ttacagaggc ggttttgttc
     1681 aatgcatcga acccatacat atagcctaga aacgctgcta caatcaatga aagcccaagc
     1741 tgggcaaatg cagcatgcca tgttgcaagg agtgcagagc tcattgttct gagtttctcc
     1801 ttgttttcag cagaaacctt gccccactgg gcaataagga ttttctcgtt tttcttgaac
     1861 gtggcgagtg aatagatgag tgccactatt ggtatgaaaa gaaatgtaat tccaagtatg
     1921 atacctgatg ttttaatctg tgaaacttcg gttttgtcca tgttgtacct ccacgccaat
     1981 tatatcttgc gcaagatcgt tcttcaagct gcagtgagat gatttcaact gaaatcaaaa
     2041 tgtgtaaaat catgctattt acataattaa atctggctca tttaaatttt gtaataaaca
     2101 gcattttatg tataatgtta ccaatcaaaa aaattaaagg acaatttatg gaaaaaactt
     2161 ataccgaaac tgacatcttg ggctcaatca gaaagatcaa tcatgcaatt gcaaaaagat
     2221 caaagcttgc gataagcaaa tcagggttta ctttgccgca actctctgtc atgcaggaac
     2281 ttctacgcaa gggccccatg actgcaagcg aactctccaa ttttgtttca ataagcattt
     2341 ctaccttgac tggtgtggta agcaggcttg aagcgaaaaa gatggtgaag aaaaaccagt
     2401 caaagcgtga caggcgatct gtcgtcattt cgataaccaa aaagggagaa gaaaaacttg
     2461 atgatctccc gcccttgggg cttgaagatg ctcttgagtc acttgggtct tgggacaaga
     2521 aaatttttgg cccaatggtt tccaagcttg catcagcgct tgaaacagat gggttggcca
     2581 aaagtgggga tgatggaccc atgcttgttg gcacatttga tggcgattgt gggaaagtag
     2641 atatctatag agtcgattcg ctgggttcgc tagaattctt tacaacagcc gatggccttg
     2701 cgcgttttat tatggatgcc ctccaccctt acgaagatac ttttgaggac acgctaaggg
     2761 gtatagatga tgccataggt ggtgatccaa ggaagggcgg ttttattgtc cttgctgtcc
     2821 agaacaaaaa ggtgcttggt gttgtggtga tgctcgaaac gagaatgcag ggctacatcc
     2881 ctgacaagtg ccttcttttt gtcggggtgg accagaacaa tcgtagccac gggattggta
     2941 gaaaactcat agaaacggcc gtaaaggagt ccaggggcga tatttatctg catgtcgagt
     3001 atgccaacaa gggtgcccag cgcctttatg agaggatagg ctttgtaaac aggtatgccg
     3061 agatgaggta ttacaagcat tcgaaataat ctgggttttg ccttgatatt aaaaaggccc
     3121 tccattatgg agggcctttt taattagttg ttacctaatt tatttcatgt ggacgattct
     3181 ttcgaccttt ttattaaggc cgaatgcatc gtcaacagag ataatcactt tgtaatcgcc
     3241 agctgaggtg taggtgtgcg ttagtgtgtg cggctcttca tttattgcgg tg
//
LOCUS       DAOSRI010000199         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_26453_length_3269_cov_3.274113, whole genome shotgun sequence.
ACCESSION   DAOSRI010000199 DAOSRI010000000
VERSION     DAOSRI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26453_length_3269_cov_3.274113"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..874
                     /locus_tag="PK717_06345"
     CDS             116..874
                     /locus_tag="PK717_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47313.1"
                     /translation="MNNVPEHIIPVKEGRITESELLQFMDYKDMMICSECYEPYGFWA
                     YRPEIIDYLPDEWKPVKRYFQKCDSTCSQHNQKPQMPDEDREQALWPGFDFNERLTLC
                     HACGQEIRLSGSRFSYWFCRTCLGRALYFNSQHAPVIIPQGRHSSVAGFVLPGMDRRN
                     EEKIREFVLDTNRLFRSITHLDVWRRFVMKTNFRIMGISGDVRLDYYLASAYSDQLKF
                     EAWGELCNFMMKKYKEDEGYEDPHINVSEERREV"
     gene            871..1647
                     /locus_tag="PK717_06350"
     CDS             871..1647
                     /locus_tag="PK717_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOR47314.1"
                     /translation="MIGAIAGDIIGSVYEHDNITRTDFPLFNSRCRFTDDTVLTVAVA
                     DCLLHGKSYAETYREYFHRYPFSGYGGSFIRWADLPRRGPYYSFGNGSAMRAGPVGWA
                     FNNLEEVLAEAKRSAEVTHNHPEGIKGAQSVAAAIFLARTGVSREDIGAFITKTFGYQ
                     LDEPIDEIRKHYRFDVTCQGSVPQAIRAFLESDGYEDAVRKAISIGGDSDTIACITGG
                     IAEAFYKGVPQEIVSFAMEKLDNDLRQVVIEFQDRFMKTR"
     gene            2084..>3269
                     /locus_tag="PK717_06355"
     CDS             2084..>3269
                     /locus_tag="PK717_06355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOR47315.1"
                     /translation="MKFIHAADIHLDSPLIGLERYDGAPIDEVRGATRKALQNLVERA
                     VHEKVDFVLIAGDLYDGDWKDYNTGLFFVSQMTKLRDSGIKVIIVTGNHDAASQITKN
                     LRIPDNCRMLSVHKPESVVFESLGVAIHGQGFSTRAVTDNLVAHYPKARPGLFNIGLL
                     HTSLNGREGHEPYAPCTLDELLSRGYGYWALGHVHKREIVHQEPWVVFSGNSQGRNAR
                     ETGEKGCMLVSVSDGILTDIKFISLDIFRWSVIDVDVTGLSEIDSILNTLDKALKREL
                     QVNDNMPLALRICLSGSSRLHGKILARAEHLKNEIRGCAIDVGYGNIWVEKVCIETSG
                     MSDPETLMMRDDALGGLLRSISDLQYDGKSLDDMLGELGDLFRKLPAEYLDDMEAIHP
                     ERK"
BASE COUNT          876 a          693 c          875 g          825 t
ORIGIN      
        1 ttcgaaaatg taagctctcc ggcaatcgta tgtcatatga accgtccctg atgtggacgg
       61 tttttttgtc tcctcgcctg gtgtgatgca aatacaacaa gaggaggaac acagtatgaa
      121 caacgtaccc gaacacatta ttccggtaaa agaaggacga atcactgaat cagaactcct
      181 tcagttcatg gactacaagg acatgatgat ctgctccgag tgttacgaac cctatggatt
      241 ctgggcgtac cgccctgaga tcatcgacta cctgcccgat gaatggaaac cggtaaaacg
      301 gtatttccag aagtgtgaca gcacatgcag ccagcataat caaaaaccac agatgcccga
      361 tgaggacagg gaacaggccc tatggccggg ctttgatttt aacgaaaggc tcaccctctg
      421 ccatgcatgc ggccaggaga tacgcctgag tggctcacgg ttctcctact ggttctgcag
      481 gacctgtctg ggacgggctc tgtacttcaa ctcccagcat gcaccggtca tcattcccca
      541 gggccgtcac agctccgttg ctggctttgt cctgccggga atggacagac gcaatgaaga
      601 gaagatcaga gagttcgtac tggacacgaa ccgcctcttc agaagtatca cccacctgga
      661 tgtttggaga cggttcgtca tgaagaccaa cttcaggatc atggggattt ccggtgatgt
      721 gcgtctggat tactaccttg ccagcgctta cagtgaccag ttgaagtttg aagcatgggg
      781 agaactgtgc aatttcatga tgaagaagta taaagaagat gaaggatatg aggatccaca
      841 cataaacgta tcggaagaaa ggagggaagt atgatcggtg ccattgcagg agatattatc
      901 ggatcagtat atgaacacga caacataacc agaacagact ttccactctt taattcccgc
      961 tgtcgattca ccgatgatac ggtgctgact gttgcagtag cagattgcct gctccatgga
     1021 aaaagctatg ccgaaactta ccgtgaatac tttcaccgct atcccttttc aggatacgga
     1081 ggaagcttca tacgttgggc agatttaccg agaagagggc cctactatag ttttgggaac
     1141 ggatctgcca tgcgggcagg ccctgttgga tgggcattca ataacctgga agaggtactc
     1201 gctgaggcaa aacgaagtgc cgaagtaacg cacaatcacc ctgaggggat caagggtgcc
     1261 cagtcagttg ctgctgccat atttcttgcc aggacagggg tatccaggga ggatattgga
     1321 gctttcatta cgaagacctt tggctatcaa ctcgatgagc ccatcgatga gattcggaag
     1381 cactaccggt tcgatgttac ctgtcagggg tcagtccccc aggccatcag ggccttcctg
     1441 gaatctgacg gatatgagga cgctgtccgc aaggccatct ccatcggtgg tgacagcgat
     1501 accatcgcct gtattaccgg aggaatcgca gaggctttct ataagggtgt gccgcaggag
     1561 atagtaagct ttgctatgga aaaactggac aatgacctac ggcaagtagt cattgagttt
     1621 caagacagat tcatgaaaac tcgatgaggt cagtccataa aattatagag atatggaaat
     1681 gataaacact aatgaaggta gcgtcatcgg aatggaggga tagtatgtct tttagaatgt
     1741 aaaaacacag atgtcaaaaa gacggaaaaa tgtcttggca gggggaatct tggtactgtt
     1801 ccaagtgtgg aacaatgagc gagtatgatg gttggcacaa ctgtatgcat gaggccatgg
     1861 cccgggggta tctgttgcgc tatggtttga tgccaatagg tccccatcgg caattggctg
     1921 acaaagtaat gtctggtgct tatcaaacat gtatcgggtg cgcagggtat tggttgttaa
     1981 tctttccagt acatcttccg cttcgcccat gtcttgattt cttttttgtg gtagaacttc
     2041 aactgtttct tgtgttaata gtgttagtct ggagaggata ggaatgaaat tcattcatgc
     2101 tgctgatatt catctcgaca gcccattgat agggctggag aggtatgatg gtgctcccat
     2161 agatgaagta cggggcgcaa cacgaaaggc gctacagaac cttgttgagc gggctgttca
     2221 tgaaaaagtc gattttgtgt tgattgcggg cgacctttat gacggagact ggaaggatta
     2281 caacacaggg ttatttttcg tgtcccagat gactaagtta cgggactcag gtatcaaagt
     2341 aattattgtg accggaaacc acgatgctgc aagccagatt accaagaatc ttcgtatccc
     2401 ggacaattgc agaatgctct cagtgcacaa gcccgaatcg gttgtctttg aaagcctcgg
     2461 tgtggccatt catggacagg gattttcgac cagagctgta actgataacc ttgtagctca
     2521 ctatccaaaa gcccggccag gtttattcaa tatcgggctt ctccatacaa gtttaaatgg
     2581 tcgcgaaggt catgaaccct atgcaccgtg tactctggat gaactactat ctcgtggtta
     2641 tgggtactgg gccctggggc atgttcacaa aagagaaatt gttcatcagg agccatgggt
     2701 ggtattttct ggaaactctc agggaagaaa tgcgcgtgag acaggggaaa agggctgtat
     2761 gctggtttct gtgtcggatg gcatactaac tgatatcaaa ttcataagcc ttgacatttt
     2821 ccggtggtcg gtgattgatg tggatgttac aggcttatca gagattgata gcatccttaa
     2881 cacattggat aaagccctga aacgagagct gcaggtcaat gataacatgc cacttgccct
     2941 gcgtatttgt ctgtccgggt cgtcgagact gcacgggaag attctggcca gggcagagca
     3001 tctgaagaat gagatcaggg gatgcgcaat cgatgtgggt tatggaaaca tctgggttga
     3061 aaaagtttgc atcgagactt caggaatgtc tgatcctgaa acattaatga tgagagacga
     3121 tgcgcttggt ggacttttgc gttctattag cgatctgcaa tatgatggaa aatcgcttga
     3181 cgatatgctc ggggagcttg gggatctttt tcgcaagctg ccggctgaat atttggatga
     3241 tatggaggcc attcatccgg aacggaaga
//
LOCUS       DAOSRI010000200         3231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_26871_length_3231_cov_4.535894, whole genome shotgun sequence.
ACCESSION   DAOSRI010000200 DAOSRI010000000
VERSION     DAOSRI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26871_length_3231_cov_4.535894"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3054)
                     /locus_tag="PK717_06360"
     CDS             complement(<1..3054)
                     /locus_tag="PK717_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOR47316.1"
                     /translation="MRSINNSIFGKKFLASKIKEESSISSFLKENRENLLKPIKKWLS
                     MLDSGVIKDEESFQDPFIDTIFRQTLGYKRQGDSPTWECTREYKMQGGKSSDAALGFF
                     ENGKDEHSSVFAVIELKAPFTKDLSRKPASRELSAVEQGFDYSAKIGSTCHWVIVSNM
                     DEIRLYHVGDQNRYESFNIAEIANSPTEMERFYFLLCRQNLLPYAPSSKESYLDKLYA
                     RNQADQAEINNKFYAIYSRTRQDIFNKLSETNPDIDKLVLLEKVQKILDRFLFMCFCE
                     DKGFINRSAADEELTKFILNAPKKSFRGSIWNEFMDISRAIDEGSDQPIKIPPYDGGL
                     FAKDSELENLNVSNDALESLRPIAEYDFDSELDVNILGHIFEQSVSDIEELKAKIKGQ
                     NFDEKKGKRKKQGIYYTPAYITSFIIEETIGAWIEREKIRLGFESLPQITEDEERLLS
                     EVEINPKIKIPKSLETKSKEHAKAWTGLQERLKQIKVLDPACGSGAFLNKAFDYLVSS
                     HEKIASQRKHFGDRPNTGVFGTIDQSKFDDFVAKNNLFGVDLSKESVEITRLSLWLRL
                     VYHKEKLPSLDHAIKHGDSIVADPAYTKVPFNWVTEFHNIFGGDKFSLASDTSGFDIV
                     IGNPPYVRQEFLSPTKPALQKEYQEFYNGTADLYTYFYRRGFQLLKKDGYLGFITSST
                     FTKTSSGANLRRFLKNETAIDRFVDFGLLQLFDEATNLACIVIARNTKLMPDHKIQSS
                     KIESLPGLETENLLEKAGHVEVAQNSLGEDSWNFDRPEVEALRRKIFSKGRPLKDVAG
                     SPLYGIKTGLNEAFVIDKATRDRLVKEEFGQRSKQPHPDPLLVGEGESPSCGEDAVSA
                     NNSPSTYGRNAFSPNCSPSPFGEEKDGSSCSPSPCEKGKDELSCSPSPFGEEKDGSSC
                     SSYSTSPFGRGEGEGVFGDGLSSTNHPLIKPFLVGRDLKQWHYDFRERYLVAIQKGWT
                     KQWAGKEDITEAKAWEVFEKHYPAVANCLKPFEERAKKRQDKGDFW"
BASE COUNT          806 a          687 c          690 g         1048 t
ORIGIN      
        1 ccaaaaatca cctttgtcct gtctcttttt tgctctttcc tcaaaaggtt tcaggcagtt
       61 tgcaacagcc ggataatgtt tttcgaaaac ttcccaagct tttgcttcgg tgatgtcttc
      121 tttcccagcc cactgctttg tccagccctt ctggatggca acaagatacc tctccctgaa
      181 atcgtaatgc cactgtttca ggtcgcgtcc aacaaggaat ggtttgatta gtgggtggtt
      241 tgtggaggag aggccgtcac cgaaaacccc ctcaccctcc cctctcccaa agggagaggt
      301 agaataagaa gaacaagaag atccgtcctt ttcttcccca aagggagagg gagaacaaga
      361 aagctcatcc tttcctttct cacagggaga gggagaacaa gaagatccgt ccttttcttc
      421 cccaaaggga gagggagaac aatttggaga aaaggcattc ctgccatagg tggagggaga
      481 attgtttgcg gaaacagcat cttccccaca agaaggagac tctccctctc ctaccaggag
      541 agggtcgggg tgaggttgtt tactcctctg cccaaattcc tcttttacca gcctgtctct
      601 tgtcgctttg tcaatgacaa aagcttcatt caaaccagtt tttattccat aaagagggct
      661 gcctgcaaca tcttttagtg gtctgccttt tgagaatatc tttcttctta gcgcctcaac
      721 ctctggcctg tcaaaattcc agctgtcctc accaagggag ttttgtgcca cttcgacatg
      781 gcctgccttt tccaggagat tctcggtttc cagccccggc agtgactcga tttttgagga
      841 ctggattttg tgatctggca tcagttttgt gttcctggct attactatac aagcaagatt
      901 tgttgcttca tcaaaaagct ggagcaatcc gaaatcgaca aaccggtcga ttgctgtttc
      961 atttttcagg aaacggcgaa ggttggcccc ggagcttgtt tttgtgaatg tactggatgt
     1021 gatgaaacca agatacccat cttttttgag gagctgaaaa cccctgcgat aaaaataggt
     1081 gtaaagatcg gcggtcccat tgtagaattc ctgatattct ttttggagtg ctggttttgt
     1141 ggggctgagg aattcttgcc tgacgtatgg tggattgcca atgactatgt cgaaaccgga
     1201 ggtgtcactg gcaaggctga atttgtctcc cccaaaaata ttgtggaact cggttaccca
     1261 gttgaaaggc acttttgtgt atgccggatc agcgacaatt gagtctccat gcttgattgc
     1321 atggtcaaga gacggcagtt tttccttgtg gtagacgagc ctcagccata aggaaagcct
     1381 tgttatttct acggactctt ttgaaaggtc cacaccaaaa aggttgtttt tggcaacaaa
     1441 atcatcaaat tttgactggt caatcgtgcc gaagacgcca gtgtttggac gatcgccaaa
     1501 atgttttctc tggctggcta ttttctcatg cgatgaaacc agatagtcaa aagctttatt
     1561 gagaaaagca cctgatccac aggcagggtc cagtactttt atctgtttga gcctttcctg
     1621 gaggccagtc catgcttttg cgtgttcctt tgattttgtc tcgagcgatt ttggtatctt
     1681 tatttttggg tttatttcaa cttcggaaag caatctctct tcatcttcgg taatttgtgg
     1741 caagctttca aaaccaagcc ttattttttc gcgctcgatc caggcgccta tggtttcttc
     1801 tataatgaaa ctcgtaatgt aggctggtgt gtagtaaatt ccttgttttt ttcttttgcc
     1861 ttttttttcg tcaaaatttt gcccttttat ctttgccttt aattcttcaa tatcagaaac
     1921 cgactgttcg aaaatatggc caaggatatt gacatcaagc tctgagtcaa aatcgtactc
     1981 ggcaattggc ctgagtgatt ccagggcgtc gtttgagaca ttaagatttt caagctcgga
     2041 gtctttggca aacaagccac catcataggg agggattttt attggttgat ccgatccttc
     2101 atctatggct cttgaaatat ccataaattc attccagatt gatccgcgaa aacttttttt
     2161 tggggcattg agaataaatt tggttaattc ctcgtcagcc gcagagcgat tgatgaatcc
     2221 tttgtcctcg caaaagcaca taaacaagaa acgatcaagt atcttctgga ctttttcaag
     2281 caaaacaagt ttgtcgatat ctggatttgt ttcggagagt ttattgaata tatcttgcct
     2341 tgttctgcta tagattgcat aaaacttgtt atttatttca gcttgatcgg cctgatttct
     2401 tgcataaagc ttgtcaagat aggattcttt ggatgatggt gcatatggca ggaggttttg
     2461 gcgacaaaga agaaaataaa acctctccat ttctgttggg gaattagcaa tctcggcaat
     2521 attgaatgac tcatatctgt tttgatcccc aacatggtag agtcttatct catccatgtt
     2581 cgaaacaatt acccagtgac aagtcgaacc aatttttgca gagtagtcaa atccttgctc
     2641 gacagctgac aattcccttg aggctggttt ccttgagagg tcttttgtga aaggggcctt
     2701 gagttctata acggcaaaaa cagagctgtg ttcatctttg ccattttcaa aaaatccgag
     2761 cgcggcatcg gaggattttc caccctgcat tttatattct cttgtgcact cccatgttgg
     2821 agaatcgcct tgtcttttgt atccaagtgt ttgtctgaag atggtgtcta tgaaaggatc
     2881 ctgaaaactt tcttcatcct tgattacccc ggaatcaagc attgaaagcc attttttaat
     2941 tggtttgagg agattttctc tgttttcttt tagaaaagag cttattgacg attcttcttt
     3001 tattttgcta gcaagaaatt ttttgccgaa aatgctgttg tttatgcttc tcatttttat
     3061 atagcaatat acatcttatc cagagttttg ttaagtagtc gcatacaatt acattttgta
     3121 ttcctcatcc atgttaaaca gtgtaaacga gagaatatct gtcaattgat ccacaacctg
     3181 ttttctcgaa gatgccccgt gaccgtactt cttgccaatt cttgtgagaa t
//
LOCUS       DAOSRI010000201         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_26938_length_3225_cov_3.828391, whole genome shotgun sequence.
ACCESSION   DAOSRI010000201 DAOSRI010000000
VERSION     DAOSRI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26938_length_3225_cov_3.828391"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK717_06365"
     CDS             complement(<1..1140)
                     /locus_tag="PK717_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR47317.1"
                     /translation="MKKYLTLTLALLMVLVSFGNVGFALQKSDPVHQSRMAYVSPGII
                     AQMDEGSIDDPIDAMVWTSTPIDKSLERMGQNEGLLRFEPFKFMSCYYVSATSQALKT
                     IASMPETLRVQENYSIKAPDTKMADTKNTKVNWGLDDLKVRDLWKQGIRGQGVKVGLL
                     DTGVDAKHPELAGKVTKFATFDTSGKVIPDMTEPFDNVGHGTHCAGIIAGGKSGQDGV
                     GVAPDCELIVGSVIPGGNGTLAQVIGGMEWILDPDGDPNTDDAPRVVSMSLGGSIPDE
                     DMVAATDAFERKGVLLIASIGNSGDGSTGSPGNIPSVTSVGAYDKNRQPASFSSGNKI
                     DWEIEPYHESVIKPDVSAPGVSIYSTFPKGKYAIASGTSMATPHVA"
     gene            complement(1193..2281)
                     /gene="hflX"
                     /locus_tag="PK717_06370"
     CDS             complement(1193..2281)
                     /gene="hflX"
                     /locus_tag="PK717_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOR47318.1"
                     /translation="MSKVFIVGLRMKEDDQHEALESFSELMQLARTLDMEIVGKGFQV
                     IREPTHSHLLGKGKVEELAQEIASLDPERVLVDFELSPLQCRNLEESWGRPVLDRHGL
                     IIEVFSKHATTSEGKLQVELALLKYKLPRLSGRNLAYDQQTGGGGSAYLRGSGERAID
                     IARRQIRSRIRVLEKKIELVARHREIKSKRREKSDVPLVSLVGYTNAGKSTLLNSLTG
                     ANVLTQNKLFSTLDTTVRARIHDGQKILFADTVGFIRNLPVDLVAAFRSTLEEVLSSD
                     VILHVFDATNPDWERHIEVAQGILDDLGASNIPMLYVANKSDLVSEEKQKEISSHLDD
                     VVFVSALERRGLDILLGKLSQNLFALKV"
     gene            complement(2278..>3225)
                     /gene="ispG"
                     /locus_tag="PK717_06375"
                     /gene_synonym="gcpE"
     CDS             complement(2278..>3225)
                     /gene="ispG"
                     /locus_tag="PK717_06375"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOR47319.1"
                     /translation="KTDTRDAKKTIEQIWRVYGAGCEIVRCAIPDMEAAKALAEIVKG
                     SPIPVVADIHFDYRLALASLEAGCHKLRLNPGNIKDKEKIRLVAREAKARNVPIRVGV
                     NAGSIKHTDNVSNEAVQIALEEVKILEDEDFRDIVVSVKTSDIKTTIEANRLLRKACD
                     YPIHIGLTESGPLIQGLVRSSAALLPLLEEGTGDTIRVSLTDDPESEVEAAWSMLETV
                     GVRSFGPTIISCPTCGRTRQNLRGKLSEIRMALKGVKGKKIAVMGCEVNGPGEAKDAD
                     MGIAFGATKCVVFRGGNMIGTFDTDEAVRILIEMAKEDN"
BASE COUNT          736 a          847 c          699 g          943 t
ORIGIN      
        1 agccacatgg ggtgtggcca ttgatgttcc gcttgctatt gcatacttgc cttttgggaa
       61 ggttgagtat attgacacac caggtgcaga aacatctggt tttattaccg attcatggta
      121 tggctctatt tcccagtcga tcttgttgcc gcttgagaaa cttgctggct gacgattttt
      181 gtcataggca ccaacagatg tgaccgatgg tatattgcct ggtgagccag ttgaaccatc
      241 tccactgttg ccaatggagg cgataaggag aacacccttt ctctcaaatg catccgtagc
      301 agcaaccatg tcttcatctg gaatcgaacc gccgagggac attgaaacca cccttggggc
      361 atcatcggtg tttggatctc cgtccggatc aaggatccat tccatgccgc caataacctg
      421 cgcaagtgtt ccgttcccgc cagggattac cgaaccaact atgagttcgc agtcaggggc
      481 aacgccaacg ccgtcctggc cactcttccc accagcgatt attccagcac aatgggtacc
      541 atgaccaaca ttgtcaaaag gttcggtcat atctggtata accttgccag acgtgtcaaa
      601 cgttgcgaac tttgtgacct tgcctgccaa ctctgggtgc tttgcatcaa caccagtatc
      661 aagcaaacca accttgacac cctggcccct gatgccctgc ttccagagat ccctaacctt
      721 taggtcgtcg aggccccaat tgacttttgt attctttgtg tcagccatct tggtatctgg
      781 tgcttttata gaataattct cctggaccct cagcgtttca ggcatggatg cgatggtttt
      841 tagggcctgg ctggtcgcgc tgacataata gcaggacatg aacttgaatg gttcaaatct
      901 caggaggcct tcattctggc ccatcctctc caatgatttg tctatcggtg ttgatgtcca
      961 caccatggca tctattggat catcgattga tccttcgtcc atctgtgcga tgatgcccgg
     1021 tgaaacatag gccatccttg actgatggac cggatcggat ttctgcaagg caaaaccaac
     1081 attaccaaac gatacgagaa ccatcagtaa tgctagtgtc aaagtcaaat attttttcat
     1141 ctaaccactc cttttcgggt cattatttta caacgcatgg attttgttct ggctaaactt
     1201 tgagggcaaa aaggttttgt gataatttgc ccagaagtat atcaagcccc cttctttcaa
     1261 gggccgaaac aaaaaccacg tcatcaagat gacttgatat ttctttttgt ttttcttcgg
     1321 atacaaggtc gctcttgttg gcaacataca gcattgggat gttgcttgct ccaagatcat
     1381 cgaggatgcc ttgtgctact tcaatgtgac gttcccaatc agggtttgtg gcatcaaaca
     1441 catgcagtat gacatctgaa gacagcacct cttcaagtgt tgacctgaat gctgcaacga
     1501 gatcgacagg gaggtttctt ataaaaccaa cggtatctgc gaacagtatt ttttgcccat
     1561 catgaattct cgccctgaca gttgtgtcaa gcgttgaaaa gagtttgttc tgtgtgagga
     1621 cattcgcccc tgtaagactg ttcaggagtg ttgacttgcc agcattggtg tatccaacaa
     1681 gtgaaactag cggaacatca ctcttttcgc gtcttttgga ctttatttcc ctatgtctgg
     1741 ccaccagttc tatcttcttc tcgagcaccc tgattctgga gcggatctgc cttcttgcaa
     1801 tatctattgc cctttcacca gaacccctaa ggtatgcaga acctccacca cccgtttgct
     1861 ggtcataggc aagatttctg ccagacaacc ttgggagttt atatttaagg agtgcaagtt
     1921 ccacctgaag ttttccttca gatgtggtgg cgtgctttga aaacacctca attatcaggc
     1981 catgcctgtc gaggaccggc cttccccatg actcttccag gttcctgcac tggagtggcg
     2041 agagctcaaa atcaacaagg actctctctg ggtctagaga cgctatttcc tgcgccagtt
     2101 cttcaacttt gcccttgccc agaaggtgcg aatgggtggg ttccctgatt acctgaaaac
     2161 cttttccaac tatttccatg tccagcgtcc tggcaagttg catcagttcg ctaaatgatt
     2221 caagagcctc gtgctgatcg tcttctttca ttctgaggcc cactataaaa accttactca
     2281 attatcctcc ttggccattt ctatcaatat ccttacggcc tcgtcggtgt caaaagttcc
     2341 aatcatgttg ccacctctga aaacaacaca ttttgttgct ccaaaggcaa tccccatatc
     2401 agcgtccttg gcttcaccag gcccattcac ctcgcagccc atgacagcta tttttttacc
     2461 tttgacccct tttagtgcca tacggatctc cgacagcttc ccgcgcaggt tttgccttgt
     2521 ccttccgcag gtggggcagg atattattgt tggcccaaac gacctgacac ccacagtctc
     2581 gagcattgac cacgccgctt ccacttctga ttccgggtca tcggtcagcg agacccttat
     2641 cgtgtccccc gtgccttctt caagcagggg caaaagtgcg gcgcttgacc tcacgaggcc
     2701 ctgtatcaat gggccagatt cggtcaagcc tatgtgtatc gggtagtcac atgcttttct
     2761 caaaagacgg tttgcctcaa tggtcgtttt tatgtcggat gtttttacag atacaacaat
     2821 gtctctaaaa tcctcatctt caagtatttt tacctcctcc aatgcaattt gaaccgcttc
     2881 attggacaca ttgtccgtgt gtttgattga tccagcattg acaccgaccc ttatgggaac
     2941 atttctggcc ttggcttctc ttgcaacaag ccttattttt tctttgtcct ttatattgcc
     3001 agggttgagc ctgagcttgt ggcaaccggc ttcaagagat gcaagcgcca gtctgtaatc
     3061 aaaatggatg tctgcaacaa cagggatggg gcttcctttt acaatttcgg caagggcctt
     3121 tgcagcttcc atgtcgggaa tggcgcacct gactatttca cagccagccc catatacacg
     3181 ccatatctgt tcgattgttt tcttcgcgtc cctggtgtcg gtctt
//
LOCUS       DAOSRI010000202         3223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_26958_length_3223_cov_3.760732, whole genome shotgun sequence.
ACCESSION   DAOSRI010000202 DAOSRI010000000
VERSION     DAOSRI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26958_length_3223_cov_3.760732"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK717_06380"
     CDS             <1..648
                     /locus_tag="PK717_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47320.1"
                     /translation="AINYALYLHQNDEKVALLDFDVVKPYIRIRDKADILRKAGLEVL
                     MPEGSIAFADMPIIPTHLYAWVADKSRKLVVDVGGDKQGSTALAQVMPRIETNGYEFI
                     LVVNPFRPFSKTVDQIKTIAGEVAFGARAKFTHIVANPHLKEKSTRKDFLEGLEIVKQ
                     ASKEMELPIKFVAVSHEYLKDVTQDDLPAPMLPLQLFIKYPWEEKPRVNWIYRRG"
     gene            complement(645..1838)
                     /locus_tag="PK717_06385"
     CDS             complement(645..1838)
                     /locus_tag="PK717_06385"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOR47321.1"
                     /translation="MNGKSAVLLIIDGLGVGCLPDAAQYEDAGTSTLSHATENGKLNL
                     PYLTKFGLYKLCGRDGPSQASTGRMVPKSSGKSTVEGHWEMMGQPKTNPFNVFPDGFS
                     SEIMDTISKLIGRGLLGGQPASGTKIIEQLGERHQKTGMPIIYTSADSVLQVAAHENT
                     IPVNELYEICKTIRNNFNQPDSFARVIARPFAGEPGNYYRTSRRRDFTLKIPEPNVLS
                     ELKKRNINISGVGRIADLFPEYLDTFDNPPGNIECLERTKMYRQMYHGFVFANLEDLD
                     MLYGHRRDKQGYAKSLAELDEAWPKFLMSLANDDLLIVASDHGCDPTHEKHTDHTREY
                     TMLMCYMKGYEGVDLGTRQSLCDVGATILDFFGINPLCGESVLKDATSCKKKTKTSTN
                     FYNNN"
     gene            complement(2338..2511)
                     /locus_tag="PK717_06390"
     CDS             complement(2338..2511)
                     /locus_tag="PK717_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47322.1"
                     /translation="MSFSRTLLHQLFGVGSDAKSVSADPPVVAPAGGGGGAPPPPEGH
                     QPYGLKAVEPLVP"
     gene            2534..2806
                     /locus_tag="PK717_06395"
     CDS             2534..2806
                     /locus_tag="PK717_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47323.1"
                     /translation="MGECKGQAPVNNIEISLDGGSTWFKANLNTKTNEWSYELNTSIQ
                     GSTYQLLTRATDKAGRTERTVNPNLDVRYRKRPVTINIVSPGTGRE"
     gene            complement(2961..>3223)
                     /locus_tag="PK717_06400"
     CDS             complement(2961..>3223)
                     /locus_tag="PK717_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47324.1"
                     /translation="IAATYIDQSSPTRFMTAMRPSSANSVPILVSDESIEEAQEMLEE
                     YMAAVPDLASEFQDDEEDDFDDEDFDDEDDDEEEEYKGSQIC"
BASE COUNT          932 a          668 c          670 g          953 t
ORIGIN      
        1 gccataaatt atgctctcta tctccaccaa aacgatgaaa aagttgccct tttagatttt
       61 gatgttgtca aaccatacat acgaatcagg gataaagcgg acatcttgag aaaggctgga
      121 cttgaagtcc tgatgccgga aggatcaatt gcttttgctg atatgccaat aattccaaca
      181 cacttgtatg cgtgggtggc ggacaaatcc agaaaactcg ttgtagatgt cggtggcgac
      241 aagcagggtt caacagcact tgcgcaagtg atgccaagga tcgaaactaa tggatacgag
      301 tttatattgg tagtaaatcc ttttagacca ttttccaaaa ctgtggatca gataaagaca
      361 attgcgggtg aggttgcttt tggagcacgt gctaaattca cgcatattgt tgcgaaccca
      421 caccttaagg aaaaaagtac aagaaaagac ttccttgaag gcttggaaat agtaaagcaa
      481 gcctcaaaag aaatggaact tccaataaaa tttgtggccg tttcacacga atatctgaaa
      541 gatgttactc aggatgatct tccagctcca atgcttccat tgcaactttt tataaaatat
      601 ccttgggaag agaaaccaag agtcaattgg atatatagaa gaggttaatt gttgttgtag
      661 aaatttgttg aggtcttggt tttctttttg cagctagtgg catcctttag cacagattca
      721 ccgcacaaag ggtttatccc aaaaaagtcc aggattgttg ctccaacatc gcaaagcgat
      781 tgccttgtcc caagatcaac accctcataa cctttcatgt agcacatcaa catggtgtat
      841 tctcttgtat gatcggtgtg cttctcatgt gttgggtcac agccatgatc agacgccact
      901 attagaagat catcattggc aagagacatg aggaatttgg gccatgcctc atcaagctct
      961 gcaagtgatt ttgcataccc ctgtttgtcg cgtctgtggc catagagcat gtccaggtct
     1021 tcaagattgg caaagacgaa accatgatac atctgcctat acatttttgt tcgctcaagg
     1081 cactcaatat tgccaggtgg attatcaaaa gtatcaaggt attctgggaa aaggtcagct
     1141 atgcgtccca ccccagatat gtttatattg cgttttttga gctctgaaag gacatttggt
     1201 tccgggatct tgagtgtaaa atcccttctc ctggaggttc tgtaataatt tcctggctca
     1261 ccagcaaatg gcctggcaat taccctcgca aagctgtctg gttgattaaa gttgttcctt
     1321 attgttttgc aaatctcata aagttcgttt actggtatcg tattctcatg ggctgcaacc
     1381 tgaagtacgg aatcggctga tgtatagatt atcggcatgc cagttttttg gtgacgctct
     1441 ccaagctgtt cgatgatttt tgttcctgaa gctggttggc caccaaggag tcctctccct
     1501 atgagcttag atatagtatc catgatctct gaagaaaacc catctgggaa aacgttaaaa
     1561 ggattagttt ttggctgacc catcatctcc cagtggcctt ctactgttga tttgcctgat
     1621 gatttaggaa ccatgcgacc tgttgatgcc tggcttggtc catccctgcc acaaagttta
     1681 tacagaccaa attttgtgag atatggtaaa tttaatttgc cattttcagt tgcatgggag
     1741 agagttgacg tacctgcatc ctcgtactgt gcagcatcag ggagacaccc aactcctaga
     1801 ccatcaataa tcagcagaac agcacttttt ccgttcatat agaattatat taatgtctaa
     1861 attgattgtt ctggttaaat aagaataaac cacttccaga tatttcactt tttaccatag
     1921 ttttcttaaa atcaccttca cttctaaaat ttgtgaaatt ttgattatat atagcttagt
     1981 tctgatttga cctgataaca ggtcaacaag attcaaaatg tgaaattatt gttgaatgtt
     2041 gtcacgccat cattcaacat ggtggatttt ggactttttt acgatataat gatatattac
     2101 cgaaaaataa gcgaaaatat aaaatcattg tttgtaagag gatacagcat ttgcgtgatg
     2161 atttgtgaac ctagtacaaa actagtgtaa atatctccaa aatggaccat tgcatttaag
     2221 attgaattgt gattgaatac taacataaat tcaggcgaaa ggagccaaac atatgaaaaa
     2281 actactagtt gtttcagtac tgtttgcctt agcctgtggt ttgatgatgc cggtgaccta
     2341 tggaaccaat ggttcaacgg ctttcagacc atatggctga tgtccttctg gaggaggcgg
     2401 tgcgcctcca ccaccacccg caggtgcaac taccggtggg tcagctgata ccgatttggc
     2461 atcggatccg acgccaaaga gctggtgcag caatgttctt gagaatgaca caatcagact
     2521 tccatttaac ctaatgggcg agtgcaaggg ccaggcccca gtcaataaca tcgagatctc
     2581 gcttgacggc ggttcaacat ggttcaaggc aaatctgaac accaagacaa atgagtggtc
     2641 ctatgagctc aacacatcaa ttcagggctc aacctaccag cttttgacaa gggcaaccga
     2701 caaagcaggc aggacagaac gcacagtcaa tccaaatctt gatgtcagat atcgtaagcg
     2761 tccggtcaca atcaacattg tttcgccagg gactggaaga gagtaactca aacctagttt
     2821 ttccacaaaa aagaccagct tttaagctgg tcttttttgt ttttgggcac aaatacgaat
     2881 agcttttata attcctttga atataaaaaa gctggttttg aaaaaaccag cttttttgtt
     2941 atctgatggc tattattaaa tcagcaaatt tgagagcctt tgtactcctc ttcctcatca
     3001 tcgtcttcat cgtcaaaatc ttcatcgtca aaatcgtctt cttcgtcatc ctgaaattca
     3061 ctggcaagat ctggaactgc cgccatatat tcctccagca tctcttgcgc ctcctcaatt
     3121 gattcgtctg aaactaaaat tggaactgag ttggctgaag aaggtctcat tgcggtcatg
     3181 aaccttgttg gcgaagattg atcaatgtag gttgcagcaa tgt
//
LOCUS       DAOSRI010000203         3198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_27249_length_3198_cov_3.824372, whole genome shotgun sequence.
ACCESSION   DAOSRI010000203 DAOSRI010000000
VERSION     DAOSRI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27249_length_3198_cov_3.824372"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..>3198
                     /gene="dnaE"
                     /locus_tag="PK717_06405"
     CDS             509..>3198
                     /gene="dnaE"
                     /locus_tag="PK717_06405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR47325.1"
                     /translation="MFVHLHNHSEFSLLDGAIKVYEMAARAAKLGMPAIALTDHGTMY
                     GAIDFYKACTKQKIKPIMGCEFYVAPQNMESKETRSGMYHLVLLAENNKGYENIMRLV
                     TEANIRGFYYKPRVDYDTLAKYSEGVLALTACISGEVPEKLLLDDEEGAKKALGTYKE
                     IFNNRLWVELQDHGLKEEVSVMPKLIELAKNFELPVVATQDAHYLEHADAEAHDILLC
                     IQTLSKQDDPDRMKFANDEFYLKTPGQMQELFKWIPEAVTNTLEIADRCNVTIELGKP
                     HPPKFTDIGNDDPEAHKQFLWDLVNKGASWRFGCAISQDIRNRLDYEMRIIDQTGFVD
                     YFLVVWDFIKYAKDHDVGVGPGRGSGAASLVSYCLNITDVNPLEYGLFFERFLNPDRI
                     SPPDFDIDFDDTKRDRVIKYVTQKYGEDSVAQVATFGRMEARGVIRDVGRALGFSYGD
                     MDKIAKLIPFGAELEEALAGVPQLREIQQQNDQTKKLFEVAKRLEGVVRNFSTHAAGV
                     VMADKPLYCYVPLQLDKEGKRVTQFEKNAVEELGILKVDFLGLRNISVINNACETIGK
                     KTGEKLDIDKVPLDDQATYQTLQSGDTMGVFQLESSGMRRYLRELKPTVIQDIIAMVA
                     LYRPGPMEFIPQFIGGKHGEVVTKYLDPCLEPILEGTYGVAVYQEQVIQIARDFAGFT
                     LGQGDILRKAVAKKIPELLAEQKRLFIERASQNGKNPQIAEEVFKFIEPFAGYGFNRA
                     HSTCYGLLAYKTAYLKTHYKLEYYTAILTSYVGNEDRIKEMYEECRKSGLNVLPPDIN
                     KSEWTFEVEGEAIRYGLGALKNVGEVAVEAIIAERKSGGPFKNFEDFQKRCEGSKANK
                     KVIESLIKAGVFDAFNEDRGHLLKKFSNGGGQMAFSLFAP"
BASE COUNT          973 a          710 c          757 g          758 t
ORIGIN      
        1 aaaatattga cttttatgta aaatttttta cataaatgat tattttttaa aaaagatacc
       61 caaaactcca ccttattaca cttcatatgc accatataga acaggatcag gtgactggtt
      121 tgaattgatg tgttatattt atgaacgtga tggcatgcat aaatacaatc ttggggattt
      181 tggacgacgc attttcaaaa tcatcagcga tgacacagaa gtactcatga taacagagag
      241 aggtttggaa ttaacaattt ttggagatga ttatattaaa cctgttgaaa taatagatat
      301 gaataaatac taacaaatcg gtgacaaatt tagccaagct ttgaacgttc ctggtacccc
      361 tgaaggcaat gggtggcaga ggtctgataa tgctcctaaa acagtccagt attagagcaa
      421 tctatatttc tcaaggtttg cacatgcaca tgcaacaact taccccttct gcattgataa
      481 gtgcacctta tattgtaaaa tctaaccaat gtttgttcat ttgcataacc attccgaatt
      541 ctcgctcctg gatggcgcca taaaggtcta cgagatggcg gccagggcgg caaagttggg
      601 catgccagcc atcgcattga ccgatcacgg gacaatgtat ggtgcaattg atttctacaa
      661 ggcatgcaca aaacagaaga tcaagccgat catgggttgt gagttctatg tagcacccca
      721 aaatatggaa tcaaaagaaa ccaggagcgg gatgtaccac ctcgttcttc tcgctgaaaa
      781 caacaagggc tacgagaaca tcatgaggct tgtgacagag gccaatatca ggggtttcta
      841 ttacaagcca agggtcgatt atgatacgct ggcaaaatac tcggagggtg ttcttgctct
      901 aacggcctgc atctccggcg aggtgcctga aaaactcctg ctggacgacg aggaaggtgc
      961 caaaaaagct ctcggcacat acaaggagat ttttaacaac aggctctggg tcgaacttca
     1021 ggaccacggg ctgaaggaag aagtcagcgt aatgccgaaa ctcatcgagc tggcaaaaaa
     1081 cttcgaactg ccggttgttg caacacagga cgcccattac cttgaacacg ccgatgccga
     1141 ggcccatgac atccttctct gtattcagac gctttcgaag caggatgacc ctgacaggat
     1201 gaaattcgcc aatgacgagt tttatctcaa gacccctggg caaatgcagg agctattcaa
     1261 atggataccc gaggcggtca caaataccct ggaaatagcc gatcgatgca atgtcacaat
     1321 cgagctgggg aaaccccacc cgccaaaatt taccgatatt ggtaatgacg atccggaggc
     1381 acacaagcaa tttctttggg accttgtaaa caagggtgca agctggagat ttggttgtgc
     1441 catttcccag gacatcagga acaggctcga ttatgagatg aggataatcg accagacagg
     1501 ttttgttgac tatttcctgg tcgtctggga cttcataaaa tacgcaaaag accacgatgt
     1561 tggcgttggc ccaggcagag gctcaggcgc agcatcgctt gtttcttatt gtctcaacat
     1621 aactgatgta aaccctcttg aatacggact gttttttgag aggttcctga accccgaccg
     1681 aatctcacca cctgactttg acatcgattt tgacgataca aaaagggaca gggttataaa
     1741 gtatgtaacc caaaaatacg gcgaggattc tgttgcccag gttgccacat tcggccgaat
     1801 ggaggccagg ggagtaatca gggacgttgg aagagcgctc ggcttcagct atggtgacat
     1861 ggacaagata gcaaaactta ttccattcgg ggctgagctg gaagaggccc ttgccggtgt
     1921 tccacagcta agggaaatcc agcagcaaaa cgaccaaacc aaaaaacttt ttgaggtcgc
     1981 aaaaagactt gaaggcgtcg tgcgcaattt ctccacccat gccgcaggcg tcgtaatggc
     2041 tgacaagccc ctttactgtt atgtcccact tcagcttgat aaagaaggaa aacgcgtcac
     2101 acaattcgaa aaaaatgccg ttgaggagct tggaatacta aaggtagact tcctcggcct
     2161 aagaaatatt tctgtaataa acaacgcctg cgaaacgata ggcaaaaaaa ctggagaaaa
     2221 actggacatt gacaaggttc cgcttgatga tcaggccacc tatcaaacgc tccagtctgg
     2281 cgacacgatg ggtgttttcc agcttgaatc ctctggtatg aggcgctacc tgagggagct
     2341 caaaccaacc gtaatacagg acatcatagc catggtggcg ctctacagac cgggcccgat
     2401 ggagttcata ccccaattca tcggaggtaa gcatggtgaa gttgttacaa aatatcttga
     2461 tccgtgcctt gagccaatcc ttgaggggac ctacggcgtg gccgtatatc aagaacaggt
     2521 catccagata gcaagggatt ttgcaggttt cacacttggc cagggtgata ttctcagaaa
     2581 agctgttgca aaaaaaatcc ctgagctcct tgccgaacaa aagcgccttt ttatcgagag
     2641 ggcgtcccag aacggcaaaa atcctcaaat agcagaagag gtgtttaaat tcatcgagcc
     2701 ttttgcagga tacggtttta acagggctca ttcgacatgc tacgggctgc ttgcatataa
     2761 aacagcctat ctaaaaacac attacaaact tgaatattac acggctattc tcacctcata
     2821 tgtcggtaac gaagacagaa tcaaggaaat gtatgaggaa tgcaggaaat cgggcctcaa
     2881 tgttcttcca cccgacataa acaaatctga atggacattt gaagtcgaag gagaagcaat
     2941 caggtatggg ctcggtgccc ttaaaaatgt cggtgaagtg gccgtcgagg ccatcattgc
     3001 cgaaagaaaa tctggcggtc ctttcaagaa ctttgaggac ttccagaaga ggtgcgaagg
     3061 aagcaaggcc aacaaaaagg tcattgagag tctcataaaa gcgggcgtat ttgacgcctt
     3121 caacgaagac aggggccatc tgttaaaaaa attctcaaac ggtggcgggc aaatggcatt
     3181 ctcgcttttc gccccaca
//
LOCUS       DAOSRI010000204         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_27401_length_3186_cov_4.227403, whole genome shotgun sequence.
ACCESSION   DAOSRI010000204 DAOSRI010000000
VERSION     DAOSRI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27401_length_3186_cov_4.227403"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK717_06410"
     CDS             <1..426
                     /locus_tag="PK717_06410"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOR47326.1"
                     /translation="GCGVQVFGVPLVNMAQGFARFSNPESAPLDLQEPAKMVFKAMTK
                     NQELVAGIDRVDTIAMKDNPDFAIKSGAEGVNCISANKKGLALKMESGEGHEPFYCVV
                     ANCVRLLGGKIGELEMFNNQPLMSTNGAQSGQVVWRGPF"
     gene            complement(423..1970)
                     /gene="rho"
                     /locus_tag="PK717_06415"
     CDS             complement(423..1970)
                     /gene="rho"
                     /locus_tag="PK717_06415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOR47327.1"
                     /translation="MEDLVRVYTAEELEDKTARQLYDICKALKIKNYTSFKKDDLINE
                     IVLVSRGEKVIVRKKRGRKSKAELEAMRKAAEQASKTEVKKETLFTPTQQPEKKYEYK
                     TQEQTYKTSEQSQYRKPEYSQTFSSEPQSETPPRGDQSMGQGGGVLEIHPDGYGFLRL
                     NYVPNPKDIYLAPSQIRRFGLRNGDFVEGSVRYPTKPGEKYAAMLKLDSVNGMDPEYI
                     RNRPHFENLIPIFPDERIKLELRGVNDTTLRLIELTSPIGKGQRGMIVSPPKVGKTTI
                     IKKIAKAISINSPDTVLMILLVDERPEEVTDMQRSVRAQIISSTFDQLPENHIRVVEL
                     AIERAKRLVEMKKDVVVLMDGITRLTRAYNLVMSPTGRTLTGGLDTSAIHGPKKILGA
                     ARNIDNGGSLTILATCLVETGSKMDEVIYEEFKGTGNMELVLDRKLAERGLFPAIDVK
                     RSGTRRDELLYGEEERKRIWSLKRFLSNLENYEALEKILKWLSDTHNNEQFLRSIVPE
                     ENPKGRV"
     gene            complement(1958..2368)
                     /locus_tag="PK717_06420"
     CDS             complement(1958..2368)
                     /locus_tag="PK717_06420"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOR47328.1"
                     /translation="MEVFTDGASRGNPGKASFGVVITVDGDTVIEESHEIGIKTNNTA
                     EYAGLIFALSMLLALKLEKEQITLNSDSEFMVKQIHGQYRLKKEELAEYYLVAKNLLM
                     RFPKIEIKHVKREKNAKADKLANLALDEGGLWKI"
     gene            complement(2374..3054)
                     /locus_tag="PK717_06425"
     CDS             complement(2374..3054)
                     /locus_tag="PK717_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47329.1"
                     /translation="MIDWQIRSTQNEIDTLDDGRACKKELEVINQKLSLALAKKKQIS
                     SLLDDLKFKYDKALAEKKKMETQLFSQGVSPKTISEIQTRLASINSHIDEVETGQLEA
                     MEAFEKITASCDKLQAMSDQKTQELETITQKFKNMTVKGQIKLKELGLERDSIRKVID
                     PSLLTKYDRLAEQKMGLAMVEVINNLCTGCQQPISITLCDSIKSEPEEIHYCNNCGRM
                     VFIRRTQI"
     gene            complement(3102..3175)
                     /locus_tag="PK717_06430"
     tRNA            complement(3102..3175)
                     /locus_tag="PK717_06430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3139..3141),aa:Ile,seq:cat)
BASE COUNT          670 a          726 c          735 g         1055 t
ORIGIN      
        1 ggttgtggtg tccaagtttt tggtgtgccg ctcgtcaata tggctcaggg ttttgcgaga
       61 ttttccaatc cggaatctgc accactcgat ctccaggaac ctgcaaaaat ggtgtttaag
      121 gcgatgacaa agaaccagga acttgttgcg ggcattgaca gggtcgacac tattgccatg
      181 aaagacaatc ccgattttgc catcaaatcg ggtgcagaag gagtcaattg catctcggca
      241 aataaaaaag gccttgccct caagatggaa tcgggcgaag gccatgagcc attttattgt
      301 gttgttgcta attgtgtccg cctgcttggt ggaaaaatcg gcgagcttga gatgtttaac
      361 aatcaacccc taatgagtac aaatggggcc cagtcagggc aagttgtctg gagagggcct
      421 ttttagaccc ttcccttcgg gttctcctcc ggcactatgc tcctgaggaa ctgctcgttg
      481 ttgtgtgtgt cgctgagcca cttgagtatc ttttcaagcg cctcataatt ttcgaggttg
      541 gagaggaacc tcttgaggct ccatattctc tttctttcct cttcgccata aagcaattca
      601 tctcttcttg ttcctgatct cttgacgtct atggcaggga agaggcctct ttctgcaagc
      661 tttcgatcga ggacgagctc catgttacct gtacccttga attcttcgta tatgacctcg
      721 tccatttttg aaccggtttc aacaaggcat gtggccagga tggtcagtga gccaccattg
      781 tcaatgttcc ttgcggctcc gaggatcttt tttggtccgt ggattgctga cgtatcaagt
      841 ccaccggtga gcgtccttcc agttgggctc atgacaaggt tgtacgccct tgttagcctt
      901 gtaataccat ccatgaggac aacaacatcc ttcttcattt cgacaaggcg ttttgccctt
      961 tcaattgcaa gctcgacgac cctgatgtgg ttttccggga gctggtcgaa ggttgatgat
     1021 atgatctgtg cccttacgga cctttgcatg tctgttactt cctctggacg ctcgtcaaca
     1081 aggaggatca tgaggacggt atcgggactg ttaatcgaga ttgctttcgc aatctttttg
     1141 atgatggtgg tttttccaac ttttggtggt gaaactatca tgccacgctg gcccttgcct
     1201 atcggggaag tcaactcgat gagtctaagt gtggtgtcgt ttacgcccct gagctccagc
     1261 tttatcctct catcaggaaa aatcggaatg aggttttcaa aatggggtct gttgcgtatg
     1321 tattcggggt ccatcccgtt taccgagtca agctttaaca ttgcagcata tttctcgccg
     1381 ggttttgttg ggtatcttac agagccttct acaaaatcac cattcctgag accaaaacgc
     1441 cttatttgtg aaggtgcaag gtaaatatcc ttggggtttg gcacgtagtt gagcctgagg
     1501 aaaccatacc catcaggatg tatctccaga actccgccgc cctggcccat tgactggtcg
     1561 ccccttggcg gtgtttcgct ttgtggttcg ctcgaaaaag tttggctgta ttcaggttta
     1621 cgatactgcg actgttcgct ggttttgtat gtttgttcct gtgtcttgta ttcgtatttt
     1681 ttttctggct gttgggtcgg cgtaaaaagc gtttctttct tgacttcggt ttttgatgcc
     1741 tgttcagcag cttttctcat cgcttcgagt tctgctttgg attttctgcc tctttttttg
     1801 cggactatga ctttctcgcc tcttgagaca agaacgatct cgtttataag atcgtctttt
     1861 ttgaagctcg tgtagttctt gattttgagg gccttgcaaa tgtcataaag ttgtcttgca
     1921 gttttgtcct caagctcttc ggctgtatat actctaacta aatcttccat agtcctcctt
     1981 catcaagcgc caggttggca agtttgtcag ccttggcgtt tttctcacgt ttaacgtgtt
     2041 tgatctctat ttttggaaac ctcatcaaga ggtttttcgc aaccaggtaa tattcggcca
     2101 gttcttcttt tttaagcctg tactggccgt gtatctgttt gaccatgaat tcagaatctg
     2161 aatttaatgt tatctgttcc ttttccagtt ttagcgccag aagcatcgac agtgcaaaaa
     2221 tgagcccggc gtattcagcg gtattgtttg ttttgatacc tatttcgtgt gattcttcaa
     2281 tgacagtgtc cccatccaca gtaattacaa ccccgaacga agcctttcct ggattgcctc
     2341 tcgatgcccc atcagtaaaa acttccactt ttttcaaatc tgggtcctcc tgataaaaac
     2401 catccttccg caattgttac aatagtggat ttcttccggt tccgatttta tcgagtcgca
     2461 cagtgtaatg gagattggtt gctgacaacc tgtacaaagg ttgttgatta cttcaaccat
     2521 tgcgaggccc attttttgtt cagcaagcct gtcatacttg gtaagaaggg aaggatctat
     2581 gactttccgt attgaatctc tttccagacc aagctctttg agtttaatct ggcctttaac
     2641 ggtcatattt ttgaattttt gggttatagt ttccagttcc tgggttttct ggtctgacat
     2701 tgcctgcagt ttatcacagg aagcagtgat tttttcaaaa gcttccattg cctcgagctg
     2761 tcctgtttca acttcgtcta tgtgggagtt tatggatgca agccttgtct gtatttcaga
     2821 tattgttttt ggcgaaacac cctgcgagaa gagctgggtc tccattttct ttttttcggc
     2881 cagggccttg tcatatttga atttcaaatc atcaagcagc gacgatattt gtttcttctt
     2941 tgctagtgcc agggaaagtt tctgattgat aacttccaat tcttttttgc aggcccttcc
     3001 gtcatcaagt gtgtctattt cgttctgggt ggaccggatt tgccaatcaa tcaaagacag
     3061 ttggacgagt tcggaaaatc cttgcatgtt ttcttggagt ctgggaccag taggactcga
     3121 acctacgacc cgctggttat gagccagctg ctctgaccga ctgagctatg gtcccgtacg
     3181 accgtg
//
LOCUS       DAOSRI010000205         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_27552_length_3171_cov_3.785302, whole genome shotgun sequence.
ACCESSION   DAOSRI010000205 DAOSRI010000000
VERSION     DAOSRI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27552_length_3171_cov_3.785302"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK717_06435"
     CDS             complement(<1..599)
                     /locus_tag="PK717_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR47330.1"
                     /translation="MKKIIVLAFVAMMVFGPVVNVLAVDGDPSAVDTIQLGDPKVSET
                     VNEPDFFSSIAKFFDTLIPMVPWALLASVIFIAMWVYFDASRRTNYGWLWGIASLLVV
                     PWLIYLACRPPYTIEEMKLIEADANLRRIEKEYYQYVLTKEKYICGVCGTPVQPDYQV
                     CPSCYKELKKVCPSCGKLLDPEWHICPYCASRITDRPKGA"
     gene            complement(634..1944)
                     /locus_tag="PK717_06440"
     CDS             complement(634..1944)
                     /locus_tag="PK717_06440"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOR47331.1"
                     /translation="MNYCEALDKLGSLHYLGSRLGLGRMIPLLESVGSPQYCYPYIHV
                     TGTKGKGSTSLMISNIMQASGYKVGLYTSPHLQSLRERIRVNGELITTGEFSDLVDTV
                     SQVAEGLDSELGPATFFEVMTAMAMLHFKNAGTHVGIFEVGLGGRLDATNVVEKPACS
                     VITAISYDHMGILGDTLERIAKEKAGIIKKNCPLICAKQPAKALETLLGIAKDRNATV
                     SIQGKDFDYIDRGAANGIGQTMDYHSDFGTIKDIFVPLLGEHQLQNASIAIRAAEVTS
                     RNVGLKISEDAIKEGIKNSFWPCRLEVFPGEPTIILDGAHNGASADALAHALRRHFKY
                     NSLTMVVGLLRDKDTTRFIRALAPMANKIIATTPSYYRKLSSDKLCMRFRRRLFECDS
                     IDNPADAVVKAIESTPKGGAVLVTGSLYLVGEMRTLLGQFALAK"
     gene            complement(1946..>3171)
                     /locus_tag="PK717_06445"
     CDS             complement(1946..>3171)
                     /locus_tag="PK717_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR47332.1"
                     /translation="PDKTEDFEYYYPGSLLLTAYDIIFLWVARMIFSGLELVGKKPFD
                     DVYLTGLIRDEHGRKMSKSLANAIDPIEVIDKYGADALRFTLTYLSTLGGQDISLGDK
                     ALTEGRNFINKVWNASRFTIMNLEGYTPGPIENPTFADRWIISKLSETVREVTASFDC
                     FDFAKAARTIYDFFWTDFCDWYVEMSKLPLQSGGKDKERTQRVLKLVLDNTLRILHPI
                     APFVTEEIWQKLPHEGKALIVAAWPKPEDLTFDKQAMEEMEVLQGAVKSIRNLKASLR
                     VHQAIVPCSFMAKGNDAIVLEREKDFVIKLARIEGFNLVQSQPEGTVVAVFKDMKFFL
                     DLSGRIDVVEEKKRVAGSIEKTRAEMDKIQALLSNPDFAKRAKPEVVEKNRARQEELS
                     AELSALNELALSLGA"
BASE COUNT          730 a          763 c          748 g          930 t
ORIGIN      
        1 gctccctttg gcctgtccgt tattctcgaa gcgcaatagg ggcaaatatg ccattccgga
       61 tcaaggagtt taccgcagga tggacagact ttcttgagtt ccttgtagca gcttgggcag
      121 acctgataat cgggttgtac cggtgttccg caaacgccac agatgtattt ctcttttgtg
      181 agaacgtact ggtaatactc tttttctatt cttcttaggt ttgcatctgc ctcgataagt
      241 ttcatttctt caatggtata aggcggcctg caggcaagat aaatgagcca tggtacgaca
      301 agcagggatg cgatgcccca taaccagccg taattcgtgc gtctggacgc atcaaaatat
      361 acccacatgg caataaaaat caccgaggca aggagtgccc atggaaccat cgggatgagt
      421 gtgtcaaaga atttggcaat cgatgagaaa aagtctggtt catttacggt ttcagatacc
      481 tttggatcac caagctgtat tgtatcgaca gcagaaggat caccatccac tgcaagaaca
      541 tttacgacgg gtccgaagac catcatggcc acgaacgcca gaacgataat ttttttcaca
      601 atgtcctcca agcaaagatt attgattatt ctgttatttt gcaagtgcaa actggcctaa
      661 aagtgttctc atttcaccaa caaggtatag cgagcctgtc actagtactg cgccaccctt
      721 tggagttgat tcgatggctt ttactaccgc atcggccggg ttgtctattg aatcgcattc
      781 gaacaatctt cgcctgaaac gcatacacag tttgtcagag gagagtttcc tgtagtaact
      841 tggtgtggtt gcaatgattt tgttggccat tggggccaga gcccttatga accttgtggt
      901 gtccttgtcc ctgaggagtc cgaccaccat tgtcagcgag ttgtatttga agtgacgcct
      961 aagggcatga gcaagtgcat cggcagatgc accattgtgg gcgccatcca ggatgatcgt
     1021 tggctctcct gggaacactt ccagtctgca tggccagaaa gagtttttta tgccttcttt
     1081 tattgcatct tctgatattt ttagccccac gttcctggaa gtcacttcag cggcccttat
     1141 tgcaatggag gcattttgga gctgatgttc accaagaagt ggaacaaaaa tatcttttat
     1201 ggtgccaaaa tccgagtgat agtccattgt ttgcccaatg ccattggcgg cccctctgtc
     1261 gatatagtca aaatctttcc cctgaattga aacggttgcg ttacggtctt ttgctattcc
     1321 aaggagtgtc tcgagggctt ttgccggttg ttttgcgcaa ataagtgggc agtttttctt
     1381 tataatacca gctttttcct tggcgatgcg ctcgagggtg tcgccaagta ttcccatatg
     1441 gtcatacgaa attgcagtta taactgaaca tgcaggtttt tcgacgacat ttgttgcatc
     1501 cagcctccca ccaaggccaa cctcaaagat cccaacatgt gtgccggcat ttttgaaatg
     1561 gagcattgcc atggcggtca ttacttcgaa gaaagttgct ggcccaagtt cgctatcaag
     1621 cccttctgcc acttgtgaga ctgtgtccac gagatcggag aactcccctg ttgttatcaa
     1681 ttctccatta actcttatcc gttctcgcag tgattggaga tgtgggctgg tgtaaagacc
     1741 aaccttgtat ccggaggcct gcattatgtt tgaaatcata agggaagttg aacccttccc
     1801 ttttgtgcct gtcacatgaa tgtatggata gcaatactgg ggacttccga ccgattcaag
     1861 cagtggaatc atccttccaa gccccagcct ggaaccgagg tagtgaaggc ttccgagttt
     1921 gtcaagcgct tcacagtagt tcatttcagg ctccaagcga aagtgccagt tcattgaggg
     1981 cagatagttc ggcagaaagc tcttcctgcc ttgccctgtt cttttcaaca acctctggtt
     2041 tggctctctt tgcgaagtcg ggatttgaaa gaagagcctg gattttgtcc atttctgctc
     2101 tcgtcttttc gattgagcct gcaaccctct ttttctcctc gactacgtct attcttcccg
     2161 agagatcaag gaagaatttc atgtccttga acactgcaac aacagtgcct tccggctgtg
     2221 actgcacaag gttgaacccc tcaatcctgg cgagttttat cacaaagtct ttttctcttt
     2281 caagaactat tgcatcgttg ccttttgcca tgaatgagca tggaacaatt gcctggtgca
     2341 ccctgaggct tgccttgagg ttcctgattg attttacggc cccctgaagc acctccatct
     2401 cctccattgc ctgtttgtcg aaggtgagat cttcaggttt tggccaggcc gccacaatta
     2461 gggccttgcc ttcatgtggc agtttttgcc atatctcttc ggtcacgaat ggggctattg
     2521 gatggagtat tctgagagtg ttgtccagta cgagtttcaa gaccctctgt gtcctctcct
     2581 tgtccttccc gccagattga agcgggagtt tggacatttc cacataccag tcgcaaaaat
     2641 ctgtccagaa gaaatcatag atcgttctcg cagctttggc gaaatcgaag cagtcaaacg
     2701 aggctgtcac ttccctgaca gtttctgaca gttttgaaat gatccacctg tcggcaaatg
     2761 tcgggttttc gattggcccg ggcgtgtatc cttcaaggtt cattatggtg aacctcgagg
     2821 cattccagac cttgtttatg aagtttctgc cttcagtcag cgctttgtcg ccgaggctta
     2881 tgtcttgccc gccgagtgtc gagaggtatg tgagggtaaa cctgagcgca tctgcgccgt
     2941 atttgtcaat gacttcaatc gggtcgattg catttgcaag agacttgctc atctttcttc
     3001 cgtgttcgtc tcttatgagt cctgtcaggt acacatcatc aaatggtttc ttcccgacaa
     3061 gctccagccc ggagaagatc attctggcga cccacaggaa tatgatgtcg tatgcagtca
     3121 gaaggagtga gccagggtaa tagtattcaa aatcctcggt tttgtccggc c
//
LOCUS       DAOSRI010000206         3165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_27647_length_3165_cov_2.910289, whole genome shotgun sequence.
ACCESSION   DAOSRI010000206 DAOSRI010000000
VERSION     DAOSRI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27647_length_3165_cov_2.910289"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PK717_06450"
     CDS             complement(<1..780)
                     /locus_tag="PK717_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47333.1"
                     /translation="MKTTVGLLLAIGLALYPVAPSTFVQGSDNRRIIEDAPIITPQTV
                     ITYPDWVFPAASFTTYHYNDVYNLVQSDVVLKYTLDTASLSLPSSSWSYWQQIGIRVS
                     GAGSDFNPPIGSGCWMSYGAKDTVPTPGIQNSQDKFNLQYQGGRDERYYDSYSPITVN
                     SQVGTPTAWNNYGLWFDRDTVDPWQDDMWGCLDGWTYNTGGIYNVEIAYHAITPTQGT
                     AFATVNGVPVGFYTAWSANGPNHSYNIGMSFTGDMTQMQIFG"
     gene            complement(1069..2256)
                     /locus_tag="PK717_06455"
     CDS             complement(1069..2256)
                     /locus_tag="PK717_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47334.1"
                     /translation="MRIFKYIGGIFLSILIVLYFVAFLRFTSGFAPRRISPVSIDAKC
                     VIYDYAFTDSSIVDLRNQNVNIYRLYFEYFKSPDDKINSQISIPGKTISSYAISDISP
                     ESPEKPQTYHYVLYSQQNSKYYISLFNAPLLNAQIEKHWSVEVPKLKTIVTSTGKQAI
                     ICQDESMKDVIIYNIDGVCEYKKTFDGPVILTSIYTEDNKQKVLYSDGEHISSLDGEI
                     NIELPRKSNMEDSFFLPAQDPSNLDIFGYYDGEYLYGFDIKSAGTSLYSKKIAGLKPG
                     VYTNRYMCPYSHGIITINPRTGSLRPTYGYGVDLLGKTDFTFVSYFMRYEDTYCLFSG
                     SDGTNSSLSIYKINKNGMTSLMSSRYYQFGKIYYSFVEYCNFYVFTDKGVFSTDTFNY
                     FYD"
     gene            complement(2443..3078)
                     /locus_tag="PK717_06460"
     CDS             complement(2443..3078)
                     /locus_tag="PK717_06460"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR47335.1"
                     /translation="MEYILDDPKYLVLHHFVNGKIGVAKEKALVAATKKLNPREYHYV
                     VYSDGSWEGWQKEGLVAAHCGADNQSQSRCGAHNFNSIGVCCMGNFEKGPMEEKQLSG
                     LLALIKDIAARHGIVPENILPHSAIAPTACPGKYFPLLEARQTADIPLVRVAINSDQA
                     LVMGKPNKMPLKLALDMGHAVVPLRWFCEALGFRVVFLRDGGIVEVRKKFA"
BASE COUNT          873 a          735 c          650 g          907 t
ORIGIN      
        1 gccaaaaatc tgcatttggg tcatgtctcc ggtgaagctc atgccgatgt tgtaggaatg
       61 attggggcca tttgccgacc aggctgtata aaaaccaacc ggcacaccgt tgactgtcgc
      121 aaaagcagtg ccctgcgttg gggtgattgc atgatatgca atctcgacat tgtatatgcc
      181 gccagtattg tatgtccacc catcaaggca accccacatg tcatcttgcc agggatcgac
      241 tgtgtcacga tcaaaccaca atccataatt gttccatgcc gttggcgtgc ccacttgcga
      301 gtttactgtg atagggctgt acgaatcgta gtatctctcg tcacggccac cctggtattg
      361 gagattgaat ttgtcctggg agttttggat tcccggggta ggcacagtgt cttttgcgcc
      421 ataagacatc cagcaccccg aaccgatagg cggattaaaa tcagaccctg cccctgacac
      481 acgaatgccg atttgctgcc aatagctcca tgaacttgaa ggaagggaca gactggcagt
      541 atcaagcgta tatttcagaa caacgtcgct ctggaccagg ttatacacat cattataatg
      601 atacgttgta aacgatgcgg caggaaaaac ccagtcagga taagtgatga cagtctgtgg
      661 ggtgatgatg ggagcatctt ctataattct cctgttgtca ctcccctgca caaatgttga
      721 cggggcaaca ggataaagag ccaaaccaat tgccaaaaga aggcccactg ttgttttcat
      781 gagcataact ctccacctct ttttccagct tttttactca caataacagg tcatcattaa
      841 tttatgggca tgcctttgtc aatatgctaa aagtaattat tacttaataa ataactgtga
      901 tatctcataa atacaatatc tttgtctata tatttttata aataatagtt attagaaaca
      961 tatcctcgta aatattcatg gccagatgta tttttctata attaataatt gatatattat
     1021 cttttttgtt atttctatga ttattggttt gttaagaaat tttattatct aatcataaaa
     1081 gtaattgaac gtatctgttg agaaaacacc tttgtcggtg aacacataaa agttgcaata
     1141 ctcaacaaaa gaatagtata ttttgccaaa ttgatagtaa cgggaagaca tcaggcttgt
     1201 catcccgttt ttattgatct tgtaaatgga gaggctcgaa tttgtcccgt cactgcctga
     1261 aaacaggcaa taagtgtctt cataacgcat aaaatatgac acaaacgtga agtctgtttt
     1321 cccgagcaaa tcaaccccat atccgtaggt tggtctcaac gagccagtcc ttgggttaat
     1381 cgttattatt ccatggctat acggacacat gtacctgttc gtgtaaacac caggtttcag
     1441 accggcaatc tttttggagt ataatgaagt accagccgac ttgatgtcaa aaccgtataa
     1501 atactcgcca tcataatatc caaaaatatc tagattagac ggatcctggg ctggcagaaa
     1561 aaacgaatct tccatgttgc ttttcctcgg gagctctatg ttaatttctc catccaggct
     1621 ggatatgtgc tccccgtcag aataaagaac cttctgttta ttgtcctcag tgtaaatgct
     1681 tgtcagtatg accggaccat caaaagtttt tttgtattca cagaccccat caatgttgta
     1741 tataatgaca tccttcattg actcgtcctg gcaaatgatg gcttgctttc cagtggaggt
     1801 gacgatggtt ttgagttttg gcacctctac gctccagtgt ttctctatct gggcattaag
     1861 aagaggggca ttgaacagcg aaatataata tttgctgttt tgctgtgaat acaaaacata
     1921 atgatatgtc tgcggtttct ctggggattc tgggctgatg tccgatattg cgtagcttga
     1981 tatcgtcttt ccgggtatcg agatttgact attgatttta tcgtctggag atttgaaata
     2041 ttcgaaatag agcctgtaga tgttcacatt ttgatttctc aaatcaacta tgctgctgtc
     2101 tgtaaaagca tagtcataaa ttacgcattt tgcatcgatt gaaacagggg aaattctcct
     2161 tggcgcaaaa ccgctcgtga accgtaaaaa agctacaaaa tataatacga tgagtataga
     2221 caaaaatatg ccacctatgt atttgaagat cctcacaatt tatatattgg catcttttat
     2281 tttaaggttc aactggtggc tattctagtt cctcaaaatg gtattattca aaaaaaggcg
     2341 ttaggtaagt ttctcacaaa aagagaaaca caacaaaaaa actggcttgg gtatccccaa
     2401 gattacccag gccagccgat cactgaaatt acagctcaaa gcctacgcaa acttcttcct
     2461 cacctccaca atcccaccgt ccctgagaaa aacaaccctg aacccaagcg cctcgcaaaa
     2521 ccagcgcagg gggacaacgg catgccccat gtccagcgcc agcttcaacg gcattttatt
     2581 cggcttgccc atgacaagcg cctgatcaga gtttatcgcc accctcacaa gcgggatatc
     2641 cgccgtctgt cttgcctcta aaagtgggaa atattttccc gggcaagccg ttggtgcaat
     2701 cgcgctgtgt ggcaaaatat tctctggtac aatgccatgc cgtgcggcaa tgtcctttat
     2761 cagggcaagg agtcctgaga gctgtttttc ctccatcggg cctttttcaa agtttcccat
     2821 gcaacaaacc ccgattgagt tgaaattgtg agccccacac ctgctctgtg actgattgtc
     2881 agccccgcag tgcgccgcga ccaaaccctc tttttgccag ccctcccacg agccgtcaga
     2941 gtaaaccaca taatgatatt ccctggggtt gagctttttt gtcgccgcca caagggcctt
     3001 ttcctttgcc acaccaattt ttccgttcac aaaatggtgg agcacaagat atttcgggtc
     3061 atcaaggatg tattccactg ctcacctctc cggcgccgtc accagcgtca ccctcagcct
     3121 tggggtggac gctgttgccc agtacgccca gaccgccagc cctga
//
LOCUS       DAOSRI010000207         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_27797_length_3151_cov_4.329134, whole genome shotgun sequence.
ACCESSION   DAOSRI010000207 DAOSRI010000000
VERSION     DAOSRI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27797_length_3151_cov_4.329134"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK717_06465"
     CDS             complement(<1..584)
                     /locus_tag="PK717_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47336.1"
                     /translation="MMQSLTTRDRDKWLEVIKKVPSADCYAHPELGKMWEASYPYSFE
                     AVYAEFGDKVIFYPFLVRPLIELPFGKKIMEELGPCFDITSPEYGGIFHNFEDGVPYD
                     IVKQFNEEFGEFCKKRRIVTEFGRVQVLLSNTNLGPGYNVRRMGKVVWVDLTKTTEEL
                     EAGMTKEGRKKMRKAVRDGVSVREMGPEGFEQFPP"
     gene            complement(581..1108)
                     /locus_tag="PK717_06470"
     CDS             complement(581..1108)
                     /locus_tag="PK717_06470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOR47337.1"
                     /translation="MIVGAKTLVRAIRDDDLPKLVQWRNDKSIQQNLIGWHFPVSPED
                     EKLWLERVRSDRKNKRFAIEADSGEYIGNIGLYDIDFINRHCGFGIFIGDPRYRGGGY
                     ATDATIALVKFAIEELGMVRLWLTVLTTNDKAIKLYERVGFKLEGVLRRQNFRGGEYV
                     DENLMGLLKEELITT"
     gene            1680..2060
                     /locus_tag="PK717_06475"
     CDS             1680..2060
                     /locus_tag="PK717_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47338.1"
                     /translation="MIFGFDLVTTKRCWQTNEYGPILFVIPLQDGLAVFGHDGTSAVL
                     DSLTGEVRWRKETGAAATMPLFCFEKLFVATSNTLLCLDIADGHIIWAFETPDNIVAG
                     PSCRDGKIVIADSGGHVFCLAPPQ"
     gene            complement(2120..2890)
                     /locus_tag="PK717_06480"
     CDS             complement(2120..2890)
                     /locus_tag="PK717_06480"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOR47339.1"
                     /translation="MSRYTIGEFSRITGISAKMLRHYDGMGLLSPEFVGDNLYRYYTD
                     GQVLSGRTIAKLRDVDMPLVEIARLLEAMKRGQFPEDIVLEHMRRLGKRIDKDQKREK
                     LCRELIENKSLLEVIMNFESTIKEVKPMQVISVRKIGMYSEIGEMIQQAFFKAGCNCS
                     GAPFVLYHSEGAPEPADMEVCVPVSSGATITVPGVKALSVIYKGPYSGLGQAYKFGFD
                     QAQAKGLKISHPTREIYLTEPNDPQGPVTEILLPFEKM"
BASE COUNT          749 a          723 c          673 g         1006 t
ORIGIN      
        1 ggcgggaact gttcaaaccc ctcggggccc atttctctca cagaaacgcc gtccctgact
       61 gccttgcgca tcttcttgcg gccttccttg gtcattccag cctcaagctc ttccgttgtt
      121 tttgtgaggt cgacccagac gaccttgccc attcttctga cattgtagcc agggccaagg
      181 ttggtgtttg aaagaaggac ctggaccctt ccaaactctg ttacgatgcg cctcttcttg
      241 caaaactccc caaattcttc gttgaactgt tttacgatgt cgtagggaac accatcttca
      301 aagttgtgga aaatgccccc gtactctggt gaggttatgt caaaacatgg gccgagctct
      361 tccattattt ttttgccaaa tggcaattcg atgagcggcc tgaccaaaaa agggtagaaa
      421 atgaccttgt cgccaaattc tgcgtagact gcctcaaaag agtatgggta tgacgcttcc
      481 cacatttttc ccagctccgg atgggcatag cagtcagcgg aggggacctt ttttattacc
      541 tcaagccatt tgtccctgtc ccttgtggtc aggctttgca tcatgtggtg ataagctctt
      601 ctttcaaaag acccatcaga ttttcgtcca catactcccc acccctgaaa ttctggcgcc
      661 ttaggacacc ctcgagtttg aacccaaccc tttcgtagag ctttattgct ttatcgtttg
      721 tggtgaggac agttagccac agccttacca tcccaagctc ctcaattgca aatttgacca
      781 gtgcgattgt agcgtcggtg gcataaccac cacccctgta ccttggatcg ccaataaaaa
      841 tgccaaaacc gcagtgccgg tttatgaaat ctatatcgta aaggccaatg tttccgatgt
      901 actcaccaga atcagcctct attgcaaacc tcttgttttt tctgtcgctc cgcactcttt
      961 cgagccagag cttttcgtcc tcgggagaaa caggaaaatg ccatccaatc aggttctgct
     1021 ggatggactt gtcgttcctc cattgcacaa gctttggaag atcgtcgtcc ctgatggctc
     1081 tgaccagggt cttggcgcct acgatcaagg tcacctccaa tctaagataa tttatgttgc
     1141 ttgctcattt tggtcaagta ggttgcacct acttgcaaaa tattttaaca attttttcag
     1201 cgtgtcacaa tttgccagct tcaaatattt ttttaaatgg gatcttttgg ttttgtgata
     1261 gtggttgttg ttttttatag aaatattttt aattgtcgat tttcatcaaa ctggatattt
     1321 aaattttgta aacctgtatc ggaaatctgt ttccgcgtgc agcagaagta ttccagtttc
     1381 gaatgccagt ttggttatat caaaaaatag caatgaatcc tattttttgt tttaatattt
     1441 tatattatta ataattatta tcgaataata ttatatttgc ttgtctccta ctgttctatt
     1501 tttattaata ttattaaaat gtaagcgata tggaaaatgg taattgcaaa agtgtaaatc
     1561 gttcaagaaa agtcaagctt tttgacgatg gtttttcttt agactggcag atgaataccg
     1621 ggttgcccgc aaaacgaact tttctttttg agaaaacctg cactttattg agcaatgaga
     1681 tgatctttgg atttgatctt gtgaccacaa agagatgttg gcaaacaaat gaatatggcc
     1741 ccattttgtt tgtgattcct ctccaggatg gcctggcggt ttttgggcat gatggaacta
     1801 gtgctgtttt agacagcttg accggggaag tcaggtggcg caaggaaaca ggagcggctg
     1861 ccacaatgcc cctgttttgt tttgaaaaac tttttgttgc aacgagcaat actcttttgt
     1921 gtcttgatat tgctgatggc catatcatct gggcctttga aacaccagac aacattgttg
     1981 ccgggccctc ttgccgtgat ggcaaaatag ttattgccga ttctggcggg catgtatttt
     2041 gtcttgcccc gccccaataa atattgttag agttgtgatt gttttacagg cccccaaccg
     2101 ggggcctgtg ttttttgtgt cacattttct cgaatggaag caggatttcg gttaccggac
     2161 cttggggatc gttaggctct gtcagataga tttctcttgt gggatgcgat atctttaacc
     2221 cctttgcctg cgcctggtca aaaccaaatt tgtaagcctg gcctaatccg ctatagggcc
     2281 ccttgtaaat gaccgagagt gcttttacgc ctggtacggt tatggtggca cctgaagata
     2341 ctggaacaca tacttccatg tcggccggct ctggtgcacc ttcgctgtgg tacaaaacaa
     2401 acggggcacc gctacaattg cagccagcct tgaaaaacgc ttgctgaatc atctccccga
     2461 tctcggagta catcccgatt tttctgaccg aaatgacttg cattggtttt acttctttta
     2521 tcgtcgactc aaaattcatt attacctcca gaagtgattt gttttcgatg agctccctgc
     2581 acaatttctc tctcttctgg tccttatcaa tcctcttgcc gaggcgcctc atgtgctcaa
     2641 ggacgatgtc ttccgggaat tgtcctcttt tcattgcctc aaggaggcgc gcaatctcca
     2701 caagcggcat atcgacatct ctgagtttgg caatggttcg acctgataaa acttggccat
     2761 ctgtgtaata cctgtagaga ttgtcgccaa caaattcagg ggacaatagc cccatcccat
     2821 catagtggcg gagcatcttt gcgctgattc cagttattct tgaaaactca cctattgtgt
     2881 agcggctcat cgttgtcaca ttctggtcct tcccataatg ggaaggtcaa tagggttatt
     2941 ttaaatgttc gtggggccac cacaagatga acataaaatt tattgaatgg ggaatactta
     3001 ttttctattt tcgcttgaat gccttggatg tttgatagtg gatgtttcca aggatctgat
     3061 tatttctttt gagacctggt aatcaccttt tgtgatgtct tgcacctttt ttgcgatctg
     3121 gtttacccga tccggaacaa taccgtaata c
//
LOCUS       DAOSRI010000208         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_28075_length_3128_cov_3.460137, whole genome shotgun sequence.
ACCESSION   DAOSRI010000208 DAOSRI010000000
VERSION     DAOSRI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28075_length_3128_cov_3.460137"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK717_06485"
     CDS             <1..532
                     /locus_tag="PK717_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47340.1"
                     /translation="ELTQDEKRIADVIGKGPFLTVATKMDGQWKLMPFFGPELFEIKE
                     AEKAYIAYLDAIKSGQFSKTYDFTPTDLLSGASREQVEREWKNNNGKGIAEFLKKLDL
                     KPTAGRIEFGVNHNGRTYPYGVTFNLFMNIDQMSYQGNDTLIKMIADDWTTGKNTTVT
                     MIYEDGWKVVAENPLF"
     gene            complement(477..935)
                     /locus_tag="PK717_06490"
     CDS             complement(477..935)
                     /locus_tag="PK717_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR47341.1"
                     /translation="MRYELAATTFVFNGERFAIHFHEKVKKWLPPGGHVEQNELPHEA
                     AIREVIEELGVKPRLLFDLPPDYGIETVPLPVAILVENIDSEHCHTDMIYAAESDTKT
                     ILEPFSWVTIEEAESMGAPKDVVVLAKKCLEIVRKAGSRQQPSSHLHKSL"
     gene            complement(932..2161)
                     /locus_tag="PK717_06495"
     CDS             complement(932..2161)
                     /locus_tag="PK717_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR47342.1"
                     /translation="MSTFKLALRNLAKKPLRTVLAIATVTISITLLLSLLGFYFGYNK
                     AMKDELNNFGVQMLAVPKGCPYSSTTLLLHGGVIPNKLPLEAIDKIKAIQHVKSAQGI
                     VMGTLELDSVQHIIYGISPGFEKLRPNWKVQGKFPTAEGELVLGSTIAKKLGAEIGKK
                     YTVVGIAQDKKQQEELGTYPPIGQQVITYKTDPRELTVVGLLDQTGASEDNFFITNDD
                     EARKIIDLNGYTAIAIELDDQAGSKEFVAVAVEGINDAQPVTINQVANTVSGLIDSAR
                     SIMLSVVIVAIIACAIVVAGTALMGVIERSKEIGMMRTIGATPMQVGTSVGLEVVLLT
                     LIGGIIGIVLTLVLQNPLSDLLRILIPNAPNTNLVSLPPDLLLLSIGIAVALGVLASI
                     PPLISAVRAPPLRMAGS"
     gene            complement(2161..2565)
                     /locus_tag="PK717_06500"
     CDS             complement(2161..2565)
                     /locus_tag="PK717_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47343.1"
                     /translation="MKKISFVVALVVLFGAIAILASCGSSGPQEKTFGPPIEKPEEYQ
                     PATVEQVFADPELMDKQVVMDVYVDEVCPAGCWFFISDKLGDKVRLYVSRTKDSFTVP
                     ESIKGLHVKVYGKVTASNTGEMLEGHRVELID"
     gene            complement(2552..>3128)
                     /locus_tag="PK717_06505"
     CDS             complement(2552..>3128)
                     /locus_tag="PK717_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47344.1"
                     /translation="EMLSIMGRSGSGKTTLLSCIGMLENIDSGSLALEGRNIKDFSEA
                     QRTVLRRELMGFVFQRFFLIPTLTAYENVALPGLFAGKKLGKDHIMELLEMVGVSQRY
                     DHRPGQMSGGEMQRVAIARALALDPPILIADEPTGNLDTANAEAVFGLFKKLAGQGKT
                     IIIATHSQKLAKDCDRVVELMDGRITRDEKN"
BASE COUNT          772 a          754 c          745 g          857 t
ORIGIN      
        1 cgagctgacg caggacgaga aaaggattgc cgatgttatt ggcaaaggcc ctttcctgac
       61 ggttgccaca aaaatggatg gccagtggaa gctcatgcct ttctttgggc cggaactttt
      121 tgagatcaaa gaggctgaga aagcctacat tgcatacctt gatgccataa aaagcgggca
      181 gttttccaaa acctatgatt ttaccccaac tgaccttttg tctggtgcaa gcagggaaca
      241 ggtcgagcga gaatggaaaa acaacaatgg caagggtatt gccgagtttt taaagaagct
      301 tgacctcaag ccgactgctg gaaggattga attcggcgtc aatcataatg gaaggactta
      361 tccttacggg gttaccttca atctattcat gaacatcgac cagatgtcat accaggggaa
      421 tgataccctg ataaaaatga ttgccgatga ttggacaacc ggaaaaaaca ccacagtcac
      481 aatgatttat gaagatggct ggaaggttgt tgccgagaac ccgcttttct gacaatttcc
      541 agacactttt tcgccagcac cacaacatct tttggtgcac ccatcgattc agcctcctca
      601 attgtgaccc aggagaatgg ctccagaatg gtttttgtat ctgattctgc tgcgtatatc
      661 atgtcagtgt ggcaatgctc gctgtctata ttttcgacaa gaattgcgac aggaagcgga
      721 acagtctcta ttccgtaatc tggcggtaaa tcaaaaagga gcctgggttt gactccaagc
      781 tcctctatta cttcccttat cgccgcctca tgcggcagtt cattttgttc tacatgaccc
      841 cctggtggaa gccatttctt gaccttctcg tgaaaatgta ttgcaaacct ctctccgttg
      901 aaaacgaagg tggttgcagc cagttcatat ctcatgaacc tgccattcga agcggtggtg
      961 ccctgactgc ggatataagt ggtggaatag acgcaagaac acccagagca acggctatgc
     1021 caatcgaaag aaggagcagg tctggaggga gagataccag gtttgtgttt ggcgcatttg
     1081 gtatcaaaat cctgagcagg tcacttagcg ggttttgcaa gacaagcgtg agcacaatac
     1141 ctattatgcc acctatcaat gttagcaaga ccacctcgag accaaccgat gttcccacct
     1201 gcattggtgt tgccccgatg gttctcatca tgccgatctc ttttgatctt tcgatgacgc
     1261 ccatcagcgc agtgccggca acaacgatgg cgcaggcaat tattgcaact atgacaacag
     1321 aaagcatgat cgatctggcc gaatcaatga gaccagatac cgtgtttgca acctgattga
     1381 ttgtcactgg ctgtgcatca ttgatgcctt caacagcaac ggccacgaac tcttttgagc
     1441 ctgcctgatc atcaagttct atcgcaatgg cagtgtaccc gttcaggtca ataatttttc
     1501 ttgcctcgtc gtcgttggta atgaagaagt tgtcttcgga agcgccagtc tggtcaagaa
     1561 ggccaacaac ggtcagctcc cttgggtcag tcttgtaggt tatgacttgc tggccaatcg
     1621 gcggataggt gcccagttct tcctgctgtt ttttatcctg ggcaatccct accacagtgt
     1681 acttcttccc gatttctgcg ccgagttttt ttgcaatggt ggaaccgaga acgagttcac
     1741 cttccgcggt tgggaatttt ccttgtacct tccagtttgg tctgagtttc tcaaaacctg
     1801 gagaaatacc atagataatg tgctggacgc tatcaagttc gagggtcccc atcacaatac
     1861 cctgcgcact ttttacatgc tggattgctt ttatcttgtc aattgcctca agcgggagct
     1921 tgtttgggat gactccacca tgcaaaagga gtgttgttga ggaatagggg catccttttg
     1981 gcacggcaag catctgcact ccaaagttgt tcagctcatc cttcatggcc ttgttatagc
     2041 caaagtagaa acccaaaagg gacaagagga gcgttatgga gatagtgact gtggcaattg
     2101 ccagcactgt gcgcaagggt ttttttgcca gattgcgcaa ggcgagcttg aatgtcgaca
     2161 tcagtcgata agctccaccc tgtggccctc aagcatttct cctgtatttg aggccgttac
     2221 tttcccatag actttaacat ggagaccctt tatggattct ggcacagtaa aactatcttt
     2281 ggtcctggag acgtagagcc tgaccttgtc gcccagtttg tctgagatga agaaccagca
     2341 acctgcgggg cagacttcgt ccacgtagac atccataaca acctgtttgt ccatgagctc
     2401 tggatctgcg aatacctgct caacggttgc cggctggtat tcctcaggtt tctcaattgg
     2461 cgggccaaat gttttttcct ggggacccga tgacccacag gatgcgagta tggcaatagc
     2521 gccaaaaagt accacaagtg cgacaacaaa gctaattttt ttcatctctt gttattctcc
     2581 cgtccatgag ttctaccacc ctgtcacagt cttttgcaag tttctggctg tgggtggcga
     2641 taataattgt ttttccttgt ccagcaagtt ttttgaagag cccaaatacg gcttctgcgt
     2701 ttgccgtgtc aaggtttcct gttggttcat cagcgataag gattggtggg tcaagcgcca
     2761 gcgccctggc gatggcaacc ctttgcattt caccaccaga catctggcct ggtctatggt
     2821 catatctttg tgatactcca accatttcga ggagttccat gatatggtct tttccgagtt
     2881 ttttgcctgc gaaaagtccg ggaagtgcga cgttttcata tgctgtcaat gtgggaatca
     2941 ggaagaatct ctggaaaaca aaacccatga gttccctgcg caaaactgtt ctttgggcct
     3001 cagagaagtc cttaatgttc cttccttcaa gggccaacga tccagaatca atgttctcga
     3061 gcattccgat gcaggagagc agggttgttt ttccagaacc cgatctaccc attattgaga
     3121 gcatctcc
//
LOCUS       DAOSRI010000209         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_28413_length_3099_cov_3.722733, whole genome shotgun sequence.
ACCESSION   DAOSRI010000209 DAOSRI010000000
VERSION     DAOSRI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28413_length_3099_cov_3.722733"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK717_06510"
     CDS             complement(<1..289)
                     /locus_tag="PK717_06510"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOR47345.1"
                     /translation="MHRFYVQGGKPVLKEGDFQHAIVVLRLEEGKEIEIIQGFGQRHL
                     AKIGKIDLKAKQAELIIGEKIEESVEPPCKITLIQGIPRMDKLGDIVESCTQ"
     gene            complement(289..1308)
                     /gene="hrcA"
                     /locus_tag="PK717_06515"
     CDS             complement(289..1308)
                     /gene="hrcA"
                     /locus_tag="PK717_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOR47346.1"
                     /translation="MRENRSRSELILTILVSLYIEKGEPISSEEITSKMPFKVSPATV
                     RNELAALEEQGLLLRQHVSSGRIPSYRAYRLYVDRLLDSGKLKPASLNVELPEHRRLE
                     AVLRAVSVTLSEHTKTASLVMFPLARKLKIKSFHLLGISPNRVLLVIVVNAEDIREFI
                     LSMPIVPDQDLLDRISRAIYSKIEPRQNWAPIQVLQDIVRDNQDLAEEKDLILNVLQL
                     IREIASKEAVQIYMEGTHRLLLNPELADLNKARHLIAALSAQDGLRDLLLDTVTKGKL
                     DIVLGSEAGRPEMEDVAYIGAPYQVDDAIGVLSLIGPLRMNYVLMFGALTTIASKLEK
                     ELKKE"
     gene            1431..1883
                     /locus_tag="PK717_06520"
     CDS             1431..1883
                     /locus_tag="PK717_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOR47347.1"
                     /translation="MNKDNTKKIARRTTVQRMAVLEELKKMLTHPTADELYTKVKHKL
                     SGISLSTVYRNLEILCEEGLIKRLQFSSGQTRYDATMSTHHHIRCVKCGRVGDIDFEQ
                     TQRIIELANKKSGYAVLDCKLEFIGICPKCQKNEKPDEKEIDTEKIKI"
     gene            1928..2503
                     /locus_tag="PK717_06525"
     CDS             1928..2503
                     /locus_tag="PK717_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR47348.1"
                     /translation="MSNLKGSMTEKNLLKAFAGEAQARNRYTYFASKARDEGFMQISD
                     IFEETANQEKEHAKRIFKFLEGGDLEVTATFPSGIISTTLENLLAAASGENHEHSVMY
                     PEFAKVAEKEGFVQIAAVFTAIANAEKQHEKRYRALAANIEKGKVFSKEHKAVWRCRN
                     CGYLHEGTEAPEICPACNHPMAYYEILAENW"
     gene            2537..2917
                     /locus_tag="PK717_06530"
     CDS             2537..2917
                     /locus_tag="PK717_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOR47349.1"
                     /translation="MANKLEIYKCEICGNIVEVIHGGVGTLVCCNKPMVLQQENTVDA
                     SKEKHVPVIEHTPDGVKVKVGAVAHPMEEKHYIEWIQIIFGDKACRQFLKPGDPPEAT
                     FNVHPDKVVAREYCNIHGLWKGEM"
     gene            2918..>3099
                     /locus_tag="PK717_06535"
     CDS             2918..>3099
                     /locus_tag="PK717_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOR47350.1"
                     /translation="MVPNKMHELMNDQIKHELESYYLYLNMAAYFHDQTLDGMASWMR
                     AQAHEEMIHAMKFFNHL"
BASE COUNT          952 a          663 c          698 g          786 t
ORIGIN      
        1 gctgtgtgca tgattcgaca atgtctccga gcttgtccat tctcggtatt ccctgaatca
       61 gtgttatttt gcagggcggt tctacgctct cctctatctt ttcaccaata atcagctctg
      121 cctgtttggc tttaaggtca atcttgccta tcttggccaa gtggcgttgt ccaaaaccct
      181 gaataatctc tatctccttg ccctcttcta gcctgaggac aacgattgca tgctggaaat
      241 ccccctcttt caggacaggc ttgccgccct gtacatagaa cctgtgcatt attctttctt
      301 gagctccttt tcgagtttac ttgcgattgt tgtgagtgct ccaaacatga gtacgtaatt
      361 catcctgaga ggtcctataa gcgaaaggac gcctattgca tcatccactt gatatggggc
      421 accaatatac gccacgtcct ccatttcagg acgtcctgct tcagaaccaa gaacaatgtc
      481 caattttccc tttgtaacgg tgtcaagaag aagatctctc aatccatcct gtgcagaaag
      541 agcagcaata aggtgccttg ccttattgag atcggcaagc tcaggattca gaagcaatct
      601 atgggtgcct tccatatata tctggacagc ctcttttgag gctatctctc ttatcaattg
      661 tagaacattg agaatcaggt ccttttcttc tgcaaggtct tggttgtcac gaacgatatc
      721 ctgcaaaacc tggattggtg cccaattctg tctgggttct atttttgaat atatggccct
      781 tgaaatcctg tccaggaggt cctgatcagg cacaattggc attgaaagaa taaattccct
      841 tatatcttca gcattgacaa caataaccaa gaggaccctg tttggcgaaa tacccaaaag
      901 atggaaggat tttatcttga gctttctggc aagtggaaac atgacaagcg atgcagtttt
      961 tgtgtgttca gaaagcgtca cagaaacagc cctgagtaca gcttcaagcc tgcggtgttc
     1021 cggcaactca acattcaaac tggctggttt caatttgcca gagtcaagaa gcctgtctac
     1081 ataaagcctg tatgctctat aactcggtat cctgccagat gaaacgtgct gtctcaaaag
     1141 cagaccctgt tcctcaagtg ccgccagttc attgcgaacc gtagcaggtg agaccttgaa
     1201 tggcatcttg gaggtgatct cttctgatga tatgggctcg cctttttcaa tataaagact
     1261 gactaaaatg gtcaatatca gctctgacct tgacctgttt tctctcattt cttctatgat
     1321 attagcaaag attcctgcaa atctgtgtta agaatgtgaa atgattactt gttaactttt
     1381 taagattgac aaaaataata gagttgctat tattaataat agtttgcgtt atgaataagg
     1441 ataacacaaa aaagattgct agacgaacaa ctgtacagcg gatggcagtc cttgaggagc
     1501 tgaaaaagat gctcacccat ccgacagctg atgaattgta cacaaaggtg aaacacaaac
     1561 tctccgggat aagcctgagt acagtatata ggaaccttga aatactttgt gaggaagggc
     1621 tcataaaaag gctgcaattc tcatcaggtc aaacgcgtta tgacgcaacc atgtcaactc
     1681 accatcatat aaggtgcgtg aaatgtggaa gggtaggcga catcgatttt gaacaaaccc
     1741 agcgtatcat cgagcttgca aacaagaaaa gtggctacgc ggtacttgat tgcaagctcg
     1801 agtttatcgg aatctgccca aaatgccaaa aaaatgagaa acccgatgaa aaagagattg
     1861 atactgaaaa aattaaaatt tgaaaaaccc aaaaacattg tctaaaatat aaaacaagga
     1921 ggaaattatg agcaatttga aggggtcgat gactgaaaag aatctgttaa aagcttttgc
     1981 tggtgaggca caggcaagaa accgctacac ctattttgcc tcaaaggcaa gagatgaagg
     2041 gttcatgcaa atctctgaca tctttgaaga aacagcaaat caggagaaag agcatgcaaa
     2101 aagaattttt aagttcctcg agggcggcga tcttgaggta acagccactt ttccttcagg
     2161 aatcataagt acaacgcttg agaacctact tgcagcggcg agcggcgaga accatgaaca
     2221 ttcagtaatg tatccagagt ttgccaaagt ggccgaaaaa gaaggatttg ttcagatcgc
     2281 tgcagttttt acggcgattg ccaacgccga aaaacaacac gagaaaagat accgcgcatt
     2341 ggcggcaaac attgaaaagg gaaaggtttt tagtaaagag cataaggcgg tctggagatg
     2401 caggaactgc ggctacctcc acgaagggac agaagcacca gaaatttgcc cagcctgtaa
     2461 ccatccaatg gcttactacg aaattctggc agaaaactgg taataaacaa atctagaaaa
     2521 ggaaagagga gggttaatgg caaacaagct tgagatttac aagtgcgaaa tttgtggaaa
     2581 catcgttgag gtcattcatg gcggcgttgg aacactcgtc tgctgcaaca aaccgatggt
     2641 cctccagcag gaaaacacgg ttgatgcttc aaaagaaaag catgttccag tcattgagca
     2701 tacacctgac ggggtaaagg taaaagttgg ggccgttgcc catccgatgg aagaaaaaca
     2761 ctatattgaa tggatacaga taatatttgg tgacaaggca tgcaggcaat tcctgaaacc
     2821 gggtgacccg ccagaggcga cattcaatgt ccatccggac aaggtggtgg caagggaata
     2881 ttgcaacatt cacggcctct ggaaaggaga gatgtaagtg gtaccaaaca aaatgcacga
     2941 gctgatgaac gatcagatca aacatgagct tgaatcctat tacctctacc tgaacatggc
     3001 ggcatatttc catgaccaga ccctcgacgg gatggcaagc tggatgaggg cacaggcgca
     3061 cgaagaaatg atccatgcaa tgaaattttt caaccacct
//
LOCUS       DAOSRI010000210         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_29444_length_3021_cov_3.579906, whole genome shotgun sequence.
ACCESSION   DAOSRI010000210 DAOSRI010000000
VERSION     DAOSRI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29444_length_3021_cov_3.579906"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..131
                     /locus_tag="PK717_06540"
     CDS             45..131
                     /locus_tag="PK717_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47351.1"
                     /translation="MLKEQLKWNYIVGFVFMIAAVFFVCKKW"
     gene            174..2363
                     /locus_tag="PK717_06545"
     CDS             174..2363
                     /locus_tag="PK717_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47352.1"
                     /translation="MRLFLALSVFLTLFPANISHGQTVSDFTPPVWLKTMPIQLEGSV
                     VSSPVIADGKIVIGTTAGKLYCIGLSPFEIVWTRDNEGSFRSSCLIANNKIYTGDSLG
                     RVYCRNLDDGEIIWGPQEFGEWTYSTPILNDGKIIFGANDRKIHALDAKTGEKIWETE
                     ELRGQIQAGVALCNEGYIIGCLGQPDSESDYDPGNLYCITADGKILWGPKVTKGPVQG
                     TPSVFGEHAFAASYGYSWENDRRPKGRVTCFRTSDGASIWQKDLEKEIWGSPATNGDC
                     VITGSRDHFIWCLDANSGEERWHFQTGGMVDATAVIENGTCWIGGMDGNLYALDLQSG
                     KKLQEINVASMGLGPQNSVAISEGHLVCASLSGTLYRFDLAPSCLVRPTKSSYKSYDG
                     SYHFEIRVENARKDGSPNLEATLTPINCFFKEDPQKVLVPPGKYETITFEGKIDPEPG
                     VYSCEIEVSSNSPTIILQDENGNTTNKLVLELNIKTHNPRLGKLPERIDFGAFWDDET
                     PKQKMILIQNEGDGAIQASVSPEKGLLPDKSTFTITNDTPVALGVALDVNSLPYGTFE
                     RTLDVKTNAGNSKIVFAGEKLRSKNVVEVWIGKKTVRINGQEKTLTQAPYIKNSLTMV
                     PLRLVSEGLGLEVSWVPEVKTIIINLFSGWYLRLIIGYKKALIERPDGGIDELQVQAP
                     PEIISGTTFVPLRLVGDALSRGGAKIDIAWNSQIRKATITRSLKTGK"
     gene            2400..2627
                     /locus_tag="PK717_06550"
     CDS             2400..2627
                     /locus_tag="PK717_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47353.1"
                     /translation="MAKTKEEKMGKIAGMRIIAIILFVMLATQVTTGLAGIAFDLHYW
                     LGIAIGVAVITHLAVNWGWIKTNIFGIKPKA"
     gene            2632..>3021
                     /locus_tag="PK717_06555"
     CDS             2632..>3021
                     /locus_tag="PK717_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR47354.1"
                     /translation="MEFFLPNLETDRLVLRMLSVNDAFDMYEYARDPEICKYVTWNAH
                     ESVEDSMSYLSLVEERYKGGEYFDWGIVLKKIDKLIGTCGFVDINKASSRGEIGYVLS
                     KTHWGKGLMSEAVRKVCSHGFESMELNR"
BASE COUNT          923 a          628 c          755 g          715 t
ORIGIN      
        1 acctggtcct gagcgtggga ggtgatggca cttttcttga caccatgctg aaagaacaac
       61 tgaaatggaa ttacatcgtt gggtttgtat tcatgattgc tgcagtattc tttgtttgca
      121 agaaatggta acggggaatt gacaaaacag gccaaagatg gtttgatttg tgcatgagat
      181 tgtttttggc tttatcagta tttcttactc tttttccagc aaacatctct catggacaaa
      241 ctgtttccga ttttacacct ccagtctggc tcaaaaccat gccaatccag ctggaaggga
      301 gtgtggtctc gtccccggtc atagctgatg ggaaaattgt aatcggaaca acagctggaa
      361 aactctactg cattggactt tcgccattcg agattgtctg gacaagagac aacgaaggct
      421 cttttcgttc ttcctgcctc attgcaaaca acaagatcta taccggcgac tcgcttggaa
      481 gggtttattg cagaaacctg gatgatgggg aaattatctg gggcccccag gagtttgggg
      541 agtggacata ctccacgcca attctaaatg atgggaaaat aatttttggg gcaaatgaca
      601 gaaaaatcca cgctctggac gccaagacag gcgaaaaaat atgggaaaca gaagagttga
      661 gaggacaaat ccaggcaggg gtcgcacttt gcaatgaagg ctacatcatt gggtgccttg
      721 gccagcctga cagcgaatct gattacgacc cgggaaatct ctattgcata accgctgatg
      781 ggaaaatatt gtgggggcca aaggtcacca agggacctgt gcagggcaca ccctctgttt
      841 ttggcgaaca tgctttcgct gcatcatacg gctactcgtg ggaaaacgac aggagaccta
      901 agggaagggt gacctgtttc aggacatctg acggggcgtc aatatggcaa aaggaccttg
      961 aaaaggagat atggggaagt ccggcaacaa atggcgactg cgtcataaca ggctcaagag
     1021 accactttat atggtgtctt gatgcaaata gtggtgagga aagatggcac ttccagaccg
     1081 gtgggatggt cgatgccaca gcagtcatcg aaaacggcac ctgctggatc ggtgggatgg
     1141 atggcaacct gtacgccctg gatttacaat ccggtaaaaa actacaggaa ataaacgttg
     1201 catcaatggg ccttggacca caaaactcgg tcgccatctc tgaaggacac ctcgtttgcg
     1261 caagcctgtc aggcaccctg tacaggtttg acctagcccc ttcatgcctt gtcagaccaa
     1321 caaaatccag ctacaagtcg tatgatgggt catatcattt tgaaataagg gtcgagaacg
     1381 caagaaaaga tggctctccc aacctcgaag caacacttac accaataaat tgctttttta
     1441 aggaagaccc gcaaaaagtt cttgtgccac cagggaaata cgaaaccata acatttgagg
     1501 gcaagattga cccggaacca ggcgtttaca gttgtgaaat tgaagtgtct tccaacagcc
     1561 caacaatcat actgcaggat gagaatggca ataccacaaa caaacttgtg cttgagctca
     1621 acataaaaac acacaaccca aggcttggta aacttcctga gagaattgat tttggggcat
     1681 tctgggatga cgaaacacca aaacagaaaa tgattttgat tcaaaacgaa ggcgatgggg
     1741 ctatccaggc cagcgtttca ccggaaaaag gccttttgcc agacaaaagt actttcacaa
     1801 taacaaatga cacgccagtg gcccttggcg tcgcgcttga tgtcaattct ttgccctatg
     1861 gcacttttga gaggacactc gatgtcaaaa caaacgctgg caattcaaaa atcgtctttg
     1921 ctggcgagaa gttgaggtca aaaaatgttg ttgaggtctg gataggcaag aaaacggtca
     1981 ggataaatgg gcaagaaaag acactcactc aggcaccata cataaagaat agcctgacca
     2041 tggttccgct caggcttgtt tcagaaggac tggggcttga ggtaagctgg gtgccggaag
     2101 tcaaaacaat catcatcaac ctgttttcgg gctggtatct cagactgata attggctaca
     2161 agaaagcact aatagagcgc cctgatggcg gaatcgacga actgcaggtg caggctccac
     2221 cagaaataat ctctgggaca acatttgtgc cattgagact ggttggcgat gccctatcaa
     2281 gaggcggagc gaaaatagat attgcgtgga acagccagat cagaaaagcc accatcacaa
     2341 gaagcttgaa aacaggaaaa taggaaggtg tttgaccgga agccaccata aaaattacaa
     2401 tggcaaaaac caaggaggaa aaaatgggca agatagcagg aatgaggatt atcgcaataa
     2461 tactttttgt gatgctggca acgcaagtta caactggcct agctggaatt gcatttgacc
     2521 ttcattattg gctcgggatc gcaataggtg ttgcggtcat tacacatctt gcagttaatt
     2581 ggggctggat aaaaacaaac atttttggaa taaaaccaaa agcatagttt catggaattt
     2641 ttcctcccaa atctcgaaac tgacaggctt gtcctcagga tgctgtctgt caatgacgct
     2701 tttgacatgt atgagtatgc cagggaccct gaaatctgca aatacgttac ttggaacgcc
     2761 cacgaatcgg tagaagattc catgtcatac ctgagtcttg tcgaggaaag gtataagggg
     2821 ggagaatact ttgattgggg tattgtactc aagaaaattg acaagctgat tggaacatgt
     2881 ggttttgtag acataaataa ggccagttca aggggtgaaa ttggctatgt tctctccaaa
     2941 acacattggg gaaaagggct aatgtccgaa gcagtaagaa aagtatgttc ccatggtttt
     3001 gaatccatgg agttaaacag g
//
LOCUS       DAOSRI010000211         3018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_29476_length_3018_cov_3.427945, whole genome shotgun sequence.
ACCESSION   DAOSRI010000211 DAOSRI010000000
VERSION     DAOSRI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29476_length_3018_cov_3.427945"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1859
                     /locus_tag="PK717_06560"
     CDS             213..1859
                     /locus_tag="PK717_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOR47355.1"
                     /translation="MENMVTCRPSIPFTGYRRIKGRPDSAALRVLVMTQADGHYADDG
                     SICYNFTKQRKIERCKTLLRRAGLVYTLSVYDQADRKYHRFRISNMDVPLWLRQFRSK
                     TYGSWLFDESSDAFFDELPHWDGYRPAPNSIQYCTCSKENADMVQAFAHLSGRAAGIR
                     LKKLSENHENWQDAHVVDIWLTPGNCHEIRNKPVVKPFNGKVYCAETLTGFFLVRRDG
                     RVWVTGNSGRLIQMQNLPQNHLQDLGTARALVGSGDYEAVKMFYGDVPDTLSQLVRTA
                     FIPRKGYRFIVSDFSAIEARVLSWLAGATWRMEVFANNGDIYCASASQMFKVPVEKHG
                     RNAHLRQKGKIAELALGYGGAVGALKAMGALEMGLKEEELKPLVDVWRQSNPNIVKFW
                     WDVDEAAKQAVRNKTATSTHGIRFSYESGFLFIILPSGRRLAYVKPCIGTNDFGSDCV
                     TYEGVGATKKWERIDTFGGKLVENVTQAISRDLLCHAMQQLEAAECRIVMHIHDEVVI
                     EAPKAMEVDEVGRIMGIVPSWADGLMLSAAGYEAEFYMKD"
     gene            complement(1955..>3018)
                     /locus_tag="PK717_06565"
     CDS             complement(1955..>3018)
                     /locus_tag="PK717_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47356.1"
                     /translation="SEFSSGKGWEPIGETDGDGYADSEYFSGSFNGNGYTISNLYVNR
                     PEKGRTGLFGYVNLARGNSINDVNLQDAVVIGSVSAGSLIGEIVSGNAVECTVNYSNI
                     RGTENIGGLIGKASNSTILHGYARNVYIGLNPSYNRAVNVGGLVGTSLEANITYCLTE
                     GMIVGLQKVGGLIGYVRGGSILKSVSWVSYVEAQDTVGGLIGDVYTGSIEECSAHSSV
                     VGDSIVGGLVGKTSFRYTSIRFCSATGSVTGGGLHGGLVGLHGGGVITTSFSRGTVTT
                     TPDGWGGGGLVGGIWNMPSIDNSYYDSTTSGRTDVGKGFPRTADQMRMQSTFIGWDFT
                     NIWVMGPSFPILRNILSLY"
BASE COUNT          822 a          712 c          725 g          759 t
ORIGIN      
        1 gctgggagcg tctggatgcg tggaggggtg gcctgatcgc aagttggagc cctaccaacg
       61 agggtgtggt gttttcccat gcaaaggctc tctgcttcga ttacgagggg cccatgtacg
      121 aataccgtag caatcgcata gcccagatca gcacacccga tcacaagatg tattttaaaa
      181 gacgctatgg caatccatgg attacggaca ctgtggaaaa catggttaca tgcaggccaa
      241 gcatcccttt tactggatac aggagaatca aggggagacc tgacagcgct gcactgaggg
      301 tgcttgtcat gacgcaggca gacggccatt acgccgacga tggctcgatc tgctacaact
      361 tcaccaagca gaggaagatt gaacggtgca agacactgct taggagagct ggtctggtat
      421 ataccctttc cgtctatgat caggcagaca ggaaatacca ccgcttccgt atttcaaaca
      481 tggatgtccc cctgtggctt cgacaattcc gtagcaagac ttacggctct tggttgttcg
      541 atgaaagctc cgatgccttt ttcgatgaac tgcctcattg ggatggctat cgtccggcac
      601 caaacagcat tcaatactgt acctgcagca aagagaatgc tgacatggtt caggcgtttg
      661 cccatttgtc agggagagct gcaggcatca gactgaaaaa attgtcagaa aatcatgaaa
      721 actggcagga tgctcatgta gtggatatct ggctgactcc aggaaactgc catgaaatca
      781 gaaacaaacc agtagtaaag cctttcaatg gaaaagtgta ttgtgcagag acgctgacag
      841 ggtttttcct tgtaagaagg gacggccggg tatgggtgac gggcaattcc ggcaggctga
      901 tacaaatgca aaatctgcca cagaaccatc ttcaggacct tggcacagcc agggcgttgg
      961 tgggaagtgg tgattacgag gctgtgaaga tgttctatgg tgatgtccct gatacgcttt
     1021 cccagctcgt gcgcacagcc ttcataccca ggaaaggata taggttcatc gtatcggact
     1081 tctcagccat tgaggcacgg gtgctctcat ggcttgccgg ggcgacctgg cgcatggagg
     1141 tctttgcaaa caacggtgac atctactgtg cgtcagcatc acaaatgttc aaggtcccag
     1201 tagagaaaca tggtcggaat gcccatctga gacagaaggg gaagattgcc gaattggcgc
     1261 tcggttacgg cggggcggtc ggagcactga aagcaatggg agccctggaa atgggtttga
     1321 aggaagaaga gctcaaaccc ttggttgatg tctggcgcca atccaatccg aacatcgtta
     1381 agttctggtg ggacgtggat gaggcggcaa agcaggcggt acggaacaag accgcaacca
     1441 gtacgcatgg gatccggttc tcctacgaga gcggttttct gttcatcata ctgccgtcgg
     1501 gcaggaggct tgcctacgtg aagccttgta tcggaaccaa tgactttggt agtgattgtg
     1561 ttacctatga gggagtgggc gctacgaaga aatgggaacg gattgatact tttggtggaa
     1621 aattggttga gaatgttacc caggcgatca gccgcgacct gttgtgccat gccatgcagc
     1681 agcttgaagc tgccgagtgc cgcatcgtga tgcatatcca tgacgaagtg gtcattgagg
     1741 caccgaaagc aatggaggtc gacgaagtcg gaaggatcat gggcatcgtg ccatcgtggg
     1801 ctgatggctt gatgctgagt gcggcgggct atgaggcgga gttttatatg aaagattgaa
     1861 atacgaatct caatcgatgg ctttttatag caatggttag tagtcatcat ttttaagtaa
     1921 aaaacatatc tttcgatttt ggtttcctcg aattttaata aagcgagaga atgttgcgta
     1981 atattggaaa gctcgggccc ataacccaga tattggtgaa atcccatcca ataaaggttg
     2041 attgcattct catttgatca gcagttcgag ggaagccttt acccacatct gtccttcccg
     2101 aggttgtact gtcataatat gagttatcaa tactcggcat attccaaatg cccccaacaa
     2161 gtccgccgcc accccaaccg tccggggttg tggtaacagt accacgtgaa aaactcgttg
     2221 tgataacccc tccaccgtgt agaccaacta aacctccatg aagtcctcca cctgttacac
     2281 ttccagttgc ggaacaaaat ctaatagagg tatatcggaa agaagttttc cccacaagac
     2341 cgccaacaat tgaatctcct acaacacttg aatgagctga acattcttcg attgagcctg
     2401 tgtagacatc accaattaaa cctcctacag tatcttgagc ttcgacataa ctaacccatg
     2461 atactgattt taatattgaa cctcctctaa catatccaat taatcctcct actttttgta
     2521 atccaactat catcccttct gtcaaacagt aagtaatatt ggcttctaaa ctagttccaa
     2581 ctaaccctcc aacattgaca gctctattgt atgaaggatt taagcctata taaacatttc
     2641 tggcatatcc atgaagtatc gtactatttg aagctttccc gattagtccc ccaatattct
     2701 ctgtgccacg aatgtttgaa taatttacag tgcattcaac agcattgcca ctaacgattt
     2761 ccccgattag actgcctgcc gaaacactcc ctatcacaac tgcatcctgc aaattcacgt
     2821 catttattga attgcctctt gctaaattca catatccaaa caaccccgta cgcccctttt
     2881 caggtctgtt aacataaagg ttggatatgg tatacccatt tccattaaat gagcccgaga
     2941 aatattcaga atcagcatat ccgtccccat cagtctcacc aataggttcc caaccttttc
     3001 cagatgaaaa ttcagaga
//
LOCUS       DAOSRI010000212         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_29974_length_2980_cov_3.832821, whole genome shotgun sequence.
ACCESSION   DAOSRI010000212 DAOSRI010000000
VERSION     DAOSRI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29974_length_2980_cov_3.832821"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK717_06570"
     CDS             <1..343
                     /locus_tag="PK717_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOR47357.1"
                     /translation="VNPLDRRYHAQTIACPDCGPTVFMGELRGQEAILGAKKSLLAGE
                     IVAIKGIGGFHLACIATNEASVNALRMLKNRGNEPMAVMCFDIIQVRGQAEIGLCEEQ
                     SCSPWRKPAVL"
     gene            340..1692
                     /locus_tag="PK717_06575"
     CDS             340..1692
                     /locus_tag="PK717_06575"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOR47358.1"
                     /translation="MKKSNNYSLAPSVAPGTDKIGIFLPYTPLHVLLLDGLPPVVLTS
                     ANLHEEPIIADGAKNIMVTKVLDNDREIVVPIDDSVTRCFKGKQQLFRRARGFVPETL
                     PIPKGPSILALGGQMKNTFTFAYDGKAMVSHHIGEMGNAATFARFAESLCLFRELFGF
                     KEEYCAHDAHPSYTTTVHRKELSRSDRFIPVFHHHAHIASVMGEHGLDGRVIGFGADG
                     TIWGFEFLVASRSEFERIGHLRTFPLPGGEIAIKDCRRILFSLARQAGSKIDLDIGPQ
                     ERELWGAMIDKGINAPATSSLGRLFDGFAVLAGLGQKATYEAELAVALESAYDPGARP
                     FNFPVRDFGDHFEVDWTVALKQGIENPASISGGFHKGISEAIVKGCLFARQKTGLKRI
                     CLSGGCFLNLILLELVFDALSSEGFEVYTNISLPPGDGCVSFGQAVVALSRVGGMRCV
                     "
     gene            1683..1907
                     /locus_tag="PK717_06580"
     CDS             1683..1907
                     /locus_tag="PK717_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOR47359.1"
                     /translation="MCLAVPAKICEKNGLDATVEFGGIKRQVRLDLLPDAEVGEFVLL
                     HTGFAIEKVNKEEAENILKTIQEVFGDVPV"
     gene            1891..2967
                     /gene="hypD"
                     /locus_tag="PK717_06585"
     CDS             1891..2967
                     /gene="hypD"
                     /locus_tag="PK717_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOR47360.1"
                     /translation="MTSQSDVALKLRGMIEELAVSPMTLMEVCGTHTHAIFANGIRAL
                     LPKTIKIISGPGCPVCVTSQSDVDRMIWLASQKNVVICTFGDMIKVPGKGGSLAVARS
                     MGSNIRVMYSPFDVIEWARQEKGKEFVLVGVGFETTTPGFAATIKQSKALGIKNLSIL
                     SLNKTVPEALRALAQSGEVKLDGLILPGHVSAIIGLGPYGFLAKEFGVPGVVTGFEGF
                     DILYGLAVLVKMIAEKKPAIVNTYTRSVKPDGNPTAMSLMGEVFDKSPAVWRGIGTIE
                     GSGLALSGEYAGFDAAKRFEMPNFPDEEPKGCLCGKVITGAVTPEECPLFGVSCTPSD
                     PVGPCMVSGEGTCSAYFKFRKGLR"
BASE COUNT          741 a          632 c          820 g          787 t
ORIGIN      
        1 tgtgaaccca ttggacaggc gctaccatgc ccagaccatc gcttgccctg attgtggtcc
       61 gactgttttt atgggagagc tgagggggca ggaggcaatc ctgggagcaa aaaagagtct
      121 tcttgctggt gaaattgttg cgatcaaggg aattggtggc tttcatctgg catgcattgc
      181 caccaatgaa gcctcggtca acgctttaag aatgctcaag aacagaggca atgagccgat
      241 ggccgtcatg tgttttgata ttattcaggt gagagggcag gcagaaattg gtctctgtga
      301 agaacagtcg tgctctccct ggagaaaacc tgctgtactt tgaaaaaatc aaacaactac
      361 agtcttgccc catcggttgc accaggcacc gacaagatcg gtatcttctt gccttataca
      421 ccgctccatg ttctcctgct tgatggcctt ccaccagttg tcctcacctc ggcgaacctc
      481 catgaagagc cgataattgc tgatggcgca aaaaacatta tggtaacgaa ggttttggac
      541 aatgaccgtg aaattgttgt tcccattgat gattcggtta caaggtgttt caagggcaag
      601 caacagcttt tcagaagggc cagaggattc gttccggaga cacttccaat tccaaaagga
      661 ccaagcatac ttgctcttgg tggccagatg aagaacacat ttacttttgc ctatgatgga
      721 aaggcaatgg tttcgcacca catcggtgag atgggcaacg cggcaacgtt tgcaagattt
      781 gccgagagcc tttgcctttt cagagagttg tttggtttca aggaagaata ttgtgcccac
      841 gatgcgcacc cttcctacac aacaacggtc caccgcaagg agctttccag atctgacagg
      901 tttattccgg tatttcacca tcatgcccat attgcatcgg tgatgggcga gcacggactt
      961 gatggcaggg tgataggttt tggggcagac gggacgatat gggggtttga gtttcttgtt
     1021 gcttcaaggt cagaatttga gagaattggt catctaagga ctttcccgtt gccaggtggc
     1081 gagattgcga taaaagactg cagaagaata cttttctccc ttgctaggca agcagggtcc
     1141 aagattgatt tggacattgg cccgcaagaa agggaacttt ggggtgcaat gattgacaag
     1201 ggtataaatg ctcctgccac ctcgagcctt ggcaggctat ttgacggttt tgcggttctt
     1261 gcaggattgg ggcagaaggc cacatacgag gcggagctgg ctgtggcgct tgaatcggcc
     1321 tatgatcctg gcgcaagacc ttttaatttt cctgtaaggg attttggaga ccatttcgag
     1381 gttgactgga ctgttgccct gaaacagggg atcgagaacc cagcttcaat atctggcgga
     1441 ttccacaagg gaataagtga agccatcgtg aaggggtgtt tgtttgccag gcaaaaaacc
     1501 ggcctgaaaa gaatttgtct cagtgggggc tgttttctaa accttatctt gctggagctt
     1561 gtttttgatg ccctttcaag cgaaggtttt gaggtgtata caaacattag tttgccgcct
     1621 ggcgacggtt gcgtcagttt tggccaggct gttgttgccc tttcaagggt tggaggtatg
     1681 cgatgtgtct agctgtacca gcaaagatat gcgaaaagaa cgggcttgat gccactgtcg
     1741 aattcggtgg aataaaaagg caggtaaggc ttgatctttt gccggatgcc gaggttggcg
     1801 agtttgtact tttgcacaca ggttttgcaa tagaaaaagt aaacaaggaa gaggcagaga
     1861 acatactcaa aacgattcag gaggtgtttg gtgacgtccc agtctgatgt tgccctcaag
     1921 ctccggggga tgatcgagga gcttgccgtc tccccaatga ccttgatgga ggtgtgtgga
     1981 acacacaccc atgcaatatt tgccaatggc atcagggcac tccttcccaa aacaataaag
     2041 atcatatccg ggcctggctg tcctgtttgc gtcacatcac agtctgatgt ggacaggatg
     2101 atatggcttg cctcgcaaaa gaatgttgta atctgcactt ttggcgacat gataaaagtg
     2161 cctggaaaag gtggctcgct tgcggttgca aggtcaatgg gttcaaacat aagggtcatg
     2221 tattctccct ttgatgtgat tgaatgggcc agacaggaaa aaggaaaaga atttgtcctt
     2281 gttggtgttg gttttgagac gaccacccct ggttttgcag caaccataaa acagtcaaaa
     2341 gcacttggta taaaaaactt gtccatactt tccctcaaca agactgttcc ggaggcactc
     2401 agggcgcttg cacagtctgg tgaggtgaag ctggatggat tgattttgcc gggccatgtc
     2461 tcagcgatca ttggcctcgg accttatgga ttcctggcaa aagaatttgg tgttcccggg
     2521 gttgtgaccg ggtttgaagg tttcgacatt ctttacggac tggccgttct tgtaaaaatg
     2581 atagcagaga aaaaacctgc aatagtcaat acttacacga ggtctgtcaa gccagatggc
     2641 aatccaaccg caatgtcact tatgggtgag gtttttgaca aatcacctgc agtctggagg
     2701 ggcatcggga caatcgaagg ctcggggctt gcattgtctg gtgaatatgc tggttttgat
     2761 gcggcaaaac ggtttgaaat gcccaatttt cctgatgaag aaccaaaagg gtgcctctgt
     2821 gggaaggtga ttacaggtgc agtcactccg gaggagtgcc cattgtttgg tgtttcgtgc
     2881 actccgtcag acccggttgg tccctgcatg gtttcaggtg agggcacatg ttcagcgtat
     2941 ttcaaattca gaaagggcct cagatgaaga gatttttgcc
//
LOCUS       DAOSRI010000213         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_29995_length_2978_cov_3.928840, whole genome shotgun sequence.
ACCESSION   DAOSRI010000213 DAOSRI010000000
VERSION     DAOSRI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29995_length_2978_cov_3.928840"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK717_06590"
     CDS             <1..462
                     /locus_tag="PK717_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47361.1"
                     /translation="NTVKLSPCPSFSVKRTGEDGDGKDGAGEVSFEFKNTREDGRDLV
                     LKGEVHLVSWGDDGKAPVFAKLNEPRHKLGPLLPGMLQEITFKLPEPPLLENNQKGEG
                     KYFALVVESNGLLDTKNSILSDFDKDPRPLFDGTPVALDQQKAIAVTVWGR"
     gene            591..>2978
                     /locus_tag="PK717_06595"
     CDS             591..>2978
                     /locus_tag="PK717_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47362.1"
                     /translation="MRKLVFSSSRVTSIILALVIFLAPFSFGRQNATASHLDESNQQI
                     LPPFLPSDCNALGGNFSIIGRDKYPSGLVMDRQLSEVTNIKETLNQAMMEYYDPYYGQ
                     TQIIDLPVKKPSSTPYPYNVLHRDFSWDTFFSSNPQIIKLQDKSFLIGEWCGYNSKLG
                     YAIRQEPLNDGQINLGFIDMDTGNMISSLKTPAVSSMVSVSKDIFWGEVGKLSGLVDV
                     KTMKVIYSPLYGPPVAFNDEYMASTQEIYSIRERKITANLSRLGIKASITKIKDDYVD
                     YIFNPVYPTPVKDPKPSLARVSFDGKLLFRKEFDFKIPVTINDTFEKNLLIMGRTEES
                     HILRYSICDIDTLETKHEFQIGENVFTVNNYAVAVSDPTRVATISNLIKIWGTNPVKL
                     VKVTHFPKEPIFLDAGGKIFFGYSMGDGQSQFYRLDGNCSPETFTNTIVDTMDQYAID
                     NGDIVGVSLASKKNEKGTFDTEIKKRNYKIGSKGNYYKELTIKTESFMENTGQFKIYD
                     RKIYVCTGFSSIECYDLNDGKLVASLEGIKTADWETTRTELLLENNVLTIVRYGTQKG
                     LLAYITCIDVQKMTRLYEAHVSAQEKANRPRIKVDYFDSSMIVLNNSSDTFINYFDGS
                     QSDVIKDSNFVTFDNGMLYLYCHNYSGEDSKKLLLFDVKTKNRKTIPFPEGAYQPQTK
                     FDDYFLFSGKLCTLGGDLVQSGLGSWLTLPRHGQAFHPILGITEKYVQPSATAYVPST
                     VAEIPNGLIGKLSPCPSFSVKRTGEGKDGLGEVSFEFRNTREDGRDLVLKGEVYLV"
BASE COUNT          943 a          588 c          673 g          774 t
ORIGIN      
        1 aataccgtca agctctcccc ttgcccatca ttctcggtca aaagaacagg ggaggatgga
       61 gatggcaaag atggagcagg tgaggttagc tttgagttca agaacacaag ggaggatgga
      121 agagatcttg tgttgaaagg ggaggtgcat ctggtctcct ggggggatga tgggaaggca
      181 cctgtgtttg ctaaattgaa tgaaccaagg cataagttag ggccacttct tcctgggatg
      241 ttacaagaga ttacttttaa gcttcctgaa ccacctttgc ttgagaacaa tcagaaaggt
      301 gaagggaagt attttgctct tgttgttgag tccaatgggc tactagacac aaaaaactcc
      361 attctctcag attttgataa agaccctaga cctctttttg atggtactcc tgttgctctt
      421 gatcagcaga aggccattgc cgtgactgtg tggggaaggt aggtaattga agacaattcc
      481 ttaaaccaga aaaaaacccg gagggtatta gaaacaggaa gtaagagtca caatgtagaa
      541 tcatgccaaa gcaaacaggc ttttgattgc tttgaaaatg gagaggggaa atgcgtaaat
      601 tggtcttttc ttcgtcaagg gtaacatcaa taattcttgc gctggtgata tttcttgcgc
      661 cattctcatt tggccgccaa aatgcaacgg caagccacct ggatgaatca aatcaacaaa
      721 tcctgcctcc attcttgcca tcagattgca atgctcttgg cggcaatttt tctatcattg
      781 gcagggacaa atatccatct ggcctggtaa tggatcgcca gcttagtgaa gtcaccaata
      841 taaaagagac actgaatcag gcaatgatgg aatattacga cccatattat gggcagacac
      901 aaatcatcga tctcccagtg aaaaagcctt caagcactcc gtatccttac aatgtgctgc
      961 acagagattt ttcctgggac acattctttt ccagtaatcc acagataata aagctacaag
     1021 acaaatcgtt cctgattggg gaatggtgtg ggtataattc aaaattgggg tatgctatcc
     1081 ggcaagaacc attgaatgat ggtcagatca atctcggctt cattgacatg gacactggca
     1141 acatgatctc cagtttgaaa acgcctgcag tcagttccat ggtcagcgtt tcaaaagaca
     1201 tcttttgggg tgaagttggc aaattatcag ggcttgtgga tgtcaaaacc atgaaagtga
     1261 tatattcccc cttgtatggc cctcctgtcg cattcaatga cgaatatatg gcttcaacac
     1321 aggagattta cagcataagg gagagaaaga tcacagccaa tctctccagg cttggaatca
     1381 aggcaagtat aaccaagata aaagacgact atgtcgacta catattcaat ccggtgtacc
     1441 caacaccggt aaaggaccca aaaccatctc ttgcaagagt ttcctttgat ggcaagctgt
     1501 tgtttagaaa agagtttgat ttcaagattc cagtgacaat caatgacact tttgagaaaa
     1561 accttctgat aatgggcaga acggaagaat cgcacatatt gcgctattca atatgcgaca
     1621 ttgatacgct tgaaacaaag cacgaattcc agattggtga gaacgttttt actgtgaata
     1681 actatgcagt tgcagtttca gatcccactc gcgtggccac aatctcaaat ctgataaaga
     1741 tctggggcac aaacccagtc aaacttgtaa aagtgacaca tttcccaaaa gagccaatat
     1801 ttctggatgc cgggggcaag atattttttg gctacagtat gggagacgga caatcacaat
     1861 tctatagact ggatggtaat tgctcaccag aaacattcac taacaccatt gtggacacaa
     1921 tggaccagta tgcaattgat aatggagaca tagtaggtgt tagccttgcc tcaaaaaaga
     1981 atgaaaaggg gacgtttgac acggagataa aaaagagaaa ctacaaaata ggctccaaag
     2041 gcaattacta caaagaattg actatcaaga cagaatcttt tatggaaaat accgggcaat
     2101 tcaagattta tgataggaaa atatatgttt gcacaggatt tagcagcata gaatgttatg
     2161 atttgaatga tggcaaactg gtcgcaagcc tggaaggcat aaaaactgcc gactgggaaa
     2221 caacaagaac tgaattgctc ctggaaaaca acgttttgac aattgtccgt tatggaaccc
     2281 aaaaaggttt gctggcctac atcacctgta tcgatgtaca aaaaatgacc cgcctttacg
     2341 aggcccatgt gagtgcgcaa gaaaaagcaa acagaccaag aataaaagta gactactttg
     2401 attccagcat gattgtattg aacaattctt ccgacacctt tatcaattat tttgatgggt
     2461 cacagtcaga tgttatcaaa gacagcaatt ttgttacctt tgataatggc atgttgtacc
     2521 tgtattgcca taattatagt ggggaggata gtaaaaaatt gctattgttt gatgtgaaaa
     2581 caaaaaatag aaaaaccatt ccattccctg aaggggcata ccaaccgcaa acaaaatttg
     2641 atgactactt tctttttagc ggcaaacttt gcacgcttgg aggagatctt gtccagagcg
     2701 gccttgggtc ctggcttact ctcccaaggc atgggcaagc ttttcaccca attcttggca
     2761 taactgaaaa atatgtccag ccttctgcaa ctgcatacgt tccatccact gtcgctgaaa
     2821 tcccaaatgg tttaattggg aagctttccc catgcccatc attttcagtc aaaaggacag
     2881 gagagggcaa ggatggatta ggtgaggtta gctttgagtt caggaacaca agggaggatg
     2941 gaagagatct cgtgttgaaa ggggaggtgt atctggtt
//
LOCUS       DAOSRI010000214         2963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30194_length_2963_cov_3.060523, whole genome shotgun sequence.
ACCESSION   DAOSRI010000214 DAOSRI010000000
VERSION     DAOSRI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30194_length_2963_cov_3.060523"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK717_06600"
     CDS             <1..582
                     /locus_tag="PK717_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR47363.1"
                     /translation="GQGTPLGRIIGSGAVVTGKVFGIERVPVVKGQAMPAYDPRAVHG
                     IGVTYATSPMGADHTAGYAIASNILGVGEKVDPLKPEGQVDLSRALQIATAFIDSSGM
                     CVFVAFALLDQPETNQALIDLVNAFYGWNWTADDTVEFGKRVLKMERDFNVGAGFTKE
                     HDRLPLFMKTQQLSPHNLTFQVKDEELDGVFNW"
     gene            726..1286
                     /locus_tag="PK717_06605"
     CDS             726..1286
                     /locus_tag="PK717_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47364.1"
                     /translation="MKAFFLLLQNGFSIKCLTGISIEQLLYNQLGLTPQLVEKKISTV
                     FLDGKPVDDIAMAIVQDGSSLALSGAMPGLVGATLRRKSPLASFRQSISVESGLGKSE
                     PIQGHVMVKLFNVLLKELGPFFLRTGIFVSAPAFVDLVSGKADDFLDKCIQVVVNGVS
                     VTLQSQGEWHSLLASHDSVHLKVKVG"
     gene            complement(1573..>2963)
                     /locus_tag="PK717_06610"
     CDS             complement(1573..>2963)
                     /locus_tag="PK717_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOR47365.1"
                     /translation="TISLMVMAEGFSRMGVPVFMADVKGDVAGLAMAGTANEKIQQRV
                     KEIGIDDYTNEANPVLFWDMYGKNGHPVRTTISEIGPGLLGRILEINDTQTGTLEIAF
                     KFADDQGLLLLDLDDLRALLSFVADNRKEVSAQYGLVNTQSIGAIQRALLTLEREGGQ
                     ALFGEPALELNDLMRTDLSGRGIISILNADQLILKPRLYSSFLLWLLSELFENLPEVG
                     DLDKPKLVFFFDEAHLLFDDAPPVLRQRVEQVVRIIRSKGVGVYFCSQFPDDVPNEIL
                     GQLGNRIQHALRAYTPRDQKAVRTAAETFVTNPKLNVTEVISQLAVGEALVSTLQDKG
                     VPMPVERTTICPPRCRMGTLTSEERSVVRARSPIGAKYDISVNRESAYEIINQRARNK
                     EAVQPETKGKEETKPVESGGKLGDLLWGSKRREGMVQAMAKQAARTVGSQIGRQIIRG
                     VLGGILGGSGRK"
BASE COUNT          756 a          746 c          678 g          783 t
ORIGIN      
        1 ggtcagggaa caccccttgg caggattatt ggaagcggcg cagtagtgac aggaaaggtt
       61 tttggcattg aacgtgttcc tgttgtcaag ggacaggcca tgccagccta cgacccgagg
      121 gcagttcatg gcattggcgt cacatacgca acaagcccca tgggcgctga tcatacagca
      181 ggatatgcca tagcctcaaa tattctgggg gttggtgaaa aagtagatcc tctcaaacct
      241 gaaggacagg tagacctttc aagggcactc cagatcgcaa cagccttcat cgattcatct
      301 ggcatgtgtg tctttgtggc ttttgctttg ctggatcagc cagaaaccaa tcaggcgttg
      361 atcgaccttg tcaatgcctt ctacggatgg aattggaccg cagacgacac agtcgaattc
      421 gggaaaaggg ttctgaagat ggaacgggat ttcaatgttg gagccggttt tacaaaagag
      481 catgatcgac tcccgctttt tatgaaaaca cagcaattgt cgccgcacaa tttaactttt
      541 caggtcaagg atgaagaatt agacggtgtt tttaactggt agacactttt gaagcttaat
      601 gaggaagggg caacatagcg caccttcctc atcttatttc aaaaagatac atggcaaacc
      661 aaaaccctca acacgatatc gacgcttcag ttctcaatat caatctgact gtccaacttt
      721 cagcggtgaa agcattcttt ctcttacttc agaatggctt ttccataaaa tgtcttaccg
      781 gcataagcat cgaacagttg ctctataatc aactgggatt aacaccacag ctcgttgaaa
      841 aaaagataag cacagtcttt cttgacggta agcctgttga tgatattgct atggcaatcg
      901 tacaggacgg ttcatcattg gcgctttcag gcgccatgcc cggcctggtc ggggcaacac
      961 tgcgtcgaaa gagccctctc gcttcattca ggcaatcaat aagtgttgag agtggcttgg
     1021 gaaagtcaga accaattcaa ggacatgtca tggtcaaact tttcaatgtt ctcctcaaag
     1081 agcttggccc ttttttcctc agaacaggta tttttgtttc agcccccgct tttgttgacc
     1141 tcgtatcagg aaaggctgat gatttccttg acaaatgcat acaggtagtg gttaacgggg
     1201 tgtccgtcac attacaatca cagggcgaat ggcattccct tctggcaagt catgattccg
     1261 ttcacttgaa agtaaaggta ggataatcca gatattttct ataagttccc tcgtcagcct
     1321 gtcaggcgac gaaaggcaac tatagtcttg ctgaaaccat gatcagcagt atgaggattc
     1381 ggatctccgc gtcacgtggc tcgatagtct ccctgatgct accacccttc agaatccagt
     1441 gttctgtgta atcctattcg ctaacttagg attgtatacc cacgaaccaa ttaaaatctt
     1501 gtagcacaga tttccagtgt ccgctaataa accatcccta ttaaaaatca gggtgataat
     1561 tggtgacgca acctatttgc ggccagaccc tccaagtatg ccgccgagta caccacgaat
     1621 aatttgccgt ccaatttgac tcccaacggt tctggctgct tgtttggcca ttgcctgcac
     1681 cataccctct ctccgtttgg agccccagag caaatcaccc agttttcctc cgctttccac
     1741 tggcttcgtt tcctctttcc cctttgtctc gggctgaaca gcttccttat ttctcgctct
     1801 ttgatttatg atttcataag cagactcacg attcacagag atatcgtact tggcgcctat
     1861 ggggcttctc gccctcacca ccgagcgttc ttcagacgtc aaggtcccca tccggcaacg
     1921 gggcgggcat atggtagtgc gctccaccgg cattggtaca cctttatcct ggagagtgga
     1981 aactaatgcc tcgccaaccg caagttgcga tatgacttca gtgacattca atttgggatt
     2041 ggtaacaaat gtttctgcag cagtccggac tgctttctgg tctcttggcg tgtaagctct
     2101 gagggcatgt tgaatgcggt tcccaagctg cccgaggatt tcgttcggta catcatcagg
     2161 aaactgtgaa caaaagtaaa ccccaacacc cttggagcga ataatccgta caacctgctc
     2221 tacccgctga cgcaatactg gtggtgcatc atcaaagagt aaatgggcct catcgaagaa
     2281 gaatacaagc tttggcttat cgaggtcacc tacctcaggg aggttctcga acagttctga
     2341 taaaagccac aggagaaagc tcgagtacaa tcttggtttc agaatgagct gatctgcatt
     2401 aagaatgctg ataatgcctc ttccgctgag gtcagtacgc atgagatcgt tgagttccag
     2461 agcaggttcg ccaaacagtg cctgtccccc ttcacgctca agggtcagca aggctcgttg
     2521 gatggcgccg atagactggg tattcaccag accatactgc gctgatacct ccttccggtt
     2581 gtctgctaca aaactgagga gagctctcaa atcgtcaagg tcgagcagta acaagccctg
     2641 atcatctgca aacttgaagg caatctcgag cgtacctgtc tgcgtgtcgt taatctcaag
     2701 gattcgaccg agcaggcctg ggccgatttc acttattgtt gtacggacag ggtggccatt
     2761 tttgccatac atatcccaga ataagaccgg atttgcttcg ttggtgtaat catcgatccc
     2821 tatctccttc acacgttgct ggattttttc attagccgtg ccagccatag caaggccagc
     2881 cacatcacct tttacatcag ccatgaacac aggcacgccc attcgtgaaa aaccctctgc
     2941 catgaccatg agagaaatag ttt
//
LOCUS       DAOSRI010000215         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30280_length_2957_cov_4.416954, whole genome shotgun sequence.
ACCESSION   DAOSRI010000215 DAOSRI010000000
VERSION     DAOSRI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30280_length_2957_cov_4.416954"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PK717_06615"
     CDS             complement(<1..715)
                     /locus_tag="PK717_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47366.1"
                     /translation="MRHLPRGMPLYEDLPTVFLNWEEMAQKLRSDHFSGYINVSSDSK
                     TGLILFREGRVTGSLFSSEAGDISRGQDALTKTTLAIQERKGTLSIYQTSADVCTMVE
                     WYFEGNQLYSPMESYFIDFPKFMETLGEKKLNGLVRIQSGDFSEFIYVDSGSVRGHFV
                     DGHPELQPASERLNETLSKKGTSVEIFKSSSGSQQNLEQVLTVVSKVVEPKPAPRIET
                     PPPPKQATPVIETKKPEAPK"
     gene            complement(805..2592)
                     /locus_tag="PK717_06620"
     CDS             complement(805..2592)
                     /locus_tag="PK717_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOR47367.1"
                     /translation="MPKEQFQRFDLRECSQFLSGSDALINLRWIAAGALFIASAAIWV
                     FLPSLWPFPLLIISALIVFFNIFLIYLRSSCPDGNRFFTLQVACDWIFLFLVCFWTGS
                     ISSPAVFLFPIYSILVGFFLPKKLAYLIAGFGSLLIGLLSLWVTSSPLAYVIGQAFFY
                     IIVLFFCTTIGIWASGQLREQLDLSDFLRKSLEKENSRLGQVYKSALAMNSGLDLPNV
                     LAIITKCFNETGLSNATVIRLLSEDGLTLNIASAEGISSEYAQRGSVALKDDEIDSEA
                     IKCKCPVFVSDVAKDERFLYKSEAERENLRSLLCIPLFSNEKAIGIIRCYTSDYHEFS
                     DEDVEFLSLLAAQASLAISNAVSYDKMVKLDNSRNAFIRMATHELRAPMAAVQSVLQI
                     ILDGYVGDVPENQKQLLSRSNNRITQLLRLVSELLELEKTTKTQDSFEPINLKELLSR
                     IISEIAPKADSKKVRLIMFTCGDNVIIKGIEEAIFRIFENLVDNAVKYTHSSGQVTIR
                     LLEDDGTATIQIVDNGIGIPAESIPKLFSEFYRAPNAKQEQVQGTGLGLSIVKKFVIM
                     HGGSISVSSQVDVGTTFTVTLPKDPQLTI"
     gene            complement(2594..2956)
                     /locus_tag="PK717_06625"
     CDS             complement(2594..2956)
                     /locus_tag="PK717_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR47368.1"
                     /translation="MVEAMRIALEAVGYKVSSAPNGTQGLKTLLEFAPDLIILDVMMD
                     TDTEGFNVAWAIRSDEDSSVYKKFKNIPILMVTSVSSEKKMKFDPKKDQEFLPVNDFI
                     EKPIRPQQLLQKVSQYLG"
BASE COUNT          811 a          644 c          674 g          828 t
ORIGIN      
        1 gttttggagc ctccggcttc tttgtctcta tgacgggtgt agcctgcttt ggtggtggcg
       61 gtgtttctat cctgggagct ggttttggtt cgacaacttt tgagacaaca gtcaaaacct
      121 gctccagatt ttgttgagaa ccactcgagc ttttgaagat ttcgaccgat gtgccttttt
      181 tagagagtgt ttcattgaga cgttcggagg ctggctggag ctccgggtgg ccatcaacaa
      241 aatggcccct caccgaaccc gaatcaacat atataaactc agaaaaatcg cctgattgta
      301 tccttacaag gccattcagc ttcttttccc caagggtctc cataaatttg gggaaatcaa
      361 tgaagtaaga ttccattggc gagtagagct gattgccctc aaaataccat tcaaccattg
      421 tgcagacatc tgcagaggtt tggtatatag acagagtacc ttttctttcc tgaatggcaa
      481 gcgttgtttt tgtcagtgca tcctggcctc ttgaaatatc tcctgcttca gacgagaaca
      541 aggaaccggt gacgcgccct tccctaaaga gaatgagtcc tgtttttgaa tcgctggaaa
      601 cattaatata tccggagaaa tggtcacttc tgagtttttg tgccatttcc tcccagttga
      661 gaaaaaccgt tggcaaatcc tcataaaggg gcattccacg tggtaagtga cgcatattac
      721 ctcctgttaa caagtaagtt tatcctaaaa taacatttgt acaatgtcaa gcggttcgca
      781 cctgattgaa tcggaatttt attattaaat tgtcagctgg ggatctttag ggagtgttac
      841 agtaaaagtt gtcccgacat ctacctgtga agatactgat atgctcccac catgcatgat
      901 gacaaatttt ttgactattg ataaaccaag accggtgcct tgcacctgtt cttgctttgc
      961 attcggggcc ctgtaaaatt cggagaagag ttttggtatt gactcagctg gaatcccaat
     1021 gccattatcg acaatctgaa ttgttgctgt gccatcatcc tcaagaagtc tgattgtcac
     1081 ctgaccactg ctgtgggtat atttcacagc attgtccaca aggttttcaa aaatcctgaa
     1141 aattgcttct tcgatacctt ttatgattac attgtctcca caagtaaaca ttataagcct
     1201 gactttttta ctgtcagctt ttggggcgat ttctgagatg atccttgaga ggagctcctt
     1261 cagatttatt ggctcgaagc tgtcctgggt ctttgttgtc ttctcaagct caaggagctc
     1321 tgaaaccagc ctcagaagtt gggttatcct gttatttgac ctggaaagaa gttgcttctg
     1381 gttttctggc acatcgccaa catatccgtc cagaattatc tgcaaaacgg attggacagc
     1441 cgccatgggc gccctcaact cgtgggtggc cattcttata aaagcgttcc tggaattgtc
     1501 gagtttgacc atcttgtcat aagagactgc atttgaaatg gcaaggcttg cctgggcggc
     1561 aagaagactt aaaaattcga catcttcatc agaaaattcg tggtaatctg aagtgtagca
     1621 ccttataatg ccaatggctt tttcattgct aaacaaaggg atgcacagca gggatctgag
     1681 attttcacgc tctgcttctg atttgtaaag aaatctctcg tcctttgcca catcactcac
     1741 aaaaacaggg catttgcatt tgattgcctc ggaatcaatc tcgtcatctt tcagggccac
     1801 agagcccctt tgggcgtatt cggacgagat tccttcagca ctggcgatgt taagggtcag
     1861 cccatcttcc gacaacaacc ttatcactgt ggcatttgaa agacctgttt cattgaagca
     1921 ttttgttatg atcgcaagca cattcggaag atcaaggcca gagttcattg cgagcgctga
     1981 tttgtacacc tggccaagcc tgctgttttc tttttcaagc gacttgcgca aaaaatctga
     2041 aagatcaagc tgttccctga gctgtcctga tgcccagatg ccaatggttg tgcagaaaaa
     2101 cagaactatt atgtagaaaa atgcttgacc aataacatag gccagtggag atgaagtgac
     2161 ccatagagag agaagcccga taagaaggga gccaaaacct gcaattaggt atgcaagttt
     2221 ttttggcaga aaaaaaccta caagaatgga atagatcggg aataaaaaca cagcagggct
     2281 tgaaatagag ccggtccaga aacacacaag aaaaaggaaa atccagtcgc aggcaacttg
     2341 aagtgtgaaa aatctgttcc catccgggca agaagacctc agataaatga gaaaaatatt
     2401 aaaaaataca ataagagctg aaattattaa gagcgggaat ggccacaagc tgggtaggaa
     2461 aacccatatt gcggcagaag ctatgaaaag ggcccctgcc gcaatccagc gaagatttat
     2521 cagggcatcg ctccctgaga gaaactgaga acattccctt aggtcaaacc tttggaattg
     2581 ttcttttggc actttagcct aagtactgtg acaccttttg aagcagttgc tgtggcctga
     2641 ttggtttttc gatgaaatcg ttgaccggaa ggaactcctg gtccttcttt gggtcaaact
     2701 tcatcttctt ctcagatgag acagatgtga ccatcaggat tggaatgttt ttgaatttct
     2761 tgtagacgga ggaatcctcg tctgatctga tggcccaagc gacattgaag ccttcagtgt
     2821 cggtgtccat catgacatcg aggatgataa ggtcaggggc gaactcaaga agggtcttga
     2881 gcccctgtgt gccattgggg gcggaagaaa ctttgtaacc aacggcctct aaggcgatcc
     2941 tcatcgcctc aaccata
//
LOCUS       DAOSRI010000216         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30351_length_2951_cov_4.207182, whole genome shotgun sequence.
ACCESSION   DAOSRI010000216 DAOSRI010000000
VERSION     DAOSRI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30351_length_2951_cov_4.207182"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PK717_06630"
     CDS             <1..845
                     /locus_tag="PK717_06630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOR47369.1"
                     /translation="ISDFLVKMIQDGCIQSLGLEESILSHSFYKIMSEKLPKTVLKSS
                     DGMCEAMRIIKSQDEIEFIRNSCKVACESFTETLGVVKEGVSERDICAELEYQMKRRG
                     AKKPSFDIIVASGFRAAMAHGVASDKKLKAGEFVVFDWGCFVNNYASDFTRTLVVGEP
                     TPKHIKIYDTVRRAQEAAMGKFKPGTKACDADKVARDIISNAGYEKNFNHSLGHGVGL
                     DIHERPGASMRTEDEFKAGMICTNEPGIYIEGWGGVRIEDTVLISDEGGVPLANLSKD
                     LIIV"
     gene            complement(842..1063)
                     /locus_tag="PK717_06635"
     CDS             complement(842..1063)
                     /locus_tag="PK717_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR47370.1"
                     /translation="MATAVKGKVVFIDERCKACGLCIEVCPTHILALSDNINQNGYHP
                     AYCVNMDQCIACKSCATICPDNVITVYRL"
     gene            1173..2285
                     /gene="buk"
                     /locus_tag="PK717_06640"
     CDS             1173..2285
                     /gene="buk"
                     /locus_tag="PK717_06640"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOR47371.1"
                     /translation="MSNSFYILAINPGSTSNKIGLFKGHDKVFELTIRHSREELAPFK
                     GIWDQYEFRYKTISEEFKKHPDTVGHLDAIVGRGGLIKPIVSGTYEVNEEMMQDAKNN
                     YQGEHASNLGCVLAHDLAKEYGCKAYIVDPTSTCEFQRLSFYSGHPLITRRAIAHALN
                     IHAIARKASQDLGIPYNKSRFIVVHCGGGITVASVVGGKMIDNNDATCEGPFTPERSG
                     SMPLIPFTNLCFSGKYTQSQIKKMIMGEGGFVAYLGTNSAVKAEEMAKSGDAAALEVY
                     QAMVYQIAKDIGSQATVQKGNYDAILITGGLAYSDYFIDSLSERISFLGKILRYPGED
                     ELGALAGGALRILEGSEKALSYPQFGERPPEWKFAT"
     gene            2267..>2951
                     /locus_tag="PK717_06645"
     CDS             2267..>2951
                     /locus_tag="PK717_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR47372.1"
                     /translation="MEICHLKKKLEEGVLTITIDRPAQLNALNKDVLAELEQVIDEAE
                     KNTSVRAIILTGEGKAFVAGADISTMVGMDETQALNFASFGQKVFDKIENLSKIVIAA
                     INGFALGGGCELAMSCDIRYASEKAKLGQPEVNLGVIPGFAGTQRLPRLVGPGKALEL
                     LVTGNVIDANQALQIGLVDKVLPPEGLMEEAIKLAKAIATKCPGAVSLNKKALKSGLP
                     NFNEKEEASL"
BASE COUNT          881 a          638 c          719 g          713 t
ORIGIN      
        1 cgatttcaga tttccttgtc aaaatgatac aggacggctg catccagtct cttggcctcg
       61 aagaaagtat tctttcgcac tctttttaca aaatcatgtc agaaaaactc cccaaaacag
      121 tcctcaaatc ttccgatggc atgtgcgaag caatgaggat cattaaaagc caagatgaga
      181 ttgaattcat aaggaattca tgcaaagttg cctgtgagtc tttcaccgaa acacttggcg
      241 tcgtcaagga aggggtctcg gagagagata tctgtgcaga gcttgaatac cagatgaaaa
      301 gacgcggtgc aaaaaaacca agcttcgata taattgtcgc ttccggtttc agggccgcaa
      361 tggctcatgg cgtcgcatca gataaaaaac tcaaggcagg cgaatttgtt gttttcgact
      421 ggggatgttt tgtaaacaat tatgcctcgg actttacaag aacactcgtt gttggagagc
      481 caacaccaaa acatattaag atatacgaca cggtaaggcg cgcccaagaa gcagcaatgg
      541 ggaaatttaa gccaggaaca aaagcttgcg acgccgacaa agtggcaagg gatataattt
      601 caaacgccgg ctatgaaaag aacttcaatc attctttggg gcacggggtt ggcttggaca
      661 ttcacgaaag accaggtgct tcgatgagga cggaggatga attcaaggct ggcatgatat
      721 gcacaaacga gcccggaatt tatattgaag gatggggtgg tgtaagaata gaagacaccg
      781 tcctcatcag cgacgagggc ggtgtcccgc ttgctaatct gtcaaaagat ctgattatag
      841 tttagagcct gtatacggtg atgacgttgt ccgggcaaat ggttgcacag gacttacagg
      901 caatgcactg gtccatgtta acacagtagg ctggatggta gccgttctgg ttaatgttgt
      961 cggagagcgc aagtatgtgt gtcgggcaaa cctcgatgca caacccgcat gccttgcacc
     1021 tttcatctat gaaaaccact tttcccttta cagctgttgc catttcaact cctttcaacc
     1081 acatgttagc ttgggcaagg ttgcatttca aggacagggt ggcatctttg acatgacaaa
     1141 acaaatattt acaatatatt tcggaggtac aagtgagcaa ttctttttac attctggcga
     1201 ttaatccagg tagcacttcc aacaagattg gccttttcaa gggccacgac aaagtgttcg
     1261 agcttaccat cagacacagt agagaagaac ttgcaccttt caaggggatc tgggatcaat
     1321 acgaattccg ttacaaaaca ataagcgaag aattcaaaaa acacccggac acagttggcc
     1381 accttgatgc tatcgtagga agaggcggtc tcattaagcc cattgtctct ggtacttacg
     1441 aagtaaacga agagatgatg caagatgcta aaaataacta tcagggagaa cacgcttcga
     1501 atcttggctg tgtactggcc catgatcttg caaaagagta tggttgcaaa gcatatattg
     1561 ttgaccctac ttcaacatgt gaatttcaga gactctcctt ttattctggc caccccctca
     1621 ttaccaggcg tgctattgca cacgccctta atattcacgc catcgccaga aaagcgagtc
     1681 aagatcttgg aattccttac aacaaatcaa gattcatcgt cgttcattgt ggtggcggga
     1741 taaccgtggc ctccgtcgtt ggcggcaaga tgatcgacaa caacgatgca acctgtgaag
     1801 gaccatttac acccgaaaga tcggggagca tgccgctcat accattcacc aatttatgct
     1861 tctctggcaa atatacccag tcgcaaatca aaaaaatgat aatgggtgaa ggcggctttg
     1921 tcgcctatct tggaacaaac tcagctgtca aagccgaaga aatggcaaaa tctggtgacg
     1981 cagcagcgct tgaagtctat caagcaatgg tttaccagat agcaaaggat attggttcac
     2041 aagccactgt tcaaaaaggc aattatgatg ccatacttat tactggcggt cttgcctact
     2101 cagattattt tattgacagc ctctcggaaa gaataagttt tctcggcaag atattgaggt
     2161 atcctggcga ggacgagctt ggagcactcg ctggtggtgc attaaggatt ttggagggct
     2221 cggagaaggc cctatcttat ccacaatttg gagaaaggcc acctgaatgg aaatttgcca
     2281 cctaaaaaag aagcttgagg agggggtcct cacaattacc attgacaggc ccgcacagtt
     2341 aaatgccctg aataaggacg tccttgcgga acttgaacag gtaattgatg aggcagagaa
     2401 gaatacgtcc gtcagggcaa taatactgac cggcgaagga aaagcctttg tggctggggc
     2461 tgacatttca accatggttg gaatggatga gacacaagca ctgaattttg ctagttttgg
     2521 gcaaaaggtt tttgataaaa ttgagaacct ctcaaagatt gtcattgccg caatcaacgg
     2581 ttttgcgctt ggtggaggct gcgaactcgc aatgtcatgc gacattaggt atgcaagcga
     2641 aaaagcgaaa ctggggcagc cagaggtcaa cctcggggtg atcccaggtt ttgcaggcac
     2701 gcaaagatta ccaaggcttg ttggacccgg aaaagcgctt gaattgcttg tcacaggcaa
     2761 cgtaatagat gccaatcagg ctcttcagat agggcttgtt gacaaggtgc tgccaccaga
     2821 ggggcttatg gaagaggcaa taaaacttgc aaaagcaatt gcaactaagt gcccaggggc
     2881 agtatcactc aacaaaaaag cgctcaaatc gggactcccc aatttcaacg aaaaggaaga
     2941 ggcctcactt t
//
LOCUS       DAOSRI010000217         2947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30419_length_2947_cov_2.423237, whole genome shotgun sequence.
ACCESSION   DAOSRI010000217 DAOSRI010000000
VERSION     DAOSRI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30419_length_2947_cov_2.423237"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PK717_06650"
     CDS             complement(<1..1324)
                     /locus_tag="PK717_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR47373.1"
                     /translation="MNNNQENFIGKLVGNTGQPDKLTIALRNSFSARRGEFVRIAHQE
                     RENDQNMDVLGRIVSLSRNSILFNPALGEGISEVELLPHSKVSGETVYGTIELVGYKD
                     PYTGEIRIPRRPLNPGTKVYSVDYDFLTKFYDFSEDTSIHLGNLVGYEKGSNVVPVYL
                     DANTLVTEHLAVLAMTGSGKSYTVGRVIERLVAQLNASVVVFDPHGEYGKALRGGTLN
                     FNDRLDSVEDVRDREKLVEIQKRLQALQDKGAGITAFTPQIRSFTEKYAGKNQNLALQ
                     LDHFDVDEFSGVLPGLTEPQQRVLDVALRYWKENFEEPRDIQELFSVLDDLDRLKNWV
                     AESGSPGEASALKGGSANIAAIRLRRMINEAQSFYTRAAGDPFDVYKMVGDDNEQYGR
                     LCIVDLQGLSNTARQVIVALISSELMKAAGDKNRPIRPSFIIYEEGHNF"
     gene            complement(1324..2469)
                     /locus_tag="PK717_06655"
     CDS             complement(1324..2469)
                     /locus_tag="PK717_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47374.1"
                     /translation="MKSVEMSRALTAAMSLRLPGTAGKLANKMRYVDSKGPLFHDGGL
                     IRNINQGKHEKVYAVGISSKSKSMPALGGLLYGAAALRHDLVVANNEVSEGLQDSRVD
                     INDMNFLGASERLVWKEFILVYRLLSDLMSSDTKPDIIFVDIPLLVSRAEQSVFLEEE
                     EVQEDWQPLLYVMEEFWQRHLNDFYPNNPDGPFLVSLQTKRDLNSAVLNAIREEGQSG
                     SPEAIDDKIVNLIREDWGQLRRVGILRFLKGTLKAGRRTAAFYYESLSKYMKRFEPKL
                     VSEHGLVGFHLQVGLRTPIWKVETIGKRDSWSTDSLDKLSGLISYLTIHDNPKMKPLP
                     LWFAEQLVRMPKEVLISYLKSTLAMLRDREVDQTWLEGIDTIQEEVE"
     gene            complement(2466..>2947)
                     /locus_tag="PK717_06660"
     CDS             complement(2466..>2947)
                     /locus_tag="PK717_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47375.1"
                     /translation="MGVDDNKGTILLKYQPKNTSGAVFITTLKLLSYSGMTNETDREE
                     LLKLLLTWENKQSLVNEEPTEDAASQDDSVLHAVAILSFSYGSKDPDKISAAMPRFFT
                     LNYDADTVRQALDQLSIYLTATKENWASKLEDYIDKAGHYSYAREIKEILEDEEADA"
BASE COUNT          725 a          752 c          674 g          796 t
ORIGIN      
        1 cgaaattgtg accttcttca tagatgatga aagagggtct tatcggacgg ttcttgtcgc
       61 ctgccgcttt catcaattca ctggatatca acgcaacgat gacctgtctc gcagtgttcg
      121 agagcccttg aaggtctaca atacataggc ggccgtattg ctcgttgtca tctccaacca
      181 ttttgtaaac atcaaaggga tcgcctgcag cgcgggtgta aaaactctgt gcctcgttta
      241 tcatcctgcg caaacggatg gcggcaatat tggcgctccc gcctttaagg gcgctggctt
      301 cacccgggct tcctgattcg gcgacccaat ttttcaagcg gtcaaggtcg tccaaaacag
      361 agaacaactc ttggatatcc cgaggctctt cgaaattttc tttccaatat ctcagtgcca
      421 catcaaggac acgctgttgc ggttctgtca ggcccggcaa cactcctgaa aattcatcca
      481 catcgaaatg atccagctga agagccaggt tctggttctt tccggcatat ttttcggtaa
      541 agcttcggat ttgaggcgtg aaggctgtta taccggcacc tttatcctga agggcctgaa
      601 gcctcttctg aatttcaacg agtttttcgc ggtctctgac atcctctacg gaatcgagcc
      661 ggtcattgaa gtttagggtt ccgccacgca aagccttccc atactccccg tgaggatcaa
      721 agactacgac cgatgcgttc aactgagcga caaggcgttc aataacgcga ccaacggtgt
      781 aacttttccc tgagccggtc atagccagca cggccaaatg ttctgtcacc agcgtattag
      841 catcaagata tacaggaacg acatttgagc ctttctcgta acccacaagg ttaccaaggt
      901 gaatactggt atcctctgag aaatcataga atttggtcaa aaaatcataa tcgacactgt
      961 agactttagt gcccggatta agcggtctgc gagggattct gatttcaccg gtataaggat
     1021 ctttgtagcc aaccagttca atggttccat acacggtttc cccagaaact ttggagtgag
     1081 gcagcagttc aacctcacta atgccttcgc caagagcagg attgaacagg atactgtttc
     1141 tggaaaggga cacgattcgc cccagtacat ccatgttctg gtcattttca cgctcctgat
     1201 gagcgatcct gacgaactcg ccgcgccttg ctgaaaagct gttccggaga gcaatggtca
     1261 acttgtcagg ttggccagtg tttccaacca gtttgccgat gaagttctct tggttattgt
     1321 tcattattct acctcttcct ggattgtgtc gataccttca agccatgttt gatcaacctc
     1381 tctatctctg agcatcgcaa gggttgattt caaatagctg atcagaactt ccttaggcat
     1441 acgaacgagc tgttcggcaa accaaagagg aagtggtttc atttttggat tgtcatggat
     1501 ggttaaatac gagatcagcc ccgaaagttt atcgaggctg tctgtggacc agctgtcgcg
     1561 cttaccaatt gtttctacct tccagattgg agtgcgtagc ccaacctgca ggtgaaatcc
     1621 gacaagacca tgctctgaaa caagctttgg ctcgaaacgc ttcatatatt tgctaagaga
     1681 ctcgtaatag aacgcagcag ttctgcgtcc agccttcaga gtccctttca ggaagcgtag
     1741 aatgcctact ctgcgaagtt gcccccaatc ctcacgaata aggttcacga ttttgtcatc
     1801 aatggcttcc gggcttcctg attgcccctc ttctcgaata gcattcagga cggcactgtt
     1861 cagatcacgc ttggtttgca gggacacgag gaatgggcca tcgggattgt ttgggtaaaa
     1921 gtcattcagg tgacgctgcc agaattcttc catcacatac aatagaggct gccagtcttc
     1981 ctgaacctcc tcttcttcaa gaaaaacact ttgctccgcc cgggaaacca gtagagggat
     2041 atccacaaaa atgatgtcag gtttagtatc agagctcatc aagtccgaaa gaagccggta
     2101 aacaagaatg aactccttcc acacaaggcg ttctgacgcc ccaagaaaat tcatgtcgtt
     2161 gatatcgact ctgctgtcct gcaggccttc actcacttca ttattggcaa caactaaatc
     2221 gtgccggagt gcagccgctc cataaagcaa accacccaaa gcaggcatgc ttttgctttt
     2281 cgaagaaatg ccaacggcat aaactttttc atgcttgcct tggttaatat tcctgatcag
     2341 gcctccgtca tgaaacagcg gccctttgga atccacatag cgcatcttgt tggccagttt
     2401 gcctgcggtg ccaggcaggc gcaggctcat ggctgctgtc aatgcgcgtg acatttcaac
     2461 gcttttcatg catcagcctc ctcatcttcc aggatttcct ttatctcccg cgcataggag
     2521 tagtgcccgg ccttatctat gtaatcttcg agcttagagg cccaattctc ctttgttgcg
     2581 gtcagataaa ttgaaagttg atctaaagcc tgccgtacag tatctgcgtc gtaattaagg
     2641 gtgaaaaaac ggggcatggc ggctgatatt ttatccgggt ccttgctgcc atatgaaaaa
     2701 gagagaattg ccacagcatg cagtacgcta tcatcctgag acgcagcatc ttcagtcggt
     2761 tcttcattga cgagcgactg cttgttttcc caagtcaaaa gcagtttaag cagttcctct
     2821 ctgtcggttt cattggtcat cccgctatag gagagcaact tcagggtagt tataaaaacc
     2881 gctcctgatg tgttcttggg ctggtattta agcaggatgg tgcccttgtt gtcgtcaaca
     2941 cccatca
//
LOCUS       DAOSRI010000218         2946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30426_length_2946_cov_3.932203, whole genome shotgun sequence.
ACCESSION   DAOSRI010000218 DAOSRI010000000
VERSION     DAOSRI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30426_length_2946_cov_3.932203"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PK717_06665"
     CDS             <1..1196
                     /locus_tag="PK717_06665"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47376.1"
                     /translation="HPRLGLGWRGMGFDLDSEKIKEGLGTLGIDIKVGDPLLATIPGF
                     RDNDLRDEIDLIEEAIRVIGYDTIPTTFPIIEEKIVTETPIFLLERRLKKILSGMGLY
                     ECVSKPMTCEDELKLMKLETLVPNAPKVLNPMTTDSTIMRPVCELGVLAASSYNARQG
                     NSTMALFEVGSQFGPECRRLVMIMTGKRPSGWDSQLKAKETEGKKDNIENYDFFDLKG
                     SLEALFDLLCVCGVSYRPLNKPHPHLHQYRQAEIAISGNPIGYIGQLDPEVNERLQVA
                     TPITIANINIENLLPHVPEVIVAKPVPRFPMVLRDIAVLAPETVPCSQIESTIKDHAG
                     NNLSSIRLFDQFVGGFAREGKRSLAFSLSFYDPEGTLLGSQIDAIIEKIDLALEKIGA
                     SVRKA"
     gene            1193..2872
                     /locus_tag="PK717_06670"
     CDS             1193..2872
                     /locus_tag="PK717_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR47377.1"
                     /translation="MNSWELAEKLNFLARLARYTNTGIGSNQFLDASTTIRHLDVDEV
                     ELQMVGFLLAQGLLSETTASAISEMIKTGSSSLEQALLKKTPKGVQSLLQISGLSISN
                     IARLYEDCGIETLEELRKASRGGLLSSRKGFGERFSKQVEGMVTQTLSGRRTLPLSNA
                     IHLANLLQRKVKHFEEASKVFVAGDIRRGRELIEKIDLLVVVSKPTVKTAQEISKYLE
                     FGECIITKYGCEGQVSGVQVRVHLCRQEYTGSAMCLATGPGDHYQKLCSRTAFKNRSV
                     AGFLLVKGDEESFYKQLGLPYIPPEIREWPDCIELSEGKLGWDIVEEDDILGDLHAHT
                     PWSDGRSRLSELVRQAEQMGLEYLTVTDHAQGISVTKGLSAQDLEAQTDKIRKMNAFK
                     GTVKIVPGIEYNIDADGHVDFQTRQKIMRIGALHSGLGDDPSILENRYLRAISSGAID
                     IVAHPTLRRLLVRPPIEIDWDMIFSECSKAGVAVELNYAPDKLDPPWQVARIAMKHGC
                     MFALGSDAHYALHLKELPSGVKLARRAGVSRENLLNCKSYEQVRELCWRGQ"
BASE COUNT          848 a          667 c          735 g          696 t
ORIGIN      
        1 gccacccgcg ccttggcctc ggatggcgtg gcatgggttt tgatcttgat tcggagaaaa
       61 taaaagaagg ccttggcacc cttggaattg acatcaaggt tggagatcct ctattggcca
      121 ctatcccggg gttcagggac aatgacctca gggacgaaat cgaccttatc gaggaagcaa
      181 taagagtcat aggatatgac acaatcccca ccacttttcc aataatcgag gagaagatag
      241 tcaccgaaac cccgattttc cttcttgaga gaaggctcaa aaaaatcttg tccggaatgg
      301 gcctttacga gtgcgtctca aaaccaatga cctgcgagga cgagctcaaa ttgatgaagc
      361 tcgaaacgct tgtcccaaac gccccgaagg tcctcaatcc gatgacaaca gattcaacaa
      421 tcatgaggcc tgtttgcgag ttgggtgttc ttgcggcatc ttcatacaac gccagacagg
      481 gcaattctac catggcgcta tttgaggtgg gttcacagtt tggacctgaa tgccgcaggc
      541 tggtcatgat tatgaccggc aaaaggcctt ccggatggga ctcccagctc aaagccaaag
      601 aaaccgaagg caagaaagac aatatcgaga attatgactt ttttgacctg aaaggctcac
      661 ttgaggcgct gtttgatctg ctttgcgttt gtggtgtctc gtacagacct ttaaataaac
      721 cccaccccca cttgcaccag tacaggcaag ctgaaatagc aatctctggc aacccaatag
      781 gctatatcgg ccagcttgac ccggaagtaa acgagagact gcaggttgca actcccataa
      841 caattgcgaa cataaacata gaaaaccttc ttccacacgt tccggaagtg attgttgcaa
      901 aacctgttcc gaggtttcca atggtcctga gggacatagc ggttcttgca ccggaaacag
      961 tcccttgctc ccagatcgaa tcaacaatca aagaccacgc cggcaacaat ctttcatcga
     1021 taaggctttt tgaccagttt gttggcggat ttgcaagaga gggcaagcgt tcgctggcat
     1081 tctcgctgtc tttctatgat cccgaaggga cgctccttgg cagccagatt gacgctataa
     1141 tcgagaaaat tgatctggcc ctagaaaaaa ttggtgcttc tgtcagaaag gcatgaactc
     1201 ttgggagctt gcggaaaagc tcaatttttt ggcaaggctt gcaagataca caaatactgg
     1261 tattggctcg aaccagtttc ttgatgcctc gacaacaatc agacacctgg acgtcgatga
     1321 agtcgaactc caaatggtcg gtttcctttt ggcgcaaggg ttgctttctg aaacaacagc
     1381 ttctgcaatt tcagagatga taaagactgg cagttcaagt cttgagcagg cattgctgaa
     1441 aaaaactcca aaaggcgtgc agagtcttct gcagatttca ggccttagta tctcaaatat
     1501 cgcaagactc tatgaggatt gtggaataga aacacttgag gagctcagaa aagcatcaag
     1561 aggtggtctg ctttcctcaa gaaaaggttt tggtgagaga ttttcaaaac aagtagaggg
     1621 aatggtgacg cagaccctct ccggaaggag gaccctcccg ctttcaaacg caatacatct
     1681 ggcaaatctt ctgcaaagaa aagtaaaaca ttttgaagag gcgtcaaaag tatttgttgc
     1741 tggcgacata aggcggggta gggagctgat agaaaaaatt gatctgcttg ttgtcgtttc
     1801 caagccaacc gtaaaaacag cacaagagat atcaaaatat ctggaatttg gtgagtgcat
     1861 cattacaaaa tatggctgcg aaggccaggt ttcaggtgtc caggtgagag tacatctgtg
     1921 cagacaagag tatactggct ctgcaatgtg ccttgccact ggccccggcg accactacca
     1981 gaagctctgc tcgaggacag cattcaaaaa cagatcagtt gctggttttt tgcttgtaaa
     2041 aggtgacgaa gaatctttct acaaacagct tggtttgcca tacattccgc cagaaatacg
     2101 cgaatggccg gactgcatag agctttccga aggcaaactc ggctgggaca tcgttgaaga
     2161 agatgacatc ctgggcgatc ttcacgcaca tacgccatgg agtgatggca gatcaaggct
     2221 ttcggagctt gtaagacagg cagagcagat gggtcttgaa tacttaacag tcactgatca
     2281 cgctcaagga atatctgtaa caaaaggact ttcagcgcaa gaccttgagg ctcagacaga
     2341 caaaataaga aaaatgaacg ctttcaaagg aacggtaaag atagttcccg gcattgaata
     2401 taacattgat gccgatggcc atgtcgattt ccagacaagg caaaaaatca tgaggattgg
     2461 agcactacac tccgggctcg gtgacgatcc atccatcctg gaaaaccgct atttgagggc
     2521 aataagctct ggtgcaattg atatcgtggc gcacccgacg ctccggagac tccttgtcag
     2581 gccacctata gaaatagact gggacatgat tttttcggaa tgttcaaaag caggtgttgc
     2641 agtggagctg aattatgctc ctgacaagct tgatcctccc tggcaggtgg caaggattgc
     2701 catgaaacat gggtgcatgt ttgcgcttgg aagtgatgcc cattatgcat tgcacctgaa
     2761 agaactgcct tcaggggtga agcttgcgcg aagggctggc gtttcccggg aaaatttgtt
     2821 aaactgcaaa tcatatgaac aggtccgcga gctctgctgg aggggtcagt gaacgatcat
     2881 gcccttaggg tgcttgagtt tgagaaaata aaagagaaac tcaccgaatt tgcgcacacc
     2941 cagggt
//
LOCUS       DAOSRI010000219         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30513_length_2940_cov_3.386135, whole genome shotgun sequence.
ACCESSION   DAOSRI010000219 DAOSRI010000000
VERSION     DAOSRI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30513_length_2940_cov_3.386135"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK717_06675"
     CDS             complement(<1..336)
                     /locus_tag="PK717_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOR47378.1"
                     /translation="MNVRIIPRLDIKGPNLVKGIHFEGLRVLGKPEYFARYYYENGAD
                     ELIYLDAVASLYGRNSLIDIIEKTAREIFIPLTVGGGLRSVDDIRTVLRAGADKVAIN
                     TAALACPDLI"
     gene            complement(333..986)
                     /locus_tag="PK717_06680"
     CDS             complement(333..986)
                     /locus_tag="PK717_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOR47379.1"
                     /translation="MNDSCVIIGGGGHAKVLIDCLTLINEVRIEGVLEKNEIQWGRKI
                     LDVPVLGGDDLIDEMLIRGVRYFVVGVGGTGDNRIRQRLFEFGLHKGLEPMTIIHPAA
                     IVSSWAMLKSGCQILSGAIINPGALLERNAIVNSGAIVEHDCVIGEHAHIATGAKLGG
                     GVRIGAYAHVGLGASIRQYINVGEYALIGAGAVVTRDVPKSQTVTGSPARPLQKKSA"
     gene            complement(983..2164)
                     /gene="neuC"
                     /locus_tag="PK717_06685"
     CDS             complement(983..2164)
                     /gene="neuC"
                     /locus_tag="PK717_06685"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOR47380.1"
                     /translation="MRKIGLVSVGRSDYGIYLPVLKAIHADPDLELLLFVSGMHLAPG
                     FGLTVDAIVSDGFKIAAKVEMLLASNTPEGMAKSIGLGIIGYAQSFVENRPDILVVLG
                     DRFEMYAAVVAALPLRIPVAHIHGGEVTEGAIDDALRHSMTKMSHLHFVSNREYARRV
                     EQMGEEEWRITVSGAPSLDNLNSVPLLSLKEIEERYNIRFDHPPLLVTFHPVTLEIDQ
                     VEYQIDELLAALHECGMPTIFTMPNADAGNAIIRRKVVEYEKEHPKTWMLENLGTQAY
                     FSLMRYSALMVGNSSSGIIEAPSFILPVVNIGTRQKGRARAANVIDVGYSRNEIISGI
                     YKGLSGFFRDSLSGLKNPYGEGLAAEKIVTRLKTVNIDSALVRKHFCDLPASFPGVKR
                     Q"
     gene            complement(2161..>2940)
                     /locus_tag="PK717_06690"
     CDS             complement(2161..>2940)
                     /locus_tag="PK717_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOR47381.1"
                     /translation="YEEFDKIKAYCRKKKIVFLSTPFDRDSADFLDDLGVSAFKISSG
                     EITNYPLLQHIASKGKPIILSTGMSYLGEVEKALEMIYKCNNRKIILLHCVSNYPAAP
                     MDVNLRAMKTMSEAFRVPVGYSDHTLGIEVSLAAVALGACVLEKHLTLDRKLIGPDHE
                     ASLEPNELRSLVKGVRMIESAMGNGCKIPSPREENILMTARRSLVAASDIPAGALISN
                     EHIAIKRPGTGLPPVMISHLIGRKVRQAIKADTLLELDMFS"
BASE COUNT          795 a          794 c          594 g          757 t
ORIGIN      
        1 gatcaaatca ggacatgcaa gggccgcggt attaatggct actttgtcgg cgcctgcacg
       61 tagaacggtg cgaatatcgt cgacactgcg taaaccaccc ccaaccgtca gggggatgaa
      121 tatttctcgt gccgtttttt caatgatgtc gatcagactg ttacgcccat acaagcttgc
      181 caccgcatcc aaatatatca attcatcagc gccattttca taataataac gggcaaaata
      241 ctcaggctta cctaaaacgc gtagaccttc aaaatgaatg cctttgacaa gattaggccc
      301 cttgatgtcg aggcgaggaa ttatgcggac attcatgctg acttcttttg caatggacgg
      361 gcaggagatc cggtcactgt ttgactcttt ggcacatctc tggttacaac ggcgccggca
      421 ccgataagag catattctcc cacatttata tattgccgga tggaggcgcc cagccccaca
      481 tgtgcataag ctcctatgcg aacccctcct cccagcttcg caccggtggc tatatgggcg
      541 tgctccccga tgacacaatc atgctccaca atagcaccac tattgacaat tgcattacgc
      601 tccaataatg ctcctggatt gataattgct ccggaaagaa tttgacatcc ggatttaagc
      661 atggcccagg aagatactat cgccgccgga tggattattg tcatcggttc gagaccttta
      721 tgtaaaccaa actcaaaaag acgctgccta attctgttgt cacccgtacc gccaacgccc
      781 acgacaaagt agcgtacacc tcttataagc atttcatcta tcaggtcatc tcccccgagt
      841 accggcacat cgagtatctt tctgccccac tgaatctcat ttttctctaa tacgccctct
      901 atacgaactt cgttaatcaa tgtgaggcag tcgatcaata ctttggcatg gccgcctcca
      961 ccaatgatca cacatgagtc attcattgtc gttttacacc cgggaaggac gccggcaaat
     1021 cacaaaaatg cttccttacc aaagcgctgt ctatgtttac ggtcttaagc ctcgtgacga
     1081 tcttttccgc ggcaaggcct tctccatacg gatttttcaa cccactcaaa ctatcccgaa
     1141 aaaaaccaga caaaccttta taaatgccag agatgatctc attccgggaa taacctacat
     1201 cgatgacatt agccgccctt gctcgtccct tttggcgtgt tcctatattc accaccggca
     1261 gaataaaaga cggtgcttca ataatcccac tggatgaatt tcctaccatt aaagccgaat
     1321 aacgcatcag actgaagtat gcctgggtgc caagattttc cagcatccag gtttttggat
     1381 gctctttttc atattcaact accttccgac gaattatcgc attgccggca tcagcgttcg
     1441 gcatggtaaa gatcgtcggc atgccacatt catgcaaagc cgccagcagt tcatcgatct
     1501 ggtattcaac ttgatcgatt tcaagtgtca ctggatgaaa ggtgactaaa agcggcgggt
     1561 gatcgaatcg gatattataa cgttcttcga tctccttcag agagagaaga ggcacggagt
     1621 tcagattatc caaactggga gcaccggata cggttatacg ccattcttcc tcccccattt
     1681 gttcgactcg cctggcatat tcccgattag acacaaaatg caagtgactc atctttgtca
     1741 tggaatgacg aagcgcatcg tcaatagccc cctcagtgac ctcgccgcca tgaatatgag
     1801 ccactggaat ccgtaacgga agtgcggcaa ccacagcggc atacatttca aatctgtctc
     1861 ccaaaacaac caatatgtcg ggacgattct cgacgaaaga ctgggcatag cctataatgc
     1921 ccagtcctat ggatttggcc ataccctccg gtgtgtttga cgccagcagc atctccactt
     1981 ttgccgctat tttgaaacca tcacttacaa tcgcgtctac cgttaaacca aatccaggcg
     2041 cgagatgcat gcccgaaaca aacaatagca gttccaagtc cggatcggca tgaatcgcct
     2101 tgagaacggg caggtaaatt ccatagtccg aacggcctac gctaaccaag cctatctttc
     2161 tcatgaaaac atatccaact ccaataatgt gtccgcttta atcgcctgac ggactttcct
     2221 gccaataaga tggctaatca tgacaggagg caaacccgta cccgggcgtt tgattgcgat
     2281 atgttcattg cttatcagcg ccccggccgg aatgtcactg gcggcgacga gactgcggcg
     2341 agccgtcatt aatatatttt cttcccttgg cgacggtatt ttacatccat tgcccatagc
     2401 cgattctatc atccgaacac ctttaacgag ggatcttaat tcattgggct ccaaagaggc
     2461 ttcatgatct ggaccaataa gtttacgatc cagagtaagg tgcttctcca agacacaagc
     2521 ccctaaagcg accgccgcca gtgaaacctc gatgcctaat gtatgatcgg aatatccaac
     2581 tggaaccctg aaagcctccg acatggtctt cattgcccgt agattcacat ccatcggagc
     2641 cgctggataa ttagacacac aatgcaataa aattattttt ctattattgc acttatatat
     2701 catttccaag gccttctcaa cctctcccaa gtaagacata ccagtggaca atatgatcgg
     2761 tttacctttg ctcgcaatgt gctgcaataa cggataattt gttatctcac cagaggatat
     2821 cttaaaggcc gatactccca gatcatccag aaaatccgca ctgtcacggt cgaacggggt
     2881 tgataaaaaa actatcttct ttttccggca ataagctttg attttgtcaa attcctcata
//
LOCUS       DAOSRI010000220         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_30589_length_2935_cov_3.557986, whole genome shotgun sequence.
ACCESSION   DAOSRI010000220 DAOSRI010000000
VERSION     DAOSRI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30589_length_2935_cov_3.557986"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PK717_06695"
     CDS             <1..1031
                     /locus_tag="PK717_06695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR47382.1"
                     /translation="LVMYGKSSAKTSGLSDKVKSMLSSSGLKVTEFFGVEPNPRDDTC
                     DRAASLGRQAGCDAVIAVGGGSVMDAAKLTSACIVSGKPTWDYVTRVYPVEKALPIIM
                     VPTLAATGSDFNSGAVITNWTMKQKFGAYSPLFFPTVSIIDPVLTTTCNRNVTAWGGV
                     DIMIHVLETYLGTNDAHCPIPDYFSEGIVKTVMKYLPLALADGNDIVARTQLSWASAV
                     ALSGMPNGGRPGGFLLHWMEHVMSAHFDIPHGLGLAYLLVPTLSYFKENFPERHYMFT
                     NNLMPDPAGFLESIGCDTTLEKLGIPGSSVPTMVDDLFVQKGKGDEMPGYPSFDKKWA
                     EKIYKGRF"
     gene            1038..>2935
                     /locus_tag="PK717_06700"
     CDS             1038..>2935
                     /locus_tag="PK717_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47383.1"
                     /translation="MLRKCCRQGVALFLVMLLSFVFTPVDVRGSVRESVETTVQRLTA
                     WKLEKVATTCTADLRKMVIQGPSKELVLLDIEPSMLRVTRISTLFQPGDLAGPKTYPG
                     TIKVMSEPQLPKRGDHVGFIGMSSGRRYLVMLNLNGKSAKTAMVPDTTYDGIKVKWDL
                     LWFRLNADGSRFIGVCLGKIVTSGKSGSCISLVSMGTNGAGLFFLSRPRWDGKKFYYP
                     KTGTGKILSPIGIARETGQVFFKAEIGQSGKHGVYTCSSDGKNFKLLITATTRYSITA
                     TVSSVSSTDLVTDDGKYLVLNDNGMEHPGAVTFEIATGDVKAKLTHKSVGVSCDGKFS
                     FFSDPTGFGMEFIFGGEPYVLIEPNQPNWPVVGKINNRSNSLEPSFGNITCSPFGFLG
                     RSETDAVAVGEVYFVGIPNPPVPPTPKLVVPPVIDFGLVDGNSSLSLPVGNEAKTAII
                     GSATIKTTTESCNPFSFSGEQSADFSSIGDIPVSLNTTCMSKGFTYEAEVLVSSIAGK
                     STVQMIATLNDSDRLLVRLGIGRKTSWIGSQVSELPVAPFILGGVTMVPLRVFLNALP
                     CDFEWSAEDQLAKINFQDKNIEITIGKEKMLVNGQEVPMAKPAMIKDGQTFLPLRSVT
                     EVFDGTLG"
BASE COUNT          795 a          600 c          727 g          813 t
ORIGIN      
        1 cacttgtgat gtatggaaaa tcgtctgcca aaacctcagg gctctctgat aaagtaaaat
       61 ccatgctttc ttccagtggg cttaaagtaa ctgagttctt tggtgttgag ccaaacccaa
      121 gggacgacac ttgcgataga gccgcaagcc ttggcagaca agccggttgc gatgcagtca
      181 tagctgttgg tggcggttct gtcatggatg cggcaaaact gacatcagcg tgcattgttt
      241 caggaaaacc aacctgggac tatgtcacga gggtgtatcc tgtagaaaaa gctttgccaa
      301 taatcatggt gccgactctt gcggcaacgg gctctgattt caatagcggt gcggtaataa
      361 ccaactggac aatgaagcag aagtttgggg cctattcgcc actgtttttt cctactgttt
      421 caataataga ccctgtttta actacaacgt gtaatcgtaa tgtgacggct tggggtggtg
      481 tggacataat gatacacgtt cttgaaacat accttggaac gaatgatgcc cattgcccca
      541 tacctgacta ctttagcgag ggcatcgtaa aaacagtcat gaagtacctt cctcttgcac
      601 ttgctgacgg aaatgatatt gtagcaagaa ctcagctttc atgggcttct gcggttgcac
      661 tctccggaat gccaaatggt ggaaggcccg gtggctttct gctccattgg atggagcatg
      721 taatgtcagc acactttgac attccgcatg gtcttgggct ggcttatctg cttgttccca
      781 cactctcata tttcaaggag aatttcccgg aaagacacta tatgttcaca aacaacctca
      841 tgcctgaccc ggcaggattc cttgaaagca ttggctgtga cacaacgctt gagaagcttg
      901 gcatccctgg atcatcagtc ccaaccatgg ttgacgacct ttttgtgcag aaaggcaaag
      961 gggacgaaat gccaggttat cctagttttg ataaaaaatg ggctgaaaag atatacaaag
     1021 ggagatttta agttctgatg ttgcgtaaat gttgtcgtca gggagttgct cttttcttgg
     1081 taatgctgtt gtcgtttgtt tttacccctg ttgatgtgag ggggagtgta agggaatctg
     1141 tagaaacaac agtacaaaga cttacagcct ggaagcttga aaaagttgcg acaacatgta
     1201 ccgccgattt gagaaagatg gtcattcagg ggccttcgaa agaacttgtt cttctggata
     1261 tcgagccctc catgttgagg gtgacaagga tttcgacgct tttccagcca ggtgatcttg
     1321 ccggtccaaa aacatatccg ggaacaataa aggtcatgtc agaacctcaa ttaccaaaaa
     1381 gaggggatca tgttggattt atcggcatgt cttctggcag aagatatctt gtgatgctca
     1441 atctgaatgg caaatctgcc aaaacggcca tggtccccga tacaacctat gatggaataa
     1501 aggtgaaatg ggacctgctt tggttcagat tgaatgctga tggatcaaga tttattggtg
     1561 tctgtctggg gaaaatcgta acctctggaa agagtggctc ctgcatttcg cttgtttcaa
     1621 tgggaacgaa tggcgcagga ttatttttcc ttagcaggcc aagatgggat ggaaaaaaat
     1681 tctactatcc aaaaactggc acgggaaaga tattgagtcc gataggtatt gccagagaaa
     1741 cagggcaggt tttctttaag gctgaaattg gccagagtgg caaacatggt gtgtatacct
     1801 gttcttcgga cgggaagaac ttcaagcttt tgattactgc aacaaccaga tactcaataa
     1861 cagccaccgt gagcagtgtt tcttctaccg atcttgtcac agatgacgga aaatatcttg
     1921 ttctcaatga taatggaatg gaacacccag gtgctgtaac tttcgaaatt gcaactggag
     1981 atgtcaaggc aaagctcacc cataaatcgg ttggggtatc atgcgatggg aaattttcct
     2041 ttttctctga tccaaccgga tttggaatgg aattcatttt tggaggggag ccatatgtgc
     2101 ttattgagcc taaccagcca aattggccgg ttgttggcaa gataaacaac aggagcaatt
     2161 cccttgagcc gtcctttggt aacatcacct gttctccgtt cggttttctt gggagatcag
     2221 aaactgatgc tgttgctgtt ggcgaggttt attttgtggg aattccgaac cctcctgttc
     2281 caccaactcc aaaacttgtt gttccaccag tgattgattt tggccttgta gacggcaatt
     2341 cctccttgtc gcttccggtt ggaaatgagg ccaagacggc cattataggt agcgccacga
     2401 tcaagacaac aactgaatca tgcaacccat tttcgttttc tggagagcag agtgccgatt
     2461 tttcttcaat aggcgatata ccagtcagcc tcaatactac ttgcatgagc aagggtttta
     2521 cgtatgaagc tgaggtattg gtcagttcga ttgctggaaa aagcactgtc cagatgattg
     2581 caaccctgaa tgattctgat aggttgcttg ttcggttagg aatcgggaga aagacttcat
     2641 ggattggtag ccaggtgagc gaattgcctg tggcaccctt cattttagga ggagtgacca
     2701 tggtaccatt gagagtcttc ctaaacgctc ttccatgcga ttttgagtgg agtgctgaag
     2761 atcagttggc caaaataaat tttcaggata aaaatattga aataacaatt ggaaaagaaa
     2821 aaatgcttgt taatggtcag gaagtaccaa tggccaaacc ggcaatgata aaggatggtc
     2881 aaacttttct ccctttaaga tcagtaaccg aggtttttga cgggacactg ggctg
//
LOCUS       DAOSRI010000221         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_31485_length_2872_cov_4.517927, whole genome shotgun sequence.
ACCESSION   DAOSRI010000221 DAOSRI010000000
VERSION     DAOSRI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31485_length_2872_cov_4.517927"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2617)
                     /locus_tag="PK717_06705"
     CDS             complement(197..2617)
                     /locus_tag="PK717_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOR47384.1"
                     /translation="MNWSNLDEKTQRIFYLAQDEAYELGNDYLGTEHLLLGLIRENTS
                     FAMQTLRELEIDFQGLRDRIVSLVRGVRNSAIGPVQKSHIVLSERAKRALDLAEKEMK
                     AQSSTKLTPEHLLIGILSEAEGLAARILRDMDIDLDRFRSAVDESVEEHGHDKKGKKS
                     LVEQFSRDLTALARRGKLDPTVGRMVEIQRITQILSRRTKNNPVLVGDPGVGKTAIVE
                     GLAQRIVDGEVPETLRDKRVLALDLAAVIAGTKYRGEFEERLKGILDEIDHSQGKIIL
                     FIDEIHTIVGAGAAEGAIDASNILKPALARGELQCIGATTMNEYRKSIEKDAALERRF
                     QMVQVEEPTVEETVKILIGLAPRYENYHKVTIQQTALEAASKLSAQYIQGRFLPDKAI
                     DLIDEACAKVRLSVMNLPESIREMETTKRSLRKEKQSAAESQEFEKAAQLRDKEREIE
                     DQLRIKREEWQKDVDKLSLVVTAEDIADVVSQWTGIPTTRLLQQERERLLTLEKVLHE
                     RIIGQDEAVVAVSKALRRARVGLAGRNRPIGSFVFVGPSGVGKTELARVLANHLFGSE
                     KALLRIDMSEYMEKHAVSRLVGAPPGYVGFEEGGQLTEKIRRKPFSVVLFDEIEKAHP
                     DVFNILLQIMDDGRLTDSQGRLVDFRHSIVIMTSNIGSQWKNTKSLGFAGADTEDRDD
                     EKEGDRLDSEIKKVFRPEFLGRIDGIIRFKPLTKEELCQVARLLINKLSETLTDEGLK
                     VDLTDNAIKALVEKSYDQKLGARPLVRAIQNLIEDPIAEKTLAGEFSSGDTIKIDQNG
                     NGLTLSKS"
BASE COUNT          649 a          724 c          627 g          872 t
ORIGIN      
        1 tactgccagt gctgcaaggg acacgtcaag aaactgcttg agtccgcttt gaagcgcgaa
       61 ctgaaggcag acattgtcca gacggcctgc tctggcggaa aaacatgcag ctttgttgta
      121 tatctagatt aaataatccc ataaaaacaa aaatcctcaa acctagtggg tttgaggatt
      181 tttaaatttg agttgtttat gacttggaaa gtgtaaggcc gttgccgttc tggtctatct
      241 ttatcgtgtc accgcttgaa aattcgccag caagggtttt ctctgcaatc gggtcctcaa
      301 tcaggttttg tatcgccctg acaagtggcc ttgcgccaag cttctggtca taggattttt
      361 cgaccagcgc cttgatggca ttgtcagtca gatctacttt caatccctcg tcagtgagtg
      421 tttccgatag cttatttatc agcagacggg caacttggca aagttcctct ttggttagcg
      481 gcttgaatct tataattccg tcaatcctgc ccaaaaattc cgggcggaag accttcttga
      541 tctcagagtc aagcctgtcg ccctctttct catcgtctct gtcttcagta tctgcgccag
      601 caaaaccaag agattttgtg tttttccact gcgaaccgat atttgaggtc atgatgacga
      661 ttgaatgcct gaaatccaca agcctgccct ggctatccgt gagtctgcca tcatccatga
      721 tctgcaaaag aatgttaaac acgtcaggat gggctttttc tatctcatcg aagagcacga
      781 cggagaaggg ttttctcctg attttttctg tgagttgacc gccctcttca aatcccacgt
      841 atcctggagg tgcgccaaca agcctgctca cagcgtgctt ctccatatac tcgctcatgt
      901 ctatcctgag cagggccttt tcagagccaa aaagatggtt tgccaatact ctggcaagct
      961 cggttttgcc aacaccactt gggccaacaa acacaaagga gcctattggt ctgtttcttc
     1021 cggcaagacc aacccttgcc cttctgagtg cttttgatac tgcaacaaca gcctcgtcct
     1081 ggccaatgat cctctcatga agaacttttt ccagggtgag cagtctttcc ctttcctgct
     1141 gaaggagtct cgttgttggt attccggtcc actggctgac aacatcggca atgtcttctg
     1201 ctgtgacaac aaggctcagc ttgtcgacat ctttctgcca ttcctcgcgc ttgatgcgga
     1261 gctggtcttc tatctccctc tctttatcgc gaagctgggc cgccttctca aattcctggg
     1321 actcggcagc cgactgcttt tctttcctga ggctgcgctt tgtggtttcc atttctctga
     1381 tgctttcagg caggttcatc actgacagcc tgacttttgc gcacgcttca tctatcaggt
     1441 cgattgcctt gtctggaagg aaccttccct ggatgtactg ggcagagagc ttggaagcag
     1501 cttccaatgc ggtttgctga atggttacct tgtggtagtt ttcatacctt ggtgcaagcc
     1561 cgatgaggat tttcacggtc tcttcaacag tcggttcttc gacttgtacc atctggaacc
     1621 ttctttcgag ggctgcgtct ttttctatgg attttcggta ttcattcatt gtcgtggctc
     1681 caatgcactg gagctcacct cttgcaagcg ctggttttag aatatttgat gcgtcgattg
     1741 cgccctctgc ggcgcctgca ccaacgattg tgtgtatctc gtctatgaag aggattattt
     1801 tgccctggga gtggtcaatt tcatcaagta tccctttcag cctctcctca aactcgcccc
     1861 tatattttgt tccggcaatg acggcggcaa gatcaagcgc caaaaccctt ttgtctctca
     1921 atgtctctgg gacttcacca tcaacaatgc gctgggcaag tccttctact atggcagtct
     1981 ttccgacacc aggatcgccg acaagcacag gattgttctt tgttctgcgc gagaggatct
     2041 gcgtgattct ctgtatttca accattctgc caactgtcgg gtcaagtttg ccccttctgg
     2101 caagtgcggt caaatccctt gaaaattgct ccacaagaga tttctttcct tttttgtcat
     2161 ggccatgctc ttctacagac tcatcaaccg cagacctgaa acggtccagg tcaatgtcca
     2221 tatcgcgaag gatcctggca gcaagccctt cggcttctga aagaatgcct atgagaaggt
     2281 gttctggtgt gagttttgtg ctgctttgcg ccttcatttc cttctctgca agatcaagcg
     2341 ctctctttgc cctttcagaa agcactatgt ggcttttttg gacagggcca atggccgaat
     2401 tcctgacacc tcttaccaat gacactatgc gatcgcgaag cccctgaaag tcaatttcaa
     2461 gctcccttag agtctgcatt gcaaaacttg tgttttcgcg gatcaggcca agaagaaggt
     2521 gttctgtgcc aagataatca tttccgagct catacgcttc gtcctgtgcc agataaaata
     2581 tccgctgcgt tttttcatcc aaattactcc agttcatgat ttacatggta gcaagcggaa
     2641 aataatttat caacaaaatt tttgtcaata tctggtttgc caatagacaa ccactttcaa
     2701 atggctataa taccagcgaa tgacaaaaga catcgaattt gagggaaacc agaaaaaacg
     2761 gcttttgact tatatttgga ttgctttcgt tttgatagtt atatttggtt ttattttatt
     2821 tttggcacca agttccggcc aaataaaaag ctacattttc agaaccaatg gg
//
LOCUS       DAOSRI010000222         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_32311_length_2818_cov_2.763301, whole genome shotgun sequence.
ACCESSION   DAOSRI010000222 DAOSRI010000000
VERSION     DAOSRI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32311_length_2818_cov_2.763301"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK717_06710"
     CDS             <1..617
                     /locus_tag="PK717_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47385.1"
                     /translation="QALIFSEMRLAIDPKSEELREEVAAIRKQLGLIDSPLVFQEAPL
                     PWLESTKRCLLTLDFQPYFDKCSKSVQEALLSGDIETAKNVRLEWLSVQEFKGVLEER
                     FEGNHSATDDVWAKLMTASTFEQLKPLKDAHPKLLPVLEKILVASRDDVSRLVEIWLD
                     EAQSSVNTVDFEATKVYISLLAQTLPSLAPFLPKIRPILLATVR"
     gene            710..2278
                     /locus_tag="PK717_06715"
     CDS             710..2278
                     /locus_tag="PK717_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOR47386.1"
                     /translation="MNSATFNPYIFYGIAIAFLALLGILAYLGWVRTKNSDDFMLAGK
                     RVPPWLMAISYGSALISTSSIVGFGGKAGEVGLAMVLIVAINIGMGVIMAYIVFGPKI
                     RAVGTKMGAQTMPELLGLRFKSKFVQGVAGVVNFVFMPLYAGAVLVSVSRLMETIIGI
                     NFGIALIIYTVIIAIYVVGGGLKGMIFADAVLGILKFVGVATMFIIVLSKAGGFGAFS
                     AISQVPVPEALAKEGIRSFGTLPTFGSPIWWQLITTLFFGISVGLLSQPQLGVRFLTL
                     KNTRDIKIAVGFGAVFVLVVNGGGILTGALSNLAAYSNPAIGRTAIEMVGMNVDSIIP
                     FAIRSFLPEWLGYIILFTLFSAALSTSTAQIHTMATSLGYDFMIKGLKVKASIGLVKA
                     AALISLAVVIVVGYLLPKSIVATATAMFFGIAASVFLPSICGAIFWKRCTRAASIASM
                     LTGIGTIVFMYFFVHIKESASIGLCNMFFGKPSLFEGHWLSMCDPTCIAVPLSFLALF
                     VVGFLTKVTNEESI"
     gene            2262..2720
                     /locus_tag="PK717_06720"
     CDS             2262..2720
                     /locus_tag="PK717_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47387.1"
                     /translation="MKKAFSIILLCLALASCSSPTSSAIRAYTDYREALKSGEIGNIL
                     AFWHPEMLTRNGDSIKLDIEKKLDTYGILLGYEKMEVLSTKKLENWIFDGIVFKDVIQ
                     LDISIRFPKMDALPPKQSSLVADGPVITRELVYMVDVGGSMRILACETKS"
BASE COUNT          665 a          620 c          696 g          837 t
ORIGIN      
        1 ggcaggccct gatattctct gaaatgaggc tggcaattga cccaaaatcg gaagagttga
       61 gggaagaagt ggctgcaatc agaaaacagc ttggcctgat tgattcacca ctggttttcc
      121 aggaagcgcc gctcccatgg cttgagagta caaagaggtg cttattaaca cttgattttc
      181 agccttattt tgacaaatgt tccaagtcag ttcaggaggc cctgctctcc ggcgatattg
      241 aaacagcaaa aaatgtccgc ctggaatggc tctctgttca ggaattcaag ggcgtacttg
      301 aggagaggtt tgagggcaac cattcggcaa cagatgatgt ttgggccaaa ctaatgactg
      361 cctcgacatt tgaacagcta aaacctctga aggacgcaca cccgaagcta ttgccggttt
      421 tggaaaaaat ccttgttgca tccagagatg atgtttccag gcttgttgaa atctggctag
      481 atgaagcgca atcatcagta aacacggttg attttgaggc cacaaaggtt tatatttcgc
      541 ttctggccca gacgcttcca tcgctagcac cattccttcc aaaaattagg ccaatccttc
      601 ttgcaaccgt gaggtaatac atggatttct ggatcataac ggcaatggtt cttgccattg
      661 cgctgaccct tgttggggta atctatacca ttgtaaattg gggcaaagaa tgaattctgc
      721 tactttcaat ccatatattt tctatggcat agccattgcc tttttggcac tgcttggcat
      781 tcttgcatat cttggatggg tcaggaccaa aaatagtgat gatttcatgc ttgccggcaa
      841 gcgtgttcct ccgtggctga tggccatctc ctatggctcg gctctcattt caacatcatc
      901 gattgttggt tttggtggca aggccggcga agttggtctt gcgatggtcc tgatagttgc
      961 aataaacatc ggtatgggtg tcataatggc ctacatcgtt tttgggccaa aaataagagc
     1021 tgtaggcact aagatgggtg cccagaccat gccggagctc ctcggtttgc gtttcaagtc
     1081 aaaattcgtc cagggtgttg ctggcgtcgt caattttgtt ttcatgccat tgtatgccgg
     1141 tgccgttctg gtgagtgttt cgaggctgat ggaaaccata atcggcatta attttggcat
     1201 tgcgttgatc atatataccg tgatcatagc gatttatgtt gttggcggtg gccttaaggg
     1261 tatgattttt gccgatgctg ttttgggtat cctaaagttt gttggcgttg ccacaatgtt
     1321 tattattgtg ctttcaaagg ctggtggatt cggagctttc tctgcgattt cccaggtacc
     1381 ggtgccggag gcgcttgcaa aggaaggcat cagaagtttc ggaactttgc ctacttttgg
     1441 ttccccaatc tggtggcaat tgataaccac gctatttttt ggcattagtg ttggcctcct
     1501 ttcacaaccc cagcttggtg ttcgtttcct tacccttaaa aacaccagag acatcaagat
     1561 agcagttggt tttggggccg tttttgtgct tgtggtcaat ggcggaggca tcctgacagg
     1621 tgccctgtca aaccttgccg cttacagcaa tcctgcaatc ggcaggaccg caattgaaat
     1681 ggtcgggatg aatgttgact ccataattcc ttttgcaatc agatcgtttc ttccagaatg
     1741 gcttggatac ataatcctgt tcacgctgtt ttctgctgcc ctgtccactt caaccgccca
     1801 gatacacaca atggcaacat cccttgggta tgatttcatg ataaagggat tgaaggtcaa
     1861 agcttcgata gggctggtaa aagcagcggc actcatttca cttgcggtgg ttattgttgt
     1921 tggctaccta ttgccaaaat caattgttgc aacagcaaca gccatgtttt ttggaatagc
     1981 agcctctgtc tttttgccgt ccatctgtgg tgcaatattc tggaaaagat gtactagggc
     2041 agcttctatt gcctcgatgc tgaccggcat cgggacaata gtgttcatgt acttttttgt
     2101 ccatatcaag gaatccgctt caatagggct ttgcaatatg tttttcggaa agcccagcct
     2161 ttttgaaggg cactggctca gcatgtgcga cccgacgtgt attgctgttc cgctgtcttt
     2221 cctggcgctg tttgtggttg gattcctgac aaaggtgacg aatgaagaaa gcatttagca
     2281 ttattttgtt gtgcctggct ctggcctcat gctcttcacc tacttcatct gccatcaggg
     2341 cctataccga ttaccgcgag gctctcaagt ccggggaaat tggaaacata cttgctttct
     2401 ggcacccgga aatgctcaca agaaatggtg actcaatcaa gctggacatt gagaaaaagc
     2461 tcgacacgta tggcatactc cttggttacg aaaagatgga agtgctctca acgaaaaagc
     2521 ttgaaaactg gatattcgac gggattgtat tcaaagacgt catccagctt gatatttcta
     2581 taagattccc gaaaatggat gcccttccac caaagcagag ttcccttgtg gccgatgggc
     2641 ctgtgatcac cagagagctt gtttatatgg tcgacgttgg cggttcaatg aggattctgg
     2701 catgcgaaac aaaatcctga cagtattaat cttttcattg tttcttgcct catgcggacg
     2761 gtcaccggtt tttgagggtg attttaaggg tgggcagggt gaagcttgcc atgccgtc
//
LOCUS       DAOSRI010000223         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_32418_length_2811_cov_4.191945, whole genome shotgun sequence.
ACCESSION   DAOSRI010000223 DAOSRI010000000
VERSION     DAOSRI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32418_length_2811_cov_4.191945"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK717_06725"
     CDS             <1..589
                     /locus_tag="PK717_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOR47388.1"
                     /translation="VGRGEEISDLDLTPKARAEAMEKLARMQDPDIEIMSETPHYATE
                     TSMIGGDCQQKLPASNCFPITAFFDMGPSRKFFRAFKDILGGCPAGRLYCNIQPNGDL
                     TPCMFSPFDPVVGNLTRQSFKEAWKGFNVMWDRTKLTGFCGECAHSIDCGGCRARAVA
                     KGNLMGPDYGCFTARYFSEARRGHRKDKAGSVKSR"
     gene            507..1571
                     /gene="pheS"
                     /locus_tag="PK717_06730"
     CDS             507..1571
                     /gene="pheS"
                     /locus_tag="PK717_06730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOR47389.1"
                     /translation="MVASRPDTSRRQGVDIEKIKQEALNLVEQSKSSKELEDIRVRFL
                     GRKGELTSLLRELGNLSPEERKSAGANLNSAKEEIEAKIESRLSDISRKEMQEKLFAE
                     KIDITMPGRPVMKGAMHPLSIVAEDITEVFHKLGFVVRQGPIIESDWYNFEALNIPQW
                     HPARAMQDTFYIEGNRLLRTHTSPVQIRTMESEKPPIRMIAMGLCHRRDATDASHSPF
                     FHQVEGLMIDRHISFSDLAGILSIILSNVFGGNVKVKFLPSFFPFVEPGAETLASCPF
                     CDGKGCRVCQNSGWIEMGGSGMVNPKVLKNVGYDPEEYQGFAFGWGIERIAMLKFGIS
                     DIRDFTTNDLRFLSQFGGVR"
     gene            1568..>2811
                     /locus_tag="PK717_06735"
     CDS             1568..>2811
                     /locus_tag="PK717_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOR47390.1"
                     /translation="MKLSYRYLKSVVPWDIDLETIQEGFKILGLEVEDLQKRDRVRGS
                     VFGKITEIRQATEKLHIAKIEIAPGKTIEVVINSDRVKPGQIYPVAIHGTRIGDVTVE
                     PRNIRGFASEGAILSAQDFGVDPEILPQAEREGPWQMPDDTPLFTDPAESFWLSDSIL
                     DIKITPNHPEWLSLRGLLHEVAAILWRKTGKIHEVPSLETSKNISAENTCLEFRVDIE
                     DYEDCQRYIGMLANVSVAPSGFETRRKLLASGTRPINNVVDASNLSMYEDGQPTHAFD
                     ADKIKSGVVRVARTKAPMKFKTLDDKERDLPVGTLMIWDGEKPVGIAGIMGGQDSEVT
                     SATKRIFLESANFNPLLIAKSSSILGIRSEASTRFEKGADVEVAEDTAWKVISMIGTS
                     GCAPVESYNKKERRQVQVRVKR"
BASE COUNT          814 a          657 c          743 g          597 t
ORIGIN      
        1 cgttggaagg ggtgaagaga tatcagacct cgatcttacg ccaaaagccc gcgccgaggc
       61 catggaaaaa ctggccagaa tgcaggaccc ggacatcgag ataatgtctg aaacacccca
      121 ctatgcaact gagacatcca tgattggcgg ggactgccag caaaaacttc ctgcctcaaa
      181 ctgtttcccg ataacggcat tctttgacat ggggccttcc agaaaattct tcagggcgtt
      241 caaggacatc cttgggggct gccctgcggg aaggctctac tgcaacatac agccaaacgg
      301 cgacttgacc ccatgcatgt tctcgccatt tgatccggtg gttggaaatc tcacaagaca
      361 gagcttcaag gaggcctgga agggctttaa tgtcatgtgg gacaggacaa agctgaccgg
      421 tttttgtggc gaatgcgcac attccataga ttgcggggga tgcagagcaa gggcagttgc
      481 aaaaggaaac ttgatggggc cagattatgg ttgcttcacg gcccgatact tctcggaggc
      541 aaggcgtgga catagaaaag ataaagcagg aagcgttaaa tctcgttgag cagtcaaaaa
      601 gctccaaaga gcttgaagat ataagggtaa ggttccttgg cagaaagggc gagctgacct
      661 cactcctcag ggagcttggc aacctctctc cagaagaaag aaaatctgcc ggcgcaaacc
      721 ttaacagcgc caaggaagag atcgaagcaa aaattgaatc cagactctcc gacatatcaa
      781 gaaaagagat gcaggagaag ctttttgcag aaaagataga cattacaatg ccaggcaggc
      841 ccgtaatgaa gggcgccatg caccccctgt ccattgttgc cgaagacata accgaagtgt
      901 tccacaaact tggttttgtc gtgaggcagg ggccaataat cgaatctgac tggtacaatt
      961 ttgaggccct gaacattccg caatggcacc cggcaagggc catgcaggac acattctata
     1021 ttgaaggcaa caggctcctg aggacccaca cctcacctgt ccagataagg acaatggaga
     1081 gcgaaaaacc gccaataagg atgattgcca tggggctctg tcatcgcagg gacgcaacgg
     1141 atgcctccca ctcgccattt tttcatcagg tcgagggtct gatgattgac cgtcacatct
     1201 ctttttctga tctcgcaggg atattgtcta tcatcctttc aaacgttttt ggtggcaacg
     1261 taaaggtaaa attccttcca tcgtttttcc cgttcgtcga acctggcgca gagactctgg
     1321 cttcgtgccc attctgtgat ggaaaggggt gcagggtctg ccagaacagt ggctggattg
     1381 aaatgggcgg atccggaatg gtgaacccga aggtactgaa aaatgttggc tacgatccgg
     1441 aagaatatca gggcttcgct tttggctggg gaatcgagag gatagcaatg ctgaaatttg
     1501 gcatcagcga cataagggac ttcaccacaa atgacctcag gttcctttcc cagttcggag
     1561 gtgtcagatg aaactctcgt atcgctatct gaaaagtgtt gttccgtggg acatcgacct
     1621 ggaaaccatc caggaaggtt tcaaaatatt gggccttgaa gtcgaggacc ttcaaaaaag
     1681 agacagggtc aggggctcgg tttttggaaa aataactgaa ataaggcagg caacagaaaa
     1741 actgcacata gcaaaaattg agattgctcc aggaaaaacc atcgaggttg tgatcaactc
     1801 tgatagggtg aaaccaggcc agatttatcc ggtcgcaatc catggaacgc gcattggcga
     1861 tgttacagtc gagccaagaa acataagggg attcgcttca gaaggggcaa tcctttccgc
     1921 ccaggatttt ggcgttgatc cagaaatcct tccacaggca gagagggaag gcccgtggca
     1981 gatgcccgat gatactccgc tgtttacaga cccggcagaa tcattctggc tctcagactc
     2041 gatactggac atcaagataa cgccaaatca tccggaatgg ctgtcgctcc gcgggctcct
     2101 tcacgaggtt gccgcaatcc tttggaggaa aaccggcaag atacatgaag tcccatcgct
     2161 tgagacatca aaaaatatct ctgctgaaaa cacctgcttg gaatttagag ttgacataga
     2221 agattatgag gattgccaga gatacatcgg aatgcttgca aatgtaagcg ttgcgccctc
     2281 aggatttgag accagaagaa aattactggc ttcaggcaca aggccaataa acaatgttgt
     2341 cgatgcctcc aatctttcca tgtatgagga cggacagcca acccacgcct ttgatgcaga
     2401 caaaataaag agtggcgtgg taagggtcgc aagaacaaaa gcaccaatga aattcaaaac
     2461 ccttgatgac aaggaacgcg accttccggt tggcaccctt atgatctggg acggcgagaa
     2521 accggttggg atagccggca tcatgggtgg acaggactcg gaagtcactt ctgcaacaaa
     2581 aaggattttc ctcgaatctg cgaacttcaa ccctcttctt atagccaaat ccagctcgat
     2641 ccttggaata aggagcgagg cttcaacaag gttcgagaag ggcgcagatg tggaggtcgc
     2701 cgaggacacc gcctggaaag tcatctcgat gattggaaca agcggatgtg ccccagtgga
     2761 aagctacaac aaaaaagaaa ggcgccaggt ccaggtaagg gtcaagagaa t
//
LOCUS       DAOSRI010000224         2745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_33443_length_2745_cov_3.879926, whole genome shotgun sequence.
ACCESSION   DAOSRI010000224 DAOSRI010000000
VERSION     DAOSRI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33443_length_2745_cov_3.879926"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..305
                     /locus_tag="PK717_06740"
     tRNA            214..305
                     /locus_tag="PK717_06740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Ser,seq:gga)
     gene            404..760
                     /locus_tag="PK717_06745"
     CDS             404..760
                     /locus_tag="PK717_06745"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOR47391.1"
                     /translation="MGKTVEEYIESQKSPQKEILRRLREIFLKTLETPEENVGWGVVI
                     FGRGKFYIVALKDRVHVGFAITGLDKDEIRLFEGSGKTMKHIKVYSVDDIDEEKIVGL
                     IKMVDKKTVFTENIGN"
     gene            976..1467
                     /locus_tag="PK717_06750"
     CDS             976..1467
                     /locus_tag="PK717_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47392.1"
                     /translation="MNDNNKNIEETGCCPRFDPTPWDNKEFDWKDKKFIKDKVFTMFY
                     APVNFGPVIRRITKKVEDAKGQMPDWLCLSDHTSKWNMDLYLAVDKEIPGAENITLSG
                     KYLSRVYEGDFKDTGKWCKDFEDYVKGKGQEVKKWYMWYTTCPKCAKVYGKNYVVIVG
                     KIA"
     gene            complement(1640..>2745)
                     /locus_tag="PK717_06755"
     CDS             complement(1640..>2745)
                     /locus_tag="PK717_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOR47393.1"
                     /translation="HKKFYLWDYENDALKEIFANIDSEIRVVDVSRDGKKLLIQTSSQ
                     ARDSHIVYKLEDGSFLSLDSLDGVSSYSYILEDEVLLLNSSSQHKSRILNYDLNTGEF
                     KRVLLELSGSIPASGARDVRFIGARDDKVQGWLRIPVGNGPFPTIIELPGGPSCAYSC
                     YESDVFLDHGFAKLTINYHGSSSFGYDFQKSIIGRVGELELEDMVAARSWLTEQGISN
                     PNQIFLMGGSYGGYLTLWGLVRRPDLWKAGLARVPIADFVAAYEDENGSLKKSDNYLF
                     GGSLYDKPELYRQSSPITYIEHLNAPLQIIAGKHDTRCPRRQIELFIEKGKELGKDIE
                     TYWYDEGHSSNSINEQIHQIELRLKFLKKVMDG"
BASE COUNT          867 a          519 c          582 g          777 t
ORIGIN      
        1 cattctccat ggtggaaacg atttatccct ggataaagtt tgaagtaagt ggggtaaatg
       61 tcagagaggt cctggaagaa gcaatcctgg atgaaaactt tgtgggcgag gttgtaaaca
      121 tcgaaggtga ctttggggag acttgcacag tcaggatagt ataagatttg acttgacacc
      181 gacagggcaa atccggataa ttttgccctg tcaggagagt tggctgagcg gtcgaaagca
      241 cccgcctgga gagcgggtaa ctgggtcaaa ctggttcggg ggttcgaatc cctcactctc
      301 cgccagtttc agtatgggga tccaaaaata aacatcagat tattttttcg catcaacaca
      361 atgtttttaa tcgcacatat ttcaaggagg tcatcacaac ttcatgggga aaactgtaga
      421 agaatacatc gaaagtcaaa aatcaccaca aaaggagata ttaaggaggc tcagggagat
      481 attcctgaaa actcttgaga caccagagga gaatgttggt tggggtgttg ttatttttgg
      541 tagagggaaa ttttatattg ttgcattgaa agatcgggtt catgttggtt ttgccataac
      601 cggtctggac aaagacgaga taagattgtt tgaaggaagt ggcaaaacca tgaaacatat
      661 aaaagtttat tcggtggatg atattgatga agagaaaata gtagggctga tcaaaatggt
      721 ggataaaaaa actgttttta cagaaaacat tggaaactga ttctaatgtt acttttttga
      781 cagctcgcca gaaataattc agtgaatcca atagattatc aagacaaaaa tttgagaaat
      841 acaacaacct caaaaagttg attttgaaaa aacggatcgg gaacaacttg acaatgtccg
      901 gtgctttgtt actttacagc catgagcccg acagggctta atcaatttgc aaaagaaagg
      961 ggcttagtaa aggccatgaa tgataacaat aaaaatattg aagaaaccgg gtgttgccca
     1021 agatttgatc ccacaccatg ggacaacaag gaattcgact ggaaggataa aaagtttata
     1081 aaagacaagg tgtttacgat gttttatgcg cctgtcaatt ttggtccggt gatacgccgg
     1141 ataaccaaaa aggtagaaga tgccaaagga cagatgcctg actggttgtg cctttccgac
     1201 catacttcaa aatggaacat ggacctgtat ctggctgtgg acaaggaaat ccctggggct
     1261 gaaaacatta ccctgagcgg aaaatatctg agcagggtct acgagggaga tttcaaagac
     1321 actggaaaat ggtgcaagga ttttgaggat tacgtcaaag gcaaagggca ggaagtcaaa
     1381 aaatggtata tgtggtacac aacttgcccc aaatgcgcca aggtttatgg caaaaattat
     1441 gtggttattg ttgggaaaat agcataataa agacaattat cactgaaaaa attatgaaaa
     1501 tttgaaggtt ggtgtgattc cagaaacaac accgaatgca atatacggag ttgaaggaaa
     1561 ctggattttt ctaatgcata cttttttatg gtttcatgga aataatcatg gctttgtttc
     1621 caatcttcac aaataattct tacccatcca taaccttttt gaggaatttc agtctaagct
     1681 caatctggtg tatctgttca tttatcgaat tgcttgagtg tccttcgtca taccagtatg
     1741 tttcgatgtc cttaccaagt tcctttccct tttcaataaa tagctctatt tgccgccttg
     1801 gacatcttgt gtcatgtttg ccagcaatta tctgcaatgg cgcatttaaa tgctcgatat
     1861 atgttattgg tgaactctgt ctgtatagct ctggcttatc ataaagagaa ccaccaaata
     1921 agtaattatc cgatttcttg agagatccat tttcatcttc ataggcagca acgaaatcag
     1981 caatcggcac acgagcaagt cctgctttcc acaaatcagg ccttcttact aaaccccata
     2041 acgtcagata cccgccatat gaacccccca taaggaatat ctggtttggg ttggaaatac
     2101 cttgttcggt tagccatgat ctggcagcca ccatgtcttc tagttcaagt tctcccaccc
     2161 tgccgatgat cgatttttga aaatcataac caaaagatga tgagccatga tagttaattg
     2221 tcaattttgc aaaaccatgg tcaagaaaca cgtcggattc ataacaacta tatgcacagc
     2281 taggacctcc tggtagctcg ataattgttg gaaatggtcc atttccaacg gggattctaa
     2341 gccatccctg aactttgtca tctctggcac ctataaacct tacatctcta gcaccgctcg
     2401 caggtatcga tcctgaaagc tccagcaaaa cccttttgaa ttctccagta ttcaaatcat
     2461 aattcaatat gcgtgattta tgctgtgagg atgaatttaa taatagcacc tcgtcttcca
     2521 atatgtagga atagcttgaa acaccatcga ggctatcgag tgaaagaaaa gaaccatcct
     2581 ctagtttgta tacaatatgg ctatctctag cttgtgaact tgtctgaatg aggagtttct
     2641 ttccatccct ggagacatcc acaactcgaa tctctgagtc tatatttgca aatatttcct
     2701 ttagtgcatc attttcataa tcccataaat aaaatttctt atgat
//
LOCUS       DAOSRI010000225         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_33886_length_2720_cov_3.407129, whole genome shotgun sequence.
ACCESSION   DAOSRI010000225 DAOSRI010000000
VERSION     DAOSRI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33886_length_2720_cov_3.407129"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK717_06760"
     CDS             <1..362
                     /locus_tag="PK717_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HOR47394.1"
                     /translation="KSLIEPKIQNMQDRLQGMIAITNCLGWGLVTDYKLDESAKTLQL
                     TIKHSYYIDAYKNRYGKNSEYPICYMWTGTAGGYMDLLFGQKPNTFVATETTCAAKSG
                     DICVFEAKISKTKFGFN"
     gene            410..763
                     /locus_tag="PK717_06765"
     CDS             410..763
                     /locus_tag="PK717_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOR47395.1"
                     /translation="MLSRVQNSIPGMKSAMVASSDGLLLASTSFSEENERTAAMSASL
                     VSISRRASEAMGKPRLNDVTIRSEEGYITLFSVGRDAVLVISTTSEQKNLGMVYLEGK
                     SASNELTEILKRSGL"
     gene            726..1934
                     /locus_tag="PK717_06770"
     CDS             726..1934
                     /locus_tag="PK717_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR47396.1"
                     /translation="MSSQKSSREVVFKNQFPSASEYHNLDAAATGLLPESVAAFVYDY
                     HLKSCVNAGRSAYRRSIEVTAMVDEAREKIASFLGTKKTNLAFAPGATHAINWIAKGL
                     DLSAGDTVLCTMADHHANILPWLDLKRRGIRVVFAMCDGLGRIDVADFQDKVKGAKVA
                     AFIAASNVTGAIQPMRELTKISSENGAISVVDAAQLAPHQRIDFDSLGVDFMAIAGHK
                     MMASKGSGLLLARESAQAFIGPLIVGGGSIKDVKTDSFELLPFPEGFESGTPSIEGIL
                     SLWKAIEWLQERGEENVWRHEKTLNKQAREILSQVRGLRIYQPENCTPTMSIELSGWE
                     PNKLAVKLDREYNIAIRSGHMCALPLVRDTMGFPKGLCRISFGPWNEEKDIVAIANAL
                     DKISHEKTNW"
     gene            1941..2207
                     /locus_tag="PK717_06775"
     CDS             1941..2207
                     /locus_tag="PK717_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47397.1"
                     /translation="MNIKVIELCGYGPTADYEAVDLDNPKLDMSYIPGRWEFTGGNDE
                     ARKFTMEIMELQVYNEGKLILFGAPSGTCGKDMARKLLEEWPRI"
     gene            2289..>2720
                     /locus_tag="PK717_06780"
     CDS             2289..>2720
                     /locus_tag="PK717_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47398.1"
                     /translation="MSKTLRMALAIVLPFIVLFPSFATSQSSKIVQIGKNILSKPHSI
                     CVLNGQLFITDTQLNEVLCFSTSGELVARKNVFGAIKLSSPMGICSLPTGEIVVADSG
                     NKRILLLDQFMSELRSFNVEDKYFEKPVDVAELGGAILVLDN"
BASE COUNT          786 a          545 c          656 g          733 t
ORIGIN      
        1 ggaagagcct tatagagcca aagatacaga acatgcagga cagactccag ggaatgatcg
       61 ccataacgaa ctgccttggc tggggtcttg ttacagacta caaacttgat gaaagcgcaa
      121 aaacactcca gctaactatc aagcacagtt actatattga tgcctacaaa aacagatatg
      181 gcaagaattc tgagtatccg atttgctata tgtggaccgg cacagctgga ggctatatgg
      241 acttgctatt tggacaaaag ccaaatacat ttgtggccac agaaacaact tgcgcggcta
      301 agtcgggcga catttgtgtt tttgaggcaa aaatatccaa aacaaaattt ggattcaact
      361 aggaaagagg tgaaagaaaa tgccaacagt aggggaacaa atgactcaag tgctcagtag
      421 agtacaaaac tcgattcctg ggatgaagag cgccatggta gcgtcttctg acggccttct
      481 cctagcctcg acttcattct ccgaggagaa tgagagaact gctgctatga gtgcatcact
      541 agtttcaatt tccagaaggg catcagaggc gatgggcaaa ccgcgtctta atgatgttac
      601 gatccgctct gaagagggct acataaccct gttctcagtt ggaagagatg ccgttttggt
      661 tatctccact acatctgaac agaaaaatct tggaatggtc taccttgaag gcaagagcgc
      721 atcaaatgag ctcacagaaa tcctcaagag aagtggtctt taaaaaccaa tttccaagcg
      781 catcagagta tcataaccta gacgccgccg cgaccggtct tttgcctgag tctgtggcgg
      841 cgtttgttta tgattaccac ctcaagtcgt gtgtcaatgc tggcagatca gcttacaggc
      901 gatcaattga ggttacagcg atggttgatg aagccaggga aaaaatcgcc tctttccttg
      961 gaacaaagaa aactaatctg gcttttgcac ctggtgctac ccatgcaatt aattggattg
     1021 cgaaaggtct ggatctttca gcaggcgata ctgttttgtg cacaatggcc gaccatcatg
     1081 caaatattct tccgtggctt gatcttaaaa gaagaggcat cagggttgtt tttgcaatgt
     1141 gcgatggtct cggaaggatc gatgttgccg attttcagga taaggtcaaa ggagcaaagg
     1201 ttgccgcttt tatcgcagca tcaaatgtta ctggcgccat tcaacccatg agggaactga
     1261 caaagatttc ctctgaaaat ggtgcaatct ctgttgttga tgccgcccag ctggcaccac
     1321 accagagaat tgattttgat tccctgggtg tggattttat ggcaattgcc ggtcacaaga
     1381 tgatggcttc aaaaggctcc ggattgcttt tggcaaggga gagtgcccag gcctttattg
     1441 ggccgctgat tgttggcggt ggttcaataa aagatgttaa aacggattcc tttgagctcc
     1501 tcccgtttcc ggaagggttt gagtcgggga caccttcaat agagggcata ctcagcttgt
     1561 ggaaagccat tgagtggctc caggagcgag gcgaggagaa cgtctggcga catgaaaaaa
     1621 ctcttaacaa acaggcaaga gaaattcttt cacaagttcg tggtttaagg atttaccagc
     1681 cagaaaattg cacgccgacg atgtccattg agttgagtgg ttgggagcca aacaaactgg
     1741 ctgtgaagct tgatagggaa tacaacatcg ccatccgttc cggtcacatg tgcgcgcttc
     1801 ctcttgtgag ggacacaatg ggtttcccga aaggcctttg tagaatttcg ttcgggccat
     1861 ggaatgagga gaaagatatt gtggcaattg caaatgctct agacaaaatt tctcatgaga
     1921 agacaaactg gtgactttta gtgaacataa aggtgatcga actgtgcggc tatggcccaa
     1981 cagctgatta cgaggctgtt gatttagata acccaaagct tgatatgtct tatatccctg
     2041 gaagatggga gtttacagga ggaaatgacg aggccagaaa atttaccatg gaaatcatgg
     2101 aattgcaggt ctataacgaa ggcaaattga tcctttttgg tgcaccaagc gggacctgtg
     2161 gaaaagatat ggcgcgaaaa ctccttgaag aatggccaag aatttgacca aaaaattaca
     2221 atttcaaatt attttttgtc tggtattcgg ttgacctccc cctgtattat tgatatatct
     2281 tacccaagat gagcaaaaca cttaggatgg cactcgcgat tgttttgcca tttattgttc
     2341 tttttccgtc ttttgctacc tcacaaagta gtaagattgt ccagattgga aaaaatattc
     2401 tttcaaaacc tcattcaatc tgtgttttaa atggtcagct ttttataaca gatacccaac
     2461 tcaatgaagt cttgtgtttt tcaacatcag gtgaactcgt tgccagaaaa aatgtttttg
     2521 gagcgataaa actttcatca ccaatgggaa tttgctcgct tccaacagga gaaattgttg
     2581 ttgctgattc aggtaacaaa cggattttgc ttctggacca gtttatgagt gagttacgtt
     2641 catttaatgt tgaagacaaa tattttgaaa aaccggtgga tgttgctgaa ttgggcggag
     2701 ccatacttgt tcttgataac
//
LOCUS       DAOSRI010000226         2708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34106_length_2708_cov_3.074256, whole genome shotgun sequence.
ACCESSION   DAOSRI010000226 DAOSRI010000000
VERSION     DAOSRI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34106_length_2708_cov_3.074256"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2708)
                     /locus_tag="PK717_06785"
     CDS             complement(<1..>2708)
                     /locus_tag="PK717_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47399.1"
                     /translation="PWGGEGLIGFSSAYRCTDIGTGAFGCQRSIYRKYRSNTNQVESA
                     DIRITEVTITKGNNTVTYKAGTNVTAGDLDCGAPLLNLPTTLRFYDVIHGENQPNGEY
                     DVGEDIYNDLSNNLIIDYGDVRMSQIKIGYYSYPCSDVVDEWGCFVHEYKAHGFSIGK
                     CGHPTAIDIPVLPGKVGLDLYVDRELRVEQTSKLRIKSPILLEKNQKIYVTLRSPATY
                     GKIPWEQTSTITDADREIYFEITPYQGSLYSNGRYDPLVVTAFADFGDPVKVAPPDGP
                     YQDMFYLTKYMPTDNVQNNKNTLTAVPPPWPQPAVPISLANKYDAWEKGYLKVRSERL
                     EINSTRKCLDMLEERYPNISLETYDADNMLDINDPCCIPFALNKDEQTIVSYNATGGG
                     VNWMATAIGENGQRYIIQYNIDKTYYFWYWNDIGYVPGALDSGDYVGDDPSFMPPLFV
                     GTSRPIEVSSQASLVDVNNSSQMAQTWGDDDGFLAWGVVTKGDELGLFDGITTDPKTG
                     VKFGRIETYGVPTYITSASTFTDTDTGGWALGLVKPKENTQIRLRISSFNIMFDYNSK
                     LPVHPSHFLLDDHLGLDYSGYATAKVLTLDPVLNFSELEVIDHGLQFSQLDYTAGKNS
                     INQISPPTPQIQHPYNPMLRNLQDDFRVYPGGQAHLSRIINIPFARAMICGYNSYPAI
                     WSEWGQRLNANKEALGKDPDEDKKQRKVNFNKLGTEFFPLTDYGIYFVLKDSNGTHLT
                     FNPGTTSNPTPFKRLIKKLTVTGPFKRPKIMDPITGRVTTDFAFEGMVRLPIIYDFTG
                     KVVIDSTNYQWYEFEGQNWIGEIGFGPDKITFMPDEFNKFLIWNKRLDYTGVSNVIKI
                     DELTPVGSGRIEIEVELFDGTIKKFQDCCGNKNEFGIRVHGLTITG"
BASE COUNT          651 a          590 c          603 g          864 t
ORIGIN      
        1 ggcccgtgat tgtgagccca tgcaccctta ttccaaattc gtttttgttg ccacagcagt
       61 cctggaattt ctttatggta ccgtcaaaca actcaacttc gatttctatc ctgcctgaac
      121 caacaggggt caattcatca atctttatga cgtttgaaac gcctgtataa tccagccttt
      181 tgttccatat gaggaatttg ttgaattcat caggcatgaa tgttatcttg tctgggccaa
      241 aacctatttc accgatccag ttttgccctt caaactcgta ccattgatag ttagtactgt
      301 ctatgactac ttttccagtg aaatcatata tgattggaag tctgaccatt ccctcaaagg
      361 caaagtcagt tgtgaccctt ccagttatcg ggtccatgat ctttggcctc ttgaatggcc
      421 ctgtaaccgt gagtttcttt attagtcttt tgaatggtgt tggatttgat gtggttccag
      481 gattgaatgt caaatgagta ccatttgagt ctttcaagac gaaataaatg ccgtaatcag
      541 tgagcgggaa gaattcggta ccaagcttgt taaagtttac cttcctttgt tttttgtctt
      601 catcggggtc tttgccaagt gcttctttgt ttgcattgag cctttgtccc cattcggacc
      661 atattgcggg atatgagttg tagccacata tcattgctct ggcaaaagga atatttatga
      721 tcctgctcaa gtgcgcctgt ccgcctggat agaccctgaa atcatcttgc aaattcctta
      781 gcattggatt gtatgggtgc tggatttgtg gagttggcgg cgagatctgg tttatggagt
      841 ttttccctgc tgtgtaatcc agttgcgaga attgcagacc atgatcgatg acctcgagtt
      901 ctgagaagtt gagtactggg tcgagtgtaa gaacctttgc tgttgcataa ccgctgtaat
      961 caagaccaag gtgatcatca agcaggaagt gtgaagggtg gactggtagt ttgctgttat
     1021 agtcgaacat aatgttgaaa ctgcttattc tcagccttat ctgggtgttc tcttttggtt
     1081 ttacaagacc cagtgcccac ccaccggtat ctgtatctgt gaatgttgaa gcactggtga
     1141 tatatgttgg gacaccataa gtctcaattc ttccaaattt aacacctgtt tttgggtctg
     1201 tggtaatgcc atcgaacaat ccgagttcat caccctttgt gactactccc caggcgagga
     1261 aaccatcgtc atcaccccat gtttgggcca tctgcgagga attgtttaca tcaaccagcg
     1321 atgcctgtga tgaaacttct attggtctgg atgtcccaac aaatagaggt ggcataaagc
     1381 ttggatcatc accaacatag tcacccgaat caagtgcccc aggaacataa cctatatcat
     1441 tccaatacca gaagtagtat gttttgtcta tgttgtattg gattatgtat cgctgtccgt
     1501 tttcacctat ggcggttgcc atccaattta cgccaccgcc agtggcatta taggaaacaa
     1561 ttgtttgctc gtccttattg agggcaaacg ggatgcaaca tgggtcgtta atatcgagca
     1621 tgttgtctgc atcataggtc tcaagagaga tgtttggata tctctcttcg agcatgtcca
     1681 gacactttct tgtggaattt atttcgagcc tttcactcct gactttcaga tagccctttt
     1741 cccatgcgtc atacttgttt gcaagtgaaa tcggaacagc cggttgcggc catggaggag
     1801 ggactgcagt gagagtattt ttgttgttct gaacgttgtc tgtgggcatg tattttgtca
     1861 ggtagaacat gtcctgataa ggcccatctg gtggcgctac ttttactggg tcgccaaaat
     1921 ctgcaaaagc cgttacaacg agcggatcgt atctcccatt tgaataaagt gaaccctggt
     1981 aaggggttat ttcaaaatag atttctctgt cggcatcagt tattgtgctt gtctgttccc
     2041 acggaatttt tccataagtt gctggtgacc tgagggtaac atagattttt tggttttttt
     2101 ctagcaaaat tggacttttg atacgcaact ttgatgtctg ttcaacacgc agttccctgt
     2161 caacataaag atcaagacca acttttccag ggagtactgg aatatctatt gctgttgggt
     2221 ggccgcattt accgattgag aaaccatgag ccttgtattc atggacaaaa caaccccatt
     2281 catcaacaac atcactgcac ggatatgagt agtagccgat ttttatctgg ctcattctca
     2341 catcaccata gtcgatgatc aggttgttac tgaggtcatt gtatatatct tcgccaacat
     2401 catattcgcc atttggctga ttctcaccat ggatcacatc gtaaaaacgt aatgtggttg
     2461 gcaaattaag gagtggggcg ccacagtcca ggtcgcctgc ggttacattt gtgccggcct
     2521 tgtaggtgac agtattgttg ccctttgtga tcgtgacctc tgttatcctt atgtcggctg
     2581 attctacctg gtttgtgttg cttctgtatt tcctgtaaat cgatctctgg caaccaaatg
     2641 cacccgttcc aatatcggtg catctatacg cagaagaaaa acctattaga ccttcgccgc
     2701 cccaaggg
//
LOCUS       DAOSRI010000227         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34224_length_2700_cov_4.272968, whole genome shotgun sequence.
ACCESSION   DAOSRI010000227 DAOSRI010000000
VERSION     DAOSRI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34224_length_2700_cov_4.272968"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK717_06790"
     CDS             <1..778
                     /locus_tag="PK717_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47400.1"
                     /translation="MISVDAIFSDYEGQNVLQIDATDFGRFVFVGSKEGLDYSYDATE
                     HARHLAGEKYLLVLKTIKTKPSRITLAPLNQNLKRIYDMLFRCKPKFVGLSTNYVSFW
                     RLAETFKEDGNTCIVSIDMENLTMWAIFNAGIMTGGWLCKDGVYPSELLTNQKILESS
                     VKMVGTINVYESRQTSVFEVTTKTKLKRARDFADIFNSESLISQRAKWLHGESVADIA
                     MLVDGARTISDIALAINEPVDKVLNTLRFLAEQGLVERFQ"
     gene            775..1278
                     /locus_tag="PK717_06795"
     CDS             775..1278
                     /locus_tag="PK717_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47401.1"
                     /translation="MKNMLPKLAGLPGKIGLCSQLNISLPTKGIHEMLGTRRAKPVLY
                     QLGRSIGRELGAILEKEHGFKKANGTDALFANIEKMGHLFFGCRISTPKNSGPIATLR
                     IFDSPGCAGISQMPEPCCDYIAGVIASFSAYQLGGVPTSCIEISCRGCDPTLPCCEFE
                     LLIGTLK"
     gene            complement(1270..>2700)
                     /locus_tag="PK717_06800"
     CDS             complement(1270..>2700)
                     /locus_tag="PK717_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47402.1"
                     /translation="ILGETKGWPIEAYDAGLAFDASFMYYGAAQMSLAVDSTIALLAK
                     EKPSPFEVDTPLGRITISGDGDDRHDCQKQLLLLDTGGNDTYTGWVAGNNGLENPVSV
                     SIDLSGNDTYKTDYNFHNCMGSGRFGFACLYDAEGDDNYNAIECSQGAASFGVGVLVD
                     KKGDDHYNSINLSQGAAEFGLGILQDQEGTDVYESYFASQGFGFTQGIGLLQDKSGND
                     TYIANDTDIINPSAQSKEHNTSMCQGAGYGLRAEFDNGHSMSGGVGILQDMGGDDSYS
                     CGVFGQGVAYWHGMGILSDYTGNDNYKGTWYVQGSCAHFGLAYFQERAGNDTYQATMA
                     TSIGVGHDTSVGINVDLGGNDTYNCFRMEGGKKVYAGLVAGCGNNNGIGILANIGGDD
                     TYDIGATPSLGQYGGPMPCNGGTTRDTMINIGIFLDIGGNDTYTAKHAKENAKWIQEP
                     PKDCPDRKTDFGIGVDIKDGAIKPLL"
BASE COUNT          675 a          652 c          627 g          746 t
ORIGIN      
        1 catgatcagt gttgacgcta tcttctctga ttatgaaggg caaaatgtcc ttcaaattga
       61 tgctaccgat tttggaaggt tcgtttttgt tggatcaaaa gaaggtctcg attactccta
      121 tgacgccacc gaacatgcca gacacctggc tggcgaaaaa taccttcttg ttcttaaaac
      181 aattaaaaca aagccatcac gaataactct tgcacctctt aaccaaaatt tgaagcgtat
      241 atacgacatg ctcttcagat gcaaacctaa atttgtcgga ttgtccacaa attatgtttc
      301 gttctggaga cttgcagaaa catttaaaga agatggaaac acctgcattg tttcgattga
      361 catggaaaat ttaacaatgt gggcgatctt caatgctggc attatgaccg gaggatggct
      421 ttgcaaagat ggtgtctacc cgtccgaatt gcttacaaat cagaaaatcc tggaatcatc
      481 ggtaaaaatg gttggcacca taaatgtata tgaaagcaga caaacatcag tttttgaagt
      541 tacgacgaaa accaaactaa agagagcgcg ggattttgct gatatattca attctgaatc
      601 gctcatcagc cagcgggcca agtggctgca tggtgaatct gttgctgata ttgctatgct
      661 tgttgatggg gccaggacaa tctccgatat agcattggca atcaatgaac ctgtcgacaa
      721 ggttttaaat accctcaggt tcctggcgga gcagggcctt gtggagagat tccagtgaaa
      781 aacatgttgc caaagctggc tggtttgcct ggcaaaattg ggctttgttc acaattgaac
      841 atatcgctcc caacaaaagg catacatgaa atgttgggca caagaagggc aaagccagtg
      901 ctttaccagc ttggaagaag cattggcagg gagttgggtg caattcttga aaaagaacac
      961 ggcttcaaga aagccaacgg aacagacgct ttgtttgcaa atattgaaaa gatgggccat
     1021 ctgttttttg gctgcagaat atcgacgccc aaaaactcag gacctattgc caccctcaga
     1081 atatttgatt caccagggtg cgctggcatc tcgcagatgc cggagccctg ttgtgactat
     1141 attgctggtg ttatcgcatc cttttcggcg taccagcttg gtggtgtgcc gacttcatgc
     1201 attgagattt cgtgtagggg atgcgatcca acgctcccct gttgcgagtt tgaactcctt
     1261 ataggtaccc taaagtaacg gtttgattgc gccatctttg atgtcaactc cgataccaaa
     1321 atcggtcttc ctgtccgggc aatctttcgg tggttcctgt atccacttgg cgttttcttt
     1381 tgcgtgtttg gccgtgtagg tgtcgtttcc gccaatgtcc aggaaaatac ctatgtttat
     1441 catagtgtct ctggttgttc cgccattgca tggcattggc ccgccatatt gccccagaga
     1501 aggtgtggcg ccaatgtcgt atgtgtcatc gccaccgata tttgcaagga tgcctatccc
     1561 attgttattt ccgcagccgg cgacaagtcc tgcataaact tttttgccac cttccattct
     1621 gaaacagttg tatgtgtcgt taccaccaag gtccacattg attcctaccg atgtgtcgtg
     1681 accaacacct attgaagtgg ccattgtcgc ctggtatgtg tcattacccg ccctttcctg
     1741 aaagtatgcc aggccaaagt gggcacatga accctgaacg taccaggtcc ctttgtaatt
     1801 gtcatttcct gtatagtccg acaggattcc cattccatgc cagtatgcga cgccttgtcc
     1861 aaaaactccg caagaataag aatcatcgcc acccatgtcc tggagaatgc caacaccacc
     1921 agacatcgaa tgcccgttgt caaattctgc ccggaggcca tatcctgcgc cctggcacat
     1981 tgaggtgttg tgctccttgc tctgcgccga tggatttatt atgtccgtgt cgttggcgat
     2041 ataagtatca tttccgcttt tgtcttgaag gaggccaatt ccctgtgtga acccgaatcc
     2101 ctgtgaggca aagtatgatt cgtaaacgtc ggttccttcc tgatcctgta atatcccaag
     2161 cccgaactcc gccgcaccct gtgaaaggtt gatcgagttg tagtggtcat cgcctttctt
     2221 gtccaccagc acgccgacgc caaaacttgc cgcaccctgg gagcactcaa tagcgttgta
     2281 gttgtcgtca ccttcggcat catagaggca agcaaagccg aacctgccag agcccataca
     2341 gttgtggaaa ttgtagtccg ttttgtaggt atcattgcct gagaggtcta ttgataccga
     2401 aacaggattt tccaggccat tgttgcctgc tacccagccc gtgtaagtgt cgtttccgcc
     2461 agtatcgagc aaaagaagtt gcttctggca atcgtgccgg tcatcgccat cgccagaaat
     2521 tgttattctc ccaagtggag tgtcaacctc aaatggtgat ggtttctctt tggccagaag
     2581 cgctattgtc gagtcaactg caagcgacat ttgcgctgca ccgtagtaca tgaaggatgc
     2641 atcgaatgcc agtcctgcat cgtaggcctc gattggccat ccctttgttt caccgagaat
//
LOCUS       DAOSRI010000228         2680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34560_length_2680_cov_3.049143, whole genome shotgun sequence.
ACCESSION   DAOSRI010000228 DAOSRI010000000
VERSION     DAOSRI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34560_length_2680_cov_3.049143"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1263)
                     /gene="hydE"
                     /locus_tag="PK717_06805"
     CDS             complement(208..1263)
                     /gene="hydE"
                     /locus_tag="PK717_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOR47403.1"
                     /translation="MARIARNMGFEDVLSWLLEEDPKKLEELWAEADRTRHECVGDDV
                     HLRGLIEITNICSRECAYCGIRAPNSNVVRYKMSAFEIVECARMARKLGYGTVVLQGG
                     EDISISPDWIALVIREIKNETNLAVTLSLGERPENELRLWKEAGADRYLLRFETSNEE
                     LFRLIHPSLPWKVSDRIEQLKTLARLGYEVGSGVMVGIPGQDYETLANDIMTFQGLDL
                     DMIGVGPYIPHPCTPLGDGSLKPVPKGAQVPNDELMTLKTVALTRLACPYSNIPSTTA
                     LATIDKESGRELGLMRGANIVMPNLTPPKYRVLYEIYPDKACANETAEQCSLCLEQRI
                     ESIGRQIGSGPGGRRKN"
     gene            complement(1264..2505)
                     /gene="hydF"
                     /locus_tag="PK717_06810"
     CDS             complement(1264..2505)
                     /gene="hydF"
                     /locus_tag="PK717_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOR47404.1"
                     /translation="MFTSPRGIRLGIGIFGRRNVGKSSLLNAITRQSVSIVSSQAGTT
                     TDPVEKPMELLPLGPVLFIDTAGIDDEGALGELRIKKTRTILDRVDLGIIVFDGESFG
                     GFEIGLFEELKSRNVPAIFVFNKTDQGEPSQTAIEFAKTNNTRLILTSATSGQGVPEL
                     RQAILDSAPDDFVNNPSIAGDLVGPGGLAVLVVPIDKEAPKGRIILPQVQTIRDLLDH
                     NAVAVVAKESELKQTLDRFTTKPDIVITDSQAFLKVASDTPDDVPMTSFSILFSRHKG
                     DLLTQVQGALAIDRLKGGDRVLIAEACSHHPIDEDIGTVKIPKWLTKYVGANLEFKNT
                     QGHDFPEDLSPYKLVILCGGCMFGRRETLNRILHCKKQNVPVTNYGLTIAFSLGIFER
                     ALRPFPSVHKVYTDYLENKSR"
BASE COUNT          613 a          708 c          632 g          727 t
ORIGIN      
        1 cgaggtaggt tctggcctct tcttcggtaa gttgtccaag ttctatcagg ttcataacta
       61 atatgataac aagttgcatt ggtttgtcaa gtgcataatg ccgataaaat atatatctaa
      121 tagatagttt agaaaagata actgctatta ttgcacacag ctgattggga tatgaaatgt
      181 tcgtaaataa ttctcaataa tataaaatca attctttctc ctcccgccag gccctgagcc
      241 gatctggcgg ccaatggact caatccgctg ttccaggcaa aggctgcact gctctgcggt
      301 ctcgttggcg caggccttgt caggataaat ctcgtacagc accctgtatt ttggcggcgt
      361 caggttgggc atcacaatgt ttgcgcccct catcagccca agctccctgc cagattcctt
      421 gtcgatggtg gcaagtgccg ttgtcgacgg gatgtttgaa tacgggcagg ccagccttgt
      481 gagcgccact gtttttagcg tcattaattc gtcatttggc acctgtgcac cttttgggac
      541 aggcttgaga gagccatcgc caagcggcgt gcatgggtgg gggatgtagg ggccgactcc
      601 aatcatgtca agatcgagcc cctggaatgt catgatgtca tttgcgagtg tctcgtagtc
      661 ctgcccgggg atgccgacca tgacgccact gccaacctca taacccagcc tggccaatgt
      721 ttttagctgt tcgatcctgt cggagacctt ccacgggagc gaaggatgaa tgagcctgaa
      781 gagttcttcg tttgaggtct cgaatctcag caaatacctg tcggcacctg cctctttcca
      841 caacctcaac tcgttttccg gcctctcgcc aaggctgagc gtaaccgcca ggtttgtctc
      901 attttttatt tccctgatga caagggcaat ccagtccggg gagattgata tgtcctctcc
      961 accttgcaaa acaaccgtcc cgtagccaag ctttctggcc attctggcgc actcgacaat
     1021 ctcgaatgcc gacatcttgt atctgacaac gttgctgttt ggcgccctga tgccacagta
     1081 tgcgcattcc ctcgagcaaa tgtttgttat ctctatgagc ccgcggaggt gaacatcgtc
     1141 gccgacgcac tcatgccttg ttctatcggc ctctgcccaa agctcttcca gcttttttgg
     1201 atcctcctca aggagccacg aaaggacatc ttcaaaaccc atattgcggg caattcttgc
     1261 catttaccta cttttgttct ccagataatc agtatacacc ttgtgcactg acgggaaagg
     1321 ccttagtgcc ctctcaaaaa tgccgaggct gaaggctatt gtcaggccat agttcgtgac
     1381 cggaacattt tgcttcttgc aatgaaggat gcggttcagg gtctcccttc tgccaaacat
     1441 gcacccacca caaagtatta caagtttgta gggggaaagg tcttccggaa aatcgtggcc
     1501 ctgggtgttt ttaaattcca aatttgcccc aacatatttt gtgagccatt tgggaatttt
     1561 gacagtgcct atgtcctcat ctatcgggtg gtgtgagcac gcctcagcga tgagcaccct
     1621 gtccccaccc tttaatctgt caatggcaag tgcaccctgc acctgggtca aaagatcgcc
     1681 tttgtgcctg gaaaaaagga ttgaaaagct cgtcatcggg acgtcatccg gcgtgtcgga
     1741 ggccactttg agaaaagcct gcgagtcggt tatcacaata tcaggttttg tcgtgaacct
     1801 atccagggtt tgcttcaatt cgctctcttt tgccacaacg gccacagcat tgtggtccag
     1861 taggtccctg attgtctgca cttgtggcag tatgatcctg cctttcggtg cctctttgtc
     1921 tatcggaaca acaaggaccg cgagcccacc tggtccaaca aggtcacctg caattgatgg
     1981 attgttgaca aaatcgtctg gtgcactgtc cagaattgcc tgcctgagct ccggaacccc
     2041 ctgccctgag gtggcagagg tcagaatgag tctggtgtta tttgtcttgg caaattcaat
     2101 tgcagtctgc gatggctctc cctggtcggt tttgttgaaa acaaatattg cagggacatt
     2161 cctggatttc agctcctcaa aaagcccaat ctcaaaacca ccaaacgact caccgtcaaa
     2221 aactatgatg ccaaggtcca ccctgtccag aatggtcctg gtctttttta tcctgagctc
     2281 gccaagcgca ccttcatcat cgatgccggc agtatcaatg aagaggactg gcccaagcgg
     2341 caacagctcc attggctttt caacggggtc tgttgtcgtt ccggcctgac ttgaaacaat
     2401 cgagaccgac tgtctggtga tggcgttgag cagtgaggac tttccaacgt tcctcctgcc
     2461 aaagatgcca atcccgaggc gtattcctct gggtgaagta aacactgtca aactccttaa
     2521 aaatttcttt tgtacaggtt aggtcgccct gatatggcta ctgtccctat cagcactcaa
     2581 aaaccatttt ctggacaata acaccatcga tggctttcag ggtttcctcc attttcagga
     2641 tgtcatgctc cttgccatag agctcaagaa ggatgagtcc
//
LOCUS       DAOSRI010000229         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34700_length_2672_cov_3.891097, whole genome shotgun sequence.
ACCESSION   DAOSRI010000229 DAOSRI010000000
VERSION     DAOSRI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34700_length_2672_cov_3.891097"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK717_06815"
     CDS             complement(<1..539)
                     /locus_tag="PK717_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOR47405.1"
                     /translation="MNKKTILFLLAFCIFFGSLPYVQSKTIVKIYQATNIRMSPSVDA
                     KVLARIQPPKDLTVISEIKDKSGKVWFEVEASPGKKGFVASWVVKIQRTGTEEKIDGQ
                     VAVIDPNSGVINLRSSPNTSSSVALQIKTKTELPIVAKTKGDDEKDWYKLSLGNGRFG
                     WIGAGLIDEIKSPQVAKEEV"
     gene            complement(536..994)
                     /gene="smpB"
                     /locus_tag="PK717_06820"
     CDS             complement(536..994)
                     /gene="smpB"
                     /locus_tag="PK717_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOR47406.1"
                     /translation="MIKTGTISTNNKARHLYKLEDEIEAGIVLNGDEVKSLRMGHCDL
                     KDSFVEVVNGQAYVIDMNIPQYAMSHLGSNPKRKRKLLLHKLQIRRLAAKTLQGGYTC
                     VPISIYFNDKGFVKIRIAIAHGKKLFEHKDQIIRRDNERELSRAIKQRET"
     gene            1012..1575
                     /locus_tag="PK717_06825"
     CDS             1012..1575
                     /locus_tag="PK717_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47407.1"
                     /translation="MFGGKSRFCDFCYNLVVPLEYELINLLEGDCRIELTKVLSQKDF
                     DSVLRVPMHSIVSLNAANVSQYFNYPKTIPVAFRRKKTIEAFIVGVPVEKYLDDETFP
                     DPDIGRRNTIYSAIIAYSDTRTYNKLVADYRLYLSKFNATFESRHLRTDWAKGSEFEI
                     VKVFDYWKIDGVPVAYCKHKFAESSKK"
     gene            complement(1583..>2672)
                     /locus_tag="PK717_06830"
     CDS             complement(1583..>2672)
                     /locus_tag="PK717_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47408.1"
                     /translation="TASDMGKFLADLFKQERFFSEDSAYLFTSIAFEQDAVFYPKHTL
                     AFEVTKHDYKEPFPVLDKQGGALGVGSRLVVLPKQKIGLFAAEAGSNACHGFINQLLQ
                     KIWPPASEDDTSSEEGQNAKVEEPSRYTGYYSNMWYVQKNSFAKLGIIIFHDLYHFTY
                     NKDGYISINEWEYVPVGKDLFKSKLGDSYIKFVTDGNNQISHAQIWGQALERIDWFAS
                     PTPNLVFIIIFLLIVLSTIVSFIFGALLRKFDKTKQTKFDKLARWGQLVTALLYVSFL
                     VVVAIIFWGTNMIDYGFGIPLYMKATVLLPMIGALMTVALVYFTVMLWVKKYWSMTSR
                     IFYTVATFVYVLMIPWMIYWNLFSLIVA"
BASE COUNT          693 a          591 c          562 g          826 t
ORIGIN      
        1 acttcttcct ttgctacctg gggggatttg atttcatcaa tcaaccctgc gccgatccag
       61 ccgaaacggc cgtttcccaa cgaaagtttg taccagtctt tttcatcgtc gccctttgtt
      121 ttggcaacga ttggcaattc tgtttttgtc tttatttgca aggccacact cgaagacgta
      181 tttggagacg acctcaagtt tataacgcca gaattggggt ctataacagc gacctggcca
      241 tcaatttttt cctcggtgcc tgtcctctgg attttgacca cccatgatgc cacaaagcct
      301 tttttgcctg gcgaagcctc gacttcaaac cataccttac cggatttgtc ttttatttcg
      361 gaaattacag taagatcttt tggtggctgt attcttgcga gcactttcgc atcaaccgac
      421 ggcgacatcc ttatgttggt tgcctggtat atcttgacta tggtttttga ctgaacgtag
      481 ggcagggaac caaaaaagat gcagaaagct agcaaaaaca aaattgtttt tttgttcatg
      541 tctccctttg ttttattgcc ctggagagtt ccctttcgtt gtctctcctg attatctgat
      601 ctttgtgctc aaataatttt ttgccatgcg caattgctat cctgattttg acaaaaccct
      661 tgtcgttgaa ataaatcgaa atgggcacgc aggtataacc accctggagt gtttttgctg
      721 cgagcctcct gatttgcagt ttatgaagaa gaagttttct ttttctcttt ggattgctgc
      781 ccagatgaga cattgcatat tgtgggatgt tcatgtcaat aacgtaggcc tggccgttta
      841 caacctcgac aaatgaatct ttgaggtcac aatggcccat tcgcagcgat ttgacttcat
      901 cgccatttaa cacaatccct gcctctattt catcttcgag cttatacagg tgccttgctt
      961 tgttgtttgt ggaaatggtc cctgtcttga tcatttagat caattctatc tttgtttggt
     1021 ggcaagtcaa gattttgcga tttctgctat aatctggttg tgcctttaga atacgaactg
     1081 atcaatttgc ttgaaggaga ttgcaggata gaactcacca aggtcctttc gcagaaggat
     1141 tttgattctg tgctgcgtgt ccccatgcac tcgatagtct cgctcaacgc tgcaaatgtt
     1201 tcccaatact tcaattaccc caaaacaatt ccagttgcct tcaggagaaa aaagaccata
     1261 gaggccttta ttgttggcgt acctgtcgaa aaataccttg atgatgagac tttccctgac
     1321 cccgatattg gcagaagaaa taccatatat tctgcaatta tcgcctactc agacaccaga
     1381 acatacaaca agctggtggc agactacagg ctgtatcttt caaagttcaa cgcgacattt
     1441 gaatcaaggc acttgcgcac tgattgggca aaagggtcgg agtttgaaat cgtaaaggtg
     1501 ttcgattact ggaagattga cggtgttcca gttgcctatt gcaagcacaa gttcgccgag
     1561 agttccaaaa agtagcctac tgttaggcaa ctataaggct aaacaggttc cagtaaatca
     1621 tccatggaat catgagcaca taaacgaatg tggccacggt atagaatatc ctggaagtca
     1681 tactccagta ttttttcacc caaagcatca cagtgaaata gaccaatgcg acagtcatta
     1741 atgcgccaat catgggaagc aggacagttg ctttcatgta aagtggtatc ccgaagccat
     1801 aatctatcat gtttgttccc cagaagataa tggccacaac aaccaggaag ctcacataga
     1861 gcagtgccgt gacaagctgg ccccaccttg ccagtttgtc gaactttgtt tgctttgttt
     1921 tgtcaaattt tctgagcagg gcaccaaaga taaaggagac tattgtcgac aagacgataa
     1981 gaaggaatat tatgataaag acaagatttg gtgtcgggct tgcgaaccag tcgattcttt
     2041 caagggcttg gccccaaatt tgggcgtggc tgatttgatt gttcccgtct gtaacaaatt
     2101 ttatatatga atcaccaagt ttggatttga acaaatcttt tcctacaggc acgtattccc
     2161 actcattgat gcttatatag ccatccttgt tgtaggtgaa gtggtataaa tcatgaaaga
     2221 tgataatgcc caattttgca aaagaatttt tttggacata ccacatgttc gagtagtatc
     2281 cagtatatct tgagggctcc tcaaccttcg cattctgacc ttcctcactg ctagtatcat
     2341 cttcgcttgc tggcggccat attttttgca gcaattgatt gataaaacca tggcatgcat
     2401 ttgatccagc ttctgcggca aagagtccaa ttttttgttt tggcaatacc acaagacggc
     2461 tgcctactcc cagtgccccg ccttgtttgt cgaggaccgg gaatggttct ttgtaatcat
     2521 gctttgttac ttcgaatgcc agtgtgtgtt ttgggtagaa aacagcgtct tgctcaaatg
     2581 caatgcttgt aaacaggtaa gctgagtctt cggagaaaaa tctctcctgt ttaaaaagat
     2641 ctgccaggaa ttttcccata tcactggctg tt
//
LOCUS       DAOSRI010000230         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34969_length_2658_cov_3.492124, whole genome shotgun sequence.
ACCESSION   DAOSRI010000230 DAOSRI010000000
VERSION     DAOSRI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34969_length_2658_cov_3.492124"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /gene="rhuM"
                     /locus_tag="PK717_06835"
     CDS             <1..281
                     /gene="rhuM"
                     /locus_tag="PK717_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOR47409.1"
                     /translation="MHMADWTKKLDAFLEFNERNILENAGNISHQLAEEHARAEFDKY
                     NAERRKLEAESPQSDFDIMVEEAKKLEKSSPSPRKPRKSARQKKDGEK"
     gene            278..1663
                     /locus_tag="PK717_06840"
     CDS             278..1663
                     /locus_tag="PK717_06840"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOR47410.1"
                     /translation="MSAEQNKNAKLEEVDVAPSSISVFGLTENTIPKNWILGQLDTLA
                     YINPDSICSTTYTYSEIAYLDITNVTSGAIGKPITLPLKDAPSRAKRIVKHEDVILST
                     VRPGNRAYAFIKHAPNNLIVSTGFAVFRAKRGISEPRFIYYLISSNPIINYLASVAEE
                     KTAYPSVNPDDIAECIVPIPPLEEQKAIAHILGTLDDKIELNRRTNETLEAMAQALFK
                     FWFVDFGPVRAKMEGRWRRGQSLPGLPAHLFDLFPDRLVDSELGEIPEGWRISHIIDE
                     FNIIMGQSPPGKTYNDDGKGLPFYQGCSDFSFRFPIRRIYCTAPSRFAKKGETLVSVR
                     APVGDINMASEDCAIGRGVAAACHKTGNQSYTYQFMRALNGKLLCFEAEGTVFGSIGK
                     KDFNAITCLAPPNNLVIYYEKELSSIDKCIEINEYQSRTLTVLRDTLLPKLISGELRV
                     KQTREEQHNGD"
     gene            1653..>2658
                     /locus_tag="PK717_06845"
     CDS             1653..>2658
                     /locus_tag="PK717_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOR47411.1"
                     /translation="MGIEPIESLIFAIQSNPGTYALLLGSGISRAANIPTGWEITEDL
                     IQRLAVLKKEKCEPDPETWYCEKFGKEPDYSKLLEELAKTKTERQQLLRSYFEPNSAN
                     NEGDKRPTKAHKAIASLVAKGFIKVIITTNFDRLLENALDELGVKPTVISTPDQINGA
                     LPLTQNKSCQILKINGDYQDNRIRNTPSELEEYPVEFNKMLDQIFDEYGLIVCGWSAE
                     WDTALCDALSRVKTRRFTTCWAIRGELKEQASKLIGLRDAQKIEIKDADTFFQEIADG
                     VDAIEKYSKPHPLSTELAVANMKRYLSKSEYDIQFGDLVDETVNKIVEAISGEDFNVK
                     P"
BASE COUNT          858 a          556 c          596 g          648 t
ORIGIN      
        1 caatgcacat ggccgactgg acaaagaagc tggacgcttt tctcgaattc aacgagcgca
       61 atattttgga aaatgccggc aacatctcgc accagttggc agaagaacac gccagggcag
      121 aatttgacaa atataatgca gaaaggcgca agctggaggc agaatcaccg caaagcgatt
      181 ttgacataat ggttgaagag gcaaaaaagc tggagaaatc gtcaccttca ccccgcaagc
      241 ccaggaaatc ggccaggcaa aaaaaagatg gtgaaaaatg agcgcagaac aaaataaaaa
      301 tgcaaagctg gaagaggttg atgtggctcc ttctagcatt tcagtatttg gacttacaga
      361 aaatactatc cctaaaaact ggatacttgg tcaattggat acccttgcgt acataaatcc
      421 tgatagcata tgtagtacta cctataccta ctcagaaatc gcttacctag atatcaccaa
      481 tgttacatcg ggagcaatag gaaaaccaat aacattgcca ctcaaagatg cgcctagtag
      541 agcaaagcgt atcgttaagc atgaagatgt tattctttct acagtacgtc ctggaaatag
      601 agcatatgca tttataaaac atgcccccaa taatttaatt gtatctacgg ggtttgctgt
      661 atttagagct aaaagaggaa tctccgaacc gagatttatt tattatttaa tctcaagcaa
      721 tccgattata aattacttag catctgtggc agaagaaaaa acagcttatc cgtccgttaa
      781 tccagacgac atagcagaat gcatagttcc tattcctccg ctggaggagc aaaaagcgat
      841 agcgcacata ctggggacgc tggatgacaa aatcgagcta aaccggcgga caaacgaaac
      901 gctggaggcg atggcacagg cgctgttcaa gttctggttt gtggacttcg ggccggtgcg
      961 cgccaaaatg gaggggcggt ggcgccgcgg ccagtcgctc cccggtctcc cggcccatct
     1021 ttttgacctt ttcccggacc ggcttgtgga ctccgaactc ggcgagatcc ccgaaggctg
     1081 gaggataagc catattattg atgaatttaa tataataatg ggacaatctc caccaggtaa
     1141 aacatataat gatgacggca aaggattgcc attttatcaa ggttgctcag attttagttt
     1201 ccgttttcca attcgccgta tttactgtac agcaccaagc aggttcgcta aaaaaggaga
     1261 aacactcgta agcgtaaggg cacctgtggg cgatattaat atggcttcgg aagattgtgc
     1321 tataggacgt ggtgttgcag ctgcttgtca taaaacaggc aatcagtcat acacatatca
     1381 atttatgcgt gcattaaatg ggaaacttct atgttttgaa gctgaaggta cagtttttgg
     1441 atcaattggg aaaaaagatt ttaatgccat aacttgttta gcaccaccga ataatttagt
     1501 tatctattat gaaaaagaat tatcttcgat tgataaatgt atcgaaataa atgaatacca
     1561 atctcgcact cttaccgtct tacgtgacac actgttgcca aaactcattt ccggggaatt
     1621 gcgggtaaaa caaacgaggg aggaacaaca caatggggat tgaaccaatc gagtcgctaa
     1681 tttttgcaat ccagtccaat cctggaacat atgcgttact gcttggatct ggcatttcca
     1741 gggctgccaa cattccaaca ggctgggaaa taacagaaga tttgatacaa aggctagctg
     1801 ttctaaaaaa agagaaatgc gagcctgacc ctgaaacctg gtattgtgag aaatttggca
     1861 aggaaccaga ctattcaaaa ttattagagg aacttgcaaa aactaaaact gaaaggcagc
     1921 agcttttgcg gtcatatttt gaacccaaca gcgcaaacaa tgagggcgac aaacggccga
     1981 ccaaagccca taaagccatt gcctcactgg ttgcaaaagg tttcataaaa gtcatcataa
     2041 caacaaattt tgatcgcttg ctggaaaatg ccttggatga acttggtgtc aagcctacgg
     2101 tgataagcac acccgatcaa atcaatggtg cattgccatt gacgcaaaac aaaagctgcc
     2161 aaatccttaa aatcaacggc gattaccagg acaatcgaat tagaaacaca ccctccgagc
     2221 ttgaagaata tcctgtcgaa ttcaacaaga tgcttgacca gatttttgat gaatacggcc
     2281 ttatagtgtg tggctggtct gcagagtggg acactgcatt atgcgatgca ctgtcgcgtg
     2341 tcaaaacaag gcgttttaca acatgctggg cgattcgtgg ggagcttaaa gagcaggcca
     2401 gcaaattgat tgggctacgc gatgcgcaaa aaattgaaat caaggatgca gatacattct
     2461 ttcaggaaat tgccgatggc gttgatgcca ttgagaaata ttcaaaaccc catccgctct
     2521 caaccgagct ggccgttgca aatatgaaac gctatctttc caaatctgaa tatgacatcc
     2581 agtttggaga ccttgttgat gaaacagtga acaagattgt tgaagcaata tcaggcgagg
     2641 atttcaatgt aaaaccta
//
LOCUS       DAOSRI010000231         2657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_34990_length_2657_cov_3.939662, whole genome shotgun sequence.
ACCESSION   DAOSRI010000231 DAOSRI010000000
VERSION     DAOSRI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34990_length_2657_cov_3.939662"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PK717_06850"
     CDS             complement(<1..711)
                     /locus_tag="PK717_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOR47412.1"
                     /translation="MEEKISKMKTSHESDASVRLWLERKAIRTGEFDPDFLRESHLRE
                     WILPSLTAFKQQGLIEDVIMEVSSGKEATVYSCLGTKKSGFPLLAAKVYRPRIFRSLS
                     NDALYREGRWVSQKSVQRAINRRSTMGHQFMFERWIEDEFSVQRLLWNNGVDVPKPIA
                     IKGNTILMEYIGDENGPAPLLRKVKIPDKDMDRAFRRMLTNIRNMLGLNVVNGDLSAF
                     NVLWWGGKAIIIDMAQAVD"
     gene            complement(687..815)
                     /locus_tag="PK717_06855"
     CDS             complement(687..815)
                     /locus_tag="PK717_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47413.1"
                     /translation="MKNGKGFKVMKLTQIKPKMTPKITVPKAKNSKEEFWKKKSPK"
     gene            complement(819..962)
                     /locus_tag="PK717_06860"
     CDS             complement(819..962)
                     /locus_tag="PK717_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47414.1"
                     /translation="MTTLFDKLAQALTPAVVRAYKIKLERYEGGKVSAAGKADQTNEE
                     GRH"
     gene            1266..2522
                     /locus_tag="PK717_06865"
     CDS             1266..2522
                     /locus_tag="PK717_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOR47415.1"
                     /translation="MSEPLAYRMRPQTIEEFVGQKHLLGKDGLLSKSLSGKPTSLLLW
                     GPPGTGKTTLAHLIGKATSRQTIVINAVTTGIPDLKKLLAQADHPVLFVDEIHRWSKS
                     QQDFLLPFVESGEVLLVGSTTENPYYEVIGPLLSRLRVLRLEPLSDEDIKEILKKAII
                     DPKGLGEFTIEMPEEVMDFVAQVSAGDARSALNLLEDLFNLSVPVDGKYTLQIPSEDV
                     LRRLPYDKTGDEHYQVISAFIKCIRGSDPDAAVYWLSRMLKSGEDPTFIARRLVILAA
                     EDIGLADPWALLIAQSGFSAVEQIGIPECGLILSEVAVYLAQAPKSNSTARALWAAQE
                     TVEKTTPPVPIHLRNANFRGLKNLGWGRDYKYPHDHKSGFVKQEYLPKELLDSKFFEP
                     TDIGFEKRIKERMESRGQVEKDDKNE"
BASE COUNT          623 a          686 c          649 g          699 t
ORIGIN      
        1 gtcaacggct tgcgccatgt ctatgatgat cgccttcccg ccccaccata gcacgttgaa
       61 tgcggaaaga tcgccgttga caacattgag cccgagcatg ttcctgatgt ttgtcagcat
      121 ccgcctgaat gccctgtcca tgtccttgtc tggaattttc actttcctca aaagcggtgc
      181 tggcccgttc tcatcaccga tgtattccat gagtattgtg ttgcccttaa tcgcaattgg
      241 cttcgggaca tcaacgccat tgttccagag gagcctctgg acgctgaatt cgtcctcaat
      301 ccaccgctcg aacatgaact ggtggcccat cgtggacctc ctgtttatgg ccctttgcac
      361 gcttttctgg gaaacccacc tgccctccct gtagagggca tcattgctca ggctccggaa
      421 gatccttggc ctgtaaacct tggccgcaag gagcgggaac cctgactttt ttgtccccag
      481 gcaactgtag actgtcgcct ccttgcccga tgagacttcc ataatcacgt cttcgatcag
      541 cccctgctgt ttgaaagcag tcagtgaagg cagtatccac tcgcgcaggt ggctctctct
      601 caggaaatct gggtcaaatt caccagtcct gatggccttt ctttcgagcc agagccggac
      661 tgaagcgtcc gactcgtggc ttgtcttcat tttggagatt ttttcttcca aaactcctcc
      721 ttggaatttt ttgcttttgg caccgttatt tttggtgtca tctttggttt tatctgcgtc
      781 aacttcataa ctttgaatcc tttgccattt ttcataaatt aatgcctccc ttcttcattt
      841 gtttgatcgg cttttccagc cgctgacact ttcccgccct cgtatcgttc gagctttatc
      901 ttgtatgcgc ggacgacggc cggtgtgagt gcttgtgcga gcttgtcaaa tagcgtggtc
      961 attgtgttcc tcctttgcgg gaaattaaaa aggccccttt gcctttttgt gcgctggggc
     1021 cctggaatta aaaaggcccg ttccctgttt tgaacgggcc ttctagttta ttttatggtt
     1081 tggtattgct tagactgagg ctccgcccaa aagatgcgca ttgttgttat tgtttctcac
     1141 tctcatcatc gcacctcctt tggcggaatt tgttttgagg tgattctagg cgtattctca
     1201 aggttgtcaa gacgggcgct ctggcaagtg ttgaaaaata ccggtttgtc caatacaatt
     1261 gccgcatgtc agaaccgctt gcttacagga tgaggccaca aacgatagaa gaatttgttg
     1321 gccagaagca tctgctcgga aaagacggcc tgctctcaaa atccctgtcg ggcaagccaa
     1381 cttcgctttt gctgtggggg ccacccggaa caggtaaaac cacacttgcg cacctgatcg
     1441 ggaaagcgac atcaaggcaa acgattgtca tcaatgctgt aacaacaggc attccagacc
     1501 tgaaaaaact gcttgcgcag gccgaccacc ctgtcctttt tgtggacgag atccacagat
     1561 ggtcaaagtc ccagcaggac tttctgctcc cctttgtcga gtctggcgaa gtcctgctcg
     1621 tcggctccac aacagaaaac ccttactacg aggtaattgg cccgctcctc tcaaggctca
     1681 gggtgctgag gctggagcca ctctccgacg aggacataaa ggaaatcctc aaaaaagcaa
     1741 tcatcgatcc aaagggcctc ggtgagttta ccatagaaat gccagaagag gtgatggact
     1801 ttgtcgccca ggtgtcggca ggagatgcga ggtcggcgct caaccttctg gaagaccttt
     1861 tcaatctctc ggttcctgtg gatggcaaat atacccttca gataccatca gaagatgttt
     1921 tgcgaagatt gccgtatgac aagacaggtg atgagcacta tcaggtcata tcagccttta
     1981 taaagtgtat caggggctca gacccagatg cggccgtcta ctggctttca aggatgctga
     2041 agtccgggga agatccgacc ttcattgcaa ggagactcgt cattctggcc gcagaggaca
     2101 tcgggcttgc agatccatgg gcattgctga ttgcccaatc aggattttcg gccgtcgaac
     2161 agataggcat cccggaatgc gggttgatcc tgtccgaggt ggctgtgtac ctggcgcagg
     2221 ccccaaaaag caacagcacg gccagggcgc tctgggccgc acaggaaacg gtcgagaaaa
     2281 caactccgcc ggtgccaatc catctcagga acgctaattt caggggactg aaaaaccttg
     2341 gctggggcag agactacaaa tatccacacg accacaaaag tgggtttgtg aaacaagaat
     2401 atctaccaaa agagctgctg gattcgaaat tttttgagcc aacagacatc ggctttgaaa
     2461 aacgcatcaa ggagcggatg gaatccaggg ggcaggttga aaaagatgac aaaaacgaat
     2521 gaaaataaac tgatggctat ctatgctgtc ttttttacat tccaggtgct ccacattttt
     2581 gaggaaatct ggggcagaac ttatgaaata cctatcttgc cattccacag gcttgaaaat
     2641 tacctgattg cagcatc
//
LOCUS       DAOSRI010000232         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_35305_length_2639_cov_4.457817, whole genome shotgun sequence.
ACCESSION   DAOSRI010000232 DAOSRI010000000
VERSION     DAOSRI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35305_length_2639_cov_4.457817"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PK717_06870"
     CDS             <1..618
                     /locus_tag="PK717_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR47416.1"
                     /translation="TTDQERLIEIRANVGLVFQNPDNQTVASLVEDDVAFGPENLNLP
                     PEEIRRRVDEALSIVGLSHLLKHPVHHLSGGQKQLVAIAGILAIKPKLLILDEATSLI
                     DPKGRKQVLEKIKQLHKDGYAIVMITHRMEEVLLSERSIALEKGSMIFDGKPEDLFSD
                     ASLLARAGLERPPLVQLVESLSDNGVEVPFPKDENELVEALCQLK"
     gene            603..1448
                     /locus_tag="PK717_06875"
     CDS             603..1448
                     /locus_tag="PK717_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOR47417.1"
                     /translation="MPIEVKNISYIYDQGMPFEKKALDDVSITIKDASITGIIGSTGS
                     GKSTLIQHFNALLVPTMGEIVVDGIETKKWDKKALRRKVGLVFQYPEAQLFGETVFAD
                     IAFGPQKQGLKPDEVEERVLHALKLVGLDPSAYRNRSPFKLSGGEKRKVAIAGVLACH
                     PEIIVLDEPTAGLDPVSRKAFLELVVGFKKEGHTLVMVSHDMDEVAGIAQNVIIMGDS
                     RVIFEGPTDEAFSQPDLLRSAGLDVPALSSVAHKLRNAGFSLPRKTFDARELAKVIAG
                     QLSKQ"
     gene            1445..2248
                     /locus_tag="PK717_06880"
     CDS             1445..2248
                     /locus_tag="PK717_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOR47418.1"
                     /translation="MSLANFVTIGQFVPLDTPIHRLDPRVKLMLSLVLLVFIFIIGKL
                     LPLLALFVLAMICAALAKVPFMHLVRGMRPILILIVLTVVIQLFSTSGEPLLSFWGLT
                     ITKEGILVSLFVVLRLILLVSISVILTYATSPLDLTFALESFMLPLKRIGFPASEIAL
                     MMSIALRFIPTLLEETDKIIKAQTSRGADFSSGGIIARAKSFIPVLIPLFVSAFRRAD
                     ELADAMESRCFITGEKRSRLHVPKLGLNDFVAVALCVIAMAGVYVWSIL"
     gene            2229..>2639
                     /locus_tag="PK717_06885"
     CDS             2229..>2639
                     /locus_tag="PK717_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR47419.1"
                     /translation="MSGQSFNYKAVISYDGSAFFGWQRQPGLPTVQGTMEDSIFEALD
                     EETYVYASSRTDKGVHALGQVISFKLPKKFNLVGMKSLLDSQLPPTIRIRSLDYAPEN
                     FIARFESRKKVYSYIAHLGPSPSPFVHNGSWVIDD"
BASE COUNT          700 a          612 c          608 g          719 t
ORIGIN      
        1 accaccgacc aggagaggct gattgagata agggcgaatg taggccttgt ctttcagaac
       61 ccggacaacc agactgttgc atcacttgtt gaagacgacg ttgcttttgg cccggaaaat
      121 ctcaatctcc cacctgaaga aatcagaaga agagttgacg aggctttatc aattgttggc
      181 ctttcgcatt tactaaaaca tcctgtacac cacttatctg gtggccaaaa acagcttgtt
      241 gcaattgcag gtatcctcgc aataaagcca aaattgctga tccttgatga ggcaacatct
      301 cttattgacc caaagggcag aaaacaggtg cttgaaaaaa tcaagcaact gcacaaagat
      361 ggctatgcaa tcgtcatgat cacacacagg atggaagagg tgcttctctc ggagagatca
      421 attgcccttg agaagggcag catgattttt gatggcaaac cagaggacct tttttctgat
      481 gcctccctcc ttgctcgcgc cgggcttgaa aggccacccc ttgtgcaact tgttgaaagc
      541 ctgtctgaca atggtgtaga ggtcccattt ccaaaagatg aaaacgagct tgtggaggcc
      601 ctgtgccaat tgaagtaaag aacataagct acatatatga tcagggtatg ccatttgaaa
      661 aaaaagccct ggatgatgtc agcatcacga taaaagacgc cagtatcact ggaataattg
      721 gctcgactgg ctctggaaaa agcaccctta tccagcattt taacgccttg cttgtcccca
      781 ctatggggga aatagtggtt gacggtattg aaacaaaaaa atgggacaaa aaggccctga
      841 gaagaaaagt gggacttgtt ttccagtatc cagaagctca gctttttgga gagaccgttt
      901 ttgcagatat tgcttttggc ccacaaaaac agggactgaa acccgatgag gttgaagaga
      961 gggtccttca tgcgctcaag ctcgtgggtc ttgacccttc tgcatacaga aaccgttcgc
     1021 cattcaaact ctctggtgga gaaaaaagaa aagttgcgat agcaggtgtt ttggcctgcc
     1081 atcccgagat aatcgttctc gatgaaccga cggcagggct tgatccagtt tccagaaagg
     1141 ctttcctcga gcttgttgtt gggttcaaaa aagaaggtca cacccttgtc atggtttcgc
     1201 atgacatgga tgaagtggcc ggtattgccc agaatgtcat catcatgggt gattcaagag
     1261 tcatctttga aggtccaaca gatgaagctt tttcgcagcc agatttgttg aggtctgccg
     1321 gccttgatgt tccagcgctt tcctcagtgg cccacaaact gagaaatgca ggattttctc
     1381 tcccaagaaa aacattcgat gcacgggagt tggccaaagt catcgctggc cagttgagca
     1441 aacaatgagc ctggcaaatt ttgtaacaat tggacagttt gtcccacttg acaccccgat
     1501 acacaggctt gatccaagag tcaagctcat gttgtcactt gttctccttg ttttcatatt
     1561 tattattgga aaacttttgc ctttgctggc gctttttgtt cttgccatga tttgtgcggc
     1621 attggcaaaa gtacctttta tgcaccttgt cagaggcatg aggccaatat tgattcttat
     1681 tgtgctcacc gttgttattc agttattttc tacgagtggc gagccactcc tgagtttctg
     1741 gggtctgaca atcaccaaag agggcatact tgtgtcactt tttgtagtcc tcaggctcat
     1801 tctgcttgtt tcgatttctg taatactcac ctatgcaaca tcgccacttg atctgacttt
     1861 tgccctggag agcttcatgc taccgctcaa acggatcggt tttccggcct cagagattgc
     1921 cctcatgatg agcattgcat tgaggtttat tccaacactc cttgaggaaa ctgacaaaat
     1981 cataaaagcg caaacatcca gaggggccga tttttcatcg gggggcataa ttgccagagc
     2041 aaaatctttt attcctgtcc tcataccgct ctttgtttca gccttcagaa gggccgacga
     2101 gctggctgat gccatggaat caaggtgttt tatcaccggc gaaaaaagat caaggctcca
     2161 tgtgccaaaa cttggcctaa acgattttgt tgcagtcgca ttatgcgtaa ttgcgatggc
     2221 tggcgtttat gtctggtcaa tcctttaact acaaagcagt catcagctac gatggctccg
     2281 catttttcgg gtggcagagg caaccaggcc ttccgaccgt ccagggaaca atggaagact
     2341 ccatttttga ggcacttgac gaagaaactt acgtttatgc ctcatcaagg accgacaagg
     2401 gcgttcatgc gcttggccag gtcatttcat ttaaactccc aaagaagttc aacctggttg
     2461 gaatgaaaag tcttttggat tcacaacttc caccaaccat aagaataagg tcactcgatt
     2521 acgctcctga gaattttatt gccaggtttg aaagcaggaa aaaggtctac tcatacattg
     2581 cccatcttgg cccttcccct tccccttttg tccacaatgg ctcctgggtc attgatgat
//
LOCUS       DAOSRI010000233         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_35351_length_2636_cov_4.098024, whole genome shotgun sequence.
ACCESSION   DAOSRI010000233 DAOSRI010000000
VERSION     DAOSRI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35351_length_2636_cov_4.098024"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="PK717_06890"
     CDS             <1..1648
                     /locus_tag="PK717_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47420.1"
                     /translation="EKSSKLIENFIVSSLPSYAMVKIDNIEITSPKRLLLEGGYHKVC
                     LFSGQTQVICTDVVLPTVGKPVIISWPDNITSPCLYFGEGLYPLPYDVRNITVSPDGS
                     RLLFNTQASYVNCVDLITGRELWATKLDRAFNGLPAMILGADDEKVYGSAGIPPGLNW
                     GGPGSTAFAISLETGSEIDIDKLYSGSALPMSPPKFQAGGSQFYGHVWKGPVQPNLSI
                     PKGMEAVVVTGNEVKRFTHDVDYWDEADFLGVSVSAKKNWPIFVYQTIQKGYGSGFVD
                     VHVLDTQDAKPIKTGEEDEKARYYEGSWINFSGPFPAKGVCFDGGFDTKGTFIMWNDH
                     QIASVAYPSGKIVWKKYVDNVPSQPPTIISAKGKPVVLLNFKVSPYELQFDLYTGEQI
                     VARSKPQDAAEAIGGEACPGGVFVLPKGKIVSGVKSDANGNFKPTWVKVYDDVLVKAS
                     PWGALVILNNTVTLLGSHELAPVMSIKLPGLGSPKEAQVFGNQNDLAIYAGTSCWIVD
                     RYGLVKGYFANVSSLHALESNGHKALLATIGQRQVVIPWP"
     gene            1645..>2636
                     /locus_tag="PK717_06895"
     CDS             1645..>2636
                     /locus_tag="PK717_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47421.1"
                     /translation="MKWWKVVLFVLGGVLVIGAVIFILLFGGPKGSVNVVSGSPVKLI
                     FDDADYGEGTSFSARLPAGNHKLEATLVGVAGNIPIITKYFEVVKDRDLTIEIAMDPT
                     VEFSSVPQGADVFVEMDGQKRLGKTPFSIQMPTGDYKFTFVLNGAKIERGPYKLEMDS
                     NQTIDAYYGKAPRTGLDEKDKFFISSWPDLMRVYENGELVGITPVSFPKQGIFEVWSG
                     ESSISLPVLNQDTQYFFVDQGTGGLGYAFSKKQIVSAKSDFFTSTGSFVAWVDGVTVR
                     VKMEGAQDLVIPLPRFDSPLLLSSNSTTITFSGIFGSDIIIKTYEVPSGKEIPSP"
BASE COUNT          726 a          498 c          640 g          772 t
ORIGIN      
        1 agaaaaatca tcaaaactaa tagagaactt cattgtttca tcattgccgt catatgctat
       61 ggtgaaaatt gataatattg aaatcacctc ccccaaaaga cttttgcttg agggtggcta
      121 tcacaaggta tgcctttttt ctggtcaaac acaggtaatt tgcaccgatg ttgttttgcc
      181 tactgttggc aagccagtta ttatctcctg gcctgataat attaccagcc cgtgcctgta
      241 ttttggtgaa ggtctgtatc ccctgcctta tgatgtaagg aacattactg tttcgccaga
      301 tggcagcaga cttcttttca acacgcaggc aagctatgta aattgtgttg atctgatcac
      361 aggcagagag ctttgggcaa caaaactcga tagggcattt aatggacttc ctgcaatgat
      421 tcttggtgct gatgatgaga aagtatatgg ttctgcaggt attcctccag ggttaaactg
      481 gggtggtcct ggatccaccg cttttgcaat aagccttgag actggatcag aaattgatat
      541 tgacaagctt tatagtggat ctgcattgcc aatgagtccg ccaaaattcc aagctggtgg
      601 ttcacaattt tatggtcatg tatggaaggg tcccgttcaa ccgaacttgt ccataccaaa
      661 gggcatggaa gctgttgtag ttactggtaa tgaagtaaag agatttactc acgatgttga
      721 ctattgggat gaagctgatt ttttgggtgt ttctgtttct gcaaagaaaa attggcccat
      781 ctttgtatat cagacaatac aaaaaggcta tggaagtggt tttgtagacg ttcacgttct
      841 ggacacacag gatgcaaaac ccataaaaac tggcgaagaa gatgagaaag caagatatta
      901 tgaaggtagc tggataaatt tcagtggtcc tttcccggct aaaggggtct gttttgacgg
      961 agggttcgac accaagggta catttatcat gtggaatgat caccagattg cttctgtggc
     1021 ttatccaagc ggaaaaattg tctggaaaaa atatgttgat aatgtaccaa gtcagccacc
     1081 gacaataatt tcagcaaaag gaaaacctgt tgttcttttg aacttcaaag tctctcccta
     1141 tgagttgcag tttgatttgt acacaggtga acaaattgtc gccagatcaa aaccgcaaga
     1201 tgcggctgag gcaatcggcg gtgaagcatg tccaggtgga gtttttgtgt tgccgaaggg
     1261 taaaattgtt tctggagtca aaagcgatgc aaatggcaat ttcaaaccaa cttgggtcaa
     1321 ggtatatgat gatgtgctgg ttaaagcttc gccctgggga gcacttgtca tcctcaataa
     1381 tactgtaacg cttcttggaa gtcatgaatt ggcaccagta atgagcatta aacttcctgg
     1441 tttgggttca ccgaaagagg cccaggtttt tgggaaccag aatgatctgg ccatttatgc
     1501 agggaccagt tgctggatcg ttgatcgata tggccttgta aaagggtatt ttgcaaacgt
     1561 gtcatcgctc catgcgcttg aatcaaatgg acacaaagct ctcttggcaa cgattgggca
     1621 aagacaggtg gttataccgt ggccatgaaa tggtggaaag ttgttctatt tgttctgggt
     1681 ggcgtgctcg taattggcgc agttattttt attttgcttt ttggtggccc aaaggggagt
     1741 gtaaatgttg tttcagggag cccggtaaaa cttatttttg atgatgccga ttatggtgaa
     1801 ggaacatctt tttctgcgag attaccagca ggaaaccaca agcttgaggc aaccctcgtt
     1861 ggggtggctg gaaatatccc gatcataaca aagtattttg aggttgtgaa agacagagac
     1921 cttacaattg agattgccat ggaccccacc gtcgaatttt catctgttcc ccaaggtgca
     1981 gatgtttttg tggaaatgga tgggcaaaag aggcttggca aaactccgtt ttcgatccaa
     2041 atgccgacag gtgactacaa gtttacgttt gtgctcaatg gtgcaaaaat tgaaaggggc
     2101 ccatacaagt tggagatgga ctcaaatcag accattgatg cctactacgg gaaagcccca
     2161 agaactggcc ttgacgagaa agacaagttt tttatttcct cttggcctga tcttatgagg
     2221 gtctatgaga atggcgagct tgttggcata acaccagttt cttttccaaa acagggaata
     2281 tttgaagtat ggtctggtga atccagcatt tcattgcctg ttttgaatca ggacacacaa
     2341 tatttttttg tagaccaggg tactggtggc cttggctacg cgttctctaa aaaacagatt
     2401 gtttcagcaa aaagcgattt ttttacaagc acaggctctt ttgttgcgtg ggttgatggt
     2461 gtaactgtca gggtcaaaat ggaaggagca caagatcttg tcataccgtt gccaaggttt
     2521 gattcaccat tgcttctttc atcgaattcc acaacaatca ctttctctgg gatatttggg
     2581 agcgatatta taatcaagac ttatgaagtc ccgtctggga aagaaatccc gtcgcc
//
LOCUS       DAOSRI010000234         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_35439_length_2631_cov_4.526009, whole genome shotgun sequence.
ACCESSION   DAOSRI010000234 DAOSRI010000000
VERSION     DAOSRI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35439_length_2631_cov_4.526009"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1500
                     /locus_tag="PK717_06900"
     CDS             163..1500
                     /locus_tag="PK717_06900"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR47422.1"
                     /translation="MDNFGIRELEDDGITIRIPTFDNVPITGSVQKLMDTPQFQRLRK
                     IRQLSLAHLVYPGANHTRFEHSLGVYSLMRAYVSHLLNTGLTDFASKEDVLSLLAASL
                     LHDIGHYPFGHIVEGMATGLEFHETRGQQICQNLTMVNLLSDMGANPELTGRLISGDD
                     AGLPDNRKKTFRLLRDFLDCAIDADKMDYLERDSVHCGVPYGRSYDKERLINSLIISQ
                     DGRLALTEKGKASAEYVIFSRYVMLTEVYWHHTVRSASVMLIRAFQEAQGSSHWGDYL
                     HKLLENFGDEDALEALSALVRDDPDLSVLASGLRIRQLYKRIRTYSPLDPDSQKVFGT
                     LKGLEGPADGRALSIYVEKELGLPRARVLVDIVPAKSLAGDMMVYYPKRGQYRKLSSV
                     SPIASSLHEAWGSLTGIIRVYAHPSYAEKLASMGNELDMIIEKAGSDLRTKPK"
     gene            1497..2408
                     /locus_tag="PK717_06905"
     CDS             1497..2408
                     /locus_tag="PK717_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HOR47423.1"
                     /translation="MNILDTVGNTPLVDLTNSFCKNHGVCIMGKLEGANPGGSVKDRP
                     ALKMIKTAEQSGELKPGKTILEPTSGSTGIALAMIGAAKGYPVKLVMPECVSLERRLT
                     LQAYGAEVILTGACEGTDGAIKLARKMVEERPDVYYMPDQFSNPNNPLAHYETTGPEI
                     MRQTDGKIDVFVAGLGTTGTLMGTGKYLREKKSGVKIVAVEPTIGHKIQGLKNMAESM
                     VPQIYDKSFPDETIIVGDEEAFETTRLLAVKEGIFVGMSSGAALWGAIQVAKIMSGGM
                     IVVILPDKGDRYLSTNLFKSVCAKCPP"
BASE COUNT          782 a          617 c          661 g          571 t
ORIGIN      
        1 gagtgttagt atggcccagc ctcccccgaa agccagagag ccaatcttga aaaatgagac
       61 aaaaatctga acaagtgtgg ccatgcacaa atcttatgca aatgtccatt atttgacaag
      121 gtaaagcttg accttggcaa aacaccaaat agaatttgcc caatggacaa ttttgggata
      181 agggaactgg aagacgacgg gataacaatc aggataccaa cttttgacaa tgtcccgata
      241 actgggtctg tccagaaact catggacaca ccacaattcc agagactaag aaaaataagg
      301 cagctttctc ttgcacacct ggtatacccc ggggcaaacc atacaaggtt cgagcatagc
      361 cttggcgtat acagcctcat gagggcctat gtttcgcacc tgctgaatac cggcttgacg
      421 gattttgcat caaaagagga tgtactgtcg ctgcttgcag catcactcct ccacgacatc
      481 gggcattatc catttggcca catagttgaa gggatggcca cagggctcga atttcacgaa
      541 acaaggggcc agcagatctg ccaaaacctg acaatggtaa atctgctctc agacatgggt
      601 gcaaatcctg aattgacagg cagactgatc tctggcgatg atgcgggact cccagacaac
      661 aggaaaaaaa ctttcagact actgcgcgat ttcctggact gcgcaataga tgccgacaaa
      721 atggattacc ttgaaagaga ctcggtccac tgtggcgtgc cttatggcag gtcatacgac
      781 aaggaaaggc tcatcaattc actgataatc tcgcaagatg gcaggctggc gctaacagaa
      841 aaaggcaagg caagcgccga gtacgttatc ttctcaaggt atgtgatgct gaccgaagtc
      901 tactggcatc acactgtcag atcagcctcc gtgatgctca taagggcatt ccaggaagca
      961 cagggctcca gccattgggg tgattacctg cacaaactgc tcgagaattt tggtgatgaa
     1021 gacgcgcttg aagcgctctc ggcactggtc agggacgatc ccgatctctc ggtccttgcc
     1081 agtggcctca gaataagaca gttatacaag aggataagga cgtacagccc gctcgatcct
     1141 gattctcaaa aggtctttgg cacactcaag ggccttgaag ggccagcaga tggcagggca
     1201 ttgtcaattt atgtggagaa agaacttggc ctcccaagag caagggtgct ggttgacata
     1261 gttcctgcaa aatcgcttgc aggcgacatg atggtttatt acccaaaacg cggccagtac
     1321 agaaaactct caagcgtttc tccgatagca tcttcgctac atgaggcctg gggttccctg
     1381 accggcatca tcagggtata tgcccaccca agctacgccg agaagctcgc ctcaatggga
     1441 aacgaacttg acatgataat agaaaaagct ggttctgatt taaggacaaa accaaaatga
     1501 acatcctgga cacggtagga aatacacccc ttgtcgatct aacaaattct ttctgcaaaa
     1561 atcacggcgt ttgcataatg ggcaagctgg agggcgcaaa tccgggtggc tcggtaaaag
     1621 acaggccggc cctcaaaatg ataaaaacgg cagaacaatc tggcgaactg aaacctggaa
     1681 aaaccatact ggagccaaca tcaggaagca ccggtatagc gctggccatg ataggtgctg
     1741 caaaaggcta tcccgttaaa ctggtcatgc cggaatgcgt tagtctggag agaagactca
     1801 cccttcaggc gtacggcgca gaagtcatcc tgaccggggc ctgcgaaggg accgacgggg
     1861 cgataaaact ggccagaaaa atggtcgaag aaaggccgga tgtatactac atgccagacc
     1921 agttctcaaa tccaaacaat cccctggcgc actacgaaac cactggcccg gaaataatgc
     1981 gccagacaga tggcaagata gatgtatttg ttgccgggct tgggacaacc ggaacactca
     2041 tggggaccgg aaaatatctc agggagaaaa aatctggcgt aaaaatcgtt gccgtggagc
     2101 caaccattgg ccacaagata cagggcctca aaaacatggc agagtccatg gtgccgcaaa
     2161 tctatgacaa aagcttccca gatgaaacaa taattgtcgg ggatgaagag gcttttgaaa
     2221 caacgaggct cctcgcagta aaagagggta tttttgtcgg gatgtccagc ggtgcagcgc
     2281 tctggggcgc catccaggtg gcaaaaataa tgtctggtgg aatgattgtt gtcattctcc
     2341 ccgacaaggg cgacaggtac ctgagcacaa acctcttcaa gtctgtttgc gccaaatgcc
     2401 cgccatgatg caaatcttca aaatcaattg acaaaattgt aattttatta tcattggtac
     2461 agatagaatc tttgagtggc tgtaactggt ttcatggcaa ttgctatcca ccagcagggt
     2521 atggttgggg tgatggaaca atttatagtg gtgatttttg cccatgagga ggcaacagat
     2581 gaaaataaga agcattgcaa cattgattgt aatatttgcc tttttactct c
//
LOCUS       DAOSRI010000235         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_35840_length_2610_cov_4.010959, whole genome shotgun sequence.
ACCESSION   DAOSRI010000235 DAOSRI010000000
VERSION     DAOSRI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35840_length_2610_cov_4.010959"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PK717_06910"
     CDS             complement(<1..1472)
                     /locus_tag="PK717_06910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR47424.1"
                     /translation="MFPLPSLELKTIEEDSFLGRYSIEPLHSGYGVMIGNSLRRVLLS
                     SIPGAAPIAVRIEGVPHEFSVIEGVVEDVVQIILNLKKLVVAMDGDEIRTLTLDVSGE
                     RVVTAADIIPDAGLTIINPELHIAELSSSKSTLRMDILVSKGRGYVPTEKNKRPDYPI
                     GTIWMDAMFTPVKNVKFAVEQTLIEQYLDFEKLTLDILCNGAVPPKEALDNALQILSE
                     YYMAIGNPELLRKKETSLEETLEEKLALLIENVLPGLSPKVQHLLVYADIKTMRDLLM
                     KTKTELVQHHNITHKAITELAKALEESQEYATFSQFNFPFLKEEGKAEIEDEPKEAPV
                     TKAGKVEKEPEIMEPEEAIEEEIVEEKVETPVVEEPKPAKPSKVKAEKEQPKPEPVQI
                     PKEEPKQDITKGFSIQDISLSKSTMTFLQQNKITDLASLLSMSKSQIEGMKGFKPKMI
                     QEIEKNLDKVGLVLAQDQMFGNLSPQEREKAVWSMSIEDLV"
     gene            complement(1482..2114)
                     /gene="rpsD"
                     /locus_tag="PK717_06915"
     CDS             complement(1482..2114)
                     /gene="rpsD"
                     /locus_tag="PK717_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR47425.1"
                     /translation="MGRYIGPVCRLCRREKTKLYLKSFRCYGSKCPLEKSFTIPGMHG
                     PKKKPKEKLSDYNLHLREKQRVKRIYGMFENQFRAAFEEARLQREIPTGDKLLELLER
                     RLDNVVFRSALAPSRSVARQLVSHGHVLINGKKISVPSHTIRQGDEITLDTTAKKIPL
                     VTDSVKAHLSVPNWISLDSNNFQVKITRPPTRADIDTQANVQLIVEFYSK"
     gene            complement(2130..2471)
                     /gene="rpsK"
                     /locus_tag="PK717_06920"
     CDS             complement(2130..2471)
                     /gene="rpsK"
                     /locus_tag="PK717_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR47426.1"
                     /translation="MLTVAGAVNRPATLWVPVGTPIGDVLSWASGGSSGFKGTRKGTA
                     FAAQVAAQTACKKAQDMGMRQAKLFVKGPGSGRETAIRAIGASGIEIITIKDVTPIPH
                     NGCRPPKQRRV"
BASE COUNT          563 a          633 c          659 g          755 t
ORIGIN      
        1 acaaggtcct caatgctcat tgaccatact gccttctccc tttcctgcgg ggagagattg
       61 ccaaacatct ggtcctgggc aagaacaagc cctaccttgt caaggttctt ctcaatctcc
      121 tgaatcatct tgggtttgaa tcccttcatg ccctcgattt gtgattttga catcgacagg
      181 agtgaagcga gatccgtgat cttgttttgc tggaggaaag tcattgtgct cttggaaagc
      241 gaaatatcct gaatcgaaaa accctttgtg atatcttgtt ttggttcctc ttttggaatc
      301 tggaccggct ctggttttgg ttgttccttc tcagctttga cctttgaagg ttttgctggt
      361 tttggctcct caacgacagg ggtctcaacc ttttcttcca ctatctcttc ttcaatagcc
      421 tcttcaggct ccatgatttc tggttctttt tctaccttgc ctgcttttgt tactggcgct
      481 tcttttggct catcttctat ttcagcctta ccttcctctt tcaagaatgg gaagttgaac
      541 tgggagaagg ttgcatactc ctgagattct tctaaggcct ttgcaagctc tgtgattgcc
      601 ttgtgggtga tgttgtggtg ctggacgagt tcggtcttgg ttttcatgag cagatcgcgc
      661 attgtcttga tgtctgcgta gacaagaagg tgctggacct ttggtgaaag acctgggagc
      721 acattttcaa tgagcagggc aagtttttcc tcaagggttt cctcgaggga ggtttctttc
      781 ttgcggagaa gctccggatt gccaattgcc atgtagtatt cggaaagaat ctgcagagcg
      841 ttgtcgaggg cttcttttgg cggtacggca ccattgcaaa ggatgtctaa ggtcaatttt
      901 tcgaaatcaa gatactgttc aataagcgtc tgctcgacag cgaacttgac gtttttgacc
      961 ggtgtaaaca tagcatccat ccagattgtg ccaatgggat aatcggggcg cttgttcttt
     1021 tctgtcggta cgtagcctct gcccttagat acaaggatat ccatccttaa ggtcgacttc
     1081 gaagaagaca gctcggcaat gtgcaattct ggattgatga tggtgagtcc ggcatcaggg
     1141 atgatgtcgg cagcagtaac tactctttct ccagatacat cgagagtaag cgtccttatc
     1201 tcatcaccat ccattgccac aacaagtttt ttgaggttaa ggatgatttg aacaacgtcc
     1261 tcaacgacac cctcaatgac tgagaactcg tgcgggacgc cttcaatacg gactgcaatc
     1321 ggtgcagcgc ccggaatgga cgacagaagg actctcctca agctgttgcc tatcattaca
     1381 ccatagcccg aatggagcgg ctctatgcta taacgaccga gaaaagaatc ttcttcaata
     1441 gtcttcagtt caagacttgg aagtggaaac actgtccccc cttacttaga gtagaactcg
     1501 actatcaact ggacgtttgc ctgggtgtcg atatctgctc tggtcggggg tcttgtgatc
     1561 ttgacctgga agttgtttga atcaagcgag atccagttcg gtactgacaa atgtgccttc
     1621 acgctgtccg taacaagcgg aatctttttt gccgttgtat cgagagtgat ttcatcgccc
     1681 tggcgaattg tgtgcgaagg tacgcttatc ttttttccat taatgagcac atgcccgtgt
     1741 gagacaagct gtcttgctac tgagcgggat ggcgccagcg ctgatctgaa tacaacattg
     1801 tccaatcttc tctcaaggag ctcaagaagc ttgtcaccag tcgggatttc acgctgtaat
     1861 cttgcttctt caaatgcggc gcggaattgg ttttcgaaca taccataaat cctcttcaca
     1921 cgctgttttt ccctgaggtg gaggttgtaa tcagaaagtt tttctttcgg ttttttcttt
     1981 ggaccatgca tgcccggaat cgtaaaagat ttctctaggg ggcactttga gccataacat
     2041 ctaaatgatt tcaggtagag ttttgttttc tctctcctgc aaagcctgca aaccggacct
     2101 atatatctac ccatttggtt ctcctaaact tagactctgc gctgttttgg cggacggcaa
     2161 ccattgtgcg gtattggggt aacgtctttg atggttatga tttcaatacc cgatgcaccg
     2221 attgctctga tggcggtttc cctgcccgag ccagggccct taacaaaaag cttggcctgt
     2281 ctcataccca tatcctgtgc cttcttgcag gcggtctggg cagcaacctg ggcagcaaaa
     2341 gcggtacctt ttcttgtgcc tttgaaaccc gatgacccac ctgatgccca ggaaaggacg
     2401 tctccgatgg gggtgcccac gggcacccac agcgtggcgg gccggttgac ggccccggcg
     2461 acggtcagca gccggtgggt gaccggcagc ccatcggcgg cgcggaccac gttgaacagc
     2521 gtctggacgt tgaggacgag gcagccgacg tgcaggggca ggccggtctc gggcacgagc
     2581 cggccggtgg tctcgtagac gaggacaaac
//
LOCUS       DAOSRI010000236         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_36262_length_2586_cov_3.599368, whole genome shotgun sequence.
ACCESSION   DAOSRI010000236 DAOSRI010000000
VERSION     DAOSRI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36262_length_2586_cov_3.599368"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2396)
                     /locus_tag="PK717_06925"
     CDS             complement(96..2396)
                     /locus_tag="PK717_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR47427.1"
                     /translation="MMVQQVLVVGISIVFLALLWIFFAPKKIRSSDREKGGEVHTKLI
                     ITGMHCTGCALNIENTLKDLEGTKNIGVNFSTGLADVAYDPSKVAIGDIVKSVSGIGY
                     GARLATDQASALKQARLAKESDVRKIRNNLLIGLPFAIYVTVGTMLMMFYHFPMWFVW
                     TLVAAAVPVMFWTGWHFFISAWKIALHRQTDMNTLVALGVLASFIYSIVAIIYPGIGE
                     HQLYLDGSVVIVMLVLLGNYLRGRAELSAMKEIFDVASLLPEKAHRIKDGVVEDVPIS
                     ELAAGDIVQIKATERAPADCTIVKGSSEFNNAHITGESMPVPVSVGDSVYAGAINGFG
                     LVEAKCDKVGESTVLSRVIELIGAAIQTKPKIQGLVDRIASIFVPTSITISITAFVVW
                     FLLGFTNIGINSAISVLVISCPCALGLATPTSISIALGISAKSGMLIKNARSLEALEE
                     VKAFVFDKTGTLTAGRPEVTSIHPELVSEDELIKLTAMAESGSEHTLAQAIKNLAISR
                     NIPIETPQNFEAVPGLGVKATFAGGELVVGGPRMVEKLGLKIPLVLLGTFRPEEAAFY
                     VSLDNKIIGGFGLSDPLRPEAFQAMKELIELGIEPVLVSGDRKEVCESVASQLGIKRV
                     YSQTLPEEKVQIVEALRADGFVAMMGDGVNDAAALAMADASFAPRQGTGMALDTADVT
                     IMNENLLLVPQTVRLARATKKNIQWNLFWAFIYNTLAIPVAAGVLYPVGIQLNPMIAS
                     AAMAASSITVVMNALRLKLFKITIVK"
BASE COUNT          713 a          644 c          573 g          656 t
ORIGIN      
        1 attcgagata atagcagaac ctgtagacaa aaaacctgag ttctgatcaa gattttcatg
       61 gaagtcaaaa aagccccaag aaagcggggc tttttttatt ttacaattgt aattttgaac
      121 agttttagtc ttagcgcatt catgacaaca gttattgatg aagcggccat ggccgccgag
      181 gcaatcatgg gatttagttg tatcccgact ggatagagaa cgcctgctgc aacaggaatg
      241 gcaagtgtgt tgtagataaa ggcccagaag aggttccact ggatgttttt cttcgtcgcc
      301 ctggccagtc tcacagtctg gggaacaagc agtaaattct cgttcattat ggttacatca
      361 gcggtgtcca gcgccatacc ggtgccctgt cttggtgcaa aggaagcatc ggccatcgcc
      421 agcgcagcag catcattgac accatcaccc atcattgcca caaagccatc ggcccttagc
      481 gcttcaacga tctgtacctt ttcctctggg agagtttgcg aataaaccct ctttatacct
      541 aactgggacg ccacagactc acaaacttct ttacggtctc cagaaaccag aacaggctca
      601 atgcccagtt cgatcagctc tttcatggcc tggaaggcct ccggtctcaa gggatcagaa
      661 agtccaaaac caccaattat cttgttatcc agcgacacat aaaatgcggc ttcttcaggc
      721 ctaaatgtac ccaataatac aagtggaatt ttcagcccaa gtttttccac catccttggc
      781 ccgccaacaa ccagctcacc gccggcaaag gttgccttta cgccaagtcc aggcacagcc
      841 tcaaagtttt gcggggtttc tatggggatg ttccttgaaa tggcaagatt ttttatggcc
      901 tgagcaagtg tatgttcgga accagactcg gccatcgctg ttaattttat cagttcatcc
      961 tctgagacca attccgggtg tatggacgtc acttcaggtc taccggctgt cagggtgcct
     1021 gttttgtcaa aaacaaatgc cttgacctct tcaagcgcct ccagcgacct ggcattcttg
     1081 atgagcatcc cggattttgc cgaaataccc aaagctatgg aaattgatgt gggagtggca
     1141 agcccaagcg cacaggggca ggaaatgaca agcacagaaa ttgccgaatt gatgccaatg
     1201 tttgtgaaac caaggagaaa ccacaccaca aaagccgtga ttgaaatcgt tatagaggta
     1261 ggcacaaaaa ttgaggcaat cctgtcaaca agcccctgta ttttcggctt tgtttgtatg
     1321 gcggcaccga tcagctcgat gacccttgaa agaacagtgc tctcgccaac cttatcgcac
     1381 tttgcttcca cgagcccgaa tccgttgatt gctccagcat agacactgtc accaacacta
     1441 accggcacag gcatgctctc accagttatg tgggcattgt tgaattcgga ggaacctttc
     1501 acaatggtgc aatcggcagg tgccctttca gtggctttta tttgtacaat gtcgcctgcc
     1561 gcaagctcag atattggaac atcctccaca acaccatctt tgatcctgtg ggctttttca
     1621 ggcaggagag atgcaacatc aaatatctcc ttcatggccg acaactcggc cctgcctctg
     1681 aggtagtttc caagcaaaac aagcattacg ataacaacag agccatccag atatagctgg
     1741 tgctcaccaa ttccaggata gataatggca actatcgaat aaataaaaga ggcaagcact
     1801 ccaagtgcaa caagggtgtt catgtctgtc tgcctgtgga gggcaatctt ccaggcagaa
     1861 atgaagaaat gccagccggt ccagaacatt acagggacag cggcagcaac aagtgtccag
     1921 acaaaccaca ttgggaaatg gtagaacatc atgagcatgg ttccaactgt gacatagatg
     1981 gcgaacggaa gcccgatgag aaggttattt ctgatctttc ttacatcaga ttcttttgcc
     2041 agccttgcct gcttgagggc ggaggcctga tcggtggcca accttgcacc atatccaata
     2101 ccagagactg atttgactat gtcgccaatt gcaaccttcg atgggtcata ggcaacatca
     2161 gcaagcccgg ttgaaaaatt gaccccgatg ttttttgtac cctcaaggtc cttgagggtg
     2221 ttttcgatat tcagggcgca accagtgcaa tgcatccctg ttattatgag tttggtgtgt
     2281 acctctcccc ctttttcccg gtcactggaa cgaatttttt tgggagcaaa aaatatccat
     2341 agcaatgcga ggaagacaat tgaaataccg acaacaagaa cctgttgcac catcatgacc
     2401 tccttgcgat attttacaat gtaactttca aaccacaatg gcccaacaaa atcataatcc
     2461 agcaattgtg aattttttcc aaatataaaa acaacatttt agctaatggg ccaatcccgc
     2521 ttgtttaata tgcacgattg gcccgatttc ttgacgtgcc ccaaggttgg ggatattcat
     2581 tcttat
//
LOCUS       DAOSRI010000237         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_36436_length_2577_cov_3.760508, whole genome shotgun sequence.
ACCESSION   DAOSRI010000237 DAOSRI010000000
VERSION     DAOSRI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36436_length_2577_cov_3.760508"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..2277
                     /locus_tag="PK717_06930"
     CDS             292..2277
                     /locus_tag="PK717_06930"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOR47428.1"
                     /translation="MVTMNANPDDLFQELVEKTKYLGDVKGKLLEAYQTASKYHAGQK
                     RESGEDYITHPLSVANILAELETDLPTLQAAILHDTLEDTPLKEEELKEKFGLEVLSI
                     VQGVTKLSKVQFRSSKEQQLENTRKMLLAMASDIRVVLIKLADRLHNMRSVEVFRKDK
                     QKRIATDTMEIFVPLASRLGIYRIKWELEDLSFRYLEPEIYYDLSKQVALKRSEREKL
                     VQQVMEELSEYLQNNDIKCEISGRAKNLYSIYKKMEAEKKGLDEVFDLHAVRVLVDDI
                     STCYKVLGIVHSIWKPLPGRIKDYIAVPKSNGYQSLHTTVIGPWGNPVEIQIRTHQMH
                     DDAEYGIAAHWRYREGKIQFSPDYNQKLSWLRQLLEWQREIKPSDEFVEMVKTDLFSD
                     EVLVFTPKGDIINLPVGATPVDFAYRIHTNVGHSCIGAKVNGQIVQLSYKLQTGDRVQ
                     ILTSKHINGPSRDWLKFVVAHSTKERIRSWFRKQTEEEQPKQKDEPEPVKALPKPRKL
                     PKFGKGSPMISVKGIDNAPLMLARCCNPIYGDPIVGYITRGRGISIHRVNCQNLAQLK
                     KDSERILEAEWIKSDLVRAYVVNISTTVENKPGVLAKLSSVISEEGVNIQMAKATPVD
                     TQRSRVMFAVEVTSIKELQDLISRLERVPEVKKVVRH"
     gene            2274..>2577
                     /gene="dtd"
                     /locus_tag="PK717_06935"
     CDS             2274..>2577
                     /gene="dtd"
                     /locus_tag="PK717_06935"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR47429.1"
                     /translation="MRAVVQRVGRASVSVDGKIVGQIESGLLVFVGVGQGDTQQDADY
                     IATKILGMRIFQDDAGKMNLDVTQIGGSVLIVSQFTLFGDVRKGKRPDFTMAALPED"
BASE COUNT          790 a          509 c          625 g          653 t
ORIGIN      
        1 gattaggatt gatacaggtt tcagcctttt catggcatac ctccaataaa ttaattcaac
       61 aaatacattt cagactctat cagtttatgt taacaaatta ttttttcaag ccgtattttc
      121 tttattgaga aatggtttat tttgattttg gaaaagttaa atatcatggt ttgtaaatat
      181 gacaatatca gtgaaaacgt cgatttttag caaaattgtg tattttttgc gcaatcaatt
      241 caatctttat gtgttgacct gagcagtcac atgcaaatat aatctccccc aatggttacc
      301 atgaatgcca atcctgacga tctattccag gagttggttg aaaagacgaa atatcttggt
      361 gatgtcaaag ggaaacttct agaggcctac cagacagcat caaagtatca tgctggccaa
      421 aaaagggaga gcggcgagga ctatataaca caccccttga gtgtggccaa tattcttgct
      481 gagctggaaa cagaccttcc aacgctgcaa gctgcaattc tgcatgacac gcttgaagat
      541 acacccctca aagaagaaga gctaaaggaa aaatttggcc tggaggttct ttctattgtc
      601 cagggagtga caaagctgtc aaaggtacag tttaggtcat ccaaggagca gcagcttgaa
      661 aacaccagaa aaatgcttct tgcgatggca tcagacatca gggtggtttt gataaaactt
      721 gctgacaggc tccataatat gcggtctgtg gaggttttta gaaaagacaa acaaaaaaga
      781 atcgcaacag acacaatgga gatttttgtg cctctggcat ccaggcttgg aatatacagg
      841 ataaagtggg agcttgaaga cctgagcttc cgctatcttg agccggagat ttattacgat
      901 ttgagcaaac aggtggcact gaaaagatct gagagggaaa agcttgtcca gcaggtcatg
      961 gaggagcttt ccgaatacct gcagaacaac gacataaaat gcgagatttc tggcagggca
     1021 aagaaccttt attcgatcta caaaaaaatg gaggccgaga aaaagggact tgacgaagtc
     1081 tttgatctgc atgcagttag ggtgttggtc gatgatattt ctacttgtta taaagtcctt
     1141 ggtatcgtcc attcaatatg gaagccattg cctgggagaa taaaggacta catcgcggtg
     1201 ccaaaatcca atggttacca atccttgcac accacagtta ttggtccgtg gggtaatcct
     1261 gtcgagatac agattaggac gcaccagatg catgatgatg ctgaatatgg cattgcagcc
     1321 cactggcgtt acagggaagg gaaaatccag ttctctccag attataacca gaaactttcc
     1381 tggctccgcc agctccttga atggcaaaga gaaatcaagc cttcagacga gtttgtggag
     1441 atggtcaaaa cagacctctt ctcagatgaa gtccttgttt tcactcccaa aggcgacatc
     1501 atcaaccttc cagttggcgc tacaccggtg gattttgcat atcgaatcca caccaatgtc
     1561 ggccatagct gtattggcgc aaaagtcaac ggtcagattg tccagctttc atacaagctg
     1621 cagactggtg acagggttca gatactcaca agcaagcaca taaacggtcc ttccagggac
     1681 tggctcaaat ttgttgttgc acattctaca aaggaaagaa tcagatcatg gtttaggaaa
     1741 cagaccgaag aagaacagcc aaaacaaaag gatgagccgg aaccagtaaa agcattgcca
     1801 aagccaagaa agctccccaa atttgggaag ggttcaccaa tgatttcggt aaagggaata
     1861 gacaacgcac ctttgatgct cgcaaggtgt tgcaacccga tatatggtga cccgattgtt
     1921 ggctacataa caagggggag aggtataagt attcacaggg tcaattgtca aaatttggcc
     1981 caactcaaaa aggacagtga aaggatactt gaggctgagt ggataaagag tgatttggtc
     2041 agggcctatg tcgtcaatat ctcgacaact gttgaaaaca aacctggtgt tttggcaaaa
     2101 ctctcaagcg tcatctcgga agagggcgta aacatccaga tggcaaaagc aacacctgtg
     2161 gacacacaaa ggtcgcgtgt catgttcgcc gttgaagtga catccatcaa ggaactccag
     2221 gacctcatct ccagactcga aagagtgcct gaggtaaaga aggtggtgag gcattgaggg
     2281 ctgttgtgca gagggttggc agggcatcag tgtctgtgga tggcaaaatt gttggccaaa
     2341 ttgaatctgg attgcttgtt tttgttggtg ttggccaggg ggacacccag caggatgctg
     2401 actatatcgc aacaaaaatc cttggaatgc gaatatttca ggatgatgca ggaaagatga
     2461 atttggatgt aacccagatt ggtggatcgg tgcttattgt ttctcaattt acactgtttg
     2521 gcgatgtgag aaagggcaag aggcctgatt tcacaatggc tgctttgcca gaagatg
//
LOCUS       DAOSRI010000238         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_36482_length_2575_cov_4.048810, whole genome shotgun sequence.
ACCESSION   DAOSRI010000238 DAOSRI010000000
VERSION     DAOSRI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36482_length_2575_cov_4.048810"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK717_06940"
     CDS             <1..1333
                     /locus_tag="PK717_06940"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR47430.1"
                     /translation="TIPAGSLKQRQKNGLLETSSSKRSIGAGIRVLRNGAWGFASTSD
                     LDPDSLAKALKDAEIAAITIGSQSQKKVPVLSPKRLAKKDYKYKTKRDLEDIPFEEKI
                     ALAIRIESLIRKSSQFIVTATTYYSETTESKWVMTNDGAKCHSRLSKPDFYCIAVASK
                     DSQMQSSFESSGVVGGFDELFDSRTPEQMAEKAARIAVDKLDAPYVEGGMHDVVLKPG
                     MVGILAHEAIGHTVEADFVLSGSITAGKIGQKIASDLITMVDDGPNLMKIGNPAGYIE
                     VDDEGVEAGGTVIIKDGILSSYLHSRETAELFGVEPTGNARAWGYTDVPIIRMRNTYI
                     KPGKNSFDEIIGSVDKGYLLVNPENGEADANAEFMFGCGEAYEIAKGKVGRLVKNVTI
                     SGQAFDVLKSVDMVGNDFEFAMGSGACGKIQPAKVDGGGPSIKCKAMLGGR"
     gene            1330..>2575
                     /locus_tag="PK717_06945"
     CDS             1330..>2575
                     /locus_tag="PK717_06945"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR47431.1"
                     /translation="MMDTKEIAERTLGMCKKAGAQNAEVAVSHSKNVSVSAQDNEIHV
                     ATSDKGSWLGVRVINSHSQGFSCVNSFEDSKVQMAIEEAMAIAKSSPPDDSNVLPDPG
                     ETISIGDLHNQSLAEMGLDKVVGYAKQMLLECKTYDSRISVDMAKFNLASTVSTLANT
                     KGINLSQERTGIFYFVYGMAKEGETVSSMDGAFGATTDPQKINVSEVARKFSKNVIES
                     LGAKSGKSFEGPVILFGDALSELILSTVLWSCNANSIQMGESRFAGMQGKQVASEKLS
                     ILDDGRRPGRFESTNFDREGIAPKIMWPIKNGIFTEPFHNARTAARDKTISNGHAAGS
                     PSSTPSVSTTNIIVETGKTTLEELIADTKLGLLVKRYSGNIDPSSGDFSGVVKGGYLI
                     ENGKITTPLLGTMIAGNVFEMIK"
BASE COUNT          762 a          594 c          660 g          559 t
ORIGIN      
        1 cacgattccg gcagggtcgc ttaaacagag acaaaaaaac ggcctgcttg aaacatccag
       61 ctccaaaagg agcattgggg caggcataag agtgctcagg aacggcgcat ggggctttgc
      121 ttcaacatct gacctcgatc cggattctct tgccaaggcc ctgaaagatg ccgagatagc
      181 agctattaca attggctcgc aatcacaaaa gaaagtgcca gtgctatccc caaagaggct
      241 ggccaaaaaa gactacaaat acaaaacaaa aagagacctc gaagacatcc cgtttgaaga
      301 aaaaatagcc ttggccataa ggatcgagtc gctgatccgg aaaagctctc agtttatcgt
      361 tactgctacg acttattact ccgaaacaac agaaagcaaa tgggtaatga caaatgacgg
      421 ggcaaagtgc cacagcaggc tttcaaagcc tgatttttac tgcatcgctg ttgcctcaaa
      481 agatagccag atgcaaagct cgtttgaatc cagtggcgtc gttggcggct ttgatgaact
      541 ctttgattcc aggacgccag agcagatggc cgaaaaagct gccaggatag ccgttgacaa
      601 actagatgca ccttatgtgg aaggcgggat gcacgacgtt gttctaaaac cgggcatggt
      661 tggcatcctt gcgcatgagg caatagggca cacggtcgag gcggattttg tattgtcagg
      721 gtcgataacg gcaggcaaga ttggccagaa aatagcttca gacttaatca cgatggttga
      781 tgacgggcca aatttgatga agattggcaa tcctgcaggc tacatcgagg ttgacgatga
      841 gggtgtagag gccgggggaa cggtcatcat aaaagacggc attttgtctt catatctcca
      901 cagcagggaa accgccgagt tgttcggtgt agaacccact ggcaatgcaa gggcatgggg
      961 ctacaccgat gtcccgataa tacgaatgag aaacacctac ataaaacctg gcaaaaacag
     1021 ttttgatgag atcattggct cggtagacaa gggctacctt ctggtcaacc ctgaaaacgg
     1081 cgaagcagac gcaaacgcag aattcatgtt cggatgcggt gaggcctacg aaatagccaa
     1141 aggaaaggtc ggcaggcttg tgaagaacgt taccatctcc ggccaggctt ttgatgtttt
     1201 gaaaagtgtg gacatggtcg gaaacgactt tgagtttgca atgggttccg gtgcttgtgg
     1261 gaaaatacag cctgccaaag tcgatggcgg cgggccctcg atcaaatgca aggcaatgct
     1321 tggaggcaga tgatggacac gaaagaaata gcagaacgca cgcttggaat gtgcaaaaaa
     1381 gccggtgcgc aaaatgccga ggtggccgtt tcccacagca aaaatgtctc tgtcagtgcc
     1441 caggacaacg agatacacgt cgcgacttcg gacaaaggca gctggcttgg cgtaagggtc
     1501 atcaattccc attcacaggg cttttcatgc gtaaattcct tcgaagactc aaaagtgcag
     1561 atggccatcg aggaggcaat ggcaattgcc aaatcctctc cacctgacga ttcgaatgtc
     1621 cttccggacc ccggagaaac aatctcaatt ggcgacctcc acaatcagag ccttgctgaa
     1681 atgggtcttg acaaggttgt tggctacgcc aaacagatgc tcctggaatg caaaacctac
     1741 gacagcagga tttcagttga catggccaaa ttcaacctgg cttccactgt ttcaacgctt
     1801 gcaaacacaa aagggataaa tctttcccag gagaggaccg ggattttcta ttttgtctat
     1861 ggcatggcaa aggaaggtga aacagtctct tcaatggatg gtgcctttgg tgccacaacc
     1921 gaccctcaaa agatcaatgt ttcagaagtg gcaaggaaat tctcaaaaaa cgtcatagag
     1981 tcacttggcg caaagagcgg aaagagcttc gaaggcccgg taatcctctt tggggatgca
     2041 ctaagcgagc ttatactttc aactgtcttg tggtcttgca atgccaattc catccagatg
     2101 ggcgagtcaa ggtttgccgg aatgcagggg aaacaggttg cttctgaaaa actgtccatt
     2161 cttgatgatg gaaggcgccc tggcaggttt gaatcaacaa actttgacag ggaaggcatc
     2221 gcaccaaaga tcatgtggcc aataaaaaat ggcattttca cagagccttt ccacaacgca
     2281 aggacggcgg cgagggacaa aaccatatca aatggccatg ccgcaggatc accctcctca
     2341 actccctccg tctctacaac caacataatt gtggaaactg gcaaaaccac gctcgaagag
     2401 ctcatcgcag acacaaaact tggccttctt gtgaaaagat attctggaaa cattgatcct
     2461 tcttccggtg atttctctgg ggttgtaaag ggtggctacc tgatcgaaaa tggaaagata
     2521 acaacacctc tccttggcac aatgattgct ggaaatgtat tcgagatgat caaaa
//
LOCUS       DAOSRI010000239         2559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_36798_length_2559_cov_5.470048, whole genome shotgun sequence.
ACCESSION   DAOSRI010000239 DAOSRI010000000
VERSION     DAOSRI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36798_length_2559_cov_5.470048"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(441..1787)
                     /locus_tag="PK717_06950"
     CDS             complement(441..1787)
                     /locus_tag="PK717_06950"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOR47432.1"
                     /translation="MIIPKWSKSSWRSFPIQQQPHWPDANVYEDVLDKLSQLPALVFA
                     GETRALKNQLAEVCEGNAIILQAGDCSEDFSRCHGPRIHDLLKVILKMSVILAYAGEK
                     KIVKIGRMAGQYAKPRSSDTEVVNGREIPSYRGDMVNSSDPTPEARMPDPRRILDGYF
                     RAAATLNLVRAFTKGGYAALDKIQSWSDASFGSFPASRQYDELVNGIRKAMNFVAAMG
                     IDTKAPQLNEITVYTSHEALLLGYEEAMTRVDTTTGKWYDTSAHMLWIGDRTRQLDGA
                     HIEFLRGVCNPIGVKIGPNHVVENVKRFVERLNPENEPGRLTLITRFGTDKIGTLLPS
                     LVRGLKSEGFKLVWCCDPMHGNTYTNTFSRKTRKCEDILGEIRSFWEIHHAEGTMAGG
                     IHLELTGDNVTECTGGNRQLLDSDLHLNYQSNCDPRLNAEQGIDIAFEFAKILRSN"
     gene            complement(1927..>2559)
                     /locus_tag="PK717_06955"
     CDS             complement(1927..>2559)
                     /locus_tag="PK717_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR47433.1"
                     /translation="YIRVDMHGKKPVPQSWRLSDGTVPMIHLDLAVHLHHLIHYLIEQ
                     KPIEVVADQDSYGWFAEVIDNVGCLCRYSDGVKGQIWFSKSALGHRNGLRLRIYGTKA
                     SAEWFQACPEELVVCHADGRRVIIDRAASVEVANLQRYNRFKAGHPAGFIEAFANLYS
                     DIADCLQQFKKTGQWQSDNVFSAELALEGLQIVEAMVKSTDTKTWQSVCS"
BASE COUNT          682 a          629 c          512 g          736 t
ORIGIN      
        1 tcgccaccgg cccgacccag catcctcatc atatggaaga ctcttaaatt ggctcactgt
       61 ggacatagtc gtagccaacc aaaccaaaac cgtggtacaa gagactcgtg gacatgctcc
      121 cccttttgta accggaaatc cttagcaaat ttccttttgg ggggtatgtc catttttttc
      181 aactacaaaa agtacgcttt agtcggatga accttgaatg ccatgtcggt ctccttcaaa
      241 catgaatttg aaggaaaact accagaaata tcaattattg tcaagtaata tcagcatgtt
      301 aaatgattgt taatcgtaaa attacccgac tttaccaacg ggacttgtgc aaagatttca
      361 cgacatctat atgttttcca ttattcatta tacaatctca ttatcatcgc cgataataag
      421 atcacatgtt gtcgtgaatt tcaatttgat cgtaatattt tggcaaactc gaaagcaatg
      481 tcgattccct gctcagcatt cagacggggg tcgcagttag attggtaatt tagatgaaga
      541 tcactgtcga ggagttggcg gttaccgccg gtgcattctg taacattatc accggttaac
      601 tctaaatgga tacccccggc catagttcct tccgcatgat gaatttccca aaatgaacga
      661 atttcaccaa gaatatcttc acattttctg gttttccgtg aaaatgtatt agtataggta
      721 ttgccatgca tcggatcgca acaccatacg agcttgaatc cctcgctttt taatcctcga
      781 accaacgaag gaagaagcgt gccgatttta tcggtgccaa aacgcgtaat aagggtgagg
      841 cgacccggct cattttctgg gttaagtctt tctacaaagc gtttgacatt ttctacgacg
      901 tgattcggac ctatttttac gccaatcgga ttgcagacac cgcgcaaaaa ctcaatgtgc
      961 gccccatcaa gttgtcgtgt ccgatcgcca atccacaaca tatgggcact ggtatcatac
     1021 catttcccag ttgttgtatc tacccttgtc atggcctctt catatccaag aagtagggct
     1081 tcatgggaag tatatacagt gatctcattt agctggggag ccttggtatc aatccccatg
     1141 gctgcaacaa aattcatagc ttttctaatt ccgtttacaa gttcatcgta ttgacggctt
     1201 gctgggaatg aaccaaaact ggcatcactc catgactgga ttttgtcaag tgcggcgtaa
     1261 cctcctttcg tgaatgccct cacaagattt agggtggcag cagcacgaaa atatccatct
     1321 aaaatccttc ttggatcggg cattctcgcc tcaggtgtag gatcagaact gttcaccata
     1381 tctccccgat aacttggaat ctccctgcca ttaactactt cagtatcaga tgatcgaggt
     1441 ttcgcatatt gaccggccat tcttccgatt ttgacgatct tcttttctcc tgcatatgcc
     1501 aaaattactg acattttaag aatgactttc agaagatcat gtattcgcgg gccatggcat
     1561 cgtgagaaat cttccgagca gtcacctgcc tgcaatataa tcgcgttgcc ttcacagact
     1621 tctgctaatt ggttcttcaa tgctctcgtt tcaccagcaa aaaccaacgc cggcaactgt
     1681 gacagtttat ccaatacatc ctcgtaaaca ttggcatcag gccagtgtgg ctgttgttgg
     1741 ataggaaaag aacgccagct tgattttgac catttgggaa ttatcatttt aaaacctcgc
     1801 tacactgaca agtttccggg attcaatatg ggcaccaacg accgctaatc atacattatc
     1861 ctatcattcc tctttaagtg tctcataatg cagaacctat ctctgtggga caaagttcat
     1921 tttacactaa ctgcaaaccg attgccatgt cttcgtatct gttgatttaa ccatggcttc
     1981 gacaatttga agcccctcca gagccaactc ggcgctaaat acattgtccg attgccattg
     2041 cccggtcttt ttaaattgtt gtagacaatc ggcaatatcg ctgtaaagat ttgcaaatgc
     2101 ttcgataaag ccggccggat ggccggcttt gaatctgtta taccgctgca agtttgccac
     2161 ttcaactgat gcggcgcgat caattatcac acgtcgtcca tccgcatggc atacgacgag
     2221 ttcctccgga caggcctgaa accactctgc agaagctttt gtgccgtaaa ttcgcagacg
     2281 cagaccattc ctgtggccga gcgccgattt gctgaaccat atttgtcctt ttaccccatc
     2341 agaataccgg cagaggcacc caacattatc aatcacttca gcaaaccaac cataactgtc
     2401 ttgatcggcc acaacttcaa tgggtttttg ctctatgaga tagtgaatga gatggtggag
     2461 atgcacagcc aggtcgagat ggatcattgg cacggttcca tcggatagtc gccacgactg
     2521 tggcacgggc ttctttccat gcatatccac tctgatata
//
LOCUS       DAOSRI010000240         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_36917_length_2553_cov_2.813451, whole genome shotgun sequence.
ACCESSION   DAOSRI010000240 DAOSRI010000000
VERSION     DAOSRI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36917_length_2553_cov_2.813451"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..979)
                     /locus_tag="PK717_06960"
     CDS             complement(128..979)
                     /locus_tag="PK717_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOR47434.1"
                     /translation="MKKIAFILVFVMLVPLCASCGKTIADENNCWFEIQHLTSNPTLL
                     SPPCEVQFNAGSSAKPKSWDWKFSDGGTATGQNPKHTFEKEGSYSATMTATYEDGSNA
                     TLMMTGIDIYKGLVQYKRDDFSLEYRIIKPDKNETISSPYIKFSISYPTNGWVGMMII
                     PLDQKNIIDKVQPLISGIDFYGGLKDDKSKETVVTDGYFTNKSLGSDIDKGGKNSILT
                     KDSFEIFGTNKVTVLDFSRLLDTGDKCDVKLEKGETYRIILMASKDLETNLYSNPFKS
                     TELVVKL"
     gene            complement(1164..>2553)
                     /locus_tag="PK717_06965"
     CDS             complement(1164..>2553)
                     /locus_tag="PK717_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOR47435.1"
                     /translation="VTLRPEWHHENDEKGSIKIVMTGSATDPSEWQPHIRNKQGREAL
                     AKRFKDPRDQLKIVIVRDMWLTGFDVPCLHTMYIDKPMSGHNLMQAIARVNRVFKDKP
                     GGLIVDYLGLADNLRKALANYTESGGKGKTAIDQEEAVAIMQSKYEICRDLFHGFDWS
                     SWTTNSAQKINLLPNAQEHILAKNDGKDRLVKAVTELSQAFALAVPNEKALKICDDIA
                     FFQAVRTSLSKSSALNSKPEENLDHAIRQLVSKVVASDQIVDIFTTAGLKKPDVSILS
                     DEFLSEVRNIPQKNLAVELLRKLLDEEIKTRARKNLVQARSFAEVLERSIRAYQNRGI
                     ETAQVIEELIKLAKDIREANKRGENLGLSEEEVAFYDALETNDSAVKILGDNALRTIA
                     RELLETIRRNVTIDWTIKESARAKLRVMVKHILRKHGYPPDKQEKATQTVLEQAELLC
                     KDWTGPDTDILK"
BASE COUNT          627 a          553 c          505 g          868 t
ORIGIN      
        1 ctttgatctc tctgacaagt ttttgcgcag ggactatggc atcctctggc agggatatta
       61 aggaataacg tttataacaa aaagccgaac agcagatgct gttcggcttt ttgttatcag
      121 atgctcatca aagtttgact acaagttctg tagatttgaa agggttagag taaagatttg
      181 tctccaaatc tttagatgcc attagaataa ttctgtacgt ttcacccttt tccaacttca
      241 catcgcattt gtcgccagta tccagaagtc tggaaaaatc caatacagta actttgtttg
      301 taccaaatat ctcaaatgaa tcttttgtta aaatggagtt tttcccacct ttatctatgt
      361 ctgatcccaa tgatttattt gtgaaatacc catcggtgac cactgtttct ttgcttttat
      421 catctttaag accgccgtaa aagtctatac ctgatattaa aggttgcact ttatctataa
      481 tatttttttg atcaagtgga attatcatca tgccaaccca cccatttgtc gggtagctta
      541 tcgaaaactt gatgtatggg gacgagatgg tttcgttttt gtccggtttt atgattcgat
      601 attcaagcga gaaatcatct cttttgtact ggaccagccc tttgtatatg tcgatgccag
      661 tcatcatcag tgttgcattc gagccatcct cataggtggc agtcattgtt gccgagtatg
      721 aaccctcttt ttcaaacgtg tgctttgggt tctggccggt tgcagtgcca ccgtcgctga
      781 atttccagtc ccaggacttt ggttttgcac ttgagccggc attgaactgc acctcgcaag
      841 gtgggctcaa aagtgtcggg tttgacgtaa gatgttgtat ttcaaaccag cagttgtttt
      901 catctgcaat tgttttcccg catgaagcac aaagtggcac tagcattaca aaaacaagaa
      961 taaaagctat ttttttcata gcatacccct ctccgttttt atgtttgctc ttccagcctg
     1021 acatttcacc cgattatccc tagtgtgttt tgggtgtgat tgttccggcc aaatgttgcc
     1081 aaattgtcaa cggatttctt ttgccagttc aaacagcact gtcataagcg ttcttgtcct
     1141 cacgtcgtcc cgcgagctgt tcattatttc aaaatgtcag tatctggtcc agtccaatct
     1201 ttgcaaagta attctgcttg ttctaggacc gtttgtgtgg ccttttcttg tttatctggt
     1261 gggtaaccat gtttgcgaag aatatgcttc accataactc tgagtttggc acgagcactt
     1321 tctttgattg tccagtcgat ggtaacatta cggcggatag tctctagaag ctccctggcg
     1381 attgttctta aagcgttatc acctagtatt ttgacagcac tatcattagt ctccaaagca
     1441 tcataaaaag ccacctcttc ttctgaaagg cccagatttt cccctctttt attggcttcc
     1501 ctgatatctt tggctaattt gataagctct tcaataactt gcgcggtttc gatgccccta
     1561 ttctgatagg ctcttataga tctctcaagt acttcagcaa aagatcttgc ttgtacaagg
     1621 tttttgcgcg cacgtgtttt aatttcttca tccagtaatt ttcgtaataa ctctactgcc
     1681 agatttttct gtgggatatt tcgaacctcg gacaagaact catcagataa gattgaaaca
     1741 tccggttttt taagccctgc ggttgtgaaa atatcaacta tttggtcaga tgcaacaact
     1801 tttgatacga gttgtctaat tgcatgatca aggttttctt ctggtttaga atttaatgca
     1861 cttgattttg acaaagaagt gcgaactgct tgaaagaaag ctatatcatc acagattttt
     1921 aaggcttttt cattaggaac cgccagtgca aatgcttgag aaagttctgt aacagcttta
     1981 actagacgat ctttaccatc attctttgct agaatatgct cttgagcatt tggcagcagg
     2041 ttgatttttt gagcagagtt tgtcgtccaa ctggaccagt caaacccatg aaaaaggtca
     2101 cgacaaattt catatttact ctgcattatt gcaacggctt cttcttgatc tatggcagtt
     2161 tttcccttgc caccactttc tgtgtaattg gcaagcgcct tgcggagatt atcggcaagt
     2221 ccaagatagt ctacaatcaa gccgccaggt ttatccttga atacacggtt cactcgagca
     2281 attgcttgca tgaggttatg cccactcatc ggtttgtcga tatacatcgt gtgaaggcat
     2341 ggcacatcaa atcctgtaag ccacatgtca cgcacaatca ctatcttgag ctggtcacga
     2401 ggatctttga aacgttttgc gagtgcctct ctcccttgct tgtttcttat gtggggctgc
     2461 cattctgatg ggtctgtggc tgaaccagtc attacgattt taattgaacc cttttcatca
     2521 ttttcgtggt gccattctgg tctcagggtc acg
//
LOCUS       DAOSRI010000241         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37251_length_2536_cov_3.459492, whole genome shotgun sequence.
ACCESSION   DAOSRI010000241 DAOSRI010000000
VERSION     DAOSRI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37251_length_2536_cov_3.459492"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="ybeY"
                     /locus_tag="PK717_06970"
     CDS             <1..454
                     /gene="ybeY"
                     /locus_tag="PK717_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR47436.1"
                     /translation="IQRRFNMDNKCDVAGPLSDPFSSHIEKFVISCCPEVFGERCPVT
                     VSVAIVDDDEIQSLNRGYRDKDEPTDVLSFEDDLIIEDGLSFVGDIIVSLPYAQRDKG
                     DKSILEYVLFLVAHGLLHLSGLDHKTDEERLQMIERGEELVRKYVPKI"
     gene            438..836
                     /locus_tag="PK717_06975"
     CDS             438..836
                     /locus_tag="PK717_06975"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOR47437.1"
                     /translation="MSPRFKRSFYRAFIGLGIGFEDPMLKRHIVSTIGAIACAVYYKL
                     DSVEMAIVLLCCSLVIGLELLNTAFEKIANELKNPRPSLDVAAASVLMVSLFSLAIGL
                     MFFLPKAAASSPWFLLIVMVLTPISWMVIK"
     gene            837..2123
                     /locus_tag="PK717_06980"
     CDS             837..2123
                     /locus_tag="PK717_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR47438.1"
                     /translation="MDNGLNYLYTFLLLLASAFFSAAEVAFLTSDKIKLKEMASEGIS
                     WASGVLKLRLDREKTLTAILLLNNLANNAATVLATVIAQKLSQSAVVWVTPIMTAIIV
                     IFGEMIPKTAGSRKPISIAKFALFPMRFLIIGLTPIIWILQKITEFFLWIMHLQPDSS
                     QSAVVTEDELEAMINISHQEGLIPTEERVMIEKVFEFGDTTVYDVMVPRVDIVSAPVT
                     STVQELLSIVKEYGHSRLPIYRDNPDNVIGILHVKDLITIVASGGKGEFKLDKVLREP
                     YFIPDSKHISDVFREMRAKRVHLGMVVDEFGGISGLITLEDLLEELVGEIQDEYDTDE
                     EPVKKINENTYIIQANLNMEQVNEVLNTDLSNENYDTLAGFMMDRLGHLGKVGEKVET
                     EDFTFIIDQIRKMRILKVKVMDKREKSKGPEIYTTN"
     gene            2161..>2536
                     /locus_tag="PK717_06985"
     CDS             2161..>2536
                     /locus_tag="PK717_06985"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOR47439.1"
                     /translation="MNKTVAEARVFIKELLRERFNKICSVGITPNFVALHVVGELLEG
                     SPIGFGAQNCYFEDQGAFTGEVSPLMLKDLGCKWCLVGHSERRHIFGETDELVRRKTV
                     ALLRHGIRPIVCVGETLEQRNSG"
BASE COUNT          709 a          499 c          612 g          716 t
ORIGIN      
        1 tatccagcgg aggttcaaca tggataacaa gtgtgatgtt gccggccctt tgtctgaccc
       61 tttcagtagt cacatagaaa aatttgtcat ttcctgttgc cctgaggttt ttggggagcg
      121 ttgccctgtt acagtttctg ttgcgatcgt ggatgatgat gaaatccagt cgctaaacag
      181 ggggtacaga gacaaagatg aaccgactga tgttctctca tttgaggacg acctcataat
      241 tgaagatggg ctaagttttg ttggtgatat aatagtttcg cttccctatg cgcaaagaga
      301 caagggcgat aaatcaatcc ttgagtatgt cctcttcctc gttgcccacg gtttgctcca
      361 cctgtcaggt ttggatcaca aaactgacga agaaaggctc cagatgatcg aaagagggga
      421 ggagctcgtc agaaaatatg tccccaagat ttaaaagatc attctatagg gcctttatcg
      481 gtcttggaat cgggtttgaa gacccgatgt taaagagaca cattgtttca accataggag
      541 cgattgcctg tgcagtatat tacaaacttg acagtgttga gatggccata gttctcctgt
      601 gctgttcgct tgttattggc ttggagttgc tcaacactgc ttttgagaag atcgctaatg
      661 aactaaaaaa tccgcggcca tctcttgatg ttgcagcggc ctctgttttg atggtttcgt
      721 tgttttccct tgctattggc ctcatgtttt tcttgcccaa agcggccgct tcttcgccgt
      781 ggtttttgct gattgttatg gttttaactc ctatctcatg gatggtaata aaataaatgg
      841 ataatggttt gaattacttg tacacgttcc ttctgcttct ggcttccgct tttttctcgg
      901 ccgcagaagt tgcttttctc acctcggaca aaatcaagct caaggaaatg gccagtgagg
      961 gcatttcctg ggccagtggc gtgctgaaac tcaggcttga tagggaaaag acactgacag
     1021 ctatcttgtt gttgaacaat cttgcgaaca atgctgccac agtccttgcc actgttatcg
     1081 cgcaaaaact gagccagtct gcggtagtat gggtaacacc catcatgacg gccataatag
     1141 ttatttttgg agaaatgatt ccaaaaacag cggggtctag aaaacccatt tctattgcaa
     1201 aatttgctct tttccccatg cgatttttga taattgggct aaccccgatc atttggatat
     1261 tacaaaaaat aacagaattc tttctctgga ttatgcatct ccagcccgat tcgtctcaat
     1321 cagccgttgt cacagaagat gaactggaag cgatgataaa tatatcacac caggaaggtc
     1381 tcatccccac cgaagagagg gtgatgatag agaaagtgtt tgaatttggc gacacgacag
     1441 tatatgatgt gatggtgcca agggttgata tagtttcggc ccctgtaaca tccaccgtcc
     1501 aggaactgct ttcaattgta aaagaatatg gacattcaag actaccgatt tacagagaca
     1561 atcctgacaa tgtaataggc attttgcacg taaaagacct tataactatt gttgctagtg
     1621 gtggaaaggg cgaatttaaa cttgataagg tactgagaga accttatttt attcctgaca
     1681 gcaaacacat atctgacgta ttcagggaga tgagggccaa aagggtacac ctcgggatgg
     1741 tcgttgatga atttggagga atctctggac ttataacgct tgaagacctg ctggaagagc
     1801 ttgttggtga aatccaggac gagtatgaca cggatgaaga acctgtcaaa aagattaatg
     1861 aaaatacata cattattcag gccaacctca acatggagca ggtaaacgag gtactaaata
     1921 ctgatctgag caatgaaaat tacgacacac ttgctggttt catgatggac aggcttggtc
     1981 acttgggtaa agtcggtgag aaggttgaaa ctgaggattt cacattcatc attgaccaga
     2041 taagaaagat gcgcatcctg aaagtgaaag tgatggacaa acgcgagaaa tcaaaaggtc
     2101 ccgagattta tacgacaaat tgatggcggt gaaatcatgc ttgtgattgg taattggaaa
     2161 atgaacaaaa ccgtggcgga agccagggtt tttataaaag aactgcttcg cgaacgtttt
     2221 aataaaatat gttctgttgg cattactcca aattttgtcg ccctacatgt tgttggtgaa
     2281 cttctggaag gctcaccgat tggttttggg gcccaaaatt gctactttga agaccaggga
     2341 gcgttcactg gcgaagtttc accattgatg ctaaaagacc tcggctgtaa gtggtgtctt
     2401 gttggtcatt ccgagcggag acatatcttt ggcgagacgg atgaactggt cagaaggaaa
     2461 acagttgctc tcctgaggca tggaatcagg ccaatcgttt gtgttggcga aacactcgag
     2521 cagagaaact ctggta
//
LOCUS       DAOSRI010000242         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37344_length_2531_cov_3.376414, whole genome shotgun sequence.
ACCESSION   DAOSRI010000242 DAOSRI010000000
VERSION     DAOSRI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37344_length_2531_cov_3.376414"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PK717_06990"
     CDS             complement(<1..1275)
                     /locus_tag="PK717_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47440.1"
                     /translation="MDSVWFWVATLLYCAAMIWIGYMIARNYKKKGLSQLEFWIAQRQ
                     LPSWWLAASLTAGWLMLGWIGFGMSQVYMYGATGLWILPIPWFILCIIIILAAPLYRK
                     IGAVSLPQMIEKRFGKSARPLLAVFSFFVFIIWTEAEIFMAGTLLSPFLGIDPKLCML
                     LVAIPVVIYTYLGGFRAVVTTDVYQFSIMAVFMIILAVTAIVSAVNVTGGNVISALVN
                     TATPWAGPGNTFNLGFLGWLFPLILLIGYLPGWLIEQDLSIRMQAAKSTKDARKAAWL
                     GFLLIGVFVIILPAIVAFCSLVVYPPDAAGVNAAIGPNALGIISAFIGKLPVWLAAFM
                     VVGILASQMSTIDTFANVSAMPIAYDIIDPILARKKVADVVRTNMSKIVSAVVIMVAL
                     LLSFISESLNDVYYISSGVLSASIAVQVMFIFW"
     gene            complement(1535..>2531)
                     /locus_tag="PK717_06995"
     CDS             complement(1535..>2531)
                     /locus_tag="PK717_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47441.1"
                     /translation="GNDSFSGRNMNQGAASFGVGNLVNIGGNDSYYCFQVGQAFGFSG
                     GCGVLVDTDGDDKYVGEPGKSNPKDNLINPATGGHDNNRNYSFVQGAGWGRRGDMSDG
                     HGWGGGVGVLVDVTGNDWYECGVYGQATGYWFGTGILNDLEGDDHYEGSFFVQSGTAH
                     MGLTELVDDSGNDTYKVWKAISQGGAHDFSVSWFIDKEGNDNYLCYEEENGQKTSGGV
                     LIGAAITNSLGLHVDMAGDDKYDLVNSSTLGYCLMRTGAVPDNYRYEEWTLGLMIDKG
                     GNDTYTRTLEPNVPDGWPVPANNSVWREVTQPGNPAKSLGFGLDVEKGIVPEAQW"
BASE COUNT          753 a          644 c          511 g          623 t
ORIGIN      
        1 ccagaaaata aacatcacct ggaccgcaat tgaggccgat agcacaccag acgagatgta
       61 atagacatca ttcagggatt cgcttataaa tgaaagtaac aatgcaacca taatgacaac
      121 ggccgataca attttcgaca tatttgtacg aacaacatcg gcaacttttt tcctggcaag
      181 tattggatca atgatgtcat atgctattgg catcgccgaa acatttgcaa acgtatcaat
      241 tgttgacatc tgtgaagcca ggattccaac aaccataaat gccgcgagcc agacagggag
      301 tttaccaatg aaagctgaaa taatgccaag ggcatttggc ccgattgccg catttactcc
      361 agctgcatct gggggataaa caacgagcga gcaaaaagca acaattgctg gaagtatgat
      421 gacaaacact cctatcagaa ggaaaccaag ccaggcagct tttctggcat ctttagtact
      481 ttttgcagcc tgcatcctga tagagaggtc ttgttcaatg agccagcctg gcaaatagcc
      541 aatcaagagt atcagaggga ataaccaccc caaaaacccc agattaaaag tattcccagg
      601 accagcccat ggtgtagctg tattcactaa tgcagaaata acattaccac ctgtaacatt
      661 aacagcagaa acgattgccg tgacagccag gattatcatg aatacagcca taatcgagaa
      721 ctgatacaca tccgtagtta caactgccct gaaccctcca agataagtgt agatcactac
      781 aggtatggct actaaaagca tgcacagttt aggatctata ccaaggaaag gtgaaagaag
      841 ggtacctgcc atgaaaatct ctgcttctgt ccagatgatg aacacaaaaa atgaaaatac
      901 tgcaagtaaa ggtctagcac ttttgccaaa gcgtttttca atcatttgtg ggagactgac
      961 tgcaccaatt ttcctgtaca gtggcgctgc caggataata atgatgcaca aaataaacca
     1021 aggaattgga agtatccaca atcctgttgc gccatacatg taaacctggg acattccaaa
     1081 accaatccat ccaagcatca accatccggc agtcaaggaa gctgcaagcc accatgatgg
     1141 taattgacgt tgggcaatcc aaaactcgag ttgggaaaga cccttctttt tgtaattcct
     1201 agctatcata tagccaatcc atatcatcgc agcacaatac aataaagtag caacccagaa
     1261 ccatactgaa tccattttcc actccccttt ttggccacaa tatatttgat ttgatggcaa
     1321 aattcacaaa tgccacaaaa cctttgtggc aacaagtttt gatagaagta tatcaaatag
     1381 tgcttatttt gacaatatgc gcttatttat tccaattttt taagaaagag agtttattaa
     1441 aaatataaca atatcaataa ttcatatgat aaattaacaa aggccggccc cttcaggccg
     1501 gcctttgttt tgaccataca aaactcagac tatcctacca ctgcgcctct ggtacaatgc
     1561 ccttctcaac atcaaggcca aacccgagag actttgccgg gttgcccggc tgtgtcactt
     1621 cgcgccagac agaattattt gctggaactg gccagccatc tggaacattc ggttcgagtg
     1681 ttcttgtgta ggtgtcgttg ccacccttgt ctatcatgag ccccagcgtc cattcctcat
     1741 acctgtagtt gtccggaaca gcgccggttc tcatcaggca atagccgaga gtcgatgaat
     1801 tgaccagatc gtatttgtca tcaccagcca tgtcaacgtg gagcccaagt gaattcgtga
     1861 ttgcggcgcc gatcaaaacg ccacctgatg ttttctggcc gttttcttcc tcataacaca
     1921 aataattgtc gttgccttcc ttgtctatga accatgagac cgagaaatca tgcgcaccac
     1981 cttgggaaat ggccttccac accttgtagg tgtcatttcc gctgtcatca actaattcgg
     2041 tcaggcccat gtgtgcagtt ccgctctgga caaagaagct accttcataa tgatcatcac
     2101 cctcgaggtc attgaggata ccagtgccaa accagtagcc agttgcctgg ccataaacac
     2161 cgcactcgta ccagtcatta ccagtcacgt caactagaac gcctactcca ccaccccagc
     2221 cgtggccgtc agacatatcg ccccttcttc cccatccagc accctggaca aagctgtagt
     2281 tgcggttgtt gtcgtggcca cctgttgccg gattgatgag attgtctttc gggttggatt
     2341 tgcctggctc gccaacatac ttgtcgtcac catctgtgtc aacaagaacg ccgcacccgc
     2401 cagaaaaacc gaatgcctgg ccaacctgga agcagtagta ggagtcgttt ccaccgatat
     2461 tgacaaggtt tccgacacca aagctggccg cgccctggtt catgttcctt ccggagaaag
     2521 aatcattgcc a
//
LOCUS       DAOSRI010000243         2529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37386_length_2529_cov_3.749798, whole genome shotgun sequence.
ACCESSION   DAOSRI010000243 DAOSRI010000000
VERSION     DAOSRI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37386_length_2529_cov_3.749798"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK717_07000"
     CDS             <1..406
                     /locus_tag="PK717_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47442.1"
                     /translation="GLTVADKNRLDNVLQDFDLVAFRNTPISDLSDGTLQRASLAQTV
                     IRDPKVYLLDEPTAHLDLKYKVKTFAEIKKRLGPQNCAVAVVHDTSLARAFCDWTVFL
                     KDGRIFWQGEPKNITQELLGSLYDLEDNQIIL"
     regulatory      413..533
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            577..1125
                     /locus_tag="PK717_07005"
     CDS             577..1125
                     /locus_tag="PK717_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOR47443.1"
                     /translation="MKRLSVKTIAIIGVLSCLSFLGMLIQIPFWVDPILKFDFSEIFC
                     LLGTFMLGPVAGVLIIAIKDILRHFLVTPDAIGLTANFIALSTFCLVSYAVFKPFSKN
                     KNYLIWFGVALLIGVVARALIMLPTNEVSIRLYGLNPQGWGKFIYYSAPLFNLISGTV
                     SSIIMIPFYSFYNRNYIVAKEE"
     gene            1132..2112
                     /locus_tag="PK717_07010"
     CDS             1132..2112
                     /locus_tag="PK717_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47444.1"
                     /translation="MITLGLEEEVFVTEPLLPTTRSLYYLAKLLWKKPSRNYHLTASN
                     FARGKDLKYGMMSGLEVATDICHSPLEIITQLRGLRAELSKIAMGYIVPLGHLIQIDA
                     PTNVCALQIHIGGPEDIERAYDNIAHFLPAIMLMTANAPFRMGERFGQSYRLAVGYAT
                     GPLTGDRFYRFQDLIISRRLGTIEVRALDPVTCTSRIKYVLDAVYALAQIKDHYSLDL
                     ERYKKLREQAITNGANPEIVQLARDLNKYSNFPVELIQHTQSDTTAKMYEKYGLEETY
                     RHIDSLYRMDGILYKKPSPFVGKFLQPTAGFLGYYAPKFPYVTYKWLKEK"
     gene            2112..2270
                     /locus_tag="PK717_07015"
     CDS             2112..2270
                     /locus_tag="PK717_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47445.1"
                     /translation="MKNVFWIILGVLSVSVVLVVGFVFDLLGWMLYFVFGLVIIGIYW
                     LTTGRKQQ"
BASE COUNT          691 a          561 c          573 g          704 t
ORIGIN      
        1 tggattgacc gtagccgata aaaacaggct ggacaatgtt ttacaagact ttgaccttgt
       61 ggcgttcaga aacaccccta tttcagacct ttccgatggc acactgcaaa gggcatcgct
      121 tgcccaaact gtcatcaggg accccaaggt ttaccttctg gatgaaccta cagcccacct
      181 cgacctgaag tacaaggtca agacatttgc agagataaaa aagaggcttg ggccgcaaaa
      241 ctgcgctgtt gcggtcgtgc atgacacatc actggcaagg gccttttgtg actggacagt
      301 gtttttaaaa gacggcagga ttttctggca gggcgaaccg aaaaacataa cccaggagct
      361 gttggggtcg ctttatgacc ttgaagataa ccaaattatt ttatagaatc cagactgcct
      421 tcggggatgg gtgaaattcc ctatcggcgg taaagcccgc aagcaaggca aggccttgca
      481 gatccggtga aattccgggg ccgacggtaa agtccggatg aaagaaggtc tcttctttcc
      541 gtatattttc cccgacttgg cggaaaggag cactacatga aaaggttgtc tgtaaaaacc
      601 atcgcaatta tcggagtctt gtcatgtctt tcgtttttgg ggatgttgat ccaaattcca
      661 ttctgggtgg atccaattct aaagtttgac ttttccgaga ttttttgtct tcttggaaca
      721 ttcatgcttg ggccggttgc cggtgtcttg attatcgcaa ttaaagatat attgcgtcac
      781 ttccttgtaa cacccgacgc aataggtctt acggcaaatt ttattgcatt gtcaacattc
      841 tgtcttgttt cttatgcggt cttcaagcca ttttcaaaaa acaaaaacta tttgatatgg
      901 tttggtgtcg cattgctcat tggtgttgtt gccagggcat tgatcatgct tccaacaaat
      961 gaggtttcaa taagactgta tggccttaat cctcaaggat ggggcaaatt tatttattat
     1021 agcgcccctt tattcaactt gatctcagga acagtatcat cgataatcat gatacctttt
     1081 tattctttct acaataggaa ctatatcgta gccaaggagg aatagtagca attgattaca
     1141 ctcggccttg aggaagaggt ttttgtaaca gaaccccttc ttccaacgac acgctcccta
     1201 tattacctgg caaaactcct gtggaaaaaa ccctctagaa actatcacct cacggcatcg
     1261 aacttcgcaa ggggaaaaga cctaaaatat ggcatgatga gcggccttga agtggccacc
     1321 gacatctgcc attcaccact ggagatcata acccagcttc gagggctgag ggccgaactg
     1381 tcaaaaatag caatgggata catcgttccg ttggggcatc ttatccagat tgatgcccca
     1441 acgaacgttt gcgcccttca aatccatatc ggcgggccag aggacataga gcgcgcttat
     1501 gacaatatcg cacacttcct gccagccatc atgctcatga ccgcaaacgc cccgttccgg
     1561 atgggggaga ggtttgggca gtcttacaga ttggctgttg gctatgcaac agggccgctc
     1621 acaggcgaca ggttctaccg ttttcaggac ctcatcatca gcaggaggct tggaacaata
     1681 gaggtcaggg cgcttgatcc agtcacgtgc acaagtagaa taaaatatgt tttggacgct
     1741 gtttatgcgc ttgcgcaaat aaaagaccac tactcactgg acctcgagag atacaagaag
     1801 ctcagggaac aggcgataac aaatggcgca aaccccgaga ttgtccagct tgcccgcgat
     1861 ttgaacaaat actccaactt cccggttgag ctgattcagc acacgcaatc agacaccact
     1921 gccaagatgt atgagaaata cgggctagaa gaaacctaca ggcacatcga ttcactctat
     1981 agaatggacg gcatcctcta taaaaaacca tctccgtttg ttggaaaatt tcttcaaccg
     2041 accgcaggtt ttttgggtta ctatgcgccc aaatttccat acgtcacata taaatggcta
     2101 aaggaaaagt aatgaaaaac gtcttttgga taatcctggg agtactctct gtttctgtgg
     2161 ttttggttgt tgggtttgtt tttgatttgc ttgggtggat gctctatttc gtttttggat
     2221 tggtaatcat tggaatttac tggctcacta ctggtagaaa acaacaataa gcaatttttt
     2281 tgtttatcaa taatttatct aattttcaaa tactcacatg tccatgagat attgatttta
     2341 tcaggattaa gtaaccacag aaaagcacag cttctggacg actaggccaa ttgttgctat
     2401 aatcccaagg attctgtttt tgctggaggt gcaagtggtt tactggattc tgggcattat
     2461 tgtggtgctg attggcctaa attttcttta ccagcactgg aaaagaaaca tctggttctt
     2521 cagggaccc
//
LOCUS       DAOSRI010000244         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37629_length_2517_cov_3.813160, whole genome shotgun sequence.
ACCESSION   DAOSRI010000244 DAOSRI010000000
VERSION     DAOSRI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37629_length_2517_cov_3.813160"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PK717_07020"
     CDS             <1..899
                     /locus_tag="PK717_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47446.1"
                     /translation="TILIMSIIFVGNTFADDYVISFRTGSTVAKICDKGYKLDTAPVA
                     EGNTVLLPFRFIADRLGLDITWNDQTRTAVADGQGYKISIATKKNSNDATVVYSDSKT
                     LDGSIKARYVIRNSRLLVDHSTFAKILGLSFTVSGKDVNSPVDPDRIRYRWIDFTLPV
                     DSEDGKTITLSNVLSDSSTKALLVEFWYTACTHCKTQLTHLETLYKKYQNKGLVVLSI
                     STDGPGNEGSRRELLEEIGVTFPTLLDEKAEVYNAWMSPGFPNFQLVVPGNKFVVMRN
                     ENYSEEANAKLEQMVQELCSGN"
     gene            862..1743
                     /locus_tag="PK717_07025"
     CDS             862..1743
                     /locus_tag="PK717_07025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOR47447.1"
                     /translation="MSRWYKSFALAISALLFLGASSPVLIWWRLDFRVVEVGDKKDKL
                     DVGPTLANGEYFFPLRYVAEKTGHKVGWMPTVKTAIIRGQGKNIEITAGSRIYKDMSE
                     KKQLLNAPFIKWERMLVPSEILKDRFGLPITKVDGKNLYMEIDPELLKPRATDFTLPD
                     INGKNVTLSEILADKSTKCVLVNFWSTRCVPCRREMPELVKLYNAYKDRGLVVIGICT
                     DSDFLEDERDLLLNELKVTYTILLDPLAETYYKWGGLNVPNMSLVSKDGVIVFQHDGY
                     SPDTAKNAEEAIKKEIG"
     gene            1993..>2517
                     /locus_tag="PK717_07030"
     CDS             1993..>2517
                     /locus_tag="PK717_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47448.1"
                     /translation="MNNVGDMRKKFKSFIVIFVYFMLFSNVFVFLSTAKASQAERNVP
                     PSTRPVDGEKARFPKEKIDITPAGAGTYKLYQEKNGVFSMVLGLIGAQEKPLSGDKTN
                     IQPNRNLVCPAGGPGLVDNITLENRNGTVTTTVYPTSYFGVQDTNITIPPPLVVETNT
                     YIKYNIDYSRKVKIL"
BASE COUNT          776 a          440 c          567 g          734 t
ORIGIN      
        1 cgactatttt gataatgtct atcatttttg tgggaaatac ctttgctgat gattatgtta
       61 ttagcttcag aactggttcg accgtggcaa aaatatgcga taaggggtat aagcttgata
      121 cggcaccagt ggctgaaggt aacacggtct tattgccatt taggtttatt gctgataggt
      181 tgggtttgga tattacctgg aatgatcaaa caagaactgc agttgccgat gggcagggat
      241 ataaaatttc cattgctaca aagaaaaatt ctaacgatgc tacggttgtt tattccgact
      301 caaaaacatt ggatggaagc atcaaagcaa gatatgtcat taggaattcg aggttactcg
      361 ttgaccactc gacatttgct aagattttgg gactttcatt cactgtttct ggaaaagatg
      421 taaattcccc tgttgatccg gacaggataa ggtatcgttg gattgatttt actcttcctg
      481 tggatagtga agatggcaaa acaataactc tatcgaatgt gctttctgat agcagcacaa
      541 aagcactgtt ggttgagttt tggtatacag catgtacaca ctgtaagact cagctcactc
      601 atttggaaac tctttacaaa aagtatcaga ataaagggct tgtggtgctc agtatttcaa
      661 ctgatggccc cggcaatgaa ggctcaaggc gtgagcttct ggaagagatt ggcgttactt
      721 ttccaacatt gcttgatgaa aaagctgaag tttacaatgc ctggatgtca cctggttttc
      781 caaatttcca gcttgttgtc ccgggcaaca aatttgtggt gatgaggaac gagaactact
      841 ccgaggaggc caatgcaaaa cttgagcaga tggtacaaga gctttgctct ggcaattagt
      901 gcccttctat ttcttggcgc atcctctcca gttttaatct ggtggaggct ggattttcgt
      961 gtggttgagg ttggtgacaa aaaagacaaa cttgatgtag ggccaaccct tgctaatggg
     1021 gagtatttct tcccgctccg gtatgttgcc gagaaaaccg gtcacaaagt tggctggatg
     1081 ccaactgtaa aaaccgcaat cataaggggg caggggaaaa acatcgaaat aacagctggt
     1141 agcaggatct acaaagacat gagtgaaaag aaacagcttt taaatgctcc ttttataaaa
     1201 tgggagagaa tgcttgtgcc atcggagata ttaaaagaca gatttgggct tccgataaca
     1261 aaggttgatg gcaaaaatct ttatatggaa attgacccag aattgctcaa gcccagggca
     1321 accgatttta ccctgccaga tatcaatggg aaaaatgtca ctttgtctga gatacttgct
     1381 gataagtcta cgaaatgcgt tttggtcaat ttttggtcaa caaggtgtgt accatgcaga
     1441 agagagatgc ctgagcttgt gaaactttat aacgcctata aagacagggg gcttgttgta
     1501 atcggcattt gcacggactc ggattttctt gaagatgaac gagatctact tttgaatgag
     1561 ttgaaggtca catatacgat tcttttggat cctcttgctg aaacatatta caaatggggt
     1621 ggactcaatg tcccaaacat gtcgctggtt tccaaagacg gcgtcattgt ttttcagcat
     1681 gacggataca gccccgatac tgcaaaaaac gccgaggagg caataaaaaa agaaatagga
     1741 taggtttaat tttattgttt tggccgaaca aaaacaaaaa cttttcaaac aatgagaaat
     1801 gttacttttt cgtataaaag cattacgcta tatcattact ttctgagttt tacatgtaaa
     1861 aattttttgt tttagttttt gtaaaaagtt acaaaagagc atatacaagc tattgacaga
     1921 aaggtttgtt gatgttatta cacatgtcag caattgggat atcaaacact aaaagataaa
     1981 aagaggtgag ggatgaataa tgtgggtgat atgcggaaaa agttcaaatc gtttattgtt
     2041 atttttgttt attttatgct gttttctaat gtttttgttt ttctgagcac ggccaaagca
     2101 tctcaggctg agcggaacgt gccaccatcg acgaggcctg ttgatggaga aaaagcaaga
     2161 ttcccaaagg aaaaaattga tattacccct gcaggtgctg ggacttataa gttatatcag
     2221 gagaaaaacg gagttttctc aatggtgctc ggtttaatcg gggcacaaga aaaaccactg
     2281 tctggagata aaacaaatat tcaacctaac agaaatcttg tttgcccagc tggaggacct
     2341 ggtcttgttg acaatataac cttggaaaat cgcaatggga ctgtgactac aacagtttat
     2401 ccgacctctt atttcggcgt tcaggatact aatataacaa tacctccacc ccttgtggtt
     2461 gagacaaata catatatcaa atataacata gattattcaa gaaaggtaaa gatcctc
//
LOCUS       DAOSRI010000245         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37685_length_2514_cov_5.689305, whole genome shotgun sequence.
ACCESSION   DAOSRI010000245 DAOSRI010000000
VERSION     DAOSRI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37685_length_2514_cov_5.689305"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>2514
                     /locus_tag="PK717_07035"
     CDS             198..>2514
                     /locus_tag="PK717_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47449.1"
                     /translation="MDTLSIQGVDSWENPYFDSANTAYQDELLNYPVLPSGMFLNGGK
                     IGLPNLGQTNYDYFEYARPILYTQDKTSFKKYALPYRYTIFKDGHLFMSLENDGYSII
                     IDGDSENYSPSYITSAKPIPGTDIVYDFKIGKLSFYDERGLLWEKQPSYGNVSRIWGD
                     LAYCDGEIRNIRTGELYKQQSWGISDGDVCCVDVFERDVKGASQFKHTLVFRCQDGSA
                     IGLIPYHIDKIKSDCLEAYEDDTKTIHKLTSVVRFSKKDCSFIEKLEVSEEQANNGWI
                     LIDSYRNMGLLKSGSNWSIVNLVTGKECLGFKDNQPPESVYFGNDGLMVCVFKFGVQL
                     YDLDAIEPTGKPQIIQTDGKRNNKIYWPESCDFKFSRDNLLYLKTIEDGSQVVSCDPD
                     TLQVKASNGMKLPGVFINTETARSLISRISYDNNKYTKIYEDKGAVSIDLWSVGKSKP
                     LVHLSSDLNGLKHIGPVTWKDGSFFLTFLSEKDKYETFSILQIDSRNGQIIKEYKIND
                     RAFKSYDHDNERLLAVDEKRVVVLNSHNIWCINRQTEEIEKIEIPDLLENFVPILVGN
                     YLLVDDNAMGIVRVVNLAIGEVWKNYYLIGIQNSCAYLLPDKSSIPRKIKIIDSKNNT
                     TTDIVAPQILQNKQYAAVSTCKNAFLMNGAILSNKFDLVQKIPEHSKSESIFKIFGGS
                     AYANKDGSLFKLSPCPSFSVKRTGKGGVRDRGNDKIMGDKDGAGEVSFEFKNTREDGR
                     DLVLKGEVYLVSWGDDGKAPVFAKLNEPRHKL"
BASE COUNT          838 a          465 c          543 g          668 t
ORIGIN      
        1 ggactcctgt tgctcttgac cagcagaagg ccattgccgt gactgtgtgg ggaagacaat
       61 gataataact ttgtatgctg tttttttgaa aacaattctc agtcggcaaa aaaaaggaaa
      121 tgaaaaacat caattacaaa acaaatctat tgttagcaat gcttatcttc gtatcaaatt
      181 tagctggagt ggctcccttg gacacactct ctattcaagg cgtggattca tgggaaaacc
      241 cttattttga ttctgcaaac acagcatatc aggacgaatt gctgaattat cctgtattac
      301 cttctgggat gtttctcaat ggaggaaaaa taggcttgcc aaatcttggg caaacaaatt
      361 acgattattt tgaatacgca cggccaatac tatacacgca ggacaaaacc agcttcaaaa
      421 aatacgcact cccatacagg tatactattt tcaaggacgg tcatttgttc atgtctcttg
      481 aaaatgatgg ttattccata atcatcgatg gagacagcga aaattactcg ccttcttaca
      541 tcacatcagc aaaaccaata ccaggaacag atatcgtata cgatttcaaa ataggcaagc
      601 tctcctttta tgatgagcgt ggccttctct gggaaaaaca accatcctat ggcaatgtat
      661 caagaatctg gggtgatctg gcatattgcg acggagaaat aagaaacata agaacgggcg
      721 aactgtataa acagcaatca tgggggattt ctgatggcga tgtttgttgt gtcgatgtct
      781 ttgagcgtga cgtcaaagga gcaagccaat tcaaacacac gctggtattc agatgccagg
      841 atggctctgc aatcggtttg atcccatatc atattgataa aataaaaagc gactgccttg
      901 aggcgtatga agatgacaca aaaacaattc acaagctaac ttctgttgtg cgtttttcca
      961 aaaaagactg cagttttatc gaaaaactgg aggttagcga ggaacaggcc aacaatggat
     1021 ggatacttat cgattcatat cggaacatgg gactgctgaa atctggatca aattggtcca
     1081 ttgtgaatct ggtcactgga aaagaatgcc tcggctttaa agacaaccag ccacctgagt
     1141 cggtgtattt tggaaatgat ggattgatgg tgtgtgtttt taaatttggc gtgcagcttt
     1201 atgacctaga cgcgatagaa ccaactggca aaccgcaaat catacaaaca gatggcaaac
     1261 gcaataataa aatatattgg ccagaatcgt gcgatttcaa gttctcaagg gacaaccttc
     1321 tctatctaaa aacaatcgag gatggatcac aagttgtttc ttgtgatcct gatactcttc
     1381 aggtaaaggc gtcaaatggg atgaaattgc caggtgtttt tatcaataca gaaacagcca
     1441 ggtcactcat ctcaagaatc agttatgaca acaacaaata caccaaaata tacgaagata
     1501 aaggagcggt gtcgatagat ctgtggtctg ttggaaaatc caaacctctg gtgcatttga
     1561 gctcagatct gaacggatta aaacatattg gccctgtcac ttggaaagat gggagtttct
     1621 ttctaacatt tctctcagaa aaagacaaat atgaaacttt cagtatttta caaatagatt
     1681 ccagaaatgg acaaataata aaagaatata aaataaatga cagagcattc aaatcatacg
     1741 atcacgacaa cgaacgcttg cttgcagtgg acgaaaagcg cgttgtggtt cttaactccc
     1801 acaacatttg gtgcataaac cggcaaaccg aagaaataga aaaaatagaa attccggatc
     1861 tcctggaaaa ttttgtacca atacttgttg gtaattattt gcttgttgac gacaatgcaa
     1921 tggggatcgt tcgggttgtc aatctggcca ttggcgaggt ctggaaaaat tattatctga
     1981 taggcatcca aaacagttgt gcgtatctgt tgccagacaa atcaagcatt ccaagaaaaa
     2041 taaaaataat agattcaaaa aacaacacta caacagacat tgtggcacca caaattcttc
     2101 aaaataaaca atacgcggca gtctcaacct gtaaaaatgc ttttctcatg aatggcgcaa
     2161 ttttgagcaa caaatttgat cttgtccaaa agattcctga gcattcaaaa tctgagtcta
     2221 tattcaaaat ttttggcgga tcagcatatg ctaacaaaga tggttcatta ttcaagctct
     2281 ccccatgccc gtcattctcg gtcaaaagga cagggaaggg tggagtaagg gatagaggta
     2341 atgacaagat catgggtgac aaggatggag caggtgaggt tagctttgag tttaagaaca
     2401 caagggaaga tggaagggac cttgtgttga aaggggaggt gtatctggtt tcttgggggg
     2461 atgatgggaa ggcacctgtg tttgctaaat tgaatgaacc tagacataag ttag
//
LOCUS       DAOSRI010000246         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37790_length_2510_cov_3.727088, whole genome shotgun sequence.
ACCESSION   DAOSRI010000246 DAOSRI010000000
VERSION     DAOSRI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37790_length_2510_cov_3.727088"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK717_07040"
     CDS             complement(<1..365)
                     /locus_tag="PK717_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47450.1"
                     /translation="MMKRTSTFLAILAIILSLFVNIPSPSYARTEKISNFNIWYFKPS
                     QVSTSATYKVLIDINETVGIHGWLKVTFPPEWTMPDVPPKGGYSEYWDHELERILTSI
                     YIGTSPCTKCQGLPEIKTN"
     gene            606..1787
                     /locus_tag="PK717_07045"
     CDS             606..1787
                     /locus_tag="PK717_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR47451.1"
                     /translation="MATFFAGLGAGINEVNFNYYLLSLGYNEIFIATLTSLGTLTAAI
                     ASIPMSFLVNKVGRRNAIVINQLISWPLFLLQVLLPISPIILGASVVSSLVSTMQGLS
                     ERPLLIESTEEGKRVNVFSLVTMTSLVAVISGNFFGGKLTYLATQMFSLPAESPWAYR
                     AALFCTSLLSGIAMNCYLRLKHVPISADHLDTRTNSHFFKRLFGNLESFGFIWKLLLG
                     QMLIGFGAGFTVPLFSLFFKKVYHATADQWGTISSIAKLPMFFGVMISPKIARKFGSL
                     KTVVFCQYLSIPFLLLTLYSPNIAVAGFGFAIRHALMNMTEPIWASFYMGMLKPKELS
                     TVSSFITVGWNTLNATGTRIGGSFLESGKHQWSFYITGLAYIIASSFYSIVWRKHMRK
                     T"
     gene            complement(1839..>2510)
                     /locus_tag="PK717_07050"
     CDS             complement(1839..>2510)
                     /locus_tag="PK717_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47452.1"
                     /translation="SSDQKSLDFGMIKQGENPTKQITIKNSGNGVLDGTIELQGNWVE
                     VSRNKLTGNETIFTVKAFSEKMEYWGSTAKITIKSNGGNIEIECKGIKKGKLIILQVN
                     SPTATVDDKQVSMANPPSIIGGSTFVPLRFIGEALGAKVDWDANEKKVTYVLADRTVV
                     LWIGRKTALVNNKEVEFTAAPVIVKGSTMVPLRAVGEALGATVEWIAQSKTINIYYPP
                     NPKIG"
BASE COUNT          672 a          552 c          497 g          789 t
ORIGIN      
        1 ttgttggtct ttatttccgg aagaccctgg cattttgtgc atggagaggt tccaatataa
       61 atggaagtca atattctctc aagctcatgg tcccaatatt cggaatagcc accttttggc
      121 ggaacatccg gcatagtcca ctctggtggg aaagtcacct taagccagcc atgtattcca
      181 accgtttcat taatatcaat aagaacctta tatgttgccg atgttgaaac ctgtgatggc
      241 ttgaagtacc agatgttgaa attcgatatt ttttcagtcc ttgcataaga gggtgacggg
      301 atattgacaa acaacgagag aattattgcc aggattgcca ggaacgtaga agttctcttc
      361 atcataaatg cctccttaaa aagctttatt acagactatt ttacctttcc acaagtacaa
      421 ccttaagcct gttaaggttt tcaaaacata agctcattct tgactttgtt tctttacttt
      481 gttaacattt gcgaagccac aaaatgcaga taaatccaac gcaatttaag ttcaatggac
      541 aatatcgcca aaaccaaatt tttacttttt ttaagacata tccaataaac acaaggctta
      601 ttttaatggc tactttcttt gcgggtcttg gtgccggaat aaatgaagtc aacttcaact
      661 attacctatt gtctctcgga tacaatgaaa tttttattgc tacgctcacc tctctcggaa
      721 cattaacggc agcaattgct tccataccaa tgagctttct cgtcaataaa gtaggcagaa
      781 gaaacgcaat tgtaattaac cagctgattt catggccatt gtttctattg caggtcctat
      841 tgccaatttc tccaataatc ctgggcgctt cggtagttag ctcgctggtc tcaacaatgc
      901 aggggctttc agaaagaccg cttctcattg aatcgactga agaaggcaaa agggtaaatg
      961 ttttttctct tgtgaccatg acatccctgg ttgccgtaat ttccgggaat ttctttggtg
     1021 gaaaattaac ttatcttgca acgcaaatgt tctccctgcc cgccgaatcg ccatgggctt
     1081 acagggcagc gcttttttgc acatcccttc tctctgggat agccatgaac tgttacttga
     1141 gactaaagca tgtcccgata agtgcggacc acctagacac aagaacaaac agccattttt
     1201 tcaaaaggct cttcggcaac ctggagagtt ttggtttcat ctggaagctg cttctgggac
     1261 aaatgctcat tggatttgga gcaggattca ctgttccact cttctccctt ttcttcaaaa
     1321 aggtgtatca tgccaccgcg gaccagtggg gaacaataag ttccatagcc aaattgccaa
     1381 tgttttttgg cgtcatgata tccccaaaaa tagcgaggaa gtttggcagc ttgaaaactg
     1441 ttgtgttctg ccagtacttg tccataccat ttctcttgct cacgctttat tctccaaaca
     1501 tagctgtggc aggttttggg tttgcaataa gacatgctct tatgaacatg acagaaccaa
     1561 tctgggcaag tttctacatg ggtatgttga aaccaaagga gcttagcact gtttcaagct
     1621 ttataactgt tggatggaac acattgaatg ccacaggaac aagaattggt ggaagcttcc
     1681 tagaaagcgg caaacaccag tggagtttct atataactgg cctcgcctat atcattgcat
     1741 caagtttcta ttcgattgtc tggcgaaaac acatgcgcaa aacataaaac aaaaaagggc
     1801 cgcacaaagc ggcccttttt tgtttaagct aatttagatc atccaatctt cgggtttggc
     1861 gggtagtata tattgatcgt ttttgactgg gctatccatt caacagttgc gccaagcgct
     1921 tcaccaactg ccctcagtgg gaccattgtg cttcctttga caattactgg tgctgctgtg
     1981 aactcaactt ccttattatt gacaagtgcc gttttacgcc cgatccagag aacaactgtt
     2041 ctgtctgcaa gaacatacgt cacttttttt tcattggcgt cccagtcaac ctttgcgcca
     2101 agtgcctctc cgatgaaacg aagtgggaca aatgttgaac caccaataat tgatggtggg
     2161 ttggccattg aaacctgttt gtcatcaaca gtggctgttg gtgaatttac ttgtaaaatt
     2221 atgagttttc cctttttaat gcctttgcac tcgatttcga tattgccacc attgcttttt
     2281 atggtgattt tggcggttga gccccaatat tccatcttct ctgaaaacgc ctttaccgtg
     2341 aatatagttt cattgccggt caatttattt ctggagactt ccacccagtt accctgaagt
     2401 tcaattgttc catcaagaac accgtttcca gaattcttta tggttatctg ttttgttggg
     2461 ttctctcctt gtttgatcat tccaaaatca agagattttt gatctgagga
//
LOCUS       DAOSRI010000247         2508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_37821_length_2508_cov_4.238891, whole genome shotgun sequence.
ACCESSION   DAOSRI010000247 DAOSRI010000000
VERSION     DAOSRI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37821_length_2508_cov_4.238891"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2203)
                     /locus_tag="PK717_07055"
     CDS             complement(<1..2203)
                     /locus_tag="PK717_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47453.1"
                     /translation="MGQYRTRKRKLGLVCQCIALSLILVFGQGVSAITGNQDSCEGIT
                     YNLSTNSQDQGVAFPLLPYSGGNQIPYNPEYLISDQYYIKYTGFGCSCIDKHTGLELP
                     ASLFPTKIIDTDRESLKIIQNCGILVEFSRIIQFDEKRFCATKQENFKEYLLMFDQDG
                     IKWKTHFDDASSYPLFAPLSPNRILYGPNIVDLETGKVLLELDRKYKPLEQRDVIKKG
                     GIAVINKSIVIDVEKPAVLWTLPEDPKYHFNVMGFYEDKLIYFNTSNNLVSYYDPSSG
                     ELLKTFEFKPRLEPGERIGRIDGLAGKSIYSEVGYVIDIESGILLTQKNHDLLKDKWG
                     HFAGIDEKWLFWEDIAGIECLDPKTGKTLWRREISPYVFYQELEGKIYKNTFHLADKD
                     NNIKSSFIESLSILEDGSWRELAKAPLRSYLRPFISKNGLSLIPTKYGILWLPQIQHD
                     SAGPSIIAPGTSQIQRTYLIPDWDRHSWQNTPGYALTGDTLIVNLDGYGIHLVDLASG
                     KTEQIKIPDASNSDKHGFYKSVPIFADKKYAITYDEHMRIVCFDITKKVFLRRMESTD
                     KKIHLSLDYGYNHSIVGEWFSIGNYITNLETGEIIDLGSNVICATNNGFFIIKDDLSL
                     VVPEDEPRKIIDKSELFYKLFGGMGIFWKTTVNYAICNTGVIDFATNNIVQKFFKNTM
                     ENRVPFIFQDELLLTASSNTVKLSPCPSFSVKRNGKTTSGDNEVSFELRNTR"
BASE COUNT          705 a          530 c          450 g          823 t
ORIGIN      
        1 cccttgtgtt ccttaactca aagctaactt cattatcacc gcttgtggtt ttaccattcc
       61 ttttgaccga gaatgatggg caaggggaga gcttgacggt attacttgag gcagttaata
      121 acagctcatc ctgaaaaata aaaggtactc tgttttccat cgtgttttta aagaattttt
      181 ggacaatatt atttgtagca aagtcaatta caccagtatt gcaaatggcg taattgactg
      241 tagttttcca aaatatgccc atacccccaa ataatttata aaataattct gatttatcta
      301 ttatctttct tggctcatcc tctggcacaa ctagtgataa atcgtctttt ataatgaaaa
      361 aaccattatt agttgcacag attacattgc taccaagatc gataatttcg ccagtttcta
      421 aatttgttat gtaattgcca attgagaacc attctccaac aattgagtga ttgtacccat
      481 aatccagaga taagtgaatc tttttgtctg tcgattccat ccttcttagg aaaacctttt
      541 tggtgatatc aaagcagaca atcctcatat gctcatcata tgttatggcg tattttttat
      601 ctgcaaatat tgggactgat ttataaaaac catgtttgtc actgttactt gcatctggaa
      661 tttttatttg ctccgttttg cctgatgcaa gatctacgag atgtatgcca taaccatcga
      721 gatttactat aagtgtgtca ccggttaggg catatccagg agtattttgc caagagtgcc
      781 tatcccaatc aggtatcaag taggttcttt ggatctgtga tgttcctgga gctattattg
      841 acggtcctgc ggagtcgtgc tggatctgtg gtagccacaa tattccatat tttgtcggta
      901 ttaacgaaag accatttttt gaaatgaatg gtcgtaaata actccgtaat ggcgcttttg
      961 ctaattctct ccatgaacca tcctcaagga ttgaaagtga ttcaatgaag ctgctcttta
     1021 tgttgttatc tttatctgcc aaatggaatg tgtttttgta aatttttcct tcaagttcct
     1081 ggtaaaaaac ataagggctt atttctcttc tccagagggt ttttcctgtt ttagggtcca
     1141 ggcattcaat tcctgcaata tcctcccaga agagccattt ttcatcaata cccgcgaagt
     1201 gcccccactt atcttttaag agatcatggt ttttttgagt caaaagtatt cctgactcaa
     1261 tgtctataac atagccgacc tcagaatata ttgacttgcc agcaagacca tcaatccgtc
     1321 cgatgcgttc ccctggttcc aggcgaggtt tgaactcaaa tgttttcaag agctccccag
     1381 atgaagggtc atagtaagaa acaagattgt tgcttgtatt aaaataaatc aatttatctt
     1441 catagaaccc cattacattg aaatgatatt tggggtcttc cgggagggtc cacaaaacag
     1501 ctggtttctc tacatcgatg acaatcgatt tatttataac agctatcccc ccttttttta
     1561 taacatctct ttgttctaat ggtttatatt tcctgtccag ctccagcaac acttttcctg
     1621 tctccaaatc aacgatgttt ggaccataga gtatcctgtt gggactcaat ggagcaaaaa
     1681 gtggataaga tgaggcatca tcaaaatggg ttttccattt tatgccgtct tggtcaaaca
     1741 ttagaaggta ttctttgaaa ttttcctgtt ttgtggcgca aaaccttttt tcatcaaatt
     1801 gaataattct tgaaaattca accaatatac cacaattttg gatgatcttt aaactttccc
     1861 ggtcagtgtc gattattttt gttggaaaca aagaagctgg caattcgagg ccagtgtgtt
     1921 tatctatgca agaacaacca aaccctgtgt attttatata atattggtcg gaaataagat
     1981 attctgggtt atatggaatt tggtttcctc cagaatacgg caaaagcggg aaagcaaccc
     2041 cctggtcctg cgagtttgtg gagagattgt atgtgattcc ttcgcagctg tcctggtttc
     2101 cagttattgc gctgacacct tgtccaaaaa caagaatcaa tgacaaagca atgcattggc
     2161 aaaccaaccc caattttctt tttcttgtcc tgtattgtcc catatcaaaa acctccttta
     2221 ttatccatgg atgcaaccca tatcagtaca cccagagtca tctacaacca gactcctgca
     2281 actgccgtct gtctcagttg ttgcgcatgg aatagtagat gtcccaatat ccacacaata
     2341 ctcattgctg catccatcat ctgagcaagg caacctacct tccccacaca gtcacggcaa
     2401 tggccttctg ctggtcaaga gcaacaggag tcccatcaaa aagaggcctg ggatctttat
     2461 catactcaga aagaacagac ttctctctgt ccattaatcc attagact
//
LOCUS       DAOSRI010000248         2467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_38731_length_2467_cov_3.112355, whole genome shotgun sequence.
ACCESSION   DAOSRI010000248 DAOSRI010000000
VERSION     DAOSRI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38731_length_2467_cov_3.112355"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1002
                     /gene="dusB"
                     /locus_tag="PK717_07060"
     CDS             13..1002
                     /gene="dusB"
                     /locus_tag="PK717_07060"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOR47454.1"
                     /translation="MTKTWSIGNKVIQGPLVLAPLAGIGDIPFRMLCKRYGVDLVYTE
                     FVSAQGLLHKNKGSFKMLKVEDLEHPVTIQLFGSRPEHLAEAVKYVEQAGADFVDLNC
                     GCPEPKVVRTGSGSALMSDPGLIARIVEAMVKSTSLPVTVKTRIGTRRGFPIAAEIAR
                     LCEMAGASAFALNCCYVSQGFVGPFDWEYIRTIKQTVKIPVIANGGIKNGKDVLEVLD
                     KGADAAMVGRVTLGAPWAFEWIRDEMDGLGKRTIPLKERFSVMMEHLGLEVEMRGEKI
                     GVSEMRKHWAWYVKGLPGASKFREEAVAGKTLQQMNDTLSRFREYLERQGEER"
     gene            999..1469
                     /locus_tag="PK717_07065"
     CDS             999..1469
                     /locus_tag="PK717_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47455.1"
                     /translation="MKKGLVLLVCAIFVSGYGSAISYDSRTANGLLKKELMKVPSIYP
                     VYSHGMITTDTQQPFATSNEKFEPRIQGTLVFSEIGEDPVAWYNKKLIEEGWNVSEVV
                     FGSSENMAGKNTFPNLATGEPARLVASQGKKQLQLVFRLLDTGFSVTHFGVEAP"
     gene            complement(1473..2213)
                     /locus_tag="PK717_07070"
     CDS             complement(1473..2213)
                     /locus_tag="PK717_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47456.1"
                     /translation="MGKKLLWGFAITLGLIALIITAFVLFNPGGKPNNPYSQLYLTVV
                     PVNSEKPDWETLKKAIEARVSYLGELRFFENKESYARVVVTGSEDPNEMGSMLLVQGK
                     TLLITDKGQKIATDDEFQSAMPVSFKEKTGTYGIAIQLNDTGKQRILAALGAKDKKPS
                     KIQLILDEIPVGIMDIKDIGQDGTLSFHLKDGMRKSFAELLSRVFITRLPFKIAGIVN
                     SSYFIAPQSDVESPLNFVDFLYEARIDK"
     gene            complement(2213..>2467)
                     /locus_tag="PK717_07075"
     CDS             complement(2213..>2467)
                     /locus_tag="PK717_07075"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR47457.1"
                     /translation="DGLIVELLEPTSDDSPIKGFLDKRGPGIHHIALEVDDVDHKIEE
                     FLNAGVKMIDKKSRIGAAGKPIAFIHPSSVGGVLVELEQD"
BASE COUNT          658 a          494 c          611 g          704 t
ORIGIN      
        1 taaaggccct ttatgacaaa aacgtggagc atagggaata aggtaataca gggcccgctt
       61 gtattggccc cactggctgg cattggtgac ataccatttc gaatgttgtg caaacgatat
      121 ggtgtagatc ttgtctatac cgagtttgtg tcggcacagg gtcttttaca caagaacaag
      181 ggttctttta aaatgctcaa ggttgaagac ttggagcacc ctgtcacaat ccagcttttt
      241 ggttcaaggc cggaacacct tgccgaagca gttaaatatg tagagcaggc cggggcggat
      301 tttgttgatc ttaactgtgg ctgtccagaa ccaaaggtgg tcaggactgg ctccggatcg
      361 gccttgatga gtgaccccgg tttgattgct cgcattgttg aggcaatggt caaatcaacg
      421 tcacttcctg taactgtcaa gacaaggatt ggcactcgca gaggatttcc gattgctgct
      481 gaaatagcca gattgtgtga gatggccgga gcatcggcat ttgcgctcaa ttgctgctat
      541 gttagccagg gatttgtggg cccattcgat tgggaatata tcaggacaat aaaacagact
      601 gtgaagatac ccgtaatagc caatggcggc ataaaaaatg gcaaggatgt gctggaagtt
      661 cttgataaag gtgcagatgc ggcaatggtg ggaagggtga cacttggagc gccatgggct
      721 tttgagtgga ttagagacga aatggatgga cttggcaaaa gaactattcc tctcaaagag
      781 aggttctcag tgatgatgga gcaccttggt cttgaggtgg agatgagggg tgaaaaaatc
      841 ggggtcagtg agatgagaaa acactgggcg tggtatgtga aaggtcttcc tggcgcatca
      901 aaattcaggg aagaggcagt agcagggaaa actttgcagc agatgaatga caccctttca
      961 aggttcaggg aatatttaga gaggcaaggg gaggaaagat gaaaaagggt ttggtgcttc
     1021 ttgtttgtgc aatttttgtt tctggttatg gatctgcaat atcttatgat tcaaggactg
     1081 caaatggatt attaaagaaa gagcttatga aagtgccctc aatttatccc gtctatagcc
     1141 acgggatgat taccactgat acccaacagc cttttgccac cagcaatgaa aaatttgagc
     1201 ccaggataca gggaacgctg gttttttctg agattggcga ggaccctgtt gcatggtaca
     1261 acaaaaaact tatagaggaa ggttggaatg tttccgaggt tgttttcggt tcatctgaga
     1321 acatggctgg gaaaaatact tttccaaacc tggctactgg ggagccggca agattggtgg
     1381 catcacaagg gaaaaagcag cttcagctcg ttttcaggct cctggataca ggattttctg
     1441 taacccattt tggagttgaa gcaccctaga ttctatttgt ctattctggc ctcataaagg
     1501 aaatcgacaa agtttaatgg tgattccaca tctgattgcg gggcgatgaa gtaggaagag
     1561 tttacgatgc ctgctatttt gaatggcagc cttgtgataa atacccttga gagcagctct
     1621 gcaaaagatt ttctcatacc atctttaaga tgaaagctca atgtgccatc ttgaccaatg
     1681 tctttaatgt ccattattcc aactggtatt tcatccaaaa taagctggat ttttgatggt
     1741 tttttgtctt tggcgcctag cgctgccagg atcctttgtt ttccagtatc atttagctgg
     1801 attgcaatcc cgtaagtgcc agttttttct ttgaaagaaa ctggcatggc cgattgaaat
     1861 tcatcatcag ttgcaatttt ttggcccttg tcggtgataa gtaacgtttt cccctgaaca
     1921 agcagcattg atcccatttc attcgggtct tctgaaccag taaccactac cctcgcgtag
     1981 ctttccttat tttcaaaaaa ccttaattca ccaaggtaac tcactctggc ttcaatggct
     2041 tttttaaggg tttcccagtc tggtttctcg ctgttcacgg gaacaactgt gagataaagt
     2101 tgggaatagg gattgtttgg tttcccccct ggattgaaaa gtacaaaagc agttattatg
     2161 agggctatca accccagcgt gatggcaaat ccccagagga gctttttacc cattagtctt
     2221 gctccagttc cacaagcact cctccaactg aagacggatg tatgaatgca attggttttc
     2281 ctgccgctcc aatgcgggat tttttatcaa tcattttcac tccggcattc aggaattctt
     2341 ctattttatg gtcgacatcg tcaacttcta atgcaatatg gtgtattcct gggcccctct
     2401 tgtccagaaa cccctttata ggtgaatcat cagaagtggg ctcaagaagc tcaactatca
     2461 aaccgtc
//
LOCUS       DAOSRI010000249         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_38791_length_2463_cov_4.813538, whole genome shotgun sequence.
ACCESSION   DAOSRI010000249 DAOSRI010000000
VERSION     DAOSRI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38791_length_2463_cov_4.813538"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PK717_07080"
     CDS             <1..839
                     /locus_tag="PK717_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR47458.1"
                     /translation="SNWDYIIRPQRQWFDLRLGELWRARNLIMLFVWRDFVTYYKQTI
                     LGPLWYLIQPLLTSLTFTVVFGNFAKLSTDGLPQFLFYLAGTVVWGYFSACLTKTSNV
                     FTSHANIFGKVYFPRLTVPISILLSNLITLLIQFLLFLAFMIYFSSSGADVHPNATIL
                     LLPILLILMAGLGLGFGIIISALTTRYRDLQFLVTFGAQLWMYITPVVYPLSSVPETY
                     RWLVLANPMTSIVETFRFAFLGAGIVETTHLLYSLVCMIVVMLIGILIFNRVERTFMD
                     TV"
     gene            841..2133
                     /locus_tag="PK717_07085"
     CDS             841..2133
                     /locus_tag="PK717_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47459.1"
                     /translation="MSSVIVFDGVSKCYRLGSIGNQTLYQDLNRWWAKVRRKPDPLLK
                     IMDARDGKDGDQFIWALRNINLEIQHGEVVGVIGRNGAGKSTLLKILSQITAPTTGEI
                     KIKGRIASLLEVGTGFHPELTGRENIYLNGALLGMSKEEIQRKFDEIVDFSDVEQFID
                     TPVKRYSSGMYVRLAFAVAAHIEPDILLVDEVLAVGDAQFQKKCLGKISDVSQKEGRT
                     VLFVSHNMGMITSLCKKAVLLDNGCILVEGNVTDVVTRYYNAGISSPYAVDFRKQGKK
                     VGDQYATLLEVHIEDMEGNLIGEVDIRAPFKLKMTYDLHGAPVAPRPNFYVISPQGQY
                     VFVTGGGEKGKAIDGTYEATCIVPGNLLNNETYTIDAALLYTDSIFHYSFHERGAVMI
                     TVIDPIDETIEETTRYGMSGPLPGAVRPKLPWEVRKIA"
     gene            2178..>2463
                     /locus_tag="PK717_07090"
     CDS             2178..>2463
                     /locus_tag="PK717_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47460.1"
                     /translation="MIETIRNDGAIVAIVIYSDYSVEGIKFISPNDFSLQLGTMSRPA
                     GYKVLPHIHNPVERHCVGTQEVLFIKSGKIRIDFYSFDKVYLESRELAAGD"
BASE COUNT          649 a          518 c          600 g          696 t
ORIGIN      
        1 acagtaattg ggattacatc attcgtcctc aacgacaatg gtttgacctg agactgggtg
       61 aactgtggcg ggcgcgtaac ctgatcatgc tgtttgtctg gcgcgatttc gttacctatt
      121 ataaacaaac cattttggga cccctttggt atctgattca gccactactc acatctttga
      181 ccttcacagt tgtatttgga aatttcgcga aactgtcaac ggatggactt cctcaatttt
      241 tattctattt ggccggcact gtcgtttggg gttatttctc tgcgtgtctg accaaaacgt
      301 ccaatgtctt tacctcgcat gcaaacatat ttgggaaggt atatttcccc cgactcacag
      361 tgcctatctc tatcttgttg tcgaatctga ttacgttgct tatacagttt cttctcttcc
      421 ttgctttcat gatctatttc agttcttccg gcgccgatgt ccatcccaat gcaaccattc
      481 ttctattgcc aatacttctc atcctcatgg cagggctcgg tctcggcttt ggcattataa
      541 tatcggcact taccactcgt tacagggacc tgcagttcct tgtgacattc ggtgcccaat
      601 tgtggatgta cattaccccg gttgtctatc ctctatcctc ggttccggaa acgtatagat
      661 ggcttgtgct ggccaacccg atgacttcca ttgtggaaac gtttcggttt gcttttctcg
      721 gtgccgggat cgtggagacc acccacctgc tgtacagctt ggtatgtatg attgtggtta
      781 tgctcatcgg aattctgatc ttcaaccggg tcgagagaac cttcatggat acggtataat
      841 atgtcttctg ttatcgtatt tgatggggtt agcaagtgtt acagattggg aagcatcggc
      901 aatcaaacac tgtatcaaga tctgaatcgc tggtgggcta aggttcggcg caaacccgat
      961 cccctactaa agatcatgga tgcaagggac ggcaaggatg gagatcaatt tatatgggca
     1021 ttgcgtaata tcaaccttga aatacagcat ggtgaggttg ttggagtcat cggtagaaat
     1081 ggcgccggga aaagcaccct gctcaagatc ctttctcaaa taaccgctcc tacaaccgga
     1141 gaaataaaga tcaaagggcg aatcgcgagt ttattggaag ttggcaccgg gtttcatccg
     1201 gaattgacgg gaagagagaa tatctatctc aacggtgcct tacttggcat gtcaaaggag
     1261 gagattcaac gcaagtttga tgaaatagtc gatttctctg atgtggagca atttatcgat
     1321 acgccggtta aacgctattc aagcggcatg tatgtgcggc tggcttttgc agttgccgcc
     1381 cacattgaac cagatatcct attggtcgat gaggttctgg ctgttggtga tgctcaattt
     1441 caaaaaaaat gtttgggaaa aatcagcgat gtatcgcaga aggaaggacg tacagtattg
     1501 tttgtcagtc ataatatggg gatgattacc tccttgtgta aaaaggcagt actcctcgat
     1561 aatggctgta ttttggtgga aggtaatgtc acggatgtcg tgacaagata ttataacgca
     1621 ggcataagtt ctccatatgc ggttgatttt agaaagcagg ggaaaaaagt gggggatcaa
     1681 tacgcaaccc ttcttgaagt gcatatagag gacatggagg gaaatctcat tggggaagtg
     1741 gacattcggg cgcccttcaa actcaaaatg acctatgacc ttcatggcgc acccgttgcc
     1801 ccgcggccca atttttatgt catcagcccc cagggccaat atgtctttgt gaccggggga
     1861 ggggagaaag gcaaagcaat agacggaact tatgaggcaa cctgcattgt cccaggtaat
     1921 ttgctcaata atgaaacata tacaatcgat gccgcccttc tctatacgga cagcattttt
     1981 cattacagtt tccatgaaag aggggcagtc atgatcacgg taatcgatcc catagatgaa
     2041 acaattgaag agactaccag gtacgggatg agtggaccgt tgccgggggc cgttcgccca
     2101 aagctcccgt gggaagtgag aaaaatagca tgaatataag tattaaggca caaaaataaa
     2161 ttgcaaggtg aatgttgatg atcgaaacaa ttaggaatga tggagcgata gttgcgatag
     2221 ttatctatag tgactattcg gtggagggaa taaaatttat atctcccaac gacttttcat
     2281 tacaacttgg caccatgagc cgcccagccg gttacaaggt attacctcat attcataacc
     2341 ctgtcgagcg gcattgtgtg ggaactcaag aagtgctttt tataaagagt ggaaaaataa
     2401 ggattgattt ctattccttt gacaaagttt accttgagag ccgtgagttg gcagcggggg
     2461 ata
//
LOCUS       DAOSRI010000250         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_38874_length_2459_cov_3.749584, whole genome shotgun sequence.
ACCESSION   DAOSRI010000250 DAOSRI010000000
VERSION     DAOSRI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38874_length_2459_cov_3.749584"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK717_07095"
     CDS             complement(<1..438)
                     /locus_tag="PK717_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47461.1"
                     /translation="MKKILSVIFCFLISLSCLSFAGCGSSNDAKMAIDTLKAIGEGKW
                     DAVFNNLHTACLYTQNKNEFVLQMPYHAKALSEFFKGATFEVVDEKGQDSWTYRGQML
                     EGANKVTLRVTFASKADPPSAFPFAMEFSQTNTVDFVVAKADGG"
     gene            complement(446..2173)
                     /locus_tag="PK717_07100"
     CDS             complement(446..2173)
                     /locus_tag="PK717_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47462.1"
                     /translation="MKKHLAMVLILLALTGLLLFLQLRSPLALNMDGGYNAANVKALM
                     SFKPMPYDGSPPIPFVLSAIFGFIFSSASIGIKIVVTMSLVAVGLLIYLFVRKITGDD
                     IAAIAALVGWCVSTAIITYPMGYLKQTVALPFLVGGLMSLYLLLTEKKKLLWGLLFAL
                     SFPLVYLSHSPSFLALLASSYVVAAVMLAFGEAKTQKIFGFSMLTLLALGLASTPWTL
                     PILAKKMIQIDPKGYSTIAIYLKGLFAFRFEQTVDFKGFDWLLAAVPVFGLAFCARAG
                     WKRLTLWSLSFLAGIFVLSCFLAKYEWQGRNVMTMLVPLSMMMGLGFYEASGLLFKEK
                     LQKTTAAVLIALCVFLMAGYQNTAIKYAKTGKPIITEEQLSDLSAFISPLDQSIKENL
                     YARHGLRFWATYATGDPVGVFFYDWDRKTFAIRNSRGEEDVGESRKPERKDYLLVSKR
                     PLFGCRIEKLSAPNFKAYVPEQAQEYIKVTVRTTNPIRSLRVTFKDSMWRLVYQQDMK
                     NVLPGEYLDFGKLLNGIKRGTYYLQVVGDNHSTVPFVVNVTENLLSYSEKPFGAVNES
                     KDFVLVRIK"
     gene            complement(2170..>2459)
                     /locus_tag="PK717_07105"
     CDS             complement(2170..>2459)
                     /locus_tag="PK717_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOR47463.1"
                     /translation="PQENRVYAYLSGQTIDVVTTMNARELHHFFSLRCCNRAQWEIRD
                     YAIKMLGAIKKVAPIIFEKAGPSCYRWGECREGRQSCGKAKEVVQFFKGAI"
BASE COUNT          701 a          594 c          541 g          623 t
ORIGIN      
        1 tcccccatct gcttttgcaa caacaaaatc aacagtattt gtttgggaaa actccattgc
       61 aaaaggaaat gcagatggcg ggtcagcctt ggacgcgaaa gtgaccctca gagttacctt
      121 gtttgctccc tcaagcatct ggcccctgta tgtccaggaa tcctgaccct tctcgtcaac
      181 cacttcaaag gtcgcacctt taaaaaactc cgagagggcc ttcgcatgat atggcatctg
      241 caggacaaat tcattcttgt tttgggtata caggcaagct gtatgaagat tgttgaacac
      301 agcatcccat tttccctctc caatagcttt tagtgtatca atggccattt tagcatcatt
      361 ggagcttcca cagcctgcaa aagaaaggca ggagagggag attaaaaaac aaaatataac
      421 tgacaatatt ttcttcattt tgacctcatt ttattcgcac aagcacaaaa tctttagatt
      481 cgttcacggc accaaaaggc ttctccgagt atgagaggag attttctgtg acattgacca
      541 caaaaggaac tgtggaatgg ttgtcgccta ccacctggag atagtatgtt ccacgcttga
      601 tgccattgag caattttcca aaatccagat actccccagg caaaacattt ttcatgtcct
      661 gctggtagac aagcctccac atggagtcct tgaaagtcac cctgagtgat ctgatggggt
      721 ttgttgttct gacagtcacc ttaatgtatt cctgggcctg ttctggaaca taggctttga
      781 aatttggggc agacagtttt tcaatgcggc aaccaaaaag gggtctcttg gagacaagaa
      841 gatagtcttt cctttcaggt tttctgctct cgcccacatc ctcttcaccc ctgctgtttc
      901 ttattgcaaa agtttttctg tcccagtcat agaaaaacac accaacaggg tcgcctgtcg
      961 catacgtcgc ccaaaacctc aatccatgcc ttgcgtaaag gttttccttt atggactggt
     1021 caagtggcga gataaaagct gaaaggtcag agagttgctc ttcagttatg atcggcttgc
     1081 cagtttttgc atattttatt gctgtgtttt ggtaacctgc catcaggaag acgcaaagcg
     1141 cgataagaac agccgccgtt gtcttttgga gcttttcctt gaaaagtaat ccagatgcct
     1201 cgtaaaaacc aaggcccatc atcattgaca ggggaacaag catagtcatc acatttctgc
     1261 cctgccattc gtattttgca aggaagcacg acagaacaaa tatccctgcc agaaaactta
     1321 gtgaccagag ggtaagcctc ttccatcctg ctctggcgca aaaggcaagc ccaaaaacag
     1381 gcactgctgc caggagccag tcaaacccct tgaaatcaac agtctgctca aacctgaagg
     1441 caaaaaggcc tttcaaatag atcgcaatcg tactgtaccc ttttgggtcg atctggatca
     1501 tctttttcgc aaggatgggc agtgtccatg gcgtggaggc cagacctagg gcaaggaggg
     1561 tcagcattga aaaaccaaag attttttggg tctttgcttc accaaaagcc agcatgaccg
     1621 ctgcaacgac atacgatgag gcaagcaggg ccagaaaaga cggagaatgc gagagataga
     1681 caagcgggaa ggagagcgca aaaagcaggc cccataaaag ttttttcttc tcggtaagca
     1741 aaagatacag tgacatcaga ccaccaacaa ggaacggcag ggcaactgtt tgtttgagat
     1801 aacccatcgg atacgtgatg atggccgtgc tgacacacca gccaacaagg gcggctattg
     1861 ctgcaatatc atcaccagtg atttttctga caaacaggta tatcagaaga ccaactgcaa
     1921 caagcgacat ggttacaaca atctttatgc caatactggc cgatgagaag atgaaaccaa
     1981 aaattgccga gagaacaaaa ggaatgggcg gagagccatc atagggcatg ggcttgaaag
     2041 acatcaatgc ctttacattt gctgcattgt atccaccatc catgtttaat gccaatgggg
     2101 accttaattg gaggaacagc aataaacctg tcagggccaa caaaatcaaa accattgcaa
     2161 gatgtttttt caaattgccc ccttgaaaaa ctggacaact tccttggctt tgccgcagga
     2221 ctgtcttcct tccctgcact ctccccaacg atagcacgat ggaccagcct tttcaaaaat
     2281 tattggtgcg acctttttga ttgccccaag catctttatt gcgtagtccc ttatctccca
     2341 ctgcgccctg ttgcagcacc tgaggctgaa gaagtggtgc agttccctag cattcattgt
     2401 cgtgacaaca tcaatggtct gcccggaaag ataggcatag accctatttt cctgtggga
//
LOCUS       DAOSRI010000251         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_39300_length_2439_cov_3.883389, whole genome shotgun sequence.
ACCESSION   DAOSRI010000251 DAOSRI010000000
VERSION     DAOSRI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39300_length_2439_cov_3.883389"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK717_07110"
     CDS             <1..711
                     /locus_tag="PK717_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR47464.1"
                     /translation="INIIETLKNEGTEDSQARAENVEEFLNLSYEFGKTSEDSSLEAF
                     LGTVTLMSDMDNVEEGEQRVIMTTIHQAKGLEFPVVFLCGLEEGTFPHLRSLDDQESV
                     EEERRLAYVGMTRAKEVLFLTRVINRQLWGGTRLCEESRFLKEIACPEIVYEGIKKQT
                     QTAREQQRSFVKKSAQVVITPKKEPIKVSTGEVVVHQAWGDGTVLNVSGDNAEVDFLS
                     VGRKLLNLRYAPIKSKGA"
     gene            807..2117
                     /locus_tag="PK717_07115"
     CDS             807..2117
                     /locus_tag="PK717_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47465.1"
                     /translation="MKKALLLLLCVVTLFSGVVFADGEWKPYLDILVWSNPPDLGIRL
                     QTEIERHFPNIASYLSVVTPASLREPLQYNGYNVICLLGCAKAGQVLDVPEDVETLIE
                     DRCKFGISGLIISGDVIHPDSNNPILQKLAGAQLMDVEKAGRTTVRVIDITDPIVSDV
                     AQSFDVTLGDIRKSALYPGTKSIAVTNSGVPVVFKRNITQSKACYIALGQNGQAVENI
                     EVSRLFANALYDCMGTGWLQPYEAPTNVKAIIGDEKVKITWDKPKKMEGIAGYRVFRE
                     SENEKSKTIHDFALDTETLFFEDSSVTNGKLYTYKVCSIGNADKIVACSHQVQARPGK
                     IQVTLPGWPAQGQPVQVIGSKHIVRGKTRPGSKVIVKWKLIPSDLRVKVRVKQTKTEI
                     LRYRLILPQARKLNITLLLKTSLASRKNSAHILWIARQTRSSFQ"
     gene            2114..>2439
                     /locus_tag="PK717_07120"
     CDS             2114..>2439
                     /locus_tag="PK717_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47466.1"
                     /translation="MTIGSKKAFVNGLEWPVDITPPFIVSKTGRTMVPFRFIGEHLGA
                     TVDWFPKDKAVETVTYKLNDVYIELFIGKKTAKKNGQQIELDQEPMIVNGSTMVPVRF
                     VTEQFGS"
BASE COUNT          702 a          512 c          623 g          602 t
ORIGIN      
        1 ataaacatca ttgagactct caaaaatgaa ggcaccgagg actctcaggc cagggcagaa
       61 aatgtcgaag aatttctcaa cctttcgtat gaatttggca agacgtcgga agattccagt
      121 cttgaagctt ttttgggcac cgttacgctg atgagtgaca tggacaatgt ggaggaaggc
      181 gagcaaaggg tcataatgac aacgatccac caggcaaaag gtttggaatt cccagtcgtt
      241 tttttgtgtg ggcttgaaga gggcactttc cctcatctga gaagtcttga tgaccaggaa
      301 tcggtagaag aagagcgaag attggcctat gtcgggatga cgagggccaa ggaagtgctt
      361 tttttgacca gggttataaa caggcagctt tggggtggaa caagactttg cgaggaatcc
      421 agattcttaa aggaaatagc ttgcccggag attgtctatg aaggcatcaa aaaacagacc
      481 cagacggcaa gagagcaaca gaggagtttt gtaaagaaat ctgcacaagt agtcatcaca
      541 cccaaaaagg aaccaattaa agtttctact ggtgaggttg ttgttcatca ggcgtggggt
      601 gatgggactg tgttgaatgt gtccggagac aatgccgaag ttgattttct ttctgttggc
      661 agaaagctcc tgaatcttcg ctatgctcca ataaagtcca agggagcttg acaaagacag
      721 cattttgctt agccttgcat gtatggggct aaatttgtga acctgccagt cccccatctt
      781 gtagtaagta actgtaagga ggaaacgtga aaaaagcctt gttgcttctt ttgtgcgtcg
      841 taaccctctt ctctggagtt gtttttgccg atggcgagtg gaagccatat ttggatatcc
      901 tcgtatggag caatccaccc gatcttggaa taaggctcca gaccgagatt gaaaggcatt
      961 ttccaaacat agcctcctat cttagtgttg tgactccggc atcactcagg gagccgctgc
     1021 aatataatgg ctacaacgtc atttgtctgc ttgggtgcgc aaaggctgga caggtccttg
     1081 atgtgccaga ggatgtggaa acactcattg aagacaggtg caagtttggt atttctggtc
     1141 ttatcatctc aggagatgtc atccatccgg actcaaacaa cccaatcctc cagaaacttg
     1201 ccggtgcaca gctaatggat gttgaaaaag ctggaagaac aacggtcagg gtcatcgata
     1261 taaccgaccc gattgtttcc gacgttgccc agtcttttga cgtgacgctt ggagacatca
     1321 gaaaatccgc cctctatccg ggcacaaaat cgattgcagt aacaaattcg ggcgttcccg
     1381 tggttttcaa gaggaacata acccagtcaa aagcttgcta catagctctt ggccagaatg
     1441 gccaggctgt tgaaaatatt gaagtttcaa gattgtttgc caatgctctc tacgattgca
     1501 tgggaactgg gtggctccag ccttacgaag caccgacgaa tgtgaaggca ataataggcg
     1561 acgagaaggt gaaaatcacc tgggacaagc ccaaaaagat ggaaggtatt gcaggttaca
     1621 gagttttcag ggaatctgag aatgagaaat ccaaaacgat ccatgatttt gctctggata
     1681 ccgaaacgct gttttttgaa gattcctcgg ttaccaatgg caaactttat acctacaagg
     1741 tttgctcgat cggaaatgcc gacaagattg tagcctgctc gcatcaggtc caggcaaggc
     1801 cgggtaagat acaggtcacg ttaccaggtt ggcctgcaca gggtcagcct gtccaggtta
     1861 ttggctcaaa gcacatcgtc aggggaaaga cccgccctgg ttcaaaggtt attgtcaaat
     1921 ggaagctcat tccatcggac ttgagggtga aagtaagggt gaagcagacc aaaacggaaa
     1981 ttttgagata ccgattgatc ttaccccagg ccagaaagtt gaatattaca ttattgttga
     2041 aaacgagctt ggccagcagg aaaaattcgg cccatatatt gtggattgca aggcaaacaa
     2101 gatcgtcatt tcaatgacta ttggatcaaa gaaggccttc gtaaacggtc ttgagtggcc
     2161 ggtcgatata acaccgccat ttattgtctc gaaaactggc aggacgatgg tgcctttcag
     2221 gttcattggc gaacaccttg gcgcaacagt cgactggttc ccgaaggaca aagcggttga
     2281 aacggtcaca tataaactga atgacgtcta tatcgaactt tttattggta aaaagaccgc
     2341 caagaaaaat ggccagcaaa tcgagcttga ccaagagcca atgatcgtca acggaagcac
     2401 gatggtcccc gtaagatttg ttaccgaaca gtttggctc
//
LOCUS       DAOSRI010000252         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_39594_length_2426_cov_4.156474, whole genome shotgun sequence.
ACCESSION   DAOSRI010000252 DAOSRI010000000
VERSION     DAOSRI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39594_length_2426_cov_4.156474"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK717_07125"
     CDS             complement(<1..237)
                     /locus_tag="PK717_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47467.1"
                     /translation="MVLYEEKYFVDKRLPMLVLMIILAILVSFMLWLNIVDSSDHSLG
                     FSIIMELTMISGVLVTLAETRFTFRIKEDVLSFGF"
     gene            complement(282..782)
                     /locus_tag="PK717_07130"
     CDS             complement(282..782)
                     /locus_tag="PK717_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47468.1"
                     /translation="MVLYEEKYTVKTIVWVSIIAGFIIIASSVAVWFSVPKTEELWPI
                     ILINALAVLPAIFLLSVMTRFMVRIDEKTLSFGYPPIIVRIPLEQIGNVTFTKVGFLS
                     SGGIGIRLSSGGGWNYVTFWGDLARVEWSNGRRHGFSVKNPEEVKRILGKLLGKDKIF
                     EDYSKR"
     gene            927..>2426
                     /locus_tag="PK717_07135"
     CDS             927..>2426
                     /locus_tag="PK717_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47469.1"
                     /translation="MRTSFKKVLLVVLALAILASSAIVAKPSKAENVEIFGVASNSFG
                     YYIYVADSIHNRVIKYDLMGNYLADFIKTDKKGPCPNWDYKGLIAVSVCRITDVFAVT
                     DHILKKCFTFNPDSVPHHPIGDPSKNELVNPWHASETEIADDIGNYVIDRDGNKFCRF
                     DPPAIDFDKQAVECDVYGRMRFSVPDGYKSGAAPAGSGSGQFRNPEGIAVDRRGYVLV
                     ADTGNNRIQKFRRSGEFVMEWGEKGDGPKQFNKPVYIIPDYNDDSPNRYYICDQGNNR
                     IQVFDEYGKYLDTIQPMVDGKPLFNNIVACAIDMDYNIWVADAAEDGQRIYKLASVDS
                     PKKYQLLLTLNDPMNPPPIYIHVIRNQLKKYFASVDETKVSIKPYSSIINGRTMVPLR
                     WIAENVLTNKDPKKGVTYECKVDWNDKTRCATFTLDKIVFSDKLVYPKTVVTVCLNNP
                     VGSINGKPAPIDSTNPKVKPENVNDRTMVPARFIEEAFGGAYLAWGFIVT"
BASE COUNT          722 a          542 c          496 g          666 t
ORIGIN      
        1 aaaaccaaat gataaaacat cttctttgat cctaaaggtg aatctggttt ctgctagtgt
       61 aactagcact ccagaaatca tcgttagttc cattattatg ctaaatccta acgagtgatc
      121 cgatgaatcg acaatattta gccataacat gaaactaacg agtattgcaa gaataatcat
      181 taaaaccaac attggtaatc ttttgtccac aaaatatttt tcttcataaa gcaccatatt
      241 ggcctcctcc catgattttt tctttttatt attatgaaaa actatctttt tgaatagtcc
      301 tcaaaaatct tgtcttttcc aagcaactta cccaggattc tctttacttc ttcggggttt
      361 ttcaccgaga atccatgcct tcttccattg ctccattcaa ctcttgccag atcgccccag
      421 aatgttacat agttccatcc gccacccgag gagagcctta tgcctattcc gcctgatgac
      481 aggaaaccaa cttttgtgaa tgttacgtta cctatttgtt caagcggtat cctgacgatg
      541 atgggtggat aaccgaatga aagggttttt tcgtcaatcc tcaccataaa tcttgtcatg
      601 acagaaagca ggaaaattgc tggtaacacg gccagagcat ttatcaggat aattggccag
      661 agctcttctg ttttcggaac gctgaaccag acagcaacac tagaggcgat aattataaat
      721 cctgcgatta ttgaaaccca gacgatcgtt ttcactgtat atttctcttc gtaaagtacc
      781 ataaataaaa tctccttttc tccagggtaa atcctagaga gacattattg gacacactct
      841 gcgtgaaatt gtttttttgc agtggcctaa ttgattttaa tcattgcgca aattagacta
      901 aatttgaatc cttactggag gtgcttatga ggacaagttt taagaaagtg ctacttgttg
      961 tactggcttt agctattttg gcttccagcg ccattgttgc aaagccatcc aaagccgaaa
     1021 acgttgagat attcggcgtt gcatcaaaca gctttggtta ttacatatat gttgccgact
     1081 cgatccacaa cagggtcata aagtacgacc tgatgggcaa ctacctcgcc gatttcatca
     1141 agacagacaa aaaaggccca tgtccgaact gggactacaa aggcctcata gcagtttcag
     1201 tgtgccgcat tactgacgtt tttgcagtca ctgaccacat tctcaaaaaa tgcttcacct
     1261 tcaatccgga ctcagttcct caccatccaa ttggagatcc atccaagaat gagcttgtca
     1321 atccatggca tgcatcagaa actgaaatag cagatgatat tggaaactat gttatcgaca
     1381 gggatggaaa caagttctgc aggttcgacc cgccggccat tgattttgat aaacaggcag
     1441 tcgagtgcga tgtttatggc aggatgaggt tctctgttcc agatggttac aagagcggtg
     1501 ctgctcctgc tggcagtggc agtggacagt tcaggaaccc agaaggtata gctgttgacc
     1561 gtaggggata tgtccttgta gctgacactg gcaacaacag gatccagaag ttcagaagat
     1621 caggtgaatt cgtaatggaa tggggcgaga agggtgatgg cccgaagcag ttcaacaagc
     1681 cagtttatat cattccagat tacaatgatg actcaccaaa cagatattac atctgtgacc
     1741 agggcaataa cagaatccag gtttttgatg aatatggcaa gtatcttgat accatccagc
     1801 cgatggttga tggaaagcca cttttcaaca atattgtcgc ctgtgcgatc gacatggatt
     1861 acaacatctg ggtagcagat gctgcagaag atggtcagcg catctacaag ctcgcgagcg
     1921 ttgattcacc aaagaagtat cagcttctcc tcacactcaa tgacccgatg aacccgccac
     1981 caatatatat ccatgtgatt agaaaccagc tcaagaaata ctttgcctct gtcgacgaaa
     2041 caaaagtctc gataaagcca tattcttcaa taatcaatgg cagaacaatg gtcccgcttc
     2101 gctggattgc tgaaaacgtc ctcacaaaca aggacccaaa aaagggtgta acctacgagt
     2161 gcaaagttga ttggaatgac aaaaccagat gtgcaacatt cactctggac aagattgttt
     2221 tctccgacaa gcttgtctat ccgaaaacag ttgtcacagt ttgccttaac aatccagtcg
     2281 gctcgatcaa tgggaaacca gcacctattg attcaacgaa tccaaaggta aagccagaaa
     2341 atgttaatga tagaacgatg gttccggcgc gtttcattga agaagccttt ggcggcgcct
     2401 acctggcctg gggctttatc gtaacg
//
LOCUS       DAOSRI010000253         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_39879_length_2414_cov_3.357355, whole genome shotgun sequence.
ACCESSION   DAOSRI010000253 DAOSRI010000000
VERSION     DAOSRI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39879_length_2414_cov_3.357355"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK717_07140"
     CDS             <1..1063
                     /locus_tag="PK717_07140"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOR47470.1"
                     /translation="MPTITHLLDRGEKLIICSHQGRPDGKYVEQYSLKPIAEHLRELI
                     NKPIKMAPDCVGCQVSSMVDSLMPGEALVLENLRFHKEEEECDESFSKELASLGEIYV
                     NDAFGTCHRKHASIYGVPLILKPAVAGLLLVKELKYLGDTTENPKRPFAVVFGGAKVS
                     DKIKVVYNLEKIADLIVIGGGMSFTFMASQGIGIGTSLVQKDMIDSCRTFLEECRKKG
                     KKVLLPVDNIVADNYKNPTDIKISANGIPDGMEGVDIGPKTSKMFCDELTKCETVVWN
                     GPLGVFEDERFAEGTKTVMKCVAGGVKVSVIGGGDSAAAAAKFGFTNQFTHVSTGGGA
                     SLEFLEGKELPGIKVLDDA"
     gene            1008..>2414
                     /locus_tag="PK717_07145"
     CDS             1008..>2414
                     /locus_tag="PK717_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47471.1"
                     /translation="MNSLKGRSSQALKFWTTLNLSALLVFTCAVIPVRNAEKASSLGI
                     TSALIIPCSQMEGLYLDQAQETWVEGCSRRSTGFFEYKIRFDGEGPILLEVETSGSIV
                     IEASLNGEPLKQYFESGQIDEDISVKMKRQLRLDASGGSPLRVKFSKKKGSSKPFALW
                     KLYVRRGVEIYPGTPTEQAYLDNAGSSILLPGGGRGLSKGDEVKYKVNQAEAGPLGVV
                     IESIGDMKVLLASGGAPEGTELMKSGEFTYGFMTANDLNSSIVLRATGELKIIRVSIY
                     FGLSCMDFQTELSKMVFPGFGKEAILSADEPIVFKLPPHADSIFWCQAENIASIKAEG
                     LEIYSLSGCLFTKVPQSLNEVKLQGSGKLYSWGIDQDPDEDSLPSSFEMAYGTDADRA
                     FTDFDEQNDYADIMPLDTDNDGLDDDVERFLGINPSYYDSNSDGYPDSLGLSTPKLSP
                     FCANITRKPWPGVLNQDVY"
BASE COUNT          639 a          513 c          622 g          640 t
ORIGIN      
        1 catgcctacg attactcacc tattagatcg tggcgaaaag cttattattt gctcacatca
       61 ggggagacct gacggcaaat atgttgagca atactcatta aagcccatcg cagaacattt
      121 gagggaattg attaacaaac ccataaagat ggcacctgac tgcgttggct gccaggtcag
      181 ttcgatggtt gactcgctca tgccaggaga ggcattggtg ctggaaaatc tcaggttcca
      241 caaagaagaa gaggaatgtg atgaatcgtt ctccaaggag ctcgcctcac ttggtgagat
      301 ctatgtgaat gacgcatttg gcacatgcca cagaaagcac gccagcatct atggtgttcc
      361 ccttattctg aaaccggccg ttgcaggatt acttctggtg aaagaactca agtatcttgg
      421 cgacaccacg gagaatccca aaaggccgtt cgctgttgtt tttggcgggg ccaaggtttc
      481 tgacaaaata aaggtcgttt acaatcttga aaaaatcgct gacttgatag tcattggtgg
      541 tgggatgtcg tttactttca tggcctcgca gggcattggt attggtacat cgcttgtcca
      601 gaaggatatg atagacagct gcaggacatt ccttgaggaa tgcagaaaaa aaggaaaaaa
      661 agtcttgctg cctgttgaca atattgttgc ggacaattac aaaaacccta cagacataaa
      721 aatttctgca aatggcattc ccgatggaat ggaaggcgtt gacattggcc cgaagacctc
      781 aaaaatgttc tgcgatgaac ttacaaaatg tgaaacagtc gtgtggaatg gccctttagg
      841 cgtgttcgag gacgaacgtt ttgccgaggg caccaagacc gtcatgaagt gcgtggctgg
      901 cggagtcaag gtgtctgtga tcggcggagg tgattctgcg gccgctgcgg caaagttcgg
      961 attcacgaac cagttcacgc acgtatcaac aggaggaggg gcatcgcttg aattccttga
     1021 agggaaggag ctcccaggca ttaaagttct ggacgacgct taatctttcg gcccttttag
     1081 tttttacctg tgccgttatt ccggtcagga acgctgaaaa agcgtcctcg cttggcatta
     1141 catctgcact tataattcca tgctcccaaa tggagggtct ctatcttgac caggcacagg
     1201 agacatgggt ggagggttgt tcaaggagat caacgggctt tttcgaatac aagataaggt
     1261 ttgatggtga aggcccaata ctgctggaag tggaaacttc cggcagtatt gttattgagg
     1321 cctccctcaa tggcgagccc ctaaaacaat actttgaatc aggtcagatt gatgaggaca
     1381 tctcagtaaa gatgaagcgc cagttgaggc ttgatgcttc tggcggttcg cccttgcgtg
     1441 tgaagttctc aaagaagaaa ggctcttcaa aaccttttgc cctctggaaa ctatatgtcc
     1501 ggagaggtgt ggagatttac cctggaaccc ccacagaaca ggcatatctg gacaacgccg
     1561 gttcgtcgat tttgcttcct ggtggtggca gggggttatc caagggtgat gaagtaaagt
     1621 ataaagtgaa ccaagctgaa gctggtcctc tcggtgttgt aattgagagc atcggagaca
     1681 tgaaagtctt gctggcttca gggggtgctc ctgagggtac cgagcttatg aaatctggtg
     1741 agtttaccta tggtttcatg acggccaacg atctgaattc ttcgattgtt ctaagggcaa
     1801 ctggtgaact caagattata agggtttcta tctattttgg gctctcttgc atggattttc
     1861 aaactgaact ttcaaaaatg gtttttcctg gttttggcaa agaagctatt ttaagtgccg
     1921 atgagcctat tgttttcaaa ctgccaccac atgctgattc cattttctgg tgccaggcgg
     1981 aaaatattgc ctcaataaaa gctgaagggc tcgaaatata cagtctttca gggtgcttgt
     2041 ttacaaaagt tccacaatct ttgaacgaag taaaactaca gggctctggc aagctgtatt
     2101 cctggggcat tgatcaggac cctgatgagg acagtttacc ctcaagtttc gagatggcgt
     2161 atggcactga tgctgacagg gcgtttaccg attttgacga gcagaacgac tatgcggata
     2221 taatgcctct cgataccgac aatgatgggt tggatgatga tgtggaacgc ttccttggga
     2281 tcaacccttc atattatgat tccaattctg atggatatcc tgactcgctt ggactctcga
     2341 ccccgaaact ctcgccattc tgcgctaaca taaccagaaa gccatggcca ggagtcctaa
     2401 accaggatgt ttat
//
LOCUS       DAOSRI010000254         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_40242_length_2399_cov_4.384386, whole genome shotgun sequence.
ACCESSION   DAOSRI010000254 DAOSRI010000000
VERSION     DAOSRI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40242_length_2399_cov_4.384386"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK717_07150"
     CDS             <1..1168
                     /locus_tag="PK717_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47472.1"
                     /translation="GKAEWFVTKPKLSIVAQSSCEEKPTVFVDIDGDQVKYSGEFSLE
                     DGRHFVTCYCVDEFGNKSETQTVLINIDTKPPQFDPPSGKIYTRSDSIRESLAIADDN
                     DVELYPTDTTGVLFSKGLTSVWWVEYVSSGQNCIKAQAVAEDEAGNKAVWDREVCFDW
                     TPPKLELPEKLEADKPEYSIAGKVSDNSPVVVTVNGVALEVKEDGSFSYAGIAKNGPQ
                     NIQFVAKDAAGNQTIANVLVIGNLRRSVVISIDSKEAFVNGKVVTLPVAPYLEKGTTM
                     VPLRLIGEGLSAKVDFNQENKSIKIVLGQIQVELVLGNSTALVNGQPVTLPVPPGTKK
                     GTTFVPLRFVAESLGAQLMVSGKKITISYIITPDAGNPLFFSSPRLPIFLCSIR"
     gene            1290..>2399
                     /locus_tag="PK717_07155"
     CDS             1290..>2399
                     /locus_tag="PK717_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47473.1"
                     /translation="MVPARNESLVIARTIENLTKLDYDMNSLEVIVVTDQKERREHEN
                     FKPVLIGELHNLLSDPDFVPKEIKTSGFFLSYVVTKLPYGQIMFQCMDIAPKSDRALL
                     RGKIERAIFEFCNTIISGRPADLSTVYYGLRRAMPGIEDERLSQVTTEFLLLSRLAIG
                     KLLERNPQYISALKRIEIISTMSGGLPPMRTENLRLAELDRLLYREEHPTIEKLGELY
                     DSFFLTTQQVVEDCIVANNGAGFRLKHSEVPFDFDGEMDGKCTGEPIKSTKGRALNWA
                     FSDVDPQTDMVVFYDAESHPDHDVMLHAGRRLISKDPPEIMQGPLFQVRNYFRMGAIS
                     RIGGLYKAIAHDWYLPLLIKKLPFVGGTNLFISWKL"
BASE COUNT          667 a          532 c          578 g          622 t
ORIGIN      
        1 tggcaaggcc gaatggtttg tcaccaagcc aaagctttca attgttgccc agtcttcttg
       61 cgaggagaag ccgacagttt ttgtagatat tgatggagat caggtgaagt attctggtga
      121 attctcgctt gaggacggac ggcattttgt aacgtgttat tgtgttgatg agtttggaaa
      181 caaatccgag acacaaacag tccttatcaa tatcgatacc aagccgccac agtttgaccc
      241 cccatctggc aagatctaca ccaggtccga ttcgataagg gaatcacttg caatagccga
      301 tgacaatgat gtagagctat atccgacaga caccacaggg gttttgttct caaaagggct
      361 cacgtctgtc tggtgggtgg aatatgtttc atccggtcag aattgcatca aggcccaggc
      421 agtggctgaa gatgaagcag ggaacaaggc tgtttgggac agagaagtgt gctttgactg
      481 gacgccacca aaattggaat tgccagaaaa gcttgaagca gacaaaccag agtattcgat
      541 tgctggaaaa gtatccgaca actcgccagt tgtagtgact gtaaatggcg tggctcttga
      601 ggttaaagaa gatggatctt tctcctatgc aggcattgcc aaaaatggcc cgcagaacat
      661 ccagtttgtg gcaaaagatg cagcgggaaa ccagactatt gcaaatgttc ttgtgattgg
      721 aaacctcagg agatcggtcg tcatttcaat tgacagcaag gaagcttttg taaacgggaa
      781 ggtcgttacg cttccggttg ccccatacct tgaaaaggga accaccatgg tgccgctcag
      841 actcattggc gagggtctgt ctgcaaaggt tgatttcaac caggagaaca aatctatcaa
      901 gattgtcctt ggacaaatac aggttgagct tgtccttggg aacagcacgg cattggtaaa
      961 tggccaacct gtaacactac cagttccacc aggaacaaaa aaaggtacta cctttgtccc
     1021 attgaggttt gttgcggaat ctcttggtgc ccagctcatg gtctctggca aaaaaataac
     1081 tatcagctat ataattaccc ctgatgctgg aaatcctcta ttctttagct ctcctcgcct
     1141 acctatattc ctttgttcta ttcgttaggt tttttgtatg gcgatttcag gcaaataggg
     1201 atttttggaa cagaaaagta agtatatccc ttgaagacct gaggttcctt gcaaaaagag
     1261 agggtgtggg cctgcctttc tttactgtaa tggttccagc tagaaacgaa tcgcttgtca
     1321 tagccaggac aattgaaaat ctcacaaagc ttgattatga catgaattca cttgaagtta
     1381 tcgttgtgac tgaccagaaa gagcgccggg agcacgaaaa tttcaagcca gtgctcatcg
     1441 gagaactcca caaccttctt tctgatcctg attttgttcc aaaagagatc aaaacatcag
     1501 ggttttttct ttcgtatgta gtcacaaaac ttccttacgg ccagataatg ttccagtgca
     1561 tggacattgc gccaaaatct gacagggcat tgctcagggg caagatagaa agggccatat
     1621 ttgagttttg caacaccata atttctggca ggcctgcaga cctgagtaca gtctattacg
     1681 gtttgcgaag ggcaatgcct ggaattgaag acgagagact ttcacaggta acaacagagt
     1741 tcctcctgct ctccaggctt gccattggca agctcttgga gagaaacccc cagtatattt
     1801 ctgcactcaa aagaatagaa atcatttcta caatgtctgg cggactcccg ccaatgagga
     1861 ccgaaaacct caggctggcc gaactggaca ggcttttgta cagggaagag catcccacaa
     1921 tagaaaaact tggtgaactt tatgattctt tcttcctgac aacccagcag gtagttgagg
     1981 attgtattgt tgccaataac ggtgcagggt tcaggctcaa gcactccgaa gtcccatttg
     2041 attttgatgg cgagatggat ggaaaatgca ctggggaacc gatcaaatcc acaaaaggca
     2101 gggcactcaa ctgggcgttt agtgatgttg acccccagac tgacatggtt gttttttatg
     2161 atgcagaatc gcacccggac catgatgtga tgcttcatgc tggtcgccgc ctcatctcga
     2221 aagacccgcc agaaataatg caggggccgc ttttccaggt tagaaactac ttcaggatgg
     2281 gtgccatctc aaggattgga ggcctctaca aggccatcgc ccatgactgg tacctgccac
     2341 ttctcataaa aaaactcccc tttgtaggag gaaccaatct tttcatttcg tggaagctg
//
LOCUS       DAOSRI010000255         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_40293_length_2397_cov_3.187447, whole genome shotgun sequence.
ACCESSION   DAOSRI010000255 DAOSRI010000000
VERSION     DAOSRI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40293_length_2397_cov_3.187447"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK717_07160"
     CDS             <1..290
                     /locus_tag="PK717_07160"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOR47474.1"
                     /translation="GLLCQGVFGGQTNGEMFTFSSMVTYKIQNGKIGQMCQGSVLQGN
                     VFETLRAIDMVGKDFVQPDSAGGCGKGGQMPLPTSHGSPSIRIQKIVVSGK"
     gene            299..1636
                     /locus_tag="PK717_07165"
     CDS             299..1636
                     /locus_tag="PK717_07165"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR47475.1"
                     /translation="MIEKIIEQAKKHFQSVEVIHYNRTTTPVSFESGRLKEISETQRE
                     GYAMRAVKDGKLAFSTTTKPGDVEGLINQALETIKYSPVMDFEFASNASFASPIITSK
                     ETKELTIQQMVDMGKETIGAISGLHPKARAHAGVTKSHFKTEMATSNGFKGSYEKDTF
                     SWGCYIDLTDGDNMLGCGEGFIGIKDEAKKKELVEKAIRNFKYGQKNVKTNSGKYPVL
                     FTPNAFLHAINILLASVGGKSVERKVSPWTDKLGTKVASNMFTLIDDGRMDLRNHSCP
                     YDDEGTPTQRTSLIENGVLKNFLLDKRTAKKLSMASTGNAFKKGGIFNPPPEIDNAPV
                     ITQSTIVMQPGSKPSNEILKGIKKGFYFDGLIGTMMGNPLTGQLAGNVDLGFLVENGE
                     IVGRIKDAMVSVNLFEALNNNIAAISSDVEISGFNGEMVLPYILLDNCTVAIG"
     gene            1738..2361
                     /locus_tag="PK717_07170"
     CDS             1738..2361
                     /locus_tag="PK717_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47476.1"
                     /translation="MIQITSTIYGNINGAQFLGNHSGTANPSSGTSSSTIQYTAFPLD
                     FTPLACRSWTCKHHLSICMETDGAINLLNFTGGNYDTDETVFYPGGGQIITTSQVRLV
                     NPNLETCQQTFSGNVQITKEILSLEPFTETLMPDGPGKIIKQGSRRIVFSDNSSIVLT
                     WSGVILFVDQSLVFPFAQAIDYTYSSASFDRQSLLLTKELVAVARRL"
BASE COUNT          755 a          513 c          541 g          588 t
ORIGIN      
        1 acggactgct ctgccagggc gtctttggcg gccagaccaa cggggagatg ttcaccttct
       61 cttcaatggt cacatacaaa atacaaaatg gaaagattgg gcagatgtgc cagggaagcg
      121 ttcttcaagg caacgtcttc gagacactta gagcaatcga catggtaggc aaggatttcg
      181 tacaacctga cagtgccggt ggctgtggaa agggtggcca gatgcctctt ccaacctcgc
      241 atggttcgcc atcaatccgc atccagaaga ttgttgtttc gggaaagtag gtgcaagcat
      301 gatagaaaaa ataattgagc aggcaaagaa acactttcag agcgtcgagg tcatccacta
      361 caacagaaca acaacaccgg taagttttga atccgggagg ctcaaagaga tttcagaaac
      421 ccaaagggaa ggttatgcaa tgcgtgccgt gaaggatggc aagctggctt tctcgaccac
      481 aacaaagcca ggtgatgttg aaggcttaat aaatcaggcg cttgaaacaa taaaatactc
      541 tccggtgatg gactttgagt ttgcttcaaa tgctagtttt gcatccccga tcataacaag
      601 caaagagacc aaagagctta caattcagca aatggtggac atgggcaaag aaacaatcgg
      661 cgcaatatct ggccttcacc caaaagccag agcacacgct ggtgtaacaa aaagtcattt
      721 caagactgaa atggcaacaa gcaatggatt caaaggctct tacgagaagg acaccttttc
      781 ctggggatgc tacatagacc tgactgatgg cgacaatatg ctcggttgtg gtgagggttt
      841 tataggcatc aaggatgaag ccaaaaagaa agaactggtt gaaaaagcaa tccggaattt
      901 taaatatggg cagaaaaatg taaaaactaa ttccggcaaa tatccagtgt tgtttacacc
      961 aaacgcattt ctccatgcca taaatattct tttggcatcc gttggtggca aaagtgttga
     1021 aagaaaagtc agtccatgga cggacaagct tgggacaaaa gtagcttcta atatgttcac
     1081 gctcattgat gacggtagga tggaccttag gaaccattct tgcccttatg atgatgaagg
     1141 caccccaacc cagcgcacct cactcattga aaatggtgta ttaaaaaact tccttctgga
     1201 caaaagaact gcaaaaaagt tgtcaatggc atcaactggc aacgcattca agaaaggcgg
     1261 cattttcaac cctccaccag aaattgacaa cgctccggtc atcactcaat ccacaatagt
     1321 catgcaacca ggtagcaagc cctccaatga aatcctgaaa gggatcaaaa agggcttcta
     1381 ttttgatggg ctcatcggga ccatgatggg caacccactg acaggccagc ttgctggaaa
     1441 tgttgacctc gggttcctgg tagaaaacgg cgagatcgtt ggcagaataa aagatgccat
     1501 ggtatcagtc aatctctttg aagcattaaa caataacata gctgcaataa gctcggatgt
     1561 ggaaatttca gggtttaatg gcgaaatggt actgccatat attctacttg acaactgcac
     1621 tgtagccatt ggataataga acctgaccag gtcggctaag atgccgatct ggtcttttct
     1681 tttttaagtg ttttgataaa ttgtcttgtt aaaaccacgt aagacacggg aggtaacgtg
     1741 attcagataa cgagcacaat ttacgggaac atcaacggtg cccaattttt aggaaaccac
     1801 agtggcacag caaatccaag tagcggcaca tcgtcctcaa caatacagta caccgcattt
     1861 cctttggatt tcactcctct tgcctgtaga tcatggacat gcaagcacca tctttcgatt
     1921 tgtatggaaa ctgatggcgc aatcaatctt ctaaacttta ctggaggcaa ttacgacacc
     1981 gatgaaaccg tcttttaccc gggtggtgga caaatcataa caacaagtca ggttagactt
     2041 gtaaatccca acctcgaaac atgccagcaa accttcagtg gtaatgtcca gatcacaaaa
     2101 gaaattttat cattggaacc ttttacagag actttgatgc cagatgggcc aggaaaaatc
     2161 atcaaacaag gctcaagaag aatcgttttt tcagacaact catcgattgt tctaacatgg
     2221 tccggggtta ttctttttgt tgatcaatca ttggtgtttc cttttgcaca agcaatcgat
     2281 tatacttatt caagtgcttc tttcgatagg cagagtcttc tgcttacaaa agagctcgtt
     2341 gctgtagcga ggagattatg acgggtgaaa ataaggtaat catcaaaatg gtagacc
//
LOCUS       DAOSRI010000256         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_40384_length_2393_cov_3.575278, whole genome shotgun sequence.
ACCESSION   DAOSRI010000256 DAOSRI010000000
VERSION     DAOSRI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40384_length_2393_cov_3.575278"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK717_07175"
     CDS             <1..717
                     /locus_tag="PK717_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR47477.1"
                     /translation="AAIGIIGGADGPTAIFIAQKMAPQILGAIAVSAYSYMSLVPIIL
                     PPVIRCMTTKKERTTRMELAKPVSKTTKMLFPIVVTLVVGILLPSSVQLIGMLMAGNF
                     IKECGLVERLSNTLQNELVNINTVFLGLTVGSTMAAQQFLTPATLKIILLGFLAFAFS
                     AAGGVALGKLMYLLSGGKINPMIGAAGVSAVPMSARTVQKEGQREDPGNFLLMPAMGP
                     NVAGVLGTAVAAAALIALLS"
     gene            837..1637
                     /locus_tag="PK717_07180"
     CDS             837..1637
                     /locus_tag="PK717_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR47478.1"
                     /translation="MKKPTLTQTMRYVTQWGMLLYLASISTTHMYRWPGTSAGPLDAY
                     CPMGGLETLLPFVTSGQIVRGTNFNAILLLGALTILVLIFSGGFCGYMCPFGTVLEWV
                     YKIRKLFWKKTVRVPNKLSSALSHARYGVLILIVLITFAIDTELHFAHIDPYRAFFHF
                     GREMTTELWVVLGVVFGLSIVLERGFCAYLCPLGGYVGLVSLAGFTKIKNNGEKCIDC
                     KLCDRACPVNLTPSGKLEHHRCIMCFRCVDACVGTKSLRVSLADKEWR"
     gene            1733..>2393
                     /locus_tag="PK717_07185"
     CDS             1733..>2393
                     /locus_tag="PK717_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47479.1"
                     /translation="MNKKARIIIETVAVVLALIVLVFFATFNFDLFAINTFGKKSDMT
                     FIINRTSHLGNTSTEKEIKEIYKRNYPYYFYNNKISKLKIEDDGYITKELLDAITNEN
                     NYLEFPGEVISQLGHYFLVKKDDGNYIYEIDEQDYKTTISKYVKTPCNRLVCINDYLC
                     CATPNNDMIYMYPGLDLRSTPKILNFKKPSFISGPEEQSISYELYVFDGQFLTHYNDD
                     GS"
BASE COUNT          676 a          575 c          510 g          632 t
ORIGIN      
        1 gctgccattg gaatcattgg tggagccgat ggcccgaccg caattttcat cgcgcagaaa
       61 atggcacccc agattctcgg tgcgatagca gtatcggctt attcctacat gtcactggtt
      121 cccataatat tgcctcctgt catcaggtgt atgactacca aaaaagaacg tacaacacgt
      181 atggaactag caaaaccagt ctcgaaaaca accaagatgc tgttcccaat tgtcgtcaca
      241 ctcgttgttg gaatccttct cccatcgtct gtacagctca tcggaatgct catggccggc
      301 aatttcataa aggaatgcgg gcttgtcgaa aggctttcaa ataccctgca gaacgagctt
      361 gtaaatatca acacagtatt tcttggactg actgttggct ccacaatggc cgcccagcaa
      421 ttcctcaccc cggcgaccct aaaaatcatc cttctcggct tcctcgcctt tgccttctcg
      481 gctgcaggcg gagttgccct tggaaaactg atgtacctgc tttctggcgg caagatcaac
      541 ccaatgatcg gcgcggcagg tgtttcagcc gtcccgatga gcgcaagaac tgttcaaaaa
      601 gaaggccaga gggaagaccc aggcaacttc ctcctcatgc cagcgatggg cccaaacgtt
      661 gccggcgtcc tcggaacagc agttgctgca gcagctctta ttgcacttct ctcctaagcg
      721 acaacccaaa tataactttc aaaaggcctg catgattgca ggcctttttt gtttgccttt
      781 gccaacaatg cacctttaac tgagattttg aaattatgtt gctatgttgt atccgtatga
      841 aaaagccaac tttgacccag acaatgagat atgtgaccca gtggggaatg ctcctttacc
      901 ttgccagcat atcaacgacc cacatgtaca gatggcccgg cacctctgca ggtccccttg
      961 atgcctactg cccgatgggt ggccttgaaa cactgctccc atttgtaaca tcaggccaga
     1021 tagtcagggg aaccaatttc aacgccatcc tcctgcttgg tgccctaacc atacttgttc
     1081 tcatcttctc cggtggtttt tgtggctaca tgtgcccgtt tggcaccgtg cttgaatggg
     1141 tctacaaaat aagaaaactg ttctggaaaa aaactgtaag agtaccaaac aagctcagct
     1201 ctgccctgtc tcatgccaga tacggagtcc tcatcctcat cgtactaatc acctttgcaa
     1261 ttgacacaga gctccatttc gcccacatcg atccctatag ggcatttttc cattttggcc
     1321 gtgagatgac cacagagctg tgggttgtac ttggcgttgt ttttggcctg tcaatcgtcc
     1381 ttgagagggg tttttgcgct tacctctgcc cacttggcgg atatgttggc ctggtttcac
     1441 tggccgggtt cacaaaaatc aaaaacaatg gcgaaaaatg cattgattgc aagctgtgcg
     1501 acagggcatg cccggtaaac ctcacgccat cgggaaaact agaacaccac aggtgcataa
     1561 tgtgcttcag atgtgtggat gcctgcgttg gcacaaaaag ccttcgcgtg tcgcttgccg
     1621 acaaggaatg gagataatta ttaatcgaag attttcgcaa aataccaggg attggaaaag
     1681 gtggggaagg aatatatcat atttggagga atgcaaggaa cggggggctt aaatgaataa
     1741 aaaagcacgg atcataattg agacagtggc cgtggtattg gcgctcatcg tattggtatt
     1801 ttttgccaca ttcaacttcg atctcttcgc cattaatacc tttggaaaga aatctgacat
     1861 gaccttcatc atcaatcgaa ccagccacct tgggaatact agtacagaaa aagaaatcaa
     1921 ggaaatctat aaacgaaatt atccatatta tttttacaac aacaaaatct caaaactcaa
     1981 aatagaagat gatggttata taactaaaga acttctggat gccatcacaa acgaaaataa
     2041 ttaccttgaa tttccaggtg aagtaatatc acaattaggt cattatttcc tggtcaaaaa
     2101 agatgatggt aattatattt atgagataga cgagcaagac tataaaacta ctatttcaaa
     2161 atatgtaaaa actccctgca accgtttggt gtgcattaat gattatttgt gttgtgcaac
     2221 accaaataat gacatgattt acatgtatcc agggttggat ctgagaagca ctccaaagat
     2281 actgaatttt aaaaaacctt cgttcatctc agggcctgaa gaacaatcca ttagctatga
     2341 actgtacgtc ttcgatggcc aattcctcac ccactacaat gatgatggct ctg
//
LOCUS       DAOSRI010000257         2378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_40718_length_2378_cov_3.685321, whole genome shotgun sequence.
ACCESSION   DAOSRI010000257 DAOSRI010000000
VERSION     DAOSRI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40718_length_2378_cov_3.685321"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK717_07190"
     CDS             complement(<1..541)
                     /locus_tag="PK717_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOR47480.1"
                     /translation="MKKNYLRIIILSVIALGLVAGATLTLVEYFKNKTTEDTKTVERP
                     VSQPKIKGPTGVVAKVGDIEVPLSVFSQLVNTTRQQALAEGTDIDDSKNASMLQAARE
                     DALNQLVEYAVYENYAKKNGILPTSKEIDAAIASEIDARVHQAGSRQSLEKQLTDLGG
                     IEGLKETIKGDPIFLRQVIR"
     gene            621..>2378
                     /locus_tag="PK717_07195"
     CDS             621..>2378
                     /locus_tag="PK717_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47481.1"
                     /translation="MDTRYYKGDQNLSWDALLVAKALLDLAGSATFFIQDEKLGTADY
                     KLEYNSKFALTTDYCGKLTGAVWIHQDTSSGPFYALPVEPYIYKRFGFSLKGAIDSYE
                     RVAKLFGMEKELGDLGINLRPGQMLRGLTEGERYVGIVPTTQDEKSISSYRLADDSLL
                     ERYYFMFLACYRGALQSVSREKWPDVKVRAKKILGMTKDLLSSKPEATISDLQETFWR
                     FGFSEFFNIAPPKIMRASEVFDVKGEINHIVLLTLLRNTKAFVESYNEALNKTHLPLK
                     RLKLRDGVMELPYYIECEYEGRLVRWHIRAHFGEKLILKMTYRNAEPKTMTISNPPSL
                     DELKNGLVGLFGCHTLIGKAGPLLAELSRPPKVISLPEQGSKYAPMVGHLTKELQSRG
                     INYPSGEFLRIGLRAIDTMELLGDEEIFLPPFLKAFWGEARAASWIARHWKEEATAAK
                     EMLESLHFDEGQLLTLAKYAIYEYEDALPNPVTPKMTKLGNIAGISKPLTARTYAKLK
                     ELIETREVLLSERREKMADFQKAEELLNVEMAIKLVSVGILKRFEQLTSLFYVNNRPY
                     AISFYLAFGPEIINRIAQGA"
BASE COUNT          645 a          499 c          567 g          667 t
ORIGIN      
        1 gtctgatcac ttgcctgaga aatattggat cacccttgat tgtttccttg agtccttcga
       61 tcccgccgag atcggtgagc tgtttttcga gtgattgcct tgaacctgcc tgatggactc
      121 ttgcatctat ctccgaagca atagctgcgt caatctcttt tgatgttgga agaataccgt
      181 ttttctttgc gtagttttca taaacagcat attctacaag ctggttcagc gcgtcttccc
      241 tggctgcttg cagcattgaa gcgttttttg agtcatctat atctgttcct tcagcgaggg
      301 cctgttgtct ggtggtgttg accagctgtg aaaaaactga caatggcact tcaatgtctc
      361 cgacctttgc caccacacct gttgggcctt taatttttgg ctgggagaca ggtctttcca
      421 ctgtttttgt atcttctgtg gtcttgtttt taaagtattc caccagtgta agtgttgcac
      481 cggccacaag ccctaaggct atgacagaca aaatgattat cctgagatag ttctttttca
      541 cggaagtgat tttacattca ggataccggt tgtcaaaggg ctttatggaa actggcaaga
      601 ttttcagtta atatctgatt atggataccc gctattacaa aggagaccag aatctttcat
      661 gggacgctct tcttgtggca aaggcactgc ttgatctggc tggcagtgcg acatttttta
      721 tacaggacga aaagcttgga acggccgatt acaaattgga gtataacagt aaatttgctc
      781 tcacgaccga ctattgcgga aagcttacgg gtgccgtatg gatccatcag gatacatctt
      841 caggcccatt ctatgcttta cctgtggagc cctatatcta taaaaggttt gggttttcac
      901 tcaagggggc gattgattca tatgaaagag tcgcaaaact ctttgggatg gaaaaagagc
      961 ttggcgatct cggcattaac ttgagacctg gccagatgct gagaggcctt actgaaggcg
     1021 aaagatatgt aggtattgtg ccaacaacac aggatgaaaa atccatatcg tcctacagac
     1081 tcgccgatga cagtctcctg gagagatatt attttatgtt ccttgcttgt tacaggggag
     1141 ccctccagtc ggtttcgaga gaaaaatggc ctgatgttaa agtaagggcc aagaagatac
     1201 ttggcatgac aaaagacctt ctttcatcaa aaccagaagc cacaatctcg gatttgcagg
     1261 agaccttctg gaggtttggt ttttcagagt ttttcaatat tgctccgcct aaaataatga
     1321 gagcatcgga ggtttttgat gtaaagggcg agataaacca tatcgtgctc ctcactcttc
     1381 taagaaacac caaagcattt gttgaaagct acaacgaggc gctcaataag acccaccttc
     1441 cattaaaaag gcttaagctt agggatggtg tgatggaact tccctattac attgaatgtg
     1501 agtatgaggg gcgtcttgtc agatggcaca tcagggcgca tttcggcgag aagcttattc
     1561 tgaaaatgac atatcgcaat gctgagccaa aaacaatgac catttcaaat cccccttcat
     1621 tagatgagct caaaaatggc ctcgttggac tttttggttg ccacaccctc attggcaagg
     1681 ctggcccgct ccttgctgaa ttgagcaggc caccaaaggt catttcgcta ccggagcaag
     1741 gttcgaagta tgccccgatg gttggccatc tcacaaaaga attacaatca agagggataa
     1801 attatcccag tggggaattt ttgaggatag ggttgcgtgc aattgacacg atggaactac
     1861 tcggtgatga agagattttt cttccacctt ttttgaaggc gttttgggga gaggcacgag
     1921 ctgcttcctg gattgccaga cactggaaag aggaggcaac ggcggcaaag gagatgcttg
     1981 agagcctcca ttttgacgaa gggcaattgc ttacgctggc aaaatatgcc atttatgaat
     2041 atgaagatgc cctgccgaat cctgttacgc caaagatgac aaaattgggt aatatagccg
     2101 ggatatcgaa gccccttaca gcgcgaacat atgcaaagct gaaagagttg attgaaacaa
     2161 gggaagttct cctctccgag cgtagggaaa aaatggccga ttttcagaaa gcagaagaac
     2221 ttcttaatgt tgaaatggca ataaagcttg tatcagttgg cattctgaaa aggtttgaac
     2281 aactgacctc gcttttttat gtaaacaaca ggccatatgc aatcagtttc tatcttgcat
     2341 ttggcccaga gatcataaac aggattgcac agggcgcc
//
LOCUS       DAOSRI010000258         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_40926_length_2369_cov_3.482714, whole genome shotgun sequence.
ACCESSION   DAOSRI010000258 DAOSRI010000000
VERSION     DAOSRI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40926_length_2369_cov_3.482714"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PK717_07200"
     CDS             <1..1499
                     /locus_tag="PK717_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOR47482.1"
                     /translation="NRQYELTGKAMIVAYSRPIAMRIYRKMLDMRPAWKDKLGVVMTS
                     GNDDPEDWHKIIGNKQHKAKLARQFKDPDDPFKIAIVVDMWLTGFDVPSLATMYVYKP
                     MSGHNLMQAIARVNRVYKDKEGGLIVDYVGIASALKQAMNDYTRRDRQNYGEQDIAKT
                     ALPKFVEKLEVCRNLFHGFDYSDFMTTQSDLVRAKTIAGGVNYLSAVEVKPTHALDVW
                     QGSSLRAASPEREEPSKKDLFIKEAMLLRQALSLCRSLLTEPQRIEAAYFEAVRTVLT
                     RLTSSGKPLSLKEINERINNLLKASIKSEGVINLFSSVDSGFSLFDPKFLEEIGKMKE
                     RNLAVELLRKLMAEQVAVYRKNNLVKSQKFSERLTATMNAYLNGLLTNEQVIAELIKM
                     AKEINNAKAEGEAMGLTEEELAFYDALTSPQAIKDFYENDVLVSMTRDLTEMLRKNRT
                     IDWQKKESARAKMRRMVKKLLRKYKYPPEGMEDAIATVIGQCEMWVDC"
     gene            1623..>2369
                     /locus_tag="PK717_07205"
     CDS             1623..>2369
                     /locus_tag="PK717_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47483.1"
                     /translation="MPNGMLNSVIKFDLHIHSEASKYKESKGIVDNSNKHNLATLLNK
                     LNEHDVALFSITDHNRFDPELYIEINKILSEKDNPYPNVKAVLAGVEFDVILDDGMDK
                     CHIIAIFETDNCIEKFSRIDTGIRTNLLKDAQEAYSRKDFENVLKEIGLNTILIASQR
                     KDIQNQSGKHNSLSDSAINVEQIIRMGYINALEFQKPKVEGIILNSLRKLSLPITLFS
                     GSDCHDWEHYPFHDSVNQNKEFHHSKAKMLP"
BASE COUNT          773 a          449 c          543 g          604 t
ORIGIN      
        1 aaaacagaca atatgaactg acaggaaaag ccatgatagt ggcatattcc cggccaattg
       61 ccatgcgaat ctatcgtaaa atgcttgaca tgcgcccagc ttggaaagat aagcttggcg
      121 ttgtcatgac ctcaggcaat gatgacccgg aagattggca caagattatt ggcaataaac
      181 agcataaagc gaagcttgca cgacaattca aagatccgga tgatccattt aagatagcta
      241 ttgtggtgga tatgtggctg acgggttttg atgtcccttc acttgcgaca atgtatgtgt
      301 ataagcccat gtcggggcat aaccttatgc aagccattgc cagggtgaac cgtgtctata
      361 aggacaagga aggcggcttg attgtagact atgttggtat tgcttctgcc ctgaagcaag
      421 ccatgaatga ctataccagg agagaccgcc agaactacgg cgaacaggac attgcaaaga
      481 ctgccctgcc caagtttgtt gagaagctgg aagtctgccg gaatcttttc catggttttg
      541 attatagtga ctttatgacc acacagagtg acctggtgcg tgccaaaacc attgccggtg
      601 gggtcaacta tctatcggct gttgaagtga aaccaacgca tgctttagat gtctggcagg
      661 ggagttcctt gcgggctgct tcaccagagc gtgaagaacc ctcaaagaaa gacttgttta
      721 tcaaagaagc catgctgctg cgccaggcat tgtcgctttg ccgttcattg ttgacggagc
      781 ctcaacgtat tgaggcggcg tacttcgaag ccgttcgtac agtgctgacc cgtcttacaa
      841 gcagtgggaa acccttgtcc ctaaaagaaa tcaacgagcg aattaacaat ctgctgaaag
      901 ccagcatcaa gagcgagggc gttatcaacc tgttttccag cgtggacagc ggattctcat
      961 tatttgatcc taagttcctg gaagaaattg gaaaaatgaa agaacggaac ttggcggttg
     1021 agctgttgcg caaactgatg gccgaacagg ttgctgtgta caggaaaaac aaccttgtca
     1081 aatctcaaaa gttctcagag cgtctaacag ccaccatgaa cgcttatctt aatggactgc
     1141 taaccaatga gcaggtaatt gcagaactga tcaaaatggc caaggaaatc aataacgcca
     1201 aggctgaagg agaagccatg gggttgacag aagaagagtt agccttctat gatgctttga
     1261 ccagtcctca agccatcaaa gatttctatg agaacgatgt actggtgtcc atgacccgtg
     1321 accttacaga aatgctgcgc aagaatcgta ctattgattg gcagaaaaag gaatcagccc
     1381 gagcgaaaat gcggcgtatg gtaaaaaagc tgctgcggaa atacaagtat ccgcctgaag
     1441 ggatggaaga tgccatcgcc acggtgattg ggcagtgtga gatgtgggtg gattgttaga
     1501 caataatgta ccatccattt cgaatactat gtagaaaaag ggaaatgatg gaatgacgtc
     1561 tatataaaat cctaaactat tatggttgtg tttaagaaat tataaaaatg gaggccatgg
     1621 gcatgccaaa tggaatgttg aattcagtaa taaaatttga tttacacatt cactcagaag
     1681 caagtaaata taaagaaagc aaaggtatag tagataactc caacaaacat aatctggcaa
     1741 cattactcaa caaactgaat gagcatgatg ttgcattgtt ttccattact gaccacaatc
     1801 gtttcgaccc agaattatac attgaaatta acaaaatatt gtctgaaaaa gataatcctt
     1861 acccgaatgt aaaggcggtg ttagctggtg ttgagtttga tgttattctt gatgatggaa
     1921 tggataagtg ccatattata gcaatatttg aaactgacaa ttgtatagaa aaattctccc
     1981 gtattgacac aggaatacgt acaaatcttt taaaagatgc tcaagaagca tattcaagaa
     2041 aagactttga aaatgtattg aaagaaattg gtctcaatac tattttgata gcatctcaaa
     2101 gaaaagacat tcaaaatcaa agtggaaaac acaattctct tagtgactcg gccataaatg
     2161 tagagcaaat aatacgtatg ggatacatta atgctctgga atttcaaaaa ccaaaagtag
     2221 aagggataat attgaatagt ttaagaaagc tttcacttcc aatcacattg ttttctggta
     2281 gtgattgtca tgattgggag cactatccat ttcacgacag tgtgaatcaa aataaagagt
     2341 tccaccattc caaagcaaag atgttgcct
//
LOCUS       DAOSRI010000259         2349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_41440_length_2349_cov_3.386661, whole genome shotgun sequence.
ACCESSION   DAOSRI010000259 DAOSRI010000000
VERSION     DAOSRI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41440_length_2349_cov_3.386661"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK717_07210"
     CDS             complement(<1..192)
                     /locus_tag="PK717_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HOR47484.1"
                     /translation="MAKKKTEKIVYGVIWFVTYLVVFMVGYIIFDIVIKGLPAISWDF
                     LTQMPSKSGSQGGILPAIIG"
     gene            complement(192..1061)
                     /gene="pstC"
                     /locus_tag="PK717_07215"
     CDS             complement(192..1061)
                     /gene="pstC"
                     /locus_tag="PK717_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOR47485.1"
                     /translation="MQKWKEKAVKLGLLGASSTSILIVLLIFFYLIKEALPFALDPGL
                     SSLSGSLWAPTSMLKESFGILPLLTGSLLVTAIATIIAVPVGVTAAIYISEIARPAER
                     EVLKPFIELLAALPSVVIGFFGLVVFVPLVKSVFGLSTGLTALTGALLLALMAIPTII
                     SISEDAINSVPGSYKQASLALGASKLETIFKITVPAALSGIVASITLGIGRVIGETMA
                     VLMVTGNAAVVTMNPLTSVRTMTATVAAEMGEVAFGSHHYRALFWVALILLGITFALN
                     ILAQKALSKYKLG"
     gene            complement(1136..2047)
                     /locus_tag="PK717_07220"
     CDS             complement(1136..2047)
                     /locus_tag="PK717_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOR47486.1"
                     /translation="MKKINIFRAAIATLGAIVLLFTAGCGGQNGNAGTNSGDGENTNP
                     PTQTVNITITGSDSMLHLCNNWAEQFMKNNKNYMITVNGGGSGVGIKALINGTTDIAS
                     ASREMKGSEKDEAKTNGNEAVENLVALDGIAIAINPENPVQDFTMTQLKDIFTGKKTN
                     WKDFGGPDQKIIILSRESSSGTYQFFQEHVLAKENYAQTALLMPATSAIIDSVAQDKW
                     AIGYVGLGYAAEAGSRIKVVPVKKDDASPAVMPSEATVKDKSYPIARALYLYTKTTST
                     SDVTKFIDFVLSSEGQNIVKEAGYITK"
     gene            complement(2159..>2349)
                     /locus_tag="PK717_07225"
     CDS             complement(2159..>2349)
                     /locus_tag="PK717_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR47487.1"
                     /translation="IPRLFERFYRVDRGRSRKQGGTGLGLAIVKHIVNAHNGHMTVES
                     IPGSGSTFAIHIPEKTNY"
BASE COUNT          538 a          513 c          585 g          713 t
ORIGIN      
        1 acctatgatt gctggcagta taccaccctg ggagcctgat tttgatggca tctgtgtgag
       61 gaagtcccac gaaatggcag gcaatccttt tatgacaatg tcaaaaatta tgtagcccac
      121 catgaacacg accagatagg tcacaaacca aataacacca tatacaatct tttcagtttt
      181 cttttttgcc attatccgag tttgtacttt gaaagtgcct tttgggccag aatattaagt
      241 gcaaaggtta ttccaagtaa tatcagggcc acccagaaca gcgccctgta gtggtgggag
      301 ccaaaggcta cttcacccat ttctgcggct accgttgcag tcatagttct aactgatgtc
      361 agtgggttca tggtcacaac ggcggcgttt cctgtaacca tcagcactgc catggtttcg
      421 ccaatgactc ttccgattcc aagcgttatg gaggccacta ttccggagag tgcggccggg
      481 acagttattt tgaatattgt ttcgagtttg cttgccccaa gggcaagaga agcttgtttg
      541 taggagccag gaacagaatt tatggcgtct tcggaaattg atatgattgt ggggatggcc
      601 atcagagcta ggagcagggc gccggtaagt gcggtcagtc cggtggatag tccgaaaacc
      661 gatttcacca gcggaacaaa aacgaccaac ccaaaaaacc ctatcacaac ggaagggagt
      721 gccgcgagaa gttcaataaa aggtttaagg acttcccttt ctgcgggtct ggcaatctcg
      781 gagatgtaga ttgcggccgt aacgcctacc ggcacagcta ttattgtggc tatggccgtt
      841 acaagaaggg agcctgtcaa aagtggcagt attccaaaag attccttgag catcgaggtt
      901 ggcgcccaaa gactgccgga aagtgaagaa agacctgggt caagcgcaaa tggaagggcc
      961 tcttttataa gatagaagaa aatgagcagg acgatcagta ttgaagtcga cgaggcccca
     1021 agcaggccaa gctttactgc cttttctttc catttttgca cgtatttgct cctgtcaaaa
     1081 aaagactttg agggtagcac caaaaggcta ccctcaaagg tttgatttct ttaccttatt
     1141 ttgttatgta gccagcttcc ttgacgatgt tctgtccttc tgaagagagg acgaaatcaa
     1201 tgaattttgt tacatcgctt gtgctcgtgg ttttggtgta gaggtaaagg gcgcgggcga
     1261 ttgggtagct cttgtctttt actgttgctt ctgatggcat tactgctggc gatgcatcat
     1321 ctttcttgac tggtaccacc ttgattctcg agccagcttc tgctgcgtag ccaagaccga
     1381 cgtatccgat tgcccacttg tcctgagcaa ctgaatcaat aattgctgat gttgctggca
     1441 tgaggagcgc tgtctgtgca taattttctt tcgcaaggac gtgttcctgg aagaattggt
     1501 aggtcccgga agatgattcc cttgagagga taattatctt ctggtctggg ccaccaaagt
     1561 ctttccaatt ggttttcttt ccggtgaaga tgtccttgag ttgtgtcata gtgaagtctt
     1621 gtacagggtt ttccgggttg attgcaattg ctatcccgtc aagggccacg aggttctcga
     1681 ctgcttcgtt tccgtttgtc tttgcttcgt ctttttcgga gcccttcatt tcgcgggagg
     1741 cagatgctat gtcggtggtt ccattgatga gcgccttgat gccaacacct gatcccccgc
     1801 cgttgacggt gatcatgtag ttcttgttgt ttttcatgaa ctgttctgcc cagttgttgc
     1861 aaagatgaag cattgaatcc gagccggtta ttgtaatatt tactgtctgg gttggagggt
     1921 ttgtgttttc gccatcgcca ctatttgttc cggcattgcc attctgacct ccgcaacctg
     1981 ctgtaaacag gaggacaata gctccaagtg tggctattgc tgctctaaag atgtttatct
     2041 ttttcatttt ttctcctttg tctgtttgcg ggtcttttat atttggcaac tgttaggcaa
     2101 ttttggtttt tctgttagga ttgtgttagc gggttaacca gttttaaaat ctgcactttt
     2161 aatagttggt tttttcggga atgtggattg cgaaagtact tcctgagcct ggaatgctct
     2221 cgactgtcat gtgaccattg tgggcattca cgatgtgttt cacaatggca aggccgagcc
     2281 ctgttcctcc ctgttttctg gagcgtcctc tatctaccct gtagaacctt tcaaatagtc
     2341 ttgggatat
//
LOCUS       DAOSRI010000260         2338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_41693_length_2338_cov_4.315813, whole genome shotgun sequence.
ACCESSION   DAOSRI010000260 DAOSRI010000000
VERSION     DAOSRI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41693_length_2338_cov_4.315813"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1746)
                     /locus_tag="PK717_07230"
     CDS             complement(265..1746)
                     /locus_tag="PK717_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47488.1"
                     /translation="MRRILLIANHDAFGRQMARWAILINKHSGWEPIIYISYDYINRH
                     MDLCRADGIRIMGPLHGEATTGHGDSTSSTSGASCWTIPKAILRRLSFLQMISRKYHY
                     SLSLLKFPLIMKSMKRNLNTIRTLLKNERIDVAMICESSPAYEAPLFIAAANMEGIPI
                     ATAPIEKSTARHYAENYLYDPSMSMKRLCNKLIGKLYPRWTISHRGYSMLRVQPELLM
                     SLELLEIAPLKPWELVGNQEDIVAVDSQSTFNHFAKEGVAPEQMAIVGRAEYDIMANI
                     KTDAKRLKSELYERLGFENNYPMILSPLVPDHYISGRPECDFQDYQKLVEFWVRSLGK
                     IKGHNAVISLHPGQTYQKKADAWDYIEQWGVKISRDDLPTLIPLCDIYVATGYSTTVQ
                     WAIACGKPVVNYDVYRYGLTIYRSFSGVLDVQEQRDFLEALERLSSDSDYYSKIAAYQ
                     QEDAEQWGKLDGRATDRLIDMFDQLISRKIGKSGQRKLMTQRQ"
     gene            complement(1775..>2338)
                     /locus_tag="PK717_07235"
     CDS             complement(1775..>2338)
                     /locus_tag="PK717_07235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOR47489.1"
                     /translation="ENTIFNPQSPYACAKTMAYYLSRYYRDTFGIFASNAILYNHESP
                     RRTPEYVTRKVTQSVARIALGQQEKLILGDLSAMIDWGYAREYMEAAWQIMQCKEPDD
                     FIIATGEAHSVKEWVQEAFSAVGLKADEHLVTDSKLFRPSNTSTLIGDISKARKVFGF
                     EPKIKFHKLVGLMVDADLETERTRLHK"
BASE COUNT          592 a          587 c          481 g          678 t
ORIGIN      
        1 cactgctgtt gtaaaaggag tgcgtagccg catcattcat ccgggggttc atgtaaacca
       61 tggaacaatt acggcatttg taatatttaa accaatcctt ttcataatgc agttttgcgt
      121 ctttctcacc acaaaccggg cagtcagcat aatgggcgaa ttcatccttc atcctcttgt
      181 tactgtaatc aaacaatagt ttatgctgct cccgtaacgc cctgttccac ctatcgagcc
      241 aatctgacga catatggtac cgtgctattg cctctgggtc atcaattttc gttgaccgct
      301 cttgccgatt ttccgagata ttaattgatc aaacatgtct attaacctgt ccgttgctct
      361 gccgtccaat ttgccccatt gctccgcgtc ctcttgctga tatgcagcga tcttcgaata
      421 ataatctgaa tcactggata atctctccaa tgcttcaaga aaatcccgtt gctcttggac
      481 atctaacaca ccggagaaag atctataaat tgtaagcccg tatcgataga cgtcgtaatt
      541 gactaccggc ttaccacagg caatcgccca ctgcacagtt gtcgagtaac ccgtcgccac
      601 atatatgtcg caaagaggaa tcaaggtcgg cagatcatcc cgggatattt tcactcccca
      661 ttgttcgatg tagtcccagg catcggcttt cttttggtaa gtctgccccg gatgtaagct
      721 gatcaccgca ttatggccct tgattttgcc cagcgacctc acccaaaact cgacaagttt
      781 ctgataatcc tgaaagtcac attccgggcg cccagatata taatgatcgg gaaccaaagg
      841 agacaatatc atgggatagt tattttcaaa acccagtcgc tcgtataatt cactcttcaa
      901 tcgctttgca tctgtcttga tattggccat gatgtcatat tctgcccgtc cgacgatcgc
      961 catttgttcc ggcgctacgc cctctttggc aaaatgattg aaggttgact gactgtccac
     1021 cgctactata tcttcctgat ttcccaccaa ctcccaaggc tttagcggtg caatttctaa
     1081 aagctccaag gacatgagca gttccggctg gacacgtagc atactgtatc ctctatggct
     1141 tatcgtccag cgtgggtaaa gtttcccaat gagcttgttg cataatcgtt tcatactcat
     1201 tgagggatcg taaagataat tttcggcata atggcgggcg gtagattttt caatcggggc
     1261 ggttgcgatc ggtattcctt ccatatttgc agccgcgata aaaagggggg cttcgtaggc
     1321 cggactgctc tcacatatca tggcgacatc tatacgttca ttcttcaaca aagtccggat
     1381 ggtattcaag tttctcttca ttgacttcat aatcagagga aatttcaaaa gcgaaaggga
     1441 ataatgatat ttcctcgaaa tcatttgtag aaaagacagc cttcgtagaa tggccttagg
     1501 gattgtccaa cacgacgccc ctgatgtgct ggaagtcgaa tctccatgac cagttgtcgc
     1561 ttccccgtgc agaggcccca tgatccttat tccatcggca cggcacaagt ccatatgtct
     1621 atttatataa tcataactta tataaataat cggctcccac cctgaatgtt tgttaatcag
     1681 gatcgcccat ctggccattt gtcggccaaa ggcatcatga ttggcaatta aaaggatcct
     1741 tctcacgcca tcgccattgc tactgaaaca ataatcattt atgcaatctc gttctttccg
     1801 tttccagatc ggcgtcaacc atcaacccta ccaatttgtg aaacttgatt tttggttcga
     1861 acccgaagac ttttctggcc ttagatatat ctccaatcaa cgtgctcgta tttgatggcc
     1921 gaaacagctt gctgtcggtc actaaatgtt catccgcctt cagaccgacg gcggaaaaag
     1981 cttcttgaac ccattctttc acagaatgag cctcaccggt ggcgatgata aaatcgtccg
     2041 gttctttgca ctgcatgatc tgccaggcag cttccatgta ttctcttgcg tatccccaat
     2101 caatcatggc acttaaatcc ccaaggataa gcttttcctg ttgaccaaga gctatccttg
     2161 caactgattg ggtaaccttg cgtgttacat attccggggt tcgtcttgga gattcatggt
     2221 tatacaagat cgcattggat gcaaagatgc caaaggtatc tcgataatag cgcgagagat
     2281 aataggccat ggttttggca caggcatagg gactctgcgg gttgaagatt gtgttctc
//
LOCUS       DAOSRI010000261         2269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_43434_length_2269_cov_3.132791, whole genome shotgun sequence.
ACCESSION   DAOSRI010000261 DAOSRI010000000
VERSION     DAOSRI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43434_length_2269_cov_3.132791"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(560..1951)
                     /locus_tag="PK717_07240"
     CDS             complement(560..1951)
                     /locus_tag="PK717_07240"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOR47490.1"
                     /translation="MVVTFIKTDSLLTEFAGLFPIVDYTHSLGLTQFIKKQFLSFERS
                     MDQFSNEQLFLGLILGSICAGPFFSDLARFFLDPGVLRVLGLKHAPDRSTILKRLDRI
                     SEMSKESLSKTIQRWCLHQFVCQKNLFGSHHKYRIVDIDSTINPAYGHQDGVTPTYVT
                     HKSGKNGLHGIHAFDFKTNFHLFGEIRPGNADSPQGTEEIVDEICEAFWSQDRKKPIV
                     FRMDSAFAESKYLNHIHEKGAKFLTKLQSNKVLVRLARQNPHWKKTRMKAANGSVIYV
                     AYLPYVNPKWSGNYRVCVYRFEEAQEEKKAPFRQKPLFEEARSRKKYQYFYLAANLDL
                     SSEKMLEMYNRRGNCENAIAQIKSQCHCCRKINRKSLQTTNLLSIFAVLGYNLSILFR
                     YGSRRCYQQNLELATFVNRYIRIPGKVVKHSRQCSIAINISKSEKKRGYQYYWESLIG
                     RTFRTLQPPGQAA"
BASE COUNT          626 a          452 c          485 g          706 t
ORIGIN      
        1 tcagcgtatt aaagattatg atatttcaat gattgttccc cagcatggtg cggtcattcc
       61 tgggaaattt gttccggatt attttgattg gctttctaac ttgcgttgtg gcgctgactt
      121 gatcaatgaa ttctattgat ttggaggatt gctgtgaaaa ccctttttcg actgtttgaa
      181 aaacaaaaag ataagaatct tgacgagcgt gattctcttt catttgtaag gcaactttct
      241 acggggcttg ttaaatcaac gattcttggg tcaacccttt cttccacaat gaagctttta
      301 aatcctaact tgtgaatgaa atttcaagaa tccaggtttt ttcccaagaa atctggattc
      361 aggtcgattt tttgtagaaa aacgaggttc gatactggga aaaagtcaga catgagtatt
      421 tgcgcaggtc aaattttgtg tcttaggttg aaatttacgt ttaaattgtg cttaaagccc
      481 ctttaaactt cattttttga caggcttcgc cctgcttgaa tgtttgctgg ggttcttgcg
      541 attggatcgg attcttcagc taagcagcct gtcctggtgg ttgtaaggtt ctaaacgttc
      601 ggccaatcag agactcccaa taatactgat agcctctttt tttctcagac ttggaaatat
      661 tgattgcaat actgcattgc cggctgtgct tgaccacttt tccgggaatg cggatatagc
      721 ggttgacaaa tgttgccaac tccagattct gctggtagca tcttctgctg ccgtacctga
      781 aaagaatgct caggttatac ccaagcactg cgaaaatcga cagtaaattg gtggtttgaa
      841 gactttttct gttgatttta cggcagcaat gacactgaga tttgatttga gcaattgcat
      901 tttcacaatt gcctctccta ttgtacatct caagcatttt ttcagaagac aaatccagat
      961 tggcagccag ataaaagtat tggtactttt tcctgctcct ggcctcttca aacagtggtt
     1021 tctggcgaaa aggagctttt ttttcttcct gcgcttcttc aaatcgatag acacaaaccc
     1081 gataattccc cgaccatttt ggattcacat aggggaggta tgcaacataa ataactgatc
     1141 cattcgcagc cttcatcctc gttttcttcc agtgaggatt ttgccttgcc agtcttacaa
     1201 gaaccttgtt tgactgaagt ttggtaagga actttgctcc cttttcatga atatgattca
     1261 gatatttcga ttcagcaaaa gcactgtcca tacgaaaaac aattggtttt ttcctgtctt
     1321 gagaccagaa tgcctcacag atctcgtcaa cgatttcttc tgttccctgc ggagaatctg
     1381 cattgcccgg tcggatttcc ccaaagagat gaaagttggt tttgaaatca aaggcatgaa
     1441 taccatgtaa tccatttttc ccacttttgt gggtcacgta ggtcggggta actccatcct
     1501 gatgaccata ggcaggattg atggtcgagt caatatccac aatccgatat ttatgatgac
     1561 tcccgaacaa attcttctga cacacaaact ggtgtaaaca ccaccgttga atggtcttgg
     1621 ataaagattc tttcgacatt tctgaaatcc gatccaatct tttgagaatg gttgaacggt
     1681 ccggggcatg cttcagcccc agtacccgca agacacctgg atcaagaaag aaacgagcaa
     1741 gatcagaaaa aaagggacct gcacagatgc ttccaaggat caatcccaaa aagagttgtt
     1801 cattggaaaa ctggtccatg gaacgctcaa aagataggaa ctgcttcttg atgaattgag
     1861 tcaatccaag tgagtgcgtg taatcaacga ttggaaacag ccctgcaaat tccgtcagaa
     1921 gtgagtcggt tttgatgaaa gtgacaacca tgtgataatc cagtataaaa cttttaataa
     1981 ttataccgta tctaatcgaa atttcatctt ttttggacag aaaacgcacc ctttgggtgt
     2041 atttttaaat tgtcgggatg tctcctttga aaggcacaaa agtccatgtc tggaaacggg
     2101 ttgattcaca agttaggtta agtggatgaa catcaatctt gtgaaaggtg gcattcttta
     2161 tgaagattcc gtgcggaaat atatctggct tggatgggaa gaagaacatg acacagggtt
     2221 ggtacaggtt aaccaatata tcttgatcga ccgggggcga ggttttttg
//
LOCUS       DAOSRI010000262         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_43514_length_2265_cov_5.652489, whole genome shotgun sequence.
ACCESSION   DAOSRI010000262 DAOSRI010000000
VERSION     DAOSRI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43514_length_2265_cov_5.652489"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PK717_07245"
     CDS             <1..1412
                     /locus_tag="PK717_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47491.1"
                     /translation="MKIRSIATLIVIFAFLLSGCGQVAPPDKGGDGGDNKQPQNTEKR
                     IYWFDDNVKTETKNGTIYSVNLDYKDMRQEFEIPKPEPQSDYVLNAGLKFYPRSHLIS
                     ISKNKKFILFSTEFVKGFDIENGETYLVDGKKVVSISVSEYSIYEPSTKEFHYIGALC
                     RYEYSAFLPWTIKEIGHKYYPAGWLDDELLLVETTKDEKIYTEDQDAAVKVSNDADEV
                     PYATAKAMSFSLKDFTLSPTDKFKPLKPWTSYSDTNTGIVGYFDPFYPFKRSLFIDYP
                     KYGSAVTTLTDFKGEKTYDLYELAKEALPKDYVLGDSSFCGAMIDKDGRRMCYYCMQG
                     YPKEILKYKPNDPVWRESYPPIPRREYIIFGLDFDTGKIEKCMDTDKINSEVQKYAKL
                     PIKTGDLVTVAPYILDGFTKESGPIFLLLYVVTHFDENGMRLDLEEEFYFLARGSKPD
                     KLEKVEIPIPMMFKQEFIR"
     gene            1500..>2265
                     /locus_tag="PK717_07250"
     CDS             1500..>2265
                     /locus_tag="PK717_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47492.1"
                     /translation="MKIRNGITNSATVLALLAVVAVLFSGCGQPTPPGKGGDGGDNKQ
                     PENAEKRIYWFDYNAKTETQSGIVYSVNLDCKDKRQEFGIPKPEPQSDYVLKTGFVFY
                     PYVNIVSISKNKKFILFSTEFAKSLNVENGETYLVDGKKVEGIEVNEYSIYEPSTKEF
                     HYIGALCKYEFNIYPPQTIKEIGHKYYPAGWLDDELLLVETTKDEKIYTEDQDAAVKV
                     SNNADEVPYATAKAVSFSLKDFTLSPTDKFKPLKPWT"
BASE COUNT          734 a          478 c          476 g          577 t
ORIGIN      
        1 agatgaaaat aagaagcatt gcaacattga ttgtaatatt tgccttttta ctctctggtt
       61 gtggccaggt agccccacca gataagggtg gggatggtgg agacaataaa caaccacaaa
      121 ataccgaaaa aagaatatac tggtttgatg ataacgtaaa aacagagacc aaaaatggga
      181 ccatatatag cgtaaatctt gactataagg acatgcgaca ggaattcgag ataccaaaac
      241 cagaaccgca atcagattat gttttaaatg ctggcctcaa attctatcct cgcagccatc
      301 tcatctccat ctccaaaaac aaaaagttca tccttttttc aacggaattt gtcaaaggtt
      361 ttgacatcga aaacggcgaa acttaccttg ttgatggcaa gaaagttgtt tcaataagcg
      421 ttagtgaata ctccatttac gagccttcaa caaaggaatt ccactatatt ggtgcccttt
      481 gcagatatga atatagtgcc tttctcccat ggaccatcaa agaaatcggc cataaatact
      541 atcctgctgg ctggctggat gatgagctcc tgcttgtaga aaccaccaag gatgaaaaaa
      601 tatacacaga ggaccaggat gccgcagtga aggtgagtaa tgatgctgac gaagtcccat
      661 acgcaaccgc aaaggcaatg tccttctccc tgaaggactt cacgctttcc ccaacagaca
      721 agttcaaacc tctgaaaccc tggacttcat attctgacac aaatacaggc atagtcggct
      781 acttcgatcc tttctaccca ttcaaaaggt ccctttttat agattaccca aagtatggtt
      841 cagctgttac caccctgaca gacttcaagg gtgaaaagac ctatgacctt tatgaattgg
      901 ccaaagaggc cctccccaag gattatgtat tgggagattc cagtttctgt ggtgcaatga
      961 ttgacaagga tggcaggaga atgtgctact actgcatgca aggataccca aaagaaatcc
     1021 tcaaatacaa acccaatgac cctgtctgga gagaaagcta tccacccatc cccagaaggg
     1081 aatatatcat atttgggctt gattttgaca caggcaagat agagaagtgc atggacactg
     1141 acaagataaa ctctgaggta cagaagtatg ccaaacttcc aattaagact ggcgatctgg
     1201 ttacagtagc tccatacatt cttgatggtt ttacaaaaga gtctgggccg atatttttac
     1261 tcctttacgt cgtaacccac tttgatgaaa atggcatgcg gttagatctg gaggaagaat
     1321 tttacttcct ggcacgtggt tccaaaccag acaagctcga gaaggtggaa attcctattc
     1381 ccatgatgtt taaacaagag tttattagat gaactcaagg tatcatcggg caagatttag
     1441 ggccaagcca gatgggcaaa gggtgtttta aactgctttt gcccttgagg aggcaacaga
     1501 tgaaaataag aaacggcatt accaatagcg ctactgtttt ggctttgctt gcagttgttg
     1561 cagtgttgtt ctccggttgt ggccagccaa ccccgccagg taaaggtggg gatggtggag
     1621 acaataaaca accagaaaat gctgaaaaaa gaatatactg gtttgattac aatgcaaaaa
     1681 cagagaccca aagtgggatt gtgtatagtg taaatcttga ctgcaaggac aagcgacagg
     1741 aattcgggat accaaaacca gagccgcaat ctgattatgt tttgaaaact ggttttgtgt
     1801 tttatccata tgtgaatatt gtttccatct ccaaaaacaa aaagttcatc cttttttcaa
     1861 cagaatttgc caaaagtttg aatgtcgaaa acggcgaaac ttaccttgtt gatggcaaaa
     1921 aagttgaagg cattgaagta aatgaatatt ccatttatga gccctcaaca aaggaattcc
     1981 actacattgg tgctttgtgc aaatatgaat ttaatattta tccaccacaa accatcaagg
     2041 aaatcggcca caaatactac cctgctggct ggctggatga tgagctcctg cttgtggaaa
     2101 ccaccaagga tgaaaagata tacacagagg accaggatgc tgcagtaaaa gtgagtaata
     2161 atgctgacga agtcccatac gccactgcaa aggcagtgtc cttctcccta aaggacttca
     2221 cgctttcccc aacagacaag ttcaaacctc tgaaaccctg gactt
//
LOCUS       DAOSRI010000263         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_43770_length_2255_cov_3.951818, whole genome shotgun sequence.
ACCESSION   DAOSRI010000263 DAOSRI010000000
VERSION     DAOSRI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43770_length_2255_cov_3.951818"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK717_07255"
     CDS             <1..838
                     /locus_tag="PK717_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOR47493.1"
                     /translation="LNFDLESLKQKKEKQIEPPLAKRAINLEQKRYEDIDFSVTNHLG
                     VPAEFKLKSPENVPPGFEDFGFATLDGLVREFATPKIIPGETFKMRFSILLSEDASPG
                     EELKDVINLEPVGYPSWKIEVPLDITVEGEAQKAPRLYFELSDEVKEATEFTAFLKIK
                     DAKGITGSKFYFVYQPSKLDILSIEDGGFFSSDGTNTAFYWSEVAKGEILVTGPVRIG
                     SLPGVDGEGVILKIRFYAKKKGEAIFDCQSSTIYDSKSKEVEHFKPEALVITIKESEP
                     SP"
     gene            835..>2255
                     /locus_tag="PK717_07260"
     CDS             835..>2255
                     /locus_tag="PK717_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47494.1"
                     /translation="MRFFAAFLALAATLYWPTFGGSPMHHSSTSVNIWPGFSKSWESP
                     LSEGSARISTPVCFENKIILKYQELKKPFEFKVVCFDVANGNVVWSFSDLVIPKPKKV
                     VLPLHPKDFEGSPTIFRNMVYVTSNDTVFCLSMNDGKPLWWKTFGNNCDVFYISPLIQ
                     GASMPAVVVPIARRSIFCLDAMKGRVIWESKNPFWATSPAFCQDNGFLCPVPMDSSGS
                     IVASIPEEMNTSLGPVITVPEKNKALFLCRDGVFRFFSYNHFFSKWEFEWKCSVDGGS
                     TYPRAACYFEGDFLLPGRENVARVNEKGKTLWNLQFGKGNIMGCLVCGKKALVLSKGT
                     SSTISVIDTRDGFLYESMKLDGQPMTVPVQTENGFVIVFAKQGLVCYKGSNPPKLDCK
                     SVEEVEVNGDGTTLLEVSNIGDSWVSFYVRSEKDWAVPENGIIRCAPGRKVMIPIKIS
                     LQGKPYRETRIVLSSETETKIVL"
BASE COUNT          647 a          434 c          535 g          639 t
ORIGIN      
        1 actaaacttt gatttggaat ccttgaagca aaagaaagag aaacagatag aaccacctct
       61 tgctaagagg gcaattaact tggagcagaa aagatatgaa gatattgatt tctcagtaac
      121 aaatcatctt ggtgttccag ctgagttcaa gcttaaatct cctgaaaatg tgccacccgg
      181 ttttgaggat tttggttttg caacacttga tgggcttgtt cgtgaatttg ccaccccgaa
      241 gataatacca ggcgagactt tcaaaatgcg tttttctata ctcttgtctg aagacgcatc
      301 tcctggtgaa gaactcaaag atgtcataaa tcttgagcct gtaggttacc catcatggaa
      361 aattgaggta ccacttgaca taactgttga gggtgaggcg caaaaagccc cgagactcta
      421 tttcgagctt tctgatgaag taaaagaagc gacagaattc acggctttct tgaaaataaa
      481 ggatgcaaaa gggataactg gatcgaaatt ttattttgtc taccaaccat caaaactgga
      541 cattttaagc atagaggatg gtggtttctt cagcagtgat ggcacaaata ctgctttcta
      601 ctggagcgaa gtagcgaagg gggagatcct tgtcactggg cctgtaagaa ttggctcact
      661 gcctggcgtt gatggtgaag gagttatcct caaaataagg ttctatgcca agaagaaagg
      721 cgaagcgata tttgattgcc agagttcgac catctatgat tcaaaatcaa aagaggtgga
      781 gcatttcaag ccagaagcac ttgtgataac gataaaggaa agtgaacctt caccttgagg
      841 ttttttgccg cttttctggc gcttgccgca acactttact ggccaacttt tggtggctcg
      901 ccgatgcacc acagttcaac cagtgttaat atctggccag ggttttccaa atcctgggaa
      961 agccctcttt cagagggttc cgcaaggatc tccacgccgg tttgttttga aaataaaata
     1021 atcctcaaat accaggagtt aaagaaacct tttgaattta aggtggtttg ttttgatgtc
     1081 gccaatggaa acgttgtctg gagtttttct gatttggtga ttccaaagcc taaaaaagtt
     1141 gtgcttccgt tgcatccaaa agatttcgaa gggtctccaa caatttttcg caatatggtt
     1201 tatgtaacca gtaatgacac cgttttttgc ctttccatga atgatggaaa gcccctctgg
     1261 tggaaaacgt ttggcaataa ttgcgatgtg ttctatatct caccgttaat acagggtgct
     1321 tccatgccgg ccgttgttgt tccaatagcc aggagatcaa ttttttgtct ggatgctatg
     1381 aaagggcgag tcatttggga gagcaaaaac ccgttttggg caacatcacc tgcgttttgc
     1441 caggacaatg gttttttgtg cccggttcca atggattcca gtggttcaat agttgcaagc
     1501 attcccgaag aaatgaacac ttcgcttgga ccggtcataa cagtgcctga aaaaaataag
     1561 gccctttttc tctgcaggga cggagttttc aggttcttct catacaatca ttttttttcc
     1621 aaatgggagt ttgaatggaa gtgttctgtt gatggcggat caacatatcc gagggcagct
     1681 tgctattttg aaggcgattt tcttttgcct ggcagagaga atgttgcgcg cgtaaatgaa
     1741 aaagggaaaa cgctctggaa cctccaattt ggcaaaggaa acatcatggg ttgtcttgta
     1801 tgcggcaaga aagcgcttgt tctttcgaaa gggacttcat ccacaatttc cgtcattgac
     1861 acacgggatg gttttttata cgaatccatg aagctcgatg gccaaccgat gacagtacct
     1921 gttcaaacag aaaatggttt tgtgatagtg tttgccaaac agggccttgt ttgctacaag
     1981 gggagtaacc ctccaaaact tgactgtaag agcgttgaag aggttgaggt gaatggcgat
     2041 ggtaccacat tgcttgaggt atcaaacatt ggtgattcct gggtttcatt ttatgtccga
     2101 tcagaaaaag actgggcagt tccagaaaat gggataatca gatgcgcacc tgggcggaaa
     2161 gtaatgatac cgataaaaat atcccttcag gggaaaccct atagggaaac aaggattgtt
     2221 ctaagctcag aaacagagac aaaaatagtt ctgtt
//
LOCUS       DAOSRI010000264         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_44219_length_2239_cov_3.271520, whole genome shotgun sequence.
ACCESSION   DAOSRI010000264 DAOSRI010000000
VERSION     DAOSRI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44219_length_2239_cov_3.271520"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>2239
                     /locus_tag="PK717_07265"
     CDS             24..>2239
                     /locus_tag="PK717_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47495.1"
                     /translation="MITVNGTPLNKPPQVVKTLNQVTITSPVAINNLSEVAVVFSDKI
                     GMKNPAKIADYTLKVKTDSEPETIESEPFKVATVPVVEVNPNMEYNTAEYYAVGILPA
                     DSPIKSGQEFTVNFPKGTSIPKTIQPSSVIVNNQPYKGVVNAGLETISLVAQSDIVGA
                     LKVRFLVNAGIKNTAPGTYELTFTTGGKPFTFEKFEIVKSKPIIANIDITEEQGCEQS
                     GYKFTYIPSFAGDLNPGDKLTVEFPENTRLPQAAPGDKVIIGGKPSQAVAIDGLKLVI
                     TVDTEVKAANGGALVDITRDAGVINTNYPGGYSLKVWTDKDESVSSPQFTVTMPKVTS
                     QLAFKDPAQPDGLEFEGCLWYKTPPVLSITSCNPYAKIFMWYDNKTDSIVQYTGEKRL
                     NQGSLRSTIWYYAQVGDVKEEPKPMRLCLDTIPPSFSVIQPDMEKTITNKKTYTIKGE
                     RQPTEMLTDGDNEKYQVVDGVYVNGKQLLTPEIFEVANRESITMNFETTVDLKEGENV
                     VEIKGIDQAGNERVVKKIIVLDTKAPGIVVVKPDPKELQKPGDNIAVQVESETDASVF
                     IGGQIAQKIQELPDGKTAIFEGNWDVVKGENKVEITSTDVAGNTSTTYFTFKGGSKST
                     VIELWLKKTDFIVNGEKMPNLATAPVSSSPPLPKALAGSTFMPIADVAKALGVKVDWD
                     QKTKMVTLTQTLDNGTQKVIQLWINKKQAKIDGKDVWIDAKKVLYPTILSGKTLLPL"
BASE COUNT          734 a          556 c          482 g          467 t
ORIGIN      
        1 tccctggatc tatcaaacca aaaatgatca ctgtaaatgg gaccccgctc aacaagccgc
       61 cacaagtcgt caaaacactg aaccaggtaa caatcacttc ccctgttgca atcaataacc
      121 tcagcgaagt tgcagtagtt ttctctgata aaatcggaat gaagaacccg gcaaagatcg
      181 ctgactatac cctcaaagta aaaactgact cagagccaga aacaatcgaa tcagaaccgt
      241 tcaaagtggc aactgttcca gtagtcgaag taaatccaaa catggaatac aacactgctg
      301 aatattacgc agtcggcatt cttccggcag attcaccaat caagtccggc caagaattca
      361 cggtcaactt cccaaagggg accagcattc caaaaacaat ccagccatca tcagtcatag
      421 taaacaatca gccatacaag ggcgttgtca atgccggcct ggagacaatt tctcttgttg
      481 cccaatctga tattgtcggc gcattgaagg taaggttcct tgtaaatgca ggcatcaaga
      541 acaccgcacc aggaacatac gagctcacct tcacgaccgg cggaaaacca ttcacattcg
      601 agaaatttga gattgtcaaa tccaaaccaa taattgccaa tatcgatata actgaggaac
      661 agggttgtga acaatctgga tacaagttca cctacatacc atcatttgca ggcgatctta
      721 accctggcga caaactgacg gtcgagttcc cggagaacac cagactcccg caggcagcac
      781 caggagacaa ggtaatcatc ggcggcaaac catcccaggc agttgcaatt gatggtctta
      841 agctcgtcat aacagtagac acagaggtca aggcagcaaa cggcggagcg cttgtagaca
      901 ttacacggga tgctggcgtc ataaacacca actatccagg cggatattca ctcaaggtct
      961 ggacagacaa ggacgagtct gtttcttcac cacagttcac agtcacaatg ccaaaggtga
     1021 cctcacagct tgcattcaag gacccagcac agccagacgg cctcgaattc gagggctgcc
     1081 tctggtacaa gacaccaccg gtgctttcaa tcacaagctg caacccgtac gcaaaaattt
     1141 tcatgtggta tgacaacaag accgattcaa ttgtccagta cactggcgag aagaggctca
     1201 atcagggctc actgcgttca acaatctggt actatgccca ggttggagac gtgaaggaag
     1261 agccaaaacc aatgaggctt tgtctcgata caattccgcc atcattctcg gttatacagc
     1321 cagatatgga aaaaaccatc accaataaaa agacttacac catcaaaggt gaacgccaac
     1381 caactgaaat gctcactgat ggcgataacg agaaatacca ggttgttgat ggtgtctatg
     1441 tcaacggcaa acagctcctc accccggaaa tattcgaagt agcaaacagg gaaagcatca
     1501 caatgaactt tgagacaaca gtagacctta aggaagggga gaatgttgtc gaaataaagg
     1561 gtattgacca ggcaggaaat gaaagggtag tcaaaaaaat tattgtcctc gacaccaaag
     1621 caccaggaat tgtggtcgtc aaaccagatc caaaagaact ccagaaacca ggtgacaaca
     1681 tcgccgtcca ggtggaatcc gagaccgatg cttcagtgtt cattggcggc cagattgccc
     1741 agaagataca agagcttcct gatggaaaaa cagccatttt tgaaggtaac tgggacgtcg
     1801 tcaagggtga aaacaaggtt gaaataacct ccacagacgt ggccggtaac acttcaacaa
     1861 cctacttcac attcaagggt ggatcaaagt ctacagtcat cgaactctgg cttaaaaaga
     1921 ctgactttat agtcaatggg gagaagatgc caaatcttgc tacagcacca gtgtcttctt
     1981 caccaccgct tccaaaagcg cttgcaggta gtacgttcat gcctatcgct gatgttgcga
     2041 aagctcttgg agtaaaagtt gattgggatc aaaaaaccaa gatggttaca ctcacccaaa
     2101 ccctcgataa tggcacccaa aaggtcatcc agctctggat aaacaagaaa caggcaaaga
     2161 ttgatggcaa ggatgtctgg attgatgcaa aaaaggttct ttatccaaca atcctttctg
     2221 gcaaaaccct tcttccact
//
LOCUS       DAOSRI010000265         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_44358_length_2233_cov_4.006887, whole genome shotgun sequence.
ACCESSION   DAOSRI010000265 DAOSRI010000000
VERSION     DAOSRI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44358_length_2233_cov_4.006887"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..>2233)
                     /locus_tag="PK717_07270"
     CDS             complement(40..>2233)
                     /locus_tag="PK717_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47496.1"
                     /translation="FFGTIDSLLCSFSTSGGEKSWERELPKCITGIASDKSCIYFGGL
                     DKKIYCANKSDGSTKWEYAIDNIPAASPVVGGSMVYASSMSGTFYVLDAETGDLVWSY
                     KTGGPVLSAVSIYDKRVFVGSMDGNMYCFSSDTVTQKTSLEKIVVTSESPNVKIKESV
                     LLSAVGYDSEGKKIGINPKWSVDPPEMGTIASSGTFTAGTKAGTCTVKACVGNICGEA
                     KIEVIEPQILSKIEVKPERKEMFIGESAVFEAVAYDQNGKQIPNIAFVWSTNNASVGS
                     IDSNGLFLANGPGSCNVVANLGDVRGVATVVVEDIEKIELEPDKINIKKGQTYQIKVY
                     VVTLSGRRAELNKELKWSLDPEGQGNITKTGLFTAPNEPALKGSIVCEYLGKTARAEY
                     TVEDDRRANITLSTNLLDFGELAAGQTKTLKLVLKNAGNKDAQIKITADYDFLVIGQK
                     EFLLEAGFQKEMAIIADSTKLKPGNAYSGSILVAWETGESSVKFVLKTAEKPKNCPKL
                     SPSEFYFGDIKRGKSMSLPFSLDFPHSGTYKGTIKASASWIEVNPPTFAINGNRFGGT
                     ISVKASAIPKGTDFEEEITIQVEDCPEIKAKLKVRTENSIFVKLVLGEKKALLNDDEL
                     ELDVPPQPVGGRTLIPVRFISECFGCKVDWLPSEGRITITRNDFKIVIWKDKKEALVN
                     GQPFVLDVPASIIAGRTLVPLRFISEAFGAKVEWNQSTKGITILWEPF"
BASE COUNT          542 a          587 c          456 g          648 t
ORIGIN      
        1 cctgaggaac accattaacc ggcctggttc accaactctc taaaatggct cccagagaat
       61 tgttatgccc tttgtactct ggttccattc caccttggcc ccaaaagcct cggagataaa
      121 cctgagtggt acaagtgttc tgccggcaat aatggatgct ggaacatcaa gaacaaatgg
      181 ttggccgttc accagcgctt ctttcttgtc cttccagatg actattttaa aatcgttcct
      241 cgttatggtt atcctgccct cggagggcaa ccagtcaacc ttgcagccaa aacactccga
      301 aataaacctg acagggatga gcgttcggcc gccaaccggc tggggcggga catccagctc
      361 aagctcgtca tcgtttagca gggctttttt ctcgccaagc acgagtttga cgaatatgct
      421 attttctgtt cgcactttca gctttgcctt tatctcgggg caatcttcaa cctggattgt
      481 gatttcttcc tcaaaatcgg tgccttttgg aattgcagac gcttttaccg agatggtgcc
      541 gccaaacctg tttccattaa tcgcaaatgt cggcgggttc acttcaatcc agctggcgct
      601 ggcctttatc gtgcctttgt aggttccgct atgcggaaaa tccagggaga aaggcaaaga
      661 cattgatttt ccacgcttga tgtcaccaaa atagaactca cttggtgaca acttggggca
      721 atttttcggc ttttctgcag ttttcagcac aaatttgacg ctcgattcac ctgtttccca
      781 tgccaccaaa atggaaccac tataggcgtt tccaggtttc agttttgtac tatcagcaat
      841 tatggccatc tccttttgaa aaccggcttc cagcaaaaac tctttttggc caatgaccag
      901 aaaatcatag tctgccgtaa tctttatttg cgcatctttg ttgccggcat ttttcaaaac
      961 cagcttcagt gtctttgtct ggcccgcagc caattcgcca aaatcaagca aatttgttga
     1021 cagggtaata tttgcccttc tgtcgtcctc gacggtgtac tcagctcttg cggttttgcc
     1081 aagatattca cagacgatag aaccttttaa tgcgggctcg tttggggcgg taaacaggcc
     1141 tgtttttgtg atgttcccct ggccctccgg atcaagagac cattttaatt ccttgtttaa
     1201 ttcggccctt ctgccagaaa gggtcacaac atagactttt atctggtaag tctgcccttt
     1261 tttgatgttg atcttgtcag gctcaagctc gatcttttca atgtcctcca ctaccactgt
     1321 agccacaccg cgaacatcac ccaggttcgc aaccacattg cagctgccag gcccgtttgc
     1381 caaaaacaga ccattcgaat cgattgaacc gacggatgca ttattagtgc tccagacaaa
     1441 tgctatgttg gggatttgct tgccattctg gtcgtatgcc acagcctcaa acactgcgct
     1501 ctcgccaata aacatctctt tgcgctccgg tttgacctca atctttgaaa gaatctgcgg
     1561 ttcaattacc tcaattttgg cctcaccgca tatattgcca acacaggctt ttaccgtgca
     1621 agttccagct tttgtccctg ctgtgaatgt tccactggac gctatggtgc ccatttctgg
     1681 cggatccaca gaccattttg gatttatgcc aatttttttg ccctccgaat catagcctac
     1741 ggcagaaagc aaaaccgatt cttttatctt gacgtttggt gattcagaag taacaacaat
     1801 cttttccagc gatgtttttt gggtgacggt gtcactggaa aaacaataca tgtttccatc
     1861 cattgacccc acaaagaccc ttttgtcata aattgagaca gctgacagga ccgggccacc
     1921 agttttatat gaccagacca gatcgccggt ttctgcatcc agaacataaa aagtacctga
     1981 cattgaactg gcatacacca tcgatccccc aacaacagga gaagcagcgg gaatattgtc
     2041 tattgcgtat tcccacttag tgcttccgtc agatttgttg gcacaataga tcttcttgtc
     2101 cagccctcca aaataaatgc aagacttgtc agatgctatg ccagttatgc attttggcaa
     2161 ttccctttcc cagctctttt ccccaccaga tgtgctaaac gagcacagca ggctatctat
     2221 ggtgccaaaa aac
//
LOCUS       DAOSRI010000266         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_44836_length_2217_cov_3.983349, whole genome shotgun sequence.
ACCESSION   DAOSRI010000266 DAOSRI010000000
VERSION     DAOSRI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44836_length_2217_cov_3.983349"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1844
                     /locus_tag="PK717_07275"
     CDS             177..1844
                     /locus_tag="PK717_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47497.1"
                     /translation="MFCGISRVETGNQDITDLVASIKITEACGEPSSCTIETVLLQAD
                     QIPQYLPFCQVSVFGEGGEVVFGGRCDGVPKKEETPLGTRLVFEARDWSAECKHRMVN
                     EHYMEWRASDIYKSLVAKHLPGHSVDLVRDCDDTVSVKFGGQSVEQCLKTLCDFTGWV
                     FRVSPQKIHYFGPAFLEHLQVTIRDPHCDMSKGQTTPFEPDYSTLANRVWVEGGEGDA
                     ENFTKQYILSSELPDTPDPNICTLYPFAFPIWYKCSSLSVYAVFDSGDSLLERGSLVL
                     LDCDTDGLSVKNSVVKTNEPLDTKPALSSLILHDEADTNLPGTFSSDHKITVNFDALY
                     CPDATAEALGGWVYLGRSVKEAKGLLGFMAKYKRKLKISYVSEADEESVRKYGVQIDL
                     PKVTKPAIKDWAILYKYADYLLSSHKDPPRKGKATVYRYKGGQMVYPVPIRAGVAADF
                     EFERFGACEGARISRVTHEITPMQWRMVCESSLDPNLYASLLADVIKRLAQLEQEASE
                     GDIINAASGLSSGVGVMDFMPSIKSFLPKNDYGFGESLFGACEWEGQ"
     gene            1841..>2217
                     /locus_tag="PK717_07280"
     CDS             1841..>2217
                     /locus_tag="PK717_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47498.1"
                     /translation="MKEWLENIKQAIGEVSDEAGAYFALKGRLHGRVTIGDQIRENTV
                     TDAGIRAIARALCSPVRQSGRTLSPVFRIEWFAASTSATPPTPKDTTLGGSVSLRSVE
                     QVDALGKKLVVTNFVGQPEFNFT"
BASE COUNT          559 a          524 c          630 g          504 t
ORIGIN      
        1 gttatgggac aggcatgcaa actggcgccg gaaagattga aatcaatcag acattcaatc
       61 ttgaccttgg cggctacgaa ggggacatat cctcgcttgc cagggagatt gcccagcaaa
      121 cagtcattga agcccagaaa gcccttctgg agcttgtcaa tttcaggggg taggcgatgt
      181 tctgcggcat atcaagggtt gaaacgggca atcaggacat aacggacctt gttgcctcga
      241 taaaaataac cgaggcttgc ggcgaaccat catcttgcac aattgaaact gtcctgcttc
      301 aggcagacca aatcccccag tacctgcctt tttgccaggt cagtgttttt ggcgagggcg
      361 gggaggtggt ttttggaggg aggtgtgacg gggtgccgaa aaaagaggag acaccccttg
      421 gcacgaggct ggtttttgag gcaagggact ggtccgccga gtgcaaacat cgtatggtca
      481 acgagcacta catggaatgg agggcatctg acatctacaa gtctcttgtg gccaaacacc
      541 ttcccggaca ctcggtagac ttggtcaggg actgtgatga caccgtttcg gtgaaatttg
      601 gcgggcagag cgtggagcag tgcctcaaaa cactctgcga ttttacagga tgggtgtttc
      661 gcgtttcacc gcaaaagatt cattatttcg gcccagcatt tctggagcac ctccaggtca
      721 cgataaggga cccgcactgc gacatgtcaa aagggcagac aacacctttc gagcctgatt
      781 attcgacact tgcaaacagg gtctgggtcg agggtgggga gggcgatgcg gagaatttta
      841 caaagcagta catcctttcc tccgagttgc cagacacgcc tgacccgaac atctgcacgc
      901 tttatccgtt tgcctttccg atatggtaca aatgttccag tctttcagtc tatgccgtct
      961 ttgattctgg tgacagcttg cttgaaaggg gcagccttgt tctgcttgat tgtgatacgg
     1021 acggtctttc cgtgaagaat tctgttgtaa agaccaacga gccactggac acaaaaccgg
     1081 ccctgtcgtc ccttattttg catgatgagg ccgacaccaa tcttcccggg acattctcct
     1141 cggaccacaa gattacagtc aatttcgatg ccctatattg tccagatgcg acagcagaag
     1201 ccctgggtgg atgggtatac ctgggcagga gcgtcaagga agccaagggg ctcctaggct
     1261 tcatggccaa atacaaaaga aagctcaaaa taagctatgt ttccgaggca gacgaggagt
     1321 ctgtcagaaa gtatggcgtc cagatagacc tgccaaaagt caccaagcct gcaataaaag
     1381 actgggcgat actttacaaa tatgccgact accttctttc atcccacaaa gacccgccga
     1441 ggaagggcaa ggccaccgtg tatcggtaca agggcgggca aatggtctat ccagttccta
     1501 taagagccgg cgttgctgcc gattttgagt ttgagaggtt tggggcatgt gaaggggcca
     1561 ggatttcgag ggtcacacac gaaatcacgc cgatgcaatg gaggatggtt tgcgagtcca
     1621 gcctcgaccc caatctctat gccagcctgc ttgccgatgt catcaagagg cttgcccagc
     1681 ttgagcagga ggcatctgaa ggggacatca tcaatgcggc atcggggctt tcttctgggg
     1741 ttggggtgat ggattttatg ccgtcaataa agagttttct gcccaaaaac gactacggtt
     1801 ttggtgagtc gctttttggg gcatgcgaat gggagggaca atgaaagaat ggctggaaaa
     1861 cattaagcag gccattggag aggtctccga tgaggcaggt gcatattttg cactgaaggg
     1921 gaggctccac gggagagtga caattggcga ccagataagg gaaaatactg taacagacgc
     1981 aggaataagg gccatcgcga gggcactttg ctctccagtc aggcaatcgg gcaggacgct
     2041 ctcaccggtt ttcaggatcg agtggtttgc cgcctcgact tctgcaacac cgccaacacc
     2101 aaaagacaca acgcttggcg ggagtgtttc gttgaggagc gtggagcagg tggacgcatt
     2161 gggaaaaaag ctcgtagtaa ccaactttgt tggccagccg gagttcaatt tcacatg
//
LOCUS       DAOSRI010000267         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_44945_length_2213_cov_3.142725, whole genome shotgun sequence.
ACCESSION   DAOSRI010000267 DAOSRI010000000
VERSION     DAOSRI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44945_length_2213_cov_3.142725"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..735)
                     /gene="plsY"
                     /locus_tag="PK717_07285"
     CDS             complement(64..735)
                     /gene="plsY"
                     /locus_tag="PK717_07285"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOR47499.1"
                     /translation="MDTFSQAKELLIFALLIIVPYIIGSIPFGLLIGLAFGIDIRGKG
                     SGNIGATNVQRTLGWFPGLASWILDTAKGFFPVYLTSYMLENNFFGQEFAGASTGWPI
                     ALVGLAVIAGHCWSVFLKFRGGKGVSTTLGVVMALDWKIGIACFILWVIIVALTRYVS
                     VASMITAILMPFGFIFLGADPIFWVLCFAIAIIIVVKHQDNIKRLREGTENKFGSGRN
                     SAHKS"
     gene            complement(737..1732)
                     /gene="pilM"
                     /locus_tag="PK717_07290"
     CDS             complement(737..1732)
                     /gene="pilM"
                     /locus_tag="PK717_07290"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOR47500.1"
                     /translation="MKIGPIGIHLGDQRIKIAQVASDKDRIQIIEMVSLPTPEGSIIQ
                     GFVKAPRVAADAIMRAISESKFNGRKAVIAHPTEILKVHTMTVQDSAELDKAISTKLS
                     QMSFEQPEELIFDYKIAARENGRVKAIVAITNKKHVQKIQELATQSKLTLGGTDLEML
                     STYRVVAGTYRTPDTPTVIAMLSDQNVKLALFCERVLSYVKTTDLQSSQAKSDPKQLS
                     SEIGKFLGEYRKTNLLTGEPVIFLAGLPEPSFEMEKAVWEELDSRTTSVRWSEAFSLS
                     PTYNNFGELTTRFGAFCCAIGLSLSDMQLPQSFKSPIIPDVRQPGYTPDLLKFEG"
     gene            complement(1729..>2213)
                     /locus_tag="PK717_07295"
     CDS             complement(1729..>2213)
                     /locus_tag="PK717_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOR47501.1"
                     /translation="VTFIPNILEIVIKLSEEYPIMSTRMIDPGMIKKGVKRKIIGFSI
                     LCLGSKFVFGLGRYIKFWLKKLEEAGLVYPDALVSWELFGESDCEASLLKSLATLGDG
                     ITEIFGHPGLVDEELLGISTYTDIRERELAAFTSARVKDAITQSDTELINAFCLGGHL
                     "
BASE COUNT          581 a          505 c          464 g          663 t
ORIGIN      
        1 agttcaccca tcgaggccat tcctgaagct gcctttatca tggattcttt cgggttttgt
       61 ggatcaactc ttatgagcac tgttcctccc agaaccaaac ttgttttctg tcccttccct
      121 caatcttttt atgttatctt ggtgtttgac gacaataata attgcgattg caaaacagag
      181 gacccaaaaa ataggatctg cccccagaaa aataaaacca aatggcatca aaatggcagt
      241 gatcattgat gcaacactta catatcttgt caaagcgaca ataattaccc acaaaataaa
      301 gcaggcgatt cctattttcc agtcgagtgc cattacaacg cctaatgttg ttgaaacccc
      361 tttgccaccc ctgaatttca ggaaaaccga ccagcagtgg ccggcaatca cagctaaccc
      421 aacgagggcg attggccacc ctgtgctggc tccagcgaat tcttgtccaa aaaaattgtt
      481 ctcaagcata tagctggtca aataaacggg aaaaaagcct tttgcggtat cgagtatcca
      541 tgatgcaaga cccggaaacc acccaagggt cctttgtaca tttgttgcac ctatatttcc
      601 ggaacctttg cctcttatat caattccaaa agccagacct atcaggagcc cgaatggaat
      661 tgagccaata atgtatggaa caataattaa taatgcaaaa ataagcaatt cttttgcctg
      721 ggaaaatgta tccatttcag ccctcgaatt ttagaagatc tggagtgtat ccgggttgcc
      781 tcacatcagg aatgatgggg cttttaaaac tttgtggaag ttgcatgtcg gagagtgaaa
      841 gtccgattgc acagcagaag gcaccaaaac gggtggtcag ttcgccaaaa ttgttgtaag
      901 ttggtgaaag cgaaaaagcc tccgaccatc tcacagatgt tgttcggctg tcaagttcct
      961 cccaaacagc tttttccatt tcaaagcttg gctccggcag tccagcaagg aaaatgaccg
     1021 gttcccctgt cagaaggttt gtttttctgt attcgccaag gaattttcca atctcggacg
     1081 atagctgctt tgggtcgctt ttggcctgtg atgactgaag atcggttgtt tttacatacg
     1141 acagcaccct ttcgcagaaa agagccagtt tgacattctg atcggagagc attgcgataa
     1201 cagttggtgt atcaggtgtc ctgtatgttc ctgcaaccac tctgtaggtt gaaagcattt
     1261 ccaggtcggt tccaccaagg gtcagttttg actgtgtggc caactcctgg atcttttgca
     1321 catgtttttt gtttgttatt gcgacaattg cctttaccct tccattctcc cttgcagcaa
     1381 ttttgtagtc gaatatgagt tcttctggtt gctcaaaaga catctgggag agttttgtcg
     1441 agatggcttt gtcgagctct gcactgtcct ggaccgtcat tgtgtggacc tttaggattt
     1501 cggtgggatg ggctattaca gcttttcttc cattgaattt tgattcggat atggccctca
     1561 tgattgcatc tgccgctacc cttggtgcct tcacgaaacc ttgaataatc gaaccttccg
     1621 gggttggcag ggacaccatc tcgattatct gtattctgtc tttgtcagat gcaacctgag
     1681 ctatctttat tctttggtca ccaagatgga ttcctattgg cccaattttc atagatggcc
     1741 tcccaagcag aaagcgttta tgagttcagt atctgattgt gtgatggcat ctttcaccct
     1801 tgctgatgtg aaagctgcga gctccctttc ccttatgtcg gtatatgttg atatgccaag
     1861 gagttcttca tcaaccagac cagggtgccc gaaaatttcc gtaataccat cgccaagtgt
     1921 tgcaaggctt tttaaaagtg atgcttcgca atcggactcg ccaaaaagct cccatgaaac
     1981 caaagcatca ggataaacaa gccctgcttc ttcaagcttt tttagccaga acttgatgta
     2041 tcttccaaga ccaaatacaa attttgatcc aaggcaaagt attgaaaaac caataatttt
     2101 tctttttaca ccctttttga tcatgcctgg gtcaatcatt ctcgttgaca ttattgggta
     2161 ttcttctgat agcttgataa caatctcgag gatgttaggt atgaaagtta cat
//
LOCUS       DAOSRI010000268         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_45650_length_2188_cov_4.943272, whole genome shotgun sequence.
ACCESSION   DAOSRI010000268 DAOSRI010000000
VERSION     DAOSRI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45650_length_2188_cov_4.943272"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..359)
                     /locus_tag="PK717_07300"
     CDS             complement(84..359)
                     /locus_tag="PK717_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47502.1"
                     /translation="MNWYLSCAYLYKISFSHSDDTSIVKYLMRPLDIIYSNQWSGVTT
                     AIKNFSGMITAIISFLMVKQFRVRGVIPCYQYFIHQLMRTTDSDIMS"
     gene            375..719
                     /locus_tag="PK717_07305"
     CDS             375..719
                     /locus_tag="PK717_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47503.1"
                     /translation="MLASKIKEIKNGRVIVKDSKTKDMLEDKYKTFLDKLRKSGKTNM
                     FSAAPFLQQMFDLSPLEARKILQQWMQEFESDPRTEDEAVDPTKPKNVAAAKTKAGAM
                     GAAGASRSGSMS"
     gene            782..1438
                     /locus_tag="PK717_07310"
     CDS             782..1438
                     /locus_tag="PK717_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOR47504.1"
                     /translation="MHETPEGNYCQCGCGRIAKHKFVHGHNNRGMEFSEEHKKKIGQA
                     NSISQKGRVPWNKGLSKETDIRVCHSEEAKEKIKKNNAKYWEGKRRDLETNKKVSNTV
                     LKLWTNPEYREKHEGENHPQWSNGASFLPYTQDWTEDLKDSIRKRDGYRCKICSLSQE
                     EQGRKLDVHHIDYDKTNCDPYNLVSLCDSCHAKTGFGNRETWRAYFHRRKIVRRERRA
                     "
     gene            1438..>2188
                     /locus_tag="PK717_07315"
     CDS             1438..>2188
                     /locus_tag="PK717_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HOR47505.1"
                     /translation="MPDRFPFYVTEQLSEHMYETPEGFLVCMDVPVARMGEYTYKSSE
                     VPIEGGKDGLVKIIRDEDEVFSEEAIKSFNSKPVTINHPNDFVTPDNWKDLAHGTIQN
                     ARRGQGEQSDLLIADLVITTEDAIKLIKAGLREVSLGYDAQYDQLEPGLGKQKDIIGN
                     HLALVVKGRAGNRCAIMDKACECCAGCSCGKNNSPKKEGETEMKKTAMKDVLRKIFPR
                     LNLDHVKDEDLEIGEGTSAGSDVEVAQQAAAE"
BASE COUNT          787 a          331 c          536 g          534 t
ORIGIN      
        1 cccgtacaga gattgcgaag atgtccacag ggctggtcaa ggcaagagcg gtatcgtcag
       61 atatcaactg gtatgtatgg cggtcaactc atgatatccg agtccgtagt tcgcataagc
      121 tgatggataa agtattggta gcatgggatg acgccccgaa ccctgaattg cttgaccatc
      181 agaaaagaga ttatggcagt tatcatcccg gagaaatttt taattgccgt tgttacgcca
      241 gaccattgat tagaatagat gatgtccaat ggccgcataa ggtatttcac aatggaagta
      301 tcatcagaat gagaaaagct gattttataa agataagcgc aggacaaata ccagttcaat
      361 aaaggagaga taagatgctt gcaagcaaaa taaaggaaat caaaaatggt cgggtgattg
      421 taaaagattc caaaaccaag gacatgctag aagacaaata caaaaccttt ctggataaat
      481 tgagaaagtc cggtaaaacg aatatgttta gtgctgctcc tttcttgcaa cagatgtttg
      541 atctttctcc attggaagca agaaagatac ttcaacaatg gatgcaggaa tttgaaagtg
      601 atccgaggac tgaagatgaa gcggttgacc ccaccaaacc gaagaatgtt gccgcagcaa
      661 aaaccaaggc aggagcgatg ggcgcagcag gggcatccag aagcggaagc atgagctaat
      721 taaaaaggat gaacaaatgg aactgaaacg attttatacc acagagcaat tgtcggagca
      781 catgcatgaa actccggaag gtaattattg ccaatgtggt tgtggacgaa tagcaaaaca
      841 taaatttgtt catggacata ataatcgagg gatggaattt tctgaagagc ataagaaaaa
      901 gattggtcag gcaaatagca tcagtcaaaa gggaagagta ccgtggaata aaggtttgag
      961 taaagaaaca gatattcgag tttgtcattc agaggaagct aaagaaaaga taaaaaagaa
     1021 caatgcaaaa tactgggagg ggaagagaag agatttagaa acaaataaaa aagtatctaa
     1081 tactgttttg aagttgtgga caaatcctga atatagggaa aaacatgaag gagaaaatca
     1141 tcctcaatgg tctaatggag cttctttcct cccttacact caggattgga cagaggattt
     1201 aaaagattcc attcgcaaaa gagatggtta tcgatgcaag atttgttctt tgtctcaaga
     1261 agaacaagga cgcaaattgg acgttcatca tattgattat gataaaacaa attgtgaccc
     1321 ttacaatctt gtttctttgt gtgatagttg tcacgccaaa actggatttg ggaatagaga
     1381 aacgtggaga gcttattttc ataggagaaa aattgttcgg agagaaagga gggcttgatg
     1441 ccagacagat tcccatttta tgtaacagaa caattatcgg agcatatgta tgaaactcct
     1501 gaaggttttt tggtatgtat ggatgtccct gtggccagaa tgggagaata tacatataag
     1561 tccagcgaag ttcctattga aggaggtaaa gacggacttg taaagataat tagagatgaa
     1621 gatgaagtgt tttctgaaga ggccattaaa tcttttaatt caaaaccagt aactattaat
     1681 catccaaatg attttgttac accggacaat tggaaggatt tggctcatgg tacaattcaa
     1741 aatgcaagaa gaggccaagg agaacaatcg gatttattga ttgctgattt ggttattaca
     1801 acagaagatg caattaaatt aataaaagcg ggattaagag aggtaagtct gggctatgat
     1861 gcccaatatg accagcttga acctggatta ggaaaacaaa aagacatcat aggcaatcat
     1921 cttgccttgg tcgtaaaagg gagggcagga aacaggtgcg caattatgga taaagcgtgt
     1981 gaatgttgtg ctggttgttc gtgcggaaaa aataatagtc caaagaagga gggtgaaacc
     2041 gaaatgaaaa aaacagcaat gaaagatgta ttgaggaaaa tcttccccag attgaatctt
     2101 gatcatgtga aagatgaaga tttggaaatt ggtgaaggta catcggcggg aagtgatgtg
     2161 gaagttgccc agcaggcagc agcagaag
//
LOCUS       DAOSRI010000269         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_45768_length_2184_cov_3.829967, whole genome shotgun sequence.
ACCESSION   DAOSRI010000269 DAOSRI010000000
VERSION     DAOSRI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45768_length_2184_cov_3.829967"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK717_07320"
     CDS             <1..349
                     /locus_tag="PK717_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOR47506.1"
                     /translation="KFFNHLVERGAKVILYPLSVLKATWENPLQVWKEAYDHELFISS
                     KINSLVKVAKEIEDFTSMPLLHWFLEEQIEEEKQTQEVVALLERAGSSGMSLIYADQG
                     LGHRKPGEGSALA"
     gene            455..1288
                     /locus_tag="PK717_07325"
     CDS             455..1288
                     /locus_tag="PK717_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOR47507.1"
                     /translation="MENSKIQNFKNERDRLNKTLSEYAGKTMKDFMGLDYRCYQAGAL
                     TEKTKELMGLVASLVLRCDDCINYHLMQCLEKNVSDEELVDTLTIGLIVGGSITIPHI
                     RRAFDVWDESKTASFEANRKESFSKVIGAIKGMLAKTQNPRETLEAVCLLLKNNVPHY
                     DWVGYYITDPNKPDELVLGPYVGEPTEHTRISFGQGICGQAADKKANFIIQDVSKETN
                     YLSCSFRVKSEIVVPIIDGDKVIGELDIDSHKVSPFTEEDEAFLEEMVGLTKIYVKQI
                     L"
     gene            1360..>2184
                     /locus_tag="PK717_07330"
     CDS             1360..>2184
                     /locus_tag="PK717_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOR47508.1"
                     /translation="MPGIYKAVKVTDKVWWVGVIDRSLKEFHGYRTPRGTTYNAYLVV
                     ADKITLIDTVKKPHFPEMMERISSVVDPSKIDYIISNHAEMDHSGCLTEAMEAIKPEK
                     VFASTVGASVLRDELHGIGEITPVKDGETLNLGSMNATFIESRMLHWPDSMMTYLDSE
                     KLLFSQDAFGMHLGTYKKFDDEIPWDMLEWEAKTYYANIILPYSDFVLKFFDKLGGLN
                     LDIKTIAPDHGPIWRSRIPQILELYKKMAAQKFCERAIVVYDTMWHSTEMMAKAICE"
BASE COUNT          677 a          497 c          501 g          509 t
ORIGIN      
        1 gaaatttttc aaccaccttg tggaacgtgg cgcaaaagtt attctctatc ctctctcggt
       61 cctgaaggcc acatgggaaa acccgcttca ggtatggaaa gaagcctatg atcatgaact
      121 cttcatttct tccaaaatta acagcctcgt gaaagtggcc aaagaaattg aagatttcac
      181 atcgatgcca ctgctccact ggttccttga ggaacagata gaggaagaaa agcagaccca
      241 ggaagtagtt gccctgcttg agagggcagg ttcttccggc atgtcactga tttatgcaga
      301 ccagggactc ggccatcgaa agccgggtga gggctcagca ctggcatagg aaccatgctt
      361 aaccaaaaag gcgggggtat taccccgcct ttttttgtgc tgcatatttt ttgaaaatct
      421 tttgcagttt acccaaaaaa tgtaaaatgg acacatggag aactctaaga ttcaaaactt
      481 caagaacgaa cgcgacagac tgaacaaaac cctttcagag tatgccggca aaaccatgaa
      541 agattttatg gggctcgatt acaggtgcta ccaggctggc gctctcacgg aaaaaaccaa
      601 ggagctgatg ggacttgtcg cctcactggt gctccgctgt gatgactgta tcaattacca
      661 cctcatgcaa tgcctggaaa aaaatgtcag cgacgaggaa ctggttgaca cgctcacaat
      721 cggtctcata gttggaggat caataacaat tccccacatt agaagggcgt tcgatgtctg
      781 ggatgagtca aaaacagcat cttttgaggc caatcgcaag gagagttttt caaaggtaat
      841 tggcgcgata aaaggcatgc tcgcaaaaac acaaaatcca agagaaacgc tcgaggcagt
      901 ttgcctgttg ctcaaaaata atgttccaca ttacgactgg gtcggttact acatcacaga
      961 cccaaacaaa ccagacgagc ttgtccttgg gccgtatgtc ggggaaccaa ccgagcacac
     1021 aagaatatct ttcgggcagg gcatttgtgg gcaagccgcg gacaagaaag ccaatttcat
     1081 cattcaggat gtttcaaaag aaacaaacta cctctcgtgc tcgttcaggg tcaaatcgga
     1141 aattgttgtc ccgatcatcg atggagacaa agtgattggc gagctggaca tagattcaca
     1201 caaggtctcc ccctttacag aagaagacga ggcgttcctt gaagagatgg ttggactcac
     1261 gaaaatatat gtaaaacaaa ttttgtaaaa tgataaaatg taattcaata caatgcttta
     1321 tttttatatc atcttgtgta aatttctaac ggaggaacaa tgccaggtat atacaaagca
     1381 gtaaaagtga ccgataaagt gtggtgggtt ggcgtcatcg accgttcgct caaggaattc
     1441 catggataca ggacgccaag gggaaccacc tataacgcgt accttgtagt ggccgacaag
     1501 ataacactta tcgacactgt caaaaaaccg cattttccag agatgatgga aaggatttcc
     1561 tcggtggtcg atccttcaaa aattgactat atcatctcga accatgccga aatggaccat
     1621 tcaggttgcc tgactgaggc catggaagca ataaaacctg aaaaagtctt tgcttccact
     1681 gtaggagctt ctgtgctcag ggatgagctg catggcatag gcgagataac gcctgtaaaa
     1741 gacggtgaaa ccctcaatct tggtagtatg aatgcgacat tcatcgagtc aaggatgctc
     1801 cactggccag acagcatgat gacctacctt gacagcgaaa aactgctttt ctcacaggac
     1861 gcgtttggaa tgcaccttgg cacctacaaa aaatttgacg acgaaatccc ctgggacatg
     1921 cttgagtggg aagcaaaaac atactacgca aacatcatcc tcccctactc cgattttgtc
     1981 ctcaagttct tcgacaaact cggcggatta aacctggaca tcaaaaccat cgcaccagac
     2041 catggcccga tctggaggtc caggattcca caaatcctcg aattgtacaa aaaaatggct
     2101 gcacagaaat tctgcgagag agcaatcgtt gtctacgaca cgatgtggca ctcaactgaa
     2161 atgatggcaa aagcaatttg tgaa
//
LOCUS       DAOSRI010000270         2181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_45854_length_2181_cov_4.053622, whole genome shotgun sequence.
ACCESSION   DAOSRI010000270 DAOSRI010000000
VERSION     DAOSRI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45854_length_2181_cov_4.053622"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK717_07335"
     CDS             <1..637
                     /locus_tag="PK717_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47509.1"
                     /translation="ARYRVNILKNAHSMTTEAQNALLKLLEDGKPNCTNVLLSESLDE
                     ILPTIQSRSIILRLPPLPVGPSAALLVEKGLAEEIAKRESESFLGDISLSAWMAENTE
                     KTEQLLQVMTSFGEGTVLPLLAVLEDDENLEYILHLVREVLHRAVLLKHGIHIDGAFL
                     EKAVPKIASMPQERLQTIERALAETERLWKTQSKKTTLAQAMFLRAWTSSA"
     gene            619..1314
                     /gene="ricT"
                     /locus_tag="PK717_07340"
                     /pseudo
     CDS             619..1314
                     /gene="ricT"
                     /locus_tag="PK717_07340"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(1538..>2181)
                     /locus_tag="PK717_07345"
     CDS             complement(1538..>2181)
                     /locus_tag="PK717_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOR47510.1"
                     /translation="FFRDPPRTSLGDSSVIVSPCDGMLMYVKKIENGQVECEKLGTKI
                     KVEEISKSDDKPQKGLLFGIYMSPFDVHFNYAPISGKITKVVHTQAKLNLPMVDLWEY
                     IQFCWLRRAVNLLGAKYQLVNERNTIFIEGPIKMAMIEIADKVVNKIDCFVSENDYIN
                     QGQKVSFIKRGSQVDLVLWGDDYDVLVHPGLHVTGAETVLARVGHQAGHGVGA"
BASE COUNT          643 a          490 c          522 g          526 t
ORIGIN      
        1 cgccaggtac agggtgaaca tcctcaagaa cgcacactcc atgacgactg aggcccagaa
       61 cgccctgctc aagcttcttg aggacggaaa gccaaactgc accaacgttc tcctttcgga
      121 gagtcttgac gagatactgc caacgataca gtcaagatca atcattttga ggttgccgcc
      181 actccctgtc ggcccttctg ccgccctgct tgttgaaaaa ggtcttgcag aagaaattgc
      241 aaaaagggag tctgaaagtt ttctgggcga catctctctt agcgcgtgga tggcggaaaa
      301 cacagagaaa actgaacagc tgcttcaggt catgacaagt tttggcgaag gtacagttct
      361 ccctttgctt gctgttcttg aagatgatga aaatctcgaa tatattttgc acttggtgcg
      421 agaagttctc catagggcgg tccttctgaa acacggaatt catattgatg gtgcattcct
      481 tgagaaggct gttccaaaaa ttgccagcat gcctcaagag aggctgcaaa caattgagag
      541 ggccctcgca gaaacggaaa ggttatggaa gacacaatcg aaaaaaacga cgttggcaca
      601 ggcaatgttc ctgagggcat ggacgtcgtc ggcataaaat tcaggggaag caacaaggta
      661 tatcactttc ttacggacga gatcggatta aggcccggtg atgccgtggt tgccctcact
      721 gaaaaaggta ttgatattgg tcatgtgacc tacacaaccc aaaaagtgga gaacccggca
      781 gggctcaaaa aactgctccg cagggcaaca gcgcttgacc ttataaggtt ccatgaaaat
      841 atggccctgg agaatgttgc cagggttgag gcccagaaac ttgctgattc aatgaatctc
      901 cagatgcagt ttgttgttgc ctgctacaac ctcgacaggt caaaggtgac cctgtatttc
      961 atggctgaaa agagaattga cttcagggaa tttgtcaggg aactctccaa aaaactccat
     1021 attaaagttg aactgtggca gatcggacaa agggacgaaa cgagactgtt tggtgggatt
     1081 ggcccatgca acaaggtttt ttgttgcgca actttcctca actcaaaaga gtcagtcacc
     1141 attaaagatg caaaatgcca gaggctcgat attaacccac agaagataac aggaatgtgt
     1201 ggaaaactga tgtgctgcct gaagtatgag gttgacacat acaaagaagc gttacaggat
     1261 tttcctgaag acggggaaac ggtgctttat tcaggccagg agtgcaaggt ccagagcgta
     1321 aatccgtttg ccaggactgt aacagttaca ggcccagaag tgtctatcgt aacggtcaag
     1381 atagaggaaa tatcaagaaa ctaccatggc gagtggatcc ctgcaatcgg ctcgtatgag
     1441 aagatgtcag agcaaaaagc tgaaagagca agaatcagct ttgaaaatgc agaactggca
     1501 aaaaccagga agaaaaaaaa gaaagataaa ccgagtgtca ggcccccaca ccatggccag
     1561 cctggtgacc aaccctcgca aggacagttt cagcaccagt cacatgaagt cctggatgaa
     1621 caagaacatc ataatcatcc ccccacagaa caagatcaac ctgagagccc cttttgataa
     1681 agctgacttt ttgcccctgg ttgatgtaat cgttctctga tacgaaacag tctatcttgt
     1741 tgactacctt gtcggcaatt tcaatcatgg ccatctttat agggccctca ataaatatgg
     1801 tgtttctttc attcacaagc tgatattttg ccccaagaag gtttactgcc ctgcggagcc
     1861 aacaaaactg tatgtactcc cagaggtcaa ccattggcag attcagcttt gcctgtgtgt
     1921 ggacaacctt tgttattttc cctgatattg gcgcgtagtt gaaatggaca tcaaagggag
     1981 acatgtatat tccaaaaaga agccctttct ggggtttgtc atcagatttg gaaatttcct
     2041 caacctttat ttttgtaccg agtttttcgc actcaacttg cccgttttcg atctttttta
     2101 catacatgag catgccgtcg cacggcgaga caataacaga tgagtctcca agagaagttc
     2161 ttggtgggtc cctgaagaac c
//
LOCUS       DAOSRI010000271         2173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46087_length_2173_cov_3.753541, whole genome shotgun sequence.
ACCESSION   DAOSRI010000271 DAOSRI010000000
VERSION     DAOSRI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46087_length_2173_cov_3.753541"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK717_07350"
     CDS             <1..653
                     /locus_tag="PK717_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOR47511.1"
                     /translation="PRQMKPISIVGAPLKEKPLIFIKCVPEKPRPEEPFLLLIKTSFI
                     PSLSSIKLDIDTDPAFYQVSSIKALDVYGNSKENVLFENAVDNEKGKVGIKYLSTNGS
                     SVDFKGTIFAIECISHQEGISSVSIKECTATSSFGQIAPIPLKAVFEIKKGYSFYDLN
                     MDGVVDELDGDILMYRLGTSEGEENYTESMDFDGDGQITVIDFIILAKHYGQTMYP"
     gene            715..>2173
                     /locus_tag="PK717_07355"
     CDS             715..>2173
                     /locus_tag="PK717_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR47512.1"
                     /translation="MRRYLVWALIFVLSAGLFPASRVLSASDIYLVTINGESACRMES
                     MGFSPIHSMLVSKTLLASAKDAIRKNCHSASFRFDFDYLLNGMSVELDKRDAEVVKSL
                     SFVKSITRINSLAPAAVRTVAETVGASPLYEVKDQNGNFVNGKDILVGVIDSGIDYRH
                     QDLGNGMYGPGAKVVGGSNFVEPKAPPLDDEVMGHGTSVAGIIAADNTKAPGIAQGAK
                     LMSYKVFSNKNPDVMEDVVIAAIQAAVKDGCKVINVSLARPGGSSSELSASALAAEQA
                     TRAGISVVGSAGDFGVYCDGIEGGTVGGPSLALDSICVGSSDTRPACRVKIEGSNRSI
                     FGMMSVPPLSFAGPQSIELVDGGYGSEEEFSKTNVLGKYVLIKRGPEIGEAIPFFQKL
                     LNAKKRGAVGAIIWNHSLGEVFQMFVGFNSITGEPIRNEEIVPSCFIGNSDGLFLQKL
                     VSGQEVKTSITEEIADTVARMTAIGPTSDLTFKPDV"
BASE COUNT          616 a          410 c          511 g          636 t
ORIGIN      
        1 cgccccgtca aatgaagcca atttcgattg ttggcgcacc tctaaaggaa aaacctctga
       61 tatttattaa gtgtgttcca gagaaaccac gccctgaaga acctttcctt cttcttataa
      121 aaacgagttt tatcccaagc ttgtcttcca taaaactgga cattgatact gatcccgcat
      181 tttatcaggt gagctctata aaagcactgg atgtttatgg caactccaaa gaaaatgttt
      241 tgtttgaaaa cgccgttgac aatgagaagg gcaaggtggg tataaagtat ctttccacca
      301 atggttcttc cgtggatttt aagggcacca tttttgctat tgagtgcatc tcacatcagg
      361 aaggcatctc gagtgtttcc ataaaagaat gtaccgcaac ctcttctttt ggccaaattg
      421 ctccaatacc tctcaaagca gtttttgaga taaaaaaggg ctacagtttt tatgatctaa
      481 atatggatgg tgttgttgat gagcttgatg gtgacatact catgtaccgt cttggaacat
      541 ctgaagggga agaaaactat actgaatcga tggattttga tggagatgga caaataaccg
      601 taattgactt tataatattg gcaaaacact acgggcaaac aatgtaccca tagactagca
      661 taagcatttc attttggtat aattcttacg cgatactaga tttaaggagc ttcaatgaga
      721 agatacctgg tttgggcttt gatttttgtt ctctcggcag gtctttttcc ggccagccgg
      781 gtcttgtcgg cttctgatat ctatctggtt accattaatg gtgaatcggc ttgcagaatg
      841 gaaagtatgg gtttttcgcc gatccattcg atgctcgtgt caaaaacttt gcttgccagt
      901 gcgaaggatg ctatcagaaa aaattgccac tcggcttcat tcaggtttga ttttgattac
      961 cttttaaatg gaatgtctgt tgagcttgac aagcgggatg ctgaagtggt aaaaagcttg
     1021 tcatttgtta agtcgattac aagaattaat agtctagcac cagcagcagt caggactgtt
     1081 gctgaaacag ttggggcctc gccgctttat gaagttaagg atcaaaatgg caattttgtt
     1141 aatggcaaag acattcttgt cggtgtgatc gattctggaa tagattatag acaccaggac
     1201 cttggaaatg gaatgtatgg acctggtgca aaggtggtgg gtggatccaa ttttgtggaa
     1261 ccaaaggcac ctccgcttga tgatgaagta atgggtcatg gaacctcagt agcaggcatt
     1321 atcgctgcag acaacacaaa agcgccgggt attgctcaag gtgcaaaatt gatgtcttat
     1381 aaggttttct caaacaagaa tcccgatgta atggaagatg ttgttatcgc ggcgattcag
     1441 gcagctgtaa aagatggatg caaagtaatc aatgtctctc ttgcaagacc aggtggcagt
     1501 tcttctgaac tttcagcctc tgctcttgca gcagaacagg ctacaagggc tggtatttca
     1561 gttgttggct cagccggtga ttttggagtt tattgtgatg gcattgaagg tggaacggtt
     1621 ggtgggccat ccctagccct tgattcgata tgcgttggtt cttcagacac caggccagct
     1681 tgcagggtaa aaattgaagg ctcaaacaga agcatttttg gaatgatgtc tgtgccaccc
     1741 ctttcatttg ctggaccaca atccatagag cttgttgatg gtggatatgg atccgaagag
     1801 gaattttcaa aaactaatgt tcttggcaag tatgttctta taaaaagagg gccagagatt
     1861 ggcgaagcaa tcccattctt ccagaaactg ctcaatgcaa aaaagagggg tgctgttggt
     1921 gcaattatct ggaaccatag tcttggagaa gttttccaga tgtttgttgg tttcaattca
     1981 ataactggag aaccaataag aaatgaagaa attgtcccat catgttttat agggaattct
     2041 gatggactct tcctccaaaa gcttgtctca ggccaggaag tcaagacctc tatcacagaa
     2101 gaaattgccg atacagtggc aagaatgacg gctattggtc caacatcgga cctaacattc
     2161 aaaccggatg ttt
//
LOCUS       DAOSRI010000272         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46237_length_2168_cov_3.536678, whole genome shotgun sequence.
ACCESSION   DAOSRI010000272 DAOSRI010000000
VERSION     DAOSRI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46237_length_2168_cov_3.536678"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK717_07360"
     CDS             complement(<1..808)
                     /locus_tag="PK717_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR47513.1"
                     /translation="MRASYGTLQLLGLKPGGFPFSPRTAFFLIGEECSGNCAFCAQSA
                     GQSDKLSRITWPEIDPNEAIEKLAFIQLDRICIQAVKSHPAFREACEFLPKIKKANSA
                     PISASMHIENLAQTELLFDLGCDSVSVALDCSTEELSRVYKGRDLSSSFNLLQELATK
                     WPGKVATHLIMGLGETEEELVKLARKIIKAGASISLFAFTPIEGTLLENNTPPEPKRY
                     RMVQVALALIRKNQDINLHYQNGKLVFEVLPRQLLEPEDFQNPGCSGCNRP"
     gene            complement(805..>2168)
                     /locus_tag="PK717_07365"
     CDS             complement(805..>2168)
                     /locus_tag="PK717_07365"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47514.1"
                     /translation="DQGTIFTIKFKALISGKAIIGITKLQGRKINFDQVELKGESGNF
                     LIKEPATKPVLQVDPTELDFGSIKYDESKTLKVHVSNMGKEGLSGNVEIHNIWIISDL
                     EKFAKEEFDLSITVAPKEEHNLIVNSNYTGRLSIFTNGGSKDVVCKFYLQDKTPINNL
                     PPDLTVDEPKDGTIFSTNKIKLKGRTNPTAKIYLNGTPQPVASDGSFEYDLMLLEGEN
                     KIEVRAISEAGLEATKIIKVKLDTIKPVVSIKDPGEIVHSQTLCIKGTTEPGTTIKAG
                     GKPVSPDQSGAFSVCFDLKKGINELSLIVTDQAGNITEWKKTVNYVAQEIIKMTMWVG
                     SPRAQVNGQTIYLEAPPTVKNGRTFVPVRFVSENMKANVIWNQKTRSVTVEGKSHKCI
                     VSIGSDTAFLDGDVVRLEAAPFIQGGYTMVPLRFIAQDTLGATVNYDSKDKRIDLELV
                     IEL"
BASE COUNT          487 a          482 c          483 g          716 t
ORIGIN      
        1 atggtctgtt acatccactg cagccggggt tctggaaatc ttcaggctcg agaagttgcc
       61 ttggcaggac ctcaaatacc agttttccgt tttgatagtg caaatttata tcctggtttt
      121 ttctgattag cgccaatgcg acttgtacca tcctgtatcg ttttggttct ggtggggtgt
      181 tgttttcaag cagggttccc tcaatcgggg tgaaagcaaa caaggagatg ctggctcctg
      241 cttttatgat ttttcttgcg agtttcacca gttcttcttc agtttcccca agtcccatta
      301 tcaaatgtgt tgccaccttg ccaggccatt ttgttgctag ctcctggagc aagttaaacg
      361 aactggataa atctctgccc ttgtaaaccc ttgaaagctc ctctgttgag caatcaagcg
      421 caactgacac tgagtcgcat cccaaatcaa aaagcagttc tgtctgtgcc agattttcaa
      481 tgtgcattga tgcagatatt ggcgcactgt ttgccttttt tattttgggt agaaattcac
      541 aggcttccct aaatgcgggg tgtgatttga ctgcctggat gcaaatcctg tccaattgta
      601 tgaaggcaag tttttcgatg gcctcattag ggtcaatctc tggccaggtt atccttgaga
      661 gcttgtcgct ctggcctgcc gattgcgcac aaaaggcgca atttccggaa cattcctcgc
      721 caataaggaa aaatgccgtt cttggactaa atggaaagcc gccgggcttt agtcccagca
      781 gctggagtgt gccgtatgaa gccctcacaa ttcaatcact agctccagat ctatgcgctt
      841 atcttttgaa tcataattga ctgtggcacc aagcgtgtct tgggctatga accttagcgg
      901 gaccatggta tagccaccct gaatgaacgg ggccgcttcc agccttacga catcaccatc
      961 aaggaaagct gtgtcagagc ctatggaaac gatacactta tggcttttgc cctcaacggt
     1021 gactgatctt gttttctggt tccagatgac attggctttc atgttttcgg aaacgaatct
     1081 gacagggaca aatgttctgc cattttttac tgttggcggt gcctccagat aaattgtctg
     1141 cccgttcact tgtgcccttg ggctgccaac ccacatggtc attttgatga tttcctgggc
     1201 cacataattg acagttttct tccattccgt aatgttgcca gcctggtcgg taactatcaa
     1261 agaaagttcg tttatgccct tcttgaggtc aaagcaaaca gagaaagcac cggattgatc
     1321 gggtgatacc ggtttcccac ctgcttttat tgttgtgcct ggttctgttg tgccttttat
     1381 acagagcgtc tggctgtgga caatttctcc tggatccttt attgaaacca ccggcttgat
     1441 tgtatcgagc tttaccttga ttatttttgt ggcttcaagt ccagcttcgc taatagccct
     1501 cacttcaatt ttgttttcac cttcaagtag cataaggtcg tactcgaatg aaccgtcgga
     1561 tgctacgggc tgtggtgtgc cattgagata aatctttgcc gtaggatttg ttctgccctt
     1621 caattttatt ttgtttgtgg aaaatatcgt gccatctttt ggctcatcaa ctgtgagatc
     1681 tggaggcaga ttgttgattg gtgttttatc ctgcaagtaa aatttgcata cgacatcttt
     1741 tgagccacca tttgtgaaga ttgagagcct tcctgtgtag tttgaattta caataagatt
     1801 gtgctcctct tttggagcga cggttattga aagatcaaac tcttcttttg cgaatttttc
     1861 aagatcggat attatccaga tattgtgtat ttcaacattt ccagagagac cttctttgcc
     1921 catattggaa acatggacct taagcgtttt gctctcgtcg tattttattg atccaaaatc
     1981 cagttcagtt ggatcaacct gtaagactgg tttggtggct ggctctttaa tcaggaaatt
     2041 tcctgattca ccctttagtt ccacttggtc aaagttgatt tttctcccct ggagttttgt
     2101 aatgccaatg attgctttgc ccgatatgag ggccttgaat ttgattgtaa agattgttcc
     2161 ctggtctt
//
LOCUS       DAOSRI010000273         2164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46352_length_2164_cov_3.378378, whole genome shotgun sequence.
ACCESSION   DAOSRI010000273 DAOSRI010000000
VERSION     DAOSRI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46352_length_2164_cov_3.378378"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK717_07370"
     CDS             <1..533
                     /locus_tag="PK717_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOR47515.1"
                     /translation="ALVTHVLGPEIVHRISIVSRGMALGYTLPLPVEDKYLSSKDELE
                     RNIAGILGGTAAERVVFDEISTGASNDIRKATDIARKMVTEYGMSSLGPIAFGHPAEM
                     VFLGRDFSASPDYSDETGRMIDQEIRRIISDGLETAIKTVQENRGTMDKLVEVLIEKE
                     TLEAEELQKLLFDAKK"
     gene            530..1324
                     /locus_tag="PK717_07375"
     CDS             530..1324
                     /locus_tag="PK717_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOR47516.1"
                     /translation="MTWRYYNTVPMRGARNMAYDEVMLKTAPPSSVTLRTYTWDPACL
                     SLGCFQKYSEVNLEKIKENGFDVVRRSTGGRAVLHDKELTYSIVITPPHEILNMNVLD
                     SYYLLCEGIVSGLNFLGVNAALKKGDDRELSTPSCFAAPTFNDIESGGRKLVGSAQMR
                     AKEGLLQHGSILLDVDIKKLFDVVSKDENQSSRLAEAAKAKITSVNEQLGFRIGLEQL
                     KENILNGFSDSFSVGFEEVTLDGDFFAKVDDLEKNKYNSAVWTENR"
     gene            1331..>2164
                     /locus_tag="PK717_07380"
     CDS             1331..>2164
                     /locus_tag="PK717_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47517.1"
                     /translation="MKKILTFMIVAIIALSPLVLVEAGNNEATDWFCFQKDHQRTGTA
                     FFSEKISTPLIQAWDGIIDTNIYIAQNIDPVKGTIIIGKDIAVAATEKGKIGAFSTLD
                     GKPVWRRDIGLPLTTDMALSDEVLLVPLKGGKVLGLDLYTGGTFWEYQAKGEVKSPPL
                     PYNGYFYFPTTEGKLVQLTSASGLVSNEFNMNVNVQLPMAVQPVGYSVSSYAQMIMNS
                     SDGNMVAYNPSTKDFDWQMKLDSPTAVGPLPIGESIIVCSKTGQVNAYALSDRKKYWS
                     YD"
BASE COUNT          611 a          449 c          530 g          574 t
ORIGIN      
        1 atgcccttgt gactcatgtt cttggcccgg agattgtcca tagaatatcc attgtttcga
       61 ggggaatggc ccttggatac accctccctt tgccagtaga agacaaatac ctgagctcga
      121 aagacgagct tgagaggaac attgctggga tacttggcgg aactgccgcc gagagggtgg
      181 tgttcgacga aatatcaaca ggtgcttcca atgacataag gaaggccact gacattgcaa
      241 gaaaaatggt caccgagtat ggaatgtctt ctcttgggcc aatagctttt ggacatccag
      301 ccgaaatggt tttccttggc agggatttct cggcttctcc agattattct gatgaaaccg
      361 gcaggatgat agaccaggag ataagaagga tcatatccga tggtcttgaa acagccataa
      421 agactgttca ggaaaacagg ggaaccatgg acaagctcgt cgaagtgctt attgaaaagg
      481 aaacattgga ggcagaagag ttgcaaaagc tgctctttga tgccaaaaaa tgacttggag
      541 atattataac accgtcccaa tgaggggcgc caggaacatg gcttatgacg aggtgatgct
      601 gaaaactgca ccgccttcat cagtcacgct caggacatac acctgggatc cggcatgcct
      661 ctctttaggg tgtttccaaa aatattccga agttaatctt gagaagataa aagagaatgg
      721 ttttgatgtc gtgaggaggt cgactggcgg aagggcagtt cttcacgata aagagctgac
      781 ctattcaatc gtcatcacgc cgccccatga gattttgaac atgaatgttc ttgattcgta
      841 ttacctcttg tgcgaaggga tagtctccgg gctaaatttc cttggtgtga atgctgccct
      901 taagaagggc gatgacaggg agctttctac gccgtcttgt tttgccgctc caacattcaa
      961 tgacatcgag tctggcggaa ggaaacttgt tgggtctgca caaatgagag caaaagaggg
     1021 ccttctccaa catggttcaa tactgcttga cgtggacata aaaaagttgt ttgatgttgt
     1081 cagtaaagat gaaaaccagt catcaaggct tgctgaggct gcaaaagcaa aaatcacaag
     1141 tgtcaacgag caactgggtt ttaggattgg tctggagcaa ttgaaagaaa acattttaaa
     1201 tggtttttcc gattcattta gtgtcgggtt tgaagaggtc acccttgatg gtgatttctt
     1261 tgccaaagtc gacgatcttg aaaaaaataa atacaattcg gccgtatgga ccgagaatag
     1321 gtagggtggg atgaaaaaaa ttcttacttt tatgatcgtt gcaattatcg cattgtcccc
     1381 attggtgttg gttgaagccg gcaataatga agcgacagac tggttctgtt ttcagaaaga
     1441 tcaccagagg actggtactg ctttcttctc cgaaaagatt tccaccccac ttattcaggc
     1501 atgggatggt atcattgata ccaatatcta tatcgcccag aacatagatc ctgtcaaagg
     1561 tacaatcatc attggcaaag acatagctgt tgcggcaact gaaaaaggga agataggcgc
     1621 tttcagtacg ctcgatggta aacctgtttg gaggagagat attggtttac ctctcacaac
     1681 agacatggca ctctcggatg aagtgcttct tgttccgtta aagggtggaa aagtccttgg
     1741 gcttgatctc tatactggtg gcaccttctg ggaataccag gcaaaaggtg aggtaaaatc
     1801 tcctcctctg ccatacaatg ggtatttcta ttttccaacc accgaaggca agctcgtaca
     1861 gctcacctcg gcctcggggc ttgtttcaaa tgaatttaac atgaatgtga acgttcaatt
     1921 gccaatggca gttcagcctg ttggctacag tgtgtcttct tatgcccaga tgatcatgaa
     1981 ttcatcagat ggcaacatgg ttgcatacaa tccatccacc aaggatttcg actggcaaat
     2041 gaagcttgat tcaccaactg ccgttggccc attgccgata ggcgaatcaa tcattgtctg
     2101 ctcaaaaact ggccaggtca atgcttatgc tctctctgac agaaagaaat attggtctta
     2161 tgat
//
LOCUS       DAOSRI010000274         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46540_length_2157_cov_4.023787, whole genome shotgun sequence.
ACCESSION   DAOSRI010000274 DAOSRI010000000
VERSION     DAOSRI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46540_length_2157_cov_4.023787"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1131)
                     /gene="selD"
                     /locus_tag="PK717_07385"
                     /pseudo
     CDS             complement(172..1131)
                     /gene="selD"
                     /locus_tag="PK717_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            complement(1205..>2157)
                     /locus_tag="PK717_07390"
     CDS             complement(1205..>2157)
                     /locus_tag="PK717_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47518.1"
                     /translation="IPSPSIYEALRPVLMNLQSEDGQTDKYFFTSKNSLFEADFQSMK
                     CINLGQIPTACQIRRLGPMSDFAVGVFLPDGKCGSILSSRYAQKLDYGWNAGFAPKSH
                     ADVYAFWSGKELIGLNITTGEVDWRHEFDRSIICALYDPGENLWYVDFGDWNKLQPIE
                     AQTGNLLEERQKDQRFTMASSNDGLYLGHTTTENFGQINFFALPDGQVVAKSSSGELW
                     RKSLEGLISSNCFDPSKDIGTIYAKGVDEFILLDLLTGETTISVGSKLISFPTADSAF
                     TDKGFWIKGQLVAPKCKSVSYVDGSFIIGWTDGVSCLIIP"
BASE COUNT          628 a          501 c          429 g          599 t
ORIGIN      
        1 taaacaggat tgaggcaaga gtttttccag aaaacactag atcacagagt gtcctgagaa
       61 atattggttt ccaatatgaa ggtacattgc gtcaacaaca ggtcattaaa ggcaaatatc
      121 aggatttgat gatgttctcg ttgctgaaaa gcgaatttag atagcccttt tttatttgga
      181 aaggattact ctcggtgaac cactggcaac acgacctatc acctgaccaa aacatcctga
      241 acccccaagg gcactaagac aatctgaaac cttatcttca tcaacaaaac caatcaatcc
      301 gcccgatgtc tgcgcatcat tggcaatatt gaggaaatat gattccacac cctgtccaat
      361 aacaagatta tcacccatca tgtccatgtt ttgtgcaaga acagggcatg taaaatactg
      421 ggaggcttca aggacaccct ccatgattgg tatttttgat atttcaatgt gcatttcaca
      481 tccagatgat tgcgccactt ccagaagtga aacagcaaga ccatatcctg taacatctgt
      541 cgagcaattg atgccaattt ctgaaaaaat cttcgatgca caggtatttg tttttaccat
      601 ccagctcaca gctttttcaa attcttggct gtatttggcc ttgccagcat aaagaatgcc
      661 agttcccaaa ggttttgtaa gtattattgc ctggcctggt tttgccctgt ccgaaagcct
      721 gattttgtct tttgcgacag tcccgaaaac cgaaaggcca tattttacat cattgttctt
      781 ggctgtatgc ccgcctgcga tgagaacgtc attttcaatg gccgacaggg cacaacccct
      841 tattatttca ccaagcttgt ctatttcgac atcccttggg aacaaggcaa cattaagagc
      901 aagcactggt gttccaccca tcgaaagaat gtcgcttatg caattggcaa aagctatcct
      961 gccaaatgtt acaggatcat cgacgattgg agagagtaca tcaacggtaa acacgccata
     1021 aagaccgtct ccaatctcat aaacggccgc atctgccttt gacataccag ctgtgaggag
     1081 cttctcggat ttgaattccg ggagggattc aagcaatttt tgtagggccg acggccccca
     1141 tttgcttgct cagccagcac aatcggtaaa ttttgtgagc tgaaaaacct ctttttcagt
     1201 catatcaagg gattataaga caagaaacac catctgtcca cccaattatg aagctcccgt
     1261 caacataaga cacacttttg cattttgggg caacaagctg gccctttatc caaaaacctt
     1321 tatccgtaaa agcagaatct gctgttggga aagaaataag ttttgagcct actgagattg
     1381 tggtttctcc ggttagcaaa tccagaagta tgaattcatc gacccctttt gcataaatcg
     1441 tgccaatatc cttggatgga tcaaaacaat ttgaagatat caaaccctcc agggattttc
     1501 tccacagttc acccgaggat gatttggcaa caacctggcc atctggcaaa gcaaaaaaat
     1561 ttatctggcc aaaattttct gttgtcgtat gcccaaggta gagaccgtca ttcgagcttg
     1621 ccattgtaaa acgctgatct ttctgtcttt cttccaggag gtttccagtt tgtgcttcaa
     1681 taggctggag tttgttccag tcgccaaaat ccacatacca gagattctcg ccaggatcat
     1741 acagggcgca aatgattgac ctgtcaaact catgcctcca gtcaacctca ccagttgtaa
     1801 tatttaggcc aattaattct ttaccagacc aaaaggcgta gacatcagca tgcgatttcg
     1861 gtgcaaaacc agcgttccat ccataatcca gtttttgggc atagcgcgaa gataagatgc
     1921 ttccacattt gccatcaggc agaaacacgc caactgcaaa atccgacatt ggacctagcc
     1981 ttctgatctg gcatgctgtc gggatctgcc caagattgat acatttcatt gattggaaat
     2041 cagcttcaaa aagggaattt ttggatgtga aaaaatattt gtctgtctgg ccatcttcgg
     2101 attgaagatt catcaataca ggtcttaatg cttcataaat tgatggcgag gggattt
//
LOCUS       DAOSRI010000275         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46673_length_2153_cov_3.775500, whole genome shotgun sequence.
ACCESSION   DAOSRI010000275 DAOSRI010000000
VERSION     DAOSRI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46673_length_2153_cov_3.775500"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK717_07395"
     CDS             complement(<1..444)
                     /locus_tag="PK717_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR47519.1"
                     /translation="MTAFSYRSLLNKFPAIILAILFLAGCGGGAVTIETNPKGGTIIF
                     EGKEYTSPAQLELEEGVHKIEAKVTGYLPAQEEILYKKGQGDRFVISLSKEPVESDSI
                     KPVLVDKAVRIRTSPKSTNSFLYVYDPLTGKIGKELDLETTPIQGD"
     gene            complement(410..1453)
                     /locus_tag="PK717_07400"
     CDS             complement(410..1453)
                     /locus_tag="PK717_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47520.1"
                     /translation="MMNISRRLHNTPALVFISVCVMLSSCGKSAPALDKTFENSHLSI
                     AYPSLYQVAEKDNVVFIKSENVEFSITYAPDASSKTGDTSSINQLVSSLTQTLTGKII
                     KNEPVELEKNKIWWIETQNPESVIALFPFDGIVYNIQLTQGDYENQVIETASNMAASF
                     VAKITKIEKQAPSGNGGKTTDPQNPSGQQTSTDPEKKPTGGNEPVGKPDVEVPNKLPT
                     GESFENDYFKIILPKNWTVEKTTDMLITLFPPDKTQGSIMIATQTGNTEKAENIAKIM
                     TSSIEGSTTPVSVQLGSGIGYQFVYSDGGIKQVQTVYTKDDKYYAITYVQNKQGVDLK
                     QDYDSLLLSLFAK"
     gene            complement(1634..>2153)
                     /locus_tag="PK717_07405"
     CDS             complement(1634..>2153)
                     /locus_tag="PK717_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47521.1"
                     /translation="QEKLDLVKMAIESFKITKPDYFTKKEPEKKDTETTPKTEETKFG
                     QPLVAEKITITPLEGWTSKYEKLTSTITLTNAANRTFSMVILDRQGMKKDDYTKSLRE
                     GLKQSADLKVKESTEKIGEIEYDRIDFLTMDGRTAMTDLVGEKNNIMIVIMIQDGLSS
                     DIQKMLETIKTN"
BASE COUNT          467 a          439 c          484 g          763 t
ORIGIN      
        1 gtcaccttgt attggtgttg tctccagatc aagctctttg ccgattttgc cagttaatgg
       61 atcataaaca tagagaaagc tgtttgtcga ttttggggaa gtccttatcc tgacggcctt
      121 gtctaccaga acgggtttta ttgagtctga ttcaacaggt tcttttgaga ggctgattac
      181 aaatctgtct ccctgccctt ttttgtacag gatttcttcc tgtgctggca ggtaccccgt
      241 tacctttgct tctatcttgt ggacaccttc ttcaagctca agctgtgctg gagaagtata
      301 ttccttgcct tcaaagatga ttgttccacc ctttgggttt gtctctatgg taactgcccc
      361 gccgccacag cctgcaagaa acagtatggc gaggattatc gccgggaatt tatttagcaa
      421 agagcgatag gagaaggctg tcataatcct gcttaagatc aacaccctgt ttgttctgga
      481 cataggttat agcatagtat ttgtcgtctt ttgtgtaaac cgtctggacc tgttttattc
      541 cgccatcgga gtagacaaac tggtaaccga tgcctgagcc aagctgtacg gaaacgggag
      601 ttgtgcttcc ttcaattgag gaggtcatta ttttggcgat gttctctgcc ttttctgtgt
      661 tgccagtctg agtggcaatc atgatgctcc cctgtgtctt gtctggcggg aaaagggtga
      721 tgagcatgtc cgttgttttt tctactgtcc agttttttgg gaggattatc ttgaaataat
      781 cattttcgaa agactcgcca gttgggagct tgttcggaac ttcaacatct ggtttgccta
      841 ctggctcatt gccgcctgtc ggcttttttt ccggatcggt agaggtttgc tggccagatg
      901 ggttttgcgg gtctgttgtt ttgccaccgt ttcctgatgg tgcttgcttt tctatttttg
      961 ttatttttgc aacaaagctt gctgccatgt tgctggcggt ttcgatgacc tgattttcgt
     1021 aatcgccctg ggtgagctgg atgttataga caatgccatc aaacgggaac agggctatta
     1081 ctgattccgg attttgtgtt tcaatccacc atattttatt tttttcaagc tctactggtt
     1141 catttttgat tattttgcct gtcagtgtct gggtaagcga ggaaacgagc tgatttatcg
     1201 agcttgtgtc gccagttttt gatgaggcat cgggggcgta agttatagaa aattcgacat
     1261 tctctgattt tataaacacc acgttgtctt tctctgccac ctggtataaa gacgggtagg
     1321 cgattgaaag atggctgttt tcaaaggttt tgtccagcgc aggcgcagat ttgccgcacg
     1381 aagaaagcat gacgcagact gatataaaga ccaaagcggg tgtgttatgt aatcttcttg
     1441 atatgttcat cattgttctc caagagacct attttgcaat catagctata tatgtcaaca
     1501 tcgtatttta tccaaacaat taagtgatgg tttagtcgca tcaatacatc atacatccca
     1561 tatcgtgatt tttatccata aaaaatgccc cctcaaaaga gagggcattt tgttgcagca
     1621 gggaaattgt ggcctagttt gtttttatag tttcaagcat cttttgtatg tcgcttgaga
     1681 gaccatcctg gatcattatc actatcatga tgttgttttt ttctccaaca aggtcggtca
     1741 tagcggttct gccatccatt gtcaaaaaat caatcctgtc atattcgatt tcaccaattt
     1801 tttcggttga ttcttttacc ttcaaatcag cactttgctt aaggccttct cttaagcttt
     1861 ttgtatagtc gtcctttttc attccttggc gatcaagtat caccatcgag aacgttctgt
     1921 tggccgcatt tgttaatgtt attgtactgg tgagtttttc atatttggaa gtccaaccct
     1981 caagcggtgt gattgtaatt ttttctgcca caagcggctg gccaaatttt gtctcttcgg
     2041 tttttggagt ggtttcggtg tccttttttt caggctcttt ttttgtgaag tagtcaggtt
     2101 ttgttatctt gaaggactca atggccattt ttacaagatc gagcttctcc tgc
//
LOCUS       DAOSRI010000276         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_46839_length_2147_cov_4.687380, whole genome shotgun sequence.
ACCESSION   DAOSRI010000276 DAOSRI010000000
VERSION     DAOSRI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46839_length_2147_cov_4.687380"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PK717_07410"
     CDS             <1..580
                     /locus_tag="PK717_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR47522.1"
                     /translation="EAVKAKERLDFLNGQLSDLAHTEANLRSSLSEVETRIKKTFEET
                     YRSVESHFRTLADVLFAGAIGNLRRITGENGQTEGIEVEFILPGRKLKTLHALSGGEK
                     TLAALALLFAFFKTKSSPFCVLDEVDAALDDSNIERFTRLLRNEAEQTQFVVITHNKE
                     TMRWCDALYGITLDTTGTSRVVSVKLDGVENS"
     gene            631..1458
                     /locus_tag="PK717_07415"
     CDS             631..1458
                     /locus_tag="PK717_07415"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOR47523.1"
                     /translation="MKSIKREGLVDLVASLCIKANLVLPKYQKEKLEWALENETSPLG
                     KKLISQIIENYKIAEKTGLAICQDCGLAVVFLEAGRDVHLDFDIYEAIKDGVAKGYSE
                     GHLRPSVLHSCVDRTNTKTNTPAIIHTQIVDGDKITLTVTPKGGGSENASRLAMLKPY
                     EGKQGIVDFVVKTVRENGPSACPPVTVGIGFGGTIEECALLAKKALLAENRSEELGDM
                     EKEILELCNNLGFGPAGLGGKISVLDVHILSTGCHIATMPVAVNIQCHAARKATGEI"
     gene            1455..1985
                     /locus_tag="PK717_07420"
     CDS             1455..1985
                     /locus_tag="PK717_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOR47524.1"
                     /translation="MKEHRLTTPLSKKSLANLACGDEVLITGTIYTARDAAHKAMLKM
                     MQEGRELPFDPRGAIIYFAGPSPAPPGFPIGSCGPTTSSRMDPFTRTMFELGIAAQLG
                     KGPRGQEVTKTLVDFGGVYLVALGGAGALLAKKVKKAKLLAFPELGPEAVYELEVEDF
                     PAIVAIDSKGNNIFKR"
BASE COUNT          637 a          497 c          547 g          466 t
ORIGIN      
        1 cgaggcggtg aaagccaaag aaagactgga ttttctgaac ggccagctct ccgatctcgc
       61 acataccgaa gcaaacctga gatcgtcgct atccgaagtg gaaacgagga taaaaaaaac
      121 tttcgaagaa acctaccgct cggtggagag ccacttcagg acgcttgcag acgtactctt
      181 tgccggtgca ataggcaatc tgcgcaggat caccggggaa aacggacaaa ccgaggggat
      241 tgaggtcgaa tttatcctgc caggcagaaa gctcaagaca ctccacgccc tttcaggggg
      301 tgagaaaacc ctggccgccc ttgcattgct gtttgccttc ttcaagacaa aaagctcgcc
      361 gttttgcgtc ctggacgagg tggatgcggc ccttgatgac agcaacatag aaagatttac
      421 gaggttgctc cggaatgagg cagagcaaac gcagttcgtt gtcataacac acaacaagga
      481 aaccatgcgc tggtgcgatg cgctttacgg cataacgctt gacaccaccg gaacctcaag
      541 ggtcgtttca gttaagcttg atggtgtcga aaacagctga tcacagtttg ctggcttgca
      601 aaaagccaaa agatgctatc atttgacttg atgaagtcga taaaaaggga agggctcgtt
      661 gatctggttg ccagtctgtg catcaaagcg aatctcgtgc tcccaaaata ccaaaaagaa
      721 aaactcgaat gggcacttga gaatgaaaca tccccgcttg ggaaaaaact catatcgcag
      781 ataatcgaaa actataagat cgctgaaaaa accgggctgg caatctgcca ggactgtgga
      841 cttgcagttg tttttcttga ggcaggcaga gatgttcacc ttgattttga catctatgag
      901 gcaataaaag atggcgttgc caaagggtat agcgaaggcc atttgaggcc atctgtcctg
      961 cattcgtgcg ttgaccgaac aaacacaaaa accaacactc cagcaataat ccacacacaa
     1021 attgttgatg gtgacaaaat aacgctcact gtcacaccca aagggggtgg ctccgaaaat
     1081 gcaagcaggt tggcaatgct caaaccatac gaaggaaagc agggcatcgt cgattttgtt
     1141 gtcaaaacag tcagggagaa tggtccttcg gcctgtccac cagtaacagt cgggataggt
     1201 ttcggtggaa caattgaaga gtgcgccttg ctggcaaaaa aggccctgct ggcagagaac
     1261 cgatctgaag aattgggtga catggagaaa gagatactgg aattgtgcaa caatcttggc
     1321 ttcggcccgg cggggcttgg cggcaaaatt tcagttctgg atgtgcacat actatcaaca
     1381 ggctgtcata ttgcgacaat gccagttgca gtaaacatcc agtgccacgc cgcaagaaag
     1441 gcaacgggtg agatatgaag gagcacagac taacaacccc gctctcaaaa aaatccctcg
     1501 ccaatcttgc ctgtggtgat gaagttctga tcactggaac aatctacaca gccagagatg
     1561 cggcccacaa agccatgcta aaaatgatgc aggaagggag ggaactcccg tttgatccaa
     1621 gaggggcaat aatctacttt gcaggcccgt caccggcacc accaggtttt ccgattggat
     1681 cctgcgggcc aacaacatct tcaagaatgg accctttcac aagaacaatg ttcgagcttg
     1741 gcattgcagc acagctcggg aaggggccaa gggggcaaga ggtcacaaaa acccttgttg
     1801 atttcggtgg tgtttatctc gtggcactcg gaggggcagg ggcactgctt gcaaaaaagg
     1861 tgaagaaagc gaaactgctt gcatttccag agcttggtcc agaggcagtc tatgaactgg
     1921 aagtagagga ttttccagct atagttgcaa tagatagcaa aggaaacaat attttcaaaa
     1981 gataatattt ccttcaaaaa taaaaacggg ccagcacaaa actggcccat cttattttct
     2041 actggttttt tttatttgct acttgccaag ttgctcaatc agctgcgtaa gggtctcaac
     2101 acagtccccg acaatgccgt aggaggctac cttgaatatt ggcgcat
//
LOCUS       DAOSRI010000277         2138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_47135_length_2138_cov_3.114738, whole genome shotgun sequence.
ACCESSION   DAOSRI010000277 DAOSRI010000000
VERSION     DAOSRI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47135_length_2138_cov_3.114738"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK717_07425"
     CDS             complement(<1..387)
                     /locus_tag="PK717_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOR47525.1"
                     /translation="MKSVLVTADDFGFTKSVTDGVLKAHDEGIVTHTGLMVNMPDAQR
                     AVELAKLRPSLSVGLHFNLTQGKPISDPKLIPSLVRENGEFKGLSLWYPRRANPKDIE
                     VELRAQIEKFMSFGFDKIHIDGHHYVT"
     gene            complement(449..1384)
                     /locus_tag="PK717_07430"
     CDS             complement(449..1384)
                     /locus_tag="PK717_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47526.1"
                     /translation="MDKQHIELFTNLDGNFHDSFTEALSTTLNINGIITSTGFLAGLT
                     GTAFAPACNTDEECTAWWTESDCIDHRLDFIKDAISLDIKTFNLGKGIWPIPEKLPEE
                     ALLHIANEKAVLLKSWPVWQIAANTDGKAEKIAFKGFEGLDWCENCFKNCFIITAKAR
                     SSDEKCTVLEAIKHGAKLATGNFSIGTTFWGTALYDKAIEKLEEEYFCPSCKEESIGC
                     AYRTFRRIEGTIFYGKSFTNEIKKLGLIKGQISNELTSTLETMSKVAEKLTSKGFRER
                     YTSDSFASDSKISLLELKKGQEKIGELWTKAALSI"
     gene            complement(1398..1817)
                     /gene="dut"
                     /locus_tag="PK717_07435"
     CDS             complement(1398..1817)
                     /gene="dut"
                     /locus_tag="PK717_07435"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005509732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR47527.1"
                     /translation="MKIKKLRQDAILPQRAHDTDAGLDLTTPDEVELLPLERKLVMLG
                     FSMSIPDGMVGLVCPRSGLAAKNGITVLNAPGIVDSGYRGEVGVVLINLGKDKTVLPK
                     GSRIAQMVIVSYNDMQPVWAENLDETERGQGGFGSTS"
     gene            complement(1817..>2138)
                     /locus_tag="PK717_07440"
     CDS             complement(1817..>2138)
                     /locus_tag="PK717_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47528.1"
                     /translation="TGRGGKGGANDASAEFMGAIRKLLSDNNIVFQTAELGAVDIGGG
                     GTVAMYFAARGIDTIDAGPAVLGMHAPKETISKADLFSTYQAYKTFLDKFAGEYAKIA
                     KNTN"
BASE COUNT          502 a          503 c          461 g          672 t
ORIGIN      
        1 agttacataa tggtggccat caatgtggat tttgtcgaaa ccaaatgaca tgaatttttc
       61 gatttgggcc ctgagctcta cttcgatgtc ttttgggttg gctcttcgcg gataccagag
      121 tgaaagtcct ttgaattcac cattttcacg gacgaggctt ggtataagtt ttgggtctga
      181 gatgggcttg ccctgggtca gattgaaatg gagtcctaca gaaagcgaag gccttaattt
      241 tgccaactca accgctcttt gggcatctgg catgttgacc atcaagcctg tgtgggttac
      301 gattccttcg tcatgtgcct ttagaacacc atctgtaaca gattttgtga atccaaaatc
      361 atcggctgtg accaggacgc ttttcattgc aggctatttt agcaaagaaa aataacaatc
      421 aaagggtcaa aatatataca ccctctaatt agattgataa agcagctttt gtccatagct
      481 caccaatttt ctcctggcct tttttcagct ccaggagtga tatttttgaa tctgatgcaa
      541 aactgtcgga agtatatctt tccctgaagc ccttgcttgt caatttttct gcgaccttgg
      601 acatagtttc aagagtgctt gtcagctcat ttgaaatttg accttttatt aaaccgagtt
      661 ttttaatttc gttggtgaaa gattttccgt aaaaaatggt tccctcaatc cttctgaatg
      721 ttctgtatgc acagccaatg ctctcttctt tgcaggaagg gcaaaaatac tcttcttcca
      781 gcttctcgat cgctttatca tagagggctg tcccccaaaa agttgtgcca attgaaaaat
      841 tccccgtggc cagctttgca ccatgcttta tggcttcaag tacagtgcat ttttcgtcgg
      901 aggatcttgc ctttgctgta ataataaaac aatttttgaa acagttttcg caccaatcaa
      961 gtccttcaaa tcccttaaac gctatttttt cagcctttcc atctgtgttg gctgcaattt
     1021 gccacactgg ccatgattta aggagaactg ccttctcgtt cgcaatgtgt agcaatgctt
     1081 cttcaggcaa tttttcaggg attggccata ttccttttcc caggttgaaa gtttttatgt
     1141 ccagactaat cgcatctttg ataaaatcga gcctgtgatc aatgcaatca gattccgtcc
     1201 accatgcagt acattcctcg tcggtattgc atgcgggggc gaatgctgtg cccgtgagtc
     1261 ctgccagaaa acctgtgctg gtgataattc cattgatatt taatgttgtg gaaagtgctt
     1321 ccgtgaatga gtcatgaaaa ttcccgtcaa gattagtgaa caattcaata tgttgcttgt
     1381 ccaaagtaac ttcctcctca ggaagttgaa ccgaaaccgc cttgccctct ctctgtttca
     1441 tcaaggtttt cggcccacac cggctgcatg tcattgtatg atacaatgac catctgggca
     1501 atccttgaac cttttggaag cacggttttg tctttcccaa gattgatcag cacaacccct
     1561 acttcacccc tgtatcccga atcaactatt cctggtgcgt tcaatacggt tatgccattt
     1621 ttggccgcaa gccctgagcg ggggcaaacg agaccgacca tcccgtcggg aatggacatt
     1681 gaaaatccga gcatgacaag ctttctctct aaaggcaaaa gctcgacctc atccggtgtg
     1741 gtgagatcga gccctgcgtc cgtgtcatgt gctctttgag gaagtattgc gtcctgtctg
     1801 agctttttga ttttcatcag ttggtgtttt tagctatctt tgcatactcc ccggcgaatt
     1861 tgtcgaggaa ggtcttgtat gcctggtagg tgctgaagag gtctgcctta cttatagttt
     1921 cttttggtgc gtgcattcca aggactgccg ggccagcatc gattgtgtct atccctcttg
     1981 ctgcgaagta cattgcaaca gttccaccgc caccaatgtc gacagcgccg agttctgcag
     2041 tttggaagac aatgttattg tcagagagga gcttccttat tgcgcccatg aattcggccg
     2101 atgcgtcgtt tgctccacct tttccacccc tgccggtg
//
LOCUS       DAOSRI010000278         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_47822_length_2116_cov_3.338185, whole genome shotgun sequence.
ACCESSION   DAOSRI010000278 DAOSRI010000000
VERSION     DAOSRI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47822_length_2116_cov_3.338185"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK717_07445"
     CDS             <1..189
                     /locus_tag="PK717_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47529.1"
                     /translation="VIFLWVGRDYAISGGTMVQLDAPPIIQGGRTYIPVRFVSEQLGA
                     QVTWDGKNRIVTVKLKMD"
     gene            complement(186..1655)
                     /locus_tag="PK717_07450"
     CDS             complement(186..1655)
                     /locus_tag="PK717_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47530.1"
                     /translation="MDESGNPMSWEEVKMMGCARMRFEMKHGAPGPRPIIMPGHCPNE
                     YFETTGNIADIGEGIATVQTPQGPMQVRFIEGETAMMGEIGEGMPIRVKGLRFGPRVI
                     ARFVAPMDENKNGQSQPKSPKDGGSNTQAPSSGNKTGENKPTPKSPANSGTKDTSKKT
                     GTGNKNQSGTPDYLNSTFETTLKNISETKITTDVGQFLINQNTLVTVKFDGNEFALHP
                     KILSRIAKNTKLTVSTKNGVSTAILITNTGFSIKKLWMIKDDFRNMRLLCVDETLAEK
                     IVQLIPETLASDFDGIQIPSQEIFQEIRPGNTLLVLEYKNQALHIAHADKPQPPEEIR
                     GEISEKTGSFIVINGKQINIISSTECECAGKGKGSCSRNEINVGDKAVAIVFKINGKI
                     VATKVVKMPKGMGGMGMGQGDIKKLPKFQIESLTRTQNGIQITFKDGPVLLVTPKTAI
                     KIIKNGTEERPGSPEDLKTGMMVGVEFSNRQAIQIIIEP"
     gene            1663..1998
                     /locus_tag="PK717_07455"
     CDS             1663..1998
                     /locus_tag="PK717_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOR47531.1"
                     /translation="MSSGTGIGPPKFIGTIVWSSTSALISSSEASRIIRFLSIIGKAI
                     AGSTEKTDTQSDTSGKYLYYTLKKGDTLWEIAKKYEGVTDRDLLRINGLTDGKSLKPG
                     QQIKIKRLD"
BASE COUNT          492 a          450 c          462 g          712 t
ORIGIN      
        1 gtcattttcc tctgggttgg cagagactat gcaatatcag gcggaacaat ggttcagctt
       61 gatgcgcccc caataatcca gggaggcagg acttatattc cagtgagatt tgtctcagaa
      121 cagcttggtg cccaagttac ctgggatggg aagaacagga tagtaacagt taaactgaag
      181 atggattagg gctcgataat tatctgaatg gcctggcggt ttgagaattc aaccccaacc
      241 atcatgcccg tcttgaggtc ctctggcgag cctggtcttt cttcggtacc atttttgatg
      301 atttttattg cagtttttgg ggtaactagt aacactggcc catctttaaa tgtgatctga
      361 atcccatttt gggtgcgagt caacgattcg atttgaaatt tggggagctt tttaatatcg
      421 ccttgcccca tgcccatacc acccatgcct tttggcatct tgacgacctt tgttgccaca
      481 atcttgccgt ttattttaaa tactattgcg acagctttgt ctcccacatt gatttcattc
      541 cgtgagcagg agcctttccc tttcccggcg cactcgcact cggttgaaga tattatgttt
      601 atctgtttgc cgtttatgac aataaaggac ccggtttttt ctgatatctc tccccttatt
      661 tcctcaggtg gctgtggttt atcggcatgt gcaatatgca gggcctggtt tttgtattcc
      721 agaaccaaca gggtatttcc gggccttatt tcttggaata tttcttgaga tggtatctgg
      781 atgccatcga agtcggatgc caaggtttct ggtatgagtt gaactatttt ttctgcgaga
      841 gtttcatcca cgcacagcaa tcgcatgttt ctaaaatcat cttttatcat ccagagtttc
      901 ttgattgaga atcctgtgtt tgtaatgagg atggctgtcg aaactccatt tttggtcgac
      961 actgtcaatt tcgtgttttt cgcaatcctt gaaagtatct ttggatgcaa ggcaaattcg
     1021 tttccatcaa actttacagt taccagggtg ttttggttga tgaggaactg gcctacatct
     1081 gttgttatct ttgtctcgct gatgtttttg agcgttgttt caaaagtcga attcaggtaa
     1141 tcgggggtgc cagactggtt cttgttgcca gtgccggttt ttttggatgt atcttttgtt
     1201 ccgctatttg caggagattt tggtgttggt ttgttttctc cagttttgtt cccactgctc
     1261 ggtgcttgag tattgctacc accatctttg ggacttttcg gttgcgattg cccgtttttg
     1321 ttttcatcca ttggtgcgac aaaacgtgct atcactctgg ggccaaacct tagccccttg
     1381 acccttatcg gcattccctc gcctatttca cccatcattg cggtttcacc ctcaatgaat
     1441 cttacttgca tcgggccttg aggggtctgg actgtggcaa ttccttctcc aatgtctgct
     1501 atattgccag ttgtttcaaa atactcgttt gggcagtgcc ctggcatgat gatgggcctt
     1561 ggtcctggtg cgccatgttt catctcaaag cgcattcttg cacatcccat cattttgact
     1621 tcttcccagg acatgggatt tccactctcg tccataggag atgtgtcttc tggcactggt
     1681 attggcccgc caaaattcat tggaactatt gtatggtcaa gtacttctgc gttgatctca
     1741 agttctgaag catcgagaat cattcgcttt ctctcgatta ttgggaaagc tattgccggg
     1801 tctacagaaa aaactgatac tcaaagcgat acatctggaa aataccttta ttacactcta
     1861 aaaaaaggcg acaccttgtg ggaaattgca aaaaaatatg aaggtgtaac agatcgtgat
     1921 ttattgcgta ttaatgggtt aactgatggg aaaagtttaa aacccggtca gcagataaaa
     1981 attaaaagat tagactaaca gaagaatatt taactctttg taacacctaa aactaatgaa
     2041 ccaaaacata aaaccatctt atgttttttg gacaacagta gtaacattgc tgattttagg
     2101 ttatgtatcg tttctt
//
LOCUS       DAOSRI010000279         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_47904_length_2113_cov_5.627308, whole genome shotgun sequence.
ACCESSION   DAOSRI010000279 DAOSRI010000000
VERSION     DAOSRI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47904_length_2113_cov_5.627308"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK717_07460"
     CDS             <1..293
                     /locus_tag="PK717_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR47532.1"
                     /translation="CPYITEEIWELLGNKASVHQQPWPVGDETLTKEDTINIPIQING
                     KLRHVIQIQIGTSEEEIQKIALSEEQVKKFTDGHEILKIMVPKGKLVSIVIK"
     gene            389..1804
                     /locus_tag="PK717_07465"
     CDS             389..1804
                     /locus_tag="PK717_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOR47533.1"
                     /translation="MKRSVAVGIVIFISFFVINVLALSSNTLKFTLGEKSVSVGEESV
                     EMNVPLFKIGDEHFVSMEFLAKHLKGTSIQLDGNNVSFSVGEVTEDPGAGGEGGEELL
                     DIMTSLHEKINKHRAEIGAPPLEISMPATFAAQDHTTDMCIREFFDHTNPDGLKPGDR
                     LSNYGVKWSKYGENIQKLTIVDDIASKIDENFQNSPNHKKNRLDKDFTHIGIGLYKCG
                     NEMFVTELFFTPREQTKPTDKGELKFFDATYWETSESGDSSKIVACFKNTGKTNLTNI
                     TVMFNVLDSKEEVIATKILSFYGILEQGQYFTDSFTTEGGISIPDDAKVSYEIEFEET
                     SESNPKVEIIESGIEKGKDFVAAKGKITNKSSEKSFVFIIPTFFSEEGRNLRIADYGI
                     CEIESFEPGKTYDYTALSEGTPDFVSKHVSKTDYSYWFIPRENLPMAHNMEKVSGWKM
                     PYHKLFKNGLKTAYLKNGSTK"
     gene            complement(1883..>2113)
                     /locus_tag="PK717_07470"
     CDS             complement(1883..>2113)
                     /locus_tag="PK717_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOR47534.1"
                     /translation="ECRAWTIRKGDNAVTAAGKVHSDIAKGFIRAECCTYEDLLASDN
                     SLQKAKANAKLRLEGKEYIVKDGEICHFRHSG"
BASE COUNT          703 a          449 c          449 g          512 t
ORIGIN      
        1 tttgcccgta tatcacagaa gaaatttggg aattgttggg gaataaagca tctgttcatc
       61 agcagccatg gcctgtgggt gatgaaaccc tcacaaaaga agacaccatc aatatcccaa
      121 tacaaatcaa cggcaagctc cgccacgtca tccaaattca aattggaaca agcgaagagg
      181 agatccaaaa aattgcactc tccgaggagc aggtcaaaaa gttcaccgat ggtcacgaga
      241 tactgaaaat catggtccca aaaggaaaat tggtcagcat agtgataaag taaccacaaa
      301 aacaaaagcc ctgcaacaag cagggctttt ttaatagtat tatttactat acgggtcttt
      361 tgggctagaa tgcatgtgga ggtgcccctt gaaacgaagt gtggccgttg gaatcgtgat
      421 tttcatatca ttttttgtca tcaatgtgct tgccctgtca tcaaatactc ttaagttcac
      481 gcttggcgaa aaatcggtat cggttggcga agagtctgtc gaaatgaatg ttcctctctt
      541 taaaatcgga gatgagcatt ttgtttccat ggaatttttg gccaaacacc tcaagggcac
      601 atcaatccag ctggatggaa acaacgtgtc cttctctgtt ggcgaagtaa ccgaggaccc
      661 tggcgcaggt ggtgaaggtg gagaagaact gcttgatata atgaccagcc ttcacgaaaa
      721 aataaacaag cacagagcag agattggagc tcctccactc gaaattagta tgccggcaac
      781 atttgcagca caggatcaca caacagacat gtgcataagg gaattttttg accacacaaa
      841 tccagatgga ctgaaaccag gcgacagact cagcaactac ggagtaaaat ggtcaaaata
      901 tggtgaaaac atacaaaaat tgacaatagt tgacgacatt gcgagcaaaa tagacgagaa
      961 tttccaaaat tcacctaacc acaaaaagaa caggctggac aaagacttta cccacatagg
     1021 gattggtctg tacaaatgcg gaaatgaaat gttcgttacg gaactgtttt ttacaccaag
     1081 ggaacaaaca aaaccaactg acaaaggcga gttgaaattt tttgatgcaa cctattggga
     1141 aaccagcgaa agtggagatt ccagcaaaat agttgcttgc ttcaaaaaca caggaaaaac
     1201 aaacctcacc aacatcacgg tcatgtttaa tgtgctagat tcaaaagaag aagttattgc
     1261 aacaaaaata ttgagctttt atgggatact cgagcaggga cagtatttta cagattcctt
     1321 tacaacagaa ggtgggattt caattcctga tgatgcaaag gtgtcatacg aaatagagtt
     1381 tgaggaaacc agcgagtcaa acccaaaagt ggaaatcata gaatctggga tagagaaggg
     1441 caaagatttt gtggctgcaa aagggaagat aacaaacaaa tcatctgaaa aatcctttgt
     1501 gttcattatc ccaaccttct tcagtgagga gggcaggaac ctcaggatag ccgattatgg
     1561 aatatgcgag atcgagtcat tcgaaccagg taaaacatac gattatactg ccttatcgga
     1621 gggtacacct gattttgtgt ccaaacacgt ctccaaaacg gactactcct actggttcat
     1681 cccgcgtgaa aacctaccca tggcccacaa tatggaaaaa gttagtgggt ggaagatgcc
     1741 ataccacaaa ctcttcaaaa acggcctaaa aactgcatat ctcaaaaacg gctcgaccaa
     1801 ataaaaattt tctatctttc taaaaacaaa aaagcctgac cattaggtca ggctttttaa
     1861 ttatcacaga ttcaggacaa gattagccac tgtgcctgaa atgacagatc tcaccatctt
     1921 tgactatgta ttctttgcct tcaagcctga gtttggcatt cgcttttgcc ttctggaggg
     1981 aattgtccga agccagcaag tcctcatagg tgcaacattc agccctgatg aatccctttg
     2041 ctatatcgct gtgaacttta cctgcggcag tgacagcatt gtcgcccttc ctgatcgtcc
     2101 aggccctgca ctc
//
LOCUS       DAOSRI010000280         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_48296_length_2101_cov_4.809384, whole genome shotgun sequence.
ACCESSION   DAOSRI010000280 DAOSRI010000000
VERSION     DAOSRI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48296_length_2101_cov_4.809384"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PK717_07475"
     CDS             complement(<1..786)
                     /locus_tag="PK717_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOR47535.1"
                     /translation="MEIGIIGAPQSGKSTLFTAITGVEPGNEVGKRAVVKVPDPRLDH
                     LTDVFKPKKKINATVNFIDAPPPQSMSAKRGSAPSFLGFIKTVDAILVVAACYRDGGA
                     KELQDIMEMLLLSDLEVIEGILARENKIKIDPKYSGPSMELLRKLAGELEQMKMLRNI
                     ELSEKELASLSGVQLFTKYPTFVALNISENDIKGELKGEAKKAADYLDSIKTPYIAVC
                     APIEAEIATLPDEDRESFMQEYGLESTASDRVIQEVYDLLGYQT"
     gene            complement(821..1744)
                     /locus_tag="PK717_07480"
     CDS             complement(821..1744)
                     /locus_tag="PK717_07480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR47536.1"
                     /translation="MNLEYNLEERQRLDVFLTSKLPFLSRTQIAKMIKDGLVTVDGDE
                     AKPSYNLKPGEQIDIELPTPEPYELEPENIPIEIVHEEDDFLVAVKPQGMLTHPAGAT
                     ITGTLVNAMLYYCKGKLSGMVGEQRPGIVHRLDRDTSGLIIVVKTDYAMKMFAKLFAE
                     HRMDKRYYCICKGHLKSERTVINAPIARDFTHRWKMCVSPDGREAISKITELERFDGY
                     TYAEVKPVSGRTHQIRVHLSYIGHPILGDQVYGGEDSRFDLKGQLLHCGKLGFDFKGV
                     HYELEAKPPPIFLETLEKLRLTGGMQDLNSK"
     gene            complement(1745..2056)
                     /gene="cutA"
                     /locus_tag="PK717_07485"
     CDS             complement(1745..2056)
                     /gene="cutA"
                     /locus_tag="PK717_07485"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOR47537.1"
                     /translation="MSLFLVYCPYPTKSQAEQAAKNAVYKKLAVCANIFQSDSIYEWK
                     GELVHDSERVVIFKTIEQKVEELKKFVEENHPYELPAIITFGPSDVNLPYLEWAKGQL
                     L"
BASE COUNT          501 a          545 c          455 g          600 t
ORIGIN      
        1 ggtctgatag ccaagcagat cgtaaacttc ctgtatgacc ctgtccgagg ccgtagattc
       61 gagcccatat tcctgcatga agctctccct gtcctcatct ggcaatgtgg ctatttctgc
      121 ctcaatcgga gcacatacgg caatgtatgg tgttttgatt gagtccagat aatcagcggc
      181 cttctttgct tcgcccttca gctcaccttt tatgtcattc tctgaaatat tgagtgcaac
      241 aaatgttgga tatttcgtaa aaagctggac gccggaaagc gaagccagtt ctttttcact
      301 gagctcgatg tttctgagca ttttcatctg ctccagttcg ccagccagtt ttctgagcaa
      361 ctccatggat gggcctgaat acttggggtc aatttttatc ttgttttccc tggcaagaat
      421 gccttctatc acctcaaggt cagacaaaag aagcatttcc attatgtcct ggagttcttt
      481 ggccccacca tccctgtaac aggccgcaac aaccagaatg gcgtcaacag tttttatgaa
      541 gccaagaaaa gatggcgctg aacccctctt tgccgacatg ctttgtggtg gcggtgcatc
      601 aataaaattc actgtggcat ttattttctt ctttggtttg aaaacatcgg ttagatggtc
      661 gagcctcgga tcagggactt tgacaaccgc cctcttgcca acctcattgc caggctcaac
      721 acctgtgatt gcggtaaaaa gggtgctttt cccagattgc ggagcaccaa ttattccaat
      781 ttccatttga atcaaacctc cattcttgtg gagaaacaga tcactttgag tttaaatcct
      841 gcatgccacc agtcaagcgc aatttttcaa gggtttcaag aaatatcggt ggtggcttgg
      901 cctccagctc ataatgcacc cctttgaaat caaaccccag cttgccgcaa tggagtaact
      961 ggccctttag gtcaaatctg gagtcctccc cgccataaac ctgatccccc aaaatggggt
     1021 ggccgatata tgaaagatgt acccttatct ggtgcgtcct tcctgaaaca ggcttgactt
     1081 cagcataggt atacccatca aacctttcca gttcggtaat tttcgatatc gcttctcttc
     1141 catccggaga gacgcacatc ttccacctgt gcgtaaagtc ccttgctatc ggggcattta
     1201 tcacagttcg ctccgatttc agatgaccct tgcatatgca gtagtatctc ttgtccatcc
     1261 tgtgctcagc aaaaagcttt gcaaacatct tcattgcata gtcggtctta acaacaatga
     1321 tcaatcctga agtgtccctg tccagcctgt ggacaatccc ggggcgctgc tccccaacca
     1381 tgcccgaaag tttgcccttg cagtaatata gcatggcatt gacgagtgtc cccgtgattg
     1441 ttgcaccagc cgggtgggta agcattccct gtggcttgac tgcaaccaga aaatcgtcct
     1501 cttcgtggac aatctcaata ggtatgttct ctggttcgag ctcatagggc tccggcgtcg
     1561 ggagctcaat atctatctgc tctcccggct tcagattgta ggaaggctta gcctcgtcac
     1621 catcaacagt gacaagaccg tccttgatca tttttgcaat ttgggtcctg gaaagaaacg
     1681 ggagctttga cgtaagaaac acatcaaggc gctggcgttc ttccagattg tattcaaggt
     1741 tcacttacaa aagctggcct ttcgcccact caagataggg caaatttaca tcggaaggcc
     1801 caaaagttat tatggccggc aactcatatg ggtggttttc ttcaacaaac tttttcagct
     1861 cttccacttt ctgctctatt gtcttgaaga tgaccacgcg ctcagaatca tgcaccagct
     1921 cacccttcca ttcgtagatt gaatccgact ggaagatgtt tgcgcacacg gctagcttct
     1981 tgtaaacagc attttttgcc gcctgctctg cctgtgactt tgttgggtag ggacaataaa
     2041 ccagaaacag gctcatggtg tcgggttagg gtaagttatc acaaccatcc ttgcttcctg
     2101 a
//
LOCUS       DAOSRI010000281         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_48640_length_2090_cov_4.138575, whole genome shotgun sequence.
ACCESSION   DAOSRI010000281 DAOSRI010000000
VERSION     DAOSRI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48640_length_2090_cov_4.138575"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PK717_07490"
     CDS             complement(<1..1156)
                     /locus_tag="PK717_07490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR47538.1"
                     /translation="MKLMPFALTILLAICSLGCGMTNENAGSGENLFITTLPADVIYN
                     ATRDVKLGIESDFLLKAVKIANDGKEVIEVKSMTFTIFANEREIFSLIYKGEELNSLF
                     GKYVEKFEKMTYDFQEGIFLGTSGFVDLKKTSKSVVIEPGQEAAIINIPFRFYSDNPA
                     TKCNIHIEFQKGNKTFSRDLGLQVAEYVLKNKYIFPAKGIWMAINTYNANYGHRVNSS
                     QEFAFDLMIQKPNLMMYPPSGKNEDYGCWGKAVIASADGVVVDCVDGVPNNPPYLGSR
                     LSKEELEKVMEKFGAVAMAGGNYVILEHSGSEFTFYAHLINGSVKVKKGDIVKQGQVL
                     GLCGNSGNSDAPHLHFHIMNGPSVLDSRGLPMKFTNLKDFVGGKIEQITDP"
     gene            complement(1264..>2090)
                     /locus_tag="PK717_07495"
     CDS             complement(1264..>2090)
                     /locus_tag="PK717_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HOR47539.1"
                     /translation="PTAPRSFGFSAIVEETPVDSQINNQAFSRHALDQGVPALSNNVA
                     VNTKKPVKKAILEVTKKAKAFQYNIDDIFAFQITIRNIGNLDATNVVLDDVFPRELKY
                     VSSLPSGAAGPSKIRYQVGSLSPGQSKTYLLKFSLAKSLSFAGNCLTITNKASAVSDS
                     AEAVTDYATISVCKQPPTKEIVLDATWKGINTKTNEARVNEEIKLDLSVEGGAPPYEI
                     TVNWGNGTKSTKTIQSKDNLVSFENSYSSQGDFEVLITCVDSFGRSARQSRVIHIK"
BASE COUNT          539 a          486 c          442 g          623 t
ORIGIN      
        1 tcgggtctgt tatttgttca atcttgccgc ctacaaaatc cttgaggttt gtgaatttca
       61 tgggcaatcc ccttgaatca agtaccgacg ggccattcat gatgtgaaaa tgcaggtgtg
      121 gagcatccga gttgccagaa ttgccgcaaa gtcccaatac ctgtccctgt ttcacaatgt
      181 cgcccttttt gaccttcacc gagccattta tcaagtgggc gtagaacgtg aactcgcttc
      241 cggagtgttc cagaatgaca tagttcccac cagccattgc cactgcgcca aatttctcca
      301 tgactttctc cagctcctcc ttggacagtc ttgagccgag atacggcggg ttgtttggca
      361 caccgtcaac acaatcaaca accacaccat ctgcggaagc aataaccgcc ttgccccagc
      421 aaccatagtc ttcgttcttg ccagatggag ggtacatcat caggttaggt ttctggatca
      481 tcaaatcaaa agcaaattcc tggctgctgt tgaccctgtg gccatagttg gcattgtatg
      541 tattgatggc catccatata cctttggcag ggaaaatata cttgtttttc aaaacatatt
      601 ctgcaacttg caagcccagg tctctggaga aggtcttgtt acccttctgg aattcaatat
      661 ggatgttgca ttttgttgcc ggattgtccg agtaaaacct gaagggaatg tttataattg
      721 cggcctcctg gccgggctct attacaacag atttggacgt ctttttcaga tcaacaaagc
      781 ccgatgttcc aagaaaaatg ccctcctgga aatcatatgt catcttctca aatttctcta
      841 catatttccc aaaaagtgag ttcagttctt cacctttata aatcaaactg aagatttctc
      901 gctcatttgc aaaaatcgta aaagtcattg attttacttc aatgacttct ttgccatcgt
      961 ttgcaatttt cactgctttc aaaaggaaat cagattcaat accaagcttt acgtctcttg
     1021 ttgcattgta aataacatct gcaggaagtg ttgtaataaa aagattttca ccagaaccag
     1081 cattttcgtt tgtcatgcca caccccaatg aacaaatggc cagcaaaata gtcaacgcaa
     1141 aaggcatcag tttcatttcg acctcctgtc tacaaattat agcgacaggg tgggcccacg
     1201 tcaaaattgg cacatgcctg acgaaaccga gagacggggt ttcgtcaggc actggtatgg
     1261 aaactatttg atatggataa ctctggactg tcttgctgat ctaccaaagg aatcaacgca
     1321 ggttatcagg acctcaaaat caccctgact tgaataggaa ttctcaaaac tgaccagatt
     1381 gtctttgctc tgtatcgttt tggtggattt ggttccattg ccccaattca ctgtgatttc
     1441 atatggtggg gccccaccct cgacggagag gtcaagtttt atttcttcat ttactcttgc
     1501 ttcgtttgtc tttgtattaa tacccttcca agttgcgtca agaacaatct ctttcgtcgg
     1561 tggctgtttg caaacactta tcgtggcata atctgtgact gcttctgcgg aatcggacac
     1621 agcgctggcc ttgttggtta tcgtcagaca atttccggcg aaagacaagg attttgcaag
     1681 actgaatttc agcaaatagg tctttgactg acccggtgac aaactcccaa cctggtacct
     1741 gattttggaa gggccagcag cgccggacgg gagtgaagaa acatatttga gctcccttgg
     1801 gaaaacatca tcgagaacaa cgtttgtggc atcaagattg ccgatatttc tgatggttat
     1861 ctggaacgca aatatgtcat caatgttgta ctggaaagct tttgctttct ttgtgacttc
     1921 cagaatagct ttttttacag gtttttttgt gttgactgca acattgtttg acaacgccgg
     1981 cactccctgg tccagtgcgt gccttgaaaa agcctggttg tttatctgtg aatctaccgg
     2041 tgtttcttca actattgcag aaaaaccgaa cgaacgcggt gccgtgggtg
//
LOCUS       DAOSRI010000282         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_48642_length_2090_cov_3.826536, whole genome shotgun sequence.
ACCESSION   DAOSRI010000282 DAOSRI010000000
VERSION     DAOSRI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48642_length_2090_cov_3.826536"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK717_07500"
     CDS             complement(<1..554)
                     /locus_tag="PK717_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOR47540.1"
                     /translation="MKKFLAFSLLFVLLATGSTIREKSYYFESDEPHGEVLLHVEGGK
                     YYCLDKTLPQDAETIKTIYEQTELGKFHSYEMVFDELNDLSEKYPSKCHTVSLGQTIE
                     GRDIMALRFQPEKPSPAFLFMGCHHAREWMSVEVPLRLAHILAENPGNDKRIDKYLAN
                     YDIWVIPMVNPDGHSYSVKENRLW"
     gene            complement(551..1942)
                     /locus_tag="PK717_07505"
     CDS             complement(551..1942)
                     /locus_tag="PK717_07505"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOR47541.1"
                     /translation="MGKSKKKKARARLIRAFVRLVLLLGAAFLVLWVLWTGFGCLFRS
                     GGGNNNTSLYFYDDILGTLTPIERKISSQPDLATNAVLELIKGPQPGEHVRATLNPRT
                     KLIWLKTEGGVATVNLSKEIQTRISSDVPEKMAIYSIINTLTSIKGINAVEFLIDGTK
                     PTFFQSSFDASGQFTQLLEENKNTRDCYLYFPDLESKYIAVETAKVRNVLDPATFAGL
                     VAKRLIQGPQSPYLTNVFPPSTKVLSVKLAEGMLTINLSIEARNQNTGADGELAMMQS
                     LVWTLTEIPGVRKVKVLVAGKTADTLGGHISTFDPIERMDQVLFDENVKEGGKAVLVF
                     LSVDMGDGIYLPVPRIRSIRIDADPIQQVVNLLLQGTNADERDFSIGTCIPGETKLNS
                     IKKEGDHYLVDLNLDTKAIQDASYEATMVRQLVMTITELDKNSAVLLSINGEIRESLP
                     YGTKTGTPILRGP"
BASE COUNT          493 a          478 c          474 g          645 t
ORIGIN      
        1 ctccaaagcc tgttttcttt gactgaatag gagtggccgt cgggatttac catggggatg
       61 acccagatgt catagttcgc cagatatttg tcaattcttt tgtcattgcc tggattttcg
      121 gccaaaatgt gggcaagcct gagaggaacc tcaacactca tccattccct tgcatggtgg
      181 caacccataa aaagaaatgc tggagagggt ttttctggct ggaatctcaa ggccatgata
      241 tcgcggccct cgattgtttg cccaagcgac accgtgtgac attttgaagg atatttttcc
      301 gacaggtcat ttagctcatc aaagaccatt tcatatgaat gaaatttgcc cagttctgtt
      361 tgttcgtaga tggttttgat ggtttcggca tcttgtggga gtgtcttgtc gaggcagtaa
      421 tattttcctc cctcaacgtg caggagcact tcaccatgcg gttcatcaga ctcgaaatag
      481 tatgattttt cccttattgt cgagcctgtt gccagcaaga caaagagtag tgaaaatgct
      541 aggaattttt tcaagggccc ctcaagattg gggtaccggt cttggtgccg tatggcaagc
      601 tttccctgat ttcaccattg atggacaaaa gaacggcaga attcttgtct agttctgtga
      661 ttgtcatcac aagctgtctg accattgttg cctcgtaact ggcgtcctga attgcttttg
      721 tgtcgagatt cagatcaaca agataatgat cgccttcttt ttttatggag ttgagttttg
      781 tttcacccgg gatgcaagtg ccaatcgaga agtccctctc gtcggcattt gttccctgta
      841 acaataaatt tacaacctgc tggattggat cggcatcaat ccttatgctc cttatccttg
      901 gcaccggcag gtaaatgccg tcacccatgt ccaccgacag gaacacaaga acggccttgc
      961 cgccttcttt tacattctcg tcgaaaagca cctggtccat tctttcgatt gggtcaaatg
     1021 ttgaaatgtg accgccaagc gtgtctgcgg ttttgcctgc aacaaggacc ttaacttttc
     1081 ttacacccgg aatctctgtc aacgtccaga caagagactg catcattgca agctctccat
     1141 ctgcccccgt gttctggttt cttgcttcaa tcgacaggtt gatggtgagc attccttcgg
     1201 ctagttttac cgaaaggact ttcgtggatg gtgggaaaac gtttgtcaag tacggtgatt
     1261 gtggcccctg aatgagtctt tttgcaacaa gcccggcaaa tgtggcagga tcaagaacgt
     1321 ttctgacctt tgccgtttcg actgctatgt attttgattc caggtccggg aaatacagat
     1381 agcagtccct ggtgtttttg ttttcctcca ggagttgtgt aaattgccct gaggcatcaa
     1441 aagagctctg gaaaaatgtt ggttttgttc catcaatcaa gaattctact gcgtttatac
     1501 cttttatgga ggtcaaagta ttgatgatgg agtagattgc catcttttcc ggaacatcgg
     1561 aagatattcg ggtttgtatc tctttgctca ggttgactgt cgcaactccg ccctcggttt
     1621 ttaaccagat aagttttgtt ctgggattta acgtcgctct gacgtgttcg cctggctgcg
     1681 gcccttttat tagttccaga acggcatttg ttgccaaatc tggctgggag cttatcttgc
     1741 gttcaatagg ggtgagtgtg ccaagtatat catcataaaa atacaaggaa gtgttattgt
     1801 tcccgcctcc ggacctgaac aaacacccaa accctgtcca gaggacccac agaacaagaa
     1861 atgctgcacc caaaaggagc acaagcctga caaatgccct tatcagtctt gccctggcct
     1921 tctttttctt tgatttgccc attgcttggt attttacgct ttgctcctta aggttgcaac
     1981 gctttcgatt ggattatatc ataaagacat gataaacctg atactccaca atccgaccaa
     2041 gataatattt ggtgttggtg tattgtctca acttgctact gaagcttcaa
//
LOCUS       DAOSRI010000283         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_48644_length_2090_cov_3.626044, whole genome shotgun sequence.
ACCESSION   DAOSRI010000283 DAOSRI010000000
VERSION     DAOSRI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48644_length_2090_cov_3.626044"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..>2090
                     /locus_tag="PK717_07510"
     CDS             183..>2090
                     /locus_tag="PK717_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR47542.1"
                     /translation="MKNKRFLKYFKDSLLIMLSALFVIGSFGGAKAQEKFLRIEPGEL
                     VINLGENAKLNAIATDENGNKVSQPDIEWSVEGDAVSIEKTSGTQIMVNAKDYGFSTI
                     TARWGNTTATSRVMVYMAQEIQLEKNLKLTPSGEVTIKVGQKLDISCKISDENGKDVP
                     YARVQWSSACSGTTKETGANRLGFSSDKPTVCQIRASYMGLEATLTVKVVEMCLKITP
                     NPVILGWLESYGKSEITLANCSPLPLAVNLSEQAAWASLSETKFTIPANGTKKIYVSS
                     TKPTPAPGGVYNFDVTAAWENKKTPVAVIGYTRPLEQAKPKQTFTQGWINNGSNHNRS
                     YAVPEGNGTETNALSVQWAQNLGLSGNMMMLDKKLYLVSANYDFVGVNCYNLETGGRI
                     WTSTNVRDKLGFPLDPKTNAMIAEGKEFFCIGGSLFAVSLADGKLLWSWQPNGSEWVG
                     LFMTYANGLVWTGGSSLHCFDPKTGLKLGSYGENTTMYPAYMDGKIYFVSVSSSLFGT
                     EYSLEMLDPATEKITTKYRWDENATGASGGKIDGIYCSEGYICIATYKNIKIFNTKSK
                     NGFDPTILYDFTTSLEDRINVSIIGGKAYFHDDNELYCFDLSSATYLWRVKSSDPFVG
                     LFSISGSRIFIS"
BASE COUNT          653 a          460 c          485 g          492 t
ORIGIN      
        1 agaaccaaga cgatcaccag gaagataact gtaaaataag ctcaactttt tgactaactg
       61 gagcgtttga agccccgagg ccattaagcc tcggggcttt tgttttaagt tcactctttg
      121 caagtattaa aaaacataag aacaaaacag aggtttttcg tgtattctat atttggaaga
      181 aaatgaaaaa caaaagattt ttgaaatatt ttaaggattc attactgata atgctttcag
      241 ctctgtttgt gattgggagt tttggtggtg caaaggcgca agaaaaattc ctgagaatcg
      301 agccaggtga gctggtcatc aatcttggcg agaatgcaaa actgaacgcc atcgcaaccg
      361 atgaaaatgg aaacaaagtt tcacagcctg atattgaatg gtctgtcgaa ggcgatgccg
      421 tttcaatcga aaaaacgtcc ggcacccaaa tcatggtaaa cgccaaagat tacggtttct
      481 cgacaataac ggctagatgg ggtaacacca ctgccacctc aagggtcatg gtctacatgg
      541 cccaggagat tcagcttgaa aaaaacctga aactcacccc atcaggcgag gtgaccataa
      601 aggtgggcca aaagctagac atatcatgca aaattagcga cgagaacggg aaggatgtcc
      661 catacgcaag ggtccagtgg agcagcgcct gcagcgggac aaccaaggaa actggggcca
      721 acagattggg attctcaagc gacaaaccga cagtgtgcca gataagggca agctacatgg
      781 gactggaggc gacactcacc gtcaaggttg tcgaaatgtg cctgaagatc acgccaaacc
      841 cggtaatcct tggctggctg gaatcctatg ggaaatctga aataacactt gccaactgct
      901 cgccgctccc gcttgccgta aacctctccg agcaagcggc gtgggcatcg ctctcggaaa
      961 caaagttcac cataccggca aatggcacaa aaaagatata cgtttcttca acaaagccaa
     1021 cacctgcccc tggaggggtc tataacttcg acgtcacagc cgcatgggaa aacaaaaaga
     1081 caccggttgc tgttatcggc tacacaagac cgcttgaaca ggcaaaacca aaacagacat
     1141 tcactcaggg ctggataaac aatggttcaa accacaacag gagttatgcc gtgccagaag
     1201 gaaacggcac agaaacaaat gccctttctg tccagtgggc gcagaacctc ggcttgagcg
     1261 gcaacatgat gatgctggac aagaagctct accttgtttc ggcaaactac gactttgtcg
     1321 gcgtaaactg ctacaatctc gaaactggag gcaggatttg gacctccact aatgtgcgcg
     1381 acaaactggg ttttccactg gatccaaaaa ccaacgctat gattgcggaa ggcaaggaat
     1441 ttttctgcat tggaggatct ctgtttgctg tttcgcttgc agatgggaaa ctcttgtggt
     1501 catggcaacc aaacggatct gaatgggtag gtctatttat gacctatgcc aacgggctcg
     1561 tctggactgg cggcagtagt ctccactgct tcgacccaaa aaccggcctg aagctgggtt
     1621 cttatggcga gaatacaaca atgtatccag cctacatgga cggaaaaatt tactttgttt
     1681 ctgtaagtag cagtttattt ggtactgaat actccctcga aatgcttgat ccagcaacag
     1741 aaaaaataac aacaaaatac agatgggatg aaaatgcaac tggtgcaagt ggcggaaaaa
     1801 tagatggaat ttattgtagt gaaggatata tctgtatagc tacctataaa aatattaaaa
     1861 tattcaacac aaaaagcaaa aatggttttg atccaaccat tctttatgat tttaccacgt
     1921 ctctcgaaga tagaatcaat gtttctatca ttggaggcaa ggcatatttt cacgatgaca
     1981 acgaacttta ctgtttcgat ctgtcaagtg caacctatct ctggagagta aagtcgtctg
     2041 acccatttgt agggcttttt tcaataagcg gcagcaggat ttttatctcc
//
LOCUS       DAOSRI010000284         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_49057_length_2078_cov_4.744439, whole genome shotgun sequence.
ACCESSION   DAOSRI010000284 DAOSRI010000000
VERSION     DAOSRI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49057_length_2078_cov_4.744439"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PK717_07515"
     CDS             <1..1247
                     /locus_tag="PK717_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOR47543.1"
                     /translation="IARAMGRKFVRVSLGGIKDEAEIRGHRRTYVGALPGRIIQGIKL
                     AGSSNPVFLLDEIDKVGLDFRGDPTAALLEALDPEQNTAFSDHYLEIPYDLSGVLFVT
                     TANVSHTIPRALFDRMELITIPGYTQYDKIHIAKKFLIPKQLKENGLTADDVELPDET
                     IDGVIRLYTREAGLRTLERRIASIMRKVAKNKVETGKLEKTTILPENLSDYLDVPIFK
                     SDVQLKKDEVGIATGLAVTENGGEVLFIECTLMEGSGKLILTGQLGDVMKESATAAMS
                     FVRSHRKELGLPESYSADKTDIHIHFPEGAIPKDGPSAGIGIITSLVSAISGLPVRSD
                     VAMTGEITLRGSVLPIGGVKEKVLAAHRYGIKTVILPKDNEADSKKIPADVLSEIKIV
                     FVENVFDVIKIAIKHQTPRRKK"
     gene            1244..>2078
                     /locus_tag="PK717_07520"
     CDS             1244..>2078
                     /locus_tag="PK717_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR47544.1"
                     /translation="MTRVGLVSLGCPKALVDSEQMMASLAKEGYEIVLGDQDCDILFI
                     NTCSFIGDAREESEQTIKNAIKLKRAGKIKRIVVTGCLPQLVGEQIKEKYKGIDAFLG
                     TGKNDLAALAANGSLAFERSAVHAPQGSPLPRLQLTLPHVAYLRIAEGCSHKCSFCTI
                     PSIRGPYHSFELKDLVLEARALASIGVKEIIIIAQDTSIVGLDAKDPYDLKKLMEELS
                     QIGGIEWIRVMYLNPMHLTENVLAAFTVKKVLPYFDIPFQHINDKILSAMGRLSPGKD
                     DI"
BASE COUNT          602 a          463 c          503 g          510 t
ORIGIN      
        1 caattgccag ggcaatgggc aggaaatttg taagagtttc tcttggtgga ataaaggatg
       61 aggccgagat aagaggacac aggcgcactt atgttggtgc cttgccaggc agaataatcc
      121 aggggataaa gcttgcaggt tcctcaaacc cagttttttt gctggacgaa attgacaagg
      181 tagggctgga tttcagggga gatcctacag cggcgttact tgaggcactt gaccctgaac
      241 aaaatactgc attttccgac cattacctgg aaattcccta tgatttgtca ggcgtgcttt
      301 ttgtgacaac tgccaatgtt tcccacacca ttcccagagc tcttttcgac aggatggagc
      361 tcatcacaat ccctggttac acccagtatg acaaaatcca tattgcaaag aagttcctca
      421 taccaaagca gctaaaggaa aatggcctta ctgcagatga tgtagaattg ccggatgaaa
      481 caatagacgg tgtgataaga ctttacacca gggaagctgg cctcaggact ctcgaacgca
      541 ggattgcttc gatcatgcgc aaggtggcta aaaacaaggt tgaaactggt aaattggaga
      601 aaacaacaat actcccagag aatctttcag attatcttga tgttccgata ttcaagtccg
      661 atgtccagct caagaaggac gaagttggaa ttgccacggg actagccgtt acagaaaatg
      721 gcggtgaagt cctcttcatc gaatgcacac tgatggaggg ctcaggaaag ctgatactca
      781 ctggccagct tggcgatgta atgaaagaat ctgcaacagc cgccatgtca tttgtgagga
      841 gccacaggaa agagcttggc cttccagaat cttacagcgc tgacaaaacc gatatacaca
      901 tacatttccc tgaaggtgct atccccaagg atggcccgtc cgcaggaatc ggaataataa
      961 cctcgcttgt ttctgcaatt tcaggtttgc ctgtcagatc agatgttgca atgaccggag
     1021 agataaccct gagaggttct gttcttccaa ttggtggagt gaaagaaaaa gtcctggccg
     1081 cacaccgtta tggtattaaa actgtcatct tgccaaaaga caatgaagct gacagtaaaa
     1141 aaataccggc ggatgtgctc tccgagataa aaatagtttt tgtggaaaat gtttttgacg
     1201 tcatcaaaat tgccataaag caccaaaccc cgcggaggaa gaagtgacga gggtcgggct
     1261 tgtcagtctt ggttgcccaa aggccctggt ggattccgaa cagatgatgg cctctcttgc
     1321 aaaagagggc tatgagatag tccttggcga tcaggattgc gacattcttt tcataaatac
     1381 atgctctttt attggagacg caagggaaga atctgaacaa acgatcaaaa atgctatcaa
     1441 gctcaaaagg gccggcaaaa taaagcgtat tgttgtgact ggctgtcttc cccagcttgt
     1501 tggggaacag ataaaggaaa aatataaggg aattgatgct tttctcggca ctggcaagaa
     1561 cgaccttgct gcactggcgg ccaatggatc acttgcattt gaaagatctg ctgtccatgc
     1621 gccacagggc tcaccgctcc caagactcca gctgacactg ccccatgtgg catatctaag
     1681 gattgccgag gggtgctccc ataaatgctc tttttgcact attccttcaa taaggggccc
     1741 ctaccacagt tttgaactta aagacctcgt tcttgaggca agggcgcttg cttcaattgg
     1801 cgtcaaggaa atcatcatca ttgcacaaga cacctccatt gtcgggcttg acgccaaaga
     1861 cccttatgat ttgaagaaat tgatggagga gctctcgcaa attggtggca ttgagtggat
     1921 aagggtgatg tacctgaacc cgatgcacct tacggaaaat gtccttgcgg cctttactgt
     1981 taaaaaagtg cttccatatt ttgatatccc attccagcac ataaacgaca agattctttc
     2041 ggcaatggga agactcagtc caggaaaaga cgacattc
//
LOCUS       DAOSRI010000285         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_49122_length_2076_cov_3.655616, whole genome shotgun sequence.
ACCESSION   DAOSRI010000285 DAOSRI010000000
VERSION     DAOSRI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49122_length_2076_cov_3.655616"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PK717_07525"
     CDS             complement(<1..1004)
                     /locus_tag="PK717_07525"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOR47545.1"
                     /translation="MKKFLSLTLCFAILMGLVPGIFVPGKASAISDFGVVPIGGPPKL
                     GTFVSPSWLASEGNRVYVVEQAAFRTQAIMKNGQSQFAFGLHGNHEEQISDPGGIFAY
                     REKIYWIDRSQGKILVRGNKVDSPYEGEFTPFGTDGRKLLKRPEGIWIDQNRIYIANS
                     GASNVVVLDLNGKFINTIGSKGIEDGKLSSPAGVCVVASKLFVTDPQNGRIVIYDLDG
                     KFIQNISSSHPFGITALESKIFVTDAVEGKITILNADGATVSSFGSPGSGQGQLGNPI
                     GITILDGKIYVASNTNNRVDVFEQDGKYAGILGLPVEQAASSFGRPAGVAYNGENIAA
                     A"
     gene            complement(1693..>2076)
                     /locus_tag="PK717_07530"
     CDS             complement(1693..>2076)
                     /locus_tag="PK717_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47546.1"
                     /translation="EKVMEILEILKDNVDAKSKYEIESLDAIMELLESKKDLIEDKSE
                     ELLEGIEEIRDQFDDDDDSGKSVEGLGNWATEISQTSKKILLENVMDESLDKAISIMR
                     ENTRKLYISFEETAQALMDLVHQQD"
BASE COUNT          546 a          440 c          407 g          683 t
ORIGIN      
        1 gcagccgcga tgttttcgcc attataggcc acgcctgctg gtctgccgaa ggaggaggca
       61 gcctgttcga ctggaaggcc caaaatgcct gcgtattttc catcttgctc aaagacatct
      121 acgcggttgt ttgtgtttga tgcaacataa atcttgccat caagaattgt gattccgatt
      181 gggttgccaa gctgtccttg tcctgatcct ggggagccga atgaggaaac cgtggcacca
      241 tcggcattaa ggattgttat tttaccttct acggcatcgg ttacaaagat cttgctttcg
      301 agggccgtta ttccaaatgg gtgggaagaa gatatattct gaatgaattt tccgtcaaga
      361 tcataaatca ctatcctgcc attttgtgga tcggttacaa acaatttgga tgctacaacg
      421 caaacaccgg ctgggctgga aagcttacca tcttcaattc ccttggaacc gattgtgttg
      481 atgaatttgc cgttaaggtc cagtacaaca acatttgatg cgccggagtt ggctatatag
      541 atcctgtttt ggtctatcca gatgccctcc ggtctcttaa ggagcttcct gccatcggta
      601 ccaaatggcg tgaattcgcc ttcgtatggt gaatctacct tgttgcccct tacgagaatt
      661 ttgccctgcg acctgtcaat ccagtatatc ttttctctat aggcaaatat tccacctgga
      721 tcgcttatct gttcctcatg gtttccatga agaccaaaag cgaactggga ttgcccattc
      781 ttcattatgg cttgtgttct gaatgctgcc tgctcgacga cgtaaactct gttgccttct
      841 gatgctagcc aggaggggga cacgaaggta ccaagttttg gtggtccacc aattggaaca
      901 acaccaaaat cagagatggc acttgctttt cctggtacaa atatacctgg cactagcccc
      961 atcaggatcg caaaacaaag tgttaatgat aaaaatttct tcattaaagc ccccttctag
     1021 gttgttgtaa atgtataggt ccggtcattg tgaaggaagt atattgtcta caaccttaaa
     1081 ctcaacgata tttggtttgt gataagctta aagttaagtt tgtacccatt ttgattaaag
     1141 cttcacgaat tattacagat cgttccagca aattttaaaa aatagggttg atttctactg
     1201 tgttcttttg cgtaaactgt tttttgatca ttaaaagata aatttattaa gatgcaaaag
     1261 taaaaaacat attttaactg agggcttctt tgcatttttt gctaagtgga accaaaattt
     1321 gttgacaggg tggctcaagg gtttaagatg gactcgccca atttgggcca ccctcctttt
     1381 ctttccgggg acttgtcccc aaaaccctaa cccccttccg gcgggggttt cggtttttta
     1441 ttataggaaa tcaccttgcc atataaatgg atcaattgtg aataataatg tatgactatt
     1501 tatagtccaa tcaaaaaata caagctatat ttggtttata tatctgattg gatttggata
     1561 aattgtgaaa ccagaaaaaa tttagctaga ccattaaaac aaaattagcg tctagaaatt
     1621 tataatggaa tgctatttta taagccattt tttcaaaatc attccaatct gacaaatgct
     1681 tatttccaaa tattaatcct gctggtgcac cagatccatg agtgcttgtg ctgtttcttc
     1741 aaaagatata tagagttttc ttgtgttttc cctcatgata ctgattgcct tgtccagtga
     1801 ttcatccatg acgttttcca gcaggatttt tttggatgtt tgcgagattt cggtggccca
     1861 attacccaat ccttctacag actttccact gtcatcatcg tcatcgaact ggtcccttat
     1921 ttcttcaatc ccttccagga gttcttctga tttgtcttca atgaggtctt ttttgctttc
     1981 cagcaattcc ataattgcat ccagcgattc aatctcatat ttggactttg catctacatt
     2041 gtctttaagt atttcaagaa tctccatcac cttttc
//
LOCUS       DAOSRI010000286         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_49756_length_2058_cov_3.481777, whole genome shotgun sequence.
ACCESSION   DAOSRI010000286 DAOSRI010000000
VERSION     DAOSRI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49756_length_2058_cov_3.481777"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK717_07535"
     CDS             complement(<1..539)
                     /locus_tag="PK717_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47547.1"
                     /translation="MPERKPEWQVIIDLLRATDSRELNRISRKLLNKLVRMEVKGAID
                     LLERFDQAYMNTRGDENQPSQKTNLGEIELIFEETFKIAKTCLSEDELSSMIMKWVSE
                     GRTMFLTLATEKQGISLLEITENLNRFCMLPENERLLSEAEENGIRVSLVRRFLTDSP
                     QFINVAKKYIRIGDISKMI"
     gene            complement(670..>2058)
                     /locus_tag="PK717_07540"
     CDS             complement(670..>2058)
                     /locus_tag="PK717_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47548.1"
                     /translation="VGTIIIMIVLSPILFLITAVVLVLMIFITMKIGVKSQAHFKNQQ
                     IILGKLNGNIEETISGLREIKVFNHEGAAKQSFYKLNEQFYHTASKANFLAGIIMPIM
                     VNLNNVCYAATAVFGGILTLMNMFDIGSLAAFLQYSRQVGQPITMITSQINNVLAALA
                     GAERIFEVMDQKPEVDNGHVTLVNAHRTIDGAIEECQERTGLWAWKYPHENGTSTYTE
                     LKGDVRFFDVSFSYEPNVPVLKDISLYAKPGQKIAFVGSTGAGKTTITNLINRFYDVP
                     EGKIHYDGINITEIKKADLRRSLGMVLQDTHLFTGTVMDNIRYGRLDATDEECIKAAE
                     IASADSFITRLPQGYHTMIEGDGANLSQGQRQLIAIARANVASPPVMILDEATSSIDT
                     RTERLIEKGLDALMENRTVFVIAHRLSTVRNSKAIMVLEHGEIIERGNHEELLALKGR
                     YYQLYTGQNQLE"
BASE COUNT          470 a          455 c          477 g          656 t
ORIGIN      
        1 tggatcatct tggaaatgtc gccaattctt atgtactttt ttgcgacatt gatgaattgc
       61 ggactgtctg tgaggaagcg cctgaccagc gaaaccctta taccattttc ctcggcttct
      121 gagagtagtc tttcattctc tggcaacatg cagaagcggt tgagattttc ggttatttcc
      181 aagagcgaga tgccctgttt ttcagtcgcg agggtcaaaa acattgttct gccctctgac
      241 acccacttca ttatcattga cgacaattca tcttcgctaa ggcatgtttt ggctatcttg
      301 aatgtttctt caaaaataag ttctatttcg ccaagattcg ttttttgtga tggctggttt
      361 tcatcacccc ttgtgttcat atatgcctga tcaaaacgct caagaaggtc gatggctcct
      421 ttgacctcca tcctcaccag cttgttgagg agtttcctgg agatgcggtt caattccctt
      481 gagtctgtgg ccctcaatag gtcgataatt acctgccact ctggttttct ttccggcatg
      541 gagacatttt agcttttttg tctttttttg gcaatgattt gagaaatgct ggatattaat
      601 tatttttgct gaaagacctg gaagaaattg ctgtgggccg ctaagcccac agcaattcta
      661 agacagaacc tattcgagct ggttctgccc agtgtagagt tggtagtatc ttccttttaa
      721 ggcaaggagt tcctcgtggt tgcctctttc aataatttcc ccgtgctcca ggaccataat
      781 ggctttggag ttccggactg ttgagagcct gtgggcgatc acaaagactg tcctgttttc
      841 catcagagcg tccagtcctt tttcgatcag tctttcggtg cgggtatcga ttgagctggt
      901 tgcctcatca aggatcatga ctggagggga tgccacattg gcacgggcta tggcgataag
      961 ctgcctttgc ccctgcgaga gattggcccc gtctccttca atcatggtgt ggtacccttg
     1021 tggcaagcgg gtgatgaaag agtctgcgct tgcaatttct gcggccttaa tgcattcctc
     1081 gtctgttgcg tcgagtcgtc catagcggat attgtccatg actgtgccgg taaaaagatg
     1141 tgtatcctgt aataccatcc cgagggagcg gcgcaggtca gcctttttga tttcagttat
     1201 gttgatccca tcataatgga tttttccttc aggcacatca taaaagcggt tgatgaggtt
     1261 ggtaatggtg gtttttccag cacctgtcga accaacaaat gcaatctttt ggccaggttt
     1321 tgcgtaaaga cttatgtctt tgagtaccgg tacattgggc tcataactaa aacttacatc
     1381 aaagaatcgc acatctcctt tcaattcggt ataagttgac gtgccgtttt cgtgtggata
     1441 tttccatgcc cagagaccag tgcgttcctg gcattcttca atggccccat ctattgtacg
     1501 atgagcattg accagggtca catgaccatt gtcaacttct ggtttctgat ccataacttc
     1561 gaagattcgc tctgcgcctg cgagggccgc tagaacattg ttgatttggc ttgtgatcat
     1621 ggtgattggc tgaccaacct ggcgggaata ttgcaagaag gcggcgagag agccgatatc
     1681 aaacatgttc atgagtgtca gtattccgcc aaaaactgct gtcgcagcgt agcagacatt
     1741 gttcaagttc accattattg gcatgatgat gccggcaaga aaattcgctt ttgatgctgt
     1801 atggtagaac tgttcgttta gtttgtaaaa cgattgtttg gcggcccctt catgattgaa
     1861 tacctttatt tctctcagtc cgctaatggt ctcttcaatg tttccattga gtttgcctag
     1921 tatgatctgc tggtttttga agtgtgcctg gcttttaaca cctattttca tcgtgataaa
     1981 aatcatcaat acaagtacga cagccgtgat caaaaacaaa attggactga gtacaatcat
     2041 tattatgatt gtgccaac
//
LOCUS       DAOSRI010000287         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_49884_length_2054_cov_4.005003, whole genome shotgun sequence.
ACCESSION   DAOSRI010000287 DAOSRI010000000
VERSION     DAOSRI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49884_length_2054_cov_4.005003"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK717_07545"
     CDS             complement(<1..662)
                     /locus_tag="PK717_07545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOR47549.1"
                     /translation="MQQRFKIASELALDVGTLLLDMRFCDLKTRTKSSTCDLVTEADY
                     RSQTLITNTIESNFPEDSILSEEAFSKQGTSEWQWIIDPLDGTTNYAHGMPVYCVSIA
                     AYKDGEPMIGVIYDPNRRELFEAKRGEGACLNDKSIYVSKRSNLQVSMLATGFPYDKA
                     RDLKDNNVNHFIAFLLNTHAVRRLGSAALDLCYVAAGRLDGYWELKLKPWDMAAGVLM
                     VM"
     gene            complement(634..1095)
                     /locus_tag="PK717_07550"
     CDS             complement(634..1095)
                     /locus_tag="PK717_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR47550.1"
                     /translation="MVPMTHEESAGGVVFLEGKVVILQRKSNKNWILPKGHLEEGETH
                     EQAAIREVFEETHLQADPITPIGDTHYQFKIDESNVIDKSVKYFLMLAKSRKVKTEAF
                     FSYYLVVPPAKAIRLLTFPQDKKILRSAWFEYRRLAKEGAIPDATKVQNRF"
     gene            complement(1115..>2054)
                     /locus_tag="PK717_07555"
     CDS             complement(1115..>2054)
                     /locus_tag="PK717_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR47551.1"
                     /translation="LQDTSPKIIDLPFKSQVVFQMEISARVKGYMFVASKVGSTFNTD
                     IADMMKSLSNQLALAIQKNLIGDELKDKVMMLEEMNKGKDEFIASMTHELRTPLTTLL
                     GFLELLDQRKPNMTKEQIEFISIASQSAESLSWLIEDLIDLGRLKNGKFTFHNNRNFF
                     SDIFYQETGKLAKSAWQKGITVENMFVGENRSLLLDKKVVSRIIGNLCKASLIYLHKG
                     DKMRFYCKVVDRNLVFSVIDDGKQWDKEDLDYISDVFTRIPSKTLEEHPGSVGLALSY
                     AKEFTRAMGGTFEVRTLPFGNYIKIIIPEKPNRELE"
BASE COUNT          488 a          450 c          428 g          688 t
ORIGIN      
        1 tccatgacca tgaggacccc tgccgccatg tcccatggtt tcagtttcag ttcccagtag
       61 ccgtccagcc ttccagcggc gacataacat agatcaagtg ccgctgaacc gagccttctg
      121 acggcatgcg tattgagcaa aaatgcaatg aaatggttta cattgttgtc ttttaggtct
      181 cttgccttgt catatgggaa tcctgttgca agcatggaaa cctggagatt gcttcttttt
      241 gaaacgtaga tgcttttgtc attgagacat gcgccttcgc cccttttcgc ttcaaacagc
      301 tctctcctgt tggggtcgta tatgaccccg atcatgggtt cgccatcctt gtatgccgca
      361 attgaaacac agtaaacagg cattccatgt gcgtaatttg ttgttccatc cagtgggtca
      421 attatccatt gccattccga tgtcccctgt ttgctgaatg cctcttctga caggatggag
      481 tcctcaggaa agtttgactc tatggtattt gttatgagag tttgtgatct gtagtcggcc
      541 tctgttacaa ggtcgcatgt ggaggatttg gtgcgtgttt tcagatcaca gaatctcatg
      601 tccaggagta gtgtgcctac gtcaagtgct agttcagaag cgattttgaa cctttgttgc
      661 atcaggtatg gcaccttctt ttgctaagcg cctgtattcg aaccaggccg agcgaagaat
      721 ttttttgtcc tgcgggaacg tcaagagcct tattgctttt gctggcggaa caaccaaata
      781 atatgagaag aatgcctctg tttttacttt tcttgatttt gcaagcatca gaaaatactt
      841 gactgatttg tcaattacat tgctttcatc aatcttgaat tgatagtggg tgtcgccaat
      901 gggtgttatt ggatcggctt gcaaatgggt ttcttcgaac acttccctta ttgcagcttg
      961 ctcgtgtgtt tcaccttcct caaggtgacc cttcggtaat atccaatttt tattgctttt
     1021 tctttgcagg atgacgactt tcccttccag gaacacaact cctcctgccg attcttcatg
     1081 agtcatggga accatattat ccaattagga ggagttattc aagctcccgg tttggttttt
     1141 ccggaatgat tatttttatg tagtttccaa aaggcaatgt ccttacttca aatgttcccc
     1201 ccattgctct ggtgaattcc tttgcatagg agagtgccaa gccaactgaa ccggggtgtt
     1261 cctcgagtgt ttttgaaggg atccttgtga aaacgtctga aatataatcc aggtcttcct
     1321 tgtcccattg ctttccatca tcaatcactg aaaatacaag gtttctgtca actaccttgc
     1381 aataaaacct cattttatca cctttatgaa gatatatgag tgatgctttg caaagattgc
     1441 caattattct ggaaacaact tttttgtcaa gcaggaggga cctgttctca ccaacaaaca
     1501 tgttctccac agttattccc ttttgccagg cgctctttgc cagttttcct gtctcctggt
     1561 aaaatatgtc agagaagaaa tttctattgt tatggaaagt gaattttcca ttcttcagcc
     1621 ttccaagatc aatcaggtct tcaatcagcc atgagagtga ttctgcagac tgcgaagcta
     1681 tacttatgaa ttcgatttgc tcctttgtca tatttggttt tctttggtca aggagttcaa
     1741 gaaaaccaag gagtgttgta agaggtgtcc tcaattcatg tgtcattgag gcgatgaatt
     1801 catctttgcc cttgttcatt tcttcaagca tcatgacctt gtctttcaac tcatcaccaa
     1861 tcaagttttt ctggattgcc agcgcgagct ggtttgaaag acttttcatc atgtctgcaa
     1921 tatctgtatt gaatgtgcta ccaacttttg atgcaacaaa catgtatcct tttactcttg
     1981 ctgaaatttc catctggaaa acaacctggg atttgaatgg caggtcgatt atttttggag
     2041 aagtgtcttg caat
//
LOCUS       DAOSRI010000288         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50042_length_2050_cov_2.999499, whole genome shotgun sequence.
ACCESSION   DAOSRI010000288 DAOSRI010000000
VERSION     DAOSRI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50042_length_2050_cov_2.999499"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK717_07560"
     CDS             complement(<1..302)
                     /locus_tag="PK717_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR47552.1"
                     /translation="MKNILIFKDKAVNHNREMENYDVIVVGAGHAGMEASLASCRIGA
                     KTLLLTINTDQMAYMPCNPAMGGPGKTQVLCELHALGGASAILSEESATSIRLLNRS"
     gene            316..399
                     /locus_tag="PK717_07565"
     tRNA            316..399
                     /locus_tag="PK717_07565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:tga)
     gene            409..948
                     /locus_tag="PK717_07570"
     CDS             409..948
                     /locus_tag="PK717_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47553.1"
                     /translation="MRKPLAILALFSLILAISCGGPIGTVKSAKDEYTISYFRGWKPV
                     TEFKQPNTDNLEVVFVREDLPSFIGVKVASQTRLDIQQGHIREVTAEYAKDAEKKVPG
                     YTLVSHEYKNVPNALGDERLELVFSAIEGGQSYTIIQHIYAIIGKVLYVTGTYPEGDE
                     ALANQIKDTMESFVVAPKK"
     gene            1022..1762
                     /locus_tag="PK717_07575"
     CDS             1022..1762
                     /locus_tag="PK717_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47554.1"
                     /translation="MRKLLIVALLLSISLTAVGCGCGGESGGNGNGGKIDYGDKNPNE
                     GKIISSTSTDPNALKPAKDKELVGEMKIPPHPVLIWTLYAFEGLEVAGEQYQGWTEVP
                     LTKEMYDSGTYKMFTWDKNPKVTFKVVASKDKAVTNSDPATIKPQLENYIASIQATKD
                     KDGEFKHTQSLIKGIWQSYSYKKDGADYKGKVLAYSLRGIRVSETEFGPGTFLKIIGE
                     GPSAAYDEAVKDTMDYMIQQVMITQVSE"
     gene            1816..>2050
                     /locus_tag="PK717_07580"
     CDS             1816..>2050
                     /locus_tag="PK717_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47555.1"
                     /translation="MATFFALFLISCSGQSVIKWGGESYYVSYDKNFFGVVSEANDEL
                     QLNILDVKDSPIIKIIAVPDFKLSEGVLVSDLLD"
BASE COUNT          597 a          451 c          493 g          509 t
ORIGIN      
        1 gaacgattga gcaaccttat ggatgttgcc gattcttcag aaagaatggc agaggcgcca
       61 ccaagggcat gcagctcgca taaaacctgg gttttacccg gtccacccat tgctgggtta
      121 catggcatgt atgccatctg gtcagtattt attgtcagaa gaagtgtttt ggcaccaatg
      181 cgacaggaag caagagaggc ttccattcct gcatgtccgg caccaaccac aataacatcg
      241 tagttttcca tttctctatt atgattgacc gccttgtcct tgaaaatcaa gatgtttttc
      301 ataaactcca ttcccggaag gatggccgag cggtctatgg cgctgcactt gaaatgcaga
      361 gggtgaaagc ccgcgggggt tcaaatcccc ctccttccgg aggaaaaatt gagaaaaccc
      421 cttgctattc tggcgctttt ttcgctaatc ttggccatat cttgtggtgg ccctattgga
      481 acagtcaaat cagccaagga cgaatataca atatcttact tcaggggctg gaaaccggta
      541 actgagttca aacagccaaa caccgacaac cttgaggtag tttttgtaag ggaagatctc
      601 ccttcgttta ttggcgtcaa ggtagccagc cagacgagac ttgatattca gcagggtcat
      661 atcagggaag tcactgcaga atatgcgaaa gacgctgaaa agaaagtccc gggctacacc
      721 cttgtttccc atgagtacaa aaatgtccct aatgcacttg gcgatgaaag acttgaactt
      781 gtcttctcgg caatagaggg cggacagtca tatacaatta ttcaacatat ttatgcaata
      841 atcggcaaag tgttatatgt cacaggcacc tacccggaag gcgacgaagc acttgcaaac
      901 cagatcaaag acacaatgga aagcttcgtt gtagcgccta aaaagtaatt gacaataaga
      961 atttattaaa tatactccaa aaaggttaga tacacctttt ttatttgaaa ggagtagcaa
     1021 tatgcgcaag ctgttaattg tcgcgcttct tctgagcata tctttgactg cggtcggctg
     1081 cggatgcggc ggcgaatctg gtggaaacgg caacggtggg aagattgatt acggtgacaa
     1141 gaaccctaat gagggaaaaa tcatcagcag cacaagcacc gatccaaacg cccttaaacc
     1201 ggcaaaggac aaagagcttg ttggcgaaat gaagatcccg ccacacccag ttctaatctg
     1261 gaccctgtat gcttttgaag ggcttgaggt tgcaggagag cagtatcagg gctggacaga
     1321 agtcccgctt acaaaggaaa tgtatgatag cggaacttac aagatgttca cctgggacaa
     1381 aaacccgaag gtaacattta aggtcgttgc atcaaaggac aaggcagtga cgaactcaga
     1441 cccggcgaca ataaagccgc agcttgagaa ctacatagcc tcgatccagg ccacaaaaga
     1501 caaagatggc gaatttaagc acacccagtc cctcataaag ggcatttggc agtcctattc
     1561 ctacaaaaaa gacggggccg attataaggg caaagtcctt gcttactcac tcaggggaat
     1621 cagggtttca gagaccgaat ttggcccagg aaccttcctc aagatcatcg gcgaaggccc
     1681 gtcagcagcc tatgatgaag cagtgaaaga cacaatggat tacatgatcc agcaggtaat
     1741 gatcacccag gtttctgagt gatgattgtt tcgaaatctt caaaggcccc gcgaaagtgg
     1801 ggcctttgtg ttttattggc aacttttttt gcactgtttt tgatctcctg ctccgggcag
     1861 tctgtaatca agtggggtgg ggagagctat tatgtttctt atgacaagaa tttttttggg
     1921 gttgtttccg aagcaaatga cgaattgcag ctcaacatat tggatgtaaa ggatagccct
     1981 ataatcaaaa taattgctgt tccagatttc aagctttctg aaggtgtttt ggtatcggac
     2041 cttcttgatc
//
LOCUS       DAOSRI010000289         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50377_length_2040_cov_4.414610, whole genome shotgun sequence.
ACCESSION   DAOSRI010000289 DAOSRI010000000
VERSION     DAOSRI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50377_length_2040_cov_4.414610"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK717_07585"
     CDS             complement(<1..234)
                     /locus_tag="PK717_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOR47556.1"
                     /translation="MAEKATIVVFSGDLDKVLAAFVIATGCAAQGMEVTMFYTFWGLN
                     VVKKDKGPIAGAKNWMQKMMGMMNRGGISRLPLS"
     gene            complement(402..1274)
                     /locus_tag="PK717_07590"
     CDS             complement(402..1274)
                     /locus_tag="PK717_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOR47557.1"
                     /translation="MIDYIDKIQGAIDFVEAKLKEDVRLNDVAFEAGFSSFHFHRIFL
                     ANVGETVTEYVRKRRLSEAARELMETNRRILDIAVDYQFESQQAFTRAFKKMFFMSPG
                     QCRREKPAICLFLKRCLDREGLKFMRGEKIEVESKILELGEVKAIGLRCYGDVRENGL
                     KQEIKQLWEKFNKVASSVKNISKPGVALGICSMIEGEEPYIMEYVCSMKVDDFSDVPR
                     GMVIRKLSPQKYAVFTHKGKLDKVGQTYDYIYQTWLPQSGCELIDAPDFELYDDRFAP
                     DKDESEFDIYISIK"
     gene            complement(1419..1880)
                     /locus_tag="PK717_07595"
     CDS             complement(1419..1880)
                     /locus_tag="PK717_07595"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOR47558.1"
                     /translation="MEPKIVELGEIKAVGIRYYGDNKNGEIKQVWDKFNKDMCCMKNV
                     SKPGIALGICSMIEGGDPGKFEYVCSMKVDNTSEIPDGMVARVLPPHKYLVFTHKGKL
                     DNLGETYNYIYQNWIPKSGHEIDSSFDFELYDDRFCYGKDESEFDIYIPIK"
BASE COUNT          567 a          482 c          343 g          648 t
ORIGIN      
        1 tgacagtggc aatctgctta tgccacccct gttcatcatc cccatcattt tctgcatcca
       61 gtttttggca cctgctattg ggcctttgtc cttcttgaca acattaagcc cccaaaaggt
      121 ataaaacatc gttacttcca tgccctgggc agcgcaaccc gtggctatga caaaagcggc
      181 cagcactttg tcaagatcac cagaaaacac tacgatggta gctttctcgg ccatgcaacc
      241 tcctttatac ccctatgggg tatttgccac aaaattaact cagaaatata attttgtcaa
      301 tagctcttaa attggttaca ttaaagaaaa agcaatggtg tgattatatg gaaaaagggg
      361 atggaaaacc atcccctcaa ttttttgcta aatccaggat tctattttat cgagatatag
      421 atatcaaatt ctgactcatc cttatcagga gcaaaccgat cgtcgtataa ttcaaaatca
      481 ggagcatcaa tgagttcgca tcctgactgg ggaagccatg tttgatatat ataatcatag
      541 gtttgcccaa ctttatcaag cttaccttta tgcgtaaaaa cagcatattt ttgaggagag
      601 agttttctga taaccattcc tcgaggcaca tccgagaaat catcgacttt catagagcaa
      661 acgtattcca ttatatatgg ctcctcaccc tcaatcattg aacaaattcc aagcgcaaca
      721 ccgggcttac ttatgttttt tacggaactt gccactttat tgaatttctc ccaaagttgt
      781 tttatttctt gtttcaatcc attttctcta acatcgccat aacatctgag tccaatggcc
      841 tttacttcac caagttcgag tatttttgat tccacttcta ttttttctcc tctcatgaat
      901 ttcaggcctt ctctgtctaa gcacctctta aggaatagac atatagcagg tttctcccgt
      961 ctgcactgtc ctggagacat aaaaaacatt tttttgaagg cccttgtgaa agcctgctgg
     1021 ctttcaaatt gatagtcgac agcaatatca agtattcttc gattggtttc catcagctcg
     1081 cgcgcagctt ctgaaagcct tctttttctc acatactcgg tgaccgtttc accgacattg
     1141 gcaaggaaaa tcctgtggaa gtggaaggat gaaaaaccag cttcaaaagc aacatcgtta
     1201 agcctgacat cctctttgag ttttgcttcc acaaaatcta ttgcaccctg tattttgtcg
     1261 atgtagtcta tcatctgcca actatgacac attttatagg agcacgattt ttttgttctg
     1321 atttattttg ctatttcgtg taacaatgat taaaaccaaa agacccccat aactttcatg
     1381 ggggtctttt tttaaacgta ttgaagtggt ttattaaact attttatcgg gatatagatg
     1441 tcaaactctg attcatcctt gccataacag aacctgtcgt catagagctc aaagtcaaac
     1501 gagctatcaa tctcatggcc tgatttaggt atccagttct gatagatgta attgtaggtc
     1561 tcgccaaggt tgtcgagctt tcctttgtgg gtaaaaacaa ggtacttgtg tggtggaaga
     1621 actcttgcaa ccataccatc tggtatctca gaggtattgt caaccttcat ggagcaaaca
     1681 tactcaaact tccctgggtc accaccctca atcatagaac aaattccaag cgctattcct
     1741 ggcttgctaa cattcttcat gcaacacata tctttattaa atttgtccca aacttgcttt
     1801 atctcaccat ttttattatc accataatac ctgattccaa cagcctttat ttcaccaagt
     1861 tcaacaattt tcggttccat tcctactact cctcctctca ggaatcttag gccttccctg
     1921 tccaaacgtc tcctgaaaaa cagatgcaca gcaggtttct cccgtctgca ccgcccagga
     1981 ggtacaaaaa acattttttt gaaggccctt gtgaaagcct gctggctttc aaattgatag
//
LOCUS       DAOSRI010000290         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50550_length_2035_cov_4.741919, whole genome shotgun sequence.
ACCESSION   DAOSRI010000290 DAOSRI010000000
VERSION     DAOSRI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50550_length_2035_cov_4.741919"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>2035
                     /locus_tag="PK717_07600"
     CDS             10..>2035
                     /locus_tag="PK717_07600"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOR47559.1"
                     /translation="MKRTWEITLTIAIIASLTPFTPVVHAQTSLDSSSPQIIGGPMPA
                     RLFLGPTFLHADGSRLYVVEELAVRVQSLFPQNDYLLGTFGFKTGQMFKPGGITTYQG
                     ISYITDTAQSKILVYGKMGEYQGEFKVKDQAQKPVLVNPIGISSYQGRLYIANSGDGN
                     IAVCDTNGKFIQRISKKGTGEGELLYPTGICVASGKIFVTDPEAGKIEVFDQDGNHSI
                     TIPSPRPWAVFPTEGKVFITDISTNKVIMVGIDGTQVGEIQANGFIKTPKGVAFMDGK
                     IYISSYETNKIEIFEVDGKHSGTFGHPVEQAAGTLGRPTGLAIGNDKLYVADSTRNKI
                     IAYSKDGKLSEESKDDIADSKMASPSGVAVDISGNYYVADTANNTIKMFEKDGTFKKA
                     FGGFGSGNGKLNTPSDIIIINPQNKLFVTDTGNNLVQVFSLSGDFLYQFGGFGSAPGQ
                     FNNPIGICTNGTNIFVADSANCRIQELSSDGKPIRTFGHRGRGKDMLLFPMDCSIDNS
                     GKIYVADTYNHRLTVFDPETNFSWNFGKNGGPGDVPYVAEATCDEPPPDKIVYGKFNF
                     PSGIVANDQGCFVSDTYNHRVQFVDFIDIFGDSNYLISPSYVDFGFISPGSIAEKSVY
                     LKNISGGPLTGVVIIPKEAQWLKTHNPTFKSDEEPIVFYVDSNGVPAGSYE"
BASE COUNT          646 a          432 c          423 g          534 t
ORIGIN      
        1 aaattgataa tgaaaagaac gtgggagata acactaacca tagcgataat cgcatcattg
       61 acccccttta ctccagtagt gcatgcacag acttcgctgg attcctcctc cccccaaata
      121 ataggcggac caatgccagc aagacttttc cttgggccaa cattccttca tgcggatggc
      181 tcaaggttgt acgttgttga ggaacttgct gtaagggtcc agtcattatt cccacaaaat
      241 gactatctgt tgggaacttt tggtttcaaa actggccaaa tgttcaagcc gggtggaatc
      301 acaacttacc agggaatatc ctatatcacc gatacagcgc aaagcaaaat ccttgtttat
      361 ggaaaaatgg gtgagtatca aggtgaattt aaagtaaaag accaggccca aaaacctgtc
      421 cttgtgaatc caataggtat ctcttcttac cagggaaggc tttatattgc taactccggc
      481 gacggaaaca tagcagtctg tgacacaaac ggcaagttta tacagaggat atcaaaaaag
      541 ggtaccggtg aaggagaact cctctatcca acaggtattt gtgttgcatc cggcaaaata
      601 ttcgtgactg atcctgaagc agggaaaata gaggtttttg atcaggatgg aaaccattct
      661 ataacaattc cttcccccag accatgggca gtttttccga ctgagggtaa agttttcata
      721 acagacatat caactaataa agtaataatg gtgggtattg atggcacaca agttggcgag
      781 atccaggcca atggtttcat caaaacacca aagggtgtcg cctttatgga tggcaaaata
      841 tatatctctt catatgaaac aaacaaaatc gaaatcttcg aagttgatgg aaaacattct
      901 ggaacatttg gtcatcctgt tgaacaggcc gcaggaaccc ttggaagacc aacaggattg
      961 gccattggaa atgacaagct ctatgttgcc gattcaacca gaaacaaaat tatagcttat
     1021 tccaaagatg ggaaattgtc cgaggaatca aaagatgata tagctgattc gaagatggcc
     1081 tcgccaagcg gcgtagctgt tgatatatca ggaaactatt atgttgctga cactgcaaac
     1141 aacacaataa aaatgttcga aaaagatggg accttcaaga aggcttttgg tggtttcggt
     1201 tccggaaatg gaaagttaaa cacaccatca gatattataa taatcaaccc gcaaaacaaa
     1261 ctttttgtca cagacacagg aaacaatctt gtccaggtct tttctctctc aggtgacttc
     1321 ctctaccagt ttggcggctt tggatcagcc ccagggcagt tcaacaatcc gattggcatt
     1381 tgcaccaatg gcactaatat atttgttgca gactcagcca attgtagaat ccaggaactt
     1441 tcaagcgatg gaaagccaat aagaactttc ggtcacaggg gtagaggcaa agacatgctc
     1501 cttttcccaa tggactgttc aattgacaat tctggaaaga tatatgttgc tgacacatac
     1561 aaccacagat tgaccgtctt tgacccagaa acaaacttct cctggaattt tggtaaaaat
     1621 ggcgggccag gtgatgttcc ctatgtcgca gaagctacat gcgatgaacc accaccagat
     1681 aaaatcgtat acggaaaatt taattttcca tcaggaatag ttgccaatga tcaaggttgc
     1741 tttgtatcag atacttataa tcatagagtg caattcgttg acttcatcga catctttggc
     1801 gattccaact acctcatttc accatcttat gttgattttg gctttatctc tcctggttcg
     1861 atagccgaaa agagtgtata ccttaaaaat atttccggcg ggccactcac cggggtagtc
     1921 ataataccaa aagaagcaca atggcttaaa acacacaatc cgacattcaa aagcgacgaa
     1981 gaaccgattg tcttctatgt tgattcaaat ggtgtccctg caggttctta tgaaa
//
LOCUS       DAOSRI010000291         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50555_length_2035_cov_3.561616, whole genome shotgun sequence.
ACCESSION   DAOSRI010000291 DAOSRI010000000
VERSION     DAOSRI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50555_length_2035_cov_3.561616"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK717_07605"
     CDS             <1..328
                     /locus_tag="PK717_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47560.1"
                     /translation="FVARTGTLYFNELVKLMKLALAKNGFAAIDIFTQCPTNYGRNNR
                     LGKPIDLIKTQASILVPKEKYDSLEQSEKAGKFARGIIQDVEKPTFLDHYRACILSTR
                     KAVHHE"
     gene            321..920
                     /locus_tag="PK717_07610"
     CDS             321..920
                     /locus_tag="PK717_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR47561.1"
                     /translation="MSDFSWKSINHKIFGGKKAESIRFTGSGGHGLVTASVILAKAGV
                     YEGHNVLQSQVYGAEARGGSTKSETVIREGDIYFPKLVNPDVLLALTSEGLEKYRVDV
                     KKDGLIIFDSVLIPDEKSTGDGPFAIGIPMWSELIKELKTGTAINVMSLGLIVGFTEI
                     VGRESLERAVHENFRPEFKTNNQKALDIGYKIAQEVKNG"
     gene            913..>2035
                     /locus_tag="PK717_07615"
     CDS             913..>2035
                     /locus_tag="PK717_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOR47562.1"
                     /translation="MDKYTKIIEQIKALEADMVDFKIVDFYGRLRHVTIPTTNITEKK
                     LQFGFGFDGSNFGFAETGKSDFVARPVIKDFVELDPCLEDPTATFMCDVYEAAQDVKP
                     YESDPRGILRKACAYMQSLGVADVATWGPELEFYLFNDLLVRTDPQAMSAEIISDEGY
                     WGSQDDDYSFATRLPKKGGYHSVPPNDTQYQTRVEAVRRLTSLGYDVKYHHHEVGGPG
                     QCEVEFNLMDSMVAADCIILGKYIIRNTAQEFGSYASFLPKPIFGEAGNGLHVHFLMK
                     GKNGELVFHDPNGYMGLSKMALSFLAGILTHAPAITAITNPSTNSYKRLVRGYEAPCN
                     CCYGMANRNAAVRIPAYATQPEEKRFEYRVGDATFNPYLA"
BASE COUNT          595 a          456 c          484 g          500 t
ORIGIN      
        1 attcgttgcg agaactggaa cactctactt caatgagctg gtgaaactca tgaaattggc
       61 ccttgccaaa aatggttttg cagcgatcga catctttacc cagtgcccaa ccaattatgg
      121 tagaaacaac cgtcttggca aaccaatcga cctgatcaag acccaggcct caattctcgt
      181 gccaaaagaa aagtatgatt cactggaaca gagcgagaaa gccggaaaat ttgcaagggg
      241 cataatacaa gacgttgaga agccgacatt ccttgaccac tacagggctt gcatactttc
      301 tacaagaaag gcggtccatc atgagtgatt tttcctggaa gagcataaac cacaaaattt
      361 ttggagggaa aaaggccgaa tcaataagat tcactggctc tggcggacat ggacttgtaa
      421 cagcctctgt aattcttgcc aaagccggtg tttatgaagg ccacaatgtc cttcagtcac
      481 aggtttatgg tgccgaggca agaggtggct caacaaaatc tgaaacagtc atcagagaag
      541 gcgatatata tttcccgaag cttgttaatc cagatgttct gttggcactg acttctgagg
      601 gtcttgaaaa atacagagtt gatgtcaaaa aagacggtct aataatcttt gattctgtgt
      661 taataccaga cgagaagtca accggtgatg gaccgtttgc aattggaatt ccaatgtggt
      721 ctgaactgat aaaagagctg aaaacaggaa ccgcgataaa tgtcatgtcg cttggattga
      781 tcgttggttt cacggagata gttggcagag aatctcttga gcgtgctgtt catgagaatt
      841 tcagacctga attcaaaaca aacaaccaaa aagccctaga cattggttac aaaatagccc
      901 aggaggtcaa gaatggataa gtacacaaaa atcatcgagc agataaaggc gcttgaggcc
      961 gacatggttg acttcaagat tgttgatttt tatggaaggc tccgccatgt gacaattccg
     1021 acaaccaata tcaccgaaaa gaagctacag ttcggttttg gttttgatgg ttccaatttt
     1081 ggttttgcag agaccggcaa gtctgatttt gttgcccgtc cggtcataaa agattttgtt
     1141 gagctcgatc cttgccttga agatccaacc gcaacattca tgtgtgatgt ttatgaagcg
     1201 gcccaggatg taaaaccata cgaatcagat ccaagaggca tcctgagaaa ggcttgcgct
     1261 tacatgcaat ctcttggggt ggctgatgta gccacatggg gcccggaact tgaattctac
     1321 ctcttcaatg atctgcttgt aaggaccgat ccacaagcaa tgtcggcaga gattatttcc
     1381 gatgaagggt attgggggtc acaggatgat gattactctt tcgcaacaag gctccctaag
     1441 aagggtggat atcattctgt cccaccaaat gacacccagt accagaccag agtcgaggca
     1501 gtaagaaggc tgacttcact tggatatgat gtgaagtacc accatcacga agttggtggt
     1561 cctggccagt gtgaggtgga gttcaacctc atggacagca tggttgctgc tgattgcata
     1621 atccttggaa aatacataat acgcaacacc gcccaggagt ttggctcata tgccagcttc
     1681 cttccaaagc cgatctttgg agaggccgga aacggactcc atgtccactt cctgatgaag
     1741 ggcaagaatg gtgaactcgt gttccacgat ccaaatgggt atatgggtct ttcgaaaatg
     1801 gcactctcgt tccttgctgg aattctcaca cacgcaccgg ccattacagc catcaccaac
     1861 ccgtccacaa actcctacaa gaggcttgtc agaggttatg aggcaccttg caactgctgc
     1921 tatggcatgg caaacagaaa cgctgcagta aggataccag cctacgccac ccagccagag
     1981 gaaaagcgct ttgaataccg tgttggtgat gcaactttca acccatatct ggccc
//
LOCUS       DAOSRI010000292         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50671_length_2032_cov_4.055640, whole genome shotgun sequence.
ACCESSION   DAOSRI010000292 DAOSRI010000000
VERSION     DAOSRI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50671_length_2032_cov_4.055640"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1316
                     /gene="ftsZ"
                     /locus_tag="PK717_07620"
     CDS             243..1316
                     /gene="ftsZ"
                     /locus_tag="PK717_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOR47563.1"
                     /translation="MSSNAFDVWEGSNALIKVVGIGGGGNNAVNRMIECGLEGVEFIS
                     INTDTQTLRMSQAPSKIQIGPKVTRGLGAGSIPDLGEKSAEESKDIIAEMLSGAHLIF
                     VTAGMGGGTGTGAIPVVAQIARDLGALTIAIVTKPFSFEGERRMNVAEGGIEKLKDKV
                     DAMICINNDRLFDATDRNTSLTEAFLMVDEVLRQAVEGITTIITVPGLVNVDFADVRT
                     IMKDAGTAWMGIGTAKGESRAEEAAKMAISSPLLDHSLEGAKGLLCNTIGGKDFKISE
                     ANDVARYITKNIDSKANIVWGAVIDPKMEDQLKVIVIATGFSTQPQNLTIKTPTIKEG
                     DLKNLVSNKRELDIPPFLRKKQQ"
     gene            1316..1984
                     /locus_tag="PK717_07625"
     CDS             1316..1984
                     /locus_tag="PK717_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOR47564.1"
                     /translation="MLSQNYESIKGRIRQVAIATGRDPFSIKILAAVKYTTIDRVNVL
                     FEEGLRIIGENRLQTALRWENHPLRKDFSLHMIGHLQTNKAAKACAIFDTVDTVDSTR
                     LAQNLNKATLRPLPIMVEVNISGEKQKSGCLLQEFDQLCDFIINNCKSLVLSGVFTMT
                     PVDAPDIFREHIFDKADGLAEKLEDRLSTKIERSYGMSDDYELAVKHGSTMVRLGRAL
                     FGGE"
BASE COUNT          610 a          426 c          504 g          492 t
ORIGIN      
        1 gaaacctccc agggcacctt gattgtattt ggtttggcag atgaatttat ggatgttagg
       61 tttggggctc tttctcatta cctcaagtcc cttggaaata gcagccagct cccagccgtt
      121 ataaaacttg atgttcctta tcaagtcaca acatgcccct attaggtcat gttggaaaag
      181 ttgaaattcg ttggcaaaaa gctataattt tcgaagtttt ttgctagaaa aggaaggcat
      241 ctatgtcatc aaacgcattt gatgtttggg aaggctcgaa cgcccttata aaagtagttg
      301 ggattggtgg tggaggcaac aatgcagtca acagaatgat tgaatgtggc ctcgaagggg
      361 ttgaatttat ttccataaac actgacaccc agactcttag gatgtcacag gcaccatcaa
      421 aaatacagat tggcccaaaa gtcaccagag gacttggtgc tggcagcata ccggaccttg
      481 gagaaaagtc tgcagaagag agcaaggata taattgcaga aatgctttcc ggcgctcacc
      541 tcatatttgt caccgcaggc atgggcgggg gtactggcac aggtgcaata cctgtagttg
      601 cacagattgc ccgcgacctt ggtgcgctca cgattgccat tgtcacaaaa ccattcagtt
      661 ttgaaggtga gaggcgcatg aatgttgctg aaggcggcat tgaaaaactc aaggacaagg
      721 ttgatgcaat gatatgcatc aacaacgata gactctttga tgcaactgac cgcaacacga
      781 gccttaccga ggctttcctc atggtagacg aggtgcttcg ccaagcagtg gaaggtatta
      841 cgaccatcat aacagtacca ggccttgtaa acgttgattt tgctgatgtt cgtacaatca
      901 tgaaagatgc tggcactgcc tggatgggta tcggtacagc caagggcgag agcagggccg
      961 aagaagcagc aaaaatggcc atcagttcac cattgctcga tcactctctt gagggagcaa
     1021 aaggtctgct ttgcaacaca ataggtggaa aagacttcaa gatctcggaa gcaaacgatg
     1081 ttgcacgcta cataaccaaa aatattgatt caaaggccaa tattgtttgg ggtgccgtta
     1141 ttgatccaaa aatggaagac cagctaaaag tcattgtcat tgcgaccgga ttttcaacac
     1201 aaccacagaa cctgacaatc aagacaccaa caataaaaga gggggacctc aagaaccttg
     1261 tcagcaacaa aagagaactt gacataccac catttttgcg caaaaaacaa caatagtgct
     1321 ctcgcaaaac tacgaatcca taaagggcag gattagacag gttgcaattg caaccggccg
     1381 ggaccctttt tctattaaga tacttgctgc agtcaaatat acgaccatag acagggtgaa
     1441 tgttttgttc gaagagggat tgaggataat tggtgagaac aggttgcaga ctgctctgag
     1501 atgggaaaac catcccttaa ggaaagattt ttccttgcac atgataggcc acttgcaaac
     1561 aaacaaggct gcaaaagcgt gtgcaatttt tgataccgta gatacggtag actcgacaag
     1621 gctcgctcag aatcttaaca aagcgaccct gagaccacta cccatcatgg tggaggtgaa
     1681 catatccgga gaaaagcaga aatcaggttg tcttctccag gaatttgacc agctttgcga
     1741 ttttattata aataactgta agtctttggt cctctccggc gtctttacga tgacccctgt
     1801 tgacgcgccg gacattttca gggaacatat ttttgataaa gcggatggtc tggccgaaaa
     1861 actggaagac cggctgtcga cgaagatcga gcgctcatac ggaatgagcg acgattacga
     1921 gttggcagtc aagcatgggt caaccatggt aaggcttggc agggcgctgt ttggaggtga
     1981 ataatgggct ttggcgattg gtttaaaaac atactcgggt tgtcagaaga gg
//
LOCUS       DAOSRI010000293         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50674_length_2032_cov_3.602428, whole genome shotgun sequence.
ACCESSION   DAOSRI010000293 DAOSRI010000000
VERSION     DAOSRI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50674_length_2032_cov_3.602428"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..>2032
                     /locus_tag="PK717_07630"
     CDS             63..>2032
                     /locus_tag="PK717_07630"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOR47565.1"
                     /translation="MEKRTVIALAAVSILLASAIAYARPAYYDQLIKTYPSIKGSRID
                     FCLCHERHGGGGPRNMFGQDWAKSGYNFKTIEQTDSDGDGFSNINEIIAQTNPGDPND
                     FPTDKVPPIIAITSPKFEEVITTNTVKITGTATDNERIKEIKVNLQGDFKKANITATG
                     WEAEFFVERGGRKVVKAVAWDMAGNSAEATVEFIVKLTDKSPPKIVFLFPSEGVTVDG
                     LPIVITGTADDDNEVTLVEYSIDGGKTWNKAQGTYSWTVPIPLAPEGEIKVMVRATDD
                     SGNVSQIYTRRFYLKFPEVPAPTISYPQDGMVLDTGTVTISGTIMPPATRVSVRVDGA
                     VSMDAKIEDNFWTTDISGLPPGEHTIEACSFDKYNRKSKECVQVKFVFKLRDTEPPRV
                     QIDSPPDNAEVEIGKISITGTASDNGDIDRVEISFDGTNWMRAKGTTKWSYDYVVNKS
                     QLLRVMARAFDKSGNFTKEPATSTVRVMGPMEVQFVGPDAQMLGDGLLKITVKLTRRP
                     DSTPEIKMTGSTETISIESKDQQTFEISAMLGGGSSYLSVKAEMKGKIYNATKRIDYV
                     VTVKMAIGSTTMIVNGMQKSIPAAATIISGRTYIPFRSIGEALRATVEWDDKRKQATY
                     KLGTNTYYLTLGQTKAYVNKTEVKLSSAPLLVG"
BASE COUNT          678 a          445 c          487 g          422 t
ORIGIN      
        1 tatcacctag taaaagcaac ctttaaactt gccaaaattt ccataagcgt tacatttgtg
       61 tcatggaaaa aagaacagtt atcgcattgg ctgcggtaag tattctgctt gcctctgcaa
      121 tcgcatacgc acgacctgct tactatgacc aattaataaa aacctacccc tctataaaag
      181 ggagcagaat agatttctgc ctctgtcacg agagacacgg cggtggaggg ccaagaaata
      241 tgttcggcca agactgggcc aagagcggct acaatttcaa gacaatcgag caaacggact
      301 ctgacggaga cggtttttca aacatcaatg aaatcatcgc ccagacaaac cctggcgacc
      361 cgaatgattt tccaaccgac aaagttccac caataatcgc aataaccagc ccaaaatttg
      421 aagaagtgat caccaccaac accgtaaaaa taacaggaac cgcaacagac aatgagagaa
      481 taaaagaaat aaaggtaaat cttcagggag atttcaagaa ggcaaatatc actgccactg
      541 gctgggaagc agaatttttt gtagagagag gtggcaggaa ggtagtaaag gcagttgcct
      601 gggacatggc gggcaattcg gcagaggcaa ccgttgagtt tattgtcaaa cttacagaca
      661 aatctccacc aaaaatcgtt tttctgttcc catccgaggg cgtgaccgtc gacggcctgc
      721 caatagtcat cactggcaca gcagatgatg acaatgaagt aacacttgtg gaatattcca
      781 tcgatggtgg caaaacatgg aacaaagcgc aaggaacata tagctggaca gtaccgatac
      841 cacttgcgcc agagggcgaa ataaaagtaa tggtcagggc aactgatgat tcagggaatg
      901 tcagccagat ctatacaaga aggttttatc tcaaatttcc tgaagtgcca gctccaacaa
      961 tatcctatcc acaggatggg atggtcctgg acacaggcac agtaactatt tccggaacaa
     1021 tcatgccacc ggcaaccagg gtttcggtaa gggttgacgg ggcggtgagt atggacgcca
     1081 agatagaaga caatttctgg actacagaca tatccgggct accaccaggc gaacacacaa
     1141 tcgaggcttg ttcatttgac aaatacaaca gaaaatccaa ggaatgcgta caggtaaagt
     1201 ttgtattcaa gctaagagac acagaacccc caagggtgca aatagattca ccgcccgata
     1261 atgcagaggt cgaaataggg aaaatatcta tcacaggcac cgcaagcgac aatggcgaca
     1321 ttgacagggt ggagatttct tttgatggca caaactggat gagggccaag ggcaccacaa
     1381 aatggtctta cgactatgtt gtcaataaat cacaattgtt gagggtgatg gcaagggcat
     1441 ttgacaagtc tgggaatttc acaaaagaac ctgccacttc aactgtcagg gtcatggggc
     1501 ccatggaagt gcaatttgtt gggccagatg cacagatgct tggcgatggg ttgctgaaaa
     1561 tcacagtgaa actgacaagg aggccagaca gcacgccaga aataaaaatg accggctcaa
     1621 cggaaacgat aagcattgaa tccaaagacc agcaaacatt cgaaatcagc gcaatgcttg
     1681 gcgggggatc gtcctatctg tcggtaaaag ctgaaatgaa gggcaagata tataatgcca
     1741 caaaacggat agattatgtt gtgacagtca aaatggccat tggaagcacg accatgattg
     1801 tcaatggaat gcagaaatca ataccagccg ctgccacgat aatatctggc agaacatata
     1861 ttcctttcag atcaattggc gaggcactga gggcaactgt tgaatgggat gacaagcgga
     1921 aacaggccac atacaagctg ggcacaaata catattacct gacccttgga caaacaaagg
     1981 cctatgttaa caaaacagag gtgaaacttt cgtcggcacc gcttcttgtc gg
//
LOCUS       DAOSRI010000294         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50707_length_2031_cov_3.451923, whole genome shotgun sequence.
ACCESSION   DAOSRI010000294 DAOSRI010000000
VERSION     DAOSRI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50707_length_2031_cov_3.451923"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK717_07635"
     CDS             <1..401
                     /locus_tag="PK717_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47566.1"
                     /translation="FWVVIWILPDVPTKSIRSYFSAIMDQDYQKAWSYIYPNSEFMRD
                     RGGPTMSFEKFRDDLTNARSHGTKMTEFKILKVYEQQDPLAKITAQIVRIQTENIVSG
                     NPKTSDPKDYYLRKDKDGVWKIYKGQIPKE"
     gene            365..904
                     /locus_tag="PK717_07640"
     CDS             365..904
                     /locus_tag="PK717_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47567.1"
                     /translation="MENLQGTDTKRINRSESTLAGPLTWFLLLIVGLTFIGSVYTIFQ
                     DSPARAIRNYFKLCFDGKYEQAWNTYVLEGSNFQKDKGGDLKTFSETWERSKNHGTDY
                     LNVRIDGVKAITQSTGNKKMVTVRFSLMQYDEDTKEKESGNPKAGMVKVKVLQDSYSG
                     NLVLQHTPGKSWQIVSIGQ"
     gene            906..1928
                     /locus_tag="PK717_07645"
     CDS             906..1928
                     /locus_tag="PK717_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47568.1"
                     /translation="MKKTLVIFLLVVVFVVGFGAYTAFAGFWIEGLITDSSSGKPVEG
                     SIVTIAGVSTTTDNQGKFARWLAPFPSTPNYIFVEHSLYQPFLKYFDGVDFSKPIEIK
                     LDPETYEGHLVKARDVINKLNGFVLETTSTFYEKDKKTGKVTINKGEAIYGLTENTRY
                     FVQIYSNNIKGELSKSETIIEDSDHSLLDTISATKSGASPIIYYVDDQIKDWIKFKLI
                     DDPVFQIPMPEVNPRKMLEPLMSFGNTKIMTPMADAGKSADGEKLIGATLSWDPKSVL
                     VGKTVQIKYRPNGLWYEIVFIDTGENPASKPGKYTFTLLSYDQFTSIKKPANAKEITP
                     SELIGK"
BASE COUNT          675 a          417 c          433 g          506 t
ORIGIN      
        1 ggttttgggt ggttatctgg attcttccag atgtgccaac aaaatcgata aggagctatt
       61 tcagtgcgat aatggatcag gactaccaaa aagcctggtc ctacatctat cccaattcag
      121 agttcatgag ggacaggggc ggtccaacaa tgtccttcga aaaattcagg gatgatctca
      181 ccaatgccag aagtcatggc accaaaatga ctgagttcaa gattttgaaa gtttacgaac
      241 agcaagaccc acttgctaag ataactgccc agattgtaag gatacaaaca gaaaacatag
      301 tcagcggcaa tccaaaaact tctgatccca aagactatta tctgaggaag gacaaggatg
      361 gagtatggaa aatctacaag ggacagatac caaaagaata aacaggagtg aatcaacact
      421 tgccgggcca ctcacatggt ttctcctgct gattgtaggc ctgacattta ttggctctgt
      481 atatacaatt ttccaggact cgccggccag agccattagg aactatttca aactctgttt
      541 tgatggaaaa tacgagcaag cgtggaacac atatgtattg gaaggatcta atttccaaaa
      601 agacaagggt ggcgatctaa aaacattctc cgaaacctgg gaacgctcaa agaatcatgg
      661 tactgactat ctcaatgtta gaattgatgg tgtaaaggca attacccaga gcactggcaa
      721 caaaaagatg gtcactgtta ggttcagcct catgcagtat gacgaggaca caaaagagaa
      781 ggaatcaggg aatccaaaag ccgggatggt aaaagtaaag gttctccaag actcttatag
      841 cgggaacctt gtgctacaac acacccctgg aaaatcctgg caaatagtta gtatcggaca
      901 ataaaatgaa aaaaacactt gtaatatttc tcctggttgt agtttttgtt gtaggttttg
      961 gtgcttatac agctttcgct ggtttctgga ttgagggcct gataacagat tcgagctctg
     1021 gaaaacctgt tgaaggttcc attgtaacaa tagcaggcgt gagcaccaca accgacaacc
     1081 aggggaaatt tgcaaggtgg ctagcacctt ttccttcaac accaaactat atttttgtcg
     1141 agcattcact ttatcaacct tttttaaaat attttgatgg agtcgatttt tcaaaaccca
     1201 tagagatcaa gctcgatcct gaaacctacg agggccatct cgtgaaagca agagacgtca
     1261 ttaacaagct taatggtttt gttttagaaa caacaagcac tttttacgaa aaagacaaga
     1321 agactggcaa ggtgactata aataagggtg aggccatcta tggcctaaca gagaacacca
     1381 ggtattttgt ccagatttac tccaataaca tcaagggaga gctctcaaaa tcagaaacaa
     1441 ttattgaaga ttctgatcac agcttacttg atacgatatc cgcaacaaaa tctggcgcct
     1501 caccaattat ttattatgtg gatgaccaga taaaagactg gataaaattc aaactgattg
     1561 acgacccagt ttttcagata ccaatgccag aagtaaaccc gagaaaaatg cttgagccat
     1621 taatgtcttt tggcaatacc aagataatga cgccaatggc ggatgccggc aaatctgcgg
     1681 atggggaaaa acttattgga gcaactctca gctgggatcc aaaatcagtt cttgttggaa
     1741 aaacagtcca gataaaatat cgcccaaatg gtctctggta tgagatagtc tttatagata
     1801 ctggagaaaa tcccgccagt aaaccaggca aatacacttt cacactgctt tcatatgatc
     1861 aattcacgag cataaaaaaa ccagccaatg ccaaggagat aactccctcg gagctcattg
     1921 gcaaataagg aggtagctgt tgcccctgct tgatgttttt acccagactt tgcggtcaat
     1981 tggattcgct gcaataccaa aagtgggcgg cacaatacca gttcctgaaa c
//
LOCUS       DAOSRI010000295         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_50849_length_2027_cov_2.588235, whole genome shotgun sequence.
ACCESSION   DAOSRI010000295 DAOSRI010000000
VERSION     DAOSRI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50849_length_2027_cov_2.588235"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK717_07650"
     CDS             complement(<1..350)
                     /locus_tag="PK717_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOR47569.1"
                     /translation="MKKTLAFLIALSILAPVASALAIDGTITVKPLESDVLTSSEIKV
                     DIRASGLKDVYAMSFELEYNNRLVQFKEIEEGALLNADPKPLFQTKVTSTKDGGKIFV
                     GLSNIDGKTNLKDQG"
     gene            complement(350..1156)
                     /locus_tag="PK717_07655"
     CDS             complement(350..1156)
                     /locus_tag="PK717_07655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOR47570.1"
                     /translation="MMEMVCLDLDGTLLPPTGPMTERNIECLRDAHKKGVKIVIASGR
                     RFGSIEPHARQLGFNMPIIAYSGAWVKNLDDEEPTIKSNTPWEPTVELIKKLKGNVDL
                     IGIYVDDMLYLDDANEYSERYEKRVSLKAVLVDNLSEHFENKKQDITKMLVVENKTDY
                     LDSIYTQMKKEYAGMLDFVLSWSTFMEVGTVGVSKGGALSVWAEQNGIDLSRSVAFGD
                     QENDLSTFGVCGVSVAMGNATDQVKEMANIVAPPNSEDGVAQVLERMLAC"
     gene            complement(1153..>2027)
                     /locus_tag="PK717_07660"
     CDS             complement(1153..>2027)
                     /locus_tag="PK717_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOR47571.1"
                     /translation="ETVTDWLSSLTNGVSPETLNTALNSVFEKLQSSLGAISGFIVKG
                     LADIATAAAGILFQAFIILIISTFFLLDWRYINAAMFAMLPNNTSRNNIKKLFASIYT
                     QIWTYVKTQFLLSLFTGTMISLLCFILGIPSALIIGIIVALGEMVPYIGPLFSFSIGT
                     LLAAVAALPTPINPSGSFYILIWYVIGFLAIEQGLTQAIATPVLAKKAQTHPLLVIIV
                     MFSFYNLFGPLSVLLAVPFLVTLKAMFAYLTSENKLFKRLGIDLESYYQSNATSPLIA
                     RLRRPFSTKGPLSK"
BASE COUNT          558 a          432 c          440 g          597 t
ORIGIN      
        1 ccctggtctt tgaggtttgt ttttccgtca atgtttgaaa gccctacaaa aatctttccg
       61 ccatcctttg ttgatgtgac ttttgtctgg aacagtggtt tggggtcagc attaagaagt
      121 gctccctctt ctatctcctt gaactggaca agtctgttgt tgtattccag ctcaaaagac
      181 attgcatata cgtcttttaa gccagatgcc cttatgtcca cttttatctc agagctggtt
      241 aaaacatcgg attcaagcgg tttgacagtt attgtgccat ctatggccag cgccgaagct
      301 accggagcaa gtattgacaa cgcaatcaaa aaggccagtg tttttttcat tagcaagcga
      361 gcatcctctc tagtacctgg gccactccat cttcggagtt tggcggggcg actatattgg
      421 ccatttcctt gacctgatct gttgcatttc ccattgcaac agacaccccg catacaccaa
      481 atgtggagag gtcgttctcc tggtcaccaa acgccactga tcttgaaaga tcaatgccat
      541 tctgttctgc ccaaaccgag agtgccccac cttttgagac gcccacagtt ccgacttcca
      601 tgaatgttga ccatgaaaga acaaaatcca gcatcccagc atactctttt ttcatctgtg
      661 tataaatact gtccaagtag tcagttttgt tctcaacgac caacattttg gtgatgtcct
      721 gttttttgtt ttcgaaatgt tctgagagat tatcgaccag aacagctttt aatgagactc
      781 ttttttcata tctctcggaa tattcatttg catcatcaag atatagcata tcatcgacat
      841 aaatgcctat taaatcgaca ttccccttta gcttttttat taattctaca gttggttccc
      901 aaggggtatt gctttttatt gtcggttctt catcatcgag gtttttgacc cacgctccgg
      961 agtaggcgat tattggcata ttgaatccca attgtcttgc atgtggctca atggatccga
     1021 agcgccttcc tgaggcaatc acaatcttga cgcctttttt atgggcatcc cttaaacatt
     1081 ctatgtttct ttcagtcata gggcctgtgg gtggcaaaag agtaccatcc agatcaaggc
     1141 agaccatttc catcattttg agagagggcc ctttgttgag aaaggacgcc ttagcctggc
     1201 tataagtggt gaggtggcat tggattggta ataggactca aggtcaatcc caagcctctt
     1261 gaaaagcttg ttttcagatg tcaggtaagc aaacattgct tttagggtta ccaggaaagg
     1321 cactgcaagc aaaaccgaca atggcccaaa aagattgtag aaagaaaaca ttacaatgat
     1381 gacaagaagt gggtgtgtct gtgctttttt tgcaagcact ggggttgcaa tggcctgggt
     1441 aaggccctgt tcgatggcaa gaaatccaat cacataccaa ataagaatgt aaaaggaacc
     1501 actaggatta attggtgttg ggagcgctgc aacagcggca agcaaagtac ctattgagaa
     1561 tgagaaaagt ggaccaatgt atggcaccat ttcaccgaga gcaacgatta tgcctattat
     1621 cagggcagaa ggtataccaa gaataaaaca caacaaactt atcatggtgc cagtaaaaag
     1681 ggaaagcaaa aattgtgttt ttacatatgt ccagatttgg gtataaatgg aagcaaataa
     1741 tttttttatg ttatttcttg aagtgttgtt tggcagcata gcaaacattg ctgcattgat
     1801 gtatctccaa tccagcaaga aaaaagttga aataatgagg atgataaaag cctgaaaaag
     1861 aatcccggca gcagcggttg ctatgtctgc gagccctttg acgatgaatc ctgatattgc
     1921 gcccaacgaa gattgcagtt tttcgaaaac cgaatttagg gctgtgttga gggtttccgg
     1981 actcacacca tttgttaggc tgctcagcca gtcggtgaca gtttcct
//
LOCUS       DAOSRI010000296         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_51523_length_2010_cov_3.342711, whole genome shotgun sequence.
ACCESSION   DAOSRI010000296 DAOSRI010000000
VERSION     DAOSRI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51523_length_2010_cov_3.342711"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PK717_07665"
     CDS             complement(<1..1255)
                     /locus_tag="PK717_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47572.1"
                     /translation="MAVWKKIAVLFVLPLCFASLSGAVYSAEERYTPVKAIGYGPLAG
                     ELIRPTAVGIDESSGAWIYVVDSASKTCSIYMQNGILNKSFAIPDYIDLSRIKSNISA
                     SEGIVCYITGSEIIRANRTGEILTKIRIGSESIVNPIIARLNSDQSISVIDSVSGLLK
                     ISDKGKLVSRFIHIGKDVTTIDGGGINKDGSVAIITITPPTIGEEPVDKIPFNSIKIT
                     VFDKNGQKELEFPVETSQSFNPSAGQVRFDPKGNICFMGNNSSIVRVYDKTGSKISEF
                     AIAGFGSVPLAFCVAFNGNIYAVDGSKFFATNSQGKYLGEFGAFGGKSTQFGNPVDLA
                     VCGQNGFAVFDSHRNDMQFFGQNGVTGTRQYGQNAVKLTLSTSQGGSILAYNPSNSTL
                     RTYDCSGNPSTDVVNVDKVSQNIASF"
     gene            complement(1315..1956)
                     /locus_tag="PK717_07670"
     CDS             complement(1315..1956)
                     /locus_tag="PK717_07670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOR47573.1"
                     /translation="MSRITRLILVRHGESTGNSEGILCGWHDSSLTEKGIAQAERVAL
                     VLKDAGINAIISSDLQRAKQTSLIISKQLGLDVCFDERFRERACGIFDGKKIDDLKNH
                     ESWENFVNDSNFTLEGGESIVDFSKRISESCEMIAREHEGENILLISHGGVLWAMVPY
                     VLGVSASSYNGLIGMDNCSISVFSRHDENWVLQTLNSTQHLGGAFLDAHSWRL"
BASE COUNT          606 a          464 c          349 g          591 t
ORIGIN      
        1 aaaatgaagc aatattttga gataccttgt caacatttac cacatctgtg gagggatttc
       61 cagaacaatc ataagttcta agggtgctat ttgaaggatt atatgcaagt atactccctc
      121 cctgagaggt tgaaagtgtc agtttgacag cattttgtcc atattgtctt gtcccggtaa
      181 caccattctg gccaaaaaat tgcatgtcat ttctgtggga atcaaaaact gcaaaaccat
      241 tttgcccaca aaccgcaaga tcaacaggat tcccgaactg tgttgacttg ccaccaaatg
      301 caccaaattc accaaggtat ttaccttgag aatttgttgc aaaaaatttg gagccatcaa
      361 ctgcatagat attcccatta aaagcaacgc aaaaagccag aggcacagaa ccaaatcctg
      421 caattgcaaa ttctgaaatt ttgctgcctg ttttgtcata aactcttact atagaagaat
      481 tattgcccat aaaacaaata ttaccttttg ggtcaaatcg tacttgtccg gcagatggat
      541 tgaagctttg actggtttct accggaaatt caagctcttt ctgtccattt ttgtcaaaaa
      601 cagttatttt tattgaatta aatggtattt tatctacagg ctcttcgccg attgttggag
      661 gagttatagt gattatggct actgatccat ctttgtttat accaccacca tcaattgttg
      721 taacatcttt gcctatatga ataaatctcg atacaagctt ccctttatcc gaaatcttga
      781 gtaaaccgga caccgagtct ataaccgata ttgactggtc agaattcaat ctggcaataa
      841 tcgggttaac aatgctctca ctgccaattc ttatcttggt aagaatttct ccagtcctgt
      901 ttgctcttat aatctcactt ccagttatat agcagacaat gccttctgaa gcagagatat
      961 ttgattttat cctgcttaaa tcaatataat ctgggatcgc aaaagatttg ttcaaaatgc
     1021 cattttgcat gtatatagaa caagttttgc ttgcagaatc gacaacatat atccaggcac
     1081 cagatgattc atcgatgcct actgcagttg gccttatcaa ttcacccgca agagggccgt
     1141 agccaatcgc ctttactgga gtgtatcttt cctcagcgga gtaaacggcc ccggacaaag
     1201 aagcaaaaca caagggcaga acgaacaaga cagcaatttt tttccaaaca gccatgttac
     1261 ctcctgtaac acttaccatt ttattataca ccaagttcaa atggtaatgt aaagtcacaa
     1321 cctccacgaa tgcgcatcca gaaaagcacc acccaggtgc tgcgtagagt taagtgtttg
     1381 caggacccag ttctcatcat gccgtgagaa aacgcttatc gagcaattgt ccatccctat
     1441 caaaccatta taggagctgg cagagacacc aaggacatat ggcaccattg cccaaagtac
     1501 accgccatgg gagatgagca gaatattttc tccctcatgt tcacgagcaa tcatttcgca
     1561 ggattcagat attctctttg aaaaatcgac aatgctttcc ccaccctcca atgtgaaatt
     1621 tgaatcgtta acaaaatttt cccaggattc atggtttttc aaatcatcaa tctttttgcc
     1681 atcaaatata ccacatgcac gttccctgaa cctctcatca aaacaaacat ccagcccaag
     1741 ttgttttgaa atgatgaggg acgtttgttt tgccctctga agatcagagc tgattatggc
     1801 atttatgccg gcatccttca ggaccaaggc aacccgttct gcctgggcaa ttcccttttc
     1861 cgtaaggctg gaatcatgcc aaccgcacag aattccctcg ctgtttcctg tactttcacc
     1921 atgcctgacc aaaatcaatc ttgttattct cgacattttt gctcctgtga ttttatgtta
     1981 taatacaaac tacaaaacaa aaacgtcaag
//
LOCUS       DAOSRI010000297         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_51731_length_2004_cov_3.853258, whole genome shotgun sequence.
ACCESSION   DAOSRI010000297 DAOSRI010000000
VERSION     DAOSRI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51731_length_2004_cov_3.853258"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PK717_07675"
     CDS             complement(<1..409)
                     /locus_tag="PK717_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOR47574.1"
                     /translation="MGLQVAVFGLGRFGMALATEVERKGYGVLAVDTDPEKVNQIEGL
                     VSSAIRLHTINEQELRDSGVTQCDVAVIGFAKNIESNILLTQTLKDLGVGFIIARAES
                     AIHARVLQKIGADDIFFPEQDMAVRIANRITGTS"
     gene            490..651
                     /locus_tag="PK717_07680"
     CDS             490..651
                     /locus_tag="PK717_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOR47575.1"
                     /translation="MRIKIIAKKPACSNIDDENCRECQTPCKSAAKTANTISNQICEA
                     KGANSPWIL"
     gene            723..1943
                     /locus_tag="PK717_07685"
     CDS             723..1943
                     /locus_tag="PK717_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR47576.1"
                     /translation="MDDEAHDLFTKIEEGFDPFSLPPGEAREMVLGLISEGIILSKVD
                     YPKPQKLSPKSLCLIVSHACNMACTYCSLSKATIEGKLMSPETAIKAMKWLIDNAPGT
                     KVDVDFFGGEPTLAWKTVKAVVDFSKEYSKTKSKTVRFSMSTNALGLTDEMLDYCDEN
                     YISLILSLDGPKAENDRCRVLKDGRPSFDTVLPNIMKVAQRRQRGWYVRGTYSKETIN
                     FSDSVIALHKMGINNLAFEPVVSNDPKVAFSEKDIPAIREEYEKLARYYVECKKTDMP
                     IKFYHFEMDLENGPCARKSAGGCGAGAEYLAVSPDEKIWPCHQFDTEEGFCLGTLDNP
                     PTQAIYDEYTIKNHLTEKRECPDCWASYLCSGGCIASNKLQEGDVLLPYKMGCLIQKI
                     RLEAALWVYSELRE"
BASE COUNT          549 a          468 c          464 g          523 t
ORIGIN      
        1 acgaagttcc cgttatccta ttggctatcc tgacggccat gtcctgctcc gggaagaaga
       61 tgtcgtctgc cccaattttt tgcaggaccc tggcatggat ggcactttcc gctctggcaa
      121 tgatgaagcc gactccaagg tcctttagcg tttgtgtcag gaggatgttt gattcgatgt
      181 tttttgcaaa accgattact gcaacatcgc attgggtcac acctgagtcc ctgagttcct
      241 gctcgttgat tgtgtgcaat ctgattgcac tggaaacaag tccttcaatc tggtttacct
      301 tttcagggtc ggtgtcaacc gcaagtaccc cgtagccctt tctttcaact tctgtggcca
      361 gggccattcc aaaccggcca agcccgaaca ctgcaacctg aagacccata aggcacctcc
      421 attgccaaat ataacacaat ccttgacagc ataaaggggg catttacaat gcttccggag
      481 gtaacaagaa tgcgtattaa aataattgca aagaaacctg cctgcagcaa cattgatgac
      541 gaaaactgca gggaatgcca gaccccgtgc aaatcagcag ccaaaactgc aaacacaatt
      601 tcaaaccaga tttgtgaggc caagggggca aattctccct ggatactcta ggtaaaatcc
      661 acgctttcaa gtttaacgaa aagaccctgc tttatgattg ccaagcgggg tctttgcttt
      721 tattggacga tgaggcccac gatcttttta caaaaataga agagggtttt gatccgttta
      781 gtttgcctcc cggcgaggcc agggaaatgg ttttagggct gatcagtgaa ggcataattc
      841 tttccaaggt tgattatccg aagccacaga agttgtcccc aaagtctttg tgtctcatag
      901 tttctcatgc ctgcaacatg gcgtgcacct attgttccct ctccaaggct accattgagg
      961 gcaagctgat gtctcctgaa acagcaatca aggccatgaa atggctgatt gataatgccc
     1021 ctgggaccaa ggttgatgtg gacttttttg gtggagagcc cacgcttgcc tggaaaactg
     1081 tcaaagcggt ggtcgatttt tccaaggaat attcaaaaac caagagcaaa acggtgcgtt
     1141 tttccatgtc cacaaatgcg ctgggtctca ctgacgagat gcttgattat tgcgatgaaa
     1201 attacatatc gctcattcta agtcttgatg gcccgaaagc tgaaaatgac agatgcaggg
     1261 tactaaaaga tggcaggcca tcttttgata ctgttttgcc caatatcatg aaggttgccc
     1321 aaagaaggca aagagggtgg tatgtaaggg gtacatattc aaaagaaacc atcaactttt
     1381 ctgattctgt cattgccctt cacaagatgg gcatcaacaa cctggctttt gagccggttg
     1441 taagcaacga cccaaaagtt gctttttcgg aaaaagacat ccctgcaatc agagaagaat
     1501 atgaaaaatt ggccagatat tatgttgagt gcaaaaaaac tgacatgcca ataaagtttt
     1561 accactttga aatggacctg gaaaatggcc cctgtgcaag aaagtctgct ggtggttgtg
     1621 gcgccggcgc agagtatctg gcggtatcgc ctgatgagaa gatttggcca tgccaccagt
     1681 ttgacaccga agagggtttt tgccttggaa cgcttgataa cccacccaca caggccattt
     1741 atgacgaata taccatcaaa aaccatctga ctgaaaaaag agaatgccct gactgttggg
     1801 cctcatatct ttgttctggt ggctgtattg cctcaaacaa attgcaagag ggcgacgttc
     1861 tcctgcccta caaaatgggt tgcctcatcc aaaagataag gctggaagcc gccctctggg
     1921 tttattctga actcagggag taaaaacctc ttttacaagc acctcaagct cgtccatcgt
     1981 tttgtccagc tcgtaggtcg ttgt
//
LOCUS       DAOSRI010000298         2000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_51893_length_2000_cov_3.688432, whole genome shotgun sequence.
ACCESSION   DAOSRI010000298 DAOSRI010000000
VERSION     DAOSRI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51893_length_2000_cov_3.688432"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK717_07690"
     CDS             <1..253
                     /locus_tag="PK717_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47577.1"
                     /translation="ELDYYNLGAKKLAEKLNLTVPKTLAVINHLEIQSNADYFKEMHH
                     NKTIIKGYSQKALEKIRKTLDEVSIEEIWTNYSKRNIKQ"
     gene            250..>2000
                     /locus_tag="PK717_07695"
     CDS             250..>2000
                     /locus_tag="PK717_07695"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOR47578.1"
                     /translation="MIIKSLTESEVEEVAMEYLWTTGWLTAKGPNIAPDGASAERQNY
                     SEVVFSRRLLDSLASLNPKVPADAIEDAFRKITRPDSPSLVQCNRMFHRMLTEGVDVE
                     YQANGRTVHDKVRVADFEKPENNNFLAVNQFTVVEGQHNRRADIVLFLNGLPVAVVEL
                     KNVADENATIWGAFNQLQTYKNQIPSLFTFNEFLVISDGLAARVGSLTADRERFSPWK
                     TIEGKEPASSRMPQLEVLFKGLFDKKRFLNFLRHFVVFEDEGNGKVIKKIAGYHQFHA
                     VNEAVQETIRACNCKITTSQHGDSKPGNHRVGVVWHTQGSGKSLTMAFYAGRIVLCPE
                     MENPTLVVITDRNDLDDQLFGTFSRCHELLRQKPIQAESRVHLCEMLNVASGGIIFTT
                     IQKFFPEEKGDVFPKLSNRQNIVVIADEAHRSQYDFIDGFARHMRDALPNASFIGFTG
                     TPIDLTDKNTRAVFGDYISIYDIQRAVEDGATVPIYYEGRLAKLELNEAEKPHLDEEF
                     EEITEGEEAAQKEKLKTKWAALEAVAGTEKRIKLIAKDIINHFEHRLEAMDGKAMIVC
                     MSRRICVDLYNAIVTLRP"
BASE COUNT          614 a          454 c          471 g          461 t
ORIGIN      
        1 tgaactcgat tattataatc tcggagcaaa aaaactagca gagaaattaa accttacggt
       61 acccaaaacg ttagcagtca taaatcattt agaaattcaa tcaaatgcag actattttaa
      121 agaaatgcat cataataaaa caattatcaa aggatactcc cagaaggctt tagaaaagat
      181 aagaaaaaca ttagatgagg tttctattga agaaatttgg acaaattatt ccaagaggaa
      241 tattaaacaa tgattattaa atccctcacc gaatctgaag tcgaagaagt cgcaatggag
      301 tatctctgga ccactggctg gctgacagca aagggcccga atatcgcacc agacggggca
      361 agcgccgaga gacaaaacta cagcgaagtc gtcttttcca ggcgcttgct tgattccctg
      421 gcctctctca acccaaaggt cccagcagac gcaatagaag acgccttccg caagatcacc
      481 aggccagact cgccctcgct tgtccagtgc aacaggatgt ttcaccggat gctcaccgaa
      541 ggggttgatg ttgagtacca ggccaatgga cgaacagtcc acgacaaggt gagggtggcc
      601 gattttgaaa aaccggagaa caacaatttt ctggcagtga accagtttac cgttgtcgag
      661 ggccagcaca accggagggc tgacattgtc cttttcctga acggtttgcc tgtcgccgtt
      721 gtggagctaa aaaacgtggc agacgagaac gcaaccatct ggggtgcctt caatcagctc
      781 caaacctaca agaaccagat tccgtcacta ttcactttca acgaatttct ggtcatctcg
      841 gacgggctag cggcaagggt gggatcgctc acagcagacc gggagagatt ttcaccctgg
      901 aaaaccattg aaggcaagga gcctgcatct tcaagaatgc cacagctgga ggttttgttt
      961 aaaggacttt tcgataaaaa gcgttttttg aattttttgc ggcactttgt tgtttttgag
     1021 gacgaaggca acggaaaggt cattaaaaaa atcgccggat accaccagtt ccatgcagtc
     1081 aatgaagctg ttcaggaaac aatccgcgcc tgcaattgca aaattaccac aagccaacat
     1141 ggggacagca agcctggcaa ccaccgtgtc ggagtggtct ggcacaccca ggggtccggg
     1201 aaaagcctga caatggcttt ttacgccgga cgaattgttc tttgcccaga aatggaaaat
     1261 ccaacactgg ttgttatcac ggatcgcaac gatctggatg accagctgtt tggaacattt
     1321 tcacgttgcc acgagctttt gcgccaaaaa ccgatccagg cagagagccg tgtccacttg
     1381 tgcgaaatgc tgaatgtggc ttctgggggt attattttta caacaatcca gaaatttttc
     1441 ccagaagaaa aaggtgatgt attcccaaag ctttccaaca gacaaaacat cgtggttatt
     1501 gcagatgagg cccaccggag ccaatatgat tttattgatg gttttgcacg acacatgcga
     1561 gatgccctgc caaacgcttc ttttattggt ttcactggaa ccccaattga tttgactgac
     1621 aaaaacactc gggcagtttt cggagattat atcagtatct acgatatcca acgcgcggta
     1681 gaagatgggg caactgtgcc catctattat gaaggtcgtc tagccaaact ggaactcaac
     1741 gaggcagaga aacctcactt ggacgaggaa tttgaagaaa taacagaagg tgaagaggca
     1801 gcacaaaaag aaaaactaaa aaccaaatgg gcggcactgg aggctgtagc cggaacagaa
     1861 aaacgcatta agcttattgc caaagacatt atcaatcact tcgagcaccg ccttgaagcg
     1921 atggatggca aggcaatgat tgtctgtatg agccgacgta tttgtgttga cctttacaat
     1981 gctatcgtga ccctgagacc
//
LOCUS       DAOSRI010000299         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_53202_length_1966_cov_3.739403, whole genome shotgun sequence.
ACCESSION   DAOSRI010000299 DAOSRI010000000
VERSION     DAOSRI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53202_length_1966_cov_3.739403"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PK717_07700"
     CDS             <1..1441
                     /locus_tag="PK717_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOR47579.1"
                     /translation="NVPIAWSVEPAGLGIFDANGKFTAGAIAGTGTVKACSAGVCGTS
                     TITVMASGQIETITVSPSNITMNMGETQAFKAIVKDKNGFEISGAPISWSVNPPDYGT
                     ISTNGVFIASKPGMCVIIAQSGDKSGTAIVNIVQVTRIDLTPKSVIIAPNTTQQFTAV
                     AYGQTGQQIPGIKFNWTVTPQTLGAISESGLFTALGSPGQAGLITVSAAGMTATATVT
                     IGIPDNTPPRITLVKPTINGGNSPIGPSTVEVKVEDESGVSEVTINGVPATMNANGNW
                     AANVIVNPGNNTFMVKAKDNSTSRNEATLAVTIYGATESRIQMRIPTNNQADVKTVTI
                     TENNVPRTITLKTSPELYQNSTFVGLRDLAENLLGATPEKGGSVAYDGATRKITITIK
                     RTNGDVMIFETVVGEKDYQLQTIHPDGTREIKTEVMAQAPYIGSAATKSRKVNYNSTM
                     VPMRGFIEALGGTVDYNDTTRAATFSFSR"
     gene            complement(1667..>1966)
                     /locus_tag="PK717_07705"
     CDS             complement(1667..>1966)
                     /locus_tag="PK717_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47580.1"
                     /translation="KTDISSDTFTLYFTLSVENLPKGVTPTYVIHLEDAPKPLEFTTS
                     RIQLNLQRVCPGNPFAEAEDYNIRIQAINSSTRQTIAEEKFKYTKCPIKVGNDAP"
BASE COUNT          554 a          462 c          472 g          478 t
ORIGIN      
        1 gaatgttcca atagcctggt ctgtagaacc agccggtctc ggaatctttg atgcaaacgg
       61 caaatttact gctggtgcca ttgcaggcac tggaactgta aaggcctgct cagccggtgt
      121 ttgtggaacc tccacgataa cagtcatggc atcaggccag attgaaacta tcactgtttc
      181 cccatccaat atcacaatga acatgggcga gacccaggca ttcaaggcaa tcgtcaaaga
      241 caagaatggc tttgaaatct ccggtgcccc gatttcatgg tcggtcaatc cgcctgacta
      301 tggcacaatc agcaccaatg gcgtgttcat tgcctcaaag ccaggcatgt gcgtaatcat
      361 cgcccagtcc ggcgacaagt ctggaacagc aatcgtcaac atcgtccagg tgacaaggat
      421 tgacctcaca ccgaagtcag taattattgc accgaataca acccagcagt tcacggccgt
      481 tgcttatgga cagactggcc agcaaatacc aggaatcaaa ttcaactgga cagtaacacc
      541 acaaacgctt ggtgcaatat cagagagcgg tttgtttact gctcttggtt caccaggaca
      601 ggcaggactc ataacagtgt ctgcggcagg aatgactgca acggcaactg ttaccatagg
      661 catcccggac aacacaccac caagaatcac acttgtgaag ccaaccataa atggtggaaa
      721 ctcaccgatt ggcccgtcaa cagttgaagt caaggttgag gacgaatcag gtgtgtctga
      781 agtgacgatc aacggtgttc cggcaaccat gaatgccaac ggcaactggg cagcaaacgt
      841 catcgtcaat ccaggcaaca acaccttcat ggtcaaggca aaagacaact caaccagccg
      901 caacgaggcc acacttgcag tcacaatcta tggcgcaact gagtctagga tacaaatgag
      961 aataccgaca aacaaccagg ccgatgtcaa aactgttacc ataactgaaa acaatgtgcc
     1021 aagaactata acactcaaga cttctccaga gctctaccag aacagcacat tcgtcggtct
     1081 tcgtgatctt gccgaaaatc tcttgggggc aactccagag aaaggcggaa gtgttgctta
     1141 cgatggtgca accagaaaaa ttacaataac catcaagaga accaatggtg atgtgatgat
     1201 attcgaaaca gttgttgggg agaaggacta ccagctccag acaatccatc ctgatggtac
     1261 aagggagatc aagaccgaag tcatggccca ggcgccatac attggatctg ccgcaacaaa
     1321 atccagaaag gtcaactaca acagcacaat ggtcccaatg aggggattca ttgaagcact
     1381 tggcggtaca gttgactaca acgatacaac aagagctgct accttcagtt tctcaaggta
     1441 atcctgttgt aaagtagtaa aaacaaaagc aaaaagcccc gggttttgcc cggggctttt
     1501 tctatttcag ggaggtattt tggggattac cagtgtctag tgaatattgc tgttcttttg
     1561 cctacctatt ctcgtgttgg catggatggt ttgttttctg tgaggtgttt tgttttgaag
     1621 tgccgaagaa cttcggcact tcaaactgca tcctgtgttt tactttttat ggggcatcgt
     1681 tacctacttt tatggggcat ttcgtatatt tgaacttctc ttccgcgatg gtttgcctgg
     1741 tcgacgagtt aattgcctgg atccttatgt tgtaatcctc ggcttctgca aaaggattgc
     1801 ccgggcaaac cctctggagg ttgagctgga ttcttgatgt tgtgaattca agcggttttg
     1861 gggcgtcctc aaggtgtatg acgtatgttg gtgtaacgcc ttttgggagg ttctcgacgg
     1921 agagcgtgaa ataaagggta aaggtgtcac ttgaaatatc tgtttt
//
LOCUS       DAOSRI010000300         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54335_length_1937_cov_3.311902, whole genome shotgun sequence.
ACCESSION   DAOSRI010000300 DAOSRI010000000
VERSION     DAOSRI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54335_length_1937_cov_3.311902"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..>633
                     /locus_tag="PK717_07710"
     CDS             311..>633
                     /locus_tag="PK717_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47581.1"
                     /translation="MKKVLAVIFVLAMVLTVIPLHGDAFAASNVDPEIISMKNDGNKV
                     IEVLVKFSAEPVVQTYTYHGAFPTTSSEIMRPGNVMTSVAMTPIRFQQSVLMPKLANI
                     KGIIKV"
     assembly_gap    634..643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            676..>1937
                     /locus_tag="PK717_07715"
     CDS             676..>1937
                     /locus_tag="PK717_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR47582.1"
                     /translation="MRLPVNKVFDVARIHGVKYVTYLPEMKVDRIRSKTFLGAERVWN
                     EVKDPKGRKVDGTGIICAVTDTGIDYTHQDFGSQKSPQGKKVVFSHDFGDGDDDCQEI
                     QPSGAQNPGTSHGTACSGIIAGDGPDNEKGMAPKALLAGYKISDSKNNGGLNSDGILK
                     AFEYLVTEKIHVSNNSYGAPGGSSYMDELENNTVLAGCAVVASQGNEGSPGPKLPIPS
                     GSTATPDNVIGVGATDDTDGVIMKIEDAPTQEMQGTLFPGEWGDTGKVFGNTGAPLEL
                     VDCGWGRPEDFEGLDLTGKAALIQRGPSPVLFGDKFGPAINFKDKNLNASKAGAKVMV
                     LYNHEPGKIGALYANSPGGPLDKNLIPSFHLSRIQGEALRKELHRDNEWELGQKDTDQ
                     KKIIISFGKPSIKANVADFTSSGPTRRL"
BASE COUNT          588 a          387 c          444 g          508 t
ORIGIN      
        1 caatgaaata tgggaatcaa tccagaatta caacaagaga tttgaaaaaa tcagcaagat
       61 tgcccagaaa aacatacaaa gcctggatga cataaaagaa gaggacttgc attagctaag
      121 cttttaaata ttccttaatc aacaactgcc ctttgcattt tgtgcaaagg gcagttgttg
      181 tatttggctt ttccaaaaca tcataaataa tttcttgtat caacatcaaa tttcaattga
      241 cgaattgacc gcgtcaaact agaatgtatg caagacttaa tacaagcctg aaatggcgtt
      301 ggaggctttg atgaagaaag ttttagcggt tatctttgtt ttggccatgg tactcactgt
      361 cattccgttg catggtgatg cctttgctgc gtccaatgtt gatccagaga tcatctccat
      421 gaagaacgac gggaacaagg tcattgaagt ccttgttaaa ttcagtgctg aacctgttgt
      481 acagacatac acgtaccatg gagcattccc gacaacatca agtgaaataa tgagaccagg
      541 aaacgtcatg acaagtgttg ccatgacccc tatcaggttc cagcagagtg ttttgatgcc
      601 gaaacttgca aacataaaag gcatcatcaa ggtnnnnnnn nnncgccact tcagtacgtt
      661 tataacggcg tatatatgag acttccggta aataaggtgt ttgatgtcgc aagaattcat
      721 ggtgtcaaat acgtaactta tctgccagaa atgaaggtag atcgtatcag gtcaaagact
      781 tttcttggtg cagaaagggt ctggaatgaa gtaaaggacc caaagggtag aaaagttgat
      841 ggaacgggaa ttatctgtgc cgtcactgat actggaattg actacacaca ccaggatttt
      901 gggagccaga agtcacccca gggcaaaaaa gtagttttca gccatgattt tggtgatggc
      961 gatgatgatt gccaagaaat tcagccaagt ggtgcccaaa acccaggaac cagccacgga
     1021 acagcatgct ccggaatcat agccggtgat ggtcctgata atgaaaaagg aatggcccca
     1081 aaagcccttc ttgctggtta caaaatatct gattcaaaaa ataatggtgg ccttaatagc
     1141 gatggtatct taaaagcatt tgagtatctt gtcaccgaaa aaatacatgt gtcaaacaat
     1201 tcttatggcg cacctggtgg ttcttcatac atggatgagc ttgagaataa taccgttttg
     1261 gcaggttgcg ctgtcgttgc ctctcagggt aatgaaggtt ctcctggtcc aaaacttccg
     1321 atcccatctg gttctacggc cacaccggac aatgttattg gtgttggtgc aaccgatgac
     1381 accgatggtg ttatcatgaa gatcgaagat gccccaacac aagaaatgca gggtacgttg
     1441 ttcccaggtg aatggggaga tactggaaaa gtgtttggca ataccggcgc accgcttgag
     1501 cttgttgatt gtggatgggg aagaccagag gattttgagg gtcttgatct gactggaaaa
     1561 gcagctctca ttcagcgtgg tccttcacca gtgcttttcg gagacaaatt tggtcctgcg
     1621 atcaacttta aagacaaaaa cctgaatgca tcaaaagctg gcgctaaggt aatggtcctt
     1681 tataatcatg aaccaggtaa aattggcgct ctttatgcta attcccctgg tggtccgctt
     1741 gacaagaatc ttatcccaag ctttcacctt tcgagaatac agggtgaggc tctaaggaaa
     1801 gagctccacc gtgataacga gtgggaactt ggtcaaaaag atactgacca gaaaaaaatc
     1861 atcattagtt ttggcaaacc ttcgataaaa gccaatgtgg ctgattttac ttcctctggt
     1921 ccaacccgca gattgat
//
LOCUS       DAOSRI010000301         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54526_length_1932_cov_3.320725, whole genome shotgun sequence.
ACCESSION   DAOSRI010000301 DAOSRI010000000
VERSION     DAOSRI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54526_length_1932_cov_3.320725"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK717_07720"
     CDS             <1..517
                     /locus_tag="PK717_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47583.1"
                     /translation="ATGGGDSVSKSVCLVSMMRLARIPARFKVSIADSSYLKGLYVGI
                     VERFVPKTLVHLMPEIHLKGRWITLEGVVLDRMYLEKLSTILNDDRPCFSLGVADKRP
                     LLVLSKDIENWDGESDTFCQSTCVLEDLGVHTNVEEIFAEHIPKKVQSFIFSTVVSPS
                     ITKNVSRLRMI"
     gene            514..1392
                     /locus_tag="PK717_07725"
     CDS             514..1392
                     /locus_tag="PK717_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47584.1"
                     /translation="MTKGFYRPQGQRSIEVVSNLGILVPTVWELFNDKAKLARAWGEG
                     LEISTIEGCEGQIWGLEPSFSASWHIETQESRQYESLKAIFEGNPWHHRPDLFELAVQ
                     FEFGEICKLKLSGFDLTHTGDAPLLSASSWAYTKMAMLTKFFGKEPEKCVSGTRSIEA
                     STIIKATPDTLRSMLLRADEAVKWLADMVQIEARESGQFQIAWQRPWGNILLHGVVSE
                     FESDRITLVAKDNPFSPASPLSLRFSFFQEGENTKLSVVFDGFDVQPWASFSVLLFSE
                     IAQVSLAGLGLIVSEQ"
     gene            complement(1405..>1932)
                     /locus_tag="PK717_07730"
     CDS             complement(1405..>1932)
                     /locus_tag="PK717_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47585.1"
                     /translation="VNIVYTYVDDQGRQKLLLDDGTKTKILWEGEYGLGVFNHIKKQA
                     SPVCFLGNGLVAAALATETGYSIAVFDTTSGQAKFFEPLKRFPTNITAIKYSGSVALK
                     MTFSNGLSKTITVLPDGSKLEIPEEIANASFYDSDGTWLDAGNGYIEHRFCEGLSAFI
                     KKFGERYVVVWAGKP"
BASE COUNT          485 a          433 c          494 g          520 t
ORIGIN      
        1 tgcgacaggt ggcggggact cggtttcaaa atccgtttgc cttgtctcca tgatgagact
       61 tgccaggata ccagcgaggt tcaaggtttc catcgccgac agctcgtacc tgaaggggct
      121 ttatgtgggt atcgtcgaga gatttgttcc gaagaccctg gtccacctca tgccagaaat
      181 ccacctgaag ggcaggtgga taacgcttga gggggttgtc ctggacagga tgtatctgga
      241 aaagctttca acaatactca atgatgacag gccatgcttc agtcttgggg ttgcagacaa
      301 gaggccgctc cttgttctct caaaagacat agagaactgg gatggcgaga gtgacacttt
      361 ctgccagtcc acatgtgttc ttgaggatct tggtgttcat actaacgtgg aagaaatttt
      421 tgctgaacac ataccaaaga aggtacagtc ctttattttt tcgacagttg tttcgccatc
      481 gatcacaaag aatgtatcga gattgagaat gatatgacaa agggcttcta caggccccag
      541 ggccagcgct caattgaagt ggtctcaaat cttggcatcc tcgtgccgac agtctgggag
      601 ttatttaatg acaaggccaa acttgccagg gcttggggtg agggcctgga aatttcaaca
      661 attgaaggct gtgaagggca aatatggggc cttgaaccaa gtttctcggc ctcgtggcat
      721 atagaaaccc aggaatccag gcagtatgag agcctgaagg caatttttga gggcaatccg
      781 tggcaccaca ggcctgacct cttcgagtta gccgtacagt tcgaatttgg ggagatatgc
      841 aagctcaaat tgtcagggtt tgatctgacg cacaccgggg acgcccccct gctttccgcc
      901 tcatcatggg cctacacaaa aatggccatg ttgacaaagt tttttggaaa agagcctgaa
      961 aaatgtgttt ctggcacaag atcaatcgag gcatcaacaa ttataaaggc gacaccagac
     1021 actctcaggt cgatgctttt gcgtgccgat gaggccgtga agtggcttgc cgacatggtc
     1081 cagattgaag ccagggagag cggccagttc cagattgcat ggcagaggcc atggggaaat
     1141 attttgcttc atggggttgt ttctgaattt gaaagcgaca ggataacgct tgttgcaaaa
     1201 gacaatcctt tctcaccagc atctccgctt tccttaaggt tctccttttt ccaagagggc
     1261 gagaacacaa aattatcggt tgtctttgat ggttttgatg tgcagccctg ggcatcattt
     1321 tcggtgcttc tattttctga gattgcccaa gtttcgcttg caggactcgg tttgattgtc
     1381 agtgaacagt agatattgat aaaatcaggg cttgcctgcc cagaccacta catatctttc
     1441 cccaaatttc ttgatgaagg ccgaaaggcc ctcgcagaac ctgtgttcga tgtatccatt
     1501 tccggcatca agccatgtcc cgtctgaatc ataaaagctg gcatttgcaa tttcttctgg
     1561 tatctcaagc ttgcttccat cgggaaggac cgtgattgtc tttgataggc cattcgaaaa
     1621 cgtcattttc agggccaccg agccagaata ctttatggcg gttatgtttg taggaaagcg
     1681 cttcaggggt tcaaagaatt ttgcctgccc ggaagtggta tcaaaaacag caattgagta
     1741 gccagtttct gtggcaagcg ctgctgcaac aaggccattt ccgagaaaac agactggtga
     1801 ggcctgtttt tttatgtggt tgaaaacacc aagcccgtat tcgccttccc agaggatttt
     1861 tgttttggtg ccatcatcaa ggagcagttt ttgccttccc tgatcgtcaa cgtaggtgta
     1921 aacaatattt ac
//
LOCUS       DAOSRI010000302         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54686_length_1928_cov_3.891084, whole genome shotgun sequence.
ACCESSION   DAOSRI010000302 DAOSRI010000000
VERSION     DAOSRI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54686_length_1928_cov_3.891084"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK717_07735"
     CDS             <1..304
                     /locus_tag="PK717_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOR47586.1"
                     /translation="LIAFFLAFALRPKRIDSDLATTSYECGIPPKTDSKIKFNIRYIV
                     FALIFVAFDVEAVFLYPWAVKVKELGTYAIFEVTLFIAILLVGYFYAWGRGLLKWD"
     gene            295..798
                     /locus_tag="PK717_07740"
     CDS             295..798
                     /locus_tag="PK717_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOR47587.1"
                     /translation="MGLTQTPAPTELEKSTVLLAPLDWLVGWARKNSLWPLTYATACC
                     AFEMMQAWMPRYDFDRHGCFFRPTPRQCDVMIVAGTVTKKMAEPTRLLYEQIPNPKWV
                     IAMGACACSGGPFIDSYSVVKGVDKIIPVDVYVPGCPPRPEALQFAILELEKKIMKER
                     ANLGRQP"
     gene            795..1226
                     /locus_tag="PK717_07745"
     CDS             795..1226
                     /locus_tag="PK717_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOR47588.1"
                     /translation="MSTSKDIAKQKFGIDLSPMPAPETALMINVDSSKAAETCLKLRE
                     SGFSILSMLTAKCKDNGFEMVYMLDNWETKERVFIFAPLLAQDPKIKSLTPDWEAADW
                     LEREVYDMFGIVFEGHPNLQRIITPENFTGHPLRKDFTDTD"
     gene            1236..>1928
                     /locus_tag="PK717_07750"
     CDS             1236..>1928
                     /locus_tag="PK717_07750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOR47589.1"
                     /translation="MEEKIRTDLLEVNFGPQHPATHGVFRAVLTLDGEIVVGCKPVLG
                     YLHRGIEKIAERLTYQQFIPYLDRLDYISGFLNEYPYVKAVEKLMGIEVPIRAQYIRV
                     LMMELNRIAAHLVNVGAQGLDLASMTPMVYCLREREYILELMAEVSGSRMTFNYFRFG
                     GVKFDLPEGYLSKLDRFIHYFKDRLPEYASMLHKNEIFQARTKNIGILKPETALKLSC
                     MGPTLRGCGIARD"
BASE COUNT          555 a          470 c          452 g          451 t
ORIGIN      
        1 tcttatagca ttcttcctcg cctttgcatt aagaccaaag agaatcgata gtgacctcgc
       61 aacaacgtcc tacgagtgcg gaataccgcc aaagacagat tcaaaaataa aattcaacat
      121 caggtatatc gtttttgcat tgatattcgt cgcatttgat gtggaagctg tttttctcta
      181 cccatgggcc gtaaaagtaa aagaactcgg aacttacgcc atttttgaag taacactctt
      241 catagcgata ctcctcgttg gatatttcta tgcttgggga agggggctgt tgaaatggga
      301 ttgacacaaa cacctgcacc aacagaactt gaaaaatcaa ctgttcttct tgcaccactg
      361 gactggctgg tgggctgggc cagaaagaat tccctttggc cactcactta cgcaacagcc
      421 tgctgcgcat tcgaaatgat gcaggcatgg atgccaagat acgacttcga caggcatggc
      481 tgcttcttca gaccaactcc aagacagtgc gatgtcatga tcgttgcagg aactgtaaca
      541 aaaaagatgg cagaacctac aagactcctt tatgagcaga tccccaaccc gaagtgggtc
      601 attgcaatgg gtgcctgcgc ctgttctggc gggcctttca ttgacagtta ttccgtagtt
      661 aaaggcgtcg acaagataat tcctgtcgat gtgtatgtcc cagggtgtcc accaaggcca
      721 gaggccctcc agtttgccat tcttgagctt gagaaaaaaa tcatgaaaga aagagccaat
      781 cttgggaggc aaccatgagc acctccaaag acattgcaaa gcaaaaattt ggtattgacc
      841 tctctccaat gcctgcccca gaaacagccc tgatgatcaa tgtagacagc tcaaaggcgg
      901 ccgagacatg cctgaagctc cgcgaatctg gtttcagtat cctctcgatg ttgaccgcaa
      961 aatgcaaaga caatggtttt gaaatggtct acatgctgga caactgggaa accaaggaaa
     1021 gggtctttat tttcgctcca ctgcttgcac aggacccaaa aatcaagagc ctgacgcctg
     1081 actgggaagc ggcagattgg ctggaacgcg aagtctatga catgtttgga atagtgtttg
     1141 agggccatcc aaacctccaa agaataataa caccggagaa tttcacaggg catcctctcc
     1201 gcaaagattt cacagatact gactgaggag ggaaaatgga agaaaaaatc agaactgacc
     1261 tgcttgaagt gaactttggc ccccagcacc ctgccactca cggtgttttt agagcagtcc
     1321 tcacccttga cggtgaaatt gtggtcgggt gcaaaccagt gcttggctat ctccacaggg
     1381 gaatagaaaa gattgccgaa agactgactt accaacaatt tattccttat ctagacaggc
     1441 ttgattacat ctctggtttc ctcaatgagt atccctatgt aaaggccgtt gaaaaactca
     1501 tggggatcga ggtgccaatc agggcacaat acataagagt cctgatgatg gaacttaacc
     1561 gcattgcggc gcaccttgta aacgttggcg cgcagggact cgacctggca tcgatgaccc
     1621 cgatggtcta ctgcctcagg gaaagggagt acatcctgga gctgatggca gaggtttccg
     1681 gatcaaggat gaccttcaac tacttcaggt tcggcggcgt caagttcgac cttccagaag
     1741 gatacctctc aaaactcgac aggttcattc actacttcaa agacagactc ccagaatacg
     1801 cttcaatgct ccataaaaac gagatattcc aggcaaggac aaaaaacata ggaatcctga
     1861 aacccgaaac tgccctcaag ctctcctgca tggggcctac cctcaggggt tgtggcattg
     1921 caagggac
//
LOCUS       DAOSRI010000303         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54735_length_1927_cov_2.946581, whole genome shotgun sequence.
ACCESSION   DAOSRI010000303 DAOSRI010000000
VERSION     DAOSRI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54735_length_1927_cov_2.946581"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PK717_07755"
     CDS             complement(<1..1715)
                     /locus_tag="PK717_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR47590.1"
                     /translation="MRIERVWFGKRRTEVNPPDLIRIQTDSYKRFLTEDLKNLIASLN
                     EDRSLRESGKVEVEFVDYTLGDPPYSQIECKQRSLTYTIPLKLTVRLINKETGEIKEQ
                     ELFAGELPQMTDAGTFIINGVERVVVSQLVRSPGVYFEFEQLPNSNRNRAMATVIPDR
                     GSWLEFEMGDDDICYVKIDKKSKKFPVTELLRVMGDYTDDEIRNLFNQDKTSEAKSDN
                     KTDLERAKGKPLADTIYHPTTGDDLYQAGTTLTNIILDEIKRLGIKKVKYFDKDIASF
                     IVATLEKDQTKTREEALVDMFKHIRPGERPTIENAESLIHNLLRDPRRNNLSVVGRYK
                     INKKLELNLDSNLVTLEDVRAILKFLFQLKLGNVPPDNKDHLANKRIRAVGELLENQL
                     RVGFLRMLKIVREKIITLPDEELTVQNIINVRPIIAAMREFFGLGQLSQFMDQTNPVA
                     ALTHKRKLSSLGPGGLNRDRAGADVRDVHHSHYSRICPIETPEGGKIGLINSLTSHSI
                     VDEFGFIRAPYFKVIEGRVTDEVIYLTADDEENYNIAQANTETDRKGIIKGTAICRQK
                     GGEIV"
BASE COUNT          448 a          421 c          436 g          622 t
ORIGIN      
        1 tctacaattt ctccaccctt ttgcctgcag atggcagtgc ctttgattat tcctttgcgg
       61 tcagtctcgg tgtttgcctg tgcgatattg tagttttcct catcatcggc agtcaggtag
      121 atgacctcgt ctgtgaccct tccctctata accttgaaat agggggccct tataaagcca
      181 aattcatcga cgattgagtg cgaggtcagt gagttgataa gaccgatttt tccgccttca
      241 ggtgtttcaa tagggcatat ccttgagtaa tggctgtggt ggacatcacg cacatctgca
      301 ccagcacgat ccctattgag accacctggt ccaagagatg aaagtttacg tttgtgggtg
      361 agtgcagcga caggatttgt ctggtccatg aactgggaaa gctggccgag gccgaagaat
      421 tccctcatgg ctgcaattat tggcctaacg ttgatgatat tctggacggt aagctcttca
      481 tccggaaggg tgatgatctt ttcccttaca atcttgagca tcctgagaaa gccgaccctg
      541 agctggtttt cgagcaattc accaactgcc ctgatacgct tgtttgcaag gtggtccttg
      601 ttgtctggtg ggacattgcc cagttttagc tggaacaaga atttcaggat tgctcttacg
      661 tcttcaagcg taacgaggtt ggagtcaagg tttagctcga gcttcttgtt gattttgtat
      721 ctaccaacaa ccgagaggtt gtttcttctt ggatccctga gcaggttgtg aataaggctt
      781 tcggcatttt cgattgttgg acgctcacca ggcctgatgt gcttgaacat atcgacaaga
      841 gcttcttctc ttgttttggt ctggtctttt tcgagtgtgg ccacaatgaa gctggctatg
      901 tctttgtcaa agtattttac ttttttgatt ccaaggcgtt ttatttcatc gaggattatg
      961 ttagtgagtg tcgtaccagc ttgataaaga tcatcaccgg ttgttgggtg ataaatagta
     1021 tcagcaagag gttttccttt tgccctctcg agatcagttt tattgtcaga ctttgcttcg
     1081 gaggttttgt cctggttgaa gagattacgg atttcatcat ccgtgtaatc gcccatcacc
     1141 ctcagtaatt ctgtgacggg gaatttcttg gattttttat ctatcttgac ataacaaatg
     1201 tcatcatcgc ccatttcaaa ttcgagccat gaacctctgt ctggtatgac ggttgccatg
     1261 gccctgtttc tgtttgaatt tggcagttgt tcaaattcga aatacactcc tggtgagcgc
     1321 acgagctggg aaacaacgac acgctcaacg ccgttgatta tgaatgttcc ggcatctgtc
     1381 atttgtggaa gctctccagc gaaaagctcc tgctctttta tttcgccggt ttctttattt
     1441 ataagcctaa cagtgagttt taatggtatg gtgtatgtca aactgcgctg tttgcattca
     1501 atttgactgt atggtggatc accgagtgtg taatcaacaa actcgacttc gacttttccc
     1561 gattcacgaa gtgaacgatc ttcgttcaat gaagcaatga ggtttttgag gtcctcggtc
     1621 aaaaaccttt tgtaagaatc ggtctggatt cttatcaaat ctggtggatt gacctcagtt
     1681 cttctcttgc caaaccaaac ccgttcaatt ctcatgtggt ttccttccta ccccactcat
     1741 cagggcatac gaattgctag gggcatattc acaaaagaat atcctaacct atggcccttt
     1801 taatgtcaat gaacaatggt taaggtaacc acactctttc aattactata aaacctttga
     1861 gcaggtcagt atttagtgtg cattcaagca cagacctgag aatttcttgc gtcagataga
     1921 acgaacc
//
LOCUS       DAOSRI010000304         1923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54866_length_1923_cov_4.273555, whole genome shotgun sequence.
ACCESSION   DAOSRI010000304 DAOSRI010000000
VERSION     DAOSRI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54866_length_1923_cov_4.273555"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..926
                     /locus_tag="PK717_07760"
     CDS             3..926
                     /locus_tag="PK717_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR47591.1"
                     /translation="MAYNFRCNFKCAHCATTRMIKKDRKIDPGILLDLSNQAEALGLC
                     QFVISGGEPLLYPDLEEIFKALQPSKFHIAMSTNGWFMTEEKAIWLKKLGLDKVKISI
                     DDFDEKIHDENRGVEGAYKRALKAMEFCKKAGMGVAIQAVVTHQNCKTDRTIKLAEFA
                     QNMGYDLDIIVARAIGRWEGRHEVLIDQEDADFLWEVHQKYPALHRDTFPTYGMHRGC
                     GCVDSILHVTPYGDVLPCVFIHIAIGNIFEEPLKDIIHRGQHLKPFACFSKLCLSGED
                     RDFINQYMTKFYGKPIPIPWQEAFTEADIIL"
     gene            923..>1923
                     /locus_tag="PK717_07765"
     CDS             923..>1923
                     /locus_tag="PK717_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOR47592.1"
                     /translation="MIRYTELKNTNRHNLMEVLPLAKPFTLLVEPSSLCNFHCIHCFQ
                     SLKQDNYFTRNRKNMPLGRFQRIIEQMQAWPGIKLKVLKLSLYGEPLLNPDFGAMLRL
                     AKEAAIADRIETTTNASLLTPEVARNMVDCQLDYLRVSIYATDATRHREITGSGIAPE
                     VIHENLRVLQEIKRQRGSERPFVSPKMLDTYDQTQNDAFKAMYQDVADELYLDKPHNW
                     IQTGEQKFTEKIYQADHSKAVADFQAHSTRRIACPMPFTTMTVRCNGDVSPCCVDFIG
                     GTNLGNMEEKPLQDLWTSPEWLAFQIMQLENRKTENFSCARCDVYLSDHYTRDNIDG"
BASE COUNT          545 a          411 c          459 g          508 t
ORIGIN      
        1 atattgcata taattttcgg tgtaatttta aatgtgcgca ttgcgcaaca actcgcatga
       61 tcaaaaaaga tcgaaaaatt gatccaggga tacttcttga tttaagtaac caggcagaag
      121 ctttaggact atgccaattt gtgatatctg gtggtgagcc gttgttgtat ccagatttgg
      181 aagaaatttt taaggcattg cagccaagca agtttcatat tgccatgagt acaaatggct
      241 ggtttatgac agaggaaaaa gctatctggc ttaaaaaatt aggattagac aaagtcaaga
      301 ttagcattga tgattttgat gaaaaaatcc atgacgaaaa ccgtggagta gaaggtgcat
      361 ataagcgagc attaaaagca atggaatttt gcaaaaaggc aggtatggga gtagctatcc
      421 aggcagtagt cacccatcaa aattgcaaaa cagatagaac aattaaatta gcagagttcg
      481 cgcaaaacat gggctatgat cttgatatca tcgttgcccg agcgattggc aggtgggaag
      541 ggcgccatga agtcctaata gaccaggagg atgctgattt tctctgggaa gttcatcaga
      601 agtatccggc gcttcaccgg gatactttcc ctacttatgg aatgcacagg ggatgtggat
      661 gtgttgattc tattctgcat gtgacgccct atggcgatgt attgccctgt gtttttattc
      721 atatcgcaat tggtaatatt tttgaagaac ccctcaaaga tatcattcac cgtggtcaac
      781 atttaaagcc ttttgcttgc tttagcaagt tgtgtctttc aggggaagac cgtgatttta
      841 ttaatcaata tatgactaaa ttttacggga agcctattcc aattccctgg caagaagctt
      901 ttacagaagc ggatattatt ttatgattag atatactgag ttaaaaaaca ccaatcgaca
      961 taatttgatg gaggtcttgc cgctggcaaa gcctttcacg ttgctggttg agccctccag
     1021 cctctgcaat ttccattgca tccattgttt tcagagcctg aaacaggaca attatttcac
     1081 ccggaaccgt aaaaatatgc ctctgggccg ttttcagagg attatagaac agatgcaagc
     1141 ctggccgggc ataaagctga aagtcctgaa attgagcttg tacggggagc cattgctcaa
     1201 tccggatttt ggcgcgatgc tgcgcttagc taaggaagca gctatcgccg accgcatcga
     1261 aactacgact aacgctagtc tgttgactcc tgaggtcgcc cggaacatgg tggattgcca
     1321 gttggattac ctgcgcgtat ctatttatgc gaccgatgcc acccgacatc gggaaatcac
     1381 cggctctggc atcgccccag aggtcattca cgaaaatctg cgggtcttgc aggaaatcaa
     1441 gcggcaacgg ggttccgagc gcccatttgt cagtccgaaa atgcttgata cgtacgacca
     1501 gacccaaaat gacgctttca aggctatgta ccaagacgta gccgatgaac tttacctgga
     1561 caagccgcat aactggattc aaacgggtga acagaagttc acggaaaaaa tctaccaggc
     1621 ggaccacagt aaggctgtag cggatttcca ggctcatagt acgcgaagga tcgcttgccc
     1681 gatgcccttt accacgatga cggttcgctg caacggcgat gtctcgccct gctgcgttga
     1741 ctttatcggc gggactaatc ttggcaacat ggaggagaaa cctctgcaag acttgtggac
     1801 ttccccggaa tggcttgctt ttcaaattat gcagctggaa aaccgaaaaa ccgaaaactt
     1861 ctcctgtgcc cgttgcgatg tttatctgag tgaccactac actcgcgata atattgacgg
     1921 ctt
//
LOCUS       DAOSRI010000305         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_54961_length_1921_cov_3.946945, whole genome shotgun sequence.
ACCESSION   DAOSRI010000305 DAOSRI010000000
VERSION     DAOSRI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54961_length_1921_cov_3.946945"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="PK717_07770"
     CDS             <1..1753
                     /locus_tag="PK717_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR47593.1"
                     /translation="DVSAPGVGIETTNPTWDAKNPYMFTFGGTSAAGPFVAGCVSLIR
                     QARPDWNPFEIKRAVMNTAIPLKRFDGQGYIPLTVQGQGRINVYNAINTDLMFQPPSG
                     LIDTNNNNAIHVADWPAEWADPDKRSQLPANVRDYPIGMKIYNYNDKENKNIEFSFEV
                     NARYPERIDVSFSTKNLTVPKMVGKKPGEAWVGFNITLPKNIQGNVNDIFIWATDKAT
                     GKKWHVGWCIYNNQNPRNNTHAGAIEINPPEFTPNGDGEQDEIKVDYTLTNGTLQLWS
                     GYAWDSYLNFQDELIFWAIDLNSERWVKIHEASNVEIGNHSFIWDGKDSFGNYILPEG
                     EWFIQVSASGSKLDLSKRAFVPISDDFNLLNESFVVSKSTVPPLPTIFAYALPIEPGV
                     GDEFSVGIYIKYAVNVKTLQFQINLKGSEGIIKYLGHEVGEFMQRDEPNLLTNIEYDE
                     EKNIVNVDMQRALDGVSGEGYLLKLRFLAEEANYFDVNFQNLLVTRLEDPSDPTSEKS
                     AKAFYRKGEISIYKTAYSVADFNRDGVVNEKDAAIILGKLGLRRGDDGFFWRCDLNFD
                     DVIDINDLTEFAKLYQN"
BASE COUNT          602 a          361 c          426 g          532 t
ORIGIN      
        1 agatgtttca gctcctggag ttggcataga aaccacaaac ccaacatggg acgcaaagaa
       61 cccatatatg ttcacatttg gtgggacctc tgctgctggc ccatttgtgg ctggttgcgt
      121 ctctcttata cgtcaggcaa gaccagactg gaatccattt gaaataaagc gtgccgtaat
      181 gaacacagca atcccgctca agcgttttga tgggcaggga tatattcctt tgactgttca
      241 gggacaggga agaataaatg tttacaatgc gataaataca gatcttatgt tccagccacc
      301 atcaggtctt attgatacaa acaataacaa tgccattcat gttgcagatt ggccagcaga
      361 atgggcagat ccagacaaga gaagtcagct tccggccaat gtcagggact atccgatagg
      421 aatgaagatt tacaattaca acgacaaaga gaacaaaaat atagaatttt cctttgaagt
      481 taatgcaagg tatcctgaaa gaattgatgt atcattctct acaaaaaatc ttactgttcc
      541 aaagatggtt ggtaaaaaac ctggagaagc atgggtagga ttcaacatta cactcccaaa
      601 aaacattcag ggtaacgtta atgatatttt tatttgggcg accgataagg caactggcaa
      661 aaagtggcat gttggttggt gtatttataa caaccagaat ccaagaaaca acactcatgc
      721 tggagcaata gagatcaatc ctcctgaatt tactccaaat ggtgatggtg agcaggatga
      781 aattaaagtc gactacactt taaccaatgg taccctacag ctttggagtg gttatgcttg
      841 ggattcttat ctcaacttcc aggatgagct gattttctgg gcaatcgatc taaattcaga
      901 aagatgggtg aagattcacg aggcttcaaa tgtggaaatt ggcaaccata gcttcatctg
      961 ggatggcaaa gatagctttg gaaactatat tcttcctgaa ggagaatggt ttatccaggt
     1021 ttctgcttca ggatcgaaac ttgatctgtc taaacgagct tttgtaccta taagcgatga
     1081 ttttaatctc ctgaatgaat cttttgttgt ttcaaaaagc actgtgccgc cgctacccac
     1141 gatatttgct tatgccttgc cgattgagcc gggtgttggc gatgaattct ctgttgggat
     1201 ctatatcaaa tatgccgtca atgtaaaaac attacaattc cagatcaatt tgaaaggttc
     1261 agagggaata ataaaatatc tcggtcatga agttggtgaa ttcatgcaga gagatgagcc
     1321 aaaccttctg accaatatcg aatatgatga agaaaaaaat atcgtgaatg ttgacatgca
     1381 gagagccctt gatggagtca gtggagaagg ttacctcctc aagctcaggt tccttgccga
     1441 ggaagcaaac tattttgatg tcaacttcca gaacctctta gtaacaagac ttgaagatcc
     1501 atcagatcca accagcgaaa agagcgccaa ggctttctac aggaaaggtg aaatttccat
     1561 ctataaaaca gcatattctg ttgccgattt caacagagat ggtgtagtca acgaaaaaga
     1621 tgctgcaata atccttggta aactcggcct gaggagaggt gatgatggct tcttctggag
     1681 atgcgatctc aactttgatg atgttattga catcaatgat ctcacggaat ttgcaaaact
     1741 gtatcagaat tgatcaggta agaaacaaaa ggggcggtga ttttaccgcc ccttttttca
     1801 tattatgaaa acaagggctt tcgaacaaaa ttttttgggt tttgcagctt caaagaaact
     1861 ccttgcaaaa ggagataaaa ttcttgtggg ttgttctggg gggcctgatt cagtttgtct
     1921 t
//
LOCUS       DAOSRI010000306         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55111_length_1917_cov_4.620838, whole genome shotgun sequence.
ACCESSION   DAOSRI010000306 DAOSRI010000000
VERSION     DAOSRI010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55111_length_1917_cov_4.620838"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..857
                     /locus_tag="PK717_07775"
     CDS             213..857
                     /locus_tag="PK717_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOR47594.1"
                     /translation="MCMLFVEPSSKRTIAFFDGQNLFYAAKKAFGYSWPNFDPLKLAE
                     AVCCNQDWRLIETRFYTGVPSPEDDASWSHFWMAKLANMGRSGIKTFSRQLKYRNQTI
                     SLSDGSKTAILVGNEKGIDVRLALDVVSAIRKNICDIALIFSQDQDLSEVAEEAREIA
                     RQQGRWFKMACAFPISPTYNNRRGIDKTDWVRFDRKVYDSCLDPRDYRPKDQKR"
     gene            complement(1129..1522)
                     /locus_tag="PK717_07780"
                     /pseudo
     CDS             complement(1129..1522)
                     /locus_tag="PK717_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785204.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          479 a          430 c          472 g          536 t
ORIGIN      
        1 aatatttaat attctctgtc agtttgcatg tgactttata cctcttgtgc cagatgggga
       61 gtactcacct agatttttgg tgaggcaact cggaatcttg acttttgggt ggatgatcgg
      121 gataatactt ttgtaacagg tagcaggttt gatttactcc ccgctggaca cgccgaaagg
      181 cctccggcgg ggttattttt ttggcggagg tcatgtgcat gttatttgtt gaaccttcat
      241 cgaaacgtac aattgccttt ttcgatggac agaacctttt ttatgctgcc aagaaagctt
      301 tcgggtattc ctggcccaac ttcgatcccc tcaagcttgc tgaggcagtc tgctgtaatc
      361 aagactggcg actgattgaa acgcgttttt acaccggagt gccaagcccc gaggatgatg
      421 cctcctggag tcacttctgg atggccaaac tggcaaatat gggtcgcagc ggcatcaaga
      481 cgttttccag gcaactcaag taccgcaacc agaccattag tctttcagac ggaagtaaaa
      541 ctgcaatcct ggtcggtaac gaaaaaggca tcgatgtccg acttgccctt gacgttgtgt
      601 cagccataag gaaaaacata tgcgacatag ccctgatctt cagtcaagat caggatcttt
      661 cagaggttgc agaagaggct cgggagattg caaggcaaca ggggcgctgg ttcaagatgg
      721 cttgtgcctt cccaatcagt cccacttaca acaacaggcg tggaatcgac aagactgact
      781 gggtccgatt cgatcgaaaa gtttacgatt cctgtctcga tccgcgagat tatagaccca
      841 aagaccagaa gaggtagcat ttttcaggag atacaggttc aagagattgc ttcggtgaag
      901 gccatgatca aattggttac tatcacccaa acccaggatt tccccctccc cccagtagaa
      961 aacctgaatt gagaaatctt ccgggctctg ctgtcaagta agacattcag atttgttaat
     1021 tatggaaagc agatagatgg gtcatttcca ggcgtctcac tgtctgaatg ggagggttca
     1081 ttacagccaa tattggttga gttattgtca aacctggtgt gcggggtctc atgcggcgtt
     1141 cctgtcggtt gcccgttgcc cgtacactgt ctttgaaaag gacgccttcg atcacttcgc
     1201 cgataagatc gaaacctttg atacgcttca acttgttttc acctgactgg atcagcttga
     1261 agaccatagc cagtgtcgtt tctctcgatc cgcagcttct tgtctttgcg gttctcagcc
     1321 ttaccgttga aaatgtagat tctatggggt tcgtggttct tatgtggatc cagtgcactg
     1381 cagggaagtc atagaatgag agaagatctt ccctgtcttt tctcaggcag tcggttgctt
     1441 tagggtattt gtcgccgcaa cgttccagga atctgtcgaa ggctttttca gcctcttctt
     1501 tcgtctccgc catccagatt tgagcttaac tcagcggatc gcggttatat gcagaaagat
     1561 gtctcttttg aggactgggc atttttgaac catctttcct tgatgatgat atatcggctt
     1621 ttcgtcagga tgaaggaagc ggacatgctg agcaaatatt ctgtaaagga ttgtcttttc
     1681 ttcttgtccg ggataaggaa gcagcagagg ggaaaaactg ggagactact ttgacgacca
     1741 agaaaactga aaaaatactg agcctgctca gctgtgatat caaaatagac tcaggtatat
     1801 agtacccatc gttggaaaag tttggacctt aaacatgtca taaagccttc caattactga
     1861 agaactggcg gagggcagag gattcgaacc cctggagact tgcgcccctg ctgattt
//
LOCUS       DAOSRI010000307         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55172_length_1916_cov_2.793659, whole genome shotgun sequence.
ACCESSION   DAOSRI010000307 DAOSRI010000000
VERSION     DAOSRI010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55172_length_1916_cov_2.793659"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK717_07785"
     CDS             complement(<1..570)
                     /locus_tag="PK717_07785"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOR47595.1"
                     /translation="MNCPPILAVKKRGPLDESIFRGHIVVADGFGNIAQSFGNPYYIT
                     YFRSSAKPFQAMTLILTGTYEYFGLELEHLAIACGSHNGQAEHTKAVLEILSKASLSE
                     RDLLCGTHEPISAAAKAELNGKPLSQLNHNCSGKHSAMLAVCKKMGWDTKTYLEPTHP
                     IQKLSLEIVAGMCGLPVSDVVVAIDGCGVQ"
     gene            644..1780
                     /locus_tag="PK717_07790"
     CDS             644..1780
                     /locus_tag="PK717_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47596.1"
                     /translation="MKRQYWTDPERLDFEVDLIPIGGNEFIMEPVVFHPDEGGQPPDR
                     GTVDDANVLDVQFKNGKVCVKLDKDLAPGKHMAKVDSHTRLENSRQHTAQHIISAIAE
                     REYKAPTHGFHIGDATSTLDLKNPINLIQAEDIEMQANRIVMMDIQVTTTFGAPSDGT
                     RVREDLLGENKDDLRIVSIGDIDTSACCGTHCVSTGKVGPIKIISIEGHKGGSRITYL
                     AGERCIAYLQKQDRIIRQLKEISTTGTEELPQAFQKYIDQTQSFQKELNRLWEVLLPT
                     EAEKAEKHQTRFGELAVLKTDTPSKLLGKLSAIIANNTKLPVVVLTSSQPQQISIVSQ
                     NGNAKEIIAAVMASFDGKGGGSPNSASGQTGKLADTDGIIKCLS"
BASE COUNT          581 a          428 c          435 g          472 t
ORIGIN      
        1 ttggacacca caaccatcta ttgccacaac gacatctgaa actggcaaac cacacatgcc
       61 ggccacaatt tcaagggaca atttctggat ggggtgggtc ggctcaaggt aggtttttgt
      121 gtcccaaccc atttttttgc ataccgccag cattgcggaa tgcttgccgg aacaattgtg
      181 atttaattgg gagagtggtt taccattcag ctctgctttt gctgcagcag agattggctc
      241 atgagttcca cacaggaggt ctctttctga caagctagct ttcgaaagta tctcaaggac
      301 agctttggta tgttcggctt gtccgttgtg ggaaccacac gctatggcca aatgttctag
      361 ttctagtcca aaatattcat aagtcccagt taaaatgagt gtcattgcct gaaagggttt
      421 ggccgagctc ctgaaatagg tgatgtaata gggattacca aagctctggg ctatattgcc
      481 aaagccatcc gcaacaacaa tgtgccccct gaagattgat tcatccagag gccctctttt
      541 tttgacggca agtatgggtg gacagttcat gatgtagagt ctatatcacg ccataacagc
      601 ttcaagattg accgttttct tttgctttcc tatactttta cacatgaaga gacaatattg
      661 gactgatccg gaacggcttg attttgaggt cgaccttatc ccaataggtg ggaatgaatt
      721 tattatggaa ccagttgtgt tccatccaga cgagggaggg caacccccag atcgtggaac
      781 tgtcgatgat gcaaacgttc tggatgttca gttcaaaaat ggaaaggttt gcgtaaaact
      841 ggacaaagac ctcgcacctg gaaaacacat ggcaaaagtt gactcccata caaggttaga
      901 gaacagcaga caacacaccg cccagcacat aatctcggca attgcagaga gagaatacaa
      961 ggctcccacc catgggttcc atattggaga tgccacatca actctcgacc tgaaaaatcc
     1021 aataaatctc attcaggccg aagatattga gatgcaggca aacaggattg tcatgatgga
     1081 catccaagtc acaacgactt ttggtgctcc aagtgatgga acaagggtaa gggaagacct
     1141 gctgggtgaa aacaaggacg atttgaggat tgtgagcata ggagatattg acacatctgc
     1201 ctgctgtggg acccattgcg tctcaaccgg aaaggttggc ccgataaaaa tcatctctat
     1261 tgaaggccac aaaggtggct ccagaataac ctatcttgcc ggagagagat gtatcgccta
     1321 cctacagaaa caggacagaa taatcagaca actgaaagaa atatccacaa caggtactga
     1381 ggaattgccc caggcgttcc agaaatatat cgaccagacc caatcatttc aaaaagaatt
     1441 aaacaggctc tgggaagttc tgctcccaac cgaagccgaa aaagcagaaa aacatcaaac
     1501 aagatttggc gagctggctg tactgaaaac tgacacacca tcaaagctcc tgggcaagct
     1561 gtctgcaatc attgccaaca atacgaaact cccggtggtt gttcttacca gttcccaacc
     1621 gcagcagata agcattgtct cacaaaatgg gaatgcaaaa gagataattg ctgcagttat
     1681 ggcaagtttt gatggcaagg gtggaggttc tccaaactcg gcttccgggc aaacaggcaa
     1741 gctggctgat acagatggaa tcatcaaatg tctgtcttaa taccaaatta aactaggaat
     1801 atggatagtt ttggcatttg attttttttg gcaatcaaaa ccgtccatct tcaccagagg
     1861 gcttcgaaaa ttctaattat ttctttgata ggcatcacct ctatcaaaaa gcacca
//
LOCUS       DAOSRI010000308         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55639_length_1905_cov_2.490811, whole genome shotgun sequence.
ACCESSION   DAOSRI010000308 DAOSRI010000000
VERSION     DAOSRI010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55639_length_1905_cov_2.490811"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PK717_07795"
     CDS             <1..791
                     /locus_tag="PK717_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR47597.1"
                     /translation="EIDYIVESDSTPALLNPPTLTETDEKIGKYIAELIKDGDCLQLG
                     IGAVPDAILNFLTDKKNLGIHSEMFSDGVVTLVEKGVINCSKKNFHPGKMVVTFLMGS
                     ERLYKFANNNPMIEMYPVDYVNNPSVIAQNDNMVSVNSALQVDFTGQVVSDTIGYKQY
                     SGTGGQLDFVRGASWSKNGRSILAFSSTAAGGKLSRIVAHIDEGASVTTPRADTHFIV
                     TEYGVADLRGKSVVERGKSLIKIAHPDFREELSRQFFEIYKRAI"
     gene            842..>1905
                     /locus_tag="PK717_07800"
     CDS             842..>1905
                     /locus_tag="PK717_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47598.1"
                     /translation="MGIRREREYGGIQPGIPTGPLTLRLPFIHYRFEWADYLQGLLMC
                     TVCLSIIPVLTGKLGMSFEVALAIVILNGTLYLAHVTFGDPVVPGWVTPAIPLLVAYC
                     ETFAPGVERMQALIAFEMLFGIFCIFLGITGLARRFIMLIPNAVQSAILVGAGFAAII
                     MIFTPGKGLKSFDSYPLTIIICMGLAFYLLFSQSFKVFKKKHRWAYYLGNLGMMPALA
                     LAIFVGPMTGEIPWPDYSSIHGIFSNPDFGTLFRDFTMLGAIPFPSAHMFISSIPMVV
                     SAYIVVFGDVIQSQALLDDAQKYRPDEQVNYNPNRSHIIFGGRNLIMSILGPDISMCG
                     PLWAAMQVVVAERYKNGPKA"
BASE COUNT          546 a          380 c          407 g          572 t
ORIGIN      
        1 ctgagatcga ttacatagtt gaaagcgatt caaccccagc tcttcttaat cctccaactc
       61 taacagaaac tgacgagaag attgggaaat acattgcaga gctcataaaa gatggggatt
      121 gcctacagct tggaattggt gctgttcctg atgcaatact taactttctc acagacaaaa
      181 aaaatctggg aattcacagc gagatgtttt ctgacggagt tgtgacactg gtagaaaagg
      241 gcgtaatcaa ctgctcaaag aaaaactttc atcctggtaa aatggttgta acgttcctca
      301 tgggaagtga aagactttat aaatttgcaa acaacaatcc catgatagag atgtatcctg
      361 ttgattatgt taacaatcca tcagttatag ctcaaaacga taacatggtt tctgtaaact
      421 cagcattaca ggtggatttt acagggcagg ttgtatctga cactattggc tataagcaat
      481 acagtggaac aggtgggcag cttgactttg tccgaggtgc ctcctggtca aaaaatggga
      541 gatccatcct tgctttcagt tctactgcag caggaggaaa actttcaaga atagttgcac
      601 acattgatga aggagcatct gttactacac caagagcaga cactcacttc atagtaacag
      661 aatacggagt tgctgatttg cgtggcaagt cagttgtaga gcgaggaaaa tccctcataa
      721 agatcgctca ccctgatttc agagaggagc tttcaaggca gttctttgaa atctacaaaa
      781 gagcgatctg attttaacag gggatttatt atattaaaat aaataaatta aggagttcta
      841 tatgggtatc agaagagaaa gggagtacgg cggaatccag cctggcattc ctacggggcc
      901 gctgacacta aggcttccgt ttattcacta cagatttgag tgggccgatt atcttcaagg
      961 tctcctcatg tgcactgtgt gtctttccat tataccagtg cttacaggta aacttggaat
     1021 gtcctttgag gttgctcttg caatagtaat tctcaatgga acattatatc tggcacatgt
     1081 tacctttggt gatccggtag ttccgggatg ggtaactcct gccattcctt tgcttgttgc
     1141 atactgcgaa acctttgcac ctggtgtaga gcgcatgcag gcattaatag cctttgaaat
     1201 gctttttggt attttctgca tattccttgg cataacagga cttgccagaa ggtttattat
     1261 gctcatacca aatgctgttc aatcagcaat tcttgttgga gcaggttttg cagcaataat
     1321 aatgatattt acacctggaa agggcttaaa aagctttgac tcatatcctc ttacaataat
     1381 tatatgtatg ggtcttgctt tctatctcct tttctcacag agctttaagg tttttaagaa
     1441 aaaacatcgc tgggcatact acctcggcaa ccttggcatg atgccagctc ttgctctggc
     1501 catatttgta ggccctatga ccggtgaaat tccatggcca gattacagct caattcatgg
     1561 aattttttct aatccagatt ttggaactct cttcagagac ttcacaatgc ttggtgcaat
     1621 accattccca agtgctcata tgttcatatc atcaattcct atggttgtaa gtgcatacat
     1681 agttgttttt ggtgatgtta tacagagcca ggctcttctt gatgatgctc aaaaatatag
     1741 acctgatgaa caggttaact ataatccaaa cagatctcac attatctttg gcggaagaaa
     1801 cctcatcatg agcatcttgg gaccagatat ctccatgtgc ggacctctgt gggctgctat
     1861 gcaggttgtt gttgcagagc gttataaaaa tggtccaaag gctat
//
LOCUS       DAOSRI010000309         1902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55746_length_1902_cov_3.373579, whole genome shotgun sequence.
ACCESSION   DAOSRI010000309 DAOSRI010000000
VERSION     DAOSRI010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55746_length_1902_cov_3.373579"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK717_07805"
     CDS             <1..363
                     /locus_tag="PK717_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR47599.1"
                     /translation="TEECDASDSFKETYLASKKEDIVIIKSPVGMPGRAISNSFIEKV
                     GKGQCMPYKCPYHCIVTCDYQRSPYCIGLALMNAKKGQMEHGFAFAGSNAYRADKVTS
                     VKEVMDSMLQEYEDSAKQ"
     gene            complement(403..744)
                     /locus_tag="PK717_07810"
     CDS             complement(403..744)
                     /locus_tag="PK717_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47600.1"
                     /translation="MGFLIGCFLASRAIEKTGDTAESARDSLKKGLLTSLFTMVQMIF
                     IWWPYFLKFFEKGFDFKNTGIPNILYTPKASLVGWLILMVIISPFLQMLMSAFGSMLV
                     LAKTKHGPKIS"
     gene            complement(922..>1902)
                     /locus_tag="PK717_07815"
     CDS             complement(922..>1902)
                     /locus_tag="PK717_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR47601.1"
                     /translation="KLIIATGSKPACIPCAKIDGKYIMSSDHILSLREVPKTLLVVGG
                     GVIGLEFAGIMAGLGCQVTVVEMLERLIPVDDEECSRAAQKFAQINNIEVMTGAAVTQ
                     LFPDGDAVQTEIDTGKEKIIRRFQKCLMAVGRQPNLDEQSLKKIGVDYDRKGIKTDSF
                     MQTNVKDVYAIGDCCGGYMLAHVAYAQGKIAVAHALGHNTPPMRYDNIPRVTFVHPEI
                     AVCGLTSKQLEEKGMTIKWSKFSYMASGRARAQGIKDGFFKIGVDQEGIIRSAIIVGE
                     NAGEMISFYSQAITMQTPIELLEQTIIAHPTLSEMAIEVVETAAGHPLHA"
BASE COUNT          509 a          413 c          432 g          548 t
ORIGIN      
        1 acagaagagt gtgatgcctc ggacagtttc aaggaaacat acctcgcctc aaaaaaagaa
       61 gatatagtta taatcaaaag cccggttggg atgccgggaa gggccattag taactcgttt
      121 atagaaaaag ttggcaaagg acagtgcatg ccatataaat gcccttacca ttgtatagtg
      181 acatgcgatt atcagcgcag cccttattgt ataggtcttg ccctgatgaa tgccaaaaaa
      241 gggcaaatgg agcatggatt tgcttttgct ggttcaaatg cttacagggc agacaaggtc
      301 acttcagtca aagaggtgat ggattcgatg ctccaggagt atgaggattc agcaaagcaa
      361 taagcaatag cgcggtttat attttgtaaa tggtaacacg gctcaactaa tttttgggcc
      421 gtgttttgtt tttgcaagaa ctagcattga gccaaatgct gacatgagca tttgtaaaaa
      481 tggcgagatg atgaccatca aaatcaacca gccaacgagg ctggctttgg gggtatagag
      541 gatatttggg atacctgtat ttttgaaatc aaaacctttt tcaaaaaact tcaggaaata
      601 tggccaccag atgaaaatca tctgtaccat tgtgaaaagt gaagttagaa ggcctttctt
      661 caaagaatct cttgccgatt cggctgtgtc tcctgttttt tcaatggccc ttgatgccag
      721 gaaacatccg attaggaaac ccaacagaat gtttaaaaaa ggcactccca tgcccagtcc
      781 ggtgcagatg acatcaagaa agatatataa aataactgct tttgtggggc tgatttttag
      841 tatcttttga tataatcgac gcaaaatata ttcctccaaa caaaaaaagg gaggtaaaac
      901 cccccttttt ttgttaatga tctatgcgtg caacggatgt cctgctgctg tctccactac
      961 ttcaattgcc atttcggaca gtgtcgggtg ggctatgatt gtctgttcga ggagctctat
     1021 gggggtttgc attgttattg cctgggaata gaaggagatc atctccccag cgttttctcc
     1081 tacaattatt gccgacctta tgatgccttc ctggtcaacg cctatcttga agaatccatc
     1141 ttttatgcct tgtgctcttg ccctgccaga agccatatag gagaacttgg accactttat
     1201 ggtcatgcct ttttcttcaa gttgttttga ggtgagcccg catacagcaa tttctggatg
     1261 cacaaaagtg acccttggaa tattgtcgta gcgcattggt ggtgtattat ggccaagtgc
     1321 gtgtgcaact gcaatcttgc cctgagcata agcaacatgt gccagcatgt aaccaccaca
     1381 gcaatcaccg attgcataga cgtccttgac gtttgtctgc atgaaactgt ctgtttttat
     1441 gccttttctg tcgtagtcaa cgccaatctt tttgagcgac tgctcatcga gatttggctg
     1501 tctgccaact gccatcaggc acttctggaa tctcctgatg atcttttcct tgccagtatc
     1561 aatttcagtc tggacagcat cgccatccgg aaaaagttgt gttacagcag cgccggtcat
     1621 gacttctatg ttgttgatct gggcaaattt ctgggccgcc ctggagcatt cctcatcgtc
     1681 aactggaata agcctttcaa gcatttcaac aacggtgacc tggcatccaa gtcctgccat
     1741 tattccggca aattcgaggc cgatcactcc tccaccaacc acaagcaggg tttttggaac
     1801 ttccctgagc gagaggatat gatccgagga cattatgtat ttgccatcaa ttttggcgca
     1861 tggtatgcag gccggtttgg agcctgtggc aattatcagt tt
//
LOCUS       DAOSRI010000310         1900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55834_length_1900_cov_3.937669, whole genome shotgun sequence.
ACCESSION   DAOSRI010000310 DAOSRI010000000
VERSION     DAOSRI010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55834_length_1900_cov_3.937669"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="lepB"
                     /locus_tag="PK717_07820"
     CDS             <1..436
                     /gene="lepB"
                     /locus_tag="PK717_07820"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR47602.1"
                     /translation="VTPNVDVYPPDYPDSYKMLFPFVEKGWTKDNYGPIWIPCKGATV
                     ELDADNIDFYRRIIDVYEDNDFEEQEGKYFINGEEITTYTFKQDYYFMMGDNRHNSLD
                     SRYWGFVPEDHIVGRPAVIWFSKDINKSFPKNIRWKRIFKFI"
     gene            522..764
                     /gene="rpmB"
                     /locus_tag="PK717_07825"
     CDS             522..764
                     /gene="rpmB"
                     /locus_tag="PK717_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR47603.1"
                     /translation="MARVCQVTGKKRMIGNNVSHSKRRTKREFAPNLKTKKFWSEEEQ
                     RFITLKVSCKGMKTIYKNGLSATIKKSQEKGLIKVV"
     gene            776..964
                     /gene="rpmG"
                     /locus_tag="PK717_07830"
     CDS             776..964
                     /gene="rpmG"
                     /locus_tag="PK717_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOR47604.1"
                     /translation="MSKKAKGNRVQVIMECTEQRESGVPGISRYITTKNKKNTTQRLE
                     RKKYNPYLKKVTLHREIK"
     gene            984..1145
                     /locus_tag="PK717_07835"
     CDS             984..1145
                     /locus_tag="PK717_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOR47605.1"
                     /translation="MAKKAVATFGSSRPQTKNYVKCIRMVKSERTGAYAFQEELLPTD
                     QAKDFFEKK"
     gene            complement(1216..1710)
                     /locus_tag="PK717_07840"
     CDS             complement(1216..1710)
                     /locus_tag="PK717_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR47606.1"
                     /translation="MALNKVLLIGNVGRDPEIRHLEGGASVASFSLATTERYKDRSGE
                     TKEITEWHNIVAWRQLADLASRFIKKGSQIYVEGRIRSRSWDDANGQKRYITEILADS
                     IQLLGRRDDTLPRDGAWDESVNAPKQTDSSSVAKPVSKPAVQPDQFVSSDDLLSDGPD
                     DLPF"
BASE COUNT          618 a          405 c          376 g          501 t
ORIGIN      
        1 cgtcacaccc aacgttgatg tttatccacc cgactatccc gactcctaca aaatgctctt
       61 cccctttgta gagaaaggct ggactaagga caactatgga cctatttgga ttccctgcaa
      121 gggagccacg gttgagttgg atgcagacaa tatagacttt tacaggagaa tcatagatgt
      181 gtatgaggac aacgatttcg aagagcaaga gggcaagtat ttcatcaatg gtgaagaaat
      241 aaccacatac acattcaagc aggattacta cttcatgatg ggcgacaacc gtcacaattc
      301 actggattca cgctactggg gatttgttcc tgaggatcat attgtgggcc gcccggctgt
      361 aatctggttc tccaaggata taaacaagtc tttcccaaag aacatcagat ggaaaagaat
      421 ctttaagttt atctaataaa ggctgttaaa tttggatttt agaagaaaaa tcaatacatt
      481 tgcagcccca aaaaagatta taatattaaa aatagataac aatggcacga gtttgtcaag
      541 taacaggaaa gaaaaggatg attggaaaca atgtttccca ctcaaagaga cgcacaaaac
      601 gtgaattcgc tcccaacctc aaaaccaaga aattctggtc tgaggaagag cagagattca
      661 ttacactcaa agtgtcttgc aagggtatga agaccatcta caagaacggt ctctccgcta
      721 ccatcaagaa atcgcaggaa aaaggtttga ttaaagtagt ttaatattta caactatgtc
      781 aaaaaaagct aaaggaaaca gagtacaggt cataatggag tgtactgagc aaagagagag
      841 cggtgtaccg ggcatctcca ggtatatcac aacaaaaaat aaaaagaaca ctacccagag
      901 attggagcgt aagaaatata acccttatct caagaaggta actcttcatc gcgaaatcaa
      961 ataacacact tatctttata taaatggcaa agaaagcagt ggctacattt ggtagtagcc
     1021 gtccacaaac taaaaactat gtaaagtgta tccgtatggt caaatctgag cgtaccggag
     1081 catacgcttt ccaggaagag ctgttaccta ctgaccaagc taaagacttc tttgaaaaga
     1141 aatgaagtta agtagagtaa acagacaaaa aatgcgacct tttcaggtcg cattttttgt
     1201 tgcattccaa atagtttaga agggtagatc atcaggacca tcagaaagaa gatcatccga
     1261 agaaacaaat tggtcgggct gcacagcagg tttcgatacc ggctttgcaa cactgcttga
     1321 atcagtctgt tttggagcat ttacggactc atcccatgcg ccatctctgg gcaatgtatc
     1381 atctctccta ccaagaagct ggatggaatc ggccaaaatc tccgtaatat agcgtttctg
     1441 tccgttggca tcatcccatg aacgacttct gatacgtcct tccacataga tctggctgcc
     1501 ttttttgata aacctgctgg ccaaatcagc caactgtctc caggcgacta tgttgtgcca
     1561 ctcagtaatt tctttagtct ccccactacg gtctttatat ctttccgtgg tagccaggga
     1621 aaatgaggca acagatgcac caccttcaag atgcctgatt tcaggatctc ttcctacgtt
     1681 gccaataagt aaaactttat tcagtgccat atctcaaaat tttttctaaa tataatcctt
     1741 tttgccgaga ctggaaaaaa tatttgaata caaaagagct ttttgactac ggagctctgt
     1801 caccttatcc tccagatagt ccatcagatt aagcccttct tccatttcaa ggcgtgttct
     1861 tagacgctcc cgcttattgt agatgctcat ctcattggta
//
LOCUS       DAOSRI010000311         1898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_55912_length_1898_cov_4.146500, whole genome shotgun sequence.
ACCESSION   DAOSRI010000311 DAOSRI010000000
VERSION     DAOSRI010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55912_length_1898_cov_4.146500"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1765)
                     /locus_tag="PK717_07845"
     CDS             complement(110..1765)
                     /locus_tag="PK717_07845"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOR47607.1"
                     /translation="MGRILLGADFGTSQVNVVALDVKTGQTIGFSSSPYKSGVIEKTL
                     PDGTKLPRGFALQDPSDWLEGLFEATKKLIEETRIPEKEIISIGVSFTSCTVLPVDVF
                     GTPLCMIGDLSKNPHAWPKLWRHTTAQPQANLLTTIAQERGEPFLDFYGGKMGAEWLW
                     PKLLELVQEAPELVPSIEYFIEAGDWIVWQLTGHQVRNQCAAGYKACWVEGVGYPGHD
                     YFASISQELADLSDKSRGIEVLPPGRPAGKLIKDMAGKMGLRTGIPVASAVIDAQSAV
                     AGAGVFEPNIMTIVMGSSNCHLVMSKQERLFVGYAGVVKDGILDGYWGYEAGQPAMSD
                     MLEWFVNTIAPISFYERANEDETEFATILDRYLSEVPAGSNGVLALDWMGGNRSVLLD
                     SSLSGVFAGLTLQTRAEDMYKALAEASAFGTRKIIETFRAGGVEIKQVVATGALPITS
                     PSLIQIYADVLGMPISVPDTNNATGRGAAIIGGLSVGMEKLGHKSRESYFKALLPQTL
                     DWCKPHEANKKLYNEIYNDYLTLHDMFGGTQRVLARLKERGKV"
BASE COUNT          413 a          510 c          489 g          486 t
ORIGIN      
        1 aataagtgcc agcaaaagtg cagtgattgc tttatgaatg gccgatttgt tgcttcgcat
       61 tatagacctg tttttacacc cacgcaggga aacaaaccag aaaattgggc tagaccttgc
      121 ccctctcctt gagtcttgca agtaccctct gggtcccgcc aaacatgtca tggagcgtca
      181 ggtaatcgtt gtagatttcg ttgtaaagct tcttgtttgc ctcgtgtggt ttacaccagt
      241 cgagggtttg tggaagcagg gccttgaaat agctttcccg cgatttatgg ccaagttttt
      301 ccattccgac cgacaggccg ccaatgattg cggcacccct tccggttgcg ttgtttgtgt
      361 ctggaacgga gattggcatt cccaatacat cagcatatat ctggatgagg ctgggcgagg
      421 tgattggcag tgcgcctgtt gcgacaacct gcttgatttc cacgccacca gccctgaatg
      481 tttcaattat ctttcttgtc ccgaatgcag atgcttctgc cagcgccttg tacatgtctt
      541 cggcccttgt ctgcaacgtc aggccagcga aaacaccgct cagtgacgag tcgagaagaa
      601 cactcctgtt gccgcccatc cagtcgagcg ccaggacacc gtttgagcct gccggcacct
      661 cggaaaggta tctgtccagg atggttgcaa actcggtttc gtcctcgttt gccctctcgt
      721 agaacgatat tggggcaatg gtgttcacaa accattcgag catgtcgctc atggccggct
      781 ggcctgcctc gtagccccag tatccgtcga ggatgccgtc ttttacgacg cctgcatatc
      841 caacaaaaag cctctcctgt tttgacatga caaggtggca gttcgaactg cccatcacga
      901 ttgtcattat gttgggttcg aaaacaccgg cgccggcgac ggcagactgg gcgtctatga
      961 ctgcggatgc gacaggtatt ccagtcctca ggcccatctt cccggccatg tccttgatca
     1021 gcttcccggc cggtcttcct ggtggcagca cctctatgcc cctcgacttg tcggagaggt
     1081 cggccagctc ctgcgaaatc gaggcaaaat agtcgtgacc cggataaccg acaccttcga
     1141 cccagcaggc cttgtatccg gccgcgcact ggtttctgac ctgatggcct gtcagttgcc
     1201 agacaatcca gtcccctgcc tcgatgaaat attcaattga cggaacaagc tccggtgcct
     1261 cctggacaag ctccagcaat tttggccaga gccactctgc gcccatcttt ccgccataaa
     1321 aatccaggaa gggttcgccc ctctcctgtg cgattgtggt caaaagattg gcctgtggct
     1381 gtgctgtcgt gtgcctccag agctttggcc aggcatgggg attttttgag aggtcgccta
     1441 tcatgcaaag cggtgtccca aagacatcaa cgggaaggac agtgcagctt gtgaaggaga
     1501 caccgatgga aattatctct ttctcgggta tcctggtttc ctcgatcagt ttttttgtgg
     1561 cctcaaagag cccttccagc cagtcggagg ggtcctggag ggcaaaaccc ctgggcagtt
     1621 ttgtgccatc gggaagtgtt ttttcgatca cgccggattt gtatggtgat gaggaaaagc
     1681 ctattgtctg gccagttttg acatcaagcg ccacgacatt tacctgggaa gttccgaagt
     1741 cagctcctag aagaattcta cccatgccca gattttagac catttaacct gccaaggcaa
     1801 acggccttgg caagaccgcg agtttaatca caaataaaat gcaaaaaata atttttgatg
     1861 ggtcaggaac ttgacctttt tgggggaggt ggtatcaa
//
LOCUS       DAOSRI010000312         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_56225_length_1891_cov_4.068083, whole genome shotgun sequence.
ACCESSION   DAOSRI010000312 DAOSRI010000000
VERSION     DAOSRI010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56225_length_1891_cov_4.068083"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PK717_07850"
     CDS             complement(<1..1634)
                     /locus_tag="PK717_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOR47608.1"
                     /translation="MKASITKSLSVFTILALCFGIFSFIPVSIVNAEAVEYLPFSVVF
                     TAGMHGNVKPFDYKGQPNFGGFAKAKTKIDEVRKQVLEDKTWWQYGGNTMVLDIGNTI
                     MGSQTGWFFANTKLSENQKHPTIEAMNAIKYDAMSYGAGEFSLQPAVREQLHTQSSFK
                     WINANVVVGKDRSKLTDEYQMMIYKIPTAAHPLRFGIVGLANPAVFNWEDPNNLTLGS
                     SKLALDDYVEKAREHAESLKETGEKADFIVVMTDLGLQKNADGTWKENELYRMIQEVT
                     HVDFICSSAPGQSIQQEIFTVPEPSGKIHETLVCQLPEDGVTMGRMDFVFEKCTCPVK
                     PFVFRKVNGRRDMKSRFMILDQSVNPDKDIMDKFRPQDSLLKTKFDVRIGESKCEISS
                     TNARRTDNPIAELACNVMMKETDAQFAITDIWSTTATIKKGSFTFGELFNLFPKDSKI
                     YKMTLTGQQLENALSHAATLYHTEADTNFIIGKGFNYYLDIRPGVTDRVGYMMIGNSP
                     IDPKKTYTVATNSYIALGQGGYKFEAKFEPTRKKLL"
BASE COUNT          498 a          400 c          421 g          572 t
ORIGIN      
        1 tcaaggagct tctttctggt tggttcaaat tttgcttcaa acttgtatcc accctggcca
       61 agtgcgatgt agctgttggt ggcaacagtg taggtcttct ttgggtctat tggtgaattg
      121 ccgatcatca tgtatccaac acgatcggtg accccaggtc taatgtcgag gtaatagttg
      181 aaaccttttc caatgatgaa gttggtgtcg gcttctgtgt gataaagtgt tgctgcatgg
      241 gaaagcgcat tctcaagctg ctgcccagtg agtgtcattt tgtagatttt gctgtctttg
      301 gggaaaagat taaacagttc tccaaaagtg aacgaaccct tttttattgt tgcagttgtc
      361 gaccagatat cagtgattgc aaattgcgca tcagtttctt tcatcatgac attgcaggca
      421 agttcagcaa tcgggttgtc tgttcttctt gcatttgttg acgagatctc acattttgac
      481 tcgccgatcc tgacatcaaa ctttgtctta aggagcgaat cctgcggcct aaacttgtcc
      541 atgatgtcct tgtccgggtt aacagactgg tcaagaatca tgaaacggga cttcatgtct
      601 cttctgccat tgaccttgcg gaaaacaaat ggtttgactg ggcaggtgca tttttcaaaa
      661 acgaaatcca tacgacccat ggttacgcca tcttctggga gctggcaaac cagggtttcg
      721 tgaatctttc cagatggttc cggaaccgta aaaatttcct gctgtattga ctggccagga
      781 gctgaagaac aaatgaaatc aacgtgggtt acttcctgga tcattctgta aagttcattt
      841 tctttccagg tgccatcagc attcttctgg aggccaagat ctgtcattac aacaataaag
      901 tcagcctttt caccagtttc tttgagtgac tctgcatgct ctcttgcctt ctctacataa
      961 tcgtcaaggg caagcttgga tgaaccgagg gtaaggttat ttgggtcttc ccagttgaag
     1021 acagctggat ttgccagacc aacaattccg aaacgaagtg gatgggcagc tgttgggatc
     1081 ttgtagatca tcatctgata ttcgtctgtc agttttgaac gatctttccc aacaaccaca
     1141 tttgcattta tccacttgaa ggaggattgc gtgtgtagct gctcacgcac ggccggctga
     1201 agtgagaact cgcctgcacc atagctcata gcatcatact taatggcatt catcgcttca
     1261 atggttgggt gtttctggtt ctctgacaat tttgtgtttg caaaaaacca acctgtttgg
     1321 gaacccatta tcgtgtttcc aatatcaagg accattgtat ttccaccata ctgccaccag
     1381 gtcttgtcct caagaacctg tttgcgtact tcatcaatct tggtctttgc ttttgcaaaa
     1441 ccaccgaaat ttggctggcc cttgtaatca aaaggcttca cattgccatg cattccggca
     1501 gtaaagacaa cggaaaacgg aaggtattcc acagcttcag cattcacaat cgagactgga
     1561 ataaacgaga aaatcccaaa acaaagtgcg aggattgtga aaacagacaa gcttttcgtg
     1621 atgcttgctt tcattaattt atcctccttt aaaggtaggg ttccgcattt gtgcggatta
     1681 gcgaaaccga gtataattgc ggggtagtta aaaacaagat tcacaaggaa aatttgggtt
     1741 aggttaaatg tattaacgaa acatagttta accatagtct ggcaatgctt tgtgcttggt
     1801 tgaccttcac tattgacaca atagaatcaa ttcacaatta gcttttcctg ggttaactca
     1861 ggagagggga gaacaaattg ataatgaaaa g
//
LOCUS       DAOSRI010000313         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_56478_length_1885_cov_4.578142, whole genome shotgun sequence.
ACCESSION   DAOSRI010000313 DAOSRI010000000
VERSION     DAOSRI010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56478_length_1885_cov_4.578142"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1738
                     /locus_tag="PK717_07855"
     CDS             233..1738
                     /locus_tag="PK717_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOR47609.1"
                     /translation="MTRLARLIITEIEDSRQPTLPFFDPLKVLKASRHKKEVSDTVQV
                     RNLREEQRIVDGIGDVFGKLYRDLGLDKLIHGTRRDVQWNDILESCVLARLANPSSKR
                     RSASLLEEDYGIKIPLEKIYRVMDHVADCEESIKQRISQTTLSLFGQHVDVLFFDVTT
                     LYFESIVADDLKDFGYSKDCKFKEVQVVLALVTTTGGLPISYRLFPGNTYEGKTLVAM
                     VQELQKEYSINNILLVADRAMFNEENLLEMESLGIEYIVAARLKTLPGNLKADILQSE
                     YKAAVVMDELYWVKEFGHKSRRLIVGYSSRRAKKDAADRQRLVDRLMKKVKGGKIKLK
                     DLIPNYGSKKYVRVENGSASIHQEKIESDAQWDGLHGIITNVKDKAPDELLSRYRQLW
                     QIEEAFRINKHDLKMRPIFHWTEKRIKAHIAICFLAFTLAKQAVYRVALQQDKMSFEQ
                     IRNELLHAQSSVMIDLASRKKYVIPSHVTVNQKKLYHVFGLKRSQVPYELT"
BASE COUNT          512 a          380 c          471 g          522 t
ORIGIN      
        1 tttctgcact acattgagtt tttcaataag cccccgtgta ttaattacgt tttttcaaat
       61 aataggcctg ttttaatcaa atttcaacgc ttactgcgga agacgggtta aaccaaaacc
      121 gaacgggaag agatccattc aaattgtcga aacctatcgg cgcgccgata aggtcagtca
      181 aaagatagtt cgccatgtag gtcaagcgga gacggaccgt gaagtggagg agatgacccg
      241 actggctcgc ctcattatta cagagataga agattccagg cagccaacgt tgcctttttt
      301 tgatcctctc aaggttttga aagccagccg tcacaagaaa gaggtcagcg atacggtaca
      361 ggttcgtaat cttcgtgaag agcaaaggat tgttgatggt attggagatg tgtttggcaa
      421 gctttacagg gatttgggtc tggataagct gatccatggt actcgtaggg atgtccaatg
      481 gaatgacatt ctggagtctt gtgttttggc tcgtctggcc aatccgtcaa gcaagcgccg
      541 cagcgcgtcc ttgttggaag aagactacgg tatcaagata cccttggaga agatttatcg
      601 tgttatggat catgttgccg attgtgagga gagcatcaag cagcgtattt ctcagacgac
      661 cttgtcactt tttgggcaac atgtggacgt gttgtttttt gacgtgacca ctctttactt
      721 tgagagcatt gttgccgacg atttgaagga ttttggatac agtaaggact gtaagtttaa
      781 agaggttcag gttgttttgg ctcttgtgac caccaccggt ggtttaccca ttagttacag
      841 actttttccc ggcaacacct atgagggtaa gacattggtg gcgatggttc aggagcttca
      901 aaaggaatac agcatcaaca atattttatt ggtggctgat cgtgccatgt tcaacgagga
      961 gaatttgttg gagatggaga gtctgggcat tgaatatatt gttgccgcca gactcaaaac
     1021 actgcccggt aatctcaagg cggacatatt gcagtcggaa tacaaggcag cggttgttat
     1081 ggacgagtta tattgggtca aggagttcgg gcataagtcc aggagattaa ttgtcggcta
     1141 cagttcccgg cgagccaaaa aagatgcggc tgatcgtcaa cgtttggttg atcgtttgat
     1201 gaagaaagtg aaaggcggca aaatcaagct caaagacttg attcccaatt acgggtcaaa
     1261 aaaatatgtc cgtgtggaaa acggcagtgc ctccatccat caggaaaaga tcgagtcgga
     1321 tgctcaatgg gatggtcttc acggcatcat caccaatgta aaggataaag cacctgacga
     1381 gctgctttca aggtatcgtc agctttggca aattgaagaa gcgttccgga tcaacaagca
     1441 tgatttgaaa atgcgtccca ttttccattg gaccgaaaag cgtatcaagg cacatattgc
     1501 tatttgtttt ttggccttca ctcttgccaa gcaggcagtt taccgggttg cgcttcagca
     1561 ggataaaatg agctttgagc agatccgcaa tgagttgttg catgcccaat ccagtgtgat
     1621 gattgacttg gcgtcccgaa agaaatatgt gattccttca catgtcacgg tcaaccagaa
     1681 aaaactctat catgtctttg gcctgaaaag atcacaggtg ccctatgagt taacctgatt
     1741 tataattccc tcctgttcat ttgtagtgcc ccacgttttt ttatatccct ataatcattg
     1801 tgctttttat cagatagtgt caaagtcggg actactctcc aggaaatgat ccggatcatg
     1861 gagaccagat acggtaaagc caacc
//
LOCUS       DAOSRI010000314         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_57031_length_1872_cov_3.995597, whole genome shotgun sequence.
ACCESSION   DAOSRI010000314 DAOSRI010000000
VERSION     DAOSRI010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57031_length_1872_cov_3.995597"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="tilS"
                     /locus_tag="PK717_07860"
     CDS             <1..709
                     /gene="tilS"
                     /locus_tag="PK717_07860"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOR47610.1"
                     /translation="LLCKEICLPKELKGNLEEEARILRHHALDEAAEHFRCNKIALAH
                     NATDRAETLLHNIARGAGLFGAGTMHPKTGKIVRPLLFASKRNILSYLLLLGESYCTD
                     ETNEDVRYSRNRIRKNVIPELEKIFVQAGANISRFADIAQEESNYLDVLAKDALKQCV
                     FEKEGCTFLDCSKLFEQNFVIQRRVVRILTNGSPPLSAVDSALIFFGKGENGKSEIVG
                     RARFVKVGKNIVKVLPL"
     gene            745..1266
                     /gene="hpt"
                     /locus_tag="PK717_07865"
     CDS             745..1266
                     /gene="hpt"
                     /locus_tag="PK717_07865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOR47611.1"
                     /translation="MKLEGVLIDSLAIQKRVAELACQIAMDYGEDPPVLLPVLTGSFM
                     FASDLTRNFSFDAPLHFVKVSSYGSSTVSNGKVSVSCLDKIDVSGRRVLIIEDIVDTG
                     HTIKTLVEQIKQKSPKDVKVVSLLDKPERRQVEVSVDYVGFVVPNKFIVGYGLDCDEH
                     FRCLKDIYTISEE"
     gene            1266..>1872
                     /locus_tag="PK717_07870"
     CDS             1266..>1872
                     /locus_tag="PK717_07870"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOR47612.1"
                     /translation="MNKNQDSVKTYIPIIILVAVGVLLYFFFSAFNASPKQTLTYSEF
                     YKSLQAGEISSLAIDVNSFYTEGELSPKEPGKPSIKFEVTIPPQFAEEVTHLAMEKGI
                     KVLEFEPPSSNFLLELLNYLGPFLIIIFFWFMMSRSMQGSGSQVLSFGKSKAKLFMDD
                     KPRITFKDVAGIPEVKEELQEIIDFLKDPKRFAVMGAKVPRG"
BASE COUNT          540 a          384 c          444 g          504 t
ORIGIN      
        1 tcttttatgc aaagaaattt gcctaccgaa agagttgaaa ggtaaccttg aagaggaagc
       61 aagaatattg cgccaccacg cacttgacga agccgcagaa cattttaggt gcaacaagat
      121 tgcccttgcc cataacgcaa cagacagggc agaaaccctt ttacacaata tcgccagagg
      181 cgcaggtctc tttggtgctg gcaccatgca cccaaaaact gggaagattg taaggccatt
      241 gctttttgct tccaaaagga acattttgtc ttatcttttg ttgctgggcg aatcttattg
      301 caccgatgaa acaaatgagg atgtaagata ttccaggaac aggatacgca aaaacgtcat
      361 cccggagctt gaaaagattt ttgtccaggc tggcgcaaac atctccaggt ttgcagatat
      421 tgcacaagaa gaatccaatt atcttgacgt gcttgccaaa gatgccttaa aacagtgtgt
      481 ttttgagaaa gaagggtgca ctttcttgga ttgcagtaaa ttatttgaac aaaactttgt
      541 aattcagcgt agggttgtaa gaatccttac caatggttcc ccgccgcttt ctgcagttga
      601 ttcggcatta attttttttg gaaaaggaga aaatggcaaa tcagaaattg ttggcagggc
      661 tagatttgta aaggttggaa aaaatattgt caaggttttg cccttgtgat tgataatgca
      721 caaaagtata ctctggccat gaacatgaaa ctggaaggcg tgctgattga cagccttgcg
      781 atccagaaaa gggtcgcaga gttggcctgc cagattgcca tggactatgg tgaggatccg
      841 ccggttctgc tacctgtgct gacaggttct ttcatgtttg catccgacct gacaagaaac
      901 ttctcctttg atgcaccatt gcattttgtt aaagtcagct cttatggttc ttccacagtt
      961 tccaacggaa aagtctccgt ttcatgtctg gataaaattg atgtgtccgg caggagggtc
     1021 ctgataatag aggacattgt tgatacagga cacacgatca aaactcttgt tgaacaaata
     1081 aaacaaaaaa gcccgaaaga tgtcaaagtc gtgtcgcttt tggacaaacc cgaaaggcgt
     1141 caggtcgagg tctcggttga ctatgtgggg tttgtggtgc caaacaaatt tattgttggc
     1201 tatgggctcg attgcgatga gcatttcagg tgcctaaaag acatatacac aattagtgag
     1261 gagtaatgaa caagaaccaa gacagtgtaa agacgtatat accgataata atacttgttg
     1321 cagtcggtgt tttgctgtat ttctttttct cagcttttaa tgcatccccg aaacaaacgc
     1381 tgacttacag tgaattttat aagagccttc aggcaggtga gataagctcg cttgccattg
     1441 atgtaaacag cttctacaca gaaggggagc tttccccaaa ggagcctggc aagccaagca
     1501 taaagtttga ggtcaccata ccaccgcagt ttgctgagga agtgacccat ctggccatgg
     1561 agaaaggcat caaggtgctg gagtttgagc caccttcaag caatttcctc ctcgaacttc
     1621 tgaactacct tgggccattc ctgataatca tattcttctg gtttatgatg tcacgttcca
     1681 tgcagggttc tggctcgcag gtgctcagct ttggcaaatc aaaggccaaa ctttttatgg
     1741 acgataagcc gaggatcaca ttcaaggacg tggccggcat tcccgaggta aaggaagaac
     1801 tccaggaaat aattgatttc cttaaagacc caaagcgctt tgctgtaatg ggtgccaagg
     1861 tgccaagagg tg
//
LOCUS       DAOSRI010000315         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_57764_length_1857_cov_3.175361, whole genome shotgun sequence.
ACCESSION   DAOSRI010000315 DAOSRI010000000
VERSION     DAOSRI010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57764_length_1857_cov_3.175361"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1402)
                     /locus_tag="PK717_07875"
     CDS             complement(203..1402)
                     /locus_tag="PK717_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47613.1"
                     /translation="MRKYTLVVFAVLVSFLAASCGAGPSHVPQNPFQVALSRMPKKIM
                     EVQTRKLWMKGFVFNDIRATAKSRGLATHQLLATEDLKDWLLSMGHNASSSTDSAVDL
                     GTWRHTEFLNPSTWLYNCYDISRDHELIKTEASIPDFKVWMERHNYATVATQPVLYLA
                     QSGFHRDSVVFGSGVVVGEDGAAFICREVPGDGTLEAAFGMYDDNEPSLLDVDEIREM
                     TGRMPEGLFTGLVCKLDSSVSDYNHITGGDDTGMSGLPLTSSQTAYFFEKGRPKTLSW
                     FSVAHVARKGAQGLQWEFMYDNEEEAGKDLPILEKAILETKGRFTGKNWWSEDLHLGK
                     PEITRDGKFIKVWAEFNIDGKTKEKLASGNMTPTEKKDLWFEVRALFDLSDKFLRRDY
                     GILWQGY"
     gene            complement(1399..>1857)
                     /locus_tag="PK717_07880"
     CDS             complement(1399..>1857)
                     /locus_tag="PK717_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47614.1"
                     /translation="DFPAIALIFPFESFAGKQDTRLIISGLRPDELSEMAGSYKSWGR
                     PDKIKMMGVGSKVEGLTETVKMVFLYKDEEAAEGDLEDLKNACLNARSVIDGTPWAAK
                     MGLGRAVVIRDGKMIEATFPLDRGGRSGYRFPQALDVAFRIGDYGVFWKK"
BASE COUNT          436 a          507 c          434 g          480 t
ORIGIN      
        1 gatactggcg acaaaaacga tgtgaagttg gaaaagggtg aagcgtacag gattattcta
       61 atggcatctg aagatttgga gacaaatctt tactctaacc ctttcaaatc cacagaactt
      121 gtagtcaagc tttgatggac aaatgaaaca aaaaaggcga acagcagacg ctgttcgcct
      181 tttttgttat tggcgttact ccttaatacc cctgccagag gatgccatag tccctgcgca
      241 aaaacttgtc agaaaggtca aagagcgccc tgacctcaaa ccacaggtct ttcttttctg
      301 tcggtgtcat gttgcctgat gcaagttttt cttttgtttt gccatcaata ttgaactcgg
      361 cccagacttt tatgaatttt ccgtcccttg tgatctctgg cttgccaagg tgaaggtcct
      421 cgctccacca gttcttgcct gtgaagcgcc cctttgtttc cagaatagcc ttttcaagaa
      481 tgggcaaatc tttcccagcc tcttcctcat tgtcgtacat gaactcccac tggaggcctt
      541 gggcgccttt ccttgccaca tgggcaacag aaaaccagga aagcgtcttt ggtcttccct
      601 tttcaaaaaa ataggctgtc tgggaactgg tgagtggcaa acctgacatg ccggtgtcgt
      661 caccgcctgt tatgtgattg tagtcagaaa cggatgaatc cagcttgcac acaaggccag
      721 taaacagacc ctccggcatc ctgccggtca tctcccttat ttcatccaca tctagcaggg
      781 aaggttcatt gtcgtcatac atgccaaagg cagcctcaag cgtcccgtca cctggtactt
      841 ctcggcagat gaacgccgca ccgtcttcac caaccacaac accggagcca aaaacgaccg
      901 agtccctgtg gaacccgctc tgtgcaagat aaaggacagg ctgggtggcc accgtcgcat
      961 agttgtgcct ctccatccat accttgaaat ccgggatgct ggcttctgtc tttatcagct
     1021 cgtggtccct ggaaatgtca tagcagttgt aaagccaggt gctgggattg aggaattcgg
     1081 tgtgcctcca cgtgccaagg tcaactgccg aatccgtcga ggaggaggca ttgtggccca
     1141 tggagaggag ccagtccttg aggtcttctg ttgcaaggag ctggtgtgtg gcaagccccc
     1201 ttgatttagc ggttgccctt atatcattga agacaaagcc cttcatccag agttttcgtg
     1261 tctgcacttc cattattttt tttggcatcc tggagagtgc aacctgaaaa gggttttgcg
     1321 gcacatggga cggaccagcc ccgcatgagg cagccaaaaa ggacaccaaa acggcaaaaa
     1381 caacaagggt atattttctc attttttcca gaacactccg taatcaccta tcctgaaggc
     1441 aacatcaagc gcctgcggga accggtatcc actccggcct cccctgtcaa gcgggaatgt
     1501 ggcctcaatc atttttccgt cgcgtatgac tactgccctg ccaaggccca tttttgccgc
     1561 ccacggcgtg ccatcgatca cactcctggc attcaggcat gcgtttttga ggtcttcgag
     1621 gtcgccttcg gcggcctctt cgtccttgta caggaacacc attttgacgg tttctgtcaa
     1681 accctcaacc tttgaaccga cacccatcat ttttatcttg tccggccttc cccaggactt
     1741 gtagctgcct gccatttcgg acagctcgtc tggcctcagc cctgaaatga tgagccttgt
     1801 gtcctgcttc ccagcgaagg attcaaaggg aaaaatcagg gcaattgccg gaaaatc
//
LOCUS       DAOSRI010000316         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_57798_length_1856_cov_3.635758, whole genome shotgun sequence.
ACCESSION   DAOSRI010000316 DAOSRI010000000
VERSION     DAOSRI010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57798_length_1856_cov_3.635758"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>310)
                     /locus_tag="PK717_07885"
     CDS             complement(129..>310)
                     /locus_tag="PK717_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOR47615.1"
                     /translation="DRMLGIRAGIAAMEWLLSGDCGIMAALRGNEIIKVPLKDAVGSL
                     KTVTGDWIRLLEVLE"
     assembly_gap    311..320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(321..1149)
                     /locus_tag="PK717_07890"
                     /pseudo
     CDS             complement(321..1149)
                     /locus_tag="PK717_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
     gene            complement(1160..>1856)
                     /locus_tag="PK717_07895"
     CDS             complement(1160..>1856)
                     /locus_tag="PK717_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOR47616.1"
                     /translation="SLFAPQKQEEEPPTPKEEILAMEKEIMGFYMSDHPMRPYEKYIA
                     TIDHTDIAALGEVEEGSSIVILGIITSFRRAKSKKGNPISTCTLEDLTGICSITAFGK
                     AYETLSDHLEDEGIVMIRGKVESKEGQPKIIAEEYIKTINTSDIQVSKGDETFVINVQ
                     VNLETQYHLLEKIKKLAMSKGNGQNPLVLFLAKGGQKIRMETDKECWIADHSVIPAFK
                     ELVGPNQVWLSKN"
BASE COUNT          391 a          492 c          394 g          569 t
ORIGIN      
        1 ggtttcaagg gtcttttttg attggctgga tgctatatat ctgaaaacaa gggctccggc
       61 aataatgaac agaacaagaa aaaatacgac ctggaatctt atcgagctaa gaagctttga
      121 aatcgctttt attccagcac ctccaaaagc ctgatccagt caccagtgac tgttttgagt
      181 gacccaaccg cgtctttgag tgggactttt ataatctcgt tgcccctgag tgcggccatt
      241 ataccgcaat cccctgagag gagccattcc atggccgcta tcccggccct tattccgagc
      301 atcctgtcaa nnnnnnnnnn gtgttggcgg accacccctt tcgatgtgcc caagctttgc
      361 agagcgggtc tcgatccctg ttcttttacc aatttcttcg gctagctttt cgccaacatc
      421 ctgttttgcc agattggtat gtccaaattc gtcagtctct gattggccag atgcaagctc
      481 aacgccttcc gaaacaacca caatggcata gcccctttct gcatgggcct ttgaaacctt
      541 ggaacaaatt tcgtcccagc tccattttct ttccggaatc attgtgtagt ctgcgtaagc
      601 tgatattcct gtctaaagtg ccacccatcc cgcgtgcctt cccatgactt cgagaacaag
      661 ggcccttcca tgggattttg ccgtatcacg cagcctcatt gcggcatcca ttccaactgt
      721 tgctgcagaa tcgaatccga atgttgagtc tgttcccgag agatcattgt ccatggtttt
      781 tggcacacca accaccggaa ggccaatatc ataaagtttt gatgccactc caagcgtgtc
      841 ttcgccccca gcggccacaa ggccgtcaag cgaaagcgat tcaaaagtct tttttactgc
      901 atcgacgccg ccctctcttt tgaacgggtt tgttcttgag gtgcgaagga tagttcctcc
      961 atggcccact atgtcttcaa tagctttacg attgagttcg atgaaatttc cctcgatcat
     1021 gcccttccag ccctcgagga tgccaaatac tttatatccc tctttcaagg cccttgttgt
     1081 tgcgccgtat attgcggggt tgagccctgg gcaatcgcca ccgcctgtca gtatggccac
     1141 ttttttcatc ttcaccacct caattttttg aaagccaaac ctggtttggg ccaaccagct
     1201 ccttgaaagc tggtatgacc gaatggtctg cgatccagca ttctttgtct gtctccatcc
     1261 ttattttctg cccgcccttg gccaggaaca gcacaagggg gttttgcccg ttaccctttg
     1321 acattgccag cttctttatc ttttcaagga ggtggtactg ggtttcaagg ttcacctgga
     1381 catttattac aaacgtttca tcaccttttg aaacctgaat gtcggaagtg tttatcgttt
     1441 ttatgtattc ctcagcgata atctttggtt ggccttcctt gctctcgact ttccccctta
     1501 tcatcactat tccttcgtcc tcaaggtggt cagagagtgt ctcgtaagcc tttccaaatg
     1561 ccgttataga gcatattcca gtcaagtctt caagggtgca tgtggaaatc gggttgcctt
     1621 tttttgattt tgccctcctg aagcttgtga ttatcccaag aatgacgatt gagcttccct
     1681 cttcaacctc tcccagcgcg gcaatgtcgg tatggtcgat tgtagcaatg tatttttcat
     1741 agggtctcat cgggtgatcc gacatgtaga aacccattat ttctttttcc atcgccagta
     1801 tttcttcttt tggtgttggt ggttcttctt cctgcttctg tggggcgaaa agcgag
//
LOCUS       DAOSRI010000317         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_58476_length_1842_cov_2.551203, whole genome shotgun sequence.
ACCESSION   DAOSRI010000317 DAOSRI010000000
VERSION     DAOSRI010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58476_length_1842_cov_2.551203"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PK717_07900"
     CDS             complement(<1..796)
                     /locus_tag="PK717_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOR47617.1"
                     /translation="MSRSGHTYLDHLTDFGIKLGLDKMRFILTKLGNPQKKYSSILIA
                     GTNGKGSVANFITNCLKENNYKVGFYTSPHLIRVEERIRINGKPIPSRIFDNLCLEIK
                     GLCMKLPIEMQPTYFEALTAVAFEYFAREKIDVCVCEVGMGGRFDATNVIDADLQIIT
                     PVSYDHMEYLGNSIQEIAFEKAGIIKQDAIVISGKQSLEAEKVLKRICKQRNSRIFLY
                     GKDFSARTVHSCSLDGQVFDFTGIEKLKNVSIKMLGRYQVHNAAVAL"
     gene            complement(817..978)
                     /locus_tag="PK717_07905"
                     /pseudo
     CDS             complement(817..978)
                     /locus_tag="PK717_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204936.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1017..>1842)
                     /locus_tag="PK717_07910"
     CDS             complement(1017..>1842)
                     /locus_tag="PK717_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR47618.1"
                     /translation="IFCERAGIVNIGIEGQMLMSAFLSYMTNVWTSNLLLSLLVGMLT
                     GAIMGLFLAFLSIKLKLNQTIGGTVINIMATGITGYFYQTGLTTQGKLWNIPLGPLAK
                     IPLIGPVLFNNPPITYMAILLVIFSQVLLFHTRWGLRTRAVGEHPRAADTVGVDVFKI
                     RYWNVIIGGIMAGLAGSFLTLEGVGMFERGMTNGRGFIALAVMIFGKWNPISSALAAL
                     FFGFANAMQIQLQFMGIQIPHQFVGMIPYLLTIIVVSGFVGRSRAPAAEGIPYEKE"
BASE COUNT          578 a          420 c          329 g          515 t
ORIGIN      
        1 caagggcgac tgctgcatta tgaacctggt acctcccaag catttttatt gatacatttt
       61 ttagtttttc tatgcctgta aaatcgaaaa cctgtccatc aagagaacaa gaatgaacag
      121 tccttgctga aaaatctttt ccatataaaa aaatcctcga attacgttgt ttacaaattc
      181 ttttaagaac cttttctgct tctaaactct gtttcccaga aataactatg gcatcttgtt
      241 ttatgatgcc tgccttttca aaggcaatct cctgaatact gtttccaaga tattccatat
      301 ggtcgtatga aacaggtgtt ataatctgca ggtcagcatc aataacattt gtagcatcaa
      361 accttccacc cattccaacc tcgcaaacac aaacatctat tttttccctt gcaaaatatt
      421 caaaagcaac agcagtaagt gcttcaaaat atgttggctg catttcgatt ggtagtttca
      481 tacagagacc tttaatctcg agacaaagat tatcaaaaat tctggacgga attggctttc
      541 catttatcct tattctttcc tctaccctga taagatgagg acttgtatag aaacctacct
      601 tataattatt ttctttcaaa caatttgtaa taaaatttgc aactgaaccc tttccgtttg
      661 tacctgcaat taaaatagaa gagtattttt tctgaggatt tccgagtttt gtaagtataa
      721 aacgcatttt atcaagccca agttttatac caaagtcagt tagatgatca agatatgtat
      781 ggccagatct actcattgag aggatgcaaa aatagaaagg atttttataa gggttgattt
      841 tcccgcgcca ttttcaccca gtaatgccag tacctcgcct ttgcgcagtt caaggctcac
      901 atcgtcattt gcgaccgtac ccggaaaagt cttgtaaata tgttttgctt ctaagataac
      961 gcttttatca gtatccataa cggcatcagc tgttttagaa tatgtattgt ccatacttac
     1021 tccttctcgt agggaatgcc ttcagccgcg ggcgcacgcg acctgcccac aaagccagaa
     1081 actacaataa tggtcagcag atatggaatc atacccacaa actgatgtgg aatttgaatt
     1141 cccatgaact gtaactgaat ttgcatcgcg ttagcgaagc caaagaacaa ggctgccagc
     1201 gcggatgaaa tcgggttcca ttttccaaaa atcatcactg ccaaggcgat gaagccacgt
     1261 ccgttggtca tgcctcgttc aaacatgcca acaccttcca gggttaaaaa tgaaccagcc
     1321 aagccagcca ttatgccacc aataatcaca ttccaataac ggattttgaa gacatcaaca
     1381 ccaacggtgt cagccgctcg gggatgttcc cctaccgcgc gggtgcgcaa accccaacgg
     1441 gtatggaaca aaagcacctg cgagaaaatg acaagcaaga tcgccatata ggtgatggga
     1501 gggttattaa acaaaactgg tccgattaac gggattttag ccaaagggcc caaaggaata
     1561 ttccacaatt taccctgtgt ggtcaagcca gtttgataaa aatagcccgt aatacccgtt
     1621 gccataatat tgatgactgt gccaccaata gtttgattga gcttcagctt aatcgacaag
     1681 aaggctaaaa atagccccat aatcgctcca gttaacatgc ccaccagcaa ggacaaaagc
     1741 aaattgctgg tccaaacatt agtcatgtag gaaagaaaag cggacattaa catctgccct
     1801 tcaataccaa tattcacaat tcctgcccgt tcacagaaaa ta
//
LOCUS       DAOSRI010000318         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_58622_length_1839_cov_3.349215, whole genome shotgun sequence.
ACCESSION   DAOSRI010000318 DAOSRI010000000
VERSION     DAOSRI010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58622_length_1839_cov_3.349215"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PK717_07915"
     CDS             complement(<1..332)
                     /locus_tag="PK717_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR47619.1"
                     /translation="MQEFDAASLMKRIAKGDEDAFRCLYDEFGAYVFKIVAMQLHDNG
                     KAEELTQDVFLKIWRFASKYDPSRPFKPWLSRVISNEIISYRRAQEERTESIEELAET
                     GYTPAEEM"
     gene            complement(380..1813)
                     /gene="gatB"
                     /locus_tag="PK717_07920"
     CDS             complement(380..1813)
                     /gene="gatB"
                     /locus_tag="PK717_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOR47620.1"
                     /translation="MSDLVIGIEVHVQLLSATKMFCGCKNSFGDEPNTNVCPVCLGLP
                     GALPMVNEKASEMGLQLALALNCKINEVAPYYRKNYFYPDLPKGYQITQYTTPLGQKG
                     SLEFPFGKGKKRVDITRIHLEEDAGKMFHSGDITKSSSSLVDYNRCGAPLAEIVTEPC
                     IETPEEAQAYLFALRNLVRFIGISTGNMEEGALRCDVNVSVKNPDGSFGTKVEVKNLN
                     SFRSARRALEFEAKRQRDLIAKGGQVTAETRHFIESTGETAGMRSKEELNDYRYFPEP
                     DLPPMITGADQIEKIRQEMPKTPGQIAEKFEKEYGLSQYDSGVLTQDKETTVFFEECV
                     KNGASPKKTCSFLTVEIQGYFAKEGLSLGQTKLTPKLLSELVKLVDSNQITNQSAKTI
                     IPDVVAGASPEKVAKERNLIAIEDESAIIELVRQVIENNPKQVEQYKQGKTALSGFFI
                     GQVLKASCGKAQAETVKRLVEEALSRL"
BASE COUNT          376 a          441 c          430 g          592 t
ORIGIN      
        1 tccatctctt cagctggggt gtagccagtt tctgccagct cctcaatact ttcagttctc
       61 tcttcctgcg cccttctgta gcttatgatt tcgttactta tgacccgaga gagccatggc
      121 ttgaagggcc ttgaaggatc atatttcgat gcaaatctcc atattttgag gaagacgtcc
      181 tgggtgagtt cctccgcttt accattgtca tgaagttgca ttgcgacaat cttgaaaacg
      241 tatgcgccaa actcgtcgta gaggcatcga aaagcgtcct catcgccttt tgctatcctt
      301 ttcatgaggc tggcggcatc aaattcttgc attatggttt atttttggtt ttgggacttc
      361 tcttacgagg cattgttggt taaagccttg agagtgcttc ttcgacaagt cgtttcaccg
      421 tttcagcctg ggcttttcca caagaagctt tgagcacctg cccaatgaag aaccctgaaa
      481 gggcagtttt gccctgctta tattgttcga cttgtttcgg attattttcg attacctgcc
      541 tgacaagttc gatgattgcg gactcgtctt ctattgctat taaattgcgc tcttttgcaa
      601 ccttttctgg gctggcacca gcaacaacat cagggatgat tgtttttgct gactggtttg
      661 ttatctggtt tgagtccacc agcttcacca gctccgaaag cagttttggg gtcagttttg
      721 tctggccaag cgatagcccc tcttttgcaa agtagccctg gatttccacc gtaaggaaac
      781 tgcaggtttt tttggggctg gcgccgtttt tgacacattc ttcaaaaaac acggttgttt
      841 ctttatcttg tgttagaaca cctgaatcat attgtgagag cccgtattct ttctcaaact
      901 tttctgcgat ctggccaggt gtttttggca tttcctgtct gattttttcg atttggtccg
      961 cgcccgtgat catcggtgga aggtctggct ccgggaagta cctgtagtcg ttgagctcct
     1021 cttttgagcg catccccgca gtttcgcctg tggactcgat aaagtgcctt gtttcggcag
     1081 ttacctgccc acccttggca atgaggtccc tctggcgttt cgcctcaaat tcgagtgctc
     1141 ttcttgctga cctgaaggaa ttgaggtttt ttacttccac ctttgtgccg aaagacccgt
     1201 ccggattttt taccgacacg tttacgtcac acctgagtgc gccctcttcc atgttgccag
     1261 ttgaaatgcc aatgaaccgg acgagatttc tcaaggcaaa aaggtatgcc tgggcctctt
     1321 cgggggtctc aatgcaaggc tctgtaacaa tctctgcaag gggagcgcca catctgttgt
     1381 agtcaaccag cgacgaggac gattttgtta tgtcccctga gtggaacatt tttccagcgt
     1441 cttcttcaag gtggatcctc gttatatcga cccttttttt gccttttccg aatgggaatt
     1501 caagggatcc tttttgtccc agaggtgtgg tatactgggt tatctgatag ccctttggaa
     1561 ggtccggata gaaatagttt tttcggtaat atggtgccac ttcgtttatc ttgcagttta
     1621 gcgctagggc aagctggagg cccatttcag aggctttttc gttgaccatt ggcagggcgc
     1681 ctggcaatcc gaggcaaact gggcacacat ttgtatttgg ctcgtcgcca aacgaattct
     1741 tgcaaccaca aaacatcttt gttgcagaca aaagttgcac atggacttca attcctatta
     1801 caagatcaga cattgccgct cctgaggttt tcaatgagt
//
LOCUS       DAOSRI010000319         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_58787_length_1835_cov_3.625281, whole genome shotgun sequence.
ACCESSION   DAOSRI010000319 DAOSRI010000000
VERSION     DAOSRI010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58787_length_1835_cov_3.625281"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PK717_07925"
     CDS             complement(<1..1305)
                     /locus_tag="PK717_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOR47621.1"
                     /translation="MMRRRLISIVLVLYLLVPQFITTAVQSKDNEGLWIEGTQQNPRP
                     TNGVATIKGFTGKVTVTESSYEFDLICELVCETPVDKMQWYVATSELAIKRATLDDRE
                     IKSRSDKGNFFLEFPQKMQTGAHKLRFIYDGTMNYASPEGIRTQIFTNGYHFHCYNAW
                     YPMSDSISTDKQVTFDLDVKIPSHWFLLGSFVPKEYRDKPKSDGKYKVEIKTENIYAA
                     KLVGGDYIVFKEEGEKYAVRVYGFRGGNTSFKTILKTAKSTADFFGQYYNKPCENDYF
                     VAAQTGRRGNGQGLDGGWVIDSPYLNEESFAPDFLAHECAHLSWFGGNGIIPNPKVHP
                     DQRVLSEAFAEFSAILYCDSLSPEQGQAKLKSVRDIYFKNKKDYEPPLSSTACEWVDY
                     IVYQKGALVVWALKGKLGDDVFKSAMQEFMKKYDYLIEIYYVL"
BASE COUNT          503 a          382 c          340 g          610 t
ORIGIN      
        1 aagaacataa tatatttcaa tcaaataatc atattttttc ataaactcct gcattgcact
       61 tttgaacaca tcatcgccca gttttccctt aagtgcccaa acaacaagtg cacccttctg
      121 gtagacgata tagtcgaccc attcgcatgc agtgcttgaa agtggcggct cataatcttt
      181 tttatttttg aagtaaatat cccttacact tttcagtttt gcctgccctt gctctggaga
      241 caatgaatcg caatagagaa ttgctgagaa ttcagcaaag gcttccgaaa gcactctttg
      301 atctgggtgt acctttggat tgggtatgat gccgttgcca ccaaaccacg acaaatgggc
      361 acactcatgg gccagaaaat ccggagcaaa tgactcttcg ttgaggtatg gggagtctat
      421 gacccatccg ccatccagcc cctgaccatt gccccttctg cctgtttgtg cagctacgaa
      481 atagtcgttt tcacagggtt tgttgtagta ttgcccgaaa aagtcagcag ttgattttgc
      541 agttttcaat atggttttga aagaagtatt gccacccctg aaaccgtaga ctcttacagc
      601 atacttctcc ccctcttcct tgaaaactat gtagtctccg ccaaccagct ttgcggcata
      661 tatgttctcg gtttttatct caaccttgta cttcccatcg ctttttggtt tatcgcggta
      721 ttcttttggc acaaaactcc ccagcagaaa ccagtgggag ggtattttga cgtcaagatc
      781 gaatgtcacc tgtttatctg tggaaatgct gtctgacatt ggataccatg cattatagca
      841 gtgaaaatgg tagccatttg taaatatctg tgttcttata ccctctggac tcgcgtaatt
      901 cattgttcca tcgtagatga atcttagctt gtgcgcgccg gtttgcattt tttgagggaa
      961 ttcaaggaag aaattgcctt tatcacttcg ggattttatt tctctgtcgt caagtgttgc
     1021 cctctttatt gccagttccg atgtcgcaac gtaccattgc attttgtcaa caggtgtttc
     1081 acacaccagt tcgcagatga ggtcaaattc ataggatgat tcggtgacag tgacttttcc
     1141 ggtgaaacct tttattgttg ctacgccatt tgttggtctt gggttttgct gggttccctc
     1201 aatccataga ccttcattat ccttggattg tacagctgtt gtaataaatt gtggaacaag
     1261 aagataaagg actaatacaa tcgaaatcag tcttctgcgc atcatcaacc tcctctatag
     1321 aatagtaatg gttttctggt gctcaggttg tttacaaaat gtatacaaaa ttggttcttt
     1381 gttttttggc aaaatagata aaccctcaga aagtaaaagg tttatcttgt ccccctatcc
     1441 agccggaccc atttgatttt gattataagt gtaattttac tgcaccaaat tcaagatggt
     1501 gaaaaaacat tgcaaaaatc taaatatttt tgcaatgtgc aaagaagatt tttttaagca
     1561 tatttaaaag tcatcatgat tacacaattt gtttgtttta taagtgaaaa ttaatttatc
     1621 acagaaaagc actggattta actaataata ctttatattt aacaatatga gcacaattaa
     1681 tttatatttt ttatccacaa tttattagtc atggttatac attgcaatct tagtcctgcc
     1741 agtaacctat tgtgacctac tgcgtgtctt tgtcccagac aatgtctgcg ccaaagactt
     1801 cagatatgaa tcttattgga acaagagtaa ctcca
//
LOCUS       DAOSRI010000320         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_58788_length_1835_cov_3.565730, whole genome shotgun sequence.
ACCESSION   DAOSRI010000320 DAOSRI010000000
VERSION     DAOSRI010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58788_length_1835_cov_3.565730"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1423)
                     /locus_tag="PK717_07930"
     CDS             complement(122..1423)
                     /locus_tag="PK717_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47622.1"
                     /translation="MSDFKIKTVWTKSDDNDAIFKNTTANLSISVAETFLTQNINGWT
                     KSVQDSILVSTYPLAKWFAYYWWRHENEFLTINDKKPDFNWRIAHELGAANQGYVWPK
                     VLFASDGEFINIWSDIIPTPHQSVNYIGKLDAVQYVPIVTFQKEVESLIEATVERITG
                     LDEDLPELWKIVCEERRDPGTSNKRKLEAVLGYDPEECPEEILQRILPFQEDVGVSSI
                     KEIAPFFGSDSRLQQRLQTEQGIESNPQVQQDQIKIDRTRDILPWQQGVSAARQLRKV
                     CDLGDGVVENNTLLELLGIASNGLSDYNNYSVESPVSVGKNERNGKWTFIPREKRFET
                     SQRFELARLLGDLLTYQDSNKEWLVTSDYKSSRQKAQRAFAGEFLCPIQALDDYLKGD
                     YSETRREKAANDFHVSIQTIDTLLLNNGKIERDDKVFPYSA"
     gene            complement(1517..1657)
                     /locus_tag="PK717_07935"
     CDS             complement(1517..1657)
                     /locus_tag="PK717_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47623.1"
                     /translation="MRARGEKLLFYPLMTQMTQIFWGCVVQGKLNRAAAEMLKARTTC
                     GV"
     gene            complement(1654..>1835)
                     /locus_tag="PK717_07940"
     CDS             complement(1654..>1835)
                     /locus_tag="PK717_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR47624.1"
                     /translation="QPELLVSVQDQIVTSSQAMRAKLRDPEKNVAEEEDFPCAPSEYG
                     CEQCSFREVCPALSR"
BASE COUNT          470 a          451 c          368 g          546 t
ORIGIN      
        1 tacttagggt cgggcaatgt gcaaatccaa tggtgcccta ggcgcctact aggcgtctac
       61 cagcccaacc actgtgccaa gtcgctcttt ccatcgcaaa agttcgcaca ttgctacaga
      121 tttaggcaga atacgggaaa accttatcgt ctctttcaat ttttccatta ttcaatagta
      181 gtgtgtctat agtttgaatg ctgacatgga agtcgttagc tgctttttcc cgccttgttt
      241 cagaataatc gcctttgaga taatcatcca aagcctggat tggacaaagg aattcaccgg
      301 caaatgctct ttgagccttc tgacgagaag atttataatc agaagtcacc agccactcct
      361 tgttggaatc ttggtaagtt aacaagtcac caagaagccg cgccaattca aatctttgac
      421 ttgtttcaaa tcttttttcc ctgggaataa atgtccattt cccattcctt tcattcttac
      481 caacagaaac aggactttca acagagtagt tgttgtaatc agaaagccca ttggaagcaa
      541 ttccaagcag ctctaataat gtattgttct cgacaactcc atctcctaaa tcacaaactt
      601 tcctgagttg ccgcgccgcg cttacgcctt gttgccacgg aagtatatct cgggttctgt
      661 ctatcttaat ttggtcttgc tgaacctgtg gattgctttc aattccttgc tctgtttgca
      721 gacgctgttg cagcctgctg tccgaaccaa aaaaaggagc aatttcttta atagatgata
      781 cgcctacatc ctcttgaaaa ggcaaaattc gctggagaat ctcttcggga cattcttctg
      841 gatcatagcc gaggacagcc tcaagttttc ttttgttact ggtaccagga tcacgacgtt
      901 cttcacaaac gattttccac aattccggta agtcttcatc tagtccagtt attctttcaa
      961 cagttgcctc aatcaaggac tcaacttcct tttgaaaagt cacaatagga acatattgaa
     1021 cagcatccag cttcccgata taattcaccg actggtgagg cgtcgggatt atgtcagacc
     1081 agatattgat aaattcgcca tctgacgcaa aaagaacctt tggccagaca tatccttggt
     1141 ttgccgcgcc aagttcatga gcgatgcgcc aattgaaatc tggctttttg tcattaatgg
     1201 ttaggaattc attttcatgc cgccaccaat aataggcaaa ccacttggcc agagggtaag
     1261 tcgagacaag aatagaatct tgaacacttt tcgtccaacc attgatattt tgagtaagaa
     1321 aggtctcagc gacacttatg gaaagattcg ctgttgtatt cttgaaaatg gcatcattgt
     1381 cgtcagactt tgtccaaacc gtctttattt tgaaatcaga catttcgacg tcctccattt
     1441 ttcaagtatt ttctcataca tgggatccgt tggttgcaac ggatagccat gtcttgtgat
     1501 gaatacttgt atgccttcag acaccgcatg tcgttctggc tttcagcatt tctgctgctg
     1561 cacggtttaa tttcccctgc acaacacacc cccaaaaaat ctgcgtcatc tgcgtcatca
     1621 gcggataaaa caacagtttc tcgccccttg ccctcatcta ctgagagctg ggcagacctc
     1681 tctgaagctg cattgttcgc agccatattc cgagggtgcg cacgggaagt cttcttcttc
     1741 ggcgacattt ttttccgggt cacgcaattt agcgcgcatt gcctgcgagc tggtgacgat
     1801 ctggtcctgt accgagacca gcagctcggg ctgca
//
LOCUS       DAOSRI010000321         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_59032_length_1830_cov_2.985915, whole genome shotgun sequence.
ACCESSION   DAOSRI010000321 DAOSRI010000000
VERSION     DAOSRI010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59032_length_1830_cov_2.985915"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK717_07945"
     CDS             <1..209
                     /locus_tag="PK717_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47625.1"
                     /translation="NLQLLLSYAKPLLAPGGSLVLWKGRDDISELPSMLPKIEKLGFS
                     LIKTIPYKIPLWHLERFLVHLERL"
     gene            253..1437
                     /locus_tag="PK717_07950"
     CDS             253..1437
                     /locus_tag="PK717_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR47626.1"
                     /translation="MSGFDGRLQAFKERKEETIRDKHMYYLNEIQAHEGQFVVIGGKK
                     YLNFTTYSYLGLLGHPKINEAAKAAIDRFGSGTHGVRILAGTTSIHVELENRIATYKK
                     TDDSIAYSSGYVTNLSTISTLTGNGDEIFCDIINHASIYDGCLLSRAKFTRFKHNDME
                     DLENHLKNSKAVGKLIVVDAVFSMDGDIAPLPKLIELARKYDCLLMVDEAHSLGVLGK
                     TGHGIMEHFGLKEGIDIQMGTLSKTIPAIGGYIAAKKDIIDLLKYNARAFVFSAALPP
                     PAAEAARVSFDVLEEEQWRLERLRDNISYYIKSLNQAGFDTGVSQTAIVPIILKDEWM
                     TLETTRLLFEEGVFIMPILPPAVPPNTSRLRANVTASHTREDIDYMVDALSRVWKKLG
                     MR"
     gene            complement(1386..>1830)
                     /locus_tag="PK717_07955"
     CDS             complement(1386..>1830)
                     /locus_tag="PK717_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47627.1"
                     /translation="NATDDELAIEELSKRGFGEINIVGLTGGKAGRPDHVLFNYWLFW
                     LENKLGIKLRGFCEGFEIFSVSGCCHIKCKKGMTVSILPFFGTTNMKLSGLKYPFEGK
                     IEPGPARWVSNLALGDFTIESEGKVLVFVQRIPNFFQTLERASTI"
BASE COUNT          533 a          401 c          390 g          506 t
ORIGIN      
        1 gcaacttgca attgcttctc tcttatgcaa agcctttgct tgccccaggc ggttcgcttg
       61 tactttggaa aggcagggat gatatttcag aattgccctc gatgcttcca aaaatagaga
      121 aattgggttt tagtttgatc aaaacaatcc cgtataaaat tccattatgg cacctagaac
      181 gcttcctcgt acacctggaa cgcttgtaaa aggtgttcac tcatataaaa tgtgcttttc
      241 aaggaggtat cgatgagtgg tttcgatggc agattgcaag cctttaaaga gagaaaagaa
      301 gaaacaatac gtgacaaaca catgtattat cttaatgaaa tacaggcaca cgaaggccag
      361 tttgttgtca ttggtgggaa aaaatacctg aattttacca catactccta ccttggcctt
      421 ttggggcacc ccaagataaa tgaagcagcc aaagctgcaa tagacaggtt tggttccgga
      481 acacatggtg tgagaatcct tgcgggaacc acatcgatac atgtcgagct agaaaataga
      541 attgccactt ataaaaagac tgatgattcc atagcttact catcgggata cgtcaccaat
      601 ctttccacca tttcaacact caccggaaat ggtgacgaga ttttttgcga tataataaat
      661 cacgcttcga tatatgatgg ttgcttgctc tcaagggcaa aattcaccag gtttaaacat
      721 aatgatatgg aagatcttga gaatcacctc aagaattcga aagccgtcgg taaattgatt
      781 gttgttgatg ctgttttctc aatggatggt gatattgctc cactaccaaa actgattgaa
      841 ctggcaagaa aatacgattg tctccttatg gttgatgaag cccattcgct tggtgtgctt
      901 ggcaaaacag ggcatgggat catggaacat tttggcctca aagaaggaat tgacatccag
      961 atgggcacct tgtcgaagac tatccctgca atcggtggct acatagcggc caaaaaagat
     1021 ataattgatc tgctcaaata taacgccagg gcatttgttt tttcagcagc acttccaccg
     1081 ccagcagcag aagcggccag ggtttctttt gatgttctcg aggaagaaca gtggagactc
     1141 gaaagactca gggacaatat ttcctactat atcaaatcgc tcaaccaggc aggttttgat
     1201 acgggtgtaa gccagacagc catagtcccg atcatcctaa aagacgagtg gatgaccctt
     1261 gaaacaacaa gactcctctt cgaggaaggt gtttttataa tgccgattct tccaccagca
     1321 gttccgccaa atacttcaag gctcagggcc aatgttacag cctctcatac cagggaagac
     1381 attgattata tggttgatgc tctttccagg gtttggaaaa aattgggaat gcgctgaacg
     1441 aaaaccaaca ctttcccctc tgattcaatt gtaaaatcgc ccagtgccag attactcacc
     1501 catctagctg ggcccggctc tatttttcct tcgaatgggt atttaaggcc agacagtttc
     1561 atatttgttg tgccaaaaaa aggcaggatg ctgaccgtca tgcccttttt gcatttgatg
     1621 tggcaacatc ctgaaactga gaagatttca aagccttcgc aaaatcctct taattttatt
     1681 ccgagcttgt tttcaagcca aaaaagccag taattgaaga gaacatggtc tggccttcct
     1741 gctttaccac ctgtgaggcc gacaatgttt atttccccaa aacccctttt tgagagctct
     1801 tctatggcca attcgtcatc tgtggcgttt
//
LOCUS       DAOSRI010000322         1812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_59873_length_1812_cov_3.496301, whole genome shotgun sequence.
ACCESSION   DAOSRI010000322 DAOSRI010000000
VERSION     DAOSRI010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59873_length_1812_cov_3.496301"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK717_07960"
     CDS             <1..520
                     /locus_tag="PK717_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47628.1"
                     /translation="GMRQKAALARAMLCEPEILFLDEPTANLDPVSSAGFRDLILELA
                     RKRGTTVFLNTHRLDEAQRVCDSVAIIKSGKILACGTVEELRKNSGKHSVVVTAKNIS
                     KEIASKVTGDLGRLSRTDGDFLYYELSSEERIPILIQKLVAEGALIYEVRQEKLSLED
                     IFIELVEENNVA"
     gene            510..1217
                     /locus_tag="PK717_07965"
     CDS             510..1217
                     /locus_tag="PK717_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR47629.1"
                     /translation="MWRDIRLVAWKERLQTMANKEIMIGVVILPVIYGIMFPLTAPML
                     SPDKNPALPLLLMLVAALISSIETVYAFAGEKETNTLPTLLTTRLSDSALVLGKSLFA
                     VMLGAISYISTILIYIISYNLLARPAGSPIFLFQDNLPLLLVYLLMPMAFCLFSTSIG
                     MLISTKVKNTKMGAGLSTLPELPFIGLVAWVIFGNPLNIPLMLSYTIIGVGLAILGIA
                     IFLATIGMFSREKMVLR"
     gene            1300..>1812
                     /locus_tag="PK717_07970"
     CDS             1300..>1812
                     /locus_tag="PK717_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase"
                     /protein_id="HOR47630.1"
                     /translation="MNSHAIISFMVESFFQIKNFALKNRIVVPPFVRNLATDDGMATE
                     KLLDSYRQSALSGASIVIVEATYVHELGRIFGFKQLGAISSLHLDSLRQVASSIKSFG
                     IPAIIQLVHSGRMSLNPDCIAPSAIPFENGLTPREMTLDDISFVKECFVNSARMAIEA
                     GFDGLEIHNAH"
BASE COUNT          507 a          436 c          401 g          468 t
ORIGIN      
        1 ggggatgagg caaaaagccg cacttgcacg cgcaatgctc tgcgaacctg aaatcctgtt
       61 tcttgatgag ccaacagcaa accttgatcc ggtctcgtct gccggattca gggacctgat
      121 actggaactg gccagaaaaa gagggacaac ggtttttctc aacacccaca ggctggacga
      181 ggcacaaagg gtctgtgaca gcgttgccat cataaaaagc ggcaagatac ttgcctgcgg
      241 cactgttgag gagctaagga aaaattctgg caaacattcg gttgtagtca cagcaaaaaa
      301 cattagcaaa gaaatcgcat caaaagtcac tggcgacctt ggcaggcttt ccaggactga
      361 tggcgatttt ttgtactacg aacttagttc tgaagagaga ataccaatcc taatccagaa
      421 gctcgtggcc gagggtgcac tgatatatga agtgaggcag gaaaagctct cgcttgaaga
      481 tatctttata gagctggtgg aggaaaacaa tgtggcgtga catcaggctt gtggcgtgga
      541 aggaaagact tcagaccatg gccaacaagg aaataatgat aggtgttgta atactccctg
      601 ttatttatgg gataatgttc ccactgacgg caccgatgct gtccccagac aaaaaccctg
      661 cacttccgct ccttttgatg cttgttgcag cgcttatctc aagtattgaa actgtctatg
      721 cttttgccgg tgaaaaagag accaacacac taccaacgct tctgacaaca agactctctg
      781 actcggcact ggtccttggc aaatcactgt ttgccgttat gcttggtgca atatcctaca
      841 tatcaacaat cctcatatac atcatctcat acaaccttct tgcaagaccg gcaggctcac
      901 caatatttct ctttcaggac aaccttccgc ttcttttggt ttatctgcta atgccaatgg
      961 ctttttgtct gttttccaca tcgataggca tgctgatctc taccaaggtc aaaaacacca
     1021 aaatgggcgc gggtctttcg accctcccgg aactgccatt cataggcctt gtcgcatggg
     1081 ttatttttgg aaacccgctc aatatcccac tgatgctttc ctacacaata atcggcgttg
     1141 gcctcgccat tctgggcatt gccatattcc ttgcaacaat cggcatgttc tcaagggaga
     1201 agatggtcct cagataaaga aaaagcaatt cgccaaaaaa gagacctttt cataaaaaag
     1261 tggaaaggtc tctttttttg ttttccacca cttcaaaaaa tgaactcgca tgctatcatt
     1321 tcttttatgg tagagagctt cttccagata aaaaactttg cactcaaaaa caggatagtt
     1381 gttcctccat ttgtcagaaa tcttgcaaca gatgatggca tggcaacaga aaagttgctt
     1441 gattcataca ggcaaagcgc gctttccggg gcctcgattg tcattgttga agcaacatac
     1501 gtccacgaac taggcagaat attcggtttc aaacagcttg gtgcaatcag cagtttacat
     1561 ctcgacagcc tcagacaagt ggccagttca ataaaatcct ttggcatacc ggcaatcatc
     1621 cagctcgttc attcaggaag gatgagcctg aatccggact gcatcgcccc gtcagccatt
     1681 cccttcgaga acggcctgac accaagggag atgacgcttg atgatatcag ttttgtcaaa
     1741 gagtgctttg taaattctgc cagaatggcc attgaggccg gttttgatgg tctggaaatc
     1801 cacaacgccc at
//
LOCUS       DAOSRI010000323         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_59917_length_1811_cov_4.255695, whole genome shotgun sequence.
ACCESSION   DAOSRI010000323 DAOSRI010000000
VERSION     DAOSRI010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59917_length_1811_cov_4.255695"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PK717_07975"
     CDS             <1..952
                     /locus_tag="PK717_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47631.1"
                     /translation="AQLEYDSEKLQFQKMKVGQFATMDYRCCEAPNSIQSRGRIIFSF
                     ARSFEKYGISSPGIAFSFCFLGRKQGASNISLTLATVKSPDLAVHLLSDLPICRTNID
                     KNGFSLQTSIADGQNIKGNIVTVSGSSTKDQVLVVEGQSKKYIPRLTSEGNYSVDIDL
                     DVHSSDVLFKVEDSSYGRSLWVRRTINRTDIRELAFHIDDRKAWNNGNCEELEASPFI
                     INGKTLVPLRYIAEKLGYQTKWLASEQRIDLLKGQDIITMWVGKKNAYINGQPYQLDA
                     TPQIRAGRTFVPLRFVADAINCDVKWDQTTRTAIVRAEIT"
     gene            complement(945..1733)
                     /locus_tag="PK717_07980"
     CDS             complement(945..1733)
                     /locus_tag="PK717_07980"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF016334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOR47632.1"
                     /translation="MTTQRQEDLLQAFLTIITTESDVISVFLSGSLARGTGDDLSDID
                     LTVVTKNSKGDTWFSSLQPTVEGISKVLLPIRTDTAKRCAVFVFDDLVEMTVTVFEES
                     EVKPSPAYSEIKPLHDPLGLSATLKSKSSLLPKKARIEALIATESLFLWGTLAVRKRL
                     LRDNVWDARDALEKLRYLVIRLMNLRDAKLDGYKNIEKTLDAKSIILLSKTASGYDKD
                     AVLSALAACFDLFVRTRDEVFAQFDIKPNEVTTNKVSQTLNSFR"
BASE COUNT          492 a          383 c          424 g          512 t
ORIGIN      
        1 agcacagctt gaatacgata gtgagaaact ccagttccag aagatgaagg tagggcaatt
       61 tgccacgatg gactatcgct gttgtgaagc acccaattcg atccagtcaa gaggtagaat
      121 cattttctca tttgccagaa gttttgaaaa gtatggcatc tcctccccag gaattgcttt
      181 ctccttctgt tttttgggaa ggaagcaagg agcatcaaat atctcattga cacttgccac
      241 tgtcaaatcg ccggatttgg cagtccactt gttgtctgat ttgcctattt gcagaaccaa
      301 tatcgacaag aacggttttt cattgcaaac aagcattgca gatgggcaaa acataaaggg
      361 taatatcgtt actgtttctg gaagctcaac caaggaccag gtgcttgtcg tggaaggcca
      421 gagcaagaaa tatattccaa gactgacctc cgaaggcaac tattctgttg atattgatct
      481 tgatgtgcac tccagcgacg tgctctttaa agtcgaggat tcttcttatg gcagatcgct
      541 ttgggttaga agaacaataa atagaaccga tatcagagag ctggcatttc atattgatga
      601 cagaaaagcc tggaacaatg gaaattgtga agagctcgaa gcgtccccat ttataatcaa
      661 tggaaaaacg cttgtgccat tgaggtatat tgccgaaaag ctgggctacc agaccaaatg
      721 gcttgcatct gagcagagaa tagacctttt gaaagggcaa gacataataa ccatgtgggt
      781 tggcaagaaa aatgcctaca taaacggaca accataccag cttgatgcaa cgccccaaat
      841 cagggcaggt aggacatttg ttccgctccg ttttgttgcg gatgcaatca attgtgatgt
      901 caaatgggac cagacaacaa gaactgccat tgtccgggca gaaattacct gaatgaattg
      961 agtgtttggg atactttatt tgtagttact tcgtttggct tgatgtcaaa ttgtgcaaat
     1021 acctcgtccc tggttctgac aaaaagatcg aagcacgcgg caagagccga tagaaccgcg
     1081 tctttatcat agccagaagc tgtttttgac agcaatatta ttgatttggc atcaagcgtt
     1141 ttctcaatgt ttttgtagcc atcaagcttt gcatcgcgaa ggttcataag cctgatgaca
     1201 agatacctga gtttttccag tgcgtccctg gcgtcccaga cgttgtccct tagaagcctt
     1261 tttcttacgg caagtgtccc ccacaggaaa agcgattctg tggctatcag ggcttctatc
     1321 cttgcttttt tagggagcaa cgaggacttg ctcttgaggg tcgcagagag gcccaatggg
     1381 tcgtgaaggg gtttaatttc tgaatatgca ggtgaaggtt ttacttctga ttcttcaaat
     1441 acggtgacag tcatctcaac gagatcgtca aacacaaaca ctgcacagcg ctttgcagtg
     1501 tccgttctta ttggaagaag caccttgctt atgccttcaa cggttggctg aagggaagaa
     1561 aaccaggtgt ctcccttgct attttttgtg acgactgtta gatcaatgtc gcttaagtca
     1621 tcaccggttc ccctggccag tgatccagac agaaaaactg atatcacgtc actctctgtt
     1681 gttattatag taagaaaggc ctgaagaagg tcctcttgcc tttgggttgt caccttggca
     1741 cctcatagtt tggggcttct tttgttatct ggatgtcatg tggatgtgat tctctcaatc
     1801 cagcctgtgt t
//
LOCUS       DAOSRI010000324         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_60836_length_1792_cov_3.513529, whole genome shotgun sequence.
ACCESSION   DAOSRI010000324 DAOSRI010000000
VERSION     DAOSRI010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60836_length_1792_cov_3.513529"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK717_07985"
     CDS             complement(<1..483)
                     /locus_tag="PK717_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOR47633.1"
                     /translation="MSKPRIAFFDFSCCEGCQLQVLNCEDELLDLLALVDIVQFREAM
                     TEADWNFDIAFVEGSITREQDEPRLKKIREAAKLVIAIGACSGMAGINAIRNAMPIDK
                     AKEIVYGNKKDAFESTFARPIDTVIKVDGYIPGCPIDRKDFLRTVQALLVGKKPPDHD
                     Y"
     gene            complement(480..1307)
                     /locus_tag="PK717_07990"
     CDS             complement(480..1307)
                     /locus_tag="PK717_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOR47634.1"
                     /translation="MQSLNLYEPMIATIKSVKELTPTEKMFEVELPDGKRLGHGPGQF
                     VEMTVFGVGEAPISICSSPTDGNNFHLTIRKVGDVTGVVHTMKPGDKLGIRGPFGYGF
                     PVDQLKGHDIIIVAGGIGLAPLRSVINYIFHNRSDFGQFTIIHGAKTPSDMLFKDELE
                     GWRKNPNVTYLETVDRPTPEWKGNAGVITTLFKKVGKINPDNTYALVCGPPVMYKYVI
                     LECGVLGIPDSHILASLERRMKCGLGKCGHCQINGLYCCQHGPVFTYTQIKHLEEAL"
     gene            complement(1295..>1792)
                     /locus_tag="PK717_07995"
     CDS             complement(1295..>1792)
                     /locus_tag="PK717_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR47635.1"
                     /translation="KPASSTHLKKQDELLEAKKKLFTAKFTGPAQLLPLELEGQWEAP
                     VWEDMGKKCLSCGQCVLVCPTCYCFDVTDELALSMKKGDRVRKWDGCQLAGFAYVGSG
                     ENFREEPGARVRHRYHRKFKYHVDSHSMTFCTGCGRCWVHCPVEINLVEISNRIFKRE
                     AECKA"
BASE COUNT          402 a          441 c          473 g          476 t
ORIGIN      
        1 ataatcgtgg tccggtggct ttttgccaac caggagggcc tggactgtgc gcaggaagtc
       61 tttgcggtca atcggacagc ctgggatgta gccatcaacc ttgatcactg tgtcaattgg
      121 gcgggcaaag gtagattcaa aagcatcttt cttgttgcca tagactatct cttttgcttt
      181 gtcaatcggc attgcgttcc tgatggcgtt gattccggcc attcctgaac aggctccgat
      241 ggcgatgacg agctttgccg cttccctgat ttttttgagc cttggctcat cctgttccct
      301 ggttatggaa ccctcgacga atgcgatgtc aaagttccag tctgcctctg tcattgcctc
      361 gcggaactgg acaatgtcga cgagggcgag aagatcaagg agctcatctt cgcaattcag
      421 gacctggagc tggcagcctt cgcagcatga aaagtcaaaa aaagctatcc tgggtttgct
      481 catagggcct cctcaaggtg cttgatttga gtatatgtga agacaggtcc gtgctggcag
      541 caatagaggc cattgatctg gcaatgacca catttgccaa ggccgcactt cattcttctt
      601 tcaagagatg caagaatgtg gctgtccggg atgccaagga cgccgcattc gaggatgacg
      661 tacttgtaca ttaccggcgg gccacaaacg agcgcatagg tgttatcagg gttgatcttg
      721 ccgactttct tgaagagtgt tgtgatgacg ccggcattgc ccttccactc cggggtgggc
      781 ctgtcaactg tttcaaggta ggtaacattg ggatttttac gccagccttc aagttcatcc
      841 ttgaagagca tgtccgacgg cgtttttgcg ccgtggatta ttgtgaactg gccaaaatcg
      901 gagcgattgt ggaagatgta gttaatgaca gaacggagcg gggcaagccc gattccaccc
      961 gcaactatta tgatgtcgtg gcctttgagc tggtccacag ggaaaccata gccaaacggt
     1021 cccctgattc cgagcttgtc accaggtttc atggtgtgga caacgccggt gacgtctcca
     1081 accttgcgga tggtgagatg gaaattgttt ccatcagttg gagaagaaca aatggagatt
     1141 ggagcttcgc caacaccaaa aaccgtcatt tctacaaact gtcccgggcc atggccaagg
     1201 cgctttccat ccgggagctc gacctcaaac attttctcgg ttggcgtgag ctctttcacg
     1261 gacttgattg ttgcgatcat tggttcataa aggttcaggc tttgcattcg gcctcccttt
     1321 tgaatatcct gttggaaatt tcaacgagat tgatttctac tgggcagtgg acccagcacc
     1381 ttccacaacc ggtgcagaag gtcattgagt gtgagtcaac atgatatttg aatttcctgt
     1441 ggtagcggtg gcggaccctt gcgcctggct cttccctgaa gttctctcca gagccaacat
     1501 aggcaaaacc tgcaagctgg caaccatccc atttcctcac cctatcaccc tttttcattg
     1561 aaagggcgag ttcatcagtg acatcgaagc aatagcatgt cggacagaca aggacgcact
     1621 gcccgcagga aaggcatttt ttgcccatgt cttcccaaac tggtgcttcc cactggcctt
     1681 cgagctcaag cggaaggagc tgagcagggc cagtgaactt tgccgtaaag agtttctttt
     1741 ttgcttcaag aagctcatcc tgttttttaa ggtgggtgct ggaggctggt tt
//
LOCUS       DAOSRI010000325         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_61285_length_1783_cov_3.191551, whole genome shotgun sequence.
ACCESSION   DAOSRI010000325 DAOSRI010000000
VERSION     DAOSRI010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61285_length_1783_cov_3.191551"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK717_08000"
     CDS             complement(<1..236)
                     /locus_tag="PK717_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47636.1"
                     /translation="MADLADLERLLTDLRTLPKEEKIDEDLFSICGFPHYERVASNVL
                     AFFLDNKREHGLGDLFMQSLLSCGGIAAEGLDLN"
     gene            complement(263..544)
                     /locus_tag="PK717_08005"
     CDS             complement(263..544)
                     /locus_tag="PK717_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47637.1"
                     /translation="MKIEGKEAIQMRDINQRILELWQTTSKEYLNGLMPMLIDTPNSN
                     QVTFIGLNPSFSEEGFSTYLQKTDDYCDIDVREFYAFPVSVSFNTKKES"
     gene            complement(534..902)
                     /locus_tag="PK717_08010"
     CDS             complement(534..902)
                     /locus_tag="PK717_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47638.1"
                     /translation="MWRTYSADLVKQAHKATIFHEKDILASSVCTCFYCGYQFDPHKE
                     VDLEWWDETNPKGKTLVCPKCGIDCVIGDASGFPITDTDFIKACTVVWFGGYSRISDG
                     QHIIEIKSILHNLKETGIED"
     gene            complement(904..1410)
                     /locus_tag="PK717_08015"
     CDS             complement(904..1410)
                     /locus_tag="PK717_08015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR47639.1"
                     /translation="MKRHENTANYSILKRGYRKLRPIQPYHGAGILFWHEDEDKKLSV
                     LLGLRKYNPQKGMWSIPGGKWEAEDSYDDNMKPNYKAAAIREPWREIRVRVENPENLT
                     FLWSMHVPFYHFEVYACGLPEKRAITPYMELSELKWFPVDSLPEESVGFVRLQVAALI
                     RAGRKGDI"
     gene            complement(1415..1687)
                     /locus_tag="PK717_08020"
     CDS             complement(1415..1687)
                     /locus_tag="PK717_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47640.1"
                     /translation="MERKTAWTEIQQERTRDVLERVDIMAFSFSLSGKPKKGCPLNHV
                     SGRDGYITINDALGGDWRVYEDETDELLKTYNSVDDVVNDGWKVST"
BASE COUNT          460 a          454 c          326 g          543 t
ORIGIN      
        1 taattcagat cgagcccttc ggcagcaatc cctccgcaag aaagaaggct ttgcatgaac
       61 aaatccccca aaccatgttc acgtttgtta tccaagaaaa atgccaggac attggatgca
      121 acccgttcat aatgagggaa tccacaaatg gaaaataaat cctcatcaat tttctcttcc
      181 tttggtaacg ttcttaagtc tgttaacaat ctttccaaat ctgccaagtc tgccatcaga
      241 tacactcctt cactaaaaat tgttaggatt ccttttttgt attgaaggaa acagataccg
      301 ggaatgcata aaattctctg acatcgatat cacaatagtc atcagttttt tgaaggtaag
      361 tactgaaccc ttcctcagaa aacgagggat tcaacccaat aaatgtaact tgattgctat
      421 tcggagtatc aatgagcatc ggcatcaatc catttagata ctctttgctc gtggtttgcc
      481 aaagttccaa gattctctga ttaatatctc tcatttgaat cgcttctttt ccttcaatct
      541 tcaataccag tttccttcag attatgcagg atactcttta tctcaatgat atgttggcca
      601 tcacttatcc tgctatatcc tccgaaccag accacagtac aagcttttat gaaatcagta
      661 tcagttatag ggaaaccaga ggcatcccca atgacacagt cgatgccaca tttcggacat
      721 acgagagtct ttcctttggg attggtctcg tcccaccact caaggtctac ttccttgtgt
      781 ggatcaaact gatagccgca atagaagcag gtgcaaaccg agcttgcaag gatgtctttc
      841 tcatgaaaga tggtcgcttt gtgtgcttgt ttgacgagat cagctgaata cgttctccac
      901 atcctatata tccccttttc gacctgcccg tatcagggca gcaacctgta atctcacgaa
      961 cccgacactt tcttcgggca gtgagtcgac cggaaaccat ttcaactcag ataattccat
     1021 gtatggagta atggcacgct tttcaggaag cccacaggca tatacctcaa aatgataaaa
     1081 tggtacatgc atactccaga gaaaagtcag attctctgga ttctccactc gcactcgtat
     1141 ttcccgccaa ggctcgcgta tcgctgcagc cttgtaattc ggcttcatgt tgtcatcata
     1201 cgaatcctcg gcttcccatt tgcccccggg aatcgaccac atcccttttt gcggattgta
     1261 ttttcttaac cccagcaata cggacaattt tttgtcctca tcctcgtgcc aaaatagaat
     1321 accggctcca tgatagggct ggatgggcct caatttgcga taccctcttt tcagtatgga
     1381 ataatttgca gtgttttcat gtcttttcat actctcacgt actcactttc caaccatcat
     1441 tgactacatc gtcgacagaa ttgtaggtct ttagcagctc atccgtctca tcctcgtaca
     1501 cccgccaatc tccgccgagg gcatcgttga ttgtaatgta tccatccctt ccactgacat
     1561 ggttgagagg acagcctttc ttcggtttcc cactcagact gaaggagaaa gccataatgt
     1621 ctaccctttc caagacatcc ctggttctct cttgttggat ttctgtccat gcggttttcc
     1681 tttccatttc accctcctgg gttaattttt atatgctttt aatcactgct cgagaatcgc
     1741 ttggatgtca ggtttggtat gtgtgatcag cttgctcaat cgg
//
LOCUS       DAOSRI010000326         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_61453_length_1780_cov_4.884638, whole genome shotgun sequence.
ACCESSION   DAOSRI010000326 DAOSRI010000000
VERSION     DAOSRI010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61453_length_1780_cov_4.884638"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PK717_08025"
     CDS             complement(<1..1479)
                     /locus_tag="PK717_08025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOR47641.1"
                     /translation="MDAQLKVYKYFVTPFLVVCTVLLSGLTIYKYMEESYVSFNTGDE
                     FYSWANDNILQVKTWPSFSKDDVKQIADFSFVPQGESKEILGNKPKPAASVCVVKDGD
                     TLFKAGYGSTSADKNVPVDPDKTIFKVASVSKVFTALAVIKLVEEGKLDLQEDINTYL
                     KKTGIVIPDYPVGNNIEPETKEVVLKYLNDSKSKENAPEIYLPETSKNQIRIVHGTEY
                     KWEGFFKHDLLEVYDKLAKSDGRAALQTITPQTSKQAVIEFINKDFPDQWIVFGEEKG
                     NKNAPSPTEHRIVARVIKIRNGYEYIENRQTVRPTPYITLAQLLTHTPGLEKKLTTIL
                     TKNADKIPTLPEYFKQNPPNHIMGSGKLYDYSNYGIALAGLIIEEVSGMPFEEYVKKN
                     VFEKLGMNYTTFEQPIPDELQKNYAKPREWKSEGYYDTKNEFKEFDPYYFNDPPADAM
                     VTTAGDMGKFLRDLFKYEKFFSEDSAYLFTSIAFEQDAVFYPK"
BASE COUNT          437 a          359 c          363 g          621 t
ORIGIN      
        1 ttttgggtag aaaacagcgt cttgctcaaa tgcaatgctt gtaaacaggt aagcggagtc
       61 ttcggagaag aatttttcat atttaaaaag gtctctcagg aattttccca tatcaccggc
      121 tgttgttacc attgcatctg ccggtgggtc attgaagtaa taagggtcga attctttaaa
      181 ttcattctta gtatcgtaat agccttcgga tttccattcc cttggttttg catagttttt
      241 ttggagttca tcagggattg gctgctcaaa ggtggtatag ttcatgccta atttttcaaa
      301 aacgtttttc ttgacgtatt cttcgaatgg cattccggaa acttcttcaa tgatgaggcc
      361 ggcgagcgcg attccatagt ttgaataatc ataaagtttg ccggaaccca ttatgtggtt
      421 tggcggattt tgcttgaaat attcaggaag agtcggtatt ttgtcggcat tctttgtgag
      481 tattgtcgtc aatttctttt ctagccctgg tgtgtgggtc aggagctggg ccagcgttat
      541 atatggagtc ggcctgactg tttgcctgtt ctctatgtat tcgtagccat ttctgatttt
      601 aattaccctg gcaacaattc ggtgctcagt tggtgatgga gcatttttgt ttcctttttc
      661 ttccccaaat acgatccact ggtctggaaa atctttgttt ataaattcta taactgcctg
      721 ttttgatgtt tgtggtgtta tggtttggag ggctgccctg ccatcgcttt ttgcaagttt
      781 gtcataaact tctagcaggt catgtttaaa aaatccttcc catttatatt ctgttccatg
      841 cacaattctg atctgatttt ttgatgtttc tggcaaatat atttctggag cgttttcttt
      901 ggatttactg tcattcaggt atttcagaac aacttctttg gtttctggct cgatattgtt
      961 gccaactgga taatcaggaa tgacaatgcc agtctttttg aggtatgtat tgatgtcttc
     1021 ctggagatcg agtttaccct cttcgacgag ttttataacg gcaagagcag taaaaacttt
     1081 tgaaactgaa gccactttga atattgtttt atcgggatca acaggaacat tcttgtctgc
     1141 gcttgtggaa ccataacctg ccttgaataa tgtgtcccca tctttcacaa cacatactga
     1201 tgcggcaggt tttggtttgt tgccaagaat ttctttggat tccccttgcg gcacaaacga
     1261 aaaatctgca atctgcttca cgtcatcttt tgagaaactc ggccaggttt ttacctgaag
     1321 tatgttgtcg tttgcccagc tataaaattc atctcccgtg ttgaagctga cataggattc
     1381 ttccatatat ttgtatatgg ttaagcctga gagcagaacc gtgcagacaa caagaaaagg
     1441 tgtgacaaaa tacttgtata ctttgagttg tgcgtccata ttccctccat ttagaacgtt
     1501 ttgtttaatg ctattggccc tctggagttt tttcaatagc agccaaaggc ataatcaaag
     1561 atgggaatta ccaaaaactg cttagtaaaa tgcacactaa atgaattaat ttcagtaatt
     1621 attccccaat taacataaaa taaaaaaacc gaacagatta tctgttcggt ttttttatta
     1681 gtccagttat ttggctggtg ctacttgccg cctttgccct ttcccttgcc ttttccacca
     1741 ccacggcacc caccgccgcc gcaaccaccg ccactgcaac
//
LOCUS       DAOSRI010000327         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_61458_length_1780_cov_3.645797, whole genome shotgun sequence.
ACCESSION   DAOSRI010000327 DAOSRI010000000
VERSION     DAOSRI010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61458_length_1780_cov_3.645797"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK717_08030"
     CDS             <1..518
                     /locus_tag="PK717_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOR47642.1"
                     /translation="PLAIEIIDITEGPLMTTDLKRSAFVRTVDSNIGIAHVASTREQG
                     AGPISGTGVFCRVQVKGTNITQETRVRIANIKVFDCNNGFVSVRTMTGTEPTLKLTVS
                     GFLPGDVNGDGKVDTQDLTLLGKTFGLSRGDPNFDARADFNEDGIVDGMDLIILCMNW
                     GATLGDESATP"
     gene            complement(602..1351)
                     /locus_tag="PK717_08035"
     CDS             complement(602..1351)
                     /locus_tag="PK717_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOR47643.1"
                     /translation="MDLTKSAGLEVYFDPENGELIFPQIPDFSGPAIRKLDDARDFLM
                     DPQSTGPDVLYWMYRGVSLPQDREKIESANLRYDITVLSPGKIGKEYVKTIGHYHPKT
                     PGGEAYPEIYEVLFGNALFFLQDWNMGDAVVIEADRGAQILIPPGYGHVTVNPSKDFL
                     VTANVISSKFTSEYDIFREHHGGCYYCVEGGNEKIWVVNPSYYKHPPLRFLGPTEMPV
                     LNGLFGSLYESLAKDPKIFVCMNIPEMCPAF"
     gene            complement(1372..>1780)
                     /locus_tag="PK717_08040"
     CDS             complement(1372..>1780)
                     /locus_tag="PK717_08040"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOR47644.1"
                     /translation="TIIAPRWPSIVGPAVARHTEPYGLLKGSLLVRCDSSTWSAELAN
                     HKSLIVENITKILGKDFVNEIKITIAKSQPNKKIAPKEPEYSKIALSDQEVAMVEDVS
                     GMVPKDLKKDFGEAMASYIIRKKSFSKEDFFNN"
BASE COUNT          486 a          427 c          400 g          467 t
ORIGIN      
        1 atccacttgc aatcgagatc atcgacatca ccgagggtcc acttatgaca actgacctga
       61 agagatcagc attcgtgagg acagtcgatt cgaacatagg catcgcccat gttgcttcaa
      121 caagggaaca gggtgcagga ccaatatcag gcaccggcgt tttctgcagg gtgcaagtca
      181 agggaacaaa catcacccag gaaaccaggg tcaggattgc caatataaag gtttttgact
      241 gcaacaacgg gtttgtctcg gtgaggacaa tgacagggac cgaaccaacg ctcaaactga
      301 cagtgtctgg cttcctccca ggagacgtaa acggcgatgg aaaggtcgac actcaggacc
      361 tcacgctcct tggcaagaca tttgggctct caagaggaga tccgaacttt gacgcaaggg
      421 cagatttcaa cgaggatgga atcgttgatg gaatggacct catcatactg tgcatgaatt
      481 ggggcgccac ccttggcgac gagagcgcaa ccccctgata caaaacaaca aactagccaa
      541 taacaaaaaa gccatccccc aaagggatgg cttttttgtt tggtcttcca aacgcaaagt
      601 atcagaatgc cggacacatc tccggtatgt tcatgcaaac aaaaattttg gggtcttttg
      661 cgaggctctc gtagagactg ccaaaaagac catttagcac aggcatttct gtcgggccga
      721 gaaacctgag aggggggtgt ttgtagtagg aaggatttac aacccatatc ttttcgttcc
      781 caccttcaac acaataatag caaccaccgt ggtgttctct gaagatatca tattcagagg
      841 taaacttgct tgagatgaca ttggctgtta caagaaaatc ttttgatgga ttgactgtga
      901 cgtgaccata tcctggcggg ataaggatct gtgcgcccct gtcagcttca atcacaacgg
      961 catcacccat gttccaatcc tgcaaaaaaa acagggcatt tccaaagagg acttcataga
     1021 tttcaggata ggcctcgcca ccaggtgttt ttggatggta gtggccaatt gtcttcacat
     1081 actcttttcc aattttcccg ggtgagagga cggttatatc gtatctcaaa ttggccgatt
     1141 caattttctc cctgtcctgg ggcaaagaaa cacccctgta catccagtag agcacgtcag
     1201 gcccagtact ttgagggtcc ataaggaaat cccttgcatc gtcaagtttt ctgatggccg
     1261 ggccagaaaa atctggtatc tgcgggaaaa taagctcccc gttctccgga tcaaaataaa
     1321 cttcaagtcc tgctgatttc gtcaaatcca tagtttcaca ctcccaaaaa ttcagttatt
     1381 aaaaaaatct tcttttgaaa aagatttctt tcttatgata tacgaagcca tagcttcgcc
     1441 aaaatctttc ttcaggtctt ttggcaccat gccactcaca tcttcaacca tcgccacttc
     1501 ctgatcagac agggcaattt ttgaatactc cggttctttt ggagcgattt ttttatttgg
     1561 ctgcgatttg gcaattgtta tctttatctc attgacaaag tcttttccaa gtatttttgt
     1621 tatgttctcg acaataaggc tcttgtggtt ggcaagctcc gcgctccagg tggaactgtc
     1681 gcacctcacc aggagacttc ctttgagcaa tccataaggc tcggtgtgtc ttgcaacagc
     1741 ggggccaaca attgatggcc atctcggtgc aattatcgtt
//
LOCUS       DAOSRI010000328         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_62249_length_1765_cov_2.500585, whole genome shotgun sequence.
ACCESSION   DAOSRI010000328 DAOSRI010000000
VERSION     DAOSRI010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62249_length_1765_cov_2.500585"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PK717_08045"
     CDS             complement(<1..1056)
                     /locus_tag="PK717_08045"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR47645.1"
                     /translation="MKTLLTGLTKRNDCDYLEIRIEDTDHTGISYQGHDLDQVQRTKA
                     FGGAIRALYKGGWGFASFNSIDELESKVEAAVQMARTIGNTINEKSMLAPTKSIVDTV
                     KLDVKNDVRNVPLGKKVDLLKGYTELMLSVSPLVKTTRVYYKDAYSKLYFANSEGAYI
                     EQEKMDLGANFIAMSAKDGVSQNYFTGVGSSDDFNVVLDLDQKVLEVSETAVKLLDCE
                     QVNSGSYTVILDPILSGTFIHEAFGHLSEGDNVYENPSLAEVMTMGRQFGKPILNVYD
                     TGLDKGTRGYLKYDDEGTPTEKTYLIKEGKLVGRLHSRETAGKMGEKATGSARAINYK
                     FAPIPRMRNTCIEIGNSS"
     gene            complement(1145..1294)
                     /locus_tag="PK717_08050"
     CDS             complement(1145..1294)
                     /locus_tag="PK717_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47646.1"
                     /translation="MFEQRSKMLDFFFKFSLALLITITLAIALWMVALGGTKWLSYWV
                     SQILK"
     gene            complement(1287..>1765)
                     /locus_tag="PK717_08055"
     CDS             complement(1287..>1765)
                     /locus_tag="PK717_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR47647.1"
                     /translation="VEINGTALEELDGQAWRGKIGFLSQHPYFGPGNLVENVTFGRET
                     KNDPVEVIKLCRLSGVAERLGDGPLGARARNISAGEKQRVALARVMLMDREILLLDEP
                     LAQVDIPTAKEIFADISPVLRKGTTIIITHNPVLIKMADKVYFIYDGKAFEGGPNV"
BASE COUNT          431 a          435 c          358 g          541 t
ORIGIN      
        1 agaggaattg ccaatctcga tgcaggtgtt gcgcatcctt ggaattgggg cgaacttata
       61 gtttattgcc cttgctgaac cagtggcttt ctcacccatt tttccggccg tttctctgct
      121 atggagtctt ccaacaagct tgccttcctt gataaggtaa gtcttctctg tcggtgttcc
      181 ctcatcatcg tatttgaggt atccccttgt tcccttgtcc agacctgtat cgtagacatt
      241 caagattggt tttccgaatt gcctgcccat agtcataact tctgcaagtg aggggttttc
      301 gtaaacatta tcaccctcgg agaggtgtcc aaaggcctca tggataaatg taccggagag
      361 aattgggtca agaattacag tgtaggaacc actgttgact tgctcacagt caagcaactt
      421 gactgctgtc tcagagactt caagcacttt ctggtcaaga tcaagaacaa cgttgaagtc
      481 atcactggag ccaacgccag taaagtaatt ttgtgatacc ccgtcttttg cagacattgc
      541 gatgaagttg gcacccagat ccatcttttc ctgttcgata taagcgccct ctgagtttgc
      601 aaaatataat tttgagtatg cgtccttgta gtagaccctt gtggttttga ctaatggtga
      661 aacagaaagc atcaattctg tataaccctt tagcagatca actttttttc caagcggtac
      721 atttcttaca tcattcttta cgtctagctt taccgtatca acgattgatt ttgtaggcgc
      781 aagcatgctt ttttcattaa tagtgttgcc aatagtacgc gccatctgca cagctgcttc
      841 aaccttggat tccaattcat caattgaatt gaaggaagca aaaccccaac cacccttgta
      901 aagtgccctg attgcaccac caaaggcctt tgtcctctgg acctggtcaa ggtcatggcc
      961 ttgataggaa ataccagtgt ggtcagtgtc ttcaatcctt atttcaaggt agtcacagtc
     1021 atttcttttt gtcaaaccgg tcaaaagtgt tttcattgcc acctcggaat tttctttgta
     1081 acaacaaagt tattactttg gacccaaaac ttcaagcaaa gttttgactg cggttttggt
     1141 tttgttattt aagaatttgc gacacccaat atgaaagcca cttggtccct ccaagggcca
     1201 ccatccacag agcaattgct agtgttatcg tgatcaaaag ggcaagcgag aacttgaaga
     1261 aaaaatcaag catctttgat ctctgctcaa acattgggcc ctccttcaaa tgctttgcca
     1321 tcatagatga agtacacctt gtctgccatt tttatcagta ctggattgtg cgttataatg
     1381 attgtggttc ctttcctcag cactggggat atgtctgcaa atatctcctt ggccgttggg
     1441 atgtctacct gtgccagagg ttcatccagc aggagtatct ccctgtccat cagcatgact
     1501 cttgccaagg caactctctg tttctcgcca gcggatatat ttcttgcacg tgcccccaac
     1561 gggccgtcac caagccgttc agctaccccc gaaagcctgc agagctttat cacttccacc
     1621 gggtcatttt ttgtttctcg cccaaaagtg acattttcga ccaggttgcc agggccaaaa
     1681 tatgggtgct ggcttagaaa acctatcttt cccctccagg cttgtccatc cagctcctcc
     1741 aatgcagtgc cgtttatttc cacat
//
LOCUS       DAOSRI010000329         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_62378_length_1762_cov_5.451084, whole genome shotgun sequence.
ACCESSION   DAOSRI010000329 DAOSRI010000000
VERSION     DAOSRI010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62378_length_1762_cov_5.451084"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1379)
                     /gene="rseP"
                     /locus_tag="PK717_08060"
     CDS             complement(63..1379)
                     /gene="rseP"
                     /locus_tag="PK717_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOR47648.1"
                     /translation="MSTILNIIYALLILDVLVIAHEFGHLFAARSIGVPALSFSIGMG
                     PVIARKKFKYFGDTEFRISLFPVGGYVMVSGEAEDSDDPNSLYRKSPWQRAWFSVSGA
                     LTNFVFAFIVFVVVALAFGDPVYLFNRIGTVDPNMPAAKAGIQSGDWILEIDGTQTPT
                     WDAVVAEIRTRPDKEVPVLVGRESGKAEAEVSPDTSIWVTGSQVAKLSEFKFPEEYPS
                     DDIFIGIEGKYFRLNSIELVGNEEAGNWNKTKELLKAITSTSKITFGSSLETQTVKVT
                     IAGVEEEQENGLIKVGKLGITASQGVKYKRYNIVEAIWISLEKFWLFLVAFFNFFVGL
                     FSGKGVGDVAGPVGIIKITGQMASMGWAVILGFAAMLSMNLGFLNLIPFPGLDGARFL
                     FSVVEGITGKKLPRAVENVIHAVGFIILIGLILLVTANDLFGIFRK"
     gene            complement(1364..>1762)
                     /locus_tag="PK717_08065"
     CDS             complement(1364..>1762)
                     /locus_tag="PK717_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47649.1"
                     /translation="AKLQASPPDMRYPISYALHFPSRTVSDLDGLKFPANWSFGELTG
                     DASKSVALAKSAFSKGPPWLVAYTAADEAAIELFLSKSLSFGLIPEVISKTMSRIENI
                     DLSSPQKITSHYTNCRQIALEIGKKLCQQY"
BASE COUNT          474 a          452 c          386 g          450 t
ORIGIN      
        1 cgtctggacg gcaatgggcg caccaccacc aatcctgacg ttgccaacca agacgctcct
       61 gctcattttc tgaatatccc gaacaggtcg tttgcagtca caagcagtat caatcctatc
      121 aatattataa aaccgactgc atggatgaca ttttcaacgg cccttggcaa cttcttgcca
      181 gtgataccct cgacaacaga gaacaggaac ctggcaccat caagcccggg gaacggaata
      241 aggttcagga acccaagatt catggacaac atcgcggcaa aaccgagtat gacggcccag
      301 cccatgctgg ccatctggcc tgttattttt atgatgccaa caggaccggc aacgtcgccg
      361 acgcccttgc ccgaaaacag gccaacaaag aagttgaaga acgcaacgag gaaaagccag
      421 aatttttcca gcgaaatcca tatcgcctca acaatgttgt atcttttgta tttgactccc
      481 tgcgatgctg ttatcccaag ctttccaacc ttgatcaagc cgttttcctg ctcttcttca
      541 acacctgcga tggttacttt gacagtctgg gtttcaagtg acgatccaaa tgtaattttg
      601 cttgttgaag tgatcgcctt caggagttct tttgtcttgt tccagtttcc tgcctcttca
      661 ttcccaacca attcaattga attgagcctg aaatatttgc cttctattcc aataaaaata
      721 tcatcgcttg gatattcttc cgggaatttg aactctgaaa gcttggccac ctggctacct
      781 gttacccata ttgatgtgtc tggagaaact tctgcttcgg ccttgccaga ttcccttcca
      841 acaagaactg gtacttcctt gtctggcctt gtccttattt cggccacaac cgcatcccag
      901 gtgggggttt gtgtgccatc tatttcaagt atccagtcac cagactgaat gcctgccttt
      961 gcggccggca tgtttggatc gactgtgcct atcctattga aaagatatac cggatcacca
     1021 aaagccagcg ccacaactac aaagacaata aaagcaaaaa caaaatttgt cagggcacct
     1081 gaaacagaaa accatgccct ctgccagggg ctttttctat agagactgtt tgggtcatct
     1141 gagtcttcgg cttcgccgga aaccatgaca tagccgccaa cagggaaaag cgatatcctg
     1201 aactctgtat caccaaagta tttgaatttt tttcttgcaa tgaccgggcc catgccgatt
     1261 gaaaaactca gcgccggcac accaattgac ctggcggcaa agaggtggcc gaattcatgg
     1321 gcaatcacaa gaacatcaag aatcaaaagc gcatatatta tgtttagtat tgttgacata
     1381 gcttttttcc tatctccaac gcaatctgcc tgcaattggt gtagtgggag gtgatctttt
     1441 gggggctgga aagatcaata ttttcaattc ggctcatggt cttggaaatg acttccggga
     1501 tcaggccaaa agacaaagac ttcgaaagga agagttcaat tgccgcctca tcagccgcag
     1561 tgtaggcaac cagccatggt ggcccctttg aaaaagccga ttttgcaagg gcaaccgact
     1621 ttgaggcatc gccggtcaac tcaccaaatg accagtttgc cggaaatttg agcccatcaa
     1681 gatcagacac tgtcctactc ggaaaatgga gggcatagga tatcgggtac cgcatgtctg
     1741 gcggagaggc ctgcaattta gc
//
LOCUS       DAOSRI010000330         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_62561_length_1759_cov_3.112089, whole genome shotgun sequence.
ACCESSION   DAOSRI010000330 DAOSRI010000000
VERSION     DAOSRI010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62561_length_1759_cov_3.112089"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1759)
                     /locus_tag="PK717_08070"
     CDS             complement(<1..>1759)
                     /locus_tag="PK717_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOR47650.1"
                     /translation="TMDIYVNGIKKGELLPQKELDDIERLEFPHITRHNPSMLIYSMK
                     APDSPLKNKVVTIQHSQPDPMVGKLQDVASFISGKVQGGYKIVIATKQPGRVRELIEL
                     QDGLVPSKPEKLDEESAPFFDIVNMSLERGFEDRRRKVLLLTDNELFGWRRRIGATKR
                     YRGGSPIKSVDELHPGDLLVHLTFGIGIYRGLKTVTAAGITKEYLQVEYAKGDKLYIP
                     PERLPLIHKYLGNPETVTINKLGGREWPEALARVARGTKDIAAELIRLYARREMSKGY
                     SFEEDTIWQQEMESAFPYEETTDQLKAIMEVKRDLERSKPMDRLVCGDVGYGKTEVAI
                     RAAFKVIVAGKQVAILVPTTILALQHYNTFKERFAPFPIKVEQLSRFKGKTSQKKVVE
                     GITDGSIEMVIGTHRLLQSDIKFKDLGLLIIDEEQRFGVEDKEKIKRLKENIDVLTLT
                     ATPIPRTLEMALTGVREISQINSSPEGRKPVKTYVQAYSETAAKEAIEIELARGGQVY
                     YVNNRIQGLGSIMWKLKKIVPNARIAIAHGQMDSDELEDIMLSFYKGEIDVLLHNNHR
                     IGPGYPNCKHNNRRKRTKP"
BASE COUNT          354 a          423 c          402 g          580 t
ORIGIN      
        1 caaggttttg tgcgttttct acgattattg tgtttgcagt tgggatatcc aggcccgatt
       61 ctatgattgt tgtgcaggag cacatcgatc tcgcccttgt agaagctgag cattatgtcc
      121 tcaagctcgt cgctatccat ctgcccgtgt gctatggcaa tccttgcatt gggtacaatc
      181 ttcttgagct tccacatgat ggaaccgagg ccctgtattc tgttgttgac gtaatagacc
      241 tggccgcccc ttgcaagctc gatttcaatg gcttcttttg cggcagtttc cgagtatgcc
      301 tgaacgtatg tcttgactgg ttttctgcct tctggagaag aattgatctg cgaaatctcc
      361 ctcactccag tcagcgccat ttcgagcgtc cttgggattg gggttgcggt gagggttaag
      421 acatcaatat tttctttgag tcttttgatc ttctctttgt cctcgacgcc aaacctttgc
      481 tcttcgtcaa ttattagcaa ccccaaatcc ttgaatttga tgtcactttg caggaggcga
      541 tgggtgccaa tgaccatttc gattgatcca tcggtgatgc cttccacaac ttttttctgg
      601 ctggttttcc ccttaaatcg tgaaagttgc tccactttta tcgggaacgg ggcaaagcgt
      661 tccttgaaag tattgtaatg ttggagtgcg agtattgttg tagggacaag tattgctacc
      721 tgtttcccag ccacgattac cttgaatgcg gcccttattg cgacctcggt ctttccatag
      781 ccaacatcgc cacaaacaag cctgtccatt ggttttgatc gttcgaggtc tcgtttgacc
      841 tccatgatgg ccttgagctg gtcagttgtt tcttcatacg gaaaggccga ctccatttcc
      901 tgctgccaga tggtgtcttc ctcgaatgaa taacctttgg acatctctct tctggcatag
      961 agccttatca actctgcggc aatgtccttt gtccctcttg cgacccttgc gagtgcttct
     1021 ggccattccc ttccgccgag tttgttgatt gttactgttt cggggtttcc aaggtattta
     1081 tggatcagtg gaagtctttc aggtggtatg tagagcttgt ctccctttgc atattctacc
     1141 tggagatatt cttttgtgat tcctgccgca gttacggttt taagccccct gtaaatgcca
     1201 attccaaagg tcagatggac cagaagatcg cctggatgga gttcatcaac agatttgatt
     1261 ggtgagccac ccctgtatct ttttgtggcg ccaatccttc ttctccaccc aaagagctca
     1321 ttgtcagtca ggagcaggac tttccttctt ctgtcctcaa aacccctctc cagtgacatg
     1381 ttgactatat caaagaaagg cgctgattct tcgtccaatt tctccggttt ggagggaaca
     1441 agtccgtcct ggagttctat aagttctctg actctgccag gctgttttgt tgcgatgaca
     1501 atcttgtaac caccttgaac tttgccagat ataaatgatg caacatcctg gagcttcccg
     1561 accattgggt ctggttgcga atgctggatt gtgaccactt tgttttttaa gggtgaatca
     1621 ggggccttca tgctataaat cagcattgat gggttgtgtc tggttatgtg cggaaactcc
     1681 agcctttcga tgtcatcaag ttctttttgc ggtaggagtt cacctttctt gataccattt
     1741 acatagatgt ccatcgtat
//
LOCUS       DAOSRI010000331         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_64542_length_1721_cov_2.888355, whole genome shotgun sequence.
ACCESSION   DAOSRI010000331 DAOSRI010000000
VERSION     DAOSRI010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64542_length_1721_cov_2.888355"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK717_08075"
     CDS             complement(<1..961)
                     /locus_tag="PK717_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOR47651.1"
                     /translation="MKSFTKVFIPYGGYYSTPFCRWQGSMQHDNAVFLGPNTARRWFL
                     EKRKIDPTVIDYLYFGTTVAQHHWFFSHQMAGAIITDDKKFVPGLFIHQACSTSTTIV
                     NLAAQNIELGTYEVCFGLMSDRCSNGPHTTWANPLGPGGEPYREDWNMDNFGGNPRVP
                     VAMVETAENVAREAGITKEECDAVVLRRFEQYQDALANDRAFQKRYMFPALCTVSRKK
                     TKLVEEDEGWTPTTAEGLASLKPVRPGGVHSFGSQTFPADGNCGIIVTTRDKAKELST
                     DPKIEVQVISYGAARVEPAHMPAAPVPAAQMALDMAGLKITDMK"
     gene            complement(1184..>1721)
                     /locus_tag="PK717_08080"
     CDS             complement(1184..>1721)
                     /locus_tag="PK717_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47652.1"
                     /translation="LVEHELIFEKFIDVGFDKRNFLGCISPPPDYKTLASTAVKSSFY
                     RKEIGFPWWCLEYFRHWISIRHWIGDEPALYADCALFWGSKVHPSMKATTRNIRDLIE
                     SGVLTDRKQYHRHVNTLLELMKIPKFTEWTFIKSGKDSTPLVYRLAHKEKINPNGSNS
                     HVLKTMGTTLKHGFQSNL"
BASE COUNT          457 a          379 c          373 g          512 t
ORIGIN      
        1 ctttcatgtc agtgattttc agaccggcca tatcaagggc catttgagcg gcaggaacag
       61 gggcagcagg catatgggca ggttcgactc tggctgctcc gtatgagatt acctgaactt
      121 caatcttggg gtctgtgctt aattcttttg ctttatctct tgtggtcacg attatgccgc
      181 aatttccatc agcagggaaa gtctggctgc caaaactatg gacaccgcca gggcgcaccg
      241 gcttgagtga tgcaagacct tctgcagttg ttggtgtcca gccctcatcc tcttcaacga
      301 gttttgtttt ttttctgctt actgtgcata acgccggaaa catatatctt ttctggaagg
      361 ccctgtcatt tgcaagggca tcctggtact gctcaaaacg ccttaaaacc acggcatcgc
      421 attcctcttt tgttatacca gcttcccttg ccacattttc agctgtttcc accattgcaa
      481 cgggcacgcg cgggtttcca ccgaaattgt ccatattcca atcttcgcga taaggctctc
      541 ctccgggacc aagagggttg gcccaagtcg tgtgagggcc attagagcac cggtcgctca
      601 taagtccgaa acacacctca tacgtgccaa gttctatgtt ctgggcagcg agattgacaa
      661 ttgtcgttga tgttgagcag gcctgatgga taaaaaggcc aggaacaaat tttttgtcat
      721 cggttataat agcccctgcc atctggtgag aaaaaaacca gtgatgctga gcaacagtgg
      781 ttccaaagta aagatagtca ataactgtgg gatcaatctt ccttttctcg agaaaccatc
      841 ttcgggcagt attgggccca agaaaaaccg cattgtcatg ctgcatgctg ccctgccacc
      901 ggcaaaaagg tgttgaatag tacccgccgt aaggtataaa cacctttgta aaagatttca
      961 taaaattacc tcctattttg ttagtttatt gcctttaggt gaattgcata aagtgtctat
     1021 tatggaaaac aataccatct tttgtgtact atagagaaca tcaaatgtca ataaagaaaa
     1081 acttataata tgttaagaaa agttataggt gtgaacaatg tgactgcaat aaataatgtt
     1141 aagctgtgca gccgctacaa gattcaccgt gttttttatt catttaaaga tttgattgaa
     1201 atccatgttt caatgtcgtg cccattgttt tcaaaacatg cgagttacta ccattgggat
     1261 ttattttttc tttgtgtgcc aatcgatata ccagaggggt gctgtctttg ccggacttga
     1321 taaatgtcca ttctgtaaat tttggtattt tcatcagttc cagcagtgtg ttgacatggc
     1381 gatggtactg ttttctgtct gttaacaccc ctgactcaat taaatcccga atgttccggg
     1441 ttgttgcctt catagaggga tgaactttcg atccccagaa cagggcgcaa tctgcataca
     1501 aggcaggttc atcccctatc cagtgacgta ttgaaatcca atgcctgaaa tactccaaac
     1561 accaccaggg gaaaccgatt tcctttctat aaaatgagga tttcacagct gtagaggcca
     1621 gtgttttata gtctggggga ggagaaatgc atccaaggaa atttcttttg tcgaatccaa
     1681 catctatgaa tttttcaaag ataagttcat gttcgaccag a
//
LOCUS       DAOSRI010000332         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_65526_length_1703_cov_4.250607, whole genome shotgun sequence.
ACCESSION   DAOSRI010000332 DAOSRI010000000
VERSION     DAOSRI010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65526_length_1703_cov_4.250607"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1078
                     /locus_tag="PK717_08085"
                     /pseudo
     CDS             335..1078
                     /locus_tag="PK717_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraG/TraD/VirD4 family protein"
     gene            1228..>1703
                     /locus_tag="PK717_08090"
     CDS             1228..>1703
                     /locus_tag="PK717_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOR47653.1"
                     /translation="MNIYALAKKTIAENGGIARNVDFHKNGLNNSQITYLRNRGYLDR
                     VRRGYYQLSSYEDSSEETIIARLFPDGIICMDSALFHYGYSDRTPLEWTIAFPRTITR
                     TRLGVNSPRIKPYFISEEIFLLGKTEQTINDVKLAMYDRERTICDCFKYKRKIDSE"
BASE COUNT          515 a          350 c          373 g          465 t
ORIGIN      
        1 tggtaaccac ataaagtaat ttataaaaca cacaaagaac atctgacttg attaatccaa
       61 agcacaaacc tttctttccg cttaggtttt caataacaca gatcaacttg agagggcaaa
      121 ctgctgtatt tgattacatt tgttattaga ttcttgccga ccatataaaa ctgtcgtttc
      181 attaagatgt gaaagaagtt tatctgtttc acctttattg atatggcctt gataccatgt
      241 aaaagcaggg aagctctttt taataaatgg ggtacactac agtaacaact cgctttaatg
      301 tgattgatga ctaagcactt gacaaatgac gacagggaag accgccaagt cgatcttgat
      361 ctcctgtggt gccaggcttg ctccctttga catttacgaa ttgcgggagc tgatgagcta
      421 cgatgaaatg gatctggatt tgttggggga cagactgtct gcactctttg tgatcatcag
      481 tgacacagat gacaccttca acttcgttgc cgctctgctc tacacccagt tgttcaatct
      541 gctttgtgat aaagctgatg atgtttacgg tggcaggctc cctatccatg tgcgctgtct
      601 tttggatgag ttccccaata ttggtcaaat tcctcgcttt gaacgtttga tcgccacgat
      661 ccgcagtcgt gagatctcag cctgtatcgt tctgcaagcc cagagtcaac taaaggcgct
      721 ctataaagac catgccgaga cgattgtagg taactgtgac agtatgctct ttttgggtgg
      781 aaaagaaagg accacgctca aggaaatctc tgagctattg ggcaaggaaa cgattgacct
      841 ggataacact tcagaaaata gagggcgaga gaaatcacac gggatcagcc atcaaagaat
      901 gggcaaggaa ttaatgaccc aggatgaact ggctgtgctg gacaattcca aatgtatcct
      961 gcagctgcgg ggcgagcgtc cgtttttatc tgataagtat gacattacga atcatcctaa
     1021 ctacagatat ctttcagacg tagatccaaa tagaactttt aatatcgagg aggaaatgaa
     1081 ttccacccct cgaatgggta ctgagagagt aaccagcatt tatgagatgg gcaacttcgt
     1141 tgaatcctga tttgaattat ttgatcgaag aagctatact ctaaccatac acaaatgtaa
     1201 tcacttgtgt atggttagga gactcggatg aatatctatg cactcgcaaa aaaaacaatt
     1261 gccgaaaacg gcggtatcgc aagaaatgtc gattttcata aaaatgggct gaacaattcc
     1321 cagattacct atcttcgtaa caggggttac cttgaccggg tcaggagagg ctattaccaa
     1381 ctaagctcat acgaggattc gtcggaagaa acgattattg cccggctttt cccggatgga
     1441 ataatctgta tggattcggc cctgtttcat tatggatata gtgatcgaac accgttggaa
     1501 tggacaattg ctttccctcg gaccatcaca aggacacgcc taggggttaa ctctccaaga
     1561 ataaaaccat actttatatc ggaagaaatt tttctgcttg gcaaaaccga gcaaaccatc
     1621 aatgatgtca aattggccat gtacgatcga gagcgcacaa tctgtgattg ttttaagtac
     1681 aaaagaaaaa tagacagcga aat
//
LOCUS       DAOSRI010000333         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_66702_length_1682_cov_5.794100, whole genome shotgun sequence.
ACCESSION   DAOSRI010000333 DAOSRI010000000
VERSION     DAOSRI010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66702_length_1682_cov_5.794100"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1476)
                     /locus_tag="PK717_08095"
     CDS             complement(76..1476)
                     /locus_tag="PK717_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47654.1"
                     /translation="MKKLKVFLVILVLVGLIGLVVYLFLIPKKNSDTDIFFNATVKAE
                     WVPYKKKPDIILSKDTTYIGRYWLGDLDYMLLILDNTNHTLNLFSDPTLEKIIKRIPL
                     PGPVLSWRKGNFYVKIKNPENPNDDYNDLHDLYALVCKTESTNKLVTIDCKAGDMLDF
                     KTRSIRNITDMKIYSSFDIGPANRIIDIIIPDPEGRSYHAIDTRVPIEKLVIICQFPD
                     MQTLSYYSTNGHLLNKQVLDRKMKFSINSYGYSNKKAYDGSSILNCIFKGTQLEISSK
                     APLGSTKDISSGTFCAEVKENETINYPSYLRVFYDDEVLYVIAGSFTPLRYEQKELSE
                     STDGSFFALEDKVLFFPQNPKHKEVPTPITVDSGLSRHEPFFSNTDFYHDDETITAFI
                     ETGTSGQRIDAYITNIARHEKFIKIKSDWFQPFDFAINLPMHRIYLYSDKGYYKIDIP
                     TNIFNTARFTENQGDK"
BASE COUNT          476 a          322 c          330 g          554 t
ORIGIN      
        1 cctgttgctc ttgaccacca gaaggccatt gccgtgactg tgtgggggag gtaggaagct
       61 aggctaatcc ataaatcatt tgtcgccctg gttttcggta aaacgagctg tattaaaaat
      121 atttgttggg atgtcaattt tatagtaacc cttgtccgag tatagataaa tcctgtgcat
      181 tggtagattg attgcaaaat caaacggctg gaaccaatcg cttttgattt tgataaattt
      241 ctcgtgcctt gcaatatttg ttatgtatgc atcaattctt tgacctgatg tgcccgtttc
      301 aatgaatgct gtaatagttt catcatcatg gtaaaaatca gtattggaaa agaaaggttc
      361 atggcgggac aatccagaat caacagttat tggtgtggga acctccttgt gtttagggtt
      421 ttgaggaaaa aacaaaacct tatcttcaag agcaaaaaat gatccgtcag ttgattcgct
      481 cagttccttt tgctcatagc gcaatggcgt aaaactccca gctataacat aaagtacctc
      541 atcatcataa aaaaccctaa gatacgatgg atagtttatt gtttcatttt cttttacttc
      601 agcacagaac gtgccactcg atatgtcttt tgtggaacca agaggggcct tactggaaat
      661 ttcaagctgt gtgcctttaa atatgcaatt taaaatggat gaaccatcat aggccttttt
      721 gttgctgtag ccatagctat taatgctgaa tttcattttt ctgtccagga cctgcttgtt
      781 taacaggtgt ccgtttgttg aataatatga aagcgtttgc atgtcgggga actggcaaat
      841 tattaccagt ttttctatgg ggacacgagt atcaattgca tggtaacttc ttccctcagg
      901 atccgggata atgatatcta tgatgcggtt tgctggacct atatcaaaag agctatagat
      961 tttcatatca gtaatgttac gaatgcttct ggtcttaaaa tccaacatat ctccagcttt
     1021 gcagtctatt gtaacaagtt tgtttgtgct ttctgttttg cagacaaggg cataaaggtc
     1081 atgcaaatca ttgtaatcat cattgggatt ttcggggttt ttgattttga cataaaaatt
     1141 acccttgcgc caggacaaga cagggccagg caatggaatt cgttttatga ttttttcaag
     1201 tgttggatca gagaacagat tgagtgtgtg atttgtattg tcgagtatca gcagcatata
     1261 atccaaatct ccgagccaat aacgcccaat atatgttgta tctttagaca atattatatc
     1321 tggtttcttt ttgtatggta cccactctgc cttgactgtg gcattaaaga atatgtctgt
     1381 gtcgctattt ttcttgggaa tcagaaagag gtataccaca agtccaatca accctaccaa
     1441 caccagaatg acaagaaata ccttcaattt cttcatgagc actccacaca aaagtaactg
     1501 tggctatatt tcaattttgt tttgcaattg ttcattttcc ctccatcatt ctaattataa
     1561 acacccaatt atgcaaattt gctccaaaaa ttagtgtcca aaaatgacac tgatacttct
     1621 cactcccact cgatcgtggc tggtggcttg tttgtgatgt caaaaaccac cctgccgatc
     1681 tg
//
LOCUS       DAOSRI010000334         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_67181_length_1674_cov_3.158122, whole genome shotgun sequence.
ACCESSION   DAOSRI010000334 DAOSRI010000000
VERSION     DAOSRI010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67181_length_1674_cov_3.158122"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK717_08100"
     CDS             complement(<1..233)
                     /locus_tag="PK717_08100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOR47655.1"
                     /translation="MNKALIFGVAGQDGSYLAKLLLEKNYEVLGVEKEFIGDLWRFRK
                     VGIEGAKGFNLKECDITDKKACDELLVSFQPDE"
     gene            complement(226..>1674)
                     /locus_tag="PK717_08105"
     CDS             complement(226..>1674)
                     /locus_tag="PK717_08105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR47656.1"
                     /translation="EDLRTALETLWKNDNQREQLCTRAREEILANHSPSKCAQLYFET
                     IETFYDNSKYGIHSLINAIAFIDGYSQSQEELLKLATAIVESQPKKVGLKQLFVDISE
                     LIQRDTKTGIQRVTRSILKEMIFNPPTGYRVEPVYASLEEPGYKYASNFTLKFIDCPN
                     GWLLDSPIDYYRGDVFLGLDLQPNIVPLQQKFLMQIHNSGVKVLFILYDLLPILHAEF
                     FYPEGKEIVTSWINTIMLFDGAVCISKAVADELTNWMSTNVPDRMDKFEIKWFHLGAD
                     IDNSIPTLGIPEGADHILSQISSNKSFIMVGTIEPRKRQDLVLEAFECLWNEGISTNL
                     VIVGKKGWLTDDLCDRIRKHREYGKRLFWLEGISDEYLEKVYAASTCLIAASEGEGFG
                     LPLIEAAQKKIPIIARDIPVFREVAGKHAFYFSATTGDELAGSIKEWMTLYNDDRHPK
                     SDDMPWLTWKESTKQLLKAIGIESEGSGSDDE"
BASE COUNT          446 a          378 c          306 g          544 t
ORIGIN      
        1 atctcgtcgg gctgaaaaga gacgagcagc tcatcacagg cctttttgtc ggtgatgtca
       61 cattctttta ggttaaatcc tttggctcct tctattccga ctttcctgaa acgccaaagg
      121 tccccgataa attccttttc cacgccgagg acttcgtaat ttttttccag gagcagtttt
      181 gcaaggtatg atccatcctg tccggcaact ccaaagatca aagctttatt catcgtcaga
      241 tccacttcct tcactctcaa tccctatggc cttcaggagc tgctttgtgc tctctttcca
      301 tgtcagccac ggcatgtcgt cagatttggg gtgcctgtca tcattataaa gggtcatcca
      361 ttcttttatc gaacccgcaa gttcatcccc ggtagtggca gagaagtaaa atgcatgttt
      421 gccggcgact tcacggaata ccggaatgtc cctcgcaata attggaattt tcttttgtgc
      481 tgcctctatc aggggtagcc cgaaaccctc tccctccgat gccgctataa gacaggtcga
      541 tgccgcgtat actttttcca gatactcatc gctgatgcct tcaagccaga aaagccgctt
      601 cccatattcc ctgtgtttcc ttatcctgtc gcagaggtcg tcagtaagcc atcccttttt
      661 gcctacaata acaaggttgg tacttatccc ttcattccag aggcattcaa aagcctcaag
      721 gacaagatcc tgtcttttgc gcggttcaat agtcccgacc attataaaac tcttgttgga
      781 agatatttgt gacaaaatat gatctgcacc ctcgggaatt ccaagagtag gaatggaatt
      841 atcaatgtct gcaccaaggt ggaaccattt aatctcaaac ttatccatcc tgtcaggtac
      901 attagtgctc atccaattag tcagttcgtc tgctacagct tttgaaatgc aaacagcacc
      961 atcaaacaac attattgtat ttatccatga agttacgatt tcctttcctt ctggataaaa
     1021 aaactcagca tgtagtattg gcaaaaggtc atacaatatg aacaatactt ttactccaga
     1081 attatggatt tgcattagaa acttttgttg aaggggtacg atattgggtt gcaaatcaag
     1141 tcccaagaaa acgtcacccc tataataatc tattggagag tctaaaagcc atccattagg
     1201 gcaatctatg aattttagag taaaattact agcatattta tatccaggtt cttccaatga
     1261 ggcatagact ggttctactc tgtaaccggt tggaggatta aatatcattt cttttaatat
     1321 gcttcttgtc acacgttgta tacctgtctt tgtgtctctt tgaataagtt cagaaatatc
     1381 gacaaataat tgctttaagc caaccttttt aggttggctt tctacaatcg ctgtcgccag
     1441 tttaagcaat tcctcttgcg attgagaata cccatcaata aatgctattg catttataag
     1501 agaatgtatg ccatattttg aattatcgta aaatgtttct attgtttcaa aatacaattg
     1561 tgcacatttt gaaggagaat gattggcaag tatttcttct cttgctcttg tgcataattg
     1621 ttctctttga ttatcatttt tccacaatgt ctctagggct gttctaaggt cttc
//
LOCUS       DAOSRI010000335         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_67566_length_1668_cov_3.316181, whole genome shotgun sequence.
ACCESSION   DAOSRI010000335 DAOSRI010000000
VERSION     DAOSRI010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67566_length_1668_cov_3.316181"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK717_08110"
     CDS             complement(<1..297)
                     /locus_tag="PK717_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47657.1"
                     /translation="MGLSDLPIQPFFTKRFKNHIASLRVSASYIFVGTESRGASILAR
                     AFAKTLNCEKQDSDFCGVCHSCRMIEAQTHPDVKIYDPGDARFGIGLARQVQQES"
     gene            complement(297..626)
                     /locus_tag="PK717_08115"
     CDS             complement(297..626)
                     /locus_tag="PK717_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOR47658.1"
                     /translation="MKLIAAIIQQEDANEATAELLQNGYKITKLRGIGGFLEEGNLVL
                     LIGVEDAHVPKVLQIFQKTCVARDRYINALPFVPMEGMPPLSFPVKVTVGGAKIFVLP
                     VEEFHML"
     gene            complement(623..1225)
                     /gene="tmk"
                     /locus_tag="PK717_08120"
     CDS             complement(623..1225)
                     /gene="tmk"
                     /locus_tag="PK717_08120"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOR47659.1"
                     /translation="MSGKFIVFEGVDGAGKTTQMKLLHKKLTTIGHDCIMTYEPGDNS
                     VGVSVRKFLIEKDLDAVSEALLFAFDRAVHIREVVRPAIDEGKIVLCDRFLMSSLVYQ
                     GIARGLGEEWINEINSKGLGGLVPDVTLVFDIAVDMALKRVQNANRFENTSLQEKVRE
                     GFIKKSGETPNTFLIEAGRSEEEVFLSVWEKVSWLLKEEK"
     gene            complement(1222..>1668)
                     /locus_tag="PK717_08125"
     CDS             complement(1222..>1668)
                     /locus_tag="PK717_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47660.1"
                     /translation="SGKDTQKPPTKKPSTEKPGNGGKDTTPGTKPPIDKPNPEPQPQP
                     EPEPQPQPEPEPQPEPEPRYFFVSPSQASASVGEFVRFDYVFNYEGVSWVTMYVDGVQ
                     VDTATSFPWSIVWAPRSAKTYNLLFILYNDAGGRVAEYSRSYTVYP"
BASE COUNT          402 a          432 c          366 g          468 t
ORIGIN      
        1 cgattcctgc tgcacttgtc ttgcaagacc tattccaaat cttgcatcgc cgggatcata
       61 gatcttcaca tcaggatgcg tctgggcttc aatcatcctg catgaatggc aaacgccaca
      121 aaaatcggaa tcctgcttct cacagtttag cgtttttgca aaggccctcg ccaaaatgga
      181 ggcacccctt gactctgtcc caacaaatat atagcttgca gaaaccctta gcgaagctat
      241 gtggttttta aaacgcttgg taaaaaacgg ttgtatgggt aaatccgaaa gacccatcac
      301 agcatgtgga attcttcgac aggaagcacg aaaatctttg cgccaccaac ggtcactttc
      361 accgggaacg aaagtggtgg cattccctcc atcgggacaa acggcagggc gttgatgtat
      421 ctgtctcttg caacacatgt tttctgaaaa atctggagaa cctttggtac atgggcgtct
      481 tcgacaccaa tcaaaagaac aaggttgccc tcttcaagaa aaccgccaat acctcttagc
      541 tttgttattt tatagccgtt ctggagaagt tctgcagttg cttcgtttgc gtcttcctgc
      601 tggattattg cggcaataag tttcattttt cctccttgag caaccacgaa accttttccc
      661 acactgagag gaacacctct tcttcgctcc tgccagcttc aattaggaaa gtgtttgggg
      721 tctctcctga tttttttata aatccttccc tcaccttttc ctgcagggaa gtgttctcaa
      781 acctgttagc attttgaact cttttgagag ccatgtcaac tgcgatatca aataccaaag
      841 ttacgtctgg gacaagccct cccagcccct tgctgtttat ttcgtttatc cattcttcac
      901 caagccccct ggcaatcccc tggtaaacca gcgaggacat aagaaacctg tcacaaagca
      961 ctatcttgcc ttcatcgatg gcgggacgca caacctccct tatatgaaca gccctgtcaa
     1021 aagcaaaaag gagtgcctcc gaaactgcat caaggtcctt ttctatcaga aactttctga
     1081 cactcacacc aaccgaattg tcaccaggct cgtaagtcat gatgcaatca tgccctatag
     1141 ttgtgagttt tttgtgaagg agtttcatct gtgtggtttt tcctgcgccg tcaacaccct
     1201 caaaaacaat gaatttacca ctcatgggta caccgtgtat gaccttgaat actcggccac
     1261 ccttccaccc gcatcattgt agagaataaa aaggagatta taggttttgg ccgatctcgg
     1321 cgcccagaca attgaccatg ggaagctcgt tgctgtgtcc acctggacac cgtccacata
     1381 catcgttacc cagctgaccc cttcgtaatt gaaaacgtag tcaaacctga caaattcacc
     1441 aactgaggct gaggcctggc ttggggagac aaaaaagtac cttggctccg gttcaggttg
     1501 gggctcaggc tctggctgcg gctgtggctc cggttcaggt tggggttgtg gctctggatt
     1561 tggcttgtca attggtggtt ttgtgccggg agtcgtgtct tttcctccat tgccaggctt
     1621 ctcggtggaa ggctttttgg tgggtggttt ctgggtgtct ttccctga
//
LOCUS       DAOSRI010000336         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_70616_length_1620_cov_3.672204, whole genome shotgun sequence.
ACCESSION   DAOSRI010000336 DAOSRI010000000
VERSION     DAOSRI010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70616_length_1620_cov_3.672204"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..935
                     /locus_tag="PK717_08130"
     CDS             198..935
                     /locus_tag="PK717_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOR47661.1"
                     /translation="MEQLEKFPFNKEIHIKLDSGKLYIQGEDRQDIEASGNFRNMAFK
                     SLQEDEKLILSYSYKVNWFQWIYAWIGYSLKKEWPTGNLLLKVPQKCNLKANVYSGSL
                     SVEGTDGSANLQVGSGKAIVSQCIGKLKMLLSGAECIVKDFCGSVSIYSYTGDIDFLG
                     VLSTDSPNKIESLTGTIKATLTDPDLKFNAAASTGQITLESEDGTKQDARTRAYGTLG
                     NGTGFLQLVTKIGNIAIKTISTKKHQL"
     gene            complement(926..1351)
                     /locus_tag="PK717_08135"
     CDS             complement(926..1351)
                     /locus_tag="PK717_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47662.1"
                     /translation="MEENVQKQTSKQAPEENEVLYSVIAHISLGIVGIAVLSGMIASV
                     KDSKFAHDHSVFSVINGVLTLVVMFSWYYLFLGLGALMGQQAVYEWYTMGMMLFVIAS
                     FGGALYSAIIASLGKIPFVPWVYPIANRFFKIDTQKPQS"
BASE COUNT          526 a          352 c          316 g          426 t
ORIGIN      
        1 ttattgatgg caaaccggcc ttcaccccat ccaacctggt cattggtaaa ttgaaaaaac
       61 ccgatgagga attgctcaaa ctggcctacc agcttcacga ttgatcgtaa tgcttagagc
      121 acaacaccgc caatagttga accccaattt caactataaa accttgtgaa cattcttcta
      181 atggttagcc ttaaactatg gaacaactgg aaaaattccc cttcaacaaa gaaatacaca
      241 taaagcttga ctccgggaaa ctttacatcc agggtgagga cagacaggac atagaggcat
      301 ctggaaattt tagaaacatg gctttcaaat ccttacaaga agatgaaaaa ctcatattgt
      361 cctactcata caaggtaaac tggttccaat ggatatatgc atggataggc tattctctaa
      421 aaaaagagtg gccgactggc aaccttcttc taaaagttcc acaaaaatgc aatctaaaag
      481 caaatgtata ttcaggttcc ctatctgtgg aaggcaccga tggttctgcc aatctgcagg
      541 tgggatctgg caaggcgatc gtttcccagt gcataggaaa actaaaaatg cttctttcgg
      601 gcgctgagtg cattgtaaaa gatttttgcg gttccgtgtc catatacagc tataccggag
      661 atattgattt tttaggggtt ttgtccacag actcgccaaa caaaatagaa tcactcaccg
      721 gaacgataaa agcaacgctc acagacccgg atcttaaatt caatgcagca gcctcgaccg
      781 gacaaataac cctggaatca gaggatggta caaaacagga tgcaagaacc agggcctatg
      841 ggacccttgg caatggaaca ggcttccttc aattggtgac taagattggc aatatagcta
      901 tcaaaactat ttcaaccaag aaacatcagc tctgaggttt ctgtgtatca atcttgaaaa
      961 atctgttggc aatcggatag acccatggca caaaaggtat cttgccaagg gatgcgatga
     1021 ttgctgaata aagggctcct ccaaacgatg ctatcacgaa taacatcatg cccattgtgt
     1081 accattcgta gactgcttgc tgacccatga gcgcacctag acccaaaaaa agatagtacc
     1141 aggaaaacat cacaacaagg gtcaaaacac catttataac cgaaaacaca gagtgatcgt
     1201 gggcaaattt gctgtctttt acagaagcaa tcatgccgga tagcactgca ataccaacaa
     1261 taccaaggga tatgtgggcg atcacactat aaagcacttc attttcttct ggtgcctgtt
     1321 tggacgtctg tttctggaca ttctcttcca tgtagaccat cttagaagaa ttggacagaa
     1381 ggtcaacttc gctcccttta gaatgggtat ttaatctttg gtttactttg aagtttttac
     1441 cggttttttt gtcaaccatc caaaaaccaa tgtgaaattg tacacatcag gttgaatatt
     1501 aagtatcaca aattcacaat gaattttcat gacttattga cttagttccc aaggaaccta
     1561 gactatcaag tgaaagatgt ttccaatcaa tcttatttat ctttatcaat ccgaaagggg
//
LOCUS       DAOSRI010000337         1614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_71018_length_1614_cov_4.387428, whole genome shotgun sequence.
ACCESSION   DAOSRI010000337 DAOSRI010000000
VERSION     DAOSRI010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71018_length_1614_cov_4.387428"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK717_08140"
     CDS             complement(<1..618)
                     /locus_tag="PK717_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR47663.1"
                     /translation="MEAIVKAKNLTKSFGGTVVLENLNFEIAEGESFGFLGPSKSGKT
                     TLIEILAGLTRPTSGEIWVGGVNMGQYPNKARKIVSLVPQTLALYQDFSAHENMRFFG
                     NFINIKNKKLKEISLEALDLFELTHEAHIPIEQLPYSYAKRLSIAVAMLSDPRIILLE
                     SPTEGLDDRSARIITDGLNLLSNRGKTIIMATSYTREIATVCRKVG"
     gene            complement(627..1394)
                     /locus_tag="PK717_08145"
     CDS             complement(627..1394)
                     /locus_tag="PK717_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR47664.1"
                     /translation="MAISPGQLANWLNEALAISQATSDSSANGLQVDASEPVIKLGFA
                     VDACMDAFVEAKNQGCQMLLCHHGLYWKGDDQRAVGLMGNRISYLIKNGISLWACHLP
                     LDMHPEYGNNAGLATILQIKNRKPFGFYHDLAIGFYGEVEGSPTCSELVQRLNKDIPE
                     CKPWVWSFGKKSCKNIGVVSGGGSYAIEEAAKLKLDCLVTGEVFHQDYHTAKELGMNV
                     IVAGHWATETVGILSVGEAVATFFGLESVFINKPTGL"
BASE COUNT          412 a          365 c          333 g          504 t
ORIGIN      
        1 tccaactttt ctacacactg ttgctatctc tctggtgtag gatgttgcca tgattattgt
       61 tttccctctg ttggagagga ggtttaaccc gtcggttatt atccttgctg atctgtcatc
      121 gagaccctct gttggggact ccaggaggat tattcttggg tccgagagca ttgcaaccgc
      181 aatggagagc ctctttgcgt atgagtaagg taattgctct atcgggatgt gggcctcatg
      241 tgtaagctca aataaatcca gggcttcaag agaaatttct ttcagttttt tgttttttat
      301 atttataaaa ttcccaaaaa acctcatgtt ctcatgtgct gagaaatcct ggtagagtgc
      361 gagtgtctgt ggtaccaaag aaactatctt tctggccttg tttggatatt gccccatatt
      421 gaccccacca acccatattt caccagaggt tggtcttgtg aggccagcaa gtatctcaat
      481 cagtgtcgtc ttgccagatt ttgaaggtcc cagaaaaccg aaactttcgc cttctgcgat
      541 ttcaaagttc aggttctcca gaacgactgt tcctccaaaa gactttgtga ggttttttgc
      601 tttaacaata gcttccatcg tgccccctat agacctgtgg gtttgttaat gaaaactgat
      661 tcaagcccaa agaatgtagc tacagcttcg ccaactgaaa ggatgcctac ggtttcagtt
      721 gcccagtggc cagcaacaat aacgttcatc ccaagctctt ttgccgtgtg ataatcctgg
      781 tggaacacct ctccagtcac aagacaatca agtttgagct tggcagcttc ctcaatggcg
      841 tatgaaccgc cacccgaaac caccccgata tttttacaac tttttttacc aaaactccag
      901 acccagggtt tgcattcagg aatgtccttg tttaacctct gcaccaattc actgcatgtg
      961 gggctacctt caacttctcc gtagaaacca attgcaaggt catggtaaaa accaaaaggt
     1021 tttcgatttt ttatttgcag gattgtagca agaccagcat tatttccgta ttccgggtgc
     1081 atatctaatg gaagatggca tgcccagagg gaaataccat ttttaatcag gtaagaaatt
     1141 ctgtttccca tgagacccac tgccctctga tcatcaccct tccagtacaa tccatggtgg
     1201 cagagcaaca tctgacagcc ctgatttttt gcctcgacga aagcatccat acatgcatct
     1261 acagcaaaac cgagctttat cactggctct gatgcgtcaa cctgcaatcc attggccgac
     1321 gaatcgctgg ttgcttgtga gatcgcaaga gcttcattga gccagtttgc taattggcct
     1381 ggtgaaatag ccattagcaa acttcttcct gttctatgta ttgcattact ggcttatttt
     1441 atttggttac attcgctttg tcaaatacag gttttatttg gcttgactct ctttttgcga
     1501 aaggtataaa gatagtaagg agtaaaaata gtgaaaaaga gacttctggc gactattttg
     1561 ataatgtcta tcatttttgt gggaaatacc tttgctgatg attgtgttat tagc
//
LOCUS       DAOSRI010000338         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_71128_length_1613_cov_2.730424, whole genome shotgun sequence.
ACCESSION   DAOSRI010000338 DAOSRI010000000
VERSION     DAOSRI010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71128_length_1613_cov_2.730424"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK717_08150"
     CDS             <1..838
                     /locus_tag="PK717_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47665.1"
                     /translation="GKTFEERIAKFIPADFNVAEKRNMAGENVFVLAKSPKSGIAQYM
                     AMIVDDSGGELKTVKQAKPFRAENVQLAPERQWGAKLSGDEKGYAIMIKLGGDRLEDT
                     FVKVLDNSFNEIPNELGPGYVTRDFEDVDFDGNFDIIVLDTRWSRLAKILGSQPYTQR
                     VCTYSDGKFVLDDPRFKDRYDAGIKAFKAKLDEEAQYENEAFYYSLNLLLVQEALGQT
                     KDGLSKFQAFIGTLKDEKLIGLGKELLGAFTSQASKGEKLSDPLWKPYSAQAWEPLFW
                     KD"
     gene            900..975
                     /locus_tag="PK717_08155"
     tRNA            900..975
                     /locus_tag="PK717_08155"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:933..935,aa:Gln,seq:ctg)
     gene            990..1064
                     /locus_tag="PK717_08160"
     tRNA            990..1064
                     /locus_tag="PK717_08160"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1023..1025,aa:Glu,seq:ctc)
     gene            1159..>1613
                     /locus_tag="PK717_08165"
     CDS             1159..>1613
                     /locus_tag="PK717_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47666.1"
                     /translation="MIRTKFLGICLSCIILVGFSFPINPQILSVVSVPTRLHFELDAY
                     CSIGKLDHPAFANEEFPQFSSFPRFKVAIVPVATDTRLNGTNLKRLINVKDFADYISF
                     HAPNLKLVQLGESYTYYQNGQPVNTGKLYNALTVSSDDPKSVKERIEATL"
BASE COUNT          453 a          359 c          383 g          418 t
ORIGIN      
        1 tggcaaaacc tttgaggaac gcattgcaaa gtttatcccg gctgatttca atgttgcaga
       61 aaaaagaaac atggctggtg agaacgtctt tgtcctggca aaaagtccca aatcgggcat
      121 cgcccagtac atggcaatga ttgtggatga ttctggcggg gaattgaaaa cggtaaagca
      181 agcaaaacct ttcagggccg aaaatgtcca gctggcacca gaacgccagt ggggagcgaa
      241 gctttctggt gatgaaaaag gttatgcaat catgataaag cttggtggag acagacttga
      301 agatacattt gtcaaagtcc ttgacaattc cttcaatgaa atcccgaacg agcttggccc
      361 tggctatgtc acaagagatt ttgaggacgt agatttcgat ggcaatttcg acatcatagt
      421 gcttgacacg cgctggtcca ggctggcaaa aatcttgggg agccagcctt atacgcaaag
      481 agtctgcacc tacagcgatg gcaagtttgt gcttgacgat ccaaggttca aggaccgcta
      541 tgatgctgga ataaaggcgt ttaaggccaa gcttgatgaa gaagcgcaat atgaaaacga
      601 ggctttctac tattccctga accttctgct tgtccaggag gcgcttggac agacaaaaga
      661 tggactctcg aagttccagg cctttatagg gacactgaaa gacgagaaac ttatcgggct
      721 cggaaaagaa ctccttggtg cttttacctc ccaggcttca aagggagaaa agctgtccga
      781 tcctctctgg aaaccctaca gtgcccaggc ctgggagcca cttttctgga aggattaaac
      841 ttggctcagc aaatattact tgacctaaaa accgacttgt attatataaa catacgcgct
      901 gccggatggt gtaattggta acacgcctga ctctggatca ggagactcta ggttcgagcc
      961 ctagtctggc agccaaaaaa gaggagaacg gcgccatagt ataatggtca gtacaagggg
     1021 ctctcaatcc ctagatagga gttcgattct ccttggcgct accaattgcg tcaaggaaac
     1081 accataaagg cccacaaagg gccttttttt gttttttgtg gtcttgaggg aattgccact
     1141 caatggtatt atttgggcat gatcaggaca aagtttttgg ggatatgcct ttcctgcatt
     1201 attcttgtgg ggttttcgtt tcccataaac cctcaaatcc ttagcgttgt ctctgttcca
     1261 acaagacttc attttgaact tgatgcctat tgctcgatag gcaaacttga tcatcctgct
     1321 tttgcaaacg aggagtttcc tcaattttct tcctttccaa gattcaaagt ggccattgtg
     1381 cctgttgcta ccgatactag actcaatgga acaaacctca aaagactcat taatgtaaaa
     1441 gattttgcag attatatttc ttttcatgcc ccaaatctca agcttgtgca gctcggtgaa
     1501 agctacacct attatcaaaa tggccagccg gtcaacacgg gcaagcttta caatgcgctc
     1561 acggtgtcgt ccgatgatcc aaaaagcgtc aaggagagga ttgaagcgac cct
//
LOCUS       DAOSRI010000339         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_71768_length_1603_cov_3.079457, whole genome shotgun sequence.
ACCESSION   DAOSRI010000339 DAOSRI010000000
VERSION     DAOSRI010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71768_length_1603_cov_3.079457"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..901)
                     /gene="accD"
                     /locus_tag="PK717_08170"
     CDS             complement(98..901)
                     /gene="accD"
                     /locus_tag="PK717_08170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOR47667.1"
                     /translation="MPEDNVQKCKGCNRPLDMPAFEANMRICPKCGSHEQVPVRERIK
                     MTVDEGSFKELWGHLVSKDLIGFVDSKPYADRIIETINKTGENDAIVTGDASIHETKV
                     AIGFMNFGFIGGSMGVVVGEKIMRLAEHALKEKVPMIIFSQSGGARMQESVFSLMQLA
                     RMSGAIYRFQEHGGLYISVICNPCTGGASASFAFQGDIIISEPGALIGFAGPRVIEQT
                     IKQKLPPGFQTAEFLQEKGFLDKVVDRKNMRWVLGKILSLYRGIPMEVA"
     gene            complement(894..>1603)
                     /locus_tag="PK717_08175"
     CDS             complement(894..>1603)
                     /locus_tag="PK717_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOR47668.1"
                     /translation="KRDDADLMFAGGVEASLIPLGVIGFANMKALSTRNDDPEKASRP
                     FDKDRDGFVMGEGGAVLLLEEYEHAKARGAQIYCEIKGAGYSSDAYHITQPDPDGWGA
                     KACMKNAMRKAGVGIENIDYINAHGTSTPLNDRIEAKAIADVFGDRTGEILVNSTKSM
                     VGHLLGAAGAVEAAAVALQIVNKTVHPSINFENPDPESKLNIAAKAVKKEVKYALSNS
                     FGFGGHNCCIVFGRVDA"
BASE COUNT          403 a          419 c          330 g          451 t
ORIGIN      
        1 tttgtaaggt caagggaacg tgcaattgcc tgcttgcgaa actcttcaag tttttgttcc
       61 agttcaacta ttggtttttc aaaatcaagg taaaccatca ggccacctcc atcggaatac
      121 cacgatagag tgaaagtatc ttgccaagca cccagcgcat gttcttcctg tcaacaactt
      181 tgtccaggaa gcctttttct tgtaaaaact cagctgtctg gaaacctggc ggtagtttct
      241 gttttatcgt ctgctcgata acccttgggc ctgcaaaacc tatcagtgcg cctggttcgg
      301 atatgattat gtcaccctgg aaagcaaaag aagctgatgc accgccagta caaggattac
      361 atatcaccga aatatacagg ccgccatgct cctggaacct gtaaatggca ccagacattc
      421 tagcaagctg catgagggag aaaacagact cttgcatcct ggccccgcct gactgggaga
      481 aaatgatcat tggcaccttt tccttgagag cgtgttcggc aagcctcatt attttctcac
      541 ccacaacaac gcccatggac ccaccgataa acccgaaatt cataaaacca atggcaacct
      601 ttgtttcatg gattgatgca tcgccagtca ctatggcatc attctcgcct gttttattga
      661 ttgtttcaat aattctgtcg gcatagggtt ttgaatctac aaaaccaatg aggtcttttg
      721 aaaccagatg tccccaaagc tccttgaagg aaccttcgtc tacggtcatc ttgattcttt
      781 ctctcacagg cacctgttca tggcttccac acttcggaca aatcctcata tttgcctcaa
      841 aagcaggcat atctagaggt ctgttgcaac ctttgcattt ctggacatta tcttcaggca
      901 tcaaccctcc caaaaacaat acagcagttg tgtccaccaa acccaaacga atttgaaagt
      961 gcgtatttca cttctttttt aacagctttt gcggcaatgt tgagtttgct ttcgggatca
     1021 gggttttcaa aatttattga agggtggacg gttttattga ctatctgaag tgccactgcg
     1081 gcggcctcta ctgcaccagc ggcgccaagc aggtgaccca ccatcgactt tgtcgagttg
     1141 acaagtattt ctccggttct gtctccaaat acatctgcaa tggccttggc ctctatcctg
     1201 tcattgagag gtgttgaggt gccatgggca ttgatataat ctatattctc aatacccact
     1261 ccggccttgc gcattgcgtt cttcatgcaa gcttttgcgc cccagccatc cgggtcaggc
     1321 tgtgtaatgt gataagcatc gcttgaataa ccagcgccct ttatttcaca ataaatctgt
     1381 gcacccctgg cttttgcatg ctcatactct tcaagcagca atacagctcc accctcgccc
     1441 ataacaaagc catcgcggtc cttatcaaat ggacgtgatg ctttttccgg gtcgtcattt
     1501 cttgtggaaa gagccttcat gtttgcaaag ccaatgacac ccagcgggat gagagaagcc
     1561 tctacgccac ctgcgaacat gagatctgcg tcatcgcgct tga
//
LOCUS       DAOSRI010000340         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_71781_length_1603_cov_2.569767, whole genome shotgun sequence.
ACCESSION   DAOSRI010000340 DAOSRI010000000
VERSION     DAOSRI010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71781_length_1603_cov_2.569767"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..433)
                     /locus_tag="PK717_08180"
     CDS             complement(41..433)
                     /locus_tag="PK717_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOR47669.1"
                     /translation="MARPKKWRKVCSLPDNSRFGPLDSPADASHVNMTVDEYEAIRLI
                     DLEGFTQEECAQQMNIARSTVQGIYGEARKKLAESLVIGKILHIEGGEYQLCDGSRME
                     CGRGCHRRRRGRHRNQNGGFYENSHSRE"
     gene            complement(496..762)
                     /locus_tag="PK717_08185"
     CDS             complement(496..762)
                     /locus_tag="PK717_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOR47670.1"
                     /translation="MPGKDGTGPTGTGPMTGRALGLCTDTEESKRGFFRGRGLGLGLG
                     RGRGFGRRFATRQASPENQKTWLQEQKVVLQDRLKTIDKQLEDL"
     gene            1005..>1603
                     /locus_tag="PK717_08190"
     CDS             1005..>1603
                     /locus_tag="PK717_08190"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOR47671.1"
                     /translation="MRKYEQKQDWMKTPVELPELPWAKDALAPIISEETINFHHGKHH
                     NAYVVKTKELIAGTDLEDKSLEEIMIFAWDKPEHKVLYNNAAQVWNHNFYWKCLTPKD
                     KSVMPDELRDVLIENFGSVEAFKKELVAAGVGQFGSGWAWVMLEDGKLSISKTPNAED
                     PWTEKRSPVLTLDVWEHAYYLDFQNRRQAYLEAVIDNIV"
BASE COUNT          419 a          379 c          353 g          452 t
ORIGIN      
        1 gcgtccgaag tgtgcgctaa cgcttgagtt caaggttttt tcattcacgg gaatggctat
       61 tttcataaaa tcctccattt tgatttctat gtctgccacg tctgcgtctg tggcaacccc
      121 gcccgcattc cattctggaa ccgtcacata attggtattc cccgccttca atgtgcagga
      181 ttttgccaat caccagggat tcagccagtt tttttcgggc ttcgccataa atcccctgga
      241 cggtgctgcg agcgatattc atttgctggg cacactcctc ctgagtgaaa ccctctaaat
      301 cgataagtct gatggcttcg tattcgtcaa ctgtcatgtt gacatgactt gcatcagcgg
      361 gtgaatcaag tgggccaaac ctgctgttat caggtagtga gcatactttt ctccattttt
      421 ttggccttgc catggttttt cacctccgtt aaaaagtaga gatagctgga gatagctcca
      481 gttatctcgg atagtttata gatcttccag ttgtttgtca atcgtcttga gacggtcttg
      541 caagactact ttttgctctt gaagccatgt tttttggttt tccggagaag cttgccttgt
      601 agcaaagcgt ctgccgaaac cacgtccgcg gccgagacca agacccagtc ccctgccacg
      661 aaagaatcca cgtttgcttt cttctgtgtc tgtgcaaaga cccaaagctc ttccggtcat
      721 cggtccggtg ccagttgggc cagttccatc ttttccaggc atgattaaaa cctcctttat
      781 agtttatggc atatgccagt tacttctatt gtaccttgtt tctggcatat gtcaataact
      841 gtattcgaca agtttcaaaa aaagacggtt tttagtacaa agcttgtaca atttgtcaaa
      901 aataatgcct ttggtaaaag ctttggtttt attttgctta tgttttatcc tataatcaga
      961 gacaagaaaa caataaaaaa acgcaaagaa aggacaaacc gaagatgcga aaatacgaac
     1021 aaaaacaaga ctggatgaag acccccgtgg aacttccaga actcccttgg gcaaaagacg
     1081 cccttgcacc cattatttct gaagaaacaa tcaattttca tcacggcaaa caccacaatg
     1141 cctacgtggt gaagaccaag gaactgattg ccggcacaga tttggaagat aaatcccttg
     1201 aagaaatcat gatttttgcc tgggacaaac ccgagcacaa ggtgctttac aataacgccg
     1261 cccaggtgtg gaaccacaat ttctactgga agtgtttgac ccccaaagac aaatcggtaa
     1321 tgcctgatga actgcgcgat gttctcatcg agaatttcgg ctcggttgaa gctttcaaga
     1381 aagaactggt tgctgctggt gtgggacagt ttggcagtgg ctgggcttgg gtgatgcttg
     1441 aggatggcaa gctttcaatt tcaaagaccc cgaacgcgga agatccctgg actgagaaac
     1501 gctcacccgt gctcaccctg gacgtctggg agcatgctta ttatctcgac ttccaaaatc
     1561 gccgccaggc atatctggaa gcagtcattg acaatattgt caa
//
LOCUS       DAOSRI010000341         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_71822_length_1602_cov_4.248222, whole genome shotgun sequence.
ACCESSION   DAOSRI010000341 DAOSRI010000000
VERSION     DAOSRI010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71822_length_1602_cov_4.248222"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK717_08195"
     CDS             complement(<1..439)
                     /locus_tag="PK717_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR47672.1"
                     /translation="MSIPKKFSIGNLNIKVPIIQGGMGVGISLSGLASAVANQGGVGV
                     IATAGIGMFEPDFNTNFLEANMRALRREIRRAREMTNGVLGINVMVALSNFADVVKTA
                     IQEGIDIIFSGAGLPLNLPSYLMGSLKTKLVPIVSSARAANLIS"
     gene            724..1416
                     /locus_tag="PK717_08200"
     CDS             724..1416
                     /locus_tag="PK717_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOR47673.1"
                     /translation="MAIKNSISVENALEILSKEGKQENKEGMAKFGINTEKAFGVSVV
                     KIREVAKMVGKSHEKAQKLWETGFHEARIMACLIDNPKMVIREQAESWALEFNSWDLC
                     DQCCMNLFDKLPFSYELAAEWAEREEEYVKRAGFAIMASLALHDKKAPDKKFLPFLNS
                     IERQSTDPRNFVKKAINWALRQIGKRRPGMMDKVETLAGKLAESDDKNARWVGKDALR
                     EFEKKNLSAKEG"
     gene            1509..>1602
                     /locus_tag="PK717_08205"
     CDS             1509..>1602
                     /locus_tag="PK717_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOR47674.1"
                     /translation="MQTIILLVISNIFMTFAWYGHLKFKEAPLWQ"
BASE COUNT          518 a          342 c          344 g          398 t
ORIGIN      
        1 ttgagatgag gtttgctgcc cttgctgatg aaacgattgg caccagcttc gtcttcagtg
       61 atcccataag atacgacggg aggtttaatg ggagcccagc accagaaaag attatgtcaa
      121 tcccctcttg aatggcggtc ttcactacat cagcaaaatt cgagagagca accatgacat
      181 tgatgcccag cacgccattt gtcatctctc tggcccgcct gatctcgcgc cttagcgctc
      241 tcatgttggc ctcgagaaaa tttgtgttga aatctggctc aaacatacct attccagcag
      301 tagcaataac acctactcca ccctggtttg caacagcact tgcaagaccg gaaagggaga
      361 tacctacacc cattccaccc tggattatgg gcaccttgat gttcagattg cctattgaaa
      421 acttctttgg aatactcatt ttctgcgctc cttttttatt tattgtcctt tgtcaaatga
      481 gcacagtcct taccgttatc aatcaacaaa aaacgtttct actcttcatt atcatagtgt
      541 gaaccaattt aacaaggatt attccgggtg gatcagttaa tagtagcgac tacttataga
      601 tagttttaac tatacaaaaa atcaggatca ttgggaatca ttcaaataag tttggatatt
      661 tttgaaatgg tttgtagaat ttataaaaaa caaaagactt ctcaggctca aaaggagaca
      721 caaatggcca taaagaattc catctcggtt gaaaatgccc tggaaatcct ttcaaaagag
      781 ggcaaacagg aaaacaaaga ggggatggcc aaatttggaa taaataccga aaaagcgttt
      841 ggcgtctctg ttgtgaaaat cagagaagtt gcaaaaatgg tcggcaagag ccacgaaaaa
      901 gcccaaaaac tctgggaaac tggcttccat gaggctagaa tcatggcatg ccttatcgat
      961 aatccaaaaa tggtaataag ggaacaagcc gagagttggg cacttgagtt caattcatgg
     1021 gacctttgtg accagtgctg catgaatctt tttgacaaac tgcctttctc atacgagctt
     1081 gcagcagaat gggcggaaag ggaggaagag tatgtaaaaa gagctggttt cgcaatcatg
     1141 gcatccctgg cactgcacga caaaaaagca cccgataaaa aattcctccc atttctgaat
     1201 tcaatcgaga gacagtcgac agatccaaga aatttcgtaa aaaaggccat aaactgggct
     1261 ctcaggcaaa tcggcaaaag aaggcccggc atgatggaca aagttgaaac tctggctgga
     1321 aaacttgcag aaagcgacga caagaatgcc agatgggttg gcaaggacgc cctcagggag
     1381 tttgagaaga aaaatctgtc ggcaaaagaa ggttaatcag tcaccaacac aaacattttt
     1441 ctattttggt gccttgaaaa ataatcgcgt gcgacaatca ttttttaggg aaaaccaaag
     1501 gagaaaaaat gcaaaccata attctgctcg tcatttcaaa catattcatg actttcgcat
     1561 ggtacggaca cctcaaattc aaggaagccc cgctctggca gg
//
LOCUS       DAOSRI010000342         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_72348_length_1594_cov_2.926576, whole genome shotgun sequence.
ACCESSION   DAOSRI010000342 DAOSRI010000000
VERSION     DAOSRI010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72348_length_1594_cov_2.926576"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK717_08210"
     CDS             <1..457
                     /locus_tag="PK717_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR47675.1"
                     /translation="EKGAAATLVMIFAVFNGIGRPIFGALTDKITPKWSAILSFVLIF
                     VASALMLAAGQGSVGLYAVAFCIFWLCLGGWLAIGPAATAGFFGLEGYAQKYGVVFSS
                     YGLGAIIGGIISGSAKDAFGSYTIAFYPTAGMAILGIIIALILLKPPKK"
     gene            632..>1594
                     /locus_tag="PK717_08215"
     CDS             632..>1594
                     /locus_tag="PK717_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR47676.1"
                     /translation="MNEFFFPNSIAIFGISSSPGNLGIGILENLERFSFKGDVYLIGE
                     KGGSVRGKTILKGIAEVPGIPELAIFIIPAKGIPGKLEECGKKGIRHVIIESGGFSEF
                     GKEKEALEKEVLAIAKQWGIRILGPNCLGTVNLENGLVLPFVRFHETFLQKGGISLIS
                     QSGGLLHDILLLLSCDNLGINKLVSAGNKLMLDECDFLEYFASDPATKTIGLYLEDIK
                     DGRRLFRLATSTNKPVILLKANESSGSSQIAQFHTASLAGDDLIADNILCQAGIHRVR
                     TLDEMISTIKIFSLPLMRGQRLAVIARSGGHAVMAADAVYRHGFR"
BASE COUNT          418 a          364 c          374 g          438 t
ORIGIN      
        1 tgaaaaaggt gcagccgcaa cactggttat gatatttgct gttttcaacg gtatcggccg
       61 cccaatcttt ggtgccctta cagataaaat aacccccaag tggtctgcaa tcctgtcatt
      121 cgttctcatt tttgtcgcct cagccttgat gcttgcagcc ggtcagggat ccgttggtct
      181 gtatgcagtc gccttttgca tattctggct ctgtctcgga ggctggcttg caataggccc
      241 ggcagcaaca gcaggtttct ttggtcttga gggatatgca cagaaatatg gtgttgtgtt
      301 tagctcatac ggtctcggtg caatcatcgg gggcatcatt tccggaagcg ccaaggacgc
      361 ctttggaagc tacacaatag cgttctatcc tacggcgggc atggcaatcc ttgggatcat
      421 tattgctctt attctcctga aaccgccaaa aaaatagaaa ccataatcga taaagaaggt
      481 aaaaaggggc agacttcaat ggggtttgcc tttttttatt gcacaaaaga ttcctaagca
      541 aatttcttat aatctatcca aaatttttgt gatacaatcg ccttacctgc ataagttgca
      601 tagaacattt aaaattgaag gggaagcatt tatgaacgag tttttctttc caaacagtat
      661 tgccatattt ggcatatctt cttcgcctgg taatttaggg attggaatat tggaaaatct
      721 ggagcgtttt tctttcaaag gggatgttta tctgattgga gagaaaggcg ggtcggtgag
      781 gggaaaaact atcttgaaag gcattgcaga ggttcccggc attccagaac ttgctatttt
      841 tataataccc gccaaaggca tccctggcaa acttgaagaa tgcggcaaaa aaggcatccg
      901 gcatgtaatc attgagtcag ggggtttctc tgagtttgga aaggaaaaag aagcattgga
      961 gaaggaggtt ctcgccatag caaaacaatg gggcattaga atcctaggac caaactgcct
     1021 cggcaccgtg aatctggaaa atggcctggt tttaccattt gttcgttttc acgagacatt
     1081 tttacagaag gggggcatct cgcttatctc ccagagcgga ggtctcttgc acgacatact
     1141 cctccttttg tcctgtgaca accttggaat caacaaactc gttagtgctg gcaacaagct
     1201 gatgctggac gagtgcgatt tcctcgagta ctttgcttct gatcccgcaa caaaaaccat
     1261 cggcctctac ctggaagaca ttaaagacgg aaggcgcctt tttcgccttg caacttctac
     1321 caataaacca gtcatcctcc tcaaggccaa tgaaagttcc ggtagttctc aaattgcgca
     1381 atttcacacc gcctcccttg ctggtgatga tcttattgca gacaacatcc tttgccaggc
     1441 aggaatccac agggtccgca cactcgatga aatgatcagt accatcaaaa tcttttccct
     1501 gccactcatg agaggtcaaa ggcttgcagt gatagcacgg tccggaggac acgcggtcat
     1561 ggcagctgat gctgtatacc gccacggttt cagg
//
LOCUS       DAOSRI010000343         1589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_72692_length_1589_cov_3.742503, whole genome shotgun sequence.
ACCESSION   DAOSRI010000343 DAOSRI010000000
VERSION     DAOSRI010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72692_length_1589_cov_3.742503"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1211)
                     /gene="mnmA"
                     /locus_tag="PK717_08220"
     CDS             complement(225..1211)
                     /gene="mnmA"
                     /locus_tag="PK717_08220"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR47677.1"
                     /translation="MAKILVGMSGGTDSTAAVLVLRSYGHEVCGASLPICGRESIDTA
                     IKASEQIGIKHYVIPIQNLFKTQVIEYFKNSIIRNETPNPCVMCNQMVKMHVLHKFAM
                     QMGFEYIATGHYAKKISYAGHITVAKADYEIKDQSYMLARVDEVILQRCLFPLGTLTR
                     AEAESKISGIPTTPPSQDACFINVPMKEWIAREIGIGQPGDIVDTDGNVIGQHCGLNA
                     FTHGQREGIGLADGPWYVVRKDVKNNTLVVGHKENVQVSRFRVIDLRLISDDKPQVMI
                     RYRSNPVECKIEHESNGDIFVSTKTPVFAPTPGQFAVFYSRNILIGSAIITD"
     gene            complement(1211..>1589)
                     /locus_tag="PK717_08225"
     CDS             complement(1211..>1589)
                     /locus_tag="PK717_08225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR47678.1"
                     /translation="GAEEMIESLFRKYKLGLISNTERSSGKHLTMAYHNLMKRFSVTY
                     FSDERKLRKPHPETYLTTAAELAVDPIDCVMVGDDEEKDCTPAVVCGMRAILFADPKR
                     GIKSDFSPQASTLSQLAQILEKM"
BASE COUNT          392 a          343 c          367 g          487 t
ORIGIN      
        1 gatatgaatt tttcaagcat tccctttgtg gtcctgcatc ttgtctgata tggagttttc
       61 acaaagggaa tgcttgaaaa attcatatca gacaagatgc aggaccacct gaggatcgaa
      121 agaacacctc atccggtaga atttgagatg tatgaattga tctgagctaa caagaaaaac
      181 aggataccaa aaggcccttt ccgattggga agggcctttt taatttagtc ggtgattatt
      241 gctgaaccga tcaggatatt tcttgaatag aagactgcga attgacctgg tgttggtgca
      301 aaaacaggtg tttttgtaga cacaaatatg tcgccattgc tttcatgctc gattttgcat
      361 tcaacggggt ttgatctgta gcgtatcatc acttggggtt tatcatcaga aatcagcctt
      421 agatcaatta ccctgaatct tgagacttgc acattttctt tgtgcccgac tacaagcgtg
      481 ttgtttttta cgtcttttct gacaacatac catggaccat ctgcaagacc aatgccctct
      541 ctttgcccat gggtaaaagc gttcagtccg caatgctggc caatgacgtt gccgtcagta
      601 tccacaatat caccgggctg gcctatcccg atttccctgg ctatccattc cttcattggg
      661 acattgatga aacaggcatc ctggctgggg ggtgtggtgg gaatgccgct gattttggat
      721 tctgcctctg cccgcgtgag cgttcccagc gggaagagac acctttgaag tatgacctca
      781 tcaaccctgg caagcatgta gctttggtcc ttgatttcat aatcagcctt tgcaacggta
      841 atgtggcctg cgtaggagat tttttttgcg taatgaccag tggcgatgta ttcaaaaccc
      901 atttgcatgg caaacttatg cagaacatgc atctttacca tctggttgca catgacacac
      961 gggtttgggg tctcgtttcg gattattgaa tttttgaagt attcgataac ttgcgttttg
     1021 aaaaggtttt ggatcgggat cacatagtgt ttgattccaa tctgctcgct ggcttttatg
     1081 gctgtgtcga tgctttctct accacagatc ggaagtgacg cgccacacac ttcatgcccg
     1141 taagacctca aaacaaggac tgcggctgtg gagtctgtac cgccagacat tccgacaagt
     1201 atttttgcca ttacattttt tccaggattt gagccaactg ggagagtgtt gaagcctgtg
     1261 gtgagaaatc agacttgatt cccctttttg ggtctgcaaa cagtattgcc ctcatgccgc
     1321 aaacaacagc aggtgtgcaa tctttttctt cgtcatcgcc aaccatgaca caatcgatgg
     1381 gatcgacagc aagttctgcg gcagtagtaa gataagtttc ggggtgtggt tttctcagtt
     1441 ttctttcatc ggaaaaatat gtcacactaa atcttttcat caaattgtga taggccattg
     1501 tgagatgttt tccggaagag cgttctgtat tggatatgag accaagcttg tattttctga
     1561 agagcgattc gatcatttct tcagcgcct
//
LOCUS       DAOSRI010000344         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_72900_length_1586_cov_3.834749, whole genome shotgun sequence.
ACCESSION   DAOSRI010000344 DAOSRI010000000
VERSION     DAOSRI010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72900_length_1586_cov_3.834749"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>1586
                     /locus_tag="PK717_08230"
     CDS             246..>1586
                     /locus_tag="PK717_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOR47679.1"
                     /translation="MPDFINQLIVNTPWEEPKKHWKFVETTRDFVLAEGRRPAGYVVA
                     SGTNDPNDPGTYVEIGLANHIRNKVNDWRTNDYPGITQITRELLSFWKNQDPDQPNKF
                     FFCQIEAIETLIWLTEVEGYSSPDLMSILCNNESKKHSFIGDGGSFPRLCSKMATGTG
                     KTVVMAMLVAWQTLNKIAYPNDARFSKYFFVVAPNLTVKERLDVLKPSSTSNYYDKFN
                     IVQPIMRDRLNQAKVLVENWHKLSFEDDEKVSRKKGVDKRGAKSDLAFVREVLGDLSR
                     TNGIVVINDEAHHAWRVPSNIKHPDKEAAEEATKWIDGLDRINKVIGIRACFDFSATP
                     FWPSGKKSEEEYLFQWIVSDFGLNDAIESGLVKTPQVVVRDDSIQTKDSKSRLYHIYM
                     DDEVKQDLNRKANENEQLPDLVSKAYNILGNDWRDTKKAWDKKHSETPPVMISVV"
BASE COUNT          530 a          290 c          339 g          427 t
ORIGIN      
        1 ttgaagataa ttgattgcaa tgagcggtaa tataattttg tttagaatgc cttaaatcgt
       61 ggataatcca gaaaccattt tggagaagaa gcaaaagatt gataaaatgt actggttttt
      121 acttcacaat aaaaaaaggc ggtcttatgg atgccgcccc ggtggtaaga accaccaacc
      181 tccgactggt tacggattgg ttatgttatc gtgtgaattt gcagaaatgt caatagaggt
      241 gaagcatgcc tgattttata aaccagctta ttgtaaacac accctgggaa gaacccaaga
      301 aacactggaa atttgttgaa accacaagag attttgtgct tgccgaagga agaaggccgg
      361 ccggttatgt tgttgcctca ggcacaaatg atccaaatga ccctggaact tatgtagaaa
      421 tcggtcttgc caatcacatt agaaacaagg taaacgattg gagaaccaat gattacccag
      481 gaataacaca gattaccagg gaactgctca gtttttggaa aaaccaggac cctgaccagc
      541 caaacaaatt tttcttttgc cagattgaag caatagaaac gctcatctgg ctaactgaag
      601 ttgaaggtta ttcatcgcct gatcttatga gcatcctttg caacaatgaa tcgaaaaagc
      661 acagcttcat cggtgatggt ggttcattcc ccaggctttg ctcaaaaatg gctactggta
      721 caggtaaaac tgttgtgatg gccatgcttg ttgcctggca aactcttaat aagattgctt
      781 atcctaatga tgccaggttt tccaaatatt tttttgtagt agcaccaaac cttactgtca
      841 aagaaaggct tgatgttctt aagccttcca gcacttcaaa ctattatgac aaattcaata
      901 ttgtccaacc cataatgcgg gaccgcctaa atcaagcaaa agttcttgtt gaaaactggc
      961 acaagctgtc atttgaagat gatgaaaagg tttcaaggaa aaagggtgtt gacaagcgag
     1021 gtgccaaatc agatttagca tttgttaggg aggttctggg agatctttca aggactaatg
     1081 gcattgtagt cataaatgat gaagcacatc acgcctggag ggtgccatca aatatcaagc
     1141 atcccgacaa agaagctgca gaagaggcca caaaatggat tgatggcctg gatcgaataa
     1201 acaaagttat tggaatcaga gcctgctttg atttttcggc aacacccttc tggccttcag
     1261 gaaaaaagag tgaagaagag tatctcttcc aatggattgt ttcagatttt ggcctcaatg
     1321 atgctattga atcgggactt gtaaaaacac ctcaagttgt tgttagagac gattcaatac
     1381 agacaaaaga ttccaaatct aggctttacc atatttatat ggatgatgaa gtaaagcagg
     1441 atttaaatag aaaagcaaat gaaaatgagc aattaccaga tttggtttca aaagcttaca
     1501 atatacttgg taatgattgg agagacacta aaaaagcctg ggataaaaaa cattctgaaa
     1561 caccacctgt aatgatctca gttgta
//
LOCUS       DAOSRI010000345         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_73108_length_1583_cov_4.810209, whole genome shotgun sequence.
ACCESSION   DAOSRI010000345 DAOSRI010000000
VERSION     DAOSRI010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73108_length_1583_cov_4.810209"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK717_08235"
     CDS             complement(<1..350)
                     /locus_tag="PK717_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47680.1"
                     /translation="MIKTKNKPQAVIFDFWNTLIYDLPDVEEKRGKYRIEKIHEILIR
                     HDIRVSISDVSFAYEAAGKMITELAKNNRALTIREQIRLMAETIRFIPDRELIEKLED
                     AYNRANLLFLSPLVP"
     gene            444..761
                     /gene="rpsO"
                     /locus_tag="PK717_08240"
     CDS             444..761
                     /gene="rpsO"
                     /locus_tag="PK717_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR47681.1"
                     /translation="MDVLRDDTVATGKEKILMLDKDKKRQIIEKFATHEGDTGSPEVQ
                     VALLTQRINELSAHLLTHKHDFTSRRGLLRMVGERHRMLGYLRSIEENRYLKLISALG
                     LRK"
     gene            761..>1583
                     /locus_tag="PK717_08245"
     CDS             761..>1583
                     /locus_tag="PK717_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR47682.1"
                     /translation="MKIDKKTELNRDVYIAHAEIAGQEITVTTGKVAQQAGGSVMVQL
                     GGTVVLATAVVADKERPDIDFFPLVVDYFDKMYAAGKIPGGFFKRGGRPSDQEILRSR
                     LIDRPLRPLFPKHFRRDVQIVVSTLSSDGENIPDFLGIIGASCALTISDAPFEGPLAA
                     VRIGLIDGELVVNPTVTMLQKSTLDLVVAGTNDAIMMVEGGAIQVDEETVAKAMELAQ
                     ASFADIIALQMELQKEIGKPKIVVPPPEIDPEVLQLVHDELFSKGDEKLFNPDKAQ"
BASE COUNT          437 a          332 c          382 g          432 t
ORIGIN      
        1 ggaacaagtg gactcaaaaa gaggaggttt gccctgttgt aggcatcctc aagtttttca
       61 atgagttccc tgtctggtat gaatcttatc gtttctgcca taagcctgat ctgttccctg
      121 atggtcaatg ctctattgtt ttttgcaagc tcggtgatca ttttgcctgc ggcttcgtaa
      181 gcaaaggaaa cgtcagagat tgaaactcta atatcatgtc tgattagtat ttcatggatc
      241 ttttcaatcc tgtatttacc cctcttttcc tcaacatccg gcaagtcata aataagtgta
      301 ttccagaagt cgaagattac ggcttgcggt ttgtttttgg ttttaatcat aagttagaat
      361 tatagccgtt tgttttataa aggttcaaga ccagtccttg aatcggtaag ttttcgtgtt
      421 atattggcct aaccttggct aggatggacg tactccgcga cgacaccgtg gccacaggaa
      481 aggagaaaat attaatgtta gacaaagaca aaaagcggca gatcattgag aaatttgcca
      541 cccacgaagg tgatacaggt tcaccagaag tccaggttgc acttcttacc cagaggatca
      601 acgaactttc agctcacctg cttacccaca aacatgattt tacatcaagg agaggacttt
      661 tgaggatggt tggtgagaga cacagaatgc ttggttatct tcgttcaata gaagaaaaca
      721 ggtatctaaa gctcatcagt gccctcggac tgcgtaagta atgaaaatag acaaaaagac
      781 agagctaaat agagacgttt atattgccca tgctgaaata gcggggcagg aaataacagt
      841 caccaccggc aaagttgctc agcaggccgg tggttctgtt atggtgcaac tcggcggaac
      901 ggttgttctt gcaactgccg ttgtggctga taaagaaaga cctgacatag atttcttccc
      961 gctggttgtc gattatttcg acaaaatgta tgcggcagga aagatcccag gcgggttttt
     1021 caagaggggt ggaaggccgt ctgaccagga aatattgagg tcaagactta ttgatagacc
     1081 cctcaggccg ttgttcccaa agcatttccg cagagatgtc cagattgttg tttccacact
     1141 ttcgtcagat ggggagaaca tcccggattt tcttggaata attggtgctt cttgcgctct
     1201 caccatttct gatgccccgt ttgaagggcc actcgcagca gtaaggattg ggcttatcga
     1261 cggagagctt gttgtaaatc caaccgtcac tatgctccag aaatcaacgc ttgatctggt
     1321 ggttgctgga acaaacgatg caataatgat ggttgagggt ggcgcaatcc aggtggacga
     1381 agagacagtc gcaaaagcca tggagcttgc acaggcctcc tttgccgaca ttattgccct
     1441 gcaaatggag cttcaaaaag aaattgggaa gccgaagatt gttgttcctc cgccagagat
     1501 cgatccagaa gttttacaac tcgtccatga tgagcttttc agcaaaggtg atgaaaaact
     1561 tttcaatccg gacaaggcac agc
//
LOCUS       DAOSRI010000346         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_73323_length_1580_cov_4.390164, whole genome shotgun sequence.
ACCESSION   DAOSRI010000346 DAOSRI010000000
VERSION     DAOSRI010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73323_length_1580_cov_4.390164"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1580
                     /locus_tag="PK717_08250"
     CDS             <1..>1580
                     /locus_tag="PK717_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR47683.1"
                     /translation="LLDGEKFETKVTKGPSAGCNATFDVAQQKSPQSGNNAKFIIFWI
                     DKENRRPKKFEEMLKVHEAFDEISSFAFMVVTGDNDNVFFANNQARELFGLTKNPSDE
                     LFVSNYCVLPPQLKDALKDELKNTKDIASGQIEYKTKLGKIRHFRVVSKALELFGKPS
                     KILVLHDVTKLSQMERRLARYENLEKLIETLGSEISGKDSLDKYLEVTLELICKHFGL
                     ASGVIYFEELGDFVQKKIYGEKIGPMSEIHKFAIRKVFESGKPQVVGSAFATNDDVYY
                     LLPIFGSDGLTGVFSFRSQTLDENIINQFDTIMPVVASGIELCRMKTRESQYIEELER
                     LSSFRSDILESVNHEMKTPLTSILGYAELILSGTIKGSEQTINAANSIYAAGKQLETL
                     IGELSKLSYNTIVVPEMAVQEVNTREVLKTASNLMLPIFEAAVLSFRANIPEDLPWVK
                     ADPTKLKEILLNMLSNAAKYTESGGEITLSASVIGGNFIRMEVKDTGIGISSEKKRFI
                     FKKYYRQANEKTRKGLGL"
BASE COUNT          521 a          286 c          351 g          422 t
ORIGIN      
        1 tgcttttgga tggagaaaag tttgaaacaa aagtaacaaa gggtccctct gcaggttgca
       61 atgcaacctt tgatgtggct cagcaaaaat ccccccagag tggcaataat gcaaaattca
      121 taattttctg gattgacaag gaaaacaggc gcccaaaaaa atttgaagag atgttgaaag
      181 tccatgaagc atttgatgaa atatcatcct ttgccttcat ggtggttact ggtgataatg
      241 ataatgtgtt ttttgctaat aaccaggcaa gagagctttt tgggcttaca aaaaatcctt
      301 ctgatgaatt atttgtgtca aactactgcg tccttcctcc acaactaaaa gatgccctta
      361 aggatgaact taaaaacacg aaagatattg cctcgggcca gattgagtac aaaacaaaac
      421 tgggcaaaat aagacatttc agggtggttt ctaaagcatt ggaattattt ggaaaaccat
      481 caaaaatact tgttcttcat gatgtaacaa aattaagcca gatggaacga aggcttgcca
      541 gatatgaaaa cctggaaaag cttatcgaaa cattgggatc tgagatctct ggcaaagata
      601 gtctggacaa atatcttgag gttacccttg agttgatttg caagcatttt ggtttggcat
      661 caggagtgat ctattttgaa gaacttgggg acttcgtcca gaaaaagata tatggggaaa
      721 aaatcgggcc aatgtctgag atccacaaat ttgcgattag aaaggttttt gaaagtggga
      781 agccgcaagt agtggggtcc gcttttgcaa caaatgatga tgtttactac ttattgccga
      841 tctttggttc agatggctta acaggcgtat tttctttcag gtcccagacg ctcgatgaaa
      901 atattatcaa tcaatttgac acaataatgc ccgtggtggc ttcaggcatc gagctttgca
      961 ggatgaaaac aagagagtcc caatacatag aagagctcga aaggctgagt tctttcaggt
     1021 cggatatcct tgagtctgtc aatcacgaga tgaaaacacc tctgacctcg attttgggat
     1081 atgcagagtt aatactttct gggacaataa agggcagtga acagacgata aatgctgcga
     1141 actcgattta tgctgcagga aagcaattag aaacccttat aggggaactt tcaaagcttt
     1201 cctataatac aattgtggta ccagaaatgg ctgtgcagga agtcaataca agggaggtcc
     1261 ttaaaacagc ctcgaacctc atgctcccaa tatttgaagc agcagtgctg tctttcaggg
     1321 caaacattcc ggaagacctt ccatgggtca aagctgatcc cacaaaactt aaagagatcc
     1381 ttctgaatat gctttcgaat gctgcaaaat atacagaatc tggtggagaa ataacccttt
     1441 cagcatctgt tattggcgga aattttatca ggatggaagt aaaggacaca ggcatcggta
     1501 tttcaagcga gaaaaaacgt ttcatattca aaaaatatta caggcaggca aatgaaaaga
     1561 cacgtaaagg acttggcctt
//
LOCUS       DAOSRI010000347         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_73593_length_1576_cov_4.237344, whole genome shotgun sequence.
ACCESSION   DAOSRI010000347 DAOSRI010000000
VERSION     DAOSRI010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73593_length_1576_cov_4.237344"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK717_08255"
     CDS             <1..369
                     /locus_tag="PK717_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47684.1"
                     /translation="KEEKNNIPPNPLFEGRTDYDKTDNSDKGQQELQVLDEDHLAFDR
                     FWNLYDKKAGRVKCEKLFNNLSENDWNKIFETLPTYIQSTPDRQYRKHPETYLRNRAW
                     EDELYVKEQKEETLKVIPIL"
     gene            366..>1576
                     /locus_tag="PK717_08260"
     CDS             366..>1576
                     /locus_tag="PK717_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOR47685.1"
                     /translation="MNLEQNILGSIISGYEDLGDAANILTSAMFEKPAHQAIFSACLK
                     LYDQGKTIDLIAVNAALQGNDIYQKAGGAGYLAEVAQTIGGRLCEHALVLKERFVTRK
                     ALEMAKNINRAYTDQQPLDDILGLINNSNDEINGLLIDKNKSLPASDVMMQCMEQLEA
                     RTARAKNGDHLGIPTGLTKLDQLTNGWKGNQLIVLAARPGQGKTQLAIHFALTAAAHS
                     FSCIFFSLEMTSTALMDRALISMSKVSSEDYRAGKLTQAAWDKITQAGSHIQQMPITI
                     NDASLQTVRKIRTECLLLKRTQRLDMVIIDYLQLIEPEDRHQIREQQVAIMTRALKLL
                     AKELGVPVILLCQLNREAENNQGKRPSLANLRESGAIEQDADLVLLLLRPEAYGIKTI
                     DIGEQIGVSTAG"
BASE COUNT          514 a          346 c          362 g          354 t
ORIGIN      
        1 aaagaagaaa agaataatat accccctaac cccctttttg aagggaggac tgattatgat
       61 aaaaccgata attctgataa aggacaacag gagctccaag tactagatga agatcatttg
      121 gcttttgatc gtttctggaa tctctacgac aaaaaagccg gacgggtaaa atgcgaaaag
      181 ctcttcaata atttatctga gaacgactgg aacaaaatct ttgaaacctt accaacctat
      241 atccagagca caccagatag gcaataccgc aaacatcctg agacttactt gcgaaacaga
      301 gcatgggaag atgaactgta cgtaaaagaa caaaaagaag aaacgttaaa agtaattccc
      361 atactatgaa tttggaacag aatatattag gttcaataat atccggatac gaagacttgg
      421 gagatgctgc caatatactt acatcagcca tgtttgagaa accggcacac caggcaatct
      481 tctccgcgtg tttgaaatta tacgaccagg gaaaaacgat tgatctaatc gctgtaaatg
      541 cagcgttgca aggaaatgac atataccaga aagcaggggg agcaggatat cttgccgagg
      601 tggcccaaac cattggcggc cggttgtgcg agcatgcctt ggtgctcaaa gagcgtttcg
      661 taacacgcaa agctcttgag atggccaaaa acatcaatag agcctacacc gaccaacaac
      721 cactggatga tatcctggga ctgataaata acagcaacga tgagatcaat ggccttctta
      781 ttgataaaaa caaatcattg cctgcctccg atgttatgat gcaatgcatg gagcagctgg
      841 aagctcgcac tgcaagagct aaaaacggag accacctcgg catacctacc ggcttgacga
      901 agctggatca gttgacgaac ggatggaaag gaaatcaact catagtcctt gcagcgagac
      961 cgggacaggg taaaacacaa ttggctatcc acttcgcctt gacggcagca gcacacagtt
     1021 tttcctgtat ctttttctct ttagaaatga catctactgc tttgatggat cgtgccttga
     1081 tcagcatgtc aaaggtgtct tcagaagatt accgtgcggg aaaactgaca caggcggcct
     1141 gggataagat tacccaagcc ggatcacata ttcaacaaat gccgatcacc atcaatgatg
     1201 cgtctttgca gactgttcgc aaaatcagaa ccgagtgtct cctgcttaaa aggacgcagc
     1261 gtctggacat ggttattatt gattatttgc aattgataga gccggaagac agacatcaaa
     1321 tccgagaaca acaagttgcc ataatgacaa gggcattaaa actgcttgcc aaggagcttg
     1381 gagtcccggt catcctttta tgtcaattga accgggaggc agaaaacaac cagggaaaac
     1441 gtccttcttt ggctaacctg agagagtcag gagccataga gcaagatgcg gacctggtgc
     1501 tgctcttatt gcgccctgag gcgtacggca tcaagaccat tgatatcgga gaacaaatag
     1561 gagtctctac agcggg
//
LOCUS       DAOSRI010000348         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_73770_length_1574_cov_2.603028, whole genome shotgun sequence.
ACCESSION   DAOSRI010000348 DAOSRI010000000
VERSION     DAOSRI010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73770_length_1574_cov_2.603028"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PK717_08265"
     CDS             complement(<1..635)
                     /locus_tag="PK717_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR47686.1"
                     /translation="MLLGKGQIMVIDDSKPVLEVVTAMLEGAGYEVRQFLRARDALMD
                     LINGNPDLIICDILMPEMDGFEFRRALVEDQRTSHIPFLLLTGKDDLMLMIEGLSAGI
                     QDYIMKPIQPKEFLTHVGRIMDNIYEARFKLLDRVLIEGTLDNIEFEKIEKVLDAIKF
                     TGKLVLGSETREPLNIAYKRGRAIAAEGGPMAAKGHEAYKTAPTMSGEFEI"
     gene            complement(657..>1574)
                     /locus_tag="PK717_08270"
     CDS             complement(657..>1574)
                     /locus_tag="PK717_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR47687.1"
                     /translation="KNFQLPKLPLIVNPAALPIKVGTTGTILASTDETVKATLTPGKS
                     AIVKNIDDNEVLVYAFARGEELLTITSPSGETATCKLFCFADDGPESPSVYDFHAIPG
                     TKSARLVWKLPNVQRDPFKGLSVTLDGKKLTELGPDTASLQVTGLATGVTYKFEIVPQ
                     GGQKVSASCTTGPLDVKLELWIGKTDAYINGKKTKLAVPPQILSNSTYVPFRFLGEAF
                     GADVGWDNSKREASFWTPNAFLQMTVGSKTGYNFGAQVTINPPPQNVSGRVVIPLRAA
                     SELLGAKVAYEAKTKKITINYRKPWHLWW"
BASE COUNT          321 a          396 c          394 g          463 t
ORIGIN      
        1 attatctcga attcaccgga cattgtcgga gcggtcttgt atgcctcgtg cccttttgca
       61 gccatcggac caccctctgc agctatcgcc ctgccccttt tgtatgcaat gttaagtggc
      121 tcccttgttt ccgagccaag aaccagcttc ccggtgaatt ttattgcatc gagcactttt
      181 tctatttttt caaattcgat gttgtcgagc gttccctcaa tgaggaccct gtccagcagt
      241 ttgaacctgg cttcgtagat gttgtccatt atccttccaa catgggtcag gaactctttg
      301 ggctggatcg gtttcatgat gtagtcctgg attccagccg agagaccttc gatcatcagc
      361 ataaggtcgt ccttccctgt cagaagaaga aatgggatgt gcgaggtacg ctggtcttcg
      421 acgagtgccc tcctgaattc aaaaccgtcc atctctggca tgagtatgtc gcaaatgatg
      481 aggtcggggt tgccgttgat aaggtccatg agtgcgtccc gtgccctgag gaattgcctt
      541 acttcgtatc ctgcgccctc aagcatcgcc gtgacgactt caagcaccgg ctttgagtca
      601 tcgatgacca taatctggcc tttgcccagc agcatttttg cctccttatt gtgtgtttac
      661 caccagagat gccatggttt cctgtagttg attgtaatct tttttgtttt tgcttcgtag
      721 gccacttttg cacccaggag ctcggatgcc gctctgagtg ggataaccac tcttccggac
      781 acattttgtg gcggaggatt tatcgtcact tgcgcgccaa aattatagcc tgttttggaa
      841 cccacagtca tttgcaggaa ggcatttggt gtccagaatg aggcctccct ctttgagttg
      901 tcccagccaa catcggctcc aaatgcctct cccaggaagc ggaacggcac ataggtgctg
      961 tttgaaagga tttgtggcgg aacggccagt ttcgttttct ttccgtttat gtaggcatcg
     1021 gtttttccaa tccagagctc gagcttgaca tccagtgggc cagtcgtgca tgatgccgaa
     1081 accttctgcc ccccttgtgg gacaatctcg aatttgtatg ttacgccagt tgcaagacct
     1141 gttacctgca atgatgcggt atcggggcca agctcggtga gcttcttgcc atccagggtg
     1201 actgaaagcc ccttaaaggg atcacgttgg acatttggaa gtttccagac cagccttgct
     1261 gatttcgttc ctggtatcgc atggaaatca taaactgatg gagactctgg tccgtcatcg
     1321 gcaaaacaga acagtttgca ggtcgcagtt tcgcctgatg gcgaggtgat tgttagcaat
     1381 tcttcaccac gtgcaaatgc gtagaccaga acctcattgt catcaatatt tttgacgatt
     1441 gccgatttgc ctggtgtcag tgttgctttt acggtttcat cggtgctggc aaggattgtc
     1501 cctgttgtac caacctttat ggggagcgcc gcaggattga caatcagcgg cagttttggc
     1561 agctggaagt tctt
//
LOCUS       DAOSRI010000349         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_74502_length_1563_cov_2.936340, whole genome shotgun sequence.
ACCESSION   DAOSRI010000349 DAOSRI010000000
VERSION     DAOSRI010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74502_length_1563_cov_2.936340"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1563)
                     /locus_tag="PK717_08275"
     CDS             complement(<1..>1563)
                     /locus_tag="PK717_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOR47688.1"
                     /translation="GMTGVAAQMCGDRDVVASLGYQVKPDGTILQEETDENGKKIWKP
                     ISKLGARKAVLNDLSPAATFIAYNYNTPVDIKAFEQEAKRILAEVEDECGWMYETLHT
                     DGKTKGKINYTVWSDVFVCPECAEEVVFWDVAVDKESHSVKEEFICSHCSAKISKRNM
                     ERQWVTKYDIALQKTVKQARQIPVMIAYTLDSHKGRFEKKPDDKDLSLIEEIERKDIP
                     YWYPTHTIPEGEKTPETLRIGITHQHHLYQKRNLWIISCLFAKLKSKSLRLVFQSVVS
                     TLSSKLVRYNFGNRGNGILTGTIYVPSLNAETIILKVFAGKVRDFSKALKIREHSTIN
                     NGSCSNVPLSNSSVDYIFIDPPFGANLQYSELNLFWEGWLSVFTNNTEEAIESRAQKK
                     GSNDYRSLMAKCFNEAYRTLKPGRWMTVEFSNTKASVWNSIQTALTEAGFIVANVSAL
                     DKKQGSFNAVSNPTSVKQDLVISAYKPNGGFEERFKQEAQTEEGVWDFIRTHLKYLPV
                     TKKLGLDLVAIP"
BASE COUNT          417 a          355 c          294 g          497 t
ORIGIN      
        1 ccggaatggc aacaagatca agtcccagtt ttttagtcac cggaaggtat ttcaaatggg
       61 ttcgtataaa atcccagaca ccttcctcgg tctgagcttc ctgtttgaaa cgctcttcga
      121 agccgccgtt tggtttatag gcggaaataa cgaggtcttg ctttacagaa gttggattgc
      181 taacagcatt aaaactacct tgttttttgt ccaaggctga tacatttgcc acaataaacc
      241 ctgcctcagt aagagctgtc tgaatactgt tccacacaga ggcctttgta ttcgaaaact
      301 ctactgtcat ccagcgccct ggtttaagag tgcgatatgc ttcattgaag catttagcca
      361 tgaggctcct gtaatcattt gaaccttttt tctgtgctct actctcaata gcttcttccg
      421 tgttatttgt gaaaacactc agccagcctt cccagaatag atttaattca gaatactgga
      481 gatttgcacc aaatggtgga tcgataaaaa tgtaatctac agagctattt gatagaggta
      541 cattggagca acttccatta tttatggtag aatgttctct gatctttaat gccttggaaa
      601 aatccctgac tttacccgca aatactttga gaatgattgt ttcagcgttc aaagagggca
      661 catagatcgt accggtaagt attccgttac ctctgtttcc aaaattatat ctaaccaatt
      721 ttgatgacag agttgaaact acagactgaa aaacaagtcg cagactcttt gactttaatt
      781 ttgcaaataa acaagagatt atccacaagt tccttttttg gtaaagatgg tgttgatgag
      841 tgatgccgat tctcaaggtt tctggagttt tttcaccttc tggtattgtg tgagttgggt
      901 accagtaagg aatatcctta cgctctatct cctcaatcaa tgaaagatct ttatcgtcag
      961 gttttttttc gaaccgacct ttatgactat caagcgtata agctatcata actggaatct
     1021 gccgagcttg ctttaccgtt ttttgcaaag caatgtcgta ttttgtaacc cattgccttt
     1081 ccatgtttct tttggaaatt tttgcagagc aatgagagca aataaactcc tccttaactg
     1141 aatgtgattc cttgtcaacc gctacatccc agaacacaac ttcctctgca cactcaggac
     1201 aaacaaacac atcactccat acggtataat taatcttacc tttcgtcttt ccgtcagtat
     1261 ggagcgtttc atacatccag ccgcattcat cttccacttc ggcaagaatt cttttggctt
     1321 cctgttcaaa tgctttgata tctaccggcg tattatagtt gtaggcaata aatgtagcgg
     1381 caggagacaa gtcatttaat accgccttgc gagcaccgag tttcgagatt ggtttccaga
     1441 tctttttgcc attctcatca gtctcttcct gcaggattgt accgtctggt tttacctgat
     1501 aaccgagtga cgcaaccaca tcacggtcgc cgcacatctg cgcagccaca cctgtcatcc
     1561 cgg
//
LOCUS       DAOSRI010000350         1561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_74634_length_1561_cov_3.395086, whole genome shotgun sequence.
ACCESSION   DAOSRI010000350 DAOSRI010000000
VERSION     DAOSRI010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74634_length_1561_cov_3.395086"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1561
                     /locus_tag="PK717_08280"
     CDS             24..>1561
                     /locus_tag="PK717_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOR47689.1"
                     /translation="MRKISLILSLLVFLAPLSYPRFAQSAEQGGYLVIAAPGAEGVLQ
                     SWQKHRESQGYSVKIISSTDIIAGSSGIAPYFQLQNYVRDNQKKFNIKYLLLVGDTND
                     IPMPRLYAAKNVDNVVWGEPGPVYSDYFYSQPDTNWDRANDGRLGEPDDDQVQIAPKI
                     AVGRIPFSDQANIEKYLNRVKLYDSPSSSRKSVLQAAALYSFDNEDGDPTNKFTDGAD
                     MEEKIWSDILKPMGYIRKTLYECDGTKPGLPGDMCLNSTNLLNELTKNDYGMVNWLAH
                     GEANRIVRKYWLNDQNGNGHADSATEIRMPLLLDDGQLYNNIIKSRLIISTACSTADV
                     AGGASSLGSASLRSGAGAFVGATAINYFTPGWSKPSDGGNQTISYYITKNYAEGDAIG
                     FALAKSLESFAKEFRTVNGWQNKYLQNVYSFILLGDPGMKQEPVIPKQDIQMAFSPAS
                     VTVVSGQKAETKLVFGEVPAIYPLKLSYTTAYGITITFDPPDPLPNQDARVIVQVEKF
                     VVPQ"
BASE COUNT          440 a          378 c          373 g          370 t
ORIGIN      
        1 caacgtatgt aaaataggtg agaatgcgaa aaatctctct gatactctcg ctactggttt
       61 ttctggcgcc cctctcctac cctcgcttcg cacaatcggc ggaacagggg gggtatttgg
      121 tcattgcagc ccctggtgct gaaggcgtat tgcaatcgtg gcaaaaacat agggaatcac
      181 agggttattc agtaaaaatc atcagttcca cagacattat tgcggggagc agtggaattg
      241 caccttattt ccaactccag aattatgtca gggacaacca gaaaaaattc aatatcaagt
      301 accttttgct tgttggcgat acaaatgaca ttccaatgcc caggctctat gcggcgaaaa
      361 acgtggacaa tgttgtctgg ggggagcctg gcccggttta ttccgattat ttctattccc
      421 agccggacac caactgggac agggccaatg atggaagact tggggagccg gatgatgacc
      481 aggtccagat tgccccaaaa attgctgttg gaaggatacc cttttctgac caggcgaaca
      541 ttgagaagta tttgaacagg gtaaaactct atgactcgcc atcttcctca agaaaaagtg
      601 ttttgcaggc agcggcgctt tactcttttg acaatgagga tggtgaccca accaacaaat
      661 tcaccgatgg cgcggacatg gaagagaaga tctggagtga tatcctgaag ccgatggggt
      721 acatcagaaa gaccctctac gagtgtgacg gcacaaagcc tggtcttcct ggcgacatgt
      781 gccttaacag cacgaatttg ctgaatgaac tgacaaaaaa tgattatgga atggtcaact
      841 ggctggcaca cggcgaagca aacaggatcg tcaggaaata ttggctcaac gaccagaacg
      901 ggaatggcca cgcggatagt gccactgaaa tcagaatgcc actcctgctt gatgatggcc
      961 agctctacaa taatattatt aaatcaagac tcatcatctc cacggcttgc tcaacagctg
     1021 acgtcgcagg cggcgcctca tctttgggtt cggcttcctt gcgttccggc gctggtgcct
     1081 ttgttggcgc aacagcgata aattacttta cgccgggatg gagcaagccc tctgatggtg
     1141 gcaaccagac aatctcttac tacataacaa aaaattatgc cgaaggagac gccattggtt
     1201 ttgcgcttgc aaaaagcctt gaatcattcg caaaagagtt caggacagta aatggctggc
     1261 agaacaaata ccttcaaaat gtctattcat tcatcctgct tggcgaccca ggcatgaagc
     1321 aggaaccagt cattccaaaa caagatatcc agatggcttt ttctccagca tccgttacag
     1381 tagtctctgg ccagaaggca gaaacaaagc ttgtttttgg agaagtgccc gccatctacc
     1441 cgctcaaatt gtcatatacg actgcatatg gcatcaccat aactttcgac ccacctgacc
     1501 cgctcccaaa ccaggatgcc agggtcattg tgcaagtcga gaaatttgtt gtcccgcaaa
     1561 a
//
LOCUS       DAOSRI010000351         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_78042_length_1515_cov_3.761644, whole genome shotgun sequence.
ACCESSION   DAOSRI010000351 DAOSRI010000000
VERSION     DAOSRI010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78042_length_1515_cov_3.761644"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK717_08285"
     CDS             <1..654
                     /locus_tag="PK717_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47690.1"
                     /translation="KPEPVVSAAPKVEEAPPKVEYKPPQAPAATPTPTPVEPPVKKVE
                     PSPTPSGRPRLDFSNEDIDPFRPHGEFDDEVKKPPQHEEPPKTPSFAATQPPQQQPQN
                     IMDPPTTRIPVKPPAPSPSSPQATVVTGPSGKVAFLLDGIKKVAQSNVGEDIIPWLDG
                     QISRMQQIQPNLGKRDLLLLVDEIERYVRTVRQNPAKATKLASQLRHIIDSFAANLG"
     gene            complement(698..>1515)
                     /locus_tag="PK717_08290"
     CDS             complement(698..>1515)
                     /locus_tag="PK717_08290"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47691.1"
                     /translation="VPEALEYLAELYEEANRKNESQALVSSIQGRLRKKDFVALESEN
                     PGECRVLAVYAPQIVRSPDPAKPPERLFSRGSLGTLEVSYIPGGGGLLVTGNLGESFQ
                     NSIEVAYAFFKRHLESHGLVQNPDMDIHVDVPGWLPKYDGPSAGVALACSMISAFTGK
                     TIPKNVTMTGEISMHGKVMPVGGIKEKVEATYGKGIDKIFIPKENKWDYLDMLLKDTT
                     HEMESVEASQEEVGKNLPVVIAVDRIEQIVENLGIGIDESMIPKESTQEEPEK"
BASE COUNT          400 a          415 c          323 g          377 t
ORIGIN      
        1 aaaccagaac ctgttgtttc tgcagctcca aaggtggagg aagccccgcc aaaagtcgag
       61 tacaagccac cccaagctcc agcagcaact cccacaccta cccctgtgga accgccagta
      121 aaaaaagttg agccatcacc aacaccatca ggaaggccaa ggcttgattt ctccaatgag
      181 gatatagacc cattcaggcc acacggagaa ttcgacgacg aggtaaaaaa accaccccag
      241 cacgaagaac caccaaagac accgtctttt gctgccaccc agccgccaca gcaacagcca
      301 caaaatatca tggacccacc aaccacccgc attccagtca aaccaccagc cccgtcccca
      361 tcttcaccgc aggcaactgt tgtaacaggt ccttcaggca aagtggcttt tttgcttgac
      421 ggcataaaaa aggttgccca aagcaatgtt ggagaggaca tcattccatg gcttgatggc
      481 cagatatcca ggatgcagca gattcagcct aatcttggaa aaagagacct cctgttgctg
      541 gttgacgaga ttgaaagata tgtaaggact gtaaggcaga acccggcaaa ggcgacaaaa
      601 ctcgcctccc agctccgtca tattattgat agttttgcgg caaatcttgg gtagttccga
      661 aacaaacaaa aaggcccggc ctggaccggg ccttttttta tttctcaggt tcttcctgag
      721 tgctctcttt gggtatcatt gactcatcaa tgccaattcc aaggttctca actatttgct
      781 caatcctatc aacagcaatc actaccggga ggttcttgcc tacctcttcc tgagaggcct
      841 cgacagattc catttcatgg gttgtatcct tgagcaacat gtcaagataa tcccatttgt
      901 tttctttcgg aataaatatt ttgtcgatgc ctttcccata tgttgcctcg actttttcct
      961 ttattccgcc gactggcatc acttttccat gcattgatat ctcgccagtc atggtgacat
     1021 ttttgggtat cgttttgcct gtgaatgcgg agatcattga gcaggcaaga gcgactccag
     1081 ccgaagggcc atcatacttc gggagccagc caggaacatc aacgtgtatg tccatatcag
     1141 gattttgaac aagaccatgg ctctcaaggt gcctcttgaa gaaggcatag gccacttcaa
     1201 tgctgttttg gaaagattcg ccaaggttgc cggtcacaag taatccgcca ccacctggga
     1261 tgtaggagac ttcaagcgtt ccaagagacc ctcttgagaa aagcctctcc ggcggttttg
     1321 caggatcagg gcttctcact atctggggag catacactgc aagcaccctg cattctccag
     1381 ggttttcaga ttctagagcc acaaaatctt tttttctgag tctcccctgt attgacgaga
     1441 caagcgcctg cgactcgttc tttctgtttg cctcttcata aagttcagcc aaatattcca
     1501 gcgcttcagg tactt
//
LOCUS       DAOSRI010000352         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_78414_length_1510_cov_3.184192, whole genome shotgun sequence.
ACCESSION   DAOSRI010000352 DAOSRI010000000
VERSION     DAOSRI010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78414_length_1510_cov_3.184192"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="PK717_08295"
     CDS             complement(<1..1462)
                     /locus_tag="PK717_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOR47692.1"
                     /translation="MQILEISEVWGFVSYKVFNASTGTVYKLSADDICAEVPEENFNE
                     SYLRYVAMLSKIKNETSGGILSKLSSGIIPLPHQLHVLNRALSNNNVRYILADEVGLG
                     KTIEAGLIIKELKTRGLIKRILVVCPTGLVTQWSLEMQDKFNEKFHVILPEDYDTIRK
                     ITGNEDVYGQFDQVISPMDSIKPLERRVGWTEEKIAEYNEERIYSIINSGWDLVVIDE
                     AHRVAGSSGEVARYKLGNLLAAASPYLLLLTATPHNGKTEPFLRLIRLVDEKAFPNIN
                     AIVKEQVAPYVIRTEKREAIDNNGNKLFKNRTTKAIELHWDERHSMQRRLYEMVSDYV
                     SKNYNKAMRNRGKNMWFVFLLIMMQRLVTSSTSAVRESMERRVKVLEEQAFKYQSMSE
                     EEFAEMELEENMEDAIAAISLDIKEEIAQLNDIIAVAKQAEFQYLDVKVEPLLEIIDD
                     LFAEDRQRKVIIFTEFVATQSYLVKLLKDRGYSTSIL"
BASE COUNT          384 a          368 c          306 g          452 t
ORIGIN      
        1 tgagaatgga tgttgaatac cccctgtctt tcagcagctt tacaaggtag ctttgggtcg
       61 ccacaaattc cgtaaaaatg ataacctttc tttgccggtc ctcagcaaat aagtcatcga
      121 taatttcaag caacggctca actttgacat caagatattg gaactctgcc tgcttggcaa
      181 cggcaatgat atcgttaagc tgggcaattt cttctttgat gtccagagaa atggccgcaa
      241 tggcatcctc catgttttct tccaattcca tctctgcaaa ttcttcttcg gacatggatt
      301 gatacttgaa tgcttgctct tctaggactt taacacgccg ttccatactt tcccggacgg
      361 cgcttgtgct gctggtgacc agacgctgca tcataatgag caggaatacg aaccacatgt
      421 ttttgccgcg gttgcgcatt gccttgttat aatttttgga aacgtaatcc gacaccattt
      481 catacagcct gcgctgcatg gaatgccgtt cgtcccagtg caattcaata gccttggtag
      541 ttcgattttt aaaaagctta ttgccgttat tgtctatggc ttcacgcttt tcagtcctga
      601 taacataagg agctacctgc tctttaacaa tggcattgat attgggaaaa gccttttcat
      661 caaccaaacg tatgagccgc aaaaacggct ccgttttacc attatgaggt gtagctgtca
      721 aaaggagcag gtaagggctg gctgcagcca ggaggttgcc aagcttgtat ctggctactt
      781 cccctgaact cccggcaacc ctatgggcct cgtcaatgac gaccaaatcc cagccgctgt
      841 tgattattga gtaaatccgt tcctcgttat actctgcaat cttttcttct gtccagccaa
      901 cccgtctttc caagggcttg atagaatcca tgggtgagat aacctgatca aactgcccgt
      961 aaacgtcctc atttccagtt atcttacgaa tggtgtcata atcttccggc aggataacat
     1021 ggaacttctc gttaaacttg tcctgcattt ccagactcca ttgagttaca agaccggtag
     1081 ggcagacaac cagtatccgc ttgataagtc ccctggtctt taactcttta ataatgagtc
     1141 ctgcttcaat agtttttccg agacccactt catccgccaa tatgtatctc acattattgt
     1201 ttgataatgc ccggttaaga acatgaagct ggtgcggcaa aggaatgatg ccgcttgata
     1261 gttttgacag gatgcctccg gaggtttcgt tcttgatttt tgaaagcatg gcaacatacc
     1321 gaagatagct ctcattaaaa ttttcttcgg gtacttcggc gcagatgtca tcggcggata
     1381 acttataaac tgtcccggta gaagcattaa acaccttgta agacacaaag ccccatactt
     1441 cgctgatctc caatatctgc acacgctcat ttttagtttt atcaaaaacg taatctccta
     1501 tgatcattcg
//
LOCUS       DAOSRI010000353         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_78783_length_1505_cov_3.791724, whole genome shotgun sequence.
ACCESSION   DAOSRI010000353 DAOSRI010000000
VERSION     DAOSRI010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78783_length_1505_cov_3.791724"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PK717_08300"
     CDS             <1..815
                     /locus_tag="PK717_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47693.1"
                     /translation="AAFVVSIYFSLFLSEIIMALSFKNPPSLTKSILSFIYWPSVPNI
                     ITVALFLVFWMFIAAPALAGAYRFLIGTVDPKHIAQRTFINGIVFYTKRMLSYTSFLV
                     LLFSLFLGAMYGLQILMEAIFVPQIAINWIYLVMLILAYMVLTKFSYAPVLIISGEGI
                     FGALKRSWVATSRRFWAIFGIRLLILFISIFPVLIVYFTNFTVTPSMSLWPYFFLMSL
                     GFEEFIMIRGLQEWEEERNLPIISPQFFDRDLRYLEVYIKQHDYDYETKDME"
     gene            complement(799..>1505)
                     /locus_tag="PK717_08305"
     CDS             complement(799..>1505)
                     /locus_tag="PK717_08305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR47694.1"
                     /translation="MKSKPSLVVLHGLGASSEWMGMYKKSFSDNYNVIIPDMPYSPDS
                     GPHCGRSITGYVDYVYELLKDTIRVEGSILIGESLGGAVAMMLCQKISFKKLVLIAPS
                     LGIEKGFISTYLRMLLVPVDILAEPVAEKIAKSDPELGVLSKKIMVSTPKYKMLNPLV
                     AMKRFSAFDLGSDCLPELLVIGGKYDKVVPEDKLRLFASKHGANLYINNESGHHVAVN
                     RWPDVEARIKEFIPYL"
BASE COUNT          411 a          351 c          285 g          458 t
ORIGIN      
        1 gagctgcatt cgtagtctca atctatttct cgctcttcct gtctgaaata ataatggccc
       61 tctctttcaa aaaccctcca agccttacca aaagcatcct ttcttttatc tactggccga
      121 gtgtaccaaa catcattaca gtagcgctgt ttctggtctt ctggatgttc atagcggcac
      181 cagctcttgc tggcgcatac aggtttctca taggaacggt cgaccccaag cacattgcac
      241 agagaacatt catcaatgga atagtattct atacaaaaag gatgctttct tacacaagtt
      301 ttcttgtcct tcttttctca cttttccttg gcgcaatgta cggattgcaa atccttatgg
      361 aagcgatctt tgttccacaa attgccatca attggatata cctcgtcatg ctgatactgg
      421 catacatggt tttgaccaaa ttttcatacg ctccggtgtt gatcatctcg ggagaaggca
      481 ttttcggggc attgaagcgc tcatgggtgg caacatccag aaggttctgg gcaatctttg
      541 gaataaggct ccttatactg ttcatttcta ttttcccagt gctgattgtc tattttacaa
      601 actttaccgt gaccccttcc atgagcctct ggccatactt tttcctgatg tctcttggat
      661 ttgaggaatt cataatgata agaggactcc aggaatggga agaagagcgc aatctgccaa
      721 tcatttcacc acaattcttt gacagggacc tcaggtatct tgaagtgtac atcaagcaac
      781 atgactacga ttacgaaact aaagatatgg aataaattct tttatcctag cctcgacatc
      841 aggccacctg ttcacagcaa catggtggcc tgattcatta tttatgtaca aattggcgcc
      901 atgtttgctt gcaaacaacc tgagtttatc ttctgggaca accttgtcgt actttcctcc
      961 gataacaagt aattctggga gacagtctga tcccaaatca aaagcgctaa atcttttcat
     1021 ggcaacaagc ggatttaaca ttttgtattt gggtgttgaa accatgatct tcttggaaag
     1081 tacccccaac tccggatcag atttggcaat cttttctgct acaggctctg caagaatgtc
     1141 aacgggaaca agcaacatcc tcaaatatgt tgatataaaa cccttttcaa ttccaaggct
     1201 tggtgcaatg agaaccagtt ttttaaagga tattttttga caaagcatca tcgcaacagc
     1261 accaccgaga gattctccaa ttaggataga cccttctact ctgatagtgt cttttaataa
     1321 ttcgtacaca tagtccacat aacctgtaat actcctgccg caatgcgggc cggaatcagg
     1381 cgaatagggc atatctggga taattacgtt gtagttatcg gaaaaggatt ttttatacat
     1441 ccccatccac tcggagctcg ctccaagccc atgcaacaca actaaagatg gttttgattt
     1501 catgt
//
LOCUS       DAOSRI010000354         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Caldisericia bacterium isolate AMR_MDS_2271
            NODE_78929_length_1503_cov_4.790746, whole genome shotgun sequence.
ACCESSION   DAOSRI010000354 DAOSRI010000000
VERSION     DAOSRI010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864338
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caldisericia bacterium (wastewater metagenome)
  ORGANISM  Caldisericia bacterium
            Bacteria; Caldisericota/Cryosericota group; Caldisericota;
            Caldisericia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:32:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,663
            CDSs (total)                      :: 1,620
            Genes (coding)                    :: 1,608
            CDSs (with protein)               :: 1,608
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Caldisericia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78929_length_1503_cov_4.790746"
                     /isolate="AMR_MDS_2271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2758719"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK717_08310"
     CDS             <1..284
                     /locus_tag="PK717_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR47695.1"
                     /translation="SGYIDEQEKSIYLVASKKNRSSDGLKIKIVGVDEIDHKCYCAAD
                     NNGEVFLLHPPSFIKCKDIKLFSCYWIEGSFDKERHEYFATDMVLEPCE"
     gene            424..1431
                     /gene="gap"
                     /locus_tag="PK717_08315"
     CDS             424..1431
                     /gene="gap"
                     /locus_tag="PK717_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOR47696.1"
                     /translation="MARIAINGFGRIGRQVYKAISEYFPQHDIVAVNDITDPETLVHL
                     LKYDSNYGVYGHEITLEKDTFTCCGKRTRCLKVLSPLELPWNEMKVDIVVESTGKFAN
                     GKDAKVHLDRGARKVMVSAPMKDPDYMILRGVNCDYYDPKIHHVVSNASCTTNSLAPV
                     AKVLHENFGILNGFMTTVHAYTNDQKILDAPHKDLRRARNAATNIIPTTTGAAEAVGK
                     VIPELKGRMTGISLRVPTSVVSITDFVCTLEKQCNVEQINEKLEEASKGKLKGILGYS
                     REPLVSSDYKGSTYSGIVDALSTMVVGNNMAKVLIWYDNEWGYSVRSAEVVDILATRG
                     V"
BASE COUNT          452 a          307 c          356 g          388 t
ORIGIN      
        1 ttagcggata tattgatgag caagaaaaaa gtatctattt ggtagcatcg aagaaaaata
       61 gatcatcaga tggcctaaag ataaaaattg ttggtgttga tgaaattgac cataaatgtt
      121 attgtgctgc tgacaataat ggagaagtgt ttcttctcca tcctccatct tttataaagt
      181 gcaaagatat taagttattt tcctgctatt ggattgaagg ttcatttgat aaggaaagac
      241 acgagtattt tgctacagat atggtcctcg agccttgcga atagaaatac tcttacctta
      301 taaaaaatta ttaccagaaa aatggttaat tgcgaaattt ttgaattcgt gtggatttgc
      361 ctagaatttt gcgactgttg aaaaatattc tttggcgcgc ataatcggtt ctggaggtga
      421 aagatggcta gaattgctat caacggattc ggaaggattg gaaggcaagt ctacaaggcc
      481 atttccgaat atttcccaca gcacgacata gttgcggtca atgacataac agaccctgaa
      541 acgctcgtcc atcttctcaa gtatgacagt aactatggcg tttacggtca tgagatcact
      601 cttgaaaaag acacctttac atgttgcggc aagcggacca ggtgcctgaa agttctttcc
      661 ccgcttgagc tcccctggaa tgagatgaag gtggacatcg ttgtggagag caccggcaag
      721 tttgcaaacg gaaaggacgc gaaagtccat cttgacagag gcgcaagaaa agtcatggtc
      781 tctgccccga tgaaagaccc ggactacatg atcctccgcg gtgtgaactg cgactactac
      841 gacccaaaaa ttcaccatgt tgtttcaaat gcttcctgca caacaaacag cctggcgcct
      901 gttgccaaag ttcttcacga gaactttggc atcctgaatg gttttatgac gacagtccac
      961 gcctacacaa atgaccagaa gattcttgat gctccccaca aggacttgag aagggccagg
     1021 aatgccgcta caaatataat tccaacaaca accggcgctg ccgaggctgt tggaaaagta
     1081 atccctgaac tgaaaggcag gatgactggt atctccctca gggtgccgac gtctgttgtt
     1141 tcaataactg attttgtatg cactctggaa aaacagtgca atgtggagca gataaacgaa
     1201 aaacttgaag aagccagcaa aggaaaactg aaagggatcc ttggatactc cagagagcct
     1261 ctcgtttcca gtgattacaa gggttcgacc tattccggaa ttgtcgatgc actctcaacg
     1321 atggttgttg gcaacaacat ggccaaggtg cttatatggt acgacaatga atggggctat
     1381 tcagtcagat ctgcagaagt cgtcgacata ctggcaacaa gaggtgtgtg agtgaagaag
     1441 cgcactgttg aaggcgttga tgttttcaac aaaagggtgc tgctcagggc cgacttcaat
     1501 tcg
//
